LOCUS       DATIBB010000001         6104 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_526328, whole genome shotgun sequence.
ACCESSION   DATIBB010000001 DATIBB010000000
VERSION     DATIBB010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 6104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6104
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_526328"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..313
                     /locus_tag="VLC50_00005"
     CDS             <1..313
                     /locus_tag="VLC50_00005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF25877.1"
                     /translation="GSWKHAAALLDWGFAHAAQVSPVGQLVEPARPTPAASPSTRSPA
                     PPAANAAAAERSTVTEAPPTALVVLGALVVLTTGLLFRVRQVARRRRERARWRPPRPT
                     R"
     gene            complement(225..1208)
                     /locus_tag="VLC50_00010"
     CDS             complement(225..1208)
                     /locus_tag="VLC50_00010"
                     /inference="COORDINATES: protein motif:HMM:NF015587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HSF25878.1"
                     /translation="MSRIGSLLSGARSGLADLQARRPAIRHLVAAIHHYGAIHGNQHA
                     AAISYFAFLSFFPLVALGFAVLGFLGRSRPDLVAEMLDTVRNYLPGLVGDGPGQINLS
                     SIASSAAGASLIGFVGLFYAGLGWVDALRTGLRDVWDTVTPRRNLVVRKLIDVAVLVV
                     LGLSLLASVAISSVATSATRLVLDWLDLADGFVATGSVRVLAAVAALISDGVVLFVLF
                     RRLPGRPVGWRTALRGSLLGVVLLETLKLVGTLLLGRVTTNPVYGTFAVVVGLMIWLS
                     LVSRAVLLSAAWAVTGPGGPADVSESAAADASGPARGDGGPPGGPETAGPS"
     gene            complement(1233..1757)
                     /locus_tag="VLC50_00015"
     CDS             complement(1233..1757)
                     /locus_tag="VLC50_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020554602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HSF25879.1"
                     /translation="MLERTIGVAVAIPEPFGPELQGWREAFGDPLARAIPPHVTLLPP
                     TVVADADLAAIEAHLCEVAGRSTAFPMHLRGTGTFRPVSPVVFVALVEGIAGCERLES
                     AIRSGPLQRELSFYYHPHVTVAHQLADDALDEAFDKLAGYDARFVVDGFSLFEHGADE
                     IWRPRLRFRFGGPR"
     gene            complement(1822..2880)
                     /gene="trpS"
                     /locus_tag="VLC50_00020"
     CDS             complement(1822..2880)
                     /gene="trpS"
                     /locus_tag="VLC50_00020"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015796516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HSF25880.1"
                     /translation="MCPASAPAGPLASSSAPAQPRVLSGIQPTADSFHFGNYLGAIRQ
                     WVSMQDDHDAFYCVVDLHAITVEHDPAQLRARTLRSAAQLLAAGVDPDRCTLFVQSHV
                     PEHAELAWVLGCLTGFGEASRMTQFKDKSAKATSGGGPTVGLFTYPILQAADILLYQA
                     DRVPVGEDQRQHLELTRDLAQRFNTRFGRTFQVPEPFIVKATAKITDLQSPSAKMSKS
                     ASSTAGIIDLLDEPAVSAKKIRSAVTDTEREIRFDPEAKPGVSNLLTIYSALSGREIS
                     ALERDYAGKGYGDLKKDLAELVVDFVTPFRARTMALLEDREALDAVLARGAERARSVA
                     SETLADVYDKVGFVPAKH"
     gene            complement(2915..4231)
                     /locus_tag="VLC50_00025"
     CDS             complement(2915..4231)
                     /locus_tag="VLC50_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012950390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HSF25881.1"
                     /translation="MSDTLASIGLVLVFILIGGFFAAAEIALVSLREGQVRTLGDRGR
                     RGAKVAALHEDPNRFLAAVQVGVTLAGFLSAAFGAATLADDLAPVLRGWGLSDGAATA
                     IALVSITLVISYFSLVLGELAPKRLALQRAEGIALAVAPLLDRMARLSRPVIWLLSVS
                     TDLVVRLLGGNPQAAREVISEEELRDMVAGHESLTREERRLIDEVFDAGERQLHEVMV
                     PRTEVDFLDASLPVYKAAKQVGGSQRSRYPVVRGSHDEVVGFVHVRDLFVPEMAGRGV
                     RLEDIVRDVMMLPGTKKVLPALQEMRREGHHLAIVLDEYGGTDGIVTLEDLVEELVGD
                     IRDEYDVERDDARRLLGGGMDVDGLLNLEDFADRTGLALPEGPYETVAGFVMTTLGQL
                     PEVGDTVDAIGHRFTVTELDGRRVARLRVTTAPGATPGQPHDRAAG"
     gene            4391..5659
                     /locus_tag="VLC50_00030"
     CDS             4391..5659
                     /locus_tag="VLC50_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014981318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF25882.1"
                     /translation="MPAQLVVMGSGETAPTMVKVHREVFSAVPAGRAVALDSPYGFQV
                     NADDLSVRTARYFHDSVGHDIEVIRWRREARPPAAEEAALASVRTAAWVFAGPGSPTY
                     ALSAWHDTPMARTLADVPGRGGTLLLASAAAVATGAYAVPVYEVYKCGADPVWHEGLD
                     VLGAVTGVHAAVIPHWDNHEGGTYDTRFCYLGEGRLQALEDMLPAETVVLGVDEHTAL
                     LVDLESRTARVLGNRTVTVRRGQAQRVLAAGAVVPMEALGDPGERHRSRPSAAADEPS
                     ASVAEVSAPSLRGAADEAEAAFEAALAGRDVDRCVHLALELEQTIVDWSADTNESDDT
                     DHARRVLRTMLTRLGELARVGARDPREVVGPYVELALDLRERARTGHDWAASDAVRDR
                     LALLGIEVRDTPDGVEWVLAEQPAEPAQTS"
     gene            complement(5647..>6104)
                     /locus_tag="VLC50_00035"
     CDS             complement(5647..>6104)
                     /locus_tag="VLC50_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018560810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HSF25883.1"
                     /translation="PTGEADTARQAEKERFLDGLLDHLVSLRHSAREVLVAGDWNIAH
                     READLKNWKANQARAGFLPSERAWLDRLYQEAGYVDVVRTLEPEGPGPYTWWSYRGRA
                     FDTDAGWRIDLQVATPGLAARAVKAEVDRAPTYADRWSDHAPVVVTYDV"
BASE COUNT          874 a         2188 c         2249 g          793 t
ORIGIN      
        1 ggggtcgtgg aagcacgccg cggcgctgct cgactggggc ttcgcccatg ccgcccaggt
       61 gtccccggtc ggtcagctcg tcgagccggc ccgtcccacg cctgcggcga gtcccagcac
      121 taggtcgccc gctcctcccg ccgcaaatgc ggcggccgcc gagcgctcaa ccgtcaccga
      181 ggcgcccccc accgcgctcg tcgtgctggg tgccctcgtc gtcctcacga cgggcctgct
      241 gtttcgggtc cgccaggtgg cccgccgtcg ccgcgagcgg gcccgctggc gtccgccgcg
      301 gccgactcgc tgacgtctgc ggggccaccc ggaccggtca ccgcccaggc ggcgctgagc
      361 agcacagcac ggctgaccag gctgagccag atcatcagcc cgaccacgac cgcgaaggtg
      421 ccatagaccg ggttcgtggt cacgcggccg agcaggagcg tgccgacgag cttgagggtc
      481 tcgagcagca ccacgcccag cagggagccg cgcagcgccg tgcgccaccc gacgggccgg
      541 cccggcaacc ggcggaagag cacgaacagc acgaccccgt cgctgatgag ggccgccacc
      601 gcggcgagca cccgcacaga gccggtggcg acgaagccgt cggccaggtc cagccagtcc
      661 aggaccagcc tggttgccga ggtggccacg ctcgagatgg ccacggatgc caacaggctc
      721 aggcccagaa ccaccagcac ggccacgtcg atgagcttgc ggaccaccag gttgcgtcgt
      781 ggcgtgaccg tgtcccacac gtcacgcagg cccgtgcgca gcgcgtcgac ccagcccagc
      841 ccggcataga acaggccgac gaacccgatc agcgaggctc cggcggccga gctggcgatc
      901 gaggacaggt tgatctggcc cgggccgtcg ccgaccaggc cgggcagata gttgcgcacc
      961 gtgtcgagca tctcggcgac caggtcgggg cgtgagcgac ccaggaagcc cagcacggcg
     1021 aagcccagag ccaccaaggg gaagaaggac aggaacgcga agtaggagat cgccgccgcg
     1081 tgctggttgc cgtggatcgc gccgtagtgg tggatcgccg cgacgaggtg gcggatcgct
     1141 ggccggcgcg cctgcaggtc cgccaacccg gaccgggcgc cggagagcag cgagccgatg
     1201 cggctcaccg cgacctggtc accaaggccc gctcagcgcg ggccaccgaa ccggaaccgc
     1261 aggcgcggac gccagatctc gtcggcgcca tgctcgaaga gcgagaaccc gtcgaccacg
     1321 aagcgggcgt cgtagccggc cagcttgtcg aacgcctcgt cgagcgcgtc gtcggccaac
     1381 tggtgggcga ccgtcacgtg ggggtggtag tagaacgaca gctcgcgctg cagcggcccc
     1441 gaccggatcg ccgactccag ccgctcgcac ccggcgatgc cctccaccag cgcgacgaac
     1501 acgaccgggg agaccggccg gaaggtgccg gtgccgcgca ggtgcatggg gaacgcggtg
     1561 ctgcgcccgg ccacctcgca caggtgcgcc tcgatcgcgg cgaggtcggc gtcggcgacc
     1621 acggtcggcg gcaacagcgt cacgtgcggg gggatcgccc gggcgagcgg gtcgccgaag
     1681 gcttctcgcc acccctgcag ctccgggccg aacggctcgg gaatggcgac ggcgacgccg
     1741 atggtgcgct ccagcacggc gggcgggcct cccctcaggt ggcggccggc ccgagcgtcg
     1801 cgggccgggg tcgggacggt ctcagtgctt ggccggcacg aagccgacct tgtcgtagac
     1861 gtcggccagc gtctcggagg ccacgctgcg cgctcgctca gcgcctcgag ccaggaccgc
     1921 gtcgagggcc tcacgatcct cgagcagggc catggtccgg gcccggaagg gggtgacgaa
     1981 gtcgacgacc agttcggcca ggtccttctt gaggtcgccg tagcccttgc cggcgtagtc
     2041 gcgctcgagc gcgctgatct ctcgtccgga cagcgcggag tagatcgtca gcaggttgga
     2101 gacgcccggc ttggcctcgg ggtcgaagcg gatctcacgc tcggtgtcgg tgaccgcgct
     2161 gcggatcttc ttcgccgaga cggcgggctc gtcgagcagg tcgatgatgc cggcggtcga
     2221 ggacgcggac ttgctcatct tggccgacgg cgactgcagg tcggtgatct tggcggtcgc
     2281 cttgacgatg aacggctcgg gcacctggaa cgtgcggccg aaccgggtgt taaaccgctg
     2341 ggccaggtcg cgggtcagct cgaggtgctg gcgctggtcc tcgcctacgg ggacgcggtc
     2401 ggcctggtag agcaggatgt cggcggcctg caggatcggg taggtgaaca agccgaccgt
     2461 cggcccgccg cccgaggtgg ccttggcgct cttgtccttg aactgcgtca tccggctggc
     2521 ctcgccaaag ccggtgaggc agcccaggac ccaggccagc tcggcgtgct cgggcacgtg
     2581 gctctgcacg aacagcgtgc accggtcggg gtcgacgccg gcagcgagca gctgggccgc
     2641 cgagcgcagg gtgcgcgcgc gcagctgggc cgggtcgtgc tcgacggtga tcgcgtgcag
     2701 gtcgacgacg cagtagaacg cgtcatggtc gtcctgcatg ctcacccact gccggatcgc
     2761 cccgaggtag ttgccgaagt ggaacgagtc ggcggtcggc tggatccccg acagcacccg
     2821 cggctgggcg ggcgcactcg acgacgcgag tggtcctgcc ggagcggatg cggggcacat
     2881 gcgaggcatt gtgccagtca ccggtcgggc cgggtcagcc tgccgcccgg tcgtgcggct
     2941 ggccgggggt cgcgcccggc gccgtggtga ctcgcagccg tgccacccgg cgtccgtcga
     3001 gctcggtgac cgtgaaccgg tggccgatgg cgtcgacggt gtcgccgacc tcgggcagct
     3061 ggcccagagt cgtcatgacg aagccggcca ccgtctcgta agggccctcg ggcagcgcaa
     3121 gcccggtgcg gtcggcgaag tcctcgaggt tgagcagccc gtcgacgtcc atcccacccc
     3181 cgagcagccg acgggcgtcg tcgcgctcga cgtcgtactc gtcgcggatg tcaccgacca
     3241 gctcttcgac gaggtcctcg agggtgacga tgccgtcagt tccgccgtac tcgtcgagga
     3301 cgatcgcgag gtgatggccc tcccggcgca tctcctgcaa ggccggcagc accttcttgg
     3361 tgccgggcag catcatcacg tcacggacga tgtcttccag tctgacgccg cgcccggcca
     3421 tctccgggac gaacaggtcg cgcacgtgca cgaacccgac cacctcgtcg tgcgagccgc
     3481 gcaccaccgg gtagcgcgag cgctggctgc cgcccacctg cttggccgcc ttgtagaccg
     3541 gcagcgacgc gtcgaggaag tcgacctcgg tccgcggcac catcacctcg tgcagctggc
     3601 gctcaccggc gtcgaagacc tcgtcgatga gccggcgctc ctcccgggtc agcgactcgt
     3661 gcccggcgac catgtcgcgc agctcctctt cggagatgac ctcccgggcc gcctgcgggt
     3721 tgcccccgag caggcgtacg acgagatcgg tcgacaccga cagcagccag atcaccgggc
     3781 gggacagccg ggccatccgg tcgagaagcg gagccaccgc aagcgcgatg ccctcggccc
     3841 gctgcagggc cagccgcttg ggcgccagct caccgagcac gagcgagaag taggagatca
     3901 ccagcgtgat cgacaccagc gcgatcgcgg tggccgcccc gtcagacagg ccccacccac
     3961 gcagcaccgg cgccaggtcg tcggccaacg tcgcggcgcc gaaggcggcc gacaggaagc
     4021 cggccagcgt cacgccgacc tgcacggcgg cgaggaaccg gttggggtcc tcgtgcaggg
     4081 cggcgacctt ggcgccccgc cggccgcggt cgcccagcgt gcgcacctga ccctcgcgca
     4141 gcgagaccaa ggcgatctcg gcggcggcga agaagccccc gatgaggatg aagaccagga
     4201 cgaggccgat gctggccagc gtgtcgctca cggcgtgccg cccgccctac gccatcggcg
     4261 gcacgcccga ctactcgggt catccatgcc ggccagcata cggacggttc gcgcatgatc
     4321 gctgcgggct cagggtcggg gcgccggcga ggtgcttgcg ctcctgggct tgatcgggga
     4381 ggtttccggg gtgcccgctc agctcgtcgt catggggtcc ggcgagaccg cccccaccat
     4441 ggtcaaggtc caccgcgagg tgttctccgc cgtgcccgcg gggcgggcgg tcgccctcga
     4501 ctcgccctac ggcttccagg tcaacgccga cgacctgtcg gtgcgcaccg cccgctactt
     4561 ccacgacagc gtcgggcacg acatcgaggt aatccgctgg cgtcgcgagg cacggccgcc
     4621 ggccgccgag gaggccgccc tggcgagcgt gcggacggcg gcctgggtgt tcgccggccc
     4681 gggcagcccg acctatgcgc tgtcggcctg gcacgacacg ccaatggccc gcaccctggc
     4741 cgacgtaccc ggtcgtggcg gcacgctcct gctggccagc gccgccgcgg tggccaccgg
     4801 ggcctacgcc gtgccggtct acgaggtcta caagtgcggt gccgacccgg tctggcacga
     4861 ggggctcgac gtgctcggcg cggtcaccgg cgtgcacgcc gcggtgatcc cgcactggga
     4921 caaccacgag ggcggcacct atgacacccg cttctgctac ctcggcgagg gtcggctgca
     4981 ggcgcttgag gacatgctgc cggccgagac cgtggtgctg ggcgtcgacg agcacaccgc
     5041 gctgctggtc gacctggaga gccgtacggc gcgggtgctg ggcaaccgca ccgtgacggt
     5101 gcgccggggc caggcgcagc gggtgctggc cgccggagcc gtcgtaccga tggaggccct
     5161 tggggacccg ggtgagcgcc accggtcacg cccgtcggcc gcggccgacg agccgagcgc
     5221 ctccgtggcg gaggtctccg cgccgagcct gcgcggcgcc gccgacgagg cggaggcggc
     5281 gtttgaggcg gccctggccg gccgcgacgt cgaccggtgc gtgcacctgg cgctcgagct
     5341 tgagcagacc atcgtcgact ggtcggccga caccaacgag tccgacgaca ccgaccacgc
     5401 ccgccgggtg ctgcgcacga tgctcacccg gctcggcgag ctcgcgcggg tgggcgcccg
     5461 cgacccgcgc gaggtggtcg gcccctacgt cgagctggcc ctcgacctgc gcgagcgggc
     5521 ccgcaccggc cacgactggg cggcctccga cgcggtgcgc gaccggttgg cgctgctggg
     5581 catcgaggtg cgcgacaccc ccgacggggt ggagtgggtg ctggcagagc agccggccga
     5641 gccggctcag acgtcgtagg tgaccacgac cggggcgtgg tcgctccacc ggtcggcgta
     5701 ggtcggggcc cggtccacct cggccttgac cgcacgcgcc gccagccccg gggtggccac
     5761 ctgcaggtcg atgcgccagc cggcgtcggt gtcgaaggcc cggccgcggt aggaccacca
     5821 ggtgtaggga ccggggccct ccggctcaag cgtgcggacc acgtcgacgt agccggcctc
     5881 ctggtagagc cggtcgagcc aggcgcgttc ggacggcagg aacccggccc gggcctggtt
     5941 ggccttccag ttcttgaggt cggcctcgcg gtgggcgatg ttccagtcgc cggccaccag
     6001 cacctcgcgc gcgctgtggc gcaggctcac caggtggtcg agcagcccgt cgaggaagcg
     6061 ttccttctcg gcctggcggg ccgtgtcggc ctcgcccgtc ggga
//
LOCUS       DATIBB010000002         3046 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279 k141_280,
            whole genome shotgun sequence.
ACCESSION   DATIBB010000002 DATIBB010000000
VERSION     DATIBB010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3046
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_280"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            250..492
                     /locus_tag="VLC50_00040"
     CDS             250..492
                     /locus_tag="VLC50_00040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF25884.1"
                     /translation="MTEQLLTRLPASRWMWVIGAALVPWLNLALVWAWDTDEWRTDGV
                     PIGEVLNRAAVSLAVLLSIAHGGRRRCAARRRDRSR"
     gene            518..1084
                     /locus_tag="VLC50_00045"
     CDS             518..1084
                     /locus_tag="VLC50_00045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF25885.1"
                     /translation="MTWLVVGIPLSTAVWVYAALQSGLDRLGRRHLTLQPYRGDRSLG
                     LRPVGSLAFTGFWMLFGVIGPLLLTGFTDLPGMIVGGAVLVAGVALFFVSLRRLNRRM
                     VAVKQQELDRARALYAEAYERLRDDASLQVLGQQASLLSAAEALEKRAEQIQEWPFDE
                     AILARVVTIASSVAAVIIARLILDPVGL"
     gene            complement(1088..2083)
                     /gene="rlmB"
                     /locus_tag="VLC50_00050"
     CDS             complement(1088..2083)
                     /gene="rlmB"
                     /locus_tag="VLC50_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012782991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HSF25886.1"
                     /translation="MAGNSARRGAKRTPGSKKGAVVGSGGQRAKALRGKGPTPPAEAR
                     TGHPKARRAAASARRDAPATRRTPPNGHASARSRAAKGSAEMVAGRNAVVEALRAGVP
                     ATTLYLAARVDVDDRVREALKAASDRGLAMLETTRVDLDRLTGGALHQGIALQVPAYD
                     YAHPADLLTAAAEAGEVPLVVALDGVTDPRNLGAVVRSTAAFGGHGVLVPERRAAGMT
                     ASAWKTSAGAAARIPVARATNMTRALDELKKAGAFVVGLDADGDVALDELELAADPLV
                     LVVGSEGKGLSRLVREACDLVVRIPMSAATESLNAGVAAGVALYEVARARRRATR"
     gene            complement(2083..>3046)
                     /locus_tag="VLC50_00055"
     CDS             complement(2083..>3046)
                     /locus_tag="VLC50_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011757447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DALR domain-containing protein"
                     /protein_id="HSF25887.1"
                     /translation="APYGELSRQRLDDMEPAADADPRGKRDPRDFALWKGHKPTEPDS
                     ASWPTPWGRGRPGWHLECSAMAARYLGPAFDIHGGGLDLRFPHHENELAQSRAAGQPF
                     ASYWMHNAWVTTAGEKMSKSLGNSLLVDEVVRRVRPIELRYYLVATHYRSTIEYSEGA
                     LAEHGETFRRIEGFLERAQERTGPVAPGVVCAEFEAAMDDDLAVPAALAALQGVVREG
                     NALLAAGDLTGLRGAVASVRAMLGVLGIDPYDETWAARAGSGDARLHEVTDRLVHALL
                     EARAEARARRDFAAADAVRDQLKAAGVAVEDTPAGPRWSIAEED"
BASE COUNT          374 a         1136 c         1100 g          436 t
ORIGIN      
        1 gtcctcgagg cccgccatag ctcccggtca atccccacat gctgatgtgc catgctgaca
       61 tgccagtcaa acatgcgtca tcgtaggggc gcgtccgcac cgggtcaaga gtcagctgct
      121 caggccggcg ccacacagcg cgactggtgg gatcctgacc cgatcggagg aggtacgccg
      181 gcgttcgcac tgtgaggcgg tcgacggatg ggtcgcgaac cagcatccag cctttcttcg
      241 gcctcgccgt tgaccgagca gctgctgact cggctgcccg cgtcgcgctg gatgtgggtc
      301 atcggcgcgg cgttggtgcc ctggctgaat ctggccctgg tctgggcgtg ggacaccgat
      361 gagtggcgaa ccgacggcgt acccatcggc gaagtgctca accgggcggc ggtcagcctc
      421 gccgtcctgc tctcgattgc tcacggcggt cgtcgccgct gtgctgcccg ccgacgagat
      481 cgcagccggt gaggccggcg cggcgaccat ccaggcggtg acgtggctcg tcgtagggat
      541 cccgctcagc accgccgtct gggtctacgc cgccctgcag tcaggcctgg accgactggg
      601 acgcaggcat ctcaccttgc agccctaccg cggcgaccgc agcctcgggc tacgaccggt
      661 cgggagcctg gcgttcactg gcttctggat gctcttcgga gtcattggcc cgctgctgct
      721 cactggattc actgacctcc cgggaatgat cgtcgggggt gcggtgctgg tcgccggggt
      781 cgccctgttc ttcgtgtcgc tccgacgact caaccgtcga atggtggcgg tcaagcagca
      841 agagctcgac cgggcccgag ccctctacgc cgaggcctac gaacggctcc gggatgacgc
      901 ctcgctgcag gtgctggggc agcaggccag cctgttgagc gcggcagagg cgttggagaa
      961 gcgggccgaa cagatccagg agtggccctt cgatgaggcc atccttgccc gggtggtgac
     1021 catcgcctcc agcgtcgcgg cggtgatcat cgctcggctg atcctcgacc cggtcggcct
     1081 gtaggggcta gcgggtggcc cgccggcggg cgcgggcgac ctcatagagc gctaccccgg
     1141 cggcgacgcc ggcgttgagc gactcggtgg ccgcggacat cgggatgcgt acgacgaggt
     1201 cgcaggcttc gcgcacgagc cgggacaggc ccttgccctc ggagccgacg accagcacca
     1261 gcgggtcggc ggccagctcg agctcgtcga gcgcgacgtc gccgtcggcg tccaggccca
     1321 ccacgaacgc cccggccttc ttcagctcgt ccagcgcgcg cgtcatgttg gtcgcccggg
     1381 ccaccgggat ccgggccgcc gcaccggcgg aggtcttcca cgccgaggcg gtcatcccgg
     1441 ccgcccgacg ctcgggcacc agcacgccgt gcccgccgaa cgcggccgtc gagcgcacga
     1501 ccgcgcccag gttgcgcggg tcggtgaccc cgtcgagcgc gacgaccagc ggcacctcac
     1561 cggcctcggc ggcggcggtc agcaggtcgg cggggtgcgc gtagtcgtag gccggcacct
     1621 gcagcgcgat cccctggtgc agggcgccgc cggtgagccg gtcgaggtcg acccgggtgg
     1681 tctcgagcat cgccagcccg cggtcgctgg ccgccttgag cgcctcgcgc acccgatcgt
     1741 cgacgtcgac gcgtgcggcc aggtagagcg tcgtggccgg cacaccggcc cgcagcgcct
     1801 caacgaccgc gttgcgcccg gccaccatct cggccgagcc cttcgccgcc cgcgaccgcg
     1861 ccgacgcgtg gccgttgggc ggcgtacggc gcgtcgcagg cgcgtcacgc cgggcactcg
     1921 ccgcggcccg gcgcgccttc gggtgcccgg tgcgtgcctc cgccggtggg gtcggcccct
     1981 tgccgcgcag ggccttggcg cgctgcccgc cgctgccgac caccgcaccc ttcttgctgc
     2041 ccggggtgcg cttggcgccc cggcgggcgc tgttgccagc catcagtcct cctcggcgat
     2101 cgaccagcgc gggccggccg gggtgtcctc gaccgcgacg cccgcggcct tgagctggtc
     2161 gcgcaccgcg tcggcggccg cgaagtcgcg ccgggcccgg gcctcggccc gggcctcgag
     2221 cagggcgtgc accagccggt cggtgacctc gtgcagccgc gcatcgcctg agccggcgcg
     2281 cgcggcccac gtctcgtcat acgggtcgat cccgagcacc ccgagcatcg cccgcaccga
     2341 ggcgaccgcc ccgcgcaggc cggtcaggtc gccggcggcc agcagcgcgt tgccctcgcg
     2401 gaccaccccc tgcagcgcgg ccagcgcggc cggcacggcg aggtcgtcgt ccatcgccgc
     2461 ctcgaactcg gcgcacacca cgcccggcgc gaccggcccg gtgcgctcct gggctcgctc
     2521 gagaaagccc tcgatccgcc ggaacgtctc gccgtgctcg gccagcgcgc cctcggagta
     2581 ctcgatggtg ctgcggtagt gggtggccac caggtagtag cgcagctcga tcgggcggac
     2641 ccggcgcacc acctcgtcga cgagcagcga gttgcccagc gacttgctca tcttctcgcc
     2701 ggcggtggtg acccaggcgt tgtgcatcca gtaggacgcg aacggttgcc cggccgcgcg
     2761 cgactgggcc agctcgttct cgtggtgcgg gaagcgcagg tccagcccgc caccgtggat
     2821 gtcgaaggcc ggcccgagat agcgcgcggc catcgccgag cactccaggt gccagccggg
     2881 ccggccccgg ccccacggcg tcggccacga cgcggagtcg ggctcggtcg gcttgtggcc
     2941 cttccacagc gcgaagtcgc gcgggtcacg cttgccccgc gggtcggcgt cggcggccgg
     3001 ctccatgtcg tcgaggcgct ggcgcgagag ctcgccgtac ggcgcc
//
LOCUS       DATIBB010000003         3423 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_1842549, whole genome shotgun sequence.
ACCESSION   DATIBB010000003 DATIBB010000000
VERSION     DATIBB010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3423)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3423)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3423
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1842549"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..527
                     /locus_tag="VLC50_00060"
     CDS             <1..527
                     /locus_tag="VLC50_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020550888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HSF25888.1"
                     /translation="ITAGVASIKGTYEGEVALADQQPPSTFVLRASGAGAPGTVQADV
                     TVTLAPDGSGGAGTLLHYDADAVVGGMVGGVGQRMLTGVAKKTAGEFFKAVDDVLTGA
                     APAVAPAAAEPAVVGAPAAVGEAGPKVFAAPAKPAGALAGGAPWWQLLAAFGFGAFWG
                     LAGVVIGSRIARRR"
     gene            647..2740
                     /locus_tag="VLC50_00065"
     CDS             647..2740
                     /locus_tag="VLC50_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013416651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HSF25889.1"
                     /translation="MARRIRIGIDTGGTFTDVVAVDEATGEMVTTKTPSTPADPAEGF
                     MTGVHKVLAQLGVGGEAVSAVSHGTTVATNKLLEHKVENLGFITTEGYEHLLEIARQS
                     VPDGYGNSYFWVKPDRIVPADRVRTVGGRLAVDGSEIRPFDEASAVAAARFFREHGIS
                     TIGVCFLHAYANPAHERAMLAVLEREHPEATVSISSQVLREYREYERAITTLVDAAVK
                     PNIRRYVANITARLDAFTTGEDATAHRVPFYVMKSNGGVLSAEEVVHQPITTVLSGPA
                     AGALGAAVVAERAGFSSVVTLDGGGTSTDVTVVVDGAPALTTEGRIGSYPSKIPMIDV
                     VTVGAGGGSIAWVSPGGALKVGPQSAGADPGPLCYRKGGTEPTITDAHVLLGRIPPHL
                     LGGEIPLDVDAAREGVAKLAGGLGLGLEQCAVGILEISAWNQANALRQITVKRGLDVR
                     DFTMVTFGGSGSLLACRLADILGLRSVLVPLNPGNTSAYGLLTVDVRNDYVRTAVARH
                     RGLDLAAMGQVLDGLAAEADAALEREGFDPATRQFPRTADLRYFGQAFEVRVDVPDGP
                     LTQELVDAVAQAFHAEHRKLYGYDFADDERQQVEWVNLRVTGVGPIRKPELREIAAGT
                     GAEAARTGTRQVYFDDWVATGIYDRARLGAGDVVAGPAVLEEFSSTIPVHPGFTATVD
                     RFGNVLMTRTTEVSQ"
     gene            2737..>3423
                     /locus_tag="VLC50_00070"
     CDS             2737..>3423
                     /locus_tag="VLC50_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020550903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HSF25890.1"
                     /translation="MTSTRPAPYRLTHGVDVDPVLVEIVEGYLASVEMEVETAIGRTS
                     RSPMIRDAHDYRAGIHDRHLRKLTGRSYSALVHPVARDYPLETMRPGDVFFHNDVYLS
                     EGGIGHLPDLCVTAPVFVTDPATGDDVVVAFVQAFGHHDDIGGAVPGSMPSHATSVFE
                     EGLMVPPIRLWDAGVPNEAALKIMTRNSRMPESLAADLDAECAACLMGARRLGELFDR
                     YGRDAVEECFD"
BASE COUNT          478 a         1232 c         1240 g          473 t
ORIGIN      
        1 cgatcaccgc aggggtcgcc tccatcaagg gcacctacga gggcgaggtg gcgctggccg
       61 atcagcagcc accgagcacg ttcgtgctgc gggcctctgg cgccggagcg ccgggcacgg
      121 tgcaggcaga cgtcacggtg accctggctc cggacggctc cggtggcgcg ggcacgctgc
      181 tgcactacga cgccgacgcg gtcgtcggcg ggatggtcgg cggggtcggc cagcggatgc
      241 tcaccggggt ggccaagaag acggccggag agttcttcaa ggcggtcgac gacgtgctga
      301 ccggggccgc cccggcggtg gcaccggcgg ccgccgagcc ggccgtggtc ggcgcgccgg
      361 ccgccgtggg ggaggccgga ccgaaggtgt tcgccgcgcc ggccaagccg gctggtgcgc
      421 tcgccggcgg cgcgccgtgg tggcagctgc tcgcggcgtt cggcttcggg gcgttctggg
      481 ggctcgccgg ggtggtcatc ggctcgcgga tcgctcgccg ccgctgaggc tgcccgccga
      541 gcgggtgggt gttgccaaca accgcccgat ccttggggtg aagttgccga ggtcgagccg
      601 aagtctcgcc gattcaatgg cgacagactt cgagggaggg ccgcccatgg cacgtcggat
      661 ccggatcggc atcgacaccg gcgggacgtt cactgacgtc gtcgccgtcg acgaggcgac
      721 cggcgagatg gtcacgacca agacgccatc gacgccggcc gaccccgccg aggggttcat
      781 gacgggcgtg cacaaggtgc tggcccagct cggcgtcggc ggggaggccg tcagcgcggt
      841 cagccacggc accacggtgg ccaccaacaa gctgctcgag cacaaggtcg agaacctcgg
      901 cttcatcacc acggagggct acgagcacct gctcgagatc gcccgccagt cggtgcccga
      961 cggctatggc aacagctact tctgggtcaa gcccgaccgg atcgtgccgg ccgaccgggt
     1021 gcgcacggtg ggcggacgcc tggccgtcga cggcagcgag atccgcccgt tcgacgaggc
     1081 cagcgccgtg gccgcggccc gcttcttccg cgagcacggc atctcgacca tcggggtgtg
     1141 cttcctgcac gcctacgcca acccggcgca cgaacgggcc atgctcgcgg tgctcgagcg
     1201 tgagcacccc gaggccacgg tgtcgatctc ctcgcaggtg ctgcgcgagt atcgcgagta
     1261 cgaacgggcc atcacgacgc tcgtcgacgc cgcggtcaag cccaacatcc gccgctacgt
     1321 cgccaacatc accgcacgac tggacgcgtt cacgaccggc gaggacgcga ccgcacaccg
     1381 ggtgccgttc tacgtcatga agtccaacgg cggcgtgctc tcggccgagg aggtcgtcca
     1441 ccagccgatc acgacggtgc tctccggccc cgcggccggt gccctggggg cggcggtcgt
     1501 ggccgagcgg gccggcttct ccagcgtcgt caccctcgac ggcggcggca cctcgaccga
     1561 cgtcacggtg gtcgtcgacg gcgcgccggc gctgaccacc gaggggcgga tcggctccta
     1621 cccgagcaag atcccgatga tcgacgtggt cactgtcggc gcgggcggcg ggtcgatcgc
     1681 ctgggtcagc cccgggggcg cgctcaaggt gggtccgcag tcggccggcg ccgatcctgg
     1741 cccgctgtgc taccgcaagg gcggcaccga gccgaccatc accgatgcgc acgtgctgct
     1801 cggccggatc ccgccgcacc tgctgggcgg agagatcccg ctcgacgtcg acgcggctcg
     1861 cgagggcgtc gccaagctgg ccggtgggct cggcctgggt cttgagcagt gcgcggtggg
     1921 catcctcgag atctcggcgt ggaaccaggc caacgcgctg cgccagatca ccgtcaagcg
     1981 gggcctggac gtgcgcgact tcacgatggt caccttcggg ggatcggggt cgctgctggc
     2041 ctgccggctg gccgacatcc tcggtctgcg cagcgtgctc gtgccgctca acccgggcaa
     2101 cacctcggcc tacggattgc tcaccgtgga cgtgcgcaac gactacgtgc gcacggcggt
     2161 ggcccgccat cgcggcctcg acctggccgc gatgggtcag gtgctcgacg ggctggccgc
     2221 cgaggccgac gccgccctgg agcgggaggg gttcgacccg gcgacccgcc agttcccgcg
     2281 caccgccgac ctgcgctact tcgggcaggc cttcgaggtc cgggtcgacg tgcccgacgg
     2341 cccgctgacc caggagctcg tcgatgcggt cgcgcaggcg ttccacgccg agcaccgcaa
     2401 gctctacggc tacgacttcg ccgacgacga gcgtcagcag gtcgagtggg tcaacctgcg
     2461 cgtcaccgga gtgggcccca tccgcaagcc cgagctgcgc gagatcgccg ctggcaccgg
     2521 cgccgaggcg gcccgcaccg gcacccgcca ggtctacttc gacgactggg tggcgaccgg
     2581 catctacgac cgggcccggc tgggcgccgg cgacgtcgtg gccggcccgg cggtgctcga
     2641 ggagttcagc tcgacgatcc cggtgcaccc gggcttcacg gccaccgtcg accggttcgg
     2701 caacgtgttg atgacccgca cgacggaggt ctcccagtga ccagcacccg ccccgcgccg
     2761 taccgactca ctcacggggt cgacgtcgac ccggtgctgg tcgagatcgt cgagggctac
     2821 ctcgcctcgg tcgagatgga ggtcgagacc gcgatcgggc gcaccagccg ctcaccgatg
     2881 atccgcgacg cccacgacta ccgggccggc atccacgacc ggcacctgcg caagctcacc
     2941 ggccggtcct actccgcgct ggtgcacccg gtcgcgcgtg actacccgct ggagaccatg
     3001 cgtcctggcg acgtgttctt ccacaacgac gtctacctgt ccgagggcgg catcggccac
     3061 ctgcccgacc tgtgcgtcac ggcgccggtg ttcgtcaccg acccggccac cggcgacgac
     3121 gtcgtggtcg cgttcgtgca ggccttcggc caccacgacg acatcggcgg cgccgtgccc
     3181 gggtcgatgc cctcgcacgc caccagcgtg ttcgaggagg gcctgatggt gccgccgatc
     3241 cggctgtggg acgccggggt gcccaacgag gccgcgctga agatcatgac ccgcaactcg
     3301 cggatgcccg agtcgctggc cgccgacctc gacgccgagt gcgccgcgtg cctcatgggt
     3361 gcgcgccggc tgggggagct gttcgaccgg tacgggcgcg acgctgtcga ggagtgcttc
     3421 gac
//
LOCUS       DATIBB010000004         3075 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279 k141_1102,
            whole genome shotgun sequence.
ACCESSION   DATIBB010000004 DATIBB010000000
VERSION     DATIBB010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3075
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1102"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..853)
                     /locus_tag="VLC50_00075"
     CDS             complement(<1..853)
                     /locus_tag="VLC50_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011292980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HSF25891.1"
                     /translation="MSTVLVANRGEIAVRVVRACRDAKLGSVAVYAEADRDALHVRLA
                     DQAFSLDGSTPAESYLSIDKLIDVARRSGADAVHPGYGFLAENAAFAQAVLDAGLVWI
                     GPPPAAIDALGDKVKARHIAEKVGAPLVPGTSDPVSGADEVVAFARDHGLPVAIKAAF
                     GGGGRGLKVARTLEEIPELFESAVREAVSAFGRGECFVERYLDHPRHVETQCLADTHG
                     NVVVVSTRDCSLQRRHQKLVEEAPAPFLSDEQMATLYAASKAILREAGYVGAGTCEFL
                     VGLDGTIS"
     gene            complement(912..1364)
                     /locus_tag="VLC50_00080"
     CDS             complement(912..1364)
                     /locus_tag="VLC50_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007927374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HSF25892.1"
                     /translation="MSAQHDGHRPAGADRSEATWSIAEIAEDFGVTLRTIRFYEDRGL
                     ITPERRGTARVFHPRDRVRLELVLRGKRLGFSLDEIARIVDMYDAEPGEVGQLAYLLE
                     QIGVRRAELQQRRRDIEASLAELDDVERRAQADLRRLRRAAKGATAPA"
     gene            1472..2638
                     /locus_tag="VLC50_00085"
     CDS             1472..2638
                     /locus_tag="VLC50_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012919075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HSF25893.1"
                     /translation="MSFDLSEDHESFRKVVRDFAEAEIAPHAAQWDREHHFPLDVVAA
                     MGTLGLFGLTAPEEYGGGGGDFTSLCVAIEEIGRVDQSMGITLEAAVGLGINPILTFG
                     SDEQKQRWLPDLVTGRALAGFGLTEPGAGSDAGSTRTKAVLDGDEWVIDGAKQFITNS
                     GTEITSCVTVTARTGPDVDGRAEVSAFIVPTGTPGFTVEPAYDKLGWHASDTHGLTFE
                     GARVPASALLGERGKGFGQFLATLDDGRIAIAALALGCAQACLEMSVRYAGERASFGV
                     PIGAKQGLAFQISDIAVMVETSRLLTYKAAWLKDSGAPRARLKQAAAIAKLHATESAV
                     TATRIATQVFGGYGFMEEYPVARFYRDAKVLEIGEGTSEIQRMLIARGLGLPVK"
     gene            2678..>3075
                     /locus_tag="VLC50_00090"
     CDS             2678..>3075
                     /locus_tag="VLC50_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020578661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HSF25894.1"
                     /translation="MAGDPDRLAVVAAAREQTLAPSPAGAAKLARQRKLYVRERIDLL
                     FDAGTFVEDGQLANALAAGLPADGVVTGRGLVEGRPAIVVANDPTVKAGSWGARTVEK
                     IIRLTELALRDELPVFWLVDSAGARITDQV"
BASE COUNT          425 a         1079 c         1130 g          441 t
ORIGIN      
        1 acgagatggt gccgtcgagg ccgacgagga actcgcaggt gccggcgccg acgtagccgg
       61 cctcgcgcag gatcgccttg ctggcggcgt agagcgtggc catctgctcg tcggacagga
      121 acggtgccgg ggcttcctcg acgagcttct ggtggcgtcg ctgcagcgag cagtcgcggg
      181 tggagaccac cacgacgttg ccgtgggtgt cggccaggca ctgggtctcg acgtggcgcg
      241 ggtggtcgag gtagcgctcg acgaagcact cgccacggcc gaacgccgag acggcttcgc
      301 gcaccgccga ctcgaacagc tcggggatct cctcgagcgt gcgggccacc ttgagcccgc
      361 gcccgccgcc gccgaaggcg gccttgatgg cgaccggcag cccgtggtcg cgggcgaacg
      421 cgaccacctc gtcggcgccg ctgaccgggt cggaggtgcc gggcaccagc ggggcaccga
      481 ccttctcggc gatgtggcgg gccttgacct tgtcgccgag cgcgtcgatc gccgccggcg
      541 ggggaccgat ccagaccagc ccggcgtcga gcaccgcctg ggcgaacgcg gcgttctccg
      601 cgaggaaccc gtagccgggg tgcaccgcat cggcgccgga gcgacgggcc acgtcgatca
      661 gcttgtcgat cgacaggtag gactcggccg gggtggagcc gtcgagcgag aaggcctggt
      721 cggccagccg cacgtgcagc gcgtctcggt cggcctcggc gtagaccgcc acgctgccaa
      781 gcttggcgtc ccggcacgcg cgcacgacgc gcaccgcgat ctcgccacgg ttggcgacca
      841 gcaccgtgct gatcgggcgg gtggcaacgc tgggctcgct cacgctctcc tcctcgtagt
      901 cgtcgcgcag tctaggccgg tgctgtggcg cccttggcgg cccggcgcag gcggcgcagg
      961 tcggcctggg cgcgccgctc gacgtcgtcg agctcggcca ggctggcctc gatgtcacga
     1021 cggcgctgct gcagctcggc gcgccgtacg ccgatctgct cgagcaggta ggcgagctga
     1081 cccacctcgc ccggctctgc gtcgtacatg tcgacgatcc gggcgatctc gtcgagcgag
     1141 aaccccagcc gcttgccacg cagcaccagc tcgaggcgga cccggtcgcg ggggtggaag
     1201 acccgggcgg tgccccggcg ctccggcgtg atcaggccgc ggtcctcgta gaagcggatc
     1261 gtgcgcagcg tgacgccgaa gtcctcggcg atctcggcga tcgaccaggt cgcctcgctt
     1321 cggtcggcac ctgcgggccg gtgcccgtcg tgctgtgcgc tcatcgggtc cccttgcggt
     1381 acgacggcga ctcggcttac gttgacgtaa gcgtaaaccg cgtcgtacga ctcagccaac
     1441 cccgccggcc gcacccgaaa ggccaccggt catgagcttt gacctgtccg aggaccacga
     1501 gtccttccgc aaggtcgtgc gcgactttgc cgaggcagag atcgccccgc acgccgcaca
     1561 gtgggaccgc gagcaccact tcccgctcga cgtcgtcgcc gcgatgggca cgctcgggct
     1621 gttcggcctg accgctccgg aggagtacgg cggtggcggc ggcgacttca ccagcctgtg
     1681 cgtggccatc gaggagatcg gtcgggtcga ccagtcgatg ggcatcacgc tcgaggccgc
     1741 tgtcgggttg ggcatcaacc cgatcctgac ctttggctcc gacgagcaga agcagcgctg
     1801 gctgcccgac ctggtcaccg gccgggcgtt ggccgggttc gggctgaccg agccgggcgc
     1861 cggctcggac gccgggtcga cgcggaccaa ggcggtcctc gacggcgacg agtgggtcat
     1921 cgacggggcc aagcagttca tcaccaactc gggcacagag atcacctcgt gcgtcacggt
     1981 caccgcgcgc accggtcccg acgtcgacgg gcgggccgag gtctcggcgt tcatcgtgcc
     2041 gaccggcacg cctgggttca ccgtcgagcc ggcctacgac aagctcggct ggcacgcgtc
     2101 tgacacccac gggctgacgt tcgagggcgc ccgggtgccc gcctcggcgc tgctggggga
     2161 gcggggcaag gggttcggcc agttcctcgc caccctcgac gacggccgca tcgcgatcgc
     2221 cgccctcgcc ctcggctgcg cgcaggcctg cctggagatg tcggtgcgct acgccggcga
     2281 gcgggcctcc ttcggcgtac cgatcggagc caagcagggg ctggcgttcc agatcagcga
     2341 catcgcggtg atggtggaga cgtcgcggct gctcacctac aaggccgcct ggctcaagga
     2401 ctccggcgcg ccccgggcgc ggctcaagca ggccgcggcg atcgccaagc tgcacgccac
     2461 cgagtccgcg gtcaccgcca cccggatcgc cacccaggtc ttcggcggct acgggttcat
     2521 ggaggaatat ccggtggcgc gcttctatcg cgacgccaag gtgctcgaga tcggtgaggg
     2581 cacgagcgag atccaacgga tgctcatcgc ccggggcctc gggctgccgg tcaagtaggg
     2641 tccggctgtg tctcagggcg agccgcagga gagcaccgtg gctggcgatc ccgaccgcct
     2701 cgccgtcgtt gccgcggcgc gtgagcagac ccttgccccg tcgcccgccg gcgccgccaa
     2761 gctggcccgg cagcgcaagc tctatgtccg cgagcgcatc gacctgctct tcgacgcggg
     2821 caccttcgtc gaggacggcc agctggccaa cgcgctggcc gccgggctgc cggccgacgg
     2881 ggtggtgacc gggcgggggc tggtcgaggg ccgaccggcg atcgtggtgg ccaacgaccc
     2941 cacggtgaag gccggttcgt ggggggcgcg cacggtcgag aagatcatcc ggctgaccga
     3001 gctggcgctt cgcgacgagc tgccggtgtt ctggctggtg gactcggccg gggccaggat
     3061 caccgaccag gtgca
//
LOCUS       DATIBB010000005         5306 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_1843323, whole genome shotgun sequence.
ACCESSION   DATIBB010000005 DATIBB010000000
VERSION     DATIBB010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 5306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5306)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5306
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1843323"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..155)
                     /locus_tag="VLC50_00095"
     CDS             complement(<1..155)
                     /locus_tag="VLC50_00095"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HSF25895.1"
                     /translation="MPTDEASELIGLTRELARDVLTPVAAVHEAESRFPRELVRTLGE
                     AGLLGLP"
     gene            complement(175..1284)
                     /locus_tag="VLC50_00100"
     CDS             complement(175..1284)
                     /locus_tag="VLC50_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020573859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HSF25896.1"
                     /translation="MPPTAISPDRIDRARTACTQAGVDALLVTPGPDLRWLTGYDAIP
                     LERLTCLVVPAQGDAFLVVPRLEVSAALASPVRGLDLEVVAWDETDDPVAVLTHRLGA
                     VATVALSDQMWAEKVLRFREALPQAQQVLGSVVLRDLRMRKAPEEVEALRAVGAAIDR
                     VHARIGEWLRAGRTEREVGRDIAAAILAEGHDTVDFVIVGSGPNGASPHHEQSDRVIE
                     TGDPVVIDIGGSMGGYCSDSTRTYAVGAPPAEFVDYYAVLQAAQAAACEHVRPGVSAE
                     SVDAAARTVINDAGWGKEFFHRTGHGIGVETHEEPYIVAGNALPLEPGMAFSVEPGIY
                     LEGRHGARIEDIVVVTEDGIERLNRSTRDLVVLEG"
     gene            1388..2284
                     /locus_tag="VLC50_00105"
     CDS             1388..2284
                     /locus_tag="VLC50_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019986123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease"
                     /protein_id="HSF25897.1"
                     /translation="MLLDTASLYFRAFFGVPDSLRAPDGTPVNAVRGLLDFIARLVTD
                     RAPTRLVACWDDDWRPVFRVAAIPSYKAHRVADEAANAEDVPDLLSPQVPIIVDVLAA
                     LGIARVGAPGHEADDVIGTLATQATCPVDVVTGDRDLFQLVDDAGEVRVLYTARGVGK
                     HDVVDEAEVRRRYGVPARAYADFATLRGDPSDGLPGVAGVGEKTAASLLTTYGDLPGV
                     LAALDDPKSGLSPTLRRRLDAARDYLAVAPAVVRVVTDLDLPAVDDRLPRAPADLEGL
                     AELATRWNLGGSVQRVVAALAA"
     gene            complement(2289..5024)
                     /locus_tag="VLC50_00110"
     CDS             complement(2289..5024)
                     /locus_tag="VLC50_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003959843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HSF25898.1"
                     /translation="MSSPAERFAAARRRRTEAASALGRFRALYDFPLDGFQVSACEAL
                     EAGQGVLVAAPTGSGKTVVGEFAVHLALEAGRKCFYTTPIKALSNQKYADLVRRYGPE
                     RVGLLTGDNTINGEAPVVVMTTEVLRNMLYAGSHTLLGLGFVVMDEVHYLSDRFRGAV
                     WEEVVIHLPESVQLVSLSATVSNAEEFGDWLVAVRGDTEVVVEEHRPVPLWQHVLTHD
                     GLHDLFLERADDPSSHGRREVNPELVRLARDEVRAERFGARRPHRGGQRPRHGARPPG
                     RAEVISRLDAEGLLPAITFIFSRAGCDAAVEACLRTGLRLTTPAEREQVRALVDARTA
                     TIPREDLPVLGYAGWRDGLERGIAAHHAGMLPTFKEVVEELFVRGLVKAVFATETLAL
                     GINMPARSVVLERLVKWNGETHAEITPGEYTQLTGRAGRRGIDVEGHAVVVWSPGFDP
                     RAVAGLASTRTYPLRSSFRPSYNMAVNLVGQLGRARGRELLETSFAQFQADRAVVGLA
                     RQVQRNEEALDGYRAAMTCHLGDFEEYAGLRHALKDREATLAREGVAQRRAAAAASLE
                     RLRPGDVIRVPTGRHAGLAVVLDPGFDGSREGPRPTVLTAERQVKRLSAVDFPTEVTP
                     LERLRLPRGFNPRNPQQRRDLASTLRARVPEGHRPSRRRSAAADDEEIARLRAAIRQH
                     PCHGCDEREDHARWAERYWRLRRETESLERRVSGRTNTVARTFDRVCAVLESLGYLAG
                     DQVSPDGRRLASLYGELDLLAAECLRAGLWEGLTPPELAAAVSALVFESRQPDDAATP
                     RVPGGRVRQVLTDTVHLWARLDELEQDHGLDFLREPDVGFAWAAHRWAGGARLDAVLA
                     EADLPAGDFVRWTKQVIDLLDQVADAAGPDSTVRATAHRAIVALRRGVVAYSSVG"
     gene            complement(5035..>5306)
                     /locus_tag="VLC50_00115"
     CDS             complement(5035..>5306)
                     /locus_tag="VLC50_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019884973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sphingosine kinase"
                     /protein_id="HSF25899.1"
                     /translation="CPASRIDDGYLDVTVVGPISKATLVRLFPRVYSGRHVEHPAVTT
                     YRARRVSLAAPGVTAYADGERIAPLPLTVTVVPGALRVLAPPPAG"
BASE COUNT          689 a         1985 c         1948 g          684 t
ORIGIN      
        1 taaggcagtc cgagcagccc cgcctcgccc aacgtccgga cgagctcgcg gggaaagcgc
       61 gactcggcct cgtgcaccgc cgcaaccggg gtcaacacgt cgcgggccag ctcgcgggtc
      121 agcccgatca gctcgctggc ctcgtcggtc ggcatgatcc gctcgacagg cacctcagcc
      181 ctccagcacg acgaggtcgc gggtgctgcg gttgagccgc tcgatcccgt cctcggtcac
      241 gacgacgatg tcctcgatcc gggcaccgtg gcggccctcg aggtagatgc ccggctcgac
      301 ggagaaggcc atccccggct cgagtggcag ggcgttgccg gccacgatgt agggctcctc
      361 gtgggtctcc accccgatgc cgtggccggt gcggtggaag aactccttgc cccagccggc
      421 gtcgttgatg accgtgcggg cggcagcgtc gacgctctcg gcgctgaccc cgggccggac
      481 gtgctcgcag gcggcggcct gggcggcctg cagcacggcg tagtagtcga cgaactccgc
      541 gggcggcgcg cccacggcgt aggtgcgggt ggagtcggag cagtagccgc ccatcgagcc
      601 gccgatgtcg atcaccaccg ggtcgccggt ctcgatgacc cggtcgctct gctcgtggtg
      661 cgggctggcg ccgttgggcc cggacccgac gatgacgaag tcgacagtgt catggccctc
      721 ggccaggatc gccgcggcga tgtcgcggcc cacctcccgc tcggtgcgcc cggcccgcag
      781 ccactccccg atccgggcgt gcacccggtc gatggccgca cccacggccc gcagcgcctc
      841 cacctcttcg ggggccttgc gcatccgcag gtcgcgcagc acgaccgaac ccagcacctg
      901 ctgcgcctgc ggcagcgcct cccggaaccg cagcaccttc tcggcccaca tctggtcgga
      961 cagcgcgacc gtggcgaccg cgccgagccg gtgcgtcagc accgccaccg ggtcgtcggt
     1021 ctcgtcccag gccaccactt ccaggtcgag cccgcgcacc ggcgaggcca gcgcggccga
     1081 gacctccaga cgaggtacga cgaggaacgc gtccccttgt gctggtacga cgaggcaggt
     1141 gagccgctcg agcgggatgg cgtcgtagcc ggtgagccag cgcaggtcgg gtccaggcgt
     1201 gacgagcagc gcgtcgacgc cggcctgtgt gcaggcggtg cgggctcggt cgatccggtc
     1261 ggggctgatc gcggtcgggg gcatctgtcc aatatatggg acggtgctgc gactctcgcg
     1321 cggcgcccac gggtgcggtc cggcatgcga cgatcttcgc gtggccaagg gaaccggtga
     1381 gcggctcatg ctgctcgaca ccgcgtcgct gtactttcgc gccttcttcg gggtgcccga
     1441 cagcctgcgt gcccccgacg gcacgccggt caacgcggtc cgcgggctgc tcgacttcat
     1501 cgcccggctg gtcaccgacc gggcgccgac ccggctggtg gcctgctggg acgacgactg
     1561 gcgcccggtg ttccgggtcg ccgcgatccc gtcgtacaag gcccaccggg tggccgacga
     1621 ggccgccaat gccgaggacg tgcccgacct gctctccccg caggtcccga tcatcgtcga
     1681 cgtgctcgcc gcgctgggca tcgcccgggt cggggcgcca gggcacgagg ccgacgacgt
     1741 catcggaacg ctggccaccc aggccacctg cccggtcgac gtcgtgaccg gtgaccgcga
     1801 cctgttccag ctggtcgacg acgccggcga ggtccgggtg ctctacaccg cccgcggcgt
     1861 gggcaagcat gacgtggtcg acgaggccga ggtgcgtcgc cgctacggcg tgccggcccg
     1921 ggcctacgcc gacttcgcca cgctgcgcgg cgacccctcc gacgggctgc ccggggtggc
     1981 cggggtcggc gagaagaccg ccgcctcact gttgacgacg tacggcgacc tgccgggggt
     2041 gctcgccgcg ctcgacgacc cgaagagcgg gctgagcccg accctgcgcc gccggctcga
     2101 cgcggcccgc gactacctgg cggtggcgcc ggcggtggta cgcgtggtca ccgacttgga
     2161 cctgcccgcg gtcgacgacc ggctgccgcg ggcgccggcc gacctcgagg ggctggccga
     2221 gctggccacc cgctggaacc tcggcggcag cgtccagcgg gtagtggccg ccctcgccgc
     2281 ctgaccagtc agccgacgga ggagtaggcg accacgccgc ggcgcagcgc gacgatcgcc
     2341 cggtgagcgg tggcacgcac tgtcgagtcg ggcccggccg cgtcggccac ctggtcgagc
     2401 aggtcgatga cctgcttggt ccagcgcacg aagtcgccgg ccggcaggtc ggcctcggcg
     2461 agcaccgcgt cgaggcgggc gccgccggcc catcggtgcg cggcccaggc gaagcccaca
     2521 tcgggctcgc gcaggaagtc gagcccgtgg tcctgctcga gctcgtcgag gcgggcccac
     2581 aggtgcaccg tgtcggtgag cacctgtcgg acccgcccgc cgggcacccg tggggtcgcg
     2641 gcgtcgtcgg gctggcgtga ctcgaacacc agcgccgaca cggccgcggc cagctccggc
     2701 ggggtcagcc cctcccacag cccggcccgc aggcactcgg cggccagcag gtcgagctcg
     2761 ccgtagagcg aggccagccg ccgcccgtcg gggctgacct ggtcgcccgc caggtagccc
     2821 agcgactcca gcaccgcgca cacccggtcg aacgtgcggg ccaccgtgtt ggtgcgtccg
     2881 gacacccgtc gctccagcga ctcggtctcc cggcgcagtc gccagtagcg ctcggcccag
     2941 cgggcgtggt cctcccgctc gtcgcagcca tggcaggggt gttgccggat cgccgcgcgc
     3001 agccgggcga tctcctcgtc atcggccgcg gccgagcgcc gccgcgacgg tcggtggccc
     3061 tcgggcaccc gggcacgcag cgtggaggcc aggtcgcggc gttgctgcgg gttgcgcggg
     3121 ttgaagccgc gcggcagccg cagccgctcg agcggggtca cctcggtcgg gaagtcgacg
     3181 gcggacagcc gcttcacctg acgctcggcg gtgagcacgg tggggcgggg gccttcccgg
     3241 ctgccgtcga aacccgggtc gagcaccacg gccagcccgg cgtggcggcc ggtcggcacc
     3301 ctgatcacgt cgcccgggcg cagccgctcc agcgacgcgg ccgcggcagc ccggcgctgg
     3361 gcgacgccct cgcgggccag cgtggcctcg cggtccttca gcgcgtggcg cagcccggcg
     3421 tactcctcga agtcacccag gtggcaggtc atcgcggcgc ggtagccgtc gagggcctcc
     3481 tcgttgcgct gcacctgccg ggccagtccc accaccgctc ggtcggcctg gaactgcgcg
     3541 aacgaggtct ccagcagctc gcgtccccgg gcccggccga gctggccgac gaggttgacg
     3601 gccatgttgt acgacggccg gaagctcgag cgcagcgggt aggtgcgtgt cgaggcgagc
     3661 ccggccacgg cgcgcgggtc gaagccgggc gaccagacga cgaccgcgtg gccctcgacg
     3721 tcgatgccgc gccggccggc ccgcccggtc agctgggtgt actcccccgg cgtgatctcg
     3781 gcgtgcgtct cgccgttcca cttcaccagc cgctcgagca cgaccgagcg tgccggcatg
     3841 ttgatgccca gcgccagcgt ctcggtggcg aagacggcct tgaccagccc gcggacgaac
     3901 agctcctcga cgacctcctt gaacgtcggc agcatcccgg cgtggtgcgc ggcgatgccc
     3961 cgctccagcc cgtcgcgcca gccggcgtag ccgagcaccg gcaggtcctc gcgcgggatc
     4021 gtcgcggtgc gcgcgtcgac gagggcgcgc acctgctcgc gctcggccgg ggtggtcagc
     4081 cgcagcccgg tgcgcaggca cgcctcgacc gccgcgtcac agccggcccg gctgaagatg
     4141 aacgtgatcg ccggcagcag cccctcggcg tcgagccggc tgatcacctc ggcgcggccg
     4201 ggcgggcgcg cgccgtgccg gggccgctgc ccgccccggt gcgggcggcg ggccccgaag
     4261 cgttcggcgc gcacctcgtc gcgggccagc cgcaccagct cggggttgac ctcgcgccgt
     4321 ccgtgcgacg acgggtcgtc ggctcgctcg aggaacaggt cgtgcagccc gtcgtgggtc
     4381 agcacgtgct gccacagcgg caccggccgg tgctcctcga cgaccacctc ggtgtcaccg
     4441 cgtacggcga ccagccagtc accgaactcc tcggcgttgc tcacggtcgc cgacagcgag
     4501 accagctgca ccgactccgg caggtggatg accacctctt cccagaccgc gccgcggaac
     4561 cggtcggaga ggtagtgcac ctcgtccatc acgacaaagc cgagcccgag caacgtgtgc
     4621 gagccggcgt agagcatgtt gcgcagcacc tcggtggtca tcacgaccac cggcgcctcg
     4681 ccgttgatgg tgttgtcgcc ggtgagcagc ccgacccgct ccggaccgta gcggcgcacc
     4741 aggtcggcgt acttctggtt ggacaacgcc ttgatcggcg tcgtgtagaa gcacttgcgc
     4801 ccggcctcga gagccaggtg tacggcgaac tcgccgacca ccgtcttgcc cgacccggtg
     4861 ggcgccgcga ccaggacgcc ctgcccggcc tccagcgcct cgcacgcgct gacctggaag
     4921 ccgtcgagcg ggaagtcgta gagcgcgcgg aaccggccca gtgcggaggc cgcctccgtg
     4981 cgacgccggc gggcggcggc gaaacgctcc gcgggcgagc tcatgacgcc gacgctaccc
     5041 ggccggcggc ggggcgagca cacgtagtgc gccgggcacc acggtgacgg tcagcggcag
     5101 cggggcgatc cgctcgccgt cggcgtacgc ggtgacaccg ggcgcggcca gcgagacccg
     5161 tcgagcccgg taggtggtca ccgccgggtg ctcgacgtgc cgcccggagt agacgcgtgg
     5221 gaacagccgc accagcgtgg ccttggagat cggcccgacc acggtgacgt cgaggtaccc
     5281 gtcgtcgatg cgcgacgcgg ggcaca
//
LOCUS       DATIBB010000006         9565 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_1054231, whole genome shotgun sequence.
ACCESSION   DATIBB010000006 DATIBB010000000
VERSION     DATIBB010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 9565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9565
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1054231"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..186)
                     /gene="ssrA"
                     /locus_tag="VLC50_00120"
     tmRNA           complement(<1..186)
                     /gene="ssrA"
                     /locus_tag="VLC50_00120"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(268..1869)
                     /locus_tag="VLC50_00125"
     CDS             complement(268..1869)
                     /locus_tag="VLC50_00125"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HSF25900.1"
                     /translation="MSSYDASDRDPVDEESVTGLSAPGRLRLLVAGALVVQLVALALL
                     GMGVSSAALAAQAVAIALVGVLAWLLARLVHELGGVWAFATAFARGDLTDVDAVSGSG
                     SLAGLAGALSAAAQRTRAVVQVVTDSAASLNASADQLAVSSGTIATTFAESTDQATVV
                     SASADQVSHNVSSVSTGAAELDSSVSEIARSVHEAARVAAEAVASAQSTTEVMTRLES
                     SSAEIGNVVKLITTIAEQTNLLALNATIEAARAGEAGKGFAVVASEVKDLAQETSRAT
                     EDISRRILAIQEDSRGAAGAIDGIREVIDRIAAHQTAIASAVEEQTATTAEMSRNLAE
                     AAAGSADIAVTVGGLATASQNALTGLARAKSAAAELAALAGGLRHVGASFTLPHPKII
                     EHEVGAEGGVALEVPGVVSVSHVPHLRTVVVRWLRYDDFAVKPALNKQFELIQRHRLR
                     SVIVDSQDAVGCYSPEMNRWIGQEFVPRMLSTEVRVLVTVVPRSALAGMANQDWQSGG
                     TQAGMQMVEVASMAEAERICREVAA"
     gene            2012..3943
                     /locus_tag="VLC50_00130"
     CDS             2012..3943
                     /locus_tag="VLC50_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016880077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HSF25901.1"
                     /translation="MAETSTAPRVSEQQSRAVAEAARETRWDQPSFAKELYLGRFRLD
                     LIHPHPRPSGPEEAPGLAFLDRLRRFCESECDGSVIEREDRIPDAVIKGLAALGVFGM
                     KIPEAYGGLGLSQLHYNKALQLLGTVHPSLGALISAHQSIGVPEPLKMFGSPAQKQAY
                     LPRCARGAISAFLLTEPDVGSDPARLQMTATPSPDGSSYVLTGSKLWTTNGVVAELLV
                     VMARVPGGPKGGITAFVVEADSPGITVEHRNAFMGLRGLENGVTRFDAVQVPAEQVIG
                     AVGDGLKIALTTLNTGRLSIPAMCTAAAKWSLKVAREWSRERVQWGRPIAEHAAVAHK
                     IGFIAATTYALESVLDLSAQLSDAGRTDIRIEAALAKLWSSEMAWRVADELVQIRGGR
                     GYETAESLAARGERAVPAEQMLRDLRINRIFEGSTEIMHLLIAREAVDTHLAVAGALI
                     DPSADVATKVRAGARAGGFYAGWLPRLAAGPGMLPTSYDEFGRLARHLRYVERASRRL
                     ARSTFYGMARWQGRLERKQGFLARVVDIGAELFAISAACVRAEMLRATDPAEGAAAVE
                     LADAFAAQSRLRVEALFRALWRNSDGSDERLARRTTADAFTWLEAGIVDPSGPGPWIA
                     STDPARPVADSVARRVLRT"
     gene            4108..4707
                     /locus_tag="VLC50_00135"
     CDS             4108..4707
                     /locus_tag="VLC50_00135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF25902.1"
                     /translation="MRISLASLVAAGVIGVAGITGLALVGPALVPANASPVEDEAPAH
                     HPVLRDRPADVKAALADLVSDGTLTQAQADAVAAKLREVGPRGPDGPHGPRGPRLRAG
                     GVTVALGVAAKALGITPGELRTALRQGDTLAEVAKAHGVDVPTLVDALVKAATTRVDA
                     AVADGRLTSARAEKIKAQLRERITDLVQEGLPMRGRSGL"
     gene            complement(4704..5189)
                     /gene="smpB"
                     /locus_tag="VLC50_00140"
     CDS             complement(4704..5189)
                     /gene="smpB"
                     /locus_tag="VLC50_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018502565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HSF25903.1"
                     /translation="MVKEKGRKLIAANRKARHDYTIESTYEAGVVLTGTEVKALRAGR
                     ASLVDGWATITDGEAWLHGVHIPEYTQGTWNNHEPRRPRKLLLHRHEIDRLIGKTKET
                     GHTLVPLALYFKDGRAKIEIALAKGKKSYDKRQAIAERDANRELAREMGRRIKGSRPP
                     R"
     gene            complement(5194..6378)
                     /locus_tag="VLC50_00145"
     CDS             complement(5194..6378)
                     /locus_tag="VLC50_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019629208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HSF25904.1"
                     /translation="MGRLVRATALTCAVLTAYGAGVLTGVVGSADPVVAPAAQVKASP
                     QGVIDEAADRIAAHAARQVDREVLEQAAVEGMLEALGDRWSSFYRPSDYASLQEALDG
                     RYSGVGIWLRPGEHDGSIVVGSVAPGSPAARAGILGGERVLAVDGHPVSGLQVAEVAA
                     MLRGDGGTNVELTLSAGTSSRVVALARTTFTTADVTVEHLSDSVLGLRVSAFTRGVGR
                     ALRNALAEDRAAYRGGIVLDLRGNPGGLLDEAVEVASAFLDGGPVVSFERRNQARTTM
                     DALGTGDTRTPLVVLVDQGTASAAEVVAGALQDRDRAVVVGTQTFGKGSVQEPTTLSD
                     GSAIELTVGRYFTPDGRVIDGVGLQPDVLVAPSAPPATAERRATEVLSGLMAALAPSG
                     RG"
     gene            complement(6471..7706)
                     /locus_tag="VLC50_00150"
     CDS             complement(6471..7706)
                     /locus_tag="VLC50_00150"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HSF25905.1"
                     /translation="MRARTTSISLTAILLAVLMVAGLATPGAADSTQSKKKKLDATIK
                     VLKQDLAETSASLRDAAIALARAKSALPDAQAAVTTAERGLAQAAARDAVLVQELSVA
                     VKQEDKAARELAAVGERIADQQDSLGALANAAYRSGGMSQLAVALEADSPDQFADRLV
                     LVQTAMRAGDAAVTELARAKADMRNTQARLEAKRAQVAAMREESRQLVEAKRKLSQQA
                     KAAKARVDQLIADRTKAMTAIAREKQAEQKRIAAMQAESSKLQQILRQRARASRSSGR
                     MVIGSGVLSRPVSGPITSSYGMRTHPITGIYKLHDGTDFGASCGTPIRAADDGTVVQA
                     AYRTGYGNQTVVDHGVVNGRSLATSYSHQSRFAARSGQHVSKGQVIGYVGSTGYSTGC
                     HLHFMVYVNGGVTNPMNWL"
     gene            complement(7833..8738)
                     /gene="ftsX"
                     /locus_tag="VLC50_00155"
     CDS             complement(7833..8738)
                     /gene="ftsX"
                     /locus_tag="VLC50_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009612694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HSF25906.1"
                     /translation="MRAQFILTEIGIGLRRNLTMTVAVVVTVAISLALFGTGLLIRQQ
                     VDTMKDYWYDRVEVAVFLCGEGAREPSCAAGEVTAEQRQQIEADLTSLPQVSKVYYES
                     KDEAYAHFREQFKDSAIFDTVRPESMPESFRVKLDDPEQFQVVRSAFEGRPGVQDVQD
                     QKQVLDKFFKVLNGFQLAALIVALVQIAAAALLISNTIRVAAFSRRRETGIMRLVGAS
                     NFYIQLPFILEGAIAGLIGAGFASAALIGMQKFVVEDRLVPGFPFTRFIGWDAVWSTV
                     PIMLLTGIILAGLASFVTLRRYLRV"
     gene            complement(8791..9480)
                     /gene="ftsE"
                     /locus_tag="VLC50_00160"
     CDS             complement(8791..9480)
                     /gene="ftsE"
                     /locus_tag="VLC50_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013116478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HSF25907.1"
                     /translation="MIRFDSVTKVYPNQVRPALDDVSVEIEKGEFVFLVGQSGSGKST
                     FLRLVLKEETVSSGSVYVAGKDLGRLSPWRVPSLRRQIGTVFQDFRLLPNKSVRENVA
                     FALEVLGKPRHHINTVVPEVLALVGLDGKEDRRPDELSGGEQQRVAVARAFVNRPMIL
                     IADEPTGNLDPTTSVGIMKLLDRINRTGTTIVMATHDKGIVDQMRKRVIELEGGHVVR
                     DQTRGVYGYSR"
BASE COUNT         1307 a         3415 c         3467 g         1376 t
ORIGIN      
        1 agacaccggg ccggaagcag tcccgggctg acagacatcg agtcccgctg tcaggcagcg
       61 agagcgacgt cagcgcgcga tgtattggcg cttaatcgtt tccagggcat ggtttacgag
      121 gtcatccctg ggatcctcgg cccgcttctc ttggatcgac gtccgaagtc gaaaccgttc
      181 acccccctgt gcagttgtca agcacgcccc gcaggacgca cctacgagac tacccactgc
      241 aacgaccggc gcgcacgtcg gattccgtca ggcggcgacc tcgcggcaga tccgctcggc
      301 ctcggccatc gaggcgacct cgaccatctg catcccggcc tgggtgccgc cgctctgcca
      361 gtcctggttg gccatgccgg ccagcgcact gcgcggtacg acggtgacga gcacgcgcac
      421 ctcggtggac agcatgcgcg gcacgaactc ctggccgatc cagcggttca tctccgggga
      481 gtagcagccg acggcgtcct gcgagtcgac gatcaccgag cgcagccggt ggcgctggat
      541 cagctcgaac tgcttgttga gcgccggctt gacggcgaag tcgtcgtagc gcagccaccg
      601 gacgacgacg gtgcgcaggt gcggcacgtg cgagacgctg accacgccgg gcacctcgag
      661 cgccacgccg ccctcggcac cgacctcgtg ctcgatgatc ttcgggtgcg gcagcgtaaa
      721 gctggccccg acgtggcgca gcccaccggc cagcgcggcc agctcggcgg cggcgctctt
      781 ggcccgggcc agcccggtca gcgcgttctg cgaggcggtg gccagcccgc cgacggtcac
      841 cgcgatgtcc gccgagccgg cggcggcctc ggccaggttg cggctcatct cggcggtcgt
      901 cgcggtctgc tcctcaaccg cgctggcgat cgcggtctgg tgtgcggcga tccggtcgat
      961 cacctcacgg atcccgtcga tcgccccggc cgcgccgcgg ctgtcctcct ggatcgccag
     1021 gatccggcgg ctgatgtctt cggtggcccg cgacgtctcc tgcgccaggt ccttgacctc
     1081 gctggccacc acggcgaacc ccttgccggc ctcgccggcc cgggccgcct cgatggtggc
     1141 gttgagcgcg agcaggttgg tctgctcggc gatcgtcgtg atcagcttga ccacgttgcc
     1201 gatctcggcc gaggaggact ccagccgggt catcacctcg gtggtcgact gggccgaggc
     1261 caccgcctcg gcggccaccc gcgccgcctc gtgcacgctg cgagcgatct cggagaccga
     1321 gctgtcgagc tcggcggcgc cggtcgagac cgacgagacg ttgtgcgaaa cctggtcggc
     1381 cgaggcgctg accacggtgg cctggtcggt ggactcggcg aaggtcgtgg cgatcgtgcc
     1441 gctggagacg gccagctggt cggccgaggc gttgagcgag gcggccgagt cggtgaccac
     1501 ctgcacgacc gcgcgggtgc gctgggcggc cgcgctcagc gcaccggcca gcccggcgag
     1561 gctgccgctg ccggagacgg cgtcgacgtc ggtgaggtcg ccgcgggcga acgccgtggc
     1621 aaaggcccac acgccgccca gctcgtgcac cagccgggcg agcaaccagg ccagcacacc
     1681 gaccagcgcg atggccaccg cctgcgcggc gagcgccgcg ctcgagacgc ccatgcccag
     1741 cagggcgagc gccaccagct ggaccaccaa cgcgcccgcg acgagcagcc gcaaccggcc
     1801 gggcgcggac aggcccgtga cggactcctc gtcgaccggg tcgcggtcag aggcgtcgta
     1861 cgacgacatc gcatctcctg atgatgaggt gactgcatga tcaccatcgg cgccggaaac
     1921 gggcatctga ggcaagcccg gcgaccgact tgccttctgt ctactggcga gtaacagtcg
     1981 aggcccacac accggcaccg gggaggccgt cgtggccgag accagcactg cgccgcgggt
     2041 gagcgagcag cagtcacgcg cggtcgccga ggccgcccgc gagacccgtt gggaccagcc
     2101 ctcgttcgcc aaggagctct acctgggccg gttccggctc gacctgatcc acccgcaccc
     2161 ccgtccgagc gggcccgagg aggccccggg gctggccttc ctcgaccggc tacgccgctt
     2221 ctgcgagtcc gagtgcgacg ggtcggtgat cgagcgagag gaccgcatcc ccgacgcggt
     2281 catcaaggga ctggccgcgc tcggcgtgtt cgggatgaag atccccgagg cgtacggcgg
     2341 gctcgggctg tcccagctgc actacaacaa ggcgctgcag ctgctcggca ccgtgcaccc
     2401 gagcctgggc gcgctgatct cggcccacca gtcgatcggc gtccccgagc cgctcaagat
     2461 gttcggctcg ccggcgcaga agcaggccta cctgccccgg tgcgcccgcg gcgcgatcag
     2521 cgcgttcctg ctcaccgagc ccgacgtcgg ctccgacccg gcccggctgc agatgaccgc
     2581 gacgccgagc cccgacgggt cgtcgtacgt cctgaccggc tccaagctgt ggaccaccaa
     2641 cggggtggtc gccgagctgc tggtggtgat ggcccgggtg cccggcgggc ccaagggcgg
     2701 gatcaccgcg ttcgtcgtgg aggccgactc ccccggcatc accgtcgagc accgcaacgc
     2761 gttcatgggg ctgcgcgggc tggagaacgg cgtgacccgc ttcgacgccg tgcaggtgcc
     2821 ggccgagcag gtcatcggcg cggtcggcga cgggctcaag atcgcgctga ccacgctcaa
     2881 cacgggccgg ctgtcgatcc cggcgatgtg cacggcggcg gcgaagtggt cgctcaaggt
     2941 ggcccgcgag tggagccgcg agcgggtgca gtggggtcgc ccgatcgccg agcacgccgc
     3001 ggtcgcccac aagatcggct ttatcgcggc cacgacctat gcgctcgagt cggtgctcga
     3061 cctgtcggcg cagctgtccg acgcgggccg caccgacatc cggatcgagg cggcgctggc
     3121 caagctgtgg tcgagcgaga tggcctggcg ggtcgccgac gagctggtgc agatccgggg
     3181 cgggcgcggc tacgagaccg ccgagtcgct ggccgcacgc ggagagcggg cggtgccggc
     3241 cgagcagatg ctgcgtgacc tgcggatcaa ccggatcttc gagggctcca ccgagatcat
     3301 gcacctgctc atcgcccggg aggccgtcga cacccacctc gcggtggccg gcgcgctcat
     3361 cgacccgtcg gctgacgtcg cgaccaaggt gcgggccggc gcgcgggccg gcgggttcta
     3421 cgcgggctgg ctgccccggc tggccgcggg accggggatg ctgcccacgt cgtacgacga
     3481 gttcggccgg ctcgcccggc acctgcgcta cgtcgagcgc gcctcccgcc ggctggcccg
     3541 ctcgacgttc tacgggatgg cccgctggca gggtcggctc gagcgcaagc agggcttcct
     3601 ggcccgggtc gtcgacatcg gcgccgagct gtttgccatc tcggcggcct gcgtgcgggc
     3661 cgagatgctg cgcgcgaccg acccggccga gggggcggcg gccgtcgagc tggccgacgc
     3721 gttcgccgcg cagtcgcggc tgcgggtcga ggcgctgttc cgcgcgttgt ggcgcaacag
     3781 cgacggctcc gacgagcggc tggcccgtcg tacgacggcc gatgcgttca cctggttgga
     3841 ggccggcatc gtcgacccgt ccgggcccgg cccgtggatc gcctcgactg acccggcccg
     3901 tcccgtcgcc gactcggtgg cccgccgggt cctgcggacc tgaccctccc gccgtgatca
     3961 tgcacgagcg cggcgcagag ctgcatgatc acgacgggga tggggattga tcggcatttc
     4021 acagggtgtt ctcaggctgc gttcagggtc gggccgcacc atggagccac cccgggagcg
     4081 gcccgggcct caccgagagg acccgacatg cggatcagtc tggcgagcct ggtcgccgcg
     4141 ggggtgatcg gcgtcgccgg catcaccggg ctggccctgg tcggccccgc gctggtgccg
     4201 gccaacgcga gcccggtcga ggacgaggcg cccgcgcacc accccgtgct gcgggatcgc
     4261 cccgccgatg tcaaggccgc gctggccgac ctggtcagcg acggcaccct cacccaggcg
     4321 caggccgacg ccgtcgccgc gaagctgcgc gaggtcggcc cccggggccc agacggtccg
     4381 cacggcccgc gcggcccgcg cctgcgcgcc ggcggggtca cggtcgcgct gggggtggcg
     4441 gccaaggcgc tgggcatcac gcccggcgag ctgcgcaccg cgctgcgcca gggcgacacg
     4501 ctcgccgagg tggccaaggc ccacggcgtc gatgtcccca cgctggtcga cgccctggtc
     4561 aaggccgcga ccacgcgcgt cgacgcggcc gtggccgatg gcaggctcac gagcgcccgc
     4621 gccgagaaga tcaaggcaca gctgcgcgag cggatcaccg acctcgtgca ggagggcctg
     4681 ccgatgcggg gtcgctcggg gctctaacgc ggcgggcgcg agcccttgat ccgccggccc
     4741 atctcgcggg ccagctcgcg gttggcgtcg cgctcggcga tcgcctgacg cttgtcatag
     4801 ctcttcttgc ccttggccag cgcgatctcg atcttggccc gcccgtcctt gaagtagagc
     4861 gcgagcggca ccagggtgtg cccggtctcc ttggtcttgc cgatcagccg gtcgatctcg
     4921 tgccggtgca gcagcagctt gcgcggccga cggggctcgt ggttgttcca ggtgccctgg
     4981 gtgtattcgg ggatgtgcac cccgtgcagc caggcctcgc cgtcggtgat cgtggcccag
     5041 ccgtcgacca gcgacgcgcg cccggcacgg agcgccttga cctcggtgcc ggtcagcacg
     5101 acgccggcct cgtaggtcga ctcgatcgtg tagtcatggc gcgccttgcg gttggccgcg
     5161 atcagcttgc ggcccttctc cttgaccatc gcgtcagccc cgcccgctgg gtgccagggc
     5221 ggccatcagc ccggagagca cctcggtggc ccgccgctcg gcggtggccg gcggcgccga
     5281 cggggcgacc aggacgtcgg gctgcagccc gaccccgtcg atgacccgcc cgtcgggggt
     5341 gaagtagcgg cccacggtca gctcgatcgc gctgccgtcg gacagcgtgg tcggctcctg
     5401 gaccgagccc ttgccaaacg tctgggtgcc gaccacgacc gcccggtcac ggtcttgcag
     5461 cgcaccggcg acgacttcgg cggcgctggc ggtgccctgg tcgacgagca ccacgagcgg
     5521 ggtccgggtg tcgccggtgc cgagcgcgtc catcgtcgta cgtgcctggt tgcgccgctc
     5581 gaaggagacg accgggccgc cgtcgaggaa cgccgaggcg acctcaacgg cctcgtcgag
     5641 cagcccgccc gggttgccgc gcagatcgag gacgatgccg ccgcgatagg cggcacggtc
     5701 ttcggccagc gcgttgcgca gcgcccgccc gaccccgcgg gtgaacgcgc tcacgcgcag
     5761 ccccagcacg gagtcgctca ggtgctcgac ggtcacgtcg gcggtggtga acgtcgtgcg
     5821 agccagcgcg accacccgcg acgaggtgcc ggccgacagg gtgagctcga cgttggtgcc
     5881 accgtcgccc cgcagcatgg ccgccacctc ggcgacctgc agcccgctga ccgggtgccc
     5941 gtcgacggcc aggacccgct cgccgccgag gatgccggcc cgggcggcgg gcgagccggg
     6001 cgccacgctg cccacgacga tcgagccgtc gtgctcgccc gggcgcagcc agatgcccac
     6061 cccgctgtag cgcccgtcga gcgcctcctg cagcgacgcg tagtcgctcg ggcggtagaa
     6121 gctcgaccac cggtcaccca gcgcctcgag catgccctcg acggcggcct gctcgagcac
     6181 ctcccggtcc acctggcggg ccgcgtgggc ggcgatccgg tcggcggcct cgtcgatgac
     6241 cccctggggg ctggccttga cctgggcggc cggcgccacg acggggtcgg cgctgcccac
     6301 cacgccggtg agcacgccgg cgccgtacgc cgtaagcacg gcgcacgtca acgcggtggc
     6361 gcgcaccagt cggcccatga cctgggattc tacggaccga gcgccgacac cgccgcgcag
     6421 aatccacaga tcgggcagta cggggctaac cgggacctgt cagagccagc tcagagccag
     6481 ttcatgggat tggtgacgcc gccgttgacg tagaccatga agtgcaggtg gcagccggtc
     6541 gagtagccgg tgcttccgac gtagccgatc acctgaccct tgctcacgtg ctggcccgag
     6601 cgcgccgcga accgggactg gtggctgtag gaggtggcca gcgagcggcc gttgaccacg
     6661 ccgtggtcga cgacggtctg gttgccgtag ccggtgcggt atgccgcctg caccacggtg
     6721 ccgtcgtcgg cggcccggat cggcgtgccg caggaggccc cgaagtcggt gccgtcgtgc
     6781 aacttgtaga tgccggtgat cgggtgcgtg cgcatgccgt acgacgaggt gatcggaccg
     6841 ctgaccgggc gcgagagcac cccgctgccg atcaccatgc ggccggacga gcggctggcc
     6901 cgggcgcgct ggcgcaggat ctgctgcagc ttgctcgact cggcctgcat cgcggcgatg
     6961 cgcttctgct cggcctgctt ctcccgggcg atcgcggtca tcgccttggt gcggtcggcg
     7021 atcagctggt cgacgcgggc cttggccgcc ttggcctgct ggctgagctt gcgcttggcc
     7081 tcgacgagct ggcggctctc ctcgcgcatc gcggcgacct gggcccgctt ggcctcgagc
     7141 cgggcctggg tgttgcgcat gtcggccttg gcccgggcca gctcggtgac cgcggcgtcg
     7201 ccggcgcgca tcgcggtctg cacgagcacg agccggtcgg cgaactggtc ggggctgtcg
     7261 gcctcgaggg cgaccgccag ctgactcatg ccgcccgagc ggtaggccgc gttggccagg
     7321 gcgccgagcg agtcctgctg gtcggcgatc cgctcgccca ccgcggccag ctcacgggcc
     7381 gccttgtcct cctgcttgac cgcgacggag agctcctgca cgagcacggc gtcgcgagcc
     7441 gccgcctggg ccagcccgcg ctcggcggtg gtcaccgcgg cctgcgcgtc gggcagcgcc
     7501 gacttggccc gggccagcgc gatcgccgcg tcgcgcagcg aggccgaggt ctcggccaga
     7561 tcctgcttga ggaccttgat ggtcgcgtcg agcttcttct tcttgctctg ggtgctgtcg
     7621 gcagcaccgg gagtagccag gcccgccacc atgagcaccg cgagcaggat cgcggtcagg
     7681 ctgatgctgg tggtgcgggc acgcataggc gacgggctcc tcagggtcgg ggccaccgcg
     7741 cgcatgtata gcacacgaaa tgtcgctatt tcctctgcca ctccgggcgc acgggtggcc
     7801 ggcgccgcgg gcggcgctgg cgcgggagct gctcagaccc gcaggtagcg acggagcgtg
     7861 acgaaggagg ccagcccggc caggatgatc ccggtcagca gcatgatcgg cacggtggac
     7921 cacaccgcgt cccagccgat gaaccgggtg aacgggaagc cgggcaccag ccggtcctcg
     7981 accacgaact tctgcatgcc gatcagcgcc gcgctggcga agccggcccc gatcagcccg
     8041 gcgatcgcgc cctcgaggat gaacggcagc tggatgtaga agttgctcgc cccgaccagg
     8101 cgcatgatgc cggtctcccg gcgtcggctg aacgcggcca cccggatcgt gttggagatc
     8161 agcagcgcgg ccgcggcgat ctgcaccagc gcgacgatga gcgcggcgag ctggaagccg
     8221 ttgagcacct tgaagaactt gtcgagcacc tgcttctggt cctggacgtc ctgcactccc
     8281 ggtcggccct cgaaggcgct gcggaccacc tggaactgct cggggtcgtc gagcttgacc
     8341 cggaacgact ccggcatcga ctccgggcgc accgtgtcga agatcgccga gtccttgaac
     8401 tgctcgcgga agtgcgcgta ggcctcgtcc ttggactcgt agtagacctt gctcacctgg
     8461 ggcagcgagg tgaggtcggc ctcgatctgc tggcgctgct cggcggtcac ctcgccggcc
     8521 gcgcagctgg gctctcgggc gccctccccg cagaggaaga ccgcgacctc gacccggtcg
     8581 taccagtagt ccttcatggt gtcgacctgc tggcggatga gcagaccggt gccaaacagc
     8641 gccagcgaga tggccacggt gacgaccacg gcgacggtca tcgtgaggtt ccgccgcagg
     8701 ccgatgccga tctcggtcag gatgaactgc gcgcgcatgg cttccttggg gtcgggtgcg
     8761 ccgggtctcg gtggcggggc tcggggcggg tcagcgggag tatccgtaga cgccgcgggt
     8821 ctggtcacgt acgacgtgcc cgccctcgag ctcgatcacc cgtttgcgca tctggtcgac
     8881 gatccccttg tcgtgcgtgg ccatcacgat cgtcgtaccg gtgcggttga tccggtcgag
     8941 cagcttcatg atgccgacgc tggtggtcgg gtcgaggttg ccggtcggct cgtcggcgat
     9001 gaggatcatc gggcggttga cgaacgcccg ggccaccgcc acccgctgct gctcgccgcc
     9061 ggagagctcg tcgggacggc ggtcctcctt gccgtccagg ccgaccagcg cgagcacctc
     9121 cggcaccacg gtgttgatgt ggtggcgcgg cttgccgagc acctcgagcg cgaacgcgac
     9181 gttttcccgc accgacttgt tgggcagcag ccggaagtcc tggaacacgg tgccgatctg
     9241 ccggcgcagc gaggggaccc gccacggcga gagccggccg aggtccttgc ccgcgacgta
     9301 gaccgagccg gagctgaccg tctcctcctt gagcacgagc cgcaggaacg tcgacttgcc
     9361 cgacccggac tgccccacca ggaagacgaa ctcgcccttc tcgatctcca ccgagacgtc
     9421 gtcgagcgcc gggcggacct ggttggggta gaccttggtg acggagtcga atcggatcac
     9481 ggcggcatca tgactggccc gggtagacga caggacggag cacgtacgac gacgcggcgg
     9541 gccaaccgcg gcgagtgtac gtgac
//
LOCUS       DATIBB010000007        11824 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_1712587, whole genome shotgun sequence.
ACCESSION   DATIBB010000007 DATIBB010000000
VERSION     DATIBB010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 11824)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11824)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11824
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1712587"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..284
                     /locus_tag="VLC50_00165"
     CDS             <1..284
                     /locus_tag="VLC50_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012919075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HSF25908.1"
                     /translation="ETSRLLTYKAAWLKDSGASRARLKQAAAIAKLHATESAVTATRI
                     ATQVFGGYGFMEEYPVARFYRDAKVLEIGEGTSEVQRMLIARGLGLPVE"
     gene            312..1823
                     /locus_tag="VLC50_00170"
     CDS             312..1823
                     /locus_tag="VLC50_00170"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020392131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HSF25909.1"
                     /translation="MEITEARAATLAPSPKGAAKLAKQRKLYVRERIDLLFDAGTFVE
                     DGQLANALATGLPADGVVTGRGLVEGRPAIVVANDPTVKAGSWGARTVEKMVRATEAA
                     LRDELPIFWFVDSAGARITDQVELFPGRRGAGRIFHNQVALSGKVPQICCLFGPSAAG
                     GAYIPAFCDLVVMVEGNASMYLGSPRMAEMVVGEKVTLEEMGGARMHCTVSGCGDNLA
                     VDDADAIEAAKQFFSYLPTCWREAPPEVEPTAPARGFDRDLVPARESQPFDVHEAIDA
                     LVDDGSFFEVKPLFAAELVVGLARIDGRPVGVLANNSAVKGGVLFTDSADKAARFIWL
                     CDAFNVPLVYLADVPGFMIGSEVERTGIIRHGAKMITAVAEATVPQVSVILRKAYGAG
                     LYAMAGPGFGPDACIALPTAKIAVMGPEAAVNAVFANKIAEIADEAERAAYVAARRAE
                     YEADVDLLRLASDLVIDGIVEPGELRGELVRRLALAAGRSRHFSARRHGVPPV"
     gene            1829..2029
                     /locus_tag="VLC50_00175"
     CDS             1829..2029
                     /locus_tag="VLC50_00175"
                     /inference="COORDINATES: protein motif:HMM:NF025192.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004658 - propionyl-CoA carboxylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase epsilon subunit"
                     /protein_id="HSF25910.1"
                     /translation="MSEPLHVRGGPSPDELAAALAVLAARRAPGDGSASATGFAAVWR
                     SVRRRALARSDQRHRPGANRSA"
     gene            2088..3227
                     /gene="efeO"
                     /locus_tag="VLC50_00180"
     CDS             2088..3227
                     /gene="efeO"
                     /locus_tag="VLC50_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007263807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HSF25911.1"
                     /translation="MTALGRASALALGVLMIGSLAACSQDPDEAAAAGTEVSVHATDT
                     ACELGAEEIEAGSATFAIHNEGSKVTEVYVLRSDESIVAERENIGPGTSADLTVALRA
                     GDYVVRCKPGMAGEGIAAPLAVTGGPASAAPAADARLEQAVTDYRAYVLAQVDDSIKL
                     TEALVAAVKAGNVAEAKRLYAPSRVGWESIEPVAESFGDIDPRVDLREADLAAGEEWT
                     GWHRIEKGLFVSGSTDGLASYGDQLLADLRELRQRVPDAEITPTSMANGAKELLDEVA
                     TGKVTGEEEAFSHTDLVDFEANVVGAEKVLVLLHEVVTDNEPELAATLDKEFGVVLGL
                     LDKHRDGDGFVSYDTVGDDDRRVLSDAVNALAEPLSNLAAAVTPR"
     gene            3224..4492
                     /gene="efeB"
                     /locus_tag="VLC50_00185"
     CDS             3224..4492
                     /gene="efeB"
                     /locus_tag="VLC50_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013474113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0033212 - iron import into cell [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter
                     deferrochelatase/peroxidase subunit"
                     /protein_id="HSF25912.1"
                     /translation="MTGPVLSRRRLLGVAGVGLVGAAAGVGGASLVERSGEDTGDRSS
                     LAAAGSATAVVFHGAHQAGIATPVQDRLQITAFDLTTDSRDELVALLREWTRAAEAMT
                     AGRDVGRGAVDGRAEAPPDDTGEALGLPPSRLTLTVGFGPSLFERDGVDRFGLRDARP
                     GALIDLPRFRNDALEPARCGGDIVVQACADDPQVAVHAIRNLARIGFGRAIVRWSQLG
                     FGKTSSTTSDEVTPRNLFGFKDGTNNITGAQHDRLDRHVWVGDEAAGPATWMRGGTYL
                     VARRIGMRIETWDRESLAGQEQMVGRTKREGAPLGQTHERDAVDLSALPADSHVALAH
                     PDANGGHAILRRGYSYVDGSDGLGRLDAGLFFIAFCRDAGVQYVPMQERMARLDTMAE
                     YLVHTGSGLFACPPGVDGPDDYWGRALFEA"
     gene            4579..5019
                     /locus_tag="VLC50_00190"
     CDS             4579..5019
                     /locus_tag="VLC50_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012687530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HSF25913.1"
                     /translation="MSYVRRFDHVGVTVADLDVVTAFFVALGLEVEGRGFVEGQFLDT
                     VCGIPDSRTEIVMLRSPGEGARLELARFVRPDSVPGSPTAMANELGLRNVAFEVNDLQ
                     AAVDWAASEGYGLVGGIGAYEDAWRMAYVRGPEGIIVALAERIG"
     gene            complement(5068..6468)
                     /locus_tag="VLC50_00195"
     CDS             complement(5068..6468)
                     /locus_tag="VLC50_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013827044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF25914.1"
                     /translation="MAERAARMVELAHRRLDPVGEGVHSRWREQVNEFCVDEIALALH
                     ATRRTAGVMLSFATDLDELPATRSALWEGRIDQGRARVLVNGLAGQPATTARAVEAAV
                     LPEAGELTSGQLRVAVSKALAHVRAAALDRRRTKAQAERRLQLWPDPASGTGTIHAAG
                     LEGADALAVWSACDAWARARGADDPRGMDARRADALVALVCGHPALGAPASDVPAGSR
                     DATSGAGGAFRRQVRLELAITLDALTGKSLTPAWLSSHGWITPAHARALIRDAETTTA
                     VAVTDPTTGALLTLGPDQTSPLPLTDLPHDPEPGDDGKDPDPDDDRPPDDDRGGPGGG
                     PPDRGGGGGGGSTGGGDSPSGGGGADADDGPGGEWFEDPGPYVIPAAMARLVRARDRV
                     CRFPGCRVPASRCDIDHTRAWPGGPTHPRNLACLCRTHHRLKQSRRWRVARHGPEGLT
                     WTTPTGHTRTRAPTRR"
     gene            complement(6472..7104)
                     /locus_tag="VLC50_00200"
     CDS             complement(6472..7104)
                     /locus_tag="VLC50_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020576836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HSF25915.1"
                     /translation="MPRLVLASASPARLATLRAAGVEPVVRVSDVDEDAVTAAAGAES
                     ATDIARLLARAKAEAVAATDGLPPAAIIVGCDSVLEINGRAYGKPASAAEAIDRWQRM
                     SGRRGTLHTGHHLVDLGSGRTVSGLASTVVRFAEVDDEEIEAYVATGEPLHVAGAFTI
                     DGLGGPFIDGIEGDPHNVVGISLPLMRCLLHELGHRFIDLWQTESVAGPL"
     gene            complement(7198..7794)
                     /locus_tag="VLC50_00205"
     CDS             complement(7198..7794)
                     /locus_tag="VLC50_00205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF25916.1"
                     /translation="MNETTRSILDEMLPHYDVTVDETLVVDSDVPSTYRAAHDLDFLS
                     VRSPLLSASMWVRGLPAKVRGNAPTIPRQLRLSDEGLDLPGWTLLGERPDEEIAFGAV
                     GRFWQRDITWHDVAREEFADFSEPGWGKIAASFSVRPYGEWRSLLTYGCRTATTDAES
                     RRRFGRYWMVIRPFVRHIMNATLHTIADDALRPTAVQI"
     gene            complement(7853..8071)
                     /locus_tag="VLC50_00210"
     CDS             complement(7853..8071)
                     /locus_tag="VLC50_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013222857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit epsilon"
                     /protein_id="HSF25917.1"
                     /translation="MSVALDPPLLRVLRGEPDADQLAALLAVVTSRGASGAPHRPAPA
                     STWADPARRLRPPMRPGPGAWRRSGLPR"
     gene            complement(8068..9675)
                     /locus_tag="VLC50_00215"
     CDS             complement(8068..9675)
                     /locus_tag="VLC50_00215"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007927389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HSF25918.1"
                     /translation="MADAMGGTEVPADIDIHTTAGKLADLRRRVGEARRAGSARAIEK
                     QHAKGKKTARERIALLLDPGSFVEMDELARHRTTNFGMEAVRPYGDGVVTGYGTVDGR
                     PVCVFAQDFTVFGGSLGEVFGEKIVKVMDLAMKIGAPMVGINDSGGARIQEGVVSLGL
                     YGEIFQRNVLASGVVPQISLIMGPCAGGAVYSPAITDFTVMVDEKSYMFITGPDVIKT
                     VTGEDVSQEELGGGRTHNTKSGNAHYLAADEDDAIEFAKALLAFLPSNNLEEPPAYDD
                     VEAELEVTDVDLELDTFVPDSANQPYDMRTVIEHVVDDGEFVEVQALFAPNIVVGFGR
                     VEGRPVGVVANQPMAFAGTLDIDASEKAARFVRTCDAFNVPILTFVDVPGFLPGTDQE
                     WNGIIRRGAKLLFAYAEATVPTVTVITRKAYGGAYDVMGSKHLGADINLAWPTAQIAV
                     MGAQGAVNILYRKELGGEEGSEARRRELIADYEDTLANPYIAAERGYVDAVIAPSQTR
                     VSIVRALRSLRTKRASLPPKKHGNIPL"
     gene            9717..10559
                     /locus_tag="VLC50_00220"
     CDS             9717..10559
                     /locus_tag="VLC50_00220"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016189199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HSF25919.1"
                     /translation="MPGSRWSDLERPPLDAQALRRALLAPSGPWTSLEVLATTGSTNA
                     DLVARARAGAPAGAVLVAEEQTAGQGRLDRRWVTPPRAALAVSMLLRPPVPARRWGWL
                     SLLVGLAATDAVRRVAELDAVLKWPNDVLVGERKLAGVLIERVDAPPGPAAVVGIGLN
                     VSLAEDELPVPEATSLRVAGAEVIDRDPLLRALLRAVAGRYEAWVGADGDATACGLRA
                     AYVDRCMTLGRPVTVHLPAGRREGGVAGSVDETGALVVRRDDGTDLTVTAGDVVHVRP
                     GAPD"
     gene            10622..11158
                     /locus_tag="VLC50_00225"
     CDS             10622..11158
                     /locus_tag="VLC50_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020576847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HSF25920.1"
                     /translation="MGIPQRLLSDDERLVLVLRPHWKEIIPAALVLLVTAPAASFLAA
                     ITPEGGAQGWLRLAVLVLAVLVLLRWSLWPFLTWLTTTYGVTSERIITREGVIARQGH
                     DMPLSRVNDVSFSHSAVERVLRCGTLVVESGGERGQLVLRDVPRVERVQREIYRLAEA
                     DELRRRGRGDEGAVLGDR"
     gene            11174..>11824
                     /locus_tag="VLC50_00230"
     CDS             11174..>11824
                     /locus_tag="VLC50_00230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HSF25921.1"
                     /translation="MDDLDPAELEEMLLEGPRLYRRAQVARYADSDLGQLTKLWRALG
                     FPDVPEGSAAFTDADLVALRRVLRLARAGVFDEDFIIGVTRAMGYHLARLVEWEYVAL
                     AEHVATRDGAGSEDAGPGGLALLTEHLVDFEALLLYVWRRQLVATAGRVLGGADEEVA
                     RRRRTVGFADLVSYTQLSQRLEARELAALVDRFEALSADIIAEHGGRLVKTVGDEVL"
BASE COUNT         1568 a         4147 c         4387 g         1722 t
ORIGIN      
        1 tggagacgtc gcggctgctc acctacaaag ccgcctggct caaggactcc ggcgcatcgc
       61 gcgcgcgact caagcaggcc gcggcgatcg ccaagctgca cgccaccgag tcggcggtca
      121 ccgccacccg catcgccacc caggtgttcg gcggctacgg gttcatggag gagtacccgg
      181 tggcgcgctt ctaccgcgac gccaaggtgc tcgagatcgg cgagggcacg agcgaggtgc
      241 agcggatgct catcgctcgg ggcctcgggc tgccggtcga gtagggtccg gctgtgtctc
      301 gaggcgagcc cgtggagatc accgaagccc gggcggccac cctcgcaccc tcccctaagg
      361 gcgcggccaa gctggccaag cagcgcaagc tctacgtccg cgagcgcatc gacctgctct
      421 tcgacgcggg cacgttcgtc gaggacggcc agctggccaa cgcgctggcc accgggctgc
      481 ccgccgacgg cgtggtgacc ggacgcgggc tggtcgaggg ccggcccgcg atcgtggtgg
      541 ccaacgaccc cacggtcaag gccggctcgt ggggcgcccg caccgtcgag aagatggtgc
      601 gggccaccga ggccgcgctg cgtgacgagc tgccgatctt ctggttcgtc gactccgcgg
      661 gtgcgcggat caccgaccag gtcgagctct ttcccggccg gcggggggcc gggcgcatct
      721 tccacaacca ggtggcgctg tccggcaagg tgcctcagat ctgctgcctg ttcgggccgt
      781 ccgccgccgg tggggcctac atcccggcgt tctgcgacct cgtcgtgatg gtcgagggca
      841 atgcgtcgat gtacctcggc agcccgcgga tggccgagat ggtggtcggc gagaaggtca
      901 cgctcgagga gatgggcggg gcccgcatgc actgcaccgt ctccgggtgc ggtgacaacc
      961 tcgcggtcga cgatgccgac gcgatcgagg ccgccaagca gttcttctcc tacctgccga
     1021 cctgctggcg cgaggcgccg cccgaggtgg agccgaccgc cccggcccgc ggcttcgacc
     1081 gcgacctcgt gccggcccgg gagtcgcagc ccttcgacgt gcacgaggcg atcgacgcgc
     1141 tggtcgacga cgggtcgttc ttcgaggtca agccgctgtt tgcggccgag ctggtggtcg
     1201 gcctggcccg catcgacggg cgcccggtcg gcgtgctggc caacaacagc gcggtcaagg
     1261 gcggggtgct gttcaccgac tcggccgaca aggcggcccg gttcatctgg ctgtgcgacg
     1321 cgttcaacgt gccgctggtc tacctggccg acgtacccgg attcatgatc ggctcggagg
     1381 tcgagcgcac cgggatcatc cgccacggcg ccaagatgat caccgcggtg gccgaggcga
     1441 cggtgccgca ggtgtcggtg atcctgcgca aggcgtacgg cgccgggctg tatgccatgg
     1501 cggggcccgg ctttgggcct gacgcctgca tcgcgctgcc gaccgccaag atcgccgtga
     1561 tggggccgga ggcggcggtc aacgcggtct tcgccaacaa gatcgccgag atcgccgacg
     1621 aggccgagcg ggcggcctac gtcgcggccc ggcgggccga gtacgaggcc gacgtcgacc
     1681 tgctccggct ggcctccgac ctggtcatcg acggcatcgt cgagcctggc gagctgcgcg
     1741 gcgagctggt ccgccggctg gctctggccg ccggtcgctc acggcacttc tccgcccgcc
     1801 ggcacggcgt gccaccggtg tgagcaccgt gagcgagccg ctgcacgtgc gcggcggccc
     1861 gtcgccggac gagctggccg ctgccctggc cgtgctcgcc gcccgccgcg ccccaggcga
     1921 tggatcggcg tcggccaccg gcttcgccgc ggtgtggcgg tcggtgcggc gtcgcgcgct
     1981 cgcgcgcagt gatcaacgtc accgccccgg tgccaaccgc tcggcttgac agtgcgtccg
     2041 atcttctact taggttaggc ggccctaact agaggagatc ggaaaacgtg acagccctcg
     2101 gccgtgcctc ggcccttgcc ctcggcgtac tcatgatcgg cagccttgct gcctgcagcc
     2161 aggatcccga cgaggccgcg gcggcgggga ccgaggtcag cgtgcacgcg accgacaccg
     2221 cctgcgagct cggggccgag gagatcgagg ccgggtcggc cacgttcgcc atccacaacg
     2281 agggcagcaa ggtcaccgag gtctacgtgc tgcgctccga cgagagcatc gtcgccgagc
     2341 gggagaacat cggcccgggc acctcggccg acctcaccgt ggcgctgcgc gcgggcgact
     2401 acgtcgtgcg ctgcaagccc ggcatggccg gggaggggat cgccgcaccg ctcgccgtga
     2461 ccggcggacc ggcgtcggcg gctccggcgg ccgatgcccg gctcgagcag gccgtcaccg
     2521 actaccgggc ctacgtgctc gctcaggtgg acgactcgat caagctcacc gaggcgctcg
     2581 tcgcggcggt caaggccggc aacgtcgccg aggccaagcg gctctatgcc ccctcgcgcg
     2641 tcgggtggga gagcatcgag ccggtggccg agagcttcgg cgacatcgac ccgcgcgtcg
     2701 acctgcgcga ggctgacctg gccgcgggcg aggagtggac cggctggcac cgcatcgaga
     2761 agggcctgtt cgtctccggc agcaccgacg ggcttgcgtc gtacggcgac cagctgctcg
     2821 ccgacctgcg ggagctgcgc cagcgggtgc ccgacgccga gatcacgccg acgtcgatgg
     2881 ccaacggggc caaggagctg ctcgacgagg tggcgaccgg caaggtcacc ggcgaggagg
     2941 aggcgttcag ccacaccgac ctcgtcgact tcgaggccaa cgtggtcggg gcagagaagg
     3001 tgttggtgct gctgcacgag gtggtcaccg acaacgagcc cgagctggcc gccaccctcg
     3061 acaaggagtt cggcgtcgtg ctcggcctgc tcgacaagca ccgggacggc gacgggttcg
     3121 tcagctatga caccgtcggt gacgacgacc gtcgcgtgct ctccgacgcg gtcaacgcgc
     3181 tggccgagcc gctctccaac ctggcggccg cggtcacgcc ccgatgaccg gtccggtgct
     3241 ctcgcgtcgg cggctgctcg gcgtcgcggg cgtcggtctg gtcggtgcgg ctgccggcgt
     3301 gggcggagcc tcgttggtcg agaggtccgg cgaggacacc ggcgaccggt cgagcctcgc
     3361 agcggcgggc tcggcgaccg cagtggtgtt ccacggtgcc caccaggccg gcatcgccac
     3421 cccggtgcag gaccggctgc agatcaccgc gttcgacctc accaccgact cccgcgacga
     3481 gctggtcgcc ttgctgcggg agtggaccag ggccgccgag gcgatgaccg ccggacgtga
     3541 cgtcgggcgt ggtgcggtcg acggccgcgc cgaggcaccg cccgatgaca ccggggaagc
     3601 cctcgggctg ccgccgtcgc ggctgaccct gaccgtgggc ttcggcccga gcctgttcga
     3661 gcgggacggg gtcgacaggt ttggcctgcg tgacgcgcga ccgggcgcgc tcatcgacct
     3721 gccgcgcttt cgcaacgacg ccttggagcc ggctcgctgc ggcggcgaca tcgtcgtaca
     3781 ggcctgtgcc gacgacccgc aggtggccgt ccacgcgatc cgcaacctgg cccggatcgg
     3841 gtttgggcgg gcgatcgtgc gctggtcgca gctgggcttc ggcaagacgt cgtcgactac
     3901 ctcggacgag gtgaccccgc gcaacctgtt cggcttcaag gacggcacca acaacatcac
     3961 cggtgcccag cacgaccggc tcgaccggca cgtctgggtc ggcgacgagg cggccgggcc
     4021 tgcgacctgg atgcgcggcg ggacctatct ggtggcccgg cggatcggga tgcgcatcga
     4081 gacatgggac cgggagtcgc tggccggcca ggagcagatg gtcgggcgca ccaagcggga
     4141 gggtgcgccg ctggggcaga cccatgagcg cgatgccgtc gacctgtcgg cactgccggc
     4201 cgactcgcac gtggcgcttg cacatcccga cgccaatggc gggcatgcca tcttgcgacg
     4261 gggctattcc tacgtcgacg gcagcgacgg gctcggtcgg ctggacgccg ggttgttctt
     4321 catcgcattc tgtcgcgacg cgggtgtgca gtacgtcccg atgcaggagc ggatggcccg
     4381 cctggacacc atggccgaat accttgtcca caccggctcc gggctgttcg cctgtccacc
     4441 gggcgtagac gggccggatg actactgggg ccgggcgctg ttcgaggcct gatcctttcg
     4501 tgcggttctg atggacccgc cgccgcggac gcctaccgtg gcagatgcac ggtcggtacg
     4561 acgacgggag cgctgcccat gtcgtatgta cgacgcttcg accatgtcgg cgtcacggtc
     4621 gcagatctcg atgtggtgac cgccttcttc gtggcgctcg gcctggaggt cgaaggccgg
     4681 ggattcgtcg agggtcagtt cttggacacg gtgtgcggca ttcccgactc gcgcaccgag
     4741 atcgtcatgc tgaggtcgcc cggtgaaggc gcccgcctag agctcgccag gtttgtgcgg
     4801 cccgactccg tgccgggctc accgaccgcc atggccaacg agctgggact gcgcaatgtc
     4861 gccttcgagg tcaacgacct gcaggcggcc gtcgactggg cagcgagcga gggttacgga
     4921 ctcgtcggtg gcatcggcgc gtacgaggat gcgtggcgga tggcctacgt ccgggggccg
     4981 gaaggcatca tcgtcgcgct tgccgagcgc atcggctgag cttgcacggt gaccgctctg
     5041 ccggtcgcgc aggccgtcac tcgccggtca gcgtcgggtg ggtgctcggg tgcgggtgtg
     5101 tccggtcggg gtggtccagg tcagaccttc gggtccatga cgggccacgc gccagcggcg
     5161 ggattgtttg agccggtggt gagtgcggca caggcaggcc aggttgcggg ggtgagtcgg
     5221 cccgcccggc catgcgcggg tgtggtcgat gtcgcagcga gacgcgggca cccggcagcc
     5281 ggggaatcgg cacacccgat cgcgggcgcg gaccagccgg gccatcgccg ccgggatcac
     5341 gtagggcccc gggtcctcga accactcgcc gccggggcca tcgtcagcgt cagcgccccc
     5401 gccaccgctg ggcgagtcgc caccgccggt tgagccgccg cctccgccac ctccgcggtc
     5461 gggcggccca ccaccggggc cgcccctgtc atcgtcaggg ggtcggtcgt cgtccggatc
     5521 agggtccttg ccgtcgtcgc cgggctcggg atcgtgaggc aggtcggtca gcggcagcgg
     5581 gctggtctgg tcgggaccaa gggtgagcag cgcgccggtg gtcgggtcgg tgaccgcgac
     5641 cgcggtggtg gtctcggcgt ctcggatcag cgcccgggcg tgggcggggg tgatccagcc
     5701 gtgcgaggac agccaggccg gggtcaggct cttcccggtg agcgcgtcca gcgtgatcgc
     5761 cagctccagg cgcacctgcc ggcgaaacgc cccacccgcc ccactggtcg cgtcccggct
     5821 gccggccggc acgtcgctgg ctggggcgcc cagggcaggg tgcccgcaga ccaaagcgac
     5881 cagcgcgtcg gcccggcggg cgtccatccc ccgcggatcg tcggcgccgc gggcccgggc
     5941 ccacgcgtcg caggccgacc agaccgccaa tgcgtcggca ccttcgagcc ctgcggcgtg
     6001 gatcgtgccg gtgccggagg ccgggtccgg ccacagctgc agccgccgct cggcctgcgc
     6061 cttcgtgcgc cgccggtcca acgcggctgc cctcacgtgg gccagcgcct tgctcaccgc
     6121 gacccgcagc tgccccgagg tcagctcgcc cgcctcgggc agcaccgccg cctccaccgc
     6181 ccgggcggtc gtggcgggct gaccggccaa cccgttgacc agcacccggg cccggccctg
     6241 atcgatccgc ccctcccaca acgccgaccg ggtcgccggc agctcgtcga ggtcggtcgc
     6301 gaacgagagc atcactcccg cggtgcgccg ggtggcgtgc aacgccaacg cgatctcatc
     6361 cacgcagaac tcgttgacct gctcccgcca gcgcgagtgc accccttcac cgaccgggtc
     6421 cagccgccgg tgcgccagct ccaccatccg cgcggcccgc tcggccaccg actaaagagg
     6481 cccagcgaca ctctcggtct gccacaggtc gatgaaccga tgaccgagct cgtgcagcag
     6541 gcaccgcatc agcggcaggc tgatgccgac cacgttgtgc gggtctcctt cgatgccgtc
     6601 gatgaaaggc ccacccagcc catcgatcgt gaaggccccc gcaacgtgca agggttcacc
     6661 ggtcgcgacg tacgcctcga tctcctcgtc atcgacctcg gcgaaccgga cgacggtcga
     6721 cgccaggccc gacaccgtcc gtccggaccc gaggtccacc aggtggtgac cggtgtgcag
     6781 ggtcccgcga cgcccgctca tgcgctgcca tcgatcgatc gcctcggcgg cgctggccgg
     6841 cttgccatag gcccggccgt tgatctcgag caccgagtcg cagcccacga tgatcgcggc
     6901 cgggggcagg ccgtcggtcg ctgccaccgc ctcggccttg gcccgggcca gtaaccgcgc
     6961 gatgtcagtt gccgattccg cgcccgcagc ggcggtcacg gcgtcctcgt cgacgtcgga
     7021 gacccgcacc accggctcga ctccggcggc gcgaagcgtg gcaagacggg cgggggacgc
     7081 ggaggcgagc acgagacggg gcacggaccg aatcttgggc gctccacccc gtccccgcca
     7141 aacgcgcatg atcagtgcag ggccgccaaa cgcgcatgat cagtgcaggg gggtgggtca
     7201 gatctgtacg gcggtggggc gcagggcgtc gtcggcgatg gtgtgcagtg tcgcgttcat
     7261 gatgtggcgc acgaacggac ggatgaccat ccagtagcgg ccgaacctgc gccgcgactc
     7321 ggcgtcggtc gtcgccgtac ggcagccata ggtcagcagc gagcgccact cgccgtaggg
     7381 ccgcaccgag aagctcgccg cgatcttgcc ccagcccggc tcggagaagt cggcgaactc
     7441 ctcgcgcgcc acgtcgtgcc aggtgatgtc tcgctgccag aaccggccga ccgcaccgaa
     7501 ggcgatctcc tcgtcggggc gctcgccgag cagggtccag ccgggcaggt ccagcccctc
     7561 gtcggacagc cgcagctgcc ggggaatggt cggcgcgttg cccctgacct tggccggcag
     7621 cccgcggacc cacatcgagg cgctcagcag cggggagcgc accgagagga agtcgagatc
     7681 gtgcgccgcc cgataggtgc taggcacgtc ggagtcgacc acgagggtct cgtcgacggt
     7741 cacgtcgtag tgggggagca tctcgtcgag gatgctgcgc gtcgtctcgt tcacccctcg
     7801 acggtgcggc caacccgcgg gcgcggacag ggccgttggt cccgggtgcg ggtcagcgcg
     7861 gcaggccgct gcgtcgccac gcgcccggcc ccggacgcat cggtgggcgc agccgccggg
     7921 ccgggtcggc ccaggtcgag gccggcgccg gccggtgagg cgcgccggag gcgccgcgcg
     7981 aggtcacgac ggcgagcagc gcggccagct ggtcggcgtc gggctcgcct cgcaggaccc
     8041 gcagcagcgg cgggtcgagg gccacgctca cagcgggatg ttcccgtgct tcttcggcgg
     8101 caggctggcc cgcttggtgc gcagcgagcg cagcgcgcgc acgatcgaca cccgggtctg
     8161 cgacggggcg atcaccgcgt cgacgtagcc gcgctcggcg gcgatgtagg ggttggccag
     8221 ggtgtcctcg tagtcggcga tcagctcgcg ccggcgcgcc tcggagccct cctcgccccc
     8281 cagctccttg cgatagagga tgttgaccgc gccctgcgcg cccatcacgg cgatctgcgc
     8341 ggtcggccag gccaggttga tgtcggcccc gaggtgcttg gagcccatca cgtcgtaggc
     8401 gccgccgtag gccttgcggg tgatgacggt gaccgtcggc acggtggcct cggcgtaggc
     8461 gaacagcagc ttggctccgc gccggatgat gccgttccac tcctggtcgg tgcccggcag
     8521 gaatccgggt acgtcgacga acgtgaggat cggcacgttg aacgcgtcgc acgtgcggac
     8581 gaaacgggcg gccttctcgg aggcgtcgat gtcgagcgtg ccggcgaacg ccatcggctg
     8641 gttggccacc accccgaccg gccggccctc gacccggccg aagccgacga cgatgttggg
     8701 cgcgaacagc gcctgcacct cgacgaactc gccgtcgtcg acgacgtgct cgatcaccgt
     8761 gcgcatgtcg tagggctggt tggccgagtc gggcacgaac gtgtcgagct cgaggtcgac
     8821 gtcggtgacc tcgagctcgg cctcgacgtc gtcgtaggcc ggtggctcct cgaggttgtt
     8881 gctgggcaga aacgccagca gcgccttggc aaactcgatc gcgtcgtcct cgtcggcggc
     8941 caggtagtgg gcgttgcccg acttcgtgtt gtgggtgcgg ccgccgccca gctcctcctg
     9001 gctgacgtcc tcgccggtca ccgtcttgat cacgtcgggg ccggtgatga acatgtagga
     9061 cttctcgtcg accatcacgg tgaagtcggt gatcgccggg gagtagaccg cgccgccggc
     9121 gcacggcccc atgatcagag agatctgggg tacgacgccg gaggccagca cgttgcgctg
     9181 gaagatctcg ccgtagagcc cgagggagac cacgccctcc tggatccgcg cgccgccgga
     9241 gtcgttgatc ccgaccatcg gggcgccgat cttcatggcg aggtccatga ccttgacgat
     9301 cttctcgccg aacacctcgc cgagcgagcc gccgaagacc gtgaagtcct gggcgaacac
     9361 gcacaccggg cgcccgtcga ccgtgccgta gccggttacc acgccgtcgc cgtaggggcg
     9421 gaccgcctcc atgccgaagt tggtggtgcg gtggcgtgcc agctcgtcca tctcgacgaa
     9481 cgagcccgga tcgagcagca gggcgatgcg ctcacgcgcg gtcttcttgc ccttggcgtg
     9541 ctgcttctcg atggcgcggg ccgagccggc gcggcgagcc tcgcccacgc gtcggcgcag
     9601 gtcggcaagc ttgcctgccg tcgtatggat gtcgatgtcg gccgggacct cggtgccacc
     9661 catggcatcg gccatgcgga aacctcctgg tgcgtgggcg cccgtagcct agtcggatgc
     9721 ccgggagccg ctggtccgac ctcgagcgcc cgccgctaga cgcgcaagcg ctacgtcgtg
     9781 cgctgctggc tccgtcgggg ccgtggacct cgctcgaggt cctggccacc accgggtcga
     9841 ccaacgccga cctcgtggcg cgcgcgcgtg ccggcgcgcc ggccggtgcg gtgctcgtgg
     9901 ccgaggagca gaccgccggg cagggccggc tcgaccggcg ctgggtgacg cccccgcggg
     9961 cggcgctggc ggtgtcgatg ctgctgcgcc cgccggtgcc ggcccggcgg tggggctggt
    10021 tgtcgctgct ggtgggcctg gccgccaccg acgccgtacg gcgggtcgcg gagctcgacg
    10081 cggtgctcaa gtggcccaac gacgtgctgg tcggtgagcg caagctcgcc ggcgtgctca
    10141 tcgagcgggt cgacgcgcca cccggcccgg cggcggtcgt gggcatcggg ctcaacgtca
    10201 gcctggccga ggacgagctg ccggtcccgg aggcgacctc gctgcgggtg gccggcgccg
    10261 aggtgatcga ccgcgatccg ttgctgcggg cgctgctgcg cgcggtggcc ggccgctacg
    10321 aggcctgggt cggtgccgat ggtgacgcga cggcgtgcgg gttgcgtgcg gcgtacgtcg
    10381 accgctgcat gaccttgggc cggccggtga ccgtgcacct gccggccggc cgtcgggagg
    10441 gcggggtcgc cggttcggtc gacgagacgg gggcgcttgt cgtgcgtcga gacgacggca
    10501 ccgacttgac ggtgaccgcc ggtgacgtcg tgcacgtccg cccgggcgcg ccagactgat
    10561 ggtcgcgcga cgatgaggca ggattccccc gacggtcggt ccgaggtcgc aggaggcgct
    10621 catgggcatc ccccagcggc tgctcagtga cgatgagcgg ctggtgctgg tcctgcgccc
    10681 gcactggaag gagatcatcc cggccgcgct ggtcctgctc gtgaccgcgc cggccgcgtc
    10741 gttcctggcc gccatcacgc cggagggcgg cgctcagggc tggctgcgcc tggccgtgct
    10801 ggtcctggcc gtgctcgtcc tgctgcgctg gtcgctgtgg ccgttcctca cgtggctgac
    10861 caccacctac ggggtgacca gcgaacggat catcacccgg gagggcgtca tcgcgcgcca
    10921 gggccacgac atgccgctgt cgcgggtcaa cgacgtgtcc ttctcgcaca gcgcggtcga
    10981 gcgggtgctg cgctgcggga cgctggtcgt cgagtccggc ggcgagcgtg gccagctggt
    11041 cctgcgtgac gtcccgcggg tcgagcgggt gcagcgtgag atctaccgcc tcgccgaggc
    11101 cgacgagctg cgtcgtcgtg gccgcggcga cgagggcgcc gtgctcggcg accggtaggg
    11161 gcagggccag cgcgtggacg acctcgaccc ggccgagctc gaggagatgc tcctcgaggg
    11221 cccgcggctc taccggcgtg cccaggtcgc ccgctacgcc gacagcgacc tcggtcagct
    11281 caccaagctg tggcgggcgc tgggcttccc cgacgtgccg gagggctcgg cggccttcac
    11341 cgacgccgac ctcgtggcgc tgcgtcgggt gctgcgccta gcccgtgcag gggtcttcga
    11401 cgaggacttc atcatcgggg tcacccgggc gatgggctac cacctggccc ggctggtcga
    11461 gtgggagtac gtcgcgctgg ccgagcacgt ggccacccgt gacggggccg gctcggagga
    11521 cgccgggccg ggcgggctgg ccctgctcac cgagcacctg gtcgacttcg aggcgctgct
    11581 gctctatgtc tggcgccgcc agctcgtggc caccgcgggc cgagtgctcg gcggggccga
    11641 cgaggaggtg gcccgtcgac gccggacggt gggcttcgcc gacctggtct cctacaccca
    11701 gctctcccag cggctcgagg cgcgcgagct ggctgcgctg gtcgaccggt tcgaggcgct
    11761 ctcggccgac atcatcgccg agcacggcgg ccggctggtc aagacggtcg gcgacgaggt
    11821 gctg
//
LOCUS       DATIBB010000008         4564 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_266105, whole genome shotgun sequence.
ACCESSION   DATIBB010000008 DATIBB010000000
VERSION     DATIBB010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 4564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4564
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_266105"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(213..686)
                     /locus_tag="VLC50_00235"
     CDS             complement(213..686)
                     /locus_tag="VLC50_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019873621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HSF25922.1"
                     /translation="MATKTAAPVARDSAGSTDAEQAGKGKGKRKLLLVLALVLLVGLA
                     AAAYVVLRPGSAEAGSEKPVAGAVLKLDPINVNLAEGHYLKLGLALQLTETAVETPDG
                     SQALDLAIDTFSNREMSELGSNEARTLVKAELLKRISTAYDGAVMDLYFTEFVMQ"
     gene            complement(700..1587)
                     /locus_tag="VLC50_00240"
     CDS             complement(700..1587)
                     /locus_tag="VLC50_00240"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HSF25923.1"
                     /translation="MSSTAPAARRHRRRTDDEEPDNSERWLVTYGDMLTLLMVLFIVL
                     FAISQVDQRKFEELKSGLTEGFGAPHSVLAGSPGLLSDPPLGPEPLDLRGAAAPTVAA
                     RVAAPEPTSAASDAELKAAAVKEVESLEAIRHAIEKALAAQGLRDTVRFRYDDRGLVV
                     SIVTDRVLFPADRATLAPIGQRVLDAIAPVLKALPNGVDVEGHTNTVPVKPKFFPTEW
                     ELSSARAVTVVRYLIDHSGIAAKRLTATGFADQHPLLPATDSRASTINRRVEVVIVST
                     LPAAERALIPRLAPPGDRL"
     gene            complement(1587..2360)
                     /locus_tag="VLC50_00245"
     CDS             complement(1587..2360)
                     /locus_tag="VLC50_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019873619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein"
                     /protein_id="HSF25924.1"
                     /translation="MELATLIGLGVGFGSIFIAIILEGASPMAIILIPPMLLVLGGTF
                     GAAMAGSMMRDVKKVGRWFKTVLLAKVEPADEVVDTVVKLAERARREGLLALEDASRD
                     VDDEFLRKGLELAIDGTDPEELREILEGKLDAKRAQDRLGARFFTDMGGYSPTIGIIG
                     TVIGLIHVLGNLSEPDKLGHLIAGAFVATLWGVMMANVVWLPFGKKLARISELECQRM
                     ELIIEGILAIQAGANPRVVAQRLRSLLPAPEGAAAGKAA"
     gene            complement(2360..2692)
                     /locus_tag="VLC50_00250"
     CDS             complement(2360..2692)
                     /locus_tag="VLC50_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012947171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar FlbD family protein"
                     /protein_id="HSF25925.1"
                     /translation="MLTRLNGREFALNPDLLERAEATPDTVVTLVDGTKFVVTESVGE
                     VIDRVRDYRASVLAQAQALELDASRGLPTVPLPTGQPGHSGHSGQPGEPDHAGGHSRV
                     VPLHRREP"
     gene            2910..4091
                     /locus_tag="VLC50_00255"
     CDS             2910..4091
                     /locus_tag="VLC50_00255"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HSF25926.1"
                     /translation="MGIAPVDHRVDWSREIDRAAFGPAAPTMVFQPIVDLRAGRVTGF
                     EALARFDDSPTPPDVWFSQAASYGVADVLESRAIAAALRARAGLPTGCFLTVNVSPDL
                     LPSDAVMEVLSAAGDLTGVVIELTEQTELTQLSAVNAALAFVRTRNAMVAVDDAGAGY
                     AGLQRLLEIRPDMVKLDRALVDRVDQDGAKQALARMLGDLSGHLDAWLLAEGVERIQE
                     LDTFAAMGVPLGQGWLFGHPSPTFSTALDASLVRRMRALGRDTPAQRESVARLVDADT
                     LTLPAGHSLGLLASSLGHQGVRVVVDHDGRPTAMLLDRAGHVGTAPVSLRVHESDPLV
                     DVAQRALLRPAAVRWDPVVCVDPNGRLTGTIHVQRLLLEAVGAPTGRGASPSSTREEP
                     R"
     gene            4088..4261
                     /locus_tag="VLC50_00260"
     CDS             4088..4261
                     /locus_tag="VLC50_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007077299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1059 domain-containing protein"
                     /protein_id="HSF25927.1"
                     /translation="MKSFTCGDVVPGCEAVFTAADEEGILAQVADHAPDCPGLTVDGL
                     DLVAAVRPHIHAA"
BASE COUNT          609 a         1648 c         1664 g          643 t
ORIGIN      
        1 tagagctgcg ggccggcacc gcgcgagcgg cgcccggccc gcccggtgcg cgaggcgcgt
       61 gagggcgcga cggtgcccag ggcgggcgtg ttcgcggcgg tgctcacgcc gcagtgttcg
      121 gccgacgccc gggccgactg aggaaaacgc cggactgctc cacccgggtg acccgagagg
      181 gacccacccg gtggagtgca gggcggtggg ggctactgca tgacgaactc ggtgaagtag
      241 aggtccatga cggctccgtc gtacgccgtg ctgattcgct tcagcagctc ggccttgacc
      301 agggtgcgcg cctcgttgga gcccagctcg ctcatctccc ggttgctgaa cgtgtcgatg
      361 gccaggtcga gcgcctgcga cccgtcgggg gtctccacgg ccgtctcggt cagctgcagg
      421 gccagcccga gcttgaggta gtgcccctcg gccaggttga cgttgatcgg gtcgagcttg
      481 agcaccgcgc cggccaccgg cttctccgag ccggcctcgg cactgcccgg acggagtacg
      541 acgtaggcgg ccgcggccag cccgacgagc agcaccagcg cgaggaccag caggagcttg
      601 cgcttgccct tgcccttgcc ggcctgctcg gcgtcggtgc tgccggcgct gtcgcgggcg
      661 acgggcgcgg cggtcttggt ggccatggtc actcctctct cagagccggt cgcccggcgg
      721 ggcgagcctg gggatcaggg cccgctccgc ggccggcagc gtggacacga tgacgacctc
      781 gacccgtcgg ttgatcgtgg atgcgcgcga atcggtggcc ggcagcagcg ggtgctggtc
      841 ggcgaacccg gtggcggtca accgcttggc ggcgatcccg ctgtggtcga tgaggtagcg
      901 caccacggtg accgcgcgcg cgctggagag ctcccactcg gtcgggaaga acttcggctt
      961 gaccggcacg gtgttggtgt ggccctcgac gtcgaccccg ttgggcagcg ccttgaggac
     1021 cggggcgatc gcgtcgagca cccgctggcc gatcggcgcc agggtggccc ggtcggccgg
     1081 gaagagcacc cggtcggtga cgatgctgac gaccagtccg cggtcgtcgt accggaaccg
     1141 gaccgtgtcc cgcaggccct gggcggccag cgccttctcg atcgcgtgcc ggatcgcctc
     1201 cagcgactcg acctccttga ccgcggccgc cttgagctcg gcgtcggagg cggcactggt
     1261 gggctcgggg gcggccaccc gggcggcgac ggtcggcgcg gcagctccgc gcaggtcgag
     1321 cggctccggg cccagcggcg ggtcgctgag cagcccgggg gagccggcga gcaccgagtg
     1381 gggtgccccg aagccctcgg tgaggccgct cttgagctcc tcgaacttcc gctggtccac
     1441 ctggctgatc gcgaacagca cgatgaacag caccatgagc agcgtgagca tgtcgccgta
     1501 ggtgaccagc caccgctcgg agttgtccgg ctcctcatca tcggtacgac gccggtgccg
     1561 gcgcgccgcc ggggcggtcg aggacatcag gccgccttgc cggccgcggc gccctcgggc
     1621 gcgggcagca gtgagcgcag ccgctgggcg accacacggg ggttggcccc ggcctggatg
     1681 gccaggatcc cctcgatgat cagctccatc ctctggcact cgagctcgct gatccgagcc
     1741 agcttcttgc cgaacggcag ccagacgacg ttggccatca tgacgcccca cagcgtggcg
     1801 acgaacgcgc cggcgatgag gtggcccagc ttgtcgggct cgctgaggtt gcccagcacg
     1861 tggatgaggc cgatgaccgt gccgatgatg ccgatcgtcg gcgagtaacc gcccatgtcg
     1921 gtgaagaacc gggcgcccag ccggtcctgg gcgcgcttgg cgtcgagctt gccctcgagg
     1981 atctcgcgca gctcctcggg gtcggtgccg tcgatggcca gctcgaggcc cttgcgcagg
     2041 aactcgtcgt cgacgtcgcg ggaggcgtcc tccagcgcga gcaggccttc gcggcgagcc
     2101 cgctcggcca gcttgaccac cgtgtcgacg acctcgtcgg ccggctccac cttggccagc
     2161 agcacggtct tgaaccagcg gccgaccttc ttgacgtcgc gcatcatcga cccggccatg
     2221 gccgcgccga acgtgccgcc gaggacgagc agcatcgggg ggatcaggat gatcgccatc
     2281 gggctggcgc cctcgaggat gatggcgatg aagatcgagc cgaacccgac accgagcccg
     2341 atcagagtcg cgagctccat cacggctccc ggcggtgcaa cgggacgacg cgggagtggc
     2401 cgccggcgtg gtcgggctct ccgggctggc cggaatggcc ggagtggccg ggctgaccgg
     2461 tgggcagcgg caccgtgggc aggccacggg aggcgtccag ctcgagggcc tgcgcctggg
     2521 cgagcaccga ggcccggtag tcgcgcaccc ggtcgatgac ctcgcccacg gactcggtga
     2581 cgacgaactt ggtgccgtcg accagggtga cgaccgtgtc gggggtcgcc tcagcgcgct
     2641 ccagcaggtc ggggttcagc gcgaactccc gcccgttcag gcgcgtcagc atgatcacgt
     2701 cgtccgtcct tggctccgtg tgcggggccg atccgtggcc ctcgacccca cgatcgggcc
     2761 ggggccggcc gacctgagca aatgtgtccg ccgggacctc gcgccgaccg ggtcgctccg
     2821 cccgcccgga cccctcaagg cgggtcggcg gccgccgatg gggaggggag cccccgcgcc
     2881 cgggcggagg cggtgggaag ggagacgtgg tgggcatcgc gccggtcgac catcgggtcg
     2941 actggtctcg cgagatcgac cgagcggcgt tcggcccagc ggccccgacc atggtgttcc
     3001 agccgatcgt cgacctgcgc gccggccggg tgaccggctt cgaggcgctg gcccgcttcg
     3061 acgactcgcc cactcctccc gatgtctggt tcagccaggc cgcgtcgtac ggcgtagccg
     3121 atgtgctcga gtcgcgcgcc atcgccgccg cgctccgggc ccgcgccggc ctgccgaccg
     3181 gctgcttcct caccgtcaac gtcagccccg acctgctgcc cagcgacgcg gtgatggagg
     3241 tgctcagcgc cgccggcgac ctgaccgggg tggtcatcga gctcaccgag cagaccgagc
     3301 tgacccagct gtcggcggtc aacgccgcgc tcgccttcgt gcgcacccgc aacgcgatgg
     3361 tcgccgtcga cgatgccggc gccggctacg ccgggctgca gcggctgctc gagatccgcc
     3421 ccgacatggt caagctcgac cgggccctcg tcgaccgggt cgaccaggac ggtgccaagc
     3481 aggcgttggc tcggatgctc ggcgacctgt ccgggcacct tgacgcgtgg ctgctcgccg
     3541 aaggggtcga gcggatccag gagctcgaca cattcgcggc gatgggcgtg ccgctgggcc
     3601 agggctggct gttcgggcac ccctccccca ccttcagcac cgcgctggac gcctcgctcg
     3661 tgcgacggat gcgcgcgctg ggccgtgaca cgccggcaca gcgggaatcc gtcgcccggc
     3721 tcgtcgacgc cgacacgctc accctgcccg ccggccactc gctcgggctg ctcgcctcgt
     3781 cgttggggca ccagggcgtc cgcgtcgtcg tggaccacga cggccggccc accgcgatgc
     3841 tgctcgaccg cgccgggcac gtcggcaccg cgccggtgtc gttgcgagtg cacgagtcgg
     3901 acccgctcgt cgacgtcgcc cagcgagcgc tgctgcgacc ggccgcggtc cgctgggacc
     3961 ccgtcgtgtg cgtcgacccc aacggtcggc tcaccggaac catccacgtc cagcgcctgc
     4021 tgctcgaagc ggtcggcgcc cccaccggac gtggcgcatc accgagctcc acccgagagg
     4081 aaccccgatg aagtccttca cctgcggcga cgtcgtcccc ggttgcgagg ccgtgttcac
     4141 cgcggccgac gaggagggca tcctggccca ggtggccgac cacgcgcccg actgcccggg
     4201 gctgaccgtc gacggcctcg acctggtggc cgccgtacgc ccccacatcc acgccgcctg
     4261 accgccgcgc cccgcgcgag gccccgcggc cgcccccgcg cgaggcgccc gcgcgaatga
     4321 ccacgccaag tgggcgttcg ctcgcttggc aaggcgtgca cgccttgcca agtgccacaa
     4381 cgccttgcct agtggccatc tacgaagggg ccgcctgagg gcgatcagcg ggttacgccc
     4441 ctaggcgctc cacacatcgg gcatatcgcc ctggataacg acgtgcaccg gcgtaacgac
     4501 gtgtacgggc gtaacgcccc caggccggtt ggggtgatgg gccaggatgc gcccggaggg
     4561 gcag
//
LOCUS       DATIBB010000009         2552 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279 k141_3447,
            whole genome shotgun sequence.
ACCESSION   DATIBB010000009 DATIBB010000000
VERSION     DATIBB010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2552
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3447"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            211..816
                     /locus_tag="VLC50_00265"
     CDS             211..816
                     /locus_tag="VLC50_00265"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015796276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HSF25928.1"
                     /translation="MPAPATGLTVVAANEVSCEDLQTVFGARGQPARCQCQRYRLRPR
                     ESFASFPVEERAHRLRQQTDCGHPGSGTTSGLVAYLEDEPVGWCAVEPRTAYAGLLRN
                     NRVPWVGRAEDKSDDGVWSLTCFVTRVGFRRRGVSRTLAGAAVDFARSHGAHALEGYP
                     MTTTDVILEELHVGTANVFASAGLREVSRPTLRRVVMRVDF"
     gene            complement(832..1266)
                     /locus_tag="VLC50_00270"
     CDS             complement(832..1266)
                     /locus_tag="VLC50_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010593955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HSF25929.1"
                     /translation="MDQSMSLLTLGVRDLAVARRFYVEGLGWRPTLEVPGDVTFIKVS
                     HGLLLALWSRASLAAEAGGVYQGDEPAPIALAHMVPDAAAVARVLDEAVAAGATLIAP
                     ATQREWGGVTGYFADPDDYRWEVAWNPSTELFAGEGEVVGPR"
     gene            complement(1271..1993)
                     /locus_tag="VLC50_00275"
     CDS             complement(1271..1993)
                     /locus_tag="VLC50_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018352665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC N-terminal beta barrel domain-containing
                     protein"
                     /protein_id="HSF25930.1"
                     /translation="MPVGNLMAIRRFPVKSMAGESLASAVIDTRGLVGDRLWAVIDAD
                     GKLGSGKNTRRMRRIDGLLRMQAAYDGSAALVPRVRLADGREAWADDPAVHGLLTGAV
                     GQPVTLARETAVSHYDDGPVSIVTTAALRALARVHETPVDPRRFRANLLVDWPGDDWV
                     EDGWVGRVVTVGEVRLRVDKPLQRCVMVDMAQQPVAELPDDGTVLKTLAAHHALDFAV
                     LAHVERGGAVRLGDPVTVLPEG"
     gene            2062..>2552
                     /locus_tag="VLC50_00280"
     CDS             2062..>2552
                     /locus_tag="VLC50_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006142975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HSF25931.1"
                     /translation="MQFGRTYEEFEVGATYKHWPGKTVTEYDDHLFCLLTMNHHPLHL
                     DAHYAEETTQFGRNVVVGNYVYSLLLGMSVPDVSGKAIANLEIESLRHVAPTFHGDTI
                     YGETTVLDKRESTSKDDRGIVHVETRGYNQDGTLVCVFRRKVMVPKDSYLAARGGEQP
                     GRPT"
BASE COUNT          380 a          965 c          862 g          345 t
ORIGIN      
        1 ttcctcgagc cgcgccggcg ggagccgggc gggccgcacg tcttcctcgt caccgcctcg
       61 ctgtggccgc actggccgga cgagcgggtg ctcgcctcct cgcgcgccca cggcgtgccg
      121 accacctggg agctctagcc ctccctccgc gccccgtgat gcacggggaa ggcctgacgg
      181 cgtgccggcc cgacctgaac aatcgtgccc atgccggcac cggcaaccgg gctcaccgtc
      241 gtggccgcca acgaggtgag ctgcgaagac ctgcagacgg tcttcggcgc gcgcgggcag
      301 ccggcgaggt gccagtgcca gcgctacagg ctgcggccac gcgagtcctt cgcctccttc
      361 ccggttgagg agcgcgcgca ccggctgcgg cagcagaccg actgcggtca ccccggctct
      421 ggcacgacca gcggtctcgt cgcctatctc gaggacgagc cggtcgggtg gtgcgcggtg
      481 gagccccgca ccgcctatgc cggcctgctg cgcaacaacc gggtcccctg ggtgggccgc
      541 gcggaggaca agtccgacga cggcgtctgg tcgctgacgt gcttcgtcac acgggtcggc
      601 ttccgcaggc gcggcgtcag ccgcacactc gcgggcgcgg cagtggactt cgcccgctcg
      661 cacggcgctc atgcactcga gggctatccg atgacgacga ccgacgtgat cctcgaggag
      721 ctgcacgtcg gcaccgcgaa cgtctttgcg agcgcgggct tgcgcgaggt cagccgcccg
      781 acgctgcgac gcgtcgtgat gcgggtcgac ttctgaggcg ctgaccgcga ctcagcgagg
      841 ccccacgacc tcaccctcgc cggcgaacag ctcggtactc gggttccagg ccacctccca
      901 gcgatagtca tcggggtcgg cgaagtagcc ggtgaccccg ccccactcgc gctgcgtcgc
      961 cggggcgatc agcgtcgcgc cggcggccac cgcctcgtcc agtactcggg ccaccgcggc
     1021 ggcgtcgggc accatgtgcg ccagcgcgat cggcgccggc tcgtcgccct ggtagacccc
     1081 gccggcctcg gccgccaagc tcgcgcgcga ccacagcgcg agcagcagcc cgtggctgac
     1141 cttgatgaac gtcacgtcgc cgggcacctc gagggtgggc cgccagccca gcccctcgac
     1201 gtagaaacgg cgggccaccg caaggtcgcg cacccccaag gtgagcaggc tcatcgactg
     1261 gtccaacgga tcatccttcc gggagcacgg tcactgggtc gccgaggcgc acggcgcccc
     1321 cgcgctccac atgggcgagt acggcgaagt cgagcgcgtg gtgtgcggcc agcgtcttga
     1381 gcaccgtgcc gtcgtcgggc agctccgcca ccggctgctg ggccatgtcc accatcacgc
     1441 agcgctgcaa cggcttgtcc acccgcagcc ggacctcgcc gacggtcacc acccgcccga
     1501 cccagccgtc ctcgacccag tcgtcaccag gccagtcgac gagcaggttg gcccggaacc
     1561 gtcgtggatc gaccggcgtc tcgtgcaccc gcgcgagcgc ccgcagtgcc gcggtcgtga
     1621 cgatgctcac cggcccgtcg tcatagtgcg agacggccgt ctcgcgggcc agggtcaccg
     1681 gttgcccgac cgcgccggtg agcagcccgt gcaccgcggg atcgtccgcc caggcctcgc
     1741 gcccgtcggc cagccgcacc cgcggcacca gcgccgcgct gccgtcgtac gccgcctgca
     1801 tccgcagcag cccgtcgatg cggcgcatcc gccgggtgtt cttgccgctg ccgagcttgc
     1861 cgtcggcatc gatgaccgcc cagagccggt cgccgaccag gccgcgggtg tcgatgactg
     1921 ccgacgccag ggattcaccg gccatcgact tgaccgggaa gcggcggatc gccatgagat
     1981 tgcccacagg cacgcgcgcc aacctatccg gcctgcgcca gtagggtcgc gccagagccg
     2041 cccgacgacg aaggagcacc catgcagttc ggtcggacct acgaggagtt cgaggtcggc
     2101 gcgacctaca agcactggcc gggcaagacg gtcacggagt acgacgacca cctgttctgc
     2161 ctgctgacca tgaaccacca cccgctgcac ctcgacgcgc actacgccga ggagaccacg
     2221 cagtttggcc gcaacgtcgt ggtgggcaac tacgtctatt cgctgctgct gggcatgtcg
     2281 gtgcccgacg tgtccggcaa ggcgatcgcc aacctcgaga tcgagtcgct gcgacacgtc
     2341 gcgccgacct tccacggcga caccatctat ggcgagacca ccgtgctcga caagcgcgag
     2401 tccacgtcca aggacgaccg cggcatcgtg cacgtggaga cccgtggcta caaccaggac
     2461 ggcacgctgg tgtgtgtctt ccgccgcaag gtgatggtgc ccaaggacag ctacctggcc
     2521 gcgcgcggcg gggagcagcc cgggcggccc ac
//
LOCUS       DATIBB010000010         6148 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_925381, whole genome shotgun sequence.
ACCESSION   DATIBB010000010 DATIBB010000000
VERSION     DATIBB010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 6148)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6148)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6148
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_925381"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1641)
                     /locus_tag="VLC50_00285"
     CDS             complement(<1..1641)
                     /locus_tag="VLC50_00285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF25932.1"
                     /translation="MTQRDDGHSSWASTGPRVRRPLDAAWLAVALAGTVLVIALSGLT
                     SQTLIGVGEDLSGLGSAIPGFVTGAITLGVSIVALLAAPGVFTVLLVRGHGRTAALAG
                     TAGLVAAGLAMLAGWGIRLIDDRQLGLAFTPSAGGLPDTWPFLAFLVAVLAVPRFSER
                     SRSMRLVRWSVALMVFLTLLDGRTTVSAVAVSLLVAGGAVTGTLFVAGVPGVRVDADA
                     VRDTLAAGGFPVQVVHGDDTAGERLSISDAFDSFRVDDSYDVVVLTRDRIGAGLVYRL
                     WRRLRLRGEVQPPLRFSLRRSVERELLLANATVAAGVRSPRMLAALPVRSAHTVDELH
                     AVDGPDAVALVQARVLTRGLTTFEGEVPDGVLKDVWVQLSGLRRAGIAHRHLNVRTLR
                     VDDQDRVWVVGAGAGEVAADLVALNLDVTQTLVELAMLAGVERAVSSALEGLGPEVVA
                     DAAVLLQPVLLNRETRAALREHRGLLNDLRERIGATVEVPLASPRVEKFRPRTVITGI
                     AAVFALYLVVSSLAGVDVAGVLRQANVGWMVAGVVAIAV"
     gene            complement(1718..2299)
                     /locus_tag="VLC50_00290"
     CDS             complement(1718..2299)
                     /locus_tag="VLC50_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WHG domain-containing protein"
                     /protein_id="HSF25933.1"
                     /translation="MPRAGLTPSRVVAEGADLADEVGLDRLTLAAVASRLGVRLPSLY
                     KHVAGLDALRQGLAVQALHELAQALSAATVGRSRYDALAPMAAAYRDYARAHPGRYAA
                     TLRAPDPTADAHAEAARAVLTIVLSVLAGYGLTGDRAIDATRALRSALHGFVSLEAGG
                     GFALDRDAAESFTAMVADLDRAFSATPELAGEH"
     gene            complement(2292..3113)
                     /locus_tag="VLC50_00295"
     CDS             complement(2292..3113)
                     /locus_tag="VLC50_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012725756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HSF25934.1"
                     /translation="MAPSTDHTRYLDRGEGRLAYDVTGEGPLVVLVPGMGDVRSVYRF
                     LAPALVDAGYRVASLDLRGHGESDTTFTSYDDRALGSDILALIEHLGGPATVAGNSMG
                     AGAAVWAAAQRPEAIGALALLGPFVRDVPTNAAKMVMFRLMLAKPWGKAAWLSWHRRL
                     YPTRPPADLGAQQALIRASLERPGGWDAFAATTRTTHAPAEASLDSVHVPTLVVMGER
                     DADFASAQAEAELVARRLRGQVVMVAGAGHYPQAEMPELVIPAVLGFLAQSAARA"
     gene            complement(3182..3826)
                     /locus_tag="VLC50_00300"
     CDS             complement(3182..3826)
                     /locus_tag="VLC50_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003802583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HSF25935.1"
                     /translation="MSQSPGDFPANPRFARAYLRVAPKAEDRGVREHRRELLAGLTGV
                     VCEVGAGPGLTFAHYPPAVTQVIAIEPEPTLRAHAERAAASAPVPVEVRAGVAEALPV
                     ENASCDAVVLALVLCTVPDPPRALTEVRRVLRPGGTVRVYEHVRSTHGLVAAAEDLIT
                     PLWARLAGGCHPNRDPVASLADAGFAVVTVRRFGFGPTRGLPPVAHVIGRATRP"
     gene            complement(3893..3968)
                     /locus_tag="VLC50_00305"
     tRNA            complement(3893..3968)
                     /locus_tag="VLC50_00305"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3933..3935),aa:His,seq:gtg)
     gene            complement(4043..4678)
                     /gene="orn"
                     /locus_tag="VLC50_00310"
     CDS             complement(4043..4678)
                     /gene="orn"
                     /locus_tag="VLC50_00310"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019878088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HSF25936.1"
                     /translation="MNGRDERIVWIDCEMTGLDLGRDALIEVAALVTDSELTILGDGV
                     DVVIKPPAEALATMPDVVKEMHTASGLLDELPDGLTMAEAEARVLDYVREWAPEPRRT
                     PLGGNSVGTDRTFLARDMPALEGHLHYRNVDVSSIKELARRWYPRVYFSSPAKHGNHR
                     ALGDIRDSIAELRYYRATVFVPAPGPDSATARAAAAALAGPGAEAEPPAAG"
     gene            4719..5879
                     /locus_tag="VLC50_00315"
     CDS             4719..5879
                     /locus_tag="VLC50_00315"
                     /inference="COORDINATES: protein motif:HMM:NF024760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HSF25937.1"
                     /translation="MVTRGLRPMRGSGVWLGLLTVFVLVCMGAAAVLLVGWLTEASGV
                     VGFLAGLSLALVPVAPVLAALMWLDRYEPEPAGLLAFCFAWGATVAAAVAVVFNTVAA
                     QALTAAGTDGLTTTATVIAPVVEEAAKGILLVALLWWRRDEIDGIVDGIVLGATIALG
                     FAFTENVLYFGQAFLLGREGETGQQFFALGLTFVLRGVLGPFAHPLFTACTGLGIGIA
                     AGSRSRSVRVVAPALGYLAAVVLHAMWNVAAIAGLAGFFSAYLLFMVPVFTVAVLVAV
                     LARRRERALIARHLPVYVEAGWLAPGEVATLACLPARRAARALAAQLGGSGAAAAVRD
                     YQRVATELAFLRARAERRGSPAGLRAHEVALLQQLAAARARLPAAPRYPLAG"
     gene            complement(5861..>6148)
                     /locus_tag="VLC50_00320"
     CDS             complement(5861..>6148)
                     /locus_tag="VLC50_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019328202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HSF25938.1"
                     /translation="GTLEDVLTRAGYEPHENDEGEIVLANCPFHSLARQHTALVCGMN
                     LHLLRGVLDELGAGGQAVLDPAPDRCCVRIYDLERDAAASPARDAAQPAKG"
BASE COUNT          785 a         2210 c         2356 g          797 t
ORIGIN      
        1 caccgcgatg gccacgacgc cagcgaccat ccagccgacg ttcgcctgcc gcagcacgcc
       61 ggccacgtcc acaccggcca gtgagctgac cacgaggtag agcgcgaaca ccgcggcgat
      121 gccggtgatc acggtgcgcg gacggaactt ctccacccgt ggcgaggcca acggcacctc
      181 gacggtggcg ccgatgcgct cgcgcaggtc attgagcagg ccgcggtgct cgcgcagtgc
      241 ggcacgggtc tcccggttga gcagcaccgg ctgcagcagc acggcggcgt ccgcgacgac
      301 ctcgggaccg agaccctcca gcgccgagga gacggcgcgt tcgacgccgg ccagcatcgc
      361 cagctcgacg agggtctgcg tgacgtcgag gttgagcgcc accaggtcgg cggccacctc
      421 gccggccccc gcccccacga cccagacccg gtcctggtcg tcgacgcgca gcgtgcgcac
      481 gttgaggtgc cggtgagcga tgccggcccg gcgcagcccg ctcagctgga cccagacgtc
      541 cttgaggacc ccgtcgggga cctctccttc aaacgtggtg agcccgcggg tcagcacccg
      601 ggcctgcacc agggcgacgg cgtcgggtcc gtcgacggcg tgcagctcgt cgacggtgtg
      661 ggccgagcgg accggcagcg cggccagcat ccgcggtgag cgcaccccgg cggctacggt
      721 cgcgttggcc agcagcagct cacgctcgac ggagcggcgc aggctgaacc gcagcggcgg
      781 ctggacctcg ccgcgcagcc gcagccggcg ccagagccgg tagaccaggc cggcgccgat
      841 gcggtcacgg gtgaggacga ccacgtcata gctgtcgtcg acgcggaagc tgtcgaaggc
      901 gtcggagatc gacagccgtt cgcccgcggt gtcgtcaccg tgcacgacct gcacggggaa
      961 gccgccggcg gccagggtgt cgcggaccgc gtcggcgtcg acccgcacgc ccgggacccc
     1021 tgccacgaac agggtgccgg tgaccgcgcc gccggccacc agcaggctca ccgccaccgc
     1081 cgagacggtg gtgcgcccat cgagcagggt gaggaagacc atcagcgcca ccgaccagcg
     1141 caccagccgc atcgaccggg agcgctcgga gaatcggggt acggcgagca cggcgacgag
     1201 gaaggcgagg aagggccagg tgtcgggcag cccgccggcc gacggggtga acgccaggcc
     1261 gagctgccgg tcgtcgatca gccggatccc ccagccggcg agcatggcca gcccggcggc
     1321 gaccagcccc gcggtcccgg ccagcgcggc ggtccggccg tgcccgcgga ccagcagcac
     1381 ggtgaagacc ccgggggcgg cgagcaacgc gacgatcgag acgcccagcg tgatggcgcc
     1441 ggtgacgaag ccggggatgg cgctgcccag cccgctgagg tcttcaccga ctccgatgag
     1501 ggtctgcgac gtgagcccgg acaaggcgat caccagcacg gtccccgcca gggcgacggc
     1561 cagccacgcg gcgtccagcg gacgtcggac ccgtggaccg gtgctggccc acgaggagtg
     1621 gccgtcgtcg cgctgtgtca tcgtggcaat cctggcatgc gggtctcgtg gccgggccga
     1681 tcacgcaccg ctctcgcgac ggcgtacatg cgcgcgatca gtgctcgccg gccagctcgg
     1741 gcgtcgcgct gaacgcccgg tccaggtcgg ccaccatcgc ggtgaagctc tcggcggcgt
     1801 cgcggtcgag agcgaacccg ccgccggcct ccaggctgac gaagccgtgc agcgccgagc
     1861 gcagcgcccg cgtggcgtcg atggcccggt caccggtcag gccgtagccg gccagcaccg
     1921 agagcacgat ggtgagcacc gcgcgggccg cctcggcgtg ggcgtcggcc gtggggtccg
     1981 gggcccgcag cgtggcggcg tagcgcccgg ggtgggctcg cgcgtagtcg cggtaggccg
     2041 cggccatcgg cgcgagcgcg tcgtagcggc tgcggcccac ggtggccgcg ctcagcgctt
     2101 gggccagctc gtgcagcgcc tgcacggcca ggccctggcg cagggcgtcc aggccggcca
     2161 cgtgcttgta gaggctgggc aggcggacgc cgagccggct ggccaccgcg gcgagcgtga
     2221 gccggtcgag accgacctcg tcggcgaggt ccgcgccctc ggcgaccacc cggctcgggg
     2281 tcagcccggc cctaggcacg ggccgccgac tgggccagga agcccagcac cgccgggatg
     2341 accagctcgg gcatctccgc ctgcgggtag tgccccgccc cggccaccat gacgacctgg
     2401 ccacgcagcc ggcgggcgac cagctcggcc tcggcctgcg cgctggcgaa gtcggcgtcg
     2461 cgctcgccca tgaccaccaa cgtcggcacg tgcaccgagt cgaggctggc ctcggccggt
     2521 gcgtgggtcg tgcgggtggt cgctgcgaac gcgtcccagc cgcccgggcg ctccagcgac
     2581 gcccggatca gggcctgctg cgcgccgagg tcggccggcg ggcgggtcgg gtagagccgc
     2641 cggtgccagc tcagccaggc cgccttgccc cacggcttgg ccagcatgag tcggaacatg
     2701 accatcttgg cggcgttggt cggcacgtcg cgcacgaagg gcccgagcag ggcgagcgcg
     2761 ccgatcgcct ccggtcgctg ggcggcggcc cacaccgcgg cgccggcgcc catcgagttg
     2821 ccagccacgg tggccggccc gccaaggtgc tcgatgagcg cgaggatgtc cgagcccagc
     2881 gcccggtcgt cgtacgacgt gaacgtggtg tcgctctcgc cgtgccctcg caggtcgagg
     2941 ctggccaccc gatagcccgc gtcgaccagc gccggggcga ggaaccggta gaccgagcgg
     3001 acgtcgccca tgcctggcac gaggacgacg aggggtccct cgccggtcac gtcgtaggcc
     3061 aggcggccct cgccgcggtc gaggtagcgg gtgtggtcag tgctcggtgc catggtggct
     3121 ctcccgttcg ctgatgtcat tagggagaag gctaagtcta ttagccaatc ttgtcaaccg
     3181 gtcagggcct ggtggcccga ccgatgacgt gggcgaccgg tggcaggcca cgggtcgggc
     3241 cgaacccgaa gcggcgcacc gtgacgaccg cgaacccggc gtcggccagg ctcgccaccg
     3301 ggtcgcggtt ggggtggcag cctccggcca gccgggccca cagcggtgtg atgaggtcct
     3361 ccgccgcggc gaccagtccg tgggtcgagc ggacgtgctc atagacgcgc accgtcccgc
     3421 ccgggcgcag cacccgccgc acctcggtca aggcccgggg cgggtcgggc acggtgcaca
     3481 gcaccagggc gagtacgacg gcgtcacagc tggcgttctc gaccggcagc gcctcggcga
     3541 ccccggcccg cacctcgacc ggcaccggcg cactggccgc ggcccgctcc gcgtgcgcgc
     3601 gcagggtggg ctcgggctcg atggcgatca cctgggtgac tgccggaggg tagtgcgcga
     3661 acgtcaggcc aggacccgcg ccgacctcgc agaccacgcc ggtgagtccg gccaacagct
     3721 cccgacggtg ctcgcgcacg ccgcggtcct cggccttggg cgcgacccgc aggtaggcgc
     3781 gcgcgaagcg cggattggcc gggaagtcac ccggcgactg cgacatcggc cacctccacg
     3841 cacgacacct gagcctcatc gtgcgcccga ggaggtgccg tcgcgggatc tttggggtga
     3901 gcgacgggac ttgaacccgc gacccccggg accacaaccc ggtgctctac cagctgagct
     3961 acgcccacca tcgccgtacg tcgccgtccg gcccggcgag tctagcggag cgccgagcca
     4021 gcccgaccgg ccaggcaggg cctcagccag ctgctggcgg ctcggcctcg gcgcccggtc
     4081 cggcgagggc tgcggcggct gcccgggcgg tcgccgagtc ggggcccggg gccggcacga
     4141 acaccgtcgc gcggtagtac cgcagctccg cgatgctgtc gcggatgtcg ccgagcgcgc
     4201 ggtggttgcc gtgcttggcg ggggagctga agtagacgcg cggataccag cgccgggcca
     4261 gctccttgat cgaggacacg tcgacgttgc ggtagtgcag gtggccctcg agggcgggca
     4321 tgtcgcgggc gaggaacgtg cggtcggtgc cgaccgagtt gccaccgagc ggggtccgcc
     4381 gtggctcggg cgcccactcg cgcacgtagt cgagcacccg ggcctcggcc tcggccatcg
     4441 tcaggccgtc gggcagctcg tcgagcaggc cggacgcggt gtgcatctcc ttgaccacgt
     4501 cgggcatggt ggccagcgcc tcggcgggag gcttgatcac gacgtcgacc ccgtcgccga
     4561 ggatcgtgag ctcggagtcg gtgaccaggg cggcgacctc gatcagggcg tcgcgcccca
     4621 ggtcgagccc ggtcatctcg cagtcgatcc acacgattcg ctcgtcgcgg ccattcatgc
     4681 gctcaaccct agggggtgct gctcataggg tcgggcccgt ggtgacgcgc ggactgcggc
     4741 cgatgcgcgg ctcgggggtg tggctcgggc tgctgacggt gttcgtcctc gtgtgcatgg
     4801 gggcggccgc ggtgctgctg gtcggctggc tgaccgaggc ctccggggtc gtcggtttcc
     4861 tcgccggcct ctcgctcgcg ctggtccccg tcgcccccgt gctggccgcg ctcatgtggc
     4921 tcgaccgcta cgagcccgag ccggccgggc tgctggcctt ctgcttcgcc tggggtgcca
     4981 cggtcgccgc ggccgtcgcg gtggtgttca acactgtggc ggcccaggcg ctcaccgccg
     5041 ccggcaccga cgggctgacg accaccgcca cggtgatcgc cccggtggtc gaggaggcgg
     5101 ccaagggcat cctgctcgtc gcgctgctgt ggtggcgccg cgacgagatc gacgggatcg
     5161 tcgacgggat cgtgctcggg gccaccatcg cgctcggctt tgcgttcacc gagaacgtcc
     5221 tctacttcgg gcaggccttc ctgctcgggc gcgagggcga gaccggccag cagttcttcg
     5281 ccctggggct gacgttcgtg ctgcgcgggg tgctcggccc gtttgcccac ccgctgttca
     5341 ccgcgtgcac cggtctgggc atcgggatcg ccgccggcag ccgcagccgg tcggtgcggg
     5401 tggtggctcc ggcgctgggc tacctggccg cggtggtgct gcacgcgatg tggaacgtgg
     5461 ccgcgatcgc gggcctggcc ggcttcttct cggcgtacct gctgttcatg gtcccggtgt
     5521 tcacggtggc ggtcctggtc gcggtgctgg cccgccggcg ggagcgggcg ctcatcgcac
     5581 gccacctgcc ggtgtacgtc gaggccggct ggctcgcgcc gggcgaggtg gccacgctgg
     5641 cctgcctgcc ggctcggcgg gcggcccgag cgctggcggc gcagctgggt gggtcagggg
     5701 cggcggctgc cgtgcgggac taccagcggg tggccaccga gcttgcgttc ctgcgggccc
     5761 gcgccgagcg gcgcgggtca ccggcggggt tgcgggccca tgaggtggcg ctgctgcagc
     5821 agctggcggc ggcccgggcc cggttgccgg cggctccgcg ctatcccttg gccggctgag
     5881 ccgcgtcgcg ggcgggcgag gcggcggcgt cgcgctccag gtcatagatg cgcacgcagc
     5941 accggtcggg ggccgggtcg agcacggcct ggccacccgc gccgagctcg tcgagcacgc
     6001 cgcgcagcag gtggaggttc atcccgcaga ccagggcggt gtgctgacgg gcgaggctgt
     6061 ggaacgggca gttggccagg acgatctcgc cctcgtcgtt ctcgtgcggc tcgtagccgg
     6121 cgcgggtcag cacgtcctcg agcgtgcc
//
LOCUS       DATIBB010000011        18767 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_1583702, whole genome shotgun sequence.
ACCESSION   DATIBB010000011 DATIBB010000000
VERSION     DATIBB010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 18767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18767
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1583702"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1966
                     /gene="tkt"
                     /locus_tag="VLC50_00325"
     CDS             <1..1966
                     /gene="tkt"
                     /locus_tag="VLC50_00325"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017593696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HSF25939.1"
                     /translation="LLFQKVMRHDPTDPDWVARDRFVLSAGHSSLTLYIQLFYAGLIE
                     LDDLTRLRTWGSRTPGHPEHGHTPGVETTTGPLGQGIGNAVGMAMAARRERGLLDPDA
                     PAGQSVFDHTIWCVASDGDIEEGVSSEASSLAGTQRLGNLVVIYDCNKISIEDDTDIA
                     LSEDTAARYRAYGWHVQEVDWTHDGHHYDEDVAALHAALLAAQAERDRPSFIVLRTII
                     AWPAPNAQNTGKAHGAALGADEVAATKAVLGLDPGASFAVADDVLGHVRGVIERGRAA
                     HAAWAPRFEDWRRSAPDRAELYDRMRVRRLPDGWTDALPAFEAGADMATRKASGTVLS
                     AIAAHLPELWGGSADLAESNNTTPNGELSFLPPDRATKAFPGDWYGRVLHFGIREHAM
                     GSILNGIALHGGTRPYGGTFLVFSDYMRPAVRLAALMGLPVTYVWTHDSIGLGEDGPT
                     HQPVEHLAALRAIPGLDVVRPADANETVVAWRTVLEHTDRPAGLCLTRQNVPTFDRSP
                     AGGFASAEGVARGGYVLLDAPDGRVDVVLVGTGSEVALAVAAREALAGEGVGARVVSM
                     PCREWFDAQPADYRDSVLPPAVRARVSVEAGVAAGWREVVGDAGRIVSLEHFGASAEY
                     RVLYREFGITADAVVAAAHESLTATGAGTG"
     gene            1993..3117
                     /gene="tal"
                     /locus_tag="VLC50_00330"
     CDS             1993..3117
                     /gene="tal"
                     /locus_tag="VLC50_00330"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HSF25940.1"
                     /translation="MSDTLAALHAQGVSIWLDDLSRERLRSGTLAALVRDRHVVGVTT
                     NPTIFQKALAEGDAYADQLADLAARGVDVGEAMRALTTHDVRWAADVLRPVYDATGGL
                     DGRVSIEVDPRLAHNPTSTIAEARALWWLVDRPNAFIKIPATEAGLPAISQALSEGIS
                     INVTLIFGLDRYSAVMEAFLDGMERARAAGRDLSTIRSVASFFVSRVDTEVDKRLDKL
                     GDPAAAALRGQAAIANARLAYQRYEQVFTSARWQALQAAGAHPQRPLWASTGVKDPAY
                     DDTRYVVDLVTDGVVNTMPEPTLDAVADHGRLRGDTVRGTYAEAAQVLEQLEGLGISY
                     ADVVGVLESEGVEKFEASWSQLAEALATSLQQTRSTQGHR"
     gene            3114..4736
                     /locus_tag="VLC50_00335"
     CDS             3114..4736
                     /locus_tag="VLC50_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HSF25941.1"
                     /translation="MSSAVAVRTAGAAADAVSRHLPTLVGDRVASRLAEQDPTLWGPA
                     VAADAAVRLSWVDLARSSRPLLTEVAALRDRLRGLGLDHVVLCGMGGSSLAPEVISRT
                     AGVELTVLDSTDPDQVRAALADRLDRTVVVVSSKSGTTAETDAGRRAYEQAFAAAGLD
                     PRERIVVVTDPGSALEELALAAGYVVVRADPHVGGRYSALSAFGLVPSGLAGADLARL
                     LDEAEAARPALVRDDPANPALLLGAALGGTAPLRHFVVLVEDPASGQRLAGFADWAEQ
                     LLAESTGKAGTGLLPVVVEDGHAPDAVGPCPDALVVELGPAESGQQPFGEPASGLRVT
                     GPLGAQLLLWELATAVAARLLRINPFDQPDVESAKVAARGLLDDRPETPAPAFVEAGI
                     EVRASAGLLDGVHDLPGAVDALLGRLGPSGYLAVMAYLDRHSDAALTDVRAALARRTR
                     RPVTFGWGPRFLHSTGQFHKGGLATGVFLQITGAPAADLPVPDRPFTFGELIAAQAQG
                     DAQVLAARGRPVLRLHLTDRAAGIARLCEVLA"
     gene            4733..6280
                     /gene="zwf"
                     /locus_tag="VLC50_00340"
     CDS             4733..6280
                     /gene="zwf"
                     /locus_tag="VLC50_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013117165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HSF25942.1"
                     /translation="MSPRTGAGRPRRNPLRDPRDKRLPRIAGPCSLVIFGVTGDLARR
                     KLMPAVYDLANRGLLPPGFALVGFARRDWADEDFGKVVYDAVREHARTPFRESVWAQL
                     AEGIRFVPGEFADDQAFDRLAAVVADLDRVRGTGGNHAFYLSIPPKLFPVVCDQLARS
                     GLSAQQRDSWRRVVVEKPFGHDLASAQALNRVVEQVFPHDAVFRIDHYLGKETVQNLL
                     ALRFANQLFEPIWNANNVDHVQITMAEDIGIGGRAGYYDDIGAARDVIQNHLLQLLAL
                     TAMEEPVSFDAHDLRAEKEKVLSAVRLPRDLRRSTARGQYAAGWQGGQYVGGYLDEEG
                     IPASSRTETYAAITLEINTRRWAGVPFYLRTGKRLGRRVTEIAVVFKRAPHLPFTHTA
                     TEELGQNALVIRVQPDEGVTMRFGSKVPGTAMEVRDVTMDFGYGHSFTESSPEAYERL
                     LLDVLLGDPPLFPHHREVELSWEILDPIEDYWASRGRPEPYPAGTWGPASADAMLARD
                     GRMWRRP"
     gene            6277..7230
                     /locus_tag="VLC50_00345"
     CDS             6277..7230
                     /locus_tag="VLC50_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020421294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase assembly
                     protein OpcA"
                     /protein_id="HSF25943.1"
                     /translation="MIIDLPSTTTAAVNKAMVELRESGGAVALGRVLTLVIVTADGEA
                     EDAIRAANDASREHPCRVIVLVKGTRRGGPRLDAQVRVGGDAGASEVIVLRVHGPLVD
                     HGASVVIPLLLPDAPVVAWWPGEPPPNPVADPIGAMAHRRITDVAACHDPVRALEDRR
                     ASYVPGDTDLAWSRTTLWRGLLAAALDQPPFERVSQATVAGGSDSPSTDLLAAWLTAA
                     LRVPVTRERTPVGSGLVSVTLTRPGAPIDLVRPDGHVATLSVAGAPDRRIALPRRSTS
                     ESLAEELRRLDPDEIYALALTKGLPRLARRAARAAASGAPA"
     gene            7227..8036
                     /gene="pgl"
                     /locus_tag="VLC50_00350"
     CDS             7227..8036
                     /gene="pgl"
                     /locus_tag="VLC50_00350"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010472988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HSF25944.1"
                     /translation="MTPGADPTATGRVVRVHPDAGLLAAAAAARLVTAMIDLQQARGG
                     VSVALTGGGLGIATLRAAADCPARDAVDWSAVDLWWGDERYLPAGHPDRNETQARAAL
                     LDRVALDPARVHPMPASDGPEGEDVDTAAARHAVELAEFTAKRAQQGLVPTLDIVLLG
                     IGSDGHVASLFPAHPARGDRRAVVGVRSSPKPPPVRVSLTMPTLCAATQVWLLAAGAA
                     KATAVAQSLVDGAPEPLPAAEVHGRERTLWLLDRAAAGSSDLAQQERPAAQ"
     gene            complement(8030..8380)
                     /locus_tag="VLC50_00355"
     CDS             complement(8030..8380)
                     /locus_tag="VLC50_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012892502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein RbpA"
                     /protein_id="HSF25945.1"
                     /translation="MASGNAIRGSRVGAGPMGEAERGESAPRFRISYWCANGHETRPS
                     FAEEAGVQAPEEWDCPRCGFPAGRDKDAPPAPPRNEPYKTHLAYVKERRSDVDGQAIL
                     AEALDKLRTTGRPY"
     gene            complement(8458..8697)
                     /gene="secG"
                     /locus_tag="VLC50_00360"
     CDS             complement(8458..8697)
                     /gene="secG"
                     /locus_tag="VLC50_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013018228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HSF25946.1"
                     /translation="MTIFEYVLSAILIITSIAMIALVLLHKGKGGGLSDMFGGGASSG
                     LGGSAVVEKNLDRLTIFTGIIWTASIVAIGLSFKQ"
     gene            complement(8804..9613)
                     /gene="tpiA"
                     /locus_tag="VLC50_00365"
     CDS             complement(8804..9613)
                     /gene="tpiA"
                     /locus_tag="VLC50_00365"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HSF25947.1"
                     /translation="MAESSHTGRVPLMAGNWKMNLNHFEAMALVQKLGWALKDKKHDF
                     TAVEVAVLTPFTDLRSIQTLVDGDRLDIRYGAQDVSAHDSGAYTGEVSGAMLAKLGCT
                     YVVVGHSERRAYHGEDDHLVNAKVKAAFRHGLSPILCVGEGLEVRREGRQVEHTLAQL
                     DGALDGVGAEAARDLVVAYEPVWAIGTGEVATPDDAQEVCAALRTRLAERYSGALADA
                     VRVLYGGSVKASNVAGIMARPDVDGALVGGASLDPDEFTSICRYRSHQLTG"
     gene            complement(9617..10819)
                     /locus_tag="VLC50_00370"
     CDS             complement(9617..10819)
                     /locus_tag="VLC50_00370"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020553575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HSF25948.1"
                     /translation="MKTIDDLGDLRGQRVLVRSDLNVPLDTAAGAPVVADDGRIRASV
                     PTIRTLLDRGARVVVVAHLGRPKGQPDPRYSLRPVADRLGELLGVEVAFATDTVGPSA
                     RAAVDALHDGAVALLENVRFNPGETSKDDAERAAFADQLAALADVFVSDGFGAVHRRH
                     ASVYDVAARLPHAAGGLVGAEVEVLQRLTSAPERPYTVVLGGAKVSDKLAVIEALLSR
                     ADQILVGGGMVFTFLAARGHRVGRSLLEADQVDTVRGYLETAERRGVRILLPVDIVVA
                     AAVEPGAEHRVVASDAIPADMVGVDIGPESAGQFATAVRESRTVFWNGPMGVFEIPEF
                     AAGTRAVAEALAATKGYTVVGGGDSAAAVRRLGFSDDAFDHISTGGGASLEYLEGKTL
                     PGLQVLEG"
     gene            complement(10846..11850)
                     /gene="gap"
                     /locus_tag="VLC50_00375"
     CDS             complement(10846..11850)
                     /gene="gap"
                     /locus_tag="VLC50_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013287466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HSF25949.1"
                     /translation="MSIRVGINGFGRIGRNFYRAVLASGADIEVVGVNDLTDNKTLAH
                     LLTYDTTLGRLGLPVSYDESNIRVGDHVVRALEERDPTALPWKELGVDIVLESTGRFT
                     DATMARAHVEAGATKVIISAPAKHEDFTVVMGVNDADYDPAAHTIISNASCTTNCLAP
                     MAKVIHDTFGIERGLMTTIHAYTADQNLQDGPHKDLRRARAAAVNIVPTGTGAAKAIG
                     LVLPQLNGKLDGFAMRVPVPTGSVTDLTVDVSRETSVAEVNAAVKAAAEGAYKRYIHY
                     TEDPIVSSDIVNDPASCIFDAGLTKVIGAQVKVVGWYDNEWGYSNRLVDLVTLVGSSL
                     "
     gene            complement(12017..13000)
                     /gene="whiA"
                     /locus_tag="VLC50_00380"
     CDS             complement(12017..13000)
                     /gene="whiA"
                     /locus_tag="VLC50_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014627522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HSF25950.1"
                     /translation="MAMTASVKDELSRLTITRLCCRKAEVSSMLRFAGGLHLVGGRIV
                     VEAELDTGVAARRLRRNIAEVFGHPSEFTVVAAGGLRRGSRYIVRVVRDGEALARQTG
                     LVDGRGRPVRGLPPQVVSGATCDAESAWRGAFLVHGSLTEPGRSSALEVTCPGPEAAL
                     ALVGAARRLSIGAKAREVRGVDRVVVRDGDAIGEMLTRLGAHDSVLAWEERRMRREVR
                     ATANRLANFDDANLRRSARAAVTAAARVKRALEILGDEAPDHLRSAGVLRLEHGQASL
                     EELGQLSEPPMTKDAVAGRIRRLLAMADRRAAELGVPGTEANLTPELLGEA"
     gene            complement(13004..14044)
                     /gene="yvcK"
                     /locus_tag="VLC50_00385"
     CDS             complement(13004..14044)
                     /gene="yvcK"
                     /locus_tag="VLC50_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020553581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HSF25951.1"
                     /translation="MPASFPSRPLADPRGGPAVVALGGGHGLAASLTALRRVTDRLTA
                     VVTVADDGGSSGRLRRELGVLPPGDLRMALAALCGDDDWGRTWSDLVQHRFRGDGELR
                     GHAVGNLLIVALWELLGDTVPALDLVGRLLRAEGRVLPMCSVPLDLEATVVGADAADP
                     DGVTSVRGQVAVASTPGRVAGLRLVPDQPPACAEAVTAVRYADWVVLGPGSWFSSVIP
                     HLLVPELAAALCETTARRAVVLNLAPQPGETSGFSPETHLEVLAAHAPGLRLDVVIVD
                     PRTVAELGPLQRVAAELGARLVVRPVSAADGSPRHDPTRLAAAFAATFAGTASARPTG
                     ERSDTTAHHGRI"
     gene            complement(14071..14964)
                     /gene="rapZ"
                     /locus_tag="VLC50_00390"
     CDS             complement(14071..14964)
                     /gene="rapZ"
                     /locus_tag="VLC50_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018382722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HSF25952.1"
                     /translation="MNISDVADGRVHAPQLIIVTGMSGAGRTTAANVLEDLGWYVVDN
                     LPPELLPLLADLGGRTQGAVARIAAVVDVRSRSFFAALREAIDELARTGSDPRVLFLE
                     ANDDALVRRFEAVRRPHPLQGDGRVVDGIVRERDMLRDLRGDADIVVDTSDLNVHELA
                     RKVAAAFPEQPTPDLRATVMSFGFKYGLPVDADLVADMRFLPNPHWVPELRQRTGLDP
                     SVRDYVLTQVGAKDFLARYHELLLMLIEGYRREGKRFVTIAVGCTGGKHRSVVMAEQL
                     AALLIADDVDAVTVHRDLGRE"
     gene            complement(14961..16964)
                     /gene="uvrC"
                     /locus_tag="VLC50_00395"
     CDS             complement(14961..16964)
                     /gene="uvrC"
                     /locus_tag="VLC50_00395"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019877458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HSF25953.1"
                     /translation="MADPASYRPATRSIPDEPGVYRFRDADRRVIYVGKAKSLRQRLS
                     SYFADLSGLHPRTQTMVTSAASVEWTVVGTEVEALALEYSWIKEFDPRFNVRYRDDKS
                     YPYLAVTLSEEFPRLQVMRGAKRKGTRYFGPYAHAWAIRETLDLLLRVFPARTCSAGV
                     FKRANRAGRPCLLGYIGKCSAPCVGRVSAEEHRAIVEDFCDFMAGQTSTYVRRLEKEM
                     QVAAANLDYERAARLRDDVKALERAMEKNAVVLGDGTDADIVAVAEDPLEAAVQVFYV
                     RGGRIRGQRGWVVEKVEDLTTADLIEHFLQQLYGANGDGWADSVPREVLVPELPPDVA
                     AVEEWLRGLRGSRVVVRVPQRGDKRALLETVARNATQALGLHKVRRAGDLTTRSQALQ
                     EIQDALELTQAPLRIECFDVSNLGADQVVASMVVFEDGLARKSEYRRFTIKTVAGQDD
                     VASMHEVITRRFRRYLEHRDRPAPLDLLEDPEAGQSRRFAYPPNLVVVDGGPPQVAAG
                     QRALNDLGIDDVVLVGLAKRLEEVWRPGQAHPVVLPRSSEGLYLLQRVRDEAHRFAIT
                     FHRQRRRKAMLESTLDGIPGLGQTRRKALMTRFGSLKRLRAATAEEIAAVPGIGPRTA
                     AAVVIALAADHPSPAVDVGTGEILDATTDPTPTTAPRDRGDPQ"
     gene            complement(17031..17444)
                     /locus_tag="VLC50_00400"
     CDS             complement(17031..17444)
                     /locus_tag="VLC50_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018557244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HSF25954.1"
                     /translation="MEQGSTAHPITRRTLLRGTAAMGVAAAGLAGCAGGGPSAAPGTE
                     LTAIADVPVGEAKVIGGSQPVVIAQPTQGQFIGHSAICTHGGCTVGTDGGLRLRCPCH
                     GSTFDAGTGAVTKGPASRPLAEVEITVRGDQIVRG"
     gene            complement(17505..18029)
                     /locus_tag="VLC50_00405"
     CDS             complement(17505..18029)
                     /locus_tag="VLC50_00405"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HSF25955.1"
                     /translation="MDDPRPTHPLIARRTALRGVVGISGAVGLALAGCSSGATTGESP
                     SASAPATGGAGPTGATSGSTDAGSTPAGSGAGPALVAVADVPVGGATVVKADGASYVV
                     AQPSAGTFAVHSAKCTHMGCTVGVDTGLTLECPCHGSRYDAASGKVERGPAPRPLAAA
                     EVTVSGGMVHLVEG"
     gene            complement(18124..>18767)
                     /locus_tag="VLC50_00410"
     CDS             complement(18124..>18767)
                     /locus_tag="VLC50_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018488105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
                     /protein_id="HSF25956.1"
                     /translation="RTVYVPCEVCNGARYNRETLEVHYKGKTIAEVLDMPIDEALEFF
                     AAVPAIARHLGTLNDVGLGYVRLGQPAPTLSGGEAQRVKLASELARRSTGRTVYVLDE
                     PTTGLHFDDIRKLLGVLTGLVDKGNTVVVIEHNLDVIKTADWVVDLGPEGGSGGGLVV
                     AEGTPEQIAVHPTSHTGVYLRHVLDPAAVAAAAGATTSGSASRPRARRRSSTR"
BASE COUNT         2435 a         6970 c         6857 g         2505 t
ORIGIN      
        1 cctgctgttc cagaaggtca tgcgccacga ccccaccgac cccgactggg tggctcgtga
       61 ccgcttcgtc ctctcagccg ggcactcgag cctcaccctc tacatccagc tcttctacgc
      121 cgggctgatc gagctcgacg acctgacccg gctgcgcacc tggggcagcc ggacccccgg
      181 ccaccccgag cacgggcaca ccccgggggt cgagacgacc acgggtccgc tcgggcaggg
      241 catcggcaac gcggtgggca tggccatggc cgcccgccgc gagcgcggcc tgctcgaccc
      301 cgacgccccg gccgggcaga gcgtgttcga ccacaccatc tggtgcgtgg cctccgacgg
      361 cgacatcgag gagggggtca gctccgaggc cagctcgctg gctggcaccc agcggctggg
      421 caacctcgtc gtcatctacg actgcaacaa gatctccatc gaggacgaca ccgacatcgc
      481 cctgtccgag gacaccgcgg cgcgctaccg ggcctacggc tggcacgtcc aggaggtcga
      541 ctggacccac gacgggcacc actacgacga ggacgtcgcg gcgttgcacg ccgcactgct
      601 ggcggcccag gccgagcgcg atcggccgtc gttcatcgtg ctgcgcacga tcatcgcgtg
      661 gccggcgccc aacgcccaga acaccggcaa ggcgcacggc gcggcgctgg gcgccgacga
      721 ggtggccgcg accaaggcgg tgctggggct cgacccgggc gccagcttcg ccgtcgccga
      781 tgacgtgctc gggcacgtgc gtggcgtcat cgagcgcggc cgagccgcgc acgcggcctg
      841 ggcgccgcgg ttcgaggact ggcggcgcag tgcccccgac cgggccgagc tctacgaccg
      901 gatgcgcgta cggcgcctgc cggacgggtg gaccgacgcg ctgccggcgt tcgaggccgg
      961 cgccgacatg gctacccgca aggcgtccgg cacggtgctc tccgcgatcg cggcccacct
     1021 gcccgagctg tggggcggct cggccgacct ggccgagtcc aacaacacca cccccaacgg
     1081 cgagctgagc ttcctgccgc ccgaccgggc gaccaaggcg ttccccggcg actggtacgg
     1141 ccgggtgctg cacttcggca tccgcgagca cgcgatgggc tcgatcctca acggcatcgc
     1201 cctgcacggc ggcacccgcc cctacggcgg caccttcctc gtgttctccg actacatgcg
     1261 cccggcggtc cggctggcgg ccctgatggg cctgccggtg acctacgtct ggacgcacga
     1321 ctcgatcggg ctgggcgagg acggcccgac ccaccagccg gtcgagcacc tggccgcgct
     1381 gcgggcgatc ccaggactcg acgtcgtacg cccggccgac gccaacgaga ccgtcgtggc
     1441 ctggcgcacc gtgctcgagc acaccgaccg accggccggg ctgtgcctga cccggcagaa
     1501 cgtgccgacc ttcgaccggt ccccggccgg cgggttcgcc tccgccgagg gggtcgcccg
     1561 cggcggctac gtcctgctcg acgcgcccga cggccgggtc gacgtggtgc tcgtcggcac
     1621 cggctccgag gtcgccctgg ccgtcgccgc gcgcgaggcg ctcgccggcg agggcgtcgg
     1681 cgcgcgagtg gtgtcgatgc cgtgccggga gtggttcgac gcccagccgg ccgactaccg
     1741 cgactcggtg ctgccgcccg cggtccgcgc ccgggtcagc gtcgaggccg gcgtggcggc
     1801 gggctggcgc gaggtggtcg gcgacgccgg gcgcatcgtc tccttggagc acttcggcgc
     1861 gtcggcggaa taccgggtgc tctaccgcga gttcgggatc actgccgacg ccgtcgtcgc
     1921 ggccgcgcac gagagcctta ccgccaccgg ggccggcacc ggctgacacc caccccacgg
     1981 agggacgacg acatgagcga caccctcgcc gcgctgcacg cccagggcgt gtccatctgg
     2041 ctcgacgacc tgtcccgcga gcggctgcgc tcgggcaccc tggccgcgct ggtgcgcgac
     2101 cggcacgtcg tcggggtcac caccaatccg acgatcttcc agaaggcgct ggccgagggc
     2161 gacgcctacg ccgaccagct ggccgacctg gccgcccgcg gggtcgacgt cggcgaggcg
     2221 atgcgggcgc tgacgaccca cgacgtgcgc tgggcggccg acgtgctgcg ccccgtctac
     2281 gacgcgaccg gcggcctcga cgggcgggtc tcgatcgagg tcgacccgcg gctggcgcac
     2341 aacccgacca gcaccatcgc cgaggcccgg gcgctgtggt ggctcgtcga ccggcccaac
     2401 gcgttcatca agatcccggc caccgaggcc gggctgccgg cgatcagcca ggcgctgtcc
     2461 gagggcatca gcatcaacgt cacgctgatc ttcgggctcg accgctactc cgcggtcatg
     2521 gaggccttcc tcgacggcat ggagcgggcc cgcgccgccg gccgcgatct gtccacgatc
     2581 cgctcggtgg cctcgttctt cgtcagccgg gtcgacaccg aggtcgacaa gcggctggac
     2641 aagctcggcg acccggcggc cgccgcgctg cgcgggcagg ccgcgatcgc caacgctcgg
     2701 ctggcctacc agcgctacga gcaggtgttc acctccgccc gctggcaggc gctgcaggcg
     2761 gccggcgcgc acccgcagcg cccgctgtgg gcgtccaccg gcgtcaagga cccggcctac
     2821 gacgacaccc gctatgtcgt cgacctcgtc accgacggtg tcgtcaacac catgcccgag
     2881 ccgacgctgg atgcggtggc cgaccacggg cggctgcgcg gcgacacggt gcggggcacc
     2941 tacgccgagg ccgcgcaggt cctcgagcag ctcgaggggc tcggcatctc ctacgccgac
     3001 gtcgtcgggg tgctcgagtc cgagggcgtc gagaagttcg aggcctcctg gagccagctc
     3061 gccgaggcgc tggccacctc gctgcagcag acacggagca cccaggggca ccggtgagct
     3121 cggcggtcgc cgtacgcacc gccggggccg ccgccgacgc cgtcagccgc cacctgccca
     3181 cgctggtcgg cgaccgggtg gccagtcggc tcgccgagca ggaccccacg ctgtggggcc
     3241 cggcggtggc agccgacgcc gcggtgcggc tctcgtgggt cgacctggcc cgctcgtcac
     3301 gaccgctcct caccgaggtc gcggcgctgc gcgaccggct gcgcggcctc ggtctcgacc
     3361 acgtggtgct gtgcgggatg ggcggctcgt cgctggcccc ggaggtgatc agccgtacgg
     3421 cgggcgtcga gctcacggtc ctcgactcga ccgaccccga ccaggtgcgc gccgccctgg
     3481 ccgatcggct cgaccgcacc gtggtcgtcg tctcgtcgaa gtcgggaacc acggccgaga
     3541 ccgacgcggg gcggcgggcc tatgagcagg cgttcgcggc ggccggcctc gacccccgag
     3601 agcgcatcgt ggtcgtcacc gacccggggt cggccctcga ggagctggcc ctggcggccg
     3661 gctacgtggt cgtgcgcgcc gacccgcacg tcgggggccg ctactccgcg ctcagcgcgt
     3721 tcggactggt gcccagcggg ctggccggcg ccgacctcgc ccggctgctc gacgaggccg
     3781 aggcggccag gccggcactg gtccgcgacg acccggccaa cccggccctg ctgcttggcg
     3841 cggcgctcgg aggcaccgcg ccgctgcgcc acttcgtggt cctggtcgag gacccggcga
     3901 gcggtcagcg gctcgccggg ttcgccgact gggccgagca gctgcttgcc gagtcgaccg
     3961 gcaaggccgg caccggcctg ctgccggtcg tcgtcgagga cgggcacgcc cccgacgccg
     4021 tagggccctg cccggatgcc ctggtggtcg agctcggccc ggccgagtcc ggccagcagc
     4081 ccttcggcga gccggcctcc gggctccggg tgaccggccc gctcggcgcg cagctgctgc
     4141 tctgggagct ggccaccgcg gtggcggccc gcctcctgcg gatcaacccg ttcgaccagc
     4201 ccgacgtgga gagcgccaag gtcgccgcgc gcggcctgct cgacgaccgg ccggagaccc
     4261 cggcgccggc cttcgtcgag gcgggcatcg aggtccgtgc cagcgcgggg ctgctcgacg
     4321 gcgtgcacga cctgcccggt gcggtcgacg cgctgctcgg ccggctcggc ccgagtggtt
     4381 atctggcggt catggcctac ctcgaccgcc actccgacgc cgccctcacc gacgtgcggg
     4441 cagcgctggc tcgccgtacc cgccgtccgg tcacattcgg gtgggggccg cggttcctgc
     4501 actccacggg ccagttccac aagggtggtc tggcgaccgg cgtcttcctg cagatcaccg
     4561 gcgccccggc cgccgacctg ccggttcccg accggccgtt cacgttcggt gagctgatcg
     4621 ccgcgcaggc gcagggcgac gcccaggtcc tggccgcccg cgggcgaccg gtcctgcggc
     4681 tgcacctcac cgaccgggcc gccggcatcg cccggctgtg cgaggtgctc gcgtgagccc
     4741 ccgcaccggg gccggccgcc cgcgccgcaa cccgctgcgc gacccccgcg acaagcggct
     4801 gccgcgcatc gccggaccct gcagcctggt gatcttcggg gtgaccggcg acctggcgcg
     4861 ccgcaagctg atgccggcgg tctacgacct ggccaacagg ggcctcctgc cgcccggctt
     4921 cgccctggtc gggttcgccc gccgcgactg ggccgacgag gacttcggca aggtcgtcta
     4981 tgacgccgtc cgcgagcacg cccgcacccc gttccgggag tcggtgtggg cccagctggc
     5041 ggagggcatc cggttcgtgc ccggcgagtt cgccgacgac caggcgttcg accggctcgc
     5101 ggccgtggtc gccgacctcg accgggtgcg cggcaccggc ggcaaccacg cgttctacct
     5161 gtcgatcccc ccgaagctgt tccccgtggt gtgcgaccag ctggcccgct ccggcctgtc
     5221 cgcccagcag cgcgactcct ggcggcgggt cgtcgtggag aagccgttcg ggcacgacct
     5281 ggccagcgcg caggcgctca accgggtggt cgaacaggtc ttcccgcacg acgcggtctt
     5341 ccggatcgac cactacctgg gcaaggagac ggtgcagaac ctgctggccc tgcgctttgc
     5401 caaccagctc ttcgagccga tctggaacgc caacaacgtc gaccacgtgc agatcacgat
     5461 ggccgaggac atcggcatcg gcggccgggc cggctactac gacgacatcg gcgccgcccg
     5521 cgacgtgatc cagaaccacc tgctccagct gctcgccctg accgcgatgg aggaaccggt
     5581 ctccttcgac gcccacgacc tgcgtgccga gaaggagaag gtgctctccg ccgtacggct
     5641 gcctcgtgac ctgcgccggt ccaccgcgcg cgggcagtac gccgccgggt ggcagggcgg
     5701 ccagtacgtc ggcggctacc tcgacgagga gggcatcccg gccagctcgc gcaccgagac
     5761 ctacgccgcg atcacgctgg agatcaacac gaggcgctgg gccggcgtgc cgttctacct
     5821 gcgcaccggc aagcggctgg gccggcgggt caccgagatc gccgtggtgt tcaagcgggc
     5881 cccccacctg ccgttcaccc acaccgccac cgaggagctg gggcagaacg ccctcgtcat
     5941 ccgggtacag cccgacgagg gcgtcacgat gcggttcggc tccaaggtgc cgggcaccgc
     6001 gatggaggtc cgcgacgtca cgatggactt cggctacggg cactccttca ccgagtcctc
     6061 ccccgaggcc tacgagcggc tgctgctcga cgtgctgctc ggtgacccgc cgttgttccc
     6121 gcaccaccgg gaggtcgagc tgtcctggga gatcctcgac ccgatcgagg actactgggc
     6181 ctcccgcggc cgccccgagc cctatccggc cggcacctgg gggccggcca gcgccgacgc
     6241 gatgctggcc cgtgacggcc ggatgtggag gcggccatga tcatcgacct gccgagtacg
     6301 acgaccgccg cggtcaacaa ggcgatggtc gagctgcgcg agagcggcgg ggccgtggcg
     6361 ctcggccgcg ttctgacgct ggtcatcgtc accgctgacg gcgaggccga ggacgccatc
     6421 cgggcggcca acgacgccag ccgtgagcac ccctgccgcg tcatcgtgct ggtcaagggc
     6481 acccggcggg gcgggccgcg cctcgacgcg caggtgcggg tgggcggcga cgccggtgcc
     6541 agcgaggtca tcgtgctgcg ggtgcacggc ccgctcgtcg accacggcgc ctcggtcgtc
     6601 atcccgctgc tgctgcccga cgccccggtc gtcgcctggt ggccgggtga gccgccgccc
     6661 aacccggtgg ccgaccccat cggggcgatg gcccaccggc ggatcaccga cgtggccgcc
     6721 tgccacgacc ccgtgcgggc gctggaggac cgtcgcgcga gctatgtgcc cggtgacacc
     6781 gacctggcgt ggagccgcac gaccctgtgg cgcggtctgc tcgcggcggc gctcgaccag
     6841 ccgccgttcg agcgggtcag ccaggcgacc gtggcgggcg gctcggacag cccgagcacc
     6901 gacctgctcg cggcctggct gaccgccgcg ctgcgagtgc cagtgacgag ggagcggacc
     6961 ccggtcggct ccggtctcgt gtcggtgacc ctgacccgcc ccggcgcgcc gatcgacctg
     7021 gtccgcccgg acggccacgt cgccaccctg tccgtggccg gggcgccgga ccggcgcatc
     7081 gcgctgcccc gccgcagcac cagcgagagc ctggccgagg agctgcgccg gctcgacccc
     7141 gacgagatct atgcgctggc cctcaccaag gggttgccgc ggctggcgcg ccgcgccgcc
     7201 cgggccgccg cctcgggcgc gccggcatga cgccgggggc cgacccgacc gccaccggac
     7261 gcgtcgtgcg ggtccacccc gatgccgggc tgctcgcggc cgccgccgcg gcccggctgg
     7321 tgaccgccat gatcgacctt cagcaggccc gtggcggcgt ctcggtcgcc ctcaccggcg
     7381 gtgggctggg gatcgccacg ctgcgcgcgg cggccgactg cccggcccgc gacgcggtcg
     7441 actggtcggc cgtcgacctc tggtggggtg acgagcgcta cctgcccgcg ggccaccccg
     7501 accgcaacga gacccaggcc cgcgccgccc tgctcgaccg ggtggccctt gacccggccc
     7561 gggtgcaccc catgcccgcc agcgacgggc cggagggcga ggacgtcgac acggccgccg
     7621 cgaggcacgc cgtcgagctg gccgagttca ccgccaagcg cgcccagcag gggctggtgc
     7681 cgacgctcga catcgtcctg ctcggcatcg gctcggacgg acacgtcgcc tcgctgttcc
     7741 ccgctcaccc ggcccgcggt gaccgacgag cagtggtcgg ggtccggtcc tcgcccaagc
     7801 ccccgccggt gcgggtctcg ctgacgatgc cgacgctgtg cgccgccacg caggtgtggc
     7861 tgctggccgc cggtgccgcc aaggccactg cggtggcgca gtcgcttgtg gacggtgcgc
     7921 ccgaaccgct gccggccgcc gaggtgcacg gccgtgagcg caccctgtgg ctgctcgacc
     7981 gcgcggcggc gggcagctca gatctggctc agcaggagcg gccggcggct cagtaggggc
     8041 gaccggtggt gcgcagcttg tccagcgcct cagcgaggat ggcctgcccg tcgacgtcgc
     8101 tgcgccgctc tttcacgtag gccaggtggg tcttgtaggg ctcgttgcgc ggcggtgcgg
     8161 ggggagcgtc cttgtcacgg ccggccggga agccgcagcg cgggcagtcc cactcctccg
     8221 gagcctgcac accggcctct tcggcgaagc tgggccgggt ctcgtggccg ttggcgcacc
     8281 agtaggagat ccggaaccgc ggtgccgact cgccccgctc ggcctccccc atgggaccgg
     8341 cgccgactcg gctgccccgg atcgcgttgc cactggccac ggtctgctct cctcactggt
     8401 tcgtgctggt cggaccctgc cgcagaccgg tacgccggcc gacggtgagg ccgacggtca
     8461 ctgcttgaac gacaggccga tcgccacgat ggacgcggtc cagatgatgc cggtgaagat
     8521 cgtgagccgg tcgaggttct tctcgacgac cgccgacccg cccagcccgg aggaggcacc
     8581 gccgccgaac atgtccgaga gcccgccgcc cttgcccttg tgcagcagca ccagggcgat
     8641 catcgcgatg ctcgtgatga tgaggatcgc cgacaggacg tattcaaaga tcgtcacggg
     8701 tggccgctcc ctcgctcagg ccggcggccg ggtgcgggtc ccgaccggag atgtccgggc
     8761 accagggtac gacgcgccgc ggctgctctc tgccaccggg cgctcagccg gtgagctggt
     8821 ggctgcgata acggcagatc gaggtgaact cgtcggggtc gagcgacgcc ccaccgacca
     8881 gcgccccgtc gacgtcgggg cgagccatga tccccgcgac gttgctggcc ttgaccgagc
     8941 cgccgtagag cacccgcacc gcgtcggcga gggcaccgga gtagcgctcg gcgagccgcg
     9001 tgcgcagcgc ggcgcacacc tcctgggcgt cgtcgggtgt ggcgacctca ccggtgccga
     9061 tcgcccagac cggctcatag gccacgacca ggtcgcgggc cgcctcggca ccgacgccgt
     9121 cgagggcacc gtcgagctgg gccagcgtgt gctcgacctg gcgcccctca cgacgcacct
     9181 cgaggccctc ccccacgcac aggatcggag acagcccgtg ccggaaggcc gccttgacct
     9241 tggcgttgac gaggtggtcg tcctcgccgt gataggcgcg ccgctccgag tgccccacca
     9301 cgacataggt gcagccgagc ttagccagca tcgccccgga cacctcgccg gtgtaggccc
     9361 ccgagtcgtg cgcggacacg tcctgggcgc cgtagcggat gtcgagccgg tcaccgtcga
     9421 ccagcgtctg gatcgagcgc aggtcggtga acggggtcag cacggcgacc tcgaccgcgg
     9481 tgaagtcgtg cttcttgtcc ttgagggccc acccgagctt ttggaccagc gccatcgcct
     9541 cgaagtggtt gaggttcatc ttccagttgc ccgccatcag cgggacacgt ccggtgtgcg
     9601 aggactccgc catcgatcag ccctccagga cctgcaggcc gggcaacgtc ttgccctcga
     9661 gatattccag gctcgcgccg ccgccggtgg agatgtggtc gaacgcgtcg tcgctgaaac
     9721 cgagccggcg tacggcggcc gcggagtcgc cgccgccgac cacggtgtag cccttggtcg
     9781 ccgcgagcgc ctcggccacg gcgcgggtgc cggccgcgaa ctcggggatc tcgaacacgc
     9841 ccatcggccc gttccagaac acggtgcgcg actcgcgcac ggcggtggcg aactgcccgg
     9901 ccgactcggg cccgatgtcc acgcccacca tgtcggcggg gatcgcgtca ctggccacca
     9961 cccggtgctc ggcaccgggc tcgaccgcgg ccgccaccac gatgtcgacc ggcagcagga
    10021 tgcgcacgcc gcggcgctcg gccgtctcga ggtagccacg cacggtgtcg acctggtcgg
    10081 cctcgagcag gctgcgcccc acacggtgac cgcgggcggc caggaacgtg aacaccatcc
    10141 cgccgcccac caggatctgg tcggcccgcg agagcagcgc ctcgatgacc gccagcttgt
    10201 cggagacctt ggccccgccg aggaccaccg tgtaagggcg ctccggcgcg ctggtgagcc
    10261 gctgcagcac ctcgacctcg gccccgacca gtccgcccgc ggcgtgcggc aggcgagcgg
    10321 ccacgtcgta gacgctggcg tgccggcggt gcacggcacc gaagccgtcg ctgacgaaaa
    10381 cgtcggcgag cgcggccagc tggtcggcga atgcggctcg ctccgcgtcg tccttgctgg
    10441 tctcgcccgg gttgaagcgc acgttctcga gcagagccac cgcgccgtcg tggagcgcgt
    10501 cgacggccgc gcgcgcgctg ggcccgacgg tgtcggtcgc gaaggcgacc tcgacaccga
    10561 gcagctcgcc gagccggtcg gcgaccgggc gcagcgagta gcgggggtcg ggctggccct
    10621 tcggccggcc caggtgggcc acgaccacca cccgggcccc gcggtcgagc agggtgcgga
    10681 tcgtcggcac cgaggcccgg atccgtccgt cgtcggccac gacgggcgcg ccggccgcgg
    10741 tgtcgagcgg gacgttgagg tcggaccgga ccaggacccg ctgaccccgc aggtcgccga
    10801 ggtcgtcgat cgtcttcacg aggtcgcccc tcgcgcccgc cccggtcaga gggacgagcc
    10861 gacgagggtg accaggtcga cgagccggtt ggagtagccc cactcgttgt cgtaccagcc
    10921 gaccaccttg acctgggcgc cgatgacctt ggtcaggccg gcgtcgaaga tgcaggaggc
    10981 cgggtcgttg acgatgtcgc tggagacgat cgggtcctcg gtgtagtgga tgtagcgctt
    11041 gtaggcgccc tcggcggcgg ccttgaccgc ggcgttgacc tcggcgaccg aggtctcgcg
    11101 ggagacgtcg acggtcaggt cggtgaccga gccggtcggc accgggaccc gcatggcgaa
    11161 gccgtcgagc ttgccgttga gctggggcag gaccagcccg atcgccttgg ccgccccggt
    11221 gccggtggga acgatgttga cggcggcggc ccgggcccgg cgcaggtcct tgtgcgggcc
    11281 gtcctgcagg ttctggtcgg cggtgtaggc gtggatcgtc gtcatgaggc cgcgctcgat
    11341 gccaaacgtg tcgtggatga ccttggccat cggggccagg cagttggtgg tgcatgaggc
    11401 gttggagatg atcgtgtggg cggccgggtc gtagtcggcg tcgttgacgc ccatgacgac
    11461 ggtgaagtcc tcgtgcttgg ccggcgccga gatgatcacc ttggtggcac ccgcctcgac
    11521 atgggcgcgc gccatcgtgg cgtcggtgaa ccggccggtc gactcgagca cgatgtcgac
    11581 gccgagctcc ttccacggca gcgcggtggg gtcgcgctcc tccagggccc ggaccacgtg
    11641 gtcaccgacc cggatgttgc tctcgtcgta ggagacgggc aggccgagcc ggccgagcgt
    11701 ggtgtcgtag gtcagcaggt gggccagcgt cttgttgtcg gtgaggtcgt tgaccccgac
    11761 cacctcgatg tcggcgccgg acgccagcac ggcacggtag aagttgcgtc cgatccggcc
    11821 gaagccgttg ataccaacgc ggatgctcac gaggggtcct cctcggcatg ggccggcctc
    11881 gtggccggct cgacggtcgt cgtagtcacc aggtggtccg cgcgcacagc ctgtcgccgt
    11941 gggcgggccg acctgcttcg accctagggg atcgagcccg gtctccggca ggggacccgg
    12001 tgggccgggc cccgggtcag gcctcgccga gcagctcggg ggtcaggttc gcctcggtgc
    12061 cgggcacccc cagctccgcc gcccgacggt cggccatcgc cagcagccgc cggatgcgcc
    12121 ctgccaccgc gtccttggtc atcggcggct cggagagctg gcccagctcc tcgaggctgg
    12181 cctggccgtg ctccagccgc aggacgccgg cgctgcgcag gtggtcgggc gcctcgtcgc
    12241 cgaggatctc cagggcacgc ttgacccgcg ccgcggcggt caccgcggcc cgggccgagc
    12301 ggcgcaggtt ggcgtcgtcg aagttggcca gccggttggc ggtggcccgg acctcccggc
    12361 gcatccggcg ctcctcccag gccagcacgc tgtcgtgggc gcccagcctg gtcagcatct
    12421 cgccgatggc atccccgtca cggaccacca cgcggtcgac accacgaacc tcgcgggcct
    12481 tggccccgat cgagagccgg cgagccgcgc cgaccagcgc cagggcggcc tcggggccgg
    12541 ggcaggtgac ctcgagcgcg gaggagcggc ctggctcggt tagcgagccg tggacgagga
    12601 acgctcctcg ccaggccgac tcggcgtcgc aggtcgcgcc gctcaccacc tgtggcggca
    12661 gccctcgcac cggtcgacca cgaccgtcga ccagcccggt ctggcgggcc agcgcctcgc
    12721 cgtcccggac gacgcgcacg atgtagcgac ttccccggcg cagcccgccg gcggccacga
    12781 cggtgaactc cgacgggtgg ccgaacacct cggcgatgtt gcgccgcaac cggcgggcgg
    12841 cgacgccggt gtcgagctcg gcctcgacga cgatgcgccc gcccacgagg tgcagcccgc
    12901 cagcgaagcg gagcatcgag gagacctctg ccttgcggca gcacaggcgg gtgatcgtca
    12961 ggcggctgag ctcgtccttc accgaggcgg tcatggccat gcgctagatc ctcccatgat
    13021 gtgccgtcgt gtccgacctc tcgccggtcg gccgcgcgga cgccgtgcca gcgaacgtcg
    13081 cggcgaacgc ggcggccagc cgggtcgggt cgtggcgtgg cgagccgtcg gcggcgctga
    13141 ccgggcggac caccagccgg gcgcccagct cggcggcgac ccgctgcagc ggacccagct
    13201 cggcgaccgt gcgcgggtcg acgatcacga cgtcgagccg caggccgggg gcgtgggcgg
    13261 ccagcacctc gaggtgggtc tccggggaga agccgctcgt ctcccccggc tgcggcgcca
    13321 ggttgagcac gacggcgcgg cgagccgtgg tctcgcacag ggcggcggcc agctcgggca
    13381 ccagcaggtg cgggatgacc gagctgaacc atgaaccggg accgagcacc acccagtcgg
    13441 cgtagcggac ggcggtgacg gcctcggcgc aggccggtgg ctggtcgggc acgagacgca
    13501 gcccggcgac ccggcccggg gtcgacgcca ccgccacctg gccgcgcacc gacgtgaccc
    13561 cgtccgggtc ggcggcgtcg gcgcccacga cggtggcctc caggtcgagc ggcaccgagc
    13621 acatcggcag cacccggccc tccgcgcgca gcagccgccc gacgaggtcg agcgcgggca
    13681 ccgtgtcgcc gagcagctcc cacagcgcga cgatgagcag gttgcccacg gcgtgcccgc
    13741 gcagctcacc gtcgcctcgg aaccggtgct gcacgaggtc gctccaggtg cggccccagt
    13801 cgtcgtcgcc gcacagggcc gccagcgcca tccgcaggtc acccggcggc agcacgccca
    13861 gctcgcgtcg cagccggccg ctggagcccc cgtcgtcggc gaccgtgacg acggcggtga
    13921 gccggtcggt gacccgccgc agcgcggtca gcgaggcggc cagcccgtgg ccgccgccca
    13981 gggcgacgac ggccgggccg ccacgcgggt cggccagcgg ccgactgggg aacgaggcgg
    14041 gcacgcggcc cacgccgggc tcgtcgatgc tcactcgcgg cccaggtcgc gatggaccgt
    14101 gacggcgtcg acgtcgtcgg cgatcagcag cgcggcgagc tgctcggcca tgacgaccga
    14161 ccggtgcttg ccgccggtgc acccgaccgc gatggtgacg aaccgcttgc cctcgcggcg
    14221 atagccctcg atgagcatca gcagcagctc gtgatagcgg gccaggaagt ccttggcgcc
    14281 gacctgggtg agcacgtagt cccgcaccga cgggtcgagc ccggtgcgct ggcgcagctc
    14341 ggggacccag tgggggttgg gcaggaaccg catgtcggcg acgaggtcgg cgtcgaccgg
    14401 gaggccgtac ttgaagccga acgacatgac ggtggcccgc aggtcgggcg tgggctgctc
    14461 ggggaacgcg gcggcgacct tgcgggcgag ctcgtgcacg ttgaggtcgc tggtgtctac
    14521 gacgatgtcg gcgtccccgc gcaggtcgcg cagcatgtcg cgctctcgca cgatcccgtc
    14581 gacgacccgg ccgtctccct gcagcgggtg cgggcggcgc accgcctcga agcgccggac
    14641 cagggcgtcg tcgttggcct cgaggaagag cacccgcggg tcgctgccgg ttcgggccag
    14701 ctcgtcgatc gcttcccgca gggcggcgaa gaacgaccgg ctgcgtacgt cgaccaccgc
    14761 ggcgatccgg gcgaccgcac cctgggtgcg tccgcccagg tcggccagca gcggcagcag
    14821 ctcgggcggc aggttgtcga cgacgtacca gccaaggtcc tcgagcacgt tggcggccgt
    14881 ggtgcgcccg gcgcctgaca tgccggtcac gatgatcagc tgcggtgcgt ggacgcgccc
    14941 gtcggcgacg tcggagatgt tcactgcggg tccccccggt ctcgcggcgc ggtggtcggc
    15001 gtcggatcgg tcgtggcgtc gaggatctca cccgtgccca cgtcgacggc cggactcggg
    15061 tgatcagctg ccagcgcgat cacgacggcc gcggcggtgc gcgggccgat cccgggcacc
    15121 gcggcgatct cctcggccgt ggcggcccgt agccgcttga gcgagccgaa gcgggtcatc
    15181 aacgccttgc gccgggtctg ccccaggccc gggatcccgt cgagcgtgct ctcgagcatc
    15241 gccttgcgtc ggcgctgccg gtggaacgtg atggcaaacc ggtgcgcctc gtcacgcacc
    15301 cgttggagca ggtagagccc ctcgctcgag cgaggcagca cgaccgggtg ggcctgcccc
    15361 ggccgccaca cctcctcgag ccgcttggcc agcccgacga gcacgacgtc gtcgatcccg
    15421 aggtcgttga gggcgcgctg gccggcggcc acctgcggcg ggccaccgtc gaccacgacg
    15481 aggttgggcg ggtaggcgaa ccgtcgcgac tgcccggcct cggggtcctc gaggaggtcg
    15541 agcggcgccg gccggtcgcg gtgctcgagg tagcggcgga accgacgggt gatcacctcg
    15601 tgcatcgacg cgacgtcgtc ctgcccggcc accgtcttga tcgtgaaccg gcggtactcg
    15661 ctcttgcggg ccagcccgtc ctcgaacacg accatcgacg cgacgacctg gtcggcaccg
    15721 aggttggaca cgtcgaagca ctcgatccgc aacggcgcct gggtcagctc gagagcgtct
    15781 tggatctcct gcagcgcctg gctgcgggtg gtcaggtcgc cggcccgccg caccttgtgc
    15841 agccccagcg cctgggtcgc gttgcgggcg accgtctcga gcagggcccg cttgtcgccc
    15901 cgctgcggta cccggaccac gacgcgcgag ccgcgcaggc cgcgcagcca ctcctccacc
    15961 gccgcgacgt ccggcggcag ctccggcacg agcacctctc ggggcaccga gtcggcccag
    16021 ccgtcgccgt tggcgccgta gagctgctgg aggaagtgct cgatgaggtc ggcggtggtg
    16081 aggtcctcca ctttctcgac cacccaccct cgctgcccgc ggatgcgtcc cccgcgcacg
    16141 tagaacacct ggaccgccgc ctcgagcggg tcctcggcca cggcgacgat gtcggcgtcc
    16201 gtgccgtcgc cgagcacgac ggcgttcttc tccatcgccc gctcgagcgc cttgacgtcg
    16261 tcacgcagcc gcgccgcccg ctcgtagtcg aggttggccg cggcgacctg catctccttc
    16321 tcgaggcggc gcacgtaggt cgacgtctgc cccgccatga agtcgcagaa gtcctccacg
    16381 atcgcccggt gctcctcggc gctgacgcgg cccacgcagg gcgccgagca cttgccgatg
    16441 tagcccagca ggcagggccg gccggcccgg ttggctcgct tgaacacccc ggcggagcac
    16501 gtgcgggcgg ggaacacccg caacagcagg tcgagggtct cccggatcgc ccaggcgtgg
    16561 gcgtagggcc cgaagtagcg ggtgcccttg cgcttggcgc cgcgcatcac ctgcaaccgg
    16621 gggaactcct cggacagcgt caccgccaga taggggtagg acttgtcgtc gcggtagcgg
    16681 acgttgaacc gcgggtcgaa ctccttgatc caggaatact caagggccaa cgcctccacc
    16741 tcggtgccca ccacggtcca ctcgacgctg gcggccgagg tgaccatggt ctgggtgcgc
    16801 gggtgcaggc ccgacaggtc ggcgaagtac gacgacagcc gctgacgcag gctcttggcc
    16861 ttgccgacgt agatgacccg gcggtcggcg tcccggaagc ggtagacccc gggttcgtcg
    16921 ggaatcgacc gagtcgccgg ccgatagctc gcggggtctg ccacggcaat caccctacgt
    16981 cgatcgggcc gaaccacccc gtgggcgatc ccgctcggcc gtcgcggggc tcagccccgc
    17041 acgatttggt caccgcgcac ggtgatctcc acctcggcga gcggtcggct ggccgggccc
    17101 ttggtcaccg cgccggtgcc agcgtcgaag gtgctgccgt ggcacgggca gcgcaggcgg
    17161 agaccgccat cggtgccgac ggtgcacccg ccgtgggtgc agatcgcgct gtggccgatg
    17221 aactggccct gggtgggctg ggcgatcacg acgggttgcg agccgccgat gactttggcc
    17281 tcaccgaccg ggacgtctgc gatcgcggtc agctccgtcc ccggggcggc agaggggccg
    17341 ccgccggcgc agcccgccag gccggcggcc gccaccccca tcgccgccgt gccccgcagc
    17401 agcgtgcggc gggtgatggg gtgtgcggtg ctgccctgct ccactgggcc acctccgtgg
    17461 gtcgtcgcgg tcgggaaccg acaacgctac gtggtgaccg cggctcagcc ctcgacgagg
    17521 tgcaccatcc cgccgctgac ggtgacctcg gcggcggcca gcggccgggg cgccggtcct
    17581 ctctccacct tgccgctggc cgcgtcgtag cgactgccgt ggcaggggca ctcgagagtg
    17641 agcccggtgt ccacaccgac ggtgcacccc atgtgggtgc acttggcgct gtgcaccgcg
    17701 aaggtccccg cactcggctg ggcgacgacg tagctggccc cgtcggcctt gacgaccgtg
    17761 gcgcctccca ccggcacgtc ggccaccgcg acgagtgcgg ggccggcgcc ggagccggca
    17821 ggcgtcgacc cagcgtccgt ggacccgctg gtcgcccccg tgggccctgc tccacccgtc
    17881 gccggtgcgc tcgccgaggg cgattctccg gtcgtcgcac ctgagctgca gccggccagc
    17941 gcgagcccga cggctcccga gatcccgacg acgccgcgca gcgcggtccg tctggcgatc
    18001 agcgggtggg tgggtcgcgg gtcgtccatc gtcgctccct caggtggccg gacaggcgtg
    18061 acggcgctgc cgtcacgcct ggtgcaacgc ggcgagtggc cgaccggttc gacgaccggc
    18121 ggactagcgg gtgctggaac gacgccgagc cctcgggcgc gaggccgacc cagaggtggt
    18181 tgcgccggcg gccgccgcga cggcggcagg gtcgaggacg tggcgcaggt agacgccggt
    18241 gtggctggtc ggatggaccg cgatctgctc gggggtgccc tcggcgacca cgagcccacc
    18301 gcccgagccc ccctcgggac cgaggtcgac gacccagtcg gccgtcttga tgacgtcgag
    18361 gttgtgctcg atgaccacca ccgtgttgcc cttgtcgacc aggccggtga gcacgccgag
    18421 cagcttgcgg atgtcgtcga agtgcagccc ggtggtcggc tcgtcgagga catagacggt
    18481 ccggccggtc gatcggcggg ccagctcgga ggccagcttg acccgctggg cctcgccgcc
    18541 ggacaacgtg ggggcgggct ggcccagccg gacgtagccg agcccgacgt cgttgagcgt
    18601 gcccaggtgc cgggcgatgg ccggcaccgc ggcgaagaac tcgagcgcct cgtcgatcgg
    18661 catgtcgagg acctcggcga tcgtcttgcc cttgtagtgc acctcaagcg tctcgcggtt
    18721 gtagcgggcg ccgttgcaga cctcgcacgg gacgtagacg gtccggc
//
LOCUS       DATIBB010000012         8884 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_531833, whole genome shotgun sequence.
ACCESSION   DATIBB010000012 DATIBB010000000
VERSION     DATIBB010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 8884)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8884)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8884
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_531833"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..232)
                     /locus_tag="VLC50_00415"
     CDS             complement(<1..232)
                     /locus_tag="VLC50_00415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF25957.1"
                     /translation="MVERRDIASWLSGPRAALEGTDAELGYRGQRLGRPESGAGAVAG
                     FGVRLLALMIDWTAALLVVHLFWPDLAYGSPDY"
     gene            419..1843
                     /gene="glnA"
                     /locus_tag="VLC50_00420"
     CDS             419..1843
                     /gene="glnA"
                     /locus_tag="VLC50_00420"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HSF25958.1"
                     /translation="MFSSSEEALEFIRDEGVRFIDVRFCDLPGVMQHFNAPVEYFDAD
                     TFAEGLMFDGSSIRGFQAIHESDMKLVPDVRTAFVDPFRKQKTLALNFSIVDPFTDEP
                     YSRDPRNIAAKAEAHLAQTGIADTAYFGAEAEFYVFDDVRFETRANAGYYYIDSIEGA
                     WNTGRAEEGGNRGYKTRYKGGYFPVPPVDHFADLRDEMVTTLQQVGLKVERSHHEVGT
                     AGQAEINYKFDSLQHSGDQMMLFKYVIKNVAWANGRTATFMPKPLFGDNGSGMHAHQS
                     LWKDGVPLFYDELGYAGLSDLARWYIGGLIKHADSLLAFTNPTVNSYHRLVPGFEAPV
                     NLVYSQRNRSAAVRIPVTGTSPKAKRLEFRVPDPSCNPYLAFSAMLMAGLDGIKNKVE
                     PPEPIDKDLYELPPDEHAQVKQVPDSLPAVLDALERDQDYLLEGNVFTPDLIETWIDW
                     KRTNEVDPIRLRPHPHEFELYFDL"
     gene            2000..2377
                     /locus_tag="VLC50_00425"
     CDS             2000..2377
                     /locus_tag="VLC50_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HSF25959.1"
                     /translation="MPKPVRVIRTEELVDADPTPGMHRLQAVDVPGLWSGVVHTEPGA
                     TSGWHHHGQHETSLYVVSGAMRLEFGSGGQAAVEAGPGDFVHVPAMAIHRESNPTNHT
                     ATAIIARAGTGVPTVNVDGPEDA"
     gene            2550..4286
                     /locus_tag="VLC50_00430"
     CDS             2550..4286
                     /locus_tag="VLC50_00430"
                     /inference="COORDINATES: protein motif:HMM:NF019352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase family protein"
                     /protein_id="HSF25960.1"
                     /translation="MTNPDLFTTTTEGLAEVAPPTLVRLRDGDRFDLRITPVRKDLDD
                     GSRLRMLAYNGSIPGPTLHVDQGSTITVHVANEGDVETTVHWHGLRLENRFDGVPHET
                     QEPIPVGGTFSYQIQFPDPGFYWYHPHLREDFAQEMGLYGCIVVEPSDSSYWSPVDRQ
                     LTLTLDDLLVEGGKVAPFRKSGPTFTAMGRFGNVLLVNGQTEFTGEAVAGEVVRLHLV
                     NAANTRLFNVGLRGARAKLVGGDSGRYERETFVDEVLLAPSERAILDVLFEAAGQVRL
                     EHRTPDQVYDLGTFTVASPSGSTGSGAEAGAAARSFPALRVDPELSAERRALAGDLER
                     APDKVLAFVSHMPLLYGDDQSAAASLFACPMHPEVTATEPATCPKCGMKLVPADADAA
                     HAGHEAHAGHEGHAEHEAHAEHEAHAEHEPPAGHEAHAGHEGHAGGDGLEWEDLMPAV
                     NRASDPSNMIWELVDRATGAANTAITWAFTVGDRVKIRLVNEMDSDHPMHHPFHIHGA
                     GRFLVLARDGAPEPNLVWKDTVLLRAGETVDILLDVSNPGLWMAHCHIAEHTESGMMF
                     SFDVARRPEPVR"
     gene            4283..5332
                     /locus_tag="VLC50_00435"
     CDS             4283..5332
                     /locus_tag="VLC50_00435"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013678379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HSF25961.1"
                     /translation="MTAAPRDVLVIGGGQAGLAMGHELARRGARFEIVDAGAEVGGSW
                     RSRWDSLRLFTAARFDGLPGLPFPAHPDSYPGKDAVADYLQHYAATFELPVRLNTPVA
                     ALTRFDGGYVAKAGDQELEARHVVVATGPFHLPFVPRLAEGLDPAVHQLHSTDYRRPG
                     ALPPGRTLVVGAANTGCQIAEELAATREVDLSVGARQPTVPQRPLGRDIWWWATLLRL
                     DRVTADSRLGQRLAGRDQNIGVGPRQLARHGVRLRGRAVRAAGKTVTFADGAAADYDV
                     VVWATGFTTDFRWIDEPSLTDEHGRVRHHRGVTAVSGLYLLGQTWQHTRGSALLGWVG
                     NDAAYLADQITRTHR"
     gene            5329..5793
                     /locus_tag="VLC50_00440"
     CDS             5329..5793
                     /locus_tag="VLC50_00440"
                     /inference="COORDINATES: protein motif:HMM:NF039957.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6010 family protein"
                     /protein_id="HSF25962.1"
                     /translation="MNELMNRARQRMRGRDDTQSNGDLRASAVALAGGAALGLVSNAL
                     LALVPREKARLLAGLTLAVAGGAYLGFGVADGRGHALAAQSGEFLAFGAAAYLGVQRQ
                     SSALLGAGWLAHAAWDALHTREGGPTRVPSWYPPACGAYDAAVGVALLKGRR"
     gene            complement(5952..7235)
                     /locus_tag="VLC50_00445"
     CDS             complement(5952..7235)
                     /locus_tag="VLC50_00445"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSF25963.1"
                     /translation="MATDELAADAPPAPVGYSVPLRIGEFRGLISAQVLSELGDQVAR
                     VALASLILIRSGSVFLMAATFAVSFLPAVLGSALLGSLADRLPRKAILLSCDLIRAVL
                     IGAIALLATDEAPLVLLLGLLFVAELFSAPFLAAQQALLPDVLPEPRDYLAGAGLVRV
                     LSQFDQVLGLVFAGLVVHLLSVRTALVLDALSFAVSFLVLLATLRRRPAAVVDASPGV
                     RGYLRDLGHGARITAADPVRRVLVLLGWGAAFVLIAPEAVALAYARDRGLSELAGSLL
                     MATVPAGAALGAYLIGRIDPVRAVQLLLTLVALACLPLIATMADPPVLIAGLLWLISG
                     TCQGFMVPLVTTVNLATPREQRGMVNGLAAAGFNISTTAAFLVVGALSDLTTPAIAVG
                     AAGIFGLVVVFASRSVWPRRAVRRAARMSYADHPH"
     gene            complement(7251..>8884)
                     /locus_tag="VLC50_00450"
     CDS             complement(7251..>8884)
                     /locus_tag="VLC50_00450"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006606005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional [glutamine synthetase]
                     adenylyltransferase/[glutamine
                     synthetase]-adenylyl-L-tyrosine phosphorylase"
                     /protein_id="HSF25964.1"
                     /translation="SLGYKPDHRGEAVDVLRAELALHTREVRRLHEKLFYRPLLLAVA
                     HLAPDEVRLTPAEASARLGALGYADPDGALRHIEALTSGVSRRAAIQRTLLPVMLGWF
                     ADAPDPDGGLLAFRRVSEALGSTHWYLRLLRDEGQTAERMAHLLATSRLVADLLLRAP
                     EAVAMLADDDELTPRDPAGLLGEVLAGVGRSDDPVEAIGAVRAVRRRELFRVAVADLL
                     GRLEVAAVGAALTDVAVATLVGAFEAARRAVEAERGAPLPTRLAVIGMGRLGGAEMGY
                     GSDADVLFVHEPADGVDEPEAAAAALAVATELRRLLALPSAEPALTVDADLRPEGRSG
                     PLVRTLDSYAAYYARWALPWERQALLRAAPVAGDAELGRRFVELIDPLRFPAEGLEPA
                     AVREIRRVKARVEAERLPRGADPSTHTKLGRGGLADVEWTVQLLQLCHAGAVDGLRTT
                     GTLPALRAAEAAGLAAPADVAALEAAWQLASRVRNAITLVRGKPADSLPNDVRELRAV
                     AAVVGYSPGTTGLIVDDYRRVTRRARTVVDRLFYS"
BASE COUNT         1402 a         3253 c         3117 g         1112 t
ORIGIN      
        1 agtagtcagg gctgccatag gcgaggtcgg gccagaacag gtggaccacc agcagcgcgg
       61 cggtccagtc gatcatcagc gccagcagcc ggaccccgaa accggccacc gcgccggcac
      121 ccgattcggg gcgcccgagg cgctggccgc ggtaaccgag ctccgcgtcg gtgccctcca
      181 gggccgcccg cggacccgac aaccaggacg cgatgtctcg acgctcgacc acgcaacgag
      241 ccgaacgtcc gcgccagagc gcccgtaaca cgctggaaac acccgagaca ccgacgggaa
      301 acgccgagcc tctagcgtcg tcgcgtcagc cggggacagg gtggtcaccg gccggtacgg
      361 gtcatgcacg cggcgacgta cgccgacccg gtctggcagc tccccgcgag gaggatggat
      421 gttcagcagc tccgaagagg ccctcgagtt catcagggac gagggcgtcc ggttcatcga
      481 cgtacgcttc tgcgacctgc cgggggtcat gcagcacttc aacgccccgg tcgaatactt
      541 cgacgccgac acgttcgccg agggcctgat gttcgacggg tcctcgatcc gcggcttcca
      601 ggcgatccac gagtcggaca tgaagctggt gcccgacgtg cgcaccgcgt tcgtcgaccc
      661 gttccgcaag cagaagacgc tggcgctgaa cttctccatc gtcgacccgt tcaccgacga
      721 gccctacagc cgcgacccgc gcaacatcgc ggccaaggcc gaggcgcacc tggcccagac
      781 cggcatcgcc gacaccgcct acttcggcgc cgaggccgag ttctacgtct tcgacgacgt
      841 gcgcttcgag acccgggcca acgccggcta ctactacatc gactcgatcg agggggcctg
      901 gaacaccggg cgggccgagg agggcggcaa ccggggttac aagacccgct acaagggcgg
      961 ctacttcccg gtgccaccgg tcgaccactt cgccgacctt cgcgacgaga tggtcaccac
     1021 gctgcagcag gtcgggctga aggtggagcg ctcgcaccac gaggtcggta cggcgggaca
     1081 ggccgagatc aactacaagt tcgacagcct gcagcactcc ggcgaccaga tgatgctgtt
     1141 caagtacgtc atcaagaacg tggcctgggc caacgggcgt acggcgacct tcatgcccaa
     1201 gccgctgttc ggcgacaacg gctccggcat gcacgcccac cagtcgctgt ggaaggacgg
     1261 cgtgccgctg ttctacgacg agctcggtta cgccggcctg tccgacctgg cccgctggta
     1321 catcggcggg ctgatcaagc acgccgacag cctgctggcg ttcaccaacc cgacggtcaa
     1381 ctcctaccac cggctggtgc ccgggtttga ggcaccggtc aacctcgtct actcgcagcg
     1441 caaccgctcg gccgccgtac gcatcccggt gaccggcacg tcgcccaagg ccaagcgact
     1501 cgagttccga gtgcccgacc cgtcgtgcaa cccctacctg gcgttctcgg cgatgctcat
     1561 ggccggtctc gacgggatca agaacaaggt cgagccgccc gagccgatcg acaaggacct
     1621 ctacgagctg ccacccgacg agcacgccca ggtcaagcag gtgcccgact cgctgcccgc
     1681 ggtgctcgac gcgctcgagc gtgaccagga ctacctgctc gagggcaacg tgttcacgcc
     1741 cgacctgatc gagacctgga tcgactggaa gcgcaccaac gaggtcgacc cgatccggct
     1801 gcgcccgcac ccgcacgagt tcgagctcta cttcgacctc taagccggat ctgccggcat
     1861 tcctgcagta gcgaacgaaa agcgcccggc ccggcggaaa cgtcccttga cgttgaccaa
     1921 cgggacgggc gcttttcgac gttcgtcatc ggaactgggc accttgaccc cagcttggag
     1981 ggctggcgag actcgttgca tgcccaagcc ggtccgcgtc atccgcactg aagagctcgt
     2041 tgacgctgat ccgaccccag gaatgcaccg actccaagcc gtcgatgttc ccgggctgtg
     2101 gtctggggtc gtgcacaccg agcccggagc gacatctggc tggcatcatc acggtcagca
     2161 cgagaccagc ctctacgtcg tatccggggc catgcgcctg gagttcggct caggcggcca
     2221 ggctgcggtg gaggctgggc ccggtgactt cgtgcacgtg ccagcgatgg ccatccaccg
     2281 ggaatccaat cccaccaacc acacggccac cgcgatcatc gcgcgggccg gcaccggggt
     2341 accgacggtc aacgtcgacg gacccgaaga cgcctgaccg gccctccgtt gccgaggtcg
     2401 tagacggtcc ggcacaccgt gcctacggtg aagttgaggc agtcgtggcg tgccgctgca
     2461 cgggctcgaa gactccatga caccacgacc agcgcgggag gccgccggtc ggcccaatcg
     2521 gcggcggccc gcgagggaag aggccgacga tgacgaaccc agacctgttc accacgacaa
     2581 cggagggact ggctgaggta gcgcccccga cgctggttcg gctgcgtgac ggggatcggt
     2641 tcgaccttcg gatcactccc gtgcgcaagg acctcgacga cggctcccgg ctgcggatgc
     2701 tggcctacaa cgggtcgatc cctggcccga ccctgcacgt cgaccaggga tcgacgatca
     2761 cggtccacgt cgccaacgag ggcgacgtgg agaccaccgt gcactggcac ggactgcggc
     2821 tggaaaaccg cttcgacggg gtgccgcacg agacccagga gccgatcccg gtcggcggca
     2881 cgttcagcta ccagatccag ttccccgacc ccgggttcta ctggtatcac ccgcacctgc
     2941 gcgaagactt cgcgcaggag atgggtctgt acggctgcat cgtcgtcgag ccgagcgatt
     3001 cgtcgtactg gtctccagtt gaccggcagc tcaccctcac cctggacgac ctgctcgtcg
     3061 agggcgggaa ggtcgcgccg ttccgcaagt ccgggcccac gttcaccgcg atgggccggt
     3121 tcggcaacgt cctgctggtc aacggccaga ccgagttcac cggcgaggcg gtcgcaggcg
     3181 aggtcgtgcg gctgcacctg gtcaacgccg cgaacacgcg gctgttcaat gtcgggctgc
     3241 gcggtgcccg cgccaagctg gtcggcgggg acagcggccg atatgagcgg gagacgtttg
     3301 tcgacgaggt gctgctggcc ccgtcggagc gggccatcct cgacgtgctc ttcgaagccg
     3361 cgggccaggt gaggctcgag caccgcacac cggaccaggt ctacgacctc ggcaccttca
     3421 ccgtcgcctc cccctcgggc tcaaccggct cgggagcgga ggccggcgcg gccgcccggt
     3481 cgttcccagc gctgcgcgtc gaccccgagc tcagcgccga gcgccgggcg ctcgcgggcg
     3541 atctggagcg ggcaccggac aaggtgctcg ccttcgtcag ccacatgccg ctgctctacg
     3601 gcgacgacca gtcggcggcc gcctccctct tcgcctgtcc gatgcacccc gaggtgacgg
     3661 ccaccgagcc cgcgacctgc cccaagtgcg ggatgaagct cgtccccgcc gacgccgatg
     3721 cggcgcacgc aggacatgaa gcgcacgccg ggcacgaggg gcacgcagag cacgaggcgc
     3781 atgcagagca cgaggcgcat gcagagcacg agccgcctgc agggcacgag gcgcatgcgg
     3841 gacacgaggg gcacgcaggc ggcgacggcc tggagtggga agacctgatg ccggccgtca
     3901 accgggcgtc cgacccgagc aacatgatct gggagctcgt cgaccgggcg accggggcgg
     3961 ccaacaccgc catcacgtgg gcgttcaccg tcggcgaccg ggtgaagatc cgactggtca
     4021 acgagatgga ctccgaccac ccgatgcacc acccgttcca catccacggc gccggccgct
     4081 tcctcgtcct ggcccgcgac ggggccccgg aacccaacct ggtgtggaag gacaccgtgc
     4141 tgctgcgggc cggcgagacc gtggacatcc tgctcgatgt gtccaacccc gggctgtgga
     4201 tggcccactg ccacatcgcc gagcacaccg agagcggcat gatgttcagc ttcgacgtgg
     4261 cccgccggcc ggagccggtc cgatgacggc ggcgccccgc gacgtgctgg tcatcggcgg
     4321 cgggcaggcc ggcctcgcca tgggccacga gctggcgcgc cgcggcgcac ggttcgagat
     4381 cgtggacgcc ggcgccgagg tcggcggctc gtggcggtcc cggtgggact cgctgcggct
     4441 gttcaccgcg gcccgtttcg acggcctgcc cggcctgccg ttcccggcac accccgacag
     4501 ctacccggga aaggacgccg tcgcggacta cctgcagcac tacgccgcca cgttcgagct
     4561 gccggtgcgg ctgaacacgc cggtggcggc gttgacccgc ttcgacggcg ggtacgtcgc
     4621 aaaggccggc gaccaggagc tcgaggctcg acacgtggtc gtggccaccg gtccgttcca
     4681 cctgccgttc gtgccgcggc tcgccgaggg cctcgacccg gcggtgcacc agctccacag
     4741 caccgactac cggcgacccg gcgcactgcc acccggaagg acgctggtgg tcggcgccgc
     4801 aaacaccggc tgtcagatcg ccgaagagct cgccgcgacc cgcgaggtgg acctgtcggt
     4861 cggcgcgcgc cagccgaccg tcccacagcg gccgctgggc cgggacatct ggtggtgggc
     4921 caccctgctg cggctcgacc gcgtcaccgc cgactcccgg ctgggccaac ggctggccgg
     4981 gcgggaccag aacatcggcg tcggcccgag acagctcgct cgccacggcg tccggctacg
     5041 aggccgggcc gtcagagccg caggcaagac ggtgactttc gccgatggcg ccgcggccga
     5101 ctacgacgtg gtcgtctggg ccacggggtt caccaccgac ttcaggtgga tcgacgagcc
     5161 cagcctgacc gacgagcacg gccgggttcg gcaccaccgc ggcgtcaccg ccgtatccgg
     5221 tctctacctg ctcggccaga cctggcagca cacccgcggt tccgcgctgc tcggctgggt
     5281 cgggaacgac gccgcctacc tcgccgacca gatcacaagg acgcaccgat gaacgagctg
     5341 atgaaccggg cgcgccagcg gatgcgcggt cgggacgaca cacagtcgaa cggcgacctg
     5401 cgggcctctg ccgtggctct ggctggcggc gccgcactcg ggctggtcag caacgcgctg
     5461 ctggccctcg tgccccgtga gaaggcccgc ctgcttgccg gcctgacgct ggccgtggcc
     5521 ggcggcgcct acctgggatt cggcgtcgcc gacggccgcg ggcatgccct ggccgcccag
     5581 tccggggagt tcctcgcgtt cggggccgcg gcctacctcg gtgtccagcg tcagtcgtcg
     5641 gcgctgctcg gcgcgggctg gctcgcacac gccgcgtggg acgcgctgca cacccgtgaa
     5701 ggcggcccca cccgggtgcc gtcctggtac ccgccggcct gcggcgccta cgacgccgcg
     5761 gtcggggtgg cgctgctcaa gggccggcgc tagcggggtc agccccctgc caggcggatt
     5821 gcggcgactc ctgcgccccc ttggcaaggc gttcgctcac ttggcagggc gtgcacgcct
     5881 tgccaagtgg caggacgcct acttggcgtg gtcattcgcg ccggggtaac ggttcgcgac
     5941 gagaggtgag gtcagtgggg gtggtcggcg taggacatcc gggcggctcg gcgtacggcg
     6001 cggcgcggcc acaccgagcg cgacgcgaag acgacgacga ggccgaagat gccggcggcc
     6061 ccgaccgcga tcgccggggt cgtcaggtcg gacagcgcgc cgacgacgag gaaggcggcg
     6121 gtcgtcgaga tgttgaagcc ggcggcggcc agcccgttga ccatgccgcg ctgctcccgc
     6181 ggggtggcca ggttgacggt ggtgaccagc ggcaccatga agccctggca ggtcccgctg
     6241 atcagccaca gcagcccggc gatcagcacc ggtgggtcgg ccatggtcgc gatgagcggc
     6301 aggcaggcca gggcgaccag ggtcagcagc agctgtacgg cgcgcaccgg atcgatccgg
     6361 ccgatcaggt aggcccccag cgcggcgccg gccggcacgg tggccatcag caggctgccg
     6421 gccagctcgg acagcccccg atcgcgggcg taggccaggg ccaccgcctc cggcgcgatg
     6481 agcacgaacg ccgcgcccca gccgagcagc acgagcaccc ggcggaccgg gtcggcggcg
     6541 gtgatgcggg cgccgtggcc caggtcgcgc agatagccgc gcacccctgg cgacgcgtcg
     6601 acgaccgcag ccggccgacg ccgcagcgtg gccagcagca cgaggaagga caccgcgaag
     6661 ctcaacgcgt cgagcaccag cgcggtgcgc accgagagca ggtgcaccac cagcccggcg
     6721 aagaccagcc cgagcacctg gtcgaactgg ctgagcaccc ggaccagccc ggccccggcg
     6781 aggtagtcgc gcggctcggg gagcacgtcg ggcagcagcg cctgctgggc cgccaggaac
     6841 ggcgcggaga acagctcggc gacgaacagc aggcccagca gcagcaccaa cggcgcctcg
     6901 tcggtggcca gcagcgcgat cgccccgatc agcacggccc ggatcaggtc gcagctgagc
     6961 aggatcgcct tgcggggcaa ccggtcggcc agcgagccga gcagcgccga gccgagcacc
     7021 gccggcagga agctgacggc gaaggtggcc gccatcagga agacgctgcc ggaacggatg
     7081 aggatcagcg aggccagcgc cacccgggcg acctggtcgc cgagctcgct gagcacctgg
     7141 gccgagatca ggccgcggaa ctccccgatc cgcagcggca cggagtagcc gaccggtgcc
     7201 ggcggcgcat ccgccgccag ctcgtccgtg gccacgcatt cgcccccggg tcaggagtag
     7261 aagagacggt cgacgacggt gcgggcgcgc cgggtcacgc gccggtagtc atcgacgatt
     7321 agtcccgttg tgcccggact gtagcccacc accgcggcta cagcgcgtag ctctcggaca
     7381 tcgttcggta acgaatccgc gggcttgccg cgcaccagcg tgatcgcgtt gcggacccgg
     7441 ctggccagct gccaggccgc ttccagcgcc gccacgtcgg cgggcgcggc cagccccgcg
     7501 gcctcggccg cgcgcagcgc cggcagcgtg ccggtagtcc gcagtccatc gactgccccg
     7561 gcgtggcaga gctgcagcag ctgcaccgtc cactccacgt cggccagccc gccccggccc
     7621 agcttggtgt gcgtgctcgg gtcggctccg cgcggcagcc gctcggcctc cacccgggcc
     7681 ttgacccggc ggatctcgcg caccgcggcc ggctcgaggc cctcggccgg gaagcgcaac
     7741 gggtcgatga gctcgacgaa acgccggccc agctcggcgt caccggccac cggggccgcc
     7801 cgcagcagcg cctggcgctc ccagggcagc gcccaccggg cgtagtaggc cgcgtaggag
     7861 tcgagcgtgc ggaccagcgg accggagcga ccctccggac gcaggtcggc gtccaccgtg
     7921 agcgccggct cggccgaggg cagcgccagc agccggcgca gctcggtggc cacggccagc
     7981 gcggcggcgg cggcctccgg ctcgtcgacc ccgtcggccg gctcatggac gaacagcacg
     8041 tcggcgtcgc tgccgtagcc catctcggcc ccgccgagcc ggcccatccc gatcaccgcg
     8101 agccgggtcg gcagcggggc gccccgctcg gcctcgaccg cccggcgggc cgcctcgaac
     8161 gccccgacga gcgtggccac cgccacgtcg gtcagcgccg ccccgaccgc ggcgacctcg
     8221 agccggccca gcaggtcggc gacggcgacc cggaacagct cccggcggcg tacggcgcgc
     8281 accgcaccga tcgcctcgac cgggtcgtcg ctgcgcccca ccccggcgag cacctcgccg
     8341 agcaggccgg ccgggtcgcg cggggtcagc tcgtcgtcgt cggcgagcat cgccacggcc
     8401 tccggcgccc gcagcagcag gtcggcgacc agccgactgg tggcgagcaa gtgggccatc
     8461 cgctcggccg tctgcccctc gtcgcgcagc agccgcaggt accagtgggt ggacccgagc
     8521 gcctcgctga cccggcggaa ggccagcagc ccgccgtcag ggtcgggcgc gtcggcgaac
     8581 cagccgagca tcaccggcag caaggtgcgc tggatggccg cccggcggct gactcccgag
     8641 gtgagcgcct cgatgtggcg cagggccccg tcggggtcgg cgtagccgag ggcgcccagt
     8701 cgggccgagg cctccgcagg ggtgagccgg acctcgtccg gtgccaggtg cgccacggcc
     8761 agcagcagcg ggcggtagaa gagcttctcg tgcagccggc gcacctcgcg ggtgtgcagg
     8821 gcgagctccg cacgcagcac gtcgacggcc tcgccccggt ggtcgggctt gtaccccagc
     8881 gacc
//
LOCUS       DATIBB010000013         4011 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_795264, whole genome shotgun sequence.
ACCESSION   DATIBB010000013 DATIBB010000000
VERSION     DATIBB010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 4011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4011
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_795264"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(7..1110)
                     /locus_tag="VLC50_00455"
     CDS             complement(7..1110)
                     /locus_tag="VLC50_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013884360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HSF25965.1"
                     /translation="MTESSVEAAAISVVPYLTALADLGGSDLHCKVGSPPRVRIDGRL
                     RKLQAPTLTAEDTEAMAAEVMRGDLIDTFARSHEADFAFAVPGVGRFRVNAFRSRGSV
                     GLVFRRVAVGAIPLDDLGVPPVLATLAMEPRGLVLVTGPTGSGKTTTLAGMIDHINGN
                     REVHIVTIEDPIEVLHKDNLSMINQREVRVDTDDFAVAMRAAMRQDPDVILVGEMRDH
                     ETVKAALAAAETGHFVMSTLHTVDAQETVNRVIDFFPPHEQKQVRLSLAGALRGIVCQ
                     RLVPRADGLGRCVAMEVCINTGRIADAISDPDKTATISDLVKEGSFYGMQTFDQHLVA
                     LIRDGVVTLEAAMLAASNPHDLTVELRRLGLVA"
     gene            complement(1180..1401)
                     /locus_tag="VLC50_00460"
     CDS             complement(1180..1401)
                     /locus_tag="VLC50_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013872453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding carrier protein"
                     /protein_id="HSF25966.1"
                     /translation="MTEEVRAEMVANVMTVVVGVGDRIDGGDTLVLLESMKMEIPVLA
                     EGPGTVRAVHVAEGDVVQEGDVLVVLDRR"
     gene            1522..2082
                     /locus_tag="VLC50_00465"
     CDS             1522..2082
                     /locus_tag="VLC50_00465"
                     /inference="COORDINATES: protein motif:HMM:NF036410.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5317 family protein"
                     /protein_id="HSF25967.1"
                     /translation="MALVLLILVLATGLGLALGGHPRGFARLRVRLVGLVALAALVQL
                     LGLALASDRAPLLWAVLAITGLLTGAFVVANVAVPGVGLIGTGLVLNLLVVAANGGMP
                     VLARSVLLAGVDPGRALADPVHVPVEGARLAWLGDVVPVLNPLHREVVSIGDLLVAAG
                     IGLLVVTGMRRGRRAAPPPVSPSPAR"
     gene            complement(2091..3365)
                     /locus_tag="VLC50_00470"
     CDS             complement(2091..3365)
                     /locus_tag="VLC50_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017592714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HSF25968.1"
                     /translation="MSLVRGDGPSRLTGSGVVIVLTGIIVMLSVTLTAADGLALPYVS
                     LAFAALVCAGELVRVTLPGNRDAAPIATAGALGYALLPSFSDRVADYPAAQVVAVVAV
                     AMAMGALPHVAAGRAPALDALCRRLLAIGAAAVAFRSTPLRRTTGDLDPWQQAVVLIV
                     VVLLALVLDAVLAAVVRAGQNGAPFEAVLRDELRAVGGIGASIGSTGVLIALAAQFMG
                     YWALPVFAVPLLLTQFAFRRFATVRATYLQTIRSLARVTEVGGYTETGHARRVSQLAV
                     AVGRDLGMSEPELLELEYAALMHDIGQLSLADPIPGGATVVASPEQQRQIAEYGASVI
                     RQTGVLDSVATIVELQAEPYRRPHEDPRGGIPLASRIIRAVNAYDDLVGDAFEMSRRL
                     ESLERLRLGMAYDYDPRVVDSLARIVDRSARY"
     gene            complement(3362..>4011)
                     /locus_tag="VLC50_00475"
     CDS             complement(3362..>4011)
                     /locus_tag="VLC50_00475"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017539919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HSF25969.1"
                     /translation="AVETKDLYTRGHSERVATASVMIARQIGMREDRVSVLRYAGILH
                     DVGKLGVPTRVLQKSGRLTDEEYEAIKLHPVRGLEVVRQIEFLGEAYAGIMHHHERID
                     GRGYPMGLSGEQIPEFARVIAVADAFDSMTTTRSYRDARPIDEAIAELHRCSGTQFDP
                     VMVEAMVRALEAEPWAPEVPPLVPVDDEQTQGTGYDHDDPTVTIGPGAPDGGSGR"
BASE COUNT          556 a         1440 c         1456 g          559 t
ORIGIN      
        1 gaccggtcag gcgaccaggc cgagccggcg cagctccacg gtcaggtcgt gcgggttgga
       61 cgcggcgagc atcgcggcct cgagcgtcac cacgccgtcg cggatcagcg cgaccaggtg
      121 ctggtcgaag gtctgcatcc catagaacga gccctccttg accaggtcgg agatcgtcgc
      181 ggtcttgtcg gggtcggaga tcgcgtcggc gatgcggccg gtgttgatgc agacctccat
      241 cgccacgcag cgacccagcc cgtcggcgcg cggcacgagc cgctggcaga cgatgccgcg
      301 cagcgcgccg gcgagcgaga gccggacctg cttctgctcg tgcggcggga agaagtcgat
      361 gacccggttg acggtctcct gcgcgtcgac ggtgtgcagc gtggacatga cgaagtgccc
      421 ggtctcggcc gcggccaggg ccgccttgac ggtctcgtgg tcacgcatct cgccgacgag
      481 gatgacgtcg gggtcctggc gcatcgcggc gcgcatggcc accgcgaagt cgtcggtgtc
      541 gacccgcacc tcgcgctggt tgatcatcga gaggttgtcc ttgtgcaaca cctcgatcgg
      601 gtcctcgatc gtgacgatgt gcacctcgcg gttgccgttg atgtggtcga tcatgccggc
      661 cagcgtggtg gtcttgcccg acccggtcgg cccggtaacc agcacgagcc cgcgcggttc
      721 catcgccagg gtggccagca ccggcggcac gccgaggtca tcgagcggga tcgcgccgac
      781 ggcgacccgg cggaagacca gcccgaccga gccgcgcgag cggaacgcgt tgacgcggaa
      841 ccggcccacg ccgggtacgg cgaacgcgaa gtcggcctcg tggctgcggg cgaacgtgtc
      901 gatcaggtcg ccgcgcatca cctcggcggc catcgcctcg gtgtcctcgg cggtcagcgt
      961 cggcgcctgc agcttgcgca gccgcccatc gatgcgcacc cgcggcggtg agccgacctt
     1021 gcagtgcagg tcggagcccc ccaggtcggc cagggccgtc aggtagggga cgacggagat
     1081 cgcagcagcc tcgacagacg attcagtcac gtcgtgtgtt tcgggagccc ggggcggttc
     1141 cttgagtccg caacggtccc tgtcggtgac gcggcccagt cagcggcgat cgaggacgac
     1201 cagcacgtcg ccctcctgaa ccacgtcgcc ctcggccacg tgcaccgccc gcaccgtgcc
     1261 cggcccctcg gccagcacgg ggatctccat cttcatcgac tccagcagca cgagggtgtc
     1321 accaccgtcg atgcggtcgc ccacgcctac gaccaccgtc atgacgttgg ccaccatctc
     1381 ggcgcggacc tcctcggtca cccgccacct cctcgcgctg gaccgcctga cggccaggcc
     1441 ccgaccaccg gggcgtcagc gggaacctac cggtgcgcgt gccggcgagg cacgacggcg
     1501 caccgcgggc agactgtcgc cgtggccctg gtcctgctca tcctcgtcct ggccaccggt
     1561 ctgggcctgg ccctgggtgg gcatccgcgc ggcttcgccc ggctgcgggt ccggctcgtc
     1621 gggctggtgg cgctggcggc cctcgtccag ctgctcggcc tcgcactggc ctccgaccgt
     1681 gcgccgctgc tctgggcggt gctggcgatc accgggctgc tcaccggcgc gttcgtggtc
     1741 gccaacgtgg ccgtgcccgg cgtcgggctc atcggcaccg ggctggtgct caacctgctg
     1801 gtcgtcgccg ccaacggcgg gatgccggtg ctggccaggt ccgtcctcct ggccggggtc
     1861 gaccccggcc gcgcgctcgc cgaccccgtc cacgtgccgg tggaaggagc ccggctggcc
     1921 tggctcggcg acgtggtgcc ggtgctcaac ccgctgcacc gggaggtggt cagcatcggc
     1981 gacctgttgg tcgctgccgg gatcggcctg ctggtcgtga ccgggatgcg ccgcggccga
     2041 cgggctgcgc cccctccggt gtcccccagc ccggcgcggt aggggtcggc tcagtagcgg
     2101 gcggagcggt cgacgattcg ggccagcgag tcgaccaccc gagggtcgta gtcataggcc
     2161 atgcccaggc gcagccgttc gagcgactcg agccgccggc tcatctcgaa cgcgtcaccg
     2221 acgaggtcgt cgtacgcgtt gaccgcccgg atgatccgac tggccaacgg gatgcctccg
     2281 cgggggtcct cgtggggacg gcggtagggc tcggcctgca gctcgacgat ggtggccacc
     2341 gagtcgagca cgccggtctg gcggatcacc gaggcgccgt actcggcgat ctggcgctgc
     2401 tgctccggcg aggccaccac cgtggcgccg cccgggatcg ggtcggcgag agagagctgg
     2461 ccgatgtcgt gcatgagcgc ggcgtactcg agctcgagca gctccggctc gctcatcccc
     2521 aggtcgcgtc cgaccgccac cgcgagctgg ctgacccgcc gggcatggcc ggtctcggtg
     2581 tagccgccga cctcggtgac ccgggccagc gagcggatcg tctgcaggta ggtggcccgc
     2641 accgtggcga agcgacggaa cgcgaactgg gtgagcagca gcggtacggc gaagaccggc
     2701 agcgcccagt agcccatgaa ctgcgcggcc agcgcgatga gcacgccggt cgacccgatc
     2761 gaggcgccga tgccgccgac cgcgcgcagc tcgtcacgca ggacggcctc aaacggcgcg
     2821 ccgttctggc cggcgcgcac cacggcagcg agcacggcat cgagcaccag ggcgagcagg
     2881 acgacgacga tgaggacgac ggcctgctgc cacgggtcca gatcacccgt cgtacgacgc
     2941 agcggggtgg accggaacgc gacggcggcc gccccgatgg cgagcagccg ccggcacagc
     3001 gcatcgaggg caggggcccg gcccgccgcg acgtgcggca gcgcgcccat cgccatcgcg
     3061 accgcgacaa cggcgaccac ctgggcggcg gggtagtcgg caacccggtc ggagaagctt
     3121 ggcagcagcg cgtagcccag cgcgccggcg gtggcgatcg gtgcagcgtc gcggttgccg
     3181 ggcagcgtca cccggacgag ctcgccggcg cagacgaggg cggcgaaggc cagcgagacg
     3241 tagggcaggg cgagcccgtc ggcggcggtc agggtgaccg acagcatcac gatgatgccg
     3301 gtgagcacga tgaccacgcc cgagccggtg agtcggctcg ggccgtcccc cctcaccagg
     3361 ctcaccgccc cgacccgccg tccggggcgc ccggcccgat ggtcaccgtc gggtcgtcgt
     3421 ggtcatagcc ggtgccctgg gtctgctcgt cgtcgaccgg cacgagcggt ggcacctcgg
     3481 gcgcccaggg ctcggcctcg agcgcgcgca ccatcgcctc caccatcacc gggtcgaact
     3541 gggtgccgga acagcggtgc agctcggcga tcgcctcgtc gatcgggcgg gcgtcccggt
     3601 aggagcgagt ggtggtcatc gagtcgaacg cgtcggccac ggcgatgacc cgggcgaact
     3661 ccgggatctg ctcaccagac aggcccatcg gatagccgcg accgtcgatg cgctcgtgat
     3721 ggtgcatgat cccggcgtag gcctcgccca ggaactcgat ctgacggacc acctccaggc
     3781 cgcgcaccgg gtgcagcttg atcgcctcgt actcctcgtc ggtgagccgg cccgacttct
     3841 gcagcacccg ggtcggaacc cccagcttgc cgacgtcgtg gaggatcccc gcgtagcgca
     3901 gcacgctgac ccggtcctcg cgcatcccta tctgacgggc gatcatcact gaggcggtgg
     3961 ccacccgctc gctgtggccg cgggtgtaga gatccttggt ctcgacggcc t
//
LOCUS       DATIBB010000014         3717 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_1453158, whole genome shotgun sequence.
ACCESSION   DATIBB010000014 DATIBB010000000
VERSION     DATIBB010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3717)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3717)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3717
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1453158"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..541
                     /locus_tag="VLC50_00480"
     CDS             <1..541
                     /locus_tag="VLC50_00480"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HSF25970.1"
                     /translation="IAKERAERARRAEQARRAAAAARVAAAAAAARTAGGGSSTGGAS
                     AASTTASSGTSYGTVAGAAAAISYAREQIGKPYVWAAAGPDTFDCSGLTMRAWQAGGV
                     SLPHYSVAQWDRVKTVDLDDLRAGDLVFFATSKTNPATIYHVGLYIGGGQMIEAPYTG
                     ENVRISSIYRASLFGAGRP"
     gene            602..1765
                     /locus_tag="VLC50_00485"
     CDS             602..1765
                     /locus_tag="VLC50_00485"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HSF25971.1"
                     /translation="MRCLRVLLVAALVIGGGAAPAVAATEPTPPPPGTATSPQDVERS
                     TRVLVASLDARLADLGERVEALRADVARAVEAYDGARVRLAAADQAAEAASARLHQAQ
                     RRVRDAEDALAAYAAAVYRSGGPLAPVGTLFDSGGVQSFANRLDLLDRVGDAEAKIIK
                     ELLDAQAAGAAARDAARAAVRTAQAALAQVTAARDAATERLAQAEATLADFDRQREDL
                     LAALAAARESTIEAEAARAQARALAAARAGTPFALIDLAERDGAVTPAAARAIAFAQA
                     QLGKPYEWAADGPQTFDCSGLTMRAWQAGGVSLPHYSVAQWQRSRPVDLADIRPGDLV
                     FFADDTSNPATIYHVGLYVGRGLMIEAPYTGAVVRVSPIWRPSLLGAARPTPG"
     gene            complement(1762..2256)
                     /locus_tag="VLC50_00490"
     CDS             complement(1762..2256)
                     /locus_tag="VLC50_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018514302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HSF25972.1"
                     /translation="MDFDVTIEIPKGQRNKYEVDHESGRIRLDRMLFTSTRYPADYGF
                     IEDSLGQDGDPLDALVLLDEPTFPGCLIRCRAIGMYRMRDEAGRDDKVLCVPATDPRS
                     EHLRDIFHVPEFDKLEIQHFFTVYKDLEPGKSVEGAEWVGRAEAEAEVIASYERAKAA
                     AAEH"
     gene            2300..>3717
                     /gene="dacB"
                     /locus_tag="VLC50_00495"
     CDS             2300..>3717
                     /gene="dacB"
                     /locus_tag="VLC50_00495"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HSF25973.1"
                     /translation="MGRSHRGGVAALVVVLLVLGAAAAMLTGQVPSPLDRRPATAAGP
                     TAAGSTTPGTGPSSAPVAGLLQPSPAAPAPVLAGLPRLVPPPTTAGLQAALGSLLGTA
                     SLGPDVGAVVLDGASGAVLLDQGGSVAHTPASTLKLLTGAAALQVIGPQTRLTTSVVR
                     GAAPGELVLVGGGDPLLTAGETTADETASTPASLRELARRTAAAMRAAGVHAARVSVD
                     DTLFAPPAIAPTWEKHYVPSGVVAPVSALMVDQARTTPASSPGAPTGARHADPALAAG
                     QQLATLLAAEGITVSAPVARATAAAGAQPLATVESPAVGEIVERMLTTSDNDIAEALL
                     RLVAVRSGAPATFDGGTAATIAALQQLGVPTQGVRLSDGSGLARLDAVPARTLAAVLV
                     AAASPEHPALRPLLSGLPVAGFNGTLAGRFTDGGARGIVRAKTGSLTGVSTLAGTLTD
                     ADGSLLVFAVLADEVPQTGTLAA"
BASE COUNT          429 a         1424 c         1382 g          482 t
ORIGIN      
        1 gatcgccaag gaacgggccg agcgcgcccg tcgcgccgag caggcccggc gcgcggcggc
       61 ggcggcccgg gtggctgcgg cagcggcggc ggcccggacg gccggcggcg gctcgagcac
      121 cggcggcgcg tcggccgcct cgacgaccgc gtcgagcggc acgtcctacg gcaccgtggc
      181 cggggccgcg gcggcgatct cctatgcccg cgagcagatc ggcaagccct atgtgtgggc
      241 ggcggccggc ccggacacct tcgactgctc ggggctgacc atgcgggcct ggcaggccgg
      301 cggggtgtcg ctgccgcact acagcgtggc ccagtgggac cgggtcaaga cggtcgacct
      361 ggacgacctg cgggccggtg acctggtgtt cttcgccacc agcaagacca acccggcgac
      421 gatctaccac gtggggctct acatcggcgg cggccagatg atcgaggccc cctataccgg
      481 ggagaacgtg cgcatctcga gcatctatcg agccagcctg ttcggcgccg gccggccctg
      541 attcgcgccg cttcgatcaa tctcgtcgcg ggcctgcggg ggcgggcgca tcctggcagc
      601 ggtgcgatgc ctgcgagtgc ttctcgtcgc ggccctggtg atcgggggtg gagccgcccc
      661 ggccgtggcc gccaccgagc cgaccccgcc gccgcctggc accgcgacca gcccgcagga
      721 cgtcgagcgc tcgacccggg tgctcgtcgc atcgctggac gcccggctgg ccgacctggg
      781 cgagcgggtc gaggcgctgc gcgccgatgt cgcccgcgcg gtggaggcgt acgacggtgc
      841 ccgggtgcgg ctggccgccg ccgaccaggc agccgaggcc gcctccgccc ggctccacca
      901 ggcgcagagg cgcgtgcgcg acgccgagga cgccctcgcc gcctacgccg ccgccgtcta
      961 ccgctccggt gggccgctgg ccccggtcgg cacgctgttc gactcgggcg gcgtgcagag
     1021 cttcgccaac cggctggacc tgctcgaccg ggtcggcgac gccgaagcca agatcatcaa
     1081 agagctgctg gacgcgcagg cggccggggc ggccgcgcgc gacgccgccc gtgccgctgt
     1141 ccgcacggcg caggccgcgc tcgcccaggt cacggcggct cgagacgccg ccaccgagcg
     1201 gctggcccag gccgaggcga cgctcgccga cttcgaccgt cagcgggagg acctgctcgc
     1261 cgcgctggcc gccgcgcgcg agagcaccat cgaggccgag gccgcccgcg cgcaggcccg
     1321 ggcgctcgca gcggcgcggg caggcacgcc gttcgcgctg atcgacctgg ccgagcggga
     1381 cggcgcggtc acgccggccg ccgcgcgggc catcgccttc gcacaggccc agctgggcaa
     1441 gccctacgag tgggccgccg acggcccgca gacgttcgac tgctccgggc tgacgatgcg
     1501 agcctggcag gccggcgggg tgtcgctgcc gcactacagc gtcgcgcagt ggcagcgcag
     1561 ccgccccgtc gatctcgccg acatccgccc gggcgatctc gtcttcttcg ccgacgacac
     1621 gagcaacccg gcgacgatct accacgtggg cctctacgtc ggacgtgggc tgatgatcga
     1681 ggccccctat accggtgcgg tcgtgcgcgt ctcgccgatc tggcgtccca gcctgctggg
     1741 cgccgctcgc cctacgcccg gctagtgctc cgcggcggcg gccttggcgc gctcgtagga
     1801 ggcgatcacc tcggcctcgg cctcagcccg gcccacccac tcggcgccct cgacgctctt
     1861 gcccggctcg aggtccttgt agacggtgaa gaagtgctgg atctcgagct tgtcgaactc
     1921 cggcacgtgg aagatgtcgc gcaggtgctc gctgcgtggg tcggtcgccg gcacgcacag
     1981 caccttgtcg tcgcgcccgg cctcgtcacg catccggtac atgccgatgg cccggcaccg
     2041 gatcaggcag cccgggaacg tcggctcgtc cagcagcacg agtgcgtcga gcgggtcgcc
     2101 gtcctggccc aggctgtcct cgatgaaccc gtagtcggcc gggtagcggg tcgaggtgaa
     2161 cagcatccgg tcgagccgga tccggccgct ctcgtggtcg acctcgtact tgttgcgctg
     2221 acccttgggg atctcgatgg tgacgtcgaa gtccacggct cctccaaggt cagatgacgg
     2281 ctcggttagt gtcccgcacg tgggcaggtc gcacaggggt ggtgtcgctg ccctcgtcgt
     2341 ggtgctgctg gtgctcggcg ccgccgccgc gatgctcacc ggtcaggtgc cctccccgct
     2401 cgaccgccgc ccggccacgg cagccggccc gacggcagcc ggctcgacca cacccggcac
     2461 cggcccctcc agcgctcccg ttgccgggtt gctccagccc tcgccggccg cgccggcgcc
     2521 ggtgctggcc ggcctgcccc ggctggtgcc gccgccgacc acggccggcc tgcaggcggc
     2581 gctgggctcg ctgctcggca ccgcgtcgct cggtcccgac gtcggtgccg tcgtgctcga
     2641 cggggccagc ggggcggtgc tgctcgacca gggcggcagc gtggcgcaca cccccgcatc
     2701 gaccctcaag ctgctcaccg gcgctgcggc gctgcaggtg atcgggccgc agacccggct
     2761 gaccacctcc gtcgtgcgtg gtgccgcccc gggggagctc gtgctggtcg gcgggggcga
     2821 tccgctcctc acggcaggtg agacgaccgc tgacgagacc gcgtcgaccc cggccagcct
     2881 gcgcgagctg gcccggcgca ccgccgcggc gatgcgcgcg gccggcgtcc acgccgcgcg
     2941 ggtgagcgtc gatgacacgt tgttcgcccc gccggcgatc gcgccgacct gggagaagca
     3001 ctacgtgccg tcgggagtgg tggccccggt ctcggcgctg atggttgacc aggcccggac
     3061 cacgccggcg tcctcgcccg gagcccccac cggggcccgg cacgccgatc cggcgctcgc
     3121 cgccggccag cagctcgcca ccctgctcgc cgccgagggg atcacggtca gcgcgccggt
     3181 cgcccgggcg actgccgccg ccggtgccca gccgctggcc accgtggagt caccggcggt
     3241 cggcgagatc gtcgagcgca tgctcacgac cagcgacaac gacatcgccg aggcgctgct
     3301 gcggctggtc gcggtgcgct cgggagcgcc ggcgacgttc gacggcggca cggcggcgac
     3361 catcgccgcc ctgcagcagc tcggggtgcc gactcagggc gtgcggctgt ccgacgggag
     3421 cgggctggcc cgcctcgacg ccgtacccgc tcgcaccttg gccgccgtcc tggtcgcggc
     3481 cgcctcgccc gagcacccgg cgctgcggcc gctgctgagc gggctgccgg tggccgggtt
     3541 caacgggacg ctggccggcc ggttcaccga cggtggcgcc cgcggcatcg tgcgggccaa
     3601 gaccggcagc ctgaccgggg tgagcacgct ggccggcacg ctcaccgatg ctgacggctc
     3661 gctgctcgtg ttcgcggtgc tggccgacga ggtgccgcag accggcacgc tggccgc
//
LOCUS       DATIBB010000015         3398 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_927777, whole genome shotgun sequence.
ACCESSION   DATIBB010000015 DATIBB010000000
VERSION     DATIBB010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3398)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3398)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3398
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_927777"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..242)
                     /locus_tag="VLC50_00500"
     CDS             complement(<1..242)
                     /locus_tag="VLC50_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020557142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HSF25974.1"
                     /translation="MHLAHLSLADFRSYPSVELPLHPGVTAFVGPNGQGKTNLVEAVG
                     YLATLGSHRVAQDAPLVRLGAERAVVRGAVVRDGRTT"
     gene            complement(255..1127)
                     /gene="gnd"
                     /locus_tag="VLC50_00505"
     CDS             complement(255..1127)
                     /gene="gnd"
                     /locus_tag="VLC50_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014143850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HSF25975.1"
                     /translation="MQIGLVGLGRMGANMRERLRAGGHTVVGYARTRAVSDVGSLEEL
                     VEALPAPRVVWVMVPAGEPTRTTIGVLEGLLSPGDVVVDGGNSRWTDDLVHAAQLAEH
                     GIGYVDCGVSGGVWGLQNGYALMCGGSVDDVAKVQPAFDTLKPPGEHGFVHAGPVGAG
                     HFAKMVHNGIEYAMMQAFAEGYELLAAADVVTDVPAVFRSWREGTVIRSWLLDLLDNA
                     LTDDPTLASLRGYADDSGEGRWTVQAAIDHAVPMPAISAALFARFASRQDDSPAMKAI
                     AAMRHQFGGHAVHQ"
     gene            complement(1154..2284)
                     /gene="dnaN"
                     /locus_tag="VLC50_00510"
     CDS             complement(1154..2284)
                     /gene="dnaN"
                     /locus_tag="VLC50_00510"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016437045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HSF25976.1"
                     /translation="MKLRVERDVLAEAVAWAARTLPQRPPVPVLAGLLLEAEGDVVRM
                     AGFDYEVSTRIEIPAEVDEPGRVLVSGRLLADICRSLPARPVELVTHEAKVVVTCGSA
                     RFTLLTLPVDEYPALPEMPAAAGIVAGDLFAHAVSQVSVAAGRDDTLPALTGIRVEIE
                     GESVTLAATDRYRLAVRQLRWSPEQSGSSAVALVPARSLTDTARALSAADKVTIALAT
                     TPGGEGLIGFEGNGRRTTQRLLDSEFPKYQTLWPREAATVAVVEAAPFIEAVKRVALV
                     AERNTPIRLSFTDGVVTLEAGAGEDAQASESLDVSVTGDDITIAFNPTYLLDGLGALD
                     DSHVRLSFTVASKPAVLSGTTGPDGDVDDSYRYLLMPVRVAG"
     gene            complement(2658..>3398)
                     /gene="dnaA"
                     /locus_tag="VLC50_00515"
     CDS             complement(2658..>3398)
                     /gene="dnaA"
                     /locus_tag="VLC50_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017624928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HSF25977.1"
                     /translation="YREVDILLVDDIQFLENKEQTQEEFFHTFNTLHNANKQIVISSD
                     RPPQQLITLEDRLRNRFQWGLTTDITPPELETRIAILRKKAAQEGLAAPPEVLEFIAS
                     KIATNIRELEGALIRVTAFASLNRQTVDLSLAEIVLRDLIPDNAGPEITAATIMAQTA
                     AYFGLSMEDLCGSSRSRVLVTARQIAMYLCRELTEMSLPKIGQQFGGRDHTTVMHADR
                     KIRQLMAERRSVYNQVTELTNRIKNQSR"
BASE COUNT          479 a         1199 c         1218 g          502 t
ORIGIN      
        1 gtcgtacgcc catcgcgtac gacggcgccg cgcaccaccg cccgctcggc gccgagccgc
       61 acgagcgggg catcctgcgc cacccggtgc gagccgagcg tggcgagata gccgaccgcc
      121 tcgacgaggt tggtcttgcc ctgcccgtta ggcccgacga acgcggtgac cccggggtgc
      181 agcggcagct cgacgctggg gtaggagcgg aagtcagcca gcgacaggtg ggcgagatgc
      241 accggcgcgc acgctcactg atgcaccgcg tgcccaccga actggtgacg catcgcggcg
      301 atcgccttca tggccgggga gtcgtcctgg cgggaggcga accgggcaaa cagcgcggcc
      361 gagatcgccg gcatcggcac cgcgtggtcg atcgcggcct gcacggtcca gcgaccctcg
      421 ccggagtcgt cggcgtagcc gcgcagcgag gccagcgtcg ggtcgtcggt cagcgcgttg
      481 tcgagcaggt cgagcagcca ggaccggatc acggtgccct cgcgccagga ccggaacacc
      541 gccggcacgt ccgtgacgac gtcggcggcg gccagcagct cgtagccctc ggcaaaggcc
      601 tgcatcatgg cgtactcgat gccgttgtgg accatcttgg cgaagtgccc ggcgccgacc
      661 gggccggcgt gcacgaagcc gtgctcgccg ggcggcttga gcgtgtcgaa ggccggctgc
      721 accttggcca cgtcgtcgac ggacccgccg cacatcagcg cgtagccgtt ctgcaggccc
      781 cagaccccgc cggagacccc gcagtcgacg tagccgatgc cgtgctcggc cagctgggcc
      841 gcgtgcacca ggtcgtcggt ccagcgggag ttgccgccat cgacgaccac gtcgccgggc
      901 gagagcagcc cctcgagcac gccgatcgtc gtgcgcgtgg gctcaccggc cgggaccatc
      961 acccagacca cccggggtgc gggcagcgct tcgaccagct cctcgagcga gccgacgtcg
     1021 gagaccgccc gggtgcgcgc gtagccgacg acggtgtgcc cgccggcgcg cagccgctcg
     1081 cgcatgttgg cacccatgcg cccgagcccg accagtccga tctgcacgtg tgcctccttg
     1141 cgcccggtgg ccgtcagccc gcgacccgca ccggcatgag caggtaacgg tagctgtcgt
     1201 cgacgtcgcc gtcggggccg gtggtgccgc tgagcacggc cggcttggac gccaccgtga
     1261 acgagagccg cacgtgcgag tcgtcgaggg ccccgagccc gtcgagcagg taggtcgggt
     1321 tgaacgcgat cgtgatgtcg tcaccggtca ccgacacgtc aagcgactcg gaggcttgag
     1381 cgtcctcgcc ggcgccggcc tccagcgtga ccaccccgtc ggtgaacgag agccggatcg
     1441 gggtgttgcg ctcggccacc agcgcgaccc gcttgaccgc ctcgatgaac ggggccgcct
     1501 cgaccaccgc cacggtggcc gcctcgcgcg gccacagcgt ctggtacttg gggaactcgc
     1561 tgtcgagcag ccgctgcgtc gtacgacgtc cgttgccctc gaagccgatc agcccctcgc
     1621 cgccgggggt ggtggccagc gcgatggtca ccttgtcggc cgccgacagc gcgcgggcgg
     1681 tgtcggtgag gctgcgcgcc ggcaccagcg cgacggccga ggagccgctc tgctcgggcg
     1741 accagcgcag ctggcgcacc gccagccggt agcggtcggt cgccgccagc gtcaccgact
     1801 cgccctcgat ctcgacccgg atgccggtca gcgccggcag cgtgtcgtca cgcccggccg
     1861 cgaccgagac ctgggacacc gcgtgggcga acaggtcgcc ggccacgatg ccggccgcgg
     1921 cgggcatctc gggcagcgcc gggtattcgt cgaccggcag cgtcagcagc gtgaaccggg
     1981 cgctgccaca ggtgacgacc accttggcct cgtgggtgac cagctcgact gggcgagccg
     2041 gcaggctgcg gcagatgtcg gccagcagcc ggccggacac cagcacccgt cccggctcgt
     2101 cgacctcggc ggggatctcg atccgcgtcg agacctcgta gtcgaagccg gccatccgca
     2161 cgacgtcgcc ctcggcctcc agcagcaacc ccgccagcac cggcaccggg ggtcgctgcg
     2221 gcagcgtgcg tgcggcccag gccaccgcct ctgccagaac gtcgcgttcg acccgaagct
     2281 tcaccggtca ccgcctcctg ctcgtcgtag gcgccgaagt gtgccacccc ggccattggt
     2341 acgccgaacc ccggccggac gccaactgtc cccaggtcgg cgcgccctcg tcgttgggtc
     2401 tatccatcga tgtctagaga acagacgttg tcgtcggtgc cgtggacact ggggaaaact
     2461 cgcgttgccg caggtcagcg cgtcgatgcc ggcggggaca gcctggggat gaccgttcgc
     2521 ggcgtctcgg cccggctggg gacgggcgcg tcggccgctc gcgtcgtacg cagtccgtcc
     2581 accgcgaccg tggtgatctc cacagagttg tccacaggct gtgtgccacg acgtccggat
     2641 catgagacgg cgcgccctca acgggactgg ttcttgatcc ggttggtcag ctcggtgacc
     2701 tggttgtaga cgctgcgccg ctcggccatc agctggcgga tcttgcggtc ggcgtgcatg
     2761 accgtggtgt ggtcgcggcc gccgaactgc tggccgatct tgggcagcga catctcggtg
     2821 agctcgcggc acaggtacat cgcgatctgg cgggcggtga ccagcacccg gctgcgcgag
     2881 gagccgcaca ggtcttccat cgacagcccg aagtaggccg cggtctgcgc catgatcgtc
     2941 gccgcggtga tctcggggcc ggcgttgtcg gggatcaggt cgcgcagcac gatctcggcc
     3001 agcgacaggt cgacggtctg gcggttgagg ctggcgaagg cggtcacccg gatcagcgcg
     3061 ccctcgagct cgcggatgtt ggtcgcgatc ttgctggcga tgaactcgag cacctcgggc
     3121 ggggccgcca gtccctcctg ggccgccttc ttgcgcagga tcgcgatgcg cgtctcgagc
     3181 tcgggcgggg tgatgtcggt ggtcaggccc cactggaacc ggttgcgcag ccggtcctcc
     3241 agcgtgatca gctgctgcgg cggccggtcg ctggagatga cgatctgctt gttggcgttg
     3301 tgcagcgtgt tgaaggtgtg gaagaactcc tcctgcgtct gctccttgtt ctccaagaac
     3361 tggatgtcgt cgacgagcag gatgtcgacc tcgcggta
//
LOCUS       DATIBB010000016         3750 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_533681, whole genome shotgun sequence.
ACCESSION   DATIBB010000016 DATIBB010000000
VERSION     DATIBB010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3750
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_533681"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..255
                     /locus_tag="VLC50_00520"
     CDS             <1..255
                     /locus_tag="VLC50_00520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF25978.1"
                     /translation="GYDVLRRHPVVLARFAARHVAAQVEANHAGLSAVRTDLRDVVTP
                     DVVEAAAVVLDREGARLLGLTRAVDLVEQALRGQRFRARL"
     gene            330..2273
                     /gene="dxs"
                     /locus_tag="VLC50_00525"
     CDS             330..2273
                     /gene="dxs"
                     /locus_tag="VLC50_00525"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014145383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HSF25979.1"
                     /translation="MGLLETIQGPRDLRRMTPDELRTLAGEIRSFLVANVARTGGHLG
                     PNLGAVELTLAVHRVFDSPHDRILFDTGHQAYVHKIVTGRAGRFPSLRQAGGLSGYPC
                     RAESEHDVIENSHASTALSWADGIAKALQLHGQADRTVVAVVGDGALTGGMAWEALNN
                     IASGKDRSLVIVVNDNERSYAPTIGGLATHLATLRTTRGYERFLQWGKQTLARTPVVG
                     PPIYDTLHGMKKGLKDIVAPQGMFEDLGLKYVGPVDGHDVEALERALRRAKSYGGPVI
                     VHAITHKGLGYPPAENDQADQFHSVGVIDPETGESLSTATQTWTGVFADELVTLGAER
                     PDLVGVTAAMLIPVGLARFAERFPGRVFDVGIAEQHATTSAAGLALEGLHPVVAVYST
                     FLNRAFDQVLMDVALHRAGVTFVLDRSGVTGDDGPSHNGMWDLAVLQVVPGLRLAAPR
                     DEPSLREELREAVAVSDAPTVVRFAKGALPEPVPALERVDGVDVLHRGTTPDVLLVSV
                     GSMARTCLDVAARAATQGVGVTVVDPRWVKPVPEVLVRMAARHRLVVSVEDGVRAGGV
                     GSALAQALRDADLDVPLRDFGIPARFLDHGKRAAILAELGLTGQEISRRVVELIARLD
                     GRSVLDDENPRPQLDTAARTAVE"
     regulatory      2278..2385
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            2467..>3750
                     /locus_tag="VLC50_00530"
     CDS             2467..>3750
                     /locus_tag="VLC50_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018776127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase family
                     protein"
                     /protein_id="HSF25980.1"
                     /translation="MSDRSWGFATRAVHAGGAPDPTTGARAVPIYMSSGFVFEDTDDA
                     ANLFALQKYGTVYSRISNPTVAAFEERMASLEGGLGAVAASSGQAAAFLTFAALTGAG
                     DHIVASASLYGGTINQLSVTLGRFGVETSFVRGTDPADYAAAITAKTKLIFVELLSNP
                     SCEVADLAGLAEVAHAAGIPLIVDATTASPYLCRPIEHGADIVVHSATKFIGGHGTTM
                     GGVVVDSGTFDWGNGNFPTMTEPAQSYGGIRWWDNFGEYGFLTKLRSEQLRDIGACLG
                     PMSAFMLLQGLETLPQRMRAHVANAQRVAEWLQADERVAWVRYAGLPGHPHADRVARY
                     VPEGPGSVFSFGVVGGRAAGQRFIESVELCSHLANIGDAKTLVIHPASTTHQQLSDEQ
                     LEAGGVPPDLVRISVGIEDAEDILWDLDQALTEATK"
BASE COUNT          546 a         1381 c         1310 g          513 t
ORIGIN      
        1 ggctacgacg tgctgcggcg ccacccggtc gtgctggccc ggttcgccgc ccgacacgtc
       61 gccgcgcagg tggaggccaa ccatgcgggc ctcagcgcgg tgcgcaccga cctgcgcgac
      121 gtcgtcaccc ccgatgtcgt ggaggcggct gccgtcgtac tcgatcggga gggggcccgg
      181 ctgctcggcc tgaccagggc cgtcgacctg gtcgagcagg cgctgcgcgg ccagcgattt
      241 cgcgcccggc tctgaggcct gaccacggcg ggcgcgacgg ctacctagac tgggcccgct
      301 atcggccgag cctggaggag gatcaccgga tggggctgct cgagaccatc caggggccac
      361 gcgacctgcg ccgcatgaca cccgacgagc tgcgcacact ggctggcgag atccggtcct
      421 tcctggtcgc caacgtggcc cgcaccggcg ggcaccttgg tcccaacctc ggagcggtcg
      481 agctgaccct ggccgtgcac cgggtcttcg actcgccgca cgaccggatc ctcttcgaca
      541 ccggccacca ggcctacgtg cacaagatcg tcaccgggcg ggccggtcgg ttccccagcc
      601 tgcgccaggc cggtgggctc tccggctacc cgtgccgggc cgagtccgag cacgacgtca
      661 tcgagaactc ccacgcctcc accgcgctct cgtgggccga cggcatcgcc aaggcgctgc
      721 agctgcacgg gcaggccgac cgcacggtcg tcgcggtcgt cggcgacggg gcgctcaccg
      781 gcgggatggc ctgggaggcg ctcaacaaca tcgccagcgg caaggaccgc tcgctggtga
      841 tcgtcgtcaa cgacaacgag cgctcctacg ccccgaccat cggcgggctg gccacccacc
      901 tggcgaccct gcgcaccacc cgcggctacg aacgcttcct ccagtggggc aagcagacgc
      961 tggcccgcac cccggtcgtc gggccgccga tctacgacac gctgcacggc atgaagaagg
     1021 ggctcaagga catcgtcgcg ccccagggga tgttcgagga cctcggtctc aagtacgtcg
     1081 ggccggtcga cgggcacgac gtcgaggcgc tcgagcgggc cctgcgccgg gccaagtcct
     1141 acggcggtcc ggtcatcgtc cacgcgatca cccacaaggg gctcggctac cccccggccg
     1201 agaacgacca ggccgaccag ttccacagcg tgggcgtcat cgacccggag accggcgagt
     1261 cgctgtccac cgccacgcag acctggaccg gtgtcttcgc cgacgagctg gtgacgctcg
     1321 gagccgagcg ccccgacctg gtcggggtca ccgccgcgat gctgatcccg gtcgggctgg
     1381 cccgcttcgc cgaacgcttt cccggacggg tcttcgacgt gggcatcgcc gagcagcacg
     1441 ccaccaccag cgctgccggg ctcgcgctgg agggcctgca ccccgtcgtc gcggtctact
     1501 ccacgttcct caaccgggcc ttcgaccagg tgctcatgga cgtcgcgctg caccgggccg
     1561 gagtcacgtt cgtgctcgac cgctccggcg tgaccggtga cgacgggccc agccacaacg
     1621 gcatgtggga cctcgccgtg ctccaggtcg tccccggcct gcgcctggcc gccccccgcg
     1681 acgagccctc gctgcgcgaa gagctgcgcg aggcggtcgc ggtgtccgac gcgccaaccg
     1741 tcgtccgctt cgccaagggg gcgctgcccg agcccgtgcc cgcgctcgag cgggtcgacg
     1801 gcgtcgacgt gctgcaccgg gggaccaccc ccgacgtgct gctggtcagc gtgggctcga
     1861 tggcgcgcac ctgcctcgac gtggccgccc gagccgccac ccagggcgtg ggggtgacgg
     1921 tcgtcgaccc gcggtgggtc aagccggtgc ccgaggtgct cgtccgcatg gccgcccggc
     1981 accggctcgt ggtcagcgtc gaagacggcg tgcgcgccgg gggagtgggg tcggcgctgg
     2041 cccaggcgct gcgcgacgcc gacctcgacg tgccgctgcg cgacttcggc atcccggccc
     2101 gcttcctcga ccacggcaag cgcgcggcca tcctggccga gctcggactg accgggcagg
     2161 agatctcccg acgggtcgtc gagctgatcg cgcgcctcga cgggcgctcc gtcttggatg
     2221 acgagaaccc tcgtccacaa cttgacaccg ccgcgcgcac cgcggtcgaa tgacgaccat
     2281 ccatccagag cgaccgagag acctggctcg atgacgtcgc agcaaccacc cctcggggcc
     2341 tggtgctaat gccagcaccg atgaggactg tgagcgcgat cgaccacatg aggtctcccc
     2401 tctggcggcg ctcatctggt gcgcggtcgt gaccgatccg ctagccgatg atgttggaag
     2461 gaagtcatga gcgaccgcag ctgggggttc gctacgcgcg ccgtgcacgc gggcggcgcg
     2521 cctgatccca cgaccggggc ccgtgcggtg ccgatctaca tgagcagcgg gttcgtcttc
     2581 gaggacaccg acgacgccgc caacctgttc gcgctgcaga agtacggcac ggtctacagc
     2641 cggatctcca acccgacggt ggccgccttc gaggagcgca tggccagcct cgagggtggg
     2701 ctcggcgcag tggcggccag cagcggccag gcggcggcgt tcctgacctt cgccgcactg
     2761 accggtgccg gcgaccacat cgtggccagc gccagcctct atggcggcac gatcaaccag
     2821 ctcagcgtca cgctggggcg cttcggcgtc gagaccagct tcgtgcgcgg caccgacccg
     2881 gccgactacg ccgccgcgat caccgccaag accaagctga tcttcgtgga gctgctgtcc
     2941 aacccgtcgt gcgaggtggc cgacctggcc gggctggccg aggtggccca cgccgcgggc
     3001 atcccgctga tcgtcgacgc caccaccgcg agcccctacc tgtgccggcc gatcgagcac
     3061 ggcgccgaca tcgtggtgca ctcggcgacg aagttcatcg gcgggcacgg cacgacgatg
     3121 ggcggggtcg tcgtcgactc cggcaccttc gactggggca acggcaactt cccgacgatg
     3181 accgagccgg cgcagtccta cggcggcatc cgctggtggg acaacttcgg tgaatacggc
     3241 ttcctcacca agctgcgcag cgagcagctg cgcgacatcg gggcctgcct ggggccgatg
     3301 agcgccttca tgctgctgca gggcctggag accctgccgc agcggatgcg cgcccacgtg
     3361 gccaacgccc agcgggtagc cgagtggctg caggccgacg agcgggtcgc ctgggtgcgc
     3421 tacgccgggc tgcccgggca cccgcacgca gaccgggtgg ctcgctacgt gcccgagggg
     3481 ccggggtcgg tgttctcctt cggagtggtc ggcgggcgtg ccgccggtca gcgcttcatc
     3541 gagtcggtcg agctgtgcag ccacctggcc aacatcggtg acgccaagac cctggtcatc
     3601 cacccggcca gcacgaccca ccagcagctc agcgacgagc agctcgaggc cggcggggtg
     3661 ccgcccgacc tggtgcggat cagcgtcggc atcgaggacg cggaggacat cctgtgggac
     3721 ctggaccagg cgctgaccga ggcgaccaag
//
LOCUS       DATIBB010000017         2566 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_1323391, whole genome shotgun sequence.
ACCESSION   DATIBB010000017 DATIBB010000000
VERSION     DATIBB010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2566
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1323391"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..635)
                     /locus_tag="VLC50_00535"
     CDS             complement(<1..635)
                     /locus_tag="VLC50_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015800944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HSF25981.1"
                     /translation="MSGHSKWATTKHKKAVVDAKRGKLFARLIKNIEVSARTGGGDPA
                     GNPTLYDAIQKAKKNSVPADNIDRAVKRGSGEDGSGADWQTITYEGYAAGGVALLIEC
                     LTDNRNRAASDVRVAVTRNGGSMADPGSVSYLFHRKGVVIVPKADGLTEDDVLLAVLE
                     AGAEEVNDLGEAFEVVSEAGDLVAVRSALQQAGLDYDSAEAQWVPSMEVTV"
     gene            complement(675..1289)
                     /gene="pdxT"
                     /locus_tag="VLC50_00540"
     CDS             complement(675..1289)
                     /gene="pdxT"
                     /locus_tag="VLC50_00540"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018908231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HSF25982.1"
                     /translation="MPSPSAPVIGVLALQGDVREHVAALAALGVASSAVRRPSELDTV
                     DALVIPGGESTTMHKLAAAFDLDEPLRKRLGEGMPAFGSCAGMIMLADRVLDAPPDQQ
                     TFGGIDMTVRRNAFGRQVDSFEGELEMAGVDGGPVHAVFIRAPWVEQVGPAVKILATV
                     GGDAGAGRIVAVRQGELLATSFHPELTGDLRVHRHFVQMVRARG"
     gene            complement(1317..>2566)
                     /locus_tag="VLC50_00545"
     CDS             complement(1317..>2566)
                     /locus_tag="VLC50_00545"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HSF25983.1"
                     /translation="RRRRRRFTAAITAAAAVVVLVVGLSAVIRVVTPPAQTVAPPPDS
                     GTAATSAPDSPTAATSAPSPRSAPATGHASDLPAGAAVPAGAAPRSVTTAANGLVYVL
                     AAAPCPGGQCPALLRTTDDGRSWRALPAPDGARVNDVRFANPRDGWLYGAQLWATHDG
                     AAEAATWRRIDLGAEVSVLDLASDGTTVWAFVADCTAGDDLGCIRGRLLAASVGSDEF
                     APVPGATLPGPIFRAQLQIAGSRGTLLVTDGAGPGKTFVRRPGDWTRLPRSGPCAQTP
                     VAVVPPATGPAGGLVALCAADAGAGSVTYVTYRSLDEGTSWTRVGPGLRVSNADSTVA
                     AASLDRLAVAVGTPDLQGGLWVSQDGGATWSQPASLPTSQTGWVWVGAAGGARYLAVP
                     FDPTGSVWVSDSAGRSWTPWALR"
BASE COUNT          329 a          951 c          953 g          333 t
ORIGIN      
        1 tccaccgtga cctccatcga cggcacccac tgcgcctcgg cggagtcgta gtcgaggccg
       61 gcctgctgca gcgccgaccg tacggcgacc aggtcgccgg cctcgctgac tacttcgaac
      121 gcctcgccga ggtcgttgac ctcctcggcg ccggcttcga gcacagccag cagcacgtcg
      181 tcctcggtca gcccgtcggc cttgggcacg atgacgacgc ccttgcggtg gaagaggtag
      241 gagaccgagc ccgggtcggc catcgacccg ccgttgcggg tcaccgccac gcgtacgtcg
      301 ctggccgccc ggttgcggtt gtcggtcagg cactcgatga gcagcgccac tccgccggcg
      361 gcgtagccct cgtaggtgat ggtctgccag tcggcgccgc tgccgtcctc gcccgacccg
      421 cgcttgaccg cccggtcgat gttgtcggcc ggcacggagt tcttcttggc cttctggatg
      481 gcgtcataga gcgtcgggtt gccggccggg tcgccgccac cggtgcgcgc cgagacctcg
      541 atgttcttga tcagccgcgc gaacagcttg ccgcgcttgg cgtcgaccac ggccttcttg
      601 tgcttggtgg tggcccactt ggagtggccg gacatcggtt ctccttctcg atcgggcgac
      661 gatggcgccc ctggtcagcc tcgggcgcgc accatctgga cgaagtgccg gtgcacccgc
      721 aggtcaccgg tcagctccgg atgaaacgac gtggccagca gctcgccctg gcggacggcc
      781 acgattctac cggcgccggc atcgcccccc acggtggcga ggatcttcac cgccgggccg
      841 acctgctcca cccacggcgc cctgatgaac accgcgtgga ccgggccgcc gtcgacgccg
      901 gccatctcga gctcgccctc gaacgagtcg acctggcggc cgaacgcgtt gcgccgcacc
      961 gtcatgtcga tgccgccgaa ggtctgctgg tcaggcggtg cgtcaaggac ccggtcagcc
     1021 aacatgatca tcccggcgca cgagccgaac gccggcatcc cctcgcccag ccgcttgcgc
     1081 agcggctcgt cgaggtcgaa ggccgcggcc agcttgtgca tggtggtcga ctcaccgccg
     1141 gggatgacca gcgcgtcgac ggtgtcgagc tcagagggac gtcgtacggc gctcgaggcc
     1201 acgccgagcg cggccagcgc ggcgacgtgc tcgcgcacgt cgccctgcag ggccagcacg
     1261 ccgatgaccg gggcggaggg ggaaggcacg agcagccagt ctaggcgccg ggcggctcag
     1321 cgcagcgccc acggcgtcca gctgcgcccg gccgagtcgc tcacccacac cgagccggtc
     1381 gggtcgaacg gcacggccag gtagcgcgct cccccggccg cgccgaccca gacccagccg
     1441 gtctgagagg tcggcagcga cgccggttgg ctccaggtcg ccccgccgtc ctggctgacc
     1501 cacaggccgc cctgcaggtc gggcgtcccg accgccacgg cgagccggtc gagcgaggcc
     1561 gcggcgaccg tcgagtccgc gttggacacc cgcagccctg ggccgacgcg ggtccagctg
     1621 gtgccctcgt cgagcgagcg gtaggtcacg taggtgacgc tgccggcacc ggcgtcggcg
     1681 gcgcacaggg cgacgagacc gccggccggg ccggtggccg gcgggaccac ggcgacaggg
     1741 gtctgggcac aggggccgga gcgcggcagt cgggtccagt ctccgggccg gcggacgaag
     1801 gtcttcccgg gtccggctcc gtctgtgacc aggagcgtgc cgcgcgaccc ggcgatctgt
     1861 agctgggctc ggaagatcgg tccgggcagg gtcgcccctg ggaccggcgc gaactcgtcg
     1921 ctgcccacgg aggcggcaag cagccgcccc cggatgcagc cgaggtcgtc gccggccgtg
     1981 cagtcggcga cgaaggccca cacggtggtt ccgtcggagg ccaggtcgag cacgctgacc
     2041 tcggcgccga ggtcgatacg ccgccaggtc gccgcctcgg ccgccccgtc atgggtggcc
     2101 cacagctgcg cgccatagag ccagccgtcg cgtgggttgg cgaagcgtac gtcgttgacc
     2161 cgcgcaccgt cgggggcggg cagcgcccgc cagctgcgtc cgtcgtcggt cgtgcgcagc
     2221 agcgccgggc actgcccgcc cgggcagggc gctgcggcca gcacgtagac gaggccgttg
     2281 gcggcggtgg tcaccgaccg cggcgccgcg ccggccggga ccgctgcccc ggcgggcagg
     2341 tcgctggcgt gcccggtggc cggcgcagag cgcgggctcg gcgcgctcgt ggcggcggtg
     2401 ggcgagtcgg gcgcgctggt cgccgcggtg ccggagtccg gcggcggggc cacggtctgc
     2461 gcaggcggcg tcacgacgcg gatcaccgcc gagagaccca ccacgagcac caccacggcg
     2521 gcggccgccg tgatcgccgc cgtgaaccgc cggcggcgcc gccgac
//
LOCUS       DATIBB010000018        13509 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_1717859, whole genome shotgun sequence.
ACCESSION   DATIBB010000018 DATIBB010000000
VERSION     DATIBB010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 13509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13509
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1717859"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1302
                     /locus_tag="VLC50_00550"
     CDS             <1..1302
                     /locus_tag="VLC50_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013678263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HSF25984.1"
                     /translation="DPVVVRALSGLYGPAAAGIDAANNEIIRRLDTGVPMLHEVRPAG
                     EVVPGLDLDARVLLHCGPRIDWARVCDPLRRSMRAAVVAEGWADDVAGADRLLADGEV
                     ALEPANLHSAVVPMATSIGPSAPVFVVRMDEGDTTAYSAVNQGPGNVAWFGRDTDEAI
                     ARLVLLRDVVGPALADVLASSGPLDVFSLAGQGVQMGDDVHMRTQASTNLFIRAMLPH
                     LVELDAAVRRPVAEFLSSNHLFFLNIAMAAAKSLTLWAEQVDGGSIITTMSRNGTDFG
                     VRLAGSDTWHITDAPPIGDALYYADQGPETSAPDIGDSAVLELVGLGGPAAAGSPAVA
                     AFLGGTMADAARATEEMAAICVAGSTKFKLPTLSFRGTPLGVDVRRVVELGITPKVNT
                     GILHASAGTGQVGAGVATAPVECFREALLDLTRRVAPAYAA"
     gene            complement(2348..2983)
                     /locus_tag="VLC50_00555"
     CDS             complement(2348..2983)
                     /locus_tag="VLC50_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013678220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HSF25985.1"
                     /translation="MARKVATPPHEAGATVVSEGPGASLDEVVGGIGPKLRELRQSRG
                     LSLQQLAALAQVSAAAIHKVERGDMVPTITTLLKLSAALNRPVGYFIDELAEPFEVAT
                     HVPADQRPRLDSTLPGLRLEGLSGPSARFRLRAELATIEPGTASEDALPHNGGEALMI
                     VLEGTLAVAVQGRDYLLQAGDSLHFPTDRTHRWVNPGTEPVRAISVSLADR"
     gene            3089..4426
                     /locus_tag="VLC50_00560"
     CDS             3089..4426
                     /locus_tag="VLC50_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013678274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HSF25986.1"
                     /translation="MAFSTVVIGGGPAGLGAAGGIKAVDPANKVTVYTQFEDVAYSPC
                     GIPYVHGKEIDDFQRLFLAGKQAYVDAGIDIHYNSTVTAIDVTRKVVTVEGEGEVPYD
                     RLVIGTGFSYADPGVPGGDLAGLYYVKNIRAAMEWDKVLDSVKRAVVVHATPLGLEMV
                     TALAHRGIETHVIDPNPWAMAEVADPDIMEPVQESWAELGVHMHWNTDLQAFLGNGKI
                     EAVKTSTGDIPVDLAVVATHKVANNALAVKAGLKAGGTGGLVVDERMATSAKDVYAAG
                     DVAELPHGLTRVGLQGLTGSHAYAQGKVAGTNAGGGSRTYQPVYVPWGTPAGKWVIGG
                     ASFGETTATALGIPYVLAVGEGISRARYYPGSKKIKVKLLAEPGTLRLIGAQMVGQEG
                     IKERADLLAMAVKFGLTLHDLSTMENVYSPAIGALAEPIATAATNGINALRKA"
     gene            4729..5214
                     /locus_tag="VLC50_00565"
     CDS             4729..5214
                     /locus_tag="VLC50_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013678272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSMEG_0572/Sll0783 family nitrogen starvation
                     response protein"
                     /protein_id="HSF25987.1"
                     /translation="MAQIVGESVNAGDAIVDYDDKVFPDHQAEPGQKAFIFYHAIPFE
                     SSASLVNLLTATRILRKGFDTSIVFFGPGSLVTAKTRGYPKVGDEGFPGNLTYNKQLQ
                     TFMNEGGKVYACRFSAAALYGMREIDMMDGVIPVNPRDILDATITAWKERALVLNTWT
                     V"
     gene            5383..6192
                     /locus_tag="VLC50_00570"
     CDS             5383..6192
                     /locus_tag="VLC50_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003985147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA family kinase"
                     /protein_id="HSF25988.1"
                     /translation="MLTRVTATRYVAALREGGSLPGLVEADDLGTYVVKFRGAGQGRK
                     ALVAEVIAGELARRLDLPVPELVLVDLDPELSRAEPDEEVQDLLRASAGLNLGVDFLP
                     GALGYDGLGWRPDAELAARVVWFDALVSNADRSWRNPNLLVWHRRLWLIDHGATLWFH
                     HSWPNGVAGGRGAGTPAGAGDRSGDGAATRPYDASEHALTGLVGDLRGADAALAPRVT
                     TALLREVVDLVPDEWLVDDPGNDDPDAVRAAYVEHLLRRVSAPRDWLGVAA"
     gene            6189..6608
                     /locus_tag="VLC50_00575"
     CDS             6189..6608
                     /locus_tag="VLC50_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019434671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3037 domain-containing protein"
                     /protein_id="HSF25989.1"
                     /translation="MSRPSGSSEPRIPFEYAVVRVVPHVERGELVNAGVVVYAQARAY
                     LGCDWHVDEARLRALSPTVDVEGVRAALRAIELVCAGGADAGPAGGGAPGERFRWLVA
                     PRSTVVQPGPVHTGLTTDPAAELAHLMRCLVLVPPPA"
     gene            6629..7294
                     /locus_tag="VLC50_00580"
     CDS             6629..7294
                     /locus_tag="VLC50_00580"
                     /inference="COORDINATES: protein motif:HMM:NF024859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HSF25990.1"
                     /translation="MSQPLARPVAVLDHLVLAAGPDGLQAMVEPLTQRLGLAPTPGGV
                     HPGLGTTNAIVALADGVYLEVVAVADQAAAARSSFGRAVAARQHAGGGWVGWAARLPT
                     YDALADAAAGAGLALADGERTRPDGRTLRWRTAGFDRIEDEPVLPFLIAWDVDDDDHP
                     SHVAPDIARPPRVRALELAGDPHALRRRLGGELAGVRLSWVGITGGLRAVELDVAGAL
                     VAL"
     gene            7305..7979
                     /locus_tag="VLC50_00585"
     CDS             7305..7979
                     /locus_tag="VLC50_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007922088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF25991.1"
                     /translation="MARSRPVLAVYLEQGAKKTFACAADWPGWCRSGKTDDAAIATLL
                     AYADRYAAVAAEAGLARPRVDAAAGGEGVEVLERVAGSATTDFGAPGSVSELDRADLD
                     EAGAERLVALLQASWVLFDRVVAGAPAELRKGPRGGGRDRDKIVEHVLGAEAGYARQI
                     GVRRPQPDPADRAAIEELRAALVAAVTHPDAETSGPKRWPPRYAVRRIAWHVLDHAWE
                     IEDRAG"
     gene            complement(7986..8489)
                     /locus_tag="VLC50_00590"
     CDS             complement(7986..8489)
                     /locus_tag="VLC50_00590"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018547581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HSF25992.1"
                     /translation="MTLHVCFVCTGNICRSPMAEHILRHRLEQEGLGGAVIVDSAGLG
                     GWHAGEPADPRTVSALTAHGYPSEHVARQWQPDWFADRDLVIALDGEHARELRLGAPD
                     GEAADKVVLLREFDPGATDDDLDIPDPYYGGSRGFDDVLAMIETAVDGLLEHLRAELA
                     EPDVSAS"
     gene            complement(8538..8918)
                     /locus_tag="VLC50_00595"
     CDS             complement(8538..8918)
                     /locus_tag="VLC50_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019767207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HSF25993.1"
                     /translation="MRTFLVRLLVNAVALWVAAIVVSGVTLAEGATTSNKIITVLLVA
                     LVFGLVNALIKPFVKLLALPLFILTLGLITFVINALMLMLTSWISGGVDLAFHVDGFG
                     AALLGALVISIVSFVLNVLVPDHP"
     gene            9030..10139
                     /gene="hisC"
                     /locus_tag="VLC50_00600"
     CDS             9030..10139
                     /gene="hisC"
                     /locus_tag="VLC50_00600"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019432237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HSF25994.1"
                     /translation="MTSAQPVTPGPSGPRLRPALDDVPAYKPGRPPAPRPGVTTYKVS
                     SNENPYPPLAGVVEAAAGAVTSMNRYPDLFARELTAELAARFDVPQSHLATGTGSVGV
                     LQQLIQATAGPGDEVVFAWRSFEAYPIVVGIAGATSVTVPLTADERHDLPAMAAAVTD
                     QTRLVLVCSPNNPTGPVVHHDEMVDFLNRVPSDVLVVIDEAYTEFVRDPAAADGIALY
                     RDRPNVAVLRTFSKAYGLAGLRVGFAIAHEPVAEALRKTAVPFGVSTVAQRAAIASLH
                     AEPALRERVEALVQERGRVVAGLTAAGWELASTEANFVWLRLGADTPEFAAACEDAGI
                     MVRPFGEEGVRVTIGEPEANDVLLRVAADWRAARS"
     gene            complement(10140..11096)
                     /locus_tag="VLC50_00605"
     CDS             complement(10140..11096)
                     /locus_tag="VLC50_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020524673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HSF25995.1"
                     /translation="MTTLTRRPARAATPRRVRSTGLMVWVALGIVYLFWGSTYLGIRV
                     MVEDMPPLSAAGSRFVVAGLVLTAVLVVVKGRRVMRIDRRQLAGTALLGLLLPACGNG
                     LMTIGEQSVASGIAALLAAGVPVWVVLLRTASGDRPRGLTWLGVLLGLGGLVVLVARS
                     GSGGASSTIGVLTILLATLGWSLGSWWQGRLTLPANPFVTTVYEMLWGGAFMLAGGWL
                     TGERVDLGAYATSSWVAWSYLVVFGSLVGFSAYVWLLGQAPLSLVATYAYVNPVVAVV
                     LGALILAEPVTGTVLLGGAIVVAGVAVVVSAERPGRAHRQTT"
     gene            11175..12635
                     /locus_tag="VLC50_00610"
     CDS             11175..12635
                     /locus_tag="VLC50_00610"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014379091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HSF25996.1"
                     /translation="MSGMDEREYDVVVVGAGPVGENVVDRVRAAGLSVAVVEERLVGG
                     ECSYFACVPSKALLRPVLAAAATGRVRGLTPARVDPPGVFARRDWWTGGGDDSAQVDW
                     LASTGAELIRGHARLDGPRRVVVETDAAADEAGPAGPAGARVALVARHAVVLATGSSP
                     ALPPIDGLAAARPWTNRQATNASTVPRRLAVLGGGVVGAEMATAYAGLGAKVTVLARG
                     PRLLGRMEPFAGELVADGLREAGVDVRLRTTVTAVHRQADGTVTVSTDGGETVRADQV
                     LAALGRRPNTATLGVDTVGLKPGESVEVDDSLRATGVEDGWLYATGDVNGRNLLTHMG
                     KYQARAAADVIVARATGKPDDAAGMRAGSDAGAVTQVIFTDPEVAAVGPTLEQAREQA
                     GADGRVRAVDVEMSAASGAGLQADGYRGKARLVLDEDRRVVLGATFVGQDVAELLHAA
                     TIAVVGQVPLDALWQAVPAFPTMSEVWLRLLESAGL"
     gene            12901..>13509
                     /locus_tag="VLC50_00615"
     CDS             12901..>13509
                     /locus_tag="VLC50_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019874329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HSF25997.1"
                     /translation="MGPEHPELVQLLTPEGERIEHPDYAFDLDDEAIKGFYRDLVLTR
                     RIDIEATALQRQGQLGIWASLLGQEAAQIGSGRALLPQDYAFPTYREHGVAWCRGVDP
                     MRLLGLFRGVDQGGWDPREHNFHLYTIVIGAQALHATGYAMGIQRDGLVGTGDDTRDA
                     ASIVYFGDGATSQGDVHESFVFAGVYNAPVVFFCQNNQWAISE"
BASE COUNT         1897 a         4851 c         4938 g         1823 t
ORIGIN      
        1 gaccccgtcg tcgtacgtgc cttgtccggt ctctacggcc cggccgccgc cggcatcgac
       61 gcggccaaca acgagatcat ccgccggctc gacaccgggg tgccgatgct gcacgaggtc
      121 cgccccgccg gcgaggtggt gcccgggctg gacctcgacg cgcgggtgct gctgcactgc
      181 gggccccgca tcgactgggc ccgggtctgc gacccgctgc gccggtcgat gcgggccgcg
      241 gtggtggccg agggctgggc cgacgacgtg gccggggccg accggctgct cgccgacggc
      301 gaggtcgcgc tcgagccggc caacctgcac agcgcggtgg tgccgatggc cacctcgatc
      361 ggcccgtcgg cgccggtctt cgtcgtacgg atggatgagg gcgacaccac cgcctactcg
      421 gcggtcaacc aggggccggg caacgtcgcc tggttcggcc gcgacaccga cgaggcgatc
      481 gcccggctgg tgctgctgcg cgacgtggtt ggcccggcgc tggccgacgt tctcgcgtcg
      541 tcgggaccgc tggacgtgtt ctcgctggcc gggcagggcg tgcagatggg cgacgacgtc
      601 cacatgcgca cccaggccag caccaacctg ttcatccgcg cgatgctgcc ccacctcgtc
      661 gagctcgacg ccgcggtgcg ccgtccggtg gcggagttcc tcagctccaa ccacctgttc
      721 ttcctcaaca tcgcgatggc cgcggccaag tcgctgacgc tgtgggccga gcaggtcgac
      781 ggcggcagca tcatcacgac gatgtcgcgc aacggcaccg acttcggggt gcggctggcc
      841 ggctccgaca cctggcacat caccgacgcc ccgccgatcg gggacgcgct ctactacgcc
      901 gaccaaggcc ccgagaccag cgcgccggac atcggcgaca gcgcggtgct cgagctcgtc
      961 gggctgggcg gaccggccgc ggccgggtcg ccggcggtgg cggcattcct cggcggcacc
     1021 atggccgacg cggcccgggc caccgaggag atggccgcga tctgcgtggc cgggagcacg
     1081 aagttcaagc tgccgacgct gtccttccgc ggcaccccgc tcggggtcga cgtccgccgg
     1141 gtggtcgagc tgggcatcac gcccaaggtc aacaccggca tcctgcacgc cagcgcgggc
     1201 accgggcagg tcggcgccgg ggtggcgacg gcgccggtcg agtgcttccg cgaggcgctg
     1261 ctcgacctga cccgccgggt ggcccccgcc tacgccgcct gatccgcccc gtcaccaccc
     1321 atcagcgatc aagcaatcga tgtacggcga gcgcgctcgc ccgagccttc gcggtgccgt
     1381 gccagcagtt gtcgcgctga agaacggcac ctaccggacg atcgggcggt gtcggaatcg
     1441 ctttgcggcc caggtcccga gcgagagcat cccgagaccc aggcaagcaa tgaagatcat
     1501 cgtcgcgatc tcgaacgtcc agtttgagcc gctctgcgag aggtcgtgag cacgggaagg
     1561 gtgctgcggg tcgtagtcga caatgacggg tgatccaatc ggcggccgcg aattgccgtt
     1621 cggcgcagtg aacgcatgcc gtgtcccacc gctgtcgacg aagctgatgg tggccgcata
     1681 gacacacccg cgaaagcatt gcgtggagac gctctggact gccccattgg tgcggatcca
     1741 ccccggggtc gaacgcagca gtgaccgctc atggaatccg tgggcagctc caagtgcgcc
     1801 agcgagcacg agcgccaagc cgatgagaag gacagccgta atgcctgccc gcccgactct
     1861 ctttccccta acgggacccg gggcggttgc cgacgcctct tccacggcct catcgtccca
     1921 gccaagtggc gactgcaacg ccggttctgt tccttcatga caattttatg cgcccgaccg
     1981 aacccggccg ccggttcgga gcggcaggcg cactcccgtg tgcggagtga ggttttcagc
     2041 aatcgccacc acaccagcgc ggcagcctcg gcgtccgaac ctgcccggtg cgcgggtcca
     2101 gcgcgttcga cgctgagttg atgtccagct ggccggcgac cgcccgaacg ccgggcctcg
     2161 acgaggtcgc cgtcgcggat gccatgcaca cgcgtcgcgc cgagcggccg ccacggacgc
     2221 acagtgtctg cctgcccgac acgcagccat cgtcctgcgt cactcgcgag tgtgctcacc
     2281 ttgcccgacg ccgcaatcgt agtgatcttg ggccgcggac cggatctagg tcacctccat
     2341 gcgacggtca gcggtcggcg agggagaccg agatcgcgcg gaccggctcg gtgcccgggt
     2401 tgacccagcg gtgggtgcgg tcggtgggga agtgcaggct gtcgccggcc tgcaggaggt
     2461 agtcgcggcc ctgcaccgcc accgccaggg tcccctcgag cacgatcatg agcgcctcgc
     2521 caccgttgtg cggcagggcg tcctcgctcg ccgtaccggg ctcgatcgtg gccagctcgg
     2581 cccgcagccg gaaccgggcg ctcgggccgg acagcccctc cagccggagg ccgggcaggg
     2641 tcgagtcgag acgtggacgc tggtcggcgg gcacgtgcgt ggcgacctcg aacggctcgg
     2701 ccagctcgtc gatgaagtag ccgaccgggc ggttgagcgc cgccgagagc ttgagcagcg
     2761 tggtgatcgt ggggaccatg tcgccgcgct cgaccttgtg gatggccgcg gccgagacct
     2821 gggccagcgc ggccagctgc tgcagcgaga gcccccgcga ctggcgcagc tcgcgcagct
     2881 tgggcccgat cccgccgacc acctcgtcga ggctcgcgcc gggcccctcg gacacgacgg
     2941 tcgcgcccgc ttcgtgcggc ggagttgcga ccttccgggc catctcggtg ctccttgaca
     3001 gtgggtagac gtgcaatata gtaaacggcg ctgacgatag tagatgcggc tccgttcgca
     3061 ccggtcatgg tgccagaagg gacatcgcgt ggcgttttcg accgtggtca tcggcggtgg
     3121 gccggccggg ctgggcgcag cgggcggcat caaggccgtc gatccggcca acaaggtcac
     3181 cgtctacacg cagttcgagg acgtcgccta ctccccgtgc gggatcccct acgtccacgg
     3241 caaggagatc gacgacttcc agcggctgtt cctggccggc aagcaggcgt acgtcgatgc
     3301 cggcatcgac atccactaca acagcaccgt caccgcgatc gacgtgaccc gcaaggtggt
     3361 cacggtcgag ggcgagggcg aggtgcccta cgaccggctg gtgatcggga ccggcttcag
     3421 ctacgccgac cccggggtgc ccggcggcga cctggccggc ctctactacg tcaagaacat
     3481 ccgcgcggcg atggagtggg acaaggtcct cgactcggtc aagcgcgcgg tcgtcgtaca
     3541 cgccaccccg ctgggcctcg agatggtcac ggcactggcc caccgcggca tcgagaccca
     3601 cgtcatcgac cccaacccgt gggcgatggc cgaggtcgcc gaccccgaca tcatggagcc
     3661 ggtccaggag tcgtgggccg agctcggcgt gcacatgcac tggaacaccg acctgcaggc
     3721 cttcctcggc aacggcaaga tcgaggcggt caagacgtcc acgggcgaca ttcccgtcga
     3781 cctcgcggtc gtggccacgc acaaggtcgc caacaatgcg ctcgcggtca aggccgggct
     3841 caaggccggc ggcaccgggg ggctggtggt cgacgagcgg atggccacct cggccaagga
     3901 cgtgtacgcc gccggtgacg tggccgaact cccgcacggg ctgacccggg tcgggctgca
     3961 ggggctgacc ggcagccacg cctacgcgca gggcaaggtc gccggaacca atgccggtgg
     4021 cggctcgcgc acctaccagc cggtctatgt cccgtggggc accccggcgg gcaagtgggt
     4081 gatcgggggc gcgtcattcg gcgagaccac cgcgaccgcg ctcggcattc cctacgtcct
     4141 ggccgttggt gagggcatct cgcgggcacg ctactacccg ggctccaaga agatcaaggt
     4201 caagctgctc gccgagcccg gcacgctgcg gctcatcggg gcccagatgg tcggtcagga
     4261 aggcatcaag gaacgcgccg acctgctcgc gatggcggtg aagttcgggc tgaccctcca
     4321 cgacctgtcg accatggaga atgtctactc cccggcgatc ggggcgctgg ccgaaccgat
     4381 cgcgaccgcg gccaccaacg gcatcaacgc cctgcggaag gcctgaggtg tcattcagca
     4441 gctggcttcg cgagaatgcc gagcactact tgctcacttc cgcccaggac caggtggcca
     4501 gcgcgtccgg ctacaaccgg cctcgtccgc cacgcggtcc cgtccagctg ttctggttgc
     4561 gggtcttcgc tcccatttat cgaactctcc cctggcgcct gcgacagcgt gtcatggtca
     4621 cgatgcctgg cagtcatcgc aaggcgtgga cgtgggaacc aacacggcgg ggacccgctg
     4681 tgtgaacgtc gtaccagagg caccagacca tctactagga ggacagctgt ggctcagatc
     4741 gtgggcgaga gtgtcaacgc gggcgacgcc atcgtcgact atgacgacaa ggtgtttccc
     4801 gaccaccagg ccgaacccgg ccagaaggcg ttcatcttct accacgccat tccgttcgag
     4861 agctccgcgt cgctggtcaa cctgctgacg gcgacccgga tcctgcgcaa gggctttgac
     4921 acgagcatcg tgttcttcgg ccccggctcg ctggtcaccg ccaagacgcg cggctacccg
     4981 aaggtcggcg acgagggctt cccgggcaac ctcacctaca acaagcagct gcagacgttc
     5041 atgaacgagg gcggcaaggt ctacgcctgc cggttctccg ccgcggcgct ctacggcatg
     5101 cgtgagatcg acatgatgga cggcgtcatc ccggtcaacc cccgcgacat cctcgacgcg
     5161 acgatcacgg cgtggaagga gcgcgcgctc gtcctcaaca cctggacggt gtgagcgcgg
     5221 cacgtcactg acggacgcac gccccctggg tccccagggg gcgtgcgcac gggctcggcg
     5281 atctgttccc gcagcggtca ggcgcattct cggtacgacg ggaggcggct gatcgctgcc
     5341 gtgcgttgtt gagggtccct cgcgcgtgcg tagcctgtca gcgtgctgac ccgggtcacg
     5401 gccacgcgct acgtcgccgc gctgcgtgaa ggcgggtcgc tgcccgggct cgtcgaggcc
     5461 gacgacctgg gcacctacgt cgtgaagttc cgcggcgccg ggcagggccg caaggcgctc
     5521 gtcgccgagg tgatcgccgg cgagctggcc cgccggctcg acctgccggt gccggagctg
     5581 gtgctcgtcg acctcgaccc cgagctgtcc cgggccgagc ctgacgagga ggtgcaggac
     5641 ctgctgcggg ccagcgccgg gctcaatctc ggcgtcgact tcctcccggg ggcgctgggc
     5701 tatgacgggc tgggctggcg ccccgacgcc gagctggccg cccgggtcgt ctggttcgac
     5761 gcgctggtct ccaacgccga ccgcagctgg cgcaacccca acctgctggt ctggcaccgg
     5821 cggctgtggc tgatcgacca cggcgcgacg ctgtggttcc accactcctg gccgaacggc
     5881 gtggccggcg ggcggggggc cggcacgccc gccggcgccg gtgacaggtc gggcgacggt
     5941 gcggcgacgc ggccgtacga cgccagcgag cacgcgctga ccgggctcgt cggtgacctg
     6001 cgcggcgccg acgccgcgct ggccccccgg gtcaccacgg cgctgctgcg cgaggtggtc
     6061 gacctggtgc ctgatgagtg gctggtcgac gacccgggca acgacgaccc cgacgccgtg
     6121 cgcgcggcct acgtcgagca cctgctccgg cgggtctcgg cgccgcggga ctggctgggg
     6181 gtggcggcat gagccggccg agcgggtcga gcgagccgcg gatccccttc gagtacgccg
     6241 tggtccgggt cgtgccgcac gtcgagcgcg gcgagctggt caacgccggg gtggtcgtct
     6301 acgcccaggc ccgcgcctac ctcggctgtg actggcacgt cgacgaggcg cggctgcgcg
     6361 cgctgtcgcc gaccgtggac gtcgagggcg tgcgcgcggc gctgcgcgcg atcgagctgg
     6421 tctgcgccgg cggcgccgac gccggcccgg ccggcggggg cgcgccgggt gagcggttcc
     6481 gctggctggt cgccccccgc agcaccgtcg tgcagccggg cccggtgcac accgggctga
     6541 ccaccgaccc ggccgccgag ctggcccacc tgatgcgctg cctcgtgctg gtgccgcctc
     6601 cggcctaggc tcgggccggg agggatccgt gtcgcagccg ctcgctcgtc ccgtcgccgt
     6661 gctcgaccat ctcgtgctgg ccgccggccc ggacggcctg caggccatgg tcgaaccgct
     6721 cacccagcgg ctcggcctgg cgcccacacc gggtggcgtg catcccgggc tgggcaccac
     6781 caacgcgatc gtggcgctgg ccgacggggt ctacctcgag gtggtggcgg tcgccgacca
     6841 ggcggccgcg gcccggtcgt cgttcggccg ggcggtggcg gcccgtcagc atgccggcgg
     6901 tggctgggtg ggctgggcgg ctcggctccc gacgtacgac gcgctggccg atgccgcggc
     6961 cggggcgggg ctggccctgg ccgacgggga gcgcacccga ccggacgggc gcacgctgcg
     7021 ctggcgcacc gcggggttcg accggatcga ggacgagccg gtgctgccgt tcctcatcgc
     7081 atgggacgtc gacgacgacg accacccgtc gcacgtcgcc cccgacatcg cgcgtccgcc
     7141 gcgggtgcgg gcgctcgagc tggccggtga cccgcacgcg ctgcgccgcc ggctcggcgg
     7201 cgagctggcc ggggtccggc tctcctgggt gggcatcacc ggggggctgc gggcggtcga
     7261 gctcgacgtg gccggtgcgc tggtggcgtt gtgaggaggc cgtgatggca cgctcgcgcc
     7321 ccgtgctggc tgtctacctc gagcaggggg ccaagaagac cttcgcctgc gcggccgact
     7381 ggccgggctg gtgccgctca ggcaagaccg acgacgcggc gatcgccacg ctgctcgcct
     7441 acgccgaccg ctacgccgcg gtggccgccg aggccggcct ggcgcgcccc cgcgtcgacg
     7501 ccgccgccgg aggtgagggg gtcgaggtgc tcgagcgggt cgccgggtcg gcgaccaccg
     7561 acttcggcgc cccgggcagc gtgagcgagc tggaccgggc cgacctcgac gaggcgggcg
     7621 ccgagcggct ggtcgcgctc ctccaagcgt cgtgggtgct tttcgaccgg gtcgtcgccg
     7681 gggcgccggc cgagctgcgc aagggccccc ggggcggtgg ccgcgaccgg gacaagatcg
     7741 tcgagcacgt gctcggtgcc gaggcgggct acgcccgcca gatcggcgta cgacgccccc
     7801 agcccgaccc cgccgatcgg gcagcgatcg aggagctgcg cgcggcgctg gtcgccgcgg
     7861 tcacccaccc cgacgccgag acctccggcc ccaagcggtg gccgcctcgc tacgccgtac
     7921 ggcggatcgc ctggcacgtg ctcgaccacg cctgggagat cgaggaccgt gctggttagc
     7981 gccgactagc tggccgacac gtccggctcg gccagctcgg ctcgcaggtg ctcgagcagc
     8041 ccgtcgacgg cggtctcgat catcgccagc acgtcgtcga acccgcgcga cccgccgtag
     8101 taggggtcgg ggatgtcgag gtcgtcgtcg gtggccccgg ggtcgaactc gcgcagcagc
     8161 acgaccttgt cggcggcctc cccgtcgggg gcgccgaggc gcagctcgcg ggcgtgctcg
     8221 ccgtcgaggg cgatgaccag gtcacggtcg gcgaaccagt cgggctgcca ctgccgggcg
     8281 acgtgctcgc tcggatagcc gtgcgcggtc agcgcgctga ccgtgcgcgg gtcggccggc
     8341 tcgcccgcgt gccagccgcc cagaccggcg ctgtcgacga tgaccgcacc gcccaacccc
     8401 tcctgctcga gccggtggcg caagatgtgc tcggccatcg gcgagcgaca aatgttgcca
     8461 gtgcagacga agcagacgtg aagggtcacg ggcgacatac tggccgccta ggccgatcgc
     8521 gtcgaccccc gggcgcgcta ggggtggtcg ggcacgagca cgttgagcac gaagctgacg
     8581 atcgagatga ccagcgcgcc gagcagggcg gcgccgaagc cgtcgacgtg gaaggccagg
     8641 tcgacgcccc cggagatcca cgaggtgagc atcagcatca gcgcgttgat gacgaacgtg
     8701 atcaggccca gggtcaggat gaacaacggc agcgcgagca gcttgacgaa cggcttgatc
     8761 agcgcgttga ccaggccgaa caccagcgcg accagcagca ccgtgatgat cttgttggag
     8821 gtggtggccc cctcggccag ggtcacgccg ctgaccacga tcgcggcgac ccagagcgcg
     8881 acggcgttga ccagcaggcg cacgaggaag gtccgcatgg cccgatcgtg gcagacccgg
     8941 cggtgactgt cgcgcgaccc gcggcacccg gatgccgggc gggtcggcac ccggatgcct
     9001 gggcgggtcg ccgggtaacg ttcgacgcca tgacgtcagc ccagcccgtc acccccggtc
     9061 cgtcgggtcc tcgcctgcgc cccgcgcttg acgacgtgcc cgcctacaag cccggccgcc
     9121 cgcccgcgcc gcgccccggt gtgacgacct acaaggtctc gtccaacgag aacccctacc
     9181 cgccgctggc cggcgtggtc gaggccgcgg ccggtgcggt cacgtcgatg aaccgctacc
     9241 ccgacctgtt cgcgcgcgag ctgaccgccg agctggccgc tcgcttcgac gtgccgcagt
     9301 cgcacctggc caccggcacc ggcagcgtcg gggtgctgca gcagctgatc caggcgaccg
     9361 ccgggccggg cgacgaggtg gtcttcgcgt ggcgctcgtt cgaggcctac ccgatcgtgg
     9421 tcggcatcgc cggcgccacg tcggtgacgg tgccgctgac cgccgacgag cgccacgacc
     9481 tgccggcgat ggccgccgcg gtcaccgacc agacccgcct ggtgctggtc tgctcgccca
     9541 acaacccgac cggcccggtc gtgcaccacg acgagatggt cgacttcctc aaccgggtgc
     9601 cctccgacgt gctcgtcgtc atcgacgagg cctacaccga gttcgtccgc gacccggccg
     9661 ccgccgacgg catcgcgctc tatcgcgacc ggcccaacgt cgccgtgctg cgcacgttct
     9721 ccaaggccta cgggctggcc gggctgcggg tgggcttcgc gatcgcgcac gagccggtgg
     9781 ccgaggcgct gcgcaagacc gcggtgccgt tcggggtctc gacggtggcc cagcgggccg
     9841 ctatcgcgtc gctgcacgct gagccggcgc tgcgggagcg ggtcgaggcg ctggtgcagg
     9901 agcgcggccg ggtggtggcc gggctgaccg ccgccggctg ggagctggcc tcgaccgagg
     9961 ccaacttcgt ctggctgcgg ctgggcgccg acaccccgga gttcgccgcc gcctgcgagg
    10021 acgccgggat catggtgcgc ccgtttggcg aggagggcgt gcgggtgacc atcggcgagc
    10081 ccgaggccaa cgacgtgctg ctgcgggtgg ccgccgactg gcgcgccgcc cgcagctgat
    10141 caggtggtct ggcggtgcgc ccggccgggg cgttcggcgc tgaccacgac cgcgaccccg
    10201 gccaccacga tcgccccgcc gagcagcacg gtgccggtga ccggctcggc caggatcagc
    10261 gcgccaagga ccaccgcgac gaccgggttg acgtaggcgt aggtggccac cagcgacagc
    10321 ggcgcctggc cgagcagcca gacgtaggcc gagaacccga ccagcgagcc gaacaccacg
    10381 aggtaggacc aggccaccca cgaggacgtc gcgtaggcgc cgaggtccac ccgttcgccg
    10441 gtgagccagc cgccggccag catgaaggcc ccgccccaga gcatctcgta gaccgtggtg
    10501 acgaacgggt tggccggcag cgtgagccgc ccctgccacc acgagcccag cgaccagccg
    10561 agcgtggcca ggaggatggt cagtacgccg atcgtgctgc tggcgccgcc gctgcccgac
    10621 cgggcgacga gcacgacgag cccgcccagg ccgagcagca cgccgagcca ggtcagcccg
    10681 cgtgggcggt cgccggacgc cgtacgcagc agcaccaccc agaccggcac gccggccgcc
    10741 agcagggcag cgatgcccga ggcgacgctc tgctcgccga tggtcatcag gccgttgccg
    10801 caggcgggca gcagcaggcc gagcagtgcc gtgccggcca gctggcgccg gtcgatccgc
    10861 atcacccgcc gccccttgac cacgaccagc accgcggtca gcaccagccc ggcgacgacg
    10921 aaccggctgc cggccgccga caggggcggc atgtcctcga ccatgacccg gatgccgagg
    10981 taggtcgagc cccagaacag atagacgatg cccagggcga cccagaccat cagcccggtc
    11041 gagcggacgc gccggggcgt ggcggcgcgc gctggccggc gagtcaacgt ggtcatgagt
    11101 gtcctgtcga tttggtgctg acatcgtagt ggcgtacgac gacgtgcggg cgctgccgac
    11161 gagttgaggt cacgatgagc ggcatggacg agcgcgagta tgacgtggtg gtggtcggcg
    11221 ccggaccggt aggggagaac gtggtcgacc gggtccgcgc ggccggcctg tcggtggccg
    11281 tcgtcgagga gcgcctggtc ggcggcgagt gcagctattt cgcgtgcgtg ccgagcaagg
    11341 cgctgctgcg cccggtgctg gccgccgcgg cgaccggccg ggtgcgcggg ctgacccctg
    11401 cccgggtcga cccgcccggg gtgttcgccc gccgcgactg gtggaccggc ggcggtgacg
    11461 acagcgcgca ggtcgactgg ctcgcctcga ccggcgctga gctcatccgc gggcatgccc
    11521 ggctcgacgg cccgcgccgc gtcgtggtcg agaccgatgc cgcggccgac gaggccggcc
    11581 ccgccggccc cgcgggcgcg cgagtcgcgc tggtggctcg ccacgccgtc gtgctggcca
    11641 ccggctcgtc gcccgcgctg ccgcccatcg acgggctggc cgccgcgcgg ccctggacca
    11701 accggcaagc caccaacgcc agcacggtgc cgcggcggct ggcggtgctc ggcggcggcg
    11761 tagtcggtgc cgagatggcg accgcctacg ccggcctcgg cgcaaaggtc acggtgctgg
    11821 cccgcggccc gcgcctgctg ggtcgcatgg agccgtttgc cggcgagctg gtcgccgacg
    11881 ggctgcgcga ggccggtgtc gacgtacggc tccgcaccac cgtcaccgcc gtgcaccgcc
    11941 aggccgacgg cacggtcacc gtcagcaccg acggcggcga gaccgtgcgc gccgaccagg
    12001 tgctggccgc gctggggcgc cggcccaaca ccgccaccct gggcgtggac accgtcgggc
    12061 tcaagccggg ggagagcgtc gaggtcgacg acagcctgcg cgccaccggg gtcgaggacg
    12121 gctggctcta tgccaccggc gacgtcaacg ggcgcaacct gctcactcac atgggcaagt
    12181 accaggcccg cgccgccgcc gacgtgatcg tggcccgagc gaccggcaag ccggacgacg
    12241 cagcgggcat gcgggccggc tcggacgccg gggcggtcac ccaggtgatc ttcaccgacc
    12301 cggaggtggc cgcggtcggc cccacgctgg agcaggcccg cgaacaggcg ggggccgacg
    12361 gtcgggtgcg cgccgtcgac gtcgagatga gcgcggccag cggcgccggg ctgcaggccg
    12421 acggctaccg cggcaaggcc cggctggtgc tcgacgagga ccgccgggtc gtgctcggcg
    12481 ccacgttcgt cggccaggac gtcgccgagc tgctgcacgc cgcgaccatc gcggtggtcg
    12541 ggcaggtgcc gctcgacgcg ctgtggcagg ccgtgccggc tttccccacg atgagcgagg
    12601 tctggttgcg gctgctggag agcgcggggc tatgacctgc ggcgtacggc gcgcggcggc
    12661 tgcgccgtac gggcatgatt cgtcctaggg aggtggtggt taccgagtgg tatcgatgcg
    12721 tcggtaggtt gctgcctagc gatcgtgggt cgtgccacaa tcgccgctgc ccgacccgag
    12781 tcgtggatcg tcgcgcccac cccgcgcggt ggccgcgggc tcggggcgtc cgacccggtc
    12841 gcggagaccc cgccgaccgg tgctgaggcg aggagtgtca cgttgatcga acccagcgcg
    12901 atgggaccgg agcaccccga gctcgtccag ctgctcaccc ccgagggcga gcggatcgag
    12961 caccccgact atgcctttga tctcgacgac gaggcgatca agggcttcta ccgcgacctg
    13021 gtgctgaccc ggcgcatcga catcgaggcc accgccctgc agcggcaggg ccagctgggt
    13081 atctgggcca gcctgctcgg ccaggaggcc gcccagatcg gctccggccg ggcgctgctg
    13141 ccgcaggact acgcgttccc gacctaccgc gagcacggcg tcgcctggtg ccgcggcgtc
    13201 gacccgatgc gactgctcgg gctcttccgc ggcgtcgacc agggcggctg ggacccgcgc
    13261 gagcacaact tccacctcta cacgatcgtc atcggcgccc aagcgctgca cgccaccggc
    13321 tacgcgatgg gcatccagcg cgacgggctg gtcggcaccg gcgacgacac ccgcgacgcg
    13381 gcctcgatcg tctacttcgg cgacggcgcg accagccagg gcgacgtaca cgagtccttt
    13441 gtctttgccg gggtctacaa cgctccggtg gtcttcttct gccagaacaa ccagtgggcc
    13501 atctccgag
//
LOCUS       DATIBB010000019         2669 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_270872, whole genome shotgun sequence.
ACCESSION   DATIBB010000019 DATIBB010000000
VERSION     DATIBB010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2669
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_270872"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1618
                     /locus_tag="VLC50_00620"
     CDS             <1..1618
                     /locus_tag="VLC50_00620"
                     /inference="COORDINATES: protein motif:HMM:NF024594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4012 domain-containing protein"
                     /protein_id="HSF25998.1"
                     /translation="DTPRAEIDRHLRTVQAEARAAHDLTGGPLWDLPARLPWAGRPFE
                     TVQGVAAGVDTVADEVLPPVRSVRDRLVGVDLSRGEGGLDLAPIAAAQTPLAQARTAN
                     DRALRDVSDLPSTGIAPIDEGRRDLVGQLDAVSRQLRSAHEAVELLPPMLGAEGPRRY
                     FVAFQNNAEARGAGGLPGVFAVLRADKGRVSFEQYGVSGDFGGLQVPLTGLSPGYAEH
                     YREAGPGKFFGNATVSPHFPDGAELMLRFWKAKTGQQLDGALAIDPNALAMMLEVTGP
                     TTMADGTVLKASSVVALTEKDAYQRFTDPVVRKQYLITVAKAIADDVLERGPAKGTAL
                     ASAVGRGVGERRILLYSTHAEEQRVIAARPVGGALSDTAGLFSGVVINNGGGNKLDYY
                     LDRQVTYQGPACGAADPRATVTIRLTNRAPKSGLTNYVAGRADLPVDPVPKGTNRLLV
                     GYYATKEAGFSDATLDGEPTVLAVDDERGRPVFTAAFELEPGQTRTLVIRIEEPPEAK
                     GPVTTLVQPLVLPQRTTIEVPDCRRGQETG"
     gene            complement(1638..>2669)
                     /gene="aroB"
                     /locus_tag="VLC50_00625"
     CDS             complement(1638..>2669)
                     /gene="aroB"
                     /locus_tag="VLC50_00625"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013771147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HSF25999.1"
                     /translation="LPRLIGEGVQRVLVIHPRALATTGEAVREDLVAKGFEAYAAEVP
                     DAEEAKTAQVAAFLWGVLGQAGFTRSDAIVSVGGGTVTDLAGFVAATWLRGLRVVHVP
                     TTLLGMVDAAVGGKTGINTPEGKNLVGAFHPPAGVLCDLATLQTLPRHDFVAGLAEVI
                     KCGFIADPVILDLVEGDAEGATTAEGPHVRELVERAIAVKADVVAQDLRESSLREILN
                     YGHTFGHAVEQVERYQFRHGAAVSIGMVYVAELARLAGKIDDDLVDRHRSILSSVGLP
                     VTYRGDRWPRLLEAMKRDKKSRGSMLRFVVLAGLAKPVRLEGPDPALLQAAYAEISAE
                     TAGRAISLG"
BASE COUNT          377 a         1008 c          943 g          341 t
ORIGIN      
        1 ggacaccccg cgcgcggaga tcgaccgcca cctgcggacg gtccaggcgg aggctcgggc
       61 ggcccacgac ctcaccggtg ggcccctgtg ggacctcccg gcgcggctgc cgtgggccgg
      121 ccgccccttc gagaccgtcc agggcgtcgc tgcgggcgtc gacaccgtcg ccgacgaggt
      181 gctgccgccg gtgcgctcgg tcagggaccg cctcgtcggg gtcgacctga gccgcggcga
      241 gggcggcctg gacctcgctc ccattgcggc ggcgcagacg cctctcgccc aggcgcgcac
      301 ggcgaatgac cgggctctgc gagacgtcag cgacctgccg tccacgggca tcgcgcccat
      361 cgacgagggg cggagggacc tcgtcggcca gctcgatgcg gtctcgcggc agctccgctc
      421 ggcccacgag gccgtggagc tgctgccgcc gatgctcggc gccgagggcc cgcgccgcta
      481 cttcgtggcc ttccagaaca acgccgaggc gcgcggcgcc ggcgggctcc ccggcgtctt
      541 cgccgtgctg cgcgccgaca agggcagggt cagcttcgag cagtacggcg tctcaggcga
      601 cttcggcggt ctccaggtcc cgctgacggg cctgagcccc ggctatgccg agcactaccg
      661 cgaggccggg ccagggaagt tcttcggcaa cgccacggtg agcccgcact tccctgacgg
      721 cgccgagctc atgctgcggt tctggaaggc caagaccggc cagcagctcg acggtgccct
      781 ggcgatcgac cccaacgcgc tggccatgat gctcgaggtg accgggccca ccacgatggc
      841 cgacggcacc gtcctgaagg cgtcctcggt ggtggccctc accgagaagg acgcctacca
      901 gcggttcacc gacccggtgg tgcgcaagca gtacctcatc accgtggcca aggccatcgc
      961 cgacgacgtg ctcgagcgcg ggcccgccaa gggcaccgcg ctggcctcgg ccgtcgggcg
     1021 gggggtcggc gagcgtcgca tcctcctcta cagcacccac gccgaggagc agcgcgtgat
     1081 cgccgcgcgg ccggtggggg gcgcgctcag cgacacggcc ggcctcttca gcggcgtcgt
     1141 catcaacaac ggcggcggca acaagctcga ctactacctc gaccgtcagg tcacgtacca
     1201 gggcccggcg tgcggagccg ccgacccgcg ggccacggtc acgatccgcc tcaccaaccg
     1261 cgccccgaag tcgggcctga ccaactacgt cgccggacgc gccgacctac ccgtcgaccc
     1321 tgtgcccaag ggcacgaacc gcctgctggt cggctactac gccaccaagg aggcgggctt
     1381 ctccgacgcc accctcgatg gcgagcccac cgtcctggcg gtcgacgacg agcgcgggcg
     1441 tcccgtcttc accgccgcgt tcgagctcga gccgggtcag acccggaccc tcgtgatcag
     1501 gatcgaggag ccaccggagg ccaagggccc ggtcacgacg ctggtgcagc cgctcgtcct
     1561 gccacagcgc accacgatcg aggtccccga ctgccgccgc ggccaggaga ccggctgacc
     1621 ggcacgcgga acaggcgtca gccgagcgag atcgctcggc ccgcagtctc cgcgctgatc
     1681 tcggcgtagg cagcctgcag cagggccggg tcgggtccct cgagccgcac cggcttggcg
     1741 agtccggcca gcacgacgaa gcgcagcatc gacccgcggg acttcttgtc gcgcttcatc
     1801 gcctcgagca gccgcggcca ccggtccccg cggtacgtca cgggcagccc cacgctgctg
     1861 aggatcgacc ggtgccggtc gacgaggtcg tcgtcgatct tcccggcgag ccgcgcgagc
     1921 tcggcgacgt agaccatgcc gatgctgacc gcggcaccgt gccggaactg gtagcgctcg
     1981 acctgctcga cggcgtggcc gaaggtgtgg ccgtagttga ggatctcgcg cagcgaggac
     2041 tcccgcaggt cctgcgccac gacgtcggcc ttgaccgcga tcgcgcgctc gacgagctcg
     2101 cgcacgtgcg gcccctccgc cgtggtcgcg ccctcggcgt cgccctcgac gaggtcgagg
     2161 atcaccgggt cggcgatgaa gccgcacttg atgacctcgg ccagcccggc gacgaagtcg
     2221 tgccggggca gcgtctgcag ggtggcgagg tcgcacagca cgccggccgg cgggtggaac
     2281 gcgccgacga ggttcttgcc ctcgggggtg ttgatcccgg tctttccgcc gactgccgcg
     2341 tcgaccatcc ccagcagggt ggtgggcacg tgcacgacgc gcaggccccg cagccaggtg
     2401 gccgccacga aaccggccag gtcggtgacg gtcccgccgc cgacggagac gatggcgtcc
     2461 gagcgggtga acccggcctg cccgagcacg ccccagagga aggctgccac ctgcgcggtc
     2521 ttggcctcct ccgcgtccgg cacctccgcg gcatacgcct cgaagccctt ggcgacgagg
     2581 tcctcccgca cggcctcgcc cgtcgtggcg agggcccggg ggtggatcac gaggacccgc
     2641 tgcacccctt cgccgatgag tcggggcag
//
LOCUS       DATIBB010000020        15560 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279 k141_8296,
            whole genome shotgun sequence.
ACCESSION   DATIBB010000020 DATIBB010000000
VERSION     DATIBB010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 15560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15560
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8296"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..967)
                     /locus_tag="VLC50_00630"
     CDS             complement(<1..967)
                     /locus_tag="VLC50_00630"
                     /EC_number="4.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009717335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004122 - cystathionine beta-synthase
                     activity [Evidence IEA];
                     GO_process: GO:0019344 - cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-synthase"
                     /protein_id="HSF26000.1"
                     /translation="MKYYDSVVDLVGNTPLVRLTKVTEGISATVLAKVEYFNPGGSVK
                     DRIAVRMIDAAERDGLLKPGGTIVEPTSGNTGVGLALVAQQRGYRCVFVCPDKVSQDK
                     RSVLQAYGAEVVVCPTAVEPEHPDSYYSVSDRLVREIEGAWKPDQYSNPENPRSHYYS
                     TGPEIWADTDGKVTHVVIGVGTGGTISGTGGYLKEVSDGRVQVIGADPEGSVYSGGTG
                     RPYLVEGVGEDFWPTAYDQKVADEIVGVSDKDAFEMTRRLAREEGLLVGGSCGMAVVA
                     ALEVARRLGPDDVVVVLLPDGGRGYLSKIFNDDWMSSYGFLASDAE"
     gene            1091..2167
                     /locus_tag="VLC50_00635"
     CDS             1091..2167
                     /locus_tag="VLC50_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016910704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HSF26001.1"
                     /translation="MASRDRSGGRARAARRIAVKAAYGAAWGGGGAGLVGFGAVGLLA
                     TQAKLARRRIPSADGLPPNADGRYEGRHRGGTPIRMAMLGDSSAAGYGCEHAWQTPGA
                     VIARGLAKAARRPVELTCVAVVGARSTDLDAQVAAVLDRAPDVAVVMIGANDVTHRVR
                     PSTSVRALDQAIRRLREIDCEVVVGTCPDLGTLEPVAQPLRLIARRTSRRLAAAQTIV
                     TVEAGGRSVSLGDLLGPEFAARPGELFGPDRFHPSPVGYRSAALALLPSVCAALDVWT
                     EAVPAETRPDAARGEGVRPVAVAAAEAAETAGTEVAGAQVAGDDRGPRGRWALLRRRR
                     RAEPVDDVERTHQAPAEATPAGRD"
     gene            2210..3457
                     /locus_tag="VLC50_00640"
     CDS             2210..3457
                     /locus_tag="VLC50_00640"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005466189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HSF26002.1"
                     /translation="MPEAVIVATARSPIGRAYKGSLKELRPDDLTATIVRAALDKVPG
                     LDPRDIDDLMLGCGLPGGEQGFNMARVVAILLGYDHLPGVTLTRYCSSSLQTTRMAFH
                     AIKAGEGDVFISAGVEMVSRYARGNSDALPAEAQQAVGGSWHNPAFADARARSAERAA
                     GGADVWDDPRAAGTLPDIYLAMGQTAENVAALRGITREMQDEFGVRSQNLAEKAIADG
                     FWAREITPVTTPDGTVVAQDDGPRPGVTLEGVAGLSPVFRPDGTVTAGNCCPLNDGAA
                     AVIVMSDTKARELGLTPLARIISTGVSALSPEIMGLGPVDATRQALARAGMTIEDIEL
                     AEINEAFAAQVIPSYQELGIPLERLNVNGGAIAVGHPFGMTGARITGTLLNSLEWHDK
                     TIGLETMCVGGGQGMAMIVERLS"
     gene            complement(3694..4113)
                     /locus_tag="VLC50_00645"
     CDS             complement(3694..4113)
                     /locus_tag="VLC50_00645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF26003.1"
                     /translation="MALTSTDPASGEDARAGLERELRRTCDRVATLTLARLTAAAPAA
                     DSPADRVRETAQRLVEVAAGIEAREAPSPPVPRGLPTLRAHALADQLAVTGTDVLLVA
                     AGLPDEASVWDHAVRRPLAEALAGALEAVVALRRALP"
     gene            4210..4440
                     /locus_tag="VLC50_00650"
     CDS             4210..4440
                     /locus_tag="VLC50_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003975729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4287 domain-containing protein"
                     /protein_id="HSF26004.1"
                     /translation="MSLHHSEETHQQLVARIPVATGRELPEWFQALEKGPGLVRFDER
                     VNWLRDEHNLSHGHATAIVHEHDRLRAQRKIS"
     gene            4443..4853
                     /locus_tag="VLC50_00655"
     CDS             4443..4853
                     /locus_tag="VLC50_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020576145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HSF26005.1"
                     /translation="MELEQAQELLRHHHRAVMATFRADGRPQLSPVLATLDAAGRVVV
                     STREPSMKVRNLRRDPRISLCLLNPGFFGDWAQVEGVAEIVGQPDAMEPLIDYYRSIS
                     GEHPDWGDYRAAMVREQRVVVRFEITRAGPTVAG"
     gene            complement(4870..5685)
                     /locus_tag="VLC50_00660"
     CDS             complement(4870..5685)
                     /locus_tag="VLC50_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020576144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HSF26006.1"
                     /translation="MESNNPVFRRSGAFSSRGYATFAPAPSAASLQEMYDAPSATAAQ
                     MGRMTLDDVIAKTGLMFVVLLVGAAASWFVLTPAMPAVPFLAMIAGLVLGMVIAFKQS
                     TSPGLILTYAAVEGVFVGGISLFYADYAASRSDGVNIVAQAVLGTLAAFALMLVLYRS
                     GKLRATPKFTKMLLVAGGAYLLIALASFVAAMFGVGDGWGFRTGGLGLLLCAAGVALA
                     SFFLILDFDFIEQGIRNGAPERTAWLAGFGLMVTLIWLYLEILRLLAILRGGE"
     gene            5972..6109
                     /locus_tag="VLC50_00665"
     CDS             5972..6109
                     /locus_tag="VLC50_00665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF26007.1"
                     /translation="MSGWGFGYPGHPSDEVLKAKEEAEAKPAEQNDADNATDGSDAPS
                     A"
     gene            complement(6183..7202)
                     /locus_tag="VLC50_00670"
     CDS             complement(6183..7202)
                     /locus_tag="VLC50_00670"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HSF26008.1"
                     /translation="MSKRATIAEVAELASVHKATVARALNPDTAHRVSAETAKRIQCA
                     AEQLGYIPNIVARGLRKSLSMTIGVVVRDLENPIFPPIVRGIESHLSARGYTALIANT
                     DCCDVLERAAFDSLLQRRVDGFVMATGLADHPLLAQAFERGIRVVVVNPGIDAVPCPL
                     VTGADADGMAAAVEHLLGLGHRHVLHMAGPDIFTTSQVRSAAFTAALGRRRGVTSRVV
                     PAQGESVAAGRATMDMVLASGQPCPTAIVAGNDRLAIGILHSLRAHGLDCPRDVSVIG
                     FDDMPFAEDIQPPLTTVRVPHRELGVEAARVLLRAIQGGEQEPRVVLPVSLIVRGSTA
                     PPVAR"
     gene            complement(7358..8971)
                     /locus_tag="VLC50_00675"
     CDS             complement(7358..8971)
                     /locus_tag="VLC50_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009359507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HSF26009.1"
                     /translation="MKSYDVPVLVVGGSLVGMTTAMLLAKQGVKSLVVEKHPGSAIHP
                     RAALMLQRSMEIFRSAGVEDEMRTRSFNQFEPDGAIMAVETMAGKEIAWYMTRVNDGV
                     RDLSPSERIFVTQIAVEPVLKGRAEELGAEVLFGTELVSVTEDADGVSAVIRDRTTSE
                     ESNVRAKYLIAADGPHSTVREQLGITRSGHGVLSKSITIYFRADIEPLLKGRNLSVIM
                     VVNDVLQGFFRFEKPYQSGFLVVHGLGDPQRPNSDLWTGLSDESCIEMVRAGLGVDDI
                     PVEINNVMQWEATAVTADEFRRGRTFLVGDAAHEMPPYGGYGGNTGIHDAHNLAWKLA
                     AVLNGSAGEDLLSSYDAERRPAAAFTVEQGYSRFVVRAAPYRAADGIEPVVPDANIDL
                     GYRYRSSAVIADANDDGAIQSDPRELHGLPGSRAPHYELTLGGKQVSTLDFFNRNFVL
                     FTGADAKAWRESGEAAGRELGLDLDLYTVDAVADPAGGFAESYGITSTGAVLVRPDGF
                     VAWRAETDAGADQHSLSTVLGTVLGRAVA"
     gene            complement(9016..9672)
                     /locus_tag="VLC50_00680"
     CDS             complement(9016..9672)
                     /locus_tag="VLC50_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013643734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HSF26010.1"
                     /translation="MQSIAARRAVISSSEPGVHMTNPILDPITADNSAVLLVDFQRAM
                     FAGIGSGDRDAIHTSVVATVKAARILDVPVVLSSIWEGGNGPFIGPITEQFPNEKVYE
                     RSEQSFDALENPEVSAAVRATGRKKLIVSGLWTSMCFAFTALHAAQDGYEVYGLIDAA
                     GDATPDAHFYGQQRMYQAGIVPITWMPLVSHWMHDWTNPKAGDLKAEVFGAYDAILAM
                     "
     gene            complement(9772..9845)
                     /locus_tag="VLC50_00685"
     tRNA            complement(9772..9845)
                     /locus_tag="VLC50_00685"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9809..9811),aa:Leu,seq:taa)
     gene            complement(9874..11163)
                     /locus_tag="VLC50_00690"
     CDS             complement(9874..11163)
                     /locus_tag="VLC50_00690"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003968670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HSF26011.1"
                     /translation="MPAADRLVQLFTQVLGEAPPLRLRAWDGSEAGPAEGPVLVVRDR
                     RAVRRLVWRPDEVGLGRGYVAGELDVEGDLYEVLTRLAFITEHDDRPDHPLSVADKAR
                     VAATAIRLGAVGPEPTPPPEEAGSRAGARHTRSRDQAAISHHYDVGNEFYRHVLGPSM
                     VYSCAYWTRAGDASYSLADAQRDKLELVSRKLGLRPGMRLLDVGCGWGSMALHAAAEH
                     GAQVVGITVSRAQAEMARKRIAEAGLTERVEIRLQDYRDVDDGPYDAISSIGMAEHVG
                     VGPYLTYARHLFDLLRPGGRLLNHQIARRPGPSDPDHESFISAYVFPDGELAPLGTTV
                     SLIEEAGFEVRDVQALREHYALTLREWVRNLEEHWDEAVRLTSPGRARVWRLYMAGSA
                     LAFERNRIGVNHVLAVKPQSGGSSTLPLRRTGWLDLD"
     gene            complement(11255..12532)
                     /locus_tag="VLC50_00695"
     CDS             complement(11255..12532)
                     /locus_tag="VLC50_00695"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020576139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HSF26012.1"
                     /translation="MYAALRLQRRLRRGEADVVVIDPRSYMTYQPFLPEAAAGNLEPR
                     HVVVPLRRVLDRTHVLNAEVTAVDPHAKVVSVRPAGGVVEAMRYDVLVLAAGSVARTL
                     PIPGLAEVGIGFKHVEEAIALRNTVLDQLDQAAATTDAARRRCALTFVFVGGGYAGVE
                     ALAELEDMARYATRFYPDLEPGDLRFVLVEAAGRILPEVGEDLGRYTVAELRRRGIEV
                     RLGTRLESCVDGHVRLSDGDEFDSDVIVWTAGVKANPVVARSGLPLDAKGRIACRADL
                     RVEGFDDVWSAGDAAAVPDLTSPEPGATCGPSAQHAVRQGKLLGDNIAAVLHGGVPAV
                     YRHSYAGSVASLGLHKGVAQVYGVKVRGWPAWFMHRTYHVSRMPTLNRKARVVADWTL
                     ALFFRREVVALGSLRHPRRRFEEAAARTPRVSS"
     gene            complement(12660..13478)
                     /locus_tag="VLC50_00700"
     CDS             complement(12660..13478)
                     /locus_tag="VLC50_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019873244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HSF26013.1"
                     /translation="MGPAVQPEIRGPVPPGSGWPEDPAGPATPVAHDPAQVLKLAASA
                     DDLAELEARQSVCRACPRLVEWREQVAVVRRRQWADEQYWGRPIPGWGDRDARILIVG
                     LAPAAHGGNRTGRIFTGDRSGDWLFAALHRAGLARLPTSQHAGDGQALIGARLLSAVR
                     CAPPDNKPTPAERDACAPWLDRELALLLGQLRVIVVLGAFGWAATMTALTRAGLPVPR
                     PRPKFGHGATVALGDLLVIGSFHPSQQNTFTGRLTEAMLDEVLARACEHAAASS"
     gene            complement(13583..15055)
                     /locus_tag="VLC50_00705"
     CDS             complement(13583..15055)
                     /locus_tag="VLC50_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014740803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSF26014.1"
                     /translation="MISRPAAVAASAIVLPLALAQFVASYAGTNMNVAISDIADDLDT
                     TVLALQTTITLFTLTMASLMIPGSKLTDIWGRKRCFVLGLFIYGAGALLALFAQGITL
                     MIIGYSLLEGIGSALMIPPIYILITVAFTDTHTRAKYFGVVSAAAGIGAAAGPLIGGL
                     ITSAISWRASFLLQVLIVAAIIVLSRRIVEPPRGAAPPFDLLGAVLSAAGLLFVVLGV
                     LQASTYGWLSSREDFTIGDVVLIPEGGISPVWIFIGIGALILLWFFLHLRATERAGRT
                     PLIATRLFRNRKSNLGLLTQNVQWLTMQGTFFVLSVFLQQVRGYTAIQTGLMLTPTTV
                     GILLASAAAGRMAQRRRQKGLVSGGFVATTLGMLLLLALAREDSSVLSYVPGLLLIGL
                     GVGIMLTASVNVVQSSFPDSDQGEISGLSRSVSNLGSSLGVALAGSILVAANVPGGKP
                     FAISLVILTAISLIGLLAALLLPHEASPRSRQAVPEQREA"
     gene            complement(15159..>15560)
                     /locus_tag="VLC50_00710"
     CDS             complement(15159..>15560)
                     /locus_tag="VLC50_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018566940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HSF26015.1"
                     /translation="DPPTPAEIAAASAAIDAAIEVAGQAVPFAAARTLVGLAGSVTTV
                     AAVVLDLPAYDASRIHLSRLPAAAVRDAVRRLLTATHAERAALPFMHPGRVDVIGGGA
                     LVLDRVLAATGVDELVVSEHDILDGIAFSIA"
BASE COUNT         2388 a         5492 c         5548 g         2132 t
ORIGIN      
        1 tctcggcgtc cgaggccagg aagccgtacg aggacatcca gtcgtcgttg aagatcttcg
       61 agaggtagcc gcggccgccg tcgggcagca gcaccaccac cacgtcgtcg ggacccagcc
      121 ggcgcgcaac ctccagcgcg gcgaccacgg ccatgccgca ggagccgcca accagcaggc
      181 cctcctcgcg ggccagccgg cgggtcatct cgaacgcgtc cttgtcgctg accccgacga
      241 tctcgtcggc caccttctgg tcataggcgg tcggccagaa gtcctcgccg acgccctcga
      301 ccaggtaggg ccgcccggtg ccgccggagt agaccgagcc ctccgggtcg gccccgatca
      361 cctgcacccg gccgtcgctg acctccttga ggtagccgcc ggtgccgctg atggtgccgc
      421 cggtgccgac gccgatcacc acgtgggtga ccttgccgtc ggtgtcggcc cagatctccg
      481 ggccggtgga gtagtagtgg ctgcgcgggt tctcggggtt ggagtactgg tcgggcttcc
      541 acgcgccctc gatctcgcgg accagccggt cagagaccga gtaatagctg tcggggtgct
      601 ccggctccac cgcggtgggg cagaccacca cctcggcgcc gtacgcctgc agcacgctgc
      661 gcttgtcttg gctgaccttg tcggggcaga cgaacacgca ccggtagccg cgctgctggg
      721 ccaccagggc cagccccacc ccggtgttgc ccgaggtcgg ctccacgatg gtgccgcccg
      781 gcttgagcag cccgtcgcgc tcggcggcat caatcatgcg tacggcgatg cggtccttca
      841 ccgacccgcc ggggttgaag tactccacct tggccagcac ggtggccgag atgccctcgg
      901 tgaccttcgt cagacggacc agtggggtgt tgccgacgag gtcgaccacc gagtcgtagt
      961 acttcacgag gcgcaagcct agagggccga ccgcgagtcg cagccggcca ccgtcgtcgc
     1021 cgccgcgggt tagcctgcga gcactgcacc tggcacagcg aagcaacgcg actggcgagg
     1081 gaggcggacg gtggcgtctc gggatcgctc cggtggtcgg gcccgtgcgg cccggcggat
     1141 cgcagtcaag gcggcgtacg gcgcggcctg gggcggcggg ggcgcggggc tggtggggtt
     1201 cggcgccgtg gggctgctgg ccacccaggc caagctcgcc cgccggcgca tcccgtccgc
     1261 cgacggactc cctcccaacg ccgacggccg ctacgagggt cgacaccgcg gcggcacccc
     1321 gatccggatg gccatgctcg gcgactccag cgccgccggc tacggctgcg agcacgcctg
     1381 gcagaccccg ggtgcggtga tcgcccgcgg actggccaag gcggcccgcc gtccggtcga
     1441 actgacctgc gtggccgtgg tcggggcccg ctcgaccgac ctggacgccc aggtcgccgc
     1501 ggtgctcgac cgggcgcccg acgtggccgt ggtgatgatc ggcgccaacg acgtcaccca
     1561 ccgggtgcgc ccgtcgacct cggtgcgcgc gctcgaccag gccatccgcc ggctgcgcga
     1621 gatcgactgc gaggtggtcg tgggcacctg cccagatctg ggcaccctgg agccggtcgc
     1681 gcagccgctg cggctgatcg cgcgccggac cagccggcgg ctggccgcgg cccagacgat
     1741 cgtcaccgtc gaggctggcg ggcgcagcgt ctcgctcgga gacctgctcg gacccgagtt
     1801 cgcggcccgg ccgggtgagc tgttcggccc cgaccggttc cacccttccc cggtcggcta
     1861 tcgcagcgcg gcactggcgc tgttgccgtc ggtgtgcgcg gcgctcgacg tctggaccga
     1921 ggccgtgccc gccgagaccc ggcccgacgc cgcccgcggc gagggcgtgc gcccggtcgc
     1981 ggtggccgcc gccgaggccg ccgagaccgc cggcaccgag gtcgccggcg cccaggtggc
     2041 cggcgacgac cgaggcccgc ggggccgctg ggcgctgcta cgccggcgcc ggcgggccga
     2101 gccggtcgac gatgtggagc gcacgcacca ggcgccggcg gaggccaccc cagccggccg
     2161 cgactaggct cgccgggacg ccgtcgtacg actcacgggg agactcacca tgcctgaggc
     2221 cgtcatcgtc gcgaccgccc gctcaccgat cgggcgggcc tacaaggggt cgctcaagga
     2281 gctgcgcccc gacgacctga cggccaccat cgtgcgcgcc gccctcgaca aggtgcccgg
     2341 gctggacccg cgcgacatcg acgacctcat gctcggctgc gggctgcccg gcggcgagca
     2401 gggcttcaac atggcccggg tggtggccat cctgctcggc tatgaccacc tgcccggggt
     2461 cacgctgacc cgctactgct cctcgtcgct gcagaccacc cggatggcct tccacgcgat
     2521 caaggccggc gagggcgacg tgttcatctc ggccggcgtc gagatggtca gccgctacgc
     2581 ccgcggcaac agcgacgcgc tgccagccga ggcgcagcag gcagtcggcg ggtcgtggca
     2641 caacccggcc ttcgccgacg cgcgagcccg cagcgccgag cgcgccgccg gcggggcaga
     2701 tgtctgggac gacccccggg ccgccggcac gctgcccgac atctacctcg cgatggggca
     2761 gaccgccgag aacgtcgcgg cactgcgcgg catcacccgc gagatgcagg acgagttcgg
     2821 cgtgcgctcg caaaacctgg ccgagaaggc aatcgccgac gggttctggg cccgcgagat
     2881 caccccggtg accacccccg acggcaccgt ggtcgcgcag gacgacggcc cacgaccggg
     2941 cgtgacactc gagggcgtgg ctgggctctc gccggtgttc cggcccgacg gcaccgtcac
     3001 cgccggcaac tgctgcccgc tcaacgacgg cgcggccgcg gtcatcgtga tgagcgacac
     3061 caaggcccgc gagctcggcc tgaccccgct ggcccggatc atctccaccg gggtcagcgc
     3121 gctctcgccc gagatcatgg ggctgggtcc ggtcgacgcg acccggcagg cgctggcccg
     3181 ggccggcatg acgatcgagg acatcgagct cgccgagatc aacgaggcgt tcgccgccca
     3241 ggtgatcccg agctaccagg agctgggcat cccgctcgag cggctcaacg tcaacggcgg
     3301 cgcgatcgcg gtcggccacc cgtttggcat gaccggggcc cgcattaccg gcaccctgct
     3361 caactcgctc gagtggcacg acaagacgat cgggctcgag accatgtgcg tcggcggcgg
     3421 ccagggcatg gccatgatcg tcgaacgcct ctcgtgaccc ccgtcgcgat cttcgcgatc
     3481 acctctcgcc gcccagccct cgcccgccac atcctcgggg ccacaacctc gttgcgcccg
     3541 aaccgcacct tgctcccgtg ttggcaccac aagaggggca tttcgggcgg gagaacgtgc
     3601 ggatcgggcg caacgtcgtc gtggtgcaag gcgcccagct ccgtgatcag ggcgggagcg
     3661 gggcgggagg tctgagcgga gctggtcggc acgtcagggc agcgcgcggc gtagggccac
     3721 cacggcctcg agcgccccag cgagcgcctc ggccagcggt cgccgtacgg cgtgatccca
     3781 gaccgacgcc tcgtcgggca gcccggcggc caccaggagt acgtcggtgc cggtgaccgc
     3841 cagctggtcg gccagcgcgt gcgcccgcag ggtcggcagc ccccggggca ccggcgggct
     3901 cggcgcctct cgcgcctcga tcccggcggc gacctcgacc agccgctggg cggtctcccg
     3961 gacccggtcg gccgggctgt cggcggccgg cgccgcggcg gtcagccggg ccagggtcag
     4021 ggtcgcgacc cggtcacagg tgcggcgcag ctcgcgctcc agccccgcgc gggcgtcctc
     4081 gccggacgcg ggatcagtgc tggtcagggc cacgtcggga ccttatgccc gaagccgcgc
     4141 gagggggttg tgcctgcgag ggggctcagg cacgctcgac tcacgacacc ctcgtccagg
     4201 gaggcgcaca tgtccctgca ccactccgag gagacgcacc agcagctggt cgcccgaatc
     4261 ccggtagcaa ccggacgtga gctccccgag tggttccagg cgctggagaa gggtcccggt
     4321 ctggtgcgct tcgacgaacg ggtcaactgg ctgcgcgacg agcacaacct cagccacggg
     4381 cacgccaccg cgatcgtgca cgagcacgac cggctgcgtg ctcagcgtaa gatctcttga
     4441 ccgtggagct tgagcaagcg caggagctcc tgcgccacca tcaccgtgcg gtgatggcca
     4501 ccttccgcgc cgacgggcgc ccccagctct cccccgtgct cgccaccctc gacgcggccg
     4561 gtcgggtcgt ggtgtccacc cgcgagccgt cgatgaaagt gcgcaacctg cgccgggacc
     4621 cgcggatctc gctgtgcctg ctcaaccccg ggttcttcgg cgactgggcc caggtcgagg
     4681 gcgtcgccga gatcgtgggc cagcccgacg cgatggagcc gctcatcgac tactaccgct
     4741 cgatctccgg cgagcacccc gactggggcg actaccgggc cgcgatggtg cgcgagcagc
     4801 gggtcgtcgt acggttcgag atcactcggg ccggcccgac cgtcgccggc tgagccccca
     4861 cagcggcggc tactcgccgc cgcgcaggat ggccagcagg cgcaggatct cgaggtagag
     4921 ccagatcagc gtgaccatca gcccgaagcc ggccagccac gccgtgcgct cgggggcgcc
     4981 gttgcggatc ccctgctcga tgaagtcgaa gtcgaggatg aggaagaagc tggccagcgc
     5041 cacgcccgcg gcgcacagca gcagcccgag cccgccggtg cggaagcccc agccgtcgcc
     5101 gacgccgaac atcgcggcga cgaagctcgc cagcgcgatg agcaggtagg ccccgccggc
     5161 gaccagcagc atcttggtga acttcggggt cgcccgcagc ttgccgctgc ggtagagcac
     5221 gagcatcaga gcgaacgccg ccagggtgcc gagcaccgcc tgggcgacga tgttgacgcc
     5281 gtcgctgcgg gaggcggcgt agtcggcgta gaagaggctg atgccgccga cgaagacgcc
     5341 ctcgaccgcg gcgtaggtga ggatcaatcc ggggctggtg gactgcttga acgcgatgac
     5401 catgccgagc accaggccgg cgatcatcgc caggaacggc acggccggca tcgccggggt
     5461 caggacgaac cagctggccg ccgcacccac gaggagcacg acgaacatca ggccggtctt
     5521 ggcgatgacg tcgtcgaggg tcatccgacc catctgggcg gcggtcgccg acggggcgtc
     5581 gtacatctcc tgcagagacg ccgcgctggg cgcgggcgcg aaggtcgcgt aaccgcggct
     5641 cgagaaggcc ccgctgcggc ggaagaccgg gttgttgctc tccatctttg gcctccggga
     5701 tgccccgtgc cggggcgacg gtggtcgtcg tcgtacgacg caacgtggcc caccctaacg
     5761 tgtgctcccg tgaaggcgcc tttggcgagc gtgacggtgg cgcgcgagcc ggcgccgggg
     5821 tcgcgaacgt ttccatgact gcattcgatt gccgtgagcc tagacgcagc gaacacagag
     5881 cgttagcctt gaaaacacaa cgcctctcac gttttacggg cggaagcgtt tgcgaagttc
     5941 atcgggatga ccacggcacg gaggttggac agtgagcgga tggggtttcg gttacccggg
     6001 ccacccttcg gacgaggtgc tcaaggccaa ggaagaggcc gaagccaagc ctgccgagca
     6061 gaacgacgcc gacaacgcga ccgacgggtc agacgcgccg agcgcctgag ctcggctcat
     6121 cgcctccgtt cgggccgaaa caatcgactg cgacaactga ccggcgaggt gggcgcctcg
     6181 cctcagcgcg cgaccggcgg cgccgtcgac ccgcgcacga tgagcgagac cgggaggacc
     6241 accctgggct cctgctcgcc tccttggatg gcgcgcagga gcacccgggc cgcctcgaca
     6301 cccagctcgc ggtgcggcac gcggaccgtc gtgagcggcg gctggatgtc ctcggcaaac
     6361 ggcatgtcgt cgaacccgat gacggacacg tcgcgcgggc agtccaggcc atgcgcgcgc
     6421 agcgagtgca ggatcccgat ggcgagacga tcgttgccgg ccacgatcgc ggtggggcac
     6481 ggttgcccgc tggccaggac catgtccatg gtcgctcgcc cggcggcgac gctctccccc
     6541 tgagcgggga ccactcggct ggtcacgccg cgccgacgac cgagcgcggc agtgaaggcg
     6601 gctgaccgga cttggctggt ggtgaagatg tccgggccgg ccatgtgcag cacgtgccga
     6661 tgcccgagcc ccagcaggtg ctcgaccgcg gcggccatgc cgtcggcgtc ggcgcccgtg
     6721 accagcgggc agggcacggc gtcgatcccc gggttgacca cgaccaccct gatgccgcgc
     6781 tcgaacgcct gcgcgagcag cggatggtcg gccaggccgg tggccatgac gaaaccgtcg
     6841 acgcgacgtt gcaacagcga gtcgaaggcg gcacgttcca gcacgtcgca gcaatcggtg
     6901 ttggcgatca acgcggtgta gccgcgcgct gagaggtggc tttcgatgcc ccgcacgata
     6961 ggcggaaaga tcgggttctc aaggtccctg accacgacgc cgatcgtcat cgacaggctt
     7021 ttgcgcagcc ctcgagccac gatgttgggg atgtagccga gctgttcggc cgcgcactgg
     7081 atccttttgg ccgtctcggc gctgacacga tgcgcagtgt cggggttgag cgcgcgggcc
     7141 accgtcgcct tgtgcacgct cgcgagctcg gccacctcgg cgatcgtcgc gcgcttggac
     7201 acgggtggag gctaccgccc tgttgccggc tcaccgccga gacacccgca aaaggaagac
     7261 ctccgggtga gagcgtggaa tctgagcttc ccacgcctca ctcggaggtc ttctcagtca
     7321 ggacgccagt ttcggcgcaa cgactacctc agggcagcta ggccacggca cgcccgagca
     7381 cggtcccgag aaccgtcgag agggaatgct ggtcggctcc cgcatcggtc tcggctcgcc
     7441 aggcgacgaa gccgtcgggg cgcaccagca ccgcgccggt cgaggtgatc ccgtagctct
     7501 ccgcaaaccc gcctgccggg tcggcgacgg catcgaccgt gtagaggtcg agatccaggc
     7561 cgagctcgcg gcctgccgcc tcgcccgact cccgccacgc cttggcatcg gctccggtga
     7621 acagcacgaa gttgcggttg aagaagtcga gcgtcgagac ctgcttgccg cccagggtga
     7681 gctcgtagtg cggcgcccgc gacccaggca gaccgtggag ctcgcgcggg tcactctgga
     7741 tggctccgtc gtcattcgcg tccgcaatca ccgccgacga ccggtagcgg tagcccaggt
     7801 cgatgttggc gtcgggaacc accggctcga tcccgtcggc cgcccggtag ggagcggccc
     7861 gcaccacgaa tcgtgaatat ccctgctcca ccgtgaacgc ggcggcgggc cgacgctcgg
     7921 cgtcatagct cgacaggagg tcctcgcccg cgctcccgtt gagcacggca gcgagcttcc
     7981 aggcgaggtt gtgagcgtcg tggatgccgg tgttgccgcc ataaccgccg tagggcggca
     8041 tctcgtgggc ggcgtctccg acgaggaagg tgcgtccccg tcggaactcg tcggccgtga
     8101 cggcggtggc ctcccactgc atgacgttgt tgatctcgac cgggatgtcg tcgaccccga
     8161 gcccggcacg caccatctcg atgcacgact catcgctgag cccggtccac aggtcgctgt
     8221 tgggacgctg cggatcgccc aggccgtgga cgaccaggaa tcccgactgg tagggcttct
     8281 cgaagcggaa aaagccctgg agcacgtcgt tgaccaccat gatgacgctg aggttgcgcc
     8341 ccttgagcag cggctcgatg tcagcacgga agtagatcgt gatgctcttg gagagcaccc
     8401 cgtggccgct gcgcgtgatg cccagctgct cgcggaccgt gctgtgcggc ccgtcagcgg
     8461 cgatgaggta tttggcccgc acgttggact cttccgaggt ggtgcgatcg cgaatgaccg
     8521 cgctcacgcc gtcggcgtcc tcggtgacgc tgaccagctc ggtgccgaac agcacctcgg
     8581 cgcccagctc ctccgcacgg cccttgagca ccggctcgac ggcgatctgg gtgacgaaga
     8641 tgcgttcgga cggactcagg tcacgcacgc cgtcgttgac ccgagtcatg taccaggcga
     8701 tctccttgcc ggccatcgtc tcgaccgcca tgatcgcgcc atcgggctcg aactggttga
     8761 acgacctcgt gcgcatctcg tcttcgacgc cggcgctgcg gaagatctcc atcgagcgct
     8821 gcagcatgag tgccgcacgc gggtggatcg cggatccggg atgcttctcg actacgaggc
     8881 tcttcacgcc ctgcttggcc agcagcattg ccgtggtcat tccgaccagg ctgcccccga
     8941 ccacaagaac gggaacgtca taactcttca tgatcctcaa ccacctcctc gcgtgtcagg
     9001 aatcagctgc tgggctcaca tcgccaagat cgcgtcgtag gcaccgaaga cctctgcctt
     9061 gaggtcgcca gccttggggt tggtccagtc gtgcatccag tgagagacca gcggcatcca
     9121 ggtgatcggc acgatgccgg cctggtacat ccgctgctgg ccgtagaagt gggcgtcggg
     9181 cgtggcgtcg ccggctgcat cgatgaggcc gtagacctcg tagccgtcct gggcggcatg
     9241 cagcgcggtg aaggcgaagc acatgctggt ccacagcccg gagacgatca gcttcttgcg
     9301 gccggttgcc cgcaccgcag ccgaaacctc ggggttctcg agagcgtcaa agctctgctc
     9361 cgaacgctcg tagaccttct cgttggggaa ctgctcggtg atcgggccga tgaacggacc
     9421 gttgccgcct tcccagatcg agctcagcac gaccgggacg tcgagaatcc gtgcagcctt
     9481 caccgtcgcc acaacgctcg tgtgaatcgc gtcgcggtcg ccagagccga ttcccgcgaa
     9541 catcgcacgt tgaaagtcga ccagcaggac cgcgctgttg tcagcggtga tggggtcgag
     9601 gattgggttg gtcatgtgaa ctccaggctc cgacgacgaa atcacagccc tcctggctgc
     9661 gatcgattgc atcgtgcccc tggccaaccc gcctggtcaa gagtcaacga gcgcggattt
     9721 atgaggagat aagcccggaa aaacaaacgc ccaggtgaac ggcctgatcg gtgcccccgg
     9781 tgggggtcga acccacactg ggcggtgttt aagaccgctg cctctgccga ttgggctacg
     9841 ggggcgcgag cctgacgcta ccgaacgggc ggttcagtcg aggtcgagcc acccggtacg
     9901 acgcagcggc agcgtggagc tgcccccgga ctggggcttg accgccagca cgtggttgac
     9961 cccgatccgg ttgcgctcga acgcgagcgc ggagccggcc atgtagagcc gccagacccg
    10021 ggcccggccg ggcgaggtga gccgcaccgc ctcgtcccag tgctcttcga ggttgcgcac
    10081 ccactcgcgc agcgtcaggg cgtagtgctc acgcagcgcc tgcacgtcgc gcacctcgaa
    10141 gccggcttct tcgatgagcg acacggtggt gcccagcggg gcgagctcgc cgtcgggaaa
    10201 gacgtaggca gagatgaacg actcgtggtc ggggtcggag gggccggggc ggcgggcgat
    10261 ctggtggttg agcagccgcc cgccggggcg cagcaggtcg aacaggtgac gggcataggt
    10321 caggtagggg ccgaccccga cgtgctcggc catgccgatg ctcgagatgg cgtcgtaggg
    10381 cccgtcgtcg acgtcgcggt agtcctgcag ccggatctcc acccgctcgg tcaggccggc
    10441 ctcggcgatc cgcttgcgcg ccatctcggc ctgcgctcgg gagacggtga tgccgaccac
    10501 ctgggcgccg tgctcggcgg cggcgtgcag cgccatagac ccccagccac agccgacgtc
    10561 gagcagacgc atgcctggac gcagaccaag cttgcggctg accagctcga gcttgtcgcg
    10621 ctgggcatcg gccagcgagt aggacgcatc gccggcccgg gtccagtagg cgcacgagta
    10681 gaccatcgac gggcccagga cgtgccggta gaactcgttg cccacatcgt agtgatgact
    10741 gatcgcggcc tggtcgcggc tgcgggtgtg ccgagccccg gcgcgcgagc cggcctcttc
    10801 cggcggaggg gtgggctcgg ggccgaccgc gccgagccgg atcgccgtgg cggccacccg
    10861 agccttgtcc gccaccgaga gcggatggtc gggcctgtcg tcgtgctcgg tgatgaaggc
    10921 cagccgggtc agcacctcgt agagatcacc ttcgacgtcg agctcaccgg cgacgtagcc
    10981 gcgacccagc cctacctcgt cggggcgcca gaccagtcgt cgtacggcgc ggcggtcgcg
    11041 caccacgagc accggcccct cggccggtcc ggcctccgac ccgtcccagg cccgcagccg
    11101 cagcggcggg gcctccccga ggacctgggt gaacagctgg acgaggcggt cagcggcggg
    11161 cacgaacgag ctcccctcgg gtgacggcgg gtgactacca ggggtgacgc tatccagacc
    11221 gcaggcattc ccgcgggcgc gtgtcgggat ccggtcacga cgacacgcgc ggcgtgcgcg
    11281 cggcggcctc ctcgaaccgc cggcgcgggt ggcgcaagga gccgagggcg accacctcgc
    11341 ggcggaagaa caacgccagc gtccagtctg cgaccacgcg ggccttgcgg ttgagcgtcg
    11401 gcatccggct gacgtgatag gtgcggtgca tgaaccaggc tggccagcca cgcaccttga
    11461 cgccgtagac ctgcgcgacc cccttgtgca gcccgagcga ggccaccgac ccggcgtagg
    11521 agtgccggta gacggccggt acgcccccgt gcagcaccgc ggcgatgttg tcgccgagca
    11581 gcttgccctg gcgtacggcg tgctgagcgc tcggcccgca cgtggcgccc ggctcgggcg
    11641 acgtcaggtc gggcaccgcc gcggcgtcgc cggccgacca cacgtcgtcg aacccctcga
    11701 cccgcaggtc ggcccggcag gcgatgcggc ccttggcgtc cagcggcagt ccgctgcggg
    11761 cgacgacggg gttggccttg acccccgccg tccacacgat gacgtcgctg tcgaactcgt
    11821 cgccgtcgga gagccggacg tgcccgtcga cgcacgactc gagccgggtg cccagccgca
    11881 cctcgatgcc gcgccggcgc agctcggcca ccgtgtagcg acccaggtcc tcaccgacct
    11941 cgggcaggat ccgcccggcc gcctcgacca gcacgaaccg caggtctccc ggctcgaggt
    12001 cgggatagaa ccgggtcgcg tagcgggcca tgtcctccag ctcggccagc gcctcgaccc
    12061 cggcgtagcc gccacccacg aacacgaacg tcagcgcgca ccggcgccgg gccgcgtcgg
    12121 tggtggccgc ggcctggtcg agctggtcga gcaccgtgtt gcgcagcgcg atggcctcct
    12181 cgacgtgctt gaagccgatg ccgacctcgg cgagcccggg gatcggcagc gtgcgcgcca
    12241 cggagcccgc ggccaggacc agcacgtcgt agcgcatcgc ctcgaccaca ccaccggccg
    12301 gccgcaccga gacgaccttc gcgtgcgggt cgacggcggt cacctcggcg ttgaggacgt
    12361 gggtgcggtc gagcacccgg cgcagcggta cgacgacatg gcggggctcg aggttgcccg
    12421 cggccgcctc gggcaggaac ggctggtagg tcatgtagga gcgcgggtcg atgaccacga
    12481 cgtcggcctc accccgccgc agccggcgct gcagccgcag ggcggcgtac atgccgacat
    12541 agccgccgcc gacgatgacg atcctgggcc ggccgccccg cccggtcagg tgctggcggc
    12601 tccacgtcag gttcgccacc tctgcaccat aaccacgccc gtgccggggc ggcggggctt
    12661 caggacgagg ccgcggcgtg ctcgcaggct cgggccagca cctcgtcgag catcgcctcg
    12721 gtcaggcgac cggtgaaggt gttctgctgg cttgggtgga acgagccgat gacgagcagg
    12781 tcgcccagcg ccacggtggc gccgtggccg aacttcggtc gtggccgcgg caccggcagc
    12841 ccggcccggg tcagcgcggt catcgtggcg gcccagccga aggcgccgag tacgacgatc
    12901 acgcgcagct ggccgaggag gagggccagc tcgcggtcga gccacggcgc gcaggcgtcg
    12961 cgctcggccg gggtcggctt gttgtcgggc ggcgcgcagc ggaccgcgct gagcaagcgg
    13021 gcgccgatga gcgcctgccc gtccccggcg tgctggctcg tgggcagccg ggccagcccg
    13081 gcccggtgca gcgcggcgaa gagccagtcg ccgctgcggt cgccggtgaa gatgcgcccg
    13141 gtgcggttgc cgccatgggc ggccggggcc agcccgacga tgaggatgcg ggcgtcgcgg
    13201 tctccccagc cggggatcgg ccggccccag tactgctcgt cggcccactg cctgcgtcgt
    13261 acgacggcca cctgctcgcg ccactcgacc agccgcgggc aggcccggca caccgactgg
    13321 cgtgcctcca gctcggcgag gtcgtcggca gaggcggcca gcttgagcac ctgcgccggg
    13381 tcgtgcgcca cgggggtggc ggggccggct gggtcctcgg gccagccgct gcccgggggc
    13441 accggcccgc ggatctcagg ttgcaccgcc gggcccatcg agcccggcac tgccgcggcg
    13501 ctgcccacgc gacctccgcc ccactgccga cgatcacgca agtgttctac gggcagcctc
    13561 gtctctcaac tcgcgtcgtc gctcaggcct cgcgctgctc tggcacggct tggcggcttc
    13621 ggggtgaggc ctcgtgcggc agcagcaagg cggccagcaa cccgatcaag gagatcgccg
    13681 tgaggatcac cagcgagatc gcgaacggct ttccgccagg gacattggca gcgacgagga
    13741 tcgagccggc gagcgccacc ccgagcgagg agcccaggtt ggagacgctg cgcgacagac
    13801 ccgagatctc accctggtcg gagtcgggga agctcgactg cacgacgttg accgaggcgg
    13861 tgagcatgat gccgaccccg agcccgatga ggagcagccc ggggacgtag ctgagcacgc
    13921 tggagtcctc ccgggccagg gccaggagaa gcagcatgcc gagcgtcgtg gcgacgaatc
    13981 ccccgctgac caagcccttc tgcctccggc gctgggccat ccggccggca gcggccgagg
    14041 ccagcaggat gcccacggtg gtgggcgtca gcatcagccc ggtctggatc gcggtgtagc
    14101 cacgcacctg ctgcaagaac accgagagca cgaagaaggt gccctgcatg gtcagccact
    14161 gcacgttctg ggtgagcaga ccgaggttgg acttgcggtt gcggaacagt cgggtggcta
    14221 tcagcggtgt ccgtcccgcc cgctcggtgg cccgaaggtg caggaagaac cacagcagga
    14281 tgagcgcgcc gatcccgatg aagatccaca ccggtgagat gccgccctcg ggaatcagca
    14341 ccacgtcacc gatcgtgaag tcctcgcggg aggagagcca gccgtaggtc gacgcctgga
    14401 gcacgccgag cacgacgaag agcagcccgg ccgccgagag caccgctccg agcagatcga
    14461 agggcggtgc ggccccacgc ggtggctcga cgatccgccg ggacagcacg atgatcgcgg
    14521 ccacgatcag cacctgcagc aggaacgacg cgcgccagct gatcgcgctc gtgatcaggc
    14581 cgccgatcaa cggcccggcc gccgccccga tcccggccgc ggcgctgacc acgccgaagt
    14641 acttggcgcg ggtatgggtg tcggtgaacg ccaccgtgat gaggatgtag atcggcggga
    14701 tcatcagcgc cgagccgatg ccctcgagca acgagtagcc gatgatcatc aaggtgatgc
    14761 cctgggcgaa cagcgccagc agtgcacccg ccccatagat gaacagcccg agcacgaagc
    14821 agcgcttgcg cccccagatg tcggtcagct tgctgcccgg gatcatcaac gacgccatgg
    14881 tcagcgtgaa cagggtgatc gtggtctgca gggcgaggac ggtcgtgtcc agatcgtcgg
    14941 caatgtcact gatcgccacg ttcatgttgg tgccggcata gctcgccacg aactgggcga
    15001 gtgccagcgg cagcacgatc gccgaggccg cgaccgcggc cggccgagag atcaccgcca
    15061 cccgatcagg gtgcgcgcga gccgacaggg cggccctcac ccccggcggg tgaggtcagg
    15121 gcggttgggc tcagcgcgat tcagtcgagc agctgaggtc aggcgatcga aaaggcgatc
    15181 ccatcgagaa tgtcgtgctc gctgaccacg agctcgtcga ccccggtggc ggccaggacc
    15241 cggtcgagca cgagcgcccc accgccgatc acgtcgaccc ggccggggtg catgaacggc
    15301 agcgcggccc gctcggcgtg ggtcgcggtg agcagccggc gtacggcgtc tcgcaccgcc
    15361 gcagccggaa gccgggacag gtggatgcgg ctcgcgtcgt aggccggcag gtcgagcacc
    15421 accgcggcca ccgtggtgac gctgccggcc agcccgacca gcgtgcgtgc cgcggcgaac
    15481 ggcacggcct gcccggccac ctctatcgcg gcgtcgatgg cggcgctggc ggcggcgatc
    15541 tcggccgggg tgggcgggtc
//
LOCUS       DATIBB010000021        10075 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_272022, whole genome shotgun sequence.
ACCESSION   DATIBB010000021 DATIBB010000000
VERSION     DATIBB010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 10075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10075
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_272022"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            245..451
                     /locus_tag="VLC50_00715"
     CDS             245..451
                     /locus_tag="VLC50_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018515354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like protein Pup"
                     /protein_id="HSF26016.1"
                     /translation="MATKDAGGQQRVTRREDTEETAPQETADLTERKEKLDDDIDSIL
                     DEIDEALEENSEEFVRGFVQKGGE"
     gene            520..801
                     /locus_tag="VLC50_00720"
     CDS             520..801
                     /locus_tag="VLC50_00720"
                     /inference="COORDINATES: protein motif:HMM:NF014941.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VII domain-containing protein"
                     /protein_id="HSF26017.1"
                     /translation="MEQRREDQAGRCPICLAGPAVHVDHDHETGAVRGLVCFQCNAAL
                     GQLRSPFVIRRAAAYLEGTLASPRRVATGVFALLPHTAIREALAPTADA"
     gene            860..1702
                     /gene="prcB"
                     /locus_tag="VLC50_00725"
     CDS             860..1702
                     /gene="prcB"
                     /locus_tag="VLC50_00725"
                     /EC_number="3.4.25.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012920844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005839 - proteasome core complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome subunit beta"
                     /protein_id="HSF26018.1"
                     /translation="MSFNPDRAGLPAAFLAAGSSSFTEFLGRYAPDLLPARRTLPAAG
                     MTIEAPHGTTIVAVSFPGGVVMAGDRRATMGNYIASRDIEKVFPADDYSVVGIAGTAG
                     IAVEVVRLLQVELEHYEKIEGAVLSLDGKANRLAQMIRGNLAMAMQGLAAVPVFAGFD
                     LETSEGRIFSYDVTGGRYEEHRFHSVGSGSMFARGALKKLYRDDLTAEDAALACIESL
                     YDAADDDSATGGPDLTRQIFPIVSVVTSEGYRRLPDEEVGDLVRLVVDRRMQDPGGPK
                     APLR"
     gene            1761..2528
                     /gene="prcA"
                     /locus_tag="VLC50_00730"
     CDS             1761..2528
                     /gene="prcA"
                     /locus_tag="VLC50_00730"
                     /EC_number="3.4.25.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014145877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005839 - proteasome core complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome subunit alpha"
                     /protein_id="HSF26019.1"
                     /translation="MTTPFYVSPEQLMKDRADYARKGIARGRSVVVVAYDAGILFVAE
                     NPSRALHKISEIYDRIALAAVGRYNEFENLRIAGVRYADLRGYSYDRRDVTARGLANA
                     FAQTLGTVFSGPGEKPFEVELVVAEVGESTAADQLYRLTYDGSVGDEHGWVAMGGQAE
                     TVGAALEEHYRDGLGLQEALALAVQALGRDANGAQRELTPDQLEVAVLDRHRPRRKFR
                     RLQGSLLERLLGEASPAVEAAAAAEIAEADDTDPPRR"
     gene            2565..3926
                     /gene="pafA"
                     /locus_tag="VLC50_00735"
     CDS             2565..3926
                     /gene="pafA"
                     /locus_tag="VLC50_00735"
                     /EC_number="6.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015607508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA];
                     GO_process: GO:0070490 - protein pupylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Pup--protein ligase"
                     /protein_id="HSF26020.1"
                     /translation="MDRRIFGLENEYGVTCTFRGQRRLSPDEVARYLFRRVVSWGRSS
                     NVFLRNGARLYLDVGSHPEYATPECDDVRQLVTHDKAGERILEGLLVDAERRLREEGI
                     AGDVYLFKNNTDSAGNSYGCHENYLVGRHGEFSRLADVLIPFLVTRQIICGAGKVLQT
                     PRGAAYCVSQRAEHIWEGVSSATTRSRPIINTRDEPHADAERYRRLHVIVGDSNMSET
                     TTMLKVGATDLVLRMIESGVILRDLTLENPIRSIREVSHDITGRRVVRLANGREASAL
                     DIQGEYLAKARDFAARRGIDEGIVKQVLDLWERTLHAIGTDDLDLVGREIDWVIKHRL
                     IERYRIKHGLSLASPRIAALDLAYHDIHRGRGVYYLLERAGKVERFTRDLDIFQAKSV
                     PPQTTRARLRGEFIRRAQEKRRDFTVDWVHLKLNDQAQRTVLCKDPFRSVDERVDRLI
                     QSM"
     gene            4051..5028
                     /locus_tag="VLC50_00740"
     CDS             4051..5028
                     /locus_tag="VLC50_00740"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HSF26021.1"
                     /translation="MRRTTMSVLASLTALALLSACGSGSSDATPSGSATPAATPTFAV
                     TSDGPAPAVNGKAGSAPTLGPTEGKPSDKLIVKELTPGKGRAIAKGDLVLTDFLVQKW
                     GEDKPIGSTYAEGAPTSFPIGMSQVIPAWEKLIGTKVGTRVEMVVPPAAGFGAAGNEQ
                     AGIKATDTLVFVFDVRGAYAADAKANGAPGERPAAGLPTVADGEKGPVITVPKGAKAP
                     TTISSTRLITGEGAEVKSGQTIVVQYTGALWDKGTVFDSSWERGAPASFVIGKGAVIP
                     GWDETLVGTHVGDRVMLVIPPDKAYRDQAQGAIPANSTLVFVVDVIDAL"
     gene            5076..5444
                     /locus_tag="VLC50_00745"
     CDS             5076..5444
                     /locus_tag="VLC50_00745"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015888813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HSF26022.1"
                     /translation="MPQLEKPEIDFPAGDAPTTLQVEDLVVGDGAEASAGATVLVHYV
                     GVDFASGEEFDASWNRGEPIEFPLRGLIKGWQEGIPGMKVGGRRKLVVPPDLAYGPAG
                     AGHRLSGQTLIFVIDLLAVR"
     gene            5538..6515
                     /locus_tag="VLC50_00750"
     CDS             5538..6515
                     /locus_tag="VLC50_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019877217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HSF26023.1"
                     /translation="MSSNVSRNKTERLLNLTIALLATRRFLSREEIRTMVPGYSERDE
                     AFERAFERDKDDLREMGIPIETGSNSALFDDEPGYRIRRDAYALPEVTFTGDEMAVLA
                     VAAQAWSHAALGAPAASALRKLKAYGVGLDDVVVAGLAPRVETREPAFARLWEAVRDA
                     RPVRFGYRRSGADTTEERTVEPWGIVSWRGRWYVVGHDRGREATRVFRLSRVRGEVTL
                     LGRPGQVVPPSDVDLHAAVVAVAAAPARGTAQVRLRRGAGGFLRRRATAVTAADAEWE
                     VLDVPYGDLRQLGEELASLGAAAVALGPAELRAEVCGRLRAAAGLEAAS"
     gene            6512..7486
                     /locus_tag="VLC50_00755"
     CDS             6512..7486
                     /locus_tag="VLC50_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014740631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HSF26024.1"
                     /translation="MIPATARLDRLLALVPYLLARPGVRVEDVAADFGISPERLVDDL
                     QLLFVCGLPGHLPDDLIDASFEGGVVHVTNADTIARPLRLAADEAVALLAGLRMLAAL
                     PGAVGHDALDRALVKLERAAGDAAAATERMTLSVEAEDEVVATARAGLDQGRALTLDY
                     YVSGRDETTRRTVDPMRLLVVDGRAYLEAWCRRAEAVRLFRADRVEHIAVLDEPAAVP
                     ASAQQRDLSLGLFQPTADDVVITLDLTRNARWVAEHYPCEDVEDLPGGGLRVRLRTPE
                     PRWVVRLVLRLGADGQIVDPPTLAAEVRGQALAALAAYEETSVNSAQD"
     gene            7549..7887
                     /locus_tag="VLC50_00760"
     CDS             7549..7887
                     /locus_tag="VLC50_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF26025.1"
                     /translation="MTTIKASCPSCGDVDLTPRQLRLVVATKPGMSYYSFSCTHCRDI
                     VQRPAGDDVVRILVSGGVRPEPWDVPAEVLEQHDGPALTYDDLLDLALALHRMDRLVL
                     ALAPSTGARA"
     gene            7930..8106
                     /locus_tag="VLC50_00765"
     CDS             7930..8106
                     /locus_tag="VLC50_00765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF26026.1"
                     /translation="MVWFLVWVALVAGALLVIGLLVRRLWRAVKALGAELATTTAQLE
                     EITARLESLPDLPR"
     gene            8184..8471
                     /gene="tatA"
                     /locus_tag="VLC50_00770"
     CDS             8184..8471
                     /gene="tatA"
                     /locus_tag="VLC50_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003802229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043953 - protein transport by the Tat
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HSF26027.1"
                     /translation="MRGLFEGWHLVILLVLILLLFGAKRLPDAARGLGRSMRIFKAEI
                     KDMNGDDDTPEPRRDERPREVEGRVVNDTRSTGSDPSRPAADPVRESERRD"
     gene            8541..9440
                     /gene="tatC"
                     /locus_tag="VLC50_00775"
     CDS             8541..9440
                     /gene="tatC"
                     /locus_tag="VLC50_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006501916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HSF26028.1"
                     /translation="MTETTQRRRLGRRRARRRDPEGRMSLGDHLRELRSRVVKAALAV
                     VVAGVVGWVWYEPIIDLLSHPLDVGRSARGGTGLVTLNFAGIADPFTLKLKIAFFVGL
                     LIASPVWIYQLWAFITPGLHRNERRYALGFLSAAVPFFLLGAWLAFAVMPKAIEFLIS
                     LTPTDGSNVVDASFYFTFVTRFILAFGIAFLLPVAMVGVNMAGLVSARRMARAWRISV
                     LAIFVFAAAATPTPDAGTMLLLGGAILFLYVLALGICFLTDRRKARRASAEPSASWSD
                     DEASPLEPVTHDPADDRPSRLDD"
     gene            9509..>10075
                     /locus_tag="VLC50_00780"
     CDS             9509..>10075
                     /locus_tag="VLC50_00780"
                     /inference="COORDINATES: protein motif:HMM:NF012981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HSF26029.1"
                     /translation="MSDEVALLVNPTAGKGRGARVGKLAATMLRQAGVPTRLLIGRDA
                     ADTAELGRAALEHGARALVVVGGDGMTHLGAQLVAGTTTPFGVVPAGTGNDFVRSLDL
                     PHRKPDVVISRLVEALRTGSTRPVDAVQAGATWYACVLSAGFDSMVNERANRLRWPTG
                     PRRYDIAMLLELPVFRPIPFVLELSDGDG"
BASE COUNT         1456 a         3654 c         3507 g         1458 t
ORIGIN      
        1 tcgacctggc cggtcgcgac tcgctgcagc gggtgccgac gctcgagccg ttgcgcggca
       61 cgcgtgcgca cgtcggggac ctgctggacc gctgcgacac cgccgaagac ctggtccgcg
      121 cgatcactgg cgcctgactc agctcggcga gtcgggcccc gtgtcggcgc cgacttgtag
      181 gctcaaagcc agcaggcact caaccccgcc acgccccttc gcgcggtggg accgaggagg
      241 cgagatggcg accaaggacg cgggcggcca gcagcgggtc acccgccgcg aagacaccga
      301 agagaccgcc ccccaggaga cggccgacct gaccgagcgc aaggaaaagc tcgacgacga
      361 catcgactcg atcctcgacg agatcgacga ggcgctcgaa gagaattcgg aggagtttgt
      421 gcgcggattt gttcagaaag ggggagagta gagacaaatc aagcgcggcg cgaccgcgaa
      481 agctactttc gacgcaagta cggcctgacc gaggccgaga tcgagcaacg acgtgaggac
      541 caggccggac gttgcccgat ctgccttgct ggccctgccg tccatgtcga ccacgaccac
      601 gagaccggtg ccgtgcgcgg actcgtctgc ttccagtgca atgccgcgct cggtcagctc
      661 cgcagcccct tcgtgatccg tcgagctgcc gcttacctcg agggcaccct cgcctcgccc
      721 agacgtgttg cgacgggagt attcgccctc ctgccccaca cggcgatccg cgaagctctt
      781 gcccccactg ctgacgcgtg accgggatcc gttcacacct gcagcgtcag agagcagcta
      841 agcaagtagg gtcgccgtcg tgtccttcaa ccccgatcgg gcgggcctgc ccgccgcctt
      901 tctcgctgcc gggtcgtctt cgttcaccga gttcctgggc cgctacgccc ccgacctact
      961 cccggcccga cgcaccctgc cggcggccgg catgaccatc gaggcaccgc acggtacgac
     1021 gatcgtcgcc gtgagcttcc ccggcggggt ggtcatggcc ggtgaccgtc gagccaccat
     1081 gggcaactac atcgcctcgc gcgacatcga gaaggtgttc cccgccgacg actactccgt
     1141 ggtcggcatc gccgggaccg ccgggatcgc ggtggaggtg gtccggctgc tgcaggtcga
     1201 gctcgagcac tacgagaaga tcgagggagc ggtgctgtcc ctcgacggca aggccaaccg
     1261 gctggcccag atgatccgcg gcaacctggc gatggcgatg cagggactgg ccgccgttcc
     1321 cgtgtttgcg gggtttgacc tcgagaccag cgaagggcgc atcttcagct acgacgtcac
     1381 cggcggccgc tatgaagagc accggttcca ctcggtgggg tcgggctcga tgttcgcccg
     1441 cggcgcgctc aagaagctct accgcgacga cctgaccgcc gaggacgccg cactggcctg
     1501 catcgagtcg ctctacgacg ccgccgacga cgactcggcg accggtgggc ccgacctgac
     1561 ccgccagatc ttcccgatcg tgtcggtggt cacgagcgag ggctaccgcc gcctgcccga
     1621 cgaggaggtc ggcgacctcg tgcgtctggt ggtcgaccgc cgcatgcagg atccaggagg
     1681 ccccaaggcg ccgctgcgct gaccccgacg ccaccgattc caccgacctg accggaccca
     1741 gcccacccac cggagccgca gtgaccacgc cgttctacgt ctcgcctgag cagctgatga
     1801 aggaccgggc cgactatgcc cgcaagggga tcgctcgagg ccgcagcgtg gtcgtcgtgg
     1861 cgtacgacgc cggcatcctg ttcgtcgccg agaacccttc gcgcgccctg cacaagatca
     1921 gcgagatcta cgaccggatc gcgctggccg cagtcgggcg ctacaacgag ttcgagaacc
     1981 tgcgcatcgc cggggtgcgc tacgccgacc tgcgcggcta ctcctacgac cgccgcgatg
     2041 tcaccgcccg gggcctggcc aatgcgttcg ctcagacgct cggcaccgtg ttctcaggcc
     2101 cgggggagaa gccgttcgag gtcgagctgg tcgtcgccga ggtgggggag agcaccgccg
     2161 ccgaccagct ctaccggctc acctacgacg gctcggtggg cgacgagcac ggctgggtcg
     2221 cgatgggcgg gcaggccgag accgtgggcg cggcgctcga ggagcactac cgcgacgggt
     2281 tggggctgca ggaggcgctc gccctggccg tgcaggcgct gggccgcgac gccaacgggg
     2341 cgcagcgcga gctcaccccc gaccagctcg aggtcgcagt gctcgaccgg caccggccgc
     2401 ggcgcaagtt ccgccggctg cagggctcac tgctcgagcg actgctcggc gaggccagcc
     2461 ccgcggtgga ggctgcggcg gcggctgaga tcgccgaggc ggacgacacc gacccgcctc
     2521 gccgctaacg cccgccgcgc cgcgaaccga cgtaccgtag ggcgatggac cggcgaatct
     2581 tcgggctcga gaacgagtac ggcgtcacgt gcacgtttcg cgggcagcga cggctgagcc
     2641 ccgacgaagt cgcccgctac ctgttccgcc gggtcgtctc ctggggtcgc agctccaacg
     2701 tgttcctgcg caacggagcc cggctctacc tcgacgtcgg ctcccacccc gagtacgcca
     2761 cccccgagtg cgacgacgtg cgccagctgg tgactcacga caaagctggc gagcggatcc
     2821 tcgagggcct gctcgtcgac gccgagcgcc ggctgcgcga ggaaggcatc gccggcgacg
     2881 tctacctgtt caagaacaac accgactcgg ccggcaactc ctacggctgc cacgagaact
     2941 acctggtcgg ccgccacggc gagttctccc ggctggccga cgtactcatc ccgttcctcg
     3001 tcacccgcca gatcatctgc ggcgcgggca aggtgctgca gaccccccgg ggggcggcct
     3061 actgcgtcag ccagcgggcc gagcacatct gggagggagt ctcctcggcg accacgcgat
     3121 cccggccgat catcaacacg cgagacgagc cgcacgcgga cgccgagcgc taccggcgcc
     3181 tgcatgtgat cgtcggcgac tccaacatga gcgagaccac gacgatgctc aaggtcggtg
     3241 ccaccgacct ggtgctgcgg atgatcgagt cgggcgtgat cctgcgcgac ctgacgctgg
     3301 agaacccgat ccgctcgatc cgcgaggtca gccacgacat caccggacgc cgagtcgtgc
     3361 gcctggccaa cggccgagag gccagcgcgc tggacatcca gggcgagtac ctcgccaagg
     3421 cccgcgactt cgccgcccgt cgcggcatcg acgagggcat cgtcaagcag gtgctcgacc
     3481 tgtgggagcg cacgctgcac gcgatcggca ccgacgacct cgacctggtc gggcgcgaga
     3541 tcgactgggt catcaagcac cggctgatcg agcgctaccg gatcaagcat ggtctctcgc
     3601 tcgcgtcccc ccggatcgcg gcgctcgacc tggcctacca cgacatccac cgcggccgtg
     3661 gggtctacta cctgctcgag cgggccggca aggtcgaacg cttcacgcgc gacctcgaca
     3721 tcttccaggc caagtcggtg ccgccccaga ccacccgggc ccggctgcgt ggagagttca
     3781 tccggcgggc tcaggagaag cgtcgtgact tcaccgtcga ctgggtgcac ctcaagctca
     3841 acgaccaggc ccagcgcacc gtgctgtgca aggacccgtt ccgctcggtc gacgagcggg
     3901 tcgaccggct gatccagagc atgtgaccgc tcggtctgcc cccgggcgtg agaccgaggc
     3961 caccagccgg aacgatcggg ccgagtgact cgtcgggtta gggtgtcagg tcgcctctcc
     4021 cagccgctca caccctctcg aggtccgctc gtgcgccgta ccaccatgtc cgtgctcgca
     4081 tcgctcaccg cgctcgctct gctgagcgcc tgcggctccg gcagctcgga cgccacgcca
     4141 agcggcagcg ccacgcccgc cgcgacgccg accttcgccg tgacctccga cgggccggcc
     4201 ccggcagtga acggcaaggc cggcagcgcg ccgaccctgg gcccgaccga gggcaagccg
     4261 tcggacaagc tcatcgtcaa ggagctcacc cccggcaagg gccgcgccat cgccaagggt
     4321 gacctcgtgc tcaccgactt cctcgtgcag aagtggggcg aggacaagcc gatcggctcg
     4381 acctacgccg aaggggcgcc gacctcgttc ccgatcggga tgagccaggt catcccagca
     4441 tgggaaaagc tcatcggcac caaggtcggc acccgggtcg agatggtcgt gccgccggcc
     4501 gctggcttcg gtgcggccgg caacgagcag gccgggatca aggcgaccga cacgctggtc
     4561 ttcgtcttcg acgtgcgtgg tgcctacgcc gccgatgcca aggccaacgg cgcccccggg
     4621 gagaggccgg cggcaggcct accgacggtc gccgacggcg aaaagggccc ggtcatcacc
     4681 gtgcccaagg gcgccaaggc ccccacgacc atctcgagca cccggctgat cacgggtgag
     4741 ggcgccgagg tgaagtcggg gcagaccatc gtggtgcagt acaccggtgc gctgtgggac
     4801 aagggcaccg tcttcgactc gtcgtgggag cgcggggcgc cggcctcctt cgtcatcggc
     4861 aagggcgcgg tcatcccggg ctgggacgag acgctggtcg gcacccacgt cggtgaccgg
     4921 gtcatgctcg tgatcccgcc cgacaaggcc taccgtgacc aggcgcaggg cgcgatcccg
     4981 gccaactcca cgctggtctt cgtcgtcgac gtcatcgacg ccctctgagc cagaccctct
     5041 cagcaccggc ccaccatcag cgaccaagga gtcccatgcc ccagctcgag aagcccgaga
     5101 tcgactttcc ggccggcgac gcgccgacca cgctgcaggt cgaggacctc gtcgtgggcg
     5161 acggcgccga ggccagcgcg ggtgccacgg tgctggtgca ctacgtcggg gtcgacttcg
     5221 cctccggcga ggagttcgac gcgtcgtgga accgtgggga gccgatcgag ttcccgttgc
     5281 gcgggctcat caagggctgg caggagggca tcccgggcat gaaggttggc ggccggcgca
     5341 agctcgtcgt accgcccgat ctggcctatg ggccggccgg ggcgggccac cggctcagcg
     5401 gtcagacgct catcttcgtg atcgacctgc tcgccgtgcg ctgaccctgg cgctgcccgg
     5461 gcgggccggt ctgagcctgc gtgacctggg tcgcccggcg cggtagcgtc cgcccccgga
     5521 ggagggggtg ctcgaccgtg tcatccaacg tgtcgcggaa caagacggag cggctgctca
     5581 acctgacgat cgccttgttg gcgacccgtc ggttcctgtc ccgcgaggag atccggacca
     5641 tggtgcccgg ctactccgag cgcgacgagg cgttcgagcg cgccttcgag cgcgacaagg
     5701 acgacctgcg cgagatgggg atcccgatcg agaccggctc caactcggcg ctgttcgacg
     5761 acgagcccgg ctatcgcatc cgtcgcgacg cctacgcgtt gcccgaggtc acgttcaccg
     5821 gcgacgagat ggccgtgctg gcggtggccg cgcaggcctg gtcgcacgcg gcgctcggcg
     5881 cgccggccgc gagcgcgttg cgcaagctca aggcctacgg cgtgggcctc gacgatgtcg
     5941 tggtcgcggg gctggccccg cgcgtggaga cccgcgaacc ggccttcgcc cggttgtggg
     6001 aggcggtgcg cgacgcgcgt ccggtgcggt tcggctaccg ccgctcgggc gcggacacca
     6061 ccgaggagcg caccgtcgag ccgtggggga tcgtctcgtg gcggggacgc tggtacgtcg
     6121 tgggccacga ccgtggccgg gaggcgaccc gagtgttccg cctctcgcgg gtgagggggg
     6181 aggtgaccct gctgggacgc cccggccagg tcgtgccccc gtccgacgtc gacctgcacg
     6241 cggcggtggt cgcggtcgcc gcggcccctg cccggggcac cgctcaggtg cggctgcgtc
     6301 gcggagccgg tggcttcctc cggcgccgag ccacggcagt caccgccgcc gatgccgagt
     6361 gggaggtgct cgacgtgccc tatggcgacc tccgtcagct cggcgaggag ctggcctcac
     6421 tcggcgccgc tgcggtggcg ctgggtccgg cggagctgcg ggccgaggtg tgcgggcggt
     6481 tgcgtgccgc ggccgggctc gaggcagcat catgatcccg gccaccgcac ggctggaccg
     6541 gctgctggcc ctcgtgccct atctgctcgc ccgtcccggt gtgcgggtcg aggacgtcgc
     6601 cgccgacttc ggcatcagcc ccgagcgact ggtcgacgac ctccagctgc tgttcgtgtg
     6661 tgggctgccc gggcacctgc ccgacgacct gatcgacgcg tccttcgagg gcggcgtcgt
     6721 gcacgtgacc aacgccgaca cgatcgcccg cccgctccga ctggccgccg acgaggcggt
     6781 ggcgctgctt gccgggctgc ggatgctggc cgcactgccc ggcgcggtcg gccacgacgc
     6841 gctcgaccgg gccctggtca agctcgagcg cgccgcgggt gatgcggcgg cggccaccga
     6901 gcggatgacg ctctcggtcg aggccgagga cgaggtggtg gcgacggcac gggccgggct
     6961 cgaccagggt cgggcgttga ccctcgacta ctacgtatcc ggtcgtgacg agacgacgcg
     7021 ccggaccgtc gacccgatgc ggctgctcgt cgtcgatggc cgggcctacc tcgaggcctg
     7081 gtgccgccgg gccgaggcgg tgcggctgtt ccgggccgac cgggtcgagc acatcgccgt
     7141 cctcgacgag ccggccgctg tcccagcctc tgcgcagcag cgggacctct cgctgggtct
     7201 gttccagccg accgccgacg acgtggtgat cacgctcgac ctcacgcgga acgcccggtg
     7261 ggtggccgag cactacccct gcgaggacgt cgaggacttg ccgggcggtg ggctgcgggt
     7321 gcggctgcgt acccccgagc cgcgctgggt ggtccgtctg gtgctgcggc tgggtgccga
     7381 cgggcagatc gtcgacccgc cgactctcgc cgccgaggtg cgcgggcagg ccctggcggc
     7441 gctggccgcc tacgaagaga cctcggtcaa ctcggctcaa gactgaggcg ccggtggccg
     7501 aatcctcagg tgagcccgca cccagggcga ggcacgagga ggccgacagt gacgaccatc
     7561 aaggcgagct gcccgtcgtg cggcgacgtc gacctcaccc cccgccagct gcgcctggtc
     7621 gtcgcgacga agccggggat gtcctactac tcgttcagct gcactcattg ccgcgacatc
     7681 gtgcagcggc ccgcgggcga tgacgtcgta cgcatcctgg tctcgggggg agtgcggccc
     7741 gagccgtggg acgtgccggc cgaggtgctc gagcagcacg acggcccggc cctgacctac
     7801 gacgacctgc tcgacctggc cctggcgctg caccgcatgg accgcctggt ccttgctctg
     7861 gccccgagca ccggagcccg cgcctgaggc gccaccggag gcgcgaggcc accggctagg
     7921 gtcagggccg tggtgtggtt cctcgtctgg gtcgctctgg tggccggtgc actcctggtc
     7981 atcgggctgc tggtccgccg gctctggcgg gcggtcaagg cgctcggcgc cgagctggca
     8041 accaccactg cccagctcga ggagatcacc gccaggctcg agtcacttcc cgacctgcca
     8101 cgctgagcaa ccaccggcgc tgtgcctggc cgaccggccg acgtacgatg acgccaacga
     8161 cgtcgatgtg aggggcgccc gacatgcgtg gactctttga gggctggcac ctggtcatcc
     8221 ttctggtgct gatcctgctg ctgtttgggg ccaagcgcct gcctgacgcc gctcgcgggc
     8281 tggggcgttc gatgcgcatc ttcaaggccg agatcaagga catgaacggc gacgacgaca
     8341 cgccggagcc gcgccgtgac gagcgtcccc gcgaggtcga gggccgcgtc gtcaacgaca
     8401 cccggtccac cggcagtgac ccgagccggc cagccgccga ccccgtgcgg gagtccgagc
     8461 gccgcgactg atcgacgcgc cgtgccactc gctcggcccg tcccttcgcc ctcggcccgc
     8521 cctccctaca tcgacctgtc atgaccgaga ccacgcagcg ccgccgcctc ggacggcggc
     8581 gggcacgtcg tcgcgacccc gaggggcgga tgtcgctcgg cgaccatctg cgcgagctgc
     8641 gctcccgggt ggtcaaggcg gcgctggccg tcgtggtggc cggggtcgtg ggctgggtct
     8701 ggtacgaacc gatcattgac ctgctctcgc acccgctcga cgttggccgg tccgcgcgcg
     8761 ggggcaccgg cctggtcacg ctcaacttcg cgggcatcgc cgacccgttc acgctcaagc
     8821 tcaagatcgc gttcttcgtc ggtctgctca tcgccagccc ggtctggatc taccagctct
     8881 gggccttcat cacaccgggc ctgcaccgca acgagcgccg ctatgccctg ggcttcctgt
     8941 cggcggccgt cccgttcttc ctgctcggcg cctggctcgc gttcgccgtc atgccgaagg
     9001 cgatcgagtt cctgatctcg ctgacaccca ccgatggcag caacgtcgtc gacgccagct
     9061 tctacttcac gttcgtgacc cggttcatcc tggcgttcgg catcgccttc ctgctgccgg
     9121 tggccatggt cggggtcaac atggcggggc tggtcagcgc ccgccggatg gcccgggcct
     9181 ggcggatcag cgtgctggcg atcttcgtgt tcgccgcggc ggccaccccg acccccgacg
     9241 ccggcaccat gctgctgctc ggcggggcga tcctgttcct ctatgtcctg gcgctgggca
     9301 tctgcttcct caccgaccgc cgcaaagccc ggcgggcgag cgccgagccc tcggcgtcct
     9361 ggagcgacga cgaggcctcg ccgctggagc cggtgaccca cgacccggcc gacgaccggc
     9421 ccagccggct cgacgactga cccgcacaac cccctcaccg gtacggcgtg cgccggagcg
     9481 gccgcaccga ttggtagcgt cgccgcccgt gagcgatgag gtggccctgc tggtcaaccc
     9541 gactgcgggc aagggccgcg gcgcccgggt cggcaaactg gccgccacga tgctgcgcca
     9601 ggccggcgtg cccacccgac tgctgatcgg ccgcgacgcc gccgacaccg ccgagctcgg
     9661 ccgagccgcc ctggagcacg gggcgcgtgc cctggtcgtc gtcggcggcg acggcatgac
     9721 ccacttgggt gcccagctgg tcgccggcac gaccacgccg ttcggggtgg tgccggccgg
     9781 caccggcaac gacttcgtgc gctcgctcga cctgccgcac cgcaagcccg acgtggtgat
     9841 ctcccgcctg gtcgaggcgc tacgcactgg atcgacccgc ccggtcgacg cggtgcaggc
     9901 cggtgccacc tggtatgcgt gcgtgctctc ggccggcttc gactcgatgg tcaacgaacg
     9961 tgccaaccgg ctgcgctggc ccactggacc gcgccgctac gacatcgcca tgctgctcga
    10021 gctgccggtg ttccggccga tcccgttcgt cctcgagctc tccgacggcg acggc
//
LOCUS       DATIBB010000022        10265 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_1587820, whole genome shotgun sequence.
ACCESSION   DATIBB010000022 DATIBB010000000
VERSION     DATIBB010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 10265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10265)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10265
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1587820"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1680
                     /locus_tag="VLC50_00785"
     CDS             <1..1680
                     /locus_tag="VLC50_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020423159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HSF26030.1"
                     /translation="RDFGVRPTDMHRPMVDELVRPNPDDPTGRSMMRRVPEVLDCWFE
                     SGSMPFAQVHYPFENADWFEHHYPGDFIVEYIGQTRGWFYTLHVLATALFDKPAFDTC
                     VVHGILLGSDGRKMSKSLRNYPDVTEVFDRDGSDAMRWFLMSSPVLRGGELVVTEAGI
                     REGVRQVIKPLWNAWHFFALYANAAGGPGNNGGYDARWRTDSPHVLDRYVLAKTHDLV
                     LTLTEQLDQYDVSGACTSVREHLETLTNWYIRRSRQRFWDAEPAAFDTLYSVLQVLTR
                     LAAPLLPLTTESVFRGLTGERSVHLTDWPAPDELPGDDGLVAAMDAVRDVCSATLGLR
                     KAAHLRVRLPLAKLTVATEHAADLAPFVDLVRGEVNVKDVVLTTEAESGLAVQRVLHV
                     NPRVLGPRLGKDVQTVIKACKSGDWSVAPDGAVTVGGFALTEGEYDVELSLAGATDGS
                     AVTATLPTGGFVVLDTEVTPELAAEGIARDVVRAVQQARRDAGLHISDRIRLGVDGDA
                     EALTAVDEHRDMVAGETLATEVVIGAVPAERAAEVQVGDGLAVRLTVEKSA"
     gene            1866..3158
                     /locus_tag="VLC50_00790"
     CDS             1866..3158
                     /locus_tag="VLC50_00790"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018385592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HSF26031.1"
                     /translation="MLARAPEHHVQPSLERIQALVDLLGNPQRAFPVVHITGTNGKTT
                     TARIIEALLREFGLRTGRFTSPHLHEMTERIAIDGEPVGAAAFAQAWQELRPLVELVD
                     ARSDTAMSYFEVLVGLAYAIFADAPIDVAVVEVGLGGAWDATNVADGQVAVITPIAID
                     HTNYLGSTVEAIATEKAGIIKAGSVAVFAEQPTGAAAVLTARGLAVGARVVREGVDFG
                     VLSRTLAVGGQQLSLQGLGGTYDELFLPLHGAHQAHNAAAAVAAVEAFLGGGHDRLDP
                     SVVRAAFAGVTSPGRLEVVRRSPTVLVDAAHNPAGALATAAAIGEEFGFTRLVGLLAI
                     FADKDVEGILEAFEPVLTEVVVTEMVSPRAMPIDELADIAEGIFGVGRVTAVPRLDAA
                     LDEAIRRAEAEGAGAGAGVLVSGSITTVAGARALLGAY"
     gene            3179..3544
                     /locus_tag="VLC50_00795"
     CDS             3179..3544
                     /locus_tag="VLC50_00795"
                     /inference="COORDINATES: protein motif:HMM:NF025383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4233 domain-containing protein"
                     /protein_id="HSF26032.1"
                     /translation="MQPRPTRRILASATLTMEALLVVFAVIVAANFSSVPTTTVIVVG
                     AAAALACVVVAGLLRYSWAYGAGWVLQALIVLSGLVVPMMWFLGAVFVLLWWLALHYG
                     AKAERIQAAFRDHEGLPPS"
     gene            complement(3572..4270)
                     /locus_tag="VLC50_00800"
     CDS             complement(3572..4270)
                     /locus_tag="VLC50_00800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF26033.1"
                     /translation="MQLTFMIMWGALIGSRRTAIVLALGLALAGCTPEDDSAGPTPTP
                     VPTSAPSPATTPNRQAPASASTTTATPGKAISCLDGEWSFGAVQRQRVVSHVTEPLVI
                     TSSDPVQPPPLQPLRRVVAEVEGNAPPELAYARVRELLPPELGLPPLDARLEDGESGA
                     SNLMTEPGVVVFYELADVISVDYRYTCEGRPDIGTVTTWAAMGGGILRCGGSHPFDPP
                     DIADVRAIACPRVR"
     gene            complement(4296..4793)
                     /locus_tag="VLC50_00805"
     CDS             complement(4296..4793)
                     /locus_tag="VLC50_00805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF26034.1"
                     /translation="MATPGDAISCLDGEWSFGAAQRQRIVSHVSEPVQVTSGVPVQEP
                     PLRPLRRVVATVEGNAPPELTYAQVRRLLPEVSLPALGARAEDSEPGFSYGSDQPELL
                     VFYELADVISVDYRYTCQGRPDYGSVTTWTGMGRGVVRCDGVQPSDPPEVADVHAIAC
                     PADAS"
     gene            5156..5563
                     /gene="ndk"
                     /locus_tag="VLC50_00810"
     CDS             5156..5563
                     /gene="ndk"
                     /locus_tag="VLC50_00810"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HSF26035.1"
                     /translation="MSERTLVLIKPDAVRRGLVGQVLSRLERKGLSLVAMELRQIDGE
                     ASDRHYAEHVERDFYPPLRAFITSGPLVALVAEGDQAIAVVRALTGATDGRTAAPGTI
                     RGDFALSNRENLMHASDSPESAAREIALWFPGL"
     gene            5684..6715
                     /locus_tag="VLC50_00815"
     CDS             5684..6715
                     /locus_tag="VLC50_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009188595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HSF26036.1"
                     /translation="MANNMSFIGRDMAVDLGTANTLVYVRGKGIVLNEPSVVAINTNT
                     GGILAVGVEAKKMIGRTPGNIVAIRPLKDGVIADFDTTERMLRYFIQKVHRRRKMAKP
                     RIVVCVPSGITGVEQRAVKDAGYAAGARKVYIIEEPMAAAIGSGLPVHEPTGNMVVDI
                     GGGTTEVAVISLGGIVTAQSIRIGGDELDSSIIQYVKKEYSLMLGERTAEEIKCAIGS
                     AFPMPDEPHAEIRGRDLVSGLPKTIVVSAEEIRKAIEEPVNQIVDAVKTTLDKCPPEL
                     SGDIMDRGIVLTGGGALLRGLDERLRHETGMPIHITDKPLDSVAMGSGKCVEEFDALQ
                     QVLISEPRR"
     gene            6768..7700
                     /gene="mreC"
                     /locus_tag="VLC50_00820"
     CDS             6768..7700
                     /gene="mreC"
                     /locus_tag="VLC50_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018088259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005618 - cell wall [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HSF26037.1"
                     /translation="MRRDSRRTRIILVLLLLTAFTLITLDYQRSGTSPLTGVRSAAAA
                     VFGPVERAAAAVVRPVSEFVTNLGDLGEHQDRIEELQAEIDRLRQDNRTSELARNRAG
                     ELDGLLRVAGAGQYRVLPARVVAVGAKQEFSWTVTIDAGTRDGISRDLTVLNGAGLVG
                     RVVEVGPWTSTVLLAVDPDFRAGVRLESSMDIGYVTGKGERPLTLDLLGGQARVAKGD
                     RLVTQGSVNDRPFVPGVPVGEVTSVQATPGSPTKTATVRPFVDFARLDLVGVVVQPPR
                     DNPRDGVLPPRPAAATPTPVPTVTVTVTAPPPEG"
     gene            7702..8217
                     /gene="mreD"
                     /locus_tag="VLC50_00825"
     CDS             7702..8217
                     /gene="mreD"
                     /locus_tag="VLC50_00825"
                     /inference="COORDINATES: protein motif:HMM:TIGR03426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HSF26038.1"
                     /translation="MRTSRALLSAVLLLVALLVQVVFASRVPLPGAATPDVVLVVVVA
                     LGLVRGPLHGCVAGFAGGLALDLVPPADHAAGRWALVLCLVGYIAGLFDHEVDRSAFF
                     PFVAVATASASATLLYAGLGLVIGDTSATWDMIVGVLPSAVLYDVILTPFVMLAVAGL
                     ARRVATDPLRR"
     gene            8231..>10265
                     /gene="mrdA"
                     /locus_tag="VLC50_00830"
     CDS             8231..>10265
                     /gene="mrdA"
                     /locus_tag="VLC50_00830"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HSF26039.1"
                     /translation="MSERSRLRLVVLQVLIVSLLATLGGRLWFLQVVNAETYIKAADN
                     TSLREVITPATRGLILDDQGRPLATNRTALVVSVSRAALAEQKDGGTDLIARVAAVIG
                     KPFDEVWGHTRLCGEAGAPKPPDCWNGSPFQPIPVTDVASTEMALQIMEHAEDFPGVT
                     AEITTIRDYPLPEKANAAHILGYLGPVTDAELEAQKKNPKAETRLQRTDLVGRLGLEA
                     HYDEELRGTPGVTTVTIDRRNLVTGTVSEVAPTPGNYLVTSIDARVQAVTEKALIDSI
                     KRARTTGDPNKRGAKYKADSGAVVVLDVNTGRIVAMASYPSYDPNVWVGGISKKEYDA
                     IASKKNNYPNQSRAFQGQFAPASTFKLISTPAAVEAGYSLKGSYPCPSAYPIGNSMKR
                     NYESKGYGTLTFRRAIQVSCDTMFYKFAYETWLRQGGTKAPIGTKDPFIEMAKAFGLG
                     KRTGLDLPGEAPGRIGDRQWKYDFWESRRDYNCTMAADGYKEMAKTDPENAKFLTQLA
                     KENCAPSGYVYRAGDAANTAIGQGDTTVTPLQLARAYAAVANGGTLYVPTVGKAIVKP
                     DGTLVREIKPQVAGKLPISKATLAWMQDTFTAVTQPGGTGYRPFVNAGFPLDQIPVAS
                     KTGTGEVHGKQSTSWFASWAPANKPQYAVVMMISQGGTGSGVSGPGVAEIYTAL"
BASE COUNT         1480 a         3635 c         3675 g         1475 t
ORIGIN      
        1 cgcgacttcg gcgtacgtcc gaccgacatg caccggccga tggtcgacga gctggtccgg
       61 cccaaccccg acgacccgac cgggcgctcg atgatgcgcc gggtgccgga ggtgctcgac
      121 tgctggttcg agtcgggctc gatgccgttt gcccaggtgc actacccgtt cgagaacgcc
      181 gactggttcg agcaccacta cccgggcgac ttcatcgtcg agtacatcgg ccagacccgc
      241 ggctggttct acacgctgca cgtgctggcc accgcgctgt tcgacaagcc ggcgttcgac
      301 acctgcgtgg tgcacgggat cctgctcggc tccgacggcc gcaagatgag caagagcttg
      361 cgcaactacc ctgacgtcac cgaggtcttc gaccgcgacg gctccgacgc gatgcgctgg
      421 ttcctgatgt cctcgccggt gctgcgcggc ggcgagctcg tcgtcaccga ggccggcatc
      481 cgtgagggcg tgcgccaggt catcaagccg ctgtggaacg cctggcactt cttcgcgctc
      541 tacgccaacg ccgccggggg accgggcaac aacggcgggt acgacgcccg ctggcgcacc
      601 gacagcccgc acgtgctcga ccgctacgtg ctggccaaga cccacgacct ggtgctcacg
      661 ctgaccgagc agctcgacca gtacgacgtg tccggggcct gcaccagcgt gcgcgagcac
      721 ctggagacgc tgaccaactg gtacatccgc cggtcccgcc agcggttctg ggacgccgag
      781 ccggcggcgt tcgacacgct ctactcggtg ctgcaggtgc tgacccggct ggccgcgccg
      841 ctgctgccgc tgaccaccga gtcggtcttc cgcggcctga ccggcgagcg cagcgtgcac
      901 ctgaccgact ggccggcccc cgacgagctg cccggcgacg acggcctggt cgcggcgatg
      961 gacgccgtac gcgacgtctg ctcggccacc ctcgggctgc gcaaggccgc ccacctgcgg
     1021 gtccggctgc cgctggccaa gctcaccgtg gccaccgagc acgccgccga cctggcgccg
     1081 ttcgtcgacc tggtccgcgg cgaggtcaac gtcaaggacg tcgtgctcac cactgaggcc
     1141 gagtcgggcc tggcggtgca gcgggtgctg cacgtcaacc cccgggtgct cggcccccgg
     1201 ctgggcaagg acgtccagac ggtgatcaag gcctgcaagt ccggcgactg gagcgtcgcc
     1261 ccggacggtg cggtgaccgt gggcgggttc gcgctgacgg agggggagta cgacgtcgag
     1321 ctgtcgctgg ccggcgcaac cgacggcagc gcggtgaccg cgaccctgcc gaccggcggg
     1381 ttcgtcgtgc tcgacaccga ggtcaccccc gagctggccg ccgagggcat cgcccgcgac
     1441 gtggtccgcg cggtgcagca ggcccgccgc gacgccgggc tgcacatctc cgaccggatc
     1501 cggctcggcg tcgacggcga cgccgaggcc ctgaccgccg tcgacgagca ccgggacatg
     1561 gtcgccggcg agacgctggc caccgaggtg gtcatcggcg cggtgccggc cgagcgggcc
     1621 gccgaggtcc aggtcggcga cgggctcgcc gtacgcctca ccgtggagaa gtccgcgtga
     1681 gcgaggccaa tggcgccggt cccgagcggg gcgatccgag catcctggcc cacctgctcg
     1741 gcgacgacgt ggcgccgggg gaccgcgacg agcgcgacga gctcgacgag gccgggcgct
     1801 ccgacgaggg cgacgccgac cgggccgagg ccgagctggc ggcgttcgcc caggcccggg
     1861 ccgagatcct cgcccgcgcc cccgagcacc acgtgcagcc ctccctcgag cggatccagg
     1921 ccctcgtcga cctgctgggc aacccgcagc gggccttccc ggtcgtgcac atcaccggca
     1981 ccaacggcaa gaccaccacg gcgcgcatca tcgaggcgct gctgcgcgag ttcgggctgc
     2041 gcaccggccg gttcaccagc ccgcacctgc acgagatgac cgaacggatc gccatcgacg
     2101 gcgagccggt cggcgcggcg gctttcgccc aggcctggca ggagctgcgc ccgctcgtgg
     2161 agctggtcga cgcgcgcagt gacaccgcga tgagctattt cgaggtgctg gtcgggctgg
     2221 cctatgcgat cttcgccgac gcgccgatcg acgtcgcggt cgtcgaggtg ggcctgggcg
     2281 gggcctggga cgcgaccaac gtcgccgacg gccaggtcgc ggtgatcacc ccgatcgcga
     2341 tcgaccacac caactacctc ggctcgaccg tcgaggcgat cgcgaccgag aaggccggca
     2401 tcatcaaggc cggctcggtg gcggtgttcg ccgagcagcc gactggggcc gccgcggtgc
     2461 taaccgcccg cgggctggcg gtcggtgccc gcgtcgtacg cgagggcgtg gacttcggcg
     2521 tgctctcccg gaccctggcc gtcggcgggc agcagctgag cctgcagggg ctcggcggca
     2581 cctacgacga gctgttcctg ccgctgcacg gcgcgcacca ggcgcacaac gccgccgccg
     2641 ccgtcgccgc ggtcgaggcg ttcctcggcg ggggtcacga ccggctcgac ccctcggtgg
     2701 tgcgggccgc gttcgccggc gtcacctctc cgggtcggct cgaggtcgta cgacggtccc
     2761 cgacggtgct cgtcgacgcg gcgcacaacc cggccggcgc gctggccacc gcggcggcga
     2821 tcggcgagga gttcggcttc acccggctgg tcgggctgct cgcgatcttc gccgacaagg
     2881 acgtcgaggg catcctggag gcgttcgagc cggtgctgac cgaggtggtc gtcaccgaga
     2941 tggtctcgcc gcgggcgatg ccgatcgacg agctggccga catcgcagag ggcatcttcg
     3001 gggtcgggcg cgtcactgcg gtgccgcggc tggacgcggc gctggacgag gcgatccggc
     3061 gggccgaagc cgagggggcc ggcgccggag ccggcgtgct cgtctccggc tccatcacca
     3121 cggtggccgg ggcccgggcc ctgctcggcg cctactgacc gtggcggaag gagcacccgt
     3181 gcagccccgg cccacccggc ggatcctggc ctcggcgacg ctgacgatgg aggcgctgct
     3241 cgtcgtcttc gcggtgatcg tcgcggccaa cttctcgtcg gtgccgacga cgacggtgat
     3301 cgtggtgggc gcggcggcgg cgctcgcgtg cgttgtggtg gccgggttgc tgcgctactc
     3361 ctgggcctac ggcgccggct gggtgctgca ggcgttgatc gtgctcagcg gcctcgtcgt
     3421 acccatgatg tggttcctcg gcgcggtgtt cgtgctgctg tggtggctgg ccctgcacta
     3481 cggcgccaag gccgagcgca tccaggccgc cttccgtgat catgaaggtt taccgccctc
     3541 ctagggcgag ttttccttca tgatcgcggg cctacctcac gcgcgggcag gcgatcgccc
     3601 gcacgtcagc gatgtccggc gggtcgaacg gatgggatcc gccgcaccgc aggatgccgc
     3661 cgcccatcgc ggcccaggtg gtgaccgtcc cgatgtcggg tcggccctcg cacgtgtagc
     3721 ggtagtcgac cgagatcacg tcggccagct cgtagaagac gacgacgcca ggctcagtca
     3781 tcaggttgga ggcgccggac tctccgtcct cgaggcgcgc gtcgagggga ggcaggccca
     3841 gctcgggcgg gaggagctcg cgcacccggg cataggcgag ctcgggtggc gcgttgccct
     3901 cgacctcggc cacgacacgg cgtagaggtt gcagcggggg cggctggacc ggatcgctgc
     3961 tagtgatcac cagcggttcc gtgacgtgac tgacgaccct ctggcgctgg acggcgccga
     4021 aggaccactc gccgtcgagg caggagatcg ctttgcccgg agtggccgtg gtggtcgagg
     4081 cgctcgcggg cgcctggcgg ttgggcgtgg tcgccggaga cggagcactc gtggggacgg
     4141 gcgtgggtgt gggtccggct gagtcgtctt cgggcgtgca cccggcgagc gccaggccga
     4201 gcgcgagcac gatcgccgta cggcgtgatc cgatgagcgc cccccacatg atcatgaagg
     4261 ttagctgcac cccaaggcca agtaatgctt catgatcacg atgcgtcggc ggggcaggcg
     4321 atcgcgtgca cgtcggcgac ttcgggcggg tcggacggct ggacgccgtc gcacctgacg
     4381 actccccgac ccatcccggt ccaggtggtg accgacccgt agtcgggccg accctggcag
     4441 gtgtagcggt agtcgaccga gatcacgtcg gccagctcat agaagacgag caactcgggc
     4501 tggtcgcttc cataggagaa gccgggttcg ctgtcctcgg cgcgggctcc caaggcaggc
     4561 aggctgacct ccgggagcag gcggcgcacc tgcgcgtagg tcagctcggg cggcgcgttc
     4621 ccctcgaccg tggcgacgac tcggcgcagg ggtcgcagcg ggggttcttg gaccgggacg
     4681 ccactcgtga cctgcacggg ttcgctgacg tgactgacga tcctttggcg ctgggcggcg
     4741 ccgaaggacc actcgccgtc gaggcaggag atcgcgtcac cgggagtggc catggtggtc
     4801 gaggcgctcg cgggcgccgg gcggttgggc gcggtcgcgc cagacgaagc gctcgtgggg
     4861 acgggcggcg gagcggatcc ggctgaatcg ccttcggccg tgcacccggc aagcgctagg
     4921 ccgagcgcga gcacgatcgc cgtacggcgt gatccgctgc gcgccccccg catgatccgg
     4981 aagactagcc tccctcctgg ggcgagtcag ccttcatgct catggggagg gtgcgcggcg
     5041 ggctccgatt gcttgatcac cgcgtgaggg tgggcacgga ttgcttgatc accaagaggg
     5101 gtcggtaggc tcgcgggcgc tttcagaacc gtccacgacc cccgggagcc cgttcgtgtc
     5161 cgagcgcacg ctcgtcctga tcaagcccga tgcggtgcgc cgcggcctgg tggggcaggt
     5221 gctgtcccgg cttgagcgca aggggctctc gctggtggcg atggagctgc gccagatcga
     5281 cggcgaggcg tccgaccggc actacgccga gcacgtcgag cgcgacttct acccgccgct
     5341 gcgcgcgttc atcacgtcgg gtccgctggt cgcactggtg gccgagggcg accaggccat
     5401 cgcggtggtc cgggcgctga ccggggccac cgacgggcgc accgctgcac ccggcaccat
     5461 ccgcggcgac ttcgcgctgt ccaaccggga aaacctcatg cacgcctcgg actcacccga
     5521 gtcggcggct cgcgagatcg ccctctggtt ccccggcctc tgaccgccag acacgccgcg
     5581 ggcgcggtcg gggtcttcgg ctacggctac catggagccc ggtcggcggg gtgagagcgc
     5641 ggccgacgtg atagccgact tctaccggtg aggcctgttt ctcatggcaa acaacatgtc
     5701 gttcatcggt cgtgacatgg cggtcgacct cggcaccgcc aacaccttgg tctatgtgcg
     5761 cggcaagggc atcgtgctca acgagccgag cgtcgtcgcg atcaacacca acaccggcgg
     5821 catcctcgcc gtcggggtcg aggccaagaa gatgatcggc cgtacgccgg gcaacatcgt
     5881 ggccatccgc ccgctcaagg acggtgtgat cgccgacttc gacacgaccg agcggatgct
     5941 gcgctacttc atccagaagg tccaccgccg ccgcaagatg gccaagccgc gcatcgtcgt
     6001 ctgcgtgccc agcggcatca ccggcgtcga gcagcgcgcg gtcaaggacg ccggctacgc
     6061 cgccggggcc cgcaaggtct acatcatcga ggagccgatg gccgcggcga tcggctcggg
     6121 cctgccggtg cacgagccga ccggcaacat ggtcgtcgac atcggcggcg gcaccaccga
     6181 ggtggcggtg atctcgctcg gcggcatcgt caccgcacag agcatccgca tcggcggcga
     6241 cgagctcgac agctcgatca tccagtacgt caagaaggaa tactccttga tgctcggcga
     6301 gcgcaccgcc gaagagatca agtgcgcgat cggctcggcg ttcccgatgc ccgacgagcc
     6361 gcacgccgag atccgcgggc gcgacctcgt ctctgggctg cccaagacga tcgtggtcag
     6421 cgccgaggag atccgcaagg cgatcgagga gccggtcaac cagatcgtcg acgcggtcaa
     6481 gaccaccctc gacaagtgcc cgcccgagct gtccggcgac atcatggacc gcggcatcgt
     6541 cctcaccggc ggcggtgcgc tgctgcgcgg gctcgacgag cggctgcgtc acgagaccgg
     6601 catgccgatc cacatcaccg acaagcccct cgactcggtg gcgatgggct cgggcaagtg
     6661 cgtcgaggag ttcgacgcgc tgcagcaggt gctcatctcc gagccccggc gctgaccgtg
     6721 acggcctcgg gggggccgcg gaccgccgcg gttcgtgggc cggattcgtg agacgtgact
     6781 cccgacgcac gcggatcatc ctcgtgctgc tgctgctcac cgccttcacg ctgatcaccc
     6841 tcgactacca gcgcagcggc acgtcgccgc tgaccggggt ccgctcggcc gcggcggcgg
     6901 tgttcgggcc ggtcgagcgc gcggcggcgg ccgtcgtacg cccggtgtcc gagttcgtca
     6961 ccaacctcgg cgacctgggc gagcaccagg accgcatcga ggagctgcag gccgagatcg
     7021 accggctgcg ccaggacaac cgcaccagcg agctggcccg caaccgggcc ggcgagctcg
     7081 acgggctgct gcgggtggcc ggcgccgggc agtaccgcgt gctgccggcc cgggtggtgg
     7141 ccgtcggggc caagcaggag ttctcctgga cggtcaccat cgacgccggc acccgcgacg
     7201 gcatcagccg cgacctgacc gtgctcaacg gcgccgggct ggtcgggcgg gtcgtcgagg
     7261 tcggcccgtg gacctcgacc gtgctgctcg ccgtcgaccc cgacttccgg gccggggtgc
     7321 ggctggagag ctcgatggac atcggctacg tcaccggcaa gggtgagcgc ccgctcacgc
     7381 tcgacctgct cggcgggcag gcgcgcgtcg ccaaggggga ccggctggtg acgcagggct
     7441 cggtcaacga tcgaccgttc gtccccgggg tgccggtcgg cgaggtgacc agcgtgcagg
     7501 ccacgcccgg ctcgccgacc aagaccgcga cggtccgccc gtttgtcgac ttcgcccgcc
     7561 tcgaccttgt cggcgtggtc gtgcagccgc cgcgggacaa cccacgcgac ggcgtgctgc
     7621 cgccgcgacc ggcagccgcg acgcccacgc cggtgccgac ggtgacggtc acggtgaccg
     7681 cgccgcctcc ggagggctga gatgcgtacg tcgcgggcgc tgctctcggc ggtgctgctg
     7741 ctggtcgcgc tgctggtgca ggtggtgttc gcctcgcgag tgccgctgcc cggggccgcc
     7801 acccccgacg tcgtactcgt cgtggtggtg gcgctcggac tggtgcgcgg gccgctgcac
     7861 gggtgcgtgg ccggcttcgc cggcggcctg gcgctcgacc tggtgccgcc ggccgaccac
     7921 gccgccggcc gctgggcgct ggtgctgtgc ctcgtgggtt acatcgccgg gctgttcgac
     7981 cacgaggtcg accgctcggc gttcttcccg ttcgtcgcgg tggccaccgc ctcggcctcg
     8041 gcgacgctgc tctatgccgg tctcggtctg gtgatcggcg acaccagcgc gacctgggac
     8101 atgattgtcg gggtgctgcc ctccgctgtt ctctacgatg tgatcctgac cccgttcgtc
     8161 atgctggccg tggcggggct ggccaggcgg gtcgcgaccg acccgctgcg tcgctagtcc
     8221 ggagccgtca gtgagcgagc gttcccgcct tcgcctggtg gtcctgcagg tcctcatcgt
     8281 gtcgttgctg gccaccctcg gcggccggct gtggttcctg caggtggtca acgccgagac
     8341 ctacatcaag gccgccgaca acacgtcgct gcgcgaggtg atcaccccgg ccacccgcgg
     8401 gctgatcctc gacgaccagg gccgcccgct ggccaccaac cggaccgcac tggtcgtctc
     8461 ggtgagccgc gccgcgctgg ccgagcagaa ggacggcggc accgacctga tcgcgcgggt
     8521 cgccgcggtg atcggcaagc cgttcgacga ggtgtggggc cacacccgcc tgtgcggcga
     8581 ggcgggcgcg cccaagccgc ccgactgctg gaacggctcg ccgttccagc cgatcccggt
     8641 caccgatgtc gcgtcgaccg agatggccct gcagatcatg gagcacgccg aggacttccc
     8701 cggggtgacc gccgagatca ccacgatccg cgactacccg ttgcccgaga aggccaacgc
     8761 cgcgcacatc ctcggctatc tcggcccggt caccgacgcc gagctggagg cgcagaagaa
     8821 gaatcccaag gccgagacgc ggctgcagcg caccgacctg gtcggccggc tgggtctgga
     8881 ggcgcactat gacgaggagc tgcgcggcac cccgggcgtg accacggtga ccatcgaccg
     8941 gcgcaacctg gtcaccggca ccgtctccga ggtggcgccg accccgggca actacctcgt
     9001 gaccagcatc gacgccaggg tgcaggcggt caccgagaag gcgctgatcg actcgatcaa
     9061 gcgggctcgc accaccggtg accccaacaa gcgcggggcg aagtacaagg ccgactccgg
     9121 cgccgtggtg gtcctcgacg tcaacaccgg ccggatcgtc gcgatggcgt cctacccctc
     9181 ctatgacccc aacgtctggg tgggtgggat ctccaagaag gagtacgacg cgatcgcgag
     9241 caagaagaac aactacccca accagtcgcg cgccttccag gggcagttcg cgccggcgtc
     9301 cacgttcaag ctgatctcca cccctgcggc ggtggaggcg ggctattcgc tcaagggctc
     9361 ctatccgtgc ccgtcggcct atcccatcgg caactcgatg aagcgcaact acgagtcgaa
     9421 gggctacggc accctgacct tccgccgggc catccaggtc tcctgcgaca cgatgttcta
     9481 caaattcgcc tacgagacct ggctgcgcca gggcggcacc aaggcgccga tcggcaccaa
     9541 ggacccgttc atcgagatgg ccaaggcgtt cggcctcggc aagcgcaccg gcctcgacct
     9601 gcccggtgag gcgccagggc gcatcggcga ccggcagtgg aagtacgact tctgggagtc
     9661 ccgccgcgac tacaactgca cgatggccgc tgacggctac aaggagatgg ccaagaccga
     9721 cccggagaac gccaagttcc ttacccagct ggccaaggag aactgcgcgc cgtccggcta
     9781 cgtctaccgg gccggtgacg cggccaacac cgcgatcggg cagggcgaca ccaccgtcac
     9841 cccgctgcag ctggcccggg cctacgccgc ggtggccaac ggcggcacgc tctacgtgcc
     9901 caccgtcggc aaggcgatcg tcaagcccga cggcaccctg gtccgcgaga tcaagccgca
     9961 ggtggccggc aagctgccca tctccaaggc caccctggcg tggatgcagg acaccttcac
    10021 cgcggtgacc cagcccggcg gcaccggcta ccgcccgttc gtcaacgcgg gtttcccgct
    10081 cgaccagatc ccggtggcca gcaagaccgg caccggtgag gtccacggca agcagtcgac
    10141 gtcgtggttc gcctcctggg cgccggcgaa caagccgcag tacgccgtgg tcatgatgat
    10201 cagccagggc ggcaccggga gcggcgtctc cggcccgggc gtggccgaga tctacacggc
    10261 gctct
//
LOCUS       DATIBB010000023         8335 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_404382, whole genome shotgun sequence.
ACCESSION   DATIBB010000023 DATIBB010000000
VERSION     DATIBB010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 8335)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8335)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8335
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_404382"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1105
                     /locus_tag="VLC50_00835"
     CDS             <1..1105
                     /locus_tag="VLC50_00835"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HSF26040.1"
                     /translation="GAAPQIPPLSAEDVERRRVRSGLAALLPRLTGHLAPVIEAGQMV
                     VVTDTEGRVLWRRGNPRIKREADRLGFVGGSAWTEGNVGTNAIGTALVLGAPVHVRGP
                     EHFADSHTRWGCAAAPLVDPWTGCTLGVVDVSGPSRSLHPTELAVVAMAARLATLELR
                     DERAARLERLRAVAAPVLAQVRGKVVVVDPDGHVAAVSGMSPPAVISLPKHLRVGPVW
                     LPTLGEATAEVLPGGWLLRLGRAPLQAGAATEIVLDLSGAVPELRVTGPSGRWQRALS
                     LRHAEIVVALLRAEQGRSPAELAADLFADATRVVTVRAEMSRLRRVMGALLESRPYRL
                     ADGVTGRLLLPDAARVLPGSSAPAVTRLRAQRR"
     gene            1155..1472
                     /locus_tag="VLC50_00840"
     CDS             1155..1472
                     /locus_tag="VLC50_00840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF26041.1"
                     /translation="MTVRLRAAVVRRPSLPLTERDEQELAALREHRTHLHVLEQLSGV
                     PVTGGTSEAALLHAVFQAGLAAVREAAEEAGYAEIAAASADRAPEARAAARRRRPTWA
                     DEA"
     gene            1469..1804
                     /locus_tag="VLC50_00845"
     CDS             1469..1804
                     /locus_tag="VLC50_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006594374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HSF26042.1"
                     /translation="MSEIWPLVRGRVYAARMSHLEEDKYFLVVSNNRRNRQLPQVLAV
                     RLTTTPKPPLPSIVPLVRPEVFVGSVVCDDIVEIYPDEVRRDLGALSPAAMAAVGRGL
                     AAALGLERP"
     gene            complement(1817..2233)
                     /locus_tag="VLC50_00850"
     CDS             complement(1817..2233)
                     /locus_tag="VLC50_00850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF26043.1"
                     /translation="MDEPTTYRGVLGEVTRQVALPHAYTLLLWAVTTVTIIRDGFPTI
                     TGVLLLLVGAGTGYFGISLLCGPSRGTARVALHRKTPRLSRVPTLMLFAAVGLCWLLT
                     LALSSVLVWFAVGLVATVVYLVGLSLQIFTVTHRAS"
     gene            2490..2810
                     /locus_tag="VLC50_00855"
     CDS             2490..2810
                     /locus_tag="VLC50_00855"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HSF26044.1"
                     /translation="MPHEPRRRGPRDAGPWAGFGAGVARRRRELGLTQRDLADLADVA
                     VATVQGLEAGRLATRLGSLAAILDALGLTLTAVPSDRAAQIGADDANAIRLTPPGSTP
                     GEVG"
     gene            2807..4051
                     /locus_tag="VLC50_00860"
     CDS             2807..4051
                     /locus_tag="VLC50_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018599558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA domain-containing protein"
                     /protein_id="HSF26045.1"
                     /translation="MSEQLDQLRWVEAADVYKGDRLAGQLVRDGDDVVFRYRADYLDD
                     PAAPALAWSLPKSAEPVRASAGSVPPFFAGLLPEGVRLRAVATGARTSEDDHLTLLLA
                     VGADAIGDVRVVPADRAPEDPGVLVDESKVDTLDLEDVFGQAVSTDPAALQRVALPGV
                     QVKVSAAMLSTPVTTTVGPAILKLNPPTDYPRLVHNEHFFLGVAQACRIPIPPCRLVH
                     DGKGHDALLVGRFDRVVRRDGVQRLPQEDACQVLGVYPARKYRLRTEDVARALADTVA
                     AGGGSRPLALRRVMELVVFSYLIGNGDLHGKNLSIRQVPQGRWEITPGYDLLSTQPYT
                     GWGNPMALDLFGRANRLDRAHLLDAARRLGLPERAMARTLDRLTDGVESCLPRLGDIG
                     LGDRATELLDRLVRRRLAEVRG"
     gene            4116..4784
                     /locus_tag="VLC50_00865"
     CDS             4116..4784
                     /locus_tag="VLC50_00865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF26046.1"
                     /translation="MSVRRGAGIATLPRREAATAVTRHPQLADPDAVEPVVVMVSARD
                     EAEAAELADLISLAKAALRSRNEAAHGVLRALLPEDQLSISAGVLTQLRHNAEAQRAL
                     AAEFGLLSSAEVGTIAGSKARNAAALASRWRTEGRIFGVPVDGSVRYPGFQLEDGKPR
                     PVVAETIKILGGHLAGWELALWFTGDNDWLGGVRPVDALTGTAADRDAVLAAAQHLAD
                     ELAD"
     gene            4807..5529
                     /locus_tag="VLC50_00870"
     CDS             4807..5529
                     /locus_tag="VLC50_00870"
                     /inference="COORDINATES: protein motif:HMM:NF020387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RES family NAD+ phosphorylase"
                     /protein_id="HSF26047.1"
                     /translation="MSAAPARRPVVRPPIPIPSAPRTAAALAGLDPLIARWPTGAAFF
                     HCYDLAWGPRDFFAGDPAHLGRFHPFRPAGAQAPVPVLYGASDDLGALSETVFHDVPV
                     RGTKHVPRAKLTRRALVELAPTRDLTLIDLTSDGLSRLGLARLELIESDARSYADTAQ
                     WARALHAHPAHVDGLTWVSRQHDTSRALVLFGDRVPRDDLAEREGVAPVALAAGAGLD
                     RVCEAADRAGITIAGLGEQPRR"
     gene            complement(5643..5807)
                     /locus_tag="VLC50_00875"
                     /pseudo
     CDS             complement(5643..5807)
                     /locus_tag="VLC50_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003910999.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
     gene            5873..6442
                     /locus_tag="VLC50_00880"
     CDS             5873..6442
                     /locus_tag="VLC50_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004558843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HSF26048.1"
                     /translation="MQGKLLALTYLHPDREYSLTEAAALVGASVKAVHQEASRLVEAG
                     LLADRRVGTARLVRVAGSPIQRQLTELIAVTYGPLPVLTDALRSVAGVHRAFIYGSWA
                     ARYHGEPGPVPADVDVLVVGTARRDDLDEVARSVEPILGRMVNIRRVKPAAWDAGDAT
                     DTFLATVRNRPLVELQVDGPEANGRAAAR"
     gene            6485..6589
                     /locus_tag="VLC50_00885"
                     /pseudo
     CDS             6485..6589
                     /locus_tag="VLC50_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006403071.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stability protein"
     gene            complement(6730..7131)
                     /locus_tag="VLC50_00890"
     CDS             complement(6730..7131)
                     /locus_tag="VLC50_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015493291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004867 - serine-type endopeptidase
                     inhibitor activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SSI family serine proteinase inhibitor"
                     /protein_id="HSF26049.1"
                     /translation="MRIVVLLALALVSACSGGGTSTASPGTGPSSQLTVTVTDGDTVR
                     VQTLTCDPTGGDHPDAAAACAVLDRLGAAAFAPVPADQACTEIYGGDQTATVTGVWRG
                     EPVDASYDRSNGCQIARWDAIVDVLQVRGGV"
     gene            complement(7195..>8335)
                     /locus_tag="VLC50_00895"
     CDS             complement(7195..>8335)
                     /locus_tag="VLC50_00895"
                     /inference="COORDINATES: protein motif:HMM:NF016047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HSF26050.1"
                     /translation="FAAGTRHTIADYATRCPLVRGIGLTVMRGATASVTSVRVDQNTP
                     EGVRSPQLSIFRQDPGAGWVYRVGGPDRIDTALRAARMLQARIGQFDAAVLGRADGFA
                     DNLAGTALAKTYRAPLLLTGRTQLDARVAKELTTSVRPGGTVYLLGGTAALSPQVEQA
                     VAALGFTVKRLAGADRFATAVAIFDQLVPHPEPEGQLCQVSLADGLNFPDALVAANLD
                     KPIIFSAGSTLPPTTSEFLRRCDVGRMVTVVGGPAARALAGSSYAGALWFRYVGADRY
                     GTSEAVLRAMAPTDRRPLLDLSMATGENFPDALVAATLGVTVLSPPDAPRESLHAIVS
                     DMAPYLNAIYLLGDTDVLSDTVGERVKSWMAGQTTGHIVSPAVEN"
BASE COUNT         1144 a         2961 c         2994 g         1236 t
ORIGIN      
        1 gggcgctgcg ccgcagatcc cgccgctgtc ggccgaagac gtcgaacggc gccgcgtgcg
       61 cagcgggctg gccgccctcc tcccgcggct gaccgggcac ctggcgccgg tgatcgaggc
      121 cgggcagatg gtggtggtca ccgacaccga gggtcgggtg ctgtggcggc ggggcaatcc
      181 gcggatcaag cgggaggccg atcggctggg cttcgtcggc ggctcggcct ggaccgaggg
      241 caacgtcggc accaatgcga tcggcaccgc gctggtgctt ggcgcgccgg tgcatgtgcg
      301 cggccccgag cacttcgccg actcgcacac ccggtggggc tgtgctgccg cgccgctggt
      361 cgacccctgg accgggtgca ccctcggggt cgtcgacgtc agcggcccgt cccgcagcct
      421 gcatccgacc gagctggccg tggtcgcgat ggccgcccgg ctggcgacac tcgagctgcg
      481 tgacgagcgc gccgcccggc tcgagcggct gcgggccgtg gccgcgccgg tgctcgccca
      541 agtgcgcggc aaggtggtcg ttgtcgaccc cgacggtcac gtcgcggccg tctctggcat
      601 gtctcccccg gccgtgatca gcctgcccaa gcacctgcgg gtcgggccgg tctggctgcc
      661 cacgctcggc gaggcgaccg cggaggtgct gcccggcggc tggctgctcc ggctcggacg
      721 cgcccctctc caggccggcg ccgcgaccga gatcgtcctg gacctgtccg gtgcggtgcc
      781 ggagctgcgg gtcaccgggc cgagcgggcg ctggcagcgg gccctcagcc tccgccacgc
      841 cgagatcgtg gtcgcactcc tgcgcgccga gcaaggccgc agcccggccg agctggccgc
      901 cgacctgttc gccgacgcca cccgcgtggt gaccgtgcgg gccgagatgt cccggctgcg
      961 ccgcgtcatg ggcgccctgc tcgagagtcg gccctaccgg ctcgccgacg gcgtcaccgg
     1021 ccggctgctg ctgcccgacg cggcccgggt gcttccgggt tcgagcgccc cggcggtcac
     1081 tcggctgcgt gcccaacggc gttgagggct actggtcttg gacagtgact acttagtgcg
     1141 gcacagtgtc aggcatgacc gtgagactgc gcgcggccgt ggtgcggcgg ccgtcacttc
     1201 ccctgaccga gcgcgacgag caagagctgg cggctctccg cgagcaccgc acccacctgc
     1261 acgttctcga gcagctgtcg ggcgtcccgg tgacgggtgg aacgagcgag gccgcactgt
     1321 tgcatgcggt gttccaagca gggctcgcgg ctgtccgtga agctgctgag gaagccggtt
     1381 acgccgagat cgcggccgcg tcggccgatc gggcgcccga ggctcgtgcc gccgctcggc
     1441 gccgacgccc gacgtgggcc gacgaggcgt gagcgagatc tggccgctgg ttcgaggccg
     1501 ggtctacgcc gcacggatga gtcacctcga ggaggacaag tacttcctcg tcgtgtcgaa
     1561 caaccggcgc aatcgccaac tgccgcaagt gctggctgta cggctcacca cgacccccaa
     1621 gccgccgttg ccgagcatcg tgccgcttgt gcggccggag gtcttcgtgg gcagcgtggt
     1681 ctgcgacgac atcgttgaga tctatccaga cgaggtacgg cgcgacctcg gtgctctcag
     1741 cccggcggcg atggctgcgg tcggccgcgg gctagctgcc gcactgggcc tcgaaaggcc
     1801 ctgagtcagc cggggcctaa cttgcccggt gggtcacggt gaagatctgc agcgagagcc
     1861 cgaccaggta gacgaccgtc gcgaccaggc cgaccgcgaa ccacacgagc acggacgaaa
     1921 gcgccagcgt gagcagccag cacagcccca ccgcggcgaa cagcatgagc gtcggcaccc
     1981 gcgacagccg cggcgtcttg cggtgcagcg ccacccgagc cgtcccgcgc gaaggcccgc
     2041 acaagaggct gatcccgaag tagccggtgc cggcgccaac gagcagcagc agcaccccgg
     2101 tgatcgtggg gaacccgtcg cggatgatcg tgacggtggt gaccgcccac agcagcaggg
     2161 tgtaggcgtg cggcagggcg acctggcggg tcacctcgcc gagtacgccg cgataggtgg
     2221 tcggctcgtc cacgtgcccg gtgctcctcc aacccacaac gcccgcgtac gtcgttcagg
     2281 tcggcgtgcg cggcggcatg gtagtcgccg gagacggagc gacctccagc gccccagact
     2341 cgccaaagca ggtttagggc cctcagggag aaacaagacg tgcggcggcg ccgcaaaacc
     2401 tgcttcagca aggttaggat tggtacaccc tcatcccatg ccaacggcgt gaaacaaaat
     2461 atggaatcac cagtttaagg agcggaagct tgccccacga accgcgtcgt cgggggcccc
     2521 gtgacgccgg tccctgggcc gggttcgggg ccggcgtcgc gcgtcggcgg cgggagctgg
     2581 ggctcaccca acgggatctg gcggacttgg ccgacgtcgc ggtggcgacc gttcagggac
     2641 tcgaggccgg tcggctggcc acgaggctcg ggtcgctcgc cgccatcctc gacgccctcg
     2701 gcctgacgct gaccgctgtc ccgtcggaca gggccgcgca gatcggcgcg gatgacgcca
     2761 atgcgatccg tctcacgccg cccgggtcga cgccggggga ggtcgggtga gcgagcagct
     2821 cgaccagctc cgctgggtcg aggctgcgga cgtctacaag ggcgaccggc tcgccgggca
     2881 actggtgcgt gacggtgatg acgtcgtctt ccgttaccgg gccgactacc tcgacgaccc
     2941 cgccgcgccg gccctggcgt ggagcctgcc caagtcggcc gaaccggtgc gggcttcggc
     3001 cgggtcggtg ccacccttct tcgcgggcct gctgcccgaa ggggttcggc tgcgggccgt
     3061 ggctaccggg gctcggacca gcgaggacga tcacctcacc ctgctgctcg cggtcggcgc
     3121 cgacgcgatc ggcgacgtac gcgtggtccc agctgaccgc gctccggaag acccaggcgt
     3181 gctggtcgac gaatccaagg tcgacaccct cgatctcgag gacgttttcg gccaagcggt
     3241 gtcgaccgat cccgcggcac tccagcgagt ggccctgccg ggcgtgcagg tcaaggtgtc
     3301 ggccgcgatg ctctcgacgc ccgtgaccac caccgtcggg ccggcgatcc tcaagctcaa
     3361 tccgcccacc gactatcccc ggctggtgca caacgagcac ttcttcctcg gcgttgccca
     3421 ggcctgccgc atcccgatcc cgccgtgccg gctggttcac gacggcaagg gtcacgacgc
     3481 gctgctggtc ggccgcttcg atcgcgtcgt acggcgcgat ggagtgcagc gactgccgca
     3541 ggaggatgcg tgccaggtgc tcggggttta cccggcgcgg aaataccggc tgcgcaccga
     3601 ggacgttgcg cgggcgctcg ccgacacggt cgcggccggc ggcgggtcgc ggccgctggc
     3661 gctgcgtcga gtcatggagc tggtggtgtt tagctacctg atcggcaacg gcgacctgca
     3721 tggcaagaac ctgtcgatcc ggcaggtccc gcaaggccgg tgggagatca cgcccggcta
     3781 tgacctgctg tccacccagc cctacaccgg ctggggtaac ccgatggctc tcgacctgtt
     3841 cggccgggcc aaccggctcg atcgcgcgca cctgctcgac gccgcgcgac ggctcggtct
     3901 gccggagcgg gcgatggcgc gcacactcga ccgcctcacc gacggcgtcg agtcgtgcct
     3961 gccgcgtctc ggcgacatcg ggctcggcga ccgggcgacc gagctgctcg atcggcttgt
     4021 gcgccgtcgt ctcgccgagg tccgtggcta gccgcgccgc cagcatgagg ttcatgacgc
     4081 ttgacgccaa tgctcatgtg aatcattatc cttgcatgag cgtacgacgg ggcgcaggca
     4141 tcgcgaccct tccgcgccgg gaggcagcga ctgcggtcac gcggcatcct cagctggccg
     4201 accccgatgc cgtcgagccg gtcgtcgtca tggtctccgc ccgtgacgag gccgaagccg
     4261 ccgagctggc cgacctgatc agcctggcga aggcggcgct gcgctcgcgc aacgaggcgg
     4321 cgcacggcgt gctgcgcgcg ctgctgcccg aagatcagct gtccatcagc gctggagtcc
     4381 tgacccagct gcgccacaac gccgaggccc agcgcgcgct ggccgcagag tttgggctgc
     4441 tgtccagcgc cgaggtcggc accatcgccg ggtccaaggc tcgcaatgcc gcggccctgg
     4501 cgtcccggtg gcgcaccgag ggccggatct tcggcgtgcc cgtcgacggg tcagtgcgct
     4561 accccggctt ccagcttgag gacggcaagc cgcgcccggt cgtcgcggag acgatcaaga
     4621 tccttggcgg ccacctcgcg ggctgggagc tcgcgctgtg gttcaccggc gacaacgact
     4681 ggctcggcgg ggtccggccg gtcgacgcct tgaccggcac ggctgctgac cgcgacgcgg
     4741 tgctggcggc tgcgcagcac ctcgccgacg agctggccga ctgacggtct ggcacggagt
     4801 cgacgggtga gcgcagcacc ggctcggcgg cccgtcgtac gtcctccgat tcccatccct
     4861 tcggccccac gcaccgcagc ggcactcgcc ggcctggacc cgttgatcgc ccggtggccg
     4921 accggggcgg ccttcttcca ctgctacgac ctcgcctggg ggccgcgcga cttcttcgcc
     4981 ggagacccgg cccacctcgg tcgcttccat cccttccggc cggcaggggc gcaggcgcca
     5041 gtgccggtcc tgtacggcgc cagcgacgat ctcggtgccc tcagcgagac cgtctttcat
     5101 gacgtgccgg tccgcggcac caagcacgtg ccccgggcca agctgacgcg tcgtgcgctg
     5161 gtggagctgg ccccgacccg agacctgacg ctgatcgatc tgaccagcga cgggctgtcg
     5221 cggctcggcc tggcccggct cgagctcatc gagtcggacg cccgcagcta cgccgacacg
     5281 gcccagtggg cgcgggcgct gcacgcccat cccgcgcacg tcgacgggtt gacctgggtc
     5341 agccgccagc acgacacgag tcgggcgctg gtgctttttg gtgaccgggt cccccgcgac
     5401 gacctcgccg agcgcgaggg cgtcgcgccc gtggcgctgg cggccggggc cggcctcgac
     5461 cgggtgtgtg aggccgccga ccgtgccggc atcaccatcg ccggtctggg agagcaaccc
     5521 cgtcggtgag ccggggctcg ctcacacgcg gatgagctcc attccgggta cgtcggctac
     5581 cgcggtgaag tcggcgtcct gcgtgacgag cggcgggcga ggtgcccgtg tggtcactgc
     5641 agttcggatt tggagatcca agtacggtgc tcgcgctccg cgtcgacgct gaccagcgca
     5701 gcgaccgggc gcccggcgac ggtgatgacg atctcctcgc cggcctctgc ccgtcgcagc
     5761 agggcggctg tgttgttgcg cagctcgctg gaggcgacgt gggccatgac ctcgagtatt
     5821 acctctcatg cgaactgcag caccagtcct gctgccgctg ctgcggtccc aggtgcaggg
     5881 caagctcttg gcgctgacct atctgcatcc cgaccgtgaa tacagcctca ccgaggcggc
     5941 ggcgctggtg ggggcatcgg tcaaggctgt tcatcaagag gcatcccgtt tggtcgaggc
     6001 cggcctgctc gcagatcgcc gtgtcgggac tgcgcgtctg gtccgggtcg ccgggtcccc
     6061 gattcagcga caactcacgg agctgatcgc cgtcacctac ggtccgctgc cggtgctgac
     6121 ggacgccctc cggagtgtgg ccggcgtgca ccgtgccttc atctatggat cttgggcggc
     6181 gcgctaccac ggcgagcccg gtccggtgcc ggccgacgtg gacgtgctgg tcgtgggcac
     6241 ggcacgtcgc gacgaccttg acgaggtggc acgaagcgtg gagccaatcc tcggccgcat
     6301 ggtcaacatc cgtcgggtga agcctgctgc atgggacgcc ggagacgcca cggatacgtt
     6361 cctcgcgaca gttcgcaacc gaccgctggt ggaactgcag gtggatggtc ccgaagccaa
     6421 tggtcgggcg gctgctcgat gagatgtcgc cgttctgatg ccgcggtggc ccgtcccgag
     6481 cgccgtccgt ctcggctcgc tcctcatcga gatcggtcgc gatgtcggtg gtgtcgacct
     6541 cgacatcact cgcgacaaga cccccgccga gccgccgagc tttgagtgat cgtcgtcgac
     6601 ccccacgtca tctccgagct gctgcggcca cgcccggctg gctctgtggt gtcatggctc
     6661 gatggtcgcc acccgcgaca cgacgccgtt cgccgcggcg gggctgcgag tcatcaaccc
     6721 gttcgccggc tagaccccgc cgcgcacctg cagcacgtcg acgatcgcgt cccagcgcgc
     6781 gatctgacac ccgttgctcc ggtcgtaaga cgcatcgacc ggctcgcccc gccacacccc
     6841 agtgactgtc gcggtctggt cgccgccgta gatctcggtg cacgcctggt cggccggcac
     6901 cggcgcgaac gccgccgcac cgagccggtc gagcacggcg cacgcggcgg cggcgtcggg
     6961 gtggtcgccg ccggtcgggt cgcaggtcaa ggtctgcacg cgcaccgtgt cgccgtcggt
     7021 gaccgtgacc gtcagctggc tgctaggccc ggtgccaggt gacgcggtcg acgtcccccc
     7081 gcccgagcag gcactgacca gagccagggc cagcagtacg acgatccgca tgccgcttcg
     7141 acgccgcacg gttggccccg gttccgcccc ggcgaccagc ggccgcggtc acgatcagtt
     7201 ttcgaccgcg ggagagacga tgtgcccggt ggtctggccg gccatccacg acttcacccg
     7261 ctcccccacg gtgtcgctga gcacgtcggt gtcgccgagc aggtagatcg cgttgaggta
     7321 gggcgccatg tccgacacga tcgcgtgcag gctctcgcgc ggcgcgtccg gcggcgacag
     7381 cacggtcacg ccgagcgtgg cggcgaccag cgcgtccggg aagttctccc cggtggccat
     7441 gctcaggtcg agcagcggcc gccggtcggt cggcgccatg gcccgcagca ccgcctccga
     7501 ggtgccgtag cgatccgcgc cgacgtagcg aaaccacaga gcaccggcgt acgacgaccc
     7561 cgccagggcg cgcgccgccg gcccgccgac gacggtgacc atgcggccga catcgcatcg
     7621 gcgcaggaat tcggaagtgg tcggcggcag ggtggatccg gcgctgaaga tgatcggctt
     7681 gtcaaggttg gcggcgacca gggcgtcggg gaagttgagc ccgtcggcca gggagacctg
     7741 gcagagctgc ccttcgggtt ccgggtgggg caccagctgg tcgaagatcg cgaccgcggt
     7801 ggcgaaccgg tcggctcctg ccagccgctt caccgtgaag ccgagcgcgg cgaccgcctg
     7861 ctcgacttgc gggctcagcg ccgcggtgcc gccgagcaga tagacggtcc cgcccggccg
     7921 gaccgaggtc gtcagctctt tagccactcg agcgtcgagc tgggtccgcc cggtcagcag
     7981 gagcggcgcg cggtaggtct tggctagggc ggtgccggcc aagttgtcgg cgaacccgtc
     8041 ggcgcgtccg agcaccgccg cgtcgaactg gccgatccgg gcttggagca tccgggccgc
     8101 gcgcagagcc gtgtcgatcc ggtctggtcc gccgacccgg tagacccatc cggcaccggg
     8161 gtcctggcgg aagatgctga gctgcggcga ccgcactccc tcgggagtgt tctggtcgac
     8221 cctgacgctc gtcaccgacg cggtggcgcc gcgcatcacg gtcagcccga tgccacgaac
     8281 gagggggcag cgggtcgcgt agtcggcgat ggtgtgccgg gtgccggcgg cgaac
//
LOCUS       DATIBB010000024         5355 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_141887, whole genome shotgun sequence.
ACCESSION   DATIBB010000024 DATIBB010000000
VERSION     DATIBB010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 5355)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5355)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5355
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141887"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(132..204)
                     /locus_tag="VLC50_00900"
     tRNA            complement(132..204)
                     /locus_tag="VLC50_00900"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(169..171),aa:Asn,seq:gtt)
     gene            complement(288..803)
                     /locus_tag="VLC50_00905"
     CDS             complement(288..803)
                     /locus_tag="VLC50_00905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF26051.1"
                     /translation="MRFRGRVPDAVHPWVPLAPGERVLSWGRTPEGWYAVATTHALHR
                     RGPDGDPGAGLATPYAEILRARWQPETSTLDLVEAVEGSTRRVVLTLEEPGLLPETVR
                     ERVQRSILASRRIQVRGSKGVTVVARRPAAGRGPVAWQVSFDTGIDESDPAVADQVDA
                     ALAELRATLGV"
     gene            862..1731
                     /locus_tag="VLC50_00910"
     CDS             862..1731
                     /locus_tag="VLC50_00910"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012948141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HSF26052.1"
                     /translation="MTIAITGATGHLGRLVVAALLDRGVPPEQIVAVARDTAKAADLV
                     ERGITVRYGDYDRPDSLVGALAGADRVLLVSGSEVGRRVPQHQAVIEAARQHDVELLA
                     YTSIPQADTTPMRLAAEHRATEQLLRESGLPYALLRNSWYVENYTGQLASFLEHGAIL
                     GAAGTGRISGAPRADYAGAAAAVLTGEGHEKAVYELGGDEAFTLAELAAVISACTGRE
                     IGYQDLPVAAFEQALVSAGLPAPVAATFADVDACIAAGALHVTTGDLSRLLGRPTTSL
                     RDAVAQAVASYQG"
     gene            complement(1750..3000)
                     /gene="rpoD"
                     /locus_tag="VLC50_00915"
     CDS             complement(1750..3000)
                     /gene="rpoD"
                     /locus_tag="VLC50_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015882413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HSF26053.1"
                     /translation="MASPDRPVVHSPPAPRRHRSAGAAPPVLVVQRRVVTAEGGMVPQ
                     VVRTEDGRDGEQAPDLVEEPAPEELAQVEAEHGAESVSVEAGAGEAIEGTESARAVDD
                     AEDDEPPERVTLDGPSADLVRVYLREIGRVALLTAEQEVQLARRIEAGVFAAELLASG
                     ERAEIAGELQAVRADGERAKSHLVEANLRLVVSIAKRYAGRGLPLLDLVQEGNLGLIR
                     AVEKFDYTKGYKFSTYASWWIRQAISRAVADQARTIRVPVHMVETVNRLLRTQRQLAQ
                     DLGREPSDEDLSNHLDLPVERIQELRRLAMEPVSIHTPVGEDEGSELGDLIEDAESPE
                     PIDVVSAGMLAGHIEAVLACLGERERQVVRLRYGLTDGNPHTLEEVGQAFGVTRERVR
                     QIEAKSLAKLRHPQLSSQLRDFLG"
     gene            complement(3155..5056)
                     /gene="dnaG"
                     /locus_tag="VLC50_00920"
     CDS             complement(3155..5056)
                     /gene="dnaG"
                     /locus_tag="VLC50_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013131442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HSF26054.1"
                     /translation="MAGRIRDEDVALVKERVNIADVIAEHVTLRNAGGGNLKGLCPFH
                     DEKTPSFSVRPAVGSFHCFGCGEGGDAISFVMKLEHLSFAETVERLAAKAGITLRYDE
                     GGGPTGLHPGRRARLVAAHRAAAEFYAEALASPEAAPGRAFLAERGFDREVAARFGVG
                     WAPTGWDALARHLRGRGYDADELVTAGLVRQGQRGPIDYFRGRLLWPIRDIRGDVVGF
                     GARRLLEEDRIAAKYVNTPETPIYKKSQVLYGLDLARKPIATTHQAVIVEGYTDVMAC
                     HLAGVQTAVATCGTAFGQEHIKVLRRLLMDTTDALGEMAGEVVFTFDGDAAGQKAALR
                     AFADDQRFVAQTFVAVEPSGMDPCELRQHRGDDAVRALVAARVPLFEFVIRTTLRKHD
                     LEIPEGRVAALRSAAPIVAAIRDRSLRPEYARRLAGWLGMEVAPVLDAVSRAARTRPG
                     APAGHPSAHQPDPAPEPPTPAIARPDPRDPATAVERESLKAVLQLPALAAGFDALPAS
                     CFTSPAYAAVRAVVAACGGVAAAGAQAGWVDRVRTAAADPPVAALVTELAVEPLRADT
                     APDGRYVAALLARLEELDLTRGAADLHSRLQRLDATEDADAYAAVFSELVALETRRRD
                     AHERAAGSG"
     gene            5172..>5355
                     /locus_tag="VLC50_00925"
     CDS             5172..>5355
                     /locus_tag="VLC50_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006502297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHC2 zinc finger domain-containing protein"
                     /protein_id="HSF26055.1"
                     /translation="MAGRIKDEDVALVKERVNIADVVAEHVTLRNAGGGNLKGLCPFH
                     DEKTPSFSVRPSVGSFH"
BASE COUNT          716 a         1962 c         1964 g          713 t
ORIGIN      
        1 cgccggcggg tgtaggtctt gcgcagtccg aaagtctcaa tcaccgcagg cacgtcgtcc
       61 cccttcgaca cggggacggt aacagcgctg ccatcgacgg atgcgcacca gctgcgccgg
      121 cgtggcggat gctcccgcga taggactcga acctataacc ctccggttaa cagccggatg
      181 ctctgccaat tgagctacgc gggaccgggt cgccgtcggc gaccacgacc gcacaggtta
      241 gcgcaccggc cgcaggccgc cgaaccgcgc cgggcgcccc ccggggctca cacgccgagc
      301 gtggcgcgca gctcggccag cgccgcgtcg acctggtcgg cgacggctgg gtcggactcg
      361 tcgatgccgg tgtcgaaact gacctgccag gcgaccggtc cgcgcccggc agccggtcgg
      421 cgggccacga cggtgacccc cttcgacccg cgcacctgga tccgccggct ggccaggatg
      481 ctgcgctgga cccgctctcg gaccgtctcg ggcagcaggc cgggctcctc gagcgtgagc
      541 acgacgcgcc gggtggaccc ctcgacggcc tcgaccaggt cgagggtgga ggtctccggt
      601 tgccagcggg cccgcagaat ctcggcgtac ggcgtggcca ggccggcgcc gggatcgccg
      661 tcggggccgc gccggtgcag ggcgtgggtg gtcgcgacgg cgtaccagcc ctcgggagtg
      721 cgaccccagg acagcacgcg ctcgccaggg gcgaggggca cccacgggtg cacagcgtca
      781 gggacccggc cgcgaaatct cacaaaccca ccgtaggcga tgctgtgcag gccaccacca
      841 accgaggagc ggagcacacg catgaccatc gccatcaccg gagccaccgg gcacctgggc
      901 cgcctcgtcg tcgcggcgct gctcgaccgc ggcgtgccgc ccgagcagat cgtcgcggtg
      961 gcccgcgaca cggccaaggc agccgacctg gtcgagcgcg gcatcacggt gcggtacggc
     1021 gactacgacc ggccggacag cctggtgggt gcgctcgccg gcgccgaccg ggtgctgctc
     1081 gtgtcgggca gcgaggtcgg gcggcgtgtc ccccagcacc aggcggtgat cgaggcggcc
     1141 cgccagcacg acgtcgagct gctcgcctac acgtcgatcc cgcaggccga cacgaccccg
     1201 atgcggctgg ccgccgagca ccgcgccacc gagcagctgc tgcgcgagtc cgggctgccc
     1261 tatgccttgc tgcgcaacag ctggtacgtc gagaactaca ccggccagct cgccagcttt
     1321 ctcgagcacg gtgcgatctt gggcgccgcc gggaccggcc ggatcagcgg ggcgccgcgc
     1381 gccgactacg cgggcgccgc ggccgccgta ctcaccggag agggtcatga gaaggcggtc
     1441 tacgagctcg gcggcgacga ggcgttcacc ctggccgagc tggctgcggt gatctcggca
     1501 tgtaccggac gggagatcgg ctaccaggac ctgccggtcg cggcgttcga gcaggcgctg
     1561 gtgagcgccg ggctgccggc tccggtcgcg gcgacgttcg ccgacgtcga cgcctgcatc
     1621 gccgcgggcg cactgcacgt aacgaccggt gacctgtccc ggctgctcgg gcggccgacg
     1681 accagcctcc gggacgccgt cgcgcaggcg gtcgcgtcgt accagggctg atcacccggg
     1741 accgagcggt cagccgagga agtcgcgcag ctggctggac agctgcgggt ggcgcagctt
     1801 ggccagcgac ttggcctcga tctggcggac ccgttcgcgg gtcaccccga aggcctgccc
     1861 gacctcctca agggtgtgcg ggttgccgtc ggtgagcccg tagcgcagcc ggaccacctg
     1921 ccgctcccgc tcccccaggc aggccagcac cgcctcgatg tgcccggcca gcatgcccgc
     1981 ggagaccacg tcgatcggtt cgggtgactc ggcgtcctcg atgaggtcac cgagctcgga
     2041 gccctcgtcc tcgccgaccg gggtgtggat cgagacgggc tccatggcca gccggcgcag
     2101 ctcttggatc cgctcgaccg gcaggtcgag atggttggac aggtcttcgt cgctgggctc
     2161 gcggcccagg tcctgggcca gctggcgctg ggtgcgcagc agccggttga cggtctcgac
     2221 catgtggacc ggcacccgga tggtgcgggc ctggtcggcc accgcgcggg agatcgcctg
     2281 acggatccac cacgaggcgt aggtcgagaa cttgtagccc ttggtgtagt cgaacttctc
     2341 caccgctctg atcaagccga ggttgccctc ctgcacgagg tcgagcagcg gcagcccgcg
     2401 cccggcgtag cgcttggcga tcgagacgac cagccgcagg ttggcctcga cgaggtggga
     2461 cttggcccgc tcgccgtcgg cgcggacggc ctgcagctcg ccggcgatct cggctcgctc
     2521 gccgctggcc agcagctcgg cggcaaacac ccccgcctcg atccggcggg ccagctggac
     2581 ctcttgctca gccgtgagca gggcgacccg gccaatctcg cgcaggtaga cccgcacgag
     2641 gtctgccgac gggccgtcga gcgtgacccg ctcgggtggc tcgtcgtcct ctgcgtcgtc
     2701 gacggcccgg gccgactcgg tgccctcgat ggcctcgccg gcgccggctt cgacgctgac
     2761 cgattcggcc ccgtgctcgg cctcgacctg ggccagctcc tccggcgccg gctcctcgac
     2821 gaggtcaggc gcctgctcgc cgtcgcgccc gtcttcggtg cgcaccacct gcggcaccat
     2881 cccgccctcg gcggtcacca cccgtcgttg gaccaccagc accggcgggg cggcgccagc
     2941 ggagcggtga cgccgcggcg caggcgggga atggacgacg ggccggtcgg gcgacgccac
     3001 ctgcgcagcg tcatctgtgt cgaccgcgcc atgtcgtcga accactcgcg gcctcctcga
     3061 gctctcagtg ctgctgcgct gatcggcaca agtgttaccc gagggcatga tcgaccgcag
     3121 gggttatccg ttgtccatgg cagcagtcgc cggatcagcc ggagccggcg gcccgctcgt
     3181 gcgcgtcgcg ccggcgcgtc tcgagcgcga ccagctcgct gaacaccgcg gcataggcgt
     3241 cggcgtcttc ggtggcgtcg agccgctgca gccgcgagtg caggtcggcc gctcctcgcg
     3301 tgaggtcgag ctcctccagc cgggcgagca gcgcggccac gtagcggccg tcgggcgcgg
     3361 tgtcggcgcg cagcggctcg acggccagct cggtcaccag cgcggccacg ggcgggtccg
     3421 cggccgccgt acggacccga tcgacccagc ccgcctgggc cccggccgct gcgacaccgc
     3481 cgcaggccgc cacgaccgca cggacggcgg cgtaggcggg cgaggtgaag cagctggcgg
     3541 gcagcgcgtc gaacccggcg gcgagcgcgg gcagctgcag gaccgccttg aggctctccc
     3601 gctcgacggc tgtggccggg tcgcgcgggt cgggccgggc gatcgccggg gtcggtggct
     3661 ccggcgccgg gtcgggctgg tgggcgctcg ggtggcccgc cggggccccg gggcgcgtgc
     3721 gggctgcccg gctcacggcg tcgagcaccg gagccacctc catgcccagc cagccggcga
     3781 gccggcgggc gtattcgggg cgcagggacc ggtcgcgaat cgccgccacg atgggggcgg
     3841 ccgaccgcag cgcggccacc cggccctccg ggatctccag gtcgtgcttg cgcagcgtgg
     3901 tccggatgac gaactcgaac agcggcaccc gggcggcgac cagggcgcgt acggcgtcat
     3961 cgccgcggtg ctggcgcagc tcgcacgggt ccatgccgct gggctcgacc gcgacgaacg
     4021 tctgggcgac gaaccgttgg tcgtcggcga acgcccgcag cgcggccttc tgcccggccg
     4081 cgtcgccgtc gaaggtgaag accacctcgc cggccatctc gccgagcgcg tcggtggtgt
     4141 ccatgagcag ccggcggagc accttgatgt gctcttggcc gaaggccgtg ccgcaggtgg
     4201 cgaccgccgt ctgcaccccg gccaggtggc aggccatcac gtcggtgtag ccctcgacga
     4261 tcaccgcctg gtgggtggtc gcgatcggct tgcgggccag gtcgaggcca tagagcacct
     4321 gcgacttctt gtagatcggg gtctcggggg tgttgacgta cttggcggcg atccggtcct
     4381 cctcgagcag cctgcgcgcg ccgaagccca ccacgtcgcc gcggatgtcg cggatcggcc
     4441 agagcagccg gccccggaag tagtcgatcg ggccacgctg gccctggcgc accagcccgg
     4501 cggtgaccag ctcgtcggcg tcgtagccgc ggccccgcag gtgacgggcc agcgcgtccc
     4561 agccggtcgg cgcccagccg accccgaaac gagccgccac ctcgcggtca aagccgcgct
     4621 cggcgaggaa ggcccggccc ggtgcggcct ccgggctggc caacgcctcg gcgtagaact
     4681 cggcggcggc ccggtgcgca gccaccagcc gggcccggcg gcccggatgc agccccgtgg
     4741 gcccgccgcc ctcgtcgtag cgcagtgtga tgccggcctt ggcggcgagc cgctcgaccg
     4801 tctcggcgaa gctgaggtgc tcgagcttca tcacgaacga gatcgcgtcg ccgccctcgc
     4861 cgcagccgaa gcagtggaag ctgccgaccg cggggcgcac cgagaacgac ggggtcttct
     4921 cgtcgtggaa cgggcacagc cccttgaggt ttccgccgcc ggcgttgcgc agggtgacgt
     4981 gctcggcgat gacgtcggcg atgttgaccc gctccttgac gagcgcgacg tcctcgtctc
     5041 ggatccggcc ggccacgccg cggagtctac ggcccgcccc aacccccgcc gcgatcaagc
     5101 aatgtttgcc caccgcggga caacattgct tgatcaccgc ggggtcgggg gcgggccgta
     5161 gactccgcgc cgtggcgggg cggatcaaag acgaggacgt ggcgctggtc aaggagcgcg
     5221 tcaacatcgc cgacgtggtc gccgagcacg tcaccctgcg caacgccggc gggggaaacc
     5281 tcaagggcct gtgcccgttc cacgacgaga agaccccctc gttctcggtg cgtcccagcg
     5341 tcggcagctt tcact
//
LOCUS       DATIBB010000025         2983 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_1062714, whole genome shotgun sequence.
ACCESSION   DATIBB010000025 DATIBB010000000
VERSION     DATIBB010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2983)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2983)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2983
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1062714"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1208)
                     /locus_tag="VLC50_00930"
     CDS             complement(<1..1208)
                     /locus_tag="VLC50_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007512185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSF26056.1"
                     /translation="MTWRERLGALRVDTRPLKESRDFRLLFWAGTVFYLGGMVSYVAL
                     PYQLYTLTGSNFAVGALGIVELVPLVVFGLYGGALADHVDRRTMLVVAGATQVVLTGL
                     LAVNAFLPDPQVWPIYVLGALLAAAQSLQRPSKEALEPRTVRHDQIMAASALSSLGVQ
                     VGLLAGPAVGGLLLASVGVGWCFVVNVVGLLVATALFGALRPYPVTGRSTPPSLAGIR
                     EGLVYAVRRRDLLGTYVVDMVAMFMAMPTVLYPALVEDVFERPELLGLLYSAGTVGSL
                     LATATSGWTRHVHHHGRGVVVAAMAWGAAVAAAGVMPSMWLVLACFVLAGAADMVSGI
                     FRSTVWNQTIPDHMRGRLAGIEMLSYSIGPLGGQARAGLVADAWSVRGSIASGGLLCV
                     AGVAATAAWLR"
     gene            complement(1259..>2983)
                     /locus_tag="VLC50_00935"
     CDS             complement(1259..>2983)
                     /locus_tag="VLC50_00935"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HSF26057.1"
                     /translation="PDEAARHYETALELLGNAANRPAELGDVDLVSLTARASDAMLAS
                     GDLHRALSLVQDQLAHLPADAPVTDRVTLLMTLGLAAIMGDTPVNALEVTSEAMALLP
                     VEPTRLRARLMAAHAWANAEWQRDEEAMRWAQDARELGGRLGLRSVVADATTTIARLE
                     ERAGNPEESKRVFEKIVAQARTDGDVHAELRGLHHLGGLHFEAGQFAEAQRYYSRAVV
                     RAAETNRPWSPYGFDTRVMGAIAAYITGDWDEVARIVDVTGEAPPELIEVSLAAIGLG
                     VAAGRGDADALRTFQRVRPWWDKDGVVAIYSGAAAIDLYADSGDYKSAIRVYEEVVDS
                     VTALWQVSTFLARFRLSGLLLGQLATCGTRLGTTERAELVRTGDRLLDAVRTTRRRAA
                     DRGRRIGPEGLAWTARVEAEHLRLRWLTGVDSPPEDELVGAWRETVAAFTRLGHVFEL
                     ARSRARLSAVLRSLGQTAEARELADQAREVARRLGAEPLLVELRVHGPGRRTDDRARA
                     TEVLTPREQEILALVAEGRSNAEIARQLYISAKTVSVHVSNILAKLGAAGRTEAVAVA
                     RRRGLLAD"
BASE COUNT          421 a         1130 c         1078 g          354 t
ORIGIN      
        1 cgcagccacg ccgccgtcgc cgcgacgccg gccacgcaga gcagcccgcc gctggcgatc
       61 gacccgcgga cgctccacgc gtcggcgacc aaaccggccc gggcctgccc gccgagcggc
      121 ccgatggagt aggacagcat ctcgattccg gcgagccggc cgcgcatgtg gtcggggatc
      181 gtctggttcc acaccgtgga gcggaagatg ccgctgacca tgtcggcggc gccggcgagg
      241 acgaagcagg ccagcaccag ccacatgctc ggcatgaccc cggccgccgc cactgccgca
      301 ccccaggcca tcgccgcgac gaccaccccg cggccgtggt ggtgcacgtg ccgggtccac
      361 ccggaggtgg cggtggcgag caagctgccg accgtccccg cgctgtagag cagcccgagc
      421 agctcgggcc gctcgaacac gtcctcgacg agcgccgggt agagcaccgt cggcatcgcc
      481 atgaacatcg ccaccatgtc caccacgtag gtgccgagca ggtcccgccg gcgcacggcg
      541 tagaccaggc cctcccggat cccggcgagg ctcggcggag tgctccggcc ggtcaccggg
      601 taggggcgca gcgccccgaa cagcgcggtc gccacgagga gaccgaccac gttcaccacg
      661 aagcaccagc cgaccccgac cgaggccagc agcaggccac ccaccgcagg tcccgcgagc
      721 aggccgacct ggacaccgag cgaggacagc gcgctcgccg ccatgatctg gtcgtgccgg
      781 accgtgcgcg gctcgagcgc ctccttcgac gggcgctgca gcgactgggc ggcggcgagc
      841 agcgcgccga gcacgtagat cggccacacc tgcgggtcgg gcaggaacgc gttgaccgcg
      901 agcagcccgg tgagcacgac ctgggtggcg ccggcgacca cgagcatggt ccgacggtcg
      961 acgtggtcgg cgagcgcgcc gccgtacagc ccgaacacca ccagcggcac cagctcgacg
     1021 atgccgagcg cgccgacggc gaagttcgag ccggtcagcg tgtagagctg gtagggcagc
     1081 gcgacgtagc tgaccatgcc gcccaggtag aagaccgtcc ccgcccagaa cagcagccgg
     1141 aagtcgcggg actccttcaa cggccgggtg tccacccgca gcgctcccag gcgctccctc
     1201 cacgtcacgg ccggaagtgt cgcagccgcg gggcgtccgg gcgaccgaaa tacccggttc
     1261 agtccgccag cagcccgcgg cgccgcgcca ccgccaccgc ctcggtgcgg ccggcggcgc
     1321 ccagcttggc caagatgttg gacacgtgca cgctcaccgt cttggcgctg atgtagagct
     1381 gacgggcgat ctcggcgttg ctgcgccctt cggccacgag cgcgaggatc tcctgctcgc
     1441 gcggggtgag cacctcggtg gcgcgtgccc ggtcgtcggt gcgccggccg ggcccgtgca
     1501 cccgtagctc gaccagcagc ggctccgccc cgagcctgcg ggccacctcc cgcgcctggt
     1561 cggcgagctc gcgcgcctcg gcggtctgcc cgagtgagcg gagtacggcg gacagccggg
     1621 cccgggacct ggccagctcg aagacgtggc cgagcctcgt gaacgccgcg acggtctcgc
     1681 gccaggcacc gaccagctcg tcctccgggg ggctgtcgac gccggtgagc cagcgcagcc
     1741 gcaggtgctc ggcctccacc cgcgcggtcc acgccaagcc ctcggggccg atgcgccggc
     1801 cgcggtcggc ggccctgcgt cgggtcgtcc gcaccgcgtc gagcagccgg tcaccggtgc
     1861 gcaccagctc ggcgcgctcg gtggtgccca gccgggtgcc gcacgtcgcg agctggccca
     1921 gcagcagccc gctgagccgg aaccgggcga ggaaggtgga cacctgccac agggcggtga
     1981 ccgagtccac cacctcctcg tagacccgga tcgcgctctt gtagtcacca gagtcggcgt
     2041 agaggtcgat cgccgcggcg ccgctgtaga tcgcgaccac gccgtccttg tcccaccacg
     2101 gccggacccg ttggaaggtg cgcagcgcgt cggcgtcacc gcgcccggcg gccacgccaa
     2161 gaccgatcgc ggccaggctg acctcgatca gctccggcgg cgcctcgccg gtgacgtcga
     2221 cgatgcgggc cacctcgtcc cagtcgccgg tgatgtaggc cgcgatcgcg cccatcacgc
     2281 gggtgtcgaa cccgtagggc gaccacggcc ggttcgtctc cgcggcacgc accaccgcac
     2341 gggagtagta gcgctgcgcc tcggcgaact gaccggcctc gaagtgcagc ccgccgaggt
     2401 ggtgcagccc gcgcagctcg gcgtgcacgt cgccgtcggt gcgcgcctgg gcgacgatct
     2461 tctcgaagac ccgcttggac tcctccgggt tgccggcccg ctcctccagc cgggcgatgg
     2521 tggtggtcgc gtcggcgacg acactgcgca ggccgagccg gccgccgagc tcgcgggcgt
     2581 cctgcgccca gcgcatcgcc tcctcgtcac gctgccactc ggcgttggcc caggcgtgcg
     2641 cggccatcaa ccgggcccgg agcctggtcg gttcgaccgg cagcagggcc atcgcctcgg
     2701 aggtcacctc gagggcgttg acgggggtgt cgcccatgat cgcggccagt cccagggtca
     2761 tcagcagcgt cacgcggtcg gtgaccgggg cgtccgcggg caggtgcgcc agctggtcct
     2821 gcaccagcga cagcgcccgg tgcaggtcgc cggaggcgag catggcgtcg ctggcccggg
     2881 cggtgagcga gaccaggtcg acgtcgccga gctcggccgg ccggttggcc gcgttgccga
     2941 gcagctccag ggcggtctcg tagtggcggg cggcctcgtc ggg
//
LOCUS       DATIBB010000026         3240 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_142029, whole genome shotgun sequence.
ACCESSION   DATIBB010000026 DATIBB010000000
VERSION     DATIBB010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3240
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_142029"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(24..962)
                     /locus_tag="VLC50_00940"
     CDS             complement(24..962)
                     /locus_tag="VLC50_00940"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HSF26058.1"
                     /translation="MPLDPQVRELRDRQGQQGFAAYRSAGIDGARASSRAVIAWRASL
                     APCRDEVATVEDRTIPSAEGTIPARVYLPAGAPTAVVTYLHGGGFVIGDLDASDPHCR
                     AICTRSQAVVVSVDYRLAPEHPFPAPVHDGYAALRWSAETYAGPAGLPLVVAGDSAGG
                     NLAATAAMLARDVGYPRLAAQLLIYPMLDPTMSAPSIVANGEGYLLDAPTLEWFWRHY
                     LGDPPAGALLAAPLTAHLAGLAPALVVTAELDPLRDEGERYAELLAGAGVTTQIQRYD
                     GLVHGFFGWSAFVEAADAAVSQICGQLTDLLQRPAA"
     gene            complement(981..1628)
                     /locus_tag="VLC50_00945"
     CDS             complement(981..1628)
                     /locus_tag="VLC50_00945"
                     /inference="COORDINATES: protein motif:HMM:NF036525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HSF26059.1"
                     /translation="MTTVNGHATEDRVKAAAVQLFGAKGFAATGIRELAHEAGVSIAS
                     LYHYMDTKESLLLDLMLDGMHSLLGPASEALDPPGAPAADLVKLVAIHVRFHCERAAL
                     AKVTDAELRSLSPESRAGIVELRDAYQKLWTDIIIRGRDVGAFKVSEPRLATLALLEM
                     CTGVYHWYSPGGRFTVDRIADYYGVLALRMLAHPRTPAMRRLEAAVVVDSAPGSS"
     gene            complement(1655..2173)
                     /locus_tag="VLC50_00950"
     CDS             complement(1655..2173)
                     /locus_tag="VLC50_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016701258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HSF26060.1"
                     /translation="MPIYSLDGVCPEIHPSAFVHPDAVVIGNVTIHAEASVWPCAVLR
                     GDYGRIIIGERTSVQDGSIIHATEEHPTIVGSECVIGHSVHMEGCHVIAQCLIGSNST
                     VLHAVTIEPGSVVAAGAVVKANTHVPSNSLVAGVPARIVAQDRDYEQFRSTVDQYVAN
                     ARRYETSLQLLD"
     gene            complement(2154..>3240)
                     /locus_tag="VLC50_00955"
     CDS             complement(2154..>3240)
                     /locus_tag="VLC50_00955"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR00045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HSF26061.1"
                     /translation="DVRAIPMSDGGPGFVDAVLTIPEAHPHTVVVPGPRGRSVPARIA
                     VHRKVAYVESAMACGAQALGPDPLDPLASGTHGVGLLVRACCELPVDTVVVGLGGSVT
                     NDGGMGALSALGAVFRDEVGRTLPGCGADLARVASVDLSGVPAALSSRVQLVLAADVD
                     SALLGATGASRGFGPQKGADREMVGRLESGMGLLAEVLERAAGRGRLREAPGSGAAGG
                     LGFGLMALGASRRPGFDVVAELVELTAGIGSADLVLTGEGRLDWQSLHGKVVHGVATA
                     CAKQARPCWVIAGAIDDPDRVVAELGLAGASSLTEHAGSSARARAEAAVVLEQLSSQV
                     AATTRPGAGGLVRPRREERPERAHLQP"
BASE COUNT          502 a         1150 c         1156 g          432 t
ORIGIN      
        1 tgatcagtgc agggtggaga ggctcaggcg gcgggacgct gcagcaggtc ggtgagctgg
       61 ccgcagatct gcgacaccgc ggcgtcggcg gcctcgacga acgcgctcca gccgaagaag
      121 ccgtgcacca gcccgtcgta ccgctggatc tgggtggtca ccccggcccc ggcgagcagc
      181 tcggcatatc gctcgccctc gtcgcgcagc gggtcgagct cggcggtgac cacgagcgcc
      241 ggtgccagcc cggcaaggtg ggccgtcagc ggtgccgcca gcagtgcgcc tgcgggcggg
      301 tcgccgaggt agtggcgcca gaaccactcg agcgtggggg cgtcgagcag gtagccctcg
      361 ccattggcga cgatcgacgg cgccgacatc gtggggtcga gcatcgggta gatcagcagc
      421 tgggcggcca gccgcgggta accgacgtcg cgcgccagca tcgccgcggt cgcggccagg
      481 ttgccgcccg cgctgtcccc agccacaacc agcggcaggc cggcagggcc ggcgtaggtc
      541 tcggcgctcc agcgcagcgc ggcgtagccg tcgtgcaccg gagccgggaa cgggtgctcg
      601 ggggccagcc ggtagtcgac gctgacgacc acggcctgcg accgcgtgca gatggcccgg
      661 cagtgggggt cgctcgcgtc gagatcgccg atgacgaaac cgccgccgtg cagataggtg
      721 accacggcgg tcggtgcccc ggccgggaga tagacccggg cagggatcgt gccctcggcc
      781 gaagggatcg tgcggtcctc gaccgtggcc acctcgtcgc ggcagggggc cagcgaggcc
      841 ctccaggcga tgaccgcacg agacgaggcc cgggccccgt cgatcccggc gctgcgatag
      901 gcggcgaacc cctgctggcc ctggcggtcg cgcagctcgc gcacttgcgg atcaaggggc
      961 acgacggtct cctgggacgg tcagctcgag ccgggcgcgg agtccacaac gacggcggcc
     1021 tcgagccggc gcatggccgg cgtccgaggg tgcgcgagca tccggagcgc cagcacgccg
     1081 taatagtcgg cgatgcggtc aacggtgaag cggccacctg gggagtacca gtggtagacc
     1141 ccggtgcaca tctcaagcag cgccagcgtg gccagccgcg gctcgctgac cttgaatgcg
     1201 ccgacgtccc tgcccctgat gatgatgtcg gtccacagct tctggtaggc gtcgcgcagc
     1261 tcgacgatgc ccgcccgcga ctccggcgag agcgagcgca gctcggcgtc ggtgaccttg
     1321 gccagcgctg cccgctcgca gtggaacctg acgtggatcg cgaccagctt gaccaggtcg
     1381 gcggcgggcg cacccggcgg gtcgagcgcc tcggatgccg ggccgagcag gctgtgcatg
     1441 ccatcgagca tcaggtcgag aagcaggctc tccttggtgt ccatgtagtg atagagcgac
     1501 gcgatggaga cgccggcctc gtgcgcgagc tcgcgaattc ccgtcgccgc gaatcccttg
     1561 gcaccaaaca attgcaccgc ggcggccttc acccggtctt cggtggcatg cccgttaacc
     1621 gtggtcatgg gattctggac tccgtccgtc gagctcagtc gagcagctgt agcgacgtct
     1681 catagcgtcg agcgttggcc acatattggt cgaccgtgct gcggaactgc tcgtagtcac
     1741 ggtcttgcgc cacgatgcgg gcagggaccc ccgcaacgag cgaattcgat ggtacatggg
     1801 tgtttgcctt gaccaccgca ccggccgcca cgaccgagcc gggctcgatc gtgaccgcgt
     1861 gcagcacggt ggagttggac ccgatgaggc actgcgcaat gacgtggcag ccctccatgt
     1921 gcacggaatg cccgatgaca cattccgatc cgacgatggt cgggtgctcc tcggtcgcgt
     1981 gaatgatcga cccgtcctgc accgaggtgc gctccccgat gatgatgcgc ccgtagtcgc
     2041 cgcgcagcac ggcgcacggc cacaccgagg cctccgcatg gatcgtcacg ttgccgatca
     2101 ccaccgcatc gggatgcacg aaggcgctcg ggtggatctc ggggcagacc ccgtcaaggc
     2161 tgtagatggg cacgctcagg cctttcttcg cgtcgtggtc tcaccaggcc cccggcacca
     2221 ggccgggtgg tcgccgccac ctgtgaggag agctgttcga ggaccaccgc ggcctccgcg
     2281 cgggcccgag ctgatgagcc ggcgtgctcg gtgagcgagc tggcgccggc caggccgagc
     2341 tccgccacga cccggtcggg atcgtcgatc gcgcccgcga tgacccagca tggacgcgcc
     2401 tgcttcgcgc atgccgtcgc caccccgtgc accaccttgc cgtgaagtga ctgccagtcg
     2461 aggcggccct caccggtgag cacgaggtct gcggagccga tcccggcggt cagctcgacg
     2521 agctcggcca ccacgtcaaa gccggggcgg cgagacgcac ccaaggccat cagcccgaag
     2581 ccgaggccgc cagcagcgcc gctgccgggc gcctcgcgca accgtccacg gccggctgcc
     2641 cgctcgagca cctcggccaa cagacccatg cccgactcga gccggccaac catctcgcgg
     2701 tcggcgccct tctgagggcc gaagccgcgg ctggctccgg tcgcgcccag cagtgcgctg
     2761 tcgacatcgg ccgccaggac gagctggacc cgagacgaca gcgcggcggg gacacccgac
     2821 aggtcgacgg aggcgacccg ggccaggtca gccccgcacc ccggaagcgt tctgccgact
     2881 tcgtcgcgaa agaccgcgcc gagcgccgac aaggccccca tgccgccgtc gttggtgacc
     2941 gacccgccca ggcccaccac caccgtgtcc accggcagct cgcaacaggc tcggaccagc
     3001 aggccgaccc cgtgggtgcc gctcgccagc gggtcgagag ggtcggggcc cagggcctgc
     3061 gcgccgcagg ccatcgccga ctcgacgtag gccaccttgc ggtgcaccgc tatccgggcc
     3121 ggcacggacc gaccccgcgg cccgggcacg acgacggtgt gcggatgcgc ctcggggatg
     3181 gtgagcaccg cgtcgacgaa tcctgggccg ccatcgctca tggggatggc gcgcacgtcg
//
LOCUS       DATIBB010000027         4810 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_1062840, whole genome shotgun sequence.
ACCESSION   DATIBB010000027 DATIBB010000000
VERSION     DATIBB010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 4810)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4810)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4810
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1062840"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..565
                     /locus_tag="VLC50_00960"
     CDS             <1..565
                     /locus_tag="VLC50_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012879050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HSF26062.1"
                     /translation="VVHSVTKYLAGHSDAVLGATVTTDPAHLDRLRRVRSLRGAVPGP
                     VEAWLALRGLRSLHVRLDRAQANAGELARRLAAHPGVDRVRYPGLPTDPGHALATAQM
                     QGYGAIVALEVAGGAEAADAVCEAVRLWAVATSLGSVESTLERRRRWPAESPDIPPAL
                     LRLSVGIEDVEDLWDDLDAALRAPLGG"
     gene            complement(569..1570)
                     /locus_tag="VLC50_00965"
     CDS             complement(569..1570)
                     /locus_tag="VLC50_00965"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016909911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HSF26063.1"
                     /translation="MTRVAVLGAGSWGTAFAMVLSDAGADVVLWARRTELAEAITREH
                     QNPDYHPGIELPPALWATSEPAEALRERDLVVLAVPSQVLRGNLAAWAPLLPAGAPVV
                     SLMKGVEVGTAKRMSEVVCEVAGLPPERVVVVSGPNLSREIVTRQPSASVIACVDESA
                     AVAFQQACMTPYFRPYTDTDVVGVELGGAVKNVIALACGMAEGMGYGDNTRATIITRG
                     LAETMRLGLVLGADPQTFAGLAGLGDLVATCTSRLSRNRTFGERIGRGESVAEIVAST
                     RQVAEGVTTCESVLALARAHAVDVPITQAVVDVVRDGMPVREMAGRLMGRAAKPEKV"
     gene            complement(1567..2343)
                     /locus_tag="VLC50_00970"
     CDS             complement(1567..2343)
                     /locus_tag="VLC50_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HSF26064.1"
                     /translation="MQRRPRRRSEPRGFWYAAAAILLRPVLFVTTRPQWQGGEHLPAH
                     GGVVVCPNHVSYVDPLTFAHFVYDHGRCARFIAKASLFEIPGLRRLLIGAGQIPVYRE
                     SGRAGQALSAAVDAVRAGRCVAVYPEATITRDDDLWPMAGKTGAARIALATGAPVIPV
                     AQWGPQEILPPYAKRPRLFPRHTVHVHAGPPVDLSEFAGRELTAEVLTQATEKIMAAI
                     TELLAAIRGQRPPAVRFDPRTSDLPRTGDPRRRGDHGAAR"
     gene            complement(2430..3017)
                     /locus_tag="VLC50_00975"
     CDS             complement(2430..3017)
                     /locus_tag="VLC50_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HSF26065.1"
                     /translation="MNKAQLVEELSKAFEGNRRQASNALQTVIDTITRAVAKGEKVAI
                     TGFGAFERVERAARTARNPRTGAAVKLKKTAVPKFRAGASFKEVVADPRKLPKQAKPA
                     KAAKAAPAKAAAKTAKTAKAAPAKAAKAPAKATKAAKAAPAKATKATKTAKATKAAPA
                     AKTATRATAKATKAPAKKTTAKAAKAPAKKATKRS"
     gene            complement(3336..3935)
                     /gene="leuD"
                     /locus_tag="VLC50_00980"
     CDS             complement(3336..3935)
                     /gene="leuD"
                     /locus_tag="VLC50_00980"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013807978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HSF26066.1"
                     /translation="MPPFTVHTGRVLPLRRSNVDTDQIIPAVYLKRVTRTGFEDGLFA
                     AWRQDPGFVLNRPEYAGATVLVAGPDFGTGSSREHAVWALRDYGFRVVISPRFADIFR
                     GNSGKAGLLTAVVDLSVVERLWALAESDPATELTVDLQAREIRAGELVAPFDIDDYTR
                     WRLLEGLDDIDLTLRHADLVQAFEAGRPGWMPTTLPARG"
     gene            complement(3991..>4810)
                     /locus_tag="VLC50_00985"
     CDS             complement(3991..>4810)
                     /locus_tag="VLC50_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018171127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HSF26067.1"
                     /translation="VIEYRGEAVRSLSMEGRMTVCNMSIEAGARAGMIAPDETTFAYL
                     QGRPHVPSGTDWDEAVAYWQTLHTDDDAVYDREVVIEASELAPFVTWGTNPGQGVALN
                     GQVPDPAELDDPGARAAAERALTYMDLQPGTPMKDIAVDAVFIGSCTNGRIEDLRAAA
                     AVLRGRQVAAGVRMLVVPGSAKVRRQAEAEGLDQVFVQSGAEWRQPGCSMCLGMNPDQ
                     LAAGERCASTSNRNFEGRQGKGGRTHLVSPVVAAATAVTGRLASPADLAELQPA"
BASE COUNT          624 a         1731 c         1761 g          694 t
ORIGIN      
        1 cgtggtccac agcgtcacga agtacctcgc cgggcacagc gacgcggtgc tgggcgccac
       61 ggtcacgacc gacccggccc accttgaccg cctgcggcgg gtccggagcc tgcgcggggc
      121 ggtgccggga ccggtcgagg cgtggctggc gctgcggggg ctgcgctcgc tgcacgtgcg
      181 cctcgatcgc gcgcaggcca acgccggcga gctcgcccgt cggctcgcgg ctcatcccgg
      241 ggtcgaccgg gtccgctacc ccggcctgcc caccgacccc ggccatgcgc tggccacggc
      301 tcagatgcag gggtacggcg cgatcgtcgc gctcgaggtg gccggaggcg ccgaggccgc
      361 cgacgcggtc tgcgaggcgg tcaggctctg ggcggtggcc accagcctcg gctcggtcga
      421 gtcgacgctg gaacgtcgcc ggcgctggcc ggctgagagc cccgacatcc cgccggcgct
      481 gctgcggctg tccgtcggga tcgaggacgt cgaggacctc tgggacgacc tcgacgccgc
      541 cctgcgcgcc ccgctgggcg gctgaggctc agaccttctc cggcttggct gcccgcccca
      601 tgagccggcc cgccatctca cgcaccggca tcccgtcgcg gaccacgtcg acgaccgcct
      661 gggtgatcgg cacatcgacg gcgtgcgcgc gggccagcgc gagcaccgac tcacaggtcg
      721 tcacgccctc ggcgacctgc cgggtcgagg ccacgatctc ggcgaccgac tcgccgcgcc
      781 cgatccgctc gccgaacgtg cggttgcggg acaaccggga ggtgcaggtg gccaccaggt
      841 cgccgagccc ggccagcccg gcgaacgtct gcgggtcggc gcccaggacc aggcccaggc
      901 gcatggtctc ggccagcccc ctcgtgatga tcgtcgcccg cgtgttgtcg ccgtagccca
      961 tgccctcggc catgccgcag gccagcgcga tgacgttctt gaccgccccg ccgagctcga
     1021 cgccgacgac gtcggtgtcg gtgtagggcc ggaagtaggg cgtcatgcag gcctgctgga
     1081 acgccaccgc cgccgactcg tcgacgcagg cgatgacgct ggccgacggc tgacgggtca
     1141 cgatctcccg cgacaggttg ggcccggaca cgacgaccac gcgctcggga ggcagcccgg
     1201 cgacctcgca gaccacctcg ctcatccgct tggcggtccc gacctcgacg cccttcatga
     1261 ggctgacgac cggcgcaccg gccggcagca gcggtgccca cgccgcgagg ttgccgcgca
     1321 gcacctgcga cggcaccgcc agcaccacca ggtcgcgctc gcgcagcgcc tcggccggct
     1381 cgctggtcgc ccacagcgcg ggcggcagct cgatgccggg gtggtagtcg gggttctggt
     1441 gctcacgggt gatcgcctcg gccagctcgg tccggcgggc ccagagcacg acatcggctc
     1501 cggcgtcgct gagcaccatg gcaaaggcgg ttccccagga tccagccccg aggacggcga
     1561 cgcgggtcat cgggcggctc cgtggtcgcc tcggcggcgc gggtcgccgg tgcggggcag
     1621 gtctgaggtg cgcgggtcga agcggacggc aggcggtcgc tggccccgga tcgccgcgag
     1681 cagctcggtg atcgccgcca tgatcttctc cgtcgcctgt gtgagcacct cggccgtgag
     1741 ctctctcccg gcgaactcgc tcaggtcgac cggcggtccc gcgtgcacgt gcacggtgtg
     1801 acgcgggaac aggcggggac gcttggcata gggcgggagg atctcctgcg ggccccactg
     1861 tgccacgggg atcaccggcg caccggtggc cagggcgatc cgcgccgcgc cggtcttgcc
     1921 ggccatcggc cacaggtcgt cgtcgcgggt gatcgtcgcc tccgggtaga ccgcgacgca
     1981 ccggccggcc cggacggcgt ccaccgccgc cgagagcgcc tggccggccc ggccgctctc
     2041 gcggtagacc gggatctgac cggcgccgat cagcagccga cgcaggccgg ggatctcgaa
     2101 gagcgaggcc ttggcgatga agcgggcaca ccgcccgtgg tcgtagacga agtgcgcgaa
     2161 cgtcaacggg tcgacgtagg acacgtggtt ggggcagacc acgacgccgc cgtgggccgg
     2221 caggtgctca ccgccctgcc actgcgggcg ggtcgtgacg aagagcaccg gccgcagcag
     2281 gatggccgcc gcggcgtacc agaacccacg aggctcgctc cgtcggcggg ggcgccgctg
     2341 caccgcgctg tcctccaagt cgtcgagatg ctggtgacgc gacgaggggc cgaacccacg
     2401 cgggtccagc ccctcgtcgt tcgcggtggt caggagcgct tggtcgcctt cttggccggc
     2461 gccttggccg ccttggcggt ggtcttcttc gccggcgcct tggtggcctt ggcggtcgcc
     2521 cgcgtggccg tcttggcggc gggcgcggcc ttggtggcct tggccgtctt cgtggccttg
     2581 gtggccttgg ccggagcagc cttggcggcc ttggtcgcct tggcgggggc cttggcggcc
     2641 ttggccggag cagccttcgc cgtcttggcg gtcttggcgg ctgccttggc cggagcagcc
     2701 ttggcggcct tggccggctt ggcctgcttg ggcagcttgc gcgggtcggc cacgacctcc
     2761 ttgaaggacg cgccggcacg gaacttcggc accgcggtct tcttgagctt gaccgccgcc
     2821 ccggtgcgcg ggttgcgggc agtccgagcc gcccgctcga cacgctcgaa cgccccgaag
     2881 ccggtgatgg ccaccttctc gcccttggcc acggcccggg tgatcgtgtc gatcaccgtc
     2941 tggagtgcat tggaggcctg ccgcctgttg ccctcgaagg ccttggagag ctcctcgacc
     3001 agttgagcct tgttcactgc ttcccctccg gaaactggcg tcgagccttc gcccgactct
     3061 gcgcgaacgg taggccgatg cggcgcgacg cacaatcacc atcagagtga tttccgttgt
     3121 gtcgccacgg gttccgagac ttggctacct ctcacctcgg ccgcgggcaa gggctgtcgt
     3181 ccggcacgtg acgcaccatc agggacacga cgcggctcgg cgatcacccg acgggtgcgt
     3241 gaaacacgtt gcaacgcaag ggattacgcc gacctcacgg cgtcggtcac gtgccgcggc
     3301 gacctcagtg gcgagacggg ccgccgaggc gatcgtcagc cgcgcgccgg cagcgtcgtc
     3361 ggcatccacc cgggtcggcc cgcctcgaaa gcctgcacga gatcggcgtg gcgaagcgtc
     3421 aagtcgatgt cgtcgaggcc ctcgagcagg cgccaacgcg tgtagtcgtc gatgtcgaag
     3481 ggcgccacga gctcgccggc acggatctcg cgggcctgca ggtccacggt cagctcggtg
     3541 gccgggtcgc tctcggcgag cgcccacagc cgctcgacca ccgacaggtc aacgaccgcg
     3601 gtgagcaggc ccgccttgcc ggagttgccg cgaaagatgt cggcgaagcg gggagagatc
     3661 acgacccgga agccgtagtc gcgcagcgcc cagacggcgt gctcccgcga ggagccggtg
     3721 ccgaagtccg gtccggcgac gagcaccgtg gcaccggcgt actccggacg gttgagcacg
     3781 aagccgggat cctggcgcca ggcggcgaag agcccgtcct cgaacccggt ccgcgtgacc
     3841 cgcttgaggt agaccgccgg gatgatctgg tcggtgtcga cgttgctgcg ccgcaacgga
     3901 aggacccgtc ccgtgtgcac ggtgaagggc ggcatcgcgg tcacgttcat cagtcctctc
     3961 gagcgctgcg gtctggcggt gtggtggtgc tcaggcgggt tggagctcgg cgagatcggc
     4021 gggtgaggcc aaccggccgg tcaccgcggt ggccgcagcc acgacgggcg agacgaggtg
     4081 ggtgcgcccg cccttgccct ggcgtccctc gaagttgcgg tttgacgtgg aggcgcaccg
     4141 ctccccggcc gccagctggt cggggttcat gcccaggcac atcgagcacc cgggctgacg
     4201 ccactcggcg ccggactgca cgaagacctg gtcgaggcct tcggcctcgg cctggcgccg
     4261 gaccttcgcc gagccgggta cgacgagcat ccggaccccg gcggcgacct gccggccgcg
     4321 cagcaccgcc gccgccgccc gcaggtcctc gatgcgcccg ttggtgcagg agccgatgaa
     4381 gaccgcgtcg accgcgatgt ccttcatcgg ggtgcccggc tgcaggtcca tgtaggtcaa
     4441 ggcccgttcg gccgcggccc gggcacccgg gtcgtcgagc tcagccgggt caggcacctg
     4501 gccattgagc gcgaccccct ggccggggtt ggtcccccag gtgacgaagg gcgccagctc
     4561 gctggcctcg atgacgacct cgcggtcgta gaccgcgtcg tcgtcggtgt gcaacgtctg
     4621 ccaataggcg accgcctcgt cccagtcggt gccgctgggg acgtgcggtc ggccctgcag
     4681 ataggcgaac gtcgtctcgt cgggggcgat catgccggcc cgggcccccg cctcgatcga
     4741 catgttgcac acggtcatgc ggccctccat cgacagggac cgcaccgcct cgccgcggta
     4801 ctcgatgacg
//
LOCUS       DATIBB010000028         7388 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_12120, whole genome shotgun sequence.
ACCESSION   DATIBB010000028 DATIBB010000000
VERSION     DATIBB010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 7388)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7388)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7388
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12120"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..589)
                     /locus_tag="VLC50_00990"
     CDS             complement(<1..589)
                     /locus_tag="VLC50_00990"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF21 domain-containing protein"
                     /protein_id="HSF26068.1"
                     /translation="MSGSTHVGLLVVAVVLVVLAGVFAAGESALSRLSRTRADELVRD
                     GRRHAGTLQAVLADPGRYVNLLTFLRVVAEVVATVLVTLVCLDVLPRLRALALASVVM
                     VVVSYVAIGVSPRTVGRQHADRVGLLTAGVVHPLARVLGPIPALLILVGNALTPGRAL
                     PDGPFATEAELRDLVDLAQENRLIEADERAMIHSVF"
     gene            complement(586..1074)
                     /gene="ybeY"
                     /locus_tag="VLC50_00995"
     CDS             complement(586..1074)
                     /gene="ybeY"
                     /locus_tag="VLC50_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020574570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HSF26069.1"
                     /translation="MSIDINNESGVEVDLDAVVVLARHVLDRMRIHPLAELSVLLVDE
                     AAMTELHVRWMDEPGPTDVLSFPMDELRPGRDDEEPEPGLLGDVVLCPQVAARQAVEA
                     GHSMQDELLLLTTHGILHLLGYDHAEPDEEREMFGLQRELLGEWARVQPGSPGPSGGE
                     DG"
     gene            complement(1084..2142)
                     /locus_tag="VLC50_01000"
     CDS             complement(1084..2142)
                     /locus_tag="VLC50_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020553173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HSF26070.1"
                     /translation="MAEPRQAQTTIVVPASQPMVALLGPADELLRVIEAAFPDTDIHV
                     RGNEISVTGDPAEVALVERLVEELILVLRTGQGLTAEAVARSVAMLRAQTTERPADVL
                     TLGILSSRGRTIRPKTLNQKRYVDAIDRHTITFGIGPAGTGKTYLAVAKAVQALQAKA
                     VTRIVLTRPAVEAGERLGFLPGTLSEKIDPYLRPLYDALHDMVDPASIPRLMTEGTIE
                     VAPLAYMRGRTLNDAFIILDEAQNTSAEQMKMFLTRLGFGSRMVVTGDVTQVDLPGGT
                     QSGLRVVQEILDGVDDVAFCRLTSSDVVRHRLVSDIVEAYGRYDASRGKDERGRSAVA
                     GPPPRGSAQRGSAQRGRG"
     gene            complement(2228..3631)
                     /locus_tag="VLC50_01005"
     CDS             complement(2228..3631)
                     /locus_tag="VLC50_01005"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013735390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HSF26071.1"
                     /translation="MAPGLMAARSPADTSDTCATARPLPDELARRLALMTADRQKAWR
                     SPAVAAAVVRDGEPVWSHEVGLADVESETAPTPDTQFRMGSITKTFTATLVMQCRDEG
                     LLDLDDRLEAHLPGTGFGDLTLRRMLGHVSGLQREPAGEIWSRPEGPTRAELLAGLED
                     AEAVLASGRRWHYSNLAFALLGELVARRRGQPWEEVLSERILGPLGMRRTTTRRTAPH
                     ATGYLSDPYADRVRPEPEFPGNAFAPAAELWTTTTDLGRWGAFLADPDAVGPGVVSAD
                     TVEEMCHPQVIADLDAWTLGWGLGLMLVRRGDRVLVGHGGAMPGFLAGLLVRRPEKVA
                     AVVLANTSAGADASGLAADLVLEVVDADPAPPAPWRPGPPVPAEVEALLGVWWTEGNQ
                     VIFSWHDGRLEARLAAAPAEKPPAVFVADGYDRFRVESGREHGELLRVVRRDDGSVDR
                     MVWATYPVTRDCLPFGG"
     gene            complement(3616..3972)
                     /locus_tag="VLC50_01010"
     CDS             complement(3616..3972)
                     /locus_tag="VLC50_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019877917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HSF26072.1"
                     /translation="MSETRRDDDCVFCKIVAGEIPATLVRESERTLAFRDLNPIAPTH
                     VLVITRDHHGTVGAVAEADPEALVELVRECQAVAAQEGLPGYRLVFNSGAAAQQTVDH
                     VHGHVIGGRAVTWPPG"
     gene            4126..4635
                     /locus_tag="VLC50_01015"
     CDS             4126..4635
                     /locus_tag="VLC50_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017620752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SigE family RNA polymerase sigma factor"
                     /protein_id="HSF26073.1"
                     /translation="MTSSTTWDADEAVTALYTAHYRSLVRLSALLLREQGAAEEVVQD
                     AFVAMHGAWRRLRDPDKSLAYLRRTVVNRSRSALRRRRTAEKYAPAPMPDAASAEHHA
                     LVALEHQAVVRALHTLPTRQRETLVLRYYADLSEAEIAEAMGISRGAVKSHASRGMAT
                     LRSTLEQQS"
     gene            4632..5675
                     /locus_tag="VLC50_01020"
     CDS             4632..5675
                     /locus_tag="VLC50_01020"
                     /inference="COORDINATES: protein motif:HMM:NF022112.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gmad2 immunoglobulin-like domain-containing
                     protein"
                     /protein_id="HSF26074.1"
                     /translation="MTYDDDELAARLRRALHAEAEMVNPRGDGLSQIRGRTAGRGSPG
                     LMRWAVGAAAAVVIAAVGVGAGVMWSNQSDGQGAIIASTPSGATSAPAPTPSESGSAS
                     PLPGTRVTVPVYWLGDAADRPGLYREFASVLAVDASTLSKVQVAVSQALFGTPADPDY
                     STPWAKGSVATVTLGADEIGIDLNKAATDGSSMGSQIAQLAVQQLVWTATAAAGENLP
                     VRITVEGATPDLFGTVSLAEPIRRADPAYEALANIWILTPTQGQAVSGAVTATGQATV
                     HEANLLWQLLKGSTVVRNDFTTASEGGPGRGDWTIELGTLDPGTYTLRAYAESPKGDG
                     SLQGEDTKTFTVK"
     gene            complement(5679..6425)
                     /locus_tag="VLC50_01025"
     CDS             complement(5679..6425)
                     /locus_tag="VLC50_01025"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008748001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HSF26075.1"
                     /translation="MSAPVFVADAATLAEAVAGARIRLTGPEGHHAATVRRLRPGEPV
                     VVTDGTGRAAACRVAAVMGRDVVELDVDTVRDEPAPAPRLVVVQALAKGEHGELAVDL
                     MTQVGVDVIVPWAASRSVVQWRGDKLAKGRARWRATAHEAAKQARRARFPEVTEPAST
                     AEVVALLQIAACALVLHESAEQPLAGLALPDAGDVVVVVGPEGGIAPDELDAFVGAGA
                     VVVRLGETVLRTSSAGAAALAVASAATRWR"
     gene            complement(6436..>7388)
                     /locus_tag="VLC50_01030"
     CDS             complement(6436..>7388)
                     /locus_tag="VLC50_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018846694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HSF26076.1"
                     /translation="SDPRKRELYDVGADPDGGHGGFGAGFGFSDIMDAFFGASAARGP
                     RPRARRGQDALIRLDLDLSEAVFGASKELQVDTAVVCQACHGDGAAPGTAPRTCEVCQ
                     GRGEVQHVTRSFLGQVMTSRPCVACQGYGTVISTPCAECGGDGRVRARRSLAIRIPAG
                     VDTGTRIQLSGEGEVGPGGGAPGDLYVEVVVRPHELFQRHGDDLYCTVSLPMTAAALG
                     TSVELETLDGIETIDIRPGTQSGQTIPLHGLGVTHLRGAGRGDLIVHVEVTTPTKLDA
                     EQEELLRKLAAARGEERPAGQFSPGQQGLFSRLRDAFNGR"
BASE COUNT         1008 a         2695 c         2758 g          927 t
ORIGIN      
        1 cgaacaccga gtggatcatc gcgcgctcgt cggcctcgat gagccggttc tcctgggcca
       61 ggtcgaccag gtcgcgcagc tctgcctcgg tggcgaacgg cccgtcgggg agggctcggc
      121 ccggggtcag cgcgttgccg acgaggatga gcagcgccgg gatcggtccg aggacgcggg
      181 ccagcgggtg gacgacgccc gcggtgagca ggccgacacg gtcggcgtgc tggcgcccga
      241 cggtgcgcgg ggagacgccg atcgcgacgt aggagacgac gaccatgacc accgacgcga
      301 gtgccagcgc gcgcaggcgg ggcagcacgt cgaggcagac aagagtgacg agcacggtcg
      361 cgacgacctc ggcgaccacc cgcaggaacg tcagcaggtt gacgtagcga ccgggatcgg
      421 cgagcacggc ctgcagcgtg ccggcgtgcc ggcgcccgtc acggacgagc tcgtcggccc
      481 gcgtgcggga cagccgcgag agcgcggact caccggcggc gaacaccccg gccagcacga
      541 ccaggactac ggcgaccacg agcaggccga cgtgggtgct cccgctcacc cgtcttcgcc
      601 tcccgacggg ccgggcgacc cgggctgcac gcgggcccac tcgccgagca gctcgcgctg
      661 cagcccgaac atctcgcgct cctcgtcggg ctcggcgtgg tcgtagccca gcaggtgcaa
      721 gatcccgtgg gtggtgagca ggagcagctc gtcctgcatg ctgtgcccgg cctcgacggc
      781 ctgccgggcc gcgacctgcg ggcagagcac gacgtcaccg agcagcccgg gctcgggctc
      841 ctcgtcgtcg cggcccggcc gcagctcgtc catcgggaag gacagcacgt cggtggggcc
      901 gggctcgtcc atccatcgca cgtgcagctc ggtcatcgcg gcctcgtcga ccagcagcac
      961 cgacagctcg gcgagggggt ggatgcgcat ccggtcgagc acgtgccgtg ccagcacgac
     1021 caccgcgtcg aggtcgacct cgacccccga ctcgttgttg atgtcgatgc tcatcgggtg
     1081 cccctagccc cggccacgct gggccgaccc acgctgggcc gacccgcgcg gtggcggccc
     1141 ggccacggcc gaccggcccc gctcgtcctt gccccggctg gcgtcgtagc gcccgtaggc
     1201 ctcgacgatg tcggaaacca gccggtggcg tacgacgtcg ctgctggtca ggcggcagaa
     1261 cgcgacgtcg tcgacgccgt cgaggatctc ctgcacgacg cgcagcccgc tctgggtgcc
     1321 gccgggcagg tcgacctggg tgacgtcgcc ggtgacgacc atgcgggagc cgaagccgag
     1381 tcgggtcagg aacatcttca tctgctcggc cgaggtgttc tgcgcctcgt cgaggatgat
     1441 gaacgcgtcg ttgagggtgc gcccgcgcat gtaggccagc ggagccacct cgatggtgcc
     1501 ctcggtcatc agccgcggga tcgacgccgg gtcgaccatg tcgtgcagcg cgtcgtagag
     1561 cgggcgcagg taggggtcga tcttctcgct gagggtgccg ggcaggaacc cgagccgctc
     1621 gcccgcctcg accgccgggc gggtcagcac gatccgggtg accgccttgg cctgcagcgc
     1681 ctgcacggcc ttggccacgg cgaggtaggt cttgccggtg ccggccggcc cgatgccgaa
     1741 cgtgatggtg tgccggtcga tcgcgtcgac gtagcgcttc tggttgagcg tcttgggccg
     1801 gatcgtgcgg ccccggctgg acaggatgcc cagggtgagc acgtcggccg gccgctcggt
     1861 ggtctgggcc cgcagcatcg ccaccgagcg ggcgaccgcc tcggcggtga gcccctggcc
     1921 ggtgcgcagc acgaggatga gctcttcgac gaggcgctcg acgagggcga cctcggccgg
     1981 gtcaccggtc acggagatct cgttgccccg cacgtggatg tcggtgtcgg ggaacgctgc
     2041 ctcgatgacc cggagcagct cgtcagccgg gccgagcaga gcgaccatcg gctggctcgc
     2101 gggtacgacg atcgtggtct gggcttgtcg tggttcggcc atgggtgccc cgaccggggc
     2161 ctgcactcct taggacggac ggtgggcacc catgctaccc agggcggggc ggcaccccga
     2221 cggcatttca gccgccaaaa ggcaggcagt cgcgggtcac cgggtaggtg gcccagacca
     2281 tccggtcgac gctgccgtcg tcgcgccgta cgacgcgcag cagctcgccg tgctcgcgac
     2341 cggattcgac ccggaaccgg tcatatccgt ccgcgacgaa caccgccggc ggcttctctg
     2401 ccggcgcggc cgccagccgt gcctcgagcc ggccgtcgtg ccaggagaag atcacctggt
     2461 tgccctcggt ccaccacacg cccagcagcg cctcgacctc tgccggcacc gggggccctg
     2521 gccgccacgg ggcgggcggg gccgggtcgg cgtcgacgac ctcgaggacc aggtcggcgg
     2581 ccagcccgga cgcgtcggcg ccggccgagg tgttggccag caccacggcc gcgaccttct
     2641 cgggccgccg cacgagcagc ccggccagga accccggcat cgcgccgcca tggccgacca
     2701 ggactcggtc accgcggcgg accagcatca ggcccagccc ccagcccaac gtccaggcgt
     2761 cgaggtcggc gatcacctgc gggtggcaca tctcctcgac cgtgtcggcc gagacgacgc
     2821 cgggcccgac ggcgtcgggg tcggcgagga acgcgcccca gcggcccagg tcggtcgtgg
     2881 tggtccacag ctcggcggcc ggcgcgaacg cgttgccggg gaactccggt tccgggcgca
     2941 cccggtcggc gtaggggtcg gacaggtagc cggtcgcgtg cggcgcggtg cggcgggtcg
     3001 tcgtacggcg catcccgagc ggcccgagga tgcgctcgga caggacctcc tcccacggct
     3061 ggccgcgacg gcgggccacc agctcgccga gcagggcgaa agcaaggttg gagtagtgcc
     3121 agcgccgtcc cgaggccagc accgcctcgg cgtcttcgag cccggcgagc agctcggcgc
     3181 gggtgggacc ctccggccgc gaccagatct cgccggcagg ctcgcgctgc agaccggaga
     3241 cgtgaccgag catccgccgc agcgtcaggt cgccgaagcc ggtgccgggc aggtgggcct
     3301 cgagccggtc gtcgaggtcg agcagcccct cgtcgcggca ctgcatcacc agcgtggcgg
     3361 tgaaggtctt ggtgatcgag cccatccgga attgggtgtc cggagtcggc gcggtctcgg
     3421 actcgacgtc ggcgagcccc acctcgtgcg accagaccgg ctcgccgtca cggacgacgg
     3481 cggctgccac ggccgggctg cgccaggcct tctgacggtc ggcggtcatc agggccaggc
     3541 ggcgggccag ctcgtcaggc agcgggcgtg ccgtggcgca ggtgtcggac gtgtcagcgg
     3601 gcgagcgagc cgccatcagc ccgggggcca cgtcacggcg cgaccgccga tgacgtgccc
     3661 gtggacgtgg tcgacggtct gctgggccgc ggcgccgctg ttgaacacga gccggtagcc
     3721 gggcagcccc tcctgcgcgg ccaccgcctg gcactcgcgg accagctcga ccagcgcctc
     3781 ggggtcggcc tcggccaccg cgcccacggt gccgtggtgg tcacgggtga tcacgagcac
     3841 gtgagtgggc gcgatcgggt tgaggtcgcg aaaagcgagc gtgcgctcgc tctcacgcac
     3901 cagggtcgcc gggatctcgc ccgcgacgat cttgcagaac acgcagtcgt cgtcgcgccg
     3961 agtctcggac atgcgcgcga tactacgacg aatcgcgcgt cgggctggtc cggcgccccg
     4021 gtcgacgcgt ccgtgccaac gcatccgtgt caaccggggc gacgagccag gcgtctgacc
     4081 gacgtggaac caacgctgac ctggcgaccg gcccgagggg gttgggtgac gtcctcgacg
     4141 acctgggacg ccgatgaggc cgtcacggcg ctttacaccg cccactaccg ctcgctcgtg
     4201 cgcctgtcgg cgctgctcct gcgcgagcag ggtgccgccg aggaggtcgt gcaggacgcg
     4261 ttcgtggcga tgcacggcgc ctggcgacgg ctgcgcgacc ccgacaagtc gctggcctac
     4321 ctgcgccgga cggtggtcaa ccggtcgcgc tcggcgctgc ggcggcggcg taccgccgag
     4381 aagtacgcgc ccgccccgat gcccgacgcg gccagcgccg agcaccacgc gctcgtggcg
     4441 ctcgagcacc aggcggtggt gcgtgccctg cacacgctgc cgacccggca acgggaaacg
     4501 ctggtgctgc gctactacgc cgacctgtcc gaagcggaga tcgccgaggc gatggggatc
     4561 agccgcggcg cggtcaagag ccacgccagc cgcgggatgg cgaccctccg ctcgaccctg
     4621 gagcagcagt catgacgtac gacgacgacg agctggccgc ccggttgcgc cgggccctgc
     4681 atgcggaggc cgagatggtc aacccacgag gcgatggcct gtcccagatc cgggggcgca
     4741 ccgccggtcg cggttcaccg gggttgatgc gctgggcggt gggcgcggcg gcggccgtgg
     4801 tgatcgccgc ggtcggcgtc ggcgccggcg tgatgtggtc caaccagtcc gacggccagg
     4861 gcgcgatcat cgcgagcacc cccagcgggg ccacctcggc ccccgcgccg acgccatcgg
     4921 agtcgggctc ggccagccca ctgcccggca cccgagtgac ggtgccggtc tactggctcg
     4981 gcgacgccgc cgaccggccg gggctctacc gcgagttcgc ctcggtgctc gccgtcgacg
     5041 cgtccaccct gtcgaaggtc caggtggcgg tgtcgcaagc cctgttcggg acgccggccg
     5101 accctgatta ctcgacgccg tgggccaagg gatcggtcgc gacggtgacg ctgggcgcag
     5161 acgagatcgg catcgacctc aacaaggcgg ccaccgacgg ctcgtcgatg ggctcccaga
     5221 tcgcccagct cgccgtgcag cagctggtct ggaccgccac cgccgccgcg ggcgagaacc
     5281 tgccggtgcg catcaccgtc gagggggcga cccctgacct gttcggcacg gtcagcctgg
     5341 ccgagccgat ccgccgggcc gacccggcct acgaggcgct ggccaacatc tggatcctga
     5401 ccccgaccca gggccaggcg gtgtcgggcg cggtgacggc gaccgggcag gccaccgtcc
     5461 acgaggccaa cctgctgtgg cagctgctca aggggtccac cgtggtcagg aacgacttca
     5521 caacggccag cgagggcggg cccgggcgcg gcgactggac catcgagctg ggcacccttg
     5581 acccgggcac ctacacgctg cgggcctacg ccgagagccc caagggcgac ggcagcctgc
     5641 agggcgagga caccaagacg ttcacggtga agtagccctc agcgccagcg ggtggccgcg
     5701 ctcgcgacag cgagcgcggc tgccccggcg ctggaggtgc gcagcacggt ctcccccagc
     5761 cgtacgacga ccgcgccggc cccgacgaac gcatcgagct cgtcgggcgc gatgccgccc
     5821 tccgggccga cgaccaccac cacgtcgccc gcgtcgggca gcgcgagccc ggccaacggc
     5881 tgctcggccg actcgtgcag cacaagcgca cacgccgcga tctgcagcag cgcgaccacc
     5941 tcagcggtgc tggcgggctc ggtcacctcg gggaaccggg cccggcgggc ctgcttggcc
     6001 gcctcgtggg cggtggcccg ccagcgcgcc ctgcctttgg ccagcttgtc gccgcgccac
     6061 tgcaccaccg agcgggacgc ggcccacggc acgatcacgt cgacccccac ctgggtcatc
     6121 aggtcgacgg ccagctcgcc gtgctcgccc ttggccaggg cctgcacgac aacgaggcgc
     6181 ggagccggtg ccggttcgtc gcgcaccgtg tccacgtcga gctcgacgac gtcgcgcccc
     6241 atgactgcgg ccacccggca cgccgcggcc cggccggtcc cgtcggtgac gacgaccggc
     6301 tcgccggggc gcagccgccg tacggtcgcc gcgtggtggc cctcggggcc ggtcagccgg
     6361 atccgggcac cggccaccgc ctcggccagc gtcgcggcgt cggcgacaaa gaccggcgcg
     6421 ctcactccgg cggcctcagc ggccgttgaa cgcgtcgcgc agccgggaga acagcccctg
     6481 ctggcccggg ctgaactgcc cggccggacg ctcctcgccc cgggcggcgg ccagcttgcg
     6541 cagcagctcc tcctgctcgg cgtcgagctt ggtcggggtg gtcacctcga cgtggacgat
     6601 gaggtcgccg cggccggcgc cgcgcaggtg ggtgacgccc agcccgtgca gcgggatggt
     6661 ctgcccggac tgggtgccgg gccggatgtc gatcgtctcg atgccgtcga gggtctcgag
     6721 ctcgacgctg gtgcccagcg cggccgcggt catcggcagg ctgaccgtgc agtagaggtc
     6781 gtcgccgtgg cgctggaaca gctcatgcgg gcgcaccacc acctcgacgt agaggtctcc
     6841 gggcgccccg ccgccggggc cgacctcacc ctcgccggag agctggatgc gggtgccggt
     6901 gtcgacaccg gccgggatcc ggatggccag agaccggcgg gcccggaccc ggccgtcgcc
     6961 gccgcactcg gcgcacggcg tggagatcac ggtgccgtag ccctggcagg ccacgcacgg
     7021 gcggctggtc atcacctggc cgaggaacga acgggtgacg tgctgcacct cgccgcggcc
     7081 ctggcagacc tcgcaggtgc gcggcgcggt gccgggggcg gcgccgtcgc cgtggcaggc
     7141 ctggcacacg accgcggtgt cgacctgcag ctccttgctg gccccgaaga ccgcctccga
     7201 caggtcgagg tcgagccgga tcagggcgtc ctggccgcgg cgggcccgcg gccgcgggcc
     7261 gcgggcggcg ctggcgccga agaacgcgtc catgatgtcg ctgaagccga acccggcgcc
     7321 gaagccgccg tgaccgccgt cggggtcggc gccgacgtcg tagagctcgc gcttgcgcgg
     7381 gtcggaga
//
LOCUS       DATIBB010000029         3999 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_12205, whole genome shotgun sequence.
ACCESSION   DATIBB010000029 DATIBB010000000
VERSION     DATIBB010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3999)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3999)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3999
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12205"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..511
                     /locus_tag="VLC50_01035"
     CDS             <1..511
                     /locus_tag="VLC50_01035"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017945542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HSF26077.1"
                     /translation="ARPGVRLLVSGHGAADEVLEVASPAVRAATRFLGVTSEADKARM
                     LRTVDVYVAPNLGGESFGIILVEAMAAGTPVVASDLEAFRYVLDEGRLGRLFPTGDTA
                     ALARTLIDLLGDAEARARLSAAGSEAVGRYDWSVVADDVLAVYETVCHGGERVAEDAR
                     PRGWRVRRR"
     gene            524..1063
                     /locus_tag="VLC50_01040"
     CDS             524..1063
                     /locus_tag="VLC50_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013005001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF26078.1"
                     /translation="MGSTTLLTVAVVVVLALAWYLSFSASRLDRLHARVEGARDALDA
                     QLVRRASVALELATSRRLDPASSVLVAEAAHEAREADPDSREVAESTLSRALRAALEQ
                     PGTIEHLAADPVGHDLLVDLHSAARRVELARRFLNDAVRATLAARRDRMVRWFRLAGH
                     AARPDTFEMDDEVPAGLPR"
     gene            1092..2138
                     /locus_tag="VLC50_01045"
     CDS             1092..2138
                     /locus_tag="VLC50_01045"
                     /inference="COORDINATES: protein motif:HMM:NF022702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3048 domain-containing protein"
                     /protein_id="HSF26079.1"
                     /translation="MNGSPHPTARRGRLVALVVGALAVVVACSSPGPTPAVTSPAPGP
                     STAASPTPTPADTSVTSPLTGEKVPALAPVLVVKIGNTPPERPPIGLEAADVVYVEMV
                     EGGLTRYAAVFSSELPERVGPVRSARETDIELLGQYGAVGLAFSGAESSVVSKLRRSS
                     LQLVSFDASIQGFRRDRSRGPAPYNVVGTTAALLKRADNAAVAHDVGFRFGEAPPGGQ
                     RAASMSARYSAARVGATWDAGSGRWLMSMDGRASVAANGARLGAPTVIVQYVTETTLG
                     RRDAAGSRVPFAKTVGAGAAVVLRDGMSYDARWSRSSAGAPTTWTFAGAPMPLAAGPV
                     WVLLVPANRPVQLR"
     gene            2191..3105
                     /gene="pdxS"
                     /locus_tag="VLC50_01050"
     CDS             2191..3105
                     /gene="pdxS"
                     /locus_tag="VLC50_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007535709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HSF26080.1"
                     /translation="MSDPTAPADAPAQTGTARVKRGMAEMLKGGVIMDVVTPEQAKIA
                     EDAGAVAVMALERVPADIRAQGGVSRMSDPDMIDGIIAAVSIPVMAKARIGHFVEAQV
                     LQALGVDYIDESEVLTPADYAHHIDKWKFTVPFVCGATNLGEALRRITEGAAMIRSKG
                     EAGTGDVSNATTHMRDIRAAIARLSSMSTDELYVAAKELQAPYELVKEVAERGALPVV
                     LFTAGGIATPADAAMMMQLGADGVFVGSGIFKSGNPAQRAAAIVKATTFFDDPDVIAK
                     VSRGLGEAMVGINVEELPVDHRLATRGW"
     gene            3235..3756
                     /locus_tag="VLC50_01055"
     CDS             3235..3756
                     /locus_tag="VLC50_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010351155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HSF26081.1"
                     /translation="MSAVPAAPSATDDEVAHELFVAFYGRLAGWVRRLVDDDETAHDI
                     ATESFTRLLAQAGKVHDPRAWLYTTASNLVRDHWRRSARERVALARVGTSADHVRAGR
                     DAYDLDLSTRTTVRALVEALPERLRQPVLLYYYADLSVGQVATALGKAEGTIKRALFD
                     ARALMAAALEDQR"
     gene            3753..>3999
                     /locus_tag="VLC50_01060"
     CDS             3753..>3999
                     /locus_tag="VLC50_01060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF26082.1"
                     /translation="MSPRPGAGGPDLPDELGAIFDQPPAPLPAPAGRFVAIVAAARRR
                     RRRRRFTAAITAAAAVVVLVVGLSAVIRVVTPPAQTVA"
BASE COUNT          485 a         1485 c         1486 g          543 t
ORIGIN      
        1 cgcccggccg ggggtgcggc tcctggtcag cggccacggc gcagcggacg aggtgctcga
       61 ggtggcttcg ccggcggttc gagcggcgac ccgcttcctc ggtgtcacga gcgaggccga
      121 caaggcgcgg atgctccgca ccgtcgatgt gtacgtcgcg ccgaacctgg gcggcgagag
      181 cttcgggatc atcctcgtcg aggcgatggc cgccggcacc ccggtcgtgg ccagcgacct
      241 cgaggcgttc cgctacgtgc tcgacgaggg ccggctcggc cggctgttcc cgaccggtga
      301 caccgcggcg ctggcccgca cgctgatcga cctgctcggc gacgccgagg cgcgggcacg
      361 cctgtcggcg gccgggtcgg aggcggtggg tcgctacgac tggtcggtcg tcgctgacga
      421 cgtgctggcg gtctacgaga cggtctgcca cggcggcgag cgggtcgccg aggacgcccg
      481 cccgcgcggc tggcgggtcc gtcgccgcta gcatcgggct cccatgggct ccacgacctt
      541 gctcaccgtc gccgtcgtcg tcgtgctggc cctggcctgg tacctctcct tctccgcctc
      601 ccgcctcgac cggctgcacg cccgggtcga gggcgcccgc gacgcgctgg acgcccagct
      661 cgtgcggcgg gcctcggtgg cgctcgagct ggcgacctcg cgccggctcg accccgcgag
      721 ctcggtgctg gtggccgagg ctgcccacga ggcgcgcgag gccgacccgg actcccgcga
      781 ggtcgccgag agcaccctgt cgcgggccct gcgggccgcc ctggagcagc cgggcacgat
      841 cgagcacctg gccgccgacc cggtcgggca cgacctgctc gtcgacctgc actcggcggc
      901 ccggcgggtc gagctggccc gccggttcct caacgacgcc gtgcgcgcca cgctggccgc
      961 ccgccgcgac cggatggtcc gctggttccg gctcgccggg cacgctgccc gcccggacac
     1021 cttcgagatg gacgacgagg tgcccgcggg tctgccgcgc tgagttgaca ccggccccta
     1081 cggtgatgcc ggtgaacgga tcgccccatc ccaccgctcg gcgaggccgg ctcgtcgcgc
     1141 tggtcgtcgg cgcacttgcg gtcgtcgtgg cctgctcgtc gccggggccg acgccggccg
     1201 tgaccagccc cgcgcccggg ccgtccacag cggcctcgcc cacacccacc ccggccgaca
     1261 ccagcgtcac ctcgccgctc acgggcgaga aggtgccggc gctggcgccg gtgctcgtgg
     1321 tcaagatcgg caacacgccg ccggagcggc cgccgatcgg cctcgaggcg gccgacgtcg
     1381 tctacgtcga gatggtcgag ggcgggttga cccgttatgc cgcggtgttc tcctccgagc
     1441 tgcccgagcg ggtcggcccg gtgcgcagcg cgcgggagac cgacatcgag ctgctcgggc
     1501 agtacggcgc ggtcgggctg gctttctcgg gcgccgagtc gtcggtggtg agcaagctgc
     1561 gccgctcgtc cttgcagctg gtgtccttcg acgccagcat ccagggcttc cgccgcgacc
     1621 gcagccgcgg cccggcgccc tacaacgtgg tgggcaccac ggcggcgctg ctcaaacggg
     1681 ccgacaacgc cgcggtggcc cacgacgtgg ggttcaggtt cggcgaggca ccgcccggcg
     1741 gccaacgcgc tgcgtcgatg agcgcgcgct actcggccgc ccgggtgggg gcgacctggg
     1801 acgccggcag tggccgctgg ctgatgagca tggacgggcg ggcctcggtg gccgccaacg
     1861 gcgcgcggct gggcgcccca accgtgatcg tccagtacgt caccgagacc acgctgggcc
     1921 gccgcgacgc cgccggctcc cgggtgccct tcgccaagac ggtgggcgcc ggcgcggccg
     1981 tcgtactgcg cgacggcatg tcgtacgacg cccgctggtc gcgctcctcc gcgggggcgc
     2041 cgaccacgtg gacgttcgcc ggggcgccga tgccgctggc ggccgggccg gtgtgggtgc
     2101 tgctggtgcc ggccaaccga cccgtgcagc tgcgttgacc gacgcgccta cgatggaggc
     2161 acaaccgtcc cgtcaccagt gaggtcccca gtgtccgacc cgaccgcccc cgccgacgcg
     2221 cccgcccaga ccggcaccgc ccgcgtcaag cgcggcatgg ccgagatgct caagggcggt
     2281 gtgatcatgg atgtggtcac ccccgagcag gccaagatcg ccgaggacgc cggcgcggtg
     2341 gccgtcatgg cgctggagcg ggtgcccgcc gacatccggg ctcagggtgg cgtctcccgg
     2401 atgagcgacc ccgacatgat cgacggcatc atcgcggcgg tctcgatccc ggtgatggcc
     2461 aaggcccgga tcggccactt cgtcgaggcg caggtgctgc aggcgctcgg ggtcgactac
     2521 atcgacgagt cggaggtgct gacccccgcc gactacgccc accacatcga caagtggaag
     2581 ttcaccgtgc cgttcgtgtg cggggcgacc aacctgggcg aggcgctgcg acgcatcacc
     2641 gagggtgccg cgatgatccg ctccaagggc gaggcgggca ccggtgacgt gtccaacgcc
     2701 accacccaca tgcgcgacat ccgcgcggcg atcgcccggc tgtcctcgat gagcaccgac
     2761 gagctctacg tcgcggccaa ggagctgcag gcgccctacg agctggtcaa ggaggtcgcc
     2821 gagcggggcg cgctgcccgt cgtgctgttc actgccggcg gcatcgccac gccggccgac
     2881 gcggcgatga tgatgcagct gggcgccgac ggcgtgtttg tcggttcggg catcttcaag
     2941 tcgggcaacc cggcgcagcg ggcggccgcg atcgtcaagg ccaccacgtt cttcgacgac
     3001 cccgacgtga tcgccaaggt ctcgcgcggg ctgggtgagg cgatggtggg catcaacgtc
     3061 gaggagctgc ccgtcgacca ccggctggcc acccgcggct ggtagtcccc ggcccggccc
     3121 gtgggccggc aaccggatcg ggcgctgcgg cgtcttcgag atgtgagcct gactgtcgcg
     3181 acccccgtcg cgaaggacct gggctcctcg acccggtggc gcgaaggggg cagcgtgtca
     3241 gctgtgccgg ctgcgcccag tgccaccgac gacgaggtgg cccacgagct gttcgtggcg
     3301 ttctacggcc ggctggccgg ttgggtccgc cggctcgtcg acgacgacga gaccgcgcac
     3361 gacatcgcca ccgagtcgtt cacccggctg ctggcgcagg cgggcaaggt ccacgacccc
     3421 cgcgcctggc tctacacgac cgccagcaac ctcgtgcgcg accactggcg tcggtccgcg
     3481 cgcgagcgcg tggccctggc ccgggtgggg acctccgccg accacgtccg ggccggtcgc
     3541 gacgcctatg acctcgacct gagcacccgc accacggtgc gcgccctggt cgaggcgctg
     3601 cccgagcggc tgcgccagcc ggtgctgctc tactactacg ccgacctgtc ggtgggccag
     3661 gtggccaccg cgctgggcaa ggccgagggc acgatcaagc gagcactctt cgacgctcgg
     3721 gccttgatgg ccgcggctct ggaggatcag cgatgagccc gcgacccgga gccggagggc
     3781 ccgacctgcc cgacgagctc ggcgcgatct tcgaccagcc gccggcgccg ctgccggcac
     3841 cggccgggcg gttcgtggcg atcgtggccg ctgcccggcg tcggcggcgc cgccggcggt
     3901 tcacggcggc gatcacggcg gccgccgccg tggtggtgct cgtggtgggt ctctcggcgg
     3961 tgatccgcgt cgtgacgccg cctgcgcaga ccgtggccc
//
LOCUS       DATIBB010000030         3236 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_1064309, whole genome shotgun sequence.
ACCESSION   DATIBB010000030 DATIBB010000000
VERSION     DATIBB010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3236
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1064309"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1955)
                     /locus_tag="VLC50_01065"
     CDS             complement(<1..1955)
                     /locus_tag="VLC50_01065"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HSF26083.1"
                     /translation="MRSSTRARLGLVAAAATALAVAITPSGATAGVGSDGSNPSSDGP
                     RAAHGVDSSSAIVRLSADPLATSPRTRPAPGTKINFSSSTVKSERALLAAARNDFKSW
                     LRANAPAARVVGEYDVALNAVSVRLNGTSLDTLRSAPGVVSASYEALYTPLRGGRSTP
                     RVPSGTSITSTSQDPDLGLIAAVPANDRVGWGVQVAIIDTGIDVTHPCFSGDGFPSTK
                     QLGDTNLTNKKVIVAKVFNNKATSRGYTAFAEQDHGTHVAGTVACDLGTHAVVSGTVI
                     PYDVSGVAPGAQLGNYNVFPGDVLDARSEDILDALQAAYLDGMDVANLSLGGGANGNQ
                     DLLTVAIDNLDRAGMVVAVAAGNSGPGPFTVESPGSAARALTAGASTVGHFVGAPVTV
                     GGTSYGAASGDFATVSADLTADLGVVADADGGLGQACSALESGSLAGKIALVARGTCT
                     FSTKVKVAEDAGAAAVLVANNVAGDPVAMSADATVQPAPEIPAYMVAQETGAKLLSSS
                     GATATIGATLSYLPTGNDNIMAGFSSQGPTDVDYRVKPDVVAPGVNVLSSIPLHFCRA
                     GEGTCWAFFQGTSMATPHLAGMAAALLSKHKGWSAEQVRSAVVNTAVDDLLTDFQTGE
                     SVVKDVQITGAGLANLAAASDDQVAVGP"
     gene            complement(2130..2483)
                     /locus_tag="VLC50_01070"
     CDS             complement(2130..2483)
                     /locus_tag="VLC50_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018347930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin"
                     /protein_id="HSF26084.1"
                     /translation="MPELIDSPTRIPVPGGKVILEYVGRVATASTEVSVAHMQAPAGW
                     DEPAQTPEFDEVTLVLAGSLLVEHSHGTLAVDAGHAVLTHAGERVRYVAGPDGADYVA
                     VCLPAFAPELAHREE"
     gene            complement(2548..>3236)
                     /locus_tag="VLC50_01075"
     CDS             complement(2548..>3236)
                     /locus_tag="VLC50_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HSF26085.1"
                     /translation="LDVKVGSGAFMKTLDQARELARTMVDLGTDAGVRTVALLTNMAT
                     PLGLTVGNALEVRESLEVLAGGGPGDVVELTLALAREMLAAAEVDGKDPADALRDGSA
                     MDVWRAMVSAQGGDPDAALPTARETHALLAPADGVLTRLDAYQVGLAAWRLGAGRARK
                     EDPVQAAAGIELHAKPGIEVRGGQPLLTLHTDEPDRFERALAALEGAFEIAPEGSSPD
                     LPDTVIERVS"
BASE COUNT          461 a         1132 c         1187 g          456 t
ORIGIN      
        1 gggccgacgg cgacctggtc atccgaagcc gcggccaggt tggccaggcc cgcaccggtg
       61 atctggacgt ccttcacgac agattccccg gtctggaagt cggtcagcag gtcgtccacg
      121 gcggtgttga ccaccgccga gcggacctgc tcggcgctcc aacccttgtg cttactgagc
      181 agggcggctg ccattccggc caggtgtggc gtcgccatcg acgtgccctg gaagaacgcc
      241 cagcaggtgc cctcgccggc cctgcagaag tgcagcggga tggacgagag cacgttgacg
      301 ccgggggcca ccacgtcggg cttgactcgg taatcgacgt cggtcgggcc ctggctggag
      361 aagccggcca tgatgttgtc gttgccggtc ggcaggtagc tcagcgtcgc gccgatggtc
      421 gcggtcgcgc ccgacgagga aagcagcttg gcgccggtct cctgggccac catgtaggcg
      481 ggaatctccg gcgcgggctg cacggtggca tcggccgaca tcgcgacggg gtcgccggcc
      541 acgttgttgg ccacgagcac cgccgccgca ccggcatcct cggccacctt gaccttggtg
      601 gagaaggtgc acgtgccacg agccacgagc gcgatcttgc cggcgaggct gcccgactcc
      661 agcgcggagc aggcttgccc gaggccgccg tcggcgtctg cgacgacccc caggtccgcg
      721 gtgaggtcgg ccgacacggt ggcgaagtca ccgctggccg ccccgtagga cgtgccgcca
      781 acggtcaccg gggctccgac gaagtgaccg acggtcgagg cgccggcggt gagtgcgcgc
      841 gccgcagaac ccggcgactc caccgtgaag ggaccagggc cggagttgcc cgccgccacc
      901 gcgaccacca tgccggcccg gtcgaggttg tcgatggcca cggtgagcag gtcctggttg
      961 ccgttcgccc caccgccgag gctcaggttg gccacgtcca tgccgtcgag gtaggccgcc
     1021 tgcagcgcgt cgaggatgtc ctcggagcgg gcatcgagca cgtcgccggg gaagacgttg
     1081 tagttgccga gctgcgcccc gggggccacc ccggacacgt cgtaggggat cacggtgccg
     1141 ctgacgaccg cgtgtgtgcc gagatcgcag gcgaccgtgc ccgcgacatg cgtgccgtgg
     1201 tcctgctcgg caaaggcggt gtagccccgc gaagtggcct tgttgttgaa gaccttcgcc
     1261 acgatgacct tcttgttggt caggttcgtg tcacccagct gtttggtgga ggggaaccca
     1321 tcccccgaga agcacgggtg ggtcacgtcg atgccggtgt cgatgatcgc gacctggacg
     1381 ccccacccca cccggtcgtt cgccggcacc gcggcgatga ggccgaggtc tgggtcctgt
     1441 gacgtcgagg tgatcgacgt gccagacggc acccgaggcg tagacctgcc gccgcggagc
     1501 ggcgtgtaga gcgcctcgta ggacgccgaa acgacacccg gcgccgaccg cagggtgtcg
     1561 agcgaggtgc cgttgagccg caccgacacc gcgttgagcg cgacgtcgta ctcccccacg
     1621 accctggcgg cgggcgcgtt ggcccgcagc cagctcttga agtcgttgcg ggcggcggcc
     1681 agcagggccc gctctgactt caccgtcgag gacgagaagt tgatcttcgt gccgggtgcc
     1741 ggccgggtgc gcggcgacgt ggccagcgga tcggcactga gtctgacgat ggccgacgac
     1801 gagtcgaccc catgggcggc gcgcgggccg tcgctgctcg ggttggagcc gtcggacccg
     1861 acgccggcgg tggcaccgga cggggttatc gcaacggcta gggcggtggc agccgcggcg
     1921 acgaggccga ggcgcgctcg agtggaactt ctcacgtgga cccccagatc acgtgtggtg
     1981 ggaggcgcac gcggctggac gcctcctggc accacgcaac gtaatcccgt caggggacga
     2041 cagtgaggca aaagaacaga catctccccc atgtggggga cgtcatgact agagcggcgc
     2101 gtcggggacg ccagggcgct gtctgccggt cactcctcgc ggtgggccag ctcgggcgcg
     2161 aacgccggca ggcagaccgc gacgtagtcg gcgccgtcag ggccggcgac gtagcgcacg
     2221 cgttcgccgg cgtgggtcag caccgcgtgc ccggcgtcaa ccgcgagggt gccgtggctg
     2281 tgctccacga gcaggctccc cgccagcacc agcgtgacct cgtcgaactc gggggtctgc
     2341 gccggctcgt cccagcccgc cggggcctgc atgtgggcca ccgacacctc ggtggaggcg
     2401 gtggccacgc ggccgacgta ttcgaggatg accttgccac ccggcaccgg gatgcgggtg
     2461 ggcgagtcga tcagctccgg catgatcggc agcgtacgac ggccagcggc ctcggcgtcg
     2521 ctgcatcacc cactcgaacc cggcccatca gctgacccgc tcgatcaccg tgtcgggaag
     2581 gtcggggctg gagccctcgg gcgcgatctc gaaggcgcct tccagcgcgg cgagcgcccg
     2641 ctcgaaccgg tcgggctcgt cggtgtgcag cgtcagcagc ggctgcccac cgcgcacctc
     2701 aatgcccggc ttggcgtgca gctcgatccc cgcggcggcc tgcaccgggt cctccttgcg
     2761 ggcccgtccg gcgcccagcc gccacgcggc cagcccgacc tggtaggcgt cgagccgggt
     2821 cagcaccccg tcggccgggg ccagcagggc gtgggtctcg cgggcggtgg gcagcgcggc
     2881 gtcgggatca ccgccctggg cgctgaccat cgcccgccac acgtccatcg ccgagccgtc
     2941 gcgcagcgcg tcggccgggt ccttgccgtc gacttcagcg gcggccagca tctcgcgggc
     3001 gagcgccagg gtcagctcga cgacgtcgcc gggcccaccc ccggcgagca cctcgagcga
     3061 ctcgcgcacc tcgagcgcgt tgccgaccgt gagcccgagc ggggtggcca tgttggtgag
     3121 cagcgccacg gtgcgcacac cggcgtcggt gcccaggtcg accatggtcc gggccagctc
     3181 acgggcctgg tcgagcgtct tcatgaacgc ccccgacccg accttgacgt cgagca
//
LOCUS       DATIBB010000031         2644 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_538712, whole genome shotgun sequence.
ACCESSION   DATIBB010000031 DATIBB010000000
VERSION     DATIBB010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2644
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_538712"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(11..1069)
                     /gene="thrC"
                     /locus_tag="VLC50_01080"
     CDS             complement(11..1069)
                     /gene="thrC"
                     /locus_tag="VLC50_01080"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016438254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HSF26086.1"
                     /translation="MARPWRGVIEEYRDRLPVSAATPVVTMAEGGTPLVRARVLSERT
                     GAQVWLKFEGANPTGSFKDRGMTLAISKAAEAGAHAVICASTGNTSASAAAYAVRAGM
                     VAAVLVPEGNIAMGKLSQALAHGATILQVDGNFDDCLVLARDLSEHYPVALVNSVNPF
                     RIEGQKTAAFEIVDELGDAPDIHCLPVGNAGNITAYWKGYREYADDGPATRWPRMWGF
                     QAAGAAPIVRGEPVTSPQTVATAIRIGNPASWKQALAARDESDGLIDAVSDEAILAAH
                     RLLSAEEGVFVEPASAASVAGLLQCHERGLLQPGLCVVCTVTGHGLKDPQWAIKAAPA
                     PIGIPADVARAAAELGLA"
     gene            complement(1072..2415)
                     /locus_tag="VLC50_01085"
     CDS             complement(1072..2415)
                     /locus_tag="VLC50_01085"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010468706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HSF26087.1"
                     /translation="MAERPLRVALLGCGVVGAEVARLLIAQHDDLAARVGRPLELAGV
                     AVRRPARHRDVPGLDPTLLTDDAAGLVRRDDVDLVVEVVGGIEPARSWLLAAMDHGKS
                     VVSANKALLAEDGATLVAAAEKNGVDLFYEAAVAGAIPIVRPLRESLVGDRISTVLGI
                     VNGTTNFILTRMDETGAGFAEALDEATALGYAEADPTADVEGFDAAAKAAILASLAFH
                     TRVTASDVHREGITEVTAGDVASAKRMGCVVKLLAICERSDDGRGVSVRVHPAMIPRS
                     HPLAGVREAFNAVYVEAEAAGELMFYGRGAGGAPTASAVLGDLVAVARHRTNGGHGAR
                     ESSYADLEIRPMGLTVTRYHVSLDVADRPGVLAAVAAVFARHGVSIETVRQQQHVTGA
                     GVVGDGGLQAPAGPDAELVVVTHAAPDEALGATVDSLRDLDIVRAVTSVMRVEGD"
BASE COUNT          336 a          985 c          952 g          371 t
ORIGIN      
        1 tcccccatgg tcaggcgagc ccgagctcgg cggcggcgcg ggcgacgtcg gccgggatgc
       61 cgatcggggc cggtgccgcc ttgatggccc actgcgggtc cttgagcccg tgcccggtca
      121 cggtgcacac gacgcacagg ccaggctgca ggagaccgcg ctcgtggcac tgcagcagtc
      181 cggcgacgct ggcggccgag gccggctcga cgaagacgcc ctcctcggcg ctgagcagcc
      241 ggtgcgcggc caggatcgcc tcgtcgctga ccgcgtcgat gagcccgtcg gactcgtcgc
      301 gggccgcgag cgcctgcttc caggaggccg ggttgccgat tcggatcgcg gtcgccaccg
      361 tctgcggcga ggtcaccggc tccccacgca cgatcggggc cgccccggcg gcctggaagc
      421 cccacatccg cggccaccgg gtggccgggc cgtcgtcggc gtactcgcga tagcccttcc
      481 agtaggccgt gatgttgccc gcgttgccga ccggcaggca gtggatgtcg ggcgcgtcgc
      541 cgagctcgtc gacgatctcg aacgcggcgg tcttctgccc ctcgatccgg aacgggttga
      601 ccgagttgac cagggccacc gggtagtgct cggacaggtc gcgggcgagc accaggcagt
      661 cgtcgaagtt gccgtcgacc tgcaggatcg tcgcgccgtg cgccagcgcc tggctgagct
      721 tgcccatcgc gatgttgccc tcgggcacca gcaccgccgc gaccatcccg gcacgtacgg
      781 cgtaggcggc cgcggacgcg ctcgtgttgc cggtcgaggc gcagatcacc gcgtgggcgc
      841 cggcctcggc cgccttggag atggccaggg tcatgccccg gtccttgaac gacccggtcg
      901 ggttggcgcc ctcgaacttc agccacacct gcgcgccggt gcgctcggag agcacccggg
      961 cccgcacgag cggggtgcca ccctcggcca tcgtcaccac gggcgtcgcg gcgctgaccg
     1021 gcaaccggtc gcggtactcc tcgatcacgc cccgccaggg tcgggccacg gtcagtctcc
     1081 ttctacgcgc atcacgctgg tcaccgcacg gacgatgtcg aggtcgcgca gcgagtcgac
     1141 ggtcgcgccc agcgcctcgt cgggggcggc gtgggtcacc accacgagct cggcgtccgg
     1201 gccggccggt gcctgcaggc cgccgtcacc gaccaccccg gccccggtca cgtgctgctg
     1261 ctggcgcacc gtctcgatcg agaccccgtg ccgggcgaag accgcggcca ccgcggccag
     1321 cacgccgggc cggtcggcca cgtcgaggct gacgtggtag cgggtgacgg tcaggcccat
     1381 cggccggatc tcgagatcgg cgtacgacga ctcgcgggca ccatgcccac cgttggtccg
     1441 gtggcgggcc acggcgacca ggtcgccgag caccgcgctc gcggtcggcg cgccgccggc
     1501 gccgcggcca tagaacatca gctcgccggc ggcctcggcc tcgacgtaga ccgcgttgaa
     1561 cgcctcgcgg accccggcca gcgggtggct gcgagggatc atcgccgggt gcacccgcac
     1621 gctgaccccg cgtccgtcgt cggagcgctc gcagatggcc aacagcttga ccacgcagcc
     1681 catccgcttg gcgctggcca cgtcgccggc ggtcacctcg gtgatgcctt cacggtgcac
     1741 gtcgctggca gtgacccggg tgtggaaggc caggctggcc aggatcgccg ccttggccgc
     1801 ggcgtcgaag ccctcgacgt cggcggtcgg gtcggcctcg gcgtagccga gcgcggtggc
     1861 ctcgtcgagc gcctcggcga accccgcacc ggtctcgtcc atccgggtga ggatgaagtt
     1921 ggtggtgccg ttgacgatgc cgagcaccgt gctgatccgg tcaccgacca gcgactcgcg
     1981 cagcgggcgc acgatcggga tcgccccggc caccgcggcc tcgtagaaga ggtccacgcc
     2041 gttcttctcg gcggcggcga ccagcgtcgc gccgtcttcg gcgagcagcg ccttgttggc
     2101 gctgaccacg ctcttgccgt ggtccatcgc ggccagcagc cagctgcggg ccggttcgat
     2161 gccgccgacc acctcgacca cgaggtcgac gtcgtcgcgg cggaccagcc cggcggcgtc
     2221 atcggtcagc agggtgggat caaggcccgg tacgtcgcgg tggcgcgccg ggcgtcgtac
     2281 ggcgacaccg gccagctcga gcggtcggcc cacccgggcg gccaggtcgt cgtgctgcgc
     2341 gatcagcagg cgggcgacct cggcgccgac gacgccgcag ccgagcaggg ccacgcgcag
     2401 tggtcgttca gccatgtcgt cccttcctcg gtcgccacgt ttgtcgcaca tcaacggccc
     2461 tcggcgctct ccgtcccggc atctgagacg tgctcgccac gctgatcact cgacgtccag
     2521 gcgcagcagg tcgtccaccg tctcgcggcg gacgatcacc cgtgcggtgc cattgcgcac
     2581 cgcgaccacc ggcggacgcg gcacgtggtt gtagctgctg gccatgctcc ggcagtaggc
     2641 gccg
//
LOCUS       DATIBB010000032         8127 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_1197960, whole genome shotgun sequence.
ACCESSION   DATIBB010000032 DATIBB010000000
VERSION     DATIBB010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 8127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8127
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1197960"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            168..725
                     /locus_tag="VLC50_01090"
     CDS             168..725
                     /locus_tag="VLC50_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial proteasome activator family protein"
                     /protein_id="HSF26088.1"
                     /translation="MTQQPQQPEAQDSQEQQRVVVVTQDGMGVSPHPADDDDTTNPAD
                     LVEQPAKVMRIGSMIKQLLEEVRNAPLDEAGRVRLAEIHRRSIEELKDGLAPELTDEL
                     ERIALPFGEQAPTDAELRIAQAQLVGWLEGLFHGIQTALFAQQMAARAQFEEMRRRAL
                     PAGMMPGMAPPDQAGDHPGSTGQYL"
     gene            complement(789..1607)
                     /locus_tag="VLC50_01095"
     CDS             complement(789..1607)
                     /locus_tag="VLC50_01095"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018092166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HSF26089.1"
                     /translation="MAESVTPRLVATDLDGTILRTDGTVSARTVAALRDAEARGLTVV
                     LVTGRPPRWMGPVVEALGHTGLAVCANGALVLDLHHGDPGTVVAEQTIEVAVLTEVVA
                     ALRRVEPTLAFGVEYADGFVHESTYQPGATAVGPTVPRPVDLDALLSRPAVKLLAKHP
                     DGDPDVLLQMAREVLGDVVEMTHSSRDGALLEISAVGVSKASALAALCAERGLGADEV
                     VAFGDMPNDLPMLAWAGTSYAMANAHRDVLAAVPRHAPSNDDDGVAVILERFLR"
     gene            complement(1611..2462)
                     /locus_tag="VLC50_01100"
     CDS             complement(1611..2462)
                     /locus_tag="VLC50_01100"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007924874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HSF26090.1"
                     /translation="MSLAAAAPAPGSDGWVPELVALDVDGTLSDYDGRISEPVRRMVS
                     RVRERGAHVVLATGRSVLATRPIAETLGLDAGLMVCSNGAVLAGITPPVVLEATTFDP
                     SAAVRALLELAPDTLVAVEDVGRGYLVTEPFPDGELMGEQTVCPLAEMMGRPVTKVVL
                     RRPDSTPEAFFTLVEQAGLHGVNYAIGYSAWLDVLPRNVSKASTLERVRRQLGVASSA
                     TLAVGDGRNDLEMLAWAGWSVAMGNASPEVQDAADEVAWPVTEDGLVRALARWFPPLD
                     GADGVGG"
     gene            complement(2494..3771)
                     /gene="serS"
                     /locus_tag="VLC50_01105"
     CDS             complement(2494..3771)
                     /gene="serS"
                     /locus_tag="VLC50_01105"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017573232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HSF26091.1"
                     /translation="MIDPRILRDEPDRVRASQRARRADEQLVDAAIAADQDRRAALVA
                     FETLRAEQKALGKQVAAAKGEEKSRLLERTKALAQQVKEADAAAGAAGDRYEAAIRAL
                     PNLVQDGAPEGGEDDYVVLETVGTPRDFAKEGFTPRDHLELGERLGAIDMDRGAKVSG
                     ARFYFLTGVGAQLQLALLSLGIQQATAAGFIPMITPSLVRPEAMDGTGFLGQAAADVY
                     RLEADDLYLVGTSEVALAAYHGDEILEAASLPRRYAGWSSCFRREAGSHGKDTRGIIR
                     VHQFDKVEMFSYVHPDEAAAEHLRLLAWEKEMLAKVEVPYRVIDVAAGDLGLSAARKY
                     DCEGWVPSQGRYRELTSTSNCTTFQSRRLSVRYRRDDRSTDYVATLNGTLATTRWLVA
                     ILENHQQPDGSVRVPAALRPWLGGREVLEPVDG"
     gene            complement(3939..5213)
                     /locus_tag="VLC50_01110"
     CDS             complement(3939..5213)
                     /locus_tag="VLC50_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011362722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4143 domain-containing protein"
                     /protein_id="HSF26092.1"
                     /translation="MDHYLPRTVDDELDELMSELSAVVLEGPKGVGKTVTASRRAQRV
                     MALDDPATLEVVVADPSQLDAVPGTVLLDEWQRHPPVWDFIRRRVDAGAPPGRFLLTG
                     SAVPAHAAVHSGAGRIVSVRMRPLSLPERGLAEPTVSLARLLDGHRAAIEGSCDVGLD
                     DYVEQILASGLPAIRTLSGRARRAQLAGYLSRVVEHEFPEQGLRVRRPETLRGWLTAY
                     AAATATTASYNAILDAATPGRSDKPAKTTTIAYRDVLAQLWLLDPVPAWLPSHNRLHR
                     LTQAPKHHLADPALAAHLLGVDAGALLSGERSGPLVPRDGTLLGALFESLVVLSVRVA
                     AQAAQARVHHLRTQNGRHEVDVIVERPDGRVVALEVKLAGTVDDASVAHLHWLRTQVG
                     EDLIDAAVITTGRHAYRRPDGIAVVPAALLGA"
     gene            complement(5312..6244)
                     /gene="pheA"
                     /locus_tag="VLC50_01115"
     CDS             complement(5312..6244)
                     /gene="pheA"
                     /locus_tag="VLC50_01115"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019633605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HSF26093.1"
                     /translation="MIVARFGYLGPEGTFTESALRTLPAAAEAELIAYPTVPSALDGV
                     RRGEVAAAMVPIENSVEGAVTATLDELATGDPLMVTREVHLPVAFALLARPGTALSAV
                     RGVGTHPHALAQCRRWLARTLPDAALIAATSTAAAAAAVAAGESSYDAALTAPVAVEH
                     YPLEVLADNIADNRRAVTRFVLVGRPAAPPAPTGTDKTTVVAYIRDDRPGALLEILTE
                     FAVRGVNLTRIESRPTGDRLGDYHFSIDCEGHVADARVGEALMGLRRVCADVRFLGSY
                     PRADKVAPTVRPGTSGAEFADAQAWLAGIRAGNA"
     gene            complement(6324..6833)
                     /locus_tag="VLC50_01120"
     CDS             complement(6324..6833)
                     /locus_tag="VLC50_01120"
                     /inference="COORDINATES: protein motif:HMM:NF025935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4446 family protein"
                     /protein_id="HSF26094.1"
                     /translation="MPLDPQTLDLLAIGGVALGLLGLTFALVANRRIGRLRRSYDLLQ
                     GDGRSDDFVTAVERQIDAVEALRAEVHAARRENADTRQALEEAIRHVAVVRYDAFSDM
                     GGRMSFSVALLDDDGDGLVLTTITGRRDTRTYAKGLRGGRSDQALSPEEEQAIAFALE
                     SAPGHAAVR"
     gene            6967..7824
                     /gene="larB"
                     /locus_tag="VLC50_01125"
     CDS             6967..7824
                     /gene="larB"
                     /locus_tag="VLC50_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006542656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HSF26095.1"
                     /translation="MDREDVAALLDAVATGQVSTEAALARLAAGPLDGGPLGDGFADL
                     GYARLDTHRALRTGDPEVVYGAGKTPAQTVELLRALRSAHPNRPALATRLPAPAQGAV
                     RAAFPDAQIDPSARTAVVGPLPSPRGTVVVVAAGTSDAAVAAEAATTARAFGAGVTRL
                     DDVGVAGIHRLLAARATLDTADCLIVVAGMEGALPSVVGGLTGVPLVAVPTSVGYGAS
                     FGGLAALLGMLNACAPGVVVANIDNGFGAGVFAARVARRAAPRTSAPATDPASTDAIA
                     PATNEAGAT"
     gene            7821..>8127
                     /gene="larC"
                     /locus_tag="VLC50_01130"
     CDS             7821..>8127
                     /gene="larC"
                     /locus_tag="VLC50_01130"
                     /EC_number="4.99.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF014072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel insertion protein"
                     /protein_id="HSF26096.1"
                     /translation="MTRIGWIDATVGAAGDMLAAALVGAGASYDVMAEAVAAVGVPVT
                     TAITTVDAQGLAAWRWSVRTDETDPPRRTWADIRARLDAADLDPAVRALAHDAFERLA
                     "
BASE COUNT         1101 a         3000 c         2991 g         1035 t
ORIGIN      
        1 accgttgctg ccaccgcccg ccgtctcggc cgcgaggccg cggacgcggt ctggaccatg
       61 ggggcccagt cgtatgccgg gtacgccaac taccggcagt gggcggcagg tcgggacatc
      121 accgtctggc tgctcagccc gcgcgacgcg tgaggcagga tggacccatg acccagcagc
      181 cgcagcagcc cgaggcacag gactcccagg agcagcagcg cgtcgtcgtt gtgacccagg
      241 acgggatggg ggtgtcgccc caccctgcgg acgacgacga cacgaccaac ccggctgacc
      301 tcgtcgagca gccggccaag gtcatgcgca tcgggtcgat gatcaagcag ctgctcgagg
      361 aggtccgcaa cgcgccgctc gacgaggcgg gtcgcgtgcg cctggccgag atccaccggc
      421 gctccatcga ggagctcaag gacggcctcg cccccgagct caccgacgag ctcgagcgca
      481 tcgcacttcc cttcggcgag caggccccga cggacgccga gctccgcatc gcgcaggcgc
      541 agctcgtcgg ctggctcgag ggcctcttcc acggcatcca gaccgcgctg ttcgcccagc
      601 agatggccgc gcgggcccag ttcgaggaga tgcgccggcg ggcgctgccg gccgggatga
      661 tgcccgggat ggccccgccg gaccaggcgg gcgaccaccc ggggtcgacc ggccagtacc
      721 tctgacggcg tcgccccggg acccgctcgc gccgaccgcg gacccgctcg ccacgaccgc
      781 gggcgggatc agcggaggaa gcgctcgagg atgaccgcca cgccgtcgtc gtcgttgctc
      841 ggcgcgtgcc ggggcaccgc ggccagcacg tcgcggtggg cgttggccat cgcataggac
      901 gtgccggccc aggccagcat cggcaggtcg ttgggcatgt cgccgaacgc caccacctcg
      961 tcggcgccca gcccgcgctc ggcgcacagc gccgccagcg cgctggcctt gctgacgccg
     1021 acggcgctga tctccagcag cgccccgtcg cgcgaggagt gcgtcatctc caccacgtcg
     1081 ccgagcacct cacgggccat ctggagcagc acgtcggggt caccgtcagg gtgcttggcc
     1141 aacagcttga ccgccggccg ggacagcagc gcgtcgagat cgaccgggcg cggcaccgtc
     1201 ggcccgacgg cggtggcgcc cggctggtag gtcgactcgt gcacgaaccc gtcggcgtac
     1261 tccacgccaa acgccagcgt cggctcgacc cggcgcagcg cggcgaccac ctcggtgagc
     1321 accgccacct cgatggtctg ctcggccacc acggtgcccg ggtcgccgtg gtgcaggtcg
     1381 agcaccagcg ccccgttggc gcacactgcc agcccggtgt gcccgagcgc ctcgacgacc
     1441 gggcccatcc agcgcggcgg gcggccggtc accagtacga cggtcagccc gcgcgcctcg
     1501 gcgtcgcgca gcgcggccac cgtgcgggcc gagaccgtcc cgtcggtgcg caggatcgtg
     1561 ccgtcgaggt cggtggcgac cagccgcggg gtgaccgact cagccatcgc tcagccgccc
     1621 acgccgtccg cgccgtcgag gggcgggaac cagcgggcca gcgcgcggac cagcccgtcc
     1681 tcggtgaccg gccaggccac ctcgtcggcg gcgtcttgca cctcgggcga ggcgttgccc
     1741 atcgccaccg accagccggc ccaggccagc atctcgaggt cgttgcgccc gtcgccgacg
     1801 gccagcgtcg ccgacgacgc gacgcccagc tggcgccgta cccgctcgag ggtcgaggcc
     1861 ttggacacgt tgcggggcaa cacatccagc caggcggagt agccgatcgc gtagttgacc
     1921 ccgtgcagcc cggcctgctc gaccagcgta aagaacgcct ccggggtcga gtcgggccgg
     1981 cgcagcacca ccttggtcac cggccggccc atcatctcgg ccagcgggca gacggtctgc
     2041 tcgcccatca gctcgccgtc ggggaacggc tcggtgacga ggtagccgcg cccgacgtcc
     2101 tcgaccgcga ccagggtgtc gggggccagc tcgagcagcg cgcgtacggc ggccgacggg
     2161 tcgaacgtcg tcgcctcgag caccaccggc ggggtgatcc cggcgagtac ggcgccgttg
     2221 ctgcacacca tcagcccggc gtccaggccg agcgtctcgg cgatcgggcg ggtggccagc
     2281 accgaccggc cggtggccag caccacgtgc gcgccgcgct cacggacccg gctcaccatc
     2341 cggcgcaccg gctcggagat ccgcccgtcg tagtcggaca gcgtgccgtc cacgtcgagc
     2401 gcgaccagct cgggcaccca gccgtcggag ccgggcgcgg gtgctgccgc ggccaggctc
     2461 accggcgctc cgtcgtacgg cgcatgcggg gcctcagccg tccaccggct cgagcacctc
     2521 acgcccgccc agccacggcc gcagcgccgc cggcacccgc accgagccgt cgggctgctg
     2581 gtggttctcg aggatggcga ccagccagcg ggtggtggcc agggtgccgt tgagcgtggc
     2641 gacgtagtcg gtggaccggt cgtcgcggcg gtagcgcacc gacagccgac gggactggaa
     2701 cgtcgtgcag ttggaggtcg acgtgagctc gcggtagcgg ccctggctgg gcacccagcc
     2761 ctcgcagtca tacttgcgcg ccgccgagag gcccaggtcg ccggccgcca cgtcgatcac
     2821 ccggtagggc acctcgacct tggccagcat ctccttctcc cacgcgagca gccgcaggtg
     2881 ctcggcggcc gcctcgtcgg ggtggacgta gctgaacatc tccaccttgt cgaactggtg
     2941 cacccggatg atgccgcggg tgtccttgcc gtgcgagccg gcctcccggc ggaagcacga
     3001 ggaccagccg gcgtagcggc gcggcaggct ggccgcctcg aggatctcgt cgccgtggta
     3061 ggcggccagc gcgacctcgg aggtgccgac caggtagagg tcgtcggcct ccagccggta
     3121 gacgtcggcg gcggcctggc cgaggaaccc ggtgccgtcc atcgcctccg gccggaccag
     3181 cgacggggtg atcatgggga tgaacccggc ggcggtggcc tgctggatgc ccagggacag
     3241 cagggcgagc tgcagctggg cgccgacgcc ggtgaggaag tagaaccggg ccccggacac
     3301 cttggcgccg cggtccatgt cgatcgcgcc gagccgctcg cccagctcga ggtggtcgcg
     3361 cggggtgaac ccctccttgg cgaagtccct tggggtgccg acggtctcga gcacgacgta
     3421 gtcgtcctcg ccgccctccg gggcgccgtc ctgcacgagg ttgggcagcg cgcggatcgc
     3481 ggcctcgtag cgatcgccgg cggcgcccgc ggcggcatcg gcctccttga cctgctgggc
     3541 cagcgccttg gtgcgctcga gcagcctcga cttctcctcg cccttggcgg cggcgacctg
     3601 cttgcccagc gccttctgct cggcgcgcag cgtctcgaac gcgacgagcg cggcgcggcg
     3661 gtcttggtcg gcggcgatcg cggcgtcgac gagctgttcg tcggcgcggc gggcacgctg
     3721 ggaggcgcgg acccggtcgg gttcgtcacg aaggatgcga gggtcgatca cgcgtcgagg
     3781 ttagtcgagg gcagaccggc gccgtcgggc cgccgtacgc cgcacgcccg agggacggcg
     3841 gacactaggg acggaacaac gcagccgtac gacgtctgct cgtgctcact gacactgcgg
     3901 cgacccgatt gttccgtcct taacgtccgc cgcggacgtc aggcccccag cagcgcagca
     3961 ggcaccaccg cgatcccgtc cgggcgtcga taggcgtgcc ggccggtcgt gatcaccgcg
     4021 gcatcgatca ggtcttcgcc cacctgggtc cgcagccagt gcaggtgcgc aacgctggcg
     4081 tcgtcgacgg tgccggccag cttcacctct agagcgacga cgcgaccatc gggccgctcg
     4141 acaatgacat cgacctcgtg ccggccgttc tgggtgcgca ggtggtgcac ccgtgcctgc
     4201 gccgcctgcg cagccacgcg cacgctgagc accaccaacg attcgaacaa ggcgccgagc
     4261 agcgtgccgt cgcgcggaac cagtgggccc gaccgctccc ccgacagtag agcgcctgcg
     4321 tcgacgccca gcagatgagc ggccaaggcc gggtcagcga gatggtgctt gggcgcctgc
     4381 gtcagccgat ggagccggtt atgactcggt agccacgccg gcaccgggtc aagcagccat
     4441 agctgggcca gcacgtcgcg gtaggcgatc gtcgtggtct tggccggctt gtccgaccgc
     4501 ccgggcgtcg cggcgtcaag gatggcgtta tagctggcgg tcgtcgcggt cgccgcggcg
     4561 taggccgtca gccagccgcg caacgtctcg ggtcgacgta cgcgcagccc ttgctcaggg
     4621 aactcatgct cgacgacgcg gctgaggtag ccggccagct gcgcgcgccg ggcacgaccg
     4681 gacagggtgc ggatggcggg cagcccggag gcgaggatct gctcgacgta gtcgtcgagg
     4741 ccgacgtcgc aagatccctc gatggcggcc cgatgtccgt cgagcagacg cgccaggctc
     4801 actgtgggct cagcgaggcc gcgctcgggc aggctgagcg gccgcatccg caccgagacg
     4861 atccggccgg cccccgagtg gaccgcggcg tgtgcgggca cggcgctgcc agtgagcaga
     4921 aagcgaccgg gcggggcacc tgcgtcgacc cggcgcctga tgaagtccca caccggcgga
     4981 tggcgttgcc actcgtcaag cagcaccgtt ccgggcaccg cgtcgagctg ggaggggtct
     5041 gcgacaacaa cctcaagcgt cgccgggtcg tcgagggcca tcactcgctg tgcccggcgg
     5101 ctcgcggtga cggtcttgcc cacgcccttt ggaccctcga ggaccacggc ggagagctcg
     5161 ctcatgagct cgtcaagctc gtcatctacg gtgcggggca ggtagtggtc caccgtctta
     5221 ggctaccgtt tcaaccgcat ctacgctacc gtttcaacac tacgtactct accgtttcaa
     5281 cattgacgcg atcgtgcggt gagcgccggc gtcaggcgtt gccggcgcgg atgccggcca
     5341 gccaagcctg cgcgtcagcg aactctgccc ccgaggtgcc cgggcgcacg gtcggggcga
     5401 ccttgtcggc gcgcgggtag gagccgagga accgcacgtc ggcgcagacc cggcgcagcc
     5461 ccatcaacgc ctccccgacc cgggcgtcgg ccacatggcc ctcgcagtcg atcgagaagt
     5521 ggtagtcgcc gagccggtca ccggtcgggc gcgactcgat ccgggtcagg ttgaccccgc
     5581 ggacggcgaa ctcggtgagg atctcgagca gcgcgccggg ccggtcgtcg cggatgtagg
     5641 cgaccaccgt cgtcttgtcg gtgccggtcg gcgcgggcgg cgcggccggc cggccgacca
     5701 gcacgaaccg ggtgactgcc cgccggttgt cggcgatgtt gtcggcgagc acctcgagcg
     5761 ggtagtgctc gaccgcgacc ggggcggtca gagcggcgtc gtacgacgac tcgccggcgg
     5821 ccaccgccgc cgcggccgcc gccgtcgacg tggccgcgat caacgccgcg tcgggcagcg
     5881 tgcgggccag ccagcgccgg cactgggcca gcgcgtgcgg gtgcgtgccg acgccgcgga
     5941 ccgccgacag cgcggtgccc gggcgggcca gcagggcgaa cgccaccggc aggtgcacct
     6001 cgcgggtgac catcagcggg tcgccggtgg ccagctcgtc gagggtggcg gtgaccgcgc
     6061 cctcgacgga gttctcgatc ggcaccatcg cggcggccac ctcgccgcgc cgtacgccgt
     6121 caagcgccga gggcaccgtc ggataggcga tcagctcggc ctcggcggcc gccggcaggg
     6181 tgcgcagcgc cgactcggtg aaggttccct cgggcccgag gtagccgaag cgcgcgacga
     6241 tcacggcggt cggctcggtc acggcgacgg ggtcgggcac ggcggactcg gtcacggtgt
     6301 cggggtcggt cacggtgtcg gggtcagcgg acggcggcat ggccgggcgc gctctcgagg
     6361 gcaaacgcga tggcctgctc ctcctcgggg gacaacgcct ggtcgctgcg tccgccgcgc
     6421 agccccttgg cgtaggtgcg ggtgtcgcgc cgaccggtga tcgtggtgag caccagcccg
     6481 tcgccgtcgt cgtcgagcag ggcgaccgag aacgacatcc gcccgcccat gtcgctgaac
     6541 gcgtcgtagc ggaccacggc gacgtggcgg atcgcctctt cgagcgcctg gcgggtgtcg
     6601 gcgttctcgc ggcgggcggc gtgcacctcg gcccgcagcg cctcaaccgc gtcgatctgc
     6661 cgctcgaccg cggtgacgaa gtcgtcgctc cggccatccc cctgcagcag gtcgtaagac
     6721 cgacgcagcc gcccaatccg ccggttggcg accagcgcga acgtcagccc gagcaggccg
     6781 agcgcgacgc cgccgatggc gagcaggtca agggtctggg ggtccagcgg cacgcgcaca
     6841 gactacggct ctcacttgct gcggcccgcg atactcgccg ggggccacca gccttcggcg
     6901 tacggcgagc gggacggcgt acggcgagaa ggagacggcg tacgacgacc ggctactgtc
     6961 gcggtcgtgg atcgcgagga cgtcgcggcg ctgctcgacg cggtcgccac cggccaggtc
     7021 agcaccgagg cggcgctggc gcggctggcc gccggccccc tcgacggcgg cccgcttggc
     7081 gacgggttcg ccgacctggg ctacgcccgg ctcgacaccc accgggcgct gcgcaccggc
     7141 gaccccgagg tggtctacgg cgccggcaag acacccgccc agacggtcga gctgctgcgc
     7201 gcgctgcgct cggcccaccc caaccggccg gcgctggcca cccggctgcc cgccccggcc
     7261 cagggcgcgg tgcgcgcggc gttccccgac gcgcagatcg accccagcgc ccgtacggcg
     7321 gtggtcggcc cactgccctc gccgcgaggc accgtcgtgg tggtggccgc cggcacctcc
     7381 gacgccgcgg tggccgccga ggccgccacg acggcacgcg cgtttggcgc cggcgtcacg
     7441 cgccttgatg acgtcggggt ggccggcatc caccggctgc tcgccgcccg ggccaccctc
     7501 gacaccgccg actgcctgat cgtggtggcc ggcatggagg gcgcactgcc cagcgtggtc
     7561 ggcgggctga ccggggtgcc gctggtcgcg gtgcccacca gcgtcggcta cggcgcctcg
     7621 ttcggcgggc tggccgcgct gctcgggatg ctcaacgcct gcgcgcccgg ggtcgtggtc
     7681 gccaacatcg acaacgggtt cggcgccggg gtgttcgcgg cccgggtggc ccgccgggcc
     7741 gctccacgga cgagcgcccc cgcgaccgac ccggcttcga ccgacgcaat cgcccccgcc
     7801 acgaacgagg ccggcgcgac gtgacccgga tcggctggat cgacgccacc gtgggtgcgg
     7861 ccggcgacat gctcgccgcc gcgctggtcg gggccggggc gtcgtacgac gtgatggccg
     7921 aggccgtcgc cgccgtcggc gtacccgtca ccaccgccat cacaaccgtt gacgcacaag
     7981 ggcttgcggc ctggcgctgg agcgtgcgaa ccgacgagac cgacccgccg cggcggacct
     8041 gggccgacat ccgcgcgcgc ctcgacgccg ccgacctcga cccggcggtg cgggcgctgg
     8101 cgcacgacgc gtttgagcgg ctggcca
//
LOCUS       DATIBB010000033         7737 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_672052, whole genome shotgun sequence.
ACCESSION   DATIBB010000033 DATIBB010000000
VERSION     DATIBB010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 7737)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7737)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7737
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_672052"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..272
                     /locus_tag="VLC50_01135"
     CDS             <1..272
                     /locus_tag="VLC50_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018680856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HSF26097.1"
                     /translation="RAVEHGRTVLSASTTGISAIIAPDGAVLASTEVFTADTLVRSVP
                     LRTATTPADRVGAWPELVLTALGLAALTAGVASRLTARRRAGWRG"
     gene            269..1045
                     /locus_tag="VLC50_01140"
     CDS             269..1045
                     /locus_tag="VLC50_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014146097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HSF26098.1"
                     /translation="MSTTAPREPVAADRVLVVIPTYDERDNLAPIVARVRAAVPQAHL
                     LVVDDASPDGTGELADALAREDTQVHVLHRAGKAGLGAAYLHGFSWGFDHDFQVLVEM
                     DADGSHQPEQLPALLEALADADLVLGSRWVPGGEVVNWPTRRRLISRGGTTYARLALG
                     VPLHDITGGFRAFRRTTLEALDLHDVASQGYCFQVDLAWRAVRRGLRVVEVPITFVER
                     ERGASKMSGAIVREALVRVTGWGLAHRAEQLRALRRRNQT"
     gene            1042..1431
                     /locus_tag="VLC50_01145"
     CDS             1042..1431
                     /locus_tag="VLC50_01145"
                     /inference="COORDINATES: protein motif:HMM:NF008528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HSF26099.1"
                     /translation="MIAALLLAFLIVPIVEIAVIIEVGSWIGVVPTVVLLLVESLLGA
                     WIVKHEGLRTWRALRAAVTEGRLPDRELADGALVLVGGALLLAPGFVTDLAGFFLVLP
                     LTRPLARAGLLWLAVRRAPVVRVVTRR"
     gene            1431..1898
                     /locus_tag="VLC50_01150"
     CDS             1431..1898
                     /locus_tag="VLC50_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018798407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HSF26100.1"
                     /translation="MCSYCGCRAIPTIADLTAEHEEIAYVAGELRRALLRGDAAQARS
                     RIQALRDLLHPHNTVEEVGIYPAMARQAEYAEAVATLFDEHDEVDAVLAQALDPDADL
                     APGPILAALELLTRHVDKEENGLFPAAAVVFDPADWAAAEQARADLVPPALSP"
     gene            complement(1935..2309)
                     /locus_tag="VLC50_01155"
     CDS             complement(1935..2309)
                     /locus_tag="VLC50_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014134296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein RbpA"
                     /protein_id="HSF26101.1"
                     /translation="MGERALRGSRLGATSYESDDGASVAERQEITYDCPMGHRFTVPF
                     SVEADVPPVWECRACGAEALRVDATRPEPKKTKPVRTHWDMLLERRSIAELEEVLAER
                     LEVLRSAGGLTPPRSRRQRKSA"
     gene            complement(2403..3791)
                     /locus_tag="VLC50_01160"
     CDS             complement(2403..3791)
                     /locus_tag="VLC50_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSF26102.1"
                     /translation="MRGPATDTPGYPPLAQRRREQRAWYVYDWANSAYVTTIGTVLFA
                     PYLTSVAETAACGAPGTLEAPCRATLTVLGLPVSPGSLAFYVVTVSTIFSALVLPVVG
                     ALADRSARKKHLMGAFAWTGAGAAMAMVTVTDGHWLRGAALLTLGNVCLGMSLVVYDA
                     ILCEIATPDERDAVSSHGWALGYLGGGLLLVLNLALVLGHDSLGVDKGTAVRLSLFSA
                     GLWWAVFTLVPVVGLRNRAPVNPVPQSGSLLGQGFGQLWTTLRELRAYPMTLLFLLAY
                     LFFNDGIQTVIVAASVYGEAALGHPQEVLIGTILLVQFVAFGGALLFGRLAGRWGSRR
                     MIMAGLVCWIGVVTIGFVLPARQVLPFLGLAVLIGIVLGGTQALSRSLYSQLVPKGRE
                     AEYFSLYQAGERGTSWLGTALFGLVHQLTGSYRPAIVALMVFFIVGLALLARVDVRRG
                     IAEAGNAAPAVV"
     gene            complement(3820..4572)
                     /locus_tag="VLC50_01165"
     CDS             complement(3820..4572)
                     /locus_tag="VLC50_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018490160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HSF26103.1"
                     /translation="MAHRGGAHLPGNEGLENSLAAFANAVALGYRYLETDVHATADGV
                     LLAFHDHHLDRVTDGRGRVGDLPWRVVREARIGGTEPIPTLAELLDAFPEARVNVDVK
                     HRSAVAPLVDAVRRTGAIDRVCVASFSDRRLAAVRRALGPRLCTSYGPRDATTLRLLA
                     ALPTDSVSVARAVLPAGVPCAQLPHAVGRLPVTTARLVALAHRAGVSVHVWTVDDPVR
                     MRALLDLGVDGLITDRPDTLRDVLRERGLWER"
     gene            4668..6827
                     /locus_tag="VLC50_01170"
     CDS             4668..6827
                     /locus_tag="VLC50_01170"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HSF26104.1"
                     /translation="MAVTSGGTRGRGLLLTLDRRTPIVVKVAIPVLAIVLGLALFLGT
                     EVADMVSLPCSGSADCPRTKAASIALVAAYDSFLLLGVLGLVFWFVVLRPVHRIAAVA
                     RRVAAGDLDGRVGSRGTGPARDALHAVAGEFDHMLDTLREERAQLLAAQRRYQTILQT
                     ATDAFISVDNAGRVTEWNARAESMFGWPRREMLGRPLGDAIVPVHWRAAHEAAFHYVM
                     TQMDDPLVGKPVEAAALRRDGTTLPVELTVWHSDVDGQPHLNAFLRDITERKQMEERL
                     RHQAFHDGLTGLANRALFANRVGHALARRPDARRGIAVIFLDLDDFKGVNDTLGHSGG
                     DEMLVEVGRRLSGCVRDGDTLSRFAGDEFAVLIEDVEDEAPAVATAERLISALTAPVD
                     IAGRRVATRASIGIAWAPCGEGVRADEVLRDADVAMYAAKRHGKATYEIFDPAMHALE
                     VHRSQRRADLLLALDRGQLVVQYQPYLNAATGVLAGFEALVRWTHPREGLLPPDEFVP
                     LAEESGLIREIDLWVLRQACERAATWAREHPEHPPTTMSVNVSASALHGATLVDDVVE
                     VLRDTGLPASRLVLEITETVMMADVEAAALRLAELKALGIRLAIDDFGTGYSSLSYLQ
                     SLPVDIVKIDRSFVGRLGQEPDQAALVNSVLELGRVLGLQSVVEGVESPEQMAELERL
                     GADIVQGFLISRPVDAIDAEDFLTEAPHPLVGRLTSS"
     gene            complement(6859..>7737)
                     /locus_tag="VLC50_01175"
     CDS             complement(6859..>7737)
                     /locus_tag="VLC50_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020391294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4173 domain-containing protein"
                     /protein_id="HSF26105.1"
                     /translation="LSVDNFVLHAFVSVVVAGTVLAAAYLALNPPQVDRAGHGARPVS
                     HRFEWLAPVLVVIGVFAAFLAAQATVIFGGHDYLRRTTGLTYADYVHQGFGQLTVATA
                     LTLLVIWAAARKAPRATPSDRAWLRLSLGTLCLLTLVVVASALHRMHVYQEAYGFTQL
                     RLLVDVFEGWLGLLVLAVLAGGIALRAAWLPRFALLSGVVALLVLTAINPEAWIAQQN
                     LDRYTATGRVDWAFMQSLSDDAAPTLAALPDQLRGCALTGRDAASDDWLEWNLGRARA
                     AGVLAGNAHGGAAACP"
BASE COUNT         1100 a         2812 c         2827 g          998 t
ORIGIN      
        1 tgcgcgccgt cgagcacggc cgcaccgttc tgtcggcctc gaccaccggg atcagcgcga
       61 tcatcgcgcc ggacggggcg gtgctcgcct ccaccgaggt cttcaccgcc gacacgctcg
      121 tgcgctcggt gcccttgcgc accgcgacca cgccggccga ccgggtaggg gcctggcccg
      181 agctggtgct cacggcgctc gggctggcgg cactgacggc cggggtggcg tcccggctga
      241 ccgcccggcg cagggcagga tggcgcgggt gagcaccacc gccccgcgcg agcccgtcgc
      301 cgccgaccgg gtgctcgtgg tgatcccgac gtacgacgaa cgcgacaacc tcgcaccgat
      361 cgtcgcccgc gtccgggcgg cggtgccgca ggcgcacctg ctcgtcgtcg acgacgcctc
      421 accggacggc accggcgagc tggccgacgc gctcgctcgc gaggacaccc aggtgcacgt
      481 gctgcaccgg gccggcaagg cgggcctcgg cgcggcctac ctgcacgggt tcagctgggg
      541 cttcgaccac gacttccagg tcctcgtcga gatggacgcc gacggctcgc accagcccga
      601 gcagctgccg gccctgctcg aggcgctggc cgacgccgac ctggttctgg gctcgcgctg
      661 ggtgcccggc ggggaggtgg tcaactggcc gacgcgccga cgactgatct ctcgcggcgg
      721 cacgacctac gcccggctgg cgctcggggt gccgctgcac gacatcaccg gcggcttccg
      781 ggcctttcgc cgtacgacgt tggaggccct cgacctgcac gacgtggcca gccagggcta
      841 ctgcttccag gtcgacctgg cctggcgcgc ggtccgccgc gggctgcggg tcgtcgaggt
      901 cccgatcacg ttcgtcgagc gggagcgcgg ggccagcaag atgtccggtg cgatcgttcg
      961 tgaggcactt gtgcgggtga ccgggtgggg gctggcccac cgcgcggaac aactgcgcgc
     1021 tctcagacgt cggaaccaga cgtgatagcc gcgctgctgc tggccttcct catcgtcccg
     1081 atcgtcgaga tcgcggtgat catcgaggtg ggcagctgga tcggtgtcgt gccgaccgtg
     1141 gtgctgctgc tggtcgagag cctgctcggt gcctggatcg tcaagcacga ggggctgcgc
     1201 acctggcggg cgctgcgggc ggcggtgacc gaggggcgac tgcccgaccg cgagctggcc
     1261 gacggggcgc tcgtgctcgt gggcggcgcg ctcctcctgg cccccggctt cgtcaccgac
     1321 ctggccgggt tcttcctcgt gctgccgttg acacgcccgc tggcccgggc cgggctgctg
     1381 tggctggcgg tccgccgggc gccggtcgtg agggtggtga cccgccgctg atgtgcagct
     1441 attgcggctg ccgggccatc ccgactatcg ccgacctcac cgccgagcac gaggagatcg
     1501 cctacgtcgc cggtgagctg cgccgcgcgc tgctgcgcgg cgacgcggcg caggcccgct
     1561 cgcgcatcca ggcgctgcgt gacctgctgc acccgcacaa cacggtcgag gaggtcggca
     1621 tctacccggc gatggcccgc caggccgagt acgccgaggc ggtggccacc ctcttcgacg
     1681 agcacgacga ggtcgacgcg gtgctggccc aggcgctcga ccccgacgcc gacctggcgc
     1741 ccggaccgat cctggccgcg ctcgagctgc tgacgcgcca cgtcgacaag gaggagaacg
     1801 ggctgttccc ggcagccgcg gtcgtcttcg acccggccga ctgggcggcg gccgagcaag
     1861 cccgcgctga cctggtccct cccgcgctca gcccttgact tgaggtgccc tcacggctca
     1921 gacgcccttg cggctcaggc gctcttgcgc tgccgacgcg accgcggcgg ggtcagaccg
     1981 ccggccgacc ggagcacctc gagccgctcg gccagcacct cctcgagctc ggcgatggag
     2041 cgccgctcga gcagcatgtc ccagtgggtg cggaccggct tggtcttctt gggctcaggc
     2101 cgggtcgcgt ccacgcgcag cgcctcggcg ccgcacgcgc ggcactccca caccggaggc
     2161 acgtcggcct cgacggagaa gggcacggtg aaacggtgcc ccatcgggca gtcgtaggtg
     2221 atctcttgac gctcggccac gctggcgccg tcatcgctct cgtagctcgt ggcgcccagc
     2281 cgtgatccgc gcagcgctcg ctcgcccatg agtgatcctc cgtccattca tcaagaccaa
     2341 cggccgggcg cgcaccggtg ttccaccggt cggcggatcc tcagcgaact ctcagggtgg
     2401 gctcacacca cggccggagc cgcgttgccg gcctcggcga tgccccggcg gacgtcgacc
     2461 ctggccagca gggccaaacc gacgatgaag aagaccatga gcgcgacgat cgccggccgg
     2521 taggagccgg tgagctggtg gaccagcccg aacagggcgg tgccgagcca ggaggtgccc
     2581 cgctcgccgg cctgatagag gctgaagtac tccgcttcgc gccccttggg caccagctgg
     2641 ctgtagagcg agcgggacaa cgcctgggtg ccgcccagca cgatgccgat gagcacggcc
     2701 aacccgagga acggcagcac ctggcgggcc ggtaggacga acccgatggt caccacgccg
     2761 atccagcaca ccagcccggc catgatcatg cggcggctgc cccaccggcc ggccagccgg
     2821 ccgaacagca gcgccccacc aaaggcgacg aactgcacga gcaggatcgt cccgatcagc
     2881 acctcctgcg ggtgcccgag cgccgcctcc ccgtagacgc tggccgcgac gatcacggtc
     2941 tggatgccgt cgttgaagaa gaggtaggcg agcaggaaca gcagcgtcat cggataggcg
     3001 cgcagctcac gcagggtggt ccacagctgt ccgaagccct gcccgagcag cgaccccgac
     3061 tgcggcaccg ggttgaccgg cgcccggttg cgcagcccaa ccaccggcac cagcgtgaac
     3121 accgcccacc acaggccggc cgagaacagg ctcaggcgta cggcggtgcc cttgtccacg
     3181 ccgaggctgt cgtgaccgag gaccagggcg aggttgagca ccagcagcag gccacccccg
     3241 aggtagccca gcgcccagcc gtgcgaggac accgcgtcgc gctcgtcggg ggtggcgatc
     3301 tcgcaaagga tcgcgtcgta gaccaccagc gacatgccca ggcagacgtt gccgagcgtg
     3361 agcagcgccg cgccgcgcag ccagtggccg tcggtgacgg tgaccatcgc catcgcggcg
     3421 cccgcgccgg tccaggcgaa ggcccccatc aggtgcttct tgcgcgcgct gcggtcggcc
     3481 agcgcgccca cgaccggcag cacgagcgcc gagaagatcg tcgagacggt cacgacgtag
     3541 aacgccagcg acccggggga gaccggcagg ccgagcaccg tcagggtggc ccggcagggc
     3601 gcctcgaggg tgcccggtgc cccgcaggcc gcggtctcgg cgaccgaggt caggtagggc
     3661 gcgaacagca ccgtgccgat ggtcgtgaca taggcggagt tggcccagtc gtagacgtac
     3721 caggcgcgct gctcgcggcg ccgctgggcc agcggcgggt agcccggcgt gtcggtggcc
     3781 ggcccgcgca tggtcgtcat gcgcgcacat cctgccgcct cagcgctccc agagcccccg
     3841 ctcgcgcagc acgtcgcgca gcgtgtcggg gcggtcggtg atcagcccgt cgacgcccag
     3901 gtcgagcagc gcccgcattc ggaccgggtc gtcgacggtc cagacgtgca cgctcacccc
     3961 ggcccggtgc gccagcgcga ccagccgggc cgtggtcacc gggagccgcc cgaccgcgtg
     4021 ggggagctgc gcgcacggca cgccggccgg cagcaccgcc cgggcgaccg agacgctgtc
     4081 ggtgggcagc gcggccagca gccggagcgt ggtcgcgtca cgaggcccgt acgacgtgca
     4141 cagccgcggc cccagcgccc gccgtaccgc cgccagccgc cggtccgaga acgacgcgac
     4201 gcacacccgg tcgatcgccc cggtgcgccg tacggcgtcc acgagcggag ccaccgccga
     4261 ccggtgcttg acgtcgacgt tgacccgggc ttccgggaag gcgtcgagca gctcggccag
     4321 ggtcgggatc ggttcggtgc cgccgatgcg tgcctcccgc accacccgcc acggcaggtc
     4381 gccgacccgc ccccggccgt cggtgacccg gtcgaggtgg tggtcgtgga aggccagcag
     4441 caccccgtcg gccgtggcgt gcacgtcggt ctcgaggtag cggtagccga gcgcgaccgc
     4501 attggcgaag gcggccaggg agttctcgag gccctcgttg cccggcaggt gggcgccgcc
     4561 gcggtgggcc atcgcgatcg gcccgtcgtg ctcgaggaac gcgaaggccg ccacgcccac
     4621 gagtattgcc actcggggct cagtcccacg caaaacgtgc cgatggtatg gccgtgacca
     4681 gcgggggaac acgtgggcgc gggctgctcc tgaccttgga ccgtcgtacg ccgatcgtcg
     4741 tcaaggtcgc catccccgtg ctcgccatcg tcttggggct ggccctgttc ctgggcaccg
     4801 aggtggccga catggtctcg ctgccctgct cggggtcggc cgactgcccc cgcaccaagg
     4861 ccgcctcgat cgccctggtg gccgcctatg actcgttcct gctgctcggc gtgctcgggc
     4921 tggtgttctg gttcgtcgtg ctgcggccgg tgcaccggat cgccgcggta gcgcgacggg
     4981 tcgccgcggg cgacctggac gggcgagtcg gcagccgcgg caccggcccg gcccgcgacg
     5041 cgctgcacgc cgtggccggc gagttcgacc acatgctcga cacgctgcgc gaggaacggg
     5101 cccagctgct cgccgcacag cgccgctacc agaccatcct gcagaccgcc accgacgcgt
     5161 tcatcagcgt ggacaacgcc gggcgggtca ccgagtggaa cgcccgggcc gagtcgatgt
     5221 tcggctggcc ccgccgggag atgctgggcc ggccgctggg tgacgcgatc gtgccggtgc
     5281 actggcgggc cgcccacgag gcggcgttcc actacgtgat gacccagatg gacgacccgt
     5341 tggtcggcaa gccggtggag gcggccgcgc tgcgccggga cggcaccacg ctgccggtcg
     5401 agctgaccgt ctggcacagc gacgtggacg ggcagccgca cctcaacgcg ttcctgcgcg
     5461 acatcaccga gcgcaagcag atggaggaac ggctgcggca ccaggcgttc cacgacgggc
     5521 tgaccgggct ggccaaccgg gcgctgttcg ccaaccgggt cggtcacgcg ctggcccgtc
     5581 ggcccgacgc acggcggggg atcgcggtga tcttcctgga cctcgacgac ttcaagggcg
     5641 tcaacgacac gctcggccac agcggcggcg acgagatgct ggtcgaggtg ggccggcgac
     5701 tgtccggatg cgtgcgggac ggagacaccc tctcccggtt cgccggcgac gagttcgccg
     5761 tcctgatcga ggacgtcgag gacgaggcac ccgcggtggc caccgccgag cggctgatca
     5821 gcgcgctgac cgcgccggtc gacatcgcgg gacgccgggt cgccacccgg gccagcatcg
     5881 gcatcgcctg ggcgccgtgc ggcgagggcg tgcgcgccga cgaggtgctg cgtgatgcgg
     5941 acgtggcgat gtacgccgcc aagcggcacg gcaaggcgac ctacgagatc ttcgacccgg
     6001 ccatgcacgc cctggaggtg caccgcagcc agcgccgggc agacctgctg ctggcgctgg
     6061 accggggcca gctggtcgtg cagtaccagc cctacctcaa cgccgcgacc ggtgtcctgg
     6121 ccgggttcga ggcgctggtg cgctggaccc acccgcgcga gggcctgctg cccccagacg
     6181 agttcgtccc gctggccgag gagtccggcc tcatccggga gatcgacctg tgggtgctgc
     6241 gccaggcctg cgaacgagcc gccacctggg cgcgtgagca ccccgagcac ccgccgacga
     6301 ccatgagcgt caacgtgtcg gcctcggcgc tgcacggcgc caccctggtc gacgacgtgg
     6361 tcgaggtgct gcgcgacacc ggtctgccgg cctcccggct cgtgctcgag atcaccgaga
     6421 ccgtgatgat ggccgacgtg gaggctgcgg ccctccggct ggccgagctc aaggcgctcg
     6481 gcatccggct ggccatcgac gacttcggca ccggctactc gtcactgagc tacctgcagt
     6541 cgctgccggt ggacatcgtc aagatcgacc gaagcttcgt gggccggctg ggccaggagc
     6601 ccgaccaggc cgcgctcgtc aacagcgtgc tcgagctcgg ccgggtgctc gggctgcaga
     6661 gcgtggtcga gggcgtggag tcaccggagc agatggccga gctggagcga ctcggcgccg
     6721 acatcgtcca gggcttcctg atctctcgcc cggtcgacgc catcgatgcc gaggacttcc
     6781 tcaccgaggc gccgcatccg ctggtcggcc gcctgacctc gtcctgacga ccgcctgttc
     6841 cgccggggcg gggtcccctc agggacacgc ggccgcccca ccgtgggcgt tgccggcgag
     6901 gacgccggcg gcccgggccc ggcccaggtt ccactccagc cagtcgtcgg aagccgcgtc
     6961 cctaccggtc agggcacagc cgcgcagctg gtcgggaaga gcggccagag tcggcgcggc
     7021 gtcgtcggac aggctctgca tgaaggccca gtccacccgc ccggtcgcgg tgtagcggtc
     7081 gaggttctgc tgcgcgatcc acgcctcggg gttgatcgcc gtgaggacca acagggcgac
     7141 caccccgctg agcagggcga accggggcag ccacgcggcc cgcagcgcga tcccgccggc
     7201 cagcaccgcc agcacgagca ggccgagcca gccctcgaag acgtcgacga gcagccgcag
     7261 ctgggtgaag ccgtaggcct cctggtagac gtgcatgcgg tgcagggccg aggcgactac
     7321 gaccagggtc agcaggcaca gcgtcccgag agacagccgg agccaggccc ggtccgaggg
     7381 ggtggcgcgg ggcgccttgc gggcggccgc ccagatgacc agcagggtca gggcggtggc
     7441 gacggtgagc tggccgaacc cctggtgcac gtagtcggcg taggtcaggc ccgtcgtacg
     7501 acgcaggtag tcgtgaccgc cgaagatcac cgtggcctgg gccgccagga acgcggcgaa
     7561 gacgccgatc acgacgagca ccggggccag ccactcgaag cggtggctca ccgggcgcgc
     7621 gccgtggcct gcgcggtcga cctggggcgg gttgagcgcg aggtaggccg ccgccagcac
     7681 cgtgccggcg acgaccaccg agacgaacgc gtgcagcacg aagttgtcca cgctcag
//
LOCUS       DATIBB010000034         2897 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_804050, whole genome shotgun sequence.
ACCESSION   DATIBB010000034 DATIBB010000000
VERSION     DATIBB010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2897)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2897)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2897
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_804050"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..308)
                     /locus_tag="VLC50_01180"
     CDS             complement(<1..308)
                     /locus_tag="VLC50_01180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF26106.1"
                     /translation="MFDNTGDGPADTSDPTPADVGPALPGLAGRLLAGLDPASAAAVR
                     AAVSHEQVPVDEDVSLPAWAGLPGSLALAIAVAELDPADLDEPARVELIAAYERLAGW
                     V"
     gene            445..1314
                     /locus_tag="VLC50_01185"
     CDS             445..1314
                     /locus_tag="VLC50_01185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF26107.1"
                     /translation="MAAGRHERPPHLRQLAPIAAAVLLAALALVLSPIGGDRWLRSLA
                     AVAVLVVALPGMAALDGLLRVSDRAAEQATARAEEAARLRQQVAELRREYDAGRVSTA
                     ETLDNVQRDLAATRAYARGGRDELVTAVERISALERGLSAARTEVGEVSARLQDAAAD
                     AIAARSDLADMRSEVSGTRAEVADTRAEVADTRAEVAAANEVVLAARAEAATSASTVS
                     EVAEGLRVQLGALRTELAALRAELDARAEGVEATTVRMTLPLVRAALRHDPLQPATPA
                     PSRLGERPRRTGS"
     gene            1396..1896
                     /locus_tag="VLC50_01190"
     CDS             1396..1896
                     /locus_tag="VLC50_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020549583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HSF26108.1"
                     /translation="MGSASDDVFSLPDVAFRPVSPRLVPMRRTAYVLLIVPVTVGLAL
                     LLGLTVAGWLALLVVGLAVVALGWGWVALGRTARSWAYAERADDLLIVHGVLIRRLVV
                     VPYGRMQLVDVHVGPLDRAFGLARVQLHTAAATTDAHIPGLEPAEATVLRERLAALGE
                     ARSAGL"
     gene            1893..>2897
                     /locus_tag="VLC50_01195"
     CDS             1893..>2897
                     /locus_tag="VLC50_01195"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HSF26109.1"
                     /translation="MTERSRRLHPLSPLVRGWKALAVLAAIGAQQGLGSGGPGRGLPS
                     VGIVAAVIVVVLVGGLAAGYLSWRFTRYEIDGDTLRVDTGLLFRRTRVARLDRLQAID
                     VIRPVVARALGLAELRLESAGGGDSHVPLAYLAEDEAYALRAELLARAAGIAADTPAA
                     PERVLAEVSLPDLLVSRVLTLGFLLGLGATLLLGAVAIVGGEVSLLAAVLPAAAASIL
                     PAGQQLLTHYGFTVADSPDGLRIRHGLLETSAQTVPPGRVQAVSVVEPLLWRRMRGWC
                     RVEINVAGYAGAQAASETVLLPVGSRRTALEILARVLPGLDLDAIPLRPAPARARWCD
                     P"
BASE COUNT          308 a         1081 c         1104 g          404 t
ORIGIN      
        1 acccagccgg cgaggcgttc gtaggcggcg atcagctcaa cgcgggcggg ctcgtcgagg
       61 tcggccgggt ccagctcggc gaccgcgatg gccagggcca ggctgcccgg cagacccgcc
      121 cacgccggca ggctcacgtc ctcgtcgacc gggacctgct cgtggctgac cgcggcgcgg
      181 accgccgccg ccgaggccgg gtccagcccg gcgagcagcc gccccgccaa ccccggcaac
      241 gccggaccca cgtcggccgg cgtcgggtcg ctggtgtctg ctggtccatc ccccgtgtta
      301 tcgaacatgt gttcgagtct agcggagaga gccgtcaaat ggaagcatca agacgcaact
      361 gatgtgttct tagaggattc ggccttcaac gcgggcccgc gcgcctccgg cgtcccgcgt
      421 agcgcacgcc gtaccctttc gatcatggca gcaggccggc acgagcgacc cccgcacctg
      481 cgccagctgg ccccgatcgc ggcggccgtg ctgctggcgg cgctcgctct tgtgctcagc
      541 ccgatcggcg gcgaccggtg gctgcgctca ctcgccgccg tcgccgtcct cgtcgtcgcc
      601 ctgcccggca tggctgctct cgacgggctg ttacgggtct ccgaccgggc cgccgagcag
      661 gccaccgcgc gagccgagga ggccgctcgg ttgcgccagc aggtcgccga gctgcgtcgc
      721 gagtacgacg cggggcgggt cagcaccgcc gagaccctcg acaacgtgca gcgcgacctc
      781 gcggcgaccc gcgcctacgc ccgaggcggg cgagacgagc tggtgaccgc ggtggagcgc
      841 atcagcgcgc tcgagcgcgg gttgagcgcg gcgcgtaccg aggtgggtga ggtgtcggct
      901 cggttgcagg atgccgcggc cgacgcgatc gccgcccgga gcgatctcgc tgacatgcgc
      961 tcggaggtgt cgggaacccg cgccgaggtc gccgacaccc gtgccgaggt cgccgacacc
     1021 cgcgccgagg tcgctgccgc caatgaggtg gtgctcgccg cgcgggccga ggcggccacc
     1081 tcggccagca ccgtctccga ggtcgccgag ggcctgcggg tgcagctggg cgcgctgcgc
     1141 accgagctgg ccgccctgcg ggccgagctc gacgcgcggg ccgagggtgt ggaggccacg
     1201 accgtgcgca tgacgctgcc gctcgtgcgg gccgcgctgc ggcacgaccc gctccaaccc
     1261 gccacgccgg ccccgtcgcg cctgggcgag cgcccccgcc gcaccggttc ctgaccgcgt
     1321 cagccgagtc gctcgccatc caccggcagc gcgcgggaag caagggccgc cggccgggtc
     1381 tgccaggatc gcagggtggg ttctgcctcc gacgacgtct tctcgctgcc cgacgtggcg
     1441 ttccggccgg tctcgccgcg gctggtgccg atgcgccgta cggcgtacgt cctgctgatc
     1501 gtgcccgtga ccgtcgggct ggcgctgctg ctcggtttga ccgtggcggg ctggctggcc
     1561 ctgctcgtcg tcgggttggc cgtggtggcc ctgggctggg gatgggtggc cctggggcgc
     1621 accgcccggt cgtgggctta cgccgagcgg gccgacgacc tgctcatcgt gcacggggtg
     1681 ctgatccgcc ggctcgtggt ggtgccctac gggcgcatgc agctcgtcga cgtgcacgtc
     1741 ggcccgctcg accgggcgtt cggactggcc cgggtccagc tgcacaccgc ggcggccacc
     1801 accgacgcgc acatcccggg gctcgagccg gccgaggcga ccgtgctgcg tgagcggctc
     1861 gccgccctgg gcgaggccag gtcggcgggg ctgtgaccga gcggtcgcgt cggctgcacc
     1921 cgctcagtcc gctggttcgc ggctggaagg cgctggccgt gctcgccgcg atcggcgcgc
     1981 agcagggact gggcagcggt ggccccggcc ggggactgcc cagcgtcggg atcgtcgcgg
     2041 ccgtcatcgt cgtcgtgctg gtcggcgggc tggccgccgg ctacctgtcg tggcggttca
     2101 cccgctatga gatcgacggc gacacgctgc gggtagacac cggcctgctg ttccggcgca
     2161 cccgggtggc ccggctcgac cggctgcagg ccatcgacgt catccgcccg gtcgtggccc
     2221 gggccctcgg gctggccgag ctgcggctgg agtcggccgg cggcggcgac tcccacgtgc
     2281 cgctcgccta cctggccgag gacgaggcct acgcgctgcg ggccgagctg ctggcccggg
     2341 ccgccggcat cgccgccgac accccggccg cccccgagcg cgtgctcgcc gaggtgagcc
     2401 tgcccgacct gctcgtctcg cgggtgctca cgctgggctt cctgctcggg ctcggcgcga
     2461 ccctgctcct cggggcggtg gcgatcgtcg gcggagaggt gagcctgctg gcggcggtcc
     2521 tgccggcagc ggccgcgtcc atcctgccgg cgggccagca gctgctgacc cactacgggt
     2581 tcaccgtggc cgactcgccc gacgggctgc ggatccggca cggtctgctc gagacctccg
     2641 cccagaccgt gccgcccggg cgggtgcagg cggtcagcgt ggtcgagccg ctgctgtggc
     2701 gacggatgcg cggctggtgc cgggtcgaga tcaacgtggc gggctacgcc ggagcgcagg
     2761 cggcctctga gacggtgctg ctgccggtcg gctcccggcg tacggcgctc gagatcctgg
     2821 cccgggtgct gcctggcctc gacctcgacg cgatcccgct ccgcccggcc ccggcaaggg
     2881 ctcgctggtg cgacccg
//
LOCUS       DATIBB010000035         8109 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_805098, whole genome shotgun sequence.
ACCESSION   DATIBB010000035 DATIBB010000000
VERSION     DATIBB010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 8109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8109
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_805098"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            236..2002
                     /locus_tag="VLC50_01200"
     CDS             236..2002
                     /locus_tag="VLC50_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019634795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HSF26110.1"
                     /translation="MEEIVGEALATRLVEWGVDTVFGLPGDGINGLMEGFRRRRDALR
                     FVLVHHEEAAAFMATGYAKATGRLGVCVATSGPGGIHLLNGLYDAKLDHVPVLALTGM
                     QASDVLGSAYQQEVHLDRLFADVAAYNLLVTNPRQMPAVVDLAVRTALARQTVAHLTF
                     PNDLQVAPAAEDPYAHVGPGAPPQSMPLTASAELTPRQDDLARAAAVLNAGRAVVMLV
                     GEGARGASADVVAVADRLGAPIVKTLKGKAVVPDEHPLTTGGIGLLGTRPSEEAMENA
                     DTLLMVGTSFPYATFLPEPGKVRAVQIDVDATRIGMRVPVEAPVVADSARALRALLPM
                     LEPASERGFLRRAQEGMTKWRADMAAMVDPKRSPIAPEYLMGCLDEVAAEDAILTCDS
                     GTVATWAARHWNIRAGRQLYLSGNLATMACGLPYAIGMQSAFPDRQVIAYVGDGGFAM
                     LMAELLTAVRHELPLTVIVNNNNSYGMILWEQLLLGFPEYAVRHRPPVADFAAWARAC
                     GAHAEKVSEAAGLKEAVRVALAHPGVALVDVDVDPNEPPMPGKVTYQQAKAVAEAFLH
                     GQPHKREMVAAVARDTIAQMFG"
     gene            complement(2013..2375)
                     /locus_tag="VLC50_01205"
     CDS             complement(2013..2375)
                     /locus_tag="VLC50_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011751575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HSF26111.1"
                     /translation="MTTSPDVRVRRVYDEPASADGARILVDRVWPRGVSKTAARLDEW
                     CKQVAPTTELRRWYAHDPARFAEFERRYRAELTQPEQAAALAHLREMATQGPVTLLTA
                     TREPELSQAAVLATVLGC"
     gene            complement(2372..3529)
                     /locus_tag="VLC50_01210"
     CDS             complement(2372..3529)
                     /locus_tag="VLC50_01210"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HSF26112.1"
                     /translation="MKHRAASWVLAGIGAVVAISVMDLFFDPGINFSGTLAVAPFIPA
                     VAASPLATAGVGLLAIVVGLALFIVDEPPLSVMIAQSAAVILAAVMAPLAARARQRRE
                     RALRDLSRVANVAQRAILAPIAPTVGPVAISAAYLSASRDALIGGDLYAVTKTDRGVR
                     LILGDVRGKGLDAVALAALVVAAFREATRSPTLADVIAVIEDQLRPELGPEDFVTAII
                     AEVGTDGTCTLLSCGHHPPALWRDGVFHVLDEIEPTTPFGLDADPKPHTLRLRNGDRL
                     LFYTDGLVEARAPNGGFIELNSLLAGLGRDALDVVLDRLVLRLRSRAGEITDDLALLL
                     VQFCTTEAKLAPSGATRVPTPRALQSDAPPHRPLQMAHTGLSCEVDEEQWA"
     gene            3635..4399
                     /locus_tag="VLC50_01215"
     CDS             3635..4399
                     /locus_tag="VLC50_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017600090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HSF26113.1"
                     /translation="MAHVTLDPARLDAVVFDMDGVITDTAAVHLAAWARLFDEVLPGL
                     SQPGETPALPFTAEDYRRYVDGVRREDGVARFLASRDIAVAWGSDTDGPEAVTVVGLG
                     RRKNDYFLAELGRSGARAFGSSVALVQSLQARGLGVALVSASRNARVVLDAAGVGTLF
                     AVVVDGTLAARLGLAGKPEPDLFVEAARRAGSRPDRAAVVEDALAGVAAGRAGGFALV
                     VGVDRSGAREALLAAGADMVVEDLAEVDVLEPDVAP"
     gene            4396..6816
                     /locus_tag="VLC50_01220"
     CDS             4396..6816
                     /locus_tag="VLC50_01220"
                     /inference="COORDINATES: protein motif:HMM:NF015588.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HSF26114.1"
                     /translation="MSWLLRYEGFDPATEKLREALCTLGNGRFATRGAAPESVADDVH
                     YPGTYAAGVFNRLSAEVDGHLVDNESMVNLPDWQSLRFRPEDGDWVDLRAVQVSDYVQ
                     ELDLRRGVLTRRFEIEDRQGRRTAVAQRRFVSMHEPVAAGIDSTFVALNWSGRLVVRT
                     GIDGRVSNAGVPRYVGLGSVHLVPVCAEPVDPHTIALDVRTTQSAVQVSLAARARVLA
                     DAAEADVARELVREDGYVAQDLTLDLVEGQPWTVEKVAALYTSRDRAISEPASAARQQ
                     ASNLPGFEDLLAAHVLAWDHRWERCDLTIQAREPDRARASLALHVQLFHVLQTVSEHV
                     VDLDVGIPARGLHGEAYRGHIFWDEMFVYPFLHQRMPELSRSLLLYRWRRLPQARRLA
                     REAGLRGAAFPWQSGSDGREETQAVHLNPRSGRWLPDHSRLQRHINVAVAFSTWQYYE
                     ATADLDFLAEHGAELMLEIAAMWASLATYDRTADRYDIRGVMGPDEFHDAYPWRDEPG
                     IDNNAYTNVMVAWSLERALRCVRILPTRRRRELLDLLGVSTTDLEHWDHVSRKLRLCW
                     HGEGILSQFEGYERLEELAWADYVERYGDIARLDRILEAEGDTTNRYRLSKQADVLML
                     FYLLSADDLAEVLERLGYRYDPMMIPRTIDYYLRRTTHGSTLSKVVHAWVLARSNRTG
                     AWQYLLDALESDLVDTQGGTTPEGVHIGAMAGSIDLLQRAFTGLETRGEQLWLNPVLP
                     DQLTGLQFRLRYREHYGVHVAVDHDRVVVGGSGVGPHRLHLRVRDEELLLDPGGAHEF
                     ALGARDPG"
     gene            6845..7474
                     /locus_tag="VLC50_01225"
     CDS             6845..7474
                     /locus_tag="VLC50_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019818894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HSF26115.1"
                     /translation="MQLTKLGHAALLLEIGQARLLIDPGTYSHGFEDLRGLTGILITH
                     QHADHLDPARIHALVAANPDALVLADAATTAVLAGLGIAATAASAGDTFEPGVLVEVV
                     GQEHAVIHPDIPVVPNVGYLVAERLLHPGDALTVPDRPVELLALPMMAPWMRVADAVD
                     YARAVHPAVVVPIHDALLADPRSYHKWIDQLGPAGTRLAPADGGEPVSF"
     gene            7619..>8109
                     /locus_tag="VLC50_01230"
     CDS             7619..>8109
                     /locus_tag="VLC50_01230"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020133154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HSF26116.1"
                     /translation="MRLLGVPVFLWLIFAEYDGAALVVLACAGASDYLDGLLARRWNQ
                     VSRLGQLLDPAADRLYIFATLVGLSYRDIVPIWLAVVLVSRDAILTACIPVLRRHGYG
                     PLPVHFLGKAATFNLLYAFPLLLLGDGSTVVALLAKTIGWAFAIWGSALYWWAGVLYL
                     VQV"
BASE COUNT         1096 a         2920 c         2918 g         1175 t
ORIGIN      
        1 gctgccgcac ccagatccag caaggggaca ccggacggga ggccgtgcac ctcgcggagg
       61 tcctcgccgg cgcgctcggg cgggcgtaga gcgttggacc gctgcccggg accctcgccg
      121 gctcggccgg cccttccttg ccaggaaagc gccccccggc ctacggtgaa attcgttgag
      181 ccatcagcgc cagatcgacc gggggagtcc ccgtgacgga gacggcggcg cactgatgga
      241 agagatcgtc ggagaggcac tggcgacccg gctggtcgag tggggggtcg acaccgtctt
      301 cggtctgccc ggtgacggca tcaacggcct catggagggc tttcgccggc gccgcgacgc
      361 gttgcggttc gtgctcgtcc accacgagga ggcggccgcc ttcatggcga ccggttacgc
      421 caaggccacc ggccggctcg gcgtgtgcgt agccacgtcg ggtccgggtg gcatccacct
      481 gctcaacggg ctctatgacg ccaagctcga ccatgtcccg gtgctcgcgc tgaccggcat
      541 gcaggccagc gacgtcctcg ggtcggccta ccagcaggag gtgcacctcg accggctctt
      601 cgccgacgtg gcggcctaca acctgctggt caccaacccg cggcagatgc cggcggtggt
      661 cgacctggcg gtacgcacgg cgctggcccg ccaaaccgtg gcgcacctga ccttccccaa
      721 cgacctccag gtggcgccgg cagccgagga cccctatgcc cacgtcgggc ccggggcgcc
      781 gccgcagagc atgcccctga ccgcatccgc cgagctcacc ccgcgccagg acgacctggc
      841 ccgggcggcc gcggtgctca acgccggccg cgcggtggtc atgcttgtgg gcgagggcgc
      901 tcgtggggcc agcgccgacg tcgtggccgt ggccgaccgg ctgggcgctc ccatcgtcaa
      961 gacgctcaag ggcaaggcgg tggtgcccga cgagcatccg ctgacgaccg gtgggatcgg
     1021 gctcttgggc acccgcccga gcgaggaggc gatggagaac gccgacacgc tgctcatggt
     1081 cggcacgtcc ttcccctacg cgacgttcct gccggagccc ggcaaggtcc gcgccgtaca
     1141 gatcgatgtc gatgcgaccc ggatcgggat gcgcgtgccg gtggaggccc ccgtcgtcgc
     1201 cgacagcgcc cgtgcgctgc gagcgctgct gccgatgctt gagcctgcgt ccgagcgcgg
     1261 cttcctgcgc cgggcgcagg aggggatgac gaagtggcgc gcggacatgg cggcgatggt
     1321 cgaccccaag cgcagcccga tcgccccgga gtacctcatg ggttgtctcg acgaggtggc
     1381 ggctgaggac gccatcctca cctgcgactc cggcaccgtc gcgacctggg cggcgcgcca
     1441 ctggaacatc cgggccggtc gccagctcta cctgtcgggc aacctcgcca cgatggcgtg
     1501 cgggctgccc tatgccatcg gcatgcagag cgcgttcccg gaccgtcagg tcatcgccta
     1561 tgtcggtgac ggtggcttcg cgatgctgat ggccgagctg ctcaccgccg tacgtcatga
     1621 gctgccgctc accgtgatcg tcaacaacaa caactcctac gggatgatcc tctgggagca
     1681 gctgctgctc ggcttcccgg agtacgccgt acggcaccgg cccccggtgg ctgacttcgc
     1741 cgcctgggcc cgcgcgtgcg gtgcgcacgc cgagaaggtg agcgaggcgg cagggctcaa
     1801 ggaggcggtg cgtgtcgcgc tggcccaccc gggcgtcgcg ctggtcgacg tcgacgtcga
     1861 ccccaacgag cctccgatgc ccggcaaggt cacctatcag caggcgaagg ccgtcgccga
     1921 ggcgttcctg cacgggcagc cgcacaagcg ggagatggtc gcggcggtcg cccgcgacac
     1981 catcgcgcag atgttcgggt gactcggcct tgtcagcaac ccagcacggt ggcgagcacg
     2041 gccgcttggc tcagctcggg ctctcgcgtg gcggtcagca gtgtcaccgg cccctgggtg
     2101 gccatctcgc gcaggtgagc cagcgcggcg gcctgctcgg gctgggtcag ctcggcccgg
     2161 tagcggcgct cgaactcggc gaagcgcgcc gggtcgtggg cataccagcg acgcagctcg
     2221 gtcgtgggag cgacctgctt gcaccactcg tcgagccggg cggcggtctt gctgaccccc
     2281 cgcggccaga cccggtcgac gaggatccgc gcaccgtcgg cgctggccgg ctcgtcgtag
     2341 acccggcgta cccgcacgtc tggtgatgtc gtcatgccca ctgctcctcg tcgacctcgc
     2401 aggacagccc agtgtgcgcc atctgcaacg gtcggtgagg cggggcgtcg ctctgcagcg
     2461 ctcggggtgt cgggacccga gtggcaccgc tgggggccag ctttgcctcg gtcgtgcaga
     2521 actggacgag cagcagcgcc aggtcgtcgg tgatctcacc agcacgcgaa cgcagccgca
     2581 gcacgaggcg gtcgagcacc acgtcgagcg cgtcgcggcc caggccagcc agcagcgagt
     2641 tgagctcgat gaacccgccg ttgggggcgc gggcctcgac caggccgtcg gtgtagaaga
     2701 gcaggcggtc accgttgcgc agtcggaggg tgtgcggctt ggggtcggcg tccagcccga
     2761 acggggtggt cggctcgatc tcgtcgagga cgtggaacac cccgtcgcgc cacagggccg
     2821 gcgggtggtg cccgcagctg agcagtgtgc aggtgccgtc ggtcccgacc tccgcgatga
     2881 tcgcggtgac gaagtcctcc ggtcccagct cggggcgcag ctggtcctcg atgaccgcga
     2941 tcacgtcggc aagggtgggg ctgcgcgtgg cctcgcgaaa cgcggccacc accagcgcgg
     3001 ccagcgcgac cgcgtctagc cccttgccgc gcacgtcgcc gaggatgagc cgcacaccgc
     3061 ggtcggtctt ggtcaccgcg tagaggtcgc caccgatcaa ggcgtcgcgg ctggccgaga
     3121 ggtaggcggc ggagatcgcg accggaccca ccgtcggggc gatcggggcg aggatcgcgc
     3181 gctgcgcgac gttggcgacc cggctcaggt cacgcagggc ccgctcgcgg cgttggcgtg
     3241 cgcgcgcggc cagcggagcc atgaccgctg cgaggatgac cgccgcactc tgggcgatca
     3301 tgactgacag cggtggctcg tcgacgatga acagtgcgag ccccaccacg atggcgagca
     3361 gcccgacacc ggcggtggcc agcgggctgg cggccaccgc ggggatgaac ggggccaccg
     3421 cgagggtccc gctgaagttg atgcccgggt cgaagaacag gtccatgacg ctgatcgcga
     3481 cgaccgcgcc gatgccggcg agcacccacg aggcggctcg atgcttcacc gggccatcct
     3541 ggcctgaccc gcgcgcgcac ggtgacggaa gtgcgaactc tgtgcttcca ggcaaacccg
     3601 gcttctgtgc ggcactgtgg agcgggaggc cccgatggct cacgtgaccc tcgacccggc
     3661 acgactggac gccgtcgtct tcgacatgga cggcgtcatc accgacacgg cggccgtgca
     3721 cctggctgcc tgggcccggc tgttcgacga ggtgctgccc gggctctcgc agccgggcga
     3781 gacgccggcc ctgcccttca ccgccgagga ctaccggcgc tacgtcgacg gggtccgacg
     3841 cgaggacggg gtcgcgcgct tcctggcctc ccgcgacatc gccgtggcct gggggtcgga
     3901 caccgacggc cccgaggcgg tcaccgtcgt ggggctgggc cggcgcaaga acgactactt
     3961 cctggccgag ctggggcgct cgggtgcccg ggcgttcggc tccagcgtgg cgctcgtgca
     4021 gtcgctgcag gcacgaggcc tgggcgtcgc gctggtgtcg gcctcccgca acgcccgcgt
     4081 cgtgctcgac gccgccgggg tcggcaccct gttcgccgtc gtggtcgacg gcacgctcgc
     4141 ggcccgcctc ggcctggccg gcaagccaga gcccgacctg ttcgtcgagg ccgcccgccg
     4201 cgcgggcagt cggccggacc gggccgcggt ggtggaggac gcgctggccg gggtggcggc
     4261 cggccgggcc ggagggttcg ccctggtcgt cggggtggac cgcagcgggg ctcgggaggc
     4321 cctgctcgcc gccggcgccg acatggtcgt ggaggacctg gccgaggtcg acgtgctcga
     4381 gccggacgtc gcgccatgag ctggctgctg cgctacgagg gcttcgaccc agccaccgaa
     4441 aagctgcgcg aggccttgtg cacgctgggc aacggccggt tcgccacccg cggtgccgcg
     4501 ccggagtccg tcgccgacga cgtgcactat cccggcacct atgcggccgg ggtgttcaac
     4561 cggctgagcg ccgaggtcga cggccacctg gtcgacaacg agagcatggt caacctgccc
     4621 gactggcagt cgttgcgttt ccgcccggag gacggcgact gggtcgacct gcgtgcggtc
     4681 caggtcagcg actacgtgca ggagctcgac ctgcgccgcg gcgtgctcac gcgccggttc
     4741 gagatcgagg accggcaggg gcgccgtacg gcggtcgcgc agcggcggtt cgtctcgatg
     4801 cacgagccgg tcgccgccgg catcgactcg acgttcgtgg cgctgaactg gtcggggcgg
     4861 ctggtcgtgc gcaccggcat cgacggccgg gtgtccaacg ccggggtgcc ccgctatgtc
     4921 gggctgggca gcgtgcacct ggtaccggtg tgcgccgagc cggtggaccc gcacaccatc
     4981 gccctggacg tgcgcaccac gcagtcagcc gtgcaggtct cgctcgccgc ccgggcgcgg
     5041 gtgctcgccg acgcggccga ggccgacgtc gcccgcgagc tggtccggga ggacggctac
     5101 gtcgcccagg acctgaccct cgacctggtg gagggtcagc cctggaccgt ggaaaaggtc
     5161 gccgcgctct acacctcgcg ggaccgggcg atcagcgagc cggccagcgc ggcccgccag
     5221 caagcgtcga acctgcccgg gttcgaagac ctgctcgccg cgcacgtgct ggcctgggac
     5281 caccggtggg agcggtgcga cctgaccatc caggcccgcg agccggaccg ggcccgggcc
     5341 tcgctcgccc tgcacgtgca gctgttccac gtgctgcaga ccgtctccga gcacgtcgtc
     5401 gacctcgacg tgggcatccc ggcccgcggg ctgcacggtg aggcctaccg cggccacatc
     5461 ttctgggacg agatgttcgt ctatcccttc ctgcaccagc gcatgcccga gctctcgcgc
     5521 tcgctgctgc tctaccggtg gcggcggctg ccgcaggcgc gccggctggc ccgcgaggcc
     5581 gggctgcgcg gcgccgcgtt cccctggcag agcggctccg acgggcgcga ggagacccag
     5641 gcggtgcacc tcaacccgcg ctccgggcgg tggctacccg accattcccg cctgcagcgg
     5701 cacatcaacg tcgccgtcgc cttcagcacc tggcagtact acgaggcgac cgcggacctc
     5761 gacttcctgg ccgagcacgg cgccgagctc atgctcgaga tcgcggcgat gtgggcgagc
     5821 ctggccacct acgaccgcac cgccgaccgc tatgacatcc gtggggtcat ggggccggac
     5881 gagttccacg acgcctaccc gtggcgcgac gagccgggca tcgacaacaa cgcctacacc
     5941 aacgtcatgg tggcctggtc gctggagcgc gccctgcgct gcgtgcgcat cctgccgacc
     6001 cgccggcggc gggagctgct ggacctgctc ggcgtgagca cgacagacct ggagcactgg
     6061 gaccacgtca gccgcaagct gcgtctgtgc tggcacggcg agggcatcct cagccagttc
     6121 gagggctatg agcggctcga ggagctggcg tgggccgact acgtcgagcg ctacggcgac
     6181 atcgcccgcc tcgaccggat cctcgaggcc gagggcgaca cgaccaaccg ctaccggctg
     6241 tccaagcagg ccgacgtact catgctcttc tacctgctct ctgccgacga cctggccgag
     6301 gtcctcgagc ggctcggcta ccgctacgac ccgatgatga tcccgcgcac catcgactac
     6361 tacctgcgac ggacgacgca cgggtcgacc ctgtccaagg tcgtgcacgc ctgggtcctg
     6421 gcccgcagca accggaccgg cgcctggcag tacctgctgg acgcgctgga gagcgacctg
     6481 gtcgacaccc agggcggtac gacgccggag ggcgtccaca tcggcgcgat ggccggctcg
     6541 atcgacctgc tgcagcgggc gttcaccggc ctggagaccc gcggtgagca gctctggctc
     6601 aacccggtgc tgcccgacca gctcaccggc ctgcagtttc ggctgcgcta ccgggagcac
     6661 tacggcgtgc acgtcgccgt cgaccacgac cgggtcgtgg tcggaggctc cggcgtcggc
     6721 cctcatcggc tgcatctgcg ggtgcgtgac gaggagctgc tgctcgaccc cggcggtgcc
     6781 cacgagttcg cgctgggcgc gcgagacccg ggctaggcca gagcttgtcc tagggtttcg
     6841 cggcatgcag ctgaccaagc tcggacacgc cgccctgctg ctcgagatcg ggcaggctcg
     6901 tctgctcatc gaccccggga cctactcgca cggtttcgaa gacctgcggg gcctcaccgg
     6961 gatcctcatc acccaccagc acgccgacca cctcgacccg gcccgcatcc acgcgctggt
     7021 ggccgccaac cccgatgccc tcgtgctcgc cgacgcggcg accacggcgg tgctcgcggg
     7081 cctcggcatc gccgccacgg cggccagcgc gggggacacc tttgagcccg gggtgctcgt
     7141 ggaggtggtc ggccaggagc acgccgtgat ccatccggac atcccggtgg tgcccaacgt
     7201 cggctatctg gtggccgagc gcctgctgca cccgggcgac gcgctgaccg tgcccgaccg
     7261 gccggtcgag ctcctcgcgc tgccgatgat ggccccctgg atgcgggtgg ccgacgccgt
     7321 cgactacgcc cgcgcggtgc acccggcggt cgtcgtaccg atccatgacg ccctgctggc
     7381 cgacccgcgc tcctaccaca agtggatcga ccagctcggc cccgccggca cccggctggc
     7441 cccggcggac ggcggtgagc ctgtctcgtt ctgacgtagg ctcgacccgg cgagccgctg
     7501 gtcggctcct tgcgagggag cggggcattc ggtggctggg ggaggagacg tgtcgagcac
     7561 ggaggtcgac ctgcgggtcg ccgaccgggt cctgaccgtg cccaacctgc tcagcctgat
     7621 gcggctgctc ggggtcccgg tcttcctctg gctgatcttc gccgagtacg acggtgcggc
     7681 gctcgtggtg ctcgcgtgtg ccggggccag cgactacctc gacggcctgc tcgcccggcg
     7741 gtggaaccag gtctcgcgcc ttggccagct gctcgacccg gcggccgacc ggctctacat
     7801 cttcgcgacg ctggtcgggc tgtcctaccg cgacatcgtg ccgatctggc tggccgtggt
     7861 cctcgtctcc cgtgacgcga tcctcacggc gtgcatcccg gtcctgcgcc ggcacggcta
     7921 tggcccgctg ccggtgcact tcctcggcaa ggccgccacg ttcaacctcc tctacgcgtt
     7981 cccgctcctg ctgctcggcg acggcagcac ggtcgtcgcg ctgctggcca agaccatcgg
     8041 ttgggccttc gcgatctggg gatcggcgct ctactggtgg gccggagtcc tctacctcgt
     8101 ccaggtcaa
//
LOCUS       DATIBB010000036         2126 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_1462501, whole genome shotgun sequence.
ACCESSION   DATIBB010000036 DATIBB010000000
VERSION     DATIBB010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2126
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1462501"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..521
                     /locus_tag="VLC50_01235"
     CDS             <1..521
                     /locus_tag="VLC50_01235"
                     /inference="COORDINATES: protein motif:HMM:NF016047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HSF26117.1"
                     /translation="YVATGTTFPDALAGAALAGSQGVPVLLTTRTSLPAATAQALARL
                     QPGRIVVLGDSGAVSDAVADALASYTSGSVTRLAGADRYATAAAVAAQYAPGVETAYV
                     ATGTTFPDALAGAALAGSQGMPVLLTAANAVPMPLSTAFLRLAPQQIVVLGDSGAVSD
                     PVASSLGVYGTG"
     gene            complement(543..1574)
                     /locus_tag="VLC50_01240"
     CDS             complement(543..1574)
                     /locus_tag="VLC50_01240"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HSF26118.1"
                     /translation="MSSTTDHATDWTTRLVRVDPGEIDLPLAAEVAVLWEVARLECEP
                     SMPPETAREAFAQLTYGWDGEPPELTIVRDGTALVAVGTLECPRRDNAHLGQFAAVVD
                     PGHRRRGLGSALLEAALARLRELGRRTVLAEAPEGSAGEGFLRAGGFASASTDVQRLL
                     PITQLDRDRLAEMLAAAQAASPGYRVEAVPIPVPDDRVEALLTVSRSINDAPIDELDL
                     EDEVYDADRLHGLEQSMAMRGRRLHWLLAWSPDGEPAGHTMVSVSAEHPQRGVQLDTS
                     VRREHRGHRLGLRLKLEMLERLRRLEPDLVSITTWNAASNAHMVGVNEAMGHRVERVW
                     HEYQRAVDA"
     gene            1801..>2126
                     /locus_tag="VLC50_01245"
     CDS             1801..>2126
                     /locus_tag="VLC50_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014142335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HSF26119.1"
                     /translation="MTTSPNRQQPSGMPVARYRAFPPIDLPDRTWPSRQITAAPRWLS
                     TDLRDGNQALIDPMSPARKRRMFDLLVGMGYKEIEVGFPSASQTDFDFVRQLVEGDAI
                     PDDVTIS"
BASE COUNT          271 a          786 c          770 g          299 t
ORIGIN      
        1 cttatgtggc aacgggtacg acgtttcccg acgcgttggc cggggcggcg ctggccgggt
       61 cgcagggtgt gccggtgctg ctgaccacgc gcacgagcct gccggcggcg acggcgcagg
      121 cgctggcgcg gttgcagccc ggacggatcg tggtgcttgg tgactcgggg gcggtctcgg
      181 acgcggtggc cgatgcgttg gcgtcgtaca cgagcgggtc ggtgacccgg ctggccggcg
      241 cggaccgtta tgcgacggcg gccgcggtgg ccgcgcagta cgccccgggc gtggaaacgg
      301 cttatgtggc aacgggtacg acgtttcccg acgcgttggc cggggcggcg ctggccgggt
      361 cgcagggcat gccggtgctg ctcaccgccg ccaacgcggt gcccatgccg ctgagcacgg
      421 cgttcctgcg gctggcgcca cagcagatcg tggtgctcgg cgacagcggg gccgtctcgg
      481 acccggtcgc gtcctcgctc ggcgtctacg gcaccggctg aacgcgctgc ccgagagacg
      541 gatcaggcgt cgacggcgcg ctggtactcg tgccagaccc gctcgacccg gtggcccatc
      601 gcctcgttga ccccgaccat gtgcgcgttg gacgcggcgt tccacgtggt gatgctgacc
      661 aggtcgggct cgagccggcg cagccgctcg agcatctcca gcttgagccg cagcccgagc
      721 cggtgcccgc ggtgctcgcg ccgcacgctg gtgtccagct gcacgccgcg ctgcgggtgc
      781 tccgcgctga ccgagaccat ggtgtgcccg gcgggctcgc cgtcgggaga ccaggcgagc
      841 agccagtgca gccgccggcc ccgcatggcc atgctctgct ctagcccgtg cagccggtcg
      901 gcgtcataga cctcgtcttc gaggtcgagc tcgtcgatcg gggcgtcgtt gatcgagcgc
      961 gacaccgtca gcagcgcctc gacgcggtcg tcgggcaccg ggatgggcac cgcctcgacg
     1021 cggtagccgg gcgaggcggc ctgggcagcg gccagcatct cggcgagccg gtcccggtcg
     1081 agctgggtga tcggcagcag ccgttgtacg tcggtgctcg cactcgcgaa tccgcccgcc
     1141 cgcaggaacc cctcgccggc cgagccctcg ggcgcctcgg ccagcacggt gcgccggccc
     1201 agctcgcgca ggcgagccag cgccgcctcg agcagcgcgg agccgagccc gcggcgccgg
     1261 tgaccggggt cgaccaccgc cgcgaactgg ccgaggtggg cgttgtcccg gcgcgggcac
     1321 tccagcgtgc ccaccgcgac cagcgcggtg ccgtcacgga cgatggtcag ctcgggcggc
     1381 tcgccgtccc agccataggt cagctgggcg aacgcctcac gcgccgtctc cggcggcatc
     1441 gacggctcgc actcgagccg ggcaacctcc cacagcaccg ccacctcggc ggcgagcggc
     1501 aggtcgatct cacccgggtc cacccggacg aggcgggtcg tccagtcggt ggcgtgatca
     1561 gtggtggagc tcatgccacc agacaagcgg atcgcgggag gcgggcgcga ctggatttcg
     1621 gtgccgaccc tcgcctacca tgtccgccaa tgcggttcgc gtccctgctc cttagctgcc
     1681 gcggcgaggc ctgacaggcc ggttcctcgc cgcggtgctg ctgcgttcgc cggcccacgc
     1741 ccgtcgcccg atggcccggc agccacccga gcagctatcc cagcagagga atcgcgtccc
     1801 atgaccacga gccccaaccg ccagcagccc tccggcatgc ccgtcgcgcg ctaccgggcg
     1861 ttccccccga tcgacctgcc cgaccgcacc tggccgtcgc gccagatcac cgcggccccg
     1921 cgctggctgt ccaccgacct gcgtgacggc aaccaggcgc tgatcgaccc gatgagcccg
     1981 gcccgcaagc ggcggatgtt cgacctgctg gtcggcatgg gctacaagga gatcgaggtc
     2041 ggcttcccgt cggccagcca gaccgacttc gacttcgtgc gccagctcgt cgagggtgac
     2101 gccatccccg acgacgtgac gatctc
//
LOCUS       DATIBB010000037         2827 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_147073, whole genome shotgun sequence.
ACCESSION   DATIBB010000037 DATIBB010000000
VERSION     DATIBB010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2827
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147073"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..606
                     /locus_tag="VLC50_01250"
     CDS             <1..606
                     /locus_tag="VLC50_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007071640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HSF26120.1"
                     /translation="STVPQGPYTIDRLGQEVLALLDDLGTARASYAGLSLGGMVGMWL
                     AAHAPARVNRLALLCTAALLGPASGWVDRAATVRTQGTRAIAEAVVARWFTPDWAGAH
                     PDVVRRHAAMLGTTPVEGYAGCCEAIASMDLRADLAAITAPTLVVAGADDLATPIAHA
                     EAILAGIDGARLAVVDSAAHLANVEQPDTVTALLLEHLDRD"
     gene            617..1015
                     /gene="pcaC"
                     /locus_tag="VLC50_01255"
     CDS             617..1015
                     /gene="pcaC"
                     /locus_tag="VLC50_01255"
                     /EC_number="4.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013733706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047575 - 4-carboxymuconolactone
                     decarboxylase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-carboxymuconolactone decarboxylase"
                     /protein_id="HSF26121.1"
                     /translation="MDDQQRHAQGMTVRRAVLGDAHVDRAIARTDEVTADFQDFITRY
                     AWGDVWSRPGLDRRMRSAITLAMLATLGHEGELGMHVRAALRNGLSPAEISEVLLQVG
                     LYAGVPAANRAFAVAQRVLAENSADEADAH"
     gene            1056..1853
                     /locus_tag="VLC50_01260"
     CDS             1056..1853
                     /locus_tag="VLC50_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013733707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HSF26122.1"
                     /translation="MTERAERSADFVQSLERGLAVIRAFDAEHSELTLSDVARRTGLT
                     RAAARRFLHTLVELGYMRFDGRLFALRPRVLALGYAYLSSQSLPEIAHPHMEELVAEV
                     HESCSVSVLDGDEVVYVARVATSRIMTVAISVGTRFPAYATSMGRVLLAAQPDDWLDG
                     YLAGTELAPLTSRTISDPSRLRAALARVRSQGFALVDQELEEGLRSLAVPIHDADGTV
                     IAAMNVSARASRGPSDVIRKQLLPPLLRTALAIEDDLAPAPAPTSRT"
     gene            1981..2763
                     /locus_tag="VLC50_01265"
     CDS             1981..2763
                     /locus_tag="VLC50_01265"
                     /inference="COORDINATES: protein motif:HMM:NF014534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HSF26123.1"
                     /translation="MSQEAFPLADVDVVVRGDISPEIVDYTRDKVVRTTRFADRPVLM
                     ARVVLTVDPDPAHDRPAQAEATIDVSGVPVRAKVAATTPTEAADFLQDRLRRRLDQLS
                     KRRSDRHRWVTEVLAAAQVGNQRPRYADRPAEEREVVRRKTFAVAPLTPDEAAYEMDL
                     LEHDFYLFVDANTSQDALIHRRPDGAFGLAEVGHALVPPPEPSRMVLEPAPPRLSEAD
                     AIARLDAAGEPFVFHVDPQTGRGRVLYRRFDGNYGVIVIAEH"
BASE COUNT          382 a         1058 c         1009 g          378 t
ORIGIN      
        1 tcaaccgtgc cgcagggccc ctacacgatc gaccggctcg gccaggaggt gctcgccctg
       61 ctcgacgacc tcggcacggc acgggcgtcg tacgccggac tgtcgctggg cgggatggtc
      121 ggcatgtggc tggccgcgca cgccccggcc cgcgtcaacc ggctggcgct gctgtgcacc
      181 gcggcgttgc tcggcccggc gtcgggatgg gtcgaccggg ccgccaccgt gcgcacccag
      241 gggacgcggg cgatcgcgga ggcggtggtg gcgcggtggt tcacgccgga ctgggcgggc
      301 gcccaccccg acgtcgtacg ccggcatgcc gcgatgctcg gcacgacacc ggtcgagggg
      361 tacgccggat gctgcgaggc gatcgcctcg atggacctgc gcgccgacct cgccgcgatc
      421 accgcgccga cgctggtggt ggccggtgcc gacgacctgg ccaccccgat cgcgcacgcc
      481 gaggcgatcc tcgccggcat cgacggggcc cggctggccg tcgtcgactc ggccgcccac
      541 ctggccaacg tggagcagcc ggacacggtc accgcgctgc tgctcgagca cctcgaccgt
      601 gactgaagga gacctcgtgg acgaccagca gcggcacgcc cagggcatga cggtgcgccg
      661 ggcggtgctc ggtgacgccc acgtcgaccg ggcgatcgcg cgcaccgacg aggtgaccgc
      721 cgacttccag gacttcatca cccgctacgc gtggggggac gtctggtcgc ggccgggcct
      781 cgaccggcgg atgcgcagcg ccatcacgct ggccatgctc gccaccctgg ggcacgaggg
      841 ggagctgggc atgcacgtgc gggccgcgct gcgcaacggg ttgagccccg ccgagatcag
      901 cgaggtgctg ctgcaggtgg gcctctacgc cggggtgccc gcggccaacc gggcgttcgc
      961 cgtggcccag cgtgtcctcg ccgagaactc tgccgatgag gccgacgccc actagcgtgc
     1021 gcacagcgga caaccggccg agtgcaagga gacccatgac cgagcgcgcc gagcgctccg
     1081 ccgacttcgt ccagtcgctc gagcgcgggc tggccgtgat ccgggccttc gatgcggagc
     1141 actccgagct gacgctctcc gacgtggccc gccggaccgg gctgacccgg gccgcggccc
     1201 gacggttcct gcacacgctg gtcgagctgg gctacatgcg cttcgacggt cggctgttcg
     1261 cgctgcgccc acgggtgctg gcgctcggct acgcctacct gtccagccag agtctgcccg
     1321 agatcgccca cccgcacatg gaggagctgg tcgccgaggt ccacgagtcg tgctcggtgt
     1381 cggtgctcga cggcgacgag gtcgtctacg tcgcccgggt ggccaccagc cggatcatga
     1441 cggtggcgat cagcgtcggc acccggttcc cggcctacgc gacgtcgatg ggccgagtgc
     1501 tgctggccgc ccagcccgac gactggctcg acggctacct ggccggcacc gagctggcgc
     1561 cgttgaccag ccgcacgatc tccgacccgt cgcggctgcg cgccgcgctg gcacgggtgc
     1621 gcagccaggg cttcgcgctc gtcgaccagg agctcgagga ggggctgcgc tcgctcgccg
     1681 taccgatcca tgacgccgac ggcacggtga tcgcggcgat gaacgtgtcg gcccgggcca
     1741 gccgcggacc ctccgacgtc atccgcaagc agctgctgcc cccgctgctg cgcacggccc
     1801 tggcgatcga ggacgacctc gccccggccc cggcacccac ctcccgcacg taatgcgacg
     1861 caggccactc tcgtggtgat ccataccaag aagtgacgta cgtcccttga gcgcccggcg
     1921 tacggtcgga ttggctcgag ccaacgggtc gctcgagcac cccgccgagg aggttggctc
     1981 atgtcgcagg aggctttccc gctcgccgat gtcgacgtcg tggtccgtgg tgacatctcg
     2041 ccggagatcg tcgactacac ccgcgacaag gtggtccgaa ccacccggtt cgccgaccgg
     2101 ccggtgctga tggcccgggt ggtgctcacg gtcgaccccg acccggccca cgaccggccc
     2161 gcccaggctg aggcgaccat cgacgtctcc ggggtcccgg tgcgggccaa ggtcgccgcg
     2221 accaccccga ccgaggccgc cgacttcctg caggaccggc tgcgccgccg cctcgaccag
     2281 ctgagcaaac ggcgctccga ccggcatcgc tgggtcaccg aggtgctggc cgcggcccag
     2341 gtcggcaacc agagacctcg gtacgccgac cggccggccg aggagcgcga ggtggtgcgg
     2401 cgcaagacgt tcgcggtcgc gccgctcact cccgacgagg cggcctacga gatggacctg
     2461 ctcgagcacg acttctacct gttcgtcgac gccaacacca gccaggacgc gttgatccat
     2521 cgccggcctg acggggcatt cgggctcgcc gaggtcggcc acgcgctggt gccgccgccg
     2581 gagccgagcc ggatggtcct cgagccggcg ccgccccggc tgtccgaggc cgacgcgatc
     2641 gcccgcctcg acgccgccgg cgaaccgttc gtgttccacg tcgacccgca gaccgggcgc
     2701 ggccgggtgc tctaccgacg cttcgacggc aactacggcg tgatcgtcat cgcggagcac
     2761 tgagtcggcc gctcgacccg acggcccgcg gcgccttatg gcatcgcggg ccgtcggtgt
     2821 gacgccg
//
LOCUS       DATIBB010000038         6096 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_15884, whole genome shotgun sequence.
ACCESSION   DATIBB010000038 DATIBB010000000
VERSION     DATIBB010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 6096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6096
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15884"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(4..1059)
                     /locus_tag="VLC50_01270"
     CDS             complement(4..1059)
                     /locus_tag="VLC50_01270"
                     /inference="COORDINATES: protein motif:HMM:NF021599.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2332 domain-containing protein"
                     /protein_id="HSF26124.1"
                     /translation="MADRERLAQKVAHQGEICARLGSALYGELLARAAEDIRAGGPVA
                     RVLAGYEDAPGPAATALRLMGGVHRVVLTGGAPELAAYYPSVGGRADGVAAWPVFRAV
                     VEEHHDEIRRMLVGPPQTNDVGRSAALVGGLLHLTAATGRPVRLFELGTSAGLNLRAD
                     HYRITGAGTAWGPADSPVVIDGWQGLAPPTDAPLEVVERVGCDVAPIDVGTEEGRLLL
                     SSYVWADHVDRMTRLRGAFEVAARVPAQIVHSAAVDCMSKLTLQPGALTVLWHSVMWQ
                     YVDPDEQRAVLKQINEVAASATEQAPFAHLFLEPRRREPGGPHVFLVTASLWPHWPDE
                     RVLASSRAHGVPTTWEL"
     gene            complement(1052..1543)
                     /locus_tag="VLC50_01275"
     CDS             complement(1052..1543)
                     /locus_tag="VLC50_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017986924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HSF26125.1"
                     /translation="MSMFGNQSQGIPTPPTGLPVGSYGTYAEAQRAVDYLADERFPVE
                     NVTIVGSDLQMVERVLGRLSWGRAAGAGAATGAWFGLFVGILLSIFASESTSFLVLAL
                     SGLLYGAVFGIIFGLISYALTGGRRDFTSRSQIVAGRYDVLCQHQKAEQARELLAKLS
                     LRG"
     gene            complement(1650..2855)
                     /locus_tag="VLC50_01280"
     CDS             complement(1650..2855)
                     /locus_tag="VLC50_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014138717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HSF26126.1"
                     /translation="MGRLAQTEGLSDVQQEILRTVRDFVDKEILPVATELEHRDEYPQ
                     AIVDGMKELGLFGLTIPEEYGGIGESLLTYALAVEEIARGWMSVSGIINTHFIVAYML
                     AKHGTEEQKQHYLPLMATGGVRGAFSMSEPGCGSDVSAIKTKAVRIGDAGDRYRIDGQ
                     KMWLTNGGSANLVAVLVKTDEGAESVYRNMTTFLVEKEPGFGETAPGLTVPGKIEKMG
                     YKGVDTTELVFTGHRIGAGQVLGGTPGRGFYQMMDGVEVGRVNVAARGAGVATRAFEL
                     AVAYAQQRETFGKKIAEHQAVAFRLADMAVKVEAAHQMMVRAARMKDSGARNDLEAGM
                     AKYLASEYCAQVVEDSFRIHGGYGFSKEYEIERLYREAPMLLIGEGTADIQRMIIARR
                     LLEDYKLPD"
     gene            complement(2942..3739)
                     /locus_tag="VLC50_01285"
     CDS             complement(2942..3739)
                     /locus_tag="VLC50_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012180903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HSF26127.1"
                     /translation="MTEVGSLVALVTGAGSPGGIGFACARALVGTGASVAIVSTTDRI
                     HERAAELAVEAAPGERGTPSVVGVVADLMDPDAVRRMVAEVVAQLGQVDVLVNNAGMV
                     AVGQPETSAPVGELSIDAWRGGIARNLDTAFLVTSAVLPGMLRRGWGRIVNVASTSGY
                     VNAMPEQAAYTAAKAAMVGLTKAVAVEAAGHAVTCNAVAPGWIETLSSTPAEIGHGLR
                     TPMGRCGRPDEVAAAVAFLASPAASYITGQCLVVDGGNSVLEAYDAV"
     gene            complement(3764..4747)
                     /locus_tag="VLC50_01290"
     CDS             complement(3764..4747)
                     /locus_tag="VLC50_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013673237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HSF26128.1"
                     /translation="MALAPARRSDRTLRPRRSCLAVPGSSTKMLGKAKGLGADQVFLD
                     IEDSVAPLAKAQARANIIAALTEGGWGQTVRTVRVNDWTTQWTYRDVVDVVEGAGAHL
                     DAIMLPKVQNAEQVVALDLLLTQIETAMGLEVGRIGIEAQIENARGLVEVDAIAEASP
                     RLETIIFGPADFMASINMKSLVVGEQPPGYDVGDAYHYILMRLLMAARANDLQAIDGP
                     YLQIKDVDGFRRVAARSAALGFDGKWVLHPGQLEACNEAYSPRQDDYDHAELILDAYA
                     WHTSEAGGARGAVMLGDEMIDEASRKMAQVVAAKGRAAGMARTSTFAPPTS"
     gene            complement(4868..5338)
                     /locus_tag="VLC50_01295"
     CDS             complement(4868..5338)
                     /locus_tag="VLC50_01295"
                     /inference="COORDINATES: protein motif:HMM:NF015962.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0050532 - 2-phosphosulfolactate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HSF26129.1"
                     /translation="MGPLPPRLVVPSPNGATIATLAVTSGVVVAACLRNARAVGAWLG
                     QRYGGTEAPVTVIAAGERWPDGSLRPAFEDWFGAGAVITSFAEHGAVSLSPDAEAAGA
                     TYDGMREGVLEALRRCDSGVELAQAGFGADVELAAAVDVSDAVPVLAAGAFRAS"
     gene            complement(5616..>6096)
                     /locus_tag="VLC50_01300"
     CDS             complement(5616..>6096)
                     /locus_tag="VLC50_01300"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HSF26130.1"
                     /translation="VDVSLSPRALFGDMLALAGGVFSGAYVVVGAQVRRHASTSAYTA
                     VCYGVCAGLLLVVCLATGQALTGYAAIDWARLVALAVAAQLLGHSVFNYVLRRVSPTV
                     VSLTILLEVPGAALIAALFLGQIPPLAAIPAVGLILAGIGIVIGARPGPQPTAMPGE"
BASE COUNT          898 a         2259 c         2104 g          835 t
ORIGIN      
        1 gggctagagc tcccaggtgg tcggcacgcc gtgggcgcgc gaggaggcga gcacccgctc
       61 gtccggccag tgcggccaca gcgaggcggt gacgaggaag acgtgcggcc cgcccggctc
      121 ccgccggcgc ggctcgagga acaggtgggc gaacggcgcc tgctcggtcg ccgacgcggc
      181 gacctcgttg atctgcttga gtacggcgcg ctgctcgtcg gggtcgacgt actgccacat
      241 caccgagtgc cagagcaccg tcagcgcgcc cggctggagc gtgagcttgg acatgcagtc
      301 gaccgcggcc gagtgcacga tctgggccgg cacccgggcc gccacctcga acgccccgcg
      361 cagccgggtc atccggtcga catggtcggc ccagacgtag gacgagagca gcagccgccc
      421 ctcctcggtg ccgacgtcga tcggcgcgac gtcgcagccg acccgctcga cgacctcgag
      481 cggggcgtcg gtcggcggcg ccaggccctg ccagccatcg atgaccaccg gggagtcggc
      541 cgggccccag gcggtgccgg ccccggtgat ccggtagtgg tcggcccgca ggttgagccc
      601 ggcgctggtg ccgagctcga agagccgcac gggacggccg gtcgcggcgg tcaggtggag
      661 caaccccccg accagcgcgg ccgaacggcc cacgtcgttg gtctgcggcg gcccgacgag
      721 catccggcgg atctcgtcgt ggtgctcctc gacgaccgcg cggaacacgg gccacgcggc
      781 gaccccgtcg gcccggccac cgacgctcgg gtagtaggcg gcgagctcgg gggccccacc
      841 ggtgagtacg acgcggtgga cgccgcccat cagccgcagc gcggtcgccg ccggtcccgg
      901 cgcgtcctcg tagccggcca ggacccgggc gaccgggcca cccgcacgga tgtcctcggc
      961 cgcccgcgcc aacagctcgc cgtagagcgc cgatcccaac cgggcgcaga tctcgccctg
     1021 gtgggcgacc ttctgcgcaa gtcgctcgcg atcagccacg gagcgacaac ttggccagca
     1081 gctcccgagc ctgctcggcc ttctggtgct gacacagcac gtcatagcgc ccggcgacga
     1141 tctgcgagcg cgacgtgaag tcgcgccgac cgccggtcag ggcgtaggag ataaggccga
     1201 atatgatgcc aaacacggca ccgtagagta gaccagacag cgcgagaacc agaaaactgg
     1261 tgctctcgct ggcaaagatc gacagcagga tgccgacgaa caggccgaac cacgcgccgg
     1321 tggccgcccc ggcgccggcc gcgcggcccc acgagagccg gcccagcacc cgctcgacca
     1381 tctgcaggtc gctgccgacg atcgtgacgt tctcgaccgg gaacctctcg tcggccaggt
     1441 agtcgaccgc gcgctgcgcc tcggcatagg tcccgtagga cccgaccggc agcccggtcg
     1501 gtggggtggg gatgccctgg ctctggttgc cgaacatgct catggccgct cctcacgtcg
     1561 tctgggcctg tcgtgcccac tacgacgacc ttgcctgcct ccggcgaggg cggccagccg
     1621 ctcagaccac gtcgtacgac gaaagtggct cagtcgggca gcttgtagtc ctcgagcagc
     1681 cggcgggcga tgatcatgcg ctggatgtcg gcggtgccct caccgatgag cagcatcggc
     1741 gcctcgcggt agagccgctc gatctcgtac tccttggaga agccgtagcc gccgtggatg
     1801 cggaacgagt cctcgaccac ctgggcgcag tactcgctgg cgaggtactt ggccatgccg
     1861 gcctcgaggt cgttgcgggc gccggagtcc ttcatccgcg cggcccgcac catcatctgg
     1921 tgggcggcct cgaccttgac cgccatgtcg gccagccgga acgccaccgc ctggtgctcg
     1981 gcgatcttct tgccgaacgt ctcgcgctgc tgggcatagg ccacggcgag ctcgaacgcg
     2041 cgggtggcca cgccggcgcc gcgggcggcc acgttgaccc ggcccacctc gacgccgtcc
     2101 atcatctggt agaagccgcg cccgggggtg ccgccgagca cctgcccggc gccgatccgg
     2161 tggccggtga agacgagctc ggtggtgtcg acccccttgt agcccatctt ctcgatcttg
     2221 ccgggcacgg tcagcccggg cgcggtctca ccgaacccgg gctccttctc gaccaggaac
     2281 gtcgtcatgt tgcggtagac cgactcggca ccctcgtcgg tcttcacgag tacggcgacc
     2341 aggttggcgc tgccgccgtt ggtcagccac atcttctggc cgtcgatccg gtaccggtcg
     2401 ccggcgtcgc cgatccggac cgccttggtc ttgattgcgg acacgtcgga gccgcagccg
     2461 ggctcggaca tcgagaacgc cccgcgcacc ccgccggtgg ccatcagcgg caggtagtgc
     2521 tgcttctgct cctcggtgcc gtgcttggcc agcatgtagg cgacgatgaa gtgcgtgttg
     2581 atgatgccgc tcacgctcat ccagccgcgg gcgatctcct cgacggccaa cgcataggtc
     2641 agcagcgact cgccgatccc gccgtactcc tccgggatcg tcagcccgaa caggccgagc
     2701 tccttcatcc cgtcgacgat cgcctgcggg tactcgtcgc ggtgctcgag ctcggtggcg
     2761 accggcagga tctccttgtc gacgaagtcg cgcaccgtgc gcaggatctc ctgctgcacg
     2821 tcggacagtc cctcggtctg cgccagtcgg cccatgctgc gcactccttg ctcggcttac
     2881 gcggagtatc tccggtcacg acgccgccga cagcatgacg cgtctcacag ggcggcgatc
     2941 atcacacggc gtcgtacgcc tcgagcacgc tgttgccgcc gtcgaccacg aggcactgtc
     3001 ccgtgatgta ggacgcggcc ggcgatgcga ggaatgcgac cgcagcggcc acctcatcgg
     3061 gccggccgca gcggcccatg ggtgtgcgca gaccgtgccc gatctcggcc ggagtggacg
     3121 acagcgtctc gatccagccc ggggccaccg cgttgcaggt caccgcatgc ccggcggcct
     3181 cgacggcgac cgccttggtg aggcccacca tcgctgcctt cgccgccgtg taggccgcct
     3241 gttcgggcat cgcgttgacg tagccagacg tcgaggcgac gttgacgatc ctcccccaac
     3301 cgcgtcggag catcccgggc agcaccgcac tggtcaccag gaacgcggtg tccaggttgc
     3361 gcgcgatgcc gccgcgccac gcgtcgatgg acagctcgcc gaccggcgcc gacgtctcgg
     3421 gctggccaac cgcgaccatg ccggcgttgt tgaccagcac gtcgacctgg ccgagctgcg
     3481 cgacgacctc ggccaccatg cgccgtacgg catcgggatc catgaggtcg gcgaccaccc
     3541 ccacgacgct gggcgtgccg cgctcgcccg gtgccgcctc gaccgctagc tcggccgcac
     3601 gctcgtggat gcggtcggtg gtggacacga tcgccacgct cgcgccggtg cccaccaggg
     3661 cacgagcgca cgcgaagccg atcccgcccg ggctgcccgc ccccgtgact agcgcgacca
     3721 ggctgccgac ctcggtcacc ccgcccacgc tagccacgcc ggatcagctc gtgggcggcg
     3781 cgaacgtcga ggtgcgcgcc atgccggcgg cccgaccctt ggccgcgacc acctgcgcca
     3841 tcttgcgcga ggcctcgtcg atcatctcgt cgccgagcat caccgcaccg cgcgccccgc
     3901 cggcctcgga ggtgtgccag gcgtaggcgt cgaggatcag ctcggcgtgg tcgtagtcgt
     3961 cctgtcgtgg gctgtaggcc tcgttgcacg cctcgagctg gcccgggtgc agcacccatt
     4021 tgccgtcgaa gccgagcgcc gcgctgcggg cagccacccg gcggaacccg tcgacgtcct
     4081 tgatctgcag gtaaggcccg tcgatcgcct gcaggtcgtt ggcccgggcg gccatcagca
     4141 ggcgcatgag gatgtagtgg taggcgtcgc cgacgtcata gccgggcggc tgctcgccga
     4201 ccacgagcga cttcatgttg atgctggcca tgaagtcggc cgggccgaag atgatggtct
     4261 ccagccgtgg actggcctcg gcgatcgcgt cgacctccac gagcccgcgg gcgttctcga
     4321 tctgcgcctc gatgccgatc cggccgacct ccaggcccat cgcggtctcg atctgggtga
     4381 gcagcaggtc cagcgcgacc acctgctcgg cgttctgcac cttgggcagc atgatcgcgt
     4441 cgaggtgcgc gccggcgcct tcaaccacgt ccaccacgtc gcggtaggtc cactgcgtgg
     4501 tccagtcgtt gacccgcacg gtgcgcacgg tctggcccca gccgccctcg gtcagcgcgg
     4561 cgatgatatt ggcccgggcc tgcgccttgg ccagcggcgc gaccgagtcc tcgatgtcga
     4621 ggaacacctg gtcggcgccc agccccttgg ccttgcccag catcttggtg ctgctgccgg
     4681 gcaccgccag gcaggaacgg cgcgggcgca gcgtccggtc ggagcgacga gcgggggcga
     4741 gagccatgcg gtcacctttc gcgccgagcg gcgcgcggcc gcttcacgac gtacgacggg
     4801 attggccgcg actctagcgt cgcgcctccg gttcacctac ccgccgctag ccgtgatctg
     4861 caacacctca gctcgcccgg aacgcaccgg cggcgagcac gggcacggca tcgctgacgt
     4921 cgactgccgc agcgagctcg acgtcggcgc cgaaccccgc ctgcgccagc tcgacacccg
     4981 agtcgcaccg acggagcgcc tcgagcacgc cttcgcgcat cccgtcgtac gtcgcacctg
     5041 ccgcctccgc gtcgggcgag agcgagacag cgccatgttc tgcgaacgag gtgatgacgg
     5101 caccggcgcc aaaccagtct tcgaacgcgg ggcgcagcga cccgtccggc cagcgctcgc
     5161 ccgccgcgat cacggtgacg ggcgcctccg tgccgccgta ccgctgaccc agccaggcac
     5221 ctaccgcgcg ggcattgcgc aggcacgccg ccacgacgac accggaagtg acggcgagtg
     5281 tcgcgatcgt ggcgccgttg ggcgagggaa ccaccagccg gggcgggaga gggcccacgg
     5341 tcaagcttgc gggcgacagc gaccacgggt gctccgccgt gacctcccgc cgcctctcga
     5401 ccaggaccgc atcgacgtcg tgggccaacc gctgcgccga ggcgtcgtgc cgcttggcgg
     5461 gtagacggca gcgccgcggt ccacggcgac cgtcaccgcg gtcgtgaacg acaggacgtc
     5521 cacgatcacg agcactccgt ggggcgacgc gagacgctcc gccgccaccg ggccccaccc
     5581 gcagcgcacc agaggcctgc ccaccaccga gaagctcact cgcccggcat cgcggtgggc
     5641 tgcggaccgg gccgggcgcc gatgacgatg ccgatcccgg cgaggatcag gccgaccgcc
     5701 gggatggccg ccagcggggg gatctgcccg aggaacagcg ccgcgatcag cgccgcgccg
     5761 ggcacctcga gcaggatggt cagcgacacc acggtcgggc tgacccggcg cagcacgtag
     5821 ttgaagaccg agtggcccag cagctgcgcc gcgacggcga gtgcgaccag ccgggcccag
     5881 tcgatggccg cgtagccggt cagcgcctgc ccggtcgcca ggcagaccac gagcagcagg
     5941 ccggcgcaca cgccgtagca gactgccgtg taggccgacg tgctcgcgtg ccggcgcacc
     6001 tgggccccga ccacgacgta ggcgccggag aagacgccgc cggccagcgc gagcatgtcc
     6061 ccgaacaacg cccgcggcga cagcgacacg tcgacg
//
LOCUS       DATIBB010000039        11080 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_1068049, whole genome shotgun sequence.
ACCESSION   DATIBB010000039 DATIBB010000000
VERSION     DATIBB010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 11080)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11080)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11080
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1068049"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(20..1846)
                     /locus_tag="VLC50_01305"
     CDS             complement(20..1846)
                     /locus_tag="VLC50_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009300762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HSF26131.1"
                     /translation="MTSPSPATAHTAAARVDGYAPLRAYAAIGAGRTVALVADDGSID
                     WLALPDLDSPSVFAALLDAERGGRATLAPTVAHTVSRRYLPETNVLETTFVTDTGTVA
                     VLDAMTVRGHALGPLRELQRRVVGVRGRVPMTWRVVPRFGYGTRRTRLDWRAGVPVAS
                     AGSDAIAVRTFGAGTVEVDAEAVTGAFETDPGSEAVIALCMAHQEPLVLPSRAELDAR
                     FDETVRTWRAWAGARGYDGPWPDAVSRSALALKLLVHAPSGAIAAAATTSLPEELGGE
                     RNWDYRFCWVRDAAFTLDALFRLGWAPEAQAYFWWLVHASALTHPRLQPLYRLDGGPQ
                     ARERVLPLAGYRGSAPIRVGNAAADQLQLDTYGELLQTAWLYAQSGQPLEPDIGRRLA
                     ETADLVCRLWSQPDAGIWEVRSAPRHFTQSKMMCWVALDRATRLAEAGLIPGRHAQRW
                     RTQAAAIRDFVETRCWSPTKRSYRRDADSDELDAAVLLGVLADYGDPHSPRWVDTVDA
                     IRRELGHGPYLYRYTGEDGLAGGEGAFLTCSFWLAEALARTDRVAEATALMNELVALS
                     NDVGLYAEEVDPSTGEFLGNLPQALSHLALISAATTIAAVRR"
     gene            complement(1911..2849)
                     /locus_tag="VLC50_01310"
     CDS             complement(1911..2849)
                     /locus_tag="VLC50_01310"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HSF26132.1"
                     /translation="MSIRAARLAAHGQPLLVQDVELPTPSADEVLVELAYGGVNPFDG
                     YVAAGRVAPDAPLPRTLGGEASGLLDGRPVVIAGEGLGTTRDGVFAEAAVVPRDAVVE
                     VPAGVSLRDAAAMGIVGLTAYRVVQTAHIGPDDRVLVLGGSGAVGLSAISYAASTGAR
                     VWGQTRSAAKATAIRDMGAKDAVVADAPGLAAAVRDINPTVVLDPLGGAFTSAVLTVM
                     AARGRLVLFGASAGSEATLDLLPLYRNQIQLLTYAGVQATRDERRAGISAALTAAAEG
                     RLRVGIGREVPLEAANDALTALADASIAGKVLLRLR"
     gene            complement(2872..3357)
                     /locus_tag="VLC50_01315"
     CDS             complement(2872..3357)
                     /locus_tag="VLC50_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006552932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HSF26133.1"
                     /translation="MDTGGLVLVGLVILLGLLGVVVPLLPGLPIVLGAIAVWAIVVGT
                     PTAWTVLAGAAALIVAGSLVKYVVPARRLRAGGVPWASTAAATVLAIVGFFVIPVIGA
                     VIGFVAGMYVAERLRLGSHELAWPSARAAVMAVGVSLLIELGAGLLAAGLWLAAVIMT
                     A"
     gene            complement(3466..3696)
                     /locus_tag="VLC50_01320"
     CDS             complement(3466..3696)
                     /locus_tag="VLC50_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003981845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF26134.1"
                     /translation="MTAALAPVHELTAADRCDRCGAQAYVRVILASGGELLFCAHHAR
                     RYEPTLKTMAAEIHDESDRLASTPAIAPADER"
     gene            3944..6070
                     /locus_tag="VLC50_01325"
     CDS             3944..6070
                     /locus_tag="VLC50_01325"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013838941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HSF26135.1"
                     /translation="MSAQPSEPLPKVDGYTARHLSVLDGLEAVRKRPGMYIGSTDSRG
                     LMHCLWEIIDNAVDEALGGHCQRIEVALHADGSVEVRDDGRGVPIDIEPKTGLPGVQV
                     VFTKLHAGGKFGGGSYTASGGLHGVGASVVNALAARLDVEVDRGGKVHAMSFRRGEPG
                     VYDDGRPGPSPESPFAPFTARSELRVVGKVKRGVTGTRVRYWPDRQIFLPDATFSFDE
                     LVTRARQTSYLVPGLTLVVRDERPRPDADGSVEETFHHEEGIGEFAEFLAPDPAVTDV
                     WRLRGTGHFKETVPVLDERGHMRPAEVERECVVDVALRWGTGYDTRLCSFVNIIATPK
                     GGTHVAGFEHALLKTFKKAIEANSRRLKTGKDDKLDKDDVLEGLTTVVTVRLAEPQFE
                     GQTKEVLGTAAVRGIVARVVERELTARLTSTKRGDKAQAATVLDKVVAAMKTRVAARI
                     HKETQRRKNALETSSLPAKLADCRSDDVARTELFIVEGDSALGTAKFARSSEFQALLP
                     IRGKILNVQKASPGDMFKNAECAAIIQVVGAGSGRTFDLEAARYGKVVFMSDADVDGA
                     HIRTLLLTLFFRYMRPMVEAGRVFAAVPPLHRVEVINPGGKKNDVVYTYSDAELRAVL
                     ADLARKGKRYKEPIQRYKGLGEMDADQLAETTMDPRHRTLRRVTMTDAEAAERVFELL
                     MGNDVAPRREFIVAGADELDRARVDA"
     gene            complement(6112..6600)
                     /locus_tag="VLC50_01330"
     CDS             complement(6112..6600)
                     /locus_tag="VLC50_01330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF26136.1"
                     /translation="MAPMTDGVEARYPDPMAGYRTLARLLGVGLLAGAVIGGAVGTIV
                     VGLQAYALFGLLAGATVGMVAGVVTQGLTFTVLLVARRAAPALSRRAAYVVIAAIPVA
                     TAGGLARWAASAPSPGGSDGSDGMTIVVTAVAVMLAVAAVMLAASWCLAPLLGRQHAP
                     RP"
     gene            6805..7623
                     /locus_tag="VLC50_01335"
     CDS             6805..7623
                     /locus_tag="VLC50_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020510462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase N-terminal
                     domain-containing protein"
                     /protein_id="HSF26137.1"
                     /translation="MSSWNGMTYEGKDTLLRVVREEARSFFALVSDPQAWEAPTQSTT
                     WQVRDVVGHLVDTTESYFPAFDAARAHAEVEPAYGLPGMAARVDERAVALRSVPREEL
                     LERLRGDFAKMMEILGALGPDEWGGLLVPHFYMGPLPAFFYPAFQLMDYGVHSWDLRQ
                     STGRAHGFSGEAADLLVPFMFVLWQATATCGPDTEPTTLGIRVTSGPNAGATRVSVGP
                     EGISFEPGEITDLATVLEFDPGSFVLTAFGRSNAGTIRGDRVTADRYLNLFFRI"
     gene            7661..8158
                     /locus_tag="VLC50_01340"
     CDS             7661..8158
                     /locus_tag="VLC50_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020420769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HSF26138.1"
                     /translation="MADIDLSAIEAERARIRETYLRPTGSRPASSAQGLHHFAVVCSD
                     VERTVNFYQGLLEFPLTEIFENRDYKGSNHFFFDVGHGNLLAFFDFPGLDVGPYAEVL
                     GGLHHIAISVERPTWERLRGKLDAAGVDYLEESGSSLYFRDPDGTRLELLADPLGQMY
                     GLTVL"
     gene            complement(8160..9086)
                     /locus_tag="VLC50_01345"
     CDS             complement(8160..9086)
                     /locus_tag="VLC50_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020139178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrase ferredoxin"
                     /protein_id="HSF26139.1"
                     /translation="MELGPRCSLVSADVGEPLPGTAPVAAAWLLVEQPGPWGREAPTQ
                     SHLPGEVAHPLAAAAAAAGVRLGLVRRPGRHPDRGAPRSVFLAFTRPGASALRLLRMR
                     DPRELLDLDLTRLATQGPGSVGEPSPPVLAVCTNARRDRCCAIGGRPLAAHAAAAFGG
                     ADAGRVWETSHLGGHRFSPTAVVLPQGSVHGRLTDPQAVDLLVAADAGRLAAHGYRGR
                     SAFSKPAQVGEALVRAKLGIEGVDDLDAASVWDAGGDGGPGWLVEVTHRDGRRWTVAV
                     HEVESAQARPESCGKPAVALRSLLPSRLQQVT"
     gene            complement(9077..>11080)
                     /locus_tag="VLC50_01350"
     CDS             complement(9077..>11080)
                     /locus_tag="VLC50_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017933774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HSF26140.1"
                     /translation="VDMRLNYDGTRDEPLILPAQYPNLLVNGSSGIAVGMATNMAPHN
                     LIEVIAAARHLIKRPEATLDELVRFVPGPDLPTGGTIVGLDGIREAYETGRGSFRIRA
                     TARIESVTARKKGIVVTELPYTVGPEKVIEKIKDLVQAKKLLGISDVTDLTDRDKGLR
                     LVIEVKNGFNPEAVLAKLYKLTPMEDSFGINNVALVDGQPRTLGLRELLTVYVDHRLD
                     VVRRRSLFRRRKAADRLHKVEGLLIAILDIDEVIAVIRSSDDAGAARERLRSVFDLSE
                     VQADYILEMQLRRLTKFSRIELEKEKSALEADIEALDAILGDDARLREVVSDELAAVA
                     KTYGTPRRTVLLEAAGAPATAVVPLEVSDDPCWVLLSSTGLVARTSDDAPMPVSTGRA
                     KHDVIVSAVRATARGEVAVLTSAGRMRRLSVLELPTRPPTASAPNLSGGLPLGALLAL
                     DADESVLTLVSLSTDSPGVVLGTAAGVVKRVAPDYPANKHEWEVIGLKPGDRVIGAAE
                     LRTGDEDLVFVTSDAHLLRFSAAGVRPQGRGAGGVAGVRCSAGVQVVFFGAVDPGADA
                     VVVTVSGSSAALPGTDAGSVKVAPFTEFPAKGRGTGGVRCHRFLKGEDALVLAWAGPG
                     PARAAAASGVAVELPAATGRRDGSGTPAAMPIAGVAGPAAAWS"
BASE COUNT         1601 a         4032 c         3997 g         1450 t
ORIGIN      
        1 gccagggcgg cccagacgct catcggcgca ccgccgcgat ggtcgtcgcg gcactgatca
       61 gcgccaggtg gctgagcgcc tgcggcaggt tgcccaggaa ctcgccggtg ctggggtcga
      121 cctcctcggc gtagagcccg acgtcgttgc tcagggccac cagctcgttc atcagtgcgg
      181 tggcctccgc gacccgatcg gtgcgcgcca gggcctcggc gagccagaac gagcaggtga
      241 ggaaggcgcc ctcgccgccc gccagcccgt cctcgccggt gtagcggtag aggtagggcc
      301 cgtgcccgag ctcgcggcgg atcgcgtcga cggtgtcgac ccaccggggc gagtgcgggt
      361 cgccgtagtc ggccaggacc ccgagcagta cggcggcgtc cagctcgtcg gagtcggcgt
      421 ctcggcggta gctgcgcttg gtcggggacc agcagcgggt ctcgacgaag tcacggatcg
      481 ccgcggcctg ggtgcgccat cgctgggcgt gccggccggg gatgaggccc gcctcggcca
      541 gccgggtcgc acggtccagc gccacccagc acatcatctt ggactgggtg aagtgccggg
      601 gcgcgctgcg cacctcccag atgccggcgt cgggctggga ccagagccga cagaccaggt
      661 cggccgtctc ggcgaggcga cggccgatgt cgggctcgag cggctggccg gactgggcgt
      721 agagccaggc ggtctgcaac agctcgccgt aggtgtcgag ctgcagctgg tcggctgccg
      781 cgttgccgac cctgatcggc gccgacccgc gatagccggc cagcggcagg acgcgttcac
      841 gggcctgcgg cccgccgtcg aggcggtaga gcggttgcag ccgcggatgg gtcagtgccg
      901 aggcgtggac cagccaccag aagtaggcct gggcctccgg ggcccagccc aggcggaaca
      961 gcgcgtccaa cgtgaacgcc gcgtcgcgga cccagcagaa gcggtagtcc cagttgcgct
     1021 cgccgccgag ctcctcgggc agggacgtcg tcgcggccgc cgcgatggcc ccggaggggg
     1081 catggaccag cagcttgaga gccagcgcgc tgcggctgac cgcgtccggc cagggaccgt
     1141 cgtagccgcg ggcgccggcc caggcccgcc acgtgcgcac cgtctcgtcg aaccgggcgt
     1201 ccagctcggc ccgcgacggc agcaccagcg gctcctggtg cgccatgcac agggcgatca
     1261 ccgcctccga gccggggtcg gtctcgaacg cgccggtgac cgcctcggcg tccacctcga
     1321 cggtcccggc cccaaacgtg cgtacggcga tcgcgtcgct gcccgccgag gcgacgggca
     1381 ccccggcccg ccagtccagt cgggtccgtc gcgtgccgta gccgaaccgg gggacgaccc
     1441 gccaggtcat cggcacccgc ccgcgcacgc ccaccacgcg ccgctgcagc tcgcgcaacg
     1501 gcccgagcgc atgcccccgc acggtcatcg cgtcgagcac cgccaccgtg ccggtgtcgg
     1561 tgacgaaggt cgtctccagc acgttggtct cgggcaggta gcggcgcgac accgtgtgcg
     1621 cgacggtcgg cgccagcgtg gcgcgcccgc cgcgctcggc gtcgagcagg gcggcgaaca
     1681 cgctggggga gtcaaggtcg ggcagggcaa gccagtcgat ggacccgtcg tcggcgacca
     1741 gcgcgaccgt gcggccggcg ccgatggccg cgtaggcccg cagcggggcg taaccgtcga
     1801 cccgcgccgc ggcggtgtgg gcggtcgccg ggctgggtga ggtcatcgca tcggacacta
     1861 cgacctgtcg ccccccgtgg ttcacatccg cgctcgggcg agttgaaggc tcagcgcagc
     1921 cgtaggagca ccttgccggc gatggaggca tcggccaggg cggtgagggc gtcgttggcc
     1981 gcctcgagcg ggacctcacg gccgatcccg acccgcagcc tgccttcggc ggcggcggtg
     2041 agcgcggcgc taatgcccgc ccgacgctcg tcgcgggtgg cctggacgcc ggcataggtg
     2101 agcagctgga tctggttgcg gtagagcggg agcaggtcga gcgtggcctc gctgcccgcg
     2161 ctcgcgccga agagcacgag ccggcctcgg gcggccatca ccgtcagcac ggcggaggtg
     2221 aacgcaccac cgagcgggtc gagcacgacc gtgggattga tgtctcggac cgccgcggcc
     2281 agaccgggcg cgtcggcgac cacggcgtcc ttggccccca tgtcgcggat ggccgtcgcc
     2341 ttggccgccg acctggtctg cccccacacc cgagcccccg tcgaggcggc gtaggagatc
     2401 gcggacaggc ccacggcgcc gctgccaccg agcacgagca cccggtcatc gggtccgata
     2461 tgagcggttt gtacgacgcg gtaggcggtc agcccgacga tgcccatcgc cgcggcgtcc
     2521 cgcagcgaga cgccggcggg cacctccacc accgcgtcac gcggtacgac ggcagcctcg
     2581 gcgaagacgc cgtcgcgggt ggtgccgagc ccctcaccgg cgatcacgac cggcctgccg
     2641 tcgagcaggc ccgacgcctc gcctccgagc gtgcgcggca gcggcgcgtc cggcgcgacc
     2701 cggccggcgg cgacatagcc gtcaaagggg ttgaccccgc cataggccag ctcgacgagc
     2761 acctcgtcgg ccgacggcgt gggcagctcg acgtcttgga cgagcagtgg ctgaccatgc
     2821 gctgctagcc gtgcggcccg gatgctcatg gaacggacat taccaacggg atcaagcggt
     2881 catgatcacc gcggccagcc acaggccggc ggccagcagc ccggctccca gctcgatcag
     2941 cagcgagacg cccacggcca tcaccgccgc ccgggccgac ggccaggcga gctcgtggct
     3001 gcctagccgc agccgttccg cgacgtacat cccggcgacg aacccgatca ccgcgccgat
     3061 caccgggatg acgaagaacc cgacgatcgc cagcaccgtg gccgccgccg tcgacgccca
     3121 gggcacgcca ccggcgcgca ggcgccgggc gggtacgacg tacttgacca gactgcccgc
     3181 cacgatgagc gccgcggcgc ccgccaggac cgtccacgcc gtcggggtgc ctaccacgat
     3241 cgcccagacc gcgatcgccc cgagcacgat cggcaacccg ggcagcaggg gtacgacgac
     3301 gccgagcagg ccgagcagga tcaccagccc tacgagcacg aggccgccgg tgtccactcg
     3361 ctcatcctgg cgcccggagg gcatccggag gggccgcctt cagcgccgta gacggactga
     3421 gggccccggc cggcggccgg ggccctcatg caggctgggt gggtgtcagc gttcgtcggc
     3481 cggagcgatc gccggggtgc tggcgagccg gtcggactcg tcgtggatct cggcggccat
     3541 cgtcttgagg gtgggctcgt agcgccgggc gtggtgggca cagaagagca gctcgccgcc
     3601 tgaggccagg atgacccgta cgtaggcctg ggcgccgcac cggtcacaac ggtcggcagc
     3661 ggtcagctcg tgaactgggg caagggctgc ggtcacgtcg tctcgtctcc tcgtcgcgtc
     3721 tgctacccgt ggcaacatcc aaccacccct tgtcgttccc actgtgtcgg ggccaacacg
     3781 tgtcccggtc agttctcttt ccgtgaccgc ggtgtgactg agccagccgt gcggcttcgg
     3841 cgcgcccaca ggcggcctgt ccgcggcccc aggtaacctg ccaggcgctg gacaagccgg
     3901 cacctccttc cgttcgacga gccttccgag gagcaagcac cgcgtgagcg cgcagcccag
     3961 cgagcccctg cccaaggtcg acggctacac cgcgcggcac ctgtcggtgc tcgacgggct
     4021 cgaggccgtg cgcaagcgcc cgggcatgta catcggctcg accgactcgc gcgggctgat
     4081 gcactgcctc tgggagatca tcgacaacgc cgtcgacgag gcgctgggcg ggcactgcca
     4141 gcgcatcgag gtcgcgctgc acgccgacgg gtcggtcgag gtgcgcgacg acggccgggg
     4201 cgtgccgatc gacatcgagc ccaagaccgg gctgcccggt gtccaggtgg tcttcaccaa
     4261 gctgcacgcc ggcggcaagt tcggcggcgg ctcctacacc gcctctggtg gcctgcacgg
     4321 ggtcggcgcc agcgtggtca acgccctggc ggcgcgcctc gacgtcgagg tggaccgcgg
     4381 cggcaaggtc cacgcgatga gctttcggcg cggcgagccg ggggtctacg acgacggccg
     4441 gccgggcccg agccccgagt cgccgttcgc gccgttcacc gccaggtccg agctgcgggt
     4501 ggtcggcaag gtcaagcgtg gcgtcaccgg cacccgggtc cgctactggc ccgaccggca
     4561 gatcttcctg cccgacgcca cgttctcctt cgacgagctg gtcacccggg cccgccagac
     4621 ctcctacctc gtgcccggcc tgacgctggt ggtccgcgac gagcggcctc ggcccgacgc
     4681 cgacgggtcg gtcgaggaga cgttccacca cgaggagggc atcggcgagt tcgccgagtt
     4741 cctcgccccc gacccggcgg tcaccgacgt ctggcggctg cgcggcacgg gccacttcaa
     4801 ggagaccgtg ccggtgctcg acgagcgcgg ccacatgcgc ccggccgagg tcgaacgcga
     4861 gtgcgtcgtc gacgtcgcgc tgcgctgggg caccggctac gacacccggc tgtgctcgtt
     4921 cgtcaacatc atcgccaccc ccaagggcgg cacccacgtc gccgggttcg agcacgccct
     4981 gctcaagacg ttcaagaagg cgatcgaggc caactcgcgg cggctaaaga ccggcaagga
     5041 cgacaagctc gacaaggacg acgtcctcga ggggctcacg acggtggtca ccgtccggct
     5101 ggccgagccg cagttcgagg gccagaccaa ggaggtgctg ggcaccgccg cggtccgcgg
     5161 catcgtggcc cgggtggtcg agcgcgagct cacggcgcgg ctgacctcga ccaagcgcgg
     5221 tgacaaggcg caggccgcca ccgtgctcga caaggtcgtc gcggcgatga agacccgggt
     5281 cgcggcccgc atccacaagg agacccagcg gcgcaagaac gcgctggaga cctcgtcgtt
     5341 gccggccaag cttgccgact gccgcagcga cgacgtggcc cgcaccgagt tgttcatcgt
     5401 cgagggcgac agcgccctgg gcaccgccaa gttcgcccgc agctccgagt tccaggcgct
     5461 gctgccgatc cgcggcaaga tcctcaacgt gcagaaggcc tcgcccggcg acatgttcaa
     5521 gaacgccgag tgcgccgcga tcatccaggt ggtcggtgcc ggatcggggc gcacgttcga
     5581 cctcgaggcg gcccgctacg gcaaggtcgt gttcatgtcc gacgccgacg tcgacggcgc
     5641 gcacatccgc accctgctgc tcaccctgtt cttccgctac atgcgcccga tggtcgaggc
     5701 cggccgggtc ttcgccgccg tacccccgct gcaccgcgtc gaggtgatca acccgggcgg
     5761 gaagaagaac gacgtcgtct acacctactc cgacgccgag ctgcgggccg tgctcgccga
     5821 cctggcccgc aagggcaagc gctacaagga gccgatccag cgctacaagg gtcttggtga
     5881 gatggacgcc gaccagctgg ccgaaaccac gatggacccg cgccaccgca cgctgcgccg
     5941 ggtgaccatg accgacgccg aagcggccga gcgggtcttc gagctgctca tgggcaacga
     6001 cgtcgcgccc cgccgtgagt tcatcgtggc cggcgccgac gagctcgacc gggctcgcgt
     6061 cgacgcctga ggctcactca ccccgtcacc gggggcccgc tccccggcgg gtcacgggcg
     6121 cggggcgtgc tggcgaccca gcagcggcgc caggcaccac gacgcggcca gcatgacggc
     6181 agcgactgcg agcatcacgg cgactgcggt gaccacgatg gtcatcccgt ctgacccgtc
     6241 tgacccgccc gggctcgggg ccgaggcagc ccaccgggcg agcccgccgg cggtcgcgac
     6301 cgggatcgct gcgatcacga cgtaggccgc acgccgtgac agtgccggtg cggccctgcg
     6361 ggccacgagg agcaccgtga aggtcaggcc ctgggtcacc acgccggcca ccatgccgac
     6421 ggtggcgccg gcgagcagac cgaacagcgc ataggcctgc aggccgacca cgatcgtccc
     6481 gacggcgcca ccgatcaccg cgccagccag gagccccacg ccgagcaggc gcgcgagcgt
     6541 gcgatagccc gccatcgggt ccggataccg cgcctccacc ccgtccgtca tgggggccac
     6601 ttgatcacga gtgtgccggg atgatcaagg cttcgtgggg gcgtggtcag taggaccgcg
     6661 gcgatctcgg ctttcgccgg ccggcgccgg ctgcgatgaa gatctcgcgg tgctgtgcat
     6721 cccggcgtac gttggcccgt gcgacgacat gctgtcgtac ggcgacctgc gcgcgggtga
     6781 gcgcgatgct gaggggagca tcaggtgtcc agctggaacg gcatgaccta cgagggcaag
     6841 gacacgctgc tgcgcgtcgt tcgtgaggag gccagatcgt tctttgccct cgtcagcgac
     6901 ccgcaggcgt gggaggcacc gacccagagc acgacctggc aggtgcgtga cgtcgtcggg
     6961 cacctggttg acacgacgga gtcctacttc ccggccttcg acgcggcccg ggcccacgcc
     7021 gaggtcgagc cggcctatgg cctgcctggc atggcggccc gggtcgacga gcgcgccgtg
     7081 gctctgcgct cggtcccgcg cgaggagctg ctcgagcgct tgcgcggcga cttcgccaag
     7141 atgatggaga tcctgggcgc gctcggcccc gacgagtggg gcggcctgct ggtgccgcac
     7201 ttctacatgg gcccgctgcc ggccttcttc taccccgcgt tccagctgat ggactacggc
     7261 gtgcactcct gggacctgcg ccagagcact gggcgggccc acgggttcag cggcgaggcc
     7321 gccgacctgc tggtgccgtt catgttcgtg ctgtggcagg ccaccgccac gtgcgggccc
     7381 gacaccgaac ccacgacgct ggggatccgg gtgacgagcg gacccaacgc cggcgcgacc
     7441 cgggtcagcg tcggacccga gggcatctct ttcgagcccg gcgagatcac cgacctggcg
     7501 acggtgctcg agttcgaccc gggcagcttc gtgctgaccg ccttcggacg cagcaacgcc
     7561 ggcacgatcc gcggcgaccg tgtcacggcc gaccgctacc tcaacctgtt cttccgcatc
     7621 tgagccgcct cgcggccgcc tcactccagg aggcacaacc atggctgaca tcgacctgtc
     7681 cgcgatcgag gccgagcgcg cccgcatccg cgagacctac ctgcgtccca ccggcagccg
     7741 tccggcgtca tctgcacagg gactgcacca cttcgccgtc gtctgctccg acgtcgagcg
     7801 caccgtgaac ttctaccagg gcctgctcga gttcccgctg accgagattt tcgagaaccg
     7861 cgactacaag ggctccaacc acttcttctt cgacgtgggg cacggcaacc tgttggcctt
     7921 cttcgacttt cccggcctcg atgtcggccc ctacgccgag gtgctcggcg gactgcacca
     7981 catcgcgatc tcggtcgagc gccccacctg ggagcggctg agaggcaagc tcgacgccgc
     8041 gggggtcgac tacctcgagg agagcgggtc ctcgctctac ttccgtgacc ccgacgggac
     8101 acgcctcgag ctgctggcgg acccgctcgg tcagatgtat ggcctgaccg tgctgtgatc
     8161 taggtgacct gctgaagccg gctgggcagc aggctgcgca gggcgaccgc gggcttgccg
     8221 cacgactcgg gccgcgcctg cgccgactcg acctcgtgca ccgcgaccgt ccagcggcgc
     8281 ccatctcggt gggtcacctc gaccagccag cctggcccgc cgtcacctcc ggcgtcccag
     8341 accgaagccg cgtcgaggtc gtcgacgccc tcgatgccga gcttggcacg caccagcgcc
     8401 tcgccgacct gggccggctt gctgaacgcg ctgcggccgc gatagccgtg cgcggcgagc
     8461 cgaccagcgt cggccgcgac cagcaggtcg accgcctgcg ggtcggtcag ccgtccgtgc
     8521 acgctgccct gaggcagcac cacggcggtg ggggagaacc ggtgcccgcc cagatgactg
     8581 gtctcccaga cccgccccgc gtcggcacca ccgaacgcgg cggcggcgtg cgcggccagc
     8641 gggcggccgc cgatcgcgca gcaccggtcg cggcgagcgt tggtgcacac cgccagcacc
     8701 ggcgggctgg gttcaccgac cgagccgggg ccctgggtgg ccagcctcgt caggtcgagg
     8761 tcgagcagct cccgcgggtc gcgcatgcgc agcagccgca gcgcgctcgc gccggggcgg
     8821 gtgaaggcca agaagaccga ccgcggggcg ccccggtcgg ggtggcggcc cggacggcgg
     8881 accagcccca gtcgtacgcc ggcggccgcg gccgcggcgg ccagtggatg ggccacctca
     8941 ccgggcaggt gtgactgggt gggtgcctcc cgcccccagg ggccgggctg ctcgaccagc
     9001 agccaggccg cggcgaccgg ggcggtgccg ggcaacggtt caccgacgtc ggcgctgacc
     9061 agcgagcagc gcggccctag ctccacgcgg ccgccggccc cgcgaccccg gcgatcggca
     9121 tcgccgcggg tgtgcccgac ccgtcacggc ggccggtggc agcgggcagc tcgaccgcga
     9181 ccccgctggc cgcggcggcc cgagccggcc ctggcccggc ccacgccagc accagcgcgt
     9241 cctcgccctt gaggaaccgg tggcagcgca caccgccggt gccgcggccc ttggccggga
     9301 actcggtgaa cggggccacc ttgaccgatc ccgcgtcggt gccgggcagg gcggccgagc
     9361 tgcctgacac ggtcacgacg acggcatccg cgccggggtc gaccgcgccg aagaacacca
     9421 cctggacgcc cgcactgcac cgcacgccgg cgaccccgcc ggccccgcgg ccctgcgggc
     9481 gcactcccgc ggccgaaaac cgcaggagat gggcgtcgct ggtgacgaac accaggtcct
     9541 cgtcgccggt gcgcagctcg gcggccccga tcacccggtc gccgggcttg agcccgatga
     9601 cctcccactc atgcttgttg gctgggtagt cgggcgcgac ccgcttgacg accccggcgg
     9661 cggtgccgag cacgacgccg ggggagtcgg tcgacaggct gaccagcgtg agcaccgact
     9721 cgtcggcgtc gagagcgagc agcgccccga gcggcagccc gcccgacagg ttgggggcgc
     9781 tcgcggtcgg cgggcgggtc ggcagctcga gcaccgacag ccggcgcatc cggccggccg
     9841 aggtcagcac ggccacctcg ccgcgggcgg tggcccgcac cgcggagacg atgacgtcgt
     9901 gcttggcacg cccagtggag acgggcatcg gggcgtcgtc gctggtgcgg gccaccagcc
     9961 cggtcgagga gagcagcacc cagcacggat cgtcggagac ctcgagcggc acgacggcgg
    10021 tggccggcgc cccggcggcc tcgagcagca cggtccgtcg cggggtgccg taggtcttgg
    10081 ccaccgcggc cagctcgtcg gagacgacct cacgaagccg ggcatcgtca ccgaggatcg
    10141 cgtccagggc ctcgatgtcg gcctcgagcg ctgacttctc cttctccagc tcgatgcgcg
    10201 agaacttggt cagccggcgc agctgcatct cgaggatgta gtcggcctgc acctcggaca
    10261 ggtcgaacac cgacctgagc cgctcgcgtg ccgccccggc gtcgtcgctg gagcggatca
    10321 ccgcgatgac ctcgtcgatg tcgaggatcg cgatgagcag cccctcgacc ttgtgcagcc
    10381 ggtcggccgc cttgcggcgg cggaagagcg agcgacgccg tacgacgtcg aggcggtgat
    10441 cgacgtagac ggtcagcagc tcgcgcagcc cgagggtgcg cggctgaccg tcgaccaggg
    10501 ccacgttgtt gatgccgaag gagtcctcca tcggcgtgag cttgtagagc ttggccagca
    10561 ccgcctcggg gttgaagccg ttcttgacct cgatgaccag ccgcagcccc ttgtcgcggt
    10621 cggtgaggtc ggtgacgtcg gagatgccga gcagcttctt ggcctggacc aggtccttga
    10681 tcttctcgat gaccttctcg ggcccgacgg tgtagggcag ctcggtgacc acgatgccct
    10741 tcttgcgggc ggtcaccgac tcgatgcggg cggtggcgcg gatccggaac gacccgcgac
    10801 cggtctcgta ggcctcgcgg atgccgtcga gcccgacgat ggtgccgccg gtgggcaggt
    10861 cggggccggg tacgaaccgg accagctcgt ccaacgtcgc ctcgggccgc ttgatcaggt
    10921 gccgggccgc ggcgatgacc tcgatgaggt tgtgcggggc catgttggtg gccatgccga
    10981 ccgcgatgcc ggaggagccg ttgacgagca gattgggata ctgggcgggc aggatcaagg
    11041 gctcgtcgcg cgtgccgtcg tagttgagac gcatgtccac
//
LOCUS       DATIBB010000040         6507 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_1464001, whole genome shotgun sequence.
ACCESSION   DATIBB010000040 DATIBB010000000
VERSION     DATIBB010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 6507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6507
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1464001"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(3..1415)
                     /gene="lysA"
                     /locus_tag="VLC50_01355"
     CDS             complement(3..1415)
                     /gene="lysA"
                     /locus_tag="VLC50_01355"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018216801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HSF26141.1"
                     /translation="MRAHEAGALHADVGHPAPPWLRVPDDVNALLPALWPAHVCRGDD
                     GVLAVAGVPVTELAREFATPAYVVDEDDLRARARAFRDAFAEAFDGRAAVYYASKAFL
                     TTAVARWVVEEGLNLDVCTGGELAVALRAGVSGDRIGLHGNNKSLAELEAALDAGVGR
                     VIVDCFEEIDRLAELAARRGVRAPVLVRVTVGVEAHTHEYIATAHEDQKFGFSLAGGQ
                     AAEAVRRVLAAPSLELRGLHSHIGSQIFDDAGFEVSARRVLGLHAWVRDEYGVELPEL
                     DLGGGFGIAYTSQDDPASAAEHAHRLREIVDAECRAAGLAMPVVSVEPGRAIAGPAVF
                     TLYEVGVVKPVEVAPGLVRTYVAVDGGMSDNVRPALYDASYSCTLASRASAAEPTLAR
                     VVGKHCESGDIVVKDEFLPADVAPGDLIAVPGTGAYCRSMASSYNHVPRPPVVAVRNG
                     TARVIVRRETVDDLLRLDVE"
     gene            complement(1419..3080)
                     /gene="argS"
                     /locus_tag="VLC50_01360"
     CDS             complement(1419..3080)
                     /gene="argS"
                     /locus_tag="VLC50_01360"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018784844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HSF26142.1"
                     /translation="MTPAQLSEAVRQAVQQAVDAGLLTVAVPAEVRVERPRNRDHGDY
                     ATNVAMQLAKSAGRPPREVAEVIAGQLSAAAGVKSVEVAGPGFLNITLDAAAQGDLAR
                     VVVLAGESFGRSDTLAGERVNLEFVSANPTGPVHIGAVRWAAVGDALGRVLDAAGAAV
                     TREYYFNDAGAQIDRFAASLLAAAKGQPTPEDGYAGAYIGEIAAEVLARRPETLGLPD
                     DEALAVFREEGVALMFVEIKTSLHDFGVDFDVYFNEKSLHEAGKLAEALARLTAQGRV
                     FEADGATWLRTTDFGDDKDRVLRKSDGDWTYFAADAAYYLDKRDRGFDRCIYMLGADH
                     HGYVGRLRAVASCFGDDPDRTLEVLIGQLVNVVKDGAPVRMSKRAGNVVLLADLVEAI
                     GVDATRYALARYSTDSPIDLDLDLWSRRTQDNPVYYVQYAHARLASILRNAAELGVVR
                     VAVDGFHPELLDHDREGDLLGALGEFPRVVAQAAELREPHRIARYLEDLAGTFHKFYD
                     ECRVLPRGDEEVTDVMQARLWLVEAARVVLANGLRLLGVSAPERM"
     gene            complement(3077..3463)
                     /locus_tag="VLC50_01365"
     CDS             complement(3077..3463)
                     /locus_tag="VLC50_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013131102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSF26143.1"
                     /translation="MQPRRGRVLVVDDSPQIRALIRVNLELEGFEVITLADGAEALER
                     VQAIAPDVMTIDVKMPRLDGFETVQRLRADPATASLPIAVVTACAQATDRQRGQEVGV
                     DAYITKPFDPAVLVAEVRRLVDTLGR"
     gene            3568..3639
                     /locus_tag="VLC50_01370"
     tRNA            3568..3639
                     /locus_tag="VLC50_01370"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3601..3603,aa:Arg,seq:ccg)
     gene            complement(3677..5302)
                     /locus_tag="VLC50_01375"
     CDS             complement(3677..5302)
                     /locus_tag="VLC50_01375"
                     /inference="COORDINATES: protein motif:HMM:NF014741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HSF26144.1"
                     /translation="MDATQKTVVKTASAKAGPWVQEGVALPEWASWPAGPELGAMLAG
                     LDVARLDEPALVEAMAAFERQASWCRAGQLVAIAALAGHRSPVEAAPAPDGSGQSWAD
                     RGVARHPQVSEFVMQEVAARLSTSKHDAWARVGDALDLLEVDGLRATFAALTLGVIDA
                     RTAHIVAETARGFDESADADELRAAFEERLLPKVVDRTPRQARDEARRLVLELDPQGA
                     EERAAGARAARKVILEPAPDATAWLSALLPAEGAVAVYTCLDLLARHAHQPDDQRSID
                     QRRADALVELVTSHPLITGTCWCTGSCATHPVPAAGSTAEPGSAFTPRAGQSGIGCAA
                     VSTEATRTPAGGIGASGRRRHLVHLSVPAFTMLGAGTAPAFLHGYGHVPLAVAEAIAA
                     DAQWRRLLTDPFTGTVIGAEVTAHDPPPSMADLVNARYQSCGFGPCTREAEACDLDHA
                     DPHPHGPTSPTNLTPECRPEHRMKTLSTGWTLHRDGGEFVWTSPTGHCYPIEPAQVGT
                     ILGAIYRGTYRSELDEPPDDQPSDHEPLEDAEA"
     gene            complement(5530..6135)
                     /locus_tag="VLC50_01380"
     CDS             complement(5530..6135)
                     /locus_tag="VLC50_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012690357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HSF26145.1"
                     /translation="MRLTTNTQISVDGVMQGNGGRHPDLDPGFERGGWARPLFESEAM
                     AYVEEVYRRADAFLLGRRTYDLFAGYWGVRRDFDNPIVSALNTKPKYVASTSLRSPEW
                     AGTTVLSDDLATAVRELKDRPSGELQVHGSGALTRWLLALDLVDEINLLVCPVIVGQG
                     ARLFPEDGPDHALDLVASRPFPGGIVLQTYLPSGRPRYAAE"
     gene            complement(6266..>6507)
                     /locus_tag="VLC50_01385"
     CDS             complement(6266..>6507)
                     /locus_tag="VLC50_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017309117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HSF26146.1"
                     /translation="RLRREIEHEWVLGIAGVLSIVLAIFLVITPGAGALVITWAIGWY
                     AIVLGVLELVLAWEVRRETNQPRSSARPGTPRPAM"
BASE COUNT          915 a         2335 c         2353 g          904 t
ORIGIN      
        1 gatcactcga cgtccaggcg cagcaggtcg tccaccgtct cgcggcggac gatcacccgt
       61 gcggtgccat tgcgcaccgc gaccaccggc ggacgcggca cgtggttgta gctgctggcc
      121 atgctccggc agtaggcgcc ggtgcccggc accgcgatca ggtcgcccgg cgccacgtcg
      181 gccggcagga actcgtcctt gaccacgatg tcaccggact cgcagtgctt gccgaccacc
      241 cgggccaggg tcggctcggc ggccgaggcc cgcgaggcga gggtgcacga gtaggacgcg
      301 tcgtagagcg ccggccgcac gttgtcgctc atcccgccgt cgacggcgac gtaggtgcgg
      361 accaggccgg gggccacctc gaccggcttg accacgccca cctcatagag cgtgaacacc
      421 gccggcccgg cgatcgcccg gccgggctcc accgacacga ccggcatcgc gagcccggcg
      481 gctcggcact cggcgtcgac gatctcgcgc agccggtggg cgtgctcggc cgcgctcgcc
      541 gggtcgtcct ggctggtgta ggcgatgccg aagccgccgc cgaggtcgag ctcgggcagc
      601 tcgacgccgt actcgtcgcg gacccaggcg tgcagcccga gcacccgccg ggcggagacc
      661 tcgaagccgg cgtcgtcgaa gatctgcgag ccgatgtgcg agtgcagccc gcgcagctcg
      721 agcgagggtg cggccagcac tcgccgtacc gcctcggcgg cctgcccgcc cgccagcgag
      781 aagccgaact tctggtcctc gtgcgcggtg gcgatgtatt cgtgggtgtg ggcctcgacc
      841 ccgaccgtga cccgcaccag caccggtgcc cgcaccccgc gccgggccgc cagctcggcc
      901 agccggtcga tctcctcgaa gcagtcgacg atgacccggc cgacgccggc gtcgagggcg
      961 gcctcgagct cggcgagcga cttgttgttg ccgtgcaggc cgatccggtc tccgctcacg
     1021 ccggcgcgca gcgcgacggc cagctcgccg ccggtgcaca cgtcgaggtt gagcccctcc
     1081 tcgaccaccc agcgggccac cgcggtcgtg aggaaggcct tgctggcgta gtagaccgcg
     1141 gcccgcccgt cgaaggcctc ggcgaacgcg tcgcggaagg cccgggcccg agcccgcagg
     1201 tcatcctcgt cgacgacgta ggccggggtg gcgaactcgc gggccagctc ggtgaccggc
     1261 accccggcca ccgcgagcac cccgtcgtcg ccgcggcaca cgtgcgccgg ccagagcgcg
     1321 ggcagcagcg cgttgacgtc gtcggggacc cgaagccagg gcggggcagg gtggccgacg
     1381 tcggcgtgca gggcgccggc ctcgtgggct cgcatgggct acatccgttc gggagcgctg
     1441 acgccgagca ggcgcagacc gttggccagt acgacgcgcg cggcctcgac cagccagagc
     1501 cgggcctgca tcacgtcggt gacctcctcg tcaccgcgtg ggagaacccg gcactcgtcg
     1561 tagaacttgt ggaacgtgcc ggcgaggtct tcgaggtagc gcgcgatgcg gtgcggttcg
     1621 cgcagctcgg cggcctgggc gaccacgcgg gggaactcgc cgagcgcgcc gagcaggtcg
     1681 ccctcgcggt cgtggtcgag cagctcgggg tggaagccgt cgacggcgac ccgcaccacg
     1741 ccgagctcgg cggcgttgcg caggatcgag gccagccggg cgtgggcgta ctgcacgtag
     1801 tagacggggt tgtcctgggt gcgccgcgac cacaggtcga ggtcgaggtc gatcggcgag
     1861 tcggtgctgt agcgggccag cgcataacgc gtggcgtcga ccccgatggc ctcgaccagg
     1921 tcggcgagca gcacgacgtt gccggcccgc ttgctcatcc ggaccggcgc gccgtccttg
     1981 accacgttga ccagctggcc gatgagcacc tcaagcgtgc ggtcggggtc gtcgccgaag
     2041 cacgacgcga ccgcgcggag ccggccgacg tagccgtggt ggtcggcacc gagcatgtag
     2101 atgcaccggt cgaaaccgcg gtcacgcttg tcgaggtagt aggcggcgtc ggcggcgaag
     2161 taggtccagt cgccgtcgga cttgcgcagc acccggtcct tgtcgtcgcc gaagtcggtg
     2221 gtgcgcagcc aggtcgcccc gtcggcctcg aagacccgac cctgggcggt cagccgggcc
     2281 agcgcctcgg cgagcttgcc cgcctcgtgc aacgatttct cgttgaagta gacgtcgaag
     2341 tcgaccccga agtcgtgcag gctcgtcttg atctcgacga acatcagcgc gacgccctcc
     2401 tcgcggaaca ccgcgagcgc ctcgtcgtcg ggcagcccga gcgtctcggg gcggcgggcc
     2461 agcacctcgg ctgcgatctc gccgatgtag gcgccggcgt agccgtcctc gggggtcggc
     2521 tggcccttgg cggcggccag caggctcgcc gcgaaccggt cgatctgggc tccggcgtcg
     2581 ttgaagtagt actcccgggt gaccgcggcc ccggccgcgt cgaggacccg gccgagcgcg
     2641 tcgccgacgg cggcccagcg gaccgcgccg atgtgcaccg gaccggtggg gttggccgag
     2701 acgaactcga ggttgacccg ctcaccggcg agggtgtcgc tgcggccaaa gctctccccg
     2761 gcgagcacca cgacccgggc gaggtcgccc tgagccgcgg cgtcgagggt gatgttgagg
     2821 aagccggggc cggccacctc gacgctcttg acccctgcgg ccgccgacag ctgcccggcg
     2881 atgacctcgg ccacctcgcg cggcggacgc ccggccgact tggccagctg catcgccacg
     2941 ttggtggcgt agtcgccgtg gtcgcggttg cggggacgct cgacgcggac ctccgcgggg
     3001 acggcaacgg tcagcaggcc ggcgtcgacg gcctgctgca ccgcctgacg gactgcctca
     3061 gaaagctgtg cgggggtcac cgaccaaggg tatcgaccag ccgtcgcacc tctgccacga
     3121 gcaccgcggg gtcgaagggc ttggtgatgt aggcgtcaac ccccacctcc tggccgcgtt
     3181 ggcgatcggt cgcctgcgcg cacgcggtga ccaccgcgat cggcaggctg gcggtggccg
     3241 gatcggcgcg cagccgctgc acggtctcga agccgtcgag ccggggcatc ttgacgtcga
     3301 tggtcatgac gtcgggggcg atcgcctgca cccgctcgag cgcctcggct ccgtcggcca
     3361 acgtgatcac ctcgaagccc tcgagctcaa ggttgacccg gatgagcgct cggatctggg
     3421 ggctgtcgtc gacaaccagg acccggccac ggcgcggctg cacgggccga gggtagcggt
     3481 gcggcacgtg tcggcagccg ctttggaaga cgtcgtacgg cgacggggcg ggtgccgctc
     3541 ggctgctaat ctgtgcgccg aacccgagcc cccgtagctc aggggataga gcaccgccct
     3601 ccggaggcgg gtgcgcaggt tcgaatcctg ccgggggcac tcacagattt tcctgaccag
     3661 cagcgccccg catccctcag gcttcggcgt cctcgagcgg ttcatggtcg ctcggctggt
     3721 catcgggtgg ctcgtcgagc tcgctgcggt aggtgccgcg gtagatcgcc ccgaggatgg
     3781 tgccgacctg ggccggctcg atcgggtagc agtgaccggt cggggacgtc cagacgaact
     3841 cgccgccgtc gcggtgcaga gtccacccgg tggacagggt cttcatccgg tgctcgggtc
     3901 ggcactcggg ggtcaggttg gtcgggctgg tcggcccgtg cgggtggggg tcggcgtggt
     3961 cgaggtcgca cgcctcggcc tcgcgggtgc agggtccgaa gccgcaggac tgatatcggg
     4021 cgttgaccag gtcagccatc gacggcggcg gatcgtgcgc tgtgacctcg gctccgatca
     4081 ccgtgcccgt gaacgggtcg gtgagcaagc ggcgccattg cgcgtcggcg gcgatggcct
     4141 cggccaccgc cagcggcacg tgaccgtaac cgtgcaggaa cgccggggcc gtgccggctc
     4201 ccagcatcgt gaacgccggc acgctcaggt gcaccaggtg gcggcgccga cccgacgcgc
     4261 cgatgcctcc cgcgggagtg cgagttgcct cggtcgagac cgcggcgcac ccaatgcctg
     4321 actggccggc gcggggagtg aaggccgacc caggctcggc cgttgagcca gccgcgggca
     4381 cggggtgggt ggcgcagctg ccggtgcacc aacacgtccc ggtgatcaac gggtggctgg
     4441 tgaccagctc gacgagcgca tcggcgcgcc gctggtcgat gctgcgctga tcatcgggtt
     4501 ggtgggcgtg gcgggccagc aggtcaagac acgtgtagac ggcgaccgca ccctcggccg
     4561 gcagcagcgc cgacagccag gcggtggcat ctggggcggg ctcgaggatc accttgcggg
     4621 ccgcccgggc gccggccgcg cgctcctcgg cgccctgcgg gtcgagctcg aggaccagcc
     4681 ggcgcgcctc gtctcgggcc tgccgcggcg tgcgatccac caccttgggc agcagccgct
     4741 cctcgaacgc cgcgcgcagc tcgtcggcat cggccgactc gtcgaagccg cgggccgtct
     4801 cggccacgat gtgcgcggtg cgggcgtcga tcacccccaa cgtcagcgcg gcgaacgtcg
     4861 cgcgcagccc gtcgacctcg agcaggtcta acgcgtcacc gacccgcgcc cacgcgtcgt
     4921 gcttggacgt cgacagccgg gcggccacct cctgcatcac gaactcgctc acctgcgggt
     4981 gacgggcgac cccacgatcc gcccaggact gcccggaccc gtccggggcc ggcgccgcct
     5041 ccaccggtga gcggtgaccg gccagcgcag cgatcgcgac cagctgtccc gcccggcacc
     5101 aggacgcctg ccgctcgaac gctgccatcg cctcgaccag cgccggctcg tcgaggcgcg
     5161 ccacgtcgag cccggccagc atggcgccca gctccggacc cgccggccac gacgcccact
     5221 cgggcagtgc caccccctcc tgcacccagg gacccgcctt ggcagacgcc gtcttgacca
     5281 ccgtcttttg cgtcgcgtcc aaggcgacgc tccgcgccca gcgcgtggca cccgaccacg
     5341 accgggagct gggcggcgcg cccccaccga tcaacgatcc ccgttgttcg aacatgtgta
     5401 cgagtctatg tggtcgaggt gcgggtgtcc agcatttcgc cgtacatcat tgtttgcacg
     5461 ccaagaatgc ggcgacccaa gcgtcggact cgatcgagat gcctcgcgag aggggcggcg
     5521 gcaggcttct cactccgctg cgtaacgcgg gcgccccgag ggcaggtagg tctgcaggac
     5581 gattccaccg gggaacgggc gcgacgccac gaggtcgagg gcatggtcgg ggccgtcctc
     5641 ggggaacagc cgcgcaccct gcccgacgat caccgggcag accaagagat tgatctcgtc
     5701 gacgaggtca agcgcgagca accatcgcgt cagggctccg ctgccgtgca cctggagctc
     5761 cccgctgggc cggtccttga gctcgcggac cgcggtagcg agatcatcgg agaggaccgt
     5821 ggtgcctgcc cactctggcg accgcagcga cgtcgacgcg acgtatttgg gcttggtgtt
     5881 gagcgcactg acgatggggt tgtcgaagtc cctgcgcacg ccccagtagc cggcgaacag
     5941 gtcgtaggtc cggcggccga gcaggaatgc gtcggctcgt cggtagacct cctccacgta
     6001 ggccatcgct tcgctctcga acagcggcct cgcccacccg cctcgctcaa agcctgggtc
     6061 gaggtcaggg tggcggccac cgtttccctg catcacgccg tcgacggaga tctgcgtgtt
     6121 cgtcgtgagc ctcatgatcc cgcccacctc gcgtcgcccg ccggccctcg aagccggatt
     6181 ggtccggacg ggtacggcgg cggctccgct ggccaccgca ccgccctcgg cacgagtgat
     6241 cgcgcagtca tcacgccact cgggctcaca ttgctgggcg tggcgtcccc ggccgggccg
     6301 acgaccgtgg ctggttcgtc tcgcgtcgta cctcccacgc cagcacgagc tccaggacgc
     6361 cgagcacgat ggcgtaccag ccgatcgccc acgtgatcac cagtgcaccc gcgcccggcg
     6421 tgatgacgag gaagatggcc agcacgatcg agagcacccc ggcgatgccg agcacccact
     6481 cgtgctctat ctcccggcga agccgga
//
LOCUS       DATIBB010000041        17768 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_807013, whole genome shotgun sequence.
ACCESSION   DATIBB010000041 DATIBB010000000
VERSION     DATIBB010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 17768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17768
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_807013"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..442
                     /locus_tag="VLC50_01390"
     CDS             <1..442
                     /locus_tag="VLC50_01390"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HSF26147.1"
                     /translation="RAAERELARVARTGSRLAVAVIDLDGFKKVNDTLGHAAGDQLLV
                     TIATAWSKVLRPTDVLARHGGDEFVLLLPGAGAAEATTVLHRLLRANADIGWSYGLTA
                     YEPGDTLDRLLARADVRLYDAKAARTPPTSSPLCAQVSPGAMAV"
     gene            complement(520..1332)
                     /locus_tag="VLC50_01395"
     CDS             complement(520..1332)
                     /locus_tag="VLC50_01395"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2249 domain-containing protein"
                     /protein_id="HSF26148.1"
                     /translation="MTTAPAIGADVAELPPGAAETIAAIKSHHAQLSDDFAHGVDRVR
                     EAVERLAPVTGARDDLLGFITAEVLPHAAAEEATLYGAGEHLPASGLLVKGMIAEHRV
                     IMKLTDALAAASTPAETVAAAAAVHAVFEAHLAKENDLLLPALVAGGVDIAALLEGMH
                     DLLGGHAEEVHGGCGCGNCGCGGGGQVAEDIEPGDLDVRQLPHGQRHEVIFTMLGRLE
                     PGQYFILANDHDPKPLRYQLEAQHPDTYFFDYLQSGPDVWRVRIGRVSAAQA"
     gene            1415..2107
                     /locus_tag="VLC50_01400"
     CDS             1415..2107
                     /locus_tag="VLC50_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016189305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HSF26149.1"
                     /translation="MADETLDSRRHHVLAGASRAAVLDLLRRTQRPMAVSEVAEAVGL
                     HANTVRTHLDLLTETGYAYRETEPPRGPGRPRVVYSATSTTDDGRNYRLLAELLARYL
                     VATNEHPGDAAVEAGRHWAKNVAIRVDAERPDGVSGPAAVARVVHLLADAGFSPELGD
                     DGMSIYLHSCPFRELAHSQRDVVCGAHLGIIQGALNELGAPVEAPTLLPFVEPDLCVA
                     TLREPSDATTRG"
     gene            2118..3320
                     /locus_tag="VLC50_01405"
     CDS             2118..3320
                     /locus_tag="VLC50_01405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF26150.1"
                     /translation="MDHTPSPAAPASAPRPPRSTATSVAPAKFAVRRVPLLLLGGASL
                     LMGLAGGLARIGSGMPSPQSAAVGHGVLMALGFLGTLITLERSVAMGRSWGFATPVLS
                     GLGAVLLVAGAPSPVPELVLTAAGAWLTAVYVVMWQMQQAPHIAVQLLGALAWWGAGV
                     RWLTGGSLSQIAPWLAGFLVLTIVGERLELSRFAIVGRTAGNTMLAASGVLSLGMLVG
                     LADADAGARVFGLALVLFGLWATRFDLIRRTIHTPGLTRYMAVCMAAGYAWLSVAGLL
                     WMVHGSVTDGPVYDAALHAVFLGFAMSMVFGHAPVILPAVLRIRLPHHGRDYVVLAIL
                     HASLLLRLGLGDGLGLTPARILGGILGAIALLAFVANSAFTAAAASRAPAPRRPVSTT
                     TQPERMPR"
     gene            3317..3877
                     /locus_tag="VLC50_01410"
     CDS             3317..3877
                     /locus_tag="VLC50_01410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF26151.1"
                     /translation="MTTSSPARRARVRKIRGKEVAPVEAAALSARAVLERELAALHAL
                     REAHAPLAELKAAHARTKRAVTLAYNGAAAAASTLSGSYLAWRAWRSEATRLSTLRQQ
                     LMMRERDDLGVLPANAVRIGTRAAIRPLQAGTHTEATSYIRATVGLPRIGVDLPATLA
                     TAQATDAARAGARAAQARPAAIAAAS"
     gene            3894..6644
                     /locus_tag="VLC50_01415"
     CDS             3894..6644
                     /locus_tag="VLC50_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011780818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HSF26152.1"
                     /translation="MPTAPVGLTLDRTGSAGAGGGGLDRAGRAHWHARANAVVLGYLA
                     LAGLALVLHAQLPMPRWLAVHLLLLGAVTNAIVTWSEHFAVALLRAPAPSRRRSAARL
                     AVLNLAVVGVLAGVSLEHAGLVAAASVLLGLVVAEHTGTLLVRAHRSLPGRFGRTVRF
                     YVAAGVALLVGATLGALVATGALGHETHEALHAAHVHANLLGWVGLTVLGTLFTLWPT
                     VLHTRMVDGVMRAAMWCLALTTAGLAVAVVGFVLDQRPVAIVGLAGYAAGLAMALRPF
                     VATWRRKRPYDLASWSLAAATGWLLAIVLADLVLLARSADLATYLERLDELVPPMLVG
                     FVGQVLLGALSYLLPVVLGRGPVGARAAARALARAWPARVGALNLGVVLIALPLPDPF
                     PRLGWALVLAALTGFVALAVPVLLAGRKPAPTPTSTAESVTVTHSPTTDSHPPVATPP
                     PAAAASRRRNPALIGLVIGAVMTLIPVGIALSGDAGSGGPATVVASEATQEVPVTLVN
                     MDIRPGVIEVAAGTRLRLVVTNQDAMRHDVAFPDGPKTRLLAKGQSQTLDLGVVTADL
                     SGWCTVPGHKAAGMTLDIKVTGGSAPAANGSAGTSGSMPGMNHGAHGNGSASASEPSV
                     TTVDVHGKPGPGWTPYDATLAPAPGGTVHKITVRASEKDMEVAPGVRQRMWTFNGTVP
                     APTLRGQVGDVFEVTLVNDASMGHGIDFHAGSLAPDVPMRTIDPGESLVYRFRADFAG
                     AWLYHCSTMPMLAHIGNGMYGAIIIDPPGLPKVDREYVLVSAEQYLGPQGGIADMAKL
                     RANAWDTAVFNGYPDQYVHAPLTARVGERVRFWVVAAGPIEGTALHIVGAQFDTVYKE
                     GAYLLRAGNADRGAAQVLDLAPAQGGFVETVFPEAGHYPLMDHDMRRGENGAHGVVEV
                     TP"
     gene            6644..7084
                     /locus_tag="VLC50_01420"
     CDS             6644..7084
                     /locus_tag="VLC50_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011293218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF26153.1"
                     /translation="MLGTWSLVRFLHVVSAMIWVGGQLTITAMVLPVVRRKLAGPAML
                     PVMTGVGRRFGKFTVTVFLPLQVGTGVLLAWHKGVTWSSLSDPGYGRTLAAKLVAFAL
                     VMLAAGGHGWARGSGHPVLARSLAIGSLIGSLAIVLLATALPVS"
     gene            7189..9048
                     /locus_tag="VLC50_01425"
     CDS             7189..9048
                     /locus_tag="VLC50_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020549296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HSF26154.1"
                     /translation="MPKQTQQLDRVVIRFAGDSGDGMQLTGDRFTAETALLGNDLSTL
                     PNFPAEIRAPAGTLPGVSSFQLHFADHDVLTPGDAPDVLVAMNPAALKANLRDLARGG
                     SLLVNVDEFTPRALAKVGYDANPLEDGSLDGYHVHEVPLTSMTVRALEPFKLGKKAAE
                     RAKNMFALGLLSWLYSRPTEATEAFLRRKFAGDQAILEANLAALRAGWAFGETTEDFS
                     ISYEVKPAPTPPGTYRNITGNLALAYGLVAAARSVGLPLFLGSYPITPASDVLHTLSK
                     LKSFGVVTFQAEDEIAGVGAALGASYAGALGVTTTSGPGVALKSETIGLAVALELPLV
                     ICDIQRGGPSTGLPTKTEQADLLQAMFGRNGESPVPIVAPRSPGDCFDAAIEAARIAT
                     TYRTPVFLLSDGYLANGSEPWRIPAVDDLPAHPVAFATEPNGPDGTFLPYLRDPETLA
                     RPWAVPGTPGLEHRIGGIEKADRTGNISYDPANHDHMVRMRQGKVDAIAATVPPLTVD
                     DPSEQARVLVIGWGSTYGPVAAACRTLRGEGHAVAHAHLRHLNPFPANTGDVLRGYDR
                     VVVPEMNLGQLALLLRARYLIDIAGFNLVRGQPFTSAEIADVLRTTVKEVAGG"
     gene            9045..10136
                     /locus_tag="VLC50_01430"
     CDS             9045..10136
                     /locus_tag="VLC50_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018654778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HSF26155.1"
                     /translation="MSTGLTSATDQQPSDVSGVGGVPRLDGDTTLTKKDFTSDQEVRW
                     CPGCGDYAILAAVQSFLPELGLRRENIVFVSGIGCSSRFPYYLDTYGMHSIHGRAPAI
                     ATGIAVTRPDLAVWVVTGDGDALSIGGNHLAHALRRNLNITILLFNNRIYGLTKGQYS
                     PTSEQGKITKSTPMGSLDTPFNPVSFALGAEATFVGRALDSDRKSLTAVLRAAASHRG
                     ASLVEIYQNCNIFNDGAFEALKDPATRDEMLIHLEHGQPIRWGASGEHGLVRDPVTGA
                     FAVAEVAEVGEGAIVVHDAHTDDPAYAFALSRLTDGAIGRTPVGIFREVTRPSYDDLA
                     RAQVREAQAGAGAGDLATLLAGGDTWTVH"
     gene            10166..10567
                     /locus_tag="VLC50_01435"
     CDS             10166..10567
                     /locus_tag="VLC50_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007074143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HSF26156.1"
                     /translation="MELDDDPARLDVDLVHGWLSAQAYWALGRTRATVARSLAASTVL
                     GAYEDGQLVGLARAVTDGATFAWVCDVFVERAHRGRGIGSALVRELVTRLRDGGVYYV
                     LLATQDAHEVYRRLGFTELAEPSRWMQLDLR"
     gene            10629..11528
                     /gene="rarD"
                     /locus_tag="VLC50_01440"
     CDS             10629..11528
                     /gene="rarD"
                     /locus_tag="VLC50_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010475928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HSF26157.1"
                     /translation="MDQTRRGTIAGVMAYLIWGAFPLYWPLLEPAGAVEILAHRIVWS
                     LAFLVALLALARNWSWLRSMHGARLAWLSVAAVTIAVNWGVYIWAINSGHVVETSLGY
                     FINPLVTVALAVVLLGERLRRVQWAALGLALVAVAVLTAGYGRPPWIAFTLALSFGTY
                     GLAKKKANVPAVESLTVETALLFLPGLAYLGWLQSRDQLVFAHWSAANTALLMLCGVV
                     TAVPLLCFGYSAIRVRLSTLGLLQYLTPGLQFLLGVLVFREHMPLTRWVGFALVWAAL
                     AVFTLDTLGGRRRREQAVASAAA"
     gene            11761..12567
                     /locus_tag="VLC50_01445"
     CDS             11761..12567
                     /locus_tag="VLC50_01445"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013677089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSF26158.1"
                     /translation="MGATAKLTVSDLQLGYRSKTTGRFRWACRDLSLEVANNEFVAIV
                     GPSGCGKTTFLSAVAGLLPVTAGTLALNGAQIRGPGTDRSLVFQHASLFPWRTVTANI
                     EFGLEAQGRLDAAGRARAEELLRLVGLEGEGGKHPRELSGGMSQRVNLARALATDPDL
                     LLLDEPFAALDAQTRGVMQEELTRIWQVSGVSGGKTAIFITHDVPEAVFLADRVIVFS
                     QGPAHVLECVSIDLPRPRAPELKRSPELQSYAEHILELIMTAPQQRVAQR"
     gene            12648..13391
                     /locus_tag="VLC50_01450"
     CDS             12648..13391
                     /locus_tag="VLC50_01450"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSF26159.1"
                     /translation="MLLGLCGVIGVLAIWQLATSLGAIDSRFSSSPLGAFQGLVSLAG
                     SGEIWGSTANTLIAVAEGIGITLAVGIPVGLITGRVSVLYGLFEPLISVMYAVPFVIF
                     LPIIIFWFGIGDKARLVIIIWASIFPLLINVVAGARNLDRNYLQVSQVFCASRKKTLF
                     SVALPGTMPYILAGIRQSVGRALVGAIVAELFIGSHGIGFLVQLETSNFEMDKAMGAI
                     AVIAAVAILLTRGVAFLEQRLTFWSDSSD"
     gene            13418..14464
                     /locus_tag="VLC50_01455"
     CDS             13418..14464
                     /locus_tag="VLC50_01455"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HSF26160.1"
                     /translation="MRRKTLISGIGVVIALSLTACGNSPTATSSEATTGSSGQTSAAS
                     APAKVTKMTVGYPSPAAGYTDLYICVDEGIYAKNNLDVELVQLKSSSQLLSALSSGAV
                     QIGSGVAASTAAGALKGVDLKYIGIPLPVLYLEMWGDADITSPEQLKGKKVAVTPPGS
                     QSSLAMEKLLTDLNMKDDVELVHVADVAAEVAALENGSVNALVTQPPTGTKTRDKGFH
                     KIMDFTNYPVAANAFAVMTDYIDKNKEAVAAFVKSEVECLALLHKDKAKGVASITKHL
                     NLTDASLAEYSYDYFEKIWAKVPTVQPDLIKASFEEAASKAKVDAPADITKYVENSFI
                     EELEKSGFIDSLYK"
     gene            14591..16078
                     /locus_tag="VLC50_01460"
     CDS             14591..16078
                     /locus_tag="VLC50_01460"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HSF26161.1"
                     /translation="MTEDFDLVEASIAGIHEAYLCRALSCRELVEGYLERIQAYDAAG
                     PRLNCIITVNPDVRAQAAELDAALATGDLAGPLHGIPIVVKDQIDVAGMPTTMGSTLF
                     ADYVPGRDSSVVSRLKAAGALILAKTTLGELGAGDTHGSLFGSTRNPYRLEHTPGGSS
                     GGSAVAVAANLTTAALGQEGLASITRPSAWNSIVGMRPTAGLVSRAGAYSGWPNRASS
                     IGPMARTVEDAARLLDVIAGYDPDDPLTATGVGHLSGPTVGQLCPKALDGARVGVLRQ
                     SIGLGSEPDSEDFTRVSVVFDRAIADLRAAGAIVVDPVEVPRLQELLDQRAFEHAEAS
                     FEAWMARSEHPPYRSHAELAAQPAYQDLVHRRSGGHLRRFGSSSHYAYLRARDELRIN
                     LLKVMADARLDVLVLKSVEHTATPISEGTAPPFTDGKGAIHLATFLIDVPSITVPAGA
                     TPDGLPVGLSFLGRPFTDAAVLGYAYAYEQHTRRRRPPEGFPPLR"
     gene            complement(16095..16886)
                     /locus_tag="VLC50_01465"
     CDS             complement(16095..16886)
                     /locus_tag="VLC50_01465"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HSF26162.1"
                     /translation="MTTLSRKGLTPAATARLARFGTIAMEGHIVPDAAATWGTLTEAA
                     YRRIRIMLLHQELRPGERTSVAHLAAATGLGRAPVKSAIERLAVEGLLQVRGRSGTRV
                     ARLDDDDITNLFEMRRIYETAAAPLIAERMTDEQFQELAEAASTLTSSKSDGNDGESV
                     ADMVGFIDADVKFHRLSMACANNPYLLAHYASLNLHLLISHYLLIDRGFHAAERLREH
                     EKIVAAIKDRDAEAIAAAMRAHADAVEAGIHSTMHQYGTLSVHGA"
     gene            complement(16840..>17768)
                     /locus_tag="VLC50_01470"
     CDS             complement(16840..>17768)
                     /locus_tag="VLC50_01470"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HSF26163.1"
                     /translation="PMRLAVPDLVSSSYFPVLAAAELGLFAQEGVDVELQQVYPVTSA
                     AIALRDGEIDFLVGPAHAPLKAFPDWAGVKLLAAVAQHIYWLLVVRCDLDVAPGDLAG
                     LHDLRIAAARGPEQALRQLFAEAHVSLDDNRITIGPPPASEGQDTSFGLSAAAALRDG
                     RIDAFWANAMAAEIAVNDGAGRVFLDPRRGQGPARARSFTFAAAMATDAVIAEHPRQV
                     AALVRGIVGAQQALKEDPQRAAAVGRRLFPAAEAGFITDLVRRDLPFYDAKISHEAVD
                     GLNGFARRAGMCSAEVSYDDVVAQGLDTRGDC"
BASE COUNT         2473 a         6570 c         6046 g         2679 t
ORIGIN      
        1 gcgcgccgcc gagcgggagc tggcccgcgt cgcgcgcacc ggctcgcggc tggcggtggc
       61 cgtcatcgac ctcgacggct tcaagaaggt caacgacacc ctggggcacg cggccggcga
      121 ccagctgctc gtcacgatcg cgacggcgtg gtcgaaggtg ctgcgcccga ccgacgtgct
      181 cgcccggcac ggcggcgacg agttcgtgct gctgctgccc ggcgccggcg cggccgaggc
      241 gaccacggtg ctgcaccgcc tgctgcgcgc caacgcggac atcggctggt cctacgggct
      301 caccgcctac gagcccggcg acacgctgga ccggctgctg gcccgggccg acgtccgcct
      361 ctatgacgcc aaggccgctc gcacgccgcc gacgagctca ccgctgtgtg cgcaggtctc
      421 cccgggggcg atggcggtct gagcacagat gccgtacggc ggccgcagct gcgaccgccg
      481 tacggcgtct tgggtgtcga cctggcctga aggccgaggt caggcctgag cggccgacac
      541 ccggccgatg cgaacccgcc acacgtcggg cccggactgc aggtagtcga agaagtaggt
      601 gtcggggtgc tgtgcctcga gctggtagcg cagcggcttg gggtcgtggt cgttggcgag
      661 gatgaagtac tgccccggct cgagccggcc cagcatcgtg aagatcacct cgtgccgctg
      721 accgtgcggg agctggcgca cgtcgaggtc gccgggctcg atgtcctcgg ccacctggcc
      781 gccgccgccg cacccgcagt tgccgcaccc acacccgccg tggacctcct cggcgtgccc
      841 gccgagcagg tcgtgcatgc cctcgagcag cgccgcgatg tcgacaccgc ccgcgacgag
      901 cgccgggagc agcaggtcgt tctccttggc caggtgcgcc tcgaagacgg cgtgcaccgc
      961 ggcggccgcg gcgaccgtct cggccggggt gctggccgcg gcgagcgcgt cggtgagctt
     1021 catgatcacc cggtgctcgg cgatcatccc cttgacgagc aggccggatg ccggcaggtg
     1081 ctcgccggcg ccgtagaggg tggcctcctc ggcggccgcg tgcggaagga cctcggcggt
     1141 gatgaagccg agcaggtcgt cgcgggcgcc ggtgaccggg gccaaccgct cgaccgcctc
     1201 gcggacccgg tcgaccccgt gcgcgaagtc gtcggacagc tgcgcgtggt gggacttgat
     1261 ggccgcgatg gtctctgcgg cgccaggggg cagctcggcg acgtccgcgc cgatggcagg
     1321 tgctgtggtc atgggcggtg cgctcctcgg ggtgggtccg tagttttcac ggctagggtt
     1381 gtttttacta tggggtccgt ggaaaatgca aggcatggcg gatgagactc tcgactcacg
     1441 caggcaccac gtgctggccg gggccagccg agccgccgtg ctcgacctgc tgcggcggac
     1501 ccagcgaccg atggcggtct ccgaggtcgc cgaggcggtc ggcctgcatg ccaacaccgt
     1561 acgcactcac ctcgacctgc tcaccgagac cggctacgcc taccgcgaga ccgagccgcc
     1621 ccggggaccc ggccgtcccc gcgtcgtcta cagcgcgacc tcgaccaccg atgacgggcg
     1681 caactaccgg ctgctcgccg agctgctggc ccgttacctg gtggcgacca acgagcaccc
     1741 cggcgacgcc gcggtcgagg ccggccggca ctgggccaag aacgtcgcca tccgggtcga
     1801 cgccgagcgc cccgacgggg tcagcggccc tgcggcggtg gcccgggtcg tgcacctgct
     1861 cgccgacgcc gggttctccc ccgagctggg cgacgacggc atgagcatct acctgcacag
     1921 ctgcccgttc cgcgagctcg cccacagcca gcgcgacgtc gtctgcggag cccacctggg
     1981 aatcatccag ggggcgctga acgagctcgg ggcccccgtc gaggccccca ccctgctgcc
     2041 cttcgtcgag cccgacctgt gcgtggccac gctgcgcgag ccctccgacg cgacgacgcg
     2101 aggctgaccc accaccgatg gaccacactc cctcccccgc cgcgccggca tcggccccgc
     2161 ggcctccccg cagcacggcc acctcggtgg cgccggccaa gttcgccgta cggcgggtgc
     2221 cactgctcct gctcggcggg gccagcctgc tgatgggcct ggccggcggg ctggcccgga
     2281 tcggctccgg gatgccctcg ccgcagagcg ccgcggtcgg ccacggcgtc ctcatggccc
     2341 tcggcttcct cgggacgctg atcacgctcg agcgctcggt cgcgatgggc cggtcctggg
     2401 ggttcgccac cccggtgctg tccgggctcg gcgcggtgct gctcgtcgcc ggtgccccca
     2461 gcccggtgcc cgagctcgtg ctgacggcgg ccggagcctg gctgaccgcg gtctatgtcg
     2521 tgatgtggca gatgcagcag gctccgcaca tcgccgtaca gctgctcggt gccctggcct
     2581 ggtggggcgc cggggtgcgc tggctgaccg gcgggtcgct gtcgcagatc gcgccatggc
     2641 tggccggctt cctcgtgctc accatcgtcg gcgagcgcct cgagctctcg cgcttcgcca
     2701 tcgtcgggcg cactgcgggc aacaccatgc tcgccgcctc cggcgtgctg tcgctgggga
     2761 tgctcgtcgg cctggccgac gccgacgccg gcgcccgggt cttcgggttg gcgctggtgc
     2821 tgttcgggct gtgggccacc cgcttcgacc tcatccggcg caccatccac acgcccgggc
     2881 tgacccgcta catggcggtg tgcatggccg ccggctacgc ctggctgtcc gtggcggggc
     2941 tgctgtggat ggttcatggg tcggtcaccg acgggccggt ctacgacgcc gcgctgcacg
     3001 cggtgttcct cggtttcgcg atgtcgatgg tcttcggtca tgcgccggtg atcctgccgg
     3061 cggtgctgcg gatccggctg ccccatcacg gccgcgacta tgtcgtgctc gcgatcctgc
     3121 acgcctcgct gctgctgcgc ctcgggctcg gcgacggcct gggcctgacg cccgcccgga
     3181 tcctcggtgg gatcctcggc gccatcgcgc tgctcgcgtt cgtcgccaac tccgccttca
     3241 ccgccgccgc ggccagccgc gccccggcac cccgccgacc cgtctccacc acgacccagc
     3301 ccgagaggat gccccgatga ccaccagctc acccgcccgc cgcgcccgcg tgcgcaagat
     3361 ccgcggcaag gaggtggccc cggtcgaggc cgccgccctc tcggcgcggg cggtgctcga
     3421 gcgtgagctc gccgcgctgc acgccctgcg tgaggcccac gccccgctgg ccgagctcaa
     3481 ggccgcgcac gcacggacca agcgggcggt gacgctggcc tacaacggtg ccgcggccgc
     3541 cgcctcgacg ctgtccggga gctacctggc ctggcgggcc tggcgcagcg aggcgacccg
     3601 gctgtccacc ctgcgccagc agctgatgat gcgtgagcgc gacgacctcg gcgtgctgcc
     3661 ggccaacgcg gtgcgcatcg gcacccgggc tgcgatccgc ccgctgcagg ccggcacgca
     3721 caccgaggcg acctcctaca tccgggccac ggtgggactg ccgcggatcg gggtcgacct
     3781 gccggccacg ctggcgacgg cccaggccac cgacgccgcc cgcgccggcg cccgcgcggc
     3841 gcaggcccgc cccgcggcga tcgcggcggc ctcctgaccg ccctagcacc accgtgccca
     3901 ccgctcccgt cggcctgacc ctggaccgca ccggctccgc cggcgcgggg ggcggcgggc
     3961 tcgaccgcgc cgggcgggcg cactggcatg cccgcgccaa cgccgtggtg ctcggctacc
     4021 tggcgctggc cgggctcgcg ctcgtcctgc acgcgcagct gccgatgcct cgctggctcg
     4081 cggtgcacct gctgctgctc ggcgcggtga ccaacgcgat cgtcacgtgg agcgagcact
     4141 tcgcggtggc gctgctgcgg gccccggcgc cgagccgccg ccgctcggcg gcgcggctgg
     4201 cggtgctcaa cctcgccgtc gtcggcgtgc tcgccggggt cagcctggag cacgccggcc
     4261 tggtcgcggc cgccagcgtc ctgctcgggc tcgtcgtcgc cgagcacacc ggcacgctgc
     4321 tggtgcgggc ccaccgctcg ctgcccgggc gcttcgggcg caccgtgcgc ttctacgtcg
     4381 cggccggggt cgccctgctc gtgggtgcga cgttgggcgc cctcgtcgcc accggcgcgc
     4441 tcgggcacga gacgcacgag gcgctgcacg cggctcacgt gcacgccaac ctcctgggct
     4501 gggtcgggct caccgtgctc ggcacgctgt tcacgctctg gcccaccgtg ctgcacaccc
     4561 gcatggtcga cggcgtcatg cgcgccgcca tgtggtgcct ggccctgacc accgccgggc
     4621 tggccgtggc cgtggtcggc ttcgtccttg accagcgccc ggtcgcgatc gtcgggctgg
     4681 ccggctacgc cgcgggtctc gccatggcgc tgcggccctt cgtcgccacc tggcgccgca
     4741 agcggcccta cgacctggcc tcctggtcgt tggcggcggc caccgggtgg ctgctggcga
     4801 tcgtgctggc cgacctggtt ctgctggccc gctccgcgga cctggccacc tatctcgagc
     4861 gcctcgacga gctggtcccg ccgatgctgg tcggcttcgt cgggcaggtg ctgctcggcg
     4921 cgctgtccta cctcctgccg gtcgtgctcg ggcgggggcc ggtgggggca cgcgccgcgg
     4981 cgcgcgcact cgcgcgcgcc tggccggcgc gcgtcggggc gctcaacctc ggcgtcgtgc
     5041 tgatcgccct tccgctgcct gacccgtttc ctcggctcgg ctgggcgctg gtgctggccg
     5101 cactgaccgg gttcgtcgcc ctggccgtcc ccgtgctgct ggccggccgc aagcccgctc
     5161 ccacccccac ctcaaccgca gagagcgtca ccgtgaccca cagccccacc accgactccc
     5221 acccacccgt ggccaccccg ccgccggccg cggcagcctc acgccggcgc aaccccgcgc
     5281 tgatcggcct ggtgatcggg gccgtgatga cgttgatccc ggtcgggatc gcgctctccg
     5341 gcgacgccgg ctccggcggg ccagccaccg tcgtcgcctc cgaggccacc caggaggtgc
     5401 cggtcaccct ggtcaacatg gacatccggc ccggggtgat cgaggtcgcc gcgggcaccc
     5461 ggctgcggct ggtcgtgacc aaccaggacg ccatgcgcca cgacgtcgcc ttccccgacg
     5521 gccccaagac ccggctgctg gccaaggggc agagccagac cctcgacctg ggcgtcgtca
     5581 ccgccgacct gtccggctgg tgcaccgtgc ccgggcacaa ggccgccggc atgaccctcg
     5641 acatcaaggt caccggcggg tcagcacccg cggctaacgg ttctgcgggg acgtccgggt
     5701 cgatgcccgg catgaaccac ggcgcccatg gcaatggcag cgcctcggcc agcgagccgt
     5761 cggtcaccac cgtggacgtc catggcaagc cggggcccgg ctggacgccg tacgacgcga
     5821 ccctggcacc ggcgccgggc ggcaccgtgc acaagatcac cgtgcgagcc tccgagaagg
     5881 acatggaggt cgcacccggg gtccggcagc ggatgtggac cttcaacggc accgtgccgg
     5941 ccccgacgct gcgagggcag gtcggcgacg tgttcgaggt gacgctggtc aacgacgcct
     6001 cgatgggcca cggcatcgac ttccacgccg gctcgctggc ccccgacgtg ccgatgcgca
     6061 cgatcgaccc cggcgagtcc ctggtctacc ggttccgggc cgacttcgcc ggtgcctggc
     6121 tctaccactg cagcacgatg ccgatgctgg cgcacatcgg caacggcatg tacggcgcga
     6181 tcatcatcga cccgccgggg ctgcccaagg tcgaccgcga atacgtcctg gtctcggccg
     6241 agcagtacct cggcccccag ggtgggatcg ccgacatggc caagctgcgg gccaacgcct
     6301 gggacaccgc ggtcttcaac ggctaccccg accaatatgt ccacgccccg ctgaccgccc
     6361 gggtgggcga gcgggtgcgg ttctgggtgg tcgccgccgg cccgatcgag ggcaccgcgc
     6421 tgcacatcgt cggcgcccag ttcgacaccg tctacaagga gggcgcctac ctgctgcggg
     6481 ccggcaacgc cgaccgcggc gccgcccagg tgctcgacct ggccccggcg cagggcggct
     6541 tcgtcgagac ggtcttcccg gaggccgggc actacccgct catggaccac gacatgcgcc
     6601 gcggcgagaa cggcgcccac ggcgtcgtgg aggtcacccc ctgatgctgg gcacctggtc
     6661 gctggtgcgg ttcctgcacg tggtctcggc gatgatctgg gtcggaggcc agctgacgat
     6721 cacggcgatg gtgctgcccg tcgtacggcg caagcttgcc ggcccggcga tgctgccggt
     6781 gatgaccggc gtgggccggc gcttcggcaa gttcaccgtc accgtcttcc tgccgctgca
     6841 ggtcggcacc ggggtgctgc tggcctggca caagggagtc acctggtcct cgctgtccga
     6901 tcccggctac ggacgcacgc tggccgccaa gctcgtcgcg ttcgccctgg tgatgctcgc
     6961 cgcgggcggg cacggctggg cccggggcag cggtcacccg gtgctggccc ggtcgctggc
     7021 gatcggctcg ttgatcggct cgctggccat cgtgctgctc gccaccgcgc tgcccgtctc
     7081 atgaacccga ccggccgggc cggcccagcc tctcggacgt agcatgggaa agcacacgta
     7141 ggcccgcatc ccgctcgccc cgtgccgccg aacgcgaaga aggacgtcgt gcccaagcag
     7201 acccagcagc tcgaccgggt ggtcatccgt ttcgcgggcg actccggcga tggcatgcag
     7261 ctgaccggcg accgtttcac cgctgagacc gcgttgctgg gcaacgactt gtcgacgctg
     7321 cccaacttcc cggccgagat ccgcgcgcca gccggcacgc tgcccggggt gtcgagcttt
     7381 cagctgcact tcgccgacca tgacgtgctc acgccgggcg acgcccccga cgtgctggtg
     7441 gcgatgaacc ccgccgcgct gaaggccaac ctccgtgacc tggcccgggg cgggtcgctg
     7501 ctggtcaacg tcgatgagtt caccccgcgg gcgctggcca aggtgggcta tgacgccaac
     7561 ccgctcgagg acggctcgct cgacggctac cacgtccacg aggtgccgct cacgtcgatg
     7621 acggtgcgcg ccctcgagcc cttcaagctg ggcaagaagg ccgccgagcg ggccaagaac
     7681 atgttcgcgc tcgggctgct gtcctggctc tactcgcgcc cgaccgaggc gaccgaggcg
     7741 ttcctgcggc gcaagttcgc cggcgaccag gcgatcctcg aggccaacct ggcggcgctg
     7801 cgggccggtt gggcgttcgg ggagacgacc gaggacttct cgatctccta cgaggtcaag
     7861 ccggccccca ccccgccggg cacctaccgc aacatcaccg gcaacctcgc gctggcctac
     7921 ggcctggtcg cggccgcccg cagtgtcggg ctgccgctct tcctcggctc ctatccgatc
     7981 actccggcca gcgacgtcct gcacacgctg tccaagctca agagctttgg cgtcgtgacc
     8041 ttccaggcag aggacgagat cgccggcgtc ggcgcggcgc tcggggcgtc gtacgccggt
     8101 gctctcggtg tcaccacgac gtcgggcccc ggggtggcgc tcaagtcgga gacgatcggg
     8161 ctggccgtgg cgctggagct gccactcgtg atctgcgaca tccagcgcgg cgggccctcg
     8221 accgggctgc ccaccaagac cgagcaggcc gacctgctcc aggcgatgtt cggacgcaac
     8281 ggcgagtcgc cggtgccgat cgtggccccg cgctcgcccg gtgactgctt cgacgcggcg
     8341 atcgaggcgg cccgcatcgc caccacctac cgcacgccgg tcttcctgct ctccgacggc
     8401 tacctagcca acggctccga accgtggcgg atccccgcgg tcgacgacct gcccgcgcac
     8461 ccggtggcgt tcgcgaccga gcccaacgga cccgacggca cgttcctgcc ctacctgcgc
     8521 gaccccgaga cgctggcccg gccctgggcg gtgccgggca ccccgggtct cgagcaccgc
     8581 atcggcggca tcgagaaggc cgaccgcacc ggcaacatct cctacgaccc ggccaaccac
     8641 gaccacatgg tccggatgcg ccagggcaag gtcgacgcga tcgccgcgac ggtgccgccg
     8701 ttgacggtcg acgacccgtc ggagcaggcg cgggtgctgg tgatcggctg gggctcgacc
     8761 tacggcccgg tcgcggcggc ctgccgcacc ctgcgcggcg agggccacgc ggtggcccac
     8821 gcccacctgc gccacctcaa cccgttcccc gccaacaccg gcgacgtgct gcgcggctac
     8881 gaccgggtgg tcgtccccga gatgaacctg gggcagctgg ccctgctgct gcgcgcgagg
     8941 tacctcatcg acatcgccgg cttcaacctg gtgcggggac agccgttcac ctccgccgag
     9001 atcgccgacg tgctgcgcac caccgtgaag gaggtggccg gcggatgagc accggcctga
     9061 ccagcgccac cgaccagcaa ccgagcgacg tgtccggcgt cggcggtgtg ccccgcctcg
     9121 acggggacac cacgctgacc aagaaggact tcacctccga ccaggaggtg cgctggtgcc
     9181 ccggctgcgg cgactacgcg atcctcgccg cggtccagtc gttcctgccc gagctcgggc
     9241 tgcgccggga gaacatcgtg ttcgtctccg gcatcggctg ctcctcgcgg ttcccctact
     9301 acctcgacac ctacgggatg cactcgatcc acgggcgggc cccggcgatc gccaccggca
     9361 tcgcggtcac ccgcccggat ctggccgtct gggtcgtcac cggtgacggc gacgcgctct
     9421 ccatcggcgg caaccacctg gcccacgcgc tgcggcgcaa cctcaacatc acgatcctgc
     9481 tgttcaacaa ccggatctac gggctcacca agggccagta ctcccccaca tccgagcagg
     9541 gcaagatcac caagtcgacg ccgatgggat cgctggacac gccgttcaac ccggtctcgt
     9601 tcgccctggg cgccgaggcg acgttcgtcg ggcgagccct cgactccgac cgcaagagcc
     9661 tgaccgcggt gctgcgcgcg gccgcctccc accgcggcgc ctcgctggtc gagatctacc
     9721 agaactgcaa catcttcaac gacggcgcat tcgaggcgct caaggacccg gccacccgcg
     9781 acgagatgct catccacctc gagcacggcc agccgatccg ctggggcgct tccggcgagc
     9841 acgggctcgt ccgcgacccg gtcaccggtg cgttcgcggt ggcggaggtg gccgaggtcg
     9901 gcgagggcgc gatcgtcgtg cacgacgccc acaccgacga cccggcatac gcgttcgcgc
     9961 tgtctcgact aaccgacggg gcgatcgggc gcaccccggt cggcatcttc cgcgaggtca
    10021 cccgacccag ctacgacgac ctggcccgcg cccaggtgcg cgaggcccag gcgggcgctg
    10081 gtgcgggcga cctcgccacg ctgctcgccg gcggcgacac ctggaccgtg cactgagcac
    10141 cgccaggctt ccggtccccg agggcatcga gctcgacgac gaccctgctc gtctcgacgt
    10201 cgacctcgtg cacgggtggc tgtcggcgca ggcctactgg gccctcggac gcacccgcgc
    10261 gacggtggcg cgatccctgg ccgcctcgac ggtgctcggt gcctacgagg acgggcagct
    10321 ggtcgggcta gcccgggcgg tgaccgacgg cgccacgttc gcctgggtct gcgacgtgtt
    10381 cgtcgagcgc gctcatcgcg gccggggaat cggatcggcg ctggtccgcg agctggtcac
    10441 gcggctgcgc gacggtgggg tctactacgt gctgctggcc acccaggacg cccacgaggt
    10501 ctatcgccgg ctcggcttca ccgagctcgc cgaaccctcc cgctggatgc agctcgacct
    10561 ccgctgaccc ccgccctggt ccgccgaccg ttggtgaggc gtgaacggcc gaccggtacg
    10621 gtcgcgaggt ggaccagacc agacgcggca ccatcgcggg ggtgatggcc tacctgatct
    10681 ggggcgcctt cccgctctac tggccactgc tcgagcctgc gggcgcggtc gagatcctcg
    10741 cgcaccggat cgtctggtcg ctcgccttcc tcgtcgcgct gctcgccctg gcccgcaact
    10801 ggagctggct gcgcagcatg cacggggccc ggctggcctg gctgtcagtg gccgccgtga
    10861 cgatcgccgt caactggggc gtctacatct gggcgatcaa ctccggacac gtggtcgaga
    10921 cctcgctcgg ctacttcatc aacccgctgg tcacggtggc gctggccgtg gtgctgcttg
    10981 gcgaacgact gcgccgggtg cagtgggccg ccctcggact ggcgctggtg gccgtcgcgg
    11041 tgctgaccgc aggctacggc cgcccgccgt ggatcgcgtt cacgctcgcg ctgagcttcg
    11101 gcacctacgg cctggccaag aagaaggcca acgtcccggc cgtggagagc ctcacggtcg
    11161 agaccgcgct gctcttcctg ccgggcctgg cctacctcgg ctggctgcag agtcgcgacc
    11221 agctggtctt cgcgcactgg tcagccgcca acaccgcgct gctcatgctg tgcggggtcg
    11281 tcacggcggt gccgttgctg tgcttcggct acagcgccat ccgggtgcgg ctgtccaccc
    11341 tcgggctgct gcagtacctc accccggggc tgcagttcct cctcggcgta ctcgtgtttc
    11401 gcgagcacat gccgctcacc cgctgggtcg ggttcgcctt ggtctgggcg gcgctcgcgg
    11461 tgttcacgct ggacacgctc ggcggccgcc gtcgtcgcga gcaagcggtg gcctccgccg
    11521 cggcgtgagc ccttcgggcg aatagccgct cgtcgtcctc ttcatccaac ctcttgaccc
    11581 gccgagaaca actgacctac tgttctggca tgccagattg acggctgaat ccggcatgag
    11641 agctgccggc cgcaggccgg tccgacagga gcgcatgccg ctgaccaggc tcatgacgtc
    11701 acagcagctg cactcgaccg gtgaaacgcg caattcacat caacgagagg agccggtctg
    11761 atgggtgcaa cggccaagtt gactgtctct gatctccagc tcggatatcg ctccaagacc
    11821 accggtcgat tccgatgggc ctgccgcgac ctcagcctcg aggtcgccaa caacgagttc
    11881 gtcgcgatcg tcggcccgag cgggtgcggc aagacgacat tcttgtccgc cgtcgccggc
    11941 ctgctgccgg tcaccgcggg caccctcgcg ctcaacggcg cgcagatccg cggcccgggc
    12001 accgaccggt ccctcgtgtt ccagcacgcc tccctgtttc cctggcggac cgtcacggcc
    12061 aacatcgagt tcggcctgga ggcccagggt cgtctcgacg ccgccggccg agctcgcgcc
    12121 gaagagctcc tgcggctggt cgggctcgag ggcgaaggtg gcaagcaccc gcgtgagctg
    12181 tcgggaggca tgagccagcg cgtcaatctc gctcgcgccc tggccaccga cccggacctc
    12241 ctgctcctcg acgagccgtt cgctgcgctg gacgcgcaga cccggggcgt catgcaggag
    12301 gagctgacca ggatctggca ggtgagcggg gtgtctggcg gcaagaccgc aatcttcatc
    12361 acccacgacg tgcctgaggc agtgttcctc gccgaccgag tgatcgtctt ctcccaggga
    12421 ccggcgcacg tcctcgaatg cgtctcgatc gacctgcccc ggccccgggc gccagagctc
    12481 aagcgctcgc ctgagctgca gagctatgcc gagcacatcc tggagctcat catgactgcg
    12541 ccccagcaga gggtggccca acgatgacag ccacagactc ccagcagcgg acagacacgg
    12601 cctcgtcctt tgacgcccgg ccaccgtggt ggcgccgtca caaggaggtg ttgctcggtc
    12661 tctgcggcgt gatcggcgtc ctggcgatct ggcagctggc gacctccctg ggggcgatcg
    12721 actcgcggtt cagcagctcg cccctcgggg cgttccaggg gctcgtctcg ctcgccggct
    12781 caggtgagat ctggggctcg acggccaaca ccctgatcgc ggtggcggag ggcatcggca
    12841 tcacccttgc ggtcggcatc ccggtggggc tcatcaccgg ccgggtcagc gtgctctatg
    12901 gcctcttcga gcccctgatc agcgtgatgt acgccgtgcc attcgtcatc ttcctgccga
    12961 tcatcatctt ctggttcggc atcggggaca aggctcggct cgtcatcatc atctgggcct
    13021 cgatatttcc gctcctcatc aacgtggtgg ccggagctcg caacctggac cgcaactatc
    13081 tgcaggtgtc ccaggtcttc tgcgcctcgc gcaagaagac gctgttctcc gtcgcgctcc
    13141 ccggcacgat gccctacatc ctcgccggca tccgccagtc ggtggggcgc gctctcgtcg
    13201 gtgccattgt tgccgagctg ttcatcggct cgcacggcat cggtttcctg gtgcagcttg
    13261 agacgtccaa ctttgagatg gacaaggcga tgggggcgat cgcggtcatc gcggccgtgg
    13321 cgatcctgtt gacgcggggc gtcgcgttcc tcgagcagcg gttgacgttc tggtctgaca
    13381 gcagcgacta accccaaccc ccagacagga gcctcacatg cgcagaaaga cactgatcag
    13441 cggtatcggt gtggtgatcg cactctcctt gaccgcttgt ggcaactccc cgacggcgac
    13501 gtcgtccgag gcgacaacgg gctcgagtgg tcagacgagc gcggcatccg ccccggcgaa
    13561 ggtcacgaag atgaccgtgg gctacccctc gccggccgcc ggctacaccg acctctacat
    13621 ctgcgtggac gaggggatct acgccaagaa caacctcgac gtcgagctcg tccagctcaa
    13681 gtcgagctcg cagctgctct ccgcgctgtc cagcggcgcg gtccagatcg gttccggcgt
    13741 cgccgcctcg accgcagccg gtgccctcaa gggggtcgac ctcaagtaca tcggcatccc
    13801 actgcccgtc ctctacctgg agatgtgggg cgacgctgac atcacgtcac cggagcagct
    13861 caagggcaag aaggtggccg tcacgcctcc gggctcgcaa agcagccttg ccatggagaa
    13921 gctgctcacc gatctcaaca tgaaggatga cgtcgagctg gtccacgtcg ccgacgtggc
    13981 cgccgaggtc gccgcactcg agaacggctc ggtcaacgcc ctcgtcaccc agccccccac
    14041 gggaaccaag acgcgggaca agggcttcca caagatcatg gacttcacca actaccccgt
    14101 cgcggcaaac gccttcgcgg tcatgaccga ctacatcgac aagaacaagg aagccgtcgc
    14161 agccttcgtc aagtcggagg tcgagtgcct cgccctgctg cacaaggaca aggcgaaggg
    14221 cgtggccagc atcaccaagc acctcaacct gaccgacgcc agcctggccg agtactccta
    14281 cgactacttc gagaagatct gggccaaggt gccgaccgtg cagccagacc tcatcaaggc
    14341 gtccttcgaa gaggcggcga gcaaggccaa ggtcgacgca cccgccgaca tcacaaagta
    14401 cgtcgagaac agcttcatcg aggagctgga gaagtcgggc ttcatcgact cgctctacaa
    14461 gtagccatca cgacggccgg ctccgagaca gccggcccgg acacgggttg cgggaccacc
    14521 tcggcgaatc gccggcgaag ggccagagat ctggctggtg gtcccgcaac cacgaaaggg
    14581 gccagctctc gtgacggagg acttcgacct cgtcgaggcg agcattgcag gaatccacga
    14641 ggcctacctg tgccgggcac tgtcgtgccg cgagctggtc gagggatacc tcgagcgcat
    14701 tcaggcgtac gacgctgccg ggccccggct caactgcatc atcaccgtca accctgacgt
    14761 gcgcgcacag gccgccgagc tcgacgccgc gctcgctacg ggtgacctgg cggggccgct
    14821 gcacggcatt cccatcgtgg tcaaggacca gatcgacgtc gccggaatgc ccaccacgat
    14881 gggctccacg ctctttgccg actacgtccc cggcagggac tcctcggtcg tctccaggct
    14941 caaggctgcc ggcgccctca tcctggcgaa gacgacgctc ggcgagctcg gcgccgggga
    15001 cacgcacggc agtctcttcg gctcgacacg caacccctac cggctcgagc acacaccggg
    15061 tgggtcctcc ggagggtctg ccgtggcggt cgccgccaac ctcacgacgg cggctctagg
    15121 gcaggagggc ctcgcctcga tcacccggcc gtcggcctgg aactcgatcg tcgggatgcg
    15181 gccgacggcc ggcttggtga gccgggcggg cgcctacagc ggctggccca accgcgccag
    15241 ctcgatcggg ccgatggccc ggaccgtcga ggacgccgcc cggctgctcg acgtgatcgc
    15301 cggctacgac ccggacgacc ccctcacggc cacgggcgtc ggccacctgt ccgggcccac
    15361 ggtgggccag ctctgcccga aggcgctgga cggtgcgcga gtgggcgtgc tccgccagag
    15421 catcggcctg gggtcggagc ccgactcgga ggacttcacc cgcgtgagcg tggtcttcga
    15481 ccgagccatc gccgacctac gggcggccgg cgccatcgtc gtggaccccg tcgaggtgcc
    15541 ccggctgcaa gagctgctcg accagcgagc gttcgagcac gccgaggcgt cgttcgaggc
    15601 gtggatggcc cgcagcgagc acccgcccta tcgctcgcac gccgagctcg ccgcgcagcc
    15661 ggcctaccaa gacctggtcc atcggcgctc gggcggtcat cttcgccggt tcgggagcag
    15721 ctctcactac gcctacctgc gcgcgcgtga cgagctgcgc atcaacctgc tcaaggtcat
    15781 ggccgatgcc cggctcgacg tcctcgtgct caagtcggtc gagcacaccg ccacgcccat
    15841 cagcgagggc acggccccgc cgttcaccga cgggaagggg gcgatccacc tcgcgacctt
    15901 cttgatcgac gtcccttcga tcaccgtccc ggccggtgcg acacccgacg gcctgccggt
    15961 cggcctctcg ttccttggcc gacccttcac agacgctgcg gtgctcggtt atgcctacgc
    16021 ctatgagcag cacacccggc gccggcggcc acccgagggc ttcccgccct tgcgctgacc
    16081 cgcctcgccc gtggtcacgc gccgtggacc gacagcgtcc cgtactggtg catcgtcgaa
    16141 tggatccccg cctccaccgc gtcggcatgc gcccgcatgg ccgcggcgat cgcctcggcg
    16201 tcccgatcct tgatcgcggc cacgatcttc tcgtgctccc gtaggcgctc cgcggcgtga
    16261 aagccgcggt cgatgagcag gtagtgggag atgagcaggt gaaggttgag cgaggcgtag
    16321 tgggcaagca ggtagggatt gtttgcgcag gccatcgaga gccggtggaa cttcacgtcg
    16381 gcgtcgatga aaccaaccat gtcggcgacc gactcgccat cgttgccgtc cgacttcgat
    16441 gacgtcaggg tcgaggccgc ctccgccagc tcttggaact gctcgtcagt catccgctcg
    16501 gcgatcagcg gagcagcggc ggtctcgtag atgcgccgca tctcgaacag gttggtgatg
    16561 tcatcgtcgt cgagccgggc cacacgcgtg ccgctgcgtc cgcgcacctg gagcagaccc
    16621 tcgacggcca gccgctcgat ggcactcttg accggggcac ggccgagacc ggtcgccgcc
    16681 gcaaggtgcg cgacggaggt tcgttcgccc ggacgcagct cctggtgcag gagcattatc
    16741 cggattcttc gatacgccgc ctccgtaaga gtcccccagg ttgcggctgc atctggcacg
    16801 atgtgtcctt ccattgcgat ggtcccaaac cgggcaagcc tagcagtcgc cgcgggtgtc
    16861 aagcccttgc gcgacaacgt cgtcatagct cacctcggcc gagcacatcc cggctcggcg
    16921 tgcaaacccg ttgagcccgt ccaccgcctc gtgactgatc ttcgcatcgt agaacggcag
    16981 atcacgccgt accaggtcgg tgatgaagcc cgcctcggcg gccggaaaga gccggcggcc
    17041 cacggcggcg gcccgttggg ggtcttcctt caatgcctgc tgggcgccga cgatgccgcg
    17101 gaccagcgcg gcgacctgtc gggggtgctc ggcgatgacg gcatcggttg ccatcgccgc
    17161 cgcaaaagtg aaggaacgtg ctcgggcggg gccttgaccg cgccgtgggt cgagaaagac
    17221 ccggccggct ccgtcattga ccgctatctc agcagccatg gcatttgccc agaatgcatc
    17281 gatgcggccg tcgcgcaggg cggcggccgc gctgagaccg aacgacgtgt cctggccctc
    17341 ggaggccgga gggggcccga tcgtgatccg gttgtcgtcg agactgacgt gcgcctcggc
    17401 gaagagctgg cgcaacgcct gctccggacc acgggcggcg gcgattcgta ggtcgtggag
    17461 tcccgccagg tcacccggcg cgacgtcgag atcacaccgg acgacgagca gccagtagat
    17521 gtgctgggcc accgcggcga gcagcttgac accggcccag tcgggaaatg ccttcagcgg
    17581 cgcgtgagca ggccccacga gaaagtcgat ctctccgtcg cgcagcgcga tggcggcgct
    17641 ggtcacggga tagacctgct ggagctcgac atcgacgccc tcctgggcga acagaccgag
    17701 ctcggcggcc gcaaggaccg ggaaatagga agaggaaacg aggtcgggga ccgctaggcg
    17761 catcggcg
//
LOCUS       DATIBB010000042         7905 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_1727751, whole genome shotgun sequence.
ACCESSION   DATIBB010000042 DATIBB010000000
VERSION     DATIBB010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 7905)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7905)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7905
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1727751"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..620)
                     /gene="mshD"
                     /locus_tag="VLC50_01475"
     CDS             complement(<1..620)
                     /gene="mshD"
                     /locus_tag="VLC50_01475"
                     /EC_number="2.3.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012887644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035447 - mycothiol synthase activity
                     [Evidence IEA];
                     GO_process: GO:0010125 - mycothiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mycothiol synthase"
                     /protein_id="HSF26164.1"
                     /translation="MPEPDHDVRLQASGRLDGAEVSAVRRIVEAATELDGVRPLSEHV
                     MLHLRYGGDEPGRNVLLWEDDELAAYAHLDVTDTVEGPSAELVVAPAHRGRGLGRRLV
                     ETLIEASPDGRLRLWAHGETSAAVELALGMGFVQMRTLWQMRRSLYAALPPVEAPYGV
                     VVRTFRPGEDDEAWAALNARAFATHPEQGTWTLEDLHRRMGEPWFDP"
     gene            complement(835..1518)
                     /locus_tag="VLC50_01480"
     CDS             complement(835..1518)
                     /locus_tag="VLC50_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020552265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HSF26165.1"
                     /translation="MRSLLLLTHTLAPAAEVLPALGLLQHRVRVAPADGAALVDAPAA
                     DVVLVDGCHDLPHARSLCRLIRTTGIACPLLLVTTAGGLAAVSADWGVDDVLLDTAGP
                     AEVEARLRLAIGRLQAAREDQDDVEEIRAGELVVDEATYTARLRGQVLDLTYKEFELL
                     KYLAQHPGRVFSRAQLLSEVWGYDYFGGTRTVDVHVRRLRAKLGPEHEALIGTVRNVG
                     YRFVAVPVG"
     gene            1683..1940
                     /locus_tag="VLC50_01485"
     CDS             1683..1940
                     /locus_tag="VLC50_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016472297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HSF26166.1"
                     /translation="MATGTIRYWAAAKAAAGLAEEGYDAATLGEALDDAAKAHGPVLE
                     QVLARCSFLVDGVRADRTVALTDGLVVEVLPPFAGGALVPR"
     gene            1988..2512
                     /locus_tag="VLC50_01490"
     CDS             1988..2512
                     /locus_tag="VLC50_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020544704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HSF26167.1"
                     /translation="MSTASTGDEELVRALYAEHAGPLLGYVLRLTGGDRYRAEDVVQE
                     TLLRAWRHPEALAAATGSPRPWLYTVARHLVVDGVRARNARPIETSGEHLAEIPVDDD
                     ELDRALTSWEVADALATLTPDHRAVLVETYFRGRSVAETATVLGIPAGTVKSRTYYAL
                     RALRLALEERGLAP"
     gene            2509..3207
                     /locus_tag="VLC50_01495"
     CDS             2509..3207
                     /locus_tag="VLC50_01495"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HSF26168.1"
                     /translation="MTCEQARISLGVYVVGALDDAERAAVRAHLETCRGCRDELAELA
                     GVPRLLARVTLAEAVHGPAQPDDDLLPRLLTRMAAERARHRRQRLVLASAAAVVVLAL
                     VASLVGVLAGRGTSDSPASPPVAAASTTPTWSASDATTGVWASVSVTPVAWGSRVHLD
                     LGGVAGGSRCQLVAVSRAGDREVAATWVVPPGGYAKGGPGLLVDGGVSLPGGELARFE
                     VVTLDGQLLVTVPV"
     gene            complement(3191..5206)
                     /locus_tag="VLC50_01500"
     CDS             complement(3191..5206)
                     /locus_tag="VLC50_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013422049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HSF26169.1"
                     /translation="MAQLHIQLLGGFRVEVDGRAVDDVVWRRKKPAAVVKLLALAGSR
                     RLHREQLQELLWPELDGAAAGANLRKAVHLARRGLEDVGGAEFLASGADAVWLPRESV
                     QIDVHEFRRTVAQSRRTADVEDYRRAVALYAGQLLPDDRYEEWATAPREELASEYLEV
                     LEELTALLGSRGELAEAITIARLAVAEDPLREDNSMTLMRFLALAGRRADALAVFKKL
                     RARLDDELGVSPSAAAQSLYEEIRSRHVLAASLAADEWEQVGDLRMMAGDATGAATAY
                     QSALAAADADAEGRVERKIADAWLMGHRPDRAVAHLDVAEARAGRGPELSMVLRSRAN
                     AAWETGDISTAQRYAERARAVAEEVGTADDLAAANEALAVVWHMSGAWRDGLTAELDR
                     LATEDDSETVMARVFDFHHCVGQYHLYGDGLSETVEDYARRILGRAEQAGAARAQAFA
                     WCLLGESLLLQARWEEAAGCLERSCDLHAGFGSSSGALPWQRRAEAAVSVGDYAEAES
                     ALRRAAAIATVSPMAAHVWGRIYATRAFAALERGDPDRAVAAVRAASAAAARYGDCAS
                     CSALLNPIAAEAFAVVGDHDSAQAYAGSAAQVAGTFASSAWSAMSQTAAAWAAYAGGD
                     TDTACERLTAAAELYRQARQPYWADRAERGFGASGSTAGHTALRPAR"
     gene            complement(5451..6860)
                     /locus_tag="VLC50_01505"
     CDS             complement(5451..6860)
                     /locus_tag="VLC50_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003896784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HSF26170.1"
                     /translation="MIFTQHYLGCLSHASYLIGDDTTGRAVVIDPQRDIEIYLTEARA
                     KGLRIERVLETHLHADFLSGHLELAAATGALISFGEAAQLEFPSEPLAHGQRLELGRV
                     VLEIRATPGHTPESICIVIYENPEDDIPYGVLTGDTLFVGDVGRPDLLASSGAGYDAS
                     TLARLLYRSLHEQVLTLPNETRVFPAHGAGSSCGKQLSSQTSSTIGEQRRSNYALQPM
                     REDQFVETVTEGQPTRPHYFSFDAQANRQTHGLLDATVPPPSMSLDEVLTRIEAGAIA
                     LDTREPADFATGHLRGAINVGLQGRFAEWAGDVLDPDCEIVLVGEPAAALESRVRLAR
                     IGFDRVVGCLDDPSSLLIDHPDLVEASSRLTIDQLAELLELESTIQLVDVRGTAETAE
                     GTLIGARVIPLAALVESLGLLDPLAPIVVNCASGYRSVIAASVLRHAGFTDVSDLIGG
                     YGAWAGARLPIHRQPVGAR"
     gene            complement(6857..7390)
                     /locus_tag="VLC50_01510"
     CDS             complement(6857..7390)
                     /locus_tag="VLC50_01510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF26171.1"
                     /translation="MDRPADPAATSHHEGNATITMTDFRHPSDLIHNTALIADPAMFA
                     KEAGAASWLYLCGEEFAAMEGGMAYADVQSAVPESANLIADPPHPFDMDVARRGIAAL
                     DKLPRPTLVTCRTGPRSSALTYLYAGLRSGASADDVLARAEADEAPFIRVEPLKAWVR
                     QGLAQLSKGHQDEGSEQ"
     gene            7688..>7905
                     /locus_tag="VLC50_01515"
     CDS             7688..>7905
                     /locus_tag="VLC50_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011718899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HSF26172.1"
                     /translation="MTTARERVLVDADWVEAHLADPKVVLVEVDEDVSAYDKGHIRGA
                     VKLDWREDLQDPVRRDFVDKAGFEALVSA"
BASE COUNT         1132 a         2811 c         2807 g         1155 t
ORIGIN      
        1 gggtcgaacc acggctcgcc catccgccgg tgcaggtcct ccagggtcca ggtgccctgc
       61 tcggggtggg tggcgaaggc gcgcgcgttc aacgccgccc aggcctcgtc gtcctcgccg
      121 gggcggaagg tgcgcaccac cacgccgtac ggcgcctcca ccggcggcag cgcggcgtag
      181 agcgaccggc gcatctgcca cagcgtgcgc atctgcacga accccatgcc cagcgccagc
      241 tccaccgccg ccgaggtctc gccgtgcgcc cacagccgca gccgaccgtc ggggctcgcc
      301 tcgatcagcg tctcgaccag ccgccggccc agcccgcgcc cgcggtgcgc cggcgccacc
      361 accagctcgg cgctggggcc ctccaccgtg tcggtgacgt cgaggtgggc gtaggccgcg
      421 agctcgtcgt cctcccacag caggacgttg cgcccgggct cgtcgccgcc gtagcgcagg
      481 tgcagcatca cgtgctcgga cagcggccgg accccgtcaa gctcggtggc cgcctcgacg
      541 atccggcgta cggcgctcac ctccgcgccg tcgagccgtc cggaggcctg cagtcgcacg
      601 tcgtggtccg gctcgggcac cccgtcacct tatgcggcac ctcgtgtcgg ggacagccgc
      661 ccgccatgcc ctgatgagac acgattgtta cgccggaccg gcgtggtgcg ctgccgtgaa
      721 tcgctacggt ttccgcatct ccgcgaaata ccaggggact cgagggaacg agggcctcgc
      781 gacttcggtc gttcgaggcc ctctctcgtt ggtggctcgc gctggcgcgg ccgctcagcc
      841 gacggggacc gcgacgaacc ggtagccgac gttgcgcacc gtgccgatca acgcctcgtg
      901 ctcgggcccc agcttggcgc gtagccgacg cacgtgcaca tcgacggtgc gggtgccgcc
      961 gaagtagtcg tagccccaca cctcggacag cagctgggcc cggctgaaca cccggcccgg
     1021 gtgctgggcg aggtatttga gcagctcgaa ctccttgtag gtcaggtcga gcacctgccc
     1081 gcgcagccgg gccgtgtagg tcgcctcgtc gacgaccagc tcaccggcgc ggatctcctc
     1141 gacgtcgtcc tggtcctcgc gggcggcctg cagccggccg atcgccagcc gcagccgggc
     1201 ctccacctcg gccgggccgg cggtgtcgag cagcacgtcg tcgacccccc agtcggcgct
     1261 caccgcggcc agcccgcctg cggtcgtgac cagcagcaat gggcaggcga tgcccgtcgt
     1321 acggatcaat cggcacagcg accgggcatg cggcaggtcg tgacacccgt cgacgagtac
     1381 gacgtcggcg gcgggagcgt cgacgagagc ggcgccgtcg gcgggagcga cgcggacgcg
     1441 atgctggagc agccccagcg cgggaaggac ctcggcggcg ggggcgagcg tgtgcgtcag
     1501 cagcagaagg ctgcgcatct cgcctccctc acgtccgggg caaacccgcg gatcgtggca
     1561 cgtgcgcgag ccggaccgtc ggttgcgagg atatcggacg ggtacgcccc gtgacgggcg
     1621 acgcgggccg atgcggttca tggcaggctc gagggaccga gcgggcgaga cgggagcggc
     1681 agatggcgac gggcacgatc cgctactggg cggcggccaa ggcggcggcc ggcctggccg
     1741 aggagggcta cgacgcggcg acgctgggtg aggcgctcga cgacgctgcc aaggcgcacg
     1801 ggccggtcct cgagcaggtg ctggcccgct gctcgttcct ggtcgacggc gtgcgcgccg
     1861 accgcaccgt ggcgctgacc gacgggctcg tcgtcgaggt gctaccgccc ttcgccggcg
     1921 gcgctctcgt cccccggtag acggggcgac gtgaaccggt gcccaccgac gtacgtttct
     1981 cgcactgatg agcaccgcat cgaccggtga cgaggagctt gtccgggcgc tgtacgccga
     2041 gcacgccggg ccgctgctcg gctacgtgct gcgcctgacc ggcggcgacc gctaccgcgc
     2101 ggaagacgtc gtgcaggaga ccctgttgcg cgcctggcgt caccccgagg cgctggccgc
     2161 ggcgaccggc tcgccgcggc cctggctcta caccgtggcc cgccacctcg tcgtcgacgg
     2221 cgtgcgcgcc cgcaacgccc gcccgatcga gaccagcggc gagcacctgg ccgagatccc
     2281 cgtcgacgac gacgagctcg accgggcgct gacctcgtgg gaggtcgccg acgcgctggc
     2341 caccctcacg cccgaccacc gggcggtgct ggtggagacc tacttccgcg gccgttcggt
     2401 cgccgagacc gccaccgtgc tgggcatccc ggccgggacg gtcaagtcgc gcacctacta
     2461 cgcattgcgt gcgctgcgcc tcgcgctgga ggagaggggg ttggcgccgt gacctgcgag
     2521 caggcccgga tctcgctggg cgtctacgtg gtcggcgcgc tcgacgatgc cgagcgggcg
     2581 gcggtgcggg cccacctgga gacctgtcgc ggctgtcgcg acgagctggc cgagctggcc
     2641 ggcgtgccac ggctgctggc ccgggtcacc ctcgccgagg cggtgcacgg gccggcccag
     2701 cccgatgacg acctgttgcc acggctgctg acccggatgg ccgccgagcg ggcccggcac
     2761 cggcgccagc ggctggtgct ggcctcggcg gccgcggtgg tggtgctcgc cctggtcgcc
     2821 tcgctggtcg gggtgctcgc gggtcgcggt acgtcggact cgccggcgtc gccgccggtc
     2881 gcagcggcct cgaccacgcc gacctggtcg gccagcgacg ccacgaccgg ggtgtgggcc
     2941 agcgtctcgg tcacccccgt cgcctggggc agccgggtgc acctcgacct gggcggggtg
     3001 gccggtggca gccgctgcca gctggtcgcg gtcagccggg ccggcgaccg cgaggtggcg
     3061 gcgacctggg tggtgccgcc gggtggctac gccaagggcg ggccaggcct gctcgtcgac
     3121 ggcggggtct cgctgcccgg gggcgagctg gcccggtttg aggtcgtcac cctcgacggc
     3181 cagctgctcg tcaccgtgcc ggtctgagcg ccgtgtgccc ggcggttgaa cccgaggccc
     3241 cgaacccacg ttcggcgcgg tcggcccagt aaggctgccg tgcctgtcgg tagagctcgg
     3301 ccgcggcggt gagccgctcg caggcggtgt ccgtgtcgcc gccggcgtag gccgcccagg
     3361 ccgctgccgt ctgggacatc gccgaccagg ccgagctggc gaaagtcccg gcgacctgcg
     3421 ccgccgaacc ggcgtaggcc tgcgcgctgt cgtgatcgcc gaccacggca aacgcctcgg
     3481 ccgcgatcgg gttgagcaac gcgctgcatg atgcgcagtc gccgtagcgc gcagcggcgg
     3541 cggaggcggc ccgcaccgcg gccacggccc ggtccgggtc cccccgctcc agtgccgcga
     3601 acgcgcgagt cgcgtagatg cggccccaca cgtgggccgc catgggcgac acggtggcga
     3661 tggccgcagc gcggcgcagc gcggactctg cctcggcata atcgccgacg gacaccgctg
     3721 cctccgcgcg ccgctgccat ggcagggcac ccgacgacga cccgaatccg gcatgcaggt
     3781 cacagctgcg ctccaggcat ccggcggcct cctcccaccg cgcttggagc aggagcgact
     3841 ccccgagcag gcaccaggcg aaggcctgcg cgcgagccgc accagcctgt tccgcgcggc
     3901 cgaggatgcg tcgggcatag tcctcgaccg tctcgctgag accgtccccg tagaggtggt
     3961 actgcccgac gcagtgatgg aagtcgaaga ctcgggccat gaccgtttcg ctgtcgtctt
     4021 cggtcgcgag tcggtcgagc tcggcggtta gaccgtcacg ccacgcgccg ctcatgtgcc
     4081 acacgacggc cagcgcttcg ttggcggcgg cgaggtcatc ggcagtgccg acctcctcgg
     4141 caaccgcgcg ggcccgttcg gcatagcgct gcgcagtgct gatgtcgccg gtctcccagg
     4201 cggcgttggc ccggctgcgc aagaccatgg acagctcggg gccgcgcccg gccctcgcct
     4261 cggcgacgtc gaggtgagcg actgcccggt cggggcggtg acccatcaac caggcatcgg
     4321 cgatcttgcg ctcgacccgg ccttccgcgt cggcgtcggc ggcagcaagc gccgactgat
     4381 aggcggtcgc cgcaccggtg gcgtcgccgg ccatcatccg cagatccccg acctgctccc
     4441 actcgtcagc cgcgagggac gcggcgagca cgtggcggga tcggatctcc tcgtaaaggc
     4501 tctgcgctgc ggcactgggc gacaccccga gctcgtcgtc aagccttgcc cgaagcttct
     4561 tgaagacggc gagggcatcg gcccgacgtc cggccagcgc caggaaccgc atcagcgtca
     4621 tgctgttgtc ctcgcggagg ggatcctcgg ccaccgccag gcgtgcgatg gtgatggctt
     4681 cggccagctc gccgcgagac ccgagcagcg ccgtcagctc ctccagcacc tcgaggtatt
     4741 ccgatgccag ctcctcacgc ggagccgtcg cccactcctc ataccggtcg tcgggcaaca
     4801 gctggcccgc gtacagggcg accgcgcgcc ggtagtcctc cacgtccgct gttcgccgcg
     4861 actgcgcgac ggtacgacga aactcgtgca cgtcgatctg gacggactct cgcggcagcc
     4921 agaccgcgtc ggcgcccgag gcgaggaact ccgcgccgcc gacgtcctcg agcccacgcc
     4981 gggccagatg gaccgccttg cgaaggttcg caccagcggc ggccccatcc agctcgggcc
     5041 acagcagctc ctggagctgc tcgcggtgta gccgccgact acccgccagc gcgagcagct
     5101 tcaccacggc tgccggcttc ttccgccgcc agaccacgtc gtcgaccgcc ctgccgtcga
     5161 cctcgacccg gaatcctccc aacagctgga tgtgtagctg agccaaccct gaccccccga
     5221 aaccacgtcg ttccgtcgcc agcccgggcg atggccacgc atcgtcggcg gtcgctgccg
     5281 ggtcaccccc gaaggtatcg ccacgatccg acaatggatg ctcgaggagc cgctccgcgg
     5341 gctggtgtct taacacaggt ctgcagggtc gtgctgcttt cacatgttca ggcgcggaac
     5401 cttagggctc cgcgcctgaa cggttggggg gcttgatcac gtgtgagcgc ttatcgggca
     5461 cccaccggtt gccggtggat cggcaggcgg gcgcccgccc atgcgccgta tccgccgatc
     5521 agatccgaca cgtcggtgaa cccggcgtgt cggagcacgc tggccgcgat caccgaccgg
     5581 tagccgctgg cacagttgac gacgatcggc gcgaggggat ccagcagtcc caaggactcg
     5641 accagtgcgg cgagcgggat cacacgcgcg ccgatgaggg tgccttccgc cgtctcggcc
     5701 gtgccgcgca catcgaccag ctggatggtg gactccagct cgagcagctc cgcgagctga
     5761 tcgatcgtca atcgcgagct ggcctcaacc aggtcaggat gatcgatgag cagactgctc
     5821 gggtcatcga ggcagcccac gacacggtcg aacccgatcc gggccagccg cacgcgactc
     5881 tccagcgcgg cagccggctc acccaccagc acgatctcac aatcagggtc gagcacgtcg
     5941 cccgcccatt cggcgaaacg gccctgcagc ccgacgttga tcgccccgcg caggtgcccg
     6001 gtggcgaagt cggccggctc tctcgtgtcc agcgcgatgg cgccagcctc gatccgggtg
     6061 agcacctcat cgagggacat gctgggcggg ggcacggtcg cgtccagcag cccatgcgtc
     6121 tgccggttcg cttgtgcatc gaacgagaag tagtgcggtc gggtcggctg gccctcggtc
     6181 acggtttcga cgaattggtc ctccctcatc ggttgcaacg catagttgga ccgccgctgc
     6241 tcaccgatgg tggagctggt ctggctggac agctgcttgc cgcaggacga cccggcgccg
     6301 tgcgcgggga acacgcgtgt ctcgttcgga agggtcaaca cctgctcgtg cagcgaacgg
     6361 tagagcaggc gcgcgagtgt cgacgcgtcg tatccggctc ccgacgacgc gagcagatcc
     6421 ggccggccga cgtcaccgac gaacagcgtg tcgccggtca ggacgccgta cgggatgtcg
     6481 tcctcggggt tttcgtagat cacgatgcag atggattcgg gggtgtgacc gggcgtggcg
     6541 cgaatttcca gcacgacccg accgagctcg aggcgctgac catgggcgag cggttccgag
     6601 gggaactcga gttgggccgc ctcgccgaac gagatcaggg cgcccgtcgc agccgccagt
     6661 tccagatgtc cggataggaa gtcagcgtgc agatgggttt ccagcacccg ttcgatgcgc
     6721 aggcccttcg cccgggcttc tgtcaggtag atctcgatgt cgcgctgcgg atcgatgacc
     6781 acagcccggc cggtcgtgtc gtcaccgatc agatacgagg catgggagag gcaacccaga
     6841 tagtgctgag tgaagatcat tgttctgacc cttcgtcttg gtgacccttg gacagttggg
     6901 ccagcccctg ccggacccac gccttgagcg gttcaacccg gatgaacgga gcttcgtctg
     6961 cttcggcacg agcaagcacg tcgtcggcgg aagcaccgga ccgcaggccc gcgtagagat
     7021 acgtcagcgc cgaggagcgg gggccggtgc gacaggtgac aagggttggt cggggcagct
     7081 tgtccagggc cgcgatcccg cgtcgcgcga catccatgtc gaacggatgg ggcgggtccg
     7141 cgatgagatt ggcggactcc ggtacggcgg actggacgtc ggcgtaagcc atcccgccct
     7201 ccatggcagc gaactcctcg ccgcacagat acagccagga cgcggcgccg gcctctttcg
     7261 cgaacatggc cggatcggca atcagcgcgg tgttgtggat caggtcgctc gggtgtcgga
     7321 agtcggtcat ggtgatggtc gcattcccct cgtggtggct tgtggcggcc ggatcggccg
     7381 gtcgatccaa gctcgcgtcg tggtggacaa gccgaagcta ggagcggtgc gttccctggc
     7441 cgttccctcg caggcgcccc cgcagccgac tcggtgcacg atgcaggtct gcaccacggc
     7501 ctgagaacgt gaacaccctc ggtggatcgg gcgaagcatc tgaccgctcg gcggcgggaa
     7561 cgctcaggga acggacccct cctagcgttc gggttgttca cgggctgcga gatgtacctg
     7621 ctgatcaagc gcgtcagcgg tcgtcgtacg tccattcaac cgatctgagt tctggaggag
     7681 aactgtcatg accactgcac gtgagcgtgt cctcgtcgac gccgactggg tggaggccca
     7741 cctggccgac cccaaggtcg tgctcgtcga ggtcgacgag gacgtctcgg cctacgacaa
     7801 gggccacatc cgcggcgccg tcaagctcga ctggcgcgag gacctgcagg acccggtgcg
     7861 ccgcgacttc gtcgacaagg ccggcttcga ggcgctggtc tcggc
//
LOCUS       DATIBB010000043         5261 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_1859324, whole genome shotgun sequence.
ACCESSION   DATIBB010000043 DATIBB010000000
VERSION     DATIBB010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 5261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5261)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5261
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1859324"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..861)
                     /locus_tag="VLC50_01520"
     CDS             complement(<1..861)
                     /locus_tag="VLC50_01520"
                     /inference="COORDINATES: protein motif:HMM:NF014795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HSF26173.1"
                     /translation="MKVLSAAAAALRDHGIDTMFGVMGDANMYLVDRFVRDHGGRFFS
                     TAHEAGGVMMAAGYAAVTGRPAAATATHGAVTNMVSALFDATRGGYPLVVILGDTGAR
                     DGYHLQNLPHKEIVAPTGARFIDVRSASTVVEDIARAVRWAQTESRPVVVNLPIDIQN
                     EDVDYTKVSWGALPSRVWGPEPSAVEDAVGLISAARRPVVIGGRGAADPAAREAVARL
                     AERIGAPVASTLRGKDLFRGDPFNLGICGGLATPLGTEIIANADTLIAFGASLSALTA
                     DYGDLIAGKRI"
     gene            complement(991..1770)
                     /locus_tag="VLC50_01525"
     CDS             complement(991..1770)
                     /locus_tag="VLC50_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009721048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HSF26174.1"
                     /translation="MPPTSEPGRSVSSRLLDVLFAFQPGRARLNLAELTRHTGLPRAT
                     VRRLAMELVQAGALERGEDGRFSVGLRLWQLGTLAPFTESLRTTARPYMEDLYGALRQ
                     HVQLAILEGREAVIIERLSHRKAVEVVSQVGGRLPLHCSGVGKVLLSHSGAAFIDGVL
                     QTKLHRYTPKTQTDPARLRAELAACRRTGTAVVNEELTLGADSVSARIMNGQGDVLAA
                     LSVVVRTGSVSAQAVLPTLITSALAISRTFGWHPGVRPRVH"
     gene            1925..3142
                     /locus_tag="VLC50_01530"
     CDS             1925..3142
                     /locus_tag="VLC50_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HSF26175.1"
                     /translation="MIGNAEQPRIAIVGGGIGGLAAAAFLHRVGLASTVYEQAHELGE
                     VGAGLVVAPNAARLLRRLGVLDSLVKTAVKLEVGWEFRRWRDGEVLSAEDLSAACEDL
                     YGEHTYTAHRADLLNAVRSAVPDAFIRLGKRCVGLDSEGPHPVLRFADDTSVSADVVI
                     GADGIHSVTRGALVESGDPTSSGLCAFRSLVPVADAPAFARRQAQTLWIGPGHHLVHY
                     PISSGAFVNLVAFAPAGDYSVESWSATATTAEFLAEFEGWDPRLGELIRAGGTPGRWA
                     LLDREPLQRWSFGPVTLLGDAAHPMFPFFAQGAAQSIEDAVVLAQCLAEDTSEPARAL
                     ARYEAVRRERATRIQRASHARSDINHLPDGPAQAERDKSFTAADPLVANGWIYGHDPE
                     AALREPAAAPAGQ"
     gene            3159..4214
                     /locus_tag="VLC50_01535"
     CDS             3159..4214
                     /locus_tag="VLC50_01535"
                     /EC_number="1.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019074005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018506 - maleylacetate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylacetate reductase"
                     /protein_id="HSF26176.1"
                     /translation="MDFAHEWPAQRVVFASGAARRALATEVAALGASRVMVLAAEAER
                     PLADEVTAGLPVALRHHEVVMHVPVEVAERARKAAATHQVDALVCVGGGSTTGLAKAI
                     ALTSSLPIVAVPTTYAGSEATSVWGMTEASRKTTGADPRVLPRAVVYDAALTLSLPVA
                     LSVASGLNALAHCVDSLWAPRADPLDRALAGEGIRALAAGLPAVVANPDDLAGREQAL
                     LGAYLAATAFSSAGSGLHHKICHVLGGMFNLPHAQTHAVVLGHVLALNAPNAPQAAAR
                     VANALGAPDALSGLAALRDRLDAPRALRDLGLAEDDLQAAVDPILAAAPADNPTPLTR
                     DTITRLLHAAWEGAEPR"
     gene            4211..5134
                     /locus_tag="VLC50_01540"
     CDS             4211..5134
                     /locus_tag="VLC50_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012691473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0018576 - catechol 1,2-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0009712 - catechol-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dioxygenase"
                     /protein_id="HSF26177.1"
                     /translation="MSTPVDALAAPEPEPLPAEQEQRELDLIRWVLSSFQSCDEPRLK
                     QLMQALVRHLHAFLREVRLTEDEWWSAIHFLTDAGHITDDQRQEFILLSDVLGASMQT
                     IAMNNRNYADATEATVFGPFFVDGAPQIELGGDITGASRGTPCWVEGTVTDTDGRPLP
                     HARIDVWESDEDGHYDVQYADGRVGGRAHLFTDDEGGYRFWGIVPTPYPIPDDGPVGK
                     MLRATGRSPMRAAHLHFMVAAQGCRRLVTHVFPADDPLLTSDTVFGVRQSLVKDFQPQ
                     PTGTPTPDGRDLEGRDWYRLRFDIVLAPQGV"
BASE COUNT          730 a         1921 c         1882 g          728 t
ORIGIN      
        1 gatccgcttc ccggcgatca ggtcgccgta gtcggcggtg agcgcgctca gcgacgcacc
       61 gaacgcgatg agggtgtcgg cgttggcgat gatctcggtg ccgagcgggg tggcgagccc
      121 gccgcagatg ccgaggttga acgggtcgcc gcggaacagg tccttgccgc gcaaggtcga
      181 ggcgacgggt gcgccgatcc gctcggccag gcgggccacc gcctcgcggg ccgccggatc
      241 ggcggcacct cggccgccga tgaccaccgg ccggcgggca gcggagatca gtccgacagc
      301 gtcctcgacg gccgacggct ccggccccca gacccgtgac gggagcgcgc cccagctgac
      361 cttcgtgtag tcgacgtcct cgttctggat gtcgatcggc aggttgacca cgaccgggcg
      421 agactcggtc tgcgcccagc gcaccgcgcg cgcaatgtct tccacgacgg tgctcgccga
      481 acgaacgtcg atgaagcggg caccggtggg cgccacgatc tccttgtgcg gcaggttttg
      541 caggtggtag ccgtcccgcg cgccggtgtc gccgaggatc accacgaggg ggtagccacc
      601 gcgggtggcg tcgaacaggg ccgacaccat gttggtcacc gcgccgtgcg tggcggtcgc
      661 cgcggccgga cgtccggtca ccgcggcgta gccggcggcc atcatcacgc cgccggcctc
      721 atgcgccgtg gagaagaagc ggcccccgtg gtcgcgcacg aaccggtcga ccagatacat
      781 gttggcgtcg cccatgacgc cgaacatcgt gtcgatgccg tggtcgcgga gcgctgctgc
      841 ggccgcggaa agcaccttca ttgctgcacc caccatcctg tcggagacga ccactccggc
      901 cgagtctgcg gcgtgactcg gcgcaggcgg aaccaggggg tcattgaacg gttggcatca
      961 cgccggcgtc gaggccacgg gaatctaacg tcagtgaacc ctcggtcgta cgccggggtg
     1021 ccagccaaac gtgcgcgaga tcgcgagcgc gctggtgatc agcgtgggca gcacggcctg
     1081 agcgctcacc gagccggtgc gcaccacgac ggacagggcg gccagcacgt cgccttgtcc
     1141 attcatgatc cgcgcggata cggagtcggc gcccagcgtg agctcctcgt tgacgaccgc
     1201 tgtgcccgtc cggcggcagg cggcgagctc ggcccgcagc cgcgccggat cggtctgggt
     1261 cttgggcgtg tagcggtgca gcttggtctg gagcacgccg tcgatgaacg cggccccgct
     1321 gtggctgagc agcaccttgc ccacgcccga gcagtgcaac ggcagccggc cgccgacctg
     1381 ggagaccacc tcgaccgcct tgcgatggga cagccgctcg atgatcaccg cctcgcgccc
     1441 ctcgaggatc gccagctgca cgtgctggcg cagcgcgccg tacagatctt ccatgtaggg
     1501 ccgggcggtg gtgcgcaacg actcggtgaa cggcgcgagg gtgcccagct gccacagccg
     1561 cagcccgacc gagaagcgcc cgtcctcgcc ccgttccagg gcaccggcct gcaccagctc
     1621 catcgccagc cggcggaccg tggcccgcgg cagcccggtg tggcgggtca gctcggcgag
     1681 attgagacgt gcccgcccgg gctggaaggc gaagagcacg tcgagcaggc gtgagctgac
     1741 gctcctgccg ggttcactgg tgggtggcac gccgcctccg cccgggtcgt cgagtgcacc
     1801 gatcgtggcg tgcgcatcct agtccggagc gaccgttgaa tggtcgcccc gccgtaccga
     1861 gccactgact ggcggcagca tcctcagcgg acacaccaac cccgcactga agttgggcag
     1921 gagcatgatc gggaatgccg agcagccgag aatcgcgatc gtcggcggcg ggatcggcgg
     1981 actagccgcc gccgccttcc tgcaccgggt cgggctggcg agcacggtct atgagcaggc
     2041 acacgagctc ggtgaggtcg gcgccggcct ggtcgtcgcc cccaacgccg cccggctgct
     2101 gcgccggctg ggggtgctcg actccctcgt gaagacggcc gtgaagctgg aggtgggttg
     2161 ggagttccgc cgttggcgcg acggcgaggt gctctccgcc gaggacctgt ccgccgcgtg
     2221 cgaagacctg tacggcgagc acacctacac cgcgcaccgc gcggatctgc tcaacgcggt
     2281 ccgcagcgcc gtgccggacg ccttcatccg gctgggcaag cgctgtgtcg ggctggacag
     2341 cgagggcccg cacccggtcc tgcgctttgc cgacgacacc agcgtgagcg ccgatgtcgt
     2401 gatcggtgcc gacgggatcc actcggtgac ccgcggcgcc ctcgtcgagt cgggcgaccc
     2461 cacctcgtcg gggctgtgcg ccttccgcag cctcgtcccg gtggccgacg cgccggcctt
     2521 cgcccggcgt caggcgcaga ccctgtggat cggccccggc caccacctcg tccactaccc
     2581 catctcgtcc ggggcgttcg tcaacctggt cgccttcgcg ccggccggtg actactccgt
     2641 cgagtcgtgg agcgcaaccg cgacgaccgc ggaattcctc gccgagttcg aggggtggga
     2701 cccgcggctg ggcgagctca tccgggccgg cggcacgccc ggacggtggg ccctgctgga
     2761 ccgggaaccg ctgcagcggt ggagctttgg cccggtcacg ctgctgggcg acgcggccca
     2821 tccgatgttc ccgttcttcg cccagggcgc cgctcagtcg atcgaagacg ccgtcgtgct
     2881 cgcgcagtgc ctcgccgaag acacgtcgga gccggcgcgc gcgttggcgc gctacgaggc
     2941 ggtacggcgg gagcgggcca cccgcatcca gcgggccagc cacgcccggt cagacatcaa
     3001 ccacctgccg gacggccccg cccaggccga acgcgacaag tccttcacgg ccgccgaccc
     3061 gctggtggcc aacggctgga tctacgggca cgaccccgag gcggccctgc gcgaacccgc
     3121 ggctgcgccg gccggtcagt gagcgaatga tgtccggcat ggatttcgca cacgaatggc
     3181 ccgcgcagcg ggtggtcttc gcgtcgggtg cggcccggcg cgccctcgcg accgaggtcg
     3241 ccgccctggg cgcgtcacgg gtgatggtgc tcgccgcgga ggccgagagg ccgctcgctg
     3301 acgaggtcac ggcggggctg ccggtcgcgc tgcgccacca cgaggtcgtc atgcacgtgc
     3361 cggtggaggt ggccgagcgg gcccggaagg cggcggcgac ccaccaggtg gacgcgctgg
     3421 tctgcgtcgg cggcggctcg accaccgggc tggcgaaggc gatcgccctc acctcgtcgt
     3481 tgcccatcgt cgccgtaccc acgacgtacg ccggatctga ggcgaccagc gtctggggga
     3541 tgaccgaggc cagccggaag acgaccggtg ccgacccgcg ggtgctgccg cgcgccgtgg
     3601 tctacgacgc tgccctgacc ctgtcgctgc cggtggcgct gagcgtcgcc tccggcctca
     3661 acgcgctggc gcactgcgtc gactcgctgt gggcgccgcg cgccgacccg ctcgaccggg
     3721 ccctcgccgg tgaggggatc cgcgccctgg cggcggggct gcccgcggtg gtcgccaacc
     3781 cagacgacct cgccgggcgg gagcaggccc tgctgggcgc ctatctcgcg gccaccgcgt
     3841 tctcctcggc cggctccggg ctgcaccaca agatctgcca cgtgctcggc ggcatgttca
     3901 acctgccgca cgcgcagacc catgcggtgg tgctgggcca cgtcctcgcg ctcaacgcgc
     3961 ccaacgcgcc acaggcggcc gcccgggtgg cgaacgcgct cggcgcaccg gacgcgctgt
     4021 ccggccttgc ggcgctgcgc gaccggctgg acgccccccg cgcgctgcgc gatctcggcc
     4081 tcgccgaaga tgaccttcag gccgcggtcg acccgatcct cgccgccgcg cccgcggaca
     4141 acccgacgcc cctgacccgg gacaccatca cacggctgct gcacgccgcg tgggaaggag
     4201 cagaaccgcg atgagcacac ccgttgacgc cctcgctgcg ccggagccag agccgctgcc
     4261 ggccgagcag gagcagcgcg agctcgacct gatccgctgg gtgctgtcct ccttccagtc
     4321 gtgcgacgag ccgcggctca agcagctgat gcaggcgctg gtgcgtcacc tgcacgcctt
     4381 cctccgggag gtccggctca ccgaggacga gtggtggtcc gcgatccact tcctcaccga
     4441 tgcggggcac atcaccgacg accagcgcca ggagttcatc ctgctctcag acgtgctggg
     4501 cgcctcgatg cagacgatcg ccatgaacaa ccgcaactac gccgacgcca ccgaggccac
     4561 ggtcttcggg ccgttcttcg tcgacggggc gccccagatc gagctgggcg gtgacatcac
     4621 cggcgcctcg cgggggacgc cgtgctgggt cgagggcacg gtgaccgaca ccgacgggcg
     4681 cccgctgccc cacgcgcgca tcgacgtctg ggagtccgac gaggacggcc actacgacgt
     4741 ccagtacgcc gacggtcggg tgggcggccg tgcgcacctg ttcaccgacg acgagggtgg
     4801 ctaccggttc tggggcatcg tgccgacgcc ctacccgatc cccgacgacg gcccggtggg
     4861 caagatgctg cgcgccaccg ggcgctcacc gatgcgcgcg gctcacctgc acttcatggt
     4921 cgccgcgcag ggttgccgtc gcctggtgac ccacgtcttc ccggccgacg acccgctgct
     4981 gaccagcgac accgtcttcg gcgtgcgcca gtcgctggtc aaggactttc agccgcagcc
     5041 gacggggacg cccacgccgg acgggcgcga cctcgagggg cgtgactggt accggctgcg
     5101 cttcgacatc gtccttgccc cgcaaggcgt ctgaccgccg gagcgttcag gcggtcggcg
     5161 tggggtcgcc gtagaaccag tcgatgcgcc ggtagtcgtg cgggtcgcgg tcgcgcagca
     5221 gctcgttgcg cagcccccga gccagcccgt cgcagtgcca c
//
LOCUS       DATIBB010000044         2918 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_1201850, whole genome shotgun sequence.
ACCESSION   DATIBB010000044 DATIBB010000000
VERSION     DATIBB010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2918)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2918)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2918
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1201850"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..756)
                     /locus_tag="VLC50_01545"
     CDS             complement(<1..756)
                     /locus_tag="VLC50_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSF26178.1"
                     /translation="MAVARVLVGREPERQLLAALVEGARGGSAGTLVVRGEPGVGKSA
                     LLDELVAGAGEATVLRTQGLEVEAPLAFAALHRLLRPVTRLRGGLPEPQARALRVAFG
                     EEEGAAVEPFLVGVSTLSILTAAAEENAVLCVVDDAHWLDAATAGALLFCAHRLGADR
                     VGLVFAARDGAWGTFHPQGLRELVVTGLDPEASRDLLQARLGAATGAEVVERIGAETR
                     GNPLALLELPAELTPDQLSGVDPLPAQLHLTARV"
     gene            complement(823..1479)
                     /locus_tag="VLC50_01550"
     CDS             complement(823..1479)
                     /locus_tag="VLC50_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012725448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HSF26179.1"
                     /translation="MSIRVVVADDQDLVRTGLVMILGAQPDLEVVGEAADGLAALDLA
                     TRLRPDVLLVDIRMPGLDGVEVTRRLAGPDVTDPMAVVVITTFDLDEYVLGALRAGAR
                     GFLLKDAGPELLVQAIHAAANGDALIAPNATRRLLATFANQARVVPVQPIDPLTEREE
                     EVLALVARGRTNAEIATELFVGLSTVKTHVASLMTKLGARNRVEIAMWAYDTRRVRAD
                     "
     gene            complement(1476..2615)
                     /locus_tag="VLC50_01555"
     CDS             complement(1476..2615)
                     /locus_tag="VLC50_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HSF26180.1"
                     /translation="MVTVRRSIWHEPRPADAPPIGRLDWLLVGVFAAAALVEGIVRPG
                     LAWRPLVTVLALALVPALLWRRGRPLMAALVGWGVAGLLSVLQLTAHAGNLGLYSMMA
                     VLILLYSLVRWGSGREMVLGTAFVTVVVALGMYASSAGWADVFGGSVLLLLFVALAAV
                     FRYRADLWHRQQREIRNQERVALARELHDTVAHHVSAIAVQAQAGGVVAGIQPEKAAE
                     VLAAIESEASRTLAEMRSMVRVLREEEAVAYSPQLGVADLPALARADATPTVEVSLDG
                     SLTRLARPVDAALYRLAQESLTNAVRHARSATRVGIDVRREGDAVRLRVSDDGQTEPG
                     PAPEPGFGLLGMAERAQLLGGSLSAGPGPEGGWVVEAVLPVEALA"
BASE COUNT          459 a         1092 c         1020 g          347 t
ORIGIN      
        1 gacgcgcgcg gtcaggtgca gttgcgcggg caggggatcc acgccactga gctggtcggg
       61 ggtcagctcg gcggggagct ccagcagcgc cagggggttg ccgcgggtct cggcaccgat
      121 ccgctccacg acctcggccc cggtcgcggc gcccagacgt gcctggagca agtcgcggga
      181 ggcctcaggg tcgagaccgg tcacgaccag ctcgcggagg ccctgcgggt ggaacgtccc
      241 ccaggcgccg tcccgggccg cgaagaccag gcccacgcgg tccgcaccga gccggtgggc
      301 acagaagagc agggccccgg ccgtggcggc gtccagccag tgcgcgtcgt cgacgacaca
      361 cagtacggcg ttctcctccg ccgcggcggt gaggatcgac agcgtcgaca cccccaccag
      421 gaacggctcg accgcggcgc cctcctcctc gccgaacgcg acccgcaggg cgcgcgcctg
      481 gggttcgggc agtccaccgc ggagcctggt gacgggacgc agcaaccggt gcagcgccgc
      541 gaacgcgagt ggcgcctcga cctccagacc ttgagtgcgc agcacggtgg cctcgcccgc
      601 ccccgccacg agctcgtcga gcagcgccga cttgccgacg ccgggctcgc cgcggacgac
      661 cagcgtgccg gcggacccgc cccgcgcccc ctcgacgagt gcggccagga gctgccgctc
      721 cggctcgcga ccgaccagca cgcgagcgac cgccacgcga cgcaacgtac tccgcgccgc
      781 tgggctaccg accccaacgg cccacgcggc gacccgtacc cgctagtccg ctctcacgcg
      841 tcgggtgtcg tatgcccaca tcgcgatctc gacgcggttg cgggcgccga gcttggtcat
      901 caacgatgcg acgtgggtct tgaccgtgct caggccgacg aagagctcgg tggcgatctc
      961 ggcgttggtc cggccccgtg ccaccagcgc gagcacctcc tcctcgcgct cggtgagcgg
     1021 gtcgatgggc tggaccggta ccacccgcgc ctggttggcg aaggtcgcca gcagccggcg
     1081 ggtggcgttg ggggcgatca gcgcgtcccc gttggccgcg gcgtggatgg cctgcacgag
     1141 gagctccggc ccggcgtctt tgagcaggaa gccgcgggcg ccggcgcgta gggcgccgag
     1201 gacgtactca tcgaggtcga aggtggtgat cacgacgacc gccatcgggt ccgtcacgtc
     1261 tggcccggcc aggcgccgcg tcacctcgac gccgtcgagc ccgggcatcc ggatgtcgac
     1321 gaggaggaca tcgggacgca gccgggtcgc caggtcgagc gctgcgagcc cgtctgctgc
     1381 ctcccccacg acctcgaggt cgggttgcgc gccgaggatc atcaccagcc cggtccggac
     1441 caggtcctgg tcgtcggcta cgacaacacg gatgctcatg ccagcgcctc caccggcagc
     1501 acggcctcga ccacccagcc accctcgggc cccggccccg cggagagcga tccgccgagg
     1561 agctgggcgc gttcggccat gcccagcagg ccgaacccag gctccggggc tggccccggc
     1621 tcggtctgtc catcgtcgct gacacgcagc ctgacggcgt cgccctcccg gcgtacgtcg
     1681 atcccgacgc gggtcgcgct ccgggcgtgc cgtacggcgt tggtcagcga ctcctgcgcg
     1741 agccggtaga gcgcggcgtc cacgggtcgt gccagccggg ttaacgaacc gtccagcgag
     1801 acctcgacgg tgggcgtcgc gtcggcgcgc gccagagcag gcaggtccgc gacacccagc
     1861 tgcggtgagt aggcgacggc ttcctcctca cgcagcaccc gcaccatgga tctcatctcc
     1921 gccagggttc gcgacgcttc agactcgatc gcggccagga cctcggcagc cttctcaggc
     1981 tggatgccgg cgaccacgcc accggcctgc gcctgcaccg cgatggccga gacgtggtgg
     2041 gccacggtgt cgtgcagctc gcgcgccagc gccacccgct cctgattgcg gatctcgcgc
     2101 tgttggcgat gccagaggtc cgcgcggtag cggaacaccg ccgcgagggc gacgaacagc
     2161 agcaagagga cgctcccgcc gaaaacgtcg gcccagcccg cggaggaggc gtacatcccc
     2221 agcgcgacga cgaccgtcac gaacgccgtc cccaagacca tctcccggcc cgagccccac
     2281 cgcaccaggg agtagagcag gatcaggacg gccatcatgg agtagaggcc gaggttcccg
     2341 gcgtgcgcag tcagctggag gaccgagagc agcccggcga cgccccaccc gaccagggcg
     2401 gccatcaggg ggcgaccccg ccgccagagc agggcaggca ccagcgccag ggcgagcacc
     2461 gtcacaaggg gtcgccaggc caagcccggc cgaacgatgc cctcgaccag ggcggcggcc
     2521 gcgaacacgc ccaccagcag ccagtcgagc cgaccgatgg gtggggcgtc agcaggccgc
     2581 ggctcatgcc agatggagcg ccggacagtg accacggctc cagcctaggg atcggcgtgc
     2641 ctcggcgtac ccgccgaaag tacgagagag agaccatgcc atcggccagg cgaactccgg
     2701 cctccgggcc ggtgtgcccg ccgccgggct cagcgatcgt ggagctaccg atccgcacca
     2761 cagcaacgga gcccgcgatg agcacacgtc aaccgaccac cgtcacactc gaggaccagc
     2821 cggtcccagt caaggccaag ctcgctgcag cgtggacgag cttcatgttc ctgtacgcct
     2881 acgtggacat tctcggcttc tacacgccag gcatggtc
//
LOCUS       DATIBB010000045        10783 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_282688, whole genome shotgun sequence.
ACCESSION   DATIBB010000045 DATIBB010000000
VERSION     DATIBB010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 10783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10783
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_282688"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1343
                     /locus_tag="VLC50_01560"
     CDS             <1..1343
                     /locus_tag="VLC50_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016811048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GuaB1 family IMP dehydrogenase-related protein"
                     /protein_id="HSF26181.1"
                     /translation="DLATSDGTGTTIPLVVSNMTAVAGRRMAETIARRGGLAVIPQDI
                     PTDVVADVVQWVKSRHLVHDTALTLKPTDTVGDALNLLPKRAHGAVIVVDVDHCPVGV
                     VTEADCLGVDRFTQLRQVMSVELLTLPVEVDPQHAFERLQGGRHRLAPVVDEAGRLVG
                     LLTRTGALRATLYSPALDSAGRLRVAAAVGINGDVTAKASALLEAGVDLLVIDTAHGH
                     QEKMLAVLEAVRALAPAVPLVAGNVVSAEGVRDLVEAGADIVKVGVGPGAMCTTRMMT
                     GVGRPQFSAVLECAAEAARLGRHVWADGGVRHPRDVALALAAGASNVMIGSWFAGTHE
                     SPGDAHRDPDGRLYKESFGMASARAVALRTQEDSVFERARKALFEEGISSARMYVDPE
                     RPGVEDLLDAIVAGLRSACTYAGADTLAQLHERAVVGVQSAAGYAEGRPLHVSW"
     gene            1351..1776
                     /gene="arfB"
                     /locus_tag="VLC50_01565"
     CDS             1351..1776
                     /gene="arfB"
                     /locus_tag="VLC50_01565"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013284523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HSF26182.1"
                     /translation="MPEPLRVTRAVAIPAGELRWRFSRSSGPGGQSVNTTDSRAELSF
                     DVAGSPTLSPALRARALQRLAGRLADGVLTVVAAEHRSQLRNRDAALARMAALLAQAI
                     APPPRQRRATRPTRGSVERRLASKHRRSELKRSRRPDPD"
     gene            complement(1796..2644)
                     /locus_tag="VLC50_01570"
     CDS             complement(1796..2644)
                     /locus_tag="VLC50_01570"
                     /inference="COORDINATES: protein motif:HMM:NF018447.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase family protein"
                     /protein_id="HSF26183.1"
                     /translation="MRYQPKHRTPSPSHGRLVVAAATAAVSAVPVVASVAPAQAATDT
                     TWDRLAMCESSGRWQVNTGNGYYGGLQFWPATWRSFGGTAYAARADLASREQQIAVAE
                     RVLAVQGWGAWPACSAKLGLGADDARVPRASRAADRAPVLARTAPPAATSTVRGTYTV
                     RSGDTLSGIAAKLRAPGGWKALWKANQALIGANPNLLRVGTSLVVPGRAASTPAPAKA
                     SPPARTPAPSTTQGTYTVRTGDTLSRIAARAHVSGGWRALWQANRDTIGTNPNFLRVG
                     THLRLP"
     gene            2805..3923
                     /locus_tag="VLC50_01575"
     CDS             2805..3923
                     /locus_tag="VLC50_01575"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HSF26184.1"
                     /translation="MPDRAGAWKTAGLGVGLPLLLALLVLSLDLVEGPEPDFFGVLVA
                     PAFLAAALVGPAWTALTAAATAGIAFGFGFLQKNDTLTASAAFTSAQWTRLLFILVSG
                     ILAVVVATVRVQREARLARFMQISEVAQRAILPELPRRVGGLPCAGRYQSATSEAHVG
                     GDLYEVLDTAYGVRALIGDARGKGIDAVRVAGYVLGCFRAVAWSEPDLARLARSLDSA
                     VRRIGGPEDFVTAALVQLDGKDDARLVVCGHPPPLSIPPPPIAGGQSPAQPVTCDLPV
                     TPLLASEPDPPLGLLHAPPRVHRCAIAPGDRILLITDGLAEARWRGRFLDVEGTVATA
                     FRTTSLDTALDAILTTARRHVHGLVADDLALVAIQTAA"
     gene            complement(3957..4895)
                     /locus_tag="VLC50_01580"
     CDS             complement(3957..4895)
                     /locus_tag="VLC50_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018785046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP desaturase"
                     /protein_id="HSF26185.1"
                     /translation="MAELTQTQLLHALEPVVEKELNRHLTTSKEWFPHAYIPWSEGRD
                     YDGPLDGEAWDPMQAKVSEVAQTALVVNLLTEDNLPSYHREIYRVFGPDGAWGTWVGR
                     WTAEEGRHGIALRDYLLTTRAVDPVELERERMAHMTEGYNSANELDMLHTVAYVSFQE
                     LATRISHRNTGKFTEDPVADALLAKIAADENLHMLFYRNLLTGAFEVAPDQTMKAVWD
                     VVNTFAMPGAGLANFTRRSIQIAQAGIYDLRIHRDEVLAPILRKWKVWDVEGLSGEAE
                     AARERLAAFLEELDIKASRFEERREKQRARLAERVS"
     gene            complement(5010..7625)
                     /locus_tag="VLC50_01585"
     CDS             complement(5010..7625)
                     /locus_tag="VLC50_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HSF26186.1"
                     /translation="MASVPARPDAGVPGRGLTQSEAAVRLRRDGPNVLPRARPRSTAR
                     ALAAQLTHFFALMLWVAAGLAVLAGLPQLGVAIVIVVLLNGVFAFAQEWRADQAGRRL
                     QEMLPARVVVRRDARRAVVAAAELVVGDLVLLSAGDRISADLRLMVSDGLAVDESMLT
                     GESVPVPRDVDHRVFAGTFVVEGEAEAVVEATGSRTRLAEIAALTREGHRPVSPLARR
                     LHRVVWVVAVLAVATGAGFFGLALALGSPAQDGFLFAVGVTVALVPEGLLPTVTLSLA
                     RAAQRMAAQHALVRHLEAVETLGATTFVCTDKTGTLTRNEMSVVEAWTRHGSVRVEGG
                     GYTPVGRLAGSPTAVEAIRAAALVAARCSTGRVVRRGEQWQPQGDPMEVALHVLALRA
                     GADVEADERARPARRRFPFDPRRRRMSLVTTDAVLLKGAPEAVLPRCLDVPPSAAAAV
                     AGLGHRGLRVLAVAARRLADEALPADVETAECDLELLALIGIEDPPREGVAEALADLR
                     QAAIRVAMVTGDAPETARAIARETGLLGPDELVVTGADLPEDDQMLGELLDRDGVVVA
                     RVAPQDKQRIARALQGRGHVVAMTGDGVNDGPALRAADIGVAMGGSGTDVAREAADLV
                     LLDDHLATIVVAVAAGRATFTNIRRFLTYHLTDNVAELTPFVVWALSGGQVPLALGVL
                     QVLALDIGTDLLPALALGGEPPSPRVLQGPARSGALIDARLLRRVFGVLGPAEAVVEM
                     AAFVAVLLAGGWVWAATPSESLLAGASGAAFAAVVLGQLANAFACRSETSWVGQKGLR
                     GNPLLLVAVGIELVLLVGFLTVDPVASLLGGAWPPALGWALAATAIPVLLAADTLAKA
                     VRSSAARRRRTASGA"
     gene            7869..9266
                     /locus_tag="VLC50_01590"
     CDS             7869..9266
                     /locus_tag="VLC50_01590"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012950374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HSF26187.1"
                     /translation="MSIDTSMSTDRRTPAAAPSFAALGLPQRLVTALERNDIRAPFAI
                     QTLALPDALAGRDVLGRARTGSGKTLAFGLPLLARLSSSHTPGRAPRALVLVPTRELA
                     VQVRDVLEPLGHAVGLKVVAIFGGASMGRQRQALQRGLDVIVATPGRLQDFFERGDAR
                     LDEIEVSVLDEADFMADLGFLPVVSRLLDATPAHGQRLLFSATLDGAVTTIVRRYLTN
                     PVTHSVEPAAASTGSIDHRAYIVSATDKVAVLAAIASRPARTLMFVRTKHGADRLATQ
                     LGRLGVEATAIHGNLNQNQRQRALAAFTYGECRVLVATDVAARGIHVDDVDLVVHVDP
                     PSDHKDYVHRSGRTARAGASGTVVSILLPEQVRPSERLRQRAGVKVKAIRVDPGHPAV
                     RELATSGEPVVVVPRPAPEPVTHRPAGRSRRPARAGSGTGGRRGSASAAARPASAGRP
                     RRRRTDNPRSPRTAA"
     gene            9352..10368
                     /locus_tag="VLC50_01595"
     CDS             9352..10368
                     /locus_tag="VLC50_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011757200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HSF26188.1"
                     /translation="MTATATAGTSSHLDASARKELYRQMVRIRTFEEAILRDYHADKK
                     PAFDIGAGLIPGEMHLAAGQEPVAAGLCAHLRTDDAVTATHRPHHLAIAHGMDLAKLA
                     GEIYGRETGLGHGRGGHMHLFDPSTHFSCSGIIAEGLPPALGQAFAFKRAGTDRVAAA
                     VTGEGAANQGGFHESLNLAARWALPVVFVVEDNDWAISVHRDTATAVPTNAERAAAYG
                     MPGERVEDNDVEAVYDAAERAVTRARHGDGPSLIEVHTLRLWGHFEGDAQGYRPELAE
                     VPELDPIPRYEQALRAAGVLDDDEVSRIHAEATRQVGAAVDAAKAAPLPDPATATSYV
                     FADA"
     gene            10379..>10783
                     /locus_tag="VLC50_01600"
     CDS             10379..>10783
                     /locus_tag="VLC50_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011757201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HSF26189.1"
                     /translation="MTVTSSATIPGQTSGRRLTTAKAMVEAIAAEMERDPSVVYLGED
                     VGAYGGIFSSTTGLLDRFGPDRVIDTPISETAFMGLATGAAVEGMRPIVELMFADFFG
                     VCFDQIYNHMAKIHFESGGNVKVPLVLTTAVGG"
BASE COUNT         1424 a         4046 c         3890 g         1423 t
ORIGIN      
        1 tcgacctcgc gaccagcgac gggaccggca ccacgatccc gctcgtggtg tcgaacatga
       61 ccgcggtggc cggccggcgg atggccgaga ccatcgcccg ccgcggcgga ctggcggtca
      121 tcccgcagga catccccacc gacgtggtcg ccgacgtggt ccagtgggta aagtcgcgcc
      181 acctggtgca cgacacggcg ctcacgctca agccgaccga cacggtcggt gatgcgctca
      241 acctgctgcc caagcgggcg cacggcgcgg tcatcgtcgt ggacgtggac cactgcccgg
      301 tcggggtggt gaccgaggcc gactgcctgg gcgtcgaccg gttcacccag ctgcgccagg
      361 tgatgtcggt cgagctgctc accctcccgg tcgaggtcga cccgcagcac gcgttcgagc
      421 gtctgcaggg cggacgccac cggcttgccc cggtcgtcga cgaggcgggc cggctggtcg
      481 gcctgctcac ccggaccggt gcgctgcggg ccaccctcta ctcccctgcc cttgactcgg
      541 ccggccggct ccgggtggcc gccgcggtcg gcatcaacgg cgacgtgacg gccaaggcgt
      601 cggcgctgct cgaggcgggg gtcgacctgc tggtcatcga caccgcgcac gggcaccagg
      661 agaagatgct ggccgtcctc gaggcggtgc gcgcactggc ccccgccgta cccctggtcg
      721 ctggcaacgt cgtctcggcc gagggggtcc gcgacctggt cgaggccggc gccgacatcg
      781 tcaaggtcgg ggtggggccg ggcgcgatgt gcaccacacg gatgatgacc ggggtggggc
      841 gtccccagtt ctccgccgtg ctcgagtgcg ccgccgaggc cgcccggctc ggccggcacg
      901 tgtgggcgga cggcggcgtg cgccacccgc gcgacgtcgc cctcgcgctg gcggccggtg
      961 cgtccaacgt gatgatcggg tcctggttcg ccggcaccca cgagtcgccc ggcgacgccc
     1021 atcgcgaccc cgacggccgg ctctacaagg agagcttcgg gatggcctcc gcgcgcgctg
     1081 tcgccctgcg cacccaggag gactcggtgt tcgagcgggc gcgcaaggcg ctgttcgagg
     1141 agggcatctc gtcggcccgg atgtacgtcg accccgagcg gcccggtgtc gaggacctgc
     1201 tcgacgcgat cgtcgccggt ctgcgcagcg cctgcaccta cgcgggcgcc gacacgctgg
     1261 cccagctgca cgagcgggcg gtggtcggcg tgcagagcgc cgccggctac gccgagggcc
     1321 ggccgctgca cgtcagctgg tgacgaggcg gtgcccgagc ccctgcgggt gacccgcgcc
     1381 gtggcgatcc ctgccggaga gctgcgctgg cggttctcgc gctcgagcgg gccaggcggg
     1441 cagtcggtca acacgaccga ctcgcgggcc gagctctcgt tcgacgtcgc cgggtcgccg
     1501 acgttgtcac cggcgctgcg ggctcgggcg ctgcagcggc tggccggccg gctggccgac
     1561 ggggtgctga ccgtggtggc cgccgagcac cgctcccagc tgcgcaaccg cgacgcggcc
     1621 ctggcccgga tggcggcgct gctggcgcag gcgatcgcgc cgccgccgcg gcagcgacgg
     1681 gccacccgcc cgacccgcgg gtccgtggag cgccggctgg ccagcaagca ccggcgcagc
     1741 gagctcaaac gctcacgccg gccggacccc gactgagcgt cgaggatcga cccggctagg
     1801 gcaggcgcag gtgggtgccg acccgcagga agttggggtt ggtgccgatg gtgtcgcggt
     1861 tggcctgcca gagcgcgcgc cagccgccgc tcacgtgggc acgtgccgcg atccgcgaga
     1921 gcgtgtcacc ggtgcgcacc gtgtaggtgc cctgggtggt cgacggcgcg ggggtgcgcg
     1981 ccggcgggct ggccttcgcc ggagcgggcg tcgacgcggc ccggccgggg acgacgagcg
     2041 aggtgcccac ccgcagcagg ttggggttgg cgccgatcag cgcctggttg gccttccaca
     2101 gcgccttcca gccgcccggg gcgcgcagct tggccgcgat gcccgacagc gtgtcgccgc
     2161 tgcggacggt gtaggtgccg cgcaccgtgg aggtcgcggc gggcggcgcc gtgcgggcga
     2221 gcaccggggc acggtcggcg gcccgggagg cgcgggggac gcgcgcgtcg tcggcgccca
     2281 gtcccagctt ggccgagcag gccggccagg cgccccagcc ctgcaccgcc agcactcgct
     2341 cggcgacggc gatctgctgc tcgcggctgg ccaggtcggc gcgtgcggcg taggcggtgc
     2401 cgccgaagct gcgccaggtc gcgggccaga actgcagccc cccgtagtag ccgttgccgg
     2461 tgttgacctg ccaacggccg ctcgactcgc acatcgccag ccggtcccac gtggtgtcgg
     2521 tggccgcctg cgcgggtgcc acggacgcca cgaccgggac ggccgagacc gctgcggtgg
     2581 cggcggcgac cacgaggcgc ccgtgcgacg gtgacggcgt gcggtgcttc ggctggtagc
     2641 gcatggctgt gtcgacgcct cccctgtgga aggcaatcga ccttccgtga tcacggatcg
     2701 gtcacgagag tacggagtag gacacctcgg ggcaaccctg cgaagtctcg gtgcgaagtc
     2761 atgcgagcgg ctgcccccgg tgacaggctc ccttagtggc gcgcatgccg gaccgggcgg
     2821 gggcgtggaa gaccgccggt ctcggcgtgg ggcttcccct cctgctcgcc ctgctggtgc
     2881 tcagcctcga cctcgtcgaa ggacccgagc ctgatttctt cggcgtgctg gtggctccgg
     2941 cattcctggc cgcggcgctc gtcgggccgg cgtggacagc actcaccgcc gccgccacgg
     3001 cggggatcgc gttcgggttc gggttcctgc agaagaacga cacgctcacc gcgagcgcgg
     3061 cgttcacaag tgcccagtgg acccggctgc tgttcatcct ggtgtcaggg atcctcgccg
     3121 tggtggtcgc gaccgtgcgc gtgcagcggg aggctcgact ggcgcggttc atgcagatca
     3181 gcgaggtcgc ccagcgtgcg atcctgcccg agctgccccg ccgagtcggc ggcctgccct
     3241 gcgccggtcg ctaccagtcg gccacgagtg aggctcacgt cggcggtgat ctctacgagg
     3301 tgctcgacac cgcgtacggc gtacgcgccc tgatcggcga cgcccgcggg aaggggatcg
     3361 acgcggtccg ggtggctggc tacgtgctcg ggtgcttccg cgcggtcgcg tggtcagagc
     3421 ccgacctcgc gcggctggcc cgctcgctcg acagcgccgt acgccggatc ggcggtcccg
     3481 aggacttcgt gactgccgct ctggtgcagc tcgacgggaa agacgatgcc cggctggtcg
     3541 tgtgcggaca tccccctccg ttgagcatcc ctccgccgcc catcgctggt ggccagtcgc
     3601 ccgcccagcc cgtcacatgt gatctgcccg tgacgcccct gctcgccagc gaacccgacc
     3661 cgccgctcgg cctgctccat gcgccgcccc gggtccaccg gtgcgccatc gctcccggcg
     3721 accggatcct gctcatcacc gacggcctcg ctgaggctcg ctggcgaggg cggttcctcg
     3781 atgtcgaggg gacggtggcg accgcctttc ggaccaccag tctcgacacc gcactggacg
     3841 cgatcctgac gacggcccgc cggcacgtgc acggcctggt ggccgatgac ctggcgctcg
     3901 tcgcgatcca gaccgccgcc tgaccgccgt cgtggtaacc ggccccgcgg ctcgggtcag
     3961 ctgactcgtt cggcgagccg agcgcgctgc ttctcccggc gctcctcgaa ccgcgaggcc
     4021 ttgatgtcga gctcctcgag aaaggcggcc agccgctcgc gggcggcctc ggcctcgccg
     4081 gacagccctt cgacgtccca gaccttccac ttgcgcagga tcggcgccag cacctcgtcg
     4141 cggtggatgc gcaggtcgta gatgccggcc tgggcgatct ggatcgagcg acgggtgaag
     4201 ttggccagcc cggcaccggg catcgcgaac gtgttgacga cgtcccagac cgccttcatg
     4261 gtctggtcgg gggcgacctc gaaggctccc gtgagcaggt tgcggtagaa gagcatgtgc
     4321 aggttctcgt cggccgcaat cttggcgagc agcgcgtcgg cgaccgggtc ctcggtgaac
     4381 ttgccggtgt tgcggtgcga gatgcgggtg gccagctcct ggaacgacac gtaggccacc
     4441 gtgtgcagca tgtcgagctc gttggcgctg ttgtagccct cggtcatgtg ggccatccgc
     4501 tcgcgctcga gctcaaccgg gtcgaccgcc cgagtggtca gcaggtagtc gcgcagcgca
     4561 atgccgtggc ggccctcctc ggcggtccag cgaccgaccc aggtccccca cgcgccgtcc
     4621 gggccgaaga cccggtagat ctcgcggtgg tagctcggca ggttgtcctc ggtgagcagg
     4681 ttgaccacga gcgcggtctg cgccacctcg gagaccttgg cctgcatggg gtcccacgcc
     4741 tcaccgtcga gggggccgtc gtagtcacgc ccctcgctcc acgggatgta ggcgtgcggg
     4801 aaccactcct tggaggtggt caggtgccgg ttgagctctt tctccacgac cggctcgagc
     4861 gcgtgcaaca gctgggtctg ggtcaactcg gccacgacgg gtcctcctcg ggcgttacct
     4921 tcggcaccgt aagttacggt tacggtaccg taggttcgtt accaggatag cgggtccccc
     4981 cggcgcggca agaccccgcg ggccagcatt caggcgccag acgcagtacg gcgacggcgc
     5041 gcggcgctcg acctgaccgc cttggccagg gtgtcggccg ccagcagcac cgggatcgcg
     5101 gtcgcggcga gcgcccagcc gagcgccggc ggccaggcgc caccgagcag gctcgccacc
     5161 ggatcgacgg tgaggaagcc gaccagcagg acgagctcga tgccgaccgc caccagcagc
     5221 aacgggttgc cccgcagccc cttctgcccc acccagctgg tctcgctgcg acaggcgaag
     5281 gcgttggcca gctggccgag cacgacggcg gcgaacgcgg cgccggaggc cccggccagc
     5341 agcgactcgc tcggcgtggc cgcccagacc cagccgccgg cgagcaggac cgcgacgaac
     5401 gccgccatct cgaccacggc ttcggccggc ccgagcaccc cgaacacccg gcgcagaagc
     5461 cgggcatcga tgagcgcgcc ggaccgggcc gggccctgca ggacccgagg gctgggtggc
     5521 tcgcccccca gggccagggc cggcagcagg tcggtgccga tgtcgagggc gagcacctgc
     5581 agcaccccca gcgccagcgg gacctggccg ccggacaggg cccagaccac gaacggggtg
     5641 agctcagcga cgttgtcggt caggtggtag gtgagaaacc ggcgaatgtt ggtgaacgtc
     5701 gcccgcccgg cggccaccgc gacgacgatc gtggcgaggt ggtcatcgag caggacgagg
     5761 tcggcagcct cacgcgccac gtcggtgccc gacccgccca tcgccacgcc gatgtcggcg
     5821 gcccgcagcg ccggcccgtc gttgacgccg tccccggtca tcgcgacgac gtgcccacgg
     5881 ccctgcaggg cgcgggcgat ccgctgcttg tcctgcgggg cgacccgggc cacgacgacg
     5941 ccgtcccggt cgagcagctc ccccagcatc tggtcgtcct cgggcagatc ggcaccggtg
     6001 accacgagct cgtcgggccc gagcaggccg gtctcccggg cgatggcgcg cgcggtctcc
     6061 ggagcgtctc cggtcaccat ggcgacgcgg atggccgcct gccgcaggtc ggccagcgcc
     6121 tcggccacgc cctcgcgggg cgggtcctcg atgccgatca gggccagcag ctcgagatcg
     6181 cactcggccg tttcgacgtc ggccgggagg gcctcgtcgg cgagccgtct cgcggcgacg
     6241 gcgaggaccc gcaacccgcg gtggcctagg ccggcgaccg cggcagcggc actcgggggt
     6301 acgtcgaggc agcgcggcag cacggcctcg ggggccccct tgagcaggac ggcgtcggtg
     6361 gtgaccagcg acatccgccg gcggcgtggg tcgaacggga accgtcggcg ggcgggccgg
     6421 gcccgctcgt cggcctcgac gtcggcgccg gcccgcagcg ccagcacgtg gagggcgacc
     6481 tccatcgggt cgccctgcgg ctgccactgc tccccccgcc gtacgacccg cccggtggag
     6541 cagcgtgccg cgaccagtgc cgccgcacgt atcgcctcta ccgcggtcgg cgacccggct
     6601 agccgaccca ccggcgtgta gccgccgccc tcgacccgga ccgacccgtg ccgggtccag
     6661 gcctcgacca cgctcatctc gttgcgcgtg agcgtgccgg tcttgtcggt gcacacgaag
     6721 gtggtggcgc ccagggtttc gaccgcctcc aggtgacgca cgagagcgtg ctgggcggcc
     6781 atccgctgcg cggcccgcgc cagcgagagc gtcacggttg gcagcagccc ctcggggacc
     6841 agcgccacgg tgaccccgac cgcgaacagg aacccgtctt gagcggggct gcccagcgcc
     6901 agcgccagcc cgaagaaccc tgcgcccgtg gccacggcca gcacggccac cacccacacc
     6961 acccggtgca gccgccgggc cagcgggctg accggccgat ggccctcgcg ggtcagggcg
     7021 gcgatctcgg ccagccgggt gcgtgacccg gtggcctcca ccactgcctc ggcttcgccc
     7081 tcgaccacga acgtgcccgc gaagacccga tggtccacgt cgcgcggcac cggcacgctc
     7141 tcgccggtga gcatcgactc gtcgaccgcc agcccgtcgc tgaccatcag ccgcaggtcg
     7201 gcgctgatcc ggtccccggc ggagagcagc acgaggtcgc ccacgaccag ctcggccgcg
     7261 gccacgacgg cgcgtcgcgc gtcacgccgt acgacgaccc gggccggcag catctcctgc
     7321 agccgccgcc cggcctggtc ggcccgccac tcctgggcga aggcgaacac cccgttgagc
     7381 agcaccacga tcacgatcgc gacgccgagc tgcggcagcc cggcgagcac cgccagcccg
     7441 gcggccaccc agagcatgag cgcgaagaag tgggtcagct gggcggccag cgccctggcg
     7501 gtggagcgcg gccgcgctcg cggcaggacg ttggggccgt cgcggcggag ccggaccgcc
     7561 gcctccgact gcgtcagccc ccggcccggc accccggcat ccggtcgagc gggaacgctt
     7621 gccatgtgcc ccccgacgca ggctgtaccg ccgggcgccg ggcccggcag gggcgaaggt
     7681 ccccggccac gatagggtgg gtctcatcag cggcactctg agtcgctgcc tcccgcgggc
     7741 gagtcgcccg cgcggtcgct cgagtgagca gctggatcct ccggcgcggc gttccgtgcc
     7801 tgttccatca cgggttcatc gcttcgcgtc agcgcggcca tcgacccatg ttcggagcac
     7861 gcacgcccat gtccatcgac acctccatgt ctactgatcg ccgcacgcct gcggcggcgc
     7921 ccagcttcgc cgcgctgggc ctgccccaac gcctggtcac cgccctcgag cgaaatgaca
     7981 tccgcgcgcc gttcgcgatc cagacgctgg ccctgcccga tgcgctcgcc gggcgggacg
     8041 tcctcggccg ggcccggacc ggttcgggca agacgctggc cttcggcctg cccctgctcg
     8101 cccggctgag ctcgtcgcac acccccgggc gggcgccgcg cgccctcgtg ctggtgccca
     8161 cccgcgagct ggccgtgcag gtgcgcgacg tgctcgagcc gctcgggcac gcggtcgggc
     8221 tcaaggtggt cgccatcttc ggcggcgcct cgatgggccg tcagcgtcag gcgctgcagc
     8281 gcgggctaga cgtgatcgtc gctacaccgg gccgcttgca ggacttcttc gagcgcggtg
     8341 acgcccgcct cgacgagatc gaggtgagcg tgctcgacga ggccgacttc atggccgacc
     8401 tgggattcct cccggtcgtc tcacgactgc tcgacgccac ccccgcgcac ggtcagcgac
     8461 tgctgttctc cgccacgctc gacggcgcgg tcaccacgat cgtgcgccgc tacctcacca
     8521 acccggtcac ccactcggtc gagcccgctg ccgcctcgac cggctcgatc gaccaccggg
     8581 cctacatcgt ctccgccacc gacaaggtcg ccgtgctcgc cgcgatcgcg tcccggccgg
     8641 cacgcaccct gatgttcgtg cgcaccaagc acggggccga ccggctggcc acccagctcg
     8701 gccgactcgg ggtcgaggcc accgcgatcc acggcaacct caaccagaac cagcgccagc
     8761 gggcactggc cgcattcacc tatggcgagt gccgggtgct ggtcgccacc gacgtggccg
     8821 cgcgcggcat ccacgtcgac gacgtcgacc tggtcgtgca cgtcgacccg cccagcgacc
     8881 acaaggacta cgtgcaccgg tcaggccgta cggcgcgcgc cggggccagc ggcaccgtgg
     8941 tgtcgatcct gctgcccgag caggtccgcc cgtccgagcg gctgcgtcag cgcgccggcg
     9001 tcaaggtcaa ggccatccgg gtcgaccccg gtcacccggc ggtgcgcgag ctggccacct
     9061 cgggtgagcc ggtcgtcgtc gtgccccgtc ccgcccccga acccgtcacc catcgcccgg
     9121 ccgggcgctc gcgccgaccg gcccgggcgg gctcgggcac cggcggacgc cggggctcgg
     9181 ccagcgccgc ggcgcgtccc gcctccgcgg gccgtccccg ccggcgccgg accgacaacc
     9241 cacgcagccc ccgcacggct gcctgactcc ggagtccatt ggtcccgttg ccgagggcgg
     9301 tgcacctgct tagcgtcacg agcagggaca tcgcctcgga agggggacgc catgacggcc
     9361 acggcgaccg ccggcacgtc cagccacctg gacgcgtcgg cgcgcaagga gctctaccgg
     9421 cagatggtgc gcatccgcac cttcgaggaa gcgatcctgc gcgactacca cgccgacaag
     9481 aagcccgcct tcgacatcgg cgccgggctg atccccgggg agatgcacct ggccgccggc
     9541 caggagccgg tcgcggccgg gctgtgcgcg cacctgcgca ccgacgacgc ggtcaccgcg
     9601 acgcaccgcc cgcaccacct ggcgatcgcc cacgggatgg acctggccaa gctggccggg
     9661 gagatctatg gccgcgagac cgggctgggg cacggccgcg gcgggcacat gcacctcttt
     9721 gacccgagca cccacttctc atgctcgggg atcatcgccg agggcctccc acccgcgctg
     9781 gggcaggcct tcgcgttcaa gcgggccggc accgaccgcg tcgcggccgc cgtcaccgga
     9841 gagggggcgg ccaaccaggg cgggttccac gagtcgctca acctggccgc ccggtgggcg
     9901 ttgccggtgg tgttcgtggt cgaggacaac gactgggcca tctcggtgca ccgcgacacc
     9961 gcgaccgccg taccgaccaa cgccgaacgg gccgccgcct acgggatgcc cggcgagcgg
    10021 gtcgaggaca acgacgtcga ggcggtctac gacgcggccg agcgcgccgt gacccgggcc
    10081 cgacacggcg acgggccctc gctgatcgag gtgcacaccc tgcggctgtg gggccacttc
    10141 gagggcgacg cgcagggcta ccgacccgag ctggccgagg tgcccgagct cgacccgatc
    10201 ccccgctacg agcaggcact gcgcgccgcc ggtgtgctcg acgatgacga ggtcagccgc
    10261 atccacgccg aggccacccg ccaggtcggg gcggcggtgg acgccgcgaa ggcggcgccg
    10321 ctgcctgacc ccgccaccgc gacgtcctac gtcttcgccg acgcctgagg aggttcccgt
    10381 gaccgtcacc agcagtgcca cgatccccgg acagacctcc gggcgccgtc tcacgaccgc
    10441 caaggcgatg gtggaggcca tcgccgcgga gatggagcgc gacccgtcgg tggtctacct
    10501 cggcgaggac gtcggggcct acggcggcat cttcagctcg acgaccgggc tgctcgaccg
    10561 gttcggcccc gaccgagtga tcgacacccc gatctccgag acagccttca tggggctggc
    10621 cacgggggcc gccgtcgagg ggatgcgccc gatcgtcgag ctgatgttcg ccgacttctt
    10681 cggggtgtgc ttcgaccaga tctacaacca catggccaag atccacttcg agtccggcgg
    10741 caacgtgaag gtgccgctcg tgctgaccac cgcggtcggc ggc
//
LOCUS       DATIBB010000046         7508 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_546924, whole genome shotgun sequence.
ACCESSION   DATIBB010000046 DATIBB010000000
VERSION     DATIBB010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 7508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7508
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_546924"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1023)
                     /locus_tag="VLC50_01605"
     CDS             complement(<1..1023)
                     /locus_tag="VLC50_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004020800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HSF26190.1"
                     /translation="MTTLDTATTPRRYGHFIAGDWRESDETIARYAPGTGALVTEVAA
                     GDAPEVDAAVRAAREAFDSGPWPRATGFERAALLHKVADLIDANAELLARLDAEEGGK
                     PIKLAEGDIAGAATLTRYAAGLAVQMHGSTYTNHGPDFTGLVLREPAGVVGLITPWNF
                     PALILCQKLPFALAAGCTVVAKPSEFTSSTTLEIARLYAEAGLPEGVLNVVTGDGRAG
                     GALAEHHGVDVISFTGSTATGRKILDASKGNLKKLSLELGGKAANIVFADADLEDAAE
                     GVTFGVFFNNGECCVSQARLLVQDSVADDFLAQLATAAGRLRVGQPLDHATDVGAMIH
                     PGHIEKV"
     gene            complement(1052..2188)
                     /locus_tag="VLC50_01610"
     CDS             complement(1052..2188)
                     /locus_tag="VLC50_01610"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HSF26191.1"
                     /translation="MAQSFSSPPRVLLGDDALSQLAATLPRLGTKVLVVTDPGVKAAG
                     LLDQVTAALASSVPVTVFAEVAGNPDVDTVDAAAAAGKDFGADIVLGLGGGSSLDVAK
                     SVAILATNGGSIRDYEGVDRFATRPLPVVAVPTTVGSGSEVTKGAVISDPATLVKMVI
                     VSDLMFPAVAVLAPSLVASLPGRIAATTGMDALTHAIEAYVAKGANPITDVINIGAIE
                     LIGRSIVAAAQGDADALYDMLVASCMAGIGFHDAGLGAVHALANTLGGHFGVHHGTAN
                     ALFLPYVMDFNLEAAPAKFARIAVALGEDVTGLDDLAAARRAAAAVHRLAEQTGVPRK
                     LADVGVPETAIDALAADALVQADLPGNPRPCTLDDLIALYRAAL"
     gene            complement(2240..3094)
                     /locus_tag="VLC50_01615"
     CDS             complement(2240..3094)
                     /locus_tag="VLC50_01615"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HSF26192.1"
                     /translation="METADITMVGVDTLAVGQGPAAAERRGPRRVQAVEHAIDVLEAM
                     AKAARPIGVSDLARRVGLSKATVHHLLATLEGRHFVMQDPHSSLYRLSWALYELGSSV
                     VRDVELSRVARPYLDRLAMKSGESVLLGILDGSSVLYLDRGEAQGGLRMVASAGRRGS
                     LHATASGKVLLAFSPDPALVENVLSNPLEKMTRTTVTEPATLRRQLTQIRSRGYATCW
                     QEREVGLCSVAVPLRDYTGQVVGSLALAGPASRLTPRTLQTHLSQLTHTAHDIELHIG
                     GARAPVEA"
     gene            3351..4436
                     /locus_tag="VLC50_01620"
     CDS             3351..4436
                     /locus_tag="VLC50_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020387621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HSF26193.1"
                     /translation="MVALGLAAALSLTACGSGSSSSSDEPGATAAPTNGAAQADAYKI
                     GLLLPENLVPRYEGKDKPYFEEKLKELCPDCEMLYANADADVAKQKQQADSMLAQGVN
                     VLVVDPLDGVAAASIVAAAKAANVPVVAYDRLIKSPDLSFVISNDYKQVGVLQAQALV
                     DKLKADGVDPAKGGILMMNGATTDNNAGNIKAGALSIIEPSGYKILASIDTWDPAEAQ
                     QWVSGQITKFRDQIVAVYSANDGNAGGAIAALKAAGVKTIPPITGLDASLQGLQAILA
                     GDQYMTTYNAFRSEAHKAAEVALALAKGETPASDNEVEGIPAALNPPQAVTVDTIAST
                     VIADEFFTLDEICTAQYKAACDAAGIK"
     gene            4539..5363
                     /locus_tag="VLC50_01625"
     CDS             4539..5363
                     /locus_tag="VLC50_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010069797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HSF26194.1"
                     /translation="MSLTETATPPQANGQPLLSLRGVSKAFGVIQALSDIDLDIFAGE
                     VVALLGDNGAGKSTLVKILAGVHPADSGSIAFKGQPVSISSPMESRNLGIATVYQDLA
                     LCENLDVVANLFLGREEGRFTLAEESMEKRTWSLLRQLSAKIPSVRTPVAALSGGQRQ
                     TVAIARSLIGEPSIVMLDEPTAALGVEQTAEVLNLIERLRDRGHGVLLITHNMANVKS
                     VCDRAAVLRLGRNNGTFRVAEVSTEDIVAAITGATDSAVTRRAQATAEKATKVTSA"
     gene            5360..6619
                     /locus_tag="VLC50_01630"
     CDS             5360..6619
                     /locus_tag="VLC50_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020387623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HSF26195.1"
                     /translation="MSGTSAPAVEPGLPTDLQDERLIRRTGPRGAAAAMWMRIRTGDI
                     GSLPVVLGLVIIWAVFQSLNPYFLSSANLVNLAIECAAVGTIAIGVVLVLLVAQIDLS
                     VGSMSGVASSILGVGLAKLGWSTPMAVVVALVAGVVVGLVYGFIYVRFGVPTFVITLA
                     GLLALLGLQLQILGTNGSINIPFESWIVRFNQQMYLPAVWSYAISVVAALAFASSGLA
                     KARRRAAAGLSAASTTEVVLKAVAMLAVLEVFCWYLNKTRGIGAPFVLFVALVIIVDL
                     ALKRTRWGRSVYAVGGSVEGARRAGIKVNRVFVSVLVLGTTLAALGGLLAAGRLATAS
                     ISSGTGDVNLNAIAAAVIGGTSLFGGRGNAYSALLGIVVIASISSGLTLLSLDSSFRF
                     MITGTVLMIAVIVDSLSRRSRAAHGRA"
     gene            6661..>7508
                     /locus_tag="VLC50_01635"
     CDS             6661..>7508
                     /locus_tag="VLC50_01635"
                     /inference="COORDINATES: protein motif:HMM:NF015056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HSF26196.1"
                     /translation="MSTVHRLTAATVHSSWSGAHAPVLTVRSGDEVAFECRDGFDGQL
                     RGRASGPLGDNDLAGLDFGRIAPLTGPIRVEGARPGDGLSVQILDLAPSGPGWTVVWP
                     QWCGFDYHRPATVPPSGHLRVFDAETLRGGGSVELGPARVPVAPMLGMIGLAPAKGRF
                     PTQPPREFGGNLDLRLVGPGAIVRLPVLVDGANLSVGDGHAAQGDGEVCTTAIECAMT
                     GRLRVWVEPGAAPTQPEVETPEAYITTGYGRTLDDAVRMAIDGMHGWLCRRGLNPVDA
                     YMLLSIA"
BASE COUNT         1098 a         2640 c         2661 g         1109 t
ORIGIN      
        1 gaccttctcg atgtggccgg ggtggatcat cgcgccgacg tcggtggcgt ggtcgagcgg
       61 ctggccgacc cgcagccggc cggcggccgt cgccagctgg gcgaggaagt cgtcggcgac
      121 gctgtcctgc acgagcagcc gagcctgcga cacgcagcac tcgccgttgt tgaagaacac
      181 gccgaaggtc acgccctcgg cggcgtcctc caggtcggcg tcggcgaaga cgatgttggc
      241 cgccttgccg ccgagctcga gggagagctt cttgaggttg cccttggagg cgtcgaggat
      301 cttgcggccg gtcgcggtgg agccggtgaa cgagatgacg tcgacgccgt ggtgctcggc
      361 gagcgcgcct ccggcccggc cgtcaccggt gaccacgttg agcacgcctt cgggcaggcc
      421 cgcctcggca tagagccggg cgatctccag cgtggtgctg ctggtgaact cggagggctt
      481 ggcgaccacg gtgcagccgg cggccagcgc gaagggcagc ttctggcaga ggatcagcgc
      541 cgggaagttc cacggcgtaa tgagcccgac gacgccggcc ggctcgcgca gcacgagccc
      601 ggtgaagtcg ggtccgtggt tggtgtaggt cgacccgtgc atctgcaccg ccagccccgc
      661 ggcatagcgg gtcagggtcg cggcgccggc gatgtcaccc tcggccagct tgatcggctt
      721 gccgccctcc tcggcgtcca gacgcgcgag cagctcggcg ttggcgtcga tgaggtcggc
      781 gaccttgtgc agcagggcgg cccgctcgaa gccggtggcc cgcggccagg gccccgagtc
      841 aaaggcctcc cgggcggcgc gtacggcggc gtcgacctcg ggcgcgtcgc cggccgcgac
      901 ctcggtgacc agcgcgccgg tgccgggggc gtagcgggcg atggtctcgt cggactcacg
      961 ccagtcaccg gcgatgaagt gcccgtagcg gcgtggggtg gtcgcggtgt cgagggtggt
     1021 caacggaaat cctccagagt gagggccggg ctcagagcgc ggcgcggtag agcgcgatca
     1081 ggtcgtcgag cgtgcagggg cgagggttgc cagggaggtc ggcctgcacc agcgcgtcgg
     1141 cggccagtgc gtcgatcgcg gtctccggca cgccgacgtc ggcgagcttg cggggcaccc
     1201 cggtctgctc ggcgagccgg tgcaccgcgg ccgcggcgcg gcgggcagcc gcaaggtcgt
     1261 cgagcccggt cacgtcctca ccgagcgcga cggcgatgcg ggcgaacttg gcgggggccg
     1321 cctcgaggtt gaagtccatc acgtagggaa ggaacagagc attcgccgta ccgtggtgca
     1381 ccccgaagtg tccgcccagc gtgttggcca gggcgtgcac ggcgccgagc cctgcgtcgt
     1441 ggaagccgat gccggccatg cacgaggcca ccagcatgtc gtagagcgcg tccgcgtcac
     1501 cctgggcggc tgccacgatg ctgcgcccga tgagctcgat cgccccgatg ttgatgacgt
     1561 cggtgatcgg gttggcgccc ttggcgacgt aggcctcgat ggcgtgggtg agcgcgtcca
     1621 tgccggtggt ggcggcgatc cgaccgggca gcgaggcgac gagcgagggc gcgagcaccg
     1681 cgacggccgg gaacatcagg tcggagacga tgaccatctt gaccaaggtg gcggggtcgg
     1741 agatgaccgc gcccttggtg acctcgctgc cgctgccgac ggtcgtggga accgcgacga
     1801 ccggcagcgg gcgggtggcg aaccggtcga cgccctcgta gtcgcggatg ctcccgccgt
     1861 tggtggcgag gatggccacc gacttggcca cgtcgaggct gctgcccccg cccagcccga
     1921 gcacgatgtc ggcgccgaag tccttgccgg ccgccgcggc cgcgtcgacg gtgtcgacgt
     1981 cagggttgcc ggccacctca gcgaagacgg tgaccggcac cgaagaggcc agcgccgccg
     2041 tcacctggtc gagcaggccg gccgccttga ccccggggtc ggtgacgacc aagaccttgg
     2101 tgcccagccg gggcagggtc gcggcgagct ggctgagcgc gtcgtcgccg agcaggacgc
     2161 gcggcggcga gctgaacgat tgggccatcg agggggtctc ctgtgcaggt gcgctggtgc
     2221 gggacggacg ggcggggggt caggcttcca ccggggcgcg ggcgccgccg atgtgcagct
     2281 cgatgtcgtg cgcggtgtgg gtgagctggg acaggtgggt ctgcagcgtg cgcggggtga
     2341 gccgggaagc cggcccggcc agcgcgagcg agcccacgac ctgaccggtg tagtcgcgca
     2401 gcggtacggc gaccgagcac agccccacct cacgctcctg ccagcaggtc gcgtagccgc
     2461 gggagcggat ctgcgtcagc tgacggcgca aggtggccgg ctcggtcacc gtggtccggg
     2521 tcatcttctc cagcgggttg ctcagcacgt tctcgaccag ggccgggtcg gggctgaagg
     2581 ccagcagcac cttgcccgac gcggtggcgt gcagggagcc gcgccgacca gcgctggcca
     2641 ccatccgcag gccaccctgg gcctccccac ggtcgagata gagcaccgag ctgccgtcga
     2701 ggatgcccag cagcaccgac tcgccggact tcatggccag ccggtcgagg tagggccgcg
     2761 ccacgcgaga cagctcgacg tcgcgcacca cgctggagcc gagctcatag agcgcccagc
     2821 tgagccggta gagcgacgag tgcgggtcct gcatgacgaa gtggcggccc tcgagggtgg
     2881 ccagcaggtg gtggacggtc gccttgctca gcccgacccg gcgggccagg tcgctgaccc
     2941 cgatcgggcg agccgccttg gccatggcct caaggacgtc gatggcgtgc tcgacggcct
     3001 ggacccgtcg gggcccacgc cgttctgctg ccgccggtcc ctgacctacc gccaacgtgt
     3061 ccactcccac catcgtgatg tccgccgtct ccacgcgtcg aggggcacct gctgcgaggg
     3121 ccaatcggcg tttccagggc gtggcatcaa ccctaaggtt ccgcgaacac cgggtctatc
     3181 accgcttgca aaaagtgttt ggaattgcca aaccgtgttc accacttgtt gcggcggacc
     3241 tatacattcg ccgcagtcac cgcccaacgg cactcgagtc cacgcacgca ggacccgttg
     3301 gcacacccct tggaaggcag ggttgtgaaa tgcgcagaag cgcagcaagc ttggtcgccc
     3361 tcggcctcgc ggccgcactc tcactgacgg cctgcggctc cggttccagc tccagctcgg
     3421 acgagccggg agcgaccgca gcgcccacca acggcgcggc gcaggccgac gcctacaaga
     3481 tcgggctgct cctcccggag aacctcgtcc cgcgctacga gggcaaggac aagccctact
     3541 tcgaggagaa gctcaaggag ctctgccccg actgcgagat gctctacgcc aacgctgacg
     3601 ccgacgtggc caagcagaag cagcaggctg actcgatgct cgcgcagggc gtcaacgtcc
     3661 tggtcgtcga cccgctcgac ggcgttgccg cggcgagcat cgtggccgcc gcgaaggcag
     3721 ccaacgtgcc cgtggtcgcc tacgaccgcc tgatcaagag cccggacctg tcgttcgtca
     3781 tctccaacga ctacaagcag gtcggcgtcc tgcaggccca ggcgctggtc gacaagctca
     3841 aggccgacgg ggtcgacccg gccaagggcg gcatcctcat gatgaatggc gcgacgaccg
     3901 acaacaacgc cggcaacatc aaggccggtg cgctgagcat catcgagccg agcggctaca
     3961 agatcctcgc gtcgatcgac acgtgggacc cggccgaggc ccagcagtgg gtgtccggcc
     4021 agatcaccaa gttccgtgac cagatcgtcg cggtctactc ggccaacgac ggcaacgcag
     4081 gcggcgccat cgcggcgctg aaggcagccg gcgtgaagac gatcccgccg atcaccggtc
     4141 tggacgcctc gctgcagggc ctgcaggcca tcctggccgg cgaccagtac atgacgacct
     4201 acaacgcctt ccggtccgag gcccacaagg ccgccgaggt cgcgttggcc ctggccaagg
     4261 gcgagacccc ggccagcgac aacgaggtcg aggggatccc ggcggccctc aacccgccgc
     4321 aggccgtcac cgtcgacacg attgcctcga cggtcatcgc cgacgagttc ttcactctcg
     4381 acgagatctg caccgcgcag tacaaggcag cctgcgacgc tgctgggatc aagtagcttt
     4441 cccaactcgc agccggctgg aggcccagcg cacactggta tccctccagc cggctgcgta
     4501 ctctcgtccg gaaaaccacc ccacccgagg actcccacat gtcgttgacg gagaccgcga
     4561 cgccgcccca ggcaaacgga cagcccttgc tgtcgcttcg tggcgtgagc aaagcattcg
     4621 gcgtcatcca ggctctgtct gacatcgacc tcgacatctt tgccggtgaa gtggtcgcac
     4681 tgctcggcga caatggcgcc ggaaagtcga ccctggtcaa gattctggcc ggcgtacatc
     4741 ccgccgactc cgggtcgatc gcgttcaagg ggcaaccggt ctccatctcc agccccatgg
     4801 agtcgcgcaa cctcggcatc gcgaccgtct accaggacct cgcgctgtgc gagaacctcg
     4861 acgtcgtcgc caacctcttc ctcggccgcg aggaaggacg cttcaccctc gccgaagagt
     4921 cgatggagaa gcggacctgg tcgctgctgc gccagctctc ggccaagatc ccctccgtgc
     4981 gcaccccggt cgccgcgctc tccggtggcc agcgtcagac ggtcgcgatc gcccgctcgc
     5041 tcatcggcga gccctcgatc gtcatgctcg acgagccgac cgccgcgctc ggggtcgagc
     5101 agaccgccga ggtgctcaac ctcatcgaac ggctgcgcga ccgcgggcac ggggtcctgc
     5161 tcatcaccca caacatggcc aacgtgaagt ccgtctgcga ccgggccgcc gtgctgcggc
     5221 tgggccgcaa caacgggacg ttccgggtcg ccgaggtgtc gaccgaggac atcgtcgcgg
     5281 cgatcacggg ggccaccgac agcgccgtca cgagacgggc tcaggcgacc gcggagaagg
     5341 cgacgaaggt gacctcagca tgagcggcac gagcgccccg gccgtcgagc cgggcctgcc
     5401 caccgacctg caggacgagc ggctcatccg caggaccggt ccccgcgggg ccgccgccgc
     5461 gatgtggatg cgcatccgca ccggcgacat cggctcgctg ccggtggtgc tgggcctggt
     5521 catcatctgg gcggtcttcc agtcgctcaa cccctacttc ctctccagcg ccaacctggt
     5581 caacctggcc atcgagtgcg ccgcggtcgg cacgatcgcg atcggcgtgg tcctcgtgct
     5641 gctcgtcgcc cagatcgacc tgtcggtcgg ctcgatgagc ggggtggcct cctcgatcct
     5701 cggcgtgggc ctggccaagc tcggctggtc gacacccatg gcggtcgtcg tcgccctcgt
     5761 cgccggcgtc gtggtcggcc tggtctacgg gttcatctac gtgcgcttcg gcgtaccgac
     5821 gtttgtcatc acgctggccg gcctgctcgc gctgctcggc ctgcagctgc agatcctcgg
     5881 caccaacggc tcgatcaaca tcccgttcga gtcgtggatc gtgcggttca accagcagat
     5941 gtacctgccg gccgtctggt cctacgcgat ctccgtggtc gccgcgctgg ccttcgccag
     6001 ctcggggctg gccaaggcgc gccggcgtgc ggccgcgggc ctgtcggcgg cctcgacgac
     6061 cgaggtcgtc ctcaaggccg tcgcgatgct cgcggttctc gaggtgttct gctggtacct
     6121 caacaagacc cgcggcatcg gtgcgccgtt cgtgctgttc gtcgccctgg tgattatcgt
     6181 cgacctcgcg ctcaagcgga cccgctgggg ccggtcggtc tatgccgtcg gtggctcggt
     6241 cgagggcgcc cggcgcgccg gcatcaaggt caaccgggtc ttcgtgtcgg tgctcgtgct
     6301 gggcaccacg ctcgccgcgc tcggtggact gctcgccgcc ggacggctgg ccaccgcgag
     6361 catcagcagc ggcaccggcg acgtcaacct caacgcgatc gcggcggcgg tcatcggcgg
     6421 caccagcctg ttcggcggcc gcggcaacgc ctactcggcg ctgctgggca tcgtcgtgat
     6481 cgcctcgatc tccagcgggc tgaccctgct cagcctcgac tcgtcgttcc gattcatgat
     6541 caccggcacc gtgctgatga tcgcggtcat cgtcgactcg ctgtcgcgtc ggtcccgggc
     6601 ggcgcacggc cgggcctagc gccggcccgc tgagctcgac gcccccgccg cggtgagctg
     6661 atgagcaccg tccaccggct caccgccgcc acggtgcact cgagctggtc gggcgcgcac
     6721 gcaccggtgc tcaccgtgcg cagcggcgac gaggtggcct tcgagtgtcg tgacggcttc
     6781 gacggtcagc tgcgcggccg ggcctccggc ccgctcggcg acaacgacct ggccgggctc
     6841 gacttcgggc ggatcgcgcc gctgaccggg ccgatccgcg tggagggcgc ccggcccggt
     6901 gacgggctga gcgtgcagat cctcgacctc gcgccctcgg gtcccggctg gaccgtggtc
     6961 tggccgcagt ggtgcggctt cgactaccac cggcccgcca cggtcccccc gagcggtcac
     7021 ctgcgggtct ttgatgcgga gacgttgcga ggcggcggct cggtcgagct cggccccgcc
     7081 cgggtgccgg tcgccccgat gctcggcatg atcggcctcg ccccggcgaa gggccggttc
     7141 ccgacccagc cgccacgcga gttcgggggc aacctcgacc tgcgcctggt cgggccgggc
     7201 gccatcgtgc gcctgccggt gctggtcgac ggggccaacc tgtccgtcgg cgacgggcac
     7261 gcggcccagg gcgacggtga ggtctgcacg acggcgatcg agtgcgccat gaccggacgg
     7321 ctacgggtgt gggtcgagcc cggtgccgcc cccacgcagc ccgaggtcga gacccccgag
     7381 gcctacatca ccaccggcta cggccgcacc ctcgacgacg cggtgcggat ggccatcgac
     7441 ggcatgcacg ggtggctgtg ccggcgcggt ctgaacccgg tcgacgctta catgctgctg
     7501 agcatcgc
//
LOCUS       DATIBB010000047         4531 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_1072784, whole genome shotgun sequence.
ACCESSION   DATIBB010000047 DATIBB010000000
VERSION     DATIBB010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 4531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4531
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1072784"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1323)
                     /gene="dnaE"
                     /locus_tag="VLC50_01640"
     CDS             complement(<1..1323)
                     /gene="dnaE"
                     /locus_tag="VLC50_01640"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015779605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HSF26197.1"
                     /translation="MGTPSDSFAHLHVHTEYSMLDGAARIDDLLAEAARMGMPAVATT
                     DHGFVFGAYDFWSKAAKHGIKPIIGVEAYLTPGTHRSDRTRVRWGDGGEDDVSGGGAY
                     THMTLLAETTQGMHNLFRMASLASLEGHFYKPRMDRELLSTYGRGLIATTGCPSGEVQ
                     TLLRLGRYDEARASAAEFRDIFGADNYFCELMDHGLGIQRRVQGDLLRLAADLGLPLV
                     ATNDLHYTRAEDAQAHAVLLCVQSGSTLADPKRFKFDADEFYLKSPEAMRHVWRELPE
                     ACDNTLRIAERCEVSFTESTGAYMPRFPCPDGEDETSWFIKEVERGLHDRFPDGIPDR
                     VRQQADYETEIIVNKGYPGYFLVVADFINWAKANGIRVGPGRGSGAGSLAAYAMRITD
                     LDPLQHGLIFERFLNPERMSMPDFDVDFDERRRGEVIRYVTERYGDDRV"
     gene            complement(1398..1877)
                     /locus_tag="VLC50_01645"
     CDS             complement(1398..1877)
                     /locus_tag="VLC50_01645"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010473439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HSF26198.1"
                     /translation="MSSPSLTVDVVSAVEGDSRWVEALDIRWEVFVVEQSVPAALERD
                     ERDPEADHVLARVDGVATGTGRLLVEPPGFAGVDPGCGPVAHIGRLAVRAGARGHGIG
                     AALVRALEAQAVARGLRVVYLGAQVHAVPFYERLGYTPYGEIFDDAGIEHRRMWRRV"
     gene            complement(1874..2803)
                     /locus_tag="VLC50_01650"
     CDS             complement(1874..2803)
                     /locus_tag="VLC50_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018837720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HSF26199.1"
                     /translation="MAEARILPVPEGLDGERVDAALARLFGFSRTQAAALAAAGAVVL
                     DQVVVGKSDRVHAGAVLEVEVPSPAAAPTAPPEPVPGLHVLYDDDDVVIVDKPIGVAA
                     HPSPGWTGPTVVGGLAAAGYRISTSGAPERQGVVHRLDVGTSGVMVVAKSERAYTVLK
                     RAFKARTVEKTYHAVVQGHLDPLRGTVDAPVGRHPTHDYRWAVVAGGKDSITHYDTLE
                     AFRAASLLEVHLQTGRTHQIRVHMSALHHPCVGDLTYGADPKLAARLGLGRQWLHAVR
                     LGLEHPSTGRWLQVDSPYPDDLAEALRRLRDAA"
     gene            complement(2796..3263)
                     /gene="lspA"
                     /locus_tag="VLC50_01655"
     CDS             complement(2796..3263)
                     /gene="lspA"
                     /locus_tag="VLC50_01655"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011435867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HSF26200.1"
                     /translation="MLVGVAVVVLALDQLTKLIAVHLLSDHEPVEVLGGLLTLRIVRN
                     SGAAFGLAGGATIEFTVVAAAVVIVILRIARRLVSLPWALSLGLLLGGAAGNLGDRLF
                     RGPAPLRGHVVDFLELPYWPVFNVADSAIVLAGITMVVYTLRGVAVDGTTSRG"
     gene            complement(3329..4150)
                     /locus_tag="VLC50_01660"
     CDS             complement(3329..4150)
                     /locus_tag="VLC50_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015661081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HSF26201.1"
                     /translation="MAARGTGPARAARATEPAQKNAAAPSAKKATPRTKAASTAKAAP
                     GAKPAKATKAASKTTRSASAARTATTARSKAPAKAATAAKPAATTSAAKPAAKATASR
                     SGATRAKAKPATGSTKKIPAQKSPASRSERARSASAVATTAARLAVREDESPWTPDEL
                     AQVRGDLESEVARLRSEITDAESVISDLLRDAGEGAGDDQADAGTKTFEREHEMSLAA
                     NSRDMLEQTLRALARIEAGTYGVCESCGNPIGKRRLQAFPRATLCLTCKQREERR"
     gene            complement(4259..>4531)
                     /locus_tag="VLC50_01665"
     CDS             complement(4259..>4531)
                     /locus_tag="VLC50_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007513746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HSF26202.1"
                     /translation="GASRTSVGGERAGALVVRVSARAVDGAATEAALRAVAEAFGVRR
                     RDVSLLRGATSRHKLVAIAAAAEVVTARCRQLKGAAGEEASGGGEH"
BASE COUNT          594 a         1646 c         1662 g          629 t
ORIGIN      
        1 gacccggtcg tcgccgtagc gctcggtgac gtagcggatg acctcaccgc ggcgacgctc
       61 gtcgaagtcg acgtcgaagt cgggcatcga catgcgctcg gggttgagga agcgctcgaa
      121 gatcagcccg tgctggagcg ggtcgaggtc ggtgatgcgc atggcgtagg ccgcgaggga
      181 cccggcaccc gacccacggc cggggccgac ccggatgccg ttggccttgg cccagttgat
      241 gaagtcggcc accacgagga agtaaccggg atagcccttg ttgacgatga tctcggtctc
      301 gtagtcggcc tgctggcgca cccggtcagg gatgccgtcg gggaagcggt cgtgcaggcc
      361 ccgctcgacc tccttgatga accacgaggt ctcgtcctcg ccgtcggggc acgggaagcg
      421 cggcatgtag gcgccggtgg actcggtgaa ggagacctcg caccgctcgg cgatgcgcag
      481 cgtgttgtcg caggcctccg gcagctcgcg ccacacgtgg cgcatcgcct cgggcgactt
      541 gaggtagaac tcgtcggcgt cgaacttgaa ccgcttgggg tcggccagcg tggagccgga
      601 ctgcacgcag agcagcaccg cgtgcgcctg cgcgtcttcg gcgcgggtgt agtgcaggtc
      661 gttggtcgcc accagcggca gcccgaggtc ggcggccagc cgcagcaggt caccctgcac
      721 ccgacgctgg atgcccaggc cgtggtccat cagctcgcag aagtagttgt cggcgccgaa
      781 gatgtcgcgg aactcggcgg ccgaggcccg cgcctcgtcg tagcggccca gccgcagcag
      841 ggtctggacc tctcccgatg ggcacccggt cgtggcgatg agaccgcggc cgtaggtcga
      901 gagcagctca cggtccatgc gcggcttgta gaagtgcccc tcgaggctgg ccagcgaggc
      961 catccggaac aggttgtgca tgccctgcgt ggtctcggcc agcagcgtca tgtgggtgta
     1021 ggccccgcca ccggagacgt cgtcctcgcc accgtcgccc caccggaccc gggtgcggtc
     1081 gctgcggtgg gtgcccgggg tgaggtaggc ctcgacgccg atgatcggct tgatgccgtg
     1141 cttggccgcc ttggaccaga agtcgtaggc cccgaagacg aacccgtggt cggtcgtagc
     1201 caccgccggc atgcccatgc gcgccgcctc ggcgagcagg tcgtcgatgc gggcggcgcc
     1261 gtcgagcatg gagtactcgg tgtgcacgtg caggtgggcg aacgagtcgc tcggcgtgcc
     1321 catgctccgc gaacctcctt gcctgtggcc acactcggcc agggggtgag cctaacggcg
     1381 ccccccgacg cggccggtca gacccgccgc cacatccgtc ggtgctcgat gccggcgtca
     1441 tcgaagatct cgccgtacgg cgtgtagccc aaccgctcgt agaagggcac cgcgtggacc
     1501 tgcgcgccga ggtagaccac ccgcagcccg cgcgccaccg cctgggcctc cagcgcccgc
     1561 accagggcgg cgccgatccc gtgcccgcgc gctcccgccc gcaccgcgag ccggccgatg
     1621 tgggccaccg gcccacaccc ggggtcgacc ccggcgaacc cgggtggctc gaccagcagc
     1681 cggccggtgc ccgtcgcgac cccgtcgacg cgggccagca cgtggtcggc ctccgggtcg
     1741 cgctcgtcgc gctcgagcgc cgcgggcacc gactgctcga cgacgaagac ctcccagcgg
     1801 atgtccaggg cctcgaccca ccgggagtcc ccctcgaccg cgctcaccac gtcgacggtc
     1861 agtgacggcg agctcatgcg gcgtcccgca gccggcgcag cgcctcggcc aggtcgtccg
     1921 ggtaggggct gtccacctgc agccagcggc cggtggaggg gtgctcaaga ccgaggcgta
     1981 cggcgtgcag ccactgccgg ccgagtccga ggcgggcggc cagcttgggg tcggcgccgt
     2041 aggtgaggtc gcccacgcac ggatggtgca gggccgacat gtgcacccgg atctggtggg
     2101 tgcgcccggt ctgcaggtgg acctcgagca ggctggcggc gcggaacgcc tcgagcgtgt
     2161 cgtagtgggt gatgctgtcc ttgccaccgg ccaccacggc ccacctgtag tcgtgggtgg
     2221 ggtgacggcc caccggggcg tcgacggtgc cgcgcagcgg gtccaggtgg ccctggacga
     2281 ccgcgtggta ggtcttctcg acggtgcgcg ccttgaacgc ccgtttgagc acggtgtagg
     2341 cccgctcgct cttggccacc accatgacgc cgctcgtgcc gacgtcgagg cggtgcacga
     2401 caccctgccg ctcgggcgcc ccggaggtcg agatgcggta gccggccgcc gcgagcccgc
     2461 cgaccaccgt cgggccggtc caccccggac tagggtgcgc cgccactccg atcggcttgt
     2521 cgacgatcac cacgtcgtcg tcgtcgtaga gcacgtgcag gccggggacc ggctcgggag
     2581 gggcggtggg cgccgcggcc gggctgggca cctcgacctc gagcactgcc ccggcgtgca
     2641 cccggtcgga cttgccgacc accacctggt cgaggaccac cgcgccggct gcggccagcg
     2701 cggcggcctg ggtgcgggag aacccgaaga gccgggccag cgcggcgtcg acccgctcgc
     2761 cgtcgagccc ctcgggcacg ggcaggatgc gcgcctcagc cacggctggt cgtgccgtcc
     2821 accgccacgc cgcgcagcgt gtagacgacc atggtgatcc ccgcaagcac gatcgcggag
     2881 tcggcgacgt tgaacaccgg ccagtagggc agctcgagga agtcgacgac gtggccccgc
     2941 agcggcgccg ggccgcggaa gagccgatcg ccgaggttgc cggcggcgcc gcccagcagc
     3001 aggcccagcg agagcgccca gggcaggctg acgaggcggc gagcgatgcg caggatgacg
     3061 atgacgacgg ccgccgcgac cacggtgaac tcgatggtgg ccccgccggc gagaccgaag
     3121 gcggccccgg agttgcgcac gatgcgcagc gtgagcagcc cgcccagcac ctccaccggc
     3181 tcgtggtcgg acagcaggtg tacggcgatc agcttggtca gctggtcaag cgccagcacc
     3241 accacggcca ccccgacgag cacgccgagc ttgccccggg cacgccgccg ggaccgccga
     3301 tgcgggggcg gcgcagggtc gggctcgctc agcggcgctc ctcgcgttgc ttgcaggtca
     3361 ggcacagcgt cgcccgcggg aacgcctgca gtcgcctctt gccgatcggg ttgccgcacg
     3421 actcacagac tccgtaggtg ccagcttcga tgcgtgccaa ggcacgcaat gtctgctcga
     3481 gcatgtcacg ggagttggcg gccagcgaca tctcgtgctc gcgctcgaag gtcttggtcc
     3541 ccgcatcggc ctggtcgtcg ccggctccct cgccggcatc gcgcagcagg tcggagatca
     3601 ccgactcggc gtcggtgatc tccgaacgca gccgggcgac ctccgactcg aggtcgccgc
     3661 gcacctgggc aagctcgtca ggggtccatg gcgactcgtc ctcgcgcacc gcgagccgcg
     3721 cggcggtggt ggcgaccgcc gacgccgaac gcgcccgctc ggagcggctg gccggcgact
     3781 tctgcgccgg gatcttcttg gtcgagccgg tcgcgggctt ggccttggcg cgcgtggcgc
     3841 cagagcggga ggccgtggcc ttggcggcag gcttggccgc cgacgtggtc gcagccggtt
     3901 tcgccgcggt ggcggccttc gccggcgcct tcgacctcgc ggtcgtcgcg gtccgcgccg
     3961 ccgaggcgga gcgggtcgtc ttggacgccg ccttcgtcgc cttggcgggc ttggcgcccg
     4021 gagcggcctt ggcggtcgat gctgccttgg tgcgcggcgt ggccttcttg gcgcttgggg
     4081 cggccgcgtt cttctgcgct ggctcggtcg cccgggccgc cctcgccggc cctgtcccac
     4141 gtgcggccat gaccgtcccc tctgtcacgc cgcgacggcg tgatcggcac ctgccctcgg
     4201 tcccggcagg ataggtcccc caggacgtgc cgacaacgca tcggcgcgaa ccggcgtgtc
     4261 agtgctcgcc gccgccggag gcctcctcgc ccgccgcacc cttcagctga cggcaccgtg
     4321 cggtcacgac ctccgcagcg gcggcgatcg ccacgagctt gtgccggctg gtcgcgccgc
     4381 gcagcagtga cacgtcgcgt cggcgtacgc cgaaggcctc ggcgactgcc cgcagcgccg
     4441 cctcggtcgc cgcaccgtcg accgctcggg cgctcacccg caccacgagc gcccccgctc
     4501 gctctccgcc gacgctggtg cgcgaggcgc c
//
LOCUS       DATIBB010000048         6246 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_678749, whole genome shotgun sequence.
ACCESSION   DATIBB010000048 DATIBB010000000
VERSION     DATIBB010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 6246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6246
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_678749"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(67..888)
                     /locus_tag="VLC50_01670"
     CDS             complement(67..888)
                     /locus_tag="VLC50_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014676020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HSF26203.1"
                     /translation="MPVSEPSSVYTHGHHESVLRSHTWRTAANSAAYLLPQIEPQMRV
                     LDIGCGPGTITADLAELVPQGAVTAIDSAPAIIERARATAAARGLVNVDFAVGDAHAL
                     EFPDGSFDVVHAHQVLQHVADPIAVLAEMGRVCAPSGLVAVRDADYAAMTWYPALPGL
                     AAWRELYEKVARANGGEPDAGRRLLSWARAAGFTDITASASVWCFATPEDRAWWGDLW
                     AERTVASGYARMAVDGGHATAAELETIAAAWREWSASDDGWFAVLHGEILARTSR"
     gene            976..1743
                     /gene="heR"
                     /locus_tag="VLC50_01675"
     CDS             976..1743
                     /gene="heR"
                     /locus_tag="VLC50_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010542476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heliorhodopsin HeR"
                     /protein_id="HSF26204.1"
                     /translation="MSVTAEQTHRRYRRLRAFNVVVGLVLAAQGLFMLLASNDLALPI
                     SASFLQADPVATTGPTPPELVFSLNIGATVAVFLLLAALDHLLVAAPGVHRWYEGGLT
                     RRANYARWIEYSVSASIMIVLIGLFVGIRDLAAVAGVFAATTAMILFGLLMERQQRPG
                     AADWSAFWFGALIGLVPWVLVVVYLVQPPTVPGFVYAIVVVQFVLFFSFAVNMALQYA
                     QVGRWRDYVHGEVAYIVLSLTAKSLLAWLIYANVLRS"
     gene            1870..2757
                     /locus_tag="VLC50_01680"
     CDS             1870..2757
                     /locus_tag="VLC50_01680"
                     /EC_number="3.1.1.99"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HSF26205.1"
                     /translation="MGDVRVEVALHVRASHAEGPMWDAATARLWWVDIVGQRVHCFDP
                     ASGRDTSWGVSGQPGGVILAADGEPVVATPDGLAMLDRGTGATSELVPIEADKPENRT
                     NDLKADRHGRVWVGTMAFDKRPRHAGLYRVDDAGVTTVVDELTISNGPAIDEERGLLY
                     LADTALSVVDVFDLDPATGSLTGRRRLLDLHEDGVWPDGMTVDDDGMLWVALGRAGAV
                     HRFRPDGRLDGVVTLPTSNPTSVAFGGADGRDLYVTTSWFDLDEEARREQPLAGAIFR
                     CRPGVAGPPAPRGQLPRHR"
     gene            complement(2786..3586)
                     /locus_tag="VLC50_01685"
     CDS             complement(2786..3586)
                     /locus_tag="VLC50_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015776439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HSF26206.1"
                     /translation="MASRRRVPLRGTCAQPHPCPRPLRLKVLAHVPYFEGLATDELDR
                     IDRRMVSLSWAEGDPLYFAGDPARHLYVLAEGQVKITHHTPSGGEVITDLLVPGELFG
                     TLSTLGQPSYPENAVALTTTCALRIDAAAFRTVLTEHPQVALRVLDDVAARFESARTE
                     IGRTASGSVPVRVAATLLRLADKLGHDRARGGTLLQLPLSRSDLAAMTASTPESVSRV
                     MSRLRQDGIIDSGRRWTTILDRERLEQIADGNPRPPHRAPDPPHTTPH"
     gene            3707..3949
                     /locus_tag="VLC50_01690"
     CDS             3707..3949
                     /locus_tag="VLC50_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019157287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-associated domain-containing
                     protein"
                     /protein_id="HSF26207.1"
                     /translation="MNATTPRTTTHTLLRAEGFSCPSCVTKIEKRVGRIDGVASVKVH
                     FASSRIEVDHDPAKASVDDVVAAVAKAGYQATPAAF"
     gene            3991..5925
                     /locus_tag="VLC50_01695"
     CDS             3991..5925
                     /locus_tag="VLC50_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010550399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HSF26208.1"
                     /translation="MNELARRLRGRWMVPTASGVLIVAGLLAGRAAGTAAAGDALLIG
                     GAAVAGAGIIRTAARALLLRVIGIDLLVSVATIGAVIIGEYWEAAAVTFLFAVGHALE
                     AATLNRTRSALAELVAVAPDVAIVLRDGRQAEVPATAVSAGETVLVKNGAKVPVDGDV
                     IDGSGAIDESSITGESMPVEKVAGDRVYAGTVAHGGLLQVRATGVGADTTLARIIHRV
                     EQAQDAKAKTQAFMDRFSAWYTPAIILLAIVVGMLTGDVVLALTLLVIGCPGALVISI
                     PVAIVAGIGRGARDGVLIKGGEFLETSSRITAVALDKTGTLTEGRPHLTDVVVLDPTL
                     DIADVLTWAARAEAGSEHPLARPILEAAGREGIEVRGVPESTKPVPGKGIVATVAGSR
                     VAVGTLALLADEGVRASDGAAAEVERLALAGRTPMVVAVDGRVAGVVAVADTTRPDAA
                     AMVAGLHAAGVTRVVMLTGDVPQVAAAVATDVGVDEVHASLLPEDKLEVVRRLQGEGH
                     VVAMVGDGVNDAPALATADIGVAMGAAGTGVAIETADIALMGDDLHKLPYALGLARRT
                     VATMRQNIAIALATVALLLLGVLVGGVTMAIGMLVHEASVLVVIANAMRLLRRTHRDV
                     VVAPTIASATDPADAAMMAR"
     gene            5993..>6246
                     /locus_tag="VLC50_01700"
     CDS             5993..>6246
                     /locus_tag="VLC50_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008256625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HSF26209.1"
                     /translation="MLAGELALAGVDVAIVERRTSQDLRGSRAGGLHARTIEAFDQRG
                     IADRFLAEGQVAQVAGFARVRFDISDFPTRHPYGLGLWQN"
BASE COUNT          900 a         2134 c         2263 g          949 t
ORIGIN      
        1 cctatttgca cagcgtggcg agaagcgact gcacccgctc gaccggcaca acatgccgct
       61 cccgcgtcag cgggaggtgc gagccaggat ctcaccgtgg aggacggcga accagccgtc
      121 gtcgctggcg ctccattccc gccaggcggc cgcgatggtc tcgagctctg cggccgtcgc
      181 gtgcccgccg tcgaccgcca tccgggcgta gccggacgcg acggtgcgct cggcccacag
      241 atcgccccac cacgcgcgat cctccggcgt ggcgaaacac cagaccgacg ccgatgcagt
      301 gatgtcggtg aagccggccg cccgggccca ggacagcagg cggcgtccgg cgtcgggctc
      361 gccaccgttg gcgcgggcca ccttctcgta gagctcgcgc cacgcggcca accccggcaa
      421 cgccgggtac cacgtcatcg cggcatagtc ggcatcgcgt acggcgacca gcccgctcgg
      481 cgcacagacc cggcccatct ctgcaagcac ggcgatcggg tcggcgacgt gctgaagcac
      541 ctggtgggca tggaccacgt cgaacgagcc gtcggggaac tcaagcgcgt gcgcgtcgcc
      601 gaccgcgaag tcgacgttga cgaggccacg tgcagcggcg gtggcccggg cccgctcaat
      661 gatcgccggc gccgagtcga tcgccgtgac cgctccctgc ggcaccagct cggccaggtc
      721 ggcggtgatc gtgccgggac cacagccgat gtcgagaacg cgcatctgcg gctcgatctg
      781 gggcaacagg taggccgcgg agttggccgc cgtacgccag gtgtgcgagc gaagcaccga
      841 ttcgtggtgt ccgtgcgtgt agacagagga cggctcagac accggcagcc ctcccgactc
      901 aaccgacagg caatgaccgg cacgctaacg tcaacgtcat cgggctttcg cggcgaccgg
      961 ggcgggggtg ccggcatgag cgtaacggcc gagcagactc atcggcgcta tcgcaggctg
     1021 cgtgccttca acgtcgtcgt gggcctcgtg ctggccgccc aaggtctctt catgctgctg
     1081 gccagcaatg acctcgcgct tccgatcagc gcctcgttcc tgcaggccga cccggtcgcc
     1141 acgactggac cgacaccgcc cgagctggtc ttctcgctga acatcggcgc caccgtcgct
     1201 gtgttcttgc tgctcgccgc gctcgaccat ctgctggtcg cggcgccggg ggtgcaccgc
     1261 tggtacgagg gtgggctgac ccgacgggcc aactacgcgc gctggatcga atactcggtg
     1321 agcgcgtcga tcatgatcgt gctgatcggc ttgttcgtcg gcattcgcga cctggccgcc
     1381 gtcgcaggcg tcttcgccgc caccaccgcg atgatcctct tcggtctgct catggagcgc
     1441 cagcaacgac ccggcgccgc cgactggagc gcgttctggt tcggcgcgct gatcgggctg
     1501 gtgccgtggg tgctggtggt cgtctatctc gtccagcctc cgaccgtgcc gggcttcgtc
     1561 tatgccatcg tcgtcgtcca gttcgtgttg ttcttcagct tcgccgtcaa catggcgctg
     1621 cagtacgcgc aggtgggtcg ttggcgtgac tacgtgcacg gtgaggtcgc ctacatcgtc
     1681 ttgagcctga ccgcgaagtc cctgctcgcg tggttgatct atgccaacgt gctgcgcagc
     1741 tgaggtcggc gcacctcggt gtgcgtacgg cgcctgcgcc gcggtgccgc cgtacggcat
     1801 ggtggtgacc gggacgcgtc gcaccgatgg gggcacggcg atgagacgag cgactggctg
     1861 aggcgcgcca tgggcgatgt tcgagtcgag gtggcgctgc atgtgcgggc ctcgcatgcc
     1921 gagggtccga tgtgggacgc cgcgaccgcc cggctgtggt gggtggacat cgtcggccaa
     1981 cgggtgcact gcttcgaccc ggcctccggc cgtgacacct cgtggggcgt gagcgggcag
     2041 ccgggcggcg tgatcctcgc cgccgacggc gagccggtcg tcgcgacccc tgacgggttg
     2101 gccatgctcg atcggggcac cggcgcgacc agcgagctcg ttccgatcga ggcggacaag
     2161 ccggagaacc gcaccaacga cctcaaggcg gatcgtcacg gccgggtctg ggtgggcacg
     2221 atggccttcg acaaacggcc acgccacgcc ggcctctacc gggtggatga cgccggcgtg
     2281 accaccgtgg tcgacgagct gaccatctcc aacgggccgg cgatcgacga ggaacgaggg
     2341 ttgctctacc tggccgacac cgcactgagc gtcgtcgacg tcttcgacct cgacccggcg
     2401 accggctcgc tcacgggccg gcgacgactg ctcgacctgc acgaggacgg ggtctggccc
     2461 gacggcatga ccgtcgacga cgacggaatg ctgtgggtcg ccctcggacg cgccggggcg
     2521 gtgcaccgat tccgtcccga tggccggctc gacggcgtgg tcacgctgcc cacgtcaaac
     2581 ccgacgtcag tggcctttgg cggggccgac gggcgcgacc tctacgtcac gacctcctgg
     2641 ttcgacctcg acgaggaggc ccggcgcgag cagccgctgg cgggcgcgat cttccggtgc
     2701 cgcccgggcg tcgcggggcc gccagcaccg cgcggtcagc tgccgcgcca ccgctagtcg
     2761 agatcatccg atgacttgcg catgatcagt gcggggttgt gtggggcggg tcgggtgccc
     2821 tgtggggcgg tcgcgggttt ccgtcggcga tctgctcgag acgctcgcgg tcgaggatgg
     2881 tggtccagcg tcggcccgag tcgatgatgc cgtcctggcg cagccggctc atcacgcgtg
     2941 agaccgactc cggcgtcgag gcggtcatcg cggccaggtc gctgcgggac aagggcagct
     3001 gcagcagcgt gccaccgcgg gcgcggtcgt ggccgagctt gtcggccagg cgcagcaacg
     3061 tggcagcgac ccgaaccggg accgacccgc tcgcggtgcg cccgatctcg gtgcgggccg
     3121 actcgaaacg cgcggccacg tcgtcgagga cgcgcagcgc cacctgcgga tgctcggtga
     3181 gcaccgtccg aaaggcggcc gcgtcgatgc gcagggcgca ggtcgtggtg agcgcgacgg
     3241 cgttctcggg atagctgggc tggcccaggg tgctcagcgt gccgaagagc tcgccgggga
     3301 ccagcagatc ggtgatgacc tcgccaccgc tgggcgtgtg gtgcgtgatc ttcacctgcc
     3361 cctcggccag cacgtagaga tggcgggccg ggtcgccagc gaagtagagc gggtcaccct
     3421 cggcccacga cagcgagacc atccgccgat cgattcggtc gagctcgtcc gtggcgagac
     3481 cttcgaaata aggcacgtgc gccaagacct tcaggcgcag cggacgtggg cacgggtgcg
     3541 gctgcgcgca cgtgccgcgc aacgggaccc gacggcggct ggccatggcc cgactctacg
     3601 agtttgccgt tgattgaccg ccgtcatggt ggggcgacct ccctcttcct agcgtcggag
     3661 cgagcaccca gaccgggtga gcccctacga aggaaggtcg ttgacggtga acgccaccac
     3721 cccccgcacc accacccaca ccctgctgcg cgccgaaggc ttctcctgcc cgtcgtgcgt
     3781 caccaagatc gagaaacggg tcggccggat cgacggcgtc gcgtcggtga aggtccactt
     3841 cgcctcctcg cgcatcgagg tcgaccacga cccggccaag gccagcgtgg acgacgtcgt
     3901 ggccgccgtt gccaaggccg gctaccaggc cacgcccgcc gccttctgac cagcgcgaga
     3961 gccaggcccc gaggcacagg agcgatgacg atgaacgagc tagcacgcag actgcgcggt
     4021 cgctggatgg tgccgaccgc gtccggcgta ctgatcgtgg ccggcttgct ggccgggaga
     4081 gctgcgggca cggccgcggc cggtgacgcc ctgctgatcg gcggcgccgc ggtcgccggt
     4141 gccggaatca tccgtacggc ggcgagggcg ctgctccttc gggtgatcgg gatcgacctg
     4201 ctggtctcgg tcgcgacgat cggcgcggtg atcatcggcg agtactggga ggccgccgcg
     4261 gtgaccttcc tgttcgcggt cgggcacgcg ctggaggcag cgacgctcaa ccggacccgc
     4321 tcggcgctgg cggagctggt cgccgtggcg cccgacgtcg ccatcgtgct acgcgacggg
     4381 cgccaagccg aggtcccggc gacggccgtg tccgctggcg agacagtgct ggtcaagaac
     4441 ggcgccaagg tgccagtcga cggcgatgtg atcgacggct ccggtgcgat cgacgaatcc
     4501 tcgatcaccg gcgagtcgat gccggtcgag aaggtcgccg gcgaccgcgt ctacgcgggc
     4561 accgtcgcgc acggcgggtt gctgcaggtg cgggccaccg gcgtcggtgc cgacaccacg
     4621 ctggcccgga tcatccaccg ggtcgagcag gcccaggacg ccaaggccaa gacgcaggca
     4681 ttcatggacc ggttctccgc ctggtacacg ccggcgatca tcctgctcgc catcgtcgtc
     4741 gggatgctca ccggagacgt cgtactcgcc ctgaccctgc tggtcatcgg ctgccccggc
     4801 gcgctagtga tctccattcc ggtggcgatc gtggcgggaa tcggtcgggg cgcgcgcgat
     4861 ggggtgctca tcaagggcgg cgagttcctc gagacgtctt cccggatcac ggcggtggcg
     4921 ctggacaaga ccggaacgct gaccgagggc cgcccgcacc tgactgacgt ggtcgtgctc
     4981 gacccgaccc tggacatcgc cgacgtgctg acgtgggcgg cgcgggccga ggccggctcg
     5041 gagcacccgt tggctcggcc catcctggag gcggccggac gcgagggcat cgaggtccgc
     5101 ggggtcccgg agtcgacgaa accggtgccg ggcaagggaa tcgtcgccac cgtggccgga
     5161 agccgggtcg ccgtcggcac cttggcactg ctggccgacg aaggcgtgcg ggccagtgac
     5221 ggggcagccg ccgaggtcga gcgcctggct ctggcggggc gcaccccgat ggtggtcgcc
     5281 gtcgatggcc gggtcgccgg cgtcgtcgcg gtggccgaca cgacccgccc cgacgccgcg
     5341 gcgatggtgg ccggcctgca cgccgccggg gtcactcgcg tggtcatgct caccggtgac
     5401 gtgccccagg tggcggccgc tgtcgcgacg gatgtcgggg ttgacgaggt gcacgcgtcg
     5461 ttgctacccg aggacaagct cgaggtcgta cggcgcctgc agggcgaagg ccatgtcgtg
     5521 gcaatggtcg gcgacggcgt caacgacgcg ccggcgcttg caaccgccga catcggtgtc
     5581 gcgatgggag cggccggcac cggcgtcgcg atcgagaccg ccgacatcgc actgatgggc
     5641 gacgacctgc acaagctgcc ttatgcgctt ggcctggccc gacgcaccgt cgccaccatg
     5701 cgtcagaaca tcgccatcgc gctggccacg gtggcactgc tgctgctcgg ggtgctcgtc
     5761 ggtggtgtga ccatggccat cggcatgctc gtgcatgagg catcagtgct cgtcgtcatc
     5821 gccaacgcga tgcggctgct gcgccgtacg catcgagatg ttgttgtcgc gccaaccatc
     5881 gcctcggcca cggacccggc cgacgcggcc atgatggcgc ggtgagcatc ggaggacctg
     5941 cactgtgagc gagcacgcgg tactgatcgt tggtgggggg cccaccgggc tgatgctggc
     6001 cggcgagctg gcgttggcgg gggtcgacgt tgcgatcgtc gagcggcgca ccagccagga
     6061 cctgcgcggc tcgcgcgccg gcggcctgca cgcacgaacc atcgaagcct tcgatcagcg
     6121 tggcatcgcc gatcgattcc tggcggaggg gcaggtggcc caggtcgccg gtttcgcccg
     6181 agttcggttc gacatcagcg acttcccaac ccggcatcct tacgggctcg ggctgtggca
     6241 gaacca
//
LOCUS       DATIBB010000049         8424 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_1205163, whole genome shotgun sequence.
ACCESSION   DATIBB010000049 DATIBB010000000
VERSION     DATIBB010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 8424)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8424)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8424
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1205163"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1311
                     /locus_tag="VLC50_01705"
     CDS             <1..1311
                     /locus_tag="VLC50_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020644377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HSF26210.1"
                     /translation="AYRGAGRPEATFAVERIMDELATELGMDPIELRRKNWIAHEEFP
                     FTTVAGLTYDSGNYEAATDKAVGLFKYDELRREQAERRERKDAVQLGIGVSTFTEMCG
                     LAPSRVLGSLDYGAGGWETSSIRMLPTGKVEVITGISPHGQGHETGFSQIVAERLGVA
                     FEDVQVIHGDTLTSHKGLDTYGSRSLVVGGIAVVKAADKVIEKAKVMAAHMLECDPSD
                     LEFAKGSFSVKGAPGNSKTIQEVALATFAAHDLPDGVEPSIDADATYDPENFSYPHGT
                     HLCAVEVDTITGRVTVRSYVCVDDVGTPINPLLIEGQVHGGVAQGISQALFEDAVFDA
                     EGNLVSGTFVDYLLPSAADLPNYVTDRTVTPATSNPLGVKGVGEAGTIAATPAVMNAI
                     LDALRPLGVTDINMPATPERVWRAVQQARAGAGVSAAATTGGAA"
     gene            1308..2159
                     /locus_tag="VLC50_01710"
     CDS             1308..2159
                     /locus_tag="VLC50_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012950877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HSF26211.1"
                     /translation="MIPASFDYVRPTSVDEAVRALGEAGEDAKVLAGGQSLIPVMRLR
                     LAYPSVLVDLGKVEELRGVRDDGDAIVIGAMTTHDTVLHDPLVTAHVPLVAEATATVA
                     DRQVRHRGTFGGALAHADPAGDLPAVVRALDASFVVAGPQGRRTISAADFFVDYLTSS
                     LAPGEVLVEVRIPKLGEGWGVAYEKFVRVAQAWAIVGVAAAVRRSNGTIAEARIGLTN
                     MGTTPVRASATEAALAGADAAGIEAAAASAADGAEPPSDLGGAADYRAHLATVLTARA
                     VHRAAGF"
     gene            2279..2959
                     /locus_tag="VLC50_01715"
     CDS             2279..2959
                     /locus_tag="VLC50_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009153411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HSF26212.1"
                     /translation="MELEHSFTVPAGIDQAWATLLDVEKVAPCMPGATLESVNGDEFT
                     GKVKVKVGPITITYGGQAAFRDRDDAAHTVVIDASGKESRGSGTAKATVHAQMQAEGE
                     SSTKVVVRTDLNVTGRPAQFGRGVMNDVSAKLIGQFADCLAAKMAAPAEPEAPAEAPA
                     AAGTDGAGTGAPAAPAAPRPVAAPRPTPEAIDLFETAGAPVLKRLAPVIGGLFVLLVL
                     WRLVRRKG"
     gene            complement(3062..3772)
                     /locus_tag="VLC50_01720"
     CDS             complement(3062..3772)
                     /locus_tag="VLC50_01720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF26213.1"
                     /translation="MSPAAPTLVLLHSPLAGPASWGGLPEALRAAGREVITVDVCEDD
                     EPPYAPRYVASVARQVNAAGPTGVVLVAHSGAGALVPALAIAQRAAHRPVRGYLFVDA
                     VLPRWMPGNRLDLLRAEDAETAAELREHLEAGGRFPTWTDAELAETITDSEWRQAVLK
                     DLHPRGLDFFTEALPLPVDWPDAPCGFVRTSAAYDVHARAAAARGWAVRLREGGHFAG
                     CDDPGGLAAAVLDVAGPW"
     gene            3813..4427
                     /gene="pyrE"
                     /locus_tag="VLC50_01725"
     CDS             3813..4427
                     /gene="pyrE"
                     /locus_tag="VLC50_01725"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006592091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HSF26214.1"
                     /translation="MSSRTTPAAPSPSAAPESAPPSQAQVDRAALLALVRDQAIVHGR
                     VTLASGKEADYYVDLRRVTLDGVAAPLVGRVMLDLVADLDFDAVGGLTMGADPVAAAM
                     LHAAAARGRRLDAFVVRKSEKSHGLQRRIEGPDVAGRRVLAVEDTSTTGGSVLTAVEA
                     LREAGAHVVAVAVIADRDTGARERVAAAGLDYRAAYSLADLGLA"
     gene            4432..5184
                     /locus_tag="VLC50_01730"
     CDS             4432..5184
                     /locus_tag="VLC50_01730"
                     /inference="COORDINATES: protein motif:HMM:NF022779.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3137 domain-containing protein"
                     /protein_id="HSF26215.1"
                     /translation="MLGDATPFLLLLVGIGLVVVVAVGAWTLDRKRREAMFAWCATHG
                     WSYVGEDESLTYRWSGAPFGTGERRRARNVMRGPVGDRQAVVFDYSYETSSTDSKGNR
                     TTTTHRFAVVAVGLPVPLPRLQVTPDNVFKRAAAAIGLGDEDIHLESDEFNKRFRVTA
                     RDRKFAFDVLHPRLMHALLAAEPVAWRFEGSDLLSWDSGRLGPDQALVRAMMLSSVVD
                     AVPTFVWKDRGYDPSAAGPSPANPWQGSEETS"
     gene            5178..5741
                     /locus_tag="VLC50_01735"
     CDS             5178..5741
                     /locus_tag="VLC50_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005078718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HSF26216.1"
                     /translation="MVNTVGWIILAVAVLTGLWLVLSYNRLVSLRNLVAESWRQIDVE
                     LHRRHDLIPNLVETVKGYAAHERAVLDEVTARRATAVTPGSTPAAQAQQENLLGQALG
                     RLLAIAEAYPDLKASANFLELQRQLAETEDRIAAGRRFYNANVRALNTACESFPTNAV
                     AGWFGFHQAEYFEVDDPTVRAVPSVDF"
     gene            complement(5746..6129)
                     /locus_tag="VLC50_01740"
     CDS             complement(5746..6129)
                     /locus_tag="VLC50_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013116548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HSF26217.1"
                     /translation="MIVDIDPASPVPPYEQIRTQVVALVESGGLTVGARLPTVRQLAI
                     ELGVAPGTVAKAYRELETAGVVITKGRHGTLVAEPAVQLEATERRRRLDAAAEAFVRE
                     AGALGVSLAEAQDAVRRLGLTRLRD"
     gene            complement(6300..6761)
                     /locus_tag="VLC50_01745"
     CDS             complement(6300..6761)
                     /locus_tag="VLC50_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017948431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HSF26218.1"
                     /translation="MAADSSYALDAVDRSVLDVLAGNARASIAEIARTVGLAPSTVAE
                     RVRRLEHRGVIRGYRLDVDLSKLGFALTALVRLTGQNVNNHALLGKVVDRVPEVLQCH
                     RVTGQEGWVLTVVARDVAHLEQVIETLSGYGLPATSIVLSSWTATHLPPTD"
     gene            6929..8299
                     /locus_tag="VLC50_01750"
     CDS             6929..8299
                     /locus_tag="VLC50_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HSF26219.1"
                     /translation="MRTIIEPFRIKSVEPIEMSTPEQRDQWLTEVGYNLFRIPADRVL
                     IDLLTDSGTSAMSAAQWGAVMAGDESYAGARSWFRFEEAVKRVFGFEHVVPTHQGRAA
                     ERILFGCLVKPGDVVPSNAHFDTTRANIEYLGGRGLDLPCAEAADPTTDEPFKGNMDV
                     EALEKLLAGEADAVRLVMLTITNNTGGGQPVSMANIRAVSEVAHRYGKPLYIDACRFA
                     ENAWFIKTREAGYADVAVPEIVREMFSYADGATMSAKKDALVNIGGFLCTRDPSLAAK
                     EKDLLILTEGFPTYGGMAGRDLEAIAVGLDEVLHEDYLRYRIVSTAYVVDHLHAAGVP
                     VLRPAGGHAVYLDAGRFLPHVPALQYPGQALAVELYRHGGIRGCEIGTVMAGRDPATG
                     EERPARIELVRLAIPRRVYTQSHMDYVVEAVTEVVERRTSIPGYRIVEEPSFLRHFTA
                     TFAPIA"
BASE COUNT         1180 a         3118 c         3026 g         1100 t
ORIGIN      
        1 gcctaccgcg gcgccggccg gcccgaggcc acgttcgccg tcgagcggat catggacgag
       61 ctggccaccg agctgggcat ggacccgatc gagctgcgcc gcaagaactg gatcgcccac
      121 gaggagttcc cgttcaccac cgtggccggg ctgacctacg actcgggcaa ctacgaggcc
      181 gccaccgaca aggcggtcgg gctgttcaag tacgacgagc tgcgccgcga gcaggccgag
      241 cgccgagagc ggaaggacgc cgtacagctg ggcatcgggg tctcgacgtt caccgagatg
      301 tgcgggctgg ccccgtcgcg ggtgctgggc tcgctcgact acggcgccgg cggctgggag
      361 acctcctcga tccggatgct gcccaccggc aaggtcgagg tgatcaccgg tatctccccg
      421 cacgggcagg gccacgagac cgggttcagc cagatcgtgg ccgagcggct cggggtggcc
      481 ttcgaggacg tgcaggtcat ccacggcgac acgctgacca gccacaaggg cctggacacc
      541 tatggctccc gctcgctggt ggtcggtggc atcgcggtgg tcaaggccgc cgacaaggtc
      601 atcgagaagg ccaaggtcat ggccgcgcac atgctcgagt gcgacccgag cgacctggag
      661 ttcgccaagg gctcgttcag cgtcaagggc gcgccgggca acagcaagac catccaggag
      721 gtcgcgctgg ccaccttcgc cgcgcacgac ctgcccgacg gggtcgagcc gtcgatcgac
      781 gccgacgcga cctatgaccc ggagaacttc tcctacccgc acggcaccca cctgtgcgcg
      841 gtcgaggtgg acaccatcac cggccgggtc acggtgcgct cctacgtctg cgtcgacgac
      901 gtcggcaccc cgatcaaccc gctgctcatc gaggggcagg tgcacggcgg ggtggcccag
      961 ggcatctcgc aggcgctgtt cgaggacgcc gtcttcgacg ccgagggcaa cctggtcagc
     1021 ggcacgttcg tcgactacct gctgcccagc gcggccgacc tgcccaacta cgtgaccgac
     1081 cgcaccgtga ccccggcgac gagcaacccg ctcggcgtca agggcgtcgg cgaggctggc
     1141 accatcgcgg ccaccccggc cgtgatgaac gcgatcctcg acgcgctgcg cccgctgggg
     1201 gtcaccgaca tcaacatgcc ggccacaccc gaacgggtct ggcgggccgt ccaacaggcc
     1261 cgcgctggcg cgggcgtctc agccgctgcc accaccggag gtgccgcgtg atccccgcgt
     1321 cgttcgacta cgtgcgcccc accagcgtgg acgaggcggt gcgcgcgctc ggcgaggccg
     1381 gcgaggacgc caaggtgctc gccggcgggc agagcctcat ccccgtgatg cggctgcggc
     1441 tggcctaccc ctcggtgctc gtcgacctgg gcaaggtcga ggagctgcgc ggggtgcgcg
     1501 acgacggtga cgcgatcgtc atcggcgcca tgaccaccca cgacaccgtg ctgcacgacc
     1561 cgctggtcac cgcgcacgtg ccgctggtgg ccgaggccac cgccacggtg gccgaccggc
     1621 aggtgcgcca ccgcggcacc ttcggcggcg cgctggctca cgccgacccg gccggtgacc
     1681 tgccggcggt ggtgcgggcg ctggacgcgt cgttcgtggt ggccggcccg caggggcggc
     1741 ggaccatctc ggccgccgac ttcttcgtcg actacctcac cagctcgctg gcgcccggcg
     1801 aggtgctcgt cgaggtgcgc atccccaagc tcggcgaggg ctggggcgtg gcctacgaga
     1861 agttcgtccg ggtcgcgcag gcctgggcga tcgtcggggt cgcggccgcc gtacgccggt
     1921 ccaacgggac gatcgccgag gcgcgcatcg ggctgaccaa catgggcacc acgccggtgc
     1981 gggccagcgc gaccgaggcc gcactggccg gggccgacgc cgccggcatc gaggcggccg
     2041 ccgcctcggc cgccgacggg gccgagccgc cgagcgacct gggtggggcg gccgactacc
     2101 gcgcccacct ggcgacggtg ctcaccgcgc gcgccgtgca ccgggccgcc gggttctgat
     2161 cgacacctcc ccgcactggt cccgcacccg ctgtgacagg ctgtagcgga ctgcaggacc
     2221 agtgcggggc ctcagcccgc atcgcccgga tcgatcgacg acgtgaggat caggacagat
     2281 ggagctcgag cactcgttca ccgtcccagc cggcatcgac caggcctggg ccaccctcct
     2341 cgacgtcgag aaggtcgccc cgtgcatgcc cggcgccacg ctggagagcg tcaacggtga
     2401 cgagttcacc ggcaaggtga aggtcaaggt cggcccgatc acgatcacgt acggcgggca
     2461 ggcggccttc cgcgaccgcg acgacgcggc gcacaccgtc gtcatcgacg cgtccggcaa
     2521 ggagtcgcgc ggctcgggca ccgccaaggc gaccgtgcac gcgcagatgc aggccgaggg
     2581 tgagagctcg accaaggtcg tcgtgcgcac cgacctcaac gtcaccggcc ggccggccca
     2641 gttcggccgc ggcgtgatga acgacgtgtc ggccaagctg atcggccagt tcgccgactg
     2701 cctggccgcc aagatggccg ccccggccga gcccgaggcg ccggccgagg cccccgcggc
     2761 agctggcacc gacggcgcgg gcaccggcgc tcccgctgct ccggccgcac cccgcccggt
     2821 cgcggccccc cgcccgacgc ccgaggcgat cgacctgttc gagaccgccg gcgcgccggt
     2881 gctcaagcgg ctcgccccgg tcatcggcgg gctgttcgtg ctgctcgtcc tgtggcggct
     2941 ggtccgccgc aagggctgac ccggcttact cgccgccgcc cccgttactc gctgaggcca
     3001 ccgtcacgga cccgtccgac ggtggcctca gcgtgtattg ggcgggtggg cgagtagacg
     3061 atcaccaggg gccggcgacg tcgagcacgg cggcggccag gccacccggg tcgtcgcagc
     3121 cggcgaagtg cccgccctcg cgcaggcgca ccgcccagcc gcgggccgcg gcggccctgg
     3181 cgtgcacgtc gtacgccgcc gaggtgcgca cgaacccgca cggcgcgtcg ggccagtcca
     3241 ccggcagcgg cagcgcctcg gtgaagaagt cgagcccgcg cgggtgcagg tccttgagca
     3301 ccgcctgacg ccactccgag tcggtgatcg tttcggccag ctcggcgtcg gtccaggtcg
     3361 ggaaccgccc gccggcctcg aggtgctcgc gcagctcggc ggccgtctcg gcgtcctcgg
     3421 cccgcagcag gtcgagccgg ttgcccggca tccatcgcgg cagcaccgcg tcgacgaaca
     3481 ggtagccgcg gaccggccgg tgcgccgccc gctgggcgat cgccagagcc ggcaccagcg
     3541 ccccggctcc ggagtgcgcg acgagtacga ccccggtcgg ccccgcggcg ttgacctggc
     3601 gggcgacgct ggcgacgtag cggggggcgt agggcggctc gtcgtcctcg cacacgtcga
     3661 cggtgatcac ctcgcgcccg gcagcgcgca acgcctcggg cagcccgccc cacgaggccg
     3721 gcccggccag cgggctgtgc agcaggacca acgtgggcgc ggcggggctc atgggtgaca
     3781 gcctgccgca ccgactgccc tagtctgcgg gcatgtcctc gcgtacgacg cccgccgctc
     3841 cctcgccgtc cgcggcgccc gagtcggccc cgccctcgca ggcccaggtc gaccgagccg
     3901 ccctgctcgc cctcgtgcgc gaccaggcga tcgtgcacgg ccgggtcacc ctggcctcgg
     3961 gcaaggaggc cgactactac gtcgaccttc gccgggtcac cctcgacggc gtggcggccc
     4021 cgctggtcgg tcgggtgatg ctcgacctgg tcgccgacct tgacttcgac gccgtcggcg
     4081 ggctgaccat gggcgccgac ccggtggcag cggcgatgct gcacgcggcc gccgcgcgcg
     4141 gacgccggct cgacgcgttc gtcgtacgca agtctgagaa gtcccacgga ctgcagcgcc
     4201 gcatcgaggg gcccgacgtg gccgggcgcc gggtgctcgc ggtggaggac acctcgacca
     4261 ccggcggctc ggtgctcacc gcggtcgagg cgctgcgcga ggccggcgcg cacgtcgtgg
     4321 ccgtcgcggt gatcgccgac cgcgacaccg gcgcgcgtga gcgggtggct gcggctgggc
     4381 tcgactaccg cgcggcctac tcgctggccg acctcgggct ggcctgagcc gatgcttggc
     4441 gacgcgacgc cgttcctgct gctgctggtc ggcatcgggc tggtcgtcgt ggtcgcggtc
     4501 ggcgcctgga cgctcgaccg caagcggcgc gaggcgatgt tcgcctggtg cgcgacgcac
     4561 gggtggagct acgtcggcga ggacgagtcg ctgacctatc gctggtcggg cgccccgttt
     4621 ggcaccgggg agcgccggcg ggcccgcaac gtcatgcgcg ggccggtcgg ggaccgccag
     4681 gcggtggtct tcgactactc ctacgagacc tcctcgaccg actccaaggg caaccgcacc
     4741 accaccacgc accggttcgc ggtggtcgcc gtcgggctgc cggtgccgct gccgcggctg
     4801 caggtcacgc cggacaacgt gttcaagcgg gccgccgcgg cgatcgggct gggcgacgag
     4861 gacatccacc tggagagcga cgagttcaac aagcggttcc gggtcaccgc gcgcgaccgc
     4921 aagttcgcgt tcgacgtact gcatccccgg ctgatgcacg cgctgctcgc cgccgagccg
     4981 gtcgcgtggc ggttcgaggg cagcgacctg ctcagttggg acagtggcag gctcggcccc
     5041 gaccaggcgt tggtgcgggc gatgatgctc tcaagcgtgg tcgacgcggt gccgaccttc
     5101 gtctggaagg accgcggcta cgacccatcg gcggccgggc catctccggc caacccctgg
     5161 caaggcagcg aggagacatc gtgaacaccg tcgggtggat catcctggcc gtcgcggtcc
     5221 tgaccgggct ctggctcgtg ctcagctaca accggctggt ttcgctgcgc aacctggtcg
     5281 ccgagtcgtg gcgccagatc gacgtcgagc tgcaccgacg ccacgacctg atccccaacc
     5341 tggtcgagac ggtcaagggc tacgccgccc acgagcgcgc ggtgctcgac gaggtgaccg
     5401 cccggcgagc caccgcggtg accccggggt ccacgccggc ggcccaggcg cagcaggaga
     5461 acctgctcgg gcaggcgctc ggtcggctgc tcgccatcgc cgaggcctac ccagacctga
     5521 aggccagcgc caacttcctc gagctgcagc gccagctggc cgagaccgag gaccgcatcg
     5581 ccgcgggccg gcgcttctat aacgccaacg tgcgcgcgct caacaccgcg tgcgagtcgt
     5641 tcccgaccaa tgcggtggcc ggctggttcg gcttccacca ggccgagtac ttcgaggtcg
     5701 acgaccccac cgtgcgcgcc gtccccagcg tcgacttctg accggctagt cgcgaaggcg
     5761 ggtcagaccc aggcgccgta cggcgtcttg ggcctcggcc agcgatacgc cgagcgcgcc
     5821 tgcctcccgg acgaacgcct cggccgcggc gtcgagccgc cggcgtcgct cggtcgcctc
     5881 gagctggact gccggctcgg cgaccagtgt gccgtgccga cccttggtga tcaccacgcc
     5941 ggccgtctcg agctcgcggt aggccttcgc cacggtcccc ggcgcaaccc cgagctcgat
     6001 ggccagctgg cgcaccgtcg gcagccgcgc cccaaccgtg agcccgccgg actcaaccag
     6061 cgcgacgacc tgggtgcgga tctgctcgta cggcgggacc gggctggccg ggtcgatgtc
     6121 cacgatcacg cgctcaccgt ctcacgtgcc tggcggccca gagccggtgg cggacgctag
     6181 ggacggacca accgcgccgg gacgcactgg ctgagcacgc gacgtcgtgg gctgtgggcg
     6241 ttactccgtc cctagcgttc ggaccgtgcg ggcagagggc ggcgggggag gggcggcggt
     6301 cagtcggtgg ggggcaagtg ggtggccgtc cacgacgaca ggacgatcga cgtggccggc
     6361 agcccgtagc cggacagcgt ctcgatcacc tgctcgaggt gggccacgtc gcgcgcgacc
     6421 acggtgagca cccagccctc ctggccggtg acccggtggc actgcagcac ctcgggcacc
     6481 cggtcgacca ccttgccgag cagcgcgtgg ttgttgacgt tctgcccggt caggcgcacc
     6541 agcgcggtca gcgcgaaccc cagcttgctc aggtcgacgt cgagtcggta gccgcggatc
     6601 accccgcgat gctcgagccg gcgcacccgc tcggccaccg tgctcggcgc cagcccgacg
     6661 gtgcgggcga tctcggcgat gctcgcgcgg gcgttgccgg cgagcacgtc gagcacgctc
     6721 cgatcgacgg catcgagggc gtacgacgaa tcagcggcca cgccccgagt atgccggcag
     6781 atcaacggca gaacgccgcc aggtgccccc ggaacggcga gatcaccacc ggaagtccgg
     6841 catagcgttg agacgccgag gtgccggccg ccgccccccg ttggcggccg gcacccggcc
     6901 ttcgccgtac gccgtcaagg agcaccccat gcgcacgatc atcgagccgt tccggatcaa
     6961 gagcgtcgag ccgatcgaga tgtccacccc cgagcagcgc gaccagtggc tgaccgaggt
     7021 gggctacaac ctgttccgga tcccggccga ccgggtgctg atcgacctgc tcaccgactc
     7081 gggcacctcg gcgatgagcg cggcgcagtg gggcgcggtg atggccggcg acgagtccta
     7141 cgccggcgcg cggtcctggt tccggttcga ggaggccgtc aagcgggtct tcgggttcga
     7201 gcacgtcgtg cccacccacc agggccgcgc cgccgagcgg atcctcttcg gctgcctggt
     7261 caagcccggc gacgtcgtac cgtccaacgc tcacttcgac accacccgcg ccaacatcga
     7321 gtacctcggc gggcgcgggc tcgacctgcc ctgcgccgag gcggccgacc cgaccactga
     7381 cgagccgttc aagggcaaca tggacgtcga ggcgctggag aagctgctcg ccggtgaggc
     7441 cgacgccgta cgcctggtca tgctgacgat caccaacaac accggcggcg ggcagccggt
     7501 gtcgatggcc aacatccgcg cggtctccga ggtggcccac cgctacggca agccgctcta
     7561 catcgacgcc tgccgcttcg ccgagaacgc ctggttcatc aagacccgcg aggccggcta
     7621 cgccgacgtc gcggtgcccg agatcgtgcg cgagatgttc tcctacgccg acggcgcgac
     7681 catgtcggcc aagaaggacg cgctggtcaa catcggcggc ttcctgtgca cccgcgaccc
     7741 gtcgctggcc gccaaggaga aggacctgct catcctcacc gaaggcttcc cgacgtacgg
     7801 cgggatggcc gggcgcgacc tcgaggcgat cgcggtcggg ctcgacgagg tgctgcacga
     7861 ggactacctg cgctaccgga tcgtctccac ggcctacgtc gtcgaccacc tgcacgccgc
     7921 cggggtgccg gtgctgcgcc cggccggcgg gcacgcggtc tacctcgacg ccggtcggtt
     7981 cctgccgcac gtgccggcgc tgcagtaccc gggccaggcg ctcgcggtcg agctctaccg
     8041 gcacggcggc atccgcggct gcgagatcgg caccgtgatg gccgggcgcg acccggcgac
     8101 cggcgaggag cgcccggccc ggatcgagct ggtccggctg gcgatcccgc ggcgggtcta
     8161 cacccagtcg cacatggact acgtcgtcga ggcggtcacc gaggtggtcg agcgccgtac
     8221 gtcgatcccg ggttaccgca tcgtcgagga gccgtcgttc ctgcgccact tcaccgcgac
     8281 gttcgcgccg atcgcctgac cgcgccgcgc cgggcggacc ggggcggttg gtcccgaccc
     8341 tgcccggtgc cggccggcgc aggctggagg catgacaacc aggctcgacg tgcccggcgg
     8401 cgccaccgtg gtcggtcagc cggt
//
LOCUS       DATIBB010000050        13273 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_417442, whole genome shotgun sequence.
ACCESSION   DATIBB010000050 DATIBB010000000
VERSION     DATIBB010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 13273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13273
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_417442"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..278)
                     /locus_tag="VLC50_01755"
     CDS             complement(<1..278)
                     /locus_tag="VLC50_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005478206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HSF26220.1"
                     /translation="MPLTPEDVANKQFSSVRLREGYDMEEVDQFLDDVEQEIARLLRE
                     NDDLRGRLSASERAVAEAETTAAEATRKLNEVEGGAADATRKVREAERR"
     gene            complement(444..746)
                     /locus_tag="VLC50_01760"
     CDS             complement(444..746)
                     /locus_tag="VLC50_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015936786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HSF26221.1"
                     /translation="MRTLVGVIVTLLWLYFLVLIARLVLDYVQMFARQWRPQGVVLVL
                     CEVVYSLTDPPLKALRRVIPPLRLGRISLDLSFLVLILAIQILIPIVTRIGAGLPS"
     gene            complement(783..1310)
                     /gene="sepF"
                     /locus_tag="VLC50_01765"
     CDS             complement(783..1310)
                     /gene="sepF"
                     /locus_tag="VLC50_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017612963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HSF26222.1"
                     /translation="MAGAMRKMAVYLGLVEDEERYDDSYDGYDPSPARTEAASPSAHP
                     RQAPTVREEPRSTVTEMAARPDRRAGVETRVSARPRPADPYRITTIHPRTYNDARQIG
                     EAFRDSTPVIMNLTEMDDSDAKRLVDFAAGLIFGLRGSIERVTNKVFLLSPANVEVTA
                     EDKALMVESGFFNQS"
     gene            complement(1374..2081)
                     /locus_tag="VLC50_01770"
     CDS             complement(1374..2081)
                     /locus_tag="VLC50_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013131662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HSF26223.1"
                     /translation="MSAQESRRDELAANLAAVRRRIDAACARAGRDPAAITLVVVTKN
                     WPASDVRALAGLGVRDVGENRDQEAAAKHDACADLDQLVWHFVGQLQTNKARSVARYA
                     DVVHSVDRARLVAALDRAAEAEGRRLTCLVQVDLDGGVGRGGARPDTVLDLAETVASS
                     RQLALGGLMAVAPLGTDPAPAFARLGELAVTVREHHPQATIVSAGMSGDLEAAVAAGA
                     THLRIGTAVLGSRRAVR"
     gene            complement(2078..2812)
                     /gene="pgeF"
                     /locus_tag="VLC50_01775"
     CDS             complement(2078..2812)
                     /gene="pgeF"
                     /locus_tag="VLC50_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020552924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HSF26224.1"
                     /translation="MFAWRERLTDPADRPVVELAVTDRYGGVGQAPYDELNLADHVGD
                     REQVVRDNRARLAAAIGLDADRVVFMRQVHGRSVALVEQGWAGSAPEADAMVTRVPGL
                     ALAVLVADCTPVLLADPQAGVLGVAHAGRRGLAAGVVDALVEVMRAQGAREIIGRVGP
                     SICGRCYEVPLAMREEVAAVAPEARAMTAHGTPALDVGAGVMAQLAPHCDELAWLPGC
                     SAERADLFSHRRDGATGRYAGLAWLA"
     gene            complement(2815..4068)
                     /gene="ftsZ"
                     /locus_tag="VLC50_01780"
     CDS             complement(2815..4068)
                     /gene="ftsZ"
                     /locus_tag="VLC50_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HSF26225.1"
                     /translation="MAAPQNYLAVIKVVGIGGGGVNAVNRMIEVGLKGVEFIAINTDA
                     QALLMSDADVKLDVGRELTRGLGAGADPEVGRKAAEDHAEEIEEVLKGADMVFVTAGE
                     GGGTGTGGAPVIARIARSLGALTIGVVTRPFSFEGRRRAGSAETGIAALRAEVDTLIV
                     IPNDRLLSISDRAISVLDAFRSADQVLLQGVSGITDLITTPGLINLDFADVKSVMQGA
                     GSALMGIGSARGDARAVEAAQMAISSPLLETSIDGAHGVLLSIAGGSDLGLFEIDEAA
                     KLVHEAAHPEANIIFGAVIDDALGDEVRVTVIAAGFDDVAVRPAVRTAGDARVGDATA
                     GDAAARPEPARVSGPAGSVPSAAPAAAAATAVATEDPSRTTPASAGRAVPEPTTARPE
                     PTRPIPGRRIVFDDTDDLDVPDFLK"
     gene            complement(4301..4954)
                     /locus_tag="VLC50_01785"
     CDS             complement(4301..4954)
                     /locus_tag="VLC50_01785"
                     /inference="COORDINATES: protein motif:HMM:NF015739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HSF26226.1"
                     /translation="MALAVLAVLGLGAGGSWLVLGSPVLAVDRVVVEGADRLPTQAIL
                     DAAHVPRGAAMVRLDRAAIADRVRTLAPVADVEVDRRWPSTVALVVTERTPAAVLSVG
                     HGFRLVDADGVAFAAAAKRPKGLPLISGVPAAAVDRLRAATVVAAGLPDDLAKHVVSV
                     SARSADAVVLQLTHHREVVWGAASDASAKVAVLRVLMQTQQAAVYDVSAPAAPTTRR"
     gene            complement(5038..6459)
                     /gene="murC"
                     /locus_tag="VLC50_01790"
     CDS             complement(5038..6459)
                     /gene="murC"
                     /locus_tag="VLC50_01790"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013131659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HSF26227.1"
                     /translation="MSLVELPAQVPPADELGRVHFIGIGGAGMSAIARIMRARGMTVS
                     GSDARESAVLQALQAAGAITYVGHAAHQLAGVDTVVVSTAIRHDNPELAEARRLGLRV
                     LPRAAALAAVMTGRRAVTVAGTHGKTSTTSLLTVALRHCGLDPSFAIGGELGELGTNA
                     HDGTGDVFVAEADESDGSFLAYRPTGAVVTNIEADHLDTYGTPAAYQEAFDRFAALLP
                     PDGFLVASADDPGAARLAARTGERGVRTLTYGEAAGADLRVEAVETVGMSARFEVVAH
                     GQRLGAVALRVPGRHNVLNATAALGAGLALGLPFGALREGLARFTGTRRRLEPKGEAA
                     GVRVIDSYAHHPSELVADLRAARTIAGGGRVVAAFQPHLYSRTQIFARELGEALGLAD
                     EVVVMDVYGAREEPVPGVSGALVAAAVPLAKDHVLFEPTWSAVPDRLVERAEEGDLVL
                     TLGAGDVTRIGPEVLARLAARTA"
     gene            complement(6459..7550)
                     /gene="murG"
                     /locus_tag="VLC50_01795"
     CDS             complement(6459..7550)
                     /gene="murG"
                     /locus_tag="VLC50_01795"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018837708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HSF26228.1"
                     /translation="MRVLLAGGGSTGHVAPLLALADTLVRRDPDVAVLALGTAEGLES
                     RLVPERGYRLAFVPRVPLPRRPSADLLRLPARLVDAVRAAGQAIEEVGAEVVVGFGGY
                     VSTPAYLAARRRRLPIVVHEANSRPGLANRLGARLTPWVATTFPGTRLPHALRTGLPL
                     RREITLLDRPAARPGARAALGLAPTRPTVLVVGGSLGATRLNEVVPAVAADLCAAGVQ
                     VLHASGAGKEFPSPARPGGPAYVVTPYLDRMDLAYAAADLVIGRAGAGTVSELSALGL
                     PAVYVPLPVGNGEQRLNAAPVVAAGGGLLVEDAALTPDWLRSHVLPLVTDPDRLAAMG
                     AAAAAFGRPDADERLADMVERAVAGRGGR"
     gene            complement(7556..8767)
                     /gene="ftsW"
                     /locus_tag="VLC50_01800"
     CDS             complement(7556..8767)
                     /gene="ftsW"
                     /locus_tag="VLC50_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019877660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HSF26229.1"
                     /translation="MTTAAAPRLREAAREPTRALDRPLASYYLVLGSTALLVLLGLVM
                     VLSASSVESYVQTKSSFTIFQRQAMWVAVALPLMWAASRLPVRFYRRMAPIGLLAALA
                     LLALVPLVGVEVKGNRNWLDLAGMRMQPSELAKLALIVWGADLLARKHRLLSQWKHLL
                     VPLVPVSVLVLALVMLGNDLGTVLVIMAIVGALLFLVGAPLRLFAGSLLAFGTIVALL
                     VVSSPNRMKRLGSWRTPSQDYLDAGWQAVHGHYALATGGWWGLGLGASRQKWGALPEA
                     HTDFIFAIIGEELGLIGTLTVLLLFIALAYGGFRVAVRSNDLFVRLAAGGVTAWIMIQ
                     AVVNIGAVLGLLPIVGVSLPLVSYGGSSLITVLLGLGMLLSFARSTPEAAAVLRSRPA
                     PLRRLARQGRR"
     gene            complement(8764..10212)
                     /gene="murD"
                     /locus_tag="VLC50_01805"
     CDS             complement(8764..10212)
                     /gene="murD"
                     /locus_tag="VLC50_01805"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019067756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HSF26230.1"
                     /translation="MSGRLTGPGQELPWPSRHVVVTGLGVSGKAVAIALAGLGVADLQ
                     VVDAGTSERHQQAAAALREVGVRVSLGEAASRELPAGTDLVITSPGWRPDQPLLRAAA
                     GRGVPVWGDVELAWRLRPAGQQWLALTGTNGKTTTVRMLEAMLLADGRRALAAGNVGT
                     SLVATVLEPTPYDVLAVELSSFELHWAHTLAPLAGAVLNLAPDHIDWHGSMDAYGAAK
                     GKLLRWAQAAAVYNVEDAATEALLPRTGADGARAVGFTRGVPGAGMLGVEQGVLLDRA
                     FGPSASEPVELAAVADITPAAPHNVANALAAAALARAAGVGADAVRDGLRSFRPGAHR
                     IAHVATIDGVGFVDDSKATNPHAAAASLAAYDSVVWIAGGLAKGATFDDLVARAGTAL
                     RAVVLLGRDRDVIRAALARHAPDVPVVEVTRTDTGAMDDVVRLAADRARPGDTVLLAP
                     ACASMDMFISYAHRGDLFAEATHRLESERLAP"
     gene            complement(10209..11288)
                     /gene="mraY"
                     /locus_tag="VLC50_01810"
     CDS             complement(10209..11288)
                     /gene="mraY"
                     /locus_tag="VLC50_01810"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019877662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HSF26231.1"
                     /translation="MRAVLFAAGVALVVGLFGTPLAIRVLIRRGYGQLIRDDGPTSHH
                     TKRGTPTMGGIVIILATLAGYFSAHLATGRWPTASALLVLLLMAGLGLVGFLDDFIKV
                     SKQRSLGLRAKAKLAGQCLVAVTFALLALSFPDDRGLTPASSSISFIRDTGLDLFARG
                     SAIGVVGFVLWAYLMISATSNGVNLTDGLDGLATGASVMVFAAYVLIGVWQFGNSCAN
                     NPIAKCYEVRDPLDLAVLAAAVMGACFGFLWWNASPAKIFMGDTGSLALGGALAGLAV
                     VSRTELLLILLGGLFVLITVSVIVQVGFFKLTRRRVLRMAPLQHHFELKGWGEVTIVI
                     RFWIIAGLCVASGLAVFYAEWVVGV"
     gene            complement(11285..12694)
                     /gene="murF"
                     /locus_tag="VLC50_01815"
     CDS             complement(11285..12694)
                     /gene="murF"
                     /locus_tag="VLC50_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006606149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HSF26232.1"
                     /translation="MIPSTLEHLAAVTGGVVHDGDPGTVVTGPVVVDSRAAEPGSLFV
                     AMRGDRVDGHDFAQAAMAAGAVAALATRPVAVPAVVVADPLAALGALARDLLDRRPAV
                     TVTALTGSSGKTTTKDLLAQVLASTVPGAADATVATVASQNNELGLPLTVLTCDESTR
                     HLVVEMGARGAGHIDYLASIARPHVAVVLNVGSAHLGEFGSRQAIAAAKGELVAALRP
                     DGLAVLNGDDPLVRAMAERTQARVVLVGESERCQVRADDVRVDEQGRAGFTLVTPQGS
                     AAVALRVYGEHHVANALAVAAVALEHGAELPAVAAALSQAEPSSRWRMEVRQRADGLT
                     VVNDAYNANPESMRAALKALAAMAGGRRTWAVLGEMLELGPDSGTEHDAVGRLAVRLN
                     VSRVVAVGDGARLLHMGASLEGSWDNESVWVPDIETALDLLEAEVRPDDVVLVKASRA
                     IGLERVAAALLGEHAGAAS"
     gene            complement(12691..>13273)
                     /locus_tag="VLC50_01820"
     CDS             complement(12691..>13273)
                     /locus_tag="VLC50_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014376004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HSF26233.1"
                     /translation="GAFNIANAALALTATARCGVDVAAAAAGMTDCRVPGRMEAVEAG
                     QSFLAVVDFAHTPEAVATALTALRERTSGRLITVLGCGGDRDRAKRPVMGRVAAERSD
                     VLVVTDDNPRGEDPATIRDAVAEGAASVSTVELHVVGDRAAAIDQAVRTARAGDTVAL
                     LGKGHEQGQEIAGVTHPFDDRIALREAITAVHA"
BASE COUNT         1882 a         4931 c         4866 g         1594 t
ORIGIN      
        1 cggcgctcgg cctcgcgcac cttgcgggtg gcgtcggcgg ctccaccttc gacctcgttg
       61 agcttgcggg tcgcctcggc ggccgtggtc tcggcctcgg ccactgcccg ctcggacgcg
      121 ctgagccgac cgcgcaggtc gtcgttctcc cggagcagcc gagcgatctc ctgctcgacg
      181 tcatcgagga actggtcgac ctcctccatg tcgtagccct cacgcaggcg cacggacgag
      241 aactgcttgt tggcgacgtc ctcaggggtc agcggcatga gtcacctcgg gaagcgggtc
      301 ggtcaacgcc acgcgagctc ccgcgtgatc gccgacgacc acagtcggtc ggctgtcgtc
      361 acggaaccta ccccttcgac acgccccgtg accggcgcga cgcaacaccg ggactgaggc
      421 gtgtcggggc cagtccggcc aggtcagctg ggcaggccgg cgccgatgcg cgtcacgatc
      481 gggatgagga tctggatcgc caggatcagc acgagaaagg agaggtcgag cgagatccgc
      541 cccagccgca ggggcgggat cacccggcgc aacgccttca gcggcgggtc ggtgagggag
      601 tagaccacct cgcagagcac gagcacgacc ccctgcggcc gccactgacg ggcaaacatc
      661 tgcacgtagt ccaggacgag ccgggcgatc aggacgagga agtagagcca gagaagcgtg
      721 acgatgacgc cgacgagagt ccgcacaccc acatcctggc ctaccggcgg ggcaaccgaa
      781 atctagctct ggttgaagaa gccgctctcg accatcagcg ccttgtcctc ggcggtcacc
      841 tcgacgttgg ccggggagag caggaacacc ttgttggtga cccgctcgat gctgccgcgc
      901 aggccgaaga tgagcccggc cgcgaagtcg accagccgct tggcgtcgga gtcgtccatc
      961 tcggtgaggt tcatgatcac cggggtgctg tcccggaacg cctcgccgat ctgcctggcg
     1021 tcgttgtagg tgcgcgggtg gatcgtcgtg atgcggtagg ggtcggccgg acgcggccgg
     1081 gcggacaccc gggtctcgac gccggcccga cggtcggggc gcgccgccat ctcggtgacg
     1141 gtcgagcgtg gctcctcacg caccgtcggc gcctggcgcg ggtgggcgct ggggctggcg
     1201 gcctcggtcc gagccgggct cgggtcgtag ccgtcgtagc tgtcgtcgta gcgctcctcg
     1261 tcctccacga gtccgaggta gaccgccatc ttgcgcatcg cgccagccat gtcgggcgcc
     1321 ctccggtcgg tgcggacgag acgacgaacg cggtcgcggc cgtcgacggg aaactaccgg
     1381 accgcgcgac gcgagccgag caccgccgtg ccgatgcgta ggtgtgtcgc gcccgcggcg
     1441 accgcggcct cgaggtcacc gctcatgccg gccgacacga tggtcgcctg ggggtgatgc
     1501 tcacgcaccg tcaccgcgag ctcgcccagc cgcgcgaacg ccggtgccgg gtcggtgccg
     1561 agcggggcca cggccatcag gccgccgagc gcgagctggc gcgaggaggc gaccgtctcg
     1621 gccaggtcga gcaccgtgtc gggccgggct ccgccgcggc cgacgccgcc gtcgaggtcg
     1681 acctggacga ggcaggtgag tcgccggccc tctgcttccg cggcccggtc gagcgcggcc
     1741 accagccggg cccggtcgac cgagtggacg acgtcggcat atcgggccac cgaccgcgcc
     1801 ttgttggtct gcagctggcc gacgaagtgc cacacgagct ggtcgaggtc ggcgcacgca
     1861 tcgtgcttgg ctgcggcctc ctggtctcgg ttctccccta cgtcgcgcac gccgagcccc
     1921 gccagcgcgc gcacgtcgga ggccggccag ttcttggtca ccaccacgag cgtgatcgcc
     1981 gccgggtcgc gcccggcccg cgcgcacgcc gcgtcgatgc ggcggcgtac ggcggcgagg
     2041 ttggcggcca gctcgtctcg gcggctctcc tgggcgctca cgcgagccag gccagcccgg
     2101 cgtagcgccc cgtcgcaccg tcgcgccggt gcgagaacag gtcggcccgc tcggccgagc
     2161 accctggcag ccaggcgagc tcgtcacagt gcggcgccag ctgggccatc acgcccgcgc
     2221 cgacgtcgag cgcgggtgtg ccgtgggcgg tcatcgcgcg ggcctccggc gcgacggcgg
     2281 ccacctcctc gcgcatggcc agcggcacct cgtagcagcg gccgcagatc gacgggccga
     2341 cccgcccgat gatctcgcgc gcaccctgcg cgcgcatcac ctcgacgagc gcgtcgacca
     2401 cgcccgcggc cagcccacgc cgtccggcat gggcgacgcc gagcacgccg gcctgcgggt
     2461 cggccaggag caccggggtg cagtcggcga cgaggacggc cagcgccagt cccggcacgc
     2521 gggtgaccat cgcgtcggcc tccggtgccg accccgccca gccctgctcg accagcgcca
     2581 ccgagcggcc gtgcacctga cgcatgaaga ccacccggtc ggcgtccagg ccgatcgccg
     2641 ccgcgagccg cgcccggttg tcccgcacga cctgctcgcg gtcaccgacg tggtcggcca
     2701 ggttgagctc gtcgtagggc gcctggccca cgccgccgta gcggtcggtc acggccagct
     2761 cgacgaccgg ccggtccgcc gggtcggtca gtcgctcccg ccaggcgaac acgtctactt
     2821 gaggaagtct gggacgtcga ggtcgtcggt gtcgtcaaag acgatccgcc ggcctgggat
     2881 cggccgggtc ggctcgggac gtgccgtcgt cggctcgggg accgctcgtc ccgcggaggc
     2941 gggggtggtc ctgctcgggt cctcggtggc gaccgccgtc gcggccgccg cggctggcgc
     3001 agcgctgggc acggagccgg cagggccgct tacgcgggcc ggctcgggcc gggcggcggc
     3061 gtcaccggcc gtggcgtcac cgactctcgc gtcgcccgcg gtgcgcaccg ccgggcggac
     3121 cgccacgtcg tcgaagccgg ccgcgatcac cgtcacgcgc acctcgtcgc cgagcgcgtc
     3181 gtcgatgacc gcaccgaaga tgatgttggc ctcggggtgg gcggcctcgt gcacgagctt
     3241 ggcggcctcg tcgatctcga agagcccgag gtcggagccg ccggcgatcg acaggagcac
     3301 cccgtgggca ccgtcgatcg aggtctcgag cagcggtgag gagatcgcca tctgggccgc
     3361 ctccaccgcg cgggcgtcgc cgcgggccga cccgatgccc atgagcgctg agccggcgcc
     3421 ctgcatcacc gacttgacgt cggcgaagtc gaggttgatc aggccggggg tcgtgatgag
     3481 gtcggtgatg cccgagacgc cctgcagcag cacctggtcg gccgagcgga acgcgtcgag
     3541 gaccgagatc gcccggtcgc tgatcgagag cagccggtcg ttggggatca cgatgagcgt
     3601 gtcgacctcg gcgcgcagcg cggcgatgcc ggtctcggct gagccggctc gccgccgccc
     3661 ctcgaagctg aacgggcggg tcacgacgcc gatggtcagc gcgccgagcg agcgcgcgat
     3721 ccgggcgatg accggtgcgc cgcccgtgcc ggtgccaccg ccctcgccgg cggtcacgaa
     3781 gaccatgtcg gcgcccttga ggacctcttc gatctcctcg gcatggtcct ctgccgcctt
     3841 gcggcccacc tcagggtcgg cccctgctcc caggccccgg gtcagctcgc ggccgacgtc
     3901 gagcttgacg tcggcgtcgc tcatgagcag cgcctgggca tcggtgttga tcgcgatgaa
     3961 ctcgacgccc ttgagcccga cctcgatcat ccggttgacg gcgttgaccc cacccccgcc
     4021 gatgccaacg accttgatga ccgccagata gttctgcggt gctgccactg gactccccgc
     4081 cctgttgtgt cctgacccag tcggcatgtc ccgaccgcgt cgacccggaa ccacgcctgc
     4141 ccacgtggtt gcgctacccg tgcgcaaccc tcaccctcaa gtagacggtt atagttatgt
     4201 caacttcggg ctgcgcggaa cagtaggggg gccgccaggg acggtcaacg aggcgcgcgg
     4261 tgcgtctggc gtgtcgtgcc ccagatcacg tgcctcagcc tcatcgccgg gtggtcggcg
     4321 cggccggagc agagacgtcg tagacagcgg cctgctgggt ctgcatcagc acgcgcagca
     4381 cggccacctt ggccgaggcg tcagaggcgg cgccccagac gacctcgcgg tggtgggtca
     4441 gctgcagcac cacggcgtcg gcgctgcgcg cggagaccga gaccacgtgc ttggccaggt
     4501 cgtcgggcaa cccggccgcc accacagtgg ccgcgcgcag ccggtcgacc gctgcggccg
     4561 gcaccccact gatcaacggc aggcccttgg gtcgcttggc ggccgcggcg aaggcaaccc
     4621 cgtcggcgtc gacgagacgg aagccatgcc ctaccgacag caccgccgcc ggcgtgcgct
     4681 cggtcacgac gagagcgacc gtggagggcc agcgacggtc gacctcgacg tcggcgaccg
     4741 gggccagggt gcgcacccgg tcggcgatcg ccgcgcggtc gagccggacc atcgccgcgc
     4801 cgcgcggcac gtgggccgca tcgaggatcg cctgcgtcgg caggcggtct gcgccttcga
     4861 ccacgacccg gtcgaccgcc agcaccggcg agccgaggac gagccagctg cccccggcgc
     4921 cgagccccag caccgcgagc acggccagcg ccaccaccgt ggtgcgacgg cgacgccgcc
     4981 gtcgccgctg gcgggcccgt ccgaggacga ctggtggcga cggccgtcgc gccgtactca
     5041 tgccgtgcgc gcggccagcc gcgccagcac ctccggcccg atcctggtca cgtcaccggc
     5101 cccgagcgtg agcaccaggt cgccctcctc ggcgcgctcg accagccggt caggcaccgc
     5161 tgaccaggtc ggctcgaaga gcacgtggtc ctttgccagc ggtacggcgg ccgccacgag
     5221 ggcgccgctc acgccgggca ccggctcctc gcgcgcgccg tagacgtcca tcacgacgac
     5281 ctcgtcggcc agcccgagcg cctcgccgag ctcgcgggcg aagatctggg tgcggctgta
     5341 gagatgcggc tggaacgcgg cgaccacccg tcccccgccc gcgatcgtgc gcgcagcccg
     5401 caggtctgcg accagctcgc tcgggtgatg ggcatagctg tcgatcaccc ggaccccggc
     5461 agcctcgccc ttgggctcga ggcgccggcg ggtgccggtg aaccgcgcca gcccctcgcg
     5521 cagcgccccg aacggcaggc cgagggccag gcccgcgccg agcgccgcgg tcgcgttgag
     5581 cacgttgtgc cgacccggca cgcgcagcgc gacggcgccc aggcgctgcc cgtgcgcgac
     5641 gacctcgaac cgcgccgaca tgccgacggt ctccaccgcc tcgacccgca ggtcggcccc
     5701 agccgcctcg ccgtaggtga gcgtgcgcac cccgcgctcg ccggtgcgcg ccgcgagccg
     5761 ggctgccccc gggtcgtctg cgctggccac caaaaagcca tcgggcggca gcagcgccgc
     5821 gaagcggtcg aacgcctcct ggtaggcggc cggagtccca taggtgtcga ggtggtcggc
     5881 ctcgatgttg gtcaccaccg caccggtcgg ccggtaggcg aggaaggagc cgtcgctctc
     5941 gtcggcctcg gcgacgaaca cgtcgccggt gccgtcgtgc gcgttggtgc ccagctcccc
     6001 cagctcaccg ccgatcgcga acgaagggtc gaggccgcag tggcgcagcg cgacggtcag
     6061 cagcgacgtg gtcgaggtct tgccgtgcgt gccggccacc gtgaccgccc gccggcccgt
     6121 catcaccgcc gcgagcgcgg ccgctcgggg caggacccgc agcccgagcc ggcgggcctc
     6181 ggccagctcg gggttgtcgt gacggatcgc ggtggacacc acgaccgtgt cgacgccggc
     6241 gagctggtgg gccgcgtgcc cgacgtaggt gatggccccg gcggcctgca gcgcctgcag
     6301 caccgcgctc tcgcgggcgt cgctgcccga caccgtcatg ccgcgtgccc gcatgatccg
     6361 ggcgatcgcc gacatgcccg cgccgccgat gccgatgaag tggacccgcc ccagctcgtc
     6421 ggcgggcggc acctgcgccg gcagctcgac caggctcatc agcggccacc ccgaccggcg
     6481 accgcacgct cgaccatgtc ggccagccgc tcatcggcgt caggtcgccc gaacgccgcg
     6541 gcggcggcac ccatcgccgc cagccggtca gggtcggtca ccagcggtag gacgtgggag
     6601 cggagccagt cgggcgtcag cgcggcgtcc tcgaccagca ggccgccacc ggcggccacg
     6661 accggggccg cgttgagccg ctgctcgccg ttgccgaccg ggaggggcac gtagaccgcg
     6721 ggcagcccga gcgcgctgag ctcgctgacg gtgccggcgc cggcccgacc gatcaccagg
     6781 tcggcggcgg cgtaggcgag gtccatacgg tcgaggtagg gcgtcacgac gtaggccggc
     6841 ccgccgggcc gtgcgggtga ggggaactcc ttgccggccc cgctggcgtg cagcacctgg
     6901 accccggccg cgcacaggtc ggccgccacc gcaggcacca cctcgttgag ccgggtcgcg
     6961 cccagcgagc cgccgaccac gagcacggtg ggacgcgtcg gggcgagacc cagcgcggcc
     7021 cgggcgcccg ggcgggcggc gggccggtcg agcaaggtga tctcgcggcg cagcggcagc
     7081 ccagtgcgca gcgcatgagg cagccgggtg ccggggaagg tggtcgcgac ccacggggtc
     7141 agccgtgccc ccagccggtt ggccagtccg ggtcggctgt tggcctcgtg gaccacgatc
     7201 ggcagccggc gccgccgggc ggccaggtag gcgggggtgg agacgtagcc gccgaagccg
     7261 accacgacct cggccccgac ctcctcgatc gcctgccccg cagctcggac ggcgtcgacg
     7321 agacgcgccg gcaggcgcag gaggtcggcc gagggacggc gcgggagcgg cacccgcggc
     7381 acgaacgcca gccggtagcc gcgctcgggc accagccggg actccagccc ctcggcggtg
     7441 cccagcgcga gcaccgcgac atcgggatcg cgacggacca aggtgtcggc gagcgccagc
     7501 agcggcgcga cgtggccggt cgacccaccg ccggccagca ggaccctcat gccgctcacc
     7561 gcctgccctg ccgcgccagc cggcgcaacg gtgccgggcg ggaccgcagc acggcggccg
     7621 cctccggtgt cgagcgggcg aacgagagca gcatgcccag ccccaggagc acggtgatca
     7681 gcgaggagcc gccgtaggag accagcggca gggagacgcc gacgatcggg agcagcccga
     7741 gcaccgcgcc gatgttgacc accgcctgga tcatgatcca ggcggtgacc ccgccggcgg
     7801 ccaaccggac gaacaggtcg ttggagcgta cggcgacccg gaacccgccg taggccagcg
     7861 cgatgaacag cagcagcacg gtgagcgtgc cgatgagccc gagctcctcg ccgatgatcg
     7921 cgaagatgaa gtcggtgtgc gcctccggca gggcacccca cttctgccgg ctggcgccga
     7981 gcccgaggcc ccaccagccg ccggtggcca gcgcgtagtg accgtgcacc gcctgccagc
     8041 cggcgtcgag gtagtcctgg ctgggcgtcc gccaggagcc cagccgcttc atccggttgg
     8101 ggctgctgac gacgagcagg gccacgatcg tcccgaacgc cagcaggctg ccggcgaaca
     8161 gccgcagcgg tgctccgacg aggaagagca gggctccgac gatcgccatg atgacgagca
     8221 ccgtgcccag gtcgttgccc agcatcacca gggccagcac gagaacggac accgggacca
     8281 gcggcacgag caggtgcttc cactgggaga ggagccggtg cttgcgggcc agcaggtcgg
     8341 cgccccagac gatgagcgcc agcttggcca gctccgaggg ctgcatgcgc atgccggcga
     8401 ggtcgagcca gttgcggttg cccttgacct cgaccccgac gagcgggacc agcgcgagca
     8461 gggcgagggc ggccagtagc ccgatcggcg ccatccgccg gtagaaccgc accggcagcc
     8521 gtgacgcggc ccacatgagc ggcagggcca ccgccaccca catcgcctgg cgctggaaga
     8581 tggtgaacga cgacttggtc tgcacgtagg actcgacgct cgaggccgac agcaccatga
     8641 ccagcccgag caggacgagc agggcggtcg agccgagcac caggtagtag gaggccagcg
     8701 gccggtcgag cgcccgggtc ggctcgcggg cggcctcccg caggcgcggg gcggcagcag
     8761 tcgtcacggg gccagccgct cggactcgag ccggtgggtg gcctcggcga acaggtcgcc
     8821 ccggtgggca tagctgatga acatgtccat cgacgcgcag gccggggcca gcagcacggt
     8881 gtcgcccggc cgggcacggt cggcagccag gcgtacgacg tcgtccatcg ccccagtgtc
     8941 ggtgcgcgtg acctccacga ccggcacatc gggggcgtgt ctcgccagag ccgccctgat
     9001 cacgtcgcgg tcacgtccga gcaggaccac cgcccgcagc gccgtcccgg cgcgcgcgac
     9061 gaggtcgtcg aaggtggcgc ccttggccag cccaccggcg atccagacga ccgagtcata
     9121 ggcggccagc gacgccgcgg ccgcatgcgg gttggtcgcc ttggagtcgt cgacgaagcc
     9181 gacgccgtcg atcgtggcca cgtgggcgat ccggtgcgcg ccgggccgga aagagcgcag
     9241 cccgtcgcgg accgcgtctg caccgacccc ggcggcacgg gccagcgcgg ccgccgccag
     9301 cgcgttggcg acgttgtgcg gcgcggccgg cgtgatgtcg gcgaccgcgg ccagctcgac
     9361 cggctccgag gccgagggcc cgaaggcgcg atcgaggagc accccctgct cgacgccgag
     9421 catgcccgca cccgggacgc ctcgggtgaa ccccacggcg cgggcgccat cagccccggt
     9481 gcggggcagg agcgcctcgg tggcggcgtc ctccacgttg tagacggccg ccgcctgcgc
     9541 ccatcgcagc agcttgccct tggccgcccc gtaggcatcc atcgacccgt gccagtcgat
     9601 gtggtcgggc gcgaggttga gcaccgcgcc cgccagcggc gccagcgtgt gggcccagtg
     9661 cagctcaaag ctggacagct cgacggcgag cacgtcgtag ggggtcggct cgagcaccgt
     9721 ggccacgagc gaggtgccga cgttgccggc ggccagcgcc cggcgcccgt cggccagcag
     9781 catcgcttcg agcatgcgca ccgtggtcgt cttgccgttg gtcccggtca gggccagcca
     9841 ctgctggccg gccgggcgca gccgccaggc cagctcgacg tcgccccaga cgggcacgcc
     9901 gcgcccggcg gcggcccgca gcagcggttg gtcgggccgc cagcccggag aggtgatcac
     9961 gaggtcggtg ccggcgggca gctcacggct ggccgcctcg ccgaggctca cccgcacccc
    10021 gacctcacgc agcgccgcgg ccgcctgctg gtgcctctcg ctggtgccgg cgtcgacgac
    10081 ctgcaggtcg gccacgccca ggcccgccaa cgcgatggcc accgccttgc ccgagacacc
    10141 cagcccggtg acgacgacgt gacgcgaggg ccacggcagc tcctgccccg gcccggtcag
    10201 ccgaccgctc atacgccgac gacccactcg gcgtagaaca ccgccaggcc ggacgcgacg
    10261 cagagcccgg cgatgatcca gaaccggatg acgatcgtga cctcgcccca gcccttgagc
    10321 tcgaagtggt gctgcagggg cgccatccgc agcacccgcc ggcgggtgag cttgaagaac
    10381 cccacttgca cgatcacgga cacggtgatg aggacgaaca gcccgccgag caggatcagc
    10441 aacagctcgg tgcgcgagac gacggccagc ccggccagcg cgccgccgag ggccaaggac
    10501 ccggtgtcac ccatgaagat cttggcgggc gacgcgttcc accacaggaa gccgaagcag
    10561 gcgcccatca cggcggccgc gagcaccgcc aggtcgagcg ggtctcgcac ctcgtagcac
    10621 ttggcgatcg ggttgttggc gcagctgttg ccgaactgcc acaccccgat gagcacgtag
    10681 gcggcgaaca ccatgaccga ggccccggtg gccagcccgt cgaggccgtc ggtgaggttc
    10741 accccgttgg aggtggccga gatcatgaga taggcccaca gcacgaaccc gacgaccccg
    10801 atcgcgctgc cccgggcgaa caggtcgaga ccggtgtcgc ggatgaacga gatgctcgac
    10861 gaggccggcg tcaggccccg gtcgtcggga aagctcaagg ccagcaaggc gaaggtgacc
    10921 gcgacgaggc actgaccggc cagcttggcc ttggcccgca ggcccaggct gcgttgcttg
    10981 gagaccttga tgaagtcgtc gaggaagccc accagcccga gcccggccat gagcagcagc
    11041 acgagcagcg ccgaggcggt gggccagcgc cccgtggcca ggtgcgcgga gaagtagccg
    11101 gccagcgtgg ccaggatgat gacgatgccg cccatcgtcg gggtgccgcg cttggtgtgg
    11161 tgcgaggtgg gcccgtcgtc gcggatcagc tggccgtagc cgcggcggat cagcacccgg
    11221 atcgccagag gggtgccgaa cagcccgacg acgagtgcca ccccagcggc gaagagcacc
    11281 gccctcacga ggccgccccc gcgtgctcgc cgagaagcgc ggccgcgact cgctcgaggc
    11341 cgatcgcccg cgaggccttg accagcacga cgtcgtcggg tcggacctcg gcctcgagca
    11401 ggtcgagcgc ggtctcgatg tcggggaccc aaaccgactc gttgtcccac gagccctcga
    11461 ggctggcccc catgtgcagc agccgggcgc cgtcgccgac cgccacgacc cgcgagacgt
    11521 tcaggcgtac ggcgagccga ccgaccgcgt cgtgctcggt gccggagtcg gggcccagct
    11581 cgagcatctc tcccagcacc gcccaggtgc gccgaccgcc cgccatcgcc gccagcgcct
    11641 tcaacgcggc ccgcatcgac tcagggttgg cgttgtaggc gtcgttgacg acggtgagcc
    11701 cgtcggcgcg ctggcggacc tccatccgcc atcggctgct cggctcggcc tgcgagagcg
    11761 cggcggccac cgcgggcagc tcggcgccgt gctcgagggc gaccgcggcg accgccaggg
    11821 cgttggcgac gtggtgctcg ccgtagaccc gcagcgcgac ggcggcgctg ccctgcggtg
    11881 tcaccagggt gaacccggcc cggccctgct cgtcgacccg cacgtcgtcg gcgcggacct
    11941 ggcaccgctc gctctccccc accaggacca cccgggcctg cgtgcgctcg gccatcgccc
    12001 gcacgagtgg gtcgtccccg ttgagcacgg ccagcccgtc ggggcgcagg gcggcgacca
    12061 gctcgccctt ggccgcggcg atggcctggc gggagccgaa ctcacccagg tgggccgagc
    12121 cgacgttgag cacgaccgcc acgtgggggc gcgcgatgct cgcgaggtag tcgatgtgcc
    12181 cggcgccccg cgcgcccatc tcgacgacca ggtgccgggt ggactcgtca caggtcagca
    12241 cggtcaacgg gagcccgagc tcgttgttct gcgaggcgac ggtcgccacc gtcgcgtccg
    12301 ccgccccggg caccgtcgag gcgagcacct gggcgagcag gtccttggtg gtcgtcttgc
    12361 cggatgaccc ggtcaacgcg gtgaccgtga cggctgggcg ccggtcgagc aggtcgcgcg
    12421 cgagggcgcc gagagcggcc agcgggtcgg cgacgacgac cgcgggcaca gcgaccgggc
    12481 gggtggccag cgcggccacc gctccggccg ccatcgcggc ctgggcgaag tcgtggccgt
    12541 cgacgcggtc gccccgcatc gcgacgaaca gcgagccggg ctcggccgca cgcgagtcga
    12601 cgacgaccgg gccggtcacc accgtgccgg ggtcgccgtc gtgcaccacg cccccggtga
    12661 ccgcagccag gtgctcgagc gtcgaaggga tcacgcgtgc accgccgtga tcgcctctcg
    12721 aagtgcgatc cggtcgtcga acgggtgggt caccccagcg atctcctgtc cttgctcgtg
    12781 ccccttgccc agcagggcaa cggtgtcacc ggcgcgtgcg gtgcgcacgg cctggtcgat
    12841 ggcggccgca cggtcaccga ccacgtgcag ctcgacggtg ctgaccgaag cggccccctc
    12901 ggcgaccgcg tcccggatcg tcgccgggtc ctccccgcgc gggttgtcgt cggtcaccac
    12961 gaggacgtcg gagcgctcgg cggcgacgcg gcccatgacc gggcgcttgg cccggtcgcg
    13021 gtcaccaccg cagccgagca cggtgatcag gcgcccgctg gtgcgctcgc gcagcgcggt
    13081 cagcgcggtg gccaccgcct cgggggtgtg cgcgaagtcg acgacggcca ggaacgactg
    13141 gccggcctcg accgcctcca tgcggcccgg cacccggcag tcggtcatcc cggcggcggc
    13201 cgccgccacg tcgacaccgc accgggccgt cgcggtgagc gccagcgcgg cgttggcgat
    13261 gttgaacgcg ccg
//
LOCUS       DATIBB010000051         2008 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_1339566, whole genome shotgun sequence.
ACCESSION   DATIBB010000051 DATIBB010000000
VERSION     DATIBB010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2008
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1339566"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1077)
                     /locus_tag="VLC50_01825"
     CDS             complement(<1..1077)
                     /locus_tag="VLC50_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020673636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HSF26234.1"
                     /translation="MNDQAEADPDVLIVGGGIGGLSNALGLARAGHRVRVLESAPEFS
                     EIGAGLQLAPNANRILRDWGLLDEVAAAGFRPRRLVLKDAIDGGELTHLPIDDAFEAR
                     YGTPYYVIHRSDLLDILVRACKQAGVELRTGAHVEDVSTEPDGATVTVASGPAQRAAV
                     VLAADGLWSTLRRRFSDDEPVCSGFAAYRGAVPMEEMADRLDELSVEDMVVYFGPGCH
                     FVQYPLRGGTMFNQVAVFRSPSFARGEAEWGGPEELDDAFAPCCHAVRHGLDKLWRDR
                     HWPMFDRVPITHWVDGRVALTGDAAHPMLQYLAQGACQAIEDAGVFSSYADPAGAWPG
                     SWEGALSSYAADRVARAGRVQTLAR"
     gene            complement(1143..1850)
                     /locus_tag="VLC50_01830"
     CDS             complement(1143..1850)
                     /locus_tag="VLC50_01830"
                     /inference="COORDINATES: protein motif:HMM:TIGR03083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase family
                     mycothiol-dependent enzyme"
                     /protein_id="HSF26235.1"
                     /translation="MNAPLETVRAWMDLGTAKVEDVVRRLSDADLDADTALPGWTRRH
                     LLAHLGFNAEALRHLASWAATGVRNPMYASAEARNAEIEEGARWAPARLREFVVETDA
                     ALRADLDALPAHCWDAQVETAQGRIVAATEIPWMRTREVAVHAVDLAAGVGFADLPDE
                     LCLALIDDVMGRRASRGTDPAFEVSVAGQRWTVPGEGEPAQVTGSPAALAQWLTGRGA
                     AGLTTPPDQVPALPRWL"
     gene            complement(1847..>2008)
                     /locus_tag="VLC50_01835"
     CDS             complement(1847..>2008)
                     /locus_tag="VLC50_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017983175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HSF26236.1"
                     /translation="LSPGDVIATGTPGGVGHARKPPRYLTDGSRLVTEISGIGRLDNV
                     ARPEVLAAR"
BASE COUNT          265 a          764 c          732 g          247 t
ORIGIN      
        1 gcgggccagc gtctgcaccc gcccggcgcg ggcgacccgg tcggcggcgt acgacgagag
       61 cgcgccctcc cacgaccccg gccaagcccc ggccggatcg gcgtacgacg agaacacccc
      121 ggcgtcctcg atcgcctggc aggcaccctg ggcgaggtac tgcagcatcg ggtgcgccgc
      181 gtcgccggtc agcgcgaccc gcccgtcgac ccagtgggtg atcggcaccc ggtcgaacat
      241 cggccagtgc cggtcgcgcc acagcttgtc gagcccgtga cggacggcat ggcagcacgg
      301 ggcgaacgcg tcgtcgagct cctccggccc gccccactcg gcctcgccgc gggcgaagga
      361 cggcgagcgg aacaccgcga cctggttgaa catcgtgccg ccgcgcaacg ggtactgcac
      421 gaaatggcag cccggcccga agtagacgac catgtcctcg accgagagct cgtcgagccg
      481 gtcggccatc tcctccatcg gtacggcgcc ccggtaggcg gcgaagccgg agcagaccgg
      541 ctcgtcgtcg ctgaaccggc gccgcagcgt cgaccacagc ccgtcggcgg ccagcaccac
      601 cgcggcccgc tgagccggac cgctcgccac cgtcacggtc gcgccgtcag gctcggtgct
      661 cacgtcctcg acgtgggcgc cggtgcgcag ctcgaccccg gcctgcttgc acgcgcgcac
      721 caggatgtcg agcaggtcgc tgcggtggat gacgtagtag ggcgtgccgt agcgcgcctc
      781 gaaggcgtcg tcgatcggca ggtgggtcag ctcgccgccg tcgatcgcgt ccttgagcac
      841 cagccgcctg ggccggaacc cggcggcggc cacctcgtcg agcaggcccc agtcgcgcag
      901 gatccggttg gcgttggggg ccagctgcag cccggcgccg atctcggaga actccggcgc
      961 cgactcgagc acccgcaccc ggtggccggc ccgggccagc ccgagcgcgt tggacaggcc
     1021 gccgatcccg ccgccgacga tgaggacgtc ggggtcggcc tcggcctggt cgttcatgaa
     1081 tgcctccgtt gcatccgtca agcagtcacg cagtagggct tcgtcacgcg gcggggcgcc
     1141 ggctagagcc agcgggggag cgcgggcacc tggtcgggtg gggtggtcag cccggcggcg
     1201 ccgcgaccgg tcagccactg ggccagcgcg gccggcgagc cggtcacctg ggccggctcc
     1261 ccctcaccgg gcaccgtcca gcgctgcccg gccacgctca cctcgaacgc cgggtcggtg
     1321 ccgcggctgg cccgtcggcc catcacgtcg tcgatcaggg ccaggcacag ctcgtcgggc
     1381 aggtcggcga acccgacccc ggcggccagg tcgaccgcgt gcacggccac ctcgcgggtg
     1441 cgcatccacg ggatctcggt ggccgcgaca atgcgcccct gcgcggtctc cacctgggcg
     1501 tcccaacaat gcgccggcag cgcgtcgagg tcggcccgca gcgccgcgtc ggtctccacg
     1561 acgaactcac gcagccgcgc cggcgcccac cgcgcgccct cctcgatctc ggcgttgcgc
     1621 gcctcggccg aggcatacat cgggttgcgc accccggtcg cggcccacga cgccaggtgc
     1681 cgcaaggcct ccgcgttgaa gccgaggtgg gcgagcaggt gccggcgggt ccagccgggc
     1741 agcgcggtgt cggcgtcgag gtcggcatcg gacagccgtc gtacgacgtc ctcgaccttc
     1801 gccgtaccca ggtccatcca ggcccggacc gtctcgagcg gtgcgttcac cgggcggcca
     1861 ggacttccgg gcgggcgaca ttgtcgagcc ggccgatccc gctgatctcg gtgaccagcc
     1921 gggagccgtc ggtgaggtag cgcggtggct tgcgggcgtg cccgaccccg cccggcgtgc
     1981 cggtggcgat cacgtcgccg ggggagag
//
LOCUS       DATIBB010000052         5787 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_551301, whole genome shotgun sequence.
ACCESSION   DATIBB010000052 DATIBB010000000
VERSION     DATIBB010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 5787)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5787)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5787
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_551301"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..998)
                     /locus_tag="VLC50_01840"
     CDS             complement(<1..998)
                     /locus_tag="VLC50_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007270412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation acetate symporter"
                     /protein_id="HSF26237.1"
                     /translation="MVLAAEAARGAGNRGLTITLFGAVVLVTLYITYWASKQNKTAAD
                     YYAGGRSFSGFQNGLAISGDYMSAASFLGISGAIALNGYDGFLYSIGFLVAWLVALLL
                     VAELLRNTGKFTMADVLSFRLRQRPVRMAAAISTLAVSYFYLLAQMAGAGGLVSLLLG
                     IEDRVGQSIIIAIVGVIMVAYVLIGGMKGTTWVQIIKAVLLIAGALLMTVWVLSLFGF
                     NLSNLLGAAVTESGKDVLSPGLQYGKSDITKLDFISLSMALVLGTAGLPHVLMRFYTV
                     PSSKEARRSVVWAIWLIGLFYLFSLVLGYGAGALVGPTEIKAAPGGANSAAPLLAF"
     gene            complement(1004..1321)
                     /locus_tag="VLC50_01845"
     CDS             complement(1004..1321)
                     /locus_tag="VLC50_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007381098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HSF26238.1"
                     /translation="MELLASQKALHDSPEFAQLRSRFRRFVFPVTVGFLVWYFAYVLA
                     ANYAHDFMSTNIVGNINVALVWGLLQFVSTFGIAWWYSKKAASDFDPLATELRERFEG
                     AAK"
     gene            complement(1569..3320)
                     /locus_tag="VLC50_01850"
     CDS             complement(1569..3320)
                     /locus_tag="VLC50_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012853495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation acetate symporter"
                     /protein_id="HSF26239.1"
                     /translation="MNAVYGVVAVLAVTIATIGIGAFGLRFSRTTSDFYVASRSVSPV
                     WNASAIGGEYLSAASFLGVAGLILAFGADMLWFPVGYTAGYLVMLVLVAAPLRRSGAY
                     TLPDFAEARLESRPVRRIASVLVVLIGWLYLLPQLEGAGLTLHTVTGAPAWSGGALVA
                     FVVILNVISGGMRSITFVQAFQYWLKLTAICLPVIFLLLAWQGDGRPSPADEARPTFH
                     QATTVDVGRTITVRVDTPVVVRVDGVIDDLRHDEGTSVALTRGTHVIRSGTTLDFDAG
                     DEVPHVAEISAASHGDWLLPLSGAGGRDHPLLATYSLVLATFFGTMGLPHVLVRFYTN
                     PDGRAARRTTLIVLVLLSAFYLMPTIYGALGRLYAPDLLLTGRTDAAVLLLPGRLVGG
                     ALGDLLGALVTAGAFAAFLSTSSGLAVSVAGVLSQDLLRGSVRDFRLATGLAIIVPLA
                     LSTLAAALSVATVVGLAFAVAASSFCPLLVLGIWWRRLTDVGAAAGLLVGGGLATAAV
                     VTTIVMGPQSGWLGTLLAQPAALTVPVAFAVMVLVSLLTPRRVARDVARTMVRLHAPE
                     SVQLDRGDFHPERRRRP"
     gene            complement(3317..3697)
                     /locus_tag="VLC50_01855"
     CDS             complement(3317..3697)
                     /locus_tag="VLC50_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019873955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF26240.1"
                     /translation="MSELGRPRRVRVTSPRTRQARPPTWVAATREIDEQSSIGQVYMR
                     SLIRSQLRLSLAVCVVFGVVLGGLPLLLELVPSLRRVHMLGLPLPWVVLGAVVYPVII
                     FGGWLYVRAAERTERDFIELVQQQ"
     gene            complement(3694..4458)
                     /locus_tag="VLC50_01860"
     CDS             complement(3694..4458)
                     /locus_tag="VLC50_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019873954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HSF26241.1"
                     /translation="MLSVLVVDDELPALEELGWLLRQDPRVGSVRLAQDGATALRLLD
                     EQQVDALFLDIRMPGLDGLALARVLARFKEAPQVVFVTAYDDHAVDAFELDAADYLLK
                     PVHPERLAEAVRRVVVATEPGGDGPDPGGPADPLPEETIPVELGGVTRFVARSDVRWV
                     EAAGDYARLHTADGSSHLVRVPLTTLEERWGAAGFVRIHRSTLVALTHIDELRLDTGH
                     CTVRVGDEVLQVSRRHTRELRDLLVRRARPVSSEPR"
     gene            complement(4539..5672)
                     /locus_tag="VLC50_01865"
     CDS             complement(4539..5672)
                     /locus_tag="VLC50_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020540565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HSF26242.1"
                     /translation="MGAWRALRPHGRRNDLGSPADRATFATLHTASLASPPLRAGLTA
                     AGAARASKHLRALLGTSAVALTDGESVLAWDGAGERHAADAVAHGRTVMETGRTEVLG
                     PVVACYRASCPVRSAVIAPITAQDRVIGTLAAYGEDASAGLVRATQEVARWVSSQVEL
                     AELDASRTRLMEAEVRALRAQISPHFIYNSLTAIASFVRTDPERARELLLEFADFTRY
                     SFRRHGDFTTLAEELRSIDRYLLLERARFGDRLQVTLRVAPEVLPVAVPFLCLQPIVE
                     NAVRHGLEGRPGQGRVSITAEDAGSEAHIFVEDDGVGIDPARVREALAGASGGAHVGL
                     GNVDARLRQVYGDEFGLIVETAPGAGTKVSLRIPKFHPGVHAS"
BASE COUNT          936 a         2009 c         2162 g          680 t
ORIGIN      
        1 tcgaacgcca gcagcggcgc ggccgagttc gccccgccgg gagccgcctt gatctccgtc
       61 gggccgacca gggcgccggc gccgtagccc agcaccaggc tgaacaggta gaacaggccg
      121 atcagccaga tcgcccagac caccgagcgg cgcgcctcct tcgacgaggg cacggtgtag
      181 aagcgcatca gcacgtgcgg caggcctgcg gtgccgagca ccagggccat cgacagcgag
      241 atgaagtcca gcttcgtgat gtccgacttg ccgtactgca gcccggggga gagcacgtcc
      301 ttgccggact cggtcaccgc ggctccgagg aggttgctga ggttgaagcc gaacaggctg
      361 aggacccaga ccgtcatcag cagggcgccg gcgatcagca ggacggcctt gatgatctgc
      421 acccaggtgg tccccttcat gccgccgatg aggacgtacg cgaccatgat cacgccgacg
      481 atggcgatga tgatggactg gccgacgcgg tcctcgatgc cgagcagcag cgagacgagt
      541 ccgccggcgc cggccatctg ggccagcagg tagaagtacg acaccgcgag ggtcgagatg
      601 gcggccgcca tgcgcaccgg gcgctgccgc agccggaagc tcagcacgtc ggccatggtg
      661 aacttgcctg tgttccggag cagctcggcc acgagcagca gcgccacaag ccaggcgacg
      721 aggaagccga tcgagtagag gaagccgtcg tagccgttga gcgcgatggc cccagagatg
      781 ccgaggaacg acgccgccga catgtagtcg ccggagatgg ccaggccgtt ctggaagccg
      841 gagaacgagc ggccaccggc gtagtagtcg gccgccgtct tgttctgctt gctggcccag
      901 taggtgatgt agagggtgac gaggacgacc gccccgaaca gggtgatcgt caggccgcgg
      961 ttgccggccc ctcgcgccgc ctcggcggcg agcaccatgg tgttcacttg gcggctcctt
     1021 cgaagcgctc gcgcagctcg gtggccagcg ggtcgaagtc actcgccgcc ttcttcgagt
     1081 accaccaggc gatgccgaac gtcgagacga actgcagcaa gccccagacg agcgcgacgt
     1141 tgatgttgcc gacgatgttg gtggacatga agtcgtgcgc gtagttggcc gcgagcacgt
     1201 aggcgaagta ccagaccaag aagccgacag tcaccgggaa gacgaaccgg cggaaccgcg
     1261 agcgcagctg ggcgaactcg gggctgtcgt ggagagcctt ctggctggcg agcagctcga
     1321 tggcttcctc gtcgtgtggc ggaccgccgc cgtcctgaac agtgctcact aatcctcctc
     1381 aaagaggtcg cgggcgcatg tggcccatgt gatcggcgtc actgtgacgt tcgcggtaac
     1441 ccccgcaaaa ggggcgacac cccggaggtg gtccagcggt cggcgctggt cgccgtacgg
     1501 caggcgcact gccgtacggc gtacgacgag cggagcgctg ggcgggacga gcggcgctga
     1561 gggcgccgtc agggccggcg acggcgctcg gggtggaagt caccgcggtc gagctgcacc
     1621 gactcggggg cgtgcagccg caccatcgtg cgggccacgt cacgggccac ccggcgcggg
     1681 gtcagcagcg agaccagcac catgacggcg aacgccaccg gcacggtcag cgcggccggc
     1741 tgggcgagca gcgtgccgag ccagcccgac tgcggcccca tcacgatcgt cgtcaccacc
     1801 gcggcggtgg ccagcccgcc gccgacgagc agcccggccg ccgccccgac gtcggtgagc
     1861 cggcgccacc agatgccgag cacgagcaac gggcagaacg aggacgccgc caccgcgaac
     1921 gccagcccga cgacggtggc caccgacagc gcggcggcca gggtggacag cgccagcggc
     1981 acgatgatcg ccagaccggt ggccagccgg aagtcgcgca cactgccccg cagcaggtcc
     2041 tgcgagagca cgccggccac cgacacggcc aacccgctcg acgtggacag gaaggcggcg
     2101 aacgcgccgg cggtcaccag cgcgccgagc aggtcgccga gcgcgccacc gacgaggcgg
     2161 ccgggcagca gcagcaccgc cgcgtcggtg cgcccggtga gcaggaggtc gggcgcgtag
     2221 agccggccga gcgcgccgta gatggtcggc atgaggtaga acgccgagag caggaccagc
     2281 acgatcagcg tcgtacgacg ggcggcccgg ccgtcggggt tggtgtagaa gcgcaccagc
     2341 acgtgcggca ggcccatcgt gccgaagaag gtcgccagca cgagcgagta ggtggccagc
     2401 agcgggtggt cgcgcccgcc cgcgcccgac agtggcagca gccagtcgcc gtggctggcc
     2461 gcgctgatct cggcgacgtg cggcacctcg tcgccggcgt cgaagtcgag ggtcgtgccc
     2521 gacctgatga cgtgggtgcc gcgggtgagg gccaccgagg tgccctcgtc gtggcgcagg
     2581 tcgtcgatga cgccgtcgac ccgcacgacc accggggtgt ccacccgcac ggtgatggtg
     2641 cgcccgacgt cgacggtcgt ggcctggtgg aacgtcggtc gtgcctcgtc ggccgggctg
     2701 ggccgcccgt cgccctgcca ggccagcagc aggaagatca ccggaaggca gatcgcggtg
     2761 agcttgagcc agtactggaa cgcctgcacg aacgtgatgc tgcgcatccc gccggagatc
     2821 acgttgagga tgacgacgaa ggccaccagc gcgccaccgc tccaagccgg tgccccggtg
     2881 acggtgtgca gcgtcagccc ggcgccctcg agctggggga gcaggtagag ccagccgatg
     2941 agcaccacga gcacgctggc gatccggcgc accgggcgcg actcgagccg ggcctcggcg
     3001 aagtcgggca gcgtgtaggc ccctgaccgg cgcagcggcg cggccacgag cacgagcatc
     3061 acgaggtagc cggcggtgta gccgaccggg aaccacagca tgtcggcgcc gaaggcgagg
     3121 atcagcccgg cgacgccgag gaacgaggcg gccgacagat actcgccgcc gatcgcggag
     3181 gcgttccaca ccggcgagac cgaccgggag gcgacgtaga agtcgctcgt cgtgcgcgag
     3241 aaccgcagcc cgaaggcgcc gatgccgatg gtggcgatgg tgacggcgag gaccgcgacg
     3301 acgccgtaca ccgcgttcac tgctgctgca ccagctcgat gaagtcgcgc tcggtgcgct
     3361 cggcggcccg cacgtagagc cagccgccga agatgatcac ggggtagacg accgcgccga
     3421 gcaccaccca gggcagcggg agaccgagca tgtggaccct gcgcaggctg ggcaccagct
     3481 cgagcagcag cggcaggccg ccgagcacga caccgaagac cacgcacacg gccagtgaga
     3541 gccgcagctg gctgcggatc agcgagcgca tgtagacctg cccgatgctg ctctgctcgt
     3601 cgatctcgcg ggtggccgcg acccaggtcg gcgggcgcgc ctgccgggtg cgcggggagg
     3661 tgacccgcac ccgccggggg cgcccgagct cgctcaccgg ggttcgctcg agaccggccg
     3721 ggcccggcgg acgagcaggt cacgcagctc gcgggtgtgc cggcggctga cctgcagcac
     3781 ctcgtcgccg acgcgcaccg tgcagtggcc ggtgtccagc cgcagctcgt cgatgtgggt
     3841 gagcgcgacc agcgtgctgc ggtggatgcg cacgaagccg gcggcgcccc atcgctcctc
     3901 gagcgtggtg agcgggaccc gcacgaggtg gctggagccg tcggcggtgt gcagccgggc
     3961 atagtcgccg gccgcctcga cccagcgcac gtccgaacgg gcgacgaagc gggtcacccc
     4021 gcccagctcg accgggatgg tctcctcggg cagcgggtcg gccggcccgc cggggtcggg
     4081 cccgtcgccg cccggctcgg tggccacgac gacccggcgt accgcctcgg ccagccgttc
     4141 ggggtggacc ggcttgagca ggtagtcggc cgcgtcgagc tcgaaggcgt ccacggcgtg
     4201 gtcgtcgtag gcggtgacga acaccacctg gggggcctcc ttgaaccggg ccagcaccct
     4261 ggccagcgcc agcccgtcga ggccgggcat tcggatgtcg aggaagaggg cgtcgacctg
     4321 ctgctcgtcg agcagccgca gcgcggtggc gccgtcctgg gccagccgca ccgacccgac
     4381 ccgaggatcc tggcggagca gccagccgag ctcttcgagc gcgggcagct cgtcatcgac
     4441 gacgaggacg ctgagcatgg cgggagggta cgggaggggt gacctccggc acatcacctc
     4501 tccgggatca aaatccgatt tgtcgcatat cgggggcatc agctggcgtg cacgcccggg
     4561 tggaacttcg ggatccgcag gctgaccttg gtgccggccc cgggcgcggt ctcgacgatc
     4621 aggccgaact cgtcgccgta gacctgccgc agccgcgcgt cgacgttgcc cagcccgacg
     4681 tgcgccccgc cggaggcccc ggccagcgcc tcgcgcaccc gggccgggtc gatgcccacg
     4741 ccgtcgtcct ccacgaagat gtgggcctcg ctgccagcgt cctcggcggt gatcgacact
     4801 cggccctgcc cgggccgacc ctccagcccg tgccggacgg cgttctcgac gatcggttgc
     4861 aggcacagga acggtacggc gaccggcagc acctccggcg cgacccgcag ggtcacctgc
     4921 agccggtcgc cgaagcgggc ccgctcgagc agcaggtagc ggtcgatcga gcgcagctcc
     4981 tcggccagcg tggtgaagtc gccgtgccgg cggaacgagt agcgggtgaa gtcggcgaac
     5041 tccagcagca gctcgcgggc ccgctcgggg tcggtgcgca cgaacgacgc gatcgcggtc
     5101 agcgagttgt agatgaagtg cggggagatc tgcgcgcgca gggcgcggac ctcggcctcc
     5161 atgagccggg tccgcgaggc gtcgagctcg gccagctcga cctggctgga tacccatcgg
     5221 gccacctcct gggtggcccg caccagcccg gccgaggcgt cctcgccgta ggccgccagc
     5281 gtgccgatca cccggtcctg ggcggtgatc ggggcgatca ccgcgctgcg caccgggcag
     5341 gacgcgcggt agcaggcgac gacgggccca agcacctcgg tgcgcccggt ctccatcacg
     5401 gtgcgcccgt gcgccaccgc gtcggcggcg tgccgctcgc cggcgccgtc ccaggccagc
     5461 accgactcgc cgtcggtcag cgccaccgcc gaggtgccga gcagcgcccg caggtgcttg
     5521 gacgcccgag cggcccccgc cgcggtgagc ccggcacgca gcggcgggga ggcgagagag
     5581 gcggtgtgca gggtggcgaa ggtggcccgg tcggcggggg agccgaggtc gttgcggcgc
     5641 ccgtgcgggc gcagcgcccg ccaggcgccc atcgtcgtga cgaccacgag aagcaccgcg
     5701 acggtgccgg tcagtggctc gagcacccgc gcaccgtagc gcccgtccgc gccgggtccc
     5761 gaccgccata gggtcggcga cgtgcca
//
LOCUS       DATIBB010000053        20397 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_683733, whole genome shotgun sequence.
ACCESSION   DATIBB010000053 DATIBB010000000
VERSION     DATIBB010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 20397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20397
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_683733"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..512)
                     /locus_tag="VLC50_01870"
     CDS             complement(<1..512)
                     /locus_tag="VLC50_01870"
                     /inference="COORDINATES: protein motif:HMM:NF014741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HSF26243.1"
                     /translation="MDGASSTNWVGSGHPLLAGLHLTTKGLTTAAGPGRDRVSLLSDE
                     DTRAGLDAVAAAEARLVAIRASLLAHAEVRRVREQLKARTTASWLADVARISGATARE
                     HVKVAGMLDGCPEAAGLLAAGAATLAHARIVADTLKDIDKLGTVDVATRAAAVAFLTK
                     QARLLDPVAL"
     gene            901..2715
                     /locus_tag="VLC50_01875"
     CDS             901..2715
                     /locus_tag="VLC50_01875"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020578783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HSF26244.1"
                     /translation="MSAETVAAPTTHQPLIDWVAEVAALTEPDRVVWADGSDEEWRRL
                     TDELCETGVLVRLDESRKPNSFWARTDPSDVARVEGRTFICSEREEDAGPTNNWMAPA
                     EMKATMTELFRGSMHGRTMYVIPFCMGPLSAERPKLGVEITDSAYVAASMRIMTRMGS
                     DALRKIEEVGEFVPCLHSVGAPLEPGQTDVMWPCNDTKYISHFPETREIWSYGSGYGG
                     NSLLGKKCYALRIASVMARDEGWLAEHMLILKLTSPAGRTHYVAAAFPSACGKTNLAM
                     LEPTIPGWKVETLGDDIAWMRFGPDGRLYAVNPEFGFFGVAPGTGYGTNPNAMRTVEK
                     GNSIFTNVALTDDGDIWWEGMTKEPPAHLTDWKGRDWTPESGEPSSHPNSRFCTPILQ
                     CPIVAPEYDDPAGVPISAIFFGGRRQTTIPLVTEARDWTHGVFMGATLSSETTAAATG
                     AVGVVRRDPFAMLPFLGYHAGDYFGHWLRIGKTHDASKLPRIFYVNWFRKAADGHWLW
                     PGFGENSRVLKWAIERIEGTADASDTPIGYVPTPTSLDVSGLDLSEADIAAALRVEPD
                     EWQAEIPAISEWFEKFGDKLPAVLWTELDGLKARLGTR"
     gene            complement(2739..4913)
                     /locus_tag="VLC50_01880"
     CDS             complement(2739..4913)
                     /locus_tag="VLC50_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011728359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HSF26245.1"
                     /translation="MGRSGPPAATPLGAGLTPERALELFDSQLGSRHLDLAARWLRAQ
                     GKGFYTIGSSGHEGNAAVAAALRPTDPALLHYRSGAFYVERARQVGGVDPLRDVLLGV
                     VAAASEPIAGGRHKVFGRRELAVIPQTSTIASHLPRAVGVAFAIERATRLGVACDWPA
                     DAVTVCSFGDASVNHSTAAGAINAAIQVGFRGLPLPLLLVCEDNGLGISVPTPAGWVE
                     HAYGARPGLRYFVADGCDLVDAVGTAAEAAAWVREHRRPAFLHLRTVRLMAHAGSDVE
                     TAYRRPQAIAADLKRDPLLRTVEILTAAGVLTPDEALARYEAKRARVLAIAREVADLP
                     PLDSAAAVMAPLALPPEDVVRRTATAVARPGRRAALIGTPLPEHEPPLTLAQSINRAL
                     LDVLVTHPGTVVFGEDVAAKGGVYGVTRGLAKRVGTARVFDTLLDEQTILGLALGAGL
                     SGLLPIPEIQYLAYLHNAADQLRGEAATLRFFSQGQYRNPMVVRIAGYGYQKGFGGHF
                     HNDNAIAALRDIPGLVIASPARPDDAAAMLRTCVGAALADGAVCVYLEPIALYHARDL
                     YAEGDGGWLAPYVGPAQWAGEHVPIGRARTYGDGGDLTLVSYANGLFMSLRVAARLEA
                     AGLRCRVVDLRWLAPLPVQDLLREAEATGTVLVVDETRRSGGVAEGVLAALVDAGFAG
                     SMARVTSRDSVIPLGDAALHVLVSEADIERAARRLLGDALGA"
     gene            complement(4990..5646)
                     /locus_tag="VLC50_01885"
     CDS             complement(4990..5646)
                     /locus_tag="VLC50_01885"
                     /inference="COORDINATES: protein motif:HMM:NF013353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036361 - racemase activity, acting on
                     amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HSF26246.1"
                     /translation="MRLLFINPNTTASMTRSIGAQARAVAAGTTEIVTVNPPRGPAAI
                     ETHDDEAAATEAMLGMLQAVDADAFDGIAIACFGDPGLAELRAAVGVPVVGIAESAML
                     LACAMGPRFAIVAALDSAVPMMEELAAGYGLAARCAGVRATGLSVLQVAADPEASRPV
                     IEATCRAAITVDGADSVCLGCAAMSPLAASLTRTLGRPVLDGVQAAVKVLEAITAVRR
                     "
     gene            complement(5661..7058)
                     /gene="hydA"
                     /locus_tag="VLC50_01890"
     CDS             complement(5661..7058)
                     /gene="hydA"
                     /locus_tag="VLC50_01890"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003385640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004157 - dihydropyrimidinase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HSF26247.1"
                     /translation="MRTLLRGGTVVTAADTFRADVLIDGTRITALADSIDAPADRVLE
                     VDGALLLPGLVDSHTHLAMPTMGTVTADDFDTGTAAAAAGGTTTVVDFAMQTDGSLVT
                     GLRAWHERAAGRTHIDYGFHMAITEASPRALAEMAELVDAGVSSFKVFMAFKGSFMAD
                     DDALLRVLRRTRETGGLVQVHAENGDAIELNIADAREAAHTTPRYHAETRPPSTEQEA
                     TSRAVYLARWAARPVFFVHVSCGGAVGEIAAARAAGFPIYGETCTHYLLLTQDELYRE
                     GFEAAKYVCSPPLRTETDQDALWSALRTRTLQVTTTDHCPFNFCGQKELGRDDFSKIP
                     NGLPTIEHRLTLLHQHGVRTGRLAWPELVDLTSTTPARLFGLERKGRIAPGFDADIVV
                     FDPQAHLTISAGSHHMAVDYTPFEGWECQGAPALVLSRGEVVYRDGELVSAPGRGQFV
                     RRTTGPFGPGAITAL"
     gene            complement(7058..8593)
                     /locus_tag="VLC50_01895"
     CDS             complement(7058..8593)
                     /locus_tag="VLC50_01895"
                     /inference="COORDINATES: protein motif:HMM:NF014218.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS1 family nucleobase:cation symporter-1"
                     /protein_id="HSF26248.1"
                     /translation="MNSQSMQSPASSTSVEIPDADPLLINADLMPTPVSQRRWTSWNF
                     AALWMGMIHSAFGFAVIGGMIATGMSAWQALGVVFVANMIQMGLMALTGRVGSRHGIP
                     FAVWARSAFGSFGANIPALLRGVVAVGWFGVQSFLGSTAINVLLGTSLSFWKEWDTVI
                     AGVSANLWASMLVYWAINFLLIRHGMETIRRFESWAGPMIFVVMLPLVIWAFAQMDGL
                     GPVFDHATGYSSTGEFMAKGFLPGVALFISASWATMVLNLPDLTRFARSNRSQVKGLF
                     VGLPLATLVFYGMAAIIVSGTQAATGEVLWNPADVLVAIGNPVVAIVGAIAIAVATMS
                     VNLAANIVSPAYDFTNLLPKLLTFKRAASISILLGFLYMPWKLMENPDTLFSVLNNVG
                     AVIGPATGILIADYYIARGRRLDVAALYQRGSAYWAHKGYNYWSIGVLVLGTGFCIMG
                     QFVGAIGWAYDYASVFGPVLGFVLYLLVLPLARKSPIAEAFAPLGDTGEEYDNLASRT
                     AEA"
     gene            complement(8655..9776)
                     /locus_tag="VLC50_01900"
     CDS             complement(8655..9776)
                     /locus_tag="VLC50_01900"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008838 - diaminopropionate ammonia-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HSF26249.1"
                     /translation="MTKAPRLLANPHAGAAPVGAEVETGPVLAFHRSLPRYRPTPLFA
                     APAIAARLGVAQVWVKDESDRLGMPSFKILGASWATFRALCARVGIDHGHVDGLAGLR
                     AALAGHPLTLVAATDGNHGRAVARMARELGLGAHILVPRDMVAARIAALQAEGARVTI
                     VPGTYDEAVAASARLADETHVVVSDTSWPGYEQVPGWVIDGYATIVAEVHDQLAEAGA
                     APPTVVAAQIGVGAFAAAVVRGFPEAAVVGVEPTKAACVMASLAAGALRQVEGDLDSI
                     MAGLNCGTPSPVAWPDLVAGLRHVVTVDDAAAEEAMRVLAAAGVVSGESGAAGLAGLL
                     TFGARLGLGPNDRVLVVSTEGATDPVAYDRIVAHAHSSS"
     gene            complement(9773..10960)
                     /locus_tag="VLC50_01905"
     CDS             complement(9773..10960)
                     /locus_tag="VLC50_01905"
                     /inference="COORDINATES: protein motif:HMM:NF009555.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HSF26250.1"
                     /translation="MSQLQPTSVDPQAVTDFLLRLIAARSYSTDEAAAAAVLHEELTR
                     LGFAVEVDEIGNVVGTLELGEGPTVLLDCHLDTVVVVDPAEWTRDPAGEVVDARVYGR
                     GTVDMKGPMAACVHGVAALRGLGVGRVVVSGTVAEELVEGPSLVRVARRVQPDFVVIC
                     EATARRVAAGQRGRAEVLVEVAGQSCHSAHPQAGLNAAEVMADVIAALRELKPPVHPT
                     LGEGILVLTDVMSHPYPGLSVVPERCVATYDRRTLVGETDEDVLAPVRAIVDEVTARW
                     GTTGTVSIAVDDYTAYTGTQVQAPNFAPAWLAEEDAPIVTAALDGLRAAGLGATVGHY
                     KFCTNGSGTAGHLGIATIGYGPGEEDQAHTVDESIALDDLHEGARGYTAIVAALLQSG
                     DRR"
     gene            complement(11014..12591)
                     /locus_tag="VLC50_01910"
     CDS             complement(11014..12591)
                     /locus_tag="VLC50_01910"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HSF26251.1"
                     /translation="MDAELVIEGVDVVDDHGARRADVAVSGGVIAAVGPNLPIGSAAE
                     RLDGRGRMLCPGFIDLHGHSALRAFDEPLLEPKIAQGFTTELICPDGLGPAPVTPALA
                     PGRRTYLQALEPSTSAPWDWASMDDFLAALEAAGPASSMVTAVPHSAVREVVMGGDDR
                     RPDAGELVAMQELARRCFDAGARAMSFGLIYAPGLYADTAELVAMAEVAAAYDAPLVP
                     HVRNEARGVLDSIDEFVRVAERTGAPLHVSHVKLVGCPDLLTDLVDLLLRAEDRVRLT
                     FDQYPYGAGSTLLAALLPPYAFAGGPAAILARVADATERTRMVRDMDRGLTGWENLYG
                     ACGPDHIVITQAGGRRSDAVGQTLEQVADRAGTDPAVAVLDLLRDTSLDVAMIDHYST
                     EAVVREIFTLPRTLVGSDGVFNPHPHPRLYGTAARVLGRYAMRERLVPVEEAVARLTS
                     RPARLLGLADRGRVAEGLRADLVLLDPTEFVDRATYDDPHQTPSGVELVLVAGRPVWR
                     DGKATGERPGTVCRSTA"
     gene            12700..13467
                     /locus_tag="VLC50_01915"
     CDS             12700..13467
                     /locus_tag="VLC50_01915"
                     /inference="COORDINATES: protein motif:HMM:NF019322.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HSF26252.1"
                     /translation="MVSTPAEAPPRRIRPRLSDEVRDTIVGELLLSGAVPAGSRLPTE
                     AELCRRYGVSRVTVRAALRSLQEAGYIAIRQGQGSTVLPRPQAIPSGLHRLSSLETFA
                     ADQGSQVSSAELEVSELALDPDEAARLERPVGTRALVIRRVKVYDDDRVAWIVDYVPA
                     GVLPFAVIEREFAGSVLDVLLAHHELDVEFSDCDVNAVALPADVARRLAVRKGVPAIF
                     LDELTRTSNGEVVNWSQAWILPEFFRFSVRRTRQFRH"
     gene            13564..14196
                     /locus_tag="VLC50_01920"
     CDS             13564..14196
                     /locus_tag="VLC50_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase"
                     /protein_id="HSF26253.1"
                     /translation="MQRISYVPLEAMDERMQTEMERCAREGTPRPESSAVRAHAPNAF
                     WAFADSWKSLFHEGVCDHAIKDLCRVYISRSVKCEFCGNQRSIKATTAGLLEQQYDQL
                     LNFESSDLYDERQKAALSYAEAIAWHLDVDDAFWERLHKHFSEPELVELGCCIGLTLG
                     QQSWIRLLGIDHHQYMAGTDASMAPGFRTAEELAASKSSPDYWASKSTTH"
     gene            complement(14264..15364)
                     /gene="ugpC"
                     /locus_tag="VLC50_01925"
     CDS             complement(14264..15364)
                     /gene="ugpC"
                     /locus_tag="VLC50_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012725826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HSF26254.1"
                     /translation="MATVTFDKASRLYPGSESPAVDKLDLQIDDGEFLVLVGPSGCGK
                     STSLRMLAGLEEVTEGRILIGERDVTRLQPKDRDIAMVFQNYALYPHMSVAENMGFAL
                     KIAGVSKEERDRRVMEAATLLDLVPFLDRKPKALSGGQRQRVAMGRAIVREPQVFCMD
                     EPLSNLDAKLRVSTRTQIASLQRRLGTTTVYVTHDQVEALTMGDRVAVLKDGVLQQVG
                     TPREMYDRPTNVFVAGFIGSPAMNLFDFELNEAGAIVSDGEVPLPRETIAACRADGNQ
                     VTLGFRPEDVDVEGDGDGQGIPLVVDVIEELGSDAFIYGHLPEQDVLTAKPVIARIDP
                     RTTIERGHTVHLQVRPHRVHAFSTVTGERLPG"
     gene            complement(15883..17142)
                     /locus_tag="VLC50_01930"
     CDS             complement(15883..17142)
                     /locus_tag="VLC50_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011757900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HSF26255.1"
                     /translation="MAQAQKRPAGTAPAPGRMAVDFEERVDFARLRDYRLGRARAALE
                     RSELGAVLCFDVNNIRYISATMIGEWARDKMTRYTLLTRGAEPVVWDFGSAARHHQLH
                     APWLRTEDARAGMLGMRGSVAPEAGLFEAAAREIKGLLEDAGVADQPVGIDLVELPMI
                     FELQKLGIDVRDGQQVMLDARQIKSQDEIMLLSMSAAMVDGCYQSIVDALKPGIRENE
                     VVALANKRLYELGSDDVEAINAVSGERCSPHPHNFTDRIIRPGDQVYFDIIQSYMGYR
                     TCYYRTFAVGRSTPAQRDAYTQCREWIDAAIDMVKPGVGTDEIARLWPRAEDFGFPSE
                     MAAFGLQFGHGLGLALHERPIISRLNSLEHPFELQEGMVFALETYCPASDGFSAARIE
                     EEVVVTADGPLVLTLFPAEELFVTNPY"
     gene            complement(17162..18109)
                     /locus_tag="VLC50_01935"
     CDS             complement(17162..18109)
                     /locus_tag="VLC50_01935"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007542680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HSF26256.1"
                     /translation="MAEDALPNLRVGWIGTGRMGAPMARRLARAGADVTVWNRTRSKA
                     EPLAADGAKIADSILELAGLDVVFTMVSTSADLAHVLTGEGGLLTHAEVAPKVVVDTS
                     TVSTETSAAMRAAAAERGAGFLASPVSGNGKVVASGKLSLVASGQRETFDLVQPLLAT
                     IARSVTYVGEGEVARLVKIAHNVLLGVVTQSLAEITVLAEKGGVSRAAFLDFINNSVM
                     GSVFTRYKTPAFVNLDFKPTFTPILLRKDFELGLAAARALDVPMPVAALTKELVQSTV
                     SQGYTDRDFAVLLEQAAHNAGLTMTPEDVEVDDGLGGQD"
     gene            18242..19798
                     /locus_tag="VLC50_01940"
     CDS             18242..19798
                     /locus_tag="VLC50_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020509618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HSF26257.1"
                     /translation="MASDTAPSQADVVIIGAGHNALVCAGYLAAAGLQVIVLEAGAVV
                     GGNTATEELTLPGFQHDSCSSAHVLIQSNPVIRDDELGLCARYGLRYERVDPAVVMPL
                     PDGDALVMHRDLDATADELARFSPADATALRALMADWDGGLATAHGRWNDGVPPGQTA
                     ADAAYAALRRRSSREVIESTFTHPVVRSLMMWLAFATFQPPDRPGTGVLPLAITSGRL
                     RHGWATPVGGSAALPQALARLVGDHGGTVLTAARVSRILVEHGRACGVETTDGRRFTA
                     RRAVVSAAHLAQLPEMLEPASCPAQLRAAAQAWRPGLSLFAVHVALRHDIRVHTGAGP
                     RTAVAAALGSGAGVMAQLQAFRRGEVETADPWILVVSPTVVDPDRAPDGGGTLKLLTS
                     APYERADGRDWAQARDEYAAVLLDRVREHADGLDEDNVLAVLPASPVDLAAHNPANLG
                     GSCHGGELVEPDGSVSPGWRSHRSPLPGLYLTGSTTHPGGSVSGRPGRNTARVLLDDL
                     GIGATTVMGA"
     gene            19802..>20397
                     /locus_tag="VLC50_01945"
     CDS             19802..>20397
                     /locus_tag="VLC50_01945"
                     /inference="COORDINATES: protein motif:HMM:NF024376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HSF26258.1"
                     /translation="MATATRVRVGLFSPSIVLAVAEETGGLARRGVEVETVPVTSSPA
                     QFRDLMSGELDLAITNPDNVMLYRFIAANPLGSRHDVRILLGVDRGLGLSVHLAPGLG
                     SLDQLRGGRLGVDVDASGFSFAAYDLLARHGLRRDEDYTVVELGSTPRRLRVLLAGEC
                     EATMLGAGNDLLADDAGLTRAVRLTDEVSPYLATVVAAS"
BASE COUNT         2968 a         7275 c         7340 g         2814 t
ORIGIN      
        1 ttgagcgcga ccgggtccag gaggcgggcc tgcttggtca ggaacgccac cgccgctgct
       61 cgggtggcca catccacggt gccgagcttg tcgatgtctt tcagggtgtc ggcgacgatc
      121 cgggcgtgcg ccagcgtcgc agccccggcc gcgagcaggc cggcggcttc cgggcagccg
      181 tcgagcatcc cggcgacctt gacgtgctca cgggccgtcg ccccgctgat ccgggccacg
      241 tccgccagcc acgacgccgt cgtacgggcc ttcagctgct cacgcacccg acggacctcg
      301 gcgtgggcca gcagagaggc gcggatcgcg accagccggg cctcggctgc ggcgaccgcg
      361 tccaacccgg cgcgggtgtc ctcatcggag agcaggctga cccggtcccg gccgggcccg
      421 gccgcggtgg tcagcccttt ggtggtcagg tgcagcccgg ccagcagcgg atgcccactg
      481 cctacccagt tcgtgctgct cgccccgtcc atgaccccac gctagaggtc acctctgaca
      541 gtcccgcccg acccgaaacc cgttgtggac aaacggcttc cgcgcctcgc ctgtggattc
      601 tctcggccgc cattcgcgca tgatcagtgc aggaaggaga gcgatggttt gcgcatgatc
      661 agtgcagagg tgggagcggg cgggagggca gggatggggc gcagggcaaa gagggcatag
      721 gggtgcagag cgccgcacgc gcggactccg cgtgctgcga atcttggcgg atcgccccaa
      781 tgatctcagc agatgagatt tcctgggtgc cacgacgggg gcgccatagc cgtgcgtccc
      841 gtgcggtgca gcaccgacgc agcgccacgc aaaagccgac gagtgaaggg atctccacgc
      901 atgagtgccg agaccgtcgc cgcgcccacc acccaccaac ccctgatcga ctgggtcgca
      961 gaggtcgccg cgctgaccga acccgaccga gtcgtctggg ccgacgggtc cgacgaggag
     1021 tggcgccggc tcaccgacga gctctgcgag acgggcgtgc tcgtgcggct ggacgagtcg
     1081 cgcaagccca actcgttctg ggcccgcacc gatccctccg acgtggcgcg ggtggagggc
     1141 cgcacgttca tctgctccga gcgtgaggag gacgccggcc cgaccaacaa ctggatggct
     1201 ccggcggaga tgaaggcgac catgaccgag ctgttccgcg gctcgatgca tggccggacg
     1261 atgtacgtca tcccgttctg catgggcccg ctgtcggcgg agcggcccaa gctcggcgtg
     1321 gagatcaccg actcggcgta cgtcgcggcc tcgatgcgaa tcatgacccg gatgggctct
     1381 gacgcgctgc gcaagatcga agaggtcggc gagttcgtcc cgtgcctgca ctcggtgggc
     1441 gcaccgttgg agcccgggca gaccgacgtg atgtggccct gcaacgacac gaaatacatc
     1501 tcgcactttc cggagacccg cgagatctgg tcctacggct ccggctatgg cggcaactcc
     1561 ttgctgggca agaagtgcta cgcgctgcgg atcgcctcgg tgatggcccg cgacgagggc
     1621 tggctggccg agcacatgct catcctcaag ctgacctccc ccgccgggcg cacccactac
     1681 gtggccgcgg ccttcccgag cgcctgcggc aagaccaacc ttgcgatgct cgagccgaca
     1741 atccccggct ggaaggtgga gacgctcggc gacgacatcg cctggatgcg cttcggcccg
     1801 gacgggcgcc tctacgccgt caaccccgag ttcgggttct tcggcgtcgc gcctggcacc
     1861 ggttatggca ccaaccccaa cgcgatgcgc accgtggaga agggcaactc gatcttcacc
     1921 aacgtcgccc tcaccgacga cggcgacatc tggtgggagg gcatgaccaa ggagccgccg
     1981 gcccacctga ccgactggaa gggccgcgac tggacacccg agtccggtga gccgtcgtcg
     2041 caccccaact cccggttctg caccccgatc ctgcagtgcc cgatcgtggc gccggagtac
     2101 gacgacccgg caggggtgcc gatctcggcg atcttcttcg gcggccgccg gcagaccacc
     2161 atcccgttgg tcaccgaggc ccgcgactgg acgcacggcg tgttcatggg ggcgacgctg
     2221 tcgtcggaga ccaccgcggc ggcgaccggc gcggtgggcg tcgtacgccg tgatcccttt
     2281 gccatgctgc cgttcctggg ctaccacgcc ggcgactact tcgggcactg gctgcgcatc
     2341 ggcaagaccc acgacgcgtc caagctgccg cggatcttct acgtcaactg gttccgcaag
     2401 gccgccgacg ggcactggct ctggcccggc ttcggcgaga actcccgcgt gctcaagtgg
     2461 gcgatcgagc ggatcgaggg tacggcggac gcgagcgaca ccccgatcgg ctacgtgccg
     2521 accccgacct cactcgacgt gtccgggctc gacctgagcg aggccgacat cgcggcggcg
     2581 ctgcgcgtgg aaccggacga gtggcaggcc gagatcccgg cgatcagcga gtggttcgag
     2641 aagttcggcg acaagttgcc agccgtgctg tggaccgagc tggacgggct caaggcgcgt
     2701 ctcggcacgc ggtgacggcc gccgggcgac cctgacacct acgcaccgag cgcgtcgccc
     2761 agcagtcgcc gcgcggcgcg ctcgatgtcc gcctcggaga cgagcacgtg caaggcggca
     2821 tcgccgagcg ggatcacgct gtcacggctg gtgacgcgcg ccatggaccc ggcgaacccg
     2881 gcatcgacca gggcggccag gacaccctcg gccaccccgc cggaccgccg ggtctcgtcg
     2941 accaccagca cggtgcccgt cgcctccgcc tcgcgcagca ggtcctgcac cggcaacgga
     3001 gcgagccacc gcaggtcgac gacccggcag cggaggccgg ccgcctcgag ccgggccgcc
     3061 acccgaaggc tcatgaacag cccgttggca tagctcacca gcgtgaggtc gccgccgtcg
     3121 ccataggtgc gcgcccgacc gatcggcacg tgctcgcctg cccactgcgc ggggccgacg
     3181 tagggcgcca gccaccctcc gtcgccctcg gcgtagaggt cgcgggcgtg gtagagcgcg
     3241 atcggctcga ggtagacgca caccgcgccg tcggcgagcg ccgcgccgac gcaggtgcgc
     3301 agcatcgcgg cggcgtcgtc cgggcgcgcc ggtgaggcga tgacgagccc cgggatgtcg
     3361 cgcagcgcgg cgatcgcatt gtcgttgtgg aagtgcccgc cgaagccctt ctggtagcca
     3421 tatccggcaa tccgcacgac catcgggttg cggtactgcc cctgcgagaa gaaccgcagg
     3481 gtggccgcct cgccgcgcag ctggtcggcc gcattgtgca aataggccag atattggatc
     3541 tccggtatcg gcagcagccc cgacagcccg gcgccgaggg cgagcccgag gatcgtctgc
     3601 tcgtcgagca gcgtgtcgaa gacccgcgcg gtccccaccc gcttggccag gccgcgggtg
     3661 acgccataga cacccccctt ggcggcgacg tcctccccga acaccacggt gccgggatgg
     3721 gtgacgagca cgtcgagcag ggcccggttg atcgactgcg ccagcgtgag cgggggctcg
     3781 tgctcgggca acggggtccc gatgagcgcg gcgcgccgcc ccggccgggc aacggcggtc
     3841 gcggtccggc gcacgacgtc ctccggcggg agcgccagcg gcgccatcac cgcggcggcc
     3901 gagtcgagcg ggggcaggtc ggcgacctcc cgcgcgatcg ccagcacccg ggcccgcttg
     3961 gcctcatagc gggccagcgc ctcgtcgggg gtgagcaccc cggcggccgt gaggatctcg
     4021 acggtgcgca gcagcgggtc gcgcttgagg tcggccgcga tggcctgggg gcggcggtag
     4081 gcggtctcga cgtccgagcc ggcgtgcgcc atcagccgga cggtgcgcag gtgcaggaag
     4141 gccgggcgcc ggtgctcgcg cacccaggcg gcggcctcgg ccgccgtgcc caccgcgtcg
     4201 acgaggtcgc agccgtcggc gacgaagtag cgcagcccgg gccgggcgcc gtaggcgtgc
     4261 tccacccagc cggccggggt cggcacgctg atgcccaggc cgttgtcctc gcagaccagc
     4321 agcagcggca gcggcagccc gcggaagccg acctggatcg ccgcgttgat cgcaccggct
     4381 gcggtcgagt ggttgaccga cgcgtcgcca aagctgcaga ccgtcacggc gtcggccggc
     4441 cagtcgcagg ccacaccgag ccgggtggcg cgctcgatcg cgaacgcgac ccccaccgcg
     4501 cgcggcaggt gcgaggcgat ggtcgacgtc tgggggatga cggcgagctc tcggcggccg
     4561 aacaccttgt gacggccccc ggcgatcggc tcgctggcgg cggcgacgac accgagcagt
     4621 acgtcgcgca gcgggtcgac gcccccgacc tggcgggcgc gttcgacgta gaaggcgcct
     4681 gaccggtagt gcagcagggc tggatcggtg ggccgcagcg ccgcggcgac ggccgcgttg
     4741 ccctcgtgtc ccgacgaccc gatcgtgtag aaccccttgc cctgggcccg cagccagcgg
     4801 gcggccaggt cgaggtgacg gctgccgagc tggctgtcga acagctcgag cgcccgctct
     4861 ggtgtcagcc cggccccgag cggcgtggcc gccggtggcc cggatcggcc cagctcggcc
     4921 acggcctcga cgaagcactc atccacctgc tcgacccagc ccgcccgctc gcccacgcgg
     4981 ccgatcctgt caccggcgta ccgcggtgat cgcctcgagg accttgaccg cggcctgcac
     5041 cccgtcgagc accggtcggc cgagggtgcg cgtcagcgat gcggcgagcg ggctcatcgc
     5101 ggcgcagccg aggcagacgc tgtcggcccc gtcaaccgtg atggccgccc ggcacgtggc
     5161 ctcgatgacc gggcggctgg cctcggggtc ggcggccacc tgcagcacgc tgaggcccgt
     5221 cgcgcgcacc ccggcgcagc gcgcggccag cccatagccc gcggcgagct cttccatcat
     5281 cggtacggcg gagtcgaggg ccgcgacgat cgcgaagcgt ggccccatgg cgcaggccag
     5341 cagcatcgcc gactcggcga tgccgaccac cggcacaccc accgccgcgc gcagctccgc
     5401 caggccgggg tcgccgaagc aggcgatcgc gatgccgtcg aaggcgtcgg cgtcaactgc
     5461 ctggagcatc cccagcatcg cctcggtggc ggccgcctcg tcgtcgtggg tctcgatcgc
     5521 ggccgggccg cgcggcgggt tgaccgtgac gatctcggtc gtccccgccg cgaccgcccg
     5581 ggcctgcgcg ccgatgctgc gggtcatcga cgccgtggtg ttggggttga tgaagagcag
     5641 cctcatgaac ggtccggtgg ctagagcgcc gtgatggcac cgggcccgaa cgggccggtc
     5701 gtgcgccgga cgaactgccc ccggcccggc gcgctgacca gctcgccgtc gcggtagacc
     5761 acctcgccgc ggctgaggac gagggccgga gccccctggc actcccagcc ctcgaacggc
     5821 gtgtagtcga ccgccatgtg gtgcgagcct gcggagatcg tcaggtgcgc ctgcgggtcg
     5881 aacaccacga tgtccgcgtc aaagcccggc gcgatccggc ccttgcgctc gagcccgaac
     5941 aaccgcgccg gcgtcgtcga cgtgaggtct accagctcgg gccaggccag ccgcccggtc
     6001 cgcacgccgt gctggtgcag cagggtgagc cggtgctcga tcgtgggcaa cccgttgggg
     6061 atcttggaga agtcgtcgcg accgagctcc ttctggccgc agaagttgaa cgggcagtgg
     6121 tcggtcgtgg tgacctgcag ggtccgggtc cgcagcgccg accacagcgc gtcctggtcg
     6181 gtctcggtcc gcagcggcgg agagcagacg tatttcgccg cctcgaagcc ctcgcggtag
     6241 agctcgtcct gcgtgagcag caggtagtgc gtgcacgtct cgccgtagat cgggaagccg
     6301 gcggcgcgag ccgccgcgat ctcccccacc gccccaccgc acgagacgtg cacgaagaac
     6361 accgggcgcg ccgcccagcg cgcgagatag accgcccgcg acgtggcctc ctgctcggtg
     6421 ctgggcgggc gggtctcggc gtggtagcgc ggggtggtgt gggccgcctc gcgcgcgtcg
     6481 gcgatgttga gctcgatcgc gtcgccgttc tcggcgtgca cctgaacgag tccgccggtc
     6541 tcgcgggtac ggcgcagcac gcgcagcaag gcatcgtcgt cggccatgaa cgagccctta
     6601 aacgccatga acaccttgaa cgagctcacg ccggcgtcga cgagctcggc catctcagcc
     6661 agcgcccggg ggctggcctc ggtgatggcc atgtggaagc cgtagtcgat gtgggtccgg
     6721 ccggcggccc gctcgtgcca ggcacgaagc ccggtcacga gcgagccgtc ggtctgcatc
     6781 gcgaagtcga ccaccgtggt ggtgccgccg gcggcggcag ccgccgtacc ggtgtcgaag
     6841 tcgtccgcgg tgaccgtgcc catggtgggc atcgcgagat gggtgtggct gtcgaccagt
     6901 cctggcagca gcagagcgcc gtccacctcg agcacccggt cggccggggc gtcgatcgag
     6961 tcggccagcg ccgtgatcct cgtcccgtcg atgagaacgt cggctcggaa ggtgtcagca
     7021 gcagtgacga cggtgccgcc acgcaggagt gtgcgcatca ggcctccgcg gtccgagacg
     7081 ccaggttgtc gtactcctcg ccggtgtcgc ccaggggagc gaacgcctcg gcgatcgggc
     7141 tcttgcgggc caatggcagc acgagcaggt agagcacgaa cccgagcacc ggaccgaaga
     7201 ccgacgcgta gtcgtaggcc cagccgatcg caccgacgaa ctgacccatg atgcagaagc
     7261 cggtgccgag gacgagcaca ccgatcgacc agtagttgta gcccttgtgc gcccagtagg
     7321 cgctgccgcg ctggtagagc gcggccacgt cgagccggcg gccgcgggcg atgtagtagt
     7381 cggcgatcag gatgccggtg gccgggccga tgaccgcgcc gacgttgttg aggaccgaga
     7441 acagcgtgtc cgggttctcc atgagcttcc agggcatgta gaggaagccg agcaggatgc
     7501 tgatgctcgc ggcccgcttg aaggtgagca gcttgggcag caggttggtg aagtcgtagg
     7561 ccggcgagac gatgttggcg gccaggttga ccgacatggt ggccacggcg atcgcgatgg
     7621 cgccaacgat ggccacgacc gggttgccga tcgcgacgag cacgtcggcc gggttccaga
     7681 gcacctcacc ggtggcggcc tgggtgcccg acacgatgat ggccgccatg ccgtaaaaga
     7741 ccagggtggc cagtggcagc ccgacgaaca ggcccttgac ctgcgagcgg ttggaccggg
     7801 cgaagcgggt caggtcaggc aggttgagca ccatggtggc ccagctggcc gagatgaaca
     7861 gggcgacacc gggcaggaag cccttggcca tgaactcacc ggtcgaggag tagccggtcg
     7921 cgtggtcgaa caccggcccg agcccgtcca tctgggcgaa ggcccagatc accagcggca
     7981 gcatgacaac gaagatcatc ggcccggccc agctctcgaa ccgccggatc gtctccatgc
     8041 catggcggat cagcaggaag ttgatcgccc agtagacgag catcgaggcc cacaggttgg
     8101 ccgagacccc ggcgatgacg gtgtcccact ccttccagaa cgacaggctg gtgcccagca
     8161 gcacgttgat cgcggtggag cccaggaacg actggacgcc gaaccacccg acggcgacga
     8221 cgccacgcag aagagccgga atgttggccc cgaacgagcc gaacgccgaa cgggcccaga
     8281 ccgcgaacgg gatcccgtgc cgcgagccga cccggccggt cagggccatg agccccatct
     8341 ggatcatgtt ggccacgaag acgacaccca gcgcctgcca ggcggacatc ccggtggcga
     8401 tcatcccccc gatgaccgcg aagccgaacg cgctgtggat catgcccatc cacagggcgg
     8461 cgaagttcca cgaggtccag cgacgctggc tgaccggcgt cggcatgagg tccgcattga
     8521 tgagcagggg atccgcgtcg gggatctcca cgctcgtcga cgaggccggc gactgcattg
     8581 attgcgaatt caccatgtcg atttcctctg gtcggtgcgg gacaggggtt ggaggtgcgg
     8641 tggacggtgc tgcgtcagct cgagctgtgg gcgtgcgcga cgatgcggtc gtaggccact
     8701 gggtcggtgg cgccctcggt ggagacgacc aggacgcggt cgttcgggcc gaggccgagc
     8761 cgggcgccga aggtgagcag gccggccagg ccggcggccc cgctctcccc cgacacgaca
     8821 ccggcagcag ccagcacccg catcgcctcc tcggcggcgg cgtcgtcgac ggtgacgacg
     8881 tggcgcaggc cggccaccag gtcgggccag gccaccggtg agggggtgcc gcagttgagc
     8941 ccggccatga tcgagtcgag gtcgccctcg acctggcgca gggccccggc cgcgaggctg
     9001 gccatcacac aggccgcctt ggtgggttcg accccgacga ccgcggcctc ggggaacccg
     9061 cgcacgacgg ccgccgcgaa ggccccgacc ccgatctggg cggccaccac ggtcggcggt
     9121 gccgcgccgg cctcggccag ctggtcatgc acctcggcca cgatcgtcgc gtagccgtcg
     9181 atgacccagc cgggcacctg ctcatagccg ggccaggacg tgtccgacac gacgacgtgc
     9241 gtctcgtcgg ccagccgggc cgaggcggcc acggcctcgt cgtacgtgcc gggcacgatc
     9301 gtgacccggg cgccctcggc ctgcagcgcc gcgatccgcg ccgccaccat gtcccggggc
     9361 accaggatgt gcgcgccgag cccgagctcg cgggccatcc gggccaccgc ccggccgtgg
     9421 ttgccgtcgg tggcggccac cagggtgagc gggtgtccgg cgagggcggc gcgcagcccg
     9481 gccagcccgt cgacgtggcc gtggtcgatg ccgacccgtg cgcacagcgc ccggaacgtc
     9541 gcccaggacg ctccgaggat cttgaacgac ggcatgccaa gccggtcgga ctcgtccttg
     9601 acccagacct gcgccacccc gagccgggcc gcgatcgcag gggccgcgaa cagcggggtc
     9661 gggcggtagc ggggcaggct gcggtggaac gcgagcaccg gcccggtctc cacctcggcg
     9721 ccgaccggtg ccgccccggc gtgcgggttg gccagcagcc ggggcgcctt ggtcatcggc
     9781 ggtctcccga ctgcagcagc gccgcgacga tcgcggtgta gccgcgggca ccctcgtgca
     9841 ggtcgtcgag cgcgatcgac tcgtccacgg tgtgcgcctg gtcctcctcg ccggggccgt
     9901 agccgatggt ggcgatgccc aggtgcccgg ccgtgccgga cccgttggtg cagaacttgt
     9961 agtggcccac tgtcgcgccg agcccggccg cgcgcagccc gtccaaggcc gcggtgacga
    10021 tcggcgcgtc ctcctcggcg agccaggccg gggcgaagtt gggcgcctgg acctgggtgc
    10081 cggtgtaggc ggtgtagtcg tcgacggcga tgctcaccgt gccggtggtg ccccagcggg
    10141 cggtgacctc gtcgacgatg gcccgcaccg gggcgagcac gtcctcgtcg gtctcaccga
    10201 cgagcgtgcg ccggtcgtag gtcgccacgc accgctcggg caccaccgac aggcccgggt
    10261 aggggtgcga catcacgtcg gtgagcacga ggatgccctc gccgagggtg gggtgcaccg
    10321 gcggcttgag ctcgcgcagc gcggcgatga cgtcggccat cacctcggcg gcgttcaggc
    10381 cggcctgcgg gtgcgccgag tggcaggact ggccggccac ctcgacgagc acctcggccc
    10441 ggccgcgctg cccggcggcg acccggcggg cggtggcctc gcagatcacc acgaagtccg
    10501 gctggacccg ccgcgccacc cgcaccagcg acgggccctc gacgagctcc tcggccacgg
    10561 tgccggacac cacgacccgg ccgaccccca ggccacgcag cgcggcgacg ccgtgcacgc
    10621 aggccgccat cggccccttc atgtcgacgg tgccgcggcc gtagacccgc gcgtcgacga
    10681 cctcgccggc cgggtcgcgg gtccactcgg ccgggtcgac gacgacgacg gtgtcgaggt
    10741 ggcagtcgag cagcacggtc gggccctccc ccagctcgag ggtgccgaca acgttgccga
    10801 tctcgtcgac ctcgacggcg aacccgagcc gcgtcagctc ttcgtgcagc accgccgcgg
    10861 cggctgcctc gtcggtcgag tagctgcgag cggcgatgag ccggagcagg aagtcggtga
    10921 ccgcctgcgg gtcgacgctg gtgggctgga gctgggacat cggtctctct cttcgttggg
    10981 gcacggtgtg cggtgctggt ggtgcggggg ctgctaggcg gtgctgcggc agacggtgcc
    11041 cggccgctcg ccggtcgcct tgccgtcgcg ccagaccgga cggccggcga cgagcacgag
    11101 ctcgacaccg gagggtgtct ggtgcgggtc gtcgtaggtg gcccggtcga cgaactcggt
    11161 cgggtcgagc aggaccaggt cggcccgcag cccctcggcc acccggccgc ggtcggcgag
    11221 cccgagcagc cgggccggtc ggctggtgag gcgggccacg gcctcctcga ccggcaccag
    11281 ccgctcgcgc atcgcatagc ggcccaggac cctggccgcg gttccgtaga gccgggggtg
    11341 cgggtgcggg ttgaacaccc cgtcggagcc gaccagcgtc cgcggcaatg tgaagatctc
    11401 gcggaccacg gcctcggtgg agtagtggtc gatcatcgcc acgtccaggc tcgtgtcgcg
    11461 gagcaggtcg agcacagcca ctgccgggtc ggtgccggcc cggtcggcca cctgctcaag
    11521 cgtctgcccc accgcgtcgg accggcgccc gccagcctgc gtgatcacga tgtggtccgg
    11581 cccgcaggcg ccgtacaggt tctcccagcc ggtcagcccg cgatccatgt cgcgcaccat
    11641 ccgggtccgc tcggtcgcgt ccgcgacccg ggccaggatc gcggccggcc ctccagcgaa
    11701 ggcgtacggc ggcagcagcg cggccagcag cgtgctgccg gcgccgtacg ggtattggtc
    11761 gaaggtcagc cggacccggt cctcggcgcg cagcagcagg tcgaccaggt cggtgagcag
    11821 gtccgggcag ccgaccagct tgacgtgcga gacgtgcagc ggtgccccgg tgcgctcggc
    11881 gacgcggacg aactcgtcga tcgagtcgag caccccgcgg gcctcgttgc gcacgtgcgg
    11941 caccagcggg gcgtcgtagg ccgcggcgac ctcggccatc gcgaccagct cggcggtgtc
    12001 ggcgtacaga cccggggcgt agatgagccc gaacgacatc gcccgggcgc cggcgtcgaa
    12061 gcacctgcgg gccagctcct gcatcgcgac gagctcgccc gcgtcgggcc ggcggtcgtc
    12121 accgcccatc acgacctcgc gcaccgcgct gtgaggcacc gccgtgacca tgctcgacgc
    12181 cgggccggct gcctcgagcg cggccaggaa gtcgtccatg ctggcccagt cccacggcgc
    12241 cgaggtgctc ggctcgaggg cctgcaggta ggtgcgccga cccggcgcca gtgcgggggt
    12301 gaccggggcg ggccctagcc cgtcggggca gatcagctcg gtggtgaagc cttgggcgat
    12361 cttgggctcg agcagcggct cgtcgaacgc gcgcagcgcg ctgtgcccgt gcaggtcgat
    12421 gaaaccgggg cagagcatcc ggccgcgccc gtcgaggcgc tcagccgccg acccgatcgg
    12481 caggttgggg ccgaccgcgg cgatgacccc gcccgagacg gccacgtcgg cacggcgtgc
    12541 accgtggtcg tcgacgacgt cgactccctc gatgaccagc tcggcatcca tgtcctgacc
    12601 cttcccagac tgtgcggtgc ggcaccagcg tggagatttg taggacaact gtcaagaggt
    12661 tgttagccaa atatttgtca gggatgcgag gatgtcgtca tggtcagcac tccggccgag
    12721 gccccgccac gacgcatccg tccccggctc agcgacgagg tccgcgacac gatcgtcggc
    12781 gagctgctgc tcagcggcgc cgtaccggct ggttcgcggc tgcccaccga ggccgagctg
    12841 tgccggcggt acggcgtctc ccgggtcacc gtccgcgccg ccctgcgcag cctgcaggag
    12901 gccggctaca tcgcgatccg ccagggccag ggctcgacgg tgctcccccg cccccaggcc
    12961 atcccgtccg ggctgcaccg gttgagctcg ctcgagacct tcgccgccga ccaggggtcc
    13021 caggtgtcca gcgcggagct cgaggtcagc gagcttgccc tcgaccccga cgaggcggcc
    13081 cggctcgagc gcccggtcgg cacccgtgcg ctggtgatcc ggcgggtcaa ggtctacgac
    13141 gacgaccggg tggcctggat cgtcgactac gtgcccgccg gcgtgctgcc gttcgcggtc
    13201 atcgagcggg agttcgccgg ctcggtgctc gacgtcctgc tcgcccacca tgagctcgat
    13261 gtcgagttca gcgactgcga cgtcaacgcg gtggctctgc ccgcggatgt cgcacggcgg
    13321 ctcgcggtgc gcaagggcgt gccggcgatc ttcctcgacg agctgacccg tacgagcaac
    13381 ggagaggtcg tcaactggag ccaggcgtgg atcctgcccg agttcttccg cttcagcgtc
    13441 cggcgtaccc gccagttccg gcactgaacg gggcgggcgg cagcgacgtg tcccttgtcg
    13501 ggcagccgcc atcgcggcta cattgaggcc agcaggtgca cacgtttgca aaggagaccc
    13561 ggggtgcaac ggatcagcta cgtcccgctt gaggcgatgg acgaacggat gcagaccgag
    13621 atggagcgct gcgcccgcga gggcacgccc cggcccgaga gctcggcggt gcgtgcgcac
    13681 gcgcccaacg cgttctgggc cttcgccgac tcgtggaagt cgctgttcca cgagggcgtc
    13741 tgcgaccacg ccatcaagga cctgtgccgc gtctacatct cgcgctcggt caagtgcgag
    13801 ttctgcggca accagcgctc gatcaaggcg accacggccg gcctgctcga gcagcagtac
    13861 gaccagctgc tcaacttcga gtcctctgac ctctacgacg agcggcagaa ggccgccctg
    13921 tcctacgccg aggcgatcgc ctggcacctg gacgtcgacg acgcgttctg ggagcggctg
    13981 cacaagcact tctccgagcc cgagctcgtc gagctgggct gctgcatcgg gctgaccttg
    14041 ggccagcaga gctggatccg gctgctcggc atcgaccacc accagtacat ggccggcacc
    14101 gacgcctcga tggcgcccgg gttccgcacc gccgaggagc tggccgcgag caagtcctcc
    14161 cccgactact gggcctccaa gagcaccacg cactgaccag cctcacgcga agggctcggc
    14221 gaccacgcgg tcgccgagcc ctttcgtcgt accgaggggg cggtcagccg ggcagccgct
    14281 caccggtgac ggtcgagaac gcgtgcacgc gatgcggtcg cacctgcagg tgcacggtgt
    14341 gaccgcgctc gatcgtcgta cgcgggtcga tgcgcgcgat gaccggcttg gcggtcagca
    14401 cgtcctgctc gggcaggtgc ccatagatga aggcgtccga gccgagctcc tcgatcacgt
    14461 cgaccaccag cgggatgccc tggccgtcgc cgtcgccttc gacgtcgacg tcctcgggcc
    14521 ggaacccgag cgtgacctgg ttgccgtcgg cgcggcaggc ggcgatggtc tcgcggggca
    14581 gcggcacctc gccgtcggag acgatcgcgc ccgcctcgtt gagctcgaag tcgaagaggt
    14641 tcatcgcggg tgagccgatg aaccccgcga cgaacacgtt ggtcggtcgg tcgtacatct
    14701 cgcgcggggt gcccacctgc tgcagcacgc cgtccttgag caccgccacc cgatcgccca
    14761 tcgtcaacgc ctcgacctgg tcgtgggtga cgtagacggt cgtggtgccc aaccgccgct
    14821 gcagcgaggc gatctgggtg cgggtcgaga cgcgcagctt ggcgtcgagg ttggacagcg
    14881 gctcgtccat gcagaacacc tgcggctccc gcacgatcgc ccggcccatc gccacccgct
    14941 ggcgctggcc accggagagc gccttgggct tgcggtcgag gaacggcacc aggtcgagca
    15001 gggtggccgc ctccatcacc cggcggtcgc gctcctcctt ggagacaccg gcgatcttga
    15061 gggcgaagcc catgttctcg gccacgctca tgtgcgggta gagcgcgtag ttctggaaca
    15121 ccatcgcgat gtcgcggtcc ttgggctgca agcgggtgac gtcgcgctcc ccgatgagga
    15181 tgcgaccctc ggtgacctcc tcgaggccgg cgagcatgcg cagcgaggtg gacttgccgc
    15241 atccggaggg gccgaccagg acgaggaact cgccgtcgtc gatctgcagg tcgagcttgt
    15301 cgaccgccgg ggactccgag ccggggtaga gccggctggc cttgtcaaac gtcacggtag
    15361 ccacactcaa cacatccctt caccggcagg aacgtgccgg acgatccgag tgacgggcgg
    15421 gctgatgccg ccttcacggt aaccgcggga cggccttggc agggttcaca cggacaagct
    15481 ggcatctccc acgatgcggc ggtaggtcac cgggtcggcc gcgccttcgg tggagacgac
    15541 gagcacgcga tcgtgccggt ggagcgggat ctcggatcgg caggcaagca ggccggccgg
    15601 cccggcggcc ccgctctcgc cggacccgaa ccagcggggt cgggcggtag ccaggcaggc
    15661 cgtggtggaa tgcgagcacc tgggtgggtg cgtcggcgga cggcaccggg cgggcaggtt
    15721 cgacgtacgg attggtcacg agccgggcct gcgtcacgtc gcgaccgtag cgatacccct
    15781 gcgaccccgt aaccaagtga gctggcactc cacgaggcgt gcctgccgcg tggagtgcca
    15841 gctcacagga agaacctggt cgtttccccc gaggggggtc ggtcagtagg ggttggtgac
    15901 gaagagctcc tcggccggga acagcgtgag gacgagcggg ccatcggcgg tgacgacgac
    15961 ctcctcctcg atgcgggccg cggagaaccc gtcgctggcc gggcagtagg tctccagcgc
    16021 gaagaccatg ccctcctgca gctcgaaggg gtgctcgagc gagttgagcc ggctgatgat
    16081 cgggcgctcg tgcagtgcca ggccgaggcc gtggccgaac tgcagcccga acgcggccat
    16141 ctcggagggg aacccgaagt cctcggcccg cggccagagc cgggcgatct cgtcggtgcc
    16201 gacccccggc ttgaccatgt cgatggcggc gtcgatccac tcgcggcact gcgtgtaggc
    16261 gtcgcgctgg gccggggtgg accggccgac cgcgaacgtg cggtagtagc aggtccggta
    16321 gcccatgtag gactggatga tgtcgaagta gacctggtcg cccgggcgga tgatccggtc
    16381 ggtgaagttg tgcgggtgcg ggctgcaccg ctcgccggac accgcgttga tcgcctcgac
    16441 gtcgtcggag ccgagctcgt agaggcgctt gttggccagc gcgacgacct cgttctcccg
    16501 gatgcccggc ttgagcgcgt cgacgatcga ctggtagcag ccgtcgacca tggccgccga
    16561 catcgacagc agcatgatct cgtcctgcga cttgatctgg cgggcgtcga gcatcacctg
    16621 ctgcccgtcg cggacgtcga tgccgagctt ttgcagctcg aagatcatcg gcagctcgac
    16681 caggtcgatg ccgaccggct ggtcggccac gccggcgtcc tcgagcagcc ccttgatctc
    16741 gcgggcggcc gcctcgaaca gcccggcctc cggcgccacc gagccgcgca tgccgagcat
    16801 cccggcccgg gcgtcctcgg tgcgcagcca cggcgcgtgc agctggtggt ggcgggccgc
    16861 cgagccgaag tcccagacga ccggctcggc accgcgggtc agcagcgtgt agcgggtcat
    16921 cttgtcccgc gcccactcac cgatcatggt ggcggagatg tagcggatgt tgttgacgtc
    16981 gaagcagagc accgcgccca gctcggagcg ctcgagcgcc gcccgggcgc gccccagccg
    17041 gtagtcacga agccgcgcga agtcgacgcg ctcctcgaag tcgacggcca tccgccccgg
    17101 agccggagcg gtgccggcgg gacgcttctg cgcttgtgcc atgtctgtca tctccgcagc
    17161 ctcagtcttg accgccgagg ccatcgtcga cctcgacgtc ctccggggtc attgtgagcc
    17221 ccgcattgtg cgcggcctgc tccagcagca ccgcgaagtc acgatcggta tagccctggc
    17281 tgaccgtcga ctgcacaagc tccttggtca gcgccgccac cggcatcggt acgtcgagcg
    17341 cacgggcggc cgccaggccg agctcgaagt ccttgcgcag caggatcggg gtgaaggtcg
    17401 gcttgaagtc gaggttgacg aacgccgggg tcttgtagcg ggtgaacacc gagcccatca
    17461 cgctgttgtt gatgaagtcg aggaaggccg cacggctgac cccgcccttc tcggccagca
    17521 cggtgatctc ggccagcgac tgggtcacca cgccgagcag cacgttgtga gcgatcttga
    17581 ccaggcgtgc cacctcgccc tcgccgacgt aggtcaccga ccgggcgatc gtcgccagca
    17641 gcggctgcac aagatcgaac gtctcccgct ggcccgaggc gaccaggctg agcttgccgg
    17701 aggcgacgac cttgccgttg ccgctgaccg gcgaggcgag gaagcccgcg ccgcgctcgg
    17761 cggcagccgc ccgcatcgcg gccgaggtct cggtcgagac cgtcgaggtg tcgacgacca
    17821 ccttgggcgc cacctcggcg tgggtgagca ggccgccctc gccggtgagc acatgggcca
    17881 ggtcggccga ggtcgagacc atcgtgaaca cgacgtcgag cccggccagc tcgaggatgg
    17941 agtcggcgat cttcgcccca tcggcggcca gcggctcggc cttgctccgg gtgcggttcc
    18001 agaccgtgac atcggccccg gcccgggcca ggcggcgcgc catcggggcg cccatccgac
    18061 cggtgccgat ccaacccacc cgcaggttgg gaagcgcgtc ttccgccatg agcaaccatc
    18121 ctctgcaatc gtttgccgaa acctagcgac cagcgggtcg agttgtcaag gattcggcgc
    18181 atgccgctcc cgtgagcggc tgatgattgc aaacgaatgc aagcccggcg cacaatgccg
    18241 catggcctcc gataccgcgc ccagccaggc cgacgtcgtc atcatcggcg ccgggcacaa
    18301 cgccctcgtc tgcgccggct acctcgccgc ggcgggcctg caggtcatcg tgctcgaggc
    18361 cggcgccgtg gtcggcggca acaccgccac cgaagagctg accctgcccg gcttccagca
    18421 cgactcgtgc tccagcgccc acgttctcat ccagtccaac ccggtgatcc gcgacgacga
    18481 gctcgggctg tgcgcgcgct acggcctgcg ctacgagcgc gtcgacccgg cggtggtcat
    18541 gccgcttccc gacggcgacg cgctggtcat gcaccgcgac ctggacgcca ccgccgacga
    18601 gctggcgcgc ttctctcccg ccgacgcgac cgcgctgcgc gccctgatgg ccgactggga
    18661 cggcgggctg gccaccgcgc acggccggtg gaacgacggc gtgccgccgg ggcagaccgc
    18721 ggccgacgcg gcctacgccg cgctgcgccg acgcagctcc cgtgaggtca tcgagtcgac
    18781 gttcacccac cccgtcgtac ggtcgttgat gatgtggctg gcctttgcca cgttccagcc
    18841 acccgaccgg cccggcaccg gcgtgctgcc gctggccatc acctccggcc ggctgcgtca
    18901 cggctgggcg accccggtcg gcggctctgc cgcgttgccg caggcgctgg cccggctggt
    18961 cggggatcac ggcggcacgg tgctcaccgc ggcgcgggtg agccgcatcc tcgtcgagca
    19021 cggccgggcc tgcggcgtcg agacgaccga cgggcgccgg ttcacggccc gccgcgcggt
    19081 cgtgtcggcg gcacacctgg cccagctgcc ggagatgctc gagccggcgt cctgcccggc
    19141 gcagctgcgc gcggcggccc aggcgtggcg gccggggctg agcctgttcg cggtccacgt
    19201 cgcgctgcgc cacgacatcc gggtgcacac cggggccggc ccgcgcacgg cggtggccgc
    19261 cgccctcggc tcgggcgccg gggtgatggc ccagctgcag gcgttccgtc gcggcgaggt
    19321 cgagacggcc gacccgtgga tcctggtcgt cagcccgacc gtggtcgacc ccgaccgggc
    19381 ccccgacggg ggcggcaccc tcaagctgct gacctccgcc ccctacgaac gggccgacgg
    19441 gcgcgactgg gcgcaggccc gagacgagta cgccgcggtg ctgctcgacc gcgtccgcga
    19501 gcacgcagac ggcctcgacg aggacaacgt gctcgccgtc ctgccggcct caccggtcga
    19561 cctggcggcc cacaacccgg ccaacctcgg ggggtcctgc cacggtggtg agctcgtcga
    19621 gccggacggg tcggtcagcc cgggctggcg ctcgcaccgc tcgccgctgc ccggcctcta
    19681 cctgaccgga tcgaccaccc atccgggtgg ctcggtctcg ggacgccccg gccgcaacac
    19741 cgcccgggtg ctgctcgacg acctcggcat cggtgccacg acagtgatgg gagcctgacg
    19801 catggccacg gcaacacggg tgcgggtcgg gctgttcagc ccctcgatcg tcctggcggt
    19861 cgccgaggag accggcgggc tggccaggcg cggcgtcgag gtcgagaccg tgccggtcac
    19921 ctcgtcgccg gcccagtttc gcgacctgat gtccggcgag ctcgacctgg ccatcaccaa
    19981 ccccgacaac gtgatgctct accggttcat cgcggccaat ccgctcggct cccgtcacga
    20041 cgtgcggatc ctgctcggcg tggaccgcgg gctggggctc tcggtgcacc tcgcccccgg
    20101 tctgggcagc ctcgaccagc tgcgcggcgg ccggctcggg gtcgacgtcg acgcctcggg
    20161 cttctcgttc gccgcctacg acctgctggc gcgccacggc ctgcgccgcg acgaggacta
    20221 caccgtcgtc gagctgggct cgaccccgcg ccggctgcgg gtgctgctcg ccggtgagtg
    20281 cgaggccacc atgctcggcg ccggcaacga cctactggcc gacgacgccg gcctgacccg
    20341 cgcggtccgg ctcaccgacg aggtctcgcc ctatctcgcc accgtggtcg ccgcctc
//
LOCUS       DATIBB010000054         4605 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_553096, whole genome shotgun sequence.
ACCESSION   DATIBB010000054 DATIBB010000000
VERSION     DATIBB010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 4605)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4605)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4605
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_553096"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..319
                     /locus_tag="VLC50_01950"
     CDS             <1..319
                     /locus_tag="VLC50_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012087349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HSF26259.1"
                     /translation="RLSEAPDRALRMRELAEAASSSRSRLSHAVARLEERGWVRRTPC
                     PTDRRGQVAELTGAGFAALAAAAPGHVAQVRRSVIDRLSERQLRQLERISRAILANRG
                     STL"
     gene            413..1039
                     /locus_tag="VLC50_01955"
     CDS             413..1039
                     /locus_tag="VLC50_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004000152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HSF26260.1"
                     /translation="MPTLRVDSIGDYIRDQRRNARISLRQLAILAGVSNPYLSQIERG
                     LRHPSAEILQQIAKGLRISAESLYVRAGILEERSTSPEVTAAIHGDHGLTERQKRVLV
                     DIYETFRRENAAVAEATATVAAAASTAADLVDSVGAGLSGSPAALKGSHNGTTRRESA
                     RAKGTTSRVPARSAPPSSASAGRRRQKAEASSTATAAPTSARRSHPKT"
     gene            1062..1571
                     /locus_tag="VLC50_01960"
     CDS             1062..1571
                     /locus_tag="VLC50_01960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF26261.1"
                     /translation="MASKTRYPSLTAELRKALADSTPLYAVAGVGDLAAEKLRTVPAR
                     VLELRERIEPRAVVDDVQARVVALRQELPGRASAAATDLTVRANDAYERLAVRGRVLV
                     GRIGRQRATDKTREQAADTADATRPAATTAHTSARRTSAATKGAATSARKTADSAAEA
                     VQDGTDKLG"
     gene            1675..1983
                     /locus_tag="VLC50_01965"
     CDS             1675..1983
                     /locus_tag="VLC50_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018541425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2516 family protein"
                     /protein_id="HSF26262.1"
                     /translation="MTITAAVASLLWIGLGVVKVVALVDALRRREGSFVAASKQSKKL
                     WLLFLGLGLAFHVITGVLSIVNLAGTIAAFVYLVDVRRGLDAVEGRGRGSSSGPYGPW
                     "
     gene            2041..2781
                     /locus_tag="VLC50_01970"
     CDS             2041..2781
                     /locus_tag="VLC50_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013673576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein"
                     /protein_id="HSF26263.1"
                     /translation="MPAHNADRHGRTDRLELTDATVREWDATVLAASPEAIVLDRSAF
                     YPGGGGQPPDRGVLLWGGVQTRIVGVRRGEDLELIPAEGDPIPPEGTSVRGAVEDDRR
                     TALMRTHSSLHLLSGVVFRDFGALVTGGNMEPLEGRLDFNLGTLPEGFKDAVAAACAK
                     EVAADRAIAVYSLPREQAFEIPDIIRTATNLLPPELEVVRIVDIAGLDVQADGGTHVA
                     STASIGQMEIVKVENKGKGFRRIRVRLG"
     gene            complement(2787..3317)
                     /locus_tag="VLC50_01975"
     CDS             complement(2787..3317)
                     /locus_tag="VLC50_01975"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HSF26264.1"
                     /translation="MSEHPASPATPVLETERLVVRDWLDDEADRMFDMYSRLEVVRFL
                     GAVPTPMASIDEAREAIRRARESNAVAAPLGWWAVVVKAAGHVAGTIGLVRLETGEIE
                     VIWHLHPDSMGNGFATEAARAVLSRGHANGIDEVVALVDVANGPSRNVALRLGLEHRG
                     GAQHRGKVFEVFVSRP"
     gene            3335..3919
                     /locus_tag="VLC50_01980"
     CDS             3335..3919
                     /locus_tag="VLC50_01980"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007385797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HSF26265.1"
                     /translation="MTRMPGPASGRQGEVTVTCVEGDITVQQVDVVVNAANSTLLGGG
                     GVDGAIHRAGGPAILTECRLLRASELPEGLPPGQAVATTAGELPARWVVHTVGPNWRA
                     GQRDRRLLVSCYRESLRVADELGAVSVAFPAISAGVYGWPVLDAAHAAVEGVLAAAPT
                     LDAVRDVRFVLFGPTTYAAFAAAVADLAPPSNPH"
     gene            complement(3969..>4605)
                     /locus_tag="VLC50_01985"
     CDS             complement(3969..>4605)
                     /locus_tag="VLC50_01985"
                     /inference="COORDINATES: protein motif:HMM:NF014741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HSF26266.1"
                     /translation="PEPGSEGDDDPCRDRRTRAQRRLDAFARLARHALAHDPDLPGDQ
                     GGRRPVLSITCDYDAVTHQLTNGVLDTGVKIPNHELRRLACGADILPAVMGGASTVLD
                     LGRTRRLFSTNQRRALELRDRGCTFPGCHQPARACEAHHQDHWEDGGPTDLCNGALLC
                     DYHHDQAHRQGWRSRLARANGYVEWQPPRVLDPAQRWRQHHRHQLDQIRRR"
BASE COUNT          620 a         1642 c         1649 g          694 t
ORIGIN      
        1 ccggctgtcc gaggcccccg accgggcgct gcggatgcgc gagctggccg aggccgcgtc
       61 gtcgagccgc agccggctct cgcacgcggt cgcccggctc gaggagcgcg gctgggtgcg
      121 tcgtacccct tgtcccactg accggcgcgg gcaggtcgcc gagctgaccg gggcggggtt
      181 tgctgcgctc gccgccgcgg cgcctgggca cgtcgcgcag gtgcgtcgca gcgtcatcga
      241 ccggctgagc gagcgtcagc tgcgccagct cgagcggatc agccgagcca tcctcgccaa
      301 ccggggctcg acgctctgag cagcgtgatg acgccgtgac gcacggcgac gtgacctgcg
      361 ccatgcttgc gccgacttgc attttgcttg cagcagttag caccatgggg tcgtgcctac
      421 gctacgagtc gactcgatcg gtgactacat ccgcgaccag cgccgcaatg cacggatctc
      481 cctgcggcag ctcgccattc tcgccggggt ctccaatccc tacctgagcc agatcgagcg
      541 cggcctgcgt catcccagtg ccgagatcct ccagcagatc gccaagggcc tgcgcatctc
      601 ggccgagagc ctctacgtgc gggccggcat cctggaggag cgctcgacca gcccggaggt
      661 caccgccgcg atccatggtg accacgggct caccgagcgg cagaagcgcg tgctcgtcga
      721 catctacgag acctttcgtc gggagaacgc ggcggtggcc gaggcgaccg ccacggtcgc
      781 ggcggcggcg tcgactgccg ccgacctggt cgactcggtg ggcgccggcc tgtccggctc
      841 acccgccgct ctcaagggct cgcacaacgg cacgacccgg cgagagtcgg cgcgggccaa
      901 gggcactacc tcccgcgtac cagcgcgctc ggccccaccc agcagcgcat ccgccggccg
      961 gcgccggcag aaggccgagg cgtcgtccac ggcgactgcc gcgccgacct cggcacggcg
     1021 ctcgcacccc aagacctgac ccccgaacct acggaggaac catggccagc aagacccgtt
     1081 acccgtcgct gaccgccgag ctgcgcaagg cgctggcgga cagcacaccg ctctacgccg
     1141 ttgccggtgt cggcgacctg gccgcggaga agctgcgcac ggtgcccgcc cgagtgctcg
     1201 agctgcgcga gcggatcgag ccgcgagccg tcgtcgacga cgtgcaggcg cgcgtcgtcg
     1261 cgctgcggca ggagctgccg ggtcgggcct cggccgcggc caccgacctg accgtgcgag
     1321 ccaacgacgc ctacgagagg ctcgccgtca ggggcagggt gctcgtcggc cggatcggtc
     1381 gccagcgcgc cacagacaag acccgtgagc aggccgctga cacggccgac gccacccggc
     1441 cggcggccac gaccgcgcac acatccgcgc ggcgcaccag cgcggccacc aagggtgcgg
     1501 ccacctcggc acgcaagacc gccgacagtg ccgccgaggc cgtccaggac ggcaccgaca
     1561 agctgggctg agccggctcg gggcgcgccg acgagctcca tcgcgtcggc acgccaccgg
     1621 agattgctcg cgtaggctcg agccgtgatg gatttcgggt ccagcctctc cttcgtgacg
     1681 atcaccgctg cggtggcatc gctgctgtgg atcgggctcg gcgtcgtcaa ggtcgtcgcg
     1741 ctcgtcgacg cccttcgccg tcgggagggg tcgttcgtcg cggccagcaa gcagtcgaag
     1801 aagctctggt tgctcttcct ggggctgggg ctggcgttcc acgtgatcac cggggtgctc
     1861 agcatcgtca acctggccgg cactatcgcc gcgttcgtct acctggtcga cgtgcggcgc
     1921 ggcctggacg ccgtggaggg acgcgggcgc ggctcgtcca gcggccccta cggaccctgg
     1981 tgatcagtct gcgctgacgg gccaggtcaa ggtccacgac caaggtcgta gtgtgccgtc
     2041 atgcccgcac acaatgccga tcggcacgga cgcaccgacc gcctcgagct caccgacgcc
     2101 accgtgcgcg agtgggatgc cacggtgctg gccgcctcac ccgaggcgat cgtgctcgac
     2161 cgctcggcgt tctatcccgg cggcggtggc cagccacccg accgtggcgt gctgctgtgg
     2221 ggcggggtgc agacccggat cgtcggcgta cgccgcggcg aggatctcga gctgatcccc
     2281 gcggagggtg acccgatccc gcccgagggc acgtccgtgc gtggcgcggt cgaggacgat
     2341 cgtcgtacgg cgctgatgcg cacccactcc agcctgcacc tgctctccgg tgtggtcttc
     2401 cgcgacttcg gcgcgctggt taccggcggc aacatggagc cgctcgaggg ccggctcgac
     2461 ttcaacctgg gcacgctgcc ggaggggttc aaggacgcgg tggccgcggc gtgcgcgaag
     2521 gaggtggccg ccgaccgggc gatcgccgtt tactcgctgc cacgcgagca ggccttcgag
     2581 atccccgaca tcatccggac ggcgaccaac ctgctgccgc ccgagctcga ggtggtgcgc
     2641 atcgtcgaca tcgccgggct cgacgtgcag gccgacggcg gcactcacgt cgcctcgacc
     2701 gcgtcgatcg gtcagatgga gatcgtcaag gttgagaaca agggcaaggg ctttcgccgg
     2761 atccgggtcc gtctgggctg accggctcac ggcctgctga cgaacacctc gaacaccttg
     2821 ccgcgatgct gggcgccgcc gcgatgctcc agacccagcc gtaaggccac gttgcgcgac
     2881 gggccattgg ccacgtcgac cagggcgacc acctcgtcga tcccgttcgc gtggccccgg
     2941 gatagcaccg cccgagcggc ctcggtcgcg aagccgttgc ccatgctgtc gggatgcagg
     3001 tgccagatca cctcgatctc gccggtctcc agccggacga ggccgatcgt cccggcgacg
     3061 tgcccggccg ccttcacgac gaccgcccac cagccgagcg gggcggcgac ggcgttgctc
     3121 tctctcgcgc gccggatcgc ctcgcgcgcc tcgtcgatcg aggccatcgg tgtgggcacc
     3181 gcgccgagga agcgcaccac ctcgagccgc gagtacatgt cgaacatccg gtcggcctcg
     3241 tcgtcgagcc agtcgcgcac gacgagtcgc tcggtctcga gcacgggcgt cgcagggctc
     3301 gccggatgct cgctcattcg ggcaggctaa ccgcatgacg cgtatgcccg gtccggcttc
     3361 gggacgccag ggtgaggtca ccgtcacctg cgtcgagggg gacatcacag tgcagcaggt
     3421 cgatgtggtg gtcaatgccg ccaattcgac gttgctcggt ggcggtggcg tcgacggggc
     3481 gatccaccgg gccggggggc cggccatcct caccgagtgc cggctgctgc gcgccagcga
     3541 gctgcccgag ggcctgccgc cgggtcaggc ggtggccacc accgccgggg agctgcctgc
     3601 tcgctgggtc gtgcacaccg tcggccccaa ctggcgtgcc gggcagcgcg accgccgact
     3661 gctcgtctcc tgctaccgcg agtcgcttcg ggttgccgat gagctgggcg cggtctcggt
     3721 ggccttcccg gcgatctcgg ccggtgtcta cggctggccg gtgctcgatg ccgcccacgc
     3781 cgccgtggaa ggcgtgcttg ccgcggctcc cacgctcgac gcggtgcgcg acgtccggtt
     3841 cgtgcttttc gggcctacta cctacgccgc cttcgccgcc gctgtcgccg acctcgcccc
     3901 gccctccaac ccgcactgat cagtcgcaaa ccatcgcgtc gcagagcctg tctctaaggg
     3961 acgtgtctct atctgcgtct gatctggtcg agctggtggc ggtggtgctg ccgccagcgt
     4021 tgggcggggt cgaggacgcg gggtggttgc cattcgacgt agccgttggc gcgggccagg
     4081 cgtgatcgcc agccttggcg gtgggcctgg tcgtggtggt agtcgcacaa caaggcgccg
     4141 ttgcacaggt cggtgggtcc gccgtcttcc cagtggtctt ggtggtgggc ttcgcaggcg
     4201 cgggcgggtt ggtggcagcc ggggaaggtg cagccgcgat cacgcagctc gagtgcccgt
     4261 cgttggttgg tgctgaacag gcgtcgggtc cggccgaggt cgagcacggt cgaggctccg
     4321 cccatgacgg cgggcaggat gtcggccccg caggcgagcc ggcgcagctc gtggttgggg
     4381 atcttcactc cggtgtcgag caccccgttg gtcagctggt gggtcacggc gtcgtagtcg
     4441 caggtgatcg acaggaccgg tcggcggcct ccctggtcgc cgggcaggtc cgggtcgtgg
     4501 gccagggcgt ggcgggccag ccgggcgaac gcatccaacc ggcgttgcgc ccgtgtgcgc
     4561 cggtcccggc acggatcatc gtcaccctcg ctgccaggtt cgggt
//
LOCUS       DATIBB010000055        12971 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_26922, whole genome shotgun sequence.
ACCESSION   DATIBB010000055 DATIBB010000000
VERSION     DATIBB010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 12971)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12971)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12971
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26922"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..845)
                     /locus_tag="VLC50_01990"
     CDS             complement(<1..845)
                     /locus_tag="VLC50_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005478672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSF26267.1"
                     /translation="MIVFDDVSVTYAGATRPALCHVDLHVPEGELCLVIGRTGSGKST
                     LLRAVNGLVPHFTGGTLAGRVTVAGRDTRTHPPRELADVVGVVGQDPAAGFVSDLVEE
                     ELAYGMESLGLPTDVMRRRVEEVLDLMGIADLRDRPLAELSAGQAQRVAIGSVLAAHP
                     RVLVLDEPTSALDPAAAEEVLAALQRLVHDLGVTVLMAEHRLERVVQYADRVVLVPGD
                     GAPLQVGPPARVLAESPVAPPVVALGRLAGWSPLPLSVRDARRCAGPLRERLAALPRP
                     DAREKT"
     gene            complement(842..1993)
                     /locus_tag="VLC50_01995"
     CDS             complement(842..1993)
                     /locus_tag="VLC50_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019067163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HSF26268.1"
                     /translation="MTAGVLTLARQRGLALPRPLHPGAWWVWALGLAVAASRTTNPLL
                     LGLIVAVAGFVVSARRTDTPWSRSYAAYLKLGLLVIVIRVVFAILFGAPIPGTLLVTL
                     PEVSLPDWALGVRIGGPVTLETVAAAAYEGLRLATLLACVGAANSLANPKRALRILPG
                     ALYELGVAVVIAMSFSPQLLSSVGRVREARRLRGQARGGVAPVRWVRGIRRTAMPVLE
                     DALERSVELAAAMDSRGYGRRAGVPVRTRRLTATLVIGGLVGVCAGLYGLTDTGALGS
                     LPVPLLAAGLAMATVGLVLGGRRTTRSRYRPDPWAAPEWLVALSGLAAAVGVAGAAVL
                     GEPTLAGLTVPWTVPVLPLLPAAAVLVAATPAWTAPPAPTATAPTGARS"
     gene            complement(1990..2793)
                     /locus_tag="VLC50_02000"
     CDS             complement(1990..2793)
                     /locus_tag="VLC50_02000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF26269.1"
                     /translation="MITALVLAATTGLLPLAAAPVAAAPAAACPGGAGVTVVVDFGQH
                     GGTAVRCAAGDPASASEALDDADFDQARVQRQPAFLCRIDGVPSAKQDACVVTPPATA
                     YWALWTAARGGSWKFAPVGVDLVDPEPGSVVGFAFGAGAKPGVAPPAPAAASRPSSSR
                     TTKPAAAPSTRVAAPSSSASTTRAAAPSASSTPVPSVSAASTAATVSAAAPDSAAAPR
                     SLPATPAAGRAEGPSDEAAGGSLGPWVGAGLIGLVGLAAGARSWQRRRR"
     gene            complement(2790..4049)
                     /locus_tag="VLC50_02005"
     CDS             complement(2790..4049)
                     /locus_tag="VLC50_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018588608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HSF26270.1"
                     /translation="MPDSHRLRTLSGVIAVATALTLAIPFAGSASAATTAPTGPATTD
                     AADAAAGWLARQLVDGDHLTTAAGGATYPNAGLTADTVLALAAAGVGQDAAAKATAWL
                     ATPANLSGWIGDGTAESYAGSTAKAALVALTQGQSPTSFGGVDLLTRLRGLEQTSGAE
                     TGRFSDTSAYGDYSNTFGQSFAVIALERADAAGASIAAVDYLAAQQCTDGGFRLDLAA
                     GAGACASDPDATALAVQALLLAGRDDAASKGLDFLEGRQQADGGVGGVGPTAGENANT
                     TGLVAQALAAGERTDASAKAAAYLRGLQQGCAATAGARGAIAYDASGFVAGTAVAATT
                     QAVLGLTGVGMATLNASGASAEAPVLDCPGPATPAASPTASVPAPAGATLPDTGSTSG
                     PLALLGVGLVLAGAALRWATRHRGTHA"
     regulatory      complement(4215..4302)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            4586..4792
                     /locus_tag="VLC50_02010"
     CDS             4586..4792
                     /locus_tag="VLC50_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014060091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF26271.1"
                     /translation="MTWTWRYETRAGGELVPAGAASEGFPTQSDAESWLGEFWRLLLE
                     AGVDQVWLLEDGVNVYGPMSLHPD"
     gene            complement(4821..5834)
                     /locus_tag="VLC50_02015"
     CDS             complement(4821..5834)
                     /locus_tag="VLC50_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013864721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family protein"
                     /protein_id="HSF26272.1"
                     /translation="MQHRYLGRSGLKISEIAYGNWLTHGSQVEEERALACVRAALDEG
                     ITTFDTADTYANTAAESVLGRALTGQRRESLEIFTKVFWPTGPGGPNDCGLSRKHIME
                     SINGSLRRLGTDYVDLYQAHRFDYETPLEETMQAFADVVRAGKALYIGVSEWTADQIR
                     RAAALARELGVGLISNQPQYSMLWRVIEAEVVPASREVGLSQIVWSPVAQGVLTGKYK
                     IGAPVPAGSRATDEAGGGAHFVKRYLKDNVLERVAGLEPLAKEAGLSMAALAVAWVLQ
                     NDNVSAAIIGASRPEQVHENVTASGVRLEADLMARIDEVLGDVVSYDPALTRKMSPAG
                     RPS"
     gene            complement(5865..6455)
                     /locus_tag="VLC50_02020"
     CDS             complement(5865..6455)
                     /locus_tag="VLC50_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010471135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3043 domain-containing protein"
                     /protein_id="HSF26273.1"
                     /translation="MFGRRNDSKAPTTPAAAAPVHANGAGKNGGKGRPTPTRREAEAA
                     RLAARKPVRTRREALKRDRAAAREERLRTRQALMAGDERALPPRDQGPVRRYVRDFID
                     SRRTAAEFFFWIAIVVLAGSFIRPVAAYVTLFWMVALIVIVIDSMLLTVRLKSALKRK
                     FPDESLRGTTSYGLLRAMQIRRLRLPPPKVKPGATI"
     gene            6582..7376
                     /locus_tag="VLC50_02025"
     CDS             6582..7376
                     /locus_tag="VLC50_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020573317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HSF26274.1"
                     /translation="MSVMKRLSMIFRAKANKALDRAEDPRETLDYSYQKQLELLQKVR
                     RGVADVATSRKRLELQMNQLQQQTDKLDAQARQALSLGREDLAREALTRKSGLSQQVA
                     DLQVQHANLQQEEEKLTTASQRLQAKVESFRTRKETIKATYTAAEAQTKIGEAFSGIS
                     EEMSDVGLAVQRAEDKTASLQARAGAIDELIASGALEDSTGTTKDDITAELERMSSGS
                     QVELELARMRGELTAGEAPKSIDAPSATEATPATEAPQEQRQGEQP"
     gene            7373..7651
                     /locus_tag="VLC50_02030"
     CDS             7373..7651
                     /locus_tag="VLC50_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018557020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF26275.1"
                     /translation="MIVRIMGEGQYDVPGDALDDLNGLDSALEQALQTEQEAGFRAAL
                     VALLERVRAVGVRLPDESLEPSDAILPDGDASIEDVRELLGEEGLIPG"
     gene            7699..8604
                     /gene="htpX"
                     /locus_tag="VLC50_02035"
     CDS             7699..8604
                     /gene="htpX"
                     /locus_tag="VLC50_02035"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019432977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HSF26276.1"
                     /translation="MATSRYAHDRGLSARMGLTLFLLGLLYVVLIGVIFYVVQSGVVA
                     IVIAGGLLWAQWYFSDRIALYAMHGRIVSPEEAPGLHAVVDRLCALADMKKPVVAIAD
                     TDLPNAFATGRSPDRAVVCATTGLMRRLDERELEAVLSHELSHVAHRDVTVITIASFV
                     GVLAGFLTRMVMWGGVGDRRRGSDNGAPVVLIVLLVSVVVYALSFVLVRTLSRYRELA
                     ADRAGAYLTGAPSALASALTKISGDIARIPTRDLRQAEAYNAFFFTPAVSPGFSLSTI
                     FSTHPPLERRLEQLAQISAQLGRPT"
     gene            8604..9176
                     /locus_tag="VLC50_02040"
     CDS             8604..9176
                     /locus_tag="VLC50_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004991046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF26277.1"
                     /translation="MGLLDKLLGRSRPVPPDLDQLFAVPSAAITLATTTDYTPTGVGS
                     VCFRAAEGAAFASLQREIRTLLDADQGPPVEVTQDSYGFTWIVARHDPGEVAELVTDL
                     HAVNTTLADQGFGPQLLCSLVAFAEPAGRALGLVYLYKRGTFYPFAPVGDQRRDNAVE
                     LQVRGALVNDLRIEPDVTRWFPVWGAPGLG"
     gene            9247..10602
                     /locus_tag="VLC50_02045"
     CDS             9247..10602
                     /locus_tag="VLC50_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007464241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HSF26278.1"
                     /translation="MTDLPSTDELRAALARVMPGVRADLERLVRIPSVSADAFDQDHV
                     AASAEAVAALLRGEGLDVRIVAEGGRPAVIGRRTAASGTPSVLLYAHHDVQPPGDPAD
                     WDSPAFEPTERAGRLYGRGSADDKAGVMAHVAALRAWRELGVEPPVAVTVFVEGEEEI
                     GSPSLADLLAVHGPDLRADALVLADSSNWAIGTPALTTSLRGLADCVVEVSTLDHAVH
                     SGMYGGPVPDALMALVRLLATLHDDDGNVAVAGLHADTAADLDLSEERLRADAGVLDG
                     VSLVGSGSLTSRMWTRPSVTVIGIDAPSVAEASNTLRPLARAKVSLRVVPGQRAADAY
                     AALRTHLQSHAPWGAHVRVIDGELGDPVALDAVGPAYDAARQAFAQAWGNDPVDIGIG
                     GSIPFIAEFADAFPEAAILVTGVEDPDGRAHGANESLHLGEFERACLAETLLLLHLAS
                     A"
     gene            10640..11752
                     /locus_tag="VLC50_02050"
     CDS             10640..11752
                     /locus_tag="VLC50_02050"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020573322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HSF26279.1"
                     /translation="MRVLICPDKFAGTMSAPQAAAAIAEGWARARPADELVRVPLADG
                     GPGFLDVLVDALGGRLVAATVRGPLGEPTPATVLVVDDVAYVESAQACGLHLVPAGSR
                     DPAATSTYGVGQLVDVALDEGARTIVVGLGGSATNDAGAGLLAALGARPGEELAAGGV
                     ALAGLDQVDLAQPRARLAGVGLVAATDVDNPLLGLRGASQVFARQKGADDELVTCLDA
                     ALARFAAVAEPGVAPESSLAARAGAGAAGGLGYALMLLGGRRQSGIGTVLEVLGLDAL
                     IASADLVVTGEGCLDSQSLHGKVVAGVAQRARGLDRSVLVLAGLVQVGGRELSALGVT
                     AAHAVGDTPQARAQSLAHPVQTLSALAERVAGTWHR"
     gene            11882..12256
                     /locus_tag="VLC50_02055"
     CDS             11882..12256
                     /locus_tag="VLC50_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020459404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HSF26280.1"
                     /translation="MTVSPDTTQHDAQAAGAATVVLTDTAAAKVKMLLDQEGRTDLAL
                     RVAVQPGGCSGLRYQLFFDERSLDGDTVTDFGGVGVVVDRMSAPYLAGATIDFVDTIE
                     KQGFTIDNPNAGGSCACGDSFH"
     gene            complement(12321..>12971)
                     /locus_tag="VLC50_02060"
     CDS             complement(12321..>12971)
                     /locus_tag="VLC50_02060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF26281.1"
                     /translation="CPEPAGGGSPRCGGPATVTGATFGLSLEQTTDGPLLVPAWLFEV
                     AGSPDPIAVVAVDPAFWGSPDDPVPPSSDPGGSGGGSGSSGGSVGGGAGVEPVPPSGP
                     SGPPVSEPGPAVTSPTAAVSVASAKVGADERTVELVFWGAPDVDGACGAAYAASTTEA
                     SDAVVVAVNERPRPISNSVTCPAIAAERHLTVTLDAPLAQRPIVDAATGIPVPLHR"
BASE COUNT         1708 a         4832 c         4797 g         1634 t
ORIGIN      
        1 gtcttctccc gggcgtcggg ccgggggagt gctgccagcc gctcccgcag cgggccggcg
       61 caccggcggg cgtcgcgcac cgacagcggc aggggggacc agccggccag ccggcccagc
      121 gcgacgaccg gcggggcgac cggtgactcg gccagcacgc gggccggcgg cccgacctgc
      181 agcggtgccc cgtcgcccgg cacgagcacg acgcggtcgg cgtactgcac cacccgctcg
      241 agccggtgct cggccatcaa cacggtgacg cccaggtcgt gcaccagccg ctgcagcgcc
      301 gcgagcacct cctcggccgc ggccgggtcc agcgccgagg tgggctcgtc gagcaccagc
      361 acccgcgggt gggcggccag caccgacccg atcgcgaccc gctgcgcctg cccggccgac
      421 agctcggcca gcggccggtc ccgcaggtcg gcgatgccca tcaggtcgag cacctcctcg
      481 acccgtcggc gcatcacgtc ggtgggcagg ccgagcgact ccatgccgta ggccagctcc
      541 tcctcgacca ggtcgctgac gaacccggcg gccgggtcct gcccgaccac gccgaccacg
      601 tcggccagct cccgcggcgg gtgggtgcgg gtgtcccggc cggcgaccgt gacccgcccg
      661 gccagggtgc cgccggtgaa gtgcggcacc agcccattga ccgcccgcag cagcgtggac
      721 ttgcccgacc cggtgcgccc gatcaccagg cacagctcgc cctcgggcac atgcaggtcg
      781 acgtggcaga gcgccggtct ggtcgcgccg gcgtaggtga ccgacacgtc gtcgaagacg
      841 atcacgaccg tgctcccgtc ggggcggtcg cggtcggtgc cggcggtgcg gtccaggccg
      901 gggtcgcggc caccagcacc gcggcggcgg gcagcagcgg cagcaccggc accgtccacg
      961 gcacggtcag ccccgccagc gtcggctcgc cgaggacggc ggcaccggcg accccgacgg
     1021 cggcggccag cccagagagg gcgaccagcc actctggcgc ggcccacggg tcggggcgat
     1081 aacggcttcg cgtcgtacga cgtccgccga gcaccaggcc gaccgtggcc atcgccagcc
     1141 cggccgccag cagcgggacc ggcagcgacc cgagcgcacc ggtgtcggtc aggccgtaga
     1201 gcccggcgca gaccccgacc agccccccga tcaccagggt cgcggtgagc cgacgggtgc
     1261 gcaccggcac cccggcgcgg cggccgtagc cgcgcgagtc catcgcggcg gccagctcga
     1321 ccgagcgttc cagcgcgtcc tcgagcaccg gcatcgccgt acggcggatc ccgcgcaccc
     1381 agcgcaccgg cgcgaccccg cctcgtgcct gcccgcgcag ccgtcgggcc tcgcggaccc
     1441 gtcccacgct ggacagcagc tggggggaga agctcatcgc gatcaccacc gcgacgccca
     1501 gctcatagag ggcgccgggc aggatccgca gcgcccgctt ggggttggcc agcgagttgg
     1561 cggcgccgac gcaggccagc agcgtcgcca gccgcagccc ctcataggcg gcggcggcca
     1621 ccgtctccag ggtgaccggg ccgccgatgc gcacgccgag cgcccagtcg ggcagcgaca
     1681 cctccggcag ggtgaccagc agggtgcccg ggatcggggc gccgaacagg atcgcgaaca
     1741 cgacccggat cacgatgacc agcagcccga gcttgaggta ggcggcgtag gagcgcgacc
     1801 acggcgtgtc ggtgcgccgg gcgctgacca cgaacccggc caccgcgacg atcaggccca
     1861 gcagcagcgg gttggtggtg cggctggcgg ccaccgcgag cccgagcgcc cagacccacc
     1921 aggcacccgg gtgcagcggc cgggggagcg ccaggccccg ttgacgggcc agggtgagca
     1981 ccccggcggt caccgccgcc tccgctgcca cgaccgggcc ccggccgcca gcccgacgag
     2041 cccgatgaga cccgcgccga cccacggtcc gagcgatccg cccgcggcct cgtctgaggg
     2101 gccctctgcg cggccggcgg ccggggtggc cggcagcgac cgcggcgcgg ctgccgagtc
     2161 cggcgctgcg gccgagaccg tcgcggcggt cgacgccgct gacacagacg ggactggggt
     2221 cgaggacgcc gacggagctg ccgcgcgggt ggtcgaggcc gacgacgacg gggcggcgac
     2281 gcgggtcgac ggcgcggccg cgggcttggt ggtccgcgac gacgagggcc gcgaagcggc
     2341 ggccggcgct ggtggggcca cccccggctt tgcgccggcg ccgaacgcga acccgacgac
     2401 gctgcccggc tcggggtcaa ccaggtcgac cccgacgggg gcgaacttcc agctgccgcc
     2461 acgggccgcc gtccacagcg cccagtaggc agtggctggc ggcgtcacca cgcaggcgtc
     2521 ctgcttcgcg ctgggcacgc cgtcgatccg gcacaggaac gccggctgcc gctgcacccg
     2581 agcctggtcg aaatcagcgt cgtccagcgc ctcggacgcc gaggccgggt cgccggcggc
     2641 gcagcggact gccgtgccgc cgtgctggcc gaagtcgaca acgacggtga cacccgcccc
     2701 gcccgggcat gcggcggccg gcgccgcggc caccggagcg gccgccagcg gcagcagccc
     2761 ggtggtggcc gcgagcacca gcgccgtgat catgcgtgcg tgccccggtg gcgcgtggcc
     2821 cagcgcaggg cagctccggc gagcaccagc ccgacgccga gcagggccag cggtccgctc
     2881 gtgctgccgg tgtcgggcag ggtcgcgcct gcgggcgcgg ggacggaggc ggtgggcgag
     2941 gcggccggcg tggcagggcc cgggcagtcg agcacgggag cctcggcgct ggcgcccgag
     3001 gcgttcagcg tggccatccc gacgccggtc agcccgagca ccgcctgcgt ggtggccgcg
     3061 acggcggtgc cggcgacgaa cccgctggcg tcgtaggcga tcgcgccccg cgcgccggcc
     3121 gtggcggcgc acccctgctg cagtccgcgc agatatgcgg cggccttcgc cgaggcgtcg
     3181 gtgcgctcac ccgccgccag cgcctgggcg accagcccgg tggtgttggc gttctcgccg
     3241 gccgtcggcc cgaccccgcc gacgccaccg tcggcctgct ggcgtccctc gaggaagtcg
     3301 agtcccttgc tcgcagcgtc gtcgcggccg gcgagcagca gcgcctgcac ggccaacgcc
     3361 gtcgcgtcgg ggtcgctggc gcacgcaccc gcgccggcgg ccaggtccag ccggaagccg
     3421 ccgtccgtgc actgctgcgc ggccaggtag tcgacggcgg cgatgctggc gccggcggcg
     3481 tccgcccgct cgagcgcgat gaccgcgaag gactggccga acgtgttgct gtagtcgccg
     3541 tacgcgctgg tgtcggagaa ccgcccggtc tccgccccgc tggtctgctc cagcccgcgc
     3601 agccgggtga gcaggtcgac gccgccgaag ctggtcggcg actgtccctg cgtcagcgcg
     3661 accagcgcgg ccttggcggt ggagccggcg tagctctcgg cggtgccgtc gccgatccag
     3721 ccggacaggt tggccggggt ggccagccag gcggtcgcct tcgcggcggc gtcctggccg
     3781 acgcccgcgg cggcgagggc cagcacggtg tcggccgtca gcccggcgtt ggggtaggtg
     3841 gcgccccccg cggcggtggt caggtggtcg ccgtcgacga gctggcgagc cagccaaccc
     3901 gcggcggcgt cggcggcgtc ggtggtggcc ggtccggtgg gggcggtcgt cgcggccgac
     3961 gcggagccgg cgaacgggat ggccagggtc agcgccgtgg cgacggcaat gaccccgctg
     4021 agcgtccgca gacggtgaga gtcgggcatg tggtgccttt cggttgagcg gtccgtgacc
     4081 gaccccgagg caccccgaga cgtaggacgc ccccggcacg acggatgcgt gcgacggggg
     4141 cggggactcg ggtgttgacc cgggctccgt cccgcagacc tcgacggtgt tgaaggagcc
     4201 gctcgcgctc gcacaggtgc tccggctcgc cgcggtcaca cgcagtggcg tggatgcggc
     4261 ctacggttgc gggtcagcgc cggacttcga ccggcttccc ctgtgtgggc gcgtatgcag
     4321 ttgttgtgct gatggtgcgc gcgcagttga ccatggacaa gccgttgccg tcaaaacacc
     4381 atgggggtga tcaagcaatc cgcgcccgct ttgtgctcgt gatcaagcaa tccgtgtacg
     4441 gcgatcgtgc gatgagcaga caccgtggaa gatcgcaccg ttctggccgt ggtgaactga
     4501 cacgatcacg cccgcgcggc gatggcgcgg tgacgtgggg ccctgccttc gccgtacgac
     4561 gcatgcaggt ggctagcctg ggcgtgtgac gtggacgtgg cgttacgaga cccgcgccgg
     4621 gggcgagctg gtgccggccg gcgcggctag cgagggcttc ccgacccagt cggacgccga
     4681 gtcctggctc ggcgagttct ggcgcctgct cctggaagcc ggggtcgacc aggtctggct
     4741 tctcgaagac ggtgtcaacg tctacgggcc gatgagcctg caccccgact gatcgcgggc
     4801 catgcggcgg gggccagggg tcaggagggc cgacccgccg gggacatctt gcgggtcagc
     4861 gcggggtcgt aggacacgac gtcgccgagc acctcgtcga tgcgggccat caggtcggcc
     4921 tcgagccgca cgcctgaggc ggtgacgttc tcgtgcacct gctctggccg ggacgcgccg
     4981 atgatcgccg ccgagacgtt gtcgttctgc agcacccagg cgaccgccag cgcggccatc
     5041 gacagcccgg cctccttggc cagcggctcc aggccggcga cccgctcgag cacgttgtcc
     5101 ttgaggtagc gcttgacgaa gtgcgcgccc ccacccgcct cgtcggtggc ccgtgagccg
     5161 gccggcaccg gtgcaccgat cttgtacttg ccggtgagca cgccctgggc gaccggcgac
     5221 cagacgatct ggctgaggcc gacttcgcgg ctggcgggca ccacctcggc ctcgatgacg
     5281 cgccagagca tggagtactg cggctggttg gagatcagcc cgacgcccag ctcccgggcg
     5341 agggcagccg cccgtcggat ctggtcggcg gtccactcgg agacgccgat atagagcgcc
     5401 ttgccggccc gtacgacgtc ggcgaacgcc tgcatcgtct cctcgagcgg cgtctcgtag
     5461 tcgaagcggt gggcctggta gaggtcgacg tagtcggtgc cgagccggcg cagggagccg
     5521 ttgatcgact ccatgatgtg cttgcgcgag aggccacagt cattgggccc gcccggcccg
     5581 gtcggccaga acaccttggt gaagatctcc agcgactcgc gccgctgacc ggtcagcgcc
     5641 cgaccgagca ccgactcggc cgccgtgttg gcgtaggtgt cggcggtgtc gaacgtcgtg
     5701 atgccctcgt cgagcgccgc ccgcacgcag gccaacgccc gctcttcctc gacctggctg
     5761 ccatgcgtga gccagttgcc gtaggcgatc tcgctgatct tcaggccgga ccggccgagg
     5821 tacctgtgct gcatgagcgc cgaccttacg ccgggctcgc tgggctagat cgtggcgccg
     5881 ggcttgacct tgggcggtgg cagccgcagc cggcggatct gcatcgcgcg cagcagcccg
     5941 taggacgtcg taccgcgcag gctctcgtcg gggaacttgc gcttgagcgc gctcttgagc
     6001 cgcaccgtca gcagcatcga gtcgatgacg atcacgatga gcgcgaccat ccagaacagc
     6061 gtgacgtagg ccgcgaccgg gcggatgaag ctgccggcca gcacgacgat ggcgatccag
     6121 aagaagaact cggcggccgt acggcgggag tcgatgaagt cgcgcacgta gcggcgtacg
     6181 ggaccctggt cgcggggcgg cagggcccgc tcgtcaccgg ccatcagcgc ctgccgggtg
     6241 cgcagccgct cctcgcgggc ggccgcccgg tcacgcttga gcgcctcccg ccgcgtgcgc
     6301 accggcttgc gggcggccag ccgcgccgcc tcggcctccc gccgggtcgg tgtggggcgg
     6361 cccttgccgc cgttcttgcc ggcaccgttg gcatgcaccg gggccgcggc cgccggcgtc
     6421 gtgggggcct tgctgtcgtt gcgtcgtccg aacacgaggg gaagcctaac gggaaccatc
     6481 tgcccccctc gctcgtcggc gcatagggtg atctcggcag gcgaatggct gggacacgca
     6541 gccgtccgca ccgaccgacc acgaaggggg cgcgcccgcc gatgagcgtc atgaagcggc
     6601 tgtcgatgat cttccgggcg aaggccaaca aggccttgga ccgcgccgag gacccccgcg
     6661 agaccctcga ctacagctac cagaagcagc tcgagctgtt gcagaaggtc cgtcgtggcg
     6721 tggccgacgt ggcgaccagc cgcaagcgcc tggagctgca gatgaaccag ctgcagcagc
     6781 agaccgacaa gctcgacgcg caggcccgtc aggccctctc gctggggcgc gaggacctgg
     6841 cccgtgaggc gctgacccgc aagtccggcc tgagccagca ggtggccgac ctgcaggtgc
     6901 agcacgccaa cctccagcag gaggaggaga agctcaccac cgcgagccag cggctgcagg
     6961 ccaaggtcga gtccttccgc accaggaagg agaccatcaa ggccacctac accgcggccg
     7021 aggcacagac caagatcggc gaggcgttct ccggcatctc cgaggagatg agcgacgtcg
     7081 gcctggccgt gcagcgggcc gaggacaaga cggcctcgct gcaggcccgc gccggggcca
     7141 tcgacgagct gatcgcctcg ggcgcgctcg aagacagcac cggtacgacg aaggacgaca
     7201 tcaccgccga gctggagcgg atgtccagcg gcagccaggt cgagctcgag ctggcccgga
     7261 tgcgcggcga gctgaccgcg ggcgaggcac ccaagtcgat cgacgccccg tcggcgaccg
     7321 aggccacgcc ggcgacggag gccccgcagg agcagcggca gggggagcaa ccatgatcgt
     7381 gcggatcatg ggcgaggggc agtacgacgt gcccggcgac gccctcgacg acctcaacgg
     7441 cctcgactcc gcgctcgaac aggcgctgca gaccgagcag gaggccggct tccgcgcggc
     7501 gctcgtggcc ctgctcgagc gggtccgcgc cgtcggggtc cgcctgcccg acgagtcgct
     7561 cgaaccctcc gacgcgatcc tgcccgacgg cgacgccagc atcgaggacg tccgcgagct
     7621 gctcggcgag gagggcctga tcccgggctg agctcagccg acccgatcca ccgagacgaa
     7681 ggtcaccaga cgatcgccat ggccaccagc cgctacgcac atgaccgagg gctgtcggcc
     7741 cgcatgggcc tgacgctgtt cctgctcggc ctgctctacg tcgtactcat cggagtcatc
     7801 ttctacgtcg tgcagagcgg cgtggtcgcc atcgtgatcg cgggtgggct gctctgggcg
     7861 cagtggtact tcagcgaccg gatcgcgctc tatgccatgc acgggcggat cgtctccccg
     7921 gaggaggcgc ccggcctgca cgccgtggtc gaccggctct gcgcactggc cgacatgaag
     7981 aagccggtcg tggcgatcgc cgacaccgac ctgcccaacg cgttcgccac cgggcgcagc
     8041 cccgaccggg cggtggtctg cgcgaccacc ggcctcatgc gccggctcga cgagcgcgag
     8101 ctcgaggccg tgctctccca cgagctctcg cacgtcgcgc accgcgacgt caccgtcatc
     8161 acgatcgctt cgttcgttgg ggtgctcgcc gggttcctca cccggatggt gatgtggggc
     8221 ggggtgggcg accggcgccg cggcagtgac aacggcgccc ccgtcgtgct catcgtcctg
     8281 ctcgtgtcgg tggtcgtcta cgcgctgagc ttcgtgctgg tgcgcacgct ctcgcgctac
     8341 cgcgagctgg ccgccgaccg ggccggtgcc tacctcaccg gcgcaccgtc ggcgctggcc
     8401 agcgccctga ccaagatcag cggcgacatc gcacggatcc cgacgcgtga cctgcgccag
     8461 gccgaggcct acaacgcgtt cttcttcacc ccggccgtct cgcccgggtt cagcctgtcc
     8521 acgatcttct cgacccaccc gccgctggag cggcggctcg agcagctggc gcagatctcc
     8581 gcccagctgg gccggcccac ctgatgggtc tgctcgacaa gctgctcgga cgcagccggc
     8641 cggtgccgcc cgacctcgac cagctgttcg cggtgccctc ggccgcgatc accctcgcga
     8701 ccaccaccga ctacaccccg accggggtcg gctcggtctg cttccgggcc gccgagggcg
     8761 ccgccttcgc ctcgctgcag cgcgagatcc gcaccctgct cgacgccgac caggggccgc
     8821 cggtcgaggt cacccaggac agctacggct tcacctggat cgtggcccgt cacgaccccg
     8881 gcgaggtcgc cgagctggtc accgacctgc acgcggtcaa caccacgctg gccgaccagg
     8941 gcttcggccc ccagctgctc tgctcgctgg tcgccttcgc cgagccggcc gggcgggcgc
     9001 tgggactcgt ctacctctac aagcggggca cgttctaccc gttcgccccg gtgggcgacc
     9061 agcgccgcga caacgcggtc gagctgcagg tgcgcggggc gctggtcaac gacctgcgga
     9121 tcgagcccga cgtcacccgc tggttcccgg tctggggagc acccgggctg ggctgagccc
     9181 cgtccgagca atgggcggcg gcgtcacggc gggccgtctg cgctgccggg actatcgtcg
     9241 tccgccatga ccgatcttcc ctcgaccgac gagctgcggg ccgcgctggc ccgcgtgatg
     9301 cccggggtcc gggccgacct cgagcggctc gtgcgcatcc ccagcgtcag cgccgacgcg
     9361 ttcgaccagg accacgtcgc ggcctcggcc gaggcggtcg cggcgctgct gcgcggcgag
     9421 ggcctcgacg tacgcatcgt ggccgagggc ggtcgccccg cggtgatcgg gcgtcgtacg
     9481 gcggcctccg gcacgcccag cgtgctgctc tatgcccacc acgacgtgca gccgcccggc
     9541 gacccggccg actgggactc gccggccttc gagccgaccg agcgcgccgg gcgcctctac
     9601 ggccgcggca gcgccgacga caaggccggc gtgatggccc acgtcgcggc gctgcgggcc
     9661 tggcgcgagc ttggtgtcga gccgccggtc gcggtcaccg tgttcgtcga gggcgaggag
     9721 gagatcggct cgccgtcgct ggccgacctg ttggcggtgc acgggcccga cctgcgcgcc
     9781 gacgcgctcg tgctcgccga ctcctccaac tgggcgatcg gcacccccgc cctgaccacc
     9841 agcctgcgcg ggctggccga ctgcgtggtg gaggtcagca ccctcgatca cgcggtgcac
     9901 tcagggatgt acggcggccc ggtccccgat gcgctgatgg cgctggtgcg gctgctggcg
     9961 accctgcacg acgacgacgg caacgtggcc gtggccggcc tgcacgccga cacggccgcc
    10021 gacctcgacc tctcggagga gcggctgcgg gccgacgcgg gcgtgctcga cggcgtctcg
    10081 ctggtgggca gcgggtcgtt gacctcgcgg atgtggaccc gcccgtcggt gacggtcatc
    10141 ggcatcgacg ccccctcggt ggccgaggcg tccaacacgc tgcgcccgct ggcccgggcc
    10201 aaggtgtcgc tgcgggtggt ccccggccag cgcgccgccg acgcctacgc cgcgctgcgc
    10261 acgcacctgc agtcccacgc gccgtggggg gcacacgtgc gggtgatcga cggcgagctc
    10321 ggcgatccgg tcgccctcga cgccgtcggt ccggcctacg acgcggctcg ccaggcgttc
    10381 gcccaggcct ggggcaacga cccggtcgac atcggcatcg gcgggtcgat cccgttcatc
    10441 gccgagttcg ccgacgcctt cccggaggcg gccatcctgg tgaccggcgt cgaggacccc
    10501 gacgggcgcg cccacggggc caacgagagc ctgcacctgg gcgagttcga gcgggcctgc
    10561 ctggccgaga cgctgctgct gctccacctg gccagcgcct agccagctcg gccggaagcc
    10621 acgggagcag gatgtccccg tgcgcgtcct gatctgcccg gacaagttcg cggggacgat
    10681 gagcgccccg caggcggcgg cggcgatcgc cgagggctgg gcccgggcca ggccggccga
    10741 cgagctggtc cgggtcccgc tggccgacgg cgggccaggc ttcctcgacg tgctcgtcga
    10801 cgcgctgggc gggcggctgg tcgcggccac cgtgcgcgga ccgctcggtg agccgacccc
    10861 ggccaccgtg ctcgtggtcg acgacgtcgc ctacgtcgag tcggcccagg cctgcgggct
    10921 gcacctggtc ccggccggat cgcgggaccc cgcggcgacc tcgacctacg gtgtcggcca
    10981 actcgtcgac gtcgcgctcg acgagggggc ccgcacgatc gtcgtcgggc tcggcggcag
    11041 cgcgaccaac gacgcgggcg cggggctgct cgccgccctc ggcgcccgac cgggggagga
    11101 gctcgccgcc ggcggggtcg ccctggcggg cctcgaccag gtggaccttg ctcagcctcg
    11161 ggcccggctg gccggcgtcg ggctggtcgc cgccaccgac gtggacaacc ccctgctcgg
    11221 gttgcgcggg gccagccagg tcttcgcccg gcagaagggt gccgacgacg agctggtgac
    11281 ctgcctcgac gcggccctgg cccggttcgc cgcggtggcc gagccggggg tggcacccga
    11341 gagctcgttg gccgcgcggg ccggggccgg ggccgccggg ggactcggct acgcgctgat
    11401 gctgctcggg ggccgccgcc agtcggggat cggcaccgtc ctcgaggtgc tcgggctcga
    11461 cgcgctgatc gcgtcggccg acctggtggt gaccggagag ggctgcctcg acagccagtc
    11521 gctgcacggc aaggtggtcg ccggagtggc ccaacgggcc cgcgggctcg accggtcggt
    11581 gctggtgctc gccgggctgg tccaggtcgg cggtcgcgag ctgtcggcgc tcggcgtcac
    11641 ggccgcccat gccgtcggtg acaccccgca ggcgcgggcg cagtcgctcg cccaccccgt
    11701 ccagacgctc tccgcgctgg ccgagcgggt cgccggcacc tggcaccggt gacgtcgtac
    11761 ctgccctgct ctttctgtgc aagcatgagg tcaggcccgc acgggccgac cccgggggaa
    11821 tcgtcaccgc gacagcgccg ttgccccgac gaagagccga actcgacgca ggagctcgca
    11881 gatgaccgtc agccccgaca cgacccagca cgacgcccag gccgcgggtg cggccaccgt
    11941 cgtactcacg gacaccgccg ccgccaaggt caagatgctg ctcgaccagg agggccgcac
    12001 cgacctcgcc ctgcgggtcg cggtccagcc tggcggctgc tcaggcctgc gctaccagct
    12061 gttcttcgac gagcgctcgc tcgacggtga cacggtcacc gacttcggcg gggtcggcgt
    12121 cgtcgtcgac cggatgagcg ccccctacct ggccggggcc accatcgact tcgtcgacac
    12181 catcgagaag caggggttca cgatcgacaa ccccaacgcc ggcgggtcct gcgcctgcgg
    12241 cgactcgttc cactgaccgc gacccactga ccgcgacccg caccggccac tgagccggct
    12301 gggtcgctgg cggcgccgcg tcagcggtgc agcggcactg ggattcccgt tgccgcgtcg
    12361 acgatcgggc gctgtgccag cggcgcgtcc agcgtcacgg tcaggtgccg ttcggcagcg
    12421 atcgccggac atgtcacgct gttactgatc gggcgcggcc gctcgttgac cgcgaccacc
    12481 actgcgtcgg acgcctccgt ggtggaagcg gcgtacgccg caccgcaggc cccgtcgacg
    12541 tccggggcac cccagaagac cagctcgacc gtgcgctcgt ccgcgcccac tttggcggac
    12601 gcgacgctga ccgccgcggt gggagaagtg accgccgggc cgggctcgct gaccggtggg
    12661 ccggacgggc cggacggcgg gacgggctcg acgccggcgc ccccaccgac gcttcccccg
    12721 ctgctgccgc tgccaccccc gctgccgccg gggtcgctcg atggcggcac cgggtcgtcc
    12781 gggctgcccc agaacgccgg gtcgaccgcg accaccgcga tcgggtcggg ggagccggcc
    12841 acctcgaaca gccacgccgg caccagcagc ggcccgtccg tcgtctgctc gagcgagagg
    12901 ccgaaggtcg caccggtcac cgtcgccggc ccgccgcacc gtggcgaccc accgccggcc
    12961 ggctccgggc a
//
LOCUS       DATIBB010000056         7422 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_289849, whole genome shotgun sequence.
ACCESSION   DATIBB010000056 DATIBB010000000
VERSION     DATIBB010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 7422)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7422)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7422
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_289849"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1410)
                     /gene="hutH"
                     /locus_tag="VLC50_02065"
     CDS             complement(<1..1410)
                     /gene="hutH"
                     /locus_tag="VLC50_02065"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011757824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HSF26282.1"
                     /translation="MTATASPHSPADHDAVPSTVAVGTGPLTEAEVVAVSRHGAKPEL
                     TAEALAAIETSRAAIEALANDSAPHYGVSTGFGALASRHIPPAMRAQLQRSLVRSHAA
                     GSGGEVEREVVRALMLLRLSTLATGRTGIRPQTARTLEALLQHGITPVVREFGSLGCS
                     GDLAPLAHVALALMGEGEVRDPGGALRPAGEVLHEYGVVPVELAEKEGLALINGTDGM
                     LGMLCLAAADLDALLKVADVAAAMSVEGLLGTDRVFAADLQALRPHPGQAESAANLRR
                     LLDGSAIVASHRGPEDTRVQDAYSLRCAPQVAGAARDTLAHARAVAARELAAAVDNPV
                     VLPDGRVESNGNFHGAPVGYVLDFLAIPVADVASMSERRTDRFLDKARNHGLPPFLAD
                     DPGVDSGHMIAQYTQAAIVSELKRLAVPASVDSIPSSAMQEDHVSMGWSAARKLRRSI
                     DGLTRVLAIEILTAARGLDL"
     gene            1626..2423
                     /locus_tag="VLC50_02070"
     CDS             1626..2423
                     /locus_tag="VLC50_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016331655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HSF26283.1"
                     /translation="MGASSRVPAAAQVLAVLRALASAPGPTSAVALARTLGLPRSTTY
                     HLLTVLARDGFVVHLPEEHRWGLGVAAFEVGSAYLRHDPLERLARPLLTRLVDEVAVV
                     APTVGQVGVLHGRETLYLLRESPRRPVTVVTDVGVRLPAQLTASGRSLLAGLPAAQVR
                     ALFPSAAAFVDRTGRGPRTLAELRRVLSVERSRGWSVEDGEVSEGYASVAAAAHDHAG
                     RPVAAFALTFRRETCPPELWPSLADAARAAAATLSRRLGGRDVGGAP"
     gene            2434..2919
                     /locus_tag="VLC50_02075"
     CDS             2434..2919
                     /locus_tag="VLC50_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004923306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF26284.1"
                     /translation="MTIMESSPDAANPAEAVVTAVERSLTLARTWLAWDGRARVSEDG
                     DRIYTPIKAVRRIGDHLVDHLAEVEALLAGEPTQPDGWHASLVTFDSDWARFTETDLA
                     EAEQRLRRLARTFALRYAAAGPAEWDRPRGDAWTLRAIAEHLDSSWYAEQVGDLSRPV
                     A"
     gene            complement(2900..4246)
                     /locus_tag="VLC50_02080"
     CDS             complement(2900..4246)
                     /locus_tag="VLC50_02080"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HSF26285.1"
                     /translation="MSERVPPSMMSYVAARAAFVNGTDTPRDFLERCLERLDLQEPML
                     RAFAALAIPRARIAADAASRRWLAGTPLSPLDGMPIGVKDIIDTFDMPTGMGSPTSAP
                     YRPLGDAASVFAAREAGAAIVGKTKTTEFATSFPTDTRNPHDFARTPGGSSSGSAAAV
                     AAGILPVAFGTQVIGSILRPASFCGAFGFKPTFGAINRGGSLEKNLSHSCIGLIGASL
                     EDLWVTAYEIVQRVGGDPGERGLVGSATPPSAHRPPRLVSLAPESWSPTPAASEEVEA
                     LLSRCSKAGVTIITRENDERVAALERSLADVLTLARDIVAYESLWPLKPILQRDPDGL
                     SAGMREGVAKASALGIDGYRALLDRRAELRAAFAAVADDADAFVGLSAAGAAPKGLQT
                     TGDPTFTAPAAVLGIPSVTLPILHDDGLPLGLQLCAGWHEDERLFAVAKWFMQLDD"
     gene            complement(4570..5526)
                     /locus_tag="VLC50_02085"
     CDS             complement(4570..5526)
                     /locus_tag="VLC50_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018350278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HSF26286.1"
                     /translation="MKRIRVLSAIAVAAALALTGCGGDGGNPLTTGAATGPATAGTTA
                     GSTGTAAGAAIVVGSANFPENELLAQIYAKALAATGASVSTKLNIGSRETYIPALQDG
                     SIDLLPEYSGVLLQYFDKSATAVTPDDVYTALGSALPSGLIVLDRSQAEDKDAVVVTR
                     ETAEKYSLKSIADLAPVAKDLVLGGPPEWKTRPTGVPGLAKVYGLTFKEFKPLDVAGP
                     LSVQALKNGQVQAVNLFTTQSAIKANDFVVLDDPKNLFAAQNVVPLIRESKATDAVRT
                     TPNAISAALTTDELAALVKQVEVDKADAAEVAETWLQSKDLL"
     gene            complement(5526..6284)
                     /locus_tag="VLC50_02090"
     CDS             complement(5526..6284)
                     /locus_tag="VLC50_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008361906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSF26287.1"
                     /translation="MIGSLVDWLTDPAHWSGADGIPARLVEHLEYSLLATVLALLIGV
                     PAGLYIGHTGRGTFLVAGLANALRSLPTLGLLILAVLLLLPRLSGDLGFVIPSILVLV
                     ILAVPPLLTSTYAGVQSVDPAARDAAYGMGMRDGEVLRKVEVPCALPLMFSGLRSAVL
                     QVIATATVAAYVSLGGLGRFVIDGLASRDYPQVLGGAVLVAALAVVVDLLLALVARLT
                     VSRGLTGRYGRSRTLNAVPSAAVPALPAAPGESS"
     gene            complement(6281..6916)
                     /locus_tag="VLC50_02095"
     CDS             complement(6281..6916)
                     /locus_tag="VLC50_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HSF26288.1"
                     /translation="MADNADRVAGLFVDHVVLSVAPLLIGLALALPLGWLARRYRWLY
                     PPMVGATGLLYTIPSLALFVALPGILGTQILDPINVVAALTVYTVALLVRTVADGLAA
                     VPDEVTQAATAMGYRRLRRVLAVELPIATPVIAAGLRVAAVSNVSLVSVAAIIGVPQL
                     GQLLTEGFQRFYYTPIIWGIVLCALLAVLLDALILLGERLITPWRRACGAA"
     gene            complement(6934..>7422)
                     /locus_tag="VLC50_02100"
     CDS             complement(6934..>7422)
                     /locus_tag="VLC50_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF26289.1"
                     /translation="VGRDRGYRALSFVPAGRPRLHPEPTVPVGASPAEARQVALDDWV
                     LVVDDAGRPQGWAAVHAGGEPPIGRQSGRSAGAGRAGVVGGGRLERGGTVARVDGSLR
                     GLLDAALSSPSGRGVVADADGRLLGTVRASEVLATIEAEAARARADTLADTLAGSEDA
                     SS"
BASE COUNT         1125 a         2643 c         2673 g          981 t
ORIGIN      
        1 caggtcgagg ccgcgggcgg cggtgaggat ctcgatggcc agcaccctcg tcagcccgtc
       61 gatcgaccgg cgcagcttgc gcgcggccga ccagcccatc gagacgtggt cctcctgcat
      121 cgcgctggag gggatcgagt cgacgctggc tggcaccgcg agccgcttga gctcggagac
      181 gatcgcggcc tgggtgtact gcgcgatcat gtgcccggag tccacgccgg ggtcgtcggc
      241 caggaacggc ggcaggccgt ggttgcgggc cttgtcgagg aaccggtcgg tgcgccgctc
      301 cgacatcgac gccacgtcgg cgaccgggat cgcgaggaaa tccaacacat agccgaccgg
      361 ggcgccgtgg aagttgccgt tggactccac ccgcccgtcg ggcagcacca ccgggttgtc
      421 gaccgcggcg gccagctcgc gggccgcgac cgcccgggcg tgcgccaggg tgtcgcgggc
      481 cgcgccggcc acctgggggg cgcagcgcag cgagtaggcg tcctgcaccc gcgtgtcctc
      541 gggaccgcgg tggctggcga cgatggccga cccgtcgagc agccgccgca ggttggccgc
      601 cgactcggcc tggccggggt gcgggcgcag cgcctgcagg tcggcggcaa agacccggtc
      661 ggtgccgagc agcccctcga cgctcatcgc cgcggcgacg tcggccacct tgagcagcgc
      721 gtcgaggtcg gccgcggcca ggcagagcat gccgagcatg ccgtcggtgc cgttgatgag
      781 cgccagcccc tccttctcgg ccagctcgac cggcaccacg ccgtactcat gcagcacctc
      841 gccggcaggc cgcagcgcgc cgccggggtc gcgcacctcg ccctcgccca tcagcgccag
      901 cgcgacgtgg gccagcgggg ccaggtcgcc ggagcagccg agcgagccga actcgcgcac
      961 caccggcgtg atgccgtgct gcagcagggc ttccagggtg cgcgcggtct gcggccggat
     1021 cccggtacgc ccggtcgcga gcgtggacag ccgcagcagc atcagcgcgc gcaccacctc
     1081 gcgctcgacc tctcccccgg agcccgcggc gtgcgagcgg accaggctgc gctgcagctg
     1141 agcccgcatc gccggcggga tgtgccggct ggccaacgcg ccgaacccgg tggacacgcc
     1201 gtagtgcggc gcgctgtcgt tggctagcgc ctcgatcgcg gcccggctgg tctcgatggc
     1261 ggccagcgcc tcggcggtca gctcgggctt ggccccgtgg cgggagaccg cgacgacctc
     1321 agcctcggtc agcggcccgg tgcccaccgc gacggtgctc gggacggcgt cgtggtcggc
     1381 gggcgagtgc gggctggcgg tggcggtcac gggcgccatt ccacgccgta cggcgcctcc
     1441 ggtcgaggac cctgccacca ttgctgtctg ggaccccaga cgtccgcagg aatgaccacg
     1501 ccaagtaggc gtcgtggcac ttggcaaggc gtgcgcgcct tgccaagtga cagaacgcct
     1561 tgcctagggc gaggcaggcc cgcccaggcg ccaccgacgc acgaccgtca gagggaagga
     1621 ccaacgtggg agccagctcg cgggtgccgg ccgcggcgca ggtcctcgcg gtcttgcggg
     1681 cgctggccag cgcaccgggt ccgacgagcg ccgtcgcgct ggcccgcacg ctcgggctcc
     1741 cgcggtcgac gacctaccac ctgctgacgg tgctcgcccg cgacgggttc gtcgtacacc
     1801 tgccagagga gcaccggtgg gggctagggg tcgccgcatt cgaggtgggc tcggcctacc
     1861 tgcgtcacga cccgctcgag cggctggccc ggccgctgct gactcggctg gtcgacgagg
     1921 tggccgtggt cgcgccgacg gtcgggcagg tcggagtgct gcacgggcgg gagacgctct
     1981 acctgctgag ggagtcgccg cgccgaccgg tcacggtggt caccgacgtc ggcgtccggc
     2041 tgccggccca gctgaccgcg tcggggcgca gcctgctcgc cgggctgccg gccgcccagg
     2101 tgcgggcgct gttcccgtcg gcggccgcgt tcgtcgaccg caccggccgg gggccgcgca
     2161 ccctggccga gctacgccgg gtgctgtcgg tggagcgctc acgcggctgg tcggtcgagg
     2221 acggcgaggt cagcgagggc tacgcctcgg tggccgcggc cgcccacgac catgctggcc
     2281 ggccggtggc ggcgttcgcg ctgacgtttc gccgcgagac ctgcccaccg gagctctggc
     2341 cgtcactcgc cgacgcggca cgggcggcgg ccgccacact gagccgacgt ctgggcgggc
     2401 gcgatgtcgg cggggcgccg taacgtgccg gtcatgacga tcatggagag cagccccgac
     2461 gccgccaacc ccgccgaggc cgtcgtcacc gctgtcgaac ggtcgttgac cctcgctcgc
     2521 acctggctgg cctgggacgg tcgcgctcgg gtcagcgagg acggcgaccg gatctacacc
     2581 ccgatcaagg cggtacgacg catcggcgac cacctcgtcg accacctggc cgaggtggag
     2641 gccctgctcg ccggcgagcc gacccagccc gacggctggc acgccagcct ggtcaccttc
     2701 gacagcgact gggcccggtt caccgagacc gacctggccg aggccgagca gcggctgcgc
     2761 cggctggccc gcacgttcgc gctgcgctac gccgcggccg gcccggccga gtgggaccgt
     2821 ccccgcggtg acgcctggac gctgcgcgcg atcgccgagc acctggacag ctcgtggtac
     2881 gccgagcagg tcggcgacct cagtcgtcca gttgcatgaa ccatttggcc actgcgaata
     2941 gacgctcgtc ctcatgccaa ccggcgcaaa gctgaagacc caaggggagc ccgtcgtcgt
     3001 gaaggatggg gagggtcacg gacgggatgc caagcaccgc ggctggcgca gtgaaggtcg
     3061 gatcgccggt cgtctgcagg cccttcggcg cagcgccggc ggctgacagg ccgacgaagg
     3121 catcggcatc gtcagcgacc gccgcaaagg ccgcccgcag ctctgctcgg cggtccagca
     3181 gggcccgata tccgtcgatg cccagggccg atgccttggc cactccttcg cgcatccctg
     3241 cactgagtcc gtccgggtcg cgttgaagga tcggcttgag cggccacaga gattcatagg
     3301 ccacgatgtc cctggcgagg gtcaggacat cagccaacga ccgctcgagt gcggctacac
     3361 gctcatcatt ctcgcgagtg atgatcgtca cccccgcttt gctgcaccga gaaagcagag
     3421 cttcgacctc ctcgcttgcc gccggggtcg ggctccatga ctccggcgca agactgacta
     3481 gccgtggtgg ccgatgcgcg cttggcggcg tggccgagcc cacgagtcca cgctctcccg
     3541 ggtcgcctcc cacgcgttgc acaatttcgt aggccgtcac ccacagatct tcgaggctgg
     3601 ccccgatcaa gccgatgcag ctgtggctca aattcttctc gagacttccg ccgcggttga
     3661 tcgcgccgaa cgtcggcttg aacccgaagg cgccacaaaa gctggccgga cgaaggatcg
     3721 agccgatgac ctgtgttccg aacgcgaccg gcaggattcc tgcggcgacc gcggccgcgg
     3781 accccgagct tgatccgccg ggcgtgcgcg caaagtcgtg cgggttccgg gtgtccgtcg
     3841 ggaagcttgt cgcgaactcg gtggtcttgg tcttgccaac tatcgccgcg ccggcttcac
     3901 gtgccgcgaa aaccgaagcc gcatcaccca acggtcggta cggcgcagag gtgggcgacc
     3961 ccatgccggt cggcatgtcg aaggtgtcaa tgatgtcctt gaccccgatc ggcatcccgt
     4021 ccaaggggga caagggcgtt cctgcgagcc agcggcggct cgcggcgtcg gccgcgatac
     4081 gtgctctcgg aatggcgagc gcggcaaacg cccgcagcat cggctcctga aggtcaagtc
     4141 gttcgaggca tctctccaga aagtcacgag gcgtgtccgt gccattcacg aaggccgcgc
     4201 gagccgcgac ataggacatc atcgacgggg gcacacgctc cgacatgatt cggcctccac
     4261 cttcatctcg tcgatgaatg tctcgacgga acgtgcctcc cagcccccat gacggacgcg
     4321 aacacgcgtc aaactggaat gcgtgactga catgctagat gtgctgggat gccgagtcaa
     4381 tagtcaccgg cagcgggcac atcgtggaag ttcacagcgc tctggcacac gcggattgac
     4441 acgatcatgc ccagccccgt gcacgcggta caccaattgc ttgatcacca cacgaaagtg
     4501 gacaccgatt gcttgatcac cgacaggggg cgggcacgga ttgcttgatc acggaggaag
     4561 tggggagggc tagaggaggt ccttggactg cagccaggtc tcggcgacct cggcggcgtc
     4621 ggccttgtcg acctcgacct gcttgaccag cgcggccagc tcgtcggtgg tcaacgccgc
     4681 ggagatcgcg ttgggcgtgg tgcgcaccgc gtcggtggcc ttggactcac ggatcagcgg
     4741 gacgacgttt tgcgcggcga aaaggttctt ggggtcgtcg agcaccacga agtcgttagc
     4801 cttgatcgcc gactgagtgg tgaacaggtt gaccgcctgg acctgaccgt tcttcagcgc
     4861 ctgcaccgac agcggccccg ccacgtcgag cggcttgaac tccttgaacg tcaacccgta
     4921 gaccttcgcc agcccgggca caccggtcgg gcgggtcttc cactccggcg gcccgccgag
     4981 cacgaggtcc ttggcgaccg gggccaggtc ggcgatggac ttgagcgagt acttctcggc
     5041 cgtctcgcgg gtcaccacca ccgcgtcctt gtcctcggcc tgcgaccggt cgagcacgat
     5101 caggccggag ggcaacgcgg agcccagagc ggtgtagacg tcgtcggggg tcaccgcggt
     5161 cgccgacttg tcgaagtact gcagcagcac gccggagtac tccggcagca ggtcgatcga
     5221 gccgtcctgc agcgccggga tataggtctc gcggctgccg atgttgagct tggtgctgac
     5281 cgaggcgccc gtggccgcca acgccttcgc gtagatctgg gcgagcagct cgttctccgg
     5341 gaagttggcc gacccgacga cgatcgccgc cccggcggcc gtgcccgtgg accccgcagt
     5401 ggtgccggcg gtggcgggac cggtggccgc cccggtggtc agcggattgc cgccgtcacc
     5461 gccgcacccg gtcagcgcga gggccgcggc gacggcgatg gcggacagga ctcggatgcg
     5521 cttcatcagc tcgactcccc tggtgcggct ggtagggctg gaacggcggc ggaaggtacg
     5581 gcgttcaacg tccgtgaccg accgtaacgc ccggtgagcc cgcgcgagac ggtcaaccga
     5641 gcgacgaggg cgagcagcag gtcgacgacg acagccagcg cggccaccag caccgccccg
     5701 ccgagcacct gcgggtagtc gcggctggcc agcccgtcga tgacgaaccg gcccagcccg
     5761 cccagtgaca cgtaggcggc cacggtggcg gtggcaatca cctgcagcac cgcgctgcgc
     5821 agcccggaga acatcagcgg cagcgcgcac ggcacctcga ccttgcgcag cacctccccg
     5881 tcgcgcatcc ccatgccgta ggcggcatcg cgcgcggccg ggtcgacgct ctgcaccccg
     5941 gcgtaggtgc tggtcagcag cggcggcacg gccaggatca ccagcacgag gatcgacggg
     6001 atgacgaagc cgaggtcacc ggacaggcgc ggcagcagga ggagtacggc gaggatgagc
     6061 agccccagcg tgggcagcga ccgcagcgcg ttggccagcc cggcgacgag gaacgtgccc
     6121 cgcccggtgt ggccgatgta gagaccggcg ggtacgccga tgagcagcgc gagcaccgtc
     6181 gccagcaggg agtactcgag gtgctcgacg aggcgcgccg ggatcccgtc ggccccgctc
     6241 cagtgcgccg ggtcggtgag ccagtcgacc agcgagccga tcatgcggca ccacaggcgc
     6301 gacgccacgg ggtgatcagc cgctcgccga gcaggatcag cgcgtcgagc aagaccgcca
     6361 acagggcgca cagcactatg ccccagatga tcggcgtgta gtagaagcgc tggaagccct
     6421 cggtgagcag ctggcccagc tggggcaccc cgatgatcgc ggcgacgctg accaggctga
     6481 cgttgctcac cgcggccacc cgcagtccgg cggcgatcac cggcgtggcg atcggcagct
     6541 cgacggccag cacccggcgc agccgccggt agcccatcgc ggtcgccgcc tgggtcacct
     6601 cgtcgggcac cgcggccagc ccgtcggcga cggtgcgcac cagcagcgcc acggtgtaga
     6661 cggtcagcgc ggcgaccacg ttgatcgggt cgaggatctg ggtgccgagg atgccaggca
     6721 gcgcgacgaa aagcgccagt gacgggatcg tgtagagcag cccggtggcc ccgaccatcg
     6781 gcgggtagag ccagcggtag cggcgggcca gccagcccaa cggcagcgcc agtgccagcc
     6841 cgatcagcag cggggccacc gagagcacca cgtggtcgac gaacagcccg gccacccggt
     6901 cggcgttgtc ggccagccag ccgaagaagt cgctcacgag ctcgcgtcct cggagccggc
     6961 caaggtgtcg gccagcgtgt cggcgcgggc ccgggcggcc tcggcctcga tggtggccag
     7021 cacctccgag gcgcgcaccg tgccgagcag ccgaccgtcg gcgtcggcga ccacgccgcg
     7081 cccggacggc gaggacagcg ccgcgtcgag cagcccgcgc aacgacccgt cgacccgggc
     7141 cacggtgccg ccacgctcga gccggccccc gccgaccacc ccggcccgac cggcaccggc
     7201 cgaccggccg ctctggcgtc cgatcggcgg ctcgccaccg gcgtgtacgg cggcccagcc
     7261 ctgcggccgc ccggcgtcgt cgacgaccag cacccagtcg tcgagggcca cctggcgggc
     7321 ctcggccggc gaggcaccca ccggcacggt cggctcggga tgcagccgcg gccggccggc
     7381 gggcacgaac gagagcgcgc gataaccccg gtcgcggccg ac
//
LOCUS       DATIBB010000057         2868 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_1605441, whole genome shotgun sequence.
ACCESSION   DATIBB010000057 DATIBB010000000
VERSION     DATIBB010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2868)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2868)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2868
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1605441"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..947
                     /locus_tag="VLC50_02105"
     CDS             <1..947
                     /locus_tag="VLC50_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020541672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HSF26290.1"
                     /translation="EADAVVIATGARPRRVAAEHSMALRTVEDALALRARLVPGARVV
                     VVGAGWIGAEVATAAAAAGASVDVVEAGPTPLAGAMPAEIGRLTLPWYDAAGVHVHLG
                     ARVERVERQGAELTVALADARVLRADLVVEGVGVRPATDWLADSGIALDAHGAVVTDS
                     RLRASRPGVWAVGDVASWWSPRFARTMRVEHWDEALHAPEVVAASIIAGGSDAGPEHD
                     PVPYFWSEQLGHYVQYAGHHPAGTRLVHRGDPADPGGWTACWLAPGGDGERLVAVLAV
                     DRPRDLMQGRKLIAAGAAVDVARLADPSIALREAAQPA"
     gene            989..1363
                     /locus_tag="VLC50_02110"
     CDS             989..1363
                     /locus_tag="VLC50_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019877432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rv2175c family DNA-binding protein"
                     /protein_id="HSF26291.1"
                     /translation="MTAPTSSSPAPDDLDALVGEWLTLPDVAERLGVDAARARQLVAD
                     RQLVAVRREGVLRVPAAFVVDGKVLKGLPGTLTLLADAGFDDVEALRWLFTDDDLPGS
                     PVQALTQNRGTEVRRRAQALGF"
     gene            1380..1796
                     /locus_tag="VLC50_02115"
     CDS             1380..1796
                     /locus_tag="VLC50_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018635865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lycopene cyclase domain-containing protein"
                     /protein_id="HSF26292.1"
                     /translation="MRHLAYLGMLAFCVLGTVWLELLLGVRVLRRLRRLLLTLVPVLV
                     VFVAWDLYAIARGHWDFDRAQTVGVVLPGGLPLEELLFFVVIPLCALLTLEGVRALTG
                     WPVGDGGPDGVRRPVLRRSRRTDADRSAGARPGDRG"
     gene            1793..2098
                     /locus_tag="VLC50_02120"
     CDS             1793..2098
                     /locus_tag="VLC50_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014688677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lycopene cyclase domain-containing protein"
                     /protein_id="HSF26293.1"
                     /translation="MSYTALAVVGVLATVVLDVWVLRTRLLARRAFWAAYVILVFFQL
                     LTNGWLTGRDIVTYDPAAIVGLRVAFAPVEDLLFGFALVVQTMAWWVWWGRRGVQRD"
     gene            complement(2229..>2868)
                     /locus_tag="VLC50_02125"
     CDS             complement(2229..>2868)
                     /locus_tag="VLC50_02125"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011435825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HSF26294.1"
                     /translation="TVGRWDIPHEHVEAFLRSMRQDLTVSSYATYDDLRDYMHGSAAV
                     IGLEMVPILEPLDTAAHRHAAALGEAFQLTNFIRDVGEDLRRGRVYLPQEDLRRFGVT
                     RDALAAGVVTEPIRALLRFQIERTRGLYAIAREGIPMLHPTSRDCIATAVTLYGELLE
                     AVERADYQVMTQRVRVPISRRLQVALPGLVRSRAARRSEGAWRLAPTPPLSR"
BASE COUNT          311 a         1004 c         1112 g          441 t
ORIGIN      
        1 tcgaggccga cgcggtggtc atcgccaccg gtgcgcggcc gcggcgggtc gcggcggagc
       61 actctatggc gctgcgcacc gtcgaggacg cgctggccct gcgtgcccgg ctcgtgcccg
      121 gggcgcgggt cgtggtcgtc ggggccggct ggatcggggc cgaggtggcg accgcagcgg
      181 cggcggccgg tgcctcggtg gacgtcgtcg aggccggccc gaccccgttg gcgggcgcga
      241 tgcccgccga gatcggccgg ctcaccctcc cgtggtacga cgcggccggg gtgcacgtgc
      301 acctgggtgc gcgagtcgag cgggtcgagc gccagggcgc ggagctgacg gtggccctgg
      361 ccgacgcccg agtgctgcgg gccgacctcg tcgtcgaggg ggtcggggtg cgcccggcga
      421 ccgactggct cgccgacagc gggatcgcgc tcgacgcgca cggcgcggtc gtgaccgaca
      481 gccggctgcg ggcctcgcgc ccgggcgtct gggcggtcgg cgacgtggcc agctggtggt
      541 cgccgcggtt cgcccggacg atgcgggtgg agcactggga cgaggcgctg cacgcccccg
      601 aggtggtcgc cgcctcgatc atcgctggcg ggtccgatgc cggcccggag cacgacccgg
      661 tgccctactt ctggagcgag cagctcggcc actacgtgca gtacgccggc caccacccgg
      721 cgggcacccg gctcgtccac cgaggcgacc ccgccgaccc cggcgggtgg accgcctgct
      781 ggctcgcccc cggcggtgac ggcgagcggc tggtcgccgt actcgccgtg gaccggcctc
      841 gtgacctcat gcagggtcgc aagctcatcg ctgcgggcgc ggccgtcgac gtggcccggc
      901 tcgcggaccc ctccatcgcc ctgcgcgagg ccgcgcagcc ggcataggcc cgagcacgtc
      961 ggatgatcgg cgtggcaggc ttggccgcgt gaccgcacca acctcgtcca gccccgcccc
     1021 cgacgacctc gacgccctgg ttggcgagtg gctgacgttg cccgacgtcg ccgagcggct
     1081 cggggtcgac gccgcgcggg ctcgccagct ggtcgccgac cgccagctgg tcgccgtacg
     1141 gcgtgagggg gtgctgcgcg tgccggcggc cttcgtcgtc gacggcaagg tgctcaaggg
     1201 gctgcccggc acgctgacgc tgctggccga tgccggcttc gacgacgtcg aggcgctgcg
     1261 atggctgttc accgacgacg acctgcccgg ctcaccggtg caggcgctga cccagaaccg
     1321 cggcaccgag gtccgccggc gcgcccaggc tctggggttc tgagtctctc ggggtcccgg
     1381 tgcgccacct cgcctacctg ggcatgctcg cgttctgcgt gctgggcacg gtctggctcg
     1441 agctgctgct cggggtgcgg gtgctgcgcc gactgcgccg cctgctgttg acgctggtcc
     1501 cggtgctggt cgtgttcgtc gcctgggacc tctatgcgat cgcgcgcggg cactgggact
     1561 tcgaccgcgc gcagaccgtg ggggtcgtgc tccccggggg cctgccgctg gaggagctgc
     1621 tgttcttcgt cgtgatcccg ttgtgcgcgc tgctcaccct cgagggcgtg cgggccctga
     1681 ccgggtggcc ggtcggcgac ggcgggcccg acggcgtacg acgaccggtg ctgcggcgga
     1741 gtcgtcgtac cgatgctgac cgttccgccg gcgcgcgtcc cggggaccgg gggtgagcta
     1801 caccgcgctg gccgtcgtcg gtgtgctcgc gaccgtggtg ctggacgtgt gggtgctgcg
     1861 gacccggctg cttgctcggc gggcgttctg ggcggcctac gtgatcctcg tgttctttca
     1921 gctgctcacc aacggctggc tgaccgggcg cgacatcgtg acctacgacc cggccgcgat
     1981 cgtggggctg cgggtagcct tcgcgccggt cgaggacctg ctgttcggct tcgcgctggt
     2041 ggtgcagacg atggcgtggt gggtctggtg gggccggcgc ggcgtccagc gcgactgacc
     2101 ccgccccttt gtccgtgatc aagcaatccg tgtacgccgc tgtgccgtca tcgtgtcaat
     2161 ttgcagcgct gcggcacgca cgatcttccg cgatgatgtg gcgttgaccg cgtgcagggt
     2221 gtgccagatc accgtgagag gggtggggtc ggggccagcc gccaggcgcc ctcggagcgg
     2281 cgggccgcgc gactgcgcac gaggccgggc agggccacct gcagccgccg actgatcggc
     2341 acccgcacgc gctgggtcat cacctggtag tcggcgcgct cgaccgcctc gagcagctcg
     2401 ccgtagaggg tcaccgccgt ggcgatgcag tcgcgactgg tcgggtgcag catcgggatg
     2461 ccctcgcggg cgattgcgta gagcccacgc gtccgctcga tctggaagcg cagcagcgcc
     2521 cggatcggct cggtgaccac acccgcggcc agcgcgtcgc gggtcacgcc gaaccggcgc
     2581 aggtcctcct gtggcaggta gacccggccg cggcgtaggt cctcgcccac gtcacggatg
     2641 aagttggtca gctggaacgc ctcgccgagc gccgcggcat gcctgtgcgc tgcggtgtcg
     2701 agcggctcga gaatcgggac catctcgagc ccgatcaccg ccgcggaccc gtgcatgtag
     2761 tcgcggaggt cgtcgtacgt cgcgtagctc gacaccgtga ggtcctggcg catggagcgg
     2821 aggaacgctt cgacgtgttc gtgggggatg tcccaccggc cgaccgtg
//
LOCUS       DATIBB010000058        12499 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_421732, whole genome shotgun sequence.
ACCESSION   DATIBB010000058 DATIBB010000000
VERSION     DATIBB010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 12499)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12499)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12499
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_421732"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1505
                     /locus_tag="VLC50_02130"
     CDS             <1..1505
                     /locus_tag="VLC50_02130"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HSF26295.1"
                     /translation="ALAEALARSDPRLRYLRLPINSGAAGRPRNEGIDATRAPYVMFL
                     DSDDELEPHACADLLRAVRETDAELASGLCVRVLVQDGHAETPWYPRLYRERLTYDDV
                     AERPGLLFDTNSTNKIYRRSFLERTGLRYPEGRTYEDLLFTTAAYCLARRITLTPERV
                     YRWYVDERADRLSVTNRRAELANLAARLSIHRDIDAWLLAHGRAALKVHKDVKFLQHD
                     LRLYLGDLPRRDPAYQQEFLDQAGDYLAGLTPEGLDLAGTMLRVAAYLVRAHDLDGVL
                     AVSAWLGQRQRLTIDLVADGDQVFWGERHLDTPEGRHALDVTDLGLADRPLSKLHLQT
                     TATSVAVDGSALTVRGRTRNQLDKVGAGSAELALILTARGSHRQCAVPMSEICHRGRS
                     IAWGASVPVADLLGVIEADERVWDVALRIRVGEEVALATVLVDGVALPGGLPVRVRRR
                     LLAGDTLVPYATVNGNLALRLVASRPSTRVVRALTQRLLHTFARRPRRRG"
     gene            1563..3350
                     /locus_tag="VLC50_02135"
     CDS             1563..3350
                     /locus_tag="VLC50_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019890649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5941 domain-containing protein"
                     /protein_id="HSF26296.1"
                     /translation="MTSTKEAVLVRVLVIGEAGLDQVREQLATLGLRDATAVSPAAVD
                     QLTALTGTGEPVLLVDAGWVGHTHALRLVGTDPRPGVRVAAGLLAASPDHLDDLLAAA
                     RQAGRLTPDALADTLRADHVTLIEPVNGSLVSAVATDPAHRAALADERDGVDEEALRL
                     AGAVKAHDGFFTTHFVSPYSRYLARWFARLGWRPNHVTMLSMLVALLAAALCATGQRW
                     GYVAGAVLLQASFTLDCVDGQLARYTLDFSRVGAWLDATFDRAKEYALFAGLAIGSAR
                     TGTDVWLLAGAAMSLQTVRHSLDFAFAAASDPTARSTSAARLSSRLDAVGWTVWARRM
                     VILPIGERWALISVLVALTSPRTTFVVLLVAGTLAGAYSLGGRVLRSRGLATSPQAAS
                     RLRAMADGGEPARLSAATRLGTLPASPLALAGALALVAGLVATGTAGDAGVVLLVGAL
                     GYVVLAGSGSAGRFAGPLGWLVVPLVRASEYGATLALTAVIAPEALPAAFGLLAAVAY
                     HHYDTVYRLRGDAAPPPRLVVALSGGHDGRVLLLCALALAGAATLRPGLVVVAVFIAA
                     VVTIESVRTWTRPDTARAGRPLDDAGDLA"
     gene            3347..4078
                     /locus_tag="VLC50_02140"
     CDS             3347..4078
                     /locus_tag="VLC50_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012919224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocholine cytidylyltransferase family
                     protein"
                     /protein_id="HSF26297.1"
                     /translation="MIGLVLAAGAGRRLRPYTDTLPKTLVPIDDETTVLDIALANFAE
                     IGLTDVAVVVGYAADVVRDRQAALEKRHGVTLELVHNPKAEEWNNAYSLWCARDLLGQ
                     GVLLANGDTVHPVSVERTLLDARGPALLLALDTVKSLADEEMKVQWSPEQGLTRITKL
                     MDPATAYGEYIGVTLIEPDAAAPLTEALEATWRRDPNLYYEDGYQELVDRGFRVDVAP
                     IGTVDWVEIDNHADLARAREIVCRS"
     gene            4066..5127
                     /locus_tag="VLC50_02145"
     CDS             4066..5127
                     /locus_tag="VLC50_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020552286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HSF26298.1"
                     /translation="MPLLTRMVTAPLIVDVRRGAFDELATVLADQRISTAGRVAIVLG
                     PHSGQRLRPRLEHQRLHADYFTVSAGTVDAAVDLVEAARRSTYDAIVGIGGGRVLDVT
                     KFAAARLGLPMVAVATNLAHDGIGSPVAILDNDRGRGSYGVPAPIAVLVDLDLVRQAP
                     RASVAAGIGEALSNLSAVADWELAHEVTGEAIDGLAVTMSRTAARSLLHDPGDLDSDA
                     FVALLAEALVLSGLAMVVAGTSRPCSGACHEISHAIDLLHPDRAGAHGEQVGVGAAFA
                     TWLRGDHELSATIVQVLRRHGLPVGPADLGFTDAEFVEILAFAPQTRPGRYTILEHLD
                     LDAAGIADAVRGYASAIGC"
     gene            5105..5869
                     /locus_tag="VLC50_02150"
     CDS             5105..5869
                     /locus_tag="VLC50_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017618795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HSF26299.1"
                     /translation="MPAPSVAELRRVAQPEGVLGRRSGEHWAGRLYMRHVSIYATRVL
                     VALRVAPNTVTAGMIVIGLLAGPALLLPGVWGALLAALLVQLYLLLDCSDGEVARWTG
                     RTSISGVYLDRVGHYLCEAAFMVGLGLRADDLEVGGYAMLGTLTALGVVLIKAETDLV
                     DVARARAGLPAAEDASAAPRSSGVALARRLAAGLRLHRLLGAVEASLLVLAVAVVDAV
                     RGDLRATQVTVVVLVVVAGLQLVAHLVSILASRRLA"
     gene            5908..6738
                     /locus_tag="VLC50_02155"
     CDS             5908..6738
                     /locus_tag="VLC50_02155"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012854417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HSF26300.1"
                     /translation="MGDRPAELARAVHSVTSQHGAPIEVVVVGNGVAVPAPAPAVRTM
                     SLPENVGVPAGRNVGWAQTSADVVIFLDDDGWFPEPDLGERLRAAFAADPRLGIVAFR
                     VTDPDDASTQRRHVPRLVVGDPMRSSEVTTFVGGACAVRRKVLEEVGGLPDAFFYAHE
                     ETDLAWRALDAGWRIRYDAGALMHHPGTSPTRHASYLRLNARNRVWLARRNLPAALAV
                     LYLLCWVALTVVRVRQPAALKAWFAGFVEGLRTDAGPRRPLRWATAWRMTRLGRPPLL
                     "
     gene            6777..7715
                     /locus_tag="VLC50_02160"
     CDS             6777..7715
                     /locus_tag="VLC50_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020576877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSF26301.1"
                     /translation="MTDPARQVYADGAAKVATGADDSSGGPAAPSAAEVAAHHGLRRS
                     AARLPLSDYVRELWARRHFIVAFASARNIAVYTNARLGQVWQVLTPLLNAAVYYLVFG
                     LIIKTRGGIPNYIAFLVIGIFIFTYTQRSVLSGSKAINGNLDLIRALHFPRATLPFAY
                     TIIELQQLAISMVVMLAIVLATGEPVTLAWLLIAPALLLQTLFNIGASLFVARLAAKV
                     TDVTQLLPFILRTWLYVSGVFYSIAFFTESAPKWVQTILQANPGAVYIEIIRKVLIQD
                     HETAGPIWAYAVVWAVVAFVGGFLYFYRAEETYGRG"
     gene            7723..8451
                     /locus_tag="VLC50_02165"
                     /pseudo
     CDS             7723..8451
                     /locus_tag="VLC50_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020576876.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(8723..10462)
                     /locus_tag="VLC50_02170"
     CDS             complement(8723..10462)
                     /locus_tag="VLC50_02170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF26302.1"
                     /translation="MSQSSPGFEDALTQLQREVSLWRESYDRLARSRPVQTAQRLRAL
                     AARAGIGSGARTGLDTEARARAIAKALGAPARRPDGDDLPPASRRSGSGRERIRVGIA
                     SIPQREAGLAKVVEALYDQADEICVHLNGYDAIPSFLRDDAKLRAVSGVDLGDRGKFR
                     FIDGFSGYYVTVDDDITYPSFYVEHLIDGIERYGRRAIVGWHGSILDPAITDFYDHSR
                     RRVLEFSKDVAKDTFVHVLGTGAAALHTDTLALTLADFPAPNMADIFMALQALRHDVP
                     LVTLAHRAGWAAGIPFPRAATIWGASTDANASRFDTRDTVREHLPVLAGSQIRTTPAL
                     LTRPPLRLSVAPSTARDQADADALEAALRHALAPLGVDVDRDDAQGAYGLRVRVDAED
                     AAGTVTLDVTRSLGGFVLDPGGRALRVDDAPGTGAAPRPLAFGQTAGVLLGHAATLAN
                     SQALAEPVSEWVQALQALLPGAPLLVVGDPDAVAALDDRLTAVAPAAEPTHRLAIDVA
                     GTGTAGAPAVRSPAELTDVLPALYADPTLWRWHRDAVASARSRESRGARAARLYAGLL
                     AMAGGVSRSSSDG"
     gene            10543..>12499
                     /locus_tag="VLC50_02175"
     CDS             10543..>12499
                     /locus_tag="VLC50_02175"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HSF26303.1"
                     /translation="MSPLLRNPRFYALVAVITLATVAAAVLINPTAAAAVVAAVVGVT
                     LAVTLRALRLSQQQAAATRVASAKAAAAMQTDLGRLRAELDTVHDDVTQLLLRPVPER
                     SEANDGLLVTIGAARAAAVSATLAELPVGTRVVDLLGCSPAEVDGLHADDRAALALVA
                     DSVAGLRLALPALESLGSARRLVIAIDDVAARRGAVLAAANPGLLAPTSLQHAAAAGG
                     GSVVRVGFAAPVRVHRVAATLLTGWASGRRRIDAAGLRVAVMSWAALPWVAGDPSART
                     LDNATRSWRSDNPALSPYHLVLGDGPESSAESAAPSPWAVPRLDVRDRVPPVDIAVVN
                     PHGFQRSRTAGTATVTVASEYTRFADARVEIAVGDTRLVTLTGHHQLSQRDLAGLRRL
                     RGVDVSGLSDASPVLGARVITQLAAAAVPVLGTIGPGTQGLLHPELMTALGASTITHL
                     DDDLAREAHAITARRAALRHHSIDAAWRALRVELGHDARPEPPVSVLLVTKRRSFLSH
                     ALAQAAAQDYPNLEIVLGLHGVDISDEELAPLLAGVRQPVVVVRRPSTTVYGDLLAEV
                     SRHASGELIAKMDDDDLYGPHHVTDLAQALRWSQATLVGTLVEWVYLAGADRTIYRPV
                     RSSPEAFGQWVGGGTLMLRADDLRALGG"
BASE COUNT         1563 a         4594 c         4523 g         1819 t
ORIGIN      
        1 cggcgctcgc cgaggcgttg gcgcgttcgg acccgcgact gcgctacctg cggctgccga
       61 tcaactccgg ggcggctggg cggccccgca acgagggcat cgacgcgacg cgggcgccgt
      121 acgtcatgtt cctcgacagc gacgacgagc tggagccgca cgcctgcgcc gacctgctcc
      181 gggcggtgcg cgagaccgac gccgagctgg cctcggggct gtgcgtgcgg gtgctcgtcc
      241 aggacgggca cgccgagacg ccgtggtatc cccggctcta ccgcgagcgg ctgacctatg
      301 acgacgtcgc cgagcggccc ggcctgctgt tcgacaccaa ctccaccaac aagatctacc
      361 ggcgctcctt cctcgagcgc accggcctgc gctaccccga gggccgcacc tacgaggacc
      421 tgctgttcac cacggcggcc tactgcctgg cccggcgcat caccctgacg ccggagcggg
      481 tctaccggtg gtacgtcgac gagcgcgccg accggctgtc ggtgaccaac cggcgcgccg
      541 agctggccaa cctggcggcg cgcctgtcga tccatcgcga catcgacgcc tggctgctcg
      601 cgcacggccg ggcggcgctg aaggtccaca aggacgtcaa gttcctccag cacgacctgc
      661 gcctctacct gggtgacctg ccacggcgcg accccgccta tcagcaggag ttcctcgatc
      721 aggccgggga ctacctcgcc gggctcaccc ccgaggggct cgacctcgcc ggcacgatgc
      781 tgcgggtggc cgcctacctg gtgcgcgcgc acgacctcga cggagtgctg gcggtctccg
      841 cctggctggg ccagcgtcag cggctgacca tcgacctggt cgccgacggc gaccaggtgt
      901 tctggggcga gcgccacctc gacacccccg agggccggca cgccctcgac gtcaccgacc
      961 tcgggctggc cgaccggccc ctctcaaagc tgcacctgca gacgacggcc acctcggtgg
     1021 cggtggatgg ctcggccctg accgtgcgcg ggaggacccg caaccagctc gacaaggtcg
     1081 gtgccggctc ggccgagctg gccctgatcc tgaccgcccg cgggtcgcac cggcagtgcg
     1141 ccgtaccgat gtccgagatc tgccaccgcg gccggtcgat cgcgtggggc gccagcgtgc
     1201 cggtggccga cctgctgggc gtcatcgagg cggacgagcg ggtctgggac gtggccctgc
     1261 ggatccgggt gggtgaggag gttgcgctcg ccacggtgct ggtggatggc gtggcgctac
     1321 cgggcggtct gcccgtgcgg gtgcggcggc ggttgctggc cggcgacacg ctggtgccct
     1381 atgccaccgt caacggcaac ctggcgctgc ggctggtggc gagccgaccc agcacccgcg
     1441 tggtccgtgc cctcacccag cgactcctcc ataccttcgc tcgtcgtcca cgccgccgcg
     1501 gatgatcgtg cgggcgggtc ggcgcggcga cctaggatga cggcggaatc cgcgcaaggc
     1561 cgatgacatc aacgaaggag gccgtcctgg tcagggtgct ggtcatcggc gaagccggac
     1621 ttgaccaggt ccgcgagcag ctggccacgc tcggcctacg agacgccacg gcggtctccc
     1681 ccgctgccgt cgaccagctc accgcgctga ccggcaccgg cgagccggtg ctgctcgtcg
     1741 acgccggctg ggtggggcac actcacgcgc tgcgactggt cggcaccgac ccacggcccg
     1801 gcgtgagggt tgccgcgggc ctgctggccg ccagccccga ccacctcgac gacctgctcg
     1861 ccgccgcgcg tcaggccggc cggctgaccc ccgatgcgct cgccgacacc ctgcgggccg
     1921 accacgtcac gctcatcgag ccggtcaacg gcagtctcgt ctcggcagtg gccaccgacc
     1981 ccgcccatcg cgccgccctg gccgacgagc gagacggcgt cgacgaggag gcgctgcggc
     2041 tggccggcgc ggtcaaggcc cacgacggct tcttcacgac ccacttcgtc agcccctact
     2101 cgcgctacct ggcgcgctgg ttcgcccgtc taggttggcg gcccaaccac gtcacgatgc
     2161 tgtcgatgct ggtcgccctg ctggcggcgg cgctgtgcgc cacggggcag cggtggggct
     2221 acgtggccgg cgcggtgctg ctgcaggcct cgttcacgct cgactgcgtc gacggccagc
     2281 tggcccgcta cacgctcgac ttctcccggg tcggcgcgtg gctggacgcg acgttcgacc
     2341 gggccaagga atacgcgctg ttcgccgggc tggcgatcgg ctcggcccgc accgggacag
     2401 acgtgtggct gctggccggg gcggcgatgt cgctgcagac cgtgcgccac agcctcgact
     2461 tcgcctttgc ggccgcgagc gacccgaccg cacgctcgac gtcggcggcc cggctgtcgt
     2521 cacgactgga cgcggtcggc tggacggtgt gggcccggcg catggtcatc ttgccgatcg
     2581 gcgagcgctg ggcgctgatc tcggtgctcg tcgcgctgac ctcaccgcgc accacgttcg
     2641 tcgtcctgct cgtcgccggg acgctggccg gcgcctactc gctgggtggg cgggtgctgc
     2701 gctcccgcgg cctcgccacg agcccgcagg cggccagccg gttgcgcgcg atggccgacg
     2761 gtggcgagcc ggcccggctc tcggcggcga cccggctcgg cacgctgccg gcaagcccgc
     2821 tggccctggc cggtgcgctg gcgctggtgg cggggctcgt cgccaccggg accgcaggcg
     2881 atgccggcgt ggtgctgctc gtcggcgcgc tcggctacgt cgtactcgcc gggtcaggct
     2941 cggccggtcg gttcgccggc ccgctgggtt ggttggtggt cccactcgtg cgggccagcg
     3001 agtacggcgc caccctggcg ctgaccgccg tgatcgcccc cgaggcgctg cctgcggcgt
     3061 tcggcctgct cgccgccgtc gcctaccacc actacgacac cgtgtaccgt ctgcgaggtg
     3121 acgcggcgcc tccgccgcgc ctcgtggtcg ccctcagcgg cgggcacgac ggccgcgtcc
     3181 tgctcctgtg cgcgctcgcg ctcgccgggg cggcgacgct gcgcccaggc ctcgtcgtgg
     3241 tcgcggtttt catcgccgcg gtcgtgacca tcgaaagcgt tcggacctgg acccggcccg
     3301 acaccgcccg ggccggcagg cccctcgacg acgcaggaga cctcgcatga tcgggctggt
     3361 cctcgcggcc ggtgccggac gccggttgcg cccctacacc gacacgctgc ccaagacgct
     3421 ggtgccgatc gacgacgaga ccaccgtgct ggacatcgcg ttggccaact tcgccgagat
     3481 cgggctgacc gacgtcgcgg tggtggtcgg ctacgcggca gacgtcgtac gtgaccgcca
     3541 agccgcgctg gagaagcgtc acggtgtcac gctcgagctg gtgcacaacc ccaaggccga
     3601 ggagtggaac aacgcctact cgctgtggtg cgcgcgcgac ctgctcgggc agggcgtgct
     3661 gctggccaac ggcgacacgg tgcacccggt ctcggtcgaa cgcaccctgc tcgatgcgcg
     3721 cggcccggcg ctgctgctgg cgctcgacac ggtcaagtcg ctggccgacg aggagatgaa
     3781 ggtgcagtgg tcgcccgagc aggggctgac ccggatcacc aagctgatgg acccggcgac
     3841 ggcctacggc gagtacatcg gcgtcacgct gatcgagccc gatgccgccg cgccgctgac
     3901 cgaggcgctc gaggcgacct ggcggcgcga ccccaacctc tactacgagg atggctacca
     3961 ggagctggtc gaccgcggct tccgggtcga cgtcgcgccg atcgggacgg tcgactgggt
     4021 cgagatcgac aaccacgccg acctggctcg cgctcgggag atcgtgtgcc gctcctgacc
     4081 cggatggtga cggccccgct catcgtcgac gtacgacgcg gggcgttcga cgagctcgcc
     4141 acggtgcttg ccgaccagcg catctccacc gccgggcggg tcgccatcgt cctgggtccg
     4201 cactcggggc aacggctgcg tcctcggttg gagcaccagc ggctgcacgc cgactacttc
     4261 accgtctcgg ccggcaccgt cgacgccgcg gtggacctgg tcgaggcggc ccggcgcagc
     4321 acctacgacg cgatcgtggg catcggcggc ggccgggtgc tcgacgtcac caagttcgcc
     4381 gcggcccggc tcgggctgcc gatggtcgcg gtggccacca acctcgcgca cgacggcatc
     4441 ggctcgccgg tcgccatcct cgacaacgac cgggggcgcg gctcctacgg cgtacccgcg
     4501 ccgatcgcgg tgctcgtcga cctagacctg gtccgccagg cgccgcgggc ctcggtcgcc
     4561 gcgggcatcg gcgaggcgct gtccaacctg tcggcggtgg ccgactggga gctggcccac
     4621 gaggtcaccg gcgaggcgat cgacgggctg gccgtgacga tgtcgcgcac cgccgcccgc
     4681 tcgctgctgc acgacccggg cgacctcgac tccgacgcgt tcgtggccct gctggccgag
     4741 gcgctggtcc tctcgggcct ggccatggtc gtcgccggca cctcgcgccc gtgcagcggc
     4801 gcctgtcacg agatctcgca cgccatcgac ctgctgcacc ccgaccgggc cggcgcgcac
     4861 ggcgagcaag tcggggtcgg cgccgcgttc gccacctggc tgcgcggtga ccatgagctg
     4921 tccgccacga tcgtccaggt gctgcggcgg cacggtctgc cggtcggccc ggccgacctc
     4981 gggttcaccg atgcagagtt cgtcgagatc ctcgcgttcg cgccgcagac ccgccctggc
     5041 cgctacacga tcctcgagca cctcgacctg gatgcggccg ggatcgccga cgctgtgagg
     5101 ggttatgcca gcgccatcgg ttgctgagct ccgccgggtg gcccagcccg agggcgtgct
     5161 gggccggcgc agcggagagc actgggccgg ccggctctac atgcgccacg tgtcgatcta
     5221 tgccacccgg gtgctcgtcg cgctgcgggt ggcgcccaac acggtgaccg ccgggatgat
     5281 cgtcatcggg ctgctggccg ggccggcgct gctgctgccc ggcgtgtggg gggcgttgct
     5341 ggccgcgctg ctcgtgcagc tctacctgct gctcgactgc agcgacggcg aggtggcccg
     5401 ctggaccggt cgtacgtcga tcagcggggt ctacctcgac cgggtggggc actacctgtg
     5461 cgaggccgcg ttcatggtgg gtctaggcct gcgggccgac gaccttgagg tcggcggcta
     5521 cgcgatgctc ggcacgctga ccgcgctggg cgtcgtgctc atcaaggccg agaccgacct
     5581 cgtcgacgtc gcccgggccc gggccgggct gccggcggcc gaggacgcgt cggccgcgcc
     5641 acggtcctcc ggcgtggcgc tggcccgccg gctggcggcc ggactgcgcc tgcaccggct
     5701 gctcggcgcc gtggaggcct ccctgctggt gctcgcggtt gcggtggtcg acgcggtgcg
     5761 cggtgacctg cgcgccaccc aggtcaccgt ggtggtgctg gtggtggtgg ccggcctgca
     5821 gctggtggcg cacctggtga gcatcctggc gtcgcggcgg ctggcgtgag ccccaccgtg
     5881 ccgacactgt cgtgcgtgct gctgacgatg ggcgaccggc ccgccgagct cgcgcgtgcg
     5941 gtgcacagcg tgaccagtca gcacggcgcg ccgatcgagg tggtcgtggt cggcaacggc
     6001 gtcgcggtcc cggctcctgc ccccgccgta cgcacgatgt cgttgccgga gaacgtcggg
     6061 gtgccggccg ggcgcaacgt gggttgggcg cagacctcgg ccgacgtggt gatcttcctc
     6121 gacgatgacg ggtggttccc cgagcccgac ctgggtgagc ggctgcgggc cgcgttcgcc
     6181 gccgacccgc ggctgggcat cgtggcgttt cgggtcaccg accccgacga cgccagcacc
     6241 cagcgtcggc acgtgccccg gttggtggtc ggcgacccga tgcgctcctc cgaggtgacg
     6301 acgttcgtcg gcggtgcctg cgccgtacga cgcaaggtgc tcgaggaggt cggcgggctg
     6361 cccgacgcgt tcttctacgc ccacgaggag accgatctgg cctggcgcgc cctcgacgcc
     6421 ggctggcgga tccgctacga cgccggggcc ctcatgcacc acccggggac ctcgccgacg
     6481 cggcacgcgt cttacctgcg gctgaacgcg cgcaaccggg tctggctggc tcgacgcaac
     6541 ctgcccgccg cactggcggt gctctacctg ctgtgctggg tggcgctgac ggtggtccgg
     6601 gtccgccagc cggcggcgct gaaggcgtgg ttcgccgggt tcgtcgaagg cctgcgcacc
     6661 gatgccggac cccgccgtcc gctgcgctgg gcgactgctt ggcgcatgac tcgcctcggt
     6721 cgcccaccgc tgctgtgacc gaaatccgtg aagagccatg ccgtaggctc gcagccatga
     6781 ccgaccccgc tcgacaggtc tatgcggatg gggccgcaaa ggtggcgacc ggggcggacg
     6841 actcatctgg gggcccagcc gcgccctcgg ccgccgaggt cgcggcccac cacggcttgc
     6901 gccgcagcgc cgcgcggttg ccgctgtccg actacgtgcg cgagctgtgg gcacgccggc
     6961 acttcatcgt ggccttcgcc agcgcgcgca acatcgcggt ctacaccaac gcccggctgg
     7021 ggcaggtgtg gcaggtgctc accccgctgc tcaacgcggc ggtctactac ctggtcttcg
     7081 ggctgatcat caagacccgc ggcgggatcc ccaactacat cgcgttcctc gtcatcggca
     7141 tcttcatctt cacctacacg cagcggtcgg tgctcagcgg ctccaaggcc atcaacggca
     7201 acctcgacct gatccgggcg ctgcacttcc cgcgggccac cctgccgttc gcttacacca
     7261 tcatcgagct gcagcagctg gccatctcga tggtggtgat gctcgcgatc gtcctggcta
     7321 ccggtgagcc ggtcaccctg gcctggctgc tcatcgcgcc cgcgctgctg ctgcagacgc
     7381 tgttcaacat cggcgcgtcg ctgttcgtcg cccggctggc cgccaaggtc accgacgtga
     7441 cccagctgct gccgttcatc ctgcgcacct ggctctacgt cagcggggtg ttctactcga
     7501 tcgcgttctt caccgagagc gcgcccaagt gggtgcagac gatcctgcag gccaaccccg
     7561 gcgcggtcta catcgagatc atccgcaagg tgctgatcca ggaccacgag accgccgggc
     7621 cgatctgggc ctacgccgtg gtctgggccg tcgtggcctt cgtcggtggg ttcctctact
     7681 tctaccgggc cgaggagacc tacggccgtg ggtgagcacg ccatcccgac ggtggtcgtc
     7741 gacgggctgc acgtcgtcta ccgggtctac ggcgccagca gccgcaaggg caacgcgacc
     7801 accgcgctgc ggcgcatcct gcgccgtcag ggtgccccgg ccatgcgcga ggtgcacgcc
     7861 atccgtggga tctccttcac cgccaaccag ggcgacgcca tcgggctcgt cgggcgcaac
     7921 gggtcgggca agtccacgct gctgcgcgcg atcgccggcc tgctgccagc cgaccggggt
     7981 gcggtctaca ccgagggccg cccggccctg ctgggcgtca acgcggcgct gatgaacgac
     8041 ctgaccggcg agcgcaacgt cgagctgggc tgcctggcga tgggcatgag cccggcgcag
     8101 gtccgctcgg cgcacgacga catcgtggag ttcgccggca tcggcgactt catctcgctg
     8161 ccgatgcgca cctactcctc tggcatgggc gcccggctgc gcttcgcgat cgcggcctcg
     8221 cagacccacg acgtgctgct catcgacgag gcgctggcga ccggtgacgc ggagttccgc
     8281 gtcaagagcg agaagcgcat ccgtgagctg cgcgaggccg ccggcacggt gttcctggtc
     8341 agccactcgc tgtcggtggt gcgccagacc tgcaaccggg cgatctggct cgacgccggc
     8401 gagatcgtca tggacggccc cgtcgacgag gtgctgcacg cctacgaggc caagttcgac
     8461 cccaacgccc gaccggccag ccgcccgcgc gcggtctggt ccgccccgac cctgaaggag
     8521 tggttggcca tggccaccga ggacgacctg cgccggatcg tgcgcgagga gctggtcgcg
     8581 ctgctgggtc gcgacgactc cccgctcgcg gtggagaacg acgacttcga cccgggcacg
     8641 cccggcggag ccaaccgcaa gatctcgctg accagggcga tctaccgcgc ctactcgcag
     8701 tcgcgcaagg cggtcggctc gctcacccgt ccgagctcga ccgactgacc ccaccggcca
     8761 tcgcgagcag cccggcgtag agacgcgcgg cgcgggcgcc ccggctctct ctgcttcgcg
     8821 ccgacgcgac cgcgtcgcgg tgccagcgcc acagggtcgg atcggcatag agcgcaggca
     8881 gtacgtcggt cagctcggcg ggactgcgta cggcgggcgc gccggctgtg cccgtgccgg
     8941 ccacgtcgat cgcgagccgg tgggtcggct cggccgccgg ggcgacagcg gtcagccgat
     9001 cgtccagcgc cgcaaccgca tctgggtccc cgaccaccag cagcggagct ccaggcagca
     9061 acgcctgcag cgcctgcacc cactcgctca ccggctcggc caacgcctgg ctgttggcca
     9121 gggtcgccgc gtgaccgagc aggaccccag ccgtctgccc gaaggccagc gggcgaggcg
     9181 ccgcaccggt gccgggcgcg tcgtcgaccc gcaaagcccg cccgcccggg tcgaggacga
     9241 acccacccag ggaccgggtc acgtcgagcg tcaccgtgcc cgccgcgtcc tcggcgtcga
     9301 cccggacccg caagccatac gcgccctgcg cgtcgtctcg gtcgacgtcg acacccaggg
     9361 gcgccaacgc gtggcggagc gcggcctcga gcgcgtcggc gtccgcctgg tcccgcgccg
     9421 ttgacggcgc cacgctcagg cgcaacggag gccgagtcag gagcgcgggc gtcgtacgga
     9481 tctgtgaacc ggccaggacc ggcaggtgct cacgcaccgt gtcccgggtg tcgaaccggc
     9541 tcgcgttggc gtcggtggag gcgccccaga tggtcgccgc gcgggggaac gggattccgg
     9601 cggcccagcc ggcgcggtgg gccagcgtga cgaggggcac gtcgtgtcgt aaggcctgca
     9661 gcgccatgaa gatgtcggcc atgttgggcg ccgggaagtc ggccagcgtc agcgccagcg
     9721 tgtcggtgtg cagggcggca gcaccggtgc cgagcacgtg gacgaaggtg tccttggcca
     9781 cgtccttgct gaactcgagc actcgtcggc ggctgtggtc gtagaagtcg gtgatcgcgg
     9841 ggtcgaggat cgagccgtgc cagccgacga tggcccggcg gccgtagcgc tcgatgccgt
     9901 cgatgaggtg ctcgacgtag aacgacgggt aggtgatgtc gtcgtcgacg gtgacgtagt
     9961 agccgctgaa cccgtcgatg aagcggaact tgccgcggtc gccaaggtcc acgcccgaga
    10021 cggcgcgcag cttggcgtcg tcgcgcagga agctcgggat ggcgtcgtag ccgttgaggt
    10081 gcacgcagat ctcgtcggcc tggtcgtaga gcgcctcgac gaccttggcc agtccggcct
    10141 cgcgttgcgg gatcgaggcg atgccgaccc ggatgcgctc tcggccactg cccgatcgcc
    10201 ttgaggccgg gggcaggtcg tcgccgtccg gccgacgggc cggcgcgcct agagccttgg
    10261 cgatggcccg ggcgcgggcc tcggtgtcga gcccggtgcg ggcacccgac ccgatgcctg
    10321 cacgggccgc cagggcacgc agccgttggg cggtctgcac cgggcgggac cgggccagcc
    10381 ggtcgtagga ctcgcgccag agcgagacct cacgctggag ctgggtcagc gcgtcctcga
    10441 aaccaggcga gctctgggac acgggtcgca actgtagccg cgaggctgcc cggccggtgc
    10501 cgcgccgggg accgcgcggg cccgctgact atgctcgcgg caatgtcgcc gctgctgcgc
    10561 aacccgcgct tctacgcgtt ggtcgccgtg atcacgctgg ccaccgtggc cgccgccgta
    10621 ctcatcaatc ccaccgcggc cgccgcggtc gtggctgccg tcgtcggcgt cacgctcgca
    10681 gtgacgctgc gggcgttgcg gctgagccaa cagcaggccg ccgcgacccg cgtcgcgtcc
    10741 gccaaggccg ccgcggccat gcagaccgac ctgggccggc tgcgggccga gctcgacacc
    10801 gtgcacgacg acgtgacgca gctgctgctc cggccggtcc cggagcggag cgaggcgaac
    10861 gacgggctgc tcgtcaccat cggtgccgcc cgcgcggccg cggtcagcgc gacgctggcc
    10921 gagctgccgg tcggcacccg cgtcgtggac ctgctcgggt gctcgccggc cgaggtggac
    10981 ggcctgcacg ccgacgaccg cgccgcgctc gctctggtgg ccgacagtgt cgccggcctg
    11041 cgtctggctt tgccggcctt ggagtcgctg gggtccgcgc gccggctggt gatcgccatc
    11101 gacgacgtgg cagcgcgccg gggcgccgtc ctcgccgccg ccaatccggg cctgctggct
    11161 cccacctcgc tgcagcacgc ggctgcagcg ggcggtggtt cggtggtccg ggtcggcttt
    11221 gccgcgccgg tgcgcgtgca ccgcgtcgcg gccacgctgc tgactggctg ggcctcggga
    11281 cggcggcgca tcgacgccgc gggcctgcgc gtcgcggtca tgtcgtgggc ggcgctgccc
    11341 tgggtggccg gtgacccgag tgcccgcact ctcgacaacg ccacgcggtc gtggcgctcg
    11401 gacaaccccg cgctctcgcc ctaccacctg gtgctgggtg acgggccgga gtccagcgcc
    11461 gaaagcgcgg ccccgtcgcc gtgggcggtc ccgcggctgg acgtgcgcga ccgggtgcca
    11521 ccagtcgaca tcgcggtggt caatccccat ggtttccaac ggtctcggac cgctggcacg
    11581 gccaccgtga ccgtggcctc ggagtacacg aggttcgcgg acgctcgggt ggagatcgcc
    11641 gtcggcgaca cccgcctggt cacgctcacc ggccaccacc agctgtccca gcgtgacctc
    11701 gcggggctgc ggcgactgcg cggtgtcgac gtcagcggcc tgtccgacgc gtcgccggtg
    11761 ctgggcgcac gggtgatcac gcagctggcg gcagccgccg taccggtgct cggcaccatc
    11821 ggccccggca cccagggcct gctgcacccg gagcttatga cggcgctggg cgcgtcgacg
    11881 atcacccacc tcgacgacga cctggctcgg gaggcgcacg cgatcaccgc gcgtcgggcc
    11941 gcactgcgtc accacagcat cgacgcggcc tggcgcgcgc tgcgcgtgga gctcggtcac
    12001 gacgcccggc cggaaccgcc ggtgtcggtg ctgctggtca ccaagcggcg ttcgttcctc
    12061 tcccacgcgc tggcccaggc ggcggctcag gactacccca acctcgagat cgtcctggga
    12121 ttgcacggtg tggacatctc cgacgaggag ctcgcgcccc tgctggccgg cgtacgccaa
    12181 cccgtggtcg tcgtacggcg accctccacg accgtgtacg gcgacctgct cgcggaggtc
    12241 agccgtcacg cgtccggcga gctgatcgcc aagatggacg acgacgacct ctacggaccg
    12301 caccacgtga ccgacctggc ccaggcgctg cgctggtcgc aggcgaccct cgtcggaacc
    12361 ctggtcgagt gggtctacct ggccggggcc gaccgcacga tctaccggcc ggtgcgctcg
    12421 agcccggagg cgttcgggca gtgggtcggt ggcggcacgc tgatgctgcg cgccgacgac
    12481 ctgcgcgcgc tgggcggct
//
LOCUS       DATIBB010000059         4923 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_423497, whole genome shotgun sequence.
ACCESSION   DATIBB010000059 DATIBB010000000
VERSION     DATIBB010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 4923)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4923)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4923
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_423497"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            5..949
                     /locus_tag="VLC50_02180"
     CDS             5..949
                     /locus_tag="VLC50_02180"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020389149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HSF26304.1"
                     /translation="MGLRKAMEDDPKVLIMGEDVGKLGGVFRVTDGLQKDFGEDRVID
                     TPLAESGIIGTAIGLALRGYRPVCEIQFDGFVFPAYDQIISQLAKVHFRSLGRLPMPV
                     VVRIPFGGGIGAVEHHSESPETLFAHIMGLKVVACSNPVDAYWMIQQAIRTDDPVIFF
                     EPKRRYWEKAELDESVPPALPLHAAHVLREGTDLTLLCWGPMVKTCLQAATAAEAEGR
                     SIEVLDLRSIAPYDLPAIYASVRKTGRVVVVHESPLSFGPGAELAARVSEACFYSLEA
                     PVMRVAGFDTPYPPAKIEEDYLPDLDRVLDSVDRALAF"
     gene            986..2410
                     /locus_tag="VLC50_02185"
     CDS             986..2410
                     /locus_tag="VLC50_02185"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020577848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HSF26305.1"
                     /translation="MSETRQFTLPDVGEGLTEAEIVSWKVKPGDAVKVNDVIVEIETA
                     KSLVELPCPWAGTISELLVAEGQTVEVGAPIVAVAIGEGASAGSAGSAASAPSEPVQA
                     ATEIGEPKPDQAVEPGLIGGPAPGGRTSVLVGYGPRTTEAKRRPRAGAHAAAPTPTVI
                     KQSVPAPAAPAPAPAAPAAAGPVLAKPPVRKLAKDHGIDLRTVTPTGAGGIVTRADVE
                     AAVEGATEPSYDVSGLGRPAAAGERETRIPIKSVRKVMAEAMVRSAFTAPHVTEWVSV
                     DVTRMMALVKRLRALPEFAEVRVGPLTLVARALILAVRRNPLVNASWDEAAGEVVVKN
                     YVNLGIAAATPRGLVVPNIKDADRLTLAELATALSDLAHTARAGKTPPADMLGGTISI
                     TNVGVFGVDSGTPILPPGESAILAFGQVRELPWVHRGKVKPRQVTQLALSFDHRLIDG
                     ELGSKFLADIARLLEDPGLALAYA"
     gene            complement(2504..3349)
                     /locus_tag="VLC50_02190"
     CDS             complement(2504..3349)
                     /locus_tag="VLC50_02190"
                     /inference="COORDINATES: protein motif:HMM:TIGR03083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase family
                     mycothiol-dependent enzyme"
                     /protein_id="HSF26306.1"
                     /translation="MTTTDDSPTPPTPTAPPLTPYVDAYEQTMRATIALGHELSDEDW
                     DRPTECPGWTVRDQYAHLVGVERAVLADPRPEHPVPDLPHMRNEFAQFTELDVDYRRD
                     RSGPTVLAELEETLDRRLAVLRDPTLTEESMLLGPFGMRPGRLTLGVRVFDIWAHGED
                     VRRATGRPADLDSAAAGVAVRWIRRALAKVIGTDTGLPEGSTVVIEVVGPQGFTEAVR
                     VGAEGRGERLSEVPADQTVHLSMGTEAFTRRACGRWSVEATPVQLMGDTEAGQAVLTA
                     LTITP"
     gene            3368..4141
                     /locus_tag="VLC50_02195"
     CDS             3368..4141
                     /locus_tag="VLC50_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015659112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen family hydrolase"
                     /protein_id="HSF26307.1"
                     /translation="MRISVLQVAYDDVEPLPARVERVAGLVRSDGAGADLVVLPELWG
                     PGGFSYDRWATGAEPMDGPIVTAMREAAAAIGAHVHMGSLVERDGDRLYNTSVLLDPA
                     GEIVATYRKIHLFGFGEGEPVLMSPGEAVVTAKVGDTTVGLATCYDLRFPELFRRLID
                     DGAEVIVVPAAWPARRLAHWSLLARARAVEDQVFLVACNTSGTHAGHLMGGHSAVIDP
                     WGEALAEAGEGAESLVVDVDPAFVAVTREKFPVLRDRRL"
     gene            complement(4429..>4923)
                     /locus_tag="VLC50_02200"
     CDS             complement(4429..>4923)
                     /locus_tag="VLC50_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011461272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-eliminating lyase-related protein"
                     /protein_id="HSF26308.1"
                     /translation="LAGRDMEAMAQGIHEMVDDDYIAHRVHQVRHLGETLLGAGVPIV
                     EPIGGHAVFLDARRILGHLDQEALPAQALAAALYVDSGVRAMERGIVSAGRDPATGRN
                     RAPRLELVRLTIPRRVYTDRHLDVVAESVVRLHERREEITGLRWVYEPPTLRFFTARF
                     APLP"
BASE COUNT          660 a         1783 c         1773 g          707 t
ORIGIN      
        1 caccatgggc ctgcgcaagg ccatggagga cgaccccaag gtcctgatca tgggtgagga
       61 cgtcggcaag ctcggcggcg tcttccgggt caccgacggg ctgcagaagg acttcggcga
      121 ggaccgggtg atcgacaccc ccctggccga gtcggggatc atcggtacgg cgatcgggct
      181 ggcgctgcgc ggctaccggc cggtgtgcga gatccagttc gacgggttcg tcttcccggc
      241 ctacgaccag atcatcagcc agctggccaa ggtgcacttc cgttcgctgg gccggctgcc
      301 gatgccggtc gtcgtacgca tcccgttcgg tggcggcatc ggcgcggtcg agcaccactc
      361 cgagtcgccc gagacgctgt tcgcccacat catggggctc aaggtggtgg cctgctccaa
      421 cccggtcgac gcctactgga tgatccagca ggcgatccgc accgacgacc cggtgatctt
      481 cttcgagccc aagcggcgct actgggagaa ggccgagctc gacgagtcgg tgccgccggc
      541 gctgccactg cacgcggccc acgtgctgcg cgagggcacc gacctgacgc tgctgtgctg
      601 gggcccgatg gtcaagacct gcttgcaggc cgcgaccgcc gccgaggccg agggccgctc
      661 gatcgaggtg ctcgaccttc gctcgatcgc gccctacgac ctgccggcca tctacgcctc
      721 ggtgcgcaag accggccggg tcgtggtcgt gcacgagtcg ccgctgtcct tcggcccggg
      781 cgccgagctg gccgcccgcg tcagcgaggc gtgcttctac tcgctggagg cgccggtgat
      841 gcgggtcgca ggcttcgaca cgccctaccc gccggccaag atcgaggagg actacctgcc
      901 cgacctcgac cgggtgctcg acagcgtcga ccgcgcgctg gccttctaag gccggcgcag
      961 cctttctgag gaggagttcg cacccgtgag cgagaccagg cagttcacgc tccccgacgt
     1021 cggcgagggc ctgaccgagg ccgagatcgt ctcgtggaag gtcaagccgg gcgacgcggt
     1081 caaggtcaac gacgtcatcg tcgagatcga gaccgccaag tcgctggtcg agctgccctg
     1141 cccgtgggcc ggcacgatca gcgagctgct ggtcgccgag ggccagaccg tcgaggtcgg
     1201 tgccccgatc gtcgcagtcg ccatcggcga gggcgcgtcc gcgggatcgg cggggtcggc
     1261 cgcctcggcc ccctccgagc cggtgcaggc cgccaccgag atcggcgagc ccaagcccga
     1321 ccaggcggtc gagccggggc tgatcggcgg gccggccccg ggcgggcgca cctcggtgct
     1381 ggtcggctac ggcccgcgga ccaccgaggc caagcgacgc ccccgtgccg gcgcccacgc
     1441 cgcggccccc acccccaccg tgatcaagca atccgtgccc gcccccgcgg cccccgcccc
     1501 ggcacccgcc gcccccgcgg ccgctggccc ggtgctggcc aagccgccgg tgcgcaagct
     1561 ggccaaggat cacggcatcg acctgcgcac cgtcacgccc accggcgccg gcggcatcgt
     1621 gacccgcgct gacgtggagg ccgctgtcga gggtgcgacg gagccgtcgt acgacgtgag
     1681 cggcctgggc cgtcccgccg cggcggggga gcgggagacc cgcatcccga tcaagtcggt
     1741 gcgcaaggtg atggccgagg cgatggtgcg cagcgcgttc accgccccgc acgtgaccga
     1801 gtgggtcagc gtcgacgtga cccggatgat ggcgctggtc aagcggctgc gcgcgttgcc
     1861 ggagttcgcc gaggtgcggg tgggcccgct gaccctggtc gcgcgcgcgc tgatcctcgc
     1921 cgtacgccgc aatccgctgg tcaacgcctc ctgggacgag gcggccggcg aggtcgtcgt
     1981 gaagaactac gtcaacctcg gcatcgccgc ggccaccccg cgcgggctgg tcgtgcccaa
     2041 catcaaggac gccgaccggc tcacgctggc cgagctggcc accgcgctct ctgacctggc
     2101 gcacaccgcg cgcgccggga agacgccgcc ggccgacatg ctcggcggga ccatctcgat
     2161 caccaacgtc ggtgtgttcg gcgtcgactc gggcaccccg atcctgccgc cgggcgagtc
     2221 ggcgatcctg gcgttcgggc aggtgcgcga gctgccctgg gtgcaccggg gcaaggtcaa
     2281 gccgcggcag gtgacccagc tggcgctctc gttcgaccac cggctcatcg acggtgagct
     2341 gggctcgaag ttcctcgccg acatcgcccg cctgctcgag gacccgggcc tcgccctcgc
     2401 ctacgcctga gccgcctggc tcggcaggac gtcgacgttg gcgacggaca cgccggcgtg
     2461 tcgcgtcggg aacgtcgacg tcttggtgat ggggggtggc gggtcagggg gtgatcgtga
     2521 gggcggtcag caccgcctgc cctgcctcgg tgtcacccat gagctggacc ggggtggctt
     2581 ccaccgacca gcgcccgcag gcgcgccggg tgaacgcctc ggtgcccatc gacaggtgca
     2641 ccgtctggtc ggccggcacc tcgctcaacc gctcgccgcg gccctcggcg ccgacccgca
     2701 ccgcctcggt gaacccctgc ggcccgacca cctcgatcac cacggtcgat ccctcgggca
     2761 gcccggtgtc ggtgccgatc accttggcca gcgcccggcg gatccaccgc accgccacgc
     2821 cggcggcggc cgagtcgagg tcggccggtc gcccggtggc ccgtcgtacg tcctcgccgt
     2881 gtgcccagat gtcgaacacc cgcacgccca gcgtcagccg gcccgggcgc atcccgaacg
     2941 ggcccagcag catcgactcc tcggtcagcg tcgggtcgcg cagcaccgcc aggcgccggt
     3001 ccagcgtctc ctcgagctcg gccagcaccg tcgggccgga gcggtcgcgg cggtagtcga
     3061 cgtcgagctc ggtgaactgg gcgaactcgt tgcgcatgtg cggcaggtca ggcaccgggt
     3121 gctcgggccg cgggtcggcc agcaccgccc gctcgacccc gaccaggtgg gcgtactggt
     3181 cgcgcaccgt ccagcccggg cactcggtcg ggcggtccca gtcctcgtcg ctcagctcgt
     3241 ggccgagcgc gatggtggcg cgcatcgtct gctcataggc atcgacgtac ggcgtcagcg
     3301 ggggtgcggt cggcgtcggg ggcgtcggtg agtcgtcggt cgtggtcacg gtgagaggct
     3361 aggacccatg cggatctcgg tgctacaggt cgcttacgac gatgtcgaac ctctgccggc
     3421 gcgggtggag cgggtggccg ggctggtccg gtccgacggc gccggcgccg acctcgtcgt
     3481 actgccggag ctgtggggtc cgggcgggtt ctcctacgac cggtgggcca ccggcgctga
     3541 gccgatggac gggccgatcg tcaccgcgat gcgcgaggcc gccgcggcga tcggggcgca
     3601 cgtgcacatg ggttcgttgg tcgagcgtga cggcgaccgg ctttacaaca cctcggtcct
     3661 gctcgaccct gccggcgaga tcgtggcgac ctaccgcaag atccacctgt tcggattcgg
     3721 cgagggcgag ccggtgctga tgtcgccggg cgaggccgtg gtcaccgcca aggtcggcga
     3781 caccacggtc gggctggcca cctgctacga cctgcggttt cccgagctgt tccgccggct
     3841 catcgacgac ggcgccgagg tgatcgtcgt acccgccgcc tggccggccc gccgactcgc
     3901 gcactggagc ctgctggccc gggcccgcgc ggtcgaggac caggtgttcc tcgtcgcctg
     3961 caacacgtcg ggcacccacg ccggacacct gatgggcggg cacagcgcgg tcatcgaccc
     4021 gtggggcgag gcgctggccg aggccggcga gggcgccgag tcgctcgtcg tcgacgtgga
     4081 cccggcgttc gtcgcggtga cccgggagaa gttccccgtg ctgcgcgacc gccgcctctg
     4141 accccacctc cttcgtgatc aagcaatccg cgcccgcgaa ctgtcggtga tcaagcaact
     4201 cgtgcccgcc aaccgtcggt gatcaagcaa tccgtgcact tgggtcacgt caccccaccg
     4261 cggtgccggt gcgtgaactg gcattgatcg tgtcagtttc cagggctctg acctgtgcga
     4321 tcttccacgg tcttggcgca gcagcactcg gcgtacacgg attgcttgat cacgagccag
     4381 aagtgagcac ggattgcttg atcaccgaca ggggggcggg ggcgcgcgtc aggggagtgg
     4441 ggcgaagcgg gcggtgaaga agcgcagggt cggcggctcg tagacccagc gcagcccggt
     4501 gatctcctcg cgccgctcgt gcagccggac caccgactcg gccaccacgt cgaggtgtcg
     4561 gtcggtgtag acccgccgcg ggatggtcag ccgcaccagc tccagccgcg gcgcccggtt
     4621 gcgcccggtc gccgggtcgc gcccagccga gacgatcccg cgctccatcg cccgcacccc
     4681 ggagtcgacg tacagcgcgg ccgccaacgc ctgggccggc agcgcctcct ggtcgaggtg
     4741 gcccaggatc cggcgggcgt cgaggaagac cgcgtgcccg ccgatcggtt cgacgatcgg
     4801 tacgccggct ccgagcagcg tctcgccgag gtggcgcacc tggtgcaccc ggtgggcgat
     4861 gtagtcgtcg tcgaccatct cgtggatgcc ctgggccatc gcctccatgt cgcgcccggc
     4921 cag
//
LOCUS       DATIBB010000060         4523 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_1080964, whole genome shotgun sequence.
ACCESSION   DATIBB010000060 DATIBB010000000
VERSION     DATIBB010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 4523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4523
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1080964"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..989)
                     /locus_tag="VLC50_02205"
     CDS             complement(<1..989)
                     /locus_tag="VLC50_02205"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSF26309.1"
                     /translation="MPPFHRPGRSRLPRLARILLVGVGLDAIGHGLTLPFLVVYLHDV
                     RGMSLATVGLLAAVPPVVALTLLGPLGVLIDRLGPRRVQVAALLASSGAAFVLSQTHA
                     VPAAVVAQLLVGVGHAAFWPANQSLIAELVPSVDRQHYFGVAFTLLNAGIGVGGLVSG
                     LLVSVADPTTFEWIYVVDGLSFAVPLAILLGPLRHVGGPVVVIGSSSPAGERRSGRPR
                     SGGSYRDVLADRVFRRVLAVAFLASLVGYSQMEAGWTAFARTVAEASTQTMGMAFAVN
                     TAAIVIVQLVVLRRLEGRRRTRALMVMAGVWALSWLIVGSSGLVPGTLLAAVLL"
     gene            1056..2492
                     /gene="larE"
                     /locus_tag="VLC50_02210"
     CDS             1056..2492
                     /gene="larE"
                     /locus_tag="VLC50_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012923480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HSF26310.1"
                     /translation="MPDLVVGFDLDMTLVDSRPGIAATLDALAAESGVFIDSALVTTR
                     LGPPLALELANWYPHEQIPAMNERFRVLYPELAVAPSELIPGAVAAIEAVRRHGGRVV
                     VVTGKHGPNAELHLRHLGLEVDVLVGGLHGERKAEALLEHGVQVYVGDHPGDIAGARV
                     AQAVAVGVASGSHDVDALTAAGADVALPSLETFPGWLDEWVLARRLERLDARLRELGS
                     VAVAFSGGADSAFVLAAAARALGPDRVVAVTAVSPSLPQRELAAARAFVADLGVRHET
                     PSTDEMSREGYRANAGDRCFFCKAELLEVLAPMAERLGVAAVATGTNADDVRAGFRPG
                     IRAAAERGAVTPLLDAGFTKAQVREASRRWGLVTWDKPAAACLSSRVAFGVQITPQRL
                     ARVEAAEDALRDALADAGLEVRNLRVRDLGERARIEVDRGSADAVAADPRFVEVVREA
                     GFAEVTVDLRGFRSGSMNELLADPSRYR"
     gene            2585..2968
                     /locus_tag="VLC50_02215"
     CDS             2585..2968
                     /locus_tag="VLC50_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017556174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HSF26311.1"
                     /translation="MPTGKVKWYDVEKGFGFLTSDEGGDVFVHSSVLPAGVSALRPGT
                     RVEFGVVEGRRGTQALQVRLLDPLQSAVKGQRKSAEEMTVIVEDLIKLLDGVSNTLRR
                     GRYPDRSVGQKVGAVLRAVADDLEE"
     gene            complement(2896..4350)
                     /locus_tag="VLC50_02220"
     CDS             complement(2896..4350)
                     /locus_tag="VLC50_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015659631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSF26312.1"
                     /translation="MTPSPPELPPGLDPRRSGPSTRAGRAARASARAARATVRGTASA
                     AAGTAHRVRRATSSQGAGESGLGRLTELHGVAIGADALVTVGLAGTLFFAVPVGEARG
                     RVALYLLVTMAPFVLVAPVIGPLLDRLRHGRRYALATTCWVRALLAWIMSAAVAGETE
                     DALRLYPAAFGLLVMSKAYHVTRSAMVPRVLPAQISLVKANSRISMAGLITAAVAAPV
                     GAGLAWFGPAWTLRLAVIAYVAGGVLCVLLPREADSAAGEEPARISGEPEPDARPRLR
                     NVGPAVVASLRANAALRAFAGFLTLFLAFLLRAHPLGDLSAGLVIGLAAVGAALGSFV
                     GTSLGALRTSRAPEAITVVVLVVATAACALGAGFYALWTIILVAAGAGFSQSLGKLAL
                     DALIQREVPERWRTSAFARSETALALAWVLGGGIGIVVPLSGRWGLGVAAVLLLAMLV
                     ATLLAQRRARVYSSRSSATARSTAPTFWPTDRSG"
BASE COUNT          650 a         1747 c         1643 g          483 t
ORIGIN      
        1 agcagcacgg cggccagcag ggtgccgggc acaagccccg agctgccgac gatcaaccac
       61 gacagcgccc acaccccggc catcaccatc agggcccgcg tgcgtcggcg cccctcgagc
      121 cggcgcagca ccacgagctg cacgatcacg atcgcggcgg tgttgaccgc gaaggccatc
      181 cccatcgtct gcgtcgacgc ctcggcgacg gtgcgggcga acgccgtcca gccggcctcc
      241 atctggctgt agcccaccag cgaggccaag aacgccaccg ccagcactcg gcggaagacc
      301 cggtcggcga gtacgtcgcg atagctgccg ccggaccgcg ggcggcccga gcgtcgctcg
      361 cccgccggcg aggagctgcc gatgaccacc accggcccac cgacgtggcg cagcgggccg
      421 agcaggatgg ccagcggcac ggcaaagctc agcccgtcga cgacgtagat ccactcgaac
      481 gtcgtcgggt cggccacgct gaccagcagg ccgctgacga ggccgccgac gccgatgccg
      541 gcgttgagca acgtgaacgc caccccgaag tagtgctgac gatcgacgct gggcacgagc
      601 tcggcgatca gcgactggtt ggcaggccag aacgcggcat gccccacgcc gaccagcagc
      661 tgggccacca ccgcggcggg caccgcgtgg gtctgcgaca gcacgaaggc cgccccgctg
      721 gaggcgagca gcgcggcgac ctgcacccgg cgcgggccga gtcggtcgat gagcaccccg
      781 agcgggccga gcagcgtcag cgccaccacc ggcggcaccg ccgcgagcag ccccaccgtg
      841 gccagcgaca tgccgcgcac gtcgtgcagg tagacgacga gaaacggcag cgtgagcccg
      901 tgcccgatcg cgtcgagccc gacgccgacg agaaggattc gcgcgagtcg gggcagccgg
      961 gaccgacccg gacggtggaa ggggggcacg gccgcaagga ttccacccct cgccgacagg
     1021 actcatccgg ttttgtgccg actacgctgc ttggcgtgcc agacctcgtc gtcgggttcg
     1081 acctcgacat gaccctcgtc gactcgcgcc cgggcatcgc ggccacgctc gacgcactgg
     1141 ccgccgagtc cggggtgttc atcgactcgg cgctggtgac cacccggctc ggcccgccgc
     1201 tggccctcga gctggccaac tggtacccgc acgagcagat cccggcgatg aacgagcggt
     1261 tccgggtcct ctaccccgag ctcgcggtgg ctcccagcga gctgatcccc ggcgcggtcg
     1321 ccgcgatcga ggcggtacga cgtcacggcg ggcgcgtcgt cgtcgtcacc ggcaagcacg
     1381 gccccaacgc cgagctgcac ctgcgccacc tcggcctcga ggtcgacgtg ctggtcggcg
     1441 ggctgcacgg cgagcgcaag gccgaggcgc tgctcgagca cggcgtgcag gtctacgtcg
     1501 gcgaccaccc gggtgacatc gccggcgccc gcgtggccca ggccgtcgcc gtcggggtgg
     1561 cctccgggtc gcacgacgtc gacgcgctga ccgcggccgg ggccgacgtc gccttgccca
     1621 gcctcgagac cttccccggc tggctcgacg agtgggtgct ggcccgccgg ctcgagcggc
     1681 tggacgcgcg gctccgcgag ctcggctcgg tcgccgtggc cttctccggc ggggccgact
     1741 cggccttcgt gctggccgcc gccgcccgcg cgctcggccc cgaccgggtg gtcgcggtga
     1801 ccgcggtctc cccgtcgctg ccgcagcgcg agctggccgc cgcccgcgcg ttcgtcgccg
     1861 acctcggcgt acggcacgag accccgagca ccgacgagat gtcgcgtgag ggctaccgcg
     1921 ccaacgccgg cgaccggtgc ttcttctgca aggccgagct gctcgaggtg ctcgcgccga
     1981 tggccgagcg gctcggggtg gccgcggtcg cgaccggcac caacgccgac gacgtccgcg
     2041 ccgggttccg ccccggcatc cgggccgcgg ccgagcgcgg cgccgtcacc ccgctgctcg
     2101 acgccggctt caccaaggcc caggtccgcg aggccagccg ccggtggggt ctggtcacct
     2161 gggacaagcc agccgcggcg tgcctctcga gccgggtcgc cttcggcgtg cagatcaccc
     2221 cccagcggct ggcccgggtc gaggcggccg aggacgcgct gcgcgacgcg ctggccgacg
     2281 ccgggctgga ggtgcgcaac ctgcgggtgc gtgacctggg cgagcgggcc cgcatcgagg
     2341 tcgaccgcgg cagcgccgat gcggtggccg ccgacccccg gttcgtcgag gtggtgcgcg
     2401 aggccggctt cgccgaggtc accgtcgacc tgcgaggctt ccgctccggg tcgatgaacg
     2461 agctgctcgc cgatccatcc cgctatcgct gagccagcgt tagcctgggc gccggtcagc
     2521 aggtcggcac gacccgcgcc aacccgcacc aacccgctac cgacgacacg aacgaggtct
     2581 gcccgtgcct accggcaagg tcaagtggta cgacgtcgag aagggcttcg gcttcctcac
     2641 cagcgacgag gggggcgacg tcttcgtcca ctcgtcggtg ctgcccgccg gcgtcagcgc
     2701 gctgcggccg ggcacccggg tggagttcgg cgtggtcgag ggccggcggg gcacccaggc
     2761 gctgcaggtg cgcctgctcg acccgctgca gtcggcggtc aagggccagc gcaagtcggc
     2821 cgaggagatg acggtcatcg tcgaggacct catcaagctc ctcgacgggg tctccaacac
     2881 gctgcgccgc ggccgctacc ccgaccggtc ggtcggccag aaggtcggcg cggtgctgcg
     2941 cgcggtcgcc gacgacctcg aggagtagac ccgggcccgc cgctgggcga gcagcgtggc
     3001 gaccagcatg gccagcagga ggaccgcggc caccccgagc ccccaccgcc ccgacagcgg
     3061 tacgacgatc ccgatcccgc cgccgagcac ccaggccagc gcgagcgcgg tctcggaccg
     3121 ggcgaacgcc gaggtgcgcc accgctcggg cacctcgcgc tggatcaacg cgtcgagggc
     3181 cagcttgccc agcgactggg agaacccggc gccggcggcc accaggatga tcgtccacag
     3241 cgcgtagaac ccggcgccca gcgcgcaggc cgccgtggcc accacgagga cgacgacggt
     3301 gatcgcctcg ggggcacggg aggtgcgcag cgccccgagg ctggtgccga cgaagctgcc
     3361 cagggccgcc ccgaccgcgg ccaggccgat gaccagcccg gccgagaggt caccgagcgg
     3421 atgggcccgc agcaggaacg cgagaaacag cgtgaggaag ccggcgaagg cccgcagcgc
     3481 cgcgttggcc cgcaacgacg cgacgaccgc cggcccgacg ttgcgcagcc ggggccgggc
     3541 gtccggctcg ggctcgccgc tgatccgggc gggctcctcg cccgcggccg agtcggcctc
     3601 ccggggcagc agcacgcaca gcaccccacc cgcgacgtag gcgatcacgg caagccgcag
     3661 cgtccaggcc ggcccgaacc aggccagccc ggcgccgacc ggcgcggcca ccgcggcggt
     3721 gatgagcccg gccatcgaga tccgggagtt ggccttgacc aggctgatct gcgcgggcag
     3781 cacgcgcggc accatcgccg agcgggtgac gtgataggcc ttggacatca ccagcagccc
     3841 gaacgccgcc gggtagagcc gcagcgcgtc ctcggtctcc ccggccaccg ccgccgacat
     3901 gatccaggcg agcagggcac gcacccagca ggtggtggcc agcgcgtagc ggcgcccgtg
     3961 gcgcagccgg tcgagcagcg ggccgatgac cggggcgacg agcacgaacg gggccatcgt
     4021 caccagcagg tagagcgcga cccgcccgcg cgcctcgccg acgggcaccg cgaagaacag
     4081 cgtgccggcc agcccgaccg tgaccagcgc atcggccccg atggccaccc cgtgcagctc
     4141 ggtgagccgg cccagcccgc tctccccggc cccctgcgag ctggtcgccc gccggacccg
     4201 gtgggcggtg ccggcggccg cggacgccgt accccgcacg gtggcccggg cggcgcgggc
     4261 gctcgcgcgg gccgcccgcc cggcccgcgt cgacggcccc gagcggcgtg gatccaggcc
     4321 cgggggcagc tccggaggcg agggcgtcat cgcgaccatc ctgccgcgtg gtggtcaccc
     4381 cgaccacgat gggcgacaat caccgcgtgc ctgtcaccct tacggcccgc gctcgggccg
     4441 ccgccacaga cgccgtcctc acctctgccg ccgcgatcga gcttgctcgg accgccgcgt
     4501 atgacgaggc cggccccgac cag
//
LOCUS       DATIBB010000061         6517 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_160336, whole genome shotgun sequence.
ACCESSION   DATIBB010000061 DATIBB010000000
VERSION     DATIBB010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 6517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6517
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_160336"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..790)
                     /locus_tag="VLC50_02225"
     CDS             complement(<1..790)
                     /locus_tag="VLC50_02225"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006593475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HSF26313.1"
                     /translation="MNLHESPAATPTVPTSWRDRPAAQQPVWGDQAAVDEVTTSLRAL
                     PPLVFAGECDNLKAALGEVAAGRAFLLQGGDCAETFAANTADGIRNRIKTILQMAVVL
                     TYGASLPVVKLGRLAGQYAKPRSRDTEVRDGVELPSYRGDAVNDLAFTAAARQPDPRR
                     LLDVYHHSAATLNLLRAFTQGGFADLRQVHEWNRGFLNGSSAYHRYEVMARNIGRALA
                     FMQAAGADPESFRTVDLYASHEALLLDYEGALTRIDSRTGTPYDV"
     gene            complement(813..1838)
                     /locus_tag="VLC50_02230"
     CDS             complement(813..1838)
                     /locus_tag="VLC50_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017565081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HSF26314.1"
                     /translation="MRVGVLTGGGDCPGLNAVIRAIVRKGSTDYGYEVVGFKDGWKGP
                     LEGLTMALDRKAVRGILPRGGTILGTSRTNPAKIDSGLERIRDGLAAAGADALIAIGG
                     EDTLGVASQLHEMGVNVVGVPKTIDNDLSATDYTFGFDTAVNIVMEAIDRLHTTAESH
                     HRALIVEVMGRHAGWIALHGGLAGGANVILIPEVPFDIADVADHVSHRFKDHYSSIIV
                     VAEGAAPKEGTMAVQNAEVDAFGHVRLGGIGERVAAEIQERTGKEARTAVLGHIQRGG
                     TPTAFDRWLATRFGLHAIDAVHEGAWGTMVALHGTEIRRVALHEATGTLKVVDPTLYA
                     EAEVFFG"
     gene            1953..3437
                     /gene="glpK"
                     /locus_tag="VLC50_02235"
     CDS             1953..3437
                     /gene="glpK"
                     /locus_tag="VLC50_02235"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HSF26315.1"
                     /translation="MSVLAIDAGTTGVTALVVGHAGDVLARGYEEFAQHFPSPGWVEH
                     APEEIWQATLAAVRSALDQAEAEYGESAAPSCVGVTNQRETAVLWDRDTLVAPRRAIV
                     WQDRRTAEICTELAEAGHDARVAELTGLRLDPYFTATKLAWLARHDEHGWPGVADGRV
                     VVGTVDSYLVARLTGGRTHVTDASNASRTLLFDICRGEWSDELCELFGVPRSALPEVV
                     PSYGVVGRTDPSAFLGLDLPVAGIAGDQQAALFGQACYSPGDSKCTYGTGSFILVNTG
                     AELVRSAGLLTTVAWMTPSREMVYALEGSVFVTGAAVQWLRDGLGLIGSAPETEAIAA
                     TVPDSGGVVFVPALTGLGAPHWDPDARGALLGITRGTTRAHVVRATLEAIAFQVRDVV
                     EVLPPVTPLTALRVDGGASANDLLAQLQADQLGVDVLRPRVRETTGMGAAFLAGLGTG
                     LWSSTEELADTWTLDRRFEPGTRDHAAYARWRAAVERAKGWARP"
     gene            3660..4565
                     /locus_tag="VLC50_02240"
     CDS             3660..4565
                     /locus_tag="VLC50_02240"
                     /inference="COORDINATES: protein motif:HMM:NF037405.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HSF26316.1"
                     /translation="MLPSDIVRALGGHARWHQLHRHVTPRALRLAVRSGQLLHTTRGP
                     YLLPTSPHAARLAADLLGVRSHTSAAQHWQLGLVARPDAEHVTVSSRARRRRKPADVV
                     LHYRDLRPDEVIDDVTSPIRTVLDCARDLPLREALAVGDSALRSGLVDYDELRLLALG
                     LRGPGSARTRQFVTWLDARSANAFESATRAILLEAGVTGFRPQVTIRAHGRFLGRVDL
                     ANVRLRIVIECDGFAWHGQRSALVRDCRRYDELTAHGWAVLRLAWEQVMFEPEWVLAT
                     VQTLVAQRERTLGTDQHPAVNRIAA"
     gene            complement(4623..5411)
                     /locus_tag="VLC50_02245"
     CDS             complement(4623..5411)
                     /locus_tag="VLC50_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007381455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HSF26317.1"
                     /translation="MFYWVLKNFLLGPLLKLFFRPWVEGAENVPEKGPAILASNHLSF
                     SDSVFLPLVLPRQVIFLAKSDYFTGPGLKGRLTALFFKLADQLPIDRSGGRASEAALR
                     TGLRVIGRGELLGIYPEGTRSPDGRLYRGRTGVARMALEAKVPVIPVAMIDTEKLQPP
                     GKVVPHLLRPGVRIGKPLDFSRYEGMEGDRFVLRSITDEIMYELMALSGQEYVDVYAS
                     AMKTRLAEARRAEAAKAKAASVKADGDQSDADAPADPPSAPPAA"
     gene            5607..6380
                     /locus_tag="VLC50_02250"
     CDS             5607..6380
                     /locus_tag="VLC50_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018093508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HSF26318.1"
                     /translation="MPLLPGAEPAQFDGGPVGVVVVHGFTGSPGGVRAWGEYLAEHGL
                     TVAVPRLPGHGTTWQELAHTSWQDWYGEVERTFVELTDRCETVFAMGLSMGATLCLRL
                     AQVHDTGVRGLVLVNPFLSHPSPLLPLLPALRFVLPSVPGVVNDIAKPGQDEVGYSRV
                     PLQALHSVTRLWRHVIPELPSMTRPTLLFRSQTDNVLGARGAAMLLRRIGATDVEERV
                     LPDSHHVATLDYDAPTIFEGSLEFVQRLVPDAEPAGGGS"
BASE COUNT          867 a         2375 c         2339 g          936 t
ORIGIN      
        1 agacgtcgta cggcgtgccg gtgcgcgagt cgatgcgggt cagcgcgccc tcgtagtcga
       61 gcagcagcgc ctcgtgggag gcgtagaggt cgacggtgcg gaacgactcg gggtcggccc
      121 cggcggcctg catgaacgcc aaggcccggc cgatgttgcg ggccatcacc tcgtagcggt
      181 ggtaggcgct ggagccgttg aggaagccgc ggttccactc gtgcacctgg cgcaggtcgg
      241 cgaacccgcc ctgggtgaac gcgcgcagca ggttaagggt ggcggcgctg tggtggtaga
      301 cgtcgagcag ccggcgcggg tcgggctggc gggcggccgc ggtgaacgcc aggtcgttga
      361 cggcgtcgcc gcggtaggac ggcagctcca ccccgtcgcg gacctcggtg tcgcgagagc
      421 gcggcttggc atattgcccg gccagccggc ccagcttgac caccggcagg ctggcgccgt
      481 aggtgagcac cacggccatc tgcaggatcg tcttgatccg gttgcggatg ccgtcggcgg
      541 tgttggcggc gaacgtctcg gcgcagtcgc ccccctgcag caggaacgcc cggccggcgg
      601 ccacctcacc cagcgcggcc ttgaggttgt cgcactcgcc agcgaagacc agcggcggca
      661 gcgcccgcag gctggtcgtg acctcgtcga ccgcggcctg gtcgccccac accggctgct
      721 gggccgccgg ccggtcgcgc cagctcgtcg gcacagtggg cgtcgcggct ggcgactcgt
      781 ggagattcac ccgcccaggg taagcgtcgc cgctagccga agaagacctc agcctcggcg
      841 tagagcgtcg ggtcgacgac cttgagcgtg ccggtggcct cgtgcagcgc gacgcgcctg
      901 atctcggtgc cgtgcagcgc gaccatcgtg ccccaggccc cctcgtggac cgcgtcgatc
      961 gcgtgcagcc caaaccgggt ggccagccaa cggtcgaacg cggtcggggt gccgccgcgc
     1021 tggatgtgcc cgaggaccgc cgtacgcgcc tccttgccgg tgcgttcctg gatctcggcc
     1081 gccacccgct ccccgatgcc gccgaggcgc acgtggccga acgcgtcgac ctcggcgttc
     1141 tgcaccgcca tggtgccctc cttgggcgcg gcgccctcgg cgacaacgat gatcgaggag
     1201 tagtggtcct tgaaccggtg gctgacgtgg tcggccacgt cggcgatgtc gaagggcacc
     1261 tcggggatga ggatcacgtt ggcccccccg gccagcccgc cgtgcagcgc gatccagccg
     1321 gcgtggcggc ccatcacctc gacgatcagc gcccgatggt gcgactcggc ggtggtgtgc
     1381 agccggtcga tggcctccat gacgatgttg accgcggtgt cgaagccgaa cgtgtagtcg
     1441 gtggccgaca ggtcgttgtc gatggtcttg ggcaccccga cgacgttcac gcccatctcg
     1501 tgcagctggg aggcgacccc cagggtgtcc tcgccgccga tcgcgatcag cgcatcggcg
     1561 ccggcggcgg ccagcccgtc acggatccgc tccagcccgg agtcgatctt ggccgggttg
     1621 gtccgcgacg tgccgaggat ggtgccgccg cggggcagga tcccgcgcac cgccttccga
     1681 tccagcgcca tggtcaaacc ctcgagcggc cccttccaac cgtccttgaa cccgacgacc
     1741 tcgtagccat aatcggtgct gcccttgcgg acgatggccc ggatcacggc gttgaggccg
     1801 gggcagtcgc cgccgccagt cagcacaccc acacgcatgc ctcgaccgta atcgcagccg
     1861 gccccgcccc gtgccgctta gggtccgtcc atgcacctcc accccctcgg tgatcaagca
     1921 atccctgccc acgagcccta cggcggcctc cggtgagcgt gctggcgatc gacgcgggca
     1981 cgaccggggt caccgcgctc gtggtcgggc acgcgggcga cgtgctggcc cgcggctacg
     2041 aggagttcgc ccagcacttc ccgtcaccgg gctgggtcga gcacgccccc gaggagatct
     2101 ggcaggccac gctcgcggcc gtccggtcgg cgctggatca ggccgaggcg gagtacggcg
     2161 agtcggcggc cccctcgtgc gtcggggtca ccaaccagcg ggagaccgcg gtgctctggg
     2221 accgcgacac gctggtggcc ccgcgccggg cgatcgtctg gcaggaccgc cgtaccgccg
     2281 agatctgcac cgagctggcc gaggccggcc acgacgcgcg ggtcgccgag ctgaccgggc
     2341 tgcggctgga tccctacttc accgcgacca agctggcctg gctcgcccgc cacgacgagc
     2401 acggctggcc cggcgtcgcg gacggacggg tcgtggtggg cacggtcgac agctacttgg
     2461 tggcccggct caccggcggg cgcacccacg tcaccgacgc gtccaacgcc tcgcgcacgc
     2521 tgctcttcga catctgtcgc ggcgagtggt ccgacgagct gtgcgagctg ttcggcgtcc
     2581 cccggtcggc gctgccggag gtggttccgt cgtacggcgt ggtgggccgc accgacccca
     2641 gcgcgttcct gggcctcgac ctgcccgtcg cgggcatcgc cggtgaccag caggcggcgc
     2701 tgttcgggca ggcctgctac tcccccggcg acagcaagtg cacctatggc accgggtcgt
     2761 tcatcctcgt caacaccggg gccgagctgg tgcgctcggc cgggctgctc accacggtcg
     2821 cctggatgac gccctcgcgg gagatggtct atgcgctgga gggctccgtc ttcgtcaccg
     2881 gcgccgcggt gcagtggctg cgcgacgggc tgggcctgat cggctcggcg ccggagaccg
     2941 aggcgatcgc cgccacggtg cccgacagcg gtggggtggt gttcgtgccc gcgctgaccg
     3001 gcctcggcgc cccgcactgg gaccccgacg cccgcggcgc gctgctcggc atcacccggg
     3061 gcaccacccg cgcccacgtc gtgcgcgcca ccctggaggc gatcgcgttc caggtccgcg
     3121 acgtcgtcga ggtgctgccg ccggtgacgc cgctgacggc gctgcgggtc gacggcgggg
     3181 cctcggccaa cgacctgctc gcccagctgc aggccgacca gctcggcgtg gacgtgctgc
     3241 ggccgcgggt gcgggagacg accgggatgg gcgcggcgtt cctggccggg ctgggcaccg
     3301 ggctgtggtc ctccaccgag gagctggccg acacgtggac gctggaccgc cggtttgagc
     3361 ccggcacgcg cgaccacgcg gcctacgccc gctggcgcgc cgccgtcgag cgagccaagg
     3421 gctgggcccg cccctgacgc cgctcaaacc ccggcgtccc ggacgctagg gacggagaaa
     3481 ccggccgacg cgtggccctc gcaggctcac tcaccgcaac gatggagcgt tgttccgtcc
     3541 ctaccgtccg ccaagaccag ctggaccggt gttgtccaca cccagcgatc gccttgggac
     3601 cgtccacagc cggccttgtc gaagcgctgc gaccgatcct caccagcgac cgtgccggca
     3661 tgcttccaag cgacatcgtt cgggccctgg gcgggcacgc ccgatggcac cagctgcacc
     3721 gccacgtcac tcctcgcgcg ctccggctag cggtcaggtc cggccagctg ctgcacacca
     3781 cccgcggccc atacctcctg ccgaccagcc cgcatgccgc ccggctggct gccgacctac
     3841 tcggcgtgcg ttcgcacacc agcgcggccc agcactggca gctggggctg gtggcacgcc
     3901 ccgacgccga gcacgtcacg gtctctagcc gggcacggcg acgccgcaag cccgccgatg
     3961 tcgtgctgca ctatcgcgac ctgcgccccg acgaggtgat cgacgatgtg acctcaccga
     4021 tccgcacagt gctggactgc gcccgtgacc tcccgttgcg cgaggcgctc gcggtgggcg
     4081 actccgcgct gcgctcgggc ctggtggact acgacgagct ccgcctgctg gcgctcggcc
     4141 ttcgcggtcc cgggtccgcc cggacgcgtc agttcgtcac ctggctcgac gcccggtcgg
     4201 ctaacgcgtt cgagtcggcg acccgggcga tcctgctcga agccggggtc actggattcc
     4261 ggccacaggt gacgatccga gcccacggcc gattcctagg gcgggttgac ctcgccaacg
     4321 tcaggcttcg catcgtgatc gagtgtgacg gcttcgcctg gcacggccag cgctcggcgt
     4381 tggtcaggga ctgtcgccgt tatgacgagc tgaccgccca cggatgggcg gtactgcgac
     4441 tggcctggga acaggtcatg ttcgagcccg agtgggtgct cgcgaccgtc cagacgctcg
     4501 tcgcccagcg cgagcggacg ctaggaacgg accaacaccc cgccgtgaac cgcatcgccg
     4561 cctaggccga gcgcccggca gccgttgatc cgtcccgtgc gtccgcctgc gagcggcgcg
     4621 ggtcaggcgg cgggcggggc agacggtggg tccgcggggg cgtcggcgtc ggactggtcg
     4681 ccgtcggcct tgaccgaggc ggccttggcc ttggcggcct cggcgcgccg cgcctccgcc
     4741 agtcgcgtct tcatcgccga ggcgtagacg tcgacgtact cctgccccga cagcgccatg
     4801 agctcataca tgatctcgtc ggtgatcgag cgcaggacga accggtcgcc ctccataccc
     4861 tcgtagcggg agaagtcgag cggcttgccg atgcgcaccc cggggcgcag caggtggggc
     4921 accactttgc ccggcggctg cagcttctcg gtgtcgatca tcgcgaccgg gatgaccggg
     4981 accttggcct ccagcgccat ccgggccacg ccggtgcgtc cgcgatagag ccggccgtcg
     5041 ggtgagcggg tgccctcggg gtagatgccc agcaactcgc cgcgcccgat gaccctcagc
     5101 ccggtccgca gcgccgcctc actggcccgc ccgccggacc ggtcgatggg caactggtcg
     5161 gccagcttga agaacagcgc cgtcagccgg cccttgagcc cggggccggt gaagtagtcg
     5221 ctcttggcca ggaagatcac ctgccggggc aggacgagcg gcaggaacac cgagtcggag
     5281 aacgagaggt ggttgctggc gaggatcgcc gggcccttct cgggcacgtt ctcggcaccc
     5341 tcgacccagg gccggaagaa cagcttgagc aagggcccga gcaggaagtt cttcaggacc
     5401 cagtagaaca acccgccacc tccatggagt cgaggttcgg acgggacggt gtgtcgcgcg
     5461 tgccacctct gcacgcagac cctagcggcg acggtggccg gcacggcgca cggcggggat
     5521 cgcttcaccc ggccgcggtg cccgtgcgac gatcgggtgg acccggccgg tggtgcgttc
     5581 gcacccgtac gacgaaggag cccgacgtgc cgctcctgcc cggcgccgag cccgcccagt
     5641 tcgacggcgg tccggtgggg gtcgtcgtcg tccacggctt caccggctcg cccggcggcg
     5701 tacgggcctg gggcgaatat ctcgccgagc acgggctcac cgtcgccgta cctcggctgc
     5761 cggggcacgg caccacgtgg caagagctcg cccacaccag ctggcaggac tggtacggcg
     5821 aggtcgagcg cacgttcgtc gagctgaccg atcggtgcga gacggtgttc gcgatggggc
     5881 tgtcgatggg cgccacgctg tgcctgcggc tggcccaggt gcacgacacc ggcgtacgcg
     5941 gcctggtgct ggtcaacccg ttcctctcgc acccgagccc gctgctgccg ctgctgccgg
     6001 cgctgcggtt cgtgctgccg tcggtgcccg gcgtcgtcaa cgacatcgcc aagcccggcc
     6061 aggacgaggt gggctactcc cgggtgccgc tgcaggcgct gcactcggtg acccggctgt
     6121 ggaggcacgt catcccggag ctgccgtcga tgacccggcc gacgctgctg ttccgctcgc
     6181 agaccgacaa cgtgctcggc gcccgtggtg cagccatgct cctgcgacgg atcggcgcca
     6241 ccgacgtcga ggagcgggtg ctgcccgaca gccaccacgt cgccacgctc gactacgacg
     6301 cgccgacgat cttcgagggc agcctggagt tcgtccaacg gctcgtgccc gacgccgagc
     6361 cggccggagg tgggtcatga gcccggcgtc cggccgcggc aacgggctgt cggccgagtc
     6421 ctggacgccg gtcgccgacc tcgacccaca gctggccgac gcgatgctgt cggcgctgcg
     6481 ccaggagggc gtggcggcct acgcggcccc gaccgag
//
LOCUS       DATIBB010000062         3116 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_818909, whole genome shotgun sequence.
ACCESSION   DATIBB010000062 DATIBB010000000
VERSION     DATIBB010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3116
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_818909"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..636
                     /locus_tag="VLC50_02255"
     CDS             <1..636
                     /locus_tag="VLC50_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003982201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HSF26319.1"
                     /translation="TWRPERDRRLLVVLDTGRTSAGRVGDAPRLDAAIDATLLLAALA
                     GHAGDRVDLLAYDRAVRARVARRTGASLLPALVDATATLEPALVEADYRGLVGQVLAA
                     GRHDLVVLLTGLDAAPVEEGLLPVLGPLVSRSRVLVAAVADPRLDELASGRADAAAVY
                     AAAAAERERGERRRVTERLGQLGVTVVDAPPAQIAPALADAYLALKSAARL"
     gene            932..1285
                     /locus_tag="VLC50_02260"
     CDS             932..1285
                     /locus_tag="VLC50_02260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF26320.1"
                     /translation="MDGDSVEVVTARSRDTGMVSAGLKSLLWNTVHTSERGARDTLPT
                     REESQAEANAMAAWLATNPSPSSSLVVAGTAIPALTLTVGGRLGLGADLGIVHVVIVG
                     DPDSVQSYPLIQVTV"
     gene            complement(1306..1902)
                     /locus_tag="VLC50_02265"
     CDS             complement(1306..1902)
                     /locus_tag="VLC50_02265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF26321.1"
                     /translation="MVVRRIVVVALGLVVAVGAGRWAFQDARARSASTPPQDTFADKA
                     AVEAREAARVAELDAIAASRELRAEVLARKEADRAARAAASTAPETVEPWPEGIFEDP
                     EAPFPGMEFLGTNRWVGRVGGHTVAVFAGRSGSDESVGRLQVVVAGRSMNVESGATVA
                     LAGAGALRVVSAEGAMLTIADAHGGTHTFDVLAQRFFS"
     gene            complement(1944..2945)
                     /locus_tag="VLC50_02270"
     CDS             complement(1944..2945)
                     /locus_tag="VLC50_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018559692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HSF26322.1"
                     /translation="MDVDAYVAAHRDEWTRLEALLRRGRRLTPAEVDEMVALYQRAAT
                     HLSAVRSASPDPALVTRLSALVARARAAVTGAPTPAWREVARFVVEVFPVTVYRARRW
                     IVATAVGWLAVAAVVATWVATSPSVQQQIATPEEVRRLVEVDFEGYYSAHPAAAFAAQ
                     VWTNNAWIAAAALAFGVLLGLPVLWLLWQNAANVAVAAGLMAAHDRLGLFFGLITPHG
                     LLELTAVFVAAGLGLRLGWTVIDPGGRPRSVALAAEGRTTLSAALGLVVVLAVSGAVE
                     AFVTPSGLPTWARITIGALVWGLFVAYVAVLGRRGVAAGLTGDLRPVERGEVAPYVA"
BASE COUNT          472 a         1152 c         1090 g          402 t
ORIGIN      
        1 acctggcggc ccgagcgcga ccgccggctg ctggtggtgc tcgacaccgg gcggacctcg
       61 gccgggcggg tcggcgacgc gccacggctg gacgcggcga tcgacgcgac cctgctgctg
      121 gccgcgctgg ccgggcacgc cggtgaccgg gtcgacctgc tcgcctacga ccgggcggtg
      181 cgggcccgag tggcgcggcg gaccggcgcc tcgctgctgc ccgcgctcgt cgacgcgacc
      241 gccaccctgg agccggcgct ggtcgaggcc gactatcgcg ggctggtcgg gcaggtgctc
      301 gccgcagggc ggcatgacct ggtcgtgctg ctcacggggc tcgacgccgc ccccgtcgag
      361 gagggtctgc tgccggtgct tgggccgctc gtgtcccgct cgcgcgtgct ggtggccgcg
      421 gtggccgacc cgcgcctcga cgagctcgct tctgggcgcg ccgacgcggc ggccgtctat
      481 gccgctgcgg ccgccgaacg cgaacgcggc gagcgtcgcc gagtgaccga acgactgggc
      541 cagctcgggg tcaccgtggt cgacgccccg ccggcgcaga tcgccccggc cctggccgac
      601 gcctacctcg cgttgaaatc cgccgcccgc ctctgacccc gccgggggga atgaccacgc
      661 caagtgggcg ttcgcgcact tggcaaggcg tgcacgcctc gccaagtgac agaacgcctt
      721 gcctagctgt aatccagcga cgagtttgcg caggctgcac cattgactgg tgcggggccc
      781 cggcactcca tcgcgtcgac gccttcaccg gctgtcccaa cgcttgggcg ctccgctgct
      841 cggtctgccg cccggcaccg ccacgcgatg gtggatcagc agcgtcctca cagccgatgg
      901 ccaggtgcac gcggtagagc tgacattcct gatggacggc gactccgtcg aggtcgtcac
      961 cgctcgatcc cgcgacacag gaatggtctc tgccggcctg aagtcgttgc tctggaacac
     1021 cgttcacaca tctgagcgag gtgctcgaga caccctgcca acgcgagagg agtctcaagc
     1081 cgaggcaaac gcaatggccg cgtggcttgc gaccaacccc tctcctagct cgtcgctcgt
     1141 cgtggccggc acggcgattc ccgcgttgac actcacggtc ggaggccgcc tgggacttgg
     1201 cgccgacctg gggatcgtcc acgtcgtcat cgtcggggac ccagactcag tccagagcta
     1261 cccgctcatt caggtgaccg tgtgaccccg cgtaagcaac agcggtcagg agaagaaccg
     1321 ctgagcgagt acgtcgaagg tgtgcgtgcc accgtgggcg tcggcgatcg tcaacatcgc
     1381 gccctcggcc gagaccaccc gcagcgctcc ggcgccggcc agggcgacgg tggcgccaga
     1441 ctcgacattc atcgaccgcc cggcgactac gacctgcagc ctgcccacgg attcgtcact
     1501 gccagaccga ccggcgaaca ccgcgacggt gtgcccgccg actcggccga cccatcggtt
     1561 ggtacccagg aactccatgc ctgggaaggg agcctcgggg tcctcgaaga tgccttcagg
     1621 ccaaggttcg acggtctccg gggcggtcga cgccgcagcc cgggcagcgc ggtctgcctc
     1681 cttgcgcgca aggacctcgg cacgcagctc ccgcgaggca gcgatggcat ctagctcggc
     1741 gacgcgggcg gcctcgcgag cctccacggc cgccttgtcg gcgaacgtgt cctgcggtgg
     1801 ggtcgaagcg ctgcgggccc gcgcatcctg gaacgcccac cttcctgcgc ccacggcgac
     1861 aaccagccca agcgcaacaa ccacgatccg acgcaccacc atgacgtccc ccgtcgacgg
     1921 cgacgacgat caatgtatga gtgtcaggcg acgtacggcg cgacttcgcc tcgctcgacc
     1981 ggacgcaggt caccggtcag acccgccgcg acaccacgcc gaccgagcac cgcgacgtac
     2041 gccacgaata gcccccacac gagcgcgccg atggtgatgc gcgcccaggt cggcaggccg
     2101 ctcggtgtga cgaacgcctc caccgcgccc gagacagcga gcaccacgac cagcccgagc
     2161 gcggccgaca gcgtcgtacg cccctcagcc gccagcgcca ccgaccgcgg gcgaccgccc
     2221 gggtcgatca ccgtccagcc cagccgcagc cccagcccgg cggccacgaa gactgcagtc
     2281 agctcgagca gcccgtgcgg ggtgatcagc ccgaagaaca acccgagccg gtcgtgggcg
     2341 gccatcaacc cggcggccac cgcgacgttg gcggcgttct gccacagcag ccacagcacc
     2401 ggcagcccca ggagtacgcc gaaggcgagc gcggcggcgg cgatccaggc gttgttggtc
     2461 cacacctgcg cggcgaacgc ggcggccgga tgggcggagt agtagccctc gaagtcgacc
     2521 tcgacgagtc ggcgtacctc ttcgggggtc gcgatctgct gctgcacgga cggcgaggtg
     2581 gccacccacg tcgccaccac cgcagcgacc gccagccacc cgaccgcagt cgcgacgatc
     2641 cagcggcggg cccgatagac ggtgaccgga aagacctcga cgacgaagcg ggccacctcg
     2701 cgccaggccg gggtcggcgc gccggtgacc gcggcccgag cccgggcgac cagtgcggac
     2761 agccgggtga ccaaggcggg gtcgggtgag gcggagcgta cggcggacag gtgggtggcg
     2821 gcgcgctggt agagcgcgac catctcgtcg acctcggcgg gcgtcaggcg ccggccgcgg
     2881 cgcagcagcg cctcgagccg ggtccactcg tcgcggtgcg ccgcgacgta ggcgtcgacg
     2941 tccacggccg tcctcccgtc gggtcgcaga ctagcctgca caagatgagc ggggtggtga
     3001 ccggcgaggc ggtcgtcgtc gagctcgacg cggcccgggt gccgacccgg gcgatcgcgt
     3061 tcggcatcga cgcgacaatt caggtcctgc tgctggtgct cgggacgttc ctggtc
//
LOCUS       DATIBB010000063         5536 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_1869939, whole genome shotgun sequence.
ACCESSION   DATIBB010000063 DATIBB010000000
VERSION     DATIBB010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 5536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5536
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1869939"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1825)
                     /locus_tag="VLC50_02275"
     CDS             complement(<1..1825)
                     /locus_tag="VLC50_02275"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HSF26323.1"
                     /translation="MARPGDRLARWWAGWRVALRMTRRDVRRHKGRAALVVLLVGLPV
                     LLISAGATLLATDSVEPAEEIPARMGQTQASIAYRPYLFDEAEAADGQAAHHEQALAD
                     LPRLVDGTLIPTTMGDSRVVMGERKVAVTILGVDGRNPVTAGMVSLTSGRWPAADDEV
                     LVTPLALAKGIPASGTLTLTTPEGPARTVTVVGQGSAGGHFQRALITLPTISGDNEVS
                     YLVDRADPVTWRDVKALNAAGYDVTSRAVMLDPSEAKADPEYDGRFDNGNSAEVVGLV
                     ALIAVGMVLETALLAGPAFAVGAARSRRALALAAANGADKRQLRRHVLGQALLLGGLA
                     AVVGATLGPLAARVGIGLWTRTHPEHNLAAVFDVPLGQVLAIVVCAVLASLAAAFAPA
                     VGAARLDLVAILRGHRAHRPAHRGMPVLGLVIATVGGAVVVNAIVNRGREMSVAGGAV
                     LLVGGTLLLIPMLLSLVGRWGARLPLPLRMAARDIGRQRGRAVPTVGAIMAAVATLTT
                     LAIGGISDEAQGARDYQPQAPIGHGLLQGNPVDLADARAVVADIDPELSIVDIRALVP
                     DRSVIDPTTGREGIIAVLPPGCSDRDALDWTSACSLDEQVPP"
     gene            complement(1828..2562)
                     /locus_tag="VLC50_02280"
     CDS             complement(1828..2562)
                     /locus_tag="VLC50_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019432088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSF26324.1"
                     /translation="MSASAVVLQLEGVSRVHGQGAAQVHALTDVSLTVAAGELVAVMG
                     PSGSGKSTLLNLAGGLDRPTSGQVLVEGEDVARLGAAGLSRLRRRRVGFVFQDFNLIP
                     SLTAAENVALPLELDGATARGARHAAIAALESVDIGELSDRFPDELSGGQQQRVAIAR
                     ALVGDRRLVLADEPTGALDSHTGEAVLAVLRERCDAGAAGLLVTHESRHAAWADRVVF
                     LEDGVIVDTTGTPPTAEGLFDETPAV"
     gene            complement(2559..3152)
                     /locus_tag="VLC50_02285"
     CDS             complement(2559..3152)
                     /locus_tag="VLC50_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003801228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HSF26325.1"
                     /translation="MSIKQGLLALLAEAPMYGAQLRSEFEARTGGTWPLNVGQVYTTL
                     GRLERDGLVEEGPADAEGRITYRLTETGRAEAERWWVSPVDRDPAPRNELAIKLALAV
                     TVPGVDVPRVVRTQRTATVRYLQDLTRLKRRTAKDDPARDLAWRLVLDNLVYAAEAEV
                     RWLDHVEASLARASASTRGSARKASGGTPVGKARSAQ"
     gene            3276..3647
                     /locus_tag="VLC50_02290"
     CDS             3276..3647
                     /locus_tag="VLC50_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013284343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HSF26326.1"
                     /translation="MHHFSIATVAEQSPDFRRVLWTGAHSQLVIMTIPPGGEIGQEVH
                     AENDQILTFVSGTGEAIVAGETKPLVPGDLVAVPAGTEHNFRNTGANPLVLYTVYGPP
                     EHADGVVHATKAEADAAEHAG"
     gene            complement(3674..4360)
                     /locus_tag="VLC50_02295"
     CDS             complement(3674..4360)
                     /locus_tag="VLC50_02295"
                     /inference="COORDINATES: protein motif:HMM:NF024791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR C-terminal domain-containing protein"
                     /protein_id="HSF26327.1"
                     /translation="MSMYTPPGRGARSSRRRRSGVLRHAIVLVLALVLVVGAGVGAWW
                     FTLRSQPAAPLPACPTPTVTVAAPISPAPVAEVKVNVYNATSRQGLAGRVSTALKERG
                     IAVLKVANDPLKKTVPGTAEIRFGPAGESAAETVRLLIDGATLVVDKRTDATVDVVLG
                     QGYTTLRTAEEAAAAATAAPTPPAGDATGGATAPTTAPTATATCVPVPPVTVTAPPPS
                     APAATPATTP"
     gene            4453..>5536
                     /locus_tag="VLC50_02300"
     CDS             4453..>5536
                     /locus_tag="VLC50_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010352267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like domain-containing protein"
                     /protein_id="HSF26328.1"
                     /translation="MAKPRGRHGEEPHDAVAEVTAGYAFDGPALHLGALVVDDAAMPE
                     AAVRIPLAMLNRHGLVAGATGTGKTKTLQLMAEQLGAAGVPVFLADLKGDLSGLASPG
                     VADERLSQRATEVGQAWTPTAAPVEFFSLGGLGSGIPLRATMTSFGPVLLAKVLGLNA
                     VQESSLGLIFHFADQAGLPLLDLKDLRAVVGFLLSEEGKSALKGLGGLASATAGVILR
                     QLVALEDQGADVFFGEPEFDTLDLLRTTADGRGVVSVLELPAVAERPALFSTFLMWLL
                     ADLYADLPEVGDLDAPKLVFFFDEAHLLFDGASKAFLDAVEQTMRLIRSKGVGVFFVT
                     HTPRDVPRDVLAQLGNRVQHALRAYTP"
BASE COUNT          725 a         2016 c         2064 g          731 t
ORIGIN      
        1 cgggcgggac ctgctcgtcg aggctgcagg ccgacgtcca gtcgagcgca tctcggtccg
       61 agcagcccgg tggtaggacg gcgatgatcc cctcgcggcc cgtggtgggg tcgatgaccg
      121 agcggtccgg gaccaatgcg cggatgtcga cgatcgaaag ttcgggatcg atgtcggcga
      181 ccacggcacg ggcgtcggcg aggtcgaccg ggttgccctg cagcaggccg tggccgatcg
      241 gcgcctgcgg ctggtagtcg cgcgcgccct gtgcctcgtc gctgatgccg ccgatggcca
      301 gggtggtgag cgtggcgacc gccgccatga tcgctccgac ggtcgggacg gcccggccgc
      361 gctggcgacc gatgtcgcgg gcggccatcc gcagcggcaa cggcagccgc gccccccagc
      421 ggcccaccag cgacagcagc atcgggatga gcagcagcgt gccgccgacc agcagtacgg
      481 caccaccggc gaccgacatc tcccggccgc ggttgacgat cgcattgacg acgaccgcac
      541 caccgacggt ggcgatcacg agccccagca ccggcatgcc ccggtgcgcg ggccggtggg
      601 cacggtgccc gcgcaggatc gcgaccaggt cgagccgggc ggcgccgacc gcgggggcga
      661 acgccgcggc caacgacgcg agcacggcgc acacgacgat ggccagcact tgcccgagcg
      721 ggacgtcgaa gaccgccgcc aaattgtgct cgggatgcgt gcgggtccac agcccgatgc
      781 cgacccgggc ggccagcggg ccgagcgtcg caccgaccac cgcggccagc ccaccgagca
      841 gcagcgcttg accgagcacg tgtcggcgca gctgtcgctt gtcggcgccg ttggccgcgg
      901 ccagggccag tgcgcgccgt gatcgcgcgg ccccgacagc aaacgcaggc ccggccagca
      961 gcgcggtctc cagcaccatc ccgaccgcga tcaaggccac caggccgacc acctcggcgc
     1021 tgttgccgtt gtcgaagcgg ccgtcgtact ccggatcggc cttggcctcg ctcgggtcga
     1081 gcatcacggc gcggctcgtc acgtcgtagc cggccgcgtt gagtgccttg acgtcgcgcc
     1141 acgtcacggg gtcggcgcgg tcgacgaggt aggagacctc gttgtcgccg ctgatcgtcg
     1201 ggagggtgat gagcgcgcgc tggaagtggc cgccggcact gccctgcccc acaacggtca
     1261 ccgtgcgcgc cggcccctcg ggtgtggtca gggtcagcgt gccagaggcg ggaatgcctt
     1321 tggccagcgc cagcggggtc acgagcacct cgtcgtcggc ggccggccag cggccagagg
     1381 tgagcgagac catgccggcg gtgacgggat tgcgcccgtc gacgccgagg atcgtgaccg
     1441 cgaccttgcg ctcgcccatc acgacccgcg agtcccccat ggtcgtgggg atcagcgtgc
     1501 cgtcgaccag cctcggcagg tcggcgaggg cctgctcgtg gtgggcggcc tggccgtccg
     1561 ccgcttcggc ctcgtcaaac aagtacggcc ggtaggcgat gctggcctgg gtctggccca
     1621 tccgggccgg gatctcctcg gccggctcga cgctgtcggt ggccagcagc gtcgcgccgg
     1681 cgctgatcag cagcaccggc agacccacca gcagtacgac gagcgcggcg cggcccttgt
     1741 gccgtcgtac gtcgcgccgg gtcatccgca gcgcgacccg ccagccggcc caccaccgcg
     1801 ccagccgatc gccaggacgg gccacggtca gaccgccggg gtctcgtcga acaggccctc
     1861 ggcggttggc ggggtgccgg tggtgtcgac gatcacgccg tcctcgagga acaccacccg
     1921 gtcggcccag gcggcgtggc gcgactcgtg ggtgaccagc agcccggccg cgccggcgtc
     1981 gcagcgctcg cgcagcacgg ccagcacggc ttcgccggtg tgcgagtcca gcgccccggt
     2041 cggctcgtcg gcgagcacca gtcgacggtc gccgaccagc gcccgggcga tcgcgacccg
     2101 ctgctgctgc ccgccggaga gctcgtcggg gaaccggtcg gacagctcgc cgatgtcgac
     2161 cgactccagc gcggcgatcg ccgcatgccg ggcgccccgg gcggtggcgc cgtcgagctc
     2221 gagcgggagc gcgacgtttt ccgccgcggt cagcgacggg atgaggttga agtcctggaa
     2281 gacgaacccg acgcgccgcc gtcgcagccg ggacagcccc gccgcaccga gccgggcgac
     2341 gtcctcgcct tcgacgagca cttgcccgct ggtcggccgg tcgaggccac cggccaggtt
     2401 gagcaaggtc gacttgcccg acccgctcgg gcccatcacc gcgaccagct cgccggcggc
     2461 cacggtcagc gagacgtcgg tcagggcatg cacctgggcg gcgccctgcc cgtgcacccg
     2521 ggagaccccc tcgagctgga gcaccacggc cgacgcgctc actgggcgct cctggccttg
     2581 ccgaccggcg tgccgccgga cgccttgcgg gcggacccgc gggtgctggc ggaggcgcgg
     2641 gccaggctcg cctcgacgtg gtcgagccag cgcacctcgg cctcggcggc gtagacgagg
     2701 ttgtccagga ccagccgcca ggccaggtcg cgcgccgggt cgtccttcgc cgtacgccgc
     2761 ttgagccggg tcaggtcctg caggtagcgc accgtggcgg tgcgctgggt ccgcaccacc
     2821 cggggtacgt cgacgccggg caccgtcacc gcgagcgcca gcttgatcgc cagctcgttg
     2881 cggggggcgg ggtcgcggtc gaccggtgag acccaccagc gctcggcctc ggcccgcccg
     2941 gtctcggtca gccggtaggt gatccggccc tcggcgtcgg ccgggccctc ctcgaccagc
     3001 ccgtcgcgct cgagccggcc gagcgtcgtg tagacctgcc cgacgttgag cggccaggtg
     3061 ccgccggtgc gcgcctcgaa ctccgagcgc agctgcgcgc cgtacatcgg cgcctcggcc
     3121 agcagcgcga gcagaccttg cttgatcgac atgcaatgac cgcctctgtg gcatacccgg
     3181 tattccgagt gctaggtata ccgggtattg cggcgcgcca caaggacgct ggctggctag
     3241 cctgagcgga cgcgggaagc cgaaggggga cgatcatgca tcacttctcc atcgccacgg
     3301 tcgccgagca gagccccgac ttccgccggg tcctgtggac gggcgctcac tcccagctag
     3361 tgatcatgac gatcccgccg ggcggggaga tcgggcagga ggtccacgcg gagaacgacc
     3421 agatcctcac gttcgtcagc ggcaccggcg aggcgatcgt ggccggcgag accaagccgc
     3481 tcgtgccggg cgacctggtc gcggtgccgg cgggcacgga gcacaacttc cgcaacaccg
     3541 gggccaaccc gctggtcctc tacacggtct acgggccgcc cgagcacgcc gacggggtcg
     3601 tgcacgcgac caaggccgag gccgacgcgg ccgagcacgc gggctgaccc ggcgcggcga
     3661 gccggttcgc gagctagggc gtcgtcgccg gggttgctgc gggggcgctg ggcggcgggg
     3721 cggtgacggt caccggcggg accggcacac aggtcgcggt tgccgtagga gctgtggtcg
     3781 gtgccgtcgc gcctccggtc gcgtcgccgg caggcggggt cggcgccgcc gtcgctgcgg
     3841 ccgccgcctc ctccgccgta cgcagtgtcg tgtagccctg gccaaggacc acgtcgacgg
     3901 tggcgtcggt gcgcttgtcg acgaccaggg tggcgccgtc gatgagcagc cgcaccgtct
     3961 cggccgccga ctcgccggcc ggcccgaacc ggatctccgc cgtaccgggc acggtcttct
     4021 tcagcgggtc gttggcgacc ttgagcacgg cgatcccgcg ctccttgagc gcggtcgaga
     4081 cccgaccggc caggccctgg cgggaggtcg cgttgtagac gttgaccttg acctcggcca
     4141 ccggcgccgg gctgatcggg gcggcgacgg tcacggtcgg ggtggggcag gcgggcagcg
     4201 gagcggccgg ctgggagcgc agcgtgaacc accacgcccc gaccccggcg ccgacgacga
     4261 ggacgagggc cagcacgagc acgatggcgt gacgcaggac gccgctgcgc cgtcgccgcg
     4321 acgaccgggc gccgcggccc ggtggggtgt acatgctcat gggcgggctg tccgtttcgt
     4381 ccgcatgggc gccaagggta gtgccgacgc gtcgcccggt cagggacgtt gcgtcgggct
     4441 actctcgcgg ccatggcgaa gccgcggggt cggcacggcg aagaacccca cgacgcggtc
     4501 gccgaggtca cggcgggcta cgccttcgac ggccccgcgc tgcacctggg cgcgctggtc
     4561 gtcgacgacg cggcgatgcc ggaggccgcc gtacgcatcc cgctggccat gctcaaccgg
     4621 cacggcctgg tcgccggggc caccggcacc ggcaagacca agaccctgca gctgatggcc
     4681 gagcagctcg gcgcggccgg ggtgccggtg ttcctggccg acctcaaggg cgacctgtcc
     4741 gggctggcct cgcccggggt ggccgacgag cggctgagcc agcgggccac cgaggtcggc
     4801 caggcgtgga cgccgaccgc ggcgccggtg gagttcttct cgctcggcgg gctcgggtcg
     4861 ggcatcccgc tgcgggccac catgacctcg ttcggcccgg tgctgctggc caaggtgctt
     4921 gggctcaacg ccgtgcagga gtcctcgctc gggctgatct tccacttcgc cgaccaggcc
     4981 gggctgccgc tgctcgacct caaggacctg cgcgcggtcg tcgggttcct gctctccgag
     5041 gagggcaagt ccgcgctcaa ggggctcggt ggactggcct cggccaccgc cggggtgatc
     5101 ctgcgccagc tggtcgcgct ggaggaccag ggtgccgacg tgttcttcgg cgagccggag
     5161 ttcgacaccc tcgacctgct gcgtacgacg gccgacgggc gcggcgtcgt ctcggtgctc
     5221 gagctgcccg cggtggccga gcggccggcg ctgttctcga cgttcctcat gtggctgctc
     5281 gccgacctct acgccgacct gcccgaggtc ggagacctcg acgcgcccaa gctggtgttc
     5341 ttcttcgacg aggcgcacct gctcttcgac ggggcgtcca aggcgttcct cgacgcggtc
     5401 gagcagacga tgcggctgat ccggtccaag ggtgtggggg tcttcttcgt cacccacacc
     5461 ccgcgcgacg tgccccgcga cgtgctcgcc cagctgggca accgggtcca gcacgcgctg
     5521 cgcgcctaca cccccg
//
LOCUS       DATIBB010000064         2269 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_30066, whole genome shotgun sequence.
ACCESSION   DATIBB010000064 DATIBB010000000
VERSION     DATIBB010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2269
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30066"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..166)
                     /locus_tag="VLC50_02305"
     CDS             complement(<1..166)
                     /locus_tag="VLC50_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017542192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HSF26329.1"
                     /translation="MAPYGDRVVGVGPWEGPWPTHPRYDPELLAGGDRRNVVDGYRYW
                     RREAIVADLDT"
     gene            262..1008
                     /locus_tag="VLC50_02310"
     CDS             262..1008
                     /locus_tag="VLC50_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018834160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HSF26330.1"
                     /translation="MPFTGDLAAALAPLTVAPSGLGPDWLDPQHLLDGVGLWAVVIVG
                     VILFAECGLLVGFFLPGDSLLFTAGLLISSGALKAPLWLLLVVGTVAAVAGNLVGYWI
                     GREAGPAIFNKPNSRLFRQEYVDKTYSFFDHYGSRAIVLARFVPIVRTFITVMAGVGK
                     MDFRRYATFTAIGGVLWAAGVTVLGYFLGQVAFIRDNLEMIFLAIVAISVIPIAIELM
                     RHRSRRPAARAAAQPDAQPAPVEDPAPPAG"
     gene            complement(1013..2173)
                     /locus_tag="VLC50_02315"
     CDS             complement(1013..2173)
                     /locus_tag="VLC50_02315"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSF26331.1"
                     /translation="MTALTITSATLRRMLRDRVSLFFIVLLPIVVILIVGATVGGFDT
                     FRAGVVTRDTGPLATSLVEDLKASSTLRVRPVDDEAGARTAVRRGELDLLVLIPAGFD
                     ATLSSGAAATVTVLAGGAPGTAQAARAAVDSVVAHHAARVQAARFATDRVGGDLATNL
                     ALAARAQADTPAVTVRTEAVERQSGILPTGFSYSAPTMLVLFVFINALAAGAAIIQTR
                     RLGIYDRALAAPVTARTIVLGETMAYFALAMLQSLLIVGVGAWVFGVSWGDPLATVLL
                     VLAWALVGTGAGMLSGTLFRTPEQAGAIGPAAGIALGMLGGCMWPLAIVTPLMRTLGH
                     AAPHAWAVDGWTTVLSADGGVGDIVVPLAVLTGFAVVLLALAMVRMRRRLVA"
BASE COUNT          313 a          856 c          803 g          297 t
ORIGIN      
        1 gggtgtcgag gtcggccacg atcgcctcgc gccgccagta gcggtagccg tcgacgacgt
       61 tgcgccggtc accgcccgcg agcagctcgg ggtcgtagcg cgggtgggtc ggccacggcc
      121 cctcccacgg acctacgccc acgacgcggt cgccgtacgg cgcgatctcc ctcgcaccac
      181 cctcggtgct cgcgtcctca cccacgggcg accacagtag gtcagcggtc gacctcccag
      241 gcgccggtta gcctggggcc catgccgttc accggggacc tcgcagccgc gctcgccccc
      301 ctgaccgttg ccccgtccgg gcttgggccc gactggctcg acccgcagca cctgctcgac
      361 ggggtcggcc tgtgggcggt cgtcatcgtc ggggtgatcc tgttcgccga gtgcgggctg
      421 ctcgtcgggt tcttcctgcc gggcgactcg ctgctgttca cggccggact gctcatctcc
      481 agcggggcgc tgaaggcccc gctgtggctg ctgctcgtcg tcggcacggt cgcggcggtc
      541 gcgggcaacc tggtcggcta ctggatcggg cgcgaggccg gcccggcgat cttcaacaag
      601 cccaactcgc ggctgttccg ccaggagtac gtcgacaaga cctactcatt cttcgaccac
      661 tacggcagcc gcgcgatcgt gctggcccgg ttcgtgccga tcgtgcgcac gttcatcacc
      721 gtgatggccg gcgtcggcaa gatggacttc cgccgctacg cgaccttcac cgcgatcggc
      781 ggcgtgctgt gggccgccgg cgtgaccgtg ctcggctact tcctcggcca ggtggcgttc
      841 atccgcgaca acctcgagat gatcttcctg gccatcgtcg cgatctcggt catcccgatc
      901 gcgatcgagc tgatgcgtca ccggtcccgc cggcccgccg cacgcgctgc ggcgcagccg
      961 gatgcgcagc ccgccccggt cgaggacccg gcacccccgg ccggttgatc cctcaggcga
     1021 ccagccgtcg gcgcattcgc accatcgcca gggcgagcag caccaccgcg aacccggtca
     1081 ggacggcaag cggtacgacg atgtcgccga ccccgccatc ggcggagagg acggtcgtcc
     1141 agccgtcgac cgcccaggcg tgcggggccg cgtgccccag cgtgcgcatc agcggcgtga
     1201 cgatggccag cggccacatg cacccaccga gcatgcccag ggcgatcccg gcggcagggc
     1261 cgatcgcccc ggcctgctcg ggggtgcgga acagggtgcc cgacagcatc ccggctccgg
     1321 tgcccaccag cgcccaggcg agcacgagca ggaccgtggc gagcgggtcg ccccagctga
     1381 cgccgaacac ccacgcgccg accccgacga tgagcaacga ctgcagcatc gcgagcgcga
     1441 agtaggccat cgtctcgccg agcacgatgg tgcgggcggt caccggcgcg gccagcgccc
     1501 ggtcgtagat gccgagccgg cgggtctgga tgatcgccgc gccggcggcc agcgcgttga
     1561 tgaacacgaa cagcaccagc atcgtcggag cgctgtagga gaacccggtc ggcaggatgc
     1621 cgctctggcg ctcgactgcc tcggtgcgca ccgtgaccgc cggggtgtcg gcctgggccc
     1681 gggccgccag cgccaggttg gtcgcgagat ccccgccgac ccggtcggtg gcgaagcgag
     1741 ccgcctgcac gcgggcggcg tggtgggcga cgaccgagtc gaccgcggcc cgggcggcct
     1801 gcgcggtgcc cggcgcccca ccagcgagca ccgtgaccgt cgcggctgcc cccgagctca
     1861 gcgtcgcgtc gaagcccgcc gggatgagca ccagcaggtc gagctcccct cggcgtacgg
     1921 cggtgcgcgc gccggcctcg tcgtcgaccg ggcgcacccg cagcgtcgag gaggccttca
     1981 ggtcctcgac cagcgaggtg gccaacggcc cggtgtcgcg ggtgaccacg ccggcccgga
     2041 atgtgtcgaa gccgccgacc gtggcgccca cgatgaggat gacgacgatg ggcagcagca
     2101 cgatgaagaa cagtgagacc cggtcgcgca gcatccgccg cagcgtcgcg ctggtgatcg
     2161 tcagcgcggt catcggcgca ccgcccgccg gcccagcgtg gccgcggcca gccccgccag
     2221 cacggcgaag gccacgatgg ccgcgaccgg caccagccag gtgctcacg
//
LOCUS       DATIBB010000065         4908 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_1870739, whole genome shotgun sequence.
ACCESSION   DATIBB010000065 DATIBB010000000
VERSION     DATIBB010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 4908)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4908)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4908
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1870739"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..954)
                     /locus_tag="VLC50_02320"
     CDS             complement(<1..954)
                     /locus_tag="VLC50_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014848023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HSF26332.1"
                     /translation="MTVTVRPISAPAHLEVIAARGAASFLQTPAWGAVKRDWRPQLLG
                     FFRDRQTTPFGAGLVLYRQVPRLRRYLAYLPEGPVLDWDAMDPTAIGESLTALVAHVK
                     AQHAFGLRIGPPVVTRRWSAATVKDGISAGAATHLPDLPADETTPVGHRVAAALARAG
                     FRPPQDNGGFSAGQPRYVFQVPLAGRSDDELLAGFNQLWRRNIRKAAKAGVVVRQGCA
                     DDLGAFHRLYAETAARDGFTPRPLGYFQGMWEAMHAEDPDRLRLYLAHHDGDLVAATT
                     MVQVGTHAWYSYGASSTAKREVRGSNAVQWQMMRDARDAGAT"
     gene            complement(1131..1685)
                     /locus_tag="VLC50_02325"
     CDS             complement(1131..1685)
                     /locus_tag="VLC50_02325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF26333.1"
                     /translation="MRKPRTALMTVVAVAATASLSLGGVALAATGLPGSADDHAAEQV
                     AELTIPGPAEASDGHADQRGRSVVPTSDETQAPVADDDSFEAADPATSDTESGDEAQT
                     PEPRPTDTHGYDVSQLATTTAATGRDKGAEISAEARTNRPAGDETEVADEDEETVDAT
                     SSDTPQRSETGEAARSEHAKADAR"
     gene            complement(1790..2596)
                     /locus_tag="VLC50_02330"
     CDS             complement(1790..2596)
                     /locus_tag="VLC50_02330"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006592769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HSF26334.1"
                     /translation="MPSPARTRVGAHVGRIDEIDLVAEARSRGADCVQIFVSDPQDWK
                     ATPLDGVDAEAVRRAAAEADIAVYVHAPYVINVATTNNRIRIPSRKLLQQVVTAAGAI
                     GAAGVIVHGGHVTSGADAAAGFANWAKALDRLEPGAPVLIENTAGGNGAMARTVEQLE
                     RLWAAVGHTDVGLCLDTCHAFSAGIELAEVVERFRAVTGRVDLVHANDSRDPFGSARD
                     RHADLGEGEIGAEPVAAVVAAAGCDAVLETPGVKAGHAQDIAWLREQLTR"
     gene            complement(2623..2919)
                     /locus_tag="VLC50_02335"
     CDS             complement(2623..2919)
                     /locus_tag="VLC50_02335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF26335.1"
                     /translation="MAHTLHPRPRLTLNTDGHRHPRENTLTAITLVAAIISLTCAFVP
                     SWHLLGGWAGLIGMLTGGVAQYLSATTAQRMIVVPALIGSAVGLAFGLHNGGLW"
     gene            3087..3374
                     /gene="rpsF"
                     /locus_tag="VLC50_02340"
     CDS             3087..3374
                     /gene="rpsF"
                     /locus_tag="VLC50_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020557172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HSF26336.1"
                     /translation="MRRYELMVILDPDLEERTVAPSLDQFLTVVRNGGGSVEKVDVWG
                     RRRLSYEIAKKAEGIYAVVDLTAEPDVVKELDRQLNLNEAVLRTKVMRPDK"
     gene            3465..3998
                     /locus_tag="VLC50_02345"
     CDS             3465..3998
                     /locus_tag="VLC50_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009336064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HSF26337.1"
                     /translation="MAGETTITVIGNLVDDPELRFTPSGAAVAKFRIASTPRTFDRQT
                     NEWKDGESLFLTVNIWRQAAENVAESLQRGMRVIVSGRLRQRSYETREGEKRTVYEIE
                     ADEVGPSLRNATAKVTKTSRGGGGFGGSAGASGGSSGGASGGFGSSGGDDPWSTPAPS
                     SGGGGGWGGGSTDQPPF"
     gene            4067..4303
                     /gene="rpsR"
                     /locus_tag="VLC50_02350"
     CDS             4067..4303
                     /gene="rpsR"
                     /locus_tag="VLC50_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003949403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HSF26338.1"
                     /translation="MAKPPARKPKKKVCVFCKEKISHVDYKDTALLRKYISDRGKIRA
                     RRVTGNCTQHQRDIATAVKNSREMALLPYTSTAR"
     gene            4319..4765
                     /gene="rplI"
                     /locus_tag="VLC50_02355"
     CDS             4319..4765
                     /gene="rplI"
                     /locus_tag="VLC50_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013736574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HSF26339.1"
                     /translation="MKLILTQEVSGLGAPGDVVEVKDGYGRNYLVPRGLAIRWTRGTE
                     KQIASIKKAREVREVRDLGHAQEIKGQLEALKVKLPVRAGSGGRLFGSVTVADLVAAV
                     KSAGGPDIDKRKIEVGNPIKTVGAHTVAVRIHPEVAATLHVEVVAG"
BASE COUNT          687 a         1740 c         1793 g          688 t
ORIGIN      
        1 ggtggcgccg gcgtcgcggg cgtcgcgcat catctgccac tgcaccgcgt tggacccgcg
       61 cacctcacgc ttggcggtcg aggaggcgcc gtaggagtac caggcgtggg tgccgacctg
      121 gaccatggtc gtggcggcga caaggtcgcc gtcgtggtgg gccaggtaga gccgcagccg
      181 gtcggggtcc tcggcgtgca tcgcctccca catgccctgg aagtagccca gcggtcgcgg
      241 ggtgaacccg tcgcgtgcgg cggtctcggc gtagagccgg tggaacgcgc cgaggtcgtc
      301 ggcgcagccc tggcgtacga cgaccccggc cttggcggcc ttgcggatgt tgcgccgcca
      361 cagctggttg aacccggcca gcagctcgtc gtcggagcgc ccggccagcg gcacctggaa
      421 cacgtagcgc ggctgcccgg cgctgaaccc gccgttgtcc tggggcgggc ggaacccggc
      481 ccgggccagc gcggcggcga cccgatgccc gaccggggtg gtctcgtcgg ccggcaggtc
      541 aggcagatga gttgcggcgc cagcgctgat gccgtccttg acggtggccg cgctccaccg
      601 tcgggtcacc accggcggac cgatccgcag cccgaacgcg tgctgggcct tgacgtgggc
      661 caccagggcg gtcagtgact cgccgatcgc ggtcgggtcc atcgcgtccc agtcgaggac
      721 cgggccctcg gggaggtagg cgaggtagcg gcgcagccgc ggcacctggc ggtagagcac
      781 cagcccggcg ccgaacgggg tggtctggcg gtcgcggaag aagccgagca gctgggggcg
      841 ccagtcgcgc ttgaccgcgc cccaggccgg ggtctgcaag aagctggccg cgccgcgagc
      901 ggcgatgacc tcgaggtgtg ccggcgcgct gatcgggcgc acggtgacgg tcatcgggct
      961 ccaggcgcgg caacggtgac gaaggagagc aatcgctcag cgccttgcag gctagccgaa
     1021 tccgcacgga tgtggcgccg ggcgcggtcc cggtcgttct acccacgcgg ccggtggcga
     1081 atcacctcgc caccggccgc gtcgatgcgc ctactgggag aaaccgaggg tcagcgcgcg
     1141 tcggccttgg cgtgctcgct gcgtgccgct tcgccggtct ccgagcgctg cggcgtgtcg
     1201 ctgctggtcg cgtccaccgt ctcctcgtcc tcgtcggcga cctcggtctc gtcgccggcc
     1261 gggcggttgg tccgagcctc ggccgagatc tcggcgccct tgtcgcgtcc ggtcgcggcg
     1321 gtggtggtgg ccagctgcga cacgtcgtag ccgtgggtgt cggtcggtcg cggctctggt
     1381 gtctgggcct cgtcaccgct ctcggtgtcg ctggtcgccg ggtcggcggc ctcgaagctg
     1441 tcgtcgtcgg cgacgggcgc ctgggtctcg tcgctggtcg gcacgaccga gcggccacgc
     1501 tggtcggcgt gcccgtcgga ggcctcggcc ggcccgggga tggtgagctc ggccacctgc
     1561 tcggcggcgt ggtcatcggc gctgccgggc aggccggtcg cggcgagcgc gacacctccc
     1621 aggctcagcg acgccgtcgc ggccacggcg acgacggtca tgagggcggt tcggggcttg
     1681 cgcatcggct aggtcctcct cgtgcgcggc gttcagtaca cacacatatc ggacaagctg
     1741 ctcaagcgtt acgccgtacg agtgagtcct gctcgggagc ccggagtggt cagcgggtca
     1801 gctgctcgcg cagccaggcg atgtcctggg cgtggccggc cttgacgccg ggggtctcga
     1861 gcacggcgtc gcacccagcg gcggccacca cggcggccac cggctcggcg ccgatctcgc
     1921 cctcgcccag gtcggcgtgc cggtcgcgag ccgagccgaa cgggtcgcgc gagtcgttgg
     1981 cgtgcaccag gtcgacccgg ccggtcacgg cgcggaaccg ctcgacgacc tcggccagct
     2041 cgatgccggc cgagaacgcg tggcaggtgt ccaggcacag cccgacgtcg gtgtgcccga
     2101 cggcggccca cagccgctcg agctgctcga cggtgcgggc catcgcgccg ttgcccccgg
     2161 cggtgttctc gatgagcacc ggcgcaccgg gctcgagccg gtcgagggcc ttggcccagt
     2221 tggcgaagcc ggcggccgcg tcggctccgg aggtgacgtg cccgccgtgc acgatgaccc
     2281 cggcggcccc gatcgctccc gcggcggtga ccacctgctg gagcagcttg cggctcggga
     2341 tgcggatgcg gttgttggtc gtcgcgacgt tgatgacgta gggcgcgtgc acgtagaccg
     2401 cgatgtcggc ctcggccgcg gctcgtcgta cggcctcggc atcgacaccg tcgagcggcg
     2461 tggccttcca gtcctgcggg tcgctcacga agatctgcac gcagtcggca ccgcgcgagc
     2521 gcgcctcggc cacgaggtcg atctcgtcga tgcgcccgac gtgggcgccg acacgggtgc
     2581 gggcagggct tggcatgccg gcagcctagg gccgaccgcg acctaccaga ggcctccgtt
     2641 gtgcaggccg aacgccagcc cgaccgccga gccgatcagg gcgggtacga cgatcatccg
     2701 ctgggccgtg gtcgcggaca ggtactgcgc cacaccgccg gtgagcatcc cgatgagacc
     2761 ggcccagccg ccgagcaggt gccagctcgg cacgaacgcg caggtcagcg agatgatcgc
     2821 cgcgaccagg gtgatcgccg tcagcgtgtt ctcgcgcggg tgccggtgcc cgtcggtgtt
     2881 gagcgtcagg cgcggacgcg gatgcagcgt gtgggccatc gccccacctc cgaccgagat
     2941 ccgtcgtgcg accacggtgc gcgcgccgta cgccggattt caagggaaac ggctccgccc
     3001 gctaggctgg ccggactgcc tcgacgcagt agccctcctg ccacggagag accgtggccg
     3061 cgaagtccag aggaggtggg tcaagcatgc gtcgttatga gttgatggtg atcctcgatc
     3121 ccgatctcga ggagcgcacc gtcgccccca gcctcgacca gttcctcacc gtcgtccgca
     3181 acggcggcgg cagcgtggag aaggtcgacg tgtggggtcg ccggcggctg tcctacgaga
     3241 tcgccaagaa ggccgagggc atctacgcgg tggtcgacct gaccgccgag cccgacgtgg
     3301 tcaaggagct cgaccgccag ctcaacctca acgaggccgt gctgcgcacc aaggtcatgc
     3361 gccccgacaa gtaagccccg acgtcggctc cgtccggcac gtgaccgctg tcggacctcc
     3421 gcgctagcgt cgcgatcgac ccgacctcga gcgaagggaa cgccatggca ggcgagacca
     3481 ccatcacggt catcggcaac cttgtcgacg accccgagct gcggttcacg ccgtccggtg
     3541 cggccgtggc caagttccgg atcgcctcca ccccgcgcac cttcgaccgc cagaccaacg
     3601 agtggaaaga cggcgagagc ctcttcctca ccgtcaacat ctggcggcag gcagcggaaa
     3661 acgtcgccga gtcgctccag cgcggcatgc gggtgatcgt gtcgggccgg ctgcgtcagc
     3721 ggtcctacga gacccgtgag ggcgagaagc gcacggtcta cgagatcgag gccgacgagg
     3781 tcgggcccag cctgcgcaac gccaccgcca aggtcaccaa gacctcccgc ggcggcggcg
     3841 gcttcggcgg gtcggccggc gcgtccggcg gctcgtcggg tggcgcctcg ggcggcttcg
     3901 gctcctccgg cggcgacgac ccgtggtcga ccccggcgcc ctcctcgggc ggcggaggcg
     3961 gctggggcgg cggcagcacc gaccagcccc cgttctaacc cgttcatccc ccatcccgcc
     4021 cgcgtgagca gcgcggtcgg gctcgcacca gaagtggagc accacgatgg ccaagccccc
     4081 tgctcgcaag ccgaagaaga aggtttgcgt cttctgcaag gaaaagatca gccacgtcga
     4141 ctacaaggac acggcgttgc tgcgcaagta catctccgac cgcggcaaga tccgtgcccg
     4201 ccgcgtgacc ggcaactgca cccagcacca gcgtgacatc gcgacggcgg tcaagaacag
     4261 ccgcgagatg gcgctgctgc cctacacctc gaccgcgcgc tgaggctgag gagacgacat
     4321 gaagctcatc ctcacccagg aggtctccgg cctcggcgct cccggcgacg tcgtcgaggt
     4381 caaggacggc tacggccgca actacctcgt gccgcgcggg ctggcgatcc gctggacccg
     4441 gggcaccgag aagcagatcg cctcgatcaa gaaggcgcgt gaggtccgtg aggtccgcga
     4501 cctcgggcac gcccaggaga tcaagggtca gctcgaggcg ctcaaggtca agctgccggt
     4561 gcgcgccggg tcgggcggcc ggctgttcgg ctcggtgacc gtcgccgacc tcgtcgcggc
     4621 ggtcaagtcg gccggtggcc ccgacatcga caagcgcaag atcgaggtgg gcaacccgat
     4681 caagaccgtg ggcgcccaca ccgtcgccgt gcggatccac cccgaggtgg ccgccaccct
     4741 gcacgtcgag gtcgtcgccg gctgatcggc tgacctcctc accaccgccg aggcccggtt
     4801 ccccgctcac gtcggggcac cgggcctcgc cgcgtccccg cgtccccgcc gctcgctcgc
     4861 gctgcgattg accacgccaa gtgggcgtcg tggcactagg cgaggcgt
//
LOCUS       DATIBB010000066         2624 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_1609260, whole genome shotgun sequence.
ACCESSION   DATIBB010000066 DATIBB010000000
VERSION     DATIBB010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2624
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1609260"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..423
                     /locus_tag="VLC50_02360"
     CDS             <1..423
                     /locus_tag="VLC50_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013769831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlgN"
                     /protein_id="HSF26340.1"
                     /translation="LFKLEEEQLILASGRTRWLARATREVEMVLDQIRSAEVARAAEV
                     DAAAADLGLAPGPSLGALAQACSEPWDALLREHRAAFLQLTAEITALADTNREMLSAG
                     QRAVREAMLVISESSTTYSPTGTPVAAGAPRRLVDQAF"
     gene            428..1819
                     /gene="flgK"
                     /locus_tag="VLC50_02365"
     CDS             428..1819
                     /gene="flgK"
                     /locus_tag="VLC50_02365"
                     /inference="COORDINATES: protein motif:HMM:TIGR02492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HSF26341.1"
                     /translation="MSTFGSLSTALSALYAQQRGLDVTGQNIANANTEGYSRQRVDLK
                     SVGAPTVPAIWATYEGAGGGVTVSDVQRLRDMFLESRAHTEHGRLSYLQSMSTTLANV
                     EQTLGEPGETGLAAQLGEFWGYWHDVANNPGDLAARGQLLQQAGTITTTFHNAQRALA
                     AQWNAAREQVGTIADEVTATAARVAELNAAIARATQAGTPSNELADQRDLLAMRLADL
                     VGSTTKASTDGQVDVYLGGTALVRGATSNGLTVKGPGDFDSAGGAKVSLEWANGGYDA
                     AVTLGKAGALLESVNVAIPGYLADLDGVAKQLVEDVNTVHAAGYDLTGNTGINFFDPT
                     KLTADTIQVALSDPNQVAASAVPGGNLDGSNATKLADLGIGTTGPDATYRQLIVKLGV
                     QAQTTNQRVDIQASTTTNLDSAREAQAGVSLDEEMVNMMSFQHAYAAAARVMTTIDQT
                     LDVLINRTGLVGR"
     gene            1823..>2624
                     /gene="flgL"
                     /locus_tag="VLC50_02370"
     CDS             1823..>2624
                     /gene="flgL"
                     /locus_tag="VLC50_02370"
                     /inference="COORDINATES: protein motif:HMM:TIGR02550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HSF26342.1"
                     /translation="MSFRITQRSVAMRVLDGLQGNLERLGTTQQRLSSGKLVSKPSDS
                     PTAAVSSMQLRAGARAQAQYSRNATDGLGWLNTIDTALTSTLDNLRRVRDLTVQGMST
                     GSAGPDARAAIAKEVSQIRDAVIGLANTRYLDRPVFGGTTSKSAAFSSPGAFIGDPDT
                     TVARTIAPGTTVKVNINGEKIFADAFGNNGALTVVANIADHLVNDPAQLSTDLGNLDL
                     ALKNITEKLATVGASVNRLETARATADLNVDTLKTQLSDVEDIDLPKTL"
BASE COUNT          465 a          978 c          845 g          336 t
ORIGIN      
        1 ctgttcaagc tcgaggagga gcagctgatc ctcgcgtccg ggcgcacccg ctggctggcc
       61 cgggccaccc gcgaggtgga gatggtgctc gaccagatcc gctcggcgga ggtcgcgcgg
      121 gccgccgagg tcgatgcggc ggcggccgat ctgggcctgg cccccgggcc gagcctgggc
      181 gcgctggccc aggcctgctc cgagccgtgg gacgcgctgc tgcgcgagca ccgcgccgct
      241 tttctccagc tcaccgccga gatcaccgcg ctggccgaca ccaaccggga gatgctcagc
      301 gccggtcagc gcgccgtacg cgaggcgatg ctggtcatct ccgagagcag caccacctac
      361 tccccgaccg gcaccccggt agcggccggc gcgccgcggc ggcttgtgga ccaggcgttc
      421 tgacctgatg agcaccttcg gcagcctgtc gaccgccctg tcggcgcttt atgcccagca
      481 gcgcggcctt gacgtcaccg ggcagaacat cgccaacgcc aacaccgagg gctacagccg
      541 ccagcgggtc gacctcaagt cggtcggcgc gcccaccgtg ccggccatct gggccaccta
      601 cgagggcgcc ggcggcggag taaccgtctc ggacgtgcag cggctgcgcg acatgttcct
      661 cgagtcgcgc gcacacaccg agcatggccg gctgtcctac ctgcagagca tgtcgaccac
      721 gctggccaac gtcgagcaga ccctcggtga gcccggcgag acgggcctgg ccgcccagct
      781 cggcgagttc tggggctatt ggcacgacgt ggccaacaac cccggcgacc tggccgcgcg
      841 cgggcagctg ctgcagcagg ccggcaccat caccacgacc ttccacaacg cgcaacgcgc
      901 actggccgcg cagtggaacg ccgcccgcga gcaggtcggc accatcgccg acgaggtcac
      961 cgcgaccgca gctcgggtgg ccgagctcaa tgccgcgatc gcccgggcca cccaggccgg
     1021 cactccgtcc aatgagctag ccgaccagcg cgacctgctc gccatgcggt tggccgacct
     1081 ggtcgggtcg accaccaaag ccagcaccga cgggcaggtt gacgtctacc tcggcggcac
     1141 cgcactggtg cgcggcgcga ccagcaacgg cttgaccgtc aagggtccgg gcgacttcga
     1201 ctccgcaggg ggtgccaagg tctcgctgga gtgggccaac ggtggctacg acgcggccgt
     1261 aacgctgggc aaggccggtg cgctgctcga gtcggtcaac gtggcgatcc ccggctacct
     1321 cgccgacctt gacggcgtcg cgaagcagct cgtcgaggac gtcaacaccg tgcacgccgc
     1381 cggctatgac ctcaccggca acaccgggat caacttcttc gaccccacca agctcaccgc
     1441 cgacacgatc caggtcgccc tcagcgatcc caaccaggtc gccgcatccg cggtcccggg
     1501 cggcaatctc gacggcagca acgccaccaa gctcgccgac ctcggcatcg ggacgaccgg
     1561 gcccgatgcg acgtaccgtc agctgatcgt caagctcggc gtccaggcac agaccaccaa
     1621 ccagcgggtg gacatccagg ccagcacgac caccaacctc gactcggcgc gcgaggcgca
     1681 agccggcgtc agcctcgacg aagagatggt caacatgatg tcgttccagc acgcctacgc
     1741 cgccgcggct cgcgtgatga cgacgatcga ccagaccctc gacgtactca tcaaccgcac
     1801 tggcctggtc gggaggtaag ccgtgagctt tcgcatcacc cagcgctcgg tcgcgatgcg
     1861 cgtgctggac gggctgcagg gcaacctcga aaggctcggg acgacgcagc agcgactgtc
     1921 cagcggcaag ctggtgagca agccgtccga ctcgccgacc gccgcggtct cctcgatgca
     1981 gctgcgagcc ggggcgcggg cgcaggcgca gtattcccgc aacgccaccg acgggctggg
     2041 ctggctcaac accatcgaca ccgcgctgac cagcacgctc gacaacctgc gccgggtgcg
     2101 cgatctgacc gtgcaaggca tgagcaccgg atcggccggc cccgatgcgc gggcggcgat
     2161 cgccaaggag gtcagtcaga tccgcgacgc ggtgatcggg ctggccaaca cgcgctacct
     2221 cgaccgcccg gtcttcggcg gtacgacgag caagtcggcg gcgttcagct cccccggagc
     2281 attcatcgga gaccccgaca ccaccgtggc gcgaaccatc gcacccggca ccaccgtcaa
     2341 ggtcaacatc aacggcgaga agatcttcgc cgatgcattc ggtaacaacg gcgcgctcac
     2401 cgtggtcgct aacatcgccg accacctcgt gaacgatccc gcacagctca gcaccgacct
     2461 cggcaacctc gatctcgcgc tgaagaacat caccgagaag ttggccaccg tcggcgcgtc
     2521 ggtcaaccgg ctcgagaccg ctcgggccac cgccgacctc aacgtcgaca cgctcaagac
     2581 gcagctgtcc gacgtcgagg acatcgacct gcccaagacg ctga
//
LOCUS       DATIBB010000067         4977 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_426753, whole genome shotgun sequence.
ACCESSION   DATIBB010000067 DATIBB010000000
VERSION     DATIBB010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 4977)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4977)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4977
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_426753"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(3..1262)
                     /locus_tag="VLC50_02375"
     CDS             complement(3..1262)
                     /locus_tag="VLC50_02375"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSF26343.1"
                     /translation="MARIDEAVPSPDAVVSPAPTSISVPDYLRSTWARVKGGDTGVLP
                     VVAGLLLVFVLFQALNDRFLTAGNLVNLLVQAAVFSLLAMGQVFALLLGEIDLSIGYV
                     AGFGAVVMASLAEPSTGWPWWAAIGIALVLCALIGVLQGSLITRIGLPSFVVTLAGLL
                     FWQGIMLRILGDGGSILIQNDVINNISSGSMSRLAGWVLMLVIVGVFGVWTYRHDAGR
                     RAAGLVAPPPLLTLVKIAAALAGGIGLVLLCNTDRGRAVPIYGVPWVVVLVFTVLAVW
                     TYVLGRMRFGRYIYAIGGNAEAARRAGISLARIRTMAFVLCSLTAGIAGVVYASRLRS
                     ISTAIDGGTLVLYAVAAAVIGGTSLFGGRGKALHGVLGGLVIAAIDNGMGLLGFNAAS
                     KYIVTAIVLVVAVTIDALARRHRPAGR"
     gene            complement(1266..2081)
                     /locus_tag="VLC50_02380"
     CDS             complement(1266..2081)
                     /locus_tag="VLC50_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018784623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HSF26344.1"
                     /translation="MSADAGAEGATADESVSVLPGTPLLRLQSISKRFGAVRALTDVD
                     LDIPAGQVTSLAGDNGAGKSVLIKCIAGIHAPDAGQMWWNGRPVHVRSPRDAAALGIE
                     TVYQDLALCDNLDIVQNMFLGRERVRRTALNEESMEGAARETLTSLAVTTVRSIRQPV
                     ASLSGGQRQSVAIAKAVLWNSKLVIMDEPTAALGVAQTKMVLELVRRIADRGHAVLLV
                     SHNLNDVFEVADRVAVLHLGRMAAVRPITELDRQIVVDLMTTGRSTRIPSGGD"
     gene            complement(2115..3365)
                     /locus_tag="VLC50_02385"
     CDS             complement(2115..3365)
                     /locus_tag="VLC50_02385"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HSF26345.1"
                     /translation="MNISRKRTYVASLGMILSLGLVIAGCGDDGGSAGPTTGGATGAT
                     SGATGGTAASAPQVGVDDFTVDFAKMKELTDLAAKGDGLIGVLLPDTTTSTRYVQYDA
                     PYLKQAFEAAGLKSDQFKIDNAQGSASTMQTQAEADINAGASVLLVDALDPGSGAAIE
                     SKAAAAGVKVIDYDRLASGGPADRYYVSFDNVKVGSLIGQGMVDCLTAWKVATPNILV
                     MNGDPKDNNAKLFAQGYHSVLDPKFDDGSYTKAGEPAGTWDPQTAATTFQQQRTAHPD
                     INAAVTPNDDNANAVISALQSAGVKANTFPTTGQDASLPGLQNILKGYQCGTVYKPIY
                     VEAQAAAALALYLRAGETPPTTLVNAETDDTVLGKKVKSVYTTPIWVTTDNMAQTVVK
                     DKAVEVTKLCAGAVAPSCVKAAIQ"
     gene            3491..4321
                     /locus_tag="VLC50_02390"
     CDS             3491..4321
                     /locus_tag="VLC50_02390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF26346.1"
                     /translation="MALPLVVLVIAPVGLLVALTVALRRRPGRFDRHSTAPVLAGLLV
                     GAASAAAVWLADSPRHHLVLTLPAVAGLGWLAGVVVAERTRGHALGTARTASLLPRVP
                     GRYLGRHTLTLMRTTFALAAAVAVAGIVLAAPDGASFARLCADGSVSGHGPFPGARYA
                     VPALAALGLGWLGTEWALREVSRRPPVGPDEQADAAVRGYGARTAIVAAGLIAVPILG
                     GLLVPMGAGLHGACPGPTVSTVGAMLFWSGGLLILLSLLAWSAALASAGRAVVAVPRR
                     "
     gene            4318..4698
                     /locus_tag="VLC50_02395"
     CDS             4318..4698
                     /locus_tag="VLC50_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020668088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HSF26347.1"
                     /translation="MNITIDVASPVPPYEQLRAGIAALIRSGALPPGTPLPSVRQLAR
                     DLGLAPGTVQHGYVALREAGLVVSRSRRHVEVAPVAAVADARRAPGALEAAVSDIVAA
                     ARAAGLTDAELRALLDRALEAADH"
     gene            complement(4750..4953)
                     /locus_tag="VLC50_02400"
                     /pseudo
     CDS             complement(4750..4953)
                     /locus_tag="VLC50_02400"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
BASE COUNT          727 a         1769 c         1759 g          722 t
ORIGIN      
        1 agctagcgtc cagcggggcg gtggcgccga gccagggcgt cgatcgtgac cgctacgacg
       61 agcacgatcg cggtgacgat gtacttggaa gccgcgttga accccagcag tcccatgcca
      121 ttgtcgatgg ccgcgatgac gaggccgccg agcacaccgt gcaacgcctt gccacggcct
      181 ccgaacaggc tggtgccgcc gatgacggcg gccgccaccg cgtagagcac cagggtgccc
      241 ccgtcgatcg cggtcgagat cgagcgcagc cgggaggcgt agaccacgcc cgcgatgccg
      301 gcggtgagcg agcacagcac gaacgccatg gtgcggatcc gagccaggct gatgccggcg
      361 cgacgggccg cctcggcgtt gccgccgatg gcgtagatgt agcggccgaa ccgcatccgc
      421 cccagcacgt aggtccacac ggccaggacg gtgaagacga ggaccacaac ccatggcacg
      481 ccgtagatcg gaacggcgcg gccgcggtcg gtgttgcaca gcagcaccag cccgatgcca
      541 ccggcgagcg ccgcggcgat cttcacaagg gtgagcagcg gcggcggcgc gaccagcccg
      601 gccgcgcgac ggccggcgtc gtggcgatag gtccacaccc cgaagacccc gacgatgacc
      661 agcatcagca cccagccagc cagccgcgac atgctgccgc tggagatgtt gttgatcacg
      721 tcgttctgga tgaggatcga accaccgtcg ccgaggatcc gcagcatgat gccctgccag
      781 aacagcagac cggccagcgt caccacgaac gacggcagcc cgatccgcgt gatcagcgag
      841 ccctgcagga cgccgatgag ggcacacagc accagcgcga tgccgatcgc ggcccaccac
      901 ggccagccgg tcgagggctc tgccaggctg gccatcacca ccgcgccaaa gcccgcgaca
      961 tagccgatcg acaggtcgat ctcaccgagc agcagcgcga agacctggcc catcgccagc
     1021 aggctgaaca ccgccgcctg caccagaagg ttgaccaggt tgccggcagt gaggaaccgg
     1081 tcgttgaggg cctggaacag cacgaagacg agcaacaacc cagcgacgac gggaagcacc
     1141 ccggtgtcgc cacccttgac ccgtgcccag gtcgagcgca ggtagtcggg aaccgagatc
     1201 gacgtcggcg cgggcgagac caccgcgtca ggggagggga ccgcctcgtc gatgcgcgcc
     1261 atcggctagt caccccctga ggggatgcgg gtggaacgcc cggtggtcat caggtccacg
     1321 acgatctgcc ggtcgagctc ggtgatggga cgtacggcgg ccatccggcc caggtgcagc
     1381 acggcgaccc ggtcggcgac ctcgaagacg tcgttgagat tgtgcgagac caggagcacg
     1441 gcgtggccgc ggtcggcgat ccggcggacc agctcgagca ccatcttggt ctgcgcaacc
     1501 ccgagcgccg cggtcggctc gtccatgatc acgagcttgg agttccacag caccgccttg
     1561 gcgatggcca ccgactgacg ctgcccaccg gacagcgagg ccaccggctg gcggatcgac
     1621 ctgaccgtgg tgaccgccag gctcgtaagc gtctcgcggg ccgccccctc catcgactcc
     1681 tcgttcaatg cggtacgacg cacgcgctcc cggcccagga acatgttctg gacgatgtcg
     1741 aggttgtcgc acagcgcaag gtcctggtaa acggtctcga tgcccagtgc ggccgcgtcg
     1801 cgcgggctgc ggacgtgcac cggccggccg ttccaccaca tctgcccggc atcgggggca
     1861 tgaatgccgg cgatgcactt gatgagcacc gacttgccag cgccattgtc gccggccagc
     1921 gaggtcacct gacccgccgg gatgtcgaga tcgacatcgg tcagcgcgcg tacggcgccg
     1981 aagcgcttgc tgatgctctg aaggcgcagc agtggggtcc ccggcagcac cgagaccgac
     2041 tcgtcggcgg tggcgccctc cgcacccgcg tctgcactca tgtcgcccca ccttccacag
     2101 cccgatgggt ccgactactg gatggccgcc ttgacgcagg acggcgcgac cgcgccggcg
     2161 cacagcttgg tcacctcgac cgccttgtcc ttgaccacgg tctgagccat gttgtcggtg
     2221 gtgacccaga tcggcgtggt gtagacggac ttgaccttct tgccgagcac ggtgtcgtcg
     2281 gtctcggcgt tgaccagggt ggtcggcggt gtctcaccgg cgcgcaggta gagcgccaac
     2341 gcggcggcgg cctgtgcctc gacgtagatc ggcttgtaga ccgtgccgca ctggtagccc
     2401 ttgaggatgt tctgcaggcc cggcagcgac gcgtcctgcc cggtggtcgg gaacgtgttg
     2461 gccttcaccc cggccgactg cagcgcagag atcacggcgt tggcgttgtc gtcgttgggt
     2521 gtcacggccg cgttgatgtc ggggtgcgcg gtgcgctgct gctggaacgt ggtcgccgcg
     2581 gtctgcgggt cccaggtacc ggcgggctcg ccggccttgg tgtagctgcc gtcatcgaac
     2641 ttcgggtcga gcaccgagtg gtagccctgc gcgaacagct tggcgttgtt gtccttgggg
     2701 tcgccgttca tcacgaggat gttgggcgtg gccaccttcc aggcggtgag gcagtcgacc
     2761 atgccctggc caatcagcga gccgaccttg acgttgtcga agctgacgta ataacgatcc
     2821 gccggcccgc ccgaggccag ccggtcgtag tcgatcacct tgacgccggc ggccgcggct
     2881 ttggactcga tcgccgcacc gctgcccggg tcgagggcat cgaccagcag cacggaggcc
     2941 ccggcgttga tgtctgcctc ggcctgggtc tgcatcgtcg acgcgctgcc ctgcgcatta
     3001 tcgatcttga actggtcgga cttgagcccg gccgcctcga acgcctgctt gaggtagggc
     3061 gcgtcgtact gcacataacg ggtcgaggtg gtggtgtcgg gcagcagtac gccgatcagc
     3121 ccgtcgcctt tagccgccag gtcggtcaac tctttcatct tggcgaagtc gacggtgaag
     3181 tcgtcgacac ccacctgcgg cgccgacgcc gccgtcccgc ccgtggcgcc cgacgtggcg
     3241 ccggtcgcgc cacccgtggt tggcccggcc gacccgccgt cgtcaccgca tccagcgatc
     3301 accagcccca ggctgaggat catgcccagc gacgcgacgt aggtgcgctt gcgagagatg
     3361 ttcacgatgg caacctcgat ccgcagggga cccccgtccc tgcctcactc gcatcgtgcg
     3421 cccgtcgggg cgcgtttggg acctgcttcc gccgaaaaca tctgtatcga ctagttgata
     3481 cagtcggctc atggcgctcc cgctggtcgt cctggtgatc gcgcccgtcg gcctgctggt
     3541 cgcgctcacg gtggcgctgc gtcgacgacc cggccggttc gaccgccact ccaccgcacc
     3601 ggtgctggct gggctgctcg tcggggccgc gtcggccgcc gcggtgtggc tggccgactc
     3661 gccgcggcac cacctggtcc tcaccctgcc cgccgtggcc gggctcggct ggctcgccgg
     3721 cgtggtggtg gccgagcgga ctcgcggtca cgccctcggc accgcgcgga ccgccagcct
     3781 cctgccgcgg gtgcccgggc gctacctcgg ccggcacacc ctcaccctaa tgcgtacgac
     3841 gttcgcgttg gccgccgccg tcgcggtcgc cggcatcgtc ctggcggcgc cggatggcgc
     3901 gagcttcgcc cggctctgcg ccgacggctc ggtgtcgggg cacgggccgt tccccggtgc
     3961 ccgctatgcc gttccggcgc tggcggcgct cgggctgggc tggctgggca ccgagtgggc
     4021 gctgcgcgag gtcagccgcc ggccaccggt cggccccgac gagcaggccg atgcggcggt
     4081 gcgcggctac ggcgcccgta cggcgatcgt ggccgccggc ctcatcgccg taccgatcct
     4141 tggtgggctg ttggtgccga tgggcgccgg gctgcacggc gcctgccccg gccccacggt
     4201 cagcacggtc ggcgcgatgc tcttctggtc gggtgggctg ctgatcctgc tctcgctgct
     4261 ggcgtggtcg gcggcgctgg cgagcgccgg gcgcgccgtg gtcgcggtgc cccgacggtg
     4321 aacatcacca tcgacgtagc ctccccggtc ccgccctatg agcagctgcg cgccggcatc
     4381 gccgcgctca tccgctccgg ggcgctgccg ccgggcaccc cgctgccctc ggtccgtcag
     4441 ctcgcccgcg acctgggcct agcaccgggc acggtgcagc acgggtacgt cgcgctgcgc
     4501 gaggcgggcc tcgtggtcag ccgctcgcgc cggcacgtcg aggtcgcccc cgtcgccgcc
     4561 gtggccgacg cccggcgcgc accgggcgcg ctcgaggccg cggtgagcga catcgtggcc
     4621 gctgcccgcg cggcggggct gaccgacgcc gagctgcgcg cgctgctgga ccgcgcgctc
     4681 gaggcggccg accactagcg ggacgcgcag ccggggcggg ctggcctggg cggcggtgat
     4741 ggccccgagc gcgaggacga atccgaggcc ctgcaaggag gtcagcgtcg ggtcgagcaa
     4801 cgcccagccg agcaaggtgg ccacgaccgg ggacagcagc ggcaagaacg agaacgcgcc
     4861 gaccggcagc ttggccaggc cgcggaacca caagacgtaa gccagcaggg tgccgacgag
     4921 cgcgagccag gcgtagccga ggacggcgtc cacgcccagc gccgggggag ccccctc
//
LOCUS       DATIBB010000068         2399 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_1084660, whole genome shotgun sequence.
ACCESSION   DATIBB010000068 DATIBB010000000
VERSION     DATIBB010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2399
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1084660"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(3..1364)
                     /gene="hisS"
                     /locus_tag="VLC50_02405"
     CDS             complement(3..1364)
                     /gene="hisS"
                     /locus_tag="VLC50_02405"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011755843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HSF26348.1"
                     /translation="MARPTPLSGFPEWLPAQRVVEAEVIERLRRTFELHGFAGIETRA
                     VEPLEQMLRKGEIDKEVYLLRRLHADPDEAETAGSALGLHFDLTVPFARYVLEHAGKL
                     TFPFRRYQIQKVWRGERPQEGRYREFVQADIDVVDVDTLAPQHDAEMARVMAEALSAL
                     PLPAFRLQVNNRKLIEGFYRGLGIDQVDVPAVMRVVDKLDKVGQPGVRAMLTDDVGLD
                     DKRTSAALALAAIASADPRAVEAAVAELGVSDPLLDEGLAELVAVLEATASVRSDRVA
                     VVADLAIARGLDYYTGTVYETRMAGFESLGSICSGGRYDALASDGRTTYPGVGISFGV
                     TRALAPLFARGLLTASRPTPTAVVVAVVDEAHRAASDAVATALRARGIACEVSPTAAK
                     FGKQIRFADRRGIPFVWFPAGATESGVDEVKDIRSGDQAPADPATWTPPAEDLRPHIV
                     TTA"
     gene            complement(1369..2052)
                     /locus_tag="VLC50_02410"
     CDS             complement(1369..2052)
                     /locus_tag="VLC50_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006591370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HSF26349.1"
                     /translation="MLVLGFPAAAFATNCYVLAPAAGEECVVVDPGIGVVDRLAEVLA
                     EHRLRPAAVLLTHGHVDHVYSVTPVCSTHGVAAYLHGDDRYRLANPLALLDPALLTML
                     ENQFGRTATWAEPDDVVTLADGAALEVAGIPLTVDHAPGHTEGSVLFRIAAGTHGTET
                     PLCLSGDVLFAGSIGRTDLAGGNHAAMLASLATKVLPLPDETVVLPGHGPQTTIGRER
                     VTNPYLQGV"
BASE COUNT          325 a          853 c          909 g          312 t
ORIGIN      
        1 cgtcaggcgg tggtgacgat atggggacgc agatcctcgg cgggtggggt ccaggtggcc
       61 gggtcggccg gggcctggtc gccggagcgg atgtccttga cctcgtcgac gccgctctcg
      121 gtggcgccgg ccgggaacca cacgaacggg atgccgcgcc ggtcggcgaa gcggatctgc
      181 ttgccgaact tcgccgcggt cggcgacacc tcgcaggcga tgccacgagc ccgcaacgcc
      241 gtggccaccg cgtcggaggc ggcccggtgg gcctcgtcga ccaccgcaac cacgacggcg
      301 gtcggggtcg gccggctggc ggtgagcagc ccgcgggcaa acagcggcgc cagcgctcgg
      361 gtcaccccga acgaaatgcc cacccccggg tacgtcgtac gcccgtcact ggccagtgcg
      421 tcgtagcgcc cgccggagca gatcgacccg agcgactcga agccggccat ccgggtctcg
      481 tagaccgtgc cggtgtagta gtcgagcccg cgggcgatcg ccaggtcggc gaccaccgcg
      541 acccggtcgc tgcgcaccga cgcggtggcc tcgagcaccg cgaccagctc ggccagcccc
      601 tcgtcgagca gcgggtcgct cacgccgagc tcggccaccg ccgcctcgac ggcgcgcggg
      661 tccgcgctcg cgatcgcggc cagcgccagc gcggcgctcg tgcgcttgtc gtcgaggccg
      721 acgtcgtcgg tcagcatcgc ccggaccccg ggctggccga ccttgtcgag cttgtcgacc
      781 acccgcatca ccgccggcac gtcgacctgg tcgatgccca gcccgcggta gaagccctcg
      841 atcagcttgc ggttgttgac ctgcagccgg aacgccggca gcggcagcgc cgacagcgcc
      901 tcggccatca cccgggccat ctcggcgtcg tgctgcgggg ccagcgtgtc cacgtcgacc
      961 acgtcgatgt cggcctggac gaactcgcgg tagcggccct cctgcggccg ctcgccgcgc
     1021 cagaccttct ggatctggta gcggcggaac gggaaggtca gcttgccggc gtgctcgagc
     1081 acgtagcggg cgaacggcac ggtcaggtcg aagtgcagcc ccagcgccga gccggcggtc
     1141 tcggcctcgt cggggtcggc gtgcagccgg cgcaggaggt agacctcctt gtcgatctcg
     1201 cccttgcgca gcatctgctc gagcggctcg accgcacggg tctcgatgcc ggcgaagccg
     1261 tgcagctcaa acgtacggcg cagccgctcg atgacctcgg cctcgacgac ccgctgcgcg
     1321 ggcagccact cgggaaaccc cgacaacggg gtgggacggg ccatcggttc agactccctg
     1381 cagataaggg ttggtgacgc gttcgcgccc gatcgtggtc tgcgggccat gcccaggcag
     1441 tacgacggtc tcgtcgggca gcggcagcac cttggtggcc aggctggcca gcatcgcggc
     1501 gtggttgcca ccggcgaggt cggtgcgccc gatgctgccg gcgaagagca cgtcgccgga
     1561 caggcacagc ggcgtctcgg tgccgtgggt gccggcggcg atgcggaaga gcaccgagcc
     1621 ctcggtgtgc ccgggcgcgt ggtcgacggt cagcgggatg ccggcaacct cgagggcggc
     1681 cccgtcggcg agcgtcacga cgtcgtcggg ctcggcccag gtcgccgtac gcccgaactg
     1741 gttctcgagc atcgtcagca gcgcggggtc gagcagcgcc aacgggttgg ccagccggta
     1801 gcggtcgtcg ccgtgcagat aggcggccac gccgtgggtg gagcacacgg gagtcaccga
     1861 gtagacgtgg tcgacgtgcc cgtgggtgag caggacggcc gccggccgca gccggtgctc
     1921 ggcgagcacc tcggcgagcc ggtcgaccac gccgatcccg gggtcgacga ccacgcactc
     1981 ctcgccggcg gcgggcgcca ggacgtagca gttggtcgcg aacgccgcgg cggggaatcc
     2041 gaggacgagc acgggggtcc ttccgttggg cggccgtccc gagggtaccg gccgggcacg
     2101 cacccggatc gtgtgcccga cggcgggtcg catggaccgc ccctgcccgg cggctgccta
     2161 gactgaccgg gctaccgacc ggggacccgc tggtggccgg gtctccccat gatcaccacc
     2221 aggccggatc ggaggacgcg agtggccgcg agcagcaagc gggccaagga gctggcccgt
     2281 gcgcgctacg agcgcgccca gcagcgggag gctgcccgcg cccgtcgacg gcgccggtcc
     2341 acggtggtcg ccgccggggc gtcggtgctg gtcgtcgtac tcgtcgtgtg gggggtggc
//
LOCUS       DATIBB010000069        13713 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_954274, whole genome shotgun sequence.
ACCESSION   DATIBB010000069 DATIBB010000000
VERSION     DATIBB010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 13713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13713
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_954274"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1259
                     /locus_tag="VLC50_02415"
     CDS             <1..1259
                     /locus_tag="VLC50_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018550110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HSF26350.1"
                     /translation="FDPVRKQYFWHRFFTHQPDLNYDNPAVGQAVLEALRFWLDLGID
                     GFRLDAVPYLFEREGTNCENLAETHDFLKTVRKVVDDEYPDRVLLCEANQWPSDVVEY
                     FGDPETGGDECHMAFHFPVMPRIFMAVRRESRYPISEILAQTPLIPESCQWGIFLRNH
                     DELTLEMVTADERDYMWAEYAKDPRMKANIGIRRRLAPLLDKDANQIELFNALLLSLP
                     GSPVLYYGDEIGMGDNIWLGDRDGVRTPMQWTPDRNAGFSTCDPGRLTLPIIMDAVYG
                     YQVTNVEAQLQSTSSLLHWMRRMIQVRKQNPAFGMGTFHDIGGSNPSVLSYVREFGDD
                     VVLCVNNLSRFAQPVELDLRRYEGVEPIELLGSVRFPPIGELPYLLTLGGHGFYWFRL
                     RRSSTEPDTGDTQGHEAARAETGGSP"
     gene            1256..2668
                     /locus_tag="VLC50_02420"
     CDS             1256..2668
                     /locus_tag="VLC50_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018503731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase"
                     /protein_id="HSF26351.1"
                     /translation="MSVPTTPRLDAGVRATLEDALAGWLPIQRWFSGKDRPILSVHVL
                     ADTPLSTEGAVAELHHLVVAVAQAGSTDVYQVPVSLRHELDDRLEHALIVEVDGRLVH
                     DALHDKEATATLLRGFAVGQVDTLEFRAEPGAELPYDEPSIVMSVEQSNTSLVYGDAA
                     ILKVFRRLSPGRNPDIEIHDALTRAGCPYIAELLGWIEGRWPREVGTVEATELGSMAM
                     LQRFLVTAADGWDLAKTSVRDLYAEGDLHADEVGGDFAGEARRLGAATAQVHATMRAT
                     LATGTLDDAEVGARVEAMRTRLQRALDVVAELRPYAPVLHKTYDALADLGGPVPVQRV
                     HGDFHLGQVVRTVSGWKLLDFEGEPTRPLAERGGLDSPLRDVAGMLRSFDYAARHLLN
                     EHPGGEAQLSYRAGEWSARNRGAFCEGYAEVAGRDPREDEVLLRAYETDKAVYEAVYE
                     AQNRPAWLAIPLAAVERLAT"
     gene            2668..4932
                     /gene="glgB"
                     /locus_tag="VLC50_02425"
     CDS             2668..4932
                     /gene="glgB"
                     /locus_tag="VLC50_02425"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010232010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HSF26352.1"
                     /translation="MTATERPVERATLEALVGGDLADPHAVLGAHPHEGAVTVRVLRP
                     WASRVAVTYADQRVPLRHEHDGVWVGTLPVPEVPDYRLEVAYGGEPGPADDPYRFLPT
                     LGELDLHLLAEGRHEQLWSVLGAHVRRFDSPAGPVTGTSFAVWAPNARGVRVVGDFNY
                     WDGRAHPMRSLGASGVWELFVPGVEAGSTYKFAILDRDGHWHTKADPVAFATEAPPAT
                     ASVVYTSTYEWHDHDWLARRAAHDPQTGPMSVYEVHLGSWRPGLGYRELAGELVDYVR
                     EQGFTHIELLPVAEHPYGGSWGYQVTSYFAPTARYGSPDDFRYLVDQCHQAGIGVILD
                     WVPAHFPRDSWALARFDGTALYEHPDPRRGEQRDWGTLVFDFGRREVRNFLVANALFW
                     LEECHIDGLRVDAVASMLYLDYSRNEGEWVPNRFGGRENLEAVAFLQEMNATVYKRVP
                     GVITIAEESTAWPGVTRPTHLGGLGFGLKWNLGWMHDSLAYLRHEPVHRQFHHHEMTF
                     SMMYAFTEHFVLPLSHDEVVHGKGSLLRKMPGDRWQQLATLRAYLAFMWAHPGKQLVF
                     MGAELGQEAEWSEARSLDWWLLEHPDHAGVQTLVRDLNRVYRQTLALWSLDHEPDGFS
                     WIDANDSGNNTFSFLRHGTDGSVLACIANFAAVPHEGYRLGLPAAGRWDELLNTDAEV
                     YAGSGVGNLGRVEAEPVPWHGQPASATLRVPPLGTLWLRYAGSSRTTTALETGVLLPL
                     AVESSGTSSLASGK"
     gene            complement(4830..7118)
                     /locus_tag="VLC50_02430"
     CDS             complement(4830..7118)
                     /locus_tag="VLC50_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007273138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FdhF/YdeP family oxidoreductase"
                     /protein_id="HSF26353.1"
                     /translation="MTRKPLVEDVTDEHLGVRRPATAAAGLPGVRHSVVPAIREMGLG
                     RAVVSASKMNQKDGFDCMSCAWPDPDHRKKVEFCENGLKALTWEATPLTVPPDFWAEH
                     SVTDLRGRSEYWLGQQGRLVDPVYKPADDDHYRRITWAEAIGIVADKLRALESPDEAA
                     FYTSGRTANETAFLYQLFVRAFGTNNLPDCSNMCHESTGLAMSQSIGIGKNTISYADF
                     AKADLIIIMGQNPGTNHPRMLTALEEAKAAGAQIVAINPLPETGLIRFKNPQRVSGLL
                     GRGTPLADQFVQIRLGGDMALLQAVAKRVFEAEAAHPGTVLDQEFLAEHCEGLDAYRR
                     HIGALDEEAVTRATGVQAAEIDELAARYIKADKVIITWAMGLTQHRAAVPTIREVINL
                     LLLRGNIGRPGAGAAPIRGHSNVQGDRTMGIWEQMPKRFLDALAAEFGFEPPREHGVD
                     SVRAIRGMRDGRIKVWVALGGNLVSAISDTAVAEAALAATELTVQISTKLNRSHAVVG
                     REALILPTLGRSEIDLQATGPQFVSVEDSVCAVHASQGVLEPASPNLLSEISIITRLA
                     RAVLGERVAVDWAGFERDYDLIREHIARVVPGFDDFNAKVRREGGFVLPHGPRDSRTF
                     PTPSGKAVIAVNELEHVECPPGRLLLQTVRSHDQFNTTIYGLNDRYRGVRKGRRVVFV
                     NPDDLAELGVRDGTLVDVHSEWADGVDRVVRGFRVIAFPTARGCAAAYFPEANELVPL
                     DSTAKGSNTPVSKAVVVRLEPA"
     gene            complement(7193..8062)
                     /locus_tag="VLC50_02435"
     CDS             complement(7193..8062)
                     /locus_tag="VLC50_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020554035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF26354.1"
                     /translation="MTPRIALATAAAYPDLAADGPLLLDALARHEVAAEPVVWTDPHA
                     RWSSYDLVLVRSTWDYFHRRDEFLAWIERVAGVTRLANPPEVLRWNTDKTYLRDLAAA
                     GAPVVPTTWLHPGDTFVAPAGGDYVVKPAVSAGSLDTARYRSGEHDRLADEHVGRLLA
                     AGRTVMVQPYLEAVDTAGETAQLFFAGRFSHAIRKAPMLGTAGGPPGKEYIEARVPSA
                     AERAAAEDVLDRLPCPRASLTYARVDLVPGDDGTPRLMELEVTEPSMFLGHDPGAADR
                     FARAVVATLGQAS"
     gene            complement(8094..9476)
                     /locus_tag="VLC50_02440"
     CDS             complement(8094..9476)
                     /locus_tag="VLC50_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HSF26355.1"
                     /translation="MASTRTIDESFDELAATVRGSVLRPDSPGWPEEVAAYNLAVAHT
                     PRLVVVAADAQDVAAAVRYAGAHALPVAVQATGHGAEVAIDGGLLVSTRALDSVTIDP
                     ARRTATVGAGVRWRAVLDAAAPHGLAPLSGSTSDVSVVGYTLGGGLPILGRTFGFSAD
                     HVVSLELVTADGRVRQVDAVSEPDLFWALRGGKGNLGIVTEMTVELVEVDTVFGGGIF
                     YAAEHIPTLLHAFRTWVPDLPEHTSASIAVMRVPPFPEVPEPLRGQTVAHLRFCHLGD
                     VAEGEALLAPMRAAAPAMMDAVGPMRFVEADSIHQDPDHPVPFVEQGTLLRELTEDTV
                     EAVLGVAGPQVETPLLMCEIRLLGGALARPAAVPNAVGGRDAAFGVETVAIPMGPAPE
                     AALAAVESTVAALAPWSTGRTMVNFHGVPGDEADRARAWDEPTYQRLCALVRELDPAG
                     MFRFGHAIGR"
     gene            9633..11000
                     /locus_tag="VLC50_02445"
     CDS             9633..11000
                     /locus_tag="VLC50_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010592840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HSF26356.1"
                     /translation="MVTTGARPIGPVDAIWLNMDRPNNLMVIDALLWLHEPITRARLG
                     EVVRQRLVERYPVFRQRPVPGGAPSASGWTRRHWEEDPEFALDRHVRRARLRPPGDDA
                     ALHHFVDARLSRPFDRRHPLWEIHLIDGYGSGAVVLARMHHALADGVALAQVLLSLTD
                     ASVGSDGGVADPPRPAAPARGPADTVAAGISLVRRYATPRGLVEVLALARQAGGVVGK
                     LVLGSNPATPLSGRPGVAKHAVWSAPRPLQPVKTLGKMTGATVNDVLVCAVAGGLSRY
                     LAEHGSPPVDLTTMVPVNLRPQGVPLPAELGNRFALVLLELPSGRWPVLARLAETKRR
                     MDAIKHSPEAAITFGLISAIGRATPYVDQPLVDFFSSKAFGVTTNVAGPTTERSLAGS
                     QVDSVLGWVPGSGDQGVGVSIVTYNGTVRLGFKVDAVIVPDPDRLVAAAEASLDELLR
                     ITRNA"
     gene            11017..11469
                     /locus_tag="VLC50_02450"
     CDS             11017..11469
                     /locus_tag="VLC50_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003937283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HSF26357.1"
                     /translation="MSTDVATDTIEVDAPPAQVLATLRDVAAQPHWVREVTSVDLLDT
                     DEQGRPATARVTATTPMGTDEYTLAYEHTDEGMSWSMVEGRLQTGQDGHYTLEGLDDD
                     RTRVTFELAISHNLPLPGFLTRQVVNGVVTGTLAGLKRYVEEPAGSTH"
     gene            complement(11504..12475)
                     /locus_tag="VLC50_02455"
     CDS             complement(11504..12475)
                     /locus_tag="VLC50_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003950931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSF26358.1"
                     /translation="MSTPMTPRGSAGPAINVRGAVDLAAVAAANERRAAAEAASASGA
                     NAVSSLVVDVTEATFQAEIIERSQTVPVVIDFWAEWCGPCKQLSPVLEKLVEEYDGRW
                     LLAKVDVDAEQRLGAAFGVQSIPTLVAVVGGQMVPLFQGAVPEPQVRQVLDELLRVAE
                     ANGVSGRLTRGEPADGETAVEPAEPALPPDLQAAEDAMLAGDYDGAATAYRAVLATRP
                     ADEVARLGLARVELLARTQDVDPAAARTAAAENPADVAAQIVAADLDLLGGHVEDAFA
                     RLIDTVRATSGEDRNTAREHLIALFDVVGPEDPRVGPARLALANALF"
     gene            complement(12530..>13713)
                     /locus_tag="VLC50_02460"
     CDS             complement(12530..>13713)
                     /locus_tag="VLC50_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020513197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HSF26359.1"
                     /translation="ALAMYLVVGEEQGVPRDRLRGTIQTDILKEYIAQKEYIYPPEPS
                     MRLCVDMVEYCARELPRMHPISISGYHIREAGSTAQQELAFTLADGFAYVQAAVERGL
                     AVDDFAPRLSFFFNAHIDFFEEIAKYRAARRIWAREMRDTYGARDPASWRMRFHSQTA
                     GVSLTAQQPNNNIVRTAVEALAAVLGGTNSLHTNALDEVLALPSEKAAEIALRTQQVL
                     MEETGVVNVADPLGGSWYVEELTDRLEAEAEAIFARIRDMGGDGTITSGLLRGIEDGW
                     FMAEIAEAAFRYQQALEKGEKKVVGVNSHTSTVEEPLEILRVSHEVEVEQNRALGQRR
                     TARDQAGVDAALARMVEVARGEGNTVPPMLEAARAEATLGEICGALRQVWGDYREPAR
                     F"
BASE COUNT         1923 a         5019 c         4842 g         1929 t
ORIGIN      
        1 ccttcgaccc ggtgcgcaag cagtatttct ggcaccgctt cttcacccac cagcccgacc
       61 tcaactacga caacccggcg gtgggccagg cggtgctcga ggcgctgcgg ttctggctcg
      121 acctgggcat cgacggcttc cgcctcgacg cggtgcccta cctgttcgag cgcgagggca
      181 ccaactgcga gaacctcgcc gagacccatg acttcctcaa gaccgtgcgc aaggtcgtcg
      241 acgacgaata ccccgaccgg gtgctgctgt gcgaggccaa ccagtggccc tccgacgtcg
      301 tggagtattt cggcgacccg gagaccggcg gcgacgagtg ccacatggcc ttccacttcc
      361 cggtgatgcc gcgcatcttc atggccgtac gacgcgagtc gcgctacccg atctccgaga
      421 tcctggccca gacgccgttg atccccgaga gctgccagtg gggcatcttc ctgcgcaacc
      481 acgacgagct gaccctcgag atggtcaccg ccgacgagcg cgactacatg tgggccgaat
      541 acgccaagga cccgcggatg aaggccaaca tcggcatccg ccgccggctg gccccgctgc
      601 tggacaagga cgccaaccag atcgagctgt tcaacgcgct gctgctgtcg ctgcccggct
      661 cgccggtgct ctactacggc gacgagatcg gcatgggcga caacatctgg ctcggtgacc
      721 gcgacggggt gcgcaccccg atgcagtgga cccccgaccg caacgccggc ttctccacgt
      781 gcgacccggg ccggctcacc ctgccgatca tcatggacgc ggtctacggc taccaggtga
      841 ccaacgtcga ggcccagctg cagagcacct cgtcgctgct gcactggatg cggcggatga
      901 tccaggtgcg caagcagaac cccgccttcg ggatgggcac gttccacgac atcggcggca
      961 gcaacccgag cgtgctgtcc tacgtgcgcg agttcggcga cgacgtcgtg ctctgcgtca
     1021 acaacctctc gcgcttcgcc caaccggtcg agctcgacct gcgtcgctac gagggggtcg
     1081 agccgatcga gctgctgggc agcgtccggt tccccccgat cggggagctg ccctacctgc
     1141 tcaccctcgg cgggcacggg ttctactggt tccggctgcg ccgatcgagc acggagcccg
     1201 acactggcga cacccagggg cacgaggccg cccgagccga gaccgggggg tccccatgag
     1261 cgtcccgacc acacctcgac tcgatgccgg ggtgcgggcc acgctcgagg acgcgctcgc
     1321 gggctggctg ccgatccagc gctggttctc cggaaaggac cgcccgatcc tgtcggtgca
     1381 cgtgctcgcc gacacgccgc tctccaccga gggagccgtc gccgagctgc accacctggt
     1441 ggtcgcggtc gcccaggcgg gcagcaccga cgtctaccag gtgccggtgt cgctgcgcca
     1501 cgagctcgac gaccgtctcg agcacgccct gatcgtcgag gtggacggac ggctggtcca
     1561 cgacgcgctg cacgacaagg aggcgaccgc cacgctgctg cgcggcttcg ccgtcgggca
     1621 ggtcgacacg ttggagttcc gggccgagcc cggcgccgag ctgccctacg acgagccgtc
     1681 gatcgtgatg tcggtcgagc agagcaacac gtcgctggtc tatggcgacg cggcgatcct
     1741 caaggtgttc cggcggctct ccccggggcg caaccccgac atcgagatcc acgacgcgct
     1801 gacccgcgcc gggtgcccct acatcgccga gctgctcggc tggatcgagg gacgctggcc
     1861 gcgtgaggtc ggcacggtgg aggccaccga gctgggcagc atggcgatgc tccagcgctt
     1921 cctcgtcacc gccgccgacg gatgggacct ggccaagacc agcgtgcgcg acctctatgc
     1981 cgagggtgac ctgcacgccg acgaggtggg cggcgacttc gccggcgagg cgcgccggct
     2041 gggcgcggcc accgcccagg tgcacgccac gatgcgcgca acgctggcca ccggcacgct
     2101 cgacgacgcg gaggtcggcg cccgggtcga ggcgatgcgc acccggctgc agcgggcgct
     2161 cgacgtcgtg gccgagctgc gcccctatgc cccggtcctg cacaagacct acgacgcgct
     2221 ggccgacctc ggcggcccgg tgccggtcca gcgcgtgcac ggcgacttcc atctcgggca
     2281 ggtcgtgcgc acggtgtcgg gctggaagct gctcgacttc gagggcgagc cgacccggcc
     2341 gctggccgag cggggcgggc tcgactcccc gctgcgtgac gtcgccggta tgctgcgctc
     2401 gttcgactac gccgcccggc acctgctcaa cgagcacccg ggaggcgagg cccagctctc
     2461 ctaccgcgcc ggcgagtggt ccgcacgcaa ccgcggcgcc ttctgcgagg gctacgccga
     2521 ggtggccggg cgcgacccgc gcgaggacga ggtgctgctg cgggcctacg agaccgacaa
     2581 ggccgtctac gaagccgtct acgaggcgca gaaccggccc gcctggctgg ccatccccct
     2641 ggcggccgtc gagcggctcg ccacctgatg acggccaccg agcgcccggt cgagcgcgcc
     2701 acgctcgagg cgctggtcgg cggggacctg gccgacccgc acgcggtgct cggggcgcac
     2761 ccgcacgagg gagcggtgac ggtgcgggtg ctgcgcccgt gggcctcgcg ggtggccgtc
     2821 acctacgccg accagcgggt cccgctccgc cacgagcacg acggggtgtg ggtcggcacg
     2881 ctgccggtgc cggaggtgcc cgactaccgg ctcgaggttg cctacggcgg cgagcccggc
     2941 ccggccgacg acccctaccg gttcctgccg acgctgggcg agctcgacct gcacctgctc
     3001 gccgagggcc gccacgagca gctctggtcg gtgctgggcg cacacgtgcg ccgctttgac
     3061 tcccccgccg gcccggtgac gggtacgtcg ttcgcggtct gggcgcccaa cgcgagaggc
     3121 gtgcgggtgg tcggcgactt caactactgg gacggccggg cccacccgat gcgctcgttg
     3181 ggggccagcg gggtgtggga gctgttcgtc cccggtgtcg aggccgggtc gacctacaag
     3241 ttcgcgatcc tcgaccgcga cgggcactgg cacaccaagg ccgaccccgt cgccttcgcc
     3301 accgaagcgc cccccgcgac cgcgtcggtc gtctacacgt cgacctacga gtggcacgac
     3361 cacgactggc tggcccgtcg tgccgcgcac gacccgcaga ccgggccgat gagcgtctac
     3421 gaggtgcacc tgggctcgtg gcggcccggg ctcggctacc gcgagctggc cggcgagctg
     3481 gtcgactacg tgcgcgagca ggggttcacc cacatcgagc tgctgccggt cgccgagcac
     3541 ccctacggcg gctcgtgggg ctatcaggtg acgtcctact tcgccccgac cgcccgttac
     3601 ggctcgcccg acgacttccg ctacctcgtc gaccagtgcc accaggccgg catcggggtg
     3661 atcctcgact gggtgccggc ccacttcccg cgcgactcct gggcgttggc ccgcttcgac
     3721 ggcacagcgc tctacgagca ccccgatccc cgccgcggcg agcagcgcga ctggggcacg
     3781 ctggtcttcg acttcggccg ccgcgaggtg cgcaacttcc tggtggccaa cgcgttgttc
     3841 tggctcgagg agtgccacat cgacgggctg cgggtcgacg cggtggcctc gatgctctac
     3901 ctcgactact cgcgcaacga gggcgagtgg gtgcccaacc gcttcggcgg ccgcgagaac
     3961 ctcgaagcgg tggcattcct gcaggagatg aacgcgaccg tctacaagcg ggtgcccggc
     4021 gtcatcacga tcgccgagga gtccaccgcc tggccgggcg tcacccggcc gacccacctg
     4081 ggcgggcttg gcttcgggtt gaagtggaac ctgggctgga tgcacgactc gctggcctat
     4141 ctgcgccatg agccggtgca ccggcagttc caccaccacg agatgacctt ctcgatgatg
     4201 tacgccttca ccgagcactt cgtgctgccg ctctcgcacg acgaggtggt ccacggcaag
     4261 ggctcgctgc tgcgcaagat gcccggcgac cgctggcagc agctggccac gctgcgcgcc
     4321 tacctcgcgt tcatgtgggc ccatcccggc aagcagctgg tgttcatggg tgccgagctc
     4381 gggcaggagg ccgagtggtc agaggcgcgc tcgctcgact ggtggctgct cgagcacccc
     4441 gaccacgccg gcgtgcagac cctcgtgcgc gacctcaacc gggtctaccg ccagaccctg
     4501 gccctgtggt cgctcgacca cgagcccgac gggttctcct ggatcgacgc caacgactcg
     4561 ggcaacaaca cgttctcgtt cctgcgccac ggcaccgacg gctcggtgct cgcgtgcatc
     4621 gccaacttcg ccgcggtccc gcacgagggc tatcgcctcg gcctgccggc ggccggccgc
     4681 tgggacgagc tgctcaacac cgacgccgag gtctacgccg gctcgggggt cggcaacctc
     4741 gggcgggtcg aggccgagcc ggtgccctgg cacggccagc cggcgtcggc gacgctgcgg
     4801 gtgccgccgc tgggcaccct gtggctgcgc tacgcgggtt cgagccggac cacgaccgcc
     4861 ttggagaccg gggtgttgct gcccttggcg gtcgagtcga gcggcacgag ctcgttggcc
     4921 tccgggaagt aggcggcggc gcagccgcgg gcggtcggga acgcgatgac ccggaacccg
     4981 cggacgaccc ggtcgacgcc atcggcccac tcgctgtgca cgtcgaccaa agtgccgtcg
     5041 cggacgccga gctcggccag gtcgtcgggg ttcacgaaga ccacccgccg ccccttgcgc
     5101 accccgcggt agcggtcgtt gagcccgtag atggtggtgt tgaactggtc gtgcgaacgc
     5161 acggtctgca gcaggagccg gccaggtggg cactcgacgt gctcgagctc gttgaccgcg
     5221 atcacggcct tgccgctggg ggtgggaaag gtgcgtgagt cgcgcggtcc gtggggcagc
     5281 acgaacccgc cctcgcggcg gaccttggcg ttgaagtcgt cgaagccggg gaccacccgg
     5341 gcgatgtgct cgcggatcag gtcgtagtcg cgttcgaagc cggcccagtc gacggccacc
     5401 cgctcgccca gcaccgcccg ggccagccga gtgatgatcg agatctccga gagcaggttg
     5461 ggcgaggccg gctcgaggac accctgcgag gcgtgcaccg cgcagaccga gtcctccacc
     5521 gagacgaact gcggcccggt ggcctgcagg tcgatctcgc tgcggcccag cgtgggcagg
     5581 atcagcgcct cccggccaac gacggcgtgc gagcggttga gcttggtcga gatctgcacg
     5641 gtcagctcgg tggcggccag cgcggcctcg gccaccgcgg tgtccgagat cgccgagacc
     5701 aggttgccgc cgagcgcgac ccacaccttg atccgcccgt cgcgcatccc ccggatcgcc
     5761 cggacgctgt ccacaccgtg ctcgcggggc ggctcgaagc cgaactcggc ggccaacgcg
     5821 tcgaggaacc gcttgggcat ctgctcccag atccccatcg tccggtcgcc ctgcacgttg
     5881 ctgtgcccgc ggatcggcgc cgccccggcg ccgggtcgtc cgatgttgcc ccgcagcagc
     5941 agcaggttga tgacctcgcg gatggtcggc accgccgccc ggtgctgggt caaccccatc
     6001 gcccaggtga tgatgacctt gtcggccttg atgtagcggg cggccagctc gtcgatctcg
     6061 gcggcctgca cgccggtcgc ccgagtgacc gcctcctcgt cgagggcccc gatgtgccgg
     6121 cggtaggcgt cgagcccctc gcagtgctcg gccaggaact cctggtcgag caccgtgccg
     6181 gggtgggcgg cctcggcctc gaagacccgc ttggccaccg cctgcagcag cgccatgtcg
     6241 ccgcccagcc ggatctggac gaactggtcg gccagcgggg tgccgcggcc gaggaggccg
     6301 ctgacccgct gagggttctt gaaccggatc agtccggtct cgggcagcgg gttgatcgcc
     6361 acgatctgcg ccccggccgc cttggcctcc tccagcgcgg tgagcatgcg cggatggttg
     6421 gtgcccgggt tctggcccat gatgatgatc aggtcggcct tggcgaagtc ggcgtagctg
     6481 atcgtgttct tgccgatccc gatgctctgg ctcatcgcca gcccggtgga ctcgtggcac
     6541 atgttggagc agtcgggcag gttgttggtg ccgaaggccc ggacgaacag ctggtagagg
     6601 aacgcggtct cgttggcggt gcgcccgctg gtgtagaacg cggcctcatc cggggactcg
     6661 agggcgcgca gcttgtcggc gacgatgccg atcgcctcag cccaggtgat gcgccgatag
     6721 tggtcgtcgt cggccggttt gtagaccggg tcgacgagcc gaccctgctg acccagccag
     6781 tactccgagc gccctcgcag gtcggtcacc gagtgctcgg cccagaagtc gggcgggacg
     6841 gtgagcgggg tggcctccca ggtgagcgcc ttcagcccgt tctcgcagaa ctcgaccttc
     6901 ttgcggtggt cggggtccgg ccacgcgcag ctcatgcagt cgaagccgtc cttctggttc
     6961 atcttcgacg cggagaccac cgcccggccg agccccatct cacggatcgc cgggaccacc
     7021 gagtggcgca ccccaggcag acccgccgcc gcagtggccg ggcgccgcac gcccaggtgc
     7081 tcgtcggtga cgtcctccac gagcggcttc ctcgtcacgc tgcgctccct gcggccggct
     7141 ggcacccgat gtagccccta gcatcgtcgg gtcgcccgac ccgacgcaac gatcagctcg
     7201 cctgaccgag cgtggccacg acggcccggg cgaaccggtc ggccgcgccc gggtcatggc
     7261 cgaggaacat cgacggctcc gtgacctcca gctccatcag ccgtggcgtg ccgtcgtcac
     7321 ccggcacgag gtcgacccgg gcataggtca acgacgcgcg ggggcagggc agccggtcga
     7381 gcacgtcctc ggccgccgcc cgctcggccg cgctgggcac ccgggcctcg atgtactcct
     7441 tgccgggcgg gccgccagcg gtgccgagca tcggcgcctt gcggatggcg tggctgaagc
     7501 ggccggcgaa gaacagctgc gcggtctcac cggcggtgtc gaccgcctcc agatagggct
     7561 gcaccatcac ggtgcgtccg gccgccagca gccgcccgac gtgctcgtcg gccagccggt
     7621 cgtgctcgcc gctgcgataa cgggcggtgt cgagcgagcc ggccgacacc gcgggcttga
     7681 cgacgtagtc gccgccggcc ggcgcgacga acgtgtcgcc cgggtgcagc caagtcgtgg
     7741 gcacgaccgg ggcaccggcg gccgcgaggt cgcgcagata ggtcttgtcg gtgttccagc
     7801 gcagcacctc aggcgggttg gccagccggg tgaccccggc cacccgctcg atccaggcca
     7861 ggaactcgtc gcggcggtgg aagtagtccc aggtcgagcg caccagcacg aggtcgtagg
     7921 acgaccagcg cgcgtgcggg tcggtccaca cgaccggctc ggccgccacc tcgtgcctgg
     7981 ccagcgcgtc gagcagcagc gggccgtcgg cggccaggtc ggggtaggcg gccgcggtgg
     8041 ccagggcgat acggggagtc accgtggcag cctagaaccg gcgtccaagc aggtcagcgg
     8101 ccgatcgcgt gcccgaagcg gaacatcccg gccgggtcga gctcgcgcac cagggcgcac
     8161 aaccgttggt aggtcggctc gtcccaggcg cgggcccggt cggcctcgtc gcccggcacg
     8221 ccgtggaagt tgaccatcgt gcgcccggtc gaccaggggg caagtgctgc gacggtcgac
     8281 tccaccgcgg cgagcgcggc ctcgggcgcc ggacccatcg ggatcgccac cgtctccacg
     8341 ccgaaggccg cgtcacggcc gccgaccgcg ttgggcaccg cggcaggacg ggccagggcc
     8401 ccgcccagga gccggatctc gcacatcagc aggggcgtct cgacctgcgg cccggcgacg
     8461 ccgaggaccg cctcgacggt gtcctcggtc agctcacgca gcagggtgcc ctgctccacg
     8521 aagggcaccg ggtggtcggg gtcctggtgg atgctgtcgg cctcgacaaa gcgcatcggc
     8581 ccgacggcgt ccatcatggc cggcgcagcg gcccgcatcg gcgcgagcag cgcctcaccc
     8641 tcggcgacgt caccgaggtg gcagaagcgc aggtgagcca cggtctgccc gcgcagcggc
     8701 tcggggacct ccgggaacgg cggcacgcgc atcaccgcga tcgaggccga ggtgtgctcg
     8761 ggcaggtcgg gcacccaggt ccggaacgcg tgcaggagcg tcgggatgtg ctcggcggcg
     8821 tagaagatgc caccgccgaa gaccgtgtcg acctcgacga gctcgaccgt catctcagtg
     8881 acgatgccca ggttgccctt gccgccacgc agcgcccaga acaggtcggg ctcgctcacc
     8941 gcgtcgacct ggcgcacccg cccgtcggcg gtgaccagct cgagcgagac cacgtggtcg
     9001 gcgctgaagc cgaacgtccg gcccaggatc ggcagcccgc cgcccagggt gtagcccacg
     9061 acgctgacgt ctgacgtcga gcccgacagc ggcgccagcc cgtgcggggc cgcggcatcg
     9121 agcaccgccc gccagcgcac gcccgccccc acggtcgccg tacggcgtgc ggggtcgatc
     9181 gtcaccgagt cgagggcccg ggtgctgacg agcagccccc cgtcgatcgc cacctcggcg
     9241 ccgtggccgg tggcctgcac cgcgaccggc agcgcgtgcg cgccggcgta gcgcaccgcg
     9301 gcggccacgt cctgcgcatc cgcagcgacc acgacgagcc gaggggtgtg cgcgacggcc
     9361 aggttgtagg ccgcgacctc ctccggccag cccggcgagt cggggcgcag gacgctcccc
     9421 cgcacggtgg ccgcaagctc gtcgaacgac tcgtcgatgg tgcgggtgct ggccatgggt
     9481 ccccctggac acgttgtcgg gcctgcccgc cacgatgggc ggcgcacggc cgaccggcac
     9541 cctagccgat caatggcgcg ggcggggacc tccggcccta ggcgcagcgc aaccgcgctg
     9601 cgacgcttcg acgaccgtcg gggggagcct gaatggtcac gaccggagct cgcccgatcg
     9661 ggccggtcga cgcgatctgg ctcaacatgg accggcccaa caacctcatg gtgatcgacg
     9721 ccctgctgtg gctgcacgag ccgatcacac gtgcacgcct gggcgaggtc gtccgacaac
     9781 ggctcgtcga gcgctacccc gtcttccggc aacgcccggt ccccggcggg gcgccatcgg
     9841 cgagcggatg gacgcgcagg cattgggagg aggaccccga gttcgcactg gaccggcacg
     9901 tgcgccgagc ccggctgcgt cccccggggg acgacgcggc gctgcatcac ttcgtcgacg
     9961 cccgcctgag ccggcccttc gaccgccgcc acccgctctg ggagatccac ctcatcgacg
    10021 gctacggctc cggcgccgtg gtcctggccc ggatgcacca cgccctggcc gacggggtcg
    10081 ccctggcgca ggtgctgctc tcgctcaccg acgcgtcggt gggcagcgat ggtggcgttg
    10141 ccgatccgcc gcgccccgcc gcccctgcaa gggggcccgc cgacacggtc gctgccggca
    10201 tctcgctggt acgccgctac gcgaccccgc gtgggctcgt cgaggtgctg gccttggccc
    10261 gccaggcggg gggcgtggtc ggcaagctgg tgctgggctc caacccggcc acgccgttga
    10321 gcggacgccc gggcgtcgcc aagcacgcgg tctggtcagc gccgcggccg ctgcagcccg
    10381 tcaagacgct cgggaagatg accggcgcca cggtcaacga cgtgctggtg tgcgcggtgg
    10441 ccggtggcct ctcccgctac ctcgccgagc acggcagccc accggtggac ctcacgacga
    10501 tggtgccggt caacctgcgt ccccagggcg tgccactgcc tgcggagctg ggcaaccgct
    10561 tcgcgctcgt gctgctggag ctgcccagcg gcaggtggcc ggtgctggcc cgtctggccg
    10621 agaccaaacg ccgcatggat gccatcaagc actcccccga ggcggccatc acctttggcc
    10681 tgatcagcgc gatcggccgg gccaccccct acgtcgacca gcccctcgtc gacttcttct
    10741 cgtccaaggc gttcggggtg accaccaacg tcgccggccc gacgaccgag cggtcactgg
    10801 ccggcagcca ggtggacagc gtgctcggct gggtgcccgg ctccggcgac cagggagtcg
    10861 gagtctcgat cgttacctac aacggcaccg tccggctggg cttcaaggtc gatgctgtga
    10921 tcgtgccgga cccagaccgg ctggtggcag ccgccgaggc gtctctcgac gagctgctgc
    10981 ggatcacccg gaacgcctga ggtcaaggag gcatggatgt ccaccgacgt cgccacggac
    11041 accatcgagg tcgacgcccc gcccgcgcag gtgctcgcca ccctgcgcga cgtcgcggcg
    11101 caaccgcact gggtgcgtga ggtcacctcg gtcgacctgc tcgacaccga cgagcagggg
    11161 cgcccggcca ccgcccgggt cacggccacg acgccgatgg gcaccgacga gtacaccctc
    11221 gcctatgagc acaccgacga gggcatgagc tggtcgatgg tcgagggccg cctgcagacc
    11281 ggccaggacg ggcactacac gctggagggg ctcgacgacg accggaccag ggtcacgttc
    11341 gagctggcga tcagccacaa cctgccgctg cccggcttcc tcacccggca ggtggtcaac
    11401 ggcgtggtca cgggcacgct ggccgggctc aagcgctacg tcgaggagcc ggccgggtcg
    11461 acccactgac cggcggccgg caggcctaac cggctggaca ggctcagaac agggcgttgg
    11521 ccagggccag gcgggccggg ccgaccctgg ggtcctcggg ccccaccacg tcgaagagtg
    11581 cgatcaggtg ctcgcgtgcc gtgttgcgat cctcgcctga ggtggcgcgc accgtgtcga
    11641 tgaggcgcgc gaacgcgtcc tcgacgtggc ccccgagcag gtcgaggtcg gccgccacga
    11701 tctgagcggc cacgtcggcc gggttctcgg ccgccgcagt gcgggccgcc gccgggtcga
    11761 cgtcttgggt gcgggccagc agctcgaccc gagccagccc caaccgggcc acctcgtcgg
    11821 ccgggcgggt ggccagcacc gcccggtagg ccgtcgccgc gccgtcgtag tcgcccgcga
    11881 gcatcgcgtc ctcggcggcc tgcaggtcgg gtggcagcgc cggctcggcc ggctcgacgg
    11941 cggtctcccc gtcggcgggc tcgccacgcg taagccggcc ggagacaccg ttggcctcgg
    12001 cgacccgcag cagctcgtca agcacctggc ggacctgcgg ctcgggcacg gctccctgga
    12061 acagcgggac catctgcccg ccgaccacgg cgaccagcgt cgggatgctc tgcacgccga
    12121 acgccgcccc gagccgctgc tcggcgtcga cgtcgacctt ggccagcagc cagcgtccgt
    12181 cgtactcctc gaccagcttc tcgagcaccg ggctgagctg cttgcacggc ccgcaccact
    12241 cagcccagaa gtcgatgacg accggcacgg tctgcgagcg ctcgatgatc tcggcctgga
    12301 acgtcgcctc ggtcacgtcg acgaccagcg acgagacggc gttggccccg gaggcgctcg
    12361 ccgcctcggc cgccgcgcgc cgttcgttgg ccgcggccac ggcggccagg tcgacggcgc
    12421 ctcggacgtt gatggcggga ccggcgctgc cacgaggtgt catcggcgta ctcatgactc
    12481 ctagtctccc tgatgactcg actcgcactg acctgacccg cccccgggct cagaaacggg
    12541 ccggttcgcg gtagtcgccc cagacctggc gcagtgcccc gcagatctcg cccagggtgg
    12601 cctcggcccg ggcggcctcg agcatcggcg gcaccgtgtt gccctcaccg cgggcgacct
    12661 cgaccattcg ggccagcgcg gcgtcgacac cggcctggtc acgggcggtg cggcgctgac
    12721 ccagcgcgcg gttctgctcg acctcgacct cgtgggagac ccgcaggatc tccaacggct
    12781 cctcgaccgt cgaggtgtgc gagttgacgc cgacgacctt cttctcgccc ttctccaggg
    12841 cctgctgata gcggaatgcc gcctcggcga tctcggccat gaaccagccg tcctcgatgc
    12901 cgcgcagcag ccccgaggtg atcgtgccgt cgcctcccat gtcgcggatc cgggcgaaga
    12961 tcgcctcggc ctcggcctcg agccggtcgg tcagctcctc gacgtaccac gacccgccga
    13021 gcgggtcggc cacgttgacg acgccggtct cctccatgag cacctgctgg gtgcgcagcg
    13081 cgatctcggc ggccttctcg ctgggcagcg caagcacctc gtcgagggcg ttggtgtgca
    13141 acgagttggt gccgccgaga acggcggcca gcgcctcgac cgccgtacgc acgatgttgt
    13201 tgttgggctg ctgcgcggtc agcgagacgc ccgcggtctg gctgtggaag cgcatgcgcc
    13261 acgacgcggg gtcgcgcgcg ccgtaggtgt cgcgcatctc gcgcgcccag atgcgccgcg
    13321 ccgcgcggta cttcgcgatc tcctcgaaga agtcgatgtg cgcgttgaag aagaacgaca
    13381 ggcgcggcgc gaagtcgtcg acggcgaggc cgcgctcgac ggcggcctgc acgtaggcga
    13441 agccgtccgc gagcgtgaac gcgagctcct gctgcgcggt cgagccggcc tcgcggatgt
    13501 ggtagccgga gatcgagatc gggtgcatcc gcggcagctc ccgcgcgcag tactcgacca
    13561 tgtccacgca cagccgcatc gacggctccg gcgggtagat gtactccttc tgcgcgatgt
    13621 actccttgag gatgtccgtc tggatcgtcc cccgcagccg gtcccgtggg acgccctgct
    13681 cctccccgac gaccaggtac atcgccagtg cga
//
LOCUS       DATIBB010000070         9187 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_691983, whole genome shotgun sequence.
ACCESSION   DATIBB010000070 DATIBB010000000
VERSION     DATIBB010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 9187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9187
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_691983"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(169..2013)
                     /locus_tag="VLC50_02465"
     CDS             complement(169..2013)
                     /locus_tag="VLC50_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010146287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF294 nucleotidyltransferase-like
                     domain-containing protein"
                     /protein_id="HSF26360.1"
                     /translation="MDVELSEVRDFLATHAPFDVLPSGVLDLVPRQCTLRYARRGTVV
                     LEVGEQGAGLYVVRSGAVDVVDEAGGLVERVGTGVAFGMSSLLEQRPTRYRCTATEDT
                     LLLVVPAELFETLSREHTGFATFYAATHHARLRKAIGNLQQAASGSTVLGTLVRDLVT
                     HEPVTIAPEATIEQAAAAMSQGGVSSILVVEDGCLAGIVTDRDLRQRVLAVGLDPRRP
                     VREVMTSPALTLRADAAAFEALLEMVSRRIHHLPVVDDRGAAVGLVTSTDLIRLENSN
                     PVYLAADIGRQTSVAGVVEQAHRIPTVLGQLVDRDVSAADIGRVVTALGDAVRRRVLG
                     LVEAELGPPPAPYAWVVLGSVAREEEALSADQDHALVLAEPGHDDWYAGLANRVTEIL
                     VECGWPRCPGDVMATNPRWRLTVDQWRRHFAAWSAEPDPDSTLQVAIFHDMRHLAGDP
                     RLTTEVRREAASFVSTRLLGHLGAQALRMGPPLGFFRGFVLEREGDNRDTLDIKRGIA
                     AVVQLARVHALRAGSTALSTRARIAAAAEAGVIDEEAAADLADALELMSYRRLHHQVA
                     QVRAGERPDNRIAPADLTERQRRHLKDAFAIVRSAQQRLANRLEPGYV"
     gene            complement(2065..2433)
                     /locus_tag="VLC50_02470"
     CDS             complement(2065..2433)
                     /locus_tag="VLC50_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007633817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HSF26361.1"
                     /translation="MPTKPETVDDYIASFPAPAQQRLRELRELSRATAPHAVEGLKWG
                     NPAYSVETILFIFSGHKQHANFVVTPTTLSAFMDEVAGYQTGKGSVQLPYDEPAPTDV
                     LAKMIAYRVKEWEERGVRWM"
     gene            complement(2500..2709)
                     /locus_tag="VLC50_02475"
     CDS             complement(2500..2709)
                     /locus_tag="VLC50_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003881797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HSF26362.1"
                     /translation="MADIEDLGPEFDGVRIGRPAVGALLRAGYRTLSDLPEELEGLLE
                     LHGVGPLAVRLLRQAREDREHSRDK"
     gene            complement(2760..3293)
                     /gene="cpt"
                     /locus_tag="VLC50_02480"
     CDS             complement(2760..3293)
                     /gene="cpt"
                     /locus_tag="VLC50_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015655637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloramphenicol phosphotransferase CPT"
                     /protein_id="HSF26363.1"
                     /translation="MPTQVIVLNGGSSSGKSGIVRCLQVLLPDPWLALGVDSLVDALP
                     AKMQASDAGITFGADGEVSVGADFVALESAWMEGVAAMACAGARVIIDDVFLGGAASQ
                     QRWQKFLGGLAVLWVGVRCESAVAAGREVARGDRAQGMAALQADVVHEGVVYDLEVDT
                     THTESLVCARTIAAHVS"
     gene            complement(3384..4610)
                     /locus_tag="VLC50_02485"
     CDS             complement(3384..4610)
                     /locus_tag="VLC50_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010072861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsO family NAD(P)H-dependent flavin-containing
                     monooxygenase"
                     /protein_id="HSF26364.1"
                     /translation="MSSTLPTEVDVAIIGGGQSGLAVGYQLRRHIRRPGNQRPSFVIL
                     DDHPQPGGAWQDMWGTLHLFSPAGFSSLPGWPMPPWSGEDNPDTAHVVKYLAAYEERY
                     ELPVERPVTVTAVRRTADEGRLLVETDEARTWSARWVVNTTGTWRRPFWPNVPGMADF
                     AGRQLHTVSYSGAAAFAGRRVLVVGGGNSGAQIAADLLPVAASVTWATKHPPRMLPDD
                     VDGRILFETATRAVEDRVAGIANDGVASLGDIVAVPSVRHARDHHGLHAEPMFEHLVA
                     GGAVWADGKERPYDAVIWCTGFRPALRHLAPLALSTRQGRPRTTGFAQLDVGSTRHRS
                     PVVSVNDPRVLFVGYGDWCGPASATLIGVNRAARDAAATVLAERVASEPAGGSIVTDP
                     PALKVGWSGPRAGLRG"
     gene            complement(4607..5902)
                     /locus_tag="VLC50_02490"
     CDS             complement(4607..5902)
                     /locus_tag="VLC50_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017987834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSF26365.1"
                     /translation="MNPTSGSAEPAVTLDRGLPRAALRRVTAVLCATEIVSWGILYYA
                     FPVLAPSISDDTGWSMTAVVAAFTVAQLVAGGVSIVVGRRIHEHGPRGVMTLGSVVAV
                     ASVLLLAQAGSWWWFLAAWCVAGAAMAATLYAPAFAALTIWGGPGRLQALTALTLVAG
                     LASTVFAPLAAVLDDRLGWRDTYTVLAALLAVTVVLHSWGLRAPWPTTDPPLGAAPGP
                     ADPVGLGATVTMSGRVSPWRVPGFTRTVAAFTLAGVCIWSVIIVFVPLLTARGFSTAA
                     AATALGIGGIGQVCGRLGYRRLDAATSTATRTRLVFAGVAITTLALAITPGPYVALVI
                     LSFLAGMARGVYTLLQATAVTDRWGASDYARLNAALTLPLMASAAFAPWFATALAAGL
                     GSHAAALVVCAALAAASLAVVPARGARAAAPMVTDAPVR"
     gene            complement(5915..7303)
                     /locus_tag="VLC50_02495"
     CDS             complement(5915..7303)
                     /locus_tag="VLC50_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015659444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HSF26366.1"
                     /translation="MSTAAPSSLVAGVTPSADLPLVIVGAGPIGLAAAAYAQSRGLAT
                     IVLEAGPTAGASVREWGHVRLFSAWRELVDPAAAKLLAPTGWVAPDSDSYPTGTDWVE
                     QYLAPLAGALADTDEVEIRYGHRVVGLARHGRDRLVAADREGLPFTVHVETSDGTRVR
                     LNAAAVVDASGTWTGPNPLGGDGYPAAGEAEHANRIAYDIPDFTDLDVADRYAGKHVA
                     VAGAGASAQNVLVGLSELAKAHPGTRVTWLVRRPGTDDAFGGGDNDQLEQRGALGERA
                     KRAVADGPVTVVTSFRTSDVERSEDGRLRLTSFADLGTGASPSAAGKVVDGVDEVIVV
                     TGFRPDLSWLTQVQLDLDGELDAPAQLAGEIHPAWHSCGNVTPHGADRLRQPESGLYL
                     AGMKSYGRAPSFLAMTGFEQVRSVVAEIAGDHEAAARVELVLPETGVCGGSGRFDGEP
                     TAERGGCCGTCG"
     gene            complement(7765..8643)
                     /locus_tag="VLC50_02500"
     CDS             complement(7765..8643)
                     /locus_tag="VLC50_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010595533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-70 factor"
                     /protein_id="HSF26367.1"
                     /translation="MSEDPFLAHRSLLFTVAYEMLGSASDAEDVVQETWLRWASLDET
                     ERAEVHKARAYLVRIVTRLSLNRLRTLARQREDYVGQWLPEPLLTSPDVAEDVELAES
                     VSIAVLAVLETLLPTERAVFVLREVFDVPYDEIAEALDKSPAAVRQIAVRARKHVAAR
                     RPRMSVSRTEQDQVVERFLAALTTGDVPGLLKVLAPDVLVVGDGGGLAPTIPKPVRGA
                     AKLVPVMASFAKLAPGAKAFIVELNGGIAARIDPGGENDTAVSFVIENGRITQIFAIR
                     NPHKLGRLGEVAQLRR"
     gene            complement(8640..>9187)
                     /locus_tag="VLC50_02505"
     CDS             complement(8640..>9187)
                     /locus_tag="VLC50_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HSF26368.1"
                     /translation="KGALVKRMILKRLGKVPTSVSVYWHNPRVLFASFGFGGKLEKWD
                     ACDESLKSFAHMAVASLVGCTWCLDFNYFEVRNRGLDVDKAREIPRWRESDVFTPLER
                     DVLEYAEAMSQTPPTVTGELVDRLNAQLGPAAVVELTSVIGFANLTTRGNVALGIESD
                     GFAAACGMKPLAERPGVASAS"
BASE COUNT         1401 a         3215 c         3324 g         1247 t
ORIGIN      
        1 cgtggcgcgg gtcgaagccg gtcgtctcga ggtccacggc gaggaagtcg acctgcgaca
       61 ccgtggtgcg ggacggcggc ggcggcgcgt cggccaggtc gcggaccgga ccggccgggt
      121 agcgccgcgg gtcgggcggc cggtcccggc gcgcgaacca gcggcccatc agacgtatcc
      181 gggctcgagc cggttggcca gccgctgctg cgccgagcgc acgatcgcga aggcgtcctt
      241 gaggtggcgg cgctggcgct cggtcaggtc ggctggggcg atgcggttgt cggggcgctc
      301 accggcacgc acctgggcca cctggtggtg caaccggcgg taggacatca gctccaacgc
      361 atcggcgaga tcggccgcgg cctcctcgtc gatgaccccg gcctctgccg cggccgcgat
      421 ccgggcccgg gtcgacaggg cggtcgagcc cgctcgcagc gcgtgcaccc gggccagctg
      481 caccaccgcg gcgatgccgc gcttgatgtc gagggtgtcg cggttgtcgc cctcgcgttc
      541 gagcacgaag ccgcggaaga agccgagcgg ggggcccatg cgcagcgcct gcgcgccgag
      601 gtggccgagc agccgggtcg agacgaacga ggccgcctcc cggcgtacct ccgttgtcag
      661 ccggggatct ccggccaggt ggcgcatgtc gtggaagatc gcgacctgca gcgtcgagtc
      721 ggggtccggt tcggccgacc acgcggcgaa gtgtcgacgc cactggtcaa cggtgagccg
      781 ccagcggggg ttggtggcca tcacgtcgcc ggggcagcgt ggccacccgc actcgaccag
      841 gatctcggtg acccggttgg ccagcccggc gtaccagtcg tcgtggccgg gttcggcgag
      901 cacgagcgcg tggtcctggt cggccgagag cgcctcctcc tcgcgggcga ccgacccgag
      961 caccacccag gcgtacggcg ccggcggcgg acccagctcc gcctcgacca gccccagcac
     1021 ccgtcggcgc acggcgtcgc ccaacgccgt caccacccgg ccgatgtcgg cggcggagac
     1081 atcccggtcg accagctgac ccagcaccgt ggggatccgg tgcgcctgct cgaccacccc
     1141 ggccacgctg gtctgccggc cgatgtcggc ggccaggtag accgggttgg agttctccag
     1201 ccggatcagg tcggtgctcg tcaccaggcc gacggccgcg ccccggtcgt cgaccaccgg
     1261 caggtggtgg atgcgccggc tgaccatctc gagcagcgcc tcgaaggccg ccgcgtccgc
     1321 gcgcagggtc agcgccggcg aggtcatcac ctcgcgcacc ggccggcgcg ggtcgaggcc
     1381 caccgccagc acccgctggc gcaggtcgcg gtcggtcacg atcccggcga ggcagccgtc
     1441 ctccacgacg agaatgctcg agacgccccc ctgggacatg gccgccgcgg cctgctcgat
     1501 cgtggcctcc ggcgcgatgg tcaccggctc gtgggtcacc aggtcgcgca ccagcgtgcc
     1561 cagcacggtg ctgcccgacg cggcctgctg caggttgccg atcgccttgc gcagccgggc
     1621 gtggtgcgtg gcggcgtaga aagtggcgaa gccggtgtgc tcgcgggaca gcgtctcgaa
     1681 gagctcggct ggcacgacca ggagcagggt gtcctcggtg gccgtgcacc ggtagcgcgt
     1741 gggcctctgc tccagcagcg agctcatccc gaacgcgacg ccggtgccga cccgctcgac
     1801 cagtccgccg gcctcatcga ccacgtcgac ggccccggag cgtacgacgt acaggcccgc
     1861 gccctgctcg cccacctcga ggaccaccgt gccgcggcgg gcatagcgca gcgtgcactg
     1921 ccgcggcacc aggtcgagca cccccgacgg cagcacgtcg aagggcgcgt gcgtcgccag
     1981 gaagtcgcgc acctccgaca gctccacatc catgccacgt atggtggcat ttgccgccct
     2041 gccgtgtcgt cggctcgagg ccacctacat ccagcggacg ccgcgctcct cccactcctt
     2101 gacccggtag gcgatcatct tggcgagcac gtcggtgggc gcgggttcgt cgtacggcag
     2161 ctgcacggat cccttgccgg tctggtagcc cgcgacctcg tccatgaagg cgctcagcgt
     2221 ggtcggggtg acgacgaagt tggcgtgctg cttgtgcccg ctgaagatga acagaatcgt
     2281 ctccaccgag taggcggggt tgccccactt gagcccctcg acggcgtgcg gcgcggtggc
     2341 ccgactgagc tctcgcagct cacgcagccg ttgctgcgct ggcgcgggga acgacgcgat
     2401 gtagtcatcg acggtctcgg gcttggtcgg catgtggtga gcctgtcacg tgcggagtgg
     2461 gaatcgttgg cgtgcaacga ctcgatcact cctgcacgac tacttgtctc gcgagtgctc
     2521 tcggtcttcg cgcgcttgcc ggagcaggcg caccgccagt ggcccgacac catggagctc
     2581 gagcaggcct tcgagctctt cgggcaggtc gctcagggtc cgatacccgg cacgcagcag
     2641 cgcaccgacc gcggggcgac cgatccggac tccgtcgaac tccggaccaa ggtcctcgat
     2701 gtcagccatg gtcacctcag acgttgaacc gaaacgttca gccgggagtc gtacgtcgat
     2761 cagctgacgt gggcggcgat ggtccgcgca cacaccaggg actcggtatg cgtcgtgtcc
     2821 acctccaggt catagaccac accctcgtgg accacatccg cctgcaacgc cgccatcccc
     2881 tgggcccgat ctccccgtgc gacctcacgg cctgcagcga ccgcgctctc acacctgacg
     2941 ccgacccaca acacggccag tccacccaag aacttctgcc accgctgctg ggacgccgct
     3001 ccgccaagga agacgtcatc gatgatgacg cgggcaccgg cacatgccat cgcggccacg
     3061 ccttccatcc aggccgactc cagcgccacg aagtccgccc cgacgctcac ctcgccgtct
     3121 gcgccgaacg tgatccccgc atccgacgcc tgcatcttgg cgggcagcgc gtcgaccaac
     3181 gagtcaaccc ccaacgccag ccacggatcc ggcagtagga cctgcaggca ccggacgatc
     3241 ccggacttcc ccgagctgga accaccgttg agaacgatca cttgagtcgg caccgcgcca
     3301 ccgtaggacg cttcccacac agcacgaaac gcaattggat agacgctgat cggctgcctg
     3361 cacgacacgt tgaagcgaaa cgatcagccc cgcaggccag cacgaggccc ggaccagccg
     3421 accttcagcg cgggcggatc ggtcacgatc gatccgcccg cgggctcgga tgcaacccgc
     3481 tcggcgagca cggtcgcggc tgcatcgcgg gcggcccggt tgacgccgat gagggtggcg
     3541 gaagcgggcc cgcaccagtc gccgtagccg acgaagagga ctcgcgggtc gttgaccgac
     3601 acaaccggcg accggtgccg cgtcgagccg acgtcgagct gcgcgaatcc ggtggtgcga
     3661 ggacggccct gcctcgtcga cagcgcaagt ggcgcgagat gcctgagcgc gggccggaag
     3721 ccggtgcacc agatgaccgc gtcgtacggg cgctccttcc cgtcggccca cacggcgccg
     3781 ccggcgacga ggtgctcgaa catcggctcc gcatggaggc cgtggtggtc gcgagcgtgg
     3841 cggacggagg gcacggccac gatgtcgccg agcgacgcga ctccgtcgtt ggcgatgccg
     3901 gcgacgcgat cctcgacggc gcgggtcgcg gtctcgaaca ggatgcggcc gtcgacgtcg
     3961 tcggggagca tgcgcggcgg atgtttggtc gcccaggtga ccgacgcggc aacggggagc
     4021 aggtcggcgg cgatctgggc gccggagttg ccgcccccga cgacgagaac gcgacggcct
     4081 gcgaacgcgg cggcgccgct gtagctgacg gtgtggagct gtcggccggc gaagtcggcc
     4141 atcccaggga cgttcggcca gaagggtcgg cgccaggtac cggtggtgtt gacgacccac
     4201 ctggctgacc aggtgcgtgc ttcgtccgtc tcgacgagga gtcggccctc gtccgccgtt
     4261 cgacgaaccg cggtgacggt gacgggacgt tcgacaggca gctcgtagcg ttcctcgtag
     4321 gccgcgaggt acttcacgac gtgggcggtg tcggggttgt cctcgccgct ccacggcggc
     4381 atgggccagc ccggcagtga gctgaagccg gctggcgaga acaggtggag ggtgccccac
     4441 atgtcctgcc aggcgccgcc gggctgcggg tggtcgtcga ggatgacgaa cgacgggcgc
     4501 tggttgcccg ggcgccgaat gtggcggcgc agctggtagc cgacggccag gccgctctgc
     4561 ccgccgccga tgatggcgac gtccacctcg gtgggcaggg tggagctcat cggaccggcg
     4621 cgtcggtcac catcggcgca gctgccctgg cgccgcgggc ggggacgacg gcgagggacg
     4681 ccgcggcgag agcggcgcag acgacgagag cggcggcgtg gctgccgaga ccggcggcga
     4741 gggcggtggc gaaccagggt gcgaacgccg ccgaggccat cagcggcagc gtcagggccg
     4801 cgttgaggcg ggcgtagtcg ctggcgcccc atcggtcggt gaccgcggtt gcctgcagga
     4861 gcgtgtagac acctcgagcc atgccggcca ggaacgacag gatgacgagg gcgacgtacg
     4921 ggcctggggt gatggcgaga gcgagcgtgg tgatggcgac gccggcgaac acgagccggg
     4981 tgcgggtcgc ggtgctggtg gcggcgtcga gccggcggta tccgaggcgt ccgcagacct
     5041 ggccgattcc gccgatgccg agggcggtcg cggccgccgc ggtgctgaat ccgcgggcgg
     5101 tcagcagcgg gacgaacacg atgatgaccg accagatgca cactccagcc agagtgaacg
     5161 cggcgacggt tcgcgtgaac ccgggcaccc gccacggcga gacgcggcca ctcatggtca
     5221 ccgtcgcacc gaggccgacc ggatctgcgg gcccaggggc ggcccccaac ggcggatcgg
     5281 tggtgggcca cggcgctcgg agcccccagg agtgcaggac gacggtgacc gccaggagcg
     5341 ctgcgaggac ggtgtaggta tcacgccagc cgaggcggtc gtcaaggacc gcggccagcg
     5401 gcgcgaacac agtgctcgcg agaccggcga ccaaggtgag cgcggtgagg gcctgcaggc
     5461 ggcccggacc gccccagatg gtcagggccg cgaacgccgg cgcatagaga gtggcggcca
     5521 tggcggcgcc ggccacgcac caggcggcaa ggaaccacca ccacgacccg gcctgcgcga
     5581 gcagcagcac tgaggcgact gcgacgaccg acccgagggt catcacgcca cggggcccgt
     5641 gctcatggat acgccgaccg acaacgatgc tgacgccgcc ggcgaccagc tgcgccaccg
     5701 tgaatgcggc gacgacggcc gtcatcgacc agccggtgtc gtcgctgatg gacggcgcga
     5761 gcaccgggaa ggcgtagtag aggatgcccc aggagacgat ctcggtcgca cagaggacgg
     5821 cggtgacccg ccgcagcgca gcacgcggga gcccgcggtc gagagtgacc gcgggctccg
     5881 cgctgccgct cgtcgggttc atcaagcggg caggctagcc gcaggtgccg cagcagccgc
     5941 cgcgctcggc ggtgggctcg ccgtcgaacc ggccggagcc gccacagacg ccggtctcgg
     6001 ggaggacgag ctcgacccgg gccgcggcct cgtggtcgcc ggcgatctcg gcgacgacgc
     6061 tgcggacctg ctcgaatccg gtcatcgcca ggaacgacgg cgcgcggcca taggacttca
     6121 tcccggcgag gtacaggccg gactcgggct gccggagtcg gtccgcgccg tgcggggtga
     6181 cgtttccgca ggagtgccag gccgggtgga tctcgccggc caactgcgcg ggcgcgtcga
     6241 gctcgccgtc caggtcgagc tgcacctggg tgagccatga caggtcggga cggaacccgg
     6301 tgacgacgat gacctcgtcg acgccgtcga cgaccttccc tgccgcgctc gggctggcgc
     6361 ccgtgccgag gtcggcgaac gaggtcaatc gcagccgacc gtcctcggag cgctcgacgt
     6421 cgctggtgcg gaaggacgtg accacggtga cgggtccgtc ggcgacggcg cgcttggcgc
     6481 gctccccgag ggcaccgcgc tgctcgagct gatcgttgtc gccaccgccg aacgcgtcgt
     6541 cagtgccggg acgacgcacc agccacgtga cccgcgtgcc ggggtgggcc ttcgccagct
     6601 cgctgaggcc gaccaggacg ttctgcgccg aagcgccggc ccccgctacg gcgacgtgct
     6661 tgccggcgta ccggtcggcg acgtcgagat cggtgaagtc ggggatgtcg taggcgatgc
     6721 ggttggcgtg ctcggcctcc ccggcggccg gatagccgtc gccgccgagc gggttcgggc
     6781 cggtccaggt gccggatgcg tcgacgacag cggcggcgtt cagccggacg cgggtgccgt
     6841 cgctggtctc gacgtggacg gtgaacggga ggccctcccg gtcggccgcg acgaggcggt
     6901 cacggccgtg gcgagcgagg ccgacgaccc ggtggccgta ccggatctcg acctcgtcgg
     6961 tgtcagcgag cgcgccggcg agcggggcga ggtactgctc gacccagtcc gtgccggtcg
     7021 ggtaggagtc ggagtccggg gcgacccagc cggtcggggc gaggagcttc gcggcggcgg
     7081 ggtcgacgag ctcgcgccag gccgagaaca gccggacgtg gccccactcg cgcaccgatg
     7141 cgcccgccgt cggccccgct tcgagcacga tcgtggcgag gccacgggac tgggcgtacg
     7201 cggcggcggc taacccgatg gggcccgcgc cgacgatgac cagcggcagg tcggcgctcg
     7261 gggtcacccc ggcgacgagg ctggacgggg cggcggtgct catggcggct cctgacgatc
     7321 gcgagcatat cgacggctgt ctatggactg aatgtatcga ccgatatcga tcttggcaac
     7381 catcgatctg cgtcgatggt gaggtgggct gagtccatga ccgtcgagac cgtcgagccg
     7441 cgacaattac acccagggca tcgccacccg caacgccacc tgacggcaat cagcggccta
     7501 cccgctgtcg gggctggcgg tcaccgccgc cgcggccaac ctagacactg tgcggcgcgt
     7561 ggtcgcttct tcttcgcgcc atgcacacgt tctgcgcgtc gagccgacct caatcgcggg
     7621 cttgaaggtg cggctaggca gccgccaacc ttcatctgac ctgcggaaac gccccagctt
     7681 actgacaggc cctcgatccc agcaccgatg atgatgaccc gcatagtgac tccaagcggc
     7741 tccattggac ggcggcacac ggcctcaccg ccgaagttgg gccacctcgc ccagccgccc
     7801 gagcttgtgc gggttgcgga tcgcgaagat ctgcgtgatc cggccgttct cgatgacaaa
     7861 cgacacggcc gtatcgttct cgccacccgg gtcgatgcgc gctgcgatcc cgccgttgag
     7921 ctcgacgatg aacgccttcg ctccaggcgc gagcttcgca aagctggcca tcaccggaac
     7981 cagtttcgcg gcgccccgca ccggcttggg gatcgtcggg gcgagtccgc cgccgtcgcc
     8041 gacgacgagc acgtcgggtg cgagcacctt gagcaggccc ggcacgtcgc cggtggtcag
     8101 ggcggcgagg aaccgttcga cgacctggtc ctgctcggtg cggctcaccg acatgcgcgg
     8161 gcggcgagcg gccacatgct tgcgggcgcg tacggcgatc tgccgcaccg ccgcgggcga
     8221 cttgtccagg gcctcggcga tctcgtcata cggcacgtcg aagacctcac gcagcacgaa
     8281 gaccgcgcgc tccgtcggca gcagggtctc gagaaccgcg agcacggcga tcgacacgct
     8341 ctctgcgagc tcgacgtcct cggccacgtc ggggctggtc agcagcggct cgggcagcca
     8401 ctgcccgacg tagtcctcac gctgccttgc cagcgtacgt agccggttca gcgatagccg
     8461 ggtcacgatc ctcaccaggt aggcgcgcgc cttatgcacc tccgcacgct cggtctcgtc
     8521 tagggaggcc caccgcagcc aggtctcctg caccacgtcc tcggcgtcgc tggccgagcc
     8581 gagcatctcg taggcgacgg tgaagagcag gctgcggtgg gcgaggaagg ggtcctcgct
     8641 catgacgcgg acgctacgcc gggtcgctcg gccagcggct tcatcccgca ggccgccgcg
     8701 aagccgtccg actcgatgcc gagggcgacg ttgccgcgcg tggtcaggtt ggcgaatccg
     8761 atcacggacg tgagctcgac gaccgcggcc ggcccgagct gcgcgttcag gcggtcgacc
     8821 agttcgccgg tcacggtcgg cggcgtctgg ctcatcgcct cggcgtactc caggacgtct
     8881 cgctccagcg gcgtgaatac gtccgactca cgccaccgcg gaatctcacg tgccttgtcg
     8941 acgtcgaggc cccggttgcg cacctcgaag tagttgaagt ccaaacacca ggtgcagccg
     9001 accagcgagg caacggccat atgggcgaac gacttcagac tctcgtcgca ggcgtcccac
     9061 ttctcgagct tgccaccgaa gccgaaactg gcgaagagca ccctggggtt gtgccagtac
     9121 acgctcacgg acgtcggcac cttgcccagc ctcttcagga tcatccgctt gaccagcgcc
     9181 cccttga
//
LOCUS       DATIBB010000071         5447 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_1614286, whole genome shotgun sequence.
ACCESSION   DATIBB010000071 DATIBB010000000
VERSION     DATIBB010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 5447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5447
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1614286"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..265
                     /locus_tag="VLC50_02510"
     CDS             <1..265
                     /locus_tag="VLC50_02510"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HSF26369.1"
                     /translation="ASVSSCFALAVAWSARRLGVDIGEPGDLTVTAVGEYDGASYAAI
                     TITVATGSREPAELDRVLKEAQRVCYVTNTLRRAPDLTFTLAP"
     gene            complement(383..1237)
                     /locus_tag="VLC50_02515"
     CDS             complement(383..1237)
                     /locus_tag="VLC50_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006917634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HSF26370.1"
                     /translation="MDEKVKGRVIDAHSHIGTMDAWKFYNLKEPVKPTVYEFDSAKAY
                     LDQMDSVGVERGLILPNYGIPVQSQPFSLNDLVLESIANNDRLVGGLWVSFLPQNKEM
                     TLQTLKLAGERGIKALKTTFLLGGNPDPNTWDDDTREIAEACIDAVERHNLVFHFHTS
                     AGGTSDINNYIPLVEKYGKRIKIYLVHFGGGVSGHIKLVPQFLDWVEQGYKVYCDTTW
                     TIGFGARWLLTEIEKRGVGEDRVLFASDEPWSDFWSEYYKIYGAPVGDELKARILYQN
                     YEALYGEK"
     gene            1345..2322
                     /locus_tag="VLC50_02520"
     CDS             1345..2322
                     /locus_tag="VLC50_02520"
                     /inference="COORDINATES: protein motif:HMM:NF012795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HSF26371.1"
                     /translation="MPASGTLEQLAAQVRAHPGLTAKSDIGLVTEVLGPTDWLRGPGD
                     DGAAVPALGSHVVACGEALLPAFVRADPYGAGVAAVLTNLNDLAAMGAEPLGIVDTIV
                     GDEATARAALRGMRMASELYGVPILGGHLTRSDTERAISAFGVGTARHPLSMTHVAAG
                     QSIVVAAAVEGQMRADFPFFRSFEARGDRCAADLRVLAEVADSGAAVAAKDISMAGVV
                     GSLAMLLEWGGYGAELDLDRLPAPTGVELARWLVAFPSFGFLLCAPPGREVDCLEPFR
                     RRGLCAEVVGRVDGTGQVRLRRAGQTVPVVDVRAGVTRLHGPTPATPTS"
     gene            2476..>5447
                     /locus_tag="VLC50_02525"
     CDS             2476..>5447
                     /locus_tag="VLC50_02525"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HSF26372.1"
                     /translation="MDSVRLALRLAGDFKVSGNLLAPLPAGKAVRLLKFLTAARGRFV
                     ATEEIIDALWLDEPPNKAERNIAALVSRLRRSLGADRIEGGPSGYRLVLDDNVDVDLF
                     AAEAHLVDAEHHLGRAMYGVAVTAAEAAEQVLGGGAALADEPDAAWVDKLRQRASTLL
                     RRARACRWAAALAMGDAATAVDAATAALADDPVDEEATRALMRAHRSRGDDAAALIAY
                     ETLRTTLRDWLGADPSVATRDLYVAVLRNDPVPEPDRHEKPLLPGPSTLLDRAHELDH
                     LRGLWSRAAAGSGGVVVVAGQDGIGKSALADALADEARHSGALVLSTRCFEVERSLYL
                     QPLVEVIRTVVGHSSAAMMRDAAAGWLGTLTELVPELGLLVGQVPYERAEPEFEHHRS
                     LNALVSFFHRLTETRPVLLVVDDFEHAGQSTVEALQFLATRLAHWRMLVVVAERSGVS
                     EPAGARFGSMATTLELGPLSRAAVKAMADRTSLDYDIDELYEWTSGSPLFVTELLRQN
                     DGRATLAIPESLHDAVRDRMTQAGDNVERLLQHGSILGRSFTLETVAALSERTVEDCA
                     AAADRALRAGLLTTAGGTFQFANDVVRQVAYGSVLEPVRISRHRRAATVLADRPEAAA
                     YQLVASQDWRPAALAWLQAADSARLGFGTIDADRLLSQALACAERSEDDRLVAEVHIH
                     RGQVRCERAQHMLARDDHEAALKIALALGDEELEARVLELLGWTALFARDAMAAVELA
                     SRARHLAETAVAAPAAMPGALLLLGRVRHWDGDYAGADDAYAEALTSGLDDSTRAMAL
                     SYQGALLAHRDQYAAAQETLERAVRLCRRTGAFRPLLQSLFFTALARGNGGDLEGALS
                     ALHSARGLLDEYGVDLYRAGIETNLSWLWRELGEPHRAREHADRAVELSRRGGGALEL
                     EQELHAQLALADCDLLDGREDDAAHHVEAAAALLAVPLPFRHRATMRLLEMRARWETG
                     AAEELLETARSYSSPKYAA"
BASE COUNT          713 a         1991 c         1951 g          792 t
ORIGIN      
        1 ggcctcggtg tcctcctgct tcgcgctggc ggtggcctgg agcgcccggc ggctcggtgt
       61 cgacatcggc gagccgggcg acctgacggt caccgcggtc ggcgagtacg acggggcgtc
      121 ctacgccgcc atcacgatca ccgtggcgac cggcagtcgc gagccggccg agctcgaccg
      181 ggtgctcaag gaggcccagc gggtctgtta cgtcaccaac acgctgcgcc gggcccccga
      241 cctcaccttc accctcgccc cctgatggcc cgccaccccg gcgaatgacc acgccaagta
      301 ggcgtcctgg cacttggcaa ggcgtgcacg ccctgccaag tgccagaacg ccttgccaag
      361 cgcgacgagg cgacgctagc cgctacttct cgccgtacag ggcctcgtag ttctggtaga
      421 ggatgcgggc cttgagctcg tcgccgaccg gggcgccgta gatcttgtag tactcgctcc
      481 agaagtcgct ccacggctcg tcggaggcga acagcacccg gtcctcgccg acgccgcgct
      541 tctcgatctc ggtgagcagc cagcgggcgc cgaagccgat cgtccaggtg gtgtcgcagt
      601 agaccttgta gccctgctcg acccagtcga ggaactgcgg gaccagcttg atgtgcccgc
      661 tcaccccgcc gccgaagtgg acgaggtaga tcttgatccg cttgccgtac ttctccacca
      721 gcgggatgta gttgttgatg tcggaggtgc cgccggcgct ggtgtggaag tggaacacca
      781 ggttgtgccg ctcgaccgcg tcgatgcagg cctcggcgat ctcgcgggtg tcgtcgtccc
      841 aggtgttggg gtcggggttg ccgccgagca ggaacgtggt cttgagcgcc ttgatgccac
      901 gctcaccggc cagcttgagc gtctgcagcg tcatctcctt gttctgcggg aggaacgaca
      961 cccacagccc accgacgagc cggtcgttgt tggcgatgct ctcgagcacc aggtcgttga
     1021 gggagaacgg ctggctctgc accgggatcc cgtagttggg caggatcagg ccccgctcca
     1081 ccccgacgga gtccatctgg tcgagatagg ccttggcgct gtcaaactcg tagacggtcg
     1141 gcttcaccgg ctccttgagg ttgtagaact tccatgcgtc catcgtgccg atgtggctgt
     1201 gcgcatcgat gacgcgaccc ttgaccttct cgtccatggc cacgctcgcc ctctcccttc
     1261 acagatttac tatgcgactc gcacacgtac gctataggaa acgatcggcg ccgagaagac
     1321 tcgcgcccgc ccaggaggcc atccatgccg gctagcggca cgctcgagca gctggccgct
     1381 caggtgcggg cccaccccgg cctgaccgcg aagtcggaca tcgggctggt caccgaggtg
     1441 ctcggcccga ccgactggct gcgcgggccc ggcgacgacg gcgctgcggt gcccgcactc
     1501 ggctcccacg tggtggcctg cggggaggcg ctgctgccgg cgttcgtgcg ggccgacccg
     1561 tacggcgccg gggtggccgc cgtactcacc aatctcaacg acctggccgc gatgggcgcc
     1621 gaaccgctgg gcatcgtcga cacgatcgtc ggcgacgagg ccaccgcccg ggcggcgctg
     1681 cgcggcatgc ggatggcctc cgagctctac ggcgtaccga tcttgggcgg gcacctcacc
     1741 cgctcggaca cagagcgagc gatctccgcg ttcggcgtgg gcaccgcccg gcacccgctg
     1801 tcgatgaccc acgtggcggc ggggcagagc atcgtcgtgg ccgccgcggt cgaggggcag
     1861 atgcgggccg acttcccgtt cttccgctcg ttcgaggccc gcggcgaccg gtgtgccgcc
     1921 gacttgcgag tgctcgccga ggtggccgac tcgggcgccg ccgtggcggc caaggacatc
     1981 agcatggccg gggtggtcgg ctcgctggcc atgctgctcg agtggggtgg gtacggcgcc
     2041 gagctcgacc tcgaccggct gcccgccccg accggggtcg agctggcccg ctggctggtc
     2101 gcattcccga gctttggctt cctgctctgc gccccgcccg gccgggaggt cgactgcctc
     2161 gagccgttcc gccgccgcgg gctgtgcgcc gaggtggtcg ggcgggtcga cggcaccggc
     2221 caggtgcggc tgcgccgagc cgggcagacc gtgccggtcg tcgacgtccg ggccggggtc
     2281 acccggctgc acgggcccac cccagccacc cccacctcgt gatcgacgta catcgattgc
     2341 ttgatcaccg gcatgggatg tgcacaaatt gcttgatcac cggcatgggg tgcgcgcgga
     2401 ttgcttgatc accgacagag ggagtgacgc gcgaagatcg gcgtcatgca ggggtgccct
     2461 gggtagggtc agattgtgga ctctgtccgg cttgccctga ggttggccgg agacttcaag
     2521 gtctctggca atctgctggc gccgctgccg gcgggcaagg ccgtgcggct gctcaagttc
     2581 ctcaccgcgg cccgggggcg ctttgtcgcc accgaagaga tcatcgacgc gctctggctc
     2641 gacgagccgc ccaacaaggc cgagcgcaac atcgccgcgc tggtcagccg gctgcgccgc
     2701 tcgctcggcg ccgaccgcat cgagggcggc ccgagcggct accgcctggt gctcgacgac
     2761 aacgtcgacg tggacctgtt tgccgccgag gcccacctcg tcgacgccga gcaccacctc
     2821 ggacgggcga tgtacggcgt ggcggtgacc gcggccgagg cggccgagca ggtcctcggc
     2881 ggcggggcgg cgctggccga cgagcccgac gccgcctggg tcgacaagct gcgccagcgc
     2941 gcgagcacgt tgctgcgccg ggcccgcgcc tgccgctggg ccgccgccct ggcgatgggc
     3001 gacgccgcca ccgcggtcga tgcggccacg gccgcgctgg ccgacgaccc ggtcgacgag
     3061 gaggccaccc gggcgttgat gcgggcccac cgcagccgcg gcgacgacgc cgccgcattg
     3121 atcgcctacg agacgctgcg caccaccctg cgcgactggc tgggcgccga cccctcggtg
     3181 gcgacccgcg acctctacgt cgccgtgctc cgcaacgacc cggtgcccga gcccgaccgg
     3241 cacgagaagc cgctgctgcc gggcccgagc acgctgctcg accgggccca cgagctcgac
     3301 cacctgcgcg ggctgtggtc gcgggcggcg gccgggtccg gcggcgtggt cgtcgtggcc
     3361 ggccaggacg gcatcggcaa gagcgcgctg gccgacgcgc tggccgacga ggcccgccac
     3421 agcggcgcgc tcgtgctgtc cacccgctgc ttcgaggtcg agcgctcgct ctacctgcag
     3481 ccgctggtcg aggtgatccg cacagtggtc ggtcactcgt cggcggccat gatgcgtgac
     3541 gcggcggcgg gctggctcgg cacgctgacc gagctggtcc ccgagctggg gctgctggtc
     3601 ggccaggtgc cctatgagcg cgccgagccc gagtttgagc accatcgcag cctcaacgcg
     3661 ctggtgtcct tcttccaccg gctgacggag acccgaccgg tgctgctggt cgtcgacgac
     3721 ttcgagcacg ccgggcagtc caccgtggag gccctgcagt tcttggccac tcggctggcc
     3781 cactggcgaa tgctggtggt cgtcgccgag cgctccggag tcagcgagcc ggccggcgcc
     3841 cggttcggct cgatggccac cacgctcgag ctcggcccgc tcagccgggc cgcggtcaag
     3901 gcgatggccg accgcacctc cctcgactac gacatcgacg agctctacga gtggaccagc
     3961 gggtccccgc tgttcgtcac cgagctgctg cgccagaacg acggccgagc caccctggcc
     4021 atccccgagt cgctgcacga cgcggtccgc gaccggatga cccaggccgg cgacaacgtc
     4081 gagcgcctgc tgcagcacgg ctcgatcctc gggcgctcgt tcacgctcga gacggtggcc
     4141 gccctgagcg agcgcaccgt cgaggactgt gccgccgcgg ccgaccgggc gctgcgcgcc
     4201 ggcctgctca cgaccgccgg gggcacgttc cagttcgcca acgacgtggt gcgccaggtc
     4261 gcctacggct cggtgctcga gccggtccgg atcagccggc accggcgggc ggcgacggtg
     4321 ctcgccgacc gaccggaggc ggccgcctac cagctggtcg cctcgcagga ctggcggccg
     4381 gcggcgttgg cctggctgca ggccgccgac agcgcccggc tcggcttcgg caccatcgac
     4441 gccgaccggc tgctgagcca ggcgctggcc tgcgccgagc gctccgagga cgaccggctg
     4501 gtcgccgagg tgcacatcca ccgcggccag gtccgctgcg agcgcgccca gcacatgctg
     4561 gcccgtgacg accacgaggc cgccctcaag atcgcgctgg cgctgggcga cgaggagctc
     4621 gaggcccggg tgctcgagct gctcggctgg accgcgctgt tcgcccgcga cgcgatggcc
     4681 gcggtcgagc tggcctcccg cgcccggcac ctggccgaga cggcggtggc cgccccggcc
     4741 gcgatgcctg gcgcgctgct gctgctcggc cgggtgcgtc actgggacgg cgactacgcc
     4801 ggcgccgacg acgcctacgc cgaggcgctg acctccggcc tcgacgactc gacccgggcg
     4861 atggcgctgt cctaccaggg cgcgctgctc gcccaccgtg accagtacgc cgcagcgcag
     4921 gagaccctcg agcgcgcggt gcggctgtgc cggcgcaccg gcgcgttccg tccactgctg
     4981 cagtcgttgt tcttcaccgc gctggcccgc ggcaacggcg gcgacctgga gggtgcgctg
     5041 agcgcgctgc actcggcgcg cgggctgctc gacgagtacg gcgtcgacct ctatcgggcc
     5101 ggcatcgaga ccaacctgtc gtggctgtgg cgcgagctcg gcgagccgca ccgggcccgc
     5161 gagcacgccg accgggcggt cgagctgtcc cggcgcggcg gcggcgcgct cgagctggag
     5221 caggagctgc acgcccagct cgcgctggcc gactgcgacc tgctcgacgg acgcgaggac
     5281 gacgcggccc accacgtcga ggcggcggcc gcgctgctcg ccgtaccgct gccgttccgc
     5341 caccgcgcca ccatgcggct gctcgagatg cgcgcgcggt gggagaccgg cgcggccgag
     5401 gagctgctgg agacggcccg gtcctactcc tcgcccaagt acgccgc
//
LOCUS       DATIBB010000072         9536 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_1219970, whole genome shotgun sequence.
ACCESSION   DATIBB010000072 DATIBB010000000
VERSION     DATIBB010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 9536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9536
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1219970"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..590
                     /gene="larB"
                     /locus_tag="VLC50_02530"
     CDS             <1..590
                     /gene="larB"
                     /locus_tag="VLC50_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012917884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HSF26373.1"
                     /translation="ATRLPAPAQGAVRAAFPDAQIDPSARTAVVGPLPSPRGTVVVVA
                     AGTSDAAVAAEAATTARAFGAGVTRLDDVGVAGIHRLLAARATLDAADCLIVVAGMEG
                     ALPSVVGGLTGVPLVAVPTSVGYGASFGGLAALLGMLNACAPGVVVANIDNGFGAGVF
                     AARVARRAAPRADARATDPEAADPNGTGSDEAGAP"
     gene            587..1846
                     /gene="larC"
                     /locus_tag="VLC50_02535"
     CDS             587..1846
                     /gene="larC"
                     /locus_tag="VLC50_02535"
                     /EC_number="4.99.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014738353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HSF26374.1"
                     /translation="MTRIGWIDASVGAAGDMLAAALVGAGAPYDVMSEAVAALGVPVT
                     TAVETVEAQGLSARRWHVTTGEADPPRRTWADVRALLDAADLDPAVRALAHDAFERLA
                     TAEAAVHGTAPDAVHFHEVGAHDAIADIVGAAAGFVALRLDAVVVSPVALGGGTAHAG
                     HGLVPVPAPAVLEILRSVGAPAFGGPVDVELCTPTGAALLASVATAWGPMPPMRVETV
                     GLGAGTRTLAGRPNVVRLVVGEAADAPAGAAAAPDPGLPTQLLLETNVDDLDPRLWPA
                     VLARLLEVGVADAWLTPILMKKGRPAHTLSVLVDAEVADAARRVVFAETSTIGLRETV
                     VGKRALVRRTCSIAISGQPVRVKLAELDGAVVNAQPEYDDVVAAAAALGRPIKAVLAE
                     AAALAARAATLPGASAPAAHDAPPGAE"
     gene            1843..2409
                     /locus_tag="VLC50_02540"
     CDS             1843..2409
                     /locus_tag="VLC50_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020543381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM165/GDT1 family protein"
                     /protein_id="HSF26375.1"
                     /translation="MSAFLLSTAVIFLAELGDKSQLMAMTFATRFRARDVLIGITAAT
                     AIVHLLSVALGAAVGAALPTTAISVVAGLAFLGFAAWTLRGDRLDEDEKSKAARASSA
                     AILAVGGAFFLAELGDKTMLATITLATRENWLGTWIGSTVGMVAADALAIAVGAALGR
                     RLPEKTIRYGAAAAFAVFGVLLLATAAA"
     gene            2722..3267
                     /locus_tag="VLC50_02545"
     CDS             2722..3267
                     /locus_tag="VLC50_02545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF26376.1"
                     /translation="MLSRVSRPTALAAVLVAPLLALSLTGCGGTDGESTAAVSTTGAT
                     TATTAPATVDTSSPSAPAGPLELPDQAAALAEAFSAADYGVVWDLACPGARQNASRDE
                     FATTMSAVLTVELANPTVLDEGLTDEARADLRDPGTAYDDEALVRFDVGPGSNYPNPT
                     MTAWFVNTDTGWLYCGMVPDA"
     gene            3356..4582
                     /locus_tag="VLC50_02550"
     CDS             3356..4582
                     /locus_tag="VLC50_02550"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020390594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016990 - arginine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0019546 - arginine deiminase pathway
                     [Evidence IEA];
                     GO_process: GO:0019547 - arginine catabolic process to
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HSF26377.1"
                     /translation="MTGESTGVDSEVGRLRTVLLHRPGAELKRLTPRNNDRLLFDGIP
                     WVDRAQEEHDAFAAALRDRGVEVLYLAELLAETLVVPEARTATIKAAAPEARLGQTLT
                     RRVRAQLADLHPDDLALVLMAGLRVDELARGGGLVHRLLSHDDFVIDPLPNLLFTRDS
                     STWLRDQVAVTSLAMPARARETSLTQAIYTHHPRFAGVAQAYGPDLEHLEGGDVLLLA
                     PGVVAVGAGERTTPAGVERLARRMFARGLAHTVLAVPVAQERATMHLDTVCTMVDVDA
                     IVMYPNIAHELTAWTITVEGDPADQGGELVVEGPEPFLVAAAKAMAIDRLRVIDTGLD
                     PVTAEREQWDDGNNTLAIAPRVAVAYERNIETNARLEAAGIEVVAISGSELGSGRGGP
                     RCMSCPVARESLAGSA"
     gene            complement(4942..5898)
                     /locus_tag="VLC50_02555"
     CDS             complement(4942..5898)
                     /locus_tag="VLC50_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018892563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5926 family protein"
                     /protein_id="HSF26378.1"
                     /translation="MGKQARRRAAASSPVQTTEPVPVVGAREPCPCGSGRRYKACHGR
                     DAARAAEPMVARPFAGLPGECDWVALREFVPAATAPVRLREGTDRALTITTVLPMAWP
                     GLVRADGSTYIGLQVPGGSGDASRDAAFAIERALATEPGNPLPPVGLPGPGPRLQDLL
                     DLDAPFEVTLHEGFDFWVEGAEQTPDVVASLERANASVVPTQRLTGVEAAYWCRIGSR
                     AHLRWVMPHDEDALIDAQARLHAAGADSLGEGTRFVGSFRADGLLVPVWDLPVELAAE
                     ALEAPAVAFAQRLAEALDDTSPLTDAQRRARNGLRNRQLTLR"
     gene            complement(5987..6568)
                     /locus_tag="VLC50_02560"
     CDS             complement(5987..6568)
                     /locus_tag="VLC50_02560"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSF26379.1"
                     /translation="MSPRILPGWVRRFGEMMPPAPPLGSSSSSLTLPHDASSAREARI
                     WVMRTAVAAGMPPELVDDVALVVSELVTNAVRHARPLPGGTIEVTYSIRPTGHLVLCV
                     RDGGPRNAPPAGPGWASTTAGLEQTIAASLAASRRGDSLAMPTQGGRGLGIVGSMVDD
                     WGVDATGAATTVWAEVDPRRRGGGASSHHPLTG"
     gene            complement(6631..7467)
                     /locus_tag="VLC50_02565"
     CDS             complement(6631..7467)
                     /locus_tag="VLC50_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005482825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HSF26380.1"
                     /translation="MPASGAAAASDLRRFDIQVVAHRGSSGLLAEHTLAAYRRAIIDG
                     ADALECDVRLTADGHLVCVHDRRVDRTSNGRGIVSTLELAQLSELDWASWRGDESATM
                     AELPDVDRHGILTLRRLLETVADAGRPVEVAIETKHPTRYAGLVERRLVELLDRFGWA
                     HPRVGKTTPVRVMSFSQLSLRRLRRLAPSLPTVLLMDRTPVRYWGGALPPGVRISGPS
                     IEVLRAHPRYVERAHRMGNQVHVWTVDTPEDVIFCVDLEVDAMISNKPRLVLDVLGRL
                     GS"
     gene            complement(7666..8040)
                     /locus_tag="VLC50_02570"
     CDS             complement(7666..8040)
                     /locus_tag="VLC50_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannose isomerase type II C-terminal
                     cupin domain"
                     /protein_id="HSF26381.1"
                     /translation="MTTTDRGSDPREAIVVDERPWGRFEQLTHNEPTTVKIITVEAGH
                     RLSLQRHRYRDELWTILDGPVLVEVGARAWEAEPGEKIWVPRGTTHRVAAVGGVRGRF
                     LEIAFGEFDEGDIERLADDYAR"
     gene            complement(8089..8478)
                     /locus_tag="VLC50_02575"
     CDS             complement(8089..8478)
                     /locus_tag="VLC50_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015104404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HSF26382.1"
                     /translation="MLYLDSSALAKLVVAEAETDALLAVVARSDAVVTCALATVEVVR
                     AARLVDAALVPAARELVARLDQIPCSPDLLAEAGELQPAQLRSLDAIHLAAALRVARH
                     GLEAFVAYDRRLLSAAGSHGLPVRSPA"
     gene            complement(8483..8749)
                     /locus_tag="VLC50_02580"
     CDS             complement(8483..8749)
                     /locus_tag="VLC50_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014374056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HSF26383.1"
                     /translation="MDQVGVRELRLNVSRYLERVEAGETLEVTKRGKVIAVLAPRRTR
                     ESARDYLVATGQLVAGSGGLAAVRPVRAKRSITEALASLRADER"
     gene            complement(8830..>9536)
                     /locus_tag="VLC50_02585"
     CDS             complement(8830..>9536)
                     /locus_tag="VLC50_02585"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF012630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031012 - extracellular matrix [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HSF26384.1"
                     /translation="PGPAAAAVRLHGPPPGIDEATGPLGVPQPAPPGQGGFAFEATQA
                     DGRAPVTWDPCRPIRYVVHGTAPAGAERVVESAIAEVSAASGMVFMPVGLTDELPVTG
                     RSPYQPERYGDRWAPLVIAWSDPARTSELAGNVIGLGGGISAGWDSGHRTYVSGAVWL
                     DGPQFAEDLAHRPASERVLHAVVMHELGHALGLDHVDDPTQVMYPEARLQVTRLGDGD
                     RRGLAALWQGRCAPGL"
BASE COUNT         1282 a         3535 c         3504 g         1215 t
ORIGIN      
        1 tggccacccg gctgcccgcc ccggcccagg gcgcggtgcg cgcggcgttc cccgacgcgc
       61 agatcgaccc cagcgcccgt acggcggtgg tcggcccgct gccctccccg cgaggcaccg
      121 tggtggtggt ggccgccggc acctccgacg ccgcggtcgc cgccgaggcc gccacgacgg
      181 cgcgggcgtt cggtgccggc gtcacccgcc tcgatgacgt cggggtggcc ggcatccacc
      241 ggctgctcgc cgcccgggcc acgctcgacg ccgccgactg cctgatcgtg gtggccggca
      301 tggagggcgc gctgcccagc gtggtgggcg ggctgaccgg ggtgccgctg gtcgcggtgc
      361 ccaccagcgt gggctacggc gcgtcgttcg gcgggctggc cgcgctgctc ggcatgctca
      421 acgcctgcgc gcccggcgtc gtggtggcca acatcgacaa cgggtttggc gccggggtgt
      481 tcgccgcccg ggttgcccgt cgcgccgccc cgcgcgccga cgcccgcgcg accgatccag
      541 aggcggccga ccccaacggc accggctccg atgaggccgg cgcgccgtga cccggatcgg
      601 ctggatcgac gcctcggtcg gcgcggccgg cgacatgctc gccgccgcac tcgtcggagc
      661 cggggcgccg tacgacgtga tgagcgaggc cgtcgccgcg ctcggcgtac cggtcaccac
      721 cgccgtcgaa accgttgagg cgcaagggct ttcggcgcgg cgctggcacg taaccaccgg
      781 cgaggccgac ccgccgcggc gcacctgggc cgacgtgcgg gccctgctcg acgccgccga
      841 cctcgacccg gcggtgcggg cgctggcgca cgacgcgttt gagcggctgg ccaccgccga
      901 ggccgcggtg cacggcacgg cgccggatgc ggtgcacttc cacgaggtgg gcgcgcacga
      961 cgcgatcgcc gacatcgtgg gcgctgccgc cgggttcgtc gcgctgcgcc tggacgccgt
     1021 ggtggtctcg ccggtcgccc tcggcggtgg caccgcccac gccggacacg ggctggtccc
     1081 ggtgccggcc ccggcggtgc tggagatcct gcggtcggtg ggcgccccgg cgttcggcgg
     1141 gccggtcgac gtcgagctgt gcaccccgac cggcgcggcg ctgctggcat cggtggccac
     1201 cgcgtggggg ccgatgccgc cgatgcgggt cgagacggtc gggctgggcg ccggcacccg
     1261 cacgctggcc gggcggccca acgtggtgcg gctggtggtc ggcgaggcgg ccgacgcccc
     1321 agccggcgcc gccgcggccc ctgaccccgg cctgcccacc cagctgctgc tcgagaccaa
     1381 cgtcgatgac ctcgacccgc ggctgtggcc ggcggtgctg gcccggctgc tcgaggtcgg
     1441 ggtggccgac gcctggctga ccccgatcct gatgaagaag ggccggcccg cgcacaccct
     1501 gtcggtgctc gtcgacgccg aggtggccga cgcggcccgg cgggtggtct tcgcggagac
     1561 ctcgacgatc gggctgcgcg agaccgtggt cggcaagcgc gcgctggtcc gtcgtacctg
     1621 ctcgatcgcc atctccggcc aaccggtgcg ggtcaagctg gccgagctcg acggggccgt
     1681 ggtcaacgcc cagcccgagt acgacgacgt ggtggcggcc gccgccgccc tgggccggcc
     1741 gatcaaggcg gtgctcgccg aggcggccgc gctggctgcc cgcgccgcga ccctgcctgg
     1801 ggcgtctgcg ccggccgctc acgatgcacc gccgggagcc gagtgagcgc gttcctgctg
     1861 agcactgccg tgatcttcct cgccgagctg ggcgacaaga gccagctcat ggcaatgacg
     1921 tttgccactc ggttccgcgc ccgcgacgtg ctgatcggca tcacggcggc caccgcgatc
     1981 gtgcacctgc tctcggtcgc tcttggtgcg gcggtcggcg cggcgctgcc cacgacggcg
     2041 atcagcgtcg tggcggggct ggcgttcctc gggttcgcgg cctggaccct gcgcggtgac
     2101 cgcctcgacg aggacgagaa gtcgaaggcc gcccgggcca gctcagccgc gatcctggcc
     2161 gtcggcggcg cgttcttcct ggccgagctc ggcgacaaga cgatgctcgc caccatcacg
     2221 ctggccaccc gggaaaactg gctgggcacc tggatcggca gcaccgtggg catggtggcc
     2281 gccgatgcgc tggctatcgc ggtcggcgcc gcgctggggc ggcgactgcc cgagaagacg
     2341 atccgctacg gcgcggccgc ggcattcgcc gtcttcggcg tactcctgct cgccaccgcc
     2401 gccgcctgac cccgccacac gcccctgccc gcccctcccc tgcactgatc atgcgcgttt
     2461 ggcggccccc gcccccctgc actgatcatg cgcaaaccat cgcgcgccgc gagacgaagc
     2521 aacgccctct gacccctggc gcgatcgtga tgcatccgcg tcgtggacac accgccaacg
     2581 catcatgatc ggccgcgacc gagccggccg gcgtaaggcg gcgacggtca agtgggcgac
     2641 gacacgtgtc gatggcacag tgagccttgt ccgatctgag atgtttgacc ggttggcgtc
     2701 cgtcgtaccg agaggaccct cgtgctttcc cgcgtctccc gtcccaccgc gctggccgcc
     2761 gtgctggtcg cgcccctgct cgccctgagc ctgaccggct gcggcggaac cgacggcgag
     2821 agcaccgccg ccgtgagcac caccggcgcc accaccgcaa ccaccgcccc cgccaccgtc
     2881 gacacctcga gcccgtcggc gccggccggc ccgctcgagc tgcccgacca ggcggccgca
     2941 ctcgccgagg cgttcagcgc cgccgactac ggggtggtct gggacctggc ctgcccgggc
     3001 gcccggcaga acgcgagccg cgacgagttc gccaccacga tgagcgctgt cctcacggtc
     3061 gagctggcca acccgacggt gctcgacgag ggcctcaccg acgaggcgcg cgcggacctg
     3121 cgtgaccccg gaacggcgta cgacgacgag gcgctggtcc ggttcgacgt gggcccgggc
     3181 agcaactatc ccaacccgac catgaccgcc tggttcgtca acaccgacac cggctggctc
     3241 tactgcggga tggtccccga cgcctgatcc acccttgcga ccccgcgacc cgcgggaccc
     3301 tccgccagac gcgcatgatc agtgcagagg ggggccggcg actagggtcg cggccatgac
     3361 cggggaatcc acgggggtcg acagcgaggt cggccggctg cgtaccgtct tgctgcatcg
     3421 cccgggcgcc gagctcaaac ggctgacccc gcgcaacaac gaccggctgc ttttcgacgg
     3481 catcccgtgg gtcgaccgcg cccaggagga gcacgacgcg ttcgccgcgg ccctgcgcga
     3541 ccgcggcgtc gaggtgctct acctcgcgga gctgctggcc gagacgctcg tcgtacccga
     3601 ggctcgcact gcgaccatca aagccgccgc gccggaagcc cggctcgggc agacgctgac
     3661 ccgccgggta cgcgcccagc tggccgacct gcaccccgac gacctcgcgc tcgtgctgat
     3721 ggccgggctg cgggtcgacg agctggcccg cggcggcgga ctcgtgcacc ggctgctcag
     3781 ccacgacgac ttcgtcatcg acccgctgcc caacctgctg ttcacccgcg actcgagcac
     3841 ctggctgcgc gatcaggtcg cggtgaccag cctggcgatg ccggcccggg cccgggagac
     3901 ctcgctgacc caggcgatct acacccacca cccgcggttc gccggggttg cgcaggccta
     3961 cggccccgac ctcgagcacc tggaaggcgg cgacgtgctg ctgctcgcgc ccggggtggt
     4021 ggcggtcgga gcaggcgagc gtacgacgcc ggccggggtg gagcggctgg cgcggcggat
     4081 gttcgcccgc gggctggccc acaccgtgct cgccgtaccg gtggctcagg agcgggccac
     4141 catgcacctg gacaccgtgt gcaccatggt cgacgtcgac gcgatcgtca tgtatcccaa
     4201 catcgcccac gagctgaccg cctggacgat caccgtggag ggcgacccgg cggaccaggg
     4261 cggcgagctg gtggtcgagg ggcccgaacc gttcctcgtg gccgcggcca aggcgatggc
     4321 gatcgaccgg ctgcgcgtga tcgacaccgg gctcgacccg gtcaccgccg agcgcgagca
     4381 gtgggacgac ggcaacaaca cgctggcgat cgccccgcgg gtggcggtgg cctacgaacg
     4441 caacatcgag accaacgctc ggctggaggc ggccggcatc gaggtcgtgg cgatcagcgg
     4501 gagcgagctc ggcagcgggc ggggcgggcc gcggtgcatg tcgtgcccgg tggcgcggga
     4561 gtctctcgcc ggctccgctt gaccgacctc gacgtgcagg ggcgcgaaac cgggcctagc
     4621 ggacatcggc cccagacaaa tggatggccc ggacgctgct gacgggggat gcagcaacgt
     4681 ccgggcttga gacaaagagt agcggatcct gcgggaacac caacaccagg gtccagactt
     4741 tgacaaggct tgacctaaca gccccattcc gtccgctctg ccgacgccgg cgcccggccg
     4801 gtgcatagtg ccggacacaa aggacggaac aagccgtcga aacagccgga tgcgccgtcg
     4861 ttcggcgcgt tgttccgtcc ctagtgtccg caatgacggg gctgcgccgg ggggtcagcg
     4921 cgggcggggc ggcgcaggcg gtcagcgcag ggtgagctgc cggttgcgca ggccgttgcg
     4981 ggcccggcgt tgggcatcgg tgagcggcga ggtgtcgtcc agcgcctcgg ccagccgctg
     5041 ggcgaaggcg accgccggtg cctccagcgc ctcggcagcc agctcgaccg gcagatccca
     5101 gaccgggacc agcagcccgt cggcccggaa cgacccgacg aaccgggtgc cctcgcccaa
     5161 cgagtcggcg ccggcggcgt gcagccgggc ctgggcgtcg atcagcgcgt cctcgtcgtg
     5221 gggcatcacc cagcgcaggt gggcgcggct gccgatccgg caccagtacg ccgcctccac
     5281 gccggtcaac cgctgggtcg ggaccacgct ggcgttggcc cgctcgaggg aggccaccac
     5341 gtcgggggtc tgctcggcgc cctcgaccca gaagtcgaag ccctcatgca acgtcacctc
     5401 aaacggcgcg tccaggtcga gcaggtcctg cagccgcggg ccggggccgg gcagcccgac
     5461 cgggggcagc gggttgcccg gctcggtggc cagcgcccgc tcgatcgcga aggccgcgtc
     5521 acggctggcg tcgccggacc caccgggcac ctgcagcccg atgtacgtcg acccgtcggc
     5581 gcggaccaac ccgggccagg ccatcggcag cacggtcgtg atggtcagcg cccggtcggt
     5641 tccctcacgc agccgcaccg gcgcggtggc cgccggcacg aactcgcgca gcgcgaccca
     5701 gtcgcactcc ccgggcagcc cggcgaacgg gcgagccacc atcggttcgg ccgcgcgcgc
     5761 ggcgtctcgg ccgtgacagg ccttgtagcg gcgacccgat ccgcacggac acggttcgcg
     5821 ggccccgacg actgggaccg gctcagtcgt ctggacggga gatgaggcgg cggcgcggcg
     5881 gcgggcctgc ttgcccatgg cggagcactc cgggggcagg gggacgggac ccgggcagcc
     5941 tagcgacctt gccgaccggc cgcggcgcac gcgatcgcgg gcgggctcag cccgtgagcg
     6001 ggtggtgcga ggaggcgccg ccgccccgac gacggggatc gacctcggcc cacaccgtgg
     6061 tggccgcacc cgtggcgtcg acgccccagt cgtcgaccat gctgccgacg atgcccagcc
     6121 cgcgcccgcc ctgcgtgggc atggccaacg agtccccccg ccgagacgcg gcgagggagg
     6181 ccgcgatggt ctgctcgagc ccggcggtgg tcgaggccca accgggcccg gcgggcggtg
     6241 cgttgcgggg tccgccatcg cgcacgcaga gcacgaggtg gccggtcggc cggatcgagt
     6301 aggtcacctc gatcgtgccc ccgggcagcg gtcgggcatg acgcaccgca ttggtgacga
     6361 gctcggagac cacgagcgcg acgtcgtcga caagctccgg cggcattccg gcggcgactg
     6421 ccgtacgcat cacccaaatg cgggcttcac gggcgctcga cgcgtcgtgc ggcagcgtca
     6481 gcgacgagga agaactgccg agtggcggcg cggggggcat catttctccg aagcgtcgga
     6541 cccagccagg aaggattcgc gggctcatct tccataaggc tgcccgttgt caagacccgt
     6601 gaaacagccg gccgttgtgt ggtcctccgg tcaggagccc aaacggccga gcacgtcgag
     6661 cacaaggcgt ggcttgttgc tgatcatcgc atctacctcg agatcaacac aaaatatgac
     6721 atcttccggc gtgtctaccg tccagacgtg cacctggttg cccatccggt gcgcccgttc
     6781 gacgtaccgg ggatgggccc gcagcacctc gatgctcgga ccggaaattc gtacgccggg
     6841 cggcagcgca ccgccccagt agcggaccgg cgtgcggtcc atgagcaaca ccgtcggcag
     6901 gctcggggcg agccggcgca gtcggcgcaa cgagagctgg gaaaagctca tcacccgcac
     6961 cggcgtggtc ttgccgaccc gggggtgggc ccagccgaac cggtcgagca gctcgaccag
     7021 ccggcgttcg accaggccgg cgtagcgggt cgggtgcttg gtctcgatgg ccacctcgac
     7081 cgggcggccg gcgtcggcga ccgtctcgag cagccggcgc agggtgagga tgccgtgccg
     7141 gtcgacgtcg ggcagctcgg ccatggtcgc ggactcgtca ccccgccacg acgcccagtc
     7201 gagctcgctc aactgggcca gctcgagcgt ggagacgatg ccccggccgt tggaggtgcg
     7261 gtcgacccgg cggtcgtgca cgcagacgag gtgcccgtcg gcggtgagcc gcacgtcgca
     7321 ctcgagcgcg tcggccccgt cgatgatcgc ccggcggtag gcggccaggg tgtgctcggc
     7381 cagcaggccg gaggagccgc ggtgggcgac cacctggatg tcgaaccgcc gtaggtcgga
     7441 ggcggcagcg gcaccggacg cgggcacccg ctcatcgtgg cacgcacgcc tgcgcccacc
     7501 gcatcacccc tgcggcgtgc ccgcgcgcgg cgggtcaccg gtgcagggcg tacacaaagg
     7561 acggaacaac cccggcgtac ggcgcccgct cgtgctcagc gtcgcggcga cgaggccgtt
     7621 gttccgtccc tagcgtccag ggcagtccgc agagggcggg cggggtcagc gggcgtagtc
     7681 gtcggcgagg cgttcgatgt cgccctcgtc gaactcgccg aacgcgatct ccaggaaccg
     7741 cccgcgcaca ccgccgacgg cggccacccg gtgcgtcgta cccctgggga cccagatctt
     7801 ctcgcccggc tcggcttccc aggcccgcgc gccgacctcg accagcaccg gcccgtcgag
     7861 gatcgtccac agctcgtcgc ggtagcggtg ccgctgcagg ctcagccggt gcccggcctc
     7921 cacggtgatg atcttgacgg tggtcggctc gttgtgggtc agctgctcga accgccccca
     7981 cgggcgttcg tcgacgacga tcgcctcccg agggtcactc cctcgatccg tggtggtcac
     8041 ggcgccaggc taaccagagc cgcgctcctt caccggcccg acaccagatc acgccggcga
     8101 gcgcaccggc agcccgtgag agccggcggc ggacaggagg cgccggtcat aggccacgaa
     8161 tgcctccaga ccatggcggg ccacccgcag cgccgcggcc aggtggatgg cgtccaacga
     8221 gcggagctgg gcaggctgga gctcacccgc ttcggcgagc aggtcggggc tgcacgggat
     8281 ctggtcgagg cgtgccacca gttcgcgcgc ggccggcacg agagccgcgt cgacgagccg
     8341 ggcggcgcgc accacttcaa cagtcgccag cgcacaggtg accacggcgt cgctgcgcgc
     8401 caccaccgcg agcagcgcgt cggtctccgc ctcggccacg acgagcttcg ccagcgccga
     8461 cgagtcgagg tagagcacca gctcagcgct cgtcggcgcg cagcgatgcc agcgcttcgg
     8521 tgatcgagcg cttcgctcga acggggcgta cggcggcgag cccgccgctg cccgcgacca
     8581 gctgaccggt ggcgacgagg tagtcgcgag cgctctcccg ggtgcgtcgc ggcgcgagta
     8641 ctgcgatcac cttgccgcgc ttggtgacct ccagggtctc ccccgcctcg acccgctcga
     8701 ggtagcggct gacgttgagc cgcagctccc gcacgcccac ctggtccatg tgcaacatgg
     8761 tactacttct ggtgatcgag cgacgcgagc gcagcgtgcc gatcaagcgg cgggagtgca
     8821 gcgttccgcc tagaggccgg gggcgcagcg gccctgccag agcgcggcca gcccgcggcg
     8881 gtcgccgtcg ccgagccggg tcacctgcag ccgggcctcc ggatacatca cctgggtcgg
     8941 gtcgtcgacg tggtcgaggc cgagcgcgtg gcccagctcg tgcatcacca cggcgtgcag
     9001 cacccgctcg ctggccggcc ggtgggccag gtcttcggcg aactgcggcc cgtcgagcca
     9061 caccgcgccg ctgacgtagg tgcggtgtcc gctgtcccag ccggccgaga tgccgccgcc
     9121 cagcccgatc acgttgccgg ccagctcgga cgtgcgggcc gggtcgctcc acgcgatgac
     9181 caggggcgcc cagcggtcgc cgtaccgctc gggttggtag ggcgagcgcc cggtgaccgg
     9241 cagctcgtcg gtgagcccga ccggcatgaa caccatgccc gatgccgccg acacctcggc
     9301 gatcgccgac tcgaccaccc gctcggcgcc ggccggggcg gtgccgtgga ccacgtagcg
     9361 gatcggccgg cacgggtccc aggtgaccgg cgcgcgcccg tcggcctggg tcgcctcgaa
     9421 cgcgaagccg ccctgcccgg gaggcgccgg ctggggcacc ccgagcgggc cggtggcctc
     9481 gtcgatgccg ggtggcgggc cgtgcaggcg tacggcggcc gcggcgggac cgggct
//
LOCUS       DATIBB010000073         4260 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_1747162, whole genome shotgun sequence.
ACCESSION   DATIBB010000073 DATIBB010000000
VERSION     DATIBB010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 4260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4260
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1747162"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1988)
                     /locus_tag="VLC50_02590"
     CDS             complement(<1..1988)
                     /locus_tag="VLC50_02590"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HSF26385.1"
                     /translation="MLVVPGVSAPAQASVTQAARTATATAAASPTDTAVFFAADGLRQ
                     DLVAQYAAEGELPTMAEMLKKGTSASDAGLLTEAPPNTGAGWYSLATGAWPAVHGSTN
                     NTFHISGQPFGNRTAAFDPGVLQAETIAQAAERGGKRVAQVEWAGGRNGEIDGPTIDF
                     LSFFSGRGVATNYISPSDNAQFVASFGLQFDHPAGFAGQPAFAAAAPTDATEWSNVPT
                     SFSPAKQMRLRVIDAGQDKYGLNAYLYDSTDNGTVDYDRVLFATGKDGDAKVADLREG
                     ELADVKVQIVGGTLSGKTAGFLLKVERLTPDLSQVRLFHTSVSRANATWAGWPGDPAY
                     PSATDFAEYLAQRFPTSTAADFAVLEAGIVSEETYVQQGLYWETGHQPMLRYVVETYH
                     PDLLLAGYPTTDEFQHQFLGLVSKTLPNGAPNPAYDDVEVNGTPDGRVSEREAFIERA
                     YQGADQTLSLARSLMGEDPVTFVASDHGFAPQFLAIDASKVLVDLGLLSKPQTSNCRP
                     ATGETIGKAKACWAGGTVQVYLNLAGRDPAGGGFTQVAAADEATTVAAIKAAFLALSD
                     PNDWTGDGQPEGWKVIDRAFTKAEARYIPNGPGSTADMAHPTRTGDLVAFAYPPYQFD
                     AATPGTLIARSAFFGQHGYVPDIKDADAATSMRATFLAG"
     gene            complement(2193..2870)
                     /locus_tag="VLC50_02595"
     CDS             complement(2193..2870)
                     /locus_tag="VLC50_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020544660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HSF26386.1"
                     /translation="MLVVEDEASFADALGYMLEKEGFEVVHAASGPDALAVFDRSEID
                     LVLLDLMLPGLPGTEVCRALRQRSTVPIIMLTARDSETDKVVGLELGADDYVTKPFST
                     RELIARIRAVLRRRDEHHEASYDDGVLAAGPIRMDVDRHTVTVSGAEITLPLKEFDLL
                     ELLLRNVGRALTRDQLITRVWGSDYVGDTKTLDVHVKRLRSKIEAEPGSPKLLLTIRG
                     LGYKLEG"
     gene            complement(2879..4051)
                     /locus_tag="VLC50_02600"
     CDS             complement(2879..4051)
                     /locus_tag="VLC50_02600"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSF26387.1"
                     /translation="MEALLAVLVAGVAALVLGWTWRTRLRSVRRRDSTVLDTAVLREL
                     RLGVVVVDAAGDAVLANDAARAMGLLRGQALSIPELRTLADKTRRTGHRHEQQVQLLH
                     GWLPRDPVAVLAKAAPADRAGQVVLVVEDITEAHRLAEMRRDFVINVSHELKTPVGAL
                     ALLAEALQAASDDPDAVRRFAGRIEHESVRLGRLVSELIDLSRLEGAEPLPEPTPVRV
                     GQVVAEAADRAQAMAEMRSVSLVADGDLDATILGNRAQLVTAVANLLDNAIRYSSDGM
                     TVSTTVRRKPGGQGVDLDAAAAVEIAVTDTGVGIAEEDLERVFERFYRVDKARSGSGA
                     GTGLGLAIVKHVAANHGGRVGVFSRVRSGSTFTITLPAAPMLEDRSIQAPTQEASA"
BASE COUNT          596 a         1434 c         1559 g          671 t
ORIGIN      
        1 ccggccagga aggtcgcccg catgctcgtg gccgcgtcgg cgtccttgat gtcggggacg
       61 tagccgtgct ggccgaagaa cgccgaccgg gcgatcagcg tgccgggcgt ggccgcgtcg
      121 aactggtagg gcgggtaggc gaacgcgacc aggtcgcccg tgcgggtggg gtgggccatg
      181 tcggcggtgg agcctggccc gttgggaatg taacgcgcct cggccttggt gaatgcgcgg
      241 tcgatgacct tccagccctc gggctggccg tcaccggtcc agtcgttggg gtcggacagc
      301 gcgaggaacg ccgccttgat cgcggccacg gtcgttgcct cgtcggcggc ggccacctgc
      361 gtgaacccgc cgccggccgg gtcccggccg gccaggttga ggtagacctg cacggtgccg
      421 ccggcccagc acgccttggc cttgccgatg gtctcaccgg tcgccgggcg gcagttggag
      481 gtctgcggct tgctcagcag gccgaggtcg acgagcacct tgctcgcgtc gatggcgagg
      541 aactgcggcg cgaaaccgtg gtcagaggcc acgaacgtga ccgggtcctc gcccatcaac
      601 gaccgcgcca ggctcagtgt ctgatcggcg ccttggtagg cgcgctcgat gaacgcttcg
      661 cgctcggaca cgcggccgtc cggggtgccg ttgacctcga cgtcgtcgta ggccgggttg
      721 ggggcgccgt tgggcagcgt cttggagacc aggccgagga actggtgctg gaactcgtcg
      781 gtcgtggggt aaccggccag cagcaggtcg gggtggtagg tctccacgac gtagcgcagc
      841 atcggctggt ggccggtctc ccagtagaga ccctgctgca cgtaggtctc ctcgctgacg
      901 atgccggcct cgagcaccgc gaagtcggcc gcggtcgagg tcgggaagcg ctgcgcgagg
      961 tattccgcga agtcggtggc gctcgggtag gccgggtcgc cgggccagcc cgcccaggtg
     1021 gcgttggcgc gggagaccga ggtgtggaac aggcgaacct ggctcaggtc gggggtcagc
     1081 cgctcgacct tgagcaggaa gccggcggtc ttgccgctga gggtgcctcc gacgatctgg
     1141 accttgacgt cggccagctc gccctcgcga aggtctgcca ccttggcgtc gccgtccttg
     1201 ccggtggcga acagcactcg gtcgtagtcg acggtgccgt tgtcggtgga gtcgtagagg
     1261 taggcgttga gcccgtactt gtcctgcccc gcgtcgatca cccgcagccg catctgcttg
     1321 gccgggctga acgaggtcgg cacgttggac cattcggtcg cgtcggtcgg agccgcggcc
     1381 gcgaaggccg gctgaccggc gaacccggcc gggtggtcga actgcaggcc aaagcttgcg
     1441 acgaactgcg cattgtcgct cggagagatg tagttggtcg ccacgccccg gccggagaag
     1501 aacgagagga agtcgatggt ggggccgtcg atctcgccat tgcgcccgcc ggcccactcc
     1561 acctgagcaa ccctcttgcc gccgcgttcg gcggcttggg cgatcgtctc agcctggagc
     1621 acgcccgggt caaaggccgc ggtgcggttg ccgaagggct ggccgctgat gtggaacgtg
     1681 ttgttggtcg agccgtgcac cgccggccac gcgccggtgg ccagcgagta ccagccggcg
     1741 ccggtgttgg gcggtgcctc ggtgagcagg ccggcgtcag atgcggaggt gcccttcttg
     1801 agcatctcgg ccatcgtcgg cagctcaccc tcggcggcgt actgcgccac caggtcctgg
     1861 cgcagcccat cggcggcgaa gaagacggcg gtgtcggtcg ggctggccgc cgccgtcgct
     1921 gtcgccgtgc gggccgcctg ggtcaccgag gcttgggccg gtgcgctgac ccccgggacc
     1981 accaacatcg atgtcgcagt gatcaagacg agtgcacgcc tgctgctcat gagcgacctc
     2041 cgcgcgtgac gcggtccccc ccggggccgt gatcgccgtc acgctaagcg cgggtcacaa
     2101 ggcctgccag cgcattggcg aaacatgggt agacgccggt gatggccgta atgccgcgtg
     2161 atggcgcgag ttgtgacgcg cttccggacc tctcagcctt cgagcttgta gccgagaccg
     2221 cggatggtca ggagcagctt gggggagccg ggctccgcct cgatcttgga gcgcagccgt
     2281 ttgacgtgga cgtcgagggt cttggtgtcg ccgacgtagt cggaccccca gacgcgcgtg
     2341 atcagctgat cgcgggtgag cgcccgcccg acgttgcgta gcaacagctc gagcaggtca
     2401 aactccttga ggggcaaggt gatctccgcg cccgagaccg tgacggtgtg ccggtcgacg
     2461 tccatccgga tcgggccggc ggccagcacg ccatcgtcgt acgacgcctc gtggtgctcg
     2521 tcgcgacggc gcagcacggc gcggatccgg gcgatcagct cccgtgtgga gaacggcttg
     2581 gtgacgtagt cgtcggcacc gagctcgagg ccgaccacct tgtcggtctc gctgtctcgt
     2641 gcggtgagca tgatgatcgg caccgtggac cgctgacgca gggcccggca gacctcggtg
     2701 ccgggcagcc cgggcagcat caggtcgagc agcacgaggt cgatctcgct tcggtcgaac
     2761 accgccaggg cgtccggtcc gctggcggcg tgcacgacct cgaacccctc cttctcgagc
     2821 atgtagccga gtgcgtcggc gaatgacgcc tcgtcctcca ccacgagcag ccgggtggtc
     2881 acgcggaggc ctcctgggtt ggtgcctgga tcgagcggtc ctcgagcatc ggtgcggcgg
     2941 gcaaggtgat ggtgaacgtc gagcccgatc gcacccggct gaacacgccg acccggccac
     3001 cgtggttcgc ggccacgtgc ttgacgatgg ccaggcccag gccggtgccg gcgccggagc
     3061 ctgatcgcgc cttgtcgacg cggtagaagc gttcgaagac ccgctcgagg tcctcctcgg
     3121 cgatcccgac gccggtgtcg gtcaccgcga tctccacggc cgccgccgcg tcgaggtcga
     3181 ccccttgccc acccggcttg cgccgtacgg tcgtcgagac cgtcatcccg tcgctgctgt
     3241 agcggatcgc gttgtcgagc aggttggcca cggccgtgac cagctgggcg cggttgccga
     3301 ggatggtcgc gtcgaggtcg ccgtcggcga ccagggacac cgaccgcatc tcggccatcg
     3361 cctgggcccg gtcggccgcc tcggccacga cctgcccgac acgcaccggc gtgggctcgg
     3421 gcagcggttc ggcgccctcc agccgtgaga ggtcgatcag ctcggagacc agccggccca
     3481 gccggaccga ctcgtgctcg atccggccgg caaaccgtcg tacggcgtcg gggtcgtcgc
     3541 tggccgcctg aagcgcctcc gcgagcagcg ccagtgcacc gaccggtgtc ttgagctcgt
     3601 ggctgacgtt gatgacgaag tcgcggcgca tctcggcgag ccggtgcgcc tcggtgatgt
     3661 cctccacgac gagcacgacc tggccggcgc ggtcagcggg ggcggccttg gccagcacgg
     3721 cgaccggatc gcgaggtagc catccgtgca gcagctggac ctgttgctcg tgacggtggc
     3781 cggtgcgccg ggtcttgtcg gcaagcgtgc gcagctcggg gatgctcagc gcctgaccac
     3841 gcaggagccc catggcccgc gcggcgtcgt tggccagcac cgcatcaccg gcggcgtcga
     3901 cgacgaccac gcccagccgg agctcgcgaa gcacggcggt gtcgaggacc gtggagtcgc
     3961 gacgccggac ggaccgcagc cgggtgcgcc aggtccagcc cagcaccagc gctgcgaccc
     4021 ccgcgacgag cacggcaagc agcgcctcca tgaccgacat gctaggtgtg ccggagaccc
     4081 agctcggcgc cctggcccgc cgtgagcgct agccatgcgc agctgttcac ctgatgttca
     4141 tgttggctcc gccttcggtt cacggacgat cactagcgtc cgccacatga agcgacgcac
     4201 cgcaagaaat gccgcagccc ttcccggggc gatcgtgctc gcactggccc ttgccgcctg
//
LOCUS       DATIBB010000074         8119 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_1615552, whole genome shotgun sequence.
ACCESSION   DATIBB010000074 DATIBB010000000
VERSION     DATIBB010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 8119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8119
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1615552"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..763
                     /locus_tag="VLC50_02605"
     CDS             <1..763
                     /locus_tag="VLC50_02605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF26388.1"
                     /translation="LVIPEPSKAEALRVGDRAARGFPRLYVDADVELSWRDVRVLAAA
                     LDEAGVLASAPERIIPMTEVSWAVRAYYAVWQELPRVRSGLFGRGVIAVSEAGHRRIA
                     TLPDCLSDDLVLSEAFAADERTLDTGASVVVHPPRTWADLIRRRIRAMTGNEQARSLG
                     LASYAARTTSADLRGLAVRRPALLPKIAVFLAATIAARRGARAARRRGDYTSWLRDES
                     SRRVEPPSGERGSLLNRVKGTPARDSASGPARAPR"
     gene            complement(699..1325)
                     /gene="pth"
                     /locus_tag="VLC50_02610"
     CDS             complement(699..1325)
                     /gene="pth"
                     /locus_tag="VLC50_02610"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013839602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HSF26389.1"
                     /translation="MTEEHDDTSWLVVGLGNPGPTYAGNRHNVGAMVLDELARRVGGR
                     FKAHKTRSVVCETRLGVRPGGAPGPRVVLAKPTSYMNESGGPVAGLVRFYGVPASRLV
                     VVHDELDIPFSTIRLKLGGGEGGHNGLRSISKSVGTKDYLRVRVGIGRPPGRMDPADY
                     VLRDFPSGDRAELALNVAAGADAVECLISEGLERAQMRYHAPECPSPG"
     gene            complement(1345..1941)
                     /locus_tag="VLC50_02615"
     CDS             complement(1345..1941)
                     /locus_tag="VLC50_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019873261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HSF26390.1"
                     /translation="MSEVKIAAEPRTEFGKGAARRIRRANKVPAVVYGHGGAPRHISL
                     PGHELMLALKSSNVLLQIDIEGTTELTLPKDVQRDPIKGFLEHVDLVVVRRGEKVTVE
                     VPVRVVGEAGPGSLVAVELQTLSIEAEATHIPAAIEVDVTGVEAGSQILAKDIALPQG
                     AALVTEAEMLVVNVGHAPTVAAEEAGEAVAEAAPAPEA"
     gene            complement(2161..3141)
                     /locus_tag="VLC50_02620"
     CDS             complement(2161..3141)
                     /locus_tag="VLC50_02620"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016472648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0044249 - cellular biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HSF26391.1"
                     /translation="MSYIKATGEKKLMLFSGRAHPELADAVAKELGVELVPTKVFDFA
                     NGEIYVRFLESARGSDAFVLQSHTARINNWIMEQLIMVDALKRASAKRITVVAPFYGY
                     ARQDKKHRGREPISARLMADLFKTAGADRIMSVDLHTAQIQGFFDGPVDHLHALPLLA
                     SYVGRRVDRAKLTIVSPDAGRVRVADQWTDRLGGAPLAIIHKRRDPNVANQVRVHEVV
                     GDVKGRTCVLVDDMIDTAGTITQAADALFEYGAAEVIVTATHAVLSGPAIDRLKNSRI
                     SEVVVTDTLPIPEDRRIDKLTVLSIAPLLGRAIREVFEDGSVTSLFDGAS"
     gene            complement(3146..4630)
                     /gene="glmU"
                     /locus_tag="VLC50_02625"
     CDS             complement(3146..4630)
                     /gene="glmU"
                     /locus_tag="VLC50_02625"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013839605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HSF26392.1"
                     /translation="MSQTRPAAVIVLAAGEGTRMRSATPKVLHTLGGRSLLGHAMAAA
                     RGLDPQHLVVVVRHQRDAVATHIGETDPAALVADQDDVAGTGRAVECALAVLPQLTGT
                     VVVTYGDVPLLSVDTLRELVATHVEGAHAVTVLTARVPDPTGYGRVVRAADGSVAAIV
                     EHKDATAEQRRIDEINSGIYAFDATVLAKLLPLVGTDNAQGEKYLTDVLALSREAGGR
                     VAALTIGDVWATEGVNDRVQLARMGAELNRRVLERWMRSGVTVIDPATTWVDVTVELA
                     RDVTLLPGVMLHGATTVAEGATVGPDCSLTDVEVGPSASVVRTHGSGAVIGEGATVGP
                     FSYLRPGTVLGRAGKIGAYVETKNARIGDGSKVPHLSYVGDAEIGEGTNIGAATVFVN
                     YDGVDKHHTVVGDHVRVGSDSMLIAPVTIGDGAYTAAGSVITNDVPPGAIAVARGRQR
                     NVEGWVARRRPGTAAARAASRALGQPDPAQTQTPGAGAEAPEEE"
     gene            complement(4751..4822)
                     /locus_tag="VLC50_02630"
     tRNA            complement(4751..4822)
                     /locus_tag="VLC50_02630"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4787..4789),aa:Gln,seq:ttg)
     gene            4984..5595
                     /locus_tag="VLC50_02635"
     CDS             4984..5595
                     /locus_tag="VLC50_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015577508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HSF26393.1"
                     /translation="MRMTGQQRREQLVDIGRALFADKGFDATSVEEIAAKAGVSKPVV
                     YEHFGGKEGLYAVVVDREMHALLDSITSALTGESHPRVLLEQAALALLGYIEASQDGF
                     RILVRDSPVGESTGGFASLISDATTHVEHILVEQFKASGFDPKTAPLYAQMLVGMVAL
                     TGQWWLDARKPKKAEVAAHLVNLAWNGLSRLETKPTMRAPRKH"
     gene            5614..6393
                     /locus_tag="VLC50_02640"
     CDS             5614..6393
                     /locus_tag="VLC50_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019045946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HSF26394.1"
                     /translation="MVLRVGTSGWQYADWRGPFYPRGCPQREWLRFYLSQFATVEVNA
                     TFYRLPTRETVARWAAEAPRDALFTVKASRFLTHIRRLREPAEPVARLLGVVEPLGPL
                     LGPVLLQLPPHMAVSASDLDATLGCFPAGVRVAVEPRDPSWFVDEVKDVLVRRDAALV
                     WADRLGRPTGPLWRTAGWGFLRLHQGSARPEPSYGRAALASWVDRVSRAWDVQSADVF
                     VYFNNDPHAAAPANARTFAGIAGRRGLAVSRVPANPVPVTS"
     gene            complement(6401..7171)
                     /locus_tag="VLC50_02645"
     CDS             complement(6401..7171)
                     /locus_tag="VLC50_02645"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018777546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030798 - trans-aconitate
                     2-methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HSF26395.1"
                     /translation="MQWDPEQYLRFGDHRARPFHDLLARVEADDPSYVVDLGCGPGPL
                     TELLARRWPHATVVGVDSSPEMIARAERLATDGLEFVRADLRDWTPEYPVDVLVSNAT
                     LQWVPDHLAQLPRLAAMLAPRGWLALQRPGNFDAPSHTELAAMRTSPRWRDRLAGLAE
                     RAAVHEPATYLATLAAAGLVVDAWETTYLQVLAGEDAVLEWMKGTGLRPTLELLTEPE
                     AAEFVAEYGERLRAAYPAQPFGTVLPFRRIFMVARRGV"
     gene            7275..7775
                     /locus_tag="VLC50_02650"
     CDS             7275..7775
                     /locus_tag="VLC50_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012851361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HSF26396.1"
                     /translation="MQDDVDRLIGAWRRERPDLDVTPLEVLSRVTRLARHLDLARRAA
                     FIEHDLEVWEFDVLAALRRAGSPYALSPGQLLTQTLVTSGTMTNRVDRLASKGLVERL
                     PDPRDRRGVQVRLTPKGQQRVDAAMADLIAREEELLAGLSTTARTRLASTLRTLLLPF
                     DAPSPS"
BASE COUNT         1148 a         2912 c         2900 g         1159 t
ORIGIN      
        1 cctcgtcatt cccgagcctt ccaaggccga ggcgctgcgg gtgggtgacc gggcggcgag
       61 aggtttcccg cggctctatg tcgatgccga cgtcgagctg tcctggcggg acgtgcgtgt
      121 gctggctgct gcgctcgacg aggcgggagt gctcgcgagc gcacccgagc gaatcatccc
      181 gatgactgag gtctcctggg cggtgcgggc ctattacgcg gtgtggcagg agcttccgag
      241 ggtcagaagc ggtctgttcg ggcgcggcgt gatcgccgtc tcggaggcgg ggcaccggcg
      301 gatcgccacg ctgccggact gcctctccga cgaccttgtc ctctccgagg cgttcgcggc
      361 cgacgaacgc accctcgaca ccggcgcgtc ggtggtcgtg cacccaccgc ggacctgggc
      421 cgacctgatc aggcggcgga tccgagcgat gaccggcaac gagcaggcgc gctcgctggg
      481 cctggcgtcg tacgccgctc gcaccacgag cgccgacctg cgcgggctcg cggtcagacg
      541 gccggcgctg ctgccaaaga tcgcggtgtt cctggccgcg acgatcgcgg cgcggcgcgg
      601 ggcccgggca gcccgccggc ggggcgacta cacgtcgtgg ctgcgcgacg agagcagccg
      661 ccgcgtcgag ccgccgtccg gcgagcgcgg ctcgctgctc aaccgggtga agggcactcc
      721 ggcgcgtgat agcgcatctg ggcccgctcg agcccctcgc tgatgaggca ctcgacggcg
      781 tcggcgccgg cggcgacgtt gagcgccagc tcggcacgat cgccggacgg gaagtcacgc
      841 agcacgtagt cggccgggtc catgcgcccg ggcgggcgcc cgatccccac tcgcactcgc
      901 aggtagtcct tggtgcccac cgacttgctg atcgatcgca ggccgttgtg gccgccctcg
      961 ccgccaccga gcttgagccg gatcgtcgag aacgggatgt cgagctcgtc gtggacgacg
     1021 accaaccggc tggccggcac gccgtagaac cgcacgagtc cggcgaccgg accgccggac
     1081 tcgttcatgt aggacgtcgg cttggccagc accacccgcg gcccgggcgc cccgccgggg
     1141 cgcaccccca gccgggtctc gcacaccacc gagcgcgtct tgtgcgcctt gaaccggccc
     1201 ccgacccggc gcgcgagctc gtcgagcacc atcgcgccga cgttgtgccg gttgccggcg
     1261 tacgtcggcc cggggttgcc caggccgacg accagccagc tcgtgtcgtc gtgctcctcg
     1321 gtcatgaccg accctgtggt cgggtcaggc ctcgggcgcc ggggccgcct cggccaccgc
     1381 ctcgccggcc tcctcggccg cgacggtcgg cgcgtggccg acgttgacga ccagcatctc
     1441 ggcctcggtc acgagcgccg cgccctgcgg gagcgcgatg tccttggcca ggatctggct
     1501 gcccgcctcg acccccgtca cgtcgacctc gatggcggcg gggatgtggg tggcctcggc
     1561 ctcgatcgac agcgtctgca gctcgaccgc gacgaggctg cccgggccgg cctcgccgac
     1621 cacccgcacc ggcacctcga cggtgacctt ctcgccgcga cgtacgacga cgaggtcgac
     1681 gtgctcgagg aagcccttga tcgggtcgcg ctggacgtcc ttgggcaggg tgagctcggt
     1741 cgtgccctcg atgtcgatct ggagcagcac gttggaggac ttcagcgcga gcatgagctc
     1801 gtggccaggc agcgagatgt gccggggggc gccaccgtgg ccgtagacga cggcaggcac
     1861 cttgttggcc cgacgaatcc gccgggcagc acccttgccg aactcggtgc gcggctcagc
     1921 ggcgatcttg acctcggaca cagcggtaac tcctcgacac gacgtgacgt tggcgcaggg
     1981 tctctgcgat gtcttggtgc ccagccggac gcacgtgcgt ctgccgcgtc gatgacggag
     2041 cgcgtacggc atccctcgcc gggcaacccg gtgagtctag cgggaccgag cgcgcacgcc
     2101 gaatcgcggg tcgacccgac gaggcccgcc cgtggggcgc ctgacttgag gccggtccgg
     2161 tcagctcgcc ccgtcgaaga ggctggtcac cgagccgtcc tcgaacacct cacggatcgc
     2221 ccgacccagc agcggcgcga tcgagagcac ggtgagcttg tcgatgcgcc ggtcctccgg
     2281 gatcggcagc gtgtcggtga cgaccacctc gctgatccgg gagttcttca gccggtcgat
     2341 cgccggcccg gagagcacgg cgtgggtcgc ggtgacgatc acttcggccg cgccgtactc
     2401 gaacagcgcg tcggcggcct gcgtgatcgt gccagccgtg tcgatcatgt cgtcgaccag
     2461 cacgcaggtg cggcccttga cgtcgccgac cacctcgtgg acccgaacct ggttggcgac
     2521 gttgggatcg cggcgcttgt ggatgatcgc gagcggagcg ccgccgagcc ggtcggtcca
     2581 ctggtcggcg acccggaccc gcccggcgtc cggagagacg atggtgagct tggcgcggtc
     2641 gacccggcgg ccgacgtagg aggccagcag cggcagcgcg tgcaggtggt cgaccgggcc
     2701 gtcgaagaag ccctggatct gggcggtgtg caggtcgacc gacatgatcc ggtcggcgcc
     2761 ggcggtcttg aacaggtcgg ccatcagccg cgccgagatc ggctcacggc cacggtgctt
     2821 cttgtcctgg cgggcgtagc cgtagaacgg ggcgaccacg gtgatccgct tggccgacgc
     2881 gcgcttgagc gcatcgacca tgatcagctg ctccatgatc cagttgttga tccgagcggt
     2941 gtggctctgc aggacgaacg cgtcggaccc gcgggccgac tcgaggaagc ggacgtagat
     3001 ctcgccgttg gcgaagtcga acaccttggt cggcaccagc tcgacgccga gctccttggc
     3061 caccgcgtcg gccagctccg ggtgggcccg accggagaag agcatgagct tcttctcgcc
     3121 ggtggccttg atgtaggaca cgccgctact cctcttctgg tgcctcggcc ccggcacccg
     3181 gggtctgtgt ctgcgcgggg tcgggctggc cgagcgcccg ggacgcggcc cgggcggcgg
     3241 ccgtgccggg tcggcgcctg gccacccagc cctcgacgtt gcgctgcctc ccgcgggcca
     3301 cggcgatcgc gcccggcggg acgtcattgg tgatcaccga gccggccgcc gtgtaggcgc
     3361 cgtcgccgat ggtgaccggg gcgatgagca tgctgtcgct gccgacccgc acgtggtcgc
     3421 ccaccacggt gtggtgcttg tcgacgccgt cgtagttgac gaagacggtc gccgcaccga
     3481 tgttggtgcc ctcaccgatc tcggcgtcgc cgacgtagga caggtgcggc accttggagc
     3541 cgtcgccgat gcgagcgttc ttggtctcga cgtaggcacc gatcttgccg gcgcgcccga
     3601 gcacggtgcc gggacgcagg taggagaacg ggcccacggt ggcgccctcg ccgatcaccg
     3661 cgccggaccc atgggtgcgt acgacggagg cgctcggccc gacctcgacg tcggtgagcg
     3721 agcaatcggg cccgacggtg gcaccctcgg cgaccgtcgt cgcgccgtgc agcatcacgc
     3781 cgggcagcag tgtgacgtcg cgagccagct cgacggtcac gtcgacccag gtggtcgccg
     3841 ggtcgatcac ggtgaccccg gaccgcatcc accgctcgag cacccgacgg ttgagctcgg
     3901 cacccatgcg ggccagctgc acccggtcgt tgaccccctc ggtcgcccac acgtcgccga
     3961 tggtcagcgc ggcgacccgc ccaccggcct cgcgcgagag ggccagcacg tcggtgaggt
     4021 acttctcccc ctgggcgttg tcggtgccca ccagcggcaa gagcttggcc agcacggtgg
     4081 cgtcgaatgc gtagatgccc gagttgatct cgtcgatccg ccgctgctcg gcggtggcgt
     4141 ccttgtgctc gacgatcgcg gccaccgacc cgtcggcggc gcggaccacg cggccgtagc
     4201 cggtggggtc gggcacccgg gcggtcagca cggtcaccgc gtgggcgccc tcgacgtggg
     4261 tggcgaccag ctcgcgcaac gtgtcgacgg aaagcagcgg cacgtcgccg tacgtcacga
     4321 cgaccgtgcc ggtcagctgg ggcagcaccg ccagcgcgca ctcgacggct cgcccggtgc
     4381 cggccacgtc gtcctggtcg gcgacgagcg cggccgggtc ggtctcgccg atgtgcgtgg
     4441 cgaccgcgtc gcgctgatgg cgtacgacga ccacgaggtg ctgcgggtcg agcccgcggg
     4501 cggctgccat cgcgtgaccc agcaggctgc ggccgccgag ggtgtgcagc accttcgggg
     4561 tggccgagcg catgcgggtg ccctcgcctg cggcgaggac gatgaccgcg gccgggcggg
     4621 tctggctcaa cgcagggagc tcctcggcat cgcgggcggg aggcagaacc gaggataccg
     4681 gccgttcacc gccgggtcac cgcggtgtca tcacaacggg cccacgccgc aatgcggaca
     4741 taagcggacg ctcccggaag aggattcgaa cccctactaa caggaccaaa acctgcggtg
     4801 ctgccgatta caccatccgg gaccgggatg cactccctgc cgcgcgggag cctacgccag
     4861 acacatccac ggacgagtct gacagtgatc gacaggcaca atgaggcagt gaccaccccc
     4921 acgacccgag ccaagaagtc cgcgccggcc ccgcgccgta cgacctcatc gtcaccgagc
     4981 cgcatgcgga tgaccggcca gcagcgtcgc gagcagctgg tcgacatcgg ccgggcgctg
     5041 ttcgccgaca agggcttcga cgcgaccagc gtcgaggaga tcgccgccaa ggcaggcgtg
     5101 tccaagccgg tggtctacga gcacttcggg ggcaaggagg ggctctacgc ggtcgtcgtc
     5161 gaccgggaga tgcacgccct gctcgacagc atcaccagcg cgctcaccgg cgagagccac
     5221 ccccgtgtgc tgctcgagca ggccgcgctg gcgctgctgg gctacatcga ggcgagccag
     5281 gacggcttcc ggatcctggt gcgcgactcg ccggtcggtg agtcgaccgg cgggttcgcc
     5341 agcctgatca gcgacgcgac cacccacgtc gagcacatcc tggtcgagca gttcaaggcc
     5401 agcgggttcg accccaagac cgccccgctc tacgcccaga tgctcgtcgg catggtcgcg
     5461 ctgaccgggc agtggtggct ggacgcccgc aagcccaaga aggccgaggt ggccgcgcac
     5521 ctggtcaacc tggcctggaa cggcctgtcc cgcctcgaga ccaagccgac gatgcgggcc
     5581 ccgcgcaagc actgacaagc gcaccctggg ctggtggtgc tgcgggtcgg gacgtcgggc
     5641 tggcagtacg ccgactggcg ggggcccttc tacccgcgtg ggtgcccgca gcgggagtgg
     5701 ctgcggttct acctgagcca gttcgccacg gtcgaggtca acgcgacgtt ctaccggctg
     5761 cccacccgcg agacggtggc gcgctgggca gccgaggcgc cgcgtgatgc gctgttcacc
     5821 gtcaaggcca gccgcttcct gacccacatc cgacggctgc gcgaaccggc cgagccggtg
     5881 gcccggctgc taggcgtggt cgaaccactc ggcccgctgc tcgggccggt gctgctccag
     5941 ctgcccccgc acatggccgt gtcggcgagc gatctcgatg ccacgctcgg ctgcttcccg
     6001 gccggagtgc gggtcgcggt ggagccgcgt gacccgtcgt ggttcgtcga cgaggtcaag
     6061 gacgtgctcg tgagacgaga cgcggcgctg gtctgggccg accggctcgg gcggcccacc
     6121 ggcccgttgt ggcgcaccgc cgggtggggc tttctccggc tgcaccaggg cagtgcccgg
     6181 ccggagccgt cgtacggccg tgcggccctg gcctcgtggg tcgaccgggt gagccgggcc
     6241 tgggacgtgc aatcggcaga cgtcttcgtc tacttcaaca acgacccgca cgccgccgcc
     6301 ccggccaacg cgcgcacgtt cgccggcatc gccggccgtc gcgggctcgc cgtcagccgg
     6361 gtccccgcca acccggtccc cgtcacgtca tgacccgccc tcacacgccc cgacgagcca
     6421 ccatgaagat ccgccggaag ggcagcacgg tgccgaacgg ctgcgccgga taggcggccc
     6481 gtagccgctc gccgtactcc gcgacgaact ccgcggcctc tggctcggtg agcagctcca
     6541 gcgtgggccg cagcccggtg cccttcatcc actcgagcac ggcgtcctcg ccagccagca
     6601 cctgcaggta ggtggtctcc caggcatcga cgacgagccc tgcggctgcc agcgtcgcga
     6661 ggtaggtcgc tggctcgtgg accgcggctc gttcggccag cccggcgagg cggtcgcgcc
     6721 agcgcggtga ggtgcgcatc gccgccagct cggtgtgcga gggggcgtcg aagttgccgg
     6781 gccgctgcag cgccagccac ccacggggcg cgagcatcgc cgccagccga gggagctggg
     6841 cgagatggtc gggcacccac tgcagggtgg cgttggagac gagcacgtcg accgggtact
     6901 ccggcgtcca gtcacgcagg tcggcacgga cgaactcgag accgtccgtg gccagccgct
     6961 cggcccgggc gatcatctcc ggcgaggagt ccaccccgac cacggtcgcg tgcggccacc
     7021 ggcgtgccag cagctcggtc agtggccccg gcccgcaccc gagatccacg acgtacgacg
     7081 gatcatcggc ctcgacgcgg gcgagcaggt cgtggaaggg ccgcgcccga tgatcgccaa
     7141 accgcaggta ttgctcgggg tcccactgca cgtcgcgcct cccggtgctg agctctcggc
     7201 tcgcaggatg gcggacaaga tatcttgatg tcaagaaact tcgcggcgag agtctcgtcg
     7261 ctagactgat caccatgcag gatgacgtcg accggctcat cggcgcatgg cggcgtgagc
     7321 gccccgacct cgacgtcacg ccgctcgagg tgctcagccg ggtcacccgg ctggcccgcc
     7381 acctcgacct ggcccgacgg gcggcgttca tcgagcacga cctcgaggtg tgggagtttg
     7441 acgtgctcgc cgcgctgcgc cgagccgggt cgccctacgc gctctccccc ggccagctgc
     7501 tcacccagac gctggtcacc tccggcacga tgaccaaccg ggtcgaccgg ctggccagca
     7561 agggactcgt cgagcggctg cccgacccgc gcgaccgccg cggggtgcag gtgcggctga
     7621 cccccaaggg ccagcaacgc gtcgacgccg ccatggccga cctcatcgcc cgtgaagaag
     7681 agctgctcgc cggcctgtcg accaccgcac gcactcggct ggcctcgacc ctgcgcacgc
     7741 tgctgctgcc cttcgacgcc cccagcccct cctagccctc cgccttgccg ccatgtgcgt
     7801 gatcaagcaa tccgcgctca cttctcttcg gtgatcaagc aatccgtgcc cgggggcagc
     7861 ggcagagcgg agactcaaag gacggagcaa cctggcactc gccgggtcgc tcggcggagc
     7921 gccgtacgcc cgcgttggtc cgtccgctgc gaccggccag cccgggcagc ggggcgacgt
     7981 acacggattg cttgatcatc aagacgattc gggcacggat tgcttgatca ccaacggggt
     8041 gcgggcacgg attgcttgat caccgccggg ggcgggggct actgggcggc ctcggccacc
     8101 tcgagccagc gctcctcga
//
LOCUS       DATIBB010000075         2441 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_1484243, whole genome shotgun sequence.
ACCESSION   DATIBB010000075 DATIBB010000000
VERSION     DATIBB010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2441)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2441)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2441
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1484243"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..196
                     /locus_tag="VLC50_02655"
     CDS             <1..196
                     /locus_tag="VLC50_02655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF26397.1"
                     /translation="GWTLTKINGTWTWISPTGHRYALDPVTVGTVITDITRHDAAFDL
                     TDEQPPDPTDLEPTGIADLD"
     gene            complement(224..910)
                     /locus_tag="VLC50_02660"
     CDS             complement(224..910)
                     /locus_tag="VLC50_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012889645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - intracellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HSF26398.1"
                     /translation="MLHEHRDVTGGWLRPAVFGAMDGLVSNFALIAGVVGGGASSQTV
                     ALAGLAGLAAGAFSMAAGEYTSVASQSELALAEIELERRELAAHPEVELEELAHMYAA
                     RGVDLDLAREFARQVSRDPERALEAHVWEELGVHPDDLPSPRLAAGSSFVSFAIGALL
                     PLLPYLFGASSIVPALAVALLGLFAAGSLVSLVTQRSWWFSGLRQLVLGGLAAALTYG
                     FGALVGAGLD"
     gene            complement(970..1842)
                     /gene="lgt"
                     /locus_tag="VLC50_02665"
     CDS             complement(970..1842)
                     /gene="lgt"
                     /locus_tag="VLC50_02665"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014141912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HSF26399.1"
                     /translation="MLLASIPSPPAGQIDLGPLPLRGYALCIILGVVVAVWLGERRWV
                     ARGGLPGEVSDIAIWAVPFGLVGGRLYHVVTTPELYFGEGGHPWQAFAIWRGGLGIWG
                     AIALGALGAWIACRRRGIPLPAMADALAPGIVLAQAIGRWGNYFNQELFGRPTALPWG
                     LQIDPAHRPVGYEQFATFHPTFLYESLWCVGVALLVIWADRRFTLGHGRAFALYVAAY
                     TVGRGWIEALRIDDAHHVLGLRLNDWTSLLVFAGAVTYIVVSARLRPGREAIDRGQPP
                     ASSPPSQPRDQVSP"
     gene            complement(1863..>2441)
                     /locus_tag="VLC50_02670"
     CDS             complement(1863..>2441)
                     /locus_tag="VLC50_02670"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HSF26400.1"
                     /translation="TVFAVLRSQVDADAPVPATATTQGGPLTVGTSGPVVDIFEDFQC
                     PFCQQFEATNGALLTDYAEAGKIQVNYHLASFIGQESVRAANAAACAADADAFPAFHA
                     TLYANQPPERTGGFTTDELIAHGKQVGIDSSAFKQCVRDSTYAEWVKASQHDFDTRGL
                     RGTPTVMIDGKVVDPTQYDAKTFKGLLDAAAS"
BASE COUNT          414 a          889 c          848 g          290 t
ORIGIN      
        1 cggctggacc ctcaccaaaa tcaacggcac ctggacctgg atctccccca ccggacaccg
       61 ctacgccctc gaccccgtca ccgtcggaac cgtcatcacc gacatcaccc gccacgacgc
      121 cgccttcgac ctcaccgacg aacaaccgcc ggaccccacc gacctcgaac ccaccgggat
      181 cgccgacctc gactagagcc tgccgcgaac ccgccgacct cgactagtcc agcccggccc
      241 cgacgagtgc cccgaagcca taggtgagcg ccgcggccag cccaccgaga accagctggc
      301 gcaggccgct gaaccaccag gagcgctgcg tgacaagcga gaccagcgac ccggcggcga
      361 acagtcccaa cagtgccacc gccaaggccg ggacgatgct gctcgccccg aacaggtagg
      421 gcagcagcgg cagcagcgcg ccgatcgcga aggacacgaa cgaggagcca gccgccagcc
      481 gcggagaggg caggtcgtcg ggatgcacgc cgagctcttc ccagacgtgc gcctcgaggg
      541 cacgctccgg gtcccgcgag acctgccggg cgaactcgcg ggccaggtcg aggtcgaccc
      601 cgcgggccgc atacatgtgc gcgagctctt cgagctcgac ctccgggtgc gcggccagct
      661 cgcggcgctc gagctcgatc tcggccagtg cgagctcaga ctgcgaggcg accgaggtgt
      721 actcgccggc ggccatcgag aacgccccgg cggccaaccc ggccagcccg gccagcgcca
      781 ccgtctgcga cgaggcaccc ccgccgacaa cgccggcgat caatgcgaag ttggagacca
      841 gcccgtccat cgcgccgaag acggcaggcc gtagccagcc gccggtgacg tcgcggtgct
      901 catggagcac ttcgacggtg gtgtcctccc cgccgggccc gccgacggcg ccggccgcgc
      961 tggaaccggt cacgggctca cctggtcacg cggctgcgaa ggcgggctcg atgccggggg
     1021 ttgccctcgg tcgatggcct cccgacctgg ccgcagccga gcggagacca cgatgtaggt
     1081 cacggcaccg gcaaagacca gcagcgacgt ccagtcgttg agccgcaggc cgagcacgtg
     1141 gtgggcgtcg tcgatgcgca gggcctcgat ccagccccgg cccacggtgt aggcggcgac
     1201 gtagagggcg aacgcgcggc catggccgag cgtgaaacga cggtcggccc agatcacgag
     1261 cagcgccacg ccgacgcacc agagcgactc gtagaggaac gtcgggtgga acgtcgcgaa
     1321 ctgctcgtag ccgaccggcc ggtgggccgg gtcgatctgc aggccccacg gcagcgcggt
     1381 cgggcggccg aacagctcct ggttgaagta gttgccccag cgaccgatcg cctgggccaa
     1441 gacgatgccc ggcgccagcg catcggccat cgccggcaac gggatccccc gccgccgaca
     1501 ggcgatccag gcgcccagcg cacccagtgc gatcgcgccc cagatgccga gtccgccgcg
     1561 ccagatggcg aaggcctgcc aggggtgacc accctcaccg aagtagagct ccggggtggt
     1621 gaccacgtgg tagagccgac cgccgacgag cccgaatggc accgcccaga tcgcgatgtc
     1681 ggagacctcg ccggggagtc cgccgcgggc cacccagcgt cgctccccca gccagacggc
     1741 gacgacgacg ccgaggatga tgcacagcgc gtagccccgc agtgggagcg gaccgaggtc
     1801 gatctgcccg gccggcggac tggggatgga tgccaggagc atgacggctc gacgctatcc
     1861 ggtcaggacg ccgcggcgtc gagtaggccc ttgaacgtct tcgcgtcgta ctgcgtggga
     1921 tccacgacct tcccgtcgat catcacggtc ggggtgccgc gcagcccgcg ggtgtcgaag
     1981 tcgtgctggg aggccttcac ccattcggcg tacgtcgagt cgcgcacgca ctgcttgaac
     2041 gcggacgagt cgatgccgac ctgcttcccg tgcgcgatca gctcgtcggt ggtgaagcct
     2101 cccgtgcgct ccggcggctg gttggcgtag agcgtcgcgt ggaaggccgg gaaggcgtcg
     2161 gcgtcggcgg cgcaggcggc cgcgttggcg gcgcgcacgg actcctgccc gatgaacgac
     2221 gccaagtggt agttgacctg gatcttgccc gcctcggcgt agtcggtgag cagggcgccg
     2281 ttggtggcct cgaactgctg gcagaacggg cactggaagt cctcgaagat gtcgacgacc
     2341 ggcccgctcg ttcccacggt gagagggccg ccctgggtcg tcgcggtcgc cggcaccggc
     2401 gcgtcggcgt cgacctgcga gcgcaacacc gcgaacacgg t
//
LOCUS       DATIBB010000076         9978 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_432857, whole genome shotgun sequence.
ACCESSION   DATIBB010000076 DATIBB010000000
VERSION     DATIBB010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 9978)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9978)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9978
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_432857"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..363
                     /locus_tag="VLC50_02675"
     CDS             <1..363
                     /locus_tag="VLC50_02675"
                     /inference="COORDINATES: protein motif:HMM:NF014660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliW"
                     /protein_id="HSF26401.1"
                     /translation="FPVGLPGFAGRRRFALVRVDESGLLFTLRSLEDPTLRLFVVPPA
                     PFFPDYAPEIDDADAALLELTDPADAQVLLVVTPGASPAEATANLLAPIVVNTRARVA
                     SQVILTDGQLSLRAPLAA"
     gene            complement(573..818)
                     /gene="csrA"
                     /locus_tag="VLC50_02680"
     CDS             complement(573..818)
                     /gene="csrA"
                     /locus_tag="VLC50_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011754246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HSF26402.1"
                     /translation="MLVLSRRTGESVVIGDDVTVTVLEVRGDVVRVGVDAPRHVKVHR
                     EEVYRELQEANRAAAAADDAAAANLAAALARPHDHEG"
     gene            complement(929..1417)
                     /locus_tag="VLC50_02685"
     CDS             complement(929..1417)
                     /locus_tag="VLC50_02685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF26403.1"
                     /translation="MPPTVVLPTPKDVRDTLADLLGRDVDVAPCEAVVPSNEAPCTLA
                     VFVDDAMTMSCVGVLDLPLSAWVGAAIGLVPAGGAADCVEVGELTPMVRDNLYEVLNV
                     LSATLNKPGASHQKLYAMHEPGSLPPTDVSSWAKVVVGRIDLAVTVAGYGSGRLGFVS
                     CV"
     gene            complement(1421..1786)
                     /locus_tag="VLC50_02690"
     CDS             complement(1421..1786)
                     /locus_tag="VLC50_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014694864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSF26404.1"
                     /translation="MKILVADDSRVMRQIVVRTLRQAGLGGHEIVEAADGQAAYDAVA
                     SEQPDLVLSDWNMPEMTGIELLQRLRASGQQVPFGFVTSEGSPAMREAAAREGALFLI
                     AKPFTAETFADVLTPVLGG"
     gene            complement(1783..2304)
                     /locus_tag="VLC50_02695"
     CDS             complement(1783..2304)
                     /locus_tag="VLC50_02695"
                     /inference="COORDINATES: protein motif:HMM:NF025070.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheX"
                     /protein_id="HSF26405.1"
                     /translation="MSLPVALVGEPELASIVDAAWTCALGVDDLPRQGPPVTGAGEWL
                     RAGVGIHGAWDGVVTLECTRTAAVGATSSMLALSPEAICDADVHDALGELANVVGGGV
                     KSLMPEHHRLTLPVVEPTTGVSTTSYDDLVARLDLTWRGEPVVVTVARTSPPAGSEPG
                     RPTARRTAGEPVS"
     gene            complement(2301..2684)
                     /locus_tag="VLC50_02700"
     CDS             complement(2301..2684)
                     /locus_tag="VLC50_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012085362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSF26406.1"
                     /translation="MLALVVDDSRAMRAILSRVLGGLGYETVQAGDGQAALDVLAAGP
                     LPDVALVDWNMPVMDGLAFVTAVRDNPAWRSITLMMVTTESEQSQIVRALAAGAHEYL
                     IKPFTPEAIAEKLELLGVLPLGAAS"
     gene            complement(2684..3556)
                     /locus_tag="VLC50_02705"
     CDS             complement(2684..3556)
                     /locus_tag="VLC50_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014378597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HSF26407.1"
                     /translation="MSLSCAAFDYVRQVVYADSAIVLEPGKEYLVESRLVPLARARGN
                     PDVTVFVAAAQRSGDREVRRLIVEALTTNETSWFRDAILWTGLREHVLPALARTRAVG
                     RRLSVWSAACSSGQETYSLAMMLADQLPGTDWSLDLLGTDLSEQMLARARSGRYSQLE
                     INRGLPAPMLVRHFVREGTDWLISPALRERVTFRQLNLATAFPPLPRFDLVLLRNVLI
                     YLDAETKRAILRRVRSVMVPDGYLVLGAAETTIGLDESWVREDIGGTAVYRPGTTASV
                     SELFADFSQTGVTH"
     gene            complement(3553..4665)
                     /locus_tag="VLC50_02710"
     CDS             complement(3553..4665)
                     /locus_tag="VLC50_02710"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011757030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HSF26408.1"
                     /translation="MGQISVLVVDDSVVVRRLVTTALEEDPAIRVIGTASNGRLALAK
                     IDQLAPDIVTLDVEMPELDGVATLRALRVDHPRLPVIMFSTVTERGARATLDALTAGA
                     TDYVTKPANMGSVAASLAAVREQLVPRIKALCPRDHAAVPTYPRGVPAAGAGLASART
                     APRARAGLALATRRPEILAIGASTGGPEALTTLLGALPADLPVPVVVVQHMPAVFTRL
                     FAERLNRSCAIEVGEAGDGQPLRPGVVYLAPGDHHLEVVGGPAGRRTRLTDAPPENYC
                     RPAVDVLFRSVAKACGAAALGLVLTGMGADGRRGAEQLREEGAAILVQDAATSVVWGM
                     PGAVAAAGLADAVLPLGEIAAAVLSRVRPSAVGVTR"
     gene            complement(4746..5954)
                     /locus_tag="VLC50_02715"
     CDS             complement(4746..5954)
                     /locus_tag="VLC50_02715"
                     /inference="COORDINATES: protein motif:HMM:NF020252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HSF26409.1"
                     /translation="MTAVHVGRHPIFDATRATYGYELLFRPVLERPSEPDQGEAATSQ
                     VIVDTFTEFGFDRLVGQGRAFVNVTTPFVVGGLLMPFGAPPETVLELPPTVRLDEPVV
                     AGIRARRAEGFAIAADDYDPDGSGRQLLDAELVDFVKIDVAAWSPEQLPEVVTSARRG
                     PATLVAKHVESVAALERCAELGIDLLQGCALDHPTATGAAGLAPTRAASLSLLAELTR
                     PDAEIDDVVALVECDVALSYRLVRAVNAASLGVRRKISSVRDALVMLGLETLRAWIVL
                     MSLAGEGESPSEPLRAALVRARHAELVAQAGATVRPSTAFMTGMLSTLDVLLGSDLPT
                     VVEALPLEAEVAAALLDGAGPLGQVLATVSAYERGRPVGGSDEIDAVRRSYLSATGWA
                     DSVCLLALAS"
     gene            complement(6032..6946)
                     /locus_tag="VLC50_02720"
     CDS             complement(6032..6946)
                     /locus_tag="VLC50_02720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF26410.1"
                     /translation="MYSLVSAPVLGFDLVRRSDGPAVAELLLRALTLRPYDVPALAAV
                     HRPGPARQAAWVDVVAAEAGAARMASIADQLHVDLAAAVTLLERVSIGTLPGLLRLVS
                     HEVLDWTWTSGPDGLAVRTPAAARAAEVVEDAIAALYVGALVEPVSHRVLLAPWQEFD
                     SAARSQPGRATTSEPVLVDLPGSADLPGPLAAALGRLARLDPADVGALRAAVDRDPAH
                     RTGWAMAAHRASWAVHLAGRTRAAAGAQLRAVTLVRAAGVSVADAAAGVWNLVGGALH
                     ALAVTDLLDADSHRRLVHPLREALGDLG"
     gene            complement(7009..8685)
                     /locus_tag="VLC50_02725"
     CDS             complement(7009..8685)
                     /locus_tag="VLC50_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013882532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HSF26411.1"
                     /translation="MFRRHTAAPAARAARPRRLVGERNPLERWMANRKVNTKIMLVMA
                     LLVAVAVSVGAVGLTALGKTNASTELLYRDSVKALIALSHVQQEQLRSQVLVANLVAA
                     PETEAETQQELIAEIARADADLARWEGNYANLGLSGQEDDWEAYRSAWTKFQRLRDAT
                     IDLAQRQVTVGFHAYDTVLSPEAQELIDASSDALDRVEAWEDQQAKSLMADAQSQFTL
                     ARSLMLAVLVVGLVLAIGLGLLAVALIVSPLRRVSRSLEAMAAGDLTVAVDVPGRDEL
                     GTMARALASAQQEMRSTVSALAGSAQTLTSASHELSQVSSRITAGAEETSAQSQVVAA
                     AAEQVSRNVQTVATGSEEMGASIREIAHSAHEAVQVAARAVGVAESTTATVGKLGESS
                     AEIGNVIKVITSIAAQTNLLALNATIEAARAGEAGKGFAVVAGEVKELAQETAKATED
                     IAARIQAIQVDTSGAVAAISEISQIIREINTFQLTIASAVEQQTATTNEMNRNVAEAA
                     TGSGEIAANISGVAIAASSTSESVATAERAAAELSRLSAELHRLVSGFTY"
     gene            complement(8748..9188)
                     /locus_tag="VLC50_02730"
     CDS             complement(8748..9188)
                     /locus_tag="VLC50_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020513051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HSF26412.1"
                     /translation="MATAQLATFELGGTLFGVPVERVQEVLRSQPRTPVPLAPPAVDG
                     LLNLRGQVVTALDLRTRLCLPDPAETGPGGESGEPMNVVVRAADEVVSLLVDVIGEVV
                     DVSDDAFEPPPATLVGPARELVHGAYKLDGRLLLALDIDRVVDI"
     gene            complement(9188..>9978)
                     /locus_tag="VLC50_02735"
     CDS             complement(9188..>9978)
                     /locus_tag="VLC50_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015625796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HSF26413.1"
                     /translation="LLPLVYLDESLGHPARARRTEESYIAVLQADGRRFGLVVDRVLN
                     TEEIVVKSLGRHLSDIGVYAGATILGDGAVALILDVASLARRALLAAQQVDAGLAGGT
                     DPSAGEGSAAVTELVLIVGVGTDRRVAVPLESVTRLEEFPAGIIEHLGAREVVQYRGE
                     IVPVVRLASLVGATSAPDGPTLRAIIHSQGERTVALVVDEIVDIAEREVAPEGDAAAA
                     VLRERVTELLDVRAAVRAFEIGTTRAEPAEALAIDAARLAGVSR"
BASE COUNT         1411 a         3686 c         3623 g         1258 t
ORIGIN      
        1 ttcccggtcg ggctgcccgg gttcgccggc cggcggcggt tcgcgctcgt gcgggtcgac
       61 gagtcggggc tgctgttcac gttgcgcagc ctcgaggacc cgacgctgcg gctgttcgtg
      121 gtgccgccgg cgccgttctt ccccgactac gcgcccgaga tcgacgacgc cgacgccgcg
      181 ctgctcgagt tgaccgaccc ggccgacgcg caggtgctgc tcgtcgtgac cccgggggcc
      241 tcgcccgccg aggcgacggc caacctgctg gccccgatcg tggtcaacac ccgcgcacgg
      301 gtggcctcgc aggtgatcct caccgacggc cagctctcgc tgcgcgcgcc cctcgccgcc
      361 tgacccctcg ccccctcctg ggcgtgatca agcaatccgt gcccgccccg cctcggtgat
      421 caagcaatcg gtgcacgcca gtgttcgggc gcaccacatc gtggaagatt gccgcctcag
      481 gacgtggacg gattgacacg atcaccggga atcgaggacg ccgagccgtc cgccgccaaa
      541 gtcgtgaagg ttaacccccg ctaaaggggc ggtcagcctt catgatcatg cgggcgggcg
      601 agcgccgcgg ccaggttggc cgccgccgcg tcgtcggccg ccgcggccgc ccggttggcc
      661 tcctgcagct cgcggtagac ctcttcgcgg tgcaccttca cgtggcgcgg ggcatcgact
      721 cccacccgga cgacgtcgcc gcgcacctcg agcaccgtca cggtgacgtc atcgccgatg
      781 acgacgcttt cgcccgtgcg gcggctgagg acgagcatgg cgaggtccct tccgtgggca
      841 gcgcggcgag ccggccgcgc gggcccagga taggggtcag ccgcagatcg aggtcagagg
      901 cagatgcggc gccacacgta tccgagggtc acacgcagga gacgaacccg agccggcccg
      961 agccatagcc agcgaccgtc accgcgaggt cgatccgccc gacgacgacc ttggcccagc
     1021 tcgacacgtc ggtcggcggc agcgagccgg gctcgtgcat cgcatagagc ttctggtgcg
     1081 aggcccccgg cttgttgagg gtcgccgaga gcacgttgag cacctcatag aggttgtcgc
     1141 gcaccatcgg cgtgagctcg ccgacctcga cgcagtcggc ggccccaccg gccggcacca
     1201 gcccgatcgc ggcgccgacc caggccgaca gcggcaggtc gagcaccccg acgcagctca
     1261 tcgtcatggc gtcgtcgacg aatacggcca gcgtgcaggg ggcctcgttg gacggcacga
     1321 ccgcctcgca cggagccacg tcaacgtcgc ggccgagcag gtcggcgagc gtgtcgcgca
     1381 cgtccttggg cgtgggcagc accacggtcg gcggcatcgg ttagcccccc agcaccgggg
     1441 tgagcacgtc ggcgaacgtc tcggcggtga acggcttggc gatgaggaac agcgccccct
     1501 cgcgggcggc cgcctcacgc atcgctggcg agccctcgct ggtgacgaag ccgaacggca
     1561 cctgctgccc cgaggcgcgc agccgctgca gcagctcgat ccccgtcatc tccggcatgt
     1621 tccagtcgga gagcacgagg tcgggctgct cggaggccac cgcgtcatag gcggcctgcc
     1681 cgtctgcggc ctcgacgatc tcgtggccgc cgagcccggc ctggcgcagc gtgcggacga
     1741 cgatctggcg catcacgcgg ctgtcgtcgg cgacaaggat cttcatgaca cgggttctcc
     1801 tgcggtacga cgtgcggtag ggcggccggg ctccgacccg gccggaggac tcgtgcgggc
     1861 gacggtgacc acgaccggct cgccgcgcca ggtgagatca agccgagcga caagatcgtc
     1921 gtacgacgtg gtgctgaccc ccgtcgtcgg ctcgacgacc ggcagcgtca gccggtggtg
     1981 ttcgggcatc aggctcttga ccccgccgcc gaccacgttg gccagctcgc ccagcgcgtc
     2041 gtgcacgtcg gcgtcgcaga tcgcctcggg cgagagggcg agcatcgatg acgtggcgcc
     2101 gacggccgcc gtacgggtgc actcgagggt gaccacgccg tcccaggcgc cgtggatgcc
     2161 gacgccggcg cgcagccact cccccgcacc ggtcaccggt gggccctgcc ggggcagatc
     2221 gtcgacgccg agcgcgcagg tccaggccgc gtcgacgatc gaggcgagct cgggctcgcc
     2281 gacgagcgcg accggcaggc tcacgaggcg gccccgaggg ggagcacccc gagcagctcg
     2341 agcttctcgg cgatcgcctc gggggtgaac ggcttgatga ggtactcgtg ggccccggcg
     2401 gccagggccc gcacgatctg cgactgctcg ctctcggtgg tgaccatcat cagcgtgatc
     2461 gagcgccagg ccgggttgtc gcgcaccgcg gtgacaaagg ccagcccgtc catcaccggc
     2521 atgttccagt cgaccagcgc cacgtcgggc aacggtccgg cggcgagcac gtcgagggcc
     2581 gcctggccgt cgccggcctg cacggtctcg tagcccaacc caccgagcac ccgcgagagg
     2641 atcgcgcgca tggcccggga gtcgtcgacg accagcgcca acatcagtgg gtcacccccg
     2701 tctgtgagaa gtctgcaaac agctcggaga cgctggccgt ggtcccgggg cgatagacgg
     2761 cggtgccgcc gatgtcctcg cgcacccacg actcgtccag cccgatcgtg gtctcggccg
     2821 ctccaagcac gagatagccg tcgggcacca tcaccgagcg cacccggcgc aggatcgccc
     2881 gtttggtctc ggcgtcgaga tagatgagca cgttgcgcag cagcaccagg tcgaagcgcg
     2941 gcagcggcgg gaacgccgtc gccaggttga gctggcggaa cgtcacgcgc tcgcgcaacg
     3001 ccgggctgat cagccagtcg gtgccctcgc gcacgaagtg gcgcaccagc atcggcgcgg
     3061 gcagcccacg gttgatctca agctggctgt agcgcccgga ccgggcccga gccagcatct
     3121 gctcggacag gtcggtcccg agcaggtcga gcgaccagtc ggtgcctggc agctggtcgg
     3181 ccagcatcat cgccaggctg taggtctcct gcccgctcga gcaggccgcc gaccacaccg
     3241 agagccgccg gccgaccgcc cgggtgcggg ccagcgcggg cagcacgtgc tcgcgcagcc
     3301 cggtccacag gatcgcgtcg cgaaaccatg acgtctcgtt ggtcgtcagc gcctcgacga
     3361 tgagccgacg gacctcccgg tcgccgctgc gctgggccgc ggcgacaaag acggtcacgt
     3421 cggggttgcc gcgcgcgcgg gccagcggga ccagccgcga ctcgacgagg tattccttgc
     3481 ccggctcgag cacaatcgcc gagtcggcgt agacgacctg acgcacgtag tcgaacgccg
     3541 cacaggacaa gctcatcgtg tgactcccac cgccgagggc cggactcgcg acagcaccgc
     3601 cgccgcgatc tcgccgaggg gcagcaccgc gtcggccagc ccggccgccg ccaccgcccc
     3661 gggcatcccc cagacgaccg aggtcgccgc gtcctgcacg aggatcgccg cgccctcctc
     3721 ccgcagctgc tcggccccgc gccgcccgtc cgcgcccatg ccggtcagga ccagcccgag
     3781 cgcggccgca ccacacgcct tcgccacgct gcggaagagc acgtcgacgg ccgggcggca
     3841 gtagttctct ggcggggcat cagtcaagcg cgtacggcgc ccagccggcc cgccgacgac
     3901 ctcgaggtga tggtcgccgg gcgcgaggta gaccacgccg gggcgcagcg gctgaccgtc
     3961 cccggcctcg ccgacctcga tcgcgcacga gcggttgagc cgctcggcga acagccgggt
     4021 gaacaccgcg ggcatgtgct ggaccacgac caccggcacc ggcaggtcgg ccggcagggc
     4081 acccagcagc gtggtgagcg cctcgggccc cccggtcgag gccccgatcg cgaggatctc
     4141 cggccgccgg gtcgcgagag ccaggccggc gcgggcccgc ggcgccgtac gcgccgacgc
     4201 cagaccggca ccggccgccg gcaccccgcg cgggtacgtc ggcaccgctg catgatcacg
     4261 agggcagagg gccttgatcc gcgggaccag ctgctcgcgg acggcggcca gcgaggccgc
     4321 cacgctgccc atgttggccg gcttggtcac gtagtcggtg gccccggcgg tcagcgcgtc
     4381 gagcgtggcc cgggccccgc gctcggtgac cgtgctgaac atgatcaccg gcaaccgcgg
     4441 gtggtcgacg cgcaacgcgc gcagcgtggc caccccgtcg agctcgggca tctccacgtc
     4501 aagagtcacg atgtcgggcg cgagctggtc gatcttggcc agcgccagcc gcccgttgga
     4561 cgcggtgccg atcacccgga tcgccgggtc ctcctcgagc gcggtggtca ccagccggcg
     4621 tacgacgacc gagtcgtcga cgaccagcac cgagatctgt cccaccactt cgccttctca
     4681 cctggtgata cgacgcgccg tcgtccccat cggcgggggg cggtcgcggc tgagccttct
     4741 gcgggtcagc tggccagcgc gagcaggcag accgagtcgg cccacccggt ggccgagaga
     4801 tagctgcgtc gtacggcgtc gatctcgtca ctgccgccga ccggccggcc ccgctcatag
     4861 gcggagaccg tcgccagcac ctggccgagc gggccggcgc cgtcgagcag ggcggccgcg
     4921 acctcggcct ccaggggcag cgcctcgacg accgtgggca ggtcactgcc gaggagcacg
     4981 tcgagggtgg agagcatccc ggtcatgaat gcggtcgacg gacgcaccgt ggcccccgcc
     5041 tgggcgacca gctcggcgtg gcgggcccgg accagcgcgg cccgcagcgg ttcgctgggc
     5101 gactcgccct cgccggccag cgacatcagc acgatccagg cgcgcagcgt ctcgaggccg
     5161 agcatgacca gcgcgtcgcg caccgaggag atcttccggc gcaccccgag gctggcggcg
     5221 ttgaccgccc ggacgaggcg atagctcagc gccacgtcgc actcgaccag cgcgacgacg
     5281 tcgtcgatct cggcgtcggg gcgggtcagc tcggcgagca gcgacagcga ggcggcccgg
     5341 gtcggggcca gcccggccgc gccggtggcg gtcgggtggt cgagggcgca gccctgcagc
     5401 aggtcgatgc cgagctcggc gcaccgttcg agcgcggcga ccgactcgac gtgcttggcc
     5461 accagggtgg ccggaccgcg ccgggccgag gtgaccacct cgggcagctg ctccggcgac
     5521 caggccgcga cgtcgatctt gacgaagtcg accagctcgg catcgagcag ctggcgaccg
     5581 gacccgtcgg ggtcgtagtc gtcggcggcg atcgcgaacc cttcggcccg ccgcgcccgg
     5641 atgcccgcga ccaccggctc gtccaggcgc accgtcgggg gcagctcgag cacggtctcc
     5701 ggcggggcac cgaacggcat caggagccca ccgacgacga acggcgtggt gacgttgacg
     5761 aacgcccggc cctgcccgac cagtcggtcg aagccgaact cggtgaacgt gtcgacgatg
     5821 acctggctgg tggccgcctc accctggtca ggctccgacg ggcgctccag cacgggtcgg
     5881 aacaacagct catagccgta ggtcgcccgg gtcgcgtcaa agatggggtg ccggccgaca
     5941 tggacggcgg tcatcgaagt cacctccgcg gccgcggcgt gcggcctcgt ccgcaccatc
     6001 ggcgggcgcg gcggcgactg aagcgttcgg ctcagccgag gtcgccgagc gcctcacgca
     6061 gcgggtgcac gagacggcgg tgggagtcgg catcgagcag gtcggtgacg gccagcgcgt
     6121 gcagggcgcc gccgacgagg ttccacaccc cggccgcggc gtcggcgacg ctgaccccgg
     6181 cggcgcgcac gagagtgacc gcgcgcagct gggcccccgc cgccgcccgg gtgcgcccgg
     6241 ctaggtgcac cgcccaggag gcccggtggg cggccatcgc ccagccggtg cggtgggccg
     6301 ggtcacggtc gacggcggcg cgcagcgcac ccacgtcggc cgggtcgagg cgggccagcc
     6361 ggcccagggc cgcggccagc ggccccggca ggtcggccga tcccggcagg tcgaccaaga
     6421 ccggctcgga cgtcgtcgcg cggcccggct gcgagcgcgc ggcactgtcg aactcctgcc
     6481 acggtgcgag cagcacccgg tggctgaccg gctcgaccag cgcgcccaca tagagggcgg
     6541 cgatcgcgtc ctcgaccacc tcggcggctc gggcggctgc gggcgtgcgc accgcgagcc
     6601 cgtcgggccc ggacgtccag gtccagtcga gcacctcgtg ggagaccagc cggagcaggc
     6661 cgggcagcgt gccgatgctc acccgctcga gcagggtcac ggccgccgcg agatcaacgt
     6721 gcagctggtc ggcgatggac gccattcggg cggctccagc ctcggcggcg accacgtcaa
     6781 cccaggcggc ctgccgcgcc ggtccgggcc ggtggacggc ggcgagggca gggacgtcgt
     6841 acggcctgag ggtgagggca cgcagcagca gctcggccac cgccgggccg tcgctgcggc
     6901 ggaccaggtc gaagccgagc acgggcgcgc tcacgagcga gtacatcggc gcccatgctc
     6961 ccgtcaccct cccccgcgat caagcagctt ccgcagaggg gacggggctc agtaggtgaa
     7021 gccgctgacc aggcggtgca gctcggcgct gagccgggac agctcggcgg cggcccgctc
     7081 ggcggtcgcg acgctctcgc tggtcgagct ggccgcgatc gccaccccgg agatgttggc
     7141 cgcgatctcg ccggagccgg tggccgcctc ggcgacgttg cggttcatct cgttggtggt
     7201 cgcggtctgc tgctcgaccg ccgaggcgat cgtgagctgg aacgtgttga tctcgcggat
     7261 gatctgcgag atctccgaga tcgcggccac cgcgccgctg gtgtcgacct ggatcgcctg
     7321 gatgcgcgcg gcgatgtcct cggtcgcctt ggcggtctcc tgggccagct ccttgacctc
     7381 gcccgccacc acggcgaagc ccttgcccgc ctccccggcg cgcgcggcct cgatcgtcgc
     7441 gttgagcgcc agcaagttgg tctgcgccgc gatcgaggtg atcaccttga tgacgttgcc
     7501 gatctcggcg gaggactcgc cgagcttgcc gaccgtggcc gtggtcgact cggccacgcc
     7561 gaccgcacgg gcggccacct gcaccgcctc gtgggcgctg tgggcgatct cccggatcga
     7621 ggcgcccatc tcctccgagc cggtcgccac cgtctgcacg ttgcgcgaga cctgctcggc
     7681 ggccgcagcg accacctgcg actgcgccga ggtctcctcc gccccggcag tgatccgcga
     7741 cgagacctgc gagagctcgt ggctggccga ggtgagggtc tgcgccgagc cggccagcgc
     7801 cgagacggtc gagcgcatct cctgctgcgc gctggccaga gcccgggcca tcgtccccag
     7861 ctcgtcgcgg ccgggcacgt cgacggccac cgtgaggtcg ccggcggcca tcgcctcgag
     7921 cgagcgcgac acccggcgca gcggagagac gatcagcgct acggcgagca gcccgaggcc
     7981 gatcgccagc accaggccga ccaccagcac ggcgagcatc agcgagcggg caagggtgaa
     8041 ctgggactgg gcgtcggcca tcaggctctt ggcctgctgg tcctcccagg cctcgacccg
     8101 gtccagcgcg tcggacgagg cgtcgatcag ctcctgcgcc tccgggctga gcacggtgtc
     8161 gtaggcgtgg aacccgacgg tgacctgccg ctgggccagg tcgatggtgg cgtcgcgcag
     8221 ccgctggaac ttcgtccacg ccgaccggta ggcctcccag tcgtcctcct gcccgctcag
     8281 cccgaggttg gcgtagttgc cctcccagcg cgcgaggtcg gcgtcggccc gggcgatctc
     8341 ggcgatgagc tcctgctggg tctcggcctc ggtttcgggc gcggcgacga ggttggcgac
     8401 gaggacctgg gagcgcagct gctcctgctg cacgtggctg agcgcgataa gcgccttgac
     8461 gctgtcgcgg tagagcagct cggtcgacgc gttggtcttg cccagcgccg tcagcccgac
     8521 cgcgccgacc gacaccgcga ccgcgacgag cagcgccatc accagcatga tcttggtgtt
     8581 gaccttgcgg ttggccatcc agcgctcgag cgggttgcgt tccccgacga gacgccgggg
     8641 ccgggcagca cgagcggccg gggcggcggt gtgacggcgg aacatggcga tcccctcagc
     8701 agagacgtac ggcgagcgcg ggcgtggacg tgagcggcgg catggcctca gatgtcgacg
     8761 acccggtcga tgtccagggc cagcagcagc cggccgtcga gcttgtaggc gccgtggacg
     8821 agctcgcggg ccgggccgac cagggtggcc ggcggcggct cgaacgcgtc gtcgctgacg
     8881 tcgaccacct cgccgatcac gtcgacgagc aggctgacca cctcgtcggc ggcgcgcacg
     8941 accacgttca tcggctcgcc cgactccccg ccgggcccgg tctcggccgg gtcaggcagg
     9001 cagaggcggg tgcgcaggtc gagtgcggtg acgacctgcc cgcgcaggtt gagcagcccg
     9061 tcgaccgccg gcggggccag cggcaccggg gtgcgcggct gcgagcgcag cacctcctgc
     9121 acccgctcga cgggtacgcc gaacagcgtg ccgcccagct caaacgtcgc gagctgcgcg
     9181 gtggccatca gcggctcacc cccgccaacc gggccgcgtc gatcgccagc gcttcggccg
     9241 gctcggcccg cgtcgtaccg atctcgaacg cccgcaccgc ggcgcgcaca tcgagcagct
     9301 cggtgacccg ctcgcgcagc accgcggcgg ccgcgtcacc ctccggggcg acctcccgct
     9361 cggcgatgtc gacgatctcg tcgacgacga gcgcgacggt ccgctcgccc tgcgagtgga
     9421 tgatcgcgcg cagcgtggga ccgtccggtg cgctggtcgc cccgacgagg ctggcgagcc
     9481 ggacgaccgg cacgatctcg ccgcggtact gcaccacctc ccgggccccg aggtgctcga
     9541 tgatcccggc ggggaactcc tcgagccggg tgaccgactc gagcggtacg gcgacccggc
     9601 ggtcggtccc gaccccgacg atgagcacca gctcggtgac ggccgcggag ccctcgccgg
     9661 ccgacgggtc cgtgcccccg gcgagaccgg cgtcgacctg ctgggccgca agcagcgcgc
     9721 gccgggccaa cgaggcgacg tcgaggatca gcgcgaccgc gccgtcgccg aggatcgtcg
     9781 cgccggcata gacgccgatg tctgacaggt gccggcccag cgacttgacg acgatctcct
     9841 cggtgttgag gacccgatcg accaccagcc cgaaccggcg cccgtcggcc tgcagcaccg
     9901 cgatgtagga ctcctcggtc cggcgcgcgc gggccgggtg cccgagcgac tcgtcgaggt
     9961 agaccagcgg caacagct
//
LOCUS       DATIBB010000077         3189 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_169857, whole genome shotgun sequence.
ACCESSION   DATIBB010000077 DATIBB010000000
VERSION     DATIBB010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3189
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_169857"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1418
                     /locus_tag="VLC50_02740"
     CDS             <1..1418
                     /locus_tag="VLC50_02740"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019877781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HSF26414.1"
                     /translation="AVVHRGQVVARYAKHHLPNYGVFDEFRYFVPGDRLVVVRVHGID
                     VAVAICEDLWQEGGPVASTHDASAGLLVVINASPYEQTKGDVRLDLVGRRAAAAGATL
                     AYVNMVGGQDELVFDGDSLVASCDGSVLARAPQFEPGCLVVDLDLPEASADATELVPG
                     VDRVVISEAPCPPYEPILAGQADRLSGPAEVYTALVTGLRDYVRKNRFTTVVLGLSGG
                     IDSALTAAIACDAIGADNVHGVSLPSGYSSEHSRTDAAELAERTGLHYRSVAIAPMVE
                     AFLAELKLTGLAEENLQARVRGMTLMGLSNQEGHLVLATGNKSELAVGYSTLYGDAVG
                     GFAPLKDVPKSLVWELSRWRNAEATRRGETPPIPESSITKPPSAELRPGQLDTDSLPD
                     YAELDAVLDAYIEADEGAAQIVAAGHDPALVERVLRLTDAAEYKRRQYPPGPKISLKA
                     FGRDRRLPITNRWRESPSERG"
     gene            1519..2391
                     /gene="panB"
                     /locus_tag="VLC50_02745"
     CDS             1519..2391
                     /gene="panB"
                     /locus_tag="VLC50_02745"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009073144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HSF26415.1"
                     /translation="MPAGSAPGGSSDSTSHPTTLYGGITYRRVTIRDLQQAKDRGERW
                     AMLTSYDQMSAQVFDEAGIPVLLVGDSAANNALGYADTMQVTVDELLPLVRAVTRSTK
                     RALVTADMPFGSYQLSPQQALGTAIRFMKEGGAQAVKLEGGARVVPHVRAIVEAGIPV
                     MGHVGFTPQSVHALGGYRVQGRGDDAERLIADCLALEEAGAFSIVLELVPSEVAKRAT
                     AELTIPTIGIGAGVDCDAQVLVWTDMAGLTGGKVPRFVKRYADLRGILGAAAKAYMDD
                     VVAGTYPDTEHSYT"
     gene            2465..3166
                     /locus_tag="VLC50_02750"
     CDS             2465..3166
                     /locus_tag="VLC50_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017591004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF26416.1"
                     /translation="MDASHVAMLRELLAGSGWIDRTREFARSLRRSARPGGLLLVGTP
                     SEEPWHLAAHLSDESRLAGRPELEPTLVRWDPPAGAPAHLAVGLSRLEAAARGETLFV
                     VAPDQAPASLLERISDARGVGATILALDAGDPELEGLAHDTLTVPGIGPTVRTGSGLH
                     VPASYAETARRPAEAVEDVPAEALVSMATVQHLVSTAAGEPDRPGRRGLRERLSRFLD
                     AVSGPSPDGGGPPHW"
BASE COUNT          437 a         1233 c         1106 g          413 t
ORIGIN      
        1 cggcggtggt ccaccgcggc caggtggtgg cccgctacgc caagcaccac ctgcccaact
       61 acggcgtttt cgacgagttc cgctacttcg tgcccggcga ccggctggtc gtcgtgcggg
      121 tgcacgggat cgacgtcgcg gtggccatct gcgaagacct gtggcaggag ggaggtccgg
      181 tcgcctccac ccacgacgcc agcgccggcc tgctcgtcgt gatcaacgcc tcgccctacg
      241 agcagaccaa gggcgacgtg cgcctcgacc tggtcgggcg ccgggccgcg gccgccggcg
      301 cgacgctggc ctacgtcaac atggtcggcg gccaggacga gctggtcttc gacggcgact
      361 cgctggtcgc ctcctgcgac ggcagcgtgc tcgcccgggc cccgcagttc gagccgggct
      421 gcctcgtcgt cgacctcgac ctgccggagg ccagcgccga cgccaccgag ctggtccccg
      481 gcgtcgaccg cgtggtgatc agcgaggcgc cgtgcccgcc gtacgaaccg atccttgccg
      541 gacaggccga ccggctgagc gggcccgccg aggtctacac cgcgctggtc accgggctgc
      601 gggactacgt gcgcaagaac cgcttcacca ccgtggtgct cggactctcc ggcggcatcg
      661 actcggcact gaccgcggcc atcgcctgcg acgcgatcgg cgccgacaac gtccacggcg
      721 tctcgctgcc gagcggctac tcctccgagc actcccgcac cgacgccgcc gagctggccg
      781 agcgcaccgg gctgcactac cgcagcgtgg cgatcgcgcc gatggtcgag gcgttcctcg
      841 ccgagctcaa gctgaccggg ctggccgagg agaacctgca ggcccgggtg cgcggcatga
      901 cgctgatggg gctgtccaac caggagggcc acctggtgct cgcgaccggc aacaagagcg
      961 agctcgcggt cggctactcc accctctacg gcgacgcggt gggcgggttc gccccgctca
     1021 aggacgtgcc caagtcgctc gtctgggagc tgtcccggtg gcgcaacgcc gaggcgaccc
     1081 ggcggggcga gaccccgccg atccccgaga gcagcatcac caagccgccg tcggccgagc
     1141 tgcgtccggg ccagctcgac accgactcgc tgcccgacta cgccgagctc gacgccgtgc
     1201 tcgacgccta catcgaggcc gacgagggcg ccgcgcagat cgtcgcggcc ggtcacgacc
     1261 cggcactggt ggaacgggtg ctgcgcctga ccgacgccgc cgagtacaag cgccggcagt
     1321 acccgcccgg ccccaagatc tccctcaagg cgttcggccg cgaccggcgg ctgccgatca
     1381 ccaaccgctg gcgcgagtca cccagcgagc gcgggtgagc actgtcggcg cccggctggc
     1441 tcgtggcacg atgagcacgt ccggggacgc caccacggcg cctcgagatc ggagaccacg
     1501 atgacccacg actccagcat gcctgcaggg tctgcacccg ggggctcgag cgactcgacg
     1561 agccacccca ccaccctgta cggcggcatc acctaccggc gcgtgacgat ccgcgacctg
     1621 cagcaggcca aggaccgcgg cgaacgctgg gccatgctca cctcctacga ccagatgagc
     1681 gcgcaggtct tcgacgaggc cggcatcccg gtgctgctcg tcggcgactc cgcggccaac
     1741 aacgcgctcg gctacgccga cacgatgcag gtcacggtcg acgagctgct gccgctggtg
     1801 cgtgcggtga cccgctcgac caagcgcgcg ctggtcaccg ccgacatgcc gttcggctcc
     1861 taccagctct ccccgcagca ggcgctgggc accgcgatcc ggttcatgaa ggagggcggg
     1921 gcccaagccg tcaagctcga gggcggcgcg cgcgtcgtac cccatgtgcg cgcgatcgtg
     1981 gaggccggca tcccggtgat gggccacgtc ggcttcaccc cgcagagcgt gcacgccctg
     2041 ggcggctacc gggtgcaggg ccgcggtgac gacgccgagc ggctgatcgc cgactgcctg
     2101 gcgctggagg aggccggcgc gttctcgatc gtgctcgagc tggtccccag cgaggttgcc
     2161 aagcgggcca ccgccgagct gaccatcccg accatcggca tcggcgccgg cgtggactgc
     2221 gacgcccagg tgctggtctg gacggacatg gccgggctca ccggcggcaa ggtgccgcgc
     2281 ttcgtcaagc gctacgccga cctgcgcggc atcctgggcg ccgcggccaa ggcctacatg
     2341 gacgacgtcg tcgccggcac ctaccccgac accgagcact cctacacctg agcctcggcc
     2401 cgacgcggcg ccagcccgtg tcggtcccgg gaccgcgccc ttccctgcgg cagcatgagc
     2461 cgacgtggac gcctcccacg tcgcgatgct gcgtgagctg ctggccggct ccggctggat
     2521 cgaccgcacg cgtgagttcg cccggtcgct gcgccgctcc gctcgccccg gcgggctgct
     2581 gctagtcggc actcccagcg aggagccctg gcacctggcc gcccacctct ccgacgagtc
     2641 ccggctggcc gggcgccccg agctcgagcc gaccctggtg cggtgggacc caccggccgg
     2701 cgctccggct cacctggccg tcggcctcag ccggctcgag gcggccgcgc gcggcgagac
     2761 gctgttcgtc gtcgcccccg accaggcccc ggcctcgttg ctcgagcgca tcagcgacgc
     2821 ccgcggcgtc ggagccacga tcctcgcgct cgacgccggt gatcccgagc ttgaggggtt
     2881 ggcccacgac accctgacgg tgccgggcat cggtcccacg gtccggaccg gcagcggtct
     2941 gcacgtgccc gcgtcgtacg ccgagacggc gcggcgaccc gccgaggcgg tcgaagacgt
     3001 ccctgccgag gcgctggtct cgatggcgac cgtgcagcac ctggtcagca ccgccgccgg
     3061 cgagcccgac cgcccaggcc ggcgcgggct gcgcgagcgg ctctcgcgct tcctcgacgc
     3121 cgtcagcggc cccagccccg acggcggcgg cccacctcac tggtagtcag tcgtcgtcgt
     3181 cctcgtcgc
//
LOCUS       DATIBB010000078         9754 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_828674, whole genome shotgun sequence.
ACCESSION   DATIBB010000078 DATIBB010000000
VERSION     DATIBB010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 9754)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9754)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9754
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_828674"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(72..644)
                     /locus_tag="VLC50_02755"
     CDS             complement(72..644)
                     /locus_tag="VLC50_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013884173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HhH-GPD-type base excision DNA repair protein"
                     /protein_id="HSF26417.1"
                     /translation="MSLQLTGVPEADAVLEQDPFALVSAMMLDQQYPMEHAFRGPWKV
                     LERFGTLEPAAIAGADPDAFAALCATPPAVHRYARSMAARLQELARVVVDDYDGDASR
                     IWTEAATGADLLRRVQALPGFGKQKAAIFVALLAKQRGVRPDGWEKAAGDYALEGYRS
                     VADVVDAESLQRVRDYKKAKKAEAATRRTS"
     gene            complement(616..1407)
                     /locus_tag="VLC50_02760"
     CDS             complement(616..1407)
                     /locus_tag="VLC50_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009084737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HSF26418.1"
                     /translation="MGATVRRPARPWTPAVRVLLDRLRAAGVSQAPAWRGVDDQGRDV
                     FDFLPGDVGSYPLSAAIRSDAALITAARLLRSLHDASVPLTRESSLPWQLVPLEPVEV
                     ICHGDFAPYNCVFRGQEAVGVIDFDFARPGPRRWDLAYAIYRFAPLTAPGNADGFGDP
                     PAQARRARLFLDSYGCTARERRDALETIGPRLRWLVDHMRDAAAGGNRRFAAHIEAGH
                     ADTYLRDIAYVDAHFARGLELPGGQIAEAKDEQGWSHVVAADRGA"
     gene            complement(1485..2078)
                     /locus_tag="VLC50_02765"
     CDS             complement(1485..2078)
                     /locus_tag="VLC50_02765"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011979363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HSF26419.1"
                     /translation="MTDAARDPMSVPDDLPRPHDDGAAAHLPGRAMPALTLPATTGER
                     LPVGRPPIGFETLVLYAYPRTGRPGEPPLTPDWDLIPGARGCTPESCSFRDHARELGA
                     LGAAVLGVSTQDTDYQQEAAARLHLPFPLLSDADLELARALDLPTMTIAGQVLLKRLT
                     LIVRDGVIARAFYPVFPPHEHADHVLAWLRDHPGRAA"
     gene            complement(2155..2227)
                     /locus_tag="VLC50_02770"
     tRNA            complement(2155..2227)
                     /locus_tag="VLC50_02770"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2192..2194),aa:Thr,seq:cgt)
     gene            complement(2321..3898)
                     /locus_tag="VLC50_02775"
     CDS             complement(2321..3898)
                     /locus_tag="VLC50_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020577083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HSF26420.1"
                     /translation="MTRPTRLAAATLALGVLLTGCSGDPTGVPSPSTHTGPTGAETRP
                     APPASAPADDPATDPALASYYQQTLRWASCESASGTAGFECATLTVPVDYADPDGPTL
                     GLAVNRLPASGPRLGSLVLNPGGPGGSGVEYAGYATAVVSSPVRERYDVVGFDPRGVA
                     TSDPVRCLDDRETDVFLAADASPDDAAEVARLVELSQQLGQRCQARNPEVLAHLGTED
                     VARDLDVLRAALGDDDLHYLGKSYGTFIGATYAEMFPWRVGRLVLDGALDPRSTSAEI
                     NLGQAKGFEVAFSAFLADCLGKRTCPLTGDEADARAQLADFLAAVDRRPLPAGDRQLT
                     QALAVLGVVFAMYDEVFWPQLRSALKAGLSGDGRPLLGLADAYADRGTDGRYGSNQND
                     VIYAVNCLDRPEPDGPEQLAAAQPGFEKASPLFGAYIAWSSLPCHYWPVKAESAPGAL
                     RAAGAAPILVVGTTRDPATPYVWAQGLAEQLDSGVLLTYEGDGHTAYRRGSDCVDSAV
                     DAYLLKGETPAEGTRCD"
     gene            complement(3895..5061)
                     /locus_tag="VLC50_02780"
     CDS             complement(3895..5061)
                     /locus_tag="VLC50_02780"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017947788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HSF26421.1"
                     /translation="MSVWDDLVGQERVVETLRAAAADAAGVLAGQPASAMTHAWLITG
                     PPGSGRSTAARAFTAALQCLDGGCGECTACRTVRSGSHADLEVVSTEALTISVKDARE
                     LVLRASRHPAGGRWQVVIVEDADRLTEQAANALLKAVEEPAARTVWLLCAPSPEDVLP
                     TIRSRCRHVLLRTPPVAAVAAFLTARDGVDPAMAAFAARAVGGPIGRARRLAQDESAR
                     LRRAEVLRVPTIVTGVAQAVAAAAALVEAATEEAAATTAELDVREAEALGRALGEGTT
                     GRAMSGSARAALKDLEKAQKQRATRVKRDALDRALTDLVTFYRDVLAVQVGADDVGLT
                     NAELRPQIDRVAATSTPEVTLGRMEAILAAREAVEANVAPLLAVEAMALRLRQP"
     gene            complement(5058..5720)
                     /gene="tmk"
                     /locus_tag="VLC50_02785"
     CDS             complement(5058..5720)
                     /gene="tmk"
                     /locus_tag="VLC50_02785"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HSF26422.1"
                     /translation="MPEPTPALADPRGLLIAFEGGEGAGKSTQAKRLVGWLEAAGRET
                     VLTFEPGDTELGQALRAMLLHLDGPDPSARAEALLYAADRAEHVTRVIRPALARGAVV
                     VTDRFVDSSIAYQGAGRDLGADAVEALSTFATDGLRPDLTVLLDVAPQVGLHRFERPP
                     DRLEAEPLAFHARVRDAFARLAAAEPARYLVVDASAEPDRVWEQVRDRVTELLAAREG
                     SA"
     gene            complement(5756..8419)
                     /gene="topA"
                     /locus_tag="VLC50_02790"
     CDS             complement(5756..8419)
                     /gene="topA"
                     /locus_tag="VLC50_02790"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019433970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HSF26423.1"
                     /translation="MPAKSQAGNRRLVIVESPAKAKTIKGYLGAGYDVEASIGHIRDL
                     PTPSELPAAEKKGRFGKFAVDVDNGFEPFYVVDADKKKKVAELRKLLAEADELYLATD
                     EDREGEAIAWHLREVLKPRVPVRRMVFHEITRDAIREAVEHTRELDQSLVDAQETRRI
                     LDRLYGYEVSPVLWRKVRAGLSAGRVQSVATRLVVERERERIAFRVASYWDVEGRFVA
                     AAGEQTPFIARLVAVDGRRVATGRDFGRDGSVLAGRDVAVLDETGARSLADALGGREF
                     AVRSVEDKPYTRKPAAPFMTSTLQQEASRKLRLSSQVAMRVAQRLYENGYITYMRTDS
                     TTLSESALQAARSQARELFGADHVPDAPRRYERKVKNAQEAHEAIRPAGDVFRTPGQV
                     SGELRGDEFALYELIWKRTIASQMRDAVGTTATVRLGAVAADGRDAEFSASGTVITFR
                     GFLAAYEEGVDDDTERADRDGSDLRERRLPALRTGDALTERGLEADGHETTPPARYTE
                     ATLVKALEERGIGRPSTYASIMGTILDRGYVFKKGTALVPTWLAFSVTGLLERHFGRL
                     VDYDFTATMEEDLDRIAGGNEDRVAWLSRFYFGNGGTDQLEGLKRLVEGLGDIDAREV
                     NTIPVGNEIVLRVGRYGPYVERGEERASVPDDLAPDELTVERAEELLAAPSGDRELGV
                     DPATGREIVAKAGRFGPYVTEVLPEDAPKSAKPRTASLFKTMTLETVTLDDALRLLTL
                     PRVVGADPESGEEITAQNGRYGPYLKKGADSRSLDSEDQLFTITLEEALAIYAQPKQR
                     GRRAAAAPLKELGADPVTGKPVVVKDGRFGPYVTDGETNATLRKGDDPTTVSIERASE
                     LLAEKRAKGPAKKPARKTAARRTTKKAAPKR"
     gene            complement(8563..>9754)
                     /locus_tag="VLC50_02795"
     CDS             complement(8563..>9754)
                     /locus_tag="VLC50_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012782916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HSF26424.1"
                     /translation="TGYYTGTDKRPVQDIGKTSLTGAATVILSGISLGLESAVYTAVV
                     IGAAVYGAYLLGGGVVIISLFAVALAGTGLLTTVGVIVAMDTFGPVSDNAQGIAEMSG
                     DVHGEGAQILTELDAVGNTTKAITKGIAIATAVLAATALFGSYTDAVRTASDGVGEVV
                     TDFLNNSLVFSPNTLVGIILGAAVVFLFSGLAINAVARAAGAVVFEVRQQFADHPGIM
                     DGTERPEYGRVVDICTRDSLRELATPGLLAVLAPIAVGFGLGVGPLAGFLAGAIATGT
                     LMAIFLANSGGGWDNAKKLVEDGLHGGKGSEAHAATVIGDTVGDPFKDTAGPAINPLI
                     KVMNLVSVLIAPAVVALTYGDSASDTGRVLIAIAAVAIVAVAVVVAKRRPVVIGSEPQ
                     KAAV"
BASE COUNT         1322 a         3542 c         3588 g         1302 t
ORIGIN      
        1 gacgcggcgg cagggagcag agcgatggcc gtgagcggag ccgggctgag cgtgcgcccc
       61 tccccgcccc gtcacgacgt acggcgcgtc gcggcctccg ccttcttcgc cttcttgtag
      121 tcccggaccc gctgcagcga ctccgcgtcg accacgtcgg ccaccgatcg gtagccctcg
      181 agcgcgtagt cgccggcggc cttctcccag ccgtccggcc gtacgccgcg ctgcttggcc
      241 agcagcgcca cgaagatcgc cgccttctgc ttgccgaacc cgggcaacgc ctgcacccgc
      301 ctcaacaggt ctgccccggt ggcggcctcg gtccagatgc gtgaggcgtc gccgtcgtag
      361 tcgtcaacaa cgacccgcgc cagctcctgc aggcgcgccg ccatcgatcg tgcgtaacga
      421 tgaacagccg gcggtgtcgc gcacagcgcc gcgaacgcgt cggggtcggc gcccgcgatc
      481 gcggccggct ccagggtccc gaaccgctcc aacaccttcc atggaccgcg gaatgcgtgc
      541 tccatcggat actgctggtc gagcatcatt gcgctcacga gcgcgaacgg gtcctgctcg
      601 aggacagcgt cggcctcagg caccccggtc agctgcaacg acatgcgacc atccttgctc
      661 atccttcgcc tccgcgatct gccctccggg gagctcaaga cctcgggcga agtgcgcgtc
      721 cacgtaggcg atgtcgcgca ggtaggtgtc cgcatggcct gcctcgatgt gcgcggcgaa
      781 ccgtcggttg ccaccagccg cggcgtcacg catgtggtcc accaaccacc tcagccgcgg
      841 gccgatcgtc tcgagcgcgt cgcggcgctc ccgcgccgta cagccgtagg agtcgaggaa
      901 cagccgcgcc cgccgagcct gcgcgggtgg atccccgaac ccgtcagcgt tgccaggcgc
      961 ggtcaggggc gcgaagcggt agatggcata ggccaggtcc caccgacgcg ggcccggcct
     1021 ggcgaagtcg aagtcaatca ctcccaccgc ctcttggccc cgaaacacgc agttgtacgg
     1081 cgcgaagtca ccgtggcaga tcacttcgac cggctcgagc ggcaccagct gccatggcag
     1141 cgaggactcg cgcgtgagcg gcaccgaggc gtcatgcagg gaacgcagca gtcgggctgc
     1201 cgtgatcaaa gccgcatcgc tgcggatggc agccgacaac gggtagctgc cgacgtcgcc
     1261 gggcaggaag tcgaagacgt cccgaccctg gtcgtcgaca ccgcgccacg ccggggcctg
     1321 cgagacgccc gcggcccgca gccggtcgag cagcacgcga acggccgggg tccacgggcg
     1381 agccggtcga cggacggtgg cgcccacccg aacgacccgt gtcgtgccac ccccgggcag
     1441 ctcaacctct tcgagtgccc ggtcatcgct ggagggcatc tcgatcaggc cgcacggccg
     1501 gggtggtcgc gcaaccaggc gagcacgtga tcggcatgct cgtgcggcgg gaacaccggg
     1561 tagaaggcgc gtgcgatgac cccgtcgcga acgatgaggg tcagccgctt gagcaggacc
     1621 tggccggcga tcgtcatcgt gggcaggtcg agggcgcggg ccagctcaag gtccgcgtcg
     1681 ctgagcagtg ggaacggcag gtgcagccgg gctgcggcct cttgctggta gtcggtgtcc
     1741 tgcgtggaca cccccagcac ggcggccccg agcgcgccca gctcgcgcgc gtggtcgcgg
     1801 aaggagcacg actcgggagt gcaaccccgg gctccgggga tgagatccca atcgggagtg
     1861 agaggcggct cgcccggccg cccggtccgc gggtaggcat agaggacaag ggtttcgaac
     1921 ccgatgggtg ggcgaccgac gggcagccgt tcgccggtgg tggccggcaa ggtcagcgcg
     1981 ggcatcgcac ggcccggcag atgtgccgcg gcaccgtcgt cgtgcggccg gggcaggtcg
     2041 tcgggcaccg acatcgggtc acgtgcagca tcggtcacgg cagccctccc ccggccgacc
     2101 tcgtcgtcga caccagcccc cggggagcgc gacacggatc accggggacg ccggagccgc
     2161 ctcggggaat cgaaccccgg acctacgcat tacgagtgcg tcgctctgcc atctgagcta
     2221 aggcggcgtg tgcccgcagc cgccgtacgc cggccggatg cacggggcag gagtctacag
     2281 gcgcgggccc gcccgagccg accccgcccg agccggccgg tcagtcgcag cgggtgccct
     2341 cggccggcgt ctcacccttg agcaggtagg cgtcgaccgc ggagtcgacg cagtcgctgc
     2401 cgcgccggta ggcggtgtgc ccgtcgccct cgtaggtcag cagcacgccc gagtcgagct
     2461 gctcggccag cccctgcgcc cagacgtagg gcgtcgccgg gtcgcgcgtc gtgcccacca
     2521 ccaggatcgg tgcggcaccg gcggcgcgga gggcaccggg cgccgactcc gccttgaccg
     2581 gccagtagtg gcacggcagc gacgaccagg cgatgtaggc gccgaacagc ggcgaggcct
     2641 tctcgaaccc cggctgggca gcggccagct gctcgggccc gtcgggctcg ggccggtcga
     2701 ggcagttgac ggcgtagatc acgtcgttct ggttgctgcc gtagcgcccg tcggtgcccc
     2761 ggtcggcgta ggcgtcggcc agcccgagca gcggcctgcc gtcaccggac aggcccgcct
     2821 tcaaggcgct gcgcagctgc ggccagaaca cctcgtcgta catcgcgaag accacaccga
     2881 gtacggcgag ggcctgggtg agctggcggt cgccggccgg cagcgggcgg cggtcgaccg
     2941 cggcgaggaa gtcggccagc tgcgcccgag cgtctgcctc gtcgccggtc agcgggcagg
     3001 tgcgcttgcc caggcagtcg gccaggaacg cgctgaacgc gacctcgaac cccttggcct
     3061 gtcccaggtt gatctcggcg ctggtcgagc gcgggtcgag cgcgccatcg agcaccagcc
     3121 ggccgacccg ccacgggaac atctcggcgt aggtggcgcc gatgaacgtg ccgtaggact
     3181 tgccgaggta gtgcaggtcg tcgtcgccga gcgccgcgcg cagcacgtcg aggtcgcggg
     3241 cgacgtcctc ggtgccgagg tgggccagca cctccgggtt gcgcgcctgg cagcgctgcc
     3301 ccagctgctg ggacagctcg acgagccgag ccacctcggc ggcgtcgtcg ggggacgcgt
     3361 cggcggcgag gaacacgtcg gtctcgcggt cgtcgaggca gcgcaccggg tcgctggtcg
     3421 ccacaccgcg cgggtcgaac ccgacgacgt catagcgctc acgcaccggc gagctcacca
     3481 cggcggtggc atagccggcg tactcgacgc ctgacccgcc cggcccgccg gggttgagca
     3541 cgagtgagcc gagccggggg ccggaggccg gcagccggtt gacggcaagg ccgagcgtgg
     3601 gcccgtcggg gtcggcgtag tcgaccggca cggtcagcgt ggcgcactcg aagccggccg
     3661 tgccggaggc cgactcgcac gaggcccacc gcagcgtctg ctggtagtag gaggcgagcg
     3721 cggggtcggt ggcggggtcg tcggccggcg cggacgccgg tggcgccggc cgggtctcgg
     3781 ccccggtcgg gccggtgtgg gtgctggggc tcggcacgcc ggtcgggtcg ccgctgcagc
     3841 cggtgaggag caccccgagc gccagggtgg ccgcggcgag ccgcgtcggc cgggtcacgg
     3901 ttgcctcagc cgcaaggcca tcgcctcgac cgccagcaac ggggcgacgt tggcctcgac
     3961 cgcctcgcgg gcggccagga tcgcctccat ccgccccaac gtgacctccg gggtgctcgt
     4021 ggccgccacc cggtcgatct gcgggcgcag ctcggcgttg gtgagtccga cgtcgtccgc
     4081 accgacctgc acggccagca cgtcgcggta gaacgtcacc aggtcggtca gcgcccggtc
     4141 gagggcgtcg cgcttgaccc gggtggcccg ctgcttctgc gccttctcga ggtccttgag
     4201 cgcggcccgc gccgagccgg acatggcccg gccggtcgtc ccctccccga gcgcgcggcc
     4261 gagcgcctcg gcctcgcgca cgtcgagctc ggccgtggtc gcggctgcct cctcggtcgc
     4321 cgcctcgacc agcgcggcgg ccgcggccac cgcctgggcg acgccggtga cgatcgtcgg
     4381 gacgcgcagc acctcggccc ggcgcaaccg ggccgactcg tcctgggcca gccgccgagc
     4441 ccggccgatg ggcccgccca ccgcgcgcgc ggcgaacgcg gccatcgccg ggtcgacgcc
     4501 gtcgcgggcg gtgaggaagg ccgcgaccgc cgcgaccggc ggcgtgcgca gcagcacgtg
     4561 ccggcaccga gaacggatcg tgggcaggac gtcttcgggc gagggggcgc agagcagcca
     4621 cacggtgcgg gccgcgggct cctcgaccgc cttgagcagg gcgttggcgg cctgctcggt
     4681 gagccggtcg gcgtcctcga cgatcacgac ctgccaccgg ccgccggccg ggtggcgtga
     4741 ggcgcgcagc acgagctcgc gggcgtcctt gacgctgatc gtcagcgcct cggtgctcac
     4801 gacctcgagg tcggcgtgcg agccggagcg cacggtgcgg caggcggtgc actcgccgca
     4861 gccgccgtcg aggcactgca gggccgcggt gaaggcccgg gccgccgtcg aacggcccga
     4921 cccgggcggg ccggtgatga gccaggcgtg ggtcatggcc gaagcgggtt gtcccgccag
     4981 cacaccggcc gcgtcggcag cggcggcccg cagcgtctca accacccgct cctgcccgac
     5041 caggtcgtcc cagacgctca cgccgacccc tcgcgggccg caagcagctc ggtcacccgg
     5101 tcgcgcacct gttcccagac ccggtcgggc tcggcgctgg cgtcgacgac gaggtagcgg
     5161 gccggctcgg cggcggccag ccgggcgaac gcgtcgcgga cccgggcgtg gaaggccagc
     5221 ggctcggcct cgagccggtc gggcggacgc tcgaaccggt gcagccccac ctgcggggcc
     5281 acgtcgagca gcacagtcag gtcgggccgc agcccgtcgg tggcaaaggt ggacagcgcc
     5341 tcgaccgcgt cggcgccgag gtcgcgcccg gcgccctggt aggcgatcga cgagtcgacg
     5401 aaccggtcgg tgaccaccac cgcaccgcgg gccagcgcgg ggcggatgac ccgggtgacg
     5461 tgctcggccc tgtcggcggc atagagcagt gcctcggccc gcgcggacgg gtcgggaccg
     5521 tcgaggtgaa gcagcatcgc gcgcagcgcc tggcccagct cggtgtcgcc gggctcgaac
     5581 gtcagcaccg tctcgcggcc cgccgcctcc agccagccga ccagccgctt ggcctgggtc
     5641 gacttgccgg ccccctcgcc gccctcgaag gcgatcagca gcccgcgcgg gtcggctagg
     5701 gcaggcgtgg gctcgggcac gcggtcagcc taacgacgcc gtacgacgtt gcgggtcagc
     5761 gcttgggtgc ggccttcttc gtcgtacgac gggccgcggt cttgcgcgcc ggcttcttgg
     5821 ccgggccctt ggcccgcttc tcggccaaca gctcggaggc ccgctcgatg ctcaccgtcg
     5881 tcgggtcgtc gcccttgcgc agcgtcgcat tggtctcccc atcggtgacg taggggccga
     5941 agcggccgtc cttgaccacg accggcttgc cggtgaccgg gtcggcgccc agctccttca
     6001 gcggcgcggc ggcggcccgg cgaccgcgct gcttgggctg ggcgtagatc gccagcgcct
     6061 cctcgagggt gatcgtgaac agctggtcct cggagtccag cgaccgggag tcggcgccct
     6121 tcttgaggta gggcccgtag cggccgttct gcgcggtgat ctcctcgccg gactccgggt
     6181 cggcgccgac cacccgcggc aaagtcagca gccgcagcgc gtcgtcgagg gtcacggtct
     6241 ccagcgtcat cgtcttgaac agcgacgcgg tgcgcggctt ggccgacttg ggggcgtcct
     6301 cgggcagcac ctcggtgacg tagggcccga agcggccggc cttggccacg atctcccgac
     6361 cggtggccgg gtcgacgccc agctcgcggt cacccgatgg cgcggcgagc agctcctcgg
     6421 cccgctcgac ggtcagctcg tcgggggcca ggtcgtcggg caccgacgcg cgttcctcgc
     6481 cgcgctcgac gtagggcccg tagcggccca cccgcagcac gatctcgttg ccgaccggga
     6541 tcgtgttgac ctcgcgggcg tcgatgtcgc ccaggccctc gaccagccgc ttgagcccct
     6601 cgagctggtc ggtgccgccg ttgccgaagt agaaccggga cagccaggcc acccggtcct
     6661 cgttgccgcc ggcgatccgg tcgaggtcct cctccatcgt ggcggtgaag tcgtagtcga
     6721 ccagccggcc gaagtgccgc tccaacagcc cagtcaccga gaacgccagc caggtcggca
     6781 ccagcgcggt gcccttcttg aacacgtagc cccggtcgag gatcgtgccc atgatcgagg
     6841 cgtaggtcga cggccggccg atgccgcgct cctccagcgc cttgaccagc gtggcctcgg
     6901 tgtagcgggc cggcggcgtg gtctcgtgcc cgtctgcctc cagcccccgc tcggtcagcg
     6961 cgtcgccggt gcgcagcgcg ggcagccgac gctcgcgaag atcactaccg tcgcggtcgg
     7021 cacgctcggt gtcgtcgtcg acgccctcct cgtaggcggc gaggaaccca cggaaggtga
     7081 tcaccgtgcc ggaggccgag aactcggcgt cccgcccgtc ggcggcgacc gcgcccagcc
     7141 gcacggtggc cgtggtgccg accgcgtcgc gcatctgcga ggcgatggtg cgcttccaga
     7201 tcagctcgta gagcgcgaac tcgtcgccgc gcagctcgcc gctgacctgc ccgggggtgc
     7261 ggaacacgtc gccggccggg cggatcgcct cgtgcgcctc ctgcgcgttc ttgaccttgc
     7321 gctcgtagcg gcgcggggcg tcgggcacgt ggtcggcccc gaacagctcc cgcgcctgcg
     7381 agcgcgcggc ctgcagcgcc gactccgaca gcgtggtcga gtcggtgcgc atgtaggtga
     7441 tgtagccgtt ctcatagagc cgctgcgcga cccgcatcgc cacctgcgag gacaaccgca
     7501 gcttgcggct ggcttcctgc tgcagggtcg aggtcatgaa cggcgcggcc ggcttgcgcg
     7561 tgtagggctt gtcctcgacg gaacgtacgg cgaactcgcg cccgcccaac gcgtcggcca
     7621 acgaccgcgc gccggtctcg tcgagcaccg cgacgtcgcg cccggccagc accgagccgt
     7681 cgcggccgaa gtcacggccg gtcgccaccc gtcgcccgtc gacggcgacc agccgggcga
     7741 tgaacggggt ctgctcccca gcggcggcga cgaaccggcc ctcgacgtcc cagtaggacg
     7801 cgacccggaa cgcgatccgc tcgcgctcgc gctcgaccac cagccgggtc gccaccgact
     7861 ggactcgccc ggcggacagc ccggcgcgga ccttgcgcca gagcaccggg gagacctcgt
     7921 agccgtagag ccggtcgagg atgcgccggg tctcctgggc gtcgaccagc gactggtcga
     7981 gctcgcgggt gtgctcgacc gcctcgcgga tcgcgtcgcg ggtgatctcg tggaagacca
     8041 tccggcgcac cggcacccgg ggcttgagca cctcgcgcag gtgccaggcg atcgcctcgc
     8101 cctcgcggtc ctcgtctgtg gccaggtaga gctcgtcggc ctcggcgagc agcttgcgca
     8161 gctcggcgac cttcttcttc ttgtccgcgt ctaccacgta gaacggctcg aagccgttgt
     8221 ccacgtcgac ggcgaacttg ccgaaccggc ccttcttctc cgccgccggc agctccgacg
     8281 gcgtgggcag gtcacggatg tgcccgatgc tcgcctcgac gtcgtagccg gcgccgaggt
     8341 agcccttgat cgtcttcgcc ttggcaggcg actcgacgat cacgagccgg cggttgccgg
     8401 cctggctctt cgcgggcacg gggcctcctg ggtcatcaca cggaactggc ggagtgtcgc
     8461 cctcccatgt ggggtgctgt caaaccgccg caccgtgagg ggcagcgatg agggcccgct
     8521 ccagcactcg gagcgggccc tcacgtcgta cgacgaacgc ggtcagaccg ctgccttctg
     8581 gggctccgac ccgatgacga ccgggcggcg cttggccacc acgacggcca ccgcgacgat
     8641 cgccaccgcc gcgatggcga tgagcacccg gccggtgtcc gaggcggagt cgccgtaggt
     8701 cagggcgacg acggcgggcg cgatgagcac cgacaccagg ttcatcacct tgatcagcgg
     8761 gttgatcgcc gggccggcgg tgtccttgaa cgggtcaccg acggtgtcgc cgatgacggt
     8821 ggccgcgtgg gcctccgagc ccttgccgcc gtgcaggccg tcctcgacga gcttcttggc
     8881 gttgtcccag ccaccgccgg agttggccag gaagatcgcc atcagcgtgc cggtggcgat
     8941 ggcgccggcc aggaagccgg ccagcgggcc gacgccgagg ccgaagccga ccgcgatcgg
     9001 ggcgagcacc gcgagcaggc cgggggtggc cagctcacgc agcgagtcgc gggtgcagat
     9061 gtcgacgacc cggccgtact ccggacgctc ggtgccgtcc atgatcccgg ggtggtcggc
     9121 gaactgctgg cgcacctcga agaccacggc gccggcggcg cgggccaccg cgttgatcgc
     9181 caggccggag aacaagaaca ccacggcggc gccgaggatg atcccgacca gggtgttggg
     9241 gctgaacacc agcgagttgt tgaggaagtc ggtgacgacc tcgccgacac cgtcggacgc
     9301 cgtacgcacg gcgtcggtgt aggagccgaa cagcgcggtc gcggccagca cggccgtcgc
     9361 gatcgcgatg cccttggtga tcgccttggt ggtgttgccg accgcgtcga gctcggtgag
     9421 gatctgcgcg ccctcgccgt gcacgtcgcc ggacatctcg gcgatgcctt gcgcgttgtc
     9481 ggagaccggg ccgaacgtgt ccatcgccac gatcacgccg accgtggtga gcaggccggt
     9541 gccggccagg gccaccgcga acagcgagat gatgaccacg ccgccgccga gcaggtaggc
     9601 gccgtagacc gccgcgccga tgaccaccgc ggtgtagacg gccgactcca gaccgagcga
     9661 gatgccggag aggatgacgg tggccgcgcc ggtgagcgag gtcttgccga tgtcctggac
     9721 cgggcgcttg tcggtgccgg tgtagtagcc ggtc
//
LOCUS       DATIBB010000079         5651 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_1486968, whole genome shotgun sequence.
ACCESSION   DATIBB010000079 DATIBB010000000
VERSION     DATIBB010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 5651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5651
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1486968"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..543)
                     /locus_tag="VLC50_02800"
     CDS             complement(<1..543)
                     /locus_tag="VLC50_02800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="amidinotransferase"
                     /protein_id="HSF26425.1"
                     /translation="MCAPEHFRVDYAINPWMDPAAVVDVDLARAQWDRLVEVLEQAGA
                     RIERIAGRTDCPDMVYAMNYGLVDGDRVAMTTFRHHERAAEAEAAARWFADRGLRPAK
                     IVVGAGTAFEAGDAFLFGDALLVSAGPRTDRAAHAELARRLDVRVVPVEIVHPAYYHL
                     DLSFCPLDETKAIIAPGAWSP"
     gene            724..1395
                     /locus_tag="VLC50_02805"
     CDS             724..1395
                     /locus_tag="VLC50_02805"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HSF26426.1"
                     /translation="MREVAIDRSSTAQRVADALRTMMFEGELTGDQPLRETALAGSFG
                     VARSTVREALSILWLEGLVTRSPGRGAQVAALSPSDVEEIFTARAILEGAGVDAARQA
                     SPEAVQALRDALDDYDAAARAGNRSAATVAHLDFHSALVGLLGSRRLLAASAALTRDL
                     RLALAAVGRRAGDAPEQIQAHRRLLELVAAGDTRAAAAELASHLDAAKASLVAQTRGA
                     SESDR"
     gene            1392..1943
                     /locus_tag="VLC50_02810"
     CDS             1392..1943
                     /locus_tag="VLC50_02810"
                     /inference="COORDINATES: protein motif:HMM:NF018984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CGNR zinc finger domain-containing protein"
                     /protein_id="HSF26427.1"
                     /translation="MSDLSTYGDIAVDLVNSGHPRDPDHDRLCRPADVLRLLGRDAYR
                     FEGQVGPPEVAALRAARERFRAVFTKVDAGDAAGAVGVLNTILAGARIRPELSDHDGE
                     GWHLHLSRGRESPSGVYVATAAMGLAARIAAVGLDRLGVCQAAPCVRVYLDTSTNRSR
                     RYCCERCATRANVAAYRARRRDA"
     gene            complement(1951..2298)
                     /gene="sodX"
                     /locus_tag="VLC50_02815"
     CDS             complement(1951..2298)
                     /gene="sodX"
                     /locus_tag="VLC50_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003953999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0016485 - protein processing [Evidence
                     IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-type superoxide dismutase maturation
                     protease"
                     /protein_id="HSF26428.1"
                     /translation="MLGRTGSRRPAWPLGRVAVAGASMLPTLHPGDWLVVRWGAHVRP
                     GDVVVARRPDRPGLLLVKRVVRREPGGWWLVGDNPGASDDSRLFGPVADDLVLGRVLG
                     RYWPLGRRRVFPV"
     gene            2396..2797
                     /gene="sodN"
                     /locus_tag="VLC50_02820"
     CDS             2396..2797
                     /gene="sodN"
                     /locus_tag="VLC50_02820"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020575800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase, Ni"
                     /protein_id="HSF26429.1"
                     /translation="MLTRLFAPRITVHAHCDLPCGVYDPAQARIEAQSVKAIMEKYAA
                     NEDPEFRTRALIIKEQRAELVKHHLWVLWTDYFKPAHFEKYPQLHTLFNEATKLAGAA
                     GGKGSTDVAKGDELLAKIDEIATIFWETKKA"
     gene            2973..3203
                     /locus_tag="VLC50_02825"
     CDS             2973..3203
                     /locus_tag="VLC50_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006591439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF26430.1"
                     /translation="MPSVQIKDVPDDVHSVLRARAAAAHQSLQEYLRSRLIAEARRPT
                     LDEIFDRAAGRSGGSVSLEDAVAIVRDDRARR"
     gene            3175..3591
                     /locus_tag="VLC50_02830"
     CDS             3175..3591
                     /locus_tag="VLC50_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007102185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HSF26431.1"
                     /translation="MCATIVLVVDASVVAVALADDGADGESVRARLRVEDLTAPELLD
                     LEVASVLRTRTMVGALDPRRARLALADLAAMPLQRAPHLPLLQRAWELRHNLTVYDGA
                     YVALAEALGVDLLTGDRRLARTPGPRCRIELLGPHI"
     gene            3761..5482
                     /locus_tag="VLC50_02835"
     CDS             3761..5482
                     /locus_tag="VLC50_02835"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017592712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase"
                     /protein_id="HSF26432.1"
                     /translation="MTDTTRVQAAISTSRVTRQGHGGDLAVAVARAHGITAMFTLSGA
                     HVFPLYDAAVREGSGMTLVDVRHEQTAVFAAEATAKLTRVPGLAVLTAGPGVTNGISA
                     VTGAWFNGSPLLVLGGRAPDYRWGTGTLQELDQPPLLASITKLAETVHETAAIPARVD
                     AALTTAATPHRGPVFLDVPMDVLYDRAEAAVPAPAPREAVAPDPDALAAIADLVVQAL
                     HPVLVLGSDVWLDGAGHAARRLAETLRLPVIANGMGRGVLPRGHELLVTRARSRVLAG
                     ADLVIVAGAPLDFRLGYGSFGGRPGTEGQQAAPARVVHLADSPGQVATHVALAGSAAG
                     DLTLVLDGLRGAWERHPAAPAQTATATGWLTTVQAATLAAVERDRVVLGADTDPIHPA
                     RVYGELLPRLEPDAVVIGDGGDFVSFAGKYVEPAAPGGWLDPGPYGCLGAGLGAAIAA
                     RLARPAAQVVLLLGDGAAGLSLMDVDTLVRHRLPVVMVVGNNGVWGLEKHPMRMLFGY
                     DVAADLAPGTRYDEVVRALGGAGETVTKPGEIGPALDRAFASGVPYLVNVLTDPEVAY
                     PRATTGI"
     gene            complement(5517..>5651)
                     /locus_tag="VLC50_02840"
     CDS             complement(5517..>5651)
                     /locus_tag="VLC50_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pili twitching motility protein PilT"
                     /protein_id="HSF26433.1"
                     /translation="GMQTFDQHLVALIRDGVVTLEAAMLAASNPHDLTVELRRLGLVA
                     "
BASE COUNT          783 a         2124 c         2014 g          730 t
ORIGIN      
        1 cgggctccac gcccccggcg cgatgatcgc cttggtctcg tcgagcgggc agaagctgag
       61 gtcgaggtgg tagtaggccg ggtgcacgat ctcgaccggc accacccgca cgtcgagccg
      121 gcgggccagc tcggcgtggg cggcccggtc ggtgcgcggt ccggcggaga ccagcagggc
      181 gtcaccgaac aggaacgcgt cgccggcctc gaaggccgtg ccggcaccga cgacgatctt
      241 ggccgggcgc agaccgcggt cggcaaacca acgggcggcc gcctcggcct cggcggcccg
      301 ctcgtggtgg cggaacgtcg tcatcgcgac ccgatcaccg tcgaccaggc cgtagttcat
      361 cgcatagacc atgtcggggc agtcggtgcg cccggcgatg cgctcgatgc gcgccccggc
      421 ctgctcgagc acctcgacga gtcgatccca ctgggcgcgg gccaggtcga cgtccacgac
      481 ggcggcaggg tccatccacg ggttgatcgc gtagtcgacc cggaagtgct cgggtgcaca
      541 catgagatag cgacgacccc agcacagcgg atgcagcacg acgagccccc aaagcgacgg
      601 aagatgtcag attgtcggac aacctgacca tgctgcgacg atcgcgcttc aaggtcaagg
      661 accgccggtc taccctgtgc gtcgttccgg gcccacccca accgagagcc gcggaggccg
      721 gcagtgcgcg aggtcgccat cgaccggtcc agcaccgcac aacgagtggc cgacgcgctg
      781 cgcacgatga tgttcgaggg cgagctgacc ggcgaccagc cgctgcggga gacggccctg
      841 gccggctcgt tcggggtggc ccgctcgacg gtgcgcgagg cgttgtccat cctgtggctg
      901 gagggcctgg tcacccggtc ccccggccgc ggggcgcagg tcgcggctct ctcgcccagc
      961 gacgtggagg agatcttcac ggcgcgggcg atcctcgagg gcgccggggt ggacgccgcc
     1021 cggcaggcca gccccgaggc ggtccaggcc ctgcgtgacg ccctggacga ctacgacgcg
     1081 gccgcgcgcg ccggtaaccg gtcggcagcc accgtggccc acctcgactt ccacagcgcg
     1141 ctggtcggac tgctcggcag ccgccggctg ctggcggcca gcgccgccct gacccgcgac
     1201 ctgcggctgg ccctggccgc ggtcggccgg cgggccggcg atgcccccga gcagatccag
     1261 gcgcaccgcc gccttctcga gctcgtcgcg gcgggcgaca cgcgggcggc cgccgccgag
     1321 ctggccagcc acctggacgc ggccaaggcg tcactggtcg cccagacccg gggcgcctcg
     1381 gagagcgacc gatgagcgac ctgtccacct acggcgacat cgccgtcgac ctcgtcaaca
     1441 gcggccaccc gcgcgaccca gaccacgacc ggttgtgccg gcccgccgac gtgctccggc
     1501 tgctcgggcg cgacgcctac cggttcgagg gccaggtcgg cccgcccgag gtggccgccc
     1561 tccgggcggc gcgcgagcgc ttccgggcgg ttttcaccaa ggtcgacgcg ggcgacgccg
     1621 ccggtgcggt cggcgtactc aacacgatcc tggccggtgc ccggatccgg cccgagctct
     1681 ccgaccacga cggcgagggt tggcacctgc acctgtcgcg cgggcgggag agcccgtccg
     1741 gcgtctacgt cgccacggcc gcgatggggc tggccgcccg gatcgccgcg gtcgggctgg
     1801 accggctcgg ggtctgccag gccgcaccgt gcgtgcgggt ctacctcgac accagcacca
     1861 accggtcgcg ccgctactgc tgcgagcggt gcgcgacccg ggccaacgtc gcggcctacc
     1921 gggcccgccg ccgcgacgcc tgacgtcggg tcacaccggg aacacgcgtc gccgacccag
     1981 cggccagtag cgcccgagca cccggcccag caccaggtcg tcggccaccg gcccgaacag
     2041 ccggctgtcg tcgctggcgc ccgggttgtc ccccaccagc caccagccgc ccggctcgcg
     2101 gcgtacgacc cgcttgacca gcagcaggcc gggccggtcg ggccgccggg cgaccaccac
     2161 gtcccccggt cgcacgtgag caccccagcg caccaccagc cagtcgcccg ggtgcagcgt
     2221 gggcagcatc gaggcgcccg ccaccgcgac ccggcccagc ggccacgccg gacgccgcga
     2281 gccagtcctg ccgagcacgg cacctcctct cgacgtgatc gctccccggg gtagtgtcga
     2341 cgctgagcca gtagagatca aaagatcaca gcacccgacg aagggagtga cgcgcatgct
     2401 cacgcgcctg ttcgccccac gtatcacggt ccacgctcac tgcgacctgc cctgcggggt
     2461 ctacgacccg gcccaggccc ggatcgaggc gcagtcggtc aaggcaatca tggagaagta
     2521 cgccgccaac gaggaccccg agttccgcac ccgggccctg atcatcaagg agcagcgggc
     2581 cgagctcgtc aagcaccacc tgtgggtgct gtggaccgac tacttcaagc ccgctcactt
     2641 cgagaagtac ccgcagctgc acacgctgtt caacgaggcc accaagctgg ccggcgcggc
     2701 cggcggcaag ggcagcaccg acgtggccaa gggcgacgag ctgctcgcca agatcgacga
     2761 gatcgcgacg atcttctggg agaccaagaa ggcctgaggc gccgtgacgc ctcggcctgc
     2821 aagacgctgg aacaccactt cggcgctgcc tcggtcttac cgcccccgca agggctccgc
     2881 gtgaactgag cgcagcgccg aaggcatttc aggcggcgcc ctccacctag gcacgccttg
     2941 cctgtcggtg ccgatggcgc caaaatggcg ccatgcctag cgtgcagatc aaggacgtgc
     3001 cggacgatgt ccactccgtg ctccgtgcgc gcgcggccgc tgcccatcag tcattgcagg
     3061 aatacctgcg ctcccggctg atcgcggagg cacgccggcc caccctcgac gagatcttcg
     3121 accgagcagc cggacgctct ggtggctccg tctccctcga ggatgcggtg gcgattgtgc
     3181 gcgacgatcg tgctcgtcgt tgatgccagc gtcgtcgcgg tcgcactggc agacgacggc
     3241 gccgacggag agtctgtgcg ggctcggctg cgcgtcgagg acctgacggc ccccgagctg
     3301 ctcgacctcg aagttgcctc ggtgctgcgt acacgaacaa tggtcggcgc gctggacccg
     3361 aggcgagcgc ggcttgcctt ggctgacctc gcggctatgc cccttcagcg tgcaccacat
     3421 ctgcctctgc tccaacgagc atgggagctg cgacacaatc tcaccgtcta cgacggtgcc
     3481 tatgtcgcgc tcgcagaggc actgggggtt gacctgctga ccggcgaccg gcgactcgct
     3541 cgcacaccgg gcccgaggtg tcggatcgaa ctgcttggcc cacacatcta gtcgcgccat
     3601 ctcggtcggc gcctgcatcg cccgtctcgc cgggccggtg ctgctgctgg tcggcgagga
     3661 cagtcccgcc gaggtcaagg ccgaccgcga ggccgtagca ccgacccgca gggcttcgca
     3721 gagacggtct tggcgttcct gcgcgggtag gttcggcctc gtgaccgaca ccacgcgcgt
     3781 ccaggccgcc atctccacga gccgcgtgac ccgccaaggc cacggcggtg acctcgcggt
     3841 ggcggtcgcc cgggcccacg gcatcacggc catgttcacg ctgtcaggcg cacacgtctt
     3901 tccgctgtac gacgcggcgg tgcgcgaggg cagcgggatg acgctggtcg atgtccggca
     3961 cgagcagacc gcggtgttcg ccgccgaagc caccgccaag ctgacccgcg tgccaggcct
     4021 ggcggtgctc accgccgggc ccggcgtgac caacggcatc tccgcggtga ccggcgcctg
     4081 gttcaacggc tcaccgctgc tggtcctcgg cggccgggcg cccgactacc gctggggcac
     4141 cggcaccctg caggagctcg accagccgcc gctgctggcc tcgatcacca agctggccga
     4201 gacggtgcac gagaccgcag ccatcccggc ccgggtcgac gccgcgctga cgaccgccgc
     4261 gacgccgcac cgcggcccgg tcttcctcga cgtgccgatg gacgtcctct atgaccgggc
     4321 cgaggccgcc gtgccggcac cggctccgcg cgaggcggtc gcgcccgacc ccgacgcgct
     4381 cgccgccatc gccgacctgg tggtccaggc cctgcacccg gtgctggtgc tgggctcaga
     4441 cgtgtggctc gacggcgccg ggcacgcggc acgccggctg gccgagaccc tgcggctgcc
     4501 ggtgatcgcc aacgggatgg gccgcggcgt gctgccgcgc gggcacgagc tgctggtcac
     4561 ccgggcccgc tcccgcgtgc tcgccggcgc cgacctggtc atcgtggccg gcgccccgct
     4621 cgacttccgg ctcggctacg gcagctttgg cggcaggccg ggcaccgagg gccagcaggc
     4681 cgcccccgcg cgcgtcgtac acctggccga ctcccccggt caggtggcca cccacgtcgc
     4741 gctcgccggg tcggccgccg gtgacctcac gttggtgctc gacgggctgc gcggggcctg
     4801 ggagcgccac ccggccgccc cggcgcagac cgccaccgcg accggctggc tgaccacggt
     4861 gcaggccgcg acgctggccg cggtcgaacg cgaccgggtc gtgctggggg ccgacaccga
     4921 cccgatccac ccggcccgcg tctatggcga gctgctgccc cggctcgagc cggacgcggt
     4981 cgtgatcggc gacggtggcg acttcgtgtc cttcgccggc aagtacgtcg agccggccgc
     5041 gccggggggc tggctcgacc cgggccccta cggatgcctg ggggcggggc tcggcgccgc
     5101 gatcgcggcc cggctggcgc ggcccgcggc gcaggtggtg ctgctgctcg gtgacggcgc
     5161 ggccggcctg tcgctgatgg acgtcgacac gctggtgcgc catcggctgc cggtggtgat
     5221 ggtggtcggc aacaacggcg tgtgggggct ggagaagcac ccgatgcgga tgctgttcgg
     5281 ctacgacgtg gcggccgacc tggcgcccgg cacccgctac gacgaggtgg tgcgggcact
     5341 cggtggcgcg ggcgagacgg tcaccaagcc gggcgagatc ggcccggcgc tcgaccgggc
     5401 gttcgcctct ggcgtgccct acctggtcaa cgtgctcacc gaccccgagg tggcctaccc
     5461 gcgggcgacc accggcatct gagcgccggc ccgacgagcc gtcgcggccg gaccggtcag
     5521 gcgaccaggc cgagccggcg cagctccacg gtcaggtcgt gcgggttgga cgcggcgagc
     5581 atcgcggcct cgagcgtcac cacgccgtcg cggatcagcg cgaccaggtg ctggtcgaag
     5641 gtctgcatcc c
//
LOCUS       DATIBB010000080         8406 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_172403, whole genome shotgun sequence.
ACCESSION   DATIBB010000080 DATIBB010000000
VERSION     DATIBB010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 8406)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8406)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8406
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172403"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..891
                     /locus_tag="VLC50_02845"
     CDS             <1..891
                     /locus_tag="VLC50_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018216739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HSF26434.1"
                     /translation="IRRSFSSRLATVLVIALTVLWTIPTFGLLVTSFRPQSEVATSGW
                     WTFFTNPEVTLGNYYTVLISGGFNVPGGLMPFFVNSFVIAIPATLIPLTIAAMAAYAL
                     AWVNFRGSDILFFTIFALQVVPLQMALLPLLQFFNDGFSIGSVTLFPALHLSGSFFTV
                     WIAHTMFAIPLAVFLLHNFMAQLPRDIMEAARVDGASFFMSFRKIALPLSVPALAAFA
                     IFQFLWVWNDLLVALTFVGGQPTVAPLTARLSQLTGSLGSRWDLLTAGAFISIVVPLI
                     VFFALQRYFVRGLLAGSVKG"
     gene            complement(907..1434)
                     /locus_tag="VLC50_02850"
     CDS             complement(907..1434)
                     /locus_tag="VLC50_02850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF26435.1"
                     /translation="MAGLDVLRGGDPPRSRRRRWPWVLLVVLVAAALGLRWADGVVAE
                     REYDALLRAWTTAQGHTAYAERSVASIVEYASPQLQTAPADVRAGLRELVEQAAAKQL
                     PVLHESIGAVTRVGVLPWHHALQEARAACAAYLDGRVHRLEAVALNALALGQPAVAFD
                     ADLAETLALLHDAAP"
     gene            complement(1453..2817)
                     /locus_tag="VLC50_02855"
     CDS             complement(1453..2817)
                     /locus_tag="VLC50_02855"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012889988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008422 - beta-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0030245 - cellulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH1 family beta-glucosidase"
                     /protein_id="HSF26436.1"
                     /translation="MGPFVYGVATSAFQVEGATDADGRGPSIWDTFAAQPGAIVDGSD
                     GRTACGSYERWADDLDLLAELGVGAYRFSIAWPRVQPEGSGPINTPGLDYYDRVVDAL
                     CAKGIQPLPTLYHWDLPQSLEDDGGWPSRETALRFADYAAAVHGRLSDRVGVWATLNE
                     PWCSAFLGYAAGVHAPGRREPGAAYAAAHNLLLAHALAAATMREQHAESKVGLALNLT
                     TVHTDDLRAVEAARFVDAVRNRLWLDPLLDGRYPADLLEAVPVLEDPEVVDAGDLTLV
                     AGSADWIGVNYYNPLRVGSPEPGADDSDADPEVAAYPGALPLRFRPREPRTPMGWEVD
                     PGGLTDVLVELAARDPRLVLRVTENGAAYGDGVIGPDGRIDDADRAAYLQAHIAAVHA
                     ARAAGADVRAYLAWTLLDNFEWSHGYTQRFGLVAVEPGTLARVPKRSFAWYADLIRAG
                     APAG"
     gene            complement(2845..3267)
                     /locus_tag="VLC50_02860"
     CDS             complement(2845..3267)
                     /locus_tag="VLC50_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014740813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HSF26437.1"
                     /translation="MTRLLGSVIDAVVASLIVAALYLAWSGLAFVADPTGFRFPRPSF
                     GLVLGVGLGTLALYLAGCWWVTGRSYGSHVMGLRVINWRGTRPSLPVALLRAGMCVLF
                     PIGLFWVVVSPRNKSVQDLVVRTSVIYDWEPNHPSPQR"
     gene            complement(3330..4304)
                     /locus_tag="VLC50_02865"
     CDS             complement(3330..4304)
                     /locus_tag="VLC50_02865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF26438.1"
                     /translation="MDAQHPVPPLWQGARLVIGLSRRAAREVAAAAGRGVPRPGADRN
                     TELAVGVAAQAFEAAAGAIEIATGVAAAVGRGLRPLTWPLRVPLQRSAQALDRTRERG
                     RAELALAEPEATRLLDALLPRLVEAVLDRLDLTELVRRHVDLDRLVAHVDLNAAAARL
                     NVDAVAARLDLDAAAARLDIDRIAARLDIDRVAARLDLEQILRRLDLNALIASVDVDA
                     IASRIDLDAIVARLDLVDLAEDVIDRVDLAEIIRSSSGSVASEAVRDVRLQSIEADEA
                     VSGFMDRLMGRQRHPGHHRGDVPAPRQPIEEPARQAIEAPAPESTEES"
     gene            complement(4388..5965)
                     /locus_tag="VLC50_02870"
     CDS             complement(4388..5965)
                     /locus_tag="VLC50_02870"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HSF26439.1"
                     /translation="MDRLDVRVTRAMELAQSGAPREAVALADELLAHAVEAQRSDLVP
                     RLHYVRAVAHHSLGEHAQAISDCERLLTVAEAIDDQGWVSNARSLRASQYVLLGDIDE
                     GVRDLVAAQVALDGAGDTNPLVLSFAHTGLAIGFDLVRLYELALPHYLAPLELPDTPD
                     QQPESRAIDHLNLAEFHLRWGLELERVGDTGPLHQHAGTALEHARAAQREAKPLDRGR
                     WRHTADLLAGSALALSAAPAEAVELLRGAVDAVLALDLATEATFGLVFLSRALEDLGR
                     LDDAERAARFANDLLPPDADWPLVASVHDRHARLAAHRSGDPAAQVALHYAETMAREV
                     WRQRLRTLRGAEAQLTLERLRTEHLAAQRASHEDPLTGVANRRHLEQRLHELAETAPP
                     SPYPVSAIMLDVDSFKQINDTLGHQTGDEVLRAITRVLVEHARSSDVVARLGGDEFVV
                     LLTDTGPDEVAIVASRMLAAIRTTNWAAITRGEPVTVSVGSATTMSLRDGAELLALAD
                     DAMYVAKRAGGDQLHSI"
     gene            6235..6450
                     /locus_tag="VLC50_02875"
     CDS             6235..6450
                     /locus_tag="VLC50_02875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF26440.1"
                     /translation="MTSPRELAAGSSPSASKARSRTRLLRDRTDIRLQDPQALDEIDL
                     YGEVVIAASACEGPLSLEQLDRVLGLR"
     gene            6588..7208
                     /locus_tag="VLC50_02880"
     CDS             6588..7208
                     /locus_tag="VLC50_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016570948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HSF26441.1"
                     /translation="MRFRLAPRETSFYDMFATSANNLVTAAKLLKDMLSADAPGREEI
                     AVKMRAAEHAGDEATHAILRQLNGTFITPFDREDIYRLAARLDDCVDFMEAAVDLVLL
                     YKIDELPHGVADQVAVLERCAELTAEAMPRLRSMTDLSEFWIEVNRLENQADQVYRRM
                     LAEIFDGQYDALTVLKFKEVIDQLESAADAFENVANMIETIAVKES"
     gene            7212..8213
                     /locus_tag="VLC50_02885"
     CDS             7212..8213
                     /locus_tag="VLC50_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HSF26442.1"
                     /translation="MELLGLIAFIAIALFFDYTNGFHDAANAIATSVSTRALTPRVAL
                     AMAAVMNMVGAFLGTKVAATVGKGIIDPPTGTHGLVVLFAALMGAIAWNLLTWYFGLP
                     SSSSHALIGGMIGAALASSGVVFWAGVVDKVVIPMILSPTVGFLGAYLLMIGLLWAFR
                     HAAPNRVSRGFRHSQTVSAAAMALGHGLQDAQKTMGIIVLALVVTGHQTGFEIPWWVI
                     VLAASAISLGTWSGGWRIMRTLGRRVIELDPPRGFAAEATASGVLYTMAIGFGAPIST
                     THTITSAIMGVGATKRLSAVRWGVAGNIITAWVLTIPAAAAVAAAVYGVLRLFGLTG"
BASE COUNT         1135 a         2941 c         3058 g         1272 t
ORIGIN      
        1 atccggcgat cattcagcag ccggctggcc accgtgctgg tgatcgcctt gacggtgttg
       61 tggaccatcc cgacgttcgg gctgctcgtc acgtcattcc gtccgcagtc cgaggtggcc
      121 acgagcgggt ggtggacatt cttcaccaac ccggaggtca ctctcggcaa ctactacacg
      181 gtgctcatca gcggcggctt caacgtgccc ggcgggttaa tgcccttctt tgtcaactcc
      241 ttcgtcatcg cgatcccggc cacgctgatc ccgctcacga tcgcggcgat ggcggcctac
      301 gcgctggcct gggtcaactt ccgcggcagc gacatcctct tcttcacgat cttcgccctg
      361 caggtggtgc cgctgcagat ggcgctgctg ccgttgctgc agttcttcaa cgacgggttc
      421 tcgatcgggt cggtaacgct gttcccggca ctgcacttgt cggggtcgtt cttcactgtg
      481 tggatcgcgc acacgatgtt cgcgattccg ctggcggtgt tcctgctgca caacttcatg
      541 gcgcagcttc cgcgcgacat catggaagcc gcccgggtcg acggcgccag cttcttcatg
      601 agcttccgca agatcgcgct gccactgtcg gtgccggcgc tcgccgcctt cgcgatcttc
      661 cagtttctct gggtgtggaa cgacctgctg gtcgcgctga ccttcgtcgg cggccagccc
      721 acggtggcgc cgctgaccgc gcggctctcg cagctgaccg ggtcgctcgg cagccgctgg
      781 gacctgctca ctgctggcgc tttcatctcg atcgtggtgc cgctcatcgt gttcttcgcg
      841 ctgcagcgct acttcgtccg cgggctgctc gccggctccg tcaagggctg atcaccgacc
      901 agcgggtcaa ggggcggcgt cgtgcagcag ggccaacgtc tccgccaggt cggcgtcgaa
      961 ggccactgcg ggttggccga gtgccagggc gttgagggct actgcctcta gccggtgcac
     1021 ccgcccgtcg aggtaagccg cgcacgcggc tcgcgcctcc tgcagcgcgt ggtgccaggg
     1081 cagcaccccg acccgggtca ccgccccgat cgactcatgc agcactggca gctgcttggc
     1141 cgcggcctgc tcgaccagct cccgtagccc ggcgcgaacg tcagccggcg cggtctgcag
     1201 ctgcggtgag gcgtattcca cgatcgaggc gactgaccgc tcggcatagg cggtgtgccc
     1261 ctgcgccgtc gtccaggccc gcagcagcgc gtcgtactcc cgctcggcca ccacgccgtc
     1321 tgcccagcgc agccccaacg cagccgccac cagcacgacc agcagcaccc agggccagcg
     1381 ccgccggcga cttcgcggcg ggtcgccccc tcgcagcacg tcgagcccgg ccaccggacc
     1441 aacgcctctg actcagccgg ccggggcgcc cgcgcggatc aggtcggcgt accaggcgaa
     1501 tgaccgcttc ggcacccgcg ccagcgtgcc cggctcgacc gcgaccagcc cgaaccgttg
     1561 cgtgtagccg tggctccact cgaagttgtc cagcagcgtc caggccagat aggcccgtac
     1621 gtcggctccg gctgcgcggg ccgcgtgcac ggcggcgatg tgcgcctgca ggtaggccgc
     1681 ccggtcggcg tcgtcgatgc gaccgtctgg cccgatgacg ccgtcgccgt acgccgcgcc
     1741 gttctcggtc acccgcagca ccagccgtgg gtcgcgcgcc gccagctcga ccagcacgtc
     1801 ggtcagcccg ccggggtcga cctcccagcc catgggggtg cgcggctcgc gggggcgaaa
     1861 gcgcaggggt agggcgccgg ggtaggcggc cacctcgggg tcggcgtcgg agtcgtcggc
     1921 gccgggctcc ggcgagccga ctcgcagcgg gttgtagtag ttgaccccga tccagtcggc
     1981 cgacccggcc accagagtca ggtcgcccgc gtcgaccacc tcggggtcct ccagcaccgg
     2041 caccgcctcg agcaggtcgg cggggtagcg cccgtcgagc agcgggtcga gccagagccg
     2101 gttgcgtacg gcgtcgacga agcgggccgc ctcgaccgcg cgcaggtcgt cggtgtgcac
     2161 ggtggtgagg ttgagcgcca ggccgacctt cgactcggcg tgctgctcgc gcatcgtcgc
     2221 cgcggccagc gcgtgggcca gcagcaggtt gtgcgcggcg gcgtaggccg cgcccggctc
     2281 gcgccggccc ggggcgtgaa ccccggcggc atagccgagg aaggccgagc accacggctc
     2341 gttcagcgtc gcccacactc cgacccggtc gctcaggcgg ccgtggaccg ctgcggcgta
     2401 gtcggcgaac cgcagcgcgg tctcgcgcga gggccagccg ccgtcgtcct ccagcgactg
     2461 gggcaggtcc cagtggtaga gcgtcggcaa cggctggatc cccttggcac acaacgcatc
     2521 cacgactcgg tcgtagtagt ccaggcctgg ggtgttgatc ggtccgctgc cttcgggctg
     2581 cacccgtggc caggcgatgg agaagcggta ggcgccgacg cccagctcgg cgagcaggtc
     2641 gaggtcgtcg gcccaccgct cgtaggagcc gcacgcggtg cgcccgtccg agccgtcgac
     2701 gatcgcgccg ggttgggcgg cgaaggtgtc ccagatcgag gggccccgcc cgtccgcgtc
     2761 ggtcgcgccc tccacctgga aggccgaagt cgcgacgccg tacacgaacg ggcccatggg
     2821 gctcaggcta ggcgtgcgtc ggcgctagcg ctgggggctc gggtggttgg gctcccagtc
     2881 gtagatcacc gaggtgcgta cgacgaggtc ctgcaccgac ttgttgcgcg ggctgaccac
     2941 gacccagaac agcccgatcg ggaacagcac gcacatgccg gcacgcagca gcgccaccgg
     3001 caggctcggc cgagtccccc tccagttgat gacgcgcagc cccatcacgt ggctgccgta
     3061 ggagcgtccg gtcacccacc agcaccccgc cagatagagc gcgagcgttc ccagcccgac
     3121 ccccagcacc agcccgaagg aggggcgcgg gaagcgaaag ccggtcgggt cggcgacgaa
     3181 ggccagcccc gaccaggcca ggtagagcgc ggccacgatc agggaggcga cgacggcgtc
     3241 gatgacgctg cccagcaggc gggtcacgat gcccgcatgt cgaccttggt agacccgagc
     3301 ctcccggggg actggtgtga atcggccggt cacgactcct cggtcgattc gggggccggc
     3361 gcctcgatcg cctggcgggc cggttcttcg atcggctgtc gaggagccgg gacgtctccg
     3421 cggtgatgac cggggtggcg ctggcgcccc atcagccggt ccatgaagcc cgacaccgcc
     3481 tcgtcggcct cgatgctctg caggcgtacg tcgcgcaccg cctccgaggc gaccgagccg
     3541 gaggacgacc ggatgatctc ggccaggtcg acgcggtcga tgacgtcctc ggccaggtcc
     3601 acgaggtcga gccgggccac gatcgcgtcg aggtcgatcc ggctggcgat cgcgtcgacg
     3661 tcgaccgagg cgatgagggc gttgaggtcg agccggcgca ggatctgctc aaggtccagg
     3721 cgcgccgcga cccggtcgat gtccaggcgt gccgcgatcc ggtcgatgtc gagccgggct
     3781 gccgcggcgt ccaggtcgag tcgcgcggcc acggcgtcga cgttcagccg agctgcggcg
     3841 gcgttgaggt cgacgtgggc gaccagcctg tcgaggtcga cgtggcgacg gaccagctcg
     3901 gtgaggtcga gccggtcgag caccgcctcg accagccgcg gcagcagcgc gtcgagcagt
     3961 cgggtcgcct cgggctcggc cagggcgagc tcggcccggc cgcgttcgcg ggtccggtcg
     4021 agcgcctgcg cggagcgctg cagcggcacc cgtagtggcc aggtgagcgg ccgcagcccg
     4081 cgccccaccg ccgccgcgac cccggtggcg atctcgatgg ccccggcggc cgcctcgaag
     4141 gcctgcgcgg ccaccccgac ggccagctcg gtgttgcggt cggcgccggg ccgcggaacg
     4201 ccccgcccgg cggccgcggc cacctcccgg gccgcccgcc gggagagccc gatcaccagg
     4261 cgggcgccct gccacagcgg cggcaccggg tgctgcgcgt ccatggctgc agtctgatcg
     4321 gtgatcagcg ggtcagcacg tgactttgcc cagcctgcag atggcccggt gggtgggcga
     4381 gcacccgtca gatcgagtgg agctggtcgc cgccggcccg cttggcgacg tacatcgcgt
     4441 cgtcggccag ggccagcagc tcggcgccgt cgcgcaggct catcgtcgtg gccgacccca
     4501 cgctgaccgt gacgggttcg ccgcgggtga tcgccgccca gttggtggtc cggatcgccg
     4561 ccagcatgcg ggaggccacg atggcgacct cgtcggggcc ggtgtcggtg agcaggacga
     4621 cgaactcgtc gccgccgagg cgggccacca cgtcggacga acgggcgtgc tcgacgagca
     4681 ccctggtgat cgcccgcagc acctcgtcgc cggtctggtg gcccagggtg tcgttgatct
     4741 gcttgaacga gtcgacgtcg agcatgatcg cggagaccgg gtagggcgag ggcggggcgg
     4801 tctcggccag ctcgtgcagc cgctgctcga gatggcggcg gttggccacc ccggtgagcg
     4861 ggtcctcgtg ggaggcgcgc tgggcggcca ggtgctcggt gcgcagccgt tcgagggtga
     4921 gctgggcctc ggcgccgcgc agggtgcgca gccgctgccg ccacacctcg cgggccatcg
     4981 tctcggcgta gtgcagcgcg acctgggcgg ccgggtcacc gctgcggtgg gcggccagcc
     5041 gggcgtgccg gtcgtgcacc gaggcgacca gcggccagtc ggcgtcgggg ggcagcaggt
     5101 cgttggcgaa gcgagccgct cgctcggcgt cgtcgagccg gccgaggtcc tcgagcgcgc
     5161 gggagaggaa gaccagcccg aaggtcgcct cggtggccag gtcgagagcg agtacggcgt
     5221 cgacggcacc gcgcaggagc tcgaccgcct ctgccggcgc tgcgctcaga gccagcgccg
     5281 aaccggcgag caggtcggcg gtgtggcgcc agcggccgcg gtcgagcggt ttggcctccc
     5341 gctgggcggc gcgcgcgtgc tcgagcgccg tacccgcatg ttggtgcaag gggccggtgt
     5401 cgccgacgcg ttcgagctcg aggccccagc gcaggtggaa ctcggcgagg ttgaggtggt
     5461 cgatggcccg ggactcgggc tgctggtcgg gggtgtcggg cagctcgagc ggagcgaggt
     5521 agtgcggcag ggcgagctcg tagagccgca cgaggtcgaa cccgatggcc agcccggtat
     5581 gggcgaagct gagcacgagc gggttggtgt caccggctcc gtcgagggcg acctgggctg
     5641 ccaccaggtc gcgcacgccc tcgtcgatgt cgccgagcag cacgtactgc gaggcgcgca
     5701 gcgagcgggc gttggagacc cagccctggt cgtcgatcgc ctcggcgacg gtgagcagcc
     5761 gctcgcagtc gctgatcgcc tgggcgtgct cgccgaggct gtggtgggcg accgcgcgca
     5821 cgtagtgcag ccgcggcacg agatcggatc gctgggcctc gacggcgtgc gcgagcagct
     5881 cgtcggcgag cgcgaccgct tcccgcggtg cacccgactg ggcgagctcc atcgcgcggg
     5941 tcacccgcac gtctaggcgg tccatcgcgg tcctgccgct ccctccgtct gacgcaacag
     6001 gttggtggcc gtcacctcaa tggtcggcgg aacgagcgcc gagcctgagc cgatctgggc
     6061 acgatcgtag gaggcttggc cgacgggacc ggaccggccg gtggataggg tcccgccgcc
     6121 gggggtgccg acgggtgagc agtcatggtc cttatggacc atcttgtgac agaacgtggt
     6181 gcaggcgggg gcgcaggggg aatgctctga cccggacttg cctgaggagg cgacgtgacg
     6241 agtccccgcg agctggccgc tgggtccagc ccctcagcgt cgaaggcacg ttcgaggacc
     6301 cggttgctgc gcgaccggac cgacatccgc ctgcaggatc cgcaggctct cgacgagatc
     6361 gatctgtacg gcgaggtggt gatcgccgcc tcggcgtgcg agggaccgct gagcctcgag
     6421 cagctcgacc gggtcctcgg cctgcgctga cccgccgggc ctacccccga aggggtcgcc
     6481 tcgggggaca gggggcggat gcgcgtgttg gtcgtgacta cccctagcat catgacggcc
     6541 gacccgctcg gtcaggtcgt catgtccccg cacaaccgga gcactccgtg cgctttcgtc
     6601 tggccccgcg tgagacgagc ttctacgaca tgttcgcgac ctcggcgaac aacctggtca
     6661 ccgccgccaa gctgctcaag gacatgctca gcgccgacgc gccgggtcgc gaggagatcg
     6721 cggtcaagat gcgcgctgcc gagcacgccg gtgacgaggc cacccatgcg atcctgcgcc
     6781 agctcaacgg caccttcatc acgccgttcg accgcgagga catctaccgg ctggccgccc
     6841 gcctcgacga ctgcgtcgac ttcatggagg cggcggtcga cctggtgctg ctctacaaga
     6901 tcgacgagct gccccacggg gtggccgacc aggtcgcggt gctcgagcgc tgcgccgagc
     6961 tgaccgccga ggcgatgccc cggctgcgct cgatgaccga cctgtcggag ttctggatcg
     7021 aggtcaaccg gctggagaac caggccgacc aggtctaccg gcgaatgctc gccgagatct
     7081 tcgacgggca gtacgacgcc ctgacggtgc tcaagtttaa ggaggtcatc gaccagctcg
     7141 agagcgcggc cgacgccttc gagaacgtcg ccaacatgat cgagaccatc gcggtcaagg
     7201 agtcctgacc ggtggagctg ctcgggctga tcgcgttcat cgcgatcgct ctgttcttcg
     7261 actacaccaa cggcttccac gacgcggcca acgcgatcgc caccagcgtc tccacccggg
     7321 cgctgacccc gcgggtggcg ctggccatgg ccgcggtgat gaacatggtc ggggccttcc
     7381 tcggcaccaa ggtggcggcc accgtcggca aagggatcat cgatccgccg actggcaccc
     7441 acgggctcgt cgtactcttc gccgctctca tgggtgcgat cgcctggaac ctgctgacct
     7501 ggtacttcgg gctgccctcc tcgtcgtcgc acgcgctcat cggcggcatg atcggcgcgg
     7561 cgctggcctc gtcgggcgtg gtgttctggg ctggcgtggt cgacaaggtc gtcatcccga
     7621 tgatcctttc gccgaccgtc gggttcctcg gcgcctacct gctgatgatc gggctgctgt
     7681 gggcgttccg acacgccgcg cccaaccgcg tcagtcgcgg cttccgccac tcgcagaccg
     7741 tctcggccgc cgcgatggcg ctgggccacg gcctgcagga cgcccagaag acgatgggca
     7801 tcatcgtgct agccctggtg gtgaccgggc accagaccgg gtttgagatc ccctggtggg
     7861 tcatcgtcct cgctgcctcg gcgatctcgc tgggcacctg gtcgggcggt tggcggatca
     7921 tgcgcaccct gggtcggcgg gtgatcgagc tcgacccacc acgcgggttc gccgccgagg
     7981 ccacggcttc cggagtgctc tacacgatgg cgatcgggtt cggcgcgccg atctcgacca
     8041 cccacaccat cacctcggcg atcatgggtg tgggcgcgac caagcggctc tcggcggtgc
     8101 gctggggcgt ggccggcaac atcatcaccg cctgggtgct cacgatcccg gcggccgcag
     8161 cggtggcggc ggcggtctac ggcgtgctga ggctgttcgg tctcaccggc tgacgccgcc
     8221 gcgagtgctg acggtgtgca cggcaatccg tgccgcagcc cggcgctcga ggtgctgttt
     8281 cggacgtacg gcgactagat cagcggtccg ccgtcgcggg ggcgtcggcc ggtgggagtt
     8341 gttgtctgct aggtgccctc cgcggtggag tcaggcccgt ggctgcttgt gccgagcgcc
     8401 gtaccc
//
LOCUS       DATIBB010000081         3060 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_961533, whole genome shotgun sequence.
ACCESSION   DATIBB010000081 DATIBB010000000
VERSION     DATIBB010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3060
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_961533"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..582)
                     /locus_tag="VLC50_02890"
     CDS             complement(<1..582)
                     /locus_tag="VLC50_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006133870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="HSF26443.1"
                     /translation="MAKIIAFDEEARRGLERGMNQLADAVKVTLGPKGRNVVLEKKWG
                     APTITNDGVSIAKEIELEDPYEKIGAELVKEVAKKTDDVAGDGTTTATVLAQALVKEG
                     LRNVAAGANPMALKRGIEKATAAVSEQLLEMAKDVETKEQIASTASISAADTQIGEMI
                     AEAMDKVGKEGVITVEESNTFGLELELTEGMRFD"
     gene            complement(805..1011)
                     /locus_tag="VLC50_02895"
     CDS             complement(805..1011)
                     /locus_tag="VLC50_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013732668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HSF26444.1"
                     /translation="MAQGSVKWFNAEKGFGFIEVDGGEPDVFVHHSAIQMDGYRTLEE
                     KQRVEFEVAPGRDGRMQAANVRAI"
     gene            1314..1472
                     /locus_tag="VLC50_02900"
     CDS             1314..1472
                     /locus_tag="VLC50_02900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF26445.1"
                     /translation="MVAAYLLAAGWSWLRCGDPASYNHFERLAGLADGGYPPPGERAG
                     AVRRGRPG"
     gene            1483..2943
                     /locus_tag="VLC50_02905"
     CDS             1483..2943
                     /locus_tag="VLC50_02905"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018958812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HSF26446.1"
                     /translation="MAEAPLVLASNRGPVSFTEADDGAPRAKRGGGGLVSALGSIHGS
                     GALWVCAALSDADRRVARAAPGGRLDLAGHETGTGAVRMLPIDPSTLARAYNGIANST
                     LWFVHHLLYAIPTAPSFDATWRREWAAYRAYNEAFADAVAEEVAPGGRVLVQDYHLAL
                     APAMLRARRPDLRIGHFSHTPWAPPDYFRVLPDDVARAVLEGVLGADRAGFLSPRWAR
                     AFLDCAAAVLGARVDPEAMTVEHDGRRTQVGVHALGVDAADLRARADQPDVRARIAAL
                     RDRVGDRLVITRIDRTELSKNIVRGLVAYRELLRTHPEWQGRVTHLAFAYPSRHDIPE
                     YREYTAAVQRVATEIEEEFGTDDWSPLVLAVDDDYARSLAAYRIADVLLVNPVRDGMN
                     LVAKEGPILADHGAAVVLSTEAGAADEMAEDAFLINPYDVCATAQALHEALSLPPDQR
                     RARSDRLAAVAAALPPQRWLAEQVDALDGPPVSHPA"
BASE COUNT          387 a         1122 c         1080 g          471 t
ORIGIN      
        1 gtcgaagcgc atgccctcgg tgagctcgag ctcgaggccg aaggtgttgc tctcctcgac
       61 ggtgatgacg ccttccttgc cgaccttgtc catcgcctcg gcgatcatct cgccgatctg
      121 ggtgtcggcg gccgagatcg aggccgtgga ggcgatctgc tccttggtct cgacgtcctt
      181 ggccatctcg agcagctgct cgctcacggc cgcggtggcc ttctcgatgc cccgcttgag
      241 ggccatcggg ttggcgccgg cggcgacgtt gcgcaggccc tccttgacca gggcctgggc
      301 cagcacggtg gcggtggtgg tgccgtcgcc cgcgacgtcg tcagtcttct tggcgacctc
      361 cttgacgagc tcggcgccaa tcttctcgta ggggtcctcg agctcgatct ccttggcgat
      421 ggacacacca tcgttggtga tcgtgggggc gccccacttc ttctcgagca cgacgttgcg
      481 gcccttgggg cccagcgtca cctttacggc gtcggcgagc tggttcatgc cccgctcgag
      541 gccgcggcgc gcctcctcgt cgaacgcgat gatcttggcc atgcggttcc ggtcctcccg
      601 gtacggggtg gatggcgagc ctggtacgac gcccgcgacg gctggcccgg cgccggtggc
      661 gatccgtctt gccaccggca gtgcccggac ctcgacgccc cggctcgaac tgctgtcact
      721 cgacggtgcc gagtgctaac gcaatgatta gcactcgacc ctgtcgagtg caagcccgtt
      781 gggagtaggg aacccggcga cctgctagat cgcgcgcacg ttggcggcct gcatccggcc
      841 gtcacggccg ggggccacct cgaactccac ccgctgcttc tcctccagcg tgcggtagcc
      901 gtccatctgg atggcgctgt ggtgcacgaa gacgtccggc tcgccaccgt cgacctcgat
      961 gaagccgaac cccttctcgg cgttgaacca cttgacgctg ccctgggcca ttccggttgc
     1021 tcccctgtac gtgtgccgaa ctgccgacgg ccgcagagag tagccgtcag gtggctctgt
     1081 gaacaccaca gtgatcacac ggccaccgct tcgcagccga gtcgttaggt tcgcgacgta
     1141 cgcctcggac tgcggctacg ccgggtggtc aacctggtga ccctcgccac cccggccggg
     1201 ctggctctcg cccggctcgg cggggcacgt ccgcggccgg gtccgggcgg gctggtgctc
     1261 tgcgccacga ggcccgccac gccacccagt acgcctgctg cgccggtctg ccgatggtgg
     1321 cggcctacct gctggccgcc ggctggtcgt ggctgcgctg cggtgacccc gcgtcgtaca
     1381 accacttcga acgactggcc gggcttgccg acggcggcta cccgccgccc ggagagcgcg
     1441 caggcgccgt acggcgaggc cggcctggct agggtcgctg ccatggccga agccccgctc
     1501 gtgctcgcgt ccaaccgcgg accggtctcc ttcacggagg ccgacgacgg ggcgccgagg
     1561 gccaagcgcg ggggcggcgg gctggtctcg gcgctgggca gcatccacgg ctcgggggcg
     1621 ctgtgggtgt gcgcggcgct gtccgacgcc gaccggcggg tcgcccgcgc cgccccgggc
     1681 gggcgcctcg acctggccgg gcacgagacc ggcaccggcg ccgtacggat gctgccgatc
     1741 gacccgagca cgctggcccg ggcctacaac gggatcgcca actcgacgct gtggttcgtc
     1801 caccaccttc tctatgcgat cccgaccgca ccgagcttcg atgcgacctg gcggcgggag
     1861 tgggcggcct accgggccta caacgaggcg ttcgccgacg cggtggccga ggaggtggcc
     1921 ccgggcgggc gggtgctcgt gcaggactac cacctagcgc tggccccggc gatgctgcgg
     1981 gcgcgccggc ccgacctgcg gatcgggcac ttcagccaca ccccgtgggc cccgcccgac
     2041 tacttccggg tgctgcccga cgacgtggcg cgggcagtgc tcgagggcgt cctgggcgcc
     2101 gaccgcgccg gcttcctctc cccgcggtgg gcccgcgcgt tcctcgactg cgcggccgcg
     2161 gtgctcggcg cccgggtcga ccccgaggcg atgacggtcg agcacgacgg tcggaggacg
     2221 caggtcgggg tgcacgcgct cggggtcgac gccgccgacc tgcgcgcgcg cgccgaccag
     2281 cccgacgtac gggcccggat cgccgccctg cgcgaccggg tcggcgaccg gctggtgatc
     2341 acccgcatcg accgcaccga gctgagcaag aacatcgtgc gggggctggt cgcctaccgc
     2401 gagctgttgc gcacccatcc cgagtggcag ggccgggtca cccacctggc cttcgcctac
     2461 cccagccggc acgacatccc ggagtaccgc gagtacaccg cggcggtgca gcgggtggcg
     2521 accgagatcg aggaggagtt cggcaccgac gactggtcgc ccctcgtgct ggccgtcgac
     2581 gacgactacg cccgctcgct ggccgcctac cggatcgccg acgtcctgct ggtcaacccg
     2641 gtccgcgacg ggatgaacct cgtggccaag gagggcccga tcctggccga tcacggcgcc
     2701 gcggtggtgc tctcgaccga ggccggtgcc gccgacgaga tggccgagga cgcgttcctc
     2761 atcaacccgt acgacgtgtg cgcgaccgcg caggcgctgc acgaggcgct gtcgctgccg
     2821 ccggaccagc ggcgcgcccg cagcgaccgg cttgccgcgg tggccgccgc gctgccgccg
     2881 cagcgatggc tggccgagca ggtcgacgcg ctcgacggcc cgccggtcag tcacccggcg
     2941 tagtcggttg ggccgatggg gcgacgcccg cccacaccgg gatgacactg tcagtaaccg
     3001 tcgctagcct acttttgtgc ttcacatcgg gggaaaaacc acaaagggct actccggccc
//
LOCUS       DATIBB010000082        12372 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_831998, whole genome shotgun sequence.
ACCESSION   DATIBB010000082 DATIBB010000000
VERSION     DATIBB010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 12372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12372
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_831998"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(139..1161)
                     /locus_tag="VLC50_02910"
     CDS             complement(139..1161)
                     /locus_tag="VLC50_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HSF26447.1"
                     /translation="MAAGQEVDVGSNAPFGLAGVDPVLEASLRARLAEVESLLRASVT
                     SDYPFVTETSRHLVDAGGKRFRPLLVLLAAQFGDPDAPGVLPAAVVVELTHLATLYHD
                     DVMDEAALRRGAPSANSRWDNTVAILTGDFLFARASDLLADLGPEAVRIQARTFERLV
                     TGQIRETVGPAPDEDPISHHLSVLADKTGSLIATSGRFGALLAGAAPEVVSTLTRYGE
                     NIGVAFQLADDLLDVASESGQSGKTPGTDLREGVATLPVLLARREAGQRDARLLELLD
                     GDLRDDHRHAEALRLLRGHPAMEAARQELDRWTEAARACLDPLPEGAARDALVAMCDL
                     VGNRVG"
     gene            complement(1213..2760)
                     /gene="nuoN"
                     /locus_tag="VLC50_02915"
     CDS             complement(1213..2760)
                     /gene="nuoN"
                     /locus_tag="VLC50_02915"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018558804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HSF26448.1"
                     /translation="MSPLAAVIEAPTIEYSKVAPLLIVFGVAVVGVLVEAFAPRSRRY
                     VIQLVLALAGLAASLAAVIGLAGTHAIVAMGSVAIDGPTLFLQGTILVLAMLAVLTMA
                     ERALDPGGTAFAAQVSALPGTEAERTATSTQVMQTEVFPLTMFSVAGMLLFPAANDLL
                     TMFVALEVLSLPLYLLAGMARRRRLLSQEAALKYFLLGAFSSAFFLYGIAMLYGYSGT
                     VTLAGIADAVSGSAGNDALLLLGTALVTVGLLFKLGGAPFHSWIPDVYHGAPTPITGF
                     MATATKVAAFGALLRVLYVGLGGMRWDWRPMMWVIAVLTMAVGCVVAITQTDIKRMLA
                     YSSIAHAGFVLTGVMGTNRDGISSVLFYLLAYGFSTIGAFAVVTLVRNAGGEATHLSQ
                     WAGLGRRSPLVAGVFTLFLLAFAGIPLTSGFTAKFAVFQAALSGGETILVVIAVLASA
                     VAAFFYVRVIVLMFFNEPASEGTSVVLPSPLTTIAITVGVAMTVVLGVLPGWFFDLAD
                     QAAIFAR"
     gene            complement(2757..4286)
                     /locus_tag="VLC50_02920"
     CDS             complement(2757..4286)
                     /locus_tag="VLC50_02920"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020578382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HSF26449.1"
                     /translation="MTDFPWLTVLGAVPLVGAVGVALLPRGRDLLAKQVALAVSLVAL
                     VLTLAVALRFDPDVSGFQLTETHAWIPQFGISYAVGVDGIALVLLGLTAILTPICILA
                     AWHDADAGKRSVKAYFALILVLETMCFGVFSATDVFLFYFFFEAMLVPMYFIIGSYGG
                     AQRSYAAVKFLLYSLLGGMLMLAALIGLYVVSPGGDQAFLLDNLIGMPIDATTGKWLF
                     LGFFAAFAVKAPMWPVHTWLPDAASEAPPSGAVLLVGVLDKVGTFGMIRLCLPLFPDA
                     SKWATPAIIVLATVGVIYGALLAIGQRDVMRLIAYTSISHFGIMVLGIFAMTSQGQAG
                     ATLYMLNHGLVTGALFLIAGMMIHRRGSRMIADFGGVQRVAPMIAGSFLVAGLAGLAL
                     PGLNSFVSEFLSLVGTFTRYPAAAIVATTAIILAAIYILLTYQRMCTGPVTEHTKSWT
                     DMTRREVWVVAPLIAVIVALGFYPKPVLDVITPAVQTTMTQVGVQDPAPTVPVAEGTQ
                     Q"
     gene            complement(4288..6210)
                     /gene="nuoL"
                     /locus_tag="VLC50_02925"
     CDS             complement(4288..6210)
                     /gene="nuoL"
                     /locus_tag="VLC50_02925"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006590665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HSF26450.1"
                     /translation="MHAAAALETAQASGAFSLTWLLVALPLAGAAVLLVGGRRTNPVG
                     HIVGTAASWASFVVAAILFVAMLGRDSDARAVTQHLFSWIPVEGFQVDASLLVDPLSL
                     AFVLLVTFVGSLIHVYSIGYMADDPDRRRFFAYLNLFVAAMLLLVLSDGYLTLYVGWE
                     GVGLASYLLIGFWNHNPTYAAAAKKAFVVNRVGDVGLSIAIMIMFATFGRTDFAGVFA
                     GAEGASEGILTAIGLMLLLAACGKSAQFPLQSWLGDAMAGPTPVSALIHAATMVTAGV
                     YLIVRSAPIFDGAPTAQLLVVIVGVITLLFGAIIGCAKDDLKKALAASTMSQIGYMML
                     AAGLGPVGAAFAIFHLLTHGFFKAGLFLGAGSVMHGMNNRIDMRHFGGLSAVMKITWV
                     TFGLGWLSIIGFPLLSGFWSKDKIIEAAFIGEGWRPWVFGGAALLGAGITAFYMTRLF
                     AMTFHGERRWDEDVHPHESPLVMTLPMMVLALGSAFLGVILGPTGWITSWLEPVTGSH
                     GEAEPVISEPVIIGVTLVLVAVGAGLAWVTYAARSVPRTVPAAGWLTVAARRDLYQDA
                     FNEATFMRPGQYLTRSLVFFDNRGVDGLVNGLAALVGGSSGRLRRLQTGFVRSYALSM
                     FAGAALVVAALVLVRL"
     gene            complement(6230..6529)
                     /gene="nuoK"
                     /locus_tag="VLC50_02930"
     CDS             complement(6230..6529)
                     /gene="nuoK"
                     /locus_tag="VLC50_02930"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019971632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HSF26451.1"
                     /translation="MSPVNYLYLSALLFSIGGAGVLLRRNAIVVFMCIELMLNAANLA
                     FVTFARMQGNLDGQIIAFFVMVVAAAEVVVGLAIIITIFRTRRSASVDDANLLKY"
     gene            complement(6526..7326)
                     /locus_tag="VLC50_02935"
     CDS             complement(6526..7326)
                     /locus_tag="VLC50_02935"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020135933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HSF26452.1"
                     /translation="MASAPTVLAAAQTGAGEAWLFWICAPLSVLAALGLVMAKKAVHS
                     ALMLALIMICLALFYIAQQAPFLGIVQVVVYTGAVMMLFLFVLMLVGVDSSDSLVETL
                     RGQRVAGLLAAVAFALLLVGGIGRAVFADGTGLTQANAEGNVPAVAEMLFTRYVWGFE
                     VTSALLITAALGTMVMAHRERLARRLTQRELAEARFRSGGLVTMLPAPGVYARHNAVD
                     VPGLLPDGRPSELTINRVLAARGDVRDIHEFDTRPVTEAITDSSEDAQ"
     gene            complement(7326..7910)
                     /gene="nuoI"
                     /locus_tag="VLC50_02940"
     CDS             complement(7326..7910)
                     /gene="nuoI"
                     /locus_tag="VLC50_02940"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018538816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HSF26453.1"
                     /translation="MPKFLDPIAGFGVTFSTMFRKVVTEQYPEEKKQTAPRFHGRHVL
                     NRHPDGLEKCIGCELCAWACPADAIYVEAADNTDEERFSPGERYGRVYQINYLRCIFC
                     AMCVEACPTRALTMTNEFELADSSRESLIYEKHDLLAPLLPGMVAPPHAMAPGTTDKD
                     YYHGRVTRAVPEQGPVLTPGDGTPDSAAAGEARP"
     gene            complement(7903..9276)
                     /gene="nuoH"
                     /locus_tag="VLC50_02945"
     CDS             complement(7903..9276)
                     /gene="nuoH"
                     /locus_tag="VLC50_02945"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010986265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HSF26454.1"
                     /translation="MSRHLLAAESAPATGLFGTDPWWLVLLKAVAIFVFLVVMTLFAI
                     WWERRVVARMQHRIGPNRLGPQGLLQSLADGIKLALKEDLIPKTADKVVFILAPVLAA
                     VPAFMAYAVMPFGPKVSIFGHSTMLQLTDMPIAVLYVIAVTSVGVYGIVLAGWSSGST
                     YPLLGGLRSSAQVISYEVAMGLSFVGVFLFAGSMSTSQIVDAQTSLWFALPLIPSFLI
                     YMTAMVGETNRAPFDLPEAEGELVGGFHTEYSSLKFALFFLAEYINMVTVSTLATTLF
                     LGGWRAPWPLSAIGVDELHPTGLLNTGWWPLLWFLLKINLFIFVFIWLRGTLPRLRYD
                     QFMKLGWKLLIPLSLAWILAVAAIRAISNEPTFDRRALFIWVGGGLAVVFLATMAWDA
                     WAARRAGEPEESTEPVEFDPMAGGYPVPPMPGQVLPAYATASARRSVRDASPDGPTSE
                     RQEVTDA"
     gene            complement(9273..11711)
                     /locus_tag="VLC50_02950"
     CDS             complement(9273..11711)
                     /locus_tag="VLC50_02950"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF005895.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit G"
                     /protein_id="HSF26455.1"
                     /translation="MTVTSTPGAGAQQGEDLVTLTIDGVVVSVPKGTLVIRAAEMIGT
                     QIPRFCDHPLLDPVGACRQCLVEVAGQRKPLASCTTTVSEGMVVKTQLTSPVAEKAQY
                     GVMELLLINHPLDCPVCDKGGECPLQNQAMSNGRAESRFEGVKRTYPKPIAISSQVLL
                     DRERCILCARCTRFSDQIAGDPFIDMVERGALQQVGIYEKEPFDSYFSGNTVQICPVG
                     ALTGAAYRFRSRPFDLVSTPSICEHCASGCAIRTDHRRGKVLRRMAGDDPAVNEEWNC
                     DKGRWAFQYATMPERITTPLVRDEESGELVPTSWPDALAVAAAGLRAARDGAGVGVLP
                     GGRVTLEDGYAWSKFARLALGTNDVDFRARPHSAEEAAFLGHAIVGTSPATGGVTYAD
                     LEHAPAVLLVGFEPEEESPMVFLRLRKGMRKKSVPVFSVAPYATRGLHKMGGRLLPTA
                     PGTEPEVLAAITAADEAVAAVAEAVRRPGAIVLVGERAASVAGTLSAVLALVAETGAR
                     LAWVPRRAGDRGALEAGLLPGLLPGGRPVDDAPARVDVGTVWGVSVPAQPGRDATTML
                     TAARDGALGGLVVGGVDPDDLPDPVLALEALDRVPFLVSLEQRVSAVTSRADVVLPVA
                     PVVEKSGTFLDWEGRERVFGTVLSTSAMPDLRVLSVLADELDAPIGVRDVAEARRELA
                     ELAAWDGARVPAPSVEAGPPPEPGPGQAVLATWAQLLDLGTLQAGEPFLAGTAHASAV
                     RLSAATAAEIGAADGDKVTVATAAGELTLPLTITAMPDRVVWVPTNSVGSQVRPTLAV
                     DTGAVVTITAGSAA"
     gene            complement(11708..>12372)
                     /locus_tag="VLC50_02955"
     CDS             complement(11708..>12372)
                     /locus_tag="VLC50_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020578389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HSF26456.1"
                     /translation="NGPSWFAGMGTEKSKGMTIYSLSGHVTRPGQYEAAMGCTLRELL
                     ELAGGVREGHELKFWTPGGSSTPILTADHLDVPLDYEGVGGAGSMLGTKALQIFDETT
                     CVVRAVRRWSAFYAHESCGKCTPCREGTYWYVQIFDRLEAGNGTEEDLEKLLDLSDNI
                     LGRAFCALGDGATSSVTSSIQYFRDEYIAHFTHGGCPFDPAASTVFAAASSTSSTGAS
                     AS"
BASE COUNT         2082 a         4232 c         4495 g         1563 t
ORIGIN      
        1 ggtttggccg gtgaaccaac accgcacgtc ttatgttagt ctgacattac agatggtagg
       61 tggcgggctg aggccacgtc aagcgacgcc acttccccag gggtggtcga ggccgcggcc
      121 ggcgcggttg cccaggcctt agccgacgcg gttgccgacc aggtcgcaca tggcgaccag
      181 cgcgtcacga gcggccccct cgggcagcgg gtcgaggcag gcccgagccg cctccgtcca
      241 ccggtcgagc tcctgacggg cggcctccat cgccggatgc ccgcgcagca gccgcagcgc
      301 ctcggcgtga cggtggtcat cacgcaggtc gccgtcgagc agctcgagca gccgcgcgtc
      361 gcgttggccg gcttcacggc gggccagcag caccggcagg gtcgcgactc cctcgcgcag
      421 gtcggtgccc ggggtcttgc ccgactgccc ggactcgctg gccacgtcga gcaggtcgtc
      481 ggcgagctgg aacgccaccc cgatgttctc gccatagcgg gtcagcgtcg acaccacctc
      541 cggcgcggcg ccggcgagca gggcgccgaa gcgcccagag gtcgcgatga gcgaaccggt
      601 cttgtccgcc agcacggaca ggtggtggct gatcgggtcc tcgtcagggg ccgggccgac
      661 cgtctcacgg atctgaccgg tcaccagccg ctcgaacgtg cgggcctgga tgcgcacggc
      721 ctctggcccg aggtcggcca acaggtcgga ggcccgggcg aagaggaagt cgccggtgag
      781 gatggcgacc gtgttgtccc accgggagtt ggcgctgggc gcgccgcggc gcagcgccgc
      841 ctcgtccatc acgtcgtcgt ggtagagcgt cgccaggtgg gtcagctcga cgacgacggc
      901 cgcgggcagc acgccggggg cgtcgggatc gccgaactgg gccgcgagca gcacgagcag
      961 cggacggaac cgtttcccgc cggcgtcgac caggtgccgt gacgtctcgg tgacgaaggg
     1021 gtagtcactg gtgaccgagg cacgcagcag cgactcgacc tcggccagcc gggcacgcag
     1081 cgacgcctcg agcaccgggt cgacgcccgc aagcccgaac ggggcgttgc tgcccacgtc
     1141 gacctcctgc cctgctgcca cgggcgctga cgctaccgcg tcgtacgccg tccctcacat
     1201 ccgcggacac gctcagcgcg cgaagatcgc cgcctggtca gccaggtcga agaaccaccc
     1261 gggcagcacg ccgagcacca cggtcatggc cacgccgacg gtgatcgcga tggtggtcaa
     1321 cgggctgggc aggaccacgc tggtgccctc gctggccggc tcgttgaaga acatcagcac
     1381 gatcacccgc acgtagaaga atgcggcgac cgcgctcgcc agcacggcga tgaccacgag
     1441 gatcgtctcg ccaccggaca gcgccgcctg gaagaccgcg aacttggcgg tgaacccgct
     1501 ggtcaacggg atgccggcga aggcgagcaa gaacagcgtg aacacccctg cgaccaacgg
     1561 ggaacggcga ccgagcccgg cccactgtga gaggtgggtc gcctcaccgc cggcgttgcg
     1621 caccagggtg accaccgcaa atgcgccgat cgtcgagaag ccgtaggcga gcaggtagaa
     1681 cagcacgctc gagatgccgt cgcggttggt tcccatcacg cccgtgagca cgaacccagc
     1741 atgcgcgatc gaggagtagg ccagcatccg cttgatgtcg gtctgggtga tcgccaccac
     1801 acacccgacc gccatggtca ggaccgcgat cacccacatc atgggccgcc agtcccaccg
     1861 catcccgccg agaccgacgt agagcacccg aagcagcgcc ccgaacgcag cgaccttggt
     1921 cgccgtggcc atgaagccgg tgatcggggt gggggcgccg tggtagacgt cggggatcca
     1981 gctgtggaac ggcgcgccac cgagcttgaa cagcagcccg acggtgacca gcgcggtgcc
     2041 cagcagcagc aacgcgtcgt tgcctgccga ccccgacacc gcgtcggcga tgccggccag
     2101 ggtcaccgtg ccggagtagc cgtagagcat ggcgatgccg tagaggaaga acgccgagga
     2161 gaacgcgccg agcaggaagt acttcagcgc cgcttcctgg gagagcagcc ggcgacgacg
     2221 ggccatgccg gccagcaggt agagcggcag cgacagcacc tcgagcgcga cgaacatcgt
     2281 cagcaggtcg ttggccgccg ggaagagcag catgcccgcg acggagaaca tggtcagcgg
     2341 gaagacctcg gtctgcatga cctgggtgct cgtcgcggtc cgctcggcct cggtgccggg
     2401 cagcgcagag acctgcgcgg cgaaggccgt gccgcccggg tcgagcgcgc gctcggccat
     2461 cgtgagcacg gcgagcatgg ccagcacgag gatcgtgccc tgcaggaaca gcgtgggccc
     2521 gtcgatggcg accgagccca tcgcaacgat ggcgtgggtg ccggccaggc cgatcaccgc
     2581 ggccaggctg gcggccagcc cggccagcgc cagcacgagc tggatcacgt agcggcggga
     2641 gcggggcgcg aacgcctcga ccagcacgcc gaccaccgcg acgccgaaca cgatcagcag
     2701 cggcgcgacc ttggagtact cgatcgtcgg tgcctcgatc acggcggcga gcgggctcac
     2761 tgctgggtcc cttcagcgac ggggactgta ggggccgggt cctgcacgcc gacctgcgtc
     2821 atggtcgtct gcacggcggg ggtgatgacg tcgagcaccg gcttgggata gaagccgagg
     2881 gcgacgatga ccgcgatcag cggggcgacc acccacacct cgcgtcgggt catgtcggtc
     2941 cacgacttgg tgtgctcggt gaccggcccg gtgcacatcc gctggtaggt cagcaggatg
     3001 tagatcgccg ccaggatgat cgccgtggtc gcgacgatcg ccgcggccgg atagcgggtg
     3061 aacgtgccga ccagcgagag gaactcgctg acgaagctgt tgagcccggg cagggccaac
     3121 ccggccagcc cggccacgag gaacgacccc gcgatcatcg gggcgacccg ctgcaccccg
     3181 ccgaagtcgg cgatcatccg tgagccccgg cggtggatca tcatgccggc gatgaggaag
     3241 agcgccccgg tgacgaggcc gtggttgagc atgtagagcg tggccccggc ctggccctgc
     3301 gaggtcatcg cgaagatgcc gagcaccatg atcccgaagt gcgagatcga cgtgtaggcg
     3361 atcagtcgca tcacgtcgcg ctggccgatg gccagcagcg cgccgtagat caccccgacc
     3421 gtggccagca cgatgatcgc cggggtcgcc cacttggagg cgtcggggaa cagcggcagg
     3481 cagagccgga tcatgccgaa cgtgccgacc ttgtcgagca caccgacgag gagtacggcg
     3541 cccgagggcg gcgcctcgct cgccgcgtcg ggcagccagg tgtgcaccgg ccacatcggc
     3601 gccttgaccg cgaacgccgc gaagaagccg aggaacagcc acttgccggt cgttgcgtcg
     3661 atcggcatgc cgatcaggtt gtcgagcagg aaggcctggt cgccaccggg cgagacgacg
     3721 tagaggccga tcagcgcggc gagcatgagc atgccgccga gcagcgagta gagcaggaac
     3781 ttcaccgcgg cgtaggagcg ctgggccccg ccgtaggagc cgatgatgaa gtacatcggc
     3841 acgagcatgg cctcgaagaa gaagtagaac aggaacacgt cggtggccga gaagacaccg
     3901 aagcacatcg tctcgaggac caggatcagc gcgaagtagg ccttgacgct gcgcttgccg
     3961 gcgtcggcgt cgtgccaggc ggccaggatg cagatcggcg tgaggatcgc ggtcaggccg
     4021 agcagcacga gcgcgatgcc gtcgacgccc acggcgtagg agatcccgaa ctgcgggatc
     4081 caggcgtggg tctcggtcag ctggaagccg ctgacgtcgg ggtcaaagcg cagcgccacg
     4141 gccagggtga gcaccagggc caccaacgag acggcgagcg ccacctgctt ggccagcagg
     4201 tcgcggccac ggggcagcag cgctaccccg accgccccaa cgagcgggac ggcgccgagg
     4261 acggtcagcc agggaaagtc ggtcacgtca gagcctcacg agcacgaggg ccgcgaccac
     4321 gagagccgcg ccggcgaaca tcgacagcgc gtaggagcgc acgaacccgg tctgcagccg
     4381 tcgcagccgc ccggacgacc cgccgacgag agcggccagg ccgttgacga gaccgtcgac
     4441 cccccggttg tcgaagaaga cgagcgagcg ggtgaggtat tgccccggtc gcatgaaggt
     4501 cgcctcgttg aaggcgtcct ggtagaggtc gcgccgggcg gcgaccgtga gccagcctgc
     4561 cgcagggacc gtcctgggca ccgagcgcgc cgcgtaggtg acccaggcca gcccggcgcc
     4621 cacggcgacg agcacgagcg tgaccccgat gatgaccggc tccgagatca ccggctcggc
     4681 ctcaccgtgt gagccggtga ccggctcgag ccagctcgtg atccacccgg tcgggccgag
     4741 gatgacgccg aggaacgccg agcccagcgc caacaccatc attggcagcg tcatgacgag
     4801 cggggactcg tgcgggtgca cgtcctcgtc ccagcgccgc tcaccgtgga aggtcatggc
     4861 aaacagccgg gtcatgtaga acgcggtgat cccggcgccg agcagggccg ccccgccgaa
     4921 gacccacggc cgccagccct cgccgatgaa cgccgcctcg atgatcttgt ccttgctcca
     4981 gaagccggac agcagcggga agccgatgat cgacagccag ccgagcccga aggtcaccca
     5041 ggtgatcttc atgaccgcgg acagcccgcc gaagtggcgc atgtcgatgc ggttgttcat
     5101 cccgtgcatc accgaaccgg cgccgaggaa cagcccggcc ttgaagaagc cgtgggtgag
     5161 caggtggaag atcgcgaagg cggcgccgac cgggccgagc ccggcggcca gcatcatgta
     5221 gccgatctgg ctcatcgtcg aggcggccag cgccttcttg aggtcgtcct tcgcgcagcc
     5281 gatgatcgcc ccgaagagca gcgtgatgac gccgacgatc accacgagca gctgggcggt
     5341 cggggctccg tcaaagatcg gcgcggagcg cacgatgagg tagacgccgg cggtcaccat
     5401 ggtcgcggcg tggatgagcg ccgagaccgg cgtcgggccg gccatcgcgt cgccgagcca
     5461 ggactgcagc gggaactgcg ccgacttgcc gcacgcggcc agcagcagca tcagcccgat
     5521 cgcggtgagg atgccctcgc tcgccccctc cgcgccggca aagacgccgg cgaagtcggt
     5581 gcgcccgaac gtcgcgaaca tgatcatgat ggcgatcgag agccccacgt caccgacgcg
     5641 gttgaccacg aacgccttct tggccgcggc ggcgtaggtc gggttgtggt tccagaaccc
     5701 gatgagcagg taggacgcca gcccgacgcc ctcccagccg acgtagagcg tgaggtagcc
     5761 gtcggagagg accagcagca gcatcgccgc gacgaacagg ttgaggtagg cgaagaaccg
     5821 gcgacggtcc gggtcgtcgg ccatgtagcc gatcgagtag acgtggatca ggctgccgac
     5881 gaaggtcacc agcagcacga aggccagcga cagcgggtcg accagcaggc tcgcgtcgac
     5941 ctggaaaccc tcgaccggaa tccagctgaa caggtgctgg gtgaccgctc gggcgtcgct
     6001 gtcgcggccg agcatcgcga cgaacaggat cgcggcgacg acgaaggagg cccacgaggc
     6061 ggccgtgccg acgatgtgcc cgaccgggtt ggtgcgtcgg ccgccgacca gcaggaccgc
     6121 ggcgccggcg agcgggagcg cgaccagcag ccaggtcagc gagaacgccc cgctggcctg
     6181 tgccgtctcg agcgccgccg cggcgtggat gctcgtcacc tacgacccct cagtatttca
     6241 acaggttcgc gtcgtcgacc gaggccgagc gacgggtccg gaagatcgtg atgatgatcg
     6301 ccaggccgac gaccacctcg gcggcggcga ccaccatgac gaagaacgcg atgatctgcc
     6361 cgtcgaggtt gccctgcatg cgggcgaacg tgacgaaggc caggttggcc gcgttgagca
     6421 tgagctcgat gcacatgaac acgacgatcg cgttgcgccg cagcaggacc cctgcgccgc
     6481 cgatgctgaa cagcagggcc gacaggtaga ggtagttgac cgggctcact gtgcgtcctc
     6541 cgaagagtcc gtgatcgcct cggtgaccgg ccgggtgtcg aactcgtgga tgtcacggac
     6601 gtcgccgcgt gccgcgagga cccggttgat ggtcagctcg ctcgggcgcc cgtcgggcag
     6661 caggcccggc acgtcgacgg cgttgtgccg ggcgtagacg ccgggtgccg gcagcatggt
     6721 caccagcccg cccgagcgga agcgcgcctc ggccagctcc cgctgggtca gccggcgcgc
     6781 caggcgctcc cggtgggcca tcaccatggt gcccagcgcc gccgtgatca gcagcgcgct
     6841 ggtcacctcg aacccccaga cataacgggt gaagagcatc tcggcgacgg cggggacgtt
     6901 gccctcggcg ttggcctggg tcagcccggt cccgtcggcg aagacggccc gtccgatccc
     6961 gccgaccagc agcagggcga acgcgaccgc cgcgagcagg ccagcgaccc gttggccgcg
     7021 cagcgtctcg acgagcgagt cagacgagtc gaccccgacg agcatgagca cgaagaggaa
     7081 cagcatcatg accgcgccgg tgtagacgac cacctgcacg atgccgagga acggcgcctg
     7141 ctgggcgatg tagaacagcg ccagacagat catgatcagc gccagcatca gcgcgctgtg
     7201 cacggccttc ttggccatca ccaggccgag cgcagccagc accgagagcg gcgcgcagat
     7261 ccagaacagc caggcctctc cggcgccggt ctgggcggcc gcgaggaccg tgggggcgga
     7321 ggcgatcacg gccgggcctc gcctgctgcg gcggagtcgg gcgtgccgtc gccgggggtg
     7381 agcaccgggc cctgctcggg gacggcgcgg gtgacccggc cgtggtagta gtccttgtca
     7441 gtggtgcccg gagccatcgc gtgcggcggc gccaccatgc ccggcagcag cggtgccagc
     7501 aggtcgtgct tctcgtagat gaggctctcg cgactgctgt cggccagctc gaactcgttg
     7561 gtcatcgtca gggcgcgcgt cgggcaggcc tcgacgcaca tcgcgcagaa gatgcagcgc
     7621 aggtagttga tctggtagac gcggccatag cgctcaccgg gcgagaagcg ctcctcgtcg
     7681 gtgttgtcgg cggcttccac gtagatcgcg tcggccgggc aggcccaggc gcacagctcg
     7741 cagccgatgc acttctccag cccgtcgggg tgccggttga ggacgtggcg gccgtggaag
     7801 cggggcgcgg tctgcttctt ctcctcgggg tactgctcgg tgacgacctt gcggaacatc
     7861 gtcgagaagg tcaccccgaa ccccgcgatc gggtcgagga acttaggcat cggtcacctc
     7921 ctgacgctcg ctggtgggtc cgtccggact ggcgtcgcgc accgagcggc gtgcgctcgc
     7981 ggtggcatag gccggcagca cctgtccagg catcggcggg acagggtatc cgccggccat
     8041 cgggtcgaac tcgacgggct cggtggactc ctccggctcg ccggcccgcc gcgcggccca
     8101 cgcgtcccag gccatggtcg cgaggaacac cacggcgagc ccgccgccga cccagatgaa
     8161 cagggctcgg cggtcgaagg tcggctcgtt ggagatggcc cggatcgcag ccaccgcgag
     8221 gatccacgcc agcgacaacg ggatcagcag cttccagccc agcttcatga actggtcgta
     8281 gcgcagccgc ggcagggtgc cgcgcagcca gatgaacacg aagatgaaca ggttgatctt
     8341 gagcaggaac cagagcagcg gccaccagcc ggtgttgagc agaccggtcg ggtgcagctc
     8401 gtcgacgccg atcgccgaca gcggccacgg cgcccgccag ccgccgagga agagggtggt
     8461 ggccagcgtg gagacggtga ccatgttgat gtactcggcc aggaagaaca gcgcgaactt
     8521 cagcgacgag tactcggtgt ggaacccgcc gaccagctcg ccctcggcct cgggcaggtc
     8581 gaacggcgcc cggttggtct cgccgaccat cgcggtcatg tagatgagga acgacgggat
     8641 cagcggcagc gcgaaccaca ggctggtctg cgcgtcgacg atctgcgagg tcgacatcga
     8701 gccggcgaag aggaacaccc cgacgaagga caggcccatg gccacctcgt aggagatgac
     8761 ctgggcgctc gagcgcagcc cgcccagcag cgggtaggtc gaaccgctcg accaaccggc
     8821 cagcacgatg ccgtagacgc cgacggacgt gaccgcgatg acgtagagca ccgcgatcgg
     8881 catgtcggtc agctgcagca tggtggagtg gccgaagatc gacaccttcg ggccgaacgg
     8941 catcacggca taggccatga acgccggtac ggcggccagc accggcgcca ggatgaagac
     9001 caccttgtcg gcggtcttgg ggatgaggtc ttccttcagg gccagcttga tgccgtcggc
     9061 gaggctctgc agcaggccct gcgggccgag ccggttgggg ccgatccggt gctgcatgcg
     9121 ggcgaccacc cgccgctccc accagatcgc gaacagcgtc atcacgacga ggaacacgaa
     9181 gatggccacg gccttgagca gcaccagcca ccacgggtcg gtgccgaaca agcccgtcgc
     9241 cggggccgac tcggcggcca gcaggtgccg gctcatgcgg cgctccccgc ggtgatcgtg
     9301 acgacggcac cggtgtcgac cgcgagcgtg ggccgcacct gagagccgac cgagttggtg
     9361 ggcacccaca cgacgcggtc gggcatcgcg gtgatcgtca gcggcagggt cagctcgccc
     9421 gcggcggtgg cgacggtgac cttgtcgccg tcggcggcac cgatctcggc cgcggtggcg
     9481 gccgagagcc gcaccgcgct ggcgtgcgcc gtgccggcca ggaacggctc accggcctgc
     9541 agcgtgccga gatcgagcag ctgcgcccag gtggccagca ccgcctggcc cggaccgggc
     9601 tcgggcggtg ggccggcctc gacgctgggg gcgggcaccc gcgcaccgtc ccaggcggcc
     9661 agctcggcga gttctcggcg ggcctcggcc acgtcacgca ccccgatcgg cgcgtccagc
     9721 tcgtcggcga gcaccgagag cacccgcagg tcgggcatcg cgctggtcga cagcacggtg
     9781 ccgaacacgc gctcccggcc ctcccagtcg aggaaggtgc cggacttctc gacgaccggg
     9841 gccaccggca gcaccacgtc ggcccggctg gtcacggcgc tcacccgctg ctcgaggctg
     9901 acgaggaagg gcacccggtc gagggcctcc agcgccaaca ccgggtcggg caggtcgtcg
     9961 gggtcgaccc cgccgacgac gagtccgccg agcgcgccgt cgcgggccgc ggtcagcatc
    10021 gtggtcgcgt cgcggccggg ctgcgccggc acggagacgc cccacacggt gccgacgtcg
    10081 acccgggcgg gcgcgtcgtc gaccggccgg ccaccaggca gcagcccggg cagcagcccg
    10141 gcctcgaggg ccccgcggtc accggcccgg cgcggcaccc aggccagccg ggcgccggtc
    10201 tcggccacca gggcgagtac ggcggagagg gtgccggcca cggaggcggc ccgctccccg
    10261 acgagcacga tcgcgccggg gcggcgtacg gcctcggcca ccgccgccac cgcctcgtcg
    10321 gccgcggtga tcgcggccag cacctcgggc tcggtgcccg gtgcggtcgg cagcagccgt
    10381 ccgcccatct tgtgcagccc gcgggtggcg tagggcgcca ccgagaacac cggcaccgac
    10441 ttcttgcgca tccccttgcg cagccggagg aacaccatcg gcgactcctc ctcgggctcg
    10501 aacccgacga gcaggacggc cggggcgtgc tcgaggtcgg cgtaggtgac cccgccggtg
    10561 gccgggctgg tgcccacgat cgcgtgcccg aggaaggcgg cctcctcggc cgagtgcggc
    10621 cgggcccgga agtcgacgtc gttggtgccc agcgcgagcc gggcgaactt ggaccaggcg
    10681 tagccgtcct cgagcgtgac ccgcccgccg ggcagcacgc cgaccccggc accgtcgcgg
    10741 gcggcccgca ggccggccgc ggcgacggcc agcgcgtcgg gccacgaggt cgggaccagc
    10801 tcgccggact cctcgtcgcg gacgaggggc gtggtgatcc gctcaggcat cgtggcgtac
    10861 tggaacgccc agcggccctt gtcgcagttc cactcctcgt tgaccgccgg gtcgtcgccg
    10921 gccatccgcc gcagcacctt gccgcggcgg tggtcggtgc ggatcgcgca gccggacgcg
    10981 cagtgctcgc agatgctcgg cgtggacacc aggtcgaacg ggcgtgaccg gaaccggtag
    11041 gcggcgccgg tcagcgcgcc gaccgggcag atctggacgg tgttgccgga gaagtaggag
    11101 tcgaacggct ccttctcata gatgcccacc tgctgcagcg caccacgctc gaccatgtcg
    11161 atgaaggggt cgccggcgat ctggtcggag aagcgggtgc agcgggcgca caggatgcag
    11221 cgctcccggt cgagcagcac ctgcgaggag atggcgatcg gcttggggta ggtgcgcttg
    11281 acgccctcga agcgcgactc ggcccggccg ttgctcatcg cctggttctg caacgggcac
    11341 tcgccaccct tgtcgcagac cgggcagtcg agcgggtggt tgatgagcag cagctccatc
    11401 acgccgtact gcgccttctc ggcgaccggc gaggtgagct gggtcttgac caccatgccc
    11461 tcggagaccg tggtcgtgca cgaggcgagc ggcttgcgct gacccgccac ctcgaccagg
    11521 cactgccggc aggcgccgac cgggtcgagc agcgggtggt cgcagaaccg ggggatctgg
    11581 gtgccgatca tctcggcggc ccggatcacg agcgtgccct tggggacgct gaccacgaca
    11641 ccgtcgatcg tcagggtgac gaggtcctcg ccctgctggg cgccggcgcc gggggtgctc
    11701 gtcacggtca tgacgcactc gctccagtgg aagacgtgga cgacgcggcg gcgaacaccg
    11761 tggacgccgc cgggtcgaac gggcagccgc cgtgggtgaa gtgggcgatg tactcgtcgc
    11821 ggaagtactg gatcgacgag gtcaccgagc tggtcgcgcc gtcgccgagc gcgcagaagg
    11881 cccgcccgag gatgttgtcc gacaggtcga gcagcttttc gaggtcttcc tcggtgccgt
    11941 tgcccgcctc gagccggtcg aagatctgca cgtaccagta ggtgccctcg cggcacggcg
    12001 tgcacttgcc gcaggactca tgggcgtaga acgcgctcca ccgccgtacg gcgcgcacga
    12061 cgcacgtggt ctcgtcgaag atctgcagcg ccttggtgcc gagcatcgag ccggccccgc
    12121 cgacgccctc gtagtcgagc gggacgtcga ggtggtcggc ggtcaagatc ggcgtcgagg
    12181 agccgccggg ggtccagaac ttgagctcgt ggccctcgcg gaccccgccg gccagctcga
    12241 gcagctcgcg cagggtgcag cccatcgcgg cctcgtactg gccgggccgg gtgacgtgcc
    12301 cggacagcga gtagatcgtc atgcccttgg acttctcggt gcccatgccg gcgaaccagc
    12361 tcgggccgtt gc
//
LOCUS       DATIBB010000083         2200 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_1753643, whole genome shotgun sequence.
ACCESSION   DATIBB010000083 DATIBB010000000
VERSION     DATIBB010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2200
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1753643"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..209
                     /locus_tag="VLC50_02960"
                     /pseudo
     CDS             1..209
                     /locus_tag="VLC50_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611352.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            complement(623..>2200)
                     /locus_tag="VLC50_02965"
     CDS             complement(623..>2200)
                     /locus_tag="VLC50_02965"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HSF26457.1"
                     /translation="DRLRRQAAVLQVLDHSHVVRLHRLLLSEGEVVLVTGLAAGGSLD
                     GLLARRGRLSAGEVVTIGTPLAQALAAVHARGLVHGTVGPRAVLFTAEGRPLWSDLGL
                     AWARGTTCSAADDVYRLGALLRDAFDGAPALGGDLPEADEAGVLGAPEPVLPPALASA
                     LTAACDPDPTARPSAAELAVMLYDAVAATPVRLAASNTADDSTRSLSPTIDRAPAVSA
                     PARDLPDVVVLGSSPSQPRSLRRSVASGSARSRTRSLRRPVASSRARSRTRGRLRSWR
                     PPVGRARGPFVAMVVAAVLLLAVGGWRWWSPGGALPSEAALTTSVPTEGVSVSTGDGS
                     GSTSSGSAPTSRGPVSGTSGSMSSSGELPDTAKPVDPDVAAAQVLAQLDAKRSVTYAN
                     GDLDALGLLYTPWSLSRDVDMGNLQRLVDAGERAQGFRTDAISVRVITRSPDAMELAV
                     VDTIPQFTVVGANGTPVRVESGRAERSFRIALERVDGQWLYDTVDADTADADTVDADT
                     ADADTLGIDAADAAKRS"
BASE COUNT          366 a          833 c          741 g          260 t
ORIGIN      
        1 ttgcggccag catgggcagc aagggagacg cctacgacaa cgccatgatg gaaagtttct
       61 tcggcaccct gaagatcgaa ctcatcgacc ggcgtcgctg ggcaacccgc ggcgaggtcg
      121 agatcgcgct gatgtcctac ctcgagggct ggtacaaccc tcggcgcctc caacaagccc
      181 tgggatggcg cagcccgctc gagtacgagg ccgcccacca cgccggccac gacctgtcgg
      241 tgcccgccac ctgccagcct gcgcccgtcc tcgccgtcgt caaggccaag ccctccgggc
      301 ggcctccggc cggccttgac cccggctacg ggcaggcgcc caatggcaac ggcggcgcca
      361 ccggcggccc acaaccgaaa ccggccgacc cggtcggaac cacctaaccg gtctccaaga
      421 aagcggggga ggccctcctg cctgtgccga cgccaccatc ggctcaagca ggcccgccgc
      481 tggcacttgc agctgcactc cgatggcacc gccacttgga ccagccccac cggccaggtc
      541 aggctcaccc gcccaaccca gcgaaccctc cggcacgatt gacgcggttg ggaaggtgcg
      601 cacgcgggcg agggacggtg gtctacgaac gcttggcagc atccgcggcg tcgatgccca
      661 gcgtgtcggc atccgccgtg tcggcgtcca ccgtgtcggc atccgccgtg tcggcgtcca
      721 ccgtgtcgta gagccactga ccgtcgacgc gctcgagcgc gatccggaag ctgcgttccg
      781 ctcggccgga ttcgactcgc acgggagtgc cattggcgcc cacgacggtg aactgcggaa
      841 tcgtgtcgac gaccgccagc tccatggcat ccgggctcct ggtgatcaca cggacggaga
      901 tggcatcggt gcgaaatcct tgggcccgct cgccggcgtc gacaaggcgt tggaggttgc
      961 ccatgtcgac gtcacgcgac aacgaccagg gggtgtaaag caggcccagc gcgtcaagat
     1021 caccgttggc gtaggtcacg gatcgcttcg cgtcgagctg tgccagcacc tgcgctgctg
     1081 cgacgtcggg gtcgacaggc ttggcggtgt cgggcagctc accactgctg gacatcgagc
     1141 cgctagtgcc ggagacgggg ccccggctgg ttggcgccga gccactgctg gtggagcccg
     1201 agccatcgcc ggtggagaca gaaacgcctt cggttggcac cgaggtcgtc aaggccgctt
     1261 cggacggtag ggcaccccca ggtgaccacc accgccaacc gcccaccgcg agcagcaaca
     1321 cggcggccac aaccatcgcg acgaaagggc ctcgggctcg acccaccggc ggccgccatg
     1381 agcgaagtcg cccccgcgtc cgagaccgcg cacgactcga cgcgaccggc cgacgcagcg
     1441 accgcgtccg agaccgcgca ctacccgacg cgaccgaacg acgcagcgac cgcggctgcg
     1501 acggcgacga tcccagcacc acgacgtccg ggaggtcgcg cgccggagcc gacacggccg
     1561 gcgcgcggtc gattgtcggg ctcagcgaac gcgtcgagtc gtccgccgtg ttcgacgccg
     1621 ccaggcgcac cggggttgcc gccaccgcgt cgtagagcat cacggccagc tcggccgcac
     1681 tcggccgggc cgtcggatcg gggtcgcagg ccgcggtgag cgcgctggcc agagccggcg
     1741 ggagcaccgg ctcgggggcg cccagcacac ccgcctcgtc ggcctcgggc aggtcgccgc
     1801 ccagagcggg cgccccatcg aacgcgtcgc gcagcaaggc acccaggcga tacacgtcat
     1861 cagccgcaga gcaggtggta ccccgagccc aggccagccc gaggtccgac cagagcggcc
     1921 ggccctcagc ggtgaacagc acggcccgcg gaccgaccgt gccgtggacc agaccgcgcg
     1981 cgtgcaccgc ggccagcgcc tgtgccagcg gcgtgccgat cgtcaccacc tcaccggcac
     2041 tcagccgtcc ccgccgcgcc agcagaccgt caaggctccc gccggcggcc agcccggtca
     2101 ccagcacgac ctcgccctcg ctgagcagca gccggtgcag gcgcacgaca tgcgagtggt
     2161 cgagcacctg cagtacggcg gcctgccggc gcagccgatc
//
LOCUS       DATIBB010000084        19466 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_570397, whole genome shotgun sequence.
ACCESSION   DATIBB010000084 DATIBB010000000
VERSION     DATIBB010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 19466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19466
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_570397"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(146..712)
                     /locus_tag="VLC50_02970"
     CDS             complement(146..712)
                     /locus_tag="VLC50_02970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF26458.1"
                     /translation="MVEGSGSTRNSAADDPGYLRSITSKTVLGQFRLIMVAILLVPIA
                     VIGLVPLVAGVGASPVLYVVLPPLLAISGFVMARMLVLRSAPPLPVRLPHGEDHGATA
                     VAALRQATYLPLALTEAPLLIGFMLSVVAGSTLPYLAGFVVGWPLMVVASLPTHTRIE
                     RIRASLESQGATSHLWDVLLSAPRSYVH"
     gene            828..2060
                     /locus_tag="VLC50_02975"
     CDS             828..2060
                     /locus_tag="VLC50_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014135624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HSF26459.1"
                     /translation="MNRPSAATVRRVERATGSLAQAALARMDDELPWYRAMGPAERSW
                     VGLVAQAGIAAFVAWFREPYAGPAITADVFGTAPRELTRAVTLQQTVEMVRVTIDVVE
                     SRIDDIVTPVEAPQLREAVLRYSREVAFAAADVYARAAEARGAWDARLEALVVDAVLR
                     GEADESVRSRAAALGWGSGNRVAVVVGHPPERATRSPLDAEEVVDDVRRHARAAGLDV
                     LTGVQGDRLVAVLGGLTDALDAARSVAASFAPGPVVVGPVVTDLFSGAASARAAVAGL
                     RAVAGWADAPRPVAAEDLLPERALAGDGHARRALVAEVYAPMLAAGPSLLETLAAYLE
                     HGSSVEATARLLFVHPNTVRYRLRRVVDVTGRAPTDPRDAYTLRLALTLGRLMSPAAD
                     GTTFVGSPQEAAGEVRRR"
     gene            2099..3289
                     /locus_tag="VLC50_02980"
     CDS             2099..3289
                     /locus_tag="VLC50_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020553209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
                     /protein_id="HSF26460.1"
                     /translation="MLVVVAPGQGSQTPGFLTPWLEVPGFADRMRWLSAVAGIDLVRH
                     GTESDAETIRDTAVAQPLLVAAAMVSAIALFPHPGDAFKAVGAVAGHSVGEIAAAAGA
                     GVISAEQAMVFVRERGRAMAEAAAVTPTGMTAVLGGDPDEVLAKLAEHGLTPANANGA
                     GQVVAAGTLAQLAALKADPPTKARLIPLQVAGAFHTSHMAPAVGLLESHARAISTHDP
                     RTRLISNADGTVVHNGRDMLARLVRQVSAPVRWDLCMQTMADLGVTAVIEMPPAGTLS
                     KLIKRAIPGVETLALKTPDDLPAARELVERHGTASGLDISPTWRLLVSPASGTFVRPT
                     NGAEVGATLDHGSAVGAVRTRRDERVVVAPHGGTVVEWLVEDGDPVSPGQPLVRLHPM
                     STGV"
     gene            3307..4308
                     /locus_tag="VLC50_02985"
     CDS             3307..4308
                     /locus_tag="VLC50_02985"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020553210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HSF26461.1"
                     /translation="MTSPITTSEGARYARVLGVGGYRPARVVTNHEIVDNIDSSDQWI
                     RERSGIVSRRWAAPDESVIDMSVAAAGKALANAGVAPDQVGFVLLATVTHPYQTPSAA
                     AEVAHRIGAVNAAAMDISAACAGFCYGLSLANDMVRGGSAGYVVVIGCEKLSDFTDHF
                     DRSTAFIFGDGAGAAVVGPSDVPAIGPTVWGAQGEHAGLITQKHSWIDVRDKAAEFPT
                     LTMQGQSVFRWAVWQMAPIAQQALDAAGVRAEDLDAFIPHQANMRIIDSMIKALKLPA
                     HVPVARDIAETGNTSAASIPLAMERMLESGEAKSGGTALIIGFGAGLVYAAQVLTLP"
     gene            4385..4633
                     /locus_tag="VLC50_02990"
     CDS             4385..4633
                     /locus_tag="VLC50_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013771421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HSF26462.1"
                     /translation="MAQSEQEILTGLAEIVNEETGIPTSDVAPDKSFTDDLDIDSLSM
                     MTIVVNAEDKFGVKIPDDEVKNLKTVGDAVAFIQRAGS"
     gene            4746..5990
                     /gene="fabF"
                     /locus_tag="VLC50_02995"
     CDS             4746..5990
                     /gene="fabF"
                     /locus_tag="VLC50_02995"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008748218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HSF26463.1"
                     /translation="MSTNEPIVVTGIGATTPLGGDVASTWSGLLAGRSGARTITEDWV
                     EALPVQFACPIAVEPAEVLSVPERKRLDRSGQFALTAAREAWADAGSPDVPGERLGVV
                     VASGIGGVTTLLAAYDTLKERGARRVLPMTVPMLMPNGPAAGVGLELGARAGVHTPVS
                     ACASGAEAIGYGVDMIRSGRADVVVAGGTEAAIHALPIAGFAAMQALSTRNDEPTRAS
                     RPYDKGRDGFVLGEGAGIVVLETLSSARARGATIYGEVAGVGLTADAHHIAQPDPEGL
                     GATRAMRLALEAAGATARDVVHINAHATSTPVGDIAEAKALHAALGDAVGQVVVTSTK
                     SMTGHLLGAAGAIETIATLLALRERTVPATINLEDPDDDIGLDVARNGARALPADGPL
                     LALNNSFGFGGHNIALALRTVS"
     gene            6071..7423
                     /locus_tag="VLC50_03000"
     CDS             6071..7423
                     /locus_tag="VLC50_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011755443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HSF26464.1"
                     /translation="MRLGRFFDPGTVELITPVDRSGMIAATGLVEGSPVTAFASDATV
                     MGGAMGVDGCRVIVTAYEHALETGTPVVGLWHSGGARLPEGVVSLHAVGEVFAIMTRA
                     SGRIPQISVVLGPAAGGAAYGPALTDVVILGPDGRIFVTGPDVVRSVTGENVDALRLG
                     GPEPHGRRSGVVHVVAPTDDDAYDEARRLAALLGDQGQLDAATIEDVDLGEHLPESPK
                     RAYDVHPLIDGLLDRPGLELHPRWAPNVVTTLGRFGGRTVGVIANNPLRLGGCLDSSS
                     AEKAARFVRMCDAFGVPLVVIVDVPGYLPGVGQEWDGVVRRGAKLLHAFAEASVPRVT
                     LVTHKAYGGAYIAMNSRSLGATKVFAWPGAEVAVMGAVAAVRILHRRRLAEVPEESRA
                     DVELELAAEHEKLAGGLERAVEIGVVDEVVEPKLTRSTLARAIAAVPATRGQHANIPL
                     "
     gene            complement(7463..7978)
                     /locus_tag="VLC50_03005"
     CDS             complement(7463..7978)
                     /locus_tag="VLC50_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011755441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3145 domain-containing protein"
                     /protein_id="HSF26465.1"
                     /translation="MSAAHPQPSARGVVYVHSAPPALAPHVEWAIAGVLGVRVSLDWT
                     PQPAAPGLLRAELSWQGSPSTGSKIASALRGWARLRYEVTEEPSPGCEGERYSCTPDL
                     GIFHAVTGVHGDLLVPEDRLRAAMVRAAAGEADLTEEVDRLLGRPWDEELEPFRYAGD
                     GAPVRWLHQVV"
     gene            8205..8630
                     /locus_tag="VLC50_03010"
     CDS             8205..8630
                     /locus_tag="VLC50_03010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF26466.1"
                     /translation="MTDAELLMAAAYDEGVVSEVIRPAAIIPEEAARAVLVELALRDV
                     QNGGNWQSEPSLWSRFDRPFHGPNNPAGAELIGTIQVAYGTPTRYEITIYRATVTRLG
                     TENGWTVEALCDEALAFGNLSLATCPRAKLAAPPKPFRF"
     gene            complement(8659..9144)
                     /locus_tag="VLC50_03015"
     CDS             complement(8659..9144)
                     /locus_tag="VLC50_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009154782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HSF26467.1"
                     /translation="MSATSGRRSAGLLLWRRQAGEVEVLLGHMGGPFWSRKDAGAWTI
                     PKGEYDATEDPLVAARREFVEELGQPVPDAEPVPLGEVRQSGGKRVLAWAVEGDLDPA
                     TMVSNTVEIEWPPRSGRRMLVPELDRCAWFPLPAAHDALLVGQRPLLERLTALLAPPQ
                     A"
     gene            complement(9141..9809)
                     /locus_tag="VLC50_03020"
     CDS             complement(9141..9809)
                     /locus_tag="VLC50_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019634586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine synthase"
                     /protein_id="HSF26468.1"
                     /translation="MPARPSAPTVLARAAGLSGELVLRRHGEHFEVVANGVFLMDTRD
                     GRSERLLVEASLAAAPEPSGPLEVVVGGLGVGFSLLEVLAHPTVGRVVVVELEPVLVE
                     WHRGPLAAVTAGALDDPRVQVVCADITAWLAQAHASYDVICLDVDNGPDWLVRPDNAA
                     LYDDAGLRALAGALRPGGVAGIWSATGSETFEARLGQHFAQVRTHRVDVARGGPDVVW
                     VVSR"
     gene            complement(9820..10176)
                     /gene="fdxA"
                     /locus_tag="VLC50_03025"
     CDS             complement(9820..10176)
                     /gene="fdxA"
                     /locus_tag="VLC50_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003875342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HSF26469.1"
                     /translation="MTYVIAEPCIDVMDRACVDECPVDCIYEGGRSLYIHPDECVDCG
                     ACEPVCPVTAIYYEDDLPGGFELYKEDNAAFFSETLPGHDEPLGSPGGAAKLGPRGVD
                     TPLVAAHPAAEGADGA"
     gene            10308..11048
                     /locus_tag="VLC50_03030"
     CDS             10308..11048
                     /locus_tag="VLC50_03030"
                     /inference="COORDINATES: protein motif:HMM:NF013488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HSF26470.1"
                     /translation="MSVVIYGDFTCPYSYLASRRTDALIEAGVDVDWHAVERRPDLPV
                     TGVALTPDSRAALEAEVAEVTELLRPGEELPLEIHGTIPRTEAAVSGYAEAYGVGTAA
                     DVRRLLFRTYWVEGGAIGDPEVLRQLLVGPILRGDGGAGPLHEFGYAVAVSRGPMTTA
                     AFRHIQAWRAGWVATGTGTTPTVVVDGTVHAGVAGLHWLADEIDRLGAPRAPEPAEPS
                     RYPEVSLRPQPEWVSTVGGPWARAFMHR"
     gene            complement(11060..11503)
                     /locus_tag="VLC50_03035"
     CDS             complement(11060..11503)
                     /locus_tag="VLC50_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015354603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family peroxiredoxin"
                     /protein_id="HSF26471.1"
                     /translation="MAIAERSAQTVWEGPLASGAGVITGNSGAYGELPVTWASRTEAP
                     GGKTSPEELAAAAHSSCFSMALSLRLGEHGLTPTRLSVTATVALDAVDGLPTVVSSDV
                     DVRAVVPGADVTTFQAAVDEAAALCPISRLFAGAKIAVTAVLETA"
     gene            11730..12071
                     /locus_tag="VLC50_03040"
     CDS             11730..12071
                     /locus_tag="VLC50_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007824243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lsr2 family protein"
                     /protein_id="HSF26472.1"
                     /translation="MAQKTIVQLVDDLDGKELTAQQGQTVTFGLDGVSYEIDLSNKNA
                     KALRDLLGRYADVARKVGTARAGSGRIKRTQLGPDAVVVKAWADSKGLDYPKRGRLPK
                     ALLEQYAADHR"
     gene            12229..12810
                     /locus_tag="VLC50_03045"
     CDS             12229..12810
                     /locus_tag="VLC50_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017608611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HSF26473.1"
                     /translation="MAVKSPKSSQTSQPWLHQKGAQIQKFGTVRQLPIGLSQETRAYS
                     CERLNACLADSQILYGLYKKHHWLMRGPTFYQLHLLLDKHAGEQLLLIDSIAERVQTL
                     GGVAIGDPRHVAEITTVPRPPDGVEEVPAMLSRLLEAHEIILEQAHDAAARADKMGDD
                     GTNDLLVSDVIRTNELQAWFLAEHLVDTPLVHA"
     gene            complement(12864..13136)
                     /locus_tag="VLC50_03050"
     CDS             complement(12864..13136)
                     /locus_tag="VLC50_03050"
                     /inference="COORDINATES: protein motif:HMM:NF040451.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6510 family protein"
                     /protein_id="HSF26474.1"
                     /translation="MDTSPTFLDGNAAAGPMADVLGFDITVAVGRCAGCGESAVMAQC
                     RVYSAGPGLVLRCSSCEHVLARLVVAGNRTWLEMTGIASLQIDSTA"
     gene            complement(13146..13874)
                     /locus_tag="VLC50_03055"
     CDS             complement(13146..13874)
                     /locus_tag="VLC50_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012892750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HSF26475.1"
                     /translation="MGRLSWRPATVAAIREETSTARSLALTVDGWPGHRPGQHVDLRL
                     TAQDGYTAVRPYSLARPTDGSRIDITVALVEDGEVSSYLVRGARIGTELEVRGPLGGW
                     FVWDREDPGPVQLLGGGVGVVPLTAMLREHDQVPDPHPMRLLYSTRSPEALLYGAELT
                     RRVAHGAPSSTVSVLYTRDAPDDAARPPGRLSASDIAAYALPPSPRTRCYVCGPTGFV
                     ETGITLLEAAGFPASNIRAERFGP"
     gene            complement(13862..14467)
                     /locus_tag="VLC50_03060"
     CDS             complement(13862..14467)
                     /locus_tag="VLC50_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018787245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite oxidase-like oxidoreductase"
                     /protein_id="HSF26476.1"
                     /translation="MSSFSEGFMGRRNRRAGGTELPPGQYLTHDFPVLSAGATPRVPL
                     ESWQLEVSGEEGVAASWSWDEFRALPAEEVTVDLHCVTRWSKLGTTWTGVPVETLLDE
                     ADVDGSFVLASSYGGYTTNLPLEDLLDGKAWVAFDYDGEPLDPEHGGPARLLVPHLYL
                     WKSAKWLRGLRVQDEDEPGFWEQLGYHEYGDPWREQRQWGD"
     gene            complement(14653..16044)
                     /locus_tag="VLC50_03065"
     CDS             complement(14653..16044)
                     /locus_tag="VLC50_03065"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HSF26477.1"
                     /translation="MGWLLGHTFLLLVHVGRKTGKPHATAAMVIARDKSTGEVVICSV
                     WGARADWVQNLRAHPALRVQIGRASFVPEQRFLTSDEAFAVAVEFRRRHPRRLRLLSR
                     VLGWDDLRSEAAMREFIATRPFVSLRPTSPKDPTMATMWAIVQDKYGDDADLLRHERI
                     ARPEVADNEVLVRVHAAGLDRGTWHLMTGRPYLMRIAGLGVRGPRDRVSGRDLAGTVE
                     AVGSAVTRFEVGDEVYGVGRGSFAEYAAAKQDKLARKPVNLSFEQAAVVPISAGTALQ
                     ALTDHGRVEAGQRVLVIGASGGVGSYAVQLAKALGAEVTGVASTAKLDLVRSLGADRV
                     LDYTSDDYANGTARYDLVLDIAGNPRLSRLRRALTPKGTAVLVGGESGGNLTGGMNRP
                     LRALVLSPFLGQRLVWFVAKERASDLERLTELIEAGRVTPSIDRTYPLDRVPDAMRHL
                     ASGQVRGKVAIIV"
     gene            16416..17339
                     /locus_tag="VLC50_03070"
     CDS             16416..17339
                     /locus_tag="VLC50_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020610001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSF26478.1"
                     /translation="MAILTRGLVKDFGRTRALDHLDLAISAGEVHGFLGPNGAGKTTT
                     IRILLGLLRRTTGDVMLLGGDPWRDTATLHRRLAYVPGEMNLWPNLTGGEVIDLLSSL
                     RGGLVPARRAALLEQFDLDPTKKCRTYSKGNRQKVALVAAFSSDVELYLLDEPTSGLD
                     PLMETQFQAVVRGLRANGKTILLSSHILSEVEALCDRVTIIRDGHVAESGTFAELRHL
                     TRTTVSIETTRPVAGLADLDGVHDLSVDFNRARFSVDADDLNRVLEHLVAYEVRSMTS
                     SPPTLEELFLRHYDTRGDSDRAPAGEVVLAR"
     gene            17336..18958
                     /locus_tag="VLC50_03075"
     CDS             17336..18958
                     /locus_tag="VLC50_03075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC antibiotics transporter"
                     /protein_id="HSF26479.1"
                     /translation="MNAMSGTIRLTRLAARRDRITIPVWILGLAAFLTATTAMFVNSL
                     ITHEDVVREAELPTNNVGLRLLGLTSGPTVGGAVMVRDYVWLAVMAALMSVLAVVRHT
                     RQSEELGRAEVVGSTVVGRYADLAAAVIIAVAANMVLAVSLALAMVVNGQPASGAFTA
                     GAGVAAVGVVFTGVAAVTVQLASTTRGAIGMAGAVLGVAFLLSGIGNMLGTADTRALR
                     VTSAWPAWLSPIGWAQQMRPFGGNHWWPLALFSVLFACLLVTARALVVRRDVGAGLWP
                     QRRGHATAAASLLSPTGLGFRLQRGALLGWAVGMLAFGLIFGAMSEQIQDVTGQAREF
                     YERFGGTDKILESYDASMAAMGAMVVAIYVVQMVLRMRAEEAAGSLESLLASGVTRPR
                     WFLGHLLNTVGGAVALILLYSAGMGIGAGQVLGGTPTQLGKTLTAGVVQVPAILLIGG
                     CALVAVAVLPRLASPLAWSVVIAALLLGPMFGPGLGVPEGVMDLSPFTHVPTVPATDA
                     SAPPLLILTGLCVAFGVIAAAAMRRRSLLLPA"
     gene            complement(19332..19405)
                     /locus_tag="VLC50_03080"
     tRNA            complement(19332..19405)
                     /locus_tag="VLC50_03080"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19369..19371),aa:Ile,seq:cat)
BASE COUNT         2767 a         7153 c         6813 g         2733 t
ORIGIN      
        1 tcgccctcgc ggcgctgacc gagctggtcc gccagggcga gctcaagagc gaggtacgcc
       61 gcgaggcgat cgagcgctac cagctgcgcg aggtcaccgc cggctcgact ggcttcgagg
      121 gcggcgacgc ctgacggccc cgggctcagt ggacgtagga gcgcggggct gagagcagca
      181 cgtcccacag gtgcgacgtg gcgccctgcg actccaggct ggcccggatc cgctcgatcc
      241 gcgtgtgggt cggcaacgag gcgaccacca tcagcggcca gccgaccacg aagccggcca
      301 ggtagggcag cgtgctgccg gccacgacgc tgagcatgaa cccgatcagc agcggggcct
      361 cggtcagcgc cagcggcagg taggtggcct ggcgcagtgc cgcgaccgcg gtcgcaccgt
      421 ggtcctcgcc gtgcggcagc cgcaccggca gcggcggggc cgaccggagc acgagcatgc
      481 gggccatcac gaaccccgag atggcgagca gcggtggcag caccacatag agcaccggtg
      541 atgcccccac cccggcgacc agcgggacca ggccgatcac cgcgatcggg acgagcaaga
      601 tggcgaccat gatcagccgg aactgcccca gcaccgtctt cgacgtgatg ctgcgcaggt
      661 agccgggatc gtctgcggcg ctgttgcggg tgcttccgga gccctcgacc atggcgtgac
      721 cttagcgacg cggcacgcca gcgccagagc agcacccggg gcgggcttgg gcgcggttgt
      781 gcctcccgga caaatgagta cgtcgcggcg ccgtacgctg cggtgtcgtg aaccgcccca
      841 gcgccgcgac ggtgcgccgg gtcgagcggg cgaccggctc gctggcccag gccgcgctgg
      901 cccggatgga cgacgagctg ccgtggtacc gcgccatggg cccggccgag cggtcctggg
      961 tcgggctggt cgcgcaggcc ggcatcgcgg cattcgtggc ctggttccgc gagccgtacg
     1021 ccgggccggc aatcaccgcc gacgtgttcg gcaccgcgcc gcgcgagctg acccgggcgg
     1081 tgacgctgca gcagaccgtc gagatggtcc gagtcaccat cgacgtggtc gagtcacgca
     1141 tcgacgacat cgtcacgccg gtcgaggcgc cgcagctgcg cgaggcggtg ctgcgctact
     1201 cccgcgaggt ggccttcgcc gcggccgacg tctacgcccg ggccgccgag gcgcgcgggg
     1261 cctgggacgc ccggctcgag gcgctcgtcg tcgacgcggt gctacgcggt gaggccgacg
     1321 agtcggtgcg ctcacgagca gccgcgctgg gctggggctc gggcaaccgg gtggcggtgg
     1381 tggtcggcca cccgccggag cgcgccaccc ggtccccctt ggacgccgag gaggtggtcg
     1441 acgacgtacg gcggcatgcc cgcgcggccg gcctcgacgt gctcaccggc gtgcagggcg
     1501 acaggctggt cgcggtgctc ggcgggctga ccgacgcgct ggacgccgca cgctcggtcg
     1561 ccgccagctt tgcccccggc ccggttgtgg tcggcccggt cgtcaccgat ctgttcagcg
     1621 gtgccgccag cgcccgggcc gccgtggccg ggctgcgcgc ggtcgccggg tgggccgacg
     1681 cgccgcggcc ggtggccgcc gaggacctgc tgcccgagcg tgccctcgcc ggtgacgggc
     1741 acgcccgccg cgccctggtc gccgaggtct acgcgccgat gctcgccgcc ggcccctcgc
     1801 tgctcgagac gctggccgcc tacctggagc acggctcgtc tgtggaggcc accgcccggc
     1861 tgctcttcgt gcaccccaac acggtgcgtt accggctgcg ccgggtggtc gacgtcaccg
     1921 gacgggcccc caccgacccc cgggacgcct acacgctgcg gctcgcgctg acgctgggac
     1981 ggctgatgtc ccctgcggcc gacgggacca cttttgtggg gtccccacaa gaagcggccg
     2041 gtgaagttcg tcggcggtga tcgccggtta ctcggccgta gcccgccaag gtggacccgt
     2101 gcttgttgtc gtcgctcccg gtcaggggtc ccagaccccc ggcttcctca ccccttggct
     2161 cgaggtgccc ggtttcgccg accggatgcg ctggctgtcg gctgtcgccg gcatcgacct
     2221 ggtccggcac ggcaccgagt ccgacgccga gacgatccgg gacaccgcgg tggcccagcc
     2281 gctgctggtg gccgccgcga tggtctcggc gatcgcgctc ttcccgcacc ccggcgacgc
     2341 attcaaggcg gtcggcgccg tggccgggca cagcgtcggc gagatcgcgg cggcggccgg
     2401 tgccggcgtg atcagcgccg aacaggccat ggtgttcgtc cgcgagcggg gtcgggcgat
     2461 ggccgaggct gcggcggtga ccccgaccgg catgaccgcc gtgctcggcg gtgaccccga
     2521 cgaggtgctg gccaagctgg ccgagcacgg gctgaccccg gccaacgcca acggcgccgg
     2581 ccaggtcgtg gccgccggta cgctcgcgca gctcgccgcg ctcaaggccg atcccccgac
     2641 caaggcccgg ctgatcccgc tgcaggtggc cggcgccttc cacacctcgc acatggctcc
     2701 cgcggtcggg ctgctcgaga gccacgcacg agcgatcagc acccacgacc cgcgcacccg
     2761 gctgatctcc aacgccgacg gcaccgtggt gcacaacggg cgcgacatgc tcgcccggct
     2821 ggtccgccag gtcagcgcgc cggtccgctg ggacctgtgc atgcagacga tggccgacct
     2881 cggcgtcacc gccgtgatcg agatgccgcc ggccggcacg ctgagcaagc tgatcaagcg
     2941 ggcgatcccg ggtgtcgaga cgctggcgct gaagaccccc gacgacctgc cggccgcgcg
     3001 cgagctcgtc gagcgtcacg gcaccgccag cggcctcgac atctccccga cctggcggct
     3061 gctggtctcc cccgcctcgg gcacgttcgt gcgcccgacg aacggcgccg aggtcggcgc
     3121 cacccttgac cacggcagcg cggtcggcgc cgtacgcacc cgtcgcgacg aacgcgtcgt
     3181 cgttgccccg cacggcggca ccgtcgtcga gtggctggtg gaggacggcg acccggtcag
     3241 ccccggccag ccgctggtcc ggctgcaccc gatgtccacg ggcgtctgag acaggaaggc
     3301 acgaccatga ccagccccat caccacctcc gagggtgccc gctacgcgcg cgtcctcggg
     3361 gtcggcggct accgcccggc gcgagtcgtg accaaccacg agatcgtcga caacatcgac
     3421 tcgtccgacc agtggatccg ggagcgttcc ggcatcgtct cccgccggtg ggccgccccc
     3481 gacgagtcgg tcatcgacat gtcggtggcc gccgcgggca aggcgctggc caacgcgggc
     3541 gtcgcgcccg accaggtcgg gttcgtgctg ctggccaccg tcacccaccc ctaccagacc
     3601 ccgtcggccg cggccgaggt cgcgcaccgg atcggggcgg tcaacgccgc ggcgatggac
     3661 atctccgcgg cgtgcgccgg cttctgctac ggcctctcgc tggctaacga catggtccgc
     3721 ggcggcagcg ccggctacgt cgtcgtgatc ggctgcgaga agctctccga cttcaccgac
     3781 cacttcgacc ggtccaccgc gttcatcttc ggcgacggcg ccggcgccgc ggtggtcggc
     3841 ccctctgacg tgccggccat cggccccacc gtatggggcg cgcagggcga gcacgccggg
     3901 ctgatcaccc agaagcactc gtggatcgac gtgcgcgaca aggcggccga gttcccgacc
     3961 ctgaccatgc agggtcagtc ggtgttccgg tgggcggtgt ggcagatggc gcccatcgcc
     4021 cagcaggccc tcgacgcggc cggcgtacgg gctgaagacc tcgatgcgtt catcccgcac
     4081 caggccaaca tgcgcatcat cgactcgatg atcaaggcgc tcaagctgcc ggcgcacgtg
     4141 ccggtggccc gcgacatcgc cgagaccggc aacacctcag ccgcctcgat cccgctcgcg
     4201 atggagcgga tgctcgagag cggcgaggcc aagagcggcg gcaccgcgct gatcatcggc
     4261 ttcggagccg gcctggtcta cgccgcccag gtgctgactc tgccctgacc gtagattgtc
     4321 ccgcgctccc cgcaccccat acctcgcggg cggcaccacc cgttcccaca aagaaggagc
     4381 cccgatggcc cagagcgagc aggagatcct caccggtctc gccgagatcg tcaacgagga
     4441 gaccggcatc cccacgagcg acgtggcgcc ggacaagtcg ttcaccgacg acctcgacat
     4501 cgactcgctg tcgatgatga cgatcgtcgt caacgccgag gacaagttcg gcgtgaagat
     4561 ccccgacgac gaggtcaaga acctcaagac cgtcggtgac gccgtcgcgt tcatccagcg
     4621 cgccggctcc tgagcccgag gagtccgggg gccgccgccg accggcagcg gcccccaggg
     4681 cccccgtacg tcggctctcg cggccccgtc cgaccagcgc cgaccccagt gaccgggagg
     4741 tacccatgag caccaacgag ccgatcgtcg tcaccgggat cggtgcgacg acgccgctgg
     4801 ggggcgacgt cgcctccacc tggtccggtc tgctggccgg ccggtccggc gcccgcacca
     4861 tcaccgagga ctgggtcgag gcgctgccgg tgcagttcgc ctgcccgatc gcggtcgagc
     4921 cggcagaggt cttgtcggtg ccggagcgca agcggctcga ccggtcgggg cagttcgccc
     4981 tgaccgccgc ccgcgaggcc tgggccgacg ccggctcccc cgacgtgccc ggcgagcggc
     5041 tcggcgtcgt cgtcgcctcc ggcatcggcg gcgtgaccac cctgctggcg gcctacgaca
     5101 ccctcaagga gcgcggcgcc cgccgggtgc tgccgatgac ggtgccgatg ctcatgccca
     5161 acgggccggc cgccggggtc gggctcgagc tgggcgcgcg cgccggggtg cacaccccgg
     5221 tcagcgcctg cgccagcggc gccgaggcga tcggctacgg cgtcgacatg atccgctccg
     5281 gccgcgccga cgtcgtggtc gccggcggca ccgaggcggc catccacgcg ctgcccatcg
     5341 ccggcttcgc cgcgatgcag gcactgtcga cccgcaacga cgagccgacc cgggcctccc
     5401 gcccctacga caagggccgc gacggcttcg tgctcggcga gggcgccggc atcgtcgtcc
     5461 tcgagaccct gagcagcgcg cgggcccgcg gcgccaccat ctacggcgag gtcgccggtg
     5521 tcggcctgac cgccgacgcc caccacatcg cccagcccga ccccgagggc ctgggcgcca
     5581 cccgcgcgat gcggctggcc ctcgaggccg ccggggccac cgcccgcgac gtcgtgcaca
     5641 tcaacgcgca cgccacctcg accccggtcg gcgacatcgc cgaggccaag gccctgcacg
     5701 ccgccctcgg tgacgccgtg ggccaggtgg tcgtcacctc gaccaagtcg atgaccgggc
     5761 acctgctcgg cgccgccggc gcgatcgaga cgatcgccac gctgctggcc ctgcgcgagc
     5821 gcaccgtgcc ggccacgatc aacctcgagg accccgacga cgacatcggc ctcgacgtgg
     5881 cccgcaacgg cgcccgggcg ctgcccgccg acggcccgct gctggcgctc aacaactcgt
     5941 tcgggttcgg cgggcacaac atcgccctgg cgctgcggac ggtctcgtga ccaccaccga
     6001 gcacgcgccc acgccggcgc ccgcagctgc gacggcgacc gcagccacgc ccaacccccg
     6061 tgacccgcag ctgcgcctgg gccggttctt cgaccccggc acggtcgagc tgatcacccc
     6121 ggtcgaccgc agcggcatga tcgccgccac cggcctggtc gagggcagcc cggtcaccgc
     6181 gttcgcctcc gacgccaccg tgatgggcgg cgcgatgggc gtcgacggct gtcgggtgat
     6241 cgtgaccgcc tacgagcacg cgctcgagac gggcaccccg gtcgtcgggc tctggcactc
     6301 cggcggcgcc cggctgcccg agggcgtggt ctccctgcac gcggtggggg aggtcttcgc
     6361 gatcatgacc cgggcctcgg gccggatccc gcagatctcg gtggtgctcg gcccggccgc
     6421 gggcggcgcg gcctatggcc cggcgctgac cgacgtggtg atcctcggcc cggacggacg
     6481 catcttcgtc accggccccg atgtggtgcg ctcggtgacc ggcgagaacg tcgacgccct
     6541 gcgcctgggt gggcccgagc cgcacggccg gcgctccggg gtggtccacg tcgtcgcccc
     6601 caccgacgac gatgcctatg acgaggcccg ccggctggcc gccctgctcg gcgaccaggg
     6661 ccagctcgac gccgcaacca tcgaggacgt cgacctgggc gagcacctgc ccgagtcccc
     6721 caagagggcc tacgacgtgc atccgctcat cgacgggctg ctcgaccggc ccgggctcga
     6781 gctgcacccg cggtgggcgc ccaacgtcgt caccaccctc ggccgcttcg gcgggcgcac
     6841 cgtcggggtg atcgccaaca acccgttgcg gctgggcggc tgcctcgact cctcctcggc
     6901 cgagaaggcc gcgcggttcg tgcgcatgtg cgacgcgttc ggcgtaccgc tcgtcgtgat
     6961 cgtcgacgtg cccggctacc tgcccggcgt cggccaggag tgggacggcg tcgtacgacg
     7021 tggcgccaag ctgttgcacg cgttcgccga ggcgtcggtg ccgcgcgtca cgctggtcac
     7081 ccataaggcc tatggcggcg cctacatcgc gatgaactcg cgctcgctgg gcgccaccaa
     7141 ggtgttcgcc tggccgggcg ccgaggtcgc cgtgatgggc gcggtggccg ccgtacgcat
     7201 cctgcaccgg cgccggctgg ccgaggtgcc cgaggagagc cgcgccgacg tcgagctcga
     7261 gctggccgcc gagcacgaga agctggccgg cgggctggag cgggcggtgg agatcggcgt
     7321 cgtggacgag gtggtcgagc ccaagctcac ccgctcgacc ctggcccggg ccatcgcggc
     7381 ggtgccggcc acccgtggcc agcacgccaa catcccgctc tgaccggctg accggcttca
     7441 gaggaacagg gcgccacccc ggctagacca cctggtgcag ccagcggacc ggggcgccgt
     7501 cgcctgcgta gcggaacggc tcgagctcct cgtcccaggg gcgacccagc agccggtcga
     7561 cctcctcggt gaggtcggcc tccccggcgg cggcccggac catggccgcg cgcagccggt
     7621 cctcgggcac cagcaggtcg ccgtgcaccc ccgtcacggc gtggaagatg cccaggtcgg
     7681 gcgtgcacga gtagcgctct ccctcgcaac ccggcgaggg ctcctcggtg acctcgtagc
     7741 gcagccgagc ccacccccgc agcgcagacg cgatcttgga acccgtggag ggactcccct
     7801 gccaggacag ctcggcgcgc agcagccctg gcgcggcggg ctggggggtc cagtcgaggg
     7861 agacccgcac gccgagcacc ccggcgatcg cccactcgac gtgaggggcc agcgccggcg
     7921 gggcggagtg gacatagacc acgccgcgcg cggacggctg cgggtgagca gcagacatca
     7981 ccggacctcc tagcaggctg cgagatgcgc cttccccagc ggtctcagca gtgctcggct
     8041 gcccgttgcg cgcaccattg tgccgtatcc ggcccgacct gcaccagcaa ccggagtctt
     8101 gtccaaaatg agattgaaga tcttctcaac tcgcgccgat agaccatctg ggttacacaa
     8161 tgaccacgcc gcgggggacg gggcaccaca actggaggat gcacatgact gacgccgagc
     8221 tgctcatggc ggcggcgtac gacgaggggg tggtcagcga ggtcatccga ccggccgcga
     8281 tcatccccga ggaggccgcc cgggcagtgc tcgtcgagct tgcgctgcgc gacgtgcaga
     8341 acggcgggaa ctggcagtcc gaaccctcgt tgtggagccg gttcgaccgg cccttccacg
     8401 gaccgaacaa cccggccggc gccgagctga tcggcaccat ccaggtcgcc tacggcaccc
     8461 cgacccgcta cgagatcacg atctaccggg ccaccgtcac tcgactcggc accgagaacg
     8521 gctggaccgt cgaggcgctg tgcgacgagg cactggcgtt tggcaacctg agcctggcga
     8581 cctgcccgcg ggccaagctg gccgcgccgc ccaagccctt tcgcttctag ttccaggcct
     8641 cttctagacc ggcctcgctc acgcctgcgg cggggccagc agcgcggtga gccgctcgag
     8701 cagcgggcgc tgcccgacca gcagggcgtc atgggccgcg ggcaacggga accacgcgca
     8761 ccggtcgagc tcgggcacca gcatccgacg ccccgaccgg ggcggccact cgatctccac
     8821 ggtgttgctc accatcgtcg ccgggtcgag gtcaccctcg acggcccagg cgagcacccg
     8881 tttgccgcct gactgacgca cctcgcccag cggcaccggc tcggcgtccg gcaccggctg
     8941 gccgagctcc tcgacgaact cgcgccgggc cgcgaccagc gggtcctcgg tggcgtcgta
     9001 ctcacccttg gggatggtcc aggcgccggc atccttgcgt gaccagaagg gcccgcccat
     9061 gtgcccgagc agcacctcga cctcaccggc ctggcgacgc cagagcagca ggccggcgct
     9121 gcggcgcccg gaggtcgcgc tcatcggctg acgacccaga ccacgtcggg cccgccgcgg
     9181 gccacgtcga ctcgatgggt gcgcacctgt gcgaagtgct gacccagccg ggcctcgaag
     9241 gtctcagacc cggtcgcgct ccagatgccg gccaccccgc ccgggcgcag cgcaccggcc
     9301 agcgcccgga ggccggcgtc gtcgtagagc gcggcgttgt ccgggcgaac cagccagtcc
     9361 ggcccgttgt cgacgtcgag gcagatcacg tcgtacgacg catgcgcctg cgccagccag
     9421 gcggtgatgt cggcgcagac cacctgcacc cgcgggtcgt cgagggcgcc cgcggtgacc
     9481 gcggcgagcg gaccgcggtg ccactcgacc agcaccggct cgagctcgac gacgaccacg
     9541 cggccgacgg tggggtgggc cagcacctcg agcagggaga agccgacccc gaggccgccg
     9601 acgaccacct cgagggggcc tgacggctcg ggcgcggcgg ccaggctggc ctcgacgagc
     9661 aaccgctcgg agcgcccgtc acgggtgtcc atgaggaaga cgccgttggc gaccacctcg
     9721 aagtgctcac cgtgccggcg caggacgagc tcgcctgaga gcccggccgc gcgggcgagc
     9781 acggtcgggg cgctggggcg cgccggcacc cccgcccgct caggcgccgt cggctccctc
     9841 ggcggccggg tgggcggcga ccagcggggt gtcgacgccg cgggggccca gcttggcggc
     9901 gccgccgggc gagccgagcg gctcgtcgtg gccgggcagg gtctcgctga agaaggcggc
     9961 gttgtcttcc ttgtagagct cgaacccgcc gggcaggtcg tcctcgtagt agatggccgt
    10021 caccgggcag accggctcgc aggccccaca gtccacgcac tcgtcggggt ggatgtagag
    10081 cgagcggccg ccctcgtaga tgcagtcgac cgggcactcg tcgacgcagg cgcggtccat
    10141 cacgtcgatg cacggctcgg cgatcacgta ggtcatgcgg tccttctctc gttcgatcgt
    10201 gctacccgct gggctcgcac cggccagcct ggcacaggtt gtgatttatg caccataggt
    10261 gttgcgtaaa taacgccatc cgctcatcat ggccgcggga ggcaccgatg agcgtcgtca
    10321 tctacgggga cttcacctgc ccctactcct atctggccag tcgtcgcact gatgctctca
    10381 tcgaggccgg cgtcgacgtc gactggcacg cggtggagcg ccgcccggac ctgccggtca
    10441 ccggcgtcgc actcacgccc gactcgcggg ccgcgctcga ggcggaggtc gccgaggtca
    10501 ccgagctgct gcgccccggc gaagagctgc cgctcgagat ccacggcacg atcccccgca
    10561 cggaggcggc ggtgtccggc tacgcggagg cctacggcgt ggggaccgcg gccgacgtac
    10621 gacgactgct cttccgcacc tactgggtcg agggcggcgc gatcggcgac ccggaggtgc
    10681 tgcgccagct gctggtcggg ccgatcctgc gcggtgacgg cggcgccggc ccgttgcacg
    10741 agttcggcta tgccgtcgcc gtgagccggg ggccgatgac caccgcggcc tttcggcaca
    10801 tccaggcctg gcgggccgga tgggtggcca ccggcaccgg caccactccc accgtggtcg
    10861 tcgacggcac cgtccatgcg ggcgtggcgg ggctgcactg gctggccgat gagatcgacc
    10921 ggctcggcgc gccgcgcgca ccggagccgg ccgaacccag ccgctatccc gaggtgagcc
    10981 tccgtcccca gccggagtgg gtctcgaccg tcggtgggcc ctgggcgcgc gccttcatgc
    11041 accgctgacc ggcgaggagt caggcagtct cgagcaccgc ggtgaccgcg atcttggcgc
    11101 cggcgaacag ccgcgagatc gggcacagcg cggccgcctc gtcgaccgcc gcctggaacg
    11161 tcgtgacgtc ggcacccggg accaccgcgc gcacgtcgac atccgaggag accacggtgg
    11221 gcagcccgtc gacggcgtcg agggcgacgg tcgccgtcac gctcagccgg gtcggcgtca
    11281 gcccgtgctc gccgagccgc agcgagaggg ccatcgagaa gcacgacgag tgcgccgccg
    11341 cggccagctc ctccgggctg gtcttgccac ccggcgcctc ggtgcgcgag gcccaggtca
    11401 ccggcagctc gccgtaggcc ccgctgttgc cggtgatcac gcccgccccg ctggccagcg
    11461 ggccctccca cacggtctgc gcactgcgct ctgcgatagc cactgctgtc tcctcatcgg
    11521 ttgtgagccg gcccccgttg ccgaacgggc ggcctttccc ccggattctg acaaaccggt
    11581 gtgggcaata tcgctggggg tcctccccat gtttgaggct catgcccggt ctcacgccaa
    11641 gaacgactat gctgtggtcg ctaacggcgc tgacgaagcc cattcgcgcc gtattcgata
    11701 tcccaccaac ttcgagcgtg aggaccctca tggcgcaaaa gaccatcgtc cagctggtgg
    11761 acgacctgga cggcaaagag ctgacggccc aacaagggca aactgtcacc tttggcctcg
    11821 acggggtcag ctatgagatc gacctgagca acaagaacgc caaggcgctg cgcgacctcc
    11881 tcggccgcta cgccgacgtg gcccgcaagg tgggcaccgc gcgcgcgggc tcgggccgca
    11941 tcaagcggac ccagctgggc ccggatgccg tcgtggtcaa ggcctgggcc gacagcaagg
    12001 gtctcgacta cccgaagcgt ggccggctgc cgaaggcgct gctggagcag tacgccgccg
    12061 accaccgcta attctgctcg tcgtggcccg gccacggcgg gcagtgtcgg ctgattacgc
    12121 cgacggccac aaatctggct ggctagagtc gaagtgcgct cgcaccactc gcacgccgca
    12181 gtgaacgatc atccgatctg gtcactgccc agccgggagg acaagaccgt ggccgtgaaa
    12241 tcgccgaagt cttcgcagac gagccagccg tggctgcacc agaagggtgc gcagatccag
    12301 aagttcggga cggttcgtca gctgcccatc ggcctgagcc aggagacccg cgcctactcg
    12361 tgcgagcgtc tcaacgcgtg cctggccgac tcgcagatcc tttatggcct ctacaagaag
    12421 caccactggt tgatgcgcgg gccgacgttc taccagctgc atctgctgct cgacaagcac
    12481 gccggcgagc agctgctgct catcgacagc atcgcggagc gggtgcagac cctgggtggc
    12541 gtggcgatcg gtgacccgcg ccacgtcgcc gagatcacca ccgtgccgcg cccgcccgac
    12601 ggggtcgaag aggtgccggc gatgctgtcc cggctgctgg aggcccacga gatcatcctc
    12661 gagcaggccc acgacgcggc tgctcgggcc gacaagatgg gcgacgacgg caccaacgac
    12721 ctgctcgtgt ctgacgtgat caggaccaac gagctgcagg cctggttcct tgccgagcac
    12781 ctggtggaca cgccactcgt gcacgcctga ccgccggccg gccgtgcggt gacgcccgca
    12841 ccggcgagtg attcgcggtg ctctcacgcg gtgctgtcga tctgcaggga cgcgatgccg
    12901 gtcatctcca gccaggtgcg attgccggct acgaccagcc gcgccagcac gtgctcgcac
    12961 gaggagcagc gcagtaccag cccaggtccg gcggagtaga cccgacactg cgccatcacg
    13021 gcgctctcac cgcagccggc gcaccggccg accgcgacgg tgatgtcgaa ccccagcacg
    13081 tcggccatcg gaccagccgc ggcgttgcca tcgaggaatg tcggtgacgt gtccatgcag
    13141 gctcctcacg gcccgaaacg ttcggccctg atgttgctag ccgggaatcc cgctgcctcc
    13201 agcagcgtga tcccggtctc gacgaagccg gttggcccgc acacgtagca acgagtccgc
    13261 ggcgagggcg gcagggcgta cgccgcgatg tcggaggcgc tgagccggcc gggcggcctg
    13321 gccgcgtcgt cgggcgcgtc tcgcgtgtag aggacgctca ccgtcgacga cggtgcgcca
    13381 tgcgcgaccc gccgggtgag ctcggcgccg tagagcagcg cctcgggact tcgagtcgag
    13441 tagagcaggc gcatcgggtg cgggtcgggc acctggtcgt gctcgcgaag catcgcggtg
    13501 agggggacga cgccgacccc gccgcccagc agctgcaccg gcccggggtc ttcccggtcc
    13561 cagacgaacc accctcccag cggacccctc acctccagct cggtgccgat ccgggcgccg
    13621 cgcaccagat agctcgagac ctcgccgtcc tcgacgaggg ccacggtgat gtcgatgcgc
    13681 gagccgtcgg tcggcctcgc gagggagtag gggcgcaccg cggtgtagcc atcctgggcg
    13741 gtcaggcgga ggtcgacatg ctggcccggg cggtggccag gccagccgtc gaccgtgagc
    13801 gccaggctgc gagcggtgga ggtctcctca cggatggccg ccactgtcgc cggacgccag
    13861 ctcagtcgcc ccactgacgc tgctcccgcc acgggtcgcc gtactcgtgg tagccgagct
    13921 gttcccagaa ccccggctcg tcctcgtcct gcacccgcag cccccgcagc cacttcgcgc
    13981 tcttccacag atacaggtgc gggacgagca ggcgcgccgg cccgccgtgc tcggggtcga
    14041 gcggctctcc gtcgtagtcg aaggccaccc acgccttgcc gtccagcagg tcctccaacg
    14101 gcaggttggt ggtgtatccg ccgtacgacg aggccaggac aaagctgccg tcgacgtcgg
    14161 cctcgtccag cagcgtctcg accgggacgc cggtccaggt ggtgccgagc ttgctccacc
    14221 gggtcacgca gtgcaggtcg acggtgacct cctcggcggg cagggcacgg aactcgtccc
    14281 agctccagct cgctgccaca ccctcctctc cgctgacctc gagctgccag ctctcgagcg
    14341 gcacgcgcgg cgtggcgccg gccgagagca ccggaaagtc gtgggtgagg tactggccgg
    14401 ggggcagctc ggtgccgccg gcgcgccggt tgcgccggcc catgaagcct tccgagaagc
    14461 tggacatgac agatcctcca ggtcggtctg cgcgtcctgg ccggggagga gcgaagccgc
    14521 ggacgagatc accgatcgga cctgcgtcgc agaccatgct cacccatggt gctgcacgct
    14581 ccgggctgcc gggtgctcgt tggggcagtc ggcgctatgt aagcgcgctt gcgaactttg
    14641 atggtggcga gttcagacga tgatggcgac cttgccccgg acttggccgg acgccaggtg
    14701 gcgcatggcg tcggggaccc ggtccagggg gtaggtccgg tcgatgctag gcgtcactcg
    14761 gccggcttcg atgagctcgg tcagccgctc gaggtcgctg gcccgctcct tggcaacgaa
    14821 ccagaccagc cgctgaccca ggaacggcga gagcaccagc gcacgcagcg gccggttcat
    14881 cccgccggtg aggttgccgc cgctctcgcc gccgacgagc acggccgtcc ccttcggggt
    14941 gagcgctcgc cgcagccggg acagcctcgg gttccctgcg atgtcgagga ccaggtcgta
    15001 gcgagccgtg ccgttggcgt agtcgtcgct ggtgtagtcg agcacccggt cggcgccgag
    15061 cgagcgcacg agatccagct tcgccgtgct ggccacaccc gtgacctcgg cgcccaacgc
    15121 cttggccagc tgtacggcgt agctgcccac tcccccggac gcgccgatga ccagcacgcg
    15181 ctgacccgcc tcgacccggc cgtggtcggt cagtgcctgg agcgcggtcc cggccgagat
    15241 cggtacgacg gcggcctgct cgaacgacag gttcaccggc ttgcgggcga gcttgtcctg
    15301 cttcgcggcg gcgtactccg cgaacgagcc gcggccgacg ccatatacct cgtcgccgac
    15361 ttcgaacctg gtcaccgcgg acccgacggc ctcgaccgtg ccggcgaggt cacgccccga
    15421 cacccggtcc ctaggaccgc ggacgccgag gcccgcgatc cgcatcagat agggcctgcc
    15481 cgtcatcaag tgccaggtgc cgcggtccag acccgccgcg tgcacccgaa ccagcacctc
    15541 attgtcagcc acttcggggc gggcgatgcg ctcgtggcgc agcaaatcgg cgtcgtcgcc
    15601 gtacttgtcc tgaacgattg cccacatcgt ggccatcgtc gggtccttgg gagatgtcgg
    15661 tcgaaggctg acgaacggcc gcgtggcgat gaactcccgc atagccgcct ccgagcgcag
    15721 gtcatcccag ccgagcaccc ggctcagcaa ccggagccgc ctcgggtggc ggcgccggaa
    15781 ctccaccgcc actgcgaaag cctcgtctga ggtgaggaat cgctgctccg ggacgaatga
    15841 cgcccggccg atctggacgc gcagggccgg gtgagcgcga aggttctgga cccagtcggc
    15901 gcgcgcgccc cacaccgagc agatgaccac ctcgccggtg ctcttgtcgc gggcgatcac
    15961 catcgcggcc gtggcgtgcg gcttcccggt ctttcggccc acgtgcacca acaacaagaa
    16021 ggtgtgcccc agcagccatc ccaagcccat ccggtacaac ggcagcggca accggaagaa
    16081 cgacaaggcg agcctgcccg gctgccggcg gaaccccagc aagggcctgc ttcgcgttcg
    16141 ggtccgttcc ggcgctgacc cggatgtcac gtggctcacc tccagccacc ccgccgctct
    16201 cagcatcaga caaccgcgcg cggccatcct gcgtcagagg cgtaggtcct tcggcctccc
    16261 tcgtccgccg ccggagcgcg cgcatgcagc gtgggacctt cgtcgtcgcc ggctgggtga
    16321 ttcggctcta ctccagtcat gccgtgaggc gaagggttag gtacgtgacc aggagtgtgg
    16381 agagccggag tgcggcaggc cgcgccgatg gttcgatggc gatcttgacc cgcgggctgg
    16441 tgaaggactt cggcaggacc cgagcgttgg accacctcga cctggcgatt tcggccggcg
    16501 aggttcatgg tttcctcggg cccaatggcg ccggcaagac caccacgatc cggattttgt
    16561 tgggcctgtt gcgcaggacc accggcgacg tcatgctcct tggcggcgac ccgtggcgcg
    16621 acaccgcgac gcttcatcgc aggctcgcgt atgtgcccgg cgagatgaac ctgtggccca
    16681 acctcaccgg cggcgaggtg atcgacctgc tgagctcact gcgtggcggg ctcgtgccgg
    16741 cacggcgcgc ggctctgctc gagcagttcg acctcgaccc caccaagaag tgccgcactt
    16801 actccaaggg caaccggcag aaggttgcgc tagtggcggc cttctcctcc gacgtggagc
    16861 tctacctcct tgacgagccc acgtccggac tggacccgct gatggagacg cagttccagg
    16921 ccgtcgtgcg cggactgcgc gccaacggca agaccatcct gctctcgagc cacatcctct
    16981 cggaggtgga ggcactgtgt gaccgggtca ccatcatccg cgacggccat gtcgccgaga
    17041 gcgggacctt cgccgagctc aggcatctga cacgtacgac tgtttcgatc gagaccaccc
    17101 gcccggtggc aggcctggcc gatctcgacg gtgtccacga cctgtccgtc gacttcaacc
    17161 gggcccggtt cagcgtcgat gccgacgacc tcaaccgggt gctcgagcac ctggtcgcct
    17221 acgaggtgcg gtcgatgacc agttcgccac ccacgctcga agagctcttc ctgcgccact
    17281 acgacacccg cggcgactcc gaccgcgcac cggccggcga ggtggtgctc gcccgatgaa
    17341 cgcgatgtcg ggaacgatcc ggctaacccg gctcgcagca cggcgggacc ggatcacgat
    17401 tccggtctgg atcctggggc tggccgcctt cctgaccgcg accacggcca tgttcgtgaa
    17461 ctcgctgatc acccacgagg acgtggtgcg tgaggccgag ctccctacga acaacgtcgg
    17521 cctgcggttg ctcggcctca cttcgggccc gaccgtgggc ggcgcggtga tggtgcgtga
    17581 ctacgtctgg ctcgcggtga tggcagcgct gatgagcgtg ctcgcggtgg tccggcacac
    17641 ccgccagagc gaggagctcg gccgggccga ggtcgtcggg tccacggtcg tcggccggta
    17701 cgccgatttg gccgcggccg tcatcatcgc cgtggccgcc aacatggtgc tggccgtgtc
    17761 gctcgcgctg gcgatggtcg tcaacggaca gccggcgtcg ggtgctttca ccgccggcgc
    17821 tggtgttgcc gcggtcgggg tggtcttcac gggcgtggcg gcggtgacgg tgcagctcgc
    17881 gtcgacgacg cgtggcgcca tcggcatggc cggcgcggtc ctcggtgtgg cgttcctgct
    17941 cagcggcatc ggcaacatgc tcggcaccgc ggacacccgc gccctgcggg tcaccagtgc
    18001 ctggcccgcg tggctctccc cgatcggatg ggctcagcag atgcgcccgt tcggtgggaa
    18061 ccactggtgg ccgttggcac tcttctcggt cctgttcgcg tgcctgctcg tcaccgctcg
    18121 cgcgctcgtg gtgcgccgcg acgtcggagc tggactttgg ccgcagcgtc gtggccacgc
    18181 caccgcggcg gcgagcttgc taagcccgac cgggctcggc ttccgattgc agcgtggtgc
    18241 gttgctgggc tgggcggtcg gcatgctcgc cttcgggctg atcttcggcg ccatgtccga
    18301 gcagatccag gacgtcaccg gccaggcccg ggagttctac gaacgcttcg gcgggaccga
    18361 caagatcctc gagtcctacg acgcctcgat ggccgcgatg ggtgccatgg tcgtggccat
    18421 ctacgtggtc cagatggtgc tgcggatgcg ggccgaggag gccgcgggat cactcgagtc
    18481 gctgctcgcc tccggcgtca cccggccacg ctggttcctg ggccacctgc tgaacaccgt
    18541 cggcggcgca gtcgccttga ttctcctcta ctcagccggg atgggcatcg gcgcaggcca
    18601 ggttctgggc ggcaccccga cccagctggg caagacgctc accgccgggg tcgtgcaagt
    18661 gcccgcgatc ctcctgattg ggggctgtgc cctagtcgcc gtcgccgtgc tgccccggct
    18721 cgcctcgcca ctggcgtggt cggtggtgat cgctgcgctt ctgctcgggc caatgttcgg
    18781 cccggggctc ggtgtgccgg agggggtcat ggacctgtca ccgttcaccc acgtgccgac
    18841 cgtgcccgcc accgacgcct ccgcgccgcc gctgctcatc ctgaccggcc tttgcgtcgc
    18901 tttcggcgtc atcgcggccg ccgccatgcg cagacgcagc ctgctgctcc ctgcctgagt
    18961 cgccatgtcc gtggcaatgc gctggacgcc acgcaacagg aaggagggat cacggggtga
    19021 cgccctccaa cgtggtcgtc cggtggcgga tgaacaactt ggcgccgcgg accaggtccg
    19081 tggacctact gcgccaccgg ctggtggtgg tgacctcggt gtccgagatg ctcgggcacg
    19141 cctcggccgc gatgaccctc gacgtctacg ccggcctgtt cggcgacgac ctcgacgcgg
    19201 tcgccgaccg gctcgacgcg gcagcccggc gcgcggctgc ggactttttg cggactagcg
    19261 cccctaccgg ccccgaatca cctgacggtg attcacccgc cgcacccccg ctgacctgcg
    19321 cagatgcgcg gtggggcggg tggggctcga acccacgacc cagggattat gagtcccttg
    19381 ctctaaccgg ctgagctacc gccccgagca ccgctcagcg acgcggtctc gtcgagggca
    19441 cacgttacgc gacgacgctc acgcga
//
LOCUS       DATIBB010000085         2253 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_1753699, whole genome shotgun sequence.
ACCESSION   DATIBB010000085 DATIBB010000000
VERSION     DATIBB010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2253)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2253)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2253
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1753699"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..274
                     /locus_tag="VLC50_03085"
     CDS             <1..274
                     /locus_tag="VLC50_03085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF26480.1"
                     /translation="QVLIRDADGHVVARADFLWRAQRVVGEVDGKVKYVGPRADSLWR
                     EKRRQEAVEDAGFVVVRWGAFELATPAALDQRIRRAFEQAARRRVA"
     gene            complement(278..1216)
                     /gene="metF"
                     /locus_tag="VLC50_03090"
     CDS             complement(278..1216)
                     /gene="metF"
                     /locus_tag="VLC50_03090"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020573411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HSF26481.1"
                     /translation="MALGLPSTMPGGATTIRELLAGGDRSFSFELFPPKTEQGERILW
                     RALRELEALRPTFVSMTYGAGGSTRERTVRITGEIAEHTTLTPVGHLTCVGVSRHSLR
                     SVIGAYADVGVRNILALRGDPPGGVGGAWVSHPDGLAHADELVALVHSLGDFCVGAAA
                     FPDGHPEAVDLDADARVLGRKAELGADFAITQFFFDVDRYSALVDRLARLGCDIPVIP
                     GIMPVTDVRQIVRFAQLSGTALPPDLLARLDAVRDDPVAVRAVGVEHATVLCRRLLEH
                     GAPGLHFYTLNRSTATREVYADLGLAGVAVGAGSAR"
     gene            1259..>2253
                     /locus_tag="VLC50_03095"
     CDS             1259..>2253
                     /locus_tag="VLC50_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012087051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HSF26482.1"
                     /translation="MPATTPDAAADLRERVQTRLDSFLARKAGVLAEVGDELVALLDA
                     ATDLLRGGKRLRPAFCYWGWRGAGGTDTGAEGEAIIAAAASLELFQAAALVHDDLMDD
                     SDTRRGQPAAHRRFAALHRDQGWAGEPAAFGLAGAVLLGDLLLSWSDELLMTSGLALE
                     RLADARVVFDTMRTQLMGGQYLDILAQVSGAESVQRSRHVIRFKSAKYSVEAPLLLGG
                     RLACASDALLTSYSAYGLAVGEAFQLRDDLLGVFGDPAETGKPAGDDLREGKRTVLVA
                     TALERASAQQASVVRRLLGDPGLSGAGVDALREVLVDTGARDEVEAMIEQRSREAL"
BASE COUNT          313 a          787 c          858 g          295 t
ORIGIN      
        1 gcaggtgctg atccgcgatg cggacggcca tgtggtggct cgggccgact tcctctggcg
       61 ggctcagcgc gtcgtcggcg aggtcgacgg gaaggtcaag tacgtcggtc cccgcgctga
      121 ctccttgtgg cgggagaagc gccggcaaga ggcggtcgag gacgccggct tcgtcgtcgt
      181 gcggtggggg gctttcgagc tggcgacgcc ggcggcgctc gaccagcgga tccggcgcgc
      241 gtttgagcag gcggcccgac gtcgggtggc ctgacgatca gcgggcggag ccggcgccga
      301 ccgcgacgcc ggccaggccg aggtcggcgt agacctcgcg ggtggccgtc gaccggttga
      361 gcgtgtagaa gtgcagcccc ggcgcgccgt gctcgagcag ccggcggcac agcaccgtgg
      421 cgtgctcgac gccgaccgcc cgcaccgcga ccgggtcgtc gcgcacggca tccaaccggg
      481 ccagcaggtc gggcggcagc gcggtgccgg acagctgggc gaaccgcacg atctggcgga
      541 cgtcggtcac cggcatgatc cccgggatca ccgggatgtc gcagcccagc cgggcaagcc
      601 ggtcgaccag cgcgctgtag cggtcgacgt cgaagaaaaa ctgcgtgatc gcgaagtcgg
      661 cgcccagctc ggccttgcga cccagcaccc gcgcgtccgc gtccagatcg accgcctcgg
      721 ggtgcccgtc ggggaaggcc gccgccccga cgcagaagtc accgagcgag tgcaccagcg
      781 cgaccagctc gtcggcgtgt gcgagcccgt cggggtgcga cacccacgcg ccgccgacgc
      841 cgccgggcgg atcgccgcgc agggcaagga tgttgcgtac gccgacatcg gcatacgcgc
      901 cgatgaccga gcgcagcgaa tgccgggaca cccctacgca ggtgaggtgc ccgaccgggg
      961 tcagcgtggt gtgctcggcg atctcgccgg tgatccgcac cgtacgctcg cgggtggagc
     1021 cgcctgcgcc gtacgtcatc gagacgaacg tgggccgcag cgcctcgagc tcgcgcaagg
     1081 cccgccagag gatgcgctcg ccctgctcag tcttgggcgg gaacagctcg aaggagaacg
     1141 accggtcgcc gccggccagc agctcccgga tcgtggtggc accaccgggc atcgtcgatg
     1201 gcagacccag tgccatgcgg tcactgtagg aacgagccgg ccgcctaggc tgccagtcat
     1261 gccagcgacc acgcccgacg ctgccgcaga cctacgcgaa cgggttcaga cccgcctcga
     1321 ctccttcctg gcccgcaagg ccggggtgct ggccgaggtc ggtgacgagc tggtcgcgct
     1381 cctggacgcc gcgaccgacc tgctacgcgg cggcaagcgg ctgcggcctg ccttctgcta
     1441 ttggggctgg cgcggggccg gtggcacgga caccggcgcc gagggcgagg cgatcatcgc
     1501 ggcggcggcc tctctcgagc tgttccaggc ggccgcgctg gtgcacgacg acctcatgga
     1561 cgacagcgac acccggcgcg gccaaccggc ggcgcaccgc aggttcgccg cgctgcaccg
     1621 ggaccagggc tgggccggcg agcctgccgc gttcgggctg gccggggcgg tgctgctcgg
     1681 cgacctgctg ctgtcgtggt cggacgagtt gctgatgacc agcgggctgg cgctcgagcg
     1741 gctggccgac gcccgggtgg tcttcgacac gatgcgcacc cagctgatgg gcgggcagta
     1801 cctcgacatc ctcgcccagg tgagcggcgc cgagtcggtg cagcggtctc ggcacgtaat
     1861 ccggttcaag agcgcgaagt attccgtcga ggcaccgctg ctgctgggcg ggcggctcgc
     1921 gtgcgcctcc gacgcgctgc tcacctccta ctccgcctat gggctggcgg tcggcgaggc
     1981 gttccagctg cgcgacgacc tgcttggcgt gttcggcgac ccggccgaga ccggcaagcc
     2041 ggccggcgac gacctgcgcg agggcaagcg cacggtgctg gtcgcgaccg ccctggagcg
     2101 cgcgagcgcc caacaggctt ccgtcgtacg acgactgctg ggtgaccccg gcctgtcggg
     2161 tgccggcgtc gacgcgctgc gcgaggtgct cgtcgacacc ggcgcgcggg acgaggtcga
     2221 ggcgatgatc gagcagcgct cccgggaggc cct
//
LOCUS       DATIBB010000086        12690 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_1227306, whole genome shotgun sequence.
ACCESSION   DATIBB010000086 DATIBB010000000
VERSION     DATIBB010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 12690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12690
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1227306"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            131..1057
                     /locus_tag="VLC50_03100"
     CDS             131..1057
                     /locus_tag="VLC50_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018784650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSF26483.1"
                     /translation="MGAYIARRILQMIPVVLGATFLIYSMVWYLPSDPFAGRCGDRPC
                     PEAYVALMREKFNLDDPLFVSYFKYFGDLLTGDFGETFQGLQVRDEILRAYPVTMKLA
                     LIAILIEIFIGIGAGVLAGLRRGKFIDNLVLVSTLVVISIPVFVIGTTLQFWLGIKFP
                     IFPPTVSSEATWYSLLLPGFVLASLSLAYVARLVRTSIGENIRADYVRTAVAKGLPGR
                     RVVGLHVMRNSLIPVITFIGADFGALLGGAIVTEGIFNIAGVGGLAFRAIRTQEAMTV
                     TGVVTVLILVFLIVNLLVDLLYGVLDPRIRHD"
     gene            1050..1985
                     /locus_tag="VLC50_03105"
     CDS             1050..1985
                     /locus_tag="VLC50_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019874393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSF26484.1"
                     /translation="MTSTVNAPSTSPTEAEPALGKPRSLGSEAWLVLRGRAMFWIALV
                     LIAVIVLMAAFPGLFTSVDPYAGDLSRSRQAPSLDSQASWFGYDSVGQSVYARVVYGA
                     RASILVGIITTVVVTLVGGFLGTVAAFYGRRTDTLISRVGDIFFGIPLLLGALVVLVS
                     FPSDENTPSWATISKVVFALAVLGWPGIMRIMRSSVLQVKSGDYVMAARALGASGSRI
                     IRKHVVPNAVAPVIVYATISLGVYITIEATLSFLGIGLQPPVVSWGVMISDAQNYLRV
                     APHMLLFPSAFLSVTVLAFIMLGDVVRDALDPKLH"
     gene            1998..3032
                     /locus_tag="VLC50_03110"
     CDS             1998..3032
                     /locus_tag="VLC50_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015779235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSF26485.1"
                     /translation="MATQAVTLPSGASLPDTSPLLEVRDLRVEFRTREGVAKAVNGVS
                     YTVDEGETLAVLGESGSGKSVSAQAIMGIIDSPPGFVTGGQVLFRGDDLLTMPEDERR
                     AYRGRKIAMIFQDALSSLNPVFPVGWQIAEMFRVHEHLSRKEARARAIELMERVRIPA
                     AAERVDDYPHHFSGGMRQRIMIAMALALNPDLLIADEPTTALDVTVQAQIMELLADLQ
                     RESNMGLILITHDLGVVADVADKIAVMYAGRIVEQAPVYDIYANPAHPYTEGLLQSIP
                     RVGQKGQKLSAIEGLPPNLTRIPPGCAYHPRCPYARDICRTENPALRTVGPDRVSACH
                     FAEEVLDGGR"
     gene            3019..4047
                     /locus_tag="VLC50_03115"
     CDS             3019..4047
                     /locus_tag="VLC50_03115"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018381816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HSF26486.1"
                     /translation="MAAASLSREVVLDAQDLVKHFPLTTGVVIKRRVGAVQAVDGVSF
                     QLHKGETLGVVGESGCGKSTLAKLLMRLEEPTSGHAYFGGKDIYSLRGRALRDMRRRV
                     QIVFQDPYSSLNPRMTVGDIVGEPFEIHPEVAPKGERRKRVQELLDVVGLNPEHINRY
                     PHQFSGGQRQRIGIARGLALRPDVIICDEPVSALDVSVQAQVINLLEDLQSEFGLSYI
                     FIAHDLSVVRHISDRVAVMYLGKIVEIGDEDQIYSRPAHPYTQALLSAVPVPDPTLRG
                     RREQIVLEGDVPSPANPPSGCRFRTRCWKAQEICASEEPALVRRPGVDHESACHFAEV
                     RQIVPASD"
     gene            complement(4072..6204)
                     /locus_tag="VLC50_03120"
     CDS             complement(4072..6204)
                     /locus_tag="VLC50_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006132007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HSF26487.1"
                     /translation="MTETDLPMSYPRLKARTLSFTLGVPREFTISSDRSRVAFLRSRG
                     GTDRAACLWVADIVEEGLLERVVADPHELLDGGQERLSPAERARRERAREGGAGIISY
                     ATDEAGTRAAFALSGRLFVTDLLTGQCRDLDVTGPVIDPRPDPTGEWVAFVADGALRV
                     VPFTGGPERVLAAPDGEQITWGLAEFVAAEEMDRDRGYWWAPDGRSLLVQRTDESPVT
                     VWHIADPAHPALPPASVRYPAAGGDDAEVTLWLVDLDGARREVSWDRTALPYLTRVSW
                     TSYGDPLVQVMSRDQRRAVVLAVDAASATSYQVHEASDDTWLDLVAGVPCWTPKGALL
                     TTVDADGCRRLAADGVAFSPVGVQVRAVAGVDGTGALVTASTEPTEIVLARIGWDGEV
                     TGLAESPGVHTGLVRDDVAIVSSRTLEHDEVDTRVVRLGPDGGMRELGRLASLAATCP
                     LTPAVRLMRVGTRALRTAVLLPTGHVPGSRRLPVLMDPYGGPHAQRVLSARAVFRDSQ
                     WLADQGFAVVVVDGRGTPGRGPAWERAVHHELAEVTLADQVDALAAVVKELPDDLDAS
                     RVGIRGWSYGGYLAALAVLRRPDVFHAAVAGAPVTDWRLYDTFYTERYLGHPDVTGEV
                     YDRNSLLADAPRLRRPLMIVHGLADDNVVAAHTLRLSSALLAAGRAHTVLPLSGVTHM
                     TPQEVVAENLLHLQVSFLREALAQPATP"
     gene            6277..7161
                     /gene="mshB"
                     /locus_tag="VLC50_03125"
     CDS             6277..7161
                     /gene="mshB"
                     /locus_tag="VLC50_03125"
                     /EC_number="3.5.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014374795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010125 - mycothiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-1-D-myo-inositol-2-amino-2-deoxy-alpha-
                     D-glucopyranoside deacetylase"
                     /protein_id="HSF26488.1"
                     /translation="MTALPDRRLLLVHAHPDDETINSGVTMAHYVAAGAAVTLVTCTL
                     GEEGEILEPLYAQLAADQADQLGGYRIGELAAAMHELGVSDHRFLGGPGRYRDSGMMG
                     TPANEHARAFWQADVDEASRHLVEVVREMRPQVLLTYDENGGYGHPDHIQAHRVAMRA
                     VELAADPTVDVGGDPWEIPKVYWSTMPRSVLARGIELMREQGSDFFGVESVDDLPFVA
                     PDEVVTTAIRAPEWLEAKQAAMRAYPTQISVDGPFFALSNNVGLEAMGTEYYQLVRGK
                     RGPGSGTHGWEDDLFAGL"
     gene            7303..7605
                     /locus_tag="VLC50_03130"
     CDS             7303..7605
                     /locus_tag="VLC50_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005268708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF26489.1"
                     /translation="MTGFVQIIEWKTHRIDEVRALNEEWSSRFPRQQGPTRVVVAADR
                     DEPDSYVTMAEFASYEEAMKNSNDPVTSEFAARMAELCDGPPTFHNLDITRLEDRT"
     gene            7755..9731
                     /locus_tag="VLC50_03135"
     CDS             7755..9731
                     /locus_tag="VLC50_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HSF26490.1"
                     /translation="MGGRAGALMEGPGGGRAVIEAGAADVGSTRERRRLHRLAVLAVL
                     LGPPAAYLWWRILTGDAVDVLGLPGAVLGSIDWLVMAPILFFVALIALLGGSYLVSGR
                     SPHTLFRPEQIDVRLDDVVGIDVVKEEVVRSLNLFLAHKTFTAATGGRPRRGLLFEGP
                     PGTGKTHTAKAVAAEAGVPFLFASATSFQSSFYGATARKLRSYFTELRRLAAREGGAI
                     GFIDEFDAIAGTRHGMEFSTRPAGEPGWLPCSTMRALPASYAGMAGSRPPAVAGAGLV
                     SHRFGTADIAGPVVNELLVQLQSFDQPTGTQKLVGKLIDAINGFLPAHRALPKPAVEP
                     ANIMLIASTNRADGLDPALLRPGRFDRRLAFEPPDRAGRRALLDHFLAGKAHEAALDA
                     DEPRDTLAGLTAGYSPAMIEHLLDEALVNALRRGQTTMSWADLEAARLTEEVGLGQPV
                     GYTAHEKRLIATHEAGHAALAWLVAPHRRLDILSIIKRKSALGMLAHGDREDVFTQSR
                     TEMLALIQVSLAGQCAEETFFGDISTGPSGDLLYATNVAAQMVGACGMTGTLVSYAAV
                     QNSAFNDSNIVGRVLADERGRETVEALLQEQKTHVRRLVSEHRHLIEALRDALLERHE
                     LIGTQITDVIEAAARAHAEAGGVIDLRGADTPLA"
     gene            9736..10146
                     /locus_tag="VLC50_03140"
     CDS             9736..10146
                     /locus_tag="VLC50_03140"
                     /inference="COORDINATES: protein motif:HMM:NF040328.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6113 family protein"
                     /protein_id="HSF26491.1"
                     /translation="MQHPAEQQRHTGDLVAAAAVYVLVVVVGFGVGLIGSFSQAATRT
                     LVGVDLPVGLVASLVVTLAVIVGAGLSTGSRVGAGLAFAGWLLATMVMAVKRPEGDLV
                     VAATPLGLVWLFGGTVLGGLALAWPYGQRLRPRR"
     gene            10279..10737
                     /locus_tag="VLC50_03145"
     CDS             10279..10737
                     /locus_tag="VLC50_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012851502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HSF26492.1"
                     /translation="MAGRFATGVTVVTTCLDGRDHAMTANAFTSVSLDPLLVLVCVEK
                     EARFHDAIVESVLWTVNILDEHGRAASSWFATRGRPLIGQLDRFPHSRSTVTGAAVLD
                     VAMAVVDARTTAIHDGGDHSIVVGEVLTASARTGESAPLIYYRSRYRGLA"
     gene            10821..12653
                     /locus_tag="VLC50_03150"
     CDS             10821..12653
                     /locus_tag="VLC50_03150"
                     /inference="COORDINATES: protein motif:HMM:NF016202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HSF26493.1"
                     /translation="MIIDDNTDDVLPADPDGADRRAAWFAAGGLLALLLLLWAAAWVA
                     VGSGVPRGTTVLGVAIGGLDHDRAAATLSAELGPRSTAPIAVTVPGAGPTDAPTKSQV
                     DPAAAGLRLDVEATLEAAGGRSLNPVALVSALFGGHELDPVVTVDEPALKGAVAKLAT
                     TLGQPVVDGDVTFEEARAVPVQPASGREIDEAATEDRLRAAYLHESGPIELPSTVVAP
                     KVAPDEVTRAFEEFGRPAVSGPITISSGNATVSLAPERFAPALSMAPDEQGRLVPHVD
                     GAVLAKDAADELAGLERPARDAAVQIVSGKPQVIEGQTGLTIDPDVLGEGVLPALTSS
                     ERVATVSLTEVEPELTTAAVNALGIVEQVSSFTTRFPHASYRNTNIGRAAALIDGTLL
                     RPGETFSLNGIVGERTRANGFTEGFIIRNGRFETDLGGGVSQVATTTFNAAFFAGLKD
                     VEHHPHSLYISRYPEGREATVAWGSKDLRFTNDSPYGVLIDTAFSRSAPGRQGVITVS
                     IYSTKVYDVTAHKSARYRARSFPTVYDTSATCHAEGGVSGFDVDVTRVFHKDGKQVRR
                     ETMHTRYQPTPRVVCGPKPAPTPSPGTTVPAAGSMPAPSTSPAA"
BASE COUNT         1758 a         4667 c         4441 g         1824 t
ORIGIN      
        1 cgcgcctctc tcaccagcac tgctggtttt cgcccgatcg tggcgatacg ttcccgttgc
       61 ccgtcaaccg tcagccggag cacacccggg tctgggtggc cccccagcgg tcccccgcga
      121 ggagtagtcc ttgggtgctt acatcgctcg ccggatcctt cagatgatcc cggtcgtgct
      181 gggggccacg ttcctcatct actcgatggt ctggtacctg ccgagcgacc ctttcgcggg
      241 tcgctgtggc gaccgcccgt gccccgaggc ctacgtcgcc ctgatgcggg agaagttcaa
      301 cctcgacgac ccgctgttcg tctcctactt caagtacttc ggtgacctgc tcaccggcga
      361 cttcggggag acgttccagg gcctgcaggt cagggacgag atcctgcgcg cctacccggt
      421 gaccatgaag ctggctctga tcgccatcct catcgagatc ttcatcggga tcggggccgg
      481 tgtgctcgcc ggtctccgtc ggggcaagtt catcgacaac ctcgtgctgg tctcgacgct
      541 ggtggtcatc tcgatcccgg tcttcgtgat cggcaccacg ctgcagttct ggctcggcat
      601 caagtttccg atcttcccgc cgaccgtgtc ctccgaggcg acctggtact ccttgctgct
      661 gccaggcttc gtgctcgcca gcctctcgtt ggcctacgtc gcgaggctgg tgcgcacctc
      721 gatcggcgag aacatccgcg ccgactacgt gcgcaccgcg gtcgccaagg gcctgcccgg
      781 ccgacgggtg gtcgggctgc acgtcatgcg caactcgttg atcccggtga tcacgttcat
      841 cggcgccgac ttcggggctc tcctgggcgg ggcgatcgtc accgagggga tcttcaacat
      901 cgccggggtc ggcggtttgg ccttccgcgc catccggacc caggaggcga tgacggtcac
      961 cggcgtggtg acggtgctga tcctggtgtt cctgatcgtc aacctgctcg tcgacctgct
     1021 ctatggagtg ctcgacccga ggatccgtca tgactagcac tgtcaacgcc ccgtcaacga
     1081 gcccgaccga ggccgagccg gcgctgggca aaccgcgctc gctcggctcg gaggcgtggc
     1141 tggtcctgcg cggccgagcg atgttctgga tcgccctcgt gctcatcgcc gtcatcgtgc
     1201 tgatggcggc cttcccgggg ctgttcacgt cggtcgaccc ctacgccggt gacctctcgc
     1261 gctcgcggca ggcccccagc ctcgactcgc aggcctcctg gttcggctac gacagcgtcg
     1321 gccagtcggt ctacgcccgg gtcgtctacg gcgcccgggc atcgatcctg gtcgggatca
     1381 tcacgaccgt ggtggtgacg ctggtcggcg gcttcctcgg caccgtggcg gccttctacg
     1441 gccgccggac cgacaccctg atctcgcggg tcggcgacat cttcttcggc atcccgctgc
     1501 tgctcggtgc cctggtcgtg ctggtctcgt tcccgtccga cgagaacact cccagctggg
     1561 cgacgatcag caaggtcgtc ttcgccctgg cggtgctcgg ctggcccggc atcatgcgga
     1621 tcatgcgctc gagcgtgctg caggtgaaga gcggcgacta cgtcatggcc gcgcgagccc
     1681 tcggcgccag cgggtcgcgg atcatccgca agcacgtcgt gcccaacgcg gtggcgccag
     1741 tcatcgtcta cgccaccatc tcgttgggcg tctacatcac gatcgaggcg accttgagct
     1801 tcctcggcat cgggctgcag ccgccggtgg tctcctgggg cgtcatgatc agcgacgcgc
     1861 agaactacct gcgggtcgca ccgcacatgc tgttgttccc cagcgcgttc ctgtcggtca
     1921 ccgtcctcgc gttcatcatg ctgggcgacg tcgtccgcga cgccctcgac cccaagctgc
     1981 actaggaggc tgcgtccgtg gcgacccaag ccgtgaccct gcccagcggg gcgagcctgc
     2041 ccgacacctc acccctgctc gaggtgcgcg acctgcgggt cgagttccgc acccgtgagg
     2101 gggtggccaa ggcggtcaac ggcgtttcct acaccgtcga cgagggcgag accctggcgg
     2161 tgctcgggga gtctggatcg ggcaagagcg tcagcgccca ggccatcatg ggcatcatcg
     2221 acagcccccc cgggttcgtc accggcgggc aggtgctctt ccggggcgac gacctgctga
     2281 ccatgcccga ggacgagcgc cgcgcctacc gcggccgcaa gatcgcgatg atcttccagg
     2341 acgcgctgtc ctcgctcaac ccggtgttcc cggtgggctg gcagattgcc gagatgttcc
     2401 gggtccacga gcacctgagc cgcaaggagg cgcgggctcg ggcgatcgag ctcatggagc
     2461 gggtccggat cccggccgcc gccgagcggg tcgacgacta cccgcaccac ttctccggcg
     2521 gcatgcgtca acgcatcatg atcgctatgg cgttggcgct caaccccgac ctgctcatcg
     2581 ccgacgagcc gaccacggcc ctcgacgtca ccgtgcaggc gcagatcatg gagctgctcg
     2641 ccgacctgca gcgtgagagc aacatgggcc tgatcctcat cacccatgac ctcggcgtgg
     2701 tcgccgacgt ggccgacaag atcgctgtga tgtacgccgg gcgcatcgtc gagcaggcgc
     2761 cggtctatga catctacgcc aacccggccc acccctacac cgagggcctg ctgcagtcga
     2821 tcccccgggt cgggcagaag ggccagaagc tctccgcgat cgaggggctg ccgcccaacc
     2881 tcacccgcat cccgcccggt tgcgcctacc acccccgctg cccctacgcg cgcgacatct
     2941 gccgcaccga aaacccggcg ctgcgcacgg tcggccccga ccgcgtcagc gcctgccact
     3001 tcgccgagga ggtccttgat ggcggccgct agcctctcgc gcgaagtcgt cctcgacgcc
     3061 caagacctgg tcaagcactt cccgctgacc accggcgtcg tgatcaagcg ccgggtcggg
     3121 gcggtgcagg ccgtcgacgg tgtctcgttc cagctgcaca agggcgagac gcttggcgtc
     3181 gtcggcgagt cggggtgcgg caagtccacc ctcgccaagc tgctcatgcg gctcgaggag
     3241 ccgacctccg gtcacgccta cttcggcggc aaggacatct actcgctgcg cggacgggcg
     3301 ctgcgcgaca tgcgccggcg cgtgcagatc gtgtttcagg atccctactc ctcgctcaac
     3361 ccacggatga ccgtcggcga catcgtgggg gagccgttcg agatccaccc cgaggtggcg
     3421 cccaagggcg agcggcgcaa gcgggtccaa gagctgctcg acgtggtcgg gctcaacccc
     3481 gagcacatca accgctaccc gcaccagttc tcgggcggcc agcgccagcg catcggcatc
     3541 gcccgcggcc tcgcgctgcg gcccgacgtc atcatctgcg acgagccggt ctcggccctc
     3601 gacgtgtcgg tgcaggccca ggtcatcaac ctgctcgagg acctgcagag cgagttcggg
     3661 ctctcctaca tcttcatcgc ccacgacctg tcggtggtgc ggcacatctc cgaccgggtc
     3721 gccgtgatgt acctcggcaa gatcgtcgag atcggcgacg aggaccagat ctacagccgg
     3781 cccgcccacc cctacacgca ggcgctgctg tcggccgtgc cggtgcccga ccccacgctg
     3841 cggggccggc gcgagcagat cgtgctcgag ggcgacgtgc ccagcccggc caacccgccg
     3901 tcgggctgcc gcttccgcac ccggtgctgg aaggcgcagg agatctgcgc gtcggaggag
     3961 ccggccctgg tgcgccgccc gggggtcgac cacgagagcg cctgccactt cgccgaggtg
     4021 cgccagatcg tgcccgccag cgactgactg ggccgactcg tccccaggcc gtcagggggt
     4081 ggccggctgg gccagcgcct cccgcaggaa gctcacctgc aggtgcagca ggttctcggc
     4141 gacgacctcc tgcggggtca tgtgggtgac gcccgacagc ggcagcacgg tgtgcgcgcg
     4201 accggcggcc agcagcgccg aggacaggcg gagcgtgtgc gcggcgacca cgttgtcgtc
     4261 ggcgagcccg tgcacgatca tgagcgggcg gcgcagccgg ggggcgtcgg ccagcaggct
     4321 gttgcggtcg tagacctcgc cggtgacgtc ggggtggccg aggtagcgct cggtgtagaa
     4381 ggtgtcgtag agccgccagt cggtcaccgg tgcccccgcg accgcggcgt ggaagacgtc
     4441 gggccggcgc agcaccgcga gcgcggcgag gtagccgccg taggaccagc cgcggatccc
     4501 gacccgggag gcgtcgagat cgtcgggcag ctccttgacc acggcggcca gcgcgtcgac
     4561 ctggtcggcc agtgtcacct cggccagctc gtggtggacc gcacgctccc aggccgggcc
     4621 gcggcccggc gtgccgcggc catcgaccac gacgacggcg aagccctggt cggccagcca
     4681 ctgcgagtcg cggaagacgg cccgggccga gagcacccgc tgcgcgtgcg ggccgccgta
     4741 ggggtccatg agcaccggca gtcgccggga gccgggcaca tgaccggtcg gcagcagcac
     4801 ggcggtgcgc agggcccggg tgccgacccg catcagccgt acggcgggag tcagcgggca
     4861 ggtcgccgcc agcgaggcca gccggccgag ctcgcgcatg ccgccgtcgg ggccgagccg
     4921 cacgacccgg gtgtcgacct cgtcgtgctc gagagtgcgg ctggagacga tcgcgacgtc
     4981 gtcgcgcacc agaccggtgt gcacgccggg gctctccgcc agcccggtca cctccccgtc
     5041 ccagccgatc ctggccagca cgatctcggt cggctcggtc gaggcggtga cgagcgcgcc
     5101 ggtgccgtcg acgcctgcca cggcgcgcac ctgcaccccg accggcgaga acgcgacacc
     5161 gtcggcggcc agtcgccggc agccgtcggc gtccaccgtg gtcagcaggg cacccttagg
     5221 cgtccagcac ggcacgcccg cgaccaggtc gagccaggtg tcgtcggacg cctcgtgcac
     5281 ctggtagctg gtggcgctcg ccgcatcgac ggccagcacg acggcgcggc gctggtcgcg
     5341 gctcatcacc tgcaccagcg gatcgccgta cgacgtccag gacacccggg tgaggtaggg
     5401 cagcgccgtc cggtcccacg acacctcacg ccgtgcgccg tcgaggtcga ccagccacag
     5461 cgtcacctcg gcgtcgtcgc caccggcggc cgggtagcgc accgaggccg gagggagcgc
     5521 ggggtgggcc gggtcggcga tgtgccacac ggtgaccggc gactcgtcgg tgcgctgcac
     5581 cagcagcgaa cgcccatccg gcgcccacca gtagccccgg tcgcggtcca tctcctcggc
     5641 ggccacgaac tcggccaggc cccaggtgat ctgctccccg tcgggcgcgg cgagcacccg
     5701 ctcgggaccg ccggtgaacg gcaccacccg cagcgcgccg tcggccacga atgccaccca
     5761 ctcgccggtg gggtccggcc gcgggtcgat caccgggccg gtgacgtcga ggtcgcggca
     5821 ctggccggtc aacaggtcgg tgacgaacaa ccggccggac agcgcgaacg ccgcccgggt
     5881 gcccgcctcg tcggtggcgt aggagatgat gcccgcgccg ccctcccggg cccgctcacg
     5941 ccgggcgcgc tcggccggcg acaaccgctc ctgaccgccg tccaacaact cgtgcgggtc
     6001 ggccacgacg cgctcgagca gcccctcctc gacgatgtcg gccacccaca ggcacgcggc
     6061 ccggtcggtg ccgccgcgtg agcgcaggaa ggcgacccga ctccggtccg acgagatggt
     6121 gaactcgcgg ggcacgccga gggtgaagct cagggtgcgg gccttgaggc ggggatagct
     6181 catcgggagg tcggtctcgg tcaccggccc atcctggcag ggtggctcgg gtgcgcgtgc
     6241 ccacagccga tgctctacct gcgcgaggat ggggccatga cagcgctgcc tgaccgccga
     6301 ctcctgctcg tgcacgccca ccccgacgac gagacgatca acagcggggt cacgatggcg
     6361 cactacgtcg ccgcgggcgc cgcggtcacg ttggtgacgt gcacgctggg ggaggagggg
     6421 gagatcctcg agccgctcta cgcccagctc gccgccgacc aggccgacca gctggggggc
     6481 taccggatcg gcgagctggc ggcggcgatg cacgagctcg gggtcagcga ccaccggttc
     6541 ctcggcggcc cgggacgcta ccgcgacagc gggatgatgg gcaccccggc caacgagcac
     6601 gcccgtgcgt tctggcaggc cgacgtcgac gaggcgtcac gccacctcgt cgaagtggtc
     6661 cgcgagatgc gcccgcaggt cctgctcacc tatgacgaga acggcggcta cggccacccc
     6721 gaccacatcc aggcccaccg ggtcgccatg cgtgccgtcg agctggccgc cgacccgacc
     6781 gtcgacgtcg gcggcgaccc ctgggagatc cccaaggtct actggtcgac catgccccgc
     6841 tcggtgctgg cgcgcggcat cgagctcatg cgcgaacagg gcagcgactt cttcggcgtc
     6901 gagtcggtcg acgacctgcc gttcgtggcg cccgacgagg tggtcacgac ggcgatccgc
     6961 gcgccggagt ggctggaggc caagcaggcg gcgatgcggg cctaccccac gcagatcagc
     7021 gtcgacgggc cgttcttcgc gctgagcaac aacgtcgggc tcgaggcgat gggcacggag
     7081 tactaccagc tggtccgcgg caagcgtggc cccggcagcg gcacccatgg ctgggaggac
     7141 gacctcttcg ccggcctctg acctctgccc tccctgcact gatcacgcgc gcctggcggg
     7201 ctggcgagcg cgcccggggg cctcgtggcg gcttgacgcc gtgcccgggc ggggcgacgt
     7261 tgtgagcggg ctgccgacca cccgacgggc cagggaggcc agatgaccgg tttcgtccag
     7321 atcatcgaat ggaagaccca ccggatcgat gaggtgcgcg ccctcaacga ggagtggagc
     7381 tcgcgcttcc cgcggcagca ggggccgacc cgcgtggtcg tcgccgccga ccgcgacgag
     7441 cccgactcct acgtgacgat ggcggagttc gcctcctacg aagaggcgat gaagaactcc
     7501 aacgacccgg tgacctcgga gttcgccgcc cggatggccg agctgtgcga cggcccgccg
     7561 acgttccaca acctcgacat cacccggctc gaagatcgca cctgagcccc ggccccggca
     7621 gatacgccgt acgagtgaaa cggagcgccc gctctgtgat cactgtgggt gacgggtgga
     7681 cactgcggtc ggactcgggc cgggagagtc gtcacggaga gtgacggttc cggtgtcggg
     7741 ggagggatgg ggccgtgggt gggcgagccg gcgcgctgat ggagggcccg ggcgggggcc
     7801 gggcggtcat cgaggcgggc gccgccgacg tcgggtcgac ccgcgaacgc cgtcgcctgc
     7861 accggctggc ggtcctcgcg gtgctgctcg ggccgccggc ggcctacctc tggtggcgca
     7921 tcctcaccgg tgacgccgtt gacgtgctcg ggctgcctgg cgccgtgctg ggcagcatcg
     7981 actggctcgt catggcgccg atcctcttct tcgtcgccct gatcgcgctg ctcggtggct
     8041 cctacctggt gtccggccgc tcaccgcaca cgctgttccg gcccgagcag atcgacgtgc
     8101 gcctcgacga cgtggtcggc atcgacgtgg tcaaggaaga ggtcgtccgc tcgctcaatc
     8161 tgttcctggc ccacaagacg ttcaccgccg ccaccggggg ccggccccgg cgcggcctgc
     8221 tcttcgaggg cccgcccggc accggcaaga cgcacaccgc caaggcggtg gccgccgagg
     8281 ccggcgtgcc gttcctgttc gcctcggcca cgtcgttcca gtcctcgttc tacggtgcca
     8341 ccgcgcgcaa gctgcgcagc tacttcaccg agctgcgtcg gctggccgcc cgcgagggtg
     8401 gggcgatcgg gttcatcgac gagtttgacg cgatcgccgg cacccgccac ggcatggagt
     8461 tctccacccg cccggcgggc gagccgggct ggctgccgtg ctcgacgatg cgagccctgc
     8521 cggcgtcgta cgccgggatg gccggctcga gaccgccggc ggtggccggg gccggcctgg
     8581 tcagccaccg gttcggcacc gcagacatcg ccgggccggt cgtcaacgag ctgctcgtgc
     8641 agctgcagtc cttcgaccag cccaccggca cgcagaagct cgtgggcaag ctcatcgatg
     8701 cgatcaacgg gttcctgccg gcgcaccggg cgctgcccaa gccggcggtc gagccggcca
     8761 acatcatgct catcgcctcg accaaccgcg ccgacggcct cgacccggca ctgctgcggc
     8821 ccgggcgctt cgaccggcgg ctcgcgttcg agcccccgga ccgggccggc cgccgggccc
     8881 tgctcgacca cttcctcgcc ggcaaggcgc acgaggcggc cctcgacgcc gacgagccgc
     8941 gcgacacgct ggccgggctg accgccggct acagcccggc catgatcgag cacctgctcg
     9001 acgaggcgct ggtcaacgcg ctgcggcgcg ggcagaccac gatgtcgtgg gccgacctcg
     9061 aggccgcccg gctcaccgaa gaggtgggcc tcggccagcc ggtcggctac accgcgcacg
     9121 agaagcggct catcgccacc cacgaggccg gccacgccgc cctcgcctgg ctggtcgccc
     9181 cgcaccggcg cctggacatc ctctcgatca tcaagcgcaa gagcgcgctg ggcatgctgg
     9241 cccacggcga ccgggaggac gtgttcaccc agtcgcgcac cgagatgctc gcgctgatcc
     9301 aggtgtcgct ggccggccag tgcgccgagg agaccttctt cggcgacatc tcgaccggcc
     9361 ccagcggcga cctgctctat gccaccaacg tggccgccca gatggtgggc gcctgcggga
     9421 tgaccggcac gctggtctcc tacgcggcgg tgcagaactc cgcgttcaac gacagcaaca
     9481 tcgtcgggcg ggtgctggcc gacgagcgcg gccgcgagac cgtcgaggcg ctgctgcagg
     9541 agcagaagac gcacgtacga cggctggtca gcgagcaccg tcatctgatc gaggcgctgc
     9601 gcgacgccct gctcgagcgc cacgagctca tcggcaccca gatcaccgac gtgatcgagg
     9661 cggcggcccg cgcgcacgcc gaggccggcg gggtcatcga tctgcgcggg gccgacacgc
     9721 cgctagcctg aggcggtgca gcacccggcc gagcagcagc gccacaccgg tgacctggtg
     9781 gccgccgcgg cggtctacgt gctggtcgtg gtcgtgggct tcggcgtcgg tctgatcggc
     9841 agcttctccc aggccgcgac ccggaccctc gtgggcgtcg acctgccggt gggactggtg
     9901 gccagcctcg tcgtgaccct ggccgtcatc gtcggcgcgg gcctgagcac cgggtcgcga
     9961 gtcggggccg gcctggcctt cgccggctgg ctgctggcga ccatggtcat ggcggtcaag
    10021 cgaccggagg gcgacctcgt cgtggcggcc acccccttgg gtctggtctg gctgttcggc
    10081 ggcacggtgc tgggcgggct cgccctggcc tggccctacg gccagcggct gcgtccgcgc
    10141 cgctgaggcg gccccacccg cggcggggcc cacgggcgcg gcgagtaggg tcggtgcgtg
    10201 acggacactc gtcccgaccc cacacccgac ctcgccgctg cccttgccgg cggggtcgat
    10261 gccggcacct tccgcgcggt ggccgggcgg tttgcgaccg gggtcacggt cgtcaccacc
    10321 tgtctcgacg ggcgcgacca cgccatgacc gccaacgcct tcacgtcggt ctcgctcgac
    10381 ccgctgctcg tgctggtgtg cgtggagaag gaggcccgct tccacgacgc gatcgtggag
    10441 tcggtgctgt ggaccgtcaa catcctcgac gagcacggcc gggcggcctc ctcatggttc
    10501 gccacccgcg gccgaccctt gatcggccag ctcgaccggt tcccgcactc gcgcagcacc
    10561 gtcaccggcg cggcggtgct cgacgtggcg atggccgtgg tcgacgcgcg caccaccgcg
    10621 atccacgacg gcggcgacca ctcgatcgtg gtcggagagg tgctcaccgc gtcagcccgc
    10681 accggtgagt cggcgccgct gatctactac cgcagccgct accgcggcct cgcctgaccc
    10741 ggtctgaccg agccgaccct taccatggtg gctcccgcgc gccgcgaccg cggcggcgac
    10801 cccagggggt cccccacaac gtgatcatcg acgacaacac tgacgacgtg ctgcctgccg
    10861 atcccgacgg tgccgaccgc cgggccgcgt ggtttgcggc cggcggcctg ctggccctcc
    10921 tgctcctgct ctgggccgcg gcctgggtcg cagtcgggtc gggcgtgccc cggggcacga
    10981 ccgtgctcgg cgtggccatc ggcggcctcg accacgaccg ggcggccgcc accctgagcg
    11041 ccgagctcgg tccgcggtcg accgcgccga tcgcggtgac ggtgcccggc gctgggccca
    11101 ccgacgcacc gacgaagtcg caggtcgacc cggcggcggc cgggctgcgg ctggacgtcg
    11161 aggcgacgct cgaggccgcc ggcggtcgca gcctcaaccc ggtggccctg gtctccgcgc
    11221 tcttcggcgg gcacgagctc gacccggtcg tcacggtcga cgagccggcc ctgaagggcg
    11281 ccgtggccaa gctcgccacc acgctcgggc agcccgtggt cgacggcgac gtgacgttcg
    11341 aggaggcccg ggcggtgccg gtgcagccgg cgtccgggcg cgagatcgac gaggccgcga
    11401 cggaggatcg gctcagggcg gcctacctgc atgagagcgg ccccatcgag ctgccctcga
    11461 ccgtcgtcgc acccaaggtc gcccccgacg aggtgacccg cgcgttcgag gagttcgggc
    11521 gtccggccgt gtccggtccg atcacgatct cctccggcaa cgcgacggtg tcgctcgcgc
    11581 ccgagaggtt tgccccggcg ctgtcgatgg cccccgacga gcagggtcgg ctggtgcctc
    11641 atgtcgacgg ggcggtgctg gccaaggacg ccgccgacga gcttgccggg ctcgagcgcc
    11701 cggcccgtga tgccgccgta cagatcgtgt ccggcaagcc ccaggtgatc gagggacaga
    11761 ccggtctgac catcgacccc gacgtgctcg gtgagggcgt gctgccggcg ctgaccagct
    11821 ccgagcgggt cgcaacggtc agcctgaccg aggtcgagcc cgagctgacc accgcggcgg
    11881 tcaatgcgct gggcatcgtc gagcaggtgt cgtcgttcac gacccgtttc ccgcacgcga
    11941 gctaccgcaa caccaacatc ggccgggccg ccgcgctcat cgacggcacc ctgctgcgcc
    12001 cgggcgagac gttcagcctc aacggcatcg tgggcgagcg gacccgggcc aacgggttca
    12061 ccgagggctt catcatccgc aacggccggt tcgagaccga cctgggcggc ggggtctcgc
    12121 aggtggcgac cacgacgttc aacgcggcgt tcttcgccgg gctcaaggac gtcgagcacc
    12181 acccgcacag cctctacatc agccgctacc cggagggccg cgaggccacg gtcgcctggg
    12241 ggtcgaagga cctgcggttc accaacgaca gcccctacgg cgtgctcatc gacacggcgt
    12301 tcagccgcag cgcccccggg cgccaaggcg tgatcaccgt gtcgatctac agcaccaagg
    12361 tgtacgacgt caccgcgcac aagtcggccc gctatcgggc gcggtcgttc cccaccgtct
    12421 acgacaccag cgccacctgc cacgccgagg gtggggtcag cggcttcgac gtcgacgtca
    12481 cccgggtctt ccacaaggac ggcaagcagg tgcggcgcga gacgatgcac actcgctacc
    12541 agccgacccc acgggtggtc tgcgggccca agccggcgcc gacgccgtcg cccggcacca
    12601 ccgtgccggc cgccgggtcc atgccggcgc cgtcgacctc gccggcggcc tagccgcggc
    12661 ggtccgggtc gtcaccggcc cggcgtgcgg
//
LOCUS       DATIBB010000087         4982 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_1096206, whole genome shotgun sequence.
ACCESSION   DATIBB010000087 DATIBB010000000
VERSION     DATIBB010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 4982)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4982)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4982
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1096206"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..258
                     /locus_tag="VLC50_03155"
     CDS             <1..258
                     /locus_tag="VLC50_03155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF26494.1"
                     /translation="VTAAADALAPDIGAAGAVAAGSAGSGLASEVFSTLLSTLGDVTD
                     MTIGLDGPDMVLRLRLPGGQRDAAAADQRGLERDGGQPVTT"
     gene            360..761
                     /locus_tag="VLC50_03160"
     CDS             360..761
                     /locus_tag="VLC50_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015801178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod C-terminal
                     domain-containing protein"
                     /protein_id="HSF26495.1"
                     /translation="MFDAIAIAGSGVTVNRKWLDAVSDNIANINTVRPTSGDAFRAKY
                     VVATAVDYGTGTGGTRVAGITLGNPEGRLAYDPTNPLADAEGNVRLPDIDLGSQMTQM
                     LIAQRAYQSNLAVVDRAHDAYQAALQLGRNA"
     gene            758..1099
                     /gene="fliE"
                     /locus_tag="VLC50_03165"
     CDS             758..1099
                     /gene="fliE"
                     /locus_tag="VLC50_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013882503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HSF26496.1"
                     /translation="MTIGPIGAIGMPLPSLPVAPTGTTSPLAGARAAGAASTTSGAEF
                     ASALAQGLEGLQATQSHADGLAVQAATGDLTDVHDYMIAATEASLATDLTVAVRNKAV
                     EAFTEIMRMPV"
     gene            1099..2712
                     /gene="fliF"
                     /locus_tag="VLC50_03170"
     CDS             1099..2712
                     /gene="fliF"
                     /locus_tag="VLC50_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015625762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HSF26497.1"
                     /translation="MKRTLTGGLTRAQRLFAGFTSGQKTITAIAVLALVIGGALFSQW
                     VTKPSYAPLFSHLSTTDASAIVDKLGGAGVPFQLTDGGTTILVPQDKVYATRLQMSGE
                     GLPVGDSGGYALLDKQGLTTSEFQQQVGFQRALEGELKKTVESLDGVRSAAVHLAIPQ
                     KDVFTSDKDSPTASVLVSMGSGDRLATTQVQAIVNLVASSVTGMTPEDVTVADSTGRV
                     LSAAGQPAGLAAGDQRAQQTSDFEARTANALQDMVDQVVGSGHAVVKVTADLDYDQTS
                     TTTERYVSDPENPPLADTTTKESYQGSGASVGGVLGPDNAGVPMPGAGTDTKGTTYTK
                     DSVTRNNAVGKVVEVRKAAPGAIRKLNVGILLDTRTAGTVDPAELQTLVSSAIGIDPA
                     RGDTIEVTRLPFDETAAEAAEKALAAERDAAGKESMVSLAKTGGLVLLVLAVLVLAFL
                     SSRKRRVELSPEERVQLEVLQRELAARGETPALDEVDELPAIEPARAPVDPHLAQVAA
                     ARDEIGELLEAQPAEVAQLLRGWLADRRS"
     gene            2715..3737
                     /gene="fliG"
                     /locus_tag="VLC50_03175"
     CDS             2715..3737
                     /gene="fliG"
                     /locus_tag="VLC50_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009433 - bacterial-type flagellum basal
                     body, C ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HSF26498.1"
                     /translation="MSTKTAELSGVRKAAVLLVQMGRERAAKILAEMRENEVEELTAE
                     IVRLRDVDPDVADEVVEEFHHLVSARRYAGQGGLAFAQELLEASVGGDRAQELIARLT
                     AAMSEVPFHFLHRADPRQILSFVQDEHPQTIALVLAHLRAEQSSMILSGLAPELQADI
                     AHRIAVMDRTSPEIIRQVESMLERKLSSVLTPSDLAAVGGLEPLVQIINRADRATERL
                     ILEGLEGRDPELAEEIRSRMFMFEDIVTLDDRAVQLVLRQVESADLATALKGVREDVR
                     DKVMRNLSERAVENLVEEIDMLGPVRLRTVEEAQVKVVQVIRSLEETGQIAVRRGGDD
                     DDAFVE"
     gene            3754..4491
                     /locus_tag="VLC50_03180"
     CDS             3754..4491
                     /locus_tag="VLC50_03180"
                     /inference="COORDINATES: protein motif:HMM:NF014196.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliH/SctL family protein"
                     /protein_id="HSF26499.1"
                     /translation="MVTTLPPVVSVVLRGPAAAEARTARLDADLRHSPWAQRHSADPR
                     LVDPQLEQAFADAAAEVREAARAQGYAAGWAEGCRLGLEAGRAETVAAAAREAEATAR
                     RQTEFAQALQGLSRAAAALEATVAPAAATIDRLAIDAAVELVELLLDRELALAQTPAL
                     DAVRRALALTPAGRPVVVRLHPDDHAVLSALGAPTLDREITLVADPSVERSGAIAECD
                     ATRVDAQLGPALARVRAVLSGDAGAAA"
     gene            4488..>4982
                     /locus_tag="VLC50_03185"
     CDS             4488..>4982
                     /locus_tag="VLC50_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014374699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF26500.1"
                     /translation="MSLDALLDRARTAARPRVTGQVRGVLGLTVTVGGVDAAVGDLVE
                     VATPHGVLATEVVAVDPDGLSCTPLGELTGVRVGAEAHAVGGALTVPVGEALRGRVLD
                     GLGRPIDGGSSLSALPRVPVEQRVQTHAAAPASPQSTARTRASAGPSWASTRVASHSA
                     SAPDR"
BASE COUNT          711 a         1835 c         1823 g          613 t
ORIGIN      
        1 gtcacggccg ccgcggacgc gctggccccc gacatcggag ccgcgggcgc ggtggccgcg
       61 ggcagcgccg gctcggggtt ggcgagcgag gtgttcagca ccctgctctc gaccctgggt
      121 gacgtcaccg acatgaccat cgggctcgac ggcccagaca tggtgcttcg gctgcgcctg
      181 ccgggcgggc agcgcgatgc cgcagcggca gaccagcgcg gcctcgagcg cgacggcggg
      241 cagcccgtca ccacctgaat gtcctcaggt cgggtgcacg caggccgaag gggagcctga
      301 gccaggacgg ctcgccccgc gccacggacc ggcgacatcg actctgtcga ggagcgcccg
      361 tgttcgatgc catcgccatc gccggcagcg gggtcaccgt caaccgcaag tggctcgacg
      421 cggtcagcga caacatcgcc aacatcaaca cggtccgccc gaccagcggc gacgcgttcc
      481 gggccaagta cgtcgtcgcg accgcggtcg actacggaac cggcaccggc ggcacccggg
      541 tcgccgggat cacgctgggc aacccggagg gccggctcgc ctacgacccg accaacccgc
      601 tggccgacgc cgagggcaac gtgcgcctgc ccgacatcga cctgggcagc cagatgacgc
      661 agatgctcat cgcccagcgg gcctaccagt ccaacctcgc ggtcgtcgac cgcgcccacg
      721 acgcctacca ggcggcgctg cagctcggga ggaatgcgtg accatcggcc ccatcggagc
      781 catcgggatg ccgttgccgt cgctgccggt cgcgcccacg ggtacgacga gcccgctggc
      841 cggcgcccgc gcggccggcg cggcgagcac caccagcggc gccgagttcg ccagcgcgct
      901 ggcccagggg ctggagggtc tgcaggccac ccagagccac gccgacgggc tcgccgtaca
      961 agcggccacc ggtgacctca ccgacgtgca cgactacatg atcgccgcga ccgaggcgtc
     1021 actggccaca gacctgaccg tcgccgtacg caacaaggcc gtggaggcgt tcaccgagat
     1081 catgcggatg ccggtctgat gaagcgcacc ctcaccggcg gcctgacccg agcccagcgg
     1141 ctgttcgccg ggttcacctc cgggcagaag accatcaccg cgatcgcggt gctcgcgctg
     1201 gtcatcggcg gcgccttgtt cagccagtgg gtgaccaagc ccagctatgc cccgctgttc
     1261 agccacctgt cgaccaccga cgcgtccgcg atcgtcgaca agctcggcgg cgcgggggtg
     1321 cccttccagc tcaccgacgg cggcaccacg atcctcgtgc cgcaggacaa ggtctatgcc
     1381 acccggctgc agatgagcgg cgagggcctg ccggtcggtg acagcggcgg ctacgcgctg
     1441 ctcgacaagc agggcctgac cacctcggag ttccagcagc aggtcggttt ccagcgcgcg
     1501 ctggagggcg agctcaagaa gaccgtcgag tccctcgacg gggtgcggtc cgcggccgtg
     1561 cacctggcca tcccgcagaa ggacgtgttc acctccgaca aggacagccc gaccgcctcg
     1621 gtgctcgtgt cgatgggctc cggcgaccgg ctggccacca cccaggtgca ggccatcgtc
     1681 aacctggttg cctccagcgt caccgggatg acccccgagg acgtcaccgt cgccgactcg
     1741 accgggcggg tgctctcggc cgccgggcag cccgccgggc tggcggccgg tgaccagcgg
     1801 gcccagcaga cctcggactt cgaggcccgt acggcgaacg cgttgcagga catggtcgac
     1861 caggtcgtcg gctccggcca cgccgtcgtc aaggtgaccg ccgacctcga ctacgaccag
     1921 acctcgacca cgaccgagcg ctacgtcagc gacccggaga acccgccgct ggccgacacg
     1981 acgaccaagg agtcctacca gggctcgggt gcctcggtcg gcggcgtgct cggcccggac
     2041 aacgccggcg tgccgatgcc cggcgccggc accgacacga aaggcacgac ctacacgaag
     2101 gactcggtca cccgcaacaa cgcggtcggc aaggtcgtcg aggtgcggaa ggccgcgccg
     2161 ggcgcgatcc gcaagctcaa cgtgggcatc ctgctcgaca cccgcaccgc cggcaccgtc
     2221 gacccggccg agctgcagac cctggtgtcc tcggcgatcg gcatcgaccc ggcccgcggc
     2281 gacaccatcg aggtgacccg gctgccgttc gacgagaccg ccgccgaggc cgccgagaag
     2341 gcgctggccg ccgagcggga cgcagccggc aaggagtcga tggtctcgct ggccaagacc
     2401 ggcggactcg tgctgctcgt gctcgccgta ctcgtgctgg ccttcctctc ctcgcgcaag
     2461 cgtcgggtcg agctcagccc cgaggagcgg gtgcagctgg aggtgctcca gcgtgagctg
     2521 gccgcccgcg gcgagacgcc ggcgctcgac gaggtcgacg agctgccggc gatcgagccg
     2581 gcccgcgccc cggtggaccc gcacctcgcc caggtggccg cggcccgcga cgagatcggt
     2641 gagctgctcg aggcgcagcc ggccgaggtg gcccagctgc tgcgcggctg gctcgccgac
     2701 cggaggtcgt gacgatgtcc accaagaccg ccgagctctc cggcgtgcgc aaggccgctg
     2761 tactgctggt ccagatgggc cgggagcgcg ccgccaagat cctcgccgag atgcgggaga
     2821 acgaggtcga ggagctcacc gccgagatcg tgcgactgcg cgacgtcgac ccggacgtgg
     2881 ccgacgaggt cgtcgaggag ttccaccacc tggtgtccgc gcgccgctac gccgggcagg
     2941 gcgggctggc gttcgcccag gagctgctgg aggcctcggt cggcggcgac cgggcccagg
     3001 agctgatcgc ccggctgacc gcggcgatga gcgaggtgcc gttccacttc ctgcaccggg
     3061 ccgacccgcg ccagatcctt tccttcgtgc aggatgagca cccgcagacg atcgcgctgg
     3121 tgctggccca cctgcgcgcc gagcagtcct cgatgatcct gtccggactg gcccccgagc
     3181 tgcaggccga cattgcgcac cggatcgcgg tgatggaccg cacctcgccg gagatcatcc
     3241 gtcaggtcga gtcgatgctc gaacgcaagc tgtccagcgt gctgaccccc agcgacctgg
     3301 ccgcggtggg cgggctcgag ccgctggtgc agatcatcaa ccgggccgac cgggccaccg
     3361 agcggctcat cctcgagggc ctggaggggc gcgaccccga gctggccgag gagatccgct
     3421 cgcggatgtt catgttcgag gacatcgtca cgctcgacga ccgcgcggtg cagctggtgc
     3481 tgcgccaggt ggagagcgcc gacctggcca ccgcgctcaa gggagtgcgc gaggacgtgc
     3541 gcgacaaggt catgcgcaac ctgtcggagc gggccgtgga aaacctcgtc gaggagatcg
     3601 acatgctcgg cccggtgcgg ctgcgcacgg tcgaggaggc ccaggtcaag gtcgtgcagg
     3661 tgatccgttc gctcgaggag accggccaga tcgccgtacg gcgtgggggt gatgacgatg
     3721 acgcgttcgt cgagtgaaac gaccgcgccg cgcgtggtga caaccctgcc gccggtcgtc
     3781 tccgtggtgc tgcgcgggcc ggccgccgcc gaggcgcgca ccgcccggct ggacgccgac
     3841 ctgcggcaca gcccgtgggc gcagcgccat tccgcagatc cgcggctggt tgacccgcag
     3901 ctcgagcagg cgttcgccga cgcggccgcc gaggtgcgcg aggcggcccg ggcgcagggc
     3961 tatgccgccg gctgggccga ggggtgccgg ctcgggctgg aggccggccg ggccgagacg
     4021 gtcgctgccg cggcccggga ggccgaggca acagcgcgtc gtcagaccga gtttgcccag
     4081 gcgctgcaag ggttgtcacg cgcggccgct gcgctggagg ccaccgtcgc cccggccgcg
     4141 gcgaccatcg accggcttgc catcgacgcg gcggttgagc tggtggagct gctgctcgac
     4201 cgcgagctgg ccctcgcgca gacccccgcg ctcgacgcgg tccgccgggc gctggccctc
     4261 acgccggccg gacgcccggt cgtcgtacgg ctgcatcccg acgaccacgc ggtgctgagc
     4321 gcgctcggcg cgccgacgct cgaccgcgag atcaccctgg tcgccgaccc gtccgtcgaa
     4381 cgatccgggg cgatcgccga gtgcgacgcc acccgggtcg acgcccagct cggcccggcg
     4441 ctggcccggg ttcgcgcggt gctctccggc gacgccgggg ccgcggcatg agtctggacg
     4501 cgctgctcga ccgggcccgc accgcggccc ggccgcgggt gaccggccag gtccgcgggg
     4561 tgctcgggct gaccgtgacc gtcggcggcg tggacgccgc ggtcggcgac ctggtcgagg
     4621 tggccacccc gcacggggtg ctggccaccg aggtggtcgc cgtcgatccc gacgggctgt
     4681 cgtgcacgcc gctgggcgag ctgaccgggg tgcgggtcgg cgccgaggcg cacgcggtcg
     4741 gcggggcgct gaccgtgccg gtcggcgagg cgctgcgcgg gcgggtgctc gacgggctgg
     4801 gccggccgat cgacggcggc tcctctctgt ccgcactgcc ccgggtgccg gtcgagcagc
     4861 gcgtccagac tcatgccgca gccccggcat cgccgcagag caccgcgcgc acccgggcca
     4921 gcgccgggcc gagctgggcg tcgacccggg tggcgtcgca ctcggcgagc gccccggacc
     4981 gt
//
LOCUS       DATIBB010000088         8041 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_835632, whole genome shotgun sequence.
ACCESSION   DATIBB010000088 DATIBB010000000
VERSION     DATIBB010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 8041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8041
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_835632"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(158..1045)
                     /locus_tag="VLC50_03190"
     CDS             complement(158..1045)
                     /locus_tag="VLC50_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018844065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HSF26501.1"
                     /translation="MRVAVTGASGFIGGHLVPALRTAGHDVVRLVRRAPSAPDEVSWD
                     PQSGVVDTAALGRVDAAIHLAGAGIGDRRWTTGYKRTVIDSRVEGTSTLARALAAMDP
                     RPAVLVSASGIGYYGETGPTPTDESGPLDDTFAAQVCQQWESAADPAREAGIRVVHPR
                     SAPVIARRGGAYGRLRLVLRTGLGGPLGSGRQYWSWITLEDEIRAMLFALEQARLEGP
                     VNFVAPNPVTNAELMRACARALHRPALVPVPAFALRLVLGEFATELLISQRIVPRKLL
                     DAGFSFTHAEIDEAAKTLA"
     gene            complement(1084..3030)
                     /gene="sucB"
                     /locus_tag="VLC50_03195"
     CDS             complement(1084..3030)
                     /gene="sucB"
                     /locus_tag="VLC50_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020553024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase, E2 component,
                     dihydrolipoamide succinyltransferase"
                     /protein_id="HSF26502.1"
                     /translation="MSFSITMPALGESVTEGTVTRWLKQVGERVEVDEPLLEVSTDKV
                     DTEIPSPAAGVVTQIIVGEDETVDVGTELAIIGEAAGSDGEVVDAPSSAQPAAPAPAP
                     EQPAPAAQSAAAEPAAAPAQPAAPAPAAAPAPAAAPAPASTPPASTGGTAVTMPALGE
                     SVTEGTVTRWLKQVGDTVATDEPLLEVSTDKVDTEIPSPAAGVVSQILVGEDETVEVG
                     TTLALIGGTANGAAPSAAAATPAPAPAEAAAPSPALAAPAAAPVAAPAPAPAAPTGNA
                     LADEAAMHAQAEEQAEERAVNGASATPSRAATPAPAAPAAAPAPAAAPAPAAEAGDSS
                     TYVTPLVRKLAAEHGVDLASINGTGVGGRIRKQDVLAAAEAAKAAKAAAAAAASAASP
                     ATVTASAPSGSPTQPVSPLRGTTEKMSRLRKVIATRMVESLQVSAQLTTVVEVDVTRI
                     ARLRQRAKADFEAREGVKLSFLPFFALATVEALKAHPKLNASIEGDQIVYHGSENLGV
                     AVDTERGLLVPVIKDAGDLNLAGIARKVADLAERTRTNNVGPDELGGGTFTLTNTGSR
                     GALFDTPVINQPQVAILGTGAVVKRPVVVDDPGLGETIAIRSMVYLALTYDHRLVDGA
                     DAARFLVDIKTRLEEGRFEAEVGL"
     gene            complement(3065..4456)
                     /gene="lpdA"
                     /locus_tag="VLC50_03200"
     CDS             complement(3065..4456)
                     /gene="lpdA"
                     /locus_tag="VLC50_03200"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019136922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HSF26503.1"
                     /translation="MADQAGSTDPFDVVILGGGSGGYACALRAAELGLTVALVEKDKL
                     GGTCLHRGCIPTKALLHAGEVADSARESAAFGVLATFDSIDMGGVNKYKDGVVARLHK
                     GLQGLIKSKNITYVEGTGRLVAPNTVEVNGTRLTGRNVVLATGSYSRSLPGLEIGGLV
                     MTSEQALALDFLPSSVIVLGGGVIGVEFASVWRSFGAEVTIVEALPRLAPAEDEACSK
                     ALERAFRKRKINFKTGVRFSGVTQDINGVHVSLESGETLDADLLLVAVGRGPSTAGMG
                     YEEAGVSIERGFVVTDERLRTSVAGVYAVGDIVRGLQLAHRGFAQGIFVAEEIAGLAP
                     APIDELGIPRVTYCEPEIASIGLTEAAASEKYGAERVEAYEYNLGGNGKSQILGTTGF
                     VKVVREKDGPVVGVHMVGARVGELVSEAQLIYNWEAFPEEVAALIHAHPTQGEALGEA
                     HLALAGKPLHAHA"
     gene            4608..4937
                     /locus_tag="VLC50_03205"
     CDS             4608..4937
                     /locus_tag="VLC50_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012920313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF26504.1"
                     /translation="MGLFRRRARKGVQRRADAGDVAHLVEFAGAHRGVEAFVEPPTAV
                     TSTTVVFVADSGEWTRRRVNDARAAHELANRLGIPSYDAAVVGYPKRMRDWNARRGAE
                     RNDGLTG"
     gene            complement(4954..5628)
                     /locus_tag="VLC50_03210"
     CDS             complement(4954..5628)
                     /locus_tag="VLC50_03210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF26505.1"
                     /translation="MTVTAPALTRAAGVAAVAAGVMFIGVQIGHPDLDATSITTTDVY
                     LRDSLKVLMAVLALVGITGMYLSQIRRNGVLGLIGYAVLAAAYLLIMCGVFAAAYVLP
                     EIARANPGYVNDVIAVDTGRGSVEGDIGGLQTVIQLRGAAYLAGGLLFGIALYRAHVL
                     ARWASALLAVGGVVSVVLSLMPDAFYRLLALPNGIAMIGLGYSLWRTTRTSTTAPAPS
                     VTAATE"
     gene            5772..6527
                     /locus_tag="VLC50_03215"
     CDS             5772..6527
                     /locus_tag="VLC50_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012950208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4386 domain-containing protein"
                     /protein_id="HSF26506.1"
                     /translation="MTTSAGAAAALRAPSQAEQRTARIMGAWFLGTFVFSIPAFWFYD
                     PLLDHASYVVGSGEDTRVAIGALLEILLAVSGIATAVVIFPIARRVNESVALGYVAAR
                     TVESILILVGVLSLMSIVALRQDLVDAGGASEALVDVSRSLLAVRDQTALLGPQFCAG
                     LGNGILLGYLMWRSMLLPRQLVVFGLVGGPLAVLGGVGVLLGAWDNPSAPLAALTVLE
                     IVWELSLSVWLLVRGFRPSPVLTGPPVTAAPAS"
     gene            complement(6595..7338)
                     /locus_tag="VLC50_03220"
     CDS             complement(6595..7338)
                     /locus_tag="VLC50_03220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF26507.1"
                     /translation="MSRKLVQTVASLTAKQLRVTVAMLAALALGVGGAAAAVGSLNAA
                     TVANDTVAEETIPTESPEPVPTAEATDGASDGSTLVLAGSESLEGEADTGLGTPRNQD
                     PEACTIPAEFLPATEETPEVPAEGEVTGEVEENGEVEENGEVEELATHVWRNHGEFVR
                     SVATNKELVDHAGAVQWAAQSDCGKPLVSVNGEDAEADVETGTEEELKAESLTTEKSA
                     KTEHDQRWHAKKHNNKAAHGQEHAATNGR"
     gene            complement(7453..>8041)
                     /locus_tag="VLC50_03225"
     CDS             complement(7453..>8041)
                     /locus_tag="VLC50_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020130027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HSF26508.1"
                     /translation="GLPVNVTGWAACAENMPSGSAQRPSDVITIYGGRTVEVLNTDAE
                     GRLVLADALVAASGEQPDVIVDVATLTGAQMVALGGRTSAIMSNDDELRAQVHDAAQR
                     AGEAMWPMPLPEELRATLDSPVADLANMGDRFGGMLVAGLFLKEFVGEGLRWAHLDIA
                     GPAYNSGEPHDYTPRGGTGVAVRTMLTLAEDLAAS"
BASE COUNT         1051 a         2819 c         2937 g         1234 t
ORIGIN      
        1 cagcccgtcc agcccggcgg ccagccccgg gccggggtag accccggcca ccgccgacgc
       61 gacctgcgcg tcgccggggc tgccccacgg ctcaaacggc tcgaacacgt gtacgacact
      121 agccggaccc accgacagac actgccgcgc cgacgggtca cgccagggtc ttggccgcct
      181 cgtcgatctc ggcgtgggtg aagctgaagc cggcgtcgag cagcttccgc ggcacgattc
      241 gctgactgat cagcagctcg gtcgcgaact cgccgaggac cagtcggagc gcgaacgcgg
      301 gaacgggcac gagagcgggc cggtgcagcg cccgagcgca tgcccgcatg agctcggcgt
      361 tggtcaccgg gttgggcgcc acgaagttga ccgggccctc cagccgggcc tgctcgagcg
      421 cgaacagcat ggcccggatc tcgtcctcga gggtgatcca gctccaatac tgccgtccag
      481 agccgagtgg cccgccgagt ccggtgcgca gcacgagccg gagtcgcccg taggcgccgc
      541 cgcgacgagc gatcacgggg gcgctacggg gatggaccac ccggatgccg gcctcgcgcg
      601 ctgggtcggc ggcgctctcc cactgctggc acacctgtgc ggcgaacgtg tcatcgagcg
      661 gtcccgactc gtcggtcggg gtgggcccgg tctcgccgta gtagccgatc ccggatgccg
      721 acacgagcac cgccgggcgc gggtccatcg cggcgagcgc gcgggcgagc gtcgacgtac
      781 cctcgacccg gctgtcgatc accgtgcgct tgtagccagt ggtccaccgg cgatcgccga
      841 tcccggcgcc ggccaggtgg atcgccgcat cgacccgccc cagcgcggcg gtgtccacga
      901 ccccggactg cgggtcccag ctcacctcgt caggtgcgga gggggcacgg cggaccagtc
      961 gtacgacgtc gtggccggcc gtgcgcaggg cagggaccag gtgcccgccg atgaaacccg
     1021 aggcacccgt cactgccacc cgcatgccga cccccctctt cgtcgtacgg cgtgggcgcg
     1081 ggtctagagg ccgacctccg cctcgaaccg gccctcttcg aggcgggtct tgatgtccac
     1141 caggaagcgc gccgcgtcgg cgccgtcgac gagccggtgg tcgtaggtca gggcgaggta
     1201 gaccatcgac cggatcgcga tcgtctcgcc gaggcccggg tcgtcgacca cgacgggccg
     1261 cttcaccacg gcgccggtgc cgaggatggc gacctggggc tggttgatga ccggggtgtc
     1321 gaacagcgcc ccgcggctgc cggtgttggt cagcgtgaac gtgccgccgc ccagctcgtc
     1381 ggggccgacg ttgttggtcc gggtgcgctc ggcgagatcg gcgaccttgc gggcgatccc
     1441 ggcgaggttg aggtcgccgg cgtccttgat cactggcacc agcaggccgc gctcggtgtc
     1501 gacggccacg ccgaggttct cgctgccgtg gtagacgatc tggtcgccct cgatgctcgc
     1561 gttgagcttg gggtgggcct tgagcgcctc gaccgtggcc agcgcgaaga acggcaggaa
     1621 cgagagcttg acgccctcgc gtgcctcgaa gtcggccttg gcgcgctggc gcagccgggc
     1681 gatgcgggtg acgtcgacct cgacgaccgt ggtgagctgc gccgagacct gcagagactc
     1741 gaccatgcgg gtggcgatga ccttgcgcag ccgcgacatc ttctcggtgg tcccgcgcag
     1801 cgggctgacg ggctgggtcg gggatccgct cggtgcgctc gcggtgaccg tggccgggga
     1861 ggcggcggag gcagccgcgg cggcggcggc cttggcggcc ttggcggcct cggcggcggc
     1921 gagcacgtcc tgcttgcgga tccgcccgcc gacgccggtg ccgttgatcg aggccaggtc
     1981 gaccccgtgc tcagcggcca gcttgcgcac gagcggagtg acgtaggtcg aggagtctcc
     2041 ggcctcggcc gccggcgccg gagcagccgc gggcgccgga gcagccgcag gagccgcggg
     2101 cgcgggggtg gcggcccggc tcggggtcgc gctggcgccg ttgacggcgc gctcttccgc
     2161 ctgctcctcg gcctgggcgt gcatcgccgc ctcgtcggcc agcgcgttgc cggtcggcgc
     2221 tgccggggcg ggcgcggggg cagccacagg cgcggcggcg ggtgccgcaa gggcgggcga
     2281 cggagcggct gcctcggcgg gggcgggcgc gggagtggcc gcggcggcgg agggcgccgc
     2341 gccgttggca gtgccgccga tcagcgcgag cgtggtgccg acctcgacgg tctcgtcctc
     2401 gccaaccaag atctggctga ccacgccggc ggcgggggag gggatctcgg tgtcgacctt
     2461 gtcggtggac acctcgagca gcggctcgtc agtggcgacg gtgtcgccga cctgcttgag
     2521 ccagcgcgtc acggtgccct cagtcacgct ctcaccaaga gccggcatcg tgacggcggt
     2581 gccgcccgtg ctggccgggg gcgtgcttgc cggtgccggc gccgctgcgg gtgccggcgc
     2641 cgctgcgggt gccggggcgg ctggctgggc gggagccgcc gcgggctcgg cggcggccga
     2701 ctgtgcggcc ggggcgggct gctcgggagc cggtgccggc gccgcgggct gcgcgctgga
     2761 gggcgcgtcg acgacctcgc cgtcggatcc cgcggcctca ccgatgatcg ccagctcggt
     2821 gcccacgtcg accgtctcgt cctcgccgac gatgatctgc gtgacgacgc cggcggcggg
     2881 ggaggggatc tcggtgtcca ccttgtcggt ggacacctcg agcaggggct cgtcgacctc
     2941 gacgcgctcg cccacctgct tcaaccagcg cgtgacggtt ccctccgtga cgctctcgcc
     3001 gagggcgggc atcgtgatcg agaacgacat gggcactccg atctctcgtg cagttgtgcg
     3061 gacgtcaggc gtgagcgtgc agcggcttgc ccgccagcgc gaggtgtgcc tcgccgagcg
     3121 cctcaccctg cgtggggtgg gcgtggatca acgcggcgac ctcctcgggg aacgcctccc
     3181 agttgtagat cagctgggcc tcgctcacca gctcgccgac ccgggcgccc accatgtgga
     3241 caccgaccac cgggccgtcc ttctcgcgga cgaccttgac gaagcctgtg gtgccaagga
     3301 tctggctctt gccgttgccg ccgaggttgt actcgtaggc ctccacccgc tccgcgccgt
     3361 acttctcgct ggcggccgcc tcagtcaggc cgatcgaggc gatctccggc tcgcagtagg
     3421 tcacccgcgg gatgccgagc tcgtcgatcg gcgcgggcgc gagcccggcg atctcctcgg
     3481 ccacgaagat gccctgggcg aagccgcggt gcgcgagctg caggccgcgg acgatgtcgc
     3541 cgaccgcgta gaccccggcc acggaggtgc gcagccgctc gtcggtgacc acgaacccac
     3601 gctcgatgct cacaccggct tcctcgtagc ccatgccggc ggtgctcggg ccacgcccga
     3661 cggcgaccag caggaggtcg gcgtcgaggg tctcgcccga ctcgaggctg acgtggacgc
     3721 cgttgatgtc ctgggtcacc ccgctgaacc gcacgccggt cttgaagttg atcttgcgct
     3781 tgcggaacgc gcgctcgagc gccttggagc aggcctcgtc ctctgccgga gccagccggg
     3841 gcagcgcctc gacgatggtc acctcggcac cgaaggagcg ccagacgctc gcgaactcga
     3901 caccgatgac cccaccgccg agcacgatca ccgacgacgg caggaagtcg agcgccagtg
     3961 cctgctcgct cgtcatcacg agcccgccga tctcgaggcc aggcaggctg cgggagtaag
     4021 agccggtcgc gagtacgacg ttgcgcccgg tcaggcgcgt gccgttgacc tcgaccgtgt
     4081 tgggggcaac gagccggccg gtgccctcga cgtacgtgat gttcttggac ttgatcagtc
     4141 cctgcaggcc cttgtgcagc cgggcgacga caccgtcctt gtacttgttg accccgccca
     4201 tgtcgatcga gtcgaacgtc gcgagcacac cgaacgccgc gctctcgcgg gcggagtcgg
     4261 ccacctcgcc ggcgtgcagg agggccttgg tcgggatgca gccgcggtgc aggcaggtcc
     4321 cgccgagctt gtccttctcc acgagggcca ccgtgagccc cagctcggca gcgcgcagcg
     4381 cgcaggcgta gccgccgctg cctccgccga ggatcacgac gtcaaagggg tcggtgctgc
     4441 cagcctggtc ggccacggaa cactccttgt gcgaggctgc ggtcgctgcg tgcccggggt
     4501 ggggcgtcgc gatccacatc ttgtcactgc ggccacggct ggcagcaatg cgggccaccc
     4561 tgacggcgct cctctaccgt ggagggccac aggtgagagg ggtacccgtg gggcttttcc
     4621 gtcgtcgggc ccgcaagggg gtccagcgtc gcgctgacgc gggcgatgtc gcgcacctcg
     4681 tcgagttcgc cggcgcgcac cggggtgtcg aggcgttcgt cgagccgccg accgcggtga
     4741 cgagcacgac cgtggtcttc gtcgccgact cgggtgagtg gacgcgccga cgggtcaacg
     4801 acgcccgagc ggcccacgag ctggccaacc ggctgggcat cccgtcctac gacgcggcgg
     4861 tggtcggcta ccccaagcgg atgcgtgact ggaacgcgcg ccggggcgcc gagcgcaacg
     4921 acgggctgac cggctgagcc gctcctgctg tcctcactcc gtggcggccg tgaccgacgg
     4981 cgccggcgcg gtggtgctcg tccgcgtggt gcgccacagc gagtagccga ggccgatcat
     5041 ggcgatgccg ttgggcagag ccagcagccg atagaaggca tccggcatca gggacagtac
     5101 gacgctgacc acgccgccga ccgccaggag cgcggacgcc cagcgggcca gcacgtgcgc
     5161 ccggtagagc gcgatgccaa acagcaggcc acccgccagg taggccgcgc cgcgcagctg
     5221 gatcacggtc tgcagccccc cgatgtcgcc ctcgacgctg ccgcgcccgg tgtcgaccgc
     5281 gatgacgtcg ttgacgtaac ccgggttggc ccgggcgatc tcaggcagca cgtaggcggc
     5341 agcgaagacg ccgcacatga tgagcaggta ggccgcggcc agcaccgcgt agccgatgag
     5401 cccgaggacg ccgttgcggc ggatctggct cagatacatg cccgtgatgc cgacgagggc
     5461 aagcacggcc atgagcacct tcagggagtc tcggaggtag acgtcggtcg tcgtgatcga
     5521 ggtcgcgtcc aggtctggat gaccgatctg gaccccgatg aacatgacgc cggcggcaac
     5581 cgcggcgacg cctgcggctc gggtgagggc gggggcggtg acggtcatct ccgactcctt
     5641 gctccgaggt cggcgcggcg ggcgtcgtga cctgtggtgg ccccttcacg gtgacacctg
     5701 gacccaacag tcatgacgag aactgagcgg aatgcagcgc cgcactgcgg tgctgctctt
     5761 acgctccaga agtgacgact tcggccggag ccgctgcggc tctgcgcgct cctagccagg
     5821 cggagcaacg aaccgcgcgc atcatgggag cctggttcct gggcacgttc gtcttctcga
     5881 tccccgcctt ctggttctac gaccccctct tggaccacgc cagctacgtc gtcggcagcg
     5941 gcgaggacac ccgggtggcg attggcgcgc tgctggagat tttgttggcg gtctccggca
     6001 tagccaccgc cgtggtcatc tttcccatcg cgaggcgggt caacgagagc gtcgccctcg
     6061 gctacgtcgc cgcgcgcacc gtggagtcga tcctcatcct ggtcggcgtc ctgagcctga
     6121 tgtcgatcgt ggccctgcgc caggatctcg tcgatgccgg aggcgctagt gaggcgctcg
     6181 tcgacgtgag ccgctcgctg ctcgccgtac gcgaccagac cgctctgctc ggaccccagt
     6241 tctgcgccgg actggggaac gggatcctgc tcggctacct gatgtggcga tccatgctcc
     6301 ttccgcggca gctggtggtg ttcggcctcg tgggcggtcc cttggccgtg ctcggcggag
     6361 taggcgtgct gctcggggcg tgggacaacc cctccgcacc gctggccgca ctcaccgtgc
     6421 tggagatcgt ctgggagctc tcgctgagcg tgtggctgct cgttcgaggc ttccggccgt
     6481 cacctgtcct caccggcccg ccagtgaccg cagccccggc cagctagcgc agaagcgcgc
     6541 ccccggatgc tccggggacg cgcttctgag cccgtcgtgg gcgagaggcg gtggtcagcg
     6601 accgttggtc gcggcgtgct cctgcccgtg tgccgccttg ttgttgtgct tcttggcgtg
     6661 ccaacgctgg tcgtgctcgg tcttcgcgga cttctcggtg gtgagcgact cggccttgag
     6721 ctcttcctca gtcccggtct cgacgtccgc ctcggcgtcc tcgccgttga cgctcaccag
     6781 cggcttgccg cagtcggact gtgcagccca ctggaccgct cccgcgtggt cgacgagctc
     6841 cttgttggtc gcgaccgacc tgacgaactc gccgtggttg cgccagacgt gggtggcgag
     6901 ctcttctacc tcgccgttct cctcgacctc gccgttctcc tcgacctcgc cagtcacttc
     6961 gccctcggcg gggacctcag gggtctcctc ggtggcaggg aggaactccg ctgggatcgt
     7021 gcaggcctct gggtcctggt tccggggggt cccgaggccg gtgtcagctt cgccctcaag
     7081 gctttccgag cccgccagca ccagggtcga gccgtcgctt gccccatcgg tggcctcggc
     7141 cgtcggcacc ggctcggggc tctccgtcgg gatggtctcc tcagcgacgg tgtcgttggc
     7201 gaccgtggcc gcattgaggg agccgaccgc tgcggcggcg ccaccgacgc cgagggcgag
     7261 ggcagcgagc attgcgacgg tcacgcgcaa ctgcttggcg gtcagcgacg ccacggtctg
     7321 gacgagcttg cgggacacgg gtcctcctgc gggctagcgg gcgattcacc cgtacatcgc
     7381 aggccaggaa cgtaccgtta cagactattc ggacaattcc gcaagagtcc cccgatcggc
     7441 gcactctggt gctcaggacg cggccaggtc ctcggcgagc gtcagcatgg tgcgtacggc
     7501 gaccccggtg ccgcccctgg gcgtgtagtc gtgcggttcg ccgctgttgt aggcggggcc
     7561 ggcgatgtcg aggtgggccc agcgcaggcc ctcgccgacg aactccttga ggaacagccc
     7621 cgctacgagc attccgccaa accggtcacc catgttggcc aggtcggcga cgggggagtc
     7681 cagcgtcgcg cgcagctcct cgggcagcgg catcggccac atcgcctcgc cggcccgctg
     7741 ggcggcgtcg tgcacctggg cgcgcagctc gtcgtcgttg gacatgatcg ccgaggtacg
     7801 tccgcccagg gccaccatct gagcgccggt cagcgtcgcc acgtcgacga tcacgtcggg
     7861 ctgctcgccg ctcgccgcga ccagggcgtc ggcaagcacc agccggccct cggcgtcggt
     7921 gttgagcacc tccacggtgc gtccgccgta gatcgtgatg acgtcggagg ggcgctgggc
     7981 cgagccggag ggcatgttct ctgcgcaggc cgcccacccg gtgacgttga ccggcaggcc
     8041 c
//
LOCUS       DATIBB010000089         5880 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_574455, whole genome shotgun sequence.
ACCESSION   DATIBB010000089 DATIBB010000000
VERSION     DATIBB010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 5880)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5880)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5880
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_574455"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..862
                     /locus_tag="VLC50_03230"
     CDS             <1..862
                     /locus_tag="VLC50_03230"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020386584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HSF26509.1"
                     /translation="ATRVLGATYTPHCARIHPARLVRGLARAVERRGGVIHEQTAVRA
                     IRPRRVITSRGRISARYVVRATEGFTPALARHRRVLAPAYSLMVATEPLPASFWAGAG
                     LAAYETFADHRHLIIYGQRTADDRLAFGGRGAPYHWGSRVRPGYDRVPRVFAALARTL
                     RDLFPSLAEARLTHAWGGPLGIPRDWMASVGLDRARGLAWAGGYVGDGVSTSNLAGRT
                     LADLLLGRDTPLTALPWVGHVSPHWEPEPLRWLGINAGLRLAKAADLEERLTRRPARL
                     GVLLDRLTGG"
     gene            897..1211
                     /locus_tag="VLC50_03235"
     CDS             897..1211
                     /locus_tag="VLC50_03235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF26510.1"
                     /translation="MGTDRDAARTDVAARVEQVADLLHEAAETHHRVYRITDGADADW
                     ASWYADWLARLSELPEVLGREPVRAELTHELVAADHDYTATGATEPWEQFYAARLVRD
                     LG"
     gene            1198..1731
                     /locus_tag="VLC50_03240"
     CDS             1198..1731
                     /locus_tag="VLC50_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014741245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HSF26511.1"
                     /translation="MTSADPAGSPRHRLVLMRHAKAEAAASADTERALTGDGARDAAA
                     LGRWLAARGVVPDQVVASSARRAVETWEQTARGLDAQPEPVLDGRIYANTVDDLLAVI
                     HEVPESVGTLAVVGHNPTMSRLALLLDDALGDATARADLMIGLPTCAAAVFELDRDWA
                     DVQAGSATLIAFSAPRG"
     gene            1833..2627
                     /locus_tag="VLC50_03245"
     CDS             1833..2627
                     /locus_tag="VLC50_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020013373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HSF26512.1"
                     /translation="MDDRSRLLASYDEQLRTDAETPSAVRVSLLGPLRLVTFAGGRGF
                     VTYQDLGGADAEAIGRLVGAALAHYQADPAITRVEWKTRGHDHAPGLHQSLLGHGFAA
                     EEPESIMIGAAQRLAVDVPLPDGVRLRQVTEESDVRAMSAMSAQAFGDSVHEAAEMAN
                     ALLGRLSLDDGMELWVAEADGHIVSAGRLEPVPGTDFAGIWGGSTLKEWRGRGIYRAL
                     TAARARSALRMGKTLIHSDSTEYSRPILERSGFLKVSTTTPYLWRR"
     gene            2667..3089
                     /locus_tag="VLC50_03250"
     CDS             2667..3089
                     /locus_tag="VLC50_03250"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HSF26513.1"
                     /translation="MDRQTAQDWLDRYVDAWLSYDPDDIGALFADDVAYRYHPSGEPV
                     VGREAVVASWLGEGESADASTRDAPGTYEARYAPVAVDGDTVVATGTSRYRERPGGPV
                     VRTYDNCFVMRFDGEGRCREFTEYYVRRPDSPAVASPE"
     gene            complement(3134..3571)
                     /locus_tag="VLC50_03255"
     CDS             complement(3134..3571)
                     /locus_tag="VLC50_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007449417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HSF26514.1"
                     /translation="MDAKDAEILSVLRKDARTPYVTLGARVGLSANAVADRVRRMQRA
                     GVIRRFTVDVDESAAEPGRLVVFIDVTLRPDTKAEQFERAVRRLPDVVEAMHVTGGGD
                     YLLRAHVADVARLDRLLRVLKTECGAQSTSTRVALRSVIPEAR"
     gene            3642..4274
                     /locus_tag="VLC50_03260"
     CDS             3642..4274
                     /locus_tag="VLC50_03260"
                     /inference="COORDINATES: protein motif:HMM:NF013933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HSF26515.1"
                     /translation="MSVTSALLAGIVAGLGVAVPLGAIGVLLLQTGLVAGWRVGAAGA
                     LGVALVDLAYAGLVVTAGAAVAEPIARHSTVIRLVGSAVLFVVAAIGLRSVLRPGSAS
                     SATNVGLAAPPGTAPGAVLARFVGLTALNPLTAVYFAALVAGFSGRFDAIGRIAFVAG
                     VFAASLAWQLVLITVGATTGHRASPRMRGALGLAGALLVAAYAVRLAVGE"
     gene            4428..4892
                     /locus_tag="VLC50_03265"
     CDS             4428..4892
                     /locus_tag="VLC50_03265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF26516.1"
                     /translation="MQTTMEYIEAVAPEDVLATLRWLTAHGYEVSRAVGAGEHMGSAL
                     VVFSHSREVTVLRDRGQWDISVALSPGGTPLSLAVLNAARLGVAWDPGPHRPLDEPLA
                     PQLPEGMIWHETLPLVLEWLDQPGATAAVEAAAAQARESTTRWWKEMRQDRA"
     gene            complement(5070..>5880)
                     /locus_tag="VLC50_03270"
     CDS             complement(5070..>5880)
                     /locus_tag="VLC50_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005448879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HSF26517.1"
                     /translation="LRTLFLAGERLDPETYRWASDTLGIPVIDNWWQTETGWPIVANL
                     RGLEPMPVKAGSPTVAVPGFDVQILDERGNQVGANVEGSICIKLPMPPGTLPTLWQDD
                     ERYIASYLSAFPGYYLTGDGGYIDEDGYVFVMGRTDDVINVAGHRLSTGAMEAVLAQH
                     PAVAECAVIGVADSLKGQVPRGFVVLKAGVDVDHEVLAGELVAAVRDQVGPVAAFREV
                     SVVMALPKTRSGKILRKTMRQIADGQDAPVPSTIEDPAVLDRLRPTLLRQP"
BASE COUNT          823 a         2042 c         2101 g          914 t
ORIGIN      
        1 ggccacccgg gtgctcggcg cgacctatac gccgcactgc gcccgcatcc acccggcccg
       61 gctggtgcgt gggctggccc gggcggtcga gcggcgcggc ggggtgatcc acgagcagac
      121 cgcggtgagg gcgatccggc cgcggcgggt catcacgtcg cggggacgga tcagcgcgcg
      181 ctacgtcgta cgggccaccg aggggttcac cccggcgctg gcccggcacc ggcgcgtgct
      241 ggcgccggcc tactcgctga tggtggccac cgagccgctg cccgcgtcgt tctgggccgg
      301 tgccggactg gcggcgtacg agacgtttgc cgaccaccgg cacctgatca tctatggcca
      361 gcgcaccgcc gacgaccggc tggccttcgg cggccgtggg gcgccctacc actggggctc
      421 gcgggtgcgg ccgggctacg accgggtgcc gcgggtgttc gccgcgctcg cgcggacctt
      481 gcgcgacctg ttcccgtcgc tggccgaggc ccggctgacc cacgcctggg gcggaccgct
      541 ggggatcccg cgcgactgga tggcctcggt cggactcgac cgggcccgcg ggctggcctg
      601 ggccggcggc tacgtcggcg acggggtctc gacgtccaac ctggccgggc gcacgctggc
      661 cgacctgctg ctcggtcgcg acactccgct gaccgcgctg ccctgggtgg gccatgtctc
      721 gccgcactgg gagcccgagc cgctgcgctg gctgggcatc aacgccggac tgcgactggc
      781 caaggccgcc gacctcgagg agcggctgac ccgtcggcca gcccggctgg gcgtgctgct
      841 ggaccggctt accggtgggt gatgcgcgct cgccgtcagc ccgccatgct gaacccgtgg
      901 gaaccgacag ggacgccgcc cggaccgacg tggccgcgcg ggtcgagcag gtcgccgacc
      961 tgctgcacga ggcggccgag acgcaccacc gggtctaccg gatcactgac ggggccgacg
     1021 ccgactgggc cagctggtac gccgactggc tggcccggct ctccgagctg cccgaggtgc
     1081 tcggccgcga accggtgcgc gccgagctga cccacgagct tgtcgccgcc gaccacgact
     1141 acaccgccac aggtgcgacc gagccgtggg agcagttcta cgccgcgagg ctggtccgtg
     1201 acctcggctg atccggcggg gtcaccgagg caccggctgg tgctgatgcg ccacgccaag
     1261 gctgaggcgg cggccagcgc cgacaccgaa cgggccctga ccggtgacgg cgcccgcgac
     1321 gcggccgccc tcggtcggtg gctcgccgcg cggggcgtcg tacccgacca ggttgtggcc
     1381 tcgtcggctc ggcgtgcggt cgagacctgg gagcagaccg cccgggggct cgatgcccag
     1441 ccagagccgg tgctcgacgg gcggatctac gccaacaccg tcgacgacct gctggcggtg
     1501 atccacgagg tgccggagtc ggtgggcacc ctggccgtgg tcgggcacaa cccgacgatg
     1561 agccggctgg ccctgctgct cgacgacgca ctgggcgacg ccaccgcccg cgccgacctg
     1621 atgatcggcc tgcccacctg tgccgccgca gtcttcgagc tggaccgaga ctgggccgac
     1681 gtgcaggccg ggtcggcgac gctgattgcc ttcagcgcac cccggggcta accacgcgcg
     1741 agcgtggcga agacatagcc ggcgacgtcc ggcgccgcgt gccatcggaa atcggctggc
     1801 agctcagaca tgcaatcggc aatcatgcgc gggtggatga tcgcagccgg ttgctcgcgt
     1861 cgtacgacga acagttgcgg accgatgcgg agacaccgag cgcagtccgg gtgagcctgc
     1921 ttggtccgtt gcggctggtg acgttcgcag gtggccgcgg cttcgtgacc taccaagacc
     1981 tcggcggcgc cgatgccgaa gcgattggcc ggctggtcgg ggccgcgctc gcgcactacc
     2041 aggcggatcc cgcgatcact cgggtggagt ggaagacgcg cgggcatgac cacgcgccag
     2101 gactgcacca aagcctgctt ggccacgggt tcgccgcgga ggagccggag tcgatcatga
     2161 tcggcgcggc gcagcgactc gcggtcgatg ttcccttgcc cgacggcgtc agactgcggc
     2221 aggtcaccga ggagagcgat gttcgcgcga tgagcgcgat gagcgcgcag gcgtttggag
     2281 actctgtcca cgaggccgcg gagatggcca atgccttgct gggtcggctc tccctcgatg
     2341 acggcatgga gctgtgggtc gccgaggccg acgggcacat cgtctccgct gggcgactcg
     2401 agccagtccc cgggaccgac tttgcgggca tctggggagg ctcgaccctc aaggagtggc
     2461 ggggtcgcgg aatctatcgc gctttgaccg cggcacgggc gcgctcggcg ctgcgcatgg
     2521 gcaagacgct catccacagc gactccaccg agtactcgcg accgatcctg gaaaggtccg
     2581 gctttctcaa ggtgtccacc acgaccccgt acctctggcg tcgctgacgc ggacgttgct
     2641 ggcccgaggc cggctaggtt gggccgatgg accgacagac cgctcaagac tggctcgatc
     2701 ggtacgtcga cgcgtggctg tcctatgacc ccgacgacat cggggcgctg ttcgccgacg
     2761 acgtggccta ccgctaccac ccctccggcg agccggtcgt cggccgggag gccgtcgttg
     2821 cgtcctggct cggcgagggc gagtcagctg atgcgtccac ccgcgacgca ccgggcacct
     2881 atgaggcccg ctatgccccg gtcgcggttg acggcgacac cgtggtggcc accggcacgt
     2941 cgcgctaccg cgagcggccc ggcgggccgg tggtgcgcac ctatgacaac tgcttcgtga
     3001 tgcgcttcga cggtgaagga cgctgccggg aattcaccga gtactacgtt cgccgccccg
     3061 actcgccagc cgtcgcttca cccgaatagc ccggccccgc cggagtcgtg gctggccgtg
     3121 catccgcatg gcttcaccgc gcctcgggga tgaccgagcg cagcgccacc cgggtcgagg
     3181 tgctctgcgc accgcactcg gtcttgagca cccgcagcag ccggtcaagc cgagccacgt
     3241 cagccacgtg cgcccgaagc aggtagtcgc ccccgccggt cacgtgcatc gcctcgacca
     3301 cgtccggcag gcgccgtacg gcgcgctcga actgctcggc cttggtgtcc gggcgcagcg
     3361 tcacgtcgat gaacaccacc agccggcccg gttcggcggc ggactcgtcg acatcgacgg
     3421 tgaatctgcg gatcacgccg gcccgctgca tgcgccggac ccggtcggcg accgcgttgg
     3481 cgctcagacc cacccgagct cccaaggtga cgtacggcgt ccgcgcatcc ttgcgcagca
     3541 cgctgaggat ctcggcgtcc ttggcatcca tggtgcggtt ctctctgtcg ttgagcggct
     3601 gacacgggat gatccgccgt gccgccccca tcatggccga cgtgtcagtg acctccgccc
     3661 tgctcgccgg gatcgtggcc gggctcggtg tggcggtgcc gctgggcgcg atcggcgtgc
     3721 tgcttctgca gaccgggctg gtcgcgggct ggcgggttgg tgccgcaggc gcgctggggg
     3781 tggcgctggt cgacctggcc tatgcgggcc tggtggtcac ggccggggcg gccgttgccg
     3841 agccgattgc caggcactcc accgtgatcc gactcgtcgg ttctgccgtc ctctttgttg
     3901 tcgcggccat cgggttgcgg tcggtgctgc gcccgggcag tgcttccagt gctaccaacg
     3961 tggggctcgc ggcacctcca gggaccgcac cgggcgccgt gctggcgcgc ttcgtcggtc
     4021 tgacagcgct caacccactc accgcggtct acttcgcggc attggttgct ggattctccg
     4081 gccgattcga cgccatcggc cgcattgcct ttgttgccgg ggtgttcgcg gcctcactcg
     4141 cctggcagct cgttctcatc accgtcggag cgactaccgg tcaccgcgct tcaccgcgga
     4201 tgagaggcgc tttagggctg gctggcgccc tcctcgtcgc cgcctacgcc gtccgcctcg
     4261 ccgtcggtga atgactcacg agccacgacg caccggccac cagggggcag accgtggtcg
     4321 gcaagttcgc taggtgctaa tacgtcccct tgctcaatgg gcggcttctc ggcaagacga
     4381 gcgaataccc ggcaataggc atcgcctgct ggggcagact tgcgcgggtg cagaccacga
     4441 tggagtacat agaagccgtc gcgccggaag atgtactcgc cacccttcgt tggttgactg
     4501 ctcatggcta cgaggtgtct agggccgtcg gcgcaggaga gcacatgggt agcgcccttg
     4561 tggtcttctc gcactcacgc gaagtcacgg tcctgcgaga tcgaggtcag tgggacatct
     4621 cagtcgcgct gtcgccgggc ggaacgccgc tctcgctagc cgttctcaac gcggcacgac
     4681 ttggcgttgc ctgggatccc ggaccgcatc ggccgctgga tgagccgctg gcgccgcagt
     4741 tgccagaggg catgatctgg cacgaaacgc tgccgctggt gttggagtgg cttgaccaac
     4801 caggtgctac ggcggccgtc gaggcggctg ccgcccaggc aagggagagc acgacgcggt
     4861 ggtggaagga aatgcggcaa gaccgcgcat aagtgccgct cgcgctcggt cgggagcgcg
     4921 ccgcacgcgc gctctcttga cttgaaatag caacacaact cgttgagcca tcgcggtgtt
     4981 ccgaccccac tgggaccttg ctctgcatcc tgcaggccgc gatatgcccg tctgcccgtc
     5041 gtgcaaaggt tgcttgatcg cgacgagggc taaggctggc gcaggagggt cgggcggagg
     5101 cggtcgagga cggcggggtc ctcgatggtc gacggcaccg gcgcgtcctg accgtcggcg
     5161 atctggcgca tggtcttgcg caggatcttg cccgagcgag tcttgggcag cgccatcacc
     5221 acggacacct cgcggaatgc cgccaccggc cccacctgat cgcgcaccgc ggccaccagc
     5281 tcgccggcga gcacctcgtg gtcgacgtcg acgccggctt tgagcacaac aaagcctcgc
     5341 ggcacctggc ccttgagcga gtcggctacg ccgatcaccg cgcactcggc gaccgcgggg
     5401 tgctgcgcga gcaccgcttc catcgcgccg gtcgagaggc ggtgcccagc gacattgatg
     5461 acgtcgtcgg tgcgacccat cacgaagaca tagccgtcct catcgatgta gccgccatcg
     5521 ccggtgaggt aatagccggg gaaggccgac agataggacg cgatgtagcg ctcgtcgtcc
     5581 tgccacagcg tcggcagggt gcccggcggc atcggcagct tgatgcagat cgagccctcg
     5641 acgttggcgc cgacctggtt gccgcgctcg tcaaggatct gtacgtcgaa gccgggcacc
     5701 gcgacggtcg gcgagccggc cttgaccggc atcggctcga ggccgcgcag gttggccacg
     5761 atcggccagc cggtctcggt ctgccaccag ttgtcgatca cgggaatgcc gagcgtgtcg
     5821 ctggcccagc ggtaggtctc ggggtcgagc cgctcccccg cgagaaagag cgtgcgcagg
//
LOCUS       DATIBB010000090         9246 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_574766, whole genome shotgun sequence.
ACCESSION   DATIBB010000090 DATIBB010000000
VERSION     DATIBB010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 9246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9246
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_574766"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1632)
                     /locus_tag="VLC50_03275"
     CDS             complement(<1..1632)
                     /locus_tag="VLC50_03275"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HSF26518.1"
                     /translation="MTPVASLRAAPLGVGRVLAELLPGRARRLRRAWVGHDRAHIEVR
                     GAHREGTEPFARRLEDALGRVEGVHWAEVNAVLGRVVVAFDGEQIDVDDLVDIVEGVE
                     EAHELHEEAFPAERPEHPGDAEPLHRQVASLAADALGLGLSSIGRALGRGPLTEEVAS
                     LLSLVDSTPRLREQAERRLGTATADLLLATANAVSQGIAQGPLGLLVDATHRLAQVGE
                     ITARRVVWHAREPELHGQPSDERTAPLSLAPRPVPMPAGPVERYGDRAAAAAMLGAAT
                     TWIATRNHNDAVAALVSGTPKAARLTREVFASTLGRLLWRRGIVPLDATALRRLDRVD
                     TVVVDAPVLMTGRAAVGAIEPTGEAVERLDDLRGHAAALLDPHDPHAVRRAGGWVLGP
                     LPEVTAAPTPAIRAAARRVRRPGGQVLGLTNGHRLVAVLGTEPELDGLAGELLTAAAG
                     AGTLLVAGEGSGLADRVGASGSLPGGTHLAGAVRELQAQGHVVALVAARGAAALAAAD
                     CGIGLLREGFDPPWGADLLCGPGLAQACRILDAAAA"
     gene            complement(1740..2501)
                     /locus_tag="VLC50_03280"
     CDS             complement(1740..2501)
                     /locus_tag="VLC50_03280"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HSF26519.1"
                     /translation="MGRFDDKTAVITGGASGIGLATATLLAQEGAYVVIAGRDTVRGA
                     AAAAVLGDRGEFVQTDVGQDRQVAELAALAAKRTGHIDYWFNNAGYEGPVGPFDLWTE
                     ENITELLDTNIKGVLSGLRHAAANIGDGGGVIVNTASFIGTRHHVPIAVAYGATKAAV
                     ISASASAAVALEPAGVRVYALCPWIIDTPMLERFIGSTTTAAKQDFAANFNPSGRLAT
                     PGDIAVAFADLVEGRLDVPNGGAFLVDAGAVVTPI"
     gene            complement(2503..3021)
                     /locus_tag="VLC50_03285"
     CDS             complement(2503..3021)
                     /locus_tag="VLC50_03285"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013674643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HSF26520.1"
                     /translation="MTIDTTVGNPLPAIIEGVERTLRNEPAKAVEVFAAHGTGTGRVS
                     SALTTRSFTLAADEPPPLGEDEAPNPVELALAGLASCQIVTYRVWAQKLGIELTDVDI
                     AVDGDLDFRGFFGLDDDIRPGFRDIRVEVRLRGPETPERYAELQRVVDQHCPMLDLLS
                     HATPVTTTVKVG"
     regulatory      complement(3255..3356)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(3473..3892)
                     /locus_tag="VLC50_03290"
     CDS             complement(3473..3892)
                     /locus_tag="VLC50_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014739157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DCC1-like thiol-disulfide oxidoreductase family
                     protein"
                     /protein_id="HSF26521.1"
                     /translation="MSTSSVGRPLLVFDGDCAFCTTSADLALRLFPDAFDAVPYQRAD
                     LPALRLSEAACRARLQWVDAAATRGAARPISAGAEAVTALLRAGGRARGGPLGATARL
                     VGAVAARPPLSWLAAAVYAVVAANRSRLPGGTPACRR"
     gene            3979..4491
                     /locus_tag="VLC50_03295"
     CDS             3979..4491
                     /locus_tag="VLC50_03295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HSF26522.1"
                     /translation="MRAVVVYESFQGNTKAVAEVIGQALSSSYDVTVLPVGQQATDAA
                     RGADLLVVGGPTWVHGPTSRKMREQNTDRIATPDSTGIEMGAREWIEALPEHAGSVAV
                     FDTRIKMPKVLTGAASKGLSRRLRERGARLVADPESFLVTKNDTLVEGELDRARAWGA
                     GLVSQRQPAD"
     gene            complement(4501..5145)
                     /locus_tag="VLC50_03300"
     CDS             complement(4501..5145)
                     /locus_tag="VLC50_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018162525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HSF26523.1"
                     /translation="MRSYLMEFIGTFGLVFTIGASLYAQSPLAPIGIGAVLMVLVYAG
                     GHISGAHYNPAITLGVLIRGRIRTSDVAPYVVAQLVGGVLGALVARVMANPGDLNALS
                     FSGREIVAALIAEFVFTFLLVFVMLNVATSKDHPNNSFYGLAIGFTVAAGAVAVGGLS
                     GAAFNPAVAIGVMVGGLLSWSTIWIYLLANLAAGAVAGYAFRVLNPHNLEPLAD"
     gene            5383..5772
                     /locus_tag="VLC50_03305"
     CDS             5383..5772
                     /locus_tag="VLC50_03305"
                     /inference="COORDINATES: protein motif:HMM:NF024636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2TM domain-containing protein"
                     /protein_id="HSF26524.1"
                     /translation="MTSMPTGDEPREPVREPAPDGPDLSARTPPPDQPPDPTQEAMRE
                     AEARRRDLARRKLQSRRDLSAHAVVFVVVNAFLVGIWAFTGRGYFWPGWVIGGWGIGL
                     ALNAWDVLLRRPVTEADVDAELRRRRD"
     gene            5888..7729
                     /locus_tag="VLC50_03310"
     CDS             5888..7729
                     /locus_tag="VLC50_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HSF26525.1"
                     /translation="MGHYQSNLRDIEFNLFEVFGRDAVLGAGPYAEVDGEVAREMLHE
                     VARLAEHELAASLLESDRTPPVFDPATHAVTLPAAFKRSYQAYLDAEWWRTDLPAELG
                     GTVVPPSLRWAIAELVLGANPAVHMYSSGYSFAKVLFLLGTPEQRRLAQYMVDGRWGA
                     TMVLTEPDAGSDVGAGRTKAVAQPDGTWHISGVKRFITSAEADIFDNVVHFVLARPEG
                     AGPGTKGLSLFIVPKFHVDLDTGALGARNGAFVTNVEHKMGLKVSTTCEVRFGEVDGI
                     PAVGTLLGEVHDGIAQMFRIIEYARMMVGTKAIATLSSGYLNALAYAKERLQGADLTR
                     MTDKTAPRVTILSHPDVRRSLMMQKAYAEGMRALVLYAATVQDTADLARHTGGYDDEA
                     ERLSDLLLPVVKGFGSERSYALLADALQVFGGSGYLQDYPLEQYIRDAKIDTLYEGTT
                     GIQGLDFFFRKIAKDQFRSITALAAQITETVKGGAGSDALSTERSLLGTALDDVQAIV
                     ATMGQAALASTTGAPEQVYAVGLNTTRLLMAAGDLTVGWLLLRQAEVALAALDRSDLS
                     AADAAFYQGKVAAARFFAAQVLPTLSAQRAIAESVDTSLMELPEAAF"
     gene            complement(7953..8606)
                     /locus_tag="VLC50_03315"
     CDS             complement(7953..8606)
                     /locus_tag="VLC50_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017616100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HSF26526.1"
                     /translation="MVVTVRAGRLQVLLWRRARPPYEGFWALPGGALGPDETLGASVA
                     RQLAAKVDVRELTHLEQLETRSEPDRDPRGRAVATAYLGLVPVQADPEVPADTAWHPV
                     DALPATAFDHGSIVASGVERLRGKLSYTNIGFALAPPVFTISELREVFRAALGHDVSA
                     TNLQRVLVRRDLLEPTGELAPPGRTGGRPATVYRFRARRLEVTDAFAVLRPPPPPGD"
     gene            8872..>9246
                     /gene="nadA"
                     /locus_tag="VLC50_03320"
     CDS             8872..>9246
                     /gene="nadA"
                     /locus_tag="VLC50_03320"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011291022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HSF26527.1"
                     /translation="MTTTSDAATGASAGAATVSSSEAPPRPRVSPRPLDQRFTHGADP
                     ALLATESDTWTEQQWADWHDEVRRLARERDAVILAHNYQDPAIQDVADHVGDSLALSR
                     LAASSEASTIVFAGVHFMAETAK"
BASE COUNT         1322 a         3363 c         3377 g         1184 t
ORIGIN      
        1 cgccgcggcg gcgtcgagga tgcggcaggc ctgggccagc ccgggcccgc acagcaggtc
       61 ggcgccccac ggcgggtcga acccctcgcg cagcagcccg atgccgcagt cggccgcggc
      121 cagcgcggcg gctccccgcg cggcgaccag ggcgaccacg tgcccctgcg cctgcagctc
      181 ccggaccgcg ccggccaggt gggtgccacc gggcagcgac ccactcgcgc cgaccctgtc
      241 ggccagcccg ctgccctcgc cggcgaccag cagggtgccg gccccggcgg cggcggtcag
      301 cagctcgccg gccagcccgt cgagctcggg ctcggtgccc agcacggcga ccagccgatg
      361 cccgttggtc aggcccagga cctgcccgcc cgggcggcgc acccggcggg cggcggcccg
      421 gatggcgggg gtgggtgcgg cggtcacctc gggcagcggg ccgaggaccc acccgccggc
      481 gcgtcgtacg gcgtgagggt cgtgggggtc gagcagcgcg gcggcgtgcc cgcgcaggtc
      541 gtcgagccgc tccaccgcct cgccggtggg ctcgatcgcg ccgaccgcgg cccgcccggt
      601 catcagcacc ggcgcgtcga ccaccaccgt gtcgacccgg tccagtcggc gcagcgccgt
      661 cgcgtccagc ggcacgatcc cgcggcgcca cagcagccgg cccagcgtcg aggcgaacac
      721 ctcgcgggtc aggcgggccg ccttgggggt gccgctgacg agcgcggcca cggcgtcgtt
      781 gtggttgcgg gtggctatcc aggtggtcgc ggcccccagc atcgccgcgg cggcggcgcg
      841 gtcgccgtac cgctccaccg gccccgccgg cataggcacc ggccgcggcg cgagggagag
      901 cggcgccgtg cgctcgtcgc tgggctgccc gtgcagctcc ggctcccgcg cgtgccagac
      961 cacgcgccgg gcggtgatct cgccgacctg cgccagccga tgcgtggcgt cgacgagcag
     1021 cccgagcggg ccctgggcga tgccctggct gaccgcgttg gcggtggcca gcagcaggtc
     1081 ggcggttgct gtgccgagcc ggcgctccgc ctgctcgcgc agccgcggcg tggagtcgac
     1141 cagcgagagc agcgaggcga cctcctcggt cagcgggccg cggcccagcg cccgcccgat
     1201 cgaggacagc cccagcccga gcgcgtcggc ggccagcgag gcgacctgcc ggtgcagcgg
     1261 ctcggcgtcg cccgggtgct cgggccgctc ggccgggaac gcctcctcgt gcagctcgtg
     1321 ggcctcttcg acgccctcga cgatgtcgac aaggtcgtcg acatcgatct gctcgccgtc
     1381 aaacgcgacc accacccggc ccagcaccgc gttgacctcg gcccagtgca cgccctcgac
     1441 gcggccgagg gcgtcctcga gccggcgagc gaacggctcg gtgccctcgc gatgggcccc
     1501 gcggacctcg atgtgggcgc ggtcgtggcc gacccaggcg cgccgcagcc ggcgagcccg
     1561 tcctggcagc agctcggcca gcacccgccc gacgcccagg ggtgcggccc gcagcgacgc
     1621 gaccggcgtc atccgcccag cctgcaaccg cgcgggggtg gcgcgcagcc agagccccga
     1681 gaagcacgga accggcggga cccccgtgcc cgccggttcc actgcccctt cgcgacgcgc
     1741 tagatcggcg tcaccaccgc gccggcgtcg acgaggaatg cacccccgtt gggcacgtcg
     1801 agccggccct cgacgagatc cgcgaaggcc accgcgatgt caccgggggt ggccagccgt
     1861 ccgctggggt tgaagttggc ggcgaagtcc tgcttggccg ccgtcgtcgt gctgccgatg
     1921 aaccgctcga gcatcggagt gtcgatgatc cacgggcaca gcgcatagac ccgtacgccg
     1981 gccggctcca gcgcgaccgc cgccgaggcg ctggccgaga tcactgcggc cttggtcgca
     2041 ccgtaggcca ccgcgatcgg cacgtggtgc ctggtgccga tgaacgacgc ggtgttgacg
     2101 atcaccccgc cgccgtcgcc gatgttcgcg gcggcgtgac gcaacccgga gagcaccccc
     2161 ttgatgttgg tgtcgagcag ctcggtgatg ttctcctcgg tccacaggtc gaacgggccg
     2221 accggcccct cgtagccggc attgttgaac cagtagtcga tgtgcccggt ccgcttggcc
     2281 gccaacgcgg ccagctcggc gacctgccgg tcttgaccca cgtcggtctg gacgaactcc
     2341 ccccgatcgc cgagtacggc ggccgccgcc gcgccgcgca cggtgtcacg gccggcgatc
     2401 acgacatagg caccctcctg ggccagcagc gtggccgtgg cgagaccgat cccgctcgcc
     2461 ccgccggtga tgacggcggt cttgtcgtcg aagcgcccca tttcagccca ccttcacggt
     2521 ggtggtgacg ggggtggcgt ggctgagcag gtcgagcatc gggcagtgct ggtcgacgac
     2581 ccgctgcagc tcggcgtagc gctcgggagt ctccgggccg cgcagccgca cctcgacccg
     2641 gatgtcgcga aagcccgggc ggatgtcgtc gtcgagcccg aagaagccgc ggaagtcgag
     2701 gtcgccgtcg accgcgatgt cgacgtcggt gagctcgatg ccgagctttt gggcccagac
     2761 ccggtaggtg acgatctggc aggaggccag cccggccagg gccagctcga ccgggttggg
     2821 ggcctcgtcc tcgcccagcg gcggcggctc gtcggccgcg agcgtgaagc tgcgggtggt
     2881 gagggcgctg gagacgcgcc cggtgccggt gccgtgcgcg gcgaacacct cgacggcctt
     2941 ggccggctcg ttgcgcagcg tccgctcgac gccttcgatg atggccggca gcggattgcc
     3001 gacggtcgtg tcaatggtca tgggaatgct ccgatcgggt agcggaaatg ggcgtgcgag
     3061 gaccatccgc ggcaccagaa ggaatgccga ggaaaggtga cgggtcagcg cgtcaggaat
     3121 gcaccgacag gcgtcgatgc cggtgagccg ggccgagctg tcggatcagt cggctggaca
     3181 gagggcgctg cggtagcgcc cgaagtcgat gcagcgacgg ctgaacagcc gtgcctgcac
     3241 gcggtgcacg cgatcactca tcaggtgtcc tcatcgattc tggcgttagc accggccccg
     3301 aagggtggtt gctgcgacgt caacgagcca gatctctcgg tcgctctgga tgaacgagca
     3361 gaattgcacc acgcattgtc gtctaaagtc aagaagcgcc cacgatgcga cattcgcggg
     3421 ccggcggcgc acccccaaga gcgggtgcgg aacggcgcgc cttgccagac ggtcagcgac
     3481 ggcaggcggg ggtgccgccg ggcagccgtg agcgattggc cgccaccacg gcatagaccg
     3541 cggccgccag ccacgacagc ggcgggcgcg cggccacggc gccgaccagc cgggcggtgg
     3601 cacccagcgg gccgccgcgt gccctgccgc cggcccgcag cagcgcggtc accgcctcgg
     3661 ctcccgcgct gatcggccgg gcggctccgc gcgtggcagc cgcgtcgacc cactgcagcc
     3721 gggcccggca cgctgcctcg gacaacctca gcgccggcag gtcggcccgc tggtagggca
     3781 cggcgtcgaa cgcgtcgggg aacagccgca gcgcgaggtc agcgctcgtc gtacagaatg
     3841 cgcagtcgcc gtcgaacacc agcagcgggc ggcccaccga cgaggtgctc accagggcga
     3901 gtatgcccgc tcaccgggga cgttcggcct ctgccaagcc acgagcggcg cgggtggact
     3961 cggggtgagg tgagtgcgat gcgcgcagtg gtggtttacg aatcgttcca gggcaacacc
     4021 aaggcggtgg ccgaggtgat cggccaggcg ctgtcctcgt cgtacgacgt cacggtgctg
     4081 ccggtcggcc agcaggccac cgacgcggct cgcggggccg acctgctggt ggtcggtgga
     4141 ccgacctggg tccacgggcc gacgtcgcgc aagatgcgcg agcagaacac cgaccggatc
     4201 gccacgcccg actcgaccgg gatcgagatg ggcgcccggg agtggatcga ggcgctgccc
     4261 gagcacgccg ggtctgtggc cgtcttcgac acccggatca agatgccgaa ggtgctgacc
     4321 ggtgcggcct cgaaggggct gtcccgtcgg ctgcgcgaac gcggggcgcg gctggtcgcg
     4381 gacccggaga gcttcctggt caccaagaac gacacgctcg tcgagggcga gctcgaccgg
     4441 gcgcgggcct ggggcgccgg gctggtctcc caacgccagc cggccgacta ggccgggcgg
     4501 tcagtcggcg agcggctcga ggttgtgcgg gttgagcacg cggaacgcgt agccggccac
     4561 cgctccggcc gcgaggttgg ccagcaggta gatccagatc gtcgaccagc tcagcagccc
     4621 gccgaccatc acgccgatcg ccaccgccgg gttgaacgcg gcaccgctga gcccgccgac
     4681 cgcgacggca ccggcggcca cggtgaagcc gatcgccaac ccgtagaacg agttgttggg
     4741 gtggtccttg ctggtcgcga cgttgagcat gacgaagacc agcaggaacg tgaagacgaa
     4801 ctcggcgatc agcgcggcga cgatctcgcg gcccgagaac gagagcgcgt tgaggtcgcc
     4861 ggggttggcc atcacgcggg ccaccagggc gccgagcacg ccacccacca gctgggcgac
     4921 cacgtagggc gccacgtcgg aagtgcggat gcgcccgcgg atcagcacgc ccaacgtgat
     4981 cgccgggttg tagtgggcgc cggagatgtg cccgccggcg tagaccagga ccatgagcac
     5041 ggcgccgatg ccgatcgggg ccagcgggga ctgcgcgtac aacgacgcgc cgatcgtgaa
     5101 gaccagcccg aacgtcccga tgaactccat cagatagctg cgcacggacg ctcctcacca
     5161 ggcgacaacg accaccgtca tcgcagcgag gataggcgac tggcgatcac gaggccggga
     5221 tacacgccgt ctcacgctgc ggtcagctcg gtccgtacgg cgccacgcgg ccgcgcgctg
     5281 actaggcgtg cggtcccggg cgccacggga ccttcgactc cgtgccaggc cccgaacgcc
     5341 gtacgacgct gcaagcacac gcagagtgga ggggagccgg cgatgacctc gatgccaacg
     5401 ggggacgagc cgcgcgagcc ggtccgagag ccagcgcccg acgggccgga cctgtctgcg
     5461 cgcaccccac cgcccgacca accgccggac ccgacgcagg aggcgatgcg ggaggccgag
     5521 gcgcgccggc gtgacctggc ccgccgaaag ctgcagagtc gccgtgacct gtccgcgcac
     5581 gccgtggtct tcgtcgtggt caacgcgttc ctggtcggga tctgggcgtt caccggccgc
     5641 ggctacttct ggcccggctg ggtgatcggc ggctggggca tcggcctggc gctcaacgcc
     5701 tgggacgtgc tgctgcgccg cccggtcacc gaggccgacg tcgacgccga gctgcgccgt
     5761 cgccgcgact gaccgcgaca cgaccacgcc cttgtgaggc gacccagcgg ctcaacccct
     5821 gtcggaggtg cgccgaaggt tactggcggg tagcatcctt tgttactcgc cagtaggagg
     5881 cacgacgatg ggtcactacc agagcaacct gcgcgacatc gagttcaacc tcttcgaggt
     5941 gttcggccgc gacgcggtcc tcggcgccgg tccctatgcc gaggtcgacg gcgaggtggc
     6001 ccgcgagatg ctgcacgagg tggcccggct ggccgagcac gagctggccg cctcgctgct
     6061 cgagtccgac cgcaccccgc cggtcttcga cccggccacc cacgcggtga ccctgccggc
     6121 ggcgttcaag cgcagctacc aggcctacct cgacgccgag tggtggcgca ccgacctgcc
     6181 ggccgagctg ggcgggaccg tcgtaccgcc gtctctgcgc tgggcgatcg ccgagctggt
     6241 gctcggcgcc aaccccgcgg tgcacatgta ttcgtcgggc tactcgtttg ccaaggtgct
     6301 gttcctgctg ggcacccccg agcagcgccg gctggcccag tacatggtcg acggccgctg
     6361 gggcgcgacc atggtgctga ccgagcccga cgccggctcc gacgtgggcg ccgggcggac
     6421 caaggcggtg gcccagcccg acggcacctg gcacatcagc ggcgtcaagc ggttcatcac
     6481 ctccgccgag gccgacatct tcgacaacgt cgtgcacttc gtgctggccc gccccgaggg
     6541 cgccgggccg ggcaccaagg ggctctcgct gtttatcgtg cccaagttcc acgtcgacct
     6601 cgacaccggg gcgctgggcg cgcgcaacgg cgcgttcgtc accaacgtcg agcacaagat
     6661 ggggctgaag gtctccacca cctgcgaggt ccgcttcggc gaggtcgatg gcatccccgc
     6721 ggtcggcacg ctgctcggcg aggtgcacga cggcatcgcg cagatgtttc ggatcatcga
     6781 gtacgcccgg atgatggtcg gcaccaaggc gatcgcgacc ctgtcgtcgg gctacctcaa
     6841 cgcgctggct tacgcgaagg agcggctgca gggcgccgac ctgacccgga tgaccgacaa
     6901 gacggctccc cgggtgacga tcctgtcgca tcccgacgta cgacgatcgc tgatgatgca
     6961 gaaggcctac gccgagggca tgcgcgcgct cgtgctctac gcggcgaccg tgcaggacac
     7021 ggccgacctg gcccgacaca ccggcggata cgacgacgag gccgagcggc tgtccgacct
     7081 gctgctgccg gtggtcaagg gcttcgggtc ggagcgctcc tacgcgctgc tggccgacgc
     7141 gctgcaggtc ttcggcgggt cgggctacct gcaggactac ccgctggagc agtacatccg
     7201 cgacgccaag atcgacacgc tctacgaggg caccacgggg atccagggcc tcgacttctt
     7261 cttccgcaag atcgccaagg accagttccg gtcgatcacc gcgctggccg cgcagatcac
     7321 cgagacggtc aagggcggcg cggggtcgga cgccttgtcg accgagcggt cgctgctggg
     7381 caccgcgctc gacgacgtgc aggcgatcgt ggccacgatg gggcaggccg cgctcgcgtc
     7441 gaccaccggc gcgccggagc aggtctacgc cgtcgggctc aacaccaccc ggctgctgat
     7501 ggccgccggt gacctgaccg tcggctggct gctgctgcgc caggccgagg tggcgttggc
     7561 cgcgctcgac cgctctgacc tctcggcggc cgacgcggcc ttctaccagg gcaaggtggc
     7621 cgcggcccgg ttcttcgccg cccaggtgct gccgacgctg tcggcccagc gggcgatcgc
     7681 cgaatccgtc gacacctcgc tgatggagct gcccgaagcc gcgttctgag cgcgcctgcc
     7741 cgacaccccc gccccggcct cctcagaacg tcgacgttcg cgacgcgaca cgccggcgtg
     7801 tctgtcgcgg acgtcgatgt tgtgggcagc gcgcctgctc gccgccggag gccgtcgtac
     7861 gacctgctct tgcgggccca ctaggcaagg cgttcagtca cttggcgagg cgtgcacgcc
     7921 ttgccaagtg gacgaacgcc cacttggcgt ggtcaatccc cagggggcgg gggcgggcgg
     7981 aggaccgcga aggcgtcggt cacctcgagc cggcgtgccc ggaaccggta gaccgtcgcc
     8041 gggcgcccgc cggtccggcc cggcggggcc agctcgccgg tcggctcgag caggtcgcgg
     8101 cggaccagca cccgctgcag gttggtcgcc gacacgtcgt gcccgagcgc ggcccggaac
     8161 acctcgcgca gctcggagat ggtgaacacc ggcggggcca gcgcaaagcc gatgttggtg
     8221 taggagagct tgccgcgcag ccgctccacc ccagaggcga cgatcgagcc gtggtcgaac
     8281 gcggtcgccg gcagcgcgtc gactgggtgc caggcggtgt cggccggcac ctccgggtcg
     8341 gcctgcaccg gcaccagccc gaggtaggcg gtggccaccg cccggccgcg ggggtcgcgg
     8401 tcgggctcgc tgcgggtctc cagctgctcg aggtgggtca gctcgcgcac gtcgaccttg
     8461 gccgccagct ggcgggccac cgaggcgccc agcgtctcgt cggggccgag cgcgccgccg
     8521 ggaagggccc agaagccttc gtacggcggt cgggcccgcc gccacagcag cacctgcagc
     8581 cggccggcgc gcacggtgac gaccacggcg agcgcttcgt ggcgataggc gccgaatgcg
     8641 cggggggtgc gagggctcgg ctcgctcgtc ggtccgccgc tgcgcccggc ggacggcccg
     8701 ccggggcgcc cggtgcccgg cccgttgacg cgcccggtgg tcggcccgcc ggcgcttgcg
     8761 ccgtacgcgc tcgaactgcc ctccaccgac accgtgccca ccccggaatg ctaatctgct
     8821 caccgcagtt ttcgactcta ggtagaaaac tcggcaggca ggagagccgc catgaccacc
     8881 acgtcggacg ccgccaccgg cgcgagcgcg ggcgccgcca ccgtctccag ctctgaggca
     8941 ccgccccggc cccgcgtgtc gccccgcccg ctcgaccagc ggttcaccca cggcgccgac
     9001 ccggccctgc tggccaccga gagcgacacc tggaccgagc agcagtgggc cgactggcac
     9061 gacgaggtac gccggctggc ccgcgagcgc gacgcggtga tccttgccca caactaccag
     9121 gacccggcga tccaggacgt ggccgaccac gtcggcgact cgctggcgct gtcgagactg
     9181 gccgcgagca gcgaggcctc gacgatcgtg ttcgccggcg tgcacttcat ggccgagacc
     9241 gccaag
//
LOCUS       DATIBB010000091        11062 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_311101, whole genome shotgun sequence.
ACCESSION   DATIBB010000091 DATIBB010000000
VERSION     DATIBB010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 11062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11062
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_311101"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..527
                     /locus_tag="VLC50_03325"
     CDS             <1..527
                     /locus_tag="VLC50_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003950081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HSF26528.1"
                     /translation="SGAVGAMSGPLHGGAPARVLGMLEQVERTGDARAWVRGALDRGE
                     RLMGFGHRVYRAEDPRARVLRRTAQELGAPRYEVAAALEQAALAELHERRPDRVLATN
                     VEFWAAIVLDFAGVAPHQFTSMFTCARTAGWSAHILEQKRLGKLVRPSAVYVGPGERR
                     PEDVEGWEPDLLTH"
     regulatory      614..707
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            1022..2395
                     /locus_tag="VLC50_03330"
     CDS             1022..2395
                     /locus_tag="VLC50_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005263886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSF26529.1"
                     /translation="MSQSQSHGTAIQRRSPIGRVALGSFIGTSIEWYDFFLYGTAAAL
                     VFSELFFPNVSPAVGLIAAFGTYAVGFLARPLGGIIGGHLGDRIGRKAVLVASLTIMG
                     AATAAIGLLPSYDQIGLWAPALLLVLRLTQGLGVGGEWGGAALMTVEHAPAARRGLFG
                     SFTQIGVSAGMLLSVGAFAVVRNSVDHATFLAWGWRVPFLASTILVAIGLWIRLKLEE
                     TPQFARLADEGGTSARPVVTALRDERKAVLLTTGMRLSQNAVYYIYTVFGLAYVARSL
                     GKDTNIGLISIVLASALGLVSVPLWAALSDRIGRRPVYLLGSVASAIFIFPFFLLADS
                     GNPVLIVLAIVIGLNGFHDAMYGPQAAFFSEMFTTGVRYSGASLGYQIGSVLAGGFSP
                     LIATALLAVGGGRPWLVCGYFLLLSSITTVCAYLAPETVRGEIGDAAPGRSRLARVLV
                     AEALATT"
     gene            2427..4013
                     /locus_tag="VLC50_03335"
     CDS             2427..4013
                     /locus_tag="VLC50_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015890872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HSF26530.1"
                     /translation="MNTFSLPTPPSQSLTLAHLVWRMSHVHHRSQIVTSGRTRLTFGE
                     SADRVRSLAGALVGLGLQRGDVVATLAWNTHEHLETMLAAPLAGAVVNALNPRLGAGA
                     LLALLGKATPKALVVAAGMLDPADPVSTVVRGVVDELVREGAVVVAVGAGPTTTPVIG
                     YEDFLHSGAGRTLPVVTEDDCAFLIHTGGTTGLPKSYAVSHRAAMLHALAQSGVDASG
                     LSRADRLLPLAPLFHVNSWGLPFTALLTGCSIVLPGRDLNAAWIRRVLDEEAVTVGAG
                     VPTLWYDVIEHVRSQGGAPTHLREVITGGSPVPASLVSGIQRDLGASVATAWGMTETM
                     ACTTYERELPAQRVGRPIPLVELRLVSTPDDLETSPPSGLVEVRGAFVVGTRRAPQAE
                     GWVATGDLASVDEDGRLRIVDRVKDLIKSGGEWIPAGDLEAALNAHPAVSLAAVVAAP
                     HPRWMERPVAYVTLLAGQRIGPQELREHLRGLMPSWWLPDRIHVVPELPRTGVGKIDK
                     SLVRTWASHDLTDDAEGAHS"
     gene            4010..4867
                     /locus_tag="VLC50_03340"
     CDS             4010..4867
                     /locus_tag="VLC50_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015890871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HSF26531.1"
                     /translation="MTDLAATTREQSGLGQLIKSEHKGEITTITIDRPAKLNALSGAL
                     MTQLSHALTAAESDDDTKVVVLTGAPSAFSVGYDISEEVASGLARPEDWHRQLSSNVA
                     LTMQVWSLSKPVVGAVDGWCLAGGCELAMACDILIAADTAQFGEPEIRFGSGPVTLLM
                     PFILGQKRTNELLFTGDVIDATTAERLGLINRVVPAPELLATAEQLARKIALTPLPVL
                     RLTKLALNRAYEAMGLRNAVSANLDLAAVLNAAHSKEHSEFGELVATQGLRSALAWRD
                     ARYAPFEQA"
     gene            4896..6881
                     /locus_tag="VLC50_03345"
     CDS             4896..6881
                     /locus_tag="VLC50_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011599600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HSF26532.1"
                     /translation="MLTSSTLPTSAEGGQRTPPVWRPGPDELKRSRLAAFCRERGFAD
                     PAALDAAARRDPAWFWQAVTDWLDLDWQSAPTGPVDQLEVGHQSRWFPGGSFNLADNA
                     VDRWVRRGRGDQPALVWEREDGTRGTLTFTELAAQVDRVGHGLLAIGVRFGDRVGIQL
                     PMVAEAAVTALACAKIGAIAVPVFSGFGPTAVADRLRLAGASVHVVADGYARRGRVVP
                     LRATVAEALAAAPSVQRTVVVPLAGQPVAGPELPGEIAWADLPAPADATTLLPAAVCP
                     TEHPLFIAFTSGTTGVPKGVVLGHAGFAVKAASDVAFCFDIGEGDVATWITDPGWIMS
                     PIIVFGGLLAGATVALYDGALDHPGCDRLWTLVRELGITMLGVSPTLVRSLMTAGPLD
                     EAPDLGPLRVLASSGEPWTPDAFSWLFDRVTGGRLPIINYSGGTEVSGAIVSNTTITP
                     IHACGFAGPVPGMAADVVDDASDPIEAGIGELVLRAPSPGMPLGFWGDPDRYHATYWA
                     RWDNVWRHGDWVEVDPDGVWFIRGRSDDTIKVAGKRLGPAEVESVVNAFPAVTESAAI
                     GVPDPVKGQAVVVFAVLDPREDADEEALRAETVAALTAQLGKPLKPKAVHFVDALPHT
                     RSGKILRRVVRAVHLGEDVGTAAVDDAAALDAVRNAR"
     gene            6878..8038
                     /locus_tag="VLC50_03350"
     CDS             6878..8038
                     /locus_tag="VLC50_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010529558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HSF26533.1"
                     /translation="MSLRATAAIAGVAELPPARTTPGQTTLGMIGRVAAAAVADAGLE
                     PAAIDGLLVGPQVGETPQHVPATIAEYLGLAPTMANVVDLGGASGPGMVWRAAAAIAA
                     GMCETVLCVLGNSRDPAVVRRSPNRNPIREFDVPYGGSGANQAYAMLATRHMAEFGTT
                     AEQLAHVPVFERANAALNPDAVFHGRPLSVADVLASPLVMAPLHLLEIVMPCGGGAAV
                     VVTSAARARTLAQPPVPLLGAGEKLTHRALSQAPSLTTSPLAAAIASAYAMAGVGPAD
                     LDLLSLYDCYSIVVALTLEDAGLVPKGQVGPWLLDQDFSATGNLPLNTHGGQLACGQA
                     DLAGGMGHVVEAVRQLRGQAGARQVPDAEVALVTGNGATLSEEVALVLGAAA"
     gene            8035..8514
                     /locus_tag="VLC50_03355"
     CDS             8035..8514
                     /locus_tag="VLC50_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009156890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HSF26534.1"
                     /translation="MSGERGADGARVPRPPMLSTPATAPYWAAAAVGRLLLQRCRSCG
                     RLQHYARTLCAGCWSEDLDWVEAAGTGTVWTLTVAHVPGHPAWAAAAPYVLAIVELDE
                     GPRLLTNVVGVPPQSVAVGQRVRLVRHTDSCPEGDPALLQFTPLESVNPNEIKEIQP"
     gene            8511..9881
                     /locus_tag="VLC50_03360"
     CDS             8511..9881
                     /locus_tag="VLC50_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013862276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSF26535.1"
                     /translation="MTTIVDPVTPPGDAAPPGSPEAAAVAATRRRAKLASFVGTSIEW
                     YDYYLYGTSAALVFNKLFFPEFSALTGTLLALLTFSAGFIARPLGGIVMGHFGDRIGR
                     KAMLVTSLLVMGVATTLIGVLPTYDAIGLWAPLLLLLMRIAQGFGVGGEWGGAVLLAV
                     EHAPAHRRGVFGAWPQVGVPVGLLLSNGIFITLTVTTSSEAFLAWGWRLCFLLSAPLV
                     LFGLLIRLRIEETPDFQAVKDAGRVHKVPLLVVLRRQWQEVLLAIGIRLAQNAIFYII
                     TVFALTYASTVLDVPKSIVLGGVLIGAGVSLVNLLFVSWLSDEYGRRRVYLAGAIGWA
                     LFAFPFFWLLDTKTTVLMWLAFVVGLALHDTMYGPQAAYFSELFDADVRYTGASLGYQ
                     VASLIAGAVSPVVAVLLLDVADNKPWPVALYVVGLSLITVVATALAPETHRGNRHRPQ
                     EVARGR"
     gene            9871..11040
                     /locus_tag="VLC50_03365"
     CDS             9871..11040
                     /locus_tag="VLC50_03365"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016697224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HSF26536.1"
                     /translation="MAAETGTRTPVLVSSARTPVGRFGKGLAGVAAVDLGATAVAAAV
                     SRAGGLVPDQVLLGNVVQAGNGQNPARVAATRAGLGLGVPAITLNDVCLASMSAAGLA
                     ATLIRAGEIETAVVGGFESMSRALHGVQVRAAGKVGDGAMVDLLVHDGLWCALSERGM
                     GELSDATNDALGIDRVTQDELALASHQRAHAATTGGRLAEEIVPVAGVDLLTDEGIRP
                     ATTPAGLAALAPAFRPGGTITAGNASQLSDAGAAGVVTTLDLARAHGLEPLVEIVGRA
                     VIAGPDPTLHLRPADAARVLLKRNGLTVADIGLWEINEAFAGVVVASMRDLGLDPARI
                     NVNGGAIALGHPLGATGFRMVATLAREMRRTGVSHGVAAICGGGGQGQAILLRLV"
BASE COUNT         1466 a         3975 c         3905 g         1716 t
ORIGIN      
        1 tgtcgggcgc ggtcggcgcg atgtcggggc ctttgcacgg cggcgccccg gcccgggtgc
       61 tcggcatgct cgagcaggtc gagcgcaccg gcgacgcccg ggcctgggtc cgcggcgccc
      121 tcgaccgcgg tgagcggctg atgggtttcg gccaccgggt ctaccgcgcc gaagacccgc
      181 gcgctcgcgt gcttcgccgt acggcgcaag agctgggcgc gccccgctat gaggtggccg
      241 cggcgctcga gcaggccgcc ctggccgagc tgcacgagcg gcgccccgac cgggtgctgg
      301 ccaccaacgt ggagttctgg gccgcgatcg tgctcgactt cgccggggtc gcgccgcacc
      361 agttcacgtc gatgttcacc tgcgcgcgca ccgcgggttg gagcgcgcac atcctcgagc
      421 agaagcggct gggcaagctc gtgcgcccgt cggcggtcta cgtcggaccc ggcgaacggc
      481 gcccagagga cgtcgagggc tgggagcccg acctgctcac ccactgactc ttcgccgagt
      541 gcgagtccac gtgagtgttc ggatggtgga cggagtgtgc gcctcgcggg cggggtgccc
      601 tattcttcgg cccgttctat caagagcggc cgagagacct ggctcgtcga cgtcgcagca
      661 accccctgcc ttgtgggtgc gggtgctaaa gccaggaacg atggaggaca ccgtgcacgc
      721 ccgtgcgctc gcatcacccc cgtggagcca tgccccattc ctgaggcgct tcatcgacct
      781 gggacgcacg cacgccgcga tgtgtcgacg ctcgccaagc atgagctgag ctgattctcc
      841 ggcgcggaca tcgagtccac gccgcagctt ctccgcctgg gactcgtcca cgggcttctt
      901 tcggcatgca gaagtgccgc attcacgccc gccgctagcg gcgtccgtgt catgccgtgc
      961 ttgcaggcct gacgagatca ctcaccttcg ccatcgggcg aaccacgtct tgaggggatc
     1021 catgtcccag tcacaatcac acggtacggc catccagcgg cgttcaccga tcggccgagt
     1081 cgcgctcggc agcttcatcg gaacatcgat cgagtggtac gacttctttc tttacggcac
     1141 tgccgcggct ctcgtgttca gcgagctctt ctttcccaac gtgagcccgg cggtcggcct
     1201 catcgctgca ttcggcacct atgcagtggg attcctggcc cgcccgctgg gcgggatcat
     1261 cggagggcac ctcggcgacc gaatcggccg caaggccgtg ctcgtcgcct cgctcacgat
     1321 catgggtgcc gccaccgcgg ccatcgggct gctgcccagc tacgaccaga tcggtctgtg
     1381 ggcgccggcc ctgctgctgg tgctgcgtct gacgcagggt ctcggcgtcg gtggtgaatg
     1441 gggcggagcc gcgctgatga cggtcgagca cgcaccggcc gcccgtcgtg gcctcttcgg
     1501 cagcttcaca cagatcggcg tgtcggcagg catgctcttg tcggtcgggg cattcgctgt
     1561 cgttcgcaac agcgtcgacc acgcgacatt cttggcgtgg ggctggcgag tcccgttcct
     1621 cgccagcacc attcttgtcg cgatcggcct gtggatccgg ctcaagctcg aggagacacc
     1681 gcagttcgcc cgcctcgccg acgagggggg cacctcggcc cggccggtcg tcaccgctct
     1741 gcgtgacgag cggaaggcgg tgctgctcac gactggaatg cggctttcac agaatgccgt
     1801 ctactacatc tacaccgtgt tcgggctggc atacgtcgca cggagcctcg gcaaagacac
     1861 caacatcggc ctcatctcga tcgtgttggc atccgcgctc gggttggtca gcgtgccgct
     1921 gtgggccgcc ctgtccgacc ggatcgggcg ccgcccggtc tacctgctgg gatccgtcgc
     1981 cagcgcaatc ttcattttcc ccttcttcct cctggctgac tccggcaacc ccgtgctcat
     2041 cgtcctcgcg atcgtgatcg ggctcaacgg cttccacgac gcgatgtatg ggccgcaggc
     2101 ggccttcttc tccgagatgt tcacgaccgg ggtccgctac agcggggcct ccctcggcta
     2161 tcagatcggg tcggtccttg cgggcggctt ctcgccgctg atcgccacag ccctgctggc
     2221 tgtgggaggc ggccgcccgt ggctggtgtg cggctacttc ctcctcctgt catccatcac
     2281 cacggtctgc gcctacctgg ctcccgagac ggtcaggggc gagatcggtg acgcggctcc
     2341 tggccggtcc aggctcgcgc gcgtcctcgt cgccgaagct ctggcaacga cgtgagccgc
     2401 tcgcggacga ggacttatcc aacggcatga acaccttctc gctgccgaca ccaccgagcc
     2461 agtcgctaac cctcgcgcac ctggtgtggc gcatgtcgca cgtacaccac cggtcgcaga
     2521 tcgtcacctc ggggcggacg cggctgacct tcggtgagtc cgcagaccgg gtgaggtcgc
     2581 tagccggcgc gctggtcggc ctaggcctgc agcgcggtga cgtcgtggcc acgctggcgt
     2641 ggaacaccca cgagcacctg gagacgatgc tggcagcacc actggcaggt gcggtggtca
     2701 acgcgctcaa cccgcggctg ggtgccgggg cattgttggc actcctgggc aaggccaccc
     2761 ccaaggcact cgtcgttgcg gcgggcatgc tggatcctgc cgacccggtg agcaccgtgg
     2821 tgcgtggcgt cgtcgacgag ctagtgcgcg aaggtgccgt cgtcgtggcg gtcggcgcgg
     2881 ggccgaccac gacgccggtg atcggctacg aggacttcct gcattccggc gcggggcgga
     2941 cgctgccagt cgtcaccgag gacgactgcg ccttcctcat ccacaccggc ggcacgacgg
     3001 ggctgccgaa aagctacgcg gtcagccacc gggcggcgat gctgcacgcc ttggcgcagt
     3061 cgggcgtcga cgcgtcgggg ctctcccggg ccgaccggct gctcccgctc gcgccgctgt
     3121 ttcatgtcaa ctcctggggc ctgccgttca cggccctgct taccgggtgc tcgatcgtgc
     3181 tgccgggccg ggacctgaac gccgcgtgga tcaggcgggt gctcgacgag gaagccgtga
     3241 cggttggggc tggtgtcccg accctctggt acgacgtcat cgagcacgtc cgcagccagg
     3301 gtggcgcacc gacccacctg cgcgaggtca tcaccggtgg cagccccgtc ccagcctcac
     3361 tcgtcagcgg gatccagcgc gatctcggag caagcgtggc caccgcctgg ggcatgaccg
     3421 aaaccatggc gtgcaccacc tacgagcgcg agctgccggc tcaacgggtg ggtcggccga
     3481 ttccgctggt cgagctgaga ctcgtgagca cgcccgacga tctggagacg tcaccgccct
     3541 ctggcctcgt ggaggtgcgc ggtgcgttcg tcgtcgggac gcggcgtgcg ccccaggccg
     3601 agggctgggt ggccaccggc gacctggcca gcgtggacga agacggccga ctgcggatcg
     3661 tcgaccgggt caaggacctg atcaagtccg gcggcgaatg gatccctgcg ggggatcttg
     3721 aggccgcgct caacgcacac cctgcagtgt ccttggccgc cgtcgtcgcc gcaccgcacc
     3781 cgcggtggat ggaacgaccg gtggcctacg tcaccttgct tgcgggccag cgcattggac
     3841 cgcaggagct gcgcgagcac ctgcgaggcc tcatgccctc atggtggctg ccggaccgca
     3901 tccacgtcgt cccagagctg ccccggaccg gcgtcggaaa gatcgacaag tcgcttgtcc
     3961 ggacctgggc ctcacacgac ctgaccgacg acgccgaagg agcgcattcg tgaccgacct
     4021 agccgcaacc acacgagagc aatcaggtct gggccagctc atcaagtccg agcacaaagg
     4081 cgagatcacc accatcacga tcgaccgccc ggccaagctc aatgcgctca gcggtgctct
     4141 gatgacgcag ctgtcgcacg ccttgaccgc ggccgagtct gatgacgaca ccaaggtggt
     4201 cgtgctgacc ggcgcgccgt cggcgttcag cgtcggttac gacatctctg aagaagtcgc
     4261 gtccgggctg gcccgaccgg aggattggca tcgccagctg tcgtccaacg tggcgttgac
     4321 gatgcaggtg tggtcgttga gcaaaccggt ggtcggtgcc gtagacggct ggtgcttggc
     4381 cggtggatgc gagctggcga tggcgtgcga catcctcatc gccgccgaca ccgcgcagtt
     4441 cggtgaacca gagatccggt tcgggtcggg ccccgttacg ctgctgatgc cattcatcct
     4501 cggccagaag cgcaccaacg agctgctgtt caccggcgac gtcatcgacg ccacgaccgc
     4561 agaacgcctc gggctgatca accgcgtcgt tcccgcgccc gagctgcttg ccacagccga
     4621 gcagctcgcg cgcaagatcg ccctcacgcc gctgccggtg ctgcggctga ccaagctggc
     4681 cctcaaccgc gcctacgagg cgatggggct acgcaacgcc gtcagcgcca acctcgacct
     4741 ggccgcggtc ctcaacgccg cgcacagcaa ggagcacagc gagttcgggg aactcgttgc
     4801 cacgcaaggc ctgcgctcgg ctctcgcatg gcgcgacgcg cgctacgcgc ccttcgagca
     4861 agcctgaccg acaaccattt cttctggaga aacgcatgct cacgtcatca accctgccca
     4921 cctccgccga aggcgggcag cgcacaccgc cggtctggcg gcccgggccc gacgagctca
     4981 agcgcagccg actggccgcg ttctgccgcg agcgaggctt cgccgacccg gcggcgctgg
     5041 acgcggcggc ccggcgtgac ccggcctggt tctggcaggc ggtcaccgac tggctcgacc
     5101 tggactggca gagcgcgccc acgggccctg tcgaccagct ggaggtgggc caccagtcgc
     5161 ggtggttccc gggcgggagc ttcaacctgg ccgacaacgc ggtggaccgg tgggtgcgcc
     5221 gcggccgcgg cgaccagccg gccctggtct gggagcgcga ggacggcacg cgcggcacgc
     5281 tcacgttcac cgagctggcc gcgcaggtcg accgggtcgg ccacgggctg ctcgcgatcg
     5341 gagtgcgctt cggcgaccgg gtcggcatcc agctgccgat ggtcgccgag gcggcggtga
     5401 ccgcactggc ctgcgccaag atcggcgcca tcgcggtgcc ggtgttctcc ggcttcgggc
     5461 ccaccgcggt ggccgaccgg ctgcgcctgg ccggggccag cgtgcacgtg gtcgcggacg
     5521 gctacgcacg ccgcggccgg gtcgtcccgc tgcgcgccac ggtcgccgag gcgctggccg
     5581 ccgcaccctc ggtgcagcgc acggtcgtcg taccccttgc tggtcaaccg gttgcgggtc
     5641 ccgagctgcc cggtgagatc gcgtgggcgg acctgcccgc gcccgccgat gccacgaccc
     5701 tgctgccggc ggcggtctgc ccgaccgagc acccgctgtt catcgcgttc acctcgggca
     5761 ccaccggcgt gccgaagggc gtggtgctcg ggcacgccgg cttcgcggtc aaggccgcca
     5821 gcgacgtcgc gttctgcttc gacatcggcg agggcgacgt ggcgacctgg atcaccgacc
     5881 cgggctggat catgagcccg atcatcgtgt tcggcgggct gctggccggt gccaccgtgg
     5941 cgctctacga cggggccctc gaccaccccg gctgcgaccg gctctggacg ctggtgcgcg
     6001 agctcggcat cacgatgctc ggcgtctccc ccactctggt ccgctcgctg atgaccgcgg
     6061 gcccgttgga cgaggcgccg gacctcgggc cgttgcgagt gctggcctcc agcggcgagc
     6121 cgtggacccc cgacgcgttc tcgtggctgt tcgaccgggt caccggcgga cggctcccga
     6181 tcatcaacta ctcgggcggc accgaggtct ccggggccat cgtctccaac accacgatca
     6241 cgccgatcca cgcctgcggc ttcgccggcc cggtgcccgg catggccgcc gacgtcgtcg
     6301 acgatgccag tgacccgatc gaggccggca tcggcgagct ggtgctgcgc gcgccctccc
     6361 ccggcatgcc gctgggcttc tggggcgacc cggaccgcta ccacgccacc tactgggccc
     6421 gctgggacaa cgtttggcgc cacggcgact gggtcgaggt cgaccccgac ggcgtgtggt
     6481 tcatcagggg tcgcagcgac gacaccatca aggtggccgg caaacggctc ggcccggccg
     6541 aggtggaaag cgtcgtcaac gccttcccgg cggtgacaga gtcggccgcg atcggcgtgc
     6601 ccgacccggt caagggccag gcggtcgtgg tgttcgccgt gctcgatccg cgggaagacg
     6661 ccgacgaaga ggcgctgcgg gccgagaccg tcgcggcgct gaccgctcag ctgggcaagc
     6721 cgctcaagcc caaggcggtg cacttcgtcg acgcgctgcc gcacacccgc agcggcaaga
     6781 tcctgcgtcg ggtggtgcgc gcggtgcatc tcggcgagga cgtcgggact gccgcggtcg
     6841 acgacgccgc cgcgctggac gccgtacgga atgcgcgatg agcctgcgcg ccaccgccgc
     6901 gatcgccggg gtcgccgagc tgcccccggc ccggaccacc cccggccaga cgacgctggg
     6961 catgatcggg cgggtcgcgg ccgctgcggt cgccgacgcc gggctcgaac ccgccgccat
     7021 cgacgggctg ctggtcggcc cgcaggtcgg cgagaccccg cagcacgtgc cggccacgat
     7081 cgccgaatac ctcggcctgg ctccgacgat ggccaacgtc gtcgacctgg gcggcgcgtc
     7141 cgggccgggc atggtgtggc gggccgccgc ggcgatcgcg gcggggatgt gcgagacggt
     7201 gctgtgcgtc ctgggcaaca gccgtgaccc cgcggtggtg cgccgctccc ccaatcgcaa
     7261 cccgatccgt gagttcgacg tgccctacgg cggctccggc gccaaccagg cctacgcgat
     7321 gctggccacc cggcacatgg ccgagttcgg caccaccgcc gagcagctgg cgcacgtgcc
     7381 cgtgttcgag cgggccaacg ccgcgctcaa tcccgacgcg gtgttccacg gccgcccgct
     7441 cagcgtcgcg gacgtgctgg cctcgccgct ggtgatggcg ccgctgcacc tgctcgagat
     7501 cgtgatgccg tgcggcgggg gcgcggcggt cgtggtcacc agcgcggcgc gggcccgcac
     7561 gctggcccag ccgccggtgc cgctgctcgg ggccggggaa aagctcaccc accgggcgct
     7621 gagccaggcc ccgtcgctga ccacctcgcc gctggccgcg gcgatcgcct ctgcctatgc
     7681 gatggccggg gtgggtccgg ctgacctcga cctgctctcg ctctatgact gctactcgat
     7741 cgtggtcgcg ctgaccctgg aggacgccgg gctggtgccc aagggccagg tcgggccgtg
     7801 gctgctcgac caggacttct ccgcgaccgg caacctgccg ctcaacaccc acggcggcca
     7861 gctcgcctgc gggcaggccg acctggccgg cggcatgggc cacgtcgtcg aggcggtgcg
     7921 ccagctgcgt gggcaggccg gggcccggca ggtgcccgac gcagaggtgg ccctggtcac
     7981 cggcaacggc gccacgctca gcgaagaggt cgcgctggtg ctcggagcgg cggcatgagc
     8041 ggcgagcgcg gcgctgacgg cgcacgcgtg ccccgaccgc cgatgctgag caccccggcc
     8101 accgccccct actgggcggc cgccgccgtg ggccggctgc tgctgcagcg ctgccgctcg
     8161 tgcgggcgcc tgcagcacta cgcccgcacc ctgtgcgccg gctgctggtc ggaggacctc
     8221 gactgggtcg aggccgccgg caccggcacg gtctggacac tgaccgttgc gcacgtgccc
     8281 gggcacccgg cgtgggcggc cgcggcgccc tacgtgctgg ccatcgtcga gctcgacgag
     8341 gggccgcggc tgctcaccaa cgtggtcggc gtgccgcccc agtcggtcgc ggtcgggcag
     8401 cgcgtccggc tcgtgcgcca caccgactcc tgtcccgagg gtgatcccgc cctcctccag
     8461 ttcaccccgc tcgaatccgt gaatcccaat gaaatcaagg agatccagcc atgaccacca
     8521 tcgtcgatcc ggtcacgcca cccggtgacg ccgccccgcc cggctccccc gaggccgcgg
     8581 cggtcgcggc cacccgccgc cgggcgaagc tggcgagctt cgtcggcacc tcgatcgagt
     8641 ggtacgacta ctacctctac ggcacctccg cggcgctggt cttcaacaag ctgttcttcc
     8701 cggagttctc ggcgctgacc ggcaccctgc tcgcgctgct gaccttctcg gccggcttca
     8761 tcgcccgccc gctcggcggc atcgtcatgg gccacttcgg cgaccggatc ggccgcaagg
     8821 cgatgctggt cacctcgctg ctcgtcatgg gcgtggcgac gacgttgatc ggtgtgcttc
     8881 cgacgtacga cgcgatcggg ctctgggccc cgttgctgct gctgctgatg cgcatcgcgc
     8941 agggcttcgg cgtcggcggc gagtggggcg gggcggtgct gctggcggtt gagcacgccc
     9001 cggcgcaccg gcgtggggtg ttcggcgcgt ggccgcaggt cggcgtcccg gtcgggctgc
     9061 tgctgtccaa cggcatcttc atcaccctga ccgtgacgac cagctcggag gcattcctgg
     9121 cctgggggtg gcggctgtgc ttcctgctct cggccccact ggtgctgttc ggcctgctga
     9181 tccggctgcg catcgaggag accccggact tccaggcggt caaggacgcc gggcgggtgc
     9241 acaaggtgcc gctgctggtg gtgctgcgcc gccagtggca ggaagtgttg ctggccatcg
     9301 ggatccggct cgcacagaac gcgatcttct acatcatcac ggtgttcgcg ctcacctacg
     9361 ccagcaccgt gctcgacgta cccaagtcga tcgtgctcgg cggggtgctc atcggcgccg
     9421 gggtcagcct ggtcaacctg ctcttcgtca gctggctctc cgacgagtac gggcggcggc
     9481 gggtctacct cgccggcgcg atcggttggg cattgtttgc gttcccgttc ttctggctgc
     9541 tcgacaccaa gaccacggtg ctgatgtggc tggcgttcgt ggtcgggctg gcgctgcacg
     9601 acacgatgta tggcccgcag gcggcctact tctccgagct cttcgacgcc gacgtgcgct
     9661 acaccggcgc ctcactgggc taccaggtcg cctcactgat cgccggcgcg gtctccccgg
     9721 tggtcgcggt gctactgctc gacgtggccg acaacaagcc gtggccggtc gcgctctacg
     9781 tcgtcgggct cagcctgatc accgtggtgg ccaccgcgct ggccccggag acgcaccgcg
     9841 gcaaccggca ccgtccccag gaggtcgctc gtggccgctg agactggtac gcgcaccccg
     9901 gtgctggtgt cctcggcccg cacgccggtg ggcaggttcg gcaaggggct cgccggggtg
     9961 gccgccgtcg acctgggcgc caccgccgtg gccgcggcgg tctcgcgagc cggcggcctg
    10021 gtgcccgacc aggtgctgct cggcaatgtc gtgcaggccg gcaacgggca gaacccggcc
    10081 cgggtcgccg cgacccgggc cgggctgggc ctcggcgtac cggcgatcac gctcaacgac
    10141 gtgtgccttg ccagcatgag cgcggccgga ctggcggcga cgctgatccg cgccggagag
    10201 atcgagaccg cggtggtcgg cgggttcgag tcgatgagcc gggcgctgca cggcgtgcag
    10261 gtgcgcgccg cgggcaaggt cggcgacggc gcaatggtgg acttgctggt ccacgacggg
    10321 ctctggtgcg cgttgtccga gcgcgggatg ggcgagctct ccgacgccac caacgacgcg
    10381 ctcggcatcg atcgggtgac ccaggacgag ctggcgctgg ccagccacca gcgggcacac
    10441 gccgccacga cgggcgggcg gctggccgag gagatcgtgc cggtcgccgg cgtcgacctg
    10501 ctcaccgacg aggggatccg gccggcgacc acaccggccg gcctggccgc gctggccccg
    10561 gcgttccgtc ccggcggcac catcaccgca ggcaacgcgt cgcagctctc cgacgccggc
    10621 gcggccgggg tggtgaccac cctcgacctg gcccgcgcgc acgggctcga gccgctggtc
    10681 gagatcgtcg gccgggcggt gatcgccggc ccggacccga cgctgcacct gcgtccggcc
    10741 gacgcggcgc gggtgctgct caagcgcaac gggctcaccg tggccgacat cgggctgtgg
    10801 gagatcaacg aggccttcgc cggcgtcgtg gtcgcgagca tgcgcgacct cggcctggac
    10861 ccggcgcgga tcaacgtcaa cggcggcgcg atcgcgctgg gccatcctct gggagccacg
    10921 gggttccgga tggtggccac gttggcccgc gagatgcgcc ggaccggcgt gtcccacggg
    10981 gtcgcggcta tctgcggcgg cggcggccag ggtcaggcga tcctgctgcg cctcgtgtga
    11041 cctaggcaag gcgttgtggc ac
//
LOCUS       DATIBB010000092         4636 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_311173, whole genome shotgun sequence.
ACCESSION   DATIBB010000092 DATIBB010000000
VERSION     DATIBB010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 4636)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4636)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4636
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_311173"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            191..733
                     /locus_tag="VLC50_03370"
     CDS             191..733
                     /locus_tag="VLC50_03370"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019874461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HSF26537.1"
                     /translation="MAEQLFATLHTSKGDVTVELFPDFAPKTVRNFVELAEGTREWTH
                     PKTGAKTTDKLYDGTVFHRVIPGFMIQGGDPLGTGTGGPGYEFDDEPLPEKTFDRPYL
                     LAMANAGKRGGKGTNGCQFFITVAPTTWLNFKHTIFGEVADGDGRAVVDAIAAVPTGA
                     MDRPVEPVVIKSVTIERRAS"
     gene            750..1631
                     /locus_tag="VLC50_03375"
     CDS             750..1631
                     /locus_tag="VLC50_03375"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019432153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HSF26538.1"
                     /translation="MTDDPGTASPPAAVPTCYRHPGRETYIRCARCNRPICPDCMVPA
                     SVGFQCPECVREGNSTQRAPRTAFGGRLASGARVTMVLIGLNVLMYVAELALGRAFVN
                     DLALVGLAREGFLGPIVGVADGEWWRLVTATFLHDPGSFLHILFNMYALYVLGPPLEA
                     MLGHARFLALYLLSALGGTAASYTFSAPNSSSVGASGAIFGLFAAWIVLARKQRLDAR
                     PMLVLLGINLVLGFVIAGIDWRGHLGGMVTGAAVAAIMAYAPRGPRRTTWQVAGAVAV
                     LALVVLVDVWRTASLLS"
     gene            1696..2871
                     /locus_tag="VLC50_03380"
     CDS             1696..2871
                     /locus_tag="VLC50_03380"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014743192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HSF26539.1"
                     /translation="MARDAVIVAAVRTPTGKRGGGLAGVHPVDLSATVLDALVARTGL
                     DPALVDDVIWGCVSQTGEQAVNVARNAVLAAGWPETVPGVTLDRQCGSSQQALHFAAA
                     GVISGQYDVAVAGGVELMSRVPMLCTVTQGPGVPFGPRMDARYDGFVFDQGQGAEKIA
                     ARWGLSRADVDRIALRSHELAAAAADSGAFDAEITPVRTAAGEVRADEGIRRGGSMAS
                     LAELRTVFRADGVVTAGNASQISDGAAALLVTSSERAAELGWTPLARVVATAVVGVDP
                     VTMLTGPIPATAAVLKRAGLAVSDIGAFEVNEAFASVIGAWWAEVEKAGDLDRGELEA
                     RTNPLGGAIALGHPLGASGARLATTLVHHLQRTGARHGLQVMCEGGGMANATILERL"
     gene            complement(3023..3268)
                     /locus_tag="VLC50_03385"
     CDS             complement(3023..3268)
                     /locus_tag="VLC50_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007824467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CrgA"
                     /protein_id="HSF26540.1"
                     /translation="MPKSRGRKKTDYTPPSKAAVKVGSPVWLVPLMVGLLLLGLAWII
                     VYYVSSARFPIPGVEHWNLLIGFGLILAGFVMSTKWK"
     gene            3424..4149
                     /locus_tag="VLC50_03390"
     CDS             3424..4149
                     /locus_tag="VLC50_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019874464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HSF26541.1"
                     /translation="MRGWRLLTPAIFAAAGILFVTSAVVARGTDLRTGERTSVADVIA
                     REQANVADLGAHAEDLRAEIDKMTASIERRDGRLAEARAKIDTVAPAAGMRGVKGPAV
                     TVLLDDAPTPPAGEPIPEGLNVDSYVVHQQDVQAVVNALWAGGAEAMMLMDQRVISTS
                     AVRCVGSLLILQGRTYPPPYKITAIGDQDALLAALDASRAVSNYRDYVPLIGLGYKVE
                     VEPSVTMPGFAGALNLSHARVAS"
     gene            4146..>4636
                     /locus_tag="VLC50_03395"
     CDS             4146..>4636
                     /locus_tag="VLC50_03395"
                     /inference="COORDINATES: protein motif:HMM:NF033747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class E sortase"
                     /protein_id="HSF26542.1"
                     /translation="MSARAGAAARMAQAVTRGVGELLITAGVVILLFCAYQLFWTNVT
                     AGHATDQVVSALRQQWTQPPAPAPTGPSAAGPTTAPVPPSVPLGDALALMHIPRLGAD
                     WVKPVVQGVDLPDLARGLGHYPKTAGPGEVGNFAVAGHRATNGEPLRDIDKIKPGDTI
                     VVET"
BASE COUNT          642 a         1670 c         1676 g          648 t
ORIGIN      
        1 acccggcggc gtcgaggccc taaaggggga cgacgacaaa cccaacgggg tacgacgaaa
       61 tgcggtacga cgacctcgac cggagctggc atgcgtcgcc gtaccgatca cgaccgcgtg
      121 cgaggatggg cgccacagca ccgcagcgac cccgcgcgat ccccccgcgc gccaacagga
      181 gaggaccgtc gtggccgagc agctcttcgc gaccctgcac acgagcaagg gcgatgtcac
      241 cgtggagctc ttcccggact tcgcgcccaa gaccgtgcgc aacttcgtcg agctggccga
      301 gggcacgcgg gagtggaccc accccaagac cggggccaag accaccgaca agctctacga
      361 cggcacggtg ttccaccggg tcatccccgg cttcatgatc cagggcggtg acccgctggg
      421 caccggcacc ggcggtcccg gctacgagtt cgatgacgag ccgctcccgg agaagacctt
      481 cgaccggccc tacctgctgg ccatggccaa cgccggcaag cgcggcggca agggcaccaa
      541 cggctgccag ttcttcatca cggtggcgcc gaccacctgg ctcaacttca agcacacgat
      601 cttcggcgag gtcgccgacg gtgacggccg cgcggtcgtc gacgcgatcg cggccgtgcc
      661 gaccggggcg atggaccgcc cggtcgagcc ggtcgtcatc aagtcggtga cgatcgagcg
      721 gcgcgcgagc tgagccccgc gggactggga tgaccgacga cccgggcacc gcctcgcccc
      781 cggcggcggt gccgacctgc taccggcacc ccggccgcga gacctacatc cggtgcgccc
      841 ggtgcaaccg gccgatctgc cccgactgca tggtgccggc ctcggtcggc ttccagtgcc
      901 cggaatgcgt gcgcgagggc aacagcaccc agcgcgcgcc gcgtaccgcc tttggcggcc
      961 ggctggcgtc gggggcgcgg gtcacgatgg tgctcatcgg gctcaacgtc ctcatgtacg
     1021 tcgccgagct ggcgctgggc cgggccttcg tcaacgacct ggcgctggtc gggctggccc
     1081 gggagggctt tctcggcccg atcgtcgggg tcgccgacgg cgagtggtgg cggctggtca
     1141 ccgcgacgtt cctgcacgac cccggctcgt tcctgcacat cttgttcaac atgtacgcgc
     1201 tctacgtgct cggcccgccg ctcgaggcaa tgctcgggca cgcccgcttc ctcgcgctct
     1261 acctgctctc ggcgctgggc ggcaccgcgg cctcctacac gttctcggcg cccaactcgt
     1321 cgtcggtggg cgcgtccggg gcgatcttcg ggctgttcgc cgcgtggatc gtgctggccc
     1381 gcaagcagcg cctcgacgcg cggccgatgc tggtgctgct cggcatcaac ctggtgctcg
     1441 gcttcgtcat cgccggcatc gactggcgcg ggcacctcgg cggcatggtc accggggcgg
     1501 cggtcgcggc gatcatggcc tatgccccgc gcggaccgcg tcgtacgacg tggcaggtgg
     1561 ccggcgccgt ggcggtgctc gcgctcgtcg tgctcgtcga cgtctggcgc accgcgtccc
     1621 tgctcagctg agtcacccat tgggcgtcgt acgctgggag ccgagtgcta ctcgtcggta
     1681 acccgggagg tcggcatggc gcgtgacgcg gtgatcgtgg cggcggtgcg cacgccgacc
     1741 ggcaagcggg gcggcgggct ggccggcgtg cacccggtcg acctgtccgc gaccgtgctc
     1801 gacgcgctgg tcgcccggac cgggctcgac ccggcgctgg tcgacgacgt gatctggggc
     1861 tgcgtctcgc agactggcga gcaggcggtc aacgttgcgc gcaacgccgt actcgccgcc
     1921 ggctggccgg agacggtgcc cggcgtgacc ctcgaccggc agtgcggctc gagccagcag
     1981 gcgctgcact tcgccgccgc cggggtgatc tccgggcagt acgacgtcgc ggtcgccggc
     2041 ggggtcgagt tgatgtcgcg ggtgccgatg ctgtgcacgg tcacccaggg cccgggggtg
     2101 ccgttcggac cccggatgga cgcccgctac gacgggttcg tcttcgacca gggccagggc
     2161 gccgagaaga tcgcggcccg gtggggcctc tcccgcgcgg acgtcgaccg gatcgcgctg
     2221 cgctcgcacg agctggccgc ggcggccgcc gactccggcg cgttcgacgc cgagatcacc
     2281 ccggtgcgta cggcggcggg cgaggtgcgc gccgacgagg ggatccgccg cggcgggtcg
     2341 atggcctcgc tggccgagct gcgcacggtg tttcgcgccg acggcgtggt caccgcgggc
     2401 aacgcctcgc agatctccga tggcgccgcc gcgctgttgg tcacctcgag cgaacgggcc
     2461 gccgagctgg gctggacacc gctggcccgg gtggtggcca ccgccgtggt cggggtggac
     2521 ccggtcacca tgctcaccgg gccgatcccc gcgaccgccg cggtgctgaa gcgggccggg
     2581 ctggcggtct cggacatcgg cgcgttcgag gtcaacgagg cgttcgccag cgtgatcggc
     2641 gcctggtggg ccgaggtgga gaaggccggc gacctcgacc ggggcgagct cgaggcgcgg
     2701 accaacccgc tgggcggcgc gatcgcgctg gggcacccgc tgggtgcctc gggggcccgg
     2761 ctggccacca cgctggtgca ccacctgcag cgcaccgggg cgcgccatgg cctgcaggtc
     2821 atgtgcgagg gcggcggcat ggccaacgcg acgatcctcg agcgcctcta acccttgttg
     2881 gtgatcaagc aatcggcgta cacccgcgga cgcctgtccg tttcgtgtgc acggattgct
     2941 tgatcaccgt ggggtggcgg gggagttatc cccactgtgg atggcgggtg tggaggaatc
     3001 acagccgtgt gattaggcgg tgctacttcc acttggtgga catcacgaac ccggccagga
     3061 tcagcccgaa gccgatcagc aggttccagt gctcgacgcc agggatcggg aaccgggcgc
     3121 tgctcacgta gtagacgatg atccacgcca gcccgagcag cagcagaccg accatcagcg
     3181 ggaccagcca gaccgggctg ccgaccttca ccgcggcctt ggagggcggc gtgtagtcgg
     3241 tcttcttccg gccacgcgac ttgggcacga ctcgctcctt gtgatcaatg gtcgttgacg
     3301 gctggacacc caacgccgag ccgtcaggag gccagggccg tcagttagcg tagccacatc
     3361 atccgtccga cccgtaggac gcgggtcggc atggcgaggc cacggcgagg aggacacgac
     3421 accgtgcgcg gctggcggct gctcaccccg gcgatcttcg ccgcggccgg catcctgttt
     3481 gtcacgagcg ccgtcgtggc ccgcggcacc gacctgcgca ccggcgagcg cacctcggtg
     3541 gccgacgtca tcgcccgtga gcaggccaac gtggccgacc tgggcgcaca cgccgaggac
     3601 ctgcgcgccg agatcgacaa gatgaccgcc tcgatcgaac gacgggacgg ccggctggcc
     3661 gaggcccgcg ccaagatcga cacagtcgcg ccggccgccg ggatgcgcgg cgtcaagggc
     3721 ccggcggtca ccgtgctgct cgacgacgcg ccgaccccgc cggccggcga gccgatcccc
     3781 gagggcctca acgtcgacag ctacgtcgtg caccagcagg acgtccaggc ggtcgtcaac
     3841 gcgctgtggg ccggtggcgc cgaggcgatg atgctcatgg accagcgggt gatctcgacc
     3901 agcgccgtac gctgcgtcgg cagcctgctc atcctgcagg ggcgcaccta cccgccgccc
     3961 tacaagatca ccgcgatcgg ggaccaggac gcgctgctgg ccgccctcga cgcctcgcgg
     4021 gcggtgtcca actaccgcga ctacgtgccg ctgatcgggc tgggctacaa ggtcgaggtc
     4081 gagccgtcgg tgaccatgcc cgggttcgcc ggcgcgctca acctcagcca cgcccgggtc
     4141 gcttcgtgag cgcccgcgcc ggcgccgcgg cgcgaatggc tcaggcggtc acccgcgggg
     4201 tcggcgagct gttgatcacg gccggcgtgg tgatcctgct gttctgcgcc taccagctgt
     4261 tctggaccaa cgtcacggcc gggcatgcga ccgaccaggt ggtcagcgcg ctgcgccagc
     4321 agtggaccca gccgcccgcc ccggccccga ccggcccgtc ggcggccggc ccgaccaccg
     4381 cccctgtccc gccgagcgtc ccgctcggcg acgcgctggc gctcatgcac atcccccggc
     4441 tcggcgccga ctgggtcaag ccggtcgtgc agggtgtcga cctgcccgac ctggcccgcg
     4501 gcctgggcca ctaccccaag accgccggcc ccggcgaggt gggcaacttc gcggtggccg
     4561 ggcaccgggc caccaacggc gagccgctgc gcgacatcga caagatcaag cccggtgaca
     4621 cgatcgtggt ggagac
//
LOCUS       DATIBB010000093        11159 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_179634, whole genome shotgun sequence.
ACCESSION   DATIBB010000093 DATIBB010000000
VERSION     DATIBB010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 11159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11159
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179634"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..357
                     /locus_tag="VLC50_03400"
     CDS             <1..357
                     /locus_tag="VLC50_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010224853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 family protein"
                     /protein_id="HSF26543.1"
                     /translation="EVLAAGRRTVLELVDDPTSELRVRSRDLLASWGAHLTVDTRAAA
                     KVDGWLAQAAVYVVEGYREEITRLITDTVDRWDGRETARRIELQVGRDLQFIRLNGTV
                     VGSLVGVLIHAFSQLL"
     gene            complement(389..1564)
                     /locus_tag="VLC50_03405"
     CDS             complement(389..1564)
                     /locus_tag="VLC50_03405"
                     /inference="COORDINATES: protein motif:HMM:NF017569.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HSF26544.1"
                     /translation="MPSGRDLATLAGAFGDLLRRSGVPVTPERAARFAAATTLARPAD
                     VGELYWLARVTLLDGPGQIEVFERVFNQVFRGIADVADFRGDAAAPPPPPEVKPGTNR
                     RASAHPATVESDAPMPALTGAPTGPEGSANPDTVLAAASAEERLRTKDFASCSPEELA
                     LVARLVAALRLSPPRRPARRTVSRPSGRRLDVRRTLRRAHRTGGDPVRREFRRRAERT
                     RRVVLIADVSGSMEAYSRVYLHLLAGAVRGARAEAFVFATRLTRLTRALRTGPPEVAL
                     ARAMHEAPDWSGGTRIGSALKAFLDGHGRRGLARGAVVVIVSDGWERADPAQVGEQMA
                     RLARLAHRVVWVNPRKAAEGFAPLTGGMAAALPHVDAFVSGHSAAALDDVLAAIAST"
     gene            complement(1567..2436)
                     /locus_tag="VLC50_03410"
     CDS             complement(1567..2436)
                     /locus_tag="VLC50_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017981210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HSF26545.1"
                     /translation="MLPDVTQVADALAAVDYLADPGLATALFCAARLPQPILLEGEAG
                     VGKTEAAKALAAVLDTPLIRLQCYDGIDAAEALYEWNYPRQLLGIRLAEARGGQLGEG
                     DLFGRDYLVARPLLAAIEHPGPRPAVLLIDEVDRADEEFEAFLFELLAESAITIPEIG
                     TIRARIAPLVVLTSNRTRDLHDALKRRCLYHWIDFPDLERTVAIIRRRVPAAAVDLTT
                     QVTNAVGRLRSLDLQKPPGVAEAIDWVHALSLLGVRTLNAEAARRTLGSVLKYREDHD
                     VADGRGLDWVVGG"
     gene            complement(2463..3422)
                     /locus_tag="VLC50_03415"
     CDS             complement(2463..3422)
                     /locus_tag="VLC50_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006237882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HSF26546.1"
                     /translation="MTRASVRARTAELRAQRVPFVHAQVVMAQRPTSAKPGDEAIIYA
                     DGTIEGFVGGSCAESTVRAQSLALLDSGDPLLLRITPDVDEAADAAPAPATSAAEGRG
                     LVSVHNPCLSGGTLEIFLEPMVPAPLLAVHGQSPIAAALVALGPHLGWEVVDWDETDD
                     TARAAVAIVVAAHGRDEEDVLTAALAAGTPYVGLVASPTRGAAVLASLDVDETAKARV
                     RTPAGLDIGARTPEEVALSILAEVVAARRHPSGRPIADAAAEPAPAPPPSPAVAIDPV
                     CGMSVAAVDASLHLDHDGTRWWFCGTGCLRAFAADPSAYLQTS"
     gene            complement(3473..5878)
                     /locus_tag="VLC50_03420"
     CDS             complement(3473..5878)
                     /locus_tag="VLC50_03420"
                     /EC_number="1.2.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015750357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic carbon-monoxide dehydrogenase large
                     subunit"
                     /protein_id="HSF26547.1"
                     /translation="MTTTAETPAAVATSPAGNPMGFGRMLRKEDARFLRGQGHYVDDI
                     ALPGMLHGAILRSPFAHARIVSIDTSAAEAHPKVKAVITGATLEGLNLAWMPTLSMDV
                     QAVLATDKVRFQGQEVAFVIAEDKYSARDALELIDVDYEPLPPVVDARKALDPGAPVI
                     RDDIEGKTDNHIFDWESGDAAATDEVFANAEVVVSQEMLYPRSHPSPMETCGTVASMD
                     RVTGKLTVWSTNQAPHAHRTVYALVAGIPEHKIQIISPDIGGGFGNKVGIYPGYVLAV
                     VGSIVTGKPVKWVEDRSENLMSTAFARDYHMKGQIAATRDGKILGVRVDVLADHGAFN
                     GTAQPTKFPAGFFHIFTGSYDLAAAHCKVTGVYTNKAPGGVAYACSFRITEAVYLVER
                     MVDVLAAELGVDPGELRMKNLLRPEQFPYTTKTGWEYDSGNYPRALRKAMDMVGYEDL
                     RREQAEKRARGVYMGIGISFFTEGVGAGPRKHMDILGLGMADGCELRVHPTGKAQLRL
                     SVQTQGQGHETTFAQIVAHELGLPPEDVEVLHGDTDNTPFGLGTYGSRSTPVSGAAAA
                     VVSRRVRDKARLVAAAALEVSPDDLEWELGRWFVKGDPSQGKTIQEIAMLSHGNLELP
                     EGVEGHLDASVVYDPPNLTYPFGAYIAVVDLDPGTGQVKVRRFVAVDDCGPRINPMIV
                     EGQIHGGLADGVGMALMQVIAFDEDGNNLGGSFMDYLLPTSIECPSWETGETVTPSPH
                     HPLGAKGVGESATVGSPPAVVNAVIDAMAPFGVRHADMPLTPANVWRSIQGNPFRTDM
                     AVS"
     gene            complement(5875..6390)
                     /locus_tag="VLC50_03425"
     CDS             complement(5875..6390)
                     /locus_tag="VLC50_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020104850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HSF26548.1"
                     /translation="MQVSMTVNGSPVTAEIEPRLLLIHFVRDHLRLTGTHWGCDTTQC
                     GTCVLWLDGQPVKSCTVLAAMADGHEVRTVEDLERDGELDVVQQGFMNEHGLQCGFCT
                     PGMMMTARWLLDHDPDPDETTIREAISGQICRCTGYENIVRAVRWAAVHGPDGSVVST
                     EPVDVPEGASA"
     gene            complement(6392..7264)
                     /locus_tag="VLC50_03430"
     CDS             complement(6392..7264)
                     /locus_tag="VLC50_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015750355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HSF26549.1"
                     /translation="MQTPAPVDYERATSVDDALARLARLGPESRLIAGGHSLLPMMKL
                     RLARPETVIDINGLSDLDYIRVEGSELVLGALTRHAALLASPVVGEHYAMIPDAERVI
                     ADPIVRNRGTVGGSFCQADPAEDLSAVGSALKATLVIRSAQGTRTVPAREFHEGPYET
                     VVGDAEILIEVRIPIRPGAGSAYEKVERRAGDWAAAAAGAYIVLADGVISDVGIGLTA
                     VGAPHFCAPEAEAYLVGKPPTDEHRAEAGRRAAEASSPSADQRGPVDYKRHLAGELTV
                     RALRRSVARAEGRV"
     gene            7470..8480
                     /locus_tag="VLC50_03435"
     CDS             7470..8480
                     /locus_tag="VLC50_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003991769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HSF26550.1"
                     /translation="MERVVKRSVVNWTSGAADIETYLSAQVSDLSHREVVGPLKAAAG
                     ASGVVLVGGSEVAAWGDPDEPEMAFSVTKSVVSVVAGLAYDDGLLVPDQPVHEVLDVE
                     QFRGPHNERITWRHLLHQSSQWEGELWGKPTRVDAQSYREGTEVHGTPPGEGWAYNDV
                     RMNLLTYALTLLFRQSLAEVLRSRVMEPLAASDHWSWHGYRTSKVELDGTSVEVVSGG
                     AHWGGGLFIPARDLALVGQLFLDHGMHGGVRVLSAEWVEQSWAPCPIKPEYGYLWWLN
                     DDGVPWPGAPSTGRSARGNAGRHLLWVDPARDLVLASHWTEEPLALITDVSSAASPRP
                     WN"
     gene            complement(8502..11012)
                     /locus_tag="VLC50_03440"
     CDS             complement(8502..11012)
                     /locus_tag="VLC50_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016699662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3516 domain-containing protein"
                     /protein_id="HSF26551.1"
                     /translation="MVELLPSSPDPDALFDAFAGWASEAGFELYPHQQDALIELVSGS
                     NVILSTPTGSGKSLVAAGAHFAALATGRRTFYTAPIKALVSEKFFALIDLYGAERVGM
                     LTGDASVNPGAPIICATAEVLANLALREGARADVGQVVMDEFHFYAEPDRGWAWQVPI
                     LELPHAQFLLMSATLGDVSFFEADLTRRTGRPTAVVASAERPVPLTFRYATTPLHETI
                     AELLEGLQAPVYVVHFTQAAALERAQALMSVNVCTRAEKDAIAELLGGFRFGAGFGKT
                     LSRLVRHGIGVHHAGMLPRYRRLVEQLAQAGLLKVICGTDTLGVGINVPIRTVLLTSL
                     SKYDGRRTRLLKAREFHQIAGRAGRAGYDTVGYVVVEAPEHDIENARALAKAGDDPKK
                     RRKVVRKKPPEGSVSWSQATFDKLVTAAPEPLRSRFRVSHSMVLNVIARPGDAFTAMR
                     HLLEDNHDDRPRQRQHIRRAIGIYRALLTAGVVERLDEPDAAGRTVRLTVDLQADFAL
                     NQPLSPFAFAALGLLDRESPTYALDVLSVIEATLDDPRQVLLAQQHKARGEAVAQMKA
                     DGIEYDERMELLDEVSWPKPLAELLEGAFETYRRGQPWVADYELSPKSVARDMYERAL
                     TFTELVGFYGLSRSEGQVLRYLADAFRALRQTVPAEAKTDELVDIVEWLGETVRQTDS
                     SLLDEWEALRNPTLVLTAEPTVDDNTPPPVTANARAFHVLVRNAMFRRVELAARRRYD
                     ELGELDADSGWDADAWRAALDGYFAEHDEIGTGADARGPKMLLVDKAADASRRWLVRQ
                     IFDDPSGDHDWGVSAEVDLDASDEAGAAVVRVTAVDRL"
BASE COUNT         1504 a         3830 c         4217 g         1608 t
ORIGIN      
        1 gaggtgctgg ccgcgggccg tcgtaccgtc ctggagctcg tcgacgaccc gacgagtgag
       61 ctgcgggtgc gcagccgtga cctgctggcc tcctggggag cccacctgac cgtggacacc
      121 cgggccgcgg ccaaggtcga cggctggctg gcgcaggcgg ccgtctacgt cgtcgagggc
      181 taccgcgagg agatcacccg gctgatcacc gacaccgtcg accgctggga cgggcgggag
      241 accgcgcgcc ggatcgagct gcaggtcggg cgtgacctgc agttcatccg gctcaacggc
      301 acggtcgtcg ggtcgctggt cggcgtgctg atccacgcct tctctcagct gttgtgaggg
      361 catcggcgcc cggccgcggc gcgtggtgtc acgtcgaggc gatggcggcg agcacgtcgt
      421 cgagcgcggc ggccgagtgc cccgacacga aggcgtcgac gtgcggcagc gccgcggcca
      481 tcccgccggt caacggggcg aacccctcgg cggccttgcg cgggttgacc cagaccaccc
      541 ggtgagccag ccgggccagg cgggccatct gctcgcccac ctgggccggg tcggcgcgct
      601 cccacccgtc ggagacgatc accacgacgg cgccccgggc caagccccga cggccgtgcc
      661 cgtcgaggaa cgccttcagc gcggagccga tgcgggtgcc ccccgaccag tcgggcgcct
      721 cgtgcatcgc ccgggccagg gccacctcgg gtgggccggt gcgcagcgct cgggtcagcc
      781 gggtcagccg ggtcgcgaac acgaacgcct cggcccgggc gccgcggacc gcgcccgcca
      841 gcaggtgcag gtagacccgc gagtaggcct ccatcgaccc ggacacgtcg gcgatcagca
      901 ccacccggcg ggtccgctcg gcgcggcggc ggaactcgcg ccgcaccggg tcacctccgg
      961 tgcggtgggc tcggcgcagc gtccgtcgta cgtcgagccg ccgtccggac ggtcgggaga
     1021 ccgtacgacg tgccggccgg cggggcgggc tcagccgcag cgccgcgacc agccgcgcca
     1081 ccagggccag ctcctccggg ctgcacgacg cgaagtcctt ggtccgcagc cgttcctccg
     1141 cgctggccgc ggcgagcacc gtgtcggggt tggcgctgcc ctccgggccg gtcggtgcgc
     1201 cggtcagggc cggcatcggg gcgtcggact cgacggtggc cgggtgggcg gaggcgcgcc
     1261 ggttggtgcc cggcttcacc tcgggcggcg gcggcggtgc ggcggcgtcg ccgcggaagt
     1321 cggccacgtc ggcgatgccg cggaacacct ggttgaacac ccgctcgaac acctcgatct
     1381 ggccgggccc gtcgagcagg gtgacccggg cgagccagta gagctcgccg acgtcggccg
     1441 gccgggccag cgtggtcgcg gcggcgaacc gggcggctcg ctccggcgtg accggcacgc
     1501 cggagcggcg caacaggtcg ccgaacgcgc ccgccagcgt ggccaggtca cggccggacg
     1561 gcacgctcag ccgccgacga cccagtcgag gccacggccg tcggcgacgt cgtggtcctc
     1621 gcggtacttc agcaccgagc ccagcgtgcg ccgggctgcc tcggcgttga gcgtgcgcac
     1681 cccgagcagg ctcagcgcgt gcacccagtc gatcgcctcg gccaccccag ggggtttctg
     1741 caggtcgagc gagcgcagcc gcccgacggc attggtcacc tgcgtggtga ggtcgaccgc
     1801 ggcggccggg acacggcgcc ggatgatcgc gaccgtgcgc tcgaggtcgg ggaagtcgat
     1861 ccagtggtag aggcagcgcc gcttgagcgc gtcgtgcagg tcgcgggtgc ggttggaggt
     1921 gagcacgacc agcggggcga tcctggcccg gatggtgccg atctcgggga tcgtgatcgc
     1981 cgactcggcg agcagctcga acaggaacgc ctcgaactcc tcgtcggccc ggtccacctc
     2041 gtcgatgagc agcaccgcgg ggcgcgggcc ggggtgctcg atggccgcga gcagcgggcg
     2101 ggccacgagg tagtcgcgcc cgaacaggtc gccctcgccg agctgcccgc ctcgcgcctc
     2161 ggcaagtcgg atgcccagca gctggcgggg gtagttccac tcgtagagcg cctctgccgc
     2221 gtcgatgccg tcgtagcact gcagccggat cagcggggtg tcgagcaccg ccgccagcgc
     2281 cttggccgcc tcggtcttgc cgacgccggc ctccccctcg agcaggatcg gctgcggcag
     2341 acgggcggcg cagaacagcg cggtcgccag gcccgggtcg gccaggtagt cgacggcggc
     2401 cagggcgtcg gcgacctggg tgacgtcggg caacagctcg tcgacggggg tcgaggcggc
     2461 gttcacgacg tctgcaggta ggccgacggg tcggcggcga acgcgcgcag acagcccgtg
     2521 ccgcagaacc accagcgggt cccgtcgtgg tcgaggtgca gcgacgcgtc gaccgcggcg
     2581 accgacatcc cgcacacggg gtcgatcgcg accgcggggc tgggcggggg agccggggcc
     2641 ggctcggccg ccgcgtcggc gatcgggcgc ccgctcgggt gccgccgggc cgcgacgacc
     2701 tcggcgagga tcgacagcgc cacctcctcg ggggtgcgcg ccccgatgtc gagaccggcg
     2761 ggcgtgcgga cccgggcctt ggccgtctcg tccacgtcga ggctggccag cacggcggca
     2821 ccgcgcgtcg ggctggcgac cagcccgacg tagggcgtgc cggccgccag cgcggcggtc
     2881 agcacgtcct cctcgtcgcg gccgtgcgcg gccaccacga tggccaccgc ggcccgcgcg
     2941 gtgtcgtcgg tctcgtccca gtcgacgacc tcccagccca ggtgcggccc gagcgcgacc
     3001 agggcggccg cgatcgggct ctgcccgtgt acggcgagca gcggggccgg caccatgggc
     3061 tcgaggaaga tctccaacgt ccctccggac aggcacgggt tgtgcaccga caccaggccg
     3121 cgcccctcgg cggcggaggt ggccggcgcc ggggccgcgt cggccgcctc gtcgacgtcc
     3181 ggggtgatcc gcaggagcaa cgggtcgccg gagtcgagca gggccaggct ctgcgcccgc
     3241 acggtcgact cggcgcacga gccgccgacg aacccctcga tcgtgccgtc ggcatagatg
     3301 atcgcctcgt cgccgggctt ggccgaggtc ggacgttggg ccatcaccac ctgggcgtgg
     3361 acgaacggca cccgctgggc gcgcagctcc gccgtccgtg cccgcaccga ggcgcgggtc
     3421 acggacgaac ggccaaccaa gcgatcggcc gagctctgcg agggctcggg tgtcacgaca
     3481 ccgccatgtc ggtccggaac gggttgccct ggatggaccg ccagacgttg gccggggtca
     3541 gcggcatgtc ggcgtgacgg accccgaagg gagccatcgc gtcgatgacg gcgttgacca
     3601 ccgcgggcgg tgacccgacg gtggccgact cgccgacgcc cttggcgccc agcgggtggt
     3661 gcggcgacgg cgtcaccgtc tcgccggtct cccagctggg gcactcgatc gaggtgggca
     3721 gcaggtagtc catgaacgac ccgccgaggt tgttgccgtc ctcgtcgaag gcaatgacct
     3781 gcatcagcgc catgccgacc ccgtcggcca gcccgccgtg gatctggccc tcgacgatca
     3841 tcgggttgat ccgcggcccg cagtcgtcga cggcgacgaa gcggcgcacc ttgacctgcc
     3901 cggtgcccgg gtcgaggtcg accacggcga tgtaggcgcc gaacgggtag gtgaggttgg
     3961 gcgggtcgta gacgaccgag gcgtcgaggt gaccctcgac cccctcgggc agctcgaggt
     4021 tgccgtggct gagcatggcg atctcctgga tcgtcttgcc ctggctcggg tcgcccttga
     4081 cgaaccagcg gcccagctcc cactcgaggt cgtcagggct gacctcgagc gcggcggccg
     4141 cgacgaggcg agccttgtcg cggacccggc gcgagactac ggccgccgcg gccccggaga
     4201 ccggggtgga ccgggaccca taggtgccca gcccgaacgg cgtgttgtcg gtgtcgccgt
     4261 gcagcacctc gacgtcctcg ggaggcaggc cgagctcgtg ggcgacgatc tgggcgaacg
     4321 tcgtctcgtg gccctggccc tgggtctgca ccgacaggcg cagctgggcc ttgccggtcg
     4381 ggtgcacccg cagctcgcag ccgtcggcca tgcccaagcc caggatgtcc atgtgcttgc
     4441 gcggcccggc gcccacgccc tcggtgaaga agctgatccc gatgcccatg taaacgcccc
     4501 gcgcgcgctt ctcggcctgc tcacggcgca ggtcctcgta gccgaccatg tccatggcct
     4561 tgcgcagcgc ccgcgggtag ttgccggagt cgtactccca gccggtcttg gtggtgtagg
     4621 ggaactgctc ggggcgcagg aggttcttca tccgcagctc gcctgggtcg acgccgagct
     4681 cggcggccag gacgtcgacc atccgctcga ccaggtagac cgcctcggtg atccggaacg
     4741 agcaggcgta ggccacgccg ccgggggcct tgttggtgta gacgccggtg accttgcagt
     4801 gggcggccgc caggtcatag ctgccggtga agatgtggaa gaacccggcc gggaacttcg
     4861 tcggctgcgc ggtgccgttg aacgccccgt ggtcggcgag cacgtcgacc cgcacgccga
     4921 ggatcttgcc gtcgcgggtc gccgcgatct ggcccttcat gtggtagtcg cgggcgaacg
     4981 ccgtggacat gaggttttcg ctgcggtcct ccacccactt gaccggcttg ccggtcacga
     5041 tcgagccgac cacggccagc acgtagccgg ggtagatgcc gaccttgttg ccgaagccgc
     5101 cgccgatgtc gggggagatg atctggatct tgtgctcggg gatgccggcg accagcgcgt
     5161 agacggtgcg gtgggcgtgc ggggcctggt tggtcgacca gaccgtgagc ttgccggtga
     5221 cccggtccat gctcgccacc gtgccgcagg tctccatcgg cgaggggtgc gagcgcgggt
     5281 agagcatctc ctggctgacc acgacctcgg cgttggcgaa cacctcgtcg gtggccgccg
     5341 cgtcgcctga ctcccagtcg aagatgtggt tgtcggtctt gccctcgata tcgtcgcgga
     5401 tcaccggggc ccccgggtcg agggccttgc gggcgtcgac gaccggcggc agcggctcgt
     5461 agtcgacgtc gatgagctcg agcgcgtcgc gggcggagta tttgtcctcg gcgatgacga
     5521 aggcgacctc ctggccctgg aagcgcacct tgtcggtggc cagcaccgcc tgcacgtcca
     5581 tcgacagcgt cggcatccac gccaggttga ggccctcgag cgtggcgccg gtgatgacgg
     5641 ccttgacctt ggggtgcgcc tcagcggccg aggtgtcgat cgagacgatg cgggcgtggg
     5701 cgaacgggct gcgcaggatc gcgccgtgga gcatgcccgg cagcgcgatg tcgtcgacgt
     5761 agtggccctg cccgcgcagg aaccgggcgt cctccttgcg cagcatccgg ccgaacccca
     5821 tggggttgcc ggccgggctg gtggcgacgg cggcgggagt ctctgccgtg gtggtcatgc
     5881 cgatgctcct tccggtacgt cgacgggttc ggtggagacc acggacccat cgggaccgtg
     5941 cacggcggcc cagcgcacgg cccgcacgat gttctcgtag ccggtgcacc ggcagatctg
     6001 cccggagatg gcctcgcgga tcgtcgtctc gtcggggtcg gggtcgtggt cgagcagcca
     6061 gcgggcggtc atcatcattc ccggggtgca gaacccgcac tgcagcccgt gctcgttcat
     6121 gaacccttgc tgtacgacgt cgagctcgcc gtcgcgctcg aggtcttcga cggtgcgcac
     6181 ctcgtggccg tcggccatcg cggcgagcac ggtgcagctc ttgaccggct ggccgtccag
     6241 ccacagcacg caggtgccgc actgggtggt gtcacagccc cagtgggtgc cggtcagcct
     6301 gaggtggtcg cgcacgaagt ggatcagcag cagccgcggc tcgatctcgg ccgtgaccgg
     6361 cgagccgttg acagtcatcg acacctgcat gtcagaccct tccctcggcg cgggccacgg
     6421 agcggcgcag cgcacggacc gtgagctcgc cggccaggtg gcgcttgtag tcgaccgggc
     6481 cgcgctggtc ggcgctgggc gaggaggcct cggcggcccg ccggccggcc tcggcccggt
     6541 gctcgtcggt gggcggcttg ccgacgagat aggcctcggc ctcgggagcg cagaagtgcg
     6601 gggcgccgac cgcggtcagc ccgatgccga cgtcgctgat cacgccgtcg gcgagcacga
     6661 tgtaggcgcc ggccgctgcg gcggcccagt caccggcccg ccgctcgacc ttctcgtagg
     6721 cgctgccggc cccggggcgg atcgggatcc gcacctcgat gaggatctcg gcgtcgccca
     6781 ccacggtttc gtaggggcct tcgtggaact cgcgggcggg cacggtgcgg gtgccctgcg
     6841 cggagcggat cacgagggtg gccttgagcg cggagccgac ggccgacagg tcctcggccg
     6901 ggtcggcctg gcagaacgag ccacccacgg tgccgcggtt gcgcacgatc gggtcggcga
     6961 tgacccgctc ggcgtcaggg atcatcgcgt agtgctcgcc gacgaccggt gaggccagca
     7021 gtgcggcgtg tcgggtcagt gccccgagca ccagctcgga gccctcgacg cggatgtagt
     7081 cgaggtcgct caacccgttg atgtcgatga ccgtctcggg gcgggccagc cgcagcttca
     7141 tcatcggcag caggctgtgc ccgcctgcga tgagccggga ctccgggccg agccgggcga
     7201 gccgggccag ggcgtcgtcg acgctcgtgg ccctttcgta gtcaactggt gccggagttt
     7261 gcatccgttg aaccccctat gcgtggaatg acacgccaga tcggccgctg ggcacctgat
     7321 ggccgcggcc gtgccgacca ctctgccgcg ctttgctgcc aaagacaacg atgcccacga
     7381 caacggagtg gccggcccgc cggttcggcc tggagcgccg gagcggcaga ctcgacggcg
     7441 tggacaggag tcgcatcgat caggcgggca tggagcgtgt cgtcaagcga agcgtcgtca
     7501 actggacgag cggcgccgcc gacatcgaga cctatctctc ggctcaggtg tcggacctca
     7561 gccaccggga ggtcgtcggt ccgctcaagg ccgctgccgg cgccagcggt gtcgttctcg
     7621 tcggcgggtc tgaggttgcc gcgtggggtg accccgacga gccggaaatg gctttcagcg
     7681 tcacaaaatc ggtcgtgtct gtggttgctg ggctcgccta cgacgacggg ctcctcgtcc
     7741 cggaccaacc ggtccacgag gtgcttgatg tcgagcagtt tcgtggaccg cacaacgaga
     7801 ggatcacgtg gcggcacctg ctgcatcagt cctcacagtg ggagggggag ctgtggggca
     7861 agccaacaag ggttgacgcg cagagctacc gggaagggac ggaggtccac ggaacgcctc
     7921 ctggtgaggg ctgggcctac aacgacgtcc ggatgaacct cctcacctat gcgctcaccc
     7981 tcctgttccg gcagtctctg gccgaggtgc tgcggtcccg cgtcatggag ccgctggcgg
     8041 cgtctgacca ttggtcgtgg catgggtaca ggacgagcaa ggttgagctc gacgggacgt
     8101 cggttgaggt cgtttctggc ggtgcgcact ggggcggagg gctgttcatc cccgcccgtg
     8161 acctcgcact tgtcggacag ctcttcctgg accacggaat gcacggcggt gtccgggtgc
     8221 tcagcgcgga gtgggttgag cagtcgtggg ccccgtgccc catcaagccg gagtacggct
     8281 atctctggtg gttgaacgac gacggggtgc cctggccggg ggcgccgagc actggccgca
     8341 gcgcccgggg caacgccggt cgccacctgc tctgggtcga ccccgcacgt gacctggtcc
     8401 tcgcgtcgca ctggaccgag gagccgctcg ccctgatcac tgacgtctcg agcgctgcct
     8461 caccacgacc ctggaactga cgctcgagga tctcgaggtc ctcagagccg gtcgacggcg
     8521 gtgacgcgta cgacggccgc gcccgcctcg tcggaggcgt cgaggtcgac ctcggcgctg
     8581 acgccccagt cgtggtcgcc cgaggggtcg tcgaagatct ggcgcaccag ccaccgccgg
     8641 gacgcgtcag cggccttgtc gacgagcagc atcttgggtc cgcgggcgtc ggcgccggtg
     8701 ccgatctcgt cgtgctcggc gaagtagccg tccagcgccg cgcgccaggc gtcggcgtcc
     8761 cagcccgagt cggcgtcgag ctcgcccagc tcgtcgtagc gccggcgcgc ggccagctcg
     8821 acccggcgga acatcgcgtt gcggaccaag acgtggaagg cgcgggcgtt ggcggtcacc
     8881 ggcggcgggg tgttgtcgtc gacggtcggc tcggcggtga gcaccagggt cgggttgcgc
     8941 agcgcctccc actcgtcgag caggctcgag tcggtctgac gcaccgtctc gccgagccac
     9001 tcgacgatgt cgacgagctc gtcggtcttg gcctcggccg gcacggtctg gcgcagcgcc
     9061 cggaacgcgt cggccaggta gcgcagcacc tgtccctcgg agcgcgagag gccgtagaag
     9121 ccgacgagct cggtgaacgt cagcgcccgt tcatacatgt cgcgggccac cgacttgggc
     9181 gagagctcgt agtcggcgac ccacggctgg ccgcgccggt aggtctcgaa ggccccctcg
     9241 agcagctcgg ccagcggctt gggccagctc acctcgtcga ggagctccat gcgctcgtcg
     9301 tactcgatgc cgtctgcctt catctgggcg accgcctcgc cgcgagcctt gtgctgctgg
     9361 gccagcagca cctggcgcgg gtcgtcgagg gtggcctcga tcactgacag tacgtcgagg
     9421 gcataggtag gcgactcgcg gtcgagcagg ccgagggccg cgaacgcgaa cggcgagagc
     9481 ggctggttga gcgcgaagtc ggcctgcagg tcgaccgtca gccgcacggt gcggcccgcc
     9541 gcgtcgggct cgtcgagccg ttcgacgacc ccggcggtga gcagggcccg gtagatgccg
     9601 atggcccggc ggatgtgctg acgctgccgg ggccggtcgt cgtggttgtc ctcgagcagg
     9661 tggcgcatcg cggtgaaggc gtcgccgggg cgggcgatga cgttgagcac catcgagtgg
     9721 ctgacccgga accgagagcg cagcggctcg ggcgcggcgg tgacgagctt gtcgaaggtc
     9781 gcctgcgacc agctcaccga gccctcgggc ggcttcttgc gcaccacctt gcgccgcttc
     9841 ttggggtcgt caccggcctt ggccagcgcc cgggcgttct cgatgtcgtg ctcgggggcc
     9901 tcgacgacga cgtagccgac ggtgtcgtag ccggcccggc cggcccggcc ggcgatctgg
     9961 tggaactcgc gggccttgag cagccgggtg cgccggccgt cgtacttgct caggctggtc
    10021 agcagcacgg tgcggatcgg cacgttgatg ccgacgccga gcgtgtcggt gccgcagatc
    10081 accttgagca gcccggcctg ggccagctgc tcgaccaggc gccggtagcg cgggagcatg
    10141 ccggcgtggt gcaccccgat gccgtggcgc accagccggg acagcgtctt gccgaacccg
    10201 gcgccgaacc ggaagccgcc gagcagctcg gcgatcgcgt ccttctcggc ccgggtgcac
    10261 acgttgacgc tcatcagcgc ctgggcccgt tcgagggcgg ccgcctgggt gaagtgcacg
    10321 acgtagaccg gtgcctgcag tccctcgagc agctcggcga tcgtctcgtg cagcggcgtg
    10381 gtggcgtagc ggaacgtcag cggcaccgga cgctcggccg aggcgaccac ggcggtcggg
    10441 cgaccagtgc gccgggtcag gtcggcctcg aagaacgaga cgtcgccgag agtggccgac
    10501 atgagcagga actgcgcgtg gggcagctcg aggatcggca cctgccaggc ccagccgcgg
    10561 tcgggctcgg cgtagaagtg gaactcgtcc atcacgacct ggccgacgtc ggcgcgcgcg
    10621 ccctcgcgca gggccaggtt ggccagcacc tcggcggtgg cgcagatgat cggggcgcct
    10681 gggttgacgc tcgcgtcgcc ggtgagcatg ccgacccgct ccgcgccgta caggtcgatg
    10741 agggcgaaga acttctcgct gaccagcgcc ttgatcgggg cggtgtagaa ggtgcggcga
    10801 ccggtggcca gcgccgcgaa gtgcgcgccg gccgcgacca ggctcttgcc cgacccggtg
    10861 ggggtgctga ggatgacgtt ggagccggag accagctcga tcagggcgtc ctgctggtgc
    10921 gggtagagct caaagcccgc ctcgctggcc cagccggcga aggcgtcgaa gagcgcatca
    10981 gggtccggcg acgacggcag cagctcgacc agagtcgggg tggaaaggct cggggcggac
    11041 aggctcgcgg tggaggcgtt cgacgtcgtc gtcacgatcg atggtcccag ccgagccgtg
    11101 aggctcagcc gcgggtgcgt ggtcgaggca tcagccggct gttgggcagc tcgggggcg
//
LOCUS       DATIBB010000094         9727 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_1627810, whole genome shotgun sequence.
ACCESSION   DATIBB010000094 DATIBB010000000
VERSION     DATIBB010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 9727)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9727)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9727
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1627810"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..467
                     /locus_tag="VLC50_03445"
     CDS             <1..467
                     /locus_tag="VLC50_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012921280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HSF26552.1"
                     /translation="IAEERGMRGGHHPLLFQLLNEMDGLAEDADVVFVLTTNRADLLE
                     PALAARPGRVDQAVELGLPDAAARRALLDLYRGQLQVDPTDLDRAAAATDGVTASFLK
                     ELLRRAALVAAEDAPGAIPVLLKGTHLDDALRELQDSRNAMTRALLGSVPSV"
     gene            complement(590..1888)
                     /locus_tag="VLC50_03450"
     CDS             complement(590..1888)
                     /locus_tag="VLC50_03450"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011757879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HSF26553.1"
                     /translation="MARLLRMPAVAANATEAVLADWPVAEGTAFSATDTLATVETEKA
                     VVDVEADADGVILKTLVSPGAEVEVGAPIAILGDHGEVIDDLDALLVSLGVEAPAAAP
                     TQVNGSATPADAATPAAPAPASTAPAATPADGAVVDRLPGARIFASPLARRLAKQAGL
                     GLDQIVGTGPGGRIVRRDVDTALAGRRTAPAAPVTAPAPAASASVPGDFTDTAPSRIR
                     KVIAARLTQSKQTTPHFYLRGTAKVDALLAARAELNEVSPTKISINDMVIKAVARAHM
                     LVPAVNTIWLDDVIRSFSSVDIAVAVATDRGLVTPVLRAVERTPISEVAAQVQDFVAR
                     AKAGTLRQDELEGGAVSITNLGMYGTEEFAAIINPPQSAILAVGAAQAEPVVVDGEIR
                     AATVMRVTLSVDHRAIDGALAADWMRTFIGLLEHPVRIFA"
     gene            complement(1892..4072)
                     /locus_tag="VLC50_03455"
     CDS             complement(1892..4072)
                     /locus_tag="VLC50_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007078151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HSF26554.1"
                     /translation="MTQRNRLEPAAPWIQIVATADDWDAADPGLLTGMLSQLVLIRTF
                     EEYVLELAGAGLVHGPAHSSIGQEGGAVGSIIGLTSADGVNGSHRGHHQFLAKALTHL
                     APKGLDPTAPFPEDVRQALLTTLAEICGLARGFSSGRGGSMHLQWKEAGAMGTNAIVG
                     GGVPQAVGFAWADRHAGTDAVSVTYFGDGAANIGSTLESFNLAAAWHLPVCFFIENNQ
                     YAVSTTVFEATGEPRLSARGPGFNIPSWKVDGMDPLAVYLAMQEALAHMRAGKGPTLI
                     EADVYRYFHQNGPFPGSAFGYRSKEEEREWRARDPIEALAGHVVRRQILTQDQIDDLR
                     ARAKTLMAELGDVLLEPDPDGKPGQRRIKPSEWPDPSFRDVGVRGDLSEFAGARFADR
                     DTFTGELAEQKFIDAVAGVMGRRMATDDSIIVLGEDVHRLSGGTNGATKGLAAAYPDR
                     ILGTPISENAFTGLGCGIALDGRFKPVVEFMYADFMWVAADQLFNQIGKARHMFGGHS
                     DIPLVLRSKVAMGTGYGSQHSMDPAGVFATNPGWRIVAPSTPFDYVGLMNSALRCKDP
                     VLVLEHVDLYTSTGEGPVDDLDYCLPVGKAALRRTGSQLTVISYLAMVNYCLQALDAL
                     PDVSADLIDLRWLDRASIDWDTIGESIKKTNNVLIAEQGALGTSYGGWLADEIQRRFF
                     DWLDSPIQRVTGGEASPSISKVLERAAIAKSEEVVAKLTEITES"
     gene            4220..4747
                     /locus_tag="VLC50_03460"
     CDS             4220..4747
                     /locus_tag="VLC50_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015747383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HSF26555.1"
                     /translation="MADATGLPGLSGVDHIGFTVPDLEEATRFLIDVLGCEYMYTLGP
                     FSDSDGDWMSEHLNVDPRTVMQQLHFFRCGGQAIFEVFQYAAPDQRAQGPRNSDIGGH
                     HVALYVDDLDKAVDYLKAKGVRMLGEPTASKGPSLGQRWVYFLAPWGMQFELVSYPDG
                     KAFDKQAGDGAATSS"
     gene            4744..5400
                     /locus_tag="VLC50_03465"
     CDS             4744..5400
                     /locus_tag="VLC50_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018225458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HSF26556.1"
                     /translation="MIGSKVEAASQRVADYLRQAILTGEIGPGERIRQREVAERLGAS
                     RLPVREALRILEAEGLTEHEMNKGARVPVLDLHEVQVVYQTRERLEPLALIESMPRLT
                     ADDLAELRRIQDRIAAAPDITTFLTLDRQFHLLTYSGCSTGSLNSIVTRLWNSTQHYR
                     RAFVTISGPGRMWIVNAEHELLLDAITRADTVDAERFLSGHIRRTRIELAQHPEAFAG
                     "
     gene            5553..7235
                     /locus_tag="VLC50_03470"
     CDS             5553..7235
                     /locus_tag="VLC50_03470"
                     /inference="COORDINATES: protein motif:HMM:NF024842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HSF26557.1"
                     /translation="MHVFDVIVVGGGISGGLPAATYLQKAGLDVAIIEARPELGNFCP
                     THETWPETLDSPHASINFSGNSPAIVDLELESRYGYRIGTTPVVLGTAHTDGTNCLIC
                     YDPSLTAASFARHSAADGETIFGIQNRVLEKMVEMNELAFYSPHPDAAKFEQVLRICS
                     YVLGRPLEELSSMTAYELLELTFESDKARQTILCPVALHLQGAPLARGQGAFAVALSL
                     FYTTAVAIGGNEALVDAMSRCFLDHGGTILTNCPVERIEVIDGKARSVVLAPEAVFPE
                     ARFEARHAVVSNVGARKTLELVGEDTMASVDSRLAAKMRHWKMDERGSTVTSWLIDGD
                     MPWGSAAYDPMVEKAHLMYRAYDSWDAAKRYVTSMINNDTWAAFGNMIEILDYGKVDP
                     NAVSPTGQRVIRGEEAIPYPLRQHGGPEAWDGPIRDEMLTRRHDVMESIAPGFKDRIL
                     EAYQWTPVDIWRLNPAAVYGQVLGGDFSEDQWMLDRMPYRMPVRGLYMSNAVWPLGLT
                     WMAAGYNAAQVVAEDLGVRNQAWWSARPVAWFLDNIGRLLEPLELAAAGARR"
     gene            7241..8884
                     /locus_tag="VLC50_03475"
     CDS             7241..8884
                     /locus_tag="VLC50_03475"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HSF26558.1"
                     /translation="MTTDKYDVIIVGAGPNGLTCAAYLARAGASVLVLDKRFELGGTM
                     ATDDYSTPFHFNIAQFGLPVGAAAPPYDDLGLADLGVRFIEPDVAAAFVPASGGDPLV
                     VHRDGHGLGELAETVEAARHTVLPLLYASPAPLSDVEAALGRGEGKRVLDLAVLSPAE
                     LVESVSDSRAGGLLRLLCVTAGFANPDERLGVLGAYTVLSRLSPVLVAGGSKSLALGL
                     FRAGASAGAHYRMVADVLAIEVVDGGVRVGCRDGREFGARTVVSTLDPRTTFLDLLSE
                     DVVPAPIRQAAGDWAYDENGPFTAHFGIKGEPPRLASDEASAALMQVLGFDDAASVAD
                     TLGAVRRGELADEPRGVLSVTTRHDPRLAAPGPYGPLHTLRFVTPVPRNSPEGPWDRE
                     RAVHRARCYDALTRHTSGLEDARLLFAFADSPEDIERRFRTTRNGSLRQGRLAEHQTF
                     TDRPHPQVSSTATPIPGVFLGGGGVHPGIPGTLAGGYLAAAAVCDHLRLTHWWPTPAV
                     VEQARESGALPTSLLPPKREASATVPVQGRARAERVAFG"
     gene            8978..>9727
                     /locus_tag="VLC50_03480"
     CDS             8978..>9727
                     /locus_tag="VLC50_03480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF26559.1"
                     /translation="MRARIARLGLLGAVVGALVIALVATPAAADPTYPSQRQVDAAKA
                     KVASTAATVGRLEAQLVAADARLEELGVQAAQAVEAYDGARWALQEATADAEEAAARF
                     GAAQGQVRDAEDALGAYAAAVYRSGGDLAAVGSLLDAAGPAQLAHRMSMLARLGEAQQ
                     QVVEDLQVSRAAAAGLSAQAEAARTRQQQAVERVAAAKAAAQERVAEQKRSVAAIQAQ
                     RVELVAALAKARRTSVALEQARAEGIAKERAE"
BASE COUNT         1376 a         3433 c         3501 g         1417 t
ORIGIN      
        1 tcatcgcgga ggagcgtggc atgaggggcg gtcatcatcc gctgctgttc cagctgctca
       61 acgagatgga cgggctggcc gaggacgccg acgtcgtgtt cgtgctcacc accaaccggg
      121 ccgacctgct cgagccagcg ctggccgctc gtcccggtcg tgtcgaccag gcggtcgagc
      181 ttggcctgcc cgatgcggct gcccggcggg cgctgctcga cctctaccgc ggccagctgc
      241 aggtcgaccc gaccgacctc gaccgggccg ccgccgcgac tgacggcgtg accgcgtcat
      301 tcctcaagga gctgctccgc cgcgcggccc tggtcgccgc cgaggacgcg cccggcgcca
      361 tcccggtgct cctcaagggc acacatctcg acgatgccct gcgcgagctg caggactctc
      421 gcaacgccat gactcgtgcc ctgctcggct cggtgccgtc cgtgtgaccg agagtcggtc
      481 caccgacaag taggccttgg caaggcgttc tggcacttgg caaggcgtgc acgccttgcc
      541 aagtgcgcgg acgcccactt ggcgtggtca tctgcggggt agcggcgcgc taggcgaaga
      601 tgcgcaccgg gtgctcgagc aggccgatga aggtgcgcat ccagtcggcg gccagtgcgc
      661 cgtcgatcgc ccggtggtcg accgagaggg tcacccgcat cacggtggca gcgcggatct
      721 cgccgtccac gacgaccggc tcggcctggg cggcgccgac cgcgaggatc gccgactgcg
      781 gtgggttgat gatcgcggcg aactcctcgg tgccatacat cccgaggttg gtgatgctga
      841 ccgcaccgcc ctcgagctcg tcctggcgca gggtgccggc cttggcccgg gcgacgaagt
      901 cctgcacttg cgcggcgacc tcggagatcg gggtcctctc gacggcccgc agcaccgggg
      961 tcaccagccc ccggtcggtg gccacggcca cagcgatgtc caccgaggag aacgaccgga
     1021 tcacgtcgtc gagccagatc gtgttgaccg ccggcacgag catgtgggcc cgcgccaccg
     1081 ccttgatcac catgtcgttg atggagatct tggtggggct tacctcgttg agctcggccc
     1141 gcgcggccag cagcgcgtcg accttggcgg tgccgcgcag gtagaagtgc ggcgtcgtct
     1201 gcttgctctg ggtcagccgg gccgcgatca ccttgcggat gcgtgagggt gccgtgtcgg
     1261 tgaagtcccc agggacagaa gccgaggccg ccggcgccgg cgcggtgacg ggagcagccg
     1321 gagccgtacg acgacccgcg agcgcggtgt cgacgtcgcg gcgcacgatc cgaccgccgg
     1381 ggcctgtgcc gacgatctgg tcgaggccta gcccggcctg cttggccagc cggcgggcca
     1441 gcgggctggc gaagatccgt gcgcccggca gccggtccac caccgcgccg tcggccgggg
     1501 tcgccgcggg tgcggtcgaa gcgggcgcgg gtgccgcggg cgtggccgca tcggccggtg
     1561 tcgcggagcc gttgacctgc gtgggtgccg ccgccggtgc ctcgacgccg aggctgacga
     1621 gcagcgcgtc gaggtcgtcg atgacctcac cgtgatcgcc gaggatcgcg atcggcgcac
     1681 cgacctcgac ctcggcgcca ggggagacca gggtcttgag gatcacgccg tcggcgtcgg
     1741 cctcgacgtc gacgacggcc ttctcggtct cgacggtcgc cagcgtgtcg gtggccgaga
     1801 acgccgtgcc ctcggcgacc ggccagtccg cgaggaccgc ctcggtggca ttggctgcca
     1861 ccgccggcat gcgcagcagt cgggccatga gtcaggactc cgtgatctcg gtgagcttgg
     1921 cgacgacttc ctcgctcttg gcgatcgcgg cccgctcgag caccttgctg atgctcggcg
     1981 aggcctcacc cccggtcacg cgctggatgg ggctgtcgag ccagtcgaag aagcgtcgct
     2041 ggatctcgtc ggccagccag ccgccgtagg aggtgccgag cgcgccctgc tcggcgatga
     2101 ggacgttgtt ggtcttcttg atgctctcgc cgatggtgtc ccagtcgatg ctggcccggt
     2161 cgagccagcg caggtcgatc aggtcggccg agacgtcggg gagcgcgtcg agggcctgca
     2221 ggcagtagtt gaccatcgcc aggtaggaga tgacggtgag ctgcgagccg gtacgacgca
     2281 gcgcggcctt gccgacgggc aggcagtagt cgaggtcgtc gaccgggccc tcgccggtcg
     2341 aggtgtagag gtcgacgtgc tcgagcacca gcaccgggtc cttgcaccgc aacgcgctgt
     2401 tcatcagccc gacgtagtcg aacggcgtcg agggcgcgac gatgcgccaa cccgggttgg
     2461 tcgcaaagac gccggccggg tccatcgagt gctgcgagcc atagccggtg cccatcgcca
     2521 ccttgctgcg cagcaccagc gggatgtcgc tgtgcccgcc gaacatgtgc cgggccttgc
     2581 cgatctggtt gaacagctgg tcggcggcca cccacatgaa gtcggcgtac atgaactcga
     2641 ccaccggctt gaaccggccg tcgagcgcga tgccacatcc cagcccggtg aaggcgttct
     2701 cgctgatcgg cgtgccgaga atgcggtcgg gataggccgc ggccaggccc ttggtcgcgc
     2761 cgttggtgcc gccgctgagc cggtggacgt cttcgccgag cacgatgatc gagtcgtcgg
     2821 tggccatccg gcggcccatc accccggcca ccgcgtcgat gaacttctgc tcggccagct
     2881 cgccggtgaa ggtgtcgcgg tcggcgaatc gcgcccccgc gaactcgctg aggtcaccgc
     2941 gcacgccgac gtcgcggaag gacgggtcgg gccactcgct cggcttgatc cgccgctgcc
     3001 cgggcttgcc gtcgggatcg ggctcgagca gcacgtcgcc gagctcggcc atcaacgtct
     3061 tggcccgcgc ccgcaggtcg tcgatctggt cctgggtgag gatctggcgg cgtacgacgt
     3121 gcccggccag cgcctcgatc gggtcacggg cgcgccactc ccgctcctcc tccttcgagc
     3181 ggtagccgaa ggccgagccg gggaacgggc cgttctggtg gaagtagcga tagacgtcgg
     3241 cctcgatcag ggtcgggccc ttgccggccc gcatgtgcgc cagcgcctcc tgcatcgcca
     3301 ggtagaccgc gagcgggtcc atcccgtcga ccttccagct cgggatgttg aaccccggcc
     3361 cgcgcgccga cagccgcggc tcgccggtgg cctcgaagac ggtggtggac acggcgtact
     3421 ggttgttctc gatgaagaag cagaccggca ggtgccaggc cgcggcgagg ttgaagctct
     3481 cgagcgtgga gccgatgttg gccgccccgt cgccgaagta ggtcaccgag accgcgtcgg
     3541 tgccggcgtg gcgatcggcc caggcgaacc cgaccgcctg cgggaccccg ccgccgacga
     3601 tggcgttggt gcccatcgcg ccggcttcct tccactgcag gtgcatggac ccgccccggc
     3661 cgctgctgaa gccgcgggcc agcccgcaga tctcggcgag cgtggtcagc aacgcctggc
     3721 gcacgtcctc ggggaacggc gcggtcgggt cgaggccctt gggggccagg tgggtcagcg
     3781 ccttggcgag gaactggtgg tggccgcggt gcgagccgtt gacaccgtct gcgctggtga
     3841 gcccgatgat cgagccgacc gcgccgccct cctggccgat gctcgagtgc gccggaccgt
     3901 gcacgagccc cgcgccggcc agctcgagga cgtactcctc gaaggtgcgg atcagcacca
     3961 gctggctgag catccctgtc agcaggcccg ggtcggcggc gtcccagtca tcggcggtgg
     4021 cgacgatctg gatccacggt gccgccggtt ccagccggtt gcgctgcgtc atggcactct
     4081 ccacggggtc tgagtctcgg gccagcctgc cacagtcgtg gatccaatat ctagccctga
     4141 tcggtcatcg actcgcagcg gagcgctggc tatcctgtct gggatccaat ccggagatcg
     4201 acgtgaggtg ggtcacgaca tggcggacgc gaccggactg cccgggctga gcggggttga
     4261 ccacatcggc ttcaccgtgc ccgacctcga ggaggccacc cggttcctca tcgacgtgct
     4321 cggctgcgaa tacatgtaca cgctgggccc gttcagcgac tccgacggcg actggatgag
     4381 cgagcacctc aacgttgacc cccggacggt gatgcagcag ctgcacttct tccggtgcgg
     4441 cgggcaggcg atcttcgagg tctttcagta cgccgccccc gaccagcgcg cccagggccc
     4501 ccgcaacagc gacatcggcg ggcaccacgt cgcgctctac gtcgacgacc tggacaaggc
     4561 cgtcgactac ctcaaggcca agggcgtgcg gatgctcggc gagcccaccg cgagcaaggg
     4621 ccccagcctg gggcagcggt gggtctactt cctcgccccg tggggcatgc agttcgagct
     4681 ggtctcctac ccggacggca aggcgttcga caagcaggcc ggcgacgggg ccgcgaccag
     4741 ttcatgatcg ggtcgaaggt cgaggcggcc agccagcggg tggccgacta cctgcgccag
     4801 gccatcctca ccggggagat cggcccgggc gagcgcatcc gacagcgcga ggtggccgag
     4861 cggctcggcg ccagccggct gccggtgcgc gaggcgctgc gcatcctcga ggccgagggc
     4921 ctgaccgagc acgagatgaa caagggcgcc cgggtgccgg tgctcgacct gcacgaggtg
     4981 caggtggtct accagacccg cgaacgcctc gagccgctcg cgctgatcga gagcatgccg
     5041 cgcctgaccg ccgacgacct cgccgagctg cgccggatcc aggaccgcat cgcggccgcc
     5101 cccgacatca ccacattcct cacgttggac cggcagttcc acctgctgac ctactccgga
     5161 tgctcgaccg gctctctcaa ctcgatcgtg acccggctgt ggaactccac ccagcactac
     5221 cggcgcgcct tcgtcacgat ctccgggccg ggacggatgt ggatcgtcaa cgccgagcac
     5281 gagctgctcc tcgacgcgat cacccgggcc gacacggttg acgccgagcg gttcctcagc
     5341 gggcacatcc gccgcacccg gatcgagctg gcccagcacc cggaggcctt cgccggctga
     5401 gccccgggcc cccgacccct tggctgcgtc ggggggtcgg cgtaggttgg caacgactcc
     5461 gagacgtggc cactcatgct tcccctgcac gcccgctcgc ccccgcactg gtcggcttcg
     5521 cacccgcccc acaccactgc gaggagtcag ccatgcacgt gtttgacgtg atcgtggttg
     5581 gcggcgggat ttcggggggc ctgcccgccg cgacctatct gcagaaggcc ggtctggacg
     5641 tcgcgatcat cgaggcgcgc cccgagctcg gcaacttctg ccccacccat gagacctggc
     5701 cggagacgct ggacagcccg cacgcatcga tcaacttctc cggcaactcc ccggcgatcg
     5761 tcgacctcga gctggagtcg cgctacggct accggatcgg tacgacgccg gtggtgctcg
     5821 gcaccgcaca caccgacggc accaactgcc tgatctgcta cgacccgtcg ctgaccgcgg
     5881 ccagcttcgc gaggcactcg gccgccgacg gggagaccat cttcggcatc cagaaccggg
     5941 tgctggaaaa gatggtcgag atgaacgagc tggcgttcta cagcccgcac ccggacgccg
     6001 cgaagttcga gcaggtgctg cggatctgct cctacgtgct cggtcggccc ctcgaagagc
     6061 tctcgtcgat gaccgcctac gagctgctcg agctgacctt cgagtccgac aaggcgcgcc
     6121 agacgatcct ctgcccggtc gcgcttcacc tgcagggcgc gccgctggcc cgcgggcagg
     6181 gtgcgttcgc ggtggccctg agcctcttct acacgaccgc cgtcgcgatc ggtggcaacg
     6241 aggcgctggt cgacgcgatg agccgctgct tcctcgacca cggcggcacg atcctcacca
     6301 actgcccggt cgagcggatc gaggtcatcg acggcaaagc ccgctcggtg gtcctggcgc
     6361 cggaggcggt gttccccgag gctcggttcg aggcacgcca cgcggtggtc tccaacgtcg
     6421 gcgcccgcaa gacgctcgag ctggtcggcg aggacacgat ggcctcggtc gactcccggc
     6481 tggccgccaa gatgcgccac tggaagatgg acgagcgcgg ctcgaccgtc acctcgtggc
     6541 tcatcgacgg cgacatgccg tggggttcgg cggcctatga cccgatggtc gagaaggccc
     6601 acctgatgta tcgggcctac gactcgtggg acgccgccaa gcgctacgtc acctcaatga
     6661 tcaacaacga cacctgggcc gcattcggca acatgatcga gatcctcgac tacggcaagg
     6721 tcgaccccaa cgccgtctca ccgacgggcc agcgggtgat ccggggcgag gaggccatcc
     6781 cttacccgct gcgtcagcac ggcgggcccg aggcctggga cggcccgatc cgcgacgaga
     6841 tgctcacccg ccgtcacgac gtgatggagt cgatcgctcc cgggttcaag gaccgcatcc
     6901 tcgaggccta ccagtggacc ccggtcgaca tctggcggct caacccggcc gcggtctacg
     6961 ggcaggtgct cggcggcgac ttctccgagg accagtggat gctcgaccgg atgccctacc
     7021 ggatgccggt gcgtggcctc tacatgtcca acgcggtctg gccgctgggg ctgacctgga
     7081 tggccgccgg ctacaacgcg gcccaggtcg tggccgagga cctcggcgta cgcaaccagg
     7141 cctggtggtc ggcccgcccg gtcgcctggt tcctggacaa catcggtcgg ctgctcgagc
     7201 ccctcgagct ggccgccgcc ggggcacgga ggtgatcccg atgacaaccg acaagtacga
     7261 cgtgatcatc gtcggggccg ggccgaacgg cctgacgtgc gccgcctacc tcgcccgggc
     7321 cggggcgagc gtgctggtgc tcgacaagcg gttcgagctc ggcggaacca tggccaccga
     7381 cgactactcg acgccgttcc acttcaacat cgcgcagttc gggctgccgg tcggcgcggc
     7441 ggcaccgccg tacgacgacc tgggtctggc cgacctgggc gtgcggttca tcgagccgga
     7501 cgtggcggcg gcgttcgtgc cggccagcgg cggcgacccg ctggtggtgc accgggacgg
     7561 gcacgggctc ggcgagctgg ccgagaccgt cgaggcggcc cggcacaccg tgctgccgct
     7621 gctctacgcc tcgccggcgc cgttgagcga cgtcgaggcg gcgctgggtc gcggggaggg
     7681 caagcgggtg ctcgacctgg ccgtgctcag cccggccgag ctcgtcgagt cggtcagcga
     7741 cagccgcgcc ggtggcctgc tgagactgct gtgcgtgacg gctggcttcg ccaacccgga
     7801 cgagcggctg ggcgtgctcg gcgcctacac cgtgttgagc aggctgagcc cggtgctcgt
     7861 ggccggcggg agcaagtcgt tggccctcgg gctgttccgg gccggggcca gcgcgggcgc
     7921 gcactaccgc atggtcgccg acgtgctcgc gatcgaggtc gtggacggcg gggtgcgagt
     7981 gggctgccgc gacgggcggg agttcggcgc ccgcacggtg gtgtccacgc tcgacccgcg
     8041 gaccaccttc ctcgacctgc tgtccgagga cgtcgtaccc gcgccgatcc gccaggcggc
     8101 aggcgattgg gcctacgacg agaacgggcc gttcaccgcg cacttcggca tcaagggcga
     8161 accgccgcgg ctggccagcg acgaggcctc ggcggcgctc atgcaggtcc ttgggttcga
     8221 cgacgcggcc agcgttgccg acaccctcgg ggcggtgcgc cggggtgagc tggctgacga
     8281 gccacgcggc gtcctctcgg tgacgacccg gcatgacccc cggctggccg cgccgggacc
     8341 ctatgggccg ttgcacaccc tgcggttcgt gacaccagtg ccgcgcaaca gcccggaagg
     8401 gccgtgggac cgggagcggg ccgtgcaccg ggcccggtgc tacgacgcgc tgacccggca
     8461 cacaagcggg ctggaggacg cgcggctgct gttcgcgttc gccgacagcc ccgaggacat
     8521 cgagcggcgc ttccgcacca cccgcaacgg ttcgctgcgt cagggtcggc tggccgagca
     8581 ccagaccttc accgatcgcc cgcacccgca ggtgagctcg acggcgacgc cgatcccggg
     8641 ggtcttcctg ggcggtggcg gcgtgcaccc cgggatcccc gggaccctgg ccgggggcta
     8701 cctcgccgcg gccgccgtct gtgaccacct gcggctgacc cattggtggc ccacgccggc
     8761 ggtcgtcgag caggcccggg agtcgggggc cctgccgacg tcgctgctgc cgcccaagcg
     8821 ggaggcctcg gcgacggtgc ccgtgcaggg ccgggcaagg gccgaacggg tggcatttgg
     8881 gtgaaccggt tcccaaggtg gtgacagagg gttagcctcg acggactcct ggtgacgggc
     8941 tggcgacccg tggccacttc cccgtcggag gccacccatg cgcgcacgca tcgcccggct
     9001 cgggctgctg ggcgccgtcg tgggtgcgct ggtcatcgcc ttggttgcca ccccggcggc
     9061 cgctgacccc acttatccga gccagcgtca ggtcgacgcc gccaaggcca aggtggcctc
     9121 caccgccgcg accgtgggcc gcctcgaggc ccagctcgtg gcggccgatg ctcggctcga
     9181 agagctcggt gtgcaggcgg cgcaggcggt cgaggcgtac gacggggccc ggtgggcgct
     9241 gcaggaggcc accgccgacg ccgaggaggc cgccgcccgc ttcggtgccg cgcagggaca
     9301 ggtgcgcgac gccgaggacg cactgggcgc ctatgccgcg gcggtctacc gctccggcgg
     9361 tgacctcgcc gcggtgggca gcctgctcga cgcggccggc ccggcccagc tggcccaccg
     9421 gatgagcatg ctggcccggc tcggcgaggc gcagcagcag gtcgtcgaag acctgcaggt
     9481 ctcccgcgcg gcggccgccg ggctgtcggc acaggccgag gcggcccgga cccgccagca
     9541 gcaggcggtg gagcgggtgg ccgccgccaa ggccgcggcg caggagcggg tggccgagca
     9601 gaagcgctcg gtggccgcga tccaggccca gcgggtcgag ctcgttgcgg cgctggccaa
     9661 ggcccgccgc acctccgtgg ccctggagca ggcgcgggcg gaagggatcg ccaaggaacg
     9721 ggccgag
//
LOCUS       DATIBB010000095        17358 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_1627941, whole genome shotgun sequence.
ACCESSION   DATIBB010000095 DATIBB010000000
VERSION     DATIBB010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 17358)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17358)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17358
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1627941"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            69..560
                     /locus_tag="VLC50_03485"
     CDS             69..560
                     /locus_tag="VLC50_03485"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSF26560.1"
                     /translation="MSRPVASPSEWTDSAAATSNVSVPLQAARPEELSRSVWLEAHLT
                     APHDARELVRALATEWGLANPTLDDAVSVASELVTNAVVHAHSRFQLSVSMGSEHLTI
                     RVASTFRRSDDSSTETPRADSTDHGVGLWIVDHLAESWGTLNTRQGKAVWASLALTAT
                     NRT"
     gene            complement(561..1064)
                     /locus_tag="VLC50_03490"
     CDS             complement(561..1064)
                     /locus_tag="VLC50_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013838019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dsRBD fold-containing protein"
                     /protein_id="HSF26561.1"
                     /translation="MKASVGDPIVIVSNQLDRPVREGEILEVRGPGGSPPYLVRWADT
                     GHTGLCFPGPDARIEHRGGSATGTGVPTPRTEAEQGPVLRWTVDVQVSETGDDTTARV
                     VLRQDTATQLEARGRAHRNPSDDLVPQIGAEVAVARALRRLADQLLSVAASDIAEVEG
                     HPVQLPR"
     gene            complement(1142..1951)
                     /locus_tag="VLC50_03495"
     CDS             complement(1142..1951)
                     /locus_tag="VLC50_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009187653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HSF26562.1"
                     /translation="MAHLIAVDDAADPRLHEYLGLTDAALRTRREPAEGMFVAESEKV
                     IRRAVAAGYPVRSLLTAPRWVAPLADLLDPLDVPVYVAGPEVLAAVTGFTVHRGALAV
                     MARKPLPDPHELVRDARLVVVLEGVVDHTNVGAAFRSVAGLGADAVLVSPRCADPLYR
                     RSVRVSMGTVLQVPWTRLAPWPAGLDDLRIAGFDVVGLALAEDALDLASYAAQAPQRV
                     ALVLGTEGDGLSHRTLARCDHTVRIPMAHGVDSLNVAAAAAVAVHALVHRG"
     gene            complement(1955..2734)
                     /locus_tag="VLC50_03500"
     CDS             complement(1955..2734)
                     /locus_tag="VLC50_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014981136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HSF26563.1"
                     /translation="MTLIEVVAILLAGVAAGTINTIVGSGTLITFPTLLAFGYSPIVA
                     NISNNIGLVPGGVSGTIGYRRELVGQGGRLRQLAPLSLAGGASGALLLFILPPAAFAA
                     IVPALLAVAVVLVIIQPWMKATLARRRAGREVTPRGARTNTLLVQVGVFLAGAYGGYF
                     GAAQGVLLVGLLGMLLPETLQRINAVKNVLSLVVNGVSTAVFVTVAFDQIDWLVVLLI
                     ASGSMIGGVIGARVGRRMPAAVLRAVIVIVGTVAIVKIVTA"
     gene            complement(2832..3992)
                     /locus_tag="VLC50_03505"
     CDS             complement(2832..3992)
                     /locus_tag="VLC50_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HSF26564.1"
                     /translation="MDPAVITAVVLVIVALFVITTLAKTVRIVPQASSGIVERLGRYT
                     RTLDAGLHILMPFFDKVRVMIDLREQVVSFPPQPVITEDNLVVSIDTVIYYQVTDPKS
                     ATYEIANFIQGIEQLTVTTLRNVIGGMDLERTLTSREEINGALRGVLDEATGKWGIRV
                     NRVELKAIDPPASIQDSMEKQMRADRDKRATILTAEGERQSAILTAEGDKQSAILRAE
                     GDREALILRAQAERESQILRAQGQAQAINTTFQAIHDGRVDQALLSYQYLQMLPQIAQ
                     GESNKLWIVPSELGKALEGLGGLLGGEAVPASAPGKRPGLLHAPSAPSINHATAETEA
                     RVDESLATANAEVQAAIAAAQDSASALGTGSKTPSEPAQGPETDSTTPGATP"
     gene            complement(3996..4445)
                     /locus_tag="VLC50_03510"
     CDS             complement(3996..4445)
                     /locus_tag="VLC50_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020573771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HSF26565.1"
                     /translation="MEWLGDHAWVLWIALAVVFGIVELTTLDLLFLMLAGGALAGGLV
                     ALTAVPAVGQVLVALAVSIALLGVVRPVALRHLRTPIETRTGVAALVGRQAVVVERID
                     RHGGRVKLGGEVWSARSFDPHHEIEYGQTVDVVEIEGATAVVYESEL"
     gene            complement(4503..5294)
                     /locus_tag="VLC50_03515"
     CDS             complement(4503..5294)
                     /locus_tag="VLC50_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006591036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSF26566.1"
                     /translation="MGDVLRFSGVSVVRGGATLLDSIDWVVREDERWVVLGANGAGKT
                     TLLELASATLHPSSGTAVILGEQMGRVDVFELRPRIGLASAALAHRLPARERVADVVV
                     TASYAVVGRWRESYESLDHARATHLLATLGVGHLADRRYGTLSEGERKRVQIARALMT
                     DPELMLLDEPAAGLDLGGREDLVHRLGTLAGDPTSGTLVLVTHHVEEIPSGFTHVMLL
                     RQGRVVASGPIETTLSAQALADTFGIPLVLERHGPRWSARAAVAR"
     gene            5393..6145
                     /locus_tag="VLC50_03520"
     CDS             5393..6145
                     /locus_tag="VLC50_03520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF26567.1"
                     /translation="MARTTTSAHPTTLAAVRLTAWGNAVLAGRVSLDQAADAIAEGGH
                     HRVCLLPAEPDQVNLAYGLGVLRRLGVSRLRAVLPAPGDPAGLPGPARFNKAALAAGA
                     AVILVAPRPMGALPSVRGESTDWMVSDVAGTGEGAAPDLVSAERELTRAVAAAAEELA
                     RLDVARWRPDLAAGLQEVRSGDCRVGLPVGHPQRAVGLAAQAIRMLRLVDLAGHDDGG
                     ALVGSQVRARSAVLSQLHGCARRALAASATGV"
     gene            6172..7413
                     /gene="glgC"
                     /locus_tag="VLC50_03525"
     CDS             6172..7413
                     /gene="glgC"
                     /locus_tag="VLC50_03525"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014445308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HSF26568.1"
                     /translation="MATPKVFAIVLAGGEGKRLMPLTTDRAKPAVPFGGVYRLIDFAL
                     SNLVNAGYLQIVVLTQYKSHSLDRHISKTWRMSPLLGNYVTPVPAQQRIGKRWFAGSA
                     DAIYQSLNLIYDERPDHVIVFGADHVYRMDPRQMVDCHVASGAGATVAAIRQPIEMAR
                     QFGVIQTRPEDPARIAGFLEKPSEVEGLPDAPEQIYASMGNYVFETSTLLDAVTTDAA
                     NDESNHDIGGDIIPMLVDRGAVGVYDFRDNVVPGSTPRDRDYWRDVGTLDSYYDAHLD
                     LISIEPIFNLYNRRWPIYTSMGTWPPAKFVHGWHGRVGQAINSMVSPGAVVSGARIES
                     SVLSPDVHVHSWATVTDSVLLDGVRVGRNAVIRRAIIDKNVVVPEGASIGVDLEHDRD
                     RGFTVTDSGIVVLGKGAQVPV"
     gene            7474..8676
                     /gene="serB"
                     /locus_tag="VLC50_03530"
     CDS             7474..8676
                     /gene="serB"
                     /locus_tag="VLC50_03530"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019631796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HSF26569.1"
                     /translation="MLPAPRPRTLLVTLTGHDRPGVTSALFEALVRHRVEVVDLDQVV
                     IHGQLVLAVALGGVRDEPAVRKALVAAADALDMQLEVHPGDEAAPPRRGRSHVTVLAR
                     SLTPASIASIAGRIADLGANIDRIVRIAHYPVTAVELLVSGIAPARLRESLSALAAAT
                     GVDVAVQPAGLLRRAKRLVVMDVDSTLVQGEVIEMLAEHAGCAERVAELTAAAMRGEL
                     DFAAALRERVALFAGLDAAALDAVRREVRLAPGARTLVRTLKRLDHKVGIVSGGFTQV
                     TDTLVAELGLDFAAANTLEVVDGRLTGRLVGPIVDRVGKAAALERFARQAGVPLGQTV
                     AIGDGANDLDMLALAGLGVAFNAKPIVRQAADTSVSAPYLDSVLFLLGITREEIEAAD
                     AADGLVTT"
     gene            complement(8673..9446)
                     /gene="fabI"
                     /locus_tag="VLC50_03535"
     CDS             complement(8673..9446)
                     /gene="fabI"
                     /locus_tag="VLC50_03535"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018635520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004318 - enoyl-[acyl-carrier-protein]
                     reductase (NADH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HSF26570.1"
                     /translation="MGGILDGKRILVAGVTLDTSIGFAVARVAQEQGAEVVISNFGRA
                     LTITRTIAKRLPTEPPVIELDVTDPEHLAGLADKLREHVDGLDGVVHSIAFANPETAL
                     GGKLLTTPWADVAQAVQVSAFSLASLTTAALPLMSKGGSVVGLTFDATVAWPAYDWMG
                     VAKAGLESCARYLARDLGPRGVRVNLVSAGPLRTLAAKSIPGFEDLESMWSGRSPLGW
                     DQADADPTARGVVALLSDFFPATTGEIVHVDGGFHAMGA"
     gene            complement(9514..10230)
                     /gene="fabG"
                     /locus_tag="VLC50_03540"
     CDS             complement(9514..10230)
                     /gene="fabG"
                     /locus_tag="VLC50_03540"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005266756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HSF26571.1"
                     /translation="MTTVEGRSVLVTGGNRGIGLAIAQAFAAHGDRVAVTSRSGSAPE
                     GLHAVAADVTDPSSLDAAFASVEAAHGPVEILVANAGITRDQLVMRMADDDFTAVVDT
                     NLAGAWRVAQRATKGMVRLRRGRMIFISSVVGMLGSAGQVNYAASKAGLVGLARSLAR
                     ELGSRAITANVVAPGFVETDMTAALPDTLRTTYQGQIPLGRFASSDEVARVVHFLASD
                     EAAYITGAVIPVDGGLGMGH"
     gene            complement(10318..10530)
                     /locus_tag="VLC50_03545"
     CDS             complement(10318..10530)
                     /locus_tag="VLC50_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012921326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin"
                     /protein_id="HSF26572.1"
                     /translation="MANRTYRVTEIVGTSPEGIDAAVRNGLARAAQTLRHIDWFQVGE
                     IRGHVVDGQVDHFQVGMKVGFRLEDS"
     gene            10597..10920
                     /locus_tag="VLC50_03550"
     CDS             10597..10920
                     /locus_tag="VLC50_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013883927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3099 domain-containing protein"
                     /protein_id="HSF26573.1"
                     /translation="MTSPDGERGKPTVYRITDARKGITEDVNERQRRYLISMGIRTVC
                     FVLALVTSGWLRWAMIIAALLLPYIAVIIANAGREQAPAPLDTDLHEDPPSLPGGRGE
                     DPPTA"
     gene            11067..11324
                     /locus_tag="VLC50_03555"
     CDS             11067..11324
                     /locus_tag="VLC50_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010987856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF26574.1"
                     /translation="MTDQPPAPAESIPPTCSRQGCRAPATWVLVWNNPKVHTPERRKT
                     WSACDEHREYLTYYLDRRTFLIEVVARDDWEARTSPQGPPS"
     gene            complement(11329..12336)
                     /gene="moaA"
                     /locus_tag="VLC50_03560"
     CDS             complement(11329..12336)
                     /gene="moaA"
                     /locus_tag="VLC50_03560"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007453785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HSF26575.1"
                     /translation="MLSAPAGQLVDRFGRVGRDLRVSLTDRCNLRCVYCMPAEGLQWL
                     PKPQLLTDDEIVRLVRIAVELGITSVRFTGGEPLLRRGLPDIVRRIGELEPRPSMALT
                     TNGIGLDRLAQPLADAGLDRINVSLDTLDREQFAALARRDRLDDVLAGLAAAARSGLA
                     PVKVNAVVMREINYASAPELLRWCLEQGYQLRFIEQMPLDPQHGWDRSTMVTATEVLE
                     LLSQHFELTPEGESVRGSAPAETWIVDGGPGKVGIIGSVTRPFCGACDRVRLTADGQL
                     RNCLFARGESDLRAPMRAGASDDELAEIFKSTMWAKRAGHGIDDPNFLQPSRPMSAIG
                     G"
     gene            12421..13197
                     /locus_tag="VLC50_03565"
     CDS             12421..13197
                     /locus_tag="VLC50_03565"
                     /inference="COORDINATES: protein motif:HMM:NF014193.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SURF1 family protein"
                     /protein_id="HSF26576.1"
                     /translation="MHRTLLTRRWLGLLVLCLLLATVCVRLGFWQLHRFEDHRAANQR
                     VTQQISADPVPLQTLLPVGRSVGPAQEWRRVTASGEYDVTRQLLVRQRPLNGANGYHV
                     LTPLVLADGSALIIDRGWVPAGASATTSPAVPAPPDGQVTVTARLRPSETGPLQQAGL
                     PPGQIRRIAVAEIAADLPYPVADGYAELVGQTPAPAQELSSLPLEQKSTALNLAYAVQ
                     WWLFAAIIVGGWYFLGRREALDEQARRGADLDPAQTAVGG"
     gene            13214..15040
                     /locus_tag="VLC50_03570"
     CDS             13214..15040
                     /locus_tag="VLC50_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020547534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HSF26577.1"
                     /translation="MASRIEDYALVGDMESAALVGRNGSIDWLCVPRFDSPAIFAALV
                     GSADNGHWIIAPVGAGDATRRRYRPDTLILEQEWDTADGRLRLLDFMPARDKAPNLVR
                     IVTCLSGEVAVRSVLRLRFDYGRVLPWVTHRDGHLSAVAGPDAVNITSTVPLHGRDFA
                     TQSEVTLQAGQSASFVLTWHPSYEPEPSPVEPDEALAQTEQFWKDWVARSTYEGPYRE
                     AVVRSLITLKALTFEPTGGLVAAPTTSLPEDLGGTRNWDYRFCWLRDASLTLRALIAT
                     GHTEEAEAWRGWLLRAIAGDPADLQIMYGLAGERRLPEWSPEWLVGYEGSTPVRIGND
                     AAVQRQLDVYGEVLDTLDLAGAAGLSSDAATWAIQVALLDWLEQHWQEPDEGLWEVRG
                     PRRQFVHSKVLAWVAFDRAARMAHALGHAQHAARWSRVRDEIHEEVCQKGYDADRGTF
                     TQYYGSAGLDAATLLIPMLGFLPGDDPRVLGTIAAVEATLAVDGFVLRYDTHGSVDGL
                     PGHEGAFLACSFWLATALHQSGRTAEATALFERLLGLCNDVGLLSEEYDPRYQRLVGN
                     FPQAFSHIGLLNTAITLAHPPGQAPFLVRHQRGTRKGDPRPR"
     gene            15092..15856
                     /locus_tag="VLC50_03575"
     CDS             15092..15856
                     /locus_tag="VLC50_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014151753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HSF26578.1"
                     /translation="MDLGLDGQVFIVTGGTRGLGRATVDVLVAEGARVVVCSRDGAAV
                     AKTSAEHGGSDRVLGIAGDLAEAGTETRLVAAAIARYGRLDGALVSVGGPPAGPSHAL
                     TDEVWRSAFESVFLGALRVARAVVKALPAEGGSIAFVLSSSVRNPIAGLAASNGLRPG
                     LAMVAKTMADELGARNVRVNGLLPGRIATDRLRELDERTGRPQAARRDSEARIPLGRY
                     GQPEEFGRVAAFVLSPAASYLTGAMVPVDGGAIRAL"
     gene            complement(15860..>17358)
                     /locus_tag="VLC50_03580"
     CDS             complement(15860..>17358)
                     /locus_tag="VLC50_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019633781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSF26579.1"
                     /translation="VSRLNSDVIGAQQALTSTLSSVVSNVVGLVLVLVAMLILSWQVT
                     LASLLLLPVFVIPAKLVGRRLAVITREAMQLNAAMSQTMTERFNVSGALLVKIFGRLP
                     DEQAAFTDKAARVRDIGVIQAMYSRVFFTALTLVAALATALVYGIGGLLAVDGELAVG
                     TLVALTALMTRLYGPLTALSNVHVDVMTALVSFDRVFEVLDLPPMVADRPGATTLPPG
                     AASVELDHVAFRYPAADEVSLASLESVAVLDSAPPTQVLHDVSFVAAPGELVALVGPS
                     GAGKTTITALVSRLYDVTSGAVRIGGHDVRDVTQESLHRTVGVVTQDAHMFHDTIRNN
                     LLFARPEASEAELVEACRSAQIWDLVASLRDGLDTVVGDRGYRLSGGEKQRLAIARLL
                     LKAPSIVVLDEATAHLDSESELAVQQALATALAGRTSIVIAHRLSTVRHAEQILVVDD
                     GRIVERGRHADLLAAGGLYAELYRTQFDRGQGPPPAEAPPDVIFGRLG"
BASE COUNT         2489 a         6243 c         6202 g         2424 t
ORIGIN      
        1 cgggcccgcc gaggtccggt cttggccggc gttctcgagg tccgccaacc ggccgaggtg
       61 aggtggcaat gagccgaccc gtcgcctcgc cctcggagtg gaccgactcc gctgccgcga
      121 ccagcaacgt ctcagtcccc cttcaggccg cgcggcccga ggaactctcc cgaagcgtgt
      181 ggctggaggc gcacctgacc gcgccgcacg atgcacgtga actcgtgcgt gcgctggcca
      241 cggagtgggg tttggccaac cccacccttg acgacgcggt cagcgtggcc tcagagctgg
      301 tgaccaacgc tgtggtgcat gcccactcgc ggtttcagct gtcggtctcg atggggtccg
      361 agcacctgac catccgagtc gccagcacct tccggcggtc cgacgactcc agcaccgaga
      421 cgcctcgggc cgactccacc gatcatggtg tcggcctctg gatcgtcgac cacctcgctg
      481 agagctgggg cacgctcaac actcgccagg gcaaggccgt gtgggcatcg ctcgccctga
      541 cggccacgaa tcggacctga tcagcgcggc agctggaccg ggtgcccctc gacctcggcg
      601 atgtccgagg cggccacgct caggagctgg tcggccaggc ggcgcagggc ccgggcgacc
      661 gcgacctcgg cgccgatctg cggcaccagg tcgtcgctcg ggttgcggtg cgcgcggccc
      721 cgtgcctcca gctgggtggc cgtgtcctgg cgcagcacga cccgcgccgt ggtgtcgtcg
      781 ccggtctcgg agacctgcac gtccacggtc cagcgcagca ccggaccctg ctcggcctcg
      841 gtccgtggtg tcggcacgcc ggtgccggtg gcggatccgc cgcggtgctc gatgcgcgcg
      901 tcgggccccg gaaaacacag cccggtgtgc ccggtgtcgg cccatcgcac gaggtagggc
      961 ggggacccgc cgggcccgcg cacctcaagg atctcgccct cacgcaccgg tcggtccagc
     1021 tggttggaga cgatgacgat cgggtcgccg acggaggcct tcatgaccca cctcctcatg
     1081 gagagcgctt ggcccaccca acgcggtgtc gacggcggcg ggaagtgccg aaggtccccg
     1141 gtcagccgcg gtgcacgagc gcgtggaccg ccaccgcggc cgcggcggcg acattgaggg
     1201 agtcgacgcc gtgggccatc gggatccgca cggtgtgatc gcacctggcc agcgtccggt
     1261 gggacagccc gtcgccttcg gtgccgagca cgagcgcgac ccgttgcggt gcttgggcgg
     1321 cataggaggc gaggtcgagc gcatcctcgg ccagcgccag cccgaccacg tcgaagcctg
     1381 cgatcctcag gtcgtcgagg ccagccggcc acggagccag ccgggtccac gggacctgca
     1441 gcacggtccc catcgagacc cgcacgctgc gtcggtagag cgggtcggcg caccgggggc
     1501 tgaccagcac cgcgtcggcg ccgagcccgg cgacgctcct aaaggctgca ccgacgttgg
     1561 tgtggtcgac cacgccctcg aggaccacca cgagccgcgc gtcgcggacc agctcgtgcg
     1621 ggtcgggcag cggcttgcgc gccatcacgg cgagcgctcc gcggtggacg gtgaacccgg
     1681 tgacggcggc gagcacctcg gggccggcga catagaccgg tacgtcgagc gggtcgagca
     1741 ggtcggcgag cggcgccacc caccgtgggg cggtcagcag cgaacgcacc gggtagccgg
     1801 cggccaccgc gcggcggatc accttctcgc tctcggcgac gaacatgccc tcggcaggct
     1861 cgcgccgggt ccgcagcgcg gcgtcggtga gcccgaggta ctcgtgcagg cgagggtcgg
     1921 ccgcgtcgtc gaccgcaatg agatgggcca tcggtcaggc ggtcacgatc ttgacgatgg
     1981 ccaccgtgcc gacgatgacg atgaccgcgc gcagcaccgc ggcgggcatc cgccggccca
     2041 cccgggctcc gatcacgccg ccgatcatcg acccgctggc gatcagcagc acgacgagcc
     2101 agtcgatctg gtcgaaggcc accgtcacga agaccgcggt cgacacgccg ttgaccacga
     2161 gggacagcac gttcttgacc gcgttgatcc gttgcagggt ctcgggcagc agcatgccga
     2221 gcagcccgac gagcagcacg ccctgggcgg ccccgaagta gccgccgtag gcgccggcca
     2281 gaaagacccc gacctggacc aggagggtgt tggtccgagc ccctcgtggg gtgacctcgc
     2341 gcccggcccg acgacgggcc aacgtggcct tcatccacgg ctggatgatg acgagcacga
     2401 ccgcgaccgc gagcagtgcc ggcacgatcg cggcgaacgc cgccggcggc aggatgaaca
     2461 gcagcagcgc acccgaggcg ccgcctgcca gcgacagcgg ggcgagctgg cgcaggcgcc
     2521 caccctgacc gacgagctcg cgtcgatagc cgatcgtgcc ggacacccca ccgggcacga
     2581 gcccgatgtt gttggagatg ttggcgacga tgggcgagta gccgaaggcc agcagcgtgg
     2641 ggaacgtgat gagggtcccc gaaccgacga tggtgttgat ggtccccgcg gccaccccgg
     2701 ccagcaggat ggccacgacc tcgatcagtg tcaccgcggt gtcccccagg tcatggcccg
     2761 ctcaggctag ccatgggtcc ccgggtccac gaagccgtcc gaccggcgag acaggtggtg
     2821 cctcggcagg gtcagggcgt ggcgcccggg gtggtcgagt cggtctccgg cccctgggcg
     2881 ggctccgacg gggtcttgga cccggtgccg agcgccgacg ccgagtcctg ggcggccgcg
     2941 atcgcggcct gcacctcggc gttggcggtg gccagcgact cgtcgacccg agcctcggtc
     3001 tcggccgtgg cgtggttgat gctcggcgca ctcggggcgt gcagcagccc gggtcgcttg
     3061 ccgggcgcgg aggccggcac cgcctcaccg ccgagcagcc ccccgaggcc ctcgagggcc
     3121 ttgccgagct cactgggcac gatccacagc ttgttggact cgccctgggc gatctgcggg
     3181 agcatctgca ggtactggta ggagagcagg gcctggtcga cccggccgtc gtggatggcc
     3241 tggaacgtcg tgttgatggc ctgggcctgg ccctgcgcgc ggaggatctg agactcgcgc
     3301 tcggcctggg cccgcaggat cagggcctcc cggtcgccct cggcccgcag gatggccgac
     3361 tgcttgtcac cctcggcggt gaggatggca gactgccgct cgccctcggc ggtgaggatg
     3421 gtcgcccgct tgtcgcggtc ggcgcgcatc tgcttctcca tcgagtcctg gatggaggcc
     3481 ggcgggtcga tggccttgag ctcgacccgg ttgacccgga tcccccactt gcccgtggcc
     3541 tcgtcaagga cgccacgcag tgccccgttg atctcctcac gagaggtcag ggtgcgctcg
     3601 aggtccatgc cgccgatgac gttgcgcagc gtcgtgacgg tgagctgctc gatgccctgg
     3661 atgaagttgg cgatctcata ggtcgccgac ttggggtcgg tcacctggta gtagatgacc
     3721 gtgtcgatgg acacgacgag gttgtcctcg gtgatcactg gctgcggcgg gaacgagacc
     3781 acctgctcgc gcaggtcgat catgacccgg accttgtcga agaagggcat gaggatgtgc
     3841 aggccagcgt caagcgtgcg ggtgtagcgg ccgagccgct cgacgatccc cgagctggcc
     3901 tggggcacga tgcgcaccgt cttggccagg gtcgtgatga cgaacaacgc cacgatcacc
     3961 aggacgactg cggtgatgac tgccggatcc attgctcata gctccgattc gtagacgacc
     4021 gcggtggcgc cctcgatctc gacgacgtcc accgtctgcc cgtactcgat ctcatgatgt
     4081 gggtcgaacg atcgcgccga ccacacctcg ccgccgagct tgacccggcc accgtgacgg
     4141 tcgatgcgct cgaccacaac cgcctgccgg cccaccaggg cggccacgcc ggtgcgcgtc
     4201 tcgatcgggg tgcgcaggtg gcgcaacgcg accgggcgga ccacaccgag cagggcgatc
     4261 gagaccgcca gggccaccag cacctgcccg accgcgggta cggcggtcag tgccaccaga
     4321 cctccggcca gtgcgccgcc ggccagcatg aggaagagca ggtcgagcgt ggtgagctcg
     4381 acgatcccga acaccacggc caaggcgatc cagagcaccc acgcgtggtc cccgagccat
     4441 tccatgcagc gatcctatgc agccgacgtg tcagatcgcc ttacctgagc tccgggcgtg
     4501 ccctagcgcg cgacggcggc gcgggccgac cagcgcggac cgtggcgttc gagcacgagg
     4561 gggatcccaa acgtgtcggc cagtgcctgg gccgacaacg tcgtctcgat cggcccgctg
     4621 gccacgaccc ggccctggcg cagcagcatc acgtgggtga acccgctcgg gatctcctcc
     4681 acgtggtggg tgacgagcac gagggtgccc gaggtgggat cgccggccag cgtgccgagc
     4741 cggtggacca ggtcttcgcg tccaccgagg tcgagcccgg cggccggctc gtcgagcagc
     4801 atcagctcgg gatcggtcat cagggcccgg gcgatctgca cgcgcttgcg ctccccctcg
     4861 gacagggtgc cgtagcgacg gtcggccaga tgaccgaccc cgagggtggc gagcaggtga
     4921 gtggcccgag cgtggtcgag gctctcatag gactcgcgcc accgcccgac gacggcgtag
     4981 gacgccgtga cgaccacgtc ggcgacccgc tcacgggccg gcagccgatg cgcgagtgcc
     5041 gcactggcca gcccgatgcg cggccgcagc tcgaagacgt cgacgcggcc catctgctcg
     5101 cccaagatca cggcggtgcc cgacgacggg tgcagggtgg ccgaggccag ctcgagcaac
     5161 gtggtcttgc cggcgccgtt ggcgcccagc acgacccagc gctcgtcctc ccgcaccacc
     5221 cagtcgatgg agtcgaggag cgtcgcgcct ccccggacga cggagacccc cgagaaccgc
     5281 agcacgtcgc ccatgggccg accagacctc cctcttggtt cttggctccc catacccgcg
     5341 cgatcaggcg ccaacctacg acacgcaaag ccggtcctcc taccctggcc cggtggcccg
     5401 tacgacgacc tctgcccacc cgacgacgct ggctgccgtg cgcctgaccg cctggggcaa
     5461 tgcggtcctc gccgggcggg tgagtctgga ccaggccgcc gacgcgatcg ccgagggtgg
     5521 gcaccaccgg gtctgcctgc tgcccgccga gcccgatcag gtcaacctgg cctacggcct
     5581 gggggttctg cgccgtctcg gcgtgagccg gctgcgcgcg gtgctcccgg cgccgggcga
     5641 ccccgccgga ctgcccgggc ccgcccggtt caacaaggcg gccctggcgg ccggcgccgc
     5701 ggtgatcctt gtcgcgcctc ggccgatggg agcgctcccc agcgtgcggg gcgagagcac
     5761 cgactggatg gtctcggacg tggcgggcac gggcgagggt gcggcacccg acctggtcag
     5821 cgccgagcgc gagctgaccc gcgcggtggc ggccgcggcg gaggagctcg cccgtctcga
     5881 cgtcgcacgc tggcgtcccg acctggccgc gggcctgcag gaggtgcgca gcggcgactg
     5941 ccgcgtcggc ctgccggtgg gtcacccgca gcgcgcggtc ggcctggcgg cgcaggcgat
     6001 ccggatgctg cggctggtgg acctggcggg ccacgacgac ggcggtgcgc tggtcggctc
     6061 acaggtgcgt gccaggtccg cggtgctgtc ccagctgcac ggatgcgctc gccgggccct
     6121 ggccgcatcg gcgaccggcg tctgagcact ccagcggcta aggtcgaaat catggcgacg
     6181 cccaaggtct ttgcgatcgt gctggccgga ggtgagggca aacgcctgat gcccttgacc
     6241 accgatcggg ccaagccggc cgtgcccttc ggcggggtct accggctcat cgacttcgcg
     6301 ctgtccaacc tcgtcaacgc cggctacctg cagatcgtcg tactcacgca gtacaagtcg
     6361 cacagcctcg accggcacat cagcaagacg tggcggatgt cgccgctcct cggcaactat
     6421 gtgaccccgg tgcctgccca gcagcgcatc ggcaagcggt ggttcgccgg ctcggccgac
     6481 gcgatctacc agagcctcaa cctcatctac gacgaacgcc ctgaccacgt gatcgtcttc
     6541 ggggccgacc acgtctaccg catggatccc cggcagatgg tcgactgcca tgtcgcctct
     6601 ggtgccggcg ccaccgtggc cgccatccgt cagccgatcg agatggcgcg ccagttcggt
     6661 gtgatccaga cccggccaga ggacccggcg cgcatcgcgg gcttcctcga gaagccgtcc
     6721 gaggtcgagg ggctgcccga cgcgcccgag cagatctacg cgtcgatggg caactacgtc
     6781 ttcgagacct ccacgctcct cgacgccgtg accaccgacg cggccaacga cgagagcaac
     6841 cacgacatcg gcggcgacat catcccgatg ctcgtcgacc gcggcgccgt gggggtctac
     6901 gacttccgcg acaacgtcgt cccgggcagc accccccgcg accgtgacta ctggcgcgac
     6961 gtggggacgc tcgacagcta ttacgacgcg cacctcgacc tgatctcgat cgagccgatc
     7021 ttcaacctct acaaccggcg ctggccgatc tacacctcga tgggcacctg gcccccggcc
     7081 aagttcgtgc acgggtggca cggccgggtc ggccaggcga tcaactcgat ggtctcgccc
     7141 ggggcggtcg tgtcgggcgc ccggatcgag agcagcgtgc tctcccccga cgtgcacgtg
     7201 cactcctggg ccacggtcac cgactcggtg ctcctcgacg gggtgcgggt cgggcgcaac
     7261 gcggtcatcc gccgcgcgat catcgacaag aacgtcgtgg tgcccgaggg tgcatcgatc
     7321 ggtgtcgacc tcgagcacga ccgggaccgg gggttcaccg tgaccgactc cgggatcgtc
     7381 gtgctcggca agggagcgca ggtgcccgtc tgacctccgg ccccgagcgc ccagcgaagg
     7441 gacggccgcc ggcgggcgat agcctgcgct gacatgttgc ctgccccccg cccgcgcacc
     7501 ctgctggtca ccctgaccgg ccatgaccgc cccggggtca cgagtgccct gttcgaggct
     7561 ctggtccggc accgggtgga ggtcgtcgac ctcgatcagg tggtgatcca cggacagctc
     7621 gtgctggccg tcgcgctcgg tggggtgcgc gacgaacccg ccgtccgcaa ggcgttggtg
     7681 gccgcggccg acgcgctgga catgcagctc gaggtgcacc ctggtgacga ggcggcaccg
     7741 ccacggcgcg ggcgcagcca cgtgaccgtg ctggcccggt ccctgactcc ggcgagcatc
     7801 gcgagcatcg ccgggcggat cgccgacctc ggcgccaaca tcgaccgcat cgtgcggatc
     7861 gcgcactacc cggtcaccgc tgtcgagctg ctggtgtccg gcatcgcgcc ggcccgcctg
     7921 cgcgagtcgt tgtccgccct ggccgccgcc accggtgttg acgtcgcggt ccagccggct
     7981 ggtctcctgc ggcgggccaa gcgcctggtc gtcatggacg tcgactcgac cctcgtgcag
     8041 ggcgaggtca tcgagatgct ggccgagcac gcgggctgcg ccgagcgggt cgccgagctg
     8101 accgcggcgg cgatgcgcgg ggagctcgac ttcgcggctg cgctgcgcga acgggtggcc
     8161 ctctttgctg gtctcgacgc ggcggccctc gacgccgtac gccgagaggt ccgcctcgca
     8221 cccggggccc gcacgctcgt gcgcaccctc aaacggctcg accacaaggt gggcatcgtc
     8281 agcggcgggt tcacccaggt caccgacacg ctggtggccg agctgggcct cgacttcgcc
     8341 gcggccaaca cgctggaggt cgtcgacggc cggctgaccg gccggttggt cggcccgatc
     8401 gtcgaccggg tcggcaaggc cgccgcgctc gagcgcttcg cccggcaggc cggtgtcccg
     8461 ctgggccaga cggtcgcgat cggggacggc gccaacgacc tcgacatgct cgccctggcc
     8521 gggctgggag tcgcgttcaa cgccaagccg atcgtgcgtc aggcggccga cacgtcggtg
     8581 tcggcgccct acctcgactc ggtgctgttc ctgctcggca tcacccgcga ggagatcgag
     8641 gccgccgacg ccgccgacgg cctcgtgacg acctaggcgc ccatcgcgtg gaagccgccg
     8701 tcgacgtgga cgatctcgcc cgtggtcgcc gggaagaagt cactgagcag cgccaccacg
     8761 ccccgggcgg tcgggtccgc gtcggcctgg tcccagccca acggtgagcg ccccgaccac
     8821 atcgactcga ggtcctcgaa gcccgggatg ctcttggcgg ccagcgtgcg caacggtcct
     8881 gccgacacga ggttgacccg gacgccgcgc ggaccgaggt cgcgagcgag gtagcgggcg
     8941 cacgactcga ggccggcctt ggccacaccc atccagtcgt aggccggcca cgccaccgtc
     9001 gcgtcgaagg tcagcccgac gaccgagccg cccttgctca tcagcggcag ggccgccgtg
     9061 gtgagcgagg ccagcgagaa cgccgacacc tgcacggcct gggccacgtc ggcccacgga
     9121 gtggtgagca gcttgccgcc gagggcggtc tcggggttgg caaacgcgat cgagtgcacg
     9181 accccgtcga gcccgtcgac gtgctcgcgc agcttgtcgg cgagccccgc caggtgctcg
     9241 gggtcggtga cgtcgagctc gatcaccggc ggctcggtcg gcagccgctt ggcgatcgtg
     9301 cgggtgatcg tcaacgcgcg tccgaagttg gagatcacca cctcggcgcc ctgctcctgg
     9361 gccacccggg cgaccgcgaa gccgatcgag gtgtccaggg tgacaccggc caccaggatg
     9421 cgcttgccgt cgaggatgcc gcccactgct ccgttgtctc cttcgtcgtc gtgtgtcggc
     9481 cgcccacccg gtggccacgt agcggacgga ccgtcagtgg cccatgccga gccctccgtc
     9541 gaccgggatg accgccccgg tgatgtaggc ggcctcgtcg ctggccagga aatgcaccac
     9601 ccgggcgacc tcgtcactgc tggcgaagcg cccgagcggg atctggccct ggtaggtggt
     9661 gcgcaacgtg tcgggcaggg ccgcggtcat gtcggtctcc acgaagccgg gcgcgacgac
     9721 gttggcggtg atggcccgcg agcccagctc ccgggccagt gatcgggcca ggccgaccag
     9781 cccggccttg gacgcggcgt agttgacctg accggccgat cccagcatgc cgacgaccga
     9841 cgagatgaag atcatccggc cccggcgcag ccgcaccatc cccttggtcg cgcgctgggc
     9901 cacccgccag gcacctgcga ggttggtgtc gaccaccgcg gtgaagtcgt cgtcggccat
     9961 ccgcatgacc agctggtcac gggtgatccc ggcgttggcg accaggatct cgaccggccc
    10021 gtgagcggcc tcgaccgacg cgaacgccgc gtcgagcgag gacgggtctg tgacgtcggc
    10081 tgcgaccgcg tgcagcccct ccggcgcgct gcccgaccgg gaggtcacgg ccacccggtc
    10141 accatgcgcg gcgaaggcct gcgcgatggc cagcccgatg ccccggttgc cgccggtcac
    10201 cagcaccgag cgtccctcga cggtcgtcat ctccacctcg tagcagccac ggtgcgatcg
    10261 gcctcggccg agcaggcacc caggtggtgc cgggggcacg ctatcgggtg ccggtgccta
    10321 gctgtcttcg aggcggaagc cgaccttcat cccgacctgg aagtggtcga cctggccgtc
    10381 gacgacgtgc ccacggatct cgccgacttg gaaccagtcg atgtggcgca aggtctgggc
    10441 cgctcgagcc agaccgttgc gcacggccgc atcgatcccc tcgggcgagg tacccacgat
    10501 ctcggtgacg cggtatgtcc ggttggccat ggctcctcct gtgactgggt gcgacctggc
    10561 ctcgccgcgc cggcgggcct gacctaccgt ggagacgtga cgagtcccga cggcgagcgt
    10621 ggcaagccga ccgtctaccg gatcaccgac gcccgcaagg ggatcaccga ggacgtcaac
    10681 gagcggcagc gtcgctacct catctcgatg ggcatccgca cggtctgctt cgtgctcgcc
    10741 ctggtgacct ccggctggct gaggtgggcg atgatcatcg ccgcactgct cctgccctac
    10801 atcgcggtga tcatcgccaa cgccggccgg gagcaggcgc cggcgccgct ggacaccgat
    10861 ctgcacgagg acccgccgag tctgcccggc ggccgcggcg aggacccgcc aaccgcgtga
    10921 accgctccgg cgcgacagtc ccgcgcggca ggcggccatg ccataatttc agcgcggcgt
    10981 ttcgttcccc cgtcgaagcg ccggacgcga tgccgggcag cttcccccgt ggctgcccgg
    11041 catcgcctct ttgagaggct gacgtcgtga ccgaccagcc gcccgcgccc gccgaatcga
    11101 tccctcccac ctgctcgcgg cagggctgtc gagccccggc cacctgggtg ttggtgtgga
    11161 acaaccccaa ggtgcacacc ccggagcggc gcaagacctg gagcgcctgc gacgagcacc
    11221 gggagtacct gacctactac ctcgaccggc gcacgttttt gatcgaggtc gtggcccgcg
    11281 acgactggga ggcgcggacc tcaccgcagg gcccgccgag ctgacgggct agccgccgat
    11341 ggccgacatc ggtcgcgacg gctgcaggaa gttggggtcg tcgatgccgt gcccggcccg
    11401 cttggcccac atggtggact tgaagatctc ggccagctcg tcgtcgctgg cgcccgcgcg
    11461 catcggggcg cgcaggtcgg actcgccgcg ggcgaacagg cagttgcgca gctggccgtc
    11521 ggcggtgagc cgcacccggt cgcaggcacc gcagaacggg cgggtcacgc tgccgatgat
    11581 gccgaccttg ccggggccac cgtcgacgat ccaggtctcg gcgggcgcgc ttccgcgcac
    11641 cgactcgccc tccggggtga gctcgaagtg ctgggagagc agctcgagca cctctgtcgc
    11701 cgtgaccatg gtcgagcggt cccagccgtg ctgggggtcg agcggcatct gctcgatgaa
    11761 gcgcagctgg taaccctgct cgaggcacca gcgcagcagc tcgggggcgc tggcgtagtt
    11821 gatctcgcgc atcaccaccg cgttgacctt gacgggcgcg agcccgctgc gggccgcggc
    11881 cgccagcccg gccagcacgt cgtcgagccg gtcgcgccgg gccagcgcgg cgaactgctc
    11941 ccggtcgagc gtgtcgagcg agacgttgat ccggtcgagg ccggcgtcgg ccagcggctg
    12001 ggccaaccgg tcgagcccga tcccgttggt ggtcagcgcc atggacggac gcggctcgag
    12061 ctcgccgatg cgccggacga tgtcgggcag cccacggcgc agcaacggct cgccaccggt
    12121 gaaccgcacc gaggtgatcc ccagctctac ggcgatgcgc accagacgca cgatctcgtc
    12181 atcggtgagc agctggggct tgggcagcca ctgcaggccc tcggcaggca tgcagtagac
    12241 acagcgaagg ttgcaccggt cggtgagcga cacccgcagg tcgcggccca cccggccgaa
    12301 ccggtcgaca agctgtcccg ccggcgcgct gagcatgccg actcccttcc ccttggtggc
    12361 cctaccctac gtcgccacct cgcgcgggct ctagacacga cgcgcaggta ccgtcgaggc
    12421 gtgcatcgca ccctgctgac ccgacgctgg ctcggcttgc tcgtgctgtg cctgctgctc
    12481 gccacagtgt gcgtgcggct cgggttctgg cagctgcacc ggttcgagga ccaccgagcc
    12541 gccaaccagc gcgtgaccca gcagatctcg gccgacccgg tgccgctcca gaccctcctg
    12601 ccagtcggcc gctcggtggg cccagcgcag gagtggcgac gggtgacggc cagcggcgag
    12661 tacgacgtga cccggcagct gctggtccgt cagcgccccc tcaacggggc caacggctac
    12721 cacgtgctca ccccgctcgt gcttgccgac gggagcgcgc tcatcatcga tcgcgggtgg
    12781 gtgccggctg gtgcgtcggc gacgacctcg ccggcggtgc ccgctccccc ggacgggcag
    12841 gtcacggtca ccgcccggct gcggcccagc gagaccggcc ccctgcagca ggccggcctg
    12901 ccgcctggcc agatccgtcg gatcgccgtg gccgagatcg ccgccgacct gccctacccg
    12961 gtggcggacg gctacgccga gctcgtcggc cagacgcccg cccccgccca ggagctgtcc
    13021 tcgctcccgc tggagcagaa gtcgaccgcc ctcaacctgg cctacgccgt gcagtggtgg
    13081 ctgttcgcgg ccatcatcgt cgggggttgg tacttcctcg gacgacgaga ggcgctcgac
    13141 gagcaggccc gccggggggc cgacctcgac ccagcgcaga ccgcggtcgg gggctagccc
    13201 gagggggcac ggcatggcgt cacggatcga ggactacgcc ctcgtcggcg acatggagtc
    13261 ggccgcgctg gtcggacgca acgggtcgat cgactggctg tgcgtgccgc gcttcgactc
    13321 cccggcgatc ttcgccgctc tggtcgggag cgccgacaac gggcactgga tcatcgcccc
    13381 ggtgggcgcc ggtgacgcga cacggcgacg ctaccgacct gacacgctga tcctcgagca
    13441 ggagtgggac accgccgacg gccggctgcg cctgctcgac ttcatgccgg ctcgggacaa
    13501 ggcacccaac ctcgtgcgca tcgtgacctg cctgtctggc gaggtggcgg tgcgcagcgt
    13561 gctgcggctg cggttcgact acgggcgcgt cctgccgtgg gtcacgcatc gggacgggca
    13621 tctcagcgcg gtcgccgggc ccgacgccgt caacatcacg agcaccgtcc ccttgcacgg
    13681 gcgagacttc gcgacccaat cggaggtcac actccaggcg ggccagagcg cctcgttcgt
    13741 gctgacctgg cacccgtcct acgagccgga gccctccccc gtcgagccgg acgaggcgct
    13801 cgcgcagacc gagcagttct ggaaggactg ggtcgcccgg tcaacctacg aggggcctta
    13861 ccgggaggcc gtcgtacgct ccctgatcac cctgaaggcg ctcacgttcg agccgaccgg
    13921 tgggctcgtc gccgccccga cgacgtcgtt gcccgaggac ctcggcggaa cgcgcaactg
    13981 ggactaccgg ttctgctggt tgcgcgacgc ctcgctgacg ctgcgggcgc tgatcgcgac
    14041 cggtcacacc gaagaagcgg aggcgtggcg aggctggctg ctgcgtgcga tcgccggtga
    14101 cccggccgac ctgcagatca tgtacgggct ggcgggtgag cgccggctgc cggaatggag
    14161 cccggagtgg ctggtcggct atgaggggtc cacgcccgtc cggatcggca acgacgcggc
    14221 tgtgcaacgc cagctcgacg tctacggcga ggtgctcgac acgcttgacc ttgccggggc
    14281 ggccggcttg tccagcgacg cggcgacctg ggccatccag gtcgcgctgc tcgactggct
    14341 ggagcagcac tggcaggagc cggacgaggg gctgtgggag gtccgggggc cgcggcggca
    14401 gttcgtgcac tccaaggtgc tggcctgggt cgccttcgac cgggcggcca ggatggctca
    14461 cgccctcggc cacgctcagc acgccgcccg ctggtcgcgc gtgcgcgacg agatccacga
    14521 ggaggtgtgc cagaaggggt acgacgccga tcggggcacg ttcacccagt actacggctc
    14581 cgcgggcctg gacgcggcca cactgctgat cccgatgctg gggttcctgc ccggcgacga
    14641 cccccgagtg ctgggcacca tcgccgccgt cgaggcgaca ctggccgtcg acgggttcgt
    14701 cctgcgctat gacacgcacg gttcggtgga cgggctgccc gggcatgaag gggcgttcct
    14761 ggcgtgcagc ttctggctgg ccaccgcgct gcaccagtcg gggcgcaccg cggaggccac
    14821 cgcgttgttc gagcggctgc tgggcctgtg caacgacgtc gggctgctgt cggaggagta
    14881 cgacccgcgc tatcagcgtc tggtgggcaa cttcccgcag gccttcagcc acatcggtct
    14941 gctcaacacc gcgatcacgc tggcgcaccc gcccgggcag gcgccgttcc tggtccggca
    15001 ccagcggggc acccgcaagg gcgacccccg cccgcgctga gcacggaggg tcgacttcgc
    15061 cgggtctgcc ctctgacggc aggatggccc catggatctc ggactcgacg gccaggtctt
    15121 catcgtcacc gggggcaccc gggggctcgg ccgtgccacc gtggacgtgc tggtcgccga
    15181 aggtgcccgc gtggtggtct gctcgcgcga cggcgccgct gtcgccaaga ccagcgccga
    15241 gcacggcggc agtgaccggg tgctggggat cgccggcgac ctggccgaag ccggcacgga
    15301 gacccggctg gtcgccgccg ccatcgctcg ttacggacgc ctcgacggcg cactggtcag
    15361 cgtcgggggg ccgccggcgg gcccgagcca tgcgctcacc gacgaggtct ggcgctcggc
    15421 gttcgagtcg gtgttcctcg gagcgctgcg cgtggcccgt gcggtggtca aggccctgcc
    15481 ggccgagggc ggctcgatcg cgttcgtgct ctcgtcgtcg gtgcgcaacc cgatcgccgg
    15541 gctggccgcc tccaacgggc tgcgccccgg cctggcgatg gtagccaaga ccatggccga
    15601 cgagctcggc gcgcgcaacg tgcgggtcaa cgggctgctg cccggccgga tcgccaccga
    15661 ccggctgcgt gagctcgacg aacgcaccgg ccgcccgcag gcggcccgcc gcgacagtga
    15721 ggcgcgcatc ccgctcggcc gctacgggca gcccgaggag ttcgggcggg tggccgcgtt
    15781 cgtgctctcg ccggcggcct cctacctgac cggtgccatg gtgccggtcg acggcggcgc
    15841 gatccgcgcg ctgtgagtct tagcccaggc ggccgaagat gacgtcgggc ggcgcctccg
    15901 ccggaggcgg cccctgcccg cgatcgaact gggtgcggta gagctcggcg tagagcccgc
    15961 ccgcggccag caggtcggcg tggcggccgc gctcgacgat ccgtccgtcg tcgacgacca
    16021 ggatctgctc ggcgtgacga accgtggaca gccggtgggc gatcacgatc gaggtgcgcc
    16081 cggccagagc ggtggccagg gcctgctgga ccgccagctc gctctcggag tcgaggtggg
    16141 cggtcgcctc gtcgagcacc acgatcgacg gggccttgag cagcagccgg gcgattgcca
    16201 gccgctgctt ctcgccgccg gacagccggt agccccggtc gccgacgacc gtgtcgagcc
    16261 cgtcgcgcag cgaggcgacc aggtcccaga tctgcgccga gcggcaggcc tcgaccagct
    16321 ccgcctcgct ggcctcggga cgggcgaaca gcaggttgtt gcggatggtg tcgtggaaca
    16381 tgtgcgcgtc ctgggtcacc accccgaccg tgcggtgcag cgactcctgg gtgacgtcgc
    16441 gcacgtcgtg ccccccgatg cggaccgcac ccgaggtcac gtcgtacaac cgggagacca
    16501 gcgcggtgat cgtggtcttg ccggcaccgg agggccccac cagggcgacc agctcgccgg
    16561 gggcggcgac gaacgacacg tcgtgcagca cctgcgtggg cggggcggag tcgagcaccg
    16621 ccaccgactc cagcgaggcc agcgagacct cgtcggccgc cgggtagcgg aacgcgacgt
    16681 ggtcgagctc gaccgaggcc gcgccgggcg gcagtgtcgt ggcaccgggt cggtcggcca
    16741 ccatcggcgg caggtcgagc acctcgaaga cgcggtcgaa gctgaccagc gccgtcatca
    16801 cgtcgacgtg cacgttggac aacgcggtga gcggaccgta gaggcgggtc atcagcgccg
    16861 tcagggcgac cagggtgccc accgccagct cgccgtccac cgccagcagc ccgccgatgc
    16921 catagaccag cgccgtcgcg agcgccgcga ccagggtcag cgccgtgaag aacacccggc
    16981 tgtacatcgc ctggatcacc ccgatgtcgc ggacccgggc cgccttgtcg gtgaacgccg
    17041 cctgctcgtc gggcagccgc ccgaagatct tgaccagcag ggcgcccgag acgttgaacc
    17101 gttcggtcat cgtctggctc atcgcggcat tgagctgcat cgcctcgcgg gtgatcaccg
    17161 cgaggcggcg tccgacgagc ttggcaggga tgacgaagac cggcagcagg agcagcgagg
    17221 cgagcgtgac ctgccacgac aggatgagca tcgccacgag cacgaggacg agaccgacaa
    17281 cgttggacac gaccgaggac agcgtcgacg tgagggcctg ctgggcgccg atcacatcgc
    17341 tgttgagccg gctcacca
//
LOCUS       DATIBB010000096         4850 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_1759767, whole genome shotgun sequence.
ACCESSION   DATIBB010000096 DATIBB010000000
VERSION     DATIBB010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 4850)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4850)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4850
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1759767"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1641
                     /locus_tag="VLC50_03585"
     CDS             <1..1641
                     /locus_tag="VLC50_03585"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase C-terminal domain-containing
                     protein"
                     /protein_id="HSF26580.1"
                     /translation="VAVAGERIALVGDASHCLGAGTRVLDCTGRFVLPGLVEPHFHVG
                     GSQCSIERLAEVLVPAGTVALGTCFYEAAIVAGREAVEEQLRRAEATALDVLFSPFVA
                     SLGQGDLGASRTSMADLLELIDHPACIELREWNLSSHVPELREVYLRARARGAVIGGH
                     LEGLAGPALSASAALGARSDHETSSAAEALEKVRAGVTVQIREGSGARDLVAVTRAIT
                     EHGADPARFALTVDQQELWSIAERGHLDDKLRMVVGQGVGPIDAVRMATINAARSLGV
                     DDRYGAVAPGFLASVLVVDDLRRFTVEQVVARGRLVAEHGRYLPASDPAPYPAPWSQT
                     VHVPGRLRADDFRLRPTSRRLRVIGVTPGSLLAEELEEDVELRGGMPLAEHGLAAMAV
                     LDRHEGSGRVGRGLVRGLGLRAGAVAATVTPGMVNLMVIGVDAADMALAANRLVELQG
                     GIVVVVDGEVRAEVALPLFGLVSADPLEQTLAACRRVAAAVRDDLGAPDPDVLSNAAF
                     VTLPRSIPRLKLCDHGLVRVSREGSRERVSFEVDDLAV"
     gene            1835..2908
                     /locus_tag="VLC50_03590"
     CDS             1835..2908
                     /locus_tag="VLC50_03590"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSF26581.1"
                     /translation="MTLRVPAAAETTSGSPLRELVARAPRDVVRLFIGVLVLAGIFSV
                     TYQHAFPTITNLENMSRQAGILLVIAIGQAFPLLIGGFDLSIASTMGLAGTVSALVML
                     EHGVVAGVFVGLVAATLAGLANGLLIGVLKVDPFIATLGSLTLVSGLANHLSDGASVT
                     GLPTSFANVGASDWGPIPSATGIGIVVLVLAWLVLARSRMGLYTYAIGGSRESCVVAG
                     IPVARYEVAAYTISGLLAGVGGIMVASRVGVGQADVGQGYALLSIATAVIGGVAIGGG
                     VGRLTGVVLGVALLTVITTGMNIAGISSFTQQMLQGAVVVAAVLLNRANKAGRKRRRG
                     SPDATSGRSGVPPPQPVPEPSEG"
     gene            2905..4002
                     /locus_tag="VLC50_03595"
     CDS             2905..4002
                     /locus_tag="VLC50_03595"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HSF26582.1"
                     /translation="MTMRQRITAVASLVAAGALLAACGGSTPASTTSQSAAPGTTAAK
                     DLVRSAADGKALDCQTQLTSEDTIGYKVPKAKEKFRVTLMEVSLAAYYYQGIKKGAED
                     AAREAGVDLEVLAGQGYASPQQQLEQAQAALQRGTDAIILAPSDINGSAPIVKLAQDQ
                     GVPVVNVSTFVAAPGAYHVLQDDAALGAAAADRVAEALGAEGGEGIIIAGPANADWSK
                     ARTEAFQARVAEKHPQLKVAAAPTQLVDPVAGLKSFEDAVQAHPNIKWIFNVHVAILP
                     PASIPSPYKGNVTYVATGYEPDAVPALKDGSLDSTFGIEPIATGKVGVGTAVNVLNGT
                     EQPHSQCLPVSLFTKDDVGSPAADAQLIPAS"
     gene            4005..>4850
                     /locus_tag="VLC50_03600"
     CDS             4005..>4850
                     /locus_tag="VLC50_03600"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HSF26583.1"
                     /translation="MTSPAVGSDLAVSLRHICKGYPGVQALDAVSLDVRFGEVHAIVG
                     ENGAGKSTLLKILSGLVEPDCGTVQVSGTILKPHTTIAARRLGVRVVPQDVLVVQGLI
                     AGRNVTLGQERWNAHRGRLSGPERAAVGEAMTLAGLASPATVRSGRMSVPEQRLLQIA
                     AGLYGSGKVLVLDEPTAVVSEADSEHLLQRLNALREQGMAILYVSHRLTEVLTIADRV
                     TVLRDGRKVAENPRAEVTREGLIRHMTKVSASSTPTADGTAAPGGDAPASGSGSGRPH
                     ALTQAS"
BASE COUNT          673 a         1748 c         1738 g          691 t
ORIGIN      
        1 gtcgcggtcg ccggcgagcg gatcgcgctc gtcggcgacg cgtcgcactg cctcggcgca
       61 ggcacccgcg tgctcgactg caccgggcgg ttcgtgctgc ccggcctggt cgagccgcac
      121 ttccacgtcg gcggctcgca gtgcagcatc gagcggctgg ccgaggtgct ggtgccggcc
      181 ggcacggtgg cgctgggcac ctgcttctac gaggcggcga tcgtggccgg tcgcgaggcg
      241 gtcgaggagc agctgcgcag ggcggaggcc accgccctcg acgtgctgtt ctcgccgttc
      301 gtcgcctcgc tgggtcaggg tgacctgggc gccagccgca cctcgatggc cgacctgctc
      361 gagctcatcg accaccccgc ctgcatcgag ctgcgggagt ggaacctgtc gtctcacgtg
      421 cccgagctgc gcgaggtcta cctgcgcgcg cgggcccggg gcgcggtgat cggcgggcac
      481 ctggagggcc tggcggggcc ggcgctgtcg gcgtcggccg cgctgggcgc caggtcagac
      541 cacgagacga gcagcgcggc cgaggcgctg gagaaggtcc gggccggcgt gacggtgcag
      601 atccgcgagg gctctggggc gcgggacctg gttgccgtca ccagggcgat caccgagcac
      661 ggtgccgacc cggcgcggtt cgcgctcacc gtggaccagc aggagctgtg gtcgatcgcc
      721 gagcgcggtc acctcgacga caagctgcgc atggtcgtcg ggcagggcgt cgggccgatc
      781 gacgccgtac gcatggccac gatcaatgcg gcccgcagtc tcggcgtcga cgaccgctat
      841 ggcgcggtgg cgcccggctt cctcgcctcc gtgctggtcg tcgacgacct gcgccgcttc
      901 acggtggagc aggtggtggc gcgcggacgg ctcgtggccg agcacggtcg ctacctgccg
      961 gcgtccgacc ccgcgcccta cccggcgccc tggtcgcaga ccgtgcacgt gccggggcgg
     1021 ctgcgggccg acgacttccg gctccgcccg accagccggc ggttgcgggt catcggggtc
     1081 acgcccggct cgctgctcgc cgaggagctc gaggaggacg tcgagctgcg cggcgggatg
     1141 cccttggccg agcacgggct ggccgcgatg gcggtgctcg accggcacga gggatcaggc
     1201 cgggtgggcc gggggctggt gcgcgggctt ggcctgcggg ccggtgcggt ggccgcgacc
     1261 gtcacaccgg gcatggtcaa tctcatggtg atcggggtgg acgccgccga catggcgctg
     1321 gccgccaacc ggctcgtcga gctgcagggc ggcatcgtcg tcgtggtcga cggcgaggtc
     1381 cgggccgagg tggcgctgcc gttgttcggc ctggtctcgg cggacccgct cgagcagacg
     1441 ctggccgcgt gccggcgtgt cgccgccgcc gtgcgcgacg acctgggcgc tcccgacccc
     1501 gacgtgctgt ccaacgccgc gttcgtcacc ctgccccgca gcatcccgcg gctgaagctc
     1561 tgcgaccacg gactggtccg ggtcagccgc gagggatccc gcgagcgggt ctccttcgag
     1621 gtcgacgacc tcgccgtcta gcaacgtgcg acgcgcgacg gttcgaagcg acgatcgtta
     1681 acaaccgatt gcgtgatcga ccttgacaaa cgcgaagaaa cccgacgatg ctggactcac
     1741 aatcgattgt catcagaacg acaccgcggg ctcgcgccgt cgaaccgctg ggccttgacc
     1801 aagccgccga gccaggacca aggaggtagg ccgcatgacg ttgcgcgtgc cggccgcggc
     1861 cgagacgacg tctggctcgc cgctgcgtga gctggtggcc cgcgcccctc gcgacgtggt
     1921 gcgcctcttc atcggcgtcc tggtgctggc cgggatcttc tcggtcacct accagcacgc
     1981 attcccgacg atcaccaacc tcgagaacat gagccggcag gccgggatcc tgctggtcat
     2041 cgccatcggg caggccttcc cgttgctgat cggcgggttc gacctgtcca tcgccagcac
     2101 gatgggtctg gccggcaccg tctccgcgct ggtcatgctc gagcacggcg tggtggcggg
     2161 cgtcttcgtc ggactcgtcg cggcaactct ggccggcctg gccaacgggc tgctcatcgg
     2221 cgtgctcaag gtggacccgt tcatcgccac cctcggctcg ttgacgctcg tgtccggcct
     2281 ggccaaccac ctcagcgacg gcgcgtccgt caccgggctg cccacgtcgt tcgccaacgt
     2341 gggcgccagc gactggggcc cgatcccgtc ggcgaccggc atcgggatcg tcgtactggt
     2401 gctggcctgg ctcgtgctgg cccgcagccg gatgggcctc tacacctacg cgatcggcgg
     2461 cagccgcgag tcctgcgtcg tggctggcat cccggtggcg cgctacgagg tggcggccta
     2521 cacgatcagc gggctgctcg caggcgtcgg cgggatcatg gtcgcctccc gggtgggtgt
     2581 cgggcaggcc gacgtgggcc agggctacgc cctgctgtcc atcgctaccg cggtcatcgg
     2641 cggtgtcgcg atcggcggtg gcgtcgggcg gctgaccggc gtcgtactcg gtgtcgcgct
     2701 gctcacggtg atcaccaccg gcatgaacat cgcgggcatc tcgtcgttca cccagcagat
     2761 gctccagggc gcagtcgtcg tggccgcggt cctgctcaac cgcgccaaca aggccggtcg
     2821 caagcgccgg cgcggctcac ctgacgcgac gtcgggtcgg tcaggcgttc caccacctca
     2881 acccgtccca gaaccatcgg aaggatgacc atgcgtcaac gcatcaccgc tgtggccagc
     2941 ttggtcgcag ccggagccct cctcgccgcg tgcggaggct ccaccccagc gagcaccacc
     3001 agccagagcg ccgcgccagg caccaccgcg gccaaggacc tggtgcgcag cgccgcagac
     3061 ggcaaggccc tcgactgcca gacacagctg acctccgagg acaccattgg ctacaaggtg
     3121 cccaaggcca aggagaagtt ccgcgtcacg ctgatggaag tgagcctggc ggcgtattac
     3181 taccagggca tcaagaaggg cgccgaggac gcggccaggg aggccggcgt cgacctcgag
     3241 gtgctcgcgg gccagggcta cgcgtcgccg cagcagcagc tcgagcaggc ccaggcggcg
     3301 ctgcagcgcg gcaccgacgc gatcatcctg gcgccgtcgg acatcaacgg ctcggccccg
     3361 atcgtcaagc tggcccagga ccagggcgtg ccggtcgtca acgtgtcgac gttcgtggcc
     3421 gcccccgggg cctaccacgt gctgcaggac gacgcggcgc tcggagcagc ggccgcggac
     3481 agggttgccg aggcgctcgg cgccgagggt ggcgagggca tcatcatcgc cggcccggcc
     3541 aacgccgact ggtccaaggc ccgcaccgag gcgttccagg cccgcgtcgc cgagaagcac
     3601 ccgcagctca aggtggccgc cgccccgacc cagctggtcg acccggtcgc cggcctgaag
     3661 tcgttcgagg acgccgtgca ggcccacccc aacatcaagt ggatcttcaa cgtccacgtg
     3721 gcgatcctgc cgccggcctc gatccccagc ccctacaagg gcaacgtcac ctacgtggcc
     3781 accggctacg agcccgacgc ggtgccggcg ctgaaggacg gcagcctcga ctcgacgttc
     3841 ggcatcgagc cgatcgccac gggcaaggtc ggcgtcggca cggcggtgaa cgtcctcaac
     3901 ggcacggagc agccgcacag ccagtgcctg ccggtgtcgc tgttcaccaa ggacgacgtg
     3961 ggcagcccgg ccgccgacgc gcagctgatc ccggcctcct gaccatgacc agtcctgcag
     4021 tcgggtccga cctggcggtg tcgcttcggc acatctgcaa gggctacccg ggcgtccagg
     4081 ccctggacgc ggtgtcgctc gacgtccgct tcggtgaggt ccatgccatc gtcggcgaga
     4141 acggcgccgg caagtcgacc ctgctgaaga ttctctccgg actggtcgag cccgactgcg
     4201 ggacggttca ggtctccggc acgatcctca aaccccacac gacgatcgcg gcccgccggc
     4261 tcggcgtacg cgtcgtcccg caggacgtgc tcgtcgtaca ggggctcatc gctggccgca
     4321 acgtcacgct cggccaggaa cgctggaacg cccaccgcgg ccggctctcg ggacccgagc
     4381 gggccgcggt gggcgaggcg atgacgctgg ccggtctggc cagcccggcc accgtgaggt
     4441 ccggccggat gtcggtgccc gagcagcggc tcctgcagat cgcggctggg ctctatggct
     4501 cgggcaaggt gctggtgctc gacgagccca ccgcggtggt ctccgaggcc gactccgagc
     4561 acctgctgca acgcctgaat gccttgcgcg agcaggggat ggcgatcctc tacgtctccc
     4621 accggctcac cgaggtgctc accatcgccg accgggtgac cgtgctgcgc gacgggcgca
     4681 aggtcgccga gaaccctcgc gccgaggtca cccgcgaggg gctgatccgg cacatgacca
     4741 aggtcagcgc cagctcgaca cccaccgcgg acggtacggc ggcccccggc ggcgacgcgc
     4801 ccgccagcgg ctcgggctcg ggccgccctc acgcgctcac gcaggcctcc
//
LOCUS       DATIBB010000097         6763 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_51676, whole genome shotgun sequence.
ACCESSION   DATIBB010000097 DATIBB010000000
VERSION     DATIBB010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 6763)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6763)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6763
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51676"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..793)
                     /gene="sufB"
                     /locus_tag="VLC50_03605"
     CDS             complement(<1..793)
                     /gene="sufB"
                     /locus_tag="VLC50_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003976894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HSF26584.1"
                     /translation="MTSTTESLHPELAGLGTYEYGWADSDAAGAAAQRGLSEDVVRDI
                     SAKKNEPEWMLKSRLKALRLFQKKPMPSWGSDLTGIDFDNIKYFVRSTEKQAQSWDDL
                     PADIRNTYDRLGIPEAEKQALVGGVAAQYESEVVYHKIRDDLEEQGVIFVDTDTGLRD
                     YPELFQEYFASVIPLGDNKFAALNSATWSGGSFVYVPKGVHVEIPLQAYFRINTENMG
                     QFERTLIIADEGSYVHYVEGCTAPVYSSDSLHSAVVEIVVKKDARV"
     gene            complement(790..1527)
                     /locus_tag="VLC50_03610"
     CDS             complement(790..1527)
                     /locus_tag="VLC50_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017557474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HSF26585.1"
                     /translation="MLIETTPAAPAEDRTRDRVARSLRDHGASTVASLAERLDLTAAA
                     VRRHLDGLLEAGLVEARQARSYGSRGRGRPPRVFALTDAGRDQFEHTYDDLATDALRF
                     LADTAGDEAVLEFARARVAELEARYAARFAATPPADRPRLLAEVLRTGGYAAAVGGAP
                     RGGGEQLFQHHCPVAHVAKQFPQLCEAETEMFSRLLGTHVQRLATIAHGDGVCTTNIP
                     SSTPHDPTHPSTTHPSTTHPTRGGAHR"
     gene            1654..2577
                     /locus_tag="VLC50_03615"
     CDS             1654..2577
                     /locus_tag="VLC50_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014141847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSF26586.1"
                     /translation="MPAPAVEVVDLVKRYGDLVAVDGLGFAVATGTITAVLGPNGAGK
                     TSTIECAEGYRRPDAGVVRVLGLDPRADGNRLRPRIGVMLQQGGIYPAVHAVEFLRHV
                     ASLYARPLDVGMLVDRLGLGSCGRTPFRRLSGGQQQRLSLALAIVGRPELVFLDEPTA
                     GLDPQARHATWELVGALRDDGVTVVLTTHYMEEAERLADHVVVIDHGRVVAAGSPAAL
                     TAADGDEGHVRFSGPARLDLDALRVALPRSASVVEEAPGSYRVRGEVDPQLLATLATW
                     CAANGVLLDDLHVARRSLEDVFLDLTGRELR"
     gene            2574..3350
                     /locus_tag="VLC50_03620"
     CDS             2574..3350
                     /locus_tag="VLC50_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016642180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSF26587.1"
                     /translation="MSAPAQPTFAPRPGAAPMPRMVARQAWLELTLMLRNGEQLVLAL
                     VIPVLVLVGVTLVDAVDLGPGRRVDIITPGVFALAVMSTAFTGQAIATGFDRRYGVLK
                     RLGATPLPRWGLIAAKTACVLVVELGQLLVLALVATRLGWHPRGSVLDVAALLLVGTA
                     AFSGLGLLMAGTLRAEATLAGANLVWLLLFVGGGIVVPLDQMPEGVAAGLAYLPSAAL
                     TEGLRDVLQHGASLPWPHLGVLLVWAGVGVGLASRTFRWE"
     gene            3341..4414
                     /locus_tag="VLC50_03625"
     CDS             3341..4414
                     /locus_tag="VLC50_03625"
                     /inference="COORDINATES: protein motif:HMM:NF014665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0016653 - oxidoreductase activity, acting
                     on NAD(P)H, heme protein as acceptor [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HSF26588.1"
                     /translation="MGVTTGAPLPSRLVRRLRARPSPRTLRGFAIASLAANLLLVVSG
                     GVVRLTASGLGCPTFPRCTEQSLVPTAEMGVHGLIEFGNRMLTFVLAAVAVATLVVAW
                     RVRPARRDVRRLALLLAFSIPVQALIGGVVVLTELNPWTVMLHFLASLVLVGLATVLM
                     QRAIEGDEPAQPVAGRPLRRMALLTLLVAAATVYAGAVVTGTGPHAGDRDARRTGLDL
                     LWVTQLHADLVFLLVGLSVGLLVLAHAVHAPRRAIRAAWVLVAVEAAQAVIGYTQYFT
                     GLPIVLVAVHMLGSGLLVAAATDAWLATRARGPVAGSTLGGADERGDRGGLVEVTHQV
                     VDVAGPSGGVSADEPHPTGPDRH"
     gene            complement(4765..5685)
                     /locus_tag="VLC50_03630"
     CDS             complement(4765..5685)
                     /locus_tag="VLC50_03630"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018382708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HSF26589.1"
                     /translation="MQPPAPPASRRGLRATLSAYVALTKPRIIELLLVTTVPTMLLAE
                     GGLPSLWLVLATLVGGSLAAGSAHTLNCYIDRDIDVIMHRTGRRPLVTGEVTPRAALT
                     FGLITGAASVVWLVLTVNALSAVLALLAIVIYVVGYTMLLKRRTAQNIVWGGTAGCMP
                     VLIGWAAVTNRLDWAPVVLFMVIFFWTPPHYWPLSMRFRDDYAAAGVPMLPVVASPAA
                     VARQVVLYSWAMVLTSLLLWPVAGTTLVYPLAAAGVGAVFLLEAHRLQARARRGLTGG
                     PLGAMRLFHWSITYLTLLFVAVAVDPLLPF"
     gene            5919..>6763
                     /locus_tag="VLC50_03635"
     CDS             5919..>6763
                     /locus_tag="VLC50_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020384820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HSF26590.1"
                     /translation="MSDPGTAASPLQWTELDRRAVDTARALAMDAVQKAGNGHPGTAM
                     SLAPLAYLLFQKVMRHDPTDPDWVARDRFVLSAGHSSLTLYTQLFYAGLLELDDLAQL
                     RTWGSLTPGHPEHGHTAGVETTTGPLGQGIGNAVGMAMAARRERGLLDPEAPAGQSVF
                     DHTIWCITSDGDIQEGVSSEASSLAGTQRLGNLVVIYDCNKISIEDDTDIALSEDTAA
                     RYRAYGWHVQEVDWTHDGERYVEDVQALHAALLAAKAERDRPSFIVLRTIIAWPAPNA
                     QNTGKA"
BASE COUNT          875 a         2480 c         2461 g          947 t
ORIGIN      
        1 ggactcgggc gtccttcttc accacgatct ccacgaccgc gctgtgcagc gagtcggagg
       61 agtagaccgg cgcggtgcag ccctcgacgt agtgcacgta ggaaccctcg tcggcgatga
      121 tcagggtgcg ctcgaactgg cccatgttct cggtgttgat gcggaagtag gcctgcagcg
      181 ggatctcgac gtgcacgccc ttggggacgt agacgaacga gccacccgac caggtcgcgc
      241 tgttgagcgc cgcgaacttg ttgtccccga gcgggatcac cgaggcgaag tactcctgga
      301 agagctcggg gtagtcgcgc agcccggtgt cggtgtcgac gaagatgacg ccctgctcct
      361 cgaggtcgtc acggatcttg tgatagacga cctcggactc gtactgcgcc gcgacccctc
      421 cgacgagcgc ctgcttctcg gcctcgggga tgcccagccg gtcgtaggtg ttgcggatgt
      481 cggccggcag gtcgtcccag ctctgggcct gcttctcggt ggagcgcacg aagtacttga
      541 tgttgtcgaa gtcgatgccg gtgaggtcgg agccccagga cggcatcggc ttcttctgga
      601 acagccgcag cgccttcagc cgcgacttca gcatccattc gggctcgttc ttcttggccg
      661 agatgtcgcg tacgacgtcc tcggacagcc cgcgctgggc ggcggcaccg gcggcgtcgg
      721 agtcggccca gccgtattcg taggtgccca gcccggcaag ctcgggatgc agactctctg
      781 tggtgctggt catcggtgcg cccctccgcg tgtcgggtgt gtcgtgctcg ggtgtgtcgt
      841 gctcgggtgt gtcgggtcgt gcggggtcga gctggggatg ttggtcgtgc agacgccgtc
      901 gccgtgggcg atcgtcgcca gccgctggac gtgggtgccc agcagccggg agaacatctc
      961 ggtctccgcc tcgcacagct gcgggaactg cttggcgacg tgggcgaccg ggcagtggtg
     1021 ctggaacagc tgctcgcccc ctccgcgggg cgccccgccc accgcggcgg cgtagccgcc
     1081 ggtgcgcagc acctcggcca gcagtcgcgg ccggtcggcc ggcggggtgg ccgcgaaccg
     1141 cgcggcgtag cgggcctcga gctctgccac gcgggcgcgg gcgaactcga ggaccgcctc
     1201 gtcaccggcg gtgtcggcga ggaagcgcag cgcgtcggtg gccaggtcgt cgtaggtgtg
     1261 ctcgaactgg tcgcgccccg cgtcggtcag cgcgaacacc cggggtgggc gcccgcgacc
     1321 gcgtgagccg taggaccggg cctggcgagc ctcgaccagg ccggcctcga gcagcccgtc
     1381 gaggtggcgg cggaccgccg cggcagtgag gtcgagccgc tcggcgagag aggcgaccgt
     1441 cgacgcgccg tggtcgcgga gggagcgcgc cacgcggtcg cgggtgcggt cctccgccgg
     1501 agcagccggg gtggtctcga ttaacacaac gccattgtcg cgtaaatggt cggccggctc
     1561 aacccggggg gtcccctgtg ccctcggaca catccgagcc ggctcgcgca cccgtcgaaa
     1621 atccggcccc agcaggctcc ctagactcgc agcgtgcccg ctcccgccgt cgaggtcgtc
     1681 gacctcgtca agcgctacgg cgacctcgtc gccgtcgacg gcttgggctt cgccgtcgcc
     1741 accggcacca tcaccgcggt gctcggcccc aacggcgccg gcaagacctc caccatcgag
     1801 tgcgcggagg gctaccgccg gcccgacgcc ggcgtcgtac gggtgctcgg cctggacccg
     1861 cgcgccgacg gcaaccggtt gcggccccgg atcggcgtga tgctccagca gggcgggatc
     1921 tacccggcgg tgcacgccgt cgagttcctc cgccacgtcg cctctctcta cgcccggccg
     1981 ctcgacgtcg gcatgctcgt cgaccggctc gggctcggct cgtgcgggcg cacgccgttt
     2041 cgccggctgt ccggcggaca gcagcagcgg ctgtcgctgg cgctggccat cgtcgggcgc
     2101 cccgagctgg tctttctcga cgagccgacg gccggcctcg acccgcaggc acgccatgcc
     2161 acctgggagc tggtcggcgc gctgcgtgac gacggcgtca ccgtggtcct cacgacccac
     2221 tacatggagg aggccgagcg gctggccgac cacgtcgtgg tgatcgacca cggccgggtc
     2281 gtcgcggcgg gctccccggc tgcgctcacg gccgccgacg gcgacgaggg ccatgtgcgc
     2341 ttctccgggc cggcccgcct cgacctggac gccttgcggg tcgcgctccc ccgctcggcg
     2401 agcgtcgtcg aggaggctcc tggcagctat cgcgtgcgcg gagaggtcga cccccagctg
     2461 ctcgccacgc tcgccacctg gtgcgccgcc aacggcgtgc tgctcgacga cctgcacgtg
     2521 gcgcggcgca gcctcgagga cgtcttcctc gacctgaccg ggcgggagct gcgatgagcg
     2581 cgccggccca gcccacgttc gccccccggc cgggcgcggc gccgatgccc cgcatggtgg
     2641 cccgccaggc gtggctagag ctgacgctga tgctgcgcaa cggcgagcag ctggtgctcg
     2701 ccctggtgat ccccgtgctg gtcctggtcg gggtgaccct cgtcgacgcg gtggacctcg
     2761 gcccggggcg ccgggtcgac atcatcaccc cgggcgtctt cgccctcgcg gtgatgtcga
     2821 ccgcgttcac cggccaggcg atcgccaccg gcttcgaccg ccgctatggc gtgctcaagc
     2881 ggctgggcgc cacgccgctg ccccggtggg gtctcatcgc ggccaagacg gcgtgcgtcc
     2941 tcgtggtcga gctgggccag ctgctcgtgc tcgcgctggt cgccacccgg ctcggctggc
     3001 acccgcgcgg gagcgtgctc gacgtggccg ccctgctcct ggtcggcacc gccgcgttca
     3061 gcgggctggg cctgctgatg gccggcacgc tgcgcgccga ggccactctg gccggcgcca
     3121 acctggtctg gctgctgctc ttcgtcggcg gcggcatcgt ggtgccgctc gaccagatgc
     3181 ccgagggtgt ggccgccgga ctggcctacc tgccctcggc cgcgctgacc gagggcctgc
     3241 gcgacgtgct ccagcacggc gcgagcctgc cctggcccca cctcggggtg ctgctcgtct
     3301 gggccggcgt cggcgtcggt ctggcctcca ggacgttccg atgggagtga cgaccggcgc
     3361 accgctgccc agccggctcg tccggcgcct gcgtgcgcga ccgtcgcccc gcacgctgcg
     3421 tggcttcgcg atcgcctcgc tggccgccaa cctgctgctg gtggtctccg gcggcgtggt
     3481 ccggctgacc gcctcgggcc tcgggtgccc gacgtttccc cgctgcaccg agcagtcgtt
     3541 ggtgccgacg gcggagatgg gcgtccacgg cctgatcgag ttcggcaacc ggatgctcac
     3601 gttcgtcctg gcggcggtgg cggttgccac cctcgtcgtg gcctggcggg tgcgtccggc
     3661 ccgccgcgac gtacggcggc tcgcgctgct gctcgccttc agcatcccgg tgcaggcgct
     3721 catcggaggg gtcgtcgtcc tcaccgagct caacccctgg acggtgatgc tgcacttcct
     3781 ggcctcgctc gtgctcgtgg ggctggccac ggtgctcatg cagcgggcca tcgagggcga
     3841 tgagccggcc cagccggtgg ccggccggcc gctgcgccgg atggcgctgc tcaccttgct
     3901 cgtcgcggcc gcgaccgtct acgccggcgc ggtcgttacc ggcaccggcc cgcacgccgg
     3961 cgaccgggac gcccgtcgta ccggcctgga cctgctctgg gtcacccagc tgcacgccga
     4021 cctggtcttc ctgctggtcg ggctctcggt cgggctgctc gtgctcgccc acgccgtgca
     4081 cgcgccgcgc cgggcgatcc gggccgcctg ggtcctggtc gcggtcgagg ccgcccaggc
     4141 cgtgatcggc tacacccagt acttcaccgg cctgccgatc gtcctggtgg ccgtgcacat
     4201 gctcggctcg gggctgctgg tcgccgcggc gaccgacgcc tggctggcca cccgcgcccg
     4261 cggaccggtg gcgggatcaa cgctcggcgg cgccgacgag cgcggcgacc gcggcggcct
     4321 cgtcgaggtc acgcaccagg tcgtcgacgt agccggcccc tccggaggcg tcagcgcaga
     4381 tgagccgcac ccgaccggcc ccgaccggca ctgaggtgac gcggaccgcg ggcccgcagg
     4441 tgcggcggat ccggcgagcc agcaccgcgg cccgccggga cggcgtcggg ccgggccgca
     4501 cgagcgagcg gccctggccg accagggcca ccgccgtgag cacgatcccg accaccgcga
     4561 tcggtgggct cgccgccagg cccaccaagg cgtcgaccag gccgaccacg agcagcgcgg
     4621 gccaccaggc gggtccgggc aggcgctgac ccgcgggcac gaccagctca cccggccgtg
     4681 tctcggccac cacccgccac ccggtcaggg ccagggcgac gcaggtggcg acgcccgcca
     4741 cgacggtgac cgtcaccgca gggctcagaa cggcaggagc gggtcgaccg ccaccgcgac
     4801 gaacagcagc gtcaggtagg tgatcgacca gtggaacaac cgcatcgcgc cgagcgggcc
     4861 gccggtcagg ccgcgccggg cccgcgcctg cagccggtgg gcctcgagca ggaacaccgc
     4921 gccgacgccg gcggcggcca gcgggtagac cagcgtggtg ccggccaccg gccagagcag
     4981 cagcgacgtc agcaccatcg cccagctgta gagcaccacc tgccgggcca cggcggccgg
     5041 cgaggccacc accggcagca tggggacccc ggccgcggcg tagtcgtcgc ggaagcgcat
     5101 cgacagcggc cagtagtgcg gcggcgtcca gaagaagatc accatgaaca gcacgaccgg
     5161 tgcccagtcg agccggttgg tcacggccgc ccagccgatg agcaccggca tgcagccggc
     5221 ggtgccgccc cagacgatgt tctgcgccgt acgccgcttg agcagcatgg tgtagccgac
     5281 gacgtagatg acgatcgcca gcagcgccag cacggccgag agggcgttga cggtcaggac
     5341 cagccacacg accgaggccg ccccggtgat caggccgaac gtcagcgccg cgcgaggcgt
     5401 cacctctccg gtgaccagcg gccgccgacc cgtccggtgc atgatcacgt caatgtcgcg
     5461 gtcgatgtag cagttgaggg tgtgcgcgct gccggccgcc aggctgccgc cgaccagcgt
     5521 ggcgagcacg agccacagcg acggcagccc gccctcggcc agcagcatcg tcgggaccgt
     5581 ggtgacgagc agaagctcga tgatccgtgg cttggtgagg gcgacgtagg ccgagagcgt
     5641 ggcgcgcagc cctcggcgag aagccggcgg ggcgggcggc tggatcgtcg tggtcgcacc
     5701 gcggcgcgga tagacggccg tcacgaacac ctcagggcag ctggtggggc gcctgctcgg
     5761 ctcgtctggc cgacgccgac gcgggtcacc tcactctaac gcccgccgcg tcgccgggat
     5821 cggctgggcg ggccttcccc gcggcccgaa cctgttggtc tgctcacagt aggctcgccg
     5881 ggtgggggat cgccgtcctc gagaggagtg cacgacaggt gagtgacccg gggacggcgg
     5941 catcgccgct gcagtggacc gagctcgacc ggcgggctgt cgacaccgcc cgcgccttgg
     6001 cgatggatgc cgtgcagaag gccggcaacg ggcatcccgg caccgcgatg agcctcgccc
     6061 cgctggccta cctgctgttt cagaaggtca tgcggcacga cccgacagat cccgactggg
     6121 tggctcggga ccgcttcgtc ctctcggccg ggcactccag cctcacgctc tacacccagc
     6181 tcttctacgc cggcctgctc gagctcgacg acctggcgca gctgcgcacc tggggcagcc
     6241 tgacccccgg gcaccccgag cacgggcaca ccgctggggt cgagacgacc accggaccgc
     6301 tgggccaggg catcggcaac gccgtgggca tggcgatggc cgctcgccgc gagcgggggc
     6361 tgctcgaccc cgaggcgccg gccgggcaga gcgtcttcga ccacacgatc tggtgcatca
     6421 cctccgacgg cgacatccag gagggcgtga gctcggaggc cagctcgctc gccggcaccc
     6481 agcggctggg caacctcgtc gtcatctatg actgcaacaa gatctccatc gaggacgaca
     6541 ccgacatcgc cctgtccgag gacaccgcgg cgcgctaccg ggcctacggc tggcacgtgc
     6601 aggaggtcga ctggacccac gacggcgagc gctacgtcga ggacgtgcag gcgctgcacg
     6661 ccgcgctgct cgcggccaag gcagagcgcg accggccgtc gttcatcgtg ctgcgcacga
     6721 tcatcgcgtg gccggcgccc aacgcccaga acaccggcaa ggc
//
LOCUS       DATIBB010000098        17668 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_315913, whole genome shotgun sequence.
ACCESSION   DATIBB010000098 DATIBB010000000
VERSION     DATIBB010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 17668)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17668)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17668
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_315913"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..888)
                     /locus_tag="VLC50_03640"
     CDS             complement(<1..888)
                     /locus_tag="VLC50_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011434478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA/Hda family protein"
                     /protein_id="HSF26591.1"
                     /translation="MSESAPAGEINLAEVWTRSLATLTGDDVAPNHRAFVRLTRPLAL
                     VEDTVLLAAPDDFAKSVLEQRLRPLVTEALCHELGRTVRIAVTVDATAAAGTSTPADS
                     DADGAAPVDDDPVVTAAVDDEPVEAAPAESEPTPISSIRREPGRGRRDSEPTRLNPRY
                     LFETFVIGSSNRFAHAAAVAVAEAPAKAYNPLFIYGDSGLGKTHLLHAIGHYAANLFP
                     SVRVRYVSSEEFTNDFINAIRDGRGETFRRRYREVDILLVDDIQFLENKEQTQEEFFH
                     TFNTLHNANKQIVISSDRPP"
     gene            1429..1566
                     /gene="rpmH"
                     /locus_tag="VLC50_03645"
     CDS             1429..1566
                     /gene="rpmH"
                     /locus_tag="VLC50_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013289388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HSF26592.1"
                     /translation="MSKRTFQPNNRRRAKTHGFRLRMRTRAGRAILSARRAKGRDRLS
                     A"
     gene            1587..1949
                     /gene="rnpA"
                     /locus_tag="VLC50_03650"
     CDS             1587..1949
                     /gene="rnpA"
                     /locus_tag="VLC50_03650"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010032713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HSF26593.1"
                     /translation="MLPAGHRLRRGADISTVVRRGRRATSSGARLLVVHARAADAADR
                     LPRAGFVVGRSVGGSVVRHRVIRRLRHLVRPHLATLPSGTDLVVRALPGAATASYDEL
                     DRALEGALRRATAPGRRP"
     gene            1946..2224
                     /gene="yidD"
                     /locus_tag="VLC50_03655"
     CDS             1946..2224
                     /gene="yidD"
                     /locus_tag="VLC50_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005664577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HSF26594.1"
                     /translation="MKLLLIGLIRVYQKLISPLLGPRCRFYPSCSSYAVQVLTVHGVI
                     KGSGLAAWRLLRCNPWNPGGLDPVPPRRGRADDETSAGASRPTTPQGV"
     gene            2227..3330
                     /gene="yidC"
                     /locus_tag="VLC50_03660"
     CDS             2227..3330
                     /gene="yidC"
                     /locus_tag="VLC50_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004939826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HSF26595.1"
                     /translation="MGILNPIYTAVSWVMVTWHSFWTKIGLPEDSGWAWGLSIIGLVV
                     VIRILLIPLFVKQIKAQRGLQLLQPQMKEIQKKYKNDRERQSKEMMKLYKETGTNPLS
                     SCLPLLAQSPIFFGLFHVLNGVARNPQDPPGVFTPELAESCANATIFGAPLSETFLGA
                     GTGATKAVIAVMVILMSATMFWTQKQLMSKNMPAEAMNNPFAQQQKLLLYVMPVIFLF
                     SGLGFPIGVLLYWLTSNIWTAGQQFYVIRRNPTPGSKAAIELAERRAKRGRKPAAVTS
                     GPGESAAGATAESDATVGAGQRQQPKRAPRGKRGGRPGQPSQPSQPSTAASTGQAQKS
                     DQPHQTGQNGKAKRAGKSGRPGSSGQSNRRDTA"
     gene            3448..3960
                     /locus_tag="VLC50_03665"
     CDS             3448..3960
                     /locus_tag="VLC50_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011758119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="R3H domain-containing nucleic acid-binding
                     protein"
                     /protein_id="HSF26596.1"
                     /translation="MAPESEAPESEGSERADLLARLEQEGEVAADYLEGLLDIADLDG
                     DIDMDVEGQRAAVSIVGAELTDLVGRDGQVLEALQELTRLAVAARTGERSRLMLDISG
                     HRARRREQLREQAAQAVAQVREQGSSVRMAPMTPFERKVIHDAVAEAGLRSESEGVEP
                     ERCVIVLPAS"
     gene            4125..4850
                     /gene="rsmG"
                     /locus_tag="VLC50_03670"
     CDS             4125..4850
                     /gene="rsmG"
                     /locus_tag="VLC50_03670"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018384196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HSF26597.1"
                     /translation="MSDETDRAVEVPEPPAGAARLFGDRLNLAQRYAMWLAGAGVARG
                     LIGPREVPRLWSRHLLNCAVLAELIPTGRLVADVGTGAGLPGLVLAIARDDLRVVLIE
                     PMLRRTRFLDEVVADLGLEARVTVRRARAEELRGVDADIVTSRAVAPAARLAAWSLPL
                     LRPGGLMLALKGAGAGAELAEAAPTLRRLGGEHAEIVTVGSWLEEPATVLRVVRADRD
                     GRGSGRAGQTGRAGRSGPARRSR"
     gene            5692..6696
                     /locus_tag="VLC50_03675"
     CDS             5692..6696
                     /locus_tag="VLC50_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013118915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HSF26598.1"
                     /translation="MTTRPAATNSAAPATTNQAVGRAASQVELAPVMAASDDTPIAAE
                     VADVVRRQALVGTPVPRPAHTRVLTVANQKGGVGKTTSTVNMAAALAQHGARVLVIDL
                     DPQGNASTALGIDHRGDVPSIYDVLVDGRPLAEVVAPVSAVAELYCAPATIDLAGAEI
                     ELVSLVARESRLDRALRTFLETQAASGAERFDYVLIDCPPSLGLLTVNALVAAAEVLI
                     PIQCEYYALEGLGQLLKNVELVRAHLNPNVHVSTILLTMYDARTRLAAQVAQEVRQHF
                     PELVLRTTIPRSVRISEAPSYGQTVMTYDPGSSGALSYMEAARELAERGVPTTQEERR
                     "
     gene            6693..7796
                     /locus_tag="VLC50_03680"
     CDS             6693..7796
                     /locus_tag="VLC50_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019135611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HSF26599.1"
                     /translation="MSDRRRGLGRGLGALIPTGPARPEAGAARLGDSGPSGVDVLIPT
                     RPGVAEAGTADAASATGAASTAVTDPAGVPADGAADQGAADQSAAGRPEVAPVPGAYF
                     AELPVEQIVPNARQPRQVFDEEAMAELVHSLREIGLLQPIVVREVGPQHYELIMGERR
                     WRAAQLAGFTRIPAIVRSTDDDAMLRDALLENLHRSQLNPLEEAAAYQQLLDDFGCTH
                     EELAGRIGRSRPQISNTLRLLKLPPTVQRRVAAAVLSAGHARALLGLAEADAQERLAA
                     RIVAEGLSVRAVEEIVAVGDDAPATRSRSRANRPVAPGLVELAERLSDRFETRVSVNL
                     GRRRGKIVVEFASIADLERIVGLLDASGPAAAD"
     gene            complement(7822..8781)
                     /locus_tag="VLC50_03685"
     CDS             complement(7822..8781)
                     /locus_tag="VLC50_03685"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012855272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HSF26600.1"
                     /translation="MSDLGRVVVLAGGLSHERDVSLRSGRRVSEALRAVGIDVDERDV
                     GPELVSTLVADPPAAVFPVLHGAAGEDGSVREVLELLHTPYVGSGPAAARVAWDKPTA
                     KTVVAGAGIATPASVTLPEATFRDLGAGAVLDAVLERLGLPLVVKPARGGSALGCTVV
                     HRAEDLPAAMVTCFAYGDTALLERFVTGTEVAVSVVDTGDGPRALPAVEIVPDGGVYD
                     YAARYTAGTTEFFVPARLDPTLADAVEQVAVRAHRALGLRDLSRSDLIVDATGQVQFL
                     EVNVAPGLTETSLLPQAADAVGLELGTLYRDLAALAVSRLAGS"
     gene            complement(8802..10115)
                     /locus_tag="VLC50_03690"
     CDS             complement(8802..10115)
                     /locus_tag="VLC50_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010234548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HSF26601.1"
                     /translation="MSGSPAGSRLDLYVDRYAARTHGMTASEIRALFAVASRPEVVSL
                     AGGMPNISGLPLDVVSSMISELVVEHGAVALQYATAQGDPHLREQICEVMRLEGISAH
                     PDDVVVTVGSQQALDLVTRIFVNPGDVVFAEAPSYVGALGTFRAYQAEVVHVAMDDDG
                     LVPEHLRQAIASVKAAGKRAKFLYTIPNHHNPAGISLTASRRAEILEIAREADLLVLE
                     DNPYGLLGFDADPIPAMRAQEADRVIYLGSFSKTFAPGLRVGWAVAPHAVREKLVLAQ
                     ESATLCPPTFSQLAISAYLDGHDWRGQIKGFREMYRERRDAMLAALDDLMPAGCTWTK
                     PAGGFFVWLTLPEGLDAKAMLPRAVTNRVAYVPGTGFYSDGFGSRCMRLSYCYPTPER
                     IWEGVRRLAAVLRQEIALRETFGPTAISAPSDESPAVQAPGPETT"
     gene            10282..10407
                     /locus_tag="VLC50_03695"
     CDS             10282..10407
                     /locus_tag="VLC50_03695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF26602.1"
                     /translation="MRLLRRVLTGLVIGAGVGYVVELLRPRRHRVAGDGGGPRGF"
     gene            10409..11035
                     /locus_tag="VLC50_03700"
     CDS             10409..11035
                     /locus_tag="VLC50_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015626265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HSF26603.1"
                     /translation="MRQLANLTLDNLNDLTPRCRACVFWELDPVAGERASASEQAALE
                     KESWVSTTLLEWGSCGKIVYVDSRPAGYVLYAPPSYVPRSRAFPTSPVSADAVLLMVA
                     DVRPEYAGGGLGRVLVQTVAKDLTLRGVKAMEAFGHTHDRADPVHPSCLVPADYLLAV
                     GFKTVRPHHRVPRLRLDLRTALRWREDVEVAIERLLGSMAPAPATLRG"
     gene            complement(11040..11366)
                     /gene="trxA"
                     /locus_tag="VLC50_03705"
     CDS             complement(11040..11366)
                     /gene="trxA"
                     /locus_tag="VLC50_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013875632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HSF26604.1"
                     /translation="MGAHTPPVTDATFTDEVLAAEGTVIVDFWAEWCGPCRQVGPILD
                     EIAAEHADKIRILKLNVDENPQVAAKYGITSIPTMNVYSGGEVVKSIVGAKPKAMLLR
                     DLAAFI"
     gene            complement(11416..12396)
                     /gene="trxB"
                     /locus_tag="VLC50_03710"
     CDS             complement(11416..12396)
                     /gene="trxB"
                     /locus_tag="VLC50_03710"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012924603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HSF26605.1"
                     /translation="MSDVRDVIIIGSGPAGYTAAIYAARAQLAPLVFESSVAAGGALM
                     NTTEVENFPGFPDGIIGPELMDGLRKQAERFGAELVADDIFEVDLTGPIKRVTDGSGT
                     THEARAVILAMGSAYRKLGLPNEDRLSGHGVSWCATCDGFFFREQDIAVIGGGDSAVE
                     EATFLTRFARKVYLVHRRDELRASKIMAERALTNPKIEMVWNSEVVEILGDAKLSGLR
                     LRDTVTGEERSLDVTGLFIAIGHDPRSALVRGQVDLDANGYVVVEGRTQRTNLAGVFA
                     AGDVVDHTYRQAITAAGHGCAAALDAEHYLAALDDAGDQSTAAVTETATV"
     gene            complement(12521..13432)
                     /locus_tag="VLC50_03715"
     CDS             complement(12521..13432)
                     /locus_tag="VLC50_03715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF26606.1"
                     /translation="MTTPSGPDESTHLSADVLADLDEGLLATTEADRARTHLLMCPAC
                     AGEHAALAELRQALAADDPGPMPADVVARLESALRAAEHPVDHPADRPAAAVTTLATR
                     RAGRGPGRPPRWLMAAAAVTALVVAGGAVVAALQAGRDSGAVTASESAAQDSAAGAAG
                     APGAAGGPLVTRSGTDYDAASVETATRQLTSGGYAALGGTRDQAPASADPQAPTLAGG
                     PAEQSAAAALDRLAAGPGLTACLAQLTDDGEAPIAVDLARFDGQPAALVVLPTPGETT
                     TVDAWVVGPGCGEGGDAQVLYFRRVPR"
     gene            complement(13429..14097)
                     /gene="sigM"
                     /locus_tag="VLC50_03720"
     CDS             complement(13429..14097)
                     /gene="sigM"
                     /locus_tag="VLC50_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018654228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor SigM"
                     /protein_id="HSF26607.1"
                     /translation="MPDGDPDRLADLDDQALMARHADGDDAAFAEVVRRHRDRLWAVA
                     LRTVGDREEAADAVQDALLSALRAGRSGAFRGDAAVTTWLHRIVVNACLDRVRRRSVR
                     PADPLGEDAARLPDPVDPVGRQETAIVVAAALATLPVEQRAALVLVDMHGYSVEEAAR
                     LLGCAPGTIKSRCARGRAKLLPVVSDLRAARNHGPGGDVPADERTSPAGRTPRHDEEA
                     GERR"
     gene            complement(14201..15709)
                     /locus_tag="VLC50_03725"
     CDS             complement(14201..15709)
                     /locus_tag="VLC50_03725"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase family protein"
                     /protein_id="HSF26608.1"
                     /translation="MPTSPPSSSLGPGTRLAGRYRIEDHVIDLATTSYWRGVDETLGR
                     DVGVRTVAAADPLAATLTAAARAAAASHDPRFLRVLDVATDQGQVYVVSEWASGPSLE
                     ELLNTGPLPDKDAARVAREVAEALATAHHQQLWHRCLRPASVVVTDAGQVRLVGLCVD
                     AAVAGLPEITETEAAREDAAGCGRLLFAALTGRWPGERQTPHGSTLPPPPVADGRAVS
                     PRQVRAGVPAGLDAVTMRALGQPAHRESPLSSPAAVAAALTHVQPTEPDHRDNPTAYD
                     GQVAASVVPPNVPPELTPRPAPTTGTSWAGWAVAAVLVVAVGLLGWAIVATVSGSLSQ
                     TDRGGGTPTTATTEASGPPLGALIPVSALTDFDPQGNGEENRDKLDLAIDGDASTAWT
                     TMTYYNRANLGGLKDGVGVLVDLGSPQSVGSVSLELVGRGTSVALHAGDQRPRSVDEL
                     TRVAERDDAGTNVTLRPNKPVTARYLLVWLTKLPSIGGTDYRGGIAELSVHH"
     gene            complement(15780..16871)
                     /locus_tag="VLC50_03730"
     CDS             complement(15780..16871)
                     /locus_tag="VLC50_03730"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HSF26609.1"
                     /translation="MIRPGAHGRTVLTALAALATAASLLVVPTGPAVADPDLAAARQQ
                     ASALRTQVEALTVAAELATEHYDGITDQLATLATREGLARRQLDAARVAHDGSLREVE
                     ARARAIYMAGGTAGLYATLLDGQDIGDLLSRIAAVHAVMDASSIDVADRAAVTDQAAD
                     IALELGQLADQQSALEAQAEDAAAEVTRLLDQSQALLEQADARVRTLVADEAARAHAL
                     AWASSQTRLAELGLLQADAPPGTGWAPQAIAAARAMIGRPYQWGAVGPETFDCSGLTG
                     WAYRQAGLSLPRTSRQQWFAGRAVPLNELAPGDLLFWATDVTNPSTIHHVGIYVGGGR
                     MIDAPRTGMPVRETGVLLDGLIGAIRPGV"
     gene            complement(16868..17260)
                     /locus_tag="VLC50_03735"
     CDS             complement(16868..17260)
                     /locus_tag="VLC50_03735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF26610.1"
                     /translation="MNDDRGDIILGWLAKVVVTLALVGLVIFDLISIGWTKVSLTDQA
                     KAAAISASTAWAHGHNVQKAYDAALAQATEDNAANTVATEGFTVDPDGTVHLRLDREA
                     STIVVSRIGPIRDWGQLTATASGRDVTG"
     gene            17409..>17668
                     /locus_tag="VLC50_03740"
     CDS             17409..>17668
                     /locus_tag="VLC50_03740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF26611.1"
                     /translation="MFDNTGDGPGADAIGSKPTDTSHTDTSDTEPLAPADPLAPADPL
                     LPVDPPQPAVSTPEPPALPGLAGRLLAGLDPASAAAVRAAVS"
BASE COUNT         2327 a         6251 c         6624 g         2466 t
ORIGIN      
        1 cggcggccgg tcgctggaga tgacgatctg cttgttggcg ttgtgcagcg tgttgaaggt
       61 gtggaagaac tcctcctgcg tctgctcctt gttctccaag aactggatgt cgtcgacgag
      121 caggatgtcg acctcgcggt agcggcggcg gaacgtctcg ccccggccgt cgcggatcgc
      181 gttgatgaag tcgttggtga actcttccga gctcacgtag cgcacccgca cgctcgggaa
      241 caggttggcc gcgtagtggc cgatcgcgtg gagcaggtgg gtcttgccca gcccggagtc
      301 gccatagatg aacagcgggt tgtaggcctt ggccggcgcc tcggccaccg cgaccgcggc
      361 cgcgtgggcg aaccggttgc tcgaaccgat gacgaacgtc tcgaacaggt agcgcgggtt
      421 gagccgggtc ggctcgctgt cacggcgccc gcggccgggc tcgcgccgga tcgaggagat
      481 cggcgtcggc tcgctctcgg ccggtgccgc ctcgaccggc tcgtcgtcga ccgccgctgt
      541 gacgaccggg tcgtcgtcga ccggggccgc gccgtcggcg tcggagtcgg ccggtgtgct
      601 cgtgccggcg gccgcggtgg cgtcgaccgt caccgcgatg cgcacggtgc ggcccagctc
      661 gtggcagagc gcctcggtga ccagcggacg cagccgttgc tcgaggacgc tcttggcgaa
      721 gtcgtcgggg gccgcgagca gcacggtgtc ctcgacgagg gccagcgggc gggtcagccg
      781 gacgaaggcc cggtggttgg gggcgacgtc gtcaccggtg agcgtggcca gggagcgtgt
      841 ccagacctcg gctaggttga tctctcctgc cggcgcagac tcgctcacct ggttgttctc
      901 cctcctgtga cgccgccgca cgaaccatga agtcgggcac gggccgggag agtcgctcgg
      961 tccacaggcg cgccgtccac acacctttcc acagcctgtg gagggcgagg ggtatggggg
     1021 caggggagcc tatgccgtga ccgttgcgat gccccctggt gcgctcgatc tggcggtgct
     1081 ccacgacgcg gcccgcgacg ccgacgtacg gcgtagccgg gctgaaccga atcgtgacgc
     1141 tagtagacgt ctgccccggc ggcaaccggt tcgagcaaac atgcgcctga acggtgcagt
     1201 cgtggtttga ccgtcggcgg ggcggcgccg taccgttgtc cggtcgctcg agtcgacgcc
     1261 tgtgcatgca tgccctggga tctgttctgt gtggccccaa ggcggcgagg cggttgcgag
     1321 cgcacctgtc tcatcgtggc cagcaggcca cccggagcac ccgtcgggcg cgtcacgcgt
     1381 ggccacggcg atgccctggg ccgagtgacc cgtgcagggg agtatgtcgt gagcaagcgg
     1441 acgttccagc cgaacaaccg ccgccgggcc aagacccatg gttttcgcct gcgcatgcgg
     1501 accagggccg ggcgcgcgat cctctcggcc cgtcgggcca agggtcgcga ccggttgtcg
     1561 gcctgagtca ccgcggagcc aggctcgtgc tgcctgccgg ccaccggctg cggcgcgggg
     1621 ccgacatctc caccgtcgtc cgtcgcggac gccgggcgac cagttccggg gcgaggctgc
     1681 tcgtcgtgca cgctcgggcg gccgacgccg ctgatcgcct cccgcgggcc ggtttcgtcg
     1741 tgggccgctc ggtcggcggc tctgtcgtgc gccaccgggt gatccgccgc ctgcgccacc
     1801 tcgtgcgccc ccacctggcc accttgccgt ccggcaccga cctggtcgtc cgggccctgc
     1861 cgggagcggc caccgcgtcg tacgacgagc ttgaccgcgc gctcgagggg gcgctgcgcc
     1921 gcgccaccgc accggggcgg cgcccatgaa gctcctgctg atcggcctga tccgggtcta
     1981 ccagaagctg atctctccgc tgctggggcc gcgctgccgc ttctacccct cctgctcctc
     2041 ctacgccgtg caggtcctga cggtccacgg tgtgatcaag ggttccgggc tggccgcttg
     2101 gcggctgctg cgctgcaacc cgtggaaccc cggcgggctg gaccccgttc cccctaggcg
     2161 tggccgcgcc gatgacgaga cctccgccgg cgcgagccgg cccacgaccc cgcaaggagt
     2221 ctgaccttgg gcatcctcaa cccgatctac acggctgtct cgtgggtcat ggtgacctgg
     2281 cacagcttct ggacgaagat cggcctgccc gaagactccg ggtgggcgtg gggcctgtcg
     2341 atcatcggcc tggtcgtggt catccgcatc ctgctgatcc cgctgttcgt caagcagatc
     2401 aaggcgcagc gcggcctgca gctgctgcag ccgcagatga aggagatcca gaagaagtac
     2461 aagaacgacc gggaacgcca gtcgaaggag atgatgaagc tctacaagga gaccggcacc
     2521 aaccccttgt cgagctgcct ccccctgctg gcgcagtccc cgatcttctt cggcctcttc
     2581 cacgtcctca acggcgtcgc ccgaaacccg caggatccgc ccggcgtgtt cacgccggag
     2641 ctggccgagt cgtgcgccaa cgccaccatc tttggcgcgc cactgtcgga gacgttcctc
     2701 ggcgccggca ccggggcgac caaggcggtc atcgccgtca tggtcatcct catgtcggcg
     2761 accatgttct ggacgcagaa gcagctcatg tccaagaaca tgcctgccga ggcgatgaac
     2821 aacccgttcg cccagcagca gaagctgctc ctctacgtca tgccggtgat cttcctgttc
     2881 tccggcctgg gcttcccgat cggcgtcctg ctctactggc tgacctccaa catctggacc
     2941 gccggtcagc agttctacgt gatccgacgc aacccgaccc cggggtcgaa ggcggcgatc
     3001 gagctggccg agcggcgggc caagcgtggg cgcaagccgg ccgccgtgac gagcgggccg
     3061 ggcgagtcgg ccgccggcgc caccgcggaa tcggacgcaa cggtgggagc gggccagcgc
     3121 caacagccca agcgggcccc gcgcggcaag cgcggcggcc gccccggcca gccctcgcag
     3181 ccgtcgcagc cgtcgaccgc tgcctcgacg gggcaggcgc aaaagtcgga ccaaccccat
     3241 caaactggtc aaaacggaaa ggcgaagcgg gccggcaagt ccggtcgacc cgggtcgtcc
     3301 ggccagtcga accggcgtga caccgcctga gaccgctgac ttcacgactg ctgacacgaa
     3361 agattggtgc aatccgtgag cgagacgacg acccctgagc ccgccagctc ggattcggtg
     3421 ctatcggagt cggtggcgcc cgagtcgatg gcgcccgagt cggaggcgcc cgagtcggag
     3481 ggcagcgagc gcgccgacct gctcgctcgg ctcgagcagg agggcgaggt ggccgccgac
     3541 tacctcgagg gcttgctgga catcgccgac ctcgacggtg acatcgacat ggacgtcgag
     3601 ggccagcgcg cggccgtctc catcgtcggc gccgagctga ccgacctggt gggtcgcgac
     3661 gggcaggtgc tcgaggcgct gcaggagctc acccggctcg cggtggccgc ccgtaccggt
     3721 gagcggagcc gactgatgct cgacatctca gggcaccggg cgcgtcgacg tgagcagctg
     3781 cgcgagcagg ccgcccaggc cgtcgcacag gtgcgcgagc agggctcgtc ggtgcggatg
     3841 gctccgatga ccccgtttga gcgcaaggtc atccacgacg cggtggccga ggcgggactg
     3901 cgcagcgagt ccgagggcgt cgagcccgag cgctgcgtga tcgtgctgcc ggcctcctga
     3961 cgcgcaccag ctgactgctg tgtacggcgc ctcgtgagcg caccgcgcga tcgggtgctc
     4021 tcgtttcacg tgaaaccgca tgacggcgcc gccgcgcccg atcatcaatg tgcccccgcc
     4081 cggacaaggc acagcggccg tgcccgcgca aggtggtcgg tccgatgagc gacgagacag
     4141 atcgcgccgt ggaggtgccg gagccaccag cgggtgccgc gaggctgttc ggtgaccggc
     4201 taaacctggc ccagcgctat gccatgtggt tggccggcgc cggggtggcg cgcgggctga
     4261 tcggaccgcg ggaggtgccg cggctgtggt cacgccacct gctcaactgt gcggtactcg
     4321 ccgagctgat ccccacgggt cgactcgtcg ccgacgtggg gaccggcgcc ggacttcccg
     4381 gcctcgtgct ggcgatcgcg cgcgacgacc tgcgcgtcgt gctcatcgag ccgatgctgc
     4441 ggcgcacccg attcctcgac gaggtggtcg ccgacctggg gctcgaggct cgggtcacgg
     4501 tccgacgggc gcgggccgaa gagctgcgcg gggtcgacgc cgacatcgtc acgtcgcggg
     4561 cggtggcccc cgcggcgcgg ctggccgcct ggtcgctgcc gctgttgcgt cccggtgggc
     4621 tgatgttggc cctcaaggga gcaggggcgg gggccgagct cgcggaggcg gcgccgacgc
     4681 tgcgtcggct gggcggcgag catgccgaga tcgtcaccgt cggttcctgg ctcgaggagc
     4741 cggcgacggt gctgcgggtc gtgcgcgcgg atcgcgacgg ccggggttcc ggtcgggccg
     4801 gccagaccgg tcgggccggc cgatccgggc ccgcccgacg ctcgcggtga ggcggcccac
     4861 gggccgctct tcagggcaga ggcctccctt gtggatgaag gtgtgacaag aggggtcaag
     4921 cgcgggcagg atggggcggc gacgtagagg agtggtgcgg tgactggcgg gctcggatgg
     4981 ccatggtcga cgacgcatgc cgatgacgac tcatcgccgg gtctcggctg gccgacgggt
     5041 accgacgagc cgagcggatc ggcgaccgtt tcacgtgaaa cgggcggggg agagcgaccg
     5101 ggcgcagcca gtgcagccgc cgcccagccg gcagacgaga cccggccggc tgacgaggcg
     5161 cgaccgccgc agccggccag accgtccgcg ccgctgaacg gtcaccacgc cggtcctggt
     5221 ccggcgccca agccggatct cgacgggccc tccgcttcgg ggcccgccgg ctcggtggct
     5281 attggctcgg tggctattgg ctcagcggcc gtcgactcag caactgccgg ctcccccggc
     5341 gatgttgccg ccacacgtcc ttggggtcgc aaggcgttcg gcccggcgtt ccgggccacc
     5401 ccacgggagt cgacgacccc acacgagtcg acgaatgccg gcgatcccgt cgtcgtcgac
     5461 ggacacccaa agagcggatt cgcggacacg tccgagggca ggacatcgag ctcggccccg
     5521 gctcaaccca ctcagtccac tccgtccacc gagccggttt cacgtgaaac gtcgtcgacg
     5581 cctccggcgc cgccgacgac agacgcgtca gtcgctgagg ctcccgcggt ttcacgtgaa
     5641 acggacgcgt cgccgaccgc cgacccggca ggcgccgggc acgtcttggt catgaccacc
     5701 cgaccggccg cgacgaattc ggcagcaccg gcgacgacca atcaggccgt cggccgcgcc
     5761 gccagccagg tcgagctggc cccggtgatg gcggcttcgg atgacacccc gatcgccgcc
     5821 gaggtcgccg acgtcgtacg ccgtcaggcc ctggtcggca cgcccgtgcc gcggccggcg
     5881 cacacccggg tgctcacggt ggccaaccag aagggtgggg tcggcaagac cacgtccacg
     5941 gtcaacatgg cggccgcgct ggcccagcac ggggcgcggg tgctggtgat cgacctcgac
     6001 ccgcagggga acgcgtcgac ggcgctgggc atcgaccacc gcggagacgt gccctcgatc
     6061 tacgacgtgc tggtcgacgg gcgaccgctc gccgaggtgg tcgcgccggt ctccgccgtg
     6121 gccgagctct actgcgcacc ggcgaccatc gacctggccg gcgcggagat cgagctcgtg
     6181 tcgttggtcg ccagagagtc ccggctggac cgggcgttgc gcacgttcct ggagacccag
     6241 gcggcgtcgg gggccgagcg cttcgactac gtgctgatcg actgcccgcc ctcgctcggg
     6301 ttgctcaccg tcaacgccct ggtcgccgct gccgaggtgc tgatcccgat ccagtgcgag
     6361 tactacgcgc tggaggggct cggccagctg ctcaagaacg tcgagctggt gcgtgcccac
     6421 ctcaacccca acgtgcacgt ctcgacgatc ctgctcacga tgtacgacgc ccggacccgg
     6481 ctggccgctc aggtcgccca ggaggtgcgc cagcacttcc cggagctcgt gctgcgcacc
     6541 acgatcccgc ggtcggtgcg catctccgag gcgcccagct atgggcagac cgtgatgacc
     6601 tacgaccccg gctcgagcgg ggccttgtcc tacatggaag cggcccgcga gcttgctgag
     6661 cgcggggtcc ccacaacgca ggaggagcga cggtgagtga tcgacggcgt gggctgggtc
     6721 gtggactcgg cgcgctcatc cccaccgggc cggcccggcc ggaggcaggt gcggcccggc
     6781 tgggggactc cgggccctcc ggtgtcgacg tgctgatccc gacccgacca ggggtggccg
     6841 aggcagggac ggcggatgcg gcgagcgcga cgggtgccgc gtcgacggcg gtcaccgacc
     6901 cggccggcgt gccggcggac ggcgcggccg accagggtgc ggcagaccag agtgccgccg
     6961 gccggcccga ggtggccccg gtgcccggcg cctacttcgc cgagctgccg gtggagcaga
     7021 tcgtccccaa cgcccgccag ccgcggcagg tcttcgacga ggaggcgatg gccgagctcg
     7081 tccactcgct gcgtgagatc gggctgctgc agccgatcgt ggtccgcgag gtgggcccgc
     7141 agcactacga gctgatcatg ggtgagcggc gctggcgggc ggcccagctc gccgggttca
     7201 cccggatccc cgcgatcgtg cgctccaccg acgacgacgc gatgctgcgc gacgcactgc
     7261 tggagaacct gcaccgctcg cagctcaacc cgctggagga ggcggcggcc taccagcagc
     7321 tgctcgacga cttcggctgc acgcacgagg agctggcggg ccggatcggg cgcagccggc
     7381 cccagatctc caacacgctc cggttgctca agctgccgcc gacggtccag cggcgggtcg
     7441 ccgccgccgt gctctcggcc gggcacgccc gggccctgct cgggctggcc gaggccgacg
     7501 cgcaggagcg gctggccgca cggatcgtcg ccgaggggct gtcggtgcgc gccgtcgagg
     7561 agattgtcgc ggtgggggat gacgcgccgg ccacccggtc acgcagccgc gccaaccgtc
     7621 cggtcgctcc ggggctggtc gagctggccg agcggctgtc cgaccggttc gagacccggg
     7681 tgtcggtcaa tctgggccgg cgccgcggca agatcgtcgt ggagttcgcc tcgatcgccg
     7741 acctcgagcg gatcgtcggg ctgctcgacg ccagcggacc ggccgccgcc gactaggggc
     7801 gggccggccg gggcagcagc ctcagctgcc ggcgaggcgg gagaccgcca gggcggccag
     7861 gtcgcgatag agcgtgccca gctcgagccc gacggcgtcg gcggcctgcg gcagcagcga
     7921 ggtctcggtc aggccgggcg cgacgttgac ctcaaggaac tgcacctggc cggtggcgtc
     7981 gacgatgagg tcggagcgcg agaggtcgcg caggcccagc gcccggtggg cgcgcaccgc
     8041 gacctgctcg accgcatcgg ccagcgtggg gtcgagccgg gcgggcacga agaactcggt
     8101 cgtgccggcg gtgtagcgcg cggcgtagtc gtagaccccg ccgtcgggca cgatctcgac
     8161 cgccggcagc gcgcgcggcc cgtcaccggt gtccacgacg ctcaccgcga cctcggtgcc
     8221 ggtgacgaat cgctcgagca gcgcggtgtc gccgtaggcg aagcaggtga ccatcgcggc
     8281 cggcaggtcc tcggcccggt gcacgacggt gcagcccagc gccgagccgc cgcgggccgg
     8341 cttgaccacg agcgggaggc cgagacgctc cagcaccgcg tcgagcaccg cgccggcgcc
     8401 caggtcgcgg aacgtcgcct cgggcagggt caccgacgcg ggggtggcga tgccggcccc
     8461 ggcgaccacc gtcttggcgg tcggcttgtc ccaggccacc cgggccgccg ccgggccgga
     8521 gccgacgtac ggcgtgtgca gcagctcgag cacctcgcgc accgagccgt cctcgccggc
     8581 cgcgccgtgc agcaccggga acaccgcggc gggcgggtcg gcgaccagcg tcgagaccag
     8641 ctcgggcccg acgtcgcgct cgtcgacgtc gatgcctacc gcgcgcagcg cctcgctgac
     8701 ccgccgcccg gagcgcaacg acacgtcgcg ctcgtgggag agcccgccgg ccagcactac
     8761 gacacgtccc aggtcgctca tggatctgct cgccttctcg ttcacgtggt ctcggggccc
     8821 ggtgcctgca ccgcggggct ctcgtcgctg ggcgcgctga tcgcggtcgg cccgaacgtc
     8881 tcgcgcagcg cgatctcctg gcgcagcacc gccgccagcc gccgtacgcc ctcccagatc
     8941 cgctccgggg tggggtagca gtaggacagc cgcatgcacc ggctgccgaa cccgtcggag
     9001 tagaagccgg tgccgggcac gtaggccact cggttggtca ccgcgcgggg gagcatcgcc
     9061 ttggcgtcca ggccctcggg cagggtcagc cagacgaaga acccccccgc cggcttggtc
     9121 caggtgcagc cggccggcat caggtcgtcg agcgcggcga gcatcgcgtc gcggcgctcg
     9181 cggtacatct cgcggaagcc cttgatctgc ccgcgccagt cgtgtccgtc gaggtaggcc
     9241 gagatcgcca gctggctgaa cgtgggcggg cacagcgtcg ccgactcctg ggccagcacg
     9301 agcttttcgc gtacggcgtg cggggcgacc gcccagccga cccgcagccc gggggcgaag
     9361 gtcttggaga acgagcccag gtagatcacc cggtcggcct cctgggcgcg catcgccggg
     9421 atcgggtcgg cgtcgaagcc gagcaggccg taggggttgt cctcgagcac cagcaggtcg
     9481 gcctcgcggg cgatctcgag gatctcggcc cggcggctgg cggtcagcga gatgcctgcc
     9541 ggattgtggt gatttgggat ggtatagagg aacttggcgc gcttgccggc cgccttcacc
     9601 gaggcgatcg cctggcgtag gtgctcgggg accagcccgt cgtcgtccat cgccacgtgc
     9661 acgacctcgg cctggtaggc ccggaacgtg cccagcgcgc cgacgtagga cggcgcctcg
     9721 gcgaacacca cgtcgccggg gttgacgaag atccgggtga ccaggtcgag ggcctgctgc
     9781 gagccgaccg tgaccaccac gtcgtcgggg tgcgcgctga tgccctccag gcgcatcacc
     9841 tcgcagatct gctcgcgcag gtgcgggtca ccctgggcgg tggcgtactg cagggcgacc
     9901 gcgccgtgct cgaccaccag ctcagagatc atcgacgaga cgacgtcgag cggcagcccg
     9961 gagatgttgg gcatcccgcc ggccagcgag acgacctcgg ggcgcgaggc gacggcgaag
    10021 agagctcgga tctcggaggc ggtcatccca tgggtccggg ccgcatagcg gtcgacgtac
    10081 aggtcaagcc gggacccggc gggtgaaccg ctcacgggaa cctcctcgcg tgccgatgct
    10141 ggtgccgcgt acgccggccg gctcgcgcgc gccgggtcca cgcggccctg tcagcgggcc
    10201 gagggcccga ctacgatcgc gaggggtcga gtcgaggccg gacgggggcg gacaacgccg
    10261 gccgccgaag ggaagtgtcc catgcgtctg ctccgtcgtg tcctgaccgg gctggtgatc
    10321 ggcgccggtg tcggctacgt cgtcgagctg ctgcggcccc gccgtcatcg ggtcgccggc
    10381 gatggtggtg gcccgcgagg gttctgagat gcgccagctg gccaacctga ccctcgacaa
    10441 cctcaacgac ctgaccccac gctgtcgcgc gtgcgtgttc tgggaactcg acccggtggc
    10501 cggcgagcgg gcgagcgcga gcgagcaggc ggcgctggag aaggagtcct gggtctccac
    10561 cacgctgctc gagtggggct cctgcggcaa gatcgtctac gtcgactcgc ggccggccgg
    10621 ctacgtcctc tacgccccgc cgtcctacgt gccgcgctcc cgggcgttcc cgaccagccc
    10681 ggtcagtgcc gacgcggtgc tgctcatggt cgccgacgtg cgccccgagt acgccggcgg
    10741 cgggctgggc cgggtgctcg tgcagacagt ggccaaggac ctcacgctgc gcggggtcaa
    10801 ggcgatggag gcgttcgggc acacccacga ccgggccgac ccggtgcacc cgtcgtgcct
    10861 ggtgccggcc gactacctgc tcgcggtcgg cttcaagacg gtccgcccgc accaccgggt
    10921 gccccggctg cggctcgacc tgcgcactgc gctccgctgg cgggaggacg tcgaggtggc
    10981 catcgagcgc ctgctcggct cgatggcccc ggctcctgcc accctgcgcg ggtgatcgtt
    11041 cagatgaagg cggccaggtc gcgcagcagc atcgccttcg gcttggcgcc gacgatcgac
    11101 ttgaccacct cgccgcccga gtagacgttc atcgtcggga tcgaggtgat gccgtacttc
    11161 gcggccacct gcgggttctc gtcgacgttg agcttgagga tgcggatctt gtcggcgtgc
    11221 tcggcggcga tctcgtcaag gatcgggccg acctgccggc acgggccgca ccactcggcc
    11281 cagaagtcga cgatgacagt gccctcggcc gcgaggacct cgtcggtgaa cgtggcgtcg
    11341 gtgacgggtg gggtgtgggc gcccacggag tgctcctctg gtcgggttcg ggtcgtgcgg
    11401 tggtctttgt cgggcttaga ccgtcgcggt ctcggtgacc gcagcggtgg actggtcgcc
    11461 ggcgtcgtcg agggcggcca ggtagtgctc ggcgtcgagc gcggccgcgc agccgtgtcc
    11521 ggccgcggtg atggcctgcc ggtaggtgtg gtcgacgacg tcgccggcgg cgaagacccc
    11581 ggccaggttg gtgcgctggg tgcgcccctc gacgacgacg tagccgttgg cgtcgagatc
    11641 gacctgccca cgcaccagcg cggaccgcgg gtcgtgaccg atcgcgatga acagcccggt
    11701 cacgtcgagc gagcgctcct caccggtcac cgtgtcgcgc agccgcaggc cggacagctt
    11761 ggcgtcaccg aggatctcga ccacctcgga gttccacacc atctcgatct tggggttggt
    11821 cagcgcccgc tcagccatga tcttgctggc ccgcagctcg tcacggcggt ggaccaggta
    11881 gaccttgcgc gcgaaccggg tgaggaaggt ggcctcctcg accgcggagt cgccaccgcc
    11941 gatcaccgcg atgtcctgtt cgcggaagaa gaagccgtcg caggtggcgc accaggagac
    12001 cccgtgcccc gacagccggt cttcattggg caggccgagc ttgcggtagg ccgagcccat
    12061 cgccaggatc accgcgcgcg cctcgtgggt ggtgccggat ccgtcggtga cccgcttgat
    12121 cggcccggtc aggtcgacct cgaagatgtc gtcggcgacc agctcggcgc cgaaacgctc
    12181 ggcctgcttg cgcagcccgt ccatgagctc ggggccgatg atgccgtcgg ggaagcccgg
    12241 gaagttctcg acctcggtcg tgttcatgag cgccccgccg gcggccaccg agctctcgaa
    12301 gaccagcggc gcgagctggg cccgtgcggc atagatcgcg gcggtgtagc cggccggccc
    12361 cgagccgatg atgatcacgt cacggacgtc gctcacagct gcctctcgtc gtacgtctgg
    12421 gagaacctgc gctcctgcgg gcagaacgtg agtctagggg cgccacattc ccatggacgg
    12481 cggcgagcca ccgcacaccg gtcagaaccg gacaccggcg tcagcggggg acgcggcgga
    12541 agtagagcac ctgggcgtcg ccgccctcgc cgcagcccgg accgaccacc caggcgtcga
    12601 cggtggtcgt ctcgccgggg gtgggcagta cgacgagcgc ggccggctgg ccgtcgaagc
    12661 gggccaggtc gacggcgatc ggagcctcgc cgtcgtcggt gagctgggcc aggcaggcgg
    12721 tcagcccggg gccggccgcg agccggtcga gcgcggccgc ggccgactgc tcggcagggc
    12781 cgccggccag ggtgggcgcc tgcggatcgg ccgacgccgg cgcttggtcg cgggtgccgc
    12841 cgagcgcggc gtagccgccc gaggtgagct ggcgggtggc cgtctccacg ctggccgcgt
    12901 cgtagtcggt gccggagcgg gtcacgagcg ggccaccggc cgcaccgggc gcaccggccg
    12961 caccagccgc cgagtcctgc gccgccgact cgctggcggt cactgctccg gagtcgcggc
    13021 cggcctgcag cgcggccacg accgcgccac cggccacgac cagggcggtc accgcggcgg
    13081 ccgccatcag ccagcgcggc ggtcggcccg ggccccgccc cgcccgccgg gtcgccaacg
    13141 tggtcacggc cgcggccggt cggtcggcgg ggtggtcgac cgggtgctcg gcggcgcgca
    13201 gagccgactc cagccgggcg actacgtcgg ccggcatcgg cccggggtcg tcggcggcca
    13261 gcgcctggcg cagctcggcc agcgcggcgt gctcacccgc gcaggccggg cacatcagca
    13321 ggtgggtgcg agcccggtcg gcctcggtgg tcgcgagcag gccctcgtcg aggtcggcga
    13381 gcacgtcggc ggacaggtgg gtcgactcat cgggaccaga cggcgtggtc accggcgctc
    13441 acctgcctcc tcgtcgtgcc gcggggtgcg ccctgcgggg ctcgtgcgtt cgtctgctgg
    13501 gacgtcgccg ccgggtccgt ggttccgcgc cgcgcgcagg tcgctgacca ccggcagcag
    13561 cttggcccgc ccacgcgcgc agcggctctt gatcgtgccc ggcgcgcacc cgagcagccg
    13621 ggccgcctcc tcgaccgagt agccgtgcat gtcgacgagc acgagcgcgg cccgttgctc
    13681 gaccggcagg gtggccagcg ccgccgccac cacgatcgcg gtctcctggc gcccgaccgg
    13741 gtcgacgggg tcgggcagcc gcgcggcgtc ctcgccgagc gggtcggccg ggcgcaccga
    13801 ccggcgtcgt acccggtcga ggcaggcatt gaccacgatc cggtgcagcc aggtggtgac
    13861 cgccgcgtca ccgcggaacg ccccggagcg cccggcccgc agcgcggaga gcagggcgtc
    13921 ctgcacggcg tcggcggcct cctcccggtc gccgacggtg cgcagcgcga ccgcccacaa
    13981 ccggtcgcgg tggcgacgga ccacctcggc gaacgccgcg tcgtcgccgt cggcgtgccg
    14041 ggccatcagg gcctggtcgt cgaggtcggc gagacggtcg gggtcgccgt cgggcatgcg
    14101 tcgtcccttt cgcgtgcgat cgggtgggtg tccaccaggg tcgcatgcca ggggtacgac
    14161 gacccggcag gcgcaccgga ggcggtcggg cgcgggccgg tcagtggtgc acgctgagct
    14221 cggcgatccc gccgcggtag tcggtgcctc cgatcgaggg cagcttggtc agccagacga
    14281 gcaggtagcg ggcggtgacc ggcttgttgg gccgcagcgt gacgttggtg ccggcgtcgt
    14341 cccgctcggc cacccgggtc agctcgtcga cgctgcgcgg acgctggtcg ccggcgtgca
    14401 gcgccacgct cgtgccgcgc ccgaccagct cgaggctcac gctgcccacc gactgggggg
    14461 agcccaggtc gaccagcacc ccgacgccgt ccttgagccc gcccaggttg gcccggttgt
    14521 agtaggtcat cgtcgtccag gcggtcgacg cgtcgccatc gatcgccagg tcgagcttgt
    14581 cgcggttctc ctcgccgttg ccctgcgggt cgaagtcggt cagcgccgac accgggatca
    14641 gcgcacccag cgggggcccg gacgcctcgg tcgtcgcggt cgtcggggtg cccccgccgc
    14701 ggtcggtctg ggagaggctg cccgagacgg tggccacgat ggcccagccg agcaggccga
    14761 cggcgacgac aaggacggcg gcgactgccc agccggccca gctggtgccg gtggtcgggg
    14821 ccggtcttgg ggtcagctcg ggcggcacgt tcgggggtac gacggaggcc gcgacctgtc
    14881 cgtcgtacgc cgtcgggttg tcgcggtggt cgggttcggt gggttggacg tgggtgaggg
    14941 cggcggcgac cgcggccggc gaggagagcg ggctctcccg gtgggccggc tggcccagcg
    15001 cccgcatggt gaccgcgtcg aggccggccg gcacccccgc gcggacctgt cgcggagaca
    15061 ccgcccgccc gtcggcgaca ggtggcggtg gcagcgtcga gccgtgcggt gtctgcctct
    15121 cgcccggcca acggccggtc agggcggcga acagcagtcg cccgcagccg gcggcgtcct
    15181 cgcgggcggc ctcggtctcg gtgatctcgg gcagccctgc caccgccgcg tcgacgcaca
    15241 gcccgaccag ccggacctgg ccggcatcgg tgaccacgac gctggccggg cgcaggcagc
    15301 ggtgccagag ctgttggtgg tgggccgtgg ccagggcctc ggcgacctcg cgggcgaccc
    15361 gggcggcgtc cttgtcgggc agcgggccgg tgttgagcag ctcctcgagc gaggggccgc
    15421 tggcccattc cgacacgacg tagacctgac cctggtcggt cgcgacgtcg aggacccgta
    15481 ggaaccgggg gtcgtggctg gccgcggccg cccgggcggc ggcggtcagc gtggcggcga
    15541 gcgggtcggc ggcggcgacg gtgcgcacgc ccacgtcgcg gcccagggtc tcgtcgaccc
    15601 cgcgccagta ggaggtcgtg gccaggtcga tgacgtggtc ctcgatgcgg tagcgcccgg
    15661 ccagtcgggt ccccgggccc agtgaggacg acggcgggga cgtcggcacg gccctcctcg
    15721 agctggtctg gcggtcctcg cccatgctag gccgcggagc gggtgcacag gtgcgcgcgc
    15781 tagacgcctg gtcggatcgc cccgatgagc ccgtcgagca gcacgcccgt ctcgcgcacc
    15841 ggcatcccgg tgcgaggggc gtcgatcatc cgaccgccgc cgacgtagat gcccacgtgg
    15901 tggatggttg acgggttggt gacgtcggtg gcccagaaca gcaggtcacc gggggccagc
    15961 tcgttcaacg gcaccgcgcg gccggcgaac cactgctggc gtgacgtgcg gggcagcgag
    16021 agcccggcct gccggtaggc ccagccggtc agccctgagc agtcgaacgt ctccggaccg
    16081 accgcgcccc actggtaggg ccgcccgatc atcgcccggg ccgccgcgat cgcttgcggt
    16141 gcccagccgg tgcccggcgg cgcgtcggcc tgcagcagcc ccagctcggc cagccgggtc
    16201 tgcgaggacg cccaggcgag tgcgtgggcg cgggcggcct catcggccac cagggtgcgc
    16261 acccgcgcgt cggcctgctc gagcagcgcc tgcgactggt cgagcagccg ggtgacctcg
    16321 gcggcggcgt cctcggcctg ggcctctagc gccgactgct ggtcggccag ctggcccagc
    16381 tccagcgcga tgtcggcggc ctggtcggtg accgctgcgc ggtcggccac gtcgatcgag
    16441 ctcgcgtcca tgaccgcgtg cacggcggcg atccggctga gcaggtcgcc gatgtcctgc
    16501 ccgtcgagca gtgtcgcgta gaggcccgcg gtgccgcctg ccatgtagat cgcccgcgcg
    16561 cgggcctcga cctctcgtag cgagccgtcg tgggccaccc gcgcggcgtc gagctgacgg
    16621 cgggccagcc cctcccgggt ggccagggtg gcgagctggt cggtgatccc gtcgtagtgc
    16681 tcggtggcca gctcggccgc caccgtcagg gcctcgacct gggtgcgcag cgccgacgcc
    16741 tgctggcggg cggcggccag gtcggggtcg gcgacggccg gaccggtggg tacgacgagc
    16801 aggcttgccg cggtggcgag cgcggccagc gcggtgagga ccgtgcgccc atgggcgccg
    16861 ggtcggatca ccccgtcacg tctcggccgc tggcggtggc ggtgagctga ccccagtcac
    16921 ggatcgggcc gatgcgcgac acgacgatgg tggaggcctc gcggtccagg cgcaggtgca
    16981 cggtgccgtc cgggtcgacc gtgaaccctt cggtggccac ggtgttggcg gcgttgtcct
    17041 cggtggcctg cgccagcgca gcgtcgtacg ccttctgcac gttgtgcccg tgcgcccagg
    17101 cggtgctggc gctgatcgcg gccgccttgg cctgatcggt caaggacacc ttggtccagc
    17161 cgatcgagat gaggtcaaag atcacgagcc ctacgagggc aagcgtgacg acgaccttgg
    17221 ccagccagcc gaggatgatg tcgcctcggt cgtcgttcat gcggtgatgg tcggctcacc
    17281 gggcgacgac cttgaggccg agagcctgct ccctaagggt ggagcttggc cgtggccgaa
    17341 tcatcgaaca atcatcggtt gcctccagat gcttccattt ggcggagctc tccgctagac
    17401 tcgaacacat gttcgataac acgggggacg gtccgggtgc cgacgccatc gggtcgaagc
    17461 caaccgacac cagccacacc gacaccagcg acaccgagcc gctcgcgccg gctgacccgc
    17521 tcgcgccggc tgacccgctc ctgccggttg acccgcccca gccggctgtc tcgacgcccg
    17581 agccaccggc gttgccgggg ttggcggggc ggttgctcgc cgggctggac ccggcctcgg
    17641 cggcggcggt ccgcgcggcg gtcagcca
//
LOCUS       DATIBB010000099         6461 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_53456, whole genome shotgun sequence.
ACCESSION   DATIBB010000099 DATIBB010000000
VERSION     DATIBB010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 6461)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6461)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6461
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53456"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..196
                     /locus_tag="VLC50_03745"
     CDS             <1..196
                     /locus_tag="VLC50_03745"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013115869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HSF26612.1"
                     /translation="ARGIVRAKTGSLTGVSTLAGTLTDADGSLLVFAVLADEVPQTGT
                     LAARRALDAVAAALVACGCR"
     gene            302..1360
                     /locus_tag="VLC50_03750"
     CDS             302..1360
                     /locus_tag="VLC50_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018520454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HSF26613.1"
                     /translation="MVDWDLAVATGSRLVPAGPPVSREEAREAVAQLRAFAVQAQDHV
                     RDYTGLVAPGDGGAVAVIDRPGWIRANAAGFRAVIDPLEERLQERRSRGGALGPGPWD
                     AVGPKVTGVQTGSLLAFLATKVLGQYDLFSTYDESPGRLLLVAPNIVAAEREMGVDPA
                     DFRLWVCLHEETHRVQFGAVPWLREHVLGEIRAYLEATDVDPAAMARRLRDGLGAVVE
                     ALRGSEGTGMLLEAVQTPAQREILDRLTAVMSLLEGHADVVMDGVGPDVIPSVAAIRE
                     RFQRRRQGRGWADQLTRRLLGLDAKMRQYRDGAAFVRQVVDQVGRDGFNAVWSSPAAL
                     PTRAEVLAPASWVARVVG"
     gene            1364..2362
                     /gene="tilS"
                     /locus_tag="VLC50_03755"
     CDS             1364..2362
                     /gene="tilS"
                     /locus_tag="VLC50_03755"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020132506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HSF26614.1"
                     /translation="MGPAPATAVVRRAVRLALDDLPAGTLVLVGCSGGADSLALAAAT
                     AFVAPRQGLRAGGISVDHGLQAGSVERADAVVVIMRGLGLDPVAAVTVAVAGPGGPEA
                     AARQARRAALTEAAARHGAAAVLLGHTRDDQAETVLLGLARGSGARSLSGMAPVAGIW
                     RRPLLGLDRATVRAACAQAGLTAWEDPHNADPVYRRSRIRHDLLPALERQLGPGVAAA
                     LARSGELLRADADALDAWAARERAAAALPDHGEEGETLAVEVLAGLPGAVRRRVLRAV
                     AIEAGAPATDLSATHVRELDRLVTDWHGQGPLHLPGPVLAWRDCGRIRLAARGVTR"
     gene            2377..2937
                     /gene="hpt"
                     /locus_tag="VLC50_03760"
     CDS             2377..2937
                     /gene="hpt"
                     /locus_tag="VLC50_03760"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012854816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HSF26615.1"
                     /translation="MDRTDVNNDLEKVLVTEEQIRARLAELAREIERDYAGQELLLVG
                     VLKGAVMVMADLARALHRHIEMDWMAVSSYGSGTKSSGVVRILKDLDKDISGRHVLVV
                     EDIIDSGLTLSWLLANLGSRKPASVEVCALLRKPEAAQVHIDVRYVGFDIPNEFVVGY
                     GLDYAQRYRNLPFVGTLSPHVYAPGA"
     gene            3137..5134
                     /gene="ftsH"
                     /locus_tag="VLC50_03765"
     CDS             3137..5134
                     /gene="ftsH"
                     /locus_tag="VLC50_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015658789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HSF26616.1"
                     /translation="MDVKRYFRGPIFWVLLAIVGLLVVTQVASSGGADKVDTPKALQA
                     ISDHTAKSVLLVAEPDQQLQITLTDGKKIHASYVEGQQTSIVELLQKNPPPESWDVKV
                     PQQSWLMSLLLTVLPIILIVVVFLFFMNQMQGGGSRVMNFGKSKAKLISKDTPKTTFS
                     DVAGANEAVEELQEIKEFLQEPAKFQAVGAKIPKGVLLYGPPGTGKTLLARAVAGEAG
                     VPFYSISGSDFVEMFVGVGASRVRDLFEQAKANAPAIVFVDEIDAVGRHRGAGLGGGH
                     DEREQTLNQLLVEMDGFDVKGGVILIAATNRPDILDPALLRPGRFDRQIAVERPDLQG
                     RAKILEVHAKGKPISPEVDLMAVARRTPGFTGADLANVLNEAALLTARTDKKLIDATA
                     MDEAIDRVVAGPQKRSRLMNEKEKKITAYHEGGHALVAAALPNTDPVHKITILPRGRA
                     LGYTMVLPDEDKYSTTRNEMLDQLAYMLGGRAAEELVFHDPTTGAANDIEKATGVARS
                     MVTQYGMTERIGAIKLGQDNGEVFLGRDMGHQRDYSEEVAGVVDEEVKRLIESAHQEA
                     WEILVDNREVLDTLVLALLEKETLDKSEVAKIFEPVRRRPSRPAWTGSERRMPSEQGP
                     VMTPKEIELAANGQSPNGDHERSMGVTEPTDVPDTPLPSEG"
     gene            5137..5772
                     /gene="folE"
                     /locus_tag="VLC50_03770"
     CDS             5137..5772
                     /gene="folE"
                     /locus_tag="VLC50_03770"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014137466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HSF26617.1"
                     /translation="MGADPTEIADDVGPVRVDGDRPPQPYDAARVERAVRELLLGIGE
                     DPDREGLRETPARVARAFAEMFSGLRVRAEDVLTTTFDLGHDEMVLVKDIEVFSTCEH
                     HLVPFYGVAHIGYVPSADGRITGLSKVARLVDTFARRPQVQERLTTQIADAFVRILRP
                     RGIIVVVECEHLCMSMRGVRKPGARTVTSAVRGQLRDSATRAEAMSLILGR"
     gene            5769..>6461
                     /locus_tag="VLC50_03775"
     CDS             5769..>6461
                     /locus_tag="VLC50_03775"
                     /inference="COORDINATES: protein motif:HMM:NF028305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF26618.1"
                     /translation="MTRSLAWVPRVAGTACLLIGLMDVASAVTKGMAARVHEIGRLVP
                     GVLSNAAAAATVATGVLLVLLAHALRRRKRRAWRAVVGLLSLSVVLHVVKGLDVEEAL
                     VSLTLLGLLLLARREFYAQGDPRTRWRAVGVLAGLLVADLVIGQVLLLLRESALVGQP
                     TLTERLDEVLMGLIGLDGPVEFRGDTAADLVAFVLFALGSVTILVTTYLVLRPEEPAG
                     RLTEADESRVRAL"
BASE COUNT          865 a         2339 c         2367 g          890 t
ORIGIN      
        1 cgcccgcggc atcgtgcggg ccaagaccgg cagcctgacc ggggtgagca cgctggccgg
       61 cacgctcacc gatgctgacg gctcgctgct cgtgttcgcg gtgctggccg acgaggtgcc
      121 gcagaccggc acgctggccg ctcgtcgggc gctggacgcg gtcgccgcgg cgctggtcgc
      181 ctgcggctgt cgctgagccc gggtctgcac gacgccggat ctgcccgccg gcccgctccc
      241 gtacggtggg gccatgacag gtctcggcgg acccggcgcc agctctcacg agcctcccga
      301 catggtcgac tgggacctcg cggtcgcgac cgggtcccgg ctcgtcccgg ccggcccgcc
      361 ggtcagccgg gaggaggccc gcgaggcggt cgcccagctg cgcgcgttcg ccgtgcaggc
      421 ccaggaccat gtgcgcgact acaccgggtt ggtcgcgccg ggcgacggtg gcgcggtcgc
      481 ggtcatcgac cggccgggct ggatccgggc caacgcggcc gggtttcgcg ccgtgatcga
      541 cccgttggag gagcgcctgc aggagcgccg ctcccgcggt ggggcgctcg gccccgggcc
      601 gtgggacgcc gtgggcccca aggtgaccgg cgtgcagacc ggctcgctgc tggcgttcct
      661 ggccaccaag gtgctcggtc agtacgactt gttctcgacg tacgacgagt cgcccggccg
      721 gctgctgctg gtcgcgccca acatcgtcgc cgccgagcgc gagatgggcg tggaccccgc
      781 cgacttccgg ctgtgggtct gcctgcacga ggagacccac cgggtgcagt tcggcgcggt
      841 gccctggctg cgcgagcacg tcctgggcga gatccgggcc tacctcgagg ccaccgacgt
      901 cgaccccgcg gcgatggccc gacggctgcg cgacggcctc ggcgcggtgg tcgaggcgct
      961 gcgcggctcg gaaggcaccg ggatgctgct cgaggcggtg cagacgccgg cgcagcggga
     1021 gatcctcgac cggctgaccg cggtgatgtc gctgctcgag ggccacgccg acgtcgtgat
     1081 ggacggggtg ggccccgacg tcatcccgag cgtcgcggcc atccgtgagc ggttccagcg
     1141 ccgccgccag ggccggggct gggccgacca gctgacccgc cggctgctcg ggctcgacgc
     1201 caagatgcgc cagtaccgcg acggcgccgc cttcgtgcgc caggtcgtcg accaggtcgg
     1261 ccgcgatggg ttcaacgcgg tctggtcctc gccggcggcg ctgcccaccc gcgccgaggt
     1321 gctcgcgccg gcgagctggg tcgcccgagt cgtcggctga ccgatgggtc cggcaccggc
     1381 cacggccgtc gtacggcggg cggtgcggct cgcgctcgac gacctgccgg ccgggaccct
     1441 cgtgctggtc gggtgcagcg gcggggccga ctcgctcgcc ctggcggccg cgaccgcctt
     1501 cgtcgcgccg cggcaaggcc tgcgggccgg cgggatcagc gtcgaccacg ggctgcaggc
     1561 cggctcggtc gagcgggccg acgcggtggt cgtgatcatg cgcgggctcg ggctcgaccc
     1621 cgtcgcggcg gtcaccgtcg ccgtcgccgg gccgggcggg ccggaggcgg cggcccggca
     1681 ggcgcgccgg gccgcactga ccgaggccgc cgcccggcac ggcgcggccg ccgtcctgct
     1741 cggacacacc cgcgacgacc aggccgagac ggtgctgctg ggcctggccc gcggctcggg
     1801 ggcccgctcg ctgtcgggca tggcgccggt ggccgggatc tggcgccggc ccctgctcgg
     1861 cctggaccgg gccaccgtgc gggccgcctg cgcgcaggcc gggctcaccg cctgggagga
     1921 cccgcacaac gccgacccgg tctaccggcg ctcccggatc cgccacgacc tgctgcccgc
     1981 cctcgagcgc cagctggggc ccggcgtcgc ggccgccctg gcccgctccg gcgagctgct
     2041 gcgcgccgac gccgacgccc tcgacgcgtg ggcagcccgg gagcgggccg ctgcggcact
     2101 gcctgatcac ggggaagagg gggagacgct ggccgtcgag gtgctggccg gccttcccgg
     2161 cgccgtacgg cgaagggtgc tgcgcgccgt cgcgatcgag gccggcgccc cggccaccga
     2221 cctgtcggcc acccacgtgc gggagctcga ccggctggtc accgactggc acggccaggg
     2281 gccgctgcac ctacccgggc cggtcctggc ctggcgcgac tgtgggagga tccgcctcgc
     2341 cgcacggggt gtcacgcgct agggaggatc ggccgggtgg atcgcaccga cgtcaacaac
     2401 gacctcgaga aggtgctcgt caccgaggag cagatccgcg cccggctggc cgagctggcc
     2461 cgcgagatcg agcgcgacta cgccgggcag gagctgctgc tcgtcggcgt gctcaagggt
     2521 gcggtgatgg tcatggccga cctggcccgg gccctgcacc ggcacatcga gatggactgg
     2581 atggccgtct cgtcctacgg cagcggcacc aagtcctccg gcgtcgtccg gatcctcaag
     2641 gacctcgaca aggacatcag cggacggcac gtgctggtgg tcgaggacat catcgactcc
     2701 ggcctgacgc tgtcgtggct gctggccaac ctcggctcgc gcaagccggc ctcggtcgag
     2761 gtgtgcgcgc tgctgcgcaa gcccgaggcg gcccaggtgc acatcgacgt ccgctatgtc
     2821 gggttcgaca tccccaacga gttcgtcgtg ggctacggcc tcgactacgc ccagcgctac
     2881 cgcaacctgc cgttcgtcgg caccctgtcg ccgcacgtct acgccccggg ggcgtaagcc
     2941 cggacgggtt cgccaagcgg aaccctcacg ccgtaccgta cgttgacacg ctcgacggtt
     3001 tcctcgcggc gcgacgcgtc acggcggcgt cgcggcggtg ttgcgttcgg gcaccgggcc
     3061 gccgccgtgc cccggtgtac cgtcgttgtt ccgacaccac gtagcaggag gggacgaggg
     3121 cgcccgcccc gcaccaatgg acgtcaagcg ctacttccgc gggccgatct tctgggtcct
     3181 gctcgccatc gtcggcctgc tcgtggtcac ccaggtcgcc tcctccggcg gcgccgacaa
     3241 ggttgacacc cccaaggcgc tgcaggcgat cagcgaccac accgccaagt cggtgctgct
     3301 ggtggccgag cccgaccagc agctgcagat cacgctgacc gacggcaaga agatccacgc
     3361 cagctacgtc gagggccagc agacctcgat cgtcgagctg ctgcagaaga acccgccgcc
     3421 ggagtcgtgg gacgtcaagg tcccgcagca gtcgtggctg atgtcgttgc tgctcaccgt
     3481 gctgccgatc atcctcatcg tcgtcgtctt cctgttcttc atgaaccaga tgcagggcgg
     3541 cggctcgcgg gtcatgaact tcggcaagtc caaggccaag ctcatctcca aggacacccc
     3601 caagacgacg ttcagcgacg tcgccggtgc caacgaggcg gtcgaggagc tgcaggagat
     3661 caaggagttc ctccaggagc cggccaagtt ccaggcggtc ggcgccaaga tccccaaggg
     3721 tgtgctgctc tacggcccgc ctggcaccgg caagaccctg cttgcccggg cggtcgcagg
     3781 cgaggccggg gtgccgttct actccatctc cgggtccgac ttcgtcgaga tgttcgtcgg
     3841 cgtcggcgcc tcccgggtgc gcgacctgtt cgagcaggcc aaggccaacg cgccggcgat
     3901 cgtgttcgtc gacgagatcg acgcggtcgg ccgccaccgc ggggccggcc tcggcggcgg
     3961 gcacgacgag cgcgagcaga ccctcaacca gctgctcgtc gagatggacg gcttcgacgt
     4021 caagggcggg gtcatcctca tcgcggccac caaccggccc gacatcctcg acccggcgct
     4081 gctgcggccc ggtcggttcg accgccagat cgcggtcgag cgccccgacc tgcagggccg
     4141 ggccaagatc ctcgaggtgc atgccaaggg caagccgatc tcccccgagg tcgacctgat
     4201 ggcggtggcc cgtcgtactc ccgggttcac cggcgccgac ctggccaacg tgctcaacga
     4261 ggccgcgctg ctgaccgcgc gcaccgacaa gaagctcatc gacgcgaccg cgatggacga
     4321 ggcgatcgac cgggtcgtgg ccggtccgca gaagcgctcg cggctgatga acgagaagga
     4381 gaagaagatc accgcctacc acgagggcgg acacgcgctg gtggcggcgg cactgcccaa
     4441 caccgacccg gtgcacaaga tcacgatcct gccgcgcggg cgggcgctcg gttacacgat
     4501 ggtgctgccc gacgaggaca agtactccac cacccgcaac gagatgctcg accagctcgc
     4561 ctacatgctc ggcgggcggg ccgccgagga gctggtcttc cacgacccca cgaccggggc
     4621 ggccaacgac atcgagaagg ccaccggggt ggcgcgctcg atggtcacgc agtacggcat
     4681 gaccgagcgg atcggggcga tcaagctcgg ccaggacaac ggcgaggtgt tcctcggccg
     4741 tgacatgggc caccagcgcg actactccga ggaggtcgcc ggggtggtcg acgaggaggt
     4801 caagcgcctc atcgagtcgg ctcaccagga ggcctgggag atcctggtcg acaaccgcga
     4861 ggtgctcgac accctggtgc tggccctgct cgagaaggag acgctcgaca agagcgaggt
     4921 cgccaagatc ttcgagcccg tacgccggcg cccctcgcgc ccggcgtgga ccggctccga
     4981 gcggcggatg ccctcggagc agggcccggt gatgacgccc aaggagatcg agctcgccgc
     5041 caacgggcag tcgccaaacg gcgaccatga gcgcagcatg ggcgtgaccg agccgaccga
     5101 cgtgcccgac acgccattgc ccagcgaggg ctgaccatgg gggccgaccc caccgagatc
     5161 gccgacgacg tcggtccggt gcgggtcgac ggcgatcgac caccgcagcc ctacgacgcg
     5221 gcccgcgtcg agcgcgccgt acgtgagctg ctcctcggca tcggcgagga ccccgaccgt
     5281 gaggggctgc gcgagacccc ggcccgggtg gctcgggcgt tcgcggagat gttcagcggg
     5341 ctgcgcgtgc gcgccgagga tgttctcacc acgacgttcg acctcggcca cgacgagatg
     5401 gtgctggtca aggacatcga ggtgttcagc acctgcgagc accacctggt gccgttctac
     5461 ggcgtcgcgc acatcggcta cgtcccgtcg gccgacggac gcatcaccgg gctgtccaag
     5521 gtggcccggc tcgtcgacac cttcgcccgc cggccgcagg tgcaggagcg gctgaccacc
     5581 cagatcgccg acgcgttcgt gcgcatcctg cgcccgcggg ggatcatcgt cgtggtcgag
     5641 tgcgagcacc tgtgcatgtc gatgcggggg gtgcgcaagc ccggggcccg caccgtcacc
     5701 tccgccgtac gcggccagct gcgcgactcc gcgacccggg ctgaggcgat gagcctcatc
     5761 ctcggccggt gacccgctcg ctggcctggg tgccccgggt cgcggggaca gcgtgcttgc
     5821 tcatcgggct gatggacgtc gcctctgccg tgaccaaggg gatggcggcc cgggtgcacg
     5881 agatcggccg cctggtgccc ggcgtgctga gcaacgccgc cgccgcggcc accgtcgcga
     5941 ccggggtgct gctcgtgctc ctggcccacg ccctgcgccg gcgcaagcgc cgggcctggc
     6001 gagcggtcgt ggggctgctg tccctcagcg tggtcctgca cgtcgtcaag gggctcgacg
     6061 tcgaggaggc gctggtctcc ctgaccttgc tgggcctgct gctgctcgct cgccgcgagt
     6121 tctacgcgca gggcgacccg cggacccggt ggcgtgcggt cggtgtgctc gccgggctcc
     6181 tggtggccga cctcgtcatc gggcaggtgc tgctgctcct gcgtgagagc gcgctcgtcg
     6241 gccagcccac gttgaccgag cgcctggacg aggtgctgat gggcctgatc ggtctcgacg
     6301 ggccggtgga gttccgaggc gacaccgccg ctgatctcgt ggccttcgtc ctgttcgccc
     6361 tggggtcggt gacgatcctg gtcaccacct acctcgtgct gcgcccggag gagccggccg
     6421 gccggctcac cgaggccgac gagagccggg tccgggctct c
//
LOCUS       DATIBB010000100         4075 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_1499770, whole genome shotgun sequence.
ACCESSION   DATIBB010000100 DATIBB010000000
VERSION     DATIBB010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 4075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4075
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1499770"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..294
                     /locus_tag="VLC50_03780"
                     /pseudo
     CDS             1..294
                     /locus_tag="VLC50_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005247561.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
     gene            298..918
                     /locus_tag="VLC50_03785"
     CDS             298..918
                     /locus_tag="VLC50_03785"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSF26619.1"
                     /translation="MVHAFSRRRAPATHVAAAADGDAARLEGAARARLERRANDRGAL
                     SALTEALVAQRRFAEAATASRRLVDHNDRTYSGGGEELEAHARVLLLGVESGQLCLDE
                     VREELDTTLDRAQRVVGSGAVLAGTHALHALVTGRYVEAEAAGNEALRWRPSARERAD
                     ALLTLARVYAATDRASEAETTLAEARTAWPDNPRLAQPEAVNSSRA"
     gene            complement(885..1304)
                     /locus_tag="VLC50_03790"
     CDS             complement(885..1304)
                     /locus_tag="VLC50_03790"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015658066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(deoxy)nucleoside triphosphate
                     pyrophosphohydrolase"
                     /protein_id="HSF26620.1"
                     /translation="MATPLVVVGAALFVRGRMLAARRVAPPALAGGWELPGGKVDPGE
                     TDEQALRRELAEELGVRVKLLDRVPGEWPLGETAVLRVWSAVLVHGEPDPAPLEDHDA
                     LRWLGLDELDAVAWLDADRPAVEAVRRHARELFTASG"
     gene            1384..1683
                     /locus_tag="VLC50_03795"
     CDS             1384..1683
                     /locus_tag="VLC50_03795"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HSF26621.1"
                     /translation="MPRLLDDEELTRQLRDLPAWTRDGAVLRRTVEAPDFPAAIAIVN
                     DLADVAEAMNHHPDIDIRWRTLHLALSTHSAGGITQYDVELAHRIEEITRDRGAS"
     gene            complement(1738..2298)
                     /locus_tag="VLC50_03800"
     CDS             complement(1738..2298)
                     /locus_tag="VLC50_03800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF26622.1"
                     /translation="MSAADGRRRVRSRQLVGVLAGLTVLVLNSPGAADGAVVAAPAFA
                     GVAALTGSSAELAGVVFEGVATVQTATGTVETIKLTTTAAAITDLRLRPPCTPISGLG
                     AGMTTESTTPGQSISRAPAGMTVYATALTASTPTGSVTWTAANPPPARQLGDLSLAEL
                     SVQLVSLRAPEIVLPGLHQASLYCAS"
     gene            complement(2295..2756)
                     /locus_tag="VLC50_03805"
     CDS             complement(2295..2756)
                     /locus_tag="VLC50_03805"
                     /inference="COORDINATES: protein motif:HMM:NF039352.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6114 domain-containing protein"
                     /protein_id="HSF26623.1"
                     /translation="MSTEDGSSTEEEPRDGEPLRDVPERPSLRRRFRAWRRSRPFWGG
                     LFTMLGGLEIATIPLTAYRIILVSHSVVVAAFVGVVIAILGLLLWMTPSHNKLYGLLV
                     VLLGVVSFVTSNVGGFLLGGLLSVIGGALGFAWVVVKPAVPISDAPEREWA"
     gene            complement(2778..3524)
                     /locus_tag="VLC50_03810"
     CDS             complement(2778..3524)
                     /locus_tag="VLC50_03810"
                     /inference="COORDINATES: protein motif:HMM:NF039694.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6230 family protein"
                     /protein_id="HSF26624.1"
                     /translation="MTSSSESPGAVEGRTRWRRFGLAAGVGLGAVGLVGYLGATGALA
                     LTFAFSGIPFTLSADSLGGTGFVQYAFPDRLASNAGASALEGGAGRLIDGSGSINNTA
                     TVNGTTYASDTVTQLRNATISGLSQTICAPTPLGNAIRVTIAGSGDTTAGNLVIQAPA
                     LTADSAKFSNIIIGESVGSALQGQGFGANNSFTDPYGALSGAPIGGSFAQGADSVTLT
                     NINQIGVGTQAGSFNISGLKLHAEFVSSCG"
     gene            complement(3738..>4075)
                     /locus_tag="VLC50_03815"
     CDS             complement(3738..>4075)
                     /locus_tag="VLC50_03815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF26625.1"
                     /translation="GRVLVAHHARLELGFLTRAAEQAYGARCPLTAVDTLAVQHDLVI
                     GRYGEIRPGALRLDEARRQMGLPRYLAHRALTDAIAAAELLLAQIAEIEHRLGREPAL
                     EDLSPAHRR"
BASE COUNT          636 a         1448 c         1435 g          556 t
ORIGIN      
        1 cgcaagtgca tcgagtgctt cctgtgccag gacgtctgcc acgtcatccg tgaccacgag
       61 gacaacaaga agtccttcgc cgggccgcgg ttcttcatca ggctggccga gctcgacatg
      121 cacccgctcg acaccgtcga ccggcgcgcg ctggcccagg acagccacgg cctgggcatg
      181 tgcaacatca ccaagtgctg caccgaggtc tgcccggaag gcatcaagat caccgacaac
      241 gcgatcatcc cgatgaagga gcgggtggcc gaccgccgct acgacccgct catgcggatg
      301 gtgcacgcct tctcgcgccg gcgagccccc gccacccacg tggccgccgc ggccgacggg
      361 gacgcggctc ggctcgaggg ggcggcgcgg gcccggctcg agcgacgcgc caatgaccgg
      421 ggggccctct cggccctcac cgaggccctg gtcgcccagc gccgtttcgc cgaggccgcc
      481 acggcctcgc gccggctcgt cgaccacaac gaccgcacct actccggcgg cggcgaggag
      541 ctcgaggcgc acgcgcgggt gctgctgctc ggggtggaat cgggccagct ctgcctcgac
      601 gaggtgcgcg aggagctgga cacgacgctg gaccgggctc aacgcgtggt gggcagcggc
      661 gcggtgctgg cggggaccca cgccttgcat gcgctggtga ccgggcgcta cgtcgaggcg
      721 gaggccgcgg gcaacgaggc gctgcggtgg cgcccgtcag cgcgagaacg cgccgacgcg
      781 ctgctcacgc tggccagggt ctacgccgcc accgaccggg ccagcgaggc cgagacgacg
      841 ttggcggagg cgcgcacggc ctggccggac aacccgcggc tggctcagcc ggaggcggtg
      901 aacagctcgc gggcatgacg tcgtacggcc tcgaccgctg gccggtcggc gtcgagccag
      961 gccaccgcgt cgagctcgtc caggcccagc cagcgcaggg cgtcgtggtc ctcgagcggc
     1021 gccgggtcgg gctcaccgtg cacgaggacc gcgctccaca cccgcaggac cgcggtctcg
     1081 ccgagcggcc actcccccgg cacccggtcg agcagcttga cccgcacacc gagctcctcg
     1141 gccagctcgc ggcgcagcgc ctgctcgtcg gtctcgccgg ggtcgacctt gccgccgggc
     1201 agctcccacc caccggccag tgcgggcggg gcgacgcgcc gggcggcgag catccggccg
     1261 cggacgaaca gggccgcgcc gacgaccacc agcggagtcg ccacgccctc acctccatcc
     1321 gccaaccgat ctcgggcacc gtagcgggtt gacccggcgt gatcaccgcg aaagggtcag
     1381 ggcgtgcctc gactgctgga cgacgaagag ctcacccgtc agctgcgtga cctgcccgcg
     1441 tggacccgcg acggcgcggt cttgcgccgc accgtcgagg cgcctgactt ccccgctgcg
     1501 atcgcgatcg tcaacgacct ggccgatgtg gccgaggcga tgaaccacca ccccgacatc
     1561 gacatccgct ggcgcacgct gcacctggcg ctgtcgaccc actcggccgg tgggatcacg
     1621 cagtacgacg tcgagctggc gcaccgcatc gaggagatca cccgcgaccg cggcgccagc
     1681 tgacccacgg cgttgtgatc gggctcttgg gtctggctcg caccgctgac gtgcggacta
     1741 gctcgcgcag tagagactcg cctggtgcag gccgggcagg acaatctcgg gtgcgcgcag
     1801 gctcaccagc tgcacggaca gctcggccag cgagaggtca ccgagctgtc tggccggtgg
     1861 cgggttggct gcggtccagg tgaccgatcc cgtgggcgtg ctcgccgtga gcgcggtggc
     1921 atagaccgtc atgcccgccg gcgccctcga gatgctctgg cccggcgtcg tcgactcggt
     1981 ggtcatccct gctccgagcc cgctgatcgg cgtgcagggc ggacgcagcc gcaggtcggt
     2041 gatagccgca gcagtggtcg tgagcttgat cgtctcgacg gtccccgtcg cggtctgcac
     2101 cgtggccacg ccttcgaaga ccacaccggc gagctctgcc gaggagccgg tgagggcggc
     2161 cactccagca aacgcggggg cggccaccac ggcaccgtcg gctgcgcccg gagagttgag
     2221 gaccagcacc gtcagaccgg ccagcacccc gacgagctga cgactcctga cccgtcgccg
     2281 gccgtcggca gcgctcatgc ccactcgcgt tcgggcgcgt cggagatcgg caccgccggt
     2341 ttcacgacga cccatgcgaa cccgagcgcg cccccgatga ccgacagcag acccccgagg
     2401 aggaagcccc cgacgttgct ggtcacgaac gagacgacgc cgagcagcac gacgagcagc
     2461 ccatagagct tgttgtggct gggggtcatc cacaggagca ggcccaagat cgcgatgacc
     2521 acgcccacga acgccgcgac cacgaccgag tgcgagacca ggatgatccg ataggcggtg
     2581 agcgggatcg tcgcgatctc caggccgccg agcatggtga acagcccgcc ccagaacggc
     2641 cgcgaacggc gccaggcgcg gaaccggcgt cgaagcgacg gccgctccgg gacgtcccgg
     2701 agcggctcgc cgtctctcgg ttcttcctcc gtggaggaac cgtcctcagt cgacatgaga
     2761 gtgcaagggc tttcgcgtca tccgcagctg ctgacgaact cggcgtgcag cttgaggccg
     2821 ctgatgttga agctgccggc ctgggtgccc acaccgatct ggttgatgtt ggtcagggtg
     2881 acgctgtcag caccctgcgc gaagctgccg ccgatcgggg ctccggagag cgcgccgtac
     2941 gggtcggtga aggagttgtt ggccccgaag ccctggccct gcagggccga gccgaccgac
     3001 tccccgatga tgatgttgct gaatttcgca ctgtcggcgg tcagcgcggg ggcctggatc
     3061 accaggttgc ctgccgtcgt gtcgccagac ccggcgatgg tcacgcggat ggcattgccc
     3121 aacggggtcg gggcgcaaat cgtctgggac agcccgctga tcgtcgcgtt gcgcagctgg
     3181 gtcaccgtgt cggatgcgta agtggtgccg ttgacggtgg cagtgttgtt gatgctgccg
     3241 gatccgtcga tgagccggcc ggcgccgcct tccagggcgc tcgccccggc gttgctggcc
     3301 aaccggtcgg ggaatgcgta ctggacgaag ccggtgccgc ccaggctgtc ggccgacaac
     3361 gtgaaaggga tgccggagaa ggcgaaggtc aaagccagtg cccccgtggc cccgagatag
     3421 ccgacgagcc cgacggcccc caggccgact cccgcagcca gaccgaaacg acgccaccgg
     3481 gtgcggccct cgaccgcgcc agggctttcc gaagaactgg tcatctgctc ccccccaagc
     3541 agtgatccgg tgcacggcgg gcggccgtgc accctccacc cctggcatag acgatcagcg
     3601 gcgatcgcgt agggcgaatg ttcaaacagg ccgtcgaatc agcctttggg tcgcctgact
     3661 atcgtttcta acggtgcatt cgacttcata aggactcttt gcccttatca gcaagcacag
     3721 caaaatctgg tgcagtctca tcgacggtgg gccggggaca ggtcttccag cgccggctcg
     3781 cgaccgagcc ggtgctcgat ctcggcgatc tgggccagca gcagctcggc cgcggcgatg
     3841 gcatcggtca gggcccggtg ggcgaggtag cgcggcagcc ccatctgccg gcgggcctcg
     3901 tcgagacgca gcgcgccggg ccggatctcg ccgtaccggc cgatcaccag atcgtgctgc
     3961 acagccaggg tgtcgaccgc ggtgagtggg caccgggcgc cgtacgcctg ctccgccgcc
     4021 cgggtcagga agcccagctc gagccgggcg tggtgggcga ccagcacgcg cccgt
//
LOCUS       DATIBB010000101        19154 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_448718, whole genome shotgun sequence.
ACCESSION   DATIBB010000101 DATIBB010000000
VERSION     DATIBB010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 19154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19154
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_448718"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1295
                     /gene="mtrB"
                     /locus_tag="VLC50_03820"
     CDS             <1..1295
                     /gene="mtrB"
                     /locus_tag="VLC50_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018891533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MtrAB system histidine kinase MtrB"
                     /protein_id="HSF26626.1"
                     /translation="QEPPRVSLDVASNEVDVTSIPDVLREKVRTEPTQQWRFVEQRYR
                     DGRVEPGLAVGARVVVPEAGTYELYYLFPLTHEHETLSLVRRTLTFAGLALILLVGAI
                     AWLVTRQVVTPVRAAARIAERLAAGHLEERMRAHGEDDLARLAASFNRMATNLQRQIR
                     RLEELSRVQRRFVSDVSHELRTPLTTVRMAADVIHEMREDFDPAVRRSAELLQAQLDR
                     FEALLADLLEISRFDAGAAVLDTEPIDLRDLATRVMEANEPLAARLGSTVTLVAPPKP
                     CVAEVDSRRIERIVRNLLVNALEHGEGRPVVVRVAADEAAVAIAVRDHGVGLKPGEAA
                     LVFNRFWRADPARARTTGGTGLGLSIALEDAHLHSGWLQAWGDPGDGSQFRLTLPRLA
                     GADIVDSPIPLEPADAARGRAAVSAGASTPVPPSGTAS"
     gene            1292..3010
                     /locus_tag="VLC50_03825"
     CDS             1292..3010
                     /locus_tag="VLC50_03825"
                     /inference="COORDINATES: protein motif:HMM:NF022113.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpqB family beta-propeller domain-containing
                     protein"
                     /protein_id="HSF26627.1"
                     /translation="MTTRRRTTKAVLALLSAVVLAAGCAQIPTSGPVHSGRELGVNQP
                     DSYVRALPAPPRLGATPVEIVRGFLDASASFEDDFAVARMFLEPDTRESWDPGKGITI
                     YGTGEGFSLAPSRGGGVGMSAPTVATISPEGVRRPADGADPVAVTFGLVRVQGQWRIS
                     SLPDGLLLTDIDVSRSYRAVNIYFLDPSQQILVPDPVFVPKSPGTSTAVVKRLLRGPS
                     TWLDPGVTSAFPVGTALSGSVPVENGVALVDLNDVALSASPKAREALSAQLVWTLSQV
                     EDVQSVRITVGGTPYSVRGAGEVQPRESWQDFDPGALPSDAVPHIVRDNKLGILDDDT
                     FTAVPGPLGSGKLPLVDPALTPRADLVAALSEDRTSLLVGSVRSELKARLTGGDLTAP
                     SWDVVPTTWVADRSAPGALWMVPRSGEPVRVKVDDIADRPITAVRVARDGTRIALVAG
                     AGEAGRLYVGAIARGDGHDNPARVEGLRLLSPAITSITDVDWAEADQLAVLGRVGKAD
                     PAIYLVDLDGQAVTATPAIPTVVGLAAAPWPLPMIAATGDGQIYRLVGRRWVPVGEGI
                     APFYPG"
     gene            3106..3918
                     /locus_tag="VLC50_03830"
     CDS             3106..3918
                     /locus_tag="VLC50_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013131030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HSF26628.1"
                     /translation="MDALDPSATAAGTVRRVPARLLATALDLVLPQHCLGCQAPGAPV
                     CHDCARPLRASPRRVAPTPTPVGFPPTWAVADYDGLVRKAVVAHKEEGRRDLAPLLSA
                     ALAASLRAAVDDAFGDTNPAGGPEIGARHRPGRPIPVLVVPIPSRPSSRRQRGDDPTG
                     RLARRALAALAGSRPGLHRVGLLRHARGVADQAGLDAAARAANLAGALAARPLPRGVG
                     RGPLVVIVDDVLTSGATLTEAARALHAGGAGLVVAAVVAATVRRLVPGGRDD"
     gene            4237..4902
                     /gene="raiA"
                     /locus_tag="VLC50_03835"
     CDS             4237..4902
                     /gene="raiA"
                     /locus_tag="VLC50_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012854352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HSF26629.1"
                     /translation="MEIVVKGRRTEVSQRFRRHVIEKLAKVERLAPRVMRVDVELSSE
                     RNPRRAEQSQRVELTCRSKGPVIRAEAAADDLYAALDLAFARLEQRLRRAADRRRVHH
                     GSRTPVSVAAATSTLASATSAGQGAAGPAAEAAAPPLGAADEHLAPLVGVNGEGPMVI
                     REKVHAAVPMSLEQALYEMELVGHDFFLYVDARSAAPSVVYRRRGYDYGVIRLEVAER
                     VED"
     gene            5008..5703
                     /locus_tag="VLC50_03840"
     CDS             5008..5703
                     /locus_tag="VLC50_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019629173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HSF26630.1"
                     /translation="MDVETTGGPIPAEPIRVLIVDDHALFRRGLEMVLAQETDIEVIG
                     EAADGSEALVMSRELAPDIVLMDVRMPRRSGIEACTAIKDAVPSAKIVMLTISDEEAD
                     LYEAIKAGASGYLLKEISIDEVADAIRAVHSGQSLISPAMASKLLTEFATMIKRSDER
                     AQVPAPRLTDRELEVLKLVARGLNNRDIAKELFISENTVKNHVRNILEKLQLHSRMEA
                     VVYAVREKLLEIT"
     gene            complement(5696..6706)
                     /locus_tag="VLC50_03845"
     CDS             complement(5696..6706)
                     /locus_tag="VLC50_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018956595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HSF26631.1"
                     /translation="MSATTDQEKTSEQQSPEQEGGGTFAKLTDAVRDYVTAKGSGTVK
                     GLGDKLSGLTHSLEKTADTGGFKAAAAGKTAEKMAEGESAPKAALTGVASGVKKKVGG
                     IFGGGDKDGGGKKKLPTSIVEDIFVGVPVDVAYNQWTQFQELPSYAKGPQSVEQKDDA
                     TSRWVAKIGLSTREWTTTIQEQVPERRIAWSSEGDKGTVDGVITFHPLADDLTLLLLV
                     LEYQPKGFVEWMGNRWRVVGRRARLDLKHYRRFVMMSGDATGAWRGEIREGEVVPAGD
                     EADQDGRAQPKERAEKTQQQTDEPERPGQEQKGQEGRQVPQQGEGQEAGAEEEKATSP
                     LM"
     gene            complement(6703..7269)
                     /locus_tag="VLC50_03850"
     CDS             complement(6703..7269)
                     /locus_tag="VLC50_03850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF26632.1"
                     /translation="MNDTPNPNMIKMGAATAVGYVLGRTKKGRAGLTLALWLAGGNKY
                     TPKNLVRTGVLTVARHPETAALVSQLRGSLAQAGRQAMRATVEARAASLTTSLQQRTE
                     ALSKVGEQGAGAAGKGGKQQDEGPQEEREPAGEQGEQDQRGQQGEQDEQEPEGRGEDT
                     SRQSQRPSGRTPAQRTPQKSSSTEGVKG"
     gene            complement(7351..8097)
                     /locus_tag="VLC50_03855"
     CDS             complement(7351..8097)
                     /locus_tag="VLC50_03855"
                     /inference="COORDINATES: protein motif:HMM:NF018129.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031411 - gas vesicle [Evidence IEA];
                     GO_process: GO:0031412 - gas vesicle organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GvpL/GvpF family gas vesicle protein"
                     /protein_id="HSF26633.1"
                     /translation="MTLLVHAIIRAEDGQLAGLAQHGARVVADDVVAAVVSDVAGEID
                     ERDAAPHLELLSALAETIPALPVSFATVAPDDEAVRRELLRPAGEVLRRQVESVADLI
                     EVRLRVHFDEQTSLRAVVAEDEQLRRLSQASRAEAPGGSALLTLGEQVVTRLTDRHAS
                     MFWALVAPVVEIADRVQVLAQDHERQAAAFLLVRGHLPDMDAAVARMREALPANARLD
                     YVGPLPPLAFLADVLAETAADQQRSSGWGW"
     gene            complement(8094..8570)
                     /locus_tag="VLC50_03860"
     CDS             complement(8094..8570)
                     /locus_tag="VLC50_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006540481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gas vesicle structural protein GvpA"
                     /protein_id="HSF26634.1"
                     /translation="MTVTHGSVYRTPRPSSLADVLDVILDKGIVIDAYVRVAVLGIEL
                     LTIDARIVVASVDTYLRFAEAVNRLDLQPQKQVAGLPGLMEEVTEGGAEHKTKGALRG
                     AKETAQDVISSFKGDQGEGENEPRESAEQPSPVPAQTGASRSAPASRQPPDRGGQG"
     gene            complement(8567..8893)
                     /locus_tag="VLC50_03865"
     CDS             complement(8567..8893)
                     /locus_tag="VLC50_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005463874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gas vesicle protein K"
                     /protein_id="HSF26635.1"
                     /translation="MTASRASSERIDGSGEDVGRSLGRLVVALLEVVRQVIERQALRR
                     VDAGGLSSEEVERLGRALMELERTFDELADTFGVTPEDLYLPVDLTALLPDDTVDHLP
                     RRRTPS"
     gene            complement(8890..9114)
                     /gene="gvpJ"
                     /locus_tag="VLC50_03870"
     CDS             complement(8890..9114)
                     /gene="gvpJ"
                     /locus_tag="VLC50_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018503865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0012506 - vesicle membrane [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gas vesicle protein GvpJ"
                     /protein_id="HSF26636.1"
                     /translation="MPTAERPGILGGTTSSGTSLVDLLDRVVQRGAFVSGDVTICLAG
                     IDLVRLDLRLLLTGITGIEASDTRDRRAAS"
     gene            complement(9101..10126)
                     /locus_tag="VLC50_03875"
     CDS             complement(9101..10126)
                     /locus_tag="VLC50_03875"
                     /inference="COORDINATES: protein motif:HMM:NF018129.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GvpL/GvpF family gas vesicle protein"
                     /protein_id="HSF26637.1"
                     /translation="MPTADRNELARLVSDRAAGVAQEAMRIAHDTAVQRLSSLLADAL
                     VTAALDTLAPAEPSTPARTRDGPPAVRVPAARTVDGSSRPDEGLYCYGITWAGTAMPP
                     ARSLTGQGRATLLPDGDLALVVSRERLAGLDVDEDDLSEEGDLARLVRGHDAVVRAVF
                     EHAAVLPLRFATVVEDEAGAQQLLRAHADVARERLRHVEGATEWGLRLVRAPATSAGG
                     VEPGGRPDGTGPSARAGTEYLARRREALRAHQADNEIAALAADKVAAELDYTVDVIHR
                     GGSAGSALLLDAAYLVATATEARFRADTDRLARELIGHGLHLELTGPWPPYSFATLAQ
                     EVTDAHS"
     gene            complement(10113..10496)
                     /locus_tag="VLC50_03880"
     CDS             complement(10113..10496)
                     /locus_tag="VLC50_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007449987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gas vesicle protein"
                     /protein_id="HSF26638.1"
                     /translation="MTVQSSPPVQGALRSQPSSLADVLERVLDKGIVIAGDVVVNVLD
                     LELLTLRLRLFVSSADTARELGMDWWTTDPFFSSVAARDERRELRDRVAQLEALLQQH
                     GIEAAPEGPDRPETDATAETGHADR"
     gene            complement(10493..10786)
                     /locus_tag="VLC50_03885"
     CDS             complement(10493..10786)
                     /locus_tag="VLC50_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018503862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gas vesicle protein"
                     /protein_id="HSF26639.1"
                     /translation="MNQGQDPSPTPGSDGVGAIVRSALAAFDEVTGHAPEDVSGVRKE
                     GDGWSVLVEVLELERIPDTTSVMATYRVDLDAQGALTGFERLRRYTRGAVDRS"
     gene            complement(10999..12471)
                     /gene="ligD"
                     /locus_tag="VLC50_03890"
     CDS             complement(10999..12471)
                     /gene="ligD"
                     /locus_tag="VLC50_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014740105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HSF26640.1"
                     /translation="MSPRKPPAAPPDDDALATYRSKRDPGRTPEPVPGVGPLPRGDDD
                     TFVIQEHHARRLHWDVRLERDGVLVSWAVPRGLPDDPKRNHLAVHTEDHPLEYAGFAG
                     WIPKGEYGGGQVIIWDRGTYECEKWTDDEVKVVLHGERVEGRFVFFRTNGDDWMVHRM
                     GTAVLPPQRTHPPRLVRPMLATPGELPSGPDDAWAFEMKWDGVRAVTYVDAGSVRVLT
                     RNDREVRATYPELAGLGDVLGDHAVVLDGEIVALDEAGRPSFGVLQQRMHVQRPGRSL
                     LETVPVVYLVFDLLWVDGRSLLDAPYADRRQALEALGLTGPHWQTPPAFPGDGQAASA
                     ASREQGLEGVLAKRVGSTYQPGRRSRDWVKVKHIRTQEVVVGGWLPGQGWRSDTIGAL
                     LLGVPDPDGRLVYAGRVGTGFTQSMLDDLLARLGRLAQKSSPYADALPAADARQARWV
                     APEIVGEVAFSEWTRDGRLRHPRWRGLRTDKAPKDVRRES"
     gene            12576..13808
                     /locus_tag="VLC50_03895"
     CDS             12576..13808
                     /locus_tag="VLC50_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007075308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HSF26641.1"
                     /translation="MPRPQQLSLAQARRVALAAQGFGVARPTRPPDRRALTAAVRRLG
                     LLQIDSVNVLTRAHYLPVFSRLGAYDRDLLDGLAWGPRRSRRLFEYWGHEASLLPVET
                     QPLLRWRMRRARDHAWGWMRRIAAEKPDLVRWVLEEVRRRGPVRAADVEADLAGERPR
                     GEWGWNWSEAKRALEFLFWAGEVTAAKRVNFTRWYDVPERVLPRAVLDAPTPTDDEAA
                     RALVAIAARAHGVATEVCLRDYFRLPVDMARAATAELVEAGELTPVRIDGWRRPAYLH
                     RDARLPRRVDARALLAPFDPLVFERSRVEGIFGFRYRLEIYVPAPARVHGYYVLPFLL
                     GDRLVARVDLKADRVNGGVLRVQAAYAEAGAPPETGPALAAELAEMAEWLGLGGVEVA
                     RPWRGDLAAALVAELRAA"
     gene            complement(13821..14948)
                     /locus_tag="VLC50_03900"
     CDS             complement(13821..14948)
                     /locus_tag="VLC50_03900"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HSF26642.1"
                     /translation="MTTPASDDDPQLGDGTVTLRRLRAGDLDAVVEQSRDPATIRWTR
                     VPVPYARWHAADFLARVAAGWAKGTRRTLAVEAEGRYAGAVELRLDGHGGAEIGFVLS
                     PWARGRGLMSRAVRLLLAWGFDTQGLTVVHWHAHAGNWSSRRVAWACGFRYEGTIRDL
                     LVCRGERRDAWVGSLRRGEPMVPAHRWLAAPVITGRALVLRPWREEDAERVAAACADP
                     VSQWWLPQLPAPYDVCDALGFVRSREEEHAAGRGIFWCVADPGDNRCLAAMSLMIETR
                     SPDAAEVGYWAHPDARGRGVVTEAVRLAARHAAVAEDDGGLGLHRLVLRAAAGNTASR
                     RVAERAGFVEVGRELEGERHRDGSREDFVRYELLTRDVVAG"
     gene            15136..17979
                     /gene="secA"
                     /locus_tag="VLC50_03905"
     CDS             15136..17979
                     /gene="secA"
                     /locus_tag="VLC50_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HSF26643.1"
                     /translation="MSALLDKILRAGEGKTLRKLQRLAGMVNALEEHFVGMTDAELRG
                     MTDEFKTRLAEGETLDDLLPEAFACVREAAKRTLGQRHFDVQMMGGAALHLGNIAEMK
                     TGEGKTLVATAPVYLNALAGRGVHVITVNDYLAAYQSELMGRVYRFLGLEVGCIIAGQ
                     TPTQRREEYAKDITYGTNNEFGFDYLRDNMAWSKDELVQRGHYFAVVDEVDSILIDEA
                     RTPLIISGPADQATQWYTTFAQLVPRLTREADYEVDEKKRTVGILESGVEKVEDYLGI
                     DNLYEAVNTPLVGFLNNALKAKELFKRDKDYVVINGEVLIVDEHTGRILAGRRYNEGM
                     HQAIEAKEGVEIKQENQTLATITLQNFFRLYEKLSGMTGTAMTEAAEFAQIYKLGVVP
                     IPTNKPMIRMDQSDLVYKSEQAKFAAVVDDIAERHEKGQPVLVGTTSVAKSELLSQLL
                     RKRGVPHEVLNAKQHDREAPIVAQAGRQGAVTVATNMAGRGTDIMLGGNAEFMAVAEM
                     RQRGLDPQEDPQAYEAEWPKVLELMQQRVEAEHDAVIELGGLYVLGTERHESRRIDNQ
                     LRGRSGRQGDPGESRFYLSLGDDLMRLFNSGMVETFLTRFNIPEDVPIESKMVSNAIK
                     SAQTQVEAQNFEIRKNVLKYDEVLNRQRTVIYDERRRVLEGMDLEEQIRHMLDDIVEG
                     YVTAETSEGFAEEWDLDRLWTALRTLFPVSLTVDDLVAEVGGERSGLTPEFLIEAIRA
                     DAQQAYDRREEQLGEEVMRELERRVVLSVLDRKWREHLYEMDYLQEGIGLRAMAQRDP
                     LVEYQREGYLLFQAMMDAIKEESVGYLFNLEVEVKAATAPAAGQTAAPAVASDEAAPA
                     QEAAEPAPAAMAAAAGGGRPAIVAKGLSAPTQGRLQYSSPTVDGEAGVAHRADAAADN
                     PYAGTPRNAPCPCGSGRKYKRCHGGPQGAALQA"
     gene            complement(17988..18566)
                     /locus_tag="VLC50_03910"
     CDS             complement(17988..18566)
                     /locus_tag="VLC50_03910"
                     /inference="COORDINATES: protein motif:HMM:NF039460.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rv3235 family protein"
                     /protein_id="HSF26644.1"
                     /translation="MTSTAILRRLPLPDSEPPYDDETSIGQRPRRIAVAATQGTLALA
                     FSLPSGLSAVPALPRMLRLVPAPAAAPAETPGASSDVARLCDPRLWAGRLVQAMLEVL
                     TGDRPVAQLLRWTSAETYADLARRAHTAGRVGAPPLREARALVRSVHVCQPDADVAEV
                     TATVQSRGRTRAVALRLEGCDGRWQCTALQFV"
     gene            complement(18577..>19154)
                     /locus_tag="VLC50_03915"
     CDS             complement(18577..>19154)
                     /locus_tag="VLC50_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018635342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF26645.1"
                     /translation="LNDLLAVVALAAAWALLSWLAMGVAVCALASSPRALGGVLVRAA
                     EVLTPALLRRLVGALVGGAVLVGPVFGAQAATASQDLGAARAAVPMSVAAGPGHAGPG
                     AGAPAPVVFDRPEAVRPVSVVVRPGDALWTIAARALAASGVDPSPAEIAAEWPRWHAA
                     NRQVIGDDPDLLRPGQVLAPPAPPARPTPSR"
BASE COUNT         2593 a         7008 c         6878 g         2675 t
ORIGIN      
        1 gtcaggagcc gccccgggtc tccctcgacg tggcctccaa cgaggtcgac gtgacgagca
       61 tccccgacgt gctgcgcgag aaggtgcgca ccgagccgac ccagcagtgg cgcttcgtcg
      121 agcagcgtta ccgcgacggc cgggtcgaac cgggcctggc cgtgggcgcc cgggtggtcg
      181 tccccgaggc cggcacttac gagctctact acctcttccc gctcacccac gagcacgaga
      241 ccctctccct cgtacggcgg acgctgacct tcgccgggct cgcgttgatc ctgctggtcg
      301 gggcgatcgc ctggctggtg acccggcagg tggtcacgcc ggtgcgcgcc gccgcccgca
      361 tcgccgaacg gctggcggcc ggccacctcg aggaacggat gagggcccac ggcgaggacg
      421 acctggcccg gctggccgcc tcgttcaacc ggatggccac caacctgcag cggcagatcc
      481 gccggctcga ggagctctcc cgggtgcagc gccggttcgt ctccgacgtc tcccacgagc
      541 tgcgcacccc gctgaccacg gtgcggatgg ccgccgacgt catccacgag atgcgcgagg
      601 acttcgaccc ggcggtgcgc cggtcggccg agctgctgca ggcccagctc gaccggttcg
      661 aggcgctgct ggccgacctg ctcgagatca gccggttcga cgcgggcgcg gcggtgctcg
      721 acaccgaacc catcgacctg cgcgacctgg ccacccgggt gatggaggcc aacgagccgt
      781 tggccgcccg gctcggctcg accgtcaccc tggtcgcccc gccgaaacct tgcgtggccg
      841 aggtcgactc gcggcgcatc gagcggatcg tgcgcaacct gctcgtcaac gcgctcgagc
      901 acggcgaggg gcgcccggtc gtggtgcggg tggccgccga cgaggccgcc gtcgcgatcg
      961 ccgtacgcga tcacggggtg gggctgaagc cgggagaggc cgccctggtg ttcaaccggt
     1021 tctggcgggc agacccggcc cgggcccgca ccaccggtgg caccgggctc ggcctgtcga
     1081 tcgcgctgga ggacgcccac ctgcacagcg gatggctgca ggcgtggggc gacccgggtg
     1141 acggctcgca gttccggctg accctgccgc gactcgccgg tgccgacatc gtcgattccc
     1201 cgatcccgct cgaaccggcc gatgcggctc gggggcgagc cgcagtcagc gccggggcgt
     1261 ccacccccgt gccgccgtcc gggacggcgt catgaccacc agacgtcgta cgacgaaggc
     1321 tgtgctggcg ctgctgtcgg cggtcgtgct ggccgccggg tgcgcccaga tcccgacctc
     1381 cgggccggtg cactccggac gcgagctggg cgtcaaccag cccgacagct atgtccgggc
     1441 cctgccggcg ccgcctcggc tgggtgcgac gccggtcgag atcgtgcgcg ggttcctcga
     1501 cgcctcggcc agcttcgagg acgacttcgc ggtggcccgg atgttcctcg agcccgacac
     1561 ccgcgagagc tgggaccccg gcaaggggat caccatctac ggcacgggcg agggcttctc
     1621 gctggccccc agccgaggcg gcggggtcgg gatgagtgcg ccgacggtgg cgacgatcag
     1681 ccccgagggt gtacgccgtc ccgccgacgg cgccgatccg gtcgccgtga cgttcgggct
     1741 ggtccgcgtg caggggcagt ggcggatcag ctcgctgccc gacgggctgc tgctcaccga
     1801 catcgacgtg agccggtcct atcgcgcggt caacatctac ttcctcgacc cgtcccagca
     1861 gatcctcgtg cccgacccgg tcttcgtccc caagtcccca ggcacgtcca ccgcggtggt
     1921 caagcgactg ctgcgtgggc cctcgacctg gctagacccg ggcgtcacat cggcctttcc
     1981 cgtcggcacc gccctgtcgg ggtcggtgcc ggtggagaac ggcgtggccc tggtcgacct
     2041 caacgacgtc gcgctgagcg cctcgcccaa ggcccgtgag gcgctgtcgg cccagctggt
     2101 ctggacgctg agccaggtcg aggacgtgca gtcggtgcgc atcaccgtcg gcgggacgcc
     2161 ctactccgtg cgaggcgccg gcgaggtgca gccgcgcgag tcctggcagg acttcgaccc
     2221 gggcgcgttg cccagcgacg cggtgccgca catcgtgcgt gacaacaagc tgggcatcct
     2281 cgacgacgac accttcaccg cggtgcccgg tccgctcggc tccggcaagc tgcctctggt
     2341 cgacccggcc ctgaccccgc gggccgacct cgtcgccgcg ctgagcgagg accggaccag
     2401 cctgctggtc ggctcggtcc gcagcgagct gaaggctcgg ctgaccggtg gcgacctcac
     2461 ggcgccgtcg tgggacgtcg tgcctaccac ctgggtcgcc gaccggtccg cgccgggcgc
     2521 gttgtggatg gtgccccgct cgggcgagcc ggtgcgggtc aaggtcgacg acatcgcgga
     2581 ccggccgatc accgcggtgc gggtggcccg cgacggcacc cggatcgccc tggtggccgg
     2641 cgccggcgag gccggtcggc tctatgtcgg ggcgatcgcg cgcggcgacg ggcacgacaa
     2701 cccggcccgg gtcgaggggc tgcgcctgct cagcccggcg atcacgagca tcaccgacgt
     2761 cgactgggcc gaggcggacc agctcgcggt gctcggccga gtcggcaagg ccgacccggc
     2821 gatctacctc gtcgacctcg acggtcaggc ggtcaccgcc accccggcga tcccgacggt
     2881 ggtgggtctg gccgcggccc cctggccgct gccgatgatc gcggccacgg gcgacgggca
     2941 gatctatcgg ctggtcgggc gccgctgggt gccggtgggc gagggcatcg caccgttcta
     3001 ccccggctga gccgccaccg tccacagccg tcggtgcggg cccgctcgtc cacaggaccg
     3061 gcctgcccag cctggtgccg cggcacggtc gggtggggtg caggcatgga tgcccttgac
     3121 ccctccgcga ccgcggccgg gacggtgcgt cgggtgccgg cccggctgct ggccaccgct
     3181 ctggacctgg tgctgcccca gcactgcctg ggctgccagg cgcccggggc gccggtctgc
     3241 cacgactgcg ctcgaccgtt gcgcgccagc ccccgccgcg tggcgcccac cccgaccccg
     3301 gtcggcttcc cgccgacctg ggcggtggcc gactacgacg ggctcgtgcg caaggcggtg
     3361 gtggcgcaca aggaggaggg gcgccgagac ctggccccgc tgctgtcggc cgccctggct
     3421 gcgtcgctgc gggccgccgt cgacgacgcc ttcggtgaca caaacccggc ggggggcccg
     3481 gagatcggcg cgcgacatcg acccggccgg cccatcccgg tgctcgtggt gccgatcccg
     3541 tcacgcccct cgtcgcggcg ccagcggggt gacgacccca ccgggcgact ggcacggcgc
     3601 gcccttgccg cgctggccgg ctcgcggccc ggtctgcacc gggtcgggct gctgcgccac
     3661 gcccggggtg tcgccgacca ggccgggcta gacgctgcgg cccgggcggc caacctggcc
     3721 ggcgccctgg ccgcacggcc gctgcctcgg ggggtcggtc gaggcccgct cgtggtcatc
     3781 gtcgacgacg tgctcaccag cggggccacc ctgaccgagg cggcgcgggc gctgcacgcc
     3841 ggcggggcgg gcctggtggt ggctgcggtc gtcgcagcga cggtccgtcg cctggtgccg
     3901 gggggccgcg acgactagcg tttcgtcatg gcacccgctc gggtccgtgg ttgcgccgca
     3961 ggcgagccgt cccacgacgg gaatggctca ggtgcctgca gcaagccgaa gccagccgca
     4021 ggcaaaacgg cccacgtaag gcgacttctc gtcgaccgat cacggtgcgg cttaggaata
     4081 agccctgccc cgccggtgac cccgatggtc gccgtaccgg gagaaggggg gtagtggcgt
     4141 agagccgcga gaccctcagc aggcggatgt ggggacgaag accaggtcgg ccgagcgggt
     4201 gccgccctac ctcgagcgtc cagggaggtc tctgccgtgg agatcgtcgt caagggccgt
     4261 cgtaccgagg tgtcgcagcg gttccgccgg cacgtcatcg aaaagctcgc caaggtcgag
     4321 cggctcgcac cccgggtgat gcgcgtcgac gtcgagctct ccagcgagcg caacccacgg
     4381 cgggccgagc agagccaacg cgttgagctg acctgccggt cgaagggccc ggtgatccgg
     4441 gcggaggcgg ccgccgacga cctctacgcc gcccttgacc tcgccttcgc ccggctcgag
     4501 caacggctgc gtcgtgccgc cgaccgccgc cgggtccacc acggcagccg caccccggtc
     4561 tcggtggccg ccgcgacctc gacgctggcc tccgccacct cggccggaca gggcgcggcc
     4621 gggccagccg cggaggcggc ggcgccaccg ttgggggcgg ccgacgagca tctcgcgccg
     4681 ctggtgggcg tcaacggcga gggcccgatg gtgatccggg aaaaggtcca tgccgcggtg
     4741 ccgatgagcc tcgagcaggc gctctacgag atggagctgg tcgggcacga cttcttcctg
     4801 tacgtcgacg cgcgcagcgc tgccccgagc gtggtctacc ggcgccgggg ctatgactac
     4861 ggggtcatcc gtttggaggt ggccgagcgc gtcgaggact gagcgcaacg ggccactccc
     4921 ggatagtctg cacgccacgt ggggcatcgt gtcgacgcct cggtgcggcc acggcactgt
     4981 ccgtgtgtgg gtgggagagg gcgcgcgatg gatgtggaga ccacgggtgg ccccatcccg
     5041 gccgagccga tccgggtgct gatcgtcgac gaccacgcgc tgttccgccg cgggctcgag
     5101 atggtgctcg cccaggagac cgacatcgag gtgatcggcg aggccgccga cgggtccgag
     5161 gccctggtga tgtcccgtga gctggccccc gacatcgtgc tcatggatgt gcgcatgccg
     5221 cgccgcagcg gcatcgaggc ctgtacggcg atcaaggacg ccgtgcccag cgccaagatc
     5281 gtgatgctga cgatctccga cgaggaggcc gacctctacg aggcgatcaa ggcaggggcc
     5341 agtggctacc tgctcaagga gatctccatc gacgaggtcg ccgacgcgat ccgcgccgtg
     5401 cacagcggcc agtcgctgat cagccccgca atggcctcca agctgctcac cgagttcgcc
     5461 acaatgatca aacgctccga cgagcgggcc caggtgccgg cgccgcggct gaccgaccgt
     5521 gagctcgagg tgctcaagct cgtggcccgc ggtctcaaca accgcgacat cgccaaggag
     5581 ctgttcatct ccgagaacac cgtcaagaac cacgtgcgca acatcctcga gaagctccag
     5641 ctgcactcgc ggatggaggc ggtcgtctac gccgtgcggg agaagcttct cgagatcaca
     5701 tgagcgggct cgtcgccttc tcctcctcgg cgcccgcctc ctgtccctcg ccctgttgcg
     5761 gcacctgacg accctcctgc cccttctgct cctgccccgg gcgctccggc tcgtcggtct
     5821 gctgctgcgt cttctcggcg cgctccttcg gttgcgcccg tccgtcctgg tctgcctcgt
     5881 cgccggccgg cacgacctcg ccctcccgga tctcaccgcg ccaggcaccg gtcgcatcac
     5941 cggacatcat cacgaagcgc cggtagtgct tgaggtcgag gcgtgctcgt cggcccacga
     6001 cgcgccagcg gttgcccatc cactcgacga agcccttcgg ctggtactcc agcaccagca
     6061 ggagcagggt caggtcgtcg gcgagcgggt ggaaggtgat gacgccgtcg accgtgccct
     6121 tgtcgccctc ggagctccac gcgatccggc gctcgggcac ctgctcctgg atggtcgtcg
     6181 tccactcccg ggtggacaga ccgatcttgg ccacccagcg actggtggcg tcgtccttct
     6241 gttccactga ctgtggaccc ttggcgtagg acggcagctc ctggaactgc gtccactggt
     6301 tgtaggcgac gtcgaccggg actccgacaa agatgtcctc gacgatgctg gtgggcagct
     6361 tcttcttccc gccgccgtcc ttgtcgccgc ccccgaagat cccgccgacc ttcttcttca
     6421 ccccgctggc cacgcccgtc agtgcggcct tcggcgcgct ctcgccctcg gccatcttct
     6481 ctgccgtctt gcccgcggca gcggccttga agccgccggt gtcggcggtc ttctccaacg
     6541 aatgggtgag cccgctcagc ttgtcaccca ggcccttgac ggtgcccgac cccttggcgg
     6601 tcacgtagtc acgcacagcg tcggtgagct tggcgaacgt gccgccaccc tcctgctccg
     6661 ggctctgctg ctctgacgtc ttctcctggt cggtcgtcgc gctcatcctt tgactccctc
     6721 tgtgctgctg gacttctgcg gtgtccgctg cgcgggtgtg cgcccggagg ggcgctggct
     6781 ctggcgggac gtgtcctcgc cgcggccctc gggctcctgc tcgtcctgct cgccctgctg
     6841 gccccgctgg tcttgctcgc cctgctcgcc tgctggttct cgttcctctt gtggcccttc
     6901 gtcctgctgc ttgccgccct tgccggcggc gcctgcgccc tgctcaccca ccttgctcaa
     6961 cgcctcggtg cgctgctgca gcgaggtggt cagcgaggcc gcgcgcgcct ccaccgtggc
     7021 gcgcatggcc tgacgcccgg cctgggccag cgatccacgc agctggctga ccagcgcggc
     7081 ggtctcggga tgccgtgcga cggtgagcac gccggtgcgc accaaattct tgggtgtgta
     7141 cttgttgccg cctgcgagcc acaacgccag cgtgagcccg gccctgccct tcttggtgcg
     7201 tcccaggaca tatccgaccg cggtggcggc tcccatcttg atcatgttgg ggttaggcgt
     7261 gtcgttcacc gatgcctcct tctgcgggcc acgtggcccg tcaccgtgcc caggtgctga
     7321 ggaccagccg tccccaacgt gtcgttccgc tcaccacccc catcccgacg agcgctgctg
     7381 atcggccgct gtctcggcga gcacgtcagc aaggaacgcc agcggtggca gcggaccgac
     7441 gtagtccagc cgcgcgtttg cgggcagcgc ctctcgcatc cgggcgacgg cggcgtccat
     7501 gtcgggcagg tggcccctga ccagcaggaa ggcggcggcc tgccgctcgt ggtcctgtgc
     7561 cagcacctgg acccggtcgg cgatctcgac cacaggagcg accaacgccc agaacatgct
     7621 ggcgtgacgg tcggtcagcc gggtcaccac ctgctccccg agcgtcagca gcgccgagcc
     7681 accaggggcc tcagcgcggc tggcctggct gagcctgcgc agctgctcgt cctcggccac
     7741 gacggcgcga agcgacgtct gctcgtcgaa gtgcactcgc agccgcacct cgatcaggtc
     7801 ggccaccgac tcgacctgtc ggcgcagcac ctcgcccgcg ggccgcagca gctcgcgccg
     7861 gaccgcctcg tcgtcgggtg cgacggtcgc gaaggagacc ggcagggccg ggatggtctc
     7921 ggcaagggcg ctgagcagct cgaggtgcgg ggccgcgtcg cgctcgtcga tctcgccggc
     7981 gacgtcggag accaccgcgg ccacgacgtc atcggcgacc acccgggcgc cgtgctgcgc
     8041 gaggccggcc agctggccgt cctcggcgcg gatgatggcg tgcacgagca gggtcatccc
     8101 tgcccgcccc ggtcgggtgg ctgacgggag gccggcgccg accgcgaggc accggtctgc
     8161 gccggcaccg ggctcggttg ttcggcgctc tcgcgtggct cgttctcgcc ttcgccctgg
     8221 tcgcccttga atgacgagat gacgtcttga gcggtctcct tggcaccccg cagcgcgccc
     8281 ttggtcttgt gctcggcgcc cccttcggtg acctcctcca tcagaccggg cagaccagcc
     8341 acttgcttct gcggctgcag gtcgagccgg ttgaccgcct cggcgaagcg gaggtaggtg
     8401 tccacgcttg ccacgacgat gcgggcgtcg atggtgagca gctcgatccc gaggaccgcg
     8461 acgcggacgt aggcgtcgat gacgatgccc ttgtccaaga tgacgtcgag gacgtcggca
     8521 aggctgctcg gtcgaggggt gcgatagaca ctcccgtgcg tgaccgtcac gacggtgtcc
     8581 tccttctcgg caggtgatcg acggtgtcgt caggcaagag cgcggtgagg tcgaccggca
     8641 ggtagaggtc ctcgggcgtg acgccaaacg tgtcggccag ctcgtcgaag gtccgctcga
     8701 gctccatgag agcccggccg agccgctcga cctcctcact gctgaggccg ccagcgtcga
     8761 cacgtcgcag cgcctgccgc tcgatcacct ggcggacgac ctcgagcagc gcgaccacga
     8821 gccggcccag gctgcgcccg acgtcttcgc cggagccatc gatgcgctcg cttgaggcgc
     8881 gggaggcggt catgaggccg cccgccggtc gcgggtgtcg gaggcctcga tgccggtgat
     8941 ccctgtgagc agcagccgca ggtccagccg caccaggtcg atgccggcca ggcagatggt
     9001 gacgtctccg ctgacgaacg cgcctcgttg caccacccgg tcgagcaggt cgaccaggct
     9061 tgttccgctg ctggtggtgc cgcccaggat cccgggcctt tcagctgtgg gcatcggtta
     9121 cctcctgcgc gagcgtggcg aaggagtagg gcggccacgg gccggtcagc tcgaggtgca
     9181 acccgtgacc gatcagctcg cgggccagcc ggtcggtgtc ggcccgaaag cgtgcctcgg
     9241 tagcggtggc gacgagatag gcggcgtcga gcagcagcgc tgacccggcg ctgccgccgc
     9301 gatgaatgac atcgacggtg tagtcgagct ccgcggcgac cttgtcggcc gccagcgcgg
     9361 cgatctcgtt gtcggcctgg tgggcgcgca gggcctcccg gcgacgcgca aggtattccg
     9421 tgcccgccct ggccgagggg ccggtgccat ctgggcgccc accaggctcg acgccgccgg
     9481 cgctggtcgc tggagcgcgc accagccgca ggccccactc ggtggcgccc tcgacgtggc
     9541 gcagccgctc tcgtgccacg tcggcatgcg cccggagcag ctgctgggcc ccagcctcgt
     9601 cctccaccac cgtggcgaag cgcagcggca gcaccgccgc gtgctcaaag accgcccgga
     9661 ccaccgcgtc gtgcccgcgc accagacgcg ccaggtcgcc ttcctcggag aggtcatcct
     9721 cgtcgacgtc gaggccggcg aggcgctctc ggctcaccac cagcgcgagg tcgccgtccg
     9781 gcagaagggt ggcgcgaccc tgcccggtga gcgagcgggc cggcggcatg gccgtgccgg
     9841 cccaggtgat cccgtagcag tagagcccct cgtccggtcg agaggagccg tcgacggtgc
     9901 gggcggccgg gaccctcacg gccggggggc cgtctcgggt gcgggccggc gtgctgggct
     9961 cggcgggggc caacgtgtcg agcgcggcgg tgacgagggc gtcagcgagc aggctcgaca
    10021 gcctttgcac cgcggtgtcg tgggcgatcc gcatcgcctc ctgggcgaca ccggcggccc
    10081 gatccgagac cagtcgggcc agctcgttgc ggtcagcggt cggcatggcc ggtctccgcc
    10141 gttgcgtcgg tctctgggcg gtcgggaccc tcgggagcgg cctcgatgcc gtgctgctgc
    10201 aagagcgctt cgagctgggc gacccggtcg cgcagctctc ggcgctcgtc acgtgccgcc
    10261 acggagctga agaacgggtc ggtggtccac cagtccatgc cgagctcgcg cgcggtgtcc
    10321 gcggaggaga cgaagagccg gagccgaagc gtcagcagct cgagatcgag cacgttgacc
    10381 acgacatcgc cggcgatgac gatccccttg tcgagcaccc gttcgagcac gtcggccaac
    10441 gagctgggct ggctgcgcag ggcgccctgc accggcggag aggactgcac cgtcacgacc
    10501 ggtccaccgc ccctctcgtg tagcgacgga ggcgttcgaa ccccgtcaag gcaccctgcg
    10561 cgtcgaggtc gacccggtag gtggccatca cgctcgtcgt gtctgggatg cgctcgagct
    10621 caaggacctc cacgaggacc gaccagccgt cgccctcctt gcgcacgccc gacacgtcct
    10681 ccggcgcgtg cccggtcacc tcgtcaaagg cggccagcgc ggagcgcacg atcgcaccca
    10741 cgccgtccga tccgggcgtg ggcgatgggt cttgaccttg gttcacgaca tccccctcgt
    10801 gctggcggtc ttgagggcct tggccgaggt gttcaaacgg tcagggaaac aacacgccac
    10861 agccgagagg acggggatgt gctgcgtgga tgggtggatc ggccagcgtc ttgaccgtcg
    10921 gcggagcccc tggagctcat cgcctcggcc ttcatcctcg ccatcgggtg gcgggttggc
    10981 aaggcgccgg gtcgaatgtc acgactcgcg tcggacgtcc ttgggggcct tgtcggtgcg
    11041 aagcccgcgc cagcgcggat gccgtagccg tccgtcacgg gtccactccg agaacgccac
    11101 ctcaccgacg atctcgggag cgacccagcg ggcctggcgg gcgtcggcgg ccggcagggc
    11161 atcggcgtac ggcgagctct tctgcgccag ccggccgagc cgggccagca ggtcgtcgag
    11221 catcgactgg gtgaagccgg tgcccacccg ccccgcgtag accagccgtc cgtcggggtc
    11281 ggggacaccg aggagcagcg ccccgatggt gtccgagcgc cagccctgcc cgggcaacca
    11341 gccgcccacg acgacctcct gggtgcggat gtgcttgacc ttgacccagt cccgcgaccg
    11401 ccggcctggc tggtaggtcg agccgacccg tttggccagc accccttcca gcccctgctc
    11461 gcgcgaggcg gccgacgcgg cctggccgtc accggggaat gccggcgggg tctgccagtg
    11521 cggcccggtt agccccagcg cctcgagggc ctggcggcgg tcggcgtacg gcgcgtcgag
    11581 caaggagcgc ccgtcgaccc acagcaggtc gaacacgagg tagaccaccg gcaccgtctc
    11641 gagcagggag cgccccgggc gctgcacgtg catccgctgc tgcagcacgc cgaacgacgg
    11701 gcggcccgcc tcgtcgagcg cgacgatctc gccgtcgagg accacggcat ggtccccgag
    11761 cacgtcgccg agcccggcca gctccgggta ggtggcacgc acctctcggt cgttgcgcgt
    11821 gagcacccgc accgatccgg cgtcgacgta ggtcaccgcg cgcacgccgt cccacttcat
    11881 ctcgaacgcc catgcgtcgt ccggtccgct gggcagctcg cccggggtag cgagcatcgg
    11941 gcggaccagc cgcggcgggt gggtgcgctg cggcggcagc accgcggtgc ccatgcggtg
    12001 caccatccag tcatcgccgt tggtacggaa gaagacaaac ctgccctcca cccgctcgcc
    12061 gtgcagcacg accttgacct cgtcgtcggt ccacttctcg cactcgtagg tgccccggtc
    12121 ccagatgatc acctggccgc cgccgtactc gcccttgggg atccatccgg cgaagccggc
    12181 gtactcgagc gggtggtcct cggtgtgcac cgcgaggtgg ttgcgcttgg ggtcgtcggg
    12241 cagcccgcgc ggcaccgccc aggacaccag caccccgtcg cgttcgaggc gcacgtccca
    12301 gtgcagccgg cgggcgtggt gctcctggat gacgaacgtg tcgtcgtcac cgcgcggcag
    12361 cggcccgacg ccggggaccg gctcgggcgt gcggcccggg tcgcgcttgc tgcggtaggt
    12421 ggccagcgcg tcgtcgtcag ggggagcggc gggcggcttg cgggggctca ccctcccgat
    12481 gatgcgcgtc gcggcgcctc cctctcgccg tgatcaagca agtcgtgctc ggcgccgagc
    12541 gcagcggtgt cggcgggtgc tggcacgatg ccctcgtgcc ccggccgcag cagctctcgc
    12601 tcgcccaggc gcgccgggtc gcgctggccg cgcagggctt cggcgtcgcc cggccgaccc
    12661 gtccgcccga ccggcgtgcg ctgacggccg ccgtacgccg gctcggcctg ctgcagatcg
    12721 actcggtcaa cgtgctcacc cgggcccact acctgccggt gttctcccgg ctcggcgcct
    12781 atgaccgcga tctgctcgac gggctggcct gggggccgcg gcggtcccga cggctgttcg
    12841 agtactgggg tcatgaggcg tcgctgctgc cggtcgagac ccagccgctg ctgcgctggc
    12901 ggatgcgccg ggcccgcgac cacgcatggg gctggatgcg gcggatcgct gcggaaaagc
    12961 ccgacctggt gcgctgggtg cttgaggagg tgcgccgccg cgggccggtg cgggccgccg
    13021 acgtcgaggc cgacctggcc ggcgagcgcc cgcgcgggga gtggggctgg aactggagcg
    13081 aggccaagcg ggcgctcgag ttcttgttct gggccggcga ggtgaccgcg gccaagcgcg
    13141 tcaacttcac ccggtggtac gacgtgcccg agcgggtgct gccccgggcg gtgctcgacg
    13201 cgcccacgcc gaccgacgac gaggcggccc gagcgctggt ggcgatcgcg gcccgcgccc
    13261 acggcgtggc cacagaggtc tgcctgcgcg actacttccg gctgccggtc gacatggcgc
    13321 gggccgcgac ggccgagctg gtcgaggccg gcgagctgac cccggtgcgc atcgacggct
    13381 ggcgccgacc ggcctacctg caccgcgacg cccggctgcc ccgccgggtc gacgcccgcg
    13441 cgctgctggc gccgttcgac ccgctggtgt tcgagcgcag ccgggtcgag ggcatcttcg
    13501 ggttccgcta ccggctggag atctacgtgc cggccccggc gcgggtgcac ggctactacg
    13561 tgctgccgtt cctgctcggt gaccggctgg tggcccgcgt cgacctcaag gccgaccggg
    13621 tcaacggggg agtactgcgg gtgcaggccg cctacgccga ggccggcgcg ccgccggaga
    13681 ccggcccggc gctggccgcc gagctggccg agatggccga gtggctcggg ctgggcgggg
    13741 tcgaggtggc ccggccctgg cgcggtgacc tcgcggcggc gctggtcgcc gagctgcgcg
    13801 cggcctgacg cggcacttgc tcagccggcg accacgtcac gggtgagcag ctcgtagcgc
    13861 acgaagtcct cccgcgagcc gtcgcggtgc cgctcgccct cgagctcgcg gcccacctcg
    13921 acgaacccgg cccgctcggc cacccggcgc gaggccgtgt tgcctgcggc cgctcgcagc
    13981 accagccggt gcaggcccag cccgccgtcg tcctcggcca ccgcggcgtg gcgcgccgcc
    14041 agccgcaccg cctcggtgac gaccccgcgg ccccgggcgt cgggatgggc ccagtagccg
    14101 acctcggccg cgtcgggact gcgcgtctcg atcatcaggc tcatcgccgc caggcaccgg
    14161 ttgtcgcccg ggtcggcgac gcaccagaag atcccgcgcc cggcggcgtg ctcctcctcc
    14221 cgcgagcgca cgaagccgag cgcgtcgcag acgtcgtacg gcgcgggcag ctgcggcagc
    14281 caccactggg agaccgggtc ggcgcaggcc gccgccaccc gctcggcatc ctcctcgcgc
    14341 cacggccgca gcacgagcgc ccggccggtg atcaccgggg cagccagcca gcggtgtgcc
    14401 ggcaccatcg gttcgccgcg ccgcagcgag ccgacccacg cgtcgcgccg ctcgccgcgg
    14461 cagaccagca ggtcgcggat ggtgccctca taccggaagc cgcaggccca cgccacccgc
    14521 cgggaggacc agttgccggc gtgcgcgtgc cagtgcacga ccgtcaggcc ctgggtgtcg
    14581 aagccccagg ccagcaagag tcgcaccgcc cgggacatca ggccgcgccc ccgggcccac
    14641 ggggagagca cgaagccgat ctcggcgccg ccgtgcccgt ccaggcgcag ctcgaccgcg
    14701 ccggcgtagc ggccctcggc ctcgaccgcc agggtgcgcc tcgtgccctt cgcccacccc
    14761 gcggcgaccc gggcgaggaa gtcggccgcg tgccagcggg cgtagggcac cgggacgcgc
    14821 gtccaccgga tggtggcggg gtcacgggac tgctccacga ccgcgtcgag gtcaccggcc
    14881 cggaggcggc gcagcgtcac cgtcccgtcg ccgagctgtg ggtcgtcgtc gctcgccggg
    14941 gtcgtcacgt tggcatccaa ccgggcactc cacgggccgt cgagccgtta accgggaggt
    15001 caccgcctac gatggccagt tggtgggccc cgtctgcgcg gtggccccgc ccgcggggcc
    15061 gccggcccgg cgccagcttc gcaccagact gtcgtcccgc gaccgtccta gcaccgatca
    15121 cgaggagtca tccgcgtgtc agccctgctc gacaagatcc ttcgtgccgg tgagggaaag
    15181 accctccgca agctccagcg tctcgcgggg atggtcaacg ccctggagga gcacttcgtc
    15241 ggcatgaccg acgccgagct gcgcgggatg accgacgagt tcaagactcg cctggccgag
    15301 ggcgagacgc tcgacgacct gctgcccgag gcattcgcct gcgtgcgcga ggctgccaag
    15361 cgcacgctcg gtcagcgcca cttcgacgta cagatgatgg gtggggcggc gctgcacctg
    15421 ggcaacatcg ccgagatgaa gaccggtgag ggcaagacgc tggtggccac cgcccccgtc
    15481 tacctcaacg cgctggccgg ccggggcgtg cacgtcatca ccgtcaacga ctacctggcc
    15541 gcctaccagt ccgagctgat gggccgggtc taccggttcc tcggcctgga ggtcggctgc
    15601 atcatcgcgg gacagacccc gactcagcgg cgcgaggaat acgccaagga catcacctac
    15661 ggcaccaaca acgagttcgg cttcgactac ctgcgcgaca acatggcctg gtccaaggac
    15721 gagctggtcc agcgcgggca ctacttcgcc gtcgtcgacg aggtcgactc gatcctcatc
    15781 gacgaggccc gcaccccgtt gatcatcagc ggtccggccg accaggcgac ccagtggtac
    15841 accacgttcg cccagctggt gccgcggctg acccgcgagg ctgactacga ggtcgacgag
    15901 aagaagcgca ccgtcggcat cctggagtcc ggcgtcgaga aggtcgagga ctacctcggc
    15961 atcgacaacc tctacgaggc ggtcaacacg ccgctggtgg gcttcctcaa caacgcactg
    16021 aaggccaagg agctgttcaa gcgcgacaag gactacgtcg tgatcaacgg cgaggtgctc
    16081 atcgtcgacg agcacaccgg ccgcatcctg gccgggcgcc gctacaacga gggcatgcac
    16141 caggcgatcg aggccaagga gggggtggag atcaagcagg agaaccagac gctggccacc
    16201 atcaccctgc agaacttctt ccgcctctac gagaagctct ccggcatgac cggtacggcg
    16261 atgaccgagg ccgccgagtt cgcccagatc tacaagctgg gcgtggtgcc gatcccgacc
    16321 aacaagccga tgatccgcat ggaccagtca gacctggtct acaagtcgga gcaggccaag
    16381 ttcgccgccg tcgtcgacga catcgccgag cgccacgaga agggccagcc ggtcctggtc
    16441 ggcacgacga gcgtggccaa gtccgagctg ctctcccagc tgctgcgcaa gcgcggggtg
    16501 ccccacgagg tgctcaacgc caagcagcac gaccgtgagg cgccgatcgt ggcccaggcc
    16561 ggccgtcagg gcgcggtcac cgtggccacc aacatggccg gtcgcggcac cgacatcatg
    16621 ctcgggggca acgccgagtt catggccgtg gccgagatgc gccagcgcgg gctcgacccg
    16681 caggaggacc cgcaggccta cgaggccgag tggcccaagg tgctcgagct catgcagcag
    16741 cgggtcgagg ccgagcacga cgccgtcatc gagctcggcg ggctctacgt gctgggcacc
    16801 gagcgtcacg agtcgcgccg catcgacaac cagctgcgcg ggcgctccgg ccggcagggc
    16861 gacccgggtg agtcgcgctt ctacctctcg ctgggcgacg acctgatgcg tctgttcaac
    16921 tcgggcatgg tcgagacgtt cctcacccgg ttcaacatcc ccgaggacgt gccgatcgag
    16981 tccaagatgg tgtccaacgc catcaagtcg gcccagaccc aggtcgaggc gcagaacttc
    17041 gagatccgca agaacgtcct caagtacgac gaggtgctca accgccagcg caccgtcatc
    17101 tacgacgagc gccgccgggt cctcgagggc atggacctcg aggagcagat ccggcacatg
    17161 ctcgacgaca tcgtcgaggg ctacgtcacc gcggagacca gcgagggctt cgccgaggag
    17221 tgggacctcg accggctgtg gaccgcgctg cgcacgctct tcccggtctc cctcaccgtc
    17281 gacgatctcg tcgccgaggt gggcggcgag cggtcggggc tgacgccgga gttcctcatc
    17341 gaggcgatcc gcgccgacgc ccagcaggcc tacgaccggc gtgaggagca gctcggcgag
    17401 gaggtcatgc gcgagctgga gcggcgggtg gtgctgtcgg tgctcgaccg caagtggcgc
    17461 gagcacctct acgagatgga ctacctgcag gagggcatcg gcctgcgggc gatggcccag
    17521 cgcgacccgc tcgtcgagta ccagcgcgag ggttacctgc tcttccaggc gatgatggat
    17581 gccatcaagg aggagtcggt cggctacctg ttcaacctcg aggtcgaggt gaaggccgca
    17641 accgcgccgg cggcaggtca gacagccgca ccggcggtcg cctccgacga agccgcaccc
    17701 gcccaggagg cggccgagcc cgcgccggcg gccatggccg ccgcggccgg tggcgggcgg
    17761 cccgcgatcg tggccaaggg cctgagcgca cccacccagg gccggctgca gtactccagc
    17821 ccgaccgtcg acggggaggc cggagtggcc caccgggccg acgcggccgc ggacaacccc
    17881 tacgccggca cgccgcgcaa cgcgccctgc ccgtgcgggt cgggccgcaa gtacaagcgc
    17941 tgccacggcg gacctcaggg cgccgcgctg caggcctagc tgctcggtca gacgaactgc
    18001 agggcggtgc actgccagcg gccgtcacag ccctccagcc gcagcgccac cgcgcgggtg
    18061 cggccccggc tctgcacggt tgcggtgacc tccgcgacgt cagcgtctgg ttggcagacg
    18121 tgcaccgagc gcacgagggc acgtgcctcc cgcagcgggg gcgcccccac ccggccggcg
    18181 gtgtgcgccc ggcgggccag gtcggcatag gtctcggcgg aggtccaccg gagcagctgc
    18241 gccaccgggc gatcacccgt gagcacctcg agcatggcct gcaccagccg ccccgcccac
    18301 agccgcgggt cgcacagccg agcgacatcc gacgacgcgc cgggcgtctc ggccggtgcg
    18361 gcggccgggg cagggacgag ccgcagcatg cgcggcagtg ccgggaccgc ggacagcccg
    18421 ctgggcaggc tgaaggccag ggcgagcgtg ccctgggtgg cggccacggc gatccgccgg
    18481 gggcgttggc cgatcgatgt ctcgtcgtca tagggcggtt cgctgtccgg cagcggcagc
    18541 cggcgcagga tggcggtcga ggtcatgtcg ggctccttat cggctgggcg tgggacgtgc
    18601 gggtggggcc ggcggcgcga gcacctgccc gggccgcagc aggtcggggt catcgccgat
    18661 cacctggcgg ttggcggcgt gccagcgcgg ccactcagcg gcgatctcgg ctgggctcgg
    18721 gtccacgccg ctggcggcca gggcacgggc cgcgatggtc cacagggcat cgcccgggcg
    18781 caccaccacg gagacgggcc gcacggcttc cggccggtcg aagaccaccg gggcgggggc
    18841 gccggccccc ggacctgcgt gtcctgggcc ggcggcgacc gacatcggca ccgccgctcg
    18901 cgccgccccc aagtcctgag aggcagtcgc ggcctgcgcc ccgaacaccg ggccgacgag
    18961 cacggcacca ccgaccagtg ccccgacgag gcggcgcagc agcgccggcg tgagcacctc
    19021 cgccgctcgg accagcaccc cgccgagggc acggggactg ctcgcgagcg cgcagaccgc
    19081 gacgcccatg gccagccagc tgagcagcgc ccaggcggcg gccagcgcga ccacggcgag
    19141 caggtcgttg aggg
//
LOCUS       DATIBB010000102         5501 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_710639, whole genome shotgun sequence.
ACCESSION   DATIBB010000102 DATIBB010000000
VERSION     DATIBB010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 5501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5501
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_710639"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(17..391)
                     /locus_tag="VLC50_03920"
     CDS             complement(17..391)
                     /locus_tag="VLC50_03920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF26646.1"
                     /translation="MDRASSSEWASSGHPLLAGLHTSGPGLTSAAGLGRDRVSLLSDE
                     DTRSALDAVAAQEAQLVAIRAALVSHAEVRALKTQLKPRTTASWLADTARISGPAARE
                     HVKVAAMLGTVDTATRPWRWSS"
     gene            complement(580..1101)
                     /locus_tag="VLC50_03925"
     CDS             complement(580..1101)
                     /locus_tag="VLC50_03925"
                     /inference="COORDINATES: protein motif:HMM:NF019500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HSF26647.1"
                     /translation="MSGAIPIGLTWATVARSTATTVLIGVLYAVLPVASDRRGASVVI
                     LVTGLIVLGVVIVVRIRQLLSDPRPVVRAIEALSLVIPLFLTVFAWSYLSLSSADPSS
                     FTEPLDKVAAIYFTIVVTGTVGFGDITPHTDLARLLVSGQIVLGLTLFTAVVRVVISI
                     ARERSPHPPRNGR"
     gene            complement(1263..2639)
                     /locus_tag="VLC50_03930"
     CDS             complement(1263..2639)
                     /locus_tag="VLC50_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HSF26648.1"
                     /translation="MTTVTSPRRSLNRDRLAALHAREVARFVAAHPRSARLHERALAS
                     MPEGVPMSWMAKWAGPFPVYVADATGSRFTCVDGHEYVDLCLGDTGAMTGHSPAATVD
                     AVRDRLSRGITLMLPTEDAAVVAEDLGQRFGLPQWQFTLSATDANRHVIRYARHVTGR
                     PKVLVADYCYHGSVDETFATLDDSGRVVARRGNIGAPVPPSETTAVVEFNDLDALEQS
                     LATGEIACVLMEPAMTNIGIVLPDPGYHAALRELTRRHGTLLVIDETHTICAGPGGMT
                     AAEGLEPDVVTIGKAIGGGIPSGAFGMSAELASRIRRSVELEDIDVGGIGGTLAGNAL
                     SLAAMRTTLSSVLTPTAFEAMLPLGASWADGVAGALASYDVPWHVTRLGARAEYAFAP
                     TPPRTGREAAEADDFELQTYLHLHALNRGLLLTPFHNMALMAPTTTAADVDRHTAAFT
                     ECVAELFA"
     gene            complement(2651..4006)
                     /gene="gabT"
                     /locus_tag="VLC50_03935"
     CDS             complement(2651..4006)
                     /gene="gabT"
                     /locus_tag="VLC50_03935"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014145184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HSF26649.1"
                     /translation="MRVTTPYGGPALPQERRLVTEIPGPRSKELHARRVAAVATGVSS
                     GLPVYVAAAGGGVIVDVDDNSLIDMGAGIAVVSVGNAAEHVAAAVRAQVVSFTHTCFM
                     VTPYEGYVAVCEALNRLTPGGHEKRSALFNSGAEAVENAVKIARHHTGRNAVVVLDHA
                     YHGRTNLTMAMTAKNMPYKHRFGPFAGEVYRVPMSYPFREPVPISGEEAARRATALVD
                     KQVGADNVACLVVEPIQGEGGFVVPAPGFLAALASWCREHGVVFVADEIQTGFARTGD
                     MFACEHEGVVPDLITTAKGIAGGLPLSAVTGRAEIMDAAHAGGLGGTYGGNPVACAAA
                     LGAIRTIEEADLVGRARRIEELMLPRLHALAQRHPQIGDVRGRGAMLAIEIVRPGTTE
                     PDAALTAALNAHCHRQGVVTLTCGTFANVFRFLPPLVIPEPLLLEALDVLDDAFATLA
                     G"
     gene            complement(4003..5451)
                     /locus_tag="VLC50_03940"
     CDS             complement(4003..5451)
                     /locus_tag="VLC50_03940"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013731712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HSF26650.1"
                     /translation="MSEHKTVLRNFVNGAYVDTRDQQSTDLVDPSTGEVFGSAPLSGP
                     ADVDAAYAAAAAAFETWGETTPSERQRALLHLADAVEAHADELVALESQNTGKPIALT
                     ASEEIPPMVDQIRFFAGAARVLEGRSAGEYMAGHTSFVRREPVGVVGQVTPWNYPMMM
                     AVWKLAPAIAAGNTVVLKPSDTTPVTTLRLAELAAEFLPPGVLNVVCGDRDTGRALVE
                     HPTPAMVAITGSVRAGMEVAGTAARDVKRVHLELGGKAPVIVFDDADLDAAAEAIAIA
                     GYFNAGQDCTAATRVMASPRVYDDFVGAITEQAKGIVTTYEHGADYEDALLPPVNNAN
                     QLTRVSSFIDRLPDHAQVSAGGTRLGERGYHYAATVVSDLHQDDEIIQNEVFGPVITV
                     QRFDDEAEALRWANGVQYGLASSVWTRDHGRAMRMAKALDFGCVWINTHIPLVAEMPH
                     GGFKHSGYGKDLSMYGLEDYTRVKHVMTNIEV"
BASE COUNT          820 a         1896 c         1979 g          806 t
ORIGIN      
        1 aggcgagcct gccgggtcaa gaactccacc gccacggccg agtggccgtg tcgacggtgc
       61 cgagcatggc ggcgaccttg acgtgttcgc gggccgcggg accgctgatc cgggcggtgt
      121 ccgccaacca cgacgcggtc gttcggggct tgagctgggt tttcagtgca cgcacctcgg
      181 catggctcac aagtgcggcg cggatcgcga cgagctgcgc ttcctgcgcc gccaccgcgt
      241 ccagtgccga tcgggtgtcc tcatccgaga gcagcgagac ccggtcgcgg cccagcccgg
      301 cggcacttgt caaaccagga ccggaggtgt gcagcccggc tagcagtgga tgcccactgc
      361 tcgcccactc gctgctgctc gccctgtcca tgaccccacg ctagatgcca ccaccgacag
      421 tccccgcgaa cccggaaccc gttgtggaca aacgtgttcc ggcccgcgcc tgtggacggg
      481 ccgccccgcc aattgcgcat gatcagcgca ggaggcggcg gcgcagagcc gccattcgcg
      541 catgatcagt gcaggagagg gcggcggggg aggggacggt caccggccat tccggggcgg
      601 gtgcggagag cgttcccggg cgatgctgat gacgactcgc acgacggcgg tgaacagagt
      661 cagcccaagc acgatctgcc cgctcaccaa cagccgggcc aggtcggtat gcggggtgat
      721 gtcgccgaac ccgacggtgc cggtgacgac gatcgtgaag tagatcgccg cgaccttgtc
      781 caacggttcg gtgaagctcg acggatcggc gctcgacaac gataaatagg accacgcgaa
      841 cactgtcagg aacagcggga tcaccagcga gagcgcctcg atggcacgca cgaccggtcg
      901 cgggtcgctc agcagctgac gaatccgcac gacgatgacg acaccgagca cgatgaggcc
      961 cgtcacgagg atgacgacgc tcgcgccacg acggtcggac gcgaccggca gcacggcgta
     1021 gagcacgccg atgagcacgg tggttgcggt ggagcgcgcc accgtcgccc aggtcagtcc
     1081 gatcggaatg gcaccgctca ccgcttcatg taaccctgcg cgtcccgccg ctgtgcacgg
     1141 attgcttgat cacggacgcg aggcgggcac ggatcgcttg atcacggacg cgaggcgggc
     1201 acggattgct tgatcacgaa cgtgaggcga gcagggattg cttgatcgcg acgagggtgg
     1261 ggtcaggcga acagctcggc cacgcactcg gtgaacgccg cggtgtgccg gtcgacgtcg
     1321 gccgcggtcg tcgtcggcgc catcagcgcc atgttgtgga acggcgtgag cagcagcccg
     1381 cggttgagcg cgtgcaggtg cagataggtc tgcagctcga agtcgtcggc ctcggcggcc
     1441 tcgcgcccgg tgcgcggcgg cgtcggcgcg aaggcatact cagcgcgggc accgagtcgg
     1501 gtcacgtgcc acggcacgtc gtacgacgca agtgcaccgg ccacgccgtc cgcccaggac
     1561 gccccgagcg gcagcatcgc ctcgaacgcc gtcggggtga gcaccgacga cagggtggtc
     1621 cgcatcgcgg ccagcgacaa cgcgttgccg gccagggtgc caccgatccc gccgacgtcg
     1681 atgtcctcca gctcgaccga gcgccggatc cgggaggcca gctcggcgct catgccgaac
     1741 gcgcccgacg ggatgccgcc gccgatcgcc ttgccgatgg tcacgacgtc gggctcgagc
     1801 ccctcggcag cggtcatccc gccggggccg gcacagatcg tgtgcgtctc gtcgatgacc
     1861 agcagggtgc cgtgccggcg ggtcagctcg cgcaaggcgg cgtgatagcc cgggtcgggc
     1921 agcacgatgc cgatgttggt catcgccggt tccatcagca cgcaggcgat ctcgccggtc
     1981 gccagcgact gctcgagcgc gtcgaggtcg ttgaactcca ccaccgcggt cgtctccgag
     2041 ggcggcacgg gcgcgccgat gttgccgcgg cgggcaacta cacgccccga gtcgtcaagc
     2101 gtggcaaagg tctcgtcgac gctgccgtgg tagcagtagt cggccaccag caccttgggt
     2161 cgcccggtga cgtggcgggc atagcggatg acgtgccggt tggcgtcggt ggcggagagc
     2221 gtgaactgcc actgcggcag gccgaaccgc tgcccgaggt cttcggcgac caccgcggcg
     2281 tcctcggtcg gcagcatgag cgtgatgccg cgcgagaggc gatcgcgtac ggcgtcgacg
     2341 gtggccgcgg gggagtgccc ggtcatcgcg ccggtgtcgc cgaggcacag gtcgacgtac
     2401 tcgtgcccgt ccacgcaggt gaaccgcgac ccggtagcgt cggcgacgta gaccgggaac
     2461 ggcccggccc acttggccat ccagctcatc ggtacgcctt ccggcatcga cgcgagcgcc
     2521 ctctcgtgca gccgcgccga ccggggatgg gccgcgacaa accgggcgac ctcgcgggcg
     2581 tgcaacgcgg ccagccggtc gcggttgagc gagcggcgcg gggacgtgac cgtggtcatg
     2641 agcgttcctc tcagccggcc agggtcgcga acgcgtcgtc gagcacgtcg agcgcctcga
     2701 gcagcagcgg ctcggggata accagcggcg gcagaaaccg gaacacgttg gcgaacgtgc
     2761 cacaggtcaa ggtgaccaca ccctgtcggt ggcagtgtgc gttgagcgcg gcggtcaggg
     2821 ctgcgtcggg ctcggtggtg cctggccgga cgatctcgat ggccagcatc gccccgcgcc
     2881 cgcgcacgtc gccgatctgc gggtgccgct gggccagcgc gtgcagccgg ggcagcatca
     2941 gctcttcgat ccggcgggct cgcccgacca ggtcggcttc ctcgatggtg cggatcgccc
     3001 ccagcgcggc cgcgcaggcc accgggttgc cgccgtaggt gccgcccagc ccgccggcgt
     3061 gcgcggcgtc catgatctcg gctcggccgg tgactgccga cagcggcagt ccgccggcga
     3121 tccccttcgc cgtggtgatg aggtcgggca ccacgccctc gtgctcgcag gcgaacatgt
     3181 cgccggtgcg cgcaaagccg gtctggatct cgtcggcgac gaagaccacc ccgtgctcac
     3241 ggcaccacga cgccagcgca gcaaggaatc caggagcggg tacgacgaac ccaccctcgc
     3301 cctggatcgg ctcgaccacc aggcacgcga cattgtcggc accaacctgc ttgtcgacga
     3361 gcgccgtggc ccgccgggcc gcctcctcgc cactgatcgg caccggctcg cggaacgggt
     3421 aggacatcgg cacccggtag acctcgccgg cgaacggccc gaagcggtgc ttgtagggca
     3481 tgttcttggc ggtcatcgcc atcgtgaggt tggtgcgccc gtgataggcg tggtcgagca
     3541 ccacgaccgc attgcgcccg gtgtgatggc gggcgatctt cacggcgttc tcgactgcct
     3601 cggccccgga gttgaacagc gcgctgcgtt tctcgtgccc gcccggcgtg agccggttga
     3661 gcgcctcgca caccgcgacg tagccctcgt agggcgtgac catgaagcag gtgtgggtga
     3721 agctgaccac ctgggctcgg accgcggccg ccacgtgctc ggcggcgttg cccacgctga
     3781 ccacggcaat gcccgcgccc atgtcgatga gcgagttgtc gtcgacgtcg acgatcaccc
     3841 cgccgccggc cgcggcgacg tagaccggga gccccgagct caccccggtc gccaccgccg
     3901 ccacccggcg ggcgtgcagc tccttcgaac gtgggcccgg gatctcggtg accaggcggc
     3961 gctcctgggg cagcgcgggg ccgccgtacg gcgtggtcac tctcatacct cgatgttggt
     4021 catgacgtgc ttgacgcggg tgtagtcctc caggccgtac atggacaggt ccttgccgta
     4081 gccggagtgc ttgaagccgc cgtggggcat ctcggcgacg agcgggatgt gcgtgttgat
     4141 ccacacgcag ccgaagtcga gcgccttggc catgcgcatg gcccggccgt ggtcgcgggt
     4201 ccacacgctg gaggccaggc catattgcac gccgttggcc cagcgcagcg cctcggcctc
     4261 gtcatcaaag cgctgcaccg tgatcaccgg accgaacacc tcgttctgga tgatctcgtc
     4321 gtcctggtgc aggtcggaga ccaccgtcgc ggcgtagtgg tagccccgct cgcccagccg
     4381 cgtcccaccg gcggacacct gggcgtggtc gggcagccgg tcgatgaagg aggagacccg
     4441 ggtgagctgg ttggcattgt tgaccggtgg cagcagcgcg tcctcgtagt cggcgccgtg
     4501 ctcataggtc gtcacgatgc ccttggcctg ctcggtgatg gcgccgacga agtcgtcata
     4561 gacccggggc gaggccatca cccgggtggc cgcggtgcag tcctgcccgg cgttgaagta
     4621 gccggcgatg gcgatcgcct cggccgctgc gtcgaggtcg gcgtcgtcaa agacgatcac
     4681 tggcgccttg ccgcccagct cgaggtgcac ccgcttgacg tcgcgcgcgg cagtgccggc
     4741 cacttccatg ccggcgcgca cggacccggt gatcgccacc atcgccgggg tcgggtgctc
     4801 gaccagcgcc cgcccggtgt cgcggtcgcc gcagaccacg ttgagcacgc ccggcggcag
     4861 gaactcggcg gccagctcgg ccagccgcag cgtggtcacc ggggtggtgt ccgacggctt
     4921 gagcaccacg gtgttgccgg ccgcgatcgc cggggcgagc ttccagacag ccatcatcat
     4981 cgggtagttc cacggggtga cctgcccgac gacgccgacc ggctcgcggc ggacgaagga
     5041 ggtgtgcccg gccatgtact ccccggcgga gcggccctcg agcacccgcg ccgcgccggc
     5101 gaagaacctg atctggtcga ccatcggggg gatctcctcc gaggcggtca gcgcgatcgg
     5161 cttgccggtg ttctggctct ccagcgcgac cagctcgtcg gcgtgcgcct ccacggcgtc
     5221 ggccaggtgc agcagggcgc gctgccgctc gctgggcgtg gtctcgcccc aggtctcgaa
     5281 cgccgcggcc gccgcggcat aggcggcgtc gacgtcggcc ggtccggaca gcggtgccga
     5341 gccgaacacc tcgccggtcg acgggtccac caggtcggtg ctctgctggt ctcgggtgtc
     5401 gacgtaggcc ccgttgacga agttgcgcag gaccgtcttg tgctcgctca tctcgacctc
     5461 cggtcatggg atggatgtgt cggcgcgacg ggtcacaggc g
//
LOCUS       DATIBB010000103        11416 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_451212, whole genome shotgun sequence.
ACCESSION   DATIBB010000103 DATIBB010000000
VERSION     DATIBB010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 11416)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11416)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11416
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_451212"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..447
                     /locus_tag="VLC50_03945"
     CDS             <1..447
                     /locus_tag="VLC50_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018382480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSF26651.1"
                     /translation="QERELRGWLYRPVADPDATLAAALERVAADVEDGHDVSVEVVVV
                     GDARLDERLAALLQAAREALVNAAKYAGAAGPISVYGEVEDGQVTVFVRDRGPGFALE
                     AVPADRMGVRESILGRMSRNGGRAVVRSEPGAGTEIELDMPREGAS"
     gene            444..1094
                     /locus_tag="VLC50_03950"
     CDS             444..1094
                     /locus_tag="VLC50_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005481110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HSF26652.1"
                     /translation="MSGERTVRVVIVDDHRMFRTGVRAELGQRVDVVGEADDVDTAVA
                     VISETRPDVVLLDVHLPGGGGREVLRRCVPSLPDTTFLALSVSDAAEDVIGVIRAGAR
                     GYVTKSITGDELAAAIRRVADGDAVFSPRLAGFVLDAFAGAVVPADVDEDLDRLTARE
                     REVLRLIARGYAYKEVGKQLFISVKTVETHVSAVLRKLQLSNRHELTRWATDRRLV"
     gene            complement(1193..2077)
                     /gene="sucD"
                     /locus_tag="VLC50_03955"
     CDS             complement(1193..2077)
                     /gene="sucD"
                     /locus_tag="VLC50_03955"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014742774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HSF26653.1"
                     /translation="MAIFLTEKSRVVVQGMTGSQGRAHTQRMLASGTTIVGGTNPKKA
                     GQTLDFDGATLPVFGTVTDAVKEAGADVSVVFVPPAFTKAAVYEAVDAGIPLVVVITE
                     GVPVHDSAAFYQYAANSGTTRLIGPNCPGLISPGKSNVGIIPATIAKAGRIGLVSKSG
                     TLTYQMMYELADLGFSTAVGIGGDPVIGTTHIDCLQAFEDDPDTDAIVMIGEIGGDAE
                     ERAAAYIAEHVSKPVVGYVAGFTAPEGKTMGHAGAIVSGSSGTAAAKKAALEAVGVRV
                     GKTPSETARLMRELVTAR"
     gene            complement(2092..3303)
                     /gene="sucC"
                     /locus_tag="VLC50_03960"
     CDS             complement(2092..3303)
                     /gene="sucC"
                     /locus_tag="VLC50_03960"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018134363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HSF26654.1"
                     /translation="MDLFEYQARDLFAQHGVPVLDAAVAETPEAARAAAQSLGGGTVV
                     VKAQVKTGGRGKAGGVKLAHSPDEAAAVAEAILGMDIKGHTVHRVMVAPGAKIAQEYY
                     FSILLDRANRTYLAMASREGGMEIEVLAVEKPEALARVPVDAIAGIDEAKAREIVAAG
                     GFPAEVADQVVETVHRLWDVFVGEDATLVEVNPLVRTEDGQIIALDGKVSLDDNAAFR
                     HPDHEALADRAAADPLEAKAKAKGLNYVKLDGQVGIIGNGAGLVMSTLDVVAYAGEEF
                     GGVKPANFLDIGGGASAEVMADGLEIILGDPSVRSVFVNVFGGITACDAVANGIVAAL
                     RILAGKGESLTKPLVVRLDGNNAAAGRAILAELGSNGSEADKALAAVIELVDTMDDAA
                     RRAAELAASAK"
     gene            complement(3432..3848)
                     /locus_tag="VLC50_03965"
     CDS             complement(3432..3848)
                     /locus_tag="VLC50_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018252374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HSF26655.1"
                     /translation="MASRPPLRIVVAKPGLDGHDRGAKVVARALRDAGMEVIYTGLHQ
                     TPEQIVETAIQEDADAVGLSVLSGAHMTLFARVIDLLRERGAADIAVFGGGIIPEADI
                     PELHRLGVAKVFTPGATTHEIVSWITSHVGGTTASA"
     gene            4045..5082
                     /locus_tag="VLC50_03970"
     CDS             4045..5082
                     /locus_tag="VLC50_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HSF26656.1"
                     /translation="MGPARVLSTLLRPRTVRGLAVEVAWVSAHLALYPLGVAQEQECA
                     ALGPFGRRRSLRGLTPLQRGLVVGDVEAAGTPILLVHGMVDNRSIFALLRRSLLRRGF
                     GRVATMNYRPAATDVREVARELALAVEALCAETGYERVHVVGHSLGGLVARYYVQRLG
                     GDERVHTLVTLGTPHHGSAPARLVPHPLMRQLRPGSALLTELAEPAVCRTRFVAVWSD
                     ADQMVLPWRSARIDHPDLAARNVLVPGVGHMSLPIDRRVVHEVSTTLAHLSTEGATVT
                     AGVSRLETGSGTSIVGASTVTQTAVPNRSAGLEPLNRRTTRGVTVTERGDTPCWSGEL
                     AGQDGAGWRSS"
     gene            5352..6092
                     /locus_tag="VLC50_03975"
     CDS             5352..6092
                     /locus_tag="VLC50_03975"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020388168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HSF26657.1"
                     /translation="MTSGVVGFAGSAPQGQADQPSRDTAAASVPLTADPVALAVADAV
                     AESRGQRVSRSLQRFELDIEAIEGAKAIDERVVAKAEEDAAEAARLEAERKAKAEAAR
                     KAAAKKKAAAARAAAAPRWVMPVSGARMSASFGEGGGLWANRHTGQDFAAPSGTPVRA
                     VGDGTIVSAGWDGAFGRKIVIRHRDGTQTWYCHLSSFTRTSGTVKAGQQIGRVGSTGN
                     TTGPHLHFEVHPGGGDPINPLSWLRART"
     gene            complement(6270..8627)
                     /gene="pcrA"
                     /locus_tag="VLC50_03980"
     CDS             complement(6270..8627)
                     /gene="pcrA"
                     /locus_tag="VLC50_03980"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018550530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HSF26658.1"
                     /translation="MGASEAAPRLDDEMSTLFDDVLPTPPDLPGHDGAGRERHSHGSD
                     PEALLTELNPQQRKAVVHEGRPLLVIAGAGSGKTRVLTHRIAYLMAARKVPPGAILAI
                     TFTNKAAGEMKERVAAHVGARAKAMWVSTFHSACVRILRAEHAALGFKSSFSIYDQAD
                     SVRLMALCCRDLDLDPKRYQPKGFAAKVSNLKNELVDHETYAAQVRDGSHDDQLLAEA
                     YQLYQQRLRQANAFDFDDLIMTTVNLLQAFPDVADHYHRRFRHILVDEYQDTNHAQYV
                     LVRELVGGGDPDAGSDGLAPAELAVVGDADQSIYAFRGASIRNILAFEKDYPDARTIL
                     LEQNYRSTQTILSAANAVIAKNPSRKPKNLWSDAGPGERLTGYVADNERDEAAYVAGE
                     IDELRDAGTTSYGDVAVFYRTNAQSRVFEEVFIRVGLPYRVVGGVRFYERKEIRDALA
                     YLRALANPDDTVSLRRILNVPKRGIGDRAQACLDALADRERISFPAAVARADEAIGLA
                     TRSLTQVRGFVELMDGLRTLVEAGTGPATVLEAVLEQTGYLDELAESADPQDETRAEN
                     LAELVAVAREFEETVPDGTLVDFLERVSLVADADSIPDEDEGVVTLMTLHTAKGLEFP
                     VVFLTGMEDGVFPHQRSLANTSELEEERRLAYVGITRAQRRLYLTRSLVRSAWGQPQY
                     WPASRFLAEVPDELIDWHGAVGGPATATAAPALARAANRPGARSPGNREIVHLEPGDR
                     VTHDTFGLGTVVSTAGVAERAEATIEFREVGLKRLLLRYAPVVKL"
     gene            8707..9432
                     /locus_tag="VLC50_03985"
     CDS             8707..9432
                     /locus_tag="VLC50_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018653661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HSF26659.1"
                     /translation="MLEDREVERALVVVAHPDDVDYGAGGTIAQWTDAGIEVTYCLVT
                     PGDAGGFDPTVDRADIPSIRMAEQRAAGEVLGVHDVRLLGYRDGQVEASYELRRDISR
                     VIRQVRPQRMLVQSPEWNFTRIGASHPDHMATGDATWRAIYPDARNPFAHPELISEEG
                     LADWKVAEVWMMSGPTPDVVIDVTATFDRKVAALRKHESQTDHMGEAFEEIVGGWLRD
                     NAAAHGLPQGHLAELFNVYTMPA"
     gene            9484..9951
                     /locus_tag="VLC50_03990"
     CDS             9484..9951
                     /locus_tag="VLC50_03990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF26660.1"
                     /translation="MKMRHIAMGSSWQRVRRSAAVALTFLCMSCSGGSGGGAAASCVG
                     PYLDDQPPSGVFGAPIPTVAPGARLIVYGHWYTSTCNDTGGQHDPLVPLPPVRLTLTL
                     PGGSAVDLGRFSPAGDDMGFSTAVQVPADSAAGVAVVRDDRGYGAPYTFQVGR"
     gene            complement(9969..10319)
                     /locus_tag="VLC50_03995"
     CDS             complement(9969..10319)
                     /locus_tag="VLC50_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018510510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF26661.1"
                     /translation="MDVVLRILLFGHFIGLASLLGGYITQLSLRNWRVGPAMVHGALT
                     QLVTGVAMVGILSATRDAADVNNAKFAVKLLVLLVILALVWANRSKTPAAPKVFQAIG
                     ALTVADIAIAVFWT"
     gene            complement(10512..11372)
                     /locus_tag="VLC50_04000"
     CDS             complement(10512..11372)
                     /locus_tag="VLC50_04000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF26662.1"
                     /translation="MAEHDPIADRLDALRGDVETLRLASPAAVRRRGDVRRARRTAVT
                     GVTALLVLAAGAGAAFGVLGGDRGTQQSVATPPTASSAPPTSPTPTPSPTAVLPLDPA
                     QAARAALLPADALDADATAEERFPDTDLNPCRYDNDQPHDVTLWQVISTAAGTEVHQR
                     VIVWPDVSVAKSHWNAVHNDLVACPAASGRYGPLQVSPMGPLAGGWAIELPSGAAPTQ
                     VYAAGFNVGRVTSEIYFTDPSAPAGAESVRQSQFHALADAAAQRLANAVHGNSAHDET
                     AATAPPSSSP"
BASE COUNT         1531 a         3961 c         4278 g         1646 t
ORIGIN      
        1 caggaacgcg agctgcgcgg ctggctctac cgcccggtcg ccgaccccga cgccacgctg
       61 gccgcggcgc tggagcgggt ggccgccgac gtcgaggacg gccacgacgt gagcgtcgag
      121 gtggtggtgg tcggcgacgc gaggctggac gagcggctgg ccgcgcttct gcaggcggcc
      181 cgggaggcgc tggtcaacgc cgccaaatac gccggggcgg ccgggccgat ctcggtgtac
      241 ggcgaggtcg aggacgggca ggtcaccgtg ttcgtgcgcg accgcggacc cgggtttgca
      301 ctggaggcgg tgccggccga ccggatgggc gtgcgcgagt cgatcctggg ccgcatgagt
      361 cgcaacggcg gccgggccgt cgtacggtca gagcccggtg ccggcaccga gatcgagctg
      421 gacatgcccc gggagggagc ctcgtgagcg gcgagcgcac ggtcagggtg gtcatcgtcg
      481 acgaccaccg gatgtttcgc accggggtgc gcgccgagct ggggcaacgg gtcgacgtgg
      541 tcggcgaggc cgacgacgtc gacaccgccg tcgccgtgat cagcgagacc cggcccgacg
      601 tcgtgctcct tgacgtgcac ctgcccggcg gtggtggacg tgaggtgctg cgccggtgcg
      661 tgccgagcct gccggacacc acgttcctcg cgctgtcggt ctccgacgcg gccgaggatg
      721 tcatcggggt gatccgggcc ggcgcccgcg gctacgtcac caagtcgatc actggcgacg
      781 agctggctgc ggcgatccgg cgggtggccg acggcgacgc ggtgttctcg ccgcggctgg
      841 ccggcttcgt gctcgacgcg ttcgccggcg cggtggtgcc ggccgacgtc gacgaggacc
      901 tcgaccggct caccgcccgc gagcgggagg tgctgcggct gatcgcgcgc ggctacgcct
      961 acaaggaggt cggcaagcag ctgttcatct cggtcaagac ggtggagacg cacgtgtccg
     1021 ccgtcctgcg caagctgcag ctgtccaacc ggcacgagct gacccgctgg gccaccgacc
     1081 gccgcctcgt ctgatctctt actcgcccag ccccccgtta cgcgctgagg ccaccggatc
     1141 ggcgcgcgat ccggtggcct cggcgagtaa tgcgggcgtg ggcgagtaag cctcagcggg
     1201 cggtgacgag ctcgcgcatc agccgggcgg tctcgctggg cgtcttgccg acgcgcacgc
     1261 cgaccgcctc gagggccgcc ttcttggccg cggcggtgcc cgacgagccg gacacgatgg
     1321 cgccggcgtg gcccatggtc ttgccctcgg gggcggtgaa cccggcgacg tagccgacca
     1381 ccggcttgct cacgtgctcc gcgatgtagg cggcggcccg ctcctcggcg tcgccgccga
     1441 tctcaccgat catcacgatc gcgtcggtgt cggggtcgtc ttcgaacgcc tgcaggcagt
     1501 cgatgtgggt ggtcccgatg accgggtcgc cgccgatgcc cacggcggtg gagaagccga
     1561 ggtcggccag ctcgtacatc atctggtagg tcagcgtgcc cgacttggac accagcccga
     1621 tccggccggc cttggcgatc gtggccggga tgatgcccac gttggacttg cctgggctga
     1681 tcaggcccgg gcagttgggg ccgatcagcc gggtggtgcc ggagttggcg gcgtactggt
     1741 agaacgccgc gctgtcgtgc accggcacgc cctcggtgat caccacgacg agcgggatgc
     1801 ccgcgtcgac ggcctcgtag acggcggcct tggtgaacgc cggcggcacg aacacgaccg
     1861 acacgtcggc gccggcctcc ttgaccgcgt cggtgacggt gccgaagacc ggcagcgtcg
     1921 cgccgtcgaa gtcgagggtc tgcccggcct tcttggggtt ggtgcccccg acgatcgtgg
     1981 tgccggacgc gagcatccgc tgggtgtgcg cccgcccctg cgagccggtc atgccctgga
     2041 cgacgacccg gctcttctcg gtgaggaaga tggccatagt tcttctcttc cctacttcgc
     2101 cgaggcggcg agctcggccg cgcggcgcgc ggcgtcatcc atcgtgtcca ccagctcgat
     2161 caccgcggcg agcgccttgt cggcctcgga cccgttggag ccgagctcgg ccaggatcgc
     2221 ccggccggcg gcggcattgt tgccatcgag ccgtacgacg agcggcttgg tcagcgactc
     2281 gcccttgccg gccaggatcc gcagcgccgc cacgatgccg ttggccaccg cgtcgcaggc
     2341 ggtgatgccg ccgaagacgt tgacgaacac cgagcgcacc gaggggtcgc ccaggatgat
     2401 ctcgagcccg tcggccatca cctcggccga ggccccgccg ccgatgtcga ggaagttggc
     2461 cggcttgacc ccgccgaact cctcgccggc gtaggccacc acgtcgagcg tggacatgac
     2521 cagcccggca ccgttgccga tgatgccgac ctgcccgtcg agcttgacgt agttgaggcc
     2581 cttggccttg gccttggcct ccagcgggtc ggccgcggcc cggtcggcca gcgcctcgtg
     2641 gtcggggtga cggaacgcgg cgttgtcatc gaggctgacc ttgccgtcga gcgcgatgat
     2701 ctgcccgtcc tcggtgcgca cgagcgggtt gacctcgacc agcgtggcgt cctcgccgac
     2761 gaagacgtcc cacagccggt gcacggtctc gacgacctgg tcggcgacct cggcggggaa
     2821 cccgccggcg gccacgatct cgcgggcctt ggcctcgtcg atgccggcga tcgcgtcgac
     2881 cggcacccgg gccagcgcct cgggcttctc gaccgcgagc acctcgatct ccatgccgcc
     2941 ctcgcgggag gccatcgcga ggtaggtgcg gttggcccgg tcgagcagga tcgagaagta
     3001 gtactcctgg gcgatcttgg cgccgggcgc caccatcacc cggtgcacgg tgtggccctt
     3061 gatgtccatg ccgaggatcg cctcggccac ggccgcggcc tcgtcggggg agtgggccag
     3121 cttgaccccg ccggccttgc cgcgcccacc ggtcttgacc tgggccttca ccaccacggt
     3181 gccgccgccg agggactgcg cggccgcgcg ggccgcctcg ggggtctcgg ccaccgctgc
     3241 gtcgagcacg ggtacgccgt gctgggcgaa gaggtccctc gcctggtact cgaaaagatc
     3301 cacgggctcg cgtcctccgc atcacagggc tgtcgtggcc gtcgaggcac agcgacggcg
     3361 tgcgtgcggc ggccgatccg ctccgagggt atcggcgccg gatcgggtgg caccaaccgg
     3421 gcagagccgg ctcaggccga ggcggtcgtc ccgccgacgt ggctggtgat ccagctgacg
     3481 atctcgtggg tggtcgcccc gggcgtgaag accttggcca cgccgagccg gtggagctcg
     3541 gggatgtcgg cctcggggat gatgcccccg ccgaacaccg cgatgtcggc ggcgccacgc
     3601 tcgcgcagca ggtcgatgac ccgggcgaac agcgtcatgt gagccccgga gagcaccgag
     3661 aggccgaccg cgtcggcgtc ctcctggatc gccgtctcga cgatctgctc gggggtctgg
     3721 tgcagcccgg tgtagatgac ctccatgccg gcgtcgcgca gggcgcgcgc gaccaccttt
     3781 gcgccgcggt cgtgcccgtc gagtcccggc ttggcgacga cgatgcgcag cggcgggcgg
     3841 gacgccatga cgggactcct tcgcgggcgg atgtcgctca cgcgacgcga gcgcaacgga
     3901 cgcgggcgca acgactgtgt caccgcagcg tatcggccgt gccacccgag gtgtccggga
     3961 gagacgtgcg gggtaggcca cggggtggcg cgcgggcgcg tcgtcgtacg catcgtgccg
     4021 tgaggaggcc cccgggtgag cgagatgggg ccggcgcgcg tcctgtcgac cctgctgcgc
     4081 ccccggaccg tgcgagggct ggccgtcgag gtcgcctggg tcagcgccca cctcgcgctc
     4141 tacccgctcg gggtggccca ggagcaggag tgcgccgcgc tcgggccgtt cgggcgccgc
     4201 cgcagcctgc gcgggttgac cccgctgcag cggggcctcg tggtcggtga cgtcgaggcc
     4261 gcgggcaccc cgatcctgct ggtccacggg atggtcgaca accggtcgat cttcgcgctg
     4321 ctgcgccgca gcctgctccg ccgcggcttc ggccgggtcg cgacgatgaa ctaccgaccg
     4381 gcggccaccg acgtgcgaga ggtggcccgc gagctggcgc tggcggtcga ggcgctgtgc
     4441 gccgagaccg gctacgagcg ggtgcacgtg gtgggccaca gcctcggcgg gctggtcgcc
     4501 cgctactacg tgcagcggct cgggggcgac gagcgggtgc acacgctggt cacgcttggc
     4561 acgccgcacc acggcagcgc ccccgcccgc ctggtgcccc acccgctgat gcgccagctg
     4621 cgcccgggca gcgccctgct caccgagctg gccgaaccgg ccgtctgccg gacccggttc
     4681 gtggcggtgt ggagcgacgc cgaccagatg gtgctgccct ggcgcagcgc gcggatcgac
     4741 cacccggacc tggccgcccg caacgtgctc gtcccgggcg tggggcacat gtcgctgccg
     4801 atcgaccggc gggtggtcca cgaggtgtcg accacgctgg cccatctgag caccgaaggc
     4861 gcaaccgtca ccgcaggtgt cagtcggctc gaaacgggta gcggtacgtc gatagtgggc
     4921 gcttcgacgg tcacccagac cgcggtgccc aaccgatcgg ccggcctcga gccgctgaat
     4981 cggcgtacga cgcgtggtgt gaccgtcact gagcgtggcg acacgccgtg ctggagcgga
     5041 gagctcgccg ggcaggacgg cgcgggctgg cgctcgtcgt aaccggttcg ttatggtctc
     5101 ggggtcctgg tccctaacac cctggtatct ccgtgtccac accgcttcat ccgctcaccg
     5161 acgtcgatga ccctgatgac ctcattgcag gtccgctgag ccagtcgagc gccgagccgc
     5221 ggggcggccg tcaccggcgg cgcaccgcgc ccgctccggc gccgcgcgaa ggcacccagc
     5281 ggtcgggccg gcggcgggcg aggcggcgac agaggaccgc ccggctgggg gccgcgatcg
     5341 cggtgatggc gatgaccagc ggcgtcgtgg gattcgccgg gtcggcgccg caggggcagg
     5401 ccgaccagcc gagccgggac acggccgcgg catcggtccc gctcaccgcc gatccggtcg
     5461 ccctcgcggt ggccgacgcg gtcgccgagt cgcggggtca gcgcgtgtcc cggtcgctgc
     5521 agcgcttcga gctcgacatc gaggcgatcg agggcgccaa ggcgatcgac gagcgcgtgg
     5581 tcgccaaggc cgaggaggac gccgccgagg cggcgcgtct cgaggccgag cgcaaggcca
     5641 aggctgaggc cgcccgcaag gcggccgcga agaagaaggc ggcggccgcc cgagcggcgg
     5701 cggcgccccg gtgggtgatg ccggtcagcg gcgcccgcat gtcggcgtcc ttcggcgagg
     5761 gcggcgggct gtgggccaac cggcacaccg gtcaggactt cgcggctccc agcggcaccc
     5821 cggtacgcgc cgtcggtgac ggcacgatcg tctcggccgg ctgggacggc gccttcggac
     5881 gcaagatcgt gatccgccac cgcgacggca cgcagacgtg gtactgccac ctgtcgtcgt
     5941 tcacccggac cagcggcacg gtcaaggccg gccagcagat cggccgggtc ggcagcaccg
     6001 gcaacaccac cgggccgcac ctgcacttcg aggtgcaccc cggcggcggc gacccgatca
     6061 acccgctgtc ctggctgcgc gcccggacct gagttccgac cgccggcgct gctgctgccc
     6121 ggctgcatcg ctcgcgggat cttgagacgc ctacgtcgtg gtcgggtccg ctgcggccgg
     6181 accgtgcgac tgtgcagcgt cccgacgacc acaaagtcgc acaatcacgt gatcatgcac
     6241 tcgtgcgggg gccgacgccg agcaggggct taaagcttga cgacgggggc gtagcgcagc
     6301 agcagccgct tgagccccac ctcgcggaac tcgatcgtcg cctcggcccg ctcggcgacg
     6361 ccggcggtcg agacgaccgt gccgagcccg aaggtgtcgt gggtgacccg gtcgccgggc
     6421 tccaggtgga cgatctcgcg attgcccggc gagcgggctc cgggacggtt ggcggcccgg
     6481 gcgagcgccg gcgccgccgt ggcggtggcc ggcccgccga ccgcgccgtg ccagtcgatg
     6541 agctcgtcgg gcacctcggc gaggaagcgt gaggccggcc agtactgcgg ctggccccag
     6601 gccgagcgga cgagcgagcg ggtgaggtag agccggcgct gggcacgggt gatgccgacg
     6661 taggccagcc ggcgctcctc ctccagctcg ctggtgttgg ccagcgaccg ctggtgcggg
     6721 aagaccccgt cctccatgcc ggtgaggaag accactggga actccagtcc cttggcggtg
     6781 tgcagggtca tcagcgtgac gacgccttcg tcctcgtcgg ggatcgagtc ggcgtcggcg
     6841 accagcgaga cccgctcgag gaagtcgacc agggtgccgt cgggcacggt ctcctcgaac
     6901 tcgcgggcca cggcgaccag ctcggcgagg ttttccgcgc gggtctcgtc ctgcgggtcg
     6961 gccgactcgg ccagctcgtc gaggtagccg gtctgctcga ggaccgcttc gagcacggtc
     7021 gccggcccgg tgccggcctc gaccagcgtg cgcagcccgt ccatgagctc gacgaagccg
     7081 cggacctggg tcagcgaccg ggtggccagc ccgatcgcct cgtcggcgcg ggccaccgcg
     7141 gcggggaacg agatgcgctc gcggtcggcc agcgcgtcga ggcaggcctg ggcccggtcg
     7201 ccgatgccgc gcttgggcac gttgaggatc cggcgcaacg agacggtgtc gtcggggttg
     7261 gccagcgcgc gcaggtaggc cagcgcgtcg cggatctcct tgcgctcgta gaagcgcacg
     7321 ccgccgacca cccggtaggg cagcccgacc cggatgaaca cctcctcgaa cacccgggac
     7381 tgggcgttgg tgcggtagaa gacggcgaca tcgccgtacg acgtcgtgcc ggcatcgcgc
     7441 agctcgtcga tctcgccggc gacgtaggcc gcctcgtcgc gctcgttgtc ggcgacgtag
     7501 ccggtcagcc gctcgccggg acccgcatcg gaccagaggt tcttgggctt gcgcgagggg
     7561 ttcttggcga tgacggcgtt ggccgcggac aggatcgtct gggtcgagcg gtagttctgc
     7621 tcgagcagga tcgtgcgcgc gtcggggtag tccttctcga aggccaggat gttgcggatc
     7681 gacgcaccgc ggaaggcgta gatcgactgg tcggcatcac cgaccacggc cagctcggcc
     7741 ggtgccagcc cgtcgctgcc ggcatccggg tcgccgccac ccaccagctc gcgcaccagc
     7801 acgtactgcg cgtggttggt gtcctgatac tcgtcgacca ggatgtggcg gaaccgccgg
     7861 tggtagtggt cggcgacgtc ggggaacgcc tgcagcaggt tgaccgtcgt catgatcagg
     7921 tcgtcgaagt cgaaggcgtt ggcctgccgc agccgctgct ggtagagctg gtaggcctcg
     7981 gcgagcagct ggtcgtcgtg gctgccgtca cggacctgcg cggcgtaggt ctcgtggtcg
     8041 accagctcgt tcttgaggtt ggacaccttg gcggcgaagc ccttgggctg gtagcgcttg
     8101 gggtcgaggt cgaggtcgcg gcagcacagg gccatcagcc gcaccgagtc ggcctggtcg
     8161 tagatcgaga agctgctctt gaacccgagc gcggcgtgct cggcccgcag gatgcgcacg
     8221 caggcggagt gaaacgtgga cacccacatc gccttggccc gggccccgac gtgggcggcc
     8281 acccgctcct tcatctcgcc ggcggccttg ttggtgaacg tgatcgcgag gatcgcgccc
     8341 ggaggcacct tgcgcgcggc catgaggtag gcgatgcggt gggtgagcac gcgggtcttg
     8401 cccgacccgg cgccggcgat gacgagcaac gggcggccct cgtggacgac cgccttgcgc
     8461 tgctgcgggt tgagctcggt cagcagggcc tcgggatcgc tgccgtgcga gtggcgctcg
     8521 cgccccgcgc cgtcgtgccc aggcaggtcg ggcggggtgg gcaggacgtc gtcgaagagg
     8581 gtgctcatct cgtcgtcgag tctaggcgcc gcctccgacg cgcccacccg agcaacggtg
     8641 cacggtcctg atccgtacgg cgtgggcgtt agcgtgccgc cgtacccctt ggaagggaga
     8701 tcgaccgtgc tggaggaccg cgaggtggag cgggcgctcg tcgtcgtcgc ccaccccgac
     8761 gacgtcgact acggcgccgg cggcacgatc gcgcagtgga ccgacgccgg catcgaggtg
     8821 acctactgtc tggtcacacc cggtgacgcc ggtggtttcg acccgacggt cgaccgtgcc
     8881 gacatcccga gcatccggat ggccgagcag cgcgcggcgg gtgaggtgct gggcgtgcac
     8941 gacgtacgac tgctgggcta tcgcgacggg caggtggagg cgtcctacga gctgcgccgt
     9001 gacatctccc gggtgatccg ccaggtccgg ccgcagcgga tgctcgtgca gagcccggag
     9061 tggaacttca cccgcatcgg ggccagccac cccgaccaca tggccaccgg cgacgccacc
     9121 tggcgggcga tctaccccga cgcgcgcaac ccgttcgccc accccgagct gatcagcgag
     9181 gaggggctgg ccgactggaa ggtcgccgag gtctggatga tgagcgggcc gacccccgac
     9241 gtggtcatcg acgtgaccgc gacgtttgac cgcaaggtcg cggcgctgcg gaagcacgag
     9301 agccagaccg accacatggg cgaggcgttc gaggagatcg tcggcggctg gctgcgcgac
     9361 aacgccgccg cgcacgggct gccgcagggt cacctggccg agctgttcaa cgtctacacg
     9421 atgccggcct gagcggttct ctgggtcgcc gtgacgtgac cggtggtcgc ggaaccagga
     9481 ccggtgaaaa tgcgtcacat cgccatggga tcttcgtggc aacgcgtgcg gcgctccgcg
     9541 gccgttgcgc tgacgttcct gtgcatgtcc tgcagtggcg gctcgggcgg cggcgcagcc
     9601 gcgtcctgcg ttggtcccta tctcgacgat cagccgccga gcggagtctt cggtgcgccg
     9661 atccccaccg tcgccccggg tgcgcgcctc atcgtctacg gccactggta cacgagcacc
     9721 tgcaacgaca cgggcggaca acacgaccca ctggtgccgc tgcctccggt gcggttgacc
     9781 ctcacgctgc ctggcgggtc ggctgtggac ctgggccggt tcagcccggc cggtgacgac
     9841 atgggtttct cgaccgctgt ccaggtgccg gcggactcag ccgcaggggt cgccgtggtt
     9901 cgcgacgacc gcggttacgg ggcgccgtac accttccagg tcgggcggta gcccgatacc
     9961 ccggtggctc aggtccagaa caccgcgatc gcgatgtcgg ccacggtcag cgcaccgatc
    10021 gcctggaaca ccttgggcgc cgcgggtgtc ttgctgcggt tggcccagac cagcgcgagg
    10081 atcacgagca gtacgagcag cttgacggcg aacttggcgt tgttgacgtc ggcggcgtcg
    10141 cgggtggccg agaggatgcc gaccatcgcg accccggtga ccagctgggt cagcgcgccg
    10201 tgcaccatcg ccggcccgac ccgccagttg cgcaggctca gctgggtgat gtagccgccg
    10261 agcaggctgg ccagcccgat gaagtggccg aacagcagga tgcgcagtac gacgtccatg
    10321 tccagatgat ggccgacgtg tcccgcatcg cggccaccgg gtcacggctc agcgcagccg
    10381 cagggccgcg tgatcatgca ctcgtgcggc acggtgagga cttggcccag cagccatgat
    10441 cgtgtcaata cgcagcccag gagcccatgc gatcttccac gatcacgcca gagccgtgca
    10501 tacgtgcatg atcacgggga gctgctgggc ggagcggttg ccgcagtctc gtcgtgtgcg
    10561 ctgttgccat gtacggcgtt ggccagtcgt tgcgccgcgg cgtcggcgag cgcgtggaac
    10621 tggctctgcc gcacactctc ggcaccggcc ggggccgacg ggtcggtgaa gtagatctcg
    10681 ctggtcaccc ggcccacgtt gaacccggcg gcgtagacct gcgtcggcgc ggcgccggag
    10741 ggcagctcga tcgcccagcc gccggccaac ggccccatcg gagacacctg caacgggccg
    10801 tagcggccgg acgccgcggg gcaggccacc aggtcgttgt gcacggcgtt ccagtgcgac
    10861 ttggccaccg agacatcggg ccagacgatg acccgctggt gcacctcggt ccctgcggcc
    10921 gtgctgatca cctgccagag cgtcacgtcg tgcggctggt cgttgtcgta gcggcacggg
    10981 ttgaggtcgg tgtcggggaa gcgctcctcc gcggtggcgt cggcatccag cgcgtcggcg
    11041 ggcagcagcg ccgcgcgggc ggcctgcgcc gggtccagcg ggagcaccgc cgtcggtgac
    11101 ggcgtcgggg tgggcgaggt cggcggggcc gaagacgcag tcggcggggt cgccaccgac
    11161 tgctgcgtgc cgcggtcacc cccgagcacc ccgaaggcgg caccggcacc ggcggcgagc
    11221 acgagcaggg cggtcacgcc ggtcacggca gtccgccggg cccgtcgtac gtcgccgcgc
    11281 ctgcgcaccg ccgccgggct ggctagccgc agcgtctcga cgtcgccgcg cagcgcgtcg
    11341 agccggtcgg cgatcgggtc gtgctcagcc acgggggtcc tcctcgacga tgagtgcggc
    11401 cagggcggcc cgcccg
//
LOCUS       DATIBB010000104         7678 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_1766428, whole genome shotgun sequence.
ACCESSION   DATIBB010000104 DATIBB010000000
VERSION     DATIBB010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 7678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7678
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1766428"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(98..1813)
                     /locus_tag="VLC50_04005"
     CDS             complement(98..1813)
                     /locus_tag="VLC50_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005465006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HSF26663.1"
                     /translation="MTAATESFRAARDLLIRHREDYDTARAEFTWPVLPEFNWALEWF
                     DEIARDNDAPALWIVEDDGSQTQVSFATMADRSGQLAAWLQAQGVDRGDRIVLMLGNQ
                     VELWETILAAMKLGAVIIPATPMLGPADLRDRIERGNAKHVVTTSADAAKFADVPGSY
                     TRIAVGEPVPGWLRYADSTSWLESFTPEVPTRSDETMLLYFTSGTTAKPKLVEHTHAS
                     YPIGHLSTMYWIGLRPGDVHLNISSPGWAKHAWSNVFAPWNAQACVLIANFARFDAAT
                     LLAVMRDCGVTTFCAPPTVWRMLIQADLAANAVPTLRECVGAGEPLNPEVIEQVRKAW
                     GITIRDGYGQTETTAQVGNPPGAPLKAGSMGRPLPGFEVTLLDPLTSEPADEGEICLD
                     LSARPVGLMAGYRDDDARNAEATLGGYYHTGDVGRVDADGYITYVGRADDVFKASDYR
                     ISPFELESVLIEHPAVAEAAVVPSPDPQRLAVPKAYVVLAAGHDPSKETAESILRYAR
                     EHLAPYKRVRVIEFAELPKTISGKIRRVELRGRERDAHGAGTDRDLRASGTEFWEDDF
                     PDLKA"
     gene            complement(1810..2790)
                     /locus_tag="VLC50_04010"
     CDS             complement(1810..2790)
                     /locus_tag="VLC50_04010"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HSF26664.1"
                     /translation="MGAVPVVVVGDTLAELVVPVTEAGSPTEDPRALVVRPHGAAART
                     AVWLASLGIPTTLVGRIGEDALGRAIAADLARRRVDVVLRAVPGAPTGTVVTLTSPYG
                     EAARVDEPGAAAMLSPDDLPADTFVAGTHLHLAGRALVDPSGSGVGAAAIRLARTAGM
                     TVSAEPPPVPVLQALDPATARTALSGVDLLVTGVAEAFALVEDVLEQEGGDLRPERYA
                     AALARSVAAVVVTGGPAGAWWHGRRSAPAHVMVHGDADRGTGDVSLDPFTDDGTRDDV
                     RPAFVAGLLAAWLLDARPVQALRSGCTIATRARGGPDPLPDEGGGARVRT"
     gene            2827..5469
                     /locus_tag="VLC50_04015"
     CDS             2827..5469
                     /locus_tag="VLC50_04015"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016188982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HSF26665.1"
                     /translation="MQSLYPAHWEADVVLSDGATAHLRPITPEDADRLVLFYERVSDQ
                     SKYYRFFAPYPRLTAQDVDRFTHVDHDARVAFIVVVGEDMIAVGRYDRVGRDTAEVAF
                     LVEDAHQGRGLGSVLLEHLAAAARERGVRRFVAEVLPANRRMLTVFQDAGYSVEHELD
                     EGIVSLTFDIEPTVHSIEVMASREHRAEARSIEALLRPRRIAVVGASRTYATVGQSIL
                     RHLVEAGFTGEVVAVNPEADQVAGIPAYPAVRDVPGPLDLAVVAVPAGEVDRVVTDCA
                     RKGVRGLVVVSAGFAETGEAGRDRQRRLVRTARANGMRVVGPNSFGMINTDPAVRLNA
                     SLAPTMPTRGRIGFFSQSGALGIAILEAVSGRGLGLSTFVSAGNRADVSGNDLLQYWE
                     EDPDTDVVMLYLESVGNPRKFSRLARRVARSKPVAVVKSGTSAQMVPLGHAVRRSKAP
                     AAALDAMFRQSGVIRVETIHQLFDVAALLATQPLPTGDRVAILGNSDALGLLAAGAAE
                     AWGLHVVGEPLALGAEATAEDFRRALAAVFADPAVDSVVAVFIPPLLTLDEDVASVLA
                     EAAARATKTVVSTFLGMRGVPAALRAGSDGRGAVPSYPTPEDAVRALVAATRYAQWRR
                     RPIGSVPALPDVDVAAARQLVEQVLAECPDGRALTRDETAALLLPFGVKIWPAVPVHD
                     IDSAVAAAEALGYPVALKATARALRHRIDLGGIHLGLADEADLRQAYAQLVRLQADDD
                     LGLVVQPMAGPGLAVVVSSAEDPLLGPYLAFGIGGVVSELLGDLAYRIPPLTDVDAAE
                     MVRSVRAAPMLFGHRGAEPVDVAAVEDLLLRVSRLADELPEVASLELNPVVVGAHGLV
                     VLAAEASVAHPAVRTDSPRRSLPG"
     gene            5513..6115
                     /locus_tag="VLC50_04020"
     CDS             5513..6115
                     /locus_tag="VLC50_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019890001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5998 family protein"
                     /protein_id="HSF26666.1"
                     /translation="MPETRSTERTRPSLQDSIERTGYFPQVVADAVEDALADEPVTAH
                     LVHQETTFDSDEVRRHVTVVALTASRLLVAHTDDHPAGADTPTALATTSVEVVPLDAL
                     RSVIVNRVVANPATYVRGADPQEVVLTVGWGAVNRIDVEPAGCSDPSCEADHGYTGTS
                     GADDLSLRVSSAAEGPDIVAEALALGRALSRALARSGGQR"
     gene            6118..7293
                     /locus_tag="VLC50_04025"
     CDS             6118..7293
                     /locus_tag="VLC50_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020555836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HSF26667.1"
                     /translation="MRGPSGDPAAATDWRAAAARLRPPYGEASLADLLPSVCAHLGVP
                     GCSDVLGLPATDRACVLLVDALGWHLLRRHPETAPLLHELAEEGRLITAGFPTTTPTS
                     LACLGTGLAPGGHGLVGFQVLVPGPERVMNLLTWDEGVDPVAFQPSATLMQRAEASGV
                     TVTTVGPRAYRGTGLTVAALRGGVHRGGDSIGELVAGITAALAEPGPALVYAYYPGLD
                     QTGHRNGCDSPAWLAELSVVDHMVGLLREALPSGAVLYVTGDHGMVDVPLDRRIDMAT
                     EPALREGVALVAGEPRVVYLRTVCGATSDVMATWRDRLGSDFWVLPTEEVIELGWYGA
                     VRDGVAARIGDIVVAARVPAVSIVDSERTPRGLLRLKALHGSLTDHERHVPLLTLRG"
     gene            complement(7368..>7678)
                     /gene="sepH"
                     /locus_tag="VLC50_04030"
     CDS             complement(7368..>7678)
                     /gene="sepH"
                     /locus_tag="VLC50_04030"
                     /inference="COORDINATES: protein motif:HMM:NF040712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="septation protein SepH"
                     /protein_id="HSF26668.1"
                     /translation="LVYDVEADGGLDRIGEPLRLRRAPGDHLAPVISVSEAREEAARG
                     AAAEAAEEQAQSTEAAEPPQAAPAGPEPTSAPGARRRTGRRASVPSWDDILLGTRRQP
                     "
BASE COUNT          915 a         2864 c         2844 g         1055 t
ORIGIN      
        1 cagcagggca cggatcaccg cggccggcag cggccggtcc gggtcgtagg cccgcaccat
       61 ccgccggcgt cgtacgacgt cgtcgagctc caccggctca ggccttcaga tcggggaagt
      121 cgtcctccca gaactccgtg ccgctggccc gcaggtcgcg gtccgtgccg gcgccgtggg
      181 cgtccctctc gcgcccgcgc agctcgaccc ggcggatctt gccgctgatc gtcttgggca
      241 gctccgcgaa ctcgatgacc cgcacgcgct tgtagggcgc caggtgctcc cgggcgtagc
      301 gcaggatcga ctcggcggtc tccttgctcg ggtcgtgccc ggccgcgagc acgacgtagg
      361 ccttgggcac ggccaggcgc tgcgggtcgg gggagggtac gacggccgcc tcggccaccg
      421 ccgggtgctc gatcagcacg ctctccagct cgaacggcga gatccggtag tccgacgcct
      481 tgaacacgtc gtcggcgcgc ccgacgtagg tgatgtagcc gtcggcgtcg acccgcccga
      541 cgtcgccggt gtggtagtag ccgcccaggg tggcctcggc gttgcgcgcg tcgtcgtcgc
      601 ggtagccggc catcagcccg accgggcggg ccgacaggtc gaggcagatc tcgccttcgt
      661 cggccggctc gctggtcagc gggtcaagca gcgtgacctc gaagccgggc agcggccggc
      721 ccatcgatcc cgccttgagc ggcgccccgg gcgggttgcc cacctgcgcg gtggtctcgg
      781 tctgcccgta gccgtcgcgg atcgtgatgc cccaggcctt gcgcacctgt tcgatgacct
      841 cggggttgag cggctcgccg gcgccgacgc actcgcgcag ggtgggcacc gcgttggccg
      901 ccaggtcggc ctggatcagc atgcgccaca cggtcggggg agcgcagaac gtcgtgaccc
      961 cgcagtcgcg catcaccgcg agcaacgtgg ccgcgtcgaa gcgggcgaag ttggcgatga
     1021 gcacgcacgc ctgcgcgttc cacggcgcga acacgttgct ccaggcgtgc ttggcccagc
     1081 cgggcgagga gatgttgagg tgcacgtcgc ccgggcgcag cccgatccag tacatcgtcg
     1141 acaggtgccc gatcgggtag gacgcgtggg tgtgctcgac cagcttgggc ttggccgtgg
     1201 tgccgctggt gaagtagagc agcatcgtct cgtcggagcg ggtgggcacc tcgggggtga
     1261 acgactcgag ccaggaggtc gagtcggcgt agcgcagcca gccgggcacc ggctcgccca
     1321 ccgcgatgcg ggtgtagctg cccggcacgt cggcgaactt cgccgcgtcg gccgaggtgg
     1381 tcaccacgtg cttggcgttg ccgcgctcga tccggtcgcg caggtcggcc gggcccagca
     1441 tcggcgtggc cgggatgatc accgcgccga gcttcatcgc ggccaggatg gtctcccaca
     1501 gctcgacctg gttgccgagc atgagcacga tccggtcgcc ccggtcgacg ccctgggcct
     1561 gcagccaggc ggccagctgt ccggaccggt ccgccatcgt cgcgaacgac acctgggtct
     1621 gcgacccgtc gtcctcgacg atccacagcg ccggtgcgtc gttgtcacgg gcgatctcgt
     1681 cgaaccactc cagcgcccag ttgaactcgg gcagcaccgg ccaggtgaac tcggcgcgcg
     1741 cggtgtcgta gtcctcccgg tggcggatga gcaggtcgcg ggcggcccgg aacgactcag
     1801 tggcagctgt catgtccgca ccctggcacc gcccccctcg tcgggcaacg gatctgggcc
     1861 gcctcgggcc cgtgtcgcga tcgtgcagcc gctgcgcagg gcctggaccg ggcgggcgtc
     1921 gagcaaccag gccgcgagca ggcccgcgac aaaagcgggt cgcacgtcgt ctcgggtgcc
     1981 gtcgtcggtg aacgggtcga gcgagacgtc acccgtgccc cggtctgcgt ccccatgcac
     2041 catgacgtgc gcgggcgcgc tcctgcggcc gtgccaccag gctccggccg ggccgcccgt
     2101 gacgaccacc gccgcgacgg agcgggccag cgctgccgcg tagcgctccg gtcgcaggtc
     2161 gccgccctcc tgctccagga cgtcctcgac cagcgcgaac gcctcggcca caccggtcac
     2221 cagcaggtcg accccggaga gcgcggtccg tgcggtggcg gggtcgagcg cctgcagcac
     2281 cggcaccggt ggcggctcgg ccgagaccgt catcccggcc gtgcgggcga gccggatggc
     2341 cgcggcgccg acccccgatc cggaagggtc gaccagcgca cggccggcca ggtgcaggtg
     2401 ggtcccggcg acgaacgtgt cggccgggag gtcgtccggg ctcagcatgg cggccgcccc
     2461 cggctcgtcg acccgcgctg cttcgccgta cggcgaggtg agggtgacga cggtcccggt
     2521 gggcgcaccc ggcaccgccc gcagcactac gtcgacccgg cggcgcgcca ggtctgcggc
     2581 gatcgcgcgc ccgagcgcgt cctcgccgat ccggccgacc agcgtggtcg ggatgccgag
     2641 gctggccagc cagaccgcgg tgcgagccgc cgcgccgtgc ggccggacga cgagtgcgcg
     2701 cgggtcctcc gtcggcgacc ccgcctccgt caccggcacg accagctcgg ccagcgtgtc
     2761 cccgaccacg acgaccggca ccgcgcccac cccgcgaccg tagcgcgcgc cgatagcctc
     2821 gcaggcgtgc agagccttta ccccgcgcac tgggaggccg acgtcgtcct ctccgacggg
     2881 gccacggcgc acctgcggcc gatcacgccc gaggacgccg accggctggt gctcttctac
     2941 gagcgggtct ccgaccagtc gaagtactac cgcttcttcg cgccctaccc gcggctgacc
     3001 gcccaggacg tcgaccggtt cacccacgtc gaccacgacg cccgggtcgc gttcatcgtg
     3061 gtcgtcggcg aggacatgat cgcggtcggg cgctatgacc gggtgggccg ggacacggcg
     3121 gaagtggcct tcctcgtcga ggacgcccac cagggccgcg gcctcgggtc ggtgctgctc
     3181 gagcacctgg cagcggccgc tcgcgagcgc ggcgtacgac ggttcgtcgc cgaggtgctg
     3241 ccggccaacc gccggatgct caccgtcttc caggacgccg gctactcggt cgagcacgag
     3301 ctcgacgagg gcatcgtcag cctcaccttc gacatcgagc cgaccgtgca ctcgatcgag
     3361 gtgatggcct cgcgggagca ccgcgccgag gcccgctcca tcgaggcgct gctgcggcct
     3421 cgtcggatcg cggtggtcgg ggccagccgc acctacgcca cggtgggcca gtcgatcctg
     3481 cgccacctgg tcgaggccgg gttcaccggc gaggtcgtgg ccgtcaaccc cgaggccgac
     3541 caggtggccg gcatcccggc ctacccggcg gtgcgtgacg tgcccggccc gctcgacctg
     3601 gcggtggtgg ccgtgccggc cggtgaggtc gaccgggtcg tcaccgactg cgcccgcaag
     3661 ggggtgcgcg ggctcgtcgt cgtcagcgcg ggcttcgccg agaccggtga ggccggccgc
     3721 gaccgtcagc gccgcctcgt gcgcaccgcc cgggccaacg ggatgcgggt ggtcggaccc
     3781 aactcgttcg gcatgatcaa cacggatccg gcggtccggc tcaacgcgtc gctggcgccg
     3841 accatgccga cccggggccg catcggattc ttctcgcagt ccggggcgct gggcatcgcg
     3901 atcctcgagg cggtcagcgg tcgggggctg ggcctgtcga cgttcgtctc ggccggcaac
     3961 cgggccgacg tgtcgggcaa cgacctgctg cagtactggg aggaggaccc cgacaccgac
     4021 gtcgtcatgc tctacctcga gtcggtcggc aacccgcgca agttctcccg gctggcccgg
     4081 cgggtggcgc gcagcaagcc ggtggcggtg gtcaagtccg gcaccagcgc gcagatggtg
     4141 ccgctcgggc acgccgtacg gcggtccaag gcgccggccg ccgcgctcga cgcgatgttc
     4201 cgccagtccg gggtgatccg ggtcgagacc atccaccagc tcttcgacgt cgccgcgctg
     4261 ctggccaccc agccgctgcc taccggcgat cgggtggcga tcctcggcaa ctccgacgcg
     4321 ctggggctgc tcgccgccgg agccgccgag gcgtggggcc tgcacgtggt cggcgagccg
     4381 ctcgccctcg gcgccgaggc gaccgccgag gacttccgcc gggcgctggc ggccgtcttc
     4441 gccgacccgg ccgtcgactc cgtggtcgcc gtgttcatcc cgccgctgct caccctcgac
     4501 gaggacgtcg catcggtgct cgccgaggcg gccgcccggg ccaccaagac ggtcgtctcg
     4561 acgttcctcg ggatgcgcgg cgtgccggcc gccctgcggg ccggctccga cgggcgcggc
     4621 gcggtcccgt cctatcccac ccccgaggac gccgtccgcg ccctggtcgc ggccacccgc
     4681 tacgcgcagt ggcgtcgacg cccgatcggc tcggtgccgg ccctgcccga cgtcgacgtg
     4741 gctgctgccc gccagctggt cgagcaggtc ctcgccgagt gccccgacgg gcgggctctg
     4801 acccgcgacg agacggctgc gctgctgctg cccttcgggg tgaagatctg gcctgcggtg
     4861 ccggtgcacg acatcgactc ggcggtcgcc gcggccgagg ccctgggcta cccggtcgcg
     4921 ctcaaggcga cggcgcgcgc gctgcgccac cgcatcgacc tgggcggcat ccacctcggg
     4981 ctggccgatg aggccgacct gcgtcaggcc tacgcccagc tggtccggct gcaggccgac
     5041 gacgacctcg gcctggtcgt gcaaccgatg gccggcccgg ggctggccgt cgtggtgtcc
     5101 tcggccgagg acccgctgct cgggccctac ctggcatttg gcatcggcgg cgtcgtctcc
     5161 gagctgctcg gtgacctcgc ctaccggatc ccgccgctca ccgacgtgga cgccgccgag
     5221 atggtccgct cggtgcgtgc cgcgccgatg ctgttcggcc accgtggcgc cgagccggtc
     5281 gacgtcgcgg cggtcgagga cctgctgctg cgggtctccc ggctggccga cgagctgccc
     5341 gaggtcgcct cgctcgagct caacccggtg gtggtcggcg cccacgggct ggtcgtgctg
     5401 gcggccgagg cgtcggtggc ccaccccgcc gtgcgcaccg acagtccacg acgcagcctg
     5461 cccggctgag gagggcgcgc agccaccgcc gcgacggtgc caggatgggg gcatgcctga
     5521 gacccggagc accgagcgga cccgtccgtc gttgcaggac tcgatcgagc gcaccggcta
     5581 cttcccgcag gtcgtggccg acgcggtcga ggacgccctg gccgacgagc cggtgaccgc
     5641 acacctggtg caccaggaga cgaccttcga cagcgacgag gtgcgccggc acgtcacggt
     5701 cgtggccctg accgcgtctc ggctgctcgt ggcgcacacc gacgaccacc cggccggcgc
     5761 cgacacgccc accgcgctcg cgaccacctc ggtcgaggtg gtgccccttg acgctttgcg
     5821 ctcggtgatc gtcaaccggg tggtggccaa cccggcgacc tacgtgcgcg gcgccgaccc
     5881 gcaggaggtc gtgctgacgg tgggctgggg ggccgtcaac cggatcgacg tcgagccggc
     5941 cggctgctcg gaccccagct gcgaggccga ccacggctac accgggacct ccggggccga
     6001 cgacctctcg ctgcgggtca gctcggcggc cgagggaccc gacatcgtcg ccgaggcgct
     6061 ggcgctgggc cgggcgctgt cccgcgcgct ggctcgctcg ggcggtcagc gctgaccgtg
     6121 cgggggccgt cgggggaccc ggccgccgcc accgactgga gagcggccgc cgcgcgcctg
     6181 cgcccgccct acggcgaggc ctccctcgcc gacctgctgc cctcggtgtg tgcccacctc
     6241 ggcgtccccg gctgctccga cgtgctgggg ctgcctgcga ccgaccgggc gtgcgtgctg
     6301 ctcgtcgacg cgctcggctg gcacctgctg cgccgccacc ccgagaccgc gccgctgctg
     6361 cacgagctgg ccgaggaggg acggctgatc accgccggtt tcccgacgac cacgccgacc
     6421 agcctggcct gcctgggcac cgggctggct cccggcgggc acgggctcgt cggcttccag
     6481 gtgctggtgc ccggtccgga gcgggtgatg aacctgctca cgtgggacga gggggtggac
     6541 ccggtcgcct tccagccgag cgccaccctg atgcagcggg ccgaggcgag cggggtcacg
     6601 gtgaccaccg tcgggccacg cgcctaccgg ggcaccggac tcaccgtggc cgcgctgcgc
     6661 ggcggcgtcc accgcggtgg cgacagcatc ggcgagctgg tcgctggcat caccgccgcg
     6721 ctggcggagc cggggccggc cctggtctat gcctactacc ccggactcga ccagaccggg
     6781 caccgcaacg gctgcgactc tccggcctgg ctggccgagc tctcggtggt cgaccacatg
     6841 gtcgggctgc tgcgtgaggc gttgccctcc ggcgcggtcc tctatgtcac cggcgaccac
     6901 ggcatggtcg acgtgccgct ggaccggcgg atcgacatgg ccaccgagcc ggcgctgcgg
     6961 gagggggtgg cgctggtcgc cggcgagccg cgggtggtct acctgcgcac cgtgtgcggt
     7021 gccacgagtg acgtcatggc gacctggcgc gaccggctcg gttccgactt ctgggtgctg
     7081 ccgaccgagg aggtcatcga gctcgggtgg tacggcgccg tccgcgacgg ggtcgccgca
     7141 cggatcggag acatcgtggt cgccgcccgg gtgccggcgg tgtcgatcgt cgactcggag
     7201 cgcaccccgc gcgggctgct gcggctgaag gcgctgcacg gctcgctgac cgaccacgag
     7261 cggcacgtgc cgctgctgac cctgcgcggc tagcgcccca ccacgtgatc atgaaggctg
     7321 gccgccccta gcagggcggt acgccttcat gatcatccga gggtggctca gggctggcgg
     7381 cgggtgccca gcaggatgtc gtcccagctc ggcacgctgg ctcgtcgccc ggtccgccgg
     7441 cgcgcaccgg gcgcggaggt gggctcgggg ccggccggag cggcctgcgg gggctcggcc
     7501 gcctcggtgg actgggcctg ctcctcggcg gcctcggcgg cggcgccgcg ggctgcctcc
     7561 tcgcgcgcct cgctgaccga gatcaccggg gcgaggtggt cgccgggggc gcgccgcaga
     7621 cgcagcggct cgccgatccg gtcgagacct ccgtcggcct ccacgtcata gaccagct
//
LOCUS       DATIBB010000105         2849 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_1109316, whole genome shotgun sequence.
ACCESSION   DATIBB010000105 DATIBB010000000
VERSION     DATIBB010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2849)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2849)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2849
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1109316"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..517)
                     /locus_tag="VLC50_04035"
     CDS             complement(<1..517)
                     /locus_tag="VLC50_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HSF26669.1"
                     /translation="MTESTTGRNAEQLVNEQGGFPQQQEPPGLTGEMQPRPDHGEKSY
                     VGHGRLRGLRALITGGDSGIGRATAIAFAREGADVAISYLDEEQPDAEETAALVREAG
                     AKASLLPGDLTDRATCLAVVERAVAELGGLDVLVNNAAFQWARAESMTDISPERLDRV
                     FKTNLYAMFWLC"
     gene            675..1928
                     /locus_tag="VLC50_04040"
     CDS             675..1928
                     /locus_tag="VLC50_04040"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSF26670.1"
                     /translation="MRRVPPVTWTLLGAGAMTTLGTLPVFLLSSQSVFVRRELDLDDG
                     QFGVAVSAFFAAAATFALLGGGLVDRLGRRRGTVVAGLVSAAGCAAVGLGVSGYASLV
                     PAMVVLGVGNAACQVTSNLTVARAVPPHRRGLGFGVKQSAIPLSIVIAGLAVPTVGAW
                     VGWRGTFLLTGAAALAVVVRGLLLPAAVASGGAASGPADRPPLRALVTVMLGIALASA
                     AANSLGSFTAAWGFEVGLSPGQAGLLMAVGSALNIAVRVLAGHRADLRFGRNLPVVAA
                     QMVVGAVALAVLSVPSPVTVVAAGLVAFAIGWSWPGLLLYAVVRVGRDAPAAATGIVQ
                     AGAFAGGAAGPALFGLVVVASSYETAWRAAAVLFLLAAGLVTLARRMFIADLVARPPA
                     QSFGYGGGRTAPLRTTDVRPEQEDR"
     gene            1987..2772
                     /locus_tag="VLC50_04045"
     CDS             1987..2772
                     /locus_tag="VLC50_04045"
                     /inference="COORDINATES: protein motif:HMM:NF016214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HSF26671.1"
                     /translation="MHFPLARKRCRALLGHSHAMSGLAVGAATLPVVPVQTPLTQLTW
                     VVAVGGFAMLPDLDTGGVTSRRGFPRMHGSTIALMWGPLTCVFAGLVARAAGGHRKGT
                     HSLLGLFLWALAATALSSFYAGRLVLVALGIGLALEALAFAIPGRFEEMWPVNLAASF
                     GAAWWLLGEPGPSGMGENYPLWLPSAVLVGCVTHVVGDLLTVGGVPLAWPLSDARLSV
                     GLFQTGSPWERLLVAPALLAVAVWMVVTRTYADAGPVLNVIGI"
BASE COUNT          283 a         1014 c         1089 g          463 t
ORIGIN      
        1 ggcagagcca gaacatcgcg tagaggttgg tcttgaacac ccggtcgagg cgttcggggg
       61 agatgtcggt catcgactcg gcccgcgccc actggaacgc ggcgttgttg acgagcacgt
      121 ccagacctcc gagctcggcg accgcgcgtt cgacgaccgc gaggcaggtg gcccggtcgg
      181 tgaggtcgcc ggggagcagg ctcgccttgg cgccggcctc ccgcacgagc gcggcagtct
      241 cctccgcgtc cggctgctcc tcgtcgaggt aggagatcgc gacgtcggcg ccctcccggg
      301 cgaaggcgat cgcggtcgcc cggccgatgc cggagtcgcc gccggtgatc agcgcccgca
      361 gtccccgcag ccggccgtgg ccgacgtagc tcttctcgcc gtggtcgggc cggggctgca
      421 tctcgccggt caggccggga ggctcctgct gctgggggaa gccgccctgc tcgttgacga
      481 gctgctcggc gttcctgcct gtcgtcgact ccgtcatccg acctccttcg acgtggttac
      541 gccgacgctg tgcccagggt ccccgccgac aaacgcgagg acaagcccgc ggacgagccc
      601 ggccgctggt gcggggacga gcccggcgcc gacccttggc gactgcccgc tgacgggtcg
      661 ggtaaggatg aggcatgcgc cgagtcccac cggtcacgtg gacgctgctg ggcgccggcg
      721 cgatgaccac cctgggcacg ctgccggtgt tcctgctcag ctcccagtcg gtgttcgtgc
      781 gccgcgagct cgatctcgac gacggacagt tcggcgtcgc ggtgagcgcc ttcttcgccg
      841 cggcagcgac gttcgcactg ctcggcggcg ggctcgtcga ccggctcggg agacgtcgcg
      901 gcaccgtggt ggccggcctg gtctccgccg ccgggtgtgc tgccgtcggg ctcggggtca
      961 gcggctacgc ctcgctggtg ccggccatgg tcgtgctcgg tgtcgggaac gccgcctgcc
     1021 aggtcacgtc gaacctgacc gtggcccgcg cggtgccgcc gcaccgccgg gggctggggt
     1081 tcggcgtgaa gcagtcggcg atcccgctct cgatcgtgat cgccgggctc gcggtgccca
     1141 cggtgggcgc ctgggtcggc tggcgcggga ccttcctgct gaccggggcc gcggcactcg
     1201 ccgtggtggt gcgcggcctg ctgctgcccg ccgcggtcgc ctccggcggc gcggcctccg
     1261 ggccggccga ccggcccccg ctgcgtgccc tggtgaccgt gatgctcggc atcgccctgg
     1321 ccagcgcggc cgccaactcc ctcggctcgt tcaccgccgc ctgggggttc gaggtcgggc
     1381 tctcaccggg ccaggcgggc ctgctgatgg ccgtcgggag cgcgctgaac atcgcggtcc
     1441 gtgtcctcgc cgggcaccgt gccgacctgc ggttcggcag gaacctgccg gtggttgcgg
     1501 cgcagatggt ggtcggggcg gtggcactcg cggtgctctc ggtcccctca ccggtgaccg
     1561 tggtcgcggc cgggctggtc gccttcgcga tcggctggtc ctggccgggg ctgctgctct
     1621 acgccgtggt ccgggtcgga cgggacgccc ccgcagcggc caccggcatc gtccaggccg
     1681 gagccttcgc cgggggcgcc gcaggcccgg ccctgttcgg cctggtcgtg gtcgccagca
     1741 gctacgagac cgcgtggcgg gcggcggccg tgctgttcct gctcgccgcg gggctggtga
     1801 cgctggcccg gaggatgttc atcgccgacc tggtggcgag gccgccggcg cagtcgttcg
     1861 ggtacggcgg gggccggacc gcgcccctgc gcaccaccga cgtgcgaccg gagcaggagg
     1921 accggtagcg gtccgggcgc ctccggcgtg agacgtcggc gggggtcatt acgatgacgc
     1981 gtccgagtgc actttcccct tgccagaaag cgatgtcgcg cgttgttggg ccactcccat
     2041 gccatgtccg ggcttgccgt cggcgcggcc accctgcccg tcgtacccgt ccagacgccg
     2101 ttgacccagc tcacctgggt ggtcgcggtc ggcgggttcg cgatgctgcc cgacctcgac
     2161 acgggaggcg tcacctcgcg gcgcgggttc ccgcggatgc acggctccac gatcgccctg
     2221 atgtgggggc cgctgacctg cgtgttcgcg gggctggtgg cccgggcggc cggcgggcac
     2281 cgcaagggca cccactccct gctcggcctt tttctgtggg cgctcgcggc gactgccctg
     2341 tcgtccttct acgccggacg gctggtgctg gtggcgctcg gcatcggcct ggccctcgag
     2401 gcactcgcct tcgccatccc gggcaggttc gaggagatgt ggccggtgaa cctcgcggcg
     2461 tcgttcggcg ccgcctggtg gctgctcggc gagccggggc cctccgggat gggcgagaac
     2521 tacccgctgt ggctgcccag cgcggtgctg gtcggttgtg tcacgcacgt ggtcggcgac
     2581 ctgctcaccg tcgggggcgt cccgctggcg tggccgctct cggacgcgcg gctcagcgtc
     2641 ggcctgttcc agaccgggtc gccctgggaa cgcctcttgg tggcgccggc gctgctggcc
     2701 gtggcggtgt ggatggtggt gacccgcacc tacgccgacg ccggtccggt gctcaacgtg
     2761 atcgggatct agacaggggg tttgggatcc gggcaggcgg gggtacccct ggtgtcctgg
     2821 cgcccacgca acacccgagg aggacccat
//
LOCUS       DATIBB010000106         3977 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_1767718, whole genome shotgun sequence.
ACCESSION   DATIBB010000106 DATIBB010000000
VERSION     DATIBB010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3977)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3977)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3977
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1767718"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..298
                     /locus_tag="VLC50_04050"
     CDS             <1..298
                     /locus_tag="VLC50_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012015415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HSF26672.1"
                     /translation="AVVEPSWTDFHVAYRVGSRHRLDRGAAGRAILAARTGGETVVVS
                     DGELQAGAQGVAAALTGLPVIEASVGVVALSALDVATVGPRVRAAADAVAVALR"
     gene            complement(323..790)
                     /locus_tag="VLC50_04055"
     CDS             complement(323..790)
                     /locus_tag="VLC50_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013288920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HSF26673.1"
                     /translation="MPIDELDAQIIRLFAQEPRVGVLEASRRLKVARGTVQARLDRMT
                     RAGIVTGFGPDVDPAAIGYGVTAFATLEIRQGHGHEAIAAHLASIPEVLEAYTITGAA
                     DMYCRIVARSNADLQRVIDMLVAFGGIERTSTVVALATQVPFRVLPLVDEAAR"
     gene            930..2159
                     /gene="hppD"
                     /locus_tag="VLC50_04060"
     CDS             930..2159
                     /gene="hppD"
                     /locus_tag="VLC50_04060"
                     /EC_number="1.13.11.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003868 - 4-hydroxyphenylpyruvate
                     dioxygenase activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HSF26674.1"
                     /translation="MTTATPRTAAVPSPLTPEEREANLDLDALKTLVGLVDYDESKDP
                     FPVTAVDAAVFIVGNARQTAHFYQLAFGMELVAYAGPETGQRDHASYVLRSGSARFVI
                     TGGVHPSSPLHEHHRRHGDGLSDIALEVPDVDRCIAHARAMGARILEEPHDVADQHGS
                     VRMAAIATYGDTRHTLLDRSRYSGPYLPGYVARTTAVTRPAEHPKRLFQAIDHIVGNV
                     ELGKMDEWVGFYNTVMGFVNMAEFIGDDIATDYSALMSKVVANGNHRVKFPLNEPAIA
                     KHKSQIDEFLEFYDGAGAQHVALATNDLLRTVDMLRANGVQFLETPDSYYDDPELRAR
                     IGQVRVPIEELKARRILVDRDEDGYLLQIFTNPIGDRPTVFYEFIERHGSLGFGKGNF
                     KALFEAIEREQERRGNL"
     gene            complement(2421..3662)
                     /gene="fahA"
                     /locus_tag="VLC50_04065"
     CDS             complement(2421..3662)
                     /gene="fahA"
                     /locus_tag="VLC50_04065"
                     /EC_number="3.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018500760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004334 - fumarylacetoacetase activity
                     [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetase"
                     /protein_id="HSF26675.1"
                     /translation="MTSTPTSPSTSWVPVPDGSPFPVAHLPYGVFSVADEAPRVGVAI
                     GAHVLDLAAAAAAQGLDGGHVFAEPTLNAFLALGRPAWTAVRSWVTEVLTHRAHEDLM
                     SSFLVPLDQVRMHLPFEVADYVDFYSSVDHATNVGHIFRPDSAPLPPNWKHLPIGYHG
                     RSGTVVVSGTPVRRPCGQRKTTRDAPPSYGPCLRLDIEAEVGFVVGTPSERGRAVPAS
                     DFREHVFGLVLLNDWSARDIQAWEYVPLGPFLGKSFATSISAWVTPLDALAAARVTPP
                     VQDPAPLPYLHDEAGWGLDLALQVDWNGTTISRPPFARMYWTPAQQLAHLTVNGASLR
                     TGDLYASGTVSGPDKDQRGSLLELTWNGAEPVRLADGSERRFLEDGDTVTISAWAPGA
                     DGARIGLGEVTGTILPATPTQ"
     gene            complement(3659..>3977)
                     /locus_tag="VLC50_04070"
     CDS             complement(3659..>3977)
                     /locus_tag="VLC50_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013131363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF26676.1"
                     /translation="ETPYKCTAGLHHAVRHTDPVTGFEHHGFLNLLLATHAATQGADL
                     AAVTALLARRDGAALARTAAGLDPTEALVARSFFVAFGSCSIAEPADDLLDLDLLTTE
                     LPA"
BASE COUNT          588 a         1401 c         1444 g          544 t
ORIGIN      
        1 cgcggtggtc gagccgtcgt ggaccgactt ccacgtcgcc taccgggtcg gttcgcgcca
       61 ccggctcgac cggggggccg ccgggcgggc gatcctggcc gcgcgcaccg gcggcgagac
      121 cgtcgtggtc tccgacggcg agctgcaggc cggcgcgcag ggggtggccg ccgcgctgac
      181 cggcctgccg gtgatcgagg cctcggtcgg ggtggtggcg ctcagtgcgc tcgatgtcgc
      241 caccgtgggc ccgcgggtgc gggccgccgc cgacgcggtg gcggtggccc tgcgctgacg
      301 ctcgcggctg acgctcgcgc cgtcagcggg cggcctcgtc gaccaggggc agcacccgga
      361 acggcacctg ggtggccagc gccaccaccg tcgaggtccg ctcgatgccg ccgaacgcga
      421 ccagcatgtc gatgacccgc tgcaggtcgg cgttggagcg ggccacgatc cggcagtaca
      481 tgtcggctgc gccggtgatg gtgtaggcct cgagcacctc ggggatcgac gccaggtgcg
      541 cggcgatcgc ctcgtgcccg tggccctggc ggatctccag ggtggcgaac gccgtcacgc
      601 cgtagccgat cgcggccggg tccacgtcgg ggccgaaccc ggtgacgatg ccggcccggg
      661 tcatgcggtc caggcgggcc tgcacggtgc cgcgggcgac cttgagtcgt cgcgaggcct
      721 ccagcacgcc cacccgcggc tcctgggcga acagcctgat gatctgggcg tccagctcgt
      781 cgatcggcat gccgcctccg tcctcgtggg gtggtcctgg ctggtcacat tgtccatggt
      841 cacggcatct cttgggtcca acgtgcgcat catgaccact ggcggctccc caggttgccc
      901 aaccaccaac cggcgatcac agtgcgggca tgaccactgc cacgccgcgc accgccgccg
      961 taccgtcgcc gctgaccccc gaggagcgcg aggccaacct cgacctcgac gccctcaaga
     1021 cgctggtcgg gctggtggac tacgacgaga gcaaggaccc gttcccggtc accgccgtgg
     1081 acgccgcggt gttcatcgtc ggcaacgcgc gccagaccgc gcacttctac cagctcgcgt
     1141 tcggcatgga gctggtcgcc tacgccggcc ccgagaccgg ccagcgcgac cacgcgtcct
     1201 atgtgctgcg ctcgggctcg gctcgcttcg tcatcaccgg cggggtgcac ccgtccagcc
     1261 cgctgcacga gcaccaccgt cgccacggcg acgggctgtc cgacatcgcg ctagaggtac
     1321 ccgacgtcga ccggtgcatc gcccacgccc gggccatggg cgcccgcatc ctcgaggagc
     1381 cgcacgatgt cgccgaccag cacggcagcg tgcggatggc agcgatcgcg acgtacggcg
     1441 acacccgcca caccctgctc gaccgctccc gctactccgg gccctacctg cccggctacg
     1501 tcgcccggac caccgcggtg acccgcccgg ccgagcaccc caagcggctg ttccaggcca
     1561 tcgaccacat cgtcggcaac gtcgagctcg gcaagatgga cgagtgggtg ggcttctaca
     1621 acacggtcat gggcttcgtg aacatggccg agttcatcgg tgacgacatc gccaccgact
     1681 actccgcgct gatgtccaag gtggtggcca acggcaacca tcgggtcaag ttcccgctca
     1741 acgagccggc gatcgccaag cacaagtcgc agatcgacga gttcctcgag ttctacgacg
     1801 gggcaggcgc ccagcacgtc gcgctggcca ccaacgacct gctgcgcacg gtcgacatgc
     1861 tgcgggccaa cggcgtgcag ttcctcgaga cgcccgactc ctactacgac gaccccgagt
     1921 tgcgcgcacg gatcggccag gtgcgggtcc cgatcgagga gctgaaggcc cggcgcatcc
     1981 tcgtcgaccg cgacgaggac ggctacctcc tgcagatctt caccaacccg atcggcgacc
     2041 gcccgacggt cttctacgag ttcatcgaac ggcacgggtc gctgggcttc ggcaagggca
     2101 acttcaaggc gctgttcgag gcgatcgagc gcgagcagga gcgccgcggc aacctctgac
     2161 cctgagcccc cgcggtgatc aagcaagccg tgcccacacc tgggcggtga tcaagcaatc
     2221 cgtgcccaca cctgcgtggt gatcaagcaa gtcttgtacg gcgccggcag ggcgcagcgc
     2281 cgtacgacgt agtcatacgc ggcattcatc gatagtcgac agatcacccg gctgcagccg
     2341 tacacggatc gcttgatcac ggtcagtcgg cgagcaccga ttgcttgatc acgaacacca
     2401 ggcgggcacg ggttgcttga tcactgagtg ggggtggcgg gcaggatggt gccggtcacc
     2461 tcgccgagcc cgatgcgtgc cccgtcggcg ccgggcgccc aggccgagat cgtcaccgtg
     2521 tcgccgtcct cgaggaaccg gcgctccgac ccgtcggcca gcctgaccgg ctcggcgccg
     2581 ttccaggtca gctcgagcag cgagccgcgc tggtccttgt ccggtccgga gaccgtgccg
     2641 gaggcgtaga ggtcgccggt gcgcagcgag gcgccgttga cggtgaggtg ggccagctgc
     2701 tgggccgggg tccagtacat ccgggcgaac ggcgggcgcg agatcgtcgt gccgttccag
     2761 tccacctgca gcgccaggtc gagcccccag cccgcctcgt cgtgcaggta gggcagcggg
     2821 gccgggtcct gcaccggcgg cgtgacccgg gcggcggcga gcgcgtcgag tggggtcacc
     2881 caggccgaga tcgacgtcgc gaacgacttg ccgaggaacg gcccgagcgg cacgtactcc
     2941 caggcctgga tgtcgcgcgc gctccagtcg ttgagcagca ccagcccgaa gacgtgctcg
     3001 cgaaagtcag aggcgggtac ggcgcggccg cgctcggacg gcgtgcccac gacgaaaccc
     3061 acctcggcct cgatgtccag gcgcaggcag ggcccgtagg acggcggcgc gtcgcgggtc
     3121 gtcttgcgct gcccgcacgg gcgtcgtacc ggcgtgccgg agaccacgac cgtgccggag
     3181 cggccgtggt agccgatcgg caggtgcttc cagttgggcg gcagcggcgc cgagtcgggc
     3241 cggaagatgt gcccgacgtt ggtcgcgtgg tcgaccgagg agtagaagtc gacgtagtcg
     3301 gccacctcga acggcaggtg catgcgcacc tggtcgagcg ggacgaggaa ggacgacatg
     3361 aggtcctcgt gggcccggtg ggtgagcacc tcggtgaccc aggaccgcac cgcggtccag
     3421 gccgggcggc ccagcgccag gaacgcgttg agcgtcggct cggcgaacac gtgcccgccg
     3481 tccaggccct gggcggcggc ggccgcggcc aggtcgagca cgtgcgcgcc gatcgccacg
     3541 ccgacccgcg gggcctcgtc ggcgacggag aacacgccgt agggcaggtg cgccaccggg
     3601 aacggtgagc cgtcgggtac cgggacccag ctggtcgacg ggctggtcgg cgtggaggtc
     3661 atgcgggcag ctccgtggtc agcaggtcga ggtcgagcag gtcgtcggct ggttcggcga
     3721 tgctgcacga gccgaaggcg acgaagaacg agcgggccac cagcgcctcg gtcgggtcga
     3781 gcccggccgc ggtccgggcc agcgcggcac cgtcgcgccg ggccagcagc gcggtcaccg
     3841 cggcgaggtc ggcgccctgg gtggcggcgt gggtggccag cagcaggttg aggaacccgt
     3901 ggtgctcgaa cccggtcacc gggtcggtgt gccgcacggc gtggtgaagg ccggcggtgc
     3961 acttgtacgg cgtctcc
//
LOCUS       DATIBB010000107         2225 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_1503873, whole genome shotgun sequence.
ACCESSION   DATIBB010000107 DATIBB010000000
VERSION     DATIBB010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2225)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2225)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2225
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1503873"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..756)
                     /locus_tag="VLC50_04075"
     CDS             complement(<1..756)
                     /locus_tag="VLC50_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005482695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HSF26677.1"
                     /translation="MDVLVVGSGAREHALVRALRADPQVDRLWCAPGNAGIAADVPTE
                     PLDLADPAAITALAQRLRAELVVIGPEGPLVAGAADAVRAAGIACFGPSGAAARLEGS
                     KAFAKDVMAAAGVPTASARLCESADQVDAALDEFGPPYVVKDDGLAAGKGVVVTTDRA
                     AARAHAERCGRVVIEEYLDGPEVSLFAITDGTTVLPLVPAQDFKWVGDGDTGPNTGGM
                     GAYSPLPWAPAGLVDEVRDRVLAPTVAEMARRGT"
     gene            complement(845..2209)
                     /locus_tag="VLC50_04080"
     CDS             complement(845..2209)
                     /locus_tag="VLC50_04080"
                     /inference="COORDINATES: protein motif:HMM:NF004718.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HSF26678.1"
                     /translation="MTASTTSTDSARAERVRALDDSFVFHPWAAQGPRGALAIERGEG
                     SYVWDVQGNRYLDFTGQLVFTNIGFSHPKVVAAIQEQAARLATLSPGYATEVRGEAAR
                     MIVELAGPSYSKVFFTNAGTEAVEHAVRMARQHTGRPKLLSAYRSYHGATSTSIALTG
                     EPRRWANDAGTAGVAHFFGPYLYRSAFHATTEAEECQRALEHLEQVVALEGPSTIAAI
                     VLESVVGSAGLLPPPAGYLRGVREICDRHGIVFIADEVMAGFGRTGRWFAFQHDDVVP
                     DLITFAKGVNSGYVPLGGVIVAEPIVETFRSRPYPGGLTYSGHPLACAAAVATMTAMA
                     EEGIVENAARIGADVLGPGLRGLAEKHPSVGEVRGRGVFWGVELVRDRASREPLVPYA
                     ARGAAAAPMAAIAKACRERGVLAMVAGNRIHVVPPCNVTDAEVREGLAAIDAALEGAD
                     ALTA"
BASE COUNT          287 a          821 c          851 g          266 t
ORIGIN      
        1 ggtgccgcgg cgggccatct cggccacggt cggggcgagc acccggtcgc gcacctcgtc
       61 gacgaggccg gccggggccc agggcagcgg cgagtaggcg cccatgccgc cggtgttggg
      121 cccggtgtcg ccgtcgccga cccacttgaa gtcctgcgcc ggcaccagcg gcagcaccgt
      181 ggtgccgtcg gtgatggcga acagcgacac ctcgggcccg tcgaggtact cctcgatgac
      241 gacccggccg cagcgctcgg cgtgggcccg ggccgccgca cgatcggtgg tcacgacgac
      301 gcccttgccg gcggccagcc cgtcgtcctt gacgacgtac ggcggtccga actcgtcgag
      361 cgccgcgtcc acctggtcgg cgctctcgca gagccgcgcg gacgcggtcg ggacgccggc
      421 ggcggccatg acgtccttgg cgaacgcctt cgagccctcg agccgggccg ccgcgccgct
      481 gggtccgaag caggcgatgc cggcggcgcg cacggcgtcg gcggccccgg ccaccagcgg
      541 cccctcgggg ccgatgacga cgagctcggc acggagtcgc tgggccaggg cggtgatggc
      601 agcggggtcg gcgaggtcga gcggctcggt cggtacgtcg gcggcgatgc cggcgttgcc
      661 cggggcgcac cagagccggt cgacctgcgg gtcggcgcgg agggctcgca ccagcgcgtg
      721 ctcgcgggcg ccggacccga cgacgaggac gtccacgggg ccggagccta tcgaccggtg
      781 cgggcccgcc gcgcagccgc cgtacgcccc aagacccccg ccggtgatca gccggtcagg
      841 gtgctcaggc ggtcagggcg tcggcgccct ccagggcggc gtcgatggcg gccaggccct
      901 cgcggacctc ggcgtcggtg acgttgcacg ggggtacgac gtggatccgg ttgccggcca
      961 ccatcgcgag caccccgcgc tcccggcaag ccttcgcgat cgcggccatc ggcgccgccg
     1021 cggcaccgcg ggcggcgtag ggcaccagcg gctcgcggct ggcccggtcg cggaccagct
     1081 cgacccccca gaacaccccg cgcccgcgca cctcgccgac cgacgggtgc ttctcggcca
     1141 gcccgcgcag ccccggcccg agcacgtcgg cgccgatccg ggcggcgttc tccacgatgc
     1201 cctcctcggc catcgcggtc atcgtcgcga ccgccgccgc gcaggccagc gggtgcccgg
     1261 agtaggtcag cccgcccggg tagggccggg accggaacgt ctcgacgatc ggctcggcca
     1321 cgatcacccc gccgagcggg acgtagccgg agttgacgcc cttggcgaac gtgatgaggt
     1381 cgggcaccac gtcgtcgtgc tggaaggcga accagcgccc ggtccggccg aacccggcca
     1441 tcacctcgtc ggcgatgaag acgatgccgt gccggtcgca gatctcccgg accccgcgca
     1501 ggtaaccggc cggcggcggc agtaggccgg cactgccgac gaccgactcg agcacgatcg
     1561 ccgcgatcgt ggacgggccc tccagcgcga ccacctgctc gaggtgctcg agcgcgcgct
     1621 ggcactcctc ggcctcggtg gtggcgtgga aggccgagcg gtagaggtag ggcccgaaga
     1681 agtgcgccac cccggcggta cccgcgtcgt tggcccaccg ccggggctcg ccggtcagcg
     1741 cgatcgaggt ggaggtcgcg ccgtggtagc tgcggtaggc cgagagcagc ttgggccgac
     1801 cggtgtgctg acgggccatg cggacggcgt gctcgaccgc ctcggtgccg gcgttggtga
     1861 agaacacctt gctgtacgac ggcccggcca gctcgacgat catgcgggcg gcctcgccgc
     1921 ggacctcggt ggcgtacccc ggggagaggg tggccagccg ggcggcctgc tcctggatcg
     1981 cggcgaccac cttggggtgc gagaacccga tgttggtgaa caccagctgc ccggtgaagt
     2041 cgaggtagcg gttgccctgc acgtcccaga cgtaggagcc ctcaccgcgc tcgatggcca
     2101 gcgcgccgcg cgggccctgc gccgcccacg ggtggaacac gaaggagtcg tcgagcgcgc
     2161 gcacccgctc ggcccgggcg ctgtcggtgg acgtcgtcga agcggtcatg ccgggaatgt
     2221 aaccc
//
LOCUS       DATIBB010000108         4815 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_1504389, whole genome shotgun sequence.
ACCESSION   DATIBB010000108 DATIBB010000000
VERSION     DATIBB010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 4815)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4815)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4815
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1504389"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(190..675)
                     /locus_tag="VLC50_04085"
     CDS             complement(190..675)
                     /locus_tag="VLC50_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012785767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HSF26679.1"
                     /translation="MRSTAPRPHLGPDGHLDDVSKRIIEQLQADGRRSYAAIGKAVGL
                     SEAAVRQRVQRLLEAGVMQIVAVTDPLQLGFPRQALIGIEVDGEVEPVADKLAAMPEV
                     DYVVITAGSFDILAEVVCENDDQLLDVISSQIRRLGGVRRTETFVYLQLRKQTYSWGA
                     V"
     gene            890..2389
                     /locus_tag="VLC50_04090"
     CDS             890..2389
                     /locus_tag="VLC50_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018851008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-aminobutyraldehyde dehydrogenase"
                     /protein_id="HSF26680.1"
                     /translation="MAAQGHQHVGGRRLTGSSSARAAVLDPATGDRVVDLDLAAPADV
                     DRAVQAARAAFPDWSGATPAVRAQALTRLAARLDELAEELASVETRQTGKPIRLSREF
                     DVPGTIDNTAFFAGAARHLEGRATGEYSPDHTSSVRREPIGVVGSIAPWNYPLQMAGW
                     KILPAVAAGNTIVLKPSELTPLTSLMLAEAAREAGLPDGVVNVVTGTGAEAGEALVSH
                     DDVDMVSFTGSSRVGRRVMALAAEGARGPRRVHLELGGKAPFVVFDDADLSAAVHGAV
                     AGALINCGQDCTAATRAYVARPLYDAFVEGVADLMAGVRLGDPTDPTTDLGPLVSRTQ
                     QRRVAGFVDRAVAAGAKVVRGGSIPGGALTRGAYYEPTLVVDASPDSEIVRDEVFGPV
                     LVVLPFDSDDQALAMANDSPYGLAASAWTRDTFRALRATREIAAGCVWINDHIPIVSE
                     MPHGGYRASGFGKDMSAYSLEDYTNVKHVMQERTGIATKAWHRTVFTQT"
     gene            2436..3653
                     /locus_tag="VLC50_04095"
     CDS             2436..3653
                     /locus_tag="VLC50_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HSF26681.1"
                     /translation="MDPKLTSSLPFARGLLTRRTLLTGVGAGAAALALAACGTEGTAT
                     NPTSAATGTGAAPGQDFSDTEKQIKWSNWPEYIDVDDNTQARPTIEEFQKQTGIKVTY
                     TEDYNDNDEFFAKVRPQLSAGQDTGRDVWCSTDWMVARLIRLGWVQKLDKANIPNAAN
                     LEPDLQNVEFDKGRVYSLPWQSGFAGIGYNPAATGGKKIESVDQLLTDASLKGKVTLL
                     TEMRDTVGLVMLAQGKDPADFTDADFDAAIAEIQRAKDAGQLRGFTGNEYGKPLAAGD
                     IAACFAWTGDVVQLQADNPDLGYALPATGHTLWSDNFVIPNKARHKANAEKLINYYYG
                     PEVMAQVVAYVNYISPVAGVRDIVVADDPETADNPLIFPSADVLAKSHVFRGLTEEEE
                     TKYNKAFQALIGG"
     gene            3657..>4815
                     /locus_tag="VLC50_04100"
     CDS             3657..>4815
                     /locus_tag="VLC50_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020046186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSF26682.1"
                     /translation="MTTAAAPGQLGDVTDGANGSDLRLVGLTKRFGDFTAVETLDLTI
                     PAGSFFALLGPSGCGKTTTLRMVAGLEEPTQGQILIGDLDITDTKPYRRPVNTVFQNY
                     ALFPHLDIYENVAFGLRRRGRKDIDAKVGEALELVQLGSYARRKPAQLSGGQQQRVAL
                     ARAIVNRPAVLLLDEPLGALDLKLRRQMQIELKQIQTEVGITFVHVTHDQEEAMTMAD
                     TIAVMDGGRIEQLGDPTTLYELPATTFAANFLGQSNLLAATVVGRDGDDLVVDVHGAR
                     MALAASRTPVTNGAMWLGIRPEKLRVLTADEAVDGRNSLSGTVTDASFIGVSTHYLVR
                     LPWGQEVTVVQQNDGTPPLRPGERVSVAWERTHGFGLDASQDAHAGEQYVVG"
BASE COUNT          771 a         1730 c         1639 g          675 t
ORIGIN      
        1 aagcgcggcg gctgggaggg atcgccgtag gcgacgctct cggtggtcat ggtggcgtcc
       61 ttcgtcgtag ggctcagcgc tgttatcgca caccagactg aggcttggca agcgattcag
      121 cgcgtcatgc cggtcagtac gacgaaatca gttcctcacc gctcgcgtta cgacgagatc
      181 cgcactcggc tacaccgcgc cccacgagta ggtctgcttg cgcagctgca ggtagacgaa
      241 cgtctcggtg cggcgcacgc caccgaggcg gcggatctgc gaggagatca cgtcgagcag
      301 ctggtcgtcg ttctcgcaga ccacctcggc gaggatgtcg aaggagcccg cggtgatcac
      361 cacgtagtcg acctcgggca tcgcggccag cttgtcggcc accggctcga cctcgccgtc
      421 gacctcgatg ccgatcagcg cctggcgcgg gaaccccagc tgcagcgggt cggtgaccgc
      481 gacgatctgc atcacgccgg cctcgagcag ccgctgcacc cgctggcgca ccgcggcctc
      541 cgagagcccg accgccttgc cgatcgcggc gtaggagcgc cggccgtcgg cctgcagctg
      601 ctcgatgatc cgcttggaga cgtcgtcgag gtgcccgtcg gggccgaggt gcgggcgcgg
      661 tgcggtggat ctcacggcgg catcctcccg gcgatcaagg cgcggcggca agccgggcgg
      721 gctgccgtgg caccggtcgg gcatcgaacc tgataggtca ccgaatgata gcgatttcgt
      781 cgtcccgtgg acgaggcgca acggaatcgc ttgtgtgggg ccgtcgcgca tgtcaggatc
      841 ggccgcgcgg tcagcaccgg tcggaagcac tcggatcagg gggtcggctg tggcagcgca
      901 gggtcaccag cacgtcggcg gacgccgtct cacgggctcg tcgtcggcgc gggccgcggt
      961 gctcgaccca gccaccggag accgtgtcgt cgacctcgat ctggccgcgc cggccgacgt
     1021 cgaccgggcg gtgcaggccg cgcgcgccgc gttccccgac tggtcgggcg ccacaccggc
     1081 ggtgcgcgcg caggcgctga cccggctcgc ggcgcgcctc gacgagctgg ccgaggagct
     1141 ggccagcgtg gagacccgcc agaccggcaa gccgatccgg ctctcccgcg agttcgacgt
     1201 gcccggcacg atcgacaaca ccgcgttctt cgccggggcg gcccgccacc tcgagggccg
     1261 ggccaccggc gagtactcgc ccgaccacac gtcctccgtg cgtcgcgagc cgatcggcgt
     1321 cgtcggctcg atcgccccgt ggaactaccc gctgcagatg gcaggctgga agatcctgcc
     1381 ggcggtcgcg gccggcaaca cgatcgtgct caagcccagc gagctcacgc cgctgaccag
     1441 cctgatgctg gccgaggcgg cgcgcgaggc cggcctgccc gacggcgtgg tcaacgtcgt
     1501 gaccggcacg ggcgcggagg ccggtgaggc gctggtcagc catgacgacg tcgacatggt
     1561 ctcgttcacc ggctcgagcc gcgtcggtcg tcgggtcatg gcgctggccg ccgagggtgc
     1621 gcgcggtccg cggcgggtgc acctcgagct ggggggcaag gcgccgttcg tcgtcttcga
     1681 cgacgccgac ctgtcggccg ctgtccatgg tgccgtcgcc ggggcgttga tcaactgtgg
     1741 ccaggactgt acggcggcca ctcgggccta cgtcgcgcgc ccgctctacg acgcgttcgt
     1801 cgagggggtg gccgacctca tggccggcgt acggctgggt gaccccaccg acccgaccac
     1861 cgacctcggc ccgctcgtgt cgcggaccca gcagcggcgg gtggccggct tcgtcgaccg
     1921 ggccgtggcc gcgggcgcca aggtcgtgcg cggcgggtcg atccccggcg gggccctgac
     1981 ccgtggggcc tactacgagc cgaccctcgt cgtcgacgca agcccggact ccgagatcgt
     2041 gcgggacgag gtcttcgggc cggtcctcgt ggtcttgccc ttcgactccg acgaccaggc
     2101 cctggcgatg gccaacgact cgccctacgg gctggccgcc tcggcctgga cgcgcgacac
     2161 cttccgggcg ctgcgggcca cccgcgagat cgcggccgga tgcgtctgga tcaacgacca
     2221 catcccgatc gtcagcgaga tgccgcacgg cggctaccga gcgtccggct tcggcaagga
     2281 catgtcggcc tactcgctcg aggactacac caacgtcaag catgtgatgc aggagcgcac
     2341 cggcatcgcg accaaggcct ggcaccgcac ggtcttcacc caaacctgag acagcacccg
     2401 gtcgtccgga gagacaattc ccacggagga acctgatgga tcccaagctc acatcctcat
     2461 tgccgtttgc gcgcgggctc ctgacgcgcc gcacgctcct caccggcgtc ggggcgggcg
     2521 ccgccgcgct cgccctcgcc gcctgcggga ccgaggggac cgcgaccaac ccgaccagcg
     2581 ccgcgaccgg caccggcgcc gcaccgggcc aggacttctc cgacaccgag aagcagatca
     2641 agtggtccaa ctggccggag tacatcgacg tcgacgacaa cacccaggcc cggcccacga
     2701 tcgaggagtt ccagaagcag accgggatca aggtcaccta caccgaggac tacaacgaca
     2761 acgacgagtt cttcgcgaag gtccgcccgc agctgtccgc ggggcaggac accggccgtg
     2821 acgtctggtg cagcaccgac tggatggtcg cccggctgat ccggctcggc tgggtgcaaa
     2881 agctggacaa ggccaacatt cccaacgccg ccaacctcga gcccgacctg caaaacgtcg
     2941 agttcgacaa gggccgggtc tactcgctgc cctggcagag tgggtttgcc ggtatcggct
     3001 acaaccccgc ggccaccggc ggcaagaaga tcgagtcggt cgaccagctg ctcaccgacg
     3061 caagcctcaa gggcaaggtc accctgctca ccgagatgcg cgacaccgtc gggctagtca
     3121 tgctcgccca gggcaaggac ccggccgact tcaccgacgc cgacttcgac gccgccatcg
     3181 ccgagatcca acgggccaag gacgccggcc agctgcgcgg gttcaccggc aatgaatacg
     3241 gcaagccgct ggctgccgga gacatcgccg cctgcttcgc ctggaccggc gacgtggtcc
     3301 agctgcaggc cgacaacccc gacctcggct acgccctgcc ggccaccggc cacacgctct
     3361 ggtcggacaa cttcgtcatc cccaacaagg cccgccacaa ggccaacgcc gaaaagctca
     3421 tcaactacta ctacggcccc gaggtgatgg cccaggtcgt cgcctacgtc aactacatct
     3481 cgccggtggc gggcgtgcgg gacatcgtcg tcgccgacga ccccgagacc gcggacaacc
     3541 cgttgatctt cccctctgcc gacgtgctgg cgaagtcgca cgtcttccgc ggactcaccg
     3601 aggaggagga gaccaagtac aacaaggcct tccaggccct catcggaggc tgatccatga
     3661 ccacggcggc tgcgcccggc cagcttggcg acgtcaccga cggcgcgaac gggtccgacc
     3721 tgcggctggt cgggctcacc aagcgcttcg gtgacttcac cgcggtcgag accctcgacc
     3781 tgaccatccc ggcggggtcg ttcttcgcct tgctcgggcc ctcgggttgc ggcaagacca
     3841 ccaccttgcg catggtcgcc gggctggagg agccgacgca gggccagatc ctcatcggcg
     3901 acctcgacat caccgacacc aagccctacc ggcgcccggt caacacggtg ttccagaact
     3961 acgcgctctt cccgcacctg gacatctacg agaatgtggc gttcggcttg cgccggcgcg
     4021 gccgcaagga catcgacgcc aaggtcggcg aggcgctcga gctggtgcag ctgggctcct
     4081 acgcccgacg caagccggcc cagctctccg gcggccagca gcagcgggtg gcgctggccc
     4141 gcgcgatcgt caaccgcccg gccgtgctgc tgctcgacga gccgctgggc gcgctcgacc
     4201 tcaagctgcg ccggcagatg cagatcgagc tcaagcagat ccagaccgag gtcgggatca
     4261 cgttcgtgca cgtcacgcac gaccaggaag aggccatgac catggccgac accatcgcgg
     4321 tgatggatgg cgggcgcatc gagcagctgg gcgacccgac gacgctctac gagctgccgg
     4381 ccaccacgtt cgcggccaac ttcctcggtc agtccaacct gctcgctgcc accgtggtgg
     4441 gccgagacgg tgacgacctc gtcgtcgacg tgcacggtgc ccggatggcg ctggctgcct
     4501 cccggacgcc ggtcacgaac ggggcgatgt ggctgggcat ccggccggaa aagctgcggg
     4561 tgctcacggc cgacgaggcg gtggacgggc gcaactcctt gtcgggcacg gtgaccgacg
     4621 cgtcgttcat cggcgtgtcc acccactacc tggtcaggct tccctggggc caggaggtca
     4681 cggtggtgca gcagaacgac ggcaccccgc cgctgcgccc gggcgagcgg gtgagcgtgg
     4741 catgggaacg cacccacggt ttcgggctcg acgcctccca ggacgcccac gccggtgagc
     4801 agtacgtcgt cggtg
//
LOCUS       DATIBB010000109         9563 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_978319, whole genome shotgun sequence.
ACCESSION   DATIBB010000109 DATIBB010000000
VERSION     DATIBB010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 9563)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9563)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9563
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_978319"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2033)
                     /locus_tag="VLC50_04105"
     CDS             complement(<1..2033)
                     /locus_tag="VLC50_04105"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HSF26683.1"
                     /translation="MSDLPAFRLVRRAAAASPLVLDADQCEVVSHRQGGGPMRVLGAP
                     GTGRTTVLVELAAARVERDGVAPGAVLLLAPSRRAAADLRDRVTRRLRRTVREPLARS
                     VHSYAFGLLRQAAALAGDPPPRLLAAPEQDLILRDLLAGHRAGDGRSPDWPAQLRPAL
                     TTRGFRTELRDLFARSVERGVDPAGLAALGRAHQRADWVAAATLMAEYLDVTALRSPG
                     AHDPAGLVQAAADLLESDPALLAAERDRWAVVLVDDYHDADPAAARLLGLVCGGGRDL
                     VVAGDPDSAVLDFRGGDPRLLREFCSTFRTASGADAASHTLHTSWRRPRVLVELAARV
                     TRRLPGPDDHRHLAVPDGPPGQLEVHVLRSETAEAAYLAHRLREARLRGGDAALGWSG
                     MAVIARTATRLASLRRALVSAGVPVAVSSSQLTVREQPAVVPLLTALGVVLDPEKLDP
                     ETAVALLTSPLGGADAMALRRLRQALRQEERAGGGGRASDALLVEALGDPGWLATLDH
                     RDARPGVRVATILAAGRAALARTAEGAVGDGVNAETVLWALWQASRLEESWRRTALAG
                     GPAGARADRDLDAVVALFDAAARFVDRLPAAGPAAFLDYLLGQEVPGDTLAERAPSDE
                     AVTLLTVHTASGQEWELVAVAGVYDGVWPDLRPRGSLLGSEQLVDIVSGRGSSP"
     gene            complement(2110..2439)
                     /locus_tag="VLC50_04110"
     CDS             complement(2110..2439)
                     /locus_tag="VLC50_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017596910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HSF26684.1"
                     /translation="MSAPGWQEYVEEVLDVVEAIPPGRVLTYGAIAEIVGRGGPRQVA
                     RVMSTGGGPVPWWRVVRADGRPAPCHDGEAAALLRGEGTPFRPDGTRLDMRRAFWADA
                     DASPRLT"
     gene            complement(2483..3211)
                     /locus_tag="VLC50_04115"
     CDS             complement(2483..3211)
                     /locus_tag="VLC50_04115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF26685.1"
                     /translation="MVSKARAIHPEDAQDERLATLPLSAAYTYAYLPTVLDDAGRGRD
                     QTGVLNGRLWPLRADEHPSAAMEADLAALAQAGLVCRYTVAGQAFLHLPDWAERQQPL
                     RPRPSAHPPCPTHDEPPVEATPFGRESFEESMADTFGKVAEQVNAFFGEAASNFDQDR
                     IRDSVARLVEDVTFLVNAEKAASYGERVREFLARGWAGPPSAEDSARDVPPPPRQVPP
                     GEAPSPDVPPGDTWRESTDDPTGE"
     gene            complement(3243..4433)
                     /gene="moeZ"
                     /locus_tag="VLC50_04120"
     CDS             complement(3243..4433)
                     /gene="moeZ"
                     /locus_tag="VLC50_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017627323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/sulfurtransferase MoeZ"
                     /protein_id="HSF26686.1"
                     /translation="MSLPPLVEPAAELTVDEVRRYSRHLIIPEVGMTGQKRLKNAKVL
                     VVGAGGLGSPALLYLAAAGVGTIGIIDFDVVDESNLQRQVIHGVHDLGRSKLESARDS
                     IQEINPLVEVVLHETRLDSTNALEIFAPYDLVLDGTDNFATRYLVNDTCVLLGKPYVW
                     GSIYRFDGQASVFWAEHGPCYRCVYPEAPPPGMVPSCAEGGVLGVLCASIGAVQVNEA
                     IKLIVGIGDPLVGRLKIYDALEMDYRDVRLRKDPECAICGKNPTVTELIDYEAFCGVV
                     SEAAADAVKDSTISVLTLKDMLDARERGEDDFVLVDVREPNEYEIVAIPGSVLIPKGE
                     FLDGSALEKLPQDKRVVLHCKSGGRSAEALAVVKGAGFADAVHVGGGVLAWVKQVDPS
                     LPSY"
     gene            complement(4522..5463)
                     /locus_tag="VLC50_04125"
     CDS             complement(4522..5463)
                     /locus_tag="VLC50_04125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF26687.1"
                     /translation="MTTGEWPPGGDGEHAALLEALRALPGVIGVEIDEEGGPVGAPGT
                     LRLQLAPGADDVAVAAAVNELLRRRFALAVDADRVEVVEETVSRPAAAWAPQPAAQAA
                     PAPRPEPAPTGPAEPTPQAEPTPRAEPTPEAGPRQPPRPPRPQPRAPEGPRPPDPARP
                     PHPPRPPRPTRPGPGEPTHGSRGGRFVLERATFSAAGLGTSVTVTLAYAGTPYVGTAE
                     TAATAAAASRAVAQATLRAVEAGLGGAAGGFRAEADRVDLVGTGADRVAVVIVSVLTA
                     RGAERHAGASLAAGDSRQAVIRATLAAVNRRLESFLP"
     gene            5573..5785
                     /locus_tag="VLC50_04130"
     CDS             5573..5785
                     /locus_tag="VLC50_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003992784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF26688.1"
                     /translation="MVERDFTAVDPDDAALAPEPEPVAPDEEPVSSDEHHDPLADLEA
                     PPPDEVNPADLVEQRRVVPLDEDDYR"
     gene            5809..6432
                     /locus_tag="VLC50_04135"
     CDS             5809..6432
                     /locus_tag="VLC50_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015796365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HSF26689.1"
                     /translation="MTATPDLRAGSRARLPRSARRAQLLMAAQEVFVCSGYHAAAMDE
                     IADRAGVSKPVLYQHFPGKLELYLALLDQHAAAMITAVRRALDSTTDNKQRVAAAIAA
                     YFAFVDDEGAAFRLVFESDLVNEAAVRERVDRVTHECAEAISEVIAEDAGLSAEESML
                     LAVGLVGMAQVTARWWLAQNGSLPRAAASRLVAGLAWRGIGSFPRTT"
     gene            6507..6734
                     /locus_tag="VLC50_04140"
     CDS             6507..6734
                     /locus_tag="VLC50_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007444428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3107 domain-containing protein"
                     /protein_id="HSF26690.1"
                     /translation="MEVKIGVQHAARELSLESSQTSEEIAAAVGAALESGQGLLTLDD
                     EKGRRVLIPVDKLAYVEIGEPEARKVGFGTL"
     gene            6806..7087
                     /locus_tag="VLC50_04145"
     CDS             6806..7087
                     /locus_tag="VLC50_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012922928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HSF26691.1"
                     /translation="MEASTVSIIGVIIAGIIIGLLGRLLLPGSQPIGLLVTILVGIAG
                     TLIGWWIAGLFGVEDTSGVDWLRWIISIIVAVVLVALVAGFMGRNQRRA"
     gene            complement(7124..7813)
                     /locus_tag="VLC50_04150"
     CDS             complement(7124..7813)
                     /locus_tag="VLC50_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020385741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like fold-containing protein"
                     /protein_id="HSF26692.1"
                     /translation="MTDDADGWLGDPDYRAAVVDLLGVLAYGELTAFERLADDASLAP
                     SIADKAALAAMAVSEFNHYTRLADRLVALGVDPVEAMTPFVAALEAFHERTAPSDWLE
                     GLVKAYVGDGIATDFYREVSTYLDPSTHQLVVEVLADTGHTRFAVETVRAAIEADPRV
                     AGRLALWGRRLVGEALSQAQRVAAERDALASLLVGGVDRPGADLAEFGRMLARLTEAH
                     TRRMGTLGLSA"
     gene            8015..>9563
                     /locus_tag="VLC50_04155"
     CDS             8015..>9563
                     /locus_tag="VLC50_04155"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018089427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HSF26693.1"
                     /translation="MPAPARDEEARHLTTFRDLGAHPQIAEALEALGITEAFPIQALT
                     LPLALTGADIIGQAKTGTGKTFAFGIPLLQRIIAPDDPDAGELAAPGAPQALVVVPTR
                     ELCVQVARDLETLGKARGLRVTAIYGGRAYEPQIEALRSGVDAIVGTPGRLLDLAQQR
                     HLDLSHAKVLVLDEADEMLDLGFLPDVERLIARTPETRQTMLFSATMPGQVVTLARRY
                     MRQPTHIRAADPSTGVTVENVHQHVFRAHALDKVEMLARMLQAEGRGLTMVFTRTKRT
                     AAKVSDELASRGFASAAVHGDLGQGAREQAMRAFRSGKVDVLVATDVAARGIDVDHVT
                     HVVNYQCPEDEKTYLHRIGRTARAGASGTAVTFVDWDDLPRWGLINKALGLDFAEPVE
                     TYSSSPHFFAELHIPEGTTGVLPRQLRTRAGLSAEKVEDLGETGHGPHGRKERGGRSG
                     RGDAGHSRREGQARGHDDETGASTERAERPSRSRSRRRTRNGVPVDGADVATTGSGPA
                     AAEGTGEA"
BASE COUNT         1304 a         3570 c         3510 g         1179 t
ORIGIN      
        1 ggagatgacc cgcgacccga gacgatgtcg accagctgct cggagcccag cagcgagccg
       61 cgggggcgca ggtcgggcca cacgccgtcg tagaccccgg cgaccgcgac gagttcccac
      121 tcctgcccac tggccgtgtg cacggtcagc agggtgaccg cctcgtcaga cggtgcccgc
      181 tcggccagcg tgtcgccggg cacctcctga cccagcaggt agtcgaggaa cgccgccggc
      241 ccggccgccg gcagccggtc gacgaaccgg gcggccgcgt cgaacagcgc cacgaccgcg
      301 tcgaggtcac ggtcggcccg cgcccctgcc ggcccgcctg ccagggcggt ccggcgccag
      361 gactcctcga gccggctcgc ctgccacagc gcccagagca ccgtctcggc gttgaccccg
      421 tcaccgacgg ctccttcggc ggtgcgggcc agcgccgccc gaccggccgc gaggatggtg
      481 gccacccgca ctccgggccg ggcgtcccgg tggtcgaggg tggccagcca gcccgggtcg
      541 cccagcgcct cgaccagcag cgcgtcggag gcccggccgc cccctccggc ccgctcctcc
      601 tgacggagcg cctggcgcag ccggcgcaac gccatcgcgt cggcaccgcc cagcggggag
      661 gtgagcagcg cgaccgcggt ctcggggtcg agcttctcgg ggtcgagcac cacgcccagc
      721 gcggtgagca gcgggaccac cgcgggctgc tcgcgcaccg tgagctggct cgatgacacg
      781 gcgaccggga cgccggccga gaccagcgcg cggcgcagcg aggcgagccg ggtggcggtg
      841 cgggcgatca ccgccatgcc ggaccagccg agcgcggcgt caccgccgcg cagccgggcc
      901 tcgcgcagcc ggtgggcgag gtaggccgcc tcggcggtct ccgaccgcaa aacgtgcacc
      961 tcgagctgcc cgggtggccc gtctgggacg gccaggtgac gatgatcgtc ggggccgggc
     1021 agccggcggg tcacccgcgc ggcgagctcg accagaaccc gcgggcgccg ccacgaggtg
     1081 tgcagcgtgt ggctggccgc gtcggcgccg ctcgcggtgc gaaatgtcga gcagaactcg
     1141 cgcagcagcc gtgggtcacc gccccggaag tcgagcacgg ccgagtcggg gtcgccggcc
     1201 acgaccaggt cacggccgcc gccgcagacc aggcccagca gccgggccgc ggccgggtcg
     1261 gcgtcgtggt agtcgtcgac gaggaccacc gcccagcggt cgcgctcggc ggcgagcagg
     1321 gccgggtcgg actcgagcag atcggccgcc gcctggacca gcccggcggg gtcgtgggcg
     1381 ccgggcgagc gcagcgccgt gacgtcgagg tattcggcca tcagcgtggc cgcagccacc
     1441 cagtcggctc gctggtgggc ccgcccgagc gcggccagcc cggccgggtc gacgccgcgc
     1501 tcgaccgagc gggcgaacag gtcgcgcagc tcggtgcgga acccgcgggt ggtcagcgcc
     1561 ggccgcagct gcgcgggcca gtcgggtgag cggccgtcac cggcccggtg cccggccagc
     1621 aggtcgcgca ggatcaggtc ttgctcgggg gcggccagca gccgcggtgg cgggtcgccg
     1681 gccagcgccg ccgcctgccg cagcaggccg aacgcatagg agtgcacgct gcgggccaac
     1741 ggctcgcgca cggtgcggcg cagccggcgg gtcacccggt cgcgcaggtc ggcggccgcc
     1801 cgccgggacg gtgccagcag cagcacggcg cccggggcta ctccgtcgcg ctccacccgg
     1861 gcggcggcca gctcgaccag cacggtcgtg cggccggtgc ccggcgcacc cagcacccgc
     1921 atcgggccgc cgccctggcg gtgggagacg acctcgcact ggtcggcgtc gaggacgagt
     1981 gggctcgccg cagcggcgcg gcggaccagg cggaaggcgg gcaggtctga catcgccggc
     2041 cattcgacca cgcggcaccg acagcgccgc accggccgcg tcccgagccg ggtcggggct
     2101 aggccgaggt caggtcaggc gcgggctggc gtcggcgtcg gcccagaacg cccggcgcat
     2161 gtccagccgc gtcccgtcgg gccgaaacgg ggtgccctcg ccgcgcagca gggccgcggc
     2221 ctcaccgtcg tggcacggcg cgggccggcc gtcagcgcgc accacccgcc accacggcac
     2281 cggaccgccg ccggtcgaca tcacccgggc cacctgtcgt ggtcccccgc ggccaacgat
     2341 ctcggcgatc gccccgtagg tcagcacccg gcccggcggg atcgcctcga cgacgtcaag
     2401 cacctcttcg acgtactcct gccagccggg cgcactcacg gtggccacgc tacgacgccg
     2461 tacgacgcgg tgagctcggc cctcactcgc cggtcgggtc gtcggtgctc tcgcgccagg
     2521 tgtcccccgg cggcacgtcg ggcgagggcg cctcccccgg cggcacctgc cgcggcggcg
     2581 gcggcacgtc gcgggccgag tcctccgccg acggcgggcc ggcccacccg cgggcgagga
     2641 actcgcgcac gcgttcgcca tagctggcgg ccttctcggc gttgacgagg aacgtcacgt
     2701 cttcgaccag gcgagccacc gagtcgcgga tgcggtcctg gtcgaagttg ctggcggcct
     2761 cgccgaagaa cgcgttgacc tgctcggcga ccttgccgaa cgtgtcggcc atggactcct
     2821 cgaacgactc ccggccgaag ggtgtcgcct cgaccggcgg ctcgtcgtgg gtcgggcacg
     2881 gcgggtgggc cgacggccgc ggccgcagcg gctgctggcg ctcggcccag tcgggcaggt
     2941 gcaggaaggc ctgcccggcc accgtgtagc gacacaccaa cccggcctgg gccagcgccg
     3001 ccaggtcggc ctccatcgcc gcgctggggt gctcgtcggc acgcagcggc cagagcctgc
     3061 cgttgagcac accggtctgg tccctgccgc ggccggcgtc gtcgaggacg gtcggcagat
     3121 aggcgtaggt gtaggccgca gacagcggca gcgtggccag ccgctcgtcc tgggcgtcct
     3181 cggggtggat cgcgcgtgcc ttcgagacca tgcgtcgagt ctggccgagg gcgagcctgc
     3241 tgtcagtacg acggcaggct cgggtcgacc tgcttgaccc aggccagcac gccgccgccg
     3301 acgtgcaccg cgtcggcgaa cccggcgccc ttgacgacgg ccagcgcctc ggccgagcgt
     3361 ccgccgctct tgcagtgcag caccacgcgc ttgtcctgcg gcagcttctc cagcgctgac
     3421 ccgtcgagga actcgccctt ggggatgagc accgagcccg ggatggccac gatctcgtac
     3481 tcgttgggct cgcgcacgtc gaccagcacg aagtcgtcct caccgcgctc gcgggcgtcg
     3541 agcatgtcct tgagggtcag caccgagatc gtggagtcct tgaccgcgtc ggccgcggcc
     3601 tcgctgacca cgccgcagaa cgcctcgtag tcgatgagct cggtgacggt ggggttcttg
     3661 ccgcagatcg cgcactcggg gtccttgcgc aggcgtacgt cgcggtagtc catctcgagc
     3721 gcgtcgtaga tcttgagccg accgaccagc gggtcgccga tgccgacgat gagcttgatc
     3781 gcctcgttga cctggaccgc gccgatcgag gcgcacagca caccgagcac cccgccctcg
     3841 gcgcacgagg gcaccatgcc cggcggcggc gcctcggggt agacgcagcg gtagcacggg
     3901 ccgtgctcgg cccagaacac cgaggcctgc ccgtcgaagc ggtagatcga gccccacacg
     3961 tagggcttgc cgagcaggac gcaggtgtcg ttgacgaggt agcgggtggc gaagttgtcg
     4021 gtgccgtcga ggaccaggtc gtagggggcg aagatctcga gcgcgttggt tgagtcgagc
     4081 cgcgtctcgt gcaggaccac ctcgaccagc gggttgatct cctggatcga gtcgcgggcc
     4141 gactcgagct tggacctgcc caggtcgtgc acgccgtgga tgacctgccg ctgcaggttg
     4201 gactcgtcga cgacgtcgaa gtcgatgatc ccgatggtgc ccaccccggc cgcggccagg
     4261 tagagcagcg ccggcgagcc cagaccgccg gcacccacga cgagcacctt ggcgttcttg
     4321 agccgtttct gcccggtcat gcccacctcg gggatgatca ggtggcgcga gtagcggcga
     4381 acctcgtcaa cggtgagctc agcagcgggc tcgacgagcg gcggcagcga cacgggcaga
     4441 ctcccaacgg gcgggcgttt tgtcggtgtg gtcaaccgcg tgggcccgcg gtctgttccc
     4501 aaacgatcga ggcagacccc gtcagggtag gaacgactcc aggcgccggt tgaccgcggc
     4561 cagggtggcc cggatgaccg cctggcgcga gtcgccggcc gccagcgagg cgccggcgtg
     4621 ccgttcggcg ccgcgggcgg tgaggacgct gacgatcaca acggcgaccc ggtcggcgcc
     4681 ggtcccgacc aggtcgactc gatcggcctc ggcccggaag ccgccggccg cgccccccag
     4741 gcccgcctcg accgcgcgca gggtcgcctg ggcgaccgcc cggctggcgg ccgccgcggt
     4801 ggcggccgtc tcggcggtgc cgacgtaggg cgtgccggca taggccaggg tcacggtgac
     4861 gctcgtgccg agcccggccg cggagaacgt cgcgcgctcg agcacgaacc gcccaccgcg
     4921 cgaaccgtga gtcggctcgc ccgggccggg acgggttggt cgaggcggac gcggcgggtg
     4981 gggtgggcgg gccgggtcgg gcgggcgggg tccctccggt gcccgcggct gcgggcgcgg
     5041 cggccgcggc ggctggcgag gccccgcctc gggcgtcggc tccgcgcgag gtgtcggctc
     5101 tgcctgcggt gtcggctccg ccggacccgt cggtgccggc tcaggtcgcg gagccggcgc
     5161 agcctgcgcc gccggctggg gtgcccacgc ggcggccggg cgggagaccg tctcttcgac
     5221 gacctcgacg cgatcggcgt cgacagccag cgcgaatcgc cgccgcagca gctcgttgac
     5281 cgccgccgcc acggcgacgt cgtcggcgcc aggggccagc tgcagccgga gcgtgcccgg
     5341 cgcgccgacc gggccaccct cctcgtcgat ctcgacgccg atgaccccag gcagcgcccg
     5401 cagcgcctcg agcagcgcgg cgtgctcgcc gtcgcccccc gggggccact cgcccgtcgt
     5461 caccgcagca gtgtcccagc ccgggtcggt gcgcagcgtg acggcacgcg ggagcgatca
     5521 gggcctaccc tgaagaggac ggacggcccg actcccacca cggaggacga ccatggtcga
     5581 gcgtgacttc accgctgtgg atcccgacga cgcggccctc gcgcctgagc ccgagcccgt
     5641 cgcccccgac gaggagcccg tctcctccga tgagcaccac gacccgctgg ccgatctcga
     5701 ggcgccgccc cccgacgagg tcaaccccgc cgacctcgtc gagcagcgtc gcgtcgtacc
     5761 gctcgatgag gacgactacc gctgatgggc cgacgctagg ctggcccggt gacagcgacg
     5821 cccgacctcc gcgccgggtc ccgtgcccgt ctgccgcgca gcgcgcgtcg tgcgcagctg
     5881 ctcatggcgg cccaggaggt gttcgtctgc tccggctacc acgccgcggc catggacgag
     5941 atcgccgacc gggccggtgt gagcaagccg gtgctctacc agcacttccc gggcaagctc
     6001 gagctctacc tcgcgctgct cgaccagcac gccgccgcca tgatcaccgc cgtacggcga
     6061 gcgctggact ccaccactga caacaagcag cgggtcgcgg ccgcgatcgc cgcctacttt
     6121 gcgttcgtcg acgacgaggg cgcggcgttc cggctggtct tcgagtccga cctggtcaac
     6181 gaggcagcgg tgcgcgagcg ggtcgaccgg gtcacccacg agtgtgccga ggcgatcagc
     6241 gaggtcatcg ccgaggacgc cgggttgtcc gccgaggagt cgatgctgct ggcggtcggc
     6301 ctggtcggca tggcccaggt caccgcacgc tggtggctgg cccaaaacgg ctcgttgccg
     6361 cgagcagcgg cgagccggct ggtcgccggc ctggcctggc gaggcatcgg aagcttcccg
     6421 cgcacgacct gacgacccgc gcagccacag ccagtcgtcg cgtcccacca acccaccgtc
     6481 acgcatcgcg tcactcagga ggaccagtgg aggtcaagat cggcgtccag cacgccgccc
     6541 gggagctgag cctggagagc tcgcagacga gcgaggagat cgcagccgcc gtcggcgcgg
     6601 cactcgagtc gggccagggg ctattgaccc tcgacgacga gaagggccgg cgcgtcctga
     6661 tcccggtgga caagttggct tatgtcgaga tcggcgagcc cgaggcgcgc aaggtgggtt
     6721 tcgggactct ctagacgcag cgctcgcccg gatcgatcat cgagaaaggc ccgcacggtg
     6781 cgggccggcg ctccgcgcgc cccggatgga ggcgagcacg gtgtccatta tcggagtgat
     6841 catcgccggc atcatcatcg gtctgctcgg tcgtctgctc ctgcccggca gccagccaat
     6901 cgggctgttg gtcacgatcc tcgtcggcat cgccggcacg ctcatcggct ggtggatcgc
     6961 cgggctgttc ggtgtcgagg acacctcggg ggtcgactgg ctccgctgga tcatcagcat
     7021 catcgtcgcg gtagtgctcg tcgcgctggt ggcgggcttc atgggcagga accaacggag
     7081 ggcctgagcg ccaaggccgc tgcctcgacc ccgatccagc cggtcaggcc gacaggccca
     7141 gcgtgcccat ccgccgggtg tgggcctcgg tgagccgggc gagcatccgc ccgaactccg
     7201 cgaggtcggc acctggccgg tcgacgccgc cgacgagcag gctggccaac gcgtcgcgct
     7261 cggcggccac ccgctgagcc tggctgagcg cctcaccgac caggcggcga ccccacagcg
     7321 ccagccgacc ggcgacccgc ggatcggcct cgatggccgc ccggaccgtc tcgacggcaa
     7381 agcgggtgtg gccggtgtcg gccagcacct cgacgaccag ctggtgggtc gaggggtcga
     7441 ggtaggtgct cacctcgcgg tagaagtcgg tggcgatccc atcgccgacg taggccttga
     7501 ccaacccctc gagccagtcg ctgggcgcgg tgcgctcgtg gaacgcctcg agcgccgcca
     7561 cgaacggcgt catcgcctcg accgggtcga cgcccagcgc caccagccgg tcggccaacc
     7621 gggtgtagtg gttgaactcc gagaccgcca tcgcggccag ggcggccttg tcggcgatgg
     7681 agggggccag cgaggcgtcg tcggcgaggc gctcgaacgc ggtgagctcg ccataggcga
     7741 ggacgcccag caggtcgaca accgctgccc ggtagtcggg gtcgcccagc catccatcgg
     7801 cgtcatcggt catgcccgga gcgtatccgg gccggtcgtg gccaccgccc gctagactgt
     7861 cgccgtactg gatgcccggt cggtgccccg atcggctccg accccatcta tcgcaggagt
     7921 ggccgcccgc cgccccacag tgcgatcagg cccggggagc tggcgcgccc gcacgggtgc
     7981 cctcccgtca tggaccaggt cgcggcggcg ttgcctgccg gcccctgcac gagacgaaga
     8041 ggcgagacac ctgaccacgt tccgtgacct gggggcccac ccccagatcg ccgaggccct
     8101 cgaggcgctc ggcatcaccg aggcctttcc catccaagcg ctcacgctgc cgctcgcgct
     8161 gaccggtgcc gacatcatcg gccaggccaa gaccggcacc ggcaagacgt tcgcgttcgg
     8221 catcccgctg ctgcagcgga tcatcgcgcc cgacgacccg gacgccggcg agctggccgc
     8281 gcccggtgcg ccgcaggcgc tggtcgtcgt accgacccgt gagctgtgcg tgcaggtggc
     8341 ccgcgacctg gagacgctcg gcaaggcccg cggcctgcgc gtcaccgcga tctacggtgg
     8401 gcgtgcctac gagccgcaga tcgaggcgct gcgctcgggc gtcgacgcca tcgtcggcac
     8461 ccccgggcgg ctgctcgacc tggcccagca gcgccacctc gacctgagcc acgccaaggt
     8521 gctcgtgctc gacgaggccg acgagatgct cgacctgggc ttcctgcccg acgtcgagcg
     8581 gctcatcgcg cgcacccccg agacccgcca gaccatgctg ttctcggcca ccatgcccgg
     8641 gcaggtcgtc accctggcgc gtcgctacat gcgccagccc acccacatcc gcgccgccga
     8701 cccgtcgacc ggggtcaccg tggaaaacgt gcaccagcac gtgttccggg cccacgcgct
     8761 cgacaaggtc gagatgctgg cccggatgct gcaggccgag ggccgtgggc tgaccatggt
     8821 gttcacccgc accaagcgca ccgcggccaa ggtctccgac gagctggcct cgcgggggtt
     8881 cgcctccgcc gccgtgcacg gtgacctcgg ccaaggggcc cgcgagcagg cgatgcgggc
     8941 cttccggtcg ggcaaggtcg acgtgctggt ggccaccgac gtcgcggccc gcggcatcga
     9001 cgtcgaccac gtcacccacg tggtcaacta ccagtgcccc gaggacgaga agacctacct
     9061 gcaccgcatc gggcgcaccg cccgcgccgg cgcgtcgggc accgccgtga ccttcgtcga
     9121 ctgggacgac ctgccccgct ggggcttgat caacaaggcg ctcgggctcg acttcgccga
     9181 gccggtcgag acctactcca gctcaccgca cttcttcgcg gagctgcaca tccccgaggg
     9241 caccaccggt gtgctgccgc gccagctgcg cacccgggcc ggcctgtccg ccgagaaggt
     9301 cgaggacctc ggcgagaccg ggcacggccc gcacggtcgc aaggagcgcg gcggtcgtag
     9361 cgggcgtggc gacgccggcc acagccgtcg cgagggtcag gcgcgcggcc acgacgacga
     9421 gacgggcgca tcgacggagc gggccgagcg cccaagccgc tcgcgcagcc gccgccgcac
     9481 gcgcaacggc gtcccggtcg acggcgccga cgtggccacc accgggtctg gtccggcggc
     9541 tgccgagggc accggcgagg cga
//
LOCUS       DATIBB010000110         3880 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_979968, whole genome shotgun sequence.
ACCESSION   DATIBB010000110 DATIBB010000000
VERSION     DATIBB010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3880)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3880)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3880
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_979968"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            59..892
                     /locus_tag="VLC50_04160"
     CDS             59..892
                     /locus_tag="VLC50_04160"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019137072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HSF26694.1"
                     /translation="MAASSKRARALARERHERVQKQQAARATRRRHRASVIAAVSSVL
                     VVVLLVWGVATLVGGDDHAVATPTASSASGATGTTSAAGFSCPPPASRTGSPQTFAAE
                     PPMTIAGGSSYQATITTNCGPIVVDLFADKAPHAVNSFLFLAQKGYFDGTGCHRLTTA
                     DDGLSVLQCGDPTGTGSGGPGYTFGVENPPADGVYPAGTLAMARTTDPNSNGSQFFIT
                     YADSTIPSPDGYTVFGKVVSGLDIVEKIGAAGKGEGSAPKVAVALQSIKVVTSSKGSS
                     S"
     gene            1135..2322
                     /locus_tag="VLC50_04165"
     CDS             1135..2322
                     /locus_tag="VLC50_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012852468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF349 domain-containing protein"
                     /protein_id="HSF26695.1"
                     /translation="MFVRTGDGERAVGSYPGATPDEALAYFARKYDELLAQVDLLDQR
                     VRGTDLAPKEAAASVKRLRASVAEASAVGDLDTLLARLDALGPLIDARRAEADAARAA
                     ARAEALATKERLVAEAESLAESDRWKATGDRLRELFEAWKAAPRLDRKQDDELWARFR
                     AARSTFDKRRRAHFAHLDAERADVATRKEKLVAEAEALATSTDWGATSARLRSLMDDW
                     KSAGRAGREDEDRLWARFRAAQDAFFAARNAVFAERDSGYRANLAAKEALLVEAEALL
                     PVTDLRAAKSALRSIQERWEKAGHVPRGDVERVEGRLRRVEQALRSAEDDEWRRTNPE
                     ARARAEDTVTQLRESIATLERRAASARDKGDDRAVREAEEALTARRSWLAEAERTLAE
                     FTR"
     gene            2487..3446
                     /locus_tag="VLC50_04170"
     CDS             2487..3446
                     /locus_tag="VLC50_04170"
                     /inference="COORDINATES: protein motif:HMM:NF020962.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin"
                     /protein_id="HSF26696.1"
                     /translation="MPLLFEGLPIREQDAHRRGLSRWSLRTLLERGEIRRVVRGVYVD
                     AQVPDSLLLRASALALVLPKGVVVCRSTAAWLLGIEVQEINAHLRVVEVEVCSRDGSA
                     AIRRPGVRGYSALLPDHHVTVIAGIEVTTPARTAIDLARWCERPDALAYVDALLHTGV
                     VVLDELMAVIDESFGLPWIEQAREMVDLSDAGAESGMESRMRLRYLDAGFPRPECQIA
                     ILDDAGVERYRLDCGLRRRRYGFEYDGVLFHGPERAEHDARRRAWISAQGWTIDVFNR
                     EHVLGPTLAFEEAISFALGIVPTIQPQRFRRRTYAYRRRGRAS"
     gene            complement(3536..>3880)
                     /locus_tag="VLC50_04175"
     CDS             complement(3536..>3880)
                     /locus_tag="VLC50_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008406475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HSF26697.1"
                     /translation="GHGVSVHHADCVNVKALSSQVDRLVEVEWAPSAHSVFLVAIQVE
                     ALDRSRLLSDVTRALSDQHVNILSASVTTTRDRVALSRFTFEMADPKHLGHVIKAVRG
                     VEGVYDAYRVHG"
BASE COUNT          585 a         1400 c         1360 g          535 t
ORIGIN      
        1 gcgtgggcag aggtagtccc tgtgcccgcc gggatcggcc ggatgaggag ggacgtcggt
       61 ggccgcgagc agcaagcgag cccgcgcact ggcacgggag cgccacgagc gggtccagaa
      121 gcagcaagca gcgcgggcca cccgacgacg gcaccgcgcc agcgtgatcg ccgcggtctc
      181 gtcggtgctg gtcgtcgtac tcctcgtctg gggggttgcc accctggtcg gcggcgacga
      241 tcatgcggtt gccactccga cggcctcgag cgcctccggc gcgaccggca ccaccagcgc
      301 agctgggttc agctgcccgc cgccggcaag ccgcacgggc agcccgcaga cctttgcggc
      361 cgagccgccg atgacgattg ccggcggctc gagctatcag gccaccatca ccaccaactg
      421 cgggccgatc gtcgtcgacc tgttcgccga caaggcgccg catgcggtca actcgttcct
      481 gttcctcgcc cagaagggct atttcgacgg caccggctgc caccggctga ccaccgccga
      541 cgacggcctg tccgtgctgc agtgcggtga cccgaccggg accggctccg gcgggcccgg
      601 ctacaccttc ggcgtcgaga acccgccggc cgacggcgtc tatcccgccg gcacgctggc
      661 catggcccgc accaccgacc ccaacagcaa cggcagccag ttcttcatca cctacgccga
      721 cagcaccatt ccctcccccg acggctacac ggtcttcgga aaggtcgtct ccgggctcga
      781 catcgtcgag aagatcggtg cggccggcaa gggcgagggc agcgcgccca aggtggcggt
      841 ggcgctgcag tcgatcaagg tcgtcacgtc atcgaagggg tccagctcgt gagcgacgag
      901 acgccaacca accagccgca gcctgacgag gcggctccgc cgaagccatc cgaggctgcc
      961 ccgcagccgt cgcccaccgc gacgcagccg acccccgccc cggcggtgcc gacgccggcc
     1021 caggtggccg cggcggcagc cgcctcggct gcagccgccg ctgcggcgac ggaggcctcg
     1081 gcggacctcg cggcgcgcgc cgcccgctgg gggcgagtcg gcgaggacgg caccgtgttc
     1141 gtgcgcaccg gcgacggcga gcgggcggtg ggctcctacc ccggcgccac gcccgacgag
     1201 gcgctggcct acttcgcccg caagtacgac gagctgctcg cccaggttga cctgctcgac
     1261 cagcgggtgc gcggcaccga cctggccccc aaggaggccg ctgcctcggt caagcggctg
     1321 cgggcctccg tcgccgaggc ctccgcggtc ggtgacctcg acacgctgct cgcccgcctc
     1381 gacgcgctcg gcccgctcat cgacgcccgc cgggccgagg ccgacgcggc acgcgccgcg
     1441 gcccgggccg aggcgctggc caccaaggag cgcctcgtcg ccgaggcgga gtcgctggcc
     1501 gagagcgacc gctggaaggc gaccggcgac cggctgcgcg agctgttcga ggcctggaag
     1561 gccgcccctc ggctcgaccg caagcaggac gacgagctgt gggcgcgctt ccgcgccgcg
     1621 cgctcgacct tcgacaagcg ccggcgggcc cacttcgccc acctcgacgc cgaacgcgcc
     1681 gacgtggcca cccgcaagga gaagctcgtc gccgaggctg aggcgctcgc cacctcgacc
     1741 gactggggcg ccacctcggc ccggctgcgc agcctcatgg acgactggaa gtctgccggc
     1801 cgtgccggtc gcgaggacga ggaccggttg tgggcccgct tccgggccgc ccaggacgcc
     1861 ttcttcgccg cccgcaacgc ggtcttcgcc gagcgcgact ccggctaccg ggccaacctg
     1921 gcggccaagg aggccctgct cgtcgaggcc gaggcgctgc tgccggtcac cgacctgcgg
     1981 gccgcaaagt cggcgctgcg ctcgatccag gagcgctggg agaaggccgg gcatgtgccc
     2041 cgcggcgacg tcgagcgggt cgaggggcgg ctgcgccggg tcgagcaggc gctgcgcagc
     2101 gccgaggacg acgagtggcg ccgtaccaac cccgaggcgc gcgcccgcgc cgaggacacc
     2161 gtcacccagc tgcgcgagtc gatcgcgacc ctggagcggc gcgcggccag cgcccgggac
     2221 aagggcgatg accgggcggt gcgcgaggcc gaggaagcac tgaccgcccg ccggtcgtgg
     2281 ctggccgagg ccgaacgcac cctcgccgag ttcacccgct gacacacagc cctgcactga
     2341 tcatgcgcgt ttggcggcgc cctcccctgc accccctccc ctgcgcgccc cctcccctgc
     2401 actgatcatg cgcgtttggc ggcccgccat ccccaccctg tggatagcgc gaacgcgcca
     2461 tcgaccaatc gggccatcgt gtcggcatgc cattgctgtt cgagggactg ccgattcggg
     2521 aacaagacgc ccacagacgg ggactcagcc gctggagcct gagaacgctg ctggagcggg
     2581 gcgagatccg cagagttgtg cgcggcgtgt acgtcgatgc ccaagtcccc gactcgctgc
     2641 tgttgcgggc gagtgccctg gcattggtcc tgcccaaggg cgttgtggtg tgccgcagta
     2701 cggcggcatg gttgctgggc attgaggtgc aggagatcaa tgcccacctt cgcgtggtcg
     2761 aagtggaggt ctgctctcgg gatgggtcag ctgcgattcg acgaccggga gtcaggggct
     2821 acagcgctct gctgccggat caccatgtga cggtcatcgc tggaatcgag gtcaccaccc
     2881 cggctcgcac cgccattgac ctcgcacgct ggtgcgagcg ccctgacgcg ctggcctatg
     2941 ttgacgccct gcttcacacc ggtgtcgtcg tactcgatga gctcatggcc gtcatcgatg
     3001 agtcattcgg gcttccctgg atcgaacaag cgcgcgagat ggttgatctg tccgacgccg
     3061 gcgcagagtc gggcatggag tcacggatgc gattgcgata tctcgatgcc ggcttcccaa
     3121 gacctgaatg ccagattgcg attctggacg acgctggtgt cgagcgctat cggctcgact
     3181 gcggtctccg tcgtcggcgc tatggcttcg agtacgacgg tgtgctcttc cacgggcccg
     3241 aacgagccga acatgacgcc cgtcgacgcg catggatctc cgcacagggt tggacgatcg
     3301 acgtgttcaa ccgtgagcac gtcctggggc cgacactcgc cttcgaggaa gcgatcagct
     3361 tcgctctcgg catcgtgcca accatccagc cgcaacgctt tcgacgtcgg acctacgcct
     3421 atcgccgtcg gggccgcgcc agctaagccg ctgggcccgc caaacgcgca tgatcagtgc
     3481 agggggcggg gcgaggcagc cgccaaacgc gcatgatcag tgcagggggc gggggctagc
     3541 cgtggacgcg gtaggcgtcg tagacgcctt cgacgccgcg cacggccttg atcacgtggc
     3601 ccaagtgttt ggggtcggcc atctcgaacg tgaaccgcga gagcgccacc cggtcgcggg
     3661 tggtcgtcac cgaggcggag aggatgttga cgtgctggtc tgacagcgcc cgggtgacgt
     3721 cggagagcag ccgcgagcgg tcgagggcct cgacctggat cgcgacgagg aagaccgagt
     3781 gcgccgacgg ggcccactcg acctcgacca gccggtcgac ctgcgagctc aacgccttga
     3841 cgttgacgca gtcggcgtgg tgcaccgaga cgccgtgccc
//
LOCUS       DATIBB010000111         2525 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_61238, whole genome shotgun sequence.
ACCESSION   DATIBB010000111 DATIBB010000000
VERSION     DATIBB010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2525
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61238"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            77..1411
                     /locus_tag="VLC50_04180"
     CDS             77..1411
                     /locus_tag="VLC50_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011757131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HSF26698.1"
                     /translation="MEPVLETREIYKTLDLCLRVGEVLLSSGAGAADVTATMLSLAHG
                     LGLRNADVDVTFTALSMSYQASFDDPALIQVRNVRHRDIDYEDLTEVDHLITEVLRGD
                     LDRDEARQRLAKVVSSGHRMPRWAVTLGWGTTGLGIGLLLGGDLIVVTIAFLAAAGID
                     LLKKWMSRRRLPVFYQQVAGGLFATSFAVLTAATQLPVDPSLVVTANIIMLLAGIGFM
                     GAIQDALSGFYITAGARLLEALLATAGIIAGVSGGLTLGQMLGVELSRLTPGAAGWQA
                     LPVTAFGAGLAAAAFAFACYAPLRSLLPIAAISAFGASVWYLLVIQETGPAWSSAAAA
                     VMIGLVSYSVAGRVRVPPLVVVVPAVVPLLPGLSIYRGLALMSDGDSYGIIALATAAA
                     VAIAIASGVILGEYVAQPLRREVRRLEDRLAGPRLVGPIRARAVKREKQKAA"
     gene            complement(1413..1937)
                     /locus_tag="VLC50_04185"
     CDS             complement(1413..1937)
                     /locus_tag="VLC50_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017680224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HSF26699.1"
                     /translation="MALAVCLLFDAHSELAIRRLWHRLEDDGVPTLLTHTHGAHVPHL
                     SYAVLRSFDLEKVADALDLLPDEGPMTLHFDAIGMFRRSRTWLVPSVTSDIARRQERV
                     VEAVESTGADLHQHYRPGVWVPHCTLAPRTTLDRLPTVASAVFDVLPLVTTADRAALI
                     DTSTGRQHPLDTLP"
     gene            complement(1942..2223)
                     /locus_tag="VLC50_04190"
     CDS             complement(1942..2223)
                     /locus_tag="VLC50_04190"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HSF26700.1"
                     /translation="MSDDVIARRVVVHGRVQGVFFRDSCRQQAELSRVGGWVRNEPDG
                     SVAALFEGPAHAVDAMVAWCRQGPPRARVDRLEVKEAGPSGSNVFDVLG"
     gene            complement(2220..>2525)
                     /locus_tag="VLC50_04195"
     CDS             complement(2220..>2525)
                     /locus_tag="VLC50_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020390011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HSF26701.1"
                     /translation="GHAPCDLYVDICTRPVVEERSVAAVDLDLDVLRGWTGRIWVDDE
                     DEFAEHRVRFGYPADVVELALTSCEAVRRAVELSHAPFDTETPKRWFAALEGAMMAP"
BASE COUNT          339 a          890 c          928 g          368 t
ORIGIN      
        1 ggactcgatg cggtgaccaa agacaccatc gggtgctcgg cagccaggtg gagaccagcc
       61 gggtccagcc gccaagatgg agcccgtgct ggagacgaga gagatctaca agaccctcga
      121 cctctgcctg cgcgtggggg aggtgctgct ctcgtcgggc gcaggcgccg cggacgtgac
      181 ggccacgatg ctctcgctcg cgcacggcct gggcctgcgc aacgccgacg tggacgtcac
      241 cttcaccgcg ctgtcgatga gctaccaggc gagcttcgac gacccggcac tgatccaggt
      301 ccgcaacgtg cgccatcgcg acatcgacta cgaggacctc accgaggtcg accacctcat
      361 caccgaggtg ctccggggcg acctggaccg tgacgaggcg cggcagcgtc tcgcgaaggt
      421 ggtctcgtcg ggccaccgca tgccgcggtg ggcggtgacc ctgggctggg ggacgacggg
      481 gctcgggatc ggcctgctgc tcggcggtga cctgatcgtc gtgacgatcg ccttcctcgc
      541 ggcggcgggc atcgacctgc tcaagaagtg gatgtcgcgg cgacggctgc cggtcttcta
      601 ccagcaggtg gcaggtggcc tgttcgccac gtcgttcgcc gtcctcaccg ccgccacgca
      661 gctgcccgtg gacccgtcgc tggtcgtgac ggccaacatc atcatgctgc tggccgggat
      721 cgggttcatg ggcgcgatcc aagacgccct gagcggcttc tacatcaccg ccggcgcccg
      781 cctcctcgag gcgctcctgg ccacggccgg gatcatcgcc ggtgtgagcg gcgggctgac
      841 gctcgggcag atgctgggcg tcgagctgag ccggctcacg ccgggggcgg ccggctggca
      901 ggccctcccg gtgaccgcct tcggcgccgg gctggcggca gcggccttcg cgttcgcctg
      961 ctacgcgccg ctgcgctcgc tgctcccgat cgcggcgatc tcggccttcg gcgcctccgt
     1021 ctggtacctg ctcgtcatcc aggagacggg cccggcgtgg tcctcggcgg cagcggcggt
     1081 gatgatcggt ctggtcagct actccgtggc cggccgggtg cgggtgccac ccctcgtcgt
     1141 cgtggtcccc gcggtcgtcc cgctgctgcc cggcctctcc atctaccggg gtcttgcgct
     1201 catgtcggac ggggacagct acggcatcat cgcgctcgcg acagccgccg ccgtcgcgat
     1261 cgcgatcgcc tcgggcgtga tcctcggtga gtacgtcgcc cagccgctgc gccgcgaggt
     1321 gcgccgtctc gaggaccggc tcgccggacc gcggctggtc gggccgatcc gcgcccgcgc
     1381 ggtcaagcgg gagaagcaga aggccgcgtg agtcacggca gcgtgtcgag cgggtgctgt
     1441 cggccggtgc tggtgtcgat gagggccgcc cggtcggcgg tcgtgacgag cgggaggacg
     1501 tcgaagaccg cggaggcgac cgtcggcagc cggtccagcg tcgtgcgggg tgccagggtg
     1561 cagtggggca cccacactcc ggggcggtag tgctggtgga ggtcggcccc ggtcgactcc
     1621 accgcctcca ccacccgctc ctggcgccgg gcgatgtcgg aggtcaccga cggcaccagc
     1681 cacgtgcgac tgcggcggaa catcccgatc gcgtcgaagt ggagggtcat cgggccctcg
     1741 tcgggaagca ggtcgagggc gtcggcgacc ttctcgagat cgaagctgcg caggaccgcg
     1801 taggacaggt gcgggacgtg ggcgccgtgc gtgtgggtca gcagggtggg caccccgtcg
     1861 tcctccagcc ggtgccacag ccggcggatc gcgagctcgc tgtgggcgtc gaagagcagg
     1921 cagaccgcga gtgccatcgg ctcagccgag tacgtcgaag acgttggacc cgctgggacc
     1981 ggcctctttg acctcgagcc ggtccacccg ggcgcgcggc gggccctggc ggcaccacgc
     2041 gaccatcgcg tcgaccgcgt gggcggggcc ctcgaacagg gcggccacgc tgccgtcggg
     2101 ctcgtttcgc acccagccac cgacccggga gagctctgcc tgctgacggc aggagtcgcg
     2161 gaagaacacg ccctggaccc ggccgtgcac caccacccgc cgcgcgatca cgtcgtcgct
     2221 catggggcca tcatcgcgcc ctcgagggcg gcgaaccacc gcttcggcgt ctccgtgtcg
     2281 aaaggcgcgt gcgagagctc gaccgcccgg cgtaccgcct cgcacgacgt gagcgcgagc
     2341 tccacgacat cggccgggta gccgaagcgc acgcggtgct cggcgaactc gtcctcgtcg
     2401 tcgacccaga tccggccggt ccagccccgc agcacgtcga ggtcgaggtc gacggcggcc
     2461 acggagcgtt cctcgacgac cggccgggtg cagatgtcga cgtacaggtc gcagggcgcg
     2521 tgacc
//
LOCUS       DATIBB010000112         3521 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_192516, whole genome shotgun sequence.
ACCESSION   DATIBB010000112 DATIBB010000000
VERSION     DATIBB010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3521
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_192516"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1655)
                     /locus_tag="VLC50_04200"
     CDS             complement(<1..1655)
                     /locus_tag="VLC50_04200"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014143686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma and tau"
                     /protein_id="HSF26702.1"
                     /translation="MSTALYRRYRPERFEDVIGQAHVTEPLMQALRHDRVNHAYLFSG
                     PRGCGKTTSARILARCLNCETGPTPTPCGECRSCRELASGGPGSIDVVEIDAASHGGV
                     DDARELREKAFFAPAASRYKIYIIDEAHMVTSQGFNALLKIVEEPPPHLKFVFATTEP
                     EKVIGTIRSRTHHYPFRLVPPKTLQDYLELLCEREGITVERTVLPLVVRAGGGSVRDS
                     LSVLDQLIAGAGPDGVTYPLAIGLLGFTDASLLDDVVDAFAAGDGSGVFHAVDQVVEA
                     GHNPRRFVEDLLERFRDLIVLDAVPDATSILGEIPDDQLERMRGQAGRFGTASLSRAA
                     DIINTGLTEMRGTTSPRLQLELICARVLLPGAYDDERSLHARLDRLEHRLSIAPAPLG
                     GQGSASAGEARAPVADAHPAVPARAPAVEARPDPAPARPAAPNAGPTAPAAGPAAPHA
                     GSAAPAPHAGDHAVVQDAAAPPEPAGSSAGLDLAGVRRLWPTVLDRIKDLKRLTWSLL
                     VTNAQVLSIDGGTLVLGLVNSGARDAFARGGSPEIVRQAVIDVL"
     gene            complement(1702..1798)
                     /gene="ffs"
                     /locus_tag="VLC50_04205"
     ncRNA           complement(1702..1798)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VLC50_04205"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            1896..1980
                     /locus_tag="VLC50_04210"
     tRNA            1896..1980
                     /locus_tag="VLC50_04210"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1930..1932,aa:Ser,seq:gga)
     gene            2179..2772
                     /locus_tag="VLC50_04215"
     CDS             2179..2772
                     /locus_tag="VLC50_04215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HSF26703.1"
                     /translation="MRTPAPALIPIFRSSLQARLLLRVLTDDEPRTITDLARVLEAPE
                     PTVYREVRRLMDAELVTGKRVGRSVVVTPAVDNPATAPLRQLLVVTYGPVSFLERALS
                     GVEGITEAYVYGSWAARWHGEVGSAPGDVDVLVIGRPNRDAVYDALEGMDSALGREVN
                     VTFASPERWASGDETFLKALRDRPLVRLSLGDEKRRK"
     gene            complement(2920..>3521)
                     /locus_tag="VLC50_04220"
     CDS             complement(2920..>3521)
                     /locus_tag="VLC50_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003927022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helicase HerA-like domain-containing protein"
                     /protein_id="HSF26704.1"
                     /translation="PRDVLAQLGNRVQHALRAYTPDDAKALRATVSTFPHSSYDLEEV
                     LTSLGIGEAVVTVLSERGAPTPVAWTRMRAPQSLMAPSPASVVDTVIAASALRSEYAE
                     AVDRESAYERLAARLAAVPADPSQAPAPVPSRAPSDAGWEPPAPAPGPATTVPTGLDA
                     MVDSPVFKSFARAAATVVGRELTRSIFGVGRRGRRRRRR"
BASE COUNT          489 a         1223 c         1328 g          481 t
ORIGIN      
        1 aggacgtcga tgacggcctg ccggacgatc tcggggctgc ctccgcgggc gaacgcgtca
       61 cgggcaccgg agttgaccag gcccagcacc agcgtgccgc cgtcgatgga cagcacctgg
      121 gcgttggtga ccagcagcga ccaggtcagc cgcttgaggt ccttgatccg gtcgagcacg
      181 gtgggccaga gacggcgtac gccggccagg tcgagccccg ccgaggagcc ggccggctcc
      241 ggcggcgccg ccgcgtcctg cacaactgca tgatcaccag cgtggggggc tggggcggcc
      301 gagccagcgt ggggggcggc ggggccggcg gcgggggcgg tggggccggc gttgggagcg
      361 gccgggcggg ccggagccgg atcggggcgg gcctcgacgg cgggggcgcg cgcggggacg
      421 gccgggtgag catcggcgac gggggcgcgg gcctcgcctg cggaggcact cccttgaccg
      481 ccgagcggcg ccggggcgat cgagagccgg tgctcgagcc ggtcgaggcg tgcatgcagg
      541 ctgcgctcgt cgtcgtaggc accgggcagc agcacccggg cgcagatcag ctcgagctgc
      601 agccgcggcg aggtggtgcc gcgcatctcg gtcagcccgg tgttgatgat gtcggcggcc
      661 cgcgacagcg acgcggtgcc gaaccggccg gcctgcccgc gcatccgctc gagctggtcg
      721 tcggggatct cgccgaggat gctggtcgcg tcgggcaccg cgtcgagcac gatgaggtcg
      781 cggaagcgtt cgagcaggtc ttcgacgaac cgacgcgggt tgtgcccggc ctcgaccacc
      841 tggtcgaccg cgtggaagac ccccgacccg tcgccggccg cgaacgcgtc gacgacgtcg
      901 tcgagcaggc tggcgtcggt gaacccgagc agcccgatcg ccagcgggta ggtcaccccg
      961 tcggggccgg cgccggcgat cagctggtcg agcaccgaca gcgagtcacg caccgagccg
     1021 ccgcccgcgc gcaccaccag cgggagcacc gtgcgctcga ccgtgatgcc ctcgcgctcg
     1081 cacagcagct cgaggtagtc ctgcagcgtc ttgggcggca ccagccggaa cgggtagtgg
     1141 tgggtgcgcg agcggatcgt gccgatgacc ttctcgggct cggtcgtcgc gaacacgaac
     1201 ttcaggtgcg gcggcggctc ctcgacgatc ttgagcagcg cgttgaagcc ctggctggtg
     1261 accatgtggg cctcgtcgat gatgtagatc ttgtagcgcg aggcggccgg ggcgaagaac
     1321 gccttctcgc gcagctcgcg cgcgtcgtcg accccgccgt ggctggccgc gtcgatctcg
     1381 accacgtcga tgctgcccgg cccgccgctg gccagctcgc ggcacgagcg gcactcgccg
     1441 cacggcgtcg gagtcgggcc ggtctcgcag ttgaggcagc gggccaggat gcgcgcgctg
     1501 gtcgtcttgc cgcagccgcg cgggcccgag aacaggtagg cgtggttgac ccggtcatgg
     1561 cgcagcgcct gcatcagcgg ctcggtgacg tgcgcctggc cgatgacgtc ttcgaaccgc
     1621 tcggggcggt agcggcggta gagcgcggtc gacacgcggt caccctaacc gcgccggccg
     1681 acagcccgta cggcgcgcag acgcagggac ccctcgcgca cccatcagag cccgccgacc
     1741 cttgctgcct tccggccctg ggggggttga gcgagatgac gccgcacgag gggtcgccac
     1801 ccatcctagg cgaccgccca gccctgctcg accccgcgca cgcgaccggg cgatcgactt
     1861 gggagccacc gcacgcgccc ggtaccctcc gtggcggagg attcgcctag tggcctatgg
     1921 cgcacgcttg gaaagcgtgt tgggtgcaag ccctcggggg ttcgaatccc ccatcctccg
     1981 ccggtgcgaa gggcccgtgg actgcggcga cgcagcagcg ggcccttcgt catgatcggt
     2041 tgcaggcgat ccggcggccg tgctctcagc cccgagaccg acttccgaca cgcggccgtc
     2101 atcccgcgaa ccgaggcaca cggggtgacc tctgcgcgcg aggagtgctc tcatttagcg
     2161 agagttatcc tgcgtcatat gagaacgccg gctccggcgc tgatccccat cttccggtcg
     2221 agcctgcagg cgcgactgct cttacgcgtg ctcaccgacg acgaaccgcg cacgattacg
     2281 gaccttgccc gcgtgctgga ggcgcctgaa cccaccgtgt accgcgaggt ccgccggctg
     2341 atggacgcgg agctggtcac cggcaagcgt gtcggacggt ccgtcgtcgt cacacccgca
     2401 gtggacaatc cggcgaccgc tccgctgcgc cagctgctgg tcgtcacgta cggaccggtc
     2461 tcgtttcttg agcgggcact gtcgggagtc gaggggatca cggaggccta tgtgtatggg
     2521 tcgtgggcag cgcggtggca tggcgaggtc ggctccgcgc caggcgacgt cgacgtcctg
     2581 gtgatcggaa ggccgaaccg ggatgcggtc tacgacgccc tcgaagggat ggactcggcc
     2641 ctgggtcgag aagtgaacgt caccttcgcc tctccagagc gctgggcctc tggcgacgag
     2701 acgttcctga aggcgctgcg tgaccgcccc ctggttcggc tcagtctggg tgatgagaag
     2761 cggcgcaagt aagcatccag ccggacgcgc tcgcaagaac aacccgcggc cgagcccgct
     2821 cgcgcgcggc ttcgaagcct gtccgcgagc cggggcgtct ccctcgacct ggccggcgtg
     2881 ctcggcctgg cctactgagg tcggcgcgca cgacgttggt cagcggcgac ggcgacgacg
     2941 gccgcggcgg cccacgccga agatgctccg ggtcagctcg cggccgacca cggtggccgc
     3001 ggcccgggcg aagctcttga acaccggcga gtcgaccatc gcgtctaggc cggtgggcac
     3061 ggtggtcgcc ggcccgggtg ccggcgccgg cggctcccac ccggcgtccg acggtgcccg
     3121 cgacgggacc ggtgccgggg cctgcgatgg atcagccggt acggcggcca gccgggccgc
     3181 cagccgttcg taggccgact cgcggtcgac cgcctcggcg tactcggatc gcaacgccga
     3241 cgcggcgatc acggtgtcga cgaccgacgc cggcgacggc gccatcagcg actgtggcgc
     3301 ccgcatccgg gtccaggcga ccggggtcgg ggcaccgcgc tcggacagca ccgtgacgac
     3361 cgcctcgccg atgcccagcg acgtgagcac ctcctccaag tcgtacgacg aatgcgggaa
     3421 cgtcgagacc gtcgcgcgca gtgccttggc gtcgtcgggg gtgtaggcgc gcagcgcgtg
     3481 ctggacccgg ttgcccagct gggcgagcac gtcgcggggc a
//
LOCUS       DATIBB010000113        15465 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_1902508, whole genome shotgun sequence.
ACCESSION   DATIBB010000113 DATIBB010000000
VERSION     DATIBB010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 15465)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15465)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15465
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1902508"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..558
                     /locus_tag="VLC50_04225"
     CDS             <1..558
                     /locus_tag="VLC50_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015100467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HSF26705.1"
                     /translation="DGRRGTDMGPLVTGAHRDRVASYLDAGVGEGATLVVDGREVVPD
                     GAAGGFWLGPTLFDHVSPAMTVYRDEVFGPVLSVLRVASYDEALELVNANPYGNGTAI
                     FTNDGGAARRFQNEVEVGMVGINVAIPVPVSYYSFGGWKASLFGDTHAYGTEGVHFYT
                     RGKVVTSRWPDPSHGGLNLGFPTQT"
     gene            571..1917
                     /locus_tag="VLC50_04230"
     CDS             571..1917
                     /locus_tag="VLC50_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013475393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HSF26706.1"
                     /translation="MSAPAPLTPDQIHDLDRAHVFHSWSAQAALDPMVITRAEGTHVW
                     DGAGRRYLDFGSQLVYTNLGHQHPRVVAAIKEQAERLCTIAPQHATESRSRAAAMIAE
                     VAPAGLDTVFFTNGGADAIEHAVRMARLHTGRPKVLARYRSYHGGTQQAVNLTGDPRR
                     WPSDTGTAGVVHFFGPFLYRSAFHASTEEEECERALAHLEQVITLEGPSTIAAIVLET
                     VPGTAGIMPPPPGYLAGVRQLCDRHGIVFIADEVMAGFGRTGRWFALDHWAVTPDLIT
                     FAKGVNSGYVPLGGVILSAPIASSFATRVYPGGLTYSGHPLACAAAVATITAMRDERV
                     VEHADELGREVLGPGLRELATRHSSIGEVRGLGVFWVLELVRDRATREPLAPYGGTSP
                     AMSELVSACKVRGLLPLTTFNRVHVVPPCTTSEAEAKEALAIFDEALSVADAHTVH"
     gene            complement(1925..2149)
                     /locus_tag="VLC50_04235"
     CDS             complement(1925..2149)
                     /locus_tag="VLC50_04235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF26707.1"
                     /translation="MSNGLLTRVSTMLHRPSADDRVIVAGRVACPRDVFGDVDVDRCL
                     SCPFFKGIHTSADGTQWLRCSSGQPFDRRL"
     gene            complement(2218..3159)
                     /locus_tag="VLC50_04240"
     CDS             complement(2218..3159)
                     /locus_tag="VLC50_04240"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HSF26708.1"
                     /translation="MSERAKGATVAGHRVRAVLVIVLAGLLGLTTGCASTGAGPDPAA
                     AHAVVSLDSALHDQLPAAVRDRGSLRIGTDASYAPASFFAADGRTIQGFEPDLAAAIG
                     EVLGVRIEFVVTDFDAILPRLREHEIDLAMAAITDTADRERDADFITYFTAGTSIVVQ
                     RGNPHGIIDLRDLCGTTVAVEEATVQVDLLARTQQGCDRDPIVVKAFDTNADALLQLR
                     TGRVVAVLNDYPPAAYLATDERTRGHYQLASDVQYEPGFYGIGVATDAPQLRDAVRAA
                     LDQVVRSGAYTEVLTRWGVEHGAVAQTSINSASHPGA"
     gene            complement(3156..5186)
                     /locus_tag="VLC50_04245"
     CDS             complement(3156..5186)
                     /locus_tag="VLC50_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014440591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HSF26709.1"
                     /translation="MSRTAPGARRTGSRLFVAYAAASLVPVIALGAVLAHGFRQDAVE
                     RGLDHGRAQAAVIEEMAIAPALSGHEPGAGLSAYELARLRSATDLAIFNGSVARLRVR
                     SFTGQVVFSDDGTTVGAVPSSDPAFREAAVGGTSVEIVPDSRSGQVIRVLQPIVASTN
                     GRASGVLEVYLPYDTIAASVHEQMVAMSRRLAGGLGLLYGVLALISWYTTRRLRRVAA
                     QREHQALHDHLTGLPNREWFRLRSEEAVEAARRGEHGAVVLVDLDHFKEVNDSLGHHA
                     GDELLRVVARRLSNALRTDDTVARLGGDEFGLILPQIPDEAAALTLLTRVREAVAAPI
                     ELDGLTVRTEASFGVALCPRHGTDVETLLKRADAAMYEGKRAPSGVVVYRRTASREQG
                     RSLVVQDELRRALDRDELCLHYQPKIALDSGVVCGVEALVRWAHPDRGLLPPRAFLPA
                     VERSGLIGPLTAWVLRRALTEQQEWTARGLDWVVAVNVSARNLEAGRFPTVVSELLDE
                     LAVPPARLVVEVTETALAADQDAAAAGVQALAERGIAIAIDDFGMGYTSLSQLRTLPV
                     AEVKIDRSFVSGTQAEGQDRAIVASVVDLAHGLGASATAEGVETAAVAQWLSTVGCDA
                     AQGYYYARPAPWPELMARLSVPGGADSRDGTAARSVAEPATAAGVTREGHQP"
     gene            5368..6753
                     /locus_tag="VLC50_04250"
     CDS             5368..6753
                     /locus_tag="VLC50_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012892170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HSF26710.1"
                     /translation="MGIGVSSWQLAAEVAGAGALGVVSGTALDTVLVRRLQDGDPGGH
                     VRRALAHFPAAAVAERVLERYFRPDGRAAGTPYALIPKLRLDQSRAWQELAVAASFVE
                     VWLAKQGHSGPIGVNFLEKVQMATPTAAYGAMLAGADVVLMGAGIPREIPRLLDALAE
                     HRRVSLPIDVIGARAEDRFTVDLDPVDLLGTALPPLRRPTFLAIVSAHALADYLARDP
                     LIRPDGFVVEGPPAGGHNAPPRGRLALDEDGQPVYGPRDDADTAKLAAIGLPFWLAGG
                     YGTPDRLQAALAAGAAGVQVGTVFALSRESGLRDDLRTALLDRLYGGTLRVRTDPSAS
                     PTGFPFKVAQLPGTLSEPEVYAARRRQCDLGYLRVPYLREGGTVGYRCSAEPVAAYVR
                     KGGAEADTVGKACLCNALTADIGLAQTRRSGYTEDPLVTLGADLDGAQELLKRFPDGW
                     SAADVVGWLLG"
     gene            6788..7141
                     /locus_tag="VLC50_04255"
     CDS             6788..7141
                     /locus_tag="VLC50_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016350324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HSF26711.1"
                     /translation="MSEAITVWYNPRCSKCQGAEALLAAHGVPAEKVYYLDNPPPRAE
                     IVRVLGLLGATDPRAMMRTKEPVYAELGLATADAETLIDAMAAHPVLIERPIVIRGDS
                     AVVARPPELLLDLLG"
     gene            complement(7138..7626)
                     /locus_tag="VLC50_04260"
     CDS             complement(7138..7626)
                     /locus_tag="VLC50_04260"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HSF26712.1"
                     /translation="MSALTPEQRYAAVDALPPPPPPQRMPRAGARRLGSDLLITSGRT
                     AVGPDGTLLVRGRLGGEVDLETGRRCAWQCAANVVAAARAELGSLDLIRGVPRVTVFV
                     ASAPGFVGQSLVADGATQYFHAVFGAEAGHHARSAIGVMHLPNNSPVEVEAIFDLGRA
                     SD"
     gene            complement(7631..9010)
                     /locus_tag="VLC50_04265"
     CDS             complement(7631..9010)
                     /locus_tag="VLC50_04265"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HSF26713.1"
                     /translation="MSAMTQLDLAVTNAMITVAGMGTFRGSLGVADGTVVSLNAGDAV
                     GPAREVIDADGLHVLPGVVDAHAHYGLGGDDDFTTETRSASRAGITTTLSYLLKQDLD
                     YEPAYLQMRSEGEEKSLVDFGFHFGVSSKEQAERLTLAADRFGVLSHKYFMSFKRPGE
                     GDYIGVHAVHDGILFQVMREVAKDPRVTLVVHSENIEVVWSLAEELRASGADGLAAWN
                     ESRPDFTEAHDIGTVGMFSGVTGARVFIPHVSTAAGVQAAKRFGKGRPILETCPHYLT
                     HTCDDPSLGSLGKVNPPLRPASDVEALWEAVFDGTIDLIASDHNSRKRVNKERDIWTS
                     TAGFPGQGTMLPAVLTEGVVRRGLSLERAVELLCASPARIFGRYPKKGTLRPGSDADF
                     VIVDLEAWRAPDPATWGSFSDYSLYEGQELTGWPVATYLRGRPIWTETGGWTSTPHGS
                     YVPGTTTAG"
     gene            complement(9007..9693)
                     /locus_tag="VLC50_04270"
     CDS             complement(9007..9693)
                     /locus_tag="VLC50_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007671336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HSF26714.1"
                     /translation="MSDTTPTPAIWAGAVDPADQAQYEKIGFGVKGGLGRRPAAIVID
                     VQQRTVNERYAASCGEAGRNAVPKIARVLDTARSVGAPVFFAYVAPTDSNDSSRFAVK
                     MPVLQSVDQAGYDFLDEVSPQPGDILLPKRHPSAFFGTPLVSYLVDRGIDSLIVTGAT
                     TSGCIRATVIDAFSYGYKVAVPHDAVFDRVKTSHQVNLFDINYKYGDVVGSNELVDQL
                     RALDAAPAEA"
     gene            complement(9745..10998)
                     /locus_tag="VLC50_04275"
     CDS             complement(9745..10998)
                     /locus_tag="VLC50_04275"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HSF26715.1"
                     /translation="MPEVGPGEELALVGGLVLAGEDLRLLQPGFVAMRDGVITAVGDG
                     PAPAGVRTLDITGSLLMPGMINGHTHLEDAALKELNFGVPAGVNVLFEPDGLRHTRMA
                     EIGRERVVAGMRTALEQMLASGTVAVADYRTGGAEGARALREAAAGLAVRCLIFAGHS
                     SFPVQGEAALAANTEGLTPAQLDDVAAALEVADGFAPVRVNDTTDVALAQIERLVRDA
                     GKRLSTHSSASPDYRDLSLRRTGRSDITRVVQILRPDFVVHMTVATDEEIEEIVAAGI
                     PMVMCARTMASLGRPIPPFLRAVQRGGIVGLGTDNVMMSSPDLFAEMDFLGRVARFRA
                     ADPTAVEPRQLLSAVTIDAAKAVGLDDTLGSITAGKSASLVVIDMGSANLAGSVDPLA
                     SLVERATAADIRAVLVDGAVVHGAI"
     gene            complement(11025..11906)
                     /locus_tag="VLC50_04280"
     CDS             complement(11025..11906)
                     /locus_tag="VLC50_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016909002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxaloacetate decarboxylase"
                     /protein_id="HSF26716.1"
                     /translation="MSERPNARLRRRIGGEGGPLLLPGAPNALTARLIEETGFEAVYI
                     SGAGIANTYLGAPDIGLVTLSELADHVGAVRDAVSLPIVVDGDTGFGNAINVARTVRL
                     LERAGANAIQLEDQVSPKRCGHFEGKAVISTAEMVGKIKAATDARQDDHFVLIARTDA
                     LALHGLADACDRAEAYLAAGADVAFVEAPVSRDELCSIPTRVSGPTMANIVEGGKTPP
                     LTLADLAGAGFRIVLYANTAMRAAMLAMRSVLAHLHQAGDSLALHDQIASWEERQALV
                     RKPEFDALDRRYAGGPA"
     gene            complement(11948..12667)
                     /locus_tag="VLC50_04285"
     CDS             complement(11948..12667)
                     /locus_tag="VLC50_04285"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSF26717.1"
                     /translation="MSEQVLQVEALHAIRGSSTVVRDFALSMNAGEVVGLLGANGAGK
                     TTIVDSICGFAKKESGKVRLNSRDITRLPPYKVASTGILQVSQDRELFGALSVEENLV
                     LGEQALSKTRPGEPDNLDRVFELFTRLKDRRAQRADSLSGGEQQMLAIGRALMGNPVV
                     LILDEPTSGLAPVLVKEVGHFLATMRDQGLSILLVEQNVEVALDLCDRFIVIRAGEVV
                     FEGTKADFGPDPIRALSELYV"
     gene            complement(12690..13427)
                     /locus_tag="VLC50_04290"
     CDS             complement(12690..13427)
                     /locus_tag="VLC50_04290"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HSF26718.1"
                     /translation="MSRIQPLLRAVDIVVKFGGLTAVNGVSVELFPGRITGLMGPNGA
                     GKTTFFNALSGHQLVTEGHVYMGEEEVTAVPAHGRARRGLARTFQLGGLIDDLSAVEN
                     VALGLDHGGRMSGTFISRRDLHRAAMDCLVRFDLVEVADQIGADLGAGLRRRVEVARA
                     MAAGASIVMLDEPAAGLTVTERDELARFLTTVASEGTSILITEHSSDFLFSVSDEILV
                     MNFGNELRRGAPAEIRNDPAVVAAYLG"
     gene            complement(13424..14410)
                     /locus_tag="VLC50_04295"
     CDS             complement(13424..14410)
                     /locus_tag="VLC50_04295"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HSF26719.1"
                     /translation="MTTTKLFLAERLGQRGWRLGVAAAVLALAIIGPSLLNGFQIFIV
                     SMSLCYMVATIGFNIALGWAGLLIFTGSAFFGLGAFVGGRMATLGLPTELAILVAGLA
                     GGVVGLAYGALTVKLNHYYFAITGIVLMFFLDFLYRSLPSLTGGYGGFAVPQPRLLIL
                     GNATVSSQAGQYYVGLALVVIAYVVARRLERSPLGRGWRAVRKNPGIAAGLGINVWRS
                     KLAAFTFSSIFLAVAGAWFGYLSLRFLPETYMFKELIFFFLILIVGGLGSVNGMIIGS
                     FLLVILREYLRAFPGASEMIYGTVLLLAVLVFSRGLYGAVTSRVRSLREQVL"
     gene            complement(14407..15297)
                     /locus_tag="VLC50_04300"
     CDS             complement(14407..15297)
                     /locus_tag="VLC50_04300"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HSF26720.1"
                     /translation="MSNVVLDLIQVLIAGFGQGSIYALLALGLTLIYRSTTTLNFAHG
                     ALFMLGAFVALELITAGVNYALAVVLTLVLMAALGLVIDKLAFQPLLKKDHVTQVFAT
                     VAVSFIVVGAVRFVTVDQRGMPPILGEGYLNVGDIVINTQYAMMILALILVSLVLTFF
                     FQRTRIGLIIRASTTSIRGADLVGINVKRVFGIMWAVGCMLAGLAGILAAPTLLVGPD
                     VGNTPLIVGLVAMTLGGFGSIPGAVLGGLLMGLLEATANFYVSTQLGAVSGFVVVLVL
                     LVARPEGILGVRGNRLRVSS"
BASE COUNT         2348 a         5493 c         5432 g         2192 t
ORIGIN      
        1 gacgggcgtc gcggcaccga catgggcccg ctggtcaccg gcgcgcaccg tgaccgggtg
       61 gcctcctacc tcgacgccgg cgtcggcgag ggcgccaccc tggtcgtgga cggacgcgag
      121 gtggtccccg acggcgcggc gggagggttc tggctggggc cgacgctgtt cgaccacgtc
      181 agcccggcga tgaccgtcta tcgcgacgag gtgttcggcc cggtgctgtc ggtgctgcgg
      241 gtggcgtcct acgacgaggc cctggaactc gtcaacgcca acccctacgg caacggcacc
      301 gcgatcttca ccaacgacgg cggcgccgcc cggcggttcc agaacgaggt cgaggtcggc
      361 atggtcggca tcaacgtcgc gatcccggtg ccggtgtcct actactcgtt cggcggctgg
      421 aaggcctcgt tgttcggcga cacccacgcc tacgggaccg agggcgtgca cttctacacc
      481 cgcggcaagg tggtcacctc gcgctggccc gacccctcgc acggcgggct caacctcggc
      541 ttcccgaccc agacctgaac ggagcgcccg atgagcgcac ccgcgccgct cacgcccgac
      601 cagatccacg acctcgaccg ggcccacgtc ttccactcct ggtcggccca ggctgcgctc
      661 gacccgatgg tcatcacccg cgccgagggc acgcacgtgt gggacggggc gggccggcgc
      721 tatctcgact tcggctcgca gctcgtctac accaacctcg ggcaccagca cccgcgcgtc
      781 gtcgcggcca tcaaggagca ggccgagcgg ctgtgcacaa tcgcgccgca gcacgccacc
      841 gagtcgcggt cccgggccgc cgcgatgatc gccgaggtgg ccccggccgg gctcgacacg
      901 gtgttcttca ccaacggcgg cgccgacgcg atcgagcacg cggtgcggat ggcccggctg
      961 cacaccggcc gtcccaaggt cctagcccgc taccgcagct atcacggtgg cacccagcag
     1021 gcggtcaacc tcaccggcga cccgcgccgc tggccgtcgg acaccggcac cgccggggtg
     1081 gtgcacttct tcggcccctt cctctaccgc agcgccttcc acgccagcac ggaagaggag
     1141 gagtgcgagc gcgcgctcgc ccacctggag caggtgatca cgctggaggg gccgagcacg
     1201 atcgccgcga tcgtgctcga gacggtgccg ggtacggcgg gcatcatgcc gccgccgccc
     1261 ggctatctcg ccggggtccg ccagctgtgc gaccggcacg gcatcgtgtt catcgccgac
     1321 gaggtgatgg ccgggttcgg gcgcaccggc cgctggttcg ccctcgacca ctgggcggtc
     1381 acccccgacc tcatcacgtt tgccaagggc gtcaactccg gctacgtccc gctcggcggg
     1441 gtcatcctca gcgcgccgat cgcctcctcg ttcgcgaccc gggtctatcc cggcgggctg
     1501 acctattcgg ggcacccgct ggcctgcgcg gcggcggtgg cgaccatcac cgcgatgcgc
     1561 gacgaacgcg ttgtcgagca cgccgacgag ctgggtcgcg aggtgctcgg gcccgggctg
     1621 cgcgagctcg ccacccgtca ctcatcgatc ggcgaggtgc gcgggctggg cgtgttctgg
     1681 gtgctcgagc tggtgcgcga ccgggccact cgagagccgc tggcgcccta cggcggcacc
     1741 agtccggcga tgtccgagct ggtctcggcg tgcaaggtcc gcgggctgct gccgttgacg
     1801 acgttcaacc gggtgcacgt cgtacccccg tgcacgacca gcgaggccga ggccaaggag
     1861 gcgctggcga tcttcgacga ggcgctgtcg gtcgccgacg cccacaccgt gcactgatcg
     1921 ggtctcagag acgacggtcg aagggctgcc cgctgctgca ccgcagccac tgggtgccgt
     1981 cggccgacgt atggatgccc ttgaagaacg gacagctgag acagcgatcc acgtcgacgt
     2041 caccgaagac gtcgcgcgga caggcgaccc ggccggcgac gatcacccgg tcgtcggcgg
     2101 agggtcggtg cagcatcgtc gagactctgg tcagcagccc gttgctcatg cctcgacgct
     2161 agtgatcacg cgtcggcgcc acccgggact ttcgtcccag ttgtccgggg cgttccgtca
     2221 ggcgcctggg tggctggcgg agttgatcga ggtctgcgcc accgcgccgt gctcgacgcc
     2281 ccaccgggtg agcacctcgg tgtaggcacc ggagcgcacc acctggtcga gggccgcgcg
     2341 caccgcgtcc cgcagctggg gcgcgtcggt ggccaccccg atgccgtaga agcccggctc
     2401 gtactggacg tcggaggcca gctggtagtg cccccgggtc cgctcgtcgg tggccaggta
     2461 ggccgccggc gggtagtcgt tgagcaccgc cacgacccgc ccggtccgca gctgcagcag
     2521 ggcgtcggcg ttggtgtcga aagccttgac cacgatcggg tcccggtcgc agccctgctg
     2581 ggtccgggcc agcaggtcga cctgcacggt cgcctcctcg accgcgaccg tcgtgccgca
     2641 caggtcacgc agatcgatga tcccgtgcgg gttgccgcgt tggacgacga tcgacgtacc
     2701 ggcggtgaag taggtgatga agtcggcgtc gcgctcgcgg tcggcggtgt cggtgatggc
     2761 ggccatcgcc aggtcaatct cgtgctcacg caaccggggc aggatcgcgt cgaagtcggt
     2821 gacgacgaac tcgatccgca cacccagcac ctcgccgatg gcggcggcca ggtcgggctc
     2881 gaagccctgg atcgtccgac cgtccgcggc gaagaaggat gccggggcat aggacgcatc
     2941 ggtgccgatc cgcagcgagc cgcgatcacg tacggcggcc ggcagctggt cgtgcagcgc
     3001 ggagtcgagg ctgaccaccg catgcgcggc ggcggggtcc ggcccggccc cggtcgacgc
     3061 gcacccggtc gtgagcccga gcagcccggc cagcacgatg accagcacgg cgcggacccg
     3121 gtgccccgcc acggtcgcgc ccttcgccct ctcgctcatg gctggtgccc ctctctcgtc
     3181 acgccggcgg cggtggcagg ctcagccacc gacctcgctg cggtgccgtc gcggctgtcg
     3241 gcgccgccgg gcacgctcaa ccgcgccatc agctcgggcc agggcgcggg gcgcgcgtag
     3301 tagtagccct gagccgcgtc gcagcccacc gtgctcaacc actgcgccac cgcggcggtc
     3361 tccactcctt cggcggtcgc cgaggcgccg aggccgtgcg ccaggtcgac caccgacgcg
     3421 acgatggccc ggtcctggcc ctcggcctgg gtcccgctga cgaacgaccg gtcgatcttg
     3481 acctccgcga ccggcagggt gcgcagctga gacaggctcg tgtagcccat cccgaagtcg
     3541 tcgatggcga tcgcgatccc ccgctcggcc agcgcctgca cgccggccgc ggcggcgtcc
     3601 tggtcggcgg ccagcgcggt ctcggtgacc tcgacgacca gccgggcggg cggcacggcg
     3661 agctcgtcga gcagctccga gaccacggtt gggaaccgac cggcctcgag gttgcgtgcc
     3721 gacacgttga ccgcgaccac ccagtcgagc ccacgcgccg tccactcctg ctgctcggtc
     3781 agcgcccggc gcagcaccca cgccgtcagc ggcccgatca gcccggagcg ctccacggcc
     3841 ggcagaaagg cgcggggcgg aaggaggccc cggtcggggt gcgcccaccg gacgagcgcc
     3901 tcgacgccgc agaccacgcc ggagtccagg gcgatcttgg gctggtagtg caggcacagc
     3961 tcgtcgcggt ccagggctcg ccgcagctcg tcttggacca cgagcgagcg cccctgctcg
     4021 cggctcgccg tacggcgata gaccaccacc ccggaggggg ctcgcttgcc ctcatacatc
     4081 gccgcgtcgg cccgcttgag cagcgtctcg acgtcggtgc cgtgccgcgg gcacagcgcg
     4141 accccaaagc tggcctcagt gcgcaccgtg agcccgtcga gctcgatcgg tgcggcgacc
     4201 gcctcacgga cccgggtcag cagggtcagc gcggcggcct cgtcggggat ctgcggaagg
     4261 atcaggccga actcgtcgcc cccgaggcgg gccaccgtgt cgtcggtgcg caaggcgttg
     4321 gacagccgcc gcgccaccac ccggagcagc tcgtcgccgg cgtggtgccc gagcgagtcg
     4381 ttgacctcct tgaagtggtc gaggtcgacg agcacgaccg ccccgtgctc gccgcggcgg
     4441 gccgcttcga cggcctcctc cgagcgcagc cgaaaccact cgcggttggg cagtccggtg
     4501 aggtggtcgt gcagcgcctg gtgctcgcgc tgggcggcga cccggcgcag ccggcgggtg
     4561 gtgtaccagg agatcagggc aaggacaccg tagagcaggc ccagaccgcc ggccagccga
     4621 cgggacatcg ccaccatctg ctcgtgcacg gaggcggcga tggtgtcata gggcaggtag
     4681 acctcgagca ccccgctcgc ccgcccgttc gtgctggcga cgatcggctg gagcacccgg
     4741 atcacctgtc ccgaccggga gtcgggcacg atctccaccg aggtgccccc gaccgccgcc
     4801 tcgcggaagg ccggatcgga gctgggcacc gcacccacgg tggtgccgtc gtcggagaac
     4861 acgacctggc cggtgaagct gcgcactcgc agccgcgcca ccgacccgtt gaagatcgcg
     4921 aggtcggtcg cgctgcgcaa ccgcgccagc tcgtaggcgg acaggccggc gcccggctcg
     4981 tgcccgctca gcgccggggc gatggccatc tcctcgatga ccgccgcctg cgctcggccg
     5041 tggtccagcc cgcgctcgac cgcatcctgg cggaagccgt gggccagcac ggcgccgagc
     5101 gcgatgaccg gcaccaggct ggccgcggcg taggcgacga acagccgaga ccccgtacgg
     5161 cgcgcccccg gcgcggtacg tgacatgcct cacctatcgg cgggccagcc cttcacctga
     5221 tcagttcggt cagtgccgtg atcgggacct tggtccctgg cccggccccg gtgaccatgc
     5281 caacttacgg tctcgtaact tacggccccg taacctggag gactcgtgcc gtcgccacgg
     5341 actctgcctc gactcatcca aggcggcatg ggcatcggcg tgtcctcatg gcagctggcg
     5401 gcggaggtcg ccggtgccgg cgctctcggc gtggtctccg ggacggcact ggacaccgtg
     5461 ctggtgcgcc ggttgcagga cggggacccg ggcgggcacg tccggcgagc tctcgcgcac
     5521 ttcccggccg ccgcggtggc cgagcgggtg ctcgagcgct acttccgccc cgacggccgg
     5581 gcagcgggca cgccgtacgc gctcatcccc aagctccggc tggaccagag ccgcgcctgg
     5641 caggagctgg ccgtggccgc cagcttcgtc gaggtctggc tggccaagca aggtcacagc
     5701 ggaccgatcg gcgtgaactt cctcgagaag gtccagatgg ccacacccac cgcggcctac
     5761 ggcgcgatgc tggccggtgc cgacgtcgtg ctcatgggag cgggcatccc gcgggagatc
     5821 ccgcgcctgc tcgacgcgct ggccgagcac cgccgagtca gcctgccgat cgacgtgatc
     5881 ggggcccggg ccgaggaccg gttcaccgtc gacctcgacc cggtcgacct gctgggcacc
     5941 gcgctgccgc cgctgcggcg gccgacgttc ctggcgatcg tgtcggcgca cgcgttggcc
     6001 gactacctcg cccgcgaccc actgatccgc cccgacgggt tcgtcgtcga gggcccaccg
     6061 gcgggtgggc acaacgcgcc cccacggggt cggctggccc tggacgagga cgggcagccc
     6121 gtctacgggc cccgagacga cgccgacacc gccaagctgg cggcgatcgg gctgccgttc
     6181 tggctggccg gcggctacgg cacccccgac cggctgcagg ccgctctcgc ggcgggggcc
     6241 gccggcgtgc aggtcggcac ggtctttgcg ttgtctcggg agtcggggct gcgcgacgac
     6301 ctgcgaaccg cgctgctcga ccggctctac ggcggcaccc tgcgggtgcg caccgacccg
     6361 tcggcctccc ctaccggctt cccgttcaag gtggcccagc tgcccggcac gttgtctgag
     6421 cccgaggtct atgccgcacg tcggcgccag tgcgacctcg gctacctgcg ggtgccctac
     6481 ctgcgcgagg gaggcacggt cggctatcgg tgctcggccg aaccggtggc ggcctacgtg
     6541 cgcaagggcg gcgccgaggc cgacaccgtc ggcaaggcgt gcctgtgcaa tgccttgacc
     6601 gccgacatcg ggctggccca gacccgccga agcggctaca ccgaggaccc gctggtgacg
     6661 ctcggggccg acctcgacgg cgcccaggag ctgctcaagc gctttcccga cggctggtcg
     6721 gcggccgacg tcgtcggctg gctgctcggc tgacgtggcg cagccaggcg gcggctagag
     6781 tgcgagcgtg agcgaggcca tcaccgtctg gtacaacccg cgctgctcca agtgccaggg
     6841 cgccgaggcg ctgctggccg cccacggcgt gcctgccgag aaggtctact acctcgacaa
     6901 cccgccgcca cgggccgaga tcgtgcgggt gctggggctg ctgggcgcga ccgacccgcg
     6961 ggcgatgatg cgcaccaagg agccggtgta cgccgagctg gggctggcca ccgccgatgc
     7021 cgagacgctc atcgacgcga tggccgccca cccggtgctc atcgagcggc ccatcgtgat
     7081 ccgcggcgac tcggccgtcg tcgcgcggcc ccccgagctg ctgctcgacc tgctcggcta
     7141 gtcagacgcc cgcccgaggt cgaagatcgc ctccacctcg accgggctgt tgttgggcag
     7201 gtgcatgacg ccgatcgccg accgcgcgtg gtggccggcc tctgccccga agaccgcgtg
     7261 gaaatactgg gtggccccat cggccaccag cgactgcccg acgaacccgg gcgcgctggc
     7321 cacgaagacc gtcacgcgcg ggaccccgcg gatgaggtca aggctgccca gctcggcccg
     7381 cgccgccgcc accacgtttg cggcgcactg ccaggcgcac cggcggccgg tctcgagatc
     7441 cacctccccg cccagccggc cccgcaccag cagggtgccg tcggggccca ccgcggtccg
     7501 tcccgaggtg atcagcaggt cgctgcccag gcggcgcgct cccgcgcggg gcatccgctg
     7561 gggcggcggt ggcggtggca gcgcatcgac ggcggcatag cgctgctcgg gtgtcagtgc
     7621 ggacactggc tcagcccgcc gtcgtcgtac cgggcacgta gctgccgtgc ggggtcgagg
     7681 tccagccgcc ggtctcggtc cagatcggtc ggccgcgcag gtaggtcgcc accggccagc
     7741 cggtcagctc ctgcccctca tagagggagt agtcgctgaa ggagccccac gtcgccggat
     7801 ccggcgcccg ccatgcctcc aggtccacga tcacgaagtc ggcgtccgag cccgggcgca
     7861 acgtgccctt cttcgggtag cggccgaaga tcctcgccgg cgacgcacag agcagctcga
     7921 cggcgcgctc gagcgacagc ccccgccgta cgacgccctc ggtgagcacc gcgggcagca
     7981 tggtgccctg ccccgggaag ccggccgtcg aggtccagat gtcgcgttcc ttgttgaccc
     8041 gcttgcgcga gttgtggtcg gaggcgatga ggtcgatggt gccgtcgaac accgcttccc
     8101 agagcgcctc gacgtcgctg gcggggcgca gcggcggatt gaccttgccg agcgagccga
     8161 gtgacgggtc gtcgcaggtg tgggtcaggt agtgcgggca ggtctcgagg atcggccgac
     8221 ccttgccgaa ccgcttcgcg gcctgcacac ccgcggcggt cgacacatgc gggatgaaca
     8281 cccgggcccc cgtgacgccg gagaacattc cgacggtgcc gatgtcgtgc gcctcggtga
     8341 agtccggtcg cgactcgttc caggccgcca gcccgtcggc gccgctggcc cgcagctcct
     8401 cagcgaggct ccagacgacc tcgatgttct ccgagtgcac gaccagggtg acccgagggt
     8461 ctttggcgac ctcgcgcatg acctggaaca ggatgccgtc gtgcacggcg tgcacgccga
     8521 tgtagtcgcc ctcgccgggc cgcttgaagc tcatgaagta cttgtggctg agcacgccga
     8581 accggtcggc cgccagggtc aaccgctcgg cctgttcctt ggaggagacc ccgaagtgga
     8641 agccgaagtc gacgagtgac ttctcctcac cctcgctgcg catctgcagg taggccggct
     8701 cgtagtcgag gtcctgcttg agcaggtagg acagggtcgt ggtgatcccg gcacgagacg
     8761 ccgaccgggt ctcggtggtg aagtcgtcgt caccgccgag gccgtagtgg gcgtgggcgt
     8821 cgaccacccc gggcagcacg tgcagcccgt cggcgtcgat gacctcacgg gcggggccga
     8881 ccgcgtcccc ggcgttcaac gagaccacgg tgccgtcagc gacgccgagc gaaccacgga
     8941 aggtgcccat ccccgcgact gtgatcatcg cgttggtgac ggccaggtcg agctgggtca
     9001 tggcgctcat gcctccgcag gtgcggcgtc gagggctcgc agctggtcca ccagctcgtt
     9061 ggagcccacc acgtcgccgt acttgtagtt gatgtcgaag aggttgacct ggtggctggt
     9121 cttgactcgg tcaaacaccg cgtcgtgcgg gaccgcgacc ttgtagccgt aggagaacgc
     9181 gtcgatcacc gttgctcgga tgcatccgct ggtcgttgcc ccggtgacga tcagcgagtc
     9241 aatcccccgg tccacgagat aggacacgag cggggtcccg aagaatgcac tggggtgccg
     9301 cttgggcaga aggatgtcgc cgggctgagg cgagacctcg tcaaggaagt catagccagc
     9361 ctggtccacc gactgcagca ccggcatctt gaccgcaaac ctgctggagt cgttggagtc
     9421 cgtgggcgcg acgtaggcga agaacaccgg cgcgccgacc gatcgggccg tgtcgagcac
     9481 ccgcgcgatc ttgggcaccg cattgcgtcc ggcctcgccg cagctggccg cgtagcgttc
     9541 attcacggtc cgctgctgca cgtcgatgac gatggccgcc ggccgccgac cgaggccgcc
     9601 cttgacgccg aagccgatct tctcgtactg cgcctggtcg gccgggtcga cggcgccagc
     9661 ccagatggcc ggggtgggcg tggtgtctga catgtggtcg ggctccttct ctctcgcacg
     9721 cggggtaggt cggaatgacc gggatcagat cgcgccgtgg acgacggctc cgtcgacgag
     9781 cacggcccgg atgtcggcgg cggtggctcg ctcgaccagc gaggcgagcg ggtcgaccga
     9841 gccggccagg ttggccgaac ccatgtcgat gaccaccagg ctggccgact tgccggccgt
     9901 gatggagccc agggtgtcgt ctagccccac cgccttggca gcgtcgatgg tcacggcgga
     9961 gagcagctga cgcggctcga cggcagtcgg atcggcggcg cggaagcgcg ccacccgccc
    10021 caggaagtcc atctcggcga acaggtcggg gctgctcatc atcacgttgt cggtgcccag
    10081 gcccacgatc ccgccgcgct ggacggcccg caggaacggc gggatcggcc ggccaagtga
    10141 tgccatcgtc cgggcgcaca tcaccatcgg gatgcccgcc gcgacgatct cctcgatctc
    10201 ctcgtcggtc gccactgtca tgtggacgac gaagtccggg cgcaggatct gcacgacccg
    10261 ggtgatgtcg gagcgtccgg tccgacgcaa cgacaggtcg cggtagtccg gtgacgcgct
    10321 cgaatgggtg gacagccgct tgccggcatc gcgcacgagc cgctcgatct gggcgagggc
    10381 gacgtcggtg gtgtcgttga cccgaaccgg cgcgaagccg tcggcaacct cgagggcggc
    10441 cgcgacgtcg tcgagctggg cgggtgtcag gccctcggtg ttggccgcca gggccgcctc
    10501 gccctgcacc gggaagctcg agtggcccgc gaagatcagg caccgcaccg cgaggccggc
    10561 cgcggcttcc cgcagggccc gggccccctc ggccccgccc gtgcggtagt ccgcgaccgc
    10621 aacggtgccg ctcgccagca tctgctccag cgccgtccgc atcccggcca ccacccgctc
    10681 gcgcccgatc tcggccatgc gcgtgtggcg cagcccgtcc ggctcgaaca ggacattcac
    10741 gcccgcgggc acaccgaagt tgagctcctt gagcgcggcg tcctccaggt gcgtgtgacc
    10801 gttgatcatg cccggcatga gcagggaacc cgtgatgtcg agggtgcgca ccccggcagg
    10861 tgccgggccg tcaccgaccg cggtgatcac cccgtcccgc atggctacga atcccggctg
    10921 cagcaaccga agatcctcgc cggcgagcac cagcccgcca accagtgcga gctcctcccc
    10981 cggaccaacc tcaggcatcg gtgcgcgctc ctctcgttgc gggctcatgc cggcccgccc
    11041 gcatagcgcc ggtcgagggc gtcgaactcg ggcttgcgca ccaaggcctg tcgctcctcc
    11101 cagctggcga tctggtcgtg cagcgccaac gagtcgccgg cctggtgcag gtgggcgagc
    11161 accgatcgca tcgcgagcat cgcggcgcgc atcgcggtgt tggcgtagag gacgatgcga
    11221 aagccggcac cggccaggtc ggcaagcgtc agcgggggcg tcttgccgcc ctcgacgatg
    11281 ttggccatgg tcgggccgct gacccgggtc gggatcgagc acagctcgtc gcgggagacc
    11341 ggtgcctcga cgaaggcgac atcggcgccg gccgccagat aggcctcggc acggtcacag
    11401 gcgtcggcga gcccgtgcag tgccagggcg tcggtgcggg cgatgagcac gaagtggtcg
    11461 tcctggcgag cgtcggtggc cgccttgatc ttgccgacca tctccgcggt ggagatgacc
    11521 gccttgccct cgaagtgacc acagcgcttg ggcgagacct ggtcctcgag ctggatggcg
    11581 ttggcgccgg cccgctcgag cagccgcacg gtgcgcgcca cgttgatggc gttgccgaag
    11641 ccggtgtcac cgtcgaccac gatcggcagg ctgaccgcgt cccggaccgc accgacatgg
    11701 tcggcgagct cggacagcgt gaccagcccg atgtccggtg cccccagata ggtgttggcg
    11761 atgccggcgc cggagatgta gaccgcctcg aagccggtct cctcgatcag gcgtgcggtc
    11821 agcgcgttcg gcgcgccggg cagcagcaac gggccgcctt caccaccgat ccggcggcgc
    11881 aggcgcgcat tcgggcgctc ggacactggg ctcctctcat cacggccagg tgcggcagcc
    11941 gtgcgggtca gacgtagagc tcgctcaacg cgcggatcgg gtcgggtccg aagtcggcct
    12001 tggtgccctc gaacaccacc tcgccggcgc ggatcacgat gaagcggtcg cacaggtcca
    12061 gggccacctc gacgttctgc tcgacgagca ggatggagag cccttggtcc ctcatggtcg
    12121 cgaggaagtg cccgacctcc ttgacaagca ccggggccag cccagaggtg ggttcgtcga
    12181 ggatcagcac gacggggttg cccatgaggg cgcggccgat ggcgagcatc tgctgctcac
    12241 cgccgctgag cgagtcggca cgctgggccc gtcggtcctt cagccgggtg aacagctcga
    12301 agacccggtc aaggttgtcc ggctctccgg gccgcgtctt cgacaacgcc tgctcaccga
    12361 gcaccaggtt ctcctcgacc gagagggccc cgaagagctc gcggtcctgg gagacctgca
    12421 agatcccggt ggaggccacc ttgtatggcg gcagccgggt gatgtcgcga gagttcagcc
    12481 gcaccttgcc ggactccttc ttggcgaagc cgcagatgga gtcgacgatc gtcgtcttgc
    12541 cggcaccgtt ggcccccagc aggccgacca cctcgccggc attcatgctc aaggcgaagt
    12601 cacgcaccac cgtggaggac cctcggatgg cgtgcagcgc ctcgacctgc agaacctgct
    12661 cactcatgtg atggctcacc actcccgtgt catcccaggt aggcggccac gaccgcggga
    12721 tcgttgcgga tctcggcggg ggcgccgcga cggagctcgt tgccgaagtt catgacgaga
    12781 atctcgtcgg acaccgagaa caagaagtcc gagctgtgct ccgtgatgag gatcgacgtc
    12841 ccctcggagg cgaccgtggt gagaaagcga gccagctcgt ctcgctcggt cacggtcaga
    12901 ccggcagccg gctcgtcaag catcacgatc gacgcgcctg cggccatcgc ccgggcgacc
    12961 tccacccgcc gacgcagccc ggcaccgagg tcggcgccga tctggtcggc gacctcgacg
    13021 aggtcgaagc ggaccaggca gtccatggct gcccggtgca ggtcgcgtct cgagatgaag
    13081 gtcccgctca tccgcccgcc gtggtcaaga ccgagcgcga cgttctcgac cgcgctcagg
    13141 tcgtcgatga gcccacccag ctggaaggtg cgggcgaggc ccctgcgggc ccgtccgtgg
    13201 gcgggcaccg cggtgacctc ctcttcgccc atgtagacgt gtccctcggt gaccagctga
    13261 tggccgctca aggcgttgaa gaaggtggtc ttcccggcgc cgttgggacc catcaacccg
    13321 gtgattcgcc cggggaacag ctcgacggac accccgttga ccgcggtcag gcccccgaac
    13381 ttcaccacga tgtcgacggc gcgaagcagc ggctggatgc ggctcacagg acctgctccc
    13441 tcagcgagcg gacacggctg gtgactgccc catagaggcc ccgcgagaac accagcaccg
    13501 cgagcagcag caccgtgccg tagatcatct cgctggcgcc ggggaaggcg cggaggtact
    13561 cacgcaggat gaccagcagg aacgacccga tgatcatgcc gttgaccgat cccaggcccc
    13621 ccacgatgag gatgaggaag aagaagatca gctccttgaa catgtaggtc tcgggtagga
    13681 atcgcagcga gagatagccg aaccaggctc cggcgacagc gagaaagatc gagctgaacg
    13741 tgaacgccgc cagcttggag cgccacacgt tgatcccgag ccccgcggcg atgccggggt
    13801 tcttgcggac cgcacgccag ccgcggccta gcggcgagcg ctccaaccgg cgggcgacga
    13861 cgtaggcgat caccaccaag gccagtccca cgtagtactg ccccgcctgg gaggacaccg
    13921 tcgcgttgcc gaggatgagc agcctgggct gcggcaccgc gaacccgccg taaccgccgg
    13981 tgagcgaggg caggcttcga tagaggaagt cgaggaagaa catcagcacg atgccggtga
    14041 tggcgaagta gtagtggttg agcttgacgg tcagcgcgcc ataggccaga cccaccacgc
    14101 ccccagccag cccggccacg aggatcgcca gctcggtagg cagtcccagg gttgccatcc
    14161 ggccgccgac gaaggcgccg aggccgaaga aggctgatcc agtgaagatc agcaggcccg
    14221 cccagccgag ggcgatgttg aagccgatcg tcgccaccat gtagcacagc gacatgctga
    14281 cgatgaagat ctggaagccg ttgaggagcg acggcccgat gatggccagt gccagcacgg
    14341 cggccgcgac accgagacgc cagcctcgct gaccgaggcg ctcggcgagg aagagcttcg
    14401 tcgtggtcat gagctcaccc gcagtcggtt gccacgtacc ccgaggattc cctcgggacg
    14461 ggccaccagc agcaccaaca cgacgacgaa cccggagacg gctccgagct gtgtcgacac
    14521 gtagaagttg gccgtcgcct cgagcaggcc catgagcagc ccgcccagga cggcgcccgg
    14581 aatgctgcca aacccaccga gggtcatcgc gaccaggccg acgatcaggg gggtgttgcc
    14641 gacgtcgggc ccgaccagca gcgtgggcgc cgccaggatt cccgcgagcc cggccagcat
    14701 gcagccgacc gcccacatga tgccgaagac ccgcttgacg ttgatgccga cgaggtctgc
    14761 ccctcggatg ctggtcgtgc tggcgcggat gatcagcccg atgcgagtcc gctgaaagaa
    14821 gaacgtcagg accaggctga cgagaatgag ggcgagaatc atcatggcgt actgcgtgtt
    14881 gatgacgatg tcgccgacgt tgaggtagcc ctctccgagg atcggcggca tgccccgctg
    14941 gtcgaccgtg acgaaacgca ccgcaccgac gacgatgaac gagaccgcca ccgtggcgaa
    15001 cacctgggtg acgtggtcct tcttgagcag cggctggaag gccagcttgt cgatcaccag
    15061 gccgagcgcg gccatcagca ccagggtcag caccaccgca agcgcgtagt tgacgccggc
    15121 ggtgatcagc tcgagcgcca cgaaggcgcc cagcatgaac agcgcgccgt gggcgaagtt
    15181 caacgtggtg gtcgaccggt agatcagcgt gagtccgagc gcaagcaggg catagatgct
    15241 tccctggccg aaccctgcga tgagcacctg tatcaggtct aggacaacat tgctcacgga
    15301 cagtccctcg tgagttcggg gtgccagggc gggcgcgcac gcccgccctg gcccctcggg
    15361 cgggttactt cgtcagcgcc agccagtcag accaggtgtc gacaacgacg gtcttgccgc
    15421 cgtcagactt ggggtcgagc gcgctgaagt gcagcttgat ccccg
//
LOCUS       DATIBB010000114        11255 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_1378219, whole genome shotgun sequence.
ACCESSION   DATIBB010000114 DATIBB010000000
VERSION     DATIBB010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 11255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11255
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1378219"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1932
                     /locus_tag="VLC50_04305"
     CDS             <1..1932
                     /locus_tag="VLC50_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005518936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HSF26721.1"
                     /translation="PGGQGEPAVDWAAWPYRLTPPGTRADPVLVEVVAGYLTSVEAEV
                     ETAIGRTSRSPMIRDAHDYRAGIHDRHLRKLTGRSYSALVHPVARDFPLETMKPGDVF
                     FHNDVYMSEGGIGHLPDLCVTAPVFVKDPDTGADVVVAFVQAFGHHDDIGGAVPGSMP
                     SHATSVFEEGLMVPPIRLWDAGVPNEAALKIMTRNSRMPESLAADLDAECSACLMGAR
                     RLTELFERYGLQTVEACFDAILDKTTETYRREILSKIPDGTYVWEDYAEHDGVDEPRL
                     HTQRITLTVDSSAERPMILDFTGTAPQAKGPINHCADYAEGNFLVKWLAPILRNLADT
                     PERMAELDVNEGIVPLIEMRFPPKGTLLTPVFPAPTNARTFVILRLLGVLAGVVAKAV
                     DGKMPADQETIRYTGVYGEDADGSPYLMREVLGGGSGGRYYADGEDTIHVVPDSRNLP
                     TEFSESRFPFIVERLGLALDSGGAGRYRGGLGYEKHIRMLKDAHFMSIADRSILACWG
                     VKGGKAGRPFQVTIDPGGPNEREVDALADAEPVRAGEVIRIRTTGGGGWGDPLERSYD
                     EVARDIRWGKVSFDGARADYGVVVGGTKDDPVVDEAASDALRAQLRAARPEQQPFFDR
                     GPGYARLSGGAVFADVDLL"
     gene            1978..2859
                     /locus_tag="VLC50_04310"
     CDS             1978..2859
                     /locus_tag="VLC50_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NmrA family NAD(P)-binding protein"
                     /protein_id="HSF26722.1"
                     /translation="MIKQSVLTGPVLVIGAAGKTGLAVTRALVARGVPVRAAVRGPAS
                     YDVVRAAGADAHVDVDLETGAGLAEAMAGAGAVYHLAPNVHPDEVAIAARVVAAADAA
                     GVARLVFHSVLHPHDPAMPHHLRKAEAEAVVRSGLAAWTVLQPAAYHQNLLASALAGR
                     IEVPYSIDAPFTNVDLADVAEAAARVLLEPGHARATYELAGPLTTVRGMAIEASAVLG
                     REVVAAEIDPAGWARRPAAGLPPRARTDLLAMFAAYDARGLAGNEQVLTRLLGGRPRT
                     WRDVLAQAAADRAVNRA"
     gene            complement(2846..4390)
                     /locus_tag="VLC50_04315"
     CDS             complement(2846..4390)
                     /locus_tag="VLC50_04315"
                     /inference="COORDINATES: protein motif:HMM:NF019520.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator"
                     /protein_id="HSF26723.1"
                     /translation="MTLRQLLALPVLASARPEVVHGADLLDRTVRWVHSSEIYEIGPL
                     LKGGEVLLTTGLGLVGASPDQVRGYVADLTERSVTALVLELGRTFAAVPPDMLEEARR
                     RGLVLVALHGVVPFIEVTETVHQLLIETEVASLRRLERINTELTDSLLAGGGLRPLLR
                     RIASLAGRPAMLLASDGRVVAASEADATAPHVDDPDVVRWPVHVFGVEWGHLVLCGPA
                     SADTRAVLDRGVIAVALELVRSGGLAPARTQASRELLRDIALNRFASAAELSARAAGV
                     GVVTRPGERLVAVCLRVEGARTERTVINASLEAARRTFGPALVADIDGDLLMTGSAPI
                     TDDGALRALLNRLADTIDLELAATTGGRAVAVAAGPPVDDAPALVGAVAAAREASALA
                     RALGGHRRVLLSADLGVHRLLSRLVGDPELERFVEEQLGRLIDHDARRGRELVRTLDA
                     YMSHGLSKTRTAAFLGIRRQTLYARLARVEALLGDLDLTQRERRTGLDLALVAWRLRS
                     SAVTRG"
     gene            4583..5662
                     /locus_tag="VLC50_04320"
     CDS             4583..5662
                     /locus_tag="VLC50_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014742120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family protein"
                     /protein_id="HSF26724.1"
                     /translation="MSRARPRRHTLLVLLGAAALALTGCGGATGETAPAAEVSSASTA
                     TGSAEEAPLVYGAYATPIEEPWDGVIHRALQAAADDGEIVYKHVDNIGYSGGMERTLR
                     DIAANEAPKVIFGDAFGNEDAVRKVAADFPDIAFVFGSGEPEQAPNMSVFDNWIHDPA
                     YLAGMLSAGMTKSNVLGVVGGYPVPEVNRIINAYVAGAQSVNPKVAVKVSFINSWFDP
                     AKAKQAALAQVAAGADVLFAERAGVIEAAKEKGLLSVGAMADQKDQGPESVMTSLLWD
                     MRPTVEAVLQQVKDGSYQAANLKDFSFMKNGGSSLAEINTAVAGGLPADLVAQVTAKA
                     AEITSGAFTTPVDEGTPAGSIEVKS"
     gene            5701..7212
                     /locus_tag="VLC50_04325"
     CDS             5701..7212
                     /locus_tag="VLC50_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012086303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSF26725.1"
                     /translation="MALRGIRKSFGDLLVNDGVDLSLWAGQVHALLGENGAGKTTLMR
                     ILYGLTRPDAGQIEVHGTPVTITSPKDAIGHGIGMVTQHFALVRPMTVTENVVLSEVG
                     LGPVDLQAARERVVAASERLGVRVDPDARVADLSIGEQQRVEILKALYHDCRVLVLDE
                     PTAVLVPQDVEALFATIRRLTAEGLAVMFISHKLHEVSAISDQVSVLRRGKIVESVDV
                     SKDAGIDARRLAELMVGRPTGAVRREPATDSAADGAVADPGETVLDVEGVTVAGVGRP
                     ALDAVTLRVRGGEILGVAGVSGNGQRELVDVLCGMATPVTGRVRVNDRDVTGANPRQV
                     IAAGLGRITEDRHGSVVPELSVELTLVLEELERYRRGPFLDRRRIRANAEDLIARFAI
                     KAQPADPIGSLSGGNMQKVLLARVLARDPAAVVVAQPTRGLDVGATEYVHRQLLELRG
                     RGAAILLVSEDLEELLALSDRIAVLCAGAVVGELPVADATPRRLGLLMAGAAA"
     gene            7209..8327
                     /locus_tag="VLC50_04330"
     CDS             7209..8327
                     /locus_tag="VLC50_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014742118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSF26726.1"
                     /translation="MIRLVLRGHQPRWLTSLLVLSAVVLTYLVTAGPIRAAGANPMAA
                     YERYVVTPLSSWTSFFEVLLAGTPLLFTGLAVAIAFRAGYWNIGAEGQFLAGAIAATA
                     VGIGLPDLPAVLAIPLVVLVGALAGALWALLPALLRSRLGVDEVVTTLLLNPVALLLV
                     QGLLNGPWRNPDNQFPESAPIGDGFVFPVVIPGSRVHLGFLMALVLCGIAWVVLSRTA
                     TGLRMRAVGLAPRAAAFSGVRVDRTLLGSALASGAVAGLGGVSQVCGLHHQLIGEVSA
                     GFGYTGIVVATLAMLTVSGVVLVSVLIADIVVGAETAARVLQLPPQMGQVVTATLLLT
                     TVGLLVLRRYRPVFRRTSRVSESTGEPRVSTPAGQVAQ"
     gene            8324..9220
                     /locus_tag="VLC50_04335"
     CDS             8324..9220
                     /locus_tag="VLC50_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007871776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSF26727.1"
                     /translation="MIDLLALLGAALVAATPLLYAGVGEVITERAGILNLGIEGTMYG
                     GAFAGFLVAAQTGSPWLGLLVASLVGVLAGALMGLLVVTLGVNQHVSGIGITLLLIGA
                     SEFTNRLLFGGGALTQIDKFPLLLPDVPVLGQYLLTYLGFLVVAPLAWWLLRRSGFGL
                     RVRAVGENPEAADAAGISVSRTRYAALMLGSALMALGGSFLTLAVLGTFTLNITNGRG
                     WVAIALVIFARWRVWPAVLGALLFAGVDALQLQLAITSQFAAVPRELMIALPYLAVIL
                     ALALSGRNVAYPGAYLKPYRRS"
     gene            9232..9693
                     /locus_tag="VLC50_04340"
     CDS             9232..9693
                     /locus_tag="VLC50_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012947068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HSF26728.1"
                     /translation="MTNDHTFLSVAMEQARIGFSEGGIPIGAALVVEGQVLATGRNRR
                     VQMGSAIRHGETDCLENAGRLPASVYARATIYTTLSPCDMCTGAILLYGIKRVVVGEN
                     RTFMGAEELLRSRGVEVVVLDDPECVALMERFIAERPSLWNEDIGVVDEAI"
     gene            complement(9728..10288)
                     /locus_tag="VLC50_04345"
     CDS             complement(9728..10288)
                     /locus_tag="VLC50_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014376212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HSF26729.1"
                     /translation="MGGSVTIDALATRVRAAEARAGSTRVVAIDGPSGAGKTVLAARL
                     AKALGGAPVVHLDDLYPGWDGLAAAPALLSDWVLQPLSRGEPARYRRYDWAGEEYAEW
                     HDVPAAPVLVVEGCGAGARACAPYLALLVWVDAPAEVRRRRGLARDPGYEPHWERWAA
                     QEQVMWAVDGTRERADVCLDGGPARP"
     gene            10423..>11255
                     /locus_tag="VLC50_04350"
     CDS             10423..>11255
                     /locus_tag="VLC50_04350"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012947083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HSF26730.1"
                     /translation="MAGPLEGTVVIDLTRALAGPHAGMMLGDLGARVIKVETPKGGDD
                     SRGWGPPFVGPAEDPIASYFLSCNRNKESIALDLKDEDGQETLARLVRHADVLMENFR
                     PGVLDRLGFTVGRLHELNPRLVILSISGFGHDGPEGGRAGYDQIAQGEAGLMSLTGSG
                     PDDPQRVGLPVGDLLAGMYGAYGILAALHERERSGRGQVVRTSLLAAIVGVHAYQGTR
                     WTVAGQVPRAQGNHHPSIVPYGLFRCRDGAVQIAVGSEGLWRKLCAGFGLDPAAEGMA
                     T"
BASE COUNT         1564 a         3999 c         4042 g         1650 t
ORIGIN      
        1 cccggtggcc agggggagcc ggcggtcgac tgggcggcat ggccgtaccg gctcacccca
       61 ccgggcacgc gggccgaccc cgtgctggtc gaggtggtgg ccggctacct gacctccgtg
      121 gaggcggagg tggagacggc gatcggccgg acgagccggt cgccgatgat ccgggacgcc
      181 cacgactacc gggcgggcat ccacgaccgg cacctgcgca agctcaccgg ccggtcctac
      241 tcggcgctcg tgcacccggt ggcccgcgac ttcccgctcg agacgatgaa gcccggcgac
      301 gtgttcttcc acaacgacgt ctacatgtcc gagggcggca tcggccacct gcccgacctg
      361 tgcgtcaccg ccccggtgtt cgtcaaggac ccagacaccg gcgccgacgt cgtcgtcgcg
      421 ttcgtgcagg ccttcggcca ccacgacgac atcggcggcg cggtgcccgg ctcgatgccc
      481 agccatgcga ccagcgtgtt cgaggagggc ctgatggtcc ccccgatccg gctgtgggac
      541 gccggggtgc ccaacgaggc cgcgctgaag atcatgaccc gcaactcgcg gatgcccgag
      601 tcgctggccg ccgacctcga cgccgagtgc tcggcctgcc tgatgggcgc gcgccggctg
      661 accgagctgt tcgagcgcta cggactccag acggtcgagg cctgcttcga cgcgatcctc
      721 gacaagacca ccgagaccta ccggcgcgag atcctctcga agatccctga cggcacctac
      781 gtgtgggagg actacgccga gcacgacggg gtggacgagc cgcggctgca cacccagcgg
      841 atcacgctga cggtggacag cagcgccgag cgcccgatga tcctcgactt caccggcacc
      901 gcgccgcagg ccaaggggcc gatcaaccac tgcgccgact acgccgaggg caacttcctc
      961 gtcaagtggc tggccccgat cctgcgcaac ctggccgaca cccccgagcg gatggccgag
     1021 ctcgacgtca acgagggcat cgtgccgctc atcgagatgc ggttcccgcc caagggcacg
     1081 ctgctcaccc cggtctttcc ggcgcccacc aacgcccgca ccttcgtcat cctgcgcctg
     1141 ctcggcgtgc tggccggggt ggtggccaag gcggtcgacg gcaagatgcc agccgaccag
     1201 gagacgatcc gctacaccgg cgtctacggc gaggacgccg acgggtcgcc ctacctcatg
     1261 cgcgaggttc ttggcggcgg ctccgggggg cgctactacg ccgacggcga ggacaccatc
     1321 cacgtcgtac ccgactctcg caacctgccg accgagttca gtgagagccg gttcccgttc
     1381 atcgtcgaac gcctggggct ggcgctggac tccgggggag cggggcgcta ccgcggcggg
     1441 ctcggctacg agaagcacat ccgcatgctc aaggacgctc acttcatgtc gatcgccgac
     1501 cggtcgatcc tggcctgctg gggggtcaag ggcggcaagg cgggccggcc gttccaggtc
     1561 accatcgacc cgggcggccc caacgagcgc gaggtcgacg cgctggccga cgccgagccg
     1621 gtccgggccg gcgaggtgat ccggatccgc accaccggcg gtggcggctg gggcgacccg
     1681 ctcgagcggt cctacgacga ggtggcccgc gacatccgct ggggcaaggt gtccttcgac
     1741 ggcgcccgcg ccgactacgg cgtcgtcgtc ggcggcacca aggacgaccc ggtggtcgac
     1801 gaggcggcct cggacgcgct gcgggcccag ctgcgcgcgg cgcgtccgga gcagcagccg
     1861 ttcttcgacc ggggtccggg ctacgcccgg ctctccggtg gggccgtctt cgccgacgtc
     1921 gacctgctct gacccggccc tcgccgtgat caagcaatcc gtgcccacaa tccgcccgtg
     1981 atcaagcaat ccgtgctcac cggcccggtc ctcgtcatcg gtgccgccgg caagaccggg
     2041 ctcgccgtga cgagggcgct ggtggcgcgg ggcgtgccgg tgcgggcggc ggtgcggggg
     2101 ccggcgtcgt acgacgtggt ccgggcggcc ggtgccgacg cgcacgtcga cgtcgacctt
     2161 gagaccgggg ccgggctggc ggaggcgatg gccggggccg gggcggtcta ccacctggcg
     2221 cccaacgtcc atcccgacga ggtcgcgatc gcggcacgcg tggtggccgc ggccgacgcc
     2281 gccggcgtgg cgcggctggt cttccactcg gtgctgcacc cgcacgaccc ggcgatgccg
     2341 caccacctgc gcaaggccga ggccgaggcg gtggtccggt ctggactggc ggcctggacc
     2401 gtcctgcagc ccgccgccta ccaccagaac ctgctcgcct cggcgctggc cgggcggatc
     2461 gaggtgccct actcgatcga tgcgccgttc accaacgtcg acctggccga cgtcgccgag
     2521 gccgccgccc gggtgctgct cgaacccggg cacgcacggg cgacctacga gctggccggg
     2581 ccgctgacca cggtgcgcgg catggcgatc gaggccagcg ccgtgctcgg tcgcgaggtg
     2641 gttgccgccg agatcgaccc ggctggctgg gcgcgccgac ccgccgctgg gttgccccca
     2701 cgggcgagga ccgatctcct ggcgatgttc gcggcgtacg acgctcgggg gctggccggc
     2761 aacgagcagg tgctgacccg cttgctcggc ggtcgtccgc gcacctggag ggacgtgctc
     2821 gcgcaggcgg ccgcggatcg cgccgtcaac cgcgcgtgac ggcgctgctg cgcaggcgcc
     2881 atgcgacgag tgcgaggtcg agcccggtcc ggcgctctcg ctgggtgagg tcgaggtcgc
     2941 cgagcagggc ctcgacccgc gccaggcgcg catagagggt ctgccgtcgg atgcccagga
     3001 aggccgccgt gcgggtcttg gacagcccgt gcgacatgta ggcgtcgagc gtgcgaacga
     3061 gctcgcgccc ccgtcgggcg tcgtggtcga tcagccggcc gagctgttct tcgacgaagc
     3121 gttctagctc ggggtcaccg acgaggcgag acagcagacg atggacgccg aggtcggcgg
     3181 acagcagcac gcgccggtgc cccccaagcg cgcgcgccag cgcgctggcc tcgcgggccg
     3241 cggcgaccgc gccgaccagg gctggcgcgt cgtcgacggg ggggccggcg gccaccgcga
     3301 ccgctcgtcc tcccgtcgtg gcggcaagct cgaggtcgat cgtgtccgcg agccggttga
     3361 gcaaggcgcg cagggcgccg tcgtcggtga tcggtgccga ccctgtcatg agcaggtcgc
     3421 cgtctatgtc ggcgaccagc gcggggccga acgtgcgacg ggcggcctcg aggctggcgt
     3481 tgatcacggt gcgctcggtc cgggcgcctt cgacgcgcag gcagacggcc acgagacgct
     3541 cgccgggccg ggtcacgaca cccacgcccg cggcgcgggc cgacagctcg gcggccgagg
     3601 cgaaccggtt gagcgcgatg tcgcgcagca gctcgcggct ggcctgggtc ctggccggag
     3661 ccagcccgcc gctgcgcacc agctcgaggg ctacggcgat cacgccgcga tcgagcacgg
     3721 cccgggtgtc ggcggatgcc ggcccgcaca gcaccagatg cccccattcc acgccgaaga
     3781 cgtggacggg ccatctgacg acgtctgggt cgtcgacgtg cggggcggtg gcgtcggcct
     3841 cgctggcggc gaccacgcgg ccgtcgctgg cgaggagcat ggccggtcga ccggcgaggg
     3901 aggcgatgcg ccgcagcagt ggccgcagcc ccccgccggc gagcaggctg tcggtgagct
     3961 ctgtgttgat ccgctccagc cgtcgcaggc tggcgacctc ggtctcgatc aagagctggt
     4021 ggaccgtctc ggtgacctcg atgaacggga cgacgccgtg cagggccacc aggaccaacc
     4081 ctcgccgccg agcctcctcg agcatgtccg ggggcacggc agcgaacgtg cgacccagct
     4141 ccagcaccag ggcggtgacc gagcgctcgg tcaggtcggc gacgtagccg cgaacctggt
     4201 ccggcgacgc accgacgagc ccgaggccgg tggtcagcag cacctccccg cccttgagca
     4261 gcggcccgat ctcgtagatc tccgagctgt gcacccaccg gaccgtccga tcgagcagat
     4321 ccgcaccgtg taccacctcc gggcgggccg aggcgaggac aggcagcgcc agcagctgcc
     4381 gcagggtcat cgtcacggct gggacggtgc ccgagacagc acgagacgtc aagacacgcc
     4441 gcggaaaaat ggacaatgtg tcccttgtcg ctgtattcac catgcggtgt gatcgccatc
     4501 acggtcgccg ccacagggga tggccgtcgc atggccgccg tcgtacctgc agtcgtatct
     4561 ccgtcgtcgt aaggagcgct ccatgagtcg tgccaggcca cgtcgccaca cgcttctcgt
     4621 cctgctcggg gccgcggccc tggcgctgac cggatgcggc ggggcaacgg gtgagaccgc
     4681 cccggcagcc gaggtgtcga gcgcctcgac cgccacgggg tcggcggagg aggccccgct
     4741 ggtctacggg gcctacgcga ccccgatcga ggagccctgg gacggcgtga tccaccgagc
     4801 gctgcaggct gccgccgatg acggcgagat cgtctacaag cacgtcgaca acatcggcta
     4861 ctcgggcggc atggagcgca cgctgcgaga catcgcggcc aacgaggcac cgaaggtgat
     4921 cttcggcgat gccttcggca acgaggacgc cgtgcgcaag gtggccgcgg acttccccga
     4981 catcgccttc gtgttcggct cgggtgagcc ggagcaggcg cccaacatgt cggtcttcga
     5041 caactggatc cacgaccccg cctacctcgc cggcatgctc agcgcgggga tgaccaagag
     5101 caacgtgctc ggcgtcgtcg gcggctaccc ggtgcccgag gtcaaccgga tcatcaatgc
     5161 ctacgtcgcc ggcgcccagt cggtgaaccc caaggtcgcg gtcaaggtca gcttcatcaa
     5221 cagctggttc gacccggcca aggcgaagca ggccgcgctc gcccaggtcg cggccggcgc
     5281 cgacgtcctg ttcgccgagc gggcaggcgt catcgaggcg gcgaaggaga aggggctgct
     5341 ctcggtcggc gcgatggccg accagaaaga tcaagggccc gagtcggtga tgaccagcct
     5401 gctctgggac atgcgcccca cggttgaggc agtgctccag caggtcaagg acggctccta
     5461 ccaggccgcc aacctcaagg acttcagctt catgaagaac ggtggatcct cactcgccga
     5521 gatcaacacc gccgtcgccg gcggactgcc cgccgacctg gtggcccagg tcaccgcgaa
     5581 ggctgcggag atcacgtctg gggcgttcac caccccggtc gacgagggca ccccagccgg
     5641 ttcgatcgag gtcaagagct gaccccggtg cctgagcctc acagccccca cgctccagtc
     5701 gtggcgttgc gagggatccg caagagcttt ggggacctgc tcgtcaacga cggcgtggac
     5761 ctgtccctgt gggcagggca ggtccacgcc ctgctcggcg agaacggtgc cggcaagacg
     5821 accctgatgc ggatcctcta cggcctcacg cgtccggatg ccggccagat cgaggtccat
     5881 gggacccccg tgaccatcac gtcccccaag gatgcgatcg ggcacggcat cggcatggtg
     5941 acccagcact tcgcgctggt ccggccgatg accgtgaccg agaacgtcgt gctgtccgag
     6001 gtcgggttgg gcccagtcga cctccaggca gcacgcgagc gagtcgtggc ggcatcagag
     6061 cgcctcgggg tgcgagtcga tcccgacgcg cgggtcgcgg acctgtcgat cggggagcag
     6121 caacgagtcg agatcctcaa ggcgctctac cacgactgtc gcgtgctggt gctcgacgaa
     6181 cccaccgcgg tccttgtgcc ccaggacgtc gaggcgctgt tcgcgacgat caggcgactg
     6241 acggcggagg ggctggccgt catgttcatc agccacaagc tgcatgaggt cagtgccatc
     6301 agcgatcagg tcagcgtcct gcgtcgcggc aagatcgtgg agagcgtgga cgtcagcaag
     6361 gatgcgggga tcgatgcccg ccgactcgcc gagctcatgg tgggccgccc caccggcgcc
     6421 gtacgacgtg agcccgccac ggattccgct gcggacggcg ctgttgcgga tccaggcgag
     6481 accgtgctcg acgtcgaggg tgtcaccgtc gccggtgtcg gacggccggc cctggacgcg
     6541 gtgacgctgc gcgtgcgcgg cggggagatc ctcggagtcg cgggcgtgtc tggcaatggc
     6601 cagcgcgagc tcgtcgacgt gctgtgcggc atggcgaccc cggtgaccgg ccgggtccgg
     6661 gtcaacgacc gcgacgtcac cggtgccaac ccgcgccagg tcattgccgc ggggcttgga
     6721 cgcatcaccg aggatcgcca cggcagcgtc gtgcctgagc tgtccgtcga gctcaccctc
     6781 gttctggaag agcttgagcg ctaccgacga gggccgttcc tcgaccgccg acgcatccgc
     6841 gcgaacgccg aggacctgat cgcacgcttc gcgatcaagg cccagccggc tgacccgatc
     6901 gggtcgctgt ccggggggaa catgcagaag gtgttgctcg cgcgggtgct ggcgcgagac
     6961 cccgccgcgg tggtggtggc ccagcccacg cgtgggctcg acgtcggggc caccgagtac
     7021 gtccaccgcc agctgctcga gctgcgcgga cgcggggccg ccattctgct cgtctctgaa
     7081 gacctcgaag agctgctcgc gctctccgac cggatcgccg tgctgtgcgc gggcgcggtc
     7141 gtgggcgagc tgccggtggc cgatgccacc ccgcgacggc tcgggctgct gatggccgga
     7201 gccgcggcat gatccgactt gtcctgcgcg gccaccagcc gcgctggctg acctcgctcc
     7261 ttgtgctctc ggcggtggtg ctgacctacc tggtcaccgc gggccccatc cgggcggcgg
     7321 gggccaaccc gatggccgcc tacgaacgct acgtcgtgac cccgctgtcc tcctggacct
     7381 cgttcttcga ggtgttgctg gcgggcaccc cgttgctgtt cacgggcctc gccgtggcga
     7441 tcgcctttcg cgccggctat tggaacatcg gcgcggaggg gcagttccta gccggagcca
     7501 tcgccgcgac ggccgtcggc atcggcctgc ctgacctgcc ggcggtgctg gcgatcccgc
     7561 tggtcgtgct ggtcggagcc ctcgcggggg cgttgtgggc cttgctgcct gctctgctgc
     7621 gctcgcgtct gggcgtcgac gaggtggtga ccacgttgct gctcaacccc gtggcgctgc
     7681 tcttggtcca gggtctgctc aacggtccgt ggcgcaaccc tgacaaccag ttccccgagt
     7741 cggcccccat cggcgacgga ttcgtcttcc ccgtggtcat tcccggctcg cgcgtgcatc
     7801 tgggctttct catggctctg gtcctgtgcg ggatcgcctg ggtggtcctc tcgcgcacgg
     7861 ccacggggct gcggatgcgt gcggtcgggc tcgccccccg tgccgcggca ttcagtgggg
     7921 tccgggtgga tcgcaccttg ctcggctcgg cgctcgcgag cggagcagtc gcgggcctgg
     7981 gtggcgtgag ccaggtctgc gggctgcacc atcagctcat cggtgaggtc tccgccggct
     8041 tcggctacac cgggattgtc gtggccaccc tggcgatgct gaccgtcagc ggcgtggtcc
     8101 tcgtgtcggt gctgatcgcc gacatcgtcg tcggggctga gactgcggcc cgggtgctgc
     8161 agctgcctcc gcagatgggt caggtggtca cggcaacgct gctgctgacc accgtcggcc
     8221 tactggtgct gcgccgctac cgacccgtgt tccgccgtac ctctcgggtg tccgagtcca
     8281 cgggtgagcc acgcgtgtcg accccagccg ggcaggtggc gcaatgatcg atctgctcgc
     8341 actgctgggc gctgcgcttg ttgcggcgac gccgctcctc tacgcgggcg tcggcgaggt
     8401 catcacggag cgggccggca tcctcaatct cgggatcgaa ggcacgatgt acggcggtgc
     8461 cttcgccgga ttcctcgtcg cggctcagac cggatcaccg tggctcgggc tgcttgtggc
     8521 ctcgctcgtc ggggtgctcg cgggtgcgct gatggggttg ctcgtcgtga ctctcggcgt
     8581 caaccagcat gtctccggca tcgggatcac gctgctgctc atcggcgcga gcgagttcac
     8641 caaccggctg ctcttcggcg ggggtgcgct cacgcagatc gacaagttcc ccctgctgct
     8701 gcccgatgtg ccggtgctgg ggcagtacct gctcacctat ctcggattcc tcgtcgtggc
     8761 gcccctggcc tggtggctgc tgcgccgatc cggcttcggc ctgcgggtgc gagccgtggg
     8821 ggagaacccc gaggccgctg acgccgccgg catcagcgtc tcgcgcaccc gctatgccgc
     8881 gctgatgctc ggcagcgcgc tgatggcgct gggcgggtcg ttcctcacac tggcggtgct
     8941 gggcacgttc acgctcaaca tcaccaacgg ccggggatgg gtggccatcg ccttggtcat
     9001 cttcgcccgc tggcgggtgt ggccggccgt gctcggggcc ctgctcttcg ccggtgtcga
     9061 cgcgctgcag ctgcagctcg ccatcacgtc gcagttcgcc gcggtgccgc gtgagctgat
     9121 gatcgcactg ccctacctcg cggtgatcct tgcgctggcg ctgtccggcc gcaacgtcgc
     9181 ctacccgggc gcctacctca agccttaccg acggagctga ctggagctga catgaccaac
     9241 gaccacactt tcctctcggt ggccatggag caggcccgga tcgggttctc cgaggggggt
     9301 atcccgatcg gcgcagcgct cgtcgtggag ggccaggtgc tggccaccgg gcggaaccgt
     9361 cgcgtgcaga tgggcagtgc gattcggcac ggcgagacag actgcctgga gaacgcgggc
     9421 cggctgccgg cgagcgtcta cgcccgggcg accatctaca ccacgctgtc cccgtgcgac
     9481 atgtgcaccg gtgcgatctt gctctatggg atcaagcggg tggtggtcgg tgagaaccgc
     9541 accttcatgg gggccgagga gctgctccgg tcgcggggcg tagaggtcgt ggtgctcgac
     9601 gaccccgagt gcgtcgcgct gatggagcgg ttcatcgccg aacggccgag cctgtggaac
     9661 gaggacatcg gcgtggtcga cgaggccatc tgacgccggc cgtcgcgggg aggtccaccg
     9721 ccggatctca gggccgggcg gggccgccat cgaggcacac gtcggcgcgc tctcgggtgc
     9781 cgtccaccgc ccacatcacc tgctcctgtg cggcccagcg ctcccagtgg ggctcatagc
     9841 ccgggtcgcg agccagacca cggcgacgcc gcacctccgc cggcgcgtcg acccagacca
     9901 gcagcgcaag gtagggcgcg caggcacggg cgccggcccc gcagccctcg acgaccagca
     9961 ccggggccgc gggcacgtcg tgccactcgg cgtactcctc gccggcccag tcgtagcgcc
    10021 ggtagcgcgc gggctcgccg cgggagagcg gctggagcac ccagtcggac agcagcgccg
    10081 gcgcggcggc cagcccgtcc cagcccgggt agaggtcgtc caggtgcacg accggcgctc
    10141 cgccgagcgc tttggccagc cgggccgcca gcaccgtctt gcccgctccc gacgggccgt
    10201 cgatggccac gaccctggtg gagcccgcgc gagcctcggc cgcacgcacc cgcgttgcca
    10261 gcgcgtcgat cgtcacggat ccgcccacga tcgcgaccgt agccgttctc gcacaacgcg
    10321 aaggcgcccg gcatggcgag tcccgacgac gactccccgg cgccggcatg gcaccctcgg
    10381 caggacgaat gaccgggaac gaccaaggta gggagcatcg gcgtggctgg accgctcgag
    10441 ggcacggtgg tcatcgactt gacgcgagcg ttggccggcc cgcacgccgg gatgatgctc
    10501 ggcgacctcg gcgcccgcgt gatcaaggtt gagaccccca agggcggcga cgactcccgc
    10561 ggctggggcc cgccgttcgt cggcccggcc gaggacccca tcgcctccta cttcctgtcc
    10621 tgcaaccgca acaaggagtc gatcgccctc gacctcaagg acgaggacgg ccaagagacg
    10681 ctggcccggc tggtccgcca cgccgacgtg ctgatggaga acttccgtcc cggggtgctc
    10741 gaccggctcg ggttcaccgt gggccgcctg cacgagctca acccccggct ggtgatcctc
    10801 tcgatcagcg ggttcggcca cgacgggccc gagggcgggc gggccggcta cgaccagatc
    10861 gcccagggtg aggccgggct gatgtcactg accggctccg gccccgacga cccgcagcgg
    10921 gtcgggctgc cggtcggtga cctgctggcc ggcatgtacg gcgcctacgg catcctcgcc
    10981 gccctgcacg agcgcgagcg cagcggccgc ggccaggtcg tgcgcacctc gttgctggcc
    11041 gccatcgtcg gggtgcacgc ctaccagggc acccggtgga cggtggccgg ccaggtgcca
    11101 cgcgcgcagg gcaaccacca cccctcgatc gttccctacg ggctcttccg ctgccgcgac
    11161 ggtgccgtgc agatcgctgt cggcagcgag gggctctggc gcaagctctg cgccggcttc
    11221 gggctcgacc cggcggccga gggcatggcc accaa
//
LOCUS       DATIBB010000115         2756 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_1508731, whole genome shotgun sequence.
ACCESSION   DATIBB010000115 DATIBB010000000
VERSION     DATIBB010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2756)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2756)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2756
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1508731"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1757)
                     /locus_tag="VLC50_04355"
     CDS             complement(<1..1757)
                     /locus_tag="VLC50_04355"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012889333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamine synthetase]
                     adenylyltransferase/[glutamine
                     synthetase]-adenylyl-L-tyrosine phosphorylase"
                     /protein_id="HSF26731.1"
                     /translation="MSAARRQTGLGALARHGFADAQAAERLLVDPVLAGQPANRDRLV
                     EALGASADPDLALRGLVRLAEAAPDRDALMAALREDDGLRERLLAVLGFSAALGEHLA
                     RHPEHWHVLREDGRRPAPDDVRRMLLRAVGADPDAAAPVAPDPGLATDALRVAYRRSQ
                     LGLAARDLTERMGLEEVAGELADLAAATVEAALAVARGELPADATPARLAVIGMGKCG
                     GCELNYVSDVDVVFVAAPADDQADADTERAALATATRLAAGLMRVCSATTGEGTIWPV
                     DAALRPEGKAGPLVRTLASHLAYYQRWAKTWEFQALLKARPIAGDLALGHAYVEAVAP
                     LVWAAAAREHFVEDVQAMRRRVEEHVPADVADRQLKLGPGGLRDVEFAVQLLQLVHGR
                     TDESLRSATTLDALAALAAGGYVGRDDAAELGEAYRFLRTLEHRIQLHRLRRTHVVPE
                     RDEDLRRLGRSMGMRRDPVAELTTQWRRHAREVRRLHEKLFYRPLLLAVAHLAPDEVR
                     LTPAEASARLGALGYADPDGALRHIEALTSGVSRRAAIQRTLLPVMLGWFADAPDPDG
                     GLLAFRRVSEALGATHWYL"
     gene            complement(1782..>2756)
                     /locus_tag="VLC50_04360"
     CDS             complement(1782..>2756)
                     /locus_tag="VLC50_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013151975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HSF26732.1"
                     /translation="GFTFYTHPEIEFFLFEKEPAPDGSPAPIDSSGYFDHTPHGIGHD
                     FRRSAITMLESMGISVEFSHHEGAPGQQEIDLRYADALSTADNIMTFRLVVKEVALEQ
                     GVYASFMPKPFSDHPGSGMHTHLSLFEGDRNAFHEAGAPYQLSKVGRSFIAGLLHHAA
                     EITAVTNQWVNSYKRLSGGGEAPAYICWGHNNRSALIRVPMYKPHKGQSTRVELRSPD
                     SACNPYLAFAVMLAAGLKGVEQGYELPEGAEDDVWALTDRERRVLGITPLPTSLAEAI
                     EAMEASELVAETLGEHVFDFVLRNKRAEWADYRRQVTPFELNRYLPVL"
BASE COUNT          356 a          993 c         1039 g          368 t
ORIGIN      
        1 cttagatacc agtgggtggc cccgagcgcc tcgctgaccc ggcggaaggc gagcagcccg
       61 ccgtcggggt cgggcgcatc ggcgaaccag ccgagcatca ccggcagcag ggtgcgctgg
      121 atcgcggccc ggcggctgac ccccgaggtg agcgcctcga tgtggcgcag cgccccgtcg
      181 gggtcggcgt agccgagcgc gcccagccgg gccgaggcct ccgccggggt gagccggacc
      241 tcgtccggcg ccaggtgcgc cacggccagc agcagcgggc ggtagaagag cttctcgtgc
      301 agccggcgca cctcccgggc gtgccggcgc cactgcgtgg tcagctcggc gaccgggtcg
      361 cggcgcatcc ccatcgaccg gcccagccgg cggaggtcct cgtctcgctc gggcaccaca
      421 tgggtacggc gcagccggtg cagctggatc cggtgctcga gggtgcgcag gaaccggtag
      481 gcctcaccca gctcggccgc gtcgtcgcgg ccgacgtagc cgccggcggc cagcgccgcg
      541 agcgcgtcca gcgtggtcgc gctgcgcaac gactcgtcgg tgcgaccgtg cacgagctgg
      601 agcagctgga cggcgaactc gacgtcgcgc agcccgccgg gcccgagctt gagctgacgg
      661 tcggcgacgt cggcgggcac gtgctcctcg acccgccggc gcatcgcctg cacgtcttcg
      721 acgaagtgct cgcgggcggc cgcggcccac accagcggcg ccaccgcctc gacgtaggcg
      781 tgcccgagcg ccaggtcgcc ggcgatcggg cgggccttga gcagcgcctg gaactcccaa
      841 gtcttggccc accgctggta gtaggcgagg tggctggcca gcgtccgcac cagcgggcca
      901 gccttgccct ccgggcgcag cgccgcgtcg accggccaga tcgtgccctc ccccgtcgtg
      961 gccgagcaga cccgcatgag gccggccgcc aagcgggtcg cggtggccag cgccgcgcgc
     1021 tcggtgtcgg cgtcggcctg gtcgtctgcc ggggccgcga cgaagacgac gtcgacgtcg
     1081 ctgacgtagt tgagctcgca gccgccgcac ttgcccatgc cgatgaccgc cagccgggcc
     1141 ggcgtggcat cggcgggcag ctcgccacgg gccaccgcga gggccgcctc gaccgtggcc
     1201 gcggccaggt cggccagctc gccggcgacc tcctcgaggc ccattcgttc ggtgaggtcg
     1261 cgggcggcca gcccgagctg cgaccggcgg taggcgaccc gcagcgcgtc ggtggccagg
     1321 cccgggtcgg gggcgaccgg ggcggcggcg tcggggtccg cgccgacggc gcgcagcagc
     1381 attcgtcgta cgtcgtcagg ggctgggcgc ctgccgtcct cccgcagcac gtgccagtgc
     1441 tccggatggc gggccaggtg ctcgcccagg gccgcgctga agccgagcac ggcgagcagc
     1501 cgctcccgca ggccgtcgtc ctcgcgcagc gccgccatca gcgcgtcccg gtcgggggcg
     1561 gcctcggcca ggcggaccag cccgcgcaac gccaggtcgg ggtcggcgct ggccccgagc
     1621 gcctcgacga gccgatctcg gttggcgggc tgcccggcaa gcaccgggtc gacgaggagc
     1681 cgttcggcgg cctgggcgtc ggcgaacccg tgccgggcca gcgctcccag cccggtctgt
     1741 cgccgggcag cgctcacgag ccgatccctt cctcgtgcct cctagagcac cggaaggtag
     1801 cggttgagct cgaagggagt cacctgccgc cggtagtcgg cccactcggc ccgcttgttg
     1861 cgcaacacga agtcgaagac gtgctcaccg agcgtctcgg ccaccagctc gctggcctcc
     1921 atcgcctcga tcgcctcggc caggctggtg ggcaacgggg tgatgcccag gacccggcgc
     1981 tcgcggtcgg tcagcgccca gacgtcgtcc tcggcgccct cgggcagctc gtagccctgc
     2041 tcgacgccct tgagcccggc ggccagcatc accgcgaacg cgaggtaggg gttgcacgcc
     2101 gagtcgggcg agcgcagctc gacccgggtg gactggccct tgtgcggctt gtacatcggc
     2161 acccggatca gcgcgctgcg gttgttgtgc ccccagcaga tgtaggccgg cgcctcgccg
     2221 ccgccggaga gccgcttgta ggagttgacc cactggttgg tcaccgcggt gatctcggcc
     2281 gcgtggtgca gcagcccggc gatgaaggac cggccgacct tggacagctg gtagggcgca
     2341 ccggcctcgt ggaaggcgtt gcggtcgccc tcgaacagcg acaggtgggt gtgcatgccc
     2401 gagccggggt ggtcgctgaa cggcttgggc atgaacgagg cgtagacccc ctgctcgagc
     2461 gcgacctcct tgaccaccag ccggaacgtc atgatgttgt cggcggtcga gagcgcatcg
     2521 gcgtagcgca ggtcgatctc ctgctggccc ggtgcgccct cgtggtggct gaactcgact
     2581 gagatgccca tcgactcgag catggtgatc gccgagcggc ggaagtcgtg cccgatgccg
     2641 tgcggggtgt ggtcgaagta gccgctggag tcgatcggcg ccggcgaccc gtcgggggcc
     2701 ggctccttct cgaacaagaa gaactcgatc tcggggtgcg tgtagaaggt gaagcc
//
LOCUS       DATIBB010000116        25462 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_591394, whole genome shotgun sequence.
ACCESSION   DATIBB010000116 DATIBB010000000
VERSION     DATIBB010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 25462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25462)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25462
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_591394"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..185)
                     /locus_tag="VLC50_04365"
     CDS             complement(<1..185)
                     /locus_tag="VLC50_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019886317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HSF26733.1"
                     /translation="MTASALTSLATGHVGLNVTDLDRSRAFYQAVLGLDVLVEGKDAD
                     HKWVFLARDGQLVLTLW"
     gene            340..873
                     /locus_tag="VLC50_04370"
     CDS             340..873
                     /locus_tag="VLC50_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006143238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABATE domain-containing protein"
                     /protein_id="HSF26734.1"
                     /translation="MQRPLTGEPLALDLVNTVWMRKGVPCDALATVDDLADWLRERGL
                     PAETVAAAATEPVRARLVHTRAVLQAMVDAEPGAEESLNAVLEHGLVVRTLEDQQARE
                     RVVFADPSWELAWRAADDYLRLLGTGPGSIRRCEHPACILWFHDPTGRRRWCSMAGCG
                     NRAKAARHHARTRQSRR"
     gene            complement(916..1302)
                     /locus_tag="VLC50_04375"
     CDS             complement(916..1302)
                     /locus_tag="VLC50_04375"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HSF26735.1"
                     /translation="MVLARVRLTVPGLSEALLELDVLLVDDPAPPAPLLGAPLTAREL
                     SIVRLLPFDLSFRDIGRELFLSLNTVKTHGRSIYRKLGVRSRAEAVVRARELGLFEPD
                     PQIVAIISEEAKPATTSGPVRMPSTA"
     gene            1556..1918
                     /locus_tag="VLC50_04380"
     CDS             1556..1918
                     /locus_tag="VLC50_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017982193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HSF26736.1"
                     /translation="MDLGDVLWAMLAFFFWFMLIWIFIAVIADLFRRDDLSGWGKAGW
                     IVLVVVLPFIGLLIYVVAHPALAQPAARPAGGYNGQHEPSGYSAAEELMKLARLRDDG
                     VISAAEYERMRSRTVMSI"
     gene            1963..3486
                     /locus_tag="VLC50_04385"
     CDS             1963..3486
                     /locus_tag="VLC50_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007238305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HSF26737.1"
                     /translation="MPSPTTQADSAQPRPPTLFDAALPIVVLIGLIATTIVLFGVDAT
                     EGPLQVALLLSAAFASLIAFKNGHAVAAVADAAIGGVTSALGAVFILLAVGALIGAWN
                     MAGTIPTIVYYGIGLLSPTWFYLATTIICALVGMVTGSSWTTAGTLGVAFVGMATVLG
                     LSEAIAAGAVICGAYFGDKMTPLSETTILVPRLVGGITVGQHIRNMFWTSGPALAISL
                     VLFFFIGVRAEPSGAVSTEAAQEILANEYTIAFVNVLPLLLLVGFAIRKVPPFLAILG
                     SALFAAVIAPFTQPAAVKAFVDDSSLGPVATGIKAVFASMASGFVSVTGNEKIDELFS
                     RGGMASMLTTIWLILGALSFAAIAEHAGFLERLLRPIVDRARSRGRLILAVNASGVGL
                     NVIAGDQYVAVVLPSRMFREEFARRRLAPQVLSRAVEDSGTVTSVLVPWNTCGAYISG
                     VLGVSTSAYLPFCFFNMLSPLLDVLFGFIGFKTPSISPADESAEVGRDTPRAGDVAR"
     gene            complement(3508..6285)
                     /locus_tag="VLC50_04390"
     CDS             complement(3508..6285)
                     /locus_tag="VLC50_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HSF26738.1"
                     /translation="MSVTEKPAAAGGAAAPYLRPAADVIEELGTDASAGLSPQEAADR
                     LHTHGANQITAEKPPSALAVALAQLRDPMNLMLVAVAVASLVIGQVPTALLVAALVLL
                     NVVLGARQELKAQASVDALARLQVPQTRVIRGGDVVLVPATDVVPGDVVALEAGDIVP
                     ADGRLLASATLETQEAALTGESAPVSKDAAVLSGPDVSVGDRSNMLFQNTSVTRGTGT
                     IVVTATGMQTQMGQIATMLTSVKRTRSPLQKELDSLTAVLGIIAWSAVALIVVVGLFR
                     DLPIDDVLLLGTAMAISAIPTGLPTFVGAMLSYGAKQLAEAKAVVKNLTDVETLGATS
                     AINTDKTGTLTMNQMMVSTMYLGGSWFTVDGEGYRMSGAVRAAAGTETPDFTNLAYGL
                     VLDSDATVAADGSVVGDPTEAALVVLAGKLGVDAAETRRAYPRLAEVPFDSDYKFMAT
                     FHRVALDGGEHLVELVKGAPDVVLARCSRAGGPLSGSQRPIEQERPAIEAANRQMGEK
                     GLRVLAFAARVLDATDEATLRTDPMSLTRELGFVGLAGIIDPLRAEAKGAVRTALGAG
                     IDVRMITGDHAVTAQAIGDSLGLGPGAISGRELQRMTDDELRHGLTTLHVFGRVAPED
                     KLRLARVMQGEGMVVAMTGDAVNDAAALKQADIGVAMGSGSEVSKQAARMILTDDNFG
                     TLVHAVELGRTIYSKIVSYIRYQMSQLLALVLLFVTATIFDINSGVAMTPLMVLFLNF
                     FISIFPVIVVMVDPGDPDVMRRPPRDPRVPITNRPAVIRWTVYGAVLFLAALVPLVFG
                     PDTLSTDTPSASMTMSFVVVGLGTIFSGLVMRRDPTSGLVPPVLSAVKILVIPAALIV
                     LATELNFLQQGLLTQSLSGPQWLACLGLALILPIVVEVDKWVRRRRSQSPLGFAPELA
                     VNPVRALTEPV"
     gene            complement(6314..7246)
                     /gene="arcC"
                     /locus_tag="VLC50_04395"
     CDS             complement(6314..7246)
                     /gene="arcC"
                     /locus_tag="VLC50_04395"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003246305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HSF26739.1"
                     /translation="MRIVVALGGNALQRRGEPMTAERQRSNVARACAALAPVAQEHEL
                     VITHGNGPQVGLLALQAAAYDELSAYPFDVLGAQTEGMIGYWIEQELGNRLPFEKPLA
                     TILTMTEVDPDDPAFSRPTKFVGPVYPESDANRLASEHGWTVRQDGVGWRRVVASPRP
                     QRIFESRPIEWLLERGAVVICAGGGGIPTAYAKGTRTLVGVEAVIDKDRASAVLAQGL
                     GADLLLIATDVDALYVGWGTEDQHAVSAVHPDALRALDLPAGSMGPKAEAAAEFAAAT
                     GNPCVIGSLDNLGAILAGTGGTRVAVDVDDLVLQ"
     gene            complement(7255..8262)
                     /gene="argF"
                     /locus_tag="VLC50_04400"
     CDS             complement(7255..8262)
                     /gene="argF"
                     /locus_tag="VLC50_04400"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HSF26740.1"
                     /translation="MAVNLRHRDFLKELDFTRTEMQFLLDRSRDLKSAKYAGTEVPRL
                     VGKNIVLIFEKTSTRTRCAFEVSAYDQGAHVTYLDPTSSQLGHKESAADTGRVLSRMF
                     DAIEFRGRAQATVEELAAASDVPVYNGLTDEWHPTQVLADFLTMSEHANGRDPQQITY
                     CFMGDGRFNMGRSLLVMGAIMGSNVRICAPEDLWPDKAVQDLAHERAEESGARITLTA
                     DPDEGLRGADFVHTDVWVSMGEPKEVWASRARDLTPYQVNAAAMARTGNPKAKFMHCL
                     PAFHDTLTTVGREVAEHTGLTGGLEVTDEVFRSPANIAFDQAENRMHTIKAVMVATIG
                     A"
     gene            complement(8332..9561)
                     /locus_tag="VLC50_04405"
     CDS             complement(8332..9561)
                     /locus_tag="VLC50_04405"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014690962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016990 - arginine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HSF26741.1"
                     /translation="MSLHVDSEVGLLRQVIVHRPGLELTRLTPNNVDELLFDDVMWAQ
                     RAREEHDAFVQKLRDKGVKVHLFGELLAQALDQPGAREFVQDELTTATQFGPALDKPL
                     DDLVAAAPADRLSELLIGGVLRSDLKLEEASSLLLKYLDSEDFLLTPLPNHLFQRDNS
                     AWVYDGLSINPMSKPARKRETINSRLVYNFHPMFVKADQPLHFYYGNDSMVHDPATCE
                     GGDILVIGNGTVMIGMGERTTPQGIEFLTRQYFQHGSVTRVIAVELPKSRAFMHLDTA
                     MTMIDRDAFSVYPHLPESLRSFTLTPVGTGGDYQVAENAELFPVVADALGVDKLRVLR
                     TPIDVLGAQREQWDDGNNFLAVAPGVIFGYERNTTTNTFLRKNGIEIVTVAGSELGRG
                     RGGPRCMSCPIERDAVA"
     gene            complement(9589..11079)
                     /locus_tag="VLC50_04410"
     CDS             complement(9589..11079)
                     /locus_tag="VLC50_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020516079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HSF26742.1"
                     /translation="MSTQSAKRSALTGPRTLFASGSRSEPRVMGEAVSDRGPSVEAPR
                     PARGSLGLSQATALIVGSIIGVGIFNLPGSLAAYGPISLLAMALTTIGALALAVMFAA
                     MSRRLPADGGPYAYARTAFGNFAGFSNAWLYWITAWSGNAAIVVGWVLYVQKFVNEGA
                     AKFFSIVLALIGLWVPAAVNLSGVKNMGVVQLWTSVLKFVPLVFMSTVGLFFISTGNF
                     TPWNVSGESSASAIGGAMALCLFSYLGVETAAVAAAKVRDPDRNVPRATIFGTLGTAV
                     VYLLSLVAVFGIVGSTVLADSTAPFSTAVDKIFGGEWAGNLMAVLVIVSGFGALNGWT
                     MICAEMPLAAARDGLFPGRFGKLSDRGVPVFGIVASTVLASVVMICSYAGSAGYTVFN
                     TLVFMSGITAAIPYGFSALAQIKWRRQDNRAIQTPRFVRDVTVAVIALVFSVLFIWYS
                     RNTGESLWKEYLPFIFAGVAFLLGLPIYRSQRAHMTEPAPVPAFRP"
     gene            complement(11141..11229)
                     /locus_tag="VLC50_04415"
     tRNA            complement(11141..11229)
                     /locus_tag="VLC50_04415"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11193..11195),aa:Ser,seq:tga)
     gene            complement(11440..12165)
                     /locus_tag="VLC50_04420"
     CDS             complement(11440..12165)
                     /locus_tag="VLC50_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011758063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF26743.1"
                     /translation="MTTTLDRRRAVPVVVLALVALLAGLLTMALPHHDADAAVATHGS
                     SHGQTVRPANAAQVAFHDDMRKLWEDHVTWTRLAIVTFAADARSGATGKAASFPASAE
                     RLLANQVDIGDAIKPFYGEEAGNTLTALLHDHITIAVEIMQAVVNKDDEAFAHAKARW
                     YANSDDIADFLAAANPKSWPQESMRAAMTTHLDQTLTEAANELGGQFAASVADYDAIH
                     AHILDMADVLSSGIIQAFPEQFR"
     gene            complement(12162..12587)
                     /locus_tag="VLC50_04425"
     CDS             complement(12162..12587)
                     /locus_tag="VLC50_04425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF26744.1"
                     /translation="MHLSYVASRPIVAGPPTVVNVVLRHVVAVAAAISAGVHVALVPD
                     HWAEGGPTLGLAFAVAGVAAGAVALFVRRPEHDVWAPLAAAIVLAATVLAYALSRTVG
                     VPWLISEPEGLDPLGVLTTVAELFGAVSGLLLTTRKDPA"
     gene            12690..12980
                     /locus_tag="VLC50_04430"
     CDS             12690..12980
                     /locus_tag="VLC50_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019044505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF26745.1"
                     /translation="MSAHAIAHLTAATSGGDLGYLDDEVITWRVRRLLDAGFEPGQAR
                     ELAATPGIDLHAVLDLIGRGCPPRLAARILSPGDEPVDEPPHRPGGGRDEPA"
     gene            12977..13654
                     /locus_tag="VLC50_04435"
     CDS             12977..13654
                     /locus_tag="VLC50_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019873488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HSF26746.1"
                     /translation="MTTVGRDALPQAPTPAVGVEDDWVTLLRSPGPDRDAALRRLHQL
                     LLRAARHQVWSMRTTLPARGPEHIEEIANQAADEAMVALLAKLGSFEGRSRFTTWAYK
                     FAVLHAAVEVRRVAWRGREVALLDAAELPEETGSPADYAEAADLARAVRAGIETALTG
                     HQRRIMLTLLVEAVPIDVLAERLGTSRNALYKTLHDARRRLRAHLIDAGYLTVAAPAT
                     TTSRGTR"
     gene            13651..13923
                     /locus_tag="VLC50_04440"
     CDS             13651..13923
                     /locus_tag="VLC50_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013863675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF26747.1"
                     /translation="MSADRRELTPDAVRQLLTDTEPFLSCDDCFDLVDRFVELTLHDP
                     SARLPAMRAHLAGCPACSEEAESLLRLVAADEGIDPDPALRRLHGG"
     gene            13943..14566
                     /locus_tag="VLC50_04445"
     CDS             13943..14566
                     /locus_tag="VLC50_04445"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HSF26748.1"
                     /translation="MDEPGGADRSAYDVIVLAGGTSRRLGGVDKLGLTLAGRPLLDHV
                     LLACRGASRVVAVGPPRPVLADDEVGGRASYELVWCREDPPGGGPLAAVRAGAWHLVS
                     PIVVVVGGDMPYVGPLIGRLVDAVAGGADAAACPDEAGRPLPLPTAFRHEVLAARLSE
                     TSDPSAPPGPLRRLLERVEATLVPANGEDADVDTPEQLAAARERAER"
     gene            complement(14563..14967)
                     /locus_tag="VLC50_04450"
     CDS             complement(14563..14967)
                     /locus_tag="VLC50_04450"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HSF26749.1"
                     /translation="MADELAAATARVRDYLRLCEQRELEAAQAFLSPDAVLVFPGARR
                     YTTVAAMVAEAPSRYRAVAKEILSEDAWRREHGDIGVVTTGVLAGVDVHGRAFAGVRF
                     VDMFVLRDGLIVEQHVWNDLADLGHVPVLLRR"
     gene            complement(14967..16355)
                     /locus_tag="VLC50_04455"
     CDS             complement(14967..16355)
                     /locus_tag="VLC50_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016905957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HSF26750.1"
                     /translation="MSAEDDHDVDVDVVVAGAGAGGLAAALSAAEAGRSVLVVEARES
                     FRHGCNTAMSTAMVPAGGSRWQAAAGIEDSPQRFLDDVRRKTGGRADPDVALALTRVA
                     PTLVGWLADRCGLPLELVTDFDYPGHSRRRCHTVPDRSGATLHRLLLEAVAGDERIDL
                     LVPRRLVDVTTDPVGTVTGVRLQAPGGAGEEISAAAVVLATNGFGANPELVRRHLPGI
                     ADGLYFGGDGSTGDALAVGDRLGADLACLDAYQGHGSVAVPHGVLLTWAAVMHGGVLV
                     NADGARFGDESTGYSEFAGLVLRQPGATAWVVYDERIDSACAAFADYRRCVEAGAIRR
                     ADDVDQLAALVGARSAVLGQTLADASAAAVGAAPDRWGRTHWEAPLAPPYLAVKVTGA
                     LFHTQGGLRVDQQARVLRAGTPVGGLYAVGGAAVGMSGHGADGYLAGNGLLAALGLGY
                     LAGRHVATEGNP"
     gene            complement(16388..17662)
                     /locus_tag="VLC50_04460"
     CDS             complement(16388..17662)
                     /locus_tag="VLC50_04460"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HSF26751.1"
                     /translation="MRVRARAVTALATLVSLSLVLAACGDDDGGSSPTGAATGTSAPT
                     ETLKLGAILTLSGPLAQIGQSHLAGAKLAADQVNKDGGVNGVKFEIVSKDEKADPQAT
                     VQAVRDLQGDDVKLMFGLTTDSDCLAAAPLVSNAGGMLVGTSCQSNLLQGDKFVPGFF
                     EIAPTNYMLSKATARLAADKFPEVQAWDGIGPDYEFGHEVWASFQEQLKALDPGVTFR
                     KNVFVPLTETQFSSHISSLTAGLPADSAEKNGLFMSTFSATTVGLAKQGKSVNLFGRY
                     KVALNLGGSTPTAEALGADTPPLWFIYDYYDGAYDNPTNTKFVADFKAANAGKVPNAW
                     AYEGYTAVMAYRAAIEKAKSTDAKDLIDTMAGMTFDTPKGELTFRKEDHLLQSPVTVW
                     HVQGDAASPGGFKVTEASAIPADQVLPPVPNR"
     gene            complement(17777..18496)
                     /locus_tag="VLC50_04465"
     CDS             complement(17777..18496)
                     /locus_tag="VLC50_04465"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSF26752.1"
                     /translation="MALPDLTVRYLHAYYGTAHVLFAVDLDVPGGATTAVLGRNGAGK
                     TTLLRSIANAGVSTTGQVRYGELELTAMPSYRVARAGVALVPEDRRIFARLSVRDNLA
                     LAAQAAAGTARPALPVGEVVELFPLLGRLLDRPGYALSGGEQQLLAVGRAMVANPTLL
                     LLDEPSEGLAPRVVEQVGEAIRRLRDEFDLSVVLAEQNTRFALELADSVTLIDGGTVV
                     WSGTTAAFASNDAIAHRYLAV"
     gene            complement(18483..19244)
                     /locus_tag="VLC50_04470"
     CDS             complement(18483..19244)
                     /locus_tag="VLC50_04470"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSF26753.1"
                     /translation="MTGAALELAGVTKSFGGNLVLDDVTLTIAPGQVHAVIGPNGAGK
                     STLFGVIAGEHVPERGRVALDGRDITRVPAHRRVGLGVARAFQVAHVFPELTVRENVT
                     SAVIAASGASGTFWRRRPLRVAQPIVDAALDQMRLQDLSERLARELSQGDRKRLEIAM
                     ALELRPGLLMLDEPTAGMSPEETAATVALVRELWERTGLTVLLTEHDMQVVFDLAQQL
                     TVLNLGAVLATGEPSEVRAREDVMDVYLGRSHGAT"
     gene            complement(19241..20260)
                     /locus_tag="VLC50_04475"
     CDS             complement(19241..20260)
                     /locus_tag="VLC50_04475"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HSF26754.1"
                     /translation="MRRRTANLAGLALLVLAVGLLPEVLGTGRLFFWTTAIIAVLFAT
                     TVNLLFGVADIPSFGQAAFFGAGAYTVAMLSPHSWSAPLALLAGIGVAGGVACAASLF
                     TWRLTGLAFAMLTLAIAQGMYTLVVKTDALGGYNGLPGVVAPELGPLNLLDPTVFWYF
                     TAACVALGLAAFWAVGRSPFGHTLRSIRENPSRAIYLGINVRAYRALAFVIAGCGAGL
                     AGGLSAYSNQIVTPGALYWTASATPIIMLLLGGRSYFWGPAIGAVVLSGLLDYLTEAT
                     TAYVFYVGLLLLALLIFLPEGVLSLPRVLRRLARRSRGPRPVDAGVPSVDAAPTDAAA
                     SGGRS"
     gene            complement(20257..21135)
                     /locus_tag="VLC50_04480"
     CDS             complement(20257..21135)
                     /locus_tag="VLC50_04480"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HSF26755.1"
                     /translation="MSILATGLTDGMLIFLVASGLTLILGVMGVVNFAHGGFFVLGAY
                     LAYELLGGVVRSTWVFVLLVLVAALLTAVVGLVSERVLFRRLYRLPEISTLLGTYALL
                     LILQGGVQLIWGQDPRTQPQPAELTQAVGIGSAHVPRYSILVVIAGLLTVVGLQLLIK
                     RSSFGRQLSAVAEDRYMAGLLGINVNRVFAVTFAVGIFLAGLGGGLAGPTLSMVPDIA
                     VTFILQAFAVVIVGGFGSIIGSLIAALVLGLMHAYLVTYAPSLADFSLYLLMAFVLVV
                     RPRGLLGSAAIGQARA"
     gene            complement(21233..22669)
                     /locus_tag="VLC50_04485"
     CDS             complement(21233..22669)
                     /locus_tag="VLC50_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014150797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HSF26756.1"
                     /translation="MGAGREDAVAAAEAVRRGERTPAELVEEAIGRIEAVDDQLGAVI
                     HRFFDRARAEAAEVGAALPAGPFRGVPLLVKDALCHTAGDPYHLGMRLLRDRGWTEPD
                     DTYLAARFRRAGFVIVGKTNTPELATAYTTEPAAYGPTRNPWDTSRTAGGSSGGSAAA
                     VASGMVAIAHGNDMGGSIRVPASACGLVGLKPSRGRTSLGPDFGDYWAMLAHEGVLTR
                     SVRDTAAALDAIAGPAPGDPVSPPAPARPFRAEVGADPGRLRIGLRTRVPVAGTPAHP
                     DCVAAVEVAARTLEACGHRVEEVGMPALDDDELGEHFVDVFAAAIARDLHRWSVRLDQ
                     PIGPDDVEPRNWMLAERGREVDAVRYIASVEHLQAYARRVSQWWTTGFDLLVTPTLPD
                     PPRPLGWLGWEPDLAAVADTGQFTASFNVTGQPAVSLPLHQTDAGLPVGVQLVAAYGR
                     EDLLLRVAAQLEAAAPWAARRPAVWATP"
     gene            22800..23990
                     /locus_tag="VLC50_04490"
     CDS             22800..23990
                     /locus_tag="VLC50_04490"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011757509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HSF26757.1"
                     /translation="MMPLTDLRVVDASTILAGPLCAQILGDFGADVIKVEHPLHGDSM
                     RGHGPQKDGVPLWWKEISRNKRTVGLNLGVPDGAEVFLRLAAGADVVIENFRPGTLER
                     WGLGPDRLLEVNPRLVLVRLTGFGQRGPYAARPGFGTLAEAMSGFAHLTGAADGPPTL
                     PAFGLADSIAGIAASAATLMALRHRDAHGQGQVVDLSILEPIMTAVGPGPTAYDQLGL
                     VENRHGNRSTNNSPRNTYRTRDGSWVAISTSAQTIAERVLRLVGHPEVIDEPWFASGR
                     ARAEHADLLDELVGSWIAARTREEVLAEFEAAGAAVAPIYTARDIVNDPHIRETEMLT
                     QVEDPELGPMLMHNVMWRLSATPGSIRFTGRPQGADTDAVLVKELGLSPDEVARLREA
                     GVVR"
     gene            23987..24832
                     /locus_tag="VLC50_04495"
     CDS             23987..24832
                     /locus_tag="VLC50_04495"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017934715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HSF26758.1"
                     /translation="MTDALLAVVRQGVRVFDLGRPMFAGMPQSPNHPRFTHTLPRRHG
                     DMVRADGGSAANDLLVTGTHVGTHIDALAHVSHDGRLYGGADAAAAGRGGRYLELGVH
                     TIAPMVCRGVLLDVPRALGMPACEAAYEITPADLQATLARQDTNLATGDVVLVRSGWG
                     RRFEEGSEAYRGVSTGVPGVGEAGARWLAEWAPLAVGADTIAFEHLPAGGGHAVLPAH
                     RVLLVEHGVYIVEALALDGLAAAGVHEFTFVLVPLPILGATGSPVRPLAVVAEPSARS
                     AGSDG"
     gene            24825..>25462
                     /locus_tag="VLC50_04500"
     CDS             24825..>25462
                     /locus_tag="VLC50_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013286901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HSF26759.1"
                     /translation="MAEETLARALGRFAAGASFDRLPDAVVTSVRHRVLDILGIAVAA
                     SRLDTSRAARAWALEQGGRPQAHAVGLPDPLPATLAAFVNGVHAHSLDYDDTHLPSVL
                     HPSASVVPAALATAQACRADGRATIAAIAAGLEVCVRVGMAGYDEAAGNSTFFEHGQH
                     ATSICGALGAAVSAAVLLGLDADGVTSSLGVAASMASGILEANRTGGTVKRM"
BASE COUNT         3808 a         9040 c         9013 g         3601 t
ORIGIN      
        1 tgccacagcg tcagcaccag ctggccgtcg cgggcgagga agacccactt gtggtcggcg
       61 tccttgccct cgacgagcac gtcgagcccg agcacggcct ggtagaaggc gcgggaccgg
      121 tcgaggtcgg tgacattgag accgacgtgg ccggtcgcca gcgacgtgag tgcggaagcc
      181 gtcatgatga cctctctcgg ggcgggccgg tcgagcgccg gcggcgccat cttcacgccg
      241 caagccctaa cttgtcaatg ctcgggcaag ggttagatca gcccttagcc gtacgacgat
      301 cttcccggtt tccgcgtgag aaggggttag gctgcgggca tgcaacggcc gctgaccggg
      361 gagcccctcg cgctcgatct ggtcaacacg gtgtggatgc gcaagggcgt cccgtgcgat
      421 gcgctggcca ccgtcgacga cctggccgac tggctgcgtg agcgcgggct gcccgccgag
      481 acggtggccg cggcggcaac cgagccggtc cgggcccggc tcgtgcacac ccgggccgtg
      541 ctgcaggcga tggtcgacgc cgagccgggc gccgaggagt cgctcaacgc ggtgctcgag
      601 cacggcctcg tcgtacgcac gctggaagac cagcaggcgc gggagcgggt ggtgttcgcc
      661 gacccgagct gggagctggc ctggcgcgcg gccgacgact acctgcgact gctcggcacc
      721 ggcccgggca gcatccgccg ctgcgagcac ccggcctgca tcctgtggtt ccacgacccg
      781 accgggcgcc ggcgctggtg ctcgatggcc ggctgcggca accgggccaa ggccgcccgc
      841 caccacgccc gcacgaggca gtcccgacgc tgaggacccg ccggccccgt ccggcgatgc
      901 gtttgcgccc caggcctacg cggtggaggg catcctgacc gggccgctcg ttgtcgccgg
      961 cttcgcctcc tcgctgatga tcgcaacgat ctgagggtct ggctcgaaca ggccgagctc
     1021 tcgggcccgt acgacggcct ccgcgcgcga gcgcaccccg agcttgcgat agatcgaccg
     1081 accgtgggtc ttcaccgtgt tgagcgacaa gaacaattct cgaccgatgt cgcggaatga
     1141 gagatcaaac ggcaacaacc gcacgatcga cagctcccgc gcggtcaacg gagccccgag
     1201 aagcggcgct ggcggggccg gatcatcgac gagcagcacg tccagctcga gaagcgcctc
     1261 gctcagacct gggaccgtga gccgaacccg ggcaagaacc accgcatgcg tgcccgcctc
     1321 agcagagcca ccgcctcgcg gtcggggaat gtgagcctcc atggcgaccg ccctcgcttg
     1381 tgaggcttgc aaccgggcca ttcacaggct agcccggaaa cctcacccgg gtgagacgca
     1441 ccgattcccg cccccatccc gacccggagg gacaacgctt gtcacggcac gggatcggcc
     1501 gtgcaaccgt acgacgtccg gcatcggtcc accgacgcca ggcaaggggg cgacaatgga
     1561 cctcggtgac gttctctggg caatgttggc attcttcttc tggttcatgc tcatctggat
     1621 ttttatcgct gtcatcgccg acttgttccg tcgagatgac ctgtctggat ggggcaaagc
     1681 cgggtggatc gtgctcgtcg tggtgctgcc cttcatcgga ttgttgatct atgtcgtcgc
     1741 gcaccccgcg ctggctcagc ccgccgctcg gcctgccggt ggatacaacg gccagcacga
     1801 gccgagtggc tactccgcag cagaggagct gatgaagctt gcgcgcctgc gagacgacgg
     1861 agtcatcagc gccgccgagt atgaacgcat gagaagccgc acggtgatga gcatctaagg
     1921 gtcgctgccg tccgacctcg gacaaatctc atgtccgacc ccgtgccttc cccgacgacc
     1981 caggctgact cggcccagcc gaggccgccc acgctgttcg acgccgcact gccgatcgtc
     2041 gtgctcatcg ggctcatcgc cacgactatc gtgctgttcg gcgtcgacgc gaccgagggc
     2101 ccgttgcagg tggcgctgct gctcagcgcc gcattcgcca gcctgattgc cttcaagaac
     2161 gggcacgccg tcgctgcggt cgcagacgcg gccatcggtg gggtcacatc ggcgctcggc
     2221 gcagtcttca tcctcctcgc cgtgggcgcg ctcatcggcg cgtggaacat ggccggcacc
     2281 atcccgacaa tcgtctatta cgggatcgga ttgctgagcc cgacctggtt ctatctcgcg
     2341 accaccatca tctgcgccct cgtaggaatg gtcacgggca gctcgtggac cacagccggc
     2401 actctgggcg tcgccttcgt cggcatggcc accgtgctcg ggctctccga ggcgatcgcc
     2461 gccggtgcgg tgatctgcgg cgcctacttc ggcgacaaga tgacgccgct gtcggagacg
     2521 accatcctgg tcccccgcct ggtcgggggg atcacggtcg gccagcacat ccgcaacatg
     2581 ttctggacct ccgggcccgc actggcgatc agcctcgtgc tgttcttctt catcggggtg
     2641 cgcgccgagc ccagcggcgc cgtgagcacc gaagcagccc aggagattct cgccaacgaa
     2701 tacaccatcg cgttcgtcaa tgtgctaccg ctgctgctgc tcgtagggtt cgccatccgc
     2761 aaggtgccgc ccttcctcgc gatcctgggc tccgcattgt tcgctgcggt gatcgcgccg
     2821 tttactcagc ctgccgcagt gaaggcgttc gtcgacgact cgagcctcgg gccggtcgcg
     2881 accgggatca aggccgtctt tgcctcaatg gcctccggct tcgtcagcgt caccggcaac
     2941 gagaagatcg acgagctctt ctcccgcggc gggatggcga gcatgctgac gaccatctgg
     3001 ctcatcctcg gcgcattgtc gttcgcggcc atcgccgagc acgccggatt cctcgagcgg
     3061 ctgctccggc ccatcgtcga ccgggcgaga tcccgcgggc gcctcatcct ggcggtgaac
     3121 gcgagcgggg tcgggctcaa cgtcattgcc ggtgaccagt acgtggctgt cgtgctcccg
     3181 agccggatgt tccgcgagga gttcgccaga aggaggctcg cgccacaggt cctgtcgcgg
     3241 gcggtcgagg actccgggac ggtgacctcg gtactggtgc cctggaacac ctgcggggcc
     3301 tacatctccg gcgtcctggg cgtgtccacc agcgcctatc taccgttctg cttcttcaac
     3361 atgctcagcc cgctgctcga cgtgctgttc ggcttcatcg gattcaagac gccgagcatc
     3421 tctcccgccg acgagtccgc cgaggttggg cgggacacgc cgcgtgccgg cgacgtcgcg
     3481 aggtgaggcg gggagtatcc ggcggcgtca caccggctcg gtgagggcac gcaccgggtt
     3541 cactgcgagc tcgggggcga accccagcgg cgactgagat cggcgtcgac gcacccactt
     3601 gtcgacctcg accacgatcg gaaggatcag ggccaggccc aggcaggcca gccactgtgg
     3661 tcccgacagc gactgggtga gcaggccctg ctggaggaag ttcagctccg tggcgagcac
     3721 gatcagggcg gccgggatga caaggatctt caccgctgag agcaccggcg gcacgagccc
     3781 gctggtcggg tcgcggcgca tgaccaggcc actgaagatc gtcccgagtc ccaccacgac
     3841 gaagctcatg gtcatcgacg cgctgggggt gtccgtgctg agcgtgtccg gcccaaaaac
     3901 cagcgggacg agcgcggcca ggaacagcac cgcgccgtac accgtccagc ggatcacggc
     3961 aggacgattg gtgatgggca ccctcgggtc acgcggcggc cgccgcatca cgtctggatc
     4021 acccggatct accatgacga cgatcaccgg gaagatcgag atgaagaagt tgaggaacag
     4081 caccatgagg ggggtcatgg cgaccccgct gttgatgtca aagatggtgg cggtgacgaa
     4141 gagcagcacc agcgcgagga gctggctcat ctggtaacgg atgtaagaga cgatcttgct
     4201 gtagatcgtc cgtcccagct cgacggcgtg cacgagggtg ccgaagttgt cgtcggtgag
     4261 gatcatgcgc gccgcctgct tggagacctc gctgccgctc cccatggcca cgccgatgtc
     4321 ggcctgcttg agcgccgccg catcgttcac ggcgtcgccg gtcatcgcca caaccatgcc
     4381 ctcgccctgc atgacccggg ccaggcgaag cttgtcttct ggcgcgaccc tgccgaagac
     4441 gtgcagggtc gtcagcccgt gccgcagctc gtcgtcggtc attcgctgca gctcacgccc
     4501 gctgatggcg ccgggaccca gcccgagaga gtccccgatc gcctgcgccg tgaccgcatg
     4561 gtcgccggtg atcatgcgta cgtcgatgcc ggcgcccagc gccgtgcgca ccgccccctt
     4621 ggcctcggcc cgcagggggt cgatgatgcc ggccagcccc acgaagccaa gttcccgagt
     4681 gagcgacata gggtcggtcc gcagcgtggc ctcatcggtc gcgtccagca cccgagccgc
     4741 gaatgccagc acccgcagcc ccttctcgcc catctgacgg ttcgccgcct cgatcgctgg
     4801 acgctcctgc tcgatcgggc gctgcgagcc actgagcgga cccccagcac ggctgcaccg
     4861 ggcgagcacg acgtcgggcg ctcccttcac cagctcgacg aggtgctcgc ctccgtcgag
     4921 ggcaacccgg tgaaaagtcg ccatgaactt gtagtccgag tcgaacggca cctcggccaa
     4981 ccgcggatag gcgcgtctcg tctcggcagc gtcgacgccg agctttcccg cgaggacaac
     5041 cagcgcggcc tcggtcgggt cgccgacgac cgacccgtcc gccgccacgg ttgcgtcgct
     5101 gtccagcacc agaccatagg caagattggt gaaatcggga gtctcggtgc ctgcggcggc
     5161 cctgaccgcc cccgacatcc ggtagccctc cccgtccacg gtgaaccagg acccgccgag
     5221 gtacatcgtc gacaccatca tctggttcat ggtgagcgtg ccggtcttgt cggtgttgat
     5281 ggcgctggtc gcaccgagcg tctccacgtc ggtgaggttc ttcaccacgg ccttggcctc
     5341 ggccagctgc ttcgcgccgt aggagagcat ggcgccgacg aacgtcggga ggccggtcgg
     5401 gatggccgag atcgccatcg cggtgccgag cagcaggacg tcatcgatgg gcaggtcgcg
     5461 gaacagcccg acgaccacga tcagggcgac cgcggaccac gcgatgatcc ccagcaccgc
     5521 ggtgagcgag tcgagctcct tctgcaacgg tgaccgcgtg cgcttgaccg aggtgagcat
     5581 ggtggcgatc tgacccatct gggtctgcat gccggtcgcg gtgacgacga tcgttccggt
     5641 gccacgagtg accgaggtgt tctggaacag catgttggac cggtctccga ccgacacatc
     5701 cggtccggac agcacagccg cgtccttgct caccggggcg ctctcgccgg tcagggccgc
     5761 ctcctgcgtc tccagcgtcg ccgacgcgag cagccggccg tcggccggca cgatgtctcc
     5821 ggcctcgagc gccaccacgt caccagggac cacgtcggtt gcgggcacga ggacgacgtc
     5881 gccgccacgg atcacccggg tctgaggcac ctgcagcctc gccaatgcat ccacgctcgc
     5941 ttgtgccttg agctcctgcc gagcgccgag cacgacgttg aggagcacca gggccgcgac
     6001 gagcaacgcg gtgggcacct ggccgatgac caggctcgcc accgccacgg ccaccagcat
     6061 gagattcatg gggtcccgca gctgtgcgag cgcgaccgcc agcgccgagg ggggtttctc
     6121 ggccgtgatc tggttggccc cgtgggtgtg cagccgatcg gccgcctcct gcggggacag
     6181 ccccgcgctc gcgtcggtgc ccagctcttc gatgacgtcg gccgccggcc gcaggtatgg
     6241 ggctgcggcc ccgccggcgg ctgccggctt ctccgtgacg ctcatcgggg tcctcccctt
     6301 gtgagaagca gcttcactgc aggacgaggt catccacgtc gaccgccacg cgtgttccac
     6361 ccgtgccggc gaggatggcg cccaggttgt cgagcgagcc gatgacgcaa gggttgcccg
     6421 tggccgccgc gaactccgcc gcagcctcgg ccttgggccc catcgaccct gcgggcaggt
     6481 cgagcgcgcg tagggcgtcg gggtgcacgg cggacaccgc gtgctggtcc tcggtgcccc
     6541 acccgacgta gagggcatcc acgtcggtcg cgatgagcaa caggtccgcg ccgaggccct
     6601 gggcgagcac cgcgctggcc cggtccttgt cgatgacggc ttcgacgccg accagggtcc
     6661 gcgtgccctt ggcataggcg gtggggatgc cgccgccacc ggcgcagatc acgacggcgc
     6721 cacgctcaag caaccactcg atcggccggg actcgaagat ccgctggggt cgcggcgagg
     6781 ccaccacgcg acgccagccg acaccgtcct ggcgcaccgt ccacccgtgc tcgctggcca
     6841 gtcggttggc gtccgactcg ggatacaccg gccccacgaa cttcgtcgga cgggagaatg
     6901 ccgggtcgtc ggggtcgacc tcggtcatcg tgaggatggt ggccaggggc ttctcgaagg
     6961 gcagccgatt gcccagctcc tgctcgatcc aatagccgat catgccctcg gtctgcgcgc
     7021 ccagcacgtc gaacgggtag gccgacagct cgtcataggc ggccgcctgc agcgcgagca
     7081 ggccgacctg tggtccgttc ccgtgggtga tgaccagctc gtgctcctgc gccaccggcg
     7141 cgagggccgc gcatgcccga gccacattgg aacgctgtcg ctcggcggtc atgggctcgc
     7201 cacggcgctg cagagcattg cctcccagcg cgacgacgat tcgcatgccg actcctaggc
     7261 accgatggtg gcgaccatga ccgccttgat ggtgtgcatc cggttctcgg cctggtcaaa
     7321 cgcgatgttc gccggtgacc gaaagacctc gtccgtgacc tcgagcccac cggtcaggcc
     7381 ggtgtgctcg gccacctctc gaccgaccgt ggtgagggtg tcgtggaacg cgggcaggca
     7441 gtgcatgaac tttgccttgg ggttcccggt gcgggccatg gcagccgcat tgacctggta
     7501 gggcgtgagg tcgcgcgccc gcgacgccca cacctccttg ggctcgccca tcgacaccca
     7561 gacatcggtg tggacgaagt cggcaccacg caggccctcg tcgggatcgg ccgtgagagt
     7621 gatgcgcgca cccgactcct cggcacgctc gtgggcaagg tcttgcaccg ccttgtcggg
     7681 ccacaggtcc tcaggggcac agatccgcac gttcgagccc atgatcgctc ccatgaccag
     7741 gagcgagcgg cccatgttga accggccgtc acccatgaag cagtaggtga tctgttgtgg
     7801 gtcgcggcca ttcgcgtgct cggacatcgt caggaagtcc gcaagcacct gggtcggatg
     7861 ccactcgtcc gtcagaccgt tgtagaccgg tacgtcggag gcggcggcga gctcctcgac
     7921 cgtggcctgt gcccttccgc ggaactcgat ggcgtcgaac atgcgcgaga gcacccggcc
     7981 ggtgtcggcg gccgactcct tgtgccccag ctgcgacgac gtcgggtcga gataggtgac
     8041 gtgcgccccc tggtcgtagg ccgagacctc gaaggcacac cgggtgcgcg tcgaggtctt
     8101 ctcgaagatc aagacaatgt tcttcccgac caaccggggg acctcggtgc cggcgtactt
     8161 ggcactcttg aggtcccggg accggtcgag caggaactgc atctcggtgc gagtgaagtc
     8221 cagctccttg aggaagtctc ggtgacgcag gttgacggcc atgggaggcg cccctcgtgg
     8281 tgtcgatgac gatgtggtga ctaacggcgg ctcaacaggc ggcgcggttg gtcacgcgac
     8341 cgcgtcgcgt tcgatcgggc aggacatgca gcgcggtcca ccacgacctc gccccaattc
     8401 gctgccggcg acggtcacga tctcgatgcc gttcttgcgc aggaaggtgt tggtcgtggt
     8461 gttgcgttcg tagccaaaga tcactccggg agcgacggcg aggaagttgt tgccgtcgtc
     8521 ccactgctcc cgctgggcgc cgagcacgtc gatcggggtc cggagcactc gcagcttgtc
     8581 cacgcccagg gcatcggcga cgaccgggaa cagctcggcg ttctccgcca cctgatagtc
     8641 cccgcccgtg cccaccgggg tcagcgtgaa cgatcgcagc gactcgggca ggtgcggata
     8701 gacgctgaag gcgtctcggt caatcatcgt catcgcagtg tcgaggtgca tgaaggcccg
     8761 gctcttggga agctccacgg cgatcacccg cgtgaccgag ccatgctgga aatattgacg
     8821 cgtcaggaac tcgatcccct gcggcgtggt ccgctccccc atgccgatca tgactgtgcc
     8881 gttgccgatg acgaggatgt cgccgccctc gcacgtggct gggtcatgca ccatgctgtc
     8941 attgccgtag tagaagtgca gcggctggtc ggctttcacg aacatggggt ggaagttgta
     9001 gacgagccgc gagttgatgg tctcgcgttt gcgtgccggc ttgctcatcg ggttgatcga
     9061 cagtccgtcg tagacccagg cggagttgtc gcgctggaag agatgattgg gcaacggcgt
     9121 aagcaggaag tcctcgctgt cgagatattt gagcaacagg ctcgaggcct cctcgagctt
     9181 caggtcactg cgcaggactc ccccgatcaa cagctcggag aggcgatccg ccggcgccgc
     9241 ggcaacaagg tcgtcgaggg gcttgtccag agccgggccg aactgggtcg cggtggtcag
     9301 ctcgtcctgg acgaactccc gggcccccgg ctggtcgagc gcctgcgcga gcagctcacc
     9361 gaacaggtgc accttgacgc ccttgtccct cagcttctgc acgaaggcgt cgtgttcctc
     9421 acgggcacgt tgcgcccaca tgacgtcgtc aaacagcagc tcgtcaacat tgttaggcgt
     9481 cagccgggtg agctcgagtc cgggccgatg cacgatcacc tgacggagga gtcccacctc
     9541 cgagtcgacg tggagcgaca tgctcctcct cctcccgtgc gcgtcgcact agggacggaa
     9601 tgcgggaacg ggggccggct cggtcatgtg cgcccgctgg gaccggtaga tcggcagacc
     9661 gagcaggaac gcgacccccg caaagatgaa cggcaggtat tccttccaca gggactcgcc
     9721 ggtgttgcgc gagtaccaga tgaacagcac cgagaacacc agcgcgatca ccgctacggt
     9781 gacgtcgcgc acaaatcgcg gggtctggat cgcgcggttg tcctgccgcc gccacttgat
     9841 ctgtgcgagt gcggaaaatc cataagggat ggccgccgtg atgccggaca tgaagacgag
     9901 cgtgttgaag acggtgtagc cggccgagcc ggcgtaggag cagatcatga ccacggaggc
     9961 gagaacagtc gaggccacga tgccgaacac tgggacgccg cggtcggaga gctttccgaa
    10021 gcgcccgggg aacagcccgt ctcgtgcggc ggccagcggc atctccgcgc agatcatggt
    10081 ccagccgttc agtgccccaa atcccgacac gatgaccagc acggccatga ggttgcctgc
    10141 ccactccccg ccgaagatct tgtccacggc ggtcgagaac ggggcggtgg agtcggcaag
    10201 cacggtgctc ccgacgatgc cgaagacggc cacgagggac agcagataga cgacggcggt
    10261 cccgagcgtg ccgaagatcg tcgcgcgcgg aacgttgcgg tccgggtcac gcaccttcgc
    10321 agccgcgacg gcggcggtct cgacgccgag gtaggagaag agacacagcg ccatggctcc
    10381 accgatggca gaggcgctgc tctcaccgct cacgttccag ggggtgaagt tgccggtgct
    10441 gatgaagaac agccccaccg tcgacatgaa caccaacggg acgaacttga gcaccgaggt
    10501 ccacagctgc acgaccccca tgttcttgac accgctgagg ttcacggccg ccggcaccca
    10561 gaggccgatc agagcgagaa cgatcgaaaa gaacttcgcg gcaccttcgt tcacgaactt
    10621 ttgcacgtac aagacccacc cgaccacgat cgcggcattt ccggaccacg ccgtgatcca
    10681 gtagagccag gcgttcgaga agcccgcgaa gttgccgaat gccgtccggg cgtaggcata
    10741 gggaccaccg tcagccggca aacggcgtga cattgccgca aacatcacgg cgagtgccag
    10801 tgcaccgatg gtggtcaggg ccatcgccag caggctgatc ggtccgtaag cggccaaaga
    10861 ccccggcaga ttgaagatcc cgaccccgat gatggatcca acgatcaacg cagtcgcctg
    10921 cgagaggccg agcgacccgc gtgcggggcg gggtgcctcg acgctgggcc ctcggtcgga
    10981 aacggcctcg cccatgaccc gaggttcgct tcgtgaacca gacgcaaaca gggtccttgg
    11041 tcccgtcaac gccgaacgct tcgccgattg ggtcgacaaa gcggcttagg tcgaccacgg
    11101 ccgcgccctg cgccagaacc gcggggccca ctgcgcgtag cggagggcgt gggattcgaa
    11161 cccacgagac cggtcacccg gcctagcggt tttcaagacc gccgcactcg gccactatgc
    11221 gagccctccc gatgcgcagc ccattgtgtc gcatcatcac tcggggcccg tcggcccccg
    11281 gcgggcgccg caccgacagt ggccttacgc accgcagcca ccctcggcgc ccgggatgtc
    11341 cgggtcgcgg ggtggctgcg gtgcgcatcg cgggcggcgg tgtgcacacc gttgaggagg
    11401 ggcggagcag acggcgtgcg ctgagccggc cgcgagcggt cagcggaact gctcggggaa
    11461 ggcctggatg atccctgagc tgagcacgtc ggccatgtcg aggatgtggg cgtggatcgc
    11521 gtcgtagtcg gcgacgctgg ccgcgaactg gccgccgagc tcgttggccg cctcggtgag
    11581 ggtctggtcg aggtgggtcg tcatcgcggc ccgcatggac tcctgcggcc aggacttcgg
    11641 gttggctgcc gcgaggaagt cggcgatgtc gtcggagttg gcgtaccacc gggcctttgc
    11701 gtgggcgaag gcctcatcgt ccttgttcac cacggcctgc atgatctcga ccgcgatcgt
    11761 gatgtggtcg tggagcaggg cggtcagcgt gttgccggcc tcctcgccgt agaacggctt
    11821 gatcgcgtca ccgatgtcga cctggttggc cagcagccgc tccgccgacg ccgggaagct
    11881 ggccgcctta ccggtcgccc cactcctggc gtcggcggcg aacgtgacga tcgcgagccg
    11941 ggtccacgtc acgtggtcct cccacagctt gcgcatgtcg tcgtggaagg cgacctgggc
    12001 ggcgttggcc ggcctgacgg tctgcccgtg cgagctgccg tgcgtggcca ccgcggcgtc
    12061 ggcgtcgtgg tgcggcagcg ccatcgtgag cagcccggcg agcagagcga ccagcgcgag
    12121 caccacaacg ggtacggcgc gtcgacggtc gagggtcgtg gtcatgcggg gtcctttcgg
    12181 gtcgtcagca ggagaccgga cacggcgccg aacagctcgg cgacggtggt gagcaccccc
    12241 agcgggtcga gcccctcggg ctcgctgatc agccacggga ccccgacggt gcgggagagg
    12301 gcgtaggcca gcacggtcgc ggcgagcacg atggcggcgg ccaacggcgc ccacacgtcg
    12361 tgctcgggac ggcgcacaaa cagcgcgacc gccccggcgg ccacccccgc gaccgcgaag
    12421 gccaggccca gcgtcggccc gccctcagcc cagtggtcgg ggaccagcgc gacgtgcacg
    12481 ccggccgaga tcgcggcggc gaccgccacg acgtggcgca gcacgacgtt gaccacggtc
    12541 ggcggcccgg cgacgatggg ccgcgacgcg acgtaagaga ggtgcatgcc gtgttggtgt
    12601 cgatcggggc ggtggtctta ccggaaaggt gcggcgagtt ctcccgcatc ccgggtaaga
    12661 cccagccccg cgaccgacac tcatcagtca tgagcgcaca cgcgatcgcg cacctgaccg
    12721 cagccacctc gggcggcgac ctcggctacc tcgacgacga ggtcatcacc tggcgggtcc
    12781 gccgcctgct cgacgccggc ttcgagcccg ggcaggcccg ggagctggcc gccaccccag
    12841 gcatcgacct gcacgcggtg ctcgacctca tcggccgggg ctgcccgccc aggctggccg
    12901 cgcggatcct ctccccgggg gacgaacccg tcgacgagcc gccccaccgg cctggcggcg
    12961 gccgggacga gccggcatga caaccgtcgg gcgcgacgcc ctgccgcagg caccgacgcc
    13021 cgccgtcggc gtcgaggacg actgggtcac gctgctgcgc tcccccggcc ccgatcgtga
    13081 cgccgcgctg cgccggctgc accagctgct gctgcgcgcc gcccgccacc aggtgtggtc
    13141 gatgcgtacg acgctgccgg cccgcggccc ggagcacatc gaggagatcg ccaaccaggc
    13201 cgccgacgag gcgatggtcg cgctgctggc caagctgggc agcttcgagg ggcgcagccg
    13261 gttcaccacg tgggcctaca agttcgccgt gctccacgcc gcggtcgagg tgcgccgggt
    13321 cgcctggcgc ggccgggagg tcgccctgct cgacgcggcc gagctgcccg aggagaccgg
    13381 ctcgccggcc gactacgccg aggccgccga cctggcccgc gccgtacggg ccgggatcga
    13441 gaccgctctg accggccacc aacggcggat catgctgacc ctgctcgtcg aggcggtgcc
    13501 gatcgacgtg ctggccgagc ggctgggcac ctcgcgcaac gcgctctaca agaccctgca
    13561 cgacgcccgc agacgactgc gggcccatct catcgacgcg ggctacctca ccgtcgcggc
    13621 ccccgccacc accacctcaa gggggacccg atgagcgccg atcgtcgcga gctcacgccc
    13681 gacgcggtgc gccagctcct caccgacacc gagccgttcc tgtcctgtga cgactgcttc
    13741 gacctggtgg accgcttcgt cgagctgacc ctgcacgacc cgagcgcccg gctgccggcg
    13801 atgcgcgccc acctggccgg ctgcccggcc tgctcggagg aggccgagtc gttgctgcgg
    13861 ctggtcgccg ccgacgaggg catcgacccc gacccggcgc tgcgtcggct gcacggcggc
    13921 tgacccgcgc catgctgggg gcatggacga gcccggcggt gccgatcggt cggcgtacga
    13981 cgtcatcgtg ctggccggcg gcacgtcccg ccggctgggc ggcgtcgaca agctcggcct
    14041 gacgctggcc gggcgcccgc tgctcgacca cgtgctcctg gcctgccgcg gcgcgagccg
    14101 ggtcgtcgcg gtcggtccgc cccgaccggt gctggccgac gacgaggtcg gcggccgggc
    14161 gtcctatgag ctggtgtggt gccgggagga cccccccggc ggggggccgc tggccgcggt
    14221 cagggcgggg gcgtggcacc tcgtcagccc gatcgtcgtg gtggtcggcg gcgacatgcc
    14281 atacgtcgga ccgctgatcg gccggctggt cgacgcggtg gccggcggcg ccgacgccgc
    14341 ggcctgcccc gacgaggccg ggcgcccgct gccgctgccg accgcgttcc ggcacgaggt
    14401 gctcgcggca cggttgtccg agaccagcga cccgtccgcg ccgcccgggc cgctgcgccg
    14461 gctgctcgag cgggtcgagg cgacgctggt gccggccaac ggcgaggacg ccgacgtcga
    14521 cacccccgag cagctggcgg ccgcgcgcga gcgggccgag cgctagcgcc tcagcagcac
    14581 cggcacgtgg cccaggtcgg ccaggtcgtt ccacacgtgc tgctcgacga tcaggccgtc
    14641 gcgcagcacg aacatgtcga cgaagcgcac ccccgcgaag gcgcgcccgt gcacgtcgac
    14701 gccagcgagg acgccggtcg tcaccacccc gatgtcaccg tgctcgcggc gccacgcgtc
    14761 ctccgagagg atctccttcg ccaccgcccg gtagcgcgac ggcgcctccg cgaccatcgc
    14821 cgccacggtg gtgtagcggc gcgcaccggg gaagaccagc accgcatccg ggctcaggaa
    14881 ggcctgcgcc gcctcgagct cacgctgctc gcacagccgc aggtagtcac gcacgcgagc
    14941 ggtcgccgcg gcaagctcgt cagccatcag gggttccctt ccgtcgcgac gtgtcgcccg
    15001 gcaaggtagc caaggcctag cgcggccaac agcccgttgc cggccaggta gccgtcggca
    15061 ccgtgcccgg acatccccac cgcggcaccg cccacggcgt agagcccgcc gaccggtgtc
    15121 ccggcccgca gcacccgggc ctgctggtcc acccgcagcc cgccctgggt gtggaacagc
    15181 gccccggtca ccttgaccgc gaggtacggc ggggccagcg gggcctccca gtgcgtccgg
    15241 ccccaccggt cgggcgccgc gccgacggcg gccgccgagg cgtcggccag ggtctgcccg
    15301 aggacggccg accgggcgcc gaccagcgcg gctagctggt cgacgtcgtc ggcccgccgg
    15361 atggcgccgg cctcgacaca gcggcggtag tcggcaaacg cggcgcaggc actgtcgatg
    15421 cgctcgtcgt agaccaccca ggcggtcgcg ccgggctggc gcagcaccaa cccggcgaac
    15481 tcggagtagc ccgtcgactc gtcgccgaag cgggccccgt cggcgttgac gaggacgccg
    15541 ccgtgcatga cggccgccca ggtcagcagc accccgtgcg gcaccgccac cgagccgtgg
    15601 ccctggtagg cgtccaggca cgccaggtcg gcgccgagcc ggtcgccgac ggccagcgcg
    15661 tcaccggtgc tgccgtcgcc accgaagtag agcccgtcgg cgatcccggg caggtgccga
    15721 cggaccagct cggggttggc gccgaaaccg ttggtggcca gcaccaccgc ggccgccgag
    15781 atctcttcgc ccgcaccgcc cggcgcctgc aggcggacgc cggtgaccgt gccgaccggg
    15841 tcggtggtca cgtcgaccag ccggcgcggc accagcaggt cgatgcgctc gtcgccagcc
    15901 accgcctcga gcagcagtcg gtgcagggtc gccccggaac ggtcgggcac cgtgtggcag
    15961 cgccgccggg agtggcccgg gtagtcgaag tcggtgacca gctcgagcgg gagcccgcac
    16021 cgatcggcca gccagccgac cagcgtcggc gcgacccgag tgagggccag cgcgacgtcc
    16081 gggtcggccc ggccgccggt cttgcgccgt acgtcgtcaa ggaaccgctg cggcgagtcc
    16141 tcgatgccgg ccgccgcctg ccaccggctg ccgccggcgg gcaccatcgc ggtgctcatc
    16201 gcggtgttgc agccgtgccg gaagctctcc cgcgcctcga cgaccagcac gctgcgccct
    16261 gcctcggcgg cggacagcgc ggcggccagc ccacccgcac cggcgccggc caccacgacg
    16321 tcgacgtcga cgtcgtggtc gtcctcggcg ctcaccgttt gcgcttgatc agtgcagggg
    16381 tgggttgtca gcggttgggg accgggggca gcacctggtc ggccgggatc gccgaggcct
    16441 cggtcacctt gaacccgccc ggggaggcgg cgtcgccctg gacgtgccag acggtgaccg
    16501 gcgactggag caggtggtcc tccttgcgga aggtcagctc tcccttgggg gtgtcgaagg
    16561 tcatgcccgc catcgtgtcg atgaggtcct tggcgtcggt gctcttggcc ttctcgatcg
    16621 cggcccgata ggccatcacg gcggtgtagc cctcgtaggc ccaggcgttg ggcaccttgc
    16681 cggcgttggc ggccttgaag tcggcgacga acttggtgtt ggtcgggttg tcgtaggcgc
    16741 cgtcgtagta gtcgtagatg aaccagagcg gcggggtgtc cgcgcccagc gcctcggcgg
    16801 tgggcgtcga gccaccaagg ttgagcgcca ccttgtagcg gccgaacagg ttgaccgact
    16861 tgccctgctt ggccagcccg acggtggtgg ccgagaacgt cgacatgaac aggccgttct
    16921 tctcggcgct gtcggcgggc aggcccgcgg tcagcgagct gatgtgcgag ctgaactgcg
    16981 tctcggtcag cggcacgaac acgttcttgc ggaacgtcac gcccgggtcg agcgccttga
    17041 gctgctcctg gaaggacgcc cagacctcgt ggccgaactc gtagtcgggc ccgatgccgt
    17101 cccaggcctg cacctccggg aacttgtcgg cggccagccg cgcggtcgcc ttggacaaca
    17161 tgtagttggt cggggcgatc tcgaagaagc cgggcacgaa cttgtcgccc tgcagcaggt
    17221 tggactggca cgaggtgccg accagcatgc cgccggcgtt ggagaccagc ggcgccgcgg
    17281 ccaggcagtc ggagtcggtg gtcaggccga acatcagctt gacgtcgtca ccctgcaggt
    17341 cccgcaccgc ttgcacggtg gcctgcgggt cggccttctc gtccttggag acgatctcga
    17401 acttcacgcc gttgacgccg ccgtccttgt tgacctggtc agcggcgagc ttggccccgg
    17461 ccaggtggct ctggccgatc tgggccagcg ggccgctcag cgtgaggatc gcgcccagct
    17521 tgagggtctc cgtgggcgcg ctggtgccgg tggcggcacc ggtcggcgac gaccctccgt
    17581 cgtcgtcgcc gcacgcggcg aggacgagcg agagagagac cagcgtggcg agggcggtga
    17641 cggcacgggc acgaactctc atcgggggcc tcctggtaac cgaccggttg gttcgagaat
    17701 gttccactta acggaacgcc gtttcacgag ttggctgatt cctaccatgc gcgccgggat
    17761 cgcggtagcg aaggcgtcag accgcgaggt atcgatgcgc aatcgcgtcg ttgctggcga
    17821 aggcggccgt cgtcccggac cacaccaccg tgccgccgtc gatgagcgtc accgagtcgg
    17881 ccagctcgag ggcgaagcgg gtgttctgct cggccagcac gaccgacagg tcgaactcgt
    17941 cgcgcagccg ccggatcgcc tcgcccacct gctcgaccac ccggggcgcc agcccctcgg
    18001 acggctcgtc gagcagcagc agggtgggat tggcgaccat cgcccggccg acggcgagca
    18061 gctgctgctc cccgccgctg agcgcatagc cgggccggtc gagcagccgg cccagcagcg
    18121 ggaacagctc gacgacctcg ccgaccggca gcgcgggccg agccgtcccg gccgcggcct
    18181 gcgcggccag cgccaggttg tcgcgcaccg agagccgggc gaagatccgc cggtcctccg
    18241 ggaccagggc caccccggcc cgggccaccc ggtaggacgg catcgcggtc agctcgagct
    18301 cgccatagcg gacctgtccc gtggtgctca ccccggcgtt ggcgatgctg cgcagcagcg
    18361 tcgtcttgcc ggccccgttg cggccgagca ccgccgtggt ggcgccgccg ggcacgtcga
    18421 ggtcgaccgc gaagaggacg tgcgcggtgc cgtagtaggc atgcaggtag cgcacggtga
    18481 ggtcaggtag cgccatggct cctccccagg tagacgtcca tgacgtcctc gcgggcccgg
    18541 acctctgagg gctcgccggt ggccagcacg gcgccgaggt tgagcacggt gagctgctgg
    18601 gccaggtcga agaccacctg catgtcgtgc tcggtcagca gcacggtcag gccggtgcgc
    18661 tcccacagct cgcgcaccag cgcgacggtc gcggcggtct cctcgggcga catgccggcg
    18721 gtcggctcgt cgagcatgag caggccgggg cgcagctcca acgccatcgc gatctcgagg
    18781 cgtttgcggt cgccctgcga cagctcgcgg gccagccgct cggacaggtc ctgcaggcgc
    18841 atctggtcga gcgccgcgtc cacgatcggc tgggccaccc gcagcggccg gcggcgccag
    18901 aacgtgcccg aggcgccgga ggcggcgatc accgccgagg tgacgttctc gcgcacggtc
    18961 agctcgggga acacgtgggc cacctggaag gcccgcgcga ccccgagccc gacccggcgg
    19021 tgggccggca cccgggtgat gtcgcggccg tcgagcgcga cgcggccccg ctcggggacg
    19081 tgctcgccgg cgatgacccc gaacagggtg ctcttgcccg cgccgttggg gccgatgacg
    19141 gcgtgcacct ggccgggggc gatggtcagc gtcacgtcgt cgagcacgag gttgccgccg
    19201 aaggacttgg tcaccccggc cagctccagg gccgccccgg tcatgaccga cctccgctgg
    19261 cggccgcgtc ggtcggggcc gcgtcgaccg acggcacgcc ggcgtcgacc ggccgggggc
    19321 cgcggctccg gcgggccagc cggcgcagca cccgcggcag gctcagcacg ccctcgggca
    19381 ggaagatcag cagggcgagc agcagcaggc cgacgtagaa cacgtaggcg gtggtggcct
    19441 cggtcaggta gtcgagcagc ccggacagca ccaccgcacc gatggctggc ccccagaagt
    19501 aggaccgccc gccgagcagc agcatgatga tcggtgtcgc cgaggcggtc cagtagagcg
    19561 cccccggggt gacgatctgg ttggagtagg cggacagccc gccggccagc ccggcgccgc
    19621 agccggcgat gacgaaggcc agcgcccggt aggcgcgcac gttgatgccg aggtagatgg
    19681 cgcgcgaggg gttctcccgg atcgagcgca gcgtgtgccc gaacggcgag cggcccacgg
    19741 cccagaacgc ggccagcccc agcgcgacgc aggccgccgt gaagtaccag aacaccgtgg
    19801 ggtccaagag gttgagcggg cctagctccg gcgccaccac gcccggcagc ccgttgtagc
    19861 cgcccagcgc gtcggtcttg accacgagcg tgtacatccc ctgcgcgatg gcgagcgtga
    19921 gcatcgcgaa ggccagcccg gtcagccgcc aggtgaacag cgaggcggcg cacgccaccc
    19981 cgccggccac cccgatgccg gcgagcagcg ccagcggggc cgaccagctg tgcggcgaga
    20041 gcatggcgac cgtgtaggcg ccggcgccga agaacgcggc ctggccgaac gacgggatgt
    20101 cggcgacgcc gaagagcagg ttgaccgtgg tggcgaagag caccgcgatg atcgcggtgg
    20161 tccagaagaa cagccggccg gtgccgagca cctcgggcag cagcccgacg gccagcacga
    20221 gcagggccag cccggccagg ttcgccgtac ggcgcctcat gcccgcgcct gtccgatcgc
    20281 ggcggacccg agcagcccgc gggggcggac gaccagcacg aaggccatca gcaggtagag
    20341 ggagaagtcg gccagcgacg gcgcataggt gaccaggtag gcgtgcatca gccccaggac
    20401 cagcgcggcg atcagcgagc cgatgatgct gccgaagccg ccgacgatga ccaccgcgaa
    20461 ggcctgcagg atgaacgtca ccgcgatgtc ggggaccatg ctcagagtcg gcccggccag
    20521 cccgccgccc agcccggcca ggaagatccc gacggcgaag gtcaccgcga acacccggtt
    20581 gacgttgatg cccagcagcc cggccatgta gcggtcctcg gccaccgcgc tgagctggcg
    20641 gccgaacgag ctgcgcttga tcagcagctg cagcccgacc acggtgagca gcccggcgat
    20701 gacgaccagg atgctgtagc ggggcacgtg ggcgctgccg atgccgaccg cctgggtcag
    20761 ctcggcgggc tgcggctggg tgcgcgggtc ctgcccccag atcagctgga cgccgccctg
    20821 caggatcagc agcagcgcgt aggtgccgag cagcgtgctg atctcgggca gccggtagag
    20881 ccggcgaaac agcacccgct cgctgaccag cccgaccacg gcggtgagca gcgccgcgac
    20941 gagcaccagc aggacgaaga cccaggtgga gcgcaccacg ccgccgagca gctcgtaggc
    21001 gagataggca ccgagcacga agaacccacc gtgggcgaag ttgaccacgc ccatcacgcc
    21061 gaggatcagg gtcagcccgc tggcgaccaa gaagatcagc atgccgtcgg tgagcccggt
    21121 ggcgaggatg ctcacgacgg ccccgacccg gtcatgttca cgctttcgcc ccacatgttc
    21181 cgattatcgg tacgccgttc cgcgttcacg ctgtcagagg ggcgcagggg tgtcaaggag
    21241 tggcccagac cgccgggcgc cgcgccgccc acggggcggc ggcctcgagc tgggcggcca
    21301 cccgcagcag caggtcttcc cggccgtagg ccgccacgag ctgcacgccg actggcagcc
    21361 cggcatcggt ctggtgcagc ggcagcgaca ccgccggctg gccggtgacg ttgaacgacg
    21421 cggtgaactg cccggtgtcg gcgaccgcgg ccaggtctgg ctcccaaccc agccagccca
    21481 gcggccgggg cgggtcgggc aacgtgggcg tgacgagcag gtcgaagccg gtggtccacc
    21541 actggctgac ccggcgggcg taggcctgca ggtgctcgac cgaggcgatg tagcgcaccg
    21601 cgtcgacctc ccggccgcgc tcggcgagca tccagttgcg cggctcgacg tcgtcggggc
    21661 cgatcggctg gtccagccgg acgctccagc ggtgcaggtc gcgggcgatc gcggccgcaa
    21721 agacgtcgac gaagtgctcg ccgagctcgt cgtcgtcgag cgccggcatc cccacctcct
    21781 ccacccggtg cccgcaggcc tcgagcgtgc gggcagccac ctcgacggct gcgacgcagt
    21841 cggggtgggc cggtgtgccg gcgaccggga cccgggtgcg cagcccgatg cgcagccggc
    21901 cggggtcggc gcccacctcg gcgcggaacg gccgggccgg cgccggcggg ctcaccgggt
    21961 cgccgggggc cgggccggcg atcgcgtcga gggccgccgc cgtgtcgcgc accgaccggg
    22021 tgagcacccc ctcgtgggcc agcatcgccc agtagtcgcc gaagtcgggg cccagcgagg
    22081 tgcgcccccg gctcggcttg agcccgacca gcccgcaggc gctggccggc acccggatcg
    22141 acccgcccat gtcgttgccg tgcgcgatcg cgaccatccc cgaggcgacc gccgcggccg
    22201 agccgccgct ggacccgccg gcggtgcgcg aggtgtccca cgggttgcgg gtcggcccgt
    22261 aggccgccgg ctcggtggtg taggcggtgg ccagctcggg cgtgttggtc ttgccgacga
    22321 tgacgaaccc ggcccggcga aaccgcgccg ccaggtaggt gtcgtcgggc tcggtccagc
    22381 cgcggtcgcg cagcaggcgc atgccgaggt ggtaggggtc accggcggtg tggcacagcg
    22441 cgtccttgac cagcagcggg accccccgga acggcccggc gggcagcgcg gcaccgacct
    22501 cggcggcctc ggcccgggcc cggtcgaaga accgatggat caccgcgccg agctggtcgt
    22561 cgaccgcctc gatgcgcccg atcgcctcct cgaccagctc ggccggggtg cgctcaccgc
    22621 gccgtaccgc ctcggccgcc gccaccgcgt cctcgcgtcc cgcacccaca gcccgcgctc
    22681 cctccgcgcc gtcgccctcg cccggacctc gtcgcgaccc tggcttgggc ccgacctcgt
    22741 ggctatagtg ccctgaaaca ccgtttcgcg ctgcggaacg atggagggga tggccgccga
    22801 tgatgccgct gacggatctg cgcgtggtcg acgcgtccac gatcctggcc ggcccgctgt
    22861 gcgcccagat cctgggcgac ttcggcgccg acgtcatcaa ggtcgagcac ccgctccacg
    22921 gcgacagcat gcgtgggcac ggcccgcaga aggacggcgt gccgctctgg tggaaggaga
    22981 tcagccgcaa caagcgcacc gtcgggctca acctcggggt gcccgacggc gccgaggtgt
    23041 tcctgcggct ggccgccggc gccgacgtgg tgatcgagaa cttccgcccc ggcaccctgg
    23101 agcgctgggg gctggggccc gaccggctgc tcgaggtcaa ccccaggctg gtgctggtgc
    23161 ggctgaccgg cttcggccag cgcgggccct acgcggcccg gcccggcttc ggcacgctcg
    23221 ccgaggcgat gagcgggttc gcccacctga ccggggcggc ggacgggccg ccgacgctgc
    23281 cggcgttcgg gctggccgac agcatcgccg gcatcgcggc gtcggcggcc accctgatgg
    23341 cgctgcgcca ccgcgacgcc cacgggcagg gccaggtggt cgacctgagc atcctcgagc
    23401 cgatcatgac cgcggtcggc cccggtccca ccgcctatga ccagctcggg ctggtcgaga
    23461 accggcacgg caaccggtcg accaacaact ctccccgcaa cacctaccgc acccgcgacg
    23521 ggtcctgggt cgcgatctcc accagcgcgc agaccatcgc cgagcgggtg ctgcgcctcg
    23581 tcgggcaccc ggaggtgatt gacgagccct ggttcgccag cgggcgggcc cgggccgagc
    23641 acgccgacct gctcgacgag ctcgtcgggt cgtggatcgc ggcccgcacc cgcgaagagg
    23701 tgctggccga gttcgaggcc gccggcgcgg cggtcgcgcc gatctacacc gcccgcgaca
    23761 tcgtcaacga cccgcacatc cgcgagaccg agatgctcac gcaggtcgag gacccagagc
    23821 tggggccgat gctgatgcac aacgtgatgt ggcgcctgtc ggcgaccccc gggtcgatcc
    23881 ggttcaccgg ccggccgcag ggcgcagaca ccgacgccgt actggtcaag gagctggggc
    23941 tctcgcccga cgaggtggcg cggctgcgcg aggccggggt ggtccgatga ccgacgccct
    24001 gctcgccgtc gtacgccagg gggtgcgggt ctttgacctg ggccggccga tgttcgccgg
    24061 catgccgcag tcgcccaacc acccgcgttt cacccacacg ctgccccgcc ggcacggcga
    24121 catggtgcgc gcggacggcg gctccgcggc caacgacctg ctcgtcaccg gtacccatgt
    24181 gggcacgcac atcgacgcgc tcgcgcacgt ctcgcacgac ggtcggctgt acggcggtgc
    24241 cgacgcggcc gccgccggcc ggggcgggcg ctacctcgag ctgggggtgc acacgatcgc
    24301 gccgatggtc tgccgcgggg tgctgctcga cgtgccgcgg gcactgggga tgccggcgtg
    24361 cgaggcggcc tacgagatca ccccggctga cctgcaggcg accctggcgc gccaggacac
    24421 caacctcgcc accggcgacg tcgtgctggt gcgcagcggc tggggccggc gcttcgagga
    24481 gggcagcgag gcctaccgcg gcgtgagcac cggcgtgccc ggggtgggcg aggccggggc
    24541 gcgctggctg gccgagtggg cccccctcgc cgtgggcgcc gacacgatcg cgttcgagca
    24601 cctgccggcc ggcggcgggc acgccgtgct gccggcgcac cgggtgctgc tcgtcgagca
    24661 cggcgtctac atcgtcgagg cgctggccct cgacgggctc gccgccgctg gggtgcacga
    24721 gttcacgttc gtcctggtgc cgctgcccat cctcggcgcc accgggtcgc cggtccgccc
    24781 gctcgccgtg gtggccgagc cctccgcccg ctcggccggt tccgatggct gaagagacgc
    24841 tggcccgggc gctggggcgg ttcgctgccg gggccagctt tgaccggctg cccgacgcgg
    24901 tggtcaccag cgtgcgccac cgggtcctcg acatcctggg catcgccgtg gccgccagcc
    24961 ggctcgacac cagccgggcc gcgcgcgcct gggcgctcga gcagggcggc cggccgcagg
    25021 cccacgcggt gggtctgccc gacccgctcc ccgcgacgct ggccgccttc gtcaacggcg
    25081 tgcacgcgca ctccctcgac tacgacgaca cccacctgcc ctccgtgttg cacccgagcg
    25141 cctcggtcgt cccggccgcg ctggcgacgg ctcaggcctg ccgggccgac ggccgggcca
    25201 ccatcgcggc gatcgccgcc ggcctcgagg tgtgcgtgcg ggtcgggatg gccggctacg
    25261 acgaggccgc cggcaactcc acgttcttcg agcacggtca gcacgccacc tcgatctgcg
    25321 gggcgctcgg ggccgccgtc tcggccgccg tgctgctcgg cctggacgcc gacggggtga
    25381 ccagctcgct cggggtggcc gcctcgatgg cctcgggcat cctcgaggcc aaccgcaccg
    25441 gcggcaccgt caagcggatg ca
//
LOCUS       DATIBB010000117         8087 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_460412, whole genome shotgun sequence.
ACCESSION   DATIBB010000117 DATIBB010000000
VERSION     DATIBB010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 8087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8087
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_460412"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(56..334)
                     /locus_tag="VLC50_04505"
     CDS             complement(56..334)
                     /locus_tag="VLC50_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011754979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HSF26760.1"
                     /translation="MTLTVLADNRMRVTDRVPCRVFGPDLFFSESPSEVELAKSLCHE
                     CPLRSECLEGALERREPWGVWGGELFAAGVVVPRKRPRGRPRKTEAAA"
     gene            complement(464..670)
                     /locus_tag="VLC50_04510"
     CDS             complement(464..670)
                     /locus_tag="VLC50_04510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF26761.1"
                     /translation="MSTTTLVNGVGAAATYGMGGFASPTAGDRIAVNLAHRDRLLVAG
                     GFGAAIAVITEISSRAPRAWSPLV"
     gene            complement(844..2952)
                     /locus_tag="VLC50_04515"
     CDS             complement(844..2952)
                     /locus_tag="VLC50_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005312262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase UvrD2"
                     /protein_id="HSF26762.1"
                     /translation="MSTISPDLAEPAGADAVLAGLDPEQRAVATALTGPVCVLAGAGT
                     GKTRAITHRIAYGVLAGVYAPQHVLAVTFTNRAAGELRGRLRALGVPGVQARTFHSAA
                     LRQLQYFWPRTIGGTPPRVIAAKVSLIAEAAGRLGLRPHRTDLRDLAAEVEWAAVSLV
                     PRDGYVAALARAGRTPPAGLDATTVERLLGGYDEIKRERDVMDFEDVLLITVGLLAEH
                     AEVAEAVRAQYRHFVVDEYQDVSPLQQQLLEQWLGGRDSLCVVGDPGQTIYSFAGATP
                     SYLLGFRTRHPAATVVRLVRDYRSTPQVVGLANGVLKAGLPAGDGVELRAVRESGPAP
                     TFCEYADEPAEAHGVAERIRALVAAGVPAHEIAVLFRTNGQSAAYEEALTEAGVPFLL
                     RGGERFFERREVREAHLLLRAAARSGRTEEPLVDVVRAVLGGMGWSTTPPQGAGAARD
                     RWESLAALVGLAENLLARQPAAGLAELVDELDDRAATAHAPVVDAVTLASLHAAKGLE
                     WDAVFLVGLTEGMVPISYAQTPEAVAEECRLLYVGVTRAREHLALSWTRSRAPGGAPT
                     RRPSRFLDGLRPADQRDVERAGAGRTRTGRRRPPAVCRVCGRPLSSAVARKLGRCVDC
                     PVDIDEATFERLRAWRLERARVASVPAFVVFTDATLTAIAELRPTTLEALATVPGVGR
                     TKLDRYGAEVLDLVGETSSG"
     gene            3066..3314
                     /locus_tag="VLC50_04520"
     CDS             3066..3314
                     /locus_tag="VLC50_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004937444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mycoredoxin"
                     /protein_id="HSF26763.1"
                     /translation="MTANAEITMYSTTWCGYCRRLKSQMDREGIAYREVNIELDPRAA
                     DFVMSVNGGNQTVPTVLFPDGSALTNPSLAEVKGKLAA"
     gene            complement(3321..4253)
                     /gene="nudC"
                     /locus_tag="VLC50_04525"
     CDS             complement(3321..4253)
                     /gene="nudC"
                     /locus_tag="VLC50_04525"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012894738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HSF26764.1"
                     /translation="MSSSAGLTQLALSRASVDRAAHHRTDQAWLDARWADPATRVLVL
                     EHGQARVVHDGAAPTLLLVAPDQAPDGLQILLGVDADDVACFAVVTDPGRNPDLGEAN
                     GTRWVGLREVGALLTDTEAGLFVHAAAMANWHETHSHCPRCGAPTEPVLSGSVRRCPA
                     DGSEHYPRTDPAVIMTVVDDDDRLLLGHQARWPNGRFSTLAGFVEPGESLEAAVRREV
                     LEEVGLHVGVVTYMGSQPWPFPSSLMLGFHAHAIDCEAHPDGHEISDARWFTRPELAA
                     AVAARDVLLPSPVSIARRLVENWYGGPVGEDLAW"
     gene            4340..5062
                     /gene="deoD"
                     /locus_tag="VLC50_04530"
     CDS             4340..5062
                     /gene="deoD"
                     /locus_tag="VLC50_04530"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011754975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0043096 - purine nucleobase salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HSF26765.1"
                     /translation="MSTHIGAAADEISEHVLLPGDPLRAKWIAENFLTDAHCYSSVRN
                     MLGFTGTYRGERISVQGTGMGQPSLSIYVHELCTEYGARTLVRVGSCGALAPDLSLRD
                     VVLAMSASTDSAINHVRFEGIDFAPTADFELLRAAHDAARARGLDVHVGPVASWDVFY
                     SDRPELLRRLAEYGVLAVEMETAALYTLAAKYHARGLALLTVSDSLVSGEETSAQERE
                     QTFGEMVELALDSVLSLAKTDG"
     gene            complement(5068..5694)
                     /locus_tag="VLC50_04535"
     CDS             complement(5068..5694)
                     /locus_tag="VLC50_04535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF26766.1"
                     /translation="MLVTIGGGLTIASSAFGPGPAMLVAAGLVASAWWASRVARRITN
                     RRLVMVTTLSPSWRVQLTAAAHARRRALIAAARMPDGLGDGAELDSAVTTALWQAVTL
                     LLEADDLRRELASAGIDGADNDEAHQAISQAISQAGELLASITRAADASEDLARAADG
                     RQLLLADRAKELRLATFRAGRDLTVDELAERRRALAVALRRLQHGEEP"
     gene            complement(5870..>8087)
                     /locus_tag="VLC50_04540"
     CDS             complement(5870..>8087)
                     /locus_tag="VLC50_04540"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HSF26767.1"
                     /translation="GSGRVVAAIHETVEDEAAAIADALARVWRADDPGAAAGRARRTV
                     AVLCRKRSQFPAVEAALRARGLPVEVVGLGGLLSTPEVADLVATLQVVHDPTRGDALM
                     RLLTGPRWRLGPRDLEGLGRWSRRLARARAAAGAGGREPVEGPAEAGDTPVPDAVDER
                     SIVEALDELPRRPWRDADGVGLSEPALRRLASLAAELRGLRARVGASLPEIVVDVERT
                     IGIDIEVAARPGRSAAAARAHLDAFLDVAAGFADSAQTASLGGFLAWLQAAELRERGL
                     QPGEVDVTDQAVQVLTVHAAKGLEWDVVAVPGLVEGIFPACGDVDGGWLSGLGPLPFP
                     LRGDAAGLPVWRWDRCASQEELKEQWQSFRGSAGSHELAEERRLAYVAVTRARSLLLL
                     TGHRWDDAGAPREVSRFLAEMVEPTTAQLAGVVERGTWVPRPDGTNPRTASAATHPWP
                     LDPLGERRAVVEAGAALVQMALTAAAHDEGDQTDPADPTDPADPTDPPAVACLTETTE
                     PDDPWSAEVDLLLAERARARREVIEVALPAHLSTSRLVRLAEDPDALARDIRRPVPGP
                     PRPAARRGTAFHTWLEQRFGQAAMVDLFDLPGAADESATGDEDLAALKATFLASEWAD
                     RLPLEVEVAFETTIGPVLLRGRADAVFATADSGFEVVDWKTGRPPTGDAARVRAVQLA
                     VYRLAWSRLRAVPLERVSAAFFYAATGQTVRPVDLFDEADLLALVDALPEVGSIRG"
BASE COUNT         1183 a         3007 c         2901 g          996 t
ORIGIN      
        1 gttgtgagta cgacgccagg tgcgggcccg gcgtgagaac ggaaggcggg cccgctcatg
       61 cggccgcctc ggtcttgcgg ggacggcccc gcgggcgctt gcgtggcacg acgactccag
      121 ccgcgaacag ctcgccgccc cagacgcccc acggctcacg ccgctcgagt gcaccctcca
      181 agcactcaga gcgcaggggg cactcgtggc agagcgactt ggccagctcg acctcgctcg
      241 gggactcgga gaagaacagg tctgggccga agactcgaca gggaacacgg tcggtcacgc
      301 gcatgcggtt gtcggccaac acggtgagcg tcatgaaggt cacctccagc taggccctcg
      361 gacggccgga tcacgaccga caccagggcg gaagatcagg gaaagacaaa gaccgcggat
      421 cccgggtcgg gttccgcggc ctgagggcca gccagtcgag gtgctagacc agcgggctcc
      481 aggctcgggg agcacgagaa gagatctcag tgatgaccgc aatggcggcg ccaaagccac
      541 cggcgaccag gagacgatca cggtgagcca ggttgacggc gatgcgatcg ccggcggtcg
      601 gggaggcgaa gccgcccatg ccataggtgg cagccgcgcc gacgccgttg accaatgtcg
      661 tcgtgctcac gggtgccacc tcctcactct ctcgatcgtg gccctgcctg ccgcggggcc
      721 ccgctgcggg ccgactcaca ggggccggcc acaagcgcgt aggcgacgat agaccccgcc
      781 gcggtcagga gcaaccatat tttttagtca ttacccgggc ggccagaacc gagccgctcc
      841 gggtcacccc gatgacgtct cgccgaccag gtcgaggacc tccgcgccgt agcggtcgag
      901 cttggtgcgc cccacgccgg gaacggtcgc cagcgcctcc agcgtcgtcg ggcgcagctc
      961 ggcgatcgct gtcagtgtcg cgtcggtgaa gacgacaaag gccggcaccg aggcgacccg
     1021 ggcccgctcc agccgccagg cccgcagccg ttcgaaggtc gcctcgtcga tgtcgaccgg
     1081 gcagtcgacg cagcgaccga gcttgcgggc caccgccgag gacagcggcc gcccgcagac
     1141 ccggcacacg gccggcgggc gccggcgccc cgtgcgggtg cgcccggccc cagcacgctc
     1201 cacatcccgc tggtcggcgg ggcgcaggcc atcgagaaag cgcgaggggc gccgggtcgg
     1261 tgccccaccg ggggcacggg agcgggtcca cgacagcgcc aggtgctcgc gggcccgggt
     1321 gacgccgacg tagagcagcc ggcactcctc ggcgaccgcc tcgggcgtct gggcatagga
     1381 gatcggaacc atgccctcgg tcagtccgac gaggaacacc gcgtcccact ccaggccctt
     1441 ggccgcgtgc agcgaggcca gcgtgacggc atcgacgacc ggcgcgtgcg cggtggcggc
     1501 gcggtcgtcg agctcgtcaa ccagctcggc cagcccggcg gccggctggc gggccagcag
     1561 gttctcggcc agccccacca gcgcggcgag cgactcccac cggtcgcggg ccgcgccggc
     1621 gccctgggga ggggtggtgc tccagcccat ccccccgagg accgcgcgca ccacgtcgac
     1681 gaggggctcc tcggtgcgcc cggaccgggc cgccgcccgc agcagcaggt gcgcctcacg
     1741 cacctcacgg cgctcgaaga accgctcgcc gccgcgcagc aggaacggca cacctgcctc
     1801 ggtcagcgcc tcctcgtagg ccgccgactg cccgttggtg cggaacagga cggcgatctc
     1861 atgagccggc acgccggcgg cgaccagcgc ccggatccgt tcggcgaccc catgagcctc
     1921 ggccggctcg tcggcgtact cgcaaaacgt cggcgccggc ccgctctcac gtacggcgcg
     1981 cagctccacc ccgtcgccgg cgggtagccc cgccttcagc acaccgttgg ccagcccgac
     2041 cacctgcggg gtggaccggt agtcccgcac gagcctgacc acggtggcgg cgggatggcg
     2101 cgtgcggaaa cccagcaggt aggacggggt ggccccggcg aacgagtaga tggtctgacc
     2161 cgggtcaccg acgacgcaga ggctgtcgcg ccccccgagc cactgctcga ggagctgctg
     2221 ctgcagcggt gagacgtcct ggtactcgtc gacgacgaag tggcggtact gcgcccgcac
     2281 tgcctcggcg acctcggcgt gctcggcgag caggccgacg gtgatgagca ggacgtcctc
     2341 aaagtccatg acgtcgcgct cacgcttgat ctcgtcgtag ccgccgagca gccgctcgac
     2401 ggtcgtggcg tccagcccgg ccggcggggt gcgcccggca cgcgcgagcg cggcgacgta
     2461 gccgtcgcgg ggcaccaggc tcaccgcggc ccactccacc tcggcggcga ggtcacgcag
     2521 gtccgtgcga tgcgggcgca gtcccagccg acccgccgcc tcggcgatca gggacacctt
     2581 ggccgcgatc acccgaggcg gggtgccgcc gatggtccgg ggccagaagt actgcagctg
     2641 gcgcagggct gccgagtgga acgtgcgagc ctgcaccccc ggcaccccga gtgcgcgcag
     2701 ccgcccgcgc agctcgcccg ccgcgcggtt ggtgaacgtc accgccagca cgtgctgggg
     2761 ggcatagacg ccggccagca cgccgtacgc gatccggtgg gtgatcgccc gggtcttgcc
     2821 ggtgcccgcc ccggcgagca cgcacaccgg cccggtcagc gcggtggcca ccgcccgctg
     2881 ctcggggtcg agcccagcca gcaccgcgtc ggcacctgcc ggctcggcca agtcaggcga
     2941 gatcgtgctc accgggccat cctcccagcc cgcgccgacc gctccctgcg ggatgatctg
     3001 ggcgtatcac gtcgttgcac agacaagaga tacgccttcc cgcacacgaa cgagaggacc
     3061 ccggcatgac ggccaacgcc gagatcacga tgtacagcac cacgtggtgt ggctactgcc
     3121 ggcggctgaa gtcgcagatg gaccgcgagg gcatcgccta ccgcgaggtc aacatcgagc
     3181 tcgacccgcg ggccgccgac ttcgtgatga gcgtcaacgg gggcaaccag acggtgccga
     3241 ccgtgctgtt ccccgacggc agcgcgttga ccaatccgtc actggccgag gtcaagggca
     3301 agctggcggc ctgagccggc tcaccaggca aggtcctcgc ccaccgggcc gccgtaccag
     3361 ttctccacca gccgccgggc gatcgagacc ggcgagggaa gcagcacgtc ccgggcggcc
     3421 accgcggcgg ccagctcggg ccgggtgaac cagcgggcgt cggagatctc gtggccgtcg
     3481 gggtgcgcct cgcagtcgat ggcgtgggcg tggaagccga gcatcaacga ggacgggaac
     3541 ggccacggct ggctgcccat gtaggtcacc acgccgacgt gcaggccgac ctcctcgagc
     3601 acctcgcggc gcaccgccgc ctcgagcgac tcccctggct cgacgaaccc ggccaacgtc
     3661 gagaaccgac cgttgggcca gcgggcctgg tgaccgagca gcagccggtc gtcgtcgtcg
     3721 accaccgtca tgatcaccgc gggatcggtg cgcggatagt gctcgctgcc gtcggcgggg
     3781 caccggcgta ccgatccgga cagcaccggc tcggtgggag caccacaccg cgggcagtgg
     3841 ctgtgcgtct cgtgccagtt ggccatcgcc gccgcgtgga cgaacagccc ggcctcggtg
     3901 tcggtcagca gggcgcccac ctcgcgcagc ccgacccacc gagtgccgtt ggcctcgccg
     3961 aggtccgggt tgcgcccggg gtcggtcacg acggcgaagc aggcgacgtc gtcggcgtcc
     4021 acaccgagca ggatctgcag gccgtcgggg gcctggtcgg gagccacgag caggagggtc
     4081 ggcgcggcgc cgtcgtggac gaccctcgcc tgcccatgtt cgagcaccag cacccgggtc
     4141 gccgggtcgg cccagcgggc gtccagccac gcctggtcgg tgcgatggtg agcggcccgg
     4201 tccacgctgg cccgcgagag cgcaagctgc gtcagaccgg ccgatgagga catggaccga
     4261 ctctaggcca acccgcccga atggccgccg cgcaggtgat ggcaggcgac gcgccgaagc
     4321 catgccatca tcggcgccca tgagcactca catcggcgcg gcggcagacg agatctccga
     4381 gcacgtcctg ctgcccggtg acccgttgcg ggccaagtgg atcgccgaga acttcctcac
     4441 cgatgctcac tgctactcga gcgtgcgcaa catgctgggc ttcaccggca cctaccgcgg
     4501 cgagcgcatc tctgtgcagg gcaccgggat gggccagccg tcgctgtcga tctacgtgca
     4561 cgagctgtgc acggagtacg gcgcgcgcac cctggtccgg gtcggctcct gcggtgcgct
     4621 ggcgcccgac ctgagcctgc gtgatgtggt gctcgcgatg agcgcgagca ccgactcggc
     4681 gatcaaccac gtgcgcttcg agggcatcga cttcgccccg acggccgact tcgagctgct
     4741 gcgtgcggcc cacgacgcgg cccgggcccg cgggctcgac gtgcacgtcg ggccggtggc
     4801 ctcctgggac gtgttctact ccgaccgtcc cgagctgctg cgccggctcg cggagtacgg
     4861 cgtgctcgcg gtcgagatgg agaccgccgc gctctacacg ctcgcggcga agtaccacgc
     4921 acgcggcctg gcgctgctca cggtcagcga ctcgctggtc tccggggagg agacgtcggc
     4981 gcaagaacgc gagcagacct ttggcgagat ggtcgagctc gcgctggact cggtgctctc
     5041 gcttgccaag accgacggct gagctgctca gggctcctcg ccgtgctgaa gtcggcgtag
     5101 cgcaacagcg agcgctcgac gccgctccgc gagctcgtcg acggtcagat cgcgtcctgc
     5161 tcgaaaggtc gccaaccgca gctccttcgc ccgatcggcc agcagaagct ggcgcccgtc
     5221 ggcggcgcgc gccagatctt cgctggcatc ggcggcccgg gtgatgctgg ccagcagctc
     5281 gccagcctgg ctgattgcct ggctgattgc ctggtgcgcc tcgtcgttgt cagcgccgtc
     5341 aatgccggcg gacgcgagct cacggcgcag gtcatcggcc tccaacagga gcgtcactgc
     5401 ctgccacagc gctgtcgtca ctgcgctgtc aagctctgcc ccgtcgccaa gcccgtccgg
     5461 catcctggcc gccgcgatca gtgcccgacg tcgagcgtgt gcggccgcgg tgagctgaac
     5521 gcgccatgag ggagacaggg tcgtgaccat gacgagtcgt cggttggtga tccgccgcgc
     5581 gacgcggctg gcccaccacg cggaggcaac gagaccggcc gccaccagca tcgccggccc
     5641 tggcccgaac gcggagctcg cgatggtcag cccgccgcca atggtcacca gcaccgcctg
     5701 accgcgagga cgggacacgg ccgtgtccca ccatcggaac gcccgcggga tcggctccat
     5761 cgcgatggcc gcgaactggc ctgcctcgtc tacgaacacc acatggtctg gcccggtgcg
     5821 ccttcgccac acgcttcccc acgcgctcga tgatcgatcg ggccggagcc tagccgcgga
     5881 tgcttcccac ctcggggagc gcgtcgacga gggcgagcag gtcagcctcg tcgaacaggt
     5941 cgaccggacg gacggtctgt ccggtggcgg cgtaaaagaa cgccgcgctg acccgctcga
     6001 gcggcacagc gcgcaggcgc gaccaggcga gccgatagac agccagctgt acggcgcgca
     6061 cccgcgccgc gtcgccggtc ggtggtcgac cggtcttcca gtcgaccacc tcaaagccgc
     6121 tgtcggcggt tgcgaacacc gcgtcggccc gaccgcggag cagcaccggg ccgatcgtcg
     6181 tctcgaaggc gacctcgacc tcgagcggca gccgatcagc ccactcgctg gccaggaacg
     6241 tcgccttgag cgccgcgagg tcctcatcgc cggtggcgct ctcgtcggca gccccgggca
     6301 ggtcgaacag gtcgaccatc gcagcctgcc cgaaccgctg ctcgagccag gtgtggaacg
     6361 ccgtgccccg gcgagcggcc gggcgtggtg gcccgggcac cgggcgacgg atgtcgcggg
     6421 ccagggcgtc ggggtcctcg gcgaggcgga ccagccgtga ggtcgacagg tgggccggca
     6481 gcgccacctc gatgacctcg cgacgcgcgc gggcacgctc ggccagcagc aggtcgacct
     6541 cggccgacca ggggtcgtcg ggctcggtcg tctcggtcag gcaggcaacg gcgggcgggt
     6601 cggtcgggtc ggcggggtcg gtcgggtcgg cggggtccgt ctggtcgccc tcgtcgtgcg
     6661 cggccgccgt cagcgccatc tgcaccaggg cggcgccggc ctccaccacg gcgcggcgct
     6721 cgccgagcgg gtcgaggggc caggggtggg tcgcggcgct ggcggtgcgc ggattagtcc
     6781 cgtcggggcg aggcacccag gtgccccgct cgaccacgcc ggcgagctgt gcggtcgtgg
     6841 gctcgaccat ctcggcgagg aagcgcgaga cctcgcgggg agcgcccgcg tcgtcccacc
     6901 ggtgcccggt gagcagcagc agagagcggg ctctggtgac ggcgacatag gccagccgcc
     6961 gctcctcggc cagctcgtgc gaaccggcgc tgccccggaa cgactgccac tgctccttga
     7021 gctcctcctg gctggcgcat cggtcccagc gccacacagg caggcccgcg gcgtcacctc
     7081 gaagcgggaa gggcagcggg ccgagcccgc tcagccaccc gccgtcgacg tcgccgcagg
     7141 ccgggaagat cccttcgacc agccctggta cggcgacgac gtcccactcc agtcccttgg
     7201 cggcgtgcac ggtcagcacc tgcaccgcct ggtcggtgac gtcgacctcc ccgggttgca
     7261 ggccccgctc acgcagctcg gccgcctgca gccaggccag gaaaccaccc aggctggccg
     7321 tctgcgccga gtcggcgaac cctgcggcca cgtcaaggaa cgcgtcgaga tgggcccggg
     7381 ccgcggcggc cgaccgaccg ggccgggcgg cgacctcgat gtcgatgccg atcgtgcgct
     7441 cgacgtcgac gacgatctcg ggcagcgatg ccccgacccg ggcccgtagc ccgcgcagct
     7501 cggcggccag cgaggccagc cggcgcagcg ccggctcgga caggcccaca ccgtcggcgt
     7561 cgcgccaggg ccgcctgggc agctcgtcga gggcctccac gatgctgcgc tcgtcgacgg
     7621 cgtcgggcac cggcgtgtcg cccgcctccg ccggcccctc gaccggctca cgcccgccag
     7681 cgccggccgc cgccctggcc cgggccagcc gccgtgacca ccggcccagc ccctcgaggt
     7741 cgcgcgggcc cagccgccag cgtggtccgg tcagcagccg catcagtgcg tcgccccggg
     7801 tcgggtcgtg gaccacctgc agggtggcca ccagatcggc cacctcgggg gtcgacagca
     7861 gcccgcccag cccgaccacc tcgacgggca ggccgcgggc ccgcagcgcc gcctcgaccg
     7921 cggggaactg gctgcgcttg cggcacagca ccgccacagt gcggcgggcc cggccggccg
     7981 ccgcaccggg gtcgtcggcc cgccacacgc gcgccagggc atcggcgatc gcggcggcct
     8041 cgtcctcaac cgtctcgtgg atggcggcca ccacccgccc ggacccg
//
LOCUS       DATIBB010000118         8536 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_1117975, whole genome shotgun sequence.
ACCESSION   DATIBB010000118 DATIBB010000000
VERSION     DATIBB010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 8536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8536
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1117975"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..937
                     /gene="nusA"
                     /locus_tag="VLC50_04545"
     CDS             <1..937
                     /gene="nusA"
                     /locus_tag="VLC50_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013154601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HSF26768.1"
                     /translation="VTVWATETDEEDNPVGEYDDTPTGFGRIAATTARQVILQRLRDA
                     EDEQVFGEFAGKEGDIVSGIVQQGSNPRDVMVNLGRFEGLLPPAEQVPGESYEHGSRL
                     RCYVVSVRRGPRGPQVTLSRTHPNLVKKLFALEVPEIADGTVEIAAIAREAGHRTKMA
                     VHSTRAGVNAKGACIGPMGQRVRAVMTELHGEKIDIVDWSEDPAQMVAHALSPARVSQ
                     VIVVDAAARSARVIVPDYQLSLAIGKEGQNARLAARLTGWRIDIRSDAATTPVTAPAT
                     VPATTSTTAPSAAPVSAPTSPATPAGPGGTDRSAG"
     gene            970..1284
                     /locus_tag="VLC50_04550"
     CDS             970..1284
                     /locus_tag="VLC50_04550"
                     /inference="COORDINATES: protein motif:HMM:NF016204.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HSF26769.1"
                     /translation="MPAHVPIRTCVGCRDKSAAPDLLRVALTWADDRPVIVPDRRARI
                     PGRGAWLHPVLRCLDQAERRRVFGRALRIPAGAAAPDTTGLRAWITEQEHGSSARVSP
                     TG"
     gene            1431..4250
                     /gene="infB"
                     /locus_tag="VLC50_04555"
     CDS             1431..4250
                     /gene="infB"
                     /locus_tag="VLC50_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013770692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HSF26770.1"
                     /translation="MVAKVRVYELAKEIGVESKVVMAKLQELGEFVRSASSTVEAPVV
                     KKLREAFPAAGGAAPAPARPARKAPTAPADGAVPAPAAPGRPAPAGAAPKPGPAAPPA
                     PAAPAAPASPASPARPAAGPAPAAPAAPVAPAAGPAPAAPTPAPRPAGPRPAGPRPGN
                     NPFSSGASTGMQRPSAPRPAGPRPGNNPYTSGGSTGMPRPSAPRPGGAPRPGAPAGGG
                     MPPRPGGPRPNPGMMPPRPANRPGPGGAGRPGAGTRGGGRPGTGTGGRPGGGGGRPGG
                     GTGGGGFAGRPGGGGGPRGRGGTAGAFGRPGGAPRRGRKSKKQRRQEFDNMAAPSIGG
                     VQIPRGDGSTAVRLPRGASLADFAERIGANPASLVTVLFHLGEMATATQSLDEDTFKL
                     LGAELGYDIQVVSPEDEDRELLGTFDIDLAAEADADDEDALRPRPPVVTVMGHVDHGK
                     TKLLDAIRHTNVAAKEAGGITQHIGAYQVRTPHEGQDRAITFIDTPGHEAFTAMRARG
                     AQSTDIAILVVAADDGVMPQTIEALNHAQAAGVPIVVAVNKVDKEGANPAKIRQQLTE
                     YNLVAEEYGGDTMFVDVSALQRKGIDELLEAVLLTADAALDLRANPDKAARGVAIEAH
                     LDRGRGPVATVLVQSGTLHVGDAIVAGTAFGRVRAMLDELGSQLDEATPARPVQVLGF
                     TSVPGAGDKFLVTSDDRTARQIAEKREAAERNAALAKARKRVSLEDWLQKSKVDTLNL
                     ILKGDGSGSVEALEDALLNLEVGEEVALRVIDRGVGAITQNDVNLAVASDAVIIGFNV
                     RPAERVTELIDREGVDVRYYSVIYQAIEEVEAALKGMLKPEYEEAQLGTAEIREVFRS
                     SKFGNIAGALVRSGTIVRNSKARLVRDGVVVAENLSIDSLRRFKDDATEVREGYECGI
                     GLGSFNDIKVDDVIETYEMREKPRT"
     gene            4320..4619
                     /locus_tag="VLC50_04560"
     CDS             4320..4619
                     /locus_tag="VLC50_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018562639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HSF26771.1"
                     /translation="MYVGSLSLDLLLGDVRSLKAKRSVVRPIVAELARRFPTAAVAEV
                     DHLDLHRRAGLGVAVVAPDPAHARSLLEACERLVSARPEVELLSARHQLWNNDAP"
     gene            4669..5142
                     /gene="rbfA"
                     /locus_tag="VLC50_04565"
     CDS             4669..5142
                     /gene="rbfA"
                     /locus_tag="VLC50_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014055275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HSF26772.1"
                     /translation="MVDAARARKLADRIRVIVAQMLETRVKDPRLGFVTITDTRITND
                     LQQATVFYTVYGDEHERASTAAALESAKGVLRSEVGRQTGVRHTPSLTFVADVVPDTA
                     RHIEDLLVRAQAADAEVMRASAGAAYAGEADPYRAPREEEELADAGEPDRDGAPE"
     gene            5139..6017
                     /gene="truB"
                     /locus_tag="VLC50_04570"
     CDS             5139..6017
                     /gene="truB"
                     /locus_tag="VLC50_04570"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005455533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HSF26773.1"
                     /translation="MSSPDGLLVIDKPAGLTSHDVVARVRRLAGTRKVGHAGTLDPMA
                     TGVLVVGVGRATRLLGHLSLTEKAYDATIRLGVTTTTDDADGEVVSRAEAGAVTEAAV
                     RAGMARLTGEIEQVPSSVSAVKVDGRRAYARVRAGEAVELAPRRVTISQFDLLAVRRA
                     GPVLDLDVRVGCSSGTYVRALARDLGADLGVGGHLTALRRTRVGPYHLALARTLDELA
                     EHFDVVPMGEAAAAAFPVRRLDADELDAVRHGRTIAAAGLGAGPVAALAADGSLVALL
                     EESGPVARPLAVLAPA"
     gene            complement(6024..6974)
                     /locus_tag="VLC50_04575"
     CDS             complement(6024..6974)
                     /locus_tag="VLC50_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020393463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF26774.1"
                     /translation="MSHLKRFVVIVAALVLTISAAAPFSDSVPLPDDFAPEGIAVGTG
                     STFYVGSLVDGDIYRGDLRSGEGAVFIDAPAGRQAAGIKVDEAHHRLFVAGVTTGHAY
                     VYDTRDGSPLADLTLATGPAFINDVVVTKGGAYFTDSLNPVLYKVPIAPNGALGPPET
                     IELTGPASTILDFPNLNGITATPSGDTLIVGHSSLGAVLTVDPDTGASRQIAVTGGSL
                     TPGTNDGILLDGKTLWVVENFANRLLEIRLAPDLSSGRITATVTNADVDGALRVPTTV
                     AEHGHLLAIVNARFDLGLPPPLGTGAPPGTDYDVVLVPKP"
     gene            7105..8349
                     /gene="kynU"
                     /locus_tag="VLC50_04580"
     CDS             7105..8349
                     /gene="kynU"
                     /locus_tag="VLC50_04580"
                     /EC_number="3.7.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030429 - kynureninase activity [Evidence
                     IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kynureninase"
                     /protein_id="HSF26775.1"
                     /translation="MPIALAAARRRAERLDDADPLRHVRARFSLPEGVIYLDGNSLGA
                     LPAHVPAVVEDVVRRQWGQVLIASWNTEDWWDAPARVGARIARLVGAGAEEVVVADST
                     SVNLFKVLVAAARMRPERRTVLVESGVFPTDLYVADGVADLLDLTVRRVSPHELADTL
                     DEDVAVVVLSQVDYRTGEAYDVAEITAAVHEAGALMVWDISHSVGVLPVGFDEHGVDF
                     AVGATYKYLNGGPGAPAFVVVAARHQDRLASPLTGWNGHARPFAMEGTFEPAPGMTRL
                     RVGTPPVVSLLVLEAALTVFDDLDLAAVRDRSLSLTGLFLDLADAHLVPAGFTVATPL
                     EPQRRGSQVSLRHPQAYGVVRALAARGVVGDFREPDVVRLGFAPLYVRHVDVVAAVES
                     LLAVAATGEADDPRWSARDLVT"
     gene            8413..>8536
                     /locus_tag="VLC50_04585"
     CDS             8413..>8536
                     /locus_tag="VLC50_04585"
                     /inference="COORDINATES: protein motif:HMM:NF018299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HSF26776.1"
                     /translation="MQRWQRVSDVPSSWPGSAVTIGVFDGVHRGHRVVVSAMVEQ"
BASE COUNT         1131 a         3127 c         3158 g         1120 t
ORIGIN      
        1 cgtcacggtc tgggccaccg agaccgacga ggaggacaac ccggtcggcg agtacgacga
       61 cacacccacc gggttcggcc ggatcgcggc cacgaccgcc cggcaggtga tcctccagcg
      121 gctgcgtgac gccgaggacg agcaggtgtt cggggagttc gccggcaagg agggcgacat
      181 cgtctcgggc atcgtccagc agggcagcaa cccccgcgac gtgatggtca acctcggccg
      241 gttcgagggc ctgctgccgc cggccgagca ggtgccgggg gagagctacg agcacggctc
      301 ccggctgcgc tgctacgtcg tgtcggtgcg ccgcggcccg cgcgggccgc aggtcaccct
      361 ctcgcgcacc caccccaacc tggtcaagaa gctgttcgcc ctcgaggtgc ccgagatcgc
      421 cgacggcacc gtggagatcg cggcgatcgc ccgcgaggcc ggccaccgca ccaagatggc
      481 cgtgcactcg acccgcgccg gggtcaacgc caagggtgcc tgcatcggtc cgatgggcca
      541 gcgggtgcgg gcggtcatga ccgagctgca cggcgagaag atcgacatcg tcgactggtc
      601 ggaggacccg gcgcagatgg tggcgcacgc cctgtcgccg gcccgggtgt cccaggtcat
      661 cgtggtggac gcggccgccc gctcggcccg ggtgatcgtg cccgactacc agctctcgct
      721 cgcgatcggc aaggagggcc agaacgcccg gctggcggcc cgtctcaccg gctggcgcat
      781 cgacatccgt tccgacgccg cgacgactcc ggtcaccgcc ccggccaccg tccccgcgac
      841 gacctcgacg accgctccga gcgccgcccc cgtgtccgct ccgacctcgc ccgcgacgcc
      901 ggccggtccg ggcgggacag accgctcggc ggggtagact tcgcggtggg cgtccgacct
      961 gatcctggca tgcccgcgca cgtcccgatc cggacctgcg tgggttgtcg tgacaagtcg
     1021 gccgcgcccg acttgctccg ggtcgcgctg acctgggccg acgaccgccc ggtcatcgtc
     1081 cctgaccggc gggccaggat ccctggacga ggtgcgtggc tgcaccccgt cctccggtgc
     1141 ctcgaccagg ccgagcgtcg ccgggtgttt ggccgagccc tccggatccc ggcgggtgcg
     1201 gcagcgcccg acacgaccgg cctccgggcg tggatcacgg agcaggagca cgggtcatcc
     1261 gcgcgggtct ccccgaccgg atgacctctg ccgtgaccgg accaggtcgc ggcagaccgt
     1321 cctagtcgga agcgggtcga agcgatgagc ccccgatgag cacgcagcta tgactgcccc
     1381 cgtgcactaa gacggtccgc gcgccagcag gcccggaccg agacaggaga attgtggcca
     1441 aggtccgggt ctacgagctc gccaaggaga ttggcgtcga gagcaaggtc gtcatggcca
     1501 agctccagga gctcggtgag ttcgtccggt cggcgtcctc cacggtggag gcaccggtcg
     1561 tcaagaagct tcgcgaggcg ttccccgctg ctggcggggc tgcccccgcg ccggcgcgtc
     1621 ctgcgcgcaa ggcccccacc gcaccggcag acggtgccgt ccccgcgccc gccgctcccg
     1681 gtcgccccgc accggctggc gctgcgccca agcccggtcc ggcggctccg cccgcgccag
     1741 ctgctcccgc ggctccggcc agcccggcca gcccggcccg cccggctgct ggcccggcgc
     1801 cggctgcccc agccgcaccc gtggcaccgg ccgcaggtcc ggcgccggcc gcgccgacgc
     1861 ccgcgccgcg tccggcgggg ccccgcccgg cgggcccgcg cccgggcaac aacccgttca
     1921 gctcgggtgc cagcaccggc atgcagcggc ccagcgcgcc gcgtccggcc ggcccgcgcc
     1981 cgggcaacaa cccctacacg tcaggcggca gcacgggcat gccgcgtccc tcggccccgc
     2041 ggcccggtgg ggcgcctcga cctggggcac cggccggtgg tggcatgccg ccgcgcccgg
     2101 gcggtccccg gcccaacccc ggcatgatgc cgcctcgccc ggccaaccgg ccgggtcccg
     2161 gcggtgccgg tcggcccggt gccggcaccc gcggtggcgg ccgtcccggc accggcaccg
     2221 gtgggcgccc cggtggcggc ggaggtcgtc ctggcggcgg caccggtggc ggcggcttcg
     2281 ccggccggcc cggcggaggc ggcggcccgc gtggccgtgg cggcacagcc ggggcgttcg
     2341 gtcgcccggg cggcgctccg cgccgcgggc gcaagtccaa gaagcagcgt cgccaagagt
     2401 tcgacaacat ggcggctccg tcgatcggcg gcgtgcagat cccgcgcggt gacggcagca
     2461 cggcggttcg gctgccgcgc ggcgcctcgc tggccgactt cgccgagcgc atcggcgcca
     2521 atccggcgtc gctggtgacc gtgctgttcc acctgggcga gatggccacg gccacccagt
     2581 cgctcgatga ggacacgttc aagctgctcg gcgccgagct gggttatgac atccaggtcg
     2641 tctcgcccga ggacgaggac cgcgagctgc tcggcacctt cgacatcgac ctggccgccg
     2701 aggccgacgc cgacgacgag gacgcgctgc gcccccggcc gccggtcgtc acggtcatgg
     2761 gtcacgtcga ccacggcaag accaagctgc tcgacgcgat ccggcacacc aacgtggcgg
     2821 ccaaggaagc cggtggcatc acccagcaca tcggcgccta ccaggtgcgc acgccgcacg
     2881 agggccagga ccgcgcgatc acgttcatcg acacccccgg ccacgaggcg ttcaccgcga
     2941 tgcgtgcccg tggtgcccag tcgaccgaca tcgcgatcct cgtggtcgcg gccgacgacg
     3001 gcgtgatgcc gcagacgatc gaggcgctca accacgccca ggccgccggc gtgccgatcg
     3061 tggtcgcggt caacaaggtg gacaaggagg gcgccaaccc cgccaagatc cgccagcagc
     3121 tcaccgagta caacctggtc gccgaggagt acggcggcga cacgatgttc gtcgacgtgt
     3181 cggcgctgca gcgcaagggc atcgacgagc tgctcgaggc ggtgctgctc acggctgacg
     3241 cggcgctcga cctgcgggcc aaccccgaca aggcggcgcg cggcgtggcc atcgaggccc
     3301 acctcgaccg cggtcgcggt ccggtggcca cggtgctcgt ccagagcggc accctgcacg
     3361 tcggcgacgc gatcgtggcc ggcaccgcgt ttggccgggt ccgggccatg ctcgacgagc
     3421 tgggcagcca gctcgacgag gccaccccgg cccgtccggt gcaggtgctc gggttcacgt
     3481 cggtgcccgg tgccggtgac aagttcctcg tcaccagcga cgaccgcacc gcccgccaga
     3541 tcgccgagaa gcgcgaggcg gccgagcgaa acgccgcgct ggccaaggcc cgcaagcggg
     3601 tctcgctcga ggactggctg cagaagagca aggtcgacac cctcaacctc atcctcaagg
     3661 gcgacgggtc cggctcggtc gaggcgctcg aggacgccct gctcaacctc gaggtgggcg
     3721 aggaggtcgc gctgcgggtg atcgaccgcg gtgtcggtgc catcacccag aacgacgtca
     3781 acctggccgt cgcctccgac gccgtgatca tcggcttcaa cgtgcggccg gccgagcggg
     3841 tcaccgagct gatcgaccgc gagggcgtcg acgtgcgcta ctactcggtg atctaccagg
     3901 cgatcgaaga ggtcgaggcc gcgctcaagg gcatgctcaa gcccgagtac gaagaggcgc
     3961 agctgggcac cgccgagatc cgggaggtgt tccggtcgag caagttcggc aacatcgccg
     4021 gtgccctcgt ccggtccggc acgatcgtgc gcaactccaa ggcccggctc gtgcgcgacg
     4081 gcgtcgtcgt ggccgagaac ctcagcatcg actcgttgcg ccggttcaag gacgacgcga
     4141 ccgaggtgcg cgagggctac gagtgcggca tcggcctcgg gtcgttcaac gacatcaagg
     4201 tcgacgacgt gatcgagacc tacgagatga gggagaagcc gcgcacctga cccggtgacc
     4261 gaccgtcgcg cccacccggc tccggccggg cgggtcgcgg cccgcggtgg ggagtctcga
     4321 tgtacgtcgg gagcctgagc ctcgacctgc tcctaggcga cgtgcgctcg ctcaaggcca
     4381 agcgttcggt cgtccggccg atcgtggccg agctggcccg gcgcttcccc accgccgcgg
     4441 tggccgaggt cgaccacctc gacctgcacc ggcgggcggg gctgggggtg gcggtggtcg
     4501 cacccgaccc ggcccacgcc cgcagcctgc tcgaggcctg cgagcgcctg gtctccgcgc
     4561 gccccgaggt cgagctgctc tcggcccgcc accagctgtg gaacaacgac gccccctagg
     4621 gcgacaagcc gccccccggg gcaccgatga gtgcggagga caggagccat ggtcgacgcg
     4681 gcgcgtgccc gcaagctggc tgaccggatc agggtcatag tcgctcagat gctggagacg
     4741 cgggtcaagg acccccggct cgggtttgtg acgatcaccg acacccggat caccaacgac
     4801 ctgcagcagg cgacggtctt ctacaccgtc tacggcgacg agcacgagcg ggccagcacc
     4861 gccgcggcgc tggagagcgc caagggcgtg ctgcgctcgg aggtgggccg gcagaccggg
     4921 gtgcggcaca ccccctcgct cacgttcgtc gccgatgtcg tgcccgacac ggcgcgccac
     4981 atcgaggacc tgctggtgcg ggcccaggct gccgacgccg aggtcatgcg tgcctccgcc
     5041 ggggccgcct acgcgggtga ggccgaccct taccgcgctc cccgggagga ggaggagctg
     5101 gccgacgccg gcgagccgga ccgggacggc gcgccggagt gagcagcccc gacgggctgc
     5161 tggtcatcga caagccggcg gggttgacct cccacgacgt cgtggctcgc gtgcgtcggc
     5221 tcgccggcac tcgcaaggtc ggtcacgccg gcacgctcga cccgatggcc accggggtgc
     5281 tggtggtcgg cgtcgggcgg gccacccggc tgctcgggca cctgagcctg accgagaagg
     5341 cctacgacgc gacgatccgc ctcggcgtca ccactaccac cgatgacgcc gacggcgagg
     5401 tcgtctctcg ggccgaggcc ggcgcggtca ccgaggcggc ggtgcgggcc gggatggccc
     5461 ggctgaccgg cgagatcgag caggtgccct cgtcggtgag cgcggtgaag gtcgacggac
     5521 ggcgggccta cgcccgggtg cgcgccggcg aggcggtcga gctggcgccg cgccgggtca
     5581 cgatcagcca gttcgacctg ctcgcggtcc gccgcgccgg cccggtgctc gacctcgacg
     5641 tgcgggtggg ctgctcgtca ggcacctacg tgcgcgcgct tgctcgcgac ctaggcgccg
     5701 acctgggcgt cggggggcac ctcacggcac tgcggcgcac ccgggtgggc ccctaccacc
     5761 tggccctggc ccgcacgctc gacgagctgg ccgagcactt cgacgtcgtg ccgatgggcg
     5821 aggcggccgc cgcggccttc ccggtgcgca ggctcgacgc cgacgagctc gacgccgtac
     5881 gccacggccg cacgatcgcc gcagccggcc tcggcgccgg tccggtggcc gcgctggccg
     5941 ccgacggctc gctggtcgcg ctgctcgagg agtcgggccc ggtggcccgc ccgctcgccg
     6001 tcctggcgcc cgcctgaggt ggctcagggc ttggggacga gcaccacgtc gtagtccgtg
     6061 ccgggcgggg caccggtgcc aagtggcggg ggcagcccga ggtcgaaccg ggcgttgacg
     6121 atcgcgagca gatggccgtg ctcggccacc gtggtgggca cccggagggc gccgtcgacg
     6181 tcagcgttgg tcaccgtggc ggtgatgcgc ccgctggaca ggtcgggagc cagcctgatc
     6241 tcaaggagcc ggttggcgaa gttctccacc acccacaagg tcttgccgtc gagcaggatc
     6301 ccgtcgttgg tgcccggagt gagcgagccc ccggtgacgg cgatctggcg actggcaccg
     6361 gtgtcggggt ccacggtgag cacggctccc aacgagctgt gcccgactat cagcgtgtcg
     6421 ccggacggtg tggcggtgat cccgttgagg ttggggaagt cgaggatggt gctcgccggg
     6481 ccggtgagct cgatcgtctc cggcggcccg agcgcaccgt tgggcgcgat cggcaccttg
     6541 tagagcaccg ggttgagcga gtcggtgaag taggccccgc ccttggtgac gaccacgtcg
     6601 ttgatgaagg ccggcccggt cgccagcgtc aggtcggcca gcggtgaccc gtcgcgggtg
     6661 tcgtagacgt aggcatggcc ggtggtcacc ccggcgacga acaaccggtg gtgggcctcg
     6721 tcgaccttga tcccggcggc ctgccgtccg gccggagcgt cgatgaacac ggccccctcg
     6781 ccgctgcgca ggtcaccccg gtagatgtcg ccgtcgacga gcgacccgac gtagaaggtg
     6841 gacccggtgc cgaccgcgat gccctccggg gcgaagtcgt cgggcaacgg caccgagtcg
     6901 gagaacggcg ccgcggcgct gatggtcagc accagcgcgg cgacgatgac cacgaatcgc
     6961 ttgaggtgtg acatctcggg cctccactcc gccttgtcga ggagagccgc acggctggct
     7021 gctgcctccg gcctagacct cgccggtctc cgcgtccagg gcttgcggca atcgcgggcg
     7081 tcgtcccgtc gctagggtct ggccgtgccc atcgcgctgg ccgcggcccg gcgacgggcc
     7141 gagcggctcg acgacgccga cccgctgcga cacgtgcggg cccggttcag cctgcccgag
     7201 ggcgtgatct acctcgacgg caactcgttg ggcgcgctgc ccgcgcacgt gcccgcggtg
     7261 gtcgaggacg tcgtacgacg ccagtggggc caggtgctga tcgcctcgtg gaacaccgag
     7321 gactggtggg atgccccggc ccgcgtgggg gctcggatcg cccggctggt cggcgcgggc
     7381 gccgaggagg tggtggtcgc cgactccacg tcggtcaacc tgttcaaggt gctggtggcg
     7441 gcggcccgga tgcgcccgga gcggcgcacg gtgctcgtgg agtcgggcgt gttcccgacc
     7501 gacctctacg tcgccgacgg ggtcgccgac ctgctcgacc tcacggtccg gcgggtgtcg
     7561 ccccacgagc tggccgacac gctcgacgag gacgtcgcgg tggtggtgct cagccaggtc
     7621 gactaccgca ccggcgaggc ctacgacgtg gccgagatca ccgccgcggt ccacgaggcg
     7681 ggcgcgctga tggtctggga catctcgcac tcggtcgggg tgctgccggt cggcttcgac
     7741 gagcacggcg tggacttcgc ggtcggcgcc acctacaagt acctcaacgg cgggcccggc
     7801 gcaccggcgt tcgtcgtggt cgccgctcgg caccaggacc ggctggccag cccgctgacg
     7861 ggctggaacg gccacgcccg cccgttcgcg atggagggca ccttcgagcc ggcgccgggg
     7921 atgacccggc tgcgggtggg caccccgccg gtggtctcgc tgctcgtgct ggaggcggcg
     7981 ctgaccgtct tcgacgacct cgacctggcc gccgtacgcg atcgcagcct ctcgctgacc
     8041 gggcttttcc tcgacctggc cgatgcccac ctggtgccgg ccgggttcac cgtcgcgacc
     8101 ccgctcgagc cgcagcggcg cggcagccag gtctcgctgc gccatcccca ggcctacggc
     8161 gtggtccggg ctctggcggc ccgcggggtg gtcggcgact tccgtgagcc cgacgtggtg
     8221 cggctgggct tcgccccgct ctacgtgcgc cacgtcgacg tggtggctgc ggtcgagtcg
     8281 ctgctcgcgg tggccgcgac gggcgaggcg gacgacccgc ggtggtccgc gcgcgacctg
     8341 gtgacctgag cggctccgcc gcccggatgg ccgcggtgat cgttctcgtc gcgccgtggc
     8401 aggcttgccg tcgtgcagcg ctggcagcga gtctccgacg tcccctcgag ctggcccggc
     8461 tccgcggtca ccatcggcgt gttcgatggc gtgcaccgcg gccaccgggt cgtggtcagc
     8521 gcgatggtcg agcagg
//
LOCUS       DATIBB010000119         4442 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_461050, whole genome shotgun sequence.
ACCESSION   DATIBB010000119 DATIBB010000000
VERSION     DATIBB010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 4442)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4442)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4442
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_461050"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..178)
                     /locus_tag="VLC50_04590"
     CDS             complement(<1..178)
                     /locus_tag="VLC50_04590"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017542902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HSF26777.1"
                     /translation="MSDRIGVHGLRGRGRHGVFDFEREAGQEFVVDVELCVDTRAAAD
                     SDDLAQTIDYGRLAD"
     gene            complement(175..642)
                     /locus_tag="VLC50_04595"
     CDS             complement(175..642)
                     /locus_tag="VLC50_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018551209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HSF26778.1"
                     /translation="MSSAGDADDAAAITAANAAFYQAIEGADLDLMGSIWDTADDVRC
                     VHPGTAMVNGRSAVMRSWAVVMAGTPYIQFFITDVGVSLVGDVALVVCNENVLTGVET
                     PDGSPSGFAGGKAVATNVYRRTPAGWRLWAHHSSPVMSASDTPDGDEQEDQRG"
     gene            complement(639..1472)
                     /gene="folP"
                     /locus_tag="VLC50_04600"
     CDS             complement(639..1472)
                     /gene="folP"
                     /locus_tag="VLC50_04600"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018385819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HSF26779.1"
                     /translation="MGVVNVTPDSFSDGGSWFEPDAALAHGLELVAQGADLVDVGGES
                     TRPGARRIPADEEARRVIPVVRELSAAGVLVSVDTMRSEVAAAALEAGAVLVNDVSGG
                     LADPAILQVVADARVPFVIMHWRGPSEDMQSRATYDDVVADVRRELAARFDAVTRAGV
                     DPAQVVLDPGIGFAKTAEHNWRLLAALPALRELGRPLLVGASRKGFLGQLLADGGEAR
                     SFDGRDVATAAISALAARDGAWCVRVHEARGSRDAVEVVAAMADPTRSPGSPASAGVR
                     T"
     gene            1591..2442
                     /locus_tag="VLC50_04605"
     CDS             1591..2442
                     /locus_tag="VLC50_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020637560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HSF26780.1"
                     /translation="MHGDGDGWTTCSLGHRHWGLQGAAGLLLRWVGPDGARVLLQHRA
                     AWSHHGDTWGLPGGARDSHESPEQAALREAAEEAGLDPATVRVRGRHRDDHVGWCYDT
                     VVADVAAPATPVAQHESAALVWVPEGEVTARAVHPGFALTWPRLAAPLVTLIVDGANV
                     VGSRPDGWWHDRLGAARRLRDELAILPATVRAGPGGGWLSVGAVVLVVEGRAAALASE
                     SDSFETVRVVAAGRDEDGDDRVVSVAGQVPSPVVVTADRGLRARVPAATVAGPGWLRG
                     LLEPAGS"
     gene            complement(2467..4170)
                     /locus_tag="VLC50_04610"
     CDS             complement(2467..4170)
                     /locus_tag="VLC50_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006543876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HSF26781.1"
                     /translation="MTRSHAWVPQAAGLACLLIGLMDIASAVTKGLAVRLHELGRLLP
                     GVLTNAAAAATVATGVLLVLLAHALRRRKRRAWRAVVLLLALSVVLHVVKGLDVEEAV
                     VSLTLLLVLVLVRDEFYAAGDPRTRWRAVGVLAALLVTDVVVGQVLLLVREGSILGRP
                     SAAERLQQVLTGLVGLDGPLRFRHESSADLVAFVLLALGLLTLLVTAYLVLRPEAPVA
                     RLDAADEARLRELVAAHGGQDSLAYFALRRDKSVIFSPGGEAAIAYRVVSGVMLASGD
                     PLGDPQAWPGAIERFVAEADRHAWVPAVVGCSRAGGQVWRRETGLTVLELGDEAVIDV
                     ATFTLEGRPMRNVRQMLARVQRAGYTCQVRRVRDIAPAERALVREQAATWRGEATERG
                     FSMALGRIGDPHDGDCVLVTAHGQGRVRAFLHFVPWGADGLSLDLMRRDRQAEPGLNE
                     LLIVGALQAAAGLGVARVSLNFAAFRAALERGDRLGAGPVMRLWRRVLGLLSRWWQIE
                     SLYRFNAKFQPWWQPRFLAYPGGATLPRIVFAILQAEAFVRVPRLFDHAPAEEPALTS
                     G"
     gene            complement(4167..>4442)
                     /locus_tag="VLC50_04615"
     CDS             complement(4167..>4442)
                     /locus_tag="VLC50_04615"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014178504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I"
                     /protein_id="HSF26782.1"
                     /translation="GRITGLSKVARLVDTFARRPQVQERLTTQIADALVRLLAPRGVI
                     VVVECEHLCMSMRGVRKPGARTVTSAVRGQLRDAATRAEAMSLILGR"
BASE COUNT          616 a         1649 c         1667 g          510 t
ORIGIN      
        1 cgtcggctag tcgaccatag tcgatggttt gtgccaaatc gtccgaatcg gcggccgctc
       61 gggtgtccac gcacaactcg acatcgacga cgaactcctg tcctgcctcg cgctcgaagt
      121 cgaagacgcc gtgccgcccc cgtccccgca acccgtgcac cccgatccga tcgctcatcc
      181 gcgctggtcc tcctgctcgt cgccgtccgg ggtgtctgag gcggacatca ccggcgacga
      241 gtggtgcgcc cacagccgcc atcccgccgg cgtacgacgg tagacgttgg tggccaccgc
      301 ctttcccccc gcgaaccccg aggggctgcc gtcgggggtc tcgacgccgg tgagcacgtt
      361 ctcgttgcac accaccagcg cgacgtcgcc gaccagcgag acgccgacgt cggtgatgaa
      421 gaactggatg tacggcgtgc cggccatcac gaccgcccag gagcgcatca ccgccgagcg
      481 gccgttgacc atcgcggtgc cggggtgcac gcagcgcacg tcgtcggcgg tgtcccagat
      541 ggaacccatg aggtcgaggt cggcgccctc gatcgcttgg tagaacgccg cgttggccgc
      601 ggtgatggcc gccgcgtcgt cggcgtcgcc ggcgctgctc acgtgcggac ccctgctgag
      661 gctggcgacc cgggtgaccg ggtcgggtcg gccatcgcgg ccaccacctc gacggcgtcg
      721 cgggaccctc gagcctcgtg cacacgcacg caccacgcgc cgtcgcgggc ggccagcgca
      781 gagatcgccg cggtggcgac gtcgcgcccg tcgaaggacc gcgcttctcc cccgtcagcg
      841 agcagctggc cgagaaaccc cttgcgggaa gcaccgacga gcaacggtcg gccgagctcg
      901 cgcagtgccg gcagggccgc gagcagccgc cagttgtgct cggcggtctt ggcgaagccg
      961 atcccggggt cgagcaccac ctgggccggg tcgaccccgg cccgggtcac cgcgtcgaag
     1021 cgggcagcca gctcgcggcg cacgtcggcc accacgtcgt cgtaggtcgc gcgggactgc
     1081 atgtcctcgc tgggaccgcg ccagtgcatg atcacgaagg ggacgcgggc gtcggccacc
     1141 acctgcagga tcgccgggtc ggccaggccg ccgctgacgt cgttgacgag cacggccccc
     1201 gcctcgagcg cggccgcggc cacctcggag cgcatcgtgt cgacactgac gagtacgccg
     1261 gccgcgctca gctcgcgcac caccgggatc acccggcggg cctcctcgtc ggccgggatc
     1321 cgccgggcgc cggggcgggt ggactcacca ccgacgtcga cgagatcggc gccctgggcg
     1381 acgagctcga gcccgtgcgc gagcgcggcg tcgggctcga accagctgcc gccgtcggag
     1441 aacgagtcgg gcgtcacgtt gaccacgccc atcaccagcg cgcgcggcac gccgcgcagc
     1501 gcctcgggca ggccggtgat cgtaggcacg gcaccagcct aggcgcccgc gaaggggccg
     1561 atccccgcac cggtgtggta cacgaggggc gtgcatggag acggtgacgg ctggacgacg
     1621 tgctcgctgg ggcaccggca ctggggtctg cagggtgctg ccggcctgct gctgcgctgg
     1681 gtgggccccg acggggcgcg ggtgctcctg cagcaccggg cagcctggag ccatcacggc
     1741 gacacgtggg gtctgcccgg cggagcccgc gacagccacg agagcccgga gcaggcggcg
     1801 ctgcgtgagg ccgccgagga ggccgggctc gacccggcga ccgtgcgggt gcgtggccgt
     1861 caccgcgacg accacgtggg ctggtgctac gacacggtgg tcgccgacgt ggccgccccg
     1921 gccaccccgg tcgcccagca cgagagcgcc gcgctcgtct gggtgcccga gggcgaggtg
     1981 accgcccggg cggtccaccc cgggttcgcg ctgacctggc cccggctggc cgcgccgctg
     2041 gtgacgctga tcgtcgacgg cgccaacgtg gtcggctccc ggcccgacgg ctggtggcac
     2101 gaccggctcg gggcggcgcg gcggctgcgc gacgagctgg ccatactgcc cgcgacggtg
     2161 cgtgccggcc ccggtggagg gtggctgtcg gtcggggcgg tcgtcctggt cgtggagggg
     2221 cgggcggccg cgctcgcgag cgagtccgac tccttcgaga ccgtccgcgt ggtggccgcg
     2281 ggccgtgacg aggacggaga cgaccgggtg gtcagcgtgg ccgggcaggt gccctccccg
     2341 gtggtggtca ccgccgaccg gggtctgcgc gcgcgggtgc cggccgccac ggtggccggg
     2401 ccgggctggc tgcgcggcct gctcgagccg gcaggctcct gacccgagcc ggttgggtcg
     2461 tgcggctcaa cccgaggtca gggctggctc ctcggccgga gcgtggtcga acaggcgcgg
     2521 cacgcggacg aaggcctcgg cctgcaggat ggcgaagacg atccggggca gcgtggcccc
     2581 gccggggtag gcgaggaagc gtggctgcca ccacggctgg aacttggcgt tgaaccggta
     2641 gagggactcg atctgccacc accgagacag cagccccagc acccgccgcc acagccgcat
     2701 cacggggccg gcgccgaggc ggtcgccccg ctcgagcgcg gcccggaacg ccgcgaagtt
     2761 caacgacacc cgcgcgaccc cgaggccggc agcggcctgc agcgcgccga cgatgagcag
     2821 ctcgttgagc cccggctcgg cctgccggtc acggcgcatg aggtcgaggg agagcccgtc
     2881 cgccccccac gggacgaagt gcaggaacgc gcgcacccgg ccctggccgt gggcggtgac
     2941 gagcacgcag tcgccgtcat gggggtcgcc gatgcggccg agggccatcg agaagccccg
     3001 ctcggtggcc tcgccgcgcc aggtggcggc ctgctcgcgg acgagggccc gttcggccgg
     3061 ggcgatgtcg cgcactcgtc gtacctggca cgtgtagcca gcgcgctgca cccgcgcgag
     3121 catctgccgc acgttgcgca tgggacgacc ctcgagggtg aaggtcgcga cgtcgatgac
     3181 cgcctcgtcg cccagctcga gcaccgtcag cccggtctcc cgccgccaca cctgcccgcc
     3241 ggccctgctg caccccacca ccgccgggac ccaggcgtgg cggtcggcct ccgcgacgaa
     3301 gcgctcgatc gcgcccggcc aggcctgcgg gtcaccgagc gggtcgccgc tggccagcat
     3361 caccccggag accacccgat aggcgatcgc ggcctcgccg ccgggcgaga agatcacgct
     3421 cttgtcgcgg cgcagcgcga agtaggcgag cgagtcctgc ccaccgtggg cggcaaccag
     3481 ctcgcgcagc cgggcctcgt cggcggcgtc gagccgggcc accggcgcct cgggccgcag
     3541 caccaggtag gcggtcacga gcaaggtgag caggccgagg gcgagcagca cgaacgccac
     3601 gagatcggcc gaggactcgt ggcggaaccg cagcggcccg tcgaggccga cgaggccggt
     3661 cagcacctgc tgcagccgct cggcggcgga gggccggccg aggatcgagc cttcgcgcac
     3721 gagcagcagg acctggccga cgaccacgtc ggtgaccagc agggccgcga gcacgcccac
     3781 cgcccgccac cgggtgcgcg ggtcgccggc ggcgtagaac tcgtcgcgca ccaggaccag
     3841 caccagcagc agggtcagcg agaccaccgc ctcctcgacg tcgaggccct tgacgacgtg
     3901 cagcacgacg ctgagcgcga ggagcaggac cacggcgcgc caggcccgcc gcttgcgtcg
     3961 gcgcagcgcg tgggccagca gcacgagcag caccccggtc gcgaccgtgg ccgccgccgc
     4021 ggcgttggtc agcacgcccg gcagcagccg ccccagctcg tgcagccgca ccgctaaccc
     4081 cttggtcacc gccgaggcga tgtccatgag cccgatgagc aggcaggcca gcccggccgc
     4141 ctggggcacc caggcgtgcg agcgggtcac cggccgagga tgaggctcat cgcctcggcc
     4201 cgggtggccg cgtcgcgcag ctgaccgcgt acggcggagg tgacggtgcg ggccccgggc
     4261 ttgcgcaccc cccgcatcga catgcacagg tgctcacact cgacgacgac gatgacgccg
     4321 cgcggggcga gcaggcgcac caacgcatcg gcgatctggg tggtcaaccg ctcctgcacc
     4381 tgcggccggc gggcgaaggt gtcgacgagc cgggccacct tggacagccc ggtgatgcgt
     4441 cc
//
LOCUS       DATIBB010000120         2921 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_197832, whole genome shotgun sequence.
ACCESSION   DATIBB010000120 DATIBB010000000
VERSION     DATIBB010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2921)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2921)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2921
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_197832"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(19..789)
                     /locus_tag="VLC50_04620"
     CDS             complement(19..789)
                     /locus_tag="VLC50_04620"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HSF26783.1"
                     /translation="MVREPGSLSGEANVSAEVNGSPGLGSVRVALASLSLLVVAVPFA
                     LLLVLVEGRWAPLLRIDVATDDALHAFAVDHDTFVNLMRLLSWSGSTTVWFVVYGAIA
                     ATLLVRGRLRLATFVVVTIAGSSLLNSFAKSAVDRARPALADPVARADGWSFPSGHAQ
                     AAIVGYTVLVLLLVWVSPWPAIARWRWLIGVAAAVMILAIGFSRIALGVHYLSDVLGG
                     YLLGAAWMLALVSAFGVLSRGRRARRVRGRRGWGVGRR"
     gene            876..1907
                     /locus_tag="VLC50_04625"
     CDS             876..1907
                     /locus_tag="VLC50_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012893370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HSF26784.1"
                     /translation="MRILVTGGTGYVGSHTVAALCRAGHDVRLLARDKAAVPAALDPL
                     GVTVSDVVFGDVTDPTSVASAVDSCDAVVHAASIYSLSPRDWARIRHTNVRGTEVVLA
                     AAVAAGADPIVHVSSTSAIMPAQPGGLINADNPPTVGAPGAYIASKAGSEAVARRYQD
                     AGAPVVTTYPGGVFGPHDPHLGEGTHRLSVELRGLMPVMPNGGTHYCDVRDVAALHAR
                     VLEPGLEPRRYVVPGHYRSGADEIRTLARLTGRRLPCVAVPAGPLMASLAPLDAVLRF
                     TRRQYPGTREGAWFLSRSAHADSSAADALGIVARPYDESIADTVRWLLASGHISARQA
                     GRLDGRPVG"
     gene            1935..2909
                     /locus_tag="VLC50_04630"
     CDS             1935..2909
                     /locus_tag="VLC50_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009944803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HSF26785.1"
                     /translation="MSNLERDPQETATASTATLEPTRELLTPREVPLGGPRAMLVRRT
                     LPHRDRRTVGAWCFVDHYGPDDVSRTAGMRVPPHPHTGLQTVSWLLEGEVLHHDSVGS
                     EATIRPGELNLMTSGRGIAHSEQSPPERPPSLHGVQLWVALPDASRHQAPHFERHATL
                     PVLREPGMTATVIAGTLGGTVSPAQMYSPIVGADLALDADVTGHVPLEPAFEHAVLAM
                     SGDVQVDGVEVPVGSLLYLGCGRSDLAVTAPGPGPARALLLGGEPFEEELVMWWNFVG
                     RDHDEIAAARADWEAHRRFGEVHGYPGDRLAAPALPTSRLKPRPRSRG"
BASE COUNT          475 a         1109 c          977 g          360 t
ORIGIN      
        1 acgacgccgc cttcgacctc accgacgacc aaccccccaa ccccgccgac cacgaacccg
       61 ccgagcccgc cgacctcgac tgagcacgcc gaacgcggag accaacgcca gcatccaggc
      121 cgctcccagc aggtagccgc ccagcacgtc ggagaggtag tgcacgccga gagcgatccg
      181 cgagaacccg atggcgagga tcatgaccgc ggcagcgaca ccgatcagcc accgccaccg
      241 ggcgatggcc ggccacgggg acacccatac gagcaaaagg accagcacgg tgtatcccac
      301 gatcgcggcc tgggcgtgcc cgctcggaaa gctccaccca tcggcacggg cgaccgggtc
      361 ggccagcgcc ggtcgcgccc ggtcaacggc cgacttggcg aaagagttga gcagcgagga
      421 cccggcgatg gtgacgacga cgaacgtcgc cagccgcaga cggcctctga ccagcagggt
      481 tgcagcgatg gcgccgtaca cgacgaacca caccgtggtc gaacccgacc agctcagcag
      541 acgcatgagg ttcacaaacg tgtcgtgatc aacggcgaag gcatgcagtg cgtcatcggt
      601 cgcgacatcg atccgaagca gcggagccca ccggccttcg accagcacca gcagcaaggc
      661 gaacggcacc gccacgacca gcagcgacag cgacgccagc gccacccgga ccgagcccaa
      721 ccccggagag ccattgacct cggcagacac gttggcctca ccagagagtg agcccggctc
      781 gcggaccatg ccccaagcat cgcccgcatg accggccgcc cgcgtgccgg ggcgaacgtc
      841 tcgccgtacg gcagattcgg gtccacgctg ggcgcgtgcg gatcctcgtc acgggcggca
      901 ctggctacgt cggcagccac acggtggcgg cgttgtgccg cgccgggcac gacgtacggc
      961 tgctcgcccg tgacaaggcg gcggtcccgg cggcgctgga cccgctcggc gtcaccgtgt
     1021 ccgacgtcgt ctttggcgat gtcacggatc cgacatcggt cgcgagcgct gtcgactcgt
     1081 gcgatgccgt cgtgcatgcc gcgtcgatct actcgctcag cccgcgggac tgggcccgga
     1141 taaggcacac caacgtgcgc ggcaccgagg tcgtgctcgc cgcggccgtt gcggccggcg
     1201 ccgacccgat cgtccatgtc tctagtacct cggcgatcat gccggcccaa ccgggcggac
     1261 tgatcaacgc cgacaaccca cctaccgtcg gagcccccgg cgcctacatc gcgtcaaagg
     1321 ccggctcgga agccgtcgcg cgccgctacc aagacgccgg cgccccggtg gtgacgacgt
     1381 acccaggcgg ggtcttcggg ccgcacgacc cgcacctggg cgaggggaca caccggctct
     1441 ctgtagaact gcgcggcctc atgccggtca tgccgaacgg cggcacgcac tactgcgacg
     1501 tacgcgacgt ggcagcgttg cacgcccggg tgctggagcc gggtctcgaa ccacgacgct
     1561 acgtcgtacc gggccactac cggtcggggg cagacgagat ccgcacgcta gcccgcctca
     1621 caggtcgacg gctgccctgt gtcgccgtac ctgccggccc gttgatggcc tcgctggccc
     1681 cgctcgacgc cgtcctgcgc ttcacccgcc gccaatatcc cggcacccgt gagggcgcat
     1741 ggttcctgtc tcgaagcgcc cacgctgaca gcagcgccgc agacgcgttg gggatcgtgg
     1801 cgcgcccgta cgacgagtcg atcgccgaca ccgtccgctg gctactggcc agcgggcaca
     1861 tcagcgcacg ccaggccggc cgactcgacg gtcggcccgt gggctgacag gaacgcccgc
     1921 cgtacagtca aggcatgagc aacctcgagc gcgacccgca ggagaccgcg acggcgtcca
     1981 ccgccacgct cgagcccacg cgtgagctgc tcactccccg cgaggtgccg ctcggcggtc
     2041 cccgggcgat gctcgtacga cggacgctgc cccaccgtga ccgtcgcacg gtcggcgcgt
     2101 ggtgcttcgt cgaccactac gggcccgacg acgtcagtcg cacagccggc atgcgcgtgc
     2161 cgccgcatcc gcacaccggg ttgcagaccg tcagctggct gctcgagggc gaggtgctcc
     2221 accacgacag cgtcggcagc gaggcgacga tccggccggg cgagctcaac ctcatgacca
     2281 gcggccgcgg gatcgcccac tccgagcaga gcccgcctga gcgccctccg agcctgcacg
     2341 gcgtgcagct gtgggtcgcg ctgccggacg cctctcgtca ccaggcgccg cacttcgagc
     2401 gccacgccac gctgccggtg ctgcgcgaac ccgggatgac cgcgacggtg atcgccggca
     2461 cgctgggcgg caccgtctcg ccggcccaga tgtactcccc catcgtcggc gccgacctcg
     2521 ccctcgacgc cgacgtgacc gggcacgtgc cgctggagcc ggcgttcgag cacgcggtgc
     2581 tggccatgtc gggcgacgtg caggtcgacg gagtcgaggt gccggtcggg tcgctgctct
     2641 acctcggctg cggccggtcc gacctggcgg tcacggcacc cggccccggt cccgcgcggg
     2701 cgttgctgct cggcggcgag ccgttcgagg aagagctggt catgtggtgg aacttcgtcg
     2761 gccgcgacca cgacgagatc gccgcggcac gcgccgactg ggaggcgcat cgccggttcg
     2821 gagaggttca cggctatccc ggcgaccggc tggccgcccc cgcactgccc accagccggc
     2881 tcaagccgcg cccccgctcc cgtgggtgag cgactgatcg a
//
LOCUS       DATIBB010000121         2185 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_332464, whole genome shotgun sequence.
ACCESSION   DATIBB010000121 DATIBB010000000
VERSION     DATIBB010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2185
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_332464"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..850
                     /locus_tag="VLC50_04635"
     CDS             <1..850
                     /locus_tag="VLC50_04635"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019633660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HSF26786.1"
                     /translation="VVAAMTAMAERIEIVSAERVSDELVKLMLAADPKPGLRLLVDTG
                     LAEHVLPELPRLRLEIDEHHRHKDVYEHTLTVLDQAIALETEGPDLVLRLAALLHDIG
                     KPATRRFEPGGGVSFHHHEVVGAKLTRKRLRALRLPKDVVDDVSRLVELHLRFHGYGT
                     GEWTDSAVRRYVRDAGPLLDRLHALTRSDCTTRNKRKAAALARTYDELEARIARLGEE
                     EELAAIRPDLDGNEIMAELGVPAGPLVGRAYRHLLECRLDRGPLGREAALDELRRWWA
                     DQPESR"
     gene            complement(899..2173)
                     /locus_tag="VLC50_04640"
     CDS             complement(899..2173)
                     /locus_tag="VLC50_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020574638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSF26787.1"
                     /translation="MSYVADLRILLQGRDFRRLFGTRLTSQLADGWFQVALAGLFFFS
                     PERATTAHGVAGAFAVLLLPYSLLGPFVGVLLDRWRRRQVLVRSNLLRAGMVLGVAAI
                     IVAELRDLVLAVAVLATLAVNRFYLAALSAGLPHVVSREELVLANAVTPTSGTLAFVT
                     GLGTGVVARAVLGDDTRVLLLASVGYLASAWIAARMHPDRLGPHGGLERVALRVAAGQ
                     VVSGLIEGARHVWATRPARNALGAMTTMRFAYGLSAIAVVLLMRNYFNDPGRPDDGLA
                     DLALVFLASGLGFFAAALLTPGATRVLGMRRWMVVCLGAAGLVNATLVPWLERGWVIS
                     ISLLVGLAAQGVKICVDTIVQETIDDGFRGRVFSFYDVLFNVAFVSAAAIAALVVPSD
                     GSARWLYGAIGGVYAAAALAYAAVTPRPTPLP"
BASE COUNT          354 a          804 c          793 g          234 t
ORIGIN      
        1 cgtcgtggcc gcgatgaccg cgatggccga gcggatcgag atcgtctcgg ccgagcgggt
       61 cagcgacgag ctggtcaagc tgatgctggc ggccgacccc aagcccgggc tgcggctgct
      121 cgtcgacacc gggctggccg agcacgtgct gcccgagctg ccccggttgc ggctggagat
      181 cgacgagcac caccggcaca aggacgtcta cgagcacacc ctgaccgtgc tcgaccaggc
      241 gatcgccctg gagaccgaag gacctgacct ggtgctgcgg ctggccgcgc tgctgcatga
      301 catcggcaag ccggcgaccc gtcggttcga gcccggcggc ggggtgtcgt tccaccacca
      361 cgaggtggtc ggcgccaagc tgacccgcaa gcggctgcgc gcgctgcgcc tgcccaagga
      421 cgtcgtggac gacgtgtccc ggctggtcga gctgcacctg cgcttccacg gctacggcac
      481 cggcgagtgg accgacagcg ccgtacgccg ctatgtccgt gacgccggcc cgctgctcga
      541 ccggctgcac gcgctgaccc ggtcggactg cacgacccgc aacaagcgca aggcggcggc
      601 gctggcccgc acctatgacg agctcgaggc ccgcatcgcc cggctcggcg aggaggagga
      661 gctggccgcg atccggcccg acctcgacgg caacgagatc atggccgagc tgggcgtgcc
      721 ggccgggccg ctggtgggcc gggcctaccg gcacctgctc gagtgccgcc tcgaccgcgg
      781 cccgctgggg cgcgaggcgg cgctggacga gctgcgccgc tggtgggccg accagcccga
      841 gtcccgctga gcgccccacc cctgcactga tcatgcgcgt ctggcggcgc ggaaagggtc
      901 agggcagggg ggtgggacga ggggtgacgg cggcataggc cagggcggcg gccgcgtaga
      961 ccccgccgat cgcgccgtag agccaccggg ccgagccatc agacggtacg acgagcgcgg
     1021 cgatcgccgc cgccgagacg aaggcgacgt tgaacagcac gtcgtagaac gagaacaccc
     1081 gcccacggaa gccgtcgtcg atggtctcct gcacgatcgt gtcgacgcag atcttgacgc
     1141 cctgcgcggc cagaccgacc agcaggctga tcgagatcac ccagccgcgt tccagccacg
     1201 gcaccagggt cgcgttgacg agcccggccg ccccgaggca gacgaccatc cagcgccgca
     1261 tgcccagcac ccgggtcgcc cccggggtca gcagcgccgc ggcgaagaac ccgagcccgg
     1321 aggccaggaa gaccagcgcc aggtcggcca gcccgtcgtc ggggcggcct gggtcgttga
     1381 agtagttgcg catcagcagg accaccgcga tggcgctcaa cccgtaggcg aagcgcatcg
     1441 tggtcatcgc gccgagtgcg ttgcgggccg gcctggtcgc ccacacgtgc cgggcgccct
     1501 cgatcagccc gctcaccacc tgcccggccg ccacgcgcag cgcgactcgc tcgagcccgc
     1561 cgtgcggtcc cagccggtcg gggtgcatcc gcgccgcgat ccacgccgag gcgaggtagc
     1621 cgaccgaggc cagcaggagg acccgggtgt cgtcgcccag cacggcgcgc gcgacgaccc
     1681 cggtgcctag cccggtgacg aaggccagcg tcccgctcgt cggggtcacc gcgttggcca
     1741 gcaccagctc ctcgcgcgag accacgtgcg gcagcccggc cgagagcgcg gcgaggtaga
     1801 accggttgac cgccaacgtc gctagcaccg cgacggccag cacgaggtcg cgcagctcgg
     1861 ccacgatgat cgcggcgacg ccgaggacca tcccggcgcg cagcaggttg gagcgcacca
     1921 gcacctgccg gcgccgccag cggtcgagca gcaccccgac aaacggtccg agcagcgaat
     1981 agggcagcag cagtacggcg aacgcgccgg ccaccccgtg cgcggtggtc gcgcgctcgg
     2041 gcgagaagaa gaacagcccg gccagcgcga cctggaacca gccgtcggcc agctgcgagg
     2101 tcagccgggt gccgaacagc cgccggaagt cacgcccctg cagcaggatg cgcaggtccg
     2161 cgacgtagga caccggggca gccta
//
LOCUS       DATIBB010000122         6885 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_332810, whole genome shotgun sequence.
ACCESSION   DATIBB010000122 DATIBB010000000
VERSION     DATIBB010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 6885)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6885)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6885
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_332810"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..247
                     /locus_tag="VLC50_04645"
     CDS             <1..247
                     /locus_tag="VLC50_04645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HSF26788.1"
                     /translation="KAVSGIREFVHDGQEGLLAGTDVEMVDALVRLCTDDELRARIAA
                     HNRDAEPDMVWPRVLKRTEEEYLRAIAIMARHRAAGR"
     gene            307..1182
                     /gene="dapF"
                     /locus_tag="VLC50_04650"
     CDS             307..1182
                     /gene="dapF"
                     /locus_tag="VLC50_04650"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014675444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HSF26789.1"
                     /translation="MSLDPGTVEFVKGHGTANDFVVLPDPDGRLALTPARVRALCDRR
                     AGLGGDGVLRVVRVEAAGPDAAAHRGAAEWFMDYRNADGSVAEMCGNGVRVFARYLVE
                     AGLAARGPLTVAPRGGRKHVRFEDSGEITVDMGPATLVEAEPTVSVDGRSWAALAVFA
                     PNPHAVVFVDDLAQAGTLLAAPHVEAPSIYPDGVNVEFVVDRGPTHVAMRVHERGVGE
                     TRSCGTGACAVAVAARRRAGWAPADETDRVWTVDVPGGRLRVTERADGHLELTGPAVL
                     VSSGTLEPTWWTAHA"
     gene            1179..1991
                     /locus_tag="VLC50_04655"
     CDS             1179..1991
                     /locus_tag="VLC50_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020541774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF26790.1"
                     /translation="MSAEPMPTSTDASTPSAPAWGPAAGAARLVRALQGPLERLGSGF
                     MVSPEAAALRDSLGLHGWEVYAGGRFGVLGDVEADVVTAAAVFFPAERIRTLWKAARA
                     AGAPHELAVHYAGAAHDWARRRLAGFAGAERLAALLEPLVTQAPEAGAPLFAGWRALP
                     LPDDAPARAVQLLHVLREHRGAMHAVAVLASGLSPLQAILAGPEGEEGARYFRWPAPF
                     PVVDADVRAAHRWAEERTDLLEAPVWAQLGDEADECAALLRAADAHARSTAD"
     gene            2048..3475
                     /gene="hflX"
                     /locus_tag="VLC50_04660"
     CDS             2048..3475
                     /gene="hflX"
                     /locus_tag="VLC50_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016470241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HSF26791.1"
                     /translation="MSTPNPDPRLDGEPTPAYDGDQLDLADRVALRRVAGLSTELADV
                     TEVEYRQLRLERVVLIGVWTEGTVADAEVSMAELAALAETAGSVVLDALIQRRDRPDP
                     ATYVGSGKARELRDIVLATGADTVICDGELTPGQLVHLEDVVKVKVVDRTALILDIFA
                     QHAKSREGKAQVSLAQMQYMLPRLRGWGQSMSRQAGGRVAGGGGIGSRGPGETKIETD
                     RRRIRDRMAKLRREITHMKKARDTKRAGRTRNAVPAVVIAGYTNAGKSSLLNRLTGAG
                     VLVENALFATLDPTVRRAETPAGRPYTLADTVGFVRHLPHQLVEAFRSTLEEVAAADL
                     VLHVVDGSHPDPEGQLGAVRQVLLEVGARDVPEIVVVNKADAADPLVLDRLRRREPHS
                     VIVSARTGQGLDELRAAIERDLPRPAVEVDVVVPYARGELVSRVHEHGKVLAEEHTPD
                     GTRLRALVPESLAAELVGFPVGASR"
     gene            3574..5595
                     /locus_tag="VLC50_04665"
     CDS             3574..5595
                     /locus_tag="VLC50_04665"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019877866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HSF26792.1"
                     /translation="MAAAVRAIGGRRRDGQEQMAVAVAEALGSGEHLLVQAGTGTGKS
                     LAYLVPAVVHAVTTGETVVVSTATIALQQQVADRDLPAVAAALAPLLPRPVRFATLKG
                     RRNYLCVHRVDGGLPADDDDAALFAAAPPSGLGREVLRLRQWAEATDTGDRDALDPGV
                     SERAWGQVSVSSRECLGASRCPAADRCFAEAARERARGADVVVTNHALLAIDAVEGLP
                     VLPEHALVVVDEAHELVDRVTSVATGELSAGMVERAARRAARIVHDDGAGPIEALAGA
                     GVSLRAVLDALPEGRLDASPEPLTVSLAALRDAARTVLTAITAERGADDGAKTLARAA
                     VDAVHEVAERLAAGSEHDVVWVHRDERRGPMLYVAPLSVQGLLRTTLFEQATVVLTSA
                     TLSLGGSFDLVARGLGLGDRSADEAAAGELADAPTPDSPRPDAPGLARWRGLDVGSPI
                     DYPRQGILYVARHLPRPGRDGIAAAALDELAELVTAAGGRTLGLFSSMRAATAAAAAM
                     RERLDVPVLCQGEDRTAALVRAFAADGPTCLFGTLSLWQGVDVPGSACQLVVIDRIPF
                     PRPDDPLASARARAVEAAGGNGFMTVSAAHAALRLAQGMGRLIRSGEDRGVVAVLDPR
                     LATARYAGFLRASLPGFWATTDSAQVRRSLAALAALAASAPPPRAVPDV"
     gene            complement(5592..6077)
                     /locus_tag="VLC50_04670"
     CDS             complement(5592..6077)
                     /locus_tag="VLC50_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013130387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HSF26793.1"
                     /translation="MTSSEELLSDERLTALGLLEETRAGLHERFAEQLGTHGLAELDF
                     EVLLRLGRSPDTRLRMSDLASQVNMSASGLTRVIDRLEAADLVARMTCREDRRGTWAT
                     VTEAGIARLHAALPDHLQLIQKWYVGVLEPDELTELTRLLRKLRDVVRPQAVAGASPG
                     H"
     gene            6189..6761
                     /locus_tag="VLC50_04675"
     CDS             6189..6761
                     /locus_tag="VLC50_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HSF26794.1"
                     /translation="MSTTTTRTHNGVEIPAAGTFVIDKSHAIVGFVARHLMVAKVRGR
                     FTELEGTITIAEDPAESSAQVVLQTASVTTNDEGRDAHVKSADFLDVETYPTISFAST
                     SLQERGDGRYVLAGDLTIKDVTKPVELDVEFEGRIVDPWGGERIGFTASTEVEREDWG
                     LTWNVALESGGVLVSKKIKLEVEVEAVRQA"
BASE COUNT          853 a         2552 c         2584 g          896 t
ORIGIN      
        1 caaggcggtc agcggcatcc gcgagttcgt ccacgacggg caggaggggc tgctggccgg
       61 caccgatgtc gagatggttg acgcgcttgt gcgcttgtgc accgacgacg agctgcgggc
      121 acgcatcgcc gcgcacaacc gcgacgccga gccggacatg gtctggccgc gggtgctcaa
      181 acgcactgag gaggaatacc tgcgcgccat cgcgatcatg gcgcggcacc gggccgcggg
      241 tcgctgaccg tgcgctgacc gtgcgctgac cgtgcgctga gccggccagg gcctagcatc
      301 gggcgcgtga gtctcgatcc gggcaccgtg gagttcgtca agggccacgg caccgccaac
      361 gacttcgtcg tgctgcccga ccccgacggg cggctggcgc tgacccccgc ccgggtgcgc
      421 gccctgtgtg accggcgcgc cgggctgggc ggcgacgggg tgctgcgcgt cgtacgcgtc
      481 gaggcggccg gcccggacgc ggcggcgcac cgcggtgccg ccgagtggtt catggactac
      541 cgcaacgccg acgggtcggt ggccgagatg tgcggcaacg gggtgcgggt cttcgcccgc
      601 tacctcgtcg aggcgggcct ggccgcgcgc ggcccgttga ccgtcgcccc ccggggcggg
      661 cgcaagcacg tgcggttcga ggactccggt gagatcaccg tcgacatggg cccggccacc
      721 ctcgtcgagg ccgagccgac ggtctcggtc gacggccgca gctgggccgc cctggccgtg
      781 ttcgcgccca acccgcacgc ggtcgtcttc gtcgacgacc tcgcccaggc gggcaccctg
      841 ctcgccgccc cccacgtcga ggctccgagc atctaccccg acggggtcaa cgtggagttc
      901 gtcgtcgacc gcgggccgac gcacgtggcc atgcgagtcc acgagcgcgg ggtgggcgag
      961 acccgctcct gcggcaccgg cgcctgcgcg gtggccgtgg ccgcccgccg gcgggccggg
     1021 tgggcgccgg ccgacgagac cgaccgggtc tggaccgtcg acgtgcccgg cggccggctt
     1081 cgggtgaccg aacgcgccga cggccacctc gagctgaccg ggcccgcggt gctggtctcc
     1141 tccggcacgc tcgagccgac ctggtggacg gcccacgcgt gagcgccgag ccgatgccca
     1201 cgtcgaccga cgcctcgacg ccgtccgcgc cggcctgggg gccggccgcc ggggcagccc
     1261 ggctggtccg cgccctgcag ggcccgctcg aacgcctcgg cagcgggttc atggtctctc
     1321 ccgaggcggc ggccctgcgc gactcgctgg gcctgcacgg ctgggaggtc tacgccggcg
     1381 gccggttcgg cgtgctcggt gacgtcgagg ccgacgtggt gaccgcggcc gcggtgttct
     1441 tcccggccga gcggatccga accctctgga aggccgcgcg cgcggccggc gcgccgcacg
     1501 agctggccgt ccactacgcc ggcgccgcgc acgactgggc gcgccgccgc ctggccggtt
     1561 tcgccggggc cgagcggctg gctgcgctgc tggagccgct ggtcacacag gccccggagg
     1621 ccggcgcccc gctgttcgcc ggctggcggg ctctgccgct gcccgacgac gcgccggctc
     1681 gcgccgtaca gctgctccat gtcctgcgcg agcaccgcgg tgcgatgcat gccgtggccg
     1741 tgctcgcgag cgggctgagc ccgctgcagg ccatcctcgc cggcccggag ggggaggagg
     1801 gggcccgcta ttttcgctgg cctgcaccct tccccgtggt ggatgctgac gtccgcgccg
     1861 cacaccggtg ggccgaggaa cgcaccgacc tgctcgaggc gccggtctgg gcgcagctcg
     1921 gtgacgaggc cgacgagtgc gctgcgctgc tgcgcgccgc cgacgcccac gcgcggagca
     1981 cggcagactg accacggcag acgcagtcag ggacgatccc cgacccgtga ggtgttgagc
     2041 ccaacgtatg agcactccca atcctgaccc ccgcctcgac ggtgagccga ccccggccta
     2101 tgacggcgac cagctcgacc tcgccgaccg ggtcgcgctg cgccgcgtgg ccggcctgtc
     2161 caccgagctg gccgacgtca ccgaggtcga gtatcgccag ctgcgcctcg agcgggtggt
     2221 gctcatcggc gtctggaccg agggcaccgt ggccgacgcc gaggtctcga tggccgagct
     2281 ggccgcgctg gccgagactg ccggctcggt cgtgctcgac gcgttgatcc agcggcgcga
     2341 ccggcccgac ccggccacct acgtgggctc cggcaaggcc cgcgagctgc gcgacatcgt
     2401 gctggccacc ggcgccgaca ccgtcatctg cgacggcgag ctgaccccgg gccagctggt
     2461 gcacctcgag gacgtcgtca aggtcaaggt cgtcgaccgc accgcgctga tcctcgacat
     2521 cttcgcccag cacgccaaga gccgcgaggg caaggcgcag gtctcgctgg cccagatgca
     2581 gtacatgctg ccgcggctgc gcggctgggg tcagtcgatg tcccggcagg ccggcggccg
     2641 ggtggccggc ggtggcggta tcggctcccg cggccccggt gagaccaaga tcgagaccga
     2701 tcggcggcgc atccgcgacc ggatggccaa gctgcgccgc gagatcactc acatgaagaa
     2761 ggcccgcgac accaagcggg ccgggcgcac ccgcaacgcg gtgccggccg tggtcatcgc
     2821 cggctacacc aacgccggca agtcgagcct gctcaaccgg ctcaccgggg ccggcgtgct
     2881 ggtcgagaac gcgctgttcg ccaccctcga cccgaccgtg cgccgggccg agacgcccgc
     2941 cgggcggccc tacacgctgg ccgacaccgt gggcttcgtt cgtcacctgc cgcaccagct
     3001 cgtcgaggcg ttccgctcga cgctcgagga ggtggccgcc gccgacctgg tgctgcacgt
     3061 cgtcgacggg tcgcaccccg acccggaggg tcagctggga gccgtccggc aggtgctcct
     3121 cgaggtcggt gcgcgcgacg tgcccgagat cgtggtcgtc aacaaggccg acgccgccga
     3181 cccgctggtc ctcgaccggc tgcgccggcg cgagccccac agcgtcatcg tgtccgcccg
     3241 cacggggcag ggtctcgacg agctgcgcgc ggcgatcgag cgcgacctgc cgcgcccggc
     3301 ggtcgaggtc gacgtcgtgg tgccctacgc gcgcggcgag ctggtctccc gggtccacga
     3361 gcacggcaag gtcctggccg aggagcacac ccccgacggc acccggctgc gggccctggt
     3421 gcccgagtcg ttggccgccg agctggtggg gttcccggtc ggcgcctccc ggtgagcgcg
     3481 caaccggcca ccgacctggc cgacgccggc tcggccgacg ccggtctggt cgacgaaggc
     3541 caggcccccg aaggtcctgg cgtccgcgcg gcgctggccg ccgccgtacg cgcgatcggt
     3601 ggccgccgcc gcgacgggca ggagcagatg gcggtggcgg tcgccgaggc gctgggctcg
     3661 ggcgagcacc tgctcgtgca ggccggcacc ggcaccggca agtcgctggc ctacctggtc
     3721 cccgcggttg tgcacgcggt gaccaccggc gagaccgttg tggtgagcac ggccacgatc
     3781 gcgctgcagc agcaggtcgc cgaccgcgac ctgccggcgg tggccgcggc gctggcaccg
     3841 ctgctgcccc gcccggtccg gttcgccacc ctcaaggggc ggcgcaacta cctgtgcgtg
     3901 caccgggtcg acggcgggct gcccgccgac gacgacgatg cagcgctgtt cgcggcggcg
     3961 ccgcccagcg ggctgggtcg ggaggtgctc cggctgcgcc agtgggccga ggcgaccgac
     4021 accggcgacc gcgacgcgct cgaccccggc gtgtccgagc gggcctgggg ccaggtctcg
     4081 gtcagctcgc gggagtgcct gggagccagt cgttgcccgg ccgccgaccg gtgcttcgcc
     4141 gaggccgcac gtgagcgggc ccgcggggcc gacgtcgtcg tgaccaacca cgccctgctg
     4201 gccatcgacg cggtcgaggg gctgccggtg ctgcccgagc acgcgctcgt ggtcgtcgac
     4261 gaggcgcacg agctggtcga ccgcgtcacc agcgtggcca ccggcgagct gtcggccggc
     4321 atggtcgagc gggcggcccg ccgggccgcc cggatcgtgc acgacgacgg cgccggcccg
     4381 atcgaggcgc tcgcgggggc gggcgtgagc ctgcgcgcgg tgctcgacgc gctgcccgag
     4441 ggccggcttg acgcatcacc cgaaccgctc acggtgtcgc tggccgcgct gcgcgacgcg
     4501 gcccggacgg tgctcaccgc gatcactgcc gagcgcggtg ccgacgacgg ggccaagacg
     4561 ctggcccggg ccgcggtcga cgctgtgcac gaggtggccg agcggttggc cgccggcagc
     4621 gagcacgacg tggtctgggt gcatcgcgac gagcgacggg ggccgatgct ctacgtcgcc
     4681 ccgctgtcgg tgcagggcct gctgcgcacc acgctgttcg agcaggcgac cgtcgtactc
     4741 acctcggcca cgctctcgct cggcgggtcc ttcgacctgg tggcccgtgg gctcggcctg
     4801 ggtgaccggt ccgccgacga ggcggcggcc ggcgagctcg ccgacgcccc cacgcccgac
     4861 tcgcccaggc ccgatgcccc cgggctggca cggtggcgcg ggctcgacgt cggctcgccg
     4921 atcgactacc cgcgccaggg catcctctac gtcgcgcgcc acctgccccg acccggccgg
     4981 gacggcatcg ccgcggccgc cctcgacgag ctggccgagc tggtcaccgc ggcgggtggg
     5041 cgcacgctcg ggctgttctc ctcgatgcgc gccgcgaccg cggcggccgc ggcgatgcgc
     5101 gagcgactcg acgtgccggt gctgtgccag ggagaggacc gtacggcggc gctcgtgcgg
     5161 gccttcgccg ccgacgggcc gacctgcctg ttcggcacgc tctcgctctg gcagggggtc
     5221 gacgtgccgg ggtcggcgtg ccagctggtg gtcatcgacc ggatcccgtt cccgcgcccc
     5281 gacgacccgc tcgcctcggc ccgcgcgcgg gcggtcgagg cggccggggg caacgggttc
     5341 atgaccgtct ccgccgcgca cgcggcgctg cggctggccc agggcatggg ccggctgatc
     5401 cgctccggcg aggaccgcgg ggtggtggcg gtgctcgacc cgcggctggc gaccgcgcgc
     5461 tatgccgggt tcctgcgcgc gtcgctgcct ggcttctggg ccaccaccga ctcggcccag
     5521 gtgcgccgct cgctggcggc gctggcggcg ctcgccgcgt cggcgccgcc gccccgagcg
     5581 gtgcccgatg tctagtgccc gggcgaggct ccggccaccg cctggggtcg gaccacgtct
     5641 cggagcttgc gcagcagccg ggtgagctcg gtgagctcgt ctggctcgag gacgccgacg
     5701 taccacttct ggatcagctg cagatggtcg gggagcgcgg cgtgcaggcg ggcgatcccg
     5761 gcctcggtga cggtggccca ggtgccgcga cggtcttccc ggcaggtcat ccgtgccacc
     5821 aggtcggccg cctcgagccg gtcgatgacc cgggtcagcc cgctggcgga catgttgacc
     5881 tggctggcga gatcggacat gcgcagccgg gtgtcagggg agcggcccaa ccgcagcagc
     5941 acctcgaagt cgagctcggc cagcccatgg gtgcccagct gctcggcgaa gcgctcgtgc
     6001 aggccggcac gggtctcctc gagcaggccg agcgcggtca gccgctcgtc ggacaggagc
     6061 tcctcgctgc tggtcatggc cccaccctag cgtcgatagt tgcgttaggg aaatattcgc
     6121 ggtacaacta ttgctgaagc aagcaagccg cgagcgcccc gcccgcacca ttttgctcgg
     6181 aggaacagat gtccacgacc accacacgca cccacaacgg tgtagagatc cccgcagccg
     6241 gcacattcgt gatcgacaag tcgcacgcga tcgtcggctt cgtcgcccgc cacctcatgg
     6301 tcgccaaggt gcgcggccgg ttcactgagc tcgagggcac gatcacgatc gccgaggacc
     6361 ccgccgagtc gagtgcgcag gtggtcttgc agaccgccag cgtcaccacc aacgacgagg
     6421 gccgcgacgc ccacgtcaag agcgccgatt tcctcgacgt cgagacctac ccgacgatct
     6481 cgttcgcctc gacgagcctg caggagcgcg gcgacggtcg ttatgtcctg gccggcgacc
     6541 tgacgatcaa ggacgtcacc aagccggtcg agctcgacgt cgagttcgag ggccgcatcg
     6601 tcgacccgtg gggcggcgag cgcatcggct tcaccgcctc gaccgaggtc gagcgcgagg
     6661 actggggtct gacctggaac gtcgcgctgg agagcggcgg cgtcctggtg agcaagaaga
     6721 tcaagctcga ggttgaggtc gaggcggtcc gccaggcctg agcctgcagc ctgcacgcgc
     6781 ctggcccagc cagcacgcag ggccgtcacc gtgcctgcac ggtggcggcc cttgtgcgtc
     6841 gaccggtcgg gaggcctaga tccggcggag cacggcggtg acgcg
//
LOCUS       DATIBB010000123         2792 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_333817, whole genome shotgun sequence.
ACCESSION   DATIBB010000123 DATIBB010000000
VERSION     DATIBB010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2792
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_333817"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..648
                     /locus_tag="VLC50_04680"
     CDS             <1..648
                     /locus_tag="VLC50_04680"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HSF26795.1"
                     /translation="RVTSEVVRAPVAEALLKTASSDTALLVVGSRGRGGFRSLLLGST
                     SMEVAAKSPCPVAIVRQGEMPSQRHGRVVVGVDGSELSKAAVAFAMVEASRRGAALEA
                     ITAWNRVEALPPGLIAAGVVEAMPTTEEIALGLSQTLAGWRERCPDVQVVQRVVVGHP
                     GHVLTEASKSADLVVVGSRGLGNAASLLIGSTSRNVLHHAVCPVVVVPSAIDPDD"
     gene            complement(666..2360)
                     /gene="paaN"
                     /locus_tag="VLC50_04685"
     CDS             complement(666..2360)
                     /gene="paaN"
                     /locus_tag="VLC50_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011757919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation protein PaaN"
                     /protein_id="HSF26796.1"
                     /translation="MTSAQQCFDRHRPMLETAVAATRIRGYYSAFPESPSPRVYGEHA
                     AAEGKAAFDSWLGREVELVVPGSAGTVATEQSPYGVMLGTRYPRVSTAGVDALLGAAQ
                     AAMPAWRDLGVEGRVGVCLEILARLHARIFELAHAVMHTSGQAFVMAFQAGGAHALDR
                     GLESVAYAYDEMTRHPATAAWEKPAGRGEPLRMEKTFTVVGRGVSLVIGCTTFPTWNS
                     WPGLFASLATGNAVVVKPHPRAVLPLAITVQVAQQVLAEVGLDPALVTLAAEDPADKL
                     AATLATRPEVRLVDFTGSSAFGGWLEQHATQALVFTEKSGVNAVIVDSTDDLTGLCAN
                     LAFSFTLYAGQMCTTPQNVFVPAEGIDTDQGHKSFAEVGAGLAAAIEALVADDAKAVA
                     LLGAIVNDDVLARAEAAGSSGQLVLASRPVAHPEFPDAVVRTPALVALDGADAAHQVL
                     YARECFGPVSFLIRTGSTDESVELFRRTVREHGAMTASVYSTSPAVLEAAREAALDAG
                     VALSENLTGGVYVNQSAAFSDFHGTGANPAANSAYTDTAYVAGRFRTVQSRRHVLA"
     gene            2447..>2792
                     /locus_tag="VLC50_04690"
     CDS             2447..>2792
                     /locus_tag="VLC50_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HSF26797.1"
                     /translation="MADDDTVRRDVVGVVGAGTMGAGIAQVAAMAGHETRLVDAAPGA
                     AAHAVETARARIARLAEKGRLTAADAAAASARLRAVDDLAALAGAALVVEAIVENLAA
                     KQALFVELEARLG"
BASE COUNT          375 a          979 c         1071 g          367 t
ORIGIN      
        1 cgcgtcacca gcgaggtcgt cagggcgccg gtcgccgagg cgctgctcaa gacggccagc
       61 agcgacaccg ccctgctcgt ggtgggctcc cggggccgcg gcgggttccg ctcgctgctc
      121 ctggggtcga cctccatgga ggtggcggcc aagtcaccct gcccggttgc catcgtccgg
      181 cagggcgaga tgccctcgca gcgccatggg cgggtcgtcg tcggcgtgga cggctccgag
      241 ctgtccaagg ctgcggtcgc cttcgcgatg gtcgaggcct cccgccgcgg ggccgcgctc
      301 gaggcgatca ccgcatggaa ccgggtggag gcgctgccgc ccgggctgat cgccgccggc
      361 gtcgtcgagg cgatgccgac caccgaggag atcgcgctgg ggctgtccca gaccctggcc
      421 ggctggcgcg agcgctgccc cgacgtgcag gtcgtgcagc gggtggtggt cggccacccc
      481 ggtcacgtgc tgacggaggc gtccaagtcg gccgacctgg tcgtcgtcgg ctcgcggggg
      541 cttggcaacg cggcgagcct gctcatcggc tcgacgagcc gcaacgtgct gcaccacgcg
      601 gtgtgcccgg tggtcgtcgt accgtcggcg atcgaccccg acgactaggc gccgcggcac
      661 tcgtctcagg cgagcacgtg ccgccgtgac tggacggtgc ggaaccggcc ggcgacgtat
      721 gccgtgtcgg tgtaggccga gttggccgcc gggttggccc cggtgccgtg gaagtcgctg
      781 aaggccgccg actggttgac gtagaccccg ccggtgaggt tctccgacag cgccaccccg
      841 gcgtccagcg cggcctcgcg ggccgcctcg aggacggccg gcgacgtgga gtagaccgag
      901 gcggtcatcg cgccatgctc tcggacggta cggcggaaca gctcgaccga ctcgtccgtg
      961 gaccccgtgc ggatgaggaa ggacaccggg ccgaagcact cgcgggcgta gagcacctgg
     1021 tgggccgcgt cggcgccgtc gagggcgacc agggccggcg tgcgcacgac ggcgtcggga
     1081 aactcgggat gcgcgaccgg tcgggaggcc agcacgagct ggcccgacga accggccgcc
     1141 tcggcgcggg ccagcacgtc gtcgttgacg atcgcgccga gcagtgcgac cgccttggcg
     1201 tcgtcggcga ccaaggcctc gatggcggcc gccaggcccg cgcccacctc ggcaaagctc
     1261 ttgtgcccct ggtcggtgtc gatgccctcg gcgggcacga acacgttttg cggggtggtg
     1321 cacatctggc cggcatagag ggtgaacgag aacgcgaggt tggcgcacag cccagtgagg
     1381 tcgtcggtcg agtcgacgat gaccgcgttg accccggact tctcggtgaa caccagcgcc
     1441 tgggtggcgt gctgctcgag ccagccgcca aaggcgctcg agccggtgaa gtcgacgagc
     1501 cggacctcgg ggcgggtggc cagcgtcgcg gccagcttgt cggcggggtc ctcggcggcc
     1561 agcgtgacca gcgcggggtc aagcccgacc tcggcaagca cctgctgggc cacctgcacg
     1621 gtgatcgcca gcgggagcac ggcgcgcggg tgcggcttga cgacgaccgc gttgccggtg
     1681 gccagcgagg cgaacaggcc cggccaggag ttccaggtcg ggaacgtcgt gcagccgatc
     1741 accagcgaga cgccacggcc gacgacggtg aacgtcttct ccatgcgcag cggctcgccg
     1801 cgcccggccg gcttctccca ggccgcggtg gccgggtgcc gggtcatctc gtcgtaggcg
     1861 taggcgaccg actcgaggcc gcggtcgagg gcgtgggccc cgccggcctg gaacgccatg
     1921 acgaaggcct gtccgctggt gtgcatcacc gcgtgggcca gctcgaagat ccgcgcgtgc
     1981 agccgggcga ggatctccag gcacaccccg acccggccct cgacgcccag gtcgcgccag
     2041 gccggcatcg cggcctgcgc ggcacccagc agcgcgtcca ccccggcggt gctcacccgc
     2101 ggatagcggg tgcccagcat gacgccgtac ggcgactgct cggtggccac cgtcccggca
     2161 gagcccggaa ccaccagctc gacctcacga ccgagccagg aatcgaacgc cgccttgccc
     2221 tcggcggcgg cgtgctcgcc atagacccgc ggactcggtg actccgggaa ggccgagtaa
     2281 tagccgcgga tgcgggtggc cgcgaccgcg gtctcgagca tcggtcggtg ccggtcgaag
     2341 cactgctggg cgctcgtcat cgcgggtcgt gtccttgatg gctcgggccg accagtggcg
     2401 ctggcggctg tgacaggatt gcgtccgccc tgcgaaaggc tgacacatgg cagatgacga
     2461 caccgtacgc cgcgacgtgg ttggtgtggt cggggccggc acgatggggg cggggatcgc
     2521 ccaggtcgcc gcgatggctg ggcacgagac gcgcctcgtc gacgccgcgc ccggcgcggc
     2581 cgcacacgcc gtcgagaccg cccgcgcccg tatcgcccgg ctggccgaga agggccggct
     2641 caccgccgcg gacgcggccg ccgcgagcgc ccggctgcgg gccgtcgacg acctggccgc
     2701 gctcgccggg gcggcgctgg tcgtcgaggc gatcgtggag aacctcgcag ccaagcaggc
     2761 gttgttcgtc gagctggagg cgcggctcgg cg
//
LOCUS       DATIBB010000124         2011 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_1781269, whole genome shotgun sequence.
ACCESSION   DATIBB010000124 DATIBB010000000
VERSION     DATIBB010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2011
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1781269"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..522)
                     /locus_tag="VLC50_04695"
     CDS             complement(<1..522)
                     /locus_tag="VLC50_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HSF26798.1"
                     /translation="MAEFIYTMRKARKAHGDKVILDDVTLSFLPGAKIGVVGPNGAGK
                     STVLKIMAGLDQPSNGDAFLSPGYSVGILQQEPQLDESKDVLGNVQDGVRVIKAKLDR
                     FNEIAELMATDYSDELMEEMGRLQEEIDHAGAWDLDSQLEQAMDALRCPPPDADVTVL
                     SGGERRRVALCRLL"
     gene            complement(588..1199)
                     /gene="ssb"
                     /locus_tag="VLC50_04700"
     CDS             complement(588..1199)
                     /gene="ssb"
                     /locus_tag="VLC50_04700"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HSF26799.1"
                     /translation="MNETYVTVVGNAVTGVALRVTDGGTPMATFRVANTPRRFDRERN
                     SWQDGPSSFYSVTCWRALAENAYASIKVGQPVLVTGRQRVRPWTGKDGRAGLSVEIDA
                     VALGHDLTRGQTTFEKVRRGQGGDAQAQASEVALAELADQPVQDVDPWTGEIRQPLAR
                     DPWADEADEAGAAAGADDADEVEDTDETDELDLAAAGEPAPVS"
     gene            complement(1342..>2011)
                     /locus_tag="VLC50_04705"
     CDS             complement(1342..>2011)
                     /locus_tag="VLC50_04705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HSF26800.1"
                     /translation="STGWPLTRWVHRVRRDPLRRLGLPSAERARPSRTALPAPTPAAR
                     AQVDVALREVAEVAAAELPAPWRRSTRQVALAHRDELVTSIGTVVARTDLGVSRRPWW
                     VRLLSALQWALALATVIGVVWLVAALVTDWLGLPELPTYPSGGLPLPTLLVLVGAAGG
                     LALALVGSALARSAGRRAARRVARRLRDAISRVAGQLVVEPVAAEASTHSRLADAVAR
                     VGGR"
BASE COUNT          256 a          772 c          732 g          251 t
ORIGIN      
        1 cagcagccgg cacagcgcga cccggcggcg ctcgccgccg gagagcacgg tgacgtcggc
       61 gtcggggggc gggcagcgca gcgcgtccat ggcctgctcg agctgcgagt cgaggtccca
      121 ggcgccggcg tggtcgatct cctcctgcag ccggcccatc tcctccatga gctcgtcgct
      181 gtagtcggtg gccatcagct cggcgatctc gttgaaccga tcgagcttgg ccttgatgac
      241 gcgcacgccg tcctgcacgt tgcccaagac gtccttggac tcgtcgagct ggggctcctg
      301 ctggaggatg ccgacggagt agcccgggga gaggaacgcg tcgccgttgg acggctggtc
      361 gagcccggcc atgatcttga gcacagtcga cttgccggcg ccgttggggc cgacgacgcc
      421 gatcttggcg ccgggcagga acgacagcgt cacgtcgtcg aggatgacct tgtcgccgtg
      481 cgccttgcgc gccttgcgca tcgtgtagat gaactccgcc atgcgctcca gcctatgggg
      541 cgcacggcgg tgctcacacg gccggctccc gggccccgcg caccggctca ggagacgggc
      601 gccggctcac ccgcggccgc caggtcgagc tcgtcggtct cgtcggtgtc ttcgacctcg
      661 tcggcgtcat cggcgccggc cgccgccccg gcctcgtccg cctcgtcggc ccacgggtcg
      721 cgcgccagcg gttggcggat ctcgccggtc cacggatcga catcctggac gggctggtcc
      781 gccagctcgg ccagagccac ctcgctggcc tgcgcctggg cgtcaccgcc ctggccgcgc
      841 cgcaccttct cgaacgtcgt ctggccccgc gtgaggtcgt ggcccagcgc caccgcgtcg
      901 atctcgaccg acaagcccgc ccgcccgtcc ttgccggtcc agggccggac ccgctgccgg
      961 ccggtgacga gcacaggctg gcccaccttg atgctggcat aggcgttctc ggcgagcgcg
     1021 cgccagcacg tgaccgagta gaagctgctg ggcccgtcct gccacgagtt gcgctcccgg
     1081 tcgaagcgcc gcggcgtgtt ggccacccgg aacgtcgcca tcggcgtccc cccgtcggtg
     1141 acgcgcaggg ccacgccggt caccgcattg ccgacgacgg tcacataggt ctcgttcatt
     1201 ggtcctcccg cacggtcggg tgccgccagt cgacggcaca ggcccgactg tgcggcctcg
     1261 ccgtgcgctc cggcgcggcc gccggcccgg ttgtggacaa cggcggccgg tgcccgacct
     1321 gtggacgacc aggcggagcg gctagcgccc accgacccgg gccaccgcgt cggccagccg
     1381 cgagtgcgtc gaggcctcgg cggccaccgg ctccaccacg agctgaccgg cgacccggct
     1441 gatggcatcg cgcagccgcc gggccacccg ccgcgcggcc cggcgaccgg ccgagcgggc
     1501 cagcgccgag cccaccagcg ccagcgccag cccaccggcg gcgccgacga gcaccagcag
     1561 cgtcgggagc ggcagcccgc cggacgggta ggtcggcagc tcgggcagcc ccagccagtc
     1621 ggtcaccagg gcggcgacga gccagaccac gccgatgacg gtggccaggg cgagcgccca
     1681 ctgcagcgcg gagagcagcc gcacccacca cggccgacgg ctgacgccga gatcggtgcg
     1741 ggccaccacg gtgccgatcg aggtcaccag ctcgtcccgg tgggccagcg ccacctgccg
     1801 agtcgagcgt cgccagggcg cgggcagctc cgcggcggcc acctcggcca cctcacgcag
     1861 ggccacgtcg acctgggccc gcgccgccgg ggtgggagcc ggcagtgcgg tgcgcgaggg
     1921 gcgcgctcgt tcggcggagg gcaggcccag gcggcgcaac gggtcgcgcc gcacgcggtg
     1981 cacccaccgg gtcagcggcc agccggtcga g
//
LOCUS       DATIBB010000125         9186 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_860825, whole genome shotgun sequence.
ACCESSION   DATIBB010000125 DATIBB010000000
VERSION     DATIBB010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 9186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9186
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_860825"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2319
                     /locus_tag="VLC50_04710"
     CDS             <1..2319
                     /locus_tag="VLC50_04710"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HSF26801.1"
                     /translation="EFDYADPMAAGLVRQLRGRAPASPSEVAITEKLAERSGLSIDDT
                     LRLPDADVSLRIVGIVREPDEWNARTAYTLPGAVIDALRASTNEGEAGAYSSASFLVD
                     ASTPVSWPQVMAFNAEGFVVWSRTVMLDPPPANEVPLYQAFGDRQPRGDSAVIVGVVV
                     AAGMALLEVVLLAGPAFAVGARRARRQLAILAAVGAARAHLRDVVLAQGVVLGLVGGL
                     VGTIGGVGLSIAGMPLVEAMDSRDYPPPDLRPLELALLVGVGLVTAVLAAAVPAWQAS
                     RQEVVQALAGRRGQRRTSRRVPLLGLALAGGGTTLAAYGAVERSANAILGGSMIAEVG
                     LVLCTPTLVGWFGRFAPQLPLAPRIALRDAARNRMRSAPAVAAVMAAVAGSVAIGGYV
                     TSLSHHNEAIYVPQAVVGQTVLRSLDGDADLAPALEVARQDLSVTDVLEVHTLGAVCP
                     GDGGCTMIGAATPEANLCPGDPRNSAAPIANATPAGPVDRTDWRCRPAPFERFGLTSY
                     VVASEDLATLTGAHDARAEQVLRAGGVVVTDPRLIHNGQATLAVDEISAAGQQEHRIV
                     EVPAELMPEARMFARAILAPELAQRLDGVTRLAHVVLTTAEPASPAQEQALRDHLAGL
                     SLGSFAVDVERGYTDDYAVGLLALLGAAALITIGTAGIATGLAITDARPDLQTLAAVG
                     AAPGARRRLGMAQSGVITVLGTLLGTAAGLVPAAAVVYALGHVGGVPDDTWSFALPWM
                     HVGVTAVVVPLIAVLAAGLFVRSRIAIERRPG"
     gene            complement(2401..4332)
                     /locus_tag="VLC50_04715"
     CDS             complement(2401..4332)
                     /locus_tag="VLC50_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018888808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HSF26802.1"
                     /translation="MAFYPDSYPPIADHGLIGDLQTAALIATDGTIDWFCAPRFDSPS
                     IFGSLLDLSRGGYVRVAPADGAHTTKQLYFPDTAILITRFLTDGGVGEVLDFMPAQTS
                     QVATDHHRIVRMVRCVRGRMSFDIEAAPRFDYGRKQHSLRLDPNGAVFSAPGEHPLVV
                     HAVREPGDGRLAELRPASTPDDLHLSLTLEAGQMRGAVLEIGGAESPREIRVAEMQTM
                     FEDTVRFWRSWLAQSTYVGRWREALRRSAITLKLMTYAPSGALVAAPTAGLPEQVGGE
                     RNWDYRYTWVRDSSFSVYALLGMGFTDEVAAFGKWLGDRIREHAGGPGGPLHIMYRVD
                     GSSDLSEEELDHWEGYRGSRPVRIGNGAATQRQLDIYGEALDSIFYADAHGVQGSHAA
                     WTTIRELLDWLCEHWDEPEEGIWETRGGQKDFTYGRLMTWVAFDRMIRLARDRARPAP
                     LQRWITERDAVYEQIMSRGWSDKRQAFVQHYETDVLDASLLRMPPVGFVAPRDPMWLS
                     TLDAMDHELVTDSLVYRYDPSASPDGLRGSEGTFSLCTFDYVRALAMAGRLEEARLTF
                     EKMLTYSNHLGLFSEEIGLNGEQLGNFPQAFTHLAQIDAAIRLNEALDARGAATVSFA
                     GVAFPAQRPSAPTEVPATA"
     gene            complement(4739..4999)
                     /locus_tag="VLC50_04720"
     CDS             complement(4739..4999)
                     /locus_tag="VLC50_04720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF26803.1"
                     /translation="MRYGPVVAVVLACAAASGMVYAAVTTYRAGLAPVNSVEPRVSPE
                     PATLAPAPTTDGERAAVVPHPRRSPEASPTAVPSPVGSGSQQ"
     gene            complement(5184..7226)
                     /locus_tag="VLC50_04725"
     CDS             complement(5184..7226)
                     /locus_tag="VLC50_04725"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HSF26804.1"
                     /translation="MLQDVDSLASWLEAVPFGIAFLGTDLRYLLVNRAVCQMTGLPQE
                     RFVGIRIHDVFPNLNPGLDTALRRVWDGHATTGLRVSVAPPTEPGLARYLRVSVFPVF
                     DGAGNIVAASVCSTEVTEQVLAEALRDDAVRVAQGAASSATRLYAVGRALSAARSVDD
                     IAAAVAAECAQPLSARATGLVIVGEDGGSARFMAVEGQLPVSRRWATFRLEEPLIAAE
                     VIRTGVARFDQDPRTWPERFRVAARESMLAGVAAQATVPMVVSGRVLGAMVLAWSTPQ
                     AFPERDQRFLETVAAQSAQALERVRLFEQQQSAAESLQRSLLPEELPTLKGIDTAAAY
                     LPATEGLQVGGDWYDVMELPDGAVLLSLGDVAGSGVPAAVVMGQLRHGLRAIALREPS
                     PARLLDWLEEILDQDDEELATLMLARLDPRTGEVTISNAGHLPPILLPSQGLARLLEI
                     PSSPPLGVGTSLAGHRTDTQLQLAEGHSLVLYTDGLVEVRQLSISLGIDQLREAVDAV
                     PRGPQGQLRAADLCEHVMVSVAAARERSDDVAVLVVHREQSAPALTPRQVMVVTLGLA
                     GEDRDAARAREWVADRLAEWGLTRMADPLRLVVSEMVTNALIHAGGAGELRLRRLHDG
                     VRVEVEDRSANLPRVGELSTEATGGRGLLVIESLADRWGSHAVPGGKIVWAHLAED"
     gene            complement(7485..7937)
                     /locus_tag="VLC50_04730"
     CDS             complement(7485..7937)
                     /locus_tag="VLC50_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013677305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufE family protein"
                     /protein_id="HSF26805.1"
                     /translation="MSVETLPAPLEELVEEFAAVPERDRLQLLLELSYELEPLPAAYA
                     EHRDRLEPVPECQSPLFLAVELTPDQRVRLVFDAPAEAPTTRGFAAILHQGLSGLPAH
                     EVLAVPDAFYLRLGLAGVVSPLRTRGLAAMLARVKRQIRVLLAAASKG"
     gene            complement(7934..8815)
                     /locus_tag="VLC50_04735"
     CDS             complement(7934..8815)
                     /locus_tag="VLC50_04735"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HSF26806.1"
                     /translation="MAVPFDPDPRFADYARPQRLVSTAWLAEHLGHPGLVVAESDEDV
                     LLYDTGHVPGAVKLDWHTDLNDPLTRDYVDGPRFAELMSAKGIGWDTTVVLYGDKNNW
                     WAAYALWVMSLFGHPDVRLLDGGRAAWLTEGRPTTTVVPTPIPADYPVVERVDAPIRA
                     FKDEVLAHLGKPMVDVRSPGEFSGELLHMPDYPQEGAMRGGHIPGAANVPWGRAAAED
                     GRFRSVDELRAIYEGEAGLSAADDVVVYCRIGERSSHTWFVLTHLLGYPSVRNYDGSW
                     TEWGNAVRVPIARGQRP"
     gene            complement(8898..>9186)
                     /locus_tag="VLC50_04740"
     CDS             complement(8898..>9186)
                     /locus_tag="VLC50_04740"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HSF26807.1"
                     /translation="LTWSGTFVHAAPWSSSSQGRANVSHGCINLSVARGKAFFEMSRY
                     GDIVTVVNTARPAKDLVASGDPGMADWNLDWPDYVAGSALDRVVLVTPLTV"
BASE COUNT         1362 a         3270 c         3274 g         1280 t
ORIGIN      
        1 gagttcgact acgccgaccc gatggcggcc gggctggtca ggcagctccg cggccgagca
       61 ccggcctcac cctctgaggt agcgattacc gagaaactgg cggagcgcag cggcctcagc
      121 atcgacgaca ccttgcgcct gcccgacgcc gacgtgtcgc tacgcatcgt tggcatcgtc
      181 cgcgaaccgg atgagtggaa cgctcgcacc gcctacacgt tgcccggtgc ggtgattgac
      241 gctctccgcg cgtcgacgaa cgagggcgag gcgggcgcat actcgtcggc gagcttcctc
      301 gtcgacgcct cgactccggt cagctggccg caggtgatgg cattcaacgc cgaaggcttc
      361 gtcgtgtggt cgcgcactgt gatgctggac ccgccacccg ccaatgaggt ccccctctac
      421 caggcattcg gggaccgtca gccgcgcggc gactccgccg tgatcgtggg ggtcgtcgtg
      481 gcggcgggca tggccctgct cgaagtcgtc ctgctggccg ggccagcttt cgccgtcgga
      541 gcacgaagag cccgccgcca gctcgcgatc ctcgccgcgg tcggagcggc ccgcgcccac
      601 ctgcgcgacg tcgtgctcgc ccagggcgtc gttcttggcc tcgtcggagg cctcgtgggc
      661 acgatcggcg gggtcggtct cagcatcgcc ggcatgccgc tggtcgaggc gatggacagt
      721 cgcgactacc caccgcccga cctgcgcccc ctcgagctcg ccctgctcgt cggtgtcggc
      781 ctggttaccg cggtcctcgc cgcggccgta cccgcgtggc aagcctcccg ccaggaagtg
      841 gtccaagcct tggccgggcg acgcggccag cgacgcacgt caaggcgggt cccgttgctg
      901 gggctggcgc tcgccggtgg cggcacgacg ctggcggcgt acggtgctgt cgagcgctcg
      961 gccaacgcga tcctgggcgg ctcgatgatc gccgaggtcg gactggtgct gtgcaccccg
     1021 acactggtcg gctggttcgg ccgattcgcg ccgcagctgc cactggcacc gcggatcgcg
     1081 ctgcgggacg cggcccgcaa ccggatgcgt tcggcgccgg ccgtcgcggc cgtgatggca
     1141 gcggtcgccg gcagcgtcgc gatcggcggc tacgtgacca gcctgtccca ccacaacgag
     1201 gccatctatg tgcctcaggc cgtggtgggg cagaccgtgc tgaggtccct cgacggcgac
     1261 gccgacctcg caccggccct cgaggtcgcc cgtcaggacc tctcggtgac agatgtcctt
     1321 gaagtgcaca cgctcggggc cgtgtgcccg ggcgatggcg gctgcacgat gatcggcgcg
     1381 gccacgcccg aggccaacct gtgcccgggc gatcctcgaa attctgccgc acccattgcg
     1441 aacgccactc cggccggccc tgtggaccga accgattggc ggtgccggcc cgcgccattc
     1501 gaacggttcg ggctcacgtc ctatgtcgtg gcttcggaag acctcgcaac gctgaccggc
     1561 gcccacgatg cacgggccga gcaggtgctg cgggcgggcg gggtcgtcgt gaccgacccg
     1621 cggctgatcc acaacggtca ggcgacgttg gcggttgacg agataagcgc agccggccag
     1681 caggagcatc ggatagtgga ggttccggct gaattgatgc ccgaggccag aatgttcgcc
     1741 cgagccatcc tcgccccaga gctggcacag cgtctggacg gagtcaccag gctggcccac
     1801 gtggtgctca ccactgccga gccggcgagt cccgcgcagg agcaagcgct gcgcgatcat
     1861 ctcgccgggc tcagtctggg ctcgttcgct gtcgacgtgg agcgcggcta caccgacgac
     1921 tacgcagtcg gcctgctcgc cctgctcggc gccgccgcac tgatcaccat cggcacagcg
     1981 ggcatcgcga ccgggctcgc gatcaccgac gcccgccccg acctacagac cctggcagcg
     2041 gtcggtgccg cgccaggggc gcgacgccgg ctgggcatgg cgcagtcagg agtcatcacc
     2101 gtcctgggca ccctgctggg aacggcggcc gggctggtgc ctgcggccgc ggtggtctat
     2161 gcactcggac acgtcggcgg cgtgccggac gacacgtgga gcttcgcgtt gccctggatg
     2221 cacgtgggcg tcaccgcggt cgtcgtaccg ctgattgctg tgctggccgc cgggttgttc
     2281 gttcgcagcc gcatcgccat cgagcgccgc cccggctgac cacgcacact gcactgatca
     2341 tgcgcgtatg gcggggggcg ccaaacgcgc atgatcagtg caggagaggc gagaatcggg
     2401 ctaggccgtg gcggggacct cggtcggggc ggacgggcgc tgcgcgggga acgccacccc
     2461 ggcaaaggac accgtggcgg caccgcgtgc gtcgagcgct tcgttgagcc ggattgccgc
     2521 gtcgatctgc gccaggtgcg tgaatgcctg cgggaagttg cctagctgct cgccgttgag
     2581 cccgatctcc tcggagaaca gccccaggtg gttggaatag gtgagcatct tctcgaaggt
     2641 cagccgcgcc tcctccaacc ggccggccat cgccagcgcc cgcacgtagt cgaacgtgca
     2701 cagcgagaac gtgccctcgg atccacgcaa cccgtcgggc gaggcgctgg ggtcatagcg
     2761 gtagaccagg ctgtcggtga caagctcgtg gtccattgcg tccagcgtcg agagccacat
     2821 cgggtcgcgc ggcgcgacga accccaccgg cggcatccgc aacaacgacg catcgagtac
     2881 gtcggtctcg tagtgctgca cgaatgcctg gcgcttgtcg ctccagccgc ggctcatgat
     2941 ctgctcgtag acggcgtcac gctcggtgat ccaccgctgc agcggtgcgg ggcgggctcg
     3001 gtcgcgcgcc agccggatca tccggtcgaa cgccacccag gtcatgagac gcccgtaagt
     3061 gaagtccttc tggccgccac gggtctccca gatgccctcc tccggctcgt cccagtgctc
     3121 gcacagccag tcaagcagct cgcggatcgt ggtccaggcc gcgtggctgc cctgcacgcc
     3181 gtgtgcgtcg gcgtagaaga tgctgtcgag cgcctcgccg tagatgtcga gctgacgctg
     3241 ggtcgcggcg ccgttgccga tgcgcaccgg ccgggagccg cggtagccct cccagtggtc
     3301 aagctcctcc tcggacaggt cggacgaccc gtcgacccga tacatgatgt gcagcggccc
     3361 gcccggcccg ccggcgtgct cgcggatccg gtcgcccagc cacttgccga acgcggccac
     3421 ctcatcggtg aatcccatgc ccaacaaggc atagaccgag aatgacgagt cgcggaccca
     3481 ggtgtagcga tagtcccagt tgcgttcccc accgacctgc tcgggcagcc cggccgtggg
     3541 cgccgcgacc agcgcaccgc tcggcgcata ggtcatcagc ttgagcgtga tcgccgaacg
     3601 acgcagcgcc tcccgccaac gtccgacgta cgtcgactgc gccagccacg accgccagaa
     3661 ccgcacggtg tcctcaaaca tcgtctgcat ctcggcgacc cggatctcgc gcggcgactc
     3721 ggcgccaccg atctcgagca ccgccccgcg catctgcccg gcctccagcg tcagcgacag
     3781 atggagatca tcgggggtgc tcgccggccg caactcggcc aaccgcccgt caccgggctc
     3841 ccggacggcg tgtacgacga gcgggtgctc ccccggcgcc gagaacaccg cgccgttggg
     3901 gtcgagccgc agactgtgct gcttgcggcc gtagtcgaac cgaggcgcgg cctcgatgtc
     3961 aaaggacatc cggccgcgca cgcacctgac catgcgcacg atccggtggt ggtcggtggc
     4021 cacctgagag gtctgggccg gcatgaagtc gagcacctca ccgactccgc cgtcggtcaa
     4081 gaatcgggtg atcaggatgg cggtgtcggg gaagtagagc tgcttggtcg tgtgcgcgcc
     4141 gtccgccggg gcgacccgca cataaccgcc ccgcgacagg tcgagcagcg agccgaagat
     4201 gctcggtgag tcgaagcgtg gagcgcagaa ccagtcgatc gtcccgtcgg tggcgatgag
     4261 cgcggcggtc tgcaggtcgc caatcaggcc gtggtcggcg atcggcggat agctgtcggg
     4321 gtagaacgcc atggtcggct cccttttcga cggtcgatgc aacggacggg cagcagaccc
     4381 acgctcgccc gcccagcccg ccacgcgcgt cacccgccga ggatgagttg cgacgtacag
     4441 gcttcgccgg cgcgtgctga caatccggtc atgagggagt ttccatggcg tgacctggcc
     4501 gagaaccggc cggggcaggc ggcgcgcgag caggccgtgg ccgatcgcta aggcctccaa
     4561 cgctgagtgg tcgcttcagc gccgggtgaa gacgggacct atgtcactgt cggcgggcgc
     4621 ggcacaggcg tgcaatcgag gtggcgggaa ggccccggat ccacgacacg gagtaacggg
     4681 agagattccg tgcaccgtgc aatcggaatc cgcgagatgc ggagtgggcg ccttcccgtc
     4741 actgttgcga gccggacccg accggggagg ggacggcggt cgggctcgcc tcgggtgagc
     4801 gccgaggatg cggtacgacg gcggcccgct cgccgtcggt cgttggcgcc ggggcgaggg
     4861 ttgccggctc cggcgagacc cgaggctcga ccgagttgac cggcgccaac ccggcccgat
     4921 aggtggtgac cgcggcatag accatgccgc tggctgccgc acacgcgaga accaccgcaa
     4981 cgaccggccc gtagcgcatg gctggtcccc ctcgtgaagc cggtccgtcc gcccgccccg
     5041 cgcacccctc cgcccgacag ggcacgcgcc cgcatcagtt ccacggtgct cgcgcaccgg
     5101 cgggcatgca acggctgaaa ggtcacggtc cggcagcggg tcacgaatcc ttgccaggca
     5161 acggtttgcc cgcgagcgag tcgtcagtct tcggcgaggt gggcccagac gatcttgcct
     5221 ccgggcacgg catggctgcc ccaccggtcg gcgagggact cgatgaccag cagcccgcgg
     5281 cccccggtgg cctcggtgga cagctcaccg acgcgaggga ggttggccga ccggtcctcg
     5341 acctcgaccc gcacaccgtc gtggagccgg cgcagccgca gctcaccggc accgcccgcg
     5401 tggatgagcg cgttggtcac catctcgctg acgaccagtc gcagcgggtc ggccatccga
     5461 gtcagacccc attcggcgag ccggtcggcg acccactcgc gggcccgggc ggcgtcgcgg
     5521 tcctcgcccg cgagccccaa ggtgaccacc atgacctggc gcggggtcag ggccggcgcc
     5581 gactgctcac ggtgcacgac gaggacggcc acgtcgtcgc tgcgttcacg cgccgccgcc
     5641 acggacacca tcacgtgctc gcacaggtcg gcggcccgca gctggccctg ggggccccga
     5701 ggtacggcgt cgacggcctc gcgcagctgg tcgatgccca ggctgatcga cagctgccgc
     5761 acctcgacca gcccgtcggt gtagagcacg agggagtggc cctcggccag ctgcagctgc
     5821 gtgtcggtgc ggtggccggc cagggacgtg ccaaccccga gcggcggcga agaggggatc
     5881 tcgagcagcc gggccagccc ctgcgacggc agcaggatgg gcgggagatg gcccgcgttg
     5941 gagatcgtca cctcgccggt gcgcgggtcg agccgggcga gcatcagcgt ggccagctct
     6001 tcgtcgtcct ggtcgaggat ctcctcgagc cagtcgagca ggcgggcggg cgagggctca
     6061 cgcagcgcga tggcccgcag cccgtgccgc agctggccca tgaccaccgc cgccggaacc
     6121 ccgcttccgg cgacgtcgcc gaggctgagc agcaccgcgc cgtctggcag ctccatcacg
     6181 tcgtaccagt caccgcccac ctgcagcccc tcggttgccg gcaggtaggc cgcggcggtg
     6241 tcgatcccct tgagcgtcgg gagctcctcc ggcagcaggc tgcgctgcaa cgactcggcg
     6301 gcactctgct gctgctcgaa cagccggacc cgttcgaggg cctgggcgct ttgcgcggcc
     6361 acggtctcga ggaagcgctg gtcacgttcg gggaaggctt gcggggtcga ccaggcgagc
     6421 accatggcgc cgagcacccg cccggagacc accatcggca cggtcgcctg ggcggccact
     6481 ccggcgagca tcgactcgcg agcggccacc cggaacctct cgggccaggt gcgcgggtcc
     6541 tggtcgaagc gcgcgacacc ggtgcggatc acctcggccg cgatcaacgg ctcctcgagg
     6601 cggaaggtcg cccaccgccg cgacacgggc agctgcccct cgaccgccat gaaccgcgcg
     6661 ctacccccgt cctcaccgac gatcaccagc ccggtcgcgc gcgccgacag tggctgggcg
     6721 cactcggcgg ccaccgcggc ggcgatgtcg tcgaccgacc gagccgccga cagggcccgg
     6781 ccgacggcat agagccgggt ggccgaggag gcggcaccct gggcgacccg cacggcgtcg
     6841 tcgcgaagcg cctcggcgag cacctgctcg gtcacctcgg tcgagcacac cgacgccgcc
     6901 acgatgttgc cggccccgtc gaagaccggg aacacgctga cccgcagata gcgggccagc
     6961 ccaggttcgg tgggcggggc caccgagacc cgcaggccgg tcgtcgcgtg gccgtcccac
     7021 acgcgacgca gcgcggtgtc caggccgggg ttgaggttgg ggaagacgtc gtggatccgg
     7081 atgccgacga accgctcctg cggcaggccg gtcatctggc agacggcgcg gttgacgagc
     7141 aggtagcgca ggtcggtgcc gaggaaggcg atcccgaacg ggaccgcctc cagccaggac
     7201 gccaggctgt cgacgtcctg gagcacggct ccggcgtcgg aggagtcacc cgtgccaccg
     7261 gagcgcatgc ccggcacggg cgggtgaggg ggtgacgcgt tcaccggctg ctcaccgcac
     7321 ctccggcgtg tgtgacagtc caattcccca aggcgtcagg ctagacgtcg gcggcgtacg
     7381 acgcaggccc ttgccccctg tttggaccgg tccgcccgat cacagcagcc gggtcattgc
     7441 ctagtcattg cccggtcgag gccgagacat tgccaagccg ttgcctagcc cttggacgcg
     7501 gcggcgagca gcacccggat ctggcgcttg acccgggcca gcatcgccgc cagcccccgg
     7561 gtgcgcagcg ggctgaccac cccggccagg cccagccgca ggtagaaggc gtcgggcacc
     7621 gccagcacct cgtgggccgg caacccgctg agcccctggt gcaggatcgc ggcgaacccg
     7681 cgggtggtgg gcgcctcggc gggggcgtcg aagaccagcc ggacccgctg gtcgggggtc
     7741 agctcgacgg ccaggaacag cggcgactgg cactcgggca ccggttcgag ccggtcgcgg
     7801 tgctcggcat aggcggcagg cagcggctcc agctcgtagg agagctcgag cagcagctgc
     7861 agccggtcgc gctcgggcac cgccgcgaac tcctcgacga gctcctcgag cggggcgggc
     7921 agcgtctcga cgctcacggc cgctgccccc gcgcgatcgg cacccgcacc gcgttgcccc
     7981 attcggtcca ggacccgtcg tagttgcgca cgctcgggta gccgagcagg tgggtgagca
     8041 cgaaccacgt gtgcgaggac cgttcgccga tgcggcagta gacgacgacg tcatcggccg
     8101 cggacaggcc ggcctcgccc tcatagatcg cgcgcagctc gtcgaccgac cggaaccggc
     8161 cgtcctcggc ggcggcccgc ccccagggca cgttggccgc tccggggatg tgccccccgc
     8221 gcatcgcgcc ctcttgcggg tagtcgggca tgtgcagcag ctcgccggag aactcgcccg
     8281 gcgagcgtac gtcgaccatc ggcttgccca ggtgcgcgag gacctcgtcc ttgaacgccc
     8341 ggatcggtgc gtcgacccgc tcgaccaccg ggtagtcggc ggggatcggg gtcggtacga
     8401 cggtcgtggt cgggcggccc tcggtgagcc aggcggcccg accaccgtcg agcagccgca
     8461 cgtcggggtg accgaacagc gacatcaccc acagcgcgta ggcggcccac cagttgttct
     8521 tgtcgccgta gagcaccacc gtggtgtccc agccgatgcc cttggccgac atgagctcgg
     8581 cgaagcgcgg accgtcgacg tagtcgcggg tcagcgggtc gttgagatcg gtgtgccagt
     8641 cgagcttgac cgcgccgggg acgtgcccgg tgtcatagag cagcacgtcc tcgtcggact
     8701 cggcgaccac cagcccgggg tgcccgaggt gctcggccaa ccacgcggtc gacaccagcc
     8761 gttgcgggcg ggcgtagtcg gcgaaccgcg ggtctgggtc gaaggggaca gccacggcag
     8821 gtcctctcgt cgatcgggca ccaccaccgt gacacacggg gccggtcggc gcaccaccgg
     8881 ccgtcgtgtc gcggcggcta gacggtcagc ggggtgacga ggacgacccg gtccagcgcc
     8941 gacccggcga cgtagtcggg ccagtcgagg ttccagtcgg ccatgccggg atcgccggag
     9001 gccaccaggt ccttggcggg ccgcgcggtg ttgacgacgg tgacgatgtc gccgtagcgc
     9061 gacatctcga agaacgcctt gccgcgcgcc acgctcaggt tgatgcagcc gtgcgagacg
     9121 ttggcgcgcc cctggctgct cgaggaccac ggggcggcgt gcacgaacgt gccgctccag
     9181 gtcaat
//
LOCUS       DATIBB010000126         2844 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_1782057, whole genome shotgun sequence.
ACCESSION   DATIBB010000126 DATIBB010000000
VERSION     DATIBB010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2844
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1782057"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..586)
                     /locus_tag="VLC50_04745"
     CDS             complement(<1..586)
                     /locus_tag="VLC50_04745"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009190404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HSF26808.1"
                     /translation="MALQTSPEHPAAVRTIASGIAEWIHRLGAVWVEGQVTQLTRRPG
                     TRTIFLTLRDTDATISLPVTCRREVLDGLEIPLADGARVLVHARPSFYVARGTLSLAA
                     DEIRPVGVGELLARLEHLKRVLAAEGLFEPARKRPLPFLPRLVGLVCGRASAAERDVV
                     DNARRRWPAVRFAIREVAVQGPSAAAEVASAVREL"
     gene            675..1649
                     /locus_tag="VLC50_04750"
     CDS             675..1649
                     /locus_tag="VLC50_04750"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HSF26809.1"
                     /translation="MPATTRKILLAKPRGYCAGVDRAVTTVEKALELYGPPVYVRKQI
                     VHNTFVVRSLESKGAIFVDETEEVPVGATVVFSAHGVAPGVHEEARDRSLKTIDATCP
                     LVTKVHHEARRFAQDDYDILLIGHEGHEEVIGTSGEAPEHIHLVQHADEVDSLVVRDP
                     DKVAWLSQTTLSVDETMAIVDRLRERFPKLIDPPSDDICYATQNRQGAVKEMAEQAQL
                     VIVVGSRNSSNSVRLVEVARECGSAAHLVDGADEIDPAWLDGVDVAGVTSGASVPDIL
                     VRDVLDWLQGHGFGEVEEVVSAQESLIFALPPELRRDLRAAGRLANAR"
     gene            complement(1680..2078)
                     /locus_tag="VLC50_04755"
     CDS             complement(1680..2078)
                     /locus_tag="VLC50_04755"
                     /inference="COORDINATES: protein motif:HMM:NF042451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6542 domain-containing protein"
                     /protein_id="HSF26810.1"
                     /translation="MTAGPVDPPTARPAESGLRLPGLLAVLLVGTAVGATVDLMVSGK
                     YGWGINVAFAISALLAASTVRRRDLLAAVLAAPIAFAVVVTVGLLVTDGYDGLIKTGL
                     LLATELALLAPGVWLGTAIAAAVALLRRRG"
     gene            complement(2128..>2844)
                     /locus_tag="VLC50_04760"
     CDS             complement(2128..>2844)
                     /locus_tag="VLC50_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020580288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HSF26811.1"
                     /translation="EQPTVLDGDQARRPDLVVNLAGGKHLVIDAKVPLGAYLQAVESP
                     DGAARAAGLDAHAKALRAHVDALAAKEYWRLLQPTPEMVILFVPGEAFLAPALEHDPT
                     LLEHAMSRRVIIATPTTLVAMLRTVAYAWAQQALTEHAREVFELGRELYTRLGRLGGH
                     LDKLGRSLHRAVGDYNATVGSLERQVLPQARRFATLRVTDGELTSPTPVEDSPRGLTA
                     GELLEDAAAASALRVVGDDT"
BASE COUNT          417 a         1041 c         1029 g          357 t
ORIGIN      
        1 cgagctcgcg gaccgcgctg gccacctcgg ccgctgcgga cgggccctgc accgcgacct
       61 cgcggatggc gaagcgcacc gccggccaac gtcgccgggc gttgtcgacc acgtcgcgct
      121 cggcggccga ggcccggcca cagaccagcc cgaccaaccg cggcaggaac ggcaacgggc
      181 gcttgcgggc cggctcgaac agcccctcgg cggcgagcac ccgcttgagg tgctcgagcc
      241 gggccagcag ctcgcccacg ccgacggggc ggatctcgtc ggccgccaac gacagcgtgc
      301 cgcgggcgac gtagaagctc ggccgggcgt gcacgagcac ccgggctccg tcggccaacg
      361 ggatctccag cccgtcgagg acctcccggc gacaggtcac cggcagcgag atggtggcat
      421 cggtgtcgcg cagggtgaga aagatcgtgc gggtgccggg gcggcgggtc agctgggtca
      481 cctgaccctc cacccagacc gcacccagcc ggtggatcca ctcggcgatc ccgctggcga
      541 tcgtgcgcac cgccgccggg tgctcgggcg aggtctgcag agccatgccg gtgacgctag
      601 cggtccccac ccacgggcac cgacgtggtg aaggccaccc ggctcggcgc cgctgcgatg
      661 cctacgatgg ccccatgccc gccacgaccc gcaagatcct gctcgcgaag ccgcgtggct
      721 actgcgccgg cgtcgacagg gccgtgacca ccgtcgagaa ggcgctcgag ctctacggcc
      781 cgccggtcta cgtgcgcaag cagatcgtgc acaacacgtt cgtcgtacgc agcctcgaga
      841 gcaagggcgc gatcttcgtc gacgagaccg aggaggtgcc ggtcggggcc accgtggtgt
      901 tctcggcgca cggggtcgcc ccgggcgtgc acgaggaggc gcgcgaccgc tcgctcaaga
      961 ccatcgacgc cacctgcccg cttgtgacca aggtgcacca cgaggcgcgc cggttcgccc
     1021 aggacgacta cgacatcctg ctcatcggcc acgagggcca cgaggaggtg atcggcacct
     1081 ccggcgaggc gcccgagcac atccatctgg tccagcacgc cgacgaggtc gacagcctgg
     1141 tcgtgcgcga ccccgacaag gtcgcctggc tgtcccagac cacgctgtcg gtcgacgaga
     1201 ccatggcgat cgtcgatcgg ctgcgcgagc ggttccccaa gctcatcgac ccgcccagcg
     1261 acgacatctg ctacgcgacc cagaaccgcc aaggtgccgt caaggagatg gccgagcagg
     1321 cccagctggt catcgtggtc ggctcgcgca actcgtcgaa ctcggtgcgg ctggtcgagg
     1381 tggcccgcga gtgcgggtcg gcggcccacc tggtcgacgg cgccgacgag atcgacccgg
     1441 cctggctcga tggcgtcgac gtcgcggggg tcaccagcgg ggcctcggtg cccgacatcc
     1501 tggtgcgcga cgtgctcgac tggctgcagg gccacgggtt cggtgaggtc gaggaggtcg
     1561 tctccgccca ggagagcctg atcttcgcgc tgccccccga gctgcgtcgc gatctgcgtg
     1621 ccgccggtcg gctggccaac gcccgctagc tcggccgatc ctcgatcgac cgcgacgcgc
     1681 tagccgcgtc ggcgcaggag cgcgaccgcc gcggcgatcg cggtgcccaa ccacaccccg
     1741 ggcgcgagca gagccagctc ggtggccagc agcagcccgg tcttgatcag cccgtcgtag
     1801 ccgtcggtga ccagcaggcc cacggtcacc acgaccgcga aggcgatcgg agcggccagc
     1861 accgccgcca gcaggtcgcg gcgtcgcacc gtggaggcgg ccagcagggc cgagatcgca
     1921 aacgcgacat tgatgcccca gccgtacttg cccgagacca tcaggtcgac ggtcgctccg
     1981 accgcggtcc cgaccagcag cacggcgagc agcccgggca gcctcaggcc cgactcggcg
     2041 gggcgtgcgg tgggcgggtc gacgggtccg gcggtcaccc gatcacgcta ccggggccac
     2101 gctgtgtcgc cgttcgcaca cctgccctca cgtgtcgtcg ccgaccacgc gcagcgcgct
     2161 cgccgcggcc gcgtcctcga gcagctcgcc ggcggtcagc ccccgcggcg agtcctcgac
     2221 cggggtggga ctggtcagct cgccgtcggt gacccgcagc gtcgcgaacc ggcgggcctg
     2281 gggcagcact tgacgctcga gcgagccgac ggtggcgttg tagtcgccga cggcccgatg
     2341 caacgagcgg ccgagcttgt cgaggtgccc accgagccgg ccgagccgcg tgtagagctc
     2401 acggcccagc tcgaagacct cgcgggcgtg ctcggtcagc gcctgctgcg cccaggcgta
     2461 ggcgaccgtg cgcagcatcg ccacgagcgt cgtcggggtg gcgatgatca cgcgtcggga
     2521 catcgcgtgc tcgagcaggg tcgggtcatg ctcgagcgcg ggcgcgagga acgcctcgcc
     2581 gggcacgaag aggatcacca tctcgggagt gggctgcagc agccgccagt attccttggc
     2641 ggccagcgcg tcgacgtggg cacgcagggc cttggcgtgg gcgtcgagcc cggccgcccg
     2701 ggccgccccg tcggggctct cgaccgcctg caggtaggcg cccagcggca ccttcgcgtc
     2761 gatgacgagg tgcttgccgc cggcgaggtt gaccaccagg tcgggtcgac gtgcctggtc
     2821 gccgtcgagc acggtcggct gctc
//
LOCUS       DATIBB010000127        23412 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_1912243, whole genome shotgun sequence.
ACCESSION   DATIBB010000127 DATIBB010000000
VERSION     DATIBB010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 23412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23412)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23412
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1912243"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..496
                     /locus_tag="VLC50_04765"
     CDS             <1..496
                     /locus_tag="VLC50_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011606815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HSF26812.1"
                     /translation="EGLVMATRSGSVDPGMLLWLLRHGGHALDEVEEALERRSGLAGL
                     SGTSGDLRDVLARRDAGDAAATLAVEVYVHRLAREVAAMTAATGGLDALVLTGGVGEN
                     APDVRAALAARLGYLGVAVDAGLNASARPDVDVAPADAPVRMLVVAAREDLEVARQTR
                     AVLR"
     gene            514..1515
                     /locus_tag="VLC50_04770"
     CDS             514..1515
                     /locus_tag="VLC50_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019069602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HSF26813.1"
                     /translation="MPTGPAAALPEGLADPELAAWVAEVRREPGEPVGSIGSARLREA
                     ARRRAADRPRGPELAVVKDLCTDNGIALRLYRPTLRPAPMVVFLHGGGFVIGDLDTHD
                     GICRHLAADARAAVVAVDHRRAPEHPGPAAVDDAVDAVSWTRAHLVNLGGLSDAGVAL
                     AGDSSGGAVAVLAASRLRETETSPSALLLAYPNVDMTLSMPSVEGKGHGWGLDSEGLR
                     WFVDQWLGESGRGDDPRLSPWHADLSGLPPTIIATAEHDPLRDEGDALAARLGELGVP
                     VHHLPHAGLVHGFLGLGHVSPTAALAGHALFARFGDLLRELSGPARGQAATKTASSV"
     gene            complement(1482..2336)
                     /locus_tag="VLC50_04775"
     CDS             complement(1482..2336)
                     /locus_tag="VLC50_04775"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005462337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HSF26814.1"
                     /translation="MSSSKSEVTVAPTQPHDRAGRNLVAATGVGLVLGGLVLGSLFVR
                     KELFVGLVAIAVVLAIWELATALASGGPRVALVPIAFGAAVMVVAAFVAGPDGLLVAA
                     TVTVLVVLVWRLPAGVAGYVRDVTASTFIVLYVPLLAGFTMLMLRADDGPVRLVVFFL
                     VTVCSDVGGYAAGVTLGRHPMAPSVSPRKSWEGFAGSVTACAVGGALSVVLLLDGLWW
                     QGVLVGLAAVCTATLGDLAESMVKRDLGLKDMGSLLPGHGGLMDRLDSLLPTAPVVYT
                     LLAVFVAA"
     gene            complement(2317..2874)
                     /gene="frr"
                     /locus_tag="VLC50_04780"
     CDS             complement(2317..2874)
                     /gene="frr"
                     /locus_tag="VLC50_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020138771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HSF26815.1"
                     /translation="MIDETLFEAEEKMDKALSVAREDFAAIRTGRVTPAMFNKILVDY
                     YGAPTPVNQLASFHQQEGRNILVAPYDKGSLAAVEKAIRDSDLGVNPTNDGERIRVVF
                     PELTEERRKDYVKVARHKAEEARVSVRSVRRRAKETLDRLSRDGEVGEDEAGRAEKEL
                     EKLTARFVASIDDLLKNKEDELLEV"
     gene            complement(2892..3629)
                     /gene="pyrH"
                     /locus_tag="VLC50_04785"
     CDS             complement(2892..3629)
                     /gene="pyrH"
                     /locus_tag="VLC50_04785"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020553147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HSF26816.1"
                     /translation="MLKLSGEVFGGGRFGIDPDVVQSIARQIADVVRAGSQVAIVVGG
                     GNFFRGAELQQRGMDRARSDYMGMLGTVMNCLALQDFLEKQGVETRVQTAITMGQVAE
                     PYIPRRAVRHLEKGRVVIFGAGAGMPYFSTDTVAAQRALEIGCGALLMGKNGVDGVYD
                     ADPRTDPQARRFETVGYAEVIRRGLKVADAAAFSLCMDNRLPIIVFNLLDEGNIARAV
                     AGEKIGTLVTSQADHTTTPPRRAPATD"
     gene            complement(3820..4641)
                     /gene="tsf"
                     /locus_tag="VLC50_04790"
     CDS             complement(3820..4641)
                     /gene="tsf"
                     /locus_tag="VLC50_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009074497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HSF26817.1"
                     /translation="MANFTAADVKRLRDATGAGMMDAKRALEETDGDFDKAVEELRIK
                     GAAKAAKRGESRTASNGLVASTDGAMIELACETDFVAKNESFQALASEIVALAAAAKI
                     GDVETLLAQALPDGRTVAESITSLAATIGEKLELRRVVVLEGQVATYMHKRASDLPPQ
                     VGVLVAFEGDDLDVARGAAMQIAAMRPQYVAREEVPAEVVANEARIAEATAREEGKPE
                     GALPKIIEGRVNGFYKDVVLLEQASVQDHKKSVKALLDAAGVKVTRFARFEVGAS"
     gene            complement(4747..5649)
                     /gene="rpsB"
                     /locus_tag="VLC50_04795"
     CDS             complement(4747..5649)
                     /gene="rpsB"
                     /locus_tag="VLC50_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020553149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HSF26818.1"
                     /translation="MAVVTMRQLLESGVHFGHQTRRWNPKMKRYIFTERNGIYIIDLQ
                     QSLSYIDRAYDFVKQTVAHGGTILFVGTKKQAQETVAEQATRVGMPYVNERWLGGMLT
                     NFQTVHKRLQRLKELEEIDFDDVAGSGRTKKELLVLKREKDKLEKTLGGIRNMSRVPS
                     AVWVIDTKKEHLAVNEARKLGIPVVAILDTNCDPDEVDYKIPGNDDAIRSVTLLTRVL
                     ADAVADGLMARAGAGKEAPAEGVATDEPLAEWEQELLAGASAEAAPAEAAPAEATPTA
                     EQAPQAQAAPAEASEGEQPEATQA"
     gene            5936..7117
                     /locus_tag="VLC50_04800"
     CDS             5936..7117
                     /locus_tag="VLC50_04800"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015577797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HSF26819.1"
                     /translation="MATGRLVERMQSFGTTIFAEMSALARAHDAVNLGQGFPDTDGPD
                     EVLEAAVAAIRSGHNQYPPGRGIPELLDAVAAHQKRWYGVDLDPTTEVLITAGATEAI
                     AASLTALAGPGDEVLMFEPYYDSYAACVALGGATRRCVTLRAPDFAVDLDELRAAVSP
                     RTRVVLLNSPHNPTGKVFTRGELEAIAAVCIEHDLVAITDEVYEHLVYDGQHVPLSTL
                     PGMRERTVTISSAAKTFSVTGWKVGWVSGPAELVSAVLTVKQFLTYVNAAPLQPAVAA
                     ALALPDAYFDQLRDSHRDRRDRLVAGLRGAGLTTFVPAGTYFVTTDIASIGERDGLAF
                     CRELPARIGVAAVPEAVFYDDTEAGRSLVRFAFCKRPELIDEGVRRLAGLATARAGQP
                     G"
     gene            7214..7876
                     /locus_tag="VLC50_04805"
     CDS             7214..7876
                     /locus_tag="VLC50_04805"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HSF26820.1"
                     /translation="MRTTSRTLLAALLTVALTVLGGGPTWADATAPVPPGPDRWAAPV
                     QPLRLARGFAPPAQRWLPGHRGIDLAASVGAEVRAVDAGVVVWAGDLAGIAVVSIAHP
                     NGLRSTYQPVEASVEIGDLVARGQPLGTVTVAGSHCAPAACLHLGAKRGQTYLDPSLL
                     LGLRRPRLLPYLTLDPTGATGVAPTAPDTPVTPAASPGLVDAVSDVLASMLAALGQAA
                     LT"
     gene            complement(7887..9125)
                     /locus_tag="VLC50_04810"
     CDS             complement(7887..9125)
                     /locus_tag="VLC50_04810"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HSF26821.1"
                     /translation="MMRSTTETVDSSAGSRTNPAWGSDWAQVAALWRLDPAMTHLNHG
                     SFGAVPTPVLEEQHRWRDRMETNPQRFFTRGVTESLVAARGEIAQFFGLGADELALVR
                     NATTGVNTVFACAGLQPGDEVLLTEHGYGAVRIAADRACARAGARVVLAAVPLAVTDD
                     EIVDRVLAAVTPRTRLAVIDQVTSATARAMPLDRLVPALQERGVAVCVDAAHAPGMLD
                     VDLARLAPDYWTGNLHKWAWAPRGTAVLYAAPTRRTGLRPLVASWNDDLGFPGAFDRT
                     GTDDLTAWLAAPAALRLMKELGLDRVREHNRRLVQQGRATLATAVSEQVPEAAVLPTS
                     DGSTLSMALVALPPGVAVDLAGAEAWKRRIAEHAGVEVAVTTFRGQGLLRLSAQVYNC
                     PADYEVLARRLPTLLADAAA"
     gene            complement(9129..9959)
                     /gene="whiG"
                     /locus_tag="VLC50_04815"
     CDS             complement(9129..9959)
                     /gene="whiG"
                     /locus_tag="VLC50_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor WhiG"
                     /protein_id="HSF26822.1"
                     /translation="MKPDEVGVSRPGVDDAVDEVAHNEMVLRQIWRDFKDSGDPTLRE
                     RLILHYSPLVKYVAGRVGVGLPPNIEQADLVSYGIFGLIDAIEKFDLERAIKFETYAI
                     SRIRGAIIDELRTIDWVPRSVRSKARDVEKAYAALEAQLHRTPTEAEVAAEMGIALSD
                     LHHIFSQVSFVNVVALDELLSVSGEKGDKLSLVDTLEDTKAEDPVVAFESEETKYLLA
                     KAINGLPEREKIVVTLYYYEGLTLAEIGQVLGVTESRICQMHTKAVLQLRSRLADNHD
                     "
     gene            complement(10081..10992)
                     /locus_tag="VLC50_04820"
     CDS             complement(10081..10992)
                     /locus_tag="VLC50_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016887779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HSF26823.1"
                     /translation="MPDELAGMLDEFARHLGAERGCSRHTVRAYRSDVASMLEHAQRM
                     GRAQLTEIDVSTLRSWLARSSTGGAARATIARRAAAVRSFTAWARRHGHLVSDPGLLL
                     GTLKTRRALPDVLDRAEATALLDACAEQAHDDDPVALRDLAMVELLYATGIRVGELVG
                     LDVDDLDRTRHVVRVFGKGAKERTVPVGAPALRAVDRWLSRGRPHLCTGRSGPAVFLG
                     ARGGRVDPRVVRSVVHRLVQTVESAPDLGPHGLRHSAATHLLEGGADLRSVQEILGHA
                     TLATTQIYTHVSVERLKATYELAHPRA"
     gene            complement(11098..12279)
                     /gene="dprA"
                     /locus_tag="VLC50_04825"
     CDS             complement(11098..12279)
                     /gene="dprA"
                     /locus_tag="VLC50_04825"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HSF26824.1"
                     /translation="MSVDPAERRARAALSRLTEPGDDRLAALVEELGAVEVLARLRER
                     RLPGSSAAHWSARLATLDLRADVARARAVRARYVVPGDDEWPDQLDDLAGLRDQQMSH
                     AARPLGLWVRGPAHLRELTERAIAVVGSRAATEGGCYITDEMCTTLAERGWTIVSGAA
                     YGIDSHAHEGALAGDGPTVAVLACGVDVAYPRAHAGLLARIARSGLVVSELSPGCSPT
                     RVRFLRRNRVIAALAQATVVVEAAHRSGALTTASFADALGRWVGAVPARVGDQQSHGC
                     LTLLRRPGTVLVRGAADVLEVVGQMGVDAVVEGRGPETPLDGLDELTRRVVEALPVRQ
                     GAEAASISRVAGLELLSVHAALTRLELAGLAEHRSGLWRRCRPRRDEPGSARQVGATL
                     F"
     gene            complement(12292..13842)
                     /locus_tag="VLC50_04830"
     CDS             complement(12292..13842)
                     /locus_tag="VLC50_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011981965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HSF26825.1"
                     /translation="MTLARTRTIALLGLQGRVVDVEVHIGAGLPAVIITGLADTALGE
                     ARDRVRAAVVNSGFDWPQHRITVNLAPGDLPKHGTGYDAAIAAGILLAMRAFGPAPLR
                     GAVLLAEVSLDGRLRPVRGVLPAVMAAAAAGIGRVVVAEASAAEARLVPGVDVVALPS
                     LRALADWVSGEWTPAATAPSSPSTPSVSAPARDLADVVGQRLGRHVLEVAAAGGHHLL
                     LTGPPGAGKTMLAERLPGLLPPLPPEAALETTAVHSVAGLLEPGAPLVDRAPYQDPHH
                     SASRAAIVGGGSGHPRPGALSLAHNGVLFLDEAPEFSADVLEALRQPLESGEVVIARA
                     SATARYPARFQLVLAANPCPCGLSASRGETCRCTPAAKRRYASKLSGPLLDRVDLQVE
                     LPPVSRAELAAERRGEASTVVAERVAAARERMRHRLGRDEGSTGAGWRCNGEIPGYVL
                     RRRWPVPPDVAAPAHALLQAGRLTARGVDRVLRVAWTLADLAGLPAPSADHVYAAAAL
                     RSAHRVAA"
     gene            complement(13846..14202)
                     /locus_tag="VLC50_04835"
     CDS             complement(13846..14202)
                     /locus_tag="VLC50_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015883019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HSF26826.1"
                     /translation="MRAKDVLGQYGERLAEAHLVGQGMVVLDRNWRCELGEIDLVARD
                     GDALVVCEVKTRSGLGFGHPLETVTAVKLARLRRLAVRWVADHGVHPPQIRLDVVGVL
                     RRGSGPAQIEHVRGVG"
     gene            complement(14308..14646)
                     /locus_tag="VLC50_04840"
     CDS             complement(14308..14646)
                     /locus_tag="VLC50_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020380097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2469 domain-containing protein"
                     /protein_id="HSF26827.1"
                     /translation="MSAEDLEKYETEMELQLYREYRDVVGLFSYVVETERRFYLTNNV
                     DLQARTADGEVYFEVTMSDAWVWDMYRPARFVKNVKVVTFKDVNVEELAKSDLELPKD
                     LPGGRDFGRS"
     gene            complement(14643..15398)
                     /locus_tag="VLC50_04845"
     CDS             complement(14643..15398)
                     /locus_tag="VLC50_04845"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013770668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HSF26828.1"
                     /translation="MLRESGELLVAGVDEVGRGALGGPVSVGVVVVDLAVATAPRGLR
                     DSKLLTPNARGALVPRLRRWARAWAVGHASAAEIDELGILCALRLAGERAFADLPVAP
                     RLVLLDGSYDWLTRPTVPPSLFDDAEADETDEAVTRSVTTLVKADLRCASVAAASVLA
                     KTTRDALMVEMAHTYPAYGWESNKGYASVEHLEAMRSFGVCEQHRRSWNVRALAMRED
                     DGMDVGLDVGLDLTADIGLDDLLAEQVPAESRP"
     gene            complement(15470..15976)
                     /locus_tag="VLC50_04850"
     CDS             complement(15470..15976)
                     /locus_tag="VLC50_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018102381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HSF26829.1"
                     /translation="MTSTGLAPIRRDSARVVLLDEQQRVLLFHGADPDRPEARFWFTP
                     GGGVDPGESPTECALRELREETGLRGVELGPLVWRRRARFSFCGQTYDQREVYFLVRA
                     PSFTVDTSGFTELERRATSGHRWWTLEAMAAESIVVAPAELPVRLAELLRNGPPATPV
                     NLSGAGQS"
     gene            complement(15987..16787)
                     /gene="lepB"
                     /locus_tag="VLC50_04855"
     CDS             complement(15987..16787)
                     /gene="lepB"
                     /locus_tag="VLC50_04855"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HSF26830.1"
                     /translation="MNHDGTRGADEVGMDPVTSTGSVPAPRNPRSAGRPAARHVLPRR
                     PARRPMVVEIAIVVVLSLALSALIKTFLVQAFYIPSGSMENTLQLGDRILVNKLATRF
                     DDLHRGDVVVFEDPGGWLHPEDLPQREGLAKLAHDVFVGLGVLPSEQAGDLVKRIIGL
                     PGDHVVCCDDQGRVTVNGVALSEPYLYPGNEPSDTPFDVKVPAGQMWVMGDHRQVSQD
                     SRAHQEQTLHGMVPMADVVGRVFVVIWPFDRFQRVERPASFGQVAAGP"
     gene            complement(16784..17524)
                     /gene="lepB"
                     /locus_tag="VLC50_04860"
     CDS             complement(16784..17524)
                     /gene="lepB"
                     /locus_tag="VLC50_04860"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HSF26831.1"
                     /translation="MRLRAPRGPGGSRWPVWVEVAVLVLLALVVAILVKAFLVQAYVI
                     PSGSMQPTLRVGDRVLVDKVSLHVRDPRRGEVVVFDGVDSFVPEAAARRASGLRALAD
                     DLLDLVGLGPPGEHDFIKRVIGVAGDRVRCCDPDGRVTVNGTALDETSYLFPGNRPST
                     MSFDVVVPPGRLWVMGDHRAQSQDSRAHLGDPGGGMVPVDRVLGRAVAVVWPPPHWAG
                     LGTPPAFALGAIFVPFVRGGDLRRTVRW"
     gene            complement(17553..17915)
                     /gene="rplS"
                     /locus_tag="VLC50_04865"
     CDS             complement(17553..17915)
                     /gene="rplS"
                     /locus_tag="VLC50_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HSF26832.1"
                     /translation="MHTLDSVDAASLRDDLPDFRPGDTLKVHVRVVEGNRSRVQVFQG
                     VAIRRQGSGVRETFTVRKVSFGVGVERTFPLHTPIIDRIEVVTRGDVRRAKLYYLRDL
                     RGKAAKIKEKRDTSAAGR"
     gene            complement(18055..18867)
                     /gene="trmD"
                     /locus_tag="VLC50_04870"
     CDS             complement(18055..18867)
                     /gene="trmD"
                     /locus_tag="VLC50_04870"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019876433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HSF26833.1"
                     /translation="MRIDVLTIFPDYLAPLGLSLIGKARAEGLLDVRVRDLRDWTTDR
                     HRTVDDTPYGGGAGMVMKPEPWGAALEEVAGDGADAPVVLVPTPSGRPFTQALAHELA
                     QASRLVFVCGRYEGIDARVVEEAASRWRVEEVSLGDYVLNGGEVAVLVVVEAVARLLP
                     GVIGNAESLVEESHALGSGLLEYPVYTKPASWRGREVPPVLLSGNHAAVDRWRRDESL
                     RRTATRRPDLLAALPLDGLDAADLQLLAGLGWAPGADGRLTRFAPAAPAVAD"
     gene            complement(18870..19385)
                     /gene="rimM"
                     /locus_tag="VLC50_04875"
     CDS             complement(18870..19385)
                     /gene="rimM"
                     /locus_tag="VLC50_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017576213.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HSF26834.1"
                     /translation="MQVVVGRIGRAHGVRGEVLVDSRTDSPDQRFVAGAVLATEPASS
                     GPLTVTHAREHSGRLVVAFAQAGDRTAAEALRGTLLLAEVDPHEAAGPDEFYDHQLVG
                     LRARTVAGEDVGEVVAVQHGGAQDLLVVRREGADVLVPFVSAIVPAVDLAAGHVLLDP
                     PAGLLDLETAP"
     gene            complement(19402..19641)
                     /locus_tag="VLC50_04880"
     CDS             complement(19402..19641)
                     /locus_tag="VLC50_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012853744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HSF26835.1"
                     /translation="MLEEALEHLVKGIVEHPDDVKVRDRALRRGRVLEVRVHPSDLGK
                     VIGRGGRTAKALRTVIGALGPGRGVRVDFLDVDER"
     gene            complement(19644..20129)
                     /gene="rpsP"
                     /locus_tag="VLC50_04885"
     CDS             complement(19644..20129)
                     /gene="rpsP"
                     /locus_tag="VLC50_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012853745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HSF26836.1"
                     /translation="MAVKIKLLRMGKIRAPYYRIVVADARTKRDGRAIEEIGKYHPTE
                     EPSFIEVKSERVQYWLGVGAQPTEPVKAILKVTGDWQKFRGEPGAEGTLKTAAPRADK
                     KAIFEAAAKETLGEPKAAATTPKKKAAKAEPKPDEAKAEPAAEAPASDEAKAAETQGE
                     S"
     gene            complement(20328..21440)
                     /locus_tag="VLC50_04890"
     CDS             complement(20328..21440)
                     /locus_tag="VLC50_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014377500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HSF26837.1"
                     /translation="MDASSGRAPTKTLHLRGVVLPEGEQRDLWVREGVVTTEPVAGAQ
                     TVVDGGWIMPGLVDAHCHVGLDANGAVPPDVQEEQARADLDVGTLLIRDAGSAADTRW
                     IDERDDLPRIIRAGRHIARSRRYIRNYGVEVDPADLVAAVEEQAKRGDGWVKLVGDWI
                     EREVGDLTPCWPREVLEAGIGRAHELGVRVTAHVFGEDALPDLLGAGIDCIEHGTGLT
                     DATIAMMAERGTALVPTRIQIDNFPHYAAAGEDKFPRYSAHMRHLHAGVDATLRAAHE
                     AGVPIYCGTDAGGVLPHGLAAEEITAMCGFGMSPAEALAAGSWAARAWLGLPARLDEG
                     TPADLVVYDEDPRLDLTTLRRPRTIVLRGRVVAPTR"
     gene            complement(21452..23011)
                     /gene="ffh"
                     /locus_tag="VLC50_04895"
     CDS             complement(21452..23011)
                     /gene="ffh"
                     /locus_tag="VLC50_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020577684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HSF26838.1"
                     /translation="MFETLSDRLAVTFKGLRGKGRLTEADVDATCREIRVALLEADVA
                     LPVVRSFIGKVKTRALGADVSQALNPAQQVVKIVDEELVEILGGQTRRVTFAKTPPTV
                     IMLAGLQGAGKTTLAGKLALWLKGQGHQPLLVAADLQRPNAVTQLQVVGDRAGVTVFA
                     PEPGNGVGDPVAVARASIEHARTKLFDVVVVDTAGRLGVDAELMAQAADIRAAVSPDE
                     VLFVLDAMIGQDAVTTAQAFLDGVDFTGVVLTKLDGDARGGAALSVRELTGRPIMFAS
                     NGEKLEDFDVFHPDRMASRILGMGDMLTLIEQAEKTFDQEQAAKMASKLQRGDGFTLE
                     DFLAQMEQVRKMGSLSKILGMLPGMGQVREQLESIDEREIDRVAAIIKSMTPQERADP
                     RILNGSRRLRIARGSGCQTSDVNNLVERFGQAQKMMRQMGKGGLRGMPGLPGMPAMPG
                     MAGPGGRSKKAKGKAAGKRGKGARSGNPAKRAAAERAIEGASGDASMPALPADFELPA
                     ALREMLPPDGH"
     gene            complement(23062..>23412)
                     /locus_tag="VLC50_04900"
     CDS             complement(23062..>23412)
                     /locus_tag="VLC50_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019931794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF26839.1"
                     /translation="DSLDVVGVHLVGGMVGTLLVGLLASGAITGGPAGLLYGGQWSQL
                     ARQAVAALVVAGYSFALAWVIGSVIDRTIGLRVDEADERVGIDLAVHAESAYALAPVP
                     GTALAQRGPVARLP"
BASE COUNT         3141 a         8614 c         8495 g         3162 t
ORIGIN      
        1 ggaggggctg gtgatggcca cccggagcgg ctcggtcgat cccgggatgc tgctgtggct
       61 gctgcgccac ggcgggcacg ccctcgacga ggtcgaggag gcgctggagc gccggtcggg
      121 gctggccggg ctgtccggca cctcgggcga cctgcgcgac gtgctggcgc gccgcgacgc
      181 cggcgacgcg gcggcgacgc tggccgtcga ggtctacgtg caccggctgg cccgcgaggt
      241 ggccgcgatg accgccgcga ccggtgggct ggacgcgctg gtgctgaccg gcggagtcgg
      301 cgagaacgcg cccgacgtgc gggccgcgct ggccgcccgg ctgggttacc tcggcgtggc
      361 cgtcgacgcg ggtctcaacg ccagcgcgcg tcccgacgtc gacgtggctc ccgccgacgc
      421 gccggtccgg atgctggtgg tggccgcccg cgaggacctg gaggtggccc gccagacgcg
      481 ggctgtcctg cgctgagtgc ttggatgctg atcatgccca ccggaccagc cgccgccctg
      541 cccgagggtc tggccgaccc ggagctggcc gcctgggtcg ccgaggtgcg gcgggagccc
      601 ggcgagccgg tcggctcgat cggctcggcc cgcctgcggg aggcggcccg ccgccgggcc
      661 gccgaccggc cccgaggtcc cgagctggcg gtggtcaagg acctttgcac cgacaacggc
      721 atcgcgctgc ggctctatcg cccgacgctg cggccggcgc cgatggtggt cttcctgcac
      781 ggcggcggct tcgtcatcgg cgacctggac acccacgacg gcatctgccg ccacctcgct
      841 gccgacgccc gggccgcggt cgtggcggtg gaccaccgcc gcgccccgga gcatcccggc
      901 cctgcggccg tcgacgacgc ggtggacgcg gtctcgtgga cccgggcgca cctggtgaac
      961 ctcggcggcc tgtccgacgc cggggtcgcg ctcgccggcg acagctctgg cggagcagtg
     1021 gccgtgctcg ccgcctcgcg cctgcgcgag accgagacgt cgccgtcggc gctgctgctg
     1081 gcctacccca acgtcgacat gacgctgtcg atgcccagcg tcgagggcaa ggggcacggg
     1141 tggggcctgg actccgaggg cctgcgctgg ttcgtcgatc agtggctggg cgagtcaggg
     1201 cgaggcgatg acccccggct gagcccgtgg cacgccgacc tgtccggcct gccgccgacg
     1261 atcatcgcga ccgccgagca cgacccgctc cgcgacgagg gcgacgcgct ggcggcgcgg
     1321 ctgggcgagc tcggcgtccc agtgcaccac ctgccgcacg ccgggctggt gcacgggttc
     1381 ctcgggctcg ggcacgtctc ccccaccgcc gcgctggccg ggcacgcgct gttcgcgcgc
     1441 ttcggtgacc tgctgcggga gctcagcggc ccggcccgag gtcaggcggc gacgaagacg
     1501 gccagcagcg tgtagaccac cggcgcggtc ggcagcagcg agtcgagccg gtccatgagc
     1561 ccaccgtggc cgggcagcaa cgaacccatg tccttgaggc caaggtcccg cttgaccatc
     1621 gactccgcga ggtcaccgag cgtcgccgtg cacaccgccg ccaggccgac gagcacgccc
     1681 tgccaccaca ggccgtcgag cagcaggacg acggacaggg cgccgcccac cgcgcaggcg
     1741 gtcaccgagc cggcgaaccc ctcccacgac ttgcgcgggc tcaccgacgg ggccatcggg
     1801 tgacggccga gtgtcacccc tgccgcgtaa ccgccgacgt cgctgcacac ggtgacgagg
     1861 aagaagacca ccagccggac cggcccgtcg tcggcgcgca gcatcagcat cgtgaacccg
     1921 gccagcagcg ggacgtagag cacgatgaac gtgctggccg tgacgtcgcg gacgtagccc
     1981 gccacacccg cgggcagccg ccagaccagc accacgagca cggtcacggt tgccgccacg
     2041 agcagcccgt ccggcccggc gacgaaggcg gcgaccacca tgaccgccgc cccaaaggcg
     2101 atcgggacga gggcgacccg gggcccgccc gaggccaacg cggtcgccag ctcccagatc
     2161 gcgagcacga ccgcgatggc gaccagcccg acgaacagct ccttgcgcac gaacaacgag
     2221 cccagcacca gcccgccgag gaccaggccg acaccggtcg ccgcgacgag gttgcgcccc
     2281 gcgcgatcgt ggggctgcgt cggggccacg gtcacctcag acttcgagga gctcatcttc
     2341 cttgttcttg agcagatcgt cgatcgacgc gacgaagcgg gcggtgagct tttcgagctc
     2401 cttctcggcc cgaccggcct cgtcctcgcc gacctcaccg tcgcgggaga gccggtcgag
     2461 ggtctccttg gcccgccgac gcacgctgcg caccgagacc cgggcctcct cggccttgtg
     2521 ccgggcgacc ttgacgtagt ccttgcgccg ttcctcggtc agctcgggga acaccacccg
     2581 gatgcgctcg ccgtcgttgg tggggttgac ccccaggtcg gagtcgcgga tcgccttctc
     2641 cacggccgcg agcgagccct tgtcgtaggg ggccaccagg atgttgcggc cctcctgctg
     2701 gtggaaggac gccagctggt tcaccggggt gggcgcgccg tagtagtcga cgagaatctt
     2761 gttgaacatc gccggcgtca cacgtccggt gcggatggcc gcgaagtcct cccgcgccac
     2821 ggacagcgcc ttgtccatct tctcctcggc ctcgaagagc gtctcgtcga tcaccgggac
     2881 tccttggggt gtcagtcggt cgcgggcgca cgccgcggcg gcgtggtggt gtggtccgcc
     2941 tggctggtca ccagcgtccc gatcttctca cccgcgacgg cccgggcgat gttgccctcg
     3001 tcgagcaggt tgaacacgat gatcgggagc cggttgtcca tgcacaggct gaaggcggcc
     3061 gcgtcggcca ccttgaggcc gcgccggatc acctcggcgt agccgacggt ctcgaaccgg
     3121 cgggcctgcg ggtcggtgcg cgggtcggcg tcatagaccc cgtcgacgcc gttcttgccc
     3181 atgagcagcg cgccgcatcc gatctcgagg gcccgctggg cggccaccgt gtcggtggag
     3241 aagtagggca tcccggcgcc cgcgccgaag atcaccaccc ggcccttctc gaggtgacgc
     3301 acggcccgcc gggggatgta gggctcggcc acctgcccca tcgtgatcgc ggtctgcacc
     3361 cgcgtctcga cgccctgctt ctcgaggaag tcctgcaggg cgaggcagtt catcacggtg
     3421 ccgagcatgc ccatgtagtc agagcgggcc cggtccatgc cgcgctgctg gagctcggcg
     3481 ccacggaaga agttgccgcc ccccaccacg atggccacct gcgacccggc gcggaccacg
     3541 tctgcgatct gacgcgcgat gctctgcacg acgtcggggt cgatgccgaa ccggccgccc
     3601 ccgaacacct cgccggagag cttgagcatc acgcgtgggt agccggcgcg cggccagccg
     3661 ttgccatcgg cgtgagtcgc cggtgccggt gccccgtccg ccgcctcctg ggccgggatc
     3721 gccccggtca cggttccgtc cacggcaccg acctcctcgt cgtacgcgtc actgccgatc
     3781 gtggcacctg cctccggcgt tggtgggccg accgagtgct cagctggctc cgacctcgaa
     3841 gcgcgcgaag cgcgtgacct tgacgccggc cgcgtcgagc agcgccttca ccgacttctt
     3901 gtggtcctgg acgctggcct gctcgagcag gacgacatcc ttgtagaacc cgttgacccg
     3961 accctcgatg atcttgggca gggcgccctc gggcttgccc tcctcacgag cggtcgcctc
     4021 ggcgatgcgc gcctcgttgg ccacgacctc ggcggggacc tcctcacggg cgacgtactg
     4081 cggccgcatc gcggcgatct gcatcgcggc accacgggcc acgtcgaggt cgtcgccctc
     4141 gaacgcgacg agcacgccca cctggggcgg caggtcgctg gcgcgcttgt gcatgtaggt
     4201 cgccacctgg ccctcgagca ccacgacccg gcgcagctcg agcttctcgc cgatcgtggc
     4261 ggccagcgag gtgatgctct cggcgacggt gcgcccgtcg ggcagcgcct gggccagcag
     4321 cgtctcgacg tcaccgatct tggcggccgc cgcgagagcg acgatctcgg aggccagcgc
     4381 ctggaacgac tcgttcttgg cgacgaagtc ggtctcgcag gccagctcga tcatcgcgcc
     4441 gtcggtggag gccaccagac cgttgctcgc ggtgcggctc tcgccacgct tggctgcctt
     4501 ggccgcgccc ttgatgcgca gctcctcgac ggccttgtca aagtcgccgt cggtctcctc
     4561 cagggcgcgc ttggcgtcca tcatcccggc gccggtggcg tcacggagcc gcttgacgtc
     4621 tgcggcggtg aagttggcca tggtctcgct ctcttcgggt ggtgtcgatc agggtcaggc
     4681 aggtcgtgga cacgcagcgg cagtggcacc ggagcctcgc tcgggcgcca ctgccgcaac
     4741 gctcgatcag gcctgcgtcg cctcgggctg ctcgccttcg ctggcctcgg ccggcgcggc
     4801 ctgcgcctgc ggggcctgct cggcagtcgg agtcgcctca gccggggctg cctcagcagg
     4861 ggcggcctcg gccgaggcac cggccagcag ctcctgctcc cactcggcca gcggctcgtc
     4921 ggtcgccacg ccctcggccg gcgcctcctt gccggcaccg gcccgggcca tcagcccgtc
     4981 ggccaccgcg tcggcgagca cccgcgtgag cagggtgacc gagcggatcg cgtcgtcgtt
     5041 gcccgggatc ttgtagtcga cctcgtcggg gtcgcagttg gtgtcgagga tcgcgacgac
     5101 cgggatgccg agcttgcggg cctcgttgac ggccaggtgc tccttcttgg tgtcgatcac
     5161 ccacaccgcg ctgggcaccc gggacatgtt gcggatgccg ccgagggtct tctcgagctt
     5221 gtccttctcg cgcttgagga cgagcagctc cttcttggtg cgtcccgaac cggcgacgtc
     5281 gtcgaagtcg atctcctcga gctccttgag ccgctgcagg cgcttgtgca ccgtctggaa
     5341 gttggtgagc atgccgccga gccagcgctc gttgacgtag ggcatcccga cccgggtggc
     5401 ctgctcggcc acggtctcct gggcctgctt cttggtgccg acgaacagga tcgtgccgcc
     5461 gtgggcgacc gtctgcttga cgaagtcata ggcgcggtcg atgtaggaca gcgactgctg
     5521 caggtcgatg atgtagatgc cgttgcgctc ggtgaagatg tagcgcttca tcttggggtt
     5581 ccaccgacgg gtctggtgcc cgaagtggac gccgctctcg aggagctggc gcatcgtgac
     5641 gacggccatg ccgacgtgct ccttccgcgt ccgcgccgcg gcccgcaggg ccggcgccgg
     5701 cggacgcctc tcggttcctg gcgggtcacc ggccggcgcc cgcccctggc gcccgagcca
     5761 gcatcaccgt ggccccgacg agcgggacgc cacggaccga gatgcttgcc cacgccgtac
     5821 ggcgtatgag gcgcgcgagg tcgaccggct cacaccggtc gccaaccggc aatgttaccg
     5881 cacggcggcg gacccgctcc tggatcgagc gggtgggggc ctaccctgac cggtcatggc
     5941 gaccgggcgg ctcgtggagc gcatgcagtc cttcggcacg acgatcttcg ccgagatgtc
     6001 ggcgttggcc cgcgcgcacg atgcggtcaa cctcgggcag ggcttccccg acaccgatgg
     6061 gcccgacgag gtgctcgagg cggcggtggc tgcgatccgg tccggtcaca accagtaccc
     6121 gcccggccgc ggcatccccg agctgctcga cgcggtggcg gcccaccaga agcgctggta
     6181 cggcgtcgat ctcgacccca ccaccgaggt gctgatcacc gccggggcca ccgaggcgat
     6241 cgcggcgagc ctgaccgccc tggccggccc gggcgacgag gtgctcatgt tcgagcccta
     6301 ctacgactcc tacgccgcct gcgtggccct ggggggagcc acacgccggt gcgtgacgtt
     6361 gcgggcgccg gacttcgcgg tcgacctcga cgagctgcgg gccgcagtca gtccgcgcac
     6421 ccgcgtcgta ctgctcaact ccccccacaa cccgaccggc aaggtcttca cccgcggcga
     6481 gctggaggcg atcgccgccg tctgcatcga gcacgacctg gtcgcgatca ccgacgaggt
     6541 ctacgagcac ctggtctacg acggtcagca cgtgccgctg tcgaccctgc ccggcatgcg
     6601 cgagcggacc gtgacgatct cctcggcggc caagacgttc tcggtgaccg gctggaaggt
     6661 cggctgggtg agcggcccgg ccgagctggt cagcgcggtg ctcacggtca agcagttcct
     6721 cacctacgtc aacgcggccc cgctgcagcc ggcagtggcc gccgcgctgg cactgcccga
     6781 cgcctacttc gaccagctgc gcgactcgca ccgtgaccgg cgcgaccggc tcgtcgccgg
     6841 gctgcgcggc gccgggctca cgacgttcgt acccgccggc acctacttcg tcaccaccga
     6901 catcgcctcg atcggcgagc gcgacgggct ggcgttctgc cgcgagctgc ccgcccgcat
     6961 cggcgtggcg gcggtgcccg aggcggtctt ctacgacgac acggaggcgg gtaggtcgct
     7021 ggtgcggttc gcgttctgca agcggcccga gctgatcgac gagggcgtac gacgcctcgc
     7081 cgggctggcg acggctcggg ccggccagcc gggctgaccg ccggccatcc acaggctgcc
     7141 cgtcggcctg atccgtccac aggcacagtg ggctgtctgt ctcgctgccg gtcgcaagcg
     7201 gcaccgtcgt cgggtgcgca ccaccagtcg aaccctgctc gctgcgttgc tcaccgtggc
     7261 cctgacggtg ctcggcggcg gcccgacctg ggccgacgcc accgcgccgg tcccgcctgg
     7321 ccccgaccgg tgggccgcgc cggtgcagcc cctccggctc gcccgcgggt tcgcgccgcc
     7381 ggcccagcgc tggctgcccg gccatcgcgg catcgacctc gccgcgagcg tcggtgccga
     7441 ggtgcgggcc gtcgacgcgg gggtggtcgt gtgggccggt gacctcgccg ggatcgccgt
     7501 cgtctcgatc gcccacccca acggcctgcg ctcgacctac cagccggtcg aggcatccgt
     7561 cgagatcggt gacctcgtcg cgcgagggca gccgctcggc accgtcacgg tcgcgggcag
     7621 ccactgcgcg ccggccgcgt gcctgcacct gggcgccaag cggggccaga cctacctcga
     7681 cccgtcgctg ctgctggggc tgcgccgacc acggctgctg ccctacctga cgctggaccc
     7741 cacgggagca accggcgtgg cgccgaccgc gccggacacg cctgtcacgc ccgccgcctc
     7801 acccggtctg gtcgacgcgg tgtcggacgt gctcgcctcg atgctggctg cgctcggcca
     7861 agcggcgttg acctgaccgc ctccggtcac gcggcggcgt cggccagcag cgtgggcagc
     7921 cgccgcgcca gcacctcgta gtcggccggg cagttgtaga cctgggccga caggcgcagc
     7981 agtccctggc cgcgaaacgt cgtgacagcg acctcgaccc cggcatgctc ggcgatgcgc
     8041 cgcttccagg cctctgcccc ggccaggtcg acggcgacac cgggcggcag ggccaccagg
     8101 gccatcgaca acgtcgaccc atcgctcgtc ggaagcacgg cagcctcagg cacctgctcg
     8161 gacaccgcgg tggcgagcgt cgcccgaccc tgctgcacca gccgtcggtt gtgctcacgc
     8221 acccggtcga gacccagctc cttcatcagc cgcagggcgg cgggggcggc cagccacgcc
     8281 gtcaggtcgt ccgtgcccgt gcggtcgaag gccccgggaa agcccagatc gtcgttccat
     8341 gaggcgacca gcgggcgcag gccggtgcgc cgggtcggtg cggcgtagag caccgcggtg
     8401 ccccggggag cccaggccca cttgtgcagg ttgccggtcc agtagtcggg ggccagtcgg
     8461 gccaggtcga catcgagcat gccgggagca tgcgccgcat cgacgcagac ggcgacgccg
     8521 cgctcctgaa gcgccggcac cagccgatcg agcggcatcg cgcgtgcggt cgccgacgtg
     8581 acctgatcga tgaccgccag ccgagtccgc ggggtgacgg cggccagcac ccggtcgacg
     8641 atctcatcgt ccgtgaccgc cagcggcacc gcggccagga ccacccgggc accggcgcgg
     8701 gcgcaggccc ggtcggccgc gatgcgcacc gcgccgtagc catgctcggt caggagcacc
     8761 tcatcgccag gctgcagccc ggcgcaggcg aaaaccgtgt tgacgccggt cgtcgcgttg
     8821 cgcacgaggg cgagctcatc agcgcccagg ccgaagaact gcgcgatctc gccgcgggcc
     8881 gcgaccagcg actcggtcac cccacgggtg aagaaccgtt gcgggttggt ctccatccgg
     8941 tcgcgccacc ggtgctgctc ctcgagcacc ggcgtcggca cggctccgaa cgacccatgg
     9001 ttgaggtggg tcattgccgg gtcgagccgc cacagcgcgg cgacctgagc ccagtcggaa
     9061 ccccaggccg ggttggtccg ggagcccgcc gaggagtcga cggtctcggt cgtggatcgc
     9121 atcatcgatc agtcgtggtt gtcggcgagc cggctgcgca gctgcagcac ggccttggtg
     9181 tgcatctggc agatccggct ctcggtgacc ccgagcacct ggccgatctc ggccagggtg
     9241 aggccctcgt agtagtagag cgtgacgacg atcttctccc gttcgggcag gccgttgatc
     9301 gccttggcca gcaggtactt ggtctcctcg gactcgaagg ccaccactgg gtcttcggcc
     9361 ttggtgtcct cgagggtgtc gacgaggctg agcttgtcgc ccttctcgcc gctgacgctg
     9421 agcagctcgt cgagcgcgac cacgttgacg aacgagacct ggctgaagat gtggtgcagg
     9481 tcggagagcg cgatgcccat ctcggcggcg acctcggcct cggtcggcgt gcggtgcagc
     9541 tgggcctcca gcgccgcgta ggccttctcg acgtcgcgcg ccttcgagcg caccgaccgg
     9601 ggcacccagt cgatcgtgcg cagctcgtcg atgatggcgc cgcggatgcg ggagatggcg
     9661 taggtctcga acttgatggc gcgctcgagg tcgaacttct cgatcgcgtc gatgaggccg
     9721 aagatcccgt aggagacgag gtcggcctgc tcgatgttgg gcggcaggcc gacgccgacc
     9781 cggccggcga cgtacttgac cagcggggag tagtgaagga tcagccgctc gcgcagcgtg
     9841 ggatcgccgg agtccttgaa gtcgcgccag atttggcgca gcaccatctc gttgtgcgcg
     9901 acctcgtcga ccgcatcgtc gacgccgggc ctgctgaccc cgacctcgtc gggcttcacc
     9961 ggcgattccc ccgtgtcgtc tgctctgggc gcggcctcgg ccgggagacc ggctccgacg
    10021 tcgatgacga cgtcggtggc gtcggcaagc ccggtgctcc cccgctgggg gcgcgccttg
    10081 ctacgccctg gggtgcgcga gctcatacgt cgccttcaac cgctcgacag agacatgcgt
    10141 gtagatctgt gtggtcgcga gcgtagcgtg cccaaggatc tcttggacgc ttcgcaggtc
    10201 cgcgccgccc tccagcaagt gagtcgcggc gctgtggcgc agcccgtgcg ggcccaggtc
    10261 gggcgccgac tccaccgtct gcacgagccg gtgcaccacg gagcgaacga cgcgggggtc
    10321 gacccgcccg ccgcgcgcgc cgaggaacac cgcgggtccg gaccggccgg tgcacaggtg
    10381 ggggcgccct cgcgagagcc accggtccac ggcgcgcaag gcgggggcgc cgaccgggac
    10441 ggtgcgttcc ttcgcgccct tgccgaacac ccgcacgacg tggcgcgtcc ggtcgaggtc
    10501 gtcgacgtcg aggccgacga gctcgcccac ccggatcccg gtggcgtaga gcagctcgac
    10561 catggccagg tcgcgcagcg ccaccgggtc gtcgtcgtgg gcctgctcgg cgcacgcgtc
    10621 gagcagggcg gtcgcctcgg cgcggtcgag cacgtcgggc agggcgcgcc gggtcttgag
    10681 ggtgccgagc agcagaccgg ggtcgctgac gaggtgcccg tggcgccggg cccaggcggt
    10741 gaacgagcgg accgccgcgg ctcgccgggc gatcgtggcg cgagccgccc cgccggtgct
    10801 cgatcgcgcc agccagctgc gaagcgtcga cacgtcgatc tcggtcagct gggcgcgccc
    10861 catgcgctgc gcgtgctcga gcatgctggc cacgtcgctg cggtaggcgc gcaccgtgtg
    10921 ccgggagcag ccccgctcgg cccccaggtg ccgggcgaac tcgtcgagca tgccggccag
    10981 ctcatcgggc agcgccggcg tcggcgcctc gggtcgctcc ggcctgggca tgggggtcac
    11041 gctggtgccc gcgagcggcc gggtcaagca cccgggcgtc gcaggccgtc tgtgtcgtca
    11101 gaacagcgtc gccccgacct gccgggccga gccaggctcg tcgcgccgcg gtcggcaccg
    11161 gcgccagagc ccggaccggt gctcggccag cccggcgagc tcgagccggg tcagggccgc
    11221 gtgcacgctg agcagctcga gcccggccac ccggctgatc gacgcggcct cggccccctg
    11281 ccggaccggc agtgcctcga ccacccgccg ggtcagctcg tcgagcccgt cgagcggtgt
    11341 ctcggggccg cggccctcaa cgaccgcgtc gacgcccatc tgcccgacga cctcgagcac
    11401 gtcggcggcg ccacggacga gaacggtgcc aggtcggcgc agcagggtga gacacccgtg
    11461 cgactgctgg tcaccgaccc gagccggcac cgcacccacc caccggccca gggcgtcggc
    11521 gaagctggcg gtcgtcaggg cgccgctgcg gtgcgccgcc tcgacgacga ccgtggcctg
    11581 ggccagcgcg gcgatgaccc ggttgcgccg caggaaccgc acccgcgtcg ggctgcaccc
    11641 cggtgagagc tcggagacga cgagacccga ccgggcgatg cgggccagca gcccggcgtg
    11701 cgcacgcgga taggcgacgt cgacgccgca cgcgagcacc gccacggtcg gtccgtcgcc
    11761 ggccagggcg ccctcgtggg cgtggctgtc gatgccgtag gccgccccgg agacgatggt
    11821 ccagcctcgc tcggccagcg tggtgcacat ctcgtcggtg atgtagcagc cgccctcggt
    11881 ggcggcccgt gagccgacca ccgcgatcgc gcgctcggtg agctcgcgca ggtgtgcggg
    11941 cccgcgcacc cacaggccca gcgggcgtgc cgcatggctc atctgctggt cgcgcagccc
    12001 ggcgaggtcg tcgagctggt cgggccactc gtcgtcgccg ggtacgacgt aacgcgcccg
    12061 taccgcccgg gcgcgcgcca cgtctgctct caggtcgaga gtggccagcc gggccgacca
    12121 gtgcgccgcc gacgagccag gcagccgacg ctcgcgcaga cgagcgagca cctcgacggc
    12181 gccgagctcc tcgaccaggg cagccagccg gtcatcgccc ggctcggtca gccgggagag
    12241 ggcggcccgg gcccgtcgct ccgccgggtc aacgctcatg ccgctccccg ttcacgccgc
    12301 cacccggtgc gcggaccgca gcgctgcggc ggcatagacg tggtcggccg agggggccgg
    12361 gagcccggcg aggtcggcca gcgtccaggc cacgcgcagc acccggtcga cgccgcgggc
    12421 ggtcagccgc cccgcctgca gcagggcgtg ggccggcgcg gcgacatcgg gaggcaccgg
    12481 ccagcgtcgg cgcagcacgt agccggggat ctctccgttg cagcgccacc cggcacccgt
    12541 cgacccctcg tcgcggccga gccggtgacg catccgctca cgggcggccg ccacccgctc
    12601 ggcgaccacc gtgctggcct cgccccggcg ctcggcggcc agctcggccc ggctgaccgg
    12661 cggcagctcg acctgcaggt cgacccggtc gagcagtggc ccggacagct tgctggcgta
    12721 gcgccgcttg gccgcggggg tgcaccggca ggtctctccc cggctggcgc tgagcccgca
    12781 cgggcacggg ttggccgcca gcacgagctg gaaccgggcc gggtagcggg ccgtcgcgct
    12841 ggccctggcg atgacgacct cgccggactc gagcggctgg cgcagcgcct cgaggacgtc
    12901 ggccgagaac tctggcgcct cgtcgaggaa gagcacgccg ttgtgggcca ggctcagcgc
    12961 gcccggtcgt gggtggccgg acccaccgcc cacgatggcg gcccgggagg cactgtgatg
    13021 cgggtcctgg tagggcgccc ggtcgacgag cggggcaccc ggctcgagca gaccggccac
    13081 cgagtgcacg gcagtggtct ccagcgccgc ctccggcggc agcgggggca gcagcccggg
    13141 caggcgctcg gcgagcatgg tcttgccggc gccgggcgga ccggtgagca gcaggtggtg
    13201 cccaccggcg gcggccacct cgaggacgtg ccgacccagc cgctggccga ccacgtcggc
    13261 caggtcacgg gccggcgcgg aaaccgacgg cgtcgaggga ctcgacggtg cggtcgcggc
    13321 gggcgtccac tcgccgctca cccagtcggc cagtgcgcgc agcgagggca gcgcaaccac
    13381 gtcgacgccg ggcaccagcc gggcctcagc cgccgaggcc tcggcgacga ccacccgccc
    13441 gatcccggca gcggcggccg ccatgaccgc cggcagcacc ccccgcaccg gccggagacg
    13501 gccgtcgagg gagacctcgg cgagcagcac cgcgccccgc aacggcgcgg gcccgaacgc
    13561 gcgcatcgcg agcaggatgc cggcggcgat ggccgcgtcg tagccggtgc cgtgcttggg
    13621 caggtcgccg ggggccaggt tgacggtgat ccgatgctgg ggccagtcga agccggagtt
    13681 gaccaccgcc gcgcgcaccc ggtcgcgggc ctccccgagg gcggtgtcgg cgagcccggt
    13741 gatgatcacc gcgggcaggc cggcgccgat gtgcacctcg acgtcgacga cacgcccctg
    13801 caggccgagc agggcgatgg tgcgggtgcg ggcgagggtc acggctcagc cgaccccgcg
    13861 cacgtgctcg atctgcgccg gtcccgagcc ccggcgcagc accccgacga catcgaggcg
    13921 gatctgcggc ggatggaccc catggtcggc cacccaacgc acggcgagcc ggcgcaggcg
    13981 ggccagcttg accgcggtca cggtctccag cgggtggccg aacccgagtc cggaacgcgt
    14041 cttgacctca cagacgacga gcgcgtcgcc gtcgcgggcc acgaggtcga tctcgcccag
    14101 ctcgcagcgc cagttgcggt cgagcacgac catgccctgg cccaccaggt gcgcctcggc
    14161 caggcgctcg ccgtactgcc ccaggacgtc cttggcccgc atatgaccac ctccgggcac
    14221 ccacggtggc cggtcgagcg gtcagcggcc ggccgaggcg gcgtccctgt ggacgaacgg
    14281 cgcgggtgcc ggggctgtgg acaacggtca ggagcgaccg aagtcgcgcc cgccgggcag
    14341 gtccttgggc agctcgaggt cgctcttggc cagctcctcg acgttgacgt ccttgaacgt
    14401 cacgaccttg acgttcttga cgaagcgcgc cgggcggtac atgtcccaca cccaggcgtc
    14461 gctcatggtg acctcgaagt agacctcgcc gtcggccgtg cgggcctgca ggtcgacgtt
    14521 gttggtcagg tagaaccggc gctcggtctc gacgacgtag gagaagagcc cgacgacgtc
    14581 gcggtactcg cgatagagct gcagctccat ctcggtctcg tacttctcga ggtcctcggc
    14641 gctcatggtc ggctctccgc cgggacctgc tcggccagca agtcgtcgag gccgatgtca
    14701 gcggtgaggt cgagcccgac gtcgaggcca acgtccatgc cgtcatcttc gcgcatcgcg
    14761 agcgcgcgca cgttccagct gcgccgatgc tgctcgcaca ccccgaagga ccgcatcgcc
    14821 tccaggtgct cgaccgaggc gtagcccttg ttggactccc agccgtaggc gggataggtg
    14881 tgggccatct cgaccatgag cgcgtcgcgg gtggtcttgg ccagcacgct ggccgccgcc
    14941 accgacgcgc accgaaggtc ggccttgaca agggtcgtca ccgaccgcgt cacggcctcg
    15001 tcggtctcat cggcctcggc gtcgtcaaac aggctcggcg gcaccgtcgg gcgggtcagc
    15061 cagtcgtagc tgccgtcgag cagcaccagc cgaggagcca ccggcaggtc ggcgaacgct
    15121 cgctctccgg ccagccgcag cgcgcacagg atgccgagct cgtcgatctc ggccgcgctg
    15181 gcgtgcccga ccgcccacgc ccgggcccac cggcgcagcc ggggcaccag ggcgccgcgg
    15241 gcgttggggg tgagcagctt gctgtcgcgc agcccgcgtg gggcggtggc cacggccagg
    15301 tcgacgacca cgacccccac ggagaccggc cccccgagcg ccccgcgccc cacctcgtcg
    15361 accccggcga cgagcagctc gccggactca cgcagcatcc ggcgctcgac ccgcaggctg
    15421 ggcaccaccg aactcgcgcc gccgacccgc ccggcgcgct ccgcgatgct cacgactgac
    15481 ccgctcccga caggttgacc ggcgtcgccg gtggcccatt gcgcagcagc tccgccagtc
    15541 gcaccggcag ctcggccggc gcgacgacga tcgactcggc cgccatcgcc tcgagtgtcc
    15601 accaccggtg accggaggtc gcacgccgct ccagctcggt gaagcccgac gtgtcgaccg
    15661 tgaaggaggg agcccgcacg aggaagtaga cctcgcgctg gtcgtaggtc tgcccgcaga
    15721 agctgaagcg ggcccggcga cgccagacca gggggccgag ctcgacaccg cgcaggccgg
    15781 tctcctcacg cagctcgcgc agcgcgcact ccgtgggcga ctcgccgggg tcgacgccgc
    15841 cacccggggt gaaccagaac ctggcctcgg ggcgatcggg gtcggcgccg tggaagagca
    15901 ggacccgctg ctgctcgtcg agcaggacca cccgggcgct gtcgcggcgg atcggcgcca
    15961 gccccgtgga ggtcatgcgg gtcaccttac ggtccggccg cgacctgccc gaacgacgcc
    16021 ggccgctcga cccgctggaa ccggtcgaac ggccagatca ccacgaagac ccggcccacc
    16081 acgtcggcca tcgggaccat cccgtgcagg gtctgctcct ggtgggcccg agagtcctgc
    16141 gacacctgtc gatgatcgcc catcacccac atctgcccgg ccggcacctt gacgtcgaag
    16201 ggcgtgtcgg agggctcgtt gccggggtag agatagggct cgctcaacgc gacgccgttg
    16261 acggtgacac gcccctggtc gtcgcagcac acgacgtggt cgcccggcag accgatgatc
    16321 cgcttgacga gatcgcccgc ctgctcggag ggcagcacgc ccaggccgac gaacacgtcg
    16381 tgggcgagct tggccagccc ctcgcgctgc ggaaggtcct cggggtgcag ccagccgccc
    16441 gggtcctcga agaccacgac gtcgccccgg tgaaggtcgt cgaaacgggt cgcgagcttg
    16501 ttgacgagga tgcggtcgcc cagctgcagg gtgttctcca tcgatcccga cgggatgtag
    16561 aaggcctgca cgagaaacgt cttgatcagc gcggacaggg ccagcgacag cacgaccacg
    16621 atggcgatct cgacgaccat cggccggcgg gcgggacggc gcggcaggac atggcgagcc
    16681 gccgggcgtc cggccgatcg ggggttgcgc ggggcgggga cggagccggt ggacgtcacc
    16741 ggatccatgc cgacctcgtc ggccccgcgg gtcccatcgt ggttcaccac cgaaccgtac
    16801 gacggagatc gcctccgcgc acgaacggca cgaagatcgc gcccagagcg aacgccggcg
    16861 gcgtgccgag gccggcccag tgcggaggcg gccacaccac ggcgaccgcc cgaccgagca
    16921 cccggtcgac cggcaccatg ccgccgccgg ggtccccgag gtgggcccgt gagtcctgcg
    16981 actgcgcgcg gtgatcgccc atcacccaca gccggccggg cgggaccacg acgtcgaagg
    17041 acatcgtcga cggccggttg cccgggaaca agtacgaagt ctcgtcgagg gcggtgccgt
    17101 tgaccgtcac ccgaccgtcg gggtcacagc agcggacccg gtcaccggcc accccgatga
    17161 cccgcttgat gaagtcgtgc tcgcccgggg ggccgagccc caccagatca agcaggtcgt
    17221 cggcgagagc gcgcaacccg ctggcgcgtc tcgcggcggc ctcgggcacg aacgagtcga
    17281 cgccgtcgaa gacgacgacc tcgccgcggc gcgggtcgcg cacgtgcagg ctgaccttgt
    17341 cgacgagcac ccggtcaccc acccgcagcg tcggctgcat cgacccggac gggatgacgt
    17401 aggcctggac caggaacgcc ttgacgagga tcgccacgac cagggcgagc agcaccagga
    17461 cggcgacctc gacccagacg ggccagcgtg acccgcccgg cccccgcggc gcacgcaggc
    17521 gcacggcgtg ctcgcccggg cgtccggcgg ggtcagcgac cggcggccga ggtgtcgcgc
    17581 ttctccttga tcttggcggc cttgccgcgc aggtcacgca ggtagtagag cttggcgcgg
    17641 cgtacgtcgc cccgggtgac gacctcgatc cgatcgatga tcggggtgtg cagcgggaac
    17701 gtgcgctcca cgccgacgcc gaagctgacc ttgcgcaccg tgaacgtctc gcggaccccg
    17761 ctgccctggc ggcggatcgc cacgccctgg aagacctgga cgcgggagcg gttgccctcg
    17821 acgacgcgga cgtgaacctt cagcgtgtcg cccggacgaa agtcgggcag gtcgtcgcgc
    17881 agcgaggcgg cgtcgacgga gtcaagcgtg tgcatggagg ttcgcttcct cgcaggtgcc
    17941 acaggtcacc cacgtgatcg gggtcagtgc gtgaaaggca cgccactcgc cggtgcagcg
    18001 ctccccctgt ggcaggggcg caactggtgc cgacggcgcg ccgacaacgg ctgatcagtc
    18061 tgccacagcg ggcgcagccg gggcgaatcg cgtcagccgg ccgtcggcgc ccggtgccca
    18121 gccgagcccg gccagcagct gcaggtcggc ggcgtccagg ccgtcgaggg gcagggccgc
    18181 gagcaggtcg ggccggcggg tcgccgtacg gcgcagggac tcgtcacgtc gccaccggtc
    18241 caccgcggcg tggttgcccg agagcagcac cgggggcacc tcgcgcccac gccagctggc
    18301 cggcttggtg tagacggggt actcgagcag ccccgagccc agcgcgtgcg actcctcgac
    18361 caacgactcg gcgttgccga tgaccccggg gagcagccgg gccaccgcct ccacgacgac
    18421 cagcacggcc acctcgccgc cgttgagcac gtagtcgccg agcgagacct cctcgacccg
    18481 ccagcggctg gccgcctcct cgaccacccg ggcgtcgatg ccctcgtagc gcccgcagac
    18541 gaacaccagc cgagacgcct gcgccagctc gtgcgccagc gcctgggtga acgggcgacc
    18601 cgacggggtg ggcacgagca cgaccggggc atccgccccg tcgccggcga cctcctcgag
    18661 ggcggccccc cacggctcgg gcttcatgac catgccggcg ccgccgccgt agggggtgtc
    18721 gtcaacggtg cggtggcggt cggtggtcca gtcgcgcagg tcgcgcaccc gcacgtcgag
    18781 gagcccctcg gccctggcct tgccgatcag ggagagcccc agcggcgcca ggtagtcggg
    18841 gaagatcgtc aggacgtcga tccgcacggc tagggcgccg tctcgaggtc gagcaggccg
    18901 gcgggcgggt cgagcaggac gtgcccggcg gccaggtcga cagcggggac gatggcactg
    18961 acgaacggca cgagcacgtc ggcgccttcg cgccgtacga cgagaaggtc ctgcgcgccg
    19021 ccgtgctgga ccgcgaccac ctcgccgacg tcctcgccgg ccacggtgcg ggcccggagc
    19081 ccgaccagct ggtggtcgta gaactcgtcg gggccggcgg cctcgtgcgg gtcgacctcg
    19141 gcgagcagca gggtcccgcg cagcgcctcg gcggcggtgc ggtcgcccgc ctgcgcgaac
    19201 gccaccacga gccggccgga gtgctcgcgc gcgtgcgtca ccgtcagcgg accgctgctc
    19261 gcgggctcgg tggccagcac cgcgccggcg acgaagcgtt ggtcgggcga gtcggtgcgc
    19321 gagtcgacga gcacctcgcc gcgcacgcca tgggcccggc cgatccgccc gaccacgacc
    19381 tgcatctcga ccccgacggg gctagcgctc gtcgacgtcg aggaagtcca cccgtacgcc
    19441 gcggccgggg ccgagcgccc cgatcacagt gcgcagcgcc ttggcggtgc gtcccccccg
    19501 gccgatgacc ttgcccaggt cggaggggtg cacccgcacc tcgagcactc ggccccggcg
    19561 cagggcacgg tcgcggacct tgacgtcgtc agggtgctcg acgatgccct tgacgaggtg
    19621 ctcgagcgct tcctcgagca cggtcagctc tcgccctggg tctcggcggc cttcgcctcg
    19681 tcggacgcag gcgcctcggc ggccggctca gccttggcct cgtcgggctt gggctcggcc
    19741 ttggcggcct tcttcttcgg agtcgtggcg gcggccttgg gctcgccgag cgtctccttg
    19801 gcggcggcct cgaagatggc cttcttgtcg gcccgcgggg cggcggtctt cagcgtgccc
    19861 tcggcgccgg gctcaccccg gaacttctgc cagtcgccgg tgaccttgag gatggccttg
    19921 accggctcgg tcggctgagc accgacgccg agccagtact gcacccgctc gctcttgacc
    19981 tcgatgaacg agggctcctc ggtcgggtgg tacttgccga tctcctcgat cgcgcgtccg
    20041 tcccgcttgg tgcgggcgtc ggcgacgacg atgcggtagt agggggcacg gatcttgccc
    20101 atgcgaagaa gcttgatctt gacggccacg agtgtggtga ctcctgtgtc gtgaggatgg
    20161 gcctgcgcga tcgcgtgggg actgcgctcg ctcggggccc ggcggacacg ccgtgcgcgg
    20221 gagagagggc cgcacgcacg ccgagtacgg tcggtcattg tgccagaccg cggaggcacc
    20281 cgcgaaccat gcgccgcggc agccctgccg tcgacccgat gaccgggtca gcgggtggga
    20341 gcgacgaccc gaccccgcag cacgatcgtg cgggggcggc gcagcgtcgt gaggtcgagc
    20401 cgggggtctt cgtcgtagac gacgaggtcg gcgggcgtcc cctcgtcgag gcgcgccggt
    20461 agccccagcc aggcgcgggc ggcccaggac ccggcggcca gcgcctcggc cgggctcatc
    20521 ccgaacccgc acatcgccgt gatctcctcg gcggccagcc cgtggggcag caccccaccg
    20581 gcgtcggtgc cgcagtagat cggtacgccg gcctcgtggg cggcccgcag cgtggcgtcg
    20641 acgcccgcgt gcaggtggcg catgtgcgcg ctgtagcgcg ggaacttgtc ctccccggcc
    20701 gcggcgtagt gcgggaagtt gtcgatctgg atgcgggtcg gcacgagggc cgtcccccgc
    20761 tcggccatca tcgcgatcgt ggcatcggtc aggccggtgc cgtgctcgat gcagtcgatg
    20821 ccggcgccga gcaggtcggg cagcgcgtcc tcgccgaaga cgtgggcggt gacccgcacg
    20881 cccagctcgt gggcccggcc gatccccgcc tcgagcacct cacgtggcca gcagggggtc
    20941 aggtcgccga cctcacgctc gatccagtca ccgacgagct tgacccagcc gtcaccgcgc
    21001 ttggcctgct cctcgacggc ggccaccagg tcggccgggt cgacctcgac gccgtagttg
    21061 cggatgtagc gccggctgcg cgcgatgtgg cgcccggccc ggatgatgcg gggcaggtcg
    21121 tcgcgctcgt cgatccagcg ggtgtcggcg gccgagccgg cgtcgcgaat cagcagcgtg
    21181 ccgacgtcga ggtcggcacg ggcctgctcc tcctgaacgt cgggaggcac cgcgccgttg
    21241 gcgtcgagcc cgacgtggca gtgcgcgtcg acgagccccg gcatgatcca gccgccgtca
    21301 acgacggtct gcgcaccggc gaccggctcg gtcgtgacca cgccctcgcg cacccatagg
    21361 tcgcgctgct ccccctcagg gagtacgacg ccacgcaggt gcagcgtctt cgtgggcgcc
    21421 cggccgctgg aagcatccat ggcagccgag gctagtggcc gtcgggcggc agcatctcgc
    21481 gcagggcggc cggcagctcg aagtcggccg gcagcgcggg catcgacgcg tcgcccgacg
    21541 cgccctcgat cgcgcgctcg gcggcggccc gcttggccgg gttgcccgac cgagcgccct
    21601 tgccgcgctt gcccgccgcc ttgcccttgg ccttcttgct gcgcccgccc ggcccggcca
    21661 tgcccggcat ggccggcatc cccggcaggc caggcatccc ccgcagcccg cccttgccca
    21721 tctggcgcat catcttctgc gcctgcccga agcgttcgac gaggttgttg acgtcgctgg
    21781 tctggcagcc ggagccccgg gcgatccgca gtcggcgcga cccgttgagg atgcgcggat
    21841 cggcccgctc ctgcggggtc atcgacttga tgatcgcggc gacccggtcg atctcgcgct
    21901 cgtcgatgct ctcgagctgc tcgcgcacct gccccatgcc cggcagcatc ccgaggatct
    21961 tggacagcga gcccatcttg cggacctgct ccatctgcgc gaggaagtcc tcgagcgtga
    22021 agccgtcgcc gcgctggagc ttgctggcca tcttggccgc ctgctcctgg tcgaacgtct
    22081 tctcggcctg ctcgatcagg gtgagcatgt cgcccatgcc gaggatgcgc gaggccatcc
    22141 ggtcgggatg gaagacgtcg aagtcctcga gcttctcgcc gttggaggcg aacatgatcg
    22201 gccggccggt cagctcgcgc accgagagcg cggcaccacc gcgagcgtcg ccgtcgagct
    22261 tggtaaggac cacaccggtg aagtcgacgc cgtcgaggaa cgcctgtgcg gtcgtgacgg
    22321 cgtcctggcc gatcatcgcg tcgaggacga agagcacctc gtcgggcgag acggcggccc
    22381 ggatgtcggc ggcctgagcc atgagctcgg cgtccacgcc gagccgaccg gcggtgtcga
    22441 cgacgacgac gtcgaagagc ttggtccggg cgtgctcgat cgaggctcgg gccaccgcca
    22501 cggggtcgcc gacgccgttg cccggctcgg gggcgaagac cgtgacgccg gcccggtcac
    22561 cgaccacctg cagctgggtg accgcgttgg gccgctgcag gtcggcggcg accagcagcg
    22621 gctggtggcc ctggcccttg agccagagcg cgagcttgcc ggccagcgtg gtcttgccgg
    22681 cgccctgcag gccggccagc atgatcacgg tggggggcgt cttggcgaag gtcacccgcc
    22741 gggtctgccc gccgaggatc tcgacgagct cctcgtcgac gatcttgacg acctgctggg
    22801 ccgggttgag cgcctgggag acgtcggcgc ccagcgcccg ggtcttgacc ttgccgatga
    22861 acgagcgcac caccggcagg gcgacgtcgg cctcgagcag ggcgacccgg atctcacggc
    22921 aggtggcgtc gacgtcggcc tcggtgagcc gacccttgcc gcgcagcccc ttgaaggtga
    22981 cggccaggcg gtcagaaagc gtctcgaaca cggtgaggtg ggtcctcgct gatgggcgta
    23041 cggcggtcgt tgcggacccg gtcagggtaa ccgagccact ggtcctcgct gggcgagcgc
    23101 cgtgcccggc acaggcgcga gcgcgtaggc cgactcggcg tggacggcga ggtcgatgcc
    23161 gacccgctcg tcggcctcgt cgacccgcag gccgatcgtg cggtcgatga cgctcccgat
    23221 gacccaggcg agcgcgaacg agtagccggc caccacgagc gcggccaccg cctgacgagc
    23281 gagctgggac cactgcccgc cgtacagcag cccggccgga cccccggtga tcgccccgga
    23341 ggcgagcagg ccgaccagca gggtgccgac catgcccccg accaggtgca cgccgacgac
    23401 gtcgagggag tc
//
LOCUS       DATIBB010000128         2065 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_601294, whole genome shotgun sequence.
ACCESSION   DATIBB010000128 DATIBB010000000
VERSION     DATIBB010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_601294"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..219
                     /locus_tag="VLC50_04905"
     CDS             <1..219
                     /locus_tag="VLC50_04905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF26840.1"
                     /translation="DLLRERHVSASDLVPALVAEGVATEDEIRGSIADPGITGAVQMV
                     DAVLARASRPAAAGPADAEAPGVDGRVS"
     gene            219..1193
                     /locus_tag="VLC50_04910"
     CDS             219..1193
                     /locus_tag="VLC50_04910"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HSF26841.1"
                     /translation="MLPRFDLGVRPTPLVRAHRLEDALGTGPVLLKRDDLIGFAGAGN
                     KARPLELIVGAALEEGADTLVTGGSAGSNFVHAAACAARVAGLAAELLIAGPTPLPAA
                     PGLALAAAAGARIIATGGPREQVDAAIAERAEVLRAAGRRPMAVPRGGSTPLGAVGFA
                     LAVAEVAAQLDALDVAPALVVIALGSGGTTAGLLAGLAANRLSWPVLTVSVSRPVEAI
                     TAKVLTLAQQCADLLGAGPIDPGLLEVVDARDPAHGGTRGPATEAITLGLATEGLLLD
                     PVYSGPAFGEAVHRARAGLDGPLLFWHTGGLLPAVAALADTPHKEYAA"
     gene            1190..>2065
                     /locus_tag="VLC50_04915"
     CDS             1190..>2065
                     /locus_tag="VLC50_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007581501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HSF26842.1"
                     /translation="MNGHRRQGPPQSGPARELIESGFAIENEDAPFLHAGLNLADLAH
                     VLDLRRRGIVPEDAARALLELLLEVHQMPVEDFPYDPAYGEPYNSREHYFVSRLGDVA
                     GWMHAGRPRREAARIAMRLHLRRQLLALVTEVVAFAETLTTRAGEHLDTVMPDQTYLQ
                     QAQPSTFAHYLLSFVYPALRDADRMIDELGWVNASPGGAGCVNGTRLQDDRTHVARAL
                     GFDGIIVNTRDAMWQVDGLIHILAAGASMLSNFSKLGEDLEIWSSSEFDFVDLADSYS
                     RASVLMPNKRNPYSLS"
BASE COUNT          248 a          820 c          731 g          266 t
ORIGIN      
        1 gacctgctgc gcgagcgcca cgtcagcgcc tccgacctgg tgcccgcgct ggtcgccgaa
       61 ggcgtggcca ccgaggacga gatccgcggc tcgatcgccg acccaggcat caccggcgcg
      121 gtgcagatgg tcgacgcggt cctcgcgcgc gccagccggc cagccgcggc cggcccggcc
      181 gatgccgagg cgccgggcgt tgatgggcgg gtctcgtgat gctgccgcgc tttgacctcg
      241 gggtgcgccc gaccccgctg gtgcgggccc accggctcga agacgcgctg ggcaccggcc
      301 cggtcctgct caagcgcgac gacctgatcg gtttcgccgg cgccggcaac aaggcgcgcc
      361 cgctcgagct gatcgtcggc gccgcgctgg aggaaggcgc cgacaccctg gtcaccggcg
      421 gcagcgcggg gtccaacttc gtgcacgccg cggcctgtgc ggcccgggtt gccgggctcg
      481 ccgccgagct gctcatcgcc gggccgaccc cgttgccggc cgcccccggc ctggcgctgg
      541 ccgccgcggc cggcgcccgc atcatcgcca ccggcgggcc gcgcgagcag gtcgacgccg
      601 cgatcgccga gcgggccgag gtgctgcggg cagccggccg ccggccgatg gcggtgcctc
      661 gcggtgggtc caccccgctg ggcgcggtcg ggttcgcgct cgcggtggcc gaggtcgccg
      721 cccagctcga cgccctcgac gtcgcgccgg ccctggtcgt catcgcgctc ggctcgggcg
      781 gcaccaccgc ggggctgctg gccgggctgg ccgccaaccg gctgtcctgg ccggtgctga
      841 ccgtctcggt cagccgcccg gtcgaggcga tcaccgccaa ggtgctcacg ctggcccagc
      901 agtgcgccga cctgctcggt gccggcccga tcgaccccgg gctgctcgag gtcgtcgacg
      961 cccgtgaccc cgcgcacggc ggcacccgcg gcccggccac cgaggcgatc acgctcggcc
     1021 tggccaccga gggcctgctg ctcgacccgg tctactccgg gccggcgttc ggcgaggccg
     1081 ttcaccgggc ccgcgcgggc ctcgacgggc cgctgctctt ctggcacacc ggcgggctgc
     1141 tcccagcggt cgccgcgctg gccgacacac cacacaagga gtacgccgcg tgaacggcca
     1201 ccgccgtcag gggcccccgc agagcgggcc ggcccgcgag ctcatcgagt cgggcttcgc
     1261 gatcgagaac gaggacgcgc cgttcctgca cgccgggctc aacctggccg acctggccca
     1321 cgtgctcgac ctgcgccggc gcgggatcgt gccggaggac gccgcccgag cgctgctcga
     1381 gctgctgctc gaggtgcacc agatgccggt cgaggacttc ccctacgacc cggcctacgg
     1441 cgagccctac aactcgcgcg agcactactt cgtctcccgg ctcggcgacg tggccggctg
     1501 gatgcacgcc gggcgcccac gccgcgaggc cgcccggatc gcgatgcgcc tgcacctgcg
     1561 ccgccagctg ctcgcgctgg tcaccgaggt cgtcgcgttc gccgagacgc tgaccacccg
     1621 cgccggcgag cacctcgaca ccgtgatgcc cgaccagacc tacctgcagc aggcccagcc
     1681 ctcgacgttc gcgcactacc tgctctcgtt cgtctacccc gcgctgcgcg acgccgaccg
     1741 gatgattgac gagctgggct gggtcaacgc cagcccgggc ggggccggct gcgtcaacgg
     1801 cacccggctg caggacgacc gcacccacgt cgcgcgcgcg ctgggcttcg acgggatcat
     1861 cgtcaacacc cgcgacgcga tgtggcaggt cgacgggctc atccacatcc tggcggccgg
     1921 cgcgagcatg ctgtccaact tctccaagct cggcgaggac ctggagatct ggtcgagctc
     1981 ggagttcgac ttcgtcgacc tggccgactc ctacagccgg gccagcgtgc tgatgcccaa
     2041 caagcgcaac ccctactcgc tctcg
//
LOCUS       DATIBB010000129         8721 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_863736, whole genome shotgun sequence.
ACCESSION   DATIBB010000129 DATIBB010000000
VERSION     DATIBB010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 8721)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8721)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8721
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_863736"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..351
                     /locus_tag="VLC50_04920"
     CDS             <1..351
                     /locus_tag="VLC50_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015884219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HSF26843.1"
                     /translation="RAFTVWAVLRALGRTGVAELVNRLCRHAAAFGVGLAEIPDATVL
                     NDVAFTQVCASFGSDELTEAVVRRLLAEGTAWMSGSTWHDRSVLRISVSNWSTTDDDV
                     ARSLDAVRKAANGV"
     gene            complement(348..968)
                     /locus_tag="VLC50_04925"
     CDS             complement(348..968)
                     /locus_tag="VLC50_04925"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013772821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HSF26844.1"
                     /translation="MARYFDVHPKDPQPRSIAQVVGLLRDDALIAYPTDSCYALGARL
                     DSPTGAERIRRIRHLDDSHHFTLVCAEFAQLGQLVHMSNAAFRVVKASTPGPYTFILP
                     ATKEVPKRLAHPKKKTVGVRIPDHPVVRALLRELGEPLLSSTLLLPGTEDPMTDGWAI
                     KEELDHQVDAVIDAGDCGTEPTTVVDWSEGSPEVVRVGAGDPTRFA"
     gene            1016..1612
                     /locus_tag="VLC50_04930"
     CDS             1016..1612
                     /locus_tag="VLC50_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011690471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HSF26845.1"
                     /translation="MAIELPELLVPDAPAWRDWLLEHHASAPGVWLVLGKKGGTVTAL
                     TYDDALDEALCFGWIDGQVNRRDAGSYVQRMTPRGPKSSWSARNVEHVARLEREHRMT
                     EAGRAAVRAAQTDGRWDNAYGGPATAEVPADLAAAIAADPRAQAMFDVLTTTNRYAMI
                     YRINTVKRAETRARKIREYVEMLARQETLYPQRRRPAP"
     gene            1701..4013
                     /locus_tag="VLC50_04935"
     CDS             1701..4013
                     /locus_tag="VLC50_04935"
                     /EC_number="2.7.7.65"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HSF26846.1"
                     /translation="MTPLVTGKRARVGEIATWLGWRGWLVIGVLAVDGLLVVALPVTR
                     ASATLANVVLTGSAAVSALVCVRAGQRGPHRRAWWLMALALLAWAVGNLVWTLDLVVL
                     GRAPSGAGLADLFYVAFYPLAVAALVCLPGAPTSTVGRLRTVLDGMIAGAALVVLLWE
                     PLLAPLLRRAQTPLGHAVSLAYTVGDVALLAVLVSLAAHSTMRGRRAGALLIAGFALV
                     TIADTAYAVGVYQDSYVSGSPVILGWTFGFALLGLAASQPAGTTDPATSAVPPRRWRA
                     HVPLLLVAPALLLAAVTVSGRGALSTGMALPGLIGVVLVLGRQTLAVRENVELNHRLE
                     HLVAAKTASLTQLAYSDPLTGLPNRRRLIEELAAPGPADGSTQRWLGLGDVDGFKQLN
                     DTLGHLAGDDALRHIAHQLRLARGADDLVFRLGGDEFAVLLHAASAEAALHTANRLAQ
                     ALQEEALVAGRLLRLRASLGIAPVVAGPDQALRHADLAMYAAKQAGGGQVKLFDHTLT
                     DLAFARMDLEHDLREAVATGGLGVVYQPILDLATGAIVGAEALSRWTHPDRGVVSPDV
                     FIPLAEESDLISVLGANVLAAACRAAHDWRTRDGRPLPVWVNLSPRQIEHRHFVADVL
                     ELLATSHLDPHALVLEVTEGLFLDPAGTAATTLQQLRGAGVRIAIDDFGTGYSSLARL
                     HQLPVDILKLDREFVEALSRDTLVQPAITRAIIAMAASLNLTLVAEGVETEEQRALLL
                     REGARFGQGFGLGRPQSGTAFTALAPLTLR"
     gene            complement(4010..4219)
                     /locus_tag="VLC50_04940"
     CDS             complement(4010..4219)
                     /locus_tag="VLC50_04940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF26847.1"
                     /translation="MARATDEPDGLQGRQVFNGREWVDYTSLVPAPAKRPWLRWVVTG
                     IVALIVVAVFGYAVAASGEVGGMFG"
     gene            4389..5564
                     /locus_tag="VLC50_04945"
     CDS             4389..5564
                     /locus_tag="VLC50_04945"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HSF26848.1"
                     /translation="MRDGQAAAMRAAEDAVQAADPWLAAVRAAVAEPSIPDLHAQPIV
                     DLARGEVAGYEVLARFPGPPQAAPDEWFAVADRAGLAAYLEARVLDRALQLRSSLPRG
                     AFLSVNVSTHLLASEPVQQVLARAGSLAGTVLELTEPVPEQRPDLTNALCSVTAVGGR
                     IAIDDAGAGHHGMRQMATVRPALVKLDRSLVGGIDTDPTKAAVVEMLGDFANRLDARL
                     LAEGIETEAELRTLVGLGVPLGQGFLLARPDLPWVGLTEAVIALLRSEAHAGRDGHVA
                     ELVEDADVVGAGSGEAPQTGQIIVEVDRGEHPVAMRHATGGQLHRTTGPALLMVLPSD
                     RIEHVAHTALARSSPHRFEPLVCVDIAGRLVGVVRMERLVSALAARASDPLSAPGHS"
     gene            complement(5542..6435)
                     /locus_tag="VLC50_04950"
     CDS             complement(5542..6435)
                     /locus_tag="VLC50_04950"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HSF26849.1"
                     /translation="MSKHRRPRRSPVAALRPSALRPAVHPAALLRPALRSPALARSSK
                     TLLIAAVSGGLLLSSGIGAEAEPPEVDGLAAAAPSGSSTRTVAPAVVDLSRTDVVAYV
                     ASEAASTAEADRIIAAVELAKDRRAAAAKAAADKAAAAKAAAEAKAAKAAAAAKAAKE
                     AREAKARASRTATRATAPLPAGFGARVLAVAARYAGVPYVWGGSTPSGFDCSGYVGYV
                     FKQLGIDLPRTAAQIRAATDRITRSQLRPGDLVFVHKASGVSHVAIYAGSNMWWEANR
                     PGKPVGKHAAWTTSVSYGRVR"
     regulatory      complement(6436..6588)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00379"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-AMP (ydaO/yuaA leader) riboswitch senses
                     the signaling molecule cyclic di-AMP; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="cyclic di-AMP"
                     /db_xref="RFAM:RF00379"
     gene            complement(6689..7864)
                     /locus_tag="VLC50_04955"
     CDS             complement(6689..7864)
                     /locus_tag="VLC50_04955"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HSF26850.1"
                     /translation="MTESSGPEEPAGSSPAPPGSAARELASLGHYARVTVTVLAVVAV
                     ALLAYRARGVLILVLSGFLIAAGLDPLVAAVARRGLRRGLAVALVFGAGLLATALFVV
                     LALAPAIGQFAQVVTALPGQLQELGERLQGSGPISDWLAREDIREQAQTLLDRVPALL
                     ASSVGALFGAVTAVIGLIFAGFTVAALSVYFLLALPRIRRFAAAALGDPERAAVMSDA
                     LGRVGGYITGQLAICLLHGVCSYIVFVILDLPYAALLAVTVAILDAVPQVGALLGALV
                     ALVFAVPLGIVTSVAVVVYFVVYQQLENYVVAPRVFSRAVELSPLAVFVAVLVGATLT
                     GLVGAVAALPVTAAGKVVLRYVFRDRLARIEASAHGVDPEPGVTAAGADDGPAITVA"
     gene            complement(7869..8564)
                     /locus_tag="VLC50_04960"
     CDS             complement(7869..8564)
                     /locus_tag="VLC50_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014143142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HSF26851.1"
                     /translation="MSHDLIDTTEMYLRTIYELQEEGVVPLRARIAERLGQSNPTVSQ
                     TVARMERDGLLVVGGDRQLELTEEGQSLATRVMRKHRLAERLLVDVIGLDWEYVHTEA
                     CRWEHVISEAVERRLLELLDHPTESPYGNPIPGLDELGAKPSHDAFLEGVVALSEVEL
                     GAATTVVVRRMGEPVQTDSTLLSRLDRAGVRPGAAVQAAPSLQPGQFVLGSAGEETEV
                     SREVASHIFVVRR"
BASE COUNT         1157 a         3230 c         3194 g         1140 t
ORIGIN      
        1 cgggcattca cggtctgggc ggtgctgcgc gcactcgggc gcaccggagt ggccgagctg
       61 gtcaaccggt tgtgccggca cgccgcggcc ttcggcgtcg ggctggccga gatccccgac
      121 gccaccgtgc tcaacgacgt cgcgttcacc caggtgtgcg catcgttcgg gtccgacgag
      181 ttgaccgagg cggtcgtacg gcgactgctc gccgagggca ccgcctggat gagcggatcc
      241 acctggcacg accgcagcgt gctgcggatc tcggtgagca actggtccac caccgacgac
      301 gacgtcgcgc gcagcctgga cgccgtacgc aaggccgcca acggggtcta ggcgaagcga
      361 gtcgggtcgc cggcgccgac ccgcaccacc tccggtgagc cctccgacca gtccacgacc
      421 gtggtcggct cggtgccgca gtcgccggca tcgatcacgg cgtcgacctg gtggtcgagc
      481 tcctccttga ttgcccagcc gtcggtcatc gggtcctcgg tgccgggcag cagcagggtg
      541 ctcgacagca acggttcgcc gagctcgcgc agcagcgccc gcaccaccgg gtggtcgggg
      601 atgcgcaccc cgaccgtctt cttcttgggg tgggccagcc gtttgggcac ctctttggtg
      661 gccggcagga tgaacgtgta ggggccgggc gtcgaggcct tgacgacccg gaacgccgcg
      721 ttgctcatgt gcaccagctg gcccagctgg gcaaactccg cgcacaccag cgtgaagtgg
      781 tgcgagtcgt cgaggtgacg gatccggcgg atccgctcgg cgccggtcgg gctgtccagc
      841 cgggcgccca gggcatagca ggagtcggtg gggtaggcga tcagcgcgtc gtcacgcagc
      901 agccccacca cctgcgcgat gctgcgcggc tgcggatcct tgggatgtac gtcgaagtag
      961 cgcgccatgg gccgagggta cgggtgggcc aaccggcccg gaggggagca caggcatggc
     1021 gatcgagctg cccgagctgc tcgtgcccga cgcgcccgcc tggcgggact ggctgctcga
     1081 gcaccacgcc agcgcgcccg gcgtctggct ggtgctgggc aagaagggcg gcacggtgac
     1141 ggcgctgacc tatgacgacg ccctcgacga ggcgctgtgc ttcggctgga tcgacgggca
     1201 ggtcaatcgt cgcgacgccg gcagctatgt gcagcggatg accccgcgcg ggcccaagag
     1261 cagctggtcg gcacgcaacg tcgagcacgt ggcccggctc gagcgcgagc accggatgac
     1321 cgaggccggc cgggccgcgg tccgcgccgc gcagaccgac ggccgctggg acaacgccta
     1381 cggcggcccg gccaccgcgg aggttccggc cgacctggcc gcggcgatcg ccgccgaccc
     1441 gcgcgcgcag gcgatgttcg acgtgctgac caccaccaac cgctacgcca tgatctaccg
     1501 catcaacacc gtcaaacgcg ccgagaccag ggcccgcaag atcagggagt acgtcgagat
     1561 gctggcccgg caggagacgc tctatccgca gcgacgccgt cccgccccgt aggcgggccg
     1621 gggcggatcc ctcaagtccc gccggggcgt gccgatcgac tcggcatggc cgccgtgcgc
     1681 gacgctcgtc tgcagaggac gtgaccccgc tcgtcacggg gaagcgcgcc agagtcggcg
     1741 agatcgcgac ctggttgggg tggcggggct ggctcgtcat cggtgtgctc gccgtcgacg
     1801 gtctcctcgt cgtcgccctg ccggtgacgc gtgcgagcgc gaccctggcc aacgtcgtgc
     1861 tcaccggcag cgccgcggtc agcgcactgg tgtgcgtgcg ggccgggcag cgggggccgc
     1921 accggcgggc gtggtggctc atggcgctcg cgctgctcgc ctgggcggtc ggcaatctcg
     1981 tctggaccct cgacctcgtc gtcctcgggc gtgcgccatc tggagccggc ctggccgacc
     2041 tcttctacgt ggcgttctat ccgctcgcgg tggccgcact cgtctgcctg cccggcgcgc
     2101 cgacgtccac ggtcggacgg ctgcgcaccg tcctcgacgg gatgatcgcc ggggcggcgc
     2161 tcgtcgtact gctctgggag ccgttgctcg cgccgctgct gcgccgggcg cagaccccgc
     2221 tcggtcacgc ggtcagcctg gcctacacgg tcggcgacgt ggccctgctc gccgtgctgg
     2281 tctcgctggc cgcgcactcg acgatgcgcg ggcgccgggc gggcgccctc ttgatcgccg
     2341 ggttcgccct cgtcacgatc gccgacaccg cctatgccgt cggggtctac caggacagct
     2401 acgtcagcgg cagcccggtc atcctcggct ggacgtttgg ctttgccctg ctgggcctgg
     2461 ccgccagcca gccggctggc accaccgacc cggcaaccag cgcggtgccg ccgcggcggt
     2521 ggcgggcgca cgtgccgctg ctgctggtcg cgccggccct gctgctggcg gcggtcacgg
     2581 tgtccggccg gggagcgctg tcgaccggga tggccctgcc cgggctgatc ggcgtggtgc
     2641 tcgtgctggg ccggcagacg ctggcggtgc gcgagaacgt cgagctcaac caccggctcg
     2701 agcacctggt tgcggccaag accgcgtcgc tgacccagct cgcctactcc gacccactga
     2761 ccggcctgcc caaccggcgt cggctgatcg aggagctggc cgcccccggc ccggccgacg
     2821 gctcgaccca gcgctggctc gggctcggcg acgtcgacgg cttcaagcag ctcaacgaca
     2881 cgctcggcca cctggccggt gacgatgccc tgcggcacat cgcgcaccag ctgcgcctgg
     2941 cccgcggggc cgacgacctc gtgttccggc tcggcggcga cgagttcgcc gtactcctgc
     3001 atgccgcctc cgcggaggcc gcgctgcaca ccgccaaccg gctcgcccag gcgctgcagg
     3061 aggaggcgct ggtggcgggc cggctgctgc ggctgcgcgc cagcctcggc atcgcgcccg
     3121 tcgtggccgg gcccgaccag gcgctgcggc acgccgacct ggcgatgtac gccgccaagc
     3181 aggccggcgg cggccaggtg aagctgttcg accacacgct gaccgacctg gccttcgcgc
     3241 ggatggacct ggagcacgac ctgcgggagg cggtggccac cggcgggctg ggcgtggtct
     3301 accagccgat cctcgatctg gcgaccggcg ccatcgtggg tgccgaggcg ctgtcgcgct
     3361 ggacgcaccc cgaccgcggg gtggtctccc ccgacgtgtt catcccgctc gccgaggagt
     3421 ccgacctcat cagcgtgctg ggcgccaacg tgctggccgc ggcgtgccga gccgcccacg
     3481 actggcgcac ccgtgacggt cggccgctgc cggtctgggt caacctctcg ccgcggcaga
     3541 tcgagcaccg gcacttcgtc gccgacgtgc tcgagctgct ggccacctcg cacctcgacc
     3601 cgcacgcgtt ggtgctcgag gtcaccgaag ggctgtttct cgacccggcc ggcaccgcgg
     3661 ccaccaccct gcagcagctg cgcggtgcgg gcgtgcggat cgccatcgac gacttcggca
     3721 ccggctacag ctcgctggcc cgcctgcacc agctgccggt ggacatcctc aagctcgacc
     3781 gggagttcgt cgaggcgctc agccgcgaca cgctggtcca gccggcgatc acccgggcca
     3841 tcatcgcgat ggccgcctcg ctcaacctga cgctggtcgc cgagggcgtc gagaccgagg
     3901 agcagcgggc cctgctgctg cgcgagggcg cccgcttcgg ccagggcttc gggctcggcc
     3961 gaccgcagtc cggcaccgcc ttcaccgcgc tcgcgccgct gaccctgcgc tagccgaaca
     4021 tccccccgac ctcgccggac gcggcgaccg catagccgaa gacggcgacc acgatgagcg
     4081 cgacgatgcc ggtgaccacc cagcgcagcc agggccgttt ggcgggggcg ggcaccagcg
     4141 acgtgtagtc gacccactcg cgcccgttga agacctgccg gccctgcagc ccgtccggct
     4201 cgtcggtggc acgcgccatc gatcagtccc ccgttccccg tcccgccgca tcccctcagc
     4261 ggccgcatct taagtgatca agcatggttc gcaacaggat caccccccac cgccgcgggg
     4321 acccgcagtt cttccgggac gcccgagcaa acgatcaagt caggcaccca ttcggccgat
     4381 aggtcggcgt gcgggatggg caagccgcgg cgatgcgcgc cgccgaggac gcggtgcagg
     4441 ccgccgaccc gtggctggcc gccgtgcggg ccgcggtcgc cgagccgtcg atccccgatc
     4501 tgcacgccca gcccatcgtc gacctggccc ggggcgaggt cgccggctac gaggtgctgg
     4561 cccgcttccc cggcccaccc caggccgccc ccgacgagtg gttcgcggtg gccgaccggg
     4621 cggggctggc cgcctacctg gaggcccgcg tcctcgaccg ggcgctgcag ctgcgctcga
     4681 gcctgccccg cggggccttc ctcagcgtca acgtgtccac ccacctgctg gccagcgagc
     4741 cggtgcagca ggtgctcgcc cgggctggct ccctggccgg gaccgtgctc gagctgaccg
     4801 agccggtgcc cgagcagcgc cctgacctga ccaacgccct ctgctcggtc accgcggtcg
     4861 gcggccggat cgccatcgac gacgcgggcg ccggccacca cggcatgcgc cagatggcca
     4921 ccgtgcgccc ggcgctggtc aagctcgacc ggtcgctggt cggcggcatc gacaccgacc
     4981 ccaccaaggc ggcggtggtg gagatgctcg gcgacttcgc caaccggctg gacgcccggc
     5041 tgctcgccga gggcatcgag accgaggccg agctgcggac cctcgtcggg ctgggcgtcc
     5101 cgctcggcca gggcttcctg ctggcccgac ccgacctccc ctgggtgggg ctgaccgagg
     5161 cggtcatcgc actcctgcgc agcgaggccc acgccggacg cgacgggcac gtcgccgagc
     5221 tggtcgagga cgccgacgtg gtgggggccg gctcgggcga ggcgccgcag accggccaga
     5281 tcatcgttga ggtcgaccgc ggcgagcacc cggtcgcgat gcgccacgcg accggcggtc
     5341 agctgcaccg cacgaccggg ccggccctgc tgatggtgct gccctccgac cggatcgagc
     5401 acgtggcgca cacggcgctg gcccgctcgt caccccaccg gttcgagccg ctggtctgcg
     5461 tcgacatcgc cggacggctg gtcggcgtcg tgcggatgga acggctggtc agcgcgctgg
     5521 ccgcgcgggc gagcgatccg ctcagcgcac ccggccatag ctgacgctgg tcgtccaggc
     5581 cgcgtgcttg ccgaccggct tgccggggcg gttggcctcc caccacatgt tggacccggc
     5641 gtagatcgcc acgtgactga ccccgctggc cttgtgcacg aacaccaggt cgcccgggcg
     5701 cagctggctg cgagtgatcc ggtcggtggc cgcccggatc tgggccgcgg tgcggggcag
     5761 gtcaatgccc agctgcttga acacgtagcc gacgtagccg gagcagtcga agcccgacgg
     5821 ggtggacccg ccccagacgt agggcacccc ggcgtagcgg gccgcgaccg cgaggacccg
     5881 ggcgccgaag ccggcgggca gcggggcggt ggcacgggtc gcggtgcgcg acgcgcgggc
     5941 cttggcctcg cgggcctcct tggcggcctt ggcggcggcg gccgccttgg ccgccttcgc
     6001 ctcggcggcc gccttcgcgg cggcggcctt gtcagcggcg gccttcgcgg cggcggcccg
     6061 gcggtccttg gccagctcga ccgcggcgat gatccggtct gcctcggcgg tgctggccgc
     6121 ctcgctggcg acgtaggcga ccacgtcggt gcgcgagagg tcgaccaccg ccggcgcgac
     6181 ggtgcgggtg gacgacccgc tcggcgcggc ggcggccagg ccgtcgacct ccggcggctc
     6241 agcctcggcg ccgataccgc tcgacaggag caggccgccg gagacggccg cgatcaacag
     6301 ggtcttggac gagcgcgcga gcgccgggga gcgcagcgcg ggtctgagca gggcggcagg
     6361 gtggacggcc ggccggagcg ccgaggggcg cagggcggcg acgggactgc ggcgtgggcg
     6421 gcggtgcttg gacacaacga tcctctccga cgcctgcggg gtgagctgtc gggctcgggc
     6481 tggagagtgc ccggccgccg tggcggcttc gccccaagga cgcgctggct gcgcgcccga
     6541 cgaacctggg tcccccgctc ctgccggacg ggttggtgca tcggggcccc acgtggggca
     6601 ggactcggcg tccgcgtggg gtctcgcacg gctgtgcgaa gtcccctaaa tgttacgaga
     6661 agatcacgga atggacaaat gccgtagatc aggcgacggt gatcgcgggc ccgtcgtcgg
     6721 cgccggccgc ggtgacaccg ggctcggggt cgaccccgtg ggcgctggcc tcgatgcgcg
     6781 ccagccggtc gcggaacacg tagcgcagca cgaccttgcc ggcggcggtc accggcagcg
     6841 cggccaccgc accgaccagt ccggtcaagg ttgccccgac gagcaccgcg acaaacacgg
     6901 ccagcgggct gagctcgacc gcgcgggaga acacccgcgg ggccaccacg tagttctcca
     6961 gctgctggta gacgacgaag tagacgacca ccgcgaccga ggtgacgatc cccagcggta
     7021 cggcgaacac gagcgcgacc agcgcgccga gcagcgcgcc gacctgcggg accgcgtcga
     7081 gaatggccac cgtgaccgcc agcagcgcgg cgtagggcag gtcgaggatg acgaagacga
     7141 tgtaggagca cacgccgtgc agcaggcaga tcgccagctg tccggtgatg tagccgccga
     7201 cccggccgag cgcgtcgctc atcacagcgg cccgctccgg gtcgcccagc gcggcggcgg
     7261 cgaaccggcg gatccgtggc agggccagca ggaagtagac cgacagcgcc gcaacggtga
     7321 acccggcgaa gatcaacccg atcacggccg tgacggcgcc gaacagcgcg ccgaccgagg
     7381 aggccagcag cgcgggcacc cggtcgagca gcgtctgggc ctgctcgcgg atgtcttcgc
     7441 gggccagcca gtctgagatc ggcccgctgc cctgcagccg ttcgcctagc tcctgcagct
     7501 ggccgggcag cgcggtgacc acctgggcga actgcccgat cgccggggcc agcgccagca
     7561 ccacgaacag cgcggtggcg agcagcccgg cgccgaagac cagggcgacc gccaagcccc
     7621 ggcgcagccc gcgccgcgcg accgccgcga ccagcgggtc gagcccggcc gcgatgagga
     7681 acccggacag caccaggatg agcacgccgc gggcccggta ggccagcagc gccacggcca
     7741 ccaccgcgag caccgtgacg gtgacccggg cgtagtgccc cagcgaggcc agctcgcggg
     7801 cggccgaccc cggcggcgcg ggcgaactcc cggccggctc ctccggcccg gacgactcgg
     7861 tcacggctct agcgccgtac gacgaagatg tgcgaggcga cctcgcggga gacctcggtc
     7921 tcctcgcccg cgctgcccag cacgaactgg cccggctgca gcgacggcgc ggcctgcacc
     7981 gcggcgccgg gccgcacccc ggctcggtcg agccggctca gcagcgtcga gtcggtctgc
     8041 accggctcgc ccatccgtcg tacgacgacc gtggtcgccg cgcccagctc cacctcgctg
     8101 agcgccacca ctccctcgag gaacgcatcg tgcgacggct tggcgcccag ctcgtcaagg
     8161 ccggggatcg ggttgccgta gggcgactcg gtcgggtggt cgagcagctc gagcagccgg
     8221 cgctcgaccg cctcgctgat cacgtgctcc cagcggcagg cctcggtgtg cacgtattcc
     8281 cagtcgagac cgatgacgtc gacgagcagc cgctcggcca gccggtgctt gcgcatcacc
     8341 cgggtggcca gcgactggcc ctcctcggtc agctcgagct ggcggtcacc gccgaccacg
     8401 agcagcccgt cacgctccat gcgggccacc gtctggctca cggtcgggtt gctctgcccg
     8461 agccgctcgg cgatccgggc ccgcagtggg acgacgccct cctcctgcag ctcgtagatc
     8521 gtgcgcaggt acatctccgt ggtgtcgatc aggtcgtggc tcacacgagt cattctcacc
     8581 catgagtggt ggcggcccgc tctctcgcga tgtagaggtg ggaagtggcg actcgggtgg
     8641 cgtcaagatt cgagggcgcg gcggcgtggg tgccgcaccg cggcgggttg ccgctgctgc
     8701 gcgcggccgc ccgtgactgc c
//
LOCUS       DATIBB010000130         6931 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_75367, whole genome shotgun sequence.
ACCESSION   DATIBB010000130 DATIBB010000000
VERSION     DATIBB010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 6931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6931
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75367"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..404)
                     /locus_tag="VLC50_04965"
     CDS             complement(<1..404)
                     /locus_tag="VLC50_04965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF26852.1"
                     /translation="MDGASGARWAGSGHPLLAGLHLTTTGLTTAAGPGRDRVSLLSDE
                     DTRAGLDAVAAAEGRLVAIRAALLAHAEVRRVREQLKARTTASWLADVARISGATARE
                     QVKVAGMLDGCPEAAGLLAAGAATLAHARIVA"
     gene            601..1347
                     /locus_tag="VLC50_04970"
     CDS             601..1347
                     /locus_tag="VLC50_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012014822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HSF26853.1"
                     /translation="MTTDIRVSTVLPARREDIELHTADGLTLVGELASPADREPLATL
                     ITLHPLPTHGGFMDSHVLRKASYRLPALAGIAVLRFNTRGTTSPRGTSEGQFDHARGE
                     RHDVAAAIEWATQRALPRRWLLGWSFGTDLALMYGADPRVEGAILLSPPLRHTQPEHL
                     AAWAASGQPVLALVPEHDDYLKPEPARERFAAIPQARVIGVERAKHLWVGEPYVRRVL
                     DEIVTQVAPQVPTPLPTVWDGPVETREMSA"
     gene            1344..2135
                     /locus_tag="VLC50_04975"
     CDS             1344..2135
                     /locus_tag="VLC50_04975"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HSF26854.1"
                     /translation="MTEPMAHLVDGPADAPVLLLLHGFPFAGDMWDDVRPLLGSQVRV
                     IVPDLLAGAHRSGEPSIDRLSDGVIGILDRLGVERAVVVGLSMGGYVALSVARDHSDR
                     VAGLGLLNTRSAPDTEEGRAGRVALAEQMTREESTRSLVETMPAALLGETTRRERPDV
                     VRRLEGLLAAPDPRDVAWGSLAMAARPDTTPVLAKVDVPTLVLTADEDTLIPPSFSEA
                     MAAALKNGSAPTTYVVVPGAGHMSAMERPEPVAAALRDLVSASEK"
     gene            complement(2142..3479)
                     /gene="ccrA"
                     /locus_tag="VLC50_04980"
     CDS             complement(2142..3479)
                     /gene="ccrA"
                     /locus_tag="VLC50_04980"
                     /EC_number="1.3.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007514748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043880 - crotonyl-CoA reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonyl-CoA carboxylase/reductase"
                     /protein_id="HSF26855.1"
                     /translation="MKDVLGAILAGDTPREAFADLAVPESYRAVTVHKDEVEMFAGRA
                     SRDKDPRESLHLDEVPTPELGPGEALVAVMASAINYNTVWTSIFEPMPTFSFLERLGR
                     TSPLGRRHDLPYHVVGSDLAGVVLRTGVGVHAWAPGDEVVAHCLSVELEHPAGHDDTM
                     LDPEQRIWGFETNFGGLAELALVKSNQLMPKPAHLSWEEAASPGLVNSTAYRQLVSKH
                     GAGMKLGDTVLIWGASGGLGSYATQIALASGATPVCVVSSPEKAAICRRMGAELIIDR
                     RAEDYRFWKDEHTPDPREWRRLGARIRELTSGRDVDIVFEHPGRETFGASVFVAKRGG
                     TIVTCASTSGFVHEYDNRYLWMNLKRIVGSHFANYREAWEANDLVARGLIHPTLSKSF
                     PLEATGQAAYEVHNNLHQGKVGVRCLAPEEGLGVRDTAMRERHLAAITRFRGA"
     gene            complement(3488..3910)
                     /gene="mce"
                     /locus_tag="VLC50_04985"
     CDS             complement(3488..3910)
                     /gene="mce"
                     /locus_tag="VLC50_04985"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012950102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HSF26856.1"
                     /translation="MFTAIDHVGIAVADLDAAIAWYRDNFDMHVVHEEVNEEQGVREA
                     MLQVGESDSCVQLLAPLTADSTIAAFLDRRGPGIQQMAYRVQDIEAVCATLQERGVRL
                     LYDSPRRGTAGSRVNFVHPKDAGGVLVELVEPADGAHP"
     gene            4027..5211
                     /locus_tag="VLC50_04990"
     CDS             4027..5211
                     /locus_tag="VLC50_04990"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005248351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HSF26857.1"
                     /translation="MSGSVIVAGARTPMGRLLGSLSSFSATDLGGIAIKGALEKSGVA
                     PDQVDYVIMGQVLQAGAGQIPARQAAVKAGIPMTVPALTINKVCLSGLDAIALADQLI
                     RAGEFEVVVAGGMESMTQAPHLLPRSREGFKYGDVALVDHMAYDGLWDVFTTQAMGAL
                     TEACNAELGISREDQDEFSARSHQRAAAAWKNGVFDDEVVAVEIPQRKGDPVVFKDDE
                     GIRPDTTAESLARLRPAFSKDGTITAGSSSQISDGAAAVVVMSKAKAEELGLTWLAEI
                     GAHGVVAGPDSTLQSQPSRAIEKACAREGITPADLDLVEINEAFASVGVKSMRDLGLS
                     ADIVNVNGGAIALGHPIGMSGARLALHLALELKRRGGGVGAASLCGGGGQGDALIVRV
                     PA"
     gene            5208..6173
                     /gene="meaB"
                     /locus_tag="VLC50_04995"
     CDS             5208..6173
                     /gene="meaB"
                     /locus_tag="VLC50_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018383718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HSF26858.1"
                     /translation="MTVRDLDVDELVRAARSGRPRAVARLISLVEGASPLLREVMAAL
                     APHSGSAYVIGLTGAPGVGKSTSTSALVTELRRRDLRVGVLAIDPSSPFSGGALLGDR
                     VRMQEHALDPGVFIRSMASRGHLGGLAWATPQALRVLDAAGCDVILIETVGVGQSEVE
                     IAGLADTTIVLLAPGMGDAIQAAKAGILEVGDVYAVNKADRDGADATVRELRHMISLG
                     ERRKPGDWRPPVVRMVAVKGEGLDELLEALDKHRGWLEQTGELHRRRHRRITEEIEAI
                     AVATLRSRIGDLRDGDLLDELAEAVLDGRADPYGAADRLVEGVTA"
     gene            6201..>6931
                     /locus_tag="VLC50_05000"
     CDS             6201..>6931
                     /locus_tag="VLC50_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005458491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSF26859.1"
                     /translation="MAATAGEPRTTVEVVDLRKSYDGVPAVDGVSFTVAEGEFFGILG
                     PNGAGKTTTLEMIEGIREPDGGQIRVLGEPPWPRNPRLLPRIGVQLQASAFFERLTAR
                     EQIETFGALYGVQSADAAQTLEMVGMTDRADTREDKLSGGQRQRLSIACALVHDPDVV
                     FLDEPTAALDPQARRNLWDVLRMIQAQGKTIIYTTHYIDEAEVLCDRVAILDHGRIID
                     IDTPAELVRRHCPERTVIVETEDSTA"
BASE COUNT          985 a         2487 c         2478 g          981 t
ORIGIN      
        1 tcggcaacga tccgggcgtg ggccagcgtg gcggccccgg ccgccagcag ccccgcggct
       61 tccgggcagc cgtcgagcat cccggcgacc ttgacctgct cacgggccgt cgccccgctg
      121 atccgggcca cgtccgccaa ccacgacgcc gtcgtacggg ccttcagctg ctcacgcacc
      181 cgacggacct cggcgtgggc caacagcgcg gcgcggatcg cgaccagccg gccctcggct
      241 gcggctaccg cgtccaaccc ggcgcgggtg tcctcatcgg agagcaggct gacccggtcc
      301 cggccgggcc cggccgcggt ggtcagcccg gtggtggtca ggtgcaggcc agccagcagc
      361 ggatgcccac tgcccgccca tctcgcgccg ctcgccccgt ccatgacccc acgctagagg
      421 ccaccactga cagtcccgcc cggcccgaaa cccgttgtgg acaaacggct tccgggtcgc
      481 gtctgtggat tctcccgacc gccattcgcg catgatcagt gcaggaaagg ggcgatggtt
      541 tgcgcatgat cagtgcgggg ttgggcgggg tggctcgggg cgggagctag ggtcggggcg
      601 atgactaccg acattcgcgt cagcacggtg cttcccgccc gacgggagga catcgagctg
      661 cacaccgccg acggattgac cctcgtcggc gagctggcca gccccgccga ccgagagccg
      721 ctcgccaccc tgatcacctt gcacccgctg cccacccatg gcgggttcat ggactcgcac
      781 gtgctgcgca aggcgtccta tcgactgccc gcgctggccg gcatcgccgt actccggttc
      841 aacacccggg gtacgacgag tccgcgcggc accagcgagg ggcagttcga ccacgcgcgc
      901 ggcgagcgcc acgacgtcgc cgcggccatc gagtgggcca cccagcgcgc gctgccgcgc
      961 cgttggctgc tcggctggtc gttcggcacc gacctggcac tgatgtacgg cgccgacccc
     1021 cgtgtcgagg gcgcgatcct gctctctccg ccgctcaggc acacccagcc cgagcacctc
     1081 gcggcctggg cggcctccgg ccaaccggtg ctggcgctgg tgcccgagca cgacgactac
     1141 ctcaagcccg agccggcccg ggagcgcttc gccgcgatcc cgcaggcgcg ggtcatcggt
     1201 gtcgagcgtg ccaagcacct ctgggtgggc gagccctacg tgcggcgagt tctcgacgag
     1261 atcgtcaccc aggtggcccc gcaggtgccg acgccgcttc cgacggtctg ggacgggccg
     1321 gtcgagacac gggagatgag tgcatgaccg agccgatggc tcacctggtc gacgggcccg
     1381 ccgatgcgcc ggtcctcctg ctgctgcacg ggtttccgtt cgccggcgac atgtgggacg
     1441 acgtacgccc gctgctcggg tcgcaggttc gcgtgatcgt ccccgacctg ctggctgggg
     1501 cgcaccgcag cggggagccg tcgatcgacc ggttgagtga cggtgtcatc ggcattctcg
     1561 accgcctcgg cgtcgagcgg gccgttgtcg tcgggctgtc gatgggcggc tacgtcgcct
     1621 tgtcggtggc ccgcgaccac agcgaccgcg tggccgggct cggcctgctc aacacccggt
     1681 cggcgcccga caccgaggag ggccgagcgg gtcgcgtcgc gctggccgag cagatgacgc
     1741 gtgaggagtc gacgcgttct ttggtcgaga ccatgccagc ggcactgctc ggtgagacca
     1801 ctcggcgcga gcgccctgac gtcgtacggc ggctggaggg cctgctcgcg gcgcccgacc
     1861 cgcgcgacgt ggcctggggc tcactcgcca tggcggctcg ccccgacacg acgccggtgc
     1921 tcgccaaggt cgacgtaccg accctggtcc tgaccgctga cgaggacacc ctgatcccac
     1981 cgagcttctc cgaggcgatg gccgccgcgc tcaagaacgg cagcgctcct acgacgtacg
     2041 tcgtggtgcc gggcgccggt cacatgagtg caatggagcg ccccgagccg gtcgccgccg
     2101 cgctgcgcga cctggtgtcg gcgagcgaga agtaaggccc gtcaggcgcc gcggaagcga
     2161 gtgatcgccg cgaggtggcg ctcgcgcatc gcggtgtcgc gcacccccag gccttcctcg
     2221 ggtgccaggc accgcacccc gaccttgccc tggtgcaggt tgttgtgcac ctcgtaggcg
     2281 gcctgcccgg tcgcctcgag cgggaacgac ttggacagcg tggggtggat cagcccgcgg
     2341 gccaccaggt cgttggcctc ccaggcctcg cggtagttgg cgaagtgcga tccgacgatg
     2401 cgcttgaggt tcatccagag atagcggttg tcgtattcat ggacgaagcc tgaggtcgac
     2461 gcacaggtga cgatcgtgcc gccgcgcttg gccacgaaca cgctggcgcc gaacgtctcc
     2521 cggccggggt gctcgaagac gatgtcgacg tcgcgcccgc tggtgagctc gcggatgcgg
     2581 gcaccgagcc gacgccactc cctggggtcg ggggtgtgct cgtccttcca gaaccggtag
     2641 tcctcggccc gccggtcgat gatcagctcg gcgcccatcc ggcggcagat cgcggccttc
     2701 tcgggcgagg agaccacgca caccggggtg gcaccggagg ccagcgcgat ctgggtggca
     2761 taggagccca gaccgccgga ggcgccccag atcagcacgg tgtcgccgag cttcatgccc
     2821 gcgccgtgct tggacaccag ctggcggtag gccgtcgagt tgaccagccc gggcgaggcg
     2881 gcctcctccc agctcaggtg cgccggcttg ggcatcagct gattggactt gaccagggcc
     2941 agctcggcca gcccgccgaa gttggtctcg aagccccaga tccgctgctc ggggtcgagc
     3001 atcgtgtcgt cgtggcctgc cgggtgctcg agctcgaccg acaggcagtg cgcgaccacc
     3061 tcgtcgccgg gtgcccaggc gtgcacgccg acgccggtgc gcagcaccac gccggccagg
     3121 tcggagccga ccacgtggta gggcaggtcg tggcggcggc ccagcggcga ggtgcggccc
     3181 agccgctcga ggaagctgaa cgtcggcatc ggctcgaaga tcgacgtcca cacggtgttg
     3241 tagttgatcg ccgaggccat caccgcgacc agcgcctcgc cggggccgag ctcgggcgtg
     3301 ggcacctcgt caaggtgcag cgactcgcgc gggtccttgt cgcgactggc ccggccggcg
     3361 aacatctcga cctcgtcctt gtggacggtg acggcccgat aggactcggg tacggcgagg
     3421 tcggcgaacg cctcccgtgg cgtgtcgccg gccaggatcg ccccgagcac gtccttcatc
     3481 gggtcggtca tggatgcgcg ccgtctgccg gctcgaccag ctcgaccagc acgccgccgg
     3541 catccttggg gtgcacgaag ttgacccgcg acccggcggt cccgcggcgc ggcgagtcgt
     3601 agagcagtct cacgccgcgc tcctgcagcg tcgcgcacac ggcctcgatg tcctgcacgc
     3661 ggtaggccat ctgctggatg cccggccccc ggcggtcgag gaatgccgcg atcgtcgagt
     3721 cggccgtcag cggcgcgagc agctgcacgc acgagtcgga ctcgccgacc tgcagcatgg
     3781 cctcgcgcac gccttgctcc tcgttgacct cctcgtgcac cacgtgcatg tcgaagttgt
     3841 cgcggtacca ggcgatcgcg gcgtcgaggt cggcgacggc gatgcctaca tgatcgatgg
     3901 cagtgaacac cgtctcactg tagggcgggt gcaatggggc ggcagtagtg ttggccacag
     3961 gctggaccag cgcccacgag gagcaccagc ccggtcatgg cgaagatcac acgaggaggc
     4021 acgaccgtgt ccggttccgt catcgtcgct ggagcccgca ccccgatggg ccggctgctc
     4081 ggctcgctgt cgagcttctc ggccaccgac ctcggcggca tcgcgatcaa gggggcgctg
     4141 gagaagtcgg gagtcgcccc cgaccaggtc gactacgtga tcatgggcca ggtcctgcag
     4201 gccggcgccg gccagatccc cgcccggcag gccgcggtca aggccggcat cccgatgacc
     4261 gtgcccgccc tgacgatcaa caaggtctgc ctgtccgggc tcgacgcgat cgcgctggca
     4321 gaccagctga tccgggccgg cgagttcgag gtcgtcgtcg ccggcggcat ggagtcgatg
     4381 acccaggcgc cccacctgct gccgcgctcc cgcgagggtt tcaagtacgg cgacgtcgcg
     4441 ctggtcgacc acatggccta cgacgggctg tgggacgtct tcaccaccca ggcgatgggg
     4501 gcgctgaccg aggcctgcaa cgccgagctg ggcatcagcc gcgaggacca ggacgagttc
     4561 tcggcccgct cccaccagcg cgcggcggcc gcgtggaaga acggcgtgtt cgacgacgag
     4621 gtcgtcgccg tcgagatccc acagcgcaag ggcgacccgg tggtgttcaa ggacgacgag
     4681 ggcatccgcc ccgacaccac ggccgagtcg ctggcgcggc tgcgcccggc attcagcaag
     4741 gacggcacga tcaccgccgg ctcgtcgtcg cagatctccg acggtgccgc cgcggtggtg
     4801 gtcatgagca aggccaaggc cgaggagctc gggctgacct ggctggccga gatcggcgcc
     4861 cacggcgtgg tggccgggcc cgactccacg ctgcagtccc agccctcgcg cgcgatcgag
     4921 aaggcctgcg ctcgtgaggg catcaccccg gccgacctcg acctcgtcga gatcaacgag
     4981 gcgttcgcca gcgtgggcgt caagtcgatg cgtgacctcg ggctgtcggc cgacatcgtc
     5041 aacgtcaacg gcggcgcgat cgcgctcggc cacccgatcg gcatgtcggg tgcccggctc
     5101 gcgctgcacc tggcgctcga gctcaagcgc cgcggtggcg gcgtcggcgc agcgtcgctg
     5161 tgcggcggcg gcggtcaggg cgacgccctg atcgtgcggg tgccggcctg acggtgcgcg
     5221 acctcgacgt cgacgagctg gtgcgggcgg cccggtcggg ccgcccgcgc gccgtagccc
     5281 ggctgatctc cctcgtggag ggcgcctcgc cgctgctgcg cgaggtcatg gcggcgctgg
     5341 ccccgcacag cgggagcgcc tacgtcatcg ggctcaccgg cgcccccggc gtcggcaagt
     5401 ccacctccac ctcggcgctg gtgaccgagc tgcgccggcg cgacctgcgg gtcggcgtgc
     5461 tggcgatcga cccctcctcg ccgttctccg gcggagcgct gctcggtgac cgggtgcgca
     5521 tgcaggagca cgcgctcgac cccggcgtct tcatccgctc gatggccagc cgcgggcacc
     5581 tgggcgggct ggcctgggcg actccgcagg ccctgcgggt gctcgacgcc gccggctgcg
     5641 acgtgatcct catcgagacc gtcggggtgg gccagtcaga ggtggagatc gcggggctgg
     5701 ccgacaccac gatcgtgctg ctcgcgcccg gcatgggcga cgcgatccag gccgccaagg
     5761 ccggcatcct cgaggtgggt gacgtctatg cggtcaataa ggccgaccgc gacggtgccg
     5821 acgcgacggt ccgcgagctg cgccacatga tctcgttggg cgagcgccgc aagccgggcg
     5881 actggcgccc gcccgtggtg cgcatggtcg ccgtgaaggg cgagggtctc gacgagctcc
     5941 tcgaggccct cgacaagcac cgcggctggc tcgagcagac cggtgagctg caccggcgcc
     6001 ggcaccggcg catcaccgag gagatcgagg cgatcgccgt ggccaccctg cgctcgcgca
     6061 tcggcgacct gcgtgacggc gacctgctcg acgagctcgc cgaggccgtc ctcgacgggc
     6121 gcgcagaccc ttacggcgcg gccgaccggc tcgtcgaagg ggtcacggcc tagccggtcc
     6181 ggcgactaac gtcgcccttc gtggcagcga ctgcggggga gccgcgcacc accgtcgagg
     6241 tggtcgacct gcgcaagtcc tacgacgggg tgccggccgt cgacggggtg tcgttcaccg
     6301 tggccgaggg ggagttcttc ggcatcctcg ggcccaacgg ggccggcaag accaccacgc
     6361 tggagatgat cgagggcatc cgcgagcccg acggtggaca gatcagggtc ctcggcgagc
     6421 cgccctggcc acgcaaccca cgcctgctgc cccggatcgg cgtgcagctg caggcgagcg
     6481 cattcttcga aaggctcacc gcgcgcgagc agatcgagac attcggtgcg ttgtacggcg
     6541 tccagtccgc cgacgcggcc cagacgctgg agatggtcgg catgaccgac cgggccgaca
     6601 cccgcgagga caagctctca ggcggccagc gccagcggct gtcgatcgcc tgcgccctcg
     6661 tgcacgaccc cgacgtcgtc ttcctcgacg agcccaccgc ggcgctggac ccgcaggcgc
     6721 gacgcaacct gtgggacgtg ctgcggatga tccaggccca aggcaagacg atcatctaca
     6781 ccacgcacta catcgacgag gccgaggtgc tctgcgaccg ggtcgcgatc ctcgaccacg
     6841 gccggatcat cgacatcgac accccggccg agctcgtgcg gcggcattgc cccgagcgaa
     6901 ccgtcatcgt cgagacggag gactcgacag c
//
LOCUS       DATIBB010000131         5704 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_1128197, whole genome shotgun sequence.
ACCESSION   DATIBB010000131 DATIBB010000000
VERSION     DATIBB010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 5704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5704
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1128197"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1502)
                     /locus_tag="VLC50_05005"
     CDS             complement(<1..1502)
                     /locus_tag="VLC50_05005"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007078014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HSF26860.1"
                     /translation="MTETTHGAAPTGDSAGTGRARRAAKGKGLSIGRIYTTAGVHPYD
                     EVTWERRDVVQTNWRSGEVIFEQRGVEFPDFWSLNSSMIVTTKYFRGAVGTPEREWSL
                     KQLIDRVVLTYVKAGKEFGYFATDADAEVFEHELTWALVHQVFSFNSPVWFNVGTSSP
                     QQVSACFILSVDDTMDSILNWYREEGLIFKGGSGAGLNLSRIRSSKELLSSGGTASGP
                     VSFMRGADASAGTIKSGGATRRAAKMVVLDVDHPDIEEFVQTKAREEDKIRVLRDAGF
                     DMDLGGKDITSVQYQNANNSVRVSDEFMRAVEQGAAFGLRARATGEVIETVDARDLWT
                     KIATAAWECADPGVQYDDTINDWHTNPETGRITASNPCSEYLSLDNSSCNLASLNLLK
                     FLKDDDTFDTTTFVKMVELVITAMDISICFADFPTESIGVTTRDYRQLGIGYANLGAL
                     LMASGLAYDSDGGRALAAAITSLMTGTAYKRSAELAGIVGPYAGFARNADA"
     gene            complement(1643..2137)
                     /gene="nrdR"
                     /locus_tag="VLC50_05010"
     CDS             complement(1643..2137)
                     /gene="nrdR"
                     /locus_tag="VLC50_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HSF26861.1"
                     /translation="MHCPYCRHTDSRVVDSRVTDDGAAIRRRRQCPECARRFTTVETA
                     SLTVVKRSGASEPFSRAKVLVGVRKACQGRPVTEDALARLAQRVEETVRATGSAQVDA
                     YDVGLAILGPLRELDEVAYLRFASVYRSFESLSDFEAEIALLRAEHDPVGVEPPRRGG
                     QRDG"
     gene            complement(2388..3005)
                     /locus_tag="VLC50_05015"
     CDS             complement(2388..3005)
                     /locus_tag="VLC50_05015"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HSF26862.1"
                     /translation="MTDVVLIPSVLGVRPAIRQVQERLGAGHRVHVVEVYAPGLTFDD
                     YDPAIAHWRSLGLDDTPRLIHERVSDLPAEVVYAGFSAGAALALVLAATRPGARGALA
                     LAGGPPLAALGVDRWPAGVPVQAHAMVDDPFRDPDEFVGLQHDVTRAGAAFELFDYPG
                     GGHLFADEGLPEYDAEAAALMWSRVRTFLAKVGDSPDRSSGTVGQ"
     gene            complement(3015..3479)
                     /locus_tag="VLC50_05020"
     CDS             complement(3015..3479)
                     /locus_tag="VLC50_05020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF26863.1"
                     /translation="MMIEDPVAGPSGRGAVRVGQLLVDLGLVTTLEVARAVAAQRDGD
                     PRRLGEILVAQGACTTDTLEQMLRTHGVPSAPRRTVTVDEEGLRELISVRDALLQRAA
                     ESGDPELLLLAERLDRVVTDLAAGVPVRRVGARAVRSVVATPRRSGTVRGHR"
     gene            3650..4420
                     /locus_tag="VLC50_05025"
     CDS             3650..4420
                     /locus_tag="VLC50_05025"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020125953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HSF26864.1"
                     /translation="MAVDRSRLSHQVRYAMAHPDRIGPHARRLLRDAWLRVRHRGDHV
                     GYYRAVMRADVTTRNADAAVGGRSRDRWLALGQMQFDYLHGHGLRPEHRLLEIGCGNL
                     RAGRLFIDYLEPGHYYGLDISPDILVAATRTVVDYGLQDKLPHLTPVRDLRLDFLPSA
                     HFDVVHAHSVFSHSPLEVIDECLAHVGRVLRRDGFFDFTFDRTQGREHQVLREDFYYR
                     TETLVALAERHGLTARLMDDWETLPHGQSKIRVRHRDQ"
     gene            complement(4421..4816)
                     /locus_tag="VLC50_05030"
     CDS             complement(4421..4816)
                     /locus_tag="VLC50_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013583384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HSF26865.1"
                     /translation="MTTTMALRAPALRVRPATTAGAPLGPARRRLRLTRRGRAVRAVV
                     LVLALTLVFLVVSALGHSVVSASTSESGEGPALRALVVQPGQTLWQIAGQVAPDADPR
                     DTVVRLERLNGLDGALIQVGQRLLVPVNG"
     gene            5038..>5704
                     /gene="lexA"
                     /locus_tag="VLC50_05035"
     CDS             5038..>5704
                     /gene="lexA"
                     /locus_tag="VLC50_05035"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019137456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HSF26866.1"
                     /translation="MGTVREIPDGPADESGLTPRQRRVLEVIRDSVERRGYPPSMREI
                     GEAVGLTSPSSVAHQLASLERKGFVRRDPNRPRAIEVLGHEQSAGTADATPTPHDETG
                     ASDERPAPSYVPVVGRIAAGGPILAEQAVEDVFPLPRQIVGDGALFLLRVAGESMVDA
                     AICDGDWVVVRQQPVAEQGEIVAAMIDGEATVKVFSRKDGHIWLLPRNDDYAPIPGDD
                     AVVL"
BASE COUNT          818 a         1998 c         2072 g          816 t
ORIGIN      
        1 gcgtcggcgt tgcgggcgaa gccggcgtag ggcccgacga tgccggcgag ctcggccgag
       61 cgcttgtagg ccgtgcccgt catcagcgac gtgatcgcgg cggccagcgc ccggccaccg
      121 tcggagtcgt aggccagccc ggaggccatc agcagcgcgc ccaggttggc gtagccgatg
      181 ccgagctggc ggtagtcgcg ggtggtcacc ccgatcgact cggtcgggaa gtcggcgaag
      241 cagatcgaga tgtccatcgc ggtgatgacc agctcgacca tcttgacgaa cgtcgtcgtg
      301 tcgaacgtgt cgtcgtcctt gaggaacttc aacaggttga gcgacgcgag gttgcacgag
      361 gagttgtcga gcgagaggta ttccgagcac gggttgctcg cggtgatccg cccggtctcg
      421 gggttggtgt gccagtcgtt gatcgtgtcg tcgtactgca ccccggggtc ggcgcactcc
      481 caggccgccg tggcgatctt ggtccacagg tcgcgggcgt cgacggtctc gatcacctcg
      541 ccggtggcgc gggcccgcag cccgaaggcc gcgccctgct cgaccgcgcg catgaactcg
      601 tcgctgaccc gcaccgagtt gttggcgttc tggtactgca ccgaggtgat gtccttgccg
      661 cccaggtcca tgtcgaaccc ggcgtcgcgc aacacccgga tcttgtcttc ctcgcgggcc
      721 ttggtctgga cgaactcctc gatgtcgggg tggtcgacgt cgagcacgac catcttggcg
      781 gcccgccggg tggccccgcc ggacttgatg gtgccggccg acgcgtcggc gccgcgcatg
      841 aaggagaccg ggccggaggc ggtgccgccg gaggagagca gctccttgga cgagcggatc
      901 cgcgacaggt tgagcccggc gccggagccg cccttgaaga tcagcccctc ttcgcggtac
      961 cagttgagga tcgagtccat cgtgtcgtcg accgacagga tgaagcacgc gctgacctgc
     1021 tgcggcgagg acgtgccgac gttgaaccag accggggagt tgaacgagaa cacctggtgg
     1081 accagcgccc aggtcagctc gtgctcgaac acctcggcgt cggcgtcggt cgcgaagtag
     1141 ccgaactcct tgccggcctt gacgtaggtc agcacgaccc ggtcgatgag ctgcttgagg
     1201 ctccactcgc gctcaggcgt gccgaccgca ccgcggaagt acttggtggt gacgatcatc
     1261 gaggagttga gcgaccagaa gtcggggaac tcgaccccgc gctgctcgaa gatcacctcg
     1321 ccggagcgcc agttggtctg cacgacgtcg cggcgctccc aggtgacctc gtcgtagggg
     1381 tgcacgcccg cggtggtgta gatgcgcccg atcgacaggc ccttgccctt ggcggccctg
     1441 cgcgcgcgac ccgtgccggc gctgtccccg gtcggtgcgg cgccgtgcgt cgtctcggtc
     1501 atcgatatcc cctccgcggg cccggatggc ccattcagtg tcaacagttg tcgtgctgcc
     1561 gggatgcccc cggccaggtc gtgtgggtgg tgtcgtcgta cggcgtgctg gcttcggcgc
     1621 cgccgtgggg cggcaggtgt ggtcagccgt cgcgttggcc gccacggcga ggcggttcga
     1681 cgccgaccgg gtcgtgctcg gcccgcagca gcgcgatctc ggcctcgaag tcggagagcg
     1741 actcgaacga gcggtagacc gaggcgaacc gcaggtaggc gacctcgtcg agctcgcgca
     1801 acggcccgag gatggccagg ccgacgtcgt aggcatccac ctgggccgac ccggtggccc
     1861 gcacggtctc ctcgacccgc tgggccagtc gggccagcgc gtcctcggtg accgggcgtc
     1921 cctggcaggc cttgcggacg ccgacgagga ccttggcccg gctgaacggc tcgctcgccc
     1981 cggaccgctt gaccacggtg aggctggcgg tctcgacggt ggtgaagcga cgggcgcact
     2041 cggggcactg gcggcggcgc cggatcgcgg cgccgtcgtc ggtcacccgg ctgtcgacga
     2101 cccggctgtc ggtgtgccgg cagtagggac agtgcatgcc ggcctcctct gctgtgacgg
     2161 tctctgcggc gctgggacag gccctggtca ggccccgcct gtggacaaag ctgtgggcat
     2221 ctcggtcggt cgtgtgggta agtcgcctcg ggctgtgaca caacctgtgg atggcttaca
     2281 ccagtgtaac tactagatgt ggtggtgacc gtagaactct gccgatgtga tcgcaagcac
     2341 ttcgcggcca tcggggcgtg tcggtgcggg cggtctcgcg accgccctca ctggcctacg
     2401 gtgccactgc tccgatcggg tgaatcgccg accttggcga ggaaggtccg cacccgcgac
     2461 cacatcagcg cggccgcctc ggcgtcgtac tcgggcagcc cctcatcggc gaagaggtga
     2521 cccccacccg ggtagtcgaa cagctcgaag gcggcaccgg cgcgggtgac gtcgtgctgc
     2581 aggcccacga actcgtcggg gtcgcggaac gggtcgtcga ccatggcgtg ggcctgcacc
     2641 gggacgccgg ccggccagcg gtccacgccc agcgcggcca gcggcgggcc gccggcgagt
     2701 gccagcgccc cccgcgcgcc cggccgggtc gcggcgagca cgagcgcgag cgccgccccg
     2761 gccgagaagc cggcatagac gacctcggct ggcaggtcgg acacccgctc atggatcagc
     2821 ctcggcgtgt cgtcgaggcc gagcgagcgc cagtgggcga tggcggggtc gtagtcgtcg
     2881 aaggtgagcc caggggcata gacctcgacg acgtggactc ggtggccggc gccgaggcgc
     2941 tcttgcacct ggcggatcgc ggggcgcacc ccgagcacgg agggaatcag cacgacatca
     3001 gtcatgcgcc cttcctaacg atgacctctg acagtcccgg atcgacgggg ggtcgccacg
     3061 accgaacgca ccgcccgcgc gccgacccgc cgcacgggca caccggccgc caggtcggtg
     3121 accacccggt cgaggcgctc ggcgagcagc agcagctcgg ggtcaccgct ctcggcggcc
     3181 cgctggagca gcgcgtcgcg cacgctgatc agctcgcgca gcccctcctc gtcgacggtc
     3241 acggtgcgcc gcggcgccga cgggacgccg tgggtgcgca gcatctgctc gagcgtgtcg
     3301 gtggtgcagg cgccctgggc caccaggatc tcgccgagcc gtcgggggtc gccgtcgcgc
     3361 tgggccgcca cggcacgggc cacctcgagg gtcgtgacca gaccgaggtc gacgagcagc
     3421 tggcccactc gcaccgcacc gcgtccactg ggaccggcca ccggatcctc gatcatcacg
     3481 aagcgatggt ggcgcgtgcg ggagtcacgg tcaatgatcg gcccgtcgtg tctgtggccg
     3541 gcgggctcac ggtccaccgc ccaggcggcg cccgctgcgg gcgatcatcg gccacgatgg
     3601 gcagcgacga ttggccggcg aggtcagcac gagcgggagg ggggtagaca tggcggtcga
     3661 caggtcacgg ctgagccacc aggtgcgtta tgcgatggcg cacccggatc ggatcggccc
     3721 acacgcccgc cggctgctgc gcgacgcgtg gctgcgggtc aggcaccgcg gcgaccacgt
     3781 gggctactac cgcgcggtga tgcgcgccga cgtgaccacc cgcaacgctg acgccgccgt
     3841 cggcgggcgc agcagagatc gctggctggc cctggggcag atgcagttcg actacctgca
     3901 cgggcacggc ctgcgcccag agcaccggct gctcgagatc gggtgcggca acctgcgcgc
     3961 cggccggctg ttcatcgact acctcgagcc cggccactac tacgggctcg acatctcccc
     4021 cgacatcctc gtcgcggcca cccgcacggt cgtggactac gggctgcagg acaagctgcc
     4081 ccacctcacc ccggtgcgcg acctgcgcct ggacttcctg ccgagcgccc acttcgacgt
     4141 ggtgcacgcc cacagcgtct tctcgcactc gccgctcgag gtgatcgacg agtgcctggc
     4201 ccacgtgggc cgggtgctgc gccgggacgg gttcttcgac ttcacgttcg accgcacgca
     4261 gggccgcgag catcaggtgc tgcgggagga cttctactac cgcaccgaga cgctggtcgc
     4321 gctggccgag cggcacgggc tgacggcgcg gctgatggac gactgggaga cgctgccgca
     4381 cggccagtcg aagatccgcg tcagacaccg cgaccagtga tcagccgttg acgggcacga
     4441 gcagccgctg gccgacctga atcagggcgc cgtcgagccc gttgagccgc tcgaggcgga
     4501 ccacggtgtc gcgcgggtcg gcgtcggggg ccacctggcc ggcgatctgc cacagcgtct
     4561 ggcccggctg caccaccagc gcccgcagcg ccgggccctc gccgctctcg ctggtgctgg
     4621 ctgagaccac cgagtgcccg agcgccgaga cgactaggaa gacgagcgtc agcgcgagca
     4681 cgagcaccac cgcccgcacg gcccgccccc gcctggtcag ccgcagccgg cgccgcgccg
     4741 ggccaagcgg cgcgcccgcc gtcgtggccg ggcggacgcg cagggccggt gcccgcaacg
     4801 ccatcgtcgt ggtcatcgtc gtcatcgtgg cctccgcgct ggtccagccg aacgggtgtt
     4861 cgatcgaacg tctgtgcgac gttatagcgc acccgaccga cggcgtccac cgacacgtcg
     4921 tacacgtgtt tgaaacgcgt gttcgaggtc gctacggtga gcagtgcagc agagggcacc
     4981 gacagtcctc ggccggcatc cgccagccga ggtcacggca cgagcggagg cggcaccgtg
     5041 ggcaccgtgc gcgagattcc cgacggcccg gccgacgaga gcggcctgac cccccgccag
     5101 cgccgagtgc tggaggtgat ccgcgactcg gtcgagcggc gcggctaccc gccgagcatg
     5161 cgcgagatcg gggaagcggt cgggctgacc tccccctcgt cggtcgccca ccagctggcc
     5221 tccctcgagc gcaaggggtt cgtccgccgc gaccccaacc gaccccgggc catcgaggtg
     5281 ctcgggcacg agcaaagcgc cggcactgct gatgccacgc ccacccctca cgacgagacc
     5341 ggcgccagcg acgagcggcc ggcgccgtcc tacgtgcccg tcgtcgggcg catcgcggcc
     5401 ggcgggccca tcctggccga gcaggcggtc gaggacgtct tcccgctgcc ccgccagatc
     5461 gtgggcgacg gcgcgctgtt cctgctgcgg gtggccggcg agtcgatggt cgacgccgcg
     5521 atctgtgacg gcgactgggt ggtggtccgc cagcagccgg tggccgagca gggtgagatc
     5581 gtcgccgcga tgatcgacgg agaggcgacc gtcaaggtgt tctcccgcaa ggacgggcac
     5641 atctggctgc tgccgcgcaa cgacgactac gccccgatcc ccggcgacga cgccgtggtg
     5701 ctgg
//
LOCUS       DATIBB010000132         9728 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_733944, whole genome shotgun sequence.
ACCESSION   DATIBB010000132 DATIBB010000000
VERSION     DATIBB010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 9728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9728
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_733944"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1799)
                     /locus_tag="VLC50_05040"
     CDS             complement(<1..1799)
                     /locus_tag="VLC50_05040"
                     /inference="COORDINATES: protein motif:HMM:NF014741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HSF26867.1"
                     /translation="MFDNEASDAGSGSGALVPRAGRARSWSEGTAWTQTMSLTAGQRQ
                     VLDRVLTKAAPVVCEGVVLPEWAGWSAGPALGAVLAGLDLGSLDEAGLVEALAAFDRQ
                     GSWCRAGQLAAITALAGRRAPAEPVSAPDGSGADWADRGVAHHRDVSEFLVQEVAARL
                     AVSKFDAGARVGDALDLLGPGGCQTTFAALVCGVVDPAKAHVVAETVRGLDLGGEDVE
                     DVPGLLEARLLPGTVTRTVRQARRQGQRLLLELDPGAAQERAEAARRRRRVILEPMPD
                     STAWLSTLLPAEDAVAVYTCLDLLARHRHAPGDPRGIDARRADALVQLITGHPLISGR
                     CWCTGTCGGTHTSTCTGAGTTPGAGATCGSGAGAGCAAVPTDASRTPVGGIGASGRRK
                     ALVHLSVPALTLLGESVAPAWLHGYGYLPYGVAAAIAADATWRRLLTDPFTGTVIGAE
                     VTQHDPPASMVELVNARYRECGFGPCTREAEACDLDHAIPYPDGPTSPSNLTPECRPE
                     HRMCTLATGWRLARDDGGFVWISPTGHRYPIEPAQVGTVLAAIANGTYQVTPDEPPAE
                     PGPTVDGPPVDGPPVDGQPVDGPSLDDLPWDVLR"
     gene            complement(1853..2685)
                     /locus_tag="VLC50_05045"
                     /pseudo
     CDS             complement(1853..2685)
                     /locus_tag="VLC50_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020577225.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
     gene            complement(2736..2948)
                     /locus_tag="VLC50_05050"
     CDS             complement(2736..2948)
                     /locus_tag="VLC50_05050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF26868.1"
                     /translation="MCSVFETMLIIGGLFVFAVFIARVGFSKQQFENARKRSFLSLYV
                     LYPLFLWVGPPLAVVGGVGTLVICVL"
     gene            complement(3157..3948)
                     /locus_tag="VLC50_05055"
     CDS             complement(3157..3948)
                     /locus_tag="VLC50_05055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HSF26869.1"
                     /translation="MSVPVTSAAAPSRRTTLWRVELLRLWRTRRWVALLAVYLLFGLL
                     GPLSARYIREILARAGGDITIVAPDPVPADGLAAFVNNASQIGLVVVVIVAALALTLD
                     ARPGLAVFYRTRVRRPADLVVPRFVVAASAAALAYLAGLLAAVYETVVLLGGIDPLDV
                     AMGGLLGMLYLVFAVAVVALAASRSRSVLATVGITLGALLALPIVGVWAPLGRWLPSA
                     LVGAQDALARGVKTPGDYLGATAVTLVLTVGLVVLACRLIGRRDL"
     gene            complement(3945..4871)
                     /locus_tag="VLC50_05060"
     CDS             complement(3945..4871)
                     /locus_tag="VLC50_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017581361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSF26870.1"
                     /translation="MSAPVVATTGLTRDYGQGAGIFDVDLAVPAGSVYGLVGPNGAGK
                     TTLLSIVTGLRRADRGEVRLGVDRRRVAVCADVPEFEPWLTATEVVELAAGLVRDADG
                     ASTGPSDRRAVAEALGRAGLTEAADRRVGGFSRGMTQRLGLAAALVGDPEVLVLDEPT
                     SALDPAGRAEVLDLVVGMRGRTTVIFSSHILGDVQRVCDTVGVLRAGRLLYQGGLDRL
                     LAEHVRPRWAVRLRSGSERLVAALGAEPWVVQAEPADGQTVHVEARTLAEGEHGVVAT
                     AAALGLAVVSLTPLEADLESAFLSLTHAAVAR"
     gene            complement(4868..5068)
                     /locus_tag="VLC50_05065"
     CDS             complement(4868..5068)
                     /locus_tag="VLC50_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019926299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF26871.1"
                     /translation="MSSAGISWAALAPLILLAVAWVAFCLVQIVRAPGVRGLPRWAWV
                     LVVVLSVPLGGVVFLLAGRAER"
     gene            complement(5132..6424)
                     /locus_tag="VLC50_05070"
     CDS             complement(5132..6424)
                     /locus_tag="VLC50_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013227556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsB/NhaD family transporter"
                     /protein_id="HSF26872.1"
                     /translation="MSAPLAIAVFGAAYVLIASEKVNRVAAAVGGALVVLALGVVGPN
                     DSFFSRETGIDWNVIFLLLGMMIIVGVVKQTGIFDYLAIWAAKRAKGDPFRVMVLLVL
                     ITAVASAALDNVTTVLLVAPVTLLVCDRLLLNPVPYLIAEAMASNIGGTATLIGDPPN
                     IIIAGRSGLSFNDFLVNLAPVVVIILVVFIGLCRVLFRKAFVAHPERVDEVMSLVERE
                     AITNPRLLVRSMVVLALVLAGFVLHSALHLEPSVVALTGAGLLVLLSGMEPAGYLADV
                     EWETLVFFMGLFIMVGALVKVGTIEKLATWATEATGGSVFVASMLLLVVSGVLSGIVD
                     NIPYVATMTPLVEQLVDAAGGLGAGAVLWWSLALGADLGGNATAIGASANVVVLGIAK
                     RNGTPISFWEFTRYGLVVATVSIAICVPYVWLRYFVLA"
     gene            complement(6421..6870)
                     /locus_tag="VLC50_05075"
     CDS             complement(6421..6870)
                     /locus_tag="VLC50_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020578060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HSF26873.1"
                     /translation="MLAREIAEDYPVVHADADVYEAAHAMAVRRLPGIIVVDGDPHRP
                     IAVLPGTQVVRLLIPHYVQDDPTLARVFTEDDAAELFERLRGRTVRSLLPDGARELPV
                     VRGDDQVLEIAALMARLHSPLIVVSEHDEILGAVTTSRLLEILLPDV"
     gene            7008..8681
                     /locus_tag="VLC50_05080"
     CDS             7008..8681
                     /locus_tag="VLC50_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014143177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair helicase XPB"
                     /protein_id="HSF26874.1"
                     /translation="MTDGPLIVQSDKTLLLEVDHPQAAEARREIAPFAELERSPEHVH
                     TYRVTPLGLWNARAAGHDAEQVVDALLRWSRYPVPHALLVDVAETMDRYGRLRLEKDP
                     AHGLVLISTDRAVLEEVLRSKKIAPLVGERVAEDTVVVHPSERGSLKQVLLKLGWPAE
                     DLAGYVDGEAHPIALREDGWALRPYQAEAAEGFWHGGSGVVVLPCGAGKTIVGAAAMA
                     RAQATTLILVTNTVAARQWRHELLRRTSLTEDEIGEYSGAKKEIRPVTIATYQVMTTR
                     RKGVYAHLELFDSRDWGLIVYDEVHLLPAPIFRFTADLQARRRLGLTATLVREDGREG
                     DVFSLIGPKRYDAPWKDIEAQGYIAPADCVEVRVTLSERERMLYATAEPEERYRLAAS
                     SDTKNRIVEALVAAHAGEPTLVIGQYIDQLDELGEHLGAPVIKGETTVRERERLFEAF
                     RTGELSTLVVSKVANFSIDLPEASVAIQVSGTFGSRQEEAQRLGRVLRPKADGRGARF
                     YAVVARDTLDQDYAAHRQRFLAEQGYAYRIIDSDDVLRGDLTGDGPAVD"
     gene            complement(8612..>9728)
                     /locus_tag="VLC50_05085"
     CDS             complement(8612..>9728)
                     /locus_tag="VLC50_05085"
                     /EC_number="3.4.13.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018841906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HSF26875.1"
                     /translation="TDWPRLRAGGVGGQFWSVYVPSTLPGSSAVTATLEQLDVVRRMC
                     ARWPGSFAWCTSSAEVSQAMAAGRLASLAGMEGGHSIDSSLAALRMMHALGARYMTLT
                     HNDNVPWADSATDSPRVGGLTRFGEEVVREMNRLGMLVDLSHVSPDTMRHALMVAAAP
                     VVFSHSSARAVCDHVRCVPDDVLASLPANGGVCMVTFVPAFVSEPCRAWDEKVRAEMV
                     RQGLPPGDWQARMRVAAEVDARSPRPRASAADVADHVCHVREVAGIDHVGLGGDYDGV
                     DVQPLGMEDVTGYPTLVAELLERGWTGDDVRRLTHGNVMRVLGSAEVVAARLQTERGP
                     SVSRIETLDGCGAIDQSTAGPSPVRSPRSTSSESMIR"
BASE COUNT         1625 a         3631 c         3276 g         1196 t
ORIGIN      
        1 cgcaggacgt cccagggcag gtcgtcaagg gatgggccgt cgacgggctg gccgtcgaca
       61 ggtgggccgt cgacaggtgg gccgtcgacc gtcgggccgg gctcggcggg cggttcgtcg
      121 ggcgtgacct ggtaggtgcc gttagcgatg gccgccagca cggtgccgac ctgggccggc
      181 tcgatcgggt accggtgccc ggtcggggag atccacacga accccccgtc gtcacgggcc
      241 aaccgccacc cggtcgccag ggtgcacatc cgatgctccg gccggcactc cggggtcaga
      301 ttcgacgggc tggtcggccc gtccgggtag gggatcgcgt ggtccaggtc gcacgcctcc
      361 gcttcccggg tgcacgggcc gaacccgcac tcccggtagc gagcattcac cagctcgacc
      421 atcgaggccg gcgggtcgtg ctgggtcacc tccgccccaa tcactgttcc ggtgaacggg
      481 tcggtcagca gccgccgcca cgtcgcgtcc gccgcgatcg cggcggccac cccgtagggc
      541 aggtagccgt agccgtgcag ccacgccggc gccacgctct ccccgagcag ggtcaacgcc
      601 ggcacgctca ggtgcaccaa cgccttccgc cgccccgacg cgccgatccc cccgaccggg
      661 gtccgcgacg cgtcggtggg caccgccgca cagcccgccc cggcgccgga cccgcaggtg
      721 gccccggcgc cgggagtcgt gccggccccg gtgcaggtgc tggtgtgggt gccgccgcag
      781 gtgccggtgc accagcaccg cccgctgatc aacggatgcc cggtgatcaa ctgcacgagc
      841 gcgtcggcgc gacgggcgtc gatgccacgc ggatcacccg gcgcgtgccg gtggcgggcc
      901 agcaggtcca ggcacgtgta aacggccacc gcgtcctcgg ccggcaacag ggtcgacagc
      961 cacgccgtgg aatccggcat cggctccaag atcacccgcc ggcgccggcg ggccgcctcg
     1021 gctcgttcct gcgccgcgcc cgggtccagc tcgagcagca gccgctggcc ctgccggcgc
     1081 gcctgacgca ccgtgcgggt gaccgtgccc ggcagcagcc gcgcctccaa cagcccgggc
     1141 acatcctcca cgtcctcacc gcccagatcc agcccgcgga ccgtctcggc caccacgtgc
     1201 gccttggctg ggtccaccac cccgcacacc agggccgcaa acgtcgtttg gcatccgccc
     1261 ggacccagca ggtccaacgc atcccccacc cgggcgccgg cgtcgaactt cgacaccgcc
     1321 aaccgggccg cgacctcctg caccaagaac tcgctgacgt cccgatgatg cgccacaccc
     1381 cgatcggccc agtccgcgcc cgacccgtca ggcgccgaca ctggctcggc cggtgcccgc
     1441 cgacccgcca acgcggtgat cgctgccagc tgccccgccc gacaccacga gccctgccgg
     1501 tcaaaggccg ccaacgcctc caccagcccc gcctcgtcca agctgcccag atccagcccc
     1561 gccaacaccg cccccaacgc cggacccgcc gaccagcccg cccactccgg caacaccacc
     1621 ccctcacaca ccaccggcgc tgccttcgtg agcacgcggt cgagcacctg ccgctgaccg
     1681 gcggtcaacg acatcgtctg cgtccacgcc gtgccctcgg accatgagcg agctcgaccc
     1741 gcccgcggca ccaacgcccc cgacccggac cccgcatccg acgcctcgtt atcgaacatg
     1801 tgtacgagtg tagtaggctt cgcttgcgat gtccagcatg tgggtgcgtt gattagagac
     1861 agttcccatg tggatcaacc agccacgcct gcaccgacgc cgtacgcctg cgtctttcgg
     1921 caaggaagtc ctcccaggtg cgccggccga cggcccggtt gggggcgagg gtggccccag
     1981 cccgcaccgc ggcggccgct cgacctggta gccgggccgg caacacggga cggcggcggt
     2041 ggatcgctct cagagagtcg gtcacgagct cggtgatgcc gtagatgcgt ggtccgcaat
     2101 gtcgggtacg agccccgccg gcttgtcgag tgcaagctcc accagccggg cgcccacctc
     2161 acccacgtca acaggctgga aacgggatcc cgcgggcacg ggtaccagca gcatcgcaag
     2221 ggcctgcacc accgtgagga tgaggtcgtg aaactgggtg gcgcgcaggg tggtccacgg
     2281 cagcccggag ctggcaaccg ccatttcggc agcccgcttg gacgcgaaat agccaaacat
     2341 cgcgcggtcc acacggctga cgaccgggat gcggtcggcg cccacgaccg aaatgcaggc
     2401 gaggtggttg acgccggaat gcctcgctgc ctgcaccagg ttgcgggcct tgacctcgtc
     2461 gcctttggag ctgccggcca ggtgcacgat cgtgcctgct ccgcgcacgg ccgcttcgac
     2521 gccggccccc gtggccagat cagccgcgac gagctcgacg ctgtggtcgg tcgccttctc
     2581 gtgccggctc aggacccgaa cgtcatgcct cgcgtcgcgc agcagcgcca cgacctggcg
     2641 ccccaacgtg cccgtgcctc cggtgacaag gatgggttca gccatgacaa ccatgctgag
     2701 cccaaccacg cccgcgtgcg ccaagttcta gccctctaaa ggacacagat gaccaaggtt
     2761 ccgacaccgc cgaccacggc aagcggcggc ccgacccaga gaaacaacgg gtagaggacg
     2821 taaaggctca gaaaagatcg ctttcgagcg ttctcgaatt gttgcttgct gaagccaacg
     2881 cgagcaatga atacagcgaa gacaaacagg cccccgatga tcagcatggt ttcaaagacc
     2941 gagcacatgg tctccctcat aagcgagagc tgagcctgtg taggagcccc tcactcagtc
     3001 gagtagcccg gcccatcgcc agaggctcgt tgatctctcg gtctcggggg agcaccaaac
     3061 gccctggaag tttcacgtcg ccagataatg cccgcactcg cgggcggtgt caggattttg
     3121 cgatcgggac gcgctccggc gggatcgaaa gtcggatcac aggtcgcgtc ggccgatcag
     3181 ccggcaggcg aggaccacta gcccgaccgt gagcacgagc gtcaccgccg tggcaccgag
     3241 gtagtcgcca ggtgtcttga ccccgcgagc cagcgcgtcc tgcgcgccga ccagcgccga
     3301 gggcagccag cggcccagcg gcgcccacac cccgacgatc ggcagcgcca gcaacgcccc
     3361 gagcgtgatc ccgaccgtgg ccagcacgct gcgcgagcgg ctcgccgcca aggcgacgac
     3421 tgcgacggcg aagaccaggt agagcatccc cagcagcccg cccatcgcga cgtcaagcgg
     3481 gtcgatcccg ccgagcagca ccacggtctc gtagaccgcg gccagcaacc cggccagata
     3541 ggccagtgcg gcggccgatg cggcgacgac aaatcgaggt acgacgagat cagcaggacg
     3601 ccgtacccgg gtgcggtaga acaccgccag ccccggccgg gcgtcgagcg tgagcgccag
     3661 cgcagcgacg atgaccacga cgacgagccc gatctggctg gcattgttga cgaatgcggc
     3721 cagtccgtcg gccggcaccg ggtcaggcgc gacgatcgtg atgtccccgc cggcccgggc
     3781 caagatctcg cggatgtagc gcgccgagag cggcccgagc agaccgaaca gcagataaac
     3841 ggccagcagc gcgacccagc gtcgggtgcg ccacagccgc agcagctcga cccgccacaa
     3901 cgtcgtacgg cgtgatggcg cggccgccga cgtgaccggc acgctcatcg agccaccgcc
     3961 gcgtgggtca gcgacaggaa tgcgctctcg aggtcggcct cgagcggggt cagcgacacc
     4021 acggccaggc cgagcgccgc ggcggtcgcc accacaccgt gctcaccctc ggccaaggtg
     4081 cgcgcctcca catgcaccgt ctgcccgtcg gccggctcgg cctggaccac ccacggctcg
     4141 gcgcccagcg cggcaaccag ccgttcggac ccggagcgca gccgcaccgc ccagcgcggg
     4201 cgcacgtgct cggccagcag ccggtcgagc ccaccttggt agagcagccg gccggcgcgc
     4261 agcacgccga cggtgtcgca gacccgttgc acgtcaccaa gaatgtgact cgagaagatc
     4321 acggtggtcc gcccgcgcat cccgaccacc aggtcgagga cctccgcgcg cccggccggg
     4381 tcgagcgccg aggtcggctc gtcgagcacg agcacctcgg ggtcgccgac cagcgccgcg
     4441 gccagcccga gccgctgggt catcccgcgc gagaacccgc cgacccggcg gtccgcggcc
     4501 tcggtcagcc cggcccggcc aagtgcctcg gcaacggcac gccgatcgga cggcccggtc
     4561 gacgcaccgt cggcgtccct gaccagcccg gcggccagct cgacgacttc ggtcgcggtc
     4621 agccagggct cgaactccgg cacgtcggcg cacaccgcga cccggcgccg gtccaccccc
     4681 agccggacct ctccgcggtc ggcgcggcgc aggccggtga cgattgacag caaggtcgtc
     4741 ttgcccgcgc cgttgggccc gaccagcccg tagacgctgc ccgccggcac cgcgaggtcg
     4801 acgtcgaaga tgccggcgcc ctgcccgtag tcgcgggtga gcccggtggt ggcgacgacc
     4861 ggcgcgctca ccgctcagcc cggccggcca gcaggaacac cacaccgccc aacggcaccg
     4921 acagcacgac gaccaacacc caggcccacc gcggcagccc gcgcacaccg ggcgcccgca
     4981 cgatctgcac caggcagaag gcgacccacg ccacggcgag caggatcagc ggggcgaggg
     5041 cggcccaaga gattccagcg gaagacatgc aggtccttta cgacgtacga cgaatctgac
     5101 gcgacgcaga ccgtaacccg gcgcaccccg gtcaggcgag cacgaagtag cgcaaccaga
     5161 cgtagggcac gcagatggcg atcgacacgg tcgcgaccac cagcccgtag cgggtgaact
     5221 cccagaacga gatcggggtg ccgttgcgct tggcgatgcc caggacgacg acgttggcgc
     5281 tggcgccgat cgcggtcgcg ttgccgccca ggtcggcgcc cagcgcgagc gaccaccaca
     5341 gcacagctcc cgcgccgaga ccaccggccg cgtcgacgag ctgctccaca agcggcgtca
     5401 tcgtggcgac gtaggggatg ttgtcgacga tccccgagag caccccggag acgacgagca
     5461 gcagcatcga ggcgacgaac acgctgccgc cggtcgcctc ggtggcccac gtggccagct
     5521 tctcgatggt gccgaccttg accagcgcgc cgaccatgat gaacaggccc atgaagaaga
     5581 cgagggtctc ccactcgacg tccgcgaggt agccggccgg ctccatcccc gacagcagca
     5641 cgagcagccc ggcgccggtg agcgcgacga ccgagggttc cagatgcagc gcggagtgca
     5701 gcacgaaacc ggcgagcacc agagccagca ccaccatgct gcgcaccagc agccgcgggt
     5761 tggtgatcgc ctcgcgctcg accaggctca tcacctcgtc gacccgctcg ggatgcgcga
     5821 cgaacgcctt gcggaacagc acccggcaca gcccgatgaa caccaccagg atgatcacca
     5881 cgaccggtgc gaggttgacg aggaagtcgt tgaacgacaa gcccgatcgg ccggcgatga
     5941 tgatgttggg cgggtcgccg atcagcgtcg ccgtaccgcc gatgttggag gccatcgcct
     6001 ccgcgatcag atagggcacg gggttgagaa gcaaccggtc gcagaccagc agcgtcaccg
     6061 gggcgaccag cagcaccgtg gtgacgttat cgagcgccgc cgaggccacc gcggtgatca
     6121 gcaccagcag caccatgacc cggaacggat cgcccttggc ccgcttggcc gcccagatcg
     6181 cgaggtagtc gaagatcccg gtctgcttga ccactccgac gatgatcatc atgccgagca
     6241 gcaggaagat gacgttccag tcgatgccgg tctcgcggct gaagaacgag tcgttgggac
     6301 ccacgacacc cagggcgagc acgacgagcg cgccgccgac cgccgccgcg acacggttga
     6361 ccttctccga ggcgatgagg acgtaggccg cgccaaagac ggcgattgcg agaggagcac
     6421 tcatacgtcg ggcagcagga tctcgagcag gcgagaggtg gtgaccgctc cgaggatctc
     6481 gtcgtgctcg gacaccacga tgagaggact gtgcaggcgg gccatcaacg cggcgatctc
     6541 cagcacctgg tcgtcaccgc gtacgacggg cagctcgcgc gcgccgtcgg gcagcagcga
     6601 ccgcaccgtg cgcccacgca gccgttcgaa gagctcggcc gcgtcgtcct cggtgaacac
     6661 ccgagccagc gtcgggtcgt cctgcacgta gtgcggaatg aggagtctca ccacctgcgt
     6721 gccgggcagg accgcgatcg gccggtgcgg gtcaccgtcg accacgatga tcccgggcag
     6781 ccggcgcacg gccatcgcgt gggctgcctc gtagacatcg gcgtcggcat ggacgaccgg
     6841 gtagtcctcg gcgatctccc gcgccagcat gcgcgcacct tatcgaccag caaacccacc
     6901 ccggccgagc ctgagcccag gcgggctggg gcgcggtgtc ggcgggatgg gtcacactga
     6961 tgggtcggcg tgacccgcgc ccatatccag ctgggggaag gacccgcgtg accgacggac
     7021 cgctcatcgt ccagagcgac aagactctgc tgctcgaggt cgaccatccg caggccgccg
     7081 aggcgcgccg cgagatcgcc ccgttcgccg agctcgagcg ctcccccgag cacgtgcaca
     7141 cctaccgggt caccccgttg gggctgtgga acgcgcgggc ggccgggcac gacgccgagc
     7201 aggtcgtcga cgcgctgctg cgctggtcgc gctacccggt gccgcacgcg ctgctcgtcg
     7261 acgtcgccga gacgatggac cgctacggcc ggctgcggct ggagaaggac cccgcccacg
     7321 gtctggtgct gatctccact gaccgggcgg tgctcgagga ggtgctgcgg tccaagaaga
     7381 tcgcccccct cgttggcgaa cgcgtcgccg aggacaccgt ggtcgtgcac cccagcgagc
     7441 gcggctcgct caagcaggtg ctgctcaagc tgggctggcc ggccgaggat ctcgccggct
     7501 acgtcgacgg cgaggcgcac ccgatcgcgc ttcgcgagga cggctgggcg ctgcgcccct
     7561 accaggccga ggcggccgaa ggcttctggc acggcgggtc cggggtcgtc gtactcccgt
     7621 gtggtgccgg caagaccatc gtcggggcgg ccgcgatggc ccgggcccag gcgaccaccc
     7681 tgatcctggt gaccaacacg gtggccgcgc ggcagtggcg gcacgagctg ctgcgccgta
     7741 cgtcgctgac cgaggacgag atcggcgagt actcgggcgc caagaaggag atccggccgg
     7801 tcaccatcgc gacctaccag gtgatgacca cccggcgcaa aggcgtctat gcccacctcg
     7861 agctgttcga ctcccgggac tggggcttga tcgtctacga cgaggtgcac ctgctgcctg
     7921 cgccgatctt ccggttcacc gccgacctgc aggcgcgccg tcggctcggg ctgaccgcca
     7981 ccctggtccg cgaggacggc cgcgagggcg acgtgttctc gctgatcggg cccaagcgct
     8041 acgacgcgcc ctggaaggac atcgaggccc agggctacat cgcgccggcc gactgcgtcg
     8101 aggtgcgggt gaccctgtcc gagcgcgagc ggatgctcta tgccaccgcc gagccggagg
     8161 agcgctatcg gctggccgcg agctcggaca ccaagaaccg catcgtcgag gcgctggtgg
     8221 ccgcccacgc cggcgagccc accctggtga tcggccagta catcgaccag ctcgacgagc
     8281 tcggcgagca cctcggcgcc ccggtgatca agggcgagac cacggtgcgc gagcgcgagc
     8341 ggctcttcga ggcgttccgc accggcgagc tgagcaccct ggtcgtgtcc aaggtcgcca
     8401 acttctcgat cgacctgccc gaggcgtcgg tggcgatcca ggtctcgggc acgttcggct
     8461 ctcggcagga ggaggcccag cggctgggtc gggtgctgcg ccccaaggcc gacgggcgcg
     8521 gcgcgcggtt ctatgccgtg gtcgcccgcg acaccctcga ccaggactac gcggcgcacc
     8581 ggcagcgctt cctggccgag cagggctacg cctaccggat catcgactcc gacgacgtgc
     8641 ttcgcggcga cctcaccggc gacggccccg ccgtcgactg atcgatcgcc ccgcacccgt
     8701 cgagggtctc gatgcgcgag acggacgggc cgcgctcggt ctgcagcctc gccgcgacga
     8761 cctcggccga gccgagcaca cgcatgacgt tgccgtgcgt caggcgtcgt acgtcgtccc
     8821 cggtccagcc ccgttcgagc agctcggcca ccagcgtcgg gtagcccgtg acgtcctcca
     8881 tgccgagcgg ctgcacgtcc accccgtcgt agtccccgcc cagcccgacg tggtcgatgc
     8941 ccgccacctc gcgcacatgg cagacgtggt cggcgacgtc ggcagccgag gcccgcggcc
     9001 gcggcgagcg cgcgtccacc tcggcggcga cccgcatccg ggcctgccag tcgccgggcg
     9061 gcaggccctg gcgcaccatc tcggcccgca ccttctcgtc ccaggcccga cacggctcgg
     9121 agacgaacgc cggcacgaac gtcaccatgc acactccgcc gttggccggc agcgacgcga
     9181 gcacgtcgtc gggcacgcag cggacgtggt cgcagaccgc gcgggccgag gagtgcgaaa
     9241 acaccaccgg cgccgcggcg accatcaacg cgtgccgcat cgtgtcgggc gagacgtgcg
     9301 agaggtcgac gagcatgccg agccggttca tctcgcgcac cacttcttcg ccaaaccggg
     9361 tgagcccacc gacccgcggc gagtcggtgg ccgagtcggc ccacggcacg ttgtcgttgt
     9421 gcgtcaaggt catgtagcgc gcgcccagcg catgcatcat gcgcagcgcc gccagcgagg
     9481 aatcgatgct gtgtccgccc tccatgccgg ccagcgacgc caaccgaccg gcggccatcg
     9541 cctgcgacac ctcggctgac gacgtgcacc aggcgaacga ccccggccag cgcgcgcaca
     9601 tgcgtcgtac gacgtcgagc tgctcgagcg tcgcggtcac cgcgctcgag ccaggcaacg
     9661 tcgacgggac gtagaccgac cagaactgcc cgccgacgcc cccggcccgc aaccggggcc
     9721 agtcggtg
//
LOCUS       DATIBB010000133        15869 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_734402, whole genome shotgun sequence.
ACCESSION   DATIBB010000133 DATIBB010000000
VERSION     DATIBB010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 15869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15869)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15869
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_734402"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..392
                     /gene="recA"
                     /locus_tag="VLC50_05090"
     CDS             <1..392
                     /gene="recA"
                     /locus_tag="VLC50_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217253.1"
                     /note="these RecA proteins contain inteins; catalyzes the
                     hydrolysis of ATP in the presence of single-stranded DNA,
                     the ATP-dependent uptake of single-stranded DNA by duplex
                     DNA, and the ATP-dependent hybridization of homologous
                     single-stranded DNAs; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="intein-containing recombinase RecA"
                     /protein_id="HSF26876.1"
                     /translation="AAVQSVEPAGRIATFDLEVDELHNLVAEGVVVHNCSPPFKQAEF
                     DILYGMGISREGGLIDLGVEHGLVRKSGAWYTYEGDQLGQGKENARNFLRDNPDLADE
                     IEKKIKEKLGIGPRLDAEPDAAEPTGF"
     gene            403..972
                     /locus_tag="VLC50_05095"
     CDS             403..972
                     /locus_tag="VLC50_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013116778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HSF26877.1"
                     /translation="MQRRAAGGSRRRRGPDGPLPGGAAADMPDADPETVARAIVLRRL
                     TAAPRSRADLAATLAERDVPDDVAKRVLDRFEELALVDDAAYAEMLVRSRRATRGLAR
                     SALARELRQHGIAEQVADAALAGLDPDDELDTARELVQRRLSATRGLDRPARVRRLAA
                     MLARKGYPPGLCLRVVTEVLGAEIDENSV"
     gene            1317..2924
                     /gene="rny"
                     /locus_tag="VLC50_05100"
     CDS             1317..2924
                     /gene="rny"
                     /locus_tag="VLC50_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020541758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HSF26878.1"
                     /translation="MSALVVGSLVTLALLVVVLLVVLTVAVLRRPEPAPVAPVDALGR
                     PLAEVEDARREAAELVERARRDAETLRRDADLSVEHARTLREGAEAEVRVLRDELRSD
                     RAEIERREHRAIERESRLDADARLLDERARELAAAEAALAERRAELANLEEERRLVLE
                     RTAGLTAEAAKAELIAVIENQAKREAAVLVRDIEAEARGAGEARARHIVAESIQRVAS
                     EQTAESVVSVLHLPNDEMKGRIIGREGRNIRAFEQLTGVNLIVDDTPEAVLLSCFDPV
                     RREVARVTLESLVLDGRIHPHRIEEVYERSKSTVDELCRRAGQDALVQVGITDLHPDL
                     VDLLGRLRYRTSYGQNVLKHLVESAHVAGLMAAELGVAPALVKRGAFLHDIGKALTHE
                     AEGSHAIVGAELARRYGEPEEVVHAIEAHHDEVAPQTVEALLTQAADACSGGRPGARR
                     ESLEVYIARLQRLEEIAGAHEGVEKVFAMQAGREVRVMVLPDVVDDIQTQVLARDIAK
                     QVEEELTYPGQIRVTVIRESRATETAR"
     gene            2930..3592
                     /locus_tag="VLC50_05105"
     CDS             2930..3592
                     /locus_tag="VLC50_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018523669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HSF26879.1"
                     /translation="MSDGLRVGDPEALLDRAGALARAGSRRLLGITGPPGAGKSTLAA
                     ALADRLAGAAVVVPMDGFHLAQAELIRLGRAARKGAPDTFDALGYVALLRRLRAGSEP
                     VYAPAFDRRLEEPVAGAIAVDPAIGLVITEGNYLLLDGPWAPVRQLCDEIWYVDLPDD
                     QRLARLVARHAAHGKSLAAARAWANGSDAVNAALVVATRDRADVLVRCSGQSIARSVT
                     NP"
     gene            complement(3559..4197)
                     /locus_tag="VLC50_05110"
     CDS             complement(3559..4197)
                     /locus_tag="VLC50_05110"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HSF26880.1"
                     /translation="MPNALRGTDWTHLPTSRKVVALTFDGGSSDTAVGSILRTLDDKD
                     VPATFFLTGDFARRYPAQARRIAAAGHVLGNHSNTHPDFTTLTNAAIRAQLAAAEAAI
                     RSAAGASATPFFRFPFGARAAADIAVVNSAGYVPIRWTVDTLGWKGTSGGITAAVVVD
                     RVLARLRAGQIVLMHVGANPDDGSALDAAALPRVIDELRARGYGFVTLRAML"
     gene            complement(4560..5426)
                     /locus_tag="VLC50_05115"
     CDS             complement(4560..5426)
                     /locus_tag="VLC50_05115"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HSF26881.1"
                     /translation="MSQPTSVLFDVPGPRAQRRNNGVGIIGATIVVGLLLLVIWRAWS
                     KGAITPDRFSAYTDPELLNGLLEAILNTIKAAGAAILLALVFGAVFCLLRLSDRAWIR
                     WPAVAVIEFFRATPLLLLILFLYLGFAETLGVYGALVVGLMLYNGSVLAEIFRAGVAA
                     VPRGQSEAAYAIGMRKSQVLWLILVPQAVRAMLPAIISQCVVALKDTSLGFVIGYAEL
                     ARFNKLVYTAENNILASTIVVTAIYIAMNFGLSRVAVWLERRQSRGGGPSLGPDMHVE
                     APRIEGSPNLVP"
     gene            complement(5423..6070)
                     /locus_tag="VLC50_05120"
     CDS             complement(5423..6070)
                     /locus_tag="VLC50_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019876545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HSF26882.1"
                     /translation="MDVLIDKWPQVLAGFWTTLQLLAISGLIATVLGTVLAAMRVSPV
                     PVLRAAGTLYVNIFRNTPLVLLFTLVSFGLPTVEIRFSFFAFAIIALSAYTAAFVCEA
                     LRSGVNAVHTGQAEAARALGMTFGQTLRIVVLPQAFRTVIPPLASIYIALTKNTAIAE
                     AFAVTEATYQLDSLVRDFPNVLYYLFFGIALGYVIISWTIALAARLFERKLAVAR"
     gene            complement(6178..7059)
                     /locus_tag="VLC50_05125"
     CDS             complement(6178..7059)
                     /locus_tag="VLC50_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007077700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate ABC transporter substrate-binding
                     protein"
                     /protein_id="HSF26883.1"
                     /translation="MRMRRSRLAAMLVASFTAVTLVAACGGSSEGSDVNVNTDATFAA
                     GSTMDSLQKAGKITIGVKVDQPGIGYQKPGADTPEGFDIEIGKIIAAGLGIEPGDIEW
                     KETVSKNREPFIQNGTVDIVIASYSITDKRKEVVGFAGPYYVTGQQLLVRADDASIKG
                     PDDLDGKKVCSVTGSTSIKTVEEKYGAQPAPYSTYSECVQQLDNKVVDAVTTDGAILL
                     GYAAEDPDNRKVVGDAFSEERYGVGLKKGDTQFCQFITDTLKGAQDDGSWAKAFEATL
                     GKSGVETPAPPAMDACS"
     gene            complement(7120..7938)
                     /locus_tag="VLC50_05130"
     CDS             complement(7120..7938)
                     /locus_tag="VLC50_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006591750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HSF26884.1"
                     /translation="MSDATPEMADDAAPDAAGVTSPASRATGRPLVVLDKVNKHFGDL
                     HVLRDVDLTIHEGEVLVVIGPSGSGKSTLCRAINRLEPIESGTISLDGRPLPAEGRQL
                     ARLRSEVGMVFQSFNLFAHKTILQNVTIGPIKVRGTDPAVAEKSAMALLERVGVANQA
                     RKYPAQLSGGQQQRVAIARALAMDPKVMLFDEPTSALDPEMINEVLDVMTTLARDGMT
                     MVVVTHEMGFARRAADRVIFMSDGQIVEQASPEEFFTHPKSERAKDFLSKILAH"
     gene            8072..9550
                     /gene="miaB"
                     /locus_tag="VLC50_05135"
     CDS             8072..9550
                     /gene="miaB"
                     /locus_tag="VLC50_05135"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006130022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HSF26885.1"
                     /translation="MSTRTYEVRTYGCQMNVHDSERIAGLLEESGYLRAPEGQQADVV
                     VFNTCAVRENADNRLYGNLGHLAPVKADRPGMQIEVGGCLAQKDRGEILRRAPWVDVV
                     FGTHNIGSLPVLLERARHNDEAQLEILESLERFPSTLPTRRESPYAAWVSISVGCNNT
                     CTFCIVPSLRGREKDRTPAEILAEVQALVAEGVIEVTLLGQNVNSYGVEFGDRAAFAK
                     LLRACGQVEGLERVRFTSPHPASFTDDVIEAMAQTPNVMPQLHMPLQSGSSRLLKAMR
                     RSYRKERYLGIIERVRAAMPDAAITTDIIVGFPGETDEDFEHTLDVVRQARFAGAFTF
                     QYSKRPGTPAAEMDEQVPKEVVQERYERLVALQDEIAWAENQALVGRTVEVLVAEGEG
                     RKDEATHRLSGRAADNRLVHVAVPDGAERPRPGDMVTVEVTHAAPHHLVADSGLSGGA
                     FAVRRTRAGDAWEGRLRRTPVTAARPSAQAVLAAAVVGSPPR"
     gene            9547..10257
                     /locus_tag="VLC50_05140"
     CDS             9547..10257
                     /locus_tag="VLC50_05140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF26886.1"
                     /translation="MSDRTSRWEIPGDDPSSRRRPAREPKRRPVLRAVPPTIGQGPDP
                     AARMLAGEDAAGQIVRAGHAEHADVEHLVALADEVGLDALAALWSRSEPGTLPAALWA
                     LYAMRSWCRARPRELARLVTAGRAHAEVAAGVAGLPEDVDEADIAALGDDVVRGAVSR
                     DLATALARAAAFARVVAAGRGASGDDAVAMTAAHSRAAWAEAVMGSRLLGTAEALDRA
                     AARAPGLTSDDHEGGRDS"
     gene            10322..11056
                     /locus_tag="VLC50_05145"
     CDS             10322..11056
                     /locus_tag="VLC50_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004945366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF26887.1"
                     /translation="MPVFVAGAVCPHPPVLVPELAGGAVGELDELRRACGHAVDAMLA
                     ARPDLVVVVGRSDDDHGDEIRGLPAGSWGALTPYGVPVHVRPPGTDRDRAPELPLSLT
                     IGAWLLGRAGWAGPTAWITTGPEPSRPRVEACGRELAARAGRVAVLAMGAGSARRLAT
                     GPGGLDPAAPAYDELVTTALGAADTATLLGLDSAEGARLLVTGLGPWQVLAAAAEAGA
                     QAPSAQLLHAATPYGVTYLVASWVTG"
     gene            complement(11069..11284)
                     /locus_tag="VLC50_05150"
     CDS             complement(11069..11284)
                     /locus_tag="VLC50_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018547387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HSF26888.1"
                     /translation="MGMFDEMKDKAAKLAREHGEKIDDAIEKAGDVVDDKTGGKHSDK
                     IDAAQQKAREAADKLSRDNPDRPSQPR"
     gene            11375..12292
                     /gene="miaA"
                     /locus_tag="VLC50_05155"
     CDS             11375..12292
                     /gene="miaA"
                     /locus_tag="VLC50_05155"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011291262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HSF26889.1"
                     /translation="MQTPPPPIVAVVGPTAAGKSDLAVDLALRLGGEVVNADSMQVYR
                     GMDIGTAKLTPTEQRGVAHHLLDLWDVREPASVAVYQRLARACAAELGAAGIRPVLAG
                     GSGLYVRAVLDDLDFPGTDPAVRARLEAELAEAGPAPLHERLARLDPLAAARILPSNG
                     RRIVRALEVVQATGRPFSATLPVPRYVVPAVQIGLEVPREALDARIERRVDRMWEQGL
                     VEEVRRLAAAGLREGRTASRALGYAQVLRHLSGEWTEQQARDETVRATRRFARRQQSW
                     FRRDPRVHWLPYDAPDLLDRALALLAPDR"
     gene            complement(12293..13378)
                     /locus_tag="VLC50_05160"
     CDS             complement(12293..13378)
                     /locus_tag="VLC50_05160"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HSF26890.1"
                     /translation="MSEPAETPFGTETEVEVPRRGWLSWRGGLQTLVGAGVAVALIVW
                     ALPKVTGSSWPETAEALERVSLAAGLGMFALLLLAMWCYTFVLSASLPGLSHYKALIV
                     NVAGSSVSNLLPFGGAVGVGITYGMCRSWGFSRSAIVLSAVVSGVWNTLAKLVMPALG
                     LAALLLGGEIVTRRLAVAAGVAVAVLLLIVAVATTVLASERAALAVGRGAARVGELAL
                     RLVRSHRHLHWDSAVIALRHRTTDLVRAAWVRMTLGMTAFVAVYAVLFWMCLRAVGAD
                     PTLPQVFAAYTLGRLLTSVVVTPGGLGISETGAAALLVALGVPAAPATAGVLLFAFYV
                     HVLEVPLGALGWLAWSLTKRTTPSATR"
     gene            complement(13375..14232)
                     /locus_tag="VLC50_05165"
     CDS             complement(13375..14232)
                     /locus_tag="VLC50_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013424043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HSF26891.1"
                     /translation="MVVFHAHPDDESLLTAGTMARAAAAGHRVVLVVATAGEAGLASS
                     DLVVDGRLGQRRLRELHRSAAALGVARVEVLGYGDSGLAPDVERPVVSEAFAGRTLFA
                     HADVEEAAARLAEILCEEQADVLTSYDANGGYGHPDHVQVHRVGLRAAALAKTPALLE
                     ATVDRDLLLRGLALASKVHRFPPEFDFASFQQAYVPRTAITHRVDVRRYARAKRASMA
                     AHASQATADGGDRTLAALLRLPQPVFRWVLGREWFTQVRPAVPSGSRLLRDPFAAVGT
                     SRRRGPRPG"
     gene            14308..15459
                     /locus_tag="VLC50_05170"
     CDS             14308..15459
                     /locus_tag="VLC50_05170"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019136415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HSF26892.1"
                     /translation="MKIAHLTDCYLPRLGGIEVQVHDVATRQLAAGHTVEVLTATQGE
                     TRVRGRSRDLVDGVPVHRLSMRVPFELPVNPWAAAAVRTVLAEGGFDVAHVHAGVISP
                     FAVDAARVSVGLGVPTLVTWHCLLGGLEPVFRLLERGTGWARRPMALSAVSDVAAAPL
                     RRIAGPGAEVAVLPNGIDVETWRVVPAARAEQTVHVVATMRLAPRKRPIPLLRILRDV
                     RRLVPDDIRLRATIVGDGPALAPARRYVDRHGLGSWVRLLGRLRRSQIRELYRDADVF
                     VAPAELESFGIAALEARCAGLPVVAKAVSGIREFVHDGQEGLLAGSDLEMADALVRLV
                     TDAELRARIAAHNRAVEPDMSWPRVLKRTEEEYLRAIAIMAFHRDRGRA"
     gene            15490..>15869
                     /locus_tag="VLC50_05175"
     CDS             15490..>15869
                     /locus_tag="VLC50_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018818020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HSF26893.1"
                     /translation="MVFVKGHGTQNDFLVLPDPDGTLALSARLVSALCDRRRGLGADG
                     VLRVVRVEAAGPDAADAVGAAEWFMDYRNADGSLAEMCGNGVRVYARYLVESGLASGP
                     RLPILTRAGLVVAEIGRETIAVQMP"
BASE COUNT         2181 a         5655 c         5879 g         2154 t
ORIGIN      
        1 tcgccgcggt gcaatccgtt gagccagcgg gtcggatcgc gacgtttgat ctcgaagtgg
       61 acgaactaca caatctcgtt gcggaggggg ttgtcgttca caactgcagc ccgccgttca
      121 agcaggccga gttcgacatc ctctacggca tgggcatctc ccgcgagggc gggctgatcg
      181 acttgggcgt cgagcacggc ctcgtgcgca agtccggtgc ctggtacacc tacgagggcg
      241 accagctcgg ccagggcaag gagaacgccc gcaacttcct gcgcgacaac cccgacctcg
      301 ccgacgagat cgagaagaag atcaaggaga agctcggcat cggcccgcgt ctggacgccg
      361 agcccgacgc ggccgagccc acaggcttct aggccggtgc cggtgcagcg gcgagccgcc
      421 ggagggtcgc gtcgtcggcg aggacccgac gggccgctgc ccggcggcgc ggccgccgac
      481 atgcccgacg ccgacccgga gacagtcgcc cgggcgatcg tgctgcgccg gctgaccgcc
      541 gccccccgca gccgggccga cctggctgcc acgctggccg agcgcgacgt ccccgacgac
      601 gtggccaaac gggtgctcga tcggttcgag gagctcgcgc tggtcgacga cgcggcctat
      661 gccgagatgc tcgtgcgctc gaggcgggcc acccgcgggc tggctcggtc agcgctggcc
      721 cgcgagctgc gtcagcacgg catcgccgag caggtcgccg acgccgcgct cgccgggctc
      781 gaccccgacg acgagctcga caccgcccgc gagctcgtcc agcgtcggtt gagcgcgacc
      841 cgggggctcg accgccccgc acgagtgcgc cggctggccg cgatgctggc tcgcaagggc
      901 tacccgcccg ggctgtgcct gcgcgtcgtc accgaggtcc tgggcgccga gatagacgag
      961 aacagcgtgt gatgttgacc atttcgtgac ctcggcctag cctcggtgca agagggaaac
     1021 acgccgtgac ctgcggctgt gcaccaccga acgaccacag cacgacccag agcgcacacg
     1081 atctcgacga caccgcctga cccaccggca tcctgccggt gactgccctg atcgaccgtt
     1141 acaccgggca ccgaccccgg gtctttggcg acggcacgtc gtcgcgacgg ccccagcggt
     1201 ggccgcgccg ggacctcccg gcggtgagcc gtcggcggtg ttctgccgat gggcggcgtg
     1261 gtccctcccg cgtgcagccg gacgcccggc tgccaggcct gagggagggg atgacgatgt
     1321 ccgcactcgt tgtcggatcg ctggtcaccc tggcccttct cgtggtggtg ctgctcgtcg
     1381 tgctcacggt cgcggtgctg cgccggccgg agccggcgcc cgtggctccc gttgacgcgc
     1441 tggggcgccc gctcgccgag gtcgaggacg cccgccggga ggccgccgaa ctggtcgagc
     1501 gggcccgccg ggacgccgag accctgcggc gtgacgccga cctcagcgtc gagcacgccc
     1561 gcacgttgcg ggagggtgcc gaggccgagg tgcgcgtcct gcgagacgag ctgcggtctg
     1621 accgggccga gatcgagcgc cgagagcacc gggccatcga acgcgagtcg cgcctcgacg
     1681 ccgacgcccg gctgctcgac gagcgggcac gcgagctggc cgccgcagag gctgcgctcg
     1741 ccgagcggcg ggccgagctg gccaatctcg aggaggagcg tcgcctggtg ctcgagcgca
     1801 ccgcgggcct gaccgccgag gcggccaagg ccgagctcat cgcggtgatc gagaaccagg
     1861 ccaagcgtga ggcggccgtg ctcgtgcgcg acatcgaggc cgaggcccgc ggcgccggcg
     1921 aggcgcgggc tcgccacatc gtggccgagt cgatccagcg ggttgcctcc gagcagaccg
     1981 ccgagtcggt cgtctcggtg ctgcacctgc ccaacgacga gatgaagggc cgcatcatcg
     2041 gccgcgaggg ccgcaacatc cgggcgttcg agcagctcac cggcgtcaac ctgatcgtcg
     2101 acgacacgcc cgaggcggtg ctgctgtcct gtttcgaccc ggtccgccga gaggtcgccc
     2161 gggtgacgct cgagtccctc gtcctcgacg ggcggatcca cccgcaccgc atcgaggagg
     2221 tctacgagcg cagcaagagc accgtcgacg agctgtgccg gcgcgcgggc caggacgcgc
     2281 tcgtgcaggt gggcatcacc gacctgcacc ccgacctggt cgacctgctc ggccggctgc
     2341 gctaccgcac ctcctacggg cagaacgtgc tcaagcacct ggtcgagagc gcccacgtgg
     2401 ccgggctgat ggccgccgag ctcggcgtgg cgcccgccct ggtcaagcgg ggagccttcc
     2461 tgcacgacat cggcaaggcg ctgacccatg aggccgaggg ctcgcacgcc atcgtggggg
     2521 ccgagctggc ccgccgctac ggcgagcccg aggaggtggt gcacgcgatc gaggcccacc
     2581 acgacgaggt ggccccgcag accgtcgagg cactgctgac ccaggcggcc gacgcctgct
     2641 cgggcgggcg gcccggggcg cggcgggagt cgctggaggt ctacatcgcc cggctccagc
     2701 ggctcgagga gatcgccggc gcccacgagg gcgtggagaa ggtgttcgcg atgcaggccg
     2761 ggcgcgaggt ccgcgtcatg gtgctgcccg acgtcgtcga cgacatccag acccaggtgc
     2821 tcgcccgcga catcgccaag caggtcgagg aagagctgac ctaccccggc cagatccggg
     2881 tcaccgtcat ccgggagtcc cgggccaccg agaccgcccg ctgacctgcg tgagcgatgg
     2941 gttgcgggtc ggcgacccgg aggcgctgct cgaccgggcc ggggcgctgg cccgcgccgg
     3001 gtcgcgccgg ctgctcggga tcaccggccc gccgggcgcc ggcaagtcga cgctggccgc
     3061 cgcgctcgcc gaccgcctcg ccggcgcggc ggtcgtcgta ccgatggacg ggttccatct
     3121 cgcccaggcc gagctgatcc ggctcggtcg tgcagcgcgc aagggcgcgc ccgacacgtt
     3181 cgacgcgctc ggctacgtcg ccctgctgcg gcgtctgcgg gcggggtccg agccggtcta
     3241 cgcgccggcg ttcgaccgcc gcctcgagga gccggtcgcc ggcgccatcg ccgtcgaccc
     3301 ggcgatcggg ttggtgatca ccgagggcaa ctacctcctg ctcgacggtc cgtgggcgcc
     3361 ggtgcggcag ctctgcgacg agatctggta cgtcgacctg cccgacgatc aacgcctcgc
     3421 gcggctggtg gcgcgccacg ccgcccacgg caagagcctg gcggccgccc gcgcctgggc
     3481 caacggctcc gacgcggtca acgccgcgct cgtggtcgcc acccgggacc gggccgatgt
     3541 gctggtgcgc tgcagcggtc agagcatcgc gcgcagcgtg acgaacccgt agccgcgggc
     3601 gcgtagctcg tcgatgaccc gcggcagggc cgcggcgtcg agcgcgctgc cgtcgtcggg
     3661 gttggctccc acgtgcatga gcacgatctg gcccgcgcgc aggcgtgcga gcacccgatc
     3721 gaccaccacc gcggcggtga tcccgccgct ggtgcccttc cagcccagcg tgtcgacggt
     3781 ccatcggatc ggcacgtagc cggcggagtt gaccacggcg atgtcggccg ccgcgcgagc
     3841 cccgaacgga aaccggaaga acggcgtggc gctcgcgccg gcggcgctgc ggatcgcagc
     3901 ctccgcagcc gcgagctggg cgcggatggc cgcgttggtg agcgtggtga agtcggggtg
     3961 ggtgttgctg tggttgccga gcacgtgccc ggcagcggcg attcgccgcg cctgggccgg
     4021 gtagcgacgc gcgaagtcgc cggtgaggaa gaacgtcgcc ggcacgtcct tgtcgtcgag
     4081 tgtgcgcagg atcgatccga ccgcggtgtc gctcgagccg ccgtcgaagg tcagggcgac
     4141 gaccttgcgg ctggtcggca agtgggtcca gtcggtgccg cgcagcgcgt ttggcacgcc
     4201 cgacggtggt gtcaccgatg ggttcgaagt gggacggcgg ccggtcggcg cggcgctggc
     4261 gggccggctc ggcggtgctg gtggagtcga cggagggggt gcggcggtga ccagccccga
     4321 cctggtcgtg gtcgggctcg gggcgggcgg ggtgaatgtg gagaccgtgg ggttgggcga
     4381 ggcggcgggc acggcgctgg gggacccgct gggggtgccg ctgcagccgg tgggcagcac
     4441 gagcacaacc atcgcgagcc tcgcgaggct ggttctccgc cccatagccg gagtctgcgg
     4501 gcattcctcg cgccctgcca gggacctagg tcgtggcgcg ggccgtgctg ggaggggaat
     4561 cagggcacaa ggttgggact gccctcgatg cgcggtgcct caacatgcat gtctgggccc
     4621 aggctggggc cgccgccgcg cgactggcgc cgctcgagcc acaccgcgac ccgcgacagc
     4681 ccgaagttca tggcgatgta gatggcggtc accacgatgg tcgaggcgag gatgttgttc
     4741 tcggcggtgt agaccagctt gttgaaccgg gccagctctg cgtagccgat cacgaagccc
     4801 agggacgtgt ccttcaacgc caccacgcac tggctgatga ttgccggaag catggcccgc
     4861 acggcctgcg gcaccaggat cagccagagc acctggctct tgcgcatgcc gatggcatag
     4921 gccgcctcgg actgcccgcg gggcaccgcg gcgacgccgg cccggaagat ctcggccagg
     4981 accgaaccgt tgtagagcat cagtcccacg accagggcgc cgtagacgcc gagcgtctcg
     5041 gcgaagccca ggtagaggaa gaggatcagc aacagcagcg gggtggcgcg gaagaactcg
     5101 atgaccgcga ccgcgggcca tcggatccac gccctgtccg acaaccgcaa caggcagaac
     5161 acggctccga agaccagggc gagcaagatt gctgcaccgg ccgccttgat ggtgttgagg
     5221 atcgcctcga gcaggccgtt gagcagctca gggtcggtat aggccgagaa gcggtccggc
     5281 gtgatcgccc ccttgctcca cgccctccag atgaccagca gcaggaggcc gacgacgatc
     5341 gtggcgccga tgatcccgac gccgttgttg cggcgctggg ctcgcgggcc ggggacgtcg
     5401 aagagcacgc tggtgggttg gctcatcggg ccaccgccag cttccgctcg aacagccgcg
     5461 ctgcgagagc gatggtccag gagatgatga cgtagccgag ggcgattccg aagaacaggt
     5521 agtagagcac gttcgggaag tcgcgcacca ggctgtcgag ctggtaggtc gcctccgtca
     5581 cggcgaacgc ctcagcgatg gcggtgttct tggtcagcgc gatgtagatg ctcgccagag
     5641 gcgggatgac ggtgcggaag gcctggggga gtacgacgat gcgcagcgtc tgaccgaagg
     5701 tcatgcccag cgcacgcgcg gcctcggcct gaccggtgtg cacggcgttg acaccgctgc
     5761 gcaaggcctc gcacacgaac gcagcggtgt aggccgacag cgcgatgatc gcgaaagcga
     5821 agaacgagaa ccggatctcg acggtgggca gcccgaatga caccagcgtg aacaggagca
     5881 ccagcggggt gttgcgaaag atgttgacgt aaagggtgcc ggccgctcgc agcaccggga
     5941 ccggagagac ccgcatcgcg gcgagcactg tcccgagcac cgtggcgatg agcccggaga
     6001 tcgccagaag ctgcagggtg gtccagaacc ccgccaacac ctgaggccat ttgtcgataa
     6061 gaacgtccac ctggctcctc gtgtcctcgc gtgaaactca ggcccaggac cgtgcggttc
     6121 gggatacgag cctgtgcggg cccggcgccg tgacgacgcc gggccccgag tcgtcggtca
     6181 ggagcaggcg tccatcgccg ggggagcagg cgtctcgacg ccggacttgc ccagcgtcgc
     6241 ctcgaacgcc ttggcccagg acccgtcgtc ctgggccccc ttgagggtgt cggtgatgaa
     6301 ctggcagaac tgggtgtcgc ccttcttcag gccgacgcca tagcgctcct cggagaacgc
     6361 gtcacccacc accttgcggt tgtcggggtc ctctgccgcg tagcccagca ggatggcgcc
     6421 gtcggtggtc accgcgtcga cgaccttgtt gtccagctgc tgcacgcact cggagtaggt
     6481 gctgtaggga gccggctggg cgccgtactt ctcctcgacg gtcttgatcg aggtcgagcc
     6541 ggtcaccgag cagaccttct tgccgtcgag gtcgtccggg cccttgatgg aggcgtcgtc
     6601 cgcgcggacc agcagctgct ggccggtgac gtagtagggg ccggcgaagc cgacgacctc
     6661 cttgcgcttg tcggtgatcg agtaggaggc gatcacaatg tcgacggtgc cgttctgaat
     6721 gaacggctcg cggttcttcg acaccgtctc cttccactcg atgtcgccag gctcgatgcc
     6781 gagacccgct gcgatgatct tgccaatctc gatgtcgaaa ccttcgggcg tatcggcgcc
     6841 gggcttctgg tagccgatcc ccggctggtc caccttgacc ccgatggtga tcttgcctgc
     6901 cttctgcagc gagtccatcg tggagccggc ggcgaacgtg gcgtcggtgt tgacgttgac
     6961 gtcactgcct tcggagctgc cgccgcaggc ggcaacgagc gtgactgctg tgaacgacgc
     7021 gaccagcatc gcggccagcc tggaccttcg catccgcatg gagcttcctc tctctgttcg
     7081 ccacctgccc cctggcggtg gtcggttcac gggtcggaat cagtgcgcga ggatcttgga
     7141 caggaagtct ttggcccgct cgctcttggg atgggtgaag aactcctccg ggctggcctg
     7201 ttcgacgatc tgaccgtccg acatgaagat gacccggtct gccgcgcggc gggcaaaccc
     7261 catctcgtgg gtgacgacga ccatggtcat gccatctcta gcgagtgtcg tcatcacatc
     7321 gagcacttca ttgatcatct cggggtccag cgccgaggtc ggctcgtcga acaacatcac
     7381 cttggggtcc atcgccaggg cccgggcgat ggctacccgc tgctgctgcc ccccggagag
     7441 ctgagccggg tatttgcggg cctggttggc cacaccgacc cgctcgagca gtgccatggc
     7501 actcttctcc gcgacggccg ggtccgtgcc gcgcaccttg atcggcccga tcgtgacgtt
     7561 ttgcaggatc gtcttgtgcg caaacaggtt gaacgactgg aacaccatcc cgacctcgga
     7621 gcgcagccgg gccagctggc ggccctcggc gggcagagga cgaccgtcaa gactgatcgt
     7681 gccggactcg atcggctcga gccggttgat ggcccggcag agcgtcgact tgccggaccc
     7741 ggacgggccg atgaccacca agacctcgcc ctcgtggatc gtcaggtcca cgtcgcgcaa
     7801 aacgtgcagg tcgccgaagt gcttgttgac cttgtcgagc acgacgaggg gtcgacctgt
     7861 ggcccgggag gccggcgagg tcacgccggc ggcatcgggg gcggcgtcgt cggccatctc
     7921 gggcgtcgcg tctgacatgc acggcaaact atgccttgac ccgctctggg gtcacgccag
     7981 ccaacgtcac ggtccgggca cagtgcgtgt cagtcgggtg cggccgggat ggccgggcgg
     8041 gccgggcgca gcccgtaccc tggacgacgt tatgagcaca cggacctatg aggtgcgcac
     8101 ctacgggtgc cagatgaacg tgcacgactc cgagcggatc gccggtctgc tcgaggagtc
     8161 gggctacctg cgcgcgcccg aggggcagca ggccgacgtc gtcgtgttca acacctgcgc
     8221 ggtgcgcgag aacgccgaca accggctcta cggcaacctc ggccacctcg ccccggtcaa
     8281 ggccgaccgg cccggcatgc agatcgaggt cggcggctgc ctggcccaga aggaccgcgg
     8341 cgagatcctg cgccgggcgc cgtgggtcga cgtggtgttc ggcacccaca acatcgggtc
     8401 gctgccagtg ctgctcgaac gggcccggca caacgacgag gcccagctgg agatccttga
     8461 gtcgctcgag cggttcccgt cgacgctgcc gacccggcgc gagtcgccgt acgccgcctg
     8521 ggtctcgatc agcgtgggct gcaacaacac ctgcacgttc tgcatcgtgc cgtcgctgcg
     8581 cgggcgcgag aaggaccgca ccccagccga gatcctcgcc gaggtgcagg cgctggtcgc
     8641 cgagggcgtc atcgaggtca ccctgctcgg gcagaacgtc aacagctatg gcgtcgagtt
     8701 cggcgaccgg gccgcgttcg ccaagctgct gcgcgcctgc gggcaggtcg aggggctcga
     8761 gcgggtccgg ttcacctcgc cgcacccggc ctcgttcacc gacgacgtga tcgaggcgat
     8821 ggcccagacc cccaacgtca tgccgcagct gcacatgccg ctgcagtcgg gctcatcgcg
     8881 gctgctcaag gcgatgcgcc gctcctaccg caaggagcgc tacctcggca tcatcgagcg
     8941 ggttcgcgcg gcgatgcccg acgcggcaat caccaccgac atcatcgtcg ggttccccgg
     9001 cgagaccgac gaggacttcg agcacaccct cgacgtggtg cgccaggccc ggttcgccgg
     9061 cgcgttcacg ttccagtact ccaagcgtcc cggcaccccg gccgccgaga tggacgagca
     9121 ggtgcccaaa gaggtcgtgc aggagcgcta cgagcggctg gtcgcgctgc aggacgagat
     9181 cgcctgggcc gagaaccagg cgctggtcgg gcgcacggtc gaggtgctgg tcgccgaggg
     9241 cgagggccgc aaggacgagg ccacccaccg gctctccggt cgggccgccg acaaccggct
     9301 ggtccacgtc gccgtgcccg atggggccga gcggccccgg cccggcgaca tggtcaccgt
     9361 cgaggtcacc cacgcggcgc cgcaccacct cgtcgccgac tccggcctga gcggtggcgc
     9421 tttcgccgta cggcgcaccc gtgccggtga cgcttgggag gggcggcttc gtcgtacgcc
     9481 ggtcacggca gctcggccga gcgcgcaggc ggtgctggcg gcggccgtgg tggggtctcc
     9541 accgcgatga gcgaccgcac gtcgcgctgg gagatccctg gcgacgaccc gagctcgcga
     9601 cgccggccgg cgcgcgagcc caagcggcgc ccggtgctgc gggcggtgcc acccaccatc
     9661 gggcaggggc ccgacccggc cgcccggatg ctcgccggcg aggacgccgc cggccagatc
     9721 gtgcgggccg gccacgccga gcacgccgac gtcgagcacc tggtcgcgct ggccgacgag
     9781 gtcggcctcg acgcgctggc cgccctgtgg tcgcgctcgg agccgggcac cctgccggcg
     9841 gcgctgtggg cgctctatgc gatgcggtcg tggtgtcggg cccggccccg cgagctggcc
     9901 cggctcgtca ccgcggggcg ggcccacgcc gaggtcgcgg ccggggtggc cgggctgccc
     9961 gaggacgtcg acgaggccga catcgcggcg ctcggcgacg acgtcgtccg cggtgccgtc
    10021 tcgcgtgacc tcgccacggc gctggcccgg gctgccgcgt tcgcccgggt cgtcgcggcc
    10081 ggtcgcggcg ccagcggcga cgatgcggtg gccatgactg ccgcgcacag ccgcgccgcc
    10141 tgggccgagg cggtgatggg ctcgcggctg ctcgggaccg ccgaggcctt ggaccgggcc
    10201 gccgcccggg cgcccggcct cacctccgat gatcatgaag gtgggcggga ctcctagggc
    10261 ccgcaccctt catggtcact ggccgcgggc gctcgcctct gccggcggtt cggtagcctc
    10321 cgtgcccgtg ttcgtcgccg gcgcggtgtg tccgcatccc cccgtgctcg tgcccgagct
    10381 ggccggcggg gcggtgggcg agctcgacga gctgcgccgg gcctgcgggc acgcggtgga
    10441 cgcgatgctg gcggcccgcc cggacctggt cgtggtcgtc ggtcgaagcg acgacgacca
    10501 cggtgacgag atccgtgggc tgcctgcggg ttcgtggggc gcgctgacgc cgtacggcgt
    10561 gccggtgcac gtccgcccgc cgggcaccga ccgagaccgt gcgcccgagc tgccgctgtc
    10621 gctgaccatc ggcgcctggt tgctcggccg ggcgggctgg gccgggccga ccgcgtggat
    10681 caccaccggc cccgagccgt cccgccctcg ggtcgaggcg tgcgggcggg agctggccgc
    10741 gcgggcgggc cgggtggcgg tgctggccat gggggcgggc tcggcccgtc ggctggcgac
    10801 cgggccgggc gggctggacc cggcggcccc ggcctacgac gagctggtca ccacggcgct
    10861 cggagcggcc gacaccgcga cgctgctcgg gctcgactcg gccgaggggg cccggctgct
    10921 ggtcaccggg ctcggcccgt ggcaggtgct ggccgccgcg gccgaagcgg gcgcgcaggc
    10981 gccaagcgcc cagctgctcc acgccgccac gccctatggc gtgacctatc tggtggccag
    11041 ctgggtcacc ggctgagagc cgccccggct aacgcggctg gctcgggcga tctggattgt
    11101 cgcgggagag cttgtcggcc gcctcgcgcg ccttctgctg ggccgcatcg atcttgtcgg
    11161 agtgcttgcc acccgtcttg tcgtcgacca cgtcgccagc cttctcgatc gcgtcgtcga
    11221 tcttctcgcc gtgctcgcgg gccagcttgg ccgccttgtc cttcatctcg tcgaacatgc
    11281 ccatgggaac ctccgggtcg gccggttgat gggacgtctc cttcctacct gccgcgcccg
    11341 gtgacgtccg cggaacggct gggaggatgc gcacgtgcag acgccgccgc ccccgatcgt
    11401 cgcggtggtg ggaccgaccg cggccggcaa gtctgacctc gcggtcgacc tggcgctgcg
    11461 gctcggcggg gaggtggtca acgccgactc gatgcaggtc taccgcggca tggacatcgg
    11521 caccgccaag ctcactccga ccgaacagcg cggggtcgcc caccacctgc tcgacctgtg
    11581 ggacgtgcgg gagccggcga gcgtggccgt ctaccagcgg ctggcccgcg cctgcgcggc
    11641 cgagctgggt gcggcgggca tccggcccgt gctcgccggc gggtccgggc tctacgtgcg
    11701 cgcggtgctc gacgacctcg acttccccgg caccgacccg gcggtgcggg cccggctgga
    11761 ggccgagctg gccgaggccg gccccgcccc gctgcacgag cggctggctc ggctcgaccc
    11821 cctcgcggcc gctcggatcc tgcccagcaa cgggcggcgg atcgtgcgcg ccctcgaggt
    11881 cgtgcaggcc accggccggc cgttctcggc caccctgccg gtgccgcgct acgtcgtacc
    11941 cgccgtgcag atcggcctcg aggtgccgcg tgaggccctc gacgcacgga tcgagcggcg
    12001 ggtcgaccgc atgtgggagc agggcctggt cgaggaggta cgacggctcg cggccgccgg
    12061 cctgcgtgag gggcgcaccg cgtcccgggc cctgggctac gcgcaggtgc tgcgccacct
    12121 gtcgggggag tggacggagc agcaggcccg ggacgagacg gtgcgcgcca cccgccggtt
    12181 cgcccgccgc cagcagtcct ggtttcgccg cgacccgcgg gtgcactggc tgccttacga
    12241 cgcgcccgac ctgctcgacc gggcgctcgc gctgctcgcc ccggacaggt gatcagcggg
    12301 tggccgaggg ggtggtgcgc ttggtcaggc tccaggcgag ccaccccagc gcacccagcg
    12361 gcacctcgag cacgtgcacg tagaacgcga acagcagcac gcccgcggtc gccggggcgg
    12421 ccggcacccc gagcgcgacc agcagggcgg ccgcgccggt ctccgagatg cccagcccgc
    12481 ccggggtgac gacgaccgag gtcagcaggc ggcccagcgt gtaggccgcg aagacctgcg
    12541 gcagcgtcgg gtcagcgccg acggcgcgca ggcacatcca gaacagcacg gcgtagacgg
    12601 ccacgaacgc cgtcatcccg agcgtcatcc gcacccaggc ggcgcgcacg aggtcggtgg
    12661 tccggtgccg cagcgcgatc accgccgagt cccagtgcag gtgccggtgc gagcgcacca
    12721 gccgcagcgc cagctcgccg acccgggccg cgccgcgccc gacggccagc gcggcccgtt
    12781 cgctggccag cacggtggtc gcgaccgcga cgatgagcag cagtacggcg accgcgacgc
    12841 cggccgccac cgccagccgg cgggtgacga tctcgccgcc gagcagcagc gccgcgagcc
    12901 cgagggccgg catgaccagc ttggccagcg tgttccagac cccggacacc acggccgaga
    12961 gcacgatcgc cgaccgggag aacccccacg agcggcacat cccgtaggtg atcccgacgc
    13021 cgaccgcccc gccgaacggc agcaggttgg acaccgagct gcccgccacg ttgacgatca
    13081 acgccttgta gtgcgagagc ccgggcagcg acgcgctgag cacgaacgtg tagcaccaca
    13141 tggccagcag cagcagggcg aacatgccca gcccggcggc caggctcacc cgctcgagcg
    13201 cctcggcggt ctccggccac gacgagccag tgaccttggg cagcgcccag acgatcagcg
    13261 ccaccgcgac cccggccccg accagcgtct gcagtccgcc gcgccagctc agccagccac
    13321 ggcgcgggac ctcgacctcg gtctcggtgc cgaacggggt ctcggcgggc tcgctcatcc
    13381 cggcctcgga cctcggcgtc gggaggtgcc gaccgcggcg aacgggtcgc gcagcagccg
    13441 ggacccggag ggcaccgccg ggcgcacctg ggtgaaccac tcccggccga gcacccaccg
    13501 gaagaccggc tgcggcagcc gcaggagggc cgccagcgtg cggtcacccc cgtcggcggt
    13561 ggcctgggag gcgtgcgcgg ccatcgaggc ccgcttggcc cgggcatagc ggcgtacgtc
    13621 gacccggtgg gtgatcgccg tccgcgggac gtaggcctgc tggaacgacg cgaagtcgaa
    13681 ctcgggcggg aaccggtgga ccttggaggc cagcgccagc ccgcgcagca gcaggtcgcg
    13741 gtcgacggtc gcctcgagca gcgccggggt cttggccagc gcggccgcgc gcaggccgac
    13801 ccggtgcacc tggacgtggt cggggtgccc gtagccgccg ttggcgtcgt aggacgtgag
    13861 cacgtcggcc tgctcctcgc agaggatctc ggccaaccgg gcggcggctt cctcgacgtc
    13921 ggcgtgggcg aacagcgtgc gcccggcgaa cgcctccgag accacgggcc gctcgacgtc
    13981 gggcgccagc cccgagtcgc cgtagccgag cacctcgacc cgggccacgc cgagggccgc
    14041 cgccgaccgg tgcagctcac gcagccggcg ctggcccagc cggccgtcga ccacgaggtc
    14101 gctggaggcc agcccggcct cgccggcggt ggccacgacg agcaccaccc ggtggcccgc
    14161 ggccgcggcc cgtgccatgg tgccggcggt cagcagggac tcgtcgtccg ggtgggcgtg
    14221 gaagacgacc atggtgtgcg gcacgacgtc tcatcctgcc gcatgcccgc ccgcgtcgag
    14281 ccgccgcttc gctacggtgg gggcgccgtg aagatcgcgc atctgaccga ctgctacctg
    14341 ccccgcctcg gcggcatcga ggtacaggtc cacgacgtcg ccacccgcca gctcgccgcc
    14401 gggcacacgg tcgaggtgct caccgccacc cagggcgaga cccgggtgcg cggccggtcc
    14461 cgcgacctgg tcgacggggt gccggtgcac cggctgtcga tgcgcgtgcc gttcgagctg
    14521 ccggtcaacc cgtgggccgc cgcggcggtg cgcaccgtcc tggccgaggg cggcttcgac
    14581 gtcgcccacg tgcacgccgg ggtcatctcc ccgttcgccg tcgacgcggc tcgggtctcg
    14641 gtcgggctgg gcgtgccgac gctggtgacc tggcactgcc tgctcggcgg gctcgagccg
    14701 gtgttccggc tgctcgagcg cggcaccggg tgggcgcgtc ggccgatggc gctctcggcg
    14761 gtcagcgacg tggccgcggc cccgctgcgg cgcatcgccg gacccggcgc ggaggtcgcg
    14821 gtgctgccca acggcatcga cgtcgagacc tggcgggtgg tccccgccgc ccgcgccgag
    14881 cagaccgttc acgtggtggc cacgatgcgg ctggccccgc gcaagcggcc gatcccgctg
    14941 ctgcggatcc tgcgcgacgt acgacgcctg gtgcccgacg acatccggct gcgggccacg
    15001 atcgtcggtg acggcccggc gctggcgccg gcgcgccgct acgtcgaccg gcacggcctg
    15061 ggctcctggg tccgcctgct cggccggctg cgccggtcgc agatccgtga gctttaccgc
    15121 gacgccgacg tgttcgtggc cccggccgag ctcgagtcct tcggcatcgc cgcgctcgag
    15181 gcccgctgcg ccgggctgcc ggtcgtcgcc aaggcggtca gcgggatccg cgagttcgtc
    15241 cacgacgggc aggaggggct gctggccggc agcgacctcg agatggccga tgccctggtg
    15301 cgcctggtca ccgacgccga gctgcgcgcg cgcatcgccg cgcacaaccg ggcggtcgag
    15361 cccgacatgt cctggccccg cgtcctcaag cgcaccgagg aggaatacct gcgggccatc
    15421 gcgatcatgg ctttccatcg ggacaggggc agggcctagg ctcgacgggt gatctccgac
    15481 ggcgacggcg tggtgttcgt caagggccat ggcacgcaga acgacttcct cgtgctgccc
    15541 gaccccgacg gcacgctggc cctgtctgcg cggctggtca gcgcgctgtg cgaccgccgc
    15601 cgcggcctgg gtgccgacgg ggtgctgcgg gtggtgcggg tcgaggcggc cgggcccgac
    15661 gcggccgacg cggtcggggc ggccgagtgg ttcatggact accgcaacgc cgacgggtca
    15721 ctggccgaga tgtgcggcaa cggcgtccgg gtctacgcgc ggtacctggt cgagtccggg
    15781 ctggccagcg gcccgcggct gccgatcctc acccgggccg gactggtggt ggccgagatc
    15841 gggcgcgaga ccatcgcggt ccagatgcc
//
LOCUS       DATIBB010000134         6160 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_79301, whole genome shotgun sequence.
ACCESSION   DATIBB010000134 DATIBB010000000
VERSION     DATIBB010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 6160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6160
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_79301"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..719
                     /gene="alr"
                     /locus_tag="VLC50_05180"
     CDS             <1..719
                     /gene="alr"
                     /locus_tag="VLC50_05180"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005313445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HSF26894.1"
                     /translation="DWPDLVTAALKAEAAGHVRVAGLWSHLACADEPGHPSIARQLAA
                     FTDAVEHAVRAGATPEVRHLANSAAALTLPDARYDLVRVGLATYGLSPAPAVGPAAHF
                     GLHPAMTLRARLALVKRVPAGHGVSYGHRYVTEAPTTLGLVPLGYADGIPRAASNVGP
                     LLVAGARRRVAGTVAMDQLVVDLGDADAAAGDEVVLFGPGTAGEPTAADWAEATGTIS
                     YEIVTRIGARVPRTYVGERP"
     gene            716..1795
                     /locus_tag="VLC50_05185"
     CDS             716..1795
                     /locus_tag="VLC50_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020392645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HSF26895.1"
                     /translation="MSRAGRWRRGAGVLGAVAGAAGTVAVGAALGALAERRWASSRAD
                     RVETSPEAYGSLRGRVHPVTADDGTALHVEVDGPDDAPYTVVFCHGYALRADSWHFQR
                     RDLTDLGRRVFWDQRGHGRSARGPAEHATIHQLGHDLAAVIAATAPTGPLVLVGHSMG
                     AMTIMALARHHPELFADRVSGVALIATAAGPVPGVSLGVGHLAGRLVHQLAPGLLSAL
                     TRAPRPIGGVWRVGSDFAHAVTAHWAFGSPVPASLVRFATEMITSTPLEVIADFYPAF
                     DVLDEMDSLEVLNQGVETLVLVGDHDQLTPLEHSSAIVAAVHGAELVVVPHAGHLVML
                     EHPAVVTAHLRGLVHRATRALPRSA"
     gene            1792..2457
                     /locus_tag="VLC50_05190"
     CDS             1792..2457
                     /locus_tag="VLC50_05190"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018347820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HSF26896.1"
                     /translation="MSRRPTSTASTDVDAVARRHIAAAARAQDWTELAHAIEGCAACP
                     DLVAGRSHVVVGAFPAGARLLLVGEAPGAAEDAAGLPFVGRSGALLDELLAEAGLGRD
                     DVAITNVVRCRPPGNRTPRRAEVRRCRPWLLRQLELVDPLLVVTLGVTAAAAFFGPGA
                     RIGTLRGAVHLVAGRSVLATYHPSAALRFGPAGQPIQSLRQDLALAATVLRQTAPREE
                     SNR"
     gene            2454..3401
                     /gene="tsaE"
                     /locus_tag="VLC50_05195"
     CDS             2454..3401
                     /gene="tsaE"
                     /locus_tag="VLC50_05195"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HSF26897.1"
                     /translation="MSAAPPSGLVVRDACPSDAQVIRDVVVEAFLDRPRLDPPTTATD
                     ETVHSVREALESYGGVVAELEGQVVGALLLAREADVLTLRRVATRPGYRRRGVAGAMA
                     AHAEARAQRIGVRVVALSARPELPATVGFWRNRGFTQTGRRGHLLDLAKPAPVSIVLP
                     DAAATRRLGARIAGLLHAGDLVVLAGELGAGKTTLAQGLGAALGVRGEVTSPTYVIAR
                     VHPSTSGGPDLVHVDAYRLGGQLEVDDLDLDASVEESVTVVEWGEGKVEALAEDRLEV
                     RIDRDAPGGRLAVVRGIGRRWLDAVDGLTELASIAWPTC"
     gene            3395..4099
                     /gene="tsaB"
                     /locus_tag="VLC50_05200"
     CDS             3395..4099
                     /gene="tsaB"
                     /locus_tag="VLC50_05200"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014136240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HSF26898.1"
                     /translation="MLTRPTLALDTSTPALTVAVVHAPGGAGEAGAPVVLAAREVVDA
                     RRHAELLTPLIETTLVAAGVEAAALTRLVVGVGPGPFTGLRIGLMTASALGDALGVPV
                     EGVCSLDALAVEARDAGIEGPLLVATDARRREIYWATYDETGRSAGPAVGRPADVLAE
                     LAATGWTGAVVGDGARLHAAVFAEAGIDVAAPWYPRAGDLVTALHRGAELLAPRPLYL
                     RRPDAQVPGAPKPVLA"
     gene            4096..4617
                     /gene="rimI"
                     /locus_tag="VLC50_05205"
     CDS             4096..4617
                     /gene="rimI"
                     /locus_tag="VLC50_05205"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015610776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HSF26899.1"
                     /translation="MSAATRSPAPTLRRMRWWDVEPVVELERELFADDPWSAEAFWSE
                     LAGVPATRHYVVATDAGQAPDGDVERVVGYAGLMAVPGQADVQTIAVAAAAQGRGLGA
                     RLLEELLAEAERRDEPAVLLEVRADNAAAIALYERHGFARLARRRGYYQPSGADALVM
                     RRLRRTTTEEASR"
     gene            4623..5669
                     /gene="tsaD"
                     /locus_tag="VLC50_05210"
     CDS             4623..5669
                     /gene="tsaD"
                     /locus_tag="VLC50_05210"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012854571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HSF26900.1"
                     /translation="MRDEPVVLGIETSCDETGIGIVRGTTLLADAVASSVEQHARFGG
                     VVPEVASRAHLEAMVPMVHQALSEAGMALRDVDALAVTSGPGLAGALMVGVAAAKAYA
                     LAAGKPLYGVNHLAAHVAVDQLEHGSLPEPCVALLVSGGHSSLLLVPDVTADVRPLGQ
                     TLDDAAGEAFDKVARVLGLGFPGGPYVDRAAREGDGAAIAFPRGLTAARDLREHPYDF
                     SFSGLKTAVARWVEARRRDGADVPVADVAASFQEAVVDVLTRKAVAACREYGVRDLVL
                     AGGVAANSRLRVLAEARCVEAGLRLRVPRPKLCTDNGAMVAALGAELVARDRTPSALD
                     LPADSSLPVTTVVA"
     gene            complement(5929..>6160)
                     /locus_tag="VLC50_05215"
     CDS             complement(5929..>6160)
                     /locus_tag="VLC50_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013830119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF26901.1"
                     /translation="VSAPTMVVGIDSDRLYPVHQQAEIASLVPGAAPLQVVASDHGHD
                     GFLTETAMVGSLVRGFLEDVHGRDTGSARRTA"
BASE COUNT          683 a         2341 c         2326 g          810 t
ORIGIN      
        1 ccgactggcc cgacctggtc accgccgcgc tcaaggccga agcggccggg cacgtgcggg
       61 tggccgggct gtggtcgcac ctggcctgcg ccgacgagcc gggccacccg tccatcgcgc
      121 gccagctcgc cgcgttcacc gacgcggtcg agcacgccgt ccgggccggc gccaccccgg
      181 aggtccgcca cctggccaac tcggcggccg cgttgaccct gccggacgcc cgctacgacc
      241 tcgtgcgggt cgggctggcc acctacggcc tctcgccggc gccggcggtc ggcccggcgg
      301 cgcacttcgg gctgcacccg gcgatgacgc tgcgcgcccg gctggccctg gtcaagcggg
      361 tcccggccgg gcacggggtc tcctacggcc accgctacgt caccgaggcg cccaccacgc
      421 tcgggctggt cccgctcggc tacgccgacg ggatcccccg cgcggccagc aacgtcgggc
      481 cgctgctcgt tgcgggcgct cgccgacgcg tcgccggcac ggtggccatg gaccagctcg
      541 tggtcgacct cggcgacgcc gacgccgcgg caggcgacga ggtcgtgctg ttcgggcccg
      601 gcaccgcggg cgaacccacc gccgccgact gggccgaggc gaccggcacg atctcctacg
      661 agatcgtcac ccggatcggt gcccgggtgc cgcggaccta cgtcggagag cgcccgtgag
      721 ccgtgccggc cggtggcggc gcggggcagg ggtgctcggc gcggtggcgg gcgccgcggg
      781 cacggtagcc gtcggtgccg cactgggcgc gctcgccgag cggcgctggg cctcctcgcg
      841 cgccgaccgg gtcgagacct cacccgaggc ctacggctcg ctgcgcgggc gggtgcaccc
      901 ggtcaccgcc gacgacggca ccgcgctgca cgtcgaggtc gacggccccg acgacgcgcc
      961 ctacaccgtg gtgttctgcc acggctacgc gttgcgcgcc gactcgtggc acttccagcg
     1021 ccgcgacctg accgaccttg ggcggcgggt gttctgggac cagcgcggcc acggccgctc
     1081 ggcgcgcggc ccggccgagc acgcgaccat ccaccagctc ggccacgacc tcgccgcggt
     1141 gatcgcggcc accgcgccga ccggcccgct cgtgctcgtc ggacactcga tgggggccat
     1201 gacgatcatg gcgctggccc gtcatcatcc ggagctgttc gccgaccggg tcagcggggt
     1261 ggcgctcatc gcgacggcgg ccggcccggt ccccggcgtc tcgctgggcg tcgggcacct
     1321 ggccgggcgg ctcgtgcacc agctggctcc cggcctgctc tcggcgctca cccgagcccc
     1381 tcgcccgatc ggcggcgtct ggcgggtcgg cagcgacttc gcccacgcgg tgaccgccca
     1441 ctgggcgttc ggctcgccgg tgcccgcctc gctggtgcgc ttcgccaccg agatgatcac
     1501 ctcgacgccg ctcgaggtga tcgccgactt ctaccccgcc ttcgatgtgc tcgacgagat
     1561 ggactcgctc gaggtgctca accaaggtgt cgagacgctc gtgctggtcg gtgaccacga
     1621 ccagctgacc ccgctcgagc acagcagcgc gatcgtcgcg gccgtgcacg gcgccgagct
     1681 ggtcgtcgta ccgcatgccg ggcacctcgt gatgctcgag caccccgccg tggtgaccgc
     1741 ccacctgcgc ggcctcgtcc accgggccac ccgagccctg ccgagatcgg catgagccgg
     1801 cgcccgacca gcacggcgag caccgacgtc gacgccgtcg cccgccggca catcgcggcg
     1861 gcggctcggg cgcaggactg gaccgagctc gcgcacgcga tcgagggctg cgccgcgtgc
     1921 cccgacctgg tcgccggccg cagccacgtg gtggtcggtg ccttcccggc cggcgcacgg
     1981 ctcctgctcg tcggcgaggc gcccggagcg gccgaggacg ccgccggcct gccgttcgtc
     2041 ggccggtccg gagcgctgct cgacgagctg ctggccgagg ccgggctggg ccgagacgat
     2101 gtggccatca ccaacgtcgt caggtgccgc ccgcccggca accggacccc gcggcgggcc
     2161 gaggtgcgcc ggtgccggcc ctggctgctg cgccagctcg agctcgtcga cccgctgctg
     2221 gtcgtcacgc tcggggtcac cgcggccgcc gcgttcttcg ggccgggcgc ccgcatcggc
     2281 acgctgcgcg gcgcggtgca cctggtcgcc ggccgctcgg tgctggccac ctatcatcca
     2341 tcggcggcgt tgcggttcgg cccggcgggc cagccgatcc aatcgctgcg ccaggacctc
     2401 gccctggccg cgacggtgct gcgccagacg gcaccgcggg aggagagcaa ccggtgagcg
     2461 cagccccgcc aagtgggctc gtcgtccgcg acgcctgccc gtccgacgcc caggtcatcc
     2521 gtgacgtggt cgtcgaggcc ttcctcgacc ggcctcggct cgatccgccc acgacggcga
     2581 ccgacgagac cgtgcacagc gtccgagagg cgctcgagtc ctacggcgga gtggtcgccg
     2641 agctcgaagg ccaggtggtc ggcgcgctgc tgctggcccg ggaggccgac gtgctcacgc
     2701 tgcggcgggt ggccacccgc cccggctacc gccgccgcgg cgtggccggc gcgatggccg
     2761 cccacgccga ggcgcgagcc cagcgcatcg gcgtacgtgt cgtcgccctc tcggcgcgcc
     2821 ccgagctgcc ggccacggtg gggttctggc gcaaccgggg gttcacccag accggccgac
     2881 gcgggcacct gctcgacctg gccaagccgg cgcccgtctc catcgtgctg cccgacgcgg
     2941 cggcgacccg ccggctcggc gcccggatcg ccgggctgct gcacgccggt gacctggtcg
     3001 tgctcgccgg tgagctgggc gccggcaaga ccacgctggc ccagggcctg ggcgccgcgc
     3061 tcggcgtgcg cggggaggtc accagcccga cctacgtgat cgcccgcgtc catccctcga
     3121 cgagcggagg gcccgacctc gtgcacgtgg acgcctaccg gctcggcggc cagctcgagg
     3181 tcgacgacct cgacctcgac gcgtcggtcg aggagtcggt cacggtggtc gagtggggcg
     3241 agggcaaggt cgaggcgctg gccgaggacc ggctcgaggt gcgcatcgac cgcgacgcgc
     3301 cgggcggccg gctcgcggtg gtccgcggca tcggccgacg ctggctggac gccgtcgacg
     3361 ggctgaccga gctggcgtcg atagcctggc cgacgtgctg acgcgcccga ccctcgccct
     3421 cgacacctcg acgcccgccc tcaccgtcgc ggtggtccac gcgccgggcg gtgccggtga
     3481 ggccggcgcc ccggtggtgc tggccgcccg tgaggtggtc gatgcccgtc gacacgccga
     3541 gctgctcacc ccgctgatcg agaccacgct ggtcgcggcc ggcgtcgagg ccgccgcgct
     3601 gacccggctg gtcgtcgggg tcggtcccgg cccgttcacc gggctgcgga tcgggctgat
     3661 gaccgcgagc gcgctcggcg acgcgctcgg cgtaccggtc gagggcgtct gctcgctgga
     3721 cgcgctggcg gtcgaggccc gcgacgccgg gatcgagggg ccgctgctgg tcgcgaccga
     3781 cgcgcgccgg cgggagatct actgggcgac ctacgacgag accgggcgca gcgcgggccc
     3841 ggcggtcggg cgcccggccg acgtccttgc cgagctggcc gccaccggct ggaccggcgc
     3901 cgtcgtgggc gacggcgcgc ggctgcacgc ggccgtcttc gccgaggccg gcatcgacgt
     3961 cgcggcgccg tggtacccgc gcgccggtga cctcgtcacc gccctgcacc ggggcgccga
     4021 gctgctggcg ccgcggccgc tctacctgcg ccggcccgac gcccaggtgc ccggcgcgcc
     4081 caagccggtg ctcgcgtgag cgccgccacg cgctcgcccg cgccgacgct gcgtcggatg
     4141 cggtggtggg acgtcgagcc ggtggtcgag ctcgagcgcg agctgttcgc cgacgacccg
     4201 tggagcgccg aggccttctg gtccgagctg gccggggtgc cggccacccg ccactacgtc
     4261 gtcgccaccg acgccgggca ggcccctgac ggtgatgtgg aaagggtcgt cggctacgcc
     4321 gggctgatgg cggtgcccgg gcaggccgac gtgcagacca tcgcggtggc cgcggctgct
     4381 cagggtcggg ggctgggggc tcggctgctc gaggagctgc tggccgaggc cgagcgccgt
     4441 gacgagcccg ccgtgctgct cgaggtccgc gccgacaacg cggccgccat cgcgctctac
     4501 gagcggcacg gcttcgcgcg gctggcccgc cggcgcggct actaccagcc ctccggcgcc
     4561 gatgcactcg tgatgcgccg actgcgccgt acgacgactg aggaggcgag ccggtgaccg
     4621 cgctgcgcga cgaaccggtg gtgctgggca tcgagacctc gtgcgacgag accgggatcg
     4681 ggatcgtgcg gggcaccacg ctgctcgccg acgcggtggc ctccagcgtc gagcagcacg
     4741 cccggttcgg cggggtggtg cccgaggtgg ccagccgggc gcacctggag gcgatggtgc
     4801 cgatggtgca ccaggcgctg tccgaggcgg ggatggccct gcgcgacgtc gacgcgctcg
     4861 ccgtgaccag cgggcccggt ctcgccggcg cgctcatggt cggggtcgcc gcggccaagg
     4921 cctacgcgct ggccgccggc aagccgctct acggcgtcaa ccacctggcc gcccacgtgg
     4981 ccgtcgacca gctcgagcac gggtcgcttc cggagccgtg cgtggcgctg ctggtttccg
     5041 gcgggcactc gtcgctgctg ctcgtgcccg acgtcaccgc cgacgtccgc ccgctgggcc
     5101 agaccctcga cgacgcggcg ggggaggcct tcgacaaggt ggcccgggtg ctgggcctgg
     5161 gctttcccgg cggcccctac gtcgaccggg ccgcgcgtga gggtgacggc gcggcgatcg
     5221 cgttccctcg cggactgacc gccgcgcgcg acctgcgcga gcacccctac gacttctcgt
     5281 tctccgggct caagaccgcg gtggcccggt gggtcgaggc gcgccggcgc gacggcgccg
     5341 acgtgccggt cgccgacgtg gccgcgtcgt tccaggaggc cgtcgtcgac gtgctgaccc
     5401 gtaaggcggt ggccgcctgc cgcgaatacg gggtgcgcga cctcgtcctc gccggcggcg
     5461 tggcggccaa ctcgcggctg cgggtgctcg ccgaggcgcg ctgcgtcgaa gccgggctgc
     5521 ggctacgggt gccgcgaccc aagctgtgca ccgacaacgg cgccatggtc gccgcgctgg
     5581 gcgccgagct ggtggcccgt gaccgtaccc cgtcggcgct cgacctgccc gccgactcgt
     5641 cgctgccagt caccaccgtc gtcgcctgag cgcccgccgc ccccggcgtg atcatgaagg
     5701 ttgaccgccc ttaggagacg gggtaggcct tcatgatcaa gcagaattcg tggccggggc
     5761 tcggtttcgc gatgttgtgt caattcgtgc gggtcctggg gctgcgatct tccacaatct
     5821 ttggccgcgt gcaggagtcg cttgatcacc gatgcgatgc gggcacggat tgcttgatca
     5881 ccggcgcgag gtgggcaccg attgcttgat caccacgagg gggtggaatc aggcggtacg
     5941 acgggccgag ccggtgtcgc gcccgtgcac gtcctcgagg aacccgcgca cgagtgagcc
     6001 gaccatcgcc gtctcggtga ggaagccgtc gtggccgtgg tcgctggcca ccacctgcag
     6061 cggggcggcg cccggcacca gcgaggcgat ctcggcctgc tggtgcaccg ggtagagccg
     6121 gtcggagtcg attccgacca ccatcgtcgg tgcgctgacc
//
LOCUS       DATIBB010000135         5071 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_1790121, whole genome shotgun sequence.
ACCESSION   DATIBB010000135 DATIBB010000000
VERSION     DATIBB010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 5071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5071
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1790121"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..490)
                     /locus_tag="VLC50_05220"
     CDS             complement(<1..490)
                     /locus_tag="VLC50_05220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF26902.1"
                     /translation="MFDNTGDGPTDDAIGSQPTDTSHTDTSHTEPLAPADPLAPADPL
                     LPVDPPQPAVSTPEPPALPGLAGRLLAGLDPASAAAVRAAVSHEQVPVDDDVSLPGWA
                     SQPAGPDLAVAIAELDPADLDEPARVELIAAYERLAGWVHASQVVALAGFAAIRPILD
                     PDP"
     gene            complement(573..1607)
                     /locus_tag="VLC50_05225"
     CDS             complement(573..1607)
                     /locus_tag="VLC50_05225"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011754149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0009168 - purine ribonucleoside
                     monophosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HSF26903.1"
                     /translation="MRPLDTLPKAHLHLHFTGSMRHGTLVELAAEHGVRLPEALVSAW
                     PPRLRGTDERGWFRFQRLYDIARSVLRRESDVRRLVLEAAQDEATEGSGWLELQVDPS
                     GYAARFDGLSAFTELVLDAARDATARTGVGIGIVIAANRTRHPLDARTLARLAAQYVD
                     RGVVGFGLSNDERRGKAEDFAAAFAIARRAGLASLPHGGELAGADSVRACLETLAADR
                     VGHGVRAVEDPALLDRLAERGVACEVCPASNVALGVWPSPEKVPLPTLLAAGVTVALG
                     ADDPLLFGPRLLAQYAMAREVHGLDDVALASLARSSVQASCAPSEVRGRLLGGIDAWL
                     ATAPPTRPDR"
     gene            complement(1647..2885)
                     /locus_tag="VLC50_05230"
     CDS             complement(1647..2885)
                     /locus_tag="VLC50_05230"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013117898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HSF26904.1"
                     /translation="MTGTTTTAPTTAASRRVSTRIGAISESATLAVDAKAKAMKAAGR
                     PVIGFGAGEPNFPTPPYVVEAAAAACVDPRNHRYSPAAGLPELRAAIAAVTLRDNGVT
                     VAPEQVLVTNGGKQAVYETMATLLDPGDELLLPAPYWTTYPEAIRLAGATPVPVFAGP
                     EQDYLVTVDQLEAARTPRTKALLLCSPSNPTGAVYAPEAIRAIGEWALEHGLWVISDE
                     IYQHLTYDGMPFTSVLAAVPDLAERTVLLNGVAKTYAMTGWRVGWMIGPRDVVKAATN
                     LQSHLTSNVANVSQRAALAALEGGLDVVAEMRAAFDRRRRTIVERLSAIPGITCPTPK
                     GAFYAYPNVEGLLGRSIGGRTPTTSAELAELILEQVEVAVVPGEAFGPSGYLRFSYAL
                     ADEDLVEGVSRIARLVESAS"
     gene            3063..3135
                     /locus_tag="VLC50_05235"
     tRNA            3063..3135
                     /locus_tag="VLC50_05235"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3096..3098,aa:Trp,seq:cca)
     gene            3270..3533
                     /gene="secE"
                     /locus_tag="VLC50_05240"
     CDS             3270..3533
                     /gene="secE"
                     /locus_tag="VLC50_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020575508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HSF26905.1"
                     /translation="MSQTRGSTATPAHGPSGRAGDEDQRSLFARLSLYYRQIVAELRK
                     VIWPTRKELINYTAVVIVFVLIVIAYVSVLDVIFGRVVLAIFG"
     gene            3568..4398
                     /gene="nusG"
                     /locus_tag="VLC50_05245"
     CDS             3568..4398
                     /gene="nusG"
                     /locus_tag="VLC50_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014054145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HSF26906.1"
                     /translation="MRPPVHKKEEVVTVPESPQSEQTPQSPEQPEVLAPADGADTSDA
                     VATDVVDTVDDADVAAPADDEAAPADDEAASADVDEAEPADVDPVAEFREAMRHSPGD
                     WYVVHSYAGYENRVKSNLESRATSLNMEEFIFQVEVPQEDAIEIKNGQRKQVRRNKFP
                     GYVLVRMDLTDESWGAVRNTPGVTGFVGHGHQPSPLTLDEVVKILAPEVGETKAPVEV
                     KVLDFAVGDSVTVIDGPFATLQMTISEITAESQKIKGLVEIFGRETPVELSFNQIQKN
                     "
     gene            4418..4849
                     /gene="rplK"
                     /locus_tag="VLC50_05250"
     CDS             4418..4849
                     /gene="rplK"
                     /locus_tag="VLC50_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010058817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HSF26907.1"
                     /translation="MPPKKKLAAIIKLQINAGAATPAPPVGPALGQHGVNIMEFCKAY
                     NAATESQRGQVIPVEISVYEDRSFTFVTKTPPAAKLILKAAGVEKGSGEPHRTKVATI
                     TRDQVREIAQTKMPDLNANDLDAAEKIIAGTARQMGIDVKG"
     gene            4953..>5071
                     /locus_tag="VLC50_05255"
     CDS             4953..>5071
                     /locus_tag="VLC50_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011293080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HSF26908.1"
                     /translation="MKRSKAYRAAAEKIDRERLYAPDEAVRLVKETATTKYDAT"
BASE COUNT          785 a         1831 c         1786 g          669 t
ORIGIN      
        1 ctgggtcggg gtcgaggatt gggcggatgg ccgcgaagcc ggccagcgcg acgacctggc
       61 tggcgtggac ccagccggcg aggcgttcgt aggcggcgat cagctccacc cgggccggct
      121 cgtcgaggtc ggccggatcc agctcggcga tggccacggc caggtctggt ccggcgggct
      181 gactggccca ccctggcagg ctgacgtcgt cgtcgaccgg gacctgctcg tggctgaccg
      241 cggcgcggac cgccgccgcc gaggccggat ccagcccggc gagcagccgc cccgccaacc
      301 ccggcaacgc cggtggctcg ggcgtcgaga cagccggctg gggcgggtca accggcagga
      361 gcgggtcagc cggcgcgagc gggtcagccg gcgcgagcgg ctcggtgtgg ctggtgtcgg
      421 tgtggctggt gtcggtgggc tgcgacccga tggcgtcgtc ggtcggacca tcccccgtgt
      481 tatcgaacat gtgttcgagt ctagcggaga gctccgtcaa acggaagcat ctgcgagcaa
      541 tcaacaccta cccatcggat tcaggattgc cgtcaccggt cgggccgcgt gggcggcgcg
      601 gtcgccagcc aggcgtcgat gccgccgagc aggcgtccgc ggacctcgct cggcgcgcag
      661 gacgcctgca ccgacgagcg ggccagcgag gccagggcca cgtcgtccag accgtggacc
      721 tcgcgggcca tcgcatattg agccagcagg cgtgggccga agagcagcgg gtcatcggct
      781 cccagagcaa ccgtgacgcc ggcggccagc aaggtcggca gcggcacctt ctccgggctc
      841 ggccacacgc cgagggccac gttggaggcc gggcacacct cgcaggcgac tccgcgctcg
      901 gccagccggt cgagcagggc cgggtcctcc accgcgcgca cgccgtgccc aacccggtcg
      961 gcggcaagtg tctcgaggca tgctcggaca ctgtcggccc ccgccagctc gccgccgtgc
     1021 ggcaggctgg cgagtccggc ccggcgggcg atcgcgaacg cggctgcgaa gtcctcggcc
     1081 ttccctcgcc gctcgtcgtt ggacagcccg aagccgacca cgccgcggtc gacatactgg
     1141 gcggccagtc gggccagcgt gcgggcgtcg agcgggtggc gggtccggtt ggccgcgatc
     1201 acgatgccga tccccacgcc ggtgcgggcg gtcgcgtccc gagccgcgtc gaggaccagc
     1261 tcggtgaagg cgctcagccc gtcgaagcgg gcggcgtagc ccgacgggtc gacctgcagc
     1321 tctagccacc cggagccctc ggtcgcctcg tcctgcgcgg cctcgagcac caggcggcgt
     1381 acgtcggact cccggcgcag caccgagcgg gcgatgtcgt agaggcgctg gaagcggaac
     1441 cagccgcgct cgtcggtgcc gcgcagccga ggcggccacg ccgagaccag cgcctccggc
     1501 aggcggaccc cgtgctcggc ggccagctcc acgagcgtgc cgtggcgcat cgagccagtg
     1561 aagtgcaggt gcaggtgcgc cttgggcagc gtgtcgagcg ggcgcacgct ggtgacccta
     1621 gggccaacgg gcccctgggt cgcggatcag ctggccgact cgaccagccg ggcgatgcgg
     1681 ctcacgccct cgaccaggtc ctcgtctgcg agcgcgtagg agaagcgcag gtagccgctg
     1741 ggcccgaacg cctcacccgg caccaccgcc acctcgacct gctcgaggat caactcggcc
     1801 agctcggccg acgtcgtcgg ggtgcgcccg ccgatcgacc gcccgagcag cccctcgacg
     1861 ttggggtagg cgtagaacgc gcccttgggg gtcgggcagg tgatcccagg gatggccgag
     1921 agccgctcga cgatcgtgcg ccggcgccgg tcgaacgccg cacgcatctc ggccaccacg
     1981 tcgagcccgc cctccagcgc ggccagcgcg gcccgctgcg agacgttggc cacgttgctg
     2041 gtcaggtggg actgcaggtt ggtcgccgcc ttgaccacgt cgcgcgggcc gatcatccag
     2101 cccacccgcc agccggtcat cgcgtaggtc ttggcgacgc cgttgagcag cacggtgcgc
     2161 tcggccaggt cgggcaccgc cgccagcacc gaggtgaacg gcatgccgtc gtaggtcagg
     2221 tgctggtaga tctcatcgct gatcacccac agcccgtgct cgagggccca ctcgccgatc
     2281 gcccggatcg cctcgggcgc gtagaccgcc ccggtcgggt tgctgggcga gcacagcagc
     2341 agggccttgg tccgcggggt gcgggcggcc tcgagctggt cgacggtgac gaggtagtcc
     2401 tgctcgggcc cggcgaacac cggcaccggg gtggccccgg ccagccggat cgcctccgga
     2461 taagtcgtcc agtagggcgc cggcagcagc agctcgtcgc cggggtcgag cagcgtcgcc
     2521 atcgtctcgt agaccgcctg cttgccaccg ttggtgacca gcacctgctc cggcgccacc
     2581 gtcacgccgt tgtcacgcag ggtgaccgca gcgatggcgg cccgcagctc gggcaggccc
     2641 gcggcggggg agtagcggtg gttgcgcggg tcgacgcagg cggcggcggc ggcctctacg
     2701 acgtacggcg gtgtcgggaa gttgggctca ccggctccga aaccgatcac cggccgaccc
     2761 gcggccttca tcgcctttgc cttggcatcg acagcgagcg tggcggactc gctgatggcc
     2821 ccgatccggg tcgagacccg gcgggaagcg gcggtggtgg gcgcggtggt cgtcgtaccg
     2881 gtcatggtcc aatgctcgca ccgccgtccg ctgagccggc gaggtgtcca ccggccggac
     2941 gtcctcggcc cggcgcggtt cgacgcccgg gcgtccaagc gcctacactc cctgcgtggt
     3001 ggctcaaagc ctctgcgtca gcacgcccaa atgcgtgtgc ccgcagcggc gcgagtcccc
     3061 gaagggtcgt agctcaattg gtagagtccc ggtctccaaa accggtggtt gggggttcga
     3121 gtccctccgg ccctgcggcg cacgccgcgc gagctcgcac aggacggcag ccggtccacc
     3181 cggtggccgc cagcacggca tcagcacgac gtgacgtgca ccgcccaaca gggcggaccg
     3241 aggaacgacc gggacgaggg tgaggaccag tgagccagac ccgcgggtcc accgcgacac
     3301 ctgcgcacgg cccctccggc cgcgccggtg atgaggacca gcgcagcctg ttcgcacgcc
     3361 tgtcgctcta ctaccgacag atcgtggccg agctgcgcaa ggtcatctgg ccgacccgca
     3421 aggagctgat caactacacg gccgtggtga tcgtcttcgt gctgatcgtc atcgcctacg
     3481 tctccgtgct cgacgtgatc ttcggtcgcg tggtcctcgc gatcttcggc tagccgggtc
     3541 acgcggtcca ccgtgcaggt ggtgccggtg cgcccgcccg tccacaagaa ggaagaagtc
     3601 gtcacagtgc ccgagtcgcc ccagagcgag cagacgccgc agtcgcccga gcagccggag
     3661 gtccttgccc cggctgacgg tgccgacacg agcgacgccg tcgcgacgga cgtcgtcgac
     3721 acggtcgacg acgctgacgt ggcggccccg gccgacgacg aggccgcgcc ggctgatgac
     3781 gaggccgcgt cggctgacgt cgacgaggcc gagccggctg acgtcgaccc ggtcgccgag
     3841 tttcgtgagg cgatgcgcca ctcgcccggc gactggtacg tcgtgcactc ctacgccggc
     3901 tacgagaacc gggtcaagag caacctcgag tcccgggcga cctcgctcaa catggaggag
     3961 ttcatcttcc aggtcgaggt cccccaagag gacgcgatcg agatcaagaa cggccagcgc
     4021 aagcaggtac gccgcaacaa gttccccggc tacgtcctcg tccgcatgga cctcaccgac
     4081 gagtcctggg gcgcggtgcg caacaccccc ggcgtcaccg gtttcgtggg ccacgggcac
     4141 cagccctctc cgttgaccct tgacgaggtc gtcaagatcc tcgccccgga ggtcggcgag
     4201 accaaggcgc cggtcgaggt caaggtgctc gacttcgccg tcggcgactc cgtcaccgtc
     4261 atcgacggcc cgttcgccac cctgcagatg accatcagcg agatcaccgc cgagtcgcag
     4321 aagatcaagg gcctggtgga gatcttcggc cgcgagacgc cggtcgagct gtcgttcaac
     4381 cagatccaga agaactgacc cgtaaggacc cggacccatg ccccccaaga agaagctcgc
     4441 ggcgatcatc aagctgcaga tcaacgccgg cgcggccacg ccggccccgc cggtcggccc
     4501 cgcgctgggc cagcacggcg tcaacatcat ggagttctgc aaggcctaca acgcggccac
     4561 cgagtcgcag cgtggccagg tgatccccgt ggagatctcg gtctacgagg accgctcgtt
     4621 caccttcgtg accaagaccc cgcccgcggc caagctcatc ctcaaggccg ccggtgtcga
     4681 gaagggctcc ggcgagccgc accgcaccaa ggtcgccacg atcacccgcg accaggtccg
     4741 cgagatcgcg cagaccaaga tgcccgacct caacgccaac gacctcgacg ccgccgagaa
     4801 gatcatcgcc ggcaccgctc gccagatggg catcgatgtc aagggctgac gcccactccc
     4861 gcactcccag tggcagggcc cgcttcggtc cgtaccacga ctcccgcccg tacgggggaa
     4921 ctccccgcat ggcccgtcga caggagcagc acgtgaagcg cagcaaggcc taccgcgccg
     4981 cagccgagaa gatcgaccgc gagcgcctct acgccccgga cgaggcggtc cgcctcgtca
     5041 aggagacggc cacgaccaag tacgacgcca c
//
LOCUS       DATIBB010000136        10224 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_1920820, whole genome shotgun sequence.
ACCESSION   DATIBB010000136 DATIBB010000000
VERSION     DATIBB010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 10224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10224
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1920820"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2071)
                     /gene="cutA"
                     /locus_tag="VLC50_05260"
     CDS             complement(<1..2071)
                     /gene="cutA"
                     /locus_tag="VLC50_05260"
                     /EC_number="1.2.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020550887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic carbon-monoxide dehydrogenase large
                     subunit"
                     /protein_id="HSF26909.1"
                     /translation="MTTKMFGAPVQRREDLRLVVGKGRYVDDLGQGALEAAFLRSPHA
                     HARIVDIDVTGALDVDGVVAVYTYEDLEGRVAEPLPLLIPHPSLHAPRTAYALAPEVV
                     NHVGEAIVMVVARDRYVAEDAVGRIVVTYDPLPPVVGIATARAAVHAVHEDIPDNVAA
                     HLVQDVGDVEAALRKAPHVLELDLDFSRSASTPMEGKGVYARWDAEDSSLRVYSSTQA
                     STSVRAAIAAKLELPLTKVECIAPDVGGGFGVKIMHPWPEEILVSWAARRLAAYEPRG
                     VKWTEDRREHFVSAAHERGQLQHVTVGFDDEGRLLGLDVKVWHDHGAYTPYGIIVPII
                     TSTQLLGPYKPEAYRCQVWSLYTNTVIVTPYRGAGRPQGVFAMERTMDAIADYLGIDR
                     TVVRARNFIQPDEMPYDHGLIFQDGRPLIYDSGDFPASLEKLTKLIGWDDFTAYREAA
                     RAEGRQVGIGLACYVEGTGVGPYEGGHVQVETSGKVKVATGLTSQGQGHQTVFAQIVA
                     DELGVPIEDIEVVTGDTRRFGYAVGTFASRAAVMSGNAIALAARNVRAKALRIAGEAL
                     EADPGDLEIVEGVVRVKGSPDSSIPLATVAVLSNPLRYAFDDAAKAATQFVVGDISKP
                     PVADDDEPGLEGKDYYSPTQSTFANGMHAVIVETDPGTAETKILRYCVVHDCGPLINP
                     MIVEGQVH"
     gene            complement(2068..2553)
                     /locus_tag="VLC50_05265"
     CDS             complement(2068..2553)
                     /locus_tag="VLC50_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005518924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HSF26910.1"
                     /translation="MAVRLRVNGVLHEVTVPVRRLLSDCLRHDLGLTGTHVGCEHGVC
                     GACTVLLDGTPVRSCLLLAVTVDGHDVTTIEGLADPDGTLHPVQQAFRECHGLQCGFC
                     TPGFLTTIAAFLEDNPAPTEAAAREAVSGNLCRCTGYQNIVASVLRAAQIRREREEGA
                     R"
     gene            complement(2598..3485)
                     /locus_tag="VLC50_05270"
     CDS             complement(2598..3485)
                     /locus_tag="VLC50_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014738255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HSF26911.1"
                     /translation="MKPSPFTYTAPRSLEDAVIALAEAGHDGKVLAGGQSLLPLLSMR
                     LAAPGRLIDINRLDELAYVRVAPDAVHVGALARHAEVERHEAAYEAQPLLRQALRQVA
                     HPTIRNRGTTVGSIAHADPSGEMTAVLTLLGGTVEAVSSHGRRDIAAADFFVGPLETS
                     LQVGEMVQAVRFPRRRAATGTSFLESARRHGDYALVGVASLVELDDDLRIRAARAAYI
                     SVAPTPALLDLTEALAGATYDAGDWAAAGALAAARTEPEPDIHASADYRSRLVEVLTA
                     RGLAEAARHAAGLSTEGAL"
     gene            complement(3561..5036)
                     /locus_tag="VLC50_05275"
     CDS             complement(3561..5036)
                     /locus_tag="VLC50_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HSF26912.1"
                     /translation="MAIGWKLHGDGSIAGPDDVVRPDERLSWGKTVGLGAQHVVAMFG
                     ATFVFPIIMGLNPQLAILMSGIATICFLLIVQGKVPSYLGTSAAFVGGVAAIRAQEGS
                     SANVTGAILIAGVVLALVGVIIHFAGARVIYAVLPPVVTGAVVMLIGFNLAPVVANIY
                     WPQDQWIALIVMAAVIGMTVGFRGFFGRIAIFLGLVLGYALSWIFDRAFGQITSFDAG
                     AGEVTTHFRVNWSGVADAAWFGFPQKTMLADGKESIVGWHTPDIKWTFVLLVLPAVIA
                     LIAENTGHVKAVAEMTNTDLDPVMGKAIAADGVGTIIASSVGGSPTTTYAENIGVMAA
                     TRVYSTAAYFVAAAVAIIFGFSPKFGAVISATPGGVLGGITVILYGMIGLLGAKIWKE
                     NQVDFGNPVNLVPVAAGIIIAIGDTSLVFTDSFSLSGIALGTIVTVLGWHVARWLAPA
                     HLAESAVAVVGTTLVEPGTHGVPDPYPEVSEQDKGGASHTS"
     gene            complement(5303..7036)
                     /locus_tag="VLC50_05280"
     CDS             complement(5303..7036)
                     /locus_tag="VLC50_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013132475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator
                     ligand-binding domain-containing protein"
                     /protein_id="HSF26913.1"
                     /translation="MGNVAKAYAAAPGDLPGAGGRVGLVYGLPLRAVLQSASLAGARV
                     LAGAAGLDRVVSRVNVMEVPDILPWVKPDELLLTTGYPLRESAHELPELVTALHERGL
                     AALAVKLGRYLQDLPAAMLERADELGLPIVRLPDDIAFDDVLNPVLTDVLNRQAAMLA
                     SSEAVHRALVQIVLAGGGLDEVCSELSRLLDAVVFVTSPDGRILAQAGAPEAIEAATS
                     APCFDASGRFRTEFERRGISTHEGLDGSHALVPVVAGSLDHGRLVAVAERMLDASDIH
                     ALEGASTVAALAITKSLAVRAVEEKYRGDFLRDVLAGRAGSPGDVVAHAAGLGWDLDR
                     PVAVLVAELDPVVGSPLSRSVDGLALRPVRERFTTAWQSVLAARDPFAAVAGFSKEVV
                     AVIGAPPGRDLERAVRELTAAVSGDGGGGRRSFSTGVSRVVSAPGTDRGALPGAYEQA
                     RTAVRVGRHLHGAGALAHFDGLGVLRLLSLIPDGHEIDAFVAETLGRLADLEDAEATD
                     LRRTLRVLLDHNLNVAEAARMLHFHYNTLRYRIGKLEGMLGPFTTDPGLRLDLMLALQ
                     VIGLREDARRS"
     gene            7119..8078
                     /locus_tag="VLC50_05285"
     CDS             7119..8078
                     /locus_tag="VLC50_05285"
                     /inference="COORDINATES: protein motif:HMM:NF022834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HSF26914.1"
                     /translation="MAVAPTAPTRAPAPGTVLGAAAHEGLRAPMHAPTRRATPLAATR
                     AAVILAVPGERPVAVVSADAVRLPGSMVLGAPSGHLGLAAFGPGTPAWIGDGRVQVGD
                     LVVRPTRWWRSRTPHRATAATLATSLAQLTKVMEPVGHEPRLTARLEAGQHELAAAVL
                     AALDPARRPSVGGSAVAAAALERTRAAAVGLLGLGPGLTPSGDDMTSGTLIAVRRLMP
                     STADEIAAIGSAIATLALSRTSPVSAALLSWAGLGEGVPELLAFVDGLGTGSDPAPAL
                     AALARVGHTSGRDLAQGAVAGARIVLELLRPSPETAELVINPA"
     gene            8161..9636
                     /locus_tag="VLC50_05290"
     CDS             8161..9636
                     /locus_tag="VLC50_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FdrA family protein"
                     /protein_id="HSF26915.1"
                     /translation="MPSVVEVRHGSYHDSVSLMQVSKAVSLVPGVRAALVAMGTELNL
                     DLLAGMGFGPAPSASPSDLLVAVEAADDESLQAALSTCDAELAALSRGSAHDGSGSLA
                     PARSLGSAARRLDATLALVSVPGPQAFVEAMDALDAGLSVMLFSDNVPVEAEIALKDT
                     AAARDLLVMGPDCGTAVVGGVGLGFANVVRPGSVGLVAASGTGAQQMLSLLDAAGVGV
                     SHCLGVGGRDLSAAVGARSTRAALAALDADPETDMLVVVSKPPAPEVAEAVRAYAATL
                     STPVVLVLIGEGQPDLTAGAEKVLAARGEAVPQWPSWQPSEPRPPRPGALRGLFSGGT
                     LCDEAMVIAAATLGTVRSNIPLRPEWALPADLRSPDHLMIDFGDDALTQGRAHPMIDP
                     TLRLERLAAEVTDPATGVVLLDVVLGHGSHPDPGRELADVVRRSEVPVVVSLCGTESD
                     PQGLSRQAEALASAGAEVYLSNAAAARRAVTLVEQAKENHQ"
     gene            9633..>10224
                     /locus_tag="VLC50_05295"
     CDS             9633..>10224
                     /locus_tag="VLC50_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020550880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HSF26916.1"
                     /translation="MSAPTTSLSGLLSTTPRVVTAGVSLLADAVSAQAVPTEPVDWRP
                     PMPGTDAALARVMADPRRRAANELAVGRLLASQSQLVDVLPASEVLGLTPGTFLHAGP
                     PITWERASGPMRGALMAAMLFEGLADTPEDAASKLERGEGITLDPCHHHATVGPMAGV
                     VSASMWMFVVEDPVHGHRAYCSLNEGLGKVLRYGAYS"
BASE COUNT         1409 a         3703 c         3733 g         1379 t
ORIGIN      
        1 cgtgcacctg cccctcgacg atcatcgggt tgatcagcgg gccgcagtcg tgcacgacgc
       61 agtagcgaag gatcttggtc tcggcggtgc ccgggtcggt ctcgacgatg accgcgtgca
      121 tgccgttggc aaacgtcgac tgggtcggcg agtagtagtc cttgccctcc agacccggct
      181 cgtcgtcgtc ggccaccggc ggcttgctga tgtcgccgac gacgaactgg gtcgccgcct
      241 tggcggcgtc gtcgaacgcg tagcgcagcg ggttggacag gaccgccacc gtcgcgagcg
      301 ggatcgagga gtcgggcgag cccttgaccc gcacgacgcc ctcgacgatc tcgaggtcgc
      361 cggggtcggc ctccagcgcc tcgccggcga tccgcagcgc cttggcgcgc acgttgcggg
      421 ccgccaacgc gatcgcgttg ccgctcatca ccgcggcccg cgaggcgaac gtgcccaccg
      481 cgtagccgaa ccggcgggtg tcgccggtga cgacctcgat gtcctcgatc ggcacgccca
      541 gctcgtcggc gacgatctgg gcgaacaccg tctggtgacc ctggccctgc gaggtcagcc
      601 cggtggcgac cttgaccttg ccgctggtct ccacctggac gtgcccgcct tcgtaagggc
      661 cgacgccggt cccctcgacg tagcaggcca ggccgatccc gacctggcgg ccctcggccc
      721 gggccgcctc acggtaggcg gtgaagtcgt cccagccgat gagcttggtg agcttttcca
      781 acgacgccgg gaagtcgccg gagtcgtaga tgagcgggcg gccgtcctgg aagatcagcc
      841 cgtggtcgta gggcatctcg tcgggctgga tgaagttgcg cgcccggacc accgtgcggt
      901 cgatgcccag gtagtcggcg atcgcgtcca tcgtgcgctc catcgcgaag acgccctggg
      961 ggcgcccggc tccgcggtag ggggtcacga tgacggtgtt ggtgtagagc gaccagacct
     1021 ggcagcggta ggcctccggc ttgtaggggc cgagcagctg ggtcgacgtg atgatcggca
     1081 cgatgatgcc gtagggcgtg taggccccgt ggtcgtgcca gaccttgacg tccagcccga
     1141 gcagtcgtcc ctcgtcgtcg aaacccacgg tgacgtgctg gagctggccc cgctcgtggg
     1201 cggccgagac gaagtgctcg cgccggtcct cggtccactt cacgccacgc ggctcgtagg
     1261 cggccagccg gcgcgccgcc caggagacga ggatctcctc gggccacggg tgcatgatct
     1321 tgacgccgaa cccgccgccg acgtcgggcg cgatgcactc gaccttggtc agcggcagct
     1381 cgagcttggc cgcgatcgcc gcgcgcaccg aggtggaggc ctgcgtcgag gagtagaccc
     1441 gcaggctgga gtcctcggcg tcccagcggg cgtagacgcc cttgccctcc atcggggtcg
     1501 acgcgctgcg cgagaagtcg aggtcgagct cgagcacgtg cggggccttg cggagcgcgg
     1561 cctcgacgtc gccgacgtcc tgcacgagat gcgcggccac gttgtcgggg atgtcctcgt
     1621 ggacggcgtg cacggccgcc cgggcggtcg cgatgccgac caccggcggc agcgggtcgt
     1681 aggtgacgac gatgcggccc acggcgtcct cggccacgta gcggtcgcgg gccaccacca
     1741 tgacgatggc ctcgccgacg tggttgacca cctcgggagc cagcgcatag gcggtgcgcg
     1801 gcgcgtgcag cgacgggtgc gggatgagca gcggcagcgg ctcggccacc cggccctcga
     1861 ggtcctcgta ggtgtagacg gcgacgacgc cgtcgacgtc gagcgccccg gtcacgtcga
     1921 tgtcgacgat gcgcgcatgg gcgtgcgggc tgcgcaggaa cgccgcctcg agcgcgccct
     1981 gccccaggtc gtcgacgtag cggcccttgc cgaccaccag ccgcaggtcc tcgcggcgct
     2041 ggacgggagc cccgaacatc ttggtggtca ccgggcaccc tcctcccgct cgcggcggat
     2101 ctgcgcggcc cgtaggaccg acgcgacgat gttctggtag ccggtgcagc ggcacagatt
     2161 gccggacacg gcctcgcgcg cggccgcctc ggtgggtgcg gggttgtcct cgaggaaggc
     2221 cgcgatggtg gtcaggaagc ctggcgtgca gaagccgcac tgcaggccgt ggcactcccg
     2281 gaaggcctgc tgcacggggt gcagcgtgcc gtcggggtcg gccagtccct cgatcgtggt
     2341 cacgtcgtgg ccgtcgacgg tcaccgcgag cagcaggcag gaccgcaccg gggtgccgtc
     2401 gagcagcacc gtgcaggccc cgcacacccc gtgctcgcac ccgacgtggg tgccggtcag
     2461 gccgagatcg tgccgcagac agtcggagag cagacgccgg accgggactg tcacctcgtg
     2521 aagcacaccg ttgacccgca gccgcaccgc catcacctgc tcggacgccg cgtcggcggc
     2581 ggtggtcact ggctggctca cagcgctccc tccgtgctca ggcccgcggc gtggcgggcg
     2641 gcctcggcca gtcctcgggc ggtgagcacc tcgacgagcc ggcttcggta gtcggcgctg
     2701 gcatggatgt cgggctcggg ttcagtgcgc gcggccgcca aggcaccggc ggcggcccag
     2761 tcaccggcgt cgtacgtggc cccggcgagc gcctcggtca ggtcgagcag agccggggtg
     2821 ggggccaccg agatgtaggc ggcccgggcg gcgcggatgc gcaggtcgtc gtcgagctcg
     2881 accaatgagg cgaccccgac cagcgcgtag tcgccgtgcc gtcgggcgct ctcgaggaag
     2941 ctcgtgccgg tcgccgccct gcgccggggg aaccggaccg cctggaccat ctcccccacc
     3001 tgcagggacg tctcgagcgg gccgacgaag aagtcggcgg cggcgatgtc gcgtcgcccg
     3061 tgcgacgaca cggcctcgac cgtgccgccg agcagggtca gcaccgcggt catctcgccc
     3121 gaggggtcgg cgtgggcgat gctgcccacc gtcgtgcctc ggttgcggat ggtggggtgg
     3181 gcgacctgac gcagggcctg gcgcagcaac ggctgcgcct cgtaggccgc ctcgtgacgc
     3241 tcgacctcgg cgtgccgggc gagggcaccg acgtggacgg catcgggggc gacccgcacg
     3301 taggcgagct cgtcgaggcg gttgatgtcg atgagccgcc caggggcggc caggcgcatc
     3361 gacagcaggg gcagaaggct ttggccacct gcgagcacct tgccgtcatg tcctgcctcg
     3421 gcgagcgcga tgaccgcgtc ctcaaggctc ctcggcgccg tgtaggtgaa cggcgagggc
     3481 ttcacagtga cgatccttcc gtggaaatgc aggggccgcg cggcccttgg tggaacaggt
     3541 gccgcgcggc cccgagcggc tcagctggtg tgcgacgcgc cgcccttgtc ctgctccgag
     3601 acctcggggt aggggtcggg cacgccgtgg gtgcccggtt cgacaagcgt cgtgccgacc
     3661 accgcgacgg cggactcggc gaggtgcgcc ggggcgagcc accgagccac gtgccatccg
     3721 agaacggtga cgatcgtgcc gagcgcgatg cccgagaggg agaagctgtc ggtgaagacc
     3781 agtgaggtgt cgccgatcgc gatgatgatg ccggcggcca ccgggaccaa gttgaccggg
     3841 ttgccgaagt cgacctggtt ctccttccag atcttggcac cgagcaggcc gatcatgccg
     3901 tagaggatca cggtgatgcc tccgagcacc ccgccgggtg tggcggagat gaccgccccg
     3961 aacttcggcg agaagccgaa gatgatcgcg acagcggctg cgacgaagta ggcggccgtg
     4021 gaatagaccc tcgttgccgc catgacgccg atgttctcgg cgtaggtcgt ggtcggcgag
     4081 ccgccgacgc tgctggcgat gatggtgccg acaccgtcgg cggcgatcgc cttgcccatg
     4141 accgggtcga ggtcggtgtt ggtcatctcg gcgacggcct tgacgtggcc ggtgttctcg
     4201 gcgatgagcg cgatgacggc cggcagcacg agcagcacga acgtccactt gatgtccggg
     4261 gtgtgccagc cgacgatcga ctccttgccg tcggcgagca tcgtcttctg cgggaagccg
     4321 aaccaggcgg cgtcggcgac gccggaccag ttgacccgga agtgggtcgt gacctcgccg
     4381 gcgccggcgt cgaacgaggt gatctgcccg aacgcccggt cgaagatcca ggagagcgcg
     4441 tagcccagga ccaggccgag gaagatcgcg atgcggccga agaacccgcg gaagccgacg
     4501 gtcatcccga tgacggccgc catgacgatg agcgcgatcc actggtcctg cggccagtag
     4561 atgttggcca cgaccggcgc caggttgaac ccgatgagca tgaccaccgc gccggtgacc
     4621 accggtggca gcaccgcgta gatgacccgc gcgcccgcga agtggatgat cacaccgacg
     4681 agcgcgagga cgacaccggc gatgaggatc gcgccggtga cgttggccga gctgccctcc
     4741 tgcgcccgga tcgcggcgac accaccgacg aacgcggcgc tggtgccgag gtagctcggg
     4801 accttgccct gcacgatcag caggaagcag atcgtcgcga tgccgctcat caagatggcg
     4861 agctgcgggt tgagccccat gatgatcggg aagacgaacg tggcaccgaa catcgccacg
     4921 acgtgctgag ctcccagccc cacggtcttg ccccaggaca gtcgctcgtc gggccggacg
     4981 acgtcgtccg gtccggcgat gctgccatcg ccgtgcagct tccaaccgat tgccatgccg
     5041 cccctcctag ggctctgacg aagcaaagtc cgcgcggtcg gtgaccatac gcggcccccg
     5101 tgaggcgacg atccggggtg gctcaccgtc gacgcgcaag cagtgatcgt cgtggtgggc
     5161 gtgacccagg tcacccaaca gggcagcgtg ccggaaagtt acggctccgg cgagcaggcg
     5221 gcaccggaaa gaactcacaa ggactggccg cgggattcgt cacttgcccc cagtggcgcg
     5281 gattgtccgc cgccaccggc gttcagctgc ggcgggcgtc ctcgcgcaac ccgatcacct
     5341 gcagggcgag catgaggtcc agccgcagcc cgggatcggt cgtgaacggg ccgagcatgc
     5401 cctcgagctt gccgatccgg tagcgcagcg tgttgtagtg gaagtgcagc atccgggcgg
     5461 cctcggcgac gttgagattg tggtcgagca gcacccgcag cgtgcgccgc agatcggtcg
     5521 cctcggcgtc ctcgaggtcg gccagccgac ccagcgtctc cgcgacgaag gcgtcgatct
     5581 cgtgcccgtc ggggatcagg ctcagcaggc gcagcacgcc gagcccgtcg aagtgcgcca
     5641 gcgcaccggc accgtgcaga tggcgcccga cccgcaccgc cgtacgcgcc tgctcgtagg
     5701 caccgggcag cgcgccccga tcggtgcccg gcgccgagac gacgcggctg acgccggtgg
     5761 agaacgatcg ccgcccgccg ccaccgtcgc cggacacggc ggcggtgagc tcgcgcacgg
     5821 cccgctccag gtcgcgcccg ggcggagccc cgatcaccgc cacgacctcc ttgctgaacc
     5881 cggccacggc cgcgaacggg tcgcgggcgg ccagcacgct ctgccacgcg gtggtgaacc
     5941 gctcccgcac cggccgcagc gccagcccat cgaccgaccg ggacaacggc gagcccacca
     6001 ccgggtcgag ctcggcgacg aggacggcca ccggccggtc caggtcccag cccagcccgg
     6061 ccgcatgcgc gaccacgtca ccggggctgc ccgcgcgccc ggccagcacg tcgcgcagga
     6121 agtcgccgcg gtacttctcc tcgaccgcgc gcaccgccag cgacttggtg atcgccagag
     6181 ccgcgacggt ggacgcgccc tcgagggcat ggatgtcgga ggcgtcgagc atccgctcgg
     6241 ccaccgccac gaggcgaccg tggtcgagcg agccggctac cacgggcacg agtgcatggc
     6301 tgccgtcgag accctcgtgg gtgctgatgc cccgtcgctc gaactcggtg cggaaccgcc
     6361 cggaggcgtc gaagcagggt gccgaggtgg ccgcctcgat cgcctcggga gcgcccgcct
     6421 gggccaggat gcgtccgtcg gggctggtga cgaagacgac cgcatcgagc aggcgggaca
     6481 gctcggagca cacctcgtcg agcccgccgc ccgccaggac gatctgcacg agggcgcggt
     6541 ggaccgcctc ggagctggcc agcatcgcgg cctgccggtt aagaacgtcg gtgagcaccg
     6601 ggttgagcac gtcgtcgaag gcgatgtcgt cgggcaggcg cacgatcggc aggccgagct
     6661 cgtcggctcg ctcgagcatg gcggccggca ggtcttgcag gtagcgaccc agcttgaccg
     6721 ccagggcggc cagcccgcgc tcgtgcagcg ccgtgaccag ctcgggcagc tcgtgagccg
     6781 actcgcgcag cgggtagccg gtcgtcagca gcagctcgtc gggcttgacc cagggcagga
     6841 tgtcgggaac ctccatgacg ttgacccggg acaccacgcg gtcgagtccg gcggcgccgg
     6901 ccagcacccg ggcaccggcc aggctggccg actgcagcac cgcgcgcagc gggagcccgt
     6961 agaccagacc gacccgcccg cccgcccctg gcagatctcc cggagcagct gcataagcct
     7021 tggcaacatt gcccatgggg atgacgctat cgcgcgccgt acggtcgaaa gaggccccgt
     7081 acggcgcggg gcctgccgag cggtccggag gtgacgtggt ggcggtcgca ccgacggccc
     7141 cgacgagggc ccccgccccg ggcaccgtgc tcggcgccgc cgcccacgag ggcctgcgtg
     7201 ccccgatgca cgcacccacc cggcgggcca ccccgctggc cgcgacccgt gctgccgtga
     7261 tcctggcggt gccgggtgag cgccccgtcg ccgtggtgtc cgccgacgcc gtccggctgc
     7321 ccggctcgat ggtgctcggc gcgccctcgg gccaccttgg cctggccgcg ttcggcccgg
     7381 gcaccccggc ctggatcggc gacggacgcg tgcaggtggg cgacctcgtc gtgcgcccga
     7441 cccgctggtg gcgctcccgc accccgcacc gggccaccgc cgccaccctg gccacctcgt
     7501 tggcccagct caccaaggtg atggagcccg taggtcacga gccgcggctc accgcccggt
     7561 tggaggccgg ccagcacgag ctcgccgcgg ccgtcctggc cgcgctcgac ccagcgcgcc
     7621 ggccttcggt gggcgggtcc gcggtggcgg cagcggccct cgaacgcacc cgggcggccg
     7681 cggtcggcct gctcgggctc ggccccgggc tgacccccag cggcgacgac atgacctccg
     7741 gcacgctcat cgccgtacga cgtctgatgc cctccaccgc cgacgagatc gccgccatcg
     7801 gctccgcgat cgccacgctc gccctcagcc gcacctcgcc ggtctcggcc gcgctgctgt
     7861 cctgggccgg cctgggcgaa ggggtgcctg agctgctcgc gttcgtcgac gggctgggca
     7921 ctggcagcga ccccgccccc gcgctggcgg ccctggctcg ggtcgggcat acgtcaggac
     7981 gtgacctggc ccagggcgcg gtcgccggcg cccggatcgt ccttgagctg ctccgtccct
     8041 cccccgagac cgcagagctg gtcatcaacc cggcctgact cccgtccccg tcacggccag
     8101 tcgcccccga cagccgtttc cccgcacccc acccgttgat cacaccagga ggaagccact
     8161 gtgccgagcg tcgtcgaggt ccgtcacggc agctatcacg actccgtgag cctcatgcag
     8221 gtcagcaagg cggtgagcct ggtccccggt gtgcgcgccg cgctggtcgc catgggcacc
     8281 gagctcaacc tcgacctcct ggcgggcatg gggttcggcc ccgcgccctc ggcgagcccc
     8341 tccgacctgc tggtcgcggt cgaggcggcc gacgacgagt cgctgcaggc cgccctgagc
     8401 acctgtgacg ccgagctcgc cgcgctcagc cggggctcgg cccatgacgg ctccggctcg
     8461 ttggcgcccg cgagaagcct cggctcggcg gcccgtcggc tcgacgcgac gctggcgctg
     8521 gtctcggtgc ccggcccgca ggccttcgtc gaggcgatgg atgcgctgga cgccgggctg
     8581 tcggtgatgc tgttcagcga caacgtgccg gtcgaggccg agatcgcgct caaggacacc
     8641 gccgcggccc gcgacctgct cgtcatgggg cccgactgcg gcaccgccgt ggtcggcggg
     8701 gtcgggctcg gcttcgccaa cgtcgtccgg cccggctcgg tgggcctggt tgccgcctcg
     8761 ggcaccgggg cccagcagat gctcagcctg ctcgacgcgg ccggggtggg tgtcagccac
     8821 tgcctcggcg tcggcgggcg cgacctgtcg gcggcggtgg gtgcccgctc cacccgggcg
     8881 gcgctggccg ccctcgacgc cgaccccgag accgacatgc tggtcgtcgt gtccaagcca
     8941 ccggcgccgg aggtggccga ggcggtccgc gcctatgccg cgaccctgtc caccccggtg
     9001 gtgctcgtgc tgatcggcga gggccagccc gacctgaccg ccggcgccga gaaggtgctc
     9061 gccgctcgcg gcgaggcggt gccgcagtgg ccctcgtggc agccgtcgga gccgcgcccg
     9121 ccccggccgg gtgcgctgcg agggctgttc agcggcggca cgctctgcga cgaggcgatg
     9181 gtcatcgcgg ccgcgacgct gggcaccgtg cgctccaaca tcccgctacg ccccgagtgg
     9241 gcactgccgg ccgacctgcg ctcccctgac cacctcatga tcgacttcgg tgacgacgcg
     9301 ctcacccagg gccgcgccca cccgatgatc gacccgacgc tgcggctcga gcggctggcc
     9361 gccgaggtca ccgaccccgc caccggcgtc gtgctgctcg acgtggtgct cgggcacggc
     9421 tcgcaccccg acccgggccg cgagctcgcc gacgtcgtac ggcgtagcga ggtcccggtc
     9481 gtcgtgtcgc tgtgcggcac ggagagcgac ccgcagggcc tgtcccgtca ggccgaggcg
     9541 ctggccagcg ccggcgccga ggtttacctg tccaacgcgg ccgccgcgcg ccgcgccgtc
     9601 acgctcgtcg agcaggcgaa ggagaaccac cagtgagtgc cccgacgacg agcctttccg
     9661 ggctgctgag caccaccccg cgggtggtca ccgccggcgt gagcctgctc gccgacgcgg
     9721 tgagcgcgca ggccgtgccg accgagccgg tcgactggcg tccgccgatg ccgggcaccg
     9781 acgccgcgct ggcccgggtg atggccgacc cgcgccggcg cgcggccaac gagctggcgg
     9841 tcggccggct gctcgcctca cagtcgcagc tggtcgacgt gctgccggcc tccgaggtcc
     9901 tcggcctgac gcccggcacg ttcctgcacg ccgggccgcc gatcacgtgg gagcgggcca
     9961 gcgggccgat gcgcggcgcc ctgatggccg cgatgctctt cgaggggctc gccgacacgc
    10021 cggaggacgc ggccagcaag ctcgagcgcg gcgagggcat caccctcgac ccgtgccacc
    10081 accacgccac ggtcggcccg atggccgggg tggtcagcgc gtcgatgtgg atgttcgtcg
    10141 tcgaggaccc ggtgcacggg caccgggcct actgctcgct caacgagggc ctgggcaagg
    10201 tgctgcgcta cggcgcctac agcc
//
LOCUS       DATIBB010000137         3545 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_1396914, whole genome shotgun sequence.
ACCESSION   DATIBB010000137 DATIBB010000000
VERSION     DATIBB010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3545
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1396914"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..720
                     /locus_tag="VLC50_05300"
     CDS             <1..720
                     /locus_tag="VLC50_05300"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015883118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HSF26917.1"
                     /translation="GGLRIDHVIGLFRLWWVPAGHDPADGTYVRADHEALVGILALEA
                     AQAGALVIGEDLGTVEPWARDYLRERGVLGTSVLWFEREADGTPRRPADWRELCLATV
                     NTHDLPPTAGYLAGEHVDLRARLGLLTRPVDQERAVDEAERRQWLDLLVTLGLCRPGA
                     STAEVVEALHRFLSWTPARLLGVALADVTGERRPINQPGTCTEYPNWRRPLGDGAGHP
                     VLLEELLTSPRLAALARAVSG"
     gene            829..1158
                     /locus_tag="VLC50_05305"
     CDS             829..1158
                     /locus_tag="VLC50_05305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF26918.1"
                     /translation="MPFLLFTASPAFAYIRDDGDDPGPGLTVLETIGIYVVAPALLVS
                     LIVALVYLPSMVRGPRYRPGLQWWASPTTFEGSPQAAVAGAEGAAEAVEAGASTSGEG
                     GGASARW"
     gene            1145..1537
                     /locus_tag="VLC50_05310"
     CDS             1145..1537
                     /locus_tag="VLC50_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020577295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5130 family protein"
                     /protein_id="HSF26919.1"
                     /translation="MPAGEPFTARQRDDIDRAVRLGEKQGQMHIKVYVGPLEGDSRQT
                     AQRLHAELGPAAAGSVLVAVDPVARAVEIVTGANLARRLPDRDCALAAMTMTSSFSLG
                     DLAGGIVDGIRTLAEHARAPRTLHLEAH"
     gene            complement(1557..>3545)
                     /gene="pepN"
                     /locus_tag="VLC50_05315"
     CDS             complement(1557..>3545)
                     /gene="pepN"
                     /locus_tag="VLC50_05315"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019630279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HSF26920.1"
                     /translation="YITALVAGPYHAVRRELTSRDGRTIPLGVFCRRSLAEHLDADAI
                     IEVTTQGFAFYEELFDYPYPFEKYDQLFVPEYNAGAMENAGAVTLLEDYVFRSRVPDA
                     MYERRAETILHELAHMWFGDLVTMRWWDDLWLNESFATYASVLCQSQATRWRHAWTTF
                     ANAEKSWAYRQDQLPSTHPISADIRDLEDVEVNFDGITYAKGASVLKQLVAWVGHDAF
                     FEGIRRYFRAFEWGNTSLADLLGKLEETSGRDLSAWSKQWLETAGVNTVRPAFETDGA
                     GRFTSFAIRQEASPARPTLRSHRLAVGLYDRVDGRLARRERVELDVADARTSVDALVG
                     QARPDLVLLNDDDLTYAKIRLDDRSLATLTSSIAAFDDSLPRTLGWAAAWDMTRDQEM
                     AARDFITLVLEGIHAETDSMVVLYLLRQLSSAVQLYTAPDYRATATARAAAGLARLMH
                     DAPAGSDHQLQFTRAFAAHAETDEQLALLTALLDGRETLPGLSIDTDLRWTLLHRLAA
                     TGRADEDAIAAELANDDTAAGRRHAASARAAMPTAEAKAAAWAAVVESDELPNATQMA
                     IITGFWVPGQERLLEAYVDPYFAAIKDVYATRTNEIAQNIVVGLYPTLLADARVVERT
                     DAWLADADIAPALRRMVVESRDGVARALRCQARDAEAS"
BASE COUNT          446 a         1273 c         1318 g          508 t
ORIGIN      
        1 ggggggctgc ggatcgacca cgtgatcggg ctgttccggc tgtggtgggt gcctgccggg
       61 cacgacccgg ccgacggcac ctacgtgcgc gccgaccacg aggcgctggt gggcatcctg
      121 gcgctggagg ccgcgcaggc gggggcgctg gtcatcgggg aggacctggg caccgtcgag
      181 ccctgggcgc gcgactacct gcgcgagcgc ggcgtcctcg gcacctccgt gctgtggttc
      241 gagcgggagg ccgacggcac gcctcgccgt cctgccgact ggcgcgagct ctgcctggcg
      301 accgtgaaca cccacgacct gccgccgacg gcgggctatc tggccggcga gcacgtcgac
      361 ctgcgggccc gcctggggct gctgacccgg ccggtcgacc aggagcgcgc cgtcgacgag
      421 gccgagcgca ggcagtggct cgacctgctc gtgacgctcg ggttgtgccg ccccggggcc
      481 tcgaccgccg aggtcgtcga ggcgctgcac cggttcctca gctggacccc ggcccgtctg
      541 ctcggggtcg cgctcgccga tgtcaccggc gaacgccgcc ccatcaacca accaggcacc
      601 tgcacggagt accccaactg gcgccgcccg ctcggcgacg gcgccgggca tccggtgctg
      661 ctcgaggagc tgctgacctc gccacggctg gccgcgctgg cccgggcggt gtctggctag
      721 cgcccggcct gcgcgccacg ggtcggcgga gcggtcccat cccgcgctgt gtacggtttg
      781 ctcgtgactc ttcgctcctg cgtccgcgcc gcggcggtgt gcgcaagcgt gccgtttctc
      841 cttttcaccg cctcgccggc cttcgcctac atccgtgacg acggcgacga cccggggccg
      901 ggcctgacgg tgctcgagac gatcggcatc tacgtcgtcg cccctgcgct gctcgtcagc
      961 ctgatcgtcg cgctggtcta cctgccgtcg atggtgcggg gcccgcgcta ccgccccggc
     1021 ctgcagtggt gggcctcccc gacgacgttc gaggggtcgc cgcaggccgc cgtggccggt
     1081 gccgagggcg ccgccgaggc cgtcgaggcg ggcgcatcga ccagcggcga gggcggtggc
     1141 gccagtgccc gctggtgagc cgttcacggc ccggcagcgg gacgacatcg accgggcggt
     1201 ccggctcggc gagaagcagg gccagatgca catcaaggtc tacgtcggcc cgctggaggg
     1261 ggactcgcgg cagaccgctc agcgcctgca cgccgagctc ggcccggccg cggccggctc
     1321 ggtgctcgtc gccgtcgacc cggtggcccg cgcggtggag atcgtgaccg gtgcgaacct
     1381 ggcccgccgg ctacccgacc gcgactgcgc gctcgcggcg atgacgatga ccagctcgtt
     1441 ctcgctcggt gacctcgccg gcgggatcgt cgacggcatc cgcacgctgg ccgagcacgc
     1501 ccgcgcgccg cgcaccctgc acctcgaagc ccactagtcc cccgcgccgg cccgggctaa
     1561 ctggcctcgg cgtcgcgggc ctggcagcgc agcgcccggg ccaccccgtc gcgtgactcc
     1621 acgaccatcc ggcgcagcgc cggggcgatg tcggcatcgg ccagccacgc gtcggtgcgc
     1681 tccaccaccc gggcgtcggc cagcagggtg gggtagaggc cgaccacgat gttctgcgcg
     1741 atctcgttgg tccgcgtggc gtagacgtcc ttgatcgcgg cgaagtaggg atcgacgtag
     1801 gcctcgagca gccgctcctg gcccggcacc cagaacccgg tgatgatcgc catctgggtg
     1861 gcgttgggca gctcgtcgct ctcgacgacc gcggcccacg cggcggcctt ggcctcggcg
     1921 gtgggcatcg cggcccgggc cgaggcggcg tgacggcgcc cggcggcggt gtcgtcattg
     1981 gccagctcgg ccgcgatcgc gtcctcgtcg gcgcgcccgg tcgcggccag ccggtgcagc
     2041 agcgtccagc gcaggtcggt gtcgatcgac agcccgggca gcgtctcgcg cccgtcgagg
     2101 agggcggtca gcagcgcgag ctgctcgtcg gtctcggcgt gcgcggcgaa ggcccgggtg
     2161 aactgcagct ggtggtcgct gccggccggt gcgtcgtgca tgagccgggc gagcccggcc
     2221 gcggcgcggg ccgtcgccgt cgcccggtag tccggggccg tgtagagctg tacggcggag
     2281 gacagctgac gcagcaggta gagcaccacc atcgagtcgg tctcggcgtg gatgccctcg
     2341 agcaccaggg tgatgaagtc gcgcgcggcc atctcctggt cgcgggtcat gtcccaggcc
     2401 gcggcccagc cgagcgtgcg cggcagcgag tcgtcaaacg ccgcgatcga ggaggtcagc
     2461 gtcgccagcg accggtcgtc gaggcggatc ttggcgtagg tgaggtcgtc gtcgttgagc
     2521 agcacgagat caggcctggc ctgaccgacc agcgcgtcga cgctggtgcg ggcgtcggcc
     2581 acgtcgagct cgacccgctc gcggcgcgcc agccgcccgt cgacccggtc gtagagcccg
     2641 accgccaacc ggtgcgagcg cagcgtcggg cgggccgggg aggcctcctg gcggatcgcg
     2701 aaggacgtga accggccggc gccgtccgtc tcgaacgccg gtcgcacggt gttgaccccg
     2761 gcggtctcca gccactgctt ggaccaggcc gacaggtcac ggccggaggt ctcctcgagc
     2821 ttgccgagca ggtcggccag gctggtgttg ccccactcga aggcgcggaa gtagcggcgg
     2881 atcccctcga agaacgcgtc gtggcccacc caggccacca gctgcttgag caccgaggcg
     2941 cccttggcgt aggtgatgcc gtcgaagttg acctcgacgt cttccaggtc gcggatgtcg
     3001 gccgagatcg ggtgggtcga cggcagctgg tcctgccggt aggcccacga cttctcggcg
     3061 ttggcgaacg tcgtccaggc atgacgccag cgggtcgcct gcgactggca gagcacgctg
     3121 gcgtaggtgg cgaaggactc gttgagccac aggtcgtccc accagcgcat ggtcaccagg
     3181 tcgccgaacc acatgtgggc cagctcgtgc aggatcgtct cggcccgccg ctcatacatc
     3241 gcgtcgggca cccggctgcg gaagacgtag tcctcgagca gcgtcaccgc gccggcgttc
     3301 tccatcgccc cggcgttgta ctccggcacg aacagctggt cgtatttctc gaacgggtag
     3361 gggtagtcga acagctcctc gtagaacgcg aacccctgcg tggtcacctc gatgatcgcg
     3421 tccgcgtcga ggtgctcggc cagcgagcgg cggcagaaca ccccgagcgg gatggtccgc
     3481 ccgtcacggc tggtcagctc acgccgtacg gcgtggtagg gcccggccac cagcgcggtg
     3541 atgta
//
LOCUS       DATIBB010000138         2973 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_1922890, whole genome shotgun sequence.
ACCESSION   DATIBB010000138 DATIBB010000000
VERSION     DATIBB010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2973)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2973)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2973
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1922890"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1581)
                     /gene="carB"
                     /locus_tag="VLC50_05320"
     CDS             complement(<1..1581)
                     /gene="carB"
                     /locus_tag="VLC50_05320"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017557795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HSF26921.1"
                     /translation="MPRRDDLRHVLIIGSGPIVIGQAAEFDYSGTQAVRVLKAEGLRV
                     SLVNSNPATIMTDPELADATYIEPITPETVAKVIARERPDALLATLGGQTALNTAMAL
                     HEMGVLEDYGVELIGANVEAIRAGEDRQRFKAIVDEIGGESARSTICHSMDECLAAVA
                     DLGYPVVVRPSFTMGGLGSGIARDEDQLRRIAGAGLAASPTTEVLLEESILGWKEYEL
                     ELMRDGADNVVVVCSIENLDPMGVHTGDSITVAPAMTLTDREYQRLRDISIDVIRAVG
                     VDTGGCNIQFAVNPVDGRVIVIEMNPRVSRSSALASKATGFPIAKIAAKLAVGYTLDE
                     IRNDITGETPASFEPALDYVVVKVPRFAFEKFPAADPTLTTTMKSVGEAMAIGRSFTE
                     ALQKALRSIDRRASVLSWAGAPADPAGLTALLERVATPYDGRLRDVHQALRAGATVEA
                     LHAATGIDPWFLDQLALIEEVATEVAAAPELTAALLRRAKRHGFSDAAIASLRGLDEA
                     VVRGIRHALGVRPVYKTVD"
     gene            complement(1581..2771)
                     /gene="carA"
                     /locus_tag="VLC50_05325"
     CDS             complement(1581..2771)
                     /gene="carA"
                     /locus_tag="VLC50_05325"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007263027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HSF26922.1"
                     /translation="MSARTPALLVLEDGRTFHGESYGAPGETYGEAVFATGMTGYQET
                     LTDPSYHRQVVVQTAPHIGNTGVNDEDVESARVWVAGYVVRDPSRVASSWRSQRSLED
                     ELASYGVVGISGVDTRALTRHLRERGAMRVGISTVEADPERLLARVLDSPAMTGADLA
                     AQVSTAEPYVVPAVGQRRFTVAALDLGVKAMSPRRLAERGIEVHVLPATATIADVLAV
                     GPDGVFLSNGPGDPATADDAVALTRGVLERAIPLLGICFGNQILGRALGFGTYKLRYG
                     HRGINQPVQELATGRVEITAHNHGFAVDAAVGQVLETPYGRAEVSHVCLNDGVVEGLR
                     CLDVPAFSVQYHPEAAAGPHDASYLFDRFAALLAAHPTRDHAVVRPAQLHDHGEVGRD
                     GGQR"
     gene            complement(2768..>2973)
                     /locus_tag="VLC50_05330"
     CDS             complement(2768..>2973)
                     /locus_tag="VLC50_05330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF26923.1"
                     /translation="WIPAETLRGWGWAHGLAGRVVPGESLLVLTWEHGGTSLDTGVFP
                     RAAADRSALAEAVGALLPAGSAS"
BASE COUNT          398 a         1082 c         1083 g          410 t
ORIGIN      
        1 gtcgaccgtc ttgtataccg gccgcacccc gagggcatgg cggatgccgc gcaccaccgc
       61 ctcgtcgagc ccacgcaggc tggcgatcgc ggcgtcggag aagccgtgcc gcttggcccg
      121 gcgcagcagc gccgcggtca gctcgggcgc cgcagccacc tcggtggcga cttcctcgat
      181 cagcgcgagc tggtcgagga accaggggtc gatgccggtg gccgcgtgca gcgcctcgac
      241 ggtggcgccg gcccgcagcg cctggtgcac gtcgcgcagc cgcccgtcgt acggcgtggc
      301 gacccgctcg agcagcgcgg tcagccccgc cgggtcggcc ggcgcgccgg cccaggacag
      361 cacgctggcg cgccgatcga tgctgcgcaa cgccttctgc agcgcctcgg tgaagctgcg
      421 cccgatcgcc atcgcctcgc cgacgctctt catcgtcgtg gtcagcgtcg ggtcggcggc
      481 cgggaacttc tcgaacgcga accgcggcac cttgaccacg acgtagtcga gggccggctc
      541 gaacgacgcc ggggtctcgc cggtgatgtc gttgcggatc tcgtcgaggg tgtagccgac
      601 ggccagcttg gcggcgatct tggcgatcgg gaacccggtg gccttgctgg ccagcgcgct
      661 cgagcgggag acccgcgggt tcatctcgat gacgatgacc cgcccgtcca ccgggttgac
      721 cgcgaactgg atgttgcagc cgccggtgtc cacgccgacc gcgcgaatca cgtcgatgga
      781 gatgtcgcgc agccgctggt actcccggtc ggtcagcgtc atcgccggcg cgacggtgat
      841 cgagtcgccg gtgtgcacgc ccatcgggtc gaggttctcg atcgagcaga cgaccacgac
      901 gttgtcggcg ccgtcgcgca tcagctcgag ctcgtactcc ttccagccga ggatcgactc
      961 ctcgagcagc acctcggtgg tcgggctggc ggccagcccg gcgccggcga tgcggcgcag
     1021 ctggtcctcg tcgcgggcga tgcccgagcc gagcccaccc atggtgaagg acgggcggac
     1081 gacgaccggg tagccgaggt cggcgaccgc ggccagacac tcgtccatcg agtggcagat
     1141 cgtgctgcgc gcgctctcgc caccgatctc gtcgacgatc gccttgaacc gctggcggtc
     1201 ctcgccggcc cggatcgcct cgacgttggc gccgatcagc tcgacgccgt agtcctcgag
     1261 cacccccatc tcgtgcaggg ccatcgcggt gttgagcgcg gtctgcccgc cgagggtggc
     1321 cagcagcgcg tccgggcgct cgcgggcgat caccttggcc acggtctccg gggtgatcgg
     1381 ctcgatgtag gtggcgtcgg ccagctccgg gtcggtcatg atcgtggccg ggttggagtt
     1441 gaccaggctg acgcgcagcc cctcggcctt gagcacccgc accgcctggg tgccggagta
     1501 gtcgaactcg gccgcctgcc cgatgacgat cgggccggac ccgatgatca gcacgtggcg
     1561 caggtcgtcg cggcgcggca tcagcgctgc cccccgtccc gacccacttc cccgtgatca
     1621 tgcagttgtg cgggacgcac aactgcatga tcacgggtgg ggtgggcggc gaggagcgcg
     1681 gcgaatcggt cgaacaggta ggacgcgtcg tgcgggcccg cggccgcctc ggggtggtac
     1741 tgcacggaga acgccggcac gtcgaggcag cgcagcccct cgacgacccc gtcgttgagg
     1801 cagacgtggg agacctcggc ccggccgtag ggcgtctcga gcacctgccc gaccgccgcg
     1861 tcgacggcga acccgtggtt gtgcgcggtg atctcgaccc tcccggtggc cagctcctgc
     1921 accggctggt tgatgccgcg gtgcccgtag cgcagcttgt aggtgccgaa cccgagcgcc
     1981 cggcccagga tctggttgcc gaagcagatg cccagcagcg ggatcgcccg ctcgagcacc
     2041 ccgcgggtca gcgcgaccgc gtcgtcggcg gtggccgggt cccccggccc gttggagagg
     2101 aaaaccccgt cggggcccac ggcgagcacg tcggcgatcg tcgcggtggc cggcagcacg
     2161 tgcacctcga tgccccgctc ggccaaccga cgcgggctca tcgccttgac gcccaggtcg
     2221 agcgcggcca cggtgaaccg gcgctgcccg accgcgggca cgacgtaggg ctcggcggtg
     2281 ctcacctggg cggccaggtc ggctccggtc atcgcgggcg agtcgagcac ccgggccagc
     2341 agccgctcag ggtcggcctc gaccgtggag atgcccaccc gcatcgcgcc gcgctcacgc
     2401 aggtggcggg tcagtgcgcg ggtgtcgacg ccagagatcc ccaccacgcc gtacgacgcc
     2461 agctcgtcct cgagcgagcg ctgggagcgc caggaggagg caacccggga cgggtcgcgc
     2521 accacgtagc cggccaccca gacccgggcc gactcgacgt cctcgtcgtt gacgccggtg
     2581 ttgccgatgt gcggggcggt ctgcacgacc acctgccggt ggtaggacgg gtcggtcagc
     2641 gtctcctggt agccggtcat cccggtggcg aacaccgcct cgccgtacgt ctcgccgggc
     2701 gcgccgtagg actcgccgtg gaaggtgcgc ccgtcctcga gcacgagcag ggccggggtt
     2761 cgagcgctca ggaggcgctc cccgcgggca gcagggcccc gaccgcctcg gccagcgccg
     2821 accggtcggc ggccgcccgg gggaacacgc cggtgtccag gctggtgccg ccgtgttccc
     2881 aggtgaggac cagcagcgac tcgccaggca cgacccgccc ggccagcccg tgcgcccagc
     2941 cccacccgcg cagcgtctcg gccgggatcc aca
//
LOCUS       DATIBB010000139         6068 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_83333, whole genome shotgun sequence.
ACCESSION   DATIBB010000139 DATIBB010000000
VERSION     DATIBB010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 6068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6068
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_83333"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(409..1266)
                     /locus_tag="VLC50_05335"
     CDS             complement(409..1266)
                     /locus_tag="VLC50_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012084617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HSF26924.1"
                     /translation="MSRTPAWPPAPEPLPVPVADNHCHLDMVADIGEAYDGVVGLSVD
                     EALARAAAVGVTRIVQIGCDLPSARWSVQTASSYPSILAGVAIHPNEAPRLHARGELD
                     AALAEIEVLSRHPRVRVVGETGLDHFRTDADGWAVQEAAFRAHIDIAKRTGRALQIHD
                     RDAHEDVLRVLESEGAPDTVIFHCFSGDADMARRAVEAGYLLSFAGTVTFKNAGGLRE
                     ALKVTPLEHLLVETDAPFLAPAPHRGQPNASYLIPRTLAVMAQVLDVDLASLCTAIDA
                     TTERVFGPF"
     gene            complement(1270..3084)
                     /gene="metG"
                     /locus_tag="VLC50_05340"
     CDS             complement(1270..3084)
                     /gene="metG"
                     /locus_tag="VLC50_05340"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019135088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HSF26925.1"
                     /translation="MPRSVLTAVAWPYANGPRHIGHVSGFGVPSDVFSRYSRMAGDDV
                     LMVSGTDEHGTPILVQAEREGVSPKELADRYNRVIVQDLHALGLSYDLFTRTTTRNHY
                     AVAQEMFATLHRNGYMVERTTMGAISPSTGRTLPDRFIEGTCPICGYDGARGDQCDNC
                     GNQLDPIDLVNPRSRINGEAPTFVETEHFFLDLPALSDALGSWLQTRTDWRPNVLKFS
                     LNLLDDIRPRAMTRDIDWGVPVPLPGWQENGAKRLYVWFDAVIGYLSASVEWARRTGD
                     GDEWRRFWCDPAAASYYFMGKDNITFHSQIWPAELLGYNGKGARGGAPGPLGELQLPT
                     EVVSSEFLTMEGRKFSSSRGVVIYVKDFLSRYDPDALRYYIAVAGPETHDTDFTWAEF
                     LRRNNDELVAGWGNLVNRTASLISKNLGSIPEAGQLLPEDRRLLAETANAFGTVGELI
                     GRHRQKHAITEAMRVVGEANKYLSDQAPWKLKNDDPDRMRTVLHVAAQAVDDAKTLLT
                     PFLPHSAQRVHETMGGTGVWSPMPQIREVDDLDGGSPYPVLMGDYDAARVAHWRSTPI
                     VPGTPVAAPTPVFRKLDPSVVDEELARLEAEAGGPAAG"
     gene            complement(3095..3969)
                     /gene="rsmI"
                     /locus_tag="VLC50_05345"
                     /pseudo
     CDS             complement(3095..3969)
                     /gene="rsmI"
                     /locus_tag="VLC50_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020579761.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
     gene            4008..5531
                     /locus_tag="VLC50_05350"
     CDS             4008..5531
                     /locus_tag="VLC50_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010469780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HSF26926.1"
                     /translation="MTSAPATVTGRRASVLRERLVARMPSDRVLGWVLPLSIALVGGI
                     LRFWHLRVPKTYVFDETYYAREAWRLLNYGVEYDVDKGAPEYVVHPPLGKWVIAVGEW
                     IFGDNSFGWRFSVAALGVVSIVMVARIARRMFRSTLLGAVAGLLLCLDSLHYVMSRTA
                     LLDSVLMFFVLAAFGALVVDRDRARARLGLTAIVAPPGRFGPGQGLRPWRLAAGLFLG
                     MACATKWSGLWFVVVFGLMTVLWDVGARRAAGVRRPFVGALLRDAAPAFAALVVLPLL
                     VYVASWTGWFLSDAEHAYFRTWAAADSGRGWPIPGGDALASLWHYHAEAYSFHVNLHD
                     NHPYKANPWSWLVLGRPISYFYEGPKLGESGCDVAQCSQAILAVGNPVLWWGAALALP
                     VVVALWLGRRDWRAGAILAGLVAGYLPWFLYQARTIYYFYAVVFVPFLVLAVTMVLGV
                     ILGGPSASPRRRTWGAAVAGAYVLLAVVTFWWLFPVLTAQVIPYSEWQLRMWLPSWV"
     gene            5786..6034
                     /locus_tag="VLC50_05355"
     CDS             5786..6034
                     /locus_tag="VLC50_05355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF26927.1"
                     /translation="MHSLFENAVVLGIGVNIGGNNANYIVAVDGITFGTATGTTVFDF
                     TDKNDCKAGGWVPNFPNTQFKNQGDCVSSLTSSARSAR"
BASE COUNT          803 a         2128 c         2221 g          916 t
ORIGIN      
        1 cggaagggta acgggcggga ggcctcgcgc gcaaaattct caagaaatcc gcgtcggtgc
       61 ctaacgcggc ctcactgccc tgacggggca cttgtgccgc tcccgtggcc cttcgactcc
      121 agatctaccc ggccgctcgg cgcccaaact gccaccgccg ctgcgcgccg cccgccgcgc
      181 gtcccgccgc cgcccggcac ccgcctggcc gccacgcccc gccgcgcggc ggtgccccgc
      241 acagcggaca caagggacgg accaacgacc gaacgcggcc tgattagcgc acagaaggcg
      301 cgtttgtccg tccttagtgt ccggccccgg cacctggcct ccgccaatcg cgcatgatcg
      361 ctgcaggtgg gggcggggtc gcgccgccgg cagcgcaggc cgccgcggct agaaggggcc
      421 gaagacccgc tcggtggtcg cgtcgatcgc ggtgcacagg ctggccaggt cgacgtcgag
      481 cacctgagcc atcaccgcca gggtgcgcgg gatgaggtag gacgcgttgg gctgcccgcg
      541 gtgcggggcc ggcgccagga acggcgcgtc ggtctcgacc agcaggtgct cgagcggggt
      601 gaccttgagc gcctcgcgca gcccaccggc gttcttgaac gtcaccgtgc cggcgaagga
      661 gagcagataa ccggcctcga cggcgcgccg agccatgtcg gcgtcgccag agaagcagtg
      721 gaagatcacc gtgtccgggg cgccctcgct ctcgagcacc cgcaacacgt cctcgtgggc
      781 gtcgcggtcg tggatctgca acgcccgccc ggtgcgcttg gcgatgtcga tgtgcgcccg
      841 aaacgccgcc tcctgcaccg cccagccgtc ggcgtcggtg cggaagtggt cgagcccggt
      901 ctccccgacg acgcgcaccc gcgggtggcg agacagcacc tcgatctcgg ccagcgcggc
      961 gtcgagctcg ccgcgcgcgt gcagccgggg cgcctcgttg gggtggatgg ccaccccagc
     1021 gaggatcgag ggataggacg aggccgtctg caccgaccag cgggccgagg gcaggtcgca
     1081 gccgatctgc acgatccgcg tcactccgac cgcggcggcc cgcgccaacg cctcgtcgac
     1141 gctgagcccg acgacaccgt cgtaggcctc accgatgtcg gcaaccatgt cgaggtggca
     1201 gtggttgtcg gccaccggca ccggcaacgg ctcgggggcc ggcggccacg cgggggtgcg
     1261 gctcatcggt cagccggccg ccggcccgcc ggcctcggcc tcgagccggg ccagctcctc
     1321 gtcgaccacc gacgggtcga gcttgcggaa caccggggtg ggggccgcga cgggggtgcc
     1381 gggcacgatc ggggtcgagc gccagtgcgc cacccgggcc gcgtcgtagt cgcccatgag
     1441 caccggatag ggcgagccgc cgtcgaggtc gtcgacctcg cgaatctgcg gcatcggcga
     1501 ccagacgccg gtgccgccca tcgtctcgtg cacccgctgc gcggaatgcg gcaggaacgg
     1561 agtgagcagc gtcttggcgt catcgaccgc ctgggccgcg acgtgcagca cggttcgcat
     1621 gcggtcgggg tcgtcgttct tgagcttcca cggcgcctgg tcggacaggt acttgttggc
     1681 ctcgccgacc acccgcatcg cctcggtgat cgcgtgcttc tgccggtgcc ggccgatcag
     1741 ctcgccgacg gtgccgaacg cgttggcggt ctcggcgagc agccgccggt cctccggcag
     1801 cagctggccg gcctcgggga tgctgccgag gttcttggag atcagcgacg cggtgcggtt
     1861 gacgaggttg ccccagccgg cgaccagctc gtcattgttg cgccggagga actccgccca
     1921 ggtgaagtcg gtgtcgtggg tctccgggcc ggcgacggcg atgtagtagc gcagggcgtc
     1981 ggggtcgtag cgggacagga agtccttgac gtagatcacc acgccgcggg aggaggagaa
     2041 cttgcggccc tccatggtga ggaactcgct ggagaccacc tcggtgggca gctgcagctc
     2101 acccagcggg ccgggtgcgc cgccccgggc gcccttgccg ttgtagccga gcagctcggc
     2161 cggccagatc tgggagtgga aggtgatgtt gtccttgccc atgaagtagt aggacgcggc
     2221 cgccgggtcg caccagaacc ggcgccactc gtcgccgtcg ccggtgcgcc gggcccactc
     2281 gaccgaggcc gacaggtagc cgatcaccgc gtcgaaccag acgtagagcc gcttggcgcc
     2341 gttctcctgc caacccggca gcggcaccgg cacgccccag tcgatgtcgc gggtcatcgc
     2401 ccgcgggcgg atgtcgtcga gcaggttgag cgagaacttc aagacattgg ggcgccagtc
     2461 ggtgcgcgtc tgcagccacg acccgagcgc gtcggacagc gccggcaggt cgaggaagaa
     2521 gtgctcggtc tcgacgaacg tcggcgcctc gccgttgatc cggctgcgcg ggttgaccag
     2581 gtcgatcggg tcgagctggt tgccgcagtt gtcgcactgg tcgccgcggg cgccgtcgta
     2641 gccgcagatc gggcaggtgc cctcgatgaa ccggtcgggc agggtgcgcc cggtcgacgg
     2701 gctgatcgcg cccatcgtgg tgcgttcgac catgtagccg ttgcggtgca gggtggcgaa
     2761 catctcctgc gcgacggcgt agtggttgcg ggtcgtcgta cgcgtgaaca ggtcgtagga
     2821 cagcccgagc gcgtgcaggt cttggacgat cacccggttg tagcggtcgg ccagctcctt
     2881 gggggagacg ccctcgcgct cggcctggac caggatcggg gtgccgtgct cgtcggtgcc
     2941 gctgaccatg agcacgtcgt cgccggccat gcgcgagtag cgggagaaca cgtcggaggg
     3001 cacgccgaaa ccggagacgt gaccgatgtg acggggcccg ttggcgtagg gccaggcgac
     3061 ggcggtgagg acggaacgcg gcatggcctc gatcctaggg gcgcgccgcg tgcccctcgc
     3121 gccccgcggt gatcgcggcg ttgtagaccc ggcggcgcgg caggccggtg gtctcggcga
     3181 ccgcggtgac ggcgtccttg cgggtcagcc cgccctcgac ctgctcggcg accatggcgg
     3241 ccaggtcggc gtcgtcgtgc tcgacgacct ggggcacggc accggcgacg accacggtga
     3301 tctccccgcg gaccccgtcg gcggcccagg ccgcgagctc ggtcatcggc ccgcgccgta
     3361 cctcctcata ggtcttggtc agctcgcggc agaccgcagc cgcccggccg gcaccgaacg
     3421 cggcgaccat gtcggccagg gttgcggcca gccggtgcgg ggcctcgaag aacaccatgg
     3481 tccgcggctc gccggccagc tcgcgcagcc gcagcgcccg cggcccgccc ttgcggggca
     3541 ggaacccctc gaagcagaac cggtcgacgg ccagcccgga gaccgcgagc gcggccagca
     3601 ccgcggacgg accggggacg acggtgaccg gcaggtcggc ggccaccgcg gcggacacca
     3661 gcacgaagcc cgggtcggac acgctgggca tgccggcgtc ggtgaccacc agcaccgtcg
     3721 cgcccccgcg catcgcctcg accagctcgc gggtgcgggc gccctcgttg tgctcgtggt
     3781 gcgagacgac ccgcccgccg accaccacgc cgagctcggc ggccagccgg cgcaagcgcc
     3841 gagtgtcctc ggcggccacc acgtcggggt gcgcaacgcc tcgaccagcc gggccgacgc
     3901 gtcaccgggc gcaccgatcg gggtcgcggc cagcaccagc cgcccggtcg gtcgcgtcgg
     3961 tgcggtcatg cgccgatcct gccagcctgc ctgcctacga tgacccggtg acctcggccc
     4021 ccgcgaccgt gaccggccga cgggcctcgg tgctgcgtga gcgcctggtc gcccgcatgc
     4081 cctccgaccg ggtgctcggc tgggtgctgc cgctgtcgat cgcgctggtc ggcggcatcc
     4141 tgcggttctg gcacctgcgg gtgcccaaga cctacgtctt cgacgagacc tactacgccc
     4201 gcgaggcctg gcggctgctc aactacggcg tcgagtacga cgtcgacaag ggcgcaccgg
     4261 agtacgtcgt acatccgccg ctgggcaagt gggtgatcgc ggtcggggag tggatcttcg
     4321 gcgacaactc gttcggctgg cggttctcgg tggccgcgct cggcgtggtc tcgatcgtca
     4381 tggtggctcg gatcgcccgg cggatgttcc gctcgacgct gctcggcgcg gtggccgggc
     4441 tgctgctgtg cctcgactcg ctgcactacg tgatgagccg tacggcgctg ctcgactcgg
     4501 tgctgatgtt cttcgtgttg gccgcgttcg gcgcgttggt cgtcgaccgc gaccgcgccc
     4561 gggcccggct gggccttacc gcgatcgtcg cgccacccgg ccggttcggc cccgggcagg
     4621 ggctgcggcc gtggcggctg gccgccggac tgttcctcgg gatggcgtgc gccaccaagt
     4681 ggagcgggct gtggttcgtc gtggtgttcg gcctgatgac ggtgctctgg gatgtcggcg
     4741 cgcgccgggc ggccggcgta cggcgaccgt ttgtcggcgc cctgctccgc gatgccgccc
     4801 cggcgttcgc cgcgctggtc gtcctgccgc tgctggtcta cgtcgcgtcg tggacgggct
     4861 ggttcctctc cgacgccgag cacgcctact tccgcacctg ggcggccgcc gactcggggc
     4921 gcggctggcc gatcccgggc ggcgacgcgc tggccagcct gtggcactac cacgccgagg
     4981 cctacagctt tcacgtcaac ctccacgaca accaccccta caaggccaac ccgtggagct
     5041 ggctggtgct gggccggccg atctcctact tctacgaggg acccaagctc ggcgagagcg
     5101 gctgcgacgt ggcccagtgc tcgcaggcga tcctggccgt cggcaacccg gtgctgtggt
     5161 ggggcgcggc gctggcgctg ccggtggtgg tcgcgctgtg gctcggccgg cgcgactggc
     5221 gggccggcgc gatcctggcc gggctggtgg ccggctacct gccgtggttc ctctaccagg
     5281 cccgcaccat ctactacttc tatgcggtcg tgttcgtgcc gttcctcgtg ctcgcggtga
     5341 ccatggtgct cggcgtgatc ctcggcgggc cgtccgcgtc gcctcggcgg cgcacctggg
     5401 gcgcggcagt ggcgggggcc tatgtgctgc tcgccgtggt gaccttctgg tggctgttcc
     5461 ccgtgctgac cgcccaggtg atcccctaca gcgagtggca gctgcgcatg tggctgccca
     5521 gctgggtctg aggcgccgac ctggcctcgg gcggcctcgg gcgaatgacc acgccaagta
     5581 ggggttgctg cactaggcaa ggcgtgcacg ccttgcctag tgacaggctg cctacttcac
     5641 gtggtcattt caccgaggcg aactgtggca ttcgggtgat acgcctggtg cgtgacgaat
     5701 tggggcgtaa catcactcgc ggcgtccggg caggacccgg cgttggtgag gcaatggagg
     5761 cgcccccttc tacaccctcg acgacgtgca ctcccttttc gagaatgcgg tagtactagg
     5821 catcggcgtg aacatcggcg gcaacaacgc caactacatc gtcgccgtcg acgggatcac
     5881 gtttggcacc gccactggca ccaccgtgtt cgacttcacc gacaagaacg actgcaaggc
     5941 gggcggctgg gtgccgaact tcccgaacac acagttcaag aaccaaggag actgcgtgtc
     6001 ctccttgacc tccagcgctc gctccgctcg ctgacgcgag cagcggtcgc atgcggcgtc
     6061 ggcgggcg
//
LOCUS       DATIBB010000140         2382 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_1135023, whole genome shotgun sequence.
ACCESSION   DATIBB010000140 DATIBB010000000
VERSION     DATIBB010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2382)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2382)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2382
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1135023"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1665)
                     /gene="menD"
                     /locus_tag="VLC50_05360"
     CDS             complement(<1..1665)
                     /gene="menD"
                     /locus_tag="VLC50_05360"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013770093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HSF26928.1"
                     /translation="MNPSTAMAQVIMSQLFNGGVREVVLSPGSRSAPLAVALHEADVA
                     GWMRLHVRIDERSAGFLALGLAKASGQPVPVVCTSGTAAANLHPAVLEAHHAGVPLVV
                     LTADRPPELRGVGANQASELQPTVYGGAVRLAVELALASGRVGEVAYWRATLARVLAA
                     ATGRRSSDPGPVHVDVPFREPLLPDGDTTWVEPIVGPPHSVVMSAERPSGEGRVRLGQ
                     GPRTVVVAGDGAGWQARWLAEAAGWPLLAEPSSGARSGPNALACYRLLLERLGGDIER
                     VVQFGHPTLSRPISRLLARDDVELVVVSSTPRWSDAGHRAALVAPSIGLDWVAAGAGR
                     AEPDAWLRRWLDADAAACQAVRCVLDDSPLSGPRVAAELAAALPPQALLMVGSSNPVR
                     DLDLADPWDDPLPLDDAPPARRVLANRGLAGIDGTVSTAIGAALAVQRERPGSSYALL
                     GDLTFLHDANGLVLGPDEPRPDLTLVVVNDDGGGIFTTLEQGGPEHAGSFERVFGTPH
                     HVDLAALCAATGTAHERLQTVEGLRKALVPRPGLRVVEVPIARTDRR"
     gene            complement(1662..>2382)
                     /locus_tag="VLC50_05365"
     CDS             complement(1662..>2382)
                     /locus_tag="VLC50_05365"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018656247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HSF26929.1"
                     /translation="TVLARFPGCTTVKVKVADGGRTDPATVAADLARLEAVRAALGPA
                     GLVRIDLNGAWSLDDAVRLLPIYAKAAGGLEYAEQPCATVEDLAALRRRVDVPIAADE
                     SIRRAEDPLRVARLGAADVAVLKVQPLGGVRACLRIAEQIRLPVVVSSALETSIGIAA
                     GVALAAALPELPHACGLATVGLLERDVVADPLVAVQGMIAVRRPAPDPDALAATRADA
                     ATEARWRERMAAVEAAGVDAR"
BASE COUNT          326 a          929 c          871 g          256 t
ORIGIN      
        1 ccgccgatcg gtgcgggcga tgggcacctc gaccacgcgc aatcccggcc gcggcaccaa
       61 cgccttgcgc aacccttcga cggtctggag ccgctcgtgc gccgtacccg tcgccgcgca
      121 caacgccgcg aggtcgacat ggtgcggcgt gccgaagacc cgctcgaagc tcccggcgtg
      181 ctccgggccg ccctgctcga gtgtggtgaa gatgccgccg ccgtcgtcgt tgaccacaac
      241 cagcgtgagg tccgggcgcg gctcgtcggg gccgagcacc agcccgttgg cgtcgtgcag
      301 gaacgtcaga tcacccagca gcgcgtacga cgacccgggc cgctcccgtt gcacggccag
      361 cgcagccccg atcgccgtcg acaccgtgcc gtcgatgccg gccagcccgc ggttggccag
      421 cacccgccgc gccggtgggg cgtcgtctaa gggcagcggg tcgtcccagg ggtcggccag
      481 gtcgaggtcg cggaccgggt tggacgagcc gaccatcagc aacgcctgcg gcgggagggc
      541 ggcggcgagc tcggcggcca cccgcgggcc ggacagcggc gagtcgtcga gcacgcaccg
      601 caccgcctgg catgcagccg cgtcggcgtc gagccagcgg cgcagccagg cgtccggctc
      661 ggcccggccg gcccccgcgg cgacccagtc cagcccgatc gagggcgcga caagggccgc
      721 ccggtgcccg gcgtccgacc atcgcggcgt cgacgagacc accaccagct cgacgtcgtc
      781 gcgggccagc agccggctga tcgggcgcga cagcgtggga tgcccgaact ggaccacccg
      841 ctcgatgtca ccgcccaggc gctcgagcag aagccgatag caggcaaggg cattggggcc
      901 cgagcgggca ccggaggacg gctcggccag cagcggccag ccggccgcct cggcgagcca
      961 gcgagcctgc cagccggcgc cgtcgccggc cacgacgacg gtgcgcggac cctggccgag
     1021 cctgaccctg ccctcccccg acgggcgttc cgccgacatg accacgctgt gcggaggtcc
     1081 gacgatcggc tccacccagg tcgtgtcgcc gtcgggcagc agcggctcgc ggaacggcac
     1141 gtcgacgtgc accggcccag ggtcactcga gcgccgaccg gtcgccgcgg ccaggacgcg
     1201 cgccagcgtg gcgcgccagt aggccacctc tccaacccgg cccgaggcca gcgcgagctc
     1261 gacggctagc cgcaccgcgc cgccgtacac cgtgggttgc aattcgctgg cctggttggc
     1321 cccgaccccg cgcagctccg gcgggcgatc ggcggtcagc acgaccaggg gtacgccggc
     1381 gtggtgggcc tccaggaccg ccgggtgcag gttggccgcc gcggtgcccg aggtgcacac
     1441 caccggcacc ggctgcccgg aggccttggc cagccccagc gcgagaaacc cagcagagcg
     1501 ctcgtcgatg cgcacgtgca gccgcatcca tccggccaca tcggcctcgt gcaaggccac
     1561 cgccagcggc gcgctgcgcg agcccggcga gagcaccacc tcgcgcaccc cgccgttgaa
     1621 cagctggctc atgatcacct gggccatcgc cgtcgatggg ttcatcgcgc gtccacccct
     1681 gcggcctcca cggcggccat cctctcccgc cagcgcgcct cggtggcggc atcggcccgg
     1741 gtggccgcga gcgcgtcggg atcgggcgca ggacgtcgta cggcgatcat gccctgcacc
     1801 gcgacgagcg ggtcggcgac gacgtcgcgc tccagcaggc cgaccgtcgc cagcccgcag
     1861 gcgtgcggca gctcgggcag cgcggcggcc agcgcgaccc cggcggcgat gccgatgctg
     1921 gtctccagcg cgctcgagac caccaccggc agccggatct gctcggcgat ccgcaggcac
     1981 gcgcgcaccc cgccgagcgg ctgcaccttg agcacggcca cgtcggcggc gccgagccgg
     2041 gccacccgca gcgggtcctc ggcgcggcgg atcgactcgt cggcggcgat gggcacgtcg
     2101 acccggcggc gcagcgcggc caggtcctcg accgtggcgc agggctgctc ggcgtactcc
     2161 agaccgccgg ccgccttggc gtagatgggc agcaggcgca cggcgtcgtc gagcgaccat
     2221 gcgccgttga ggtcgatgcg caccaggccg gccggaccga gcgcggcgcg caccgcctcg
     2281 agccgggcca ggtctgcggc gacggtggcc ggatcggtgc gcccgccgtc ggcgaccttg
     2341 accttgaccg tcgtacatcc ggggaaccgc gcgaggacag tc
//
LOCUS       DATIBB010000141        26131 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_1529785, whole genome shotgun sequence.
ACCESSION   DATIBB010000141 DATIBB010000000
VERSION     DATIBB010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 26131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26131
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1529785"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1011)
                     /locus_tag="VLC50_05370"
     CDS             complement(<1..1011)
                     /locus_tag="VLC50_05370"
                     /inference="COORDINATES: protein motif:HMM:NF014647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family protein"
                     /protein_id="HSF26930.1"
                     /translation="MRKNRLVAAVAGIPLALGLVACGGDGATGSPDATGDTPFRVAAL
                     LTGTENDGAISQAFVEGMKKAKDEHGIEIRMVGPVQSPDETMQQGAAFARDGYDLVFV
                     VHSGLAESIPKLATEFPDTQFCAFFDEDPDKLKTQPTNVCYFDPEHQYGSFMGGVAAG
                     LSTKTNHVGAVVGMDIPLATRQAQGFHLGAKCVNPNVRYTEAFTGDFNDSAKAKTAAQ
                     SLYGAGADILIAAVDSAVQGLFTVAESMQDRYVVPQYFDNHDAAPDVVLTSVLFGLDE
                     IAQEIIEDAMAGSVERQYRFTDKFSLADFREHADVIGEDNIAKLEEIRAKVLAGEIKI
                     PDE"
     gene            complement(1050..2042)
                     /locus_tag="VLC50_05375"
     CDS             complement(1050..2042)
                     /locus_tag="VLC50_05375"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSF26931.1"
                     /translation="MSDQHQLVTLGVVAIAVAAPVLWAALGELVAEKGGIINPGIEGV
                     MLVAALAATTAYRSSASMLLAVLAALAAGLVAGLLFAYLFVSRGVNQIITGILFNLFA
                     LGITTTIFIAQSDLARARIKVTPPIAVPLLEDLPIVGPVLFRQNVFVYGSVLAVLAIY
                     ALMRWTWLGLAIRAAGEHPRAVEAAGLNVWRVRYVATVIGAVLPAMGGAVLVLGIVGG
                     FNPGMSSGQGFIALGIVVLARWNALAVVAGSLLFGLAQALQFQAQGMALLRGVPIEIW
                     LAVPYLVTIAAVMLTRSSEYPRAAATPYVPPARGLRLRWSRPSVLTRSPGTTAG"
     gene            complement(2052..3038)
                     /locus_tag="VLC50_05380"
     CDS             complement(2052..3038)
                     /locus_tag="VLC50_05380"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSF26932.1"
                     /translation="MAILASLAIMAITLLVLGADPVAATGSMLEGALGSSFGIGETLT
                     ITSILVLTGLAAAVPFAAGMWNIGGEGQMYAGAVAATAVAFTAEGLPAWAHVSLAVLA
                     AVAGGALWGLIPGVLKTRLGANEVIVTLMLVYVAMYGVEFTINHLWPSRAQTTPPIPS
                     SAALPTLVPGAHVSAGVLLALAAVVGAAILLRRTHLGFAIRATGDNVRAARLSAIRPD
                     RVRVLAFVIGGGFAGAAGGIAVLGVSRALVSGFSDNFGFIGIAVALMARLSPVWIVGS
                     ALLFSVLRVGANQMQVSTGLDPSTGDVLVTAFVLTLMAFGVIRVKQLGSIRE"
     gene            complement(3077..4609)
                     /locus_tag="VLC50_05385"
     CDS             complement(3077..4609)
                     /locus_tag="VLC50_05385"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSF26933.1"
                     /translation="MSSAPTISMRGITKRFAGATANADIDLDLSPGEVHALLGENGAG
                     KTTLMSILFGLLTPDAGEISVDGRAVRMRSPHQALEMGIGMVHQHFMLVPTFTVTENI
                     VLGAHFGRGPVLRAAAAERRVRETAERFDIAVDPRVLVGDLPVETKQRVEILKLLHRG
                     ASTLILDEPTSVLGPAEREALFATLSQLRERGASIVIVTHKLAEVMSIADRVTILRAG
                     RKVLAAARGDYDARTLAHAMVPQEVGALPARATTQTSDRPARLAVDALTVLTDGTRSL
                     DGVTLQVLPGEVLGVAGVAGNGQQELMDALAGLRPVDHGRIRIDGADLTAGDTRTRRQ
                     AGLGVIPADRQGWGIAASMSVAENLTMTRVGAGETGQRRGFTLRTRRAQVDELIVSYE
                     IRPTDPTLPVGALSGGNQQKVVVARELERRPRVLLVANPTQGLDIGAAALVHRKLIEA
                     RNAGCAILLVSNDLDELRELSDRLVVLFGGRIVLEGTSADLPTPTLASAMAGALEPDP
                     VP"
     gene            4879..5808
                     /locus_tag="VLC50_05390"
     CDS             4879..5808
                     /locus_tag="VLC50_05390"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013651792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HSF26934.1"
                     /translation="MTAQSAAATVGWIGAGRMGYSMAARLLDAGHPVAIWNRTRAKAE
                     PLTEQGAVVVDRPVELAGHAVVFTMVSTGADLEHVLFGDGGLLTGEARPEIVVDSSTV
                     DVEEAAALRQRAGELGVTLLAAPVSGNGKVVDAGKLTIAVSGPQDAFERVRGYLEAIG
                     RSVTYVGEDELARLVKICHNLFLGVVTQSLAEITVLAERGGVSRHDFLAFLNDSVMGS
                     VFTRYKTPAFVNLDLKPTFTPVLLRKDFDLGLAAARAMEVPIPIAAHVHQLVSAAVSR
                     GHREQDFAVLLQLQAEAAGMALRSEERDVGTGL"
     gene            5904..7541
                     /locus_tag="VLC50_05395"
     CDS             5904..7541
                     /locus_tag="VLC50_05395"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HSF26935.1"
                     /translation="MSDLTAELVVRGGRIWTGVADTPPVTALAVRDGRIAAIGTDDEL
                     APLTAGAAQVIDLQGKVLLPGLQDSHIHAVRAGLTWDRELHWEDVRSLPEALETVRAR
                     AAETAPGTWIIVIGGWHERQLAERRMPTRAELDAAAPGSPVFVQMLYDVGMLSSAALK
                     ACGWDAASADPPGGVLERDSVSGELTGRVTGMGAFGHVLSQLPEPTYDEKRGGTVRML
                     AEMASHGITAVIDGGGFRMPPESYHPLLDLWRTKSLPVRMRLFMSAATRGAEVAELAQ
                     WIRHVAPGFGDDVVRISGLGEITHYGCHDMEGLDPYEMSDEAWHELVEISRAAAARGW
                     TMSMHAVLDHTLGRVLDAWELVARDHDLRPLRFSIVHADEASLANIDRIAKLGVGVLV
                     QDRLVLKASDYVDSWGADATARTPPLGDLVGQGVPIAAGTDADRANWWHPWASIWWLV
                     TGESVDGAEPRAPRHRLSIEDALRAYSHGGAWFTFEEASRGLLVPGHAADFFVPTADP
                     FAVEPEALPGIRSELTVMGGQVTHSAGALTDAGPARA"
     gene            7671..8105
                     /locus_tag="VLC50_05400"
     CDS             7671..8105
                     /locus_tag="VLC50_05400"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HSF26936.1"
                     /translation="MGSHIEVIHHEGFDPEINSPFVPAIRVASGRLVFISGVTGAPVY
                     HDHPHVPEVFDAVPADIENQVRIAFEHLDLALAAAGCSRSDVVSMTRFFTNVRDDQDA
                     VNKHQKEWFGGHIPTTTSVEVVALATDPRLRLEIQTVAVAPV"
     gene            complement(8175..8576)
                     /locus_tag="VLC50_05405"
     CDS             complement(8175..8576)
                     /locus_tag="VLC50_05405"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HSF26937.1"
                     /translation="MEISHINPDGIYKHPAFTSIVTVSDPQKLHFFAGRTPADESYRC
                     VAPGDMLAQYRQVMNILTRELEEVGATWSDVVYRRIFTTDVDACLAAAASPEIASFFD
                     PDRMPPSTLVGVTRLSDPEFMVEIDLLAVTE"
     gene            complement(8634..10088)
                     /locus_tag="VLC50_05410"
     CDS             complement(8634..10088)
                     /locus_tag="VLC50_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HSF26938.1"
                     /translation="MVGRSATAAADELCARSATELASAIRRSEVSPVEVVDAVLARID
                     KVNPRLNAFCTVTAEQARRRAQLAADEVARGAALGPLHGVPYSLKDLTDTAGVRTTLG
                     SALFRDRVPQADAVLAERMARAGGILVGKTNTPDHGCKGVTDNPVFGQTKNPWDLTRT
                     PGGSSGGASAAVAAGMAPLAEGSDFAGSIRIPASFCGLVGYKPSVGRVPIYPNDFLWH
                     PVSFISGPITRTVADAALMLQVIAGADERDPVSLPDPTDDYLDATREEATPRSAKIAW
                     SPDLGFAMVDPEVVEVCHAAVRSAAEAIGASVTEYDLGLPDSTDAYQLLNATLRAGLM
                     EDYFPGRRDEMDPLMAWRTEHARERRASHVAVAERTQSIVYERLRTMFQTYDYLVLPT
                     TPTTAFDIELTYQREIGGSPVDSPFQTLPLTFVFNLSGHPAVSIPAGWSADGMPVGLQ
                     IVGRWRDDASVLALAAAFERVLPWRHRWPDLAST"
     gene            complement(10180..11400)
                     /locus_tag="VLC50_05415"
     CDS             complement(10180..11400)
                     /locus_tag="VLC50_05415"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HSF26939.1"
                     /translation="MDQRGSPEHRFGGGAFGRLPIGELQTSRRAFLRNVALVGLGATG
                     VAGLVSACGGDEAGGQGTAAPSGASDGGIGSLIGAAPDGTDLVKPGPFDHASETLSMD
                     DVMGFQVPKAKRRYKMAVMPQSLAGEYFIATVYAAKKAADEAGVDLTVITGEGFASPD
                     VQLSQLSDLLQKNIDAVMLMPADVNGSVALVELAHSNDIVMTVNGTLLNSTKVAQAVQ
                     SDYAAGQQCADILAETLGGSGGKGVVIAGPKQAAWASLRVAGFEARLKEKYPDITIAA
                     IANQNFVDPAEGLNTFLDAVQRVPDIDWIYAVDYNILEAGSIPDRYRGKIRYVAMGLY
                     GSSREALKAGTVNAIVGLLPGASGMLGLSRAVQQLNGEDVPAITIYPSPVYTADNIDD
                     PASVLETYPAGYKP"
     gene            complement(11461..12966)
                     /locus_tag="VLC50_05420"
     CDS             complement(11461..12966)
                     /locus_tag="VLC50_05420"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HSF26940.1"
                     /translation="MTSAAADLEVRSLTKKYAGIAAVQNVNALLPAGQVHALVGANGA
                     GKSTLIKMLSGVTRPTSGQILLGGQELVPRGPLDALKAGIATIQQDVAVAPDLTVAEN
                     ILLGREPSPGLPGFVSRRRASAVAEAILGRLGVDLAPDARVETLSHAQRGLVSIARAL
                     AFDCQVLILDEPTASLSHEAAEHLHAVIRDLRDQGKTIIYVSHRLDDVLALAQTVTVL
                     RDGRQVALLPRAEVTLDRVIRLMTGGVDLAARASGHEPIESDGGPALVARSIAAAPHF
                     TDVSLAVRAGEVLGIAGFIGSGREEVVAALAGALRLTGGAIEVSGQAVRLGSPERALR
                     HGIQLLPGDRAGGGLGLGQSIRSNVMLPPSGEVARWGIRRLRRERMRCAELLQQVGCV
                     PADPERPAGHLSGGNQQRVLMARALHLKRQILLVENPTQGVDIKGKSEIHQLILDLAA
                     TGMAVVVSSAEIDELESLCHRILVMRGGAVVGDLPVGEFDSHRLLQLASSA"
     gene            complement(12963..14024)
                     /locus_tag="VLC50_05425"
     CDS             complement(12963..14024)
                     /locus_tag="VLC50_05425"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSF26941.1"
                     /translation="MDTGTGTAMDRSGSGEASIQQRQPDRPPATVARRFARASRVLNR
                     GELSLVGFTVVVCAVLAIWQPSSFATMSNVQNMAAQGAVLGVVAIGQMFPLLVGGFDI
                     SVGAMMGVSSVVGVYLMINHGVAVGLAGGILATALLGLLNGVLIARFALSAFIVTLGM
                     LSLARGFSLTMTGGTPITNPPEAFRWFGSRDLGGIPTTVLIALVVVLVAGVILALTRM
                     GLYLYSIGSNERAARLAGVAVTRYKILSYVIASTCAGIAGLMLSSRVASGQPSLGDGY
                     DLRSIAVAVIGGVAIGGGKGRVQGVLLGLVLITVIGSGLNIADVSPFAQQMVIGLLII
                     LAMLWDRFQTRSQRGEASA"
     gene            complement(14202..15275)
                     /locus_tag="VLC50_05430"
     CDS             complement(14202..15275)
                     /locus_tag="VLC50_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020511554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HSF26942.1"
                     /translation="MTSAVTLKDVARLAGVHAGTASKALNPLTRKGVSSRTVQAVLEA
                     ASQLGYRPDPIARSLRTNRSNVVGIILPDLTNPMFPPAVRGIEDALREAGYTALVANT
                     DQDLVREEEIYRAMRGRRVDGFIIATARREHPLLREAADEGVAIVQLNRVTDDGRIPG
                     VLVDDTAGVSRAVDHLVSLGHRHLAHLAGPADLSTAESRMQAFLGACHRHGKAVARPV
                     IDNCESYSVDAGAAATEALLAKHPECTAIVAGNDLIALGSYRALAKAGLSIPGDISVV
                     GFNDMPFVDMLQPPMTTVHVPHYHLGVEAARLLLERLRDERTPAKRLLLPSDLVVRGS
                     TGAPPQERAPRRTPRRRAASQAS"
     gene            complement(15362..16555)
                     /locus_tag="VLC50_05435"
     CDS             complement(15362..16555)
                     /locus_tag="VLC50_05435"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HSF26943.1"
                     /translation="MTEPRVDPIPPAFTDAELQARRVALRAVMAARGIDVLVGYGAHR
                     AGPVVPWLTCWPVTREAVVLLGQDDPVELLVGFYNHVPEARRLARGPVTVDWCGEDTA
                     TTLAQRLTRRGRGLSVGTVGAIPARVAAALGSCASTVVPLDADHTRLRQTKSPEELTW
                     LRHAAALTDDSARALIQAATPGRSELELVAAVEAAYAGTGGANHIHYLASTSMAAPDR
                     CAPGQWPGRRVLASGDVVVFELSTTWGPDYPGQVLRTVTVTAEPTPLYCDLHAVAEAV
                     LAEIVPMLRPGVAPTQLLAAAKLVEAAGFVTVDDIVHGLGGGYLPPVLSSRSQVPHGL
                     DAEPLRAGMTLVVQPNVCTPDLQAGVQTGELFLVTDGEPVSLHRLPTGMLPGGQAPAG
                     GQAGS"
     gene            complement(16568..17386)
                     /locus_tag="VLC50_05440"
     CDS             complement(16568..17386)
                     /locus_tag="VLC50_05440"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSF26944.1"
                     /translation="MTLARSRVGKDAPTHPGQGMSEAGLRRLLWLTGLLLIPLGWQLF
                     AVIEESPLIVGPIEVAQATVSMTLDGQLPGAALSSGLVFVTGLALGTFAGLVIGILIG
                     RFRTVEVILDPYVSALFATPLVAVIPILIVVLGFGFQAKVVIVAMFAFFPVTINTAAG
                     VRAVPADFSELAQTFCSSELRTWREILVPGALPYIVTGVRLAVGRSLIAVVVAEFSTA
                     VTGLGFLILRHSRRFNMAESLVPVIVLMCAGFALYSWLKHIENRLAPWVLRPTE"
     gene            complement(17383..18150)
                     /locus_tag="VLC50_05445"
     CDS             complement(17383..18150)
                     /locus_tag="VLC50_05445"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HSF26945.1"
                     /translation="MTDQRLSPRRVLALRLGFAAALLAAWYLATMMTGSLFLAAPHEI
                     IARLAESIASGEFATYLGPTAYILVVGAGLGVGLGVPLGLLIGRVRWLYWMTEAPLNI
                     FYTTPLVALIPFILVVVGFNYTAKILIVFLFTVLTVIINTAAGVRTVDPDLLELSQAF
                     CGKGWALWRDILVPGALPAIVSGLRIGLIHALVGAVLADFYAGASGFGYLIILYSNRF
                     DIAGALGPVLVLALAGSLVAAGLKMLQRRLSPWEGGR"
     gene            complement(18143..18928)
                     /locus_tag="VLC50_05450"
     CDS             complement(18143..18928)
                     /locus_tag="VLC50_05450"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011695415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSF26946.1"
                     /translation="MTALDVDAITKDFGDLHVLDPIDLHVDDNEFVSILGPSGCGKTT
                     LLRILAGIEQASSGSVMVGGTPSTRPRLDTAMVFQNFRLLPWRTVEDNVAYGLRLRGQ
                     SKTAARAQALPFLKTVGLEGNEKKYPYQLSGGMQQRVGLARALAIGPATLLMDEPFGA
                     LDAQTRELMQDELMRIWSTDRKTVVFVTHSIDEAIVLSDRVIVMQSRPGRIVEDIPIP
                     FGRPRDPASMRISPRFAELRAQMWSLLRQKTLAPDAIGGVTGD"
     gene            complement(18995..20035)
                     /locus_tag="VLC50_05455"
     CDS             complement(18995..20035)
                     /locus_tag="VLC50_05455"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HSF26947.1"
                     /translation="MRQSLGSKHLLIGLTVAALVLMTGCSGDDSGTASEGELKSLSLS
                     MPVIPPNFVHIMPWVAQDQGFYDDFGLSVKIVSLDSGVTALRGAEAGSADIAAVPTPT
                     LINAVAQGSHVKGFYTYSPKLDVQMVVTKDISSCADLKGRVLAVDEVGGFAEVLTQQF
                     YSSCGLTKNDVKYGNFPGAEGQAMAQGQSVSGVLHIDEAAGVMEQFPNAGLKVLANLW
                     EVVPQWHYAGFAAPQEILDKKRDEIIAFTAANIKAKEFMSDRSNKDAVLDTAEEVTGL
                     SRDILSDTYDTFLKDGLWPEANGYPQDMVAYTADQQVKLGNIQESQKPAYDDVVDTSI
                     YEAALALIDEQS"
     gene            complement(20227..22521)
                     /locus_tag="VLC50_05460"
     CDS             complement(20227..22521)
                     /locus_tag="VLC50_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014174171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HSF26948.1"
                     /translation="MTVLERPARRTWRWVGQSLPRPEDHDLVRGAAPFVADIAAPEAL
                     DAVFVRSQTAHGHLVAVRTEQAARVAGVAAVWSAAELPDLPGVPPAARSQQPAAMRRP
                     ALVRDRVRFVGEPVAVVVAAGRYAAEDAAELVEVDIDPLPAVVDARAAAEADAPQLFD
                     GVSNVSSTRELGAPCTPAVEASPVVVETWVTNQRVSPMSLEARAILVIPEPNGGLTVW
                     CSHQAPHRLRAALARSFGLDPATVRVAVPSVGGAFGAKSQVYAEYLVVAAAAMRLGRP
                     VRWIEDRYEAFVAATHGRGQTTHLRLGLDRDGRFQALEARIWADVGAYPDTGDFIPQL
                     SAWVMSGPYRIPQIHVLAHSVVTTTTPTASYRGAGRPEAAFALERLVDLTADRLGLDP
                     ARLRETNLLTADELPYRSPTGAVYDSARHHDALHAAMDALDYAGWRAEQQRRRTSGER
                     RQVGIGLASWVERSGGLGGSTEYARVEVGADGTVTARIGTASQGQGHLAPFAQVVADG
                     LGVSPDAIRVRLGDTAEVAEGTGAFASRSMQVGGNAAYLAAVALRERLREAAAQRLDA
                     AVADVADADGVFTTTDGRSTSLAELAAAVPDLAEEQVFGPPQAFPFGAYAAVVEVDTR
                     TGEVAVLRLVAVDDVGVVVNPMVVSGQTVGSIAQGLGQALYEGIVYDESGQPLTTTLL
                     DYTVPTAAEMPDLRLVEMVTPNPNVPLGTKGAGESGCIGTPPALVNAVCDALRDYDTT
                     GLTMPLTARKVWECLQRPTVGPTR"
     gene            22594..23478
                     /locus_tag="VLC50_05465"
     CDS             22594..23478
                     /locus_tag="VLC50_05465"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HSF26949.1"
                     /translation="MKPQSFEYAAPATLAEACDVLASRAEEAKVLAGGQSLVPMMALR
                     LARPELVVDLNGVAGLDELSVGEDGLRLGALVRHRRLERGPVDGPLTSLLARAARHIG
                     HPPIRARGTVCGSLAHADPAAEWCLVGVATDAQVGVVSAGGRRELTVDALLDGPFSTT
                     LEPDEVIADVTFPAWRPGRHGAGLAERSRTAGGFAELAACAVLEVADATVAAAAVAVV
                     GAAGRPVRLPGTEAALVGSPAGPGGSWAGTVARAGALAVTEVPDRGDGGYAAAVLGAL
                     VEEALTQAQEEVRRWTSD"
     gene            23463..23960
                     /locus_tag="VLC50_05470"
     CDS             23463..23960
                     /locus_tag="VLC50_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HSF26950.1"
                     /translation="MDVRLTVDGRDHLVQVEARTTLADALREECGVTAVHLGCEQGSC
                     GACTVVLDGDAVRSCLVFAVQADGKRVGTAAGLADGEHAHPVQQALSEHHGLQCGFCT
                     PGFAVLLAHALDERPDLDDDELVELVSSNLCRCTGYRGILAAARSVRDQRAGSGLGAA
                     GPTGG"
     gene            complement(23925..24983)
                     /locus_tag="VLC50_05475"
     CDS             complement(23925..24983)
                     /locus_tag="VLC50_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020511554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HSF26951.1"
                     /translation="MRAPTLRDVAREAGVHPATVSRALNPSTRKLVNDATAERVARVA
                     AALGYRPNPIARSLKTARSTSIGMIIPDLTNPLFPPIVRGIEDVLAPAGYNAWLANTD
                     NDPDRAAAHVESMRSRHVEGLIIATALLEDPLLDKLAAEDLPMVLINRRLARTDVPTV
                     TSDDATGVALAVSHLAELGHRRIVHLAGPQTVSTGVTRLRSFRQAVHEHGLDDDPRLI
                     VRCSAFSVAEGARAMREILDEGVEFTAVFAGNDMQAVGVYDVLQERGLRCPEDISVVG
                     FNDTPFFDKLRPPLTTIHIPHYDIGAEAARMLLEELRDPTRSARSVLLPLTLVRREST
                     APPRRKASAARRTRRAKA"
     gene            complement(25063..>26131)
                     /locus_tag="VLC50_05480"
     CDS             complement(25063..>26131)
                     /locus_tag="VLC50_05480"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSF26952.1"
                     /translation="PLTVIVAAASAYFLAYGVTQPLWGIASDRIGLVRTMRITLLAAG
                     VFSLASAASPGVSWLLVSRTLAGGCFSAAFPAALVYVGDTVPGVRRQHEVTGLLSGSA
                     LGTAVATAGAGLLAATIGWRWMFVATGLLGLGLAFALRALPRPPRTRMGRPILAPLRD
                     VLRHRASVLVIGLAFAEGAIIIGVITYLPAAVEDGGVSPAVAGAVVGIYGLAVLVAAT
                     AVGRLSARMPPARLIVVGALATAAACALAALSTRPGPVLAVCVLLGIGWAFMHSTLQT
                     WATMVLPSARATVISLFAGALFAGSAVSSALGGSSADQGRWALIFLVALAATVPLGLV
                     AAISRHRWSPVPAEQLRAGQS"
BASE COUNT         3941 a         8963 c         9467 g         3760 t
ORIGIN      
        1 ctcgtccgga atcttgatct cgccggcgag gaccttggcc cggatctcct cgagcttggc
       61 gatgttgtcc tcaccgatca cgtcggcgtg ctcgcggaag tcggccagcg agaacttgtc
      121 ggtgaaccgg tactgccgct cgaccgaccc ggccatggcg tcctcgatga tctcctgggc
      181 gatctcgtcc agcccgaaga gcacgctggt gagcacgacg tccggtgccg cgtcgtggtt
      241 gtcgaagtac tgcggcacca catagcggtc ctgcatcgac tcggcgacgg tgaacaggcc
      301 ctggaccgcc gagtccaccg cagcgatgag gatgtccgcg cccgcgccgt acaggctctg
      361 ggctgctgtc ttggccttgg cggagtcgtt gaagtcgccg gtgaacgcct cggtgtagcg
      421 cacattcggg ttgacgcact tggcgcccag gtgaaagccc tgggcctggc gggtggccag
      481 agggatgtcc atgcccacga ccgcgccgac gtggttggtc ttggtcgaga gcccggccgc
      541 gaccccgccc atgaagctgc cgtactggtg ctcggggtcg aagtagcaga cgttggtcgg
      601 ctgcgtcttg agcttgtcgg ggtcctcgtc aaagaacgcg cagaactgcg tgtcggggaa
      661 ctccgtggcc agcttgggga tcgactcggc cagcccgctg tgtacgacga acacgaggtc
      721 atagccgtcg cgggcgaagg cggcaccctg ctgcatggtc tcgtcgggac tctggaccgg
      781 gccgaccatc cggatctcga tgccgtgctc gtccttggcc ttcttcatgc cctcgacgaa
      841 ggcctgcgag atggcgccgt cgttctcggt gccggtgagc agggcagcga cgcggaacgg
      901 cgtgtcaccc gtggcgtccg gcgagccggt ggctccgtcg ccgccgcagg cgacgaggcc
      961 gagagccagg gggatgccgg ccacggcggc cacaagcctg ttcttgcgca tggtttctcc
     1021 ttgagtgggt gggggagcga gtgctcggtc tagccggcgg tggtgccggg tgaccgggtc
     1081 aggacggatg gacgtgacca gcgcaggcgc aggccacgag ccgggggcac gtagggggtg
     1141 gcggccgcgc gcgggtactc cgaggagcgc gtcagcatga ccgccgcgat cgtcaccagg
     1201 tagggcacgg ccagccagat ctcgatgggc accccgcgca gcagcgccat cccctgggcc
     1261 tggaactgca gcgcctgagc aagcccgaac agcagtgacc cggcgaccac ggccagcgcg
     1321 ttccaccggg cgagcaccac gatgccgagc gcgatgaacc cctggccgga ggacatcccg
     1381 gggttgaagc caccgacgat gccgagcacg agcaccgcgc cgcccatggc gggcagcacc
     1441 gcgccgatca ccgtcgcgac atagcgcacc cgccagacgt tgagcccggc ggcctccaca
     1501 gctcgtgggt gctctccggc agcccggatc gccagcccga gccaggtcca tcgcatcagg
     1561 gcgtagatgg ccaggacggc gagcaccgag ccgtagacga agacgttctg ccggaagagc
     1621 accggcccca cgatcggcag gtcctcgagc aaggggacgg cgatcggcgg ggtgaccttg
     1681 atgcgggccc gagccaggtc gctctgcgcg atgaagatcg tcgtggtgat cccgagcgcg
     1741 aaaaggttga agagaatgcc ggtgatgatc tggttgaccc cgcggctgac gaacaggtag
     1801 gcgaacagca gtccggcgac cagcccggcg gcgagcgcgg cgaggacggc gagcagcatg
     1861 ctggcgctgg agcgataggc cgtcgtggcg gccagcgccg cgaccagcat gaccccctcg
     1921 atgccggggt tgatgatgcc gcccttctcg gcgaccagct cgccgagggc agcccacagc
     1981 accggagcgg cgaccgcgat cgcgacgacg cccagcgtga cgagctggtg ctggtcagac
     2041 atcgtggccc ctcattcccg gatcgagccg agctgcttga cccggatgac cccgaacgcc
     2101 atcagcgtga gcacgaacgc ggtgaccagc acgtcgccgg tggaggggtc gagcccggtg
     2161 ctcacctgca tctggttggc acccacgcgc agcacgctga acagcagggc ggaaccgacg
     2221 atccagaccg gggacagccg agccatgagc gcgacggcga taccgatgaa gccgaagttg
     2281 tccgagaacc ccgacacgag agcccggctc acacccagca ccgcgatgcc gccggcggcc
     2341 ccggcgaagc cgccgccgat gacgaacgcg aggacccgca cccggtcggg gcggatcgcg
     2401 ctcaaccggg cggcgcgcac gttgtcgccg gtggcccgga tggcaaaacc cagatgcgta
     2461 cggcgcagca ggatggccgc gccgaccacc gcggccaacg ccagcaggac acctgcgctg
     2521 acgtgggccc ccggcacgag cgtgggcagc gcggcggagg acgggatcgg cggcgtggtc
     2581 tgcgctcggc tgggccacag gtggttgatg gtgaactcga cgccatacat cgcgacgtag
     2641 accagcatca gcgtgacgat gacctcgttg gcgcccagcc gggtcttgag gacgcccggg
     2701 atgagtcccc agagcgcgcc gcccgcgacg gccgccagca cggccagcga gacgtgcgcc
     2761 caggccggca gcccttcggc ggtgaacgcg acggcggtgg cggcgaccgc gccggcgtac
     2821 atctggccct cgccgccgat gttccacatg cccgcggcga acgggacggc cgcggcgagt
     2881 ccggtcagca ccaggatgct ggtgatggtc agtgtctcgc cgatgccaaa gctgctgccc
     2941 agcgcgccct cgagcatcga gcccgtggcg gccaccgggt cggcgcccag caccagcagc
     3001 gtgatcgcca tgatggccag cgacgcgagg atcgccacgg ccgcgcgccc gtgtgggcgc
     3061 aggcgagtcc acccggtcac ggcacgggat ccggctcgag ggcgccggcc atcgccgagg
     3121 ccagcgtcgg ggtcggcagg tcggcgctcg tgccctcgag cacgattcgg ccgccgaaca
     3181 gcacgacgag ccggtcggag agctcgcgca gctcgtcgag atcgttggag accaacagga
     3241 tcgcgcagcc ggcgttgcgg gcctcgatga gcttgcggtg gaccagggcc gcggcgccga
     3301 tgtccagacc ctgggtcggg ttggccacca gcaggacccg gggtcgccgc tcgagctcgc
     3361 gggccaccac gaccttctgc tggttgccgc ccgagagcgc tccgaccggg agggtgggat
     3421 cggtcgggcg gatctcatag ctgacgatca gctcgtcgac ctgggcccgc cgcgtgcgca
     3481 gggtgaagcc acgccgctgt ccggtctcgc cggccccgac ccgggtcatg gtcaggttct
     3541 ccgcgacgct catcgacgcc gcgatgcccc agccctgacg gtcggcgggg atcacgccga
     3601 gcccggcctg ccggcgtgtc cgggtgtcgc cggcggtcag gtcggcgccg tcgatgcgga
     3661 tgcgcccgtg gtcgaccggc cgcagcccgg ccagcgcgtc catgagctcc tgctggccgt
     3721 tgcccgccac accggcgacg ccgagcacct caccggggag cacctgcagc gtcaccccgt
     3781 ccagcgatcg ggtgccgtcg gtgagcacgg tgagcgcgtc gacggccaac cgggctggcc
     3841 gatcgctcgt ctgggtggtc gcccgggcag gcaaggcccc gacctcctga ggcaccatcg
     3901 cgtgcgcgag cgtgcgggcg tcatagtcac cgcgggccgc ggcgagcacc ttgcgcccgg
     3961 ctcgcaggat cgtcacccgg tcggcgatcg acatgacctc ggcgagcttg tgggtcacga
     4021 tcacgatcga ggcgccgcgc tcgcgcagct ggctcagggt cgcaaacagt gcttcccgct
     4081 cggccggacc gagcaccgag gtgggctcgt cgaggatcag cgtcgatgca ccgcgatgga
     4141 gcagcttgag gatctcgaca cgttgcttgg tctcgaccgg gaggtcaccc acgaggaccc
     4201 gcgggtccac cgcgatgtcg aagcgttctg cggtctctcg cactcgccgt tcggccgccg
     4261 ccgcccgcag caccgggccg cggccgaaat gggcgccaag cacgatgttc tcggtcaccg
     4321 tgaacgtggg cacgagcatg aagtgctgat gcaccatgcc gatgcccatc tcgagggctt
     4381 ggtgcggcga gcgcatccgc accgcgcggc cgtccaccga gatctcgccg gcgtcggggg
     4441 tgagcaggcc gaacaggatg ctcatgagcg tcgtcttacc ggcgccgttc tctccgagca
     4501 gggcgtgcac ctcgccgggg gacaggtcga ggtcgatgtc cgcgttggcc gtcgcgcccg
     4561 caaaccgctt ggtgatgccg cgcatgctga tcgtgggggc actcgacacc aggttcgagc
     4621 ggtgagggga cactgcggct ccagtgcgct ccgccgtcgt gcgccggctg ggggacgtgc
     4681 tgatgtcctc gatgttggtc gcaatcgatt acgtgacgcg aaacctccca cgaggcgaga
     4741 gacctgtcaa gggtgaaggt atcgaggcta ttcctcatgg ttgacccctt gcacggtctg
     4801 ccttactctc acaatcgttt gcgaaaccga gtcttccctg ccgcctcggc ggggctgggt
     4861 gagaggaacc cgaggaccat gactgcacag agtgcggccg cgaccgtcgg ctggatcggc
     4921 gccggtcgga tgggctactc gatggccgcc cgactgctcg acgccggcca tccggtggcg
     4981 atctggaacc gcactcgggc caaggccgag ccgctcaccg agcagggcgc cgtggtggtc
     5041 gaccggcccg tcgagctggc cgggcacgcc gtggtgttca ccatggtctc cacgggtgcc
     5101 gacctggagc acgtgctgtt tggtgacggt ggcctgctca ccggcgaggc ccgccccgag
     5161 atcgtggtcg actcgtccac cgtcgacgtc gaggaggcgg ccgcgctgcg ccagcgggct
     5221 ggtgagctcg gcgtgaccct gttggctgcg ccggtctcgg gcaacggcaa ggtcgtcgac
     5281 gccgggaagc tgaccatcgc cgtctccggt ccgcaggacg ccttcgagcg ggtccgcggc
     5341 tacctcgagg cgatcgggcg cagcgtcacc tatgtcggtg aggacgagct ggcccggctg
     5401 gtcaagatct gtcacaacct cttcctcggc gtggtcaccc agagcctcgc cgagatcacc
     5461 gtgctggccg agcggggtgg ggtcagccgc cacgacttcc tggccttcct caacgacagc
     5521 gtgatgggtt cggtgttcac ccgctacaag accccggcgt tcgtcaacct cgacctcaaa
     5581 ccgacgttca ctcccgtctt gctgcgcaag gacttcgacc tcggtctggc ggccgcgcgg
     5641 gcgatggagg tgccgatccc gatcgccgcc cacgtgcacc agctggtctc ggccgcggtc
     5701 agccgggggc accgcgagca ggacttcgcc gtcttgctgc agctgcaggc agaggcggcc
     5761 gggatggcgc tgcgctcgga ggagcgcgac gtcggcaccg gcctctagga ggcgcccggg
     5821 ggcctcggaa cacccgtttg gtgagccttg atcggcgggt cgccctgata gcgtccggtc
     5881 accaacgaga ttccggagca gccgtgagtg acctaaccgc tgagctggtc gtgcggggcg
     5941 gacggatctg gacgggcgtg gccgacacgc ctcccgtcac cgccctggct gtgcgtgacg
     6001 gccgcattgc cgccatcggg accgacgacg agctcgcccc gctcaccgcc ggtgctgccc
     6061 aggtcatcga cctgcagggc aaggtcctgc tgcccggcct gcaggactcc cacatccatg
     6121 ccgtgcgagc cgggctgacc tgggaccgcg agctgcactg ggaagacgtg cgctcgctgc
     6181 ccgaggcgct cgagacggtg cgggcacgtg ctgccgagac cgcgcccggc acctggatca
     6241 tcgtgatcgg cggctggcac gaacgccagc tcgccgagcg gaggatgccg acccgggccg
     6301 agctcgacgc cgcggcgccc ggctcaccgg tgttcgtcca gatgctctac gacgtcggca
     6361 tgctcagctc ggcggccttg aaggcgtgcg gctgggacgc ggcctcggcc gacccgcccg
     6421 gcggagtgct cgagcgtgac tcggtcagcg gcgagctcac cggccgggtg accggcatgg
     6481 gcgcgttcgg ccacgtcctc tcgcagctgc cggagccgac gtacgacgag aagcgaggcg
     6541 gcacggtccg gatgctggcc gagatggcca gtcacggcat caccgcggtc atcgatggtg
     6601 gcgggttccg gatgccgccg gagagctacc acccgctgct ggacctgtgg cgcaccaaga
     6661 gcctgccggt gcggatgcgg ctgttcatgt cggcagccac gcgcggggcc gaggtggccg
     6721 agctggcgca gtggatccgt catgtcgcgc cgggcttcgg cgacgacgtg gtgcgcatca
     6781 gcggcctggg cgagatcacc cactacggct gccacgacat ggaggggctc gacccctatg
     6841 agatgtccga cgaggcgtgg cacgagctgg tcgagatcag ccgggccgcg gcggcccgtg
     6901 gctggacgat gagcatgcat gccgtgctcg accacacgct cggtcgcgtg ctcgacgcct
     6961 gggagctggt cgcccgagac cacgacctgc gcccactgcg gttctcgatc gtgcacgccg
     7021 acgaagccag cctcgccaat atcgaccgga tcgccaagct cggtgtcggt gtgctggtgc
     7081 aggaccgctt ggtgctcaag gccagcgact acgtcgactc gtggggagcg gatgcgacgg
     7141 cgcggacgcc tccgcttggc gacctcgtcg ggcagggcgt gccgatcgcc gcgggcaccg
     7201 acgccgaccg cgccaactgg tggcacccgt gggcctcgat ctggtggctg gtgaccggtg
     7261 aatcggtcga cggggccgag cctcgggcgc cccggcaccg gttgagcatc gaggacgcgc
     7321 tgcgggccta cagccacggc ggtgcctggt tcacgttcga ggaggcgagc cgcgggctgc
     7381 tggtgcccgg gcacgccgct gacttcttcg tgccgaccgc cgacccattc gcggtcgagc
     7441 cggaggccct gcccggcatt cgcagcgagc tgaccgtcat gggcgggcag gtcacccaca
     7501 gtgccggtgc cctgaccgat gccgggccag cgcgggccta gctcgcgcgt caatcggccc
     7561 ggcgccgggc attgtgggcc gggccgctgc tggccttgac tcctgctgcc ggccgaccta
     7621 aggtccccga aagtcatcga gacaatcgat tgtcattgaa gggaacagcc atgggctcgc
     7681 acatcgaggt catccaccac gagggcttcg accccgagat caacagtcct tttgtcccgg
     7741 cgatccgggt ggcgtcgggc cggctcgtgt tcatctccgg agtgacgggc gccccggtct
     7801 accacgacca cccgcacgtg ccggaggtct tcgacgcggt cccggccgac atcgagaacc
     7861 aggtgcggat cgcgttcgag cacctcgacc tggccctggc tgcggccggc tgctcccgct
     7921 cggacgtcgt gtccatgacc cgcttcttca ccaacgtgcg tgacgaccag gacgccgtga
     7981 acaagcatca gaaggaatgg ttcggcggtc acatcccgac gacgacttcc gtcgaggtgg
     8041 tcgccctggc gaccgacccc cgcctgcgtc tggagatcca gacggtggcc gtcgcaccgg
     8101 tgtgatcctg ccccgacgag ctcgggacga accgccgtgg ccctcgtcgc accccgcgac
     8161 gagggccacc gcgctcactc cgtgacggcc agcaggtcga tctccaccat gaactccggg
     8221 tcagacaacc gggtcacgcc gacgagcgtg ctcggcggca tccggtcggg atcgaagaag
     8281 gacgcaatct cggggctggc ggcggccgcc agacatgcgt cgacgtcggt cgtgaagatg
     8341 cgtcggtaga cgacgtcgct ccacgtggcg cccacttcct ccagctctcg agtcaggatg
     8401 ttcatcacct ggcggtactg ggcgagcatg tcgccgggcg ccacacacct atagctctcg
     8461 tccgccgggg tccggccggc gaagaagtgc agcttctgcg ggtcagacac cgtgacgatc
     8521 gaggtgaatg ccgggtgctt gtagatgccg tcggggttga tgtgtgagat ctccatggtt
     8581 tcccttcctc actccaaagg acggtccctg ggtgtggggt gtctcggccg ggtctaggtc
     8641 gaggcgagat ccggccagcg gtggcgccag ggcaggacgc gctcgaatgc ggcggcgagt
     8701 gctagcaccg aggcgtcgtc acgccaccgg ccgacgatct gcagaccgac cggcatcccg
     8761 tcggccgacc atccggccgg gatggacacg gcggggtgcc cggacaggtt gaagacaaat
     8821 gtcagcggca gggtctggaa cggcgagtcg accggggagc cgccgatctc gcgctggtag
     8881 gtcagctcga tgtcgaaggc cgtggtcggg gtcgtgggga gcaccaggta gtcgtaggtc
     8941 tggaacatgg tccgcagccg ctcgtagacg atcgactggg tccgctcggc cacggcgacg
     9001 tgactggctc gtcgttctcg ggcgtgctcc gtccgccatg ccatgagggg gtccatctcg
     9061 tcgcgccggc ccgggaagta gtcctccatc agccctgccc ggagcgtggc gttgagcagc
     9121 tggtaggcgt cggtcgagtc cggcagcccc aggtcgtact cggtcacgct ggcgccgatc
     9181 gcctctgcgg ccgagcgcac cgccgcgtgg cagacctcga ccacctcggg gtcgaccatg
     9241 gcgaacccga gatctggcga ccaggcgatc ttcgccgacc tgggcgtcgc ctcttcgcgt
     9301 gtggcgtcga ggtagtcgtc agtggggtct ggcaacgaga cgggatcgcg ctcgtccgcg
     9361 ccggcgatga cctgaagcat caacgcggcg tcggcgaccg tccgggtgat cgggccgctg
     9421 atgaatgaca cggggtgcca caggaagtcg ttcgggtaga tcggcacccg cccgacggag
     9481 ggcttgtatc cgacgagccc gcagaacgag gccgggatcc ggatcgaccc ggcgaagtcg
     9541 ctgccctcag ccagtggcgc catgccggcg gccaccgcgg ccgaggcccc gccgctggag
     9601 cctccggggg tgcgggtcag gtcccacggg ttcttggtct gaccaaagac cgggttgtcg
     9661 gtgactccct tgcacccgtg gtcaggggtg ttggtcttgc ccaccaggat gcccccggcc
     9721 cgggccatcc gctcggccag caccgcgtcg gcctggggga cccggtcgcg gaacaatgcc
     9781 gaccccaggg tggtccggac accggcggtg tcggtgaggt ccttgagcga gtagggcacc
     9841 ccgtgcaggg ggccgagcgc ggcccctcgg gccacctcgt cggcggccag ctgagcccgg
     9901 cgcctcgcct gctccgcggt caccgtgcag aaggcgttga gccgaggatt caccttgtcg
     9961 atccgggcca gcaccgcatc gaccacctcg accggcgaga cctcggacct gcggatcgcg
    10021 gacgccagct cggttgccga gcgcgcacac agctcgtctg ccgcggccgt agcgctacgt
    10081 ccgaccatga ccatcactcc ttacgacgac cagatggttg cccgctgctc ggacccgccg
    10141 gccggggcgt tgccccgacc ggcgggtccg tgcggacggt cagggcttgt accccgccgg
    10201 gtaggtctcc agcacactgg ccgggtcgtc gatgttgtcg gcggtgtaga ccggcgacgg
    10261 gtagatcgtg atcgcgggca catcctcgcc attgagctgc tgcaccgcgc gtgagaggcc
    10321 gagcatgccg ctggccccgg gcagcaggcc gacgatcgca ttgaccgttc cggccttcag
    10381 cgcctcgcgc gacgagccgt agaggcccat cgccacgtag cggatcttcc cgcggtaacg
    10441 gtccgggatc gagcccgcct cgaggatgtt gtagtcgacc gcgtagatcc agtcgatgtc
    10501 cggcacgcgc tggaccgcgt ccaggaaggt gttgagcccc tcggccgggt cgacgaagtt
    10561 ctggttggcg atcgccgcga tggtgatgtc cgggtacttc tccttgagcc gcgcctcgaa
    10621 gccggcgacc cgcagcgacg cccaggccgc ctgcttcggg ccggcgatca ccacgccctt
    10681 cccgcccgat ccgcccagcg tctcggccag gatgtctgcg cactgctggc cggccgcgta
    10741 gtcgctctgc accgcctgcg ccaccttggt gctgttgagg agcgtgccgt tcacggtcat
    10801 cacgatgtcg ttggagtgcg cgagctcgac cagggcgacc gagccgttca catcggccgg
    10861 catcagcatg acggcgtcga tgttcttctg cagcaggtcg ctcagctggc tcaactggac
    10921 gtcgggcgag gcgaaaccct caccagtgat caccgtcagg tcgacgccgg cctcgtcagc
    10981 ggccttcttg gcggcgtaga cggtcgcgat gaagtattca cccgccagcg actgcggcat
    11041 gacggccatc ttgtagcggc gcttggcctt gggcacctga aagcccatca cgtcgtccat
    11101 gctgagcgtc tcgctcgcgt ggtcgaacgg accgggtttg acgaggtcgg tgccgtccgg
    11161 cgccgccccg atcagcgagc cgatgccacc gtctgaggcc ccgctcgggg cggccgtgcc
    11221 ctgtccgcca gcctcgtcac cgccgcaggc ggagacgagg ccggcgaccc cggtggcgcc
    11281 aagcccgacc agggcgacgt tgcgcaagaa tgctcgcctc gaggtttgca gctcgccgat
    11341 cgggagccgg ccgaaggcgc ctccgccaaa cctgtgctca gggctaccac gctgatccac
    11401 gatgtgctcc ctcttctcgc ccacgcgaga tccggacatg tgttggttgg cctggcaccc
    11461 tcacgccgag gaggccagct gcagaagcct gtgactgtcg aactcgccca ccgggaggtc
    11521 gccgaccacg gcgccacctc gcatgacgag gatccggtgg cacagggact cgagctcgtc
    11581 gatctccgcc gaggagacga cgacggccat tccagtggcc gccaagtcga ggatgagctg
    11641 gtggatctcc gacttcccct tgatgtcgac cccttgggtc gggttctcca ccagcaggat
    11701 ctggcgcttc aggtggagcg cccgggccat cagcacgcgc tgctggttcc caccgctgag
    11761 atggcccgct ggccgctctg ggtcggccgg gacgcacccc acttgctgca gcagctcggc
    11821 gcaccgcatc cgttctcgcc gcagcctgcg gatgccccac cgggccacct cgcccgaggg
    11881 gggcagcatg acgttgctgc ggatcgactg acccagaccc aggccgccgc cggcccgatc
    11941 accaggcagc agctggatcc cgtgtcgcag agcgcgctcc ggcgacccga gccgcacggc
    12001 ctggccgctc acctcgatgg cgccaccggt cagccggagg gcgccggcca gcgcggcgac
    12061 gacctcctca cgcccggagc cgatgaagcc ggcgatcccc agcacctccc cggcgcgcac
    12121 cgccaggctc acgtcggtga agtgcggcgc tgccgcgatc gaccgggcga ccagggctgg
    12181 gccgccgtcg ctctcgatcg gttcgtgccc ggatgctcgg gcggcgagat cgactcctcc
    12241 ggtcatcagc ctgatgaccc ggtcgagcgt cacctcagcc cggggcagga gggccacctg
    12301 ccgaccgtcg cgcagcacgg tcaccgtctg cgccagcgcc aggacgtcgt cgagccggtg
    12361 ggagacgtag atgatggtct tgccctggtc gcgcaggtcg cggatcaccg cgtgcaggtg
    12421 ctcggcggct tcgtgcgata gcgacgccgt gggctcgtcc aggatcagca cctggcagtc
    12481 gaaggcgagc gcccgcgcga tcgagaccag accgcgctgc gcgtggctga gggtttcgac
    12541 tcgcgcgtcg ggggcaaggt cgaccccgag tcggcccaag atcgcctcgg cgacagcgga
    12601 tgctcgccgc cgtgagacga agccgggcag gccggggctc ggctcgcgcc ccagcaggat
    12661 gttctcggcg acggtgaggt cgggcgcgac ggcgacatcc tgttggatcg tggcgatgcc
    12721 ggccttcagc gcgtccagcg ggccccgcgg caccagctcc tggccaccga ggaggatctg
    12781 accggaggtg ggccgggtca cgccggagag catcttgatc agggtggact tgcccgcacc
    12841 gttcgcaccg accagagcgt gcacctgacc agcgggaagc agcgcgttca cgttctggac
    12901 ggccgcgatg ccggcgtatt tcttggtgag gctgcggacc tcgaggtcag ccgcggccga
    12961 ggtcacgcgc tcgcctcccc gcgctgcgac cgcgtctgga accggtccca gagcatggcc
    13021 aggatgatca acagaccaat caccatctgc tgggcgaacg gcgagacgtc ggcgatgttg
    13081 aggccgctgc cgatgacggt gatgagcacc aggccgagga ggactccctg cacgcggccc
    13141 tttcctccgc cgatggcaac cccgccgatc accgccaccg cgatcgaccg caagtcgtag
    13201 ccatcgccaa gactcggctg ccccgaggcc acccgcgagg agagcatgag gcccgcgatg
    13261 ccggcgcacg tgctggcgat gacgtaagag aggatcttgt agcgggtcac tgccaccccg
    13321 gcgaggcggg cggcgcgctc attgctgccg atcgagtaga ggtagagccc catccgggtg
    13381 agcgcgagga tcacccccgc gacgagtacg acgaccagcg cgatgagcac cgtcgtcggg
    13441 atgccaccca ggtcgcgtga gccaaaccag cggaaggcct ccgggggatt ggtgatcggg
    13501 gtgccacccg tcatggtgag cgagaaacca cgggccagcg acagcatccc cagggtgacg
    13561 atgaaggcgc tcagcgcgaa ccgagcgatg agcaccccgt tgagcagtcc gagcagcgcg
    13621 gtcgcaagga tgcctccggc caacccgacg gccacgccgt ggttgatcat caggtagaca
    13681 cccacgacgc tggagacgcc catcattgcg ccgaccgaga tgtcgaaacc accgaccagc
    13741 aacgggaaca tctggccgat ggcgaccacc ccgaggacgg cgccttgcgc cgccatgttc
    13801 tggacgttgg acatggtcgc gaaggacgac ggctgccaga tcgccaggac ggcacagacc
    13861 acgacggtga aaccgacgag tgacagctcg ccgcggttga ggacacggga ggcccgcgca
    13921 aatcggcggg cgactgtggc cggcggcctg tcgggctgac gctgctggat cgaagcctca
    13981 ccactgcccg aacgatccat ggccgtaccg gtcccagtgt ccatggggct gcactcctgc
    14041 ctcgtggtcc ggcacaccgt aatgtaaggg tgataggact gcaagcgatt gcgatccaag
    14101 ctcgccttgt gtggcctcga ctcagcgtca agttgcagcg atcgaaacgc aagcgattgc
    14161 gaaactggcc cttgtgcagc ggctccagtc ccggatggga gctagcttgc ctgcgatgca
    14221 gctcggcgcc gaggcgtgcg cctgggtgcg cgctcctgcg ggggtgcgcc ggtagagccg
    14281 cgaacgacaa ggtcggaggg cagcagcagt cgctttgctg gcgtgcgctc gtcgcgcagt
    14341 cgctcgagca gcagcctggc cgcctccaca ccaaggtggt agtgcggcac gtggacggtc
    14401 gtcatcgggg gctgcagcat gtcgacgaac ggcatgtcgt tgaagccgac gactgagatg
    14461 tcacccggga tcgagagccc ggccttggcc agcgcgcgat agctgccaag cgcgatgagg
    14521 tcattgccgg ccacgattgc ggtgcactcc gggtgcttgg ccagcagcgc ctcggtggcc
    14581 gccgcccccg cgtcgacgct atagctctcg cagttgtcga tgaccggccg ggcgaccgcc
    14641 ttgccatggc gatgacaggc gccgaggaat gcctgcatgc ggctctcggc cgtcgacagg
    14701 tcggccgggc cggccaggtg agcgaggtgc cggtgcccga gggagacgag atggtcgacc
    14761 gcgcgcgaca ccccggctgt gtcgtcgacg aggacgccgg gaatcctccc gtcgtcggtc
    14821 acgcggttga gctgcacgat ggcgacgccc tcgtcggcgg cctcgcgcag cagtgggtgc
    14881 tcgcgccggg cggtcgcgat gatgaagccg tcgacgcgcc gcccgcgcat cgcccggtag
    14941 atctcctctt ccctgaccag gtcctggtcg gtgttggcga ccagtgcggt gtagccggcc
    15001 tcgcgcaggg cgtcctcgat gccccggact gccggcggga acatcgggtt ggtcaggtcg
    15061 gggaggatga tgcccacgac attgctgcgg ttggtgcgca ggctgcgcgc gatcggatca
    15121 ggccggtagc ccagctggct cgcggcctcg agcacggcct gcacggtgcg actgctgaca
    15181 cctttgcggg tcaaggggtt gagagccttg gaggcagtgc cggcgtggac gccggccagg
    15241 cgggccacgt ccttgagcgt cactgccgag gtcacggcag ggacgatacc cgaccagggc
    15301 cagcaatcga atgcctcaga tggtgcggtt atggctgagc gctcggcggc gtcccggtgc
    15361 ttcagctgcc cgcttgaccg ccggccggcg cctgcccgcc gggcagcatc cctgtgggca
    15421 gccggtgcag cgacacgggc tcaccgtcgg tcacgaggaa cagctccccg gtctgcaccc
    15481 cggcctgcag gtcgggcgtg cacacgttgg gctgcaccac cagggtcatg ccggcacgca
    15541 gcggctcggc atccaggcca tggggcactt ggctgcgtga gctgagcacg ggcgggagat
    15601 agccaccgcc gaggccgtgc acgatgtcgt ccacggtcac gaaccctgcg gcctccacca
    15661 gcttggccgc ggccagcagc tgggtggggg cgacacccgg acgcagcatc ggcacgatct
    15721 cggccagcac cgcctcggcg accgcgtgca ggtcgcagta gagcggcgtc ggctcggcgg
    15781 tgaccgtgac cgtgcgcagc acctggccgg ggtagtcggg cccccaggtc gtgctcagct
    15841 cgaagacgac gacgtcgccg ctggcgagca cgcgacggcc gggccactga ccgggggcgc
    15901 aacggtcggg ggccgccatg ctggtcgagg cgaggtagtg gatgtggttg gcacccccgg
    15961 tgccggcata ggcggcctcg acggcggcga ccagctcgag ctcgctacgg ccgggtgtcg
    16021 ccgcctggat gagcgcgcgg gccgagtcgt cggtcagagc cgcggcgtgg cgcagccagg
    16081 tcagctcctc ggggctcttg gtctgccgca gccgggtgtg gtcggcatcg agggggacca
    16141 cggtcgaggc gcacgacccg agcgcggcgg ccacccgggc ggggatcgcc ccgaccgtgc
    16201 ccaccgacag cccgcgcccg cgccgggtca gccgctgggc gagcgtcgtc gcggtgtcct
    16261 cgccgcacca gtcgaccgtg accgggccgc gggccagccg gcgcgcctcg ggcacgtggt
    16321 tgtagaagcc gacgagcagc tccacaggat cgtcctgacc cagcagcacg accgcttcac
    16381 gtgtcaccgg ccagcacgtc agccacggca ccactggccc ggcccggtgc gcgccgtagc
    16441 cgacgagcac gtcgatcccg cgcgcggcca tcacggcacg cagcgcgact cgtcgggcct
    16501 ggagctcggc gtcggtgaag gcgggtggga tcgggtcgac ccgaggctcg gtcactgtcg
    16561 gcatccttca ctcggtcggc ctgaggaccc agggcgccag ccggttctcg atgtgcttga
    16621 gccatgagta gagcgcgaag ccggcgcaca tcagcacgat gaccggcacc agagactcgg
    16681 ccatgttgaa tcgccgggag tgccggagga tcaagaagcc cagccccgtc acggcggtgg
    16741 agaactcggc caccaccact gcgatcaacg acctccccac cgccagacgg actcccgtca
    16801 cgatgtaggg cagtgcgccg ggaacaagga tctctcgcca cgttcgcagc tcggacgagc
    16861 agaacgtctg ggcaagctca ctgaagtcgg ccggcactgc tcgcaccccg gcggcggtgt
    16921 tgatcgtcac cgggaagaac gcaaacatcg cgacgatcac gaccttggcc tggaagccga
    16981 agccgagcac gacgatcagg atcgggatca cggccaccag cggggtggcg aacagcgccg
    17041 agacgtaggg gtcgaggatg acctcgacgg tgcggaagcg accgatgagg atcccgatca
    17101 ccagcccggc gaacgtgccc agagcgaggc cggtcacgaa caccagccca ctggagagcg
    17161 cggcaccggg gagctggccg tcgagggtca tggagacggt ggcctgtgcg acctcgatcg
    17221 gccccacgat cagcgggctc tcctcgatca ccgcgaagag ctgccagccc agtgggatga
    17281 gcagcagccc ggtcaaccaa aggagccgtc gcagacccgc ttcggacatg ccctgcccag
    17341 ggtgggtggg ggcgtccttg ccgactcggc tgcgagcgag ggtcatcgcc cgccctccca
    17401 cggcgagagc cggcgctgca gcatcttgag ccccgcggcg accagcgagc cggccagtgc
    17461 caggaccagc accggcccca gcgcgccggc gatgtcaaac cggttggagt agagaatgat
    17521 caggtagccg aacccactgg caccggcata gaagtccgcc aggaccgctc cgaccagagc
    17581 gtggataagc ccgatccgca gcccgctcac gatcgccggc agcgcgcccg gcacgaggat
    17641 gtcgcgccag agcgcccagc ccttgccgca gaaggcttgc gagagctcaa gcaggtcggg
    17701 gtcgacagtg cgtacgccgg ccgcggtgtt gatgatgacc gtgaggaccg tgaagaggaa
    17761 gacgatgagg atcttggccg tgtagttgaa ccccaccacg acgaggatga acgggatcag
    17821 ggccactagc ggcgtcgtgt agaaaatgtt gagcggagcc tcggtcatcc agtagagcca
    17881 tcgcacccgg ccgatgagga gcccgagtgg cacgccgagg ccgacgccga ggccggctcc
    17941 cacgaccagg atgtaggcgg tcggacccag ataggtcgcg aactcgccgc tggcgatcga
    18001 ttcggcgagt cgggcgatga tctcgtgcgg ggcagccagg aacaggcttc ccgtcatcat
    18061 ggtcgcgaga taccaggccg cgagcagggc cgccgcaaat cccagccgca gagccagcac
    18121 ccgccgtggc gacagccgct gatcagtcac ccgtgacgcc gccgatcgcg tcgggggcga
    18181 gcgtcttctg ccgcagcagc gaccacatct gcgcccggag ctcggcgaag cgcggggaga
    18241 tgcgcatcga tgccgggtcg cggggacgcc cgaaggggat cgggatgtcc tcgacgatcc
    18301 gacccggcct cgactgcatc acgatgacgc ggtccgagag cacgatcgct tcgtcgatcg
    18361 agtgtgtgac gaagacgacc gtcttgcggt cggtcgacca gatccgcatg agctcgtcct
    18421 gcatgagctc gcgggtctgc gcgtcgagcg caccgaacgg ttcgtccatc agcagcgtgg
    18481 ccgggccgat cgccagggcc cgggcaagcc ccacccgttg ctgcatcccg cccgagagct
    18541 gatagggata cttcttctcg ttgccctcga gcccgaccgt cttgaggaag ggcagggcct
    18601 gcgcgcgggc cgcagtcttg ctctgcccgc gcagccgcag accataggcg acgttgtcct
    18661 cgaccgtgcg ccatggcagc agacggaagt tctggaagac catggcggtg tccagtcggg
    18721 gccgggtgct cggcgtcccg ccgaccatga ccgagccgga gctggcttgc tcaatgccgg
    18781 cgaggatgcg cagcagggtg gtcttgccgc atccgctggg cccgaggatc gagacgaact
    18841 cgttgtcgtc gacgtgcagg tcgatggggt cgaggacgtg caggtcacca aagtccttgg
    18901 ttatggcatc aacgtcgagg gccgtcaccg tttgccgaac ctccccgggt gatcactggt
    18961 gtgtggccat cacctggggg ccaccacgcg gttgctagga ctgctcgtcg atgagtgcca
    19021 gcgctgcttc gtagatgctg gtgtcgacga cgtcgtcata ggccggcttc tgtgactcct
    19081 ggatgttgcc cagcttgacc tgctggtctg cggtgtaggc caccatgtct tgcgggtagc
    19141 cgttggcctc cggccagagg ccgtccttga ggaaggtgtc gtaggtgtca gacaggatgt
    19201 cgcgagagag cccggtgacc tcttctgccg tgtcgagcac cgcgtccttg ttggaccggt
    19261 cgctcatgaa ctccttggcc ttgatgttgg ccgcggtgaa ggcgatgatc tcgtcgcgct
    19321 tcttgtcgag gatctcctgc ggggcggcga agccggcgta gtgccactgg ggcaccacct
    19381 cccagaggtt cgcgaggacc ttgaggccgg cgttggggaa ctgctccatg acaccggctg
    19441 cctcatcgat gtgcagcacg ccggagaccg actggccctg cgccatggcc tggccctcgg
    19501 cgcccgggaa gttgccgtat ttcacgtcgt tcttcgtgag gccgcatgag ctgtagaact
    19561 gctgggtcag cacctcggcg aacccgccga cctcgtcgac cgcgagcacc cgacccttga
    19621 ggtcggcaca ggaggagatg tccttggtga ccaccatctg gacatcgagc ttgggcgagt
    19681 aggtgtagaa gcccttgacg tgcgaaccct gcgccaccgc attgatgagc gtcggggtgg
    19741 gcacggccgc gatgtcggct gagccggcct ccgcgccacg cagagccgtg acacctgagt
    19801 cgaggctgac gatcttcacg gagagcccga agtcgtcgta gaagccctgg tcttgggcca
    19861 cccagggcat gatgtgaacg aagttggggg gaatcacggg catgctcagg ctgagcgact
    19921 tcagctcgcc ctcagacgcc gtaccgctgt cgtcaccaga gcacccggtc atgagcacga
    19981 gagccgcgac ggtcagaccg atgagcaggt gctttgagcc aagagactgc ctcacgccga
    20041 gccccctcgt gagcgacggc cctgcaaccg tttgtggcat ggcctgcaag cgtttgtagc
    20101 agccgggtca atgcatgtca atcattgttt gagatatcgc catgatgagg gtttttattg
    20161 caatcagggg catttgtgcc atgtggggca cggcggttcg gccccgccgc ggggctgaag
    20221 cgctgatcag cgggtgggcc caaccgtcgg gcgttgcagg cattcccaga ccttgcgcgc
    20281 ggtcagcggc atggtcagtc ccgtcgtgtc atagtcgcgc agcgcgtcgc agaccgcatt
    20341 gacgagcgcc ggcggggtgc cgatgcagcc ggactcgccg gcgcccttgg tgcccagcgg
    20401 cacgttgggg ttgggggtga ccatctcgac cagccgcagg tcgggcatct ccgcggcggt
    20461 cggcacggtg tagtcgagca gcgtcgtggt cagcggctgg cccgactcgt cgtagacgat
    20521 gccctcatag agcgcctgtc ccagaccttg ggcgatcgac ccgacggtct gcccggacac
    20581 gaccatcggg ttgacgacca ccccgacgtc gtcgaccgcg accagccgca gcaccgcgac
    20641 ctcgccggtg cgggtgtcca cttcgacgac cgcggcgtag gcaccgaacg ggaacgcctg
    20701 cggcggtccg aacacctgct cctccgcgag gtcggggacc gcggcggcca gctcggccag
    20761 gctggtggag cggccgtcgg tggtggtgaa gaccccgtcg gcgtcggcca cgtcggccac
    20821 cgcggcgtcg agccgttggg ccgccgcctc ccgcagccgc tcccgcagcg ccaccgcggc
    20881 gagataggcc gcgttgccgc cgacctgcat cgaccggctg gcgaacgcgc cggtcccctc
    20941 ggcgacctcg gcagtgtcgc cgagacggac gcgtatggcg tccggggaga cacccagccc
    21001 gtcggcgacc acctgggcga acggcgcgag gtgaccctgg ccctgcgagg cggtgccgat
    21061 ccgggccgtc acggtgccgt cggcaccgac ctcgacccgg gcgtactcgg tgctgccgcc
    21121 cagcccgccg gagcgttcca cccaggaggc cagcccgatc ccgacctgcc ggcgctcacc
    21181 gctggtccgc cgccgctgct gctcggcccg ccagccggcg tagtcgagcg cgtccatggc
    21241 ggcgtgcagc gcgtcgtgat ggcgcgcgct gtcatagacc gcgccggtcg gcgagcggta
    21301 gggcagctcg tcggcggtca ggaggttggt ctcgcgcagc cgggccgggt ccaggccgag
    21361 ccggtcggcg gtgaggtcga cgagccgctc gagcgcgaac gccgcctccg gacgacccgc
    21421 gccccggtag gacgcggtgg gcgtggtcgt ggtcacgacg ctgtgcgcga ggacatggat
    21481 ctgcgggatc cggtagggcc cgctcatcac ccaggcgctg agctgcggga tgaagtcgcc
    21541 ggtgtccgga taggcgccca cgtcggccca gatccgcgcc tccaacgcct ggaaccgacc
    21601 gtcacggtcg agcccgagcc gcaggtgggt ggtctggccg cggccgtggg tggccgcgac
    21661 gaaggcttcg tagcgatcct cgatccagcg caccggccgg cccagccgca tcgccgctgc
    21721 ggccacgacg aggtattcgg cgtagacctg gctcttggcc ccgaacgcgc cacccaccga
    21781 cgggaccgcg acccgcactg tggccgggtc cagcccaaag ctgcgggcca gtgcggcgcg
    21841 cagccggtgc ggggcctggt gcgagcacca cacggtgagc ccgccgttgg gctcggggat
    21901 gaccaggatc gcccgggcct ccagcgacat cggcgagacc cgctggttgg tcacccaggt
    21961 ctcgacgacg accgggctgg cctcgaccgc cggcgtgcac ggcgcgccca gctcgcgggt
    22021 gctagagacg ttggagacgc cgtcgaagag ctggggggcg tcggcctcgg ccgcggcccg
    22081 ggcgtcgacc accgccggca gcgggtcgat gtcgacctcg acgagctcgg cggcgtcctc
    22141 ggccgcatag cggcccgccg ccacgacgac ggccaccggt tccccgacga accggacccg
    22201 gtcacgaacc agggccggtc ggcgcatcgc ggcgggctgc tggctgcgcg cggccggcgg
    22261 cacgccgggc aggtcaggca gctcggccgc cgaccagacc gcggccaccc cggccacccg
    22321 ggcggcctgc tcggtgcgta cggcgaccag gtggccgtgg gcggtctggc tgcgcacgaa
    22381 gacggcgtcg agcgcctcgg gcgcggcgat gtcggcgacg aacggcgcgg cgccccggac
    22441 caggtcgtgg tcctccgggc ggggcagaga ctggccgacc caccgccagg tgcggcgggc
    22501 cggacgctcg agaaccgtca cgtcgtcctc cgtgctcgcg gccgccggca aggccttggc
    22561 cgggtgctcg accggatagt agattccgcc gcaatgaaac cgcaatcgtt tgaatatgcg
    22621 gcgccggcca ccctcgccga ggcctgcgac gtgctggcga gccgggccga ggaggccaag
    22681 gtgctcgccg gcgggcagag cctggtgccg atgatggcgt tgcggttggc ccggcccgag
    22741 ctggtcgtcg acctcaacgg ggtggccggc ctcgacgagc tgtcggtcgg cgaggacggg
    22801 ctgcggctgg gcgcgttggt gcggcaccgg cggctggagc ggggcccggt cgacgggccg
    22861 ttgacgtcgc tgctggcccg ggccgcccgt cacatcgggc acccgccgat ccgggcccgg
    22921 ggcacggtct gcggcagcct ggcgcacgcc gacccggctg ccgagtggtg cctggtgggc
    22981 gtggccaccg acgcgcaggt cggcgtggtc agcgccggcg gccggcgcga gctcacggtc
    23041 gacgcgctgc tggacggccc gttctccacc acgctcgagc ccgacgaggt gatcgccgac
    23101 gtgacgttcc ccgcctggcg cccgggccgg cacggggccg ggctggccga gcgctcgcgc
    23161 acggcgggcg ggttcgccga gctggccgcg tgcgccgtgc tcgaggtggc cgacgcgacg
    23221 gtggccgccg ccgcggtggc cgtggtcgga gccgcgggcc ggccggtgcg gctgcccggc
    23281 accgaggccg ccctcgtcgg cagcccggcc ggtccgggcg gctcgtgggc cggcaccgtc
    23341 gcgcgggccg gcgcgctcgc ggtcacggag gtgcccgacc gcggcgacgg cggctatgcc
    23401 gccgccgtgc tcggcgcgct ggtcgaggag gcgctcaccc aggcgcagga ggaggtgcgg
    23461 cgatggacgt ccgactgacc gtcgacgggc gcgaccacct ggtgcaggtg gaggcgcgca
    23521 ccacgttggc cgacgcgctg cgcgaggagt gcggcgtgac cgccgtacac ctggggtgtg
    23581 agcagggctc gtgcggagcc tgcaccgtgg tgctcgacgg cgacgcggtg cgttcctgcc
    23641 tggtcttcgc cgtgcaggcc gatggcaagc gggtgggcac cgcggccggc ctggccgacg
    23701 gcgagcacgc ccaccccgtg cagcaggcgc tgtccgagca ccacggcctg cagtgcggct
    23761 tctgcacgcc ggggttcgcg gtgctgctgg cccatgccct cgacgagcgg cccgacctgg
    23821 acgacgacga gctggtcgag ctggtctcgt ccaacctgtg ccgctgcacc ggctatcgcg
    23881 gcatcctggc cgccgcgcgc agcgtgcggg accagcgggc cggctcaggc cttggcgcgg
    23941 cgggtccgac gggcggctga ggccttgcgc cgcggtgggg cggtcgactc gcggcgcacg
    24001 agcgtgagcg gcagcagcac cgagcgggcc gagcgggtcg ggtcgcgcag ctcttcgagc
    24061 agcatgcgcg ccgcctccgc cccgatgtcg tagtgcggga tgtggatggt cgtcagcggc
    24121 gggcgcagct tgtcgaagaa tggcgtgtcg ttgaacccca ccacgctgat gtcctcgggg
    24181 cagcgcagcc cgcgttcttg gagcacgtcg tagacgccca cggcctgcat gtcgttgccg
    24241 gcgaacacgg cggtgaactc gacgccctcg tcgaggatct cccgcatcgc ccgggcgccc
    24301 tcggcgacgg agaaggccga gcaccgcacg atcagtcgcg ggtcgtcgtc gaggccgtgc
    24361 tcgtgcaccg cctgccggaa ggaccgcagc cgggtcaccc cggtggagac ggtctgcggg
    24421 ccggcgaggt ggacgatgcg ccggtggccg agctcggcga ggtggctcac ggcgagcgcg
    24481 acaccggtcg cgtcgtctga ggtcacggtc ggtacgtcgg tgcgggcgag ccgccggttg
    24541 atgaggacca tcggcaggtc ctcggccgcg agcttgtcga gcaacgggtc ctcgagcagc
    24601 gcggtcgcga tgatgaggcc ctcgacgtgg cgggagcgca tcgactcgac gtgggccgcc
    24661 gcgcggtcgg ggtcgttgtc ggtgttggcc agccaggcgt tgtagccggc cggcgcgagg
    24721 acgtcttcga tgccacgcac gatcggcggg aacagcgggt tggtcaggtc gggaatgatc
    24781 atcccgatgc tcgtcgaccg cgccgtcttg aggctgcggg cgatcgggtt ggggcggtag
    24841 cccagcgccg ccgccacgcg agcaacccgc tcggccgtcg cgtcgttgac gagcttgcgg
    24901 gtgctggggt tgagcgcgcg cgagacggtg gccgggtgca ccccggcctc ccgtgccaca
    24961 tcacgcaggg tgggggccct catcatgcgg aggatatcgg gtagccgggc cccagcgtgc
    25021 aagcggttgc agtcttttgc acgatggtga cggtcgtcgc cgtcacgatt gtcccgcgcg
    25081 cagctgctcg gccgggacgg gcgaccaccg gtgccggctg atcgcggcga ccagcccgag
    25141 cgggacggtc gccgccagcg cgaccaggaa gatcagcgcc catcgtccct ggtcggcgct
    25201 cgagccgccc agcgcggagc tgaccgcgct gccggcgaac aatgccccag caaacaatga
    25261 gatcaccgtg gcgcgggcgc tgggcagcac catggtggcc caggtctgca gcgtcgagtg
    25321 catgaatgcc cacccgatgc cgagcagcac gcacaccgcc agcaccggcc ccggccgggt
    25381 ggagagcgcc gcgagcgcgc aggccgctgc ggtcgccagc gcgccgacca cgatgagccg
    25441 agccggcggc atccgtgccg agaggcggcc gaccgcggtg gccgccacga gcacggccag
    25501 cccatagatg cccaccaccg cgccggccac cgccgggctc accccgccgt cctcgaccgc
    25561 agcgggcagg taggtgatca ccccgatgat gatggctccc tcggcgaagg ccagcccgat
    25621 gacgagcacg gaggcccggt gccgtaggac gtcacgcagc ggggccagga tcggccggcc
    25681 cattctggtg cgcggcggcc ggggcagggc ccgcagcgcg aaggcgagcc ccagccccag
    25741 cagcccggtg gccacaaaca tccagcgcca gccgatggtg gccgcgagca ggccggcgcc
    25801 ggcggtcgcc accgcggtgc ccagcgccga tcccgacagg aggccggtca cctcgtgctg
    25861 gcgtcgtacg ccgggcaccg tgtcgccgac gtagaccagg gcggccggga acgctgcgct
    25921 gaagcagccg ccggcgagcg tgcgggagac cagcagccag ctcacccccg gtgaggcggc
    25981 cgaggcgagc gagaagacgc cggcggccag caaggtgatc cgcatggtgc ggaccagccc
    26041 gatccggtcg gaggcgatgc cccagagcgg ctgggtcacg ccgtaggcca ggaagtaggc
    26101 gctcgcggcg gcgacgatca ccgtgagcgg g
//
LOCUS       DATIBB010000142         6664 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_1137532, whole genome shotgun sequence.
ACCESSION   DATIBB010000142 DATIBB010000000
VERSION     DATIBB010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 6664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6664
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1137532"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            241..810
                     /locus_tag="VLC50_05485"
     CDS             241..810
                     /locus_tag="VLC50_05485"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HSF26953.1"
                     /translation="MTYGGFEPYGGFADTDLGPAAAPWRPRWQASLWDLLAIAGWLGV
                     LTVAGLLLRPLLTRLAEAPMPAVDALVLAATVLPVWTYLTVTEAGPAHASWGKRRVGL
                     QVVCSHDGAGPGFARSAIRNAVKLAPWQLAHFAVVRLAAGDTSATVAVTDVLSLLIPV
                     VSLVMAWRDGRRRSLHDRIAGTTVVTRAP"
     gene            complement(823..1080)
                     /locus_tag="VLC50_05490"
     CDS             complement(823..1080)
                     /locus_tag="VLC50_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014738259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF26954.1"
                     /translation="MAICEVCGNDYRLSFEVHAQGAVHVFDSFECAVHRMAPRCEHCG
                     CTVIGHGVEADGHIFCCASCARQVGLVSASSLADHAGVDPR"
     gene            complement(1185..2960)
                     /locus_tag="VLC50_05495"
     CDS             complement(1185..2960)
                     /locus_tag="VLC50_05495"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019811201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HSF26955.1"
                     /translation="MLLRMSSLFLRTLREDPADAEVPSHKLLVRAGYVRRVAPGIYSW
                     LPLGKIMLENVSRVVREEMDRIGAQEVLFPALLPRDYYEATGRWTEYGPNLFRLKDRK
                     GVDYLLGPTHEEMFTDMVKGEYSSYKDLPVMLYQIQTKYRDEARPRAGILRGREFVMK
                     DSYSFDLDEAGLQKSYDLHREAYVRLFERLGMRYVIVSAMSGAMGGSASEEFLAPAEY
                     GEDTYVACRACGYAANVEAVRTPAPEASPIADQPASQLLDTPDTPTIGTLVDRLNELE
                     LGRTFTAADTLKNVVFKTRAPGATEWTPLVVGLPGDREVDMKRLEAALEPAEVAVMEA
                     EDFARFPGLVRGYIGPQVLAGLGIRYLVDPRVVTGSAWVTGANVPDKHAANVVAGRDF
                     VADGTIEAADVRAGDPCPQCGAELSIDRGIEIGHIFQLGYKYADAAQLDVLGPDGKPI
                     RVIMGSYGVGVSRAIASVAEQSHDDKGLVWPREIAPADVHIVATGKDDAVFETADDLA
                     RRFEGHGLRVLLDDRRGVSPGVKFNDSELIGVPTIVVVGRGLAEGLIEVKDRRSGERE
                     NVPLEQAVARLVEICGQDRPVPHSA"
     gene            complement(3099..4214)
                     /locus_tag="VLC50_05500"
     CDS             complement(3099..4214)
                     /locus_tag="VLC50_05500"
                     /inference="COORDINATES: protein motif:HMM:NF024734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HSF26956.1"
                     /translation="MPVRPPFVGSARLSTAGAPAGLVHKRPAARSSYAGTGITVTRMS
                     PLVPELESLARTQGGVFLARQAIAAGISHRELTRRCRHGELVHLRRGAYAMASATDGA
                     LTEPVRHKLLSRSALLVLLGEAWLSHQSAAVFHGLDQHRPDLSVVHVTRPGISSARVE
                     AGVAHHDGGLPEHHRTIVDGVPVVSLGRTVIDVARSHSFDQAMVAADNALHAGLARAD
                     LEEALDFCRDWPGAAQAGSVVAFADGRTESAGESLTRCVLLRGGLAPDELQLVLPGGN
                     GLRVDFAWTRWRVVLEFDGKVKYGRSLAPDGDASEVAWRERRRELAIERLGWVVVRVT
                     WAEVVFHPQVVIERVREAIALAHARGLVGARGLARIR"
     gene            complement(4343..5191)
                     /locus_tag="VLC50_05505"
     CDS             complement(4343..5191)
                     /locus_tag="VLC50_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006139499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4081 domain-containing protein"
                     /protein_id="HSF26957.1"
                     /translation="MLRTPSLRVLGPADRDELLALLDREPVADVFVASRVREAGLEPW
                     RLGAEVWGWYDRGQLTAACYSGANLVPVQAGSDEHALRAFADRARRQGRRCSSIVGPA
                     AMVAQLWARLEPHWGRARAVRANQPLMLLDHAPDVAPDPAVRLVGVDEIDVLLPASVA
                     MFTEEVGISPIASDGGRLYRARVAELVSGRRALARIEDGHVVFKAEIGSATPLACQVQ
                     GVWVEPSMRGRGLAVGGVAAVAAHAMAHFAPAVGLYVNDYNTAAIAAYRHVGFRQVGT
                     FATVLF"
     gene            5303..6250
                     /locus_tag="VLC50_05510"
     CDS             5303..6250
                     /locus_tag="VLC50_05510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF26958.1"
                     /translation="MGAETPSWSQMLGRMAGFGARTATGALRFRDPGIPADADAPERA
                     EPREGTCRFWYAAPDRWRVEDERGVWHVQDDHWINLRDDQGRMQRLPREGTIWGFALG
                     HPQYLFGSSNDRLERFTRDNDFSLPMGPATAVTVAGRSAWEFVLAPPPHKPHPLRVAF
                     DDATGVVVRMAVPELSFLIEITELAVNLELDAQTFTWTGPVATDQYDDIVGQARAREW
                     LEQQRVPVPRWWPDGLGYSPASGDPETGALSVELSAPGEPMLARWPIGGAQPPYWDLR
                     TEGRHVHTWSDGTWQWALAVTAPLTDDDLARVIASIPAK"
     gene            complement(6287..>6664)
                     /locus_tag="VLC50_05515"
     CDS             complement(6287..>6664)
                     /locus_tag="VLC50_05515"
                     /EC_number="1.17.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019876484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HSF26959.1"
                     /translation="VKILESLGMRERGLEIVSCPSCGRAQVDVYTLAERVTAGLEGLE
                     VPLRVAVMGCVVNGPGEAREADLGVASGNGKGQIFVKGQVIKTVPESEIVETLIEEAM
                     RLAEEMAAAGETGEGTAPVVTVS"
BASE COUNT          977 a         2433 c         2349 g          905 t
ORIGIN      
        1 gaggacccgc cgcggacaca cacctggatg gtcggctacc agggtgagat cgcgttcgcg
       61 gctttcgtcg aggacggcgc cagtggggct tcgacctcgg ccccgatcgt gcgcaggttt
      121 ctcaccgcac tggcccgctg acccggcggg atgaccgcgc cacgcgggcg tatcggaggg
      181 cgcgcgacgc ctgcaccgac cctgcccgcg ctgccgcggc ggtcgtgatt gcatcctctc
      241 gtgacctatg ggggatttga gccgtacggc ggcttcgcgg acactgacct cgggcccgct
      301 gcggcgccgt ggcgccctcg ctggcaggcg agcctgtggg acctgctcgc gatcgctggc
      361 tggctcggcg tgctcaccgt ggccggcctg ctcctgcgtc cgttgctgac ccggctggcc
      421 gaggcaccga tgccggcggt ggacgcgctc gtgctcgccg ccacagtgct gccggtgtgg
      481 acctacctca cagtgaccga ggccgggcca gcgcacgcga gctggggcaa gcgccgggtc
      541 gggctgcagg tggtctgctc gcacgacggg gctggccccg gctttgctcg cagcgcgatc
      601 cgcaacgcgg tcaagctggc gccctggcag ctggcccatt tcgccgtcgt ccgactggcc
      661 gccggcgaca cctcggccac cgtcgcggtg accgacgtgc tgtcgctgct gatccccgtg
      721 gtcagcctgg tgatggcctg gcgtgacggc cgccgccgca gcctgcacga ccggatcgcc
      781 ggcacgacgg tggtcacccg cgcgccgtga cggcggcggt cgctaccgcg ggtcgacgcc
      841 ggcgtggtcg gccaggctgg acgcgctgac caggcccacc tgccgcgcac agctggcgca
      901 gcagaagatg tgcccgtcgg cctccacgcc gtgcccgatc acggtgcagc cacagtgctc
      961 gcagcgcggt gccatccgat gcacggcgca ctcgaagctg tcgaacacgt gcaccgcgcc
     1021 ctgggcgtgc acctcaaagc tgagccggta gtcgttgccg cagacctcgc agatcgccat
     1081 gctgccccgc ccctcgttgg ccgccgccat gatcgccgta cggcgaatgg ctgcctggcc
     1141 tcgttgttcg cacgatgagg cggccgggcg cggctggcaa ccttttaggc cgaatgcgga
     1201 accggtcggt cctgaccgca gatctcgacg aggcgggcca ccgcctgctc gagcggcacg
     1261 ttctcccgct cgccggagcg ccggtccttg acctcgatga ggccctcggc cagcccccga
     1321 ccgaccacga cgatggtcgg tacgccgatc agctcggagt cgttgaactt cacgcccggc
     1381 gagacgccgc gccggtcgtc gagcagcacc cgcagcccgt ggccctcgaa ccgccgagcc
     1441 aggtcgtcgg cggtctcgaa gaccgcgtcg tccttgccgg tcgccacgat gtgcacgtcg
     1501 gccggggcga tctcgcgcgg ccagaccagc cccttgtcgt cgtggctctg ctcggcgacc
     1561 gaggcgatgg cgcgcgagac accgacgccg tacgacccca tgatcacccg gatcggcttg
     1621 ccgtcggggc ccagcacgtc gagctgcgcg gcgtcggcgt acttgtagcc gagctggaag
     1681 atgtgcccga tctcgatgcc ccggtcgatc gacagctcgg caccgcactg agggcacggg
     1741 tcaccggccc gtacgtcggc cgcctcgatg gtgccgtcgg cgacgaagtc gcgcccggcg
     1801 acgacgttgg ccgcgtgctt gtcgggcacg ttggccccgg tcacccaggc gctgccggtc
     1861 accacccgcg ggtcgaccag gtagcggatg cccagcccgg ccagcacctg cggcccgatg
     1921 tagccgcgga ccagccccgg gaagcgggcg aagtcctcgg cctccatcac tgcgacctcg
     1981 gccggctcga gcgccgcctc cagccgtttc atgtcgacct cacggtcacc gggcagcccg
     2041 acgaccaacg gggtccactc ggtggcgccc ggcgcgcggg tcttgaagac gacgttcttg
     2101 agcgtgtcgg cggccgtgaa cgtgcggccc agctccagct cgttgagccg gtcgaccagc
     2161 gtcccgatgg tcggggtgtc gggggtgtcg agcagctggc tggccggctg gtcggcgatc
     2221 gggctggcct ccggcgcggg agtgcgcacc gcctccacgt tggcggcgta accgcaggcc
     2281 cggcaggcga cgtacgtgtc ctcgccgtac tccgccggag ccaggaactc ctcgctcgcg
     2341 ctgccgccca tcgccccgga catcgccgag acgatgacgt agcgcatgcc caggcgctcg
     2401 aagagccgga cgtaggcctc ccggtgcagg tcatagctct tctgcaggcc ggcctcgtcg
     2461 aggtcgaagg agtaggagtc cttcatgacg aactcgcgcc cgcgcaggat gccggcgcgg
     2521 ggccgcgcct cgtcgcggta cttggtctgg atctggtaga gcatcaccgg caggtccttg
     2581 taggacgagt actcgccctt gaccatgtcg gtgaacatct cttcgtgggt gggcccgagc
     2641 aagtagtcga cgcccttgcg gtccttgagt cggaacaggt tggggccgta ctcggtccac
     2701 cgcccggtgg cctcgtagta gtcacgtggc agcagcgccg ggaacagcac ctcctgggcg
     2761 ccgatccggt ccatctcctc gcgcaccacc cgggagacgt tctccagcat gatcttgcca
     2821 agcggcagcc aggagtagat gcccggcgcg acccggcgga cgtagccggc gcgcacgagg
     2881 agcttgtggc tgggcacctc ggcgtcggct gggtcctcgc gaagggtacg caggaacagc
     2941 gacgacatcc gcagcagcac ggccgggctc cttcagaggg tacgacgagc ggtgcagcga
     3001 ggatatcggc cgccctaccc ctgcgaatga ccacgccaag tgggcgtgcg tgcacttggc
     3061 gaggcgtgca cgccttgcca agtgcaacaa cgccttgcct agcggatccg ggcgaggccg
     3121 cgcgcgccga ccagaccccg cgcatgcgcc agcgcgatcg cctcacgcac ccgctcgatc
     3181 accacctgcg ggtggaacac aacctcggcc caggtgacgc gtacgacgac ccagcccagc
     3241 cgctcgatcg ctagctcgcg acgacgctct cgccaggcga cctcggaggc gtccccgtcc
     3301 ggagccagcg atcggccgta cttgaccttg ccgtcgaact cgagcacgac tctccaccga
     3361 gtccaggcga agtcgacccg cagcccgttc ccacccggca ggacgagctg gagctcatcg
     3421 ggcgcgagcc caccccgcaa gagcacgcac cgggtcagcg actcgccggc cgactcggtg
     3481 cgaccgtcgg cgaaggccac gacactcccc gcctgggcgg cacccggcca gtcgcggcag
     3541 aagtcgagcg cctcctcgag gtccgcgcgg gccagacccg cgtgcaacgc gttgtcagcg
     3601 gccaccatcg cttgatcgaa cgagtgcgag cgtgcgacgt cgatcacggt gcgacccagc
     3661 gagaccaccg ggacgccgtc gacgatcgtg cggtggtgct ccggcaggcc accgtcgtgg
     3721 tgagcgacac cggcttcgac ccgcgccgag gagatgcccg gacgagtcac gtggacgacc
     3781 gacaggtcgg gacggtgctg gtcgaggcca tggaagacgg ctgccgactg gtggctgagc
     3841 caggcctcgc cgagcagcac gagcagggcc gagcgggaca gcagcttgtg ccgcaccggc
     3901 tcggtcagcg cgccatcggt cgcgctcgcc atcgcgtagg caccccgccg cagatgcacc
     3961 agctcgccat ggcggcaccg ccgggtgagc tcgcgatggc tgatcccggc ggcgatcgcc
     4021 tgccgggcga ggaacacgcc accctgcgtg cgcgcaagcg actccagctc gggaacaagt
     4081 gggctcatgc gggtcacggt gatcccggtg ccggcgtacg acgacctcgc ggcgggccgc
     4141 ttgtggacga ggccagcggg agcgccggct gtggacaacc gggccgagcc gacgaacggc
     4201 gggcgcacag gcatcgtcgt acggccgaga accgttcact aggcaaggcg ttgtggcact
     4261 aggcgaggcg tgcacgcctg gccaagcgca cggacgccca cttggcgtgg tcattccccc
     4321 gggcagcgag agcgggggcg ggtcagaaga ggacggtcgc gaaggtgccg acctggcgga
     4381 agccgacgtg gcggtaggcc gcgatggccg cggtgttgta gtcgttgacg tagagcccca
     4441 ccgccggcgc gaagtgagcc atggcgtggg cggcgaccgc tgccacgccg ccgaccgcca
     4501 gcccgcgccc gcgcatcgac ggctcgaccc agacgccctg gacctggcag gcgagcgggg
     4561 tcgccgagcc gatctcggcc ttgaacacca cgtgcccgtc ctcgatccgg gccagcgcac
     4621 gccggccgga caccagctcg gccacccggg cccggtaaag ccggcccccg tcggatgcga
     4681 tcggcgagat gccgacctcc tcggtgaaca tggcgaccga ggcgggcagc agcacgtcga
     4741 tctcgtcaac acccaccagg cgtacggcgg ggtcgggcgc gacgtcgggg gcgtggtcga
     4801 gcagcatcag cggctggttg gcgcgcaccg cccgggcccg gccccagtgc ggctccagcc
     4861 gggcccacag ctgcgccacc atggccgccg ggcccacgat cgacgagcag cgccggccct
     4921 gtcggcgcgc gcgatcggca aacgcgcgca gggcgtgctc gtcggagccg gcctgcaccg
     4981 gcacgaggtt ggcgccggag tagcaggcgg cggtgagctg cccacggtcg taccaacccc
     5041 agacctcggc gccgagccgc cacggctcga gcccggcctc acgcacccgc gaggccacga
     5101 agacgtcggc caccggctcg cgatcgagca aggccagcag ctcgtcgcgg tcagcagggc
     5161 ccagcacgcg caatgagggc gtacgcagca cgccaccctc ctccatacct tgaccgctcg
     5221 cgcacctgac gctaccggag cgcgcccgag cctgccgacg cggaaccgcc ggtccggacg
     5281 atctcccagt agcgtgccgc ccatgggggc agagactcca tcgtggtcac agatgctcgg
     5341 gcggatggcc ggcttcggtg cccgcaccgc caccggcgcg ctgcgcttcc gcgaccctgg
     5401 catccccgct gacgcggacg cccccgagcg tgccgaaccg cgcgagggca cctgccggtt
     5461 ctggtacgcc gcgcccgacc ggtggcgggt cgaggacgag cgtggcgtct ggcacgtgca
     5521 ggacgaccac tggatcaacc tgcgcgacga tcagggtcgc atgcagcgcc tgccgcgcga
     5581 gggcacgatc tggggcttcg cgttggggca cccgcaatat ctgttcggca gcagcaacga
     5641 ccgtctcgaa cgcttcactc gggacaacga cttctcgctg ccgatggggc cggccaccgc
     5701 ggtcaccgtg gctggccgct cggcctggga gttcgtcctg gcgccgccgc cgcacaaacc
     5761 gcacccgctg cgggtcgcgt tcgacgacgc gaccggcgtg gtcgtgcgga tggcggtgcc
     5821 ggagctgagc ttcctcatcg agatcaccga gctcgcggtc aacctggagc tcgacgcgca
     5881 gacgttcacc tggaccggcc cggtggcaac ggatcagtac gacgacatcg tcggccaggc
     5941 ccgggcgcgg gagtggctgg agcagcagcg ggttccggtg ccccgctggt ggccggacgg
     6001 gctgggctac tcgccagcca gcggcgaccc ggagaccggg gcgctcagcg tcgagctgtc
     6061 ggcccctggt gagccgatgc tggcgcgctg gccgatcggc ggggcacagc cgccgtactg
     6121 ggatctgcgc accgagggcc gacacgtgca cacctggtcg gacgggacat ggcagtgggc
     6181 gctagccgtc accgcgccgt tgaccgacga cgacctggca cgagtgatcg cctcgatccc
     6241 cgccaagtga gccccgccat tcgcgcatga tcagtgcagg agggggtcag ctgacggtga
     6301 cgaccggggc ggtgccctca ccggtctcgc cggcggcggc catctcctcg gccaggcgca
     6361 tggcctcctc gatcaaggtc tcgacgatct ccgactcggg caccgtcttg atgacctggc
     6421 ccttgacgaa gatctgaccc ttgccgttgc ccgacgcgac gccgaggtcg gcctcgcggg
     6481 cctcccccgg gccgttgacg acgcagccca tcaccgcgac ccgcagcggc acctcgaggc
     6541 cctcaagccc ggcggtcacc cgctcggcca gcgtgtagac gtcgacctgg gcccgcccgc
     6601 acgaggggca ggagacgatc tcgagcccgc gctcgcgcat ccccaacgac tcgaggatct
     6661 tgac
//
LOCUS       DATIBB010000143         4350 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_875403, whole genome shotgun sequence.
ACCESSION   DATIBB010000143 DATIBB010000000
VERSION     DATIBB010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 4350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4350
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_875403"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(35..967)
                     /gene="pucL"
                     /locus_tag="VLC50_05520"
     CDS             complement(35..967)
                     /gene="pucL"
                     /locus_tag="VLC50_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016894636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004846 - urate oxidase activity [Evidence
                     IEA];
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urate oxidase"
                     /protein_id="HSF26960.1"
                     /translation="MAIVLGPNQYGKAETHLVRVYRDTDRHEIRDLTVSTSLRGDFTD
                     AHRLGDQAHVLPTDSQKNTVLAFAKERGVGQIEDFALALARHFVDDVEAVETARVDVD
                     EHSWERIEVDGRPHDHAFVRGGAQTRTATAVASGRGEDARVWVVTGLKDLALLKSTGS
                     EFAGFATDRFTTLAETHDRVLATSLTARWRHGSTTLGAAGGPAVDWAGSFAAVRSTLL
                     ATYARVHSLAMQQTLWEMGKAVLELCPDVVEVRLSAPNKHHFLVDLAPFGLDNPGEVF
                     FAADRPYGLIQASALRDDAPDPGPVWAEAPSAFV"
     gene            complement(970..1311)
                     /gene="uraH"
                     /locus_tag="VLC50_05525"
     CDS             complement(970..1311)
                     /gene="uraH"
                     /locus_tag="VLC50_05525"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013151771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HSF26961.1"
                     /translation="MSVVTTHVLDTVRGRPAAGVPVRLEQLIVDGLPGHPLADAVTDE
                     DGRLDELGPDRLDVGTYRLVFDTAAYFKATGQDRFFPEVSVVFAVADPATHVHVPLLL
                     APYAYSTYRGS"
     gene            complement(1308..1829)
                     /gene="uraD"
                     /locus_tag="VLC50_05530"
     CDS             complement(1308..1829)
                     /gene="uraD"
                     /locus_tag="VLC50_05530"
                     /EC_number="4.1.1.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019666165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline
                     decarboxylase"
                     /protein_id="HSF26962.1"
                     /translation="MDEGIGLDGFNALPAEQARTVLLACCAAPRWAERLTRGRPYRTP
                     EQLFEVADEVLADLDDLALLEAVAAHPRIGAPLPAPAGGSQRSADWSSHEQSAVADAD
                     ESTLAALAEGNRRYEQRFGHVYLVAAGGRTADELLALLHTRLANDPATERRVLRGELA
                     AINRARLARVVGP"
     gene            complement(1895..3283)
                     /locus_tag="VLC50_05535"
     CDS             complement(1895..3283)
                     /locus_tag="VLC50_05535"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010695640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HSF26963.1"
                     /translation="MPTDRIETVVNLCKRRGFVYPSGEIYGGTRSAWDYGPLGVELKD
                     NVKRQWWKTMIQSRDDVVGLDSSVILPRDVWVASGHVEAFVDPLVECQSCHRRFREDH
                     LKEEYAERKKRDDADAVAMTDVPCPNCGTKGAWTEPRMFNGLLKTFLGPVEDESGLHF
                     LRPETAQGIFVNFANVMSSARRKPPFGIGQIGKSFRNEITPGNFIFRTREFEQMEMEF
                     FVEPGTDEQWHQYWIDLRTDWYVDLGIARDNLRHYEHPKEKLSHYSKRTVDIEYRFHF
                     AGSEWGELEGIANRTDFDLTTHSKHSGADLTYFDQDKGERWTPYVIEPAAGVGRPMMA
                     FLLEAYAEDEAPNTKGGVDKRVVLRLDPRLAPVKVAVLPLSRNADLSPKARDLAATLR
                     KSWNVDFDDAGAIGRRYRRQDEIGTPFCVTVDFDTLTDQAVTVRERDSMRQERIGLDQ
                     VQAWLGARLPGC"
     gene            complement(3375..3767)
                     /locus_tag="VLC50_05540"
     CDS             complement(3375..3767)
                     /locus_tag="VLC50_05540"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HSF26964.1"
                     /translation="MALHTLVVTRYRVSEGTAASFRDRIRDALEAFAATPGFVDGRVG
                     RSTDEAGLWVLELTFRDVGSYRRALSSYDVKLRAVPVMYDAVDEPSAYEVLEQAGVGG
                     RASSDLAADAGAVNLGEAAAPVVVSDLD"
     gene            3849..4169
                     /locus_tag="VLC50_05545"
     CDS             3849..4169
                     /locus_tag="VLC50_05545"
                     /inference="COORDINATES: protein motif:HMM:NF042772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6703 family protein"
                     /protein_id="HSF26965.1"
                     /translation="MVEQRRRAARPGSMRARVEQASAPALVRLTRLPRLLVPAIFGVL
                     VIVALATPAPIALAATVVVVAFVGWLTYLSWPAITPGGRVFRLVTLGLLVGYLVWLLQ
                     GPAA"
BASE COUNT          578 a         1528 c         1593 g          651 t
ORIGIN      
        1 gggttcgggt gcgaacacgg gtcggctcag gcgctcacac gaacgccgac ggtgcctcgg
       61 cccagacggg gccggggtcg ggggcgtcat cgcgtagggc ggaggcctgg atcagcccgt
      121 atggccggtc ggcggcgaag aagacctcgc ccgggttgtc gagcccgaac ggggccaggt
      181 cgacgaggaa gtgatgcttg ttgggcgccg acaggcgcac ctcgacgacg tccggacaga
      241 gctcgagcac cgccttgccc atctcccaca gggtctgctg catcgcgagg ctgtgcaccc
      301 gcgcataggt ggccagcagg gtcgagcgga ccgcggcgaa cgagccggcc cagtcgactg
      361 ccgggccacc tgccgcgccg agggtcgtgc tgccgtggcg ccagcgcgcg gtcaacgacg
      421 tggcgagcac ccggtcgtgg gtctcggcca gcgtggtgaa gcgatcggtc gcgaagccgg
      481 cgaactccga ccccgtcgac ttgagcagcg ccaggtcctt cagcccggtc accacccaca
      541 cccgggcgtc ctcgccccgg ccgctggcca ccgcggtcgc cgtacgcgtc tgtgctccgc
      601 cccgcacgaa ggcgtggtcg tgcggacgcc cgtccacctc gatgcgctcc caggagtgct
      661 cgtccacgtc gacccgggcc gtctcgaccg cctcgacgtc gtcgacgaag tgccgagcca
      721 gggccagcgc gaagtcctcg atctgcccga ccccgcgctc cttggcgaag gcgagcacgg
      781 tgttcttttg cgagtcggtc ggcagcacgt gggcctggtc accgagccgg tgcgcgtcgg
      841 tgaagtcccc gcgcagcgag gtggacacgg tgaggtcgcg gatctcgtgc cgatcggtgt
      901 cgcggtagac ccgcacgagg tgggtctccg ccttgccgta ctggttgggc ccgagcacga
      961 tcgccatgat cagcttcccc ggtaggtgga gtaggcgtag ggcgcgagca gcagcggcac
     1021 gtgcacgtgc gtggcgggat cggcgacggc aaagaccacc gacacctcgg ggaagaagcg
     1081 gtcctggccc gtcgccttga agtaggccgc cgtgtcgaag accagccggt aggtgccgac
     1141 gtccagccgg tcggggccca gctcgtcgag ccgcccgtcc tcgtcggtca ccgcgtcggc
     1201 cagcgggtgg ccgggcaacc cgtcgacgat cagctgctcc aaccgcaccg gcaccccggc
     1261 cgcgggccgg ccccggacgg tgtcgagcac atgcgtggtg accacgctca tgggccgacc
     1321 acccgcgcca gccgcgcccg gttgatcgcc gcgagctcgc cgcgcaggac acgccgctcg
     1381 gtggccgggt cgttggccag ccgcgtgtgc agcagcgcca gcagctcgtc ggcggtgcgg
     1441 ccaccggccg ccaccaggta gacgtgaccg aaccgctgct catatcgccg gttgccctcg
     1501 gccagggccg ccagcgtcga ctcgtcggcg tccgcgacgg cggactgctc gtggctcgac
     1561 cagtcggccg atcgctgtga gccgccggcc ggggccggca ggggggcccc gatgcgcggg
     1621 tgggcggcca ccgcctcgag cagcgccagg tcgtcgaggt cggccagcac ctcgtcggcg
     1681 acctcgaaga gctgctcggg cgtgcggtag ggccggccgc gggtcagccg ctcggcccac
     1741 cgcggcgccg cgcagcaggc gagcagcacg gtccgggcct gctcggcggg caacgcgttg
     1801 aacccgtcga ggccgatccc ctcgtccacc acgcgccctc cccgtgctgc gccgtggctc
     1861 agttgtaccc gaatccggcg ccggggggag ccaatcagca gccgggcagc cgggccccca
     1921 gccaggcctg cacctggtcg agaccgatcc gctcctggcg catcgagtcg cgctcgcgca
     1981 ccgtcaccgc ctggtcggtg agggtgtcga agtcgacggt gacgcagaac ggcgtgccga
     2041 tctcgtcctg acggcggtag cggcgcccga tcgcgccggc gtcgtcgaag tcgacgttcc
     2101 agctcttgcg cagcgtggcg gccaggtcac gggccttcgg cgagaggtcg gcgttgcgcg
     2161 agagcggcag tacggccacc ttgaccggcg ccagccgcgg gtccagccgc agcacgaccc
     2221 gcttgtccac cccgcccttg gtgttgggcg cctcgtcctc ggcgtaggcc tcgagcagga
     2281 aggccatcat cgggcgcccc acgccggccg ccggctcgat gacgtagggg gtccagcgct
     2341 cgcccttgtc ctggtcgaag taggtcaggt cggcgccgga gtgcttggag tgggtggtca
     2401 ggtcgaagtc ggtgcggttg gcgatgccct cgagctcgcc ccactcgctg ccggcgaagt
     2461 ggaaccggta ctcgatgtcg acggtgcgct tggagtaatg ggagagcttt tccttggggt
     2521 gctcgtagtg gcgcaagttg tcgcgcgcga tgcccaggtc gacgtaccag tcggtgcgca
     2581 ggtcgatcca gtactggtgc cactgctcgt cggtgcccgg ctcgacgaag aactccatct
     2641 ccatctgctc gaactcgcgg gtgcggaaga tgaagttgcc cggggtgatc tcgttgcgga
     2701 agctcttgcc gatctggccg atgccgaacg gcggcttgcg ccgggccgag gacatcacgt
     2761 tggcgaagtt gacgaagatg ccctgggcgg tctcggggcg caggaagtgc aaaccggact
     2821 cgtcctcgac cgggccgagg aacgtcttga gcagcccgtt gaacatccgc ggctcggtcc
     2881 aggccccctt ggtgccgcag ttggggcacg gtacgtcggt catcgcgacc gcgtcggcgt
     2941 cgtcgcgctt cttgcgctcg gcgtactcct ccttgaggtg gtcctcgcgg aagcgccggt
     3001 gacaggactg gcactcgacc aacgggtcaa cgaacgcctc gacgtggccg gaggccaccc
     3061 acacgtcgcg cggcaggatc accgaggagt ccaggccgac cacgtcgtcg cggctctgga
     3121 tcatggtctt ccaccactgc cgcttgacgt tgtccttgag ctccacgccg agtgggccgt
     3181 agtcccaggc cgaccgggtg ccgccgtaga tctcgcccga cgggtagacg aacccgcggc
     3241 gcttgcagag gttgacgacg gtctcgatgc ggtcagtggg cacggtgctc tcctcggctg
     3301 gtctcaccgg ctggcggtcg gtgggcgaag ccctcagggt atcggcgggc gtaggacgcc
     3361 ctgcgccccc gccgtcagtc gaggtcgctg accacgaccg gagccgcggc ctccccgagg
     3421 ttgaccgcgc cggcgtcggc ggcgaggtcg ctgctggcac gcccgcccac gccggcctgc
     3481 tcgagcacct cgtaggcgct gggctcgtcg accgcgtcgt acatcaccgg gacggcccgc
     3541 agcttgacgt cgtaggacga gagcgcccgc cggtaggacc cgacgtcgcg aaacgtcagc
     3601 tccagcaccc acaggcccgc ctcgtcggtg ctgcgcccga cccggccgtc gacgaagccc
     3661 ggcgtcgcgg cgaaggcctc cagcgcgtcg cggatccgat cccggaacga cgcggcggtg
     3721 ccctcggaca cccggtagcg ggtcacgacc aacgtgtgca gcgccacgag cacctccgag
     3781 gatgggcgag gatggcaccc aggcattcag gctagagggc ggccgaacgg gccgagggaa
     3841 ggggcttcgt ggtggagcag cggcggcggg cggcgcgtcc gggcagcatg cgggccaggg
     3901 tcgagcaggc cagcgcaccg gcgctcgtca ggctgacccg gctgccccgg ctgctcgtgc
     3961 cggcgatctt cggggtgctg gtgatcgtcg cgctggccac cccggcgccg atcgccctgg
     4021 cggccaccgt ggtcgtggtc gcgttcgtgg gctggctgac ctacctgtcc tggccggcga
     4081 tcacgcccgg ggggcgggtg ttccggctcg tgacgctcgg gctgctcgtc ggctacctcg
     4141 tctggctgct gcaggggccg gccgcctgat ccgtcgtacc cctgccgggt ttgataaccg
     4201 ttttcattcc cggctaaggt gcgcgtcatg gccgtcctgc tcgccgtgac cacgctggtc
     4261 tccaccgccg ccggcgggtt cgtggcgctg cgggcgcgcg accggatgca cctcgtgctc
     4321 ggcttcgctg gcggggtgat gctcggcctg
//
LOCUS       DATIBB010000144        14818 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_87086, whole genome shotgun sequence.
ACCESSION   DATIBB010000144 DATIBB010000000
VERSION     DATIBB010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 14818)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14818)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14818
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87086"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..348
                     /locus_tag="VLC50_05550"
     CDS             <1..348
                     /locus_tag="VLC50_05550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSF26966.1"
                     /translation="ALATMLAGALLSVPDVLPLVLLGTATIVFGFFGAHSVASGWVNA
                     AASQARAQASALYLLFYHVGSSVAGFVGGLAFGVFGWGGEVATVIILLAAGALAAVRL
                     PPTSGPVEVPDAS"
     gene            338..1003
                     /locus_tag="VLC50_05555"
     CDS             338..1003
                     /locus_tag="VLC50_05555"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HSF26967.1"
                     /translation="MRPDEIAVALRRAVRERVISPGQALNQDELARRFGVSRIPLREA
                     LRTLVGEGLIIMKPGLGAVVTELQASEVNELYGLRLQLEPPLAPDIIDHVRRRDVDDL
                     EALVTAMQALGPEQSEEWSGLSYRFHRRLYERSERKHTMRLVVQVLNLVEPYNRVHAH
                     VLGSRARMTHEHADTVDALRSGDEARLAELITTGIVSARAELVAAMDQPMDVEGPAWY
                     DRL"
     gene            1164..2414
                     /locus_tag="VLC50_05560"
     CDS             1164..2414
                     /locus_tag="VLC50_05560"
                     /inference="COORDINATES: protein motif:HMM:NF024825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HSF26968.1"
                     /translation="MRSSKVVAVVSVAALTLLAAGCGSKGDAGATAANTGAQASAPSQ
                     ITIGASLSLTGKLAREGQLTKEGYELCEQKVNAKGGVTIGDSQAKLHIEYQDDTSKPD
                     TAAQLVDQFNDKGIKLILSSYGSANTEAQAAVIERNGQVMVDSAGADNKIFEKGYKRT
                     FAVLSPATEYAASIVKAIGELADPKPVSMAFLSADDGFSKTVTEGGIAEAKKEGFTVV
                     GPEFFPNGASDVSSSLTKVKGEKPDVIIGSVHLVEGVAIVKQAHELGVTPAGFGESVA
                     PPTPDFAKTLGAQAEGVLGSSQWTDATAGQDDYFGTAKDYSKDIQAAYGHAPDYHDAE
                     ASAACLALVLATQEAGSTDPDKVRDALAALDTDSFFGKIKFNETGQNTFKPMSVIQIQ
                     NGKVVTVWPTESAEAKMIWPGNAS"
     gene            2411..3295
                     /locus_tag="VLC50_05565"
     CDS             2411..3295
                     /locus_tag="VLC50_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020516480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HSF26969.1"
                     /translation="MSGFLQATIYGLLQGGLLALVAVGFSLVWGVMNVVNLAHGSFVI
                     VGAYVAWALNSWLGLDPFLGMFVAAAVLFVGGYAIQRGLINLVINGPIFITLLLTFGL
                     DLLIVQGMNLAFSANYRSIKTSYAGDSFAFGEVRVPLGRLFAFLVAIAVTLLLVVLVN
                     RTRTGMSIMATGMDRGAARLMGIRARHVYALTFGLAAALAGMAGAMVATVGTFNPAAA
                     GGFTLLSFVIAVLGGLGNMYGALAGGLMIGLVESWGGQFLSGTWVNALAFLVLVIVLI
                     VRPSGLVGRAFYSARVEV"
     gene            3295..4332
                     /locus_tag="VLC50_05570"
     CDS             3295..4332
                     /locus_tag="VLC50_05570"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HSF26970.1"
                     /translation="MATETTTRESTTTAPVIAPAWRRGWPWGLGLLGLLACFGLQTQL
                     YTGQERTVTTIFMFVALAQGWNLIGGFAGYASFGQVVFFGIGGYTTAVVMSHWQLSFW
                     LALPLSALVVTAFGVLIGLPLLRLKGHYFAIATLGVAEGMREVVTNLPELTGGGAGIT
                     VPTVGNQASTAYLGNDGFYTLFLLLAALAMLVTGLISRSKFGFALRAINQDEDAAASM
                     GINTTRTKVATFALSGFLTGLVGAAYAFQQVTIFPERLFDVDITVLMVVMVVIGGSGT
                     VLGPLLGAVALQFLSEWLRQHYTDFHTLILGAIVILAVILLPQGAVNYAKEAVRTREF
                     SLLANVRRYRL"
     gene            4329..5087
                     /locus_tag="VLC50_05575"
     CDS             4329..5087
                     /locus_tag="VLC50_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020516478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSF26971.1"
                     /translation="MSETPVLETQGLGRRFGGLQAVADVSFSVPSGQTLGVIGPNGAG
                     KSTFINLVTGHIKPTAGRVLIDGRDMTGARPWAIAKARVARTFQIVKPFRGMTVRENV
                     AVGVFYGPDGTGVMSQALARADEVLEEVGLAGSARRPPGELSVADARRLELAKALALR
                     PRLLLLDEVMAGLRLSEIEPSLELIARLKAQGMTIIAVEHVMKAILAISDKVLVLHQG
                     RVLTTGDPREVLSDPRVIEAYLGHRYAKRQEGKA"
     gene            5087..5779
                     /locus_tag="VLC50_05580"
     CDS             5087..5779
                     /locus_tag="VLC50_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013886661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSF26972.1"
                     /translation="MLEVSGLNAGYGRANVLWDVDLSVHDNEIVALVGPNGAGKTTLL
                     RALSGMAEIRSGSVSFRGRPTIGRSIEEIVDLGIAHVPEGRRLFSGLSVRDNLRLGGW
                     RTNNRDVDRVVELFPRLGERMDQIAGSMSGGEQQMCAIARGLMSQPELIMIDELSLGL
                     APVVVDEILDRLPDIAASGTSVLLVEQDVDAALSVAERAYVLETGHIVLSGKAGELLA
                     DPRVQESYLGVG"
     gene            complement(5810..6757)
                     /locus_tag="VLC50_05585"
     CDS             complement(5810..6757)
                     /locus_tag="VLC50_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020521889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043248 - proteasome assembly [Evidence
                     IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAC2 family protein"
                     /protein_id="HSF26973.1"
                     /translation="MLDPHSLIQLDPDLPELDGVVLLHALSGFVDAGAGVRLSREHLL
                     RSLEHRVLARFDVDQLLDYRARRPLMSFVEDHWESYPEPTLALHLVHDQAGTPFLLLT
                     GPEPDIQWERVVAAVRTLIDRFGVRLTIGLTAIPMAVPHTRPVGVTAHATRRELTAGR
                     ETWVSRVEVPASIGHLLEWRLGEAGRDAIGFAVHVPHYVAQIDYPGAAVALLEAAAKA
                     GGLMLPTGELVTAAETTLAQVEEQVRSSDEVAAVVHGLEAQYDAYVGARAGTNLLASD
                     GTPLPTADELGAELERFLADQARRHDEGEAGGSGEPGLS"
     gene            6879..7868
                     /locus_tag="VLC50_05590"
     CDS             6879..7868
                     /locus_tag="VLC50_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012895099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HSF26974.1"
                     /translation="MRILMIGGTRFVGRHIAGAAMARGHQVTLFHRGRTGAGLFPEVE
                     HRYGDRDTDLSALAEGQWDATVDVCAYLPGQVRALAQTLGERAGHYVLVSTVSVYDDP
                     PSPGATEAAPLVELDDPDVAVVTSETYGGLKVLCERAAVASFGAGTLAIRPTYVVGPD
                     DYTGRFPHWVERISRGGEVLLPGPADDPSQVIDVRDMGAWIVGLVEAGRAGALHAASP
                     RPPWSWADTMSAIADTVAPAGTTLTWVDAPFLRAQGVDDRSLPLWSGGDAGRFIFALD
                     PSAAYASGLSPRPLADTVRDTLAWLRSPEGAQRQHVGLSSEREDMLLRAWHER"
     gene            7902..9620
                     /locus_tag="VLC50_05595"
     CDS             7902..9620
                     /locus_tag="VLC50_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014742085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NERD domain-containing protein"
                     /protein_id="HSF26975.1"
                     /translation="MTVRCLPEQPRFESAAEAVVWQRLHDDLPDGSVLAANVAFSDRH
                     GDCEADLIVAVPGAGFAVVEVKGGHVTHDGTTWRQAGGGRSATPIDPVRQARRAKYAL
                     RGYLDRSPAWAGRCQVRLAHLVAFPYFRVPADFAPPDCPRWMVIDETDLADAVQRVLT
                     ATLLPDDERPLATPAEVDDLWAALTGRLLGQRELVSTVREHADAADLLTAEQAVVLDH
                     LRRVPRVEVRGGAGSGKTWLAVEKARRLARDGARVALMCYSRGLAAYLRRRVELMPPG
                     QRPAYVGTFHGLGERWGAPPGDRDDSDFWERRLPAAMVDLAARLPEVERYDAIVLDEA
                     QDFADAWWPAVLSGLRDPVHGGLYVFSDEGQRVFARYGRPPVELVPVELAENLRNTKQ
                     IAQTFGSLTTEQMRLRGGDGPPVRFVACATEEAVSAADDAVVGLLEDGWASEHVALLT
                     TGHRHPVQRERVEARGQDGYWASYWETDDVFYGHVLGFKGLERPAVVVAVNGFGNDHR
                     AREKLYVALSRARDLLVVCGDLELIRAAGGEGVVRRLSAAPASPRLLPPGVREASPGR
                     SAGCSG"
     gene            complement(9535..11007)
                     /locus_tag="VLC50_05600"
     CDS             complement(9535..11007)
                     /locus_tag="VLC50_05600"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HSF26976.1"
                     /translation="MTGSTGSTGSTTGGGSPLELDPGFQGIAIHPGEPGYDASRAVYN
                     GSIDRRPALIVRPSGTADVVDAVNAARRAGLPVAARGGGHSVAGNGVTEGGVQIDMSS
                     LKGTWVDPARHRARTNAGSLWGEFDRETQRYGLATPGGRVTTTGVAGFTTGGGYGWLS
                     PTFGLTCDNLISAEVVTADGRVVTASEDENPDLFWGLRGGSSNFGLATSFELAVHPVG
                     PTVLAGMLIHQLDTAVEVIMTYRDYVEQMPEELVTGVAIVQAPPAEFVPAEMVGVPVL
                     GILVLWIGDPDEGRTAVNGLRRIGPPLMDLVQPMPYTTFQTLLDPFNPRGQHNYHRGE
                     HLSGLPDAAIEAYVEAAPQTLSPLTQAVIFRHGGAVARVPEMAMAASHRDAAYMAHPI
                     TAWSDPADTAENMAWARQFSEAMRPFTTGGLYLNFETETTPEIVRRGFTAEKFDRLVE
                     LKDRWDPENLFRVNQNIPPSGRVTLPEPREAAAEVTQEQR"
     gene            11138..11689
                     /locus_tag="VLC50_05605"
     CDS             11138..11689
                     /locus_tag="VLC50_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006240297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HSF26977.1"
                     /translation="MTSTPASTVVSVIRLLDVVATAGSPSSYPGLVLPALAGAVNCDR
                     VTYDELAPSGAVRRVDHVVTAAGAGGDARHRLTLTLVVPGRGLVGITWRRARLAFSRA
                     EAELVDALRAPLATGVARAQGRLGQLTAREQQVLELVARGRTNVAIAHSLGISPRTVG
                     KHLEHCYRKLAVSSRAAAVASLR"
     gene            complement(11702..12892)
                     /gene="rocD"
                     /locus_tag="VLC50_05610"
     CDS             complement(11702..12892)
                     /gene="rocD"
                     /locus_tag="VLC50_05610"
                     /EC_number="2.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018092794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004587 - ornithine aminotransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HSF26978.1"
                     /translation="MTLAEERSTHNYHPLPVVISRAEGVWVEDIDGRRYLDALAGYSA
                     LNFGHRHPALVAAVREQLDRVTLTSRAFHHDLLGPFCAELADLAGMDLVLPMNTGAEA
                     VESAIKVARKWGYEVKGVAAEQASIVVAAGNFHGRTTTIVSFSTDPTARDGFGPFTPG
                     FRVVPFGDAAAVEAAIDGTTVAVLVEPIQGEAGVIIPPTGYLSALRELCTRRGVLLVL
                     DEIQSGLGRTGRTFAAEHEGVRPDLVALGKALGGGIIPVSAVVGDRAVLGVLKPGEHG
                     STFGGNPLAAAVGRAVIRLLETGELQRRAAELGAVMAERLNALVGHGVLAVRSRGLWA
                     GVDVDPALGTGRDVCEALMAHGVLAKDTHGSTVRLAPPLVIEPDELEWTMDRFAEAIA
                     SLRR"
     gene            13073..13210
                     /locus_tag="VLC50_05615"
     CDS             13073..13210
                     /locus_tag="VLC50_05615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF26979.1"
                     /translation="MLVLVIVFAVVAPALMLALATAAEDSLERSTSATARSRPSEPSD
                     G"
     gene            13298..13837
                     /locus_tag="VLC50_05620"
     CDS             13298..13837
                     /locus_tag="VLC50_05620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF26980.1"
                     /translation="MEWVLAVLAVLVLTGALTTALAVQLARALRRRARVLADRAGLRA
                     RSWGSGAPGELARARRELAESVEGLRRALAVSRETAAPIGDVPALLRRLERAAEVVDG
                     ELRVIEALRDPVRIAALLPGPQSRARALAESAERLGRGLVEAGAGAATDVAVLQADCA
                     LEAEALREVSRSARSPRPA"
     gene            complement(13803..14036)
                     /locus_tag="VLC50_05625"
     CDS             complement(13803..14036)
                     /locus_tag="VLC50_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013673003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl domain-containing protein"
                     /protein_id="HSF26981.1"
                     /translation="MTDVVFPPLSTDDPDGEGVVATWFVRDGEPVAVDQLLAEVALDK
                     VSVEVPAPVAGTVHLLVEEEEVVRQGEAIARID"
     gene            complement(14033..>14818)
                     /locus_tag="VLC50_05630"
     CDS             complement(14033..>14818)
                     /locus_tag="VLC50_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009081963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HSF26982.1"
                     /translation="MRPIVELMFADFFGVCFDQIYNHMAKIHFESGGNVKVPLVLTTA
                     VGGGYSDGAQHSQCLWGSFAHVPGLKVVVPSTPYDAKGLMTAAIHDDNPVVYMFHKGV
                     MGLPWMAKNPRATDVVPQESYEVPIGMARVAREGADVTVVTLSLSTHHVLDVAEKLAE
                     DDVLDAEVIDLRSLVPLDRETILRSVAKTGRLVVVDEDYLSFGLSGEIVATICDHDPG
                     MLRTAVARVAVPDVPIPYAHGLEQAVLPTRERIEAALHHVVRA"
BASE COUNT         2003 a         5212 c         5338 g         2265 t
ORIGIN      
        1 gccctggcca ccatgctcgc gggtgcgctg ctctcggtgc ctgacgtgct gccgctcgtg
       61 ctgctgggca ccgccacgat cgtgttcgga ttcttcgggg cgcactctgt ggccagcggc
      121 tgggtcaacg cggcggcctc acaggccagg gcccaggcct cggctctcta tctgctcttc
      181 taccacgtgg gttcgagcgt ggccggcttc gtcggtgggc tggcgttcgg cgtgttcggc
      241 tggggcgggg aagtggcgac ggtcattatc ttgctggccg ccggcgcgct ggccgccgtc
      301 cgtctgcccc ctacttctgg tcctgtggag gtacccgatg cgtcctgacg agatcgcggt
      361 cgcactgcga cgcgcggtgc gcgagcgcgt gatctcgccc gggcaggcgc tcaaccagga
      421 cgagctggcc cgtcgcttcg gtgtgagtcg gatccccctg cgtgaggcgc tgcgcaccct
      481 cgtcggcgag ggcctgatca tcatgaagcc gggcctgggc gcggtggtca ccgagctgca
      541 ggccagcgag gtcaacgagc tttacggtct gcgtcttcag ctggagccgc cgctggcccc
      601 cgacatcatc gaccacgtgc gacgccgcga cgtggacgac ctcgaggcgc tcgtcacggc
      661 gatgcaggcg ctgggcccgg agcagtcgga ggagtggtcg ggtctgagct atcggttcca
      721 ccggcggctc tacgagcgct ccgagcgcaa gcacacgatg cggctcgtgg tacaggtcct
      781 caacctcgtt gagccctaca accgcgtgca tgcccacgtg ctgggctcgc gggcgaggat
      841 gacccacgag catgccgaca ctgtggatgc cctgcgctca ggcgacgaag ctcggctggc
      901 cgagctcatc accacgggca tcgtcagtgc tcgggccgag ctggtggcgg cgatggacca
      961 gccgatggat gtcgaaggac ccgcctggta cgaccgcctc tagtgcggac gcggtcgggc
     1021 tgcgcagcac gaaaaggtgt agcaccgggc acgggtgggt aacgaccgaa atatccaata
     1081 tccagggtct tgtccgcatg accgtaatca ctgtatacgt tgcatagccc acccacccga
     1141 acccttcatc ggaggcagat gccgtgcgct catccaaggt cgttgctgtc gtttcagtcg
     1201 ccgcactcac ccttctggcc gcaggctgcg gctcgaaggg cgatgccggt gccaccgccg
     1261 ccaacactgg tgcgcaggcc agtgcgccta gccagatcac gatcggcgca tcgttgtcgc
     1321 tgaccggcaa gctggcccgg gaaggccagc tcaccaaaga gggctacgag ctgtgtgagc
     1381 agaaggtcaa cgccaagggc ggcgtaacca tcggagactc gcaggccaag ctgcacatcg
     1441 agtaccagga cgacacgtcc aagcccgaca ccgcggccca gctcgtcgac cagttcaacg
     1501 acaagggcat caagctgatc ctgagctcct acggctcggc caacaccgag gcgcaggccg
     1561 cggtcatcga gcgcaacggc caggtcatgg tggactccgc cggcgccgac aacaagatct
     1621 tcgagaaggg ctacaagcgc accttcgccg tcctctcgcc ggccaccgag tacgccgcct
     1681 cgatcgtcaa ggcgatcggc gagctggccg acccgaagcc ggtcagcatg gcgttcctgt
     1741 cggccgacga cggcttctcc aagaccgtga ccgagggcgg gatcgcggag gccaagaagg
     1801 agggcttcac cgtggtgggg ccggagttct tccccaacgg tgcctccgac gtgagctcgt
     1861 ccctcaccaa ggtcaagggc gagaagcccg acgtcatcat cggttcggtc cacctcgtcg
     1921 agggcgtggc catcgtcaag caggcccacg agctcggcgt cacgccggcc gggttcggtg
     1981 agagcgtcgc gccgcccacc cccgacttcg ccaagacgct cggcgcgcag gctgagggcg
     2041 tgctcggctc gagccagtgg accgacgcca ccgccgggca ggacgactac ttcggcaccg
     2101 cgaaggacta ctccaaggac atccaggccg cctacgggca cgcccccgac taccacgacg
     2161 ccgaggccag cgcagcttgc ttggccctcg tcctggcgac ccaggaggcc ggctcgaccg
     2221 accctgacaa ggtccgtgac gcgctcgcgg cgctggacac cgactcgttc ttcggcaaga
     2281 tcaagttcaa cgagacgggc cagaacacct tcaagcccat gtcggtcatc cagatccaga
     2341 acggcaaggt cgtcaccgtc tggccgaccg agtccgccga ggccaagatg atctggccgg
     2401 gcaacgcctc gtgagtggat ttctccaagc gaccatctac ggcctgctcc agggagggct
     2461 gctcgcgctc gtcgcggtcg gcttctccct ggtgtggggc gtgatgaacg tcgtcaatct
     2521 cgcccacggc tcgttcgtca tcgtcggggc ctacgtcgcg tgggcgctga actcctggct
     2581 cgggctcgac ccgttcctcg ggatgttcgt cgctgcggcg gtgctgttcg tcggcggtta
     2641 cgccatccaa cgagggctga tcaacctcgt catcaacggc ccgatcttca tcacgctgtt
     2701 gctgacgttc gggctcgacc tgttgatcgt gcagggcatg aacctcgcct tctccgccaa
     2761 ctaccgcagc atcaagacct cctacgccgg ggacagcttc gccttcggcg aggtgcgggt
     2821 gccgctcggc cgcctgttcg ccttcctggt cgccatcgcc gtgacgctgc tgctcgtcgt
     2881 gctggtcaac cgcactcgca ccgggatgtc gatcatggcc accgggatgg accgcggtgc
     2941 cgcccgcctg atggggatcc gggcacgaca cgtctatgcc ctgaccttcg ggctggcggc
     3001 ggcgctggcg ggcatggccg gggccatggt cgccaccgtg gggacgttca atcccgcggc
     3061 cgccggtggc ttcacgctgc tgtcattcgt gatcgccgtg ctcggggggc tcggcaacat
     3121 gtacggcgca ctcgccggcg gcctgatgat cggactggtc gagtcctggg gtggtcaatt
     3181 cttgtcgggc acctgggtca acgccttggc cttcctcgtg ctggtcatcg tgctgatcgt
     3241 gcggccgtcc gggctcgtcg gacgcgcctt ctactccgcg cgggtggagg tctgatggcc
     3301 accgagacca cgacccgaga gagcaccacc accgcgccgg taatcgcccc ggcctggcgc
     3361 cgggggtggc cgtggggtct gggactgctg gggctgctgg cctgcttcgg gctgcagacc
     3421 cagctctaca ccggccagga acgcaccgtc acgacgatct tcatgttcgt ggcgctggcg
     3481 cagggctgga acctcatcgg cggtttcgcc ggctacgcct ccttcggcca agtggtcttc
     3541 ttcggcatcg gcggctacac caccgccgtg gtgatgagcc actggcagct gagcttctgg
     3601 ctggccttac cgctgagcgc gctggtcgtc accgcgttcg gcgtactcat cgggctgccg
     3661 ctgctgcggc tgaaggggca ctacttcgcc attgccacgc tcggcgtcgc cgagggcatg
     3721 cgcgaggtgg tcaccaacct gcccgagctg acaggcggcg gtgccggcat cacggtgccc
     3781 accgtgggca accaggcgtc caccgcctac ctgggcaacg acgggttcta cacgctgttc
     3841 ctcctgctgg ccgccttggc gatgctggtg acgggcctca tctcgcgcag caagttcggc
     3901 ttcgcgttgc gggcgatcaa ccaggacgag gacgcggcag cgtcgatggg catcaacacg
     3961 acgcggacca aggtcgcgac ctttgccctc tccgggttcc tcaccggcct ggtcggagcg
     4021 gcctatgcgt tccagcaggt gaccatcttc ccggagcgcc tcttcgacgt cgacatcacc
     4081 gtgctcatgg tggtcatggt cgtcatcggc ggcagcggca ccgtcctggg gccgctgctc
     4141 ggcgccgtcg ccctgcagtt cctgtccgag tggttgcgtc agcactacac cgacttccac
     4201 acgctcatcc tgggtgccat cgtcatcctc gcggtgatcc ttctgccgca aggcgcggtg
     4261 aactacgcca aggaggcggt ccggacccgc gagttctccc ttttggccaa tgtccggagg
     4321 taccggttgt gagcgagacc cccgtgctcg agacgcaggg gctggggcga cggtttggcg
     4381 gcttgcaggc cgtggccgac gtctcgttca gcgtgccgtc ggggcagacg ctgggggtca
     4441 tcggccccaa cggcgccggc aagagcacct tcatcaatct ggtgaccggc cacatcaagc
     4501 cgacggccgg ccgtgtgctc atcgacggcc gggacatgac tggtgcccgg ccctgggcca
     4561 tcgccaaggc ccgcgtcgcg cgtaccttcc agatcgtcaa gcctttccgc ggcatgacgg
     4621 tgcgcgagaa cgtcgcggtg ggggtcttct acggccccga cggcaccggg gtgatgtcgc
     4681 aggcgctggc acgtgccgac gaggtgctcg aggaggtcgg gctcgccggc tcggcccggc
     4741 gacccccggg cgagctgtcc gtggccgatg cccgccggct cgagctggcc aaggccctgg
     4801 ccctgcgccc gcggctcctg ctgcttgacg aggtcatggc cgggttgcgg ctcagcgaga
     4861 tcgagccgag cctcgagctg atcgcgcggc tcaaggccca gggcatgacg atcatcgccg
     4921 tcgagcacgt gatgaaggcg atcctggcga tcagcgacaa ggtgctcgtg ctgcaccagg
     4981 gacgggtgct cacaaccggg gatccccgtg aggtgctctc ggacccgcgc gtcatcgagg
     5041 cctacctcgg gcaccgctac gccaagcgac aggaaggaaa ggcctgatgc tcgaggtgag
     5101 cgggctgaac gccggctacg gtcgcgccaa cgtgctgtgg gacgtcgatc tctcggtgca
     5161 cgacaacgag atcgtcgccc tggtcggccc caacggtgcc ggcaagacca cgctcctgcg
     5221 ggccctgtcc ggcatggccg agatccgctc gggctcggtg agcttccgcg gtcggccgac
     5281 catcggtcgc tctatcgagg agatcgtcga tctcggcatc gcccacgtgc cggaagggcg
     5341 gcggctgttc tccgggctca gcgtgcgcga caacctgcgg ctgggcggct ggcgcaccaa
     5401 caaccgagac gtcgaccgcg tcgtcgagct gttcccccgg ctgggggagc ggatggacca
     5461 gatcgccggc agcatgtccg gcggtgagca gcagatgtgt gcgatcgctc gtggcctgat
     5521 gagccagccg gagctcatca tgatcgacga gttgtcgctg ggtctggcgc cggtcgtggt
     5581 cgacgagatc ctcgaccggt tgcccgacat cgccgcgtcg ggcacgtcgg tactgctcgt
     5641 ggagcaggac gtcgacgccg ctctgtctgt ggccgaacgc gcctatgtcc tggagaccgg
     5701 ccacatcgtg ctgtccggca aggcggggga gctgctcgcc gatccgaggg ttcaggagag
     5761 ctacctcggc gtcggctgac gggcagcgtc ccggcgcgcc cggccgggct cagctcaggc
     5821 cgggctcgcc ggagccgcct gcctcgccct cgtcgtggcg gcgagcctgg tcggccagga
     5881 accgttcgag ctcggcgccc agctcgtcgg cggtcggcag cggcgtgcca tcggaggcca
     5941 gcaggttggt cccggcccgg gcaccgacgt aggcgtcgta ctgcgcctcg agcccatgca
     6001 ccaccgcggc cacctcgtcg gagctgcgga cctgctcctc gacctgcgcc agggtcgtct
     6061 cggccgccgt gaccagctcg ccggtgggca gcatcaggcc gccggccttg gccgcggcct
     6121 cgagcaacgc gacggccgcg cccgggtagt cgatctgggc gacgtagtgc ggcacgtgca
     6181 ccgcgaaccc gatcgcgtcg cggccggcct cgcccagtcg ccactcgagc aggtgcccga
     6241 tgctggccgg cacctcgacc cggctgaccc aggtctcgcg cccggccgtc agctcgcgcc
     6301 gggtggcgtg cgcggtgacc ccgaccggcc gggtgtgcgg cacggccatc gggatcgcgg
     6361 tgagcccgat cgtcagccgc accccgaacc ggtcgatcag ggtgcggacc gcggcgacca
     6421 cccgctccca ctggatgtcg ggctccggcc cggtcagcag caggaacggc gtgcccgcct
     6481 ggtcgtgcac caggtgcagc gcgagcgtcg gctcggggta gctctcccag tggtcctcga
     6541 cgaagctcat caggggccga cgggcccgat agtcgagcag ctggtcgacg tcgaagcggg
     6601 ccagcacgcg gtgctcgagg ctgcgcagca ggtgctctcg gctgagccga accccggcgc
     6661 cggcgtcgac gaacccgctc agggcgtgca ggaggacgac cccgtcgagc tctggcaggt
     6721 cggggtcgag ctggatcagg ctgtgcggat cgagcacggg ctcggggctc cctcgtcggt
     6781 gatgggcacg atggctgctg gacaccagca tgccctgtcc cgggcgtggc tcgcgtcggc
     6841 ggtcgccctc gcgcacgggg cgtggctagg gtgccgccgt gcgcatcctc atgatcggcg
     6901 ggacacgctt cgtcggccgg cacatcgctg gcgccgcgat ggcgcgcggg caccaggtca
     6961 cgctcttcca ccgcggccgc accggtgccg gactcttccc cgaggtcgag caccgctacg
     7021 gcgaccgcga cacggacctt tcggcgctgg ccgaggggca gtgggacgcc acggtcgacg
     7081 tctgcgccta cctgcccggc caggtccgcg cgctggcgca gacgttgggc gagcgggccg
     7141 ggcactacgt gctggtctcc acggtgagcg tgtacgacga cccgccgagc cccggggcca
     7201 cggaggctgc tccgctggtc gagctcgacg acccggacgt cgccgtggtg accagcgaga
     7261 cctacggcgg gctcaaggtg ctgtgcgagc gggcggccgt ggcctcgttc ggcgcaggga
     7321 cgctggcgat ccggccgacc tacgtcgtcg gccccgacga ctacaccggg cggttcccgc
     7381 actgggtcga gcgcatctct cgcggcggcg aggtgctgct gcccgggccg gcggacgacc
     7441 cgtcgcaggt catcgacgta cgcgacatgg gggcctggat cgtgggcctg gtcgaggcgg
     7501 gccgggccgg ggcgctgcac gccgcgagcc cccgcccgcc gtggtcgtgg gccgacacca
     7561 tgagcgcgat cgctgacacc gtcgccccgg ccggcacgac gctcacctgg gttgacgcac
     7621 ccttcctgcg ggcgcagggc gtggacgacc ggtcgctgcc gctgtggtcg ggtggcgacg
     7681 ccggccgatt catcttcgcg ttggacccgt cggcggccta cgccagcggc ctgtcaccgc
     7741 ggccgctggc cgacaccgtg cgcgacaccc ttgcgtggct gcgctcaccg gaaggcgccc
     7801 aacgccaaca tgttgggctc tcgtccgagc gcgaggacat gctgctgcgc gcgtggcacg
     7861 agcgctgagt gtcagcgcct ggcgaacgag gaggcacggg agtgacggtg cggtgcctgc
     7921 cggagcagcc gcgcttcgag tcggcggccg aggcggtggt ctggcaacgg ctgcatgacg
     7981 acctgcccga cgggtcagtt ctcgcggcca acgtggcgtt ctcagaccgg catggtgact
     8041 gcgaggccga cctcatcgtg gcggtgcccg gagcgggatt tgccgtggtg gaggtcaagg
     8101 gcgggcacgt gacccacgac gggaccacct ggcgccaggc cggtggtggg cgcagcgcta
     8161 cgccgatcga tccggtgcgc caggccagac gggcaaagta cgcgctgcgc ggctacctcg
     8221 accgcagccc cgcctgggcg ggccgctgcc aggtccggct cgcgcacctg gtcgcgttcc
     8281 cctacttccg ggtgcccgct gacttcgcgc cacccgactg cccgcgctgg atggtcatcg
     8341 acgagaccga tctcgcggac gccgtgcaac gagtcctgac cgccaccctc ctgcccgacg
     8401 acgagcgccc gctggccaca ccggccgagg tcgatgacct ctgggccgcc ctgaccggca
     8461 ggctgctggg ccaacgcgag ctggtcagca cggtgcgcga acatgccgat gccgcggacc
     8521 tgctcaccgc cgaacaggcc gtcgtcctcg accacctgcg ccgggtgccc cgggtggagg
     8581 tccgcggtgg cgccggctcg ggcaagacct ggctggccgt ggagaaggct cgtcgactag
     8641 cgcgtgacgg cgcccgggtc gcgctgatgt gctactcccg ggggctggcc gcctacctgc
     8701 ggcgtcgggt cgagctgatg ccgcctggcc agcgcccggc ctacgtcggc acgttccacg
     8761 gcctgggcga acggtggggg gcaccgcccg gggaccggga cgacagcgac ttctgggagc
     8821 gccgcctgcc ggcggcgatg gtggacctcg ctgcccggct gcccgaggtc gagcggtacg
     8881 acgcgattgt cctcgacgag gcccaggact tcgccgatgc atggtggccg gcggtgctgt
     8941 cgggcctgcg cgaccccgtc cacggcgggc tctacgtgtt ctccgacgaa gggcagcggg
     9001 tcttcgcccg ttatggccgg cctccggtcg agctcgtccc cgtcgagctg gccgagaacc
     9061 tgcgcaacac caagcagatc gcacagacct tcggcagcct gacgaccgag cagatgcggc
     9121 tgcgcggcgg ggacgggccg ccggtgcggt tcgtcgcgtg cgccaccgag gaggccgtct
     9181 cggccgcgga cgacgccgtg gtcgggctgc tggaggacgg ctgggcctcc gagcacgtcg
     9241 cgctgctgac caccgggcac cggcatccgg tgcagcgcga gcgcgtcgag gcgcgtggcc
     9301 aggacggcta ctgggcgtcc tactgggaga ccgatgacgt gttctacggc cacgtgctcg
     9361 gcttcaaggg cctggaacgg cccgccgtgg tggtcgccgt caacgggttc ggcaacgacc
     9421 accgggcccg ggaaaagctc tacgtcgcgc tgtctcgggc ccgcgacctc ctcgtggtgt
     9481 gcggtgacct cgagctcatc cgggcggccg gcggcgaggg cgtcgtacga cgtctcagcg
     9541 ctgctcctgc gtcacctcgg ctgctgcctc ccggggttcg ggaagcgtca cccggccgct
     9601 cggcgggatg ttctggttga cccggaacag gttctcggga tcccagcggt ccttgagctc
     9661 gacgagccgg tcgaacttct cggcggtgaa gccccgtcgc acgatctctg gggtggtctc
     9721 ggtctcgaag ttgaggtaga gcccgccggt ggtgaagggg cgcatcgcct cggagaactg
     9781 cctggcccaa gccatgttct ccgcggtgtc ggcgggatcg gaccacgccg tgatcgggtg
     9841 cgccatgtag gcggcgtcgc ggtggctggc cgccatggcc atctcgggca cccgggcgac
     9901 cgcgccgccg tggcggaaga tgaccgcctg ggtcagcggg gacagcgtct ggggggccgc
     9961 ctcgacgtag gcctcgatcg cggcgtcggg cagccccgac aggtgctcgc cgcggtggta
    10021 gttgtgctgg ccccgcgggt tgaacgggtc gagcagcgtc tggaacgtcg tgtagggcat
    10081 cggctggacg aggtccatca gcggcggccc gatccggcgc agcccgttga ccgcggtccg
    10141 gccctcgtcg gggtcaccga tccacaggac caagatgccc aggaccggta cgccgaccat
    10201 ctccgccggg acgaactcgg ccggaggggc ctgcacgatg gccacgcccg tgaccagctc
    10261 ctcgggcatc tgctcgacgt agtcgcggta ggtcatgatc acctcgactg cggtgtcgag
    10321 ctggtggatg agcatgccgg ccagcaccgt cgggccgacg gggtgcaccg cgagctcgaa
    10381 cgacgtggcc aggccgaagt tgctgctgcc accgcgcaga ccccagaaca ggtcggggtt
    10441 ctcgtcctcg ctggcggtga cgacccggcc gtcggcggtg accacctcgg cggagatgag
    10501 gttgtcgcag gtgagcccga acgtgggcga gagccagccg tagccgccgc cggtggtgaa
    10561 gcccgcgacg ccggtggtcg tgacccgccc gcccggggtg gccagcccat agcgctgggt
    10621 ctcccggtcg aactcgcccc acagcgagcc ggcgttggtg cgcgcccggt gccgggcggg
    10681 gtcgacccag gtgcccttca ggctcgacat gtcgatctgc accccgcctt cggtcacccc
    10741 gttgccggcg acgctgtgcc cgccgccgcg ggcggccacg ggcaggccgg cccggcgggc
    10801 ggcgttgacc gcgtcgacca cgtcggcggt gccgctgggc cgcacgatga gggccggcct
    10861 gcggtcgatc gacccgttgt agaccgcccg actcgcgtcg tagcccggct ccccggggtg
    10921 gatcgcgatg ccctggaagc ctgggtccag ctcgagcggt gagccgccgc cggtggtcga
    10981 accggtcgaa ccggtcgaac cggtcatggg gtcctccctc gtcgtcacgg acgtgcatgc
    11041 cttgacgcaa gccggtgcgc gcgggcaccg tcgataagca cctgtgcgta tgagcgcgac
    11101 tggtggctgg cgcggcacgc gggcgaggcg gagcacgatg acttccaccc cggcttccac
    11161 cgtggtctcg gtgatccggc tgcttgacgt cgtcgcgacg gcggggagcc cgtcgtcgta
    11221 ccccgggctg gtcctgcccg ccctggcggg cgcggtcaac tgcgaccgcg tgacctacga
    11281 cgagctggcg ccctcgggcg cggtgcgtcg ggtcgaccac gtcgtgacgg cggctggagc
    11341 cggtggggat gcccgccatc ggctcacgct cacgctggtc gtgcccggcc gggggctggt
    11401 cggcatcacc tggcggcggg cccggctggc cttcagccgg gccgaggccg agctcgtcga
    11461 tgcgctgcga gcaccgctgg ccaccggggt ggcgcgggcg cagggccggc tgggccagct
    11521 caccgcgcgc gagcagcagg tgctcgagct cgtggcgcgc ggccgtacca acgtggcgat
    11581 tgcgcactcg ctggggatca gcccacggac ggtcggcaag cacctcgagc actgctaccg
    11641 caagctcgcg gtcagcagcc gcgccgccgc ggtcgccagc ctgcgctgag cccgggcgag
    11701 gtcagcggcg cagcgacgcg atcgcctcgg cgaagcggtc catggtccac tcgagctcgt
    11761 cgggctcgat gaccagcggc ggggccagcc ggaccgtcga gccgtgcgtg tccttggcca
    11821 gcacgccgtg ggccatcaac gcctcgcaga cgtctcggcc ggtgcccagc gcggggtcga
    11881 cgtcgacacc ggcccacagc ccgcggctgc gcaccgccag cacgccgtgc ccgacgagag
    11941 cgttcagccg ctcggccatg acggccccga gctccgcggc gcgccgctgc agctcgccgg
    12001 tctcgagcag ccggatcacc gcccgcccga ccgcagcggc cagcgggttg ccgccgaacg
    12061 tgctgccgtg ctcgcccggc ttgagcaccc cgagcaccgc ccggtcaccg accacggcgc
    12121 tgaccgggat gatcccgccg ccgagcgcct tgcccagcgc gaccaggtcg gggcgcaccc
    12181 cctcgtgctc ggccgcgaac gtgcgcccgg tgcggcccag gcccgactgg atctcgtcga
    12241 ggacgagcag cacgcctcgt cgggtgcaca gctcgcgcag cgcgctcagg tagccggtcg
    12301 gcgggatgat cacgccggcc tcgccctgga tcggctcgac gagcacggcg acggtggtgc
    12361 cgtcgatcgc ggcctcgacc gccgcggcgt cgccgaaggg cacgacccgg aacccagggg
    12421 tgaacgggcc gaagccgtcg cgggcggtgg ggtcggtgga gaagctgacg atggtcgtcg
    12481 tacggccgtg gaagttgccg gccgcgacca cgatgctggc ctgctcggcg gccaccccct
    12541 tgacctcgta gccccacttg cgggccacct tgatcgcgct ctccacggcc tcggcgccgg
    12601 tgttcatcgg cagcacgaga tccatgccgg ccaggtcggc cagctcggcg cagaacgggc
    12661 cgagcaggtc gtggtggaag gcacggctcg tcagcgtgac ccggtcgagc tgctcgcgca
    12721 ccgcggcgac cagcgcgggg tggcggtggc cgaagttgag cgcggagtag ccggccagcg
    12781 cgtcgagata gcgccgcccg tcgatgtcct cgacccacac gccctcggct cggctgatca
    12841 ccaccggcag cgggtggtag ttgtgcgtcg agcgctcctc cgcgagggtg atcgcgtcga
    12901 gggttgcgct cgtcggctgt gtgcggttgt cggcgtccag cacgtgtctc gtcctcccgt
    12961 gagatgggtc cggcgacgat cggtctcggc ccatcgccac cgtacttggt caccggccgg
    13021 ccggcgggtc ggattgggtc atatggggtg aggggggagc acaatgggcg ccgtgctcgt
    13081 tctggtcatc gtcttcgccg tggtcgctcc cgctctgatg cttgccctgg ccacggcggc
    13141 cgaggactcc ctcgagcgct cgacgtctgc cacggcgcgc agcaggccga gcgaaccctc
    13201 ggacggctag ccgccaccag gcccaaccgg cggcgcgcga cctctgagcc ggcgcgctcg
    13261 ggtcgcgccg gtgggcgccc cgtcgtaccc tcgccgcatg gagtgggtgc tcgccgtgct
    13321 cgccgtcctc gtgctgaccg gtgcgctgac gacggcgctg gccgtgcagc tggcccgggc
    13381 gctgcgacgg cgggcccgcg tgctcgcgga ccgggccggg ctgcgcgccc ggtcatgggg
    13441 cagcggtgct cccggcgagc tggcccgcgc ccgtcgcgag ctggccgagt cggtcgaggg
    13501 gctgcgccgg gcgctcgcgg tgtcgcgcga gaccgcggcg ccgatcggcg acgtgcccgc
    13561 gctgctacgg cggctcgagc gggccgccga ggtggtcgac ggtgagctgc gggtgatcga
    13621 ggcgctgcgc gacccggtcc gcatcgctgc cctcctgccc ggaccgcagt cgcgggccag
    13681 ggctctcgcc gagtcggccg agcggctggg ccgcggcctg gtcgaggccg gggccggtgc
    13741 ggccaccgac gtcgcggtgc tgcaggccga ctgcgcgctg gaggccgagg ctttgcgcga
    13801 ggtcagtcga tccgcgcgat cgcctcgccc tgcctgacga cctcctcttc ctcgacgagc
    13861 aggtgcacgg tgccggccac cggggccggc acctccacgg agaccttgtc gagcgccacc
    13921 tcggcgagta gctggtcgac cgccaccggc tcgccgtcgc ggacgaacca ggtggccacc
    13981 acgccctcgc cgtcggggtc gtccgtcgac aggggcggga agacgacgtc ggtcacgccc
    14041 gcaccacgtg gtgcagggcc gcctcgatgc gttcccgggt cggcagcacc gcctgctcga
    14101 gcccgtgcgc gtaggggatg ggcacgtcgg gtacggcgac ccgggccacc gcggtccgca
    14161 gcatccccgg gtcgtggtcg cagatcgtcg cgacgatctc gccggacagc ccgaaggaca
    14221 ggtagtcctc gtcgaccacg acgagccggc cggtcttggc caccgagcgc aggatcgtct
    14281 cgcggtccag cggcaccagc gagcgcaggt cgatgacctc ggcgtcgagc acgtcgtcct
    14341 cggccagctt ctcggccacg tcgagcacgt ggtgcgtcga cagcgacagc gtcaccaccg
    14401 tgacatcggc gccctcacgg gcgacgcggg ccatcccgat cggcacctcg tagctctcct
    14461 ggggcaccac gtcggtcgcc cgagggttct tggccatcca cggcagaccc atgaccccct
    14521 tgtggaacat gtagacgacc gggttgtcgt cgtggatcgc cgcggtcatc aaccccttgg
    14581 cgtcataagg cgtgctcggt acgacgacct tcagcccggg cacgtgcgcg aaggagcccc
    14641 acaggcactg cgagtgctgg gccccgtcgg agtagccgcc gccgaccgcg gtggtcagca
    14701 cgagcggcac cttcacgttg ccgccggact cgaagtggat cttggccatg tggttgtaga
    14761 tctggtcgaa gcacaccccg aagaagtcgg cgaacatcag ctcgacgatc gggcgcat
//
LOCUS       DATIBB010000145         3134 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_1271469, whole genome shotgun sequence.
ACCESSION   DATIBB010000145 DATIBB010000000
VERSION     DATIBB010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3134
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1271469"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..927
                     /gene="fbaA"
                     /locus_tag="VLC50_05635"
     CDS             <1..927
                     /gene="fbaA"
                     /locus_tag="VLC50_05635"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013861556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HSF26983.1"
                     /translation="LNAALQGFAEAGSDGIVQISTGGAEYASGQAVKDMVAGAVALAE
                     YAHVVAANLPVTVALHTDHCPKDKLDGYMRPLIAVSQERVDAGREPLFQSHMWDGSAV
                     ELEENLSIAEELLEASARARLVMEMEIGVVGGEEDGVVGEMNEKLYSTPGDALRVAER
                     LGVGERGRYILAATFGNVHGVYKPGHVKLRPAVLREIQETVGAKVGRDKPFDLVFHGG
                     SGSALEEIRETLDYGVVKMNVDTDTQYAFTRPVAAHMFSNYDGVLKVDGDVGNKKAYD
                     PRSYGKAAEKSMAARVVRACEDLRSAGTSPTA"
     gene            988..1842
                     /locus_tag="VLC50_05640"
     CDS             988..1842
                     /locus_tag="VLC50_05640"
                     /inference="COORDINATES: protein motif:HMM:NF015587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjD/YihY/BrkB family envelope integrity
                     protein"
                     /protein_id="HSF26984.1"
                     /translation="MDQPSGRMARLWHRIEPAVRRAQAGLAGRVLGRLIEVRFLERSV
                     ILAGQAFSALLPLIIVVNTISPHPGGDTPAAALARRLGLDADEVSSLQAAAVPPASAR
                     ASIGVLGVVLAVLTATSFARALQRSYELAWRLPRPGLRAAWRPLALVIGLAVYVELLF
                     LFGRLVRGVPAGGLLEDLGTFAGAWVLWTAAGWMLLAAQVRPRLVAVGGLLTAIGFAL
                     LRRLSVFYLPDMVSSSHQQFGFLGVAFTLFSWLSACAFVIVVATVVGAVLAEDPGRLG
                     RFIRAPRR"
     gene            2002..2979
                     /locus_tag="VLC50_05645"
     CDS             2002..2979
                     /locus_tag="VLC50_05645"
                     /inference="COORDINATES: protein motif:HMM:NF024776.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HSF26985.1"
                     /translation="MSAGLTALGLDPAAERLYRELLRAPYGRRGRAPEPDDHNGHTGE
                     HSKAAAALVRLGLARRSEDGELVAITPGVALESVVDEELAKVEQRRRELEEVRVRIAE
                     LAAEHLVGQSEGWSPAPVLVVGTEVRAVIEDLARSVTGELLVLHEVPPQARGPWGVDV
                     AGVVRALTRRDQPVRCVYPVEALYEPEREAYVRFVIAQDCVVRLVERVPHRLLVFGHE
                     AAIVSSVDAARPVHHIVRAPELLRTVRSLFELYWESGVPFRRGDAPEEDARARLVELL
                     ATGMKDEAIARHLQLSLRTVRRRVAGLLDELGATTRFQAGMQAARRRLI"
BASE COUNT          390 a         1135 c         1145 g          464 t
ORIGIN      
        1 ctcaacgcgg ccctgcaggg cttcgccgag gccggctccg acggcatcgt gcagatctcc
       61 accggcggcg ccgagtacgc ctccgggcag gcggtcaagg acatggtggc cggcgcggtg
      121 gcgctggccg agtacgccca cgtggtggcg gccaacctgc cggtgaccgt ggcgctgcac
      181 accgaccact gccccaagga caagctcgac ggctacatgc gcccgctcat cgcggtctcg
      241 caggagcggg tcgacgccgg gcgggagccg ctgttccagt cgcacatgtg ggacggctcg
      301 gcggtcgagc tcgaggagaa cctctccatc gccgaggagc tgctcgaggc ctcggcccgg
      361 gcccggctgg tgatggagat ggagatcggc gtcgtcggcg gcgaggagga cggcgtcgtc
      421 ggcgagatga acgagaagct ctactcgacc cccggtgacg cgctgcgggt ggccgagcgg
      481 ctcggcgtgg gcgagcgcgg gcgctacatc ctggccgcga cgttcggcaa cgtgcacggg
      541 gtctacaagc ccggccacgt caagctgcgc ccggccgtgc tgcgcgagat ccaggagacc
      601 gtcggggcca aggtgggccg cgacaagccg ttcgacctgg tcttccacgg cgggtccggc
      661 tcggccctcg aggagatccg cgagaccctc gactacggcg tggtgaagat gaacgtcgac
      721 accgatacgc aatatgcgtt cacccgcccg gtcgctgcgc acatgttcag caactacgac
      781 ggcgtgctca aggtcgacgg cgatgtgggc aacaagaagg cctacgaccc gcgctcctac
      841 ggcaaggccg ccgagaagag catggccgcc cgggtggtcc gcgcctgcga ggacctccgc
      901 tccgccggca cctcccccac cgcctgagct cgcgccgcgg tctgctgacc gcggcgagcc
      961 cgatcggagc cgcgggggga gccgcacgtg gaccagccga gcggtcggat ggcccggctg
     1021 tggcaccgca tcgagcccgc cgtacgccgg gcgcaggccg ggctggccgg gcgggtcctc
     1081 ggccggctca tcgaggtcag gttcctcgag cgctcggtga tccttgccgg ccaggccttc
     1141 agcgcgctcc tgccgttgat catcgtggtc aacaccatca gcccgcaccc aggcggcgac
     1201 accccggccg cggccctggc tcgccggctc ggcctcgacg ccgacgaggt gtcgtcgctg
     1261 caggccgcgg ccgtcccgcc cgcctcggcg cgggcctcca tcggcgtgct cggcgtcgtc
     1321 ctggccgtcc tcaccgcgac cagcttcgcc cgggccctgc agcgcagcta tgagctggcc
     1381 tggcggctcc cccggcccgg tctgcgggcc gcctggcgtc cgctggccct ggtcatcggc
     1441 ctggccgtct acgtcgagct gctgttcctg ttcggccgcc tggtgcgcgg cgtcccggcc
     1501 ggcggcctgc tggaggacct ggggaccttc gccggcgcct gggtcctctg gaccgccgcg
     1561 ggctggatgc tgctcgccgc ccaggtccgc ccgcgcctcg tcgcggtcgg tgggctgctc
     1621 acggcgatcg gctttgcgct cttgcgtcgg ctgtccgtgt tctacctgcc cgacatggtc
     1681 tccagcagcc accagcagtt cgggttcctg ggggtggcgt tcacgctgtt cagctggctg
     1741 tcggcctgcg ccttcgtgat cgtggtggcc accgtcgtcg gcgccgtgct cgccgaggac
     1801 cccggtcggc tgggccggtt catccgcgcc ccgcggcgct gagctctcag cctgcccgcg
     1861 cgccatcgca gcgggggcca aaaagatgcg gcggcggaac tgtcccctgc cgcagttccg
     1921 ccgtcgcgtt agccatgagc atgcaccgtc cgagcccgat ctcgatggca tgaaggagcc
     1981 aatggcacta acctgccgca cgtgagtgca ggtcttaccg ctctggggtt ggatccggcg
     2041 gccgaacggc tctaccgcga gctgctccgc gccccgtatg ggcgccgggg tcgggcaccc
     2101 gagccggacg atcacaacgg ccacaccggc gagcactcga aggcggccgc ggcgctcgtc
     2161 cggctgggtc tcgcccggcg cagtgaggac ggcgagctcg tcgcgatcac ccccggagtc
     2221 gccctcgagt cggtcgtcga cgaggagctg gccaaggtcg agcagcgccg tcgtgagctc
     2281 gaagaggtcc gggtccgcat cgccgagctg gccgccgagc acctggtggg tcagtcggag
     2341 gggtggagcc cggcgccggt gctcgtggtc ggcaccgagg tccgggccgt catcgaggac
     2401 ctggcccgct cagtgaccgg cgagctgctg gtccttcacg aggtgccacc ccaggcgaga
     2461 ggaccctggg gcgtcgacgt ggccggcgtc gtgcgcgccc tgacccgccg cgaccagccg
     2521 gtgcgctgcg tctatcccgt cgaggcgctc tacgagcccg agcgggaggc ctacgtccgc
     2581 ttcgtcatcg cgcaggactg cgtcgtacgc ctggtcgagc gggtgcccca ccgcctgctg
     2641 gtgttcggcc atgaggcggc catcgtctcc tcggtggacg cggcccggcc ggtgcaccac
     2701 atcgtgcggg cgcccgagct gttgcgcacg gtgcgctcgc tgttcgagct ctactgggag
     2761 tcgggggtgc cgttccggcg cggcgacgcg cccgaggagg acgcccgggc ccgcctcgtc
     2821 gagctgttgg ccaccgggat gaaggacgag gcgatcgccc ggcacctgca gctgtcgctg
     2881 cggaccgtac ggcgacgggt ggccgggctg ctcgacgagc tgggcgccac cacccggttc
     2941 caggccggca tgcaggccgc ccggcgccgc cttatctagg ccgcgcgccg cgggatagcc
     3001 tctgtgcgca acggccgctg ctggggacct cgcccgtcct gtgccggccc cttcgttcga
     3061 aaggtcaccc agatgcccgc agtcgtgctc atcggcgccc agtggggcga tgagggcaag
     3121 ggcaaggcga ccga
//
LOCUS       DATIBB010000146        12163 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_746898, whole genome shotgun sequence.
ACCESSION   DATIBB010000146 DATIBB010000000
VERSION     DATIBB010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 12163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12163
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_746898"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2575)
                     /gene="aceE"
                     /locus_tag="VLC50_05650"
     CDS             complement(<1..2575)
                     /gene="aceE"
                     /locus_tag="VLC50_05650"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020521423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HSF26986.1"
                     /translation="MASERQQIISNGLPSQLPDIDPDETQEWLDSLDQAVAQRGRTRA
                     RYLMLSLLRRARENNVGVPSLRSSDYINTIPPEAEPWFPGDEYVERRIRAYIRWNAAM
                     MVHRAQRPGVAVGGHISTYASSASLYEVGFNHFFRGKDHPGGGDQVFFQGHASPGMYA
                     RAYLEGRLTEDQLDGFRQEHSHPSGGLPSYPHPRLMPDFWEFPTVSMGLGPMSAIYQA
                     RFNKYLHNRGIKDTSQQHVWGFLGDGEMDEVESLGAIGVAAREELDNLTFVINCNLQR
                     LDGPVRGNGKIIQELESFFRGAGWNVIKVIWGREWDPLLASDRDGALVNLMNVTPDGD
                     YQTYKAESGAFVRENFFGRDERTLRMVRNLSDDQIWNLKRGGHDYRKLYAAYDAAMKH
                     TGQPTVILAKTIKGWTLGSHFEGRNATHQMKKLTLDDLKAFRDRLFIPISDEALEANP
                     KMPPYYNPGQNSDEMRYMRERRQRLGGSLPRRRTDWTRTIKLPDDTPYEVVRRGSGKQ
                     PIATTMAFVRLIKEMMKDKEFGKRFVPVIPDEARTFGMDSLFPTAKIYSPHGQTYMSV
                     DRDLMLAYREATDGQILHEGINEAGSVASWTAAATSYATHGEPMIPIYIFYSMFGFQR
                     AGDGLWAAGDQMARGFLLGATAGRTTLTGEGLQHADGHSHLLASAYPAVVSYDPAYAY
                     EIGHIVKDGLRRMYGDAPENIYYYLTVYNEPMPQPAEPEGLDVEGLLKGAYLLSRSPY
                     ADEDRPRAQLLASGVGVPWALEAQSLLINDWGVAADVWSVTSWTELRRDGLAADEWNL
                     MHPDEAPRVPYLTRVFENQPGPRVAVSDFMRAVQDQIAPWMPGDFVSLGTDGFGFSDT
                     RA"
     gene            2884..3309
                     /locus_tag="VLC50_05655"
     CDS             2884..3309
                     /locus_tag="VLC50_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012852007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3052 domain-containing protein"
                     /protein_id="HSF26987.1"
                     /translation="MSATADHAEGHAPTTRLGIQQGHVVQEIGYDDDCDEALRTAIEA
                     ASGAAMVGEDFDDVVDCVVLWWREGDGDLVDALVDSLVSLADGGVIWLLTPKVGREGH
                     VEPSDIGDAAPTAGLSATSTVAAAPDWSGTRLVAPRVRR"
     gene            3334..3792
                     /locus_tag="VLC50_05660"
     CDS             3334..3792
                     /locus_tag="VLC50_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018103345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HSF26988.1"
                     /translation="MTIEIGTAAPDFALPDQHGRTVRLSDFHGHKNVVVVFYPFSFTG
                     TCTGELCAIRDEIGDFANDDVQVLAISCDSKHTQRVFAEQEGYEFPLLSDFWPHGEVA
                     QAYGVFDEHAGRATRGTFLVDREGVVRWSVVNDPSEARRTDDYRKALAQL"
     gene            3832..3903
                     /locus_tag="VLC50_05665"
     tRNA            3832..3903
                     /locus_tag="VLC50_05665"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3864..3866,aa:Val,seq:tac)
     gene            4000..5172
                     /locus_tag="VLC50_05670"
     CDS             4000..5172
                     /locus_tag="VLC50_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013117371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HSF26989.1"
                     /translation="MPQTPRLADVIATLDALYDPGWALSWDAVGLVCGDPAAPVRKVL
                     FAVDPVDVVVDEALAWGADLVVTHHPLFLRAVHGVAATTPKGRVVHRLITAGVALHVA
                     HTNADVAEHGVSEALAARLGLTGTRPLVASPAEPLDKIVTFVPDEAADRVVDALAAAG
                     AGAIGAYTRCAWTTTGTGTFTPQAGAQPAIGVVGQVELVPETRVEMVMARHRRQAVVA
                     ALRAAHPYEQPAFDVLELAGWPGPRGIGRVGELATPTRFADLLDHVAARLPLGPAGLR
                     AAGDPDLLVRRVAVCGGAGDDLFDDVRRSGADVYVTADLRHHPAAEALDRGGPALVDA
                     GHWSTEWPWLAEAATRMAAGLAERGATVETRVSTTCTDPWTRHVPPALAPDPGSSS"
     gene            5169..5912
                     /locus_tag="VLC50_05675"
     CDS             5169..5912
                     /locus_tag="VLC50_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018839080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HSF26990.1"
                     /translation="MNAAPHDQQRLLDLQAMDTRLDQLAHRRKGLPEHAELAAHEARL
                     AELRDLTVAATTEVSDIEREQAKAEADVDQVRQRAERDQSRLDSGQVTSAKELESLQH
                     EIASLLRRQGDLEDVQLEVMERLESAQARVGELAAETERVCAARDETVARRDASYAEI
                     DAEAATTGQLRSVLAGQIDAALVTLYEKVRESSGGVGAAALHQRRCEGCRLELNNVDL
                     GRFRAAPPDEVLRCEECRRILVRTDESGL"
     gene            5909..6832
                     /locus_tag="VLC50_05680"
     CDS             5909..6832
                     /locus_tag="VLC50_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018556867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase-like protein"
                     /protein_id="HSF26991.1"
                     /translation="MSRRLIIEADGGSRGNPGPAAYGALVRDADTGEVLAERAEHIGR
                     ATNNVAEYRGLVAGLEAVREIDPTAQLEVRMDSRLVIEQMSGNWKIKHPDMVPLARRA
                     RGLLPTGKVTWTWVPRERNKNADRLLNIELDRATGRGGLGSMASPRLDRPRLDPDAPP
                     ATRPATPATGAAGSPAALVAAFHEAFDLSRQAHPDASAVPGDVARLRQDLLEEEVAEL
                     GRAAADVDVVAVARELADVVYVAYGTALTYGIDLDAVLAEVHRANMSKLGPDGRPRRR
                     ADGKVLKGDGFAEPDVAAVLGLAPPQPPAGA"
     gene            complement(6811..7371)
                     /locus_tag="VLC50_05685"
     CDS             complement(6811..7371)
                     /locus_tag="VLC50_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018382066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HSF26992.1"
                     /translation="MRTITAGLFISLDGVVEAPDQWHFPYFNDEMGAAVDASIGAADT
                     LLFGRKTYDSFAGAWPEREEAGGEDADFAKKLGDMRKIVVSHQQLDLGWRNSEQLQGD
                     LVDAVTALKNEPGGDIALSGSVSVVRQLLAAGLLDELALLVHPIAVRKGMRLFDEGDT
                     TIALRLVSSETFQTGVLNLVYAPAGG"
     gene            complement(7460..8242)
                     /gene="yaaA"
                     /locus_tag="VLC50_05690"
     CDS             complement(7460..8242)
                     /gene="yaaA"
                     /locus_tag="VLC50_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018093303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HSF26993.1"
                     /translation="MLVLLPPSEGKASASRGRTLDLCGLSFPELTATRHAVLDSLVHV
                     CRSQPADAVRRLGLSPGLVGEVARNADLETAATLPVARLYTGVLYDALDLASLDAPAR
                     RRATRNLVVVSALFGALRLGDRVPRYRLAMNADLPGIGPLAAAWREPLSRVLPAGIGR
                     GLLVDLRSSTYAAAWVPPPALAERTVAVRVLREHAGRRTVVSHMAKHTRGELARELVQ
                     APTAPRTPAELAELAGARWTAELTAPARAGRVWALDLVINEG"
     gene            8293..9732
                     /locus_tag="VLC50_05695"
     CDS             8293..9732
                     /locus_tag="VLC50_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015608207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HSF26994.1"
                     /translation="MPRRLLRLRADGGLLRARFAAIRDELGVPDGFSSPVLAEAEAAT
                     SAAAGVSGVPGGPAAPEDRTDLPLLTIDPPGSMDLDQALHLQRHGRGYRVHYAIADVA
                     TFVRPGGAIDAEAHRRAETIYAPDERAPLHPPALSEGAASLLPGQVRPAVLWTIDLDA
                     DGELIGADLRRALVRSRQRWDYEAVQRGVDAGDERWALLGEIGRLRLAREVERGGVDL
                     PIPEQEVVPSGAGFALAYRTVVPAERWNAQISLLTGIAAARLMVAGRVGVLRTLPAAP
                     NGRTELLRRTASALGIDWPAGDTFAELVHSLDPARPEVAAFVQEATSLLRGAGYVSFD
                     GELPPEHEHAAVASVYAHVTAPLRRLVDRYATECCLALVEGRDVPEWVRARLAELPKT
                     MAVGDQLAARLERACLDAVEAAVLAGAVGRVFPAVVVEVREGGGVVQLREPAVRARCD
                     GEALPLGELVDARLVEADVQTGTVRFALA"
     gene            9771..10152
                     /gene="rnpB"
                     /locus_tag="VLC50_05700"
     ncRNA           9771..10152
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VLC50_05700"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            10447..10650
                     /locus_tag="VLC50_05705"
     CDS             10447..10650
                     /locus_tag="VLC50_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012785683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HSF26995.1"
                     /translation="MAQGTVKWFNDEKGYGFISVDDGSKDVFVHFSAISGEGFRSLQE
                     AQRVEFDITQGPKGPQAENVRPL"
     gene            complement(10753..11601)
                     /gene="map"
                     /locus_tag="VLC50_05710"
     CDS             complement(10753..11601)
                     /gene="map"
                     /locus_tag="VLC50_05710"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020573257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HSF26996.1"
                     /translation="MALLIPGTISPTRPVPQHIRRPSYVGHPAPAPYDGPEVKDAETI
                     ELMRVAGQIAAAALVEVSTHVAPGVSTDELDRVGHEFLCDHGAYPSTLGYRGFPKSLC
                     TSVNEVICHGIPDSTELRDGDIVNVDITAFIDGVHGDTDATYLVGEVDEASRLLVERT
                     HEAMMRGIKAVAPGRQVNVIGRVIEAYARRFGYGVVRDFTGHGIGSAFHSGLIIPHYD
                     DPAYDTVIEAGMTFTIEPMLTLGSADWEMWDDGWTVVTRDRRRTAQFEHTLLVTETGA
                     EILTLA"
     gene            11653..11832
                     /locus_tag="VLC50_05715"
     CDS             11653..11832
                     /locus_tag="VLC50_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020546284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF26997.1"
                     /translation="MSDRWYYCLKHDTVEPEAGCANKDRLGPYATREEAADALALAAR
                     RNEAWAAADDEDDDD"
     gene            complement(11980..>12163)
                     /locus_tag="VLC50_05720"
     CDS             complement(11980..>12163)
                     /locus_tag="VLC50_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017591004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF26998.1"
                     /translation="EPAPAPDDGGSLVSMATVQHLVSTAAGEPDRAGRRGLRERLSRF
                     LDAVSGPAPEGSRPRW"
BASE COUNT         1663 a         4285 c         4518 g         1697 t
ORIGIN      
        1 ccgcgcgggt gtcggagaac ccgaacccgt cggtgcccag cgagacgaag tcgccgggca
       61 tccacggggc gatctggtcc tgcacggcgc gcatgaagtc gctgaccgcg acgcgcggac
      121 cgggctggtt ctcgaacacc cgggtcaggt agggcacgcg gggcgcctcg tcggggtgca
      181 tgaggttcca ctcgtcggcg gccagcccat cgcggcgcag ctcggtccac gaggtcaccg
      241 accacacgtc ggcggccacg ccccagtcgt tgatcagcaa cgactgggcc tccagcgccc
      301 acggcacccc gacgcccgag gcgagcagct gggcgcgcgg gcggtcctcg tcggcgtagg
      361 gcgagcggct gagcaggtag gcacccttga gcagaccctc gacgtcgagg ccctcgggct
      421 cggccggctg cggcattggc tcgttgtaga cggtgaggta gtagtagatg ttctccgggg
      481 cgtcgccgta catgcggcgt agcccgtcct tgacgatgtg cccgatctcg taggcgtagg
      541 ccgggtcgta ggagaccacc gcggggtagg ccgaggccag caggtgcgag tgcccgtcgg
      601 cgtgctgcag gccctcgccg gtcagcgtgg tgcgaccggc ggtggcgccg agcaggaagc
      661 cacgggccat ctggtcgccg gcggcccaca ggccgtcgcc ggcccgctgg aacccgaaca
      721 tcgagtagaa gatgtagatc gggatcatcg gctcgccgtg ggtggcgtag gacgtggccg
      781 ccgcggtcca ggaggcgacc gagccggcct cgttgatgcc ctcgtgcagg atctgcccgt
      841 cggtggcctc gcggtaggcg agcatgaggt cgcggtcgac ggacatgtag gtctgcccgt
      901 gcggcgagta gatcttggcc gtcgggaaca gcgagtccat gccgaaggtg cgggcctcgt
      961 cggggatgac cggcacgaac cgcttgccga actccttgtc cttcatcatc tccttgatga
     1021 gacggacgaa ggccatcgtc gtggcgatcg gctgcttgcc cgagccgcgc cgtacgacct
     1081 cgtagggcgt gtcgtcgggc agcttgatgg tgcgggtcca gtcggtacga cgacgcggca
     1141 gcgagccgcc caggcgctga cgacgctcgc gcatgtagcg catctcgtcg gagttctgcc
     1201 cggggttgta gtagggcggc atcttggggt tggcctcgag ggcctcgtcg gagatgggga
     1261 tgaacagccg gtcgcggaac gccttgaggt cgtcgagggt gagcttcttc atctggtggg
     1321 tcgcgttgcg gccctcgaag tggctgccca gggtccagcc cttgatggtc ttggcgagga
     1381 tcaccgtcgg ctgcccggtg tgcttcatcg ccgcgtcgta ggcggcatag agcttgcggt
     1441 agtcgtgccc gccgcgcttg aggttccaga tctggtcgtc ggagaggttg cggaccatcc
     1501 gcagcgtgcg ctcgtcgcgg ccgaagaagt tctcgcgcac gaacgcgccg gactcggcct
     1561 tgtaggtctg gtagtcaccg tccggggtga cgttcatgag gttgaccagc gcaccgtcgc
     1621 ggtcggaggc gagcagcggg tcccactcgc ggccccagat caccttgatg acgttccagc
     1681 cggccccgcg gaagaacgac tcgagctcct ggatgatctt gccgttgccg cggaccgggc
     1741 cgtcgaggcg ttgcaggttg cagttgatga cgaacgtgag gttgtcgagc tcctcgcgcg
     1801 cggccacgcc gatggcgccc agcgactcga cctcgtccat ctcaccgtca ccgaggaagc
     1861 cccacacgtg ctgctgggag gtgtccttga tcccacggtt gtgcaggtac ttgttgaacc
     1921 gagcctggta gatcgcgctc atcgggccga ggcccatcga cacggtgggg aactcccaga
     1981 agtcgggcat gagccgcggg tgcgggtagg acggcagccc gccgctgggg tgggagtgct
     2041 cctggcggaa cccgtcgagc tggtcctcgg tgagccggcc ctcgaggtag gcgcgggcgt
     2101 acatgccggg ggaggcgtgc ccctggaaga acacctggtc gccgccgccg gggtggtcct
     2161 tgccgcggaa gaagtggttg aagccgacct cgtagaggct ggcgctggag gcgtaggtcg
     2221 agatgtgccc acccacggcc acgccgggac gctgggcgcg gtgcaccatc atcgcggcgt
     2281 tccagcggat gtaggcccgg atccgccgct cgacgtactc gtcgccgggg aaccagggct
     2341 cggcctcggg cgggatcgtg ttgatgtagt cgctgctgcg caggctgggc acgcccacgt
     2401 tgttctcgcg ggcgcggcgc agcaggctga gcatcaggta acgcgcccgg gtgcggcccc
     2461 gctgggccac cgcctggtcg agcgagtcaa gccactcctg agtctcgtcc gggtcgatgt
     2521 ccggcagctg gctcgggagg ccgttgctga tgatctgctg tcgctcgctg gccacgtgag
     2581 tccttccacg ccggctgtgc cgacgcttta ctcgtctccc gccccttgtg agggctcgaa
     2641 cacccacatc gtgctacgcg gcccggtgag ccgtcataca cgtctcacgg taacgctcgc
     2701 cgcgctgtgg ccgggcgccg ggaccgcccg aggcaccccg ccggtggccg ggtcgagacc
     2761 ggccaatccg acatcgacgc gccgaaacgc ttgcatgagg gcctccgctc cgctggacta
     2821 cccgcgagca ggccggccgc gacggccgac ggcatccatg cccgcgaccc aggaggagac
     2881 accgtgagcg cgaccgcgga ccacgcggag ggtcacgcac cgacgacccg gctgggcatc
     2941 cagcaggggc acgtggtcca ggagatcggc tacgacgacg actgcgacga ggcgctgcgt
     3001 acggcgatcg aggccgcgtc gggtgccgcg atggtcggcg aggacttcga cgacgtcgtc
     3061 gactgcgtcg tgctgtggtg gcgtgagggc gacggcgacc tcgtcgacgc gttggtcgac
     3121 tcgctagtct cgctcgcgga cggcggggtg atctggctgc tcacccccaa ggtgggtcgt
     3181 gaggggcacg tcgagcccag cgacatcggc gatgccgcgc cgacggcggg gctgtcggcg
     3241 acctcgaccg tggctgcggc acccgactgg tcgggcaccc ggctggttgc gccccgcgtc
     3301 cgccgctgag acccgccgcg caccaggaga gacatgacca tcgagatcgg caccgccgcg
     3361 cccgacttcg cgctgcccga ccagcacgga cgcaccgtgc gcctgtccga cttccacggc
     3421 cacaagaacg tcgtcgtggt gttctacccg ttctcgttca ccggcacctg caccggcgag
     3481 ctgtgcgcga tccgcgacga gatcggcgac ttcgccaacg acgacgtcca ggtgctggcc
     3541 atctcctgcg actccaagca cacccagcgg gtcttcgccg agcaggaggg ctacgagttc
     3601 ccgctgctgt ccgacttctg gccgcacggc gaggtcgccc aggcctacgg cgtgttcgac
     3661 gagcacgcgg gtcgcgcgac ccgggggacc ttcctcgtcg accgcgaggg cgtggtgcgc
     3721 tggagcgtgg tcaacgaccc cagcgaggcg cggcgcaccg atgactaccg caaggccctc
     3781 gcgcagctct gagtcggtcc gggtgcggcc ggtaggctgc cctgctggtc agggcgtata
     3841 gctcagcggt agagctccgg tcttacacac cggcggtcgg cggttcgaat ccgtctgcgc
     3901 ccaccccgtc cccacgtcgg tgatcagcca gcttcggtac ggcgtccgcg cccgatgcgc
     3961 cggggcgatc gggacaaccg atccggatag cctgcccccg tgccgcagac cccccggctc
     4021 gccgacgtga tcgccacgct cgacgcgctc tacgacccgg gctgggccct gtcgtgggac
     4081 gcggtcggcc tggtctgcgg cgacccggcg gcgccggtcc gcaaggtgct gttcgccgtc
     4141 gacccggtcg acgtcgtggt cgacgaggcg ttggcctggg gcgccgacct ggtggtgacc
     4201 caccacccgc tcttcctgcg cgccgtgcac ggggtcgcgg cgaccacccc caagggcagg
     4261 gtcgtgcacc ggctgatcac cgccggggtg gcgctgcacg tggcgcacac caatgccgac
     4321 gtcgccgagc acggcgtgtc cgaggcgctg gccgcccggc tcgggctgac cggcacccgt
     4381 ccgttggtcg cctcgccggc cgagccgctc gacaagatcg tcacgttcgt ccccgacgag
     4441 gccgccgacc gcgtcgtcga tgcgctggcc gccgccgggg cgggcgcgat cggcgcctac
     4501 acccggtgcg cctggacgac gaccggcacc ggcacgttca ccccccaggc cggggcgcag
     4561 cccgcgatcg gcgtggtcgg gcaggtcgag ctggtgcccg agacccgggt cgagatggtg
     4621 atggcccgcc accggcggca ggccgtcgtc gccgcgctgc gcgccgccca cccctatgag
     4681 cagccggcgt tcgatgtgct cgagctggcc ggctggccgg gcccgcgagg catcgggcgg
     4741 gtgggcgagc tggccacccc gacgcgtttc gccgacctgc tcgaccatgt ggccgcccgg
     4801 ctgccgctcg gcccggccgg gctgcgcgcg gccggcgacc ccgacctgct ggtgcgacgg
     4861 gtggcggtct gcggcggtgc cggcgacgac ctgttcgacg acgtacgacg gagcggcgcc
     4921 gacgtctacg tgaccgccga cctgcgccac cacccggccg ccgaggcgct cgaccgaggt
     4981 ggcccggccc tggtggacgc cgggcactgg tcgaccgagt ggccgtggct ggccgaggcc
     5041 gccacgcgga tggctgcggg gctggccgag cgcggcgcta cggtggagac ccgcgtgtcg
     5101 accacgtgca ccgatccgtg gacccggcat gtcccgcccg ccctcgcgcc tgatcccggg
     5161 agctcctcct gaacgccgca cctcacgacc agcagcgcct gctcgacctg caggcgatgg
     5221 acacccggct cgaccagctg gcccaccggc gcaaggggct tcccgagcac gccgagctcg
     5281 ccgcccacga ggcccggctc gccgagctgc gcgacctgac ggtcgccgcc accaccgagg
     5341 tctccgacat cgagcgcgag caggccaagg ccgaggccga cgtcgaccag gtgcgccagc
     5401 gggccgagcg cgaccaatcc cggctcgact cggggcaggt gacctcggcc aaggagctcg
     5461 agagcctgca gcacgagatc gcgtcgttgc tgcgccggca gggcgacctc gaagacgtcc
     5521 agctcgaggt gatggaacgc ctcgagagcg cccaggcccg ggtcggtgag ctggccgccg
     5581 agaccgagcg ggtctgcgcg gcccgcgacg agaccgtggc ccgccgtgat gcgtcgtacg
     5641 ccgagatcga tgccgaggcc gcgaccaccg gccagctgcg ctcggtgctc gcagggcaga
     5701 tcgacgcggc gctggtgacg ctctatgaga aggtccgcga gtcctccggc ggggtggggg
     5761 ccgccgcgct gcaccagcgg cggtgcgagg gctgccgtct cgagctcaac aacgtcgacc
     5821 tgggtcggtt ccgggccgcg ccgcccgacg aggtgctgcg ctgcgaggag tgccgacgca
     5881 tcctcgtgcg gaccgacgag tccgggctgt gagccggcgg ctgatcatcg aggccgacgg
     5941 cggctcgcgc ggcaaccccg gcccggccgc ctacggcgcg ttggtccgcg acgctgacac
     6001 cggcgaggtg ctggccgagc gggccgagca catcggccgg gccaccaaca acgtcgccga
     6061 gtaccgcggg ctggtcgccg ggctcgaggc ggtgcgcgag atcgacccga ccgcccagct
     6121 cgaggtgcgg atggactcgc ggctggtcat cgagcagatg tcgggaaact ggaagatcaa
     6181 gcaccccgac atggtgccgc tggcgcgccg ggcccgtggc ctgctgccga ccgggaaggt
     6241 cacgtggacc tgggtgccgc gggagcgcaa caagaacgcc gaccggctgc tcaacatcga
     6301 gctcgaccgg gccaccggcc gcgggggact gggctcgatg gcatcgcccc ggctcgaccg
     6361 gccgcggctc gaccccgacg cgccgcccgc cacgcggccc gccacgccgg ccacgggcgc
     6421 ggccggctca ccggccgcgc tggtcgcggc attccacgag gccttcgacc tgtcccggca
     6481 ggctcacccg gacgcgtccg cggtgcccgg cgacgtggcc cggctgcgcc aggacctgct
     6541 cgaggaggag gtcgccgagc tgggccgggc cgccgccgac gtcgacgtcg tcgcggtggc
     6601 ccgcgagctg gccgacgtcg tctacgtcgc ctacggcacc gcgctgacct atggcatcga
     6661 cctcgacgcg gtcctcgccg aggtgcaccg ggccaacatg agcaagctcg gccccgacgg
     6721 gcgcccgcgc cgacgggccg acggcaaggt cctcaagggc gacgggttcg ccgagcccga
     6781 cgtcgccgcg gtcctcggcc tggccccgcc tcagccgcct gccggcgcat agaccaggtt
     6841 gagcacgccg gtctggaacg tctccgagga gaccagccgc agcgcgatcg tcgtgtcgcc
     6901 ctcgtcgaag agccgcatcc ccttgcgtac ggcgatcggg tgcaccagca gcgccagctc
     6961 gtcgagcagg ccggcggcca gcagctgacg cacgaccgag accgagccgc tcagcgcgat
     7021 gtccccgccc ggctcgttct tgagggcggt gaccgcgtcc acgaggtcgc cctgcagctg
     7081 ctcggagttg cgccaaccga ggtcgagctg ctggtgcgac accacgatct tgcgcatgtc
     7141 gccgagcttc ttggcgaagt cggcgtcctc gccgccggcc tcctcccgct ctggccacgc
     7201 cccggcgaag ctgtcgtagg tcttgcggcc gaacagcagg gtgtcggccg cgccgatgct
     7261 ggcgtcgacc gccgcgccca tctcgtcgtt gaagtaggga aagtgccact ggtcgggggc
     7321 ctccaccacc ccgtcgaggg agatgaacag gccggcagtg atcgtgcgca tgggtggtcc
     7381 tccacggacg agcggtgggg gctacccggc cagcctaggc acgccgcgca ccggtcacgg
     7441 cggcatcacg caagcgcgat cagccctcgt tgatcacgag gtcgagcgcc cagacccgac
     7501 cggcgcgggc cggcgcggtg agctcggcgg tccagcgagc gcccgccagc tcggccagct
     7561 cggccggggt ccgcggggct gtcggcgcct gcaccagctc gcgggccagc tcgccgcggg
     7621 tgtgcttggc catgtggctg accacggtgc gccggcctgc gtgctcgcgc agcacgcgta
     7681 cggcgacggt gcgttcggcc agcgcgggcg gtgggaccca ggccgcggcg tacgtcgagg
     7741 accgcaggtc caccaggagc ccgcggccaa tgccggcggg cagcacgcgc gacagcggct
     7801 ctcgccaggc ggcggccagc ggaccgatgc ccggcaggtc ggcgttcatc gcgagccggt
     7861 agcgcggcac ccggtcgccg agcctcagcg ccccgaacag cgcgctgacc acgaccaggt
     7921 tgcgggtggc ccgccgccgg gccggcgcgt cgagcgaggc caggtcgagc gcgtcgtaca
     7981 gcactccggt gtaaagccgg gccaccggaa gcgtggcggc cgtctcgagg tcggcgttgc
     8041 gcgccacctc gccgaccagc ccgggggaga gcccgagtcg ccgtacggcg tccgcgggct
     8101 ggctgcggca cacgtgcaca agggagtcga gaacggcgtg acgcgtggcg gtcagctcgg
     8161 ggaacgacag cccgcacagg tccagcgtcc gcccccggga ggcgctcgcc ttgccctcgg
     8221 acggcggcag caggacgagc acggcgggtc agcctacggg ggtggctcgg ccggtctgcg
     8281 taggctggct cgatgcctcg ccgactgctg cgactgcgtg cggacggtgg cctgctgcga
     8341 gcccggttcg ccgcgatccg ggacgagctc ggggtccccg acgggttctc ctcgccggtg
     8401 ctcgccgagg ccgaggcggc gacgagcgcc gcggcgggtg tgtcaggtgt gccgggcggg
     8461 ccggctgcgc cggaggaccg caccgacctg ccgctgctga ccatcgaccc gccgggctcg
     8521 atggatctcg accaggccct gcacctgcag cgacacgggc gcggttaccg ggtgcactac
     8581 gcgatcgccg acgtggcgac cttcgtccgg cccggcgggg ccatcgatgc ggaggcccac
     8641 cgtcgcgccg agacgatcta cgcgcccgac gaacgggccc cgctgcaccc accggcgctg
     8701 tcagagggtg cggccagcct gctgcccggg caggtgcgcc cggcggtgct gtggacgatc
     8761 gacctcgacg ccgacggcga gctgatcggc gccgacctgc gccgcgccct ggtgcgcagc
     8821 cgccagcgct gggactacga ggcggtccag cgcggcgtcg acgccggcga cgagcggtgg
     8881 gcgctgctgg gcgagatcgg gcggctgcgg ctggcccgcg aggtcgagcg tggcggcgtc
     8941 gacctgccga tccctgagca ggaggtggtc cccagcggcg ccggcttcgc gctggcctac
     9001 cgcacggtcg tgccggccga gcggtggaac gcgcagatct cgctgctcac ggggatcgcg
     9061 gcggcccggc tcatggtggc cgggcgggtc ggcgtgctcc ggaccctgcc ggcggccccc
     9121 aacgggcgca ccgagctgct gcgccgtacg gcgagtgcac tgggcatcga ctggccggcc
     9181 ggggacacct tcgccgagct cgtgcactcg ctcgacccgg cccggcccga ggtggccgcg
     9241 ttcgtgcagg aggccacctc gctgctgcgc ggggccggct acgtcagctt tgacggcgag
     9301 ctgccacccg agcacgagca cgcggcggtg gcctcggtct acgcccacgt caccgcgccg
     9361 ctgcgccggc tggtcgaccg ctatgccacc gagtgctgcc tcgcgctggt cgaagggcgc
     9421 gacgtgcccg agtgggtccg ggcccgcctg gccgagctgc ccaagacgat ggccgtcggt
     9481 gaccagctgg cggcccgcct cgaacgggcc tgcctcgacg cggtcgaggc agcggtgctg
     9541 gccggcgcgg tcgggcgggt gttccccgcg gtggtggtcg aggtccgcga gggtggcggg
     9601 gtggtgcagc tgcgcgagcc ggccgtgcgg gcccgctgcg acggcgaggc gctgcccctg
     9661 ggtgagctgg tcgacgcgcg cctggtggag gccgacgtac agaccgggac cgtccggttc
     9721 gcgctggcat aggggccctg gcctgatcgc tacccttggg gtacggcgga cgagccggcc
     9781 gggcggtcgc gtcgggtgcc cctcggggcg ctcgccgagg aaagtccggg ctccacaggg
     9841 cagggtggtg gctaacggcc acccggggtg acccgcggga cagtgccaca gaaagcagac
     9901 cgccgacggc tcggctcgcc gggtcgccgg taagggtgaa acggcggtgt aagagaccac
     9961 cagcgcccgg ggtgacccgg gcggctcggc aaaccccacc cggagcaagg tcagacaggg
    10021 agcgatgagg gcggcccgcc cgagctcccg ggtaggccgc tggaggccga cggcaacgtc
    10081 agcccgagat ggatggccgc ccccggcgcc tcggcgtcgg gacagaaccc ggcttacagg
    10141 ccgactcgtc cgccgcctgc gctacgtggc cccggccatg tccgatctgc ggctttggta
    10201 gagttgcaag tcggtcacat accaaagctg agcgctggcg gctgggcagg acctctgcta
    10261 cggtcagcct caggttgttc atgcagttct cgatcgttcg tttcgacgcc cgcgagacac
    10321 accagtctcc gggcgtttgt gctcagtggg atcggttccc gggggcgggc ggcggccagt
    10381 acggcgacaa ccattcgggt cggcaaggtg ccggaccggg accgatcctc acgaggagag
    10441 cacgacatgg cacagggcac tgtgaagtgg ttcaacgacg agaagggcta cggcttcatc
    10501 tccgtcgacg acggcagcaa ggatgtcttc gtccacttct ccgccatctc aggcgagggt
    10561 ttccgctcgc tgcaggaggc ccagcgggtg gagttcgaca tcacccaggg gccgaagggt
    10621 ccgcaggcgg agaacgtccg cccgctctga cccgaccggg tgcctgacag gcaccccctc
    10681 gagccaccac acgaaggacc ctggcccaca cgggccaggg tccttcgtct tgttgtcggt
    10741 aggtcggtgt ggtcaggcga gggtgaggat ctccgccccg gtctcggtga cgagcaacgt
    10801 gtgctcgaac tgcgccgtac gcctgcggtc gcgggtcacg acggtccagc cgtcgtccca
    10861 catctcccag tcggcgctgc ccagcgtgag catcggctcg atcgtgaacg tcatgccggc
    10921 ctcgatcacg gtgtcgtagg ccgggtcgtc gtagtgcggg atgatcagcc ccgagtggaa
    10981 cgccgagccg atcccgtgcc cggtgaagtc gcggacgacg ccatagccga accgccgcgc
    11041 gtaggcctcg atgacccggc cgatgacgtt gacctgccgg cccggcgcga ccgccttgat
    11101 cccgcgcatc atcgcctcgt gggtgcgctc gacgagcagc cgagaggcct cgtcgacctc
    11161 gccgaccagg taggtggcgt cggtgtcgcc gtgcaccccg tcgatgaagg cggtgatgtc
    11221 gacgttgacg atgtcgccgt cgcgcagctc ggtcgagtcg gggatgccgt ggcagatcac
    11281 ttcgttgacc gacgtgcaca gcgacttggg aaagccccgg tagcccagcg tcgacgggta
    11341 ggcgccgtgg tcgcagagga actcgtgccc gacccggtcg agctcgtcgg tgctcacgcc
    11401 gggggcgacg tgggtggaca cctcgaccag cgcggccgcc gcgatctgac cggccacccg
    11461 catcagctcg atcgtctcgg cgtccttgac ctccggcccg tcgtacggcg caggagctgg
    11521 gtgtccgacg taggacgggc gtcggatgtg ctgcgggacg ggtcgggtcg gtgagatcgt
    11581 gccggggatg agcagggcca tgactggcca gtctgccagg ccgtcccggc cgccgagatc
    11641 gaggagcacg cgttgtccga ccgctggtac tactgcctca agcacgacac cgtcgagccg
    11701 gaggccggct gcgccaacaa ggaccggctg ggcccctacg ccacccgcga ggaggccgcc
    11761 gacgcgctgg ccctggccgc gcggcgcaac gaggcatggg ccgccgccga cgacgaggac
    11821 gacgacgact gagcgcgccg gccttcgtgg cgatcatgga tttctccatg atcgtgtcaa
    11881 tccgtccagg tgctagggct gcaatcttcc acgatcgtgc ccagacgacg ccgactgccc
    11941 tgacgccgcg cggtagtgat ccccgactcg acggtcgggt caccagcgag gccggctgcc
    12001 ttcgggggcg gggccgctga cggcgtcgag gaagcgcgag agccgctcgc gcagcccccg
    12061 gcggccggcc cgatcgggct ccccggcggc cgtgctgacc aggtgctgca ccgtggccat
    12121 cgagaccagc gacccgccgt cgtccggggc aggcgctggc tct
//
LOCUS       DATIBB010000147         2212 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_1536066, whole genome shotgun sequence.
ACCESSION   DATIBB010000147 DATIBB010000000
VERSION     DATIBB010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2212
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1536066"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1198
                     /gene="radA"
                     /locus_tag="VLC50_05725"
     CDS             <1..1198
                     /gene="radA"
                     /locus_tag="VLC50_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013772644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HSF26999.1"
                     /translation="PARPIGEIALDAAKARPTGVGELDRVLGGGLVPGAVVLLAGEPG
                     VGKSTLALDVAARAGRHGTVLYVSGEESAAQVRLRAERLGAVHERLYLAAETDLGAVL
                     GHIEALDPTLLVVDSVQTIASAEVDGAAGNVAQVREVASALIRAAKTRGCPTILIGHV
                     TKDGSVAGPRVLEHLVDVVLAFEGERHSRLRLVRSTKNRFGATDEVGCFDLGDAGLVE
                     LADPSELFRTRHSEATPGSAVTVALEGRRPLVAEVQALVAPSPIANPRRAVSGLDSAR
                     ASMILAVLQRRAMLKLADHESYLATVGGVRLSEPAADLAVALAVTSARVDRPIPTEVI
                     TFGEVGLAGEIRRVAGMGRRLAEAARLGFQVAIVPPEPGPAPAGLTVIEVGDIVSAIM
                     AARLIG"
     gene            1284..>2212
                     /gene="disA"
                     /locus_tag="VLC50_05730"
     CDS             1284..>2212
                     /gene="disA"
                     /locus_tag="VLC50_05730"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007466050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA integrity scanning diadenylate cyclase DisA"
                     /protein_id="HSF27000.1"
                     /translation="MHAAPIGGSVAASDKSADDLLRATLAAVAPGTELRDGLERILRG
                     NTGALIVLGWDRTVESLCTGGFVLDVEFAATRLRELCKMDGAVVVDAEGSMRIVRAGV
                     QLVPDPSIPTDETGTRHRTAVRVARQTGFPVISVSQSMRIVAIYVRERRYVLEDSGQI
                     LSRANQALATLERYKLRLDEVSGTLSALEIEDLVTVRDVASVAQRLEMVRRIAMEIEG
                     YVVELGTDGRLLSLQLDELVAGVEPDRELVVRDYLAPASGRKARTVADVLRDLDTLSA
                     AELVDLPAVARAIGFTTETLESAVSPRGHRLLAR"
BASE COUNT          275 a          829 c          817 g          291 t
ORIGIN      
        1 gccggcccgc ccgatcggtg agatcgcgct cgacgcggcc aaggctcgcc cgaccggcgt
       61 gggtgagctc gaccgggtgc tcggcggcgg gctggtgccc ggcgcggtgg tgctgctggc
      121 cggtgagccc ggggtgggca agtcgacgct ggccctcgac gtcgcggccc gagccggccg
      181 gcacgggacc gtgctctacg tcagcggcga ggagtcggcg gcccaggtgc ggctgcgggc
      241 cgagcgcctc ggcgcggtgc acgagcggct ctacctggcc gccgagaccg acctgggcgc
      301 ggtgctcggg cacatcgagg cgctcgaccc gacgctgctg gtggtcgact cggtgcagac
      361 catcgccagc gccgaggtgg acggggccgc cggcaacgtg gcccaggtcc gcgaggtggc
      421 ctcggccttg atccgggccg ccaagacccg cggctgcccg accatcctga tcgggcacgt
      481 caccaaggac ggctcggtgg ccgggccccg ggtgctcgag cacctcgtcg acgtggtgct
      541 ggcgttcgag ggcgagcggc actcgcggct gcggctggtc cgctcgacca agaaccgttt
      601 cggggccacc gacgaggtcg gctgcttcga cctgggcgac gccgggctgg tcgagctggc
      661 cgaccccagc gagctgttcc gcacccgcca cagcgaggcc actcccggca gcgcggtgac
      721 cgtggccctg gaggggcgcc gaccgctggt cgccgaggtg caagccctcg tcgcgcccag
      781 cccgatcgcc aacccgcgcc gagcggtcag cgggctcgac tcggcccggg cgtcgatgat
      841 cctcgcggtg ctgcagcgcc gggcgatgct caagctggcc gaccacgaga gctacctggc
      901 caccgtcggc ggggtcaggc tgtccgagcc ggccgccgac ctcgcggtcg cactggcggt
      961 gaccagcgcg cgtgtcgacc ggcccatccc caccgaggtg atcacctttg gcgaggtcgg
     1021 gctggccggg gagatccgcc gggtcgccgg catgggccgc cggctggccg aggcggcccg
     1081 gctcgggttc caggtcgcga tcgtgccgcc cgagccgggc ccggccccgg ccggcctcac
     1141 ggtgatcgag gtcggcgaca tcgtcagcgc gatcatggcg gcccggctga tcggctgatc
     1201 ggctgacggc caccgatggc gcacacgcac cccgagtgac catgccggct cgccgttaga
     1261 ctccggggca atctctccgc cacctgcacg ccgctccgat cgggggttcc gtggccgcca
     1321 gcgacaagag cgccgacgac ctgttgcgcg cgaccctggc cgccgtcgcc ccgggcaccg
     1381 agctgcgcga cgggctcgag cgcatcctgc gcggcaacac cggcgcgctg atcgtgctgg
     1441 gctgggaccg caccgtcgag tcgctgtgca ccggcgggtt cgtcctcgac gtcgagttcg
     1501 ccgccacccg gctgcgcgag ctctgcaaga tggacggcgc ggtggtcgtc gacgccgagg
     1561 gcagcatgcg catcgtgcgc gccggcgtac agctcgtgcc cgacccgtcg atcccgaccg
     1621 acgagacggg cacccgccac cgcaccgcag tgcgggtggc ccgccagacc ggcttcccgg
     1681 tgatctcggt cagccagtcg atgcgcatcg tcgcgatcta tgtgcgggag cggcgctacg
     1741 tcctcgagga cagcggccag atcctctccc gggccaacca ggccctggcc accctcgagc
     1801 gctacaagct gcgcctcgac gaggtgtccg gcacgctgtc cgcgctcgag atcgaagacc
     1861 tcgtcaccgt ccgcgacgtc gcctcggtgg cccagcgcct cgagatggtc cggcgcatcg
     1921 cgatggagat cgagggctac gtcgtcgagc tcggcaccga cggccggctg ctctcgctgc
     1981 agctggacga gctggtcgcc ggcgtggagc ccgaccgcga gctcgtcgta cgcgactatc
     2041 tggccccggc cagcggacgc aaggcccgca ccgtggccga cgtgctgcgc gacctcgaca
     2101 cgctctcggc cgccgagctg gtcgacctgc ccgcggtcgc ccgagccatc gggttcacca
     2161 ccgagacgct cgagtcggcg gtcagcccgc gcgggcaccg gctgctcgcc cg
//
LOCUS       DATIBB010000148         5169 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_485087, whole genome shotgun sequence.
ACCESSION   DATIBB010000148 DATIBB010000000
VERSION     DATIBB010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 5169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5169
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_485087"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3448
                     /locus_tag="VLC50_05735"
     CDS             <1..3448
                     /locus_tag="VLC50_05735"
                     /EC_number="4.1.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="multifunctional oxoglutarate
                     decarboxylase/oxoglutarate dehydrogenase thiamine
                     pyrophosphate-binding subunit/dihydrolipoyllysine-residue
                     succinyltransferase subunit"
                     /protein_id="HSF27001.1"
                     /translation="ASSAAPARTSAPARTSAPARTSAPATSPEPPSWTAATVHPIKGS
                     AARVVTNMDASLTLPTATSVRAIPAKLLVDNRIVVNNHLKRGRGGKVSFTHLIGFALI
                     KALESMPEMNNGFTEVDGKPALRQPAHVNLGIAIDLSKPDGSRTLLVPNVRGAEAMDF
                     AQFWSAYEDVVRRARAGKLTADDFAGTTISLTNPGTIGTVHSVPRLMSGQGTIIGVGA
                     MEYPAEYQGASAETLARLAVSKVVTLTSTYDHRIIQGAQSGDFLRRIHQLLLGADGFY
                     DEIFRALRIPYEPIRWIQDISATHENDVVKQARVQELIHSYRVRGHLMADTDPLEFKQ
                     RHHPDLDVVTHGLTLWDLDREFATGGFGGKPMMALRDILGVLRDSYCRRVGIEYMHIQ
                     DREERTWIQARVERRHDKPSREEQLRILRRLNAAEAFETFLQTKYVGQKRFSLEGGES
                     VIPLLDHVLTAAAREQIDEVCIGMAHRGRLNVLANLAGKSYGQIFREFEGNLDPRSVQ
                     GSGDVKYHLGTQGTFTAETGEQCKVYLAANPSHLEAVDPVLEGIVRAKQDKINKGGGE
                     EGFTVLPILVHGDAAFAGQGVVAETLNLSQLRGYRTGGTIHVVINNQVGFTTPPTSSR
                     SSFYATDVARMIQAPILHVNGDDPEACVRVAALAFEFRQTFKKDVVIDMVCYRRRGHN
                     EGDDPSFTQPRMYSLIEAKRSVRKLYTEALIGRGDITVEEAEQALRDYQGQLERVFVE
                     TRAASDSPLSLEPPVDEAAAPEHPLAPVSFDVLKRIADAHLAVPEGFTVHPKVLPQVQ
                     RRAGMITGGGIDWSTGEAIAFGSLLLEGVPVRLAGQDTRRGTFSQRHAVLIDRLTADE
                     WTPLAHLSDDQARFWVYDSLLSEYAALGFEYGYSAERTDALVVWEAQFGDFVNGAQSI
                     IDEFISSSQQKWGQRSGVVLLLPHGYEGQGPDHSSGRPERFLQLAAEDNMTVAMPSTP
                     ANYAHLLRRQAHRSPRRPLVVFSPKSMLRLKAAVSSVADFTDQGFRPVIADAEVTDPA
                     AVTRVLLCAGKVYYDLAAARARREDTSTAIVRLEQLYPLPARALREALAPYGDAELVW
                     VQEEPANQGPWPFMALNLPEQLGGRSLRPVSRPAAASPAVGSHKQHELEQRELVERAF
                     AR"
     gene            complement(3512..4477)
                     /locus_tag="VLC50_05740"
     CDS             complement(3512..4477)
                     /locus_tag="VLC50_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011291717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HSF27002.1"
                     /translation="MFAVYAQSLSDTDPLSGLVVGERPDPEVPDGWARVRVRAAALNH
                     HDLWSLRGVGLPAERLPMILGCDAAGVDDEGHEVVVHAVVTDPAWRGDDTLDPKRSLL
                     SERYQGTFADTVVVPRRNLVPKPAGLSFEQAACLPTAWLTAYRMIFTQSGVAPGGTLL
                     VQGAGGGVASALLMIGHAAGYRVWVTSRDAAKRARALELGADAAFEPGARLPERVDAV
                     METVGAATWSHSLKALRPGGTLVVAGATSGANPPAELNRVFFLQLRVVGSTMGTRDEL
                     QRVLQLCEQTGVRPLIDEVFPMERARDALARMAEGDVFGKLVLTR"
     gene            complement(4506..>5169)
                     /locus_tag="VLC50_05745"
     CDS             complement(4506..>5169)
                     /locus_tag="VLC50_05745"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003997977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0004473 - malate dehydrogenase
                     (decarboxylating) (NADP+) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HSF27003.1"
                     /translation="LAALRNAARLTDRELADVRVVVSGAGAAGVAITKFLQSAGVGDV
                     VVVDSRGIIHRDRADLSPVKRDLAATTNEAGRTGTITDALRGADALVGVSGGTIPEAA
                     VAAMARDCFVFAMANPTPEVHPDIAASHASVVATGRSDFPNQINNVLAFPGVFRGALQ
                     VRATCVTEGMKHAAAQALADLVGDDLTEELVIPSVFDPRVGPAVAEAVAAAARRDGVA
                     RV"
BASE COUNT          768 a         1956 c         1774 g          671 t
ORIGIN      
        1 ggccagcagc gcggcaccgg cccgcaccag cgccccggcc cgcacgagcg caccggcgcg
       61 caccagcgcc ccggcgacca gccccgagcc gccgtcgtgg acggccgcca cggtccaccc
      121 gatcaagggc agcgcggccc gggtggtcac caacatggac gccagcctga cgctgcccac
      181 cgcgaccagc gtgcgggcca tcccggccaa gctgctcgtc gacaaccgca tcgtggtcaa
      241 caaccacctc aagcgcggcc gcggcggcaa ggtctcgttc acccacctca tcggcttcgc
      301 gctcatcaag gcgctcgagt cgatgccgga gatgaacaac ggcttcaccg aggtcgacgg
      361 caagccggcg ctgcgccagc ccgcgcacgt caacctcggc atcgcgatcg acctgtccaa
      421 gcccgacggc agccgcaccc tgctggtgcc caacgtccgc ggtgctgagg cgatggactt
      481 cgcgcagttc tggtcggcct acgaggacgt cgtacgccgg gcccgggcag gcaagctgac
      541 cgccgacgac ttcgccggca ccacgatcag cctcaccaac cccggcacca tcggcaccgt
      601 ccactcggtg ccccggctga tgtccgggca gggcacgatc atcggtgtcg gggcgatgga
      661 gtacccggcc gagtaccagg gtgcctcggc cgagacgctg gcccggctgg cggtcagcaa
      721 ggtcgtcacg ctgacctcca cctatgacca ccggatcatc cagggcgccc agtccggcga
      781 cttcctgcgc cgcatccacc agctgctgct cggcgccgac ggcttctacg acgagatctt
      841 ccgcgcgctg cgcatcccct acgagccgat ccgctggatc caggacatct cggccaccca
      901 cgagaacgac gtggtcaagc aggcccgggt gcaggagctc atccactcct accgggtccg
      961 cgggcacctc atggccgaca ccgacccgct ggagttcaag cagcgccacc accccgacct
     1021 cgacgtggtc acccacgggc tgacgctgtg ggacctcgac cgcgagttcg ccaccggcgg
     1081 cttcggcggc aagccgatga tggcgctgcg cgacatcctc ggcgtgctgc gcgactccta
     1141 ctgccgccgg gtcggcatcg agtacatgca catccaggac cgcgaggagc gcacctggat
     1201 ccaggcgcgc gtcgagcgcc gccacgacaa gccctcccgc gaggagcagc tgcgcatcct
     1261 gcgccggctc aacgcggccg aggcgttcga gaccttcctg cagaccaagt acgtcggcca
     1321 gaagcgcttc tcgctcgagg gcggcgagtc ggtcatcccg ctgctcgacc acgtcctcac
     1381 cgcggcggcc cgcgagcaga tcgacgaggt gtgcatcggg atggcccacc gcggccggct
     1441 caacgtgctg gccaacctcg ccggcaagag ctatggccag atcttccggg agttcgaggg
     1501 caacctcgac ccgcgatccg tgcagggctc cggtgacgtg aagtaccacc taggcaccca
     1561 gggcacgttc accgccgaga ccggcgagca gtgcaaggtc tacctggccg ccaacccctc
     1621 gcacctcgag gcggtcgacc cggtactcga gggcatcgtc cgggccaagc aggacaagat
     1681 caacaagggc ggcggcgaag aggggttcac cgtcctgccg atcctggtcc acggcgacgc
     1741 ggcgttcgcc ggccaggggg tcgtcgccga gacgctcaac ctgtcgcagc tgcgcggcta
     1801 ccgcaccggc ggcacgatcc acgtggtgat caacaaccag gtcgggttca ccacgccgcc
     1861 aacctcgtcg cgctcgtcgt tctacgcgac cgacgtcgcc cggatgatcc aggcgccgat
     1921 cctgcacgtc aacggcgacg accccgaggc ctgcgtgcgc gtcgcggcgc tggccttcga
     1981 gttccgccag acgttcaaga aggacgtcgt catcgacatg gtgtgctacc gccggcgcgg
     2041 gcacaacgag ggcgacgacc cgtcgttcac ccagccgcgg atgtactcgc tgatcgaggc
     2101 caagcgctcg gtgcgcaagc tctacaccga ggcgctgatc gggcggggcg acatcacggt
     2161 cgaggaggcc gagcaggcgc tgcgcgacta ccagggccag ctcgagcggg tcttcgtgga
     2221 gacgcgggcc gcctccgact caccgctgag cctcgagccg ccggtcgacg aggccgcggc
     2281 tcccgagcac ccgctcgcgc cggtgtcctt cgacgtgctc aagcgcatcg ccgacgccca
     2341 cctcgccgta cccgaggggt tcaccgtcca ccccaaggtg ctcccgcagg tccagcgccg
     2401 ggccggcatg atcaccgggg gtgggatcga ctggtcgacc ggcgaggcga tcgcgttcgg
     2461 gtcgttgctg ctcgaaggcg tgccggtgcg gctggccggc caggacaccc ggcgcggcac
     2521 gttctcgcag cgccacgcgg tgctcatcga ccggctcacc gccgacgagt ggacccccct
     2581 cgcccacctg tccgacgacc aggcccggtt ctgggtctac gactcgctgc tgtccgaata
     2641 tgccgcgctc gggttcgagt acggctactc cgccgagcgc accgatgcgc tcgtcgtgtg
     2701 ggaggcccag ttcggcgact tcgtcaacgg cgcccagtcg atcatcgacg agttcatctc
     2761 ctccagccag cagaagtggg gccagcgctc cggcgtcgtc ctgctgctgc cgcacggcta
     2821 cgaggggcag gggcccgacc actcctccgg ccggccggag cggttcctgc agctggccgc
     2881 cgaggacaac atgaccgtcg cgatgccgag cacgccggcc aactacgccc acctgctgcg
     2941 gcggcaggcc caccggtccc cgcgccggcc gctcgtggtg ttcagcccca agtcgatgct
     3001 gcggctgaag gccgcggtct cgtcggtggc cgacttcacc gaccaggggt tccgtccggt
     3061 gatcgccgac gccgaggtca ccgaccccgc ggccgtcacc cgggtgctgc tgtgcgcggg
     3121 caaggtctac tacgacctag ccgcggcccg ggcccgacgc gaggacacct cgacggccat
     3181 cgtgcggctc gagcagctct atccgctgcc ggcccgggcg ctgcgtgagg cgctcgcgcc
     3241 ctacggtgac gccgagctgg tctgggtgca ggaggagccg gccaaccagg ggccgtggcc
     3301 gttcatggcg ctcaaccttc ccgagcagct cggtgggcgc tcgctccgcc cggtctcccg
     3361 gccggcggcc gcctcgccgg cggtcggctc gcacaagcag cacgagctcg agcagcgcga
     3421 gctggtcgag cgggccttcg cccgctgacc gcgggggtcg gcaccgcagg aaccgccggc
     3481 tcgcggtctc gtcgaccgcg ggccggcagg gtcagcgggt caggaccagc ttgccgaaga
     3541 cgtcgccctc ggccatccgg gcgagggcgt ctcgggcgcg ctccatcggg aacacctcgt
     3601 cgatgagcgg gcgcacgccg gtctgctcgc agagctgcag cacccgttgg agctcgtcgc
     3661 gggtgcccat cgtcgagccg accacccgca actgaaggaa gaacacccgg ttgagctcgg
     3721 cgggcgggtt ggccccgctg gtcgcccccg cgacgacgag cgtgccgccc gggcgcaacg
     3781 ccttgagcga gtggctccag gtcgcggcgc cgaccgtctc catgaccgcg tcgacccgct
     3841 cgggcagcct ggcgcccggc tcgaacgccg cgtcggcgcc cagctcgagg gcgcgggccc
     3901 gcttggccgc gtcacggctg gtcacccaga cccgatagcc ggccgcgtgc ccgatcatga
     3961 gcagcgccga ggccaccccg cccccggcac cctgcacgag cagcgtgccc cccggcgcca
     4021 cccccgactg cgtgaagatc atccggtagg cggtcagcca ggcggtcggc aggcaggcgg
     4081 cctgctcgaa ggacagcccg gccggcttgg gcaccaggtt gcgccggggt acgacgaccg
     4141 tgtcggcgaa cgtgccctgg tagcgctcgg acagcagcga ccgcttgggg tcgagcgtgt
     4201 cgtcgccgcg ccaggccggg tcggtgacca ccgcatggac gacgacctcg tgaccctcgt
     4261 cgtcgacgcc cgccgcgtcg cagccgagga tcatcggcag gcgctcggcc ggcaggccca
     4321 ccccgcgcaa cgaccacagg tcatggtggt tgagcgcagc cgcccggacc cgcacccggg
     4381 cccagccgtc gggcacctcc gggtcgggcc gctcccccac gaccaggccg ctcagcgggt
     4441 cggtgtcgga caggctctgg gcatagacgg caaacacgca gcgaccctag cggtcgcctc
     4501 ggtgctcaga cgcgggcgac cccgtctcgt cgagcggccg cggccaccgc ctcggcaacg
     4561 gccggcccca cccgcgggtc gaacacgctg gggatcacca gctcctcggt gaggtcgtcg
     4621 ccgacgaggt cggccagcgc ctgtgccgcg gcgtgcttca tgccctcggt cacgcaggtc
     4681 gcgcgcacct gcagggcgcc ccggaagacg cccgggaagg ccaacacgtt gttgatctgg
     4741 ttggggaagt cgctgcgccc ggtagcgacc accgaggcgt gcgaggccgc gatgtccgga
     4801 tggacctcgg gcgtggggtt ggccatcgcg aagacgaagc agtcgcgggc catcgccgcc
     4861 accgccgcct ccgggatggt cccgccggag accccgacca gggcgtcggc gccgcgcagg
     4921 gcgtcggtga tcgtgccggt gcggccggcc tcgttggtgg tggccgcaag gtcacgcttg
     4981 accgggctga ggtcggcccg atcgcggtga atgatcccgc gcgagtcgac caccacgacg
     5041 tcgccgacgc cggccgactg caggaacttg gtgatggcca ctcccgcggc gccggccccc
     5101 gacacgacga cgcgcacgtc ggccagctcg cggtcggtca gccgggcggc gttgcgcagc
     5161 gcggccagc
//
LOCUS       DATIBB010000149        10294 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_223183, whole genome shotgun sequence.
ACCESSION   DATIBB010000149 DATIBB010000000
VERSION     DATIBB010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 10294)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10294)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10294
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_223183"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(65..2455)
                     /locus_tag="VLC50_05750"
     CDS             complement(65..2455)
                     /locus_tag="VLC50_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HSF27004.1"
                     /translation="MQTQRYSGTSVRRVEDRRLITGNGRYVDDLAPANVAHAAIVRSP
                     FAHARIRRIDVEAARAVPGVIAVLTAAELLPHVHAPFPVIEAGVTNPRQSPPQYPIAR
                     DEAVYEGEPLAVVVAETRYAAADGAALVELDLEALPAVVDLDAALEPGAPTVREGAPD
                     NVAWDVVYPGTGDIDAAFAEAAVVVRERILQQRVAAAPMEGRAVLADFAGSDDELTLW
                     TGSQMPHFIRMYVAEGLGVPESKLRVISPDVGGAFGAKMGPYAEEYLLPIASKLTGRP
                     VKWSETRSENLAVTSHGRGHTFDVEAAAAADGTLLGLRIVQHTDVGAYIGRAGANAVI
                     AIFLAGGCYRWGAIEGHAIGVLTNKMWTGPYRGAGRPEATHLCERVLDLLAAKLEMDP
                     AELRRRNFIREFPFANQFGFTYDSGDYDGALTRALELVDYDGFRREQEQARREGRYLG
                     IGIGSYVEIAGFGPSGMTAANAGEIGLVESAVVRVHPTGSVSVSAGTHSHGQGHATAF
                     AQIVADALGVPMDAVKVTEGDTANTPFGHGTYGSRSIPVGGTAVFTACQKVVAQATRL
                     AAHLLEAAEADVEHVDGGFRIRGTDVAKTFNEVAFAAYNKDLPEGMEQGLEAVAFFDP
                     PNFTWPFGTHVCLVEVDPKTGVTSINRYVAVDDCGNVINPMIVEGQVHGGVLQGVAQA
                     LLEEVTYDPSSGQRIGGTFLDYLVPTINEKVPTEVHHTVTPTPSNVLGAKGVGEAGTI
                     ASSVAVINAICDALSPFGIRHVDMPASPARIWGMLHDAPSPSAPSPERAAAPTALH"
     gene            complement(2516..3328)
                     /locus_tag="VLC50_05755"
     CDS             complement(2516..3328)
                     /locus_tag="VLC50_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020422716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HSF27005.1"
                     /translation="MIPSTFDYVRATDLAAAAEAVRAGGEDAKVIAGGMSLIPLMKLR
                     LAEPVLLVDLAGLDELRYIKVEDGRLRLGALTRHVDIARSPVVASALPLLAQVAGEVG
                     DAQVRARGTIGGVLAHADSAGDYCTVAMMLDAEIVTTRNRYAATDFLLDFMTTPLEPD
                     ELITEVSFPVLDGPHDYVKFRRRRADWAIVGVGVQAGPDGWRIGLTNVASTALRARAG
                     EEALAGGASAAEAAAVVAESVHPSDDLATPAAYREDLVRTLTERALNSVGVS"
     gene            complement(3330..3824)
                     /locus_tag="VLC50_05760"
     CDS             complement(3330..3824)
                     /locus_tag="VLC50_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012876164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HSF27006.1"
                     /translation="MSSPELPTHVVTIEVNGRPRTDVVEPRTLLVHHLRDRLALTGTH
                     VGCDTSQCGACTVLLDGEAVKSCTIFAVQADGRSVTTVEGLATAEGLHPVQEAFREEH
                     ALQCGYCTPGFMMSTVSLLASNPDPTADEIGVGLEGNICRCTGYVNIEKAVLAAAATY
                     QMGR"
     gene            complement(3881..4924)
                     /locus_tag="VLC50_05765"
     CDS             complement(3881..4924)
                     /locus_tag="VLC50_05765"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSF27007.1"
                     /translation="MTNADAAERTDVTTDSVAPSVATAARTSGGPGRMVMVLQRYGLL
                     LALLLAVVVFSLLRPSSFATTQNLASILTGSAALTLISLGIMMPLIVQQFDLSVGYTA
                     TIASLLTVGVLSRNDWPVWQAIALGLLVSLVIGVVNGLLVAYAGLNSLVVTLGVGSAL
                     YGLSLLYSGGQLISANIPPSFKHLGQARPFGVPLAFIYALVAGVLIWYFLSYRPTGRR
                     MYAVGGSETAARLAGIRVPFLVLMTFVMSALLCGLGGIVQSARVGSSSADSMTSFLLP
                     AFTAAFLGATAIRPGHYNVWGTFLAVYLVATGTTGMFMLGAQTYVEPVFNGLILLVAI
                     AVARMTMRHERKA"
     gene            complement(4953..6521)
                     /locus_tag="VLC50_05770"
     CDS             complement(4953..6521)
                     /locus_tag="VLC50_05770"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HSF27008.1"
                     /translation="MTVEPVLALQTSDVSKHFGATQALRSVDFHVRKASVHALLGRNG
                     SGKSTLIKVLSGFHQADGGQVVVDGTELPPHHNPRQAHAAGLRFVHQDLGLVAGLSVA
                     ENLTLDVPFKTDGTGTIRWRRQIVSAAAELGRLGLHLDPRRTVATLGPVEQTMVAVAR
                     ALQGLDYAHSVLVLDEPTARLPRSEVDRLLAILGTLKSQGVAIVYVTHRLDEVFAAAD
                     EVTILRDGAQVFTGPVSETDEAAVSRYIIGRITVSEAPAINRDETSARTGQPVLELRG
                     ISGRRVHDVSMAIHPGEVVAVTGLVGSGRSELGRLVYGIEALTAGEVRLHGHPMTRLT
                     PRHASAAGMGYSPQERGDGLAVGLPIMENLAVTSYDGLTHAVGVSVHRLQAMAKRVIQ
                     ELAVAPPEPGAVVNTLSGGNQQKVSLGKWTRKNLVLLVLDEPLQGIDVGAKAEIMRAI
                     RQRAQDDGLAVLWLESDIEEVPKYADRVLVMRDGRISTELAPSDVSKAGLLAAVYDRS
                     SAQGTGATAAGGVK"
     gene            complement(6577..7695)
                     /locus_tag="VLC50_05775"
     CDS             complement(6577..7695)
                     /locus_tag="VLC50_05775"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HSF27009.1"
                     /translation="MAAAGCSSGGDSGTAADTKAPSSGSSAAAGGDSSAAVENAKKIV
                     DEWSAANSEWQGPTEAPAPKPGMRVALITCDQNAEGCARPARAQEVAAKLIGWKTTLF
                     DGQGNPAKQLQAINAAIDAKFDAIILDLVDTRVTQEGIQRAIKAGIPMVSVANLSNEP
                     ASIPDVSHDWVYAGELAAYYMIAKSEGKVNALVLLDNEFEVVKNGEYKGIMSVLEDKT
                     KCPECKVTVKEFQIANLETQPGNHAVAALQQDPSINWVWCFDACMARAATQVLASGIS
                     TEAKGIGMNANAQNLQLIRDDQFQTMTIGNPYEWEAWAALDNLNRLLNGQEVVDQKIP
                     VRIIDKSNLGELSDEELKSGWAGGIDYVAKYKELWGVK"
     gene            complement(7995..8762)
                     /locus_tag="VLC50_05780"
     CDS             complement(7995..8762)
                     /locus_tag="VLC50_05780"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HSF27010.1"
                     /translation="MSPSGVVRVERPCPGVVELVIDRPPLNVLDDQCQRELAAALRPL
                     HDEDTLLAVVVRGADDVFSVGADVKELAATTDPDPWGGVELAAHWTRSLETLPCLTIA
                     AIRGHCLGGGLEIALCTDVRIATPEARLGFPEVRLGLLPGMGGTARLPRLVGTSWATR
                     MVMTGEPISAQLGREIGLVTEVATDPRGAALTLADSLRKAGPLAARLAKSLVAQPADE
                     GALRAERLAWKCAWATADHREALAAFLAHRPAHFTGR"
     gene            complement(8759..9949)
                     /gene="lhgO"
                     /locus_tag="VLC50_05785"
     CDS             complement(8759..9949)
                     /gene="lhgO"
                     /locus_tag="VLC50_05785"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017583920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HSF27011.1"
                     /translation="MDVGIVGAGIVGLTLARQLGLSDPSCRVTILEKESAIGQHQTSH
                     NSGVVHAGLYYKPGSLKAQLCTRGRGLLREYCQANNLPFKESGKLVVALDESERAGLE
                     EIHRRATANGVPDLAWLDGAAMAEIEPHVRGYAAVHSPHTGVVDFPAVAATVAEEVVK
                     AGAGLHTDSPVTAITRRNGRIQVLAGGASHTFDRLVVCAGLQSDRVSRLGGGPADPAI
                     VPFLGMYYRLLPTRTHLVRSLIYPVPDPRYPFLGVHLTRTVHDEVLVGPNALLALSRE
                     GYRATRVDPKDVAATLRWPGFYKLAQKHWKAGARELAVAASRKAFVAAARRYVPDLGE
                     NDVERARAGVRAQAVTITGGLVDDFALVDVDGILSVRNAPSPAATSSFAIAEHLAPTV
                     LGTR"
     gene            complement(9953..>10294)
                     /locus_tag="VLC50_05790"
     CDS             complement(9953..>10294)
                     /locus_tag="VLC50_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011604270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HSF27012.1"
                     /translation="LIEVKPDTALWREEVFGPVLAATTVTDLDTAIDLVNDSDYGLSA
                     GIFTRDLSAAHQFADRVATGQVAVNLPTSGWDVHMPFGGFKDSGSLFKEQGVEGLGFY
                     TRVKTVAMRVG"
BASE COUNT         1521 a         3537 c         3656 g         1580 t
ORIGIN      
        1 cgccccccgt cttctccgca ctgatcatgc gcaaaccatc gctcggccga tggtttgcgc
       61 atgatcagtg cagggcggtt ggcgcggcgg cgcgctccgg cgacggggcg ctcgggctgg
      121 gcgcatcgtg cagcatcccc cagatgcgcg ccgggctggc cggcatgtcg acgtggcgga
      181 tcccgaacgg cgagagcgcg tcgcagatgg cgttgatgac cgcgacgctc gaggcgatcg
      241 tgcccgcctc gccgaccccc ttggcgccga gcacgttgct cggggtgggc gtcaccgtgt
      301 ggtgcacctc ggtcggcacc ttctcgttga tcgtcggtac caggtagtcg aggaacgtgc
      361 cgccgatgcg ctgaccgctg gaggggtcgt aggtgacctc ctcgagcagc gcctgtgcca
      421 ccccctgcag caccccgccg tggacctgcc cctccacgat catcgggttg atcacgttgc
      481 cgcagtcgtc cacggcgacg tagcggttga tcgaggtgac accggtcttg gggtcgacct
      541 cgaccaggca gacgtgggtg ccgaacggcc aggtgaagtt tggcgggtcg aagaacgcca
      601 cggcctcgag gccctgctcc atgccctcgg gcaggtcctt gttgtacgcc gcgaaggcca
      661 cctcgttgaa ggtcttggcc acgtccgtgc cgcggatccg gaagccgccg tcgacgtgct
      721 cgacgtcggc ctccgccgcc tcgagcaggt gcgccgccag ccgggtcgcc tgcgccacca
      781 ccttctggca ggcggtgaac accgccgtac caccgaccgg gatgctgcgg ctgccgtagg
      841 tgccgtgccc gaacggcgtg ttggccgtgt cgccctcggt gaccttgacg gcatccatcg
      901 gtacgccgag cgcgtcggcg acgatctgcg cgaatgcggt ggcgtggccc tgcccgtgcg
      961 agtgcgtgcc cgcgctgacg ctgaccgagc cggtcggatg cacgcggacg acggccgact
     1021 cgaccagccc gatctcgccg gcgttggccg cggtcatgcc gctagggccg aaaccggcga
     1081 tctcgacgta ggagccgatg ccgatgccga ggtagcggcc ctcacgacgg gcctgctcct
     1141 gctcgcggcg gaacccgtcg tagtcgacca gctcgagcgc gcgggtcagt gcgccgtcgt
     1201 agtcgccgga gtcgtaggtg aacccgaact ggttggcgaa ggggaactcg cggatgaagt
     1261 tgcgccgacg cagctcggcc gggtccatct cgagcttggc ggccagcagg tcgagcaccc
     1321 gttcgcacag gtgggtggcc tcggggcgcc cggcgccgcg gtaggggccg gtccacatct
     1381 tgttggtcag cacgccaatg gcatggccct cgatcgcgcc ccaccggtag cagccgccgg
     1441 caaggaagat cgcgatgacc gcgttggcac cggcccggcc gatgtaggcg ccgacgtcgg
     1501 tgtgctgcac gatgcgcagt ccgagcagcg tcccgtctgc ggcggcggcc gcctcgacgt
     1561 cgaaggtgtg cccgcgacca tggctggtca ccgcaaggtt ctcgctgcgg gtctccgacc
     1621 acttcaccgg gcggccggtg agcttggagg cgatcggcag caggtattcc tcggcgtagg
     1681 ggcccatctt ggcgccgaac gcgccgccca cgtcgggcga gatgacccgc agcttgctct
     1741 cgggcacgcc caggccctcg gcgacgtaca tccggatgaa gtgcggcatc tggctgccgg
     1801 tccacagcgt cagctcgtcg tcggagccgg cgaagtcggc gagcacggcc ctcccctcca
     1861 tcggggccgc ggccacccgc tgctgcagga tccgttcgcg tacgacgacc gcggcctcgg
     1921 cgaaggccgc atcgatgtcg ccggtgccgg ggtagacgac gtcccaggcc acgttgtcgg
     1981 gggcaccctc gcgcaccgtg ggggcacccg gctcgagcgc ggcgtcgagg tcgaccacgg
     2041 cgggcagcgc ctcgaggtcg agctcgacga gggccgcacc gtcggcggcg gcgtagcggg
     2101 tctcggccac caccaccgcg agcggctcgc cttcatagac ggcctcgtcg cgggcgatcg
     2161 gatactgcgg cggcgactgg cgcgggttgg tcactccggc ctcgatgacc gggaacggcg
     2221 cgtgcacgtg cggcaggagc tcggcagcgg tgagtacggc gatcactccc ggcacggcgc
     2281 gcgccgcctc cacgtcgatg cggcggatcc gggcgtgggc aaaggggctg cgcacgatcg
     2341 cggcgtgggc cacgttggcc ggcgcgaggt catcgacgta gcgaccgttt ccggtgatca
     2401 gccgacggtc ctcgacccgc cggaccgacg taccgctgta gcgctgggtt tgcacaaccg
     2461 tctccctagc ctgtgatgcc cttggcgacc gtgacgaacg agcagtggcc gcgactcacg
     2521 agacgcccac gctgttcaac gcgcgctcgg tcagggtgcg caccaggtct tcgcgatagg
     2581 ccgccggggt ggccaggtcg tcgctcgggt gcaccgactc ggcgaccacc gcggccgcct
     2641 cggccgcgct cgccccgccg gccagtgcct cctcgccggc acgggcccgc agcgccgtgc
     2701 tggcgacgtt ggtcaacccg atccgccagc catcagggcc ggcctgcacg ccgacgccga
     2761 cgatcgccca gtcagcgcga cggcggcgga acttcacgta gtcgtgcgga ccgtcgagca
     2821 ccggaaagct gacctcggtg atgagctcgt cgggctcgag cggggtggtc atgaagtcga
     2881 gcaggaagtc ggttgcggcg taacggttgc gggtggtgac gatctcggcg tcgagcatca
     2941 tcgccaccgt gcagtagtca ccggcgctgt cggcgtgtgc gagcaccccg ccgatcgtgc
     3001 cgcgggcccg cacctgggcg tccccgacct cgccagcgac ctgggccagc agcggcaggg
     3061 cgctcgcgac gacggggctg cgggcgatgt cgacgtgccg ggtaagcgcc ccgaggcgca
     3121 gccgcccatc ctcgaccttg atgtagcgca gctcgtcgag gccagcgagg tcgacgagca
     3181 gcaccggctc ggccagccgc agcttcatca gcgggatcag gctcatgccg ccggcgatga
     3241 ccttggcatc ctcgccgccg gcccggacag cctcggccgc ggcggcgagg tcggtggccc
     3301 ggacgtagtc gaacgtgctg gggatcatgt cagcgaccca tctggtaggt cgcggcagcg
     3361 gcaaggaccg ccttctcgat gttgacgtag ccggtgcagc ggcagatgtt gccctccagc
     3421 ccgaccccga tctcgtccgc ggtcgggtcg gggttgctgg cgagcaagga caccgtcgac
     3481 atcatgaaac ccggggtgca gtagccgcac tgcagcgcgt gctcctcgcg gaatgcctcc
     3541 tgcaccgggt gcagtccttc ggcggtggcc aggccctcga cggtggtgac cgaccggccg
     3601 tcggcctgca ccgcgaagat cgtgcatgac ttgacggcct caccgtcgag cagcaccgtg
     3661 caggcgccgc actggctggt gtcgcacccg acgtgggttc ccgtcagcgc gagccggtca
     3721 cgcaggtggt ggaccagcag cgtcctgggc tccacgacgt cggtgcgggg ccggccgttc
     3781 acctcgatcg tgacgacgtg ggttgggagc tcgggcgacg acacctagtt gcctcctcgg
     3841 cgttggtgcg gacaggaatg cgcgccggtg gaccgcacgg tcacgccttg cgctcgtgac
     3901 gcatggtcat ccgggcaacg gcgatcgcga cgagcaggat caggccgttg aacacgggct
     3961 cgacgtaggt ctgcgcgccg agcatgaaca tgccggtggt tcccgtggcc acgaggtaaa
     4021 cggccaagaa tgtgccccag acgttgtagt ggccgggccg gatcgctgtc gcgcccagga
     4081 aggcggcggt gaaggcgggc agcaggaacg acgtcatcga gtcggcgctg ctggagccga
     4141 cccgggcgga ctggacgatg ccgcccaggc cacagagcag ggccgacatg acgaacgtca
     4201 tgaggacgag gaatggcacc cggatgccag ccagtcgcgc ggcggtctcg ctgcccccga
     4261 cggcgtacat gcgccggccg gtggggcggt agctgaggaa gtaccagatc agcacgccgg
     4321 ccaccagcgc gtagataaac gcgagcggta cgccgaacgg gcgggcctgg ccgaggtgct
     4381 tgaacgacgg cgggatgttg gccgagatca gctggccgcc ggagtagagc aacgagaggc
     4441 cgtagagcgc ggagcccacg cccaacgtca ccacgaggga gttgagtccc gcgtaggcga
     4501 cgagcaaccc gttgacgacg ccgatgacca ggctgaccag cagtcccaac gcgatcgcct
     4561 gccacaccgg ccagtcgttg cgcgagagca ccccgacggt gagcaggctg gcgatcgtgg
     4621 cggtgtagcc caccgacagg tcgaactgct gcacgatcaa cggcatcatg atgcccagcg
     4681 agatgagcgt gagtgcagcg ctgccggtga ggatcgacgc gaggttctgc gtcgtcgcga
     4741 agctgctggg ccgcagcagg ctgaagacca cgacggccag caacagcgcc agcagcaggc
     4801 cataacgctg cagcaccatc accatccggc ccggcccgcc gctggtgcgg gcagcggtgg
     4861 cgaccgaggg tgccaccgag tccgtcgtga cgtcggtgcg ctccgccgcg tcggcgttgg
     4921 tcatcgtgct gcctcctgct cggccctacg catcacttca cgcctccggc ggccgtggca
     4981 cctgtgccct gcgcgctcga ccggtcatag acggccgcca gcagccccgc cttggagacg
     5041 tcactcggcg cgagctcggt gctgatccgg ccatcacgca tgacgagcac gcggtcggcg
     5101 tacttgggca cctcttcgat gtccgactcg agccagagca cggccaggcc gtcgtcctga
     5161 gcccgttgcc ggatcgcccg catgatctcg gccttggcgc ccacatcgat gccctgcaag
     5221 ggctcgtcga gcaccaggag cacgaggttc ttgcgggtcc acttgcccag cgacaccttt
     5281 tgctggttgc ccccggagag cgtgttcacg accgcaccgg gttcgggcgg agccaccgcg
     5341 agctcctgga tgacccgctt ggccatcgcc tgaaggcggt gcacggagac gcccacggca
     5401 tgcgtgagtc cgtcgtacga cgtgaccgcg agattttcca tgatgggcag cccgaccgcc
     5461 aggccgtcgc cgcgctcctg cggcgagtag cccatgccgg cggcggaggc gtgccgcggc
     5521 gtgagcctgg tcatcgggtg cccgtgcagc cggacctcgc ccgcagtgag cgcctcgatg
     5581 ccatagacga gccggcccag ctcggagcgg cccgaaccga ccaggccggt gactgcgacc
     5641 acctcgcccg ggtggatggc catgctgacg tcgtgaactc gccggccgct gatgccgcgc
     5701 agctccagca ccggctgtcc cgtgcgggcc gaggtctcat cgcggttgat cgccggggcc
     5761 tcgctgacgg tgatgcggcc gatgatgtac ctgctgaccg cggcctcatc ggtctcgctc
     5821 accggtccgg tgaacacctg ggcgccgtca cgaaggatgg tcacctcatc ggctgcggca
     5881 aacacctcgt cgagccggtg ggtgacatag acgatggcga ccccttggct cttgagcgtg
     5941 cccaggatgg cgagcagccg atcgacctcg gagcgcggca gccgggcggt cggctcgtcg
     6001 aggacgagca cgctgtgcgc atagtcgagg ccctgcaggg cccgtgccac cgcgaccatg
     6061 gtctgctcga ccgggcccag ggtagccacg gtgcgacgtg ggtcgaggtg caggcccagc
     6121 cggcccagct cggctgcggc cgagacgatc tgacgacgcc accgaatggt gccggtgccg
     6181 tcggtcttga acggtacgtc gagagtgagg ttctcggcga ccgacaggcc ggccaccagc
     6241 cccaggtcct ggtgcacgaa cctgaggccg gcggcgtgag cctgccgggg attgtggtgg
     6301 gggggcagct cggtgccgtc gacaacgacc tgccccccgt ccgcctggtg gaagccggac
     6361 agcaccttga tcagcgtcga cttgcccgac ccgttgcggc ccagaagggc gtgcaccgag
     6421 gccttgcgga catggaagtc gaccgatcgc agcgcctggg tggcgccgaa gtgcttgctc
     6481 acgtcgctgg tctgcagtgc cagcaccggc tccacagtca ccgagtcatt cggcatcaga
     6541 gaccctcaca gctccttcag cgcaccgggc aaccgactac ttgacgcccc acagctcctt
     6601 gtacttggcg acgtagtcga tgccaccggc ccagccactc ttgagctcct catcgctgag
     6661 ctcgccaagg ttgctcttat cgatgatccg gaccgggatc ttctggtcca cgacctcctg
     6721 cccgttgagc agtcggttga ggttgtccag tgctgcccag gcctcccact cgtaggggtt
     6781 gccgatggtc atggtctgga actggtcgtc ccggatcagc tgcaggttct gggcgttggc
     6841 gttcatgccg atgcccttgg cctcggtgct gatgccgctg gccagcacct gcgtcgccgc
     6901 gcgcgccatg cacgcgtcga agcaccagac ccagttgatg gacgggtcct gctgcagcgc
     6961 cgccacggcg tggttgccgg gctgggtctc caggttagcg atctggaatt ccttgacagt
     7021 gaccttgcac tcggggcact tggtcttgtc ctcgagcacg gacatgatgc ccttgtactc
     7081 gccgttcttg acgacctcga actcgttgtc gagcaggacg agggcgttca ccttgccctc
     7141 gctcttggcg atcatgtaat aggccgcgag ctctccggcg tacacccagt cgtgtgacac
     7201 gtcagggatg ctggccggct cgttgctgag gttggccacg ctgaccatgg ggatgccagc
     7261 cttgatcgcg cgctggatgc cctcttgggt gacccgggtg tcgaccaggt cgaggatgat
     7321 cgcgtcaaac tttgcgtcga tcgccgcgtt gatcgcctgc agctgcttgg ccgggttgcc
     7381 ctgaccgtcg aaaagcgtgg tcttccagcc gatcagcttg gccgcgacct cctgggcgcg
     7441 agccggtcgg gcacagcctt ctgcgttctg gtcacaggtg atcagggcaa cccgcatgcc
     7501 gggcttgggg gctggcgcct cggtcggccc ctgccactcg ctgttggcgg ccgaccactc
     7561 gtcgacgatc ttcttggcat tctcgaccgc ggccgacgag tcgccgccag cggccgccga
     7621 agagccgctg ctcggcgcct tggtgtcggc tgccgtaccg ctgtcaccgc cggacgaaca
     7681 cccggctgcc gccatcgcca ggacgacagc agcaccaacg atgctcagcc atgcccgacg
     7741 ccgccgagcc ggacgcggtc ggccgctctc cagatcttga tccttgcctg ccgccatcac
     7801 gcttctcctt tttgacgcgc ggacatgtgc ctgcgagcag atcgaatggg ccgaagatat
     7861 attagtactc cggaggggtc aatagcctgc tgttgaccga ttcggaaacg attctgagat
     7921 ctgcgaagac tgccccgtga gggacgagcc aacccgggtc gcccggcaga ggcggccgcc
     7981 tgccgatctg cggtctagcg gccggtgaag tgtgccgggc gatgggcaag aaacgcggcc
     8041 agcgcttcgc gatggtctgc ggtcgcccaa gcgcacttcc acgccaggcg ttcggcgcgc
     8101 agggcgccct cgtccgccgg ctgcgccacc agcgacttcg ccaaacgggc ggccaacggg
     8161 ccggccttgc gcagcgagtc ggccagcgtc agggcagccc cgcgggggtc ggtggccacc
     8221 tccgtgacca ggccgatctc gcgacccagc tgggccgaga tcggctcccc ggtcatgacc
     8281 attcgggtcg cccacgaggt gccgaccagc cgcggcagcc gtgccgtgcc ccccatgcct
     8341 ggcagcaggc ccaggcgcac ctccgggaac cccagccggg cctcgggtgt cgcgatgcgg
     8401 acgtccgtgc agagcgcgat ctcgagcccg ccccccaggc agtgcccacg gatcgccgcg
     8461 atggtcaggc agggcagcgt ctcgagcgac cgggtccagt gcgccgcgag ctcgacacca
     8521 ccccacgggt cggggtctgt ggtggcggcg agctccttga cgtcggcgcc gacgctaaac
     8581 acgtcatcag caccgcggac caccacggcg agcagggtgt cctcgtcgtg cagcgggcgc
     8641 agcgcggcgg cgagctcgcg ctggcactgg tcgtcgagga cgttgagcgg cggccggtcg
     8701 atgaccagct cgacgacgcc ggggcagggt cgctcgaccc gtacgacgcc gctggggctc
     8761 accgtgttcc gagcacggtg ggggcgaggt gctcggcgat ggcgaaggag gaggtggctg
     8821 cgggtgaggg tgcgttgcgc acggagagga tgccgtcgac gtcgaccagg gcgaagtcgt
     8881 cgaccagtcc gccggtgatg gtgacggctt gggcgcggac cccggcgcgg gctcgttcca
     8941 cgtcgttctc gccgaggtcg ggcacgtagc ggcgggccgc ggcgacgaag gctttgcggc
     9001 tggcggccac ggccagctcg cgggccccgg ccttccagtg cttttgggcc agcttgtaga
     9061 agccgggcca gcgcagggtg gcggccacgt ctttggggtc gacgcgggtg gcgcggtagc
     9121 cctcgcggga gagggcgagc agcgcgttgg gtccgacgag gacctcgtcg tggacggtgc
     9181 gggtcaggtg cacgcccagg aacgggtagc gcgggtcggg caccgggtag atcagcgagc
     9241 ggaccaggtg ggtccgggtg ggcagcagcc ggtagtacat gccgaggaac ggcacgatcg
     9301 ccgggtcggc cggcccgccg ccgaggcggc tgacccggtc agactgcaac ccggcgcaga
     9361 ccaccagccg gtcgaaggtg tggctcgccc cgccggcgag cacctggatg cgcccgttgc
     9421 gccgggtgat cgcggtcacc gggctgtcgg tgtgcaggcc ggcgccggcc ttgacgacct
     9481 cctcggccac cgtcgcggcc accgccggga agtcgaccac cccggtgtgc ggggagtgca
     9541 ccgcggcgta gccgcgcacg tgcggctcga tctcggccat cgcggcgccg tcgagccagg
     9601 ccaggtcggg caccccgttg gcggtggccc ggcggtggat ctcctccagg ccggcccgtt
     9661 cggactcgtc gagggcaacc acgagcttgc ccgactcctt gaacggcagg ttgttggcct
     9721 ggcagtactc ccgcagcagc ccccgcccgc gggtgcacag ctgggccttg agcgagcccg
     9781 gcttgtagta gagcccggcg tggaccaccc cgctgttgtg gctggtctga tgctgaccga
     9841 tcgccgattc cttctccagg atcgtgaccc ggcacgaagg atcagacaga cccagctgac
     9901 gagccagcgt caacccgacg atgccggccc cgacgattcc gacgtccacg gctcacccca
     9961 ctcgcatggc aacggtcttg acccgggtgt agaacccgag cccttccaca ccctgctcct
    10021 tgaacaagga gcccgagtcc ttgaacccac caaacggcat gtgcacgtcc cagcccgagg
    10081 tgggcaggtt gaccgccacc tgaccggtcg cgacccggtc ggcgaactgg tgcgcggccg
    10141 acaggtcacg ggtgaagatg cccgcggaca gaccgtagtc ggagtcgttg accaggtcga
    10201 tcgccgtgtc gaggtcggtc accgtggtcg ccgcgagcac cgggccgaag acctcctcgc
    10261 gccacagcgc ggtgtcgggc ttgacctcga tcag
//
LOCUS       DATIBB010000150         2247 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_1934590, whole genome shotgun sequence.
ACCESSION   DATIBB010000150 DATIBB010000000
VERSION     DATIBB010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2247
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1934590"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            188..1666
                     /locus_tag="VLC50_05795"
     CDS             188..1666
                     /locus_tag="VLC50_05795"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HSF27013.1"
                     /translation="MQELFEAVATLATTRTSSKVLDEMVRLAGTLTHAGSVTLSLPDS
                     DKGQRDIIAWHSKRASRARSKDRPPPERIVLPVELAHHVAGLLEVSDPADGSFGEHDE
                     LAARVLVAAAALALDNARAFEQGAHHRRRLEAMAEVQDALLTTFDSRQVLSLVADRAR
                     DVVGADVAAIVVPLTETDLIVDVVAGTAGEQALGRRIARAGSLCGEVMATGRARVVDP
                     SKDACVFATVWPWQQHAGAILVPLRTAAGVAGVLLVINTVDRQASFDLNQAATLGAFA
                     GQAALAIERTQARDDRLRLSLYEDRERIARDLHDHVIQRLFATGLTLQRFGGRHDTGP
                     LDDMLQTVVDEIDTTIAQIREVIHQLHEHPDEATFVASLRRVISDAEGVLGFAPALEP
                     GARPDQVPQHLRADVLAVLREALSNAARHAHAHAVHVALDVGDRLRLAVDDDGDGLGE
                     SSRRSGLLNLRRRAEAHGGHLRIDTGATGRGTRLVWDVPTRP"
     gene            complement(1779..>2247)
                     /locus_tag="VLC50_05800"
     CDS             complement(1779..>2247)
                     /locus_tag="VLC50_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HSF27014.1"
                     /translation="GRGVDIAKMVHDELPGTRAMMLTSYDDDETLFDAIAAGVTGLLL
                     KQVRGTDFVDAVRRVAAGESMLHPAVTKRLMDRVGGRRAEPDDDGLASLTPQERRVLG
                     CIGQGMANREIAEVLGVSEKTVKNHVTKLLGKLGLQRRTQAAVLASRLDAPAD"
BASE COUNT          302 a          856 c          793 g          296 t
ORIGIN      
        1 tactccctcc acggcagcca tcacgcgcac cggtgatcag catcagctcg cggccgcccg
       61 acgtcgctgc tcccgcttga tctcaccgac ttcaacgccg gcgttgctgc ccccggcagg
      121 tgctccctcg ccgacgcgag gcgcgtcatg cgcgccacgt cgtaggacgg ctgccagact
      181 cgcccccatg caggagctct tcgaggcggt cgccaccctg gcgaccaccc ggacgagctc
      241 gaaggtgctc gacgagatgg tccgcctcgc gggcacgctg acccacgccg gcagcgtcac
      301 cctcagcctg cccgacagcg acaagggtca gcgcgacatc atcgcgtggc actccaagcg
      361 ggcctcccgg gcgcggtcga aggaccgccc gccgccggag cgcatcgtgc tgccggtcga
      421 gctggcccac cacgtggccg gtctgctcga ggtctccgac ccggccgacg gcagcttcgg
      481 cgagcatgac gagctggcgg cccgggtgct ggtggccgcc gccgcgctgg ccctggacaa
      541 cgcacgggcc tttgagcagg gcgcgcatca ccggcgccgg ctcgaggcga tggccgaggt
      601 gcaagacgcg ctgctcacga cgttcgacag ccggcaggtg ctctccctgg tcgccgaccg
      661 ggcccgcgac gtggtcggcg ccgacgtggc cgcgatcgtc gtaccgctga ccgagaccga
      721 tctgatcgtc gacgttgtcg ccgggacggc cggcgagcag gcgctcgggc ggcggatcgc
      781 ccgggccggc tcgctgtgcg gggaggtcat ggccaccgga cgggcccggg tcgtggaccc
      841 gtccaaggac gcgtgcgtgt tcgcgacggt ctggccctgg cagcagcacg cgggcgccat
      901 cctcgtgccg ttgcgtacgg cggccggcgt ggccggcgta ctgctggtca tcaacaccgt
      961 cgaccggcag gcaagcttcg acctcaacca ggccgccacg ctgggcgcgt tcgccgggca
     1021 ggcggcgctg gcgatcgaac gcacccaggc ccgcgacgac cggctgcggc tctcgctcta
     1081 tgaggaccgc gaacgcatcg cccgcgacct gcacgaccac gtgatccagc ggctgttcgc
     1141 caccgggctg accctgcagc gcttcggcgg ccgccacgac accggtccgc tcgacgacat
     1201 gctgcagacc gtcgtcgacg agatcgacac gaccatcgcg cagatccgcg aggtgatcca
     1261 ccagctgcac gagcatcccg acgaggccac gttcgtcgcg tcgctgcgcc gggtcatcag
     1321 cgacgccgag ggggtgctgg ggttcgcgcc ggccctcgag ccgggtgcgc gccccgacca
     1381 ggtgccgcag cacctgcggg ccgacgtgct ggcggtgctg cgcgaggcgt tgtccaacgc
     1441 cgcccggcac gcgcacgccc acgcggtcca cgtcgcgctc gacgtcggcg accggctgcg
     1501 gctggccgtc gacgacgacg gcgacgggct cggggagtcc tcgcgccgca gcgggctgct
     1561 caacctgcgg cggcgagccg aggctcacgg cggtcacctg cgcatcgaca ccggggcgac
     1621 cgggcgcggc acccggctcg tctgggacgt gcccacccgc ccctgacacg tccgtgcccg
     1681 cccggaagcg ccgatccggc cagatcgtgc gactccgtgg tcgtcagagc gccggaaagt
     1741 cgcacgcagc gccgaaaaca cgcacgcgcg gcggccggtc agtcggcggg cgcgtcgagg
     1801 cgggaggcga gcacggcggc ctgggtgcgc cgctgcaggc ccagcttgcc gagcagcttg
     1861 gtgacgtggt tcttcacggt cttctccgag accccgagca cctcggcgat ctcccggttg
     1921 gccatgccct gcccgatgca gccgagcacc cggcgctcct gcggcgtcag ggaggccagc
     1981 ccgtcgtcgt ccggctcggc ccggcgacca ccgacccggt ccatgagccg cttggtcacc
     2041 gccgggtgca gcatcgactc gccggccgcg acccggcgta ccgcgtcgac gaagtcggtg
     2101 ccgcgcacct gcttgagcag caggccggtc acccctgccg cgatcgcgtc gaagagcgtc
     2161 tcgtcatcgt cgtacgacgt cagcatcatg gcccgggtgc cgggcagctc gtcgtgcacc
     2221 atcttggcga tgtcgacgcc gcgcccg
//
LOCUS       DATIBB010000151         2380 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_1673849, whole genome shotgun sequence.
ACCESSION   DATIBB010000151 DATIBB010000000
VERSION     DATIBB010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2380)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2380)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2380
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1673849"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..396
                     /locus_tag="VLC50_05805"
     CDS             <1..396
                     /locus_tag="VLC50_05805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF27015.1"
                     /translation="RLADIEGYNDWMPTKGSIRRHSQQTSPGESALGTSYVDQTRFGP
                     TPGEIVEFQPPHRLVYHWWDRSKTGRLKTEGWPGYVLVAADGSTTLVRHHARMRTYGV
                     YRLAGPVLRRIALRERTTTIDALGASFAG"
     gene            462..761
                     /locus_tag="VLC50_05810"
     CDS             462..761
                     /locus_tag="VLC50_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018729466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF27016.1"
                     /translation="MESVYPGDRDREPDAMPGVYPDDELGDDVEPLILHVDGEVFALR
                     PDTHGGMGYTWLSGPNPGYGFGMGPARDLSLDEHCEHIRGFLAMIDPQTGYIADD"
     gene            complement(862..1356)
                     /locus_tag="VLC50_05815"
     CDS             complement(862..1356)
                     /locus_tag="VLC50_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009771689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HSF27017.1"
                     /translation="MKDAVDRVLEQWHAERPDIDPSAMGVIGRLSRITRLLERSLAEV
                     FAQHGLQPGEFDLLATLRRSGAPYELTAGALAATSMVTSGAITYRIDRLVAKGLVTRE
                     VDPDNRRSVLIGLTDAGRELVDTAVVDHYRNENRLLGPLTSKERTQLAGLLRKLLVEH
                     ERPD"
     gene            1453..>2380
                     /locus_tag="VLC50_05820"
     CDS             1453..>2380
                     /locus_tag="VLC50_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019984820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HSF27018.1"
                     /translation="MALSRTTLSVTALTAVTPMVWGTTYVVTTELLPPDRPLLAGMLR
                     ALPVGLLVVAATRVLPRGHWWWRAATLGTLNIGAFFALLFVSAYRLPGGVAATMGAAQ
                     PLVVAALAYVLLAERPTRWRLGWGVVGVLGVGLMVLTAQARLDGLGVVAGLAGTASMA
                     AGIVLTKRWGRPDGVGVLAFTGWQLAAGGLVLLPLALAVEGAPPALGIGAVLGYAWLA
                     LVGTLLAYVLWFRGLARLPIGAFSFLPLLSPVMATVLGWALLHQALTPLQGLGFVLAL
                     GAIAAAQASPPRLRDIRRVSARSGGRLIPVRRS"
BASE COUNT          299 a          819 c          866 g          396 t
ORIGIN      
        1 cggctcgccg acattgaggg ctacaacgac tggatgccca caaagggcag catccggcgg
       61 cattcgcagc agacctcgcc cggagagtcg gccctgggga cgtcgtacgt cgatcagacc
      121 cgcttcggcc cgacgccggg agagatcgtc gagttccagc cgccgcatcg gctggtctat
      181 cactggtggg atcggtcgaa gacgggaagg ctcaagactg aaggttggcc cggctatgtg
      241 ctggtcgccg ccgacgggag cacgacgctc gtgcgccacc acgcgcggat gcgcacctac
      301 ggcgtctatc ggctggcagg tccggtcctg cgccggatcg ccctccggga acgcaccacg
      361 acgatcgacg ccctcggcgc ctcgttcgcg ggctaaggaa tgcgtagcgt cccccggaag
      421 ggaagttgtc gtcgccgcac tcgggtcgtg atgatggcga cgtggagtcg gtctacccgg
      481 gcgacaggga tcgcgagccg gacgccatgc cgggcgtcta tcccgacgat gagctcggcg
      541 acgacgttga gccgctcatc ctgcacgtcg acggcgaggt gttcgccctg cgaccggaca
      601 cgcacggcgg catgggttac acgtggctca gcgggccgaa tcccggttac gggttcggca
      661 tgggtcccgc gcgggatctc tcgctggatg agcactgcga gcacatccgc ggcttcctcg
      721 cgatgatcga cccccagacc ggctacatcg cggacgactg agccacgacc accgtccttg
      781 gcaaggcgtt gtggcactag gcaaggcgtg cacgcctcgc ctagtgagcg aacgcccact
      841 tggcgtggtc aatcgcaggg gtcagtcggg gcgctcgtgc tcgacgagca gcttgcgcag
      901 cagcccagcc agctgggtcc gctcctttga cgttagcggg ccgagcaggc ggttctcgtt
      961 gcggtagtgg tcgacgaccg cggtgtccac cagctcgcgg cctgcgtccg tcaggccgat
     1021 gaggacgctg cgccggttgt ccgggtcgac ctcgcgcgtc accaggccct tggccacgag
     1081 ccggtcgatc cgataggtga tcgcccccga ggtcaccatc gaggtggcgg ccagcgcccc
     1141 ggcggtcagc tcgtacggcg cacccgaccg gcgcagcgtg gccagcaggt cgaactcgcc
     1201 cggctgcagc ccgtgctggg cgaagacctc ggcgagcgag cgctcgagca gccgggtgat
     1261 gcgcgagagc cgcccgatga cgcccatcgc agacgggtcg atatcggggc gttcggcgtg
     1321 ccactgctcg agcacccggt caacggcgtc cttcatgccg acctctcagc gctgaactgt
     1381 ttgacagcac gctatcaagc gcctagcgtc cttcagtgtt gaacagttca ggactgaagg
     1441 gtgggtgagg tcatggcact ctcgcgtacg acgttgtccg tgaccgcgct gacggcggtg
     1501 accccgatgg tctggggcac gacgtacgtc gtcacgaccg agctcctccc gcccgaccgt
     1561 ccgctgctgg ccggcatgct gcgggccctg ccggtcgggc tgctggtggt ggcggccacc
     1621 cgcgtgctgc cacgcgggca ctggtggtgg cgggccgcca ccctcggcac cctcaacatc
     1681 ggcgcgttct tcgccctgct gttcgtctcg gcctaccggc tgcccggcgg cgtcgcggcc
     1741 acgatgggcg ccgcgcagcc gctggtggtg gccgcgctgg cctacgtgct gctcgccgag
     1801 cgcccgaccc ggtggcggct gggctgggga gtcgtcgggg tgctcggggt cgggctcatg
     1861 gtgctgaccg cgcaggcccg gctcgacggc ctcggggtgg tggccggtct ggcgggtacg
     1921 gcgtcgatgg ccgccggcat cgtgctgacc aagcggtggg gccgtcccga cggggtgggg
     1981 gtgctcgcgt tcaccggctg gcagctcgcg gccggcgggc tggtgctgct cccgctggcg
     2041 ctcgcggtcg agggggcgcc cccagcgctc ggcatcggcg cggtgctggg ctatgcctgg
     2101 ctcgccctcg tgggcaccct gctcgcctac gtgctgtggt tccggggact ggctcggctg
     2161 ccgatcgggg cgttctcgtt cctgccgctg ctgtccccgg tgatggccac ggtcctgggc
     2221 tgggcgctgc tgcaccaggc gctgacgccg ttgcagggcc tcgggttcgt cctcgcgctc
     2281 ggggccatcg ccgcggcgca ggccagccct ccccggctgc gcgacatccg ccgcgtctcc
     2341 gcgaggtcgg gtggccgttt gattcctgta cgtcgctcct
//
LOCUS       DATIBB010000152         5194 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_753025, whole genome shotgun sequence.
ACCESSION   DATIBB010000152 DATIBB010000000
VERSION     DATIBB010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 5194)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5194)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5194
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_753025"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1794
                     /locus_tag="VLC50_05825"
     CDS             <1..1794
                     /locus_tag="VLC50_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012921347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HSF27019.1"
                     /translation="GHRVFRRLGDADVPTFAFTNGLAIGGGVEIGLHCNYRTVISSLP
                     ALGLSECYLGLVPGWGGAWLLPNLVGAEAAVTVMVENPLNSNTLLRAPEVMALGLADA
                     VFDGADFLAQSLRWAATVVTGETVVARAEPDRGAAWDAALARGRAIADVKTGGAAPAP
                     YRALALIEAARTSSRDEGFAAEDEALADLLLTDEMRAGVYTLDLVTKRAKRPAGAPRP
                     TLARPVTKVGIVGAGLMASQIALLLARRLQVPVVLTDVDQARVDHGVAAVHAEIATLA
                     RKGRIRPDAANRLTALVTGSVSHDGFTDADLVIEAVFERLDVKKAVFAELESVVRPDC
                     VLATNTSSLSVSAMAAGLAHPERVVGLHFFNPVAVMPFLEVVRAEATDDASLATAFAV
                     AKELKKTAVLVKDSPAFVVNRLLGRWMGEVSRIVDEGTPLPVADGAFAGLAPMPPFML
                     LGLVGPAVALHTAVTLHDAFPERFYVSANLRRVVDAGKTAIYTFVDGKPVVDPEVAAL
                     FETPAEPVELTREQVRHRVLAALAEEAQIMLDDGVVAEPADIDLGLITGAGFSPWNGG
                     LTPLLDRTGIAEQVTGRRFLPEGVASVACEH"
     gene            1860..2657
                     /locus_tag="VLC50_05830"
     CDS             1860..2657
                     /locus_tag="VLC50_05830"
                     /EC_number="1.1.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007075772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003858 - 3-hydroxybutyrate dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate dehydrogenase"
                     /protein_id="HSF27020.1"
                     /translation="MPIASDPTTSRTTDAAGQLDLRGRRALVTGAGSGIGLACARRLS
                     TAGAQVCVVDIDADAATEAAGLIGGEAVVCDLADLDAVDRLDLDVDVIVNNAGIQHVA
                     PVHEFPPQAFHRILAIMLEAPFRLVRGALPHMYEQGWGRIVNISSVHGLRASPYKAAY
                     VAAKHGLEGLSKVIALEGAGRGVTSNCVNPAFVRTPLVDKQIAAQADARGIEPDEVIG
                     GVMLAPVAIKRLIEPDEVAELVAFLCSPGASFVTGASYTIDGGWTAH"
     gene            2681..4582
                     /locus_tag="VLC50_05835"
     CDS             2681..4582
                     /locus_tag="VLC50_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020518876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HSF27021.1"
                     /translation="MAAMAALSFLELLDQEASAVEFERPLVEARARGADADELADLAR
                     AKLLALKVRGVLEDRRRRESELTALFETARDLTALRDLDSVLQAIVRRARLLLGSDVS
                     YLSLNDEERGGTYMRVTEKIVSVAFRNVELPMGAGLGGLVAQTATPYASDDYFTDERF
                     HHTSVIDEAVREEGIVSIVGVPLALGSQVVGVLYAANRSRRPFSRADVNLLSSLAAHA
                     VVAIDNARLLGETREALDELNATSNLLRAHSASVERAADAHDRFAELVLRGGGVEEVA
                     AALTDILGGSLRVIGADGALLVGKADGAAASNPAVDDPALVAALASARQSGRAVVAGR
                     WTVAAGLAGTEYLGALVLEHVDPPEQADLRIFERAAVVMALLLLFRRSVAEVESRVRG
                     ELLVDLLREPLLNPAGLDDRARLLGVDLNRPHCMAVTAMRDAARERASTAASHLASSR
                     SGLAAAYQGRLVLLLPTGDPAECGQALATALARAGLPEHTVGVAGPIGSPAEVAPAFT
                     ESCRCHDALLALGQTGQVGGADELGFLGQLLGRTPDLGGFVGRVLGPVLDYDRRRGTD
                     LIATLQAWFACGGHLGPTGAALHIHVNTVGQRLERITRLLGDGWQEPDQALELHLALR
                     LHQALGAAG"
     gene            complement(4485..>5194)
                     /locus_tag="VLC50_05840"
     CDS             complement(4485..>5194)
                     /locus_tag="VLC50_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008359055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HSF27022.1"
                     /translation="YFAMVTLAFAQMVYFVANQWRGLTGGENGLQGVPRSLFGVDAVE
                     TDAFGYYFAVLPLLVLGCWAAWRIVHSPFGRVLVAVRDNPDRARALGLPIDRYKLVAF
                     VLSAGLAGIAGGVFAIGHGFASLQEVAWTTSGKVVLIMVLGGIGTLWGPMIGAALVVL
                     LEDWLATSGFEGTGLVTGLIFVVVVLAFRKGLWGTATTLWAGLLSRRRRAPGAGAAPD
                     AAPALDPAPASHRPADG"
BASE COUNT          711 a         1944 c         1828 g          711 t
ORIGIN      
        1 ggtcaccggg tcttccgtcg gctgggcgac gccgacgtgc cgacgttcgc gttcaccaac
       61 gggctggcca tcggcggcgg ggtcgagatc ggcctgcact gcaactaccg cacggtgatc
      121 tcctcgttgc cggctctggg actgtccgag tgctacctcg ggctggtgcc cggctggggc
      181 ggtgcgtggc tgctgcccaa cctggtcggc gccgaggccg cggtgaccgt gatggtcgag
      241 aacccgttga acagcaacac gctcctgcgc gcccccgagg tgatggcgct cgggctggcc
      301 gatgcggtct tcgacggcgc cgacttcctc gcccagtcgc tgcgctgggc ggccacggtc
      361 gtcaccggcg agaccgtcgt cgcgcgtgcc gaacccgatc gcggcgccgc ctgggacgcc
      421 gccctggccc gcggtcgggc gatcgccgac gtcaagaccg gcggcgccgc ccccgcgccc
      481 taccgcgcgc tggcgctcat cgaggcggcc cgcacgagca gccgtgacga gggcttcgcc
      541 gccgaggacg aggcgctggc cgacctactc ctgaccgatg agatgcgggc cggggtctac
      601 accctcgacc tggtgaccaa gcgggccaag cgtccggccg gggcacctcg gccgacgctc
      661 gcccgtcccg tcaccaaggt cggcatcgtc ggcgccggcc tgatggccag ccagatcgcc
      721 ctgctgctgg cccggcgcct gcaggtgccg gtggtgctca ccgacgtcga ccaggctcgg
      781 gtcgaccatg gggtggcggc cgtgcacgcc gagatcgcca cgctggcgcg caaggggcgc
      841 atccggcccg acgcggccaa ccgcctgacc gcgctggtga ccggctccgt ctcccacgac
      901 gggttcaccg acgccgacct ggtcatcgag gcggtcttcg agcgcctcga cgtcaagaag
      961 gcggtgttcg cggagctgga gtcggtggtc cggcccgact gcgtgctggc gaccaacacc
     1021 tcgtcgctgt cggtcagcgc gatggcggcc gggctggcgc accccgagcg cgtcgtcggg
     1081 ctgcacttct tcaacccggt ggcggtgatg ccgttcctcg aggtggtccg cgccgaggcg
     1141 accgacgacg ccagcctcgc caccgcgttc gcggtcgcca aggagctgaa gaagaccgcc
     1201 gtactcgtca aggactcccc cgcgttcgtc gtcaaccggc tgctcggccg ctggatgggc
     1261 gaggtcagcc ggatcgtcga cgagggcacg ccgctgccgg tggcagacgg cgccttcgcc
     1321 gggctggctc cgatgccgcc gttcatgctg ctcgggctgg tcgggccggc cgtcgccctg
     1381 cacaccgccg tcacgctgca cgacgcgttc cccgagcgct tctacgtctc cgccaacctg
     1441 cgccgggtgg tcgatgccgg caagacggcg atctacacgt ttgtcgacgg caagcccgtc
     1501 gtcgacccgg aggtcgcggc cctgttcgag actccggccg agccggtcga gctcacccgt
     1561 gagcaggtgc gccaccgggt gctcgccgcg ctggccgagg aggcgcagat catgctcgac
     1621 gacggggtgg tcgccgagcc cgccgacatc gacctgggac tcatcaccgg tgccgggttc
     1681 tccccctgga acggcgggct cactcccctg ctcgaccgca ccggcatcgc cgagcaggtg
     1741 accggacggc ggttccttcc cgagggggtc gccagtgtcg cgtgcgaaca ctgatctccc
     1801 agcagctatg tgggcgccga ccctggggcg acggcacggc cgggccttac cgtcgctgcg
     1861 tgcccatcgc atccgacccc acgacctcac gcacgactga cgcggcgggc cagctcgacc
     1921 tgcgcgggag gcgggcgctg gtcacgggcg caggcagcgg gatcgggctg gcctgcgcgc
     1981 gccggctgag cacggccggc gcccaggtgt gcgtcgtcga catcgacgcc gacgccgcga
     2041 ccgaggcggc aggactgatc ggcggggagg cggtggtctg cgacctggcc gatctcgacg
     2101 ccgtcgaccg gctcgacctc gacgtcgacg tcatcgtcaa caacgccggg atccagcacg
     2161 tcgcgccggt gcacgagttt cccccgcagg ccttccaccg catcttggcg atcatgctcg
     2221 aggcgccgtt ccggctggtg cgcggcgcgc tgccgcacat gtatgagcag ggttggggcc
     2281 gcatcgtcaa catctcctcc gtccacggcc tgcgcgcctc gccctacaag gccgcctacg
     2341 tggccgccaa gcacggcctg gaggggctgt ccaaggtcat cgcgctcgag ggcgcaggcc
     2401 gtggggtgac gtccaactgc gtcaacccgg cgttcgtgcg aaccccgctg gtcgacaagc
     2461 agatcgccgc acaggccgac gcccgtggca tcgagcctga cgaggtgatc ggcggggtga
     2521 tgctcgcccc ggtcgccatc aagcggctca tcgagcccga cgaggtggcc gagctcgtgg
     2581 cgttcctgtg cagccccggc gcttccttcg tcaccggcgc ctcctacacg atcgacggcg
     2641 gatggaccgc gcactgagcc gacgcccacg acagtccaca atggcggcca tggccgcgct
     2701 gtcctttctc gagctgctcg accaggaagc ctccgccgtc gagttcgagc gcccgctcgt
     2761 cgaggctcgc gcgcgcgggg ccgacgccga cgagctggcc gacctggccc gcgccaagct
     2821 gctcgcgctc aaggtgcgtg gcgtgctgga ggaccgccgt cgtcgcgagt ccgagctgac
     2881 cgcgctgttc gagaccgccc gggacctcac tgccctgcgc gacctcgact cggtgctgca
     2941 ggccatcgtg cgccgggccc ggctgctgct gggcagcgac gtgagctacc tgtcgctcaa
     3001 cgacgaggag cgcggcggca cctacatgcg ggtcaccgag aagatcgtct cggtggcctt
     3061 ccgcaacgtc gagctgccga tgggcgccgg gctcggtggg ctggtcgccc agacggcgac
     3121 cccctacgcc agcgacgact acttcaccga cgaacgcttc caccacacca gcgtcatcga
     3181 tgaggcggtg cgcgaagagg gcatcgtgtc gatcgtcggg gttcccctgg cgctgggcag
     3241 ccaggtcgtc ggcgtgctct acgcggccaa ccggagccgg cgcccgttca gccgcgcaga
     3301 cgtcaacctg ctcagctcgc tcgccgcgca cgcggtggtg gcgatcgaca acgcccgcct
     3361 gctcggggag acccgagagg ccctcgacga gctcaacgcc acgagcaacc tgctgcgggc
     3421 gcacagcgcc tcggtcgagc gggccgccga tgcccacgac cggttcgccg agctcgtgct
     3481 gcgcggcggc ggagtcgagg aggtcgccgc tgccctgacc gacatcctgg gcggctcgct
     3541 tcgagtcatc ggcgccgacg gcgcactgct ggtcggcaag gccgacggcg cggccgcgag
     3601 caacccagcc gtcgacgacc ctgcgctggt cgccgcgctc gccagcgccc ggcagagcgg
     3661 ccgcgcggtg gtggccgggc ggtggaccgt ggccgccggg ctcgccggca ccgaatacct
     3721 cggcgccctg gtcctcgagc acgtcgaccc gccggagcag gccgacctgc ggatcttcga
     3781 gcgcgccgcc gtggtgatgg cgctgctcct gctgtttcgc cggtcggtgg ccgaggtcga
     3841 gagccgggtc cgcggcgagc tgctcgtcga cctgctccgt gagccgctgc tcaaccccgc
     3901 cgggctcgac gaccgggcac ggctactggg tgtcgacctc aaccgacccc actgcatggc
     3961 cgtaaccgcc atgcgcgacg ccgcgcgtga acgggcctcc acggcggcct cgcacctggc
     4021 cagctcgcgc agtggactgg ccgcggccta ccagggccgc ctcgtgctgc tgctgccgac
     4081 cggcgacccc gccgagtgcg ggcaggcgtt ggccaccgcg ctggcccggg ccgggctgcc
     4141 cgagcacacg gtcggcgtgg ccggccccat cggctcaccg gcagaggtcg ccccggcatt
     4201 caccgagtcc tgccgctgcc acgacgcgct gctggccctg ggccagaccg gccaggtggg
     4261 cggcgccgac gagctcggct tcctcggcca gctcctgggg cgtaccccgg acctgggcgg
     4321 attcgtcggc cgcgtcctcg gcccggtcct cgactacgac cgccggcgcg gcaccgacct
     4381 catcgccacg ctgcaggcct ggttcgcctg cggcggtcac ctcggtccca ccggcgcggc
     4441 cttgcacatc cacgtcaaca ccgtaggtca gcggctggag cggatcaccc gtctgctggg
     4501 cgatggctgg caggagccgg atcaagcgct ggagctgcat ctggcgctgc gcctgcacca
     4561 ggcgctcggc gccgccggct gagcagcccc gcccacaacg tggtggcggt gccccaaagc
     4621 cccttgcgga aggccagcac caccacgacg aagatcaggc cggtgaccag gccggtcccc
     4681 tcgaacccgc tcgtggccaa ccagtcctcg agcagcacca ccagggctgc accgatcatc
     4741 gggccccaca gcgtgccgat cccgccgagc accatgatga gcacgacctt gccggaggtg
     4801 gtccaggcca cctcctgcag cgaggcgaac ccgtggccga tcgcgaagac gccaccggcg
     4861 atgccggcca gcccggccga gagcacgaag gcgacgagct tgtaacggtc gatgggcagg
     4921 cccagcgccc gggcccggtc ggggttgtcg cgcaccgcga cgagcacccg cccaaacggc
     4981 gagtgcacga tgcgccaggc ggcccagcac cccagcacga gcaacgggag tacggcgaag
     5041 tagtagccga acgcgtcggt ctcgacggcg tcgaccccga acaaggaccg gggcacgccc
     5101 tgcaggccgt tctccccccc ggtgagcccg cgccactggt tggccacgaa gtagaccatc
     5161 tgggcgaacg ccaaggtgac catggcgaag taga
//
LOCUS       DATIBB010000153         5442 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_886693, whole genome shotgun sequence.
ACCESSION   DATIBB010000153 DATIBB010000000
VERSION     DATIBB010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 5442)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5442)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5442
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_886693"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            9..563
                     /gene="pdxT"
                     /locus_tag="VLC50_05845"
     CDS             9..563
                     /gene="pdxT"
                     /locus_tag="VLC50_05845"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018382188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HSF27023.1"
                     /translation="MTALGAGAVTVRRPEELDRVDGLVLPGGESTTMVKLAAAFELLE
                     PMRKRVAEGMPTYGSCAGMILLADRIEDGTTDQETVGGLDVVVRRNAFGRQVDSFEAD
                     VTFEGLTPPGPVHAVFIRAPWVESVGERVRPLAKVEGGPADGKVVAVRGGAHDHLLAT
                     SFHPELTGDSRVHGLFLQMVRERS"
     gene            560..1312
                     /locus_tag="VLC50_05850"
     CDS             560..1312
                     /locus_tag="VLC50_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007075759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HSF27024.1"
                     /translation="MSGHSKWATTKHKKAVIDARRGKMFAKLIKNIEVAARTGGGDPA
                     GNPTLYDAIQKAKKSSVPNDNIDRAVKRGSGAEGGGADYQTIMYEGYGPSGVAILVEC
                     LTDNRNRAAAEVRTAMTRNGGSMADPGSVAYLFNRKGVVIVPKTEGMTEDDVLLAVLD
                     AGAEEVNDLGEAFEVVSEATDLVAVRTALQDAGLDYDSAEASFLPSVNVPLDEDGAKK
                     VFRLVEALEDSDDVQNVWANYDVSDEIMEAVG"
     gene            complement(1309..1887)
                     /locus_tag="VLC50_05855"
     CDS             complement(1309..1887)
                     /locus_tag="VLC50_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013284893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HSF27025.1"
                     /translation="MFERFTEDARAVVSGAREGAVARGDGWVGSEHLLLAVLEDHSAV
                     ARALGELGLTAARVRAQLPGSGRGTPSDDDALRDLGINIDEVRRRAEERFGAGALDEP
                     PAARPAPWWRRGRATESSAHSGRPRFSPEAKKALELAVRESLRLKAGAIALDHVVLGL
                     LRADGPATQVVTALGVEPAAARKAVLRLRRAA"
     gene            complement(1890..2135)
                     /locus_tag="VLC50_05860"
     CDS             complement(1890..2135)
                     /locus_tag="VLC50_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007384656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF27026.1"
                     /translation="MTTETEVAEQATSQDPAVGLRAVAALRRLTEQLEALQVSSARAQ
                     GWSWDAIGALLGVSRQAVHKKHSGHTRRAGSPRHGGN"
     gene            2307..2993
                     /gene="ruvC"
                     /locus_tag="VLC50_05865"
     CDS             2307..2993
                     /gene="ruvC"
                     /locus_tag="VLC50_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006350318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HSF27027.1"
                     /translation="MRVLGVDPGLTRCGLGVVDGAVGRPVRLVAVDVVRTPPTDDIGA
                     RLLAIERAVEAWIHEHRPDAVAVERVFSQHNVRTVMGTAQASAVAMLVAARRGLPVEL
                     HTPSEVKAAITGDGRADKAAVAAMVARVLGLSAPPRPADAADALALAICHVWRGSISG
                     RLAAAVAAAPRSRIPAGPRARGGWGSASPVRGGAWTATAAAVAMTRSTPGVRRPSPRR
                     ADPPTSGAAS"
     gene            2990..3604
                     /gene="ruvA"
                     /locus_tag="VLC50_05870"
     CDS             2990..3604
                     /gene="ruvA"
                     /locus_tag="VLC50_05870"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020573684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HSF27028.1"
                     /translation="MIAFVHGRVAALGPDTAVVEVGGVGLSVLCTAATIGSLRVGEPA
                     RLATSLVVREDSLTLYGFADEDERVVFELVQTASGVGPRLAQAMLAVHRPDDLRRAVA
                     TDDLVALTKVPGIGRKGAQRIVLELKDRLGPPHGLTAAPRRSSDDGWRAQVHAGLASL
                     GWSTREADDAVAAVEEQASTLGADGAAPDVATLLRAALRTLDRS"
     gene            3601..4692
                     /gene="ruvB"
                     /locus_tag="VLC50_05875"
     CDS             3601..4692
                     /gene="ruvB"
                     /locus_tag="VLC50_05875"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018836746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HSF27029.1"
                     /translation="MSEDPRELVHPEASSDERAIEAALRPRSLTEFVGQERVREQLGL
                     VLEAARRRGRVPDHVLLSGPPGLGKTTLAMIIAAVMQAPLRVTSGPAIQHAGDLAAVL
                     SSLTEGEVLFLDEIHRMSRPAEEMLYMAMEDFRVDVVVGKGPGATAIPLEIPPFTLVG
                     ATTRAGLLPGPLRDRFGFTGHMDFYTPAELEHVLDRSARLLGVELHPGGAQEIAGRSR
                     GTPRIANRLLRRVRDWAQVRGDGVVHRAAARAALEVYEVDELGLDRLDRSVLLALIER
                     FGGGPVGLGTLAVAVGEERETVEEVAEPFLVREGLLARTARGRVATAAGWAHLGLRPP
                     QPAATLFGSLGEADPLERGDDDESADVAR"
     gene            4956..5231
                     /gene="yajC"
                     /locus_tag="VLC50_05880"
     CDS             4956..5231
                     /gene="yajC"
                     /locus_tag="VLC50_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007458273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HSF27030.1"
                     /translation="MLLLLAVAFYFLILRPQRTRAREAAKLQSSLAPGQTVMTSSGLY
                     GRIESLEDDVVVLEAAPGLQLRWAKAAIARVVTPVADSGSDPLDTGH"
BASE COUNT          705 a         1967 c         2029 g          741 t
ORIGIN      
        1 tgcaggtcct gacggcgctg ggggccgggg cggtgaccgt acgccggccc gaagagctgg
       61 accgggtcga cgggctggtc ctgcccggcg gcgagtccac cacgatggtg aagctggctg
      121 ccgccttcga gctgctcgag ccgatgcgca agcgggtggc cgagggcatg ccgacgtacg
      181 gctcgtgcgc cgggatgatc ctgctcgccg accggatcga ggacggcacc acggaccagg
      241 agaccgtcgg cgggcttgac gtggtcgtgc gccgcaacgc gttcggccgt caggtcgact
      301 cgttcgaggc ggacgtgaca ttcgagggcc tcacgccgcc cgggccggtg cacgcggtgt
      361 tcatccgggc cccgtgggtc gagtccgtcg gggagagggt acgaccgctc gcgaaggtcg
      421 agggcgggcc ggccgacggt aaggtcgtag ccgtccgagg tggcgcgcac gaccacctgc
      481 tggcgacctc gttccacccc gagctgaccg gcgacagccg ggttcacggg ttgttcttgc
      541 agatggtgag ggagcgttca tgagcggcca ctccaagtgg gcgacgacca agcacaagaa
      601 ggccgtcatc gacgcccggc gcggcaagat gttcgccaag ctgatcaaga acatcgaggt
      661 cgctgcgcgc accggcggag gggacccggc aggcaacccc acgctgtacg acgcgatcca
      721 gaaggccaag aagtcctcgg tccccaacga caacatcgac cgtgcggtca agcgcgggtc
      781 cggcgccgag ggcggcggtg ccgactacca gacgatcatg tacgagggct acggcccgag
      841 cggcgtcgcg atcctggtcg agtgcctcac cgacaaccgc aaccgcgccg ccgccgaggt
      901 gcgcacggcc atgacccgca acggcggctc gatggccgac ccgggctcgg tggcctatct
      961 gttcaaccgc aagggcgtcg tcatcgtccc gaagacggag ggcatgaccg aggacgacgt
     1021 cctgctcgcc gtgctggacg ccggcgccga ggaggtcaac gacctcggtg aggcattcga
     1081 ggtggtgtcc gaggcgaccg acctggtggc ggtgcgcacc gccctgcagg acgcgggcct
     1141 cgactacgac tcggccgagg cgtcgttcct gccctcggtg aacgtccccc tcgacgagga
     1201 cggcgccaag aaggtgttcc ggctcgtcga ggcgctcgag gactccgacg acgtgcagaa
     1261 cgtctgggcc aactacgacg tctcggacga gatcatggag gctgtgggct aggcagcccg
     1321 gcgcagtcgg aggacggcct tccgtgcggc ggcgggctcg actccgagcg cggtgaccac
     1381 ctgtgtcgca ggcccgtcgg cccgcagcag gccgagcacg acatgatcga gggcgatcgc
     1441 gccggccttg agccgcagtg actcgcgtac tgcgagctcc agtgccttct tggcctccgg
     1501 ggagaacctc ggccggccgc tgtgcgcgga ggactcggtc gcccgtccgc gccgccacca
     1561 gggtgccggt cgcgcggcgg gaggctcgtc cagcgcgccg gcgccgaaac gctcttcggc
     1621 gcgtcggcgt acctcatcga tgttgatgcc gaggtcgcgc aacgcatcgt cgtccgacgg
     1681 tgtcccccgc cccgacccag gtagctgagc gcggacccgc gcggcggtga ggccgagctc
     1741 tccgagcgcc cgagcgacgg cggagtggtc ctcgagcaca gccagcagca ggtgctcgct
     1801 gccgacccag ccgtcgccgc gggcgacggc accctcgcgg gcgcccgaca ccacggcgcg
     1861 ggcgtcctct gtgaaccgct cgaacatggt cagttccctc catgccgtgg actgccggcc
     1921 cgccgcgtgt ggccggagtg cttcttgtgc actgcctgtc tgctgactcc gagcagcgcg
     1981 ccgatggcgt cccaggacca gccttgcgct cgggcgctgc tcacctgcag tgcctccagc
     2041 tgctcggtca gccggcgcag cgcggccacg gcgcgcagcc cgaccgccgg gtcctggctg
     2101 gtcgcctgct cggcgacttc ggtctcagtg gtcatggctg tcaacgtacg ttgacgcgac
     2161 ccgacgtgtc aaccgttgtt gacgacctcg ccggcaactc gcctcgacgg cttctcgggc
     2221 gcgtcggtgc gggaccaggg gaggagccgc cggtacggtc gcttcccgaa caggtgttcg
     2281 gacgtcgtcc ggcgggaggt ggcgtggtgc gcgtgctcgg ggtggacccc ggcctgaccc
     2341 gttgcgggct cggcgtggtc gacggtgcgg tggggcggcc ggtccgcctc gtggccgtcg
     2401 acgtggtgcg gaccccaccc accgacgaca tcggggcccg gctgctcgcc atcgagcggg
     2461 cggtcgaggc gtggatccac gagcatcggc cggacgccgt cgccgtcgag cgcgtgttca
     2521 gccagcacaa cgtccgcacc gtgatgggca ccgcgcaggc cagcgcggtc gccatgctcg
     2581 tggccgcccg ccgcgggctg cccgtcgagc tgcacacccc cagcgaggtc aaggccgcga
     2641 tcaccggcga cggccgggcc gacaaggccg cggtcgccgc gatggtggcc cgggtgctgg
     2701 gcctgtcggc gccgccgcgg ccagccgacg ccgccgatgc gctggcgctg gcgatctgcc
     2761 acgtctggcg gggctccatc tcgggccggc tggccgcggc ggtcgcggcc gctccccgct
     2821 cgcgcatccc ggccggccct cgtgcccgcg gcggctgggg cagcgcctca ccggtccggg
     2881 gcggcgcctg gacggccacc gcggcggcgg tggcgatgac ccgatcgacc cccggcgtac
     2941 gacgtccgag ccctcgccgt gccgacccgc ccacctccgg agccgcctcg tgatcgcgtt
     3001 cgtccatggt cgggtcgccg cgctgggccc ggacacggcg gtcgtcgagg tcggcggggt
     3061 cggtctctcg gtgctgtgca ccgccgccac catcgggtcg ctgcgggtcg gcgagccggc
     3121 gcggctggcc acctcgttgg tggtgcggga agactcgttg accctctacg ggttcgccga
     3181 cgaggacgag cgggtcgtct tcgagctcgt gcagaccgcc agcggggtcg gcccccggct
     3241 ggcccaggcg atgctcgccg tccaccgccc cgatgacctg cgccgcgccg tggctaccga
     3301 cgacctggtc gcgctgacca aggtgccggg gatcgggcgc aagggtgccc agcgcatcgt
     3361 cctcgagctc aaggaccggc tgggtccacc gcacggcctg accgcggcgc cgcgccggtc
     3421 gagcgatgac gggtggcgcg cccaggtgca cgccgggctg gcgagcctcg gctggtcgac
     3481 ccgcgaggcc gacgacgcgg tcgccgcggt cgaggagcag gcaagcacac tgggcgccga
     3541 cggtgccgcc cccgacgtgg ccacgctgct gcgggccgcg ctgcgcaccc tggaccgctc
     3601 gtgagcgagg acccgcgcga gctggtccac cccgaggcca gttccgacga gcgcgcgatc
     3661 gaggccgcgc tgcggccacg gtcgctgacc gagttcgtcg ggcaggagcg ggttcgcgag
     3721 cagctggggc tggtgctcga ggccgcccgg cgccgcggcc gggtgcccga ccacgtcctg
     3781 ctgtcggggc cccccgggct gggcaagacg acgctggcca tgatcatcgc agccgtgatg
     3841 caggccccgc tgcgggtcac cagcggcccg gccatccagc acgccggcga cctggcggcc
     3901 gtgctctcct cgctgaccga gggtgaggtg ctcttcctcg acgagatcca ccggatgtcc
     3961 cggccggccg aggagatgct ctacatggcg atggaggact tccgcgtcga cgtcgtcgtc
     4021 ggcaaggggc ccggggccac cgcgatcccg ctggagatcc cgccgttcac cctggttggg
     4081 gcgaccaccc gggcggggct gctgcccggc ccgctgcgcg accggttcgg gttcaccggg
     4141 cacatggact tctacacccc ggccgagctc gagcacgtgc tcgaccgcag cgcacggctg
     4201 cttggggtcg agctgcaccc cggaggggcg caggagatcg ccgggcgctc ccgtggcacc
     4261 ccccgcatcg ccaaccggct gctgcgccgg gtgcgcgact gggcccaggt gcgcggcgac
     4321 ggagtggttc accgggccgc tgcccgggcc gcgctcgagg tctacgaggt cgacgagctc
     4381 gggctcgacc ggctcgaccg ctcggtgctc ctcgcgctga tcgagcgctt cggcggcggc
     4441 ccggtcgggc tgggcaccct ggcggtcgcg gtgggggagg agcgcgagac ggtcgaggag
     4501 gtcgccgagc cgttcctggt gcgcgagggc ctgctggccc gcaccgcccg cggacgcgtc
     4561 gccaccgccg ccggctgggc ccacctcggg ctgcgtccac cccagccggc cgccaccctg
     4621 ttcgggtcgc tgggcgaggc tgatccgctc gagcgcggcg acgacgacga gtccgccgac
     4681 gtcgcccggt gagcgtcgcg ttggctctgc gggcgacccc tcgcctagac tcgcgcggga
     4741 tctcgtccgg tcgtgcggtc aggcgggccc tcggcaccac gcgcacatcc accgcacgcc
     4801 ggcgaccggc ggtctctgtc cggccgggcg ccggggcggc acctgccgct gcacccgatg
     4861 acaaaggatg acgtgacgtg gaaggcaatg ggctggtgag cgcaaccgtg ctcgcggcaa
     4921 atgaccaggg cggcggcagc tcggtcacca tcatcatgct gctgctgctg gcggtggcgt
     4981 tctacttcct catcctgcgg ccacagcgca cgcgggcgcg ggaggccgcc aagctgcagt
     5041 cgagcctggc ccccggccag acggtcatga ccagcagcgg gctctacggg cgcatcgaga
     5101 gcctcgagga cgacgtcgtc gtgctcgagg ccgcgccggg gctccagctg cgctgggcaa
     5161 aggcggcgat cgcccgcgtg gtgacgccag tcgccgactc gggctccgac ccgctcgaca
     5221 ccgggcacta acccagcccc cgcagacgtc ctacgggcgt cggcgcccgc gtgtcgaccg
     5281 gcgtacaacg cgctttttgc acacaccgac aggagtgagc tcaccgtggc agcacccagc
     5341 gcatcccgac ctggtcgggc agtcgctgcc ctcatcgtgg tcatcgcact gatctatggc
     5401 gccatcgcgg cgggggtggc gtggggcaac gggcagtgga cc
//
LOCUS       DATIBB010000154         2004 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_97802, whole genome shotgun sequence.
ACCESSION   DATIBB010000154 DATIBB010000000
VERSION     DATIBB010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2004
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97802"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1218)
                     /locus_tag="VLC50_05885"
     CDS             complement(<1..1218)
                     /locus_tag="VLC50_05885"
                     /inference="COORDINATES: protein motif:HMM:NF040070.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6350 family protein"
                     /protein_id="HSF27031.1"
                     /translation="MPEPPRPTRPAAGRRRAPADPRPVAAAPAAGVVAALTAAALSLL
                     VATVAVLVAWVSATRTEIGGGDALRTAVQTWLLAHHGGLQLGGAGAGDGSGFLGLVPL
                     GLTALPAVLLAAAAARAVRMMHVRTLRGGLPLYLALVASYAVVPVALAPLADTDDVRP
                     LASQAFLGALVLSALAGGYGVLRGAGLWPSLRRSMPPMVRAVTAAATAATALLLGAGA
                     VLAAASLGLHAGQVTELVRSLDPGPAGGVVLLLTGVMLVPNAVVWAAAYAIGPGFAVG
                     AGTSVGPFAVHLGAVPAFPLLGALPSEPAMPLVALVVLLTPFAAGVVAGIIVVRRTGW
                     LPERAQTGWAAVAGVVSGMVLGVLAALSGGPVGGGRMATVGPSPWQVGLFAALEIGLA
                     GALTAWALPAWLRA"
     gene            1381..1947
                     /locus_tag="VLC50_05890"
     CDS             1381..1947
                     /locus_tag="VLC50_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020576916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SigE family RNA polymerase sigma factor"
                     /protein_id="HSF27032.1"
                     /translation="MSTLVRREGWVVRGPSTDEGAGASASESSAASFDAFYLATRARL
                     GHQLYAMTGNLHDAQECVQEAYARAWQRWGQVSTYDDPAAWVRTVAWRIAVNRWRRTK
                     SRMAAMVRHGAPDPVPEPAVDHVALVAALRQISEAQRRAIVLHHLCGLSVEDVAHETG
                     APVGTVKARLSRGRAALAALIVEEDPRG"
BASE COUNT          288 a          766 c          772 g          178 t
ORIGIN      
        1 ggcccgcagc caggccggca gcgcccacgc ggtcagggcg cccgcgagcc cgatctccag
       61 cgcggcgaac agcccgacct gccagggcga gggcccgacg gtggccatcc gaccgccgcc
      121 gaccggcccg cccgagagcg cggccagcac gccgagcacc atgccgctca ccacgccggc
      181 gacggcggcc cagccggtct gcgcgcgctc gggcagccac ccggtccgtc gtacgacgat
      241 gatcccggcc acgaccccgg cggcgaacgg cgtgagcagc accaccagcg cgaccaacgg
      301 catggccggc tcgctgggca gggcaccgag cagcgggaac gccgggaccg cgcccaggtg
      361 cacggcaaac gggccgaccg acgtgccggc cccgaccgcg aacccgggcc cgatggcata
      421 ggcggcggcc cagaccaccg cgttgggcac cagcatcacg ccggtcagca ggaggaccac
      481 cccgcccgcc gggccagggt cgaggctgcg gaccaactcg gtgacctgcc cggcatgcag
      541 gccgagcgag gccgcggcca gcacggcgcc ggcgccgagc agcagggcgg tcgccgcggt
      601 cgccgcggcg gtaaccgcgc gcaccatcgg cggcatcgac cggcgcaggg agggccacag
      661 acccgcgccg cgcaagacgc cgtagccgcc ggccaacgcg ctgagcacga gcgcgcccag
      721 gaaggcctgg gaggccagcg gacgcacgtc gtcggtgtcg gccagcgggg ccagcgcgac
      781 cgggacgacg gcatagctgg cgaccagggc caggtagagc ggcagcccgc cgcgcagggt
      841 gcgcacgtgc atcatgcgga ccgcccgggc ggccgcggcc gcgagcagca ccgccggcaa
      901 cgcggtcagc ccgagcggga cgaggccgag aaaccctgag ccgtcaccgg ctcccgcacc
      961 gccgagctgc aggccgccgt ggtgggcgag caaccaggtc tgcaccgcgg tccgcagcgc
     1021 atcaccgccg ccgatctcgg tgcgggtcgc gctgacccag gccaccagca cggccaccgt
     1081 ggcgacgagc aggctcagcg ccgcggccgt cagggcggcg acgacacccg cggccggcgc
     1141 tgcggcgacc gggcgcgggt cagcgggtgc gcgacggcgc ccggcggcag gtctggtggg
     1201 gcggggcggc tcgggcatca ccggtcatgg tcccgtggcc cgcaccggcg gcggcgtacc
     1261 gggtgcctgt cggggtgtcg cgcatcgctc ggtcacgacc tcacgcaacc tggacccctg
     1321 ccggcgggtg agtgagggta gaccggcgca ccgggcgccg cggcgggaca gggacgagcc
     1381 gtgtcgacac tggtccgacg agaggggtgg gtggtgcgag gtccgtccac cgacgagggg
     1441 gccggggcct cggcatcgga gtcgtcggcg gcgtccttcg acgcgttcta cctcgccacc
     1501 cgcgcccgcc tcggccacca gctctatgcg atgaccggca acctgcacga cgcgcaggag
     1561 tgcgtgcagg aggcctacgc ccgggcctgg caacggtggg gccaggtgtc gacgtacgac
     1621 gacccggcgg cctgggtccg caccgtcgcc tggcgaatcg cggtcaatcg gtggcggcgt
     1681 acgaagtcgc ggatggcggc gatggtgcgc cacggcgcgc ccgacccggt gcccgagccg
     1741 gccgtcgacc acgtcgcgct ggtggcagcg ctgcgccaga tctccgaggc ccaacgccgg
     1801 gcgatcgtgc tgcaccacct gtgcggcctc tcggtcgagg acgtggccca cgagaccggc
     1861 gcaccggtgg gcaccgtcaa ggcccggctc tcccgcgggc gggccgccct ggccgcactc
     1921 atcgtcgagg aggacccccg tggctgagca cgacccgatc gccgaccggc tcgacgcgct
     1981 gcgcggcgac gtcgagacgc tgcg
//
LOCUS       DATIBB010000155         7460 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_1543050, whole genome shotgun sequence.
ACCESSION   DATIBB010000155 DATIBB010000000
VERSION     DATIBB010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 7460)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7460)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7460
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1543050"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            171..1151
                     /locus_tag="VLC50_05895"
     CDS             171..1151
                     /locus_tag="VLC50_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014447744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HSF27033.1"
                     /translation="MSTAANAPALGTAAPSRASRTGRAGRRSRGAGPQLYDFRRPTKL
                     SREHVRSLQILCETFGRRWSTLLTTSLRAVCGVSLLSIEQLTYDEYVSALSTPTVMNL
                     LSVEPIPGTGVIEISLPSAMATIDHLLGGPGGENQPARHLTEIETALLHGVLTRVLGE
                     LTYAFESLLPMRPELTGIEYNPQFAQAAAASDMVIVASFEMRIGALESVATLCLPFAG
                     VVARLESSHGHGVVTDRERRAREQAAVALASGLETVPVDVSVRFQPVPLTPAELVALA
                     VGDVLPLRHPTSAPLAVTAADVVFAHAVPGSQGKRLACLVVEPPEGEASA"
     gene            1157..1879
                     /gene="fliN"
                     /locus_tag="VLC50_05900"
     CDS             1157..1879
                     /gene="fliN"
                     /locus_tag="VLC50_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015801162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HSF27034.1"
                     /translation="MSTTAVSESLVAAAQAAAAHLPAPAELNVGEPASDPAAASLPAD
                     AAVAVCARLTGAVVGEVVVVVARELVDALAQSPLGALEVAPAVQPALDAAAAALGQVQ
                     AEGGRELPVDVALSSLTGKGVVAIVPLLADGAVHATVAFALTPAATPRTRPASHSAGL
                     ELLRDVEMEVTAELGRTRMTVRELLSLTPGAVVELDRAAGSPADLLVNGTLIARGEVV
                     VVDEEFGIRITEIVAPAESGVA"
     gene            1884..2288
                     /gene="fliO"
                     /locus_tag="VLC50_05905"
     CDS             1884..2288
                     /gene="fliO"
                     /locus_tag="VLC50_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014447742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HSF27035.1"
                     /translation="MTGQLIQVLASLGLVLLLIWAAARVLRRTAHGKATGALEVLARQ
                     PLSRTASLAVVRVADRALVVGVAEGRVSLLTETDLASVRTTEPVRETREPVLTSAAST
                     PESPSRLAGSALSPATWRTAVEVLRERTARRA"
     gene            2351..3226
                     /gene="fliP"
                     /locus_tag="VLC50_05910"
     CDS             2351..3226
                     /gene="fliP"
                     /locus_tag="VLC50_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011981709.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HSF27036.1"
                     /translation="MLALSVLTGSAASAATAPASYAGMSAPASVVAAPVTSYGASLVA
                     APAAPGPSTPAEPTAPADPAATTGGGTVTVDLNGINGKPSSSIVVILAITLLSVAPAL
                     LLMTTSFTKIIVVLSLTRNALGLQNTPPNQVLAGLALFLSLFVMAPVLGQVNELGVQP
                     YLHGTPVSVAFDDGVQPLKEFMLDHTRKDELALMSQAAHRDLPAARADVPLTTLVPAF
                     VLSELKSAFIIGFVIFVPFLVIDIVISASLMAMGMMMLPPVVISLPFKLLLFVMVDGW
                     GLIVTSLISSYRGGG"
     gene            3223..3498
                     /gene="fliQ"
                     /locus_tag="VLC50_05915"
     CDS             3223..3498
                     /gene="fliQ"
                     /locus_tag="VLC50_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HSF27037.1"
                     /translation="MTDTSVIEIAMQAMIISAKLGAPILITALAVGFGISLFQAATQI
                     QEVTLSFVPKIIAVAVVVLLSGNWMLHELISFTQQLFDMLPRLLGGG"
     gene            3501..4277
                     /locus_tag="VLC50_05920"
     CDS             3501..4277
                     /locus_tag="VLC50_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018685590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HSF27038.1"
                     /translation="MTLELAEPTVVALLLGSIRVAAWLLIAPGLGAKWIPAPVRALLS
                     VALVLPVLPALAEHAPPPTTAALVSAALVQVAVGAAMGFLTYIVFMAVQSAGDLIDIS
                     SGYSLASAFDPMSFQQNSVFGRLHQMIAMVLLFVLNLHLVLLRGVLTSFEALPLDAGP
                     TSLPWDSLGALLTDGIGRMFLAAVEIAGPLIAVLFLADVGLALLTRVAPSLNAFSIGF
                     PVKMLVALLLVGMTFPMLPSVLDNLVGTGNKAVAAFLGLT"
     gene            4274..5386
                     /locus_tag="VLC50_05925"
     CDS             4274..5386
                     /locus_tag="VLC50_05925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018685589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HSF27039.1"
                     /translation="MSASGAGGGEKTEKPTPKKLKDARKEGQVRRTPDVGAWAGILAA
                     VMIFPPLLGHVGEQVSTLLVRAVALVHDPAPGPAVALLGDGLLDAVISSLLLGAPLLV
                     IALATNIAQDGLHVASKKLKPDLKRLNPLEGFKRIAGPQALWELVKALLRSAVVGLLV
                     WQAVAGLVPVLVASGHLPLSAVLEALGDSLVSLLRGGAAAGLIMALADYFVVRRRVGK
                     QLKMSHQEIKEEMKQTEGDPHVKGAIRSRQLAMSRNRMISDVAEADVVLVNPTHVAVA
                     LRYDPARGAPRVVAKGAGAIAARIREEAGVHRVPMVADIPLARALYRACEVGTEIPPE
                     LFTAVARVLAFVLSLRSRGSAAGLHHLPVAATAAPR"
     gene            5588..6610
                     /locus_tag="VLC50_05930"
     CDS             5588..6610
                     /locus_tag="VLC50_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020509394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone reductase"
                     /protein_id="HSF27040.1"
                     /translation="MKAIIYSRTGGPDVLELVDRPTPDPGAGEVRVRISVSGVNPTDW
                     KVRRGSAPDQPLAVPEQVPGQDGAGVVDAVGSGVDGLTVGQRVWLWESAFGRAAGTTA
                     EYVVLPAAHVVPLPEGASLDLGASLGIPAITAHRCLTMAEGVPARLAPGALAGRTVLV
                     AGGAGAVGHAAIELARWAGATVVATVSSPAKAALASAAGAHHVVDYRAGDPAAAIREV
                     APDGVDIVVEVAVAANAELDRAVLATNGAVVAYGGAPGDAVNVPVRDLMVANTRLQFV
                     LVYTMPAAAKREAVAAVSAAVEAGALRVGEEAGLPLHRFPLERAADAHAAVAAGAVGK
                     VLIDIA"
     gene            6864..>7460
                     /locus_tag="VLC50_05935"
     CDS             6864..>7460
                     /locus_tag="VLC50_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012947160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHIPEP family type III secretion protein"
                     /protein_id="HSF27041.1"
                     /translation="MMVVPLPTFVLDLLLAANITGSLLILLVCMNVKRALDFSVFPAL
                     LLVATLFRLALNVSATRLVLLDGYAGKVIDAFGHFVVGGSLVVGLVIFLILIVIQFVV
                     ITNGAGRVAEVGARFTLDAMPGKQMAIDADLNAGLIDEETARRRRAEVAAEADFYGAM
                     DGSSKFVKGDAIATIIITAINLVGGLVIGVVQRGLPLGE"
BASE COUNT          894 a         2721 c         2686 g         1159 t
ORIGIN      
        1 aatcagcacg gcgtacgacg gagccgtcat ggacctctac ttcaccgagt tcgtcatgca
       61 gtagccccca ccgccctgca ccccaccggg gtggatccct tgcgggtcac ccgggtggag
      121 cagtgccgcg ttttcctcag ccggccgggg cgtcggccga acactgcggc gtgagcaccg
      181 ccgcgaacgc gcccgccctg ggcaccgccg cgccctcacg cgcctcgcgc accgggcggg
      241 ccgggcgccg ctcgcgcggt gccggcccgc agctctacga cttccgccgt ccgaccaagc
      301 tctcccgtga gcacgtgcgc tcgctgcaga tcctctgcga gacgttcggt cggcgctggt
      361 ccacgctgct gaccacgtcg ctgcgcgcag tctgcggggt gtcgctgctc tcgatcgagc
      421 agctgaccta cgacgagtac gtcagcgcgc tgagcacgcc gaccgtgatg aacctgctct
      481 cggtcgagcc gatcccgggc accggggtca tcgagatctc cctgcccagc gcgatggcca
      541 ccatcgacca cctgctcggc gggcccggcg gcgagaacca gccggcccgc cacctcaccg
      601 agatcgagac ggcgctgctg cacggggtgc tgacccgggt gctgggcgag ctcacctacg
      661 cgttcgagtc gctgctgccg atgcgccccg agctgaccgg catcgagtac aacccgcagt
      721 tcgcccaggc agccgcggcc tccgacatgg tcatcgtcgc ctccttcgag atgcgcatcg
      781 gcgcgctcga gtcggtcgcc acgctgtgtc tgccgttcgc cggggtggtc gcccggctcg
      841 agtcttccca cgggcacggc gtggtcaccg accgggagcg ccgggctcgg gagcaggccg
      901 cggtcgcgtt ggcaagcggc ctcgagacgg tgccggtcga cgtgtcggtg cggttccagc
      961 cggtgccgct gaccccggcc gagctcgtcg cgctcgcggt cggcgacgtc ctgccgttgc
     1021 gccaccccac gtcggcgccg ctcgcggtca ccgcggccga cgtggtcttc gcacacgccg
     1081 tacccggcag ccagggcaag cggttggcct gcctggtcgt cgagcccccc gagggagagg
     1141 cctccgcatg accccgatga gcaccaccgc cgtcagcgag tcgctggtcg ccgcggccca
     1201 ggcggccgcc gcgcacctgc cggcccccgc cgagctcaat gtcggcgagc cggccagcga
     1261 ccccgccgcc gcgagcctgc cggccgacgc ggcggtggcc gtctgcgccc ggctgaccgg
     1321 cgcggtggtc ggcgaggtcg tcgtcgtcgt ggcccgcgag ctggtcgacg cgctggccca
     1381 gagcccgctc ggcgcgctcg aggtcgcccc ggccgtgcag ccggccctcg acgcggcggc
     1441 tgccgcgctg ggccaggtgc aggccgaggg tgggcgcgag ctgccggtcg acgtggcgct
     1501 gagctccttg accggcaagg gagtcgtggc gatcgtgccg ctgctcgccg acggtgcggt
     1561 gcatgccacc gtggcgttcg cgctcacgcc ggcggccacc ccgcggaccc ggccggcgtc
     1621 acactcggcc gggctcgagc tgctgcgtga cgtcgagatg gaggtcaccg ccgagctggg
     1681 ccgcacccgg atgaccgtgc gggagctgct ctcgctgacc cccggtgcgg tcgtcgagct
     1741 cgaccgggcc gccggcagcc ccgccgacct gctggtcaac ggcaccctga tcgcccgggg
     1801 cgaggtggtc gtcgtcgacg aggagttcgg catccggatc accgagatcg tcgcgcccgc
     1861 cgagtccggg gtcgcgtgac ctggtgaccg gtcagctcat ccaggtcctc gcctcgctcg
     1921 ggctcgtcct gctgctcatc tgggcggccg cccgggtctt gcggcgtacg gcgcacggca
     1981 aggcgaccgg cgcgctggag gtgctggccc gccagccgtt gtcgcgcacc gcgtcgctgg
     2041 ccgtcgtacg ggtcgccgac cgggcgctgg tcgtcggcgt cgccgagggg cgggtgtcgc
     2101 tgctcaccga gaccgacctg gcgtcagtgc gcacgaccga gccggtccgc gagacccgcg
     2161 agcccgtgct gaccagcgcc gcgtcgacgc cggagtcacc cagccggctg gccgggtcgg
     2221 cgctctcgcc ggcgacctgg cggaccgcgg tcgaggtgct gcgcgagagg acggcccggc
     2281 gggcatgact cggtcgctgc gtgcggcagt gtcgttgcgt gcggcagtct tcctgctcgt
     2341 agcagcggcc gtgctcgccc tgtcggtgct caccggctcc gccgcctcgg cggccaccgc
     2401 gcccgcgtcc tacgccggga tgagcgctcc cgcgtcggtc gtcgccgcgc cggtgacgtc
     2461 gtacggcgcc tcgctggtcg ccgcaccggc cgcacccggg ccgagcaccc cggccgagcc
     2521 caccgccccg gccgacccgg ccgcgaccac cggcggcggc acggtcaccg tcgacctcaa
     2581 cggcatcaac ggcaagccga gcagctcgat cgtcgtcatc ctcgcgatca cgctgctgtc
     2641 ggtggcgccg gcactgctgc tgatgaccac aagcttcacc aagatcatcg tggtgctgag
     2701 cctgacccgc aacgcgctcg gcctgcagaa caccccgccc aaccaggtgc tcgccgggct
     2761 ggcgctgttc ctcagcctgt tcgtgatggc cccggtgctc gggcaggtca acgagctcgg
     2821 tgtgcagccc tacctgcacg gcacgccggt ctcggtggcc ttcgacgacg gggtgcagcc
     2881 gctcaaggag ttcatgctcg accacacccg caaggacgag ctggcgctga tgagccaggc
     2941 ggcccaccgc gacctgccgg ccgcccgggc cgacgtgccg ctgaccacgc tggtgccggc
     3001 gttcgtgctc tccgagctca agtcggcctt catcatcggc ttcgtcatct tcgtgccgtt
     3061 cctcgtcatc gacatcgtga tcagcgcgtc gttgatggcg atgggcatga tgatgttgcc
     3121 gccggtggtc atctcgttgc ccttcaagct gttgctgttc gtcatggtcg acggctgggg
     3181 gctgatcgtc acgtcgctga tctcgagcta tcgaggtggc gggtgaccga cacctccgtc
     3241 atcgagatcg cgatgcaggc gatgatcatc tcggccaagc tcggcgcgcc gatcctgatc
     3301 acggcgctcg cggtcgggtt cggcatctcg ctcttccagg cggcgaccca gatccaggag
     3361 gtcacgctca gcttcgtccc caagatcatc gccgtcgcgg tcgtcgtact gctgtcaggc
     3421 aactggatgc tgcacgagct gatcagcttt acccagcagc tcttcgacat gctgccgcgc
     3481 ctgctcggcg gaggctgacg gtgacgctgg agctggccga gccgaccgtg gtggccctgc
     3541 tgctcggcag catccgggtg gccgcctggc tgctcatcgc ccccgggctg ggcgccaagt
     3601 ggatcccggc gccggtgcgc gcgctgctct cggtcgcgct cgtgctgccg gtgctgcccg
     3661 cactcgccga gcacgccccg ccgcccacca cggccgcgct cgtcagcgcc gcgctcgtgc
     3721 aggtcgcggt cggcgccgcg atgggcttcc tcacctacat cgtcttcatg gccgtgcagt
     3781 ccgccggcga cctcatcgac atctccagcg gctactcgct ggcctccgcg ttcgacccga
     3841 tgtccttcca gcagaactcg gtgttcgggc ggctgcacca gatgatcgcg atggtcctgc
     3901 tgttcgtcct caacctgcac ctggtgctgc tgcgcggggt gctcacgtcg ttcgaggcgc
     3961 tgccgctgga cgccggcccg acgagcctgc cctgggacag cctcggcgcg ctgctcaccg
     4021 acggcatcgg ccggatgttc ctggccgccg tcgagatcgc cggcccgctc atcgccgtac
     4081 tcttcctcgc cgacgtcggg ctcgcgttgc tgacccgggt cgccccctcg ctcaacgcgt
     4141 tctcgatcgg gttcccggtc aagatgctcg tcgccctgct gctggtcggc atgacgttcc
     4201 cgatgctgcc ctcggtgctc gacaacctcg tcggcaccgg caacaaggcg gtcgccgcct
     4261 tcctggggct gacgtgagtg ccagcggcgc cgggggcggt gagaagaccg agaagccgac
     4321 cccgaagaag ctcaaggacg cccgcaagga gggccaggtc cggcgtaccc ccgacgtggg
     4381 ggcctgggcc ggcatcctcg ccgcggtgat gatcttcccg ccgctgctcg ggcacgtggg
     4441 ggagcaggtc agcacgctgc tggtgcgcgc ggtcgcgctg gtgcacgacc cggcgcccgg
     4501 gccggcggtg gcgctgctcg gcgacggcct gctcgacgcg gtgatcagct cgctgctgct
     4561 cggcgcgccg ctgctcgtca tcgcgctggc gaccaacatc gcccaggacg gcctgcacgt
     4621 ggcgtccaag aagctcaagc ccgacctcaa gcggctcaac ccgttggagg gcttcaaacg
     4681 catcgctggc ccgcaggcgc tgtgggagct ggtcaaggcg ctgctgcgct cggcggtggt
     4741 cgggctgctg gtctggcagg cggtggccgg gctggtgccg gtgctggtcg cctccggtca
     4801 cctgccgctg tcggcggtgc tcgaggcgct cggcgactcg ttggtctcgc tgctgcgcgg
     4861 cggcgcggcg gccggtctga tcatggcgct ggccgactac ttcgtcgtac ggcggcgggt
     4921 gggcaagcag ctgaagatgt cgcaccagga gatcaaggag gagatgaagc agaccgaggg
     4981 cgacccgcac gtcaaggggg cgatccggtc gcgtcagctc gcgatgagcc gcaaccggat
     5041 gatctccgac gtggccgagg cggacgtcgt actcgtcaac cccacccacg tggcggtcgc
     5101 gctgcgctac gacccggctc ggggtgcgcc gcgggtggtc gctaagggcg ccggggcgat
     5161 cgcggcgcgg atccgtgagg aggccggcgt gcaccgggtg ccgatggtgg ccgacatccc
     5221 gctggcccgc gcgctctacc gggcctgcga ggtcggcacc gagatcccgc ccgagctgtt
     5281 caccgcggtg gcccgggtgc tggcgttcgt gctgtcgttg cgctcccgcg gctcggccgc
     5341 tggcctgcac cacctgcccg tcgccgcgac ggccgccccc cgctgacgcc gcccctgcac
     5401 tgatcatgcg cgtgtggcgg gcgccctccg caccgatcat gcgcgtccgg cggcggccgt
     5461 gccgcttggc aaggcgttgt ggcacttggc aaggcgtgca cgccttgcca agtaagcgaa
     5521 cgcccacttg gcgtggtcat tccccagggg gcgcgggggc tagttgatct tgcgccaagg
     5581 taggggcatg aaggcgatca tctacagccg caccggtggc cccgacgtcc tcgagctggt
     5641 cgaccggccg acccccgacc ccggcgccgg cgaggtgcgg gtccggatct cggtcagcgg
     5701 cgtcaacccg accgactgga aggtccgccg cggcagcgcg cccgatcagc cgctcgccgt
     5761 gcccgagcag gtgccgggcc aggacggcgc tggcgtggtc gacgcggtcg gctccggcgt
     5821 cgacggtctg acggtgggtc agcgggtctg gctgtgggag tcggcgttcg gccgggccgc
     5881 cgggaccacg gctgagtacg tcgtcctgcc ggccgcgcac gtcgtaccgc tgcctgaggg
     5941 tgcctcgctc gacctgggcg cgagcctggg catcccggcg atcaccgcgc accgctgcct
     6001 gaccatggcc gagggggtgc cggcccggtt ggcgccgggc gcgctggccg gacgcaccgt
     6061 gctggtcgcc ggtggggccg gcgccgtggg gcacgccgcg atcgagctgg cccgctgggc
     6121 cggcgccacg gtggtggcca cggtcagcag cccggccaag gcggcgctgg ccagtgccgc
     6181 cggggcccat cacgtggtcg actaccgggc cggtgacccg gccgccgcga tccgcgaggt
     6241 ggctccggac ggggtcgaca tcgtcgtcga ggtcgccgtc gcggccaacg ccgagctcga
     6301 ccgggcggtg ctggcgacca acggtgcggt ggtggcctat ggcggcgcgc ccggtgacgc
     6361 ggtgaacgtg ccggtgcgcg acctgatggt ggctaacacc cggctgcagt tcgtgctggt
     6421 ctacacgatg ccggcggcgg ccaagcgcga ggccgtggcg gcggtctcgg ctgcggtcga
     6481 ggccggcgcg ctccgcgtgg gcgaggaggc cggcctgccg ctgcaccggt tcccgctcga
     6541 gcgggcggcc gacgcgcacg ccgcggtggc ggccggcgcc gtcggcaagg tgctcatcga
     6601 catcgcctga ccgcgtcgtt ccatggtcat gaaggtctgc tgcccctagg agggcggcag
     6661 accttcatga tcactgccag gaggggagaa gggctcaggt cggcggggcg ggcgccgatg
     6721 gggacatcgc caggacggcg cccccggcca cggacgaggc catcgacgag cggtaaacgc
     6781 tcgcgttgac gtcggaaggt gccgtgctgc gcagccgggt gggccagatc ggtgtgccga
     6841 tcacggtcgt cgccatcgtc gtgatgatgg tcgtaccgct gccgacgttc gtcctcgacc
     6901 tgctcctcgc cgccaacatc accggctcgc tgctgatcct gctggtctgc atgaacgtca
     6961 agcgggcgct ggacttctcg gtgttcccgg cgctgctgct ggtcgccacc ctgttccggc
     7021 tcgcgctcaa cgtcagcgcc acgaggctgg tcctgctcga cggctacgcg ggcaaggtga
     7081 tcgacgcgtt cggccacttc gtcgtcggcg gctcgctggt tgtcggcctg gtcatcttcc
     7141 tgatcctcat cgtgatccag ttcgtggtca tcaccaacgg cgccggccgg gtggccgagg
     7201 tcggcgcccg gttcaccctc gacgccatgc ccggcaagca gatggccatc gacgccgacc
     7261 tcaacgccgg gctcatcgac gaggagacgg cgcggcgccg ccgggcggag gtggccgccg
     7321 aggccgactt ctacggcgcc atggacggct cgtccaagtt cgtgaagggc gatgcgatcg
     7381 cgacgatcat catcacggcc atcaacctcg tcggaggcct ggtgatcggc gtcgtccagc
     7441 gcggcctccc cctcggcgaa
//
LOCUS       DATIBB010000156         4192 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_1280189, whole genome shotgun sequence.
ACCESSION   DATIBB010000156 DATIBB010000000
VERSION     DATIBB010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 4192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4192
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1280189"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..468
                     /locus_tag="VLC50_05940"
     CDS             <1..468
                     /locus_tag="VLC50_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020575840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dehydratase"
                     /protein_id="HSF27042.1"
                     /translation="WQFCHVDDLVSALELAAHERVTGAVCVGCDGWLDHDEVERISGR
                     RSIELPSGVAFATAERLHRLGLIPAPASDLRYVVHPWVVSCDTLKAAGWRPSYDNATA
                     LEALLTEVSGRHTVAGHRLGRRDATLGAAGAAVAVVGTAALVRRARRRRRSGS"
     gene            465..893
                     /locus_tag="VLC50_05945"
     CDS             465..893
                     /locus_tag="VLC50_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011754988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HSF27043.1"
                     /translation="MSDAVRLVGIREEPLSTDEVLAAVGGRSAGGVVLFVGVVRDIDA
                     GHQVEALSYSAHPSAHDALTEVLEEVAGEYDVIALAATHRVGDLVVGDLATVVAASAV
                     HRGEAFEAAKACIDRLKARVPIWKHQVFDDGSEEWVGSPG"
     gene            997..1206
                     /locus_tag="VLC50_05950"
     CDS             997..1206
                     /locus_tag="VLC50_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018845580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF27044.1"
                     /translation="MATIAWLGIPLAATLLAIVWVVWVSRPRPPADMHETLASYERFK
                     AAMSGDTRRRWRRRPGSADRRRGQA"
     gene            1245..2357
                     /locus_tag="VLC50_05955"
     CDS             1245..2357
                     /locus_tag="VLC50_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018510090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HSF27045.1"
                     /translation="MPEQPTPRPDSSPRRRIGGRITLSRRTATLSLLALAVAVLTAVA
                     ALLPVPYVVLGPGPTENTLGTLGKTPLINIEGRTTYPAKGNLNLVTVLVSGGPGSHLD
                     LFTALRGWIDPDVAVVPVESVFAPDTTADQVRQRNAEEMTLSQQDATVAALHHLGIPV
                     TERVAVHAVLEGAPAEGRLKAGDIVLDVDGTKATNADVVRSTISAHRPGDEVAITVQR
                     DGKTLTERLTTIEDDGRTVVGFIPGALYTYPFTVEIDPGNIGGPSAGLMFSLGVIDKL
                     TPGDLTGGAFVAGTGTINVDGEVGPIGSIPQKMIGAREAGARWFFVPTKNCAEAVGAA
                     PDGLRLVKVSTLDDALDSLEAISTGRGLDALPACSS"
     gene            complement(2407..2952)
                     /locus_tag="VLC50_05960"
     CDS             complement(2407..2952)
                     /locus_tag="VLC50_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018536212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPA1309 family protein"
                     /protein_id="HSF27046.1"
                     /translation="MSAAQTSSPDPLVQVVTDLDRHAAQGSWDSPPRVYALVPTRELL
                     AREPGLAEREDLVTAAATAPDSLAPVEQDDMPAAPTLEESLARIAWPAEVVGAAVVLE
                     RIVLPAGLEAAMPADEQEALRWVATHPRREEVRLTVAVLRDGRRASAVRMRRHDEDAS
                     VLRGPDLAPALADHLAHTLTD"
     gene            3096..>4192
                     /locus_tag="VLC50_05965"
     CDS             3096..>4192
                     /locus_tag="VLC50_05965"
                     /inference="COORDINATES: protein motif:HMM:NF015644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HSF27047.1"
                     /translation="MSFDFPDPAQTPPRRPVAIKRRRTVLLPTVGVLIVLVLAFGLFA
                     GFWTDWLWFRTTGYTTVIRTEILTKIGLFLGFGVAFAAVVIGNFAIAYRLRPTYQIPT
                     PDAAVLERYRRSIEPFRRLIMIAGAIVLVLFAGSSASSAWRTWLQWRNRTSFGTKDPQ
                     FGLDVSFYVFSLPWWRFMLGFAFAALVVSLVVSLVAHWLYGGLRPQAPSGQRTTAAAR
                     VHLSVLLGVFVLLKAVGYWLDRYSLATAGSTVGREEFTGLRYTDVNAVLPGKTILAVI
                     AVICAVLFFATAITRSWLLPGVGVGLLVLSAVLVGGIYPAAVQYFQVRPSEPDREAPY
                     LTNFIAGTRTAYNIADTQVQQYAAKTTATAG"
BASE COUNT          535 a         1540 c         1500 g          617 t
ORIGIN      
        1 tggcagttct gccacgtcga cgacctggtc agcgcgctcg agctggcggc ccacgagcgg
       61 gtgaccgggg cggtctgcgt cggctgtgac ggctggctcg accatgacga ggtcgagcgg
      121 atcagcggcc ggcgcagcat cgagctgccc tccggggtgg ccttcgcgac cgccgagcgg
      181 ctgcaccggc tcgggctgat ccccgccccg gccagcgacc tgcgctacgt cgtccatccc
      241 tgggtcgtct cctgcgacac gctgaaggcg gccggctggc ggccgtcgta cgacaatgcg
      301 accgcgctcg aggcgctgct caccgaggtc agcggacgcc acacggtggc cgggcaccgg
      361 ctcgggcgtc gggatgcgac cctgggggcc gccggggcag cggtcgccgt ggtgggcacg
      421 gccgcgctgg tccggcgcgc ccgacgacgt cgaaggagcg gctcatgagc gatgcggtgc
      481 gcctggtggg gatccgggag gagccgctgt ccaccgacga ggtgctcgcg gccgtgggag
      541 gccgctcggc cgggggagtg gtgctgttcg tcggggtggt ccgagacatc gacgccggtc
      601 accaggtcga ggcgctgtcc tattccgcgc accccagcgc ccacgacgcc ctcaccgagg
      661 tcctcgaaga ggtcgccggc gagtacgacg tgatcgcgct ggccgccacc catcgggtcg
      721 gcgacctcgt ggtcggcgat ctggccacgg tggtcgccgc ctccgcggtg caccggggcg
      781 aggcgttcga ggcagccaag gcctgcatcg accggctcaa ggcccgggtg ccgatctgga
      841 agcaccaggt cttcgacgac ggcagcgagg aatgggtcgg gtcgccggga tagcggccgg
      901 cccgcgcgat gccgagtttt ggcagttgag cgcggttcgg cgcggttagg cttccgcggg
      961 gcacttcgac gagtcaggaa acgggcgaca cgaaccatgg cgaccatcgc gtggctgggc
     1021 atcccgctcg ccgcgacgct gctcgcgatc gtctgggtgg tctgggtcag ccgtccacgc
     1081 ccgccggccg acatgcacga gaccctggcc agctatgaac ggttcaaggc agccatgagc
     1141 ggtgacaccc gccgccggtg gcgccgccgc cccggcagcg ccgaccggcg ccgcggtcag
     1201 gcctgagcgc tccttgccga tcgacccgcc tttaggctgc acctgtgcct gagcagccga
     1261 cgccgcgtcc tgactcgtcg ccgcgccggc gcatcggcgg gcggatcacc ctgtcccgcc
     1321 gtacggcgac cctgtcgctg ctcgcgctcg ccgtcgccgt gctgaccgcg gtggccgcgc
     1381 tgctgcccgt gccctatgtc gtgctcggcc ccgggccgac cgagaacacc ctcggcaccc
     1441 tcggcaagac gccgctgatc aacatcgagg gacgcaccac ctatccggcg aagggcaacc
     1501 tcaacctggt cacggtgctc gtcagcggcg ggcccggcag ccacctcgac ctgttcaccg
     1561 cgctgcgtgg ctggatcgac cctgacgtcg cggtcgtgcc ggtcgagtcg gtcttcgcgc
     1621 ccgacacgac cgccgaccag gtgcgccagc gcaacgccga ggagatgacg ctctcgcagc
     1681 aggacgcgac ggtggccgcc ctgcaccacc tcggcatccc ggtgaccgag cgggtcgccg
     1741 tccacgcggt gctcgagggc gccccggccg agggccggct caaggccggc gacatcgtcc
     1801 tcgacgtcga cggcaccaag gcgaccaacg ccgacgtcgt ccgctcgacc atctcggcgc
     1861 accggcccgg tgacgaggtc gccatcaccg tccagcgtga tggcaagacc ctgaccgagc
     1921 ggctgaccac gatcgaggac gacggccgca ccgtggtcgg gttcatcccc ggtgccctct
     1981 acacctaccc gttcaccgtc gagatcgacc cgggcaacat cggggggccc agcgccgggc
     2041 tgatgttctc gctgggcgtc atcgacaagc tcacgccggg cgacctcacc ggcggcgcgt
     2101 tcgtcgccgg caccgggacg atcaacgtgg acggcgaggt ggggccgatc ggcagcatcc
     2161 cgcagaagat gatcggcgcc cgcgaggccg gtgccaggtg gttcttcgtg ccgaccaaga
     2221 actgcgccga ggccgtcggc gcggcccccg acgggctgcg gctggtcaag gtgtcgaccc
     2281 tcgacgacgc gctcgactcg ctcgaggcga tctcgaccgg gcggggcctg gacgccctgc
     2341 cggcctgctc cagctgacca gccgacgctg ccccgccggc ccgcgtccac agggggtctg
     2401 acggcgtcag tcggtcaggg tgtgcgcgag atggtcggcc agcgccgggg cgaggtcggg
     2461 tccgcgcagg accgaggcgt cctcgtcatg gcggcgcatc cggaccgcgc tggcccgacg
     2521 cccatcgcgc agcaccgcga cggtcagccg gacctcctcc cgccgggggt gggtcgccac
     2581 ccagcgcaac gcctcctgct cgtcggcggg catcgccgcc tcgaggccgg ccggcagcac
     2641 gatccgctcg agcaccaccg cggcgcccac cacctcggcc ggccacgcga tccgggccag
     2701 cgactcctcg agcgtggggg cggcgggcat gtcgtcctgc tcgaccggcg cgagcgagtc
     2761 gggtgccgtc gcggccgcgg tgacgaggtc ctcgcgctcg gccagccccg gctcgcgggc
     2821 gagcagctct cgggtcggca ccagcgcgta gacgcgcggc gggctgtccc aggacccctg
     2881 cgcggcgtga cggtcgaggt cggtgacgac ctggaccaac gggtcgggcg acgaggtctg
     2941 cgcggcgctc accggctcat cctcgcgcag cgccgtacgg cgacggcagg gtgggcgtgg
     3001 gacgtcggtt cgggaacttg gtctggactc ggtaagttga cggttcgacg cccggctagt
     3061 tcgagccggg cgcccgacct gcgaaggtgc cttcggtgag cttcgacttc cccgacccag
     3121 cacagacccc gccgcgtcgg ccggtggcga tcaagcgccg ccgcaccgtg ctgctgccga
     3181 cggtcggcgt gctcatcgtg ctggtgctcg cgttcgggct cttcgcgggc ttctggaccg
     3241 actggctgtg gttccggacg accggctaca ccacggtcat ccgcaccgag atcctcacca
     3301 agatcgggct gttcctcggg ttcggggtgg ccttcgccgc ggtggtcatc ggcaacttcg
     3361 cgatcgccta ccgcctgcgt ccgacctacc agatcccgac ccccgacgcg gcggtgctcg
     3421 agcgctaccg ccggtcgatc gagccgttcc ggcggctgat catgatcgcg ggcgcgatcg
     3481 tgctggtgct gttcgccggg tcgagcgcgt cctcggcctg gcgcacctgg ctgcagtggc
     3541 gcaaccggac ctccttcggc accaaggacc cgcagttcgg gctcgacgtg tccttctacg
     3601 tgttctcgct gccctggtgg cggttcatgc tcgggttcgc gttcgcggcg ctcgtcgtct
     3661 cgctcgtggt ctccctggtc gcccactggc tctacggggg actgcgcccg caggcgccgt
     3721 cggggcaacg gaccaccgcc gccgcgcggg tccacctgtc ggtgctgctc ggggtcttcg
     3781 tgctgctcaa ggcggtcggc tactggctcg accgctactc gctggccacg gccggctcga
     3841 cggtcgggcg cgaggagttc accgggctgc gctacaccga cgtcaacgcc gtgctgcccg
     3901 gcaagacgat cctcgccgtc atcgcggtga tctgcgcggt gctcttcttc gccacggcga
     3961 tcacccggtc gtggctgctg ccaggggtcg gcgtcgggct gctcgtcctg tcggcggtgc
     4021 tcgtcggggg catctatccg gccgcggtgc aatatttcca ggtccgcccg agcgagcccg
     4081 accgtgaggc gccctacctc accaacttca tcgccggcac ccgcaccgcc tacaacatcg
     4141 ccgacacgca ggtgcagcag tacgccgcca agaccactgc gaccgccggg ca
//
LOCUS       DATIBB010000157        10072 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_1807269, whole genome shotgun sequence.
ACCESSION   DATIBB010000157 DATIBB010000000
VERSION     DATIBB010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 10072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10072
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1807269"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1537)
                     /locus_tag="VLC50_05970"
     CDS             complement(<1..1537)
                     /locus_tag="VLC50_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013131566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HSF27048.1"
                     /translation="MSQRSTLPPASEQTPTYLSHRQILVVLFGVMSGMLLAALDQSIV
                     GVALPRITSELGGLDKLAWVVTAYLVTATAMTPLWGKISDLYGRRLIFQLAISIFLLG
                     SLLSGLSQDIIQLIAFRALQGIGGGGLFAIAFAIIGDVIPPRERGRYQGYFGAVFGVA
                     SVAGPLLGGWFTDGPGWRWIFYINLPVGIAALFITSSALKIPTVRREHSIDWLGAVTI
                     VASVSSLLLYLDWRGKNFGWGDPVGLALVGAAVVLALLFLLIEARTKEPIIPLHLFAN
                     RTFGIGNIFGFLAGFAMFGAIIFLPVYLQTAKGMTPTESGLAMLPAVLGIFSASIVAG
                     LLMTRTGRYKVFPVVGAVAIVVALLLMSTITTTMPYWRLGLMMYLFGTGLGMTMQTIV
                     TAIQNDVSMRDMGTATSATTFFRQLGGAIGTAVFGAVLTSRLTVYLVDSFAGAPAVAG
                     SGDIANDVQKIHALPEPIRAKVLDAFSAAIGDVFLVAAPILVIALVAALFLKEIPLKT
                     AAHG"
     gene            complement(1556..2008)
                     /locus_tag="VLC50_05975"
     CDS             complement(1556..2008)
                     /locus_tag="VLC50_05975"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HSF27049.1"
                     /translation="MQATPQRDELATAVFALAGRLKRMQYDEPVERAGLAVLHTLACR
                     AEPLRLSGVAAALSLDSSTVSRHVRSLEDAGLIGRTCDPDDRRAYRLTLTEQGAAALE
                     QAFDLRACTIDAALADWSPADRAQLTTLLTRLEADLGRRPESAPRTLS"
     gene            2077..3312
                     /gene="ilvA"
                     /locus_tag="VLC50_05980"
     CDS             2077..3312
                     /gene="ilvA"
                     /locus_tag="VLC50_05980"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018834605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - L-threonine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006549 - isoleucine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HSF27050.1"
                     /translation="MSTSIQPSLGGAVTLDDVLAARALLADIARVTPIEGSRPLSERV
                     GGPVFLKCENLQRAGSFKIRGAYTRIARLSADERARGVVAASAGNHAQGVAQAATLLG
                     TRSTVFMPAGAPIPKVAATKAYGAEVRFHGHTVDDALLGAEEFARATGAVLIHPFDHP
                     DVIAGQGTIGLEILEQCPDVRTIVAGVGGGGLVAGIAVAVKALRPDVRVVGVQAESAA
                     AYLPSLAAGKPVALDAMSTMADGIAVGCPGRLPFAIVQELVDDVVTVSEEFLSRALLL
                     VLERAKLVVEPAGAASVAALINAPTAFEPPVVAVLSGGNIDPLLLLRVLRHGLAAAGR
                     FLSLRVRVSDQPGALARLLATLAQADASVLEVEHVRTDPKLSLDEVELALQLETRGIE
                     HSEELLATLRAAGYRLTFS"
     gene            3476..4486
                     /locus_tag="VLC50_05985"
     CDS             3476..4486
                     /locus_tag="VLC50_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018381893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HSF27051.1"
                     /translation="MADDPAIYAEDLVKTYGEVRALDGVDLAVAQGTVLGVLGPNGAG
                     KTTAVRILTTLLLPDSGRASVAGFDVVREAAALRQVIGLSGQYAAVDEHLTGRENLDM
                     VGRLYHLGRAVSRQRAGELLERFDLAEAADRPVKTYSGGMRRRLDLAGALVATPRVLF
                     LDEPTTGLDPRSRLQMWDVIAELVGGGTTLMLTTQYLEEADRLADRIAVIDRGKVIAR
                     GTADELKTQVGGERLEVVVGADHDLAKATSVLAEVGQEAPAVEPHARRLTVPVIGGTP
                     VLIEALRRLHSNGVEVLDVAIRRPTLDDVFLTLTGHAAEDEDDDTTTAAAAGGRGRRR
                     DK"
     gene            4483..5295
                     /locus_tag="VLC50_05990"
     CDS             4483..5295
                     /locus_tag="VLC50_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015099189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSF27052.1"
                     /translation="MSQLVSALGDGLVVTKRNLIKIKRVPDLLVFTTMQPIMFVVLFA
                     YVFGGAIAVEGVNYREFLMAGIFAQTVIFGATLTGAGLADDMQKGVIDRFRSLPMSRS
                     AVLVGRTFSDMLNNVIVIIVMAATGLVVGWRIHTSFLEALGGFALLLLFSYAFSWVMA
                     YVGLIVPSVEVVNNAAFIVLFPVTFIASTFVPLASLPGPLETVAAWNPVTTLSESGRL
                     LFGNIPADPNLRNLSDAWSMQNPVFYTVICAALILVVFVPLSVHKYRSATTK"
     gene            complement(5355..5843)
                     /gene="greA"
                     /locus_tag="VLC50_05995"
     CDS             complement(5355..5843)
                     /gene="greA"
                     /locus_tag="VLC50_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007826394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HSF27053.1"
                     /translation="MTEATSSVTWLTQEAYDRLKAELDQLTGLGRSEIVKRIEAAREE
                     GDLRENGGYHAAKEEQGKQEARIRQLQTLLQHAKVGQAPPDDGVVEPGMVVTAKVAGQ
                     EMTFLLGSREASGGELDVYSERSPLGEAINGKHTGDTATYTAPNGKQVTVEILDAKPY
                     SG"
     gene            complement(5951..6334)
                     /locus_tag="VLC50_06000"
     CDS             complement(5951..6334)
                     /locus_tag="VLC50_06000"
                     /inference="COORDINATES: protein motif:HMM:NF025520.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4307 domain-containing protein"
                     /protein_id="HSF27054.1"
                     /translation="MARYDEDAHPLRHMLLVMGAALLAAIALGWFLWSAAYHAAPDAT
                     TRLIGYSVTGPDAVQVRYEVTRDPAEAVQCTLEAQDRFHDSVGRLTVDVPAGGEATVA
                     RADVVRTTAPAVTGLVLGCDPAAGS"
     gene            6558..7517
                     /gene="mca"
                     /locus_tag="VLC50_06005"
     CDS             6558..7517
                     /gene="mca"
                     /locus_tag="VLC50_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007074698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010126 - mycothiol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mycothiol conjugate amidase Mca"
                     /protein_id="HSF27055.1"
                     /translation="MTERLRLMAVHAHPDDESSKGAATAARYVDEGVEVLVVTCTGGE
                     RGSILNPRLAHDPAVLANLAEVRRSEMDAARAILGVEQAWLGFVDSGLPEGDPLPPLP
                     EGCFALTPLEVAAEPLVRLVREFRPHVMTTYDENGGYPHPDHVMCHRVSVEAYHAAAD
                     PARYPGTGDPWATPKLYYMQTMSLARTLALHESMLAAGLDSPFAERIERYRGREPRTI
                     TTRVPCADWFPVRDRALLAHATQVDPDGAWFACPMDIQRDVWPTEDYELAHSRVATDL
                     PEDDLFAGVRELVPGTVTGPDADLDTDFDTDLDTDLDTDKELA"
     gene            7514..7849
                     /locus_tag="VLC50_06010"
     CDS             7514..7849
                     /locus_tag="VLC50_06010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF27056.1"
                     /translation="MSAMVTLGLAPTGAAVAVPTAVLADVDPAKVQTGAIGMVVFLLL
                     CGATALLLRSFRNQMRKVEQADLPHEKPRPHGPRPGIRLPSAEPEPGSSPEPQPGSQP
                     GPNGEHPRG"
     gene            7975..8406
                     /locus_tag="VLC50_06015"
     CDS             7975..8406
                     /locus_tag="VLC50_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020489468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HSF27057.1"
                     /translation="MTRRRRPEASLWSEFMGWPESVPFGELFRAMRGEGIRVEEYVDD
                     GTLVIRADLPGIDPEKDVDVSVSNGMLRIKAERQESKKGETASGYRSEVHYGSLSRVM
                     PLPGGVTEKDVTATYSDGVLEIRLPMATEEETTHSIPIQRG"
     gene            8440..>10072
                     /locus_tag="VLC50_06020"
     CDS             8440..>10072
                     /locus_tag="VLC50_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009742003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HSF27058.1"
                     /translation="MPNRLATATSPYLLQHKDNPVDWWPWSDEAWAEAVRRDVPVFLS
                     VGYAACHWCHVMAHESFEDPEVAAFLAEHFVSIKVDREERPDVDAVYMQATQALTGQG
                     GWPMTVFASPAGEPFYAGTYFPPRPAHGLPSFRQLIEAIAASWRERRAEVVSASESIT
                     AQLAAVRPAGSGQTPLTAELLDTAVAALAREYDATHGGFGGAPKFPPSMVLEFLLRQH
                     ARTGASESLAMVAGTCEAMARGGVYDQLGGGFARYGVDRAWVVPHFEKMLYDNALLLR
                     VYLHWWRATGASLAERVVRETAEFLLRDLRTPEGGFAASLDADTLVETAHGPRGVEGA
                     TYVWTPEQLRAVLGDDDGAWAAALLEVTDAGTFEHGASTLQLLRDPDDSARWDVVRRR
                     LLAARAARPQPSRDDKVVAAWNGLAIAALAEAGALLDEPAYVEAAVAAADLLVTVHLD
                     ERGRLRRTSRDGRVGDSAGLLEDHADVAEGLLALHQVTGDPVWLSLADGLLAVVLADF
                     ADADGLLYDTALERTDPRVLRLGRPADPTDNATPSG"
BASE COUNT         1425 a         3598 c         3593 g         1456 t
ORIGIN      
        1 ggccgtgcgc ggcggtcttg agcgggatct ccttgaggaa caaggccgcc accaatgcga
       61 tgaccaagat cggcgcggcg acgaggaaca cgtctccgat cgccgcgctg aacgcgtcga
      121 gcaccttggc acggatcggc tcgggcagcg cgtggatctt ctgcacgtcg ttggcgatgt
      181 cgccggagcc cgcgacggct ggtgccccgg cgaacgagtc gacgaggtag accgtcagcc
      241 ggctggtcag caccgctcca aacaccgcgg tgccgatggc gccgccgagt tggcggaaga
      301 acgtcgtcgc actcgtcgcc gtgcccatgt cacgcatgct cacgtcgttc tggatcgcgg
      361 tgacgatcgt ctgcatcgtc atgcccaacc cggtgccgaa gaggtacatc atcagcccga
      421 gccgccagta gggcatggtc gtggtgatgg tcgacatcag cagtagggcg accacgatgg
      481 cgaccgcgcc gacgaccggg aagaccttgt agcggccggt acgggtcatc agcagcccgg
      541 ccacgatcga ggccgagaag atgccgagga cggccggcag catggccagc ccagactcgg
      601 tcggcgtcat gcccttggcg gtctgcaggt agaccggcag gaagatgatc gcgccgaaca
      661 tcgcgaagcc ggccaggaac ccgaagatgt tgccgatgcc gaaggtccgg ttggcaaaca
      721 ggtgcagcgg gatgatcggt tccttggtcc gggcctcgat gagcaagaac agcagggcaa
      781 gtacgacggc tgcgccgacc agcgcgagcc cgaccgggtc accccagcca aagttcttgc
      841 cgcgccagtc caggtaaagc agcaggctcg acaccgacgc gacgatggtg accgcgccga
      901 gccagtcgat gctgtgctcg cgccggaccg tcgggatctt cagcgccgac gacgtgatga
      961 acagcgcggc gatgcccacc ggcaggttga tgtagaagat ccagcgccag cccggcccgt
     1021 cggtgaacca gccgcccagc agcggcccgg cgacgctggc cacgccgaag accgcgccga
     1081 agtagccctg gtagcgaccg cgctcgcggg gcgggatgac gtcgccgatg atcgcgaacg
     1141 cgatcgcgaa caggccgcca ccgccgatgc cctgcagggc ccggaaggca atgagctgga
     1201 tgatgtcctg gctcagcccg gacagcagtg agccgagcag gaagatgctg atcgcgagct
     1261 ggaagatcag ccgccggccg tagaggtcgg agatcttgcc ccacagcggg gtcatcgcgg
     1321 tggcggtgac gaggtaggcg gtcaccaccc aggcgagctt gtcgagcccg ccgagctcgc
     1381 tggtgatccg gggcagcgcc acgccgacga tgctctggtc gagcgcggcc aggagcatgc
     1441 ccgacatcac gccgaagagc acgacgagga tctgccggtg tgagaggtag gtcggcgtct
     1501 gttcggacgc aggcggcaac gtgctgcgtt ggctcatgcg ggacagtcct tcgcgtcagg
     1561 agagggtgcg cggggcgctc tcggggcgac gaccgaggtc ggcctcaagg cgggtgagca
     1621 gcgtggtgag ctgggcgcgg tcggccgggg accagtcggc cagtgccgcg tcgatcgtgc
     1681 aggcgcggag gtcgaacgcc tgctccaggg ccgctgcccc ctgctcggtc agcgtcagcc
     1741 ggtaggcgcg ccggtcgtcg gggtcgcagg tgcgcccgat caacccggcg tcttcgagac
     1801 tacgcacgtg ccggctcacg gtcgaggagt cgagcgagag cgcggccgcc acgccggaga
     1861 gccgcagcgg ctcggcccga caggccagcg tgtgcagcac cgcgagaccg gcccgctcga
     1921 ccggctcgtc gtactgcatg cgcttgagcc gaccggcgag agcgaagact gctgtcgcca
     1981 gctcgtcgcg ctgcggagtt gcttgcacca tgcaacttta cgccacgtcg gccggtggca
     2041 ggatgccgac atgagcatgg tccgcacgag caccgcatga gcaccagcat ccagccgtcg
     2101 ctgggcggtg ccgtcacgct cgacgacgtc ctggctgctc gtgcgctgct cgccgacatc
     2161 gcccgggtca ccccgatcga ggggtcccgc ccgctgagcg agcgggtcgg ggggccggtg
     2221 ttcctcaagt gcgagaacct ccaacgggcc ggctcattca agatccgcgg ggcctacacc
     2281 cggatcgccc ggctgtccgc cgacgagcgg gcccgtggcg tggttgcggc cagcgccggc
     2341 aaccacgccc agggggtcgc ccaggcggcc acgctgctcg gcacccgctc gacggtgttc
     2401 atgccggccg gtgccccgat ccccaaggtg gccgcgacca aggcctacgg cgccgaggtc
     2461 cgcttccacg ggcacaccgt cgacgacgcc ctgctggggg cggaggagtt cgcccgggcg
     2521 accggcgcgg tgctcatcca cccgttcgac cacccggacg tcatcgcggg gcagggcacg
     2581 atcgggctcg agatcctcga gcagtgcccc gacgtccgca cgatcgtcgc gggggtcggg
     2641 ggaggcgggc tcgtcgcggg gatcgccgtg gcggtcaagg ctctccgccc cgacgtacgc
     2701 gtcgtcgggg tgcaggccga gtcggccgcg gcctacctcc cgtcgctggc cgccggcaag
     2761 cccgtcgccc tcgacgcgat gtcgacgatg gccgacggca tcgcggtggg ctgcccgggc
     2821 cggctgccgt tcgcgatcgt gcaggagctg gttgacgacg tcgtcacggt ctccgaggag
     2881 ttcctctctc gcgccctgct gctcgtgctc gagcgcgcca agctggtcgt cgagccggcc
     2941 ggggcggcct cggtcgcggc cttgatcaac gcgccgacgg cctttgagcc gccggtggtc
     3001 gccgtgctct ccggcggcaa catcgacccg ttgctgctgc tgcgggtgct gcgtcacggg
     3061 ttggccgccg ccggccggtt cctgtcgctg cgggtgcggg tgtccgacca gcccggcgcg
     3121 ctggctcggc tgctcgccac gctggcccag gccgacgcga gcgtgctcga ggtcgagcac
     3181 gtgcgcaccg atcccaagct gagcctcgac gaggtcgagc tggccctgca gctggagacc
     3241 cgcggcatcg agcacagcga ggagctactg gccaccctgc gggcggccgg ctaccggctg
     3301 accttctcct gaccgtctcc tggcccgagc cgcgcccgct ccacgctggc tggcgacccg
     3361 gccgccggct acgccgagag cgcaacgacg cgccgggtaa atcccgtgct tactgtcaga
     3421 ggggtccggc atcctggcgc cgacgaccgt gatccgcccc caagggagtg ttccgatggc
     3481 cgacgatccg gcgatctatg ccgaagatct cgtcaagacc tacggcgagg tgcgagccct
     3541 cgacggcgtc gacctggccg tggcccaggg cacggtgctc ggcgtgctgg gccccaacgg
     3601 cgccggcaag acgaccgccg tacgcatcct caccacgctg ctgctgcccg actctgggcg
     3661 ggccagcgtc gcgggcttcg acgtggtccg tgaggcggct gcgctgcgtc aggtgatcgg
     3721 gctgtccggg cagtacgccg cggtcgacga gcacctgacc ggccgggaaa acctcgacat
     3781 ggtcggtcgg ctctaccacc tgggccgggc ggtctcccgc cagcgggccg gcgagctgct
     3841 cgaacgcttc gacctcgccg aggccgccga ccgacccgtc aagacctact ccggcggcat
     3901 gcgccgacgc ctcgacctgg ccggtgccct ggtcgccacc ccgcgggtgc tcttcctcga
     3961 cgagccgacc accgggctcg acccgcgcag ccggctgcag atgtgggacg tcatcgccga
     4021 gctcgtcgga ggcggtacga cgttgatgct caccacgcaa tacctcgagg aggccgaccg
     4081 gctggccgac cggatcgcgg tgatcgaccg gggcaaggtg atcgcccggg gcaccgccga
     4141 cgagctcaag acccaggtcg gcggcgagcg cctcgaggtg gtggttggcg ccgaccacga
     4201 cctggccaag gccacctcgg tcttggccga ggtgggccag gaggcgcccg ccgtcgagcc
     4261 gcacgctcga cggctcaccg tgccggtcat cggcggcacc ccggtgctga tcgaggcgtt
     4321 gcgccggctg cactccaacg gggtcgaggt gctcgacgtg gcgatccgcc ggcccaccct
     4381 tgacgacgtg ttcctcaccc tgaccggcca cgcggccgag gacgaggacg acgacaccac
     4441 gaccgccgcg gcggcaggcg gccgcggcag gaggcgagac aagtgagcca gctcgtgtcc
     4501 gcgctgggcg acgggctcgt cgtcaccaag cgcaacctca tcaagatcaa gcgggtgccc
     4561 gacctgctgg tgttcaccac gatgcagcca atcatgttcg tggtgctgtt cgcctacgtg
     4621 ttcggcgggg cgatcgccgt cgagggcgtc aactaccgcg agttcctgat ggccggcatc
     4681 ttcgcccaga ccgtgatctt cggtgcgacg ctcaccggcg ccggcctggc cgacgacatg
     4741 caaaagggcg tgatcgaccg gttccgctcg ctgccgatgt cccggtcggc cgtcctcgtc
     4801 gggcgcacgt tctccgacat gctcaacaac gtcatcgtca tcatcgtgat ggcggcgacc
     4861 gggctggtgg tgggctggcg catccacacc tcgttcctcg aggcgttggg cggcttcgcg
     4921 ctgctgctgc tgttctccta cgcgttctcc tgggtgatgg cctacgtcgg cctgatcgtg
     4981 cccagcgtcg aggtggtcaa caacgcggcg ttcatcgtgc tcttcccggt gaccttcatc
     5041 gccagcacgt tcgtgccgct cgcctcgctg ccgggcccgc tcgagaccgt ggcggcctgg
     5101 aacccggtca ccacgttgag cgagtcgggc cggctgctgt tcggcaacat cccggccgat
     5161 cccaacctgc gcaacctctc cgacgcctgg tcgatgcaga acccggtgtt ctacaccgtc
     5221 atctgcgccg cgctgatcct cgtggtcttc gtgccgctct cggtgcacaa gtaccgctcg
     5281 gccaccacca agtgacgcga gaaggggcgt cccggccgag ccgggacgcc ccttcttcgt
     5341 cgggtcagtg cgcctcagcc gctgtagggc ttggcgtcga ggatctccac cgtcacctgc
     5401 ttgccgttgg gcgcggtgta ggtggccgtg tcaccggtgt gcttaccgtt gatcgcctca
     5461 cccagcggtg agcgctcgga gtagacgtcg agctcaccgc cgctggcctc ccgagagccc
     5521 agcaggaagg tcatctcctg cccagccacc ttggcggtca ccaccatgcc gggctcgacc
     5581 accccgtcgt caggaggcgc ctggccgacc ttggcgtgct ggaggagggt ctgcagctgg
     5641 cggatgcgcg cctcctgctt gccctgctcc tccttggcag cgtggtagcc gccgttctca
     5701 cgcaggtcgc cttcttcccg ggcggcctcg atcctcttga cgatctctga gcgacccagg
     5761 ccggtgagct ggtccaactc ggccttcagc cggtcgtacg cctcctgggt caaccaggtg
     5821 acgctgctgg ttgcctcggt cacggattct cctgacgtcg tgcggaggtc ggcggcccct
     5881 cgccgccgcc ggtgttcggg ggtgaagaag acaggctaac aacactccgg gtgagcgcgc
     5941 ctctttccgc tcaggaaccg gccgcggggt cgcagccgag cacgagcccg gtcaccgccg
     6001 gcgcggtggt gcgcacgaca tcggcgcggg cgacggtggc ctcgccgccg gccggcacgt
     6061 caaccgtgag ccgcccgacc gagtcgtgga agcggtcctg tgcctccagc gtgcactgca
     6121 ccgcctccgc cgggtcgcgg gtcacctcgt agcgcacctg caccgcgtcg gggccggtca
     6181 cgctgtagcc gatcaggcgc gtggtggcgt cgggggcggc gtggtaggcg gccgaccaga
     6241 ggaaccagcc gagcgcgatg gcggccagca gcgcggcgcc catgacgagc agcatgtggc
     6301 gcagcgggtg agcatcctcg tcgtaccgtg ccatgggccg accgatcgtg tcggtccagc
     6361 tcagctgttc ttcggggatg agaggagcgc ccggttcgtc ggggacgggc gggtgctcgg
     6421 ggtcggtcac ggcggggcct ccggtgcggt gcgggctgga cgagggacac tggtgctcag
     6481 accatcacat gggtcgatcc gtggtccacc cgcggaccag cccgtgtgcc ggctcagacc
     6541 agcatggagc atcccaggtg accgagcgct tgcgcctcat ggccgtgcac gcccaccccg
     6601 acgacgagtc gagcaagggg gcggccacgg cggcccgcta cgtcgacgag ggcgtcgagg
     6661 tgctcgtcgt cacgtgcacc ggtggggagc gcggcagcat cctcaacccg cggctggccc
     6721 acgaccccgc ggtgctggcc aatctggccg aggtccgccg cagcgagatg gacgccgccc
     6781 gggcgatcct cggcgtcgag caggcctggc tggggttcgt cgactcgggg ctgcccgagg
     6841 gcgacccgct cccgccgttg cccgagggct gcttcgcgct gaccccgctc gaggtggccg
     6901 ccgagccctt ggtgcggctg gtccgtgagt tccgcccgca cgtgatgacg acgtacgacg
     6961 agaacggcgg ctacccgcac cccgatcacg tcatgtgcca ccgggtctcc gtggaggcct
     7021 accacgccgc cgccgacccg gcccgctacc ccggcaccgg ggacccgtgg gcgacgccca
     7081 agctctacta catgcagacc atgtccctcg cgcgcacgct ggctctgcac gagagcatgc
     7141 tcgcggccgg gcttgactcg cccttcgcgg aacgcatcga gcgctaccgc ggccgagagc
     7201 cgcgcaccat caccacgcgg gtgccgtgcg ccgactggtt cccggtgcgc gaccgggccc
     7261 tgctggcgca cgccacccag gtcgaccccg acggggcctg gttcgcctgc ccgatggaca
     7321 tccagcgcga cgtgtggccg accgaggact acgagctggc ccactcccgg gtggccaccg
     7381 acctgcccga ggacgacctg ttcgccggcg tgcgcgagct cgtgccgggc acggtcaccg
     7441 gccccgacgc cgacctcgac accgacttcg acaccgacct cgacaccgac ctcgacaccg
     7501 acaaggagct ggcatgagcg ccatggtgac tctcggactc gcgccgaccg gtgccgccgt
     7561 ggccgtgccg accgcggtgc tcgccgacgt cgacccggcc aaggtgcaga ccggggcgat
     7621 cggcatggtg gtcttcctgc tgctgtgcgg ggcgaccgcc ctcctgctgc ggtccttccg
     7681 caaccagatg cgcaaggtcg agcaggccga cctgccccac gagaagcccc gcccgcacgg
     7741 cccccggccg ggcatccgcc tgcccagcgc cgagcccgag cccgggtcgt cgcccgagcc
     7801 tcaacccggc tcgcagcccg gccccaacgg cgagcacccc cgcggctagc ccgcccggga
     7861 cctcggtccc tggtcgtgat cccctccgcg tgctcgactg acggagtcgg agctagaccc
     7921 gcaggggaca tgcaggggac atggaggtgg acgatgcctc ggacatcgca gtcagtgacg
     7981 cggcgtcggc gcccagaggc ctcgctgtgg tcggagttca tgggctggcc ggagtcggtg
     8041 ccgttcggcg agctcttccg ggcgatgcgt ggtgagggca tccgggtcga ggagtacgtc
     8101 gacgacggca ccctcgtcat ccgcgccgat ctgcccggca tcgatcccga gaaggacgtc
     8161 gacgtctcgg tgagcaacgg gatgctgcgg atcaaggctg agcgccagga gtcgaagaag
     8221 ggcgagaccg cctccggtta tcgctctgag gtccactacg gcagcctcag ccgggtgatg
     8281 ccgctgccgg gcggggtgac cgagaaggac gtcaccgcca cctatagcga cggcgtgctt
     8341 gagatccggc tgccgatggc cacggaagag gagaccacgc acagcatccc gatccagcgc
     8401 ggctgaggcg cccggtcacc ggtggggcag agtggggcca tgcccaaccg cctggccact
     8461 gcgacctcgc cctacctgct gcagcacaag gacaacccgg tcgactggtg gccctggtcc
     8521 gacgaggcct gggcggaggc cgtccgccgt gacgtgccgg tgttcctctc ggtcggctac
     8581 gccgcctgcc actggtgcca cgtcatggcg cacgagtcgt tcgaggaccc ggaggtggcg
     8641 gcgttcctcg ccgagcactt cgtcagcatc aaggtcgacc gcgaggagcg tcccgacgtc
     8701 gacgcggtct acatgcaggc cacccaggcg ctgaccgggc agggcggctg gccgatgacg
     8761 gtcttcgcca gcccggcagg tgagcccttc tacgcgggca cctacttccc accccggccg
     8821 gcgcacgggc tgccgtcgtt ccgccagctc atcgaggcga tcgcggcctc gtggcgtgag
     8881 cgccgggccg aggtggtctc ggccagcgag tcgatcaccg cgcagctggc cgccgtacgt
     8941 cctgccgggt ccggacagac tcccctgacc gccgagctgc tggacaccgc ggtggccgcg
     9001 ctggcccggg agtacgacgc gacgcacggc ggcttcgggg gcgcgccgaa gttcccgccg
     9061 tcgatggtgc tggagttcct gctgcgccag cacgcccgca ccggcgcatc cgagtcgctg
     9121 gccatggtcg ccggcacctg cgaggcgatg gcccgcggcg gggtctacga ccagctcggc
     9181 ggcgggttcg cccgctacgg cgtggaccgg gcctgggtgg tgccgcactt cgagaagatg
     9241 ctttacgaca acgcgctgct gctgcgggtc tacctgcact ggtggcgggc caccggcgcg
     9301 tcgctggccg agcgggtggt ccgcgagacc gccgagttcc tgctgcgcga cctgcgcacc
     9361 cccgagggcg ggttcgccgc gtcgctggac gccgacacgt tggtcgagac ggcgcacggc
     9421 ccgcgcgggg tcgagggcgc cacctacgtc tggacccccg agcagctgcg cgcggtgctc
     9481 ggcgacgacg acggcgcctg ggcagcggcc ctgctcgaag tgacggacgc cggcacgttc
     9541 gagcacggcg cgtccacgct gcagctgttg cgcgaccccg acgactcggc ccgctgggac
     9601 gtcgtacggc gccggctgct cgccgcccgg gccgcccgac cgcagccgag ccgtgacgac
     9661 aaggtggtcg ccgcctggaa cgggctggcg atcgcggcgc tggccgaggc cggcgcgctg
     9721 ctcgacgaac cggcctacgt cgaggccgcc gtggccgccg ccgacctgct ggtcacggtg
     9781 cacctggacg agcggggccg gctgcggcgc acctcgcgcg atggccgggt cggtgactcg
     9841 gccgggctgc tggaggacca cgccgacgtg gccgaggggc tgctcgcgct gcaccaggtg
     9901 accggcgacc cggtctggct ctcgttggcc gacgggctgc tcgcggtggt gctggccgac
     9961 ttcgccgacg ccgatgggct gttgtacgac accgccctcg agcgcaccga cccccgggtg
    10021 ctgcggctgg gccgacccgc cgaccccacc gacaacgcca ccccctcggg gc
//
LOCUS       DATIBB010000158         4818 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_1016957, whole genome shotgun sequence.
ACCESSION   DATIBB010000158 DATIBB010000000
VERSION     DATIBB010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 4818)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4818)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4818
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1016957"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..173)
                     /locus_tag="VLC50_06025"
     CDS             complement(<1..173)
                     /locus_tag="VLC50_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019545427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HSF27059.1"
                     /translation="MSGTRIVVVGGGPGGYEAALVAAQLGAEVTLVHGEAIGGSAVLT
                     DAVPSKTLIATAEA"
     gene            246..698
                     /locus_tag="VLC50_06030"
     CDS             246..698
                     /locus_tag="VLC50_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012854491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HSF27060.1"
                     /translation="MALYAAYGSNADPHQMAQRAPHSPPSGTGWLVGWRLTFGGEDRG
                     WEGALATVVEQADAAVFVMLYDLTPEDERALDRWEGAEIGLYSKLRVRVQTLDGDALA
                     WLYVLDGYEGGLPSARYLGILADAVEAAGAPDDYVRELRARACRSLGE"
     gene            766..1581
                     /locus_tag="VLC50_06035"
     CDS             766..1581
                     /locus_tag="VLC50_06035"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HSF27061.1"
                     /translation="MSEGPDDPGDPYDAARVAAAALAERTGIARHDVAVVLGSGWVPA
                     ADQLGQTVTELAVTDLPGFAPPAVEGHAGRIRSVRTAHGHALVFLGRTHLYEGHGVAA
                     VVHGVRTAVAAGAQVVVLTNGCGGLRPHWTPGTPVLISDHVNLTGTSPIVGANFIDLT
                     DLYSPRLRALCREVEPGLEEGVYVQFRGPHYETPAEIRMVAAIGGDLAGMSTALEAIA
                     VREAGAEVLGLSLVTNPAAGISGAPLDHADVLAAGAASAERMGRLLAAVLDRL"
     gene            1606..2409
                     /locus_tag="VLC50_06040"
     CDS             1606..2409
                     /locus_tag="VLC50_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012801768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HSF27062.1"
                     /translation="MHVAVTGAAGEIGSTLLPGLRALGHDVRGLDRRPPDAPDPQVQL
                     GDVRDAAALDELLRGADAVVHLAGNPNETDLPTALESHVLSTAAVLEAMVRNKVGRLV
                     LASSNHAVGRTPRSNLLRTDVAHRPDTFYGVGKVAAEALTHLYVDRHGLSAVCLRIGS
                     FLDRPTSRRALSTWLSPADAVRLVHAALTAADVGFAVVYGVSANTRGWWDLGPARALG
                     YEPRDDAETYADEVLATPASDEDENEAAYVGGPYATDTFARPAFDEVPA"
     gene            2406..4100
                     /locus_tag="VLC50_06045"
     CDS             2406..4100
                     /locus_tag="VLC50_06045"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018561311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HSF27063.1"
                     /translation="MSGGPDPAQLLEAAHAWLAEDPDPDTRAELAALLETAESDDAAA
                     AKAALAELADRFVGPLQFGTAGLRGALGAGPNRMNRAVVIRAAAGLGAYLREHGGDAI
                     VIGYDARHKSDVFAADTAAVMTGLGIRAVVLPRPLPTPVLAFAIRHLGVDAGVMVTAS
                     HNPPQDNGYKVYLGDGSQIVPPTDAEISARIAAVGPLAEVFLPDNGWDTLTDADVLEP
                     YLDRVAALVRQDAPRDLRIVYTPLHGVGGEVAHAAFLRAGFRAPAVVPLQALPDPDFP
                     TVAFPNPEEPGALDLAFAAARESAADLVIANDPDADRCAVAVPDPQVPGGWRMLRGDE
                     LGALLGQHVARRGVPAGAVFATSIVSSSLLGKIAAAAGIGYTETLTGFKWISRVPGLA
                     FGYEEALGYCVDPDGVRDKDGISAALLIAELAATLKAEGRSLRDVLDELAREHGLHAT
                     DQLAVRVSDPSLIDEAVRRLRAHPPTTLAGQAVESIEDLATGVDGLPPTEALRCRLAD
                     AARVVVRPSGTEPKIKCYLEVVEDVADGNVSAARARAAAELARLKADVAAAAGLPV"
     gene            4125..>4818
                     /locus_tag="VLC50_06050"
     CDS             4125..>4818
                     /locus_tag="VLC50_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005455630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HSF27064.1"
                     /translation="MTSGRVRVAVLDDYQGVALSLADWSPVLEQADVDAFREHLPDAD
                     ALVRALAPYDVIVLMRERTPMPRPVIEALPRLRLLVTTGARNASIDLAACADRGVTVC
                     GTPGSGAATAELTWALLLACARRLDVELASLRSGGWTAGLGTELRGRTLGVLGLGRLG
                     SAVATVGLAFGMRVLAWSQNLTPERCAEVGVEHASLDTLLGESDFVSVHLKLSSRTRG
                     LLGEAALRSMKPT"
BASE COUNT          601 a         1821 c         1756 g          640 t
ORIGIN      
        1 gcctcggcgg tggcgatcag cgtcttgctg ggcacggcat cggtgagtac ggcgctgccg
       61 ccgatcgcct cgccgtggac cagggtgacc tcggcaccga gctgcgcggc caccagcgcc
      121 gcttcgtaac cgcccgggcc accaccaacg acgacgatgc gcgtgccact caccgggaca
      181 ttcaaccgga accaaccgtg ccgacgatca gcggatgtcg ccgtactgcc ctctaggctc
      241 ctgccatggc cctctacgcc gcctacggga gcaacgccga cccgcatcag atggcgcagc
      301 gagccccgca ctccccgccg tcgggcaccg ggtggctggt tggctggcgg ctgaccttcg
      361 gcggcgaaga ccgcggctgg gaaggcgcgc tggccaccgt ggtcgaacag gccgacgcgg
      421 cggttttcgt catgctctat gacctcacgc ccgaggacga gcgggcgctt gaccgctggg
      481 agggcgccga gatcgggctc tactccaagc tgcgggtgcg cgtgcagacc ctcgacggcg
      541 acgcgctggc ctggctctac gtgctcgacg gctacgaggg cgggctgccg tcggcccgct
      601 acctgggcat cctcgccgac gcggtcgagg ccgccggcgc gcccgacgac tacgtgcgcg
      661 agctgcgggc ccgcgcctgc cgctccctcg gcgagtgacc cgcgcagcac tggtcgcgat
      721 caagcaatct ctgcacacct aggggcgact agggtcgagg ccgtgatcag cgaaggtccc
      781 gacgaccccg gcgacccgta cgacgcagcc cgcgtggccg cggccgcgct ggccgagcgc
      841 accgggatcg cccgtcacga cgtcgccgtg gtgctcggct caggctgggt gccggccgcc
      901 gaccagctcg ggcagacagt caccgagctg gcggtcaccg acctgcctgg cttcgccccg
      961 ccggcggttg agggccacgc cgggcggatc aggtcagtgc gtacggcgca cgggcatgcg
     1021 ctggtcttcc tcggccgcac ccacctctac gaaggtcacg gcgtcgccgc ggtggtccac
     1081 ggcgtgcgca ccgcggtggc ggccggggcg caggtcgtcg tactcaccaa tggctgcggg
     1141 gggctgcgcc cgcactggac cccgggcacc ccggtgctga tcagcgacca cgtcaacctc
     1201 accggcacct cgccgatcgt cggggccaac ttcatcgacc tgaccgacct ctactcgccg
     1261 cggctgcggg cgctgtgccg cgaggtcgag cccgggctcg aggagggcgt ctacgtccag
     1321 ttccgcgggc cgcactacga gaccccggcc gagatccgca tggtcgccgc gatcggcggc
     1381 gacctcgccg gcatgtccac cgcgctcgag gcgatcgcgg tccgggaggc cggcgccgag
     1441 gtgctcggcc tgtcgctggt gaccaatccg gccgccggca tctcgggggc gccgctcgac
     1501 cacgccgacg tgctcgccgc cggcgcggcc tcggccgagc ggatgggccg cctgctcgcc
     1561 gccgtactcg accggctctg aacgcccagt aagaggagat cgctcatgca cgtcgccgtc
     1621 accggggccg ccggcgagat cggctcgacg ctgctgcccg gactgcgggc tctcgggcac
     1681 gacgtgcgcg ggctcgaccg acgcccgccg gatgcgcccg acccgcaggt ccagctcggc
     1741 gacgtgcgcg acgccgcggc gctcgatgag ctgctccgcg gtgccgacgc ggtggtgcac
     1801 ctggccggca accccaacga gaccgacctg ccgaccgcgc tcgagtcgca cgtgctcagc
     1861 accgccgcgg tgctggaggc catggtgcgc aacaaggttg gccgcctggt gctggccagc
     1921 agcaaccacg cggtcggacg cacgccccgc agcaacctgc tgcggaccga cgtggcgcac
     1981 cggccggaca cgttctacgg cgtcggcaag gtggccgccg aggctctgac ccatctgtac
     2041 gtcgaccggc acggcctgtc cgcggtctgc ctgcggatcg gttcgttcct cgaccgcccg
     2101 accagccgtc gggcgctgtc gacctggctg tcgccggccg acgccgtacg cctggtgcac
     2161 gcggcgctga ccgccgccga cgtcgggttc gccgtcgtct acggggtctc ggccaacacc
     2221 cgcggctggt gggacctcgg gccggccagg gccctgggtt atgagccgcg cgacgacgcc
     2281 gagacctacg ccgacgaggt gctcgccacc ccggcgagtg acgaggacga aaacgaggcg
     2341 gcgtacgtcg gcgggcccta tgccaccgac acgtttgccc ggccggcctt cgacgaggtg
     2401 ccagcatgag cgggggcccg gaccccgcgc agctgctcga agcggcgcac gcctggctgg
     2461 ccgaggatcc cgaccccgac acccgggccg agctcgccgc gctgctcgag acggccgagt
     2521 cagacgacgc ggcggccgca aaggccgccc ttgcggagct ggccgaccgg ttcgtcggcc
     2581 cgctgcagtt cggcaccgcc gggctgcgcg gggcgctggg cgccgggccc aaccggatga
     2641 accgcgcggt ggtgatccgg gccgcggccg ggctgggcgc ctacctgcgc gagcacggcg
     2701 gcgacgcgat cgtgatcggc tacgacgccc ggcacaagtc cgacgtgttc gccgccgaca
     2761 ccgccgcggt gatgaccggg ctgggcatcc gggccgtcgt acttccccgg ccgctgccca
     2821 ccccggtgct ggccttcgcg atccgccacc tcggggtgga cgccggggtg atggtcaccg
     2881 cctcgcacaa cccgccgcag gacaacggct acaaggtcta tctcggtgac ggctcgcaga
     2941 tcgtgccacc caccgacgcc gagatctccg cgcggatcgc cgcggtcggc ccgcttgccg
     3001 aggtgttcct cccggacaac ggctgggaca ccctcaccga cgccgacgtg ctcgagccct
     3061 acctcgaccg ggtcgccgcg ctggtgcgcc aggatgcccc gcgcgacctg cgcatcgtct
     3121 acaccccgtt gcacggcgtc ggcggcgagg tcgcgcacgc ggcattcctg cgcgccggct
     3181 tccgcgcccc ggccgtcgta cccctgcagg ccctgcccga ccccgacttc ccgacggtgg
     3241 ccttccccaa cccggaggag cccggcgccc tcgacctggc cttcgccgcc gcgcgcgagt
     3301 cggcggccga cctggtcatc gccaacgacc ccgacgccga ccggtgcgcg gtcgccgtac
     3361 ccgatcctca ggtgcccggc ggctggcgga tgctgcgcgg tgacgagctc ggcgcgctgc
     3421 tcggccagca cgtcgcccgg cgcggggtgc cggccggcgc ggtgttcgcc acctcgatcg
     3481 tctcgtcgtc gctgctcggc aagatcgccg cggcggccgg catcggctac accgagacgt
     3541 tgaccgggtt caagtggatc tcgcgggtgc ccgggctggc gttcggttat gaggaggcgc
     3601 tgggctactg cgtcgacccc gacggcgtgc gcgacaagga cggcatctcg gcggcgctgc
     3661 tgatcgccga gctggccgcc acgctcaagg ccgagggccg gtcgctgcgc gacgtgctcg
     3721 acgagctggc ccgcgagcac ggcctgcacg cgaccgacca gctcgcggtg cgggtcagcg
     3781 acccgtcgct gatcgatgag gcggtacggc ggttgcgcgc gcaccctccg accacgctgg
     3841 ccgggcaggc ggtcgagtcg atcgaggacc tcgccaccgg cgtcgacggg ctgccgccga
     3901 ccgaggcgct gcggtgccgg ctggccgacg cggctcgggt cgtcgtacgt ccgtcaggca
     3961 ccgagccgaa gatcaagtgc tatctcgagg tggtcgagga cgtggccgac gggaatgtct
     4021 ccgcggcccg ggcccgcgcc gccgccgagc tggcccggct gaaggccgac gtggccgcgg
     4081 ccgccggact gccggtctga ccggtggctg aaggagtgtc gacggtgacg agtgggcggg
     4141 tgcgggtcgc ggtgctcgac gactaccagg gcgtcgcgct ctcccttgcc gactggagcc
     4201 cggtgctcga gcaggccgac gtcgacgcgt tccgcgagca cctgcccgac gcggacgcac
     4261 tggtgcgggc gctagcgccg tacgacgtca tcgtgctcat gcgcgagcgc accccgatgc
     4321 cgcggccggt gatcgaggcg ctgccccggc tgcggctgct cgtcaccacc ggcgcgcgca
     4381 acgcgtcgat cgacctggcc gcctgcgccg accggggcgt cacggtgtgc ggcacccccg
     4441 gctcgggtgc cgccacggcc gagctcacct gggcgctgct gctggcctgc gcgcgccggc
     4501 tcgacgtcga gctggccagc ctgcgctccg gcggctggac cgcgggactg ggcaccgagc
     4561 tgcgggggcg caccctggga gtgctcggcc tcgggcggct cggctcggcg gtggccaccg
     4621 tcgggctggc cttcgggatg cgggtgctgg cctggagcca gaacctcacg ccggagcgct
     4681 gcgccgaggt cggggtcgag cacgcctcgc tggacacgct gctcggcgag tccgacttcg
     4741 tctcggtgca cctcaagctc tcctcgcgca cgcggggcct gctcggcgag gcggcgctgc
     4801 ggtcgatgaa gccgaccg
//
LOCUS       DATIBB010000159         7728 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_1676806, whole genome shotgun sequence.
ACCESSION   DATIBB010000159 DATIBB010000000
VERSION     DATIBB010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 7728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7728
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1676806"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(104..910)
                     /locus_tag="VLC50_06055"
     CDS             complement(104..910)
                     /locus_tag="VLC50_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HSF27065.1"
                     /translation="MSQARSTDRHGASCVRLAVANQKGGVAKTTTVVNLGAALAEAGA
                     RVLVVDLDAQSCLTFALGVDPETVEVTMGEVIVDGAPVAEAVVGTGEGLDLLPGSIEL
                     AAAEAALLAMPDGRCVLRETLEPLAAAYDVLLLDCAPSLGMLTLGALAAADEVLVPLQ
                     CEMLAHRGVGQLLDTVADVTRLLNPRLRVRGLLPTMYDGRSNHARAVLADLPGRYRQP
                     VLDPIPRSIRFAEAAALGRSVLRTARGARAAHAYRALAADLLADWAGQQT"
     gene            954..1859
                     /locus_tag="VLC50_06060"
     CDS             954..1859
                     /locus_tag="VLC50_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018834995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HSF27066.1"
                     /translation="MSTPPFLDLPLCARARRITTERGELATLVAECAPAPGSTEVAMG
                     TAVLVPGFTGSKEDFIGVLEPLATRGWRVVALDQRGQFESTPDPDSGPAGGDDPGRYD
                     VEELAADLAALISALGTGPVHLLGHSFGGLVARAATIASPGLLRSLTLLGSGPGPVPA
                     ASQARLALLMQALASLDLATIWEAMQALDAQAGVVPPPEPVLEFLRGRFLANSPTGLA
                     RLAHQLLTCPDRTDELAAATARGGLPVLVAHGADDDVWTPHELAAVARRLGAAYEVVD
                     GAGHSPAADRPLATAAVLDRFWRGA"
     gene            complement(1862..2158)
                     /locus_tag="VLC50_06065"
     CDS             complement(1862..2158)
                     /locus_tag="VLC50_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012725577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HSF27067.1"
                     /translation="MIFKAVGDGRPYPDHGHVTAKQWSDVPPRQVRLDELITTKRSLD
                     LDTLLDEDSTFYGDLFAHVVQWRGDLYLEDGLHRALRAALHQRLMIHARVLVVT"
     gene            complement(2155..3087)
                     /locus_tag="VLC50_06070"
     CDS             complement(2155..3087)
                     /locus_tag="VLC50_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012851564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HSF27068.1"
                     /translation="MSAVAASEPVEPAPAPAPAGAPARLFSCTPTRLTTWVDCPRRYR
                     FTYLQRPTPPKGPPWAHNSFGASVHAALARWWSLPLPRRTAAAGGDLLVAGWLTDGYR
                     DEAQSTRWRDRARAMVTGYLGGIDPSAEPAGVERTVAARTPRLALSGRVDRIDRCDTP
                     AGEQLVIVDYKTGRWVPDTHDVRGSLALAVYAVAASRVLRAPARRVALHHLPSGQVVE
                     HEHTDVALARHLDRAERLGAEAAAAEQGWRTGLAEAPAAAADERFPPRPGPLCGWCDF
                     RRHCPQGRAAAPDREPWAGLADDPASEATTRLGE"
     gene            complement(3084..3695)
                     /locus_tag="VLC50_06075"
     CDS             complement(3084..3695)
                     /locus_tag="VLC50_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012877552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HSF27069.1"
                     /translation="MSGIDWKLFVEVFVTLFVIVDPVGTVPLFLSLTSGRTRKARHRL
                     ATQAVLVSFLVITVFAVFGQSILHYLGITLPALQAAGGLLLLLVALELLTGKADEPTE
                     TKNVNVALVPLGTPLLAGPGAIAATIVFVRRADDWSDAVMLALGIIAVHLVLWLSMRY
                     SVGLIKLIKDSGVTLVTRISGLLLAAIAVQLFADAVTVFVTSA"
     gene            complement(3692..4531)
                     /locus_tag="VLC50_06080"
     CDS             complement(3692..4531)
                     /locus_tag="VLC50_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018156713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HSF27070.1"
                     /translation="MRIDLHTHSAVSDGTEAPAELMAAAVAAGLDAVALTDHDTVLGW
                     AEAAAAATRLGVCLVPGAELTCADGPDSLHLLAYLFDPDHPALAREMALSRDDRVPRA
                     EEMVRRLAADGFPVTMAAVRAQTTDATTLGRPHIADALVAAGVFASRDQAFATVLRDD
                     SPYYVGHHSTPAAVAVRLVREAGGIPVLAHPRSHRVVTDAVIAGLTDAGLLGLEVDHP
                     DHDPEARAALRALAADLGLLVTGSSDYHGTGKSTPIGAETTDPDVYTALLAQATGPTT
                     VVA"
     gene            4608..5009
                     /locus_tag="VLC50_06085"
     CDS             4608..5009
                     /locus_tag="VLC50_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019875709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HSF27071.1"
                     /translation="MHVPCVGAVVHDDDGRLLVVQRGHDPAMGRWSIPGGRVEAGETD
                     VQAVAREVLEETGLHVTVGVRLGTVQLPGPDGVVYDVRDYACAIAVPGTTPVAGNDAA
                     AVRWVSRVELTHLDLTDGLVDTLTQWGALPH"
     gene            complement(5099..5485)
                     /locus_tag="VLC50_06090"
     CDS             complement(5099..5485)
                     /locus_tag="VLC50_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015357617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HSF27072.1"
                     /translation="MRINLASVLVDDQDKALRFYTEVLGFVKKTEIPLGDARWLTVVS
                     PEDPDGVELVLEPDSHPAVRPFKDALAADGIPFTSFAVADVPAEAERLKAQGVRFTQE
                     PTQMGPVTTAVFDDTCGNLIQIASQP"
     gene            complement(5482..5769)
                     /locus_tag="VLC50_06095"
     CDS             complement(5482..5769)
                     /locus_tag="VLC50_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011209366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HSF27073.1"
                     /translation="MADVFKALADSTRRAILDELQERDGQTLFELCGRLAMKHGLSSS
                     RQAISQHIAVLEDAGLVATHREGRYTFHRLDKTPLRTITDRWPIDPERKPR"
     gene            complement(5803..6936)
                     /locus_tag="VLC50_06100"
     CDS             complement(5803..6936)
                     /locus_tag="VLC50_06100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF27074.1"
                     /translation="MNADVETQLRATLEEQAGVMERMDVGLLADRVMARGRARRRRRA
                     IGAAAAAVFVVVALVAVPAWVSGTHAPVRPTMATQLPEPSVTGRSAVPSHRAIASYRQ
                     VLAWFQGLPEAQGPVSFTGDPWGVLEIVPGDTSNDADESHTVTRLRATGQVLMTGKPR
                     GAALDSGRHRVAVAAAVPDPKAGPSLTITLMVFDADTGRMLSRVSGLDSGTRVSGWLD
                     GRVVLDVYASNWANRVAWWDPDAPEAAPVDITAGRVLDVAESGHRILVEGPGARCSQV
                     WEVIGKPHQALEECGMRLVALSDDGRRGFIDGRYGIAPAMLDVWSGAQVALKLPGPVS
                     VASARWSADDRVVLSLTVWGLKDGVTVVCEPDDGSCARVVPSP"
     gene            complement(6933..7499)
                     /locus_tag="VLC50_06105"
     CDS             complement(6933..7499)
                     /locus_tag="VLC50_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020578647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SigE family RNA polymerase sigma factor"
                     /protein_id="HSF27075.1"
                     /translation="MVGEAGESGAGGGRDAEFRAYVVARSHLLLRTAYLVTGDHQLAE
                     DLLQTALTKTYLAWGRIRDRESIDAYVRRVMVTTHVSWWRRKWRAETPIGEVPDGLAA
                     AAAGIDESADAVERLAMWALLRGLPPRQRAVVVLRYYEDLSETEIAQTLGCTPGTVKS
                     QAHRALASLRNALQAADAPVVEPSGGER"
     gene            complement(7612..>7728)
                     /locus_tag="VLC50_06110"
     CDS             complement(7612..>7728)
                     /locus_tag="VLC50_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020462001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HSF27076.1"
                     /translation="GCNQDGTLVCIFRRKVMVPKQSYLDARGGEQPGRPSLH"
BASE COUNT         1088 a         2822 c         2774 g         1044 t
ORIGIN      
        1 cccgcgcgcg ggcgccccac ggcgccggcg tcgccgtcgc cccactggcg agacggccgc
       61 cggggcgatc aacccggccg aggccagcgc cgcgtcctga cgctcaggtc tgctggccgg
      121 cccagtcggc cagcaggtcg gcagccaacg cccggtaggc gtgggccgcg cgcgcgccgc
      181 gcgcggtccg cagcaccgag cgccccagcg cggccgcctc ggcgaagcgg atcgagcggg
      241 ggatcgggtc gagcaccggc tgcctatagc gaccgggcag gtcggcgagc accgcacgcg
      301 cgtggttgct gcgcccgtcg tacatcgtgg gcagcagccc acgcacccgc agcctggggt
      361 tgagcagccg cgtcacgtcg gcgaccgtgt cgagcagctg gccgacgccg cggtgcgcga
      421 gcatctcgca ctgcagcggt accagcacct cgtcggccgc ggccagggcg ccgagcgtga
      481 gcatgccgag cgacggagca cagtcgagca gcaggacgtc gtacgccgcc gccagcggct
      541 ccaacgtctc gcgcagcacg caccgaccgt cgggcatggc cagcagcgcg gcctctgcgg
      601 cggccagctc gatcgagccc ggcagcaggt cgaggccctc gcccgtgccg accacggcct
      661 cggccaccgg ggcgccgtcg acgatcacct cgcccatcgt gacctcgacc gtctccgggt
      721 cgacccccag cgcaaacgtc aggcacgact gggcgtcgag gtcgacgacc agcacccgcg
      781 ccccggcctc ggccagggcg gcgccgaggt tgaccaccgt ggtggtcttg gccaccccgc
      841 ccttctggtt ggccaccgcc agccgcacgc acgaggcgcc gtggcggtcg gtgctgcggg
      901 cctggctcac gaggggccat tctgcccagg ccgccccggt aacgtgcccg atcgtgagca
      961 cgccaccgtt cctcgacctg ccgctgtgcg cgcgggcccg acgcatcacg accgagcgcg
     1021 gcgagctggc cacgctcgtc gccgagtgcg ctccggcccc cggcagcacc gaggtggcga
     1081 tgggcaccgc ggtgctggtg cccgggttca ccggctccaa ggaggacttc atcggcgtcc
     1141 tcgagccgct ggccacccgg ggatggcgcg tggtggccct cgaccagcgc ggccagttcg
     1201 agtccacgcc cgatcccgac tcggggccgg cggggggcga tgacccgggg cggtacgacg
     1261 tcgaggagct ggccgccgac ctggcggcgc tgatcagcgc cctgggcacc gggccggtgc
     1321 acctgctggg ccactcgttc ggcgggctcg tggctcgggc cgccacgatc gcgtcgcccg
     1381 gcctgctgcg ctcgctgacc ctgctgggca gcgggccagg gccggtcccg gcggcaagtc
     1441 aggctcggct ggcgctgctg atgcaggcgt tggccagcct cgacctggcg acgatctggg
     1501 aggcgatgca ggcgttggac gcgcaggccg gggtggtgcc gccgcccgag ccggtgctcg
     1561 agttcctgcg cgggcgcttc ctcgccaact ccccgaccgg gctggcccgc ctcgcccacc
     1621 agctgctcac ctgcccggac cgcaccgacg agctcgccgc ggccactgcc cggggcggcc
     1681 tgccggtgct ggtggcccac ggtgccgacg acgacgtctg gaccccgcac gagctggccg
     1741 cggtcgcgcg ccggctcggt gcggcctacg aggtggtcga cggggcgggg cactcaccgg
     1801 cggccgatcg accgctggcc accgccgcgg tgctcgacag gttctggcgc ggcgcctgac
     1861 ctcaggtcac gacgagcacg cgcgcgtgga tcatcagccg ctggtgcagc gcggcgcgca
     1921 gggcccgatg caggccatct tcgagataga ggtcgccccg ccactgcacg acatgggcga
     1981 acaggtcgcc gtagaacgtc gagtcctcgt cgagcagcgt gtcgaggtcg agggagcgct
     2041 tggtggtgat cagctcgtcg aggcgcacct gccgcggcgg gacgtccgac cactgcttgg
     2101 ccgtgacgtg gccgtggtcg ggatagggcc ggccgtcgcc gaccgccttg aagatcactc
     2161 ccccagtcta gttgtcgcct ccgacgccgg gtcgtccgca agtccggccc acggctcgcg
     2221 gtcgggcgcg gcagcccggc cctgcgggca gtgccggcgg aagtcgcacc agccgcacag
     2281 cggcccgggt cggggtggga accgttcgtc ggcggccgcc gcgggagcct cggccagccc
     2341 ggtgcgccag ccctgctcgg ccgcggccgc ctcggcgccg agccgctcgg cgcggtccag
     2401 gtggcgggcc agcgcgacgt cggtgtgctc gtgctcgacg acctgccccg aaggcaggtg
     2461 gtgcagcgcc acccggcgcg ccggcgcgcg cagcactcgg gaagctgcca ccgcgtagac
     2521 ggccagggcc agcgagccgc gcacgtcgtg ggtgtcgggg acccagcggc cggtcttgta
     2581 gtcgacgatc accagctgct caccggccgg cgtgtcgcac cggtcgatgc ggtcgacccg
     2641 ccctgacagc gccagccgcg gggtgcgggc cgcgacggtg cgctcgacgc cggccggctc
     2701 ggcgctcggg tcgatcccgc ccaggtagcc ggtcaccatg gcccgggccc ggtcgcgcca
     2761 ccgcgtcgac tgggcctcgt cgcggtaccc gtcggtgagc cagccggcga ccagcaggtc
     2821 tccacccgcg gcagcggtgc gccggggcag cggcaggctc caccatcggg ccagcgcggc
     2881 gtgcacgctg gccccaaagc tgttgtgcgc ccacggcggt cccttgggcg gggtcggacg
     2941 ctgcaggtag gtgaaccggt agcggcgcgg gcagtcgacc caggtggtca gccgggtcgg
     3001 cgtgcaggag aacaaccgag ccggggcgcc ggctggggcg ggcgcaggag ccggctcgac
     3061 cggctcgctc gccgcgaccg cgctcatgcg ctggtgacga acacggtgac ggcatcggca
     3121 aacagctgaa cggcgatggc ggccagcagc agcccggaga tgcgggtcac cagcgtgacc
     3181 ccgctgtcct tgatgagctt gatcaagccc acggagtagc gcatcgacag ccacagcacg
     3241 aggtgcacgg cgatgatgcc cagcgccagc atcacggcgt cggaccagtc atcggcccgt
     3301 cgtacgaaca cgatcgtcgc cgcgatcgcg ccggggccgg ctagcagcgg ggtgcccagc
     3361 ggcaccaggg ccacgttgac atttttggtc tcggtcggct cgtcggcctt gccggtgagc
     3421 agctcgagag ccacaaggag gagcagcagc ccgccggccg cctgcagcgc cggcagcgtg
     3481 atgccgaggt agtgcaggat gctctgcccg aacaccgcga agaccgtgat gacgaggaac
     3541 gacaccagga ccgcctgggt ggccaaccga tggcgggcct tgcgggtgcg gccggaggtg
     3601 aggctgagga acagcggaac cgtgccgacg gggtcgacga tgacgaacag cgtgacgaag
     3661 acctcgacga agagcttcca gtcgatcccg ctcacgcgac gaccgtggtc ggtccggtgg
     3721 cctgggccag cagcgcggtg tagacatcgg gatcggtggt ctcggcgccg ataggagtcg
     3781 acttgccggt gccgtggtag tcgctggacc cggtgaccag caaccccaga tcggcggcca
     3841 gcgcgcgcag ggcggcccgg gcctccgggt cgtgatcggg gtggtcgacc tccagaccga
     3901 gcagcccggc gtcggtcagg ccggcgatga ccgcgtcggt caccacccgg tggctgcgcg
     3961 gatgggccag caccgggatg ccaccggcct cccggacgag gcgtaccgcg accgcggccg
     4021 gcgtgctgtg atggccgacg tagtagggcg agtcgtcgcg cagcaccgtg gcgaaggcct
     4081 ggtcgcggct ggcgaacacc cctgccgcca ctaaggcgtc ggcgatgtgt gggcgaccga
     4141 gcgtggtcgc gtcggtggtc tgcgcgcgca cggccgccat cgttaccggg aacccgtcgg
     4201 cggccagccg gcggaccatc tcctcggccc gcggcacccg gtcgtcgcgg gagagcgcca
     4261 tctcgcgggc cagcgcggga tggtcggggt cgaacaggta ggccagcagg tggaggctgt
     4321 cgggcccgtc ggcacaggtc agctcagcgc ccgggaccag gcatacgccc agccgggtcg
     4381 cggccgccgc tgcctcggcc caccccagca ccgtgtcgtg gtcggtgagg gcgaccgcgt
     4441 cgaggccggc cgcgacggcg gcggccatca gctcggcggg cgcctcggtg ccgtcggaga
     4501 ccgccgagtg ggtgtgcagg tcgatcctca cgccggcaac tctagggcga tcaccgacag
     4561 acccgccgtg acgatccaca gccgggttcg gtgccacagt gggaggcatg catgttccct
     4621 gcgtcggtgc cgtggtccac gacgacgacg gccggctgct ggtggtccag cgcggccacg
     4681 acccggcgat gggtcgctgg tcgatccccg gcgggcgggt cgaggccggc gagaccgacg
     4741 tacaggccgt ggcccgtgag gtgctggaag agactggcct gcacgtcacc gtcggggtcc
     4801 gcctcggcac ggtgcagctg cccggacctg acggggtggt ctatgacgtg cgcgactacg
     4861 cctgcgcgat cgcggtgccg ggcacgacgc cggtggccgg caatgacgcc gccgcggtgc
     4921 gctgggtcag ccgggtcgag ctcacgcacc ttgacctgac tgacggcctg gtcgacaccc
     4981 tcacccagtg gggcgccctc ccccactgac ccgcactttc ccccaccgac cccgcactcc
     5041 ccctgcactg atcatgcgca aatcatcggg cggagcgtcg cctcgcgcgc tcagtgcctc
     5101 agggctggct ggcgatctgg atgaggttgc cgcaggtgtc gtcgaagacc gcggtggtga
     5161 ccggacccat ctgcgtcggc tcctgggtga accggacgcc ctgcgccttg agccgctccg
     5221 cctcggccgg cacgtcggcg actgcgaacg acgtgaacgg gatgccgtcg gccgccaacg
     5281 cgtccttgaa cgggcggacc gccgggtggc tgtcgggctc gagcaccagc tcgacgccgt
     5341 ccgggtcctc cggcgagacc acggtgagcc agcgcgcgtc gcccagtggg atctcggtct
     5401 tcttcacgaa cccgagcacc tccgtgtaga agcgcaacgc cttgtcctgg tcgtcgacga
     5461 gcacgctcgc caggttgatc ctcatcgggg cttcctctcg gggtcgatgg gccagcggtc
     5521 ggtgatcgtg cgcagtggcg tcttgtcgag ccggtggaag gtgtagcggc cctcgcgatg
     5581 ggtggccacc aggccggcgt cctcgagcac cgcgatgtgc tgcgagatgg cttgacgcga
     5641 cgaggacagt ccgtgcttca tcgcgagtcg gccgcacagc tcgaacagtg tctggccgtc
     5701 gcgctcctgc aattcatcga gaatggcgcg tcgcgtcgag tcggccagtg ccttgaacac
     5761 gtcggccacg actcagacgt taggcaagtg gccgcttgcc tgtcaaggac tcggtacgac
     5821 gcgcgcgcac gatccatcat cgggctcgca caccacggtg accccgtctt tcagccccca
     5881 gaccgtgagg ctcaagacga ctcggtcgtc ggcgctccag cgcgcgcttg ccacagagac
     5941 gggcccgggc aacttcaaag ccacctgagc cccgctccag acatcgagca tggcgggggc
     6001 aatgccatac ctgccgtcga taaagccgcg ccgtccgtca tcggaaagtg caaccagccg
     6061 catgccgcac tcctccaacg cctggtgcgg cttgccgatc acctcccaca cctgcgagca
     6121 ccgggccccc ggcccctcga cgagaattcg gtgccccgat tccgccacgt cgagcacccg
     6181 cccggcggtg atgtcgacgg gcgctgcttc cggagcatcg gggtcccacc acgccacccg
     6241 gttggcccag ttgctcgcgt agacgtcgag gacgactcgc ccgtccagcc aaccggacac
     6301 ccgagtgccg ctgtcaagac ccgagacgcg actcagcatc cgccccgtgt cggcgtcgaa
     6361 gaccatcagc gtgatggtca gggacgggcc cgccttcgga tccggcacgg cggccgcgac
     6421 ggcaacccga tgccggccgc tgtccagtgc cgcgcctcgc ggcttgccag tcatgagcac
     6481 ctgcccggtc gcacgcaacc gagtcacggt gtgcgactcg tccgcatcgt tggacgtgtc
     6541 acccgggact atctccagca cgccccacgg atcgcctgtg aagctcaccg ggccctgtgc
     6601 ctcaggaaga ccctgaaacc aggcgagcac ctgtctatag gaggcgatag cgcgatgcga
     6661 gggaaccgct gaccggcctg tcacggatgg ctcgggcaac tgggtggcca tcgtcggacg
     6721 caccggcgcg tgggtgccgg aaacccatgc cggtacggcg accagggcga ccaccacaaa
     6781 gacggcggcc gctgccgcgc cgatcgctcg acgtcgccgc cgcgctcgcc cccgggccat
     6841 cactcggtcg gcaagcagcc cgacgtccat gcgctccatc acgcccgcct gctcctcgag
     6901 cgtggcgcgt agctgtgtct cgacgtccgc gttcatcgct caccacccga tggttcgacg
     6961 accggtgcgt cggcggcctg caaggcattg cgcagcgacg ccagcgcccg gtgcgcctgg
     7021 ctcttgacgg tgcctggggt gcagccgagc gtttgcgcga tctccgtttc ggacaggtcc
     7081 tcgtagtagc gcagcaccac caccgcccgc tgccgtggcg gcagcccgcg cagcagcgcc
     7141 cacatcgcga gtcgctccac ggcatccgcc gactcatcaa tgcccgccgc tgcggcggcc
     7201 aacccgtcgg gcacctcgcc gatcggtgtc tccgcgcgcc acttccgccg ccaccacgac
     7261 acgtgcgtgg tcaccatgac ccggcgcacg taggcgtcga tcgactcacg gtcgcgaatg
     7321 cgcccccagg ccagataggt cttggtcagc gccgtctgca gcaggtcttc ggccagctga
     7381 tggtcgccgg tcactaggta cgccgtacgg agcaggaggt gcgagcgagc cacgacgtac
     7441 gcgcgaaact ccgcatcgcg accaccgccc gcgccggatt cccccgcctc gcccaccatt
     7501 ccccctccgc gtcaccccct aggacgcgcc gacgccccga tcaggttgtc atgacggttt
     7561 gcgcatgatc agtgcaggag tgggcggcag gcggcgacgg tttgcgcatg atcagtgcag
     7621 agatgggcgg ccgggctgct cgccgccgcg cgcgtcgagg tagctctgct tgggcaccat
     7681 caccttgcgg cggaagatgc agaccagcgt gccgtcctgg ttgcagcc
//
LOCUS       DATIBB010000160         7702 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_888122, whole genome shotgun sequence.
ACCESSION   DATIBB010000160 DATIBB010000000
VERSION     DATIBB010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 7702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7702
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_888122"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..482)
                     /locus_tag="VLC50_06115"
     CDS             complement(<1..482)
                     /locus_tag="VLC50_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020574772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HSF27077.1"
                     /translation="MAELYYDEDADLSIIAKRRVAVLGFGSQGHAHALSLRDSGVDVR
                     VGLPEGSKSRAKAEAAGLRVVTPAQASAEADLIMVLAPDPVQRRLYAEDIEPNLSDGD
                     AIFFGHGFNIRFGYIKPPAGVDVCMVAPKGPGHLVRREFTEGRGVPDLVAVEQDATGN
                     A"
     gene            complement(546..1070)
                     /gene="ilvN"
                     /locus_tag="VLC50_06120"
     CDS             complement(546..1070)
                     /gene="ilvN"
                     /locus_tag="VLC50_06120"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006541007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HSF27078.1"
                     /translation="MSKHTLSVLVQNRPGVLARIAGLFSRRGFNIDSLAVGPTEHDDV
                     SRMTIVVNVDENPLEQVTKQLNKLVEVLKVVELETSSSVQRELLLIKVRADSDTRSHI
                     LETVELFRAKVVDVASDSLTIEATGNADKLDALLRMLDPYGIRELVQSGLVAVGRGPR
                     SITDRSLRTVERSA"
     gene            complement(1070..2824)
                     /locus_tag="VLC50_06125"
     CDS             complement(1070..2824)
                     /locus_tag="VLC50_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015796331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HSF27079.1"
                     /translation="MSDPSSEQVSGATSLIRSLEAVGVETVFGIPGGAILPAYDPLMD
                     SSRLRHILVRHEQGAGHAAQGYAQATGRVGVCMVTSGPGATNLVTPIADAMMDSVPIV
                     AITGQVPSGAIGTDAFQEADIRGITMPITKHNFLVTDVEDIPRTITEAFHIASTGRPG
                     PVLVDIAKDTLQASTHFRWPVEMDLPGYRPVTRPHAKQVREAARLIIEAKRPVLLVGG
                     GVIKADASAELRVLADLTGAPVVTTLMARGALPDSHRQNLGMPGMHGAVAAVTALQKA
                     DLIVSLGARFDDRVTGKLSTFAPHAAIVHADVDPAEISKNRYADVPIVGDAREVIADL
                     IVAVQAEHAAGHSADYGAWWAALDQWRTTYPLGYLEPPPGEVSPQRVIERIGQITGPD
                     TIYTAGVGQHQMWAAHYVRYEKPRTWLNSGGLGTMGYAIPAAMGAKVGMPDSTVWAID
                     GDGCFQMTNQELATCVVNDIPIKVAIINNGSLGMVRQWQTLFYNQRYSNTDLQSRRVP
                     DFVKLAEAYGCVGLRCDSPEQVDATIEKAMAIDDVPVVVDFVVHQDAMVWPMVPAGTS
                     NDEILAARGVRPDFGDDD"
     gene            complement(3123..4778)
                     /locus_tag="VLC50_06130"
     CDS             complement(3123..4778)
                     /locus_tag="VLC50_06130"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HSF27080.1"
                     /translation="MPSSPPEVVVIADLLGRLRFVSPALREVFGYDPASFDGQALLAA
                     VHPADLDDLRRALRRLLAERPTGPIRCRVRHADGVWRATEVSASTWRDTGAGPVVVLA
                     ARDVTDQLMLERVLAHRARHDALTELPNRRQVSEHLQSRLADPAEGDRLAVLVLGLDG
                     FATVNDTAGHLVGDALLVALARRLSSVVRAGELLARVDGDEFAVVLEDVHAEAAVLVA
                     TRLRAAVSRPVRLGERELSVRASVGVAFARPGTTPESLLRDADLALHRAKATGRDRVQ
                     VASPELHAELAGRVELESRLRAALARREFELHYQPIVDLLDGAMVGAEALLRWWRDPA
                     TLVPPDQFLHVAEASGLVAPLGWWAFDAAIAQLAAWRAEGLDVGMSVNLSAAQLGEPA
                     LAHTVLARLERAGVDPSALTLEVTESVLVEQLTAANRGLAALRAHGVTVALDDFGTGY
                     CGLGYLRELSVDVLKLDRSFVAGLGTDREHTVLVRAIVGLANDLGLLTVAEGVETVAQ
                     LRMLQELGAQLAQGFLFEAPQMPAHAAAHARVGFGAAVLSAAG"
     gene            complement(4799..6493)
                     /gene="ilvD"
                     /locus_tag="VLC50_06135"
     CDS             complement(4799..6493)
                     /gene="ilvD"
                     /locus_tag="VLC50_06135"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003934868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HSF27081.1"
                     /translation="MTEHDLKPRSRDVTDGLERAAARGMLRAVGMGDDDWEKPQIGVA
                     SSWNEITPCNLSLDRLAKAAKDGVHAAGGYPLEFGTISVSDGISMGHEGMHFSLVSRE
                     VIADSVETVMQAERLDGSVLLAGCDKSLPGMLMAAARLDLASVFLYAGSILPGRATLT
                     DGTEREVTIIDAFEAVGACARGLMSREDVDIIERAICPGEGACGGMYTANTMASAAEA
                     LGMALPGSAAPPATDRRRDQYARKSGEAVVELIRRGITARQILTKDAFENAIAVVMAL
                     GGSTNSVLHLLAIAREAEVDLTLDDFNRIGSKVPHLADVKPFGHYVMTDVDRIGGIPV
                     VMKALLDAGLLHGDALTVTGRTLAENLADIAPPDVDGKVVRALREPIHRTGGLTILRG
                     TLAPDGAVVKTAGFEADVFEGTARVFDGERAAMDALEDGTVGAGDVVVIRYEGPKGGP
                     GMREMLAITGAIKGAGLGKDVLLLTDGRFSGGTTGLCVGHVAPEAVDAGPIAFVRDGD
                     RIRLDVEAKTLDVLVDADELAARREGWEPLPPRYTRGVLAKYSRLVGSASIGAVCG"
     gene            complement(6527..6868)
                     /locus_tag="VLC50_06140"
     CDS             complement(6527..6868)
                     /locus_tag="VLC50_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005565526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like small modifier protein 1"
                     /protein_id="HSF27082.1"
                     /translation="MNAVGESISGDATGQVVVRLPGVLRELAEGQREVRVPVPRSARV
                     ADVLDRLVETRPVLARRLRDETGALRRHVNVYVDGEDVRRGAGLAEPVAPGAVIDVLP
                     SIAGGSSGETH"
     gene            complement(6865..>7702)
                     /locus_tag="VLC50_06145"
     CDS             complement(6865..>7702)
                     /locus_tag="VLC50_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018775351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HSF27083.1"
                     /translation="FPEDTETALARVWQLAPDSADRPGLVWAGVEPTSLWRSTDGGET
                     FELVRGLWDHPHRDQWTPGFGGAAVHTVLPDPADDARVLVAMSTGGVYVTADGGASWT
                     PSNDGISAYFLPDPNPEFGQCVHKVARDGAEPRRLYAQNHHGVYRSDDAGASWTSIAD
                     GLPSDFGFVMLASPRESGTAWVVPLVADGERVPPQGRLRVHRTRDAGDSWTELGDGLP
                     DAAWVSVLRDAACVDRADPTGVYLGTRDGTVYASADAGGHFVTVAEHLPDVLCVRAVV
                     PS"
BASE COUNT         1059 a         2758 c         2813 g         1072 t
ORIGIN      
        1 gcgttgccgg tggcgtcctg ctcgacggcg accaggtcgg gcacgccgcg accctcggtg
       61 aactcccggc gcaccaggtg gcccggcccc ttgggggcga ccatgcagac gtcgaccccg
      121 gccggcggct tgatgtagcc gaaccggatg ttgaagccgt gcccgaagaa gatggcgtca
      181 ccgtcggaca ggttgggctc gatgtcctcg gcgtagaggc gacgctgcac cgggtcgggc
      241 gccagcacca tgatcaggtc ggcctcggcg ctggcctgcg ccggcgtgac gacccgcagc
      301 ccggccgcct cggccttcgc ccggctcttc gagccctccg gcagaccgac gcgcacgtcg
      361 accccggagt cacgcagcga gagcgcatgg gcgtggccct ggctgccgaa tccgagtacg
      421 gcgacgcggc gcttggcgat gatcgagagg tcggcgtcct cgtcgtagta gagctcagcc
      481 acggtgcggg ttctccttca tgagtcgatc ggtgggtgcg agtggatggt gggtgaggcg
      541 agcgctcagg cgctgcgctc gacggtgcgc aacgaccggt cggtgatcga ccggggcccc
      601 cggccgacgg cgaccaggcc cgactgcacc agctcacgga tgccgtaggg gtcgagcatc
      661 cgcagcagcg cgtcgagctt gtcggcgttg ccggtcgcct cgatcgtcaa ggagtcgctc
      721 gcgacgtcga ccaccttggc ccggaacagc tcgaccgtct ccaggatgtg cgagcgggtg
      781 tcggagtcgg cgcgcacctt gatgagcagc agctcgcgct gcacggagct gctggtctcg
      841 agctcgacga ccttgagcac ctcgacgagc ttgttgagct gcttggtgac ctgctcgagc
      901 gggttctcgt cgacgttgac gacgatcgtc atccgggaga cgtcgtcatg ctcggtcggc
      961 ccgacggcca gcgagtcgat gttgaacccg cgccgggaga acaggccggc gatccgcgcc
     1021 agcacgccgg gccggttctg cacgagcacc gagagagtgt gcttgctcat cagtcatcgt
     1081 ccccgaagtc gggccgcacc ccacgcgcgg caaggatctc gtcgttgctg gtcccggccg
     1141 ggaccatcgg ccagaccatg gcgtcctggt ggacgacgaa gtcgacgacg accggcacgt
     1201 cgtcgatcgc catcgccttc tcgatcgtcg cgtcgacctg ctcgggcgag tcgcagcgca
     1261 ggcccacgca cccgtaagcc tcggccagct tgacgaagtc gggcacccgc cgcgactgca
     1321 ggtcggtgtt ggagtagcgc tggttgtaga acagcgtctg ccactggcgg accatgccga
     1381 ggctgccgtt gttgatgatg gccaccttga tcgggatgtc gttgacgacg caggtggcca
     1441 gctcctggtt ggtcatctgg aagcagccgt cgccgtcgat ggcccacacc gtggagtcgg
     1501 gcatgcccac cttggcgccc atcgccgcgg ggatcgcgta gcccatcgtg ccgagcccgc
     1561 cggagttgag ccaggtgcgc ggcttctcgt agcggacata gtgagcggcc cacatctggt
     1621 gctggccgac gccggcggtg tagatcgtgt ccggcccggt gatctgcccg atccgctcga
     1681 tgacccgctg cggcgagacc tctccgggcg gcggctcgag gtagcccagc gggtaggtgg
     1741 tgcgccactg gtcgagcgcg gcccaccagg ccccgtagtc ggcgctgtgc cccgcggcgt
     1801 gctcggcctg caccgcgacg atcagatcgg cgatcacctc gcgggcgtcg ccgacgatcg
     1861 gcacgtcggc gtagcggttc ttggagatct cggccgggtc cacgtcggcg tgcacgatcg
     1921 cggcgtgcgg ggcgaacgtc gacagcttgc cggtcacgcg gtcgtcgaac cgggcgccca
     1981 gcgagacgat caggtcggcc ttctgcaacg cggtgaccgc cgcgaccgcg ccgtgcatgc
     2041 cgggcatccc gaggttctgc cggtggctgt cgggcagcgc cccgcgggcc atcagcgtgg
     2101 tgaccaccgg cgcgccggtg agatcggcga gcacccgcag ctcggcggac gcgtcggcct
     2161 tgatcacccc gccgccgacc agcagcaccg gccgcttggc ctcgatgatc agccgggccg
     2221 cctcgcgcac ctgcttggca tgcgggcggg tgaccgggcg atagccgggc aggtccatct
     2281 cgaccggcca gcggaagtgg gtgctggcct gcagggtgtc cttggcgatg tcgaccagga
     2341 ccggcccggg ccgaccggtc gaggcgatgt ggaacgcctc ggtgatggtg cgcgggatgt
     2401 cctcgacgtc ggtcaccagg aagttgtgct tggtgatcgg catcgtgatg ccgcggatgt
     2461 cggcctcctg gaaggcgtcg gtgccgatgg ccccgctggg cacctgcccg gtgatcgcga
     2521 cgatcggcac cgagtccatc atggcgtcgg cgatcggggt gacgaggttg gtggccccgg
     2581 gcccgctggt caccatgcag accccgacgc ggccggtggc ctgcgcgtag ccctgcgcgg
     2641 cgtgcccggc accctgctcg tggcggacca ggatgtggcg cagccgcgac gagtccatca
     2701 gcgggtcgta cgccggcagg atcgcgccac cggggatccc gaacacggtc tcgacgccta
     2761 cggcctccag cgacctgatc aagctggtcg caccggacac ctgctcgctg ctcggatcac
     2821 tcatggtctg cgcccttcgc gttcggtctc gtcgtcggct cgtcgtcgta cgtcccggtc
     2881 atccgccgtg accgcggcag atgtcgtcac cgtggtcctg gcaacaaaaa acccctcggg
     2941 cccgaaggca tgtcgagggg tgcgcgctcg gcttgtcgtc agccagcgcg ccagggaagt
     3001 acgaggtcgg tgcgacgcat gacgaaaccg tgccgcaccg gaccgcgcga tgtcaaccgt
     3061 tcgagatcac cgtctcatca tccggaccgt tcgagggtgg ctgtggcact gcctgcgtga
     3121 ggtcagcccg ccgccgacag cacggccgct ccgaagccga cccgtgcgtg cgcggccgcg
     3181 tgggccggca tctgaggagc ctcgaagagg aagccctgag ccagctgggc gcccagctcc
     3241 tgcagcatgc gcagctgggc gaccgtctcg acgccctcgg ccacggtgag cagccccagg
     3301 tcgttggcca gcccgacgat ggcccgcacc agcacggtgt gctcgcggtc ggtgcccagc
     3361 ccggcgacga acgaccggtc gagcttgagc acgtcgaccg acagctcgcg caggtagccc
     3421 aggccgcagt agccggtgcc gaagtcgtcg agggccaccg tgaccccgtg cgcccgcagc
     3481 gcggccagcc cgcggttggc ggccgtgagc tgctcgacga gcacgctctc ggtgacctcc
     3541 agggtcagtg ccgacgggtc gacgcccgca cgctcgagcc gggccagcac cgtgtgcgcc
     3601 agcgccggct cgccgagctg ggccgccgac aggttgacgc tcatgccgac gtcgagcccc
     3661 tcggcccgcc aggccgccag ctgggcgatg gccgcgtcga aggcccacca cccgagcggg
     3721 gcgaccagcc cgctcgcctc ggcgacgtgc aggaactggt ccggcggcac cagggtggcc
     3781 gggtcgcgcc accagcgcag cagggcctcg gcacccacca tcgcgccgtc gagcaggtcg
     3841 acgatcggct ggtagtgcag ctcgaactcc cggcgggcca gggcggcgcg cagccggctc
     3901 tcgagctcga cccggccggc cagctcggcg tgcagctcgg gggaagccac ctgcacccgg
     3961 tcacgtccgg tggccttggc ccggtgcagc gccaggtcgg cgtcacgcag gagcgactcg
     4021 ggggtcgtgc cgggccgggc gaaggccacc ccgacgctgg cccgcacgct cagctcccgc
     4081 tcgccgaggc ggaccgggcg agagacggcg gcccgcagcc gggtggcgac caggactgcg
     4141 gcctcggcgt ggacgtcctc gagcacgacc gcgaactcgt cgccgtcgac ccgggcgagc
     4201 agctcgccgg cgcgcacgac cgagctcagc cggcgggcca gggcgaccag cagcgcgtcg
     4261 ccgacgaggt gcccggcggt gtcgttgacg gtggcgaacc cgtcgagccc gagcaccagc
     4321 acggcgaggc ggtcgccctc ggccgggtcg gcaagccgcg actgcaggtg ctcgctgacc
     4381 tgacgccggt tgggcagctc cgtcagcgcg tcatgacggg cccggtgggc cagcacccgc
     4441 tcgagcatga gctggtcggt gacgtcacgg gcggcgagca ccaccaccgg gccggctccc
     4501 gtgtcccgcc aggtgctcgc gctcacctcg gtggcgcgcc acacgccgtc ggcgtggcgg
     4561 acccggcacc ggatcgggcc ggtcggtcgc tcggcgagca gccggcgcag cgcccgccgt
     4621 aggtcgtcga ggtcggcggg atggacggcg gcgagcagag cctgcccgtc gaacgacgcg
     4681 gggtcatagc cgaagacctc tcgcagcgcc gggctgacga agcgcagccg gccgagcagg
     4741 tcagcgatga cgacgacctc gggcggggag ctgggcacgc gcgaggtcac aggcgaagtt
     4801 aaccgcagac tgccccgatc gaggcggaac cgacaagccg tgaatacttc gccagcacgc
     4861 cgcgggtgta gcgcgggggc agcggctccc agccctcccg gcgcgccgcc agctcgtcgg
     4921 cgtcgacgag cacgtcgagc gtcttggcct cgacgtcgag gcggatgcgg tcgccgtcgc
     4981 gcacgaacgc gatcggacct gcgtcgacgg cctcgggcgc gacgtggccg acgcacaggc
     5041 ccgtcgtacc gccggagaac cgcccgtcgg tgagcagcag gacgtccttg cccaggccgg
     5101 cacccttgat cgccccggtg atcgcgagca tctcgcgcat gcccggcccg cccttgggcc
     5161 cctcgtagcg gatcaccacg acgtcgccgg cgccgaccgt gccgtcctcg agcgcgtcca
     5221 tggccgcccg ctcgccgtcg aagacccggg cggtgccctc gaacacgtcg gcctcgaagc
     5281 cggcggtctt gaccaccgcg ccgtcggggg cgagcgtgcc gcgcaggatg gtcagcccac
     5341 cggtgcggtg gatcggctcg cgcagcgcgc gcaccacctt gccgtcgacg tcgggcggag
     5401 cgatgtcggc gaggttctca gccagggtgc gcccggtgac cgtcagcgcg tcgccgtgca
     5461 gcagcccggc gtcgagcagc gccttcatca ccaccgggat gccgccgatg cggtcgacgt
     5521 cggtcatcac gtagtgcccg aacggcttga cgtcggccag gtgcgggacc ttggacccga
     5581 tccggttgaa gtcgtcgagg gtgaggtcga cctcggcctc gcgggcgatc gcgagcaggt
     5641 gcagcaccga gttggtcgag ccgcccagcg ccatcacgac cgcgatcgcg ttctcgaacg
     5701 cgtccttggt caggatctgg cgggcggtga tgccgcggcg gatcagctcg acgaccgcct
     5761 cgccggactt gcgggcgtac tggtcacggc gccggtcggt ggccggcggg gcggcgctgc
     5821 cgggcagggc catgcccagc gcttcggccg cgctggccat cgtgttggcg gtgtacatgc
     5881 cgccgcaggc gccctcgccg gggcagatgg cccgctcgat gatgtcgacg tcctcgcggc
     5941 tcatcagccc gcgggcgcag gcgccgaccg cctcgaacgc gtcgatgatc gtgacctcgc
     6001 gctcggtgcc gtcggtgagg gtggcccggc cgggcaggat cgagccggcg tagaggaaca
     6061 cgctggccag gtcgaggcgg gccgcggcca tcagcatgcc gggcagcgac ttgtcgcagc
     6121 cggccagcag caccgaaccg tcgaggcgct cggcctgcat cacggtctcg accgagtcgg
     6181 cgatcacctc acgcgagacc agcgagaagt gcatgccctc gtggcccatc gagatgccgt
     6241 cggagaccga gatggtgccg aactcgagcg ggtagccgcc ggcggcgtgc acgccgtcct
     6301 tggcggcctt ggccagccgg tcgagcgaga ggttgcaggg ggtgatctcg ttccacgagc
     6361 tcgccacgcc gatctggggc ttctcccagt cgtcgtcgcc catgcctacg gcgcggagca
     6421 tgccgcgcgc ggcggcgcgc tcgaggccat cggtgacgtc acggctgcgg ggcttgagat
     6481 cgtgctcggt catggcgcga gtgtaggact gctgtccgac tggcgatcag tgcgtctcac
     6541 ctgacgaccc gccggcgatc gagggcagca cgtcgatgac cgcgcccggg gcgactggct
     6601 cggccagccc ggctccgcgc cgtacgtcct ctccgtcgac atagacgttg acgtgccggc
     6661 gcagcgcgcc ggtctcgtcg cgcagccgcc gggccagcac cggtctggtc tcgaccagcc
     6721 ggtcgagcac gtcggcgacc cgagccgacc gcggcaccgg cacccgcacc tcgcgctggc
     6781 cctcggccag ctcgcgcagg accccgggca gccgtacgac gacctgcccg gtcgcgtcgc
     6841 cactgatcga ttcgccgaca gcattcatga cggcacgacc gcccgcacgc acagcacgtc
     6901 gggcaggtgc tcagccaccg tgacgaagtg cccgcccgcg tcggcgctgg catagaccgt
     6961 gccgtcgcgt gtcccgaggt agacaccggt cgggtcggcg cggtccacgc aggccgcgtc
     7021 gcgcagcacc gagacccagg ccgcatcggg cagcccgtca ccgagctcgg tccagctgtc
     7081 ccccgcatcg cgggtgcggt gcacccgcag ccgcccctgc ggcgggaccc gttcgccgtc
     7141 ggccaccagc ggcaccaccc aggcggtgcc gctctcacgt ggggaggcca gcatcacgaa
     7201 cccgaagtcg ctgggcagcc cgtcggcgat cgaggtccag ctggccccgg cgtcgtcgct
     7261 gcgatagacc ccgtggtggt tctgcgcgta gagccggcgc ggctcggcgc cgtcgcgggc
     7321 caccttgtgc acgcactggc cgaactccgg gttggggtcg ggcaggaagt aggcggagat
     7381 cccgtcgttg ctgggggtcc acgacgcgcc gccgtcggcg gtgacgtaga ccccgccggt
     7441 gctcatcgcg accagcaccc gggcgtcgtc ggccgggtcg ggcagcacgg tgtgcaccgc
     7501 cgcaccgccg aagccgggcg tccactggtc gcggtgcggg tggtcccaca gcccgcggac
     7561 cagctcaaag gtttcgccgc cgtcggtgct gcgccacagc gaggtgggct ctacgccggc
     7621 ccacaccagg cccggccggt cagcgctgtc cggggccagc tgccacaccc gggccagcgc
     7681 ggtctcggtg tcctcgggga ac
//
LOCUS       DATIBB010000161         3867 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_362953, whole genome shotgun sequence.
ACCESSION   DATIBB010000161 DATIBB010000000
VERSION     DATIBB010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3867
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_362953"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..973)
                     /locus_tag="VLC50_06150"
     CDS             complement(<1..973)
                     /locus_tag="VLC50_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009740059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HSF27084.1"
                     /translation="MPEDPAASVTAERLRTQLLSGTPAAAAGDVVRRLLAVQAQDPRG
                     ARLAVRARSRGLHAADVDHAVSEERSLVVSWLNRGTLHLVAAEDFWWLRELTAPTMVS
                     TNARRLDQEGVPAADAERAVATIVGALERDGPLTRAQLREQVAPTGVRVAGQAMVHLL
                     LLATLRGAIVRGPIVGREQAFVLVRDWLGAPPKSLDTKRFAREAALAELAVRYLRGHA
                     PASERDLAKWAGLPVSAARAGLAAIAARLRHRPDGLLELRSQPVDGRGGRADLPGPRL
                     LGSYDPVLHGWVDRGPILGEHTSVVTSNGLFRPFALVSGRAVATWSWS"
     gene            999..1637
                     /locus_tag="VLC50_06155"
     CDS             999..1637
                     /locus_tag="VLC50_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013131694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03085 family metal-binding protein"
                     /protein_id="HSF27085.1"
                     /translation="MISFAADERARLCDQLLQGGPDAPTLCAGWTARDLAAHLVLRES
                     RPDASVGVAVRPLAGYTERVRLEIAARPWEQLVREVRGGPPLWSPMRIRAVGELVNAV
                     EFFVHHEDLRRAQPEWTPRPLEPSTQAMLWRTLQTGGGWLYRHGPVGVVLARPDGTRT
                     TARRARAGRSVTVTGEPAELVLHAFGRQAHALVELDGDPLDVAALAAARLGP"
     gene            complement(1638..1943)
                     /locus_tag="VLC50_06160"
     CDS             complement(1638..1943)
                     /locus_tag="VLC50_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012877890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF27086.1"
                     /translation="MSRSRVLLLGVLVLAVLVLASCAAGPNNVARVDAPEIAGFWQGL
                     WHGLILPITFIVSLFTDDVSIYDVHNDGNWYDFGFVLGLCILSSGSAGPRVAARRSR"
     gene            complement(2027..2800)
                     /gene="hisF"
                     /locus_tag="VLC50_06165"
     CDS             complement(2027..2800)
                     /gene="hisF"
                     /locus_tag="VLC50_06165"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017947481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HSF27087.1"
                     /translation="MSVAVRVIPCLDVDAGRVVKGVRFQELRDAGDPVEMARLYDGEG
                     ADELVFLDITASSSDRETTYDVVRRTAEEVFIPLTVGGGVRSVDDVDRLLRSGADKVG
                     VNTAAVARPELIAEIADRFGAQVLVLSVDARRARDGAATDSGFEVTTHGGRRGTGLDA
                     IAWAARAAELGAGEILLNSVDADGTRDGYDLQMLAAVRAAVAIPVIASGGAGALEHFA
                     PAVAAGADAVLAASVFHFGTLRIGQVKDTVAQAGFAVRR"
     gene            complement(2797..3189)
                     /locus_tag="VLC50_06170"
     CDS             complement(2797..3189)
                     /locus_tag="VLC50_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012853220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HSF27088.1"
                     /translation="MTRRVGSGTPWESIVGYSRAVAAGDHVWVSGCTSTVDGEVVHEG
                     DPHAQMRQALRNVEEALHALDAELADVVRTRIYVTDISRWEEVGRAHGEVFGAIAPAT
                     SMVQVAALIDPRMLVEVEALAFKRGSSW"
     gene            complement(3186..>3867)
                     /gene="priA"
                     /locus_tag="VLC50_06175"
     CDS             complement(3186..>3867)
                     /gene="priA"
                     /locus_tag="VLC50_06175"
                     /EC_number="5.3.1.16"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020552898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional
                     1-(5-phosphoribosyl)-5-((5-
                     phosphoribosylamino)methylideneamino)imidazole-4-
                     carboxamide isomerase/phosphoribosylanthranilate isomerase
                     PriA"
                     /protein_id="HSF27089.1"
                     /translation="QAVRLVQGEAGSATSYGDPLAAALAWQEAGAEWVHLVDLDAAFG
                     RGSNRELLADVVGRLDVAVELSGGIRDDASLEAALATGCARVNLGTAALEDPAWTAAA
                     IARHGDRVAVGLDVRGTTLAARGWTREGGDLWEVLERLGRDGCARFVVTDVTKDGTLR
                     GPNLDLLREVCARTDRPVVASGGVSSLADLQALRTLVPLGVEGAIVGKALYAGAFTLA
                     EALAEVSR"
BASE COUNT          536 a         1489 c         1393 g          449 t
ORIGIN      
        1 tcgaccacga ccaggtcgcc accgcccgcc cggagacgag cgcgaagggt cggaacaggc
       61 cgttgctggt gaccaccgag gtgtgctcgc cgaggatcgg cccgcggtcg acccaaccgt
      121 gcagcaccgg gtcgtaggag ccgagcagcc gcgggccggg caggtcggca cgcccgcctc
      181 ggccgtcgac gggctgtgac cgcagctcga gcagcccgtc ggggcgatgg cgcaaccgcg
      241 cggcgatcgc ggccaggccc gcccgggcag ccgagacagg caggccggcc cacttggcca
      301 ggtcccgctc gctggccggc gcatggccgc gcaggtagcg cacggccagc tcggccagtg
      361 ccgcctcgcg ggcgaaccgc ttggtgtcca gcgacttcgg cggcgcaccg agccagtcgc
      421 gcaccagcac gaacgcctgt tcccgcccga cgatcgggcc gcgcacgatg gctccgcgca
      481 gggtggccag caggagcagg tgcaccatcg cctggcccgc cacccgaacc ccggtcgggg
      541 cgacctgctc gcgcagctgt gcccgggtca gcggcccgtc gcgctcgagc gcccccacga
      601 tcgtggccac cgcccgctcg gcgtccgccg ccggcacgcc ctcctggtcg agccggcggg
      661 cattggtgct gaccatggtc ggcgccgtga gctcacgcag ccaccagaag tcctcggcgg
      721 cgaccaggtg caaggtgccg cggttgagcc acgacaccac cagcgatcgc tcctcgctca
      781 cggcgtggtc gacgtcggcg gcgtgcaggc cgcgggagcg ggcgcgcaca gccagccggg
      841 cgccgcgcgg gtcctgggcc tgcacggcga gcagccggcg tacgacgtca ccggccgcgg
      901 ctgccggcgt acccgagagc agctgggtgc gcaggcgctc ggccgtcacc gacgctgcgg
      961 ggtcttcagg catccgggtg cctgcctacg atcacgaggt gatctccttc gccgccgacg
     1021 aacgtgcacg cctgtgcgac cagctgctcc agggtggccc agacgcgccg accctatgcg
     1081 cgggttggac ggcgcgagac ctggccgcgc acctggtgct gcgcgagtcg cggcccgacg
     1141 cctcggtggg cgtggccgtg cgcccgctcg ccggctacac cgaacgcgtg cggctggaga
     1201 tcgccgcccg gccgtgggag cagctcgtgc gcgaggtgcg cggcggcccg ccgctgtggt
     1261 caccgatgcg gatccgcgcg gtgggcgagc tggtcaacgc cgtggagttc ttcgtacacc
     1321 acgaggacct gcgccgagcc cagcccgagt ggacaccccg tccgctcgag ccttcgaccc
     1381 aggcgatgct gtggcggacc ctgcagacgg gaggcgggtg gctctaccgg cacggcccgg
     1441 tcggggtcgt tctggcgcgg cctgacggga cacgaacgac ggcacgccgc gcgcgggccg
     1501 gccgctcggt caccgtcacg ggcgagccgg ccgagcttgt cctgcacgcg ttcggccgac
     1561 aggctcacgc actggtcgag ctcgacggtg acccgctcga cgttgctgcg ctcgctgcgg
     1621 cccgcctcgg tccgtgatca gcgtgagcgg cgggcggcga cacgcggccc ggcggagccc
     1681 gaggacaaga tgcaaaggcc cagcacgaag ccgaagtcgt accagttccc gtcgttgtgc
     1741 acgtcgtaga tgctcacgtc gtcggtgaac agcgacacga tgaacgtgat cggcaggatc
     1801 agcccgtgcc agagaccctg ccagaacccg gcgatctccg gggcgtcgac ccgggccacg
     1861 ttgttgggcc cagccgcgca gctggccagc accagcaccg cgagcacgag gactcccagc
     1921 agcagcacgc gcgaacggct catggcccac ccccgcggcc aaaccaaccg gagcgtgggc
     1981 gcggtcacaa cccgtcgaag gtcccgacgg ggtcagcgga gcggcgtcaa cggcgtacgg
     2041 cgaagccggc ctgcgcgacc gtgtccttga cctgcccgat gcgcagcgtg ccgaagtgga
     2101 acacgctggc cgcgagcacc gcgtcggccc ccgcggcgac ggccggcgcg aagtgctcga
     2161 gcgccccggc cccgccggag gcgatcacgg gaatggcgac cgcggcgcgg accgccgcca
     2221 gcatctgcag gtcgtagccg tcacgggtgc catcggcgtc gaccgagttg agcaggatct
     2281 cgcccgcgcc cagctcggcg gcccgagcgg cccaggcgat cgcgtcgagc ccggtgccgc
     2341 gccgcccgcc gtgcgtggtc acctcgaacc cggagtcggt ggcggcgccg tcgcgcgccc
     2401 ggcgggcgtc caccgacagc acgagcacct gcgcgccgaa ccggtcggcg atctcggcaa
     2461 tcagctcggg acgggccacc gccgcggtgt tgaccccgac cttgtcggcg cccgagcgca
     2521 gcagccggtc gacgtcgtcg accgagcgca ctcccccgcc cacggtcagc gggatgaaca
     2581 cctcctcggc ggtgcgccgg accacgtcgt aggtcgtctc ccggtcgctg ctcgaggcgg
     2641 tgatgtcgag gaacaccagc tcgtcggcgc cctcgccgtc gtagagccgg gccatctcga
     2701 ccgggtcgcc ggcgtcacgc agctcctgga aacgcacccc cttgaccacc cggccggcgt
     2761 cgacgtcgag gcacgggatg acgcgtacgg cgacgctcac cagctcgacc cccgcttgaa
     2821 agcgagcgcc tcgacctcga ccagcatccg cgggtcgatg agcgcggcca cctgcaccat
     2881 cgacgtggcc ggggcgatcg cgccgaacac ctcgccgtgg gcccggccga cctcctccca
     2941 gcgggagatg tcggtgacgt agatccgggt gcgtacgacg tcggccagct cggcgtcgag
     3001 cgcgtgcaag gcctcctcga cgttgcgcag cgcctggcgc atctgggcgt gcgggtcacc
     3061 ctcgtggacc acctcgccgt cgaccgtgga ggtgcagccg gagacccaca cgtggtcgcc
     3121 ggcggccacg gcccggctgt agccgacgat cgactcccac ggcgtgcccg acccgacccg
     3181 ccgcgtcacc tcgacacctc ggcgagcgcc tcggcaagcg tgaacgcgcc ggcatagagc
     3241 gccttgccca cgatggcccc ctccacccct agtggcacga gcgtgcgcaa cgcctgcagg
     3301 tcggccagcg aggacacccc gccgctggcc accaccggcc ggtcggtgcg cgcgcacacc
     3361 tcgcgcagca ggtcgaggtt gggcccgcgc agcgtgccgt ccttggtcac gtcggtgacg
     3421 acgaaccggg cgcagccgtc ccggccgagc cgctcgagca cctcccacag gtcgccgccc
     3481 tcgcgggtcc agccgcgggc ggcgagggtg gtgccgcgca cgtcgagccc gaccgccacc
     3541 cggtcgccgt ggcgggcgat cgccgcggcg gtccaggccg ggtcctccag cgccgcggtg
     3601 cccaggttga cccgagcaca cccggtggcc agcgccgcct ccaacgaggc gtcgtcgcgg
     3661 atccccccgg agagctcgac cgcgacgtcg aggcggccca ccacgtcggc gagcagctcg
     3721 cggttggacc cgcggccgaa cgcggcgtcg aggtcgacca ggtggaccca ttcggcgccg
     3781 gcctcctgcc aggccagcgc ggcggccagc gggtcgccgt acgacgtcgc actgccagcc
     3841 tcgccctgca ccaggcgcac cgcctgg
//
LOCUS       DATIBB010000162         3520 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_889063, whole genome shotgun sequence.
ACCESSION   DATIBB010000162 DATIBB010000000
VERSION     DATIBB010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3520
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_889063"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(277..3249)
                     /locus_tag="VLC50_06180"
     CDS             complement(277..3249)
                     /locus_tag="VLC50_06180"
                     /inference="COORDINATES: protein motif:HMM:NF024867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HSF27090.1"
                     /translation="MRLHRLAVTAFGPFAGTETVDFDALTEAGLFLLHGSTGAGKTSV
                     LDAVCFALYGVVPGARQQGGHLRSDHAAPDVRPEVVLDVSVAGRRVEIIRSPAWERPK
                     RRGSGTTTEPARTLVRERRDGEWIDLTSRNDEAGQLVQSLTGMNASQFTQVVMLPQGR
                     FAEFLRADAETRRLILEQLFVTHRFSDVETWLADQRRVLDRAVTAADQAIGELLARVA
                     EAADAAPPDGEIAPATWLDTLCTEAAALVTQETQAADWARAELRAAEQALRDAERVAG
                     LQARHAEAMTSMTVLEAAEPEQRQRRAELTAARRAAVIAGHLHAVHRCTDRLANATSA
                     AEAAVAAVVSLMPDIEPEPMPLRLAAKGLREEAGRLGELARDAEELARVRAELAQTQA
                     GQAAETAELDVAQAFLSGADTRRVALARELVEMREAAAGMESAELSASAARRQLTAVR
                     QLAKLRPRQSAAADGVRDAIDIAQAARERWLALRAARLDGMAAELAEGLVDGHPCPVC
                     GGFEHPQPATSTAEAVRDVDEAQAREEFETADRGRVAAQDTLAAVTAEVVAASARAVG
                     DTEDALPDETELLTKIDALEATLEQARAALARVPDLERAIDDLDAEVERTRSRHQELL
                     TAAATRLERISALEQRIQEIDAMLIAAVGPGVDPLARAAEVTASADTAHAAAEALLRR
                     AEAEADLETATGALAVAAAEASFADVEGAVSALREEDAVATLEQRCRDHDDARARVSA
                     TLEDAEVAAAAQLPAADLTPLRERQDVLASLHEAAVGRLDAATRRQSALDRLSVELTA
                     ASAESAPLRERARLVGELSRLAEGTGGGNTLRMRLSSYVLAARLEAVAEAASARLLDM
                     SGGRYTLVHSAEAGGRGRAGLGLRIVDAWTGADRHPSTLSGGESFFASLALALGLADV
                     VAQEAGGARIETLFVDEGFGSLDDETLDEVMDTLDALRDGGRAVGLVSHVGDLRQRVT
                     AQLHVIKGRAGSHLRQSS"
     gene            complement(3249..>3520)
                     /locus_tag="VLC50_06185"
     CDS             complement(3249..>3520)
                     /locus_tag="VLC50_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003805868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D C-terminal
                     domain-containing protein"
                     /protein_id="HSF27091.1"
                     /translation="QVTLTDPVRPREPMERLRVRFPHTLVLGFDPEGGAADDVRSYTE
                     RLRGLDDLDVCCSFVEHVRHSPATPDEVDVLREAFEALRVEQASA"
BASE COUNT          450 a         1319 c         1293 g          458 t
ORIGIN      
        1 cagcccgtcc agcccggcgg ccagccccgg gccggggtag accccggcca ccgccgacgc
       61 gacctgcgcg tcgccggggc tgccccacgg ctcaaacggc tcgaacacgt gtacgacact
      121 agccggaccc accgacagac aaggccacac cgacaagccg caccgacacg ccgcaccgac
      181 acgccgcacc cagcagccgc accagccggc ccgatcaggc gggggaggga ggtgatgtgc
      241 gagctcgaag ggagggcgcg cgcgagcgtc ggctggctag ctcgactggc gcaggtggga
      301 gccggcgcgg cccttgatca cgtgtagctg tgcggtcacg cgttggcgga ggtcacccac
      361 gtggctgacg agcccgaccg cccggccacc gtcgcgcagc gcatcgaggg tgtccatgac
      421 ctcgtcgagg gtctcgtcgt cgagcgagcc gaacccctcg tcaacgaaga gcgtctcgat
      481 ccgggcgccg cccgcctctt gcgcgacgac gtcagccagg cccagcgcga gcgccagcga
      541 ggcgaagaag ctctcgcccc ccgacagggt cgacgggtgc cgatcggcgc cggtccacgc
      601 gtcgacgatg cgcagaccga ggcccgcccg tccccgcccg ccggcctcgg ccgagtgcac
      661 caacgtgtag cgccccccgg acatgtcgag cagccgggcg ctggcggcct cggcgaccgc
      721 ctcgagacgc gcggcaagga cgtaggacga cagcctcatc cgcagcgtgt tgccgccgcc
      781 ggtgccctcg gcaagacgcg acagctcacc gacgagccgg gctcgctcgc gcagcggcgc
      841 cgactcggcg ctggccgcgg tcagctcgac cgagagtcgg tccagggcgc tctggcgccg
      901 ggtcgccgcg tccaaccgcc ccacagccgc ctcgtgcagg ctcgcgagga cgtcctgccg
      961 ctcgcgcagg ggggtcaggt cggcagcggg cagctgggcg gcggcggcca cctctgcgtc
     1021 ctcgagcgtc gcgctgaccc gggcgcgggc gtcgtcgtgg tcccgacagc gctgctcgag
     1081 ggtggccaca gcgtcttcct cacgaagggc gctgactgcc ccctccacgt cggcgaagct
     1141 ggcctctgcg gctgcgacgg ccaacgcccc ggtcgcggtt tcgaggtcgg cctcggcctc
     1201 ggcccgacgt agcaacgcct cggccgcagc atgcgccgtg tcggcgctcg cggtgacctc
     1261 ggcggcacga gccagcgggt cgacgcccgg cccgactgcg gcgatgagca tcgcgtcgat
     1321 ctcctggatc cgttgctcga gcgcggagat gcgttcgagc cgggtcgcgg cggcggtgag
     1381 cagctcttgg tggcgggagc gcgtgcgctc gacctcggcg tcgaggtcgt cgatcgcacg
     1441 ctcgaggtcg ggcacgcgtg ccagggcggc gcgagcctgc tccagtgtgg cctcgagcgc
     1501 gtcgatcttt gtgagcagct cggtctcatc gggtagggcg tcttcggtgt cgccaacggc
     1561 gcgggctgac gcggcgacca cctctgcggt caccgccgcg agcgtgtcct gtgccgcgac
     1621 ccgtccgcgg tcggcagtct cgaactcctc ccgcgcctgt gcctcgtcga cgtcgcgcac
     1681 cgcctcggcc gtgctggtcg cgggctgcgg gtgctcgaac ccgccgcaga cggggcacgg
     1741 gtggccgtcg acgaggccct cggcgagctc ggccgccatg ccgtcgagcc gagccgctcg
     1801 cagcgccagc cagcgctcac gggccgcctg ggcgatgtcg atggcgtcgc gaacaccgtc
     1861 ggccgcagcg gattggcgtg gtcgcagctt cgccagctgg cgcaccgccg tcagctgccg
     1921 gcgcgccgcg ctggcactca gctcggccga ctccattccg gctgcggcct cacgcatctc
     1981 gaccagctca cgcgccagcg ccacccggcg cgtgtcggct ccggagagga aggcctgcgc
     2041 cacgtcgagc tctgcagtct cagcggcctg cccggcctgc gtctgcgcca gctctgcccg
     2101 cacccgggcc agctcctcgg cgtcgcgggc cagctcgccg agccggcccg cctcttcacg
     2161 caaccctttg gcggccagac gcagcggcat cggttcgggc tcgatgtcgg gcatcagcga
     2221 gacgaccgcg gcgacggccg cctcggccgc tgatgtggcg ttggcgagcc ggtcggtgca
     2281 gcgatgcacc gcgtggagat gccctgcgat gacggcggcg cgtcgcgcag cggtcagttc
     2341 ggcgcggcgt tgccgctgct cgggctcggc ggcctcgagc acggtcatag acgtcatcgc
     2401 ctcggcgtgg cgagcctgca gcccggccac tcgctcggcg tctcgcagcg cctgctcggc
     2461 cgcacgcagc tcggcgcggg cccagtcggc cgcctgggtc tcctgggtga ccagggcggc
     2521 ggcctccgtg cagagggtgt cgagccaggt agccggtgcg atctcaccgt cgggtggtgc
     2581 ggcatcggcc gcctcggcca cccgggccag cagctcgccg atggcctggt cggctgcggt
     2641 caccgcccgg tcgaggaccc ggcgctggtc ggcgagccac gtctcgacgt cggagaagcg
     2701 gtgagtgacg aacagctgct cgaggatgag ccggcgggtc tcggcgtcgg cgcgcaggaa
     2761 ctcggcgaac cggccctgcg gcagcatgac cacctgggtg aactgcgagg cgttcatgcc
     2821 ggtcagcgac tgcaccagct ggccggcctc gtcgttgcgg gaggtgaggt cgatccactc
     2881 accgtcgcgg cgctcgcgca ccagcgtgcg cgccggctcg gtggtggtgc cgctgccacg
     2941 acgcttgggc cgctcccacg ccggcgagcg gatgatctcg acccgtcggc cggccaccga
     3001 cacgtcgagc accacctcgg ggcgcacgtc gggcgcggcg tggtcgctgc gcaggtgccc
     3061 gccctgctga cgggcgccgg gcaccacgcc gtagagcgcg aagcagaccg cgtcgagcac
     3121 gctggtcttg ccggccccgg tcgacccgtg cagcaggaac agcccggcct cggtcagcgc
     3181 gtcgaagtcg acggtctccg tgccggcgaa cgggccgaac gcggtgacgg caagccggtg
     3241 cagccgcatc aggcgctcgc ctgctcgacc cgcagcgcct cgaacgcctc gcggagcacg
     3301 tcgacctcgt cgggcgtggc gggggagtgg cgcacatgct cgacgaacga gcagcacacg
     3361 tcgaggtcgt cgagcccgcg cagccgctcg gtgtaggacc gcacgtcgtc ggccgcgcca
     3421 ccctccgggt cgaacccgag caccagggtg tgcgggaatc ggactcgcag tcgctccatc
     3481 ggctcgcgcg ggcgcaccgg gtcggtcagc gtgacctgca
//
LOCUS       DATIBB010000163         6901 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_627237, whole genome shotgun sequence.
ACCESSION   DATIBB010000163 DATIBB010000000
VERSION     DATIBB010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 6901)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6901)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6901
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_627237"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            152..1348
                     /gene="dusB"
                     /locus_tag="VLC50_06190"
     CDS             152..1348
                     /gene="dusB"
                     /locus_tag="VLC50_06190"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018556670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HSF27092.1"
                     /translation="MTTSADVEADATDVALRPLPLGRHQVWPPVVLAPMAGITNSPFR
                     LLCREQAEAAAASAGTGAGLYVSEMITSRALVERDAETMRMIRFAPAESPRSIQLYGV
                     DPAVIGKAVAMVVDEDLADHVDLNFGCPVPKVTRQGGGSALPWRRRLFREIVRAAVAS
                     AGGLVPVTVKMRKGIDDEHLTYLDAGRIAEQEGVAWVALHARTAAQHYGGTADWEAIA
                     RLKEHVTSIPVLGNGDVWEGADALSMLERTGADGVVVGRGCLGRPWLFADLAAAFAGT
                     PRTVLPSLREVMATMRRHAELLCEHFGPDKGCREIRKHIAWYTKGFAVGSTTRRALAM
                     VSSLAELDALIATLDLDQPFPREVLGLPRGRTTGSRHVVLPEGWLDSPDALDVPEGAE
                     LDISGG"
     gene            1442..4177
                     /gene="ppdK"
                     /locus_tag="VLC50_06195"
     CDS             1442..4177
                     /gene="ppdK"
                     /locus_tag="VLC50_06195"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015033490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HSF27093.1"
                     /translation="MCVVCREQAVPKFVYDFSEGNKDLKDLLGGKGANLAEMTNLGLP
                     VPPGFTITTEACKVYLATGEEPADLRDEVSTHLTALEQTMGKQLGQPGDPLLVSVRSG
                     AKFSMPGMMDTVLNIGLTDESVAGLAKQAGDERFAWDAYRRLLQMFGKTVLGVDGDRF
                     EHALEAAKSARGTHNDLDLDAADLRRLVEEFKAIIAADSGREFPQDPREQMDLAVRAV
                     FESWNSERAILYRRQERIPADLGTAVNVVAMVFGNLGMDSGTGVAFTRDPATGHPGIY
                     GDYLQNAQGEDVVAGIRNTMALADLEAIDKTSYDELLAIMATLENHYRDLCDIEFTIE
                     RGKLWMLQTRVGKRTAAAAFAIATQLVDQGLIDIDEALTRVTGAQLAQLMFPRFNGGA
                     GRVRLTKAVAASPGAAVGQAVFDSERAAALAATGVPVILVRRETNPDDLKGMIAAQGV
                     LTSRGGKTSHAAVVARGMGKTCVCGADALEVDTKAARFTTSDGTVVSEGDVISIDGTS
                     GEVFLGEVPVAPSPVVRYFEGELDPAAPDADELVVAVHRIMSHADATRRLGVRANADT
                     PEDAARARRFGAGGIGLDRTEHMFLGERRQHVEDLILATTEAERRAALDALLPLQRDD
                     FRGIFAAMDGLPVTVRLIDPPLHEFLPDLTELSVEVALAAERGQGDTDEARRRAKLLE
                     AVRRLHEQNPMLGLRGVRLGLVIPGLFAMQVRAIAEAAASLVQEGKDPRPEIMIPLVG
                     AIQELEAAKDEALAVLAEVRAATGVDLTPPLGTMIELPRAALTAGQIAEAADFFSFGT
                     NDLTQTTWGFSRDDVEGAFFSRYLELGVFGVSPFESIDPDGVGRLVRIAAEEGRAANP
                     NLHLGICGEHGGDPDSVHFFHKAGLDYVSCSPFRVPVARLEAGRAAVSTAGSDSR"
     gene            4287..5543
                     /locus_tag="VLC50_06200"
     CDS             4287..5543
                     /locus_tag="VLC50_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007497159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA];
                     GO_process: GO:0006203 - dGTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HSF27094.1"
                     /translation="MADTSSVSGCALRYDDAARARWASEPGKRPGRTPFERDRARVLH
                     SAALRRLASKTLVVLAGEHDFLRTRLTHTLEVAQIGRELAKALGADPDLVDAACLAHD
                     LGHPPFGHNGEAALAGLADGCGGFEGNAQTLRVLTRLEAKTFDASGRSVGLNLTRAAL
                     DAATKYPWTRAHRADKFGVYDDDLEVFRWARDGAPEGRRCLEAQVMDWADDVAYSVHD
                     VEDAVHAGRLRFEALHDPDERAALLALTATRFPEARTDELGAALDRLLALPVWPTSYD
                     GSLPALAALKDLTSQLIGRFAVAAEEATRASYGDRPLTRYDADLVVPRPVRLECEVLK
                     AVADRYVMQPRMAHGAYDDERRVVVELVQALLERAPHALEPWLRPAWAAAADDAARRR
                     VVIDQVASYTDTSAIAAHRRLVRGIG"
     gene            5602..6825
                     /locus_tag="VLC50_06205"
     CDS             5602..6825
                     /locus_tag="VLC50_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012888630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HSF27095.1"
                     /translation="MSVEQTFVVVGASLAGTKAVEGMREAGFDGRIVLVGKEKDLPYD
                     RPPLSKGLLKGETPDDEVFLHEPGWYGEARVELRLGTPVDAIDRAAHEVELAGGERLH
                     YDKLLLATGSMPRKLPLPGADLAGVHYLRRLGDARAIRDAVTRGGRVVIVGAGWIGLE
                     VAAGARHHGASVTVVEPAPTPFYGVLGERLGTVFADLHRRNGVDLRTGTGVEAIEGTD
                     TVTGVRLTGGEVVPADAVIIGVGARPNTRLAEAAGLAVDGGVLVDEWLRTSDPDIYAA
                     GDIASVAHALIGERVRVEHWANANDQGLAAGRSMAGAGAPWDVLPFFYTDQYDLGMEY
                     HGWVGAGGSDDLVIRGDVDALDFDAFWLRENQVLAGMHVNRWDDSDPIKELARTRAKV
                     DRDRLADPAVPLSEL"
BASE COUNT          961 a         2506 c         2499 g          935 t
ORIGIN      
        1 cgtggaagat tgcagcgcca gagcctaggt aatttgactc gatcttcgcg atctgacgag
       61 gtggggcgca cgtaggcgtc gacgtacaac gattgcttga tcgcgccgcc gagtggaacg
      121 ggcccggcgc gtgggttgcg acaatggcgc ggtgaccact tctgccgacg tcgaagctga
      181 cgccaccgac gtcgccctac gcccgctgcc gttgggccgc caccaggtct ggccgccggt
      241 ggtgctggcg ccgatggccg gcatcaccaa cagcccgttc cggctgctct gccgcgagca
      301 ggccgaggcc gcagccgcct cggcgggcac cggggccggg ctctatgtct ccgagatgat
      361 cacttcgcgg gcgctggtcg agcgcgacgc cgagaccatg cggatgatcc ggttcgcgcc
      421 ggcggagtca ccgcgcagca tccagctcta tggcgtcgac ccggcggtga tcggcaaggc
      481 cgtcgcgatg gtggtcgacg aggacctggc cgaccacgtg gacctcaact tcggctgccc
      541 ggtgcccaag gtgacccgcc agggtggcgg cagcgcgctg ccgtggcggc ggcggctgtt
      601 ccgcgagatc gtgcgcgccg cggtcgcctc ggccggcggc ttggtgccgg tgacggtcaa
      661 gatgcgcaag ggcatcgacg acgagcacct gacctatctc gacgccgggc ggatcgccga
      721 gcaggagggc gtcgcctggg tggcgctgca cgcgcgtacg gcggctcagc actacggcgg
      781 caccgccgac tgggaggcca tcgcccggct caaggagcac gtgacgtcca tcccagtctt
      841 gggcaacggc gacgtctggg agggtgccga tgcgctgtcc atgctcgagc gcaccggcgc
      901 cgacggtgtt gtcgtgggcc ggggctgcct cggccggccc tggctgttcg ccgacctcgc
      961 ggccgcgttc gccggcacgc cgcgcacggt gctgccgagc ctgcgcgagg tcatggcgac
     1021 gatgcgtcgg cacgccgagc tgctgtgcga gcacttcggt cccgacaagg gttgccggga
     1081 gatccgcaag cacatcgcct ggtacacgaa gggatttgcc gtcggctcga ccactcgccg
     1141 cgcactggcg atggtctcga gcctggccga gctcgacgcc ctgatcgcca cgctcgacct
     1201 cgaccagccc ttcccgcgcg aggtgctcgg actgcctcgc gggcgcacca ccgggtcacg
     1261 gcacgtcgta ctgcccgagg ggtggctcga ctcgccggac gccctcgacg taccggaggg
     1321 tgccgagctc gacatcagcg gcggctgacc gcccggatcc cgggcgcggt ccggtgcgcc
     1381 agcaccgtca tcgtgatcat tggcgcctcg gtagagtcgc ggcaccgggg tccggaaagc
     1441 gatgtgcgtc gtgtgtaggg agcaggccgt gccgaagttc gtctacgact tctcagaggg
     1501 aaacaaggac ctcaaagact tgctgggggg caagggggcc aaccttgccg agatgacgaa
     1561 cctcgggctc ccggtgccgc ccggattcac catcacgacc gaggcctgca aggtctatct
     1621 ggccaccggc gaggagccgg ccgacctgcg cgacgaggtc tcgacccacc tgacggccct
     1681 cgagcagacg atgggcaagc agctcggtca gcccggggac ccgctgctgg tgtcggtgcg
     1741 atccggtgcc aagttctcca tgcccggcat gatggacacc gtcctcaaca tcgggctgac
     1801 cgacgagtcg gtggccgggc tggccaagca ggccggcgac gagcggttcg cctgggacgc
     1861 ctatcggcgg ctgctgcaga tgttcggcaa gaccgtgctg ggcgtcgacg gcgaccggtt
     1921 cgagcacgcc ctcgaggcgg ccaagtcggc ccggggcacc cacaacgacc tcgacctcga
     1981 cgccgccgac ctgcgccggc tcgtcgagga gttcaaggcg atcatcgcgg ccgactccgg
     2041 tcgggagttc ccgcaggacc cgcgcgagca gatggacctc gccgtacgcg ccgtgttcga
     2101 gtcgtggaac tccgagcggg cgatcctcta ccgccgccag gagcgcatcc cggccgacct
     2161 gggcaccgcg gtcaacgtcg tggccatggt gttcggcaac ctcggcatgg actccggcac
     2221 cggcgtcgcc ttcacccgcg acccggccac cggccacccc ggcatctacg gcgactacct
     2281 gcagaacgcg cagggcgagg acgtggtcgc tggcatccgc aacacgatgg cgctggccga
     2341 tctcgaggcg atcgacaaga cgtcgtacga cgagctgctc gcgatcatgg cgacgctcga
     2401 gaaccactac cgcgacctgt gcgacatcga gttcacgatc gagcgcggca agctgtggat
     2461 gctgcagacc cgcgtgggca agcgcaccgc agcagcggcc ttcgcgatcg ccacccagct
     2521 ggtcgaccag gggctcatcg acatcgacga ggcgctgacc cgggtcaccg gagcccagct
     2581 ggcccagctg atgttccccc gcttcaacgg cggggccggt cgggtgcggc tgaccaaggc
     2641 ggtcgccgcc tcgccgggcg ccgcggtcgg gcaggcggtc ttcgactccg agcgggcggc
     2701 cgcactcgcc gcgaccgggg tgccggtgat cctcgtgcgt cgcgagacca accccgacga
     2761 cctcaagggc atgatcgccg cgcagggcgt gctgaccagc cggggcggca agaccagcca
     2821 cgccgcggtg gtggcccgcg ggatgggcaa gacctgcgtc tgcggcgccg atgcgctcga
     2881 ggtggacacc aaggcggccc ggttcaccac ctccgacggg acggtcgtct ccgagggcga
     2941 tgtcatctcg atcgacggca ccagcggcga ggtgttcctc ggcgaggtcc cggtcgcgcc
     3001 gtctccggtg gtgcgctact tcgagggcga gctcgacccg gcggcccccg acgccgacga
     3061 gctggtcgtc gcggtgcacc ggatcatgtc ccacgccgac gcgacccgcc ggctcggcgt
     3121 gcgcgccaat gccgacaccc ccgaggacgc ggcccgcgcc cgccgcttcg gtgccggcgg
     3181 catcggcctg gaccgcaccg agcacatgtt cctcggcgag cgccgccagc acgtcgagga
     3241 cctcatcctc gccaccaccg aggccgagcg ccgcgccgcg ctcgacgccc tgctgccgct
     3301 ccagcgcgac gacttccgcg gcatcttcgc cgcgatggac gggctcccgg tcacggtgcg
     3361 gctcatcgac ccgccgctgc acgagttcct gcccgacctg accgagctgt cggtcgaggt
     3421 ggcgctggcc gccgagcggg gccagggcga caccgacgag gcccgccggc gggccaagct
     3481 gctcgaggcg gtgcgccggc tgcacgagca gaacccgatg ctcggcctgc gcggggtgcg
     3541 gctcggtctg gtcatcccgg gcctgttcgc catgcaggtg cgcgcgatcg ccgaggccgc
     3601 agccagcctg gtccaggagg gcaaggaccc ccgccccgag atcatgatcc cgctcgtcgg
     3661 ggcgatccag gagctggagg ccgccaagga cgaggcgttg gccgtgctgg ccgaggtgcg
     3721 cgctgccacc ggggtcgacc tgacgccgcc gctgggcacc atgatcgagc tgccgcgggc
     3781 cgcgctgacc gcgggccaga tcgccgaggc cgccgacttc ttctcgttcg gcaccaacga
     3841 cctgacccag accacctggg gcttctcgcg cgacgacgtc gagggcgcgt tcttctcccg
     3901 ctacctcgag ctgggcgtct tcggcgtctc gccgttcgag tcgatcgacc ccgacggggt
     3961 gggccggctg gtgcggatcg ccgctgagga gggccgcgcc gccaacccca acctgcacct
     4021 gggcatctgc ggtgagcacg gcggcgaccc cgactcggtc cacttcttcc acaaggcggg
     4081 gctcgattac gtctcctgct cgccgttccg ggtgccggtc gccaggctcg aggccggccg
     4141 ggccgcggtg agcaccgccg gcagcgacag ccgctgaccc ctcgtcggtg agcatgcaat
     4201 gtgtggacgt cgtacacgga ttgcgtgatc accgaggacg ggcgcagacg ccgaagaccg
     4261 gtgcggatcg ctaggctcgc gccgtcatgg ccgacacgtc gagcgtctcc gggtgcgccc
     4321 tgcgctacga cgacgcggcg cgcgcccggt gggccagcga gcccggcaag cgtcccggcc
     4381 gcaccccgtt cgagcgtgac cgggcccggg tcctgcacag cgcggcgctg cgtcggctgg
     4441 ccagcaagac cctggtggtg ctcgccggcg agcacgactt cctgcgcacc cggctgaccc
     4501 acacgctcga ggtcgcccag atcggccgtg agctggccaa ggcgctgggg gccgaccccg
     4561 acctggtcga cgccgcctgc ctggcccacg acctgggcca cccgccgttc gggcacaacg
     4621 gcgaggcggc cctggccggg ctcgccgacg gctgtggcgg gttcgagggc aacgcgcaga
     4681 cgctgcgggt gctgacccgg ctggaggcca agacgttcga cgcgtcgggg cgcagcgtgg
     4741 ggctcaacct gacccgggcg gcgctcgacg ccgccaccaa gtacccgtgg acccgcgccc
     4801 accgggccga caagttcggg gtctacgacg acgacctcga ggtgttccgc tgggcccgcg
     4861 acggtgcgcc cgagggccgg cggtgcctgg aggcccaggt gatggactgg gccgatgacg
     4921 tggcctactc ggtgcacgac gtcgaggacg ccgtgcacgc cggacggctg cggttcgagg
     4981 cgctgcacga ccccgacgag cgggccgcgc tgctggcgct gaccgccacc cgcttcccgg
     5041 aggcccgcac cgacgagctc ggcgccgcgc tcgaccggct gctcgcgctg ccggtctggc
     5101 ccacgtcgta cgacggatca ttgcccgccc tggccgcgct caaggacctc acgagccagc
     5161 tcatcggccg gttcgccgtc gccgccgagg aggccacccg ggcgtcgtac ggcgaccgcc
     5221 cgctcacccg ctacgacgcc gatctcgtcg tgccgcgccc ggtccggctg gagtgcgagg
     5281 tgctcaaggc ggtcgccgac cgctacgtca tgcagccgcg gatggcccac ggcgcctatg
     5341 acgacgagcg ccgggtcgtg gtcgagctcg tccaggccct gctcgagcgg gccccacacg
     5401 cccttgagcc gtggctgcgt ccggcctggg cggccgcggc cgacgacgcg gcgcgccggc
     5461 gcgtggtgat cgaccaggta gcgtcctaca ccgacacctc agcgatcgcg gcgcaccgtc
     5521 ggctcgtccg cggaatcggc tgaaccaccc gagggttctt gccgtgtcat gcacaacagg
     5581 gacagatgag aggcaggcag gatgagcgtg gagcagacct tcgtggtggt gggggcgagc
     5641 ctggccggta cgaaggcggt cgagggcatg cgcgaggccg ggttcgacgg caggatcgtg
     5701 ctggtcggca aggagaagga cctgccctat gaccggcccc cgctgtcgaa gggtctgctc
     5761 aagggcgaga cgcccgacga cgaggtcttc ctccacgagc cgggctggta cggcgaggcg
     5821 cgggtcgagc tgcgactggg cacccccgtc gacgccatcg accgggccgc ccacgaggtg
     5881 gagctggcgg gcggcgagcg gctgcactac gacaagctgc tgctggccac cggatcgatg
     5941 ccccgcaagc tgccgctgcc cggtgccgac ctggccggcg tgcactacct gcgtcggctc
     6001 ggcgacgcgc gggcgatccg tgacgcggtc acccgcggcg gccgggtcgt catcgtcggg
     6061 gccggctgga tcggcctgga ggtcgcggcg ggcgcgcgtc accacggcgc gtcggtgacc
     6121 gtcgtcgagc cggcacccac cccgttctac ggcgtgctcg gcgagcggct cgggacggtg
     6181 ttcgccgacc tgcaccggcg caacggcgtc gacctgcgca ccggcacggg ggtcgaggcc
     6241 atcgagggca ccgacaccgt gaccggcgtg cggctgaccg gcggcgaggt cgtgccggcc
     6301 gacgccgtca tcatcggcgt gggagcccga cccaacaccc ggctcgccga ggcggccggg
     6361 ctcgccgtcg acggcggcgt gctcgtcgac gagtggctgc gcacctctga ccccgacatc
     6421 tacgccgccg gcgacatcgc ctcggtcgcg cacgcgctga tcggcgagcg ggtccgggtc
     6481 gagcactggg ccaacgccaa cgaccagggt ctcgcggccg gccggtcgat ggccggtgcc
     6541 ggcgcgccgt gggacgtgct gccgttcttc tacaccgacc agtacgacct cggcatggag
     6601 tatcacggct gggtcggcgc cggcgggtcc gacgacctcg tgatccgcgg cgacgtcgac
     6661 gccctggact tcgacgcgtt ctggctgcgc gagaaccagg tgctcgccgg catgcacgtc
     6721 aaccgctggg acgactccga cccgatcaag gagctggccc ggacccgggc caaggtcgac
     6781 cgcgaccggc tggccgaccc cgccgtacct ctctccgagc tctgagcgcc gccccggact
     6841 cacggcgtca ccgccactcc ggtcgtggtc atgcacgcgg gcgcgccccc ccgccccgcg
     6901 g
//
LOCUS       DATIBB010000164         3754 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_231656, whole genome shotgun sequence.
ACCESSION   DATIBB010000164 DATIBB010000000
VERSION     DATIBB010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3754)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3754)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3754
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_231656"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1253)
                     /locus_tag="VLC50_06210"
     CDS             complement(<1..1253)
                     /locus_tag="VLC50_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012924356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HSF27096.1"
                     /translation="MQPTVIVLSLAVTLVAVALFARAAARIVAVVRLGKPDPTRFGDA
                     GQRWTTMLRETLGHTRMLQWTKIGIAHWFVFVGFGALFFTLVTAYGQLFSPSFAIPLL
                     GHWVVYEWATEIIGWATLLGILALIGVRLANRPGHPTRRGRSSRFEGSTMWQGYYVEW
                     TIVAIAVCVLSLRGLEHALAGESGWSAHFPLTSWLGSAMSGLSTSATEDAIVAVAGIK
                     IAVSMAWFIVLAANLTMGVAWHRFLAFPNIWFKRHADGRTSLGALQPIVVDGKPVDFE
                     NIEELDENAALGVGKIEDFTWKGLLDFSTCTECGRCQDQCPAWNTQKPLSPKLVILGL
                     RDHAYARAPYLLAAQAAGANAAAGSGDGPGATMVDFGPDVPNETGPLIGTPEEGGVID
                     PDALWSCVSCGACVQQCPVDIEHVD"
     gene            complement(1352..2887)
                     /locus_tag="VLC50_06215"
     CDS             complement(1352..2887)
                     /locus_tag="VLC50_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014177131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid ammonia-lyase"
                     /protein_id="HSF27097.1"
                     /translation="MCSDLVINGAPLAPADLAAVARGQRSVRADDAVPARMTAAREAV
                     LTVAAAQEVYGRTTGVGANLAVHVESSTDDPLAHDRRLLASHATTGGPAYPDEVARAA
                     MVVRANQLARGMSGASPAGYLGLVAAVRAGAVPLVHRWGGVGTGDLGALAELARALVG
                     DVPWRSAGSPAVGPVVLAPGDVLALLSSNAVTIAQAALAAADLAELLAATEAVAALTL
                     VASAGNLEAFDPAVHAARPHPGAVRAAASVSALLAGARITPRRVQDRFGLRAFPAVHG
                     AALDALTGLEAVLAVETAAGAENPLVVADPAPRVLHHGSWHEAPLALALDHLRLAVVG
                     TATLATRRLSLLLDSAEHGLLDRGEGGGGEGEGEGLAFLAAEPDGNGAMGLDYVAAAA
                     LAHLRALAGPVSLGSAVLSLGAEDHASFAPEAARLTAESVDPLRTVLAAELVATVRAL
                     RLRRLDPAELGPAPVAGALRTSYDVLPHLAADRPLTEDVTLATSLLPGLAAVVQTLGV
                     TDE"
     gene            2937..>3754
                     /locus_tag="VLC50_06220"
     CDS             2937..>3754
                     /locus_tag="VLC50_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018157828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HSF27098.1"
                     /translation="MIEFEAVTKRYPDGTVAVNALSMTAPTGAITVLVGPSGCGKTTS
                     LRMVNRMIEPTGGRILVDGQDTAAADPAVLRRGMGYVIQHAGLFPHRTVLDNVATVPL
                     LLGEDRRTARARAHEVLERVGLDAALAQRYPAQLSGGQQQRVGVARALAADPPIMLMD
                     EPFSAVDPVVREALQDEFLRLQAELGKTIIFVTHDIDEAIKLGDQVAVLRQGGVLAQR
                     AAPAELLAHPYDDFVAGFVGRDRGYRALSFVAAGRPRLHPEPTVPVGASPAQARR"
BASE COUNT          520 a         1425 c         1370 g          439 t
ORIGIN      
        1 tggtcgacgt gctcgatgtc gaccgggcac tgctgcacgc aggcgccgca gctcacgcat
       61 gaccacagcg cgtcggggtc gatgaccccg ccctcctccg gcgtgccgat cagcgggccg
      121 gtctcattcg gtacgtcggg cccgaagtcg accatcgtcg ccccggggcc atcgcccgag
      181 ccggccgccg cgttcgcgcc cgccgcctgc gcggcgagca ggtagggggc tcgggcgtag
      241 gcgtggtcgc gcagcccgag gatgaccagc ttgggcgaga gcggcttctg ggtgttccag
      301 gccgggcact ggtcctggca gcggccgcac tcggtgcagg tggagaagtc gagcaggccc
      361 ttccaggtga agtcctcgat cttgccgacg cccagcgcgg cgttctcgtc gagctcctcg
      421 atgttctcga agtcgaccgg cttgccgtcg accacgatcg gctgcagcgc gcccagcgac
      481 gtccgcccgt cggcgtgacg cttgaaccag atgttgggga aggccaggaa ccggtgccag
      541 gccacgccca tggtcaggtt ggccgccagc acgatgaacc aggccatcga caccgcgatc
      601 ttgatgccgg ccaccgcgac gatcgcgtcc tcggtcgccg acgtggacag ccccgacatc
      661 gccgagccga gccaggacgt cagcgggaag tgcgcgctcc agcccgactc gccggccagc
      721 gcgtgctcga gcccgcgcag cgacagcaca cagacggcga tcgcgacgat cgtccactcg
      781 acgtagtagc cctgccacat cgtcgaaccc tcgaaccggc tcgaccggcc gcgacgggtc
      841 gggtgcccgg gccggttggc cagccgtacg ccgatcagcg cgaggatgcc gagcagcgtg
      901 gcccagccga tgatctcggt ggcccactcg tagaccaccc agtgccccag cagcgggatc
      961 gcgaacgacg gcgagaacag ctgcccatag gcggtgacca gggtgaagaa cagcgcgccg
     1021 aagccgacga acacgaacca gtgcgcgatg ccgatcttgg tccactgcag catccgggtg
     1081 tgcccgagcg tctcgcgcag catcgtggtc caccgctgcc cggcgtcgcc gaaccgcgtc
     1141 gggtcgggtt tgcccagccg caccaccgcg acgatccggg ccgccgcgcg ggcgaacagg
     1201 gccactgcga cgagcgtgac ggccaacgag aggacgatca cggtgggctg catgcggtcg
     1261 tgctccttgc gatcggaaac cgcaccgggg tgcgggtcgt gcgcgcttgc aggggcgaaa
     1321 agccgaggtc gagcctatgg cgcgctcgag gttactcgtc ggtaacaccc agcgtttgga
     1381 ccacggcggc gagcccgggc agcagggacg tggcgagcgt cacgtcctcg gtcagcggcc
     1441 ggtcggcggc gaggtggggc aggacgtcgt acgacgtccg cagcgcgccc gccaccggcg
     1501 ccgggccgag ctcggccggg tcgagccggc gcagccgcag cgcccggacc gtggcgacca
     1561 gctcggcggc cagcacggtg cgcagcgggt ccaccgactc ggcggtcagc cgggcggcct
     1621 ctggcgcgaa cgacgcgtgg tcctcggccc cgagcgagag caccgccgag ccgagcgaga
     1681 ccgggccggc cagcgcccgc aggtgggcca gcgccgcggc cgcgacgtag tccagcccca
     1741 tcgccccgtt gccgtccggc tcggcggcca ggaacgccaa cccctcgccc tccccctcgc
     1801 cgcccccacc ctcgccgcga tcaagcaatc cgtgctcagc cgagtccagc agcaggctca
     1861 gccgccgggt ggccagggtc gcggtgccga ccaccgccag ccgcaggtgg tcgagcgcca
     1921 gggcgagcgg cgcctcgtgc caggagccgt ggtgcagcac ccgcggcgcg gggtcggcga
     1981 cgaccagcgg gttctcggcg ccggccgcgg tctccaccgc gagcaccgcc tcgaggccgg
     2041 tcaacgcgtc gagcgccgcg ccgtgcaccg ccgggaacgc ccgcagcccg aaccggtcct
     2101 gcacccgccg cggcgtgatc ctcgccccgg ccagcagggc cgacaccgac gccgccgccc
     2161 gcaccgcgcc cgggtgcggc cgggcggcgt gcaccgccgg gtcgaacgcc tcgagattgc
     2221 cggcgctcgc gaccagggtc agcgcggcca ccgcctcggt ggcggccagc agctcggcca
     2281 ggtcggccgc ggccaacgcg gcctgagcga tcgtgaccgc gttgctcgac agcagggcca
     2341 gcacgtcgcc gggcgccagc accaccggcc caaccgcggg cgagccggcc gaccgccacg
     2401 gcacgtcgcc gaccagcgcc cgcgccagct cggccagcgc gcccaggtcg ccggtgccga
     2461 cgccgcccca ccggtgcacc agcggcaccg cgccggcgcg caccgcggcg accagcccga
     2521 ggtagccggc cggcgaggcc ccggacatcc cgcgggccag ctggttggcc cgcaccacca
     2581 tcgccgcccg cgccacctcg tcggggtagg ccggcccgcc ggtcgtggcg tgcgaggcca
     2641 gcagccggcg gtcatgggcc agcgggtcgt cggtggagct ttcgacgtgt acggcgaggt
     2701 tggcgccgac ccccgtcgta cggccataga cctcctgcgc cgctgcgacc gtcagcaccg
     2761 cctcgcgcgc ggccgtcatc cgggccggga ccgcgtcgtc ggcccgcacc gaccgctgtc
     2821 cgcgggccac cgcggctagg tcggccgggg ccagcggggc accgttgata acaaggtcgc
     2881 tgcacatgtg gccagtctgc tgcgcggatg tcggtgccgt gggagaggct ggacgggtga
     2941 tcgagttcga agcggtgacc aagcgctacc ccgacggcac cgtcgcggtc aacgcattga
     3001 gcatgaccgc gccgaccggg gcgatcaccg tgctggtcgg cccgtccggg tgcggcaaga
     3061 ccacgtcgct gcgcatggtc aaccgcatga ttgagccaac tggcgggcgg atcctcgtcg
     3121 acggacaaga caccgcggcc gccgacccgg cggtgctgcg ccgcggcatg ggctacgtca
     3181 tccagcacgc cgggctgttc ccgcaccgca ccgtgctcga caacgtggcc accgtgccgc
     3241 tgctgctcgg cgaggaccgg cgcaccgcgc gcgcccgggc ccacgaggtg ctcgagcggg
     3301 tcgggctcga cgccgcgctc gcgcagcgct acccggccca gctctccggc gggcagcagc
     3361 agcgggtcgg ggtggcccgg gcgctggccg ccgacccgcc gatcatgctg atggacgagc
     3421 cgttcagcgc ggtcgacccg gtggtgcgcg aggcgctgca ggacgagttc ctgcggctgc
     3481 aggccgagct gggcaagacc atcatctttg tcacccacga catcgatgag gcgatcaagc
     3541 tcggcgacca ggtcgctgtg ctgcgccagg gcggcgtgct ggcccagcgg gccgcgccgg
     3601 ccgagctgct ggcgcacccg tacgacgact tcgtggccgg cttcgtcggc cgcgaccggg
     3661 gctatcgggc gctctcgttc gtcgccgccg gccggccgcg gctgcacccg gagccgaccg
     3721 tgccggtggg cgcctcgccg gcgcaggccc gccg
//
LOCUS       DATIBB010000165        25641 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_1153491, whole genome shotgun sequence.
ACCESSION   DATIBB010000165 DATIBB010000000
VERSION     DATIBB010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 25641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25641
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1153491"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(300..1577)
                     /gene="tyrS"
                     /locus_tag="VLC50_06225"
     CDS             complement(300..1577)
                     /gene="tyrS"
                     /locus_tag="VLC50_06225"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016638143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HSF27099.1"
                     /translation="MTPANELLDDLQWRGLIVQSTDLGALREALDQGMLTYYCGFDPT
                     APSLHIGNLVQILTLRRLQLAGHRPLALVGGATGLIGDPGGRSTERTLNPRELVATWV
                     GRLRAQIQPFLTFEGPNAAVMVNNLDWIGQLSAIDLLRDIGKHFRVNKMITKDAVSAR
                     LNSEAGISYTEFSYQVLQGMDFLELYRRHGCLLQTGGSDQWGNLTAGLDLIHRVEGVS
                     AHALATPLVTKADGTKFGKTASGSVWLDPDMTSPYAFYQFWLNTDDRDVANYLKVFSF
                     RPREEIEALAAAVESRPAAREAQRALAAEITALVHGEKAMAAVVEASQALFGRGELAS
                     LDERTLADSLAELPRVSVSAPVPAYVDLLASSGLVSSKSAARRTVAEGGAYLNNRKVD
                     DGDYVPTAADLLAGRWMVLRRGRRALAAVELTS"
     gene            complement(1616..2272)
                     /locus_tag="VLC50_06230"
     CDS             complement(1616..2272)
                     /locus_tag="VLC50_06230"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018541195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HSF27100.1"
                     /translation="MLARAFFDRPVLEVAPDLLGAVITSHTQAGMVSVRLTEVEAYDG
                     SGHDPGSHAFRGRTKRNAVMFGPPGHCYVYFSYGMHWCVNLVCREEGWASAVLLRGGE
                     VVVGEELARARRPAARVARDLARGPARLASALGLDGTWNGLDVVTPGSPLLIRRGQVP
                     TSGVCQGPRVGVGGDGAERPWRFWLEAEPTVSPYRAHAPRHRRPAPGTVAGPDRGRAG
                     "
     gene            complement(2341..3807)
                     /gene="argH"
                     /locus_tag="VLC50_06235"
     CDS             complement(2341..3807)
                     /gene="argH"
                     /locus_tag="VLC50_06235"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013132269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HSF27101.1"
                     /translation="MADHTNHTRDGALWGGRFSGGPAGALAALSKSTHFDWRLAPYDL
                     LASRSHARVLHRAGLLTDDELDRMLEALGDLAAAVRTGTFVATEADEDVHSALERGLI
                     ERLGALGGKLRAGRSRNDQIATDLRLYLREAVRDVVGALAGLETALITQAATHRNAPA
                     PGMTHLQHAQPILFAHQLLAHVQAFKRDAERLADWDKRAAVCPLGAGALAGSSLAVDP
                     VTVAAELGFDRPADNSIDAVSDRDFAAEFLFAAALLGVHLSRLGEELCLWTTTEFGWV
                     ELDDAWATGSSIMPQKKNPDVAELARGKSGRLVGHLTGFLTTLKGLPLAYDRDLQEDK
                     EPVFDAVEQLLLVLPAMAGTIATMTVRTDRLAECAPQGFTLATDVAEWLVRQGVVFRD
                     AHEIAGALVRRCVARGVELWELADAELADVDARLTPAVREILTVRGALQARATHGGTS
                     PDRVAEQLAALTQAVADHAAWAEVRPVPAPAAQRGGVA"
     gene            complement(3807..5237)
                     /gene="argG"
                     /locus_tag="VLC50_06240"
     CDS             complement(3807..5237)
                     /gene="argG"
                     /locus_tag="VLC50_06240"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HSF27102.1"
                     /translation="MSKVLTSLPVGERVGIAFSGGLDTSVAVAWMRENGAVPCTYTAD
                     IGQYDEADVDGVPARALQYGAELARVVDCRAPLVEEGLAALACGAFHIRSGGRAYFNT
                     TPLGRAVTGTMLVRAMRADGVDIWGDGSTFKGNDIERFYRYGLLANPSLRIYKPWLDK
                     AFVSQLGGRAEMSRWLVEHDLPYRDSAEKAYSTDANIWGATHEAKTLESLDVSLETVD
                     PIMGVRFWDPAVVIEPEDVTVRFEHGRPVAINGTTYDDPVALVHEANAIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLWAVYERLLNAIHNEDTVASYHTQGRRLGRLLYEG
                     RWLDPQSLMLRESIQRWIASVVTGEVTLRLRRGEDYTVLRTSGPGFSYHPHKLSMERT
                     DNAAFGPTDRIGQLTMRNLDIADSRALLEAYAAQPLDQGQLLVEHGTLFGALPAGGAD
                     RISDNPTVAGDEESALDNAAMETGTD"
     gene            complement(5234..5812)
                     /locus_tag="VLC50_06245"
     CDS             complement(5234..5812)
                     /locus_tag="VLC50_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018540197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HSF27103.1"
                     /translation="MRPTSPRVPATPAARRQRIADVLARHEVRSQGELARLLAEDGIE
                     VTQATLSRDLDELGAVKVRAGAGPLVYAVPGEGGDRTPRPGGQAGDDGRLARLCGELL
                     VSAQASANLVVLRTPPGAAQFLASAIDRADLEAVLGTIAGDDTVLLICRGPDGAALAT
                     ELLALAEGGDAAPDESAPQPQIHSHTTRRNTP"
     gene            complement(5839..6768)
                     /gene="argF"
                     /locus_tag="VLC50_06250"
     CDS             complement(5839..6768)
                     /gene="argF"
                     /locus_tag="VLC50_06250"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014741211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HSF27104.1"
                     /translation="MSAPRHFLRDDDLTPAEQAQVLDLADAMKADRFGFRPLEGPRTV
                     AVLFDKHSTRTRVSFSVGIAELGGYPLVIDAQSSQLGRGEPIEDTTRVLDRQAVAIVW
                     RTFGQERIEAMAAVSRVPVVNALTDQFHPCQILADLQTVREHKGRLAGLTLTYLGDGA
                     NNMAHSYLLGGALAGMHVRVGSPEGFVPDSAVLARAQKIAAETGGSAAYVLDPHAAVE
                     GADVLATDTWVSMGQEGDGTDRAAPFLPYALDEAALDRAAPDAIVLHCLPAYRGKEIS
                     ASVIDGPASVVWDEAENRLHAQKALLAWLLERS"
     gene            complement(6765..8027)
                     /locus_tag="VLC50_06255"
     CDS             complement(6765..8027)
                     /locus_tag="VLC50_06255"
                     /EC_number="2.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012923000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine transaminase"
                     /protein_id="HSF27105.1"
                     /translation="MTAIDPLTELTAGGGNDEWTKRYGDALMGTFGTPKRVLVRGEGA
                     WVWDADGRRYLDLLGGIAVNALGHAHPLVVAAVTSQLATLGHVSNYFATRPQVALAER
                     LLELLDARGDGRVFFTNSGTEANEAAFKLTRRTGRTRLVAAEHAFHGRSMGALALTWK
                     PAYREPFAPLPGEVTFVPYGDTAALAAAVDGDTAAVFLEPIQGEAGVVVPPDGYLAAA
                     REITASQGALLVLDEVQTGIGRTGEWFAHTRHGVRPDVITLAKGLGGGLPIGATVALG
                     PAASLLGPGQHGTTFGGNPVAAAAALAVLQVIERDGLLAHVREVGQRLRERIAALGHP
                     LVDGVRGQGLLLAVTLTAPVAARVAAEAMAAGYVVNDVTPQALRLAPPLVLTAEQVDA
                     FVADLPAILDAAAPAGRAGSAGTQGDVS"
     gene            complement(8024..8920)
                     /gene="argB"
                     /locus_tag="VLC50_06260"
     CDS             complement(8024..8920)
                     /gene="argB"
                     /locus_tag="VLC50_06260"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013132274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HSF27106.1"
                     /translation="MSTRHAAAREKAATLVEALPWLQRFHGKVAVIKYGGHAMADEEL
                     RRSFAEDVMFLWYVGLRPVIVHGGGPQITAHLDRLGIDTEFRGGLRVTTPEAMDVVRM
                     VLVGQVQRNVVGLVNEHGPFAVGVSGEDARLFTAERRGALVDGELVDVGLVGDVVHVE
                     PGVVQGLVADGRIPVVSSIARGEDGQVYNVNADTAAAALAVALGAEKLVVLTDVEGLY
                     SSWPGDGAGGEPAELISSLHAGELEDMLPTLSSGMVPKMEACLRAVRGGVPKAHVLDG
                     RVPHALLLEVFTDSGVGTEVVA"
     gene            complement(8917..10068)
                     /gene="argJ"
                     /locus_tag="VLC50_06265"
     CDS             complement(8917..10068)
                     /gene="argJ"
                     /locus_tag="VLC50_06265"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019325166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HSF27107.1"
                     /translation="MSVTLPQGFRATGVAAGLKHSGDPDVALVVNDGPRHDAAGVFTS
                     NRVKAAPVMWSRQVVSDGRVDAVVLNSGGANACTGAAGFQDTHRTAERAAELLGLSAG
                     DLAVCSTGLIGERLPMDALLAGVERAVGELSDDGGEAAARAIMTTDTVPKQASYAGPG
                     FTVAGMAKGAGMLAPALATMLVVLTTDAVAEPADLDAALREGTRHSFDRVDADGCMST
                     NDTVLLLASGASGVSPSRAELTEAVEAVCLDLAHQLVADAEGASKEITVEVVRAADEA
                     DALEVGRAVARSALFKCAVHGEDPNWGRVLSALGTTKARFEPEHVAVAINGVWVCRDG
                     GVGADRSLVDMSARAVTVTIDLGAGDTTASIWTTDLTADYVHENSAYSS"
     gene            complement(10065..11096)
                     /gene="argC"
                     /locus_tag="VLC50_06270"
     CDS             complement(10065..11096)
                     /gene="argC"
                     /locus_tag="VLC50_06270"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011292480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HSF27108.1"
                     /translation="MGMRAAVAGASGYAGGELLRLLVAHPELEVGALTAGANAGARLG
                     TLHHHLPTLADRTLEPTTPEVLAGHDVVFLALPHGASAAVAAQLPEDVVVIDCGADFR
                     LADPAAWRQFYGTEHAGTWPYGLPELPGARARLAGARRIAVPGCYPTSVTLALAPAFT
                     EHLAEPDDVVVVAASGTSGAGRSLKPHLLGSEVMGAMSPYAVGGTHRHTPEIEQNLSG
                     CAGAPVTVSFTPTLAPMPRGILATCTARLAAGVDPAAVRVAYERAYADEPFVHLLPEG
                     QWPTTAGTLGSNTVHVQVTVDARVGRLIAVAAIDNLTKGTAGAAVQCANLALGLPETL
                     GLPVTGVAP"
     gene            11263..12174
                     /locus_tag="VLC50_06275"
     CDS             11263..12174
                     /locus_tag="VLC50_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004018707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HSF27109.1"
                     /translation="MITVEGLSKSYGGYLAVDDVTFACQPGAVTGFLGPNGAGKTTTM
                     RIIAGLTPPSRGDARIGGVHYRDIPNPGTQVGILLDASAQHAGRTGREVLTLGAETMG
                     LPRARVDEMLELVSLTPKEAGRRVRNYSLGMRQRLGIAHALLGDPKVLVLDEPANGLD
                     PAGIRWMRGLLRDYADRGGTVLLSSHLLHEVEAVADELLVIGKGRIVARGSKAELLAG
                     AGTLVRALDNTLLADALARAGLEASPGADGCLRTPATPEQVGRAAAAAGVVLVELRTA
                     DAGLEDMFLELTADTQRDELTPTGASA"
     gene            12171..12971
                     /locus_tag="VLC50_06280"
     CDS             12171..12971
                     /locus_tag="VLC50_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012834473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSF27110.1"
                     /translation="MSTTATSTSTAAPAIDVRQPSVPMLRLVKVELRKLVDTRAGFWL
                     AGSIVIISALITAGMLIWAEPQHLYFGDLFGVMNIPTGVLLPVLAILLVTSEWGQRTG
                     LVTFTLEPRRSRVVVAKLATSLVAAAGAVVVALAFGAVGAVLAGVLRDGAGGWEITGA
                     GLGNSILLQLLALLEGFGFAMLIMNSAAAIVLFFALPTAWMIIASIVPWLHEHVQPWA
                     DLATAQTPFQGPDLASASEWAHLGVASVIWIALPLALGIWRLLRAEVK"
     gene            complement(13124..15649)
                     /gene="pheT"
                     /locus_tag="VLC50_06285"
     CDS             complement(13124..15649)
                     /gene="pheT"
                     /locus_tag="VLC50_06285"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014175137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HSF27111.1"
                     /translation="MRVALSWLRDYVNLPATADGREVAERLLRAGLEVETVERVGDVS
                     GPLVVGRVLDFVEEPQKNGKTIRWCQVRVRPEGTGTEVEPEVRGIVCGAHNFAVGDHV
                     VVALPGTVLPGPFPITARKTYGHLSDGMIASVRELGIGEDHSGILVLDPAAAVGADAA
                     ELLGLGDEVLDIAVTPDRGYCLSVRGVAREAATAFDVPWRDPADLATDGANVSTGGYP
                     VAVDDPTGCDAFVAVRVSGFDPAAPSPDWLARRVRAGGMRSISLAVDVTNYVMLELGQ
                     PLHAYDAARLTGAIVVRRAREGERLRTLDDVDRALDPTDLLITDGSGPIGLAGVMGGQ
                     ATEIGERTSEIVIEAAHFDPVSIARTARRHRLPSEASRRFERGVDPAVALAAALRAAR
                     LLVEHGGGVLAPDVTLVQTPREPVVIELDITHPTRLVGRPYTDDEVVGALEAVGAVVE
                     RDGQRLTVRPPSWRPDLTSPADLVEEVARLGGYDTIPSVLPVAPPGRGLTESQRARRR
                     VGQALAHAGYVEAPSYPFVGESDWAALGLSAGDPRRRALRLHNPLSDEAPALRTTLLP
                     GLLTTLRRNLGRGAADVALFEQGLVFLPRPGAPSAPARPGVAHRPSDEELAALEAVLP
                     DQPRHVAAVAVGQADRAGWWGPGRAQTWADAVAAARLVVDAAGVPATDLRVRAAALAP
                     WHPGRCAELLLTVGGTERVLGHAGELHPRVVANLGLPERTVAMEVDLDLLAAAAGGPV
                     QVGSVSTFPVATQDVALVVDASVAAADVEAALREGAGELLEDLRLFDVYTGEQVEPGH
                     KSLAFALRFRAPDRTLTVEEASAARDAAVAEATRRTAAALRTA"
     gene            complement(15649..16758)
                     /gene="pheS"
                     /locus_tag="VLC50_06290"
     CDS             complement(15649..16758)
                     /gene="pheS"
                     /locus_tag="VLC50_06290"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020552010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HSF27112.1"
                     /translation="MPAPIPEPDPVDVTSLRADHVERVRDEALKAIAAAGDLEALKAV
                     RLEHAGDRSPLALANREIGSLPPPERAGAGQRVGTARRAVAEALTARQGELEAARDAR
                     VLVDEAVDVTLPWDRRPVGARHPLTTLQERIGEVFVAMGWEVAEGPEVEAEWFNFDAL
                     NIAPDHPARSESDTFFVESPDSGVVLRTHTSPVQARTMLTRTPPIYVVCPGRTFRTDE
                     LDATHTPVFHQVEGLAVDEGLTMAHLKGTLDHFAQAMFGEGLTTRLRPSYFPFTEPSA
                     ELDMRCFVCRGASVGNPAAPCRTCSSEGWIELGGCGMVNPRVLRACGVDPDRYSGFAF
                     GMGIDRTLMFRHGVTDMHDIVEGDVRFTLPFGTEI"
     gene            complement(16798..17874)
                     /locus_tag="VLC50_06295"
     CDS             complement(16798..17874)
                     /locus_tag="VLC50_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014157246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSF27113.1"
                     /translation="MDGYDVASTGPVRPDELPDGLLVADAAGLVVLVNRAAERILLAG
                     RDDLLGKSLDDALPLLDLDGRLWWDCQRPYDGLATRTGHPERNLVLGDGREVLVTARY
                     VRAVACGPVQRVVVGLRDTRARAREETSRAELISTVAHELRSPLTSVKGFTATLLAKW
                     ERFSDEQKRLMLETVNADADRVTRLITELLDVARIDSGRLEIHRQMVDLPAAVHRHVA
                     GHLANGEAPERFVVHVSEPLPELWADADKVDQVLANLIENALRHGEGTVTVVVEAAGR
                     AAEGVVVTVMDEGAGIPEDLHQRVFTRFWRSGKRGGTGLGLYIVRGLVEAHGGSIEIG
                     HSASGGARFRFTLPAGTPDFLSAS"
     gene            complement(17885..18724)
                     /locus_tag="VLC50_06300"
     CDS             complement(17885..18724)
                     /locus_tag="VLC50_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018891441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HSF27114.1"
                     /translation="MASPELTSTRSPRVQAARRLAKRAFRAADRRFIAEGPQAVREAV
                     ADPDRVVEIFVTAEAAARYEAIVSAATDAGLPVQRVSGEVMGALAQTVTPQGIAAVCT
                     FLDVPLDVVVAARPRLVAVLAHVRDPGNAGTVIRCADAAGADAVVLTAASVDVYNSKC
                     VRASAGSVFHLPVVVGLSAEQATAALRTAGCAVLAADGAGSRDLDDVIDSGELTRPTA
                     WLFGNEAWGLPDELLGLADEVVRVPIHGAAESLNLATAAAVCLYASARAQRAKGGSAA
                     RSG"
     gene            complement(18767..19141)
                     /gene="rplT"
                     /locus_tag="VLC50_06305"
     CDS             complement(18767..19141)
                     /gene="rplT"
                     /locus_tag="VLC50_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020577506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HSF27115.1"
                     /translation="MARVKRAVNAQKKRRVVLEQASGYRGQRSRLYRKAKEQVTHSLV
                     YAYRDRKRRKGDFRQLWIQRINAAARANGMTYNRFVQGLKAAGVEVDRRVLADLAVTD
                     AKAFAGLVEVARAALPSTTTAA"
     gene            complement(19212..19406)
                     /gene="rpmI"
                     /locus_tag="VLC50_06310"
     CDS             complement(19212..19406)
                     /gene="rpmI"
                     /locus_tag="VLC50_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013838486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HSF27116.1"
                     /translation="MPKNKTHSGAKKRFRLTGTGKVMRERANRRHLFESKPSTRTRRL
                     ALDVETAGSDVKKIKRLLGR"
     gene            complement(19520..20152)
                     /gene="infC"
                     /locus_tag="VLC50_06315"
     CDS             complement(19520..20152)
                     /gene="infC"
                     /locus_tag="VLC50_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013004920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HSF27117.1"
                     /translation="MSVEPRINDRIRVPEVRLVGPNGEQVGIVAIGDALRLAQEADLD
                     LVEVAPDARPPVAKLMDFGKFKYESAMKARESRKNQVNTVIKEMKLRPKIDPHDYETK
                     KGHVVRFLGQGDKVKITIMFRGREQSRPELGFRLLQRLADDVADLGFVETTPKQDGRN
                     MTMVLGPHKKKAEARAEREAAKVQRAAGAVAPQGRSPEDAQSQSGAGQAS"
     gene            20440..20799
                     /locus_tag="VLC50_06320"
     CDS             20440..20799
                     /locus_tag="VLC50_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015794072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1844 domain-containing protein"
                     /protein_id="HSF27118.1"
                     /translation="MPAETSDALDAGAAARDIAEVPAVEVITTAAVHLMSAAAVKCGL
                     AEDAEAAGHLDLDEARKLIIALAALISATGPYLGVHAEPLRDGLRSLQLAFREASPFP
                     DPPGHGPGERWTGPVLP"
     gene            complement(20796..21599)
                     /locus_tag="VLC50_06325"
     CDS             complement(20796..21599)
                     /locus_tag="VLC50_06325"
                     /inference="COORDINATES: protein motif:HMM:NF014193.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SURF1 family protein"
                     /protein_id="HSF27119.1"
                     /translation="MHRWLLSPRWLGLHAVLVLALVLTGVLGWWQLDRARAQHTASQQ
                     HSAATAAAPVPLTTLLAGATQVGPGQAGRAVTLTGHYDPAHQLLVPGRPLHGQPGYLV
                     VAPLVSDSGRAVMVDRGWLPAGEDGRPPAVPAPPQGTLSVTGWLSGPDPMPERAARAE
                     PSGTTPQVMAVNAAVLVNVVPYPVVDGYVHRASAEPGAPGPGAELTAVPRPVQGASGT
                     WPLQNLFYALQWWVFGAAAVWLWLAMVRHEYDDRRRELDARVQALAARR"
     gene            complement(21614..21946)
                     /locus_tag="VLC50_06330"
     CDS             complement(21614..21946)
                     /locus_tag="VLC50_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020575772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3817 domain-containing protein"
                     /protein_id="HSF27120.1"
                     /translation="MTSTASMRTTKSLTRYRVMAWITGVMLLVLVLVAMPLKYAADST
                     TLIAVVGPLHGFLYILYVLSVIDLATRRRWALVRTLLVMLAGVVPFATFYAEHRVVVG
                     ERAAIEAG"
     gene            complement(21956..22780)
                     /locus_tag="VLC50_06335"
     CDS             complement(21956..22780)
                     /locus_tag="VLC50_06335"
                     /inference="COORDINATES: protein motif:HMM:NF018837.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SseB family protein"
                     /protein_id="HSF27121.1"
                     /translation="MTGADSAGVPWGGRTLPTPGFTGDHGLADPTLEAALQELAQAGS
                     DESAVLAAHVRVHAALAQARLLVPVVAVLDTVSTGVDGLHADKSTDMALVTLQARDGA
                     RAVPAFTALDRLSAWRPDARPVPAEARRVALSVVAEGATRVVLDPAGPVTYVLERAPL
                     WALAQGRAWLGPAEDPAVREAVADALAPADVVRAAWVRTTAPSGLRLTLALPPGLARD
                     TLTRLVTEVSHRLGDDQRVADRVDGVSIVLRGDGPAAATEPGRPLTLVGRGREALG"
     gene            complement(22875..24023)
                     /locus_tag="VLC50_06340"
     CDS             complement(22875..24023)
                     /locus_tag="VLC50_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ring-opening amidohydrolase"
                     /protein_id="HSF27122.1"
                     /translation="MPQAIEVRKVPLHGVSDASELEKLIDAGVLQADRVVAVIGKTEG
                     NGGVNDYTRILADRAFREVLVAKGARSMEEVKQVPIVWSGGTDGVISPHASIFATTDV
                     AEGDPSRGEPRLTVGFAMSERLLPEDIGRVGMIEKVAAGVKVAMERAGITDPADVHYV
                     QTKTPLLTIDSIRDAHARGKTVWTTDTLKSMELSNGTAGLGIAVALGEIAMPTDADVL
                     TNFDLFSSVASCSSGVELDRAQIVVVGNARGVGGRYRIGHSVMRDALDADGIWAAMRN
                     AGLDLPERPHWTDIKGRLVNVFLKCEASKDGMVRGRRNAMLDDSDVSWHRQIKGTVGG
                     VTAAVTGDPAVFVSIAADHQGPEGGGPVAAIIDLGPAPATGYVPPAQD"
     gene            complement(24056..24991)
                     /gene="arcC"
                     /locus_tag="VLC50_06345"
     CDS             complement(24056..24991)
                     /gene="arcC"
                     /locus_tag="VLC50_06345"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011754966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019546 - arginine deiminase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HSF27123.1"
                     /translation="MRALIALGGNAMTGPEGGARAQDQIDAVTEAVEHIADLVASGVD
                     VVLTHGNGPQVGNLLVKNELAAAVVPPVPLDWCGAQTQGTIGFIVIDALDAALARRGL
                     ARRTAALVTRAVVDGDDPGFTTPTKPVGRHLPAAEAQAFIDAGQVWSDRGAKGWRRVV
                     ASPEPLEIADAPAVEALVAAGFIVVANGGGGIPVVRDPDGTLRGVEAVIDKDLGAALL
                     ARTVHADVLIIATDVEAAVLSWGTPQARALGQVTVSEMRGHAAEGHFASGSMGPKVDA
                     ACRFIEQGGSRAVITSLDRIADAVAGQAGTIIVPD"
     gene            complement(25122..>25641)
                     /locus_tag="VLC50_06350"
     CDS             complement(25122..>25641)
                     /locus_tag="VLC50_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020015680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HSF27124.1"
                     /translation="QVPRAQGNHHPSIVPYGLFRCRDGAVQIAVGSEGLWRKLCAGFG
                     LDPAAEGMATNAERVEHREQVLAAVEAAFASYDAADLLPRLDELGIPAGKVRTLDEVY
                     AWDQTRSQGLVVEVDHPVLGPVELPGPPLRFFADGDDGGLREVTRREHSAPPALGAHN
                     DSVRAWLDALEA"
BASE COUNT         3464 a         9363 c         9423 g         3391 t
ORIGIN      
        1 aaggaatttc cgtcagaacc aggcacacca ggcacaacac ctgacacacc cagcccccga
       61 cggggatttg gcattgacta ttagcacgct gttgagttct caagcatcgg acgcacacca
      121 caaccccagc cacacgggcc aaagtcacgg gaccgccgcc cgcggtggcc accgaatcgg
      181 tgtcccttgt tgctccctgc cgggcgacaa agagaactgt aggcacgggc cctgacggag
      241 tcaaatcggc ccgagatcgc ccggtgcgca ccgccccgcg gcgcgtggcg ctgggtgcgt
      301 caggaggtca gctcgaccgc ggcgagcgcc cgccggccgc gtcggagcac catccagcgc
      361 cctgccagca ggtccgccgc cgtcgggacg tagtcgccgt cgtcaacctt gcggttgttg
      421 aggtaggcgc ccccctcggc caccgtccgg cgcgccgccg acttggagct gaccagcccc
      481 gaggaggcga gcaggtcgac gtaggccggc acgggggccg agacgctgac ccggggcagc
      541 tcggccagcg agtcggccag cgtgcgctcg tcgagcgagg ccagctcacc ccgcccgaac
      601 agcgcctggc tcgcctcgac cacggctgcc atcgccttct cgccgtggac cagcgcggtg
      661 atctcggcgg ccagcgcccg ctgggcctcc ctggccgccg ggcgggactc caccgccgcc
      721 gcgagcgcct cgatctcctc gcgcggccgg aagctgaaca ccttgaggta gttggcgacg
      781 tcgcggtcgt cggtgttgag ccagaactgg tagaaggcgt agggcgaggt catgtccggg
      841 tcgagccaga ccgagccgga ggccgtcttg ccgaacttcg tgccgtcggc cttggtgacc
      901 agcggcgtgg ccagcgcgtg cgccgagacg ccctcgaccc ggtggatgag gtcgagcccc
      961 gccgtgaggt tgccccactg gtcgctgccg ccggtctgca gcagacagcc gtggcggcgg
     1021 tagagctcga ggaagtccat gccctgcagc acctggtagc tgaactccgt gtaggagatc
     1081 ccagcctcgc tgttgaggcg ggcgctcacc gcgtccttgg tgatcatctt gttgacccgg
     1141 aagtgcttgc cgatgtcgcg cagcaggtcg atcgccgaca gctggccgat ccagtcgagg
     1201 ttgttgacca tgacggcggc gttgggaccc tcgaacgtca ggaacggctg gatctgtgca
     1261 cgcaggcgcc ccacccaggt ggccaccagc tcccgtgggt tgagcgtgcg ctcggtgctg
     1321 cgcccgccgg ggtcgccgat gagcccggtc gcgccgccga cgagggcgag cggccggtgc
     1381 ccggcgagct gcaggcgccg cagcgtgagg atctgcacga ggttgccgat gtgcaggctg
     1441 ggggcggtgg ggtcgaagcc gcagtagtag gtgagcatcc cctggtcgag cgcctcgcgc
     1501 agcgcgccca gatcggtgct ctgcacgatc aggccacgcc actgcaggtc gtcgagaagc
     1561 tcgttggcgg gggtcacggt agggctgcct ctctcggatg ggacggtgcg ggcccctagc
     1621 ctgcccgacc gcgatctggg cccgcgacgg tgcccggcgc gggccggcgg tggcgaggtg
     1681 catgggcacg gtagggcgag acggtcggct cggcttcgag ccagaagcgc cagggccgtt
     1741 cggccccgtc gccgccgacc ccgacgcgtg gtccctggca gacgcccgag gtggggactt
     1801 gcccgcgacg gatgagcagc ggcgagccgg gagtgacgac gtcgagcccg ttccacgtgc
     1861 cgtccaagcc gagagccgag gccagccggg ccgggccgcg ggccaggtcg cgggccacgc
     1921 gggcggccgg ccggcgcgcc cgggccagct cctcccccac gacgacctcc ccgccgcgca
     1981 gcagcacggc cgaggcccag ccctcctcgc ggcagaccag gttgacgcac cagtgcatgc
     2041 catagctgaa gtagacgtag cagtgcccgg gcgggccgaa catcaccgcg ttgcgcttgg
     2101 tgcgcccgcg gaaggcgtgg gagcccgggt cgtgccccga cccgtcgtag gcctcgacct
     2161 cggtcagccg cacgctgacc atgcccgcct gggtgtgcga ggtgatcacc gcgccgagca
     2221 ggtctggggc gacctcgagc accgggcggt cgaagaacgc ccgggccagc acatcggcgt
     2281 acggcgaaac tccgcgcgac cccccgtcgt aggggccggc gccgtctcga ggccgagcgt
     2341 tcaggcgacg cctccccgct gggcggccgg cgccggcacc ggccgcacct cggcccacgc
     2401 cgcgtggtcg gcgaccgcct gcgtcagcgc cgcgagctgc tcggccaccc ggtccggcga
     2461 ggtgccgccg tgggtggcgc gcgcctgcag cgctccgcgc acggtgagga tctcgcggac
     2521 ggcgggggtg agccgggcat cgacgtcggc gagctcggcg tcggccagct cccacagctc
     2581 gacgccacgg gccacgcagc ggcgcacgag cgcaccggcg atctcgtggg cgtcgcggaa
     2641 caccacgcct tggcggacca gccactcggc gacatcggtg gccaacgtga acccctgcgg
     2701 cgcgcactcg gccagccggt cggtgcgcac cgtcatcgtc gcgatcgttc cggccatcgc
     2761 gggcagcacg aggagcagct gctcgaccgc gtcgaagacc ggctccttgt cctcctgcag
     2821 gtcgcggtcg taggccagcg gcagcccctt gagggtcgtg aggaagccgg tgaggtgccc
     2881 gacgaggcgc ccgctcttgc cccgggccag ctcggccacg tcggggttct tcttctgcgg
     2941 catgatcgag gagccggtgg cccaggcgtc gtcgagctcg acccagccga actcggtcgt
     3001 ggtccacagg cacagctcct cgcccagccg ggacaggtgc acgcccagca aggcggcggc
     3061 gaagaggaac tcggccgcga agtcgcggtc ggagaccgcg tcgatcgagt tgtcggccgg
     3121 ccggtcgaag ccgagctcgg ccgcgacggt gaccgggtcc actgcgagcg acgacccggc
     3181 gagcgcgcca gcgcccagcg ggcagaccgc cgcccgcttg tcccagtcgg ccagccgctc
     3241 ggcgtcgcgc ttgaacgcct gcacgtgggc gagcagctgg tgggcgaaca ggatcggctg
     3301 agcgtgctgc aggtgggtca tgcccggcgc cggcgcgttg cggtgggtcg cggcctgggt
     3361 gatgagtgcg gtctcgaggc cggccagcgc gccgaccacg tcgcgcacgg cctcgcgcag
     3421 gtagagccgc aggtcggtcg cgatctggtc gttgcgcgaa cgcccggcgc gcagcttgcc
     3481 cccgagcgcg ccgagccgct cgatcagccc gcgttcgagc gcggaatgca cgtcctcgtc
     3541 ggcctctgtg gccacgaacg tgcccgtgcg tacggcggcg gcgaggtcac cgagcgcctc
     3601 gagcatccgg tcgagctcgt cgtcggtgag cagcccggcc cggtgcagca cccgggcgtg
     3661 cgaccgcgag gcgagcaggt cgtagggcgc cagccgccag tcgaagtggg tgctcttgct
     3721 cagggcggcg agggcgccgg ccggcccacc gctgaaccgt ccgccccaga gagcaccgtc
     3781 gcgggtgtga ttggtgtggt cggccatcag tcggtgcccg tctccatggc ggcgttgtct
     3841 agcgcggact cctcgtcacc ggcaaccgtg gggttgtcac tgatccggtc ggcgccgccg
     3901 gcaggcagcg caccgaacag tgtgccgtgc tcgacgagca gctgcccctg gtccaacggc
     3961 tgggcggcgt aggcctcgag cagcgcccgc gagtcggcga tgtcgaggtt gcgcatggtc
     4021 agctgcccga tccggtcggt cggcccgaac gcggcgttgt cggtgcgttc catcgacagc
     4081 ttgtgcgggt ggtaggagaa gccgggcccg ctggtgcgca gcaccgtgta gtcctcgccg
     4141 cgacgcaggc gcagggtgac ctcgccggtg acgacggagg cgatccagcg ctggatcgac
     4201 tcgcgcagca tcagcgactg cgggtcgagc cagcgtccct cgtagagcag ccgacccagc
     4261 cgtcggccct gcgtgtgata gctggcgacc gtgtcctcgt tgtggatggc gttgagcagc
     4321 cgctcgtaga ccgcccacag cagcgccatg cccggcgcct cgtagatccc gcggctcttg
     4381 gcctcgatga tccggttctc gatctggtcg gacatgccga ggccgtgccg tccgccgatg
     4441 gcgttggcct catgcaccag cgcgaccggg tcgtcgtacg tggtgccgtt gatcgccacg
     4501 ggccggccgt gctcgaaccg gaccgtgacg tcttcgggct cgatgaccac ggcggggtcc
     4561 cagaaccgca cccccatgat cgggtcgacc gtctccagcg agacgtcgag gctctccagg
     4621 gtcttcgcct cgtgcgtcgc gccccagatg ttggcgtcgg tggagtaggc cttctcggcg
     4681 ctgtcccggt agggcaggtc gtgctcgacg agccaccggc tcatctcggc ccggccgccg
     4741 agctggctga cgaaggcctt gtccagccac ggcttgtaga tgcgcagcga cgggttggcc
     4801 agcaggccgt agcgatagaa gcgctcgatg tcgttgccct tgaacgtcga gccgtcgccc
     4861 cagatgtcga cgccgtcggc ccgcatggca cggaccagca tcgtgccggt caccgcgcgg
     4921 cccagcgggg tggtgttgaa gtaggcgcgc ccgccggagc ggatgtggaa cgccccgcag
     4981 gccagggcgg cgagaccctc ttcgaccagc ggtgcccggc agtcgacgac gcgcgccagc
     5041 tcggcgccgt actgcagggc acgggccgga acaccgtcga cgtcggcctc gtcgtactgc
     5101 ccgatgtcgg cggtgtaggt gcacgggacc gcaccgttct cgcgcatcca ggccaccgcc
     5161 accgaggtgt cgagcccgcc ggagaaggcg atgccgacgc gctcgccgac gggcagcgag
     5221 gtgaggacct tgctcacggt gtgttcctcc gcgtggtgtg agagtggatc tggggctggg
     5281 gggcggactc gtccggggcg gcgtctccgc cctcggcgag ggccagcagc tcggtggcca
     5341 gtgcggcacc gtcgggacca cggcagatca gcaggacggt gtcgtcgccg gcgatggtgc
     5401 cgagcaccgc ctcgaggtcg gcccgatcga tcgcggaggc gaggaactgt gccgcccccg
     5461 gcggggtgcg caggacgacg aggttggccg aggcctgcgc cgagacgagc agctcgccgc
     5521 acagccgggc cagccggccg tcgtcgccgg cctgcccgcc cggacgcggg gtgcggtcgc
     5581 cgccctcgcc gggcaccgcg tagacgagcg gaccggcacc ggcccgcacc ttgaccgccc
     5641 cgagctcgtc gaggtcgcgc gagagcgtgg cctgggtgac ctcgatcccg tcctcggcca
     5701 gcaaccgggc cagctcaccc tgcgagcgga cctcgtgacg ggccaggacg tcggcgatgc
     5761 gttgacgtcg ggcggcgggc gtcgccggga cccgcggtga cgttggccgc accggcgccc
     5821 tcgtcgtcgg ggcaccggtc atgaccgctc cagcagccag gccagcagcg ccttctgggc
     5881 gtgcagccgg ttctcggcct cgtcccagac gacgctggcc gggccgtcga tgaccgaggc
     5941 cgagatctcc ttgccccggt aggcgggcag gcagtgcagc acgatcgcgt cgggggcggc
     6001 gcggtcgagc gcggcttcat cgagcgcata gggcaggaac ggtgcggccc ggtcggtgcc
     6061 gtcaccctcc tgacccatcg acacccaggt gtcggtggcc agcacgtcgg cgccctcgac
     6121 ggcggcgtgc gggtcgagca cgtaggccgc cgacccgccg gtctcggcgg cgatcttctg
     6181 ggcccgggcc agcaccgcgg agtcgggcac gaagccctcc ggtgagccga cccgcacgtg
     6241 catgccggcc agcgcgccgc cgagcaggta ggagtgggcc atgttgttgg cgccgtcgcc
     6301 gaggtaggtc agggtcagcc cggccagccg gcccttgtgc tcgcgcacgg tctgcaggtc
     6361 ggccaggatc tggcacgggt ggaactggtc ggtcagcgcg ttgaccaccg gcacccgcga
     6421 caccgcggcc atcgcctcga tgcgttcctg gccgaacgtg cgccacacga tggccaccgc
     6481 ctgccggtcg agcacccggg tggtgtcctc gatcggctcg ccccgcccga gctggctcga
     6541 ctgcgcgtcg atgaccagcg ggtagccgcc cagctcggcg atgcccaccg agaacgagac
     6601 ccgggtgcgg gtggagtgct tgtcgaagag caccgccacc gtgcgcgggc cctcgagcgg
     6661 gcggaacccg aaccggtcgg ccttcatcgc atcggccagg tcgagcacct gcgcctgctc
     6721 ggccggcgtg aggtcgtcgt cgcgcaggaa gtggcggggg gcactcatga cacgtctcct
     6781 tgggtacctg cggagccggc gcggccggca ggggcagcgg cgtcgaggat ggccgggagg
     6841 tcggcgacga acgcgtcgac ctgctcggcg gtgagcacca gcggcggggc cagccgcaac
     6901 gcctgcgggg tcacgtcgtt gacgacgtag ccggcggcca tcgcctcggc cgcgacccgg
     6961 gcggccaccg gcgcggtcag cgtgacggcc agcagcaagc cctgcccgcg caccccgtcg
     7021 accagcgggt gcccgagcgc ggcaatccgc tcacgcagcc gttggcccac ctcgcgcacg
     7081 tgggcgagca ggccgtcgcg ctcgatcacc tgcagcacgg ccagggctgc ggccgcggcc
     7141 accgggttgc ccccgaacgt cgttccgtgc tggcccggac cgagcaggct cgcggccggc
     7201 ccgagggcga ccgtcgcgcc gatcgggagg ccaccaccga ggcccttggc cagcgtgatg
     7261 acgtcgggtc ggaccccgtg ccgggtgtgg gcgaaccact cccccgtgcg gccgatgccg
     7321 gtctgcacct cgtcgagcac cagcaacgcg ccctggctgg cggtgatctc ccgagccgcg
     7381 gccagataac catcaggggg tacgacgacc ccggcctcgc cctggatcgg ctcgaggaac
     7441 accgcggcgg tgtcaccgtc gacggcggcg gccagcgcag ccgtgtcgcc gtacggcacg
     7501 aacgtgacct cgccgggcag cggggcgaac ggctcgcggt aggcgggctt ccaggtcagc
     7561 gcgagcgcac ccatgctgcg gccgtggaac gcgtgctcgg ccgccaccag cctggtgcgt
     7621 ccggtgcgcc gggtcagctt gaaggcggcc tcgttggcct cggtgccgga gttggtgaag
     7681 aacactcggc cgtcaccgcg ggcgtcgagc agctcgagca gccgctcggc cagcgcgacc
     7741 tgcggacggg tcgcgaagta gttggacacg tgcccgaggg tggccagctg gctggtcacc
     7801 gccgccacca cgagcgggtg ggcgtggccg agcgcgttga ccgcgatgcc gccgagcagg
     7861 tcgaggtagc ggcgcccgtc ggcgtcccag acccaggcgc cctcgccgcg cacgagcacc
     7921 cgcttggggg tgccgaacgt gcccatcagc gcgtcgccgt agcgcttggt ccactcgtcg
     7981 ttgccgccgc ccgccgtcag ctcggtcagg gggtcgatcg cggtcatgcc accacctcgg
     8041 tcccgactcc ggagtctgtg aagacctcga gcagcagggc gtgcggcacc cggccgtcga
     8101 ggacgtgggc cttgggcact ccgccgcgta cggcgcgcag gcaggcctcc atcttgggca
     8161 ccatgcccga gctgagggtc ggcagcatgt cctccagctc gccggcgtgc aggctgctga
     8221 tcagctcggc cggctcaccg cccgcgccgt caccgggcca cgaggagtag agcccctcga
     8281 cgtcggtgag cacgacgagc ttctccgcgc ccagggccac cgccagcgcg gcggcggcgg
     8341 tgtcggcgtt gacgttgtag acctggccgt cctcgccgcg ggcgatgctc gagaccacgg
     8401 ggatccgccc gtcggcgacc aggccctgca ccactccggg ctcgacgtgc acgacgtcgc
     8461 cgaccaggcc gacgtcgacg agctcgccgt cgaccagcgc cccgcggcgc tcggcggtga
     8521 acagccgggc gtcctcaccg gagaccccga ccgcgaacgg gccgtgctcg ttgaccagcc
     8581 cgaccacgtt gcgctgcacc tgcccgacca gcaccatccg tacgacgtcc atcgcctccg
     8641 gcgtggtgac ccgcaggccg ccgcggaact cggtgtcgat gccgagccgg tcgaggtggg
     8701 cggtgatctg cgggccgccg ccgtgcacga tcaccgggcg cagcccgacg taccagagga
     8761 acatcacgtc ctcggcgaag gatctgcgca gctcctcgtc ggccatcgcg tggccgccgt
     8821 acttgatcac cgcgaccttg ccgtggaagc gctgcagcca gggcagcgcc tcgaccaggg
     8881 tggccgcctt ctcgcgggcg gcggcgtggc gggtgctcat gaggagtagg ccgagttctc
     8941 gtggacgtag tcggcggtca ggtcggtggt ccagatcgac gcggtggtgt cgccggcgcc
     9001 caggtcgatg gtcaccgtga cggcccgggc cgacatgtcc accagcgacc ggtcggctcc
     9061 cactcccccg tcgcggcaca cccagacgcc gttgatcgcc acggccacgt gctcaggctc
     9121 gaaccgggcc ttggtggtgc cgagcgcgga gagcacccgg ccccagttgg ggtcctcgcc
     9181 gtgcaccgca cacttgaaca gcgcgctgcg cgcgaccgcc cggccgacct cgagcgcgtc
     9241 ggcctcgtcg gcggcccgga ccacctcgac ggtgatctcc ttgctggcgc cctcggcgtc
     9301 ggcgacgagc tggtgggcca ggtcgagaca gacggcctcg acggcctcgg tgagctcggc
     9361 ccggctggga ctgaccccgg aggctccgga ggccagcagc agcacggtgt cgttggtgga
     9421 catgcagccg tcggcgtcga cccggtcgaa gctgtgccgg gtgccctcgc gcagggcggc
     9481 gtcgaggtcg gccggctcgg cgaccgcgtc ggtggtcagt acgaccagca tggtggccag
     9541 cgcgggcgcg agcatccccg cgcccttggc catgccggcg acggtgaagc cggggccggc
     9601 gtacgacgcc tgcttgggca ccgtgtcggt cgtcatgatc gcccgggccg ccgcctcgcc
     9661 gccgtcgtcg gacagctcgc cgaccgcgcg ctcgaccccg gccagcaacg cgtccatcgg
     9721 aagccgctca ccgatcagcc cggtggagca gaccgccaag tcaccggccg agagccccag
     9781 cagctcggcg gcccgctcgg cggtccggtg ggtgtcctgg aagcctgccg cgccggtgca
     9841 ggcgttggcg ccgccggagt tgaggacgac cgcgtcgacc cgtccgtcgc tgaccacctg
     9901 gcgcgaccac atcaccggcg cggccttgac ccggttggag gtgaacacgc ccgcggcgtc
     9961 gtggcgcggg ccgtcattga cgaccagcgc gacgtcgggg tcgccgctgt gcttgaggcc
    10021 ggccgccacg ccggtggccc gaaaaccctg gggcagggtc acgctcacgg cgccaccccc
    10081 gtcacgggca ggcccagcgt ctcgggcagg ccgagcgcca ggttggcgca ctgcacggcc
    10141 gcgccggcgg tgcccttggt caggttgtcg atcgcggcga cggcgatgag ccgcccgacc
    10201 cgcgcgtcga cggtgacctg cacgtgcacg gtgttggacc cgagcgtgcc cgccgtcgtc
    10261 ggccactgcc cctcgggcag caggtggacg aacggctcgt cggcgtaggc ccgctcgtag
    10321 gcaacgcgca ccgccgcggg atcgacaccg gcagccagcc gcgcggtgca ggtggccagg
    10381 atgcctcgcg gcatcggcgc cagcgtgggc gtgaacgaga ccgtcaccgg ggcgccggcg
    10441 cagccgctga ggttctgctc gatctcgggg gtgtgccggt gggtgccacc gaccgcgtag
    10501 gggctcatcg cgcccatcac ctcgctgccg agcaggtgcg gcttgagcga gcggccggca
    10561 cccgaggtgc ccgaggcggc gacgacgacc acgtcgtcgg gctcggcgag gtgctcggtg
    10621 aaggccggcg ccagcgccag cgtcaccgag gtgggatagc agccgggtac ggcgatccgg
    10681 cgtgcgccgg cgaggcgagc ccgggcgccg ggcagctcgg gcaggccgta gggccaggtg
    10741 ccggcgtgct cggtgccgta gaactgccgc cacgccgcgg ggtcggcgag ccggaagtcg
    10801 gccccgcagt cgatgacgac cacgtcctcg ggcagctggg cggccaccgc ggcggacgcg
    10861 ccgtggggca gggcgaggaa cacgacatcg tgcccggcca gcacctccgg ggtcgtcggc
    10921 tccagcgtcc ggtcggccag cgtcggcagg tggtggtgca gcgtgccgag ccgggccccg
    10981 gcgttggcgc ccgcggtcag ggccccgacc tcgagctcag ggtgggcgac gagcagccgg
    11041 agcagctcgc cgccggcgta gccgctggcc cctgcgaccg ccgctcgcat gcccatgagt
    11101 gcctcgattc gtcgacctga agaagaacga agcaagacta tacagaaggg tgcatgacct
    11161 tgcacaaccc gggccggccc cgacgtttct cggtaggttc tccgggtttg ctccgtcaaa
    11221 accctgatgt ccgccacggc accgacctgg gaggctggcg ccatgatcac tgttgaaggc
    11281 ctgtcgaaga gctacggcgg ctacctcgcc gtcgatgacg tgacgttcgc gtgccagccg
    11341 ggcgcggtca ccgggttcct cggccccaat ggcgccggga agacgaccac catgcgcatc
    11401 atcgcgggcc tcacgccacc cagccgcggc gacgcccgca tcggcggcgt gcactaccgc
    11461 gacatcccca acccgggcac ccaggtgggc atcctgctcg acgcgtcggc ccagcacgcc
    11521 gggcgcaccg gccgcgaggt gctcaccctc ggcgccgaga cgatgggcct gccccgcgcg
    11581 cgcgtcgacg agatgctcga gctggtcagc ctcaccccca aggaggccgg ccggcgggtg
    11641 cgcaactact cgctgggcat gcgccagcgg ctgggcatcg cgcacgcgct gctgggcgac
    11701 cccaaggtgc tggtgctcga cgagccggct aacgggctcg acccggccgg catccggtgg
    11761 atgcgcgggc tgctgcgcga ctacgccgac cgcggcggca ccgtgctgct ctcctcgcac
    11821 ctgctgcacg aggtcgaggc cgtcgccgac gagctgctcg tgatcggcaa gggccggatc
    11881 gtcgcccgcg gctccaaggc agagctgctg gccggcgccg gcacgctggt gcgagccctg
    11941 gacaacaccc tgctcgccga cgcactggcc cgggccggcc tcgaggcgag cccgggtgcc
    12001 gacggctgcc tgcgcacccc ggccaccccc gagcaggtcg ggcgcgcggc ggccgccgcc
    12061 ggcgtcgtcc tggtcgagct gcgcaccgcc gacgccggcc tcgaagacat gttcctcgag
    12121 ctcaccgccg acacccaacg cgacgagctg accccgacgg gagcctccgc atgagcacca
    12181 ccgcaacgag caccagcacc gccgcaccgg ccatcgacgt acgccagccg tccgtcccca
    12241 tgctccgcct ggtcaaggtc gagctgcgca agctggtgga cacccgcgcc ggcttctggc
    12301 tggccggctc gatcgtgatc atctcggcgc tcatcaccgc cggcatgctc atctgggccg
    12361 agccccagca cctctacttc ggcgacctct tcggggtgat gaacatcccc accggcgtac
    12421 tgctgccggt gctggcgatc ctgctggtca ccagcgaatg gggacagcgc accggtctgg
    12481 tgacgttcac gctcgagccg cgccgctccc gggtcgtggt cgccaagctg gccaccagcc
    12541 tggtcgccgc ggccggcgcg gtggtcgtcg cgctggcctt cggagcggtc ggcgccgtgc
    12601 tcgccggggt cctgcgcgac ggtgccggtg gctgggaaat caccggtgca ggcctgggca
    12661 actcgatcct gctgcaactg ctggcgctgc tcgagggctt cggcttcgcc atgctgatca
    12721 tgaactcggc ggccgcgatc gtgctgttct tcgcactgcc gaccgcctgg atgatcatcg
    12781 cgtcgatcgt accgtggctg cacgagcacg tgcagccgtg ggccgacctg gccaccgcgc
    12841 agaccccgtt ccagggcccc gacctggcct cggcgagcga gtgggcccac ctcggggtgg
    12901 cctcggtcat ctggatcgcg ctgccgctgg ccctgggcat ctggcgcctg ctgcgcgccg
    12961 aggtgaagta ggcggcgatc ctcccctgcc ccttgcgctg atcagtcgcg atcggcggcg
    13021 accccgggca ctgggcaagg cgttctgacc cctggcaagg cgtgcacgcc ttgccagggg
    13081 tcagaacccc tacttggcgt ggtcattcgc gcaggggtgg gggctaggcg gtgcgcagcg
    13141 cggccgccgt acggcgggtg gcctcggcga ccgcggcatc gcgggcggcg ctggcctcct
    13201 cgacggtgag ggtgcggtca ggcgcccgga accgcagcgc gaacgccagc gacttgtgcc
    13261 cgggctcgac ctgctcgccg gtgtagacgt caaagagccg caggtcctcg aggagctcac
    13321 cggcaccctc gcgcaacgcg gcctcgacgt cggccgcggc gacgctggcg tcgacgacga
    13381 gcgcgacgtc ctgggtggcc accgggaagg tcgacaccga gccgacctgc accggcccgc
    13441 cggccgcggc cgcgagcagg tcgaggtcga cctccatcgc cacggttcgc tcgggcaacc
    13501 cgaggttggc caccacccgc gggtgcagct caccggcgtg cccgagcacc cgctcggtgc
    13561 cacccacagt gagcaggagc tcggcgcacc ggccggggtg ccacggggcg agcgccgcgg
    13621 cccgcacgcg caggtcggtt gccggcaccc cggcggcgtc cacgacgagg cgtgcggcgg
    13681 ccaccgcgtc ggcccaggtc tgagcccggc ccgggcccca ccagccggcc cggtcggcct
    13741 gaccgaccgc caccgcggcc acatgccgcg gctggtcggg cagcacagcc tcgagcgcgg
    13801 ccagctcctc gtcgctggga cggtgagcca cgccgggtcg agccggcgcg ctcggggcgc
    13861 ccggccgcgg caggaagacc agaccctgct cgaacagcgc cacgtcggcc gcgccgcggc
    13921 ccaggttgcg ccgcagcgtc gtgagcagcc ccggcagcag tgtggtgcgc agcgccggcg
    13981 cctcgtcgga cagcgggttg tgcagccgca gcgcccgccg tcgcgggtcg ccggcggaca
    14041 gtcccagggc ggcccagtcg ctctcgccga cgaacggata gctcggcgcc tcgacatagc
    14101 cggcatgggc caacgcctgg cccacccgcc ggcgggcccg ctggctctcg gtgagccctc
    14161 gcccgggcgg ggcaaccggc agcacgctcg ggatcgtgtc gtagccgccc aggcgggcga
    14221 cctcctcgac gaggtcagcc gggctggtca ggtcgggacg ccagctcggc gggcgcaccg
    14281 tgagccgctg accgtctcgc tcgacgacgg cgccgaccgc ctccagggcc ccgacgacct
    14341 cgtcgtcggt gtaaggccga cccaccaggc gggtggggtg cgtgatgtcg agctcgatga
    14401 cgaccggctc ccgcggcgtc tgcaccagcg tcacgtcggg ggccagcacc ccgccgccgt
    14461 gctcgacgag cagccgggcg gcccgcagcg ccgccgccag cgccaccgcc gggtcgacgc
    14521 cgcgctcgaa ccggcggctg gcctcgctcg gcaaccggtg ccgtcgggcg gttcgcgcga
    14581 tcgacaccgg gtcgaagtgc gccgcctcga tcacgatctc gctggtccgc tcgccgatct
    14641 cggtggcctg cccacccatc accccggcca gcccgatcgg cccggagccg tcggtgatca
    14701 acaggtcggt cgggtcgagc gcccggtcga cgtcgtcgag cgtgcgcagc cgctcgcctt
    14761 cacgggcacg gcgtacgacg atcgcgccgg tcagccgagc cgcgtcgtag gcatgcagcg
    14821 gctggcccag ctcgagcatg acgtagttgg tcacgtcgac ggccaacgag atggagcgca
    14881 tgccgccggc gcggacccgg cgcgccagcc agtcggggct gggcgcagcc gggtcgaagc
    14941 cgctgacccg caccgcgacg aacgcgtcgc agcctgttgg gtcgtccacg gccaccgggt
    15001 agccgcccgt cgagacgttc gcgccgtcgg tggcgaggtc ggcgggatcc cgccagggca
    15061 cgtcaaaggc ggttgccgcc tcgcgggcca cgccgcgcac cgacaggcag tagccccggt
    15121 cgggggtgac cgcgatgtcg agcacctcgt caccgagccc cagaagctcg gccgcgtcgg
    15181 cacccacggc agcggccggg tcgagcacga ggatcccgct gtggtcctcg ccgatcccga
    15241 gctcgcgcac cgaggcgatc atgccgtcgg agaggtgccc ataggtcttg cgcgccgtga
    15301 tcgggaacgg gccgggcagc acggtgccag gcagcgccac gacgacgtgg tcgccgaccg
    15361 cgaagttgtg cgcgccgcag acgatgccgc gcacctccgg ctcgacctcg gtgccggtgc
    15421 cctccgggcg cacccgcacc tggcaccaac ggatcgtctt gccgttcttc tgcggctcct
    15481 ccacgaagtc gagcacccgg ccgacgacca acggaccgga cacgtcgccg acccgttcga
    15541 ccgtctccac ctcgaggccg gcccgcagca gccgctcggc aacctctcga ccgtcggccg
    15601 tggccggcag gttgacgtag tcccgcaacc aagacagcgc gacgcgcatc agatctccgt
    15661 cccgaaggga agcgtgaagc gcacgtcgcc ctcgacgatg tcgtgcatgt ccgtgacccc
    15721 gtgacggaac atgagtgtgc ggtcgatccc catgccgaac gcgaagccgg agtagcggtc
    15781 gggatcgacg ccgcaggcgc gcaggacgcg ggggttgacc atgccgcagc cacccagctc
    15841 gatccagccc tcgctcgagc aggtgcggca cggtgccgct gggttgccca ccgacgcgcc
    15901 gcggcagacg aagcagcgca tgtcgagctc ggccgacggc tcggtgaacg ggaagtagga
    15961 cggacgcagc cgggtcgtca ggccttcgcc gaacatcgcc tgggcgaagt ggtcgagcgt
    16021 gcccttcagg tgcgccatgg tcagcccctc gtcgacggcg aggccctcga cctggtggaa
    16081 cacaggcgtg tgcgtggcgt cgagctcgtc ggtgcgaaac gtccggccgg ggcacacgac
    16141 gtagatcggc ggggtgcggg tcagcatggt gcgggcctgc accggcgagg tgtgggtgcg
    16201 cagcacgacc ccggagtcgg gcgactcgac gaagaacgtg tccgactccg agcgggccgg
    16261 gtggtcgggg gcgatgttga gggcatcgaa gttgaaccac tcggcctcga cctcaggccc
    16321 ctccgccacc tcccagccca tcgcgacgaa cacctcaccg atccgctcct gcagcgtggt
    16381 gagcgggtgg cgggcgccga ccggccggcg gtcccacggc agggtgacgt cgaccgcctc
    16441 gtcgaccagc acccgcgcgt cgcgggccgc ctcgagctct ccctggcgag cggtcagcgc
    16501 ctcggccacc gcccgacgcg cggtgccgac gcgctggccg gcgccggctc gctccggcgg
    16561 cggcagagag ccgatctcgc ggttggccag agccagcggc gagcggtcac cggcgtgctc
    16621 gagcctgacc gccttgagcg cctcgagatc gcccgcggcc gcgatcgcct tgagcgcctc
    16681 gtcgcggacg cgctcgacgt ggtcggcgcg caggctggtc acgtcgacgg gatcgggctc
    16741 ggggatcggt gcgggcatgc gggcgctccg gcagatgagg taggcggcag cgggagtcta
    16801 cgaggcggac aggaagtcgg gcgtgccggc aggcagcgtg aaccggaacc gggcaccgcc
    16861 cgacgccgag tgcccgatct cgatcgaccc gccgtgcgcc tccacgagcc cgcgcacgat
    16921 gtagaggccg agcccggtgc cgccccgctt gccggagcgc cagaacctcg tgaacacccg
    16981 ctggtgcagg tcctcgggga tgcccgcgcc ctcgtccatc accgtgacga ccacgccttc
    17041 agcggcccgg cccgccgcct ccaccacgac ggtgacggtg ccctcgccgt ggcgcagggc
    17101 gttctcgatg aggttggcca gcacctggtc gaccttgtcg gcatcggccc acagctcggg
    17161 cagaggctcc gagacgtgta cgacgaaccg ctcgggtgcc tcgccgttgg ccaggtgacc
    17221 ggccacgtgc cggtgcaccg cagccggcag gtcgaccatc tggcgatgga tctcgagacg
    17281 ccccgagtcg atgcgggcca cgtcgagcag ctcggtgatc aaccgggtca cccggtcggc
    17341 gtcggcgttg acggtctcga gcatgagccg cttctgctcg tcggagaagc gctcccactt
    17401 ggccagcaac gtcgccgtga accccttgac gctggtcagc gggctgcgca gctcgtgggc
    17461 caccgtggag atgagctcgg cccggctggt ctcctcccgg gcccgagccc gggtgtcgcg
    17521 caacccgacc acgacgcgtt gcacgggccc acacgccacg gcacgcacgt agcgggcggt
    17581 gaccagcacc tcgcgcccgt cgccgagcac gagattgcgc tcggggtgcc cggtgcgggt
    17641 ggcgagaccg tcgtagggac gctggcagtc ccaccagagg cgaccgtcga ggtcgagcag
    17701 gggcagcgcg tcatcgagcg acttgccgag caggtcgtct cgaccagcga gcaggatccg
    17761 ctcggcggcc cggttgacca ggaccaccag gcccgccgcg tccgcgacga gcaggccatc
    17821 gggcagctcg tccgggcgaa ccgggccggt cgacgcgacg tcgtacccgt ccacggcggc
    17881 cagcctagcc agaccgggct gcggagccgc ctttcgcgcg ctgggcccgg gcggaggcat
    17941 agaggcagac cgcggccgcg gtggccaggt tgaggctctc cgccgccccg tggatcggca
    18001 cccggacgac ctcgtcggcc agcccgagca gctcgtcggg cagaccccag gcctcgttgc
    18061 cgaacagcca ggcggtgggc cgggtgagct cacccgagtc gatgacgtcg tcgaggtcgc
    18121 gggaccccgc accgtcggcg gccagcacag cgcagcccgc ggtccgcagc gccgccgtgg
    18181 cctgctcggc cgagaggccc acgacgaccg gcaggtggaa cacgctgccc gccgaggcgc
    18241 gcacgcactt ggagttgtag acgtcgaccg aggcggcggt cagcacgacc gcgtcagcgc
    18301 cggccgcgtc ggcgcaccgg atgacggtgc cggcgttgcc cgggtcacgc acgtgggcca
    18361 gcaccgcgac cagcctgggc cgcgccgcca cgaccacgtc gagcgggacg tcgaggaacg
    18421 tgcacacggc ggcgatgccc tgcggcgtga cggtctgggc cagggcgccc atcacctcgc
    18481 cggagacccg ctgcaccggc agcccggcat cggtcgcggc ggagacgatc gcctcgtagc
    18541 gggcggccgc ctcggcggtc acgaagatct cgaccacccg gtcggggtcg gccaccgcct
    18601 cacggaccgc ttgcgggccc tcggcgatga agcgccggtc ggcggcccga aacgcacgct
    18661 tggcgagccg ccgagccgcc tggacccgcg gggagcgggt cgacgtcagc tcggggctcg
    18721 ccatcgcgtc gtacgtcgtc agcggatgcc tccgcccaga cgtcggtcag gcggccgtcg
    18781 tcgtactggg cagggccgcg cgggccacct cgacgaggcc ggcgaaggcc ttggcgtcgg
    18841 tgacggccag gtcggcgagc acccggcggt cgacctcgac gccggccgcc ttcaggccct
    18901 ggacgaaccg gttgtaggtc atgccgttgg cccgggcggc cgcgttgatc cgctggatcc
    18961 acagctggcg gaagtcgccc ttgcgacgct tgcggtcgcg gtaggcgtag accagcgagt
    19021 gggtgacctg ctccttggcc ttgcggtaga gccgcgaacg ctgcccgcgg tagccgctgg
    19081 cctgctcgag aacgacccga cgcttcttct gggcgttgac tgcccgcttc acgcgtgcca
    19141 cgtggttact cctctaggtc gcggccgagc gcaggctcag gccgctgggg gtggtcgtga
    19201 gtgggccggt ctcagcgacc gagcaacctc ttgatcttct tgacgtcact gcccgccgtc
    19261 tcgacgtcga gcgccagccg gcgcgtgcgg gtcgagggct tgctctcgaa caggtggcgg
    19321 cggttggccc gctcgcgcat caccttgccg gtgccggtga ggcggaagcg cttcttggcc
    19381 ccgctgtggg tcttgttctt cggcatgacg cctctcgtct cttcctgcgc gccgccgctg
    19441 gcggcgcgtg tctgtggtcg ttcggcactg cggtcgtgcg ggactgcggg tctgcccgac
    19501 ccgcggcctg gcgctggcct caggaggcct gccctgcccc gctctgggac tgcgcgtcct
    19561 cgggcgagcg tccctgcggc gcgacggcgc cggcggcgcg ctggaccttc gcggcctccc
    19621 gctcggcccg cgcctcggcc ttcttcttgt gcggccccag caccatggtc atgttgcgcc
    19681 cgtcctgctt gggcgtggtc tcgacgaagc ccaggtccgc cacgtcatcg gccagccgct
    19741 gcagcagccg gaacccgagc tcgggacgag actgctcgcg cccacggaac atgatcgtga
    19801 tcttgacctt gtccccctga ccgaggaacc gcacgacgtg acccttcttg gtctcgtagt
    19861 cgtgcgggtc gatcttgggt cggagcttca tctccttgat gaccgtgttg acctggttct
    19921 tgcgggactc gcgcgccttc atggccgact cgtacttgaa cttgccgaag tccatgagct
    19981 tggccacggg cgggcgagcg tccggagcga cctcgaccag gtcgaggtcg gcctcctggg
    20041 ccagtcggag cgcgtcgccg atggcgacga tgccgacctg ctcgccgttg ggaccgacga
    20101 gccggacctc aggaacgcgg atccggtcgt tgatgcgggg ttcgacgctg atggggcctc
    20161 ctcgagtcgc acagcctgcc cgcgagcacg agaaaggctc ccgtgcaggg agtgcccggg
    20221 agccaacgac gccggccccg cacgaggcgg tgccgacgac cggtcgaacc cgtcggcctc
    20281 cgggatgtcc cggcgggcgt gcgcccgagc ggtcgaggcg ggtgggagct ggctcctctt
    20341 gcacacacct ggggctggcg gcacccatcc gagacggtgg acacccccgg tcacccaggt
    20401 cggtcggtct gagagtgtaa cagcgtgaac gactccccta tgcctgccga gaccagcgac
    20461 gccctcgatg ccggcgccgc cgcccgcgac atcgccgagg tgccggcggt cgaggtgatc
    20521 acgacggccg cggtgcacct gatgagcgcg gccgccgtca agtgcggcct ggccgaggac
    20581 gccgaggccg ccgggcacct cgacctcgac gaggcacgca agctgatcat cgcgttggcc
    20641 gccctgatca gcgcaaccgg gccctacctc ggcgtgcatg ccgagccgct gcgcgacggc
    20701 ctgcgctcgc tgcagctggc cttccgggag gcctcgccct tccccgaccc gcccggccac
    20761 gggccgggcg agcgctggac cgggccggtg ctgccctagc ggcgagccgc cagcgcctgc
    20821 acccgcgcgt cgagctcacg gcggcggtcg tcgtactcgt gccggaccat cgccagccac
    20881 agccagaccg ccgcagcccc gaagacccac cactgcagcg catagaagag gttctgcaag
    20941 ggccaggtgc ccgaggcacc ttggaccggg cggggtacgg cggtcagctc ggcccctggc
    21001 cccggcgcac ccggctcagc cgaggcgcga tggacgtagc cgtcgacgac cgggtagggc
    21061 accacgttga cgagcaccgc cgcattgacc gccatcacct gcggcgtcgt cccggacggc
    21121 tctgcccggg ctgcgcgctc gggcatcggg tcgggtccgg acagccatcc cgtgaccgag
    21181 agcgtgccct gcggaggcgc cgggaccgcc ggggggcgtc cgtcctcacc ggccggcagc
    21241 cagccgcggt cgaccatcac ggccctgccc gagtcgctga ccaggggcgc gacgacgagg
    21301 tagccgggct gaccgtgcaa cggccgaccc ggcacgagca gctggtgggc ggggtcgtag
    21361 tggccggtga gggtgaccgc gcgcccggcc tgcccggggc cgacctgcgt cgcgccggcg
    21421 agcagcgtgg tcagcggcac cggggccgcg gcggtggcgg cgctgtgctg ctgcgacgcc
    21481 gtgtgctggg cgcgcgcccg gtcgagctgc caccagccca gcacgccggt gaggacgagc
    21541 gcgagcacga gcacggcgtg caggccgagc caacggggcg agagcagcca gcggtgcacg
    21601 gccggccggg tgcctagccc gcctcgatcg ccgcgcgctc acccacgacg acgcggtgct
    21661 cggcgtagaa ggtcgcgaac gggacgacgc cggccagcat cacgagcagg gtgcggacca
    21721 gtgcccagcg tcggcgggtg gccaggtcga tgaccgagag gacgtaaagg atgtagagga
    21781 acccgtgcag cggtcccacg acggcgatca gcgtggtgct gtcggcggcg tacttcagcg
    21841 gcatcgccac cagcacgagg acgagcagca tcaccccggt gatccaggcc atgacccggt
    21901 agcgggtcag cgatttcgtc gtacgcatgc ttgccgtcga ggtcacgctg ggagcctaac
    21961 cgagagcctc gcgaccccga ccgaccagcg tcaggggccg ccccggctcg gtcgcggccg
    22021 ccgggccgtc gccgcgcagc acgatcgaga ccccgtcgac acggtcggcc acccgctggt
    22081 cgtcgccgag gcggtgggac acctcggtga ccagccgggt gagggtgtcg cgggccagtc
    22141 cgggcggcag ggccagggtg agccgcagcc cgctcggtgc ggtggtgcgc acccaggccg
    22201 cccgcacgac gtcggcaggg gccagcgcat cggccacggc ctcgcgcacc gccgggtcct
    22261 cggccgggcc cagccaggcc cgcccctgcg cgagtgccca cagcggcgcg cgctccagga
    22321 cgtaggtcac cgggccggct gggtcgagca cgacgcgggt cgcgccctcg gcgacgaccg
    22381 acagggccac ccggcgcgcc tcggccggca ccgggcgcgc gtcgggtcgc cacgcgctca
    22441 gccggtcgag ggcggtgaag gccgggaccg cgcgagcgcc gtctcgggcc tgcagcgtga
    22501 ccagcgccat gtcggtggac ttgtcggcgt gcagcccgtc gacgccggtg ctcaccgtgt
    22561 cgagcaccgc cacgaccggc accaacaggc gggcctgcgc cagcgcggcg tgcacccgca
    22621 cgtgggcggc cagcaccgcg gactcgtcgc tgccggcctg ggcaagctct tgaagcgccg
    22681 cctcgagcgt cgggtcggcc aacccgtggt cgccggtgaa cccgggtgtg ggcagcgtgc
    22741 gccctcccca ggggacgccc gcgctgtcgg cgccagtcac cgggccagcc taggcaggcc
    22801 cagacgatcg cacggcccgg cccccagggg atggggcgcg ggccgtgcga gcagggtggg
    22861 actgcgcagg agcgtcagtc ctgtgccggc gggacgtagc cggtggccgg cgccggaccc
    22921 aggtcgatga tcgcggcgac tgggccgccg ccctcggggc cctggtggtc ggcggcgatg
    22981 gacacgaaga ccgccgggtc gccggtgacc gcggcggtga cgccaccgac ggtgcccttg
    23041 atctggcggt gccagctcac gtccgagtcg tccagcatcg cgttgcgacg accgcggacc
    23101 atcccgtcct tggacgcctc acacttgagg aacacgttga ccagccgacc cttgatgtcc
    23161 gtccagtgcg gacgctccgg caggtcgagc ccggcgttcc tcatcgccgc ccagatgccg
    23221 tcggcgtcca gcgcgtcccg catcaccgag tgcccgatcc ggtagcggcc gccgaccccg
    23281 cgggcgttgc ccaccaccac gatctgcgcc cggtccagct ctacccccga cgagcaggag
    23341 gccaccgagg agaacaggtc gaagttggtc agcacgtccg cgtcggtggg catggcgatc
    23401 tcgcccaacg ccaccgcgat ccccagcccg gcggtcccgt tggacagctc catcgacttg
    23461 agcgtgtcgg tggtccagac cgtcttgccg cgcgcgtgcg cgtcgcggat ggagtcgatg
    23521 gtcagcaacg gggtcttggt ctgcacgtag tgcacgtcgg ccggatcggt gatcccggcc
    23581 cgctccatgg ccaccttcac cccggcggcc accttctcga tcatgccgac ccggccgatg
    23641 tcctcgggca gcagccgctc gctcatcgcg aacccgacgg tcagccgcgg ctcgccccgc
    23701 gacgggtcac cctcggccac atcggtggtc gcaaagatcg acgcgtgcgg gctgatcacc
    23761 ccgtcggtgc cgcccgacca cacgatcggg acctgcttga cctcctccat cgaccgggcg
    23821 cccttggcca ccagcacctc acggaaggcc cggtcggcca ggatgcgggt gtagtcgttg
    23881 accccaccgt tgccctcggt cttgccaatg accgcgacca cgcggtcggc ctgcaagacg
    23941 ccggcgtcga tgagcttctc cagctcggaa gcgtcggaga cgccatgaag ggggacctta
    24001 cggacctcga tggcctgggg cacgtcgtac tcctcgatcg tgtggtggtg atgggtcagt
    24061 cgggaacgat gatggtgccg gcctgcccgg cgaccgcgtc ggcgatgcgg tcgagcgagg
    24121 tgatcacggc ccgggagccg ccctgctcga tgaaccggca cgcggcatca accttgggcc
    24181 ccatgctccc gctcgcgaag tgcccctcgg ccgcatggcc gcgcatctcg ctgacggtga
    24241 cctggcccag cgcccgcgcc tggggggtgc cccagctcag cacggccgcc tcgacgtcgg
    24301 tggcgatgat caggacgtcg gcgtgcacgg tgcgggccag cagcgccgcg ccgaggtcct
    24361 tgtcgatgac cgcctcgaca ccgcgcaggg tgccgtcggg gtcgcggacc accgggatgc
    24421 cgccgccgcc gttggccacg acgatgaacc cggcggccac cagggcctcg acggccgggg
    24481 cgtcggcgat ctcgagcggc tcgggtgagg cgaccacccg gcgccagccc ttggcacccc
    24541 ggtccgacca gacctggccg gcgtcgatga acgcctgcgc ctcggcggcg ggcaggtggc
    24601 gcccgaccgg cttggtgggg gtggtgaagc cggggtcgtc gccgtcgacg acggcgcggg
    24661 tgaccagcgc cgccgtacga cgggcgaggc cgcgccgggc cagggccgcg tcgagggcgt
    24721 cgatcacgat gaacccgatg gtgccctggg tctgcgcgcc gcaccagtcg aggggcaccg
    24781 ggggtacgac ggccgcggcg agctcgttct tgaccaggag gttgcccacc tgagggccgt
    24841 tgccgtgggt cagcaccacg tcgacaccgg aggcgacgag atcggcgatg tgctcgaccg
    24901 cctcggtgac cgcgtcgatc tggtcctgcg cccgggcacc gccctcgggg ccggtcatcg
    24961 cgttgccgcc caaggcgatc agtgcacgca tcccggcacc tcccaatgca gcggcacacg
    25021 tcgcggtccc gcgacgccac cgtgagccta ggaagtgggc tcgcggtccg gcattgtcaa
    25081 gagtcgccca acatccgtcc cgacgatgtg aagttggcgc gctaggcctc gagcgcgtcg
    25141 agccaggccc gcacgctgtc gttgtgcgcg cccagcgccg gcggggccga gtgctcgcgg
    25201 cgggtcacct cgcgcaggcc gccgtcgtcg ccgtcggcga agaagcgcag cggcggcccg
    25261 ggcagctcga ccgggccgag cacggggtgg tcgacctcga cgacgagacc ctgcgaacgg
    25321 gtctggtccc aggcgtagac ctcgtcgagg gtgcggacct tgccggccgg gatgcccagc
    25381 tcgtccaggc gcggcagcag gtcggccgcg tcgtaggacg cgaacgccgc ctcgaccgcg
    25441 gcgaggacct gctcacggtg ctcgacccgc tcggcgttgg tggccatgcc ctcggccgcc
    25501 gggtcgagcc cgaagccggc gcagagcttg cgccagagcc cctcgctgcc gacggcgatc
    25561 tgcacggcac cgtcgcggca gcggaagagc ccgtagggaa cgatcgaggg gtggtggttg
    25621 ccctgcgcgc ggggcacctg g
//
LOCUS       DATIBB010000166        14879 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_1021190, whole genome shotgun sequence.
ACCESSION   DATIBB010000166 DATIBB010000000
VERSION     DATIBB010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 14879)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14879)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14879
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1021190"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..327)
                     /locus_tag="VLC50_06355"
     CDS             complement(<1..327)
                     /locus_tag="VLC50_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012245773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF27125.1"
                     /translation="MADPADRLMPGTHTAANRKANHNGHAGRAVVFVDGVRTPFGKAG
                     PKGIYAETRADDLVIACIRELMRRNPTLPAERVDDVAIAATTQIGDQGLTIGRTAALL
                     SGLPASV"
     gene            complement(394..741)
                     /locus_tag="VLC50_06360"
     CDS             complement(394..741)
                     /locus_tag="VLC50_06360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF27126.1"
                     /translation="MTTTAADQALTALDPLAIDRLPWAPLQGSTGAAYKVLWRSGAMV
                     QTLVHYHEGGWSAGEPHPAHHHVWVISGAATIAGRRLTAGSYVHVPPGLEHPTTDVGP
                     EGATLLVLYLPAA"
     gene            complement(830..1366)
                     /locus_tag="VLC50_06365"
     CDS             complement(830..1366)
                     /locus_tag="VLC50_06365"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HSF27127.1"
                     /translation="MFRRRSQLETVSIDARGVPDLTQLRWPASDYYALHLEAIREDDF
                     PKRVMASWGLIARGVEAMPFLLKMLHSDSADSREDAAGALAWIGTSSPEVVAALLDAL
                     RRSVADEERDSILLGLGGLRAREAVPAVAEILRDPTVDGDTRHTAAEALGQIVRRRFD
                     KQPDPIQAALGWLDAHPT"
     gene            complement(1398..2138)
                     /locus_tag="VLC50_06370"
     CDS             complement(1398..2138)
                     /locus_tag="VLC50_06370"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HSF27128.1"
                     /translation="MRPTPLSRQLVRGLGLVTAALVLPVLAGCSSDSATDASGTAGSE
                     VAVTATDTSCTLSRSELAAGSTTFEVTNSGSKVTEVYVYGEKDGAFTKVISEVENIGP
                     GTSRDMTVDLGGGTYEIACKPGQTGDGIRATVTVAGDQAAGASTGSNSEAAYDRELEL
                     GTDGTSITGLDGESATVGEKIEFKLDNDADGARTLEIKRPDGSVAGEVEIAAGADGEL
                     VVELASPGQWQVIVEGAEPELSAPLDVR"
     gene            complement(2150..2998)
                     /gene="efeU"
                     /locus_tag="VLC50_06375"
     CDS             complement(2150..2998)
                     /gene="efeU"
                     /locus_tag="VLC50_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004931954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter permease EfeU"
                     /protein_id="HSF27129.1"
                     /translation="MLANYLIGLREGLEASLVVSILIAYLVKTGHQRKIPPIWAGVGV
                     AIAMSLGVGALLTFTSRHLSFEAQEAFGGTLSIVAVGFVTWMVFWMRRTARFMKAELQ
                     GRLDSAVAIGTGALVLLAFIAVGREGLETSVFIWAAVQATGSGAAPLAGATLGLLSAA
                     VLGWLFYKGAVRINLAKFFTWTGAALIVVAAGVLAYGVHDLQEAGILPGLHSLAFDVS
                     AQVPPDSWYGTLLKGIFNFSPASTWIEVVVWVGYLVPVGYLFFRPARQRPTTPTTASV
                     PQATGA"
     gene            complement(3053..4294)
                     /locus_tag="VLC50_06380"
     CDS             complement(3053..4294)
                     /locus_tag="VLC50_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004981996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HRDC domain-containing protein"
                     /protein_id="HSF27130.1"
                     /translation="MTAPAVPAPHPAPVPLVEPVEGLPRVVADPTELADAADRLAHGH
                     GPVAIDAERASGYRYGQRAYLVQLRRRGAGTVLVDPVPFTDLSGLAAAADDEWVFHAA
                     SQDLPCLGELGMHPRTIFDTELAARLAGFPRVGLGPLVEHVLGLVLEKGHSAADWSTR
                     PLPEPWLRYAALDVEVLIELRDALETELDRQGKLGWAREEFAAIAAAPPPAPRADPWR
                     RTSGLHRVHKRRQLAAVRALWEIRDRLAAERDMSPSRLLPDSAIIEAAMALPADTAAL
                     LELPGFVGRGAKRYARDWAGAVESARRMPERALPPPTRPAEGPPPARSWRDRDPSAAA
                     RLADARARLAELAGELALPVENLLAPDTVRRLAWAPPQPVEAESVAAFLTEHGARAWQ
                     VRLCTELLVTALTAPTADLRV"
     gene            complement(4329..4958)
                     /locus_tag="VLC50_06385"
     CDS             complement(4329..4958)
                     /locus_tag="VLC50_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019890177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HSF27131.1"
                     /translation="MVERSGRRATTATATAGRPERFSALVVLAAAPARQAVTRRLFAL
                     GAHDVAEATTLAEARTIARAGDPRDLAVVELGLVDGSGLALLDELRTAGWPHGVVITG
                     SDDPSSVRAALSCGVRALLVSRPAPRPVAPLQVLGAKRAAHGLAGLSAREIEVLRLVS
                     DGRTNRDVGEALGLSALTVKSHLARIARKLGTGDRAEMVAMAMRGGLIR"
     gene            complement(5080..5712)
                     /locus_tag="VLC50_06390"
     CDS             complement(5080..5712)
                     /locus_tag="VLC50_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020575746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3000 domain-containing protein"
                     /protein_id="HSF27132.1"
                     /translation="MASGSRGAKRARSASDSVNDPTELFAHALRQLREVTVRAELHIE
                     EAPAPARLAPHAVALTGDVTVDGDDLATGRFVLLHDPAGHDAWAGTWRVVSFVRAELE
                     TDLAADPMLTSVGWAWLTEALEARAAQALVLGGTVTRVSSESFGTMSERPPEAELELR
                     ASWTPAWDGPMPQVAAHLLAWADLLCTAAGLAPLPPGIVSLPNRRATRKA"
     gene            5805..6683
                     /locus_tag="VLC50_06395"
     CDS             5805..6683
                     /locus_tag="VLC50_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015032087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HSF27133.1"
                     /translation="MSLVLALFSSVLWGGADFLGGTLTRRRAVLHVVGASQAVGLLTV
                     VLLALTAGSFGEQGDYLPYAVGAGLAGAVGLLAFYAALAAGSMGVVSPIAALGVVVPV
                     GVGLARGEAPSPVVLAGIAVAVLGVVLASGPELRGRAGAKPLLLAGVAALGFGLALLL
                     IAEGARTSPLMTMVGLRAASVGAVVTVLLVAALRRQLARPVGAGEAEPPPAPPWRRAE
                     VALLVVIGVADVMANVVFALATRGAYVSVVSVLGSLYPVVTVLLAMGVHGERLARVQT
                     IGIATALGGVLLIAGG"
     gene            6706..7773
                     /gene="hemE"
                     /locus_tag="VLC50_06400"
     CDS             6706..7773
                     /gene="hemE"
                     /locus_tag="VLC50_06400"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016815624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HSF27134.1"
                     /translation="MPSPRPDLRDDRPDLSGSAFLRAARREPVPWTPVWFMRQAGRAL
                     PEYRRVREGVPMLEACLRPDLVVEITMQPVRRYGVDAAVLFSDIVLPLRAAGVDLDLV
                     PGTGPVIAAPLRTRRDVTGLPTLEPDHVAPVAAAVRGLVGELGATPLIGFAGAPFTLA
                     SYLIEGGPSKAHVRTRAFMHAEPALWHELLGRLATLSATFLRVQVEAGASAVQLFDSW
                     VGALSAADYREFVLPHSRAVLEGIADLGVPRVHFGVGTGELLGLMGQAGADVVGVDWR
                     VPLDEAVRRIGPGRAVQGNLDPALALCPWSVLHPHVLAVLEAGRAAEGHIFNLGHGVL
                     PETDPDTLTRLVELVHESTAR"
     gene            complement(7811..8845)
                     /locus_tag="VLC50_06405"
     CDS             complement(7811..8845)
                     /locus_tag="VLC50_06405"
                     /inference="COORDINATES: protein motif:HMM:NF025616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HSF27135.1"
                     /translation="MAGTTKPAGRPSRARRGGLLTLLVAAGLALATACSGAVTSSGSS
                     EAAAPVSAGDAGSGGLDAGTAGGATEQGATADRDASAAEGKPPAGAPDAAGVALVPRD
                     LVVTGSLVVRVKDVSDAAARTRSLATTLGGVVSSERIIGGAGEDTPTSRAGGEHPKGA
                     RDSAQLTLRVPTDRADEAMDKLADLGTLLERDLTSEDVTAQVVDVEARVATARASVER
                     VRALLDRADTLGEVVQIESELTRRQADLESLEAQRKRLANLTDLATVNVTLVPPGTTV
                     AAATGGFLGGLRAGWTAFGSAVGVGLTALGAMLPFLVLGALVAIPVRVALRRRARRPA
                     GPPAEDGTPA"
     gene            8931..10409
                     /gene="hemG"
                     /locus_tag="VLC50_06410"
     CDS             8931..10409
                     /gene="hemG"
                     /locus_tag="VLC50_06410"
                     /EC_number="1.3.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020575749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase"
                     /protein_id="HSF27136.1"
                     /translation="MQASDGQGRTGRGGADVVVVGGGIAGLAAAWYLSERAGRRVTVL
                     EASPRVGGKLHASNVAGASVDEGAESLLVRRPEGLALARDLGLGDELVAPATLHASVV
                     RRGRLHPLPRGQVMGVPADLTALARSGLLTVPELARTTLDHLLPRTPMGEDVAIGRQV
                     RARLGAAVVDQLVEPLLGGVYAGHADELSFDMTVPALFAAATRTRSLLRAAAGLRRAS
                     TSAVEGSDTSAQVFASLPGGLARLVDRLVERLAERGVSLLTRTTARELRRTETGWALV
                     VGAASAPRTMSADAVVLATPSAPASRLLAGVAPAAAAELAAIDYASMAIITLAYPRAA
                     FARMPAGSGFLVPPREGRLVKAATFSSSKWGWYAEAAPGTVVVRLSVGRHGEAAALQR
                     DDADLAAAAAAELGEIAGVSGPPVEIRVTRWGGALPQYAVGHRARVRRARAAIAEHPG
                     LAVCGAAYDGIGIPACIGSARAAVAQLDRAVPEPLGGLGTTR"
     gene            10467..11174
                     /gene="hemQ"
                     /locus_tag="VLC50_06415"
     CDS             10467..11174
                     /gene="hemQ"
                     /locus_tag="VLC50_06415"
                     /EC_number="1.3.98.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018844437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide-dependent heme synthase"
                     /protein_id="HSF27137.1"
                     /translation="MTDATSEPPATSAADDAVQYTMWSVFRATTPLGDADRTPLVAEV
                     SELFESLTSKGVVLRGVYDLAGFRADADVMIWWHAPTSDDLQEAYQRFRRTRLGRTLE
                     PVWSQMGVFRPMEFNRAHVPAFVEGVAPGRYASVYPYVRSYDWYLLPAQERAAMLAEH
                     GRLARDYPQVHGNTVASFALGDFEWMLAFEAEVLHDIVDMMRHLRSAEARRHTRLEIP
                     FYTGLRRPVDEIVATLP"
     gene            11171..11977
                     /locus_tag="VLC50_06420"
     CDS             11171..11977
                     /locus_tag="VLC50_06420"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HSF27138.1"
                     /translation="MRAKPGEASEGAPARIPAPRSHAPRHLRVRRPRSRVLAVLIDSW
                     TLLIASAVVFAGVLLGFLLPVDEAVAGWRLGVHYPSMHGLMEVLDHVGQRAVCLPLLA
                     AATALVAWRRRRWWPVLVAVSGVLAINVVVGAIKLTTDRPSPRTGTPELWQYGDLFPS
                     GHSANVVFVYGLMAWLLVRDTRHGRQWAGWLVGLVAAGALGMFVISIYRDTHWLTDLV
                     LGALIGALVLKWTIIADVRGDRDDEHDHLQRPRGSSAGSRRSDTELMHQR"
     gene            complement(11911..12342)
                     /gene="msrB"
                     /locus_tag="VLC50_06425"
     CDS             complement(11911..12342)
                     /gene="msrB"
                     /locus_tag="VLC50_06425"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009714399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HSF27139.1"
                     /translation="MDANKTYPVARSESEWRERLSPQEYAVLRKAGTERPFTGEYVDT
                     ETVGVYSCRACGAELFRSETKFHSHCGWPSFYEPTEADAVELIEDRSLGMVRVEARCA
                     ACGSHLGHVFEGEGYPTPTDQRWCINSVSLRLEPAEEPRGR"
     gene            complement(12360..13367)
                     /locus_tag="VLC50_06430"
     CDS             complement(12360..13367)
                     /locus_tag="VLC50_06430"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: protein motif:HMM:NF015047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HSF27140.1"
                     /translation="MLTTRLCDLLGITHPVVLGGMALGTNPDLVAAVSDAGGLGTLGG
                     CADRSPESIAELAAGIRELTDAPFGMNLLLFRVDETDERPTSPLQAVLRTRPAVLATA
                     WARADQDLSPVFADAHEAGCLVMHMVSTVAEADRAVAAGADVIVAQGSEGGGHVGLVG
                     TIALVPMVARAVAPVPVVAAGGLADGAGLAAALMLGAEGALFGTRFLATVEASVPDSY
                     KQAIVESDGHHTVLTELPDVAAATVWPGAYARVERNHFIDSWLGREGELRLRQREVGD
                     QVWAALRRGDMSGAPVYLGQSAGLVTAVEPAARVVERIVAEATERLHRRAFAADGPVP
                     D"
     gene            13473..14534
                     /gene="ligD"
                     /locus_tag="VLC50_06435"
     CDS             13473..14534
                     /gene="ligD"
                     /locus_tag="VLC50_06435"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012948080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HSF27141.1"
                     /translation="MAATPAIELDVRGRAVRVTNPEKLTFPAAGIRKIDVINYYVAVG
                     DGILRALDERPVTLERWQDGVHEGVKVATRADGRGADAFFQKRISKGAPDWVQTAHIT
                     FPSGRTADEVCPTELATVAWCAQQNTITFHPWPVRRDDVDHPDELRIDLDPQPGTDFA
                     DVVAVAAVAREVLAELGWRGFPKTSGNRGVHVYVRIQPRWTFTDVRHAAIAFGRELER
                     RMPGQVTTKWWKEERGQTVFVDYNQNARDRTIASAYSIRPRPHAPVSAPVEWDELPSV
                     DPRELTVLTMPARFAAIGDLHAGIDDVAHDLTPLLEWYERDERDHGLGDMPYPPDYPK
                     MPGEPMRVQPSRARTPAEG"
BASE COUNT         1866 a         5381 c         5619 g         2013 t
ORIGIN      
        1 caccgacgcc ggcaggcccg acagcagcgc ggcggtgcgg ccgatggtga gtccctggtc
       61 gccgatctgg gtggtggcgg cgatcgccac gtcgtcgacc cgctcggccg ggagggtggg
      121 gttgcgccgc ataagctcac ggatgcacgc gatgaccagg tcgtcggcgc gggtctcggc
      181 gtagatcccc ttgggcccgg ccttgccgaa cggggtccgg accccgtcga cgaacacgac
      241 ggcgcgacca gcgtgcccgt tgtggttggc cttgcggttg gcggcggtgt gggtgccggg
      301 catgagccgg tcagccgggt cggccatggt gctcctcacg acgtacgacg gagtgctccg
      361 gccgatgcta cccgctggta acaacggcgg ggttcacgcc gcggggaggt agagcacgag
      421 cagcgtcgcg ccctccggtc ccacgtcggt ggtggggtgc tccaggcctg gcgggacgtg
      481 gacgtaggag ccggcggtga gccggcgccc ggcgatggtg gcagcgccgg agatcaccca
      541 gacatggtgg tgggcggggt gcggctcgcc cgccgaccag ccgccctcgt ggtagtgcac
      601 cagggtctgc accatcgcgc cggagcgcca cagcaccttg taggccgcgc cggtgctccc
      661 ctgcagcggc gcccacggca gccggtcgat cgcgagcggg tcgagggcag tcagcgcctg
      721 gtcggcggcg gtcgtcgtca tggccctatg ccagcccttg cgggcgcatc cgggaagcga
      781 cgaaggtccc cggccctcag gcccggcggt ggaagggcgg tcgtcgtgct caggtcgggt
      841 gcgcgtcgag ccacccgagg gccgcctgga tgggatccgg ctgcttgtcg aagcgccgtc
      901 tcacgatctg gccgagggct tcggcggcgg tgtgacgcgt gtcgccgtcc actgtcgggt
      961 ctctcaggat ctcggccacg gcgggcaccg cctcgcgggc gcgcaggccg ccgagaccga
     1021 gcaggatcga gtcgcgctct tcgtctgcga ccgagcggcg caacgcgtcg agcagggcgg
     1081 caacgacctc ggggctcgac gtgccgatcc aggccagtgc gccggccgca tcctcacgag
     1141 agtccgcgct gtcagagtgc agcatcttca gcagaaacgg catggcctcc acaccgcgag
     1201 cgatgagtcc ccacgacgcc atgactcgct tgggaaagtc gtcctcgcgg atcgcttcca
     1261 gatggagtgc gtagtagtcg gaggccggcc accgcagctg cgtcagatcg ggcacaccgc
     1321 gcgcatcgat ggagactgtc tccagctgtg agcgacggcg aaacacgcga ccagtatggc
     1381 gagatcgcct gccgacgtca gcgcacgtcg agcggagcgc tcagctcggg ctctgcaccc
     1441 tcgacgatca cctgccactg accgggcgag gccagctcga cgacgagctc gccatcggcg
     1501 ccagcggcaa tctcgacctc gccggccacc gagccgtctg ggcgcttgat ctcgagcgtg
     1561 cgtgccccgt cggcgtcgtt gtcgagcttg aactcgatct tctcgccgac ggtggccgac
     1621 tcgccgtcga gcccggtgat gctggtgccg tcggtgccca gctcgagctc gcggtcgtag
     1681 gccgcctcac tgttcgatcc ggtgctggcc ccagcggcct ggtcgccggc caccgttacc
     1741 gtcgcccgga tgccgtcgcc ggtctggccg ggcttgcagg cgatctcgta ggtgccgccg
     1801 cccaggtcga cggtcatgtc acgcgacgtg ccggggccga tgttctccac ctcggagatg
     1861 accttggtga aggcgccgtc cttctcgccg tagacgtaga cctcggtcac ctttgacccc
     1921 gagttggtga cctcgaacgt cgtcgatccg gcggccagct cggacctcga gagcgtgcag
     1981 gaggtgtcgg tggcggtcac cgccacctct gagccggccg tgcccgaggc gtcggtggcc
     2041 gagtcgctgg agcagccggc gaggaccggc aggacgaggg cggcggtgac aaggccgagg
     2101 ccgcgcacga gctgccggga aaggggggtc ggacgcacgc gtgatctcct caggcgccgg
     2161 tcgcctgcgg gaccgatgcg gtggtggggg ttgtggggcg ttggcgggcg ggtcggaaga
     2221 agaggtagcc gaccggcacg aggtagccga cccagacgac gacctcgatc caggtcgagg
     2281 cgggcgagaa gttgaagatg cccttgagca gcgtgccgta ccagctgtcg ggcggcacct
     2341 gcgccgacac gtcgaaggcc aggctgtgca gcccgggcag gatccccgcc tcctgcaggt
     2401 cgtgcacgcc ataggcgagc acgccggccg ccaccacgat gagcgcggcg ccggtccagg
     2461 tgaagaactt ggccaggttg atccgcaccg cacccttgta gaacagccag ccgagcaccg
     2521 cggcggagag cagcccgagg gtggccccgg ccagcggggc ggcgcccgag ccggtcgcct
     2581 gcacggccgc ccagatgaac accgaggtct ccagcccctc gcggcccacg gcgatgaagg
     2641 cgagcaggac cagggcaccg gtgccgatcg cgaccgccga gtcgagacgc ccctgcagct
     2701 cggccttcat gaaccgcgcc gtacgacgca tccagaagac catccacgtg acgaagccga
     2761 cggcgacgat cgacagcgtg ccgccgaacg cctcctgggc ctcgaacgag aggtggcgcg
     2821 aggtgaacgt cagcagggcg ccgacgccca ggctcatcgc gatcgccacc cccacgccgg
     2881 cccagatcgg cgggatcttg cgctggtggc cggtcttgac caggtaggcg atgaggatgc
     2941 tcacgaccag tgaggcctcg agaccctcac gtagcccgat gaggtagttg gcgagcaccg
     3001 ggctcctccg cgacagacat cagatcggtt aggtgagcct aagcaaagac gatcagacgc
     3061 gcaagtcggc ggtcggtgcc gtcagggcgg tcacgagcag ctcggtgcac agcctcacct
     3121 gccaggcccg ggcaccgtgc tcggtgagga acgcggccac gctctccgcc tcgacgggtt
     3181 ggggcggtgc ccaggccagc cgccggacgg tgtcgggcgc cagcaggttc tcgaccggca
     3241 gggcgagctc gccggccagc tcggccaacc gggcccgggc atcggccagc cgggccgccg
     3301 ccgaggggtc gcggtcgcgc cagctgcggg ccggcggcgg tccctcggcc gggcgggtcg
     3361 gaggcggcag ggcgcgctcg ggcatccgcc gcgcagactc caccgcgccg gcccagtcac
     3421 gggcatagcg cttggcgcca cggccgacga acccgggcag ctccagcagg gcggcggtgt
     3481 cggcgggcag cgccatggcc gcctcgatga tcgccgagtc gggcagcagc cggctgggtg
     3541 acatgtcgcg ctcggcggcc agccggtcac ggatctccca cagggcgcgt acggcggcca
     3601 gctggcggcg cttgtggacc cggtgcagcc ccgaggtgcg tcgccacggg tcggctcgcg
     3661 gcgcgggcgg gggcgcggcc gcgatcgcgg cgaactcctc gcgggcccac ccgagcttgc
     3721 cctgccggtc gagctcggtc tcgagcgcgt cgcgcagctc gatgagcacc tcgacgtcta
     3781 acgccgcata gcgcagccac ggctccggta gcggccgggt cgaccagtcg gccgccgagt
     3841 ggcccttctc gagcaccagg ccgagcacgt gctcgaccag cgggccgagg ccgacccggg
     3901 ggaagccggc gaggcgggcc gcgagctcgg tgtcgaagat cgttctcggg tgcatgccga
     3961 gctcgcccag gcacggcagg tcctgcgaag cggcgtggaa gacccactcg tcgtcggcgg
     4021 cggcggccag cccggacagg tcggtgaacg ggacgggatc gacgagcacg gtgccggcgc
     4081 cccggcggcg cagctgcacg aggtaggcgc gctggccgta gcggtagccg ctggcacgct
     4141 cggcatcgat ggccaccggg ccgtggccgt gggccagccg gtcggccgcg tcggccagct
     4201 cggtcgggtc ggccaccacg cgggggaggc cctcgaccgg ctccaccagg ggtaccggcg
     4261 ccggatgagg cgcgggtacc gctggggcag tcacttctcc accgtacgac ctggccgtcg
     4321 ccggtccgtc agcggatcag cccaccccgc atcgccatgg ccaccatctc ggcccgatcg
     4381 ccggtgccga gcttgcgcgc gatccgggcg aggtggctct tgaccgtcag tgccgacagg
     4441 ccgagcgcct cgccgacgtc gcggttggtc cgcccgtcgc tgaccagccg gagcacctcg
     4501 atctcgcggg ccgacaggcc ggccagcccg tgtgcggccc gcttcgcccc gagcacctgc
     4561 aggggcgcca ccgggcgcgg agcgggtcgg ctcacgagca gggcccgtac gccgcaagag
     4621 agcgcggctc ggacgctaga tgggtcgtcg gagccggtga tcacgactcc gtgcggccag
     4681 cccgccgtgc gcagctcgtc gagcagggcc aaccccgacc cgtcgacgag ccccagctcc
     4741 acgacggcca ggtcgcgcgg gtcgccggcg cgggcgatgg tgcgggcctc ggccagggtg
     4801 gtcgcctcgg cgacgtcgtg ggcgcccagc gcgaacagcc gccgggtcac tgcctggcgg
     4861 gccggtgcgg cagcgagcac gaccagggcg gagaaccgtt cgggtcgccc ggcggtcgcg
     4921 gtcgccgtcg tggcacgccg cccggagcgc tcgaccactg cgaccacaac ccctcctgac
     4981 tgtcgtgacg acgagaagac ctgagacagg tctgcccgca tcatcggcgg atgggggaga
     5041 ggtctgaagc ggacccgggc ggtcggtcgg atcgaccggt caggccttgc gggtggcgcg
     5101 ccggttgggc aacgagacga tgcccggggg tagcggcgcc aggccggcgg cggtgcacaa
     5161 caggtcggcc caggccagca ggtgggcagc gacctggggc atcgggccgt cccaggccgg
     5221 ggtccacgag gcccgcagct cgagctcggc ctcgggcggg cgctcgctca tcgtgccgaa
     5281 ggactccgag ctgacccggg tcacggtgcc cccgagcacg agcgcctggg cggcgcgcgc
     5341 ctcgagcgcc tcggtcagcc aggcccaccc cacgctcgtg agcatcgggt cggccgccag
     5401 gtcggtctcg agctcggcgc gcacgaagct gaccacccgc caggtcccgg cccaggcgtc
     5461 gtggccggcc gggtcgtgca ggagcacgaa gcgcccggtc gccaggtcgt cgccgtcgac
     5521 ggtgacgtcg ccggtcagcg ccaccgcgtg cggcgccaac cgcgccggcg cgggcgcctc
     5581 ctcgatgtgc agctcggcgc gcaccgtgac ctcgcgcagc tggcgcagcg cgtgcgcgaa
     5641 cagctcggtc gggtcattga ccgagtcgga tgcgctccgc gcgcgtttcg cgccgcggct
     5701 gccgctcgcc acgatcgcag agtaagggcg agcgggctcc ctcaccgcgc ggcacgccga
     5761 agccggtcgc caccgctgcc ggcgcggctc gtagcctgcc cgccgtgagc ctggtcctgg
     5821 ccctcttctc gagcgtgctg tggggcggtg ccgacttctt gggcggcacg ctgacccgcc
     5881 gccgggcggt gctgcacgtg gtcggggcct cccaggcggt cggcctgctc accgtcgtgc
     5941 tcctggcgct gaccgccgga tcgttcggcg aacagggcga ctacctgcct tatgccgtcg
     6001 gggccggtct ggccggggcc gtcgggctgc tggccttcta cgccgccctg gcggcgggga
     6061 gcatgggcgt cgtctccccg atcgcggcgc tgggcgtggt cgtcccggtc ggggtcgggc
     6121 tggcccgggg agaggctccc tcacccgtcg tgctcgccgg gatcgccgtg gccgtccttg
     6181 gggtggtgct ggccagcggc ccggagctgc ggggacgtgc cggggcaaag ccgctgctgc
     6241 tcgcaggggt ggccgccctc ggcttcgggc tcgcgctgct gctcatcgcc gagggcgcgc
     6301 gcaccagccc gctcatgacg atggtcgggc tgcgagccgc ctcagtgggc gccgtggtca
     6361 cggtgctcct cgtggccgcc ctgcgccggc agctggcgcg tccggtcggt gccggcgagg
     6421 cagagccgcc gccggcgccg ccgtggcggc gagccgaggt ggcgctgctg gtggtcatcg
     6481 gggtcgccga cgtcatggcc aacgtggtgt tcgccctggc gacccgtggc gcctacgtga
     6541 gcgtcgtgag cgtgctcggc tcgctctacc cggtggtcac cgtgctcctg gcgatggggg
     6601 tgcacggcga acggctggcc cgggtgcaga ccatcgggat cgccaccgcg ctgggtgggg
     6661 tgcttctcat cgccggtggc tgaacgctcg tgcgacgatg cgcccgtgcc gagcccacgt
     6721 cctgacctcc gtgatgaccg gcccgacctg tccgggtcgg ccttcctgcg tgccgcgcgc
     6781 cgcgaaccgg tgccgtggac cccggtgtgg ttcatgcgtc aggccggccg cgcgctgccg
     6841 gagtaccgcc gggtgcgcga gggggtgccg atgctcgagg cctgcctgcg ccccgacctg
     6901 gtcgtcgaga tcaccatgca gccggtccgc cgctacggcg tggacgccgc ggtgctcttc
     6961 agcgacatcg tcctgccgct gcgggccgcg ggggtggacc tcgacctggt gcctggcacc
     7021 ggcccggtga tcgcggcccc attgcgcacc cgtcgtgacg tcaccgggtt gcccacgctc
     7081 gagcctgacc acgtcgcacc cgtggccgcg gccgtgcgtg gactggtcgg cgagctgggc
     7141 gcgaccccgc tgatcgggtt cgccggtgcg ccgttcacgc tggcctccta cctgatcgag
     7201 ggcgggccga gcaaggcgca cgtgcgcacc cgggccttca tgcatgccga accggcgctc
     7261 tggcacgagc tgctcggccg gctggcgacg ctgtcggcga cgttcctgcg ggtccaggtg
     7321 gaggccgggg cgtcggcggt gcagctgttc gactcgtggg tcggggccct gtcggcggcg
     7381 gactaccgcg agttcgtgct gccgcactcg cgggccgtgc tcgaggggat cgccgacctc
     7441 ggcgtgcccc gggtgcactt cggggtgggc accggtgagc tgctcgggct catgggccag
     7501 gccggtgccg acgtcgtggg cgtcgactgg cgggtgccgc tcgacgaggc ggtgcgcagg
     7561 atcgggccgg gccgcgcggt gcagggcaac ctcgacccgg cgctggcgct gtgcccgtgg
     7621 tcggtcctgc atccccacgt gctcgccgtg ctcgaggccg gccgagccgc cgagggccac
     7681 atcttcaacc tggggcacgg ggtgctgccg gagaccgacc ccgacacgct gacccggctg
     7741 gtcgagctcg tccacgagag caccgcccgc tagggcggtg ccggctccgg cgagggctcg
     7801 gcgtcggggc tcaggccggg gtgccgtcct cggcgggcgg gccggccggg cggcgtgcgc
     7861 gtcggcgcag cgccacccgc accgggatgg ccaccagcgc gccaaggacg aggaacggca
     7921 gcatcgcgcc gagggcggtc agcccgaccc cgacggccga cccgaacgcc gtccagccgg
     7981 cgcgcaggcc gccgaggaac ccgccggtgg ccgccgcgac cgtggtgccc ggcgggacca
     8041 gcgtgacgtt gaccgtggcc aggtcggtga ggttggccag ccgcttgcgc tgggcctcga
     8101 gcgactccag gtcggcctgc cggcgggtca gctcggactc gatctgcacg acctcgccga
     8161 gcgtgtcggc ccggtcgagc agcgcccgga cccgctcgac gctggcccgc gcggtggcca
     8221 cccgggcctc gacgtcgacc acctgcgcgg tgacgtcctc gctggtcagg tcgcgctcca
     8281 gcagggtgcc gaggtcggcc agcttgtcca tcgcctcgtc ggcacggtcg gtcggcaccc
     8341 gcagggtgag ctgggccgag tcgcgagcac ccttggggtg ttcgcccccg gcccggctgg
     8401 tcggcgtgtc ctcgccggca ccgccgatga tccgctcgga cgagaccacc ccacccagcg
     8461 tggtggccag gctccgggtc cgggccgcgg cgtcggagac gtccttgacc cgtacgacga
     8521 gcgagccggt caccacgagg tcgcggggga ccagggcgac cccggcggcg tcgggtgcac
     8581 cggccggcgg cttgccctcg gcggcggagg cgtcgcggtc ggcggtcgcc ccctgctcgg
     8641 tggcaccgcc ggccgtgccc gcgtcgagcc cgcccgagcc cgcatcgccg gcgctgaccg
     8701 gagccgccgc ctccgaagag cccgacgacg tgacagcccc gctgcaggcc gtcgcgaggg
     8761 ccaggccggc ggccaccagc agggtgagca gccccccgcg ccgtgcccgg ctggggcgtc
     8821 cggctggctt cgtcgtaccg gccatgggtc ctcccacgtt cgcttgctcg tgccggtcag
     8881 acggcacccg ggttggttcg gttccgcccg ggttctgcca gggtggggcg gtgcaggcga
     8941 gcgacgggca gggccggacg ggacgcggcg gggccgacgt ggtcgtggtg ggcgggggca
     9001 tcgccgggct ggccgccgcg tggtacctct cggagcgggc cggtcggcgg gtgacggtgc
     9061 tggaggccag cccccgggtc ggcggcaagc tgcacgcgtc gaacgtggcc ggcgccagcg
     9121 tcgacgaggg agccgagtcg ctgctcgtgc gccggcccga gggactcgcc ctggcccgcg
     9181 acctgggtct cggtgacgag ctggtcgccc cggcgacgct gcacgcgtcg gtcgtgcgcc
     9241 gcggtcggct gcacccgttg ccgcgcgggc aggtgatggg ggtgccggcc gacctcaccg
     9301 cgctggcccg gtccgggctg ctgaccgtgc ccgagctggc gcgcaccacc ttggaccacc
     9361 tgctgccgcg caccccgatg ggggaggacg tcgcgatcgg ccgtcaggtg cgggcccggc
     9421 tcggcgccgc ggtggtcgac cagctggtcg agcccctgct cggcggcgtc tacgccgggc
     9481 acgccgacga gctctccttc gacatgaccg tgccggccct gttcgccgcc gccacccgca
     9541 cccggtcgtt gctgcgcgct gccgccggcc tacgccgggc ctcgaccagc gcggtggagg
     9601 gcagcgacac gagtgcgcag gtgttcgcct cgcttcccgg tgggctggcc cggctggtcg
     9661 accggctggt cgagcggctg gccgagcgcg gcgtcagcct gctcacccgc accaccgccc
     9721 gggagctgcg acgcaccgag accggatggg cgctggtggt cggggccgcg agcgcgccgc
     9781 gcacgatgag cgccgacgcc gtcgtgctgg ccaccccgag cgccccggcc agccggctgc
     9841 tggcgggagt ggcgccggcc gcggccgccg agctggccgc gatcgactac gcgtcgatgg
     9901 cgatcatcac gctggcctac ccgcgggccg cgttcgcccg gatgccggcc gggtccgggt
     9961 tcctcgtccc gccccgggag ggtcggctgg tcaaggcggc caccttcagc tcgtcgaagt
    10021 ggggctggta cgccgaggcc gcgcccggca ccgtcgtcgt acggctgtct gtgggtcgtc
    10081 atggcgaggc cgcggcgctg cagcgggacg acgccgacct ggccgccgcc gccgccgccg
    10141 agctcggcga gatcgccggt gtgagcggcc cgccggtcga gatccgggtg acccgctggg
    10201 gcggggcgct gccgcagtac gccgtcgggc accgcgcgcg ggtgcgccgg gcccgggccg
    10261 cgatcgctga gcaccccggt ctggcggtgt gcggggcggc ctacgacgga atcggcatcc
    10321 ccgcgtgcat cggcagcgcc cgggcggcgg tcgcccagct cgaccgcgcc gtccccgaac
    10381 cgctgggcgg gctgggcacc acccggtgag ccgagtcacc ccgcccggcg cagcagcacg
    10441 gcgctgaagg ggggacaatg ccgtccatga ccgatgcaac cagcgagccc cccgccacca
    10501 gcgctgcgga cgacgccgtc cagtacacga tgtggtccgt cttccgggcc accaccccgc
    10561 tgggcgatgc cgaccggacg ccgctggtcg ccgaggtcag cgagctgttc gagtcgttga
    10621 ccagcaaggg tgtcgtgctg cgcggggtct atgacctcgc cgggttccgg gccgacgccg
    10681 acgtgatgat ctggtggcac gcgccgacca gcgacgacct gcaggaggcc taccagcgct
    10741 tccggcgcac ccgcctgggc cgcacgctcg agccggtctg gtcgcagatg ggcgtgttcc
    10801 ggccgatgga gttcaaccga gcccacgtcc cggcgttcgt cgagggcgtc gcacccggcc
    10861 gctacgccag cgtctacccc tacgtgcgct cctacgactg gtacctcctg ccggcccagg
    10921 agcgggccgc gatgctggcc gagcacggcc ggctggcccg cgactacccg caggtccacg
    10981 gcaacaccgt cgcgtcgttc gccctcggcg acttcgagtg gatgctggcc ttcgaggccg
    11041 aggtgctgca cgacatcgtc gacatgatgc gccacctgcg ctcggccgag gcccgtcggc
    11101 acacccgcct cgagatcccg ttctacacgg gcttgcgccg gccggtcgac gagatcgtcg
    11161 ccaccttgcc gtgagggcca agccgggcga ggcgagcgag ggggcgcccg cgcgcatccc
    11221 cgcaccgcgc agccatgccc ctcgacacct gcgggtgcgc cgacctcggt cgcgggttct
    11281 cgcggtgctc atcgactcgt ggaccctgct gatcgcctcg gcggtggtgt tcgccggcgt
    11341 gctgctgggc ttcctgctgc ccgtcgacga ggccgtcgcg ggctggcggc tgggcgtgca
    11401 ctacccgtcg atgcacgggc tgatggaggt gctcgaccac gtcgggcaac gcgcggtctg
    11461 cctgccgctg ctcgccgccg cgaccgcgct cgtcgcctgg cgtcgccggc gctggtggcc
    11521 ggtgctggtc gccgtcagcg gcgtgctggc gatcaacgtc gtggtcggtg cgatcaagct
    11581 gaccaccgac cgacccagcc cgcgcaccgg gacccccgag ctgtggcagt acggcgacct
    11641 gttcccgtcc gggcactcgg ccaacgtggt gttcgtctac ggcctgatgg cctggctgct
    11701 cgtgcgcgac acccggcacg ggcgccagtg ggcgggctgg ctggtggggc tggtcgcggc
    11761 cggtgcgctc ggcatgtttg tcatctcgat ctaccgcgac acgcactggc tgaccgacct
    11821 ggtgctcggc gcgctcatcg gcgcgctcgt cctcaagtgg acgatcatcg ccgacgtcag
    11881 gggtgaccgg gacgacgagc acgaccacct tcagcgcccg cgcggctcct cggccggctc
    11941 gaggcgcagc gacaccgagt tgatgcacca gcgctgatcg gtgggcgtcg ggtagccctc
    12001 gccctcgaag acgtggccca ggtgcgagcc gcacgccgcg caccgcgcct cgacccgcac
    12061 catgcccagg ctgcggtcct cgatcagctc gaccgcgtcg gcctcggtcg gctcatagaa
    12121 cgacggccag ccgcagtgcg agtggaactt ggtctcgctg cggaacagct cggccccgca
    12181 ggcccggcag gagtagacgc cgaccgtctc ggtgtcgacg tactcgccgg tgaacggccg
    12241 ctcggtgccg gccttgcgca gcacggcgta ctcctgcggg ctcagtcgct cccgccactc
    12301 ggactcgctg cgtgccacgg ggtaggtctt gttcgcgtcc acctctccac cgtacgacgt
    12361 cagtcgggca ccggcccgtc cgcagcgaag gcccgccggt ggaggcgctc ggtggcctcg
    12421 gccacgatcc gttcgacgac ccgggcagcg ggctctaccg cggtgaccag accagcgctc
    12481 tgcccgaggt agaccggtgc gccgctcatg tcgccgcgac gcagcgcagc ccacacctgg
    12541 tcgccgacct cccgctggcg cagacgcagc tcgccctcgc ggccgagcca ggagtcgatg
    12601 aagtggttgc gctccacgcg cgcgtaggca ccgggccaga ccgttgcggc ggcgacgtcg
    12661 ggcagctcgg tgagcacggt gtggtgcccg tcgctctcga cgatcgcctg cttgtagctg
    12721 tcggggaccg acgcctcgac ggtggcgagg aaccgggtcc cgaacagcgc gccctccgct
    12781 ccgagcatca gggcggcggc cagcccggcg ccatcggcca ggccgccggc ggcgaccacc
    12841 ggcaccggcg cgaccgcccg ggccaccatc ggcaccagtg cgatggtgcc gacgagcccg
    12901 acgtgcccgc cgccctcgct gccctgggcg acgatgacgt cggcgcccgc ggcgactgcc
    12961 cggtcggcct cggccaccgt cgagaccatg tgcatcacca ggcatcccgc ctcatgggcg
    13021 tcggcgaaca caggggacag atcttggtcg gcgcgggccc aggcggtggc gagcaccgcc
    13081 ggccgggtcc gcagcaccgc ctgcaacggg ctggtaggtc gctcgtcggt ctcgtcgacc
    13141 cggaacagca gcaggttcat gccgaacggc gcgtcggtga gctcgcggat gccggccgcc
    13201 agctcggcga tgctctcggg ggagcggtcg gcgcacccgc ccagggtgcc cagcccgccg
    13261 gcgtcgctga ccgcggccac caggtctggg ttggtgccca acgccatccc gccgagcacg
    13321 acggggtggg tgatgccgag caggtcgcac agtcgcgtgg tgagcatgtc gtcctcccga
    13381 ggtcgtgccc cgctgctctg tcgaccgtgg cacccgcgcg ggtgcggcgg tagtggggcg
    13441 gacggtctcg ccgagccggt agcgtgccga gcgtggctgc gaccccagcg atcgagcttg
    13501 acgtgcgcgg gcgcgccgta cgcgtgacca acccggagaa gctgacgttc ccggccgccg
    13561 gcatccgcaa gatcgacgtg atcaactact acgtcgcggt cggcgacggc atcctgcgcg
    13621 cgctcgacga gcgccccgtg acgctggagc gctggcagga cggcgtgcac gagggcgtca
    13681 aggtggccac ccgggccgat ggccgcggtg ccgacgcttt tttccagaag cggatcagca
    13741 agggcgcgcc cgactgggtg cagaccgcgc acatcacgtt ccccagcggg cgcaccgccg
    13801 acgaggtctg cccgaccgag ctggcgacgg tggcctggtg cgcgcagcag aacacgatca
    13861 cgttccatcc ctggccggtg cgccgcgacg acgtggacca ccccgacgag ctgcgcatcg
    13921 acctggaccc gcagccgggc accgacttcg ccgacgtggt cgcggtggcc gcggtggcgc
    13981 gcgaggtgct tgccgagctg ggctggaggg ggtttcccaa gacgtcgggc aaccgcgggg
    14041 tgcacgtcta cgtgcggatc cagccgcgct ggacgttcac cgacgtacgc catgccgcga
    14101 tcgcgttcgg acgggagctg gagcgacgga tgcccgggca ggtgaccacc aagtggtgga
    14161 aggaggagcg cgggcagacg gtctttgtgg actacaacca gaacgcccgc gaccgcacca
    14221 tcgcctctgc ctacagcatc cgcccgcgcc cgcacgcgcc ggtctcggcg ccggtcgagt
    14281 gggacgagct gccctcggtc gacccgcgcg agctcaccgt gctgaccatg ccggcgcgct
    14341 tcgccgcgat cggtgacctg cacgccggga tcgatgacgt cgcccacgac ctgacgccgc
    14401 tgctggagtg gtacgagcgc gacgagcgcg accacgggct gggcgacatg ccgtatccgc
    14461 cggactaccc gaagatgccc ggcgagccga tgcgggtgca gcccagccgg gcccgcaccc
    14521 ccgccgaggg ctgacccgcc gtcccctggg cggcgtccct ggggaaagcc ccggtgcccg
    14581 gggcgcggcg cccctgtgga agctccggtg cccggggcgg cgatcaagca acttctgcac
    14641 ggatcgggac cattgcgggc ccaccgggga ccttggtcac acgctttggc atgcccacat
    14701 gcgttgtcat gaggtgtcgc aacgcatgaa aggggagaac cgtggaaaga cgagcacttg
    14761 accggttgct gtcagcggcc ggcgcagtga tggccctggt gttgctggtc ggtggagtgc
    14821 tggccctggt gggccacaac ttcgccacca accaggtgcg cgaccagctg gtgcaggag
//
LOCUS       DATIBB010000167         7158 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_629215, whole genome shotgun sequence.
ACCESSION   DATIBB010000167 DATIBB010000000
VERSION     DATIBB010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 7158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7158
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_629215"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1119
                     /locus_tag="VLC50_06440"
     CDS             <1..1119
                     /locus_tag="VLC50_06440"
                     /EC_number="2.7.7.65"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HSF27142.1"
                     /translation="DLMLVEVAARLSAAVPADQMVARLGGDEFAVLVQERDLDQVAAA
                     AARVVRAFDPPMLVQGREHHVSMSVGVALREAAVHDPLELLRAADVALYDAKDRGRNR
                     FAVYTADMADRVEETMRLEQRLGTAVHDGEITVVFQPVLALATQRVVGVEALARWSVD
                     GQAVPPDVFIPLAEGRGLIGELGEHVLDLALGAFAGWRASGRDIDHVAVNISPMQLED
                     HRVLLTVRALLDRHRLPPSALVLEVTESTVMTDSGPALQVLDELAGMGVAISLDDFGT
                     GFSSLARLRALPVSEVKIDRSFVSDVGADEALLRAMVLMARGLGLRTVAEGVETEQQL
                     DALESMGCSAVQGYLLSRPLPEAEVYRRLAEPAHLVHR"
     gene            1218..1805
                     /locus_tag="VLC50_06445"
     CDS             1218..1805
                     /locus_tag="VLC50_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018382927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03086 family metal-binding protein"
                     /protein_id="HSF27143.1"
                     /translation="MTSQQTDTRELFRQAVDGFGRRVHAVAPDQWHDPTPCTEWDVRA
                     LVSHLVDEALWATPLLDGATLQDASTLVPADPLAGDQDPVSAWERAADALREELAERM
                     DLDAIVHLSYGDVPAREYVEELTFDFAVHSWDLARAVGADESIDPPILEAAWSYLGRH
                     GEGLAASGRFGPVVDVPDDADTQTRVLAATGRAAR"
     gene            complement(1802..2710)
                     /locus_tag="VLC50_06450"
     CDS             complement(1802..2710)
                     /locus_tag="VLC50_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011984914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HSF27144.1"
                     /translation="MTRRGWVLFLGLGVIWGVPYLLIKVAVAEVSPVTLVLARTAVGA
                     LVLIPLAARRGLLGPVLARWKPLLAFTLAEICVPWWFLSEAERHLSSSLAGLLVAAVP
                     LVGAVLVQLTGQERLDRRRAVGLVAGLGGVAALVGFDVHTDDLAAVAAVGIVVIGYAI
                     GPIILARYLSDLPALGVMAAALVLASILIGPFGVAAMPTSWPSQQALLALLVLGVVCT
                     ATAFLMFHGLIAEVGAARATVVAYVNPAVAVVLGVVVLGERFTWATALGFGLILIGSV
                     LATAAGRAKAAPAPEPSAVTANQPVG"
     gene            3041..3298
                     /locus_tag="VLC50_06455"
     CDS             3041..3298
                     /locus_tag="VLC50_06455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF27145.1"
                     /translation="MTPAADDPAGPVGRTGFRLGVLALAVLGTLMMLATVLPTGPSRH
                     EPAPAPSPAVTQQTGRGVLDGHSVTEEPAPGQPVFQGPFGP"
     gene            3326..3517
                     /locus_tag="VLC50_06460"
     CDS             3326..3517
                     /locus_tag="VLC50_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005440210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5302 domain-containing protein"
                     /protein_id="HSF27146.1"
                     /translation="MSSESTDPDAPNGPDELREKFREALARKQGRGGEAGSSGRTGPA
                     RAPHTAPAKPQRSFRRKSG"
     gene            complement(3525..4532)
                     /locus_tag="VLC50_06465"
     CDS             complement(3525..4532)
                     /locus_tag="VLC50_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015355326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HSF27147.1"
                     /translation="MPADTSIDIKIHNTVDPHTDSEADLDAPAPSADLVRVYLDEIGR
                     TPLLTAEEEVDLSKRIEAGLYAEHLLAEEADGSKRRLSATRRRELRAVAADGQTAKAA
                     MISANLRLVVSIARKFGQRGVPMLDVVQEGNLGLIRAVEKFDYRKGYKFSTYATWWIR
                     QSIGRGISEQARTIRLPVHVTEAISKIARAQRQLVQELGREVTDEDIAASTGLDVERV
                     VELRAVTRDALSLDTPVGEGETSVGDLVADTGVASIDEVIDHAVLVEELHRTLDELPE
                     RERRIISLRFGLEDGRQHTLDEVGRVVGLTRERVRQLERDTLRTLSQPEHRERLLDWA
                     S"
     gene            complement(4654..6393)
                     /locus_tag="VLC50_06470"
     CDS             complement(4654..6393)
                     /locus_tag="VLC50_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HSF27148.1"
                     /translation="MAQAVQTQPHTSALPDSPLLRRIRRSIIGDDQVLPGPYGPRRVT
                     YADYTASGRALAFLEAFIRDEVLPRYANTHTESSGTGLQTTRLREDAREIIRDAVGGD
                     ESTAVIFTGSGSTGAIDKLVGILGLRVPSALEDRWHVTDAIPAHERPVVFIGPFEHHS
                     NDLPWRESIADVVTIPEDADGHVDLARLESELVRYADRPLRIGSFSAASNVTGIVTDT
                     ARVTRLLHEHGALSFWDFAAAGPYVEIEMNPAGDPAAAKDAIFLSPHKLVGGPGTPGV
                     LVVRRDLLTNRVPVVVGGGTVAYVNPTEHVYLDDPEHREEGGTPAIIGSIRAGLVFQL
                     KAAVGIDVIRAHEEHFLARALAAWTANPAIEVLGNLTAERLSILSFVVRRPGGRYLHH
                     NFVVAVLNDLFGIQSRGGCSCAGPYGHRLLGIDLDRSHEFENQITRGCEGIKPGWVRV
                     NFNYFISEAVFRYVVEAVDLVAREGWRLLPDYRFEPATGLWRHRSGPVEPPLRLRDVH
                     YTDDGALAYPAHRSRAPEAALRDYLDEARELLAARTDPDLGTCPRVARPALVDDEFER
                     LRWFELPAASLSP"
     gene            complement(6412..>7158)
                     /locus_tag="VLC50_06475"
     CDS             complement(6412..>7158)
                     /locus_tag="VLC50_06475"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HSF27149.1"
                     /translation="IAITHAHGDHCLGLPGVLARRALDGAPEPVVLHYPAPAQADIDA
                     LRRAGRPAQPAPVTEAPVDADGVIATGPTWRLLAAALDHRVPAVGYRLEERPGVRMRP
                     ERLAAYGVSGPDVGRLRREGQLTRPDGTTVRLDAVSEPRPGQVFAFIMDTRWCAGALR
                     LAQQADLLVVEATFLDRDRELAAGYGHLTARQAGVLAAEAGARRLVLTHFSRRYGDDT
                     TPFLAEASAAFDGQVVAAADLDVIPVPPRR"
BASE COUNT          998 a         2512 c         2700 g          948 t
ORIGIN      
        1 gacctgatgc tcgtcgaggt ggccgcgcgg ctgtccgccg ctgtcccggc cgaccagatg
       61 gtcgcccgcc tcggcggcga cgagttcgcc gtactcgtcc aggagcgcga cctcgaccag
      121 gtcgcggccg cggccgcccg ggtcgtccgc gccttcgacc cgcccatgct cgtgcagggg
      181 cgtgagcacc acgtgagcat gtcggttgga gtggcgctgc gagaggcggc ggtgcacgac
      241 cccttggagc tgttgcgggc cgccgacgtg gcgctctacg acgccaagga ccgtgggcgc
      301 aaccggttcg ccgtctacac cgcggacatg gccgaccggg tcgaggagac gatgcgcctg
      361 gagcagcgcc tcggcacagc agtgcacgac ggcgagatca ccgtggtgtt ccaacccgtc
      421 ctggccctgg ccacccaacg ggtcgtgggg gtggaggcgc tggcccgatg gtcggtcgac
      481 gggcaggccg tgccgccgga cgtgttcatt ccgctggccg aggggcgcgg tctgatcggt
      541 gagctgggcg agcacgtgct cgatctcgcg ctgggcgctt ttgcaggttg gcgggcgagc
      601 gggcgagaca tcgaccatgt ggcggtcaac atctcgccga tgcagctgga ggaccaccgg
      661 gtgctcctca ccgtgcgcgc gttgctcgac cggcaccggc tgccgccctc cgcgctggtt
      721 ctcgaggtca ccgagagcac ggtgatgacc gactccggcc cagctctgca ggtgctcgac
      781 gagctcgccg gcatgggggt ggcgatctcg ctcgacgact tcgggaccgg cttctcctcg
      841 ctcgcccggc tgcgggcctt gccggtctcc gaggtcaaga tcgaccggtc gttcgtctcg
      901 gacgtcgggg cagacgaggc actgctgcgg gccatggtcc tcatggcccg tgggctcggc
      961 ctacgcaccg tggcggaggg ggtcgagacc gagcagcagc tggatgcgct cgagtcgatg
     1021 ggctgctcgg ccgtgcaggg ctacctgctc tcgcgcccgc tgccggaggc tgaggtctac
     1081 cggcgcctgg ccgaacccgc tcacctcgtg caccgctgac tcgacccgcc gttgggatgt
     1141 tcggccggtt gccgcagcgt ctgccggggc gcagactccg agaagcacgg gccgccgacg
     1201 ccgaggatgg ggtggcgatg accagccagc agaccgacac gcgtgagctg ttccggcagg
     1261 cggtcgacgg gtttgggcgg cgcgtgcatg ccgtggcgcc cgaccagtgg cacgacccga
     1321 caccgtgcac cgagtgggac gtccgagcgc tcgtgagcca cctcgtcgac gaggcgctct
     1381 gggcgacgcc gctgctcgac ggggcgacac tgcaggacgc ctccaccctg gtgccggccg
     1441 atccgctcgc cggcgaccag gacccggtgt cggcgtggga gcgcgctgcc gacgccttgc
     1501 gtgaggagct cgccgagcgc atggaccttg acgcgatcgt gcacctgtcc tacggcgacg
     1561 tcccggcccg cgagtatgtc gaggagctga cgttcgactt cgcggtgcac tcgtgggacc
     1621 tggcccgagc ggtcggcgcc gacgagtcga tcgacccgcc gatcctcgag gcggcctgga
     1681 gctacctcgg acggcacggt gagggcctgg cggcctcggg gcgcttcggc ccggtcgtgg
     1741 acgtgcccga cgacgcagac acccagaccc gggtgctcgc cgccaccggc cgggcggcgc
     1801 gctagcccac cggctggttg gccgtcaccg cagagggttc tggcgcgggc gccgccttgg
     1861 cccggccggc ggcggtggcc aggaccgagc cgatcaggat caggccgaac ccgagtgccg
     1921 tggcccaggt gaaccgctcg ccgaggacca cgacgccgag aacgacggcc acggcggggt
     1981 tgacgtaggc cacgacggtg gcccgggccg cgcctacctc ggcgatcaac ccgtggaaca
     2041 tcaggaatgc cgtggcggtg cagaccacgc cgaggacgag cagcgcgagc agcgcctgct
     2101 gcgagggcca tgacgtgggc atcgcggcca cgccgaacgg accgatgagg atcgaggcga
     2161 gcaccagtgc cgccgccatg acgcccaggg cgggcaggtc ggagagatag cgagccagaa
     2221 tgatcggccc gatcgcatag ccgatcacga cgatgccgac ggccgcgacc gcggcgaggt
     2281 cgtcggtgtg cacgtcgaac ccgaccagcg ccgcgactcc gccgagcccg gccaccagcc
     2341 cgaccgcacg gcgccggtcg agccgttcct gaccggtgag ctgcacgagc acggccccga
     2401 ccagcggtac ggcggcgacg agcaggccgg ccaacgagct cgacaggtgc cgctcggcct
     2461 cggagaggaa ccaccagggc acgcagatct cggccagcgt gaacgccagc agcggcttcc
     2521 agcgggcgag caccggcccg agcagcccgc ggcgcgccgc cagcgggatg aggaccagcg
     2581 cgccgaccgc ggtgcgcgcg agcacgaggg tgaccgggga cacctctgcg accgcgacct
     2641 tgatcagcag gtagggcacg ccccagatga cgccaaggcc gaggaacagc acccagccgc
     2701 gccgcgtcat ccgctactcc tatggtcggt cgccgtcagt accaagtatc aatgatgctc
     2761 ctgacattcc cgggcaggcg gcggggttcg gctcgggccc gaggatggga ccaacgtcac
     2821 tcccgccccc tccgcctgcg tgcctaccgt gcaaagtgca cctcgagcac aagggggcgc
     2881 catggagcct cagggcgcac tcgacgatgg ctccgctccg ctgtcccggc gcgagattcg
     2941 ccagttgcgc cgcgagttcg ccaagctcga tcggctcgga ggtcgccgcc cgcccaatcg
     3001 ggagtcggtg ccagcccctc gccggccgca ggacgacgcg atgacgccgg cggcggacga
     3061 cccggcaggt ccggtaggcc ggaccgggtt tcgcctgggc gtgctggcgc tcgcggtgct
     3121 gggcacgctg atgatgctgg cgaccgtgct gcccaccggg ccgagccggc acgagcccgc
     3181 gccggcaccg agccccgcgg tgacccagca gaccggccgc ggggtgctgg acgggcactc
     3241 ggtcaccgag gagccggccc ccgggcagcc ggtcttccag ggtcccttcg gaccctgacg
     3301 ccgcgacagg tgggacgatg ccgccatgtc tagcgaatcg accgacccgg acgcccccaa
     3361 cggccccgac gagctgcgcg agaagttccg cgaggccctc gcgcgcaagc agggccgcgg
     3421 cggcgaggcg ggctcgtcag ggcgcaccgg cccggcgcgg gcgccgcaca cggcaccggc
     3481 caagccgcag cggtccttcc gccgcaagag cggctgagca cgcctcagct ggcccagtcg
     3541 agcagccgct cgcggtgctc cggctggctc agcgtgcgca gcgtgtcgcg ctcgagctgg
     3601 cggacccgct cacgggtgag cccgaccacc cgcccgacct cgtcgagggt gtgctgacgg
     3661 ccgtcctcga ggccgaagcg gagcgagatg atgcgccgtt cacgctcggg cagctcgtcg
     3721 agggtgcggt gcagctcctc gaccagcacc gcgtggtcga tcacctcgtc gatcgaggcg
     3781 acgcccgtgt cggcgaccag gtcacccacc gaggtctcgc cctcaccgac cggcgtgtcc
     3841 aggctgagcg cgtcgcgggt caccgcccgc agctcgacga cccgctcgac gtcgagcccg
     3901 gtggacgccg cgatgtcctc gtcggtgacc tcccggccga gctcctgcac gagctgccgc
     3961 tgcgcgcggg cgatcttgct gatcgcctcg gtgacgtgca cgggcagccg gatcgtgcgg
     4021 gcctgctcgg agatccctcg gccgatggac tggcggatcc accaggtggc gtaggtggag
     4081 aacttgtagc ccttgcggta gtcgaacttc tccaccgcgc ggatcagccc caggttgcct
     4141 tcctgcacga cgtcgagcat cggcaccccg cgctggccga acttgcgggc gatggagacc
     4201 accagccgca ggttggccga gatcatcgct gccttggcgg tctggccgtc ggcggccacg
     4261 gcgcgcagct cgcgccgacg cgtcgccgac aggcggcgct tggacccgtc tgcctcctcg
     4321 gccagcaggt gctcggcata gaggccggcc tcgatccgct tggacaggtc gacctcctcc
     4381 tcggcggtga gcagcggggt gcgcccgatc tcgtcgaggt agacgcgcac gaggtcggcg
     4441 gagggcgccg gcgcgtcgag gtcggcctcg gagtcggtgt gggggtcgac ggtgttgtgg
     4501 atcttgatgt cgatgctggt gtcagcgggc atgcgtgacc tcccctcacg ggtgggaagc
     4561 agggctactg actgctacaa cagcgaacgc gtgtgcaaac gttcccggca tgggggtgag
     4621 caaacccgtc gtacgtcgcg ggcagggctg gcgtcagggc gacagactgg cggccggcag
     4681 ctcgaaccag cgcagccgtt cgaactcgtc gtcgaccagg gccgggcgtg ctacccgcgg
     4741 gcaggtgccg aggtcggggt cggtgcgcgc ggccagcagc tcgcgcgctt cgtcgaggta
     4801 gtcgcgcagc gccgcctcgg gggcgcgaga ccgatgggcc gggtaggcca gcgccccgtc
     4861 gtcggtgtag tggacgtcac gcagccgcag cggcggctcg accggcccgg agcggtggcg
     4921 ccacagcccg gtggccggct cgaagcggta gtcgggcagc agccgccagc cctcgcgcgc
     4981 caccaggtcg accgcctcca ccacgtagcg gaagaccgcc tcggagatga agtagttgaa
     5041 gttgacccgc acccagcccg gcttgatgcc ctcgcaaccg cgggtgatct ggttctcgaa
     5101 ctcgtgcgag cggtcgaggt cgatgcccag cagccggtgc ccgtaggggc cggcgcagga
     5161 gcagccgccg cgtgactgga tgccgaacag gtcgttgagc accgcgacga cgaagttgtg
     5221 gtgcaggtag cgcccaccgg gtcgccgtac gacgaaggac aggatcgaca gtcgctcggc
     5281 ggtcaggttg cccagcacct cgatcgccgg gttggccgtc caggccgcca gcgcccgcgc
     5341 caggaagtgc tcctcgtggg cccggatgac gtcgatgccg accgccgcct tgagctggaa
     5401 gaccagcccg gcccggatcg acccgatgat cgccggggtg ccgccctcct cgcggtgctc
     5461 cgggtcgtcg aggtagacgt gctcggtggg gttgacgtag gcgaccgtgc cgccgccgac
     5521 caccaccggc acccggttgg tcagcaggtc acgacgtacg acgagcacgc ccggggtgcc
     5581 gggcccgccg accagcttgt gcgggctgag gaagatcgcg tccttggccg ccgcggggtc
     5641 gccggccggg ttcatctcga tctcgacgta gggcccggcg gcggcgaagt cccagaagga
     5701 cagcgcgccg tgctcgtgga ggagccgggt gacccgggcg gtgtcggtga cgatgccggt
     5761 cacattgctg gcggcggaga acgacccgat ccgcagcggc cggtcggcgt agcggaccaa
     5821 ctcggactcc agccgcgcca ggtcgacgtg cccgtcggcg tcctcgggga tcgtgacgac
     5881 gtcggcgatc gactcccgcc agggcaggtc gttggagtgg tgctcgaacg ggccgatgaa
     5941 gaccaccggg cgctcgtggg ccgggatggc gtcggtgacg tgccagcggt cctccagcgc
     6001 cgagggcacc cgcaggccga ggatcccgac cagcttgtcg atcgccccgg tcgagccgga
     6061 cccggtgaag atcaccgcgg tgctctcgtc accacccacc gcgtcgcgga tgatctcgcg
     6121 cgcgtcctcg cgcagccggg tcgtctgcag gccggtgccc gaggactcgg tgtgggtgtt
     6181 ggcgtagcgg ggcagcacct cgtcacggat gaatgcctcg aggaaggcca gggctcgccc
     6241 gctcgcggtg tagtcggcgt aggtgacccg gcgcgggccg taggggccgg gcaagacctg
     6301 gtcatcgccg atgatgctgc gccggatccg ccgcagcagc ggcgagtcgg gcagcgccga
     6361 ggtgtgcggc tgggtctgga ctgcctgggc catgccccga tggtggcacg cctagcgtcg
     6421 cggcggcacc gggatcacgt cgaggtcggc ggcggcgacc acctggccgt cgaacgcggc
     6481 ggacgcctcg gcgaggaagg gcgtcgtgtc gtcgccgtac cggcgggaga agtgggtgag
     6541 caccaggcgc cgggcgcccg cctcggcggc caggacgccg gcctggcggg cggtgaggtg
     6601 tccgtagccc gcggcgagct cgcggtcgcg gtcgaggaag gtcgcctcca ccacgagcag
     6661 gtcggcctgc tgagccagcc gcagcgcgcc ggcgcaccag cgggtgtcca tgatgaacgc
     6721 gaagacctgg ccgggtcgtg gctcgctgac cgcgtcgagg cgcaccgtgg tcccgtcggg
     6781 ccgggtgagc tgaccctcgc ggcgcagccg gccgacgtcg gggccgctga cgccgtacgc
     6841 cgccaaccgc tccgggcgca tccgcacccc ggggcgctcc tcgagccggt agcccaccgc
     6901 cggcacccga tggtcgaggg ctgcggcgag cagtcgccag gtcgggcccg tcgcgatgac
     6961 cccgtcggcg tcgacaggtg cctcggtgac cggagccggc tgggccgggc ggccggcccg
     7021 gcgcagcgcg tcgatgtcgg cctgggcggg cgccgggtag tgcagcacga ccggctcggg
     7081 cgcgccgtcg agcgcgcgac gggccagcac gccgggcagg cccaggcagt ggtcgccgtg
     7141 ggcgtgggtg atggcgat
//
LOCUS       DATIBB010000168         5031 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_497386, whole genome shotgun sequence.
ACCESSION   DATIBB010000168 DATIBB010000000
VERSION     DATIBB010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 5031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5031
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_497386"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..387
                     /locus_tag="VLC50_06480"
     CDS             <1..387
                     /locus_tag="VLC50_06480"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HSF27150.1"
                     /translation="RGIAARFAVADALALAQLGETFDTAIDSGTFHLFAGPARSAYVQ
                     SLSTVLKLGGRLYLLAARDGGSGGWGPPGLSRAELVAAFAGGWRLEALESSRYDVTAA
                     APVDHVDAWLATLARTPAREGDVAAD"
     gene            complement(580..906)
                     /locus_tag="VLC50_06485"
     CDS             complement(580..906)
                     /locus_tag="VLC50_06485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF27151.1"
                     /translation="MPTLDLVDETTVRLTMRFAETPPPRWLAAFTAELAAGCCSEPVV
                     TPGCGRHEGCALAVDFAIEQVGRALTELGHAFRRANQQQRLDDQRLADLRGWLDRWWQ
                     QLIVTP"
     gene            complement(949..1545)
                     /locus_tag="VLC50_06490"
     CDS             complement(949..1545)
                     /locus_tag="VLC50_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019432402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase N-terminal
                     domain-containing protein"
                     /protein_id="HSF27152.1"
                     /translation="MTTQLDGTAWASALDEAIVAATEAMAAADAVDWSRPAGDLGWDC
                     RATVLHIASDFIGYATQLTAPRAHGYAALDLVLEGTPNPAELRALVRATGGLLSSVAR
                     TADAGTRSWHPYGVAGPCDFAAMGIVELLVHTEDLSRGLGFAWAPAPDLSARVLAHLF
                     PYAPRGHEPWPTLLWATGRAALGERPRLPDWRWENTGG"
     gene            complement(1542..1925)
                     /locus_tag="VLC50_06495"
     CDS             complement(1542..1925)
                     /locus_tag="VLC50_06495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF27153.1"
                     /translation="MPATYRYTCCQASDIERPWHPGDLFVLHWISEALPMTEARPPTP
                     VMLTAELAGPFKTVDRLKTATTAAPFAQATSARTTTHGGKALTSTILIPADAAPGLYR
                     LNFAIDSAGEILSGSSVIEIVRVKP"
     gene            complement(2048..2698)
                     /locus_tag="VLC50_06500"
     CDS             complement(2048..2698)
                     /locus_tag="VLC50_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019854661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HSF27154.1"
                     /translation="MARPGVPLLSRERIRDTALALIDAHGLDALSMRGLARELGVQAA
                     SLYGHFPTKDAVLDAVANLLTRDVDTSGFTRSWQEGLRVWGRSYHASLRAHPRAAPVV
                     AAGTGRREDFLAMADAVHGGLLRHGWPPRQATMIAASVKYLVIGAATTPFGSGFADDT
                     EVYLARYPHLVKAHLIRQDAERIDAESFELALTSLIAGLEPRHAELCAPDGDRASS"
     gene            2748..3920
                     /locus_tag="VLC50_06505"
     CDS             2748..3920
                     /locus_tag="VLC50_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005261379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HSF27155.1"
                     /translation="MAAPVSPLGLFGTDELVGEDDRAIAETVRRFVDDRIRPHVAQWY
                     DAAAIPARELAKELGSLGVLGMHLHGYGCAGTSATAYGLTCLELEAGDSGLRSLVSVQ
                     GSLAMYAIYAHGSEEQKQEWLPRMAAGEALGCFGLTEPDAGSDPAKMRTRARRDGDDW
                     VLSGTKMWITNGSVADVAIVWAQTDERIRGFVVPTNTPGFSAPEITRKLSLRASVTSE
                     LVLDQVRLPASAVLPGAVGLSGPLSCLTEARFGIVFGALGAARDCLETAISYVRDREV
                     FGKSLAGFQITQTKLADMALELGKGMLLALHLGRLKDTGTLRPEQVSLGKLNSTREAI
                     AIARECRALLGAAGITLEHPVMRHANNLESVLTYEGTPEVHQLVIGQALTGHNAFR"
     gene            complement(3934..4416)
                     /locus_tag="VLC50_06510"
     CDS             complement(3934..4416)
                     /locus_tag="VLC50_06510"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HSF27156.1"
                     /translation="MAIETLADALQREHLEIDAGIEAFTTGLATGERRIEPLTRAIAA
                     LRRHIYLEEEFLFPPLREAGFAAPILTLVGEHVRMWDLLGLLELELADGTDDAGLRKT
                     SRALMAALHHHNPREERTLYPSAEQVLTAEAGDKLRSFLAAGVLPDGWVSGASARRAR
                     "
     gene            complement(4447..>5031)
                     /locus_tag="VLC50_06515"
     CDS             complement(4447..>5031)
                     /locus_tag="VLC50_06515"
                     /inference="COORDINATES: protein motif:HMM:NF021465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2182 domain-containing protein"
                     /protein_id="HSF27157.1"
                     /translation="MMLPAVAPVASLYVRTIPDGSHRRLLGFVAGYLLVWTASAVPAY
                     ALSLLVMHQISPHPTAARLTAAGIFAVCGVYQLTGLKDRCLRHCRSPLGQLMRYASYD
                     GPGRDLRVGLHHAAYCLGCCWALMLLLFAFGTMTLLAALGLCVLVVVEKLAPAGRQVS
                     RLIGVAALGLAVVALFVPELAPGLAPMMPSPGGM"
BASE COUNT          724 a         1764 c         1835 g          708 t
ORIGIN      
        1 cgtggcatcg cggcccggtt cgccgtcgcc gacgcgctcg ccctggcgca gctgggcgag
       61 acgttcgaca ccgccatcga cagcggcacc ttccacctct ttgccggccc ggcgagatcg
      121 gcgtacgtcc agagcctgag caccgtcctc aagctaggcg gtcggcttta tctgctcgcc
      181 gcccgggacg gcgggtccgg tggctgggga ccgccgggcc tgagccgggc cgagctggtc
      241 gccgccttcg ccggcgggtg gcggctcgag gcgctggaga gcagccggta tgacgtgacc
      301 gccgccgcgc cggtcgacca cgtcgatgcc tggctggcca cgctggcgcg gacccccgcc
      361 cgcgaggggg atgtggcagc cgactagacc gagcgttggt gtggcgccga gcgcccgggt
      421 ggcgccgacc ggacacaaag gacggaacaa cgccggccta cgacgccagc tcgtgctcac
      481 tgggctggcg gcggcacgct tgctccgtcc ttactgtccg ccccggtcga gctcagccag
      541 ccggaccggc gcacgagcgc atgatcagcc gggctggtgt cagggcgtga cgatcagctg
      601 ctgccaccag cggtccagcc agccgcgcag gtcggccagc cgctggtcgt cgaggcgctg
      661 ctgctggttg gcccggcgga acgcgtgccc gagctcggtc agtgcccggc ccacctgctc
      721 tatcgcaaag tcgaccgcga gcgcgcagcc ctcgtgccgg ccgcaccccg gcgtcaccac
      781 cggctcggag cagcagcccg ccgccagctc ggcggtaaac gcggccagcc agcgcggcgg
      841 cggggtctcg gcgaagcgca tcgtcagccg cacggtggtc tcgtcgacca gatcgagggt
      901 cggcaccgct tcggggccat atcgctgcgc caccgtgtcg cccctctcct agccgccggt
      961 gttctcccag cgccagtccg gcagccgtgg gcgctcgccg agtgcggcgc gcccggtggc
     1021 ccacagcagg gtgggccatg gttcgtgacc gcggggagca tatgggaaca ggtgagccag
     1081 cactcgggcg ctcaggtcgg gcgccggcgc ccaggcgaag cccaggcccc ggctgaggtc
     1141 ttcggtgtgc accaacagct cgacgatgcc catcgccgcg aagtcgcacg ggccggccac
     1201 gccgtacggg tgccagctgc gcgtgcccgc atccgccgtg cgtgcgaccg agctgagcag
     1261 tccgccggtc gcccgcacca gagcgcgcag ctccgccggg ttgggcgtgc cctcgaggac
     1321 aaggtccaac gcggcatagc cgtgagcgcg cggtgcggtg agctgggtcg cgtagccgat
     1381 gaagtcgctg gcgatgtgca ggaccgtggc ccgacagtcc cagccgaggt cgccggcagg
     1441 tcgcgaccag tccacggcgt ctgccgccgc catcgcctcg gtggccgcca cgatggcctc
     1501 gtcgagtgcg ctcgcccagg cggttccatc gagctgcgtg gtcatggctt caccctgacg
     1561 atctcgatga ccgacgagcc ggacaggatc tctccggccg agtcgatcgc gaagttgagc
     1621 cggtagagac caggtgccgc gtcggcgggg atgaggattg tcgaggtcag cgccttgccg
     1681 ccgtgggtgg tcgtccgtgc ggacgtcgcc tgtgcaaacg gcgccgcggt ggtggcggtc
     1741 ttcagccgat cgacggtctt gaacgggcca gcaagctctg cggtcagcat caccggggtc
     1801 gggggccggg cctccgtcat cggcagtgcc tcactgatcc agtgcaggac gaagagatcg
     1861 ccgggatgcc agggcctttc gatgtcggat gcctggcaac aggtgtagcg gtaggtggcg
     1921 ggcacaggcc cgctgacagg ttcggggctc gctgtgcatg cggtcgtgag cggtacggcg
     1981 agcatcatca gcgcgattgc cccgcgatgg gccacgtgca tgtgtcaccc ccgtaggtca
     2041 agccctatca cgagctcgcg cggtccccgt ctggagcgca cagctcggcg tggcgcggct
     2101 cgagcccggc gatcagcgag gtgagcgcca gctcgaagct ctcggcgtcg atccgctcgg
     2161 cgtcctggcg gatcaggtgg gccttgacca ggtggggata gcgggccagg tagacctcgg
     2221 tgtcgtcggc gaacccgctg ccgaacggcg tggtcgccgc gccgatcacg aggtatttca
     2281 ccgacgcggc gatcatcgtg gcctgccgtg gcggccagcc gtggcgcagc aggccgccgt
     2341 gcacggcgtc ggccatggcc aggaagtcct cccggcgccc ggtgccggcc gcgacgaccg
     2401 gcgccgcgcg tgggtgcgct cgcagcgacg cgtggtagga gcgcccccag acccgcagcc
     2461 cctcctgcca gctgcgcgtg aacccggagg tgtcgacgtc gcgggtgagc aggttggcca
     2521 ccgcgtcgag caccgcgtcc ttggtcggga agtggccata gagcgaggcc gcctgcacgc
     2581 cgagctcgcg ggccagcccg cgcatcgaca gcgcgtcgag cccgtgggcg tcgatgagcg
     2641 cgagcgcggt gtcgcggatg cgctcgcggg agagcagtgg gacgccgggc ctggccacct
     2701 aacgatgtta ggttatgtac gacgttcccg cgtggaagga cccgacgatg gccgcacccg
     2761 tgtcgcccct gggcttgttc ggcaccgacg agctggtcgg tgaggacgac cgggcgatcg
     2821 ccgagaccgt ccggcgcttc gtggacgacc gcatccgacc gcacgtggcg cagtggtacg
     2881 acgcggccgc gatccccgcg cgcgagctgg cgaaggagct gggctccctc ggcgtgctcg
     2941 gcatgcatct gcatggctac ggctgcgccg gcaccagcgc gaccgcctac gggctgacct
     3001 gcctcgagct cgaggccggt gactccgggc tgcgttcgct ggtctcggtg cagggctcgc
     3061 ttgccatgta tgcgatctat gcgcacggca gcgaggagca gaagcaggag tggctgccgc
     3121 gaatggccgc cggcgaggcg cttgggtgct tcgggctgac cgagcccgac gcagggtccg
     3181 accctgccaa gatgcgcacc cgggcccgcc gcgacggcga cgactgggtg ctgtccggca
     3241 ccaagatgtg gatcaccaac gggtcggtgg ccgacgtcgc gatcgtgtgg gcgcagaccg
     3301 acgagcgcat ccgcgggttc gtcgtaccga cgaacacccc tgggttctcc gctccggaga
     3361 tcaccaggaa gctctcgctg cgcgcatcgg tcacttccga gctggtgctc gaccaggtgc
     3421 ggctgccggc ctcggcggtg ctgcccgggg cggtcgggct gtccgggccg ctgtcctgcc
     3481 tcaccgaggc gcgcttcggc atcgtcttcg gcgccctggg ggccgcgcgt gactgtctgg
     3541 agaccgcgat ctcctacgtg cgtgaccgcg aggtgttcgg caagtcgctg gccgggtttc
     3601 agatcaccca gaccaagctg gccgacatgg cgctcgagct gggcaagggc atgctgctgg
     3661 cgctgcacct gggccggctc aaggacaccg gcacgctgcg cccggagcag gtcagcctgg
     3721 gcaagctcaa cagcacccgt gaggcgatcg ccatcgcccg ggaatgccgt gcattgttgg
     3781 gcgccgcggg aatcacgctc gagcacccgg tgatgcgcca cgccaacaac ctcgagtcgg
     3841 tgctcaccta tgagggcacc ccggaggtgc accagctggt catcgggcag gcgctgaccg
     3901 ggcacaacgc gttccgctga tcgcgcgacg cgctcagcgg gctcgccgtg ccgaggcgcc
     3961 gctcacccag ccgtcgggca gcacccccgc ggcgaggaag gaccggagct tgtcgccggc
     4021 ctcggcggtg agcacctgct cggccgacgg atagagggtc cgctcctccc gcgggttgtg
     4081 atggtgcagc gccgccatca gcgcgcggct cgtcttgcgc agcccggcgt catcggtccc
     4141 gtcggccagt tcgagctcca gcaggccgag caggtcccac atgcgcacgt gctcgccgac
     4201 cagggtgagg atcggcgcgg cgaacccggc ctcgcgcagc ggcgggaaga ggaactcctc
     4261 ctcgagatag atgtgccggc gcagcgccgc gatcgcgcgg gtgagcggtt cgatccgacg
     4321 ctcgccggtc gccaacccag tcgtgaacgc ctcgatcccg gcatcgatct ctaggtgttc
     4381 tcgttgcagg gcatcggcga gtgtctcgat cgccattgcc cgaccctagc cacagccgag
     4441 gtgtcgtcac atcccgcccg gcgacggcat catcggggcc aggccaggcg cgagctccgg
     4501 cacgaagagt gcgaccacgg ccagcccgag cgcggccacc ccgatcagcc gcgagacctg
     4561 ccgacccgcg ggggcgagct tctccacgac caccagcacg cagagtccga gcgcggccag
     4621 cagggtcatg gtgccgaacg cgaacagcag cagcatcaac gcccagcaac agccgaggca
     4681 gtaggcggcg tggtggaggc cgacacgcag gtcgcgcccc ggtccgtcgt acgacgcgta
     4741 gcgcatcagc tgccccagag gtgaccggca gtggcgcagg caccgatcct tcaaccctgt
     4801 cagctggtag acgccgcaca ccgcgaagat ccccgccgcg gtcagcctgg ccgccgtcgg
     4861 gtgcgggctg atctggtgca tgacaagcag cgacagtgcg tacgccggca ccgcgctggc
     4921 cgtccagacc aggaggtagc cggccacgaa ccccagcagg cgccggtggc tgccgtccgg
     4981 gatggtgcgc acgtagagcg aggcgaccgg ggccaccgcg ggcagcatca t
//
LOCUS       DATIBB010000169        29071 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_892911, whole genome shotgun sequence.
ACCESSION   DATIBB010000169 DATIBB010000000
VERSION     DATIBB010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 29071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29071
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_892911"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..866)
                     /locus_tag="VLC50_06520"
     CDS             complement(<1..866)
                     /locus_tag="VLC50_06520"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSF27158.1"
                     /translation="MTSTSSTYLQTGTHEYRRASLVLFLAGFMTFAMLYVVQGILPAV
                     SADFDVSPAVSSLALSLTTLALAVAVVVAASWSEGRGRRPLIVGALLGGALLTILAAA
                     SPGFGWLLALRVLTGLVLAGLPAVAMAYVAEEVAPASLGTAMGLYISGTGLGGMSGRL
                     VGGLLASAVSWRVALGVLGLLCLIGGTWVARRLPRSRHFVAVPGGMRARLAGLRTPLR
                     DPVLVRLSLCGFVLMGSLVSYFNYLQYRLAAAPFGLPAGLVALVFLLYLFGTVSSNWM
                     GRLTDRRSRRSVL"
     gene            complement(863..2044)
                     /locus_tag="VLC50_06525"
     CDS             complement(863..2044)
                     /locus_tag="VLC50_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017982725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HSF27159.1"
                     /translation="MTGAVGVGGLTDEERAVVEVVAEFVDREVRPTARELEHANAYPE
                     RLIARMAELGCFGLGIPAPYGEVAVSTACYALVVEELSRGWMSLGGAIGGHTVVSHLL
                     TAYGTPEQRERYLPRMATGELRATMALTEPGGGSDLQAMRTFARRDGDAYVVDGAKMW
                     ITNARRSGLIALLCKTDPAAQPPHRGMSILLVEPAHGYTVSRDLPKLGYKGVESCEVV
                     FESVRVPGDALLGAQEGRGFPQMMRGLEVGRINVAARAVGVARAAYEDALRYAQERES
                     FGKPIWTHQSIGNYLADMATKIRAARLLTLDAAAVVDSGERADLEAGMAKLFASEACM
                     QVALDAVRIHGGYGYSAEFDVERYFRDAPLMIVGEGTNEIQRTVIARQLVERDRARRR
                     Q"
     gene            complement(2041..2571)
                     /locus_tag="VLC50_06530"
     CDS             complement(2041..2571)
                     /locus_tag="VLC50_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009738256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HSF27160.1"
                     /translation="MDARPADGRFFEDFAVGDVYRHPLGRTVTSTDNIWFTLLTQNTA
                     KLHFDHHYAAQTAFGQPLVNSTFTLALVTGQSVVDVSQNVMANLAWDEVRLPHPVFEG
                     DTVYSWSEVLAARESRSRPDVGVVTVRTTGYNQDGVVVCTFARTLLVYKRAHAPQTTP
                     PPLPGASPAPSSPAQP"
     gene            2699..3388
                     /locus_tag="VLC50_06535"
     CDS             2699..3388
                     /locus_tag="VLC50_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020516483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HSF27161.1"
                     /translation="MTASFRRPPTAQEAVLAQLRQWIAVGRLRPGDQIRQDALAAELG
                     VSRVPLREALKILEGEGQITYVARRGYFVTRLSLDDLLEVYRIRELLEAEAVRLSVAA
                     LSSEGLQQLELAGEQVVEAAAAGDMAAMTAANRRFHLALIDSCGMPRLIRLIEQLWDA
                     TEVYRLVYFSEQAHRDVAVAEHRAVVDAVRAGDSEAALAVLGHHREHAIEALRPVLRR
                     LDEAAAAGTVG"
     gene            complement(3421..3972)
                     /locus_tag="VLC50_06540"
     CDS             complement(3421..3972)
                     /locus_tag="VLC50_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019063489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HSF27162.1"
                     /translation="MPRLPPAQLTPAQRQLYDEIANGPRAAGPFALTDERDALLGPFG
                     AMLLSPDVGAALQALGAAVRYRSALTPRIREMAVLVVAAHWRSDFERHAHSAVGRLVG
                     LSEAELAALQDEAPLELADPAEAVALATVRALVDDGDLDDAGYGRACQALGERGVFEL
                     TTLVGYYATLALQLRVFRVPAPN"
     gene            4053..6227
                     /locus_tag="VLC50_06545"
     CDS             4053..6227
                     /locus_tag="VLC50_06545"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012297721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HSF27163.1"
                     /translation="MTTEAATTEAATTEIVASAVPERLRAEAAAVLVRLVGDEQAAFR
                     DGQLEAIHALVEGGRRVLVVQRTGWGKSAVYFVATALLRERGLGPTVIVSPLLALMRD
                     QVEAARRAGLRAVTINSSNATEWAQIAAAVRADDVDLLIVSPERLNNPRFRDEALPGL
                     AASTGLLVVDEAHCISDWGHDFRPDYRRIRDLLRTLPAGRPVLATTATANARVVADVV
                     EQLAATDGSDEVLVLRGSLARESLRLGVLALPTAATRLGWLLAHLDELPGSGIVYALT
                     VSAAEDTAELLARAGHQVRAYTGRTDPDERLALERALRDNQVKALVATSALGMGFDKP
                     DLGFVVHLGAPASPVAYYQQVGRAGRATERADVLLLPAPEDREIWRYFATASMPRRAD
                     AEAVCRALAGASSPMSTTRLETVADVRRSRLELLLKVLDVDGAVRRVSGGWVSTGSPW
                     VYDEARYARVAAAREAEAAAMLGYQAGAGCRMRFLQDQLDDPAAGDCGRCDACAGPWF
                     PAGVPEQEVEAAQTALRRVGVELEPRSQWPAGMSRLGVEVSGRIDVGERLEPGRAIAR
                     LTDLGWGQRLRALLGPGAPDAPADAALLTTCVQVLREWGWSTRPVAVAAMPSLRHPAL
                     ITSVAAHLAQVGRLVDLGTLALAHDDPVGSSGGNSAFRLAGVHDRFVVPPAMAETVSR
                     LTGPVLLVDDLVDSRWTLTVAGRALRRAGAAGVLPFALALAA"
     gene            complement(6216..6356)
                     /locus_tag="VLC50_06550"
     CDS             complement(6216..6356)
                     /locus_tag="VLC50_06550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF27164.1"
                     /translation="MLQGRGPWPGNDNGRGVDLDLLAETVAAVPVEDYLASYEQARDS
                     RG"
     gene            6563..14140
                     /locus_tag="VLC50_06555"
     CDS             6563..14140
                     /locus_tag="VLC50_06555"
                     /inference="COORDINATES: protein motif:HMM:NF027952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HSF27165.1"
                     /translation="MVAKVRRRAVKILAWSCSTLLAAALLVAAPLALAPATAANVSPP
                     CKCIGDYVAPKTIEPTVADVEAPDDDGYSQNKAYRVAATTSSLQVIRQADNQTILKLP
                     RPTQWGFSPDEQRFVTVSTQAMSLYNLADGSGSRLYESTNPQDENWGFSPHGKYLSSF
                     SLPNQVPTLQVVDARTGNVVRLGGSSTATGYTDIGFSPSDDSLLAFSNSGATLYRLSD
                     GVAVYTNTDPPSAYGFSPTGGYLEIKVVTNTTHVELSVVATVDGHPVHTNAYNFAVGA
                     AVQQEASGFSPDEHTFLDAYVNGTDTASLEIVNLRDGRRVFSSSAIDRGSTWSFSPCG
                     GEFAMAVPNGSRQTLTLRATSDGRTLGSALSVPVTALTIEATDDDNTVTYVDATGTQT
                     ITLAPNLWCGAATINHDNDVSGGDSRTWSIELDRPAHDYGVTFRLSSSDSHLTVPASA
                     TIPAGQTTVSFDVTSSVPPEDVLVTVTVAHDEVLGGTEASTFQVSIRGPVGLESLYLG
                     TGEMSGPGTDTGSVYLVASAPTGGTTVSLASDDPAVSVPDTVIVAAGTDSATFPITVA
                     AVDHTTTATVSATSGETTVSATYLVDPIVTTSTRLVVRPGTSTVVGEQVGLDATVTPI
                     EPPADDQDWVSYYGTPTGTVTITDDGTELTSIDVTPPDFGGAEGQVTASWSIAALDAG
                     QHIITVTYSGDDHYGPSQASATVTVREGLSWTGNGGDGRWSNPANWSPSRVPDAQNPD
                     IVVISSATELHLDDDLPGLELSGLHLSGPITLDGQAIILDGELAATRSGATIGNDISL
                     TIDTHNFYADTMLRLDGVVSGAGGPIASTASGGFVVLAGAANTYTGETRVSEGATLMA
                     GADQALGARGASQGAEVYGTLCISRGVSLLEPVASHGGNVASCGTPDDLSATLADDPA
                     DVGSPTTATMSGSLLTDGTTGLGPLSCYADDPTGDELDLNVDGAISGSGQVSIGCPGR
                     RDGPSMGSVSFGGEQPNTYTGGTQIFGTLTLAKGNGVPAAAGTLVAANGGTLKLAGDD
                     QIGHSAHADVNILDAGPYHATVADLAVGVLKTVVDAAGHSGGVTVLGPTQLNSVQVSV
                     ASDPAIGSTFTVVDNTSSDPVGGCTNGVVPGDEATSYRIDCTGGDGNDVVLTTVWHPT
                     VTLTTDPMDGAVSGSPVTVTATVSSPSGAAEATGQVSLKTFHFNYGWIYQATAATATL
                     VDGVATFSFPDTSFLASGNGLVASYLGDSQHAPVNSRLMRYLSWPDPTGIPLPAAPVN
                     LSAGVSGPGGSSPNTAFLSWGYPPGTTYTDYVSYHITVAPDHGEPYVVDTNTAQSDDD
                     LIYRDFPGFPRYRLSGLQPGETYTFSVQAVNHAGAGPASNAVTVTVPVAPAPGAPTDL
                     SAIAGPGQAQLAWTAPADDGGAPINGYEVTPVNDGVRQEAIRTADATTHLTVTGLTPG
                     ASYVFTVAAVHVGAVGPSSSPSVAVTPTAISAAAVVPGVPSDVVATPGPGSLTLTWSP
                     PADDGGASIVHYVIVPSGTAGSRMQLVTSGPATTVTLSGLPDDLMTFTVAASNEAGTG
                     PASAPTAPVRPVGAVPGASTDVLVSAGAGQVFVTWTPPADEGGSPIVRYLVTPYLAGA
                     AQAPVATAGNGTSATVAGLLSDVPYTFTVSAVNAAGSGPASEQSAPVRPLSAPPIVSV
                     PTAPETFTGSVDGQAVTLTWAPPSSNGGSAVAGYLVEAFKYAYSNYGEWGGGTYQGGP
                     YQSWWLPADATSFNLTTDTISWPWTSGAYRFTVSAANDIGVGAQASPWFSAPAIWVAP
                     PTEPGSGGGLPPEPTVPPGQNGDVSNGAGAGVHGSGFGPDEPVSATLHSTPVDLGTLT
                     AAADGTVTGTVTIPADTPAGDHTIQLVGQNTGHVVDIPISVHQALSIGDITSSSMTVS
                     WPSAPEGATGLTVSVDGSPVTTLSTTVSSYTVTGLSPATTHTLSVQASSGDSSLGELA
                     IIGTTLSTLEASAAKTAVSLSWLASSDPTVTGYRVMRDDNADGDFDVLADVSGPSFTD
                     FDLRASTTYRYHVLAVHADGSTSVWSESVAATTPDLAISNVSFSAPTALGGSNAAYGS
                     TLDIEVTADAGRTASAVVSGQAPDGSAVSKTVALSETSSGAYDGTLPLNTTIATIDAI
                     TATLSDGVHSVDAEATGLPLAQSGFLSIEISPSNVDVAGAQLELVNTALGVDEMRAVV
                     APAHVGIAVAAGSWTLRLLAPDGDVLAERADVAVSNATMSSLSLAPVRHASLTVGLVA
                     PDGETSGGFTVTVTDSDGAVLGQTDVASGDSSVEFDGLVAGTAVTVTATLDDASRPLL
                     SAVSTTSTLAGGANTVSLVEQALSTGRIQGLVTPAATVVVDQQVDGRSWQFTTTTDSA
                     GHFGIDVLAATAHVTASADGYLPATFDIDVAPSTTVTASPVALEPVGNSRVATRLTID
                     PISSPRDLMNLIFGRYKIQAHLVADDGSPIKGARVVIDSWKGPFCVAETHASGVASCD
                     VPPSLRSVVLLGQVHASYAGSDKYLPSSSGDIVGPGWHGKDHLAA"
     gene            complement(14129..14578)
                     /locus_tag="VLC50_06560"
     CDS             complement(14129..14578)
                     /locus_tag="VLC50_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015099324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HSF27166.1"
                     /translation="MRSDATTAEAYLAGLAPEPREVVAQVRQLVLANLPDGYEETMAW
                     GMLCWVVPLERYPETYNGQPLGYVALAAQKRYYSLYLMTLYPDGEREREFRTRWQAGG
                     RRLDMGKSCLRFRRVDDLDLDLLAETIAAVPVEDFLALYEQARDSRG"
     gene            14652..15281
                     /locus_tag="VLC50_06565"
     CDS             14652..15281
                     /locus_tag="VLC50_06565"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HSF27167.1"
                     /translation="MPSSPRRAAPLPPDARRAALVAATLPLLRAHGPHLSTRQIAEAA
                     GVAEGTLFRVFPSKDDIVAAAIAHAINPARTAAELAAIDRSLPLTPRLVAATRVIQRQ
                     LDGIIALLTLRRDCAPSDSDHLPDHHAGTVVVLAGLAHLVEPDRDALRVEPAELARRL
                     RLLTIAASHPHLTDDDPMSAEEIVATLLDGLRGPRRSHASTAHDGSTGC"
     gene            15275..17008
                     /locus_tag="VLC50_06570"
     CDS             15275..17008
                     /locus_tag="VLC50_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018786108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSF27168.1"
                     /translation="MLTGLLRTYLRPYRGPLAAVVALQLVGTMASLYLPSLNADIIDN
                     GVAKGDTGYILMIGGWMLTVTLVQIGCSIAAVYLGAQTAMSFGRDTRAAVFHRVGTFS
                     AREVNQFGAPSLITRTTNDVQQVQMLVVMSATMMVAAPIMCVGGVIMALRQDVGLSWL
                     ILVSVSVLVVLIGAIVSRMVPQFRLMQTRIDAVNRVLREQLAGIRVVRAFVRERHETT
                     RFAGANTELTDTALRTGRLMVLIFPAVMLVFNASSVAVLWFGAARVEHGQMQVGALVA
                     FLSYLMQILMSVMMATFMVIMIPRAAVCAGRIGEVLDTESSVRPPAAPVTQLTRQGEL
                     KLRNVEFRYPGAAEPVLRDITFAARAGQTTAIIGSTGAGKTTLVSLVPRLFDVTAGSV
                     LVDGVDVRELDPDLLWSRIGLVPQRAYLFSGTVASNLRMGDPQASDEALWAALEVAQA
                     REFVEAMPGGLDAPIAQGGTNVSGGQRQRLSIARALVRRPEIYLFDDAFSALDLATDA
                     RLRAALQPLTADAVVLIVAQRVSTIRDADQIVVLEDGAVVGLGTHDELLTSCPTYVEI
                     VASQHAAEEAA"
     gene            17005..18960
                     /locus_tag="VLC50_06575"
     CDS             17005..18960
                     /locus_tag="VLC50_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSF27169.1"
                     /translation="MSATTRVPRRPHPGHGPFAAAGAPTDKAMTFGPSARRMLARLRP
                     HRTSVVAVLALAVASVALSVVGPKILGRATDLIFGGLIGAQLPAGTTRDQAVAAARDA
                     GNTNFADMLARMAVTPGQGIDFTALSQVLSFVLVLYLAASVLGWLQGYLLNDVVQATV
                     FRLRADVEDKLHRLPLRYVDQQPRGELLSRVTNDIDNIQTTLQQTLSQLLTSLLTVLG
                     VLVMMFVISPLLALIALVTIPLSMVVTARIAQRSQPLFIAQWTHTGALNAQIEEAFTG
                     HELVKVFGRQREVEAAFHAKNDELFRASFGAQFVSGIIMPTMMFIGNLTYVAIAVVGG
                     FRVATGAISLGNVQAFIQYSRQFTQPLTQVASMANLLQSGVASAERVFELLDAPEQAP
                     DPAAPARPERLRGRVELEHITFRYEPDQPLIDDLSLVAEPGHTVAIVGPTGAGKTTLV
                     NLVMRFYELDGGRITLDGTDITTMRRDELRSSIGMVLQDTWLFGGTIRDNIAYGRPGA
                     SEDEIVAAARAAYVDRFVRALPEGYDTVIDEEGTNVSAGEKQLITIARAFLADPALLI
                     LDEATSSVDTRTEVLVQHAMAALRADRTSFVIAHRLSTIRDADTILVMEAGRIVEQGT
                     HEQLLAAGGAYARLYSAQFSGALAETS"
     gene            complement(18918..19241)
                     /locus_tag="VLC50_06580"
     CDS             complement(18918..19241)
                     /locus_tag="VLC50_06580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF27170.1"
                     /translation="MIVRQWRGWVAPERADDYAAYMAEVAIPAYAGAAGNRGAWMLRR
                     SDGERVEFCALSMWESIEAVTAFAGGTPDRAVFYARDDDFLLAREWTVRHYEVSASAP
                     LNWAE"
     gene            complement(19254..20039)
                     /locus_tag="VLC50_06585"
     CDS             complement(19254..20039)
                     /locus_tag="VLC50_06585"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HSF27171.1"
                     /translation="MTEMRNYPSGAPCWVDLGTTDLTAAQEFYTRLLGWSYVDTGEEG
                     GHYQLAMLKDRPIAGMMAMSPEMLETAVPPSWTTYFASDNADDVGKWVTEHDGAVLMG
                     PMDVMTQGRMLVGRDPAGAVFGVWQPRDMPGSGLLGEPGSVAWTELLSRDPQATGEFY
                     AAVFGFDRETQDIGMTEPYTVFSIGETQVAGMMQMPTDVPPEAPSYWMTYFAVTDADA
                     AVATVREMDGTVLAEPRDTEYGRWAMIADPQGAVLSVLALPAS"
     gene            20206..22683
                     /locus_tag="VLC50_06590"
     CDS             20206..22683
                     /locus_tag="VLC50_06590"
                     /inference="COORDINATES: protein motif:HMM:NF025011.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-associated domain-containing protein"
                     /protein_id="HSF27172.1"
                     /translation="MSGSFAGWLRGLDEARLVRLLELRPDSCGPPAPESMEALAERLS
                     SMQSVHRALGELSELALVVIKVVQGLGGSCSRAEIDEHLHLPSPADQAAVELAVAALV
                     ARGLLLDRDEALPRQGGGPDGLEGGLTLVKTVRALFPGPFGLGRPLRQLLGATSPSWL
                     AQMRTTYRLPEAGRGETHTALVALLTRRRILDEVRHGPPRLTELLHDVMADGGIVVLE
                     GSGTFSLDVSQRTAGTDDRLTYAVDHALLVPVDWQVLELPNEVALALRSPGWALSAPT
                     SPPEPPTAATDHATVERESGASGAAAVIVVTDLLELLSATPARELKTGGVGIREVRRL
                     AKALGRSEADVAMALQLVIAARLVGVTGLEVVPRAEYDEWRMRTPAERLASLLSAWWS
                     RPGWVSGELDDDDKPLPPVSPRRADERVLALRQAMVGVLAALPAGRGAVDAAGAAATV
                     AWSRPLGFPQGQQLTAAAQATWWEGHWLGALAHGRASAVGAALTSYDEDRLLQACQVL
                     VPEAISQAMFQADLTAVVAGSPSSEVASLLDSCADREASGGAVIWRFSPATVRRCLDT
                     GQSAPALLRALGAIARDTLPQPLTYLVNDMARRHGRIAVRPLRCVLVSQDAALLAELA
                     AHRALRPLALSVVAPTVLASAKSLDETVTLLRQAGYAPVRHTQTGELVVDRATPRRVK
                     VTPRPTATTRAPRPGEAAPTEPVTPAALAKALREASRHPGTPAPGKARPAPPAPPRPA
                     SSAASTLARLAERAGHLSDDERSLLAHAIDFGGKVGIDYRDVHGSFSTRAISDVALDP
                     PYLEAFCHLREDDRVFLLSGISAVYPA"
     gene            22710..23927
                     /locus_tag="VLC50_06595"
     CDS             22710..23927
                     /locus_tag="VLC50_06595"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014741642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HSF27173.1"
                     /translation="MLERRLPRWAEVRELLHPHAPTFDNTARRLATVASISDLRRLAR
                     RRAPRAVFDYTDGAAGDEASLRRSRAAFASVEFCPSVLHDVSTVDTSTTILGAPSALP
                     LVFAPTGFTRMMHAEGEPAVARVAGRTGIPYALSTLGTTSIERLAADAPDTRRWFQLY
                     LMRDRGAGRDFVARAQAAGYEALVLTVDTPVAGPRRRDVRNGLTIPPAPTLRTVAEGA
                     LHPGWWYDLLTTEPLEFASLNRFEGTVAELIAKTFDASVTMADLTWLRETWKGPLIVK
                     GVTSPADAKAVVDHGADAVVVSNHGGRQLDRSPTPFEVLPEVVAVVGDRAEVYVDGGI
                     LSGADVVAAVARGAKAALVGRAYLYGLMAAGERGVEKAADILATEIANTMALLGVTSV
                     GELGPEHVRIRPA"
     gene            complement(23928..24329)
                     /locus_tag="VLC50_06600"
     CDS             complement(23928..24329)
                     /locus_tag="VLC50_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain nuclease"
                     /protein_id="HSF27174.1"
                     /translation="MLLVDTSVWVDYLRGRDSVASAALREAITDGRALATTEPVVMEL
                     LAGAADDVAVDRLERLTGGLTMLSVQPALDFTAAATLYRAARRRGVTVRRLIDCLIAA
                     IAMRHGVALLHKDIDFDAIAAFTDLEVVSAR"
     gene            complement(24333..24554)
                     /locus_tag="VLC50_06605"
     CDS             complement(24333..24554)
                     /locus_tag="VLC50_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019283586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HSF27175.1"
                     /translation="MGRTNIEIDDELVAAVMRRFGLASKRAAVDLALRRLVGPPLTPE
                     FLRDLEGSGWEGDLESMRSSRASDEAAGE"
     gene            complement(24571..25725)
                     /locus_tag="VLC50_06610"
     CDS             complement(24571..25725)
                     /locus_tag="VLC50_06610"
                     /inference="COORDINATES: protein motif:HMM:NF025200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4192 domain-containing protein"
                     /protein_id="HSF27176.1"
                     /translation="MSSQPRPVVKFSSPTDIVGVVPLRVGFVPTESIVVVCLHGPRRR
                     AGLVMRHDLVPIEQLEELADVLAWHVRRAEADGVILVVYTDAPDVDGSLPQHEFVDDL
                     VDRLGIEVVECLLVRQGRWWSYSCALACCPPEGTPIPSEPQGPAAIVSAAAAFDGRAV
                     LADRAALEASIRPRRGPLSDGARMLAFDQAAQAVIAAAEDGADALTAETMALLESAAS
                     RWAAGERDLAPVEAARIVLGLSSKWCRDYAATLALDLDDDAYLSFLTELCQRTDDEWA
                     APVCTVLAWVAYAHGDGALANVAVDRALAAEPDYEMARLLLRGLQGQMPPAEVRALLG
                     NVRRDMEAIARGETPSFERELARRRGSRSRQPPLRQARRTRAARRRRKAR"
     gene            25862..26869
                     /locus_tag="VLC50_06615"
     CDS             25862..26869
                     /locus_tag="VLC50_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013421598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HSF27177.1"
                     /translation="MDLVELTPSREQYAYTFGGVAPLMTIKPGTALRLWSDEAFGGVL
                     RSVDDLSSARVDLRFVNPQTGPFWVDGAQPGDTLALHFVALEPARDWGASAAIPFFGG
                     MTSTDRVVTLQDPLPDTTWIYELDRARNTVTFAARHSEHRIELPVEPMLGTVGVAPAG
                     GEARSSLVPDRFGGNMDTPQMRAGSTVFLGVNVEGALFSIGDGHYRQGEGEACGTAVE
                     GAMTTTLIVELIKGGAPGWPRIEDDTHWMTVGSSRPLEDAWRIGNAELVHWVAELYGL
                     HPMDAYQLCSQVAEVPIANVVDANYSVVVKAAKALVPAATAFDGLHAELRERAASLGS
                     V"
     gene            26909..27667
                     /locus_tag="VLC50_06620"
     CDS             26909..27667
                     /locus_tag="VLC50_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HSF27178.1"
                     /translation="MDLQLGGKRVLVTGGTRGIGRAIVEAFLDEGAVVGFCARKPDEV
                     AATESALTSRGTVRGTVLDVGEGPALAAWVSETAEAFGGLDVVVANVSALAIPDTDEN
                     WETSLNVDLMHTVRLVKAAMPHLERSDAASIVAISSVSGRESDFASGPYGTAKTAIVG
                     YIHGLALQLAEKGIRANCVSPGNTYFEGGVWASIEAGDPALYEMAVGLNPTGHMGTPA
                     EAAAPVVFIASPRASRISGANLVADGALTRGIQL"
     gene            27695..28585
                     /locus_tag="VLC50_06625"
     CDS             27695..28585
                     /locus_tag="VLC50_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014926816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HSF27179.1"
                     /translation="MIISGAEEALRQRIMASLAERTAHNGGVISRAELSDFSFAPGER
                     RRLIDTSRGIWNPRDLAATLTIVSSPNGPYDDQEVEGGLFRYDYRAGSDHGDNTKLRR
                     AAELGLPLILLRKIAPGVYVPIFPVYVVSDDPSRKQFLIALDESLRFLRDPLHPTAAE
                     RRYAERVVRQRLHQPEFRGRVIRAYETQCAICRLRHGELLDAAHILADADGGLPVVTN
                     GLAMCKIHHAAYDRDFLSVDRDYTIHIKPSLLEERDGPMLLHGLQEVHGHTIEVPHAK
                     AERPDPTLLDARHRVFLASA"
     gene            complement(28595..>29071)
                     /locus_tag="VLC50_06630"
     CDS             complement(28595..>29071)
                     /locus_tag="VLC50_06630"
                     /inference="COORDINATES: protein motif:HMM:NF014741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HSF27180.1"
                     /translation="TCDYDLLHRRLTNGVLDTGVKIPAHELRRLACGADLLPAVMGGA
                     STVLDLGRARRLFSTSQRRALELRDGGCTFPGCHHPARACEAHHQDHWEHGGPTDLCN
                     GALLCDYHHDQAHRQGWRSRLSSANGQVEWQPPRVLDRAQQWRQHHRYTLETIRRR"
BASE COUNT         4327 a        10432 c        10046 g         4266 t
ORIGIN      
        1 agcacgctgc ggcggctgcg ccggtcggtg agccggccca tccagttgga gctgacggtg
       61 ccgaacagat agagcaggaa caccagcgcg accagtccgg cgggcagccc gaacggcgcg
      121 gcggcgagcc gatattgcag gtagttgaag taggacacca ggctgcccat gagcacgaag
      181 ccgcacagcg acagccggac cagcaccggg tcgcgcaagg gcgtgcgcaa gccggccagc
      241 cgcgcacgca tcccgccggg caccgcaacg aagtggcgtg agcgcggcag ccgtcgcgcg
      301 acccaggtgc cgccgatcag gcagagcagg cccagcactc ccagcgctac ccgccacgac
      361 accgcactgg ccagcaggcc gcccaccaac cgcccggaca tccccccgag gccggtgccg
      421 ctgatgtaga gccccatcgc ggtccccagg ctggccgggg cgacctcctc ggcgacgtag
      481 gccatcgcca cggccggaag cccggccagc accagcccgg tcagcacgcg cagcgccagc
      541 agccacccga accccgggct ggcggccgcg aggatcgtga gcagcgcgcc gccgagcagc
      601 gcgcccacga tcagcggccg ccgaccgcgg ccctccgacc aggaggcagc cacgacgacg
      661 gcgacggcca gcgccagcgt cgtcaacgac agcgccaggc tggacacggc gggcgacacg
      721 tcgaagtcgg cgctgaccgc gggcaggatg ccctggacga cgtagagcat cgcgaaggtc
      781 atgaaccccg cgaggaagag caccaggctg gcccgccggt actcgtgagt gccggtctgc
      841 aggtaggtcg acgaggtgct ggtcattgcc ggcggcgcgc gcggtctcgc tcgacaagct
      901 ggcgggcgat cacggtgcgc tggatctcgt tggtgccctc accaacgatc atcaacgggg
      961 cgtcgcggaa gtagcgctcc acgtcgaact ccgcggagta gccgtagcca ccgtggatgc
     1021 gcaccgcgtc cagggccacc tgcatgcacg cctcggaagc gaacagcttg gccatgccgg
     1081 cctccagatc ggcccgctcc cccgagtcca cgacggcggc ggcgtcgagc gtgagcaggc
     1141 gggccgcccg gatcttggtc gccatgtcgg cgagatagtt gccgatcgac tggtgggtcc
     1201 agatcggctt gccgaagctc tcccgttcct gggcgtagcg cagcgcgtcc tcataggccg
     1261 cccgtgccac gccgacggcc cgggccgcga cgttgattcg cccgacctcg agcccccgca
     1321 tcatctgggg gaagccgcgc ccctcctgcg ccccgagcag ggcatcgccg ggcacccgca
     1381 ccgactcgaa gaccacctcg cagctctcca cgcccttgta gccgagcttg ggcaggtcgc
     1441 gggagaccgt gtagccgtgg gcgggctcga cgagcaggat gctcatgcca cggtgaggcg
     1501 gttgcgcggc tggatccgtc ttgcacagca aggcgatcag ccccgaccgg cgggcgttgg
     1561 tgatccacat cttggcgccg tcgaccacgt aggcgtcccc gtcgcgacgc gcgaacgtgc
     1621 gcatcgcctg caggtccgat ccgccacctg gctcggtcag cgccatcgtc gcccgcagct
     1681 cgccggttgc catccggggc aggtagcgct cacgctgctc cggcgtgccg taggcggtga
     1741 gcaggtggct caccacggtg tgcccgccga tggcgccgcc aaggctcatc cagccgcgcg
     1801 agagctcttc gacgacgagc gcgtagcagg cagtggacac cgcgacctcg ccgtacggcg
     1861 cggggatgcc caggccgaaa cagccgagct cggccatgcg ggcgatcagc cgctcgggat
     1921 aggcgttggc gtgctcgagc tcgcgcgcgg tagggcgcac ctcgcggtcg acgaactcgg
     1981 cgacgacctc gacaaccgcc cgctcctcgt cggtgagccc gccgacgcca accgccccgg
     2041 tcacggctgc gccggggatg acggcgccgg ggaagccccc ggcagcggtg gcggcgtggt
     2101 ctgcggcgcg tgtgcgcgct tgtagacgag caaggtcctg gcgaaggtgc agacaacgac
     2161 gccgtcttgg ttgtagccgg tggtgcgcac cgtcacgacg ccgacgtcgg ggcgcgaccg
     2221 ggattcccgc gcggccagga cctctgacca ggagtagacg gtgtcgcctt cgaagacggg
     2281 gtgaggcagc cggacctcgt cccaggcgag gttggccatg acgttctggc tgacatcgac
     2341 cacgctctgg ccggtgacca gcgccagcgt gaaggtggag ttgaccaacg gctggccgaa
     2401 cgccgtctgc gcggcgtagt ggtggtcgaa gtgcagcttg gcggtgttct gcgtcagcag
     2461 ggtgaaccag atgttgtcgg tgctggtcac cgtgcgcccc agcgggtggc ggtagacatc
     2521 acccaccgcg aagtcttcaa agaaccgccc gtccgcgggc cgcgcgtcca cctggtgcct
     2581 ccctgactcg aggggccagc gtatacatcg gataatgccc aacgacactg tggtgctctg
     2641 gaggcgcggg atgggacccc ttgaggatgt gcggaccgac gcgcacccag gagggaaggt
     2701 gacggccagc tttcgacggc cacccaccgc ccaagaagcg gtgttggcgc agctgcgcca
     2761 gtggatcgcc gtcggccggc tgcgtcccgg cgaccagatc cgccaggacg cgctcgccgc
     2821 cgagctcggc gtcagccggg tgccgttgcg cgaggcgctg aagatcctcg agggcgaggg
     2881 gcagatcacc tacgtcgccc ggcgtggcta tttcgtgacc cggctctcgc tggacgacct
     2941 gctcgaggtc taccggatcc gggagctgct cgaagccgag gccgtccgcc tgtcggtggc
     3001 cgcactgagc agcgagggcc tgcaacagct cgagctggcc ggcgagcagg tcgtcgaggc
     3061 ggcggcagcc ggggacatgg cggcgatgac cgcggccaac cgacggttcc atctcgcgct
     3121 catcgacagc tgcggaatgc cccggctgat ccggctcatc gagcagctct gggacgcgac
     3181 cgaggtctac cgcttggtct acttcagcga gcaggcgcac cgcgacgtcg ccgtcgccga
     3241 gcaccgcgct gtggtcgacg ccgtgcgcgc gggtgactcc gaggcggccc tggcggtgct
     3301 gggccaccac cgcgagcacg ccatcgaggc gctgcgcccg gtgctgcgcc gcctcgacga
     3361 ggcagccgcc gccggcacgg tcggctgagc gcgagccggc cccgcacgag agcgagtcgg
     3421 tcagttggga gccggcaccc ggaacacgcg cagctgcaac gcgagggtgg cgtagtagcc
     3481 gacaagggtg gtcagctcga acacaccacg ctcccccagc gcctggcagg cccggccgta
     3541 gccggcgtcg tcgaggtcgc cgtcgtcgac aagtgcgcgc accgtggcca gcgcgacggc
     3601 ctcggccgga tcagccagct cgagcggcgc ctcgtcctgc agggccgcca gctcagcctc
     3661 gctgaggccg acgaggcggc cgaccgcgct gtgggcgtgc cgttcgaagt cactgcgcca
     3721 gtgcgcggcc acgaccagga ccgccatctc ccggatccgc ggggtcagcg cgctgcgata
     3781 gcgcaccgcc gcacccaggg cctgcagggc ggccccgacg tccgggctga gcagcatcgc
     3841 gccgaacggg cccagcaggg cgtcccgctc atcggtcagg gcgaacggac ccgccgcacg
     3901 tggaccattg gcgatctcgt cgtacagctg gcgctgggcc ggggtgagct gtgctggagg
     3961 cagtcggggc agccgcggct gggggcaggt cgggagatcc acgccgggga cgatacgcgg
     4021 gctgtcggcg gcccggtcta gcgtccgggc ccatgacaac cgaggcggca acgaccgagg
     4081 cggcgacgac cgagatcgtg gcgagcgccg tgcccgagcg gctgcgggcc gaggccgccg
     4141 cggtgctggt ccgcctggtc ggcgacgagc aggcggcctt ccgcgacggg cagctcgagg
     4201 ccatccacgc cctcgtggag ggcgggcgcc gggtgctggt cgtgcagcgc acgggttggg
     4261 gcaagtccgc ggtctacttc gtcgccaccg ccctgctgcg cgagcgcggc ctcggtccca
     4321 cggtgatcgt ctcccccctg ctcgcgctga tgcgcgacca ggtcgaggcg gctcggcgag
     4381 ccgggctgcg cgcggtgacg atcaacagtt ccaacgccac ggagtgggcc cagatcgccg
     4441 cggcggtgcg ggccgacgat gtcgacctgc tcatcgtcag tcccgagcgg ctcaacaacc
     4501 cacggtttcg cgacgaggcg ctgcccggac tggccgcgag caccggcctg ctcgtcgtcg
     4561 acgaggccca ctgcatcagc gactggggcc acgacttccg gcccgactac cgccgcatcc
     4621 gcgacctgct gcgcacgctg ccggccggcc ggcccgtgct cgccacgacc gccaccgcca
     4681 acgcccgcgt cgtcgccgac gtggtcgagc agctcgcggc caccgacggc tccgacgagg
     4741 tcctcgtgct gcgcgggtcg ctggctcgcg agagcctgcg gctgggggtg ctggccctgc
     4801 ccaccgcggc gacccggctg ggctggctgc tcgcccatct cgacgagctg cccggcagcg
     4861 gcatcgtcta cgccctgacc gtctccgcgg ccgaggacac cgccgagctg ctggcccggg
     4921 ccggccacca ggtgcgtgcc tacaccgggc gcaccgaccc cgacgagcgg ctcgcgctcg
     4981 agcgtgcgct gcgcgacaac caggtcaagg cgcttgtggc gacaagcgcg ctcggcatgg
     5041 gcttcgacaa gcccgacctc ggcttcgtcg tccacctggg tgcgccggcc tcaccggtcg
     5101 cctactacca gcaggtcggc cgggccgggc gggccaccga gcgtgccgac gtgctgctgc
     5161 tgcccgcgcc cgaagaccgt gagatctggc gctacttcgc gaccgcgtcg atgccccgtc
     5221 gcgccgatgc tgaggcggtg tgccgggccc tggctggcgc gtcgagcccg atgtccacga
     5281 cccggctgga gacggtggcc gacgtacgcc gcagccggct cgagctgttg ctcaaggtgc
     5341 tcgacgtcga tggcgccgtc cggcgggtca gcggcggctg ggtctccacc ggcagtccgt
     5401 gggtctacga cgaggcgcgc tacgcacggg tcgccgccgc ccgcgaggcc gaggcggcgg
     5461 cgatgctcgg ctaccaggcc ggcgctggct gccggatgcg gttcctgcag gaccagctcg
     5521 acgacccggc cgccggtgac tgcggccggt gcgatgcctg tgccgggccg tggttcccgg
     5581 cgggtgtgcc cgagcaggag gtcgaggcgg cccaaacggc gctgcgccgg gtcggcgtcg
     5641 agctcgagcc gcggtcgcag tggccggccg ggatgtcccg gctcggggtc gaggtctccg
     5701 gtcgcatcga cgtcggcgag cggctcgagc ccgggcgggc gatcgcccgg ctcaccgacc
     5761 tgggctgggg ccagcggctg cgcgcgctgc tcgggccagg cgctcccgac gcgcccgccg
     5821 acgccgcgtt gctgacgacc tgcgtgcagg tgctgcgcga gtggggctgg tcgacccgcc
     5881 cggtggccgt ggccgcgatg ccctcgctgc ggcaccccgc cttgatcacc tcggtggccg
     5941 ctcacctggc gcaggtcggc cggctcgtcg acctggggac gctggcgctg gcccacgacg
     6001 accccgtggg ctcgtccggc ggcaacagcg cgttccggct ggccggggtg cacgaccggt
     6061 tcgtcgtacc gccggcaatg gccgagacgg tgagccggct gaccggtccg gtgctgctcg
     6121 tcgacgacct cgtcgactcg cggtggacgc tcaccgtggc cgggcgggcg ctgcgccgcg
     6181 ccggggccgc cggggtgctg ccgttcgcgc tggcgctagc cgcgtgagtc gcgggcctgc
     6241 tcatagctgg cgagatagtc ctcgaccggc accgcggcga cggtctcggc cagcaggtcg
     6301 aggtcgacgc cacgaccgtt gtcattgcct ggccaagggc cacgaccttg cagcacacat
     6361 gctgcctgcg gggtcgcaca atggcccccg ccacgtgccg ggaacacgat cacttgatcc
     6421 gggcatatca atgcagattg ccccgattgg cgcacccgat gaaccaccta ttgcgacagg
     6481 gatctagtcg aaggtatgac ttagggtgta tgcctgccat cggcaagggc acacggggga
     6541 cacggggaga ggtgcaggaa ccgtggtggc taaggttcgc cgtcgcgcag tcaagattct
     6601 cgcatggagt tgttccacgc tgttggccgc cgcgctcttg gtggcggcac cgctagctct
     6661 cgctcccgcg acggcagcca atgtgagtcc accgtgcaag tgcatcggcg actacgtcgc
     6721 accaaagacg atcgagccga ccgtcgcaga tgtcgaagct cctgatgatg acggctattc
     6781 ccaaaacaag gcgtatcgcg tcgctgcgac cacaagcagc cttcaagtca ttcgccaagc
     6841 ggacaaccag acgatcctga aacttcctag gccgacacag tggggcttca gcccggacga
     6901 gcagcgtttt gtcacggtat caacacaggc gatgtcgctg tacaacctcg ccgacggcag
     6961 cggctcacga ttgtacgaga gcaccaaccc acaggacgag aattggggat tcagcccgca
     7021 cggcaagtac ctgagctcgt tctccttgcc gaaccaagtt ccgacgcttc aggttgtcga
     7081 cgcgcgcacc ggtaatgtcg tccgtcttgg cggcagcagc accgccacag gctacacgga
     7141 tatcggtttc agtcctagcg acgattcact gcttgccttt tccaattcgg gcgccacact
     7201 ttatcgcttg tctgacggcg tcgctgttta caccaatacc gatccgccca gcgcctacgg
     7261 attcagcccg accggtggat atctcgagat caaggtcgtc accaacacga cacatgtcga
     7321 gctttcggtg gtcgcgaccg ttgacggcca ccccgtgcac acaaacgcgt acaacttcgc
     7381 ggtgggtgcc gccgtccagc aggaagcgtc gggcttcagc cccgacgagc acacgtttct
     7441 cgatgcctat gtcaacggca cggataccgc gtcgctcgag atcgtcaacc tgcgcgatgg
     7501 ccgccgagtc ttctcatcgt cggcaatcga ccgcggcagc acgtggtcat tcagcccctg
     7561 cggcggcgag ttcgcgatgg cggtgccaaa cggcagtcgt cagaccctca cgctgcgcgc
     7621 cacctcggac ggacgcacgc tggggtctgc gctatccgtg ccggttacgg ccctgacgat
     7681 cgaagcgact gacgacgaca acacggtcac gtatgtcgat gccaccggaa cccagacgat
     7741 cacgctggca ccaaacttgt ggtgcggcgc tgcgacgatc aatcacgaca acgacgtctc
     7801 gggcggcgac agtcgcacct ggtcgatcga actggaccgg ccggcccacg actacggcgt
     7861 cacatttcgc ttgagcagca gcgacagtca tctgaccgtg ccggcgtcag cgacgatccc
     7921 ggccggccag acgacggtgt ccttcgacgt cacctcatcg gtgccccccg aagacgtcct
     7981 cgtcaccgtc accgtggctc acgacgaggt gctcggcggc acggaggcga gcaccttcca
     8041 ggtgtcgatc cgcggtccag tgggtctgga gtctctctac ctgggcaccg gcgaaatgtc
     8101 cggccctgga acagacaccg gcagcgtata tctggtcgcg tcggctccca ccggcggcac
     8161 caccgtcagc ctggcgagcg atgatccggc tgtctccgtc cctgacaccg taatcgtggc
     8221 tgcggggacg gattccgcga cgttcccgat cacggtcgcc gcggtcgatc acaccactac
     8281 cgcaaccgtg tcggcgacat ccggcgaaac cacggtgtca gcgacgtatc tcgtcgatcc
     8341 gatcgtgacg acgagcactc gcctcgtcgt gcgacccgga acctcgaccg tcgtcggcga
     8401 acaggtgggc ctcgacgcca ccgttacccc catcgagcca ccggctgacg accaggactg
     8461 ggtctcctac tacggcacac cgaccggcac cgtcaccatc accgacgacg gcaccgagct
     8521 gacgagcatc gacgtcacgc cgcccgactt cggcggggcc gagggtcagg taaccgcatc
     8581 gtggtccatc gcggcgctcg acgccgggca gcacatcatc accgtgacct acagcggcga
     8641 cgaccactac gggccgtcac aggcatcggc gaccgtgaca gtccgtgaag ggctgagctg
     8701 gaccggaaac ggcggggacg gcaggtggtc gaatcccgcg aactggagcc ccagccgcgt
     8761 cccggatgcg caaaaccctg acatcgtcgt gatctccagt gcgacggaac tgcaccttga
     8821 cgacgacctt cccggtctcg agctgagtgg cctccatctt tcgggtccga tcacgctcga
     8881 cggccaagcg atcattctgg acggtgaact ggccgcaacg cggagcggcg ccacgatcgg
     8941 caacgacatc tcgctgacga tcgacacgca caacttctac gcggacacga tgttgcgcct
     9001 cgacggagtc gtctcgggcg cgggtgggcc gatcgcatcg acggcctcag gaggcttcgt
     9061 cgtcctggcc ggcgcggcca acacgtatac gggcgaaacg cgagtgagcg agggcgcaac
     9121 gctcatggcc ggcgccgacc aggcacttgg cgccaggggg gccagtcaag gtgccgaggt
     9181 ctatggcacc ctgtgcatct cgcgaggcgt ctctctgctc gagcctgtgg cgtcgcacgg
     9241 cggaaacgtt gcctcgtgcg gcacacccga cgacctcagc gccacgctcg ccgatgaccc
     9301 ggccgacgtc ggttcgccca ccaccgcgac gatgtctggc tcgctcctca cagacggcac
     9361 gaccgggctc ggcccgctct cctgctacgc cgacgatccg acaggtgacg agcttgacct
     9421 gaatgtcgac ggcgccatca gcggcagcgg gcaggtcagc atcggctgcc cggggcggcg
     9481 cgacgggccg tcgatgggct cggtttcgtt cggtggtgaa caacccaaca cctacacggg
     9541 tggcacgcag atcttcggca cgctgaccct cgcgaaaggc aacggcgtgc cggcagctgc
     9601 cgggaccctc gtggcggcca atggcggcac cctgaagctc gctggcgacg accagatcgg
     9661 tcactcggcg cacgcggacg tcaacattct cgatgccggc ccctatcacg ccacggtggc
     9721 cgacctggcg gtcggcgtgc tcaagacggt ggtcgacgcc gccggccaca gcggtggcgt
     9781 caccgtcctc ggcccgacac agctgaacag cgtccaggtc agtgtcgcgt ccgatcccgc
     9841 aatcggctcg acgttcacgg tggtcgacaa cacctcgtcc gatccggtcg gcggttgcac
     9901 caacggggtc gtgccgggtg atgaggcgac cagttaccgc atcgactgca caggtggtga
     9961 cggcaacgat gtggtcctga cgaccgtttg gcatccgacg gtcacgttga ccaccgaccc
    10021 gatggacggt gccgtgtcgg gttcccccgt gacagtgacc gccaccgtgt cgtcgccgtc
    10081 cggggcagcg gaggccaccg ggcaggtctc cttgaagacc ttccacttca actatgggtg
    10141 gatatatcag gccactgcgg cgacggccac tcttgtcgac ggcgtcgcaa cgttcagctt
    10201 ccccgacacc tctttcctcg cctcgggcaa tgggctggtc gcgtcctacc tcggcgattc
    10261 gcagcacgcc ccggtcaatt cgcggctgat gaggtatctg tcgtggccgg atcccacggg
    10321 cataccgctg ccagcggcac cggtgaacct gtcggccggc gtttcggggc cgggcgggtc
    10381 gagcccgaat accgcatttc tgtcgtgggg ctacccgccc ggaaccacgt acaccgatta
    10441 cgtcagctat cacatcacgg tggcgccgga ccacggtgag ccgtacgtcg tcgacaccaa
    10501 caccgcccag agcgacgacg acttgatcta ccgggatttt ccgggctttc cgcgctaccg
    10561 gctgagcggt ctgcagccgg gagagaccta cacgttcagc gtgcaagcgg tcaaccatgc
    10621 cggtgccggg ccggcctcca acgccgtgac ggtgaccgtc cccgtggctc cagcgcccgg
    10681 cgcgcccacc gacctcagcg ccatcgccgg gcctggccag gcgcagctgg cctggaccgc
    10741 gccggccgac gacggcgggg cgccgatcaa cggatatgag gtcactccgg tcaacgacgg
    10801 cgtgaggcaa gaggcgatcc ggacggccga tgccacgact catctcacag tgaccggctt
    10861 gacgcctggc gcctcgtacg tcttcaccgt cgcggccgtc catgtagggg ccgtcgggcc
    10921 gagttcctcg ccgagcgtgg cggtgacacc gaccgcgatt tccgcagccg ccgtggtgcc
    10981 cggggtgcct tccgacgtgg tggcgacgcc tgggccgggc agtttgacgc tgacgtggtc
    11041 gccgccggcg gacgacggcg gcgcgtcgat cgtgcactac gtgatcgtgc cgtcagggac
    11101 cgccggaagc aggatgcaac tggtgacgtc ggggcctgcg acgaccgtca cgctctctgg
    11161 cttgcccgac gatctgatga cctttaccgt cgcggcctcc aacgaagccg gcaccggacc
    11221 ggcgtccgcg cccaccgcgc ccgtgaggcc ggtgggggcg gtgccaggag catcgaccga
    11281 cgtactcgtg agcgctggag ccggtcaggt cttcgtcacc tggaccccgc cggccgacga
    11341 aggtggctcg ccgatagtcc gctacctagt gacgccgtac ctcgccgggg cggcccaggc
    11401 tccggtggcg acggcaggca acggcacgtc ggccacggtg gcgggtttgc taagcgacgt
    11461 gccctatacc ttcacggtga gcgcggtcaa cgccgcgggc agcgggccgg cgtcggaaca
    11521 aagtgcaccc gtgcggccac tgagcgcacc gcccatcgtc agcgtgccga ccgccccgga
    11581 aaccttcacg ggcagcgtcg acggccaagc cgtcaccttg acctgggcac caccgtcgag
    11641 caacggcggc tctgccgttg ccggctacct cgtcgaggcg ttcaagtacg cctactcaaa
    11701 ctacggcgag tggggaggcg gcacctatca gggcggtcct taccaatcgt ggtggctgcc
    11761 ggccgacgcg acatcgttca acctcacgac cgacacgatc tcctggccgt ggaccagcgg
    11821 cgcctaccgt ttcacggttt cggcggccaa tgacatcgga gtcggcgcgc aggcgtcgcc
    11881 gtggttctcc gcaccggcga tctgggtcgc accgccaact gaaccggggt cgggcggcgg
    11941 actgccgccg gagccgaccg tgccgccggg acagaatggc gacgtcagca acggtgcagg
    12001 cgcgggagtt cacggcagcg gcttcgggcc ggacgaacca gtcagtgcga cgctgcactc
    12061 gacgccggtc gacctcggca cgttgaccgc tgcggctgac ggcaccgtca cgggcacggt
    12121 gaccatcccg gccgacacac cggccggtga ccacacgatc caattagtcg ggcagaacac
    12181 cggccacgta gtcgacatcc ccatctcagt gcaccaggcg ctgagcatcg gtgacatcac
    12241 gtcgtcgtcg atgacggtgt cgtggccctc ggctccggag ggcgccacgg gcctcacggt
    12301 gagcgtcgac ggctcacccg tcaccacgct gtcgaccacg gtctcgtcgt acaccgtgac
    12361 gggcctctca ccggccacca cgcatacgtt gtcggtgcag gcgagcagcg gtgacagttc
    12421 gctcggtgag ctggcgatca ttggcacgac gttgtcgacc ctcgaggcgt cagccgcgaa
    12481 gacagcggtg tcgctgagct ggctggcgtc gtccgacccg acagtgaccg gctaccgagt
    12541 catgcgcgac gacaatgcag acggcgattt cgatgtcctg gccgatgttt cggggccgtc
    12601 gttcaccgat tttgacctac gtgcatcgac gacgtaccgg tatcacgtgc tcgccgtgca
    12661 cgccgatggt tccacctcgg tatggtccga gtccgtcgcc gcgaccacgc ccgacctcgc
    12721 catcagcaat gtgtcattct cggcgccgac cgcgttgggc ggctccaatg cggcgtacgg
    12781 ctccaccctt gacatcgagg tcacggccga cgcgggccgc acggcatcag ccgtggtatc
    12841 cggtcaggcc cccgacggtt cggcggtgtc gaagaccgtg gcgctgagcg aaacctcgtc
    12901 tggtgcctac gacggcacac tcccgctcaa cacgacgatc gccaccatcg acgcaatcac
    12961 cgcgacgttg tcggacggtg tgcacagcgt cgacgctgag gccaccgggt tgccgttggc
    13021 gcaaagcggt ttcctctcca tcgagatcag cccgtcgaac gtagacgtgg cgggggcgca
    13081 actcgagctg gtgaacaccg cgctgggcgt tgacgagatg cgggccgtcg tcgctcccgc
    13141 gcacgtcggt atcgccgtcg ccgccggttc gtggaccctg cggctgctgg ctcctgatgg
    13201 cgatgtgctc gccgagcggg ccgacgtcgc ggtcagcaat gcaaccatga gttcgctgag
    13261 cctggcaccg gtgcggcatg cgtccttgac ggtcggcctg gtggcaccgg acggtgagac
    13321 atccggcggg ttcaccgtga cggttaccga ctcggacggc gcagtgttgg gtcaaaccga
    13381 tgtcgcgtcc ggcgactcgt ccgttgagtt cgacggtctg gtcgcaggca cggcggtgac
    13441 ggtgacagca acgttggacg acgctagccg gccactgctg tcggcggtga gcacgacgtc
    13501 gacgctggcc ggcggagcca atacggtgag cctcgtcgaa caggcgctgt cgaccggacg
    13561 catccaagga ctcgtgacac cggcggccac ggtcgtggtt gaccaacaag tcgacggccg
    13621 ttcgtggcag ttcacgacga ctactgacag tgccggccat ttcggaattg acgtgctggc
    13681 ggccactgca cacgtcacgg caagcgccga cggctacctg cccgcgacct ttgacatcga
    13741 tgtcgcaccg agcacgacgg tgaccgcatc accggtcgcg cttgagccgg tggggaattc
    13801 gcgggtggcc acccggctga ccatcgatcc catcagttcg cctcgcgacc tgatgaatct
    13861 gatattcggg cgctacaaga tccaggcaca tttggtcgcc gacgacggtt cgccgatcaa
    13921 gggcgcgcgg gtcgtgatcg acagctggaa gggcccgttc tgtgtggcgg agacccacgc
    13981 gtcaggagtg gcgagctgcg acgtaccgcc gtcgcttcgc tcggtcgtgt tgctcggtca
    14041 ggtgcacgcg agctatgccg gctccgacaa gtacttgccg tcgtcgagcg gtgacatcgt
    14101 tggccccggg tggcatggga aagaccacct agccgcgtga gtcgcgggcc tgctcataga
    14161 gggccagaaa gtcctcgacc ggcaccgcgg cgatggtctc ggcgagcagg tcgaggtcga
    14221 ggtcgtcgac gcggcgaaac cgcaggcagc tcttgcccat gtcgagccga cgcccgccgg
    14281 cctgccaccg ggtgcgaaac tctcgctcgc gctcgccgtc gggatagagg gtcatgaggt
    14341 agagcgagta gtagcgcttc tgcgccgcca gggcgacgta gccgagcggc tggccgttgt
    14401 aggtctcggg gtagcgctcc agcggcacca cccagcagag cattccccag gccatcgtct
    14461 cttcgtagcc gtcgggcagg ttggccagca ccagctggcg cacctgggcc acgacctccc
    14521 ggggctcggg ggcaagaccg gcgaggtagg cctcggcggt ggtggcgtca ctgcgcatcc
    14581 gcggagtcta gacatccccg agacgccaaa gagctggatg agtgaggact cactcaggta
    14641 gcatcgcgac cgtgccttct tcgccccgac gggcagcccc gctgccgccc gatgctcgcc
    14701 gggccgcgct cgtcgcggcc acgctgccgc tgcttcgcgc ccacggcccg cacctgagca
    14761 cccgtcagat cgccgaggcg gcgggggtcg ccgaaggcac gctgttccgg gtcttcccca
    14821 gcaaggacga catcgtggcg gcggcgatcg cgcatgcgat caacccggct cggacggcgg
    14881 ccgagctggc cgccatcgac cgctcgctgc cgctgacgcc acggctggtc gcggccacgc
    14941 gggtgatcca gcgccagctc gacgggatca tcgcgctgct caccctgcgc cgtgactgcg
    15001 cgccctcgga ctccgaccac ctgcccgacc accacgccgg caccgtggtc gtgctggcgg
    15061 ggctggccca cctcgtcgag cctgaccggg acgcgcttcg ggtcgagccg gccgagctgg
    15121 cccgccggct gcgtctgctg accatcgcgg cgagccaccc tcacctcacg gacgacgacc
    15181 cgatgagcgc agaagagatc gtcgccacgc tgctcgacgg gctgcgcggg ccccgccgtt
    15241 cgcacgcgtc gaccgcccac gatgggagca ccgggtgctg actgggctgt tgcgcaccta
    15301 cctgcggccc taccgaggcc cgctggccgc ggtcgtcgcg ctgcagctgg tcggcacgat
    15361 ggcctcgctc tacctgccca gcctcaacgc cgacatcatc gacaacgggg tggccaaggg
    15421 cgacaccggc tacatcctca tgatcggtgg ctggatgctc acggtcaccc tcgtgcagat
    15481 cggctgctcg atcgcggcgg tctacctcgg cgcccagacg gcgatgagct tcggccggga
    15541 cacccgggcg gcggtcttcc accgggtcgg cacgttctcg gcccgcgagg tcaaccagtt
    15601 cggcgccccg tcgttgatca cccgcaccac caacgacgtg cagcaggtgc agatgctcgt
    15661 ggtcatgtcg gccacgatga tggtggcggc accgatcatg tgtgtcggcg gcgtcatcat
    15721 ggcgctgcgc caggacgtcg ggctgtcctg gctgatcctc gtcagcgtca gcgtgctggt
    15781 ggtgctgatc ggcgcgatcg tgtcgcggat ggtcccccag ttccgcctga tgcagacccg
    15841 catcgacgcg gtcaaccggg tgctgcgcga gcagctggcc ggcatccggg tcgtccgggc
    15901 attcgtgcgg gagcgccacg agaccacccg cttcgccggc gccaacaccg agctgaccga
    15961 caccgcgctg cgcaccggcc ggctgatggt gctgatcttc ccggcggtga tgctggtctt
    16021 caacgcgtcc agcgtcgcgg tgctgtggtt cggcgccgcc cgggtcgagc acgggcagat
    16081 gcaggtcggc gcgctggtgg ccttcctcag ctatctcatg cagatcctca tgtcggtgat
    16141 gatggcgacc ttcatggtga tcatgatccc gcgcgccgcg gtgtgcgccg gccggatcgg
    16201 tgaggtgctc gacaccgagt cgtcggtgcg cccacccgcc gctccggtca cccagctgac
    16261 ccgccagggc gagctgaagc tgcgcaacgt ggagttccgc taccccgggg cggccgagcc
    16321 ggtgctgcgt gacatcacct tcgccgccag ggcgggacag accacggcga tcatcggcag
    16381 caccggtgcc ggcaagacga cgctcgtgtc gctggtgccc cggctcttcg acgtcaccgc
    16441 cggttcggtc ctcgtcgacg gcgtcgacgt gcgcgagctc gaccctgacc tgttgtggag
    16501 ccggatcggg ctggtgccgc agcgggccta cctcttctcc ggcaccgtcg ccagcaacct
    16561 gcgcatgggc gacccgcagg ccagcgacga ggcgctgtgg gccgcgctgg aggtcgccca
    16621 ggcccgcgag ttcgtcgagg cgatgcccgg cgggctcgac gcaccgatcg cccagggcgg
    16681 caccaacgtc tccggtgggc agcgtcagcg gctctcgatc gcccgggcgc tggtccgccg
    16741 gccggagatc tacctgttcg acgacgcgtt ctccgcgctc gacctggcca ccgacgcccg
    16801 gctgcgggcg gcgctgcaac cgctcaccgc cgacgccgtg gtcctcatcg tggcccagcg
    16861 ggtctccacc atccgcgacg ccgaccagat cgtcgtgctc gaggacggcg cggtggtcgg
    16921 cctgggcact cacgacgagc tgctcaccag ctgtccgacg tacgtcgaga tcgtggcctc
    16981 ccagcacgcc gcggaggagg ccgcatgagc gcgaccaccc gggtgccccg gcgcccgcat
    17041 cccggccacg gcccgtttgc cgcggccggg gcgcccaccg acaaggcgat gacgttcggc
    17101 ccgtcggcgc gccggatgct cgcccggctg cgcccgcacc ggaccagcgt ggtcgcggtc
    17161 ctcgcgctgg cggtggccag cgtcgcgctc agcgtggtcg ggcccaagat cctcggccgg
    17221 gccaccgacc tgatcttcgg cgggctcatc ggcgcccagc tgcccgcggg caccacccgc
    17281 gaccaagccg tggcggcggc ccgcgacgcc ggcaacacca acttcgccga catgctcgcg
    17341 cgcatggccg tcacgcccgg ccagggcatc gacttcaccg cgttgagcca ggtgctctcg
    17401 ttcgtgctgg tgctctacct ggccgcgtcg gtgctcggct ggctgcaggg ctacctgctc
    17461 aacgacgtgg tgcaggccac ggtgttccgg ctgcgagccg acgtcgagga caagctgcac
    17521 cggctgccgc tgcgctacgt cgaccagcag ccgcgcggcg agctgctcag ccgggtgacc
    17581 aacgacatcg acaacatcca gaccacgctg cagcagaccc tgagccagct gctcacctcg
    17641 ctgctgaccg tgctcggcgt gctggtgatg atgttcgtca tctccccgct gctcgcgctg
    17701 atcgcgctgg tgaccatccc gctgtcgatg gtggtcaccg cgcgcatcgc ccagcgctcg
    17761 cagccgctgt tcatcgccca gtggacccac accggcgcgc tcaacgcgca gatcgaggag
    17821 gccttcaccg gccacgagct ggtcaaggtc ttcggtcgcc agcgcgaggt cgaggccgcc
    17881 ttccacgcca agaacgacga gctgttccgg gccagcttcg gcgcccagtt cgtgtccggg
    17941 atcatcatgc cgacgatgat gttcatcggg aacctcacct acgtcgccat cgcggtggtc
    18001 ggagggttcc gcgtggccac cggcgcgatc agcctgggca acgtgcaggc gttcatccag
    18061 tactcccgcc agttcacgca accgctgacc caggtggcct cgatggccaa cctgctgcag
    18121 tcgggggtgg cctcggccga gcgggtcttc gagctgctcg acgcgccgga gcaggcgccc
    18181 gacccggccg cgccagcccg gccggagcgg ttgcgcgggc gagtcgagct cgagcacatc
    18241 acgttccgct acgagccgga ccagccgctg atcgacgacc tgtcgctggt cgccgagccg
    18301 ggccacacgg tggccatcgt cggacccacc ggggcgggca agaccacgct ggtcaacctc
    18361 gtcatgcgct tctacgagct cgacggcggc cggatcaccc tcgacggcac cgacatcacc
    18421 acgatgcgac gcgacgagct gcgctcctcg atcggcatgg tgctgcagga cacctggctc
    18481 ttcggcggca cgatccgcga caacatcgcc tacggccggc cgggcgcttc cgaggacgag
    18541 atcgtggccg ccgcaagggc ggcgtacgtc gaccggttcg tgcgcgccct gccggagggc
    18601 tacgacaccg tcatcgacga ggagggcacc aacgtcagcg ccggcgagaa gcagctgatc
    18661 accatcgccc gggccttcct cgccgacccc gcgctgctga tcctcgacga ggccaccagc
    18721 tcggtggaca cccgcaccga ggtgctggtg cagcacgcca tggccgcgtt gcgcgccgac
    18781 cgaaccagct tcgtgatcgc ccaccggctc tcgacgatcc gcgacgccga caccatcctc
    18841 gtgatggagg ccggccggat cgtcgagcag ggcacccacg agcagctgct ggccgcgggt
    18901 ggggcctacg cgcgcctcta ctccgcccag ttcagcggcg cgctcgccga gacctcgtag
    18961 tggcgcacgg tccactcccg ggccagcaag aagtcgtcgt cgcgggcgta gaacaccgcc
    19021 cggtccgggg tgccacccgc gaacgccgtg accgcctcga tcgactccca catgctcagg
    19081 gcgcagaact ccacccgctc gccgtccgaa cggcgcagca tccacgcccc acggttgccg
    19141 gccgcgcccg cgtaggcagg gatggcgacc tcggccatgt aggccgcgta gtcatcggcc
    19201 cgttcgggcg caacccagcc gcgccactgc cgaacgatca cgacgcggcc ggttcaggag
    19261 gccggcaggg ccagcaccga cagcaccgcg ccctgcgggt cggcgatcat cgcccagcgc
    19321 ccgtactcgg tgtcgcgcgg ctcggccagc acggtcccgt ccatctcgcg cacggtggcg
    19381 accgccgcgt cggcatcggt caccgcgaag taggtcatcc agtaggacgg cgcctcgggc
    19441 ggcacgtccg tcggcatctg catcatcccg gccacctggg tctcgccgat ggagaacacc
    19501 gtgtagggct cggtcatgcc gatgtcctgc gtctcgcggt cgaacccgaa caccgccgcg
    19561 tagaactcgc cggtggcctg cgggtctcgg ctgagcagct cggtccaggc caccgacccc
    19621 ggctcgccga gcagcccaga tcccggcatg tcgcgcggct gccagacgcc gaacacggca
    19681 ccagccgggt cgcgacccac cagcatccgg ccctgggtca tcacgtccat cgggcccatg
    19741 agcaccgcac cgtcgtgctc ggtcacccac ttgcccacgt cgtcggcgtt gtcgctggcg
    19801 aagtaggtcg tccagctcgg cgggaccgcg gtctcgagca tctccgggga catcgccatc
    19861 atcccggcga tcggccggtc cttgagcatc gcgagctggt agtgcccgcc ctcctccccg
    19921 gtgtcgacgt agctccaccc caacaggcgg gtgtagaact cctgcgccgc cgtcaggtcg
    19981 gtcgtgccga ggtcgaccca gcatggtgca ccgctgggat agttgcgcat ctcggtcatc
    20041 agggccctcc cgtggcttcc cgtcgtccac gccgtacggc gcagccgctg tccacactga
    20101 cccctgctcc gccggccgtc tagggcccgc acgggccgac cgcgccgtcc acaaccgggc
    20161 cgctcgccct ggtgaggtgc ttggccggct gcgacaatcc ctcgcgtgtc gggaagtttc
    20221 gcgggttggc tgcggggcct cgacgaggcc cggttggtgc gcctgctcga gctgcgcccc
    20281 gactcgtgcg gcccgccggc acccgagagc atggaggccc tcgccgagcg gctgtcgtcc
    20341 atgcagtcgg tgcaccgagc actgggtgag ctctccgagc tcgccctggt ggtgatcaag
    20401 gtggtgcagg ggctgggcgg gtcgtgctcg cgcgccgaga tcgacgagca tctgcacctg
    20461 ccctcgccgg ccgaccaggc cgccgtcgag ctcgcggtgg ccgccctcgt ggctcgcggc
    20521 ctgctgctcg accgcgacga ggcgttgccc cgccagggcg ggggcccaga cgggctcgaa
    20581 ggtggtctga ccctggtcaa gaccgtccgg gcgctgttcc caggcccctt cggcctgggc
    20641 cggccgctgc gccagctgct cggggccacc tcgcccagct ggctcgccca gatgcgcacg
    20701 acctaccggc tgccggaggc cggtcgtggt gagacccaca ccgcgctcgt ggccttgctg
    20761 acgcggcggc gaatcctcga cgaggtgcgt cacggcccgc cccggctgac cgagctgctc
    20821 catgacgtga tggccgacgg cgggatcgtc gtactcgagg gcagcgggac cttctccctc
    20881 gacgtcagcc agcgcaccgc gggcaccgac gaccggctga cctatgccgt cgaccatgcc
    20941 ctgctcgtgc ccgtcgactg gcaggtcctg gagctgccca acgaggtggc gctggccctg
    21001 cgctcccccg gctgggcgct ctcggcgccc acctcgccgc ctgagccgcc caccgcggcg
    21061 accgatcacg cgacggtcga gcgcgagagc ggcgcctcgg gcgccgccgc ggtcatcgtg
    21121 gtgaccgacc tgctcgagct gctctcggcg acgccggcgc gcgagctgaa gaccggcggg
    21181 gtcggcatcc gcgaggtccg ccggctggcc aaggccctcg gccgctccga ggccgacgtc
    21241 gcgatggctc tgcagctggt gatcgccgcc cggctggtgg gggtcacggg cctggaggtg
    21301 gtcccgcgcg cggagtacga cgagtggcgg atgcgcaccc ccgccgaacg gctcgcctca
    21361 ctgctgtcgg cctggtggtc tcggcccggc tgggtcagcg gcgagctcga cgacgacgac
    21421 aaaccgctgc cgcccgtgtc gccacgtcgt gccgatgaac gagtcctggc gctgcggcag
    21481 gcgatggtgg gcgtcctcgc cgcgctgccg gccgggcggg gcgcggtcga cgccgccggg
    21541 gcagccgcca ccgtcgcctg gtcgcgcccg ctcgggttcc cgcaggggca gcagctgacc
    21601 gcggccgccc aggcgacctg gtgggagggg cactggctgg gcgcgctggc gcacggccgg
    21661 gccagcgccg tcggggcggc tctcacgtcg tacgacgagg atcggcttct gcaggcctgc
    21721 caggtgctgg tgcccgaggc gatcagccaa gcgatgttcc aggccgacct gacggccgtc
    21781 gtggccggca gcccgagcag cgaggtcgcc tccttgctgg actcgtgcgc cgaccgggag
    21841 gccagtggcg gcgcggtgat ctggcggttc tcacccgcca ccgtgcgccg ctgcctcgac
    21901 accggccagt cagccccggc gctgctgcgg gcgctcgggg ccatcgcgcg agacaccctg
    21961 ccgcagccgt tgacctacct ggtcaacgac atggcacgcc gacacggccg catcgcggta
    22021 cgcccgctgc gctgcgtgct cgtttcccag gacgccgcgc tgctcgctga gctggctgcg
    22081 caccgcgcgc tgcgcccgct cgcactgtcg gtggtggcgc ccaccgtgct ggccagcgcg
    22141 aagtcgctcg acgagacggt caccctgctg cgtcaggccg gctacgcccc ggtgcggcac
    22201 acccagaccg gcgagctcgt cgtggacagg gccacccctc ggcgggtcaa ggtcaccccc
    22261 cggccgaccg ccaccactcg cgcaccccga ccaggcgagg cggcgcccac cgagccggtg
    22321 acgccggccg cgctggcgaa ggcgctgcgc gaggcgtcca ggcaccccgg cactcccgcg
    22381 ccgggcaagg cgcgaccagc tccgcccgcg ccgccccggc ccgcctcctc agctgcgagc
    22441 accctggcga ggctggcaga gcgtgccggt catctctccg acgacgagcg gagcctgctg
    22501 gcccatgcga tcgacttcgg gggcaaggtc ggcatcgact accgcgacgt gcacggcagc
    22561 ttcagcaccc gtgccatcag cgacgtcgct ctcgacccgc cctatctcga ggccttctgc
    22621 cacctgcgcg aggacgaccg ggttttcctc ctgtcgggca tcagcgcggt ctatcccgcc
    22681 tgagccgacc cggtggtaga acacactgca tgcttgagcg acggttgccc cgctgggccg
    22741 aggtgcgcga gctgctgcat ccgcacgccc ccacgtttga caacacagcc cgccggctcg
    22801 cgaccgtcgc ctcgatcagc gacctgcgcc ggctcgcccg acggcgggcc ccgcgcgcgg
    22861 tgttcgacta caccgacggc gcggccggcg acgaggccag cctgcgtcgc tcgcgggcgg
    22921 cctttgccag cgtcgagttc tgcccctcgg tgctgcacga cgtgtccacc gtcgacacca
    22981 gcacgaccat cctcggggca cccagcgcgc tgcccctggt gtttgccccg accgggttca
    23041 cccggatgat gcacgccgag ggcgagcctg ccgtggcccg ggtggccggc cggaccggga
    23101 tcccctacgc cctctcgacc ctcgggacca cgtcgatcga gcggctcgcg gccgacgcgc
    23161 cggacacccg acgctggttc cagctctacc tgatgcgcga ccgcggagcc ggccgcgact
    23221 tcgtcgctcg cgcccaggcg gccggctacg aggcgctcgt gctcaccgtg gacacccccg
    23281 tcgccggccc gcgacgtcgc gacgtacgca atgggctgac gatcccgccc gcgcccacgc
    23341 tgcgcaccgt cgccgaaggc gcgctgcacc cgggatggtg gtacgaccta ttgaccaccg
    23401 agccgctgga gttcgcctcg ctcaaccggt tcgagggcac ggtcgccgag ctcatcgcca
    23461 agacgttcga cgcctcggtg acgatggccg acctcacctg gctgcgcgag acgtggaagg
    23521 gaccgctcat cgtcaagggc gtgaccagtc ccgccgacgc caaggcggtc gtcgaccacg
    23581 gcgccgacgc cgtggtcgtc tccaaccacg gcggccgcca gctcgaccgt tcgccgacgc
    23641 cgttcgaggt gctgccggag gtcgtcgcag tcgtcggcga ccgggccgag gtctacgtcg
    23701 acggcgggat cctctcgggc gccgacgtcg tcgccgcggt ggcacgcggc gccaaggcgg
    23761 cccttgtcgg ccgggcctac ctctatgggc tgatggccgc cggtgagcgc ggcgtggaga
    23821 aggctgccga catcctcgcc accgagatcg ccaacacgat ggcgctgctg ggcgtcacct
    23881 cggtcggcga gctcggtcct gagcacgtgc ggatccgccc cgcctgacta gcgcgctgat
    23941 acgacctcaa ggtcggtgaa ggcggcgatc gcgtcgaagt cgatgtcctt gtgcagcagc
    24001 gccacgccat gacgcatggc gattgctgca atcaagcagt caatgagacg gcgaaccgtc
    24061 acgccacgcc ggcgcgcggc tcggtaaagg gtggctgctg ccgtgaagtc gagggcgggt
    24121 tggaccgaca gcatggtcag tccgccggta agccgctcaa gccggtcaac agccacgtcg
    24181 tcggcggcac cggcgagaag ctccatgacc accggctcgg tggttgccag tgcccgtcca
    24241 tcggtgatcg cctctcgtag ggcggcgctg gccacgctgt cccgacccct gaggtagtcc
    24301 acccacaccg aggtatcaac gaggagcacg cgttactcgc cggccgcttc gtcggacgca
    24361 cgtgacgagc gcatcgattc caggtcgcct tcccagcccg agccctcaag gtcacgcaga
    24421 aactccgggg tcaacggggg accgaccagg cggcgcagcg cgagatcgac ggcggcgcgc
    24481 ttgctcgcga gcccgaagcg tcgcatgacg gcggcgacca gctcgtcatc gatctcaatg
    24541 ttggtgcgtc ccatacacca atggtacaca tcagcgcgcc ttccgacgcc ggcgtgccgc
    24601 tctggttcga cgagcctggc gcagcggtgg ctgccgcgag cgcgacccac ggcgtcgagc
    24661 cagctcgcgc tcgaacgacg gcgtctcgcc acgcgcgatg gcctccatgt cgcggcgcac
    24721 gttgcccagc agcgcccgca cctcggctgg tggcatctgc ccctgcagcc cgcgcaggag
    24781 caggcgggcc atctcgtagt cgggctcggc ggccagcgcc cggtcgaccg cgacgttggc
    24841 cagcgcccca tcaccgtggg cgtaggcgac ccaggccagc actgtgcaga cgggggcggc
    24901 ccactcgtca tcggtgcgct ggcacaactc ggtcaggaaa gacaggtagg cgtcgtcatc
    24961 cagatcgagt gccagggtgg cggcgtagtc gcggcaccac ttgctgctca agccgagcac
    25021 gatccgcgcc gcctcaaccg gcgccaggtc gcgctcccct gcggcccagc gcgaggccgc
    25081 ggactcaagc agcgccatcg tctcggcggt gagggcatcg gcgccgtcct cggccgccgc
    25141 gatgaccgcc tgggcggcct gatcaaaggc cagcatgcgc gccccgtcac tcaacggccc
    25201 ccgacgcgga cggatgctgg cctcgagggc ggcgcggtcg gccagcaccg cgcggccgtc
    25261 gaaggcggcc gcagccgaga cgatggctgc cggcccttgt ggttcggacg ggatcggagt
    25321 cccttcgggc gggcagcacg ccaacgcgca cgagtaggac caccaccggc cctgccgcac
    25381 gagcagacac tcgacgacct cgatgcccag ccgatcgacc agatcgtcga cgaattcgtg
    25441 ctggggcaag gatccatcga catcgggggc gtcggtgtag acgaccagga tgacgccgtc
    25501 ggcctccgcc cgccgtacgt gccaggccag gacgtcggcc agctcctcaa gctgttcgat
    25561 cggcacgagg tcgtggcgca tgaccagacc ggcgcgccgg cgcggcccgt gcaggcacac
    25621 gacgacgatg ctctcggtcg gcacgaagcc aacgcgcagc ggcacgacgc cgacgatgtc
    25681 ggtggggctg ctgaacttca cgacggggcg cggttgtgac gacatggccg cgagcctggc
    25741 cgggccgctc acgtcgtacc aggccgtgat tgctgcctgt ggacaactgc ggccggtcgc
    25801 gggtctgtga gtcgccgcga tcacctgcgg gtcgcgaccg ggcaaaccta gggtgcccgg
    25861 catggacctc gtggagctca cgcccagccg cgagcagtac gcctacacgt tcggtggggt
    25921 cgcgccgctg atgacgatca agccgggtac ggcgctgcgg ctgtggtccg acgaggcgtt
    25981 cggcggggtg ctgcgcagcg tcgacgacct gtccagcgcg cgggtggacc tgcgcttcgt
    26041 caacccgcag accggtccgt tctgggtcga cggcgcgcag ccgggcgaca cgctggccct
    26101 gcatttcgtc gcactggagc cggcccggga ctggggcgcc tcggcggcga tcccgttctt
    26161 cggcggcatg accagcaccg accgggtggt gacgctgcag gacccgttgc ctgacaccac
    26221 ctggatctat gagctcgacc gggcccgcaa cacggtgacc ttcgcggccc ggcacagcga
    26281 gcaccgcatc gagctgcccg tcgagccgat gctcggcacc gtcggggtcg ctcccgcagg
    26341 tggtgaggcg cgcagctcgc tggtgcccga ccgcttcggc ggcaacatgg acaccccgca
    26401 gatgcgcgcg ggatccaccg tcttcctggg ggtcaacgtc gagggcgcac tgttctcgat
    26461 cggcgacggg cactaccggc agggcgaggg cgaggcctgc gggacggccg tcgagggtgc
    26521 gatgaccacc accctgatcg tcgagctgat caagggcggc gcgccgggct ggccgcggat
    26581 cgaggacgac acccactgga tgacggtcgg gtcgagccgc ccgctcgaag acgcgtggcg
    26641 catcgggaat gcggagctcg tgcactgggt ggccgagctc tatggcctgc acccgatgga
    26701 cgcctaccag ctgtgctcgc aggtcgccga ggtgccgatc gccaacgtcg tcgatgccaa
    26761 ctacagcgtc gtcgtcaagg ccgccaaggc gctggtgccc gcggcgacgg catttgacgg
    26821 cctgcacgcc gagctgcggg agcgggccgc ctcgctcggg tcggtgtgag ccggcatccg
    26881 tcgtacaggt gctgagaagg gggatggcat ggacctgcag ctgggcggca aacgtgtcct
    26941 cgtcaccgga gggacccggg gcatcggccg ggcgatcgtc gaggcgttcc tcgacgaggg
    27001 tgcggtggtc gggttctgcg cccgcaagcc cgacgaggtc gccgccacgg agtccgcgct
    27061 cacctcgcgc ggcacggttc gcggcacggt gctcgacgtc ggtgaaggtc ccgcgctggc
    27121 tgcctgggtg tcggaaacgg ccgaggcgtt cggcgggctc gacgtcgtgg tggccaacgt
    27181 cagcgcgctg gccatccccg acaccgacga gaactgggag accagcctca acgtcgacct
    27241 catgcacacg gtgcgactgg tcaaggcggc gatgccccac ctcgaacgca gcgacgccgc
    27301 ctcgatcgtc gcgatctcca gcgtgtctgg gcgggagtca gacttcgcct ccgggcctta
    27361 tggcaccgcc aagacggcga tcgtcggcta catccacggg ttggcgctgc agctggccga
    27421 gaagggcatc cgggccaact gcgtctcgcc gggcaacacc tacttcgagg gcggggtgtg
    27481 ggccagcatc gaggccggcg atccggcgct ctacgagatg gcggtcgggc tcaacccgac
    27541 cgggcacatg gggaccccgg ccgaggccgc tgcgccggtg gtcttcatcg ccagcccgcg
    27601 agccagccgg atcagcggcg ccaacctcgt cgccgacggc gcgctcacgc gcggcatcca
    27661 gctctgaccg gacccgggtg gcaagctagg actcgtgatc atctccgggg ccgaggaggc
    27721 gctgcgccag cgcatcatgg cgagcctcgc ggagcggacg gcgcacaacg gtggtgtgat
    27781 cagccgagcc gagctcagcg acttctcttt cgcgccggga gaacggcgtc gtctcatcga
    27841 cacctcccgc ggcatctgga acccgcggga cctggcggcc acactcacga tcgtcagctc
    27901 ccccaacggc ccgtacgacg accaggaggt cgagggcggg ctgttccgct acgactaccg
    27961 cgccgggtcc gaccacggcg acaacaccaa gctgcgccgc gccgccgagc tcgggctgcc
    28021 gctgatcctt ctgcgcaaga tcgcccccgg cgtctacgtg ccgatctttc cggtctacgt
    28081 ggtctccgac gacccgtcgc gcaaacagtt cctcatcgcc ctcgacgaga gcctgcgctt
    28141 cctgcgcgat ccgctgcacc cgacggccgc cgagcgacgc tacgccgagc gagtcgtgcg
    28201 ccagcgcctg caccagccgg agttccgcgg cagggtgatt cgggcctatg aaacccagtg
    28261 cgccatctgc cggctgcgcc acggcgagct gctcgatgcc gcacacatcc ttgccgacgc
    28321 cgatggcggg ttaccggtgg tcaccaacgg cctggcgatg tgcaagatcc atcacgcggc
    28381 ctatgaccgg gacttcttgt cggtcgatcg ggactacacc atccacatca agccgtcgct
    28441 tctcgaggag cgcgacgggc cgatgctgct gcacggtctg caggaggtgc acgggcacac
    28501 gatcgaagtg ccgcacgcca aggccgagcg cccggacccg acgctgttgg acgcacgcca
    28561 tcgtgtcttc ctggcttcag cctgaacggc ccacttatct gcgtctgatg gtttcgaggg
    28621 tgtagcggtg gtgctggcgc cattgctggg cgcggtcgag aactctcggt ggctgccatt
    28681 cgacctgccc gttggccgag gacaggcgtg atcgccagcc ttggcggtgg gcctggtcgt
    28741 ggtggtagtc gcagagcagg gcgccgttgc acaggtcggt ggggccgccg tgctcccagt
    28801 ggtcttggtg gtgggcctcg caggcgcggg cggggtggtg gcagccgggg aaggtgcagc
    28861 cgccgtcccg cagctcgagc gcccgacgct ggctggtgct gaacaggcgt cgggctcggc
    28921 caaggtcgag caccgtggat gcgccgccca tgacggcggg cagcaggtcg gccccgcagg
    28981 cgagccgacg cagctcgtgg gcggggatct tgaccccggt gtcgagcacc ccgttggtca
    29041 ggcggcggtg gagcaggtcg tagtcgcagg t
//
LOCUS       DATIBB010000170         4584 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_1813865, whole genome shotgun sequence.
ACCESSION   DATIBB010000170 DATIBB010000000
VERSION     DATIBB010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 4584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4584
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1813865"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(25..2109)
                     /locus_tag="VLC50_06635"
     CDS             complement(25..2109)
                     /locus_tag="VLC50_06635"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HSF27181.1"
                     /translation="MPSSLESLLRPETIAVLGASRDPHKWGQRVIRYTLAAGFPGEIL
                     AVNPNAAPGDIPGATVVASLEQAEAPVSCAFLAVPRDRVLAEVERCCALRVPTVVVAA
                     SGFGESSAEGAEIERRMVALARASGTRLVGPNCFGVFSAQRGVNLTPFEYPPHGRVAM
                     VSQSGNLAAELFLEAARARIGFSHCIGIGNQIDVGFGDLLTAFADDPGTEAVALYVEG
                     LQTGAGPAFVAGLHACRAAGKPVVVIKAGASRLAAAVAQTHTRSLAADDRVWETVLEQ
                     CEAIRVWSVPEMIDVLRCAVSLRSIGPRVAVVTDGGGDSVLALDGLSRTRLQPAVLTP
                     ATIATLEVMIPPAAPRSPGMNPLTLDTAGGVEDDPQILARCAQVVAEDPGTDVVILSG
                     LFGTYQDARDEEIKAALALRDLAAAGGAALVFHAPLPPAESEPLALLADAGVPIFDSL
                     DRAMRAVDRLVPAPEPGTRPGLSSPAGEALGGPRPWPVLEAAARLRRFGVAVPRIEVV
                     SDRAALFAAVDELGYPVCLKTADPDVVHKSDVGGVHVNLETPVQVGRAAEQLWVGDPA
                     SPVLVMPSFPRGFELLVGGFTDPCFGPVVMIGRGGVLAEVEADTALVSGDISCEAVLT
                     ALTRLRCYPVLRGYRGSPALAVDAVAELVVALGGALRDDPSIAVDLNPVLLYADRCAI
                     ADVRVMSEKKSA"
     gene            complement(2146..2598)
                     /locus_tag="VLC50_06640"
     CDS             complement(2146..2598)
                     /locus_tag="VLC50_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020543202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HSF27182.1"
                     /translation="MRRFDTPHQLLDVVGATLGTSTAHIIGQERVDQFAAATGDRQWI
                     HVDVERAKTGPFGSTVAHGYLTLALVPVILDEVFQVDGAVMILNYGLNRVRFPAAVPV
                     GSELRGTVEVRSAALHGADVQAELRVTIQVPGAERPCCVAEPVLRYVT"
     gene            complement(2601..3785)
                     /locus_tag="VLC50_06645"
     CDS             complement(2601..3785)
                     /locus_tag="VLC50_06645"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013130694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HSF27183.1"
                     /translation="MQDPVVVVEGARTAIGTYGGSFKDTPTFELGAAAIRAALERAEV
                     SPEEVDEVVLGCVGQVGPEAFNARRAALAADLPVTSNAYNVNRLCGSGLQAIWSAAME
                     LTSGEATIAVAGGNENMSLQPFLDYRARSGYQLGDRTVLDGTLSLVTDPWGRYSMGVT
                     AERVAEKFGVSRQQQDEFAALSQERAGKALASGLFDPEIVPVEVKERRTMRAVTVDEH
                     PRPDTTVEKLASLRTVFQKDGTVTAGNSSGINDGAAALVLMRESVARSRGRKPLGRLL
                     AMAKGGTAPEIMGYAPTVAIARVLEKTKLSVDDIDVVELNEAFAAQAVAVIRDAGLDP
                     ERTNPNGGAIALGHPVGATGAILTLKLLHELRRREAELGMVTMCIGGGQALAAVFQVP
                     GD"
     gene            complement(3823..4554)
                     /gene="fabG"
                     /locus_tag="VLC50_06650"
     CDS             complement(3823..4554)
                     /gene="fabG"
                     /locus_tag="VLC50_06650"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013243591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HSF27184.1"
                     /translation="MSRLRDRIAVVTGAGRGIGEGIARRLAADEALVVCADVVAENAV
                     KVAAECGGSSYGMALDVSDSAACDALIAQVVETHGRLDILVNNAGINRDAMLHKMTDE
                     AWHQVIAVDLSGEFFLGRAAARVMRAQKSGRIINISSASWMGNIGQANYAAAKAGVVG
                     LTRTMAKELARSGVTVNALCPGFIETEMTRGIPPEIYEAQLKKIPLGRVGTPTDVGNL
                     VAFLASDDASYITGEVINIGGGYQI"
BASE COUNT          714 a         1641 c         1572 g          657 t
ORIGIN      
        1 gatcgatcga tcggtgtcaa gagcttacgc tgatttcttt tccgacatca cgcgtacgtc
       61 ggcgatggcg caccggtcgg cgtagaggag cacggggttg aggtcgacag cgatgctcgg
      121 gtcatcacgc agcgcgccgc cgagcgccac cacgagctcg gccacggcgt ccacggccag
      181 cgcggggctg ccccggtagc cgcgcagcac gggatagcac cgcaatcgcg tgagcgcggt
      241 caggacggcc tcgcaggaga tgtccccgct gaccagtgcg gtgtcggcct cgacctcggc
      301 gagcaccccg ccgcgcccga tcatcacgac cggcccgaag cacggatcgg tgaacccgcc
      361 caccagcagc tcgaagccgc ggggaaagct cggcatgacg agcactgggc tggccgggtc
      421 accgacccac agctgctcgg ccgcccggcc gacctgcacg ggcgtctcga gattgacgtg
      481 cacccccccg acgtcggact tgtgcacgac atcgggatcg gcggtcttga ggcacaccgg
      541 atagccgagc tcgtcgacgg cggcgaacag tgcggcccgg tcggagacga cctcgatccg
      601 cggcacagcg acgccgaagc gccgcagccg ggcagccgcc tcgagcaccg gccacgggcg
      661 cggaccccca agggcctcac cggccggaga cgaaagacca ggccgagtcc ccggctctgg
      721 agccggcacc agccggtcca cggcccgcat ggcccggtcg agcgagtcga agatcggcac
      781 accggcgtcg gcgagcagcg ccaggggttc ggactcggcc ggcggtaacg gcgcgtgaaa
      841 caccagggca gccccgccgg cggccgccag gtcgcgcaac gccagagccg ccttgatctc
      901 ctcgtcccgg gcatcctggt aggtcccgaa caggccgctg aggatgacca cgtcggtgcc
      961 cgggtcctcg gcgaccacct gggcgcagcg ggccaggatc tgcgggtcgt cctcgacgcc
     1021 accggcggtg tcgagggtga gtgggttcat gcccggcgac cgcggcgctg ccggcgggat
     1081 catcacctcg agagtcgcga tggtggccgg ggtcagcacc gcgggctgca gccgggtgcg
     1141 ggagagtccg tcgagcgcga gcacagagtc gccaccacca tcagtgacca ccgcgacccg
     1201 cgggccgatc gagcgcagcg agacggcgca ccggaggaca tcaatcatct cgggcaccga
     1261 ccagacccga atcgcctcgc actgctccag cacggtttcc catacccggt cgtccgctgc
     1321 cagggaccgg gtgtgcgtct gggcgacggc agccgccagc cgggaggcac cggccttgat
     1381 caccaccacg ggtttgccgg ccgcccggca ggcgtgcagg ccggccacga acgctggtcc
     1441 ggcgccggtc tgcagcccct cgacatagag ggccacggcc tcggtgccgg ggtcgtcggc
     1501 gaacgcggtc agcaggtcac cgaagccgac gtcgatctgg ttgccgatgc cgatgcagtg
     1561 cgagaagccg atgcgtgctc tcgccgcctc gaggaacagc tcggcagcaa ggttgccgga
     1621 ctggctcacc atcgccaccc ggccgtgcgg cgggtattcg aagggggtga ggttgacccc
     1681 tcgctgcgcc gagaagaccc cgaagcagtt gggaccgacc agccgcgtgc cggaggctcg
     1741 agccagcgcg accatgcgcc gctcgatctc ggctccctcc gccgagctct cgccgaagcc
     1801 agacgccgcg accaccacgg tcggcacacg cagcgcgcag cagcgctcca cctcggcgag
     1861 cacccgatct cggggtacgg cgaggaatgc gcacgacaca ggggcttcgg cctgctcgag
     1921 gctggccacc accgtcgcgc ccgggatgtc gcctggagcc gcgttggggt tgaccgcgag
     1981 gatctcgccc ggaaagcccg cggcaagggt gtagcgaatg acccgctgcc cccacttgtg
     2041 cgggtcacgg gaggccccga gcaccgcgat ggtctcggga cgcagcaggg actcgagcga
     2101 gctgggcact cgtcgtacct ctctggttga tcgggcgcag acgcgtcagg tcacgtagcg
     2161 caggaccggc tcggcgacgc agcagggccg ttcggccccg gggacctgga tggtgacacg
     2221 cagctcggcc tggacatcgg cgccgtgaag cgctgccgag cggacctcga ccgtgccccg
     2281 cagctcgctg ccgacaggta ccgcagccgg gaaccggact cggttgagcc cgtagttgag
     2341 gatcatcacc gcaccgtcga cctggaagac ctcgtcgagg atgaccggga ccagcgccag
     2401 ggtgagatag ccgtgagcga cggtcgagcc gaacggcccg gtcttggccc gttcgacgtc
     2461 gacatggatc cactggcggt cgccggtggc cgcggcgaac tggtcgacgc gttcttggcc
     2521 gatgatgtgg gcggtgctcg tgcccagcgt ggcccccacg acgtcgagca gctgatgagg
     2581 agtgtcgaag cggcgcacgg tcagtccccc ggcacctgga agacggcggc cagcgcctgg
     2641 ccgccgccga tgcacatggt gaccatgccc agctcggcct cacgccgacg cagctcgtgc
     2701 agcagcttga gcgtgaggat cgctcccgtc gcgcccaccg ggtggcccag ggcgatcgca
     2761 ccgccgttgg ggttggtgcg ctcggggtcg agcccggcat cacgaatcac cgccaccgcc
     2821 tgcgcggcga aggcctcgtt gagctcgacc acgtcgatgt cgtccaccga cagcttggtc
     2881 ttctccagca cccgcgcaat ggccaccgtg ggcgcatagc ccatgatctc tggcgcggtg
     2941 ccgcccttgg ccatcgccag caaccggcct agtggcttgc ggccgcggga ccgagccacc
     3001 gactcgcgca tcagcaccag ggccgcggca ccatcgttga tacccgagga gtttcccgcc
     3061 gtgaccgtcc cgtccttctg gaacacggtg cgcaggctgg ccagcttttc cacggtggtg
     3121 tccgggcgcg ggtgctcgtc aacggtgacg gcccgcatcg tgcgtcgctc cttgacctcg
     3181 accggcacga tctcgggatc gaacaggccc gatgcgagcg cctttccggc gcgttcctgg
     3241 ctcagcgcgg cgaactcgtc ctgctgctgg cgcgagacgc caaacttctc ggccacccgc
     3301 tccgcggtga cccccatcga gtagcggccc cacgggtcgg tcaccagcga gagggtgccg
     3361 tcgagcacgg tgcggtcacc gagctggtac cccgaccgcg cccggtagtc gaggaacggc
     3421 tgcaggctca tgttctcgtt gccgcccgcc acggcgatgg tcgcctcgcc gctggtcagc
     3481 tccatcgccg cggaccagat tgcctgcagc cctgagccgc acagccggtt gacgttgtag
     3541 gcgttgctgg tcaccggcag gtccgcggcg agcgcagcgc ggcgggcgtt gaaagcctcc
     3601 ggcccgacct ggccgacgca gccgagcacc acctcgtcaa cctcctccgg cgacacctcg
     3661 gcccgctcga gtgcggcgcg gatcgcggcg gctccgagct cgaaggtggg ggtgtctttg
     3721 aacgacccgc cgtaagtgcc gatcgcggtg cgtgcaccct cgacgacgac aacagggtcc
     3781 tgcattggct ctctcctcga cgctgtgcgg cgggctgcgc ggctagatct ggtagccgcc
     3841 accaatgttg atcacttcac cggtgatata gctcgcgtcg tcagaggcca agaacgccac
     3901 caggttgccg acgtctgtgg gggtgccgac gcgcccgagc gggatcttct tcagctgcgc
     3961 ctcgtagatc tccggcggga tcccgcgagt catctcggtc tcgatgaacc ccgggcacag
     4021 cgcattgacc gtcacgccgc tgcgggccag ctctttggcc atggtgcggg tcagcccgac
     4081 cacaccggcc tttgccgcgg cgtagttggc ctggccgatg ttgcccatcc agctcgccga
     4141 ggagatgttg atgatccgcc cgctcttctg cgcgcgcatc acccgagcgg cggctcgccc
     4201 gaggaagaac tcgccgctga gatcgaccgc gatcacctgg tgccaggcct cgtcggtcat
     4261 cttgtgcagc atcgcgtcgc ggttgatccc cgcgttgttg acgagaatgt ccagccggcc
     4321 gtgcgtctca acgacctggg cgatcagcgc gtcacaggcc gcggagtcgc tcacgtcgag
     4381 agccatgccg tacgacgagc cgccgcactc cgccgcaacc ttgacggcat tctccgccac
     4441 gacatcggcg cacacgacga gggcctcgtc ggcggccaac cgtcgggcga tgccctcccc
     4501 gatgcctcgg ccggcgccgg tgactaccgc aatgcgatcg cgaaggcgtg acacatgcac
     4561 cgtctcctcg ttcgatcgat cgat
//
LOCUS       DATIBB010000171         5216 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_631917, whole genome shotgun sequence.
ACCESSION   DATIBB010000171 DATIBB010000000
VERSION     DATIBB010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 5216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5216
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_631917"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..321
                     /locus_tag="VLC50_06655"
     CDS             <1..321
                     /locus_tag="VLC50_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018829836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HSF27185.1"
                     /translation="VTQAHRIAREEIFGPVLSVLTFRTPAEAVEKANNTPYGLSAGVW
                     TEKGSRILWMAARLRAGVVWANTFNRFDPSSPFGGYKESGYGREGGRHGLAAYLRAGE
                     EVTR"
     gene            318..1181
                     /locus_tag="VLC50_06660"
     CDS             318..1181
                     /locus_tag="VLC50_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016575465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HSF27186.1"
                     /translation="MSPRLEVRKTYKLYVGGAFPRSESGRSYEVTDAKGRFLANAALA
                     SRKDARDAVVAARKAVPGWSGATAYNRGQVLYRVAELLEGRRDQFVAEVAAGEGLTPA
                     RARAAVDAAIDRWVWYAGWSDKIAQVAGGTNPVAGPYFDFSLPEPTGVVAVLAPQESS
                     LLGLVSVLAPVIVGGNTAVVLASEARPLPSVTLAEVLATSDVPAGVVNVLTGRAGEVA
                     PWLAAHMDVNAIDLTGADDALAVDLEVAAADNLKRVRRPSPGEDWSATPSLERMLWVL
                     ETKTVWHPIGI"
     gene            complement(1186..1647)
                     /locus_tag="VLC50_06665"
     CDS             complement(1186..1647)
                     /locus_tag="VLC50_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020700030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HSF27187.1"
                     /translation="MEINHVVVRRAVDTDAAAVAEVWLRSFKAAFPSVRRAHTDDQVR
                     SWFRDVVVPGMETWVATVEEAVVGMMVLDGEDLDQLYLDPAWQGRGIGDRLVEVAKHR
                     RPAGLELWTFQVNEPARRFYERHGFVAVECTDGHRNEEREPDIRYRWRPEK"
     gene            1720..2814
                     /locus_tag="VLC50_06670"
     CDS             1720..2814
                     /locus_tag="VLC50_06670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF27188.1"
                     /translation="MLIDARAVLVELVLPSGPGFGVRRSAGGPFVVEDQALVVYQTPS
                     DAGFADDAVPSERFDLVHAADRLTARRHPWTPELLDAVVLVHDLSAQLGLTGTHARTG
                     PGSRLRTVAAERRLDERIAADENRQLFELPTQWLAGLPTIELAAVTAEWLDALAALDA
                     ALRWVPGPPTLPRVVSVSLEELVPEVEVLWLGAAERGGFTLMAGADPMSDLPARFLGR
                     TGTVELYADVEALRAYLSDGAPRLPGVPSWHRLVDAGRVVDLTPYEDNVIDLDELGAT
                     LHRLTPTVADQLLRARLLIVDLADAVEAIDVLDRLADGPLARLFDRDLPVLAEDPRRA
                     ADRIAALDADLLGAEWAELVKALASRLSWR"
     gene            complement(2818..3357)
                     /locus_tag="VLC50_06675"
     CDS             complement(2818..3357)
                     /locus_tag="VLC50_06675"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020520892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HSF27189.1"
                     /translation="MTRLITVEDAPALTELVRRNRDFLAPWEPLRTDDYFTVDVQRAG
                     IEDALARHALGTTVPHVILDRSGEVVGRITLSGIVRGPFQSCSLGYWVDEAHNGQGLA
                     TAAVGEIVRHAFTDLKLHRVQAETLPHNAASQKVLARNGFARIGLAPHYLNIAGRWQD
                     HVVFQVVNAEPPGGALTAG"
     gene            complement(3402..4001)
                     /locus_tag="VLC50_06680"
     CDS             complement(3402..4001)
                     /locus_tag="VLC50_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HSF27190.1"
                     /translation="MTTDAATLRAAVLPRSSVALRSVADVTLVLGGAALITASALVSI
                     PLWFTPVPFTLQTFAVLAIGAALGSARGGLATLLYLMIGIVGFPVFAEGTNGVEVIFG
                     ATGGYLVGMLLAAVLVGRLAERRRMDRQLRTAVLAMVAGDAVILGLGTLWLAAALGVS
                     LEKAFVLGVAPFLVFEVVKVVAATVTLPAAWKAVNRLRG"
     gene            complement(4075..5178)
                     /locus_tag="VLC50_06685"
     CDS             complement(4075..5178)
                     /locus_tag="VLC50_06685"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007384299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009168 - purine ribonucleoside
                     monophosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HSF27191.1"
                     /translation="MSEIGPLPSHSQIRRAPKALLHDHLDGGLRPDTIVELAEETGYE
                     RLPATDAAALGRWFLDAASSGSLERYLETFAHTVGVMQTTDALVRVAAECAEDLAEDG
                     VVYAEVRYAPEQHLARGLTLPEVVEAVNEGFRLGERRALEAGRRIRIGTLLTAMRHAA
                     RSREIAELAVRYRDEGVVGFDIAGAEAGYPPTRHLDAFEYLQRENAHFTIHAGEAFGL
                     PSIWQAIQWCGADRLGHGVRIIDDVELRPDGRVALGRLASYVRDRRIPLEMCPSSNVQ
                     TGAAPSISEHPIGLLRQLNFRVTVNTDNRLMSATSMTMEMAKLVKGFGYHLDDLRWLT
                     INAIKSAFIPFDERLALIEDVVKPGYAALRAEV"
BASE COUNT          747 a         1890 c         1854 g          725 t
ORIGIN      
        1 gtcacccagg cccaccggat cgcccgcgag gagatcttcg gcccggtgct gtcggtgctg
       61 acgtttcgga ccccggccga ggcggtggag aaggccaaca acacgcccta cggcctgtcc
      121 gccggggtgt ggaccgagaa ggggtcgcgg atcctctgga tggccgcccg gctgcgcgcc
      181 ggggtcgtct gggccaacac gttcaaccgg ttcgacccga gctcgccgtt cggcggttac
      241 aaggagtccg gctacggccg cgagggcggc cggcacgggc tggccgccta cctgcgcgcc
      301 ggcgaggagg tcactcgatg agcccgcggc tcgaggtgcg caagacctac aagctctacg
      361 tcggcggggc gttcccgcgc agcgagtccg gccgctccta cgaggtcacc gacgcgaagg
      421 gccggttcct cgccaacgcg gcgctggcct cgcgcaagga cgcccgcgac gcggtggtgg
      481 ccgcccgcaa ggcggtgccc ggctggtcgg gcgcgacggc ctacaaccgc ggccaggtgc
      541 tctaccgggt cgccgagctg ctcgagggcc ggcgcgacca gttcgtcgcc gaggtcgccg
      601 ccggcgaagg gctgaccccg gcccgggcgc gggccgccgt cgacgccgcg atcgaccggt
      661 gggtctggta cgccggctgg tccgacaaga tcgcccaggt ggccggggga accaacccgg
      721 tggcagggcc ctacttcgac ttctcgctgc ccgagccgac cggcgtggtc gccgtgctcg
      781 ccccgcagga gtcgtcgctg ctcggcctgg tctcggtgct ggccccggtg atcgtcggtg
      841 gcaacacggc cgtcgtgctc gccagcgagg cccgcccgct gccgtcggtc acgctggccg
      901 aggtgctggc cacctccgac gtgcccgccg gggtggtcaa cgtgctcacc ggccgcgccg
      961 gcgaggtcgc cccgtggctg gccgcgcaca tggacgtcaa tgccatcgac ctcaccggcg
     1021 ccgacgacgc gctagcggtc gacctcgagg tggccgccgc cgacaacctc aagcgggtcc
     1081 gtcggccctc ccccggcgag gactggtcgg ccacgccgag cctcgagcgg atgctgtggg
     1141 tgctcgagac caagaccgtc tggcacccga tcggcatcta gcgcgctatt tttcaggccg
     1201 ccaccggtat cgaatgtccg gctcccgttc ctcgttgcgg tgaccgtcgg tgcactcgac
     1261 ggcgacgaaa ccgtgccgct catagaagcg acgtgccggc tcgttgacct ggaacgtcca
     1321 cagctccaga cctgccggac gccggtgctt cgccacctcc acgagacgat cgccaatacc
     1381 ccggccctgc caggctggat cgagatagag ctggtccagg tcttcgccgt ccagcaccat
     1441 catcccgacc actgcctcct cgacggtcgc cacccaggtc tccatgccgg gcacgaccac
     1501 gtcccggaac caggaccgga cctggtcgtc cgtatgagca cgacgcacac tcggaaacgc
     1561 tgccttgaag gaacgcagcc acacctcggc caccgccgcg gcgtcggtgt cgaccgcacg
     1621 tcggacgacg acatggttga tctccacagc cggcatgctc acagagacac agctccgcgg
     1681 caacgccaga tcgggccgcc gggggcttac cgtgctggcg tgctcatcga cgcgcgcgcg
     1741 gtgctggtcg agctggtgct gccctcgggc ccgggtttcg gcgtacgacg atccgctggc
     1801 ggtccgttcg tggtcgagga ccaggcactg gtcgtctatc agacgccgtc agacgcgggg
     1861 ttcgccgacg acgccgtgcc gtcggaacgc ttcgacctgg tccacgccgc cgaccggctc
     1921 accgcccgcc gccatccctg gacccccgag ctgctcgacg cggtggtgct cgtccatgac
     1981 ctgagcgccc agctggggtt gaccggcacc catgcgcgca ctggcccggg gtcacgactg
     2041 cgcacggtgg cggccgagcg gcgcctcgac gagcggatcg cggccgacga gaaccgtcag
     2101 ctcttcgagc tgccgaccca gtggctggcc gggctgccga cgatcgagct ggctgcggtg
     2161 accgccgagt ggctcgacgc gctggcggcc ctcgacgccg ccctgcgctg ggtgcccggg
     2221 ccgccgaccc tgccccgggt cgtctcggtg tcgctggagg agctcgtccc cgaggtggag
     2281 gtcctctggc tgggcgcggc cgagcgcggc gggttcaccc tgatggccgg cgcagacccg
     2341 atgagcgacc tgccggcccg gttcctgggc cgaaccggca cggtcgagct gtacgccgat
     2401 gtcgaggccc tgcgcgccta tctgtccgac ggagcgccgc gcctgcctgg tgtgccgtcg
     2461 tggcaccggc tggtcgacgc cggccgcgtg gttgacctca cgccctatga ggacaacgtg
     2521 atcgacctcg acgagctcgg cgcgacgctt caccggctga ccccgacggt ggccgaccag
     2581 ttgttgcggg cgcggctgct catcgtcgac ctggccgacg ccgtcgaggc catcgacgtg
     2641 ctcgatcggc tggctgacgg cccgctggcg cgcctgttcg accgcgacct gccggtgctg
     2701 gccgaggacc cccgccgtgc ggccgaccgg atcgccgccc tcgacgccga cctgctcggc
     2761 gccgagtggg ccgagctggt gaaggcgctc gcgagccggc tcagctggcg gtgagcctca
     2821 cccggcggtg agcgctccac cgggcggctc ggcgttgacc acctggaaga cgacgtggtc
     2881 ctgccaccgg cccgcgatgt tgaggtagtg cggggcaaga ccgatgcggg caaagccgtt
     2941 gcgcgccagc accttctgcg aggcggcgtt gtgcggcagc gtctccgcct ggacccggtg
     3001 cagcttgagg tcggtgaagg cgtgccggac gatctcgccg accgccgcgg tggcgagccc
     3061 ttgcccgttg tgcgcctcgt cgacccagta gcccaggctg catgactgga acggcccgcg
     3121 cacgatgccg ctgagcgtga tccggccgac cacctcaccc gaccggtcga ggatgacgtg
     3181 cggcacggtc gtgcccagcg cgtgtcgcgc gagtgcgtcc tcgatgccgg cgcgctgcac
     3241 gtcgaccgtg aagtagtcgt cggtccgcag cggctcccag ggcgcgagga agtcacggtt
     3301 gcgccgcacc agctcggtca gggcgggcgc gtcctcgacg gtgatcagtc gggtgatcgg
     3361 gtgcacccgc cccaccctgc cacccccgcc gcgtggtgcg atcagccgcg gagccggttg
     3421 acggccttcc aggcggccgg aagggtgacg gttgccgcga ccaccttgac gacctcgaac
     3481 acgaggaagg gcgccacgcc gaggacgaac gccttctcga gcgagacccc cagcgcggcg
     3541 gccagccaca gggtgcccag cccgaggatc accgcgtcgc cggcgaccat cgcgagcacc
     3601 gccgtgcgca gttggcgatc catccggcgc cgctcggcca gccggcccac gagcaccgcc
     3661 gcgagcagca tgcccacgag gtagcccccg gtcgcaccga agatcacctc gacgccattg
     3721 gtgccctcgg cgaagacggg gaagccgacg atcccgatca tcaggtagag cagcgtcgcc
     3781 aggccgcccc gcgcgctgcc cagcgcggcg ccgatcgcga gcacagcgaa cgtctgcagc
     3841 gtgaagggca ccggcgtgaa ccacagcggg atgctcacga gcgcagaagc ggtgatcagc
     3901 gccgcgccgc caaggacgag cgtgacgtcg gccacgctgc gcagggcgac cgaggaacga
     3961 ggcagtacgg cggcacgcag cgtggcggcg tcggtagtca cgtgagacct ccaggtgggg
     4021 ggacaaggcc gcagtcaacc taacggtgac cggccagtaa cagcagcagg cgtctcagac
     4081 ctcggctcgc aaggcggcgt aaccgggctt gacgacgtct tcgatgagcg ccagccgctc
     4141 gtcgaacggg atgaacgccg acttgatcgc gttgatcgtc agccagcgca ggtcgtcaag
     4201 gtggtagccg aatcccttga ccagcttggc catctccatg gtcatcgacg tggcgctcat
     4261 caaccggttg tcggtgttga cggtcacccg gaagttgagc tggcgcagca gcccgatcgg
     4321 gtgctcggag atcgacggag ccgccccggt ctgcacgttg gacgacgggc acatctccag
     4381 cgggatgcgc cggtcccgca cgtaggacgc cagccggccc aacgcgaccc gcccgtccgg
     4441 gcgcagctcg acgtcgtcga tgatgcgcac cccatggccg agccggtcgg cgccgcacca
     4501 ctggatggcc tgccagatcg agggcagccc gaacgcctcg ccggcgtgga tcgtgaagtg
     4561 ggcgttctcc cgctgcaggt attcgaacgc gtccaggtgc ctggtgggcg ggtagccggc
     4621 ctcggcgccg gcgatgtcga acccgacgac gccctcgtcg cgatagcgga cggccagctc
     4681 cgcgatctca cgagagcggg cggcgtggcg catcgcggtg agcagggtgc cgatccggat
     4741 ccgccggccg gcctccagcg cccgtcgctc gccgagccga aagccctcgt tgaccgcctc
     4801 gacgacctcg ggcagcgtca gcccgcgggc caggtgctgc tcgggcgcgt agcggacctc
     4861 ggcgtagacg acgccgtcct cggccaggtc ctcggcgcac tcggcggcca cccgcaccag
     4921 cgcgtcggtg gtctgcatca ccccgacggt gtgcgcgaac gtctcgagat agcgctcgag
     4981 cgagcccgag ctggcagcgt cgaggaacca ccggcccagc gccgcagcat cggtggcggg
     5041 caaccgctca tagccggtct cctcggccag ctcgacgatg gtgtccgggc gcagcccgcc
     5101 gtcgaggtgg tcgtgcagga gcgccttggg tgcgcgccgg atctgggagt ggctcggcag
     5161 cggtccgatc tcgctcacgg aggcgaacct accgggcagt acgacgcctg atggac
//
LOCUS       DATIBB010000172        15692 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_106217, whole genome shotgun sequence.
ACCESSION   DATIBB010000172 DATIBB010000000
VERSION     DATIBB010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 15692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15692
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106217"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..412)
                     /locus_tag="VLC50_06690"
     CDS             complement(<1..412)
                     /locus_tag="VLC50_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017587740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSF27192.1"
                     /translation="MTDRRVLPTSRRAGIGAAGLVAVLLVAASLRPAITSVGPVLDLI
                     GADTGLSQTALGLLGALPLLAFAVVSPLVPGPQRRLGLERMLLFALVVLAVGTVARSL
                     PTLPALWLGTALIGAAIAVGNVLLPSLIKRDFGGH"
     gene            411..1529
                     /locus_tag="VLC50_06695"
     CDS             411..1529
                     /locus_tag="VLC50_06695"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HSF27193.1"
                     /translation="MPARYAVRLVQVGAAQADESEVTGGPGTRRGTPWEAALPLVGAL
                     VAALMASYAVLHLLLLDGRAQIALATVATAAAVGLGATSALTWRRGVPAAALLPLSTT
                     LVVLLAAHAVLAMVTTGDLAWTSSVLLAIFAAGALLSRLRWLIGALYVTWGIWLAGLV
                     AVGPAPQWTVAAVSMLAATGLAVALQVSSARLLAALDSAAEMAEVVAVRDSLTGLANR
                     RGLQMLAAPIVEAARRQGDAVHAMFLVVDGLTAPDGAHQQPAGDELVLSVGEALTAVT
                     RATDVIARWETDTFVVAGPGSGMSPLELERRVRDRLIMVAALERGWSLRISAGAAMLA
                     PWDAGTLDSLLDDAAHDLTRRQTVRWPERRRDRRAAAE"
     gene            1686..4187
                     /gene="leuS"
                     /locus_tag="VLC50_06700"
     CDS             1686..4187
                     /gene="leuS"
                     /locus_tag="VLC50_06700"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012851942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HSF27194.1"
                     /translation="MTETEQAQQASRTDASYDVHAVQDKWEPVWDELAPFRSGRPDDP
                     RPRKYVLDMFPYPSGDLHMGHAEAYALGDVVARYWVQRGFNVMHPIGWDAFGLPAEGA
                     AIKRGVDPREWTYANIAQQRASMRRYACSFDWDRVLYTCDPGYYRWNQWLFLRLFEQG
                     LAYRKPSQVNWCPHDQTVLANEQVVGGRCERCDTPVTKKKLTQWYFKITDYADRLLDD
                     MAELEGHWPDKVLSMQRNWIGRSVGADVAFVIEGRDEPVTVYTTRPDTLYGATFFVVA
                     ADSDLAAELAADAPEDVREAFAAYVEQVRATSEIDRLSTEREKTGVFLQRYAVNPVNG
                     ERLPVYAADYVLADYGHGAIMAVPAHDQRDLDFARAFGLPVRVVVDTGAEDPAVTGVA
                     TPGDGALVNSGPLDGLGKAEAIARITEILREKGLGEAAVNYRLRDWLISRQRYWGTPI
                     PIVHCPVCGEVAVPDDQLPVRLPNMAGLDLTPKGTSPLGAATDWVNVACPRCGGPATR
                     DTDTMDTFVDSSWYYLRYVSPGRDDVAFDPELVRQWLPVDQYVGGVTHAILHLLYSRF
                     ITKVLHDQGYLDFVEPFTRLLNQGMVLMDGSAMSKSRGNLVRLSDELATHGVDAIRLT
                     MVFAGPPEDDIDWADVSASGSAKFLARAWRLAGDVTSAVGTDPTSGDLALRKATHHAV
                     HDAGEAVEQFRFNVAVARLMELVNATRKAIDSGVGPADPAVREAAEAIAVLLSLFAPY
                     TAEEMWARLGHEPTVALAGWPVVDETLLVQDHVTCVVQVAGKVRDRLEVAPSIGEAEL
                     RELALATPGVARALDGRGVRTVIVRAPKLVNVVPA"
     gene            4211..5062
                     /locus_tag="VLC50_06705"
     CDS             4211..5062
                     /locus_tag="VLC50_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014135715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HSF27195.1"
                     /translation="MPGHVAVVTDSTAYLPAELLARHPVTVVPLQVVVDGRAYDEGSG
                     EVDVAHALRRGACVTTSRPAPDVVLAAYLAAADAGASAVVSVHLSAEMSGTCAAAELA
                     ARDAPLPVRVVDSRSLGMGLGFAVLAALAAVRAGADLDAVAEAARCRAAAGRALFYVD
                     TLEYLRRGGRIGGARSLLGSALAVKPLLHLVDGAIEPLERVRTAGRARARLVDLTVER
                     AGQELVDVAVHHLAAPERAAEVAGLLRERIPALGELHVSEVGAVVGAHVGPGLLATVV
                     APRLLPA"
     gene            5295..6026
                     /locus_tag="VLC50_06710"
     CDS             5295..6026
                     /locus_tag="VLC50_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006237072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HSF27196.1"
                     /translation="MPQRPDAGPSTAPAAPAHRAGRHRRVPGQGHRVEVDGWAAAVLL
                     IVLGVGLAIGLAVLVRARPHALAVPDDPGAVTGSTAGATAGPWSTVGATASASPSSGP
                     STLVVHVAGLVRSPGVVTLTPGARVADAVVAAGGVSPGGDPATVNLARPVVDGEQIVV
                     RESGAPLPPAASGAGSAAAGAPLDLNGADQHALEQLPGVGPVLAGRILEWRTKHGRFS
                     SVDELREVSGIGAKVFAALADRVRV"
     gene            6023..8431
                     /locus_tag="VLC50_06715"
     CDS             6023..8431
                     /locus_tag="VLC50_06715"
                     /inference="COORDINATES: protein motif:HMM:NF015714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HSF27197.1"
                     /translation="MTTEAPDRSRPQPHDLRLVPAAVALWVATWVGLPLAARHGPLVG
                     LAVVAPVAGLAALGWLRHLRMVGLVATAAVVGLLVAGARGSADQAGPLPGLAGERAIA
                     RVEAVVATQPHLLVGQVTGDRRMPDQVSLDVTIRLVTARGHTTRVVTPARVVAAPTGW
                     AGLDPGDHVEANGRLALDSGHAVLLRATGPPVVVGGPPVWQRSANRIRHGLRTASAGL
                     PPDARALLPGLVVGDTSGLPTDLRDDFRTTGLAHLLAVSGGNLAIVIGAVLWAARWCG
                     VRGRWLVLLALTGMLAFVGVAGAEPSVLRAAVMGSIALLALFGGRRRHGLPAVAAAVL
                     VLLLADPRLARSYGFALSVLATAGLVLLARPWGRAFSGWLERRWDHPTWARLARLLGY
                     AVAVPLAAQVAVAPVLVLLAPQVSLVSVPANLLAAPAVPPATVLGLVAAVVSLVSAPA
                     AAVVAGLAGLPAAWIAGVGRLGARAPLGVLPWPAGVGGALLLTAVIGAAALVVRRGGP
                     RWWRRIRLTRRSRAVVGFTAAVVVTVGLRLPAVLSGPWPPPGWALVACDVGQGDALVL
                     PVAAATAVVVDVGPDPAAVEDCLDGLGVDRVALLVLTHLHADHVEGLPGVLSGRRVDQ
                     VLVGPYGEPAGEARRVRAWLAAADVPVRVAVPGERWSMGATRLQVLAPSRVIDEESIA
                     NNASIVLRADSAGVRLLLTGDVEPPAQRALLDGPLRAPDVVPVDVLKVAHHGSAAQLD
                     AVLAGVGARLAVVSVGAGNDYGHPAPSTLATLHRAGILVERTDRDGTIAVLGPRQRLR
                     VVEH"
     gene            8510..9502
                     /locus_tag="VLC50_06720"
     CDS             8510..9502
                     /locus_tag="VLC50_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004944405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HSF27198.1"
                     /translation="MPRAEPTDPPALTLVVGPEDLLVDRAVAGLVRAARALDVDTDVR
                     ELEAAGMAPGTVTGHTSPSLFGERTVIVVRGVQDAADALAQELAEVCATAGRGELDGV
                     HVVLVHKGGQRGKKLLEAARKAGAHEIACKEVKWDSDKSRFVEGEFRAARRRVTPEAV
                     RALVDAVGSDLRELAAACAQLAADTEGVVDAGVVDRYHGGRVEASGFKVADAAIEGRC
                     EDALSLVRHALATGVDPVPINAALGAGLRAVVKVAGARRGSPEELARELGMAPFQVKK
                     ARGQLRGWSPEGIAAAIDAVAWADAQIKGAGADPLYALERTVVAVARARTPRDS"
     gene            complement(9650..9919)
                     /gene="rpsT"
                     /locus_tag="VLC50_06725"
     CDS             complement(9650..9919)
                     /gene="rpsT"
                     /locus_tag="VLC50_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016443759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HSF27199.1"
                     /translation="MANIKSQIKRIRTNEERRLRNKAVKSELKTAVRRVREAAAAGEP
                     DKAADLLRAASRKLDKAVSKGVIHKNQAANRKSALAKSLVKTPQA"
     gene            10156..12018
                     /gene="lepA"
                     /locus_tag="VLC50_06730"
     CDS             10156..12018
                     /gene="lepA"
                     /locus_tag="VLC50_06730"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019877935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HSF27200.1"
                     /translation="MPPTTNAPRPAATPPELIRNFSIIAHIDHGKSTLADRMLQLTGV
                     IDERSMRAQYLDRMDIERERGITIKSQAVRIPWEVDGRAHVLNLIDTPGHVDFTYEVS
                     RSLAACEGALLLVDAAQGIEAQTLANLYLALENDLTIIPVLNKIDLPAAQPDKYAAEL
                     ARLIGCEPSDCARVSGKTGAGVGELLDRIVREVPPPGGDADAPARAMIFDSVYDTYRG
                     VVTYVRVIDGELRARERILMMSTKATHELLEIGVSSPEPTPCDGLGVGEVGYLITGVK
                     DVRQSKVGDTVTTANRPATEALSGYRDPKPMVFSGLYPIDGSDYPELREALDKLKLND
                     AALVYEPETSAALGFGFRCGFLGLLHLEIVRERLEREFGLDLISTAPNVVYRVVMEDG
                     TEHVVTNPSEFPAGKIDQVHEPMVRATILTPSDYVGAVMELCQSRRGTLLGMDYLSED
                     RVELRYTLPLAEIVFDFFDALKSRTRGYASLDYEPTGEQVAALVKVDILLHGDPVDAF
                     SSIVHKDKAYAYGVAMTGRLRELIPRQQFEVPIQAAIGSRVIARETIRAIRKDVLAKC
                     YGGDITRKRKLLEKQKEGKKRMKMVGRVEVPQEAFIAALSTSDPSAAGDKATRK"
     gene            12501..14333
                     /locus_tag="VLC50_06735"
     CDS             12501..14333
                     /locus_tag="VLC50_06735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003976243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HSF27201.1"
                     /translation="MSGTLTESTAGLAARPASVARMFLDRVAASPQREAFRYRVGEQW
                     RSLSWSETADRVSALSAGLLSIGLGHQDRVAIASSTRIEWILADLAIMCAGGATTTIY
                     PSTIEEDVAFILADSGSRVAVAEDAVQLAKIQAVWDRLPELRHVVLLDLDHDEDDERI
                     ITFAELEARGREHLASDPQVVERASAAVGPEDLATLIYTSGTTGRPKGVRLVHDCWTY
                     EGVAIDSLGILGPDDLQYLWLPLSHSLGKVLQVMQLTCGFATAVDGDMERIVDNLAII
                     RPTFMGAAPRIFEKVHSRVVTMVDHEGGAKAKIFHWAVGVGIKASRVKEAGGRPSGLL
                     AVQNALADRLVFAKLRQRFGGRIRYFISGSASLSKDIAEFFNAAGMLILEGYGLTETS
                     AASFVNRPQRYRFGTVGLPMPGTEVRIADDGEILVRGPGVMRGYHQLPEQTAEAFTDD
                     GWFKTGDIGELDDGFLRITDRKKDLIKTSGGKYVAPQAIEALFAAVCPYASYIVVHGD
                     RRNYVTALVTLDRESLTGWATTQGWGERPYEDLIASAEVHAMVEGYLAEVNAKLARWE
                     TIKKFTILPDELSVDTGDLTPSMKLRRKAVEAKYLTVLDAMYEN"
     gene            14357..15589
                     /gene="hemW"
                     /locus_tag="VLC50_06740"
     CDS             14357..15589
                     /gene="hemW"
                     /locus_tag="VLC50_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016468924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HSF27202.1"
                     /translation="MPSTLPDGEPAPDDGRLPAAALAGLGQAPFGVYLHVPFCTTRCG
                     YCDFNTYTAAELVAPDGTTVSPTSYVETALAELRLARTVLGDAVPPATTVFVGGGTPT
                     LLPATDLGRLLDGVRRELGLAADVEVTTEANPESVDAAYLATLREVGFTRVSFGMQSA
                     RPHVLAVLERRHTPGRPEQCVAWARAAGFEHVNLDLIYGTPGESDDDWRASVQAAIGA
                     GPDHVSAYSLIVEPGTRLAAQVRRGQLAMPDDDVLADRYLIADELLSAAGLHWYEVSN
                     WATSPAARSRHNLLYWTGADWWGVGPGAHSHVGGTRWWNVKHPAAHAARLAAGRSPAQ
                     AREVLSEEDRYVERVLLGSRLSDGLALAELREPGRTAAAQARDDGLLERSSYDEGRAV
                     LTLRGRLLGDAVVRDLLD"
BASE COUNT         1993 a         5732 c         5803 g         2164 t
ORIGIN      
        1 cgtgcccgcc gaagtcacgc ttgatgagcg agggcagcag cacgttgccc accgcgatgg
       61 cggcgccgat cagcgccgta cccagccaca gcgccggcag ggtcgggagc gagcgcgcca
      121 cggtcccgac cgccaggacc acgagagcaa acagcagcat gcgctcgagg cccagccggc
      181 gctgcgggcc gggcacgagt ggagacacca cggcgaacgc cagcaacgga agcgcaccca
      241 gcaggcccag cgcggtctgg ctgagtccgg tgtcggcgcc gatgaggtcg aggaccgggc
      301 cgaccgacgt gatcgccgga cgcagcgagg cggccaccag cagtacggcg accaggcccg
      361 cggcgccgat gccggcgcgt cggctcgtgg gcaggacgcg ccggtcggtc atgcccgcac
      421 gctacgcggt caggttggtt caggtcggcg ccgcccaggc cgatgagagc gaggtgacgg
      481 gcggtccggg cacacggcgt ggcacgccct gggaggcggc gctaccgctg gtcggcgccc
      541 tcgtggcggc gctcatggcg tcgtacgccg tcctgcacct gctcctgctt gatggccgag
      601 cgcagatcgc cctggccacg gtcgcgaccg ccgcggcggt cggcctcggc gccacctccg
      661 ctctgacctg gcggcgcggg gtccctgccg cggcgctgtt gccgctgtcc accaccctgg
      721 tggtcctgct cgcggcgcac gccgtgctgg ccatggtgac caccggagac ctggcctgga
      781 cctcgagtgt gctgctggcg atcttcgcgg ccggcgcgct gctgtctcgg ctgcgctggc
      841 tcatcggggc gctctacgtc acctggggca tctggctggc ggggctcgtc gcggtcggcc
      901 cggcgccgca gtggaccgtc gcggccgtgt cgatgctggc cgccaccggg ttggcggtcg
      961 ctctgcaggt cagctccgcc cggctgctcg ccgcgctcga cagcgcagcc gagatggccg
     1021 aggtggtcgc ggtacgcgac tcgttgaccg ggctggccaa ccgccgcggc ctgcagatgc
     1081 tggccgcccc gatcgtcgag gccgcgcggc ggcagggcga tgcggtgcac gcgatgttcc
     1141 tcgtcgtcga cggactgacc gcgccggacg gggcgcacca gcagccggcc ggggacgagc
     1201 tcgtcctctc ggtcggagag gcgctcaccg cggtgacccg ggcgaccgat gtgatcgccc
     1261 gctgggagac cgacaccttc gtcgtggccg gccccgggtc gggcatgagc ccgctcgagc
     1321 tggaacgacg ggtgcgcgac cggctcatca tggtggcggc gctcgagcgt ggctggagcc
     1381 tgcggatcag tgccggggcc gccatgctgg ccccgtggga cgccggcacc ctggacagcc
     1441 tgctcgatga cgccgcccat gacttgacgc gccggcagac ggtgcgctgg ccggagcgtc
     1501 gccgcgaccg gcgggcggcc gctgagtgag ctctccgcta ccctgcacgt gtgcgcatcg
     1561 agcagctgct ccttagcggg ccgcgctgac cgccggtcga tgacgaccac gtcagcgcgg
     1621 cgacccctcg tggcccgagg ggttttttgt tgcccgtgcg acgacgatga cccgacgagg
     1681 acgagatgac cgagaccgag caggcgcagc aggcgagccg gaccgacgcg tcgtacgacg
     1741 tgcatgcggt gcaggacaag tgggagccgg tctgggacga gctggcgccg ttccgcagtg
     1801 ggcgccccga cgacccacgg ccgcgcaagt acgtcctcga catgttcccc tacccgtcgg
     1861 gcgacctgca catgggccac gccgaggcct acgcgctcgg tgacgtggtc gcccgctact
     1921 gggtccagcg cgggttcaac gtcatgcacc cgatcggctg ggacgcgttc gggctgcccg
     1981 ccgagggcgc cgcgatcaag cgcggcgtcg acccgcgcga gtggacctac gccaacatcg
     2041 cgcagcagcg ggcctcgatg cgccgctatg cctgctcgtt cgactgggac cgggtgctct
     2101 acacctgcga cccgggctac taccggtgga accagtggct gttcctgcgg ctgttcgagc
     2161 aggggctggc ctaccgcaag ccctcgcagg tcaactggtg cccgcacgac cagaccgtgc
     2221 tggccaacga gcaggtcgtc ggcgggcgct gcgagcgctg cgacacaccg gtgaccaaga
     2281 agaagctcac ccagtggtac ttcaagatca ctgactacgc cgaccggctg ctcgatgaca
     2341 tggccgagct cgagggtcac tggcccgaca aggtgctgtc gatgcagcgc aactggatcg
     2401 gtcgttcggt tggcgccgac gtcgcgttcg tcatcgaggg ccgcgacgag ccggtcaccg
     2461 tctacaccac ccgccccgac acgctctacg gcgccacgtt cttcgtcgtg gccgccgact
     2521 ccgacctggc cgccgagctg gccgccgacg cgcccgagga cgtgcgcgag gcatttgcgg
     2581 cgtacgtcga gcaggtgcgc gccaccagcg agatcgaccg gctgtccacc gagcgggaga
     2641 agacgggggt gttcctgcag cgctacgccg tcaaccctgt caacggcgag cggctgccgg
     2701 tctacgccgc cgactacgtg ctggccgact acggccacgg cgcgatcatg gcggtgccgg
     2761 cgcacgacca gcgtgacctc gactttgccc gcgccttcgg gctgccggtg cgggtggtcg
     2821 tcgacaccgg cgcggaggac ccggcggtca ccggggtggc cacccccggc gacggcgcgc
     2881 tggtcaactc cggcccgctc gacgggctgg gcaaggccga ggcgatcgct cggatcaccg
     2941 agatcctgcg cgagaagggg ctgggcgagg ctgcggtcaa ctaccggctg cgcgactggc
     3001 tgatctcccg gcagcgctac tggggcaccc cgatcccgat cgtgcactgc ccggtctgcg
     3061 gcgaggtcgc cgtacccgat gaccagctgc cggtgcggct gcccaacatg gcaggcctcg
     3121 acctgacccc caagggcacc tcgccgctgg gtgccgccac cgactgggtc aacgtcgctt
     3181 gcccgcgctg cggcggaccg gccacccgcg acaccgacac gatggacacc ttcgtcgact
     3241 cgtcgtggta ctacctgcgc tacgtctcgc cgggccggga cgacgtggcg ttcgaccccg
     3301 agctggtgcg ccagtggctg ccggtcgacc agtacgtcgg cggggtgacc cacgcgatcc
     3361 tgcacctgct ctacagccgg ttcatcacca aggtgctgca cgaccagggc tacctcgact
     3421 tcgtcgagcc gttcacccgg ctgctcaacc agggcatggt cctcatggac ggctcggcga
     3481 tgagcaagtc gcgcggcaac ctggtgcgcc tgtccgacga gctggccacc cacggcgtcg
     3541 acgcgatccg gctgaccatg gtcttcgccg gcccgcccga ggacgacatc gactgggccg
     3601 acgtgtccgc gtctggctcg gcgaagttcc tggctcgggc ctggcggctg gccggtgacg
     3661 tgacctcggc cgtgggcacc gacccgacga gcggtgacct cgcgctgcgc aaggcgaccc
     3721 accacgcggt gcacgacgcc ggcgaggccg tcgagcagtt ccggttcaac gtcgcggtgg
     3781 cccggctcat ggagctggtc aatgccaccc gcaaggcgat cgactccgga gtcggcccgg
     3841 ccgaccccgc ggtgcgcgag gcggccgagg cgatcgcggt gctgctgtcg ctgttcgcgc
     3901 cctacaccgc cgaggagatg tgggcccggc tcgggcacga gccgaccgtg gccctcgccg
     3961 gctggccagt cgtcgacgag acgctgctgg tgcaggacca cgtcacctgc gtcgtccagg
     4021 tggccggcaa ggtccgcgac cggcttgagg tggcgccctc gatcggcgag gccgagctgc
     4081 gcgagctggc cctggccacg ccgggggtgg cccgcgcgct ggacgggcgt ggtgtgcgca
     4141 ccgtgatcgt gcgcgcgccc aagctggtca acgtggtccc ggcctgatcg cgcccggcta
     4201 gcctgccgcc atgccgggac acgtcgcggt ggtcacggac tccaccgcct acctcccggc
     4261 cgagctgctc gcccggcacc cggtcaccgt cgtaccgctg caggtggtcg tcgacgggcg
     4321 ggcctacgac gagggcagcg gtgaggtcga cgtcgcccac gccctgcggc gcggtgcctg
     4381 cgtcaccacc tcccgcccgg ctccggacgt ggtgctggcg gcctacctcg cggccgccga
     4441 cgccggggcg agcgccgtgg tctcggtgca cctgtccgcg gagatgtccg gcacctgcgc
     4501 ggcggccgag ctggccgccc gcgacgcacc gctgccggtc cgggtcgtcg actcccgctc
     4561 gctggggatg ggcctgggct tcgctgtgct cgcggccctg gcggccgtcc gggcgggcgc
     4621 cgacctcgat gctgtcgccg aggcggcccg gtgccgggcg gcggcaggca gggcgctctt
     4681 ctacgtcgac acgctcgaat acctccggcg cggcgggcgc atcggcgggg cccgcagcct
     4741 gctcgggtcg gcgctggcgg tgaagccgtt gctgcacctg gtcgacgggg ccatcgagcc
     4801 gctcgagcgg gtgcgcacgg ccggccgggc ccgggcccgg ctcgtcgacc tcaccgtcga
     4861 acgcgccggg caggaattgg tcgacgtggc ggtgcaccac ctggccgcgc ccgagcgggc
     4921 cgccgaggtc gccggcctgc tgcgcgagcg gatcccggcg ctcggtgagc tccatgtcag
     4981 cgaggtcggc gccgtcgtcg gggcgcacgt ggggcccggc ctgctcgcca ccgtggtcgc
     5041 cccgcgcctg cttcccgcct gaccgccttt cgtccgtgat caagcaatcg ctgcccgccc
     5101 cgcgtccgac ccgtccacag gaggcggcga gcgcccggtt gtccacagcg ccgtacgacg
     5161 accgagggtg accccgggcc gccgcctagc gtcgcggggt ggacctgccc tggcggcaac
     5221 gccgacgcga gcgcgccgcg cggacggcgc ggcggctcct ggcggtcgtg ccggcgcccc
     5281 ggccgggtgg ctgggtgccg cagcgtccag acgccggccc gtcgacggct cctgccgcac
     5341 cggcccaccg agccggccgt caccggcgcg tgccggggca ggggcaccgg gtcgaggtcg
     5401 acggctgggc cgccgccgtg ctgctcatcg tgctcggggt cggcctggcc atcgggttgg
     5461 ccgtgctcgt gcgtgcccgg ccgcacgccc tcgccgtgcc cgatgacccg ggcgcggtca
     5521 ccggatccac ggccggggcc acggccgggc cctggtcgac ggtcggcgcg acggcatcgg
     5581 cctcgccgag ctccggcccg tccacgctgg tcgtccacgt cgccgggctc gtgcggtcgc
     5641 cgggggtggt cacgttgacg ccgggcgcga gggtggccga cgcggtggtc gccgccggag
     5701 gggtcagccc cggcggcgac ccggccaccg tcaacctggc ccgccccgtc gtcgacggcg
     5761 agcagatcgt cgtccgggag agcggcgcgc cgctgccgcc ggccgcgagc ggcgccggca
     5821 gcgcggcggc cggtgcgccg ctcgacctca acggcgccga ccagcacgcc ctcgagcagc
     5881 tccccggggt gggtccggtg ctggccggcc ggatcctcga gtggcgcacc aagcacggca
     5941 ggttctccag cgtcgacgag ctgcgcgagg tgtccgggat cggcgcgaag gtcttcgccg
     6001 ctctggccga ccgagtccgg gtgtgacgac cgaggcgccg gaccggtcgc gcccacaacc
     6061 tcatgacctg cggctggtcc cggccgccgt cgcgctgtgg gtcgccacct gggtcgggct
     6121 gccgctggcc gcacgccacg gccctctcgt cgggctggcc gtggtcgcgc cggtcgccgg
     6181 gctggccgcc ctcggctggc tgcgccatct gcggatggtg ggcctggtgg ccacggccgc
     6241 cgtggtcggc ctgctcgtcg cgggcgcccg tggctcggcc gaccaggccg gcccgctgcc
     6301 cggcctggcc ggcgaacgtg ccatcgcccg ggtcgaggcc gtcgtggcca cccagccaca
     6361 cctgctggtg ggtcaggtga cgggcgaccg gcggatgccc gaccaggtca gcctcgatgt
     6421 gacgatccgg ctggtcacgg cccgcgggca caccacccgg gtggtgaccc cggctcgcgt
     6481 ggttgccgcc cccaccggct gggccgggct cgaccccggc gatcacgtcg aggcgaacgg
     6541 acggctggcg ctcgactccg gccacgccgt gctgctgcgg gccaccggcc cacccgtggt
     6601 cgtgggtggg ccgccggtct ggcagcggtc ggccaaccgg atccgccacg ggctgcgcac
     6661 cgcctcggcc ggtctgcccc ccgatgcccg agcgctgctg cccggactgg tcgtcggcga
     6721 cacctccggc ctgcccaccg acctgcgcga cgactttcgc accaccgggc tggcgcacct
     6781 gctcgcggtc tccggcggca atctggcgat cgtcatcggt gcggtgctgt gggccgcccg
     6841 ctggtgcggg gtccgcggcc gctggctggt gctgctggcc ctcaccggca tgctcgcctt
     6901 cgtcggggtg gccggggccg agcccagcgt gctgcgggcg gccgtgatgg ggtcgatcgc
     6961 gctgctcgcg ctgttcggcg gtcggcgccg ccacggcctg ccggcagtgg ccgcagcggt
     7021 gctggtcctc ctgctcgccg acccgcggct ggcccgctcc tacgggttcg cgctgtcggt
     7081 gctggccacg gccgggctgg tcctgctggc ccgaccctgg gggcgggcat tcagcggatg
     7141 gctggagcgt cgatgggatc accccacctg ggcgcggctg gcccgcctgc tcggctacgc
     7201 ggtcgccgta ccgctggccg cccaggtcgc cgtcgctccg gtgctggtgc tgctggcgcc
     7261 ccaggtcagc ctcgtgtcgg tgccggccaa cctgctggcc gccccggcgg tcccacccgc
     7321 cacggtgctc gggctcgtcg cggcagtcgt ctcgctcgtc tcggcacccg cagccgccgt
     7381 ggtcgccggg ctggccgggc tgccggcggc ctggatcgcg ggcgtgggcc gcctcggcgc
     7441 acgggcaccg ctcggggtgc tgccctggcc ggccggggtc gggggggcgc tgctgctgac
     7501 cgcggtgatc ggggcagcgg ctctcgtcgt acgacgtggg ggcccgcgct ggtggcgccg
     7561 gatcaggctg acccggcggt cccgggccgt ggtcgggttc accgccgcgg tcgtcgtgac
     7621 ggtcgggctc cggctgcccg cggtgctgtc cgggccgtgg ccgccgcccg ggtgggccct
     7681 ggtcgcctgc gacgtcgggc agggcgacgc gctggtgctg ccggttgccg cggccaccgc
     7741 ggtggtcgtc gacgtggggc cggacccggc cgccgtcgag gactgcctcg acgggctcgg
     7801 ggtggatcgg gtggcgctgc tggtgctgac ccacctgcac gccgatcacg tcgaggggct
     7861 gcccggcgtg ctgtccgggc ggcgggtcga ccaggtgctg gtcggaccct acggtgagcc
     7921 ggccggcgag gcccgccggg tgcgggcctg gctggcggcg gccgacgttc cggtgcgggt
     7981 cgccgtcccg ggggagcggt ggagcatggg cgccacccgg ctgcaggtgc tggcacccag
     8041 ccgggtcatc gacgaggagt cgatcgccaa caacgcgagc atcgtgctga gggccgactc
     8101 ggccggggtg cgcctgctgc tcaccgggga cgtcgagcca cccgcccagc gggcgctgct
     8161 cgacggcccg ttgcgggcgc cggacgtcgt gccggtggac gtgctcaagg tggcgcacca
     8221 cgggtcggcc gcccagctcg acgcggtgct cgctggtgtc ggcgcccggc tcgcggtggt
     8281 cagcgtcggg gccggcaacg actacggcca cccggcgccc agcaccctcg cgacgctgca
     8341 tcgggccggc atcctcgtcg agcgcaccga ccgcgacggc acgatcgcgg tgctgggtcc
     8401 tcgacaacgg ctgcgcgtcg tcgagcactg agcagcggcc ccgcgccctc acgttggcgg
     8461 gcaccgagca ccgaacgggc ccgtgggacc cgcgtgccat gctgaccgcg tgccgcgcgc
     8521 tgagcccacc gaccctcccg ccctgaccct ggtcgtcggc ccggaagacc tgttggtcga
     8581 ccgggcggtg gcggggctgg tgcgtgcggc ccgagcgctc gacgtcgaca ccgacgtgcg
     8641 cgagctcgag gcggccggga tggcgccggg caccgtgacc gggcacacca gcccgtcgtt
     8701 gttcggcgag cgcacggtga tcgtggtccg gggcgtgcag gacgccgccg atgcgctggc
     8761 ccaggagctc gccgaggtgt gcgccacggc gggccggggc gagctcgacg gcgtgcatgt
     8821 cgtgctcgtg cacaagggtg gtcagcgcgg caagaagctg ctcgaggccg cccgcaaggc
     8881 gggcgcgcac gagatcgcct gcaaagaggt caagtgggac agcgacaagt cccgcttcgt
     8941 cgagggggag ttccgcgccg ccagacgccg ggtgacccct gaggcggtgc gcgccctggt
     9001 cgacgccgtc ggcagcgacc tgcgcgagct ggcggccgcg tgcgcccagc tggcggccga
     9061 caccgagggg gtcgtggacg ccggcgtcgt cgaccgctac cacggcggcc gggtcgaggc
     9121 cagcgggttc aaggtggccg acgcggcgat cgagggccgc tgcgaggacg ccctctcgct
     9181 ggtgcgacac gccctggcca ccggcgtcga cccggtgccg atcaacgccg cgctgggggc
     9241 cggcctgcgc gccgtcgtca aggtcgcggg cgcccgccgg ggctcaccgg aggagctggc
     9301 ccgcgagctg ggcatggcgc cgttccaggt caagaaggcg cgcgggcagc tgcgcgggtg
     9361 gagccccgag ggcatcgccg cggccatcga cgcggtcgcc tgggccgacg cgcagatcaa
     9421 gggcgccggg gccgatccgc tctacgcgct cgagcgcacc gtcgtcgcgg tcgcccgggc
     9481 ccgcactccc cgcgacagct aggcaaggcg ttgtggcact aggcaaggcg cgcacgcctc
     9541 gccaagcgcc acgacgccca cgtggcgtgg tcaatcgcac cgggtcgtgg gtgcgcgaag
     9601 gcccgggtct cccgtcggga gacgccgggc cttcggcgaa gcgggtgggt caggcctgcg
     9661 gggtcttgac cagcgacttg gccagcgcgg acttgcggtt ggcggcctgg ttcttgtgga
     9721 tgacgccctt gctcacggcc ttgtcgagct tgcggctcgc ggcgcggagc aggtcggcgg
     9781 ccttgtcggg ctcaccggct gcggcggcct cgcggacccg ccgtacggcc gtcttgagct
     9841 cgctcttgac cgccttgttg cgcaggcgcc gctcctcgtt ggtgcggatg cgcttgatct
     9901 gggacttgat gtttgccacg gaaagtctct ctgcatcgtc ggacaggtcg ggtgagggcg
     9961 ctcgaccggc ccgagactga cggtggtggg gacaccggtg ggacgggctg gccggtgcgc
    10021 gtcgacctgg gcgcgcacgc gacctgcgag attaccagcg cccggcgccc gggcccaatc
    10081 acgggtggga gcgtgggacg atggggcact cccgagcccg aacccagccc tctcatgaag
    10141 acgaggtagt ccagcgtgcc gccgacgacg aacgcgccgc gccccgcggc cacgccgccg
    10201 gagctcatcc gcaacttcag catcatcgcc cacatcgacc acggcaagtc gacgctggcc
    10261 gaccggatgc tgcagctgac cggggtgatc gacgagcggt cgatgcgggc gcagtacctc
    10321 gaccgcatgg acatcgagcg cgagcgcggc atcacgatca agtcgcaggc cgtgcgcatc
    10381 ccgtgggagg tcgacgggcg ggctcacgtg ctcaacctga tcgacacgcc cgggcacgtc
    10441 gacttcacct acgaggtgtc tcggtcgctg gccgcctgcg agggcgcgct gctgctcgtc
    10501 gacgcggccc agggcatcga ggcgcagacg ctggccaacc tctacctggc gctcgagaac
    10561 gacctgacca tcatcccggt gctcaacaag atcgacctgc cggcggccca gcccgacaag
    10621 tacgccgccg agctggcccg cctgatcggc tgcgagccct ccgactgcgc ccgggtctcc
    10681 ggcaagaccg gcgccggggt gggcgagctg ctcgaccgga tcgtgcgcga ggtcccgccg
    10741 cccggtggcg acgccgacgc gccagcccgc gcgatgatct tcgactcggt ctacgacacc
    10801 taccgcggtg tggtcaccta tgtccgggtc atcgacgggg agctgcgcgc ccgcgaacgc
    10861 atcctcatga tgtcgaccaa ggccacccac gagctgctcg agatcggggt cagctcaccc
    10921 gagccgacac cgtgcgacgg gctgggcgtc ggcgaggtcg gctacctcat caccggggtc
    10981 aaggacgtgc gccagtccaa ggtcggcgac accgtcacca cggccaaccg cccggcgacc
    11041 gaggcgctgt ccggctaccg cgaccccaag ccgatggtgt tctccgggct ctaccccatc
    11101 gacggctccg actaccccga gctgcgcgag gcgctcgaca agctcaagct caacgacgcg
    11161 gcgctggtct acgagccgga gacctcggcg gcgctcgggt tcggcttccg gtgcgggttc
    11221 ctcggcctgc tccacctcga gatcgtccgc gagcgcctcg agcgcgagtt cggcctcgac
    11281 ctgatctcga ccgcacccaa cgtggtctac cgggtggtca tggaggacgg caccgagcac
    11341 gtggtcacca accccagcga gttcccggcc ggcaagatcg accaggtgca cgagccgatg
    11401 gtgcgggcca cgatcctcac cccgagcgac tacgtcggcg ccgtcatgga gctgtgccag
    11461 agccggcgcg gcacgctgct gggcatggac tacctgtccg aggaccgggt cgagctgcgc
    11521 tacacgctgc cgctggccga gatcgtcttc gacttcttcg acgcgctcaa gtcgcgcacc
    11581 cgcggctatg ccagcctcga ctacgagccc accggcgaac aggtggcggc gctggtcaag
    11641 gtcgacatcc tcctgcacgg cgacccggtc gacgccttca gctcgatcgt gcacaaggac
    11701 aaggcctatg cctacggcgt cgcgatgacc gggcggctgc gcgagctgat cccgcgccag
    11761 cagttcgagg tgccgatcca ggccgcgatc ggctcgcggg tgatcgcccg cgagacgatc
    11821 cgggcgatcc gcaaggacgt gctggccaag tgctacggcg gtgacatcac ccgcaagcgc
    11881 aagctgctgg agaagcagaa ggagggcaag aagcggatga agatggtcgg ccgggtcgag
    11941 gtgccgcagg aggccttcat cgccgcgctg tcgaccagcg acccgtccgc ggccggcgac
    12001 aaggccaccc gcaagtaggc ggatccccgc tccgcccgcc cggcgccctc gctcctcgcc
    12061 cgcccccggc tcgcctccct cgtgatcatg cacttgtgca cgcgatcatg cggttgtgca
    12121 cgcgattgtg gaaggttgcc gcgccaggac ctgcacaaag tgacacggtc tcgggtccgg
    12181 catgatcacg aacagcgccg tggcctgcac aactgcatga tcaccgctgg gggtcggggg
    12241 ccagggccgt tgcacacgtg acacccctgg ctggtgatca tgcacgtatg cgccgcacac
    12301 ctgcatgatc acggtcgggg tggggtcggg ggccaggggc cgtgcacagg tgcatgatca
    12361 ccaacagagg gggcggcggc gggggagtcg cgcaacgcct cgaagcggtc taaccctccc
    12421 catgcgcggc cgttccaagt acgttcgggg cacacaccgt gccgctgggc cgagaccggg
    12481 ccgcgagatg gaggatggcg atgtcaggca ccctcaccga gtcgacggcg gggcttgccg
    12541 cccggcccgc ctccgtggcc cggatgttcc tcgaccgggt cgccgcctcg ccccaacgcg
    12601 aggcgttccg ttaccgcgtc ggtgagcagt ggcgctcgtt gtcgtggtcg gagaccgccg
    12661 accgggtcag cgcgctgtcg gcggggctgc tctcgatcgg cctcggccac caggaccggg
    12721 tcgccatcgc gtcctccacg cgcatcgagt ggatcctcgc cgacctggcg atcatgtgtg
    12781 ccggcggtgc gaccacgacg atctacccgt cgacgatcga ggaggacgtg gccttcatcc
    12841 tggccgactc cggctcgcgg gtggccgtcg ccgaggacgc cgtacagctg gccaagatcc
    12901 aggcggtctg ggaccggctg cccgagctgc gccacgtcgt cctgctcgac ctcgaccacg
    12961 acgaggacga cgagcgcatc atcacgttcg ccgagctcga ggcccgcggg cgcgagcacc
    13021 tggcctccga cccgcaggtg gtcgagcggg cctcggcagc ggtcggcccc gaggacctgg
    13081 ccaccttgat ctacacgtcg ggcaccaccg ggcggcccaa gggggtccgg ctggtccacg
    13141 actgctggac ctacgagggc gtcgccatcg actcgctggg gatcctgggc cccgacgacc
    13201 tgcaatacct ctggctgccg ctgtcgcact cgctcggcaa ggtgctgcag gtcatgcagc
    13261 tcacctgcgg gttcgcgacc gccgtcgacg gcgacatgga gcgcatcgtc gacaacctcg
    13321 ccatcatccg cccgacgttc atgggcgccg cgccgcgcat cttcgagaag gtgcactccc
    13381 gggtggtgac gatggtcgac cacgagggcg gggccaaggc caagatcttc cactgggcgg
    13441 tcggcgtcgg gatcaaggcc tcccgggtca aggaggccgg cggacggccc tccggcctgc
    13501 tcgccgtaca gaatgccctg gccgacaggc tggtcttcgc caagctgcgc cagcggttcg
    13561 gcgggcggat ccgttacttc atctccggct cggcgtcgct gtccaaggac atcgccgagt
    13621 tcttcaacgc cgccgggatg ctgatcctcg agggctacgg gctgaccgag accagcgcgg
    13681 cctccttcgt caaccgcccg cagcgctacc ggttcggcac ggtggggctg ccgatgccgg
    13741 gcaccgaggt cagaatcgcc gacgacgggg agatcctggt ccgcgggccc ggcgtgatgc
    13801 gcggctacca ccagctgccc gagcagaccg ccgaggcgtt caccgacgac ggctggttca
    13861 agaccggcga catcggcgag ctcgacgacg ggttcctgcg catcaccgac cgcaagaagg
    13921 acctgatcaa gacctccggc ggcaagtacg tcgccccgca ggcgatcgag gcgctcttcg
    13981 ccgcggtctg cccctacgcc agctacatcg tcgtgcacgg cgaccggcgc aactacgtca
    14041 ccgcgctggt caccctcgac cgggagtcgc tgaccgggtg ggccaccacg cagggatggg
    14101 gcgagcggcc ctacgaggac ctgatcgcct ccgcggaggt ccacgcgatg gtcgagggct
    14161 acctggccga ggtcaacgcc aagctggccc gctgggagac gatcaagaag ttcacgatct
    14221 tgcccgacga gctcagcgtc gacaccggcg acctgacccc gagcatgaag ctgcgccgca
    14281 aggcggtcga ggccaaatac ctcaccgtcc tcgacgcgat gtacgagaac tgagccccgg
    14341 ctgctacaac ggcagcgtgc cctcgaccct gcccgacggt gagcccgccc ccgacgacgg
    14401 ccggctgccc gcggccgccc tggccggcct cggccaagcg ccgttcgggg tctacctgca
    14461 cgtgccgttc tgcaccaccc ggtgcggcta ctgcgacttc aacacctaca cggccgccga
    14521 gctggtcgcc cccgacggca ccacggtcag ccccacgtcg tacgtcgaga ccgcgctggc
    14581 cgagctgcgc ctggcccgca ccgtgctcgg cgacgcggtg ccaccggcga cgaccgtgtt
    14641 cgtcggtggg ggcaccccga cgctgctgcc ggcgaccgac ctcggccggc tgctcgacgg
    14701 cgtacggcgg gagctagggc tggccgccga tgtcgaggtg accaccgagg ccaaccccga
    14761 gtcggtcgac gcggcctacc tggccaccct gcgcgaggtc gggttcaccc gggtgtcgtt
    14821 cgggatgcag agcgcccgcc cgcacgtgct ggccgtgctc gagcgtcggc acaccccggg
    14881 tcggcccgag cagtgcgtgg cctgggcgag ggcggccggg ttcgagcacg tcaacctcga
    14941 cctgatctac ggaacgccgg gggagagcga cgacgactgg cgggcctcgg tgcaggccgc
    15001 gatcggcgcc ggccccgacc acgtgtcggc ctactcgctg atcgtcgagc cgggcacccg
    15061 gctggccgcg caggtgcgcc ggggccagct ggcgatgccc gacgacgacg tgctggccga
    15121 ccgctacctg atcgccgacg agctgctcag cgcggccggg ctgcactggt acgaggtgtc
    15181 caactgggcc acctcgcccg ccgcccgctc gcggcacaac ctgctctact ggaccggcgc
    15241 cgactggtgg ggggtcggac cgggcgcgca ctcgcacgtc ggcggcaccc ggtggtggaa
    15301 cgtcaagcac cccgcggccc atgccgcccg tctcgccgcg ggtcgcagcc cggcgcaggc
    15361 ccgcgaggtg ctttccgagg aggaccgcta cgtcgagcga gtgctgctcg gctcgcggct
    15421 gtccgacggg ctggcgctgg ccgagctgcg cgagccgggg cggacggcgg cggcgcaggc
    15481 ccgcgacgac gggctgctcg agcgttcgtc gtacgacgag ggccgtgcgg tgctgacgct
    15541 gcgggggcgg ctgctcggcg acgccgtcgt ccgcgacctg ctcgactgac cggcggcgcc
    15601 tcgcgggtca ggtgtcgagg ggatcgccgg tgacgaagtc gacgagctcc tcgacccggc
    15661 cgagcagggc gggctcgaga tcggtgtagt cg
//
LOCUS       DATIBB010000173        12545 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_237921, whole genome shotgun sequence.
ACCESSION   DATIBB010000173 DATIBB010000000
VERSION     DATIBB010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 12545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12545
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_237921"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(122..907)
                     /locus_tag="VLC50_06745"
     CDS             complement(122..907)
                     /locus_tag="VLC50_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013735542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HSF27203.1"
                     /translation="MPRTPGDPVYAAVIRAALAVFRALDLRLDLRGLDNVPASGGAVI
                     TINHTGYLDFAIAGLPFWRARRRLVRFMAKQQVFAHPVSGPLMRAMKHIPVDREAGSA
                     SFRAALTYLKRGELVGVFPEATISRSFCLKEFKLGAARMAMAAKVPVVPVVLWGSQRL
                     WTKGRARRLLRSRHVPVTVIVGPPLTAAAGEGAPAFTARIVAAMQAMLVAAQADYPQT
                     PRDDQDRWWLPAHLGGLAPTPETAAALDAAEADARASRTSAEA"
     gene            complement(965..3781)
                     /locus_tag="VLC50_06750"
     CDS             complement(965..3781)
                     /locus_tag="VLC50_06750"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HSF27204.1"
                     /translation="MSIDSFGARGTLEQGGKSYEIFRLSAVDGAEKLPYSLKVLLENL
                     LRTEDGHNVTAEHVRALAAWDPHAEPDTEIQFTPGRVIMQDFTGVPCVVDLAVMREAM
                     RDLGGDPAKVNPLAPSELVIDHSVIADVFGTPDSFERNVEIEYQRNRERYQFLRWGQS
                     AFDEFKVVPPGTGIVHQVNIEYLARVVMTRTVDGVLRAYPDTLVGTDSHTTMVNGLGV
                     LGWGVGGIEAEAAMLGQPVSMLIPQVVGFKLTNALPEGATATDLVLTITEMLRQTGVV
                     AKFVEFYGEGLAAVPLANRATIGNMSPEYGSTCAIFPIDDETIRYLRFTGRDEAQVAL
                     VEAYAKEQGLWNDPSVERVYSQSLELDLSTVVPSIAGPKRPQDRVKLSEAKEGFRSAL
                     ANYVEGTPQPLSQVDEGIEETFPASDPVAVVDDANGAAGAPRPAISAARGAEGRVSRP
                     TPVTLADGTQVEIDHGVVVIAAITSCTNTSNPQVMLGAALLAKKAVERGLRSKPWVKT
                     TLAPGSKVVIDYYERAGVLPYLEKLGFNLVGYGCTTCIGNSGPIIEEVSAAVNEADLA
                     AAAVLSGNRNFEGRINPDVKMNYLASPPLVVAYALAGSLDVDIMTEPLGQDPDGNDVF
                     LRDIWPTSQEIEELIEGSIASEMFSHDYGDVFAGDQRWQSLPTPEGDFFDWQEESTYV
                     RRPPYFEGMGATPDPVADIAGARVLAKLGDSVTTDHISPAGSIKADSPAGRYLVEHGI
                     ERKHFNSYGSRRGNHEVMIRGTFANIRLRNQLAPGTEGGFTRDFTRDGGPVTTIYDAA
                     MAYQAAGVPLVVLAGKEYGSGSSRDWAAKGTALLGVSAVIAQSYERIHRSNLIGMGVL
                     PLQFADGDSTDSLGLTGEETFAVSGVTALNDGLPRTVSVSATAPDGTVTRFEARVRID
                     TPAEADYYRHGGILQYVLRSLAAS"
     gene            complement(3841..5088)
                     /locus_tag="VLC50_06755"
     CDS             complement(3841..5088)
                     /locus_tag="VLC50_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012921370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HSF27205.1"
                     /translation="MGTHLELEVGPVAHGGHCVARHEGRVVFVRHALPGELVLAVVTD
                     GGASSRFLRADAIEVRRASPDRVEPPCPFAGPGRCGGCDWQHASLAAQRRLKADVVRE
                     ALRRQAGLDVPVVVEAVPGDLDGLGWRTRVHFAVDAHGRAGLRGHRSHEVVPVDDCRI
                     ASAGVRAPDALAATWPRARGLDVVSSATGQRAVVVHEASGRTRRAEGPGTVTELVGGR
                     PFRVGVDGFWQVHPGAARTLTEAVLDGLGPRPGESALDLYCGVGLFARALGDAVGPGG
                     SVTAVESDARAVSHARRNLHDAPHVRLVGDRVEKALSTGPVPLRADLVVLDPPRSGAG
                     ARVVAAVADRAPRRVGYVACDPVALARDLATFAGLGYGLRSLRAYDLFPMTAHVEVVA
                     ILGPADPVTSSGADRQPGILTSR"
     gene            complement(5100..7112)
                     /locus_tag="VLC50_06760"
     CDS             complement(5100..7112)
                     /locus_tag="VLC50_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020575717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HSF27206.1"
                     /translation="MRSDRLGHTLLPKRIALPVFASDALSSVAYAPDEILLTLSLAGL
                     TAYAYSWKIGLVVALVMLTVVASYRQNVHAYPSGGGDYEVATVNLGPRAGLVVASALL
                     VDYVLTVAVSISSGVQNASSALPFLKGHQVSAAVGLVLLLMAMNLRGVRESGTAFAIP
                     TYCFMAAILGMGLWGLFRYADGSLPMAESAGWELTPAPAYSEGLMGLAGAFLVLRAFS
                     SGCAALTGVEAISNGVPAFRKPKSKNAATTLLMMGTIAVTMLISIIVLARATGVQLAD
                     ETAGSMLLRDGVPVPPAEQHTVIGQLGQAVFSNFPPAFYFTAAATGIVLVLAANTAFN
                     GFPVLGSILGRDGYLPRQLHTRGDRLAFSNGIMMLAALAIVLIVAFDAQVTRLIQLYI
                     VGVFVSFTVSQTGMVRHWTRLLATESDPAVRRRMMRSRAINAFGLTLTGIVLVVVLVT
                     KFLAGAWIAILAMTLIFVLMRAIHSHYEAVARELEDTADDAPITLPSRNHAVVLVSKI
                     HKPTLRALAYARAAHPSSLEAVTVGVDDEEARALQDEWDRREIPVPLKVLDSPYREVT
                     RPVVDYVRGLRRSSPRDVVTVYIPEYVVGRWWEHLLHNQTALRLKGRLLFTPGVMVAS
                     VPWQLRSSQGLAERAEWPAPGSVRRGEPDLGASGQARRDPGAGGQP"
     gene            7302..7970
                     /locus_tag="VLC50_06765"
     CDS             7302..7970
                     /locus_tag="VLC50_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019072970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HSF27207.1"
                     /translation="MHVVIMGCGRVGSTLAHNLDDAGHSIAVIDQNKDAFRRLGPHFG
                     GRRLVGIGFDRETLAEAGIESADAFAAVSSGDNSNILAARVARETFGVQNVVARIYDP
                     GRAEVYQRLGIPTVATVRWTADQILRRLVPHGTEPVWRDPSGTIRLAESTYATSWVGE
                     RVSRLERETGARVAFLTRLGEGLLPDADTLVQDGDLMHLAIREDDVEQVERVLTRGPE
                     ESDH"
     gene            7970..8635
                     /locus_tag="VLC50_06770"
     CDS             7970..8635
                     /locus_tag="VLC50_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020542866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HSF27208.1"
                     /translation="MRIAIAGAGSVGRSIAKELLEHGHEVLLVDKEPGAIKVESVPKA
                     EWLLADACEIASLEEAGLERCDVVVAATGDDKVNLVVSLLAKTEYAVPRTVARVNNPK
                     NEWMFNEAWGVDVAVSTPRLMTALVEEAVSIGDLVQIFTFQQSNTDLLELTLPATSPM
                     VGTRVADVQWPAETVLVAIIREGQPLVPGPDDPLESGDQLLFVAAPESGAALSELLAP
                     HAG"
     gene            complement(8660..9298)
                     /locus_tag="VLC50_06775"
     CDS             complement(8660..9298)
                     /locus_tag="VLC50_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007077917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3159 domain-containing protein"
                     /protein_id="HSF27209.1"
                     /translation="MTPTGPDLDTVEAVVRAQLAKALGGRRGMVEAAVPTVVFTLTWI
                     LREDLPLALWLGAGSAAVLLVVRLATRQTVQFVLNSLVGIGIAAVFALRSGRAEDAFL
                     PGILYNGGYAVVLILSVVLRWPLVGFLVGAVTGDPSAWRSNPALVRLTSRLTLLLALP
                     CVLRVLVQYPLYAAGEVGWLGVAKITLGWPLQVAALLAMAWLLARNRTPLTS"
     gene            complement(9295..9678)
                     /locus_tag="VLC50_06780"
     CDS             complement(9295..9678)
                     /locus_tag="VLC50_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006602616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HSF27210.1"
                     /translation="MSEQTTGRLRRVLSRWVSSTQELEAEELHEDSLERGCTAVEALV
                     PRGRVRVSGTLRSVTLRPRAGVPALVADLYDGTGILTVVWLGRRRIPGIVPGRMLVAS
                     GRVLVEDGAPTIYNPRYQLLPGVPE"
     gene            complement(9700..10482)
                     /locus_tag="VLC50_06785"
     CDS             complement(9700..10482)
                     /locus_tag="VLC50_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3710 domain-containing protein"
                     /protein_id="HSF27211.1"
                     /translation="MFRRRRNDEATARQDGSDTELDEVEADTEAAPVHAELDRSSGPW
                     DVTEVEADEPRLDLGSVRLPAAAGMEVQLQLDEASGQVVAVLAVIGQTALQLQAFAAP
                     RTEGIWDEVRAEIAAGLTGQGGTCSEAEGPFGAELRAQVPATLPDGTAGFQQVRFLGV
                     DGPRWFLRGVVSGAGAVDAAAAAPLEDVFRRTVVVRGDVPMAPRTPIPLHAPQAAAPE
                     ATAEQTPESESAESAETTVPKGRRGREALKPFERGPEITEIR"
     gene            complement(10463..10996)
                     /gene="dut"
                     /locus_tag="VLC50_06790"
     CDS             complement(10463..10996)
                     /gene="dut"
                     /locus_tag="VLC50_06790"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018656801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HSF27212.1"
                     /translation="MPTHPDHAEHVDVLLRRLDPGVPVPSCAHPGDAGADLVARVDVE
                     LAPGERALVPTGVAIALPTGWAAFVHPRSGLAARHGVTVVNAPGTVDAGYRGEILVNL
                     LNTDRTQTVRLRRGDRIAQLVVQRVGLARFHEVDLLPGSVRGEGGHGSTGGLTPDTPR
                     PARPSADEGDNRVPTTP"
     gene            11084..11602
                     /locus_tag="VLC50_06795"
     CDS             11084..11602
                     /locus_tag="VLC50_06795"
                     /inference="COORDINATES: protein motif:HMM:NF022736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3093 domain-containing protein"
                     /protein_id="HSF27213.1"
                     /translation="MPADQPTGSGRLLYAERLWAPVMLWVVVIAMVASLAVAYGAAMG
                     TAGGLVTGLLAGAVAAVFIIRSAALVQVTEREVVAGRARIPVEAIGPPVALDAERTRW
                     LRGQGIDPAAYHLIRGWVPTSVHVPVVDPRDRTPYWLIATRHPDRLAAAIEAAQAAGG
                     QPGSAGTEPDRG"
     gene            complement(11674..11973)
                     /locus_tag="VLC50_06800"
     CDS             complement(11674..11973)
                     /locus_tag="VLC50_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014145338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4193 domain-containing protein"
                     /protein_id="HSF27214.1"
                     /translation="MATDYDSPRKTDDDVDQDSIEELKARRNEKSSGSVDIDEAEQAE
                     GFELPGADLSNEELSVRVLPRQADEFTCSRCFLVHHRSQLAKADGTQLICKECAA"
     gene            complement(12109..>12545)
                     /locus_tag="VLC50_06805"
     CDS             complement(12109..>12545)
                     /locus_tag="VLC50_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017618181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HSF27215.1"
                     /translation="TWSATLDGPGTCDGEPVHASTRTELAKALVATGFGYAASRRARQ
                     AEVLREVLPSVRDIRRAGAASLDLCAVAAGQVDAYYEQGLKPWDHAAGALVARRAGAL
                     VGGLHGRPAGERLVVAGPPVLFEALVGLLERLRADEDVPLDT"
BASE COUNT         1769 a         4498 c         4630 g         1648 t
ORIGIN      
        1 tcgtagcccc ggtagtcggc ggggcacatg tccagcagcc atccgaccgc gctgcgctcc
       61 cagtcggggg aaccgggtgg gttgacttcg ctgggccagc ccggcgggcg gaccggggcg
      121 ttcatgcctc ggcggaggtg cgcgaggctc gggcgtcggc ctcggccgcg tcgagcgcgg
      181 cggcggtctc aggggtgggc gcgagcccgc ccaggtgtgc aggcagccac caccggtctt
      241 ggtcatcacg cggggtctgc gggtagtcgg cctgggctgc cacgagcatg gcctgcatcg
      301 cggcgacgat gcgcgcggtg aacgcgggcg caccctcgcc tgcggccgcc gtcaggggcg
      361 gcccgacgat gacggtgacc ggcacgtgcc gggagcggag cagccgacgg gcgcggccct
      421 tggtccacag ccgctggctg ccccacagca cgacgggcac caccggcacc ttggccgcca
      481 tcgccatgcg ggccgcgccg agcttgaact ccttgaggca gaagctgcgg ctgatggtgg
      541 cctcggggaa gacccccacc agctcgccgc gcttgaggta ggtcagcgcc gcccggaagg
      601 acgccgagcc ggcctcccgg tcgaccggga tgtgcttcat cgcccgcatg agcgggccgg
      661 acaccgggtg ggcgaagacc tgctgcttgg ccatgaaccg gaccagccgc cgccgggccc
      721 gccagaacgg caggccggcg atcgcgaagt cgaggtatcc ggtgtggttg atcgtgatga
      781 ccgcgccgcc ggaggccggc acgttgtcca ggccccgcag gtcgagcctc aggtcgagcg
      841 cccggaagac cgccagcgcg gcgcggatga cggcggcgta gaccggatca ccgggggtcc
      901 gtggcatgag gctcacgcta ccggtcgcca cggacgacgc cgcggcgacc gggagcgcga
      961 atcgtcagct ggccgccagc gaccgcagga cgtactgcag gatcccgccg tgccggtagt
     1021 agtcggcctc ggcgggggtg tcgatgcgca cccgcgcctc gaaccgggtg acagtgccgt
     1081 cgggcgcggt ggcgctcacc gacaccgtgc ggggcaggcc atcgttgagc gcggtgaccc
     1141 cgctgaccgc gaaggtctcc tcaccggtca ggccgagcga gtcggtgctg tccccgtcgg
     1201 cgaactgcag cggcagcacg cccatcccga tgaggttgga ccggtggatg cgctcgtagg
     1261 actgggcgat gaccgcgctg acgccgagca gcgcggtgcc cttggcggcc cagtcgcgcg
     1321 aggaccccga gccgtactcc ttgccggcca gcacgaccag cggcaccccg gcggcctggt
     1381 aggccatcgc ggcgtcgtag atcgtcgtga ccggaccgcc gtcgcgggtg aagtcacggg
     1441 tgaacccgcc ctcggtcccg ggggcgagct ggttgcgcag ccggatgttg gcgaacgtgc
     1501 cgcggatcat cacctcgtgg ttgccgcgac gcgagccgta ggagttgaag tgcttgcgct
     1561 cgatgccgtg ctcgaccagg tagcgaccgg ccgggctgtc ggccttgatc gagccggccg
     1621 ggctgatgtg gtcggtggtc accgagtcgc cgagcttggc cagcacccgg gccccggcga
     1681 tgtcggcgac cgggtcgggc gttgcgccca tgccctcgaa gtagggcggc cggcgcacgt
     1741 aggtggactc ctcctgccag tcgaagaagt cgccctcggg ggtcggcagc gactgccacc
     1801 gctggtcgcc ggcgaacacg tcgccgtagt cgtgggagaa catctccgag gcgatcgagc
     1861 cctcgatgag ctcctcgatc tcctggctgg tcggccagat gtcgcgcagg aagacgtcgt
     1921 tgccgtccgg gtcctggccc agcggctcgg tcatgatgtc gacgtcgagc gagccggcga
     1981 gcgcgtaggc caccaccagc ggcggggagg ccaggtagtt catcttgacg tcggggttga
     2041 tccggccctc gaagttgcgg ttgcccgaga gcaccgcggc cgcggccagg tcggcctcgt
     2101 tgaccgccgc gctgacctcc tcgatgatcg gcccagagtt gccgatgcag gtggtgcagc
     2161 cgtagccgac caggttgaac ccgagcttct cgaggtaggg cagcaccccg gcccgctcgt
     2221 agtagtcgat gaccaccttg gaccccgggg cgagggtggt cttgacccag ggcttgctgc
     2281 gcaggccgcg ctcgaccgcc ttcttggcga gcagggcggc gccgagcatg acctgcgggt
     2341 tggacgtgtt ggtgcacgac gtgatggctg cgatcaccac gacgccgtgg tcgatctcga
     2401 cctgcgtgcc gtcggccagc gtcaccgggg tgggccggct gacccgaccc tcggcgccac
     2461 gcgcggcgga gatggccggg cgcggcgcgc ccgcggcacc gttggcgtca tcgaccaccg
     2521 cgaccgggtc ggaggccggg aaggtctcct cgatgccctc gtccacctgg gagagcggct
     2581 gcggggtgcc ctcgacgtag ttggccagcg ccgaccggaa gccctccttg gcctcggaca
     2641 gcttgacccg gtcctgcggg cgcttggggc cggcgatgct cggtacgacg gtcgacaggt
     2701 cgagctcaag ggactgggag tagacgcgct cgacgctggg gtcgttccac agcccctgct
     2761 ccttggcgta agcctcgacc agcgcgacct gcgcctcgtc gcggccggtg aagcgcaggt
     2821 agcggatcgt ctcgtcgtcg atcgggaaga tcgcgcaggt cgagccgtac tcggggctca
     2881 tgttgccgat ggtggcccgg ttggccagcg gtacggcggc caggccctcg ccgtagaact
     2941 cgacgaactt ggcgaccacg ccggtctggc gcagcatctc ggtgatcgtg aggaccaggt
     3001 cggtcgcggt ggcgccctcg ggcagcgcgt tggtcagctt gaagccaacc acctgcggga
     3061 tgagcatcga gaccggctgg ccgagcatgg ccgcctcggc ctcgatgccg ccgacccccc
     3121 agccgagcac gccgaggccg ttgaccatcg tggtgtgcga gtcggtgccg accagcgtgt
     3181 cggggtaggc gcgcagcacg ccgtcgacgg tgcgggtcat cacgacccgg gccaggtact
     3241 cgatgttgac ctggtggacg atgccggtgc ccgggggcac gaccttgaac tcgtcgaacg
     3301 cgctctggcc ccagcgcagg aactggtagc gctcgcggtt gcgctggtac tcgatctcga
     3361 cgttgcgctc gaacgagtcg ggggtgccga acacgtcggc gatgacggag tggtcgatga
     3421 ccagctcgga cggagccaac gggttgacct tggccggatc accaccgagg tcgcgcatcg
     3481 cctcacgcat caccgcaagg tcgaccacgc acgggacgcc ggtgaagtcc tgcatgatca
     3541 cgcggccggg ggtgaactgg atctcggtgt ccggctcggc gtgcgggtcc caggcggcca
     3601 gggcgcgcac gtgctctgcg gtgacgttgt gcccgtcctc ggtgcgcagg aggttctcga
     3661 gcagcacctt gaggctgtag ggcagcttct cggcgccgtc aacggccgac aggcggaaga
     3721 tctcgtagct cttcccgccc tgctcaagcg ttcccctggc accgaagctg tcgatgctca
     3781 tgtgcttctc ctctcctgcc accggccatc ctgccgcacg gccccacggc tcgtcgcagg
     3841 ttatctcgat gtcaagatac caggctgtcg atctgctcca gacgacgtga ccgggtcggc
     3901 gggccccagg atcgccacga cctccacgtg cgcggtcatg gggaagaggt cataggcccg
     3961 cagcgagcgc aacccgtagc ccaggccggc gaaggtggcc aggtcgcgcg cgagcgcgac
     4021 cgggtcgcag gcgacgtagc cgacccgccg tggcgcccgg tcggcgaccg cagcgaccac
     4081 ccgggcgccg gcgccggagc gtggggggtc gaggaccacg aggtcggccc ggagcggcac
     4141 cggcccggtg gacagtgcct tctcgacccg gtcgccgacc agccgaacgt gcggcgcgtc
     4201 gtgcaggttg cgccgggcgt gcgagacggc gcgggcgtcg gactcgaccg cggtgaccga
     4261 cccgccgggc ccgacagcgt caccgagcgc gcgcgcgaac agcccgaccc cgcagtagag
     4321 gtcgagcgcc gactcccccg gtcgcgggcc cagcccgtcg agcacggcct cggtcagggt
     4381 ccgggccgct cctgggtgca cctgccagaa cccgtcgaca cccacccgaa agggccgccc
     4441 cccgacgagc tcggtcacgg tgccggggcc ctcggcgcgg cgggtccggc cgctcgcctc
     4501 gtgcaccacc accgcacgct gacccgtggc ggagctgacc acgtcgagcc cacgggctcg
     4561 cggccaggtc gccgccaggg cgtccggcgc tcgtacgccg gcactggcga tgcggcagtc
     4621 gtcgaccggc acgacctcgt gggagcggtg cccgcgcaga ccggcccggc cgtgggcgtc
     4681 gaccgcgaag tgcacccgag tgcgccagcc cagcccgtcg aggtcgccgg gcaccgcctc
     4741 gacgacgacc gggacgtcga ggccggcctg ccggcgcagc gcctcgcgca cgacgtcggc
     4801 cttgagccgt cgctgggcgg ccagcgaggc gtgctgccag tcgcacccgc cgcagcgccc
     4861 cggcccggcg aacgggcacg gcggctcgac ccggtcggga gaggcccggc gcacctcgat
     4921 cgcgtcggcg cgcaggaacc gcgagctcgc accgccgtcg gtgaccacgg cgaggaccag
     4981 ctcgccgggc agggcgtggc gcacgaagac gacccggccc tcgtgccggg ccacgcagtg
     5041 gccgccgtgc gccaccgggc cgacctcgag ctcgaggtgg gtgcccacca ggtcggtgct
     5101 cagggctggc cccctgcccc ggggtcgcgc cgggcctgcc cgctcgcgcc caggtccggc
     5161 tcgccgcggc gcaccgagcc cggtgccggc cactcggccc gctcggcgag cccctgcgag
     5221 gagcgcagct gccagggcac gctggccacc atcactccgg gcgtgaacag cagccgaccc
     5281 ttcagccgca gcgcggtctg gttgtgcagc aggtgctccc accagcgacc gacgacgtac
     5341 tcggggatgt agaccgtgac gacatcgcgt gggctgctgc gccgcaggcc gcgcacatag
     5401 tcgacgaccg gtcgggtgac ctcgcggtag ggcgagtcga gcaccttcag cgggacgggg
     5461 atctcgcggc ggtcccactc gtcctgcagt gcccgggcct cctcgtcgtc caccccgacg
     5521 gtgaccgcct cgagcgagga cgggtgggcc gcccgggcgt aggccagcgc ccgcagggtg
     5581 ggcttgtgga tcttggagac gagcacgacg gcgtggttgc gcgagggcag cgtgatgggc
     5641 gcgtcgtcgg cggtgtcctc gagctcacgg gccacggcct cgtagtggct gtggatggcg
     5701 cgcatcagca cgaagatcag cgtcatggcg aggatggcga tccaggcgcc ggccaggaac
     5761 ttcgtgacga gtacgacgac cagcacgatg ccggtgagcg tgagcccgaa cgcgttgatc
     5821 gcccgcgagc gcatcatccg gcgccgtacg gccgggtcgg actcggtcgc gagcaggcgg
     5881 gtccagtgcc gcaccatgcc ggtctggctc acggtgaacg agacgaacac gccgacgatg
     5941 tagagctgga tcagccgggt cacctgggcg tcgaacgcga cgatcagcac gatggccagc
     6001 gccgccagca tcatgatgcc gttgctgaac gcgagccggt cgccgcgggt gtgcagctgg
     6061 cgtggcaggt agccgtcgcg gcccaggatc gagccgagca ccgggaaccc gttgaacgcg
     6121 gtgttggccg cgagcacgag gacgatgccg gtcgcggcgg cggtgaagta gaaggccggc
     6181 gggaagttgg agaagaccgc ctggccgagc tggccgatca cggtgtgctg ctcggccggc
     6241 ggcaccggca cgccgtcccg cagcagcatc gagcccgccg tctcgtcggc cagctgcacg
     6301 ccggtggccc gggccagcac gatgatgctg atgagcatgg tcaccgcgat cgtgcccatc
     6361 atcagcagcg tggtggcggc gttcttggac ttgggcttgc ggaaggccgg cacgccgttg
     6421 ctgatcgcct cgaccccggt cagcgcagcg catccagacg agaaggcccg caggacgagg
     6481 aacgcccccg ccagccccat cagcccctcg ctgtaggccg gggcgggggt cagctcccag
     6541 ccggcgctct cggccatcgg cagcgacccg tcggcgtaac ggaacagtcc ccacagcccc
     6601 atcccgagga tcgcggccat gaagcagtag gtcgggatgg cgaacgccgt gcccgactcc
     6661 cgcaccccac gcaggttcat cgccatgagc agcagcacca gcccgaccgc cgcggagacc
     6721 tggtggccct tgaggaaggg cagtgccgag gaggcgttct gcacgcccga ggagatcgag
     6781 acagccaccg tgaggacgta gtcgacgagc agcgcgctgg ccacgaccag gccagcgcgc
     6841 ggaccgaggt tgacggtggc cacctcgtag tcgccgccgc cgctgggata ggcgtgcacg
     6901 ttctgccggt aggacgcgac gaccgtcagc atcaccagcg cgacgaccag gccgatcttc
     6961 cagctgtagg cgtaggccgt cagcccggcc agggagagcg tgagcaggat ctcgtcgggc
     7021 gcgtaggcca cggaggacag cgcgtcgctg gcgaacacgg gcagcgcgat ccgcttaggg
     7081 agcagcgtgt gaccgagtcg atcgctgcgc atcgcccggc cgaggaagac ccgcttggcc
     7141 agatccgtgg aacgcggcac gtcggcacat gctaggcccc gccgacccgc ccggcgccgg
     7201 gccgccacgt cgtcctagcc gtgtagcgtt cccccggtgc cggtcggcag aggacgccgg
     7261 cgggagcgag gcgacgggat cggccgggaa gtgagctcag cgtgcatgtc gtgatcatgg
     7321 gctgcggtcg tgtcggctcg acgctggcgc acaacctcga cgacgccggg cacagcatcg
     7381 cggtgatcga ccagaacaag gacgcgttcc ggcggctggg cccgcacttc ggtgggcgcc
     7441 gcctcgtcgg gatcgggttc gaccgcgaga cgttggccga ggcgggcatc gagtcggccg
     7501 acgcgttcgc ggcggtctcc agcggtgaca actccaacat cctcgcggcc cgggtggccc
     7561 gggagacgtt cggcgtgcaa aacgtcgtgg cccgcatcta cgaccccggc cgggccgagg
     7621 tctaccaacg gctgggcatc cccacggtgg cgaccgtgcg gtggaccgcc gaccagatcc
     7681 tgcgccggct ggtcccgcac gggaccgagc cggtctggcg cgacccgtcc ggcacgatcc
     7741 ggttggccga gagcacctac gccacctcct gggtcggcga gcgggtcagc cggctcgagc
     7801 gggagaccgg cgcccgggtc gcgttcctga cccggctggg cgaggggctg ctgccggacg
     7861 ccgacaccct cgtgcaggac ggcgacctca tgcacctggc catccgcgag gacgacgtcg
     7921 agcaggtcga acgggtcctc acgcgcggcc ccgaggagag cgaccactga tgcgcatcgc
     7981 catcgccgga gccggcagcg tgggccggtc gatcgccaag gagctgctcg agcacggcca
     8041 cgaggtcctg ctcgtcgaca aggaacccgg cgcgatcaag gtcgagtcgg tacccaaggc
     8101 cgagtggctg ctggccgacg cctgcgagat cgcctcgctc gaggaggcag ggctcgagcg
     8161 ctgcgacgtc gtggtggccg ccaccggcga cgacaaggtc aacctcgtcg tctcgctgct
     8221 ggccaagacc gagtacgccg tcccgcgcac cgtcgcccgg gtcaacaacc ccaagaacga
     8281 gtggatgttc aacgaggcgt ggggggtcga cgtcgcggtc tccacgcccc ggctgatgac
     8341 cgcgctcgtc gaggaggccg tcagcatcgg cgacctcgtg cagatcttca ccttccagca
     8401 gtccaacacc gacctgctcg agctcacgct gccggcgacc tcgccgatgg tgggcacccg
     8461 ggtcgccgac gtgcagtggc cggccgagac cgtgctcgtg gccatcatcc gcgagggtca
     8521 gccgctcgtt ccaggccccg acgacccgct ggagtccggc gaccagctgc tcttcgtggc
     8581 cgccccggag tcgggcgcgg cgctgagcga gctgctcgcc ccgcacgcgg gctgacggtc
     8641 tgaggcagct cagccgggct cagctcgtca agggagtgcg gttgcgtgcc agcagccagg
     8701 ccatcgcgag cagcgccgcc acctgcagcg gccagcccag ggtgatcttg gcgacgccca
     8761 gccacccgac ctcgccggcg gcgtagagcg ggtactgcac caggacacgc agcacgcacg
     8821 gcagcgcgag cagcagggtc agccgcgacg tcagccggac caaggcgggg ttggaccgcc
     8881 aagcggacgg gtcgccggtc accgcgccca ccaggaagcc gaccagcggc catcgcagga
     8941 cgaccgagag gatcagtacg acggcatagc cgccgttgta gaggatgccc ggcaggaagg
     9001 cgtcctccgc tcgtccggac cgcagggcga agaccgccgc gatcccgatg ccgaccaggc
     9061 tgttgagcac gaactgcacg gtctggcgcg tggccagccg cacgacgagc aggaccgcgg
     9121 ccgaaccggc accgagccag agcgccagcg gaaggtcctc gcgcaggatc caggtgaggg
     9181 tgaacaccac ggtcggcacg gccgcctcga ccatcccgcg gcgcccaccc agggctttgg
     9241 ccagctgggc gcgcaccacg gcctcgaccg tgtccaggtc gggcccggtg ggggtcactc
     9301 gggcacgccc ggcaacagct ggtagcgcgg gttgtagatc gtcggcgcgc cgtcctcgac
     9361 cagcacccgg cccgaggcca ccagcatccg cccgggcacg atcccgggga tccgccgccg
     9421 tccgagccac accaccgtca ggatcccggt gccgtcatag aggtcggcga cgagcgccgg
     9481 caccccggcc cgcggccgca gcgtcaccga gcgcagggtg ccagacaccc gcacccgccc
     9541 ccggggcacc agcgcctcga ccgcggtgca gccgcgctcg aggctgtcct cgtgcagctc
     9601 ctcggcctcg agctcctggg tggaggagac ccaccgggag agcacccggc gcagccgacc
     9661 ggtcgtctgc tcgctcatcg tcgtcacctg cctcggcggt cagcggatct cggtgatctc
     9721 cggaccgcgc tcgaacggct tgagcgcctc gcggccgcgc cggcccttgg gcacggtcgt
     9781 ctccgccgac tccgccgact ccgactctgg cgtctgctct gccgtggcct ccggcgccgc
     9841 ggcctgcggg gcgtgcagcg ggatcggtgt gcgcggggcc atcggcacgt caccgcgcac
     9901 gacgacggtg cggcggaaga cgtcctccag gggcgccgcc gcggccgcgt cgaccgcccc
     9961 cgcgccgctg acgaccccgc ggaggaacca gcgaggcccg tccacgccga ggaagcgcac
    10021 ctgctgaaaa ccggccgtcc cgtcggggag cgtggccggg acctgggcgc gcagctcggc
    10081 cccgaagggc ccctcggcct cggagcaggt gccgccctgc ccggtcagcc ccgccgcgat
    10141 ctcggcgcgg acctcgtccc agatcccctc ggtgcgcggg gcggcgaacg cctgcagctg
    10201 cagcgcggtc tgcccgatca cggcgagtac ggcgaccacc tgcccggacg cctcgtcgag
    10261 ctggagctgg acctccatgc ctgcggcggc cggcaggcgc accgagccca ggtcgagccg
    10321 cggctcgtcg gcctcgacct cggtgacgtc ccaggggccg ctggagcggt cgagctcggc
    10381 gtggaccggc gcggcctcgg tgtcggcctc gacctcgtcg agctcggtgt cgctgccgtc
    10441 ctgacgtgcg gtcgcctcgt cgttacggcg tcgtcggaac acggttgtct ccttcatcgg
    10501 cgctcggccg ggctggacga ggtgtgtcgg gggtcagccc gccggtagac ccgtgcccac
    10561 cctcgccccg gaccgaaccc ggcagcaagt cgacctcgtg gaagcgagcc agcccgaccc
    10621 gttggacgac gagctgagcg atccggtcgc cacgacgcag ccgcaccgtc tgggtgcggt
    10681 cggtgttgag caggttgacc aggatctcgc cccggtagcc ggcgtcgacc gtgccggggg
    10741 cgttgaccac cgtgacgccg tggcgagcgg ccagcccgga gcgggggtgc acgaaggccg
    10801 cccaccccgt cggcaacgcg atggccactc cggtcggcac caacgcgcgc tcaccggggg
    10861 cgagctcgac gtcgacccgg gcgaccaggt cggctccggc gtcaccgggg tgcgcacagc
    10921 tcggcaccgg cacccccggg tcgaggcggc gcagcagcac gtcgacgtgc tcggcgtggt
    10981 ccgggtgggt gggcacggag ggcgagacta ccggcgccgg ggtcgtcgtc cggccgcgac
    11041 gggcccggcc aaccgtcgat cgggcgggtg agaggctgac cccatgccag cggatcagcc
    11101 gaccgggtct ggacggctgc tctatgccga gcgcctgtgg gcccccgtca tgctgtgggt
    11161 ggtcgtcatc gcgatggtcg ccagcctcgc cgtggcctac ggcgcggcga tgggcacggc
    11221 aggtgggctg gtgaccggcc tgctggccgg ggcggtggcc gcggtcttca tcatccgctc
    11281 cgccgcgctc gtgcaggtga ccgagcgcga ggtggtcgcc ggccgggccc gcatcccggt
    11341 cgaggccatc gggccgccgg tggcgctgga tgccgagcgg acccgttggc tgcgcgggca
    11401 gggcatcgac ccggccgcct accacctgat ccgcggatgg gtccccacgt cggtccatgt
    11461 gccggtcgtc gacccgcgcg accggacgcc gtactggttg atcgccaccc gtcatcccga
    11521 ccgcttggcg gcggcgatcg aggccgccca ggccgccggc gggcagcccg gctccgccgg
    11581 caccgagccc gatcgcggct gagacgcacg gccgcccggc cacaggccgg gcggcgacgt
    11641 cggagtcggg agatcgaacg cggcggatcg cactcaggcg gcgcactcct tgcagatcag
    11701 ctgggtgccg tcggccttgg ccagctggct gcggtggtgg accaggaagc agcgcgagca
    11761 ggtgaactcg tcggcctggc gcggcagcac ccggaccgac agctcctcgt tggacaggtc
    11821 ggcaccaggc agctcgaagc cctcggcctg ctcggcctcg tcgatgtcca ccgaccccga
    11881 ggacttctcg ttgcggcggg ccttcagctc ctcgatgctg tcctggtcga cgtcatcgtc
    11941 ggtcttgcgt gggctgtcgt aatctgtcgc catcgtcggt caccctcccg aggtgtgcac
    12001 ggctgctcgc gctactaacg cgccccggtc gggttttgtg cccggcggcg gccgggcgat
    12061 tctccctcat cgggtcggct gatcagccac cagggcgcgt gacccgcatc acgtgtcgag
    12121 cggcacgtcc tcgtcggccc gcaggcgctc gagcaggccc accagggcct cgaagaggac
    12181 gggcggacct gccacgacga gacgctcccc ggccggccgc ccgtgcaggc caccgacgag
    12241 cgcgccggcc cgccgggcga ccagcgcgcc ggccgcgtgg tcccacggct tgaggccctg
    12301 ctcgtagtag gcgtcgacct gcccagcggc caccgcgcag aggtccagcg aggccgcccc
    12361 ggcgcgccgg atgtcgcgca cgctcggcag cacctcgcgc agcacctcgg cctggcgggc
    12421 ccgacgggac gcggcatagc cgaacccggt ggccaccaga gccttggcga gctcggtgcg
    12481 ggtcgaggcg tgcaccggct cgccgtcgca ggtgcccggc ccgtcgaggg tcgccgacca
    12541 ggtgt
//
LOCUS       DATIBB010000174        10209 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_897554, whole genome shotgun sequence.
ACCESSION   DATIBB010000174 DATIBB010000000
VERSION     DATIBB010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 10209)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10209)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10209
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_897554"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..209)
                     /locus_tag="VLC50_06810"
     CDS             complement(<1..209)
                     /locus_tag="VLC50_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018834271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor family protein"
                     /protein_id="HSF27216.1"
                     /translation="MKTDVETLDPTRVRLTVEVPFDELKPSLDAAYKRIAGQVNVPGF
                     RKGKVPARIIDQRFGRAVVLEEAVN"
     gene            complement(305..380)
                     /locus_tag="VLC50_06815"
     tRNA            complement(305..380)
                     /locus_tag="VLC50_06815"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(345..347),aa:Pro,seq:tgg)
     gene            481..551
                     /locus_tag="VLC50_06820"
     tRNA            481..551
                     /locus_tag="VLC50_06820"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:513..515,aa:Gly,seq:tcc)
     gene            complement(621..1130)
                     /locus_tag="VLC50_06825"
     CDS             complement(621..1130)
                     /locus_tag="VLC50_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012228412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HSF27217.1"
                     /translation="MAKAEAVFIYIGTYPNEGAARGDYAVVKDLHDVGAIGTYDAAVI
                     TKDAEGKVHVNKDETATRRGAWGGAAVGAVVGILFPPSIIGSALVGAAVGGVGGHLWR
                     GMSRADIKEFGEIIDEGRAALVIVGESTLRQAVDKAGLKADKHVTKDLDVRPEDVDKA
                     VQEAAKDVS"
     gene            complement(1174..1797)
                     /locus_tag="VLC50_06830"
     CDS             complement(1174..1797)
                     /locus_tag="VLC50_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020016372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HSF27218.1"
                     /translation="MFVRTTEVQADPAKIDAGIAVVRDDIFPAASEIDGCVGMSLLVN
                     RDSGRCIATTAWRSQDAMRASAEQVVPLRDRAEQTLGASGSEVHQWEVAVVHRDRAAP
                     EGACARLTWLSGDPATADRAIDTYKMVVLPRLAAMDGFCSSSLMIDRDAGRVVGTVVF
                     ESRGALEATRDAAKGIRERVASELGATVDDVEEIEVAFAHLHVPEMA"
     gene            complement(1891..2460)
                     /locus_tag="VLC50_06835"
     CDS             complement(1891..2460)
                     /locus_tag="VLC50_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HSF27219.1"
                     /translation="MSATYTFDVFSSLDGYGGASANWTGYWGKQGPELLDHRLALYDA
                     EQRMVFGANTYRAFVQLLASEDPDVRDPWVTRMRNMPATVVSTTLQGPLDWPNANLVS
                     GDALDVVAELKEESDVPLRSHGSLSMNRALMAAGLVDFVQVTIFPVVTGQTGLDPIFR
                     GAADFDLELVESQTLDGRTQELVYRPTLH"
     gene            complement(2494..2910)
                     /locus_tag="VLC50_06840"
     CDS             complement(2494..2910)
                     /locus_tag="VLC50_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013285392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaB family protein"
                     /protein_id="HSF27220.1"
                     /translation="MPASKDMPSTLRRSSAKAQRTWAKTHDAAVDEYGEGERAHRTAF
                     AALKHSFEKVGDHWEEKPGGAKGPSDSQAARRGGPARRGGRTAGGVDTHASKSHLMEV
                     AKRLEIRGRSAMSKGELVDAIKSANESSTRRSRGGR"
     gene            complement(2938..3315)
                     /locus_tag="VLC50_06845"
     CDS             complement(2938..3315)
                     /locus_tag="VLC50_06845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF27221.1"
                     /translation="MIILGFLLIIISVAAAVILIVQNTTDVDVSALGQSWTVPALWLV
                     VAGLVTMAVAVIGLVLIRQARPRIPQHRVPREAQGTVPSQSPQGVSDREPAPDATRET
                     TPETGEAQQPSIPLSRNEPGGSS"
     gene            complement(3342..3515)
                     /locus_tag="VLC50_06850"
     CDS             complement(3342..3515)
                     /locus_tag="VLC50_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019969943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HSF27222.1"
                     /translation="MGAEDKLQNKADEMAGKAKEKVGEMTGNESMEKEGKKDETKANV
                     KQAGEKIKDVFKD"
     gene            3697..3852
                     /locus_tag="VLC50_06855"
     CDS             3697..3852
                     /locus_tag="VLC50_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015613097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6131 family protein"
                     /protein_id="HSF27223.1"
                     /translation="MIILGVILLIIGLVAGIGVLWTIGIILVVIGAVLWIAGATGHAV
                     GGRSHYW"
     gene            complement(3855..4583)
                     /locus_tag="VLC50_06860"
     CDS             complement(3855..4583)
                     /locus_tag="VLC50_06860"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF and ANTAR domain-containing protein"
                     /protein_id="HSF27224.1"
                     /translation="MSAPRLWDATAAGSHRLDETACAERFVRDVTITAASRLAPMSQV
                     AVTTIERGSVADRSSSDRRCVDCESVGHSSGQGPGVNALATDRIVLVRDLENDQRWPV
                     WADLARSHGFRSAAALPAQVVDGSIILLVGYSETGDAWDPSSLAAMARCAQELAHLLA
                     LSRRCAEQVQANAHLKSALASRGTIGQAMGIIMAQNRCSADTAFAILRSASQHRNLKL
                     REVAASLTEAVCDAPSDSSAFREG"
     gene            complement(5105..5359)
                     /locus_tag="VLC50_06865"
                     /pseudo
     CDS             complement(5105..5359)
                     /locus_tag="VLC50_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018784979.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="short-chain dehydrogenase"
     gene            complement(5356..5621)
                     /locus_tag="VLC50_06870"
                     /pseudo
     CDS             complement(5356..5621)
                     /locus_tag="VLC50_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014738595.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional heptose 7-phosphate kinase/heptose
                     1-phosphate adenyltransferase"
     gene            complement(6154..7692)
                     /locus_tag="VLC50_06875"
     CDS             complement(6154..7692)
                     /locus_tag="VLC50_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013226110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HSF27225.1"
                     /translation="MSLDYAVVVPTIGRASLTRLLTSLAGQPFDHEHPAPVEAVVVDD
                     RPLRAAAPPPLALPPLPCPTRVIRSGGRGPAAARNAGWRSVRTEWVVLLDDDVELPPQ
                     WVHELAADLDRAPSSVAGVQARLHVPVSADRPQSDWERNTAGLEQARWATADMAYRRR
                     ALAEVGGFDERFPRPYREDADLALRVRGQGWTLRRGHRITHHPVRPADFWVSVRTQVG
                     GADDALMRTLHGPRWRTRAQTGVGRARWHAATVAAGALAAGAGAVRRHRLATAGGLLW
                     LSLYADFLVRRLVDGPRPGQPGWVGEWARMTTTSAVIPPVALAHRVGGTLRHARAVPP
                     WPPPVRAVLLDRDGTLVQDVPYNGDPDRVVVVADARAVLDRLRAAGLAVGVVTNQSGL
                     ALGWLTPTQVRAVNARIMAELGPFDVWEQCPHGPDAGCPCRKPGPALVLRAAARLGIL
                     PAECLVIGDTGADVGAGVAAGARAVLVPAPLTRPDEVSAAKATVGVAATLTDAVAGLV
                     GVSR"
     gene            complement(7689..8605)
                     /locus_tag="VLC50_06880"
                     /pseudo
     CDS             complement(7689..8605)
                     /locus_tag="VLC50_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004941537.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase C-terminal
                     domain-containing protein"
     gene            complement(8739..9248)
                     /locus_tag="VLC50_06885"
     CDS             complement(8739..9248)
                     /locus_tag="VLC50_06885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF27226.1"
                     /translation="MSELLTPGRRRAMLVAIWLLAIAVGASLAWVAVDRAGRAVLDPS
                     TAAGALPVPTASPSAPSSITPLPTATPPVPRRPSVTPDPSTTRASQPGPVARTQLTDG
                     GTVAVTCRGGAAELSYATPRAGWLVELRDRGPREVRVRFERAGSGKHSHIDVRASCSE
                     GSPAFDVED"
     gene            9287..9976
                     /locus_tag="VLC50_06890"
     CDS             9287..9976
                     /locus_tag="VLC50_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HSF27227.1"
                     /translation="MPSILLIEDDPAIRRELVEVLRGRGHAIATAPNGMDGLRLAVDE
                     RPDVVLLDLGLPDLDGGTVLTMLRAVSDVPVVVVTARDGDANLVRALDAGADDYVVKP
                     FTVEHLDARLRAVLRRTNASPDRDEVLRVGGLEIDARARTACLAGTELDLSRKEFDLL
                     HLLARRAGEVVSKRELLAEVWHQAWGGGDRTVEVHLSWLRRKLGENAAHPRYLHSVRG
                     VGIVLKAPPSP"
     gene            9973..>10209
                     /locus_tag="VLC50_06895"
     CDS             9973..>10209
                     /locus_tag="VLC50_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017973264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HSF27228.1"
                     /translation="MRRSLRLLVAATTSAVALSLLIPLGLLVRTLAEDRAVQAAAEEA
                     AALAAVVATLPAGADAERLVELADERSALIVSVRL"
BASE COUNT         1452 a         3692 c         3582 g         1483 t
ORIGIN      
        1 tcgttgaccg cctcttcgag cacgacggcc cggccgaagc gctggtcgat gatccgggcc
       61 ggcaccttgc ccttgcggaa gccggggacg ttgacctgac cggcgatgcg cttgtaggcg
      121 gcgtcgaggc tcggcttgag ctcatcgaag ggcacctcga cggtgagccg cacccgggtc
      181 gggtcgaggg tctcgacatc ggtcttcacg gcggctggct ctcctcgcgg atcgggtgct
      241 cggttgcggc gcacggggtc atgctggccc gccccgggcc gcgaaaccgc gaccagaggc
      301 gtcgtggtcg gggcggggag actcgaactc ccgatctcct gcacccaaag caggcgcgct
      361 agccactacg ctacgccccg cggcgcctgg cagagtctag aggctccccc ggggtgacac
      421 gcacgtccgt cgtacggcgt cactcccgct cggggcgtgt cgctaggctg ccacccgtcc
      481 gcgggtgtag ctcaatggca gagccccagc cttccaagct ggtcatgcgg gttcgattcc
      541 cgtcacccgc tccgcatgga gcccccggtt ttcggggatc cgaccagtgc aggcccggcc
      601 tgctgcaaca gacgaggcgc tcagctcacg tccttggcgg cctcctggac cgccttgtcg
      661 acgtcttccg gtctgacgtc gaggtccttg gtgacgtgct tgtcggcctt gagccctgcc
      721 ttgtcgacgg cctgacgcag cgtgctctcg cccacgatca caagcgcggc ccgcccttcg
      781 tcgatgatct ccccgaactc cttgatgtcg gcccgggaca tgccgcgcca taggtgcccg
      841 cccacaccgc cgaccgccgc cccaaccagc gcggagccga tgatcgaggg cggaaacagg
      901 atcccgacga cggcaccaac ggcagcaccg ccccacgctc cgcggcgggt cgcggtctcg
      961 tccttgttga cgtgcacctt gccctcggcg tccttggtga tcacggcagc gtcgtacgtc
     1021 ccgatggcgc cgacgtcatg caggtccttg acgacggcgt agtcgcctcg cgccgcaccc
     1081 tcgttcgggt aggtgccgat atagatgaat accgcttcgg ccttggccat gtcctccgcc
     1141 ctcctgccgg gatcggacag cggtgcttcg acctcaggcc atctcgggca cgtggaggtg
     1201 tgcgaaggcc acctcgatct cctcgacgtc gtcgaccgtc gcgccgagct cgctggcgac
     1261 ccgctcgcgg atgcccttgg cggcgtcgcg agtggcttcc agggctccac ggctctcgaa
     1321 gacgacggtc ccgaccaccc ggcccgcatc gcggtcgatc atcaggctgg agctgcagaa
     1381 accgtccatc gccgcgaggc gcgggagcac gaccatcttg taggtgtcga tcgcccgatc
     1441 ggccgttgca ggatcgccgc tgagccaggt gaggcgtgcg caggcgcctt ccggtgcggc
     1501 gcggtcgcgg tgcaccaccg ccacctccca ctggtgcacc tcgctgccgc tggcgcccag
     1561 ggtctgttcg gcccggtccc gcagtggcac gacctgctcg gcgctggccc gcatggcgtc
     1621 ctgggagcgc cacgcggtgg tggcgatgca gcggccggag tcacggttga cgaggagcga
     1681 catcccgaca cacccgtcga tctcggacgc tgccggaaag atgtcgtcgc ggacaacggc
     1741 gatgccggcg tcgatcttcg cgggatcggc ctgcacctcg gtcgtacgta caaacacgac
     1801 gtgctcccat ctgctgacgg cagcgtcccg ccggcgctga cctttgcaca cacgctccgt
     1861 ccaccacgcg ggcgagtcaa ggggcactgc tcagtgaagc gtggggcggt agacgagctc
     1921 ttgagtgcga ccgtcgagcg tctggctctc gaccagctcc agatcgaagt cggctgcgcc
     1981 tcggaagatc gggtccagcc cggtctggcc ggtgacgaca gggaagatcg tcacctgaac
     2041 gaagtcgacg agcccggcag ccatcagcgc ccggttcatc gacaggctgc cgtgcgagcg
     2101 caacggcaca tcggactcct ccttgagctc ggcgacgacg tcgagggcgt caccgctcac
     2161 gaggttcgcg ttcggccagt cgagaggacc ctgcagcgtc gtcgacacca cggtcgccgg
     2221 catgttcctc atccgagtga cccatgggtc acgcacgtcg ggatcctcgg acgccagcag
     2281 ctgcacgaac gctcgatagg tgttggcccc gaacaccatt cgctgctccg cgtcgtagag
     2341 ggcaagacgg tggtccagca gctcggggcc ctgcttgccc cagtagccgg tccagttggc
     2401 gctggccccg ccgtagccgt cgaggctgga gaagacgtcg aaggtgtagg tagcgctcat
     2461 gtggctcttc agcggtcagg aggaatccct ccgtcagcgg cctccgcggg agcggcgcgt
     2521 gctcgactcg ttggccgact tgatggcgtc gaccagctcc cccttgctca tcgccgaccg
     2581 tccgcggatc tcgagacgct tggccacctc catgaggtgg ctcttgctgg cgtgggtgtc
     2641 gacccctcca gccgtccgtc cgccacgacg ggcgggcccc ccgcggcgag ccgcctgcga
     2701 gtccgagggt cccttggccc cgccgggctt ctcctcccag tggtccccga ccttttcgaa
     2761 gctgtgcttg agggcggcga aggccgtgcg gtgtgcccgc tccccctcgc cgtactcgtc
     2821 gacggcagcg tcgtgcgtct tggcccaggt gcgctgcgcc ttggcagagg accggcgaag
     2881 cgttgagggc atgtccttgc ttgcgggcat gactcactca cccctcggct ctgtggctca
     2941 ggacgagccg cctggttcat ttctggagag cgggatggag ggctgctggg cctctcctgt
     3001 ctcaggcgtc gtctctcgcg tcgcatccgg tgcgggctcg cggtcgctca ccccttgagg
     3061 ggactgcgac gggacggtcc cctgcgcctc gcgggggacg cggtgctgcg gaatcctcgg
     3121 ccttgcttga cggatcagca cgaggccgat caccgccacg gccattgtca cgagcccggc
     3181 gacgaccaac cacagagccg gcaccgtcca ggactgtccc agcgcactca cgtcgacatc
     3241 ggtcgtgttc tgcacgatga gaatgaccgc tgccgcgacg gaaatgatga tcagcagaaa
     3301 tccgagaatg atcacgacga cctccgctgt cgagtcagac tctaatcctt gaagacatcc
     3361 ttgatctttt caccggcctg cttgacatta gcctttgtct cgtccttctt tccttccttc
     3421 tccatcgact cgttgccggt catctcaccg accttctctt tggccttacc tgccatctcg
     3481 tcggccttgt tctggagctt gtcctctgcg cccatgatca acctccgatg attgcgacga
     3541 cccctcgaac gtacgccggt gtccccagca ccgaaagagc gtccgtgggt caaatcggtc
     3601 tgacgagcga gtcgacctca caatccttaa agatgcaacg gcagggtgga gaggcaccta
     3661 tcctgggcat ccggatcaac gctccaggag gtcctcatga tcattctcgg ggttatcctg
     3721 ctgataatcg gacttgtcgc gggaattggc gtcttgtgga cgattggcat cattcttgtg
     3781 gtcatcggtg cggtgctctg gatcgccgga gccactggcc acgcagtggg tgggcgcagc
     3841 cactactggt gagttcagcc ttctcggaag gcgcttgagt cgctcggcgc gtcgcagacc
     3901 gcctcggtga ggctcgccgc aacctcgcgc agcttgagat tgcgatgctg cgatgccgag
     3961 cgcaggatcg caaaggcggt gtctgccgag catcggttct gcgccatgat gatgcccatc
     4021 gcctgaccga tcgtgccacg cgaggccagt gccgacttga ggtgcgcgtt ggcctgtacc
     4081 tgctcggcac agcgtctgct cagggccagg aggtgcgcta gctcttgagc gcaccgcgcc
     4141 attgctgcca aggacgacgg gtcccatgca tcaccggttt ccgagtaacc caccaggagg
     4201 atgatcgacc cgtcgaccac ttgggcaggc aatgcggcag cagagcgaaa accgtgggag
     4261 cgcgccagat ctgcccacac cggccaacgt tgatcgttct cgaggtcccg caccagcacg
     4321 atgcggtccg tcgcgagcgc attcactccc gggccctgac ctgacgagtg gcccactgac
     4381 tcgcagtcga cgcaacgccg gtcgctgctc gaccgatcag ccacgctgcc ccgctcgatg
     4441 gtggtgaccg caacctgtga catgggcgcg agccggctgg ccgcggtaat cgtcacatcg
     4501 cgcacgaagc gctcggcgca ggccgtctcg tcgagtcgat ggctacccgc ggccgtggca
     4561 tcccacaggc gtggcgcgct cacatgatca cccccggtcg tcgacgcaat cctcggccag
     4621 gatgagccgg ttcgagttca gcgcgcaatg gccgccacgg accaaacact cgccctccgc
     4681 cagcaccagt cacccgtgag tgccatagtg cttggtgttc ggtcgagcat tcccgagact
     4741 cgcgcggatt gtctggcgat tgtctggcac gcaccgtcgg ccggttcaga agcgagttgc
     4801 gccaacccgc ctgaggcaac gctcgcccgc ggcgagcacc tcgtcgacag tgatccgttc
     4861 cagagcgggg tcgatcaccc gacggtgcgg gtcgcccggt gccgctggtg acccgtgcca
     4921 gagcacctgg tgacgctcgg gatcgagccg cggtccccac agccgcggag cggtcggccc
     4981 gaacagcagc accgaaggcc gtgcccaggg aggtggccag gtgggcgatc ccggtgtctc
     5041 cgcagaccaa cagccgtgct ccggccaccg atgcggccag cgccggcagc cagggcccgc
     5101 acgcgtcgat gccggccgcg gtcaccaccg cctgcggggg cccgaccatc tccgacagct
     5161 cacgcaccgc ggcggccgcg gtgtcggcca ggtccacctc gacactgggc actccggccg
     5221 acggcgactt caggtcgagc accgccggac gcccaccggc ctcgccgacg gcctcgacaa
     5281 ccgccgcgcc gagtccgctg gcccctccgg tcacgtagac ggtgccgacg ggccgggcgc
     5341 ggtaccgagt cgctgtcatg acacccccag ccgttggatc aacgaggtcg tggagcgacc
     5401 tccgagatag ggcaggacga cgacacggcc gccgtgccgg cggaccacct cggcctccgg
     5461 caagggtgtg ccgtcgtagt cgccgccctt gacccacacg tcggggcgca gcacctccaa
     5521 caccgcccgc gggtcgtcct cgtcaaagac gacgaccgcg tcgacgcagt cgaaggcgcg
     5581 agcaccctgg cccggtcggc ggccgggacg agcgggcgtc cggtcgcgcc ggcaccaggt
     5641 gcccgaccgg cggcagctgc tcccgcagcg ccagccggcc gtcgtcgccg ggtgccagcg
     5701 cgccgccggc cgcctcggcc agcgccagcg cggccaccac ctcgtgcgcg tcggggtcac
     5761 cgcccacccc accgcggggg tggcgcaggt ccagcagcga gcccgggtag tcatggctgg
     5821 tcgcggcgac gaagccgatg ccggccagca gcgccatcgg cagcggactc tggtgggccg
     5881 aggtgaacac gatggcccgg tcatagcggc gccggcgcaa cgtggccacc agcgcgtgca
     5941 cggccgactc gcggaccggt ggcgcggtga agccggtcca gggggcgtcg tactcgagca
     6001 cgtcatcgac gccgggcagc agctcggccg cggcgcgccc gcgtggggag accagcaggt
     6061 cgagcatggt cgcggtggcg gccagcgccc gtacggcggg gccacagagc agcacgtcac
     6121 cggcactgtc gaggcgtacg gcgagcaccc ggctcatcgc gacaccccca cgaggccggc
     6181 aaccgcgtca gtcagcgtgg cggcgactcc gaccgtggct ttggccgcgc tcacctcgtc
     6241 cggtcgggtg agcggggctg ggacgagcac cgcacgggct ccggccgcga ccccggcccc
     6301 gacgtcggca ccggtgtcgc cgatcaccag gcactcggcg ggcaggatcc cgaggcgcgc
     6361 ggcggcgcgc agcaccagcg ctggaccggg cttgcggcac gggcagcccg cgtccgggcc
     6421 gtgcgggcac tgctcccaca cgtcgaaggg ccccagctcc gccatgatgc gcgcgttgac
     6481 ggcccgcacc tgcgtcgggg tgagccaccc cagtgccagc ccggactggt tggtgaccac
     6541 gcctaccgcc agcccggcgg cccgcagccg gtcgagcacc gcgcgggcgt cggccacgac
     6601 gaccacccgg tccgggtcgc cgttgtaggg cacgtcttgg accagggtgc cgtctcggtc
     6661 caagagcacg gcccgcaccg gcggcggcca gggtggcacc gcgcgggcat gacgcagcgt
     6721 gccgccgacc cggtgggcca gcgcgaccgg cgggatcacc gcactggtgg tcgtcatccg
     6781 ggcccactca ccgacccagc cgggctggcc ggggcgcggc ccgtcgacga gccggcggac
     6841 caggaagtcg gcgtagaggc tcagccacag cagcccgccg gcggtcgcca accggtgacg
     6901 tcgtacggcg cccgcgccgg ctgccagcgc gccggccgcg accgtcgcgg cgtgccagcg
     6961 ggcccggccc accccggtct gcgcccgggt gcgccagcgc gggccgtgca gggtccgcat
     7021 gagcgcgtcg tcggctcccc cgacctgcgt gcgcacgctg acccagaagt cggcggggcg
     7081 caccgggtgg tgggtgatgc ggtgaccacg gcgcagcgtc cagccctgcc cgcgcacccg
     7141 cagcgcgagg tcggcgtcct cgcggtaggg gcgagggaac cgttcgtcga agccaccgac
     7201 ctcggccaga gcccggcgac ggtaggccat gtcggcggtg gcccagcggg cctgctcgag
     7261 tccggcggtg ttgcgctccc agtccgactg gggccggtcc gccgaaaccg gcacgtgcag
     7321 ccgggcctgc accccggcca ccgacgacgg agcccggtcc aggtcggcgg ccagctcgtg
     7381 cacccactgc ggtggcagct ccacgtcgtc gtcgagcagc accacccact cggtcctcac
     7441 gctgcgccac ccggcgttgc gggccgccgc cggcccccgc ccgcccgacc ggatcacgcg
     7501 ggtggggcac ggcagcggtg gcagcgccag cggcggtggc gccgctgcgc gaagcggccg
     7561 gtcgtcgacg acgacggcct cgaccggggc cgggtgctcg tggtcgaacg gctggccggc
     7621 gaggctggtc agcagcctgg tcagcgaggc gcggccgatg gtgggcacga ccaccgcgta
     7681 gtcgaggctc atcgcgcacc cgcctgggtg cactcgcggg tgccggcgta gagcatcgac
     7741 cggcgcacga ggaacgggcc gagcaccagc gcgtcgacca gcgcggagcc gaacagctcg
     7801 agcgcctcgc gcgggtcatc gaccatcggc cggccggcgg tgttcaggct cgtgttgacg
     7861 accaccggca gcccggtgcg ggcctcgaac gcgcgcagca tcgcggccag ccgcggctgc
     7921 gtcgccgggt cgacggtctg aacccgggcg gtgccgtcca cgtgcaccac ggcggggatc
     7981 cgctcacgcc agcgcggcgc gaccatgtgc acgaagagca tgtaggggct cgggatcggg
     8041 ccgtcggcga agatctcgcc ggctgctcgg ccagcaccat cagcgccacc ggccggaacg
     8101 gttcgcggcc cttgacgtcg ttgagccggg ccaggttggc caggtggccc gctcaccgcg
     8161 caccgtcgag cacgagcacg ctggcatcgc cgtacggcgc ggcgagcgcg gccgacgccg
     8221 cgtgggcgac gtggtggggc acgaagcgca cccgggccgg gtccgggccg ggcagcgcgg
     8281 cggcgaggaa accgggggcg tgctcggcgt acgtcgtacg caggtggtcc cacgggtcga
     8341 gcagaccgag ctcgtcggcc ggccgcgaca gcgccgggtc gaacgagtag gcgaccgggt
     8401 cgagctggtc ggggcgcagc cccgccacgg acagacacca ccgcatcgcc agctcgggca
     8461 gctcccaggc cgagaacggc accgggcgct tgccgtgctt gcgccggctg aagcgctcct
     8521 cctcggcggc cgccacgatc gtgccgtcga cgaccagcgc cgcggagggg tcgtggtaga
     8581 tcgcgttgac gccgaggacg cgcatgctcg tccgacctcc gggtcgccga catgagcgcc
     8641 gtcttcactc ggggccactg tctcccggca gaagcgcttc tcgcaggaga accacttcat
     8701 gcgggaacgg agtgacctcg gtccgcaatc aaggggcctc agtcctcgac gtcgaaagcg
     8761 ggcgacccct cagagcaaga cgcccgtacg tcgatgtggg agtgcttgcc ggagccggca
     8821 cgctcgaacc gcaccctgac ctccctgggc cctcggtcgc gcagctccac gagccacccg
     8881 gcccggggcg tcgcatagct gagctcggcg gcgccgccac ggcaggtgac agcgaccgtc
     8941 ccgccgtctg tcagctgggt ccgtgcgacc ggccccggct gggaggcccg cgtggtggac
     9001 gggtcggggg tcaccgacgg ccggcggggc accggcgggg tggccgtcgg gagcggggtg
     9061 atcgaggacg gggcggacgg cgaggcggtc ggcaccggca acgcgccggc cgccgtgctc
     9121 gggtcgagga cggcgcgccc ggcgcggtcg accgcgaccc aggcgagact ggcgccgacg
     9181 gcgatggcca gcagccagat cgccaccagc atggcccggc gccggcccgg ggtgagcagc
     9241 tcgctcatcg gctcctcacc ctcgcgcgcc gctgtaacgt cacctcgtgc ccagcatcct
     9301 cctgattgag gacgatccgg cgatccgccg cgagctggtc gaggtgctgc gcgggcgtgg
     9361 gcacgcgatc gccaccgccc ccaacgggat ggacggcctg cgtctggcgg tggacgagcg
     9421 ccccgacgtc gtcctgctcg atctggggct gcccgacctc gacggcggca ccgtgctgac
     9481 catgctgcgt gccgtcagcg acgtcccggt cgtcgtggtg accgcccgcg acggtgacgc
     9541 caacctggtc cgcgcgctgg acgccggcgc cgacgactac gtcgtcaagc ccttcacggt
     9601 ggagcacctg gacgcccggc tgcgcgccgt cctgcgccgt accaacgcct cgccggaccg
     9661 tgacgaggtg ctgcgggtgg gcgggctcga gatcgatgca cgggcccgga ccgcctgcct
     9721 ggccggcacg gagctcgacc tgagccgcaa ggagttcgac cttctgcacc tgctcgcacg
     9781 ccgggccgga gaggtcgtgt ccaagcgcga gctgctcgcc gaggtgtggc accaggcgtg
     9841 gggaggcggt gaccgcaccg tcgaggtgca cctgtcgtgg ctgcggcgca agctcggcga
     9901 gaacgccgcg cacccgcgct acctgcacag cgtgcgcggg gtgggcatcg tgctcaaggc
     9961 accgccctcg ccatgagacg gtcactgcgc ctgctcgtcg cggccacgac ctcggccgtc
    10021 gcgctgtcgc tgctcatccc gctggggctg ctcgtgcgca cgctggccga ggaccgagcg
    10081 gtgcaggccg ccgccgagga ggcggcggcg ctggccgccg tggtcgccac gctgccggcg
    10141 ggcgccgacg ccgagcggct ggtcgagctg gccgacgaac ggtcggcact catcgtgtcg
    10201 gtgcggttg
//
LOCUS       DATIBB010000175         2453 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_238123, whole genome shotgun sequence.
ACCESSION   DATIBB010000175 DATIBB010000000
VERSION     DATIBB010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2453)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2453)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2453
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_238123"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..656
                     /locus_tag="VLC50_06900"
     CDS             <1..656
                     /locus_tag="VLC50_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016438254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HSF27229.1"
                     /translation="DDCLVLARDLSERYPVALVNSVNPFRIEGQKTAAFEIVDELGDA
                     PDIHCLPVGNAGNITAYWKGYREYADDGPATRWPRMWGFQAAGAAPIVRGEPVTSPQT
                     VATAIRIGNPASWKQALAARDESDGLIDAVSDEAILAAHRLLSAEEGIFVEPASAASV
                     AGLLQCHEQGLLTPGLRVVCTVTGHGLKDPQWAIKAAPGPIGIPVDVERAASELGLA"
     gene            659..1552
                     /gene="thrB"
                     /locus_tag="VLC50_06905"
     CDS             659..1552
                     /gene="thrB"
                     /locus_tag="VLC50_06905"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014176389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HSF27230.1"
                     /translation="MGQTAAVRVRVPASSANLGPGFDTLGLALTLYDEVEVALVPGSG
                     PLDVEVVGECADTVPRNASHLVARAVATACEGQLPAGLRLRCVNRLPHGRGLGSSAAA
                     IIAGIIAGQELVGCGRDNDTALQLAATMEGHPDNVAAALLGGATIAWTDAQAQAHAVR
                     IDVHPEVRAVALVPPVPVATRTARALLPAQVSHGDAAANAARAALLVEALGHRPDLLL
                     EATTDRLHQVYRTPAMPESVALMDSLRGRGVAAVVSGAGPTVLALTDPDGVDAVVAAA
                     PAGWRAFALEVDRVGATVVSG"
     gene            1944..>2453
                     /locus_tag="VLC50_06910"
     CDS             1944..>2453
                     /locus_tag="VLC50_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013475353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rho termination factor N-terminal
                     domain-containing protein"
                     /protein_id="HSF27231.1"
                     /translation="MSESTDFVQQSAPTGRVDAQDGPSTTTTSRPRRRSGGLAALVLP
                     ELQALAADLGITGTTRMRKSQLIEVITARQAGGSAPATSVAGSAGARAASTAHAATAR
                     AASARSASAPATTVVATPASEAAATAPVAAAPATERAPGPDRATGETRSAEPSTETGH
                     EAGHEAGHET"
BASE COUNT          312 a          937 c          908 g          296 t
ORIGIN      
        1 tcgacgactg cctggtgctg gcccgcgacc tgtccgagcg ctacccggtg gcgctggtca
       61 actcggtcaa cccgttccgg atcgaggggc agaagaccgc cgcgttcgag atcgtcgacg
      121 agctcggcga cgcgcccgac atccactgcc tgccggtcgg caatgcgggc aacatcacgg
      181 cctactggaa gggctatcgc gagtacgccg acgacggccc ggccacccgg tggccgcgga
      241 tgtggggctt ccaggccgcc ggggcggcac cgatcgtgcg tggggagccg gtgacctcgc
      301 cgcagacggt ggccaccgcg atccggatcg gcaacccggc ctcctggaag caggcactcg
      361 cggctcgcga cgagtccgac gggctcatcg acgcggtcag cgacgaggcg atcctagccg
      421 cgcaccggct gctcagcgcc gaggagggca tcttcgtcga gccggcctcc gcggccagcg
      481 tcgccggcct gctgcagtgc cacgagcagg ggctgctgac gcccggtctg cgggtggtct
      541 gcacggtcac cggccacggc ctcaaggacc cgcagtgggc gatcaaggcg gcgccggggc
      601 cgatcggcat cccggtcgac gtcgagcgcg ccgcgtccga gctcgggctc gcctgaccgt
      661 gggccagacc gccgcggtgc gggtgcgcgt gcctgcctcc agcgccaacc tcggcccggg
      721 gttcgacacc ctgggtctcg cgctcaccct gtacgacgag gtcgaggtcg ccctggtccc
      781 ggggtcgggc ccgctcgacg tcgaggtcgt cggcgagtgc gccgacaccg tgccgcgcaa
      841 cgcctcccac ctggtcgcgc gggcggtcgc gaccgcgtgc gagggtcagc tgccggccgg
      901 gctgcggctg cgctgcgtca accggctgcc gcacgggcgg gggctgggct cctcggcggc
      961 cgccatcatc gccgggatca tcgccgggca ggagctggtc ggctgcggtc gcgacaacga
     1021 caccgcgctc cagctggccg ccacgatgga aggccacccc gacaacgtgg ccgcggccct
     1081 gctcgggggc gccactatcg cctggaccga cgcgcaggcc caggctcacg ccgtacgcat
     1141 cgatgtgcat cccgaggtgc gggcggtggc gctggtcccg ccggtgccgg tggcgacccg
     1201 cactgcccgg gcgctgctgc ccgcgcaggt cagccacggc gatgccgccg ccaacgccgc
     1261 ccgagcggcg ctgctcgtcg aggccctggg ccaccggccc gacctgctgc tcgaggcgac
     1321 caccgaccgg ctccaccagg tctaccggac ccccgccatg cccgagtcgg tcgcgctcat
     1381 ggactcgctg cgcgggcgag gcgtcgcggc ggtcgtctcc ggcgccggcc ccacggtgct
     1441 ggcgctgacc gacccggacg gggtcgacgc ggtcgtggcc gccgccccgg ccggctggcg
     1501 ggcgttcgcg ctggaggtcg accgggtggg cgccacggtg gtctcgggct gagccgtgct
     1561 cgacgcgcgc gcggggacgt ggatctgtta gtgtgtggaa cgcaccggat gcctcgcttg
     1621 gctcggtgct ctgccacgac tccccgggag ttctttcccg cgtccgacgt gccctccttc
     1681 gtccgacacg tggctcccga tcccgccctg tcgcccttga ggcaacgcgg cggtggagca
     1741 ggggagacgc caatgagccc ggttgatcgc ggcccgcgtg acaggtggcc gctgcgtcca
     1801 ctcgaccggc acgccaccag atccaccacc ccgcttcccg gcggggagcc tgagccgtca
     1861 cccgcgggtg cgggccggct cggctagctc taccggccgc gcgaaagaca acggcggccg
     1921 tgcgatgagg ggaaggaccc ttagtgagcg agagcaccga cttcgtgcag cagtccgcgc
     1981 ccaccgggcg cgtcgacgca caggacggcc ccagcaccac cacgacttca cgacccaggc
     2041 gccgcagcgg cggcctggcc gcgcttgtgc tccccgagct gcaggcgctg gccgccgacc
     2101 tcggcatcac cggcacgacc cggatgcgca agagccagct gatcgaggtc atcacggccc
     2161 ggcaggctgg cggctccgcg ccggccacca gcgtggccgg ctcggcgggc gcgcgcgcgg
     2221 cgagcacggc ccacgcggcg accgcgcggg ccgccagcgc gcgctctgcc agcgcaccgg
     2281 cgacgactgt ggtcgccacg ccggcctcgg aggctgccgc gacggcgccg gtggccgcgg
     2341 cgcccgcgac cgagcgggca cccggcccgg accgggcgac cggcgagacc cgctcagccg
     2401 agccgagcac agaaaccggc catgaggccg gtcacgaggc gggtcacgag acc
//
LOCUS       DATIBB010000176        19973 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_636118, whole genome shotgun sequence.
ACCESSION   DATIBB010000176 DATIBB010000000
VERSION     DATIBB010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 19973)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19973)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19973
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_636118"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..780)
                     /locus_tag="VLC50_06915"
     CDS             complement(<1..780)
                     /locus_tag="VLC50_06915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF27232.1"
                     /translation="MDRQHRARSVRTVRRAVGVLSVCLLLVAGLVWQGAVRQRPAQSG
                     TVGTSQPATVSAIPSASATPTAAPTEGASAPRPSTTANGSAPSRAEERRAALTKLLDA
                     RSAAVAHRDVDAFLATVDPTAADYRTQQTTLFANLRRLDPVVWRYEYGGEHGEVPAGQ
                     SAKSGSVGYLVRVQLHYRLGPVDPVDVVRQQWVVARQHAGRWVLAGPGDDVGEGHESD
                     VDAWDLGPVTVARGDASLVVARSGGGSGGPRVRPASSYVEEV"
     gene            complement(815..1915)
                     /locus_tag="VLC50_06920"
     CDS             complement(815..1915)
                     /locus_tag="VLC50_06920"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HSF27233.1"
                     /translation="MATRRLRCLRHPRHVALSVTAATTSLVVAGALLAGTASADPKPT
                     VAQVEARVAALNVEVEQAAELYNESRDELDTVTKALAKLKERLAATEAKLADAQLSVD
                     AFASATYRSAGVDPTVQLLMSENPDAFLRRASSLNEVSRQQAQALQAMTTVRTRMAQD
                     KLAVAQQLGLQQEAEATLKAAQKRAEAKLAEAERLLRSLKAEDRARIEARRAAERQEA
                     LAQARAAAAAERAAHRTTRSTKRSAPTTTKVAASGRAAAAVKFAYAQLGDRYSWGAAG
                     MSRWDCSGLTMMAYRAAGVSLPHSSRGQAGTVRSIPRSQLKPGDLVFFYSPISHVGIY
                     IGGGKMIDAPHPGASVRITSISSMPFVRAGRI"
     regulatory      complement(1928..2095)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00379"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-AMP (ydaO/yuaA leader) riboswitch senses
                     the signaling molecule cyclic di-AMP; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="cyclic di-AMP"
                     /db_xref="RFAM:RF00379"
     gene            complement(2205..2417)
                     /locus_tag="VLC50_06925"
     CDS             complement(2205..2417)
                     /locus_tag="VLC50_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017933923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF27234.1"
                     /translation="MLIDCDTCAARGPACADCVVTVLLGAPPDGVVFDDDQRAAIDVL
                     ARSGLVPPLRLVVGEAPPEPPAVRAG"
     gene            2582..4405
                     /locus_tag="VLC50_06930"
     CDS             2582..4405
                     /locus_tag="VLC50_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012920418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000027 - ribosomal large subunit assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEDD exonuclease domain-containing protein"
                     /protein_id="HSF27235.1"
                     /translation="MHLQPRWSELTPASTAAGTTSARQATFDELGTPLPEATFVVVDL
                     ETTGGSPAGAEITEIGAVKVRGGQVLAEFQTLVRPSLPIPPFIAVLTGITDAMVAGAP
                     RLETVVPAFLEFLGDAVVVAHNAPFDVSFLQTACTRTGHTWPANPIVDTVRLARQVVT
                     RDEAPNCKLSSLAVLFRAETTPDHRALSDARATVDVLHGLIGRLGNLGVHSVEELTTY
                     SSTVPAATRRKRHLAESLPRTPGVYVFEDADGQALYIGKATNLRARVRSYFTASEPRA
                     RMREMVALATRVRPIPCATPLEAEVRELRLIAAHKPRFNRRSRSPERAIWLKLTAEAF
                     PRLSVVRAVRDDGATYLGPCASTGAAQEAMAALHETFRLRQCTDRLSPGRHSPACALA
                     EMGRCGAPCTGAETVDDYARHVEAARAAMLGDVRPVVETLTRRINLLARAERFEEAAT
                     ARDRLLVFVRAAARTQRLLALTRCPELVAARPHTGGWELVAVRHGRLVGTSRSPRHAA
                     PMPYVDALRASAETVAPGSGPTPAASAEESECVLRWLESPGVRLVHLEGEWTCPAHGA
                     GSVRARLETAAERVPVVPGDETTRHRSKHRRAAPVSRIALA"
     gene            complement(4436..4723)
                     /locus_tag="VLC50_06935"
     CDS             complement(4436..4723)
                     /locus_tag="VLC50_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012949369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HSF27236.1"
                     /translation="MITAIVLVRAEIDRIPEVAEALAALDGVSEVYSVTGDVALIAMV
                     RVREHEQLADVIAGRLAKVPGVTGTTTHIAFRAYSKHDLDAAFAIGIEGAD"
     gene            complement(4720..5793)
                     /gene="trpD"
                     /locus_tag="VLC50_06940"
     CDS             complement(4720..5793)
                     /gene="trpD"
                     /locus_tag="VLC50_06940"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011437499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HSF27237.1"
                     /translation="MGDVDRTWPGLLAALIGGEDLSSDDTAWAMAQILAGEITDVALA
                     GFLVALRAKGETVDEMAGLVAAMLDAAVRIEVPGRVVDVVGTGGDRARTVNISTMASV
                     VVAAAGERVVKHGNRAASSACGSADLLEALGVSLDLDPQAVAAVAGDAGITFCFAQRF
                     HPAMRHASTTRSGLGVATAFNFLGPLTNPAGPRVHAIGVADLRMAPIMAGVLADRGSS
                     ALVFRGEDGIDELTVTAPSTVWVVRAGAVVPDRLDPRDLGIELVDAQALRGADATFNA
                     AVARRIFAGETGPVRDAVLLNAAAALVAAAADGSTVPATPGGVVEEVRAALPRAAEAI
                     DSGAAAATLHRWVEASRRLESTP"
     gene            complement(5799..6239)
                     /locus_tag="VLC50_06945"
     CDS             complement(5799..6239)
                     /locus_tag="VLC50_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019877712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF27238.1"
                     /translation="MSSEPTSDRPQTLTVLVYSHDATTRAKVTMGLGRRPAADLPRIT
                     YIECATEPAVIARLDEGGIDLCVLDGEAAPAGGMGICRQLKDEIFQCPPVLVLTGRVQ
                     DNWLAAWSRADAVVAHPIDPVELAEAAATLLRARVRRLPAHSRP"
     gene            complement(6300..7277)
                     /locus_tag="VLC50_06950"
     CDS             complement(6300..7277)
                     /locus_tag="VLC50_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018785349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HSF27239.1"
                     /translation="MTPVGLGAPVVGAVLAHAGPSNVPPELTLSRLFTAWVFEPIPAI
                     GVALVAGFYLYGVHRLRARGDRWSRWRTFFFVGMGLGIMLVATQSALALYDTTLLSTH
                     MAQHMLLNMIVPIFLALGAPITLALRTLPRRPRSWLLAVIHSRYAKVMTFPVVAGFIF
                     IANPFVLYYTGYYEATLNNDWLHDLNHVHFVLVGCLWFWPLLGLDPMPLRMPYWARMI
                     AVFTTMPFHAWLGIAIMTQTTVIAEGWYSGMVRTWGDSMLGDQATAGGILWTTGEIVS
                     LIVFSVLFVQWVRYSEREARRIDRQLDLAEARAEARAAAEQARAGGTTG"
     gene            7352..7996
                     /locus_tag="VLC50_06955"
     CDS             7352..7996
                     /locus_tag="VLC50_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003955635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-copper oxidase subunit III"
                     /protein_id="HSF27240.1"
                     /translation="MAAVASATAVPSPAHAQVNRPNMVSVGTIIWLSSELMFFAALFA
                     IYFTIRSVSPDLWVTEPALLDVPFSAFNTTVLVLSSVTCQMGVFAAERGQVRRLGRLF
                     EINRWGLREWFVLTFLMGAFFIGGQSYEYSKLITEGLTIPANAYGSVFYLATGFHGLH
                     VTGGLLAFLFVLGRTFVMRRFTHEQATSAVVVSYYWHFVDVVWIGLFATIYLIK"
     gene            8013..8804
                     /locus_tag="VLC50_06960"
     CDS             8013..8804
                     /locus_tag="VLC50_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003969636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HSF27241.1"
                     /translation="MKALSQHRRHPAAIYVLLLVALVATGGLYAVFAPGGQANASASG
                     SANIAVAEGKALFDTNCATCHGFNAQGSSDGPSLIGVGAAAVDFQVGTGRMPATEESA
                     QILSHKVRYTEAEIGQMAAYIASLGPGPAIPSAADLDLSDADMARGGEIFRTNCAMCH
                     NFVGSGGALTEGKFAPAIKDVSAKHIWEAMVTGPQSMPVFGDATMTPDEKQDVIAFVQ
                     GTQAQGNPGGAPLGQIGPVSEGLLGWVVGLGALIGVAVWLGAKAK"
     gene            8801..9853
                     /locus_tag="VLC50_06965"
     CDS             8801..9853
                     /locus_tag="VLC50_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019174964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HSF27242.1"
                     /translation="MSEHTDETAGTPMTSDSNEPGTAPQPEHTDGALQTRFSDPGLPA
                     HRFRLTDTDERAAKRAERQVAGLFGLSVVGTLIFCVGYFSVQLTSTANVGLSTRLLGG
                     GLGLALFSIGLGAIHWAKKLMTDEEIVEERHSTASTDEERAAAAALLAQGNEESGFGR
                     RKLIRNSLVTALGALGLPAIISLRDLGPLPGDRLEHTTWKEGVRIVTDPTGRPVKAAD
                     IPIGGVVHVLPEGIETLEDKAKAAVILIRLEPEEASRHVAKGREDWGYAGIVAYSKIC
                     THVGCPVGLYEQQTHHLLCPCHQSTFDVLNHCEVVFGPAARPLPQLPITVDGEGYLVA
                     QSDFTEPVGPSYWERG"
     gene            9850..11484
                     /locus_tag="VLC50_06970"
     CDS             9850..11484
                     /locus_tag="VLC50_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018557039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase cytochrome b
                     subunit"
                     /protein_id="HSF27243.1"
                     /translation="MSTTQTTGAQPTRGTNKAAAATGRFFDDRFGLAKLGKAGLRKVF
                     PDHWSFMLGEIALYSFIILLLTGVFLTLWFQPSMAEVVYNGSYEPLQGTTMSQAYESA
                     LHISFDVRGGLVIRQIHHWAALLFMAAMIVHMFRVFFTGAFRKPREFNWLIGIGLLTL
                     GIVEGLFGYSLPDDLLSGTGLRILEGVLLSLPLVGTYLTMFLFGGEFPGHDIIPRLFT
                     IHVLLLPAIFLALITAHILFVVYAKHTQYRGPGRQENNVVGYPLLPVYTAKAGGFFFI
                     VFGVTTLLSAFATINPIWLYGAYNPTQISAGSQPDWYMGWLEGLLRAFPAWETTVAGF
                     TIPWNVLVPALLIPGLLFTFWALYPFLEAWVTGDHRHHHLLDRPRNKPVRTAIGAVVV
                     AEFLIMWANGGNDILATTFDMSINSITWATRVLLIVAPPIVFVITKRVCLGLQRRDRE
                     LVLHGRETGRIVRLPHGEFIEIHEPISQVEQYQLTDYESLRPVEAGPAVDDNGVPAPR
                     RRGDRLRARLSRFYFEDRIEAATPAEVHELEAAGHH"
     gene            complement(11576..11986)
                     /locus_tag="VLC50_06975"
     CDS             complement(11576..11986)
                     /locus_tag="VLC50_06975"
                     /inference="COORDINATES: protein motif:HMM:NF025444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4259 domain-containing protein"
                     /protein_id="HSF27244.1"
                     /translation="MAMWGSGPFDCDEALDWFADLEQTPSTELPGALAAALHPGEAET
                     DQRVALAAAVLVAHGCARAVRAGAPEVDEWLGGHRPTVSRQLADEALLVVDAAIDAGV
                     VPGSVPWLSGVRRALEAGSAREQVTPIRPDDSPS"
     gene            complement(12013..13182)
                     /locus_tag="VLC50_06980"
     CDS             complement(12013..13182)
                     /locus_tag="VLC50_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020671830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSF27245.1"
                     /translation="MNKRGVALMSGAHAVDDLYQGAVPALLPFLVAERHYTYAAVAGL
                     TLAATVLSSVAQPLFGWWTDRGARRWMIGVGMSMAALGVALSGLTTSYELTWIVIACS
                     GLGIAAFHPEAARAARQAAGDSNRAMSVFALGGNAGFALGAVITTPLLMAAGVSATVF
                     LALPALVMVGILAARLRVVLDGPAGHPTPDTLPTGEDDWSAFLRLTLVVVARSMLFFG
                     LTSFLALYFIDDLGGSATEGGVALTVFLVSGSVGTLLGGWVADRFGRVLSIRLGLALT
                     APAIAGLVMTTNRPVALVFVVLTGIAVFMPFSVLVMLGQDYLPNRIGTASGVTVGLAV
                     SVGGLATPVLGRLADLTSLRTSLSVLIVLPLVALALSVLIHEPAPRTADSEQLAV"
     gene            complement(13182..13574)
                     /locus_tag="VLC50_06985"
     CDS             complement(13182..13574)
                     /locus_tag="VLC50_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012920403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 4"
                     /protein_id="HSF27246.1"
                     /translation="MKAGGYFFGVLTLFFGTVAVIYWIFSNEHAGTVALALTGGLCLL
                     VAFYLLFTARRLPPLPEDKLEAEVYEGAGEVGFYSPHSWMPLALGAAVAVLFLGLVFG
                     WWLFAIGAVLVALATIGFVFEYYRADAY"
     gene            complement(13571..15265)
                     /gene="ctaD"
                     /locus_tag="VLC50_06990"
     CDS             complement(13571..15265)
                     /gene="ctaD"
                     /locus_tag="VLC50_06990"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009718586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HSF27247.1"
                     /translation="MTAPPVHHKGNAVVRWITSTDHKVIGQLYLATSFGFFLIGGVMA
                     MVMRAELARPGLQFLSNTQYNQMFTMHGTIMLLLFATPLFVGFTNFILPLQIGSPDVA
                     FPRLNMFSYWLFLFGGIITVAGFFTPGGAAAFGWFAYAPLSDSAFSPSIGADMWFLGL
                     AMTGFGTILGAVNFLTTVFTMRAPGMTMFRMPIFTWNVTLTSVIVLMVFPVLAAALLA
                     MEADRKFGAHIFDPDNGGAILWQHLFWFFGHPEVYIIALPFFGIVSEIFPVFSRKPVF
                     GFKGLIFATIAISGLSVAVWAHHMFATGAVLLPFFSFMSFLIAVPTGVKFFNWIGTMW
                     RGSITFETPMLWALGFLVTFLFGGLTGVMLASPPLDFHVTDTYFVVAHFHYVVFGTVA
                     FAMNAGFYFWWPKITGKMLDERIGKVHFWMLFVGFHGTFLIQHWLGVEGMPRRYADYL
                     ASDGFTTLNTLSTLGAFILGASTIPWVYNVWKTAKKAPKVTVDDPWGYGASLEWATSC
                     PPPRHNFATLPRIRSERPAFDLHYPHAAHTDVPVKHRAAATASVGADGSHDGEAEK"
     gene            complement(15301..16044)
                     /gene="coxB"
                     /locus_tag="VLC50_06995"
     CDS             complement(15301..16044)
                     /gene="coxB"
                     /locus_tag="VLC50_06995"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020553005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HSF27248.1"
                     /translation="MASPVRVALVTLVGMLVLAGCSSEELPRLGMPEPVTNQGPRVLL
                     LWQTAWFAALIIGGLVWGMIIWSVIVYRRKHVDDPLPKQTRYNLPIEVFYTVVPLIII
                     AVFFFYTARDETEITHVSANPDVTINVNAFQWSWQFNYVDDDVSVTGSPGLPPTLVLP
                     KGKSVKFELTSEDVIHSFWVPAFLYKLDVVPGHPNEFEIVPEKLGTYVGKCAELCGVD
                     HSRMLFNVDIVEPEQYDQYLASLKAGSAK"
     gene            complement(16199..16969)
                     /gene="aat"
                     /locus_tag="VLC50_07000"
     CDS             complement(16199..16969)
                     /gene="aat"
                     /locus_tag="VLC50_07000"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HSF27249.1"
                     /translation="MPVEPPQSRWAFTLDRAVPGEDLVGAGADLAPGTLLAGYRTGVF
                     PMGLGRGGRGRMGWWSPDPRGVIPLAGLRVSRSTRASVRRFRVTVDADFAAVVAGCAD
                     PRRPGAWITSAVAQAYRQLHELGWAHSVEVWHPDGWLAGGLYGVAIGGLFAGESMFRT
                     PLPQARDASKVALVHLVELLRADEHPDRLLDVQWRTPHLASLGCVEVSRADYLRRLDR
                     ALRLPLPAVLDRPTPYDVPVPPLSPGHRADGVRPHALE"
     gene            17027..18553
                     /locus_tag="VLC50_07005"
     CDS             17027..18553
                     /locus_tag="VLC50_07005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF27250.1"
                     /translation="MTDARDAVDQPGPEPRPRSHRLVPLAAALAVLVVVGLVAVVAAA
                     RPDGPPPLGLTSVAADGAEPGIASYGGSGGYVLEAVLPAGPATAPVHRLPRDADAAAV
                     SRLATALGLPGSPVDTGSRWEISAGARVLRVASTAGAQWTYARDVDRACLPMPGAGPD
                     EPVSCAAVAEPATPPGAPTRPSAAAVSETRAVALAEPVLRAAGLDPAHAVVTGLGPQA
                     LVRVDPMVAGLPTAGWSTAVTVDVDGVVTVSGWLSTTTRTGPDYPVVSARAAFDRLAA
                     SPVPALGMACPEPAVGQSPLCGGPVTVTGATFGLSLEQTTDGPLLVPAWLFEVADSPD
                     PVPVVAVDPAFWGSPADPVPPSGDPDGSGGSGSSGGSVGGGAGVEPVPPSGVEPVPPS
                     GPSGPPISEPGPAVTPPTAAASVASATVGADERTVELAFWGAPDVDGPCGAAYAVSVA
                     QAPDAVVVAVNERPRPISESATCPAIAAERHLTVALDAPLAQRPIIDAATGTPVPLHR
                     "
     gene            complement(18618..18998)
                     /locus_tag="VLC50_07010"
     CDS             complement(18618..18998)
                     /locus_tag="VLC50_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HSF27251.1"
                     /translation="MTEQVETQVAADTAAEAPAHGVILTDVAATKVKSLLEQEGRDDL
                     RLRVGVQPGGCSGLIYQLYFDERTLDGDLVRDFGGVEVVVDRMSSPYLEGATIDFADT
                     IEKQGFTIDNPNAGGSCACGDSFH"
     gene            complement(19093..>19973)
                     /locus_tag="VLC50_07015"
     CDS             complement(19093..>19973)
                     /locus_tag="VLC50_07015"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HSF27252.1"
                     /translation="AGLHLLAADERNPGVTSTYGVGLLLDAALREGAERIVVGLGGSG
                     TNDGGAGMLAALGAGSAHDLARGGLALADLPDAALKGLEDVKDRFAAIELLAATDVDS
                     PLLGLQGASAVFAPQKGASPALAQALEAALGRFTEVVQRTHQSSRDLLTGRPRRLDKE
                     PGAGAAGGLGYGLMLLGGRRVSGVEEVLTAVGFDSVVSAADLVVTGEGRLDWQSLRGK
                     VVAGVARAALEAATPTVVIAGQTMVGRREAMALGLSGTYAVAETTEQVEAAITDPVGT
                     LAARAARVAATWSPGR"
BASE COUNT         2971 a         7160 c         7114 g         2728 t
ORIGIN      
        1 gacctcttcg acgtacgacg aggccgggcg gacccgcggt ccgccggagc cgccgccgga
       61 ccgggccacc acgaggctgg cgtcgccgcg ggccaccgtg accggaccca ggtcccaggc
      121 atcgacgtcg gactcgtggc cctcccccac gtcgtcaccc ggcccggcga gcacccaccg
      181 gccggcgtgc tggcgggcca ccacccactg ctggcgtacg acgtccaccg ggtcgaccgg
      241 gccgagccgg tagtgcagct gcacccggac caggtagccg accgacccgg acttcgccga
      301 ctggccggcg ggcacctcac cgtgctcgcc gccgtactcg tagcgccaga ccaccgggtc
      361 gagccggcgc aggttggcga acagggtcgt ctgctgggtc cggtagtcgg ccgcggtcgg
      421 gtccaccgtg gcaaggaacg catcgacgtc acggtgcgcc accgcggccg agcgtgcgtc
      481 gagcagcttg gtgagcgccg cccgccgctc ctcggcgcgg ctgggcgcgg acccgttggc
      541 cgtggtcgac gggcgtggcg cgctcgcgcc ttcggtgggt gccgcggtcg gtgtggccga
      601 cgccgagggg atggccgaga cggtggccgg ctggctggtg ccgaccgtgc ccgactgggc
      661 gggacgctgc ctgacggctc cctgccagac cagcccggcg acgaggagca ggcagacgct
      721 gaggactcca acggcacggc gcaccgtccg aaccgaccgg gcgcggtgct ggcggtccac
      781 ccccggagtc taggcagccg ggcccagcct gggctcagat ccgcccggcg cggacgaagg
      841 gcatcgagga gatcgacgtg atccgcacgc tggcacccgg gtgcggcgcg tcgatcatct
      901 tgccgccgcc gatgtagatg cccacgtggc tgatcggtga gtagaagaag accaggtcgc
      961 ccggcttcag ctgcgagcgc gggatgctgc gcacggtgcc ggcctgcccg cgggaggagt
     1021 gcggcagcga gacgccggcg gctcgatagg ccatcatcgt cagccccgag cagtcccacc
     1081 ggctcatgcc ggccgcaccc cagctgtagc ggtcaccgag ctgggcatag gcgaacttca
     1141 ccgcggctgc cgcgcggccg gaggccgcca ccttggtggt cgtgggggcc gagcgcttcg
     1201 tgctgcgtgt ggtccggtgc gcggcccgct ccgcggcggc ggcggcgcgc gcctgcgcga
     1261 gtgcctcctg ccgctcggcc gcgcgcctcg cctcgatgcg ggcccggtcc tcggccttga
     1321 gcgagcgcag caggcgctcg gcctcggcca gcttggcctc cgcccgcttc tgcgccgcct
     1381 tgagcgtggc ctcggcctcc tgctgcaggc cgagctgctg ggcgaccgcg agcttgtcct
     1441 gggccatccg ggtgcggacc gtggtcatcg cctgcagcgc ctgggcctgc tggcgggaca
     1501 cctcgttgag cgaggacgcc cgccgcagga acgcgtcggg gttctcggac atcagcagtt
     1561 gcacggtcgg gtcgaccccg gccgaccggt aggtcgccga cgcgaacgcg tcgaccgaca
     1621 gctgcgcgtc ggcgagcttg gcctccgtgg ccgccagccg ctccttgagc ttggccagcg
     1681 ccttggtcac ggtgtcgagc tcgtcgcgcg actcgttgta gagctcggcg gcctgctcga
     1741 cctcgacgtt gagggcggcc acccgggcct cgacctgggc gacggtgggc ttggggtcgg
     1801 ccgacgccgt gccggccagc agcgcaccgg ccaccaccag gctcgtcgtg gcagcggtca
     1861 cgctcagcgc gacatggcga gggtggcgca ggcagcgcag gcggcgggtc gccacgaggg
     1921 gccgtactcc ttcttcctcg cgcgcctacc gggttagctg acgggctcgg gtggggaagc
     1981 tcaccctacg gcgcacgacc ccaacggctc gcgcgccgat tcgccccgga aaaccctggt
     2041 tccccggtcc ggccaacccg gacaaacggg tcgaacggtt cggcgggggc gttcaccgcc
     2101 tcctgggtga aggaccggtc gcgaacgccc agggcgcacc atagtgacca ttccgtaacc
     2161 aaatcaaact ccgtgcgcaa tgtgcccgcg tgtcgcgcgg cggatcagcc ggcccggacg
     2221 gccggcggct cgggcggggc ctcgcccacg accaggcgca gcggcgggac cagacccgac
     2281 cgagccagta cgtcgatcgc cgcccgctgg tcgtcgtcga agaccacgcc gtcgggcgga
     2341 gccccgagca gcacggtgac cacgcagtcg gcgcacgccg gaccacgcgc cgcgcaggtg
     2401 tcgcagtcga tgagcacgac ggcctcccgt cactgggtga gccaccgcct cctggtggct
     2461 ccatgtcggc gcgacgctag aggcggccac cgacagaccc ggcccagcca cgacaaggcc
     2521 ggccgaacca cccccggcgt gccgccacgg cagtgtcgga cccgttacct accgtcaagg
     2581 catgcacctt cagccgcggt ggtccgagct cacccccgcg tccaccgcgg ccggcacgac
     2641 cagcgcgaga caggccacgt tcgacgagct cggcaccccg ctgcccgagg ccacgttcgt
     2701 ggtggtcgac ctcgagacca ccggaggctc cccggctggc gccgagatca ccgagatcgg
     2761 cgccgtcaag gtgcgcgggg ggcaggtgct ggccgagttc cagaccctgg tccggccaag
     2821 cctgccgatc ccgccgttca tcgccgtgct caccggcatc accgacgcga tggtggccgg
     2881 cgctccccgg ctcgagacgg tcgtcccggc gttcctcgag ttcctcggcg acgcggtcgt
     2941 cgtggcccac aacgcgccgt tcgacgtgtc gttcctgcag acggcctgca cccgcaccgg
     3001 ccacacctgg cccgccaacc cgatcgtcga cacggtgcgg ctggcccggc aggtggtgac
     3061 ccgcgacgag gcacccaact gcaagctgtc gtcgttggcg gtgctcttcc gcgccgagac
     3121 gacccccgac caccgagcgc tctccgacgc ccgcgcgacc gtcgacgtgc tgcacgggct
     3181 gatcgggcgg ctcggcaacc tgggggtgca ctcggtcgag gagctcacca cctactcctc
     3241 gacggtgccg gccgccaccc ggcgcaagcg tcatctcgcc gagtcgctgc cgcgcacgcc
     3301 gggggtctac gtcttcgagg acgccgacgg gcaggcgctc tacatcggca aggcgaccaa
     3361 tctgcgggcc cgggtgcgct cctacttcac cgcgtccgag ccccgcgccc ggatgcgcga
     3421 gatggtggcg ctggccaccc gggtgaggcc gatcccgtgc gccacgccgc tcgaggccga
     3481 ggtgcgcgag ctgcggctga tcgccgcgca caagccgcgc ttcaaccgcc gctcccgctc
     3541 gcccgagcgg gcgatctggc tcaagctcac cgccgaggcg ttcccccggc tgtccgtggt
     3601 ccgggcggtc cgcgacgacg gagccaccta cctcgggccc tgcgcctcca caggggccgc
     3661 ccaggaggcc atggccgcgt tgcacgagac gttccggctg cggcagtgca ccgaccggct
     3721 gtcgccaggt cggcactcgc cggcctgcgc gctggccgag atgggccgct gcggcgcccc
     3781 gtgcaccggg gccgagaccg tcgacgacta cgcccgccac gtcgaggccg cgcgcgccgc
     3841 gatgctcggc gacgtccggc cggtggtgga gacactcacc cggcggatca acctgctggc
     3901 tcgcgccgaa cgcttcgagg aggcggccac cgcccgcgac cggctgctgg tcttcgtgcg
     3961 ggcggcggcc cgcacccagc gcctgctcgc cctcacccgc tgccccgagc tggtggcggc
     4021 ccgcccgcac acgggcggct gggagctcgt cgcggtccgc catgggcggc tggtgggcac
     4081 gtcgcgttcg ccccgccatg ccgcgccgat gccctatgta gacgcgctgc gggccagcgc
     4141 cgagacggtc gcgcccggct ctggcccaac accggcggcc agcgccgagg agtccgagtg
     4201 cgtgctgcgc tggctcgagt caccaggggt gcggctcgtc cacctcgagg gcgagtggac
     4261 ctgcccggcc cacggcgccg gctcggtccg ggcccggctc gagaccgcgg ccgagcgggt
     4321 cccggtcgtg cccggtgacg agacgacgcg ccaccgcagc aagcatcggc gcgccgcccc
     4381 ggtctcccgg atcgcgctgg cctagcggag gcgagccccc gcgctcagct cgggctcagt
     4441 cggcgccttc gatgccgatc gcgaacgcgg catcgagatc gtgcttggag taggcgcgaa
     4501 acgcgatgtg ggtcgtcgta ccggtcacgc cggggacctt ggccagccgc ccggcgatca
     4561 cgtcggccag ctgctcgtgc tcacgcacgc ggaccatagc gatgagggcg acgtcaccgg
     4621 tgacggagta gacctcgctg accccgtcga gcgcggccag cgcctcggcg acctcgggga
     4681 tgcggtcgat ctcggcccgc accaaaacga tggcggtgat cacggcgtcg actctagtcg
     4741 gcggctcgcc tcgacccagc ggtgcagggt cgccgccgcc gccccggagt cgatcgcctc
     4801 ggccgcccgc ggcagcgccg cgcgcacctc ctcgaccacc ccgcccggcg tcgcgggcac
     4861 cgtggaaccg tcggcggccg cggccaccag cgcggcggcg gcattgagca gtacggcgtc
     4921 gcggaccggc ccggtctcgc cggcgaagat ccgccgggcc accgcggcgt tgaacgtcgc
     4981 gtcggctccc cgcagcgcct gcgcgtcgac cagctcaatg cccaggtcac gcgggtcgag
     5041 ccggtcgggc accaccgccc cggcacgcac cacccagacc gtggagggcg cggtgacggt
     5101 cagctcgtcg atgccgtcct cgccgcggaa gaccagggcg gagctgcccc ggtcggccaa
     5161 caccccggcc atgatcggcg ccatgcgcag gtcggcgacc ccgatcgcgt gtacccgagg
     5221 cccggcgggg ttggtcagcg ggcccaggaa gttgaacgcc gtggccaccc cgagcccgct
     5281 tcgggtggtc gacgcgtgac gcatcgccgg gtggaaccgc tgtgcgaagc agaacgtgat
     5341 gccggcgtcg cccgccacgg cggcgaccgc ctgggggtcc aggtcgagcg agacgccgag
     5401 cgcctcgagc aggtcggccg agccgcaggc cgaggaggcg gcccggttgc cgtgcttgac
     5461 cacccgctcc cccgcggccg ccacgaccac cgaggccatc gtcgagatgt tgacggtgcg
     5521 ggcccggtcg ccgccggtgc cgaccacgtc gacgactcgg ccgggcacct cgatgcgcac
     5581 ggccgcgtcg agcatcgccg cgacgagccc ggccatctcg tcgacggtct cgcccttggc
     5641 ccgcagcgcg acaaggaacc cggccagcgc cacgtcggtg atctcgccgg ccaggatctg
     5701 cgccatggcc caggcggtgt cgtcgctgct gaggtcctcg ccgccgatca gggcggccag
     5761 caggcccggc caggtccggt cgacgtcgcc catggcgttc acgggcgact gtgcgccggc
     5821 agccgcctga cccgtgcccg cagcagcgtg gcggccgcct cagccagctc gaccgggtcg
     5881 atcggatgcg cgacgaccgc gtcggcccgc gaccacgcgg cgagccagtt gtcctgcacc
     5941 cggccggtga gcaccagcac cggagggcac tggaagatct cgtccttgag ctggcggcag
     6001 atgcccatgc cgccggcggg ggcggcctcg ccgtcgagga cgcacaggtc gatgccgccc
     6061 tcgtcgagcc gggcgatcac ggcgggctcg gtggcgcact cgatgtaggt gatccgcggc
     6121 aggtcggcgg cgggccgacg gcccagcccc atggtgacct tggcgcgggt ggtggcgtcg
     6181 tggctgtaga cgagcacggt gagcgtctga ggccgatcgc tggtgggctc gctggacatc
     6241 aacttctcgc ttcacgggac gccggcttgt gtcgcggcga tggtatcggg cggcggtggt
     6301 cagcccgtgg tgccaccggc ccgagcctgc tcggcggcgg ctcgcgcctc ggcgcgtgct
     6361 tcggccaggt cgagctggcg gtcgatccgg cgggcctccc gctcggagta gcgcacccac
     6421 tggacgaaca gcacggagaa cacgatcagc gagacgatct cgccggtggt ccacaggatg
     6481 ccgccggcgg tcgcctggtc accgagcatc gagtcgcccc aggtgcgcac catgccgctg
     6541 taccagccct cggcgatcac cgtggtctgc gtcatgatcg cgataccgag ccaggcgtgg
     6601 aacggcatcg tggtgaagac cgcgatcatc cgggcccagt agggcatccg cagcggcatc
     6661 gggtcgaggc cgagcagcgg ccagaaccac aggcagccga ccagcacgaa gtgcacgtgg
     6721 ttgaggtcgt gcagccagtc gttgttgagc gtggcctcgt agtaaccggt gtagtagagg
     6781 acgaacgggt tggcgatgaa gatgaacccg gcgaccaccg ggaaggtcat caccttggcg
     6841 tagcggctgt ggatcaccgc gagcagccag ctgcggggcc ggcgcgggag ggtgcgcagc
     6901 gccaacgtga tcggcgcacc cagtgccagg aagatcggca cgatcatgtt gagcagcatg
     6961 tgctgggcca tgtgcgtcga cagcagcgtc gtgtcataga gcgccagcgc ggactgggtg
     7021 gccacgagca tgatgcccag gcccatcccg acgaagaaga acgtgcgcca ccgcgaccac
     7081 ctgtcaccgc gggcgcgcag ccggtgcacg ccgtacagat agaacccggc gaccagcgcc
     7141 accccgatcg ccgggatcgg ctcgaagacc caggcggtga acagccgcga cagggtcagc
     7201 tcgggcggca cgttcgatgg gccggcgtgg gccaggacgg ccccgaccac cggtgcaccg
     7261 aggcccacag gagtcacgcg tgcaggctac gtcgagtcag gttcgcacca tcggggggtc
     7321 gggggtgtcc ggctgggacg gcaaggccat aatggcggcc gtggcgagcg caaccgcggt
     7381 gccgagccct gcccatgcgc aggtcaaccg gcccaacatg gtgtcagtcg ggacgatcat
     7441 ctggttgtcc tccgagttga tgttcttcgc ggcgttgttc gcgatctact tcacgatccg
     7501 ctcggtgagc ccggatctgt gggtcaccga gcccgcgctg ctcgacgtgc cgttctcggc
     7561 gttcaacacc acggtgctgg tgctctcgtc ggtcacctgc cagatgggcg tctttgccgc
     7621 tgagcgcggg caggtacgac gccttggccg gctcttcgag atcaaccgct ggggcctgcg
     7681 cgagtggttc gtgctgacct tcctgatggg cgcgttcttc atcggcggcc agagctacga
     7741 gtactccaag ctcatcactg agggcctgac gatcccggcc aacgcctacg gctcggtctt
     7801 ctacctcgcc accgggttcc acgggctgca cgtcacgggc ggcctgctgg ccttcttgtt
     7861 cgtgctcggc cgcacgttcg tcatgcgccg cttcacccac gagcaggcca ccagcgcggt
     7921 cgtcgtgtcc tactactggc acttcgtcga cgtggtgtgg atcggcctgt tcgccacgat
     7981 ctacctcatc aagtgactgg agcagcgcac tcgtgaaggc cctgtctcag catcgccggc
     8041 atccggcggc gatctacgtc ctgctcctcg tcgcgctggt ggcgaccggt gggctctacg
     8101 ccgtgttcgc gcccggcggc caggccaacg cctcggccag cgggtcagcc aacatcgccg
     8161 tcgccgaggg caaggcgctc ttcgacacca actgcgccac ctgccacggc ttcaacgccc
     8221 agggcagcag cgacgggccg tcgctgatcg gtgtcggcgc cgccgcggtc gacttccagg
     8281 tgggcaccgg tcggatgccg gccaccgagg agtccgcgca gatcctgtcc cacaaggtgc
     8341 gctacacgga ggccgagatc ggccagatgg ccgcctacat cgcctcgctc ggccccggcc
     8401 cggccatccc cagcgccgcc gacctcgacc tcagcgacgc cgacatggcc cgcggtggcg
     8461 agatcttccg caccaactgc gcgatgtgcc acaacttcgt gggctccggg ggcgccctga
     8521 ccgagggcaa gttcgcaccg gcgatcaagg acgtctcggc caagcacatc tgggaggcca
     8581 tggtcaccgg tccccagtcg atgccggtct tcggtgacgc gacgatgacg cccgacgaga
     8641 agcaggacgt catcgcgttc gtccagggca cccaggccca gggcaacccc ggtggcgcac
     8701 cgctcggtca gatcgggccg gtctccgagg gtctgctcgg ctgggtcgta ggcctcggcg
     8761 ctctcatcgg tgtcgcggtg tggctggggg cgaaggccaa gtgagcgagc acaccgacga
     8821 gacggcgggc accccgatga ccagcgacag taacgagccg ggcaccgcac cgcagccgga
     8881 gcacaccgac ggcgccctgc agactcggtt ctccgacccg ggcctgccgg cccaccgctt
     8941 ccgcctgacc gacaccgacg agcgcgccgc caagcgggcc gagcgccagg tcgccgggct
     9001 gttcgggctc tcggtcgtcg gcaccctgat cttctgcgtc ggctacttct cggtgcagct
     9061 gaccagcacc gccaacgtcg gcctgtccac ccgcctgctc ggcggcggcc tgggcctcgc
     9121 gctgttctcg atcggtctcg gtgcgatcca ctgggccaag aagctcatga ccgacgagga
     9181 gatcgtcgaa gagcgccact ccaccgccag caccgacgaa gagcgggccg ctgcggccgc
     9241 cctgctggcc cagggcaacg aggagagcgg cttcggccgg cgcaagctca tccgcaacag
     9301 cctggtcacc gcgctgggcg cgttgggcct gccggcgatc atcagcctgc gtgacctcgg
     9361 cccgctgccc ggtgaccgcc tcgagcacac cacgtggaaa gagggcgtgc ggatcgtcac
     9421 cgaccccacc ggtcgtccgg tgaaggcggc cgacatcccg atcggcgggg tcgtccacgt
     9481 gctgcccgag ggcatcgaga ccctcgagga caaggccaag gccgcggtca tcctgatccg
     9541 gctcgagccc gaagaggcca gccgccacgt cgccaagggc cgcgaggact ggggctacgc
     9601 cggcatcgtg gcctactcca agatctgcac ccacgtcggc tgcccggtgg gcctctacga
     9661 gcagcagacc caccacctgc tctgcccctg ccaccagtcc accttcgacg tgctgaacca
     9721 ctgcgaagtc gtcttcgggc cggcggcgcg gccgctgccg cagctgccga tcaccgtcga
     9781 cggcgagggc tacctcgtcg cgcagagcga cttcaccgag cccgtcggac cgagctattg
     9841 ggagcgtgga tgagcacgac tcagaccacc ggggcgcagc ccacgcgcgg gaccaacaag
     9901 gccgccgcgg ccaccggacg cttcttcgac gaccggttcg gcttggccaa gctgggcaag
     9961 gccgggctgc gcaaggtgtt ccccgaccac tggtcgttca tgctgggcga gatcgccctc
    10021 tacagcttca tcatcttgct gctgaccggg gtgttcctca ccctgtggtt ccagccctcg
    10081 atggccgagg tcgtctacaa cggcagctac gagccgttgc agggcacgac catgtcgcag
    10141 gcctatgagt cggcgctgca catcagcttc gacgtacgcg gtggcctggt catccgccag
    10201 atccaccact gggcggccct gctgttcatg gccgcgatga tcgtgcacat gttccgggtg
    10261 ttcttcaccg gggccttccg caagccgcgc gagttcaact ggctcatcgg catcgggctg
    10321 ctcaccctgg gcatcgtcga gggcctgttc ggctactcgc tgcctgacga cctgctctcg
    10381 ggcaccggcc tgcgcatcct cgagggcgtg ctgctctcgc tcccgctggt cggcacctac
    10441 ctgacgatgt tcctcttcgg cggcgagttc cccggccacg acatcattcc gcggctgttc
    10501 accatccacg tgctgctgct acctgccatc ttcttggcgc tgatcacggc gcacatcctg
    10561 ttcgtggtct acgccaagca cacccagtac cggggccccg gccgccagga gaacaacgtc
    10621 gtcggctacc cgttgctccc ggtctacacc gccaaggcgg gcgggttctt cttcatcgtc
    10681 ttcggcgtga cgacgctcct gtcggcgttc gcgacgatca acccgatctg gctctacggc
    10741 gcctacaacc cgacccagat cagcgcgggc tcgcagcccg actggtacat gggctggctc
    10801 gagggcctgc tacgtgcctt ccccgcatgg gagaccaccg tggccggctt cacgatcccc
    10861 tggaacgtcc tcgtgcctgc cctgctgatc ccgggtctgc tgttcacgtt ctgggcgctc
    10921 tacccgttcc tcgaagcctg ggtcaccggc gaccaccgtc atcaccacct gctcgaccgg
    10981 ccgcgcaaca agccggtgcg tacggcgatc ggcgcggtcg tcgtggccga gttcctcatc
    11041 atgtgggcca acggtggcaa cgacatcctc gccaccacgt tcgacatgtc gatcaactcg
    11101 atcacctggg ccacccgcgt cttgttgatc gtggcgccgc cgatcgtgtt cgtgatcacc
    11161 aagcgggtct gcctcgggct gcagcggcgc gaccgcgagc tggtgctgca cgggcgggag
    11221 accggccgca tcgtccggct gccgcacggt gagttcatcg agatccacga gccgatctcg
    11281 caggtcgagc agtaccagct gaccgactac gagtcgcttc ggccggtcga ggccggcccg
    11341 gcggtcgacg acaacggggt gcccgccccg cgccggcgcg gtgaccggct gcgtgcccgg
    11401 ctgtctcgtt tctacttcga ggaccgcatc gaggcggcga caccggccga ggtgcacgag
    11461 ctcgaggcgg ccggccacca ctgaccgacc cgccccctct ccgcgtgatc atgcacctgt
    11521 gccccgcaca ggtgcatgat cactttcggg ggtacgcggt tgagggcccg acgggtcagc
    11581 tcggcgagtc gtccggccgg atcggggtga cctgctcccg agccgatccc gcctccaggg
    11641 cgcgccgtac gccggacaac cacggcaccg agccgggcac caccccggca tcaatggcgg
    11701 cgtcgacgac cagcagggcc tcatcggcga gctggcgcga gaccgtgggg cgatgcccgc
    11761 cgagccattc gtcgacctct ggcgctcccg cgcgcaccgc gcgggcgcag ccatgggcaa
    11821 cgagcaccgc cgcggcgagg gcgacccgct gatcggtctc cgcctcgccc gggtgcaatg
    11881 cggccgccag cgcgcccggc agctcggtcg acggagtctg ctcgaggtcg gcgaaccagt
    11941 cgagtgcctc gtcacagtcg aacggtccac tcccccacat cgccatgacg ggctccttcc
    12001 tccgcggccg ggtcagaccg cgagctgctc cgagtcagcg gtgcgggggg ctggctcgtg
    12061 gatgagcacc gacaacgcca gtgcgacaag aggcaggacg atgagcacgc tcaggctggt
    12121 ccgcaggctc gtgaggtcgg ccagccggcc cagcaccggg gtggccagcc caccgacgct
    12181 caccgccagc ccgacggtga cgccgctggc ggtgccgatc cggttgggca ggtagtcctg
    12241 gcccagcatg acgagcacgc tgaacggcat gaagaccgcg atgccggtca ggacgacgaa
    12301 gacgagggcg accgggcggt tggtggtcat gacgagcccg gcgatcgccg gcgcggtgag
    12361 ggcgaggccg agccggatgc tgagcacccg tccgaagcgg tcggccaccc aaccgccgag
    12421 cagcgtgccg accgaccccg acaccaggaa cacggtcagc gccaccccgc cctcggtggc
    12481 cgagccgccc aggtcgtcga tgaagtagag agccaggaac gacgtgaggc cgaagaacag
    12541 catcgagcgg gccacgacca cgagcgtgag ccgcagaaag gccgaccagt cgtcctcgcc
    12601 cgtgggcagc gtgtcgggag tggggtgtcc tgccggcccg tcgagcacca cgcgaagccg
    12661 ggcggccagg atcccgacca tcaccagcgc cggcagggcg aggaacacgg tggcgctcac
    12721 gccggccgcc atcaggagcg gcgtcgtgat gacggcaccc aatgcgaagc cggcattgcc
    12781 gccgagcgca aagacgctca tcgctcggtt gctgtccccc gcggcctggc gggcggcccg
    12841 cgccgcctcg gggtgaaagg ccgcgatacc cagcccggag caggcgatga cgatccaggt
    12901 cagctcgtag ctcgtggtca gcccggacag cgccaccccg agcgcggcca tcgacatgcc
    12961 gacgccgatc atccaccggc gggcgccgcg gtcggtccac cagccgaaca acggctgcgc
    13021 caccgaggag agcacggtgg cggccagggt cagcccggcg accgcggcgt aggtgtagtg
    13081 gcgctcggcg accaggaacg ggagcagcgc cgggacggca ccctgataga ggtcgtcaac
    13141 ggcgtgggcg ccggacatca gcgccacacc gcgtttgttc atcagtaggc gtcggcgcgg
    13201 tagtactcga agacgaagcc gatggtcgcc agcgccacca ggaccgcacc gatcgcgaac
    13261 agccaccagc cgaagaccag gccgaggaag agcaccgcaa cggcggcgcc cagggccagc
    13321 ggcatccagc tgtgcgggct gtagaagccg acctcgcccg caccttcgta aacctcggcc
    13381 tcgagcttgt cctcaggcag cggcggcagc cggcgcgcgg tgaacagcag gtagaacgcg
    13441 accagcaggc acaggccacc ggtcagcgcc agggcgacgg tgccggcgtg ctcgttggag
    13501 aagatccagt agatgaccgc gacggtgccg aagaagagcg tcagcacccc gaagaagtag
    13561 cctccggcct tcacttctca gcctctccgt cgtgcgagcc gtcggccccg accgaagccg
    13621 tcgcggcggc ccggtgcttg accggcacgt cggtgtgggc cgcgtgcggg tagtgcaggt
    13681 cgaaggccgg gcgctcggac ctgatgcgag gcagcgtggc gaagttgtgc cgaggcggcg
    13741 ggcaggacgt cgcccactcg agcgaggcgc cgtagcccca cgggtcgtcg acggtgacct
    13801 tcggtgcctt cttggcggtc ttccagacgt tgtagaccca cgggatggtc gaggcgccca
    13861 ggatgaacgc gcccagcgtg gacagcgtgt tgagcgtggt gaacccgtcg gaggccaggt
    13921 agtcggcgta gcgccgcggc attccctcga caccgagcca gtgctggatg aggaacgttc
    13981 cgtggaaccc gacgaagagc atccagaagt gaaccttgcc gatgcgctcg tcgagcatct
    14041 tcccggtgat cttgggccac cagaagtaga agccggcgtt catcgcgaag gccaccgtgc
    14101 cgaacacgac gtagtggaag tgggccacca cgaagtaggt gtcggtgaca tggaagtcga
    14161 gcggcggcga ggccagcatg acgccggtga gcccgccgaa gaggaacgtc accaggaagc
    14221 cgagagccca gagcatcggg gtctcgaacg tgatcgagcc tcgccacatc gtgcctatcc
    14281 agttgaagaa cttcacaccg gtgggcaccg cgatgaggaa ggacatgaac gagaagaacg
    14341 gcaggagcac cgcgccggtg gcgaacatgt ggtgcgccca cacggccacc gacaggccgg
    14401 agatcgcgat cgtggcgaag atcaggccct tgaagccgaa gacaggcttg cggctgaaca
    14461 ccgggaagat ctcggagacg atgccgaaga acggcagcgc gatgatgtag acctcggggt
    14521 ggccgaagaa ccagaacagg tgctgccaca ggatggcgcc accgttgtcg ggatcgaaga
    14581 tgtgggcccc gaacttgcgg tcggcctcca tcgccagcag cgcagccgcc aggaccggga
    14641 agaccatgag cacgatgacg ctggtcagcg tgacgttcca ggtgaagatc ggcatccgga
    14701 acatcgtcat gccgggggcg cgcatcgtga acacggtcgt gaggaagttg accgcgccca
    14761 agatcgtgcc gaacccggtc atcgccaggc cgaggaacca catgtcggcg ccgatgctcg
    14821 gcgagaacgc cgagtcggag agcggggcgt aggcgaacca gccgaacgcg gccgcgccgc
    14881 cgggggtgaa gaagccggcc accgtgatga tgccgccgaa caggaacagc cagtagctga
    14941 acatgttgag ccgcgggaag gcgacgtcgg gcgagccgat ctgcagcggc aggatgaagt
    15001 tggtgaagcc cacgaacaac ggtgtcgcga acagcagcag catgatcgtg ccgtgcatcg
    15061 tgaacatctg gttgtactgg gtgttgctca ggaactgcag gccggggcgg gccagctcgg
    15121 cgcgcatgac catggccatg accccgccga tcaggaagaa cccgaacgag gtcgccagat
    15181 agagctgacc gatcaccttg tggtcggtgc tggtgatcca ccggaccaca gcgttgccct
    15241 tgtgatggac cgggggggcc gtcacggtgg tgtcgagccg cggtggctca cgaagcgttg
    15301 tcacttggca ctccccgcct tgagggacgc gaggtattgg tcgtactgct cgggctcgac
    15361 gatgtcgacg ttgaagagca tccgggagtg gtcgacccca cagagctcgg cgcacttgcc
    15421 gacgtaggtg ccgagctttt ccggcacgat ctcgaactcg ttggggtgcc cgggcaccac
    15481 gtcgagcttg tagaggaacg ccggcaccca gaacgagtgg atgacgtcct cgctggtgag
    15541 ctcgaacttc accgacttgc ccttgggcag caccagcgtg ggcggcagcc cgggtgagcc
    15601 ggtgaccgag acgtcgtcat cgacgtagtt gaactgccac gaccactgga acgcgttgac
    15661 gttgatcgtc acgtcggggt tggcggagac gtgggtgatc tcggtctcgt cgcgcgccgt
    15721 gtagaagaag aacaccgcga tgatgatcag aggtacgacg gtgtagaaga cctcgatcgg
    15781 caggttgtag cgcgtctgct tgggcagcgg gtcatcgacg tgcttgcgcc ggtagacgat
    15841 caccgaccag atgatcatgc cccagacgag gccgccgatg atcagcgccg cgaaccacgc
    15901 ggtctgccag agcagcagga ctcggggacc ttggttggtg acgggctcgg gcatgcccag
    15961 acgcgggagc tcctcggacg aacagcccgc gagcaccagc atccccacga gcgtgacgag
    16021 cgcgacccgc acgggggacg ccacgcggcg tcgggcccga acggtggacg cgcggacaga
    16081 gccgaacgga cgcaaggctg ccttcccacc ctttccgcaa cgctgtgccg gcggggcaga
    16141 ggcacgcaaa accgtgctgt tgcctaccgc gagcagcggt cgtcagtgcg gccacaccct
    16201 actccagcgc atgtgggcgc accccgtcgg cccggtgtcc cggtgagagg ggtggcaccg
    16261 gcacgtcgta cggcgtgggc cggtcgagga ccgccggcag cgggaggcgc agcgcccggt
    16321 ccaggcgacg caggtagtcg gcgcgtgaca cctcgacgca gcccaggctg gccagatgag
    16381 gggtgcgcca ctgcacgtcg agcaaccggt cggggtgctc gtcggcgcgc agcagctcga
    16441 ccaggtgcac gagggccacc ttggaggcgt cgcgggcctg cggcaggggt gtgcggaaca
    16501 tcgactcccc ggcgaacagg ccgccgatcg cgacgccgta gagccctccg gccaaccatc
    16561 cgtcggggtg ccagacctcc acgctgtgcg cccagccgag ctcgtgcagc tggcggtagg
    16621 cctgggccac cgcggaggtg atccaggcgc cgggacgccg ggggtcggcg cagccggcca
    16681 ccaccgcggc gaagtccgca tcaaccgtga cgcggaaccg gcggaccgag gcccgcgtcg
    16741 agcggctgac ccgcagcccg gccaagggga tcaccccgcg cgggtcgggc gaccaccacc
    16801 ccatccggcc gcgcccgccc cggccgagac ccatcgggaa cacgccggtg cggtagccgg
    16861 ccagcagcgt gcccggcgcc aggtcggcgc cggcgccgac gaggtcttcg cccgggaccg
    16921 cacgatcgag cgtgaacgcc caccgtgact gtggcggctc gaccggcacg gtggtcaacc
    16981 tagcgcggcg gcggaaccgg cgagggcccg acggcgtccc agcggcatga ccgatgcacg
    17041 cgatgccgtg gaccagcccg gcccggagcc gcgcccccgg tcccaccgac tggtcccctt
    17101 ggcggccgcg ctggcggtgc tcgtggtggt cgggttggtc gcggtggttg ccgcggctcg
    17161 gccggacggt ccaccgccgc tggggctgac ctcggtggcc gccgacggcg ccgagccagg
    17221 cattgcgtcg tacggcggta gcgggggcta cgtgctcgag gcggtgctgc ccgccggtcc
    17281 ggccaccgcg cccgtgcacc ggctcccgcg cgacgccgac gcagccgccg tctcccggct
    17341 ggccaccgcg ctgggactgc ccgggtcgcc ggtcgacacg ggcagccggt gggagatctc
    17401 cgccggtgcc cgcgtgctgc gggtggcgtc cacggctgga gcccagtgga cctacgcccg
    17461 cgacgtggac cgggcctgcc ttccgatgcc cggcgccgga ccggacgagc ccgtcagttg
    17521 cgcggcggtg gccgagccgg cgaccccgcc cggagcgccg acgcgcccat cggcggcggc
    17581 cgtgtccgag acgagggcgg tggcgctcgc cgagccggtg ctgcgggccg ccgggctcga
    17641 cccggcgcat gcggtcgtga ccggtctcgg gccgcaggcg ctggtgcgcg tcgaccccat
    17701 ggtggccggg ttacccaccg caggctggtc gaccgcggtc accgtggacg tcgacggagt
    17761 ggtgacggtg agcgggtggc tgtcaacgac gacccgcacc ggacccgact atccggtggt
    17821 ctcggcgcga gcagccttcg accggctggc cgccagtccc gtccccgccc tcggcatggc
    17881 ctgcccggag ccggccgtcg gtcagtcgcc tctgtgcggt ggtccggtga cggtgaccgg
    17941 cgcgaccttc ggcctctcgc tcgagcagac gaccgacggg ccgctgctgg tgccggcatg
    18001 gctgttcgag gtggccgact cccccgaccc cgtcccggtg gtcgcggtcg acccggcctt
    18061 ctggggcagc ccagccgacc cggtgccgcc atcgggcgac cccgacggca gcgggggcag
    18121 tggcagcagc gggggaagcg tcggtggggg cgccggcgtc gaacccgtcc cgccgtccgg
    18181 cgtcgaaccc gtcccgccgt ccggcccgtc cggcccaccg atcagcgagc ccgggccggc
    18241 ggtcactcct cccaccgcgg cggccagcgt cgcgtccgcc acagtgggcg cggacgagcg
    18301 cacggtcgag ctggccttct ggggtgcgcc ggacgtcgac gggccctgcg gcgcggcgta
    18361 cgccgtctcc gtcgcgcagg cgccggacgc agtggtggtc gcggtcaacg agcggccgcg
    18421 cccgatcagt gagagtgcga cctgtccggc aatcgctgcc gaacgtcacc tgaccgtggc
    18481 gctggacgcg ccgctggccc agcgcccgat catcgacgcg gcaacgggaa ccccagtgcc
    18541 gctgcatcgc tgacgcggcg ccgccagcga cccagccggc tcagtggccg gcgccggtcg
    18601 cggtcactgg gtcgcggtca gtggaaggag tcgccgcagg cgcaggaccc gccggcgttg
    18661 gggttgtcga tcgtgaaccc ctgcttctcg atggtgtccg cgaagtcgat cgtggcgccc
    18721 tcgaggtacg gggagctcat ccggtcgacg acgacctcga caccgccgaa gtcgcgcacg
    18781 agatcaccgt cgagggtgcg ctcgtcgaag tacagctggt agatcaggcc ggagcagccg
    18841 cccggctgga cgcccacgcg caggcggagg tcgtcacgac cctcctgctc gaggagcgac
    18901 ttgaccttgg tggcggcgac gtcggtgagg atgacgccgt gggcgggagc ctcggcagcg
    18961 gtgtcggctg cgacctgggt ctcgacctgt tcagtcatcg atgtgcgtcc ttcatgtcgt
    19021 tggtcgtggg tgcacctagg caaaccctcg gtctcgggga aatgttcccc gccctcctag
    19081 ggtacgtcgt cgtcagcggc cgggtgacca ggtggccgca acgcgagccg cacgcgcggc
    19141 cagggtgccc acggggtcgg tgatcgcggc ctccacctgc tcggtggtct cggccacggc
    19201 gtaggtgccg gacagcccga gggccatcgc ctcgcgccgt cccaccatcg tctggccggc
    19261 gatgaccacc gtgggtgtcg cggcctcgag ggcggcccgg gccacgccgg cgaccacctt
    19321 gccgcgcagg ctctgccagt cgagacgccc ctctccggtg acgaccaggt cggcggccga
    19381 caccacggag tcgaagccga cagcggtgag cacctcctcg acgccgctga cccggcgtcc
    19441 gccgagcaac atcaggccgt agccgagccc gccggccgct cctgctccgg gctccttgtc
    19501 caggcgacgg ggccggccgg tgagcaggtc gcgggagctc tggtgggtgc gctggaccac
    19561 ctcggtgaac cgccccagcg cggcctcgag ggcctgggcc agcgccggag aggcgccctt
    19621 ctgcggtgcg aagacggctg aggcgccctg cagccccagc aggggcgagt cgacgtccgt
    19681 ggccgcgagc agctcgatcg ccgcgaagcg gtccttgacg tcctccagcc ccttgagggc
    19741 ggcgtcgggg aggtcggcca gagcgagccc accgcgggcc aggtcgtggg cgctgccggc
    19801 gcccagtgcg gccagcatgc cggcgccgcc gtcgttggtg ccactgccac cgagcccgac
    19861 gacgatgcgc tcggcaccct cgcgcagggc ggcgtcgagc agcagtccca cgccataggt
    19921 gctggtgaca ccggggttgc gctcgtccgc ggcgagcagg tgcaggcccg cgg
//
LOCUS       DATIBB010000177        12631 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_1293846, whole genome shotgun sequence.
ACCESSION   DATIBB010000177 DATIBB010000000
VERSION     DATIBB010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 12631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12631
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1293846"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1536
                     /locus_tag="VLC50_07020"
     CDS             <1..1536
                     /locus_tag="VLC50_07020"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HSF27253.1"
                     /translation="IGGNATKGYSGPDRRGTPAAEPTVRVPWGSRRRASLLILTGVGA
                     VAALAVALSGTPTSTRSLAQLAALVTTLLTVAAAIALLVSSRVGGHAPQALVGVGLMV
                     LALAGLPGLPDPDGLLGLVGLLAAGLALVRALRAPAVDATIRPWRLLASLLAAVAVIA
                     LGLTLLVTLIERAGLVDAAAASARPGVLADLGSAGCAAALAAAFVRRPSALARWQACG
                     LAAGFATLAASELVSVAADRPVLGALAAEVAALGALVLLAVGALSLRQSLAVQRVRAA
                     WLRTQLRQRQALDRHRSEVLHDARSVLAGIRSARSTLTRYTSLLDPAGRAELETAMEA
                     ELARLGRLLDRDKSTAAAPGSYDVVRALEPLVVAARQRGLDVTVTGDRTTADGDPDIL
                     AEIVGNLLDNTLTHAPGARAAILVDDHTSGIRVSVIDDGPGIPSELRDSIFQPGPFGP
                     RPAGAPGHGLGLASAHRLADQQGGGLELIDTTDGAWFVIRLRRAVAPAAPSDGTVRAR
                     DGV"
     gene            1560..2213
                     /locus_tag="VLC50_07025"
     CDS             1560..2213
                     /locus_tag="VLC50_07025"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HSF27254.1"
                     /translation="MLVEDHTLLAQALHLALRAEGLPARVVDPEQRQLGQRLLELAPA
                     TVLLDLDLGGFGPADALIGPLTAHGCQVVVLTGETDRARWGECLLAGATAVLAKSEPL
                     EVIIEAVRASREGRPVMDERDRIAALRASAHQRAVDAERFAPFARLTPREQAVLAALV
                     AGHAVAVIAAESAVSQTTVRTQIRAILTKLGVGSQLQAVAMARQVGWPRETAVRTPG"
     gene            complement(2197..3015)
                     /gene="otsB"
                     /locus_tag="VLC50_07030"
     CDS             complement(2197..3015)
                     /gene="otsB"
                     /locus_tag="VLC50_07030"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015659602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HSF27255.1"
                     /translation="MPAHPQVQAVLRAARAHPDQTLVALDYDGTLAPIVDDPEQARPL
                     PETVAVLTALSARVGLVAVVTGRPAGIAVRLGSLDRLAGRQVVVRGAYGAERWDARTG
                     TYDDPPVPPEVAAARVRLQALVEAAGTGVVLEDKGRALAVHTRRADDPAGAFARLRPV
                     VAEVAAELGLRFEPGRQVLEVRPAGVDKGAVLRELVRETGAQVVVYAGDDLGDEPAFE
                     ALRELRAGGLRTLGVLVASAEVAWPDARRDELVDLEVAGPAGLLDLLGLLSQAS"
     gene            complement(3060..3677)
                     /locus_tag="VLC50_07035"
     CDS             complement(3060..3677)
                     /locus_tag="VLC50_07035"
                     /inference="COORDINATES: protein motif:HMM:NF024791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR C-terminal domain-containing protein"
                     /protein_id="HSF27256.1"
                     /translation="MTNPDLPPRRGAHRAPGRAMATVVPSVLAVALVVAVVGVLYLVV
                     GNRSSTQAGPSVSSTPTVQTSPATPPATSPPVSTPPATPSAPATTATSPGVSPSESGA
                     ATSPPQDGLAGDRPAVVVLNQSGQAGLAGRTADLLRTAGWTVSGTGNFRGTVGTTTVY
                     YPPGQEEAAAALAADLPGTPDRIRPRFSNLSMTRLTVVLTSSYPG"
     gene            complement(3693..3989)
                     /locus_tag="VLC50_07040"
     CDS             complement(3693..3989)
                     /locus_tag="VLC50_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012918877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3263 domain-containing protein"
                     /protein_id="HSF27257.1"
                     /translation="MDTAHAIRTESAPAVAELSERDRAILEFERAWWKYAGAKEQAVR
                     ELFDMSATRYYQVLNALIDRPEALAADPMLVKRLRRMRATRQRARSARRLGFES"
     gene            4179..5498
                     /locus_tag="VLC50_07045"
     CDS             4179..5498
                     /locus_tag="VLC50_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006607282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HSF27258.1"
                     /translation="MVLVARAALFADRADAGRRLAALLKPLAGPDVVVLGLPRGGVPV
                     AAEVARRLQAPLDVIVVRKLGVPYQPELGMGAIGEGEVRILNSALLQSARVGPRALAR
                     VETRERAELDRRTARLRAARRRVPLDGRTALVVDDGLATGFTASAACKVARALGAARV
                     VMAAPVASPEAVQVLRADADEVVCLLVPSEFSAIGQWYDDFSQTSEEEVLALLAEAAA
                     APAAAPSGRDEEVTLALGQVTLAGHLRVPAGSGAVVVFVHGSGSSRASPRNRYVASVL
                     ADAGLATLLLDLLTPEEERERANVFDIELLAARLVEVMAWVRRQADLAGTRVGLFGAS
                     TGAAAALWAAAEPSTEVAAIVSRGGRPDLAEPRLADVRAPTLLIVGGADSPVRELNQA
                     AEAELRCERRLVVVPGATHLFEEPGTLQEVARLAREWFLAHLGTEDD"
     gene            complement(5515..5838)
                     /locus_tag="VLC50_07050"
     CDS             complement(5515..5838)
                     /locus_tag="VLC50_07050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF27259.1"
                     /translation="MVVTVALVGVLLAGAYLWGARHPQVSVHEGMALSMEAGISVEAD
                     GRLYAIPVDVRWQDERGLWHDHGRPECLPPVGIGELGPVRFGTVTVATEPGRWTQTVW
                     VSCGG"
     gene            complement(5929..6001)
                     /locus_tag="VLC50_07055"
     tRNA            complement(5929..6001)
                     /locus_tag="VLC50_07055"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5966..5968),aa:Thr,seq:tgt)
     gene            6154..6627
                     /locus_tag="VLC50_07060"
     CDS             6154..6627
                     /locus_tag="VLC50_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013860977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSF27260.1"
                     /translation="MLKRFRVIPDDKAALGDHTATVRRRILIVDDHDGFRAVARAMLQ
                     AEGFEVVGEASDGGSAMSAAALLQPNIVLLDVHLPDRDGFAVSEELAALPAPPVVVLI
                     SSRELGDVRKRVEASPVAGFIPKDELSASSLTALTGGARNATVADSPRWVRGGQQ"
     gene            6611..7966
                     /locus_tag="VLC50_07065"
     CDS             6611..7966
                     /locus_tag="VLC50_07065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF27261.1"
                     /translation="MAVSSELAAPARGLLITSVDVVAGAVFLGVGVVRWRVARRHAML
                     AYAAGVAWFVGGMLPSLLWVHRPLMLHAALAYPAGRLRGYPARGVVAAAWLTAVVPAL
                     ARDPYASLILAGFVAGVTWREAATAPLGSGRAARRAAAPVVLLAAALAVPATARLFSP
                     MLLGSRVPAVVYACLIACSGLAMVTGRATPQREIDVVIELVQNDPARTLARLRREAGI
                     RGEAGTRAVLVAAIELLETNLALHADLAEKVAEVRASRRRLVEAALHERQRLERQLSD
                     GAVRYLNKLEVELNRLRCSSDDLVADIAGRCFQEAVLTRDDLGQIARGLHPRVLTERG
                     LPGALSDLAARSPIPTTVAFPKQRFPELVESTVWYVCAEALANVVKHSAATSAHLDVH
                     VETSVLIVSVRDDGVGGALLSPAGGLAGLADRLAVLDGQIRVKSPPHGGTLVTVQVPV
                     P"
     gene            7963..9648
                     /locus_tag="VLC50_07070"
     CDS             7963..9648
                     /locus_tag="VLC50_07070"
                     /inference="COORDINATES: protein motif:HMM:NF019350.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HSF27262.1"
                     /translation="MNGTRHLVWPVGLVGAALVVATAMDSPLTTYGGSDRRLLWLETA
                     AALALLVVGALHSLGLALLGALWLLPEAAGWITGPVAVRTAADATNHALAAGALVIAV
                     LIVARETRVPRPSLTAALVGGSVAVVARLVMVDPFLDPQCWRTCAPNPWLLVDLPATG
                     RSLERIGLAAMAAVAVVLAFSAFRGIESRRALPATALGLVAELLITDLTRQVVAEDGQ
                     STTYVVLFVVTQAGALGLAAVVGLERQREWRLARELAALAESMLSWPAPSTLAAELGR
                     AVHDPNLRIDYWSDRRGAYFDTDGRPVSPPAQGALRTSVARGGRPVARFVHSPAVDGR
                     GLERALGAALRLVLENEALRAATMAELYEVHASRARIVARAADERKRLERNLHDGAQQ
                     RVAALSLLVRILRNRAREGPEVVAADRAALLTRAALGELRRVARGIYPAVLVDAGLPG
                     ALLDLAQSSSDVAVSVEELTPTRYHGAVEATAYLVTVAALSDARRRGADELKVSAAEH
                     GGRLMVDLRDDRPPSGKHEAESLLDHVRAQSGSLQIRHDGHSTHVTLELPCAS"
     gene            9636..10295
                     /locus_tag="VLC50_07075"
     CDS             9636..10295
                     /locus_tag="VLC50_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013838276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HSF27263.1"
                     /translation="MRVVIADDAALIRAGIAALLQESGIETVGAASDGDGLLDLVAST
                     LPDAAVVDIRMPPTHTDEGILAASRIREEYPSVAVLVLSQYLEASYAMRLIEENATGA
                     GYLLKDRVADGVMLTDALRRVTAGECVVDQMIVAQLLTRARRHSPLDDLSAREREVLG
                     LMAEGRSNGAICELLVLSPRTVESHVASIFVKLGLQGASDGHRRVLAVLAHLRGDPGP
                     H"
     gene            10379..11872
                     /locus_tag="VLC50_07080"
     CDS             10379..11872
                     /locus_tag="VLC50_07080"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF27264.1"
                     /translation="MKSKVLAVQAAAVAVAVLLAACTASDADKSGGPREPIQLVVANN
                     DGNLEGVPALRHFLERATALSEGRLSFRVVSFWQGGNDETHVIKDVASGKADLGWAGT
                     RAFDLVGVDAFRAFHAPLLISSYAAEAAVVRDMAAGLLPSLHSIGLTGLALVADELRH
                     PAGVLNPLLSPKDFHGLTFGTVASGAQSDGIRALGATPSVKPIWQAAASGQLGGFETM
                     WWTYTANGFPSVAPFIAANINLWPRSWVLFANPSTLSGLSDQARGWLTRAGNETSQWS
                     TGHAADAEAVEIARACRQGARIAMATQGQLAELRSAFEPVYAALRSDPVQGKTLEQIE
                     GLVAQASPDRPVDIPDGCAAVSEAEPSTPPSVRVLSGPGRPGRLPEGVYRYRISADEL
                     RAKGLSEADVRLNAGVFTWTLRGGSWSLKQEPVFASATGTSCGGFYDVSGGSASFTTT
                     TVLEVGSCSPPTWTAQWKVEDGELTWSEVSEADLVPVFAPRPWKRIA"
     gene            12197..12586
                     /locus_tag="VLC50_07085"
     CDS             12197..12586
                     /locus_tag="VLC50_07085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF27265.1"
                     /translation="MTHQLEPKDSFRAQAPRAAVAAPSAEKADPPEHQHRKYLIAFVI
                     VLMVLVGGAIIATFTVAQSGTKPSAAPSQQRLGLASAGASSVAFQVGGASTGPTKANQ
                     LLSSTGAPMDPVSPLRTGPLAVHGILP"
BASE COUNT         1661 a         4516 c         4541 g         1913 t
ORIGIN      
        1 atcgggggaa atgcaacaaa gggctactcg ggcccagacc ggcgcggcac gccggccgcc
       61 gagcccaccg tgcgcgtgcc gtggggctcg cgtcgccgcg ccagcctgct catcctcacc
      121 ggggtcggcg ccgtggccgc gctggccgtc gcgctctcgg gcacgcccac gtcgacccgc
      181 agcctcgccc agctggccgc gctggtcacg acgctgctca ccgtggcggc cgcgatcgcg
      241 ctgctggtct cctcccgggt cggcggacac gccccgcagg cgctggtcgg cgtgggcctg
      301 atggtccttg ccctcgccgg gctgcccggg ctgcccgacc ccgacgggct gctcggcctg
      361 gtcggcctgc tggccgccgg gctggcgctg gtccgggcgc tgcgggcccc cgcggtcgat
      421 gccacgatcc ggccgtggcg actgctcgcc agcctgctcg ccgcggtcgc cgtgatcgcg
      481 ctcgggctga ccctgctggt cacgctcatc gagcgcgccg ggctggtcga tgccgccgcg
      541 gcctccgccc ggccaggcgt gctggcggac ctcgggtcgg ccggctgcgc ggcggcgctg
      601 gccgcggcgt tcgtgcgccg gccgtcggcg ctggcgcgct ggcaggcatg cgggctggcc
      661 gccggctttg cgacgctggc cgcctccgag ctggtctcgg tggccgccga ccggcccgtg
      721 ctgggcgctc tggcggccga ggtcgcggcg ctgggtgcgc tagtgctgct ggccgtcggg
      781 gccctgagcc tgcgtcagtc gctggcggtg cagcgcgtgc gggcggcgtg gctgcgcacc
      841 cagctgcgcc agcgccaggc gctcgaccgg caccgcagcg aggtgctgca cgacgcccgc
      901 tcggtgctgg ccggcatcag gtcggcgcgc agcaccctca cccgctacac ctcgctgctc
      961 gacccggccg gacgggccga gctcgagacc gcgatggagg ccgagctggc ccgactgggc
     1021 cggttgctcg accgcgacaa gagcacggcg gccgcccctg ggtcctacga cgtcgtgcgg
     1081 gccctcgagc cgctcgtcgt ggccgcccgc cagcgcgggc tcgacgtcac ggtgaccggc
     1141 gaccggacga ccgccgacgg cgaccccgac atcctcgccg agatcgtcgg caacctgctc
     1201 gacaacacgc tcacccacgc cccgggtgcc cgggcggcga tcctcgtcga cgaccacacc
     1261 agcggcatcc gggtcagcgt gatcgacgac gggcccggca tccccagcga gctgcgtgac
     1321 tcgatcttcc agcccggccc cttcggaccg cggccggcgg gcgcgccggg tcacggcctc
     1381 gggctggcct cggcccatcg gctggccgac cagcaggggg gtgggctcga gctcatcgac
     1441 accaccgacg gggcctggtt cgtcatccgg ctgcgtcggg cggtcgcgcc cgccgccccc
     1501 tccgacggga cggtccgggc acgggacggg gtctgaccac gtccggcggc acgccaatcc
     1561 tgcttgtcga ggaccacacg ctgctcgcgc aggccctgca cctggcgctg cgcgccgagg
     1621 ggctgcccgc ccgggtcgtc gaccccgagc agcgtcagct cggccagcgc ctgctcgagc
     1681 tggccccggc gaccgtgctg ctcgacctcg acctgggcgg gttcggaccg gccgacgcgc
     1741 tcatcggccc gttgacggcc cacggctgcc aggtcgtcgt gctcaccggc gagaccgacc
     1801 gggcgcgctg gggcgagtgc ctgctggcgg gcgcgaccgc cgtgctggcc aagtccgagc
     1861 cgcttgaggt gatcattgag gccgtccggg cgagccggga gggccggccg gtgatggacg
     1921 agcgtgaccg gatcgcggcg ctgcgggcca gcgcgcacca gcgggccgtg gacgccgagc
     1981 ggttcgcgcc gttcgcccgg ctgacgcctc gggagcaggc ggtactggcc gcgctggtgg
     2041 ccggccacgc ggtcgcggtc atcgccgccg agtcggcggt gtcgcagacc accgtgcgca
     2101 cccagatccg cgccatcctc accaagctcg gcgtcgggtc gcagctgcag gcggtcgcga
     2161 tggcgcgcca ggtcgggtgg ccgcgcgaga ccgcggtcag gacgcctggc tgagcagccc
     2221 gagcaggtcg agcaggcccg ccgggccggc cacctcgagg tcgaccagct cgtcgcgccg
     2281 ggcgtcgggc caggcgacct cggccgaggc caccaggacg ccgagcgtgc ggaggccgcc
     2341 ggcccgcagc tcgcgcagcg cctcgaacgc cggctcgtcg ccgaggtcgt cgccggcgta
     2401 gaccacgacc tgcgcgccgg tctcgcgcac gagctcgcgc agcaccgccc ccttgtccac
     2461 cccggccggg cgcacctcga gcacctgccg ccccggctcg aaccgcagcc cgagctcggc
     2521 ggccacctcg gccacgaccg ggcgcagccg ggcgaaggcg ccggccgggt cgtcggcccg
     2581 ccgggtgtgc acggccagtg cccggccctt gtcctcgagc accacgccgg tgccggcggc
     2641 ctcgaccagc gcctgcagcc ggacccgcgc cgccgcgacc tccgggggga ccggcgggtc
     2701 gtcgtacgtc ccggtccggg cgtcccagcg ctcggcgcca taggcacccc gcacgaccac
     2761 ctggcgtccc gcgagccggt cgagcgagcc caggcgtacg gcgatgccgg ccggacgtcc
     2821 ggtgacgacg gccaccaggc ccacccgcgc gctcagcgcg gtcagcacgg cgaccgtctc
     2881 cggcaggggc cgagcctgct cggggtcgtc gacgatcgga gccagcgtgc cgtcgtagtc
     2941 gagcgcgacc agcgtctggt cgggatgcgc ccgggcggcc cggagcaccg cctgcacctg
     3001 cgggtgggca ggcacgtcgt gccccctcgt cccccccgcg tgcgcgtcca tcgccgtggt
     3061 cagcccggat aggagctcgt gagcacgacg gtgagcctgg tcatggacag gttggagaac
     3121 cgtggccgga tccggtcggg ggtgcccggc aggtcggcgg ccagcgcggc ggcggcctcc
     3181 tcctgaccgg gtgggtagta gacggtcgtg gtgcccaccg tgccccggaa gttgccggtg
     3241 ccgctcaccg tccaaccggc cgtgcgcagc aggtcggcgg tgcgcccggc caacccggcc
     3301 tgcccgctct ggttgagcac gacgacggct ggtcggtcgc cggccaggcc gtcctgcggc
     3361 ggggaggtcg ccgcgcccga ctcactcggc gagacgcccg gggaggtcgc ggtagtggcc
     3421 ggcgcgctcg gggtggcggg cggcgtgctc accggggggc tggtggccgg cggcgtggcc
     3481 gggctggtct ggacggtcgg ggtggagctg acgctgggcc cggcctgggt cgagctccgg
     3541 ttgccgacca cgagatagag gaccccgacg acggccacga cgagggccac cgcgagcacg
     3601 gagggcacga cggtcgccat cgcgcggccg ggggcgcggt gagcaccccg ccgcggcggc
     3661 aggtccgggt tggtcacggc atcagaccgg gatcaggact cgaatccgag ccggcgggcc
     3721 gaacgggccc gctgccgggt ggcccgcatc cggcgcagcc gcttgaccag catcgggtcg
     3781 gcggccaggg cctcggggcg gtcgatcagg gcgttgagca cctggtagta gcgggtcgcg
     3841 gacatgtcga acagctcgcg gacggcctgc tccttggcgc cggcgtactt ccaccaggcc
     3901 cgttcgaact cgaggatcgc gcggtcgcgc tcggacagct ccgcgacggc aggggcactc
     3961 tcggtccgga tggcgtgggc agtgtccata acgctccctg ctctcccctg tggctgtcgg
     4021 ccgccatcgt agggcgagaa caacatcggt gtcattcgac catccggttc gggacggcca
     4081 tcggtgacct taggccctgt cccccgttcg ggcctagcca ttgagtcgca gcagatgggg
     4141 gcgccgatcg tgacccgccc accgggcatc gacgggaggt ggtcctggtc gccagagccg
     4201 cgctcttcgc cgaccgggcc gatgccggtc gccggctcgc cgcgctgctg aagccgctgg
     4261 ccggacccga cgtcgtggtg ctcgggctgc cccgcggcgg ggtgccggtg gcggccgagg
     4321 tggcccgccg actgcaggcg ccgctcgacg tgatcgtcgt acgcaagctg ggggtgccct
     4381 accagccgga gctcgggatg ggggcgatcg gcgagggcga ggtccgcatc ctcaactcgg
     4441 cgctgctgca gtccgcccgg gtcggcccgc gcgcgctggc ccgggtcgag acgcgcgagc
     4501 gtgccgagct cgaccggcgc accgcccggc tgcgggccgc ccgtcggcgg gtcccgctcg
     4561 acgggcgcac cgcgctggtc gtcgacgacg ggctggccac cgggttcacc gccagcgcgg
     4621 cgtgcaaggt cgcccgggcg ctgggggcgg cccgggtcgt catggccgcc ccggtggcct
     4681 cgccggaggc ggtgcaggtg ctgcgcgcgg acgccgacga ggtggtctgc ctgctcgtgc
     4741 cgtcggagtt ctcggcgatc ggccagtggt acgacgactt cagccagacc tccgaggagg
     4801 aggtgctcgc gctgctcgcc gaggccgccg cggcgccggc cgccgcgccg tcgggccgcg
     4861 acgaggaggt gacgctggcc ctcggccagg tcacgctggc cgggcacctg cgcgtcccgg
     4921 ccggctcggg cgcggtagtc gtgttcgtgc acggcagcgg cagcagccgg gccagcccgc
     4981 gcaaccgcta cgtcgcctcg gtgctcgccg acgccgggct ggccaccctg ctcctcgacc
     5041 tgctcacccc cgaggaggag cgcgagcggg ccaacgtctt cgacatcgag ctgctcgcgg
     5101 cccggctggt cgaggtgatg gcgtgggtac gtcggcaggc cgacctggcc gggacccggg
     5161 tggggctgtt cggggcgagc accggagccg cggccgcgct gtgggcggcc gccgagccgt
     5221 caacggaagt cgcggcgatc gtctcccgcg gcgggcgccc ggatctggcc gaaccgcggc
     5281 tggccgacgt ccgagccccg acgctgctga tcgtgggtgg ggccgactcc cctgtgcgtg
     5341 agctcaacca ggcggccgag gccgagctgc ggtgcgagcg ccggctcgtc gtcgtacccg
     5401 gtgccactca tctgttcgag gagcccggca cgctccagga ggtcgcccgg ctggcccgcg
     5461 aatggttcct cgcgcacctg ggcaccgagg acgactaggt ggcgcagggc gccatcaccc
     5521 gccgcaggag acccagaccg tctgcgtcca cctgcccggc tcggtcgcga cggtcaccgt
     5581 gccgaagcgc accgggccga gctcgccgat gccgaccggg ggcaaacact cgggtcgtcc
     5641 gtggtcatgc cagagacccc gctcgtcctg ccaccgcacg tcgaccggga tcgcatacaa
     5701 ccggccatcg gcctccaccg agatcccggc ttccatggac agcgccatgc cctcgtgcac
     5761 ggagacctgt ggatgacgtg ctccccagag gtaggccccg gccagcagca ccccgacgag
     5821 cgcgacggtg acgaccacat ctcgtgacca cctgcccgcc agcacggcca cagcgcgcgg
     5881 cgcctccccc atagcccccc caaaggtgac ccggtcagag ccagtcggag ccgacgaggg
     5941 gacttgaacc cctaaccgcc cgattacaag tcgggtgcgc taccagttgc gccacgtcgg
     6001 caggactcgg ggccatggcc aggcaaatgg gcaaccgcta cccgccgacc cctaaagccg
     6061 tcgcaagcct aaggcattag cgcccggcga cggcgatgcc gggaccttcc gcctcgtcgc
     6121 gcagctggag tcgcgcatct tgactcgggc cccatgctca agcggttccg tgtaatcccg
     6181 gacgacaagg ccgcgctggg cgatcacact gcgactgtgc ggcgacgcat cttgattgtc
     6241 gacgatcacg acggttttcg tgccgtcgca cgagcgatgc tccaggccga ggggttcgag
     6301 gtggtcggcg aggccagcga tggcggcagc gccatgtccg ccgctgcgct gctgcagccg
     6361 aacatcgtcc tgctcgatgt gcatctgccc gatcgggacg gtttcgcagt gtctgaggag
     6421 ctcgctgcgc ttcctgctcc accagtcgtg gtgctcatat ctagtcgcga gctcggcgat
     6481 gttcgtaagc gggtcgaagc cagcccagtg gccggcttca tccccaagga cgaactgtca
     6541 gcctcctcgc tcacggctct taccggggga gctagaaatg cgaccgtggc tgattctcct
     6601 cgctgggttc gtggcggtca gcagtgagct ggcagcaccg gctagaggcc tgttgatcac
     6661 atcggtcgac gtggtcgccg gggctgtctt cctcggcgtc ggggtggtcc gctggcgtgt
     6721 tgcgcggcgt cacgcgatgc tcgcctacgc cgcaggtgtc gcgtggttcg tgggagggat
     6781 gctcccctcg ctgctctggg tccatcggcc gctcatgctg catgccgctc tcgcctatcc
     6841 ggcgggccgg ctacgtggct acccggcgag aggagtggtc gccgcggcct ggctgaccgc
     6901 cgtcgtgccg gcgctggcgc gagatccgta tgcctccctg attctcgccg gtttcgtcgc
     6961 gggcgtcacc tggcgcgagg ctgcaacagc tccgctcggc tctggccggg cagcccggcg
     7021 cgccgcggcc ccggtagttc tgctcgcagc tgcgctggcg gtcccggcga ctgctcggct
     7081 gttctcgccc atgctgctcg gtagccgggt gcccgccgtg gtctatgcct gcctcatcgc
     7141 ctgctccggg ctggcaatgg tgaccggtcg agcgacaccg caacgcgaga tcgacgtggt
     7201 catcgagctc gtgcagaacg accccgcacg gacgctcgcg aggctgcgcc gagaggctgg
     7261 catccgtggt gaggccggga cgcgagccgt cttggttgcc gccatcgagc tgttggagac
     7321 caaccttgcc ctgcacgctg acctggccga gaaggtggcc gaagtccgag cctcccggcg
     7381 gcgactggtc gaggctgccc tgcacgagcg ccagcggctg gagcgccagc tgtccgacgg
     7441 tgcggtgcgc tacctcaaca agctcgaggt cgagctgaac aggctgcgtt gctcctctga
     7501 cgatcttgta gcggatatcg ccggccgttg cttccaggag gcggtcctga cccgcgatga
     7561 tctcgggcag atcgctcggg gactgcaccc aagggttctt accgagcgtg gtctgcccgg
     7621 cgccttgtcc gacctcgcgg cgcgttcacc gatcccgacc acggtggcat tcccgaaaca
     7681 acgcttcccc gagctcgtcg agtcgacagt ttggtacgtg tgcgccgaag cgctggccaa
     7741 cgtggtcaag cactcagccg cgacctccgc gcacctcgat gttcacgtcg agaccagcgt
     7801 cctcatcgtc tcggtgcgcg acgacggtgt gggtggcgcg ctcctgagtc cagccggtgg
     7861 actcgccggc ctggcggacc ggctcgcggt gttggacggg cagatccgtg tcaagagtcc
     7921 gccgcacggc gggactctgg tgacggtcca ggtgcccgtg ccatgaacgg cacacgacac
     7981 ctcgtctggc cggtcggtct tgtcggtgcc gcgctcgtgg tggccaccgc catggacagt
     8041 cctctgacga cctatggcgg ctcagaccga agactgctgt ggctcgagac ggccgcggcc
     8101 ctggcgctct tggttgttgg cgcgcttcac tcgctgggcc tggcgctgct cggtgcgttg
     8161 tggctccttc cggaggccgc tgggtggatc accgggcccg ttgctgtgcg caccgcagcc
     8221 gacgcaacga accatgcgct agccgcgggc gccctggtca tcgctgtgct gatcgtcgcg
     8281 cgggagacgc gggtgccccg cccctcgctc accgccgcgc tcgtcggtgg cagcgtcgcc
     8341 gtggttgccc gactggtgat ggtggacccg ttcctcgacc cccaatgctg gcgaacctgc
     8401 gcccccaacc cttggctgct ggtcgacctg ccggccacgg ggcgctcgct cgaacgaatt
     8461 ggcctggctg ccatggcggc agttgccgtc gtgctggcgt tcagcgcgtt ccgcggcatt
     8521 gagagccgcc gcgcgctgcc ggcgacagcc ctcggcctcg ttgccgagtt gctgatcacc
     8581 gacctgaccc ggcaggtcgt cgccgaggac ggacagtcga cgacgtacgt cgtgctattc
     8641 gttgtcacac aggccggcgc gctcgggctt gcggctgttg tcgggctgga gcgacagcga
     8701 gagtggcgcc tcgcccggga gctggccgcg ctcgcggaga gcatgctgtc ctggccggca
     8761 ccgagcacgc tggcagctga gctgggccgg gcagtgcacg atccgaacct gcgcatcgac
     8821 tactggtcgg atcgccgtgg cgcctacttc gataccgacg ggcggccggt ctctccaccg
     8881 gcccagggcg cactgcgaac ttcagtcgcg cgcggcggcc gtccggtggc ccggttcgtg
     8941 cactcgccgg cggtcgacgg tcggggtctg gagcgtgctc tcggtgccgc gctgcggctg
     9001 gtcctggaga atgaggcact tcgtgcagcg acgatggcgg agctgtatga ggtgcatgcc
     9061 tcgcgcgccc gcatcgtcgc ccgtgctgct gacgagcgga agcgactgga gcgcaatctg
     9121 cacgacggcg cccagcagcg tgtggccgcg ctgtcgcttc tggtacggat cctgcgcaac
     9181 cgtgcacgag aaggcccaga agtcgttgct gcggaccggg cggccttgct gacgcgggcc
     9241 gcgctgggcg agctgcgccg ggtcgcccgg ggcatctatc cggccgtgct cgtcgacgcc
     9301 ggccttccgg gcgctttgct cgacctggcg cagtccagca gcgacgtcgc cgtttccgtg
     9361 gaagagctca ccccgacccg ctatcacgga gccgtggagg caacggccta cctggtgacc
     9421 gtggcagccc tctcggacgc gcgtcggcgc ggcgcggatg agctgaaagt ttcggctgcc
     9481 gagcatgggg gccggctgat ggtggacctg cgtgacgacc ggccgccatc gggtaagcac
     9541 gaggcggaaa gtctgctgga ccacgtgcgt gcgcagtcag ggtcgctgca gatccggcac
     9601 gacggacact ccactcacgt gacgttggag ctgccatgcg cgtcgtgatc gccgacgatg
     9661 cagccttgat ccgagcgggc atcgcggccc tgttgcagga gtccgggatc gagacggttg
     9721 gtgcggccag cgacggcgac gggcttctcg acctcgtcgc atcgacgctt ccggacgccg
     9781 cagtcgtcga tatccggatg ccgccgacgc acaccgacga gggcattctc gccgccagca
     9841 ggatccggga agagtatccg agcgtcgctg tgctcgtact ctcccaatac ctcgaggcgt
     9901 cctatgcgat gcggctcatc gaggagaacg cgaccggcgc tggttacttg ctcaaggacc
     9961 gggtcgccga cggcgtgatg ctcaccgatg cgttgcgcag ggtcacggcc ggcgagtgcg
    10021 tcgtcgacca gatgatcgtg gcccagctgc tgacccgcgc ccggcggcac agcccactcg
    10081 acgacctgtc ggcccgggag cgcgaagtgc tcgggctgat ggccgaaggg cgttcaaacg
    10141 gtgcgatctg cgagctgctc gtgctcagcc cgcggaccgt cgagagccac gtcgcgagca
    10201 tcttcgtcaa gctcggactc caaggagcat ccgatggcca ccgtcgggtg ctggcggtcc
    10261 tggctcatct acgcggcgat ccgggtccgc actgaccctc cgggcacgtc ccgatgtgcc
    10321 gtcccggtcg cgagtctgag cctttggggc aggggtactc ctcgagaagg agacggccat
    10381 gaagagcaag gtattggcgg tgcaagctgc ggctgtcgcc gtggccgtcc tgctggccgc
    10441 ctgcaccgcc tcggacgctg acaagtcggg cggcccgcgg gaaccaatcc agctggtcgt
    10501 ggccaacaac gacgggaatc tcgagggcgt ccccgccttg aggcacttcc ttgagcgagc
    10561 tacggctctc tcggagggcc ggttgagctt ccgggtcgtg tccttctggc agggcggcaa
    10621 cgacgaaacg catgtcatca aggatgtggc gtccggcaag gcggatctcg ggtgggcggg
    10681 cacgcgcgcc ttcgacctgg taggagtcga cgcattccga gcctttcacg cgccgctgct
    10741 tatcagctcg tacgccgccg aggcggccgt ggtgcgcgac atggcggcag gccttctgcc
    10801 ctccctgcac tcgatcggtc tcaccgggct tgccctcgtc gcagacgagt tgcgacaccc
    10861 ggccggtgtc ttgaatccgc tcctgtctcc gaaagatttt catggcctca cgtttgggac
    10921 ggtcgcgtct ggtgcgcagt ccgacgggat cagggcactt ggtgcgacgc catctgtgaa
    10981 gccgatttgg caggccgcgg ccagtggcca gctgggcggg ttcgagacga tgtggtggac
    11041 ctacacagcc aacggattcc ccagcgttgc accgttcatc gcggccaaca tcaatctctg
    11101 gccgcgctcc tgggtgcttt tcgcaaaccc cagcacgctg tccgggcttt ccgatcaggc
    11161 tcgtggctgg ctcacgcgag cgggcaacga gacatcgcag tggtcaaccg ggcatgctgc
    11221 ggacgcagag gcggtggaga ttgctcgcgc ctgccgccag ggcgctcgca tcgcgatggc
    11281 gactcaaggg cagcttgccg agttgcgttc ggcgttcgag cccgtctacg ccgcgttgcg
    11341 atccgacccg gtgcagggca agaccctgga acagattgaa ggcctggtcg cacaggcatc
    11401 tcccgaccgg ccggtcgaca ttcccgacgg gtgcgctgct gtgtctgaag ccgagccgtc
    11461 cacgccgccg tccgtgcgtg tcctctctgg acccggccgt ccgggtcggt tgcccgaggg
    11521 ggtctaccgc taccgcatca gcgcggacga gctgcgcgcg aagggcctct ccgaagcaga
    11581 cgtccgtctg aacgctggag tcttcacctg gaccctgcgc ggcggctcct ggagcctaaa
    11641 gcaggagccc gtgttcgcct ccgcgaccgg cacgagctgc gggggcttct acgacgtgag
    11701 cggaggctcg gcgagcttca ccacgacgac ggtcctcgag gtcggctcct gctcccctcc
    11761 gacgtggacg gcgcaatgga aggtcgagga cggtgagctg acatggtccg aggtgagcga
    11821 ggcagacctc gttcccgtct ttgcgccacg accttggaaa cgcatcgcct gacgagtgtt
    11881 tttcctgcct ccccgctgta ggcaaccgac gaacaccgcc agcactggcc caagattgcc
    11941 aggccaatga ccgctgcgcc cttcacggca cgggaaggtc gcaagcatcc cttacgcgca
    12001 tgcgcttccc gcttgtgaac cgcagggtga acaaccccta cgggaatccc gcagacacga
    12061 agccgtgcta gcccggatgc ggcctctgct tgacggccat agcgtcgaaa gacctagcgg
    12121 acccagcgag aggaatgcat ggcgaccatc gctctctcga gatcaatcgc actggagtga
    12181 cgaggaggca acagccatga ctcaccagct cgagcccaag gacagcttcc gtgcccaggc
    12241 acctcgcgca gcagtcgctg cgccgtcggc cgagaaagcc gatccacctg agcaccagca
    12301 ccggaagtat ctgatcgcgt tcgtgatcgt cctgatggtc ctggtcgggg gcgccatcat
    12361 tgcgacgttc actgtcgccc agagcggcac caagccgtcg gccgcgccga gccagcagcg
    12421 gctcgggctt gccagcgccg gcgcgtcctc ggtcgcattt caggtcggcg gtgcgagcac
    12481 cggcccgacc aaagccaacc agctgttgtc gagcaccggc gcaccgatgg atccggtgtc
    12541 acctctcaga accggtcctt tggccgtgca cggaattctg ccctgacccc aggctcccag
    12601 atcaaagcgg ccaccgacct gccgatctac a
//
LOCUS       DATIBB010000178         6739 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_1821499, whole genome shotgun sequence.
ACCESSION   DATIBB010000178 DATIBB010000000
VERSION     DATIBB010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 6739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6739)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6739
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1821499"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..264
                     /locus_tag="VLC50_07090"
     CDS             <1..264
                     /locus_tag="VLC50_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018598941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric-oxide reductase large subunit"
                     /protein_id="HSF27266.1"
                     /translation="SEGYFEARSLGYITSPGNSVLEWMRLPGDVVFIVGGALPFVWIC
                     WLAVRHRVDRVTDRQRDDALFSDEGPVDAAGESTPVGSGRDPM"
     gene            261..896
                     /locus_tag="VLC50_07095"
     CDS             261..896
                     /locus_tag="VLC50_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003882177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF27267.1"
                     /translation="MSILREVDLTAWLTASYAVFLVVVAHGFDRLARVVARSSERWRS
                     GGFVYHEDHDAWLCPQDQMLWPVSFDAKNRVVRYRAKPSICNSCPVKDTCTTSDSGRE
                     ITRDFDPWPHSEAGRFHRGIACTVAALGAALPVATLFVERNPVDVLVLVVTVLLVGVA
                     SLPLVSHFRHSPAGFPDLPIEGESGGPDAVARPTTDRYTTIWGMAPTKETR"
     gene            893..1723
                     /locus_tag="VLC50_07100"
     CDS             893..1723
                     /locus_tag="VLC50_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011725813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HSF27268.1"
                     /translation="MTALVTTVVVVLLLAVLSVLSVRVLREYERGVVFRVGRLRPLYG
                     PGLRLILPAADRLIRVDLRVVTLTIPPQEVITRDNVPARVNAVLLFHVVDPLKAVMTV
                     ENYAVATSQIAQTALRSVLGRADLDTLLAHREDLNEDLRTSIEHQTEPWGVRVTVVEI
                     KDVEIPEAMQRAMAREAEAERERRAKVINARGELQASEELRQAAETLSRSPAAIQLRY
                     LQTLLELGADQNSTVVFPLPIDIIGPFLQKAPELLGRLAAPAPPAAVVDGAGANDHAK
                     "
     gene            complement(1759..2412)
                     /gene="cofC"
                     /locus_tag="VLC50_07105"
     CDS             complement(1759..2412)
                     /gene="cofC"
                     /locus_tag="VLC50_07105"
                     /EC_number="2.7.7.68"
                     /inference="COORDINATES: protein motif:HMM:TIGR03552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043814 - phospholactate
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate guanylyltransferase"
                     /protein_id="HSF27269.1"
                     /translation="MDRTSSERRAFWWVILPLKDTRAGKTRIHLDPPARADLARAMAR
                     DTLTAVLAAQRVDRVVLVCEDLADAALVEHPRLLVHLDQARRGLNPAITAGATLARTL
                     APCAHLAALPADLPALTGPTLDHALESAATHERAFLTDQAGIGTTLLTAAPGHALDPH
                     YGPHSRARHAATGAVELTDPALEPLRSDVDDLDALRCALGRAPALGEAPALGKALCH"
     gene            complement(2415..3104)
                     /gene="npdG"
                     /locus_tag="VLC50_07110"
     CDS             complement(2415..3104)
                     /gene="npdG"
                     /locus_tag="VLC50_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004993279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent F420 reductase"
                     /protein_id="HSF27270.1"
                     /translation="MSVSTIAVIGGTGALGRGLGFRFSAAGHPVVLGSRSADRAATAA
                     AELAGRLTDPAAGVVSGAENAKAAEAADVVVLAVPYAGHAELVSGLAGPLAGKVVISC
                     VNPLGFDKLGPFGLAVPEGSAAEHAAALLPDSQVVAAFHHVSAVTLLGDGAGLADQDV
                     LVCGDDPDARATVVELAAAVTGRAGIEVGPLRLARQLEPLTAVLISINKRYKAHSGVR
                     ISGLPHGTDRH"
     gene            complement(3246..3971)
                     /locus_tag="VLC50_07115"
     CDS             complement(3246..3971)
                     /locus_tag="VLC50_07115"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl homoserine lactonase family protein"
                     /protein_id="HSF27271.1"
                     /translation="MPKRWVRVGGADEPVTIPVLAFLVVAGDRLVLVDSGCTPVVTED
                     PVKAWGRLTSLYHPHIRPDDLVDAAIRRAGFEVGEVTDVVLTHLHFDHAGGLRLLDHR
                     PRVWVQRAEHRWGGCPDGHAAGGYFKQEYDLPGLDLQLLDGDTTIADGVQCVLTAGHT
                     PGHQSVLVRLPSQLACVVGDAAYNRQMLDRRSMPAVAWDVSRYMSTLTRLSTLETFFG
                     AQLLFSHDPAQVDDLPTGTDFLS"
     gene            4123..4890
                     /locus_tag="VLC50_07120"
     CDS             4123..4890
                     /locus_tag="VLC50_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013678208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HSF27272.1"
                     /translation="MDLQLRGKTVLVTGAGQGIGREVGLGFAREGANVAFHYRSSDEG
                     ARAAAAEAAELGVKACAVGGDVADLDDVKAFVAEVREQLGRIDILVNNAAYTSSAPFL
                     EDDVADWRRQVDVTVMGMLQVSQAVLPDLVEGGAGAIVTMAGDSGRVGESRAVATATC
                     RASAYGFTKSVAKEFARNGVRANCVSLGLVRSPSVEQHFLGEATDEFVARVVKAYPLR
                     RLGEMSDVVPAVLMLASPVSGWITGQVLSINGGYATG"
     gene            4911..5669
                     /locus_tag="VLC50_07125"
     CDS             4911..5669
                     /locus_tag="VLC50_07125"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018296229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase family protein"
                     /protein_id="HSF27273.1"
                     /translation="MTASLDLSDSIVVVTGAGSGIGRATACRLAADGAHVVAADINAD
                     GADATVALITAAGHKARAATVDIADADSVAAFHKSVLDTEGRVDVLVNNAGWEDIRPF
                     LDTDRDFWQRLLSINLLGPIAITHAFVTDMVERRSGTIVNVASDAGRVGSSGETVYAG
                     AKGGVIAFTKSLAREMARYGLSVNCVCPGPTDTPAFDRVPDKLKEALIRAIPLRRLAA
                     PDDIANAISFFASPRSSYLTGQVLSVSGGLTMVD"
     gene            5688..>6739
                     /locus_tag="VLC50_07130"
     CDS             5688..>6739
                     /locus_tag="VLC50_07130"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HSF27274.1"
                     /translation="MDTDQRAAFYRREGWWRDSLVVDDLRAHADARRDDVAQVTYSAD
                     GGWSDRLTFGELAEAVDSIATGLLELGVKPGEPVCFQLPNWWHFTAVHLACNRIGAIS
                     CPLIPILRRREVEYVVGLIGARVYIVPSRFRRFDFAALANTVLGSVDCLEHVFTIDGP
                     RGAGGSFERHFLGRSVDAAALDELADRHPSADDIATIQFTSGTTGEPKGVMHTHNSLH
                     ATTKLVPWAFGLDSSDVVMMPSPLAHASGFLYGVLMPVTWGMKVVYQDLWDARRMLEL
                     LDAEGGTWTIGSPPFVMDTIRACRELGRDAAPLRLFSCAGAPIPRYLAEETPQVTGAR
                     LAPNWGLTETGAATITP"
BASE COUNT          974 a         2321 c         2394 g         1050 t
ORIGIN      
        1 tccgagggct acttcgaggc ccgctcgctc ggctacatca ccagcccagg caactctgtg
       61 ctcgagtgga tgcgcctgcc cggcgacgtc gtgttcatcg tcggcggcgc gctgccattc
      121 gtgtggatct gctggctcgc cgtacgtcat cgggtcgatc gggtcaccga ccggcagcgt
      181 gacgacgcgt tgttcagcga cgaggggccg gtcgacgcgg ccggcgagtc caccccggtc
      241 ggttcggggc gggacccgat gtgagcatcc tgcgcgaggt cgacctcacg gcctggctga
      301 ccgcgtcata cgccgtgttc ctggtcgtgg tcgctcacgg gttcgatcgg ctggcccggg
      361 tggtggcgcg cagctccgag cggtggcgca gcggcggctt cgtctaccac gaggatcacg
      421 acgcgtggct gtgcccgcag gaccagatgc tctggccggt gtccttcgac gccaagaacc
      481 gggtggtgcg ctaccgggcc aagccgagca tctgcaacag ctgcccggtc aaggacacct
      541 gtacgacgtc ggacagcggg cgcgagatca cccgcgactt cgacccgtgg ccgcactcgg
      601 aggccggccg gttccaccgc ggcatcgcct gcacggtggc cgccctgggt gcggcgctgc
      661 cggtggccac cctgttcgtc gagcgcaacc cggtcgacgt actcgtcctg gtcgtgaccg
      721 tcctgctggt cggggtggcg agcctgccgc tggtgtcaca cttccgccac tcgccggcgg
      781 gcttccctga cctgccgatc gagggtgagt cgggcggccc ggacgcggtg gcccgcccga
      841 cgaccgaccg ctacaccacc atctggggga tggcccccac gaaggagacc cgatgaccgc
      901 cctcgtgacc acggtcgtgg tcgtcctcct gctggcggtg ctgtcggtgc tcagcgtgcg
      961 ggtgctgcgc gagtacgagc gcggcgtggt gttccgggtc ggacggttgc gcccgctcta
     1021 tgggcccggc ctgcggctga tcctgccggc ggccgaccgg ctgatccggg tcgacctgcg
     1081 ggtggtaacc ctgaccatcc cgccgcagga ggtcatcacc cgcgacaacg tgccggcccg
     1141 ggtcaacgcc gtactcctct tccacgtcgt ggacccgctc aaggcggtga tgacggtgga
     1201 gaactatgcc gtggccacct cgcagatcgc acagaccgcg ctgcgctcgg tgctcggtcg
     1261 ggccgacctg gacacgctgc tggcccaccg cgaggacctc aacgaggacc tgcgcaccag
     1321 catcgagcac cagaccgagc cgtggggcgt gcgggtcacc gtcgtggaga tcaaggacgt
     1381 ggagatcccc gaggcgatgc agcgggcgat ggcccgcgaa gccgaggccg agcgggagcg
     1441 ccgcgccaag gtgatcaacg cgcgcggcga gctgcaggcc tccgaggagc tgcgccaggc
     1501 cgccgagacg ctcagccgca gcccggccgc gatccagctg cgctacctgc agacgttgct
     1561 cgagctgggc gccgaccaga actcgaccgt ggtgttcccg ctgccgatcg acatcatcgg
     1621 cccgttcctg cagaaggccc ccgagctgct cggccgcctc gccgcccccg cgccccccgc
     1681 cgccgtcgtc gacggggccg gcgcgaatga ccacgccaag taggcgtccg tccgcttgac
     1741 aaggcgtgca cgcctcgcct agtgacacaa cgccttgcca agggcggggg cctcgccaag
     1801 ggcgggggcc ctgccaaggg cgcaacggag cgcgtccagg tcgtcgacgt cggagcggag
     1861 cggctcgagc gcggggtcgg tgagctcgac ggcgccggtg gcggcgtgtc gggcgcggga
     1921 gtggggcccg tagtgcgggt cgagggcgtg tccgggggcg gcggtgagca gggtggtgcc
     1981 gatcccggcc tggtcggtga ggaaggcccg ctcgtgggtg gcggccgact ccagggcgtg
     2041 gtcgagggtc ggtccggtca gggcgggcag gtcggccggc agcgcagcga ggtgagcgca
     2101 gggggcgagg gtgcgggcca gggtggcgcc ggcggtgatg gccgggttga gcccacggcg
     2161 ggcctggtcg aggtgcacga gcaggcgggg gtgctcgacc agcgcggcgt cggcgaggtc
     2221 ctcgcagacc agcacgaccc ggtccacccg ctgggcggcc agcacggcgg tgagggtgtc
     2281 gcgagccatc gcgcgagcca ggtcagcgcg ggcgggcggg tcgaggtgga tgcgggtctt
     2341 gccggcccgg gtgtccttca gcggcaagat cacccaccag aaggcccgcc gttcggacga
     2401 ggtgcggtcc atcgtcagtg ccggtcggtc ccgtggggca gcccggagat gcgcaccccg
     2461 gagtgggcct tgtaccgctt gttgatcgag atgagtacgg cggtcagcgg ctcgagctgg
     2521 cgggccagcc gcagcgggcc gacctcgatg ccggcccgcc cggtcaccgc cgcggccagc
     2581 tcgaccaccg tcgcgcgagc gtccgggtcg tcgccgcaga ccagcacgtc ctgatcggcc
     2641 agcccggccc cgtcgccgag cagggtgacc gccgagacgt ggtggaatgc cgcgaccacc
     2701 tgggagtcgg gcagcagtgc ggcggcatgc tcggcggcgc tgccctcggg cacggccagc
     2761 ccgaacgggc cgagcttgtc gaacccgagc gggttgacac agctgatcac gaccttgccg
     2821 gccagcggcc cggccagccc ggagaccagc tcggcgtgcc cggcgtaggg caccgccagc
     2881 accaccacgt ccgcggcctc ggccgccttc gcgttctccg ccccgctgac caccccggcg
     2941 gccgggtcag tcagccgacc cgccagctcc gcagccgccg tcgccgcccg atccgcactg
     3001 cgcgagccca gcaccaccgg gtgccccgca gccgagaacc gaaaccccag acccctgccc
     3061 agtgcgccgg tgccaccgat caccgcgatc gtcgagacgc tcatgctgct ccagtccaac
     3121 gatgacccgc tgccgggccg ccgggtttgg tccggcgagg agggctccgc ccagctcatc
     3181 atccccgggt tcagaggggc agtgccatcg cccccgcgca ctcgtcgagg ctgtcgtggc
     3241 tggtctcagc tgaggaagtc ggtgccggtg ggcaggtcgt ccacctgggc cgggtcgtgg
     3301 ctgaacagca gctgcgcccc gaagaacgtc tccagcgtcg agagccgggt cagcgtcgac
     3361 atgtagcggc tgacgtccca ggcgaccgcc ggcatcgagc gtcggtcgag catctggcgg
     3421 ttgtaggccg catcgcccac cacgcaggcg agctgggagg gcaggcgcac gagcaccgac
     3481 tggtgcccgg gggtgtgccc ggcggtgagc acgcactgca ccccgtcagc aatcgtcgtg
     3541 tcgccgtcga gcagctgcag gtcgaggccg ggtaggtcgt actcctgctt gaaatagcca
     3601 ccggcggcgt gcccatcggg gcagccgccc caccggtgct cggcacgctg cacccacacc
     3661 cgggggcggt ggtcgagcag ccggagcccc ccggcgtggt cgaagtgcag atgcgtgagt
     3721 acgacgtcgg tgacctcacc gacctcgaac ccggccctgc ggatcgcagc gtcgacgagg
     3781 tcgtcggggc ggatgtgcgg gtgatagagc gaggtgagcc tgccccaggc cttgaccggg
     3841 tcctcggtca ctacgggcgt gcagccgctg tcgaccagga ccagccggtc accggccacg
     3901 acgaggaagg ccagcacggg gatggtcacc ggctcgtcgg cgcccccaac gcgcacccac
     3961 cgcttcggca tggtgattcg tccggcccgt aggacgtgaa ctcggtcagc catgacggct
     4021 cccttcgggc ggtccggggc ccattgaaac gtgtgatgca tagtagtata ttagagtttc
     4081 ccgatggccc cggtgccgcg acgagatgac acgaggagcc ccatggattt gcagttgcgc
     4141 ggaaagacgg tgctggtcac cggggcaggt cagggtatcg gccgtgaggt cggtctcggg
     4201 ttcgcccgcg agggtgccaa cgtcgccttt cattatcgca gctcggacga gggcgcacgg
     4261 gctgcggccg ccgaggcggc cgagctgggt gtcaaggcct gtgcggtggg cggcgacgtg
     4321 gccgatctcg acgacgtcaa ggccttcgtg gccgaggtcc gcgagcagct gggccgcatt
     4381 gacattcttg tcaacaacgc cgcctacacg agcagcgcgc cgttcctcga agatgacgtg
     4441 gccgactggc gtcgacaggt cgacgtcacc gtcatgggca tgcttcaggt gagccaggcg
     4501 gtgctgccag acctggtcga gggtggtgcc ggcgcgatcg tcaccatggc cggcgactcc
     4561 ggccgggtcg gcgagagtcg ggccgtggcc accgcgactt gccgcgccag cgcctacggc
     4621 ttcacgaagt cagtcgccaa ggagttcgcc cgcaacgggg tccgcgccaa ctgtgtctct
     4681 cttggactcg tgcggtcccc gtctgtcgag cagcacttcc tcggcgaggc gaccgacgag
     4741 ttcgttgcac gtgtggtcaa ggcctacccg ctgcgacggt tgggtgagat gagtgacgtc
     4801 gtacccgccg tgctcatgct cgcctcgccg gtctccggct ggatcaccgg acaggtgctg
     4861 tcgatcaacg gcggctacgc caccggctga tacagaaggg cctcatcgcc atgactgctt
     4921 cacttgacct atctgattcc attgtcgtgg ttaccggcgc cggctccggc atcggtcgcg
     4981 ccaccgcatg ccgactcgca gcagacggtg cccacgtcgt ggctgccgac atcaatgccg
     5041 acggtgctga tgcgacagtg gcactgatca ctgcggctgg tcacaaggcc cgggccgcca
     5101 cggtcgatat cgctgacgcc gactcggttg cggcatttca caagtcggtg cttgacaccg
     5161 agggccgtgt cgacgtgctc gtcaacaacg cgggttggga ggacattcgt ccgttcctcg
     5221 ataccgatcg cgacttctgg cagagactct tgtcgatcaa cctgctcgga ccgatcgcca
     5281 tcactcacgc attcgtcacc gacatggtgg agcgcaggtc tggcacgatc gtcaacgtcg
     5341 cctccgatgc cggtcgagtt ggcagcagcg gcgagacggt ctacgccggt gcaaagggtg
     5401 gggtcatcgc gttcaccaag tcgttggccc gtgagatggc tcgttacggg ctctcggtca
     5461 actgcgtgtg tcccggcccg accgacaccc ctgccttcga ccgggtgccc gacaagctca
     5521 aggaggccct catccgggcg atcccgttgc gtcggctcgc cgcgcccgac gacattgcca
     5581 acgccatcag ctttttcgct tccccgcgct cgtcctacct cactggtcag gtgctcagcg
     5641 tgagcggcgg cctcaccatg gtcgactgac gaaggactgc gcacacgatg gacaccgacc
     5701 agagagccgc cttctatcgc cgtgagggat ggtggcgtga ctcccttgtc gtcgacgacc
     5761 tgagagcgca cgccgacgca cgacgcgatg acgtcgcaca ggtcacatac tccgccgacg
     5821 gcggatggag cgaccggctc accttcggcg agctggccga ggctgtcgac agcatcgcga
     5881 ccgggctgct cgagctcgga gtgaagcccg gtgagccggt ctgcttccag ttgcccaact
     5941 ggtggcactt caccgcggtg cacctggcct gtaaccggat cggcgccata tcgtgcccgc
     6001 tcatcccgat cttgcgtagg cgtgaggtcg aatatgttgt cgggctgatc ggggcgcggg
     6061 tctacatcgt gccgtcgcgg tttcgcaggt tcgacttcgc ggccctggcc aatacggtgc
     6121 ttggcagcgt cgactgcctc gagcacgtct tcaccattga cggcccgcgg ggagcaggcg
     6181 gttcgtttga gcggcacttc ctcggtcgct cggtcgatgc ggcagccctc gatgagctgg
     6241 ctgatcggca cccgagtgcc gacgacatcg cgacgatcca gttcacttcc ggcaccaccg
     6301 gcgagcccaa gggggtgatg cacacccaca acagcctgca cgcgacgacc aagctggtgc
     6361 cctgggcgtt cggcctggac agctctgacg tggtgatgat gccctcgccg ctggcgcacg
     6421 cctcgggatt cctctatggc gtcctgatgc cggtgacctg gggcatgaag gtcgtctacc
     6481 aagacctgtg ggacgcccgg cggatgctcg agctgctcga cgccgagggc ggcacctgga
     6541 ccatcggttc gccgccgttt gtcatggaca ccatccgggc ctgccgggag ctcggtcgcg
     6601 acgccgcgcc gttgcggctg ttctcctgcg ccggtgcgcc gatcccgcgc tacctcgccg
     6661 aggagacccc ccaggtgacc ggcgcccggc tggcccccaa ctgggggttg accgagaccg
     6721 gcgcggccac gatcacccc
//
LOCUS       DATIBB010000179         2003 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_1821603, whole genome shotgun sequence.
ACCESSION   DATIBB010000179 DATIBB010000000
VERSION     DATIBB010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1821603"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..339)
                     /locus_tag="VLC50_07135"
     CDS             complement(<1..339)
                     /locus_tag="VLC50_07135"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010032607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HSF27275.1"
                     /translation="MAEQLYATLHTSKGDVTVALFPDFAPKTVRNFVELAEGTREWTH
                     PKTRAKTTDKLYDGTIFHRVIPGFMIQGGDPLGTGTGGPGYEFDDEPHPEKTFDRPYL
                     LAMANAGKRGD"
     gene            543..791
                     /locus_tag="VLC50_07140"
     CDS             543..791
                     /locus_tag="VLC50_07140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF27276.1"
                     /translation="MGRRERHQKRTDMHETVESVEPAEAHETSEMHPKKKHRGRRIIG
                     MMAVAGTAAAAGKAMQARRHAQGQPSGQDPTLSGTSSL"
     gene            832..1236
                     /locus_tag="VLC50_07145"
     CDS             832..1236
                     /locus_tag="VLC50_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006603946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HSF27277.1"
                     /translation="MDWALEVVVVPVSDVDRAKRFYAEQVGFTVDHDTPIGKDLRVVQ
                     LTPPGSACSIVIGAGLAEMPPGAVKGLQLVVDDVEAARAVLAAGGVDVTPVRHNEGGT
                     WVDGPGGEWNSFIFFSDPDGNAWTVQQRPPRG"
     gene            1282..1749
                     /locus_tag="VLC50_07150"
     CDS             1282..1749
                     /locus_tag="VLC50_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014690371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HSF27278.1"
                     /translation="MLGRALRDLVTVTRLLTTAESEARHAGRERPGPEDLLLAALSLP
                     DGTAARVFRRFGLDEGAVRTAIATARAEDGGSDGVRGRAGAYRATPAGQAVFRAAVAL
                     TKESTPRSRLLGAHVVAASADLDRGVVARALRRLAIDPATLRTTACAQAREDL"
     gene            complement(1758..1830)
                     /locus_tag="VLC50_07155"
     tRNA            complement(1758..1830)
                     /locus_tag="VLC50_07155"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1795..1797),aa:Ala,seq:tgc)
     gene            complement(1875..1994)
                     /locus_tag="VLC50_07160"
     CDS             complement(1875..1994)
                     /locus_tag="VLC50_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019874431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DLW-39 family protein"
                     /protein_id="HSF27279.1"
                     /translation="MKKLLLTLIAAAGGFLAYRKVAQAKAEQDLWNEATDPLR"
BASE COUNT          275 a          687 c          771 g          270 t
ORIGIN      
        1 gtcgccgcgc ttgccggcgt tggccatcgc cagcaggtag ggccggtcga acgtcttctc
       61 cgggtgcggc tcgtcgtcga actcgtagcc cgggccgccg gtgccggtgc ccagcgggtc
      121 accaccttgg atcatgaagc ccgggatcac ccggtggaag atcgtgccgt cgtagagctt
      181 gtcggtggtc ttggcccgcg tcttggggtg ggtccactcc cgcgtgccct cggccagctc
      241 gacgaagttg cgcaccgtct tgggcgcgaa gtcgggaaag agcgccacgg tgacgtcgcc
      301 cttgctcgtg tgcagggtcg cgtagagctg ctcggccacg atggtcctct ccgattcggt
      361 cggtacggcg tgtgcaggca tcgcgccgac gcggctatgc gtgccggccc atcctcgcac
      421 gccgcccgcg acgcaccacg gcccatgtcg gctcgtcgta gacccgtaag gccctcgacg
      481 tgccgcacga tcgggcgcag ggtgggggcg acgttacggc cctgacgaca tgggaggcga
      541 tcatgggcag gcgcgaacgg caccagaagc ggaccgacat gcacgagacc gtcgagtcgg
      601 tcgagccggc cgaggctcac gagacctccg agatgcaccc caagaagaag caccggggcc
      661 ggcggatcat cggcatgatg gcggtggctg gtaccgcggc cgctgccggc aaggcgatgc
      721 aggcgcgtag gcacgcgcag gggcagccgt cggggcagga cccgacgctg tccggcacct
      781 cctcgctcta gtggcggggc gcccagcgac ccaccgacaa gagggggcgg cgtggactgg
      841 gcgctcgaag tcgtcgtcgt accggtctcg gacgtcgacc gcgccaagcg cttctacgcc
      901 gagcaggtcg gcttcaccgt cgaccacgac accccgatcg gcaaggacct gcgggtggtg
      961 cagctgaccc cgcccggctc ggcctgctcg atcgtcatcg gagccggcct ggccgagatg
     1021 ccgccggggg cggtcaaggg gctgcagctg gtggtcgacg acgtcgaggc cgcccgcgcg
     1081 gtcctggccg cgggcggcgt cgacgtcact ccggtccggc acaacgaggg cggcacctgg
     1141 gtcgacgggc ccggcggcga gtggaactcg ttcatcttct tcagcgaccc cgacggcaac
     1201 gcgtggacgg tgcagcagcg accgccgcgc ggctgatcgg tgcggcgacg tagcacggtc
     1261 ggcgcggggc cgggaggacg gatgctgggg cgggcgctac gcgacctggt cacggtcacg
     1321 cggctgctca ccaccgccga gtccgaggcc cgtcacgccg ggcgcgagcg ccccggcccg
     1381 gaggacctgc tgctcgcggc gctgtccctg cccgacggca ccgcggcccg ggtgttccgc
     1441 cgcttcgggc tggatgaagg agccgtgcgt acggcgatcg cgaccgcgcg cgccgaggat
     1501 ggtggctcgg acggagtccg cggccgggcg ggggcctacc gcgccacccc cgcggggcag
     1561 gcggtgttcc gggcggccgt ggcgctgacc aaggagagca cgccgcggtc gcggctgctc
     1621 ggcgcccacg tggtcgccgc gagcgccgac ctcgaccggg gcgtggtcgc gcgggcgctg
     1681 cggcgcctcg cgatcgaccc ggcgacgctg cgcacgacgg cctgcgcgca ggcgcgcgag
     1741 gatctgtgag acgggcgtgg agacgaaggg actcgaaccc tcaacccccg ccttgcaaag
     1801 gcggtgctct gccagttgag ctacgtcccc gaggggcggg ccaagcctag agcacggggt
     1861 cggcgaggcc ggagtcagcg cagggggtcg gtggcttcgt tccagaggtc ctgctcggcc
     1921 ttggcctggg ccaccttgcg gtaggcgagg aaaccaccgg cggcggcgat cagcgtgagc
     1981 aggagcttct tcacgacggg atc
//
LOCUS       DATIBB010000180        20163 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_244152, whole genome shotgun sequence.
ACCESSION   DATIBB010000180 DATIBB010000000
VERSION     DATIBB010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 20163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20163
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_244152"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..627
                     /locus_tag="VLC50_07165"
     CDS             <1..627
                     /locus_tag="VLC50_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016189265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HSF27280.1"
                     /translation="YLGYVTGLAGTDLAAARRGRMLAGTSLFVAGFTVVFVAYGVLFG
                     GLGKALIEYGDVITRVLGAITVVLGLAFMGLVPGLMREWRVHRRPGLGLAGAPLLGVL
                     FGLGWTPCIGPTLGAVQSLAYDTASAGRGALLSAIYCIGLGLPFLVAALAYRRMLGAF
                     AWVRRHSLWVTRLGGLMLVLIGLLLVTGLWTELTVALRGWVGGFETVL"
     gene            627..2246
                     /locus_tag="VLC50_07170"
     CDS             627..2246
                     /locus_tag="VLC50_07170"
                     /inference="COORDINATES: protein motif:HMM:NF016995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HSF27281.1"
                     /translation="MSADPTPTGAPAAAGLSTAPVPTPDRPGALRPREFARWIWRQLT
                     SMRTALVLLFLLAVAAVPGSLVPQESVSPIRVVDFKRDHPTLGPIYERLGMFDVYTSP
                     WFAAIYLLLIVSLVGCILPRSRAHLRALRATPPATPRNLARLAAYRTWGTDATPEAVL
                     ERSRAVLRKRRFRVAPGDGSVAAEAGYLRETGNLVFHIALLLMLAAVALGSLFGWKGT
                     VLVPEGSSFANTVSEFDSFTKGALVDERAMVPFTLALDKLDVRYQQTGQQAGAPRGFT
                     ADVTFRPDPAMPAQRSRIQVNSPLVVDGTKVFLMGNGYAPRFTVRDAAGTVVFAGAVP
                     FLPQESTTFTSTGVIKVPDIKPEQLGFSALFLPTAKVDPELGPVSVFPDALNPEVFLG
                     AYKGDLGIDAGLPKSVYRLDTDDMTLVANGRLLPGQTLTLPDGLGSITYDGWTRWANF
                     QVADDPGRFPALVAALAAIGGLMLSLFIRRRRVWVRAVAGPGGRTVVEVAGLARSEST
                     ALEDEIASLTDELGGPGESRPDPSTNSTGKE"
     gene            2303..3262
                     /gene="ccsB"
                     /locus_tag="VLC50_07175"
     CDS             2303..3262
                     /gene="ccsB"
                     /locus_tag="VLC50_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020135984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcsB"
                     /protein_id="HSF27282.1"
                     /translation="MVVYTGAMYCYAAELAFGNRSARRVAATERELAAVGAPAAGAPA
                     GLPTADSSAASSYGDEASAPQPLPLGHRMGRIAVSLTVLAFLLHLAAVVTRGASAGRM
                     PWGNMFEFATAAALAVTGVFLVLLARKPVRYLGLFVVGPVLLMLGLAVTVLYTESEQL
                     VPALKSVWLGIHVTAAIVAMGLFTVGFSQTILYLIQARRERVPAAERTPNFMDHFPAA
                     AVLDRAAYRMHAFVFPLWTFAIVAGAIWAENAWGRYWGWDPKEVWSFITWVAYAAYLH
                     ARATAGWRGHKAAYVALLAYGTILFNYLVVNIWLVGFHSYSGV"
     gene            complement(3268..5094)
                     /locus_tag="VLC50_07180"
     CDS             complement(3268..5094)
                     /locus_tag="VLC50_07180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF27283.1"
                     /translation="MSATPDVPTTSAEDLLRRHEPVVRYTSGELFLPSSVEAYLRSAA
                     LFRVADDTTEQLAAPTTLDTTALASIGRAHHGERLWLRFVEAPLGRRAYREWRRRPER
                     VRFVGESGAAAVGLLARLVAALMRLSLLVRGRVPGGYAAAAEQAIAAAGGDQRAHYYG
                     HVTRDGGYLVLQYWFFYAMNDWRSTFGGVNDHEADWEQVTVFAVEHDGTSTPAWVAFS
                     SHDETGADLRRRCDDPDLEWVGLHPVVYAGAGSHSGSCLPGDYLITVSPPLPGWLEAT
                     RRRLMRVIPWSSPDPDGIGIPFIDYHRGDGRAVGPGHEQQWTVEVIDDTTPWVRDYTG
                     LWGLDTHDPLGGERAPAGPRYERDGRVRASWSQPVAWAALDSEAPTSAEAAAALGRRA
                     TDLRQRVAELDVELAHARDELRGARALDQTQQRSPRAPGADVRAMAARVAALREEQHR
                     LCRSLEATVVATERADEAAGRAGRSRVSTTDPVHAHLRHRPLPISKTPVNRSWLARVW
                     TSASASVLFAALGLVLLSSSSDVLGVSLVVVAVMLVIEAVVHRRLLALIISLVTLALV
                     VIAAIAAVALVVGNARVGFGILLLLAAGFMAVQTVVDALRRR"
     gene            complement(5162..5626)
                     /locus_tag="VLC50_07185"
     CDS             complement(5162..5626)
                     /locus_tag="VLC50_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014178653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLD nuclease N-terminal domain-containing
                     protein"
                     /protein_id="HSF27284.1"
                     /translation="MEVGVSRVLPILLSIGLTVYCLIDALQTDEQAMRNLPKPFWLLL
                     ILLFPIVGPLAWLFAGRPANDAQAGTRSTQGWLGGGSAGPLRRAPRATQRAVAPDDDP
                     EFLASLRGLDDEHQALLEKWEADLRERERRLREDGSPDNGEPPSGDGSPPSP"
     gene            complement(5716..5841)
                     /locus_tag="VLC50_07190"
     CDS             complement(5716..5841)
                     /locus_tag="VLC50_07190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF27285.1"
                     /translation="MITLELARVAVRDLQRAAEAERLNSVRRPRARRRLRDLWHH"
     gene            5984..6430
                     /locus_tag="VLC50_07195"
     CDS             5984..6430
                     /locus_tag="VLC50_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020388403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HSF27286.1"
                     /translation="MDAIDRRLVEELRANGRASYAELSRLVGLSAPAVQDRVRRLETT
                     GVITGYHAAVDPQALGLGVSALVSVYQSDSTDQDDVAARLAEVPEVESCWFVAGDEAF
                     VLTVRARDIAALETVLGTLRRLKGVARTRTSVVLSTRWEGRVATTG"
     gene            6501..6779
                     /locus_tag="VLC50_07200"
     CDS             6501..6779
                     /locus_tag="VLC50_07200"
                     /inference="COORDINATES: protein motif:HMM:NF025378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4229 domain-containing protein"
                     /protein_id="HSF27287.1"
                     /translation="MAAPPALRYSLLRVTLFVAALALWFGLGLRGFWLLIAAVLMSGV
                     VSYFLLTEPRAAMSERLSARFSRLGRRIDERTRAEDDDEHPDDGQRNV"
     gene            complement(6764..8020)
                     /locus_tag="VLC50_07205"
     CDS             complement(6764..8020)
                     /locus_tag="VLC50_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018587978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid deaminase/aldolase"
                     /protein_id="HSF27288.1"
                     /translation="MGSPRADTEALQTLRARLDHATAELDPPLAVVDLTAFDANADAL
                     VARAGGRPVRVASKSVRSRALLIRALARPGFHGVLAFTLPEALWLVAAGVTDDVVVAY
                     PTADRAALRALAADERALAAVAIMVDDLAQLDLVDAAMGAGHPPVRVCLELDVAWRPL
                     EPRGRLGQRVHVGARRSPVRSPEQARGLAQALVRRPGFRLVGVMAYEAQVAGVPDAPR
                     GGPAAAVRGFAVRRMQAASVAELHERRAAMIDAVRAVATAPLELVNGGGTGSLETSAA
                     DPTLTELAAGSGLYGPALFDGYSRFRPRPAALFALPVVRRPGPGWVTVLGGGYLASGP
                     GQRDRLPTPYLPAGLRLSATEGAGEVQTPLHGPGADRLRVGDRVWLRHAKAGELAERF
                     ALLHLVDGDRVVDAAPTYRGEGQTFL"
     gene            8054..9370
                     /locus_tag="VLC50_07210"
     CDS             8054..9370
                     /locus_tag="VLC50_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005161710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003885 - D-arabinono-1,4-lactone oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-arabinono-1,4-lactone oxidase"
                     /protein_id="HSF27289.1"
                     /translation="MSPRWTNWSGTVRTQMVRQEHPRDVEAVAAAVRAAGRDGLRVKP
                     VGAGHSFTGAAATDGVQLVLDGLTGIVHADLDSGLVTVRAGTRLHQLGRALDALGLAM
                     TNLGDIDAQTIAGAISTGTHGTGGALGSLSSQVRAVELVTADGAVLTASTESAPEVFE
                     AARLGLGALGVLTSVTLACEPAFLLRAEEAPMPLDAVLDELDGLVQGNDHVEFFWWPH
                     TRSTTVKRLNRLPADAAPAPRHPWRAWFDDRFMANTVFGAATALGRRCPAAVPLVNRA
                     ATTLAGSRSYADASYRVLATPRLVRFAESEWALPREALPEVLGQLEAVTRRLEHPVAF
                     PVEVRFGPAENVWLSPAHGRATVYVAVHQALGMPRERWFADVAALALAAGGRPHWGKL
                     HPLDAARLCALYPRFDDFVAVRDRLDPRGMFANAYLDRVLGPASGS"
     gene            complement(9367..10482)
                     /locus_tag="VLC50_07215"
     CDS             complement(9367..10482)
                     /locus_tag="VLC50_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014059044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent alcohol dehydrogenase"
                     /protein_id="HSF27290.1"
                     /translation="MKAAVLHRPGDPLVIEDVELTEPGPGDVLVRVEAAGVCHSDLHY
                     INGDLVAPLPAVLGHEGTGVVEQVGPGVTIVKPGDTVITTWRPRCGDCEQCSTGRPGL
                     CALGKAQAVSGGLWDGRSRLRLRGEPIHHLMGVSCFAQRCIVSDRSVIPISGDVPPQI
                     AAIMGCAVITGVGAALNQMRGATGEAAVVIGAGGVGLSAVMGLRVIGANPIIVVDTVE
                     TRLRRALELGATHVVNAATQEVRAEIAAIASPAPRWVLNAVGAPATLEQAVGIAGVGA
                     TIVVVGLTKAGATFQVPVNELVQQEKRIVGSLYGSSNMLFEIPRLLRLYRDGRLPLDA
                     LVGQQCRLDQINDAFAAMTDGAIGRAVVRPQLEDALG"
     gene            10552..12024
                     /locus_tag="VLC50_07220"
     CDS             10552..12024
                     /locus_tag="VLC50_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019969919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase (NADP(+))"
                     /protein_id="HSF27291.1"
                     /translation="MQSGASGPTAAVGEVVEAASEAFDSWGTSTLDERATALRAVAAA
                     LDASADELVPLAGAETHLGLDRLRGELRRTTFQLRFFADEVRTGRHLEATIDHADEHW
                     GMGPRPDLRCVAAPLGPVLVFAASNFPFAFSVAGGDTASALAAGCPVVVKAHEGHLEL
                     SRATARVVRDALAGVGAPPGLFDLVVGVEAGRDALVDPRVKAAAFTGSIAGGRALFDL
                     AMARPEPIPFFGEMGSVNPTFVTRAAAAARAEQIVAGFLDSFTKGAGQFCTKPGILLV
                     PTGSQLTERLAAALSARLSVPAAPLLNQRIHDGFVRGLAALQAREGIRALVIGDDALA
                     DPPTPTLLLTTIERILAAPDEVITEVFGPTALVVEYERESQLLDLARLLPGQLSASIH
                     GVDDDPIAPQLIQLLAQRAGRVLWGQWPTGVSVTYAQQHGGPYPATTAASTTSVGAAA
                     IRRFLRPVAYQGVPDLLLPPSLQEANPLGVPRRVDGNSAG"
     gene            complement(12021..12833)
                     /locus_tag="VLC50_07225"
     CDS             complement(12021..12833)
                     /locus_tag="VLC50_07225"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HSF27292.1"
                     /translation="MGEPTAAQRWRAELAAWAIDPAILAAAPEPPYRLPPAHFQAEHR
                     GAFDPGPQSSRDRALEALPAGGAVLDVGCGAGAASLALAPPAQRLVGVDELPDMLAAF
                     ARAALEHEVAAETVEGSWPAVAGEVGTADVVVCHHVLYNVADAVPFVRALTEHARRRV
                     VVELTAAHPWVGLGPLWARAHGQPRPSGPTAELGAALVHEAGYDVQVETGRRRTRAAP
                     WDERVATMRRRLCLPIEREAEVAAWMREAGFTAGDPAHQDIVTLWWEPSSSA"
     gene            complement(12864..13733)
                     /locus_tag="VLC50_07230"
     CDS             complement(12864..13733)
                     /locus_tag="VLC50_07230"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019875087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046428 - 1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HSF27293.1"
                     /translation="MARTSEWIEGARPRTLPAAVSPVLAGTGAAVAVDGAVWWKALLA
                     LMVALALQVGVNYANDYSDGVRGTDAVRVGPLRLVGSGTASPGAVKAAALGSFALAAV
                     AGFVLAATSAWWLLLVGVAAIAAAWTYTGGAHPYGYHALGEVGVFVFFGLVAVLGTTY
                     VQAGTITWPALAAAVGVGAYACAILVANNLRDIPSDAAAGKRTLAVVLGDAASRRLYV
                     GLVVLAQLVALAVAVRHPWTVLTLLALAVVARAVATVRAGVVGPALIPVLRDTGRAEL
                     AYAALFALALALP"
     gene            13829..14245
                     /locus_tag="VLC50_07235"
     CDS             13829..14245
                     /locus_tag="VLC50_07235"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HSF27294.1"
                     /translation="MRDDVEGDEPVAVGPEDPVRSIMVWPVVQVEGRTTLREVSERMR
                     AAGIGAVMVLDRGVGTGIMTERDVVRALAEGGDPDAIWAADVMGVQTLWAKPDDTIGS
                     VAELMRLAEVRHVPVREHGEIVGIVSVRDILDAFLL"
     gene            complement(14254..15351)
                     /locus_tag="VLC50_07240"
     CDS             complement(14254..15351)
                     /locus_tag="VLC50_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011691708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HSF27295.1"
                     /translation="MSERVWRSAEAKLRTTVEKHPGRFPLYTEGGAWALDGQQWTRWG
                     DGFLGGALWLVAAHGEDRVWFRQQAERYTRLVEGRKADDQIHDHGFLFWPTYYRWYQE
                     TQDPAHLAVLVEAARTLAGRFREPGRYLPSFLGPESLFIDIMMNVHLVLFAASVTGDR
                     ELARIGHEHSLTTRRFLIRGDGSASHAGVFDVQTGAFIRQTSQQGFADDGSWARGQAW
                     AIHGFGTVYRFTGDRRYLATAVSAADFYLESTGDRLVPPNDWTEPEPARPFESSAAAA
                     AAAGLWQLAGLVPDAGLASRYADYAARILVRLSQDDFLASPDEPWEGVLKHGSYHEGL
                     GLGVDESLIFGDYWFLAAVSAVTQDFSAPRR"
     gene            complement(15429..16238)
                     /locus_tag="VLC50_07245"
     CDS             complement(15429..16238)
                     /locus_tag="VLC50_07245"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019925877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSF27296.1"
                     /translation="MLEVRHLRKVYEGSGRSVEAVHDVDFSLRKGELVCVVGPSGAGK
                     TTILRCISGLLSPTSGEVVLDGRTVDGPPDGMAVVFQDYGRSLFPWMKVRANVELPLK
                     EKKVPAEERRRLVESSLDAVGLASAANAYPWQLSGGMQQRVAIARAIAYEPAVLLMDE
                     PFASVDAQTRADLEDLVRSLWTRLGVTIVFVTHDIDESVYLGERVVVLSASPTVVMED
                     VAIDLEQERDQLTTRAQARFGQLRTHVYELIQKAKDSGHREDLLPAGESHQ"
     gene            complement(16308..17072)
                     /locus_tag="VLC50_07250"
     CDS             complement(16308..17072)
                     /locus_tag="VLC50_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005483550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HSF27297.1"
                     /translation="MAGLKKAWTIFLVPVTLFAIWLLTSSDSTSFFFPSLRKILSAFA
                     HTWFSQRWSADIVPSLARLAAGFLIALALALLLGVILGLSKRARDYVEPALEFFRAIP
                     PTVMIPVLLVISGVGNTSKVAVIAFGCIWPMLLNTIEGVRSVDSLLKDTCDIYKIHGL
                     RRLRILTLRAASPQIVTGMRTGLSIAIILMVISEMFASKDGLGFTIIQFQRTFSIPEM
                     WSGILLLGIIGVVLSLLMRVFEMIVLRWYEGARKRD"
     gene            complement(17085..17873)
                     /locus_tag="VLC50_07255"
     CDS             complement(17085..17873)
                     /locus_tag="VLC50_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017833934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSF27298.1"
                     /translation="MTKPPKKSASSARHRGIIGFLALLAVWEVLPASDVISARYFPPL
                     HEVAVAMADNLVDSGFWLAVSHTVQSWFLGLVIAFAAASLVGLILGSISILWRYTDST
                     IEFLRPIPSVALIPLAVLMFGIDMKSALLLIVYAAFWQILIQILYGVKDVDPVALDTA
                     RVFGLRRWSQIRYVSWPTALPYAITGFRLGASVALVLAVTAELVIGNPGIGHEIALAE
                     SALALPEMYSLVITAGVMGVLVNQLTRFVERRALSWHQSVRSEA"
     gene            complement(17885..18898)
                     /locus_tag="VLC50_07260"
     CDS             complement(17885..18898)
                     /locus_tag="VLC50_07260"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HSF27299.1"
                     /translation="MSRLNTVGEQMRKLAIASAVALAVSLGMTGCGSSADPTPADAAN
                     SSGPVKVETLRAGLLPNAETSAIRLGDQKGFFDDAGIKLEITDTSSGAASITALVAGQ
                     FDIVFANTVSVMQGRDKGLPLVLVAAASTSTGVAGKDFSGLMVASDNKMTSAAELAGK
                     TIASNTIKNIGELTARLSVKKAGGATDGLNFVELPFANMEQALEEHQIDAAWMVEPFH
                     TTALQHGLRDIASNYVDTAPGLTAAAFVTTEKVIKEKPELIKRFKEAITEAGNYANAH
                     PNEVRELIPTFTKVTAEIANEFVIPLFNPDVNVASLEAMLPEMVSLEMISKDFDVNSV
                     IYK"
     gene            19182..19973
                     /locus_tag="VLC50_07265"
     CDS             19182..19973
                     /locus_tag="VLC50_07265"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HSF27300.1"
                     /translation="MEASKRGSRHGQALDHAIDVLECMAEAARPIGVTEIARQVGLSK
                     ATVHHLLATLLSRRIVMQDPHSSLYRLGWALYELGSSVVADIELSRVARTHLDRLAAV
                     SGESVLLGILNGDTVLYLDRGDPRGGLHLSTTGHRGSLHATASGKVHLAFCGDPSLLD
                     RVLGGPLARLTRSTVVDPETLRGQLRVVRSRGYATSWQEREVGVCSVAVPLRDRTGRA
                     IGSLSLVGPAARLTPRTLPTQLSRLKHTASQIERGLGCSSAVAEG"
     gene            complement(19979..>20163)
                     /locus_tag="VLC50_07270"
     CDS             complement(19979..>20163)
                     /locus_tag="VLC50_07270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="acetamidase"
                     /protein_id="HSF27301.1"
                     /translation="DDAVRMAVDGMHESLCRRGLDPVEAYMLLSIAGHVGVNQVVNAP
                     HVGARITLAKAVLAPH"
BASE COUNT         2796 a         7175 c         7266 g         2926 t
ORIGIN      
        1 tatctcggct acgtcaccgg gctggccggc accgacctgg ccgcggcccg gcgcgggcgg
       61 atgctggccg gcaccagcct gttcgtcgcc gggttcaccg tggtgttcgt cgcctacggc
      121 gtgctcttcg gcgggctggg caaggcgctc atcgagtacg gcgacgtgat cacccgcgtg
      181 ctcggcgcca tcacggtggt gctcgggctg gcgttcatgg gcctggtgcc cggcctgatg
      241 cgcgagtggc gggtgcaccg ccggccgggg ctgggcctgg ccggtgcgcc gctgctgggg
      301 gtgctgttcg ggctgggctg gaccccgtgc atcggcccca cgctcggggc ggtgcagtcg
      361 ctggcctatg acacggccag cgccgggcgc ggcgcgctgc tgtcggcgat ctactgcatc
      421 gggctgggac tgccgttcct ggtcgcggcg ctggcctatc ggcgcatgct gggtgcgttc
      481 gcctgggtgc gccggcacag cctgtgggtg acccggctgg gagggctgat gctggtgctg
      541 atcggcctgc tgctcgtcac tgggctgtgg accgagctca cggtcgcgct gcgaggctgg
      601 gtcggcggat tcgagacggt gctgtgatga gcgccgaccc cacccccacc ggggcgccgg
      661 ccgctgccgg cctgtccacc gcaccggtgc cgacccccga ccggcccggt gccctgcgcc
      721 cccgggagtt cgcccggtgg atctggcggc agctgacctc gatgcgcacc gcgctggtgc
      781 tgctcttcct gctcgcggtc gcggcggtgc ccggctcgct cgtgccgcag gagtcggtca
      841 gcccgatccg ggtggtcgac ttcaagcgcg accaccccac gctcggcccg atctatgagc
      901 ggctcgggat gttcgacgtc tacacgtcgc cgtggttcgc cgcgatctac ctgctgctca
      961 tcgtctcgtt ggtggggtgc atcctcccgc gcagccgggc gcacctgcgt gccctacgcg
     1021 ccaccccgcc ggccaccccg cgcaacctgg cacggctggc ggcctatcgc acctggggca
     1081 ccgacgcgac gccggaggcc gtgctggaga gatctcgggc ggtgctgcgc aagcggcgct
     1141 tccgggtggc tcccggcgac ggctcggtgg ccgccgaggc cggctacctg cgggagaccg
     1201 gcaacctggt gttccacatc gcactgctgc tcatgctcgc cgccgtcgcg ctgggcagcc
     1261 tgttcggctg gaaaggcacc gtgctggtgc ccgagggctc ctccttcgcc aacaccgtct
     1321 cggagttcga cagcttcacc aagggcgcgc tggtcgacga gcgggcgatg gtcccgttca
     1381 cgctggccct ggacaagctc gacgtgcgct accagcagac cgggcagcag gccggcgctc
     1441 cgcgcggctt caccgccgac gtcaccttcc ggcccgaccc cgccatgccc gcgcagcgtt
     1501 cgcgcatcca ggtcaactcg ccgctggtgg tggacggcac caaggtgttc ctcatgggca
     1561 acggctacgc cccgcggttc acggtgcgcg acgccgccgg gaccgtggtg ttcgccggtg
     1621 cggtgccgtt cctgccgcag gagtccacga cgttcacctc gaccggggtg atcaaggtgc
     1681 ccgacatcaa gccggagcag ctcggcttct cggcgctctt cctgccgacc gccaaggttg
     1741 accccgagct cggcccggtc tcggtctttc ccgacgcgct caaccccgag gtcttcctgg
     1801 gcgcctacaa gggcgacctc ggcatcgacg ccgggctgcc caagtcggtc taccgcctcg
     1861 acaccgacga catgaccctg gtcgccaacg ggcgcctgct gcccgggcag accctgacgc
     1921 tgcccgacgg gctgggcagc atcacctacg acggctggac ccgctgggca aacttccagg
     1981 tcgccgacga cccgggccgg ttccccgcgc tggtcgccgc gctcgccgcg atcggcgggc
     2041 tgatgctgtc gttgttcatc cggcgtcgac gggtctgggt ccgagcggtg gccggtccgg
     2101 gcgggcgtac cgtggtcgag gttgccggcc tggcccgctc cgagtcgacc gccctcgagg
     2161 acgagatcgc ttccctcacc gacgagctgg gcggccccgg cgagtcgcgg ccagacccca
     2221 gtaccaacag caccggcaag gagtgaccgt ggcgatcgac gtcgggctcg cgacgctgag
     2281 caacaacctg atctactccg cgatggtcgt ctacaccggc gcgatgtact gctacgccgc
     2341 cgagctggcg ttcggcaacc gcagcgcgcg ccgggtcgcc gccacggagc gcgagctggc
     2401 ggccgtcggg gcgccggcgg cgggggcccc tgccgggctt cccacggccg acagctcggc
     2461 cgcttcgtcg tacggcgacg aggcgagtgc gccccagccg ctgccgctcg ggcaccggat
     2521 gggccggatc gcggtctcgc tgaccgtgct ggcgttcctg ctccacctgg ccgcggtggt
     2581 cactcgcggc gcctcggcgg ggcgaatgcc gtggggcaac atgttcgagt tcgccaccgc
     2641 ggcggcgctc gccgtgaccg gggtcttcct cgtgctgctc gcacgcaagc cggtgcgcta
     2701 tctcggcctg ttcgtcgtcg gcccggtgct gctcatgctc gggctggccg tgacggtgct
     2761 ctacaccgag tccgagcagc tggtgccggc gctgaagtcg gtctggctgg gcatccacgt
     2821 caccgcggcc atcgtcgcga tggggctgtt caccgtcggc ttctcgcaga cgatcctcta
     2881 tctcatccag gcccgccgcg agcgggtgcc ggcggccgag cgcacgccca acttcatgga
     2941 ccacttcccc gctgcggcgg tgctcgaccg ggcggcctac cggatgcatg ccttcgtgtt
     3001 cccgctgtgg acgttcgcga tcgtggccgg ggcgatctgg gcggagaacg cctggggtcg
     3061 ctactggggc tgggacccca aggaggtctg gtcgttcatc acctgggtgg cctacgccgc
     3121 ctacctgcac gctcgggcga ccgccggctg gcgcgggcac aaggcggcct acgtcgcgct
     3181 gctcgcctac ggcaccatcc tgttcaacta cctcgtggtc aacatctggc tggtcggctt
     3241 ccactcctac tccggcgtct gagcggctca gcggcggcgt agcgcgtcga cgaccgtctg
     3301 cacggccatg aagcctgccg cgagcagcag caggatcccg aagccgacgc gggcgttgcc
     3361 gaccaccagt gcgacggccg cgatcgcggc gatcaccact agggccaggg tcaccaggct
     3421 gatgatcagc gccagcagcc ggcggtgcac cactgcctcg atgacgagca tcacggcgac
     3481 caccacgagg ctgaccccca gcacgtcgga gctgctgctg agcagcacca gcccgagcgc
     3541 ggcgaacagc accgaggccg acgcggaggt ccacacccgg gccagccacg agcggttgac
     3601 cggagtcttg gagatcggca gcggccggtg ccgcaggtgg gcgtgcaccg ggtcggtggt
     3661 cgacacgcgt gagcgcccgg cccggcccgc cgcctcgtcg gcccgttcgg tggccacgac
     3721 cgtggcctcc agcgagcggc acagccggtg ctgctcctcc cgcagcgcgg ccactcgggc
     3781 ggccatcgcg cgcacgtcgg cgccgggggc ccggggcgag cgctgctggg tctggtcgag
     3841 ggcccgggcc ccgcgcagct cgtcgcgggc gtgcgccagc tcgacgtcca gctcggccac
     3901 ccgctggcgc aggtcggtgg cccgccggcc gagggccgcg gccgcctcgg cgctcgtcgg
     3961 cgcctcgctg tcgagcgccg cccaggcgac cggctggctc cacgacgcgc gcacccggcc
     4021 atcacgctcg tagcgcgggc ccgcgggtgc gcgctcgccg ccgagcgggt cgtgggtgtc
     4081 gagcccccac aggccggtgt agtcgcgcac ccacggggtg gtgtcgtcga tgacctcgac
     4141 ggtccactgc tgctcgtgcc ccggcccgac cgcgcggccg tcgccgcggt ggtagtcgat
     4201 gaacgggatg ccgatgccgt ccgggtcggg agaggaccac gggatgaccc gcatcagccg
     4261 cctccgcgtg gcctccagcc aaccgggcag cggcggactg acggtgatca ggtagtcacc
     4321 gggcaggcag gagcctgagt gagacccggc gcccgcatag accaccgggt gcaggcccac
     4381 ccactcgagg tccgggtcgt cgcacctgcg gcgcaggtcg gcgccggtct cgtcgtggct
     4441 ggagaacgcg acccaggccg gggtcgacgt gccgtcgtgc tcgaccgcga acacggtgac
     4501 ctgctcccag tcggcctcgt ggtcgttgac cccgccgaac gtcgatcgcc agtcgttcat
     4561 cgcgtagaag aaccagtact gcagcacgag gtagccgccg tcgcgggtca cgtggccgta
     4621 gtagtgggcg cgctggtcgc ccccggcagc ggcgatcgcc tgctcggccg cggccgcgta
     4681 gccgccgggt acccgcccgc gcaccagcag ggagagccgc atgagcgcgg cgaccaggcg
     4741 ggccagcagg ccgaccgcgg ccgcgccgct ctcgccgacg aaccgcaccc gctcggggcg
     4801 ccgacgccac tcccgatagg cccgccgtcc caacggcgcc tccacgaagc gcaaccacag
     4861 ccgctcgccg tggtgggccc ggccgatgct cgcgagcgcg gtcgtgtcca gcgtcgtggg
     4921 cgcggcgagc tgttcggtcg tgtcgtcggc gacgcgaaag agcgccgcac tgcgcagata
     4981 ggcctcgacc gaggacggga ggaacagctc gccgctggtg tagcgcacga ctggctcgtg
     5041 ccggcgcagg aggtcttcgg ccgaggtcgt gggcacgtcg ggggtggcgg acatccgcac
     5101 ctccgacccc gaccggcggc tggactacga ggctagcggg gcgaggcggt cacagaggcg
     5161 gtcacgggct gggtggtgag ccatcgcccg acggcggctc gccgttgtcg gggctgccgt
     5221 cctcgcgcag ccgccgctcg cgctcgcgca ggtcggcttc ccacttctcg agcagcgcct
     5281 ggtgctcgtc gtcgagcccg cgcaggctgg ccaggaactc cgggtcgtcg tcgggggcca
     5341 ccgcccgctg cgtggccctt ggcgcgcgcc ggagcgggcc ggccgaccct ccgccgagcc
     5401 agccctgggt gctgcgggtc ccggcctgcg cgtcattggc cggtcggccg gcgaacagcc
     5461 aggccagcgg gccgacgatg ggaaagagca ggatcagcag cagccagaac ggcttgggca
     5521 ggttgcgcat cgcctgctcg tcggtctgca gcgcgtcgat gaggcagtag acggtcagcc
     5581 cgatgctcag caggatcggc aggacccggg acacacccac ctccaccgtc ggggctctgc
     5641 ccccagccta gacgccggca tcgtccgccc cgaggtcgga cgaggccggg ccggtccagg
     5701 cggaggcggg tcggcctagt ggtgccagag gtcgcgcagc cggcgccggg cgcgcggccg
     5761 tcgtacggag ttgagccttt cggcctccgc ggcacgctgc aggtcgcgga ccgcgacccg
     5821 ggcgagctcg agggtgatca tctctaccat cctgttcgtc ggcttcccaa ggccatctcg
     5881 gcaagcgctg ttgcgattct atcgcatgtg cgcggtatga ccttcgaaac gattgtgata
     5941 tcgggcaccc gccagtacca tcgccaccca cagggaggtg cccatggacg ccatcgatcg
     6001 ccgcctcgtg gaggagctgc gggccaacgg ccgggccagc tacgccgagc tgagccgact
     6061 ggtcgggctc tcggcgccgg cggtgcagga ccgggtccgc cggctcgaga cgaccggggt
     6121 gatcaccggc taccacgccg ccgtcgatcc gcaggcgctc gggctcggcg tcagcgcgct
     6181 ggtcagcgtc taccagagcg actccaccga ccaggacgac gtggccgctc ggctggccga
     6241 ggtgcccgag gtcgagagct gctggttcgt cgccggcgac gaggcgttcg tgctcaccgt
     6301 gcgggcccgc gacatcgccg cgctcgagac ggtgctcggc accctgcggc gcctgaaggg
     6361 ggtggcccgc actcgtacgt cggtggtgct ctcgacccgc tgggaggggc gggtcgccac
     6421 gaccgggtga cggcaccgac ccggccgagg ctgtccgccc cggtcggcgg cagcggttgc
     6481 ccgcgccgta cgctcgtgcc gtggctgctc cgcccgcgct gcgctactcg ctcctgcgcg
     6541 tcaccctgtt cgtcgccgcc ctggcgctgt ggttcgggct ggggttgcgc ggtttctggc
     6601 tgctaatcgc cgcggtgctg atgtctggcg tggtctccta cttcctgctg accgaacccc
     6661 gggcggccat gtccgagcgg ctgtccgcgc ggttcagccg gctgggccgg cgcatcgacg
     6721 agcgcacccg ggccgaggac gacgacgagc acccggacga cggtcagagg aacgtctgac
     6781 cctcgccccg gtaggtcggc gcggcgtcca cgacccggtc gccgtcgacg aggtgcagca
     6841 gcgcgaaccg ttcggcgagc tcgccggcct tggcgtggcg cagccagacc cgatcgccca
     6901 cccgcagccg gtcggcgccc ggcccgtgca gcggggtctg cacctcgccg gccccctcgg
     6961 tcgcgctcag ccgcaggccg gcgggcaggt agggcgtggg caggcggtcg cgctggcccg
     7021 gcccggaggc caggtagcca ccgccgagca cggtgaccca gccggggccc ggacgccgca
     7081 cgacgggcag cgcgaacaac gcggccggcc gcggccggaa gcgcgagtag ccgtcgaaca
     7141 gcgccggccc gtagagcccc gacccggcgg ccagctcggt cagcgtgggg tcggcggcac
     7201 tggtctccag cgatccggtg ccgccgccgt tgacgagctc gagcggcgcg gtcgcgaccg
     7261 cccgcacggc atcgatcatc gcggcccgcc gctcgtgcag ctcggcgacg ctggccgcct
     7321 gcatgcgccg tacggcgaac ccgcgaaccg ccgccgccgg cccgccgcgc ggggcgtcgg
     7381 ggacgccggc cacctgggcc tcgtaggcca tcacgcctac cagccgaaac cccggccggc
     7441 gcaccagcgc ctgggccagc ccgcgcgcct gctcggggct gcgcaccggt gaccggcggg
     7501 cccccacgtg cacccgttgg cccagccgtc ctcggggctc gagcgggcgc caggcgacgt
     7561 cgagctcgag gcagacccgc accggcgggt gcccggcccc catcgcggcg tcgaccaggt
     7621 cgagctgggc gaggtcatcg accatgatcg ccacggcggc cagggcccgc tcgtcggcgg
     7681 ccagcgcccg cagcgcggcc cggtcggccg tggggtaggc gacgacgacg tcgtcggtca
     7741 cgcccgcggc gaccagccac aacgcctcgg gcagcgtgaa ggccagcacc ccgtggaacc
     7801 cgggccgggc cagcgcccgg atgagcaggg cgcgggagcg gaccgacttg ctggccaccc
     7861 ggaccgggcg cccgccggcc cgggccacca gggcgtcggc gttggcgtcg aaggcggtca
     7921 ggtcgaccac cgccagcggg gggtcgagct cggcggtcgc gtggtcgagc cgggcccgca
     7981 gcgtctggag cgcctcggtg tcggcgcgcg gggatccgat caagccagcc accgcgccag
     8041 tatggtcggg cccgtgagcc cccgctggac caactggtcg ggcacggtgc gcacgcagat
     8101 ggtccggcag gagcacccgc gcgacgtcga ggcggtcgcg gccgccgtac gcgcggcggg
     8161 ccgggacggg ttgcgggtca agccggtcgg ggccggccac tcgttcaccg gggcggccgc
     8221 caccgacggc gtgcagctgg tgctcgacgg gctcaccggc atcgtgcacg ccgatctcga
     8281 cagcgggctg gtcacggtgc gggccggcac ccggctgcac cagctcggcc gtgcgctcga
     8341 tgcgctcggg ctggccatga ccaacctcgg cgacatcgac gcgcagacca tcgccggggc
     8401 catctccacc ggcacccacg gcacgggcgg cgcgctgggg agcctgtcct cgcaggtgcg
     8461 cgcggtcgag ctggtcaccg ccgacggtgc cgtcctcacc gcctcgacgg agtcggcgcc
     8521 cgaggtcttc gaggcggccc ggctcggtct cggcgcgctc ggggtgctca cctcagtcac
     8581 gctggcctgc gagccggcct tcctgctgcg cgccgaggag gctccgatgc cgctcgacgc
     8641 ggtgctggac gagctcgacg ggctggtgca gggcaacgac cacgtggagt tcttctggtg
     8701 gccgcacacc aggtcgacca ccgtcaagcg gctcaaccgg ctgcccgctg acgccgcccc
     8761 ggccccgcgg cacccgtggc gcgcctggtt cgacgaccgg ttcatggcca acacggtctt
     8821 cggcgcggcc accgcgctgg gccgtcgctg cccggccgcc gtaccgctgg tcaaccgggc
     8881 cgccaccacg ctggcgggtt cccgctccta cgccgacgcg tcctaccggg tgctggcgac
     8941 cccgcggctg gtccggttcg ccgagtccga gtgggcgctg ccgcgcgagg cgctgcccga
     9001 ggtgctgggc cagctcgagg cggtgacccg gcggctcgag cacccggtgg cgttcccggt
     9061 cgaggtgcgg ttcggtccgg ccgagaacgt ctggctgtct ccggcccacg ggcgggcgac
     9121 cgtctacgtc gcggtgcacc aggcgctcgg catgccgcgg gagcgctggt tcgccgacgt
     9181 cgcggcgctg gcgctggccg ccggtggccg tccgcactgg ggcaagctgc accccctcga
     9241 cgcggccagg ctgtgcgcgc tctacccccg ctttgacgac ttcgtcgccg tccgcgaccg
     9301 gctcgacccg cggggcatgt ttgccaacgc ctacctcgac cgcgtcctcg gccccgcgtc
     9361 ggggtcctag ccgagagcgt cctcgagctg cggacgtacg acggcccggc cgatcgcgcc
     9421 gtccgtcatg gcggcgaacg cgtcgttgat ctggtcgagc cggcactgct ggccgaccag
     9481 ggcgtcgagg ggcagccggc cgtcgcggta gagccgcagc agccggggga tctcgaagag
     9541 catgttggag gagccgtaga ggctgccgac gatgcgcttc tcctgctgga ccagctcgtt
     9601 gaccggcacc tggaaggtcg ccccggcctt ggtcaggccg acgacgacga tggtggcccc
     9661 gaccccggcg atgccgacgg cctgctccag ggtcgccggc gcgccgaccg cgttgagcac
     9721 ccaacgcggg gccggcgacg cgatcgcggc gatctctgcc cggacctcct gggtcgccgc
     9781 gttgaccacg tgcgtggcgc cgagctcgag agcgcggcgc agcctggtct ccacggtgtc
     9841 gaccacgatg atcgggttgg cgccgatgac ccgcaaaccc atgaccgcac tcaggccgac
     9901 cccgccggcc ccgatcacca cggccgcctc accggtggca ccgcgcatct ggttgagcgc
     9961 cgcacccacc ccggtgatga cggcacatcc catgatcgcg gcgatctggg gcgggacgtc
    10021 gccgctgatc gggatgaccg agcggtcgga gacgatgcac cgctgcgcga agcaggagac
    10081 ccccatcagg tggtggatcg gctccccgcg cagccgcagc ctcgaacggc cgtcccagag
    10141 cccgccggag acggcctggg ccttgcccag cgcgcacagg ccgggccgcc cggtggagca
    10201 ctgctcgcag tcgccgcacc ggggccgcca cgtggtgatc acggtgtcgc ccggcttgac
    10261 gatggtgacg cccgggccga cctgctccac caccccggtg ccctcgtgac cgagcaccgc
    10321 gggcagcggg gcgaccaggt ccccgttgat gtagtgcagg tcgctgtggc agacgccggc
    10381 cgcctctacc cgcacgagca cgtcccccgg cccgggttcg gtcagctcga cgtcttcgat
    10441 gacgagcggg tcgcccgggc gatggagaac ggcggctttc atggcgggct cccgggtggc
    10501 tcggggataa agtgcgaggt ccggaatatc agacaaggaa tgctgacgtc gatgcagagt
    10561 ggcgcgagcg gcccgaccgc cgcggtgggc gaggtcgtcg aggccgccag cgaggcattc
    10621 gactcgtggg gcacgtcgac gctcgatgag cgggcgaccg cgttgcgggc cgtggctgcg
    10681 gccctcgatg cgagcgccga cgagctggtg ccgttggccg gggccgagac gcacctgggc
    10741 ctcgaccggc tgcgcggtga gctgcgccgt acgacgttcc agctgcggtt cttcgccgac
    10801 gaggtgcgca ccggccgtca cctcgaggcc accatcgacc acgccgacga gcactggggc
    10861 atgggcccgc gccccgacct gcggtgcgtg gcggcgccgc tggggccggt gctggtgttt
    10921 gcggccagca acttcccgtt cgcattcagc gtcgccggcg gcgacaccgc ctcggcgctc
    10981 gccgccgggt gcccggtggt cgtgaaggcc cacgagggcc acctcgagct ctcgcgcgcc
    11041 accgcgaggg tggtgcgcga cgcgctggcc ggggtcggcg ccccgcccgg gctgttcgac
    11101 ctcgtcgtcg gcgtcgaggc cgggcgcgac gcgctggtgg acccgcgggt caaggccgcc
    11161 gcattcaccg gctcgatcgc cggtgggagg gcgctgttcg acctggcgat ggcccggccc
    11221 gagccgatcc cgttcttcgg cgagatgggc agcgtcaacc ccacgttcgt cacccgcgcc
    11281 gccgcggccg cgcgagccga gcagatcgtc gcgggcttcc tcgactcgtt caccaagggc
    11341 gccggccagt tctgcaccaa gcccggcatc ctgctggtgc ccaccggcag ccagctgacc
    11401 gagcgcctcg cggcagcgtt gtccgcacga ctgtccgtgc ctgccgcgcc gttgctcaac
    11461 caacgcatcc acgacggctt cgtgcgcggg ctggccgcgt tgcaggcccg cgagggcatc
    11521 cgggcgctgg tgatcggtga cgacgcgctc gccgatccgc cgaccccgac gctgctgctc
    11581 acgacgatcg agcggatcct ggctgccccc gacgaggtca tcaccgaggt cttcggaccg
    11641 accgcgctgg tcgtggagta cgagcgagag tcgcagctgc tcgacctggc ccgcctgctg
    11701 ccggggcagc tgagcgccag catccacggc gtggacgacg accccatcgc cccccagctg
    11761 atccagcttc tggcgcagcg ggccggccgg gtgctgtggg gccagtggcc gaccggcgtg
    11821 tcggtgacct acgcgcagca gcacggcggc ccctaccccg ccaccacggc cgcctccacc
    11881 acgtcggtgg gagcagccgc gatccgacgg ttcctgcgcc cggtcgccta ccagggcgtg
    11941 cccgacctcc tgctcccgcc gtcgttgcag gaggccaacc ccctcggcgt gccgcggcgg
    12001 gtcgacggca acagcgccgg ctaagccgag ctgctcggct cccaccacag ggtgacgatg
    12061 tcctggtggg ccgggtcgcc ggccgtgaag ccggcctcgc gcatccaggc cgccacctcg
    12121 gcctcccgct cgatcggcag gcacagccgg cgccgcatcg tggccacccg ctcgtcccag
    12181 ggcgcggcgc gggtccgccg tcgcccggtc tcgacctgta cgtcgtagcc ggcttcgtgc
    12241 acgagcgcgg ctcccagttc ggcggtcggc ccgctcggac ggggctggcc gtgcgcgcgc
    12301 gcccagagcg ggcccagccc cacccacgga tgcgccgcgg tcagctccac caccacccgc
    12361 cggcgggcgt gctccgtcag cgcccgcacg aacggcaccg cgtcggccac gttgtagagc
    12421 acgtggtggc acaccacgac gtcagccgtc ccaacctcac cggccaccgc cggccagctg
    12481 ccctcgaccg tctcggcggc cacttcgtgc tcgagggccg cccgcgcgaa cgctgccagc
    12541 atgtcgggca actcgtcgac ccccaccagc cgctgcgccg gcggcgccag cgccagcgag
    12601 gcggccccgg caccgcagcc cacgtcgagc acggcgccac cggcgggcag cgcctcgagc
    12661 gcacgatcgc gcgaggactg cggcccgggg tcgaaggcac cgcggtgctc ggcctggaag
    12721 tgcgccggcg gcagccggta gggcggctcc ggggccgcgg ccaggatcgc cgggtcgatg
    12781 gcccaggccg ccagctcggc acgccagcgc tgtgccgcgg tcggctcacc catggcgcca
    12841 gtctgcccgc cgcgagcttc gcctcagggc agggcgaggg ccagggcgaa cagcgcggca
    12901 taggccagct cggcccgccc ggtgtcgcgc agcaccggga tcagcgccgg cccgaccacg
    12961 ccggcgcgca cggtcgccac cgcgcgggcc accacggcga gggcgagcag ggtcagcacc
    13021 gtccaggggt ggcgcaccgc cacagccagc gcgaccagct gggcgagcac cacgagcccg
    13081 acatagagcc ggcgcgaggc ggcgtcgccg agcacgacgg ccagcgtccg cttgccggcc
    13141 gcggcgtcgg aggggatgtc gcgcaggttg ttggccacga ggatcgcgca ggcataggcg
    13201 cccaccccga ccgcggccgc cagcgccggc caggtgatgg tgccggcctg cacataggtc
    13261 gtgcccagca ccgcgaccag cccgaagaag acgaacaccc ccacctcgcc gagcgcgtgg
    13321 tagccgtagg ggtgcgctcc gccggtgtag gtccaggctg ccgcgatcgc ggccaccccg
    13381 acgagcagca gccaccacgc cgaggtggcc gccagcacga acccggccac ggccgcgagc
    13441 gcgaacgagc cgagcgcggc cgccttgacc gccccgggcg aggccgttcc cgacccgacg
    13501 agcctgagcg gacccacccg tacggcgtcg gtgccgcgca cgccgtcgga gtagtcgttg
    13561 gcgtagttga cgccgacctg cagggcgagc gccaccatca gggccagcaa ggccttccac
    13621 cacaccgccc cgtcgacggc caccgcggcg ccggtcccgg cgagcacggg ggacaccgcc
    13681 gccggcaggg tccggggcct ggctccttcg atccactcgc tcgtgcgcgc cacggcgtcg
    13741 tacggtatcc gggccctcga ccggctcgat catcccgggg gacctaggcc cctaggcgcg
    13801 cctcgcgggt gggcccgagc ctggtcgcat gcgcgatgac gtggagggag acgagccggt
    13861 cgcggtcggc cccgaggacc cggtgcggtc gatcatggtc tggccggtgg tgcaggtcga
    13921 gggacgtacg acgctgcgcg aggtgtccga acgcatgcgc gcggccggca tcggcgccgt
    13981 gatggtgctc gaccgcggcg tcggcaccgg gatcatgacc gagcgcgacg tcgtgcgggc
    14041 gctcgccgaa gggggagacc ccgacgccat ctgggccgcc gacgtgatgg gcgtgcagac
    14101 gctgtgggcc aagcccgacg acaccatcgg ctcggtcgcc gagctcatgc gactggccga
    14161 ggtccgccac gtccccgtcc gcgagcacgg cgagatcgtc ggcatcgtct cggtgcgcga
    14221 catcctcgac gcattcctgc tctgactccg aggtcatctc ctgggggcgg agaagtcctg
    14281 cgtgacggcg gaaacggcgg cgaggaacca gtagtcgcca aagatcagcg actcgtcgac
    14341 gccaagaccg aggccctcgt ggtagctgcc gtgcttgagg actccctccc agggctcgtc
    14401 gggggaggcg aggaagtcgt cctgcgagag gcgcacgagg atccgcgccg cgtagtcggc
    14461 gtagcggctc gccagcccgg cgtcgggcac cagacccgcc agctgccaca gtcccgcggc
    14521 tgccgccgcg gcggccgagc tctcgaacgg gcgagccggc tccggctcgg tccagtcgtt
    14581 gggcggaacc agccggtcgc cggtgctctc gaggtaaaag tcggcggcgc tgaccgcggt
    14641 ggccaggtag cgccggtcgc cggtgaaccg gtagacggtc ccgaacccgt ggatcgccca
    14701 ggcctgcccg cgcgcccagc tgccatcgtc ggcgaagccc tgctggctgg tctggcggat
    14761 gaacgcgccg gtctgcacat cgaagacgcc ggcgtgggag gccgaaccgt cgccccgaat
    14821 gagaaaccga cgggtcgtga gcgagtgctc gtggccgatc cgggccagct cgcggtcccc
    14881 ggtcaccgag gccgcgaaga gcaccaggtg gacgttcatc atgatgtcga tgaacaggct
    14941 ctccggcccg agaaagctcg gcaggtagcg cccaggctcg cggaagcggc cggccagggt
    15001 tcgtgccgcc tcgacgagga cggccagatg tgcagggtcc tgggtctcct ggtaccagcg
    15061 gtaataggtc ggccagaaca ggaagccgtg gtcgtggatc tggtcgtcgg cctttcgccc
    15121 ctcgaccaga cgggtgtagc gctcggcctg ctggcggaac cagactcgat cctcaccatg
    15181 cgcggcgacc agccacagcg cgccgccgag gaaaccgtct ccccaccggg tccactgctg
    15241 gccatcgagg gcccaggcgc cgccctcggt gtagagcggg aagcggccgg ggtgcttctc
    15301 caccgtcgta cgcagcttgg cttcggcgct cctccagacg cgctcgctca tagcggcgaa
    15361 ggaggggggc agccggaagg tgtgaggcgg tacgacgggg agcggcgggt caaggaccgg
    15421 gtagtcgctc actgatgcga ctcgcccgcg gggagcaggt cctctcggtg gccgctgtcc
    15481 tttgccttct ggatgagctc gtagacgtgc gtgcgcagct gcccgaagcg cgcctgcgcc
    15541 cgggtggtca gctggtcgcg ttcctgctca aggtcgatcg cgacgtcttc catgacgacg
    15601 gtgggtgagg cggagagcac gaccacgcgc tcgccgaggt agaccgactc gtcgatgtcg
    15661 tgcgtgacga agacgatggt cacgcctagg cgcgtccaca gagaccggac gaggtcctcg
    15721 aggtcggctc gggtctgtgc gtcgaccgag gcgaacggct cgtccatgag cagcaccgcg
    15781 ggctcgtagg cgatggcccg ggcgatcgcg acccgctgct gcatgccacc cgagagttgc
    15841 caggggtaag cgttggcggc cgaagcgagg cccactgcat cgagactgct ctcgacaagc
    15901 cgtcgtcgct cctcggcggg gactttcttc tccttgaggg ggagctcgac gttggcacga
    15961 accttcatcc aggggaacag gctcctgccg tagtcctgga agacgaccgc catgccatcg
    16021 ggtgggccat cgactgtgcg gccgtcgagc acgacctccc cgctcgtcgg cgagagcaga
    16081 ccggagatgc agcgcaagat cgtggtcttg ccggccccag aagggccgac cacgcacacg
    16141 agctcaccct tgcgcaggct gaagtcgacg tcgtggacgg cttcgaccga gcgcccgctg
    16201 ccctcgtaga ccttgcgcag gtgccggacc tcgagcatcg acccgcgggg gtctgctgcc
    16261 gcgctgggct gtgtcatcta tctaagtccc tttcaggagc tggcggccta gtcacgcttg
    16321 cgcgccccct cgtaccagcg gagcacgatc atctcgaaca cccgcatcag cagggacagc
    16381 acgacgccga tgatgccgag gagcaggatg ccgctccaca tctccgggat cgagaatgtg
    16441 cgctggaact ggatgattgt gaatcccaac ccgtccttgg acgcgaacat ctcgctgatc
    16501 accatcaaga tgatcgcgat cgacagcccg gtgcgcatgc cagtcacgat ctgcgggctg
    16561 gccgcccgca atgtcaggat ccgcaggcgc cgcaggccgt ggatcttgta gatgtcgcag
    16621 gtgtccttga gaagtgagtc gaccgagcgc acgccttcga tggtgttgag cagcatcggc
    16681 cagatgcagc cgaatgcaat gaccgcaacc tttgacgtgt tgcccacccc tgagatcacc
    16741 aggagcactg ggatcatgac tgtgggtggt atagcgcgga agaactcgag cgctggctcg
    16801 acgtagtcac gcgcccgctt agagaggccc aggatgactc ccagcagcag cgctagcgcc
    16861 aatgcgatga ggaagcctgc ggcgagccga gccaagctgg ggacgatgtc ggcggaccag
    16921 cgttgggaga accatgtgtg cgcaaaggca ctgagaatct tgcgcaggga aggaaagaag
    16981 aagctcgtgc tgtcgctcga ggtaaggagc cagatggcga agagcgtcac cgggacgagg
    17041 aagatcgtcc acgccttctt gagtcctgcc atgacggtgg acacctatgc ctcgcttcgc
    17101 acggactggt gccagctcag ggctctgcgc tcgacgaacc tggtgagctg gttcacgaga
    17161 acgcccatga ctcctgcggt gatcaccagg gagtacatct cgggcaacgc cagtgctgac
    17221 tctgccaagg cgatctcatg gccgatgcct gggttgccaa tgacgagctc ggcggttacc
    17281 gcgagaacga gtgccaccga tgcaccgagc cggaagccgg tgatcgcata aggcagtgcg
    17341 gtcggccatg agacgtatcg gatctggctc cagcggcgca acccgaagac gcgcgcggtg
    17401 tcgagggcga cagggtcgac gtctttgacg ccgtagagga tctggatgag gatctgccag
    17461 aacgccgcat aaacgatcaa gaggagcgcc gacttcatgt cgatcccgaa catgagtacg
    17521 gcgaggggga tcagcgccac cgacggaatc ggccgcaaga actcgatcgt ggagtcggtg
    17581 tagcgccaaa ggatcgagat cgagccgagg atcagtccga ccagagacgc tgccgcgaag
    17641 gcgatgacca gcccaagaaa ccatgactga acggtgtgcg aaactgccag ccagaaccca
    17701 ctgtcgacga gattgtccgc catggcgacg gcaacttcgt ggagcggcgg aaagtaccgc
    17761 gccgagatga catcgctcgc gggcaggacc tcccacaccg ccagcagcgc caggaaccca
    17821 atgattcctc gatgcctggc gctgctggcg gacttctttg gcggcttcgt cacgtctcta
    17881 ccgtctactt gtagatcacc gagttgacgt caaagtcctt ggagatcatt tccagggaca
    17941 ccatctctgg aagcatggcc tcaagggagg cgacgttgac gtcggggttg aagagtggga
    18001 tgacgaactc gttggcgatc tcggcggtga ccttggtaaa ggttggaatc agctcgcgga
    18061 cctcgttggg gtgggcattc gcgtagttgc ccgcctcggt gatggcctcc ttgaagcgct
    18121 tgatgagctc cggcttctcc ttgatgacct tttcggtcgt gacgaacgcc gcggccgtca
    18181 gcccgggggc cgtgtccaca tagttggacg cgatgtccct cagcccgtgc tgcagggcag
    18241 tggtgtggaa tggttccacc atccaggccg cgtcgatctg gtgctcctcg agggcttgct
    18301 ccatgttggc gaagggcagc tcgacgaagt tgaggccgtc ggtcgcgccg ccggccttct
    18361 tgacggacag tcgtgcagtg agctcaccga tgttcttgat cgtgttgctg gcgatggtct
    18421 tgccagcgag ctcggcggcc gaggtcatct tgttgtcgga cgcgaccatg aggccggaga
    18481 agtccttgcc cgcgacacct gtggacgtcg aggctgcggc gacgagcacc aggggcagac
    18541 ccttgtcgcg gccctgcatt acggaaaccg tgttggcgaa cacgatgtcg aactgcccgg
    18601 cgacgagagc ggtgatagag gcggcaccac tgctcgtgtc tgttatctcg agcttgatgc
    18661 ccgcgtcgtc gaagaatccc ttctggtcac caagccttat cgcggaagtt tcggcgttgg
    18721 gcaacagtcc tgcccgaagc gtttccacct tgacggggcc ggagctgttt gcggcgtcgg
    18781 ccggcgttgg atctgcgctt gacccgcagc ccgtcatgcc taggctcacg gccagcgcga
    18841 cggcgctggc gatggctagc tttctcattt gttctcctac ggtgttgaga cggctcacgg
    18901 gcgggccgac tgttcgtaat gacgcacgag acctgcgaac acgactcgca cccggggtcc
    18961 tccgagtgcg ttgcgcccag ccgttctgtg gccgtgcgcc tgaccccctg cggaccgccc
    19021 gggtccgccg ggccgccaca agtcgaatgt ataggccagg tggtgcaagg tgtgaacatc
    19081 gtttggatga gtcaaacagt tttcgatgac ggtcgtagac cgcctggatt ggtgagccta
    19141 gagtcgctga cgggtcgcat ttgatgtcag gtcaggagtc aatggaagcc tcgaagcggg
    19201 gatcccgaca tggccaggcg ctcgaccatg caatcgacgt ccttgagtgc atggcggagg
    19261 ctgctcggcc gatcggggtg actgagatcg cccgccaggt cgggctgagc aaggccacgg
    19321 tgcaccatct cctggcgacg ttgctcagcc gtcgaatcgt gatgcaggat ccgcattcgt
    19381 cgctctaccg cctcggctgg gcgctctatg agctcgggtc gagcgtcgtt gccgacatcg
    19441 agctgtctcg ggttgcgcga acccacctgg accggctcgc ggctgtgagt ggtgagtcgg
    19501 tgctcctggg catcttgaac ggcgacactg tcctctacct tgaccggggc gacccgcgag
    19561 ggggcctgca cctgtcgacg accgggcatc gaggttcact gcacgcgacg gcgtctggga
    19621 aggttcatct ggcgttctgc ggtgacccaa gcctgctcga ccgggtgttg ggtggcccgc
    19681 tggctcggct gacgcggtcg accgtggtgg atccggagac gttgcgaggg cagctccgtg
    19741 tggtccgctc acgcgggtac gcgaccagct ggcaggagcg ggaggtcggc gtctgctccg
    19801 tggctgttcc gctccgtgac cgcaccggcc gcgcgatcgg ctccctctcg ctggtggggc
    19861 cagcggctcg gctgactcca cgcacgctgc ccactcagct gtcgcgcctg aagcacacgg
    19921 cgtcgcagat cgagcggggc ttgggctgct catccgcggt agccgagggg tgagccggtc
    19981 aatgcggggc caggaccgcc ttggcgagcg tgatgcgggc tccgacgtgc ggggcgttga
    20041 cgacctggtt gacgccgacg tggccggcga tgctcagcag catgtaggcc tcgaccgggt
    20101 ccaggccgcg ccggcacagc gactcgtgca tgccgtcgac ggccatccgc accgcgtcgt
    20161 cga
//
LOCUS       DATIBB010000181         7421 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_1430827, whole genome shotgun sequence.
ACCESSION   DATIBB010000181 DATIBB010000000
VERSION     DATIBB010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 7421)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7421)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7421
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1430827"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1318)
                     /gene="pepN"
                     /locus_tag="VLC50_07275"
     CDS             complement(<1..1318)
                     /gene="pepN"
                     /locus_tag="VLC50_07275"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020074926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HSF27302.1"
                     /translation="MPGMNLTRDEARERARLVEVSSYDVALDLTTGDTTFASRTVMRF
                     RCLEPGASTFADLVAPAVHSVVLNGRTLDAAAVADGIRIQLDDLAADNVLEVVADCAY
                     MNTGEGLHRFVDPVDDEVYLYTQFEVSDARRVFTVFEQPDLKATFQFTVTAPSHWQVV
                     SNAPTPEPTEVREGVRRWAFPPTQPISTYITALVAGPYHAVRRELTSRDGRTIPLGVF
                     CRRSLAEHLDADAIVEVTRQGFAFYEDLFDYPYPFDKYDQLFVPEYNAGAMENAGAVT
                     LLEDYVFRSRVPDAMSERRAETILHELAHMWFGDLVTMRWWDDLWLNESFATYASVLC
                     QSEVTPWRHAWTTFANAEKSWAYRQDQLPSTHPISADIRDLEDVEVNFDGITYAKGAS
                     VLKQLVAWVGRDEFFEGIRRYFRTHEWGNTSLADLLAKLEQTSGRDL"
     gene            1497..2105
                     /locus_tag="VLC50_07280"
     CDS             1497..2105
                     /locus_tag="VLC50_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019157772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HSF27303.1"
                     /translation="MSEQASVGFWFDPICPWAWMTSRWMMEVEKVRPVEVTWHVMSLS
                     YLNDGRDLPDDYAELMRRAWGPVRVITAAAEEHGSGVIKPMYDAIGTRVHPGGRQDLD
                     AVLAEALAEVGLPAGLAEAAGSTDYDEALKKSHHAGMDQVGMDVGTPVIAVGGVAFFG
                     PVVTPAPKGEDAGRLWDGVVLVASTPGFYEIKRTRTQRPVFD"
     gene            2176..2373
                     /locus_tag="VLC50_07285"
     CDS             2176..2373
                     /locus_tag="VLC50_07285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF27304.1"
                     /translation="MAVVPASHYWDFREARWVVCRVPEVAAPDPASVARSEPAAEPLD
                     AAGRPTIPAQGGDRRLPSRHG"
     gene            2449..2925
                     /locus_tag="VLC50_07290"
     CDS             2449..2925
                     /locus_tag="VLC50_07290"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017573323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase"
                     /protein_id="HSF27305.1"
                     /translation="MRVHIGCDHAGFELKEHLVGWLEDQGHEVVDHGAPAYDGQDDYP
                     PYCLRAAEAVVADRGSLGIVLGGSGNGEAIAANKVRGVRAALAWNEEIARLGREHNDA
                     NVVSLGARMHTVDEATRIVAAFLDTVFSADERHARRIAMLTHYEDTGEAPAAPGVA"
     gene            2941..3792
                     /locus_tag="VLC50_07295"
     CDS             2941..3792
                     /locus_tag="VLC50_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018538887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HSF27306.1"
                     /translation="MPEGHTIHRLARELGTRLAGGPVRACSPQGRFAAGAASIDGHPL
                     ARAEAHGKHLLARFDHQRSRLWVHVHLGIYGRLTFGQAPAPAPIGLVRLRLEGPRAWA
                     DLRGANTCELLDAAGRDALIARLGPDPLRRDADPDRAWVRMQRSRAPVATLLMDQSVL
                     AGVGNIYRAEILFRHGVDPMLPGRALAREVWDAMWADLQVLMRAGVRTGRIDTVRPEH
                     DPRVTGRAPRRDRHGGEVYVYRRAGQHCHVCATPVASAVLAGRNLFWCPRCQPPGATG
                     AGLAARV"
     gene            3901..5079
                     /locus_tag="VLC50_07300"
     CDS             3901..5079
                     /locus_tag="VLC50_07300"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005317568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HSF27307.1"
                     /translation="MAAAGQPMVAGAASDGSPGRLAAVVPARSPRSSPARRVRRLWRR
                     ISHGRRLRALRSDGALLVALTLASVGLCWAGTAWPRLFPESTLALVVLAGGLMLRRRS
                     LLLLHVVVAATLAYAVVGRPSVGIGSVGVVVVTGAAVLVLSRTRTRLGVPGTSGETML
                     VDLRDRLRQQGEMPPLPAGWHAEIVLRPAGGDSFSGDFLVASRPGDGATLEVGLVDVS
                     GKGRSAGTRALLLSGAFGGLLGSMAPGGFLQAANSYLLRQDWDEGFATAVHVVVDLAS
                     GEYVVRSAGHPPIAQFAAGSGRWRLRDAARGPALGVIPGATYLGETGRLGRGDALLLY
                     TDGLVEVPGRDLSLGIDRLLGHAERLVTRGFRHGARTLIDSVASGRSDDRALVLIWRT
                     "
     gene            5107..5529
                     /locus_tag="VLC50_07305"
     CDS             5107..5529
                     /locus_tag="VLC50_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020420363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HSF27308.1"
                     /translation="MRPVHFEIHASDPTAVREFYQSVLGWRFQQWGDNPYWIVITGDG
                     DPMAGVPHSQPGVDGGLLPRLGGRPEEGQPVNAFVMTVEVPDCDAYVDKAVAAGGSVA
                     LPANDMAGVGRLAYVKDPDGNLFGMLEPAPVEEAPNGS"
     gene            complement(5524..5967)
                     /locus_tag="VLC50_07310"
     CDS             complement(5524..5967)
                     /locus_tag="VLC50_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018152993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase/bleomycin resistance/extradiol
                     dioxygenase family protein"
                     /protein_id="HSF27309.1"
                     /translation="MSTSIFVNLPVRDLDRSVAFFAKLGYGFDPQFTNKDAACLVVDE
                     SINVMLLREDFFATFTPKKVADSTGSTETIIALSAESKQRVDELVNAALGAGAVRYKE
                     PVQEGPMYGWGFQDLDGHLWELIYMEPRPGEADSSTSGGDDPGRS"
     gene            6038..6277
                     /locus_tag="VLC50_07315"
     CDS             6038..6277
                     /locus_tag="VLC50_07315"
                     /inference="COORDINATES: protein motif:HMM:NF039414.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6343 family protein"
                     /protein_id="HSF27310.1"
                     /translation="MSEPVKARSALRLRAVMSLIALPVALIAGVILLAVAPGSGEDRA
                     PYVVAAIICFAVVVIAAVDLVVIARRVKQEPRRRG"
     gene            6317..7078
                     /locus_tag="VLC50_07320"
     CDS             6317..7078
                     /locus_tag="VLC50_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015936288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HSF27311.1"
                     /translation="MRLPFVVTGDPSGPPLLLVHPWGEAHGCFDRLAPLLPQSWHVIA
                     MDQKGHGRASKPAAGYALVDLASDLAAFLDALEITRAVVLGSSSGGYVAQQLAVSHPE
                     RVAGLVLVGTPRSLRGRPAFAAEVEGLTDPVDPEWVRQSLSWFPTMAPVPSWFVAERV
                     ADGLRLPARVWRQALAGLCEAVPPTEVATITAPTLVIWGARDGLLPRADQEALVAAIP
                     DSRLIVYADTGHLVLWEQPGRVAADTAAFVKGCPG"
     gene            complement(7118..7421)
                     /locus_tag="VLC50_07325"
                     /pseudo
     CDS             complement(7118..7421)
                     /locus_tag="VLC50_07325"
                     /inference="COORDINATES: protein motif:HMM:NF025736.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
BASE COUNT          960 a         2552 c         2771 g         1138 t
ORIGIN      
        1 agaggtcgcg cccggaggtc tgctccagct tggccagcag gtcggccagg ctggtgttgc
       61 cccactcgtg ggtccggaag tagcggcgga tcccctcgaa gaactcgtcg cgcccgaccc
      121 aggccaccag ttgcttgagc accgaggcac ccttggcgta ggtgatgccg tcgaagttga
      181 cctcgacgtc ttccaggtcg cggatgtcgg ccgagatcgg gtgggtcgag ggcagctggt
      241 cctgccggta ggcccaggac ttctcggcgt tggcgaacgt cgtccaggcg tggcgccacg
      301 gggtgacctc ggactggcac agcacgctgg cgtaggtggc gaacgactcg ttgagccaca
      361 ggtcgtccca ccaccgcatg gtgaccaggt caccgaacca catgtgcgcc agctcgtgca
      421 ggatcgtctc ggcgcgccgc tcggacatcg cgtcgggcac ccggctgcgg aacacgtagt
      481 cctcgagcag cgtcaccgcg ccggcgttct ccatcgcccc ggcgttgtac tccggcacga
      541 acagctggtc gtacttgtcg aacgggtagg ggtagtcgaa caggtcctcg tagaacgcga
      601 acccctgccg ggtcacctcg acgatcgcgt ccgcgtcgag gtgctcggcc agcgagcggc
      661 ggcagaacac cccgagcggg atggtccgcc cgtcacggct ggtcagctca cgccgtacgg
      721 cgtggtaggg cccggccacc agcgcggtga tgtaggtcga gatcggttgg gtgggcggga
      781 aggcccagcg ccgtacgccc tcgcgcacct cggtcggctc gggggtgggg gcgttggaca
      841 ccacctgcca gtggctcggt gcggtgaccg tgaactggaa cgtcgccttg aggtcgggct
      901 gctcgaagac ggtgaacacc cgccgcgcgt cgctgacctc gaactgcgtg tagaggtaga
      961 cctcgtcatc gaccgggtcg acgaaccggt gcagcccctc gccggtgttc atgtaggcgc
     1021 agtcggcgac cacctcgagc acgttgtcgg cggccaggtc gtcgagctgg atgcgaatgc
     1081 cgtcggcgac ggccgcggcg tcgagggtgc gaccgttgag caccaccgag tgcaccgccg
     1141 gcgccacgag gtcggcgaac gtcgaggccc ccggctcgag gcagcggaac cgcatcaccg
     1201 tgcggctggc gaacgtcgtg tcgccggtcg tgaggtcgag cgcgacgtcg taggaggaga
     1261 cctcgacgag tcgggcgcgc tcgcgggcct catcgcgggt gaggttcatt ccaggcacgg
     1321 cgcagtgctc cttggcagtc ggatcgtccg gtaccggggg tccggtccga cggaatcctt
     1381 gcacgagccg gccggatgcc tgaggagttg tggcggccgg ccgggcggaa tgggggccgg
     1441 tgcgggcggg ttggaccagg atgcatcggg gcggacgcga aggagaggat ggccgggtga
     1501 gcgagcaagc gtcggtgggg ttctggttcg acccgatctg cccgtgggcg tggatgacct
     1561 cccgctggat gatggaggtc gagaaggtcc gcccggtcga ggtcacctgg cacgtgatgt
     1621 cgctgtccta cctcaacgac gggcgcgacc tgcccgacga ctacgccgag ctgatgcgcc
     1681 gcgcctgggg gccggtgcgg gtgatcaccg cggcggccga ggagcacggc tcgggcgtga
     1741 tcaagccgat gtacgacgcg atcggcaccc gcgtgcatcc gggcgggcgc caggacctgg
     1801 acgcggtcct cgccgaggcg ctggccgagg tcggcctgcc ggccgggttg gccgaggcgg
     1861 ccggaagcac cgactacgac gaggcgttga agaagagcca ccacgcgggc atggaccagg
     1921 tcggcatgga cgtcggcacc ccggtgatcg cggtgggcgg ggtggcgttc ttcggacccg
     1981 tggtcacccc ggcacccaag ggtgaggacg ccggtcggct ctgggacggc gtggtgctcg
     2041 tcgcgtcgac gccgggcttc tacgagatca agcgcacccg cacccagcgg ccggtcttcg
     2101 actgagcccc cttccgatca cggcgcggca ggagcatcgt cgtaccgatc gcccctgcgt
     2161 cgagtcggag aggccatggc cgtcgtcccc gcgtcccact actgggactt ccgtgaggcg
     2221 cgctgggtcg tgtgccgcgt gccggaggtg gcggcgcccg accccgcctc agtggcgagg
     2281 tccgaaccgg ccgctgagcc gctcgacgcg gccggccgcc cgaccatccc ggcccagggc
     2341 ggcgaccgcc ggcttccctc gcggcacgga tagggccccg caccgtcgac gcgccggccg
     2401 cggtggcctc ctgacggtcc cgggccgggt cctgccagac ttcgccccat gcgcgtgcac
     2461 atcggctgcg accatgccgg cttcgagctg aaggaacacc tcgtcggctg gctcgaggac
     2521 cagggtcacg aggtggtcga ccacggcgcc cctgcctacg acggtcagga cgattacccg
     2581 ccctactgcc tgcgcgcggc cgaggccgtc gtcgccgacc gtggcagcct gggcatcgtg
     2641 ctcggtggct cgggcaacgg cgaggcgatc gcggccaaca aggtgcgtgg ggtgcgggcc
     2701 gcgctcgcct ggaacgagga gatcgcccgg ttgggccgcg agcacaacga cgccaacgtg
     2761 gtgagcctcg gggcccggat gcacaccgtc gacgaggcga cccgcatcgt cgcggcgttc
     2821 ctcgacaccg tgttcagcgc cgacgagcgc cacgcccgcc ggatcgcgat gctgacgcac
     2881 tacgaggaca ccggcgaggc gcctgccgct cccggcgtcg cctgagcggc cggggccggc
     2941 gtgccggagg ggcacaccat ccaccgcctg gcgcgtgagc tgggcacccg gttggccggt
     3001 gggccggtgc gcgcctgcag cccgcagggc cggttcgccg ccggggcggc ctcgatcgac
     3061 gggcacccgc tggcccgggc cgaggcgcac ggcaagcacc tgctggcccg gttcgaccac
     3121 cagcgctcgc ggttgtgggt gcacgtgcac ctgggcatct acggacggct gacgtttggg
     3181 caggcgcccg caccggcgcc gatcgggctg gtccggctgc gcctggaggg tccgcgcgcg
     3241 tgggccgacc tgcgcggtgc caacacctgc gagctgctcg acgccgccgg acgcgacgcg
     3301 ctgatcgccc ggctcggccc cgacccgctg cgccgcgacg ctgaccccga ccgagcctgg
     3361 gtccggatgc agcgcagccg ggcgccggtg gcgacgctgc tgatggacca gtcggtgcta
     3421 gcgggggtgg gcaacatcta ccgcgccgag atcctcttcc ggcatggcgt ggacccgatg
     3481 ctgcccggcc gcgcgttggc gcgtgaggtc tgggacgcga tgtgggccga cctgcaggtc
     3541 ctcatgcggg ccggcgtgcg gaccgggcgc atcgacacgg tgcgccccga gcacgaccct
     3601 cgggtgacgg gccgggcgcc gcgccgggac cggcacggcg gagaggtcta cgtctaccgc
     3661 cgggccggtc agcactgcca tgtctgcgcg accccggtcg ccagcgcggt gctcgccgga
     3721 cgcaacctgt tctggtgccc gcgctgccag ccgccggggg cgacgggcgc ggggctcgcc
     3781 gcccgcgtct gagcgccggg gcgtgtccgg tcgcaacccg cccgatcggc ctccgcacgg
     3841 atccgatgat cttgggacag aatgagtgtt ttgtcccctg caggagagtt ggcgctcgac
     3901 atggcagctg ccggccagcc gatggtcgcg ggtgctgcgt ctgacgggtc gcccggccgg
     3961 ctcgccgcgg tcgtgcctgc ccggtcgccc cggtcctcgc cggcccgtcg cgtgcgccgg
     4021 ctgtggcgga ggatctccca cggtcgtcgc ttgcgggcgc tgcgctctga cggcgcattg
     4081 ttggtggcgc tgacgctggc gagcgtcggg ctgtgctggg ccgggaccgc gtggcctcgg
     4141 ctgttccccg agtcgacgct ggctctcgtg gtgctcgccg gcggcctgat gctgcgccgg
     4201 cgcagcctgc tgctgctcca cgtcgtggtg gccgccacgc tggcctacgc cgtcgtcggg
     4261 cggcccagcg tgggcatcgg ttcggtcggc gtcgtcgtgg tgaccggagc cgccgtactc
     4321 gtgctctccc gcaccaggac ccggctcggg gtgccgggca cgtcgggaga gacgatgctc
     4381 gtcgacctgc gcgaccggct gcgtcagcag ggcgagatgc cgccgctgcc cgcggggtgg
     4441 cacgccgaga tcgtgctgcg accggccggc ggagactcct tcagcggcga cttcctcgtc
     4501 gcgtcgcggc cgggcgacgg ggccacgttg gaggtcgggc tcgtggacgt gtcgggcaag
     4561 ggacgctcgg ccggcacccg ggccctgctg ctctccgggg cgttcggtgg actgctcggc
     4621 tcgatggcgc cgggcggctt cctgcaggcg gccaactcct acctgctccg ccaggactgg
     4681 gacgaggggt tcgccacggc ggtgcacgtg gtggttgacc tcgcctccgg ggagtacgtc
     4741 gtccgctccg ccgggcaccc gccgatcgcg cagttcgcgg ccgggtcggg tcgctggagg
     4801 ctgcgtgatg ccgcgcgcgg cccggcgctc ggggtcatcc ccggcgcgac ctatctcggt
     4861 gagacaggtc ggctcggtcg gggagacgcc ctgctgctct acaccgacgg gctggtcgag
     4921 gtgcccgggc gcgacctgtc gctgggcatc gaccggttgc tcgggcacgc cgagcggctg
     4981 gtcacccgcg gctttcgcca cggcgcccgc acgttgatcg actcggtcgc gtccgggcgc
     5041 agcgacgacc gagcgctggt cctcatctgg cgcacctagc cgcgccgtcg gtcctaccgt
     5101 gtccccatgc gaccggtgca cttcgagatc cacgcctcgg acccgaccgc ggtccgcgag
     5161 ttctaccaga gcgtgctggg ctggcggttc cagcagtggg gggacaaccc ctactggatc
     5221 gtgatcaccg gcgacggcga tccgatggcg ggggtgccgc acagccagcc cggggtcgac
     5281 ggcgggctgc tgccgcgcct gggtggccgg cccgaggaag gtcagccggt caatgcgttc
     5341 gtgatgaccg tcgaggtgcc tgactgcgac gcctacgtcg acaaggccgt cgcggccggc
     5401 ggttcggtgg ccctgcccgc caacgacatg gccggggtgg gccggctcgc ctacgtcaag
     5461 gatcccgacg gcaacctctt cggcatgctc gagcccgctc ccgtggagga ggcccccaac
     5521 ggctcatgac ctgccggggt cgtcgccccc cgacgtcgag gagtctgcct cgcctggccg
     5581 tggctccatg tagatgagct cccagaggtg gccgtccagg tcctggaacc cccagccata
     5641 catgggccct tcttggacgg gctccttgta gcgcaccgct ccggcaccga gcgcggcgtt
     5701 gacgagctcg tcgacgcgct gcttgctctc ggcgctcagc gcgatgatcg tctcggtgct
     5761 gcccgtcgag tcggcgacct tcttcggggt gaacgtggcg aagaagtcct cgcgcagcag
     5821 catgacattg atggactcat ccaccacgag gcacgcggcg tccttgttgg tgaactgcgg
     5881 gtcgaaccca tatccgagct tcgcgaagaa tgccacggat ctgtccaggt cccgcaccgg
     5941 cagattcacg aagatgcttg tcgacatgcc tgcacgttaa gcgcgtgcgg cggtccacgc
     6001 cctggtctcg gcgatttgcg gcgactagcg tgaagcggtg tctgagccgg tgaaggcccg
     6061 cagcgccctt cggctccggg ctgtcatgtc attgatcgcc ctgcccgtcg cgttgatcgc
     6121 cggggtgatc ctgctggcgg tcgccccggg gtcgggcgag gaccgggcgc cttatgtcgt
     6181 ggccgccatc atctgtttcg ccgtggtcgt catcgccgcg gtcgacctcg tcgtcattgc
     6241 tcggcgggtc aagcaagagc cgcgtcgacg cggctgagcg ggctcgcgcg tgcaccaggc
     6301 tcggctggca acgggggtgc gcctgccctt tgtcgtcacc ggtgacccgt cggggccgcc
     6361 tctgctgctg gttcacccct ggggtgaggc gcacggatgc ttcgaccggc tggccccgct
     6421 cttgccgcag tcgtggcatg tcatcgcgat ggaccagaaa ggtcacggcc gggcaagcaa
     6481 gccagcggcc ggctatgcgc ttgtcgacct cgcctccgac ctggcggcgt tcctcgacgc
     6541 gctcgagatc acccgggccg tggtcctcgg ctcctcgagc ggggggtacg tcgcacaaca
     6601 gctggcggtc agccatccgg agcgggtcgc ggggctcgtc ctggtcggca cgccgcgcag
     6661 cctgcggggg cggccggcgt tcgccgccga ggtcgagggg ctcaccgacc cggtcgaccc
     6721 cgagtgggtc cgtcagtcgc tgagctggtt tcccaccatg gcgccggtgc cctcctggtt
     6781 cgtcgctgag cgggtggctg atgggctgcg ccttccggcg cgcgtgtggc ggcaggcgct
     6841 ggccgggctc tgtgaggccg tgccgcctac cgaggtggcg accatcacgg cgcccacgtt
     6901 ggtcatctgg ggcgctcgcg acgggctatt gccgcgggcg gatcaggagg ctctcgtcgc
     6961 ggccatcccc gactcgcgcc tcatcgtcta cgcggacacg ggacacctgg tgctgtggga
     7021 gcaacctggc cgagtggccg ccgacacggc tgccttcgtg aagggttgcc cgggctgaga
     7081 cctgagcctt gagccggttg acgaccagcg ccaccgcgac cacgagaaat gctcccgcga
     7141 ccgcgtcgag gagatagtga ttggccgtca cgaacaccac gagacaggtc gcaaggggat
     7201 agagccacac gagacgagtg atcagacggt ggccaatgac gcaggcctgg accgccagcg
     7261 cgcaccacat ggcccaggcg acgtgaaggc tcggcatcgc ggcgtagagg ttgacgaacg
     7321 cgctgggccc atgggggtcg gcggccgaga ggatgttctg ctccaccagc acgtcgtgca
     7381 gcccgaagct gtcgatgagc cgcggtggtg cgaccggata g
//
LOCUS       DATIBB010000182         4565 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_246128, whole genome shotgun sequence.
ACCESSION   DATIBB010000182 DATIBB010000000
VERSION     DATIBB010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 4565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4565
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_246128"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..405
                     /locus_tag="VLC50_07330"
     CDS             <1..405
                     /locus_tag="VLC50_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009716711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asd/ArgC dimerization domain-containing protein"
                     /protein_id="HSF27312.1"
                     /translation="DGWSSEELKVRNESRKILGLPDLRVAATCVRVPVITTHSLAVHA
                     VFEREVDAAAAQEILRDAPGVVLFDDPAAGEFPTPADVVGTDPTWVGRVRRSIDDPTA
                     LDFFVCGDNLRKGAALNTAQIAELVAAELTSA"
     gene            complement(420..713)
                     /locus_tag="VLC50_07335"
     CDS             complement(420..713)
                     /locus_tag="VLC50_07335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF27313.1"
                     /translation="MAGKEPDDIERLLAEVDQSLSGRRTPARKGEASAARPAKDDDSS
                     ALTVAVVAGVIGAVMVLLLFAVLPFLGAYSGAAGAFLGAAGATLVVGWLHRRR"
     gene            complement(778..2610)
                     /locus_tag="VLC50_07340"
     CDS             complement(778..2610)
                     /locus_tag="VLC50_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016473075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HSF27314.1"
                     /translation="MASSPDPAAAATAPQPKWEQRFRAVRVSLPDWAQDAPARTVYVS
                     NATGTFEVYAWDRASGSHRQVTARRNGTSHATIDPSGQWIWWFDDTDGDEFGVWRRQP
                     FGGGPDAPAAAPLSASYPAGLGLGPDGLAVVGRATEGRTTIHRVRADAEPVTLYEHTE
                     DASVGALSRDGALVAIEHSEHGDSRHPALRVLRVADASAVAELWDGPGKGLEALEFAP
                     VPGDPRLLVQHERHGRPELLIWDVLAGTQTELTVDLPGEISAQWYVDGRALLVAHDFE
                     ARTTLHRLDLDTGELTDLGTPPGVVDDATTRPDGSVEYLWSSAATPPAVRSTTGRVVL
                     ATDGPPAPPSVPVEDAWVDGPAGRIHALVSRPASDADSTSAARAPYPTVFLVHGGPTW
                     LDSDAFAADVAAWVDHGFAVVRVNYRGSIGYGSAWRDALEGRVGLTELEDVAAVRAWA
                     VESGLADPARLVLAGGSWGGFLTLLGLGTQPDLWTVGVAAVPVADYVAAYDDEMDALQ
                     AFDRSLFGGSPEQVPERYRESSPITYVDQVRAPVLVLAGANDPRCPIRQIDNYLAALA
                     GRSHPHEVYRFDAGHGSLVVDERVRQMRAELDFALRHLPPAKRA"
     gene            2682..3047
                     /locus_tag="VLC50_07345"
     CDS             2682..3047
                     /locus_tag="VLC50_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007300330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HSF27315.1"
                     /translation="MTDTPDAVYPAATREIGARRRELAPGIHEAFDNFSKAVFAEGAL
                     PEKTKQLIAVAVAHVTQCPYCIKGHTKVAMRKGATPEEIMEAVWVAAEMRAGGAFAHS
                     TLAITEMDRAAHNGHAAVQ"
     gene            3099..3518
                     /locus_tag="VLC50_07350"
     CDS             3099..3518
                     /locus_tag="VLC50_07350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF27316.1"
                     /translation="MPERRAKLAAAGRPVASLVTLTLAATASLVLAFVPMGTSESVTV
                     SNDGPVSRTVASTTMLQTEGPSVLVALALPVLLAALPVLVPPRYRLVVAVPSAVLLGV
                     FVFLAMFSVGIFFLPAFIASVVAAARLRSSGQPTPAR"
     gene            3579..4298
                     /locus_tag="VLC50_07355"
     CDS             3579..4298
                     /locus_tag="VLC50_07355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF27317.1"
                     /translation="MHHTRVQTTTLLIAAVAAALTVAAPLPQRLSVSTVAAAPDVPAT
                     QDRPDVAAPRAGDPISAAEMRDLQTVAEQSGTPLGEVIERYAWNDSFALAVDQLREAA
                     PEHFAGAAIVDAHHAWIAFKGAQPPVMRTLAATFERISGDVVVAIRADVGFSEREVDA
                     AVAAAHFAVLTSAGVGDATTSFDFDTRQIIATVTPSMTSVILDAGALKDVASRAVHEV
                     VADGELSRVAITVRVAGPATR"
     gene            4325..>4565
                     /locus_tag="VLC50_07360"
     CDS             4325..>4565
                     /locus_tag="VLC50_07360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF27318.1"
                     /translation="MVGVIALLVAAGLVVTRGFVWRARVEVAHASLSPEGALLLHVDT
                     CGGDPVIDLIEETSDEVRVAVVSTRRMFRGGGDCRD"
BASE COUNT          632 a         1651 c         1632 g          650 t
ORIGIN      
        1 gacggctggt cgagcgagga gctcaaggtc cgcaacgagt cgcgcaagat cctcggactg
       61 cccgacctgc gggtggcggc cacctgcgtg cgagtcccgg tgatcaccac ccactcgctg
      121 gcggtccacg cggtcttcga gcgcgaggtc gacgcggctg cggctcagga gatcctgcgc
      181 gacgcgcccg gggtggtcct cttcgacgac ccggccgcgg gggagttccc gacgccggcc
      241 gacgtcgtgg gcaccgaccc gacctgggtg ggccgggtac gtcgctcgat cgacgacccg
      301 accgcgctcg acttcttcgt gtgcggcgac aacctgcgca agggcgccgc gctcaacacc
      361 gcccagatcg ccgaactggt cgccgccgaa ctgacctccg cctgatcgcg cgggcccgct
      421 cagcggcggc ggtgcagcca gcccaccacc agcgtcgccc cggccgcgcc gaggaacgcc
      481 ccggccgccc cggagtaggc gcccaggaac ggcagtacgg cgaacagcag cagcaccatc
      541 accgcgccga tgactccggc gacgaccgcg acggtcaggg ccgacgagtc gtcgtccttc
      601 gcggggcggg ccgcagatgc ctcgcccttg cgggccggcg tcctgcgccc gctgagcgac
      661 tggtcgacct cggccagcag ccgctcgatg tcgtcgggtt ccttgcctgc catgccccca
      721 tcctcaccca ggccgggggc cacgccaagc cgttcgaccc gccaacccct gcactgatca
      781 tgcgcgtttg gcgggcggca ggtgccggag ggcgaagtcg agctcggcgc gcatctgcct
      841 gacccgttcg tcgacgacaa gtgagccgtg cccggcgtcg aaccggtaga cctcgtgcgg
      901 gtggctccgg ccggccagcg cggccaggta gttgtcgatc tggcggatcg ggcagcgcgg
      961 gtcgttggcc ccggccagca cgagcaccgg cgcgcgcacc tggtcgacat aggtgatcgg
     1021 cgaggactcc cggtagcgct cgggcacctg ctcgggcgag ccgccgaaca gcgagcggtc
     1081 gaaggcctgc aacgcgtcca tctcgtcgtc gtacgccgcg acgtagtcgg ccaccggcac
     1141 cgcggccacc ccgaccgtcc acaggtccgg ctgggtgccc agcccgagca gggtgaggaa
     1201 cccgccccac gagccgccgg ccagcaccag acgagccggg tcggccagcc ccgactcgac
     1261 cgcccaggcc cgcaccgcgg cgacgtcctc gagctcggtc agcccgaccc gcccctccag
     1321 cgcgtcgcgc caggccgagc catagccgat cgagccgcgg tagttgaccc gcaccaccgc
     1381 gaagccgtgg tcgacccagg cggccacgtc ggcggcgaac gcgtcggagt cgagccaggt
     1441 cggcccgccg tgcaccagaa agacggtcgg gtacggcgcc cgcgcggcgc tcgtgctgtc
     1501 cgcgtcggag gcggggcggg acaccagggc gtggatgcgc ccagccggcc cgtcgaccca
     1561 ggcgtcctcg accggcaccg acggcggcgc cggcggaccg tccgtggcca gcacgacgcg
     1621 gccggtcgtc gagcgcaccg cgggcggcgt ggcggccgag gaccacaggt attcgaccga
     1681 cccgtccggg cgagtggtcg cgtcgtcgac gaccccgggc ggggtgccca ggtcggtgag
     1741 ctcgccggtg tcgaggtcga gccggtgcag cgtcgtacgg gcctcgaagt cgtgcgcgac
     1801 cagcagggcc cgcccgtcga cgtaccactg ggcgctgatc tcgccgggca ggtccacggt
     1861 cagctcggtc tgggtgccgg cgagcacgtc ccagatcagc agctcgggcc ggccgtgccg
     1921 ctcgtgctgg accagcagcc gcgggtcgcc gggcaccggg gcgaactcga gcgcctccaa
     1981 ccccttgccg ggcccgtccc acagctcggc gaccgcgctc gcatcggcca cgcgcagcac
     2041 gcgcagcgcc gggtggcgcg aatcgccgtg ctcgctgtgc tcgatggcca ccagggcacc
     2101 gtcgcgggag agggcgccga cgctggcgtc ctcggtgtgc tcgtagagcg tgaccggctc
     2161 ggcgtcggcg cgcacccggt ggatcgtcgt acgcccctca gtggctcgcc ccaccacggc
     2221 cagcccgtcg ggtcccagcc cgagcccggc cgggtacgac gcagatagcg gtgccgccgc
     2281 cggtgcgtcg gggccgcccc cgaaaggctg gcgccgccac accccgaact cgtcgccgtc
     2341 ggtgtcgtca aaccaccaga tccactgccc ggacgggtcg atcgtcgcgt gtgaggtgcc
     2401 gttgcgccgc gcggtgacct gccggtggct gccgctggcc cggtcccacg cgtagacctc
     2461 gaacgtgccg gtcgcgttgc tcacgtagac ggtgcgcgcg ggtgcgtcct gcgcccagtc
     2521 gggcaacgag acgcgtacgg cgcggaagcg ctgctcccac ttcggttggg gcgccgtcgc
     2581 agccgcggca gggtcgggcg aagaggccat ggtggtcatc caaacacgag ggggaggatg
     2641 ctgaagacgc tgatgtgccc ttcgaacgga gatgtgcagc aatgaccgac acgcccgacg
     2701 ccgtctaccc ggccgctact cgcgagatcg gcgcccgtcg ccgcgagctg gccccgggca
     2761 tccacgaggc gttcgacaac ttcagcaaag cggtgttcgc cgagggggcg ctgccggaga
     2821 agaccaagca gctgatcgcg gtcgcggtcg cccacgtcac gcagtgcccc tactgcatca
     2881 agggccacac caaggtcgcg atgcgcaagg gcgccacgcc tgaggagatc atggaggcgg
     2941 tctgggtggc cgccgagatg cgcgccggcg gtgcgttcgc gcactccacg ctggccatca
     3001 ccgagatgga ccgcgccgcc cacaacggcc acgccgcggt gcaatgacca cgccacgtgg
     3061 gtgttctggc gcttggcagg gtgtgcgagc ctagccaggt gccagaacgc cgcgccaagc
     3121 ttgctgccgc cggccgtccg gtcgcctcgc tcgtcacgct gactctcgcg gccacggcct
     3181 cactcgtgtt ggcgttcgtg ccgatgggca cgagcgagtc ggtgacggtg tccaacgacg
     3241 gccccgtctc gcggacagtc gcatccacga caatgctgca gaccgagggg ccgtcggtgc
     3301 tggtggccct cgcccttccc gtgctgctgg ccgcactgcc ggtgctggtg ccgcctcgct
     3361 accggctggt ggttgcggtg ccctccgccg tgctgctggg ggtgttcgtg ttcctcgcga
     3421 tgttcagcgt cgggatcttc ttcctgcccg cattcattgc atcggtcgtg gccgcggcgc
     3481 ggttgcgatc gtcaggccag ccgacgcctg cccgctaggg atactctggc agcgccgcag
     3541 ggtttcggca tcgacgcgtg ctgttggcag gggggccgat gcaccacacc cgggtgcaga
     3601 cgaccactct tctcattgcc gcagtcgccg ccgcactgac cgttgcggcg ccgttgcccc
     3661 aaaggctgtc ggtgtccacg gtcgccgctg cgccagatgt ccccgcgact caggatcgtc
     3721 ccgacgttgc cgcgccacgt gccggcgatc cgatcagcgc cgcagagatg cgcgacctgc
     3781 agaccgtcgc cgagcagagt ggcacgcccc tgggcgaggt gatcgagcgc tacgcctgga
     3841 acgacagctt cgccctggcg gtggaccagc tccgcgaggc agccccggag cactttgccg
     3901 gggcagccat cgttgacgcg catcacgcgt ggatcgcctt caagggtgct caacctccgg
     3961 tgatgcgcac cctggcggcg accttcgagc gcatttcagg cgacgtggtc gtcgccatcc
     4021 gggccgacgt cgggttctcc gagcgtgagg tcgacgccgc ggtcgcggca gcgcacttcg
     4081 cagttctcac atctgccggt gtgggtgatg cgaccaccag cttcgacttc gacacccgtc
     4141 agatcatcgc gacggtgacc ccgtcgatga cgagcgtcat cctggacgca ggagcgctga
     4201 aggacgtcgc atcccgggca gtgcacgaag tcgtggccga tggcgagctc agtcgtgttg
     4261 cgatcacagt ccgggtggca ggaccggcga cgcgatgagg cgccggcggt tggcaatcgc
     4321 ggccttggtc ggggtcatcg cgctgctggt cgcggcgggg ctggtggtga cgcgaggttt
     4381 cgtttggcgg gccagggtcg aggtcgcaca cgcctcgctg tcgcccgagg gcgccctcct
     4441 cctgcatgtg gatacctgcg gtggtgaccc cgtcatcgat ctcattgaag agaccagcga
     4501 cgaggtgcgt gttgccgtgg tctcgaccag gcggatgttc cgcggcggcg gcgactgccg
     4561 agaca
//
LOCUS       DATIBB010000183        10843 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_1035759, whole genome shotgun sequence.
ACCESSION   DATIBB010000183 DATIBB010000000
VERSION     DATIBB010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 10843)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10843)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10843
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1035759"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..489
                     /locus_tag="VLC50_07365"
     CDS             <1..489
                     /locus_tag="VLC50_07365"
                     /inference="COORDINATES: protein motif:HMM:NF025063.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HSF27319.1"
                     /translation="PPGLIHHSDRGAQYTAFSFGQHLIDAGIAASMGSKGDAYDNAMM
                     ESFFGTLKIELIDRRRWPTRGEVEIALMSYIEGWYNPRRLQEALGWRSPLEYEAAHHA
                     GHDLSVPATCQPAPALAVVKAKPSGRPPAGLDPGYGQAPNGNGGATGGPQPKPADRVG
                     TT"
     gene            774..846
                     /locus_tag="VLC50_07370"
     tRNA            774..846
                     /locus_tag="VLC50_07370"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:807..809,aa:Arg,seq:acg)
     gene            complement(933..1595)
                     /locus_tag="VLC50_07375"
     CDS             complement(933..1595)
                     /locus_tag="VLC50_07375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF27320.1"
                     /translation="MTTLTRVLGLGSAAAFAGFPLVRPWGDKSSDPAAMVDAFASSEW
                     VIAHSLGMIAWVLLAATLVSLARADAGSRLTAWVAGIGTAAILPYYGAETFGLHGLAV
                     AANTDSDISLVSTAQESIRGGAAAATCFGVGLLLLAAAGVAIATTTWRSGTSLRWLGV
                     PVGLGLLTYLPQFFTPNVIRQAHGVALALTLALWLLLREDSPATPAPAQAQVAAKAPT
                     GV"
     gene            1838..2161
                     /locus_tag="VLC50_07380"
                     /pseudo
     CDS             1838..2161
                     /locus_tag="VLC50_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003948472.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
     gene            2238..4142
                     /gene="nhaA"
                     /locus_tag="VLC50_07385"
     CDS             2238..4142
                     /gene="nhaA"
                     /locus_tag="VLC50_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016827489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HSF27321.1"
                     /translation="MAAAASDQTRLGRRDLLDQLSGPLRRYLATESGGAGLMLGAALV
                     ALIWANSPWSSFYESLWGTQASVSLGGAELSMDLQHWVNDGLMALFFFVVGLEVRREI
                     SMGELNDRTRVVVPLIAGIGGMVVPALLYLVVNSSGEAAAGWGIVIGTDTAILLGVLA
                     LVGPTFSTQLRVFLLAITVVDDIVAVTIIGVVYSDSINPLPLAIALAGLAVLPLLNRL
                     GVWQASVYGVVVVVVWLSTVESGLHASIAGMAAGLLVAAFPPRRADVEHAASLFRAFR
                     QSPAPGVGRTAKRGLQRAVSVNERLQANLHPWVSYVIVPVFALANAGVDLRGGTLGEA
                     LRSPITWGVVLGLVVGKFLGISAAALATVRFTIGALPHGVTRGQVFGGAALSGLGFTV
                     SLLIASLAFDSEALREQATVGVLLAAMLSAATGWLAFRLEGILGGAAAARPLDLHPPV
                     EIGHDHIRGPVDAPLTLVEYGDFECPFCARATGAARELRAILGDDLRYVFRHLPLSDV
                     HPNAEQAALAAEAAAAQGRFWQMHDLLFENQDQLETEDLIGYAADLDLDVEQFVRDLD
                     DDELAARVRDDVAGAEASGARGTPTFFIGGRLYVGPHDAASIAAALRTAGGGTEPAVT
                     NPSTTGSVDG"
     gene            4135..6186
                     /locus_tag="VLC50_07390"
     CDS             4135..6186
                     /locus_tag="VLC50_07390"
                     /EC_number="3.6.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005254338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005375 - copper ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0043682 - P-type divalent copper
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HSF27322.1"
                     /translation="MDEHRHADHAQAEHAHGEHDKHAGHSVDMFRRRFWRSLLLTIPV
                     VATSEMVMDWFGYRLDFPGLDWVGPLLGSVIFFWGGWPFLEGGRREVMARQPGMMLLI
                     SMAITVAYVASMATSLGWFELDFWWELSLLVTVMLLGHWQEMKALGQAQDALAALAAL
                     LPDTASRVGPGDEIVTVGLADLRAGDVVLVRPGERVPADATVLEGSAELDESMITGES
                     RAVTKSAGDTVLAGTVTTDNALRARVTAVGEGTALAGIQRLVSDAQASSSRAQDLADR
                     AAAVLFYVAAGVGLLTFVVWTLLGEVREAVTRTVTVLVIACPHALGLAIPLVIAIATE
                     RAARAGVLVRNRLALERMRTVDVVLFDKTGTLTKGEPVLTQASGTNGTPADEVLALAA
                     AVETDSEHPVARAIVQAAGERVPAGAHRSRATDFRASPGRGVQARVGASTVAVGGPAM
                     LTAEGLAVPAELAAATVPWVRRGASVLYVVRDGALVGALALEDAVRAESAQAIDALHA
                     RGLRVAMITGDARRVAESVAASLRIDEHFAEVLPQDKAASVAALQARGHHVAMVGDGV
                     NDAPALAGAEVGIAIGAGTDVAIESADLVLASSDPRAVLSAIELSRASYRKMQQNLAW
                     ATGYNVVAIPLAAGVLEPVGVSISPALGAVLMSVSTIVVALNAALLRRTDLDPARLAA
                     P"
     gene            6252..6563
                     /locus_tag="VLC50_07395"
     CDS             6252..6563
                     /locus_tag="VLC50_07395"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007929407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HSF27323.1"
                     /translation="MADIKVRNHAEEQRYEAWVDGRLAGVAGYQTTHDLIVFTHTDVE
                     PEFKGKGIGSVLVRRALDDVRAEGTHRVLPLCPFVKSWVANHREYADLLYSSPRSTAT
                     D"
     gene            6583..6915
                     /locus_tag="VLC50_07400"
     CDS             6583..6915
                     /locus_tag="VLC50_07400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF27324.1"
                     /translation="MAPSAVTVGPCRFALRADGDKPGMYVVEILDHLGDPAQFVVAPI
                     VALDLALRAQRAGLRWAVNDEASARPVSLEDLRHQARRLAATDTAVAEALEEAERAHE
                     RGLRATLN"
     gene            7146..9758
                     /locus_tag="VLC50_07405"
     CDS             7146..9758
                     /locus_tag="VLC50_07405"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HSF27325.1"
                     /translation="MPPAKVATPRLPALFVPRPRLHGLLTEAVASRVTLVSAGPGSGK
                     TLGVASWAADGRAPGPVAWLALDESDNDPATLWREIVAALHVAGAPASRAGVLHVSPF
                     GAPEIRRLTAGFTSSAAPVVLVVDDVEALTRDEARACLDQLIAEASAQLRIVLITRTD
                     PDLPLHRLRVVGDLSEIRGDELAFDGEEAGRLFADRGLSLDAGQRESLLARTEGWATG
                     LRLALLALSDGDVDRAISDFSGDQRSVADYLIGEVLRRRPPQVQRLLLRTSITERICG
                     PLADLLADRTHSQRELERLAQAHPLVHSLDAHHTWFRYHRLLRELLQHELQIQEPSLV
                     PELHRRAAAWWEAAGQPVEALRHASAAGDWRHVRRLLVDVALPMLLSPDRSTLAGAVR
                     PAAAQAAVRPSLDTLIPAAAWHLFERNIPVMDKDADEAERRLGELDDAEVPMARVAIG
                     LFRLMALRTSGDANEVVRVAGEMLSLLRLGPGSRPPAARHYEVVAAAHLAAGLLWLGE
                     HAQARRHLVTAAAEADALGLGLTRLFAHSHLAVLDAMEGRLHAAEQTASAVLDSAARG
                     GWGDESQVQAARLAIAMVHLARDRPDLAGRQLGEGLDGTGSADGAVRLAMRIALVHVA
                     LAHRDPSSARLACSRIRDEITVSAAPDWVLGWLATTEAETALLTNNAELALTLLKSVP
                     HSPEAQVLRVRAHLLLGDRVGASDALALLLARLPVSLAVHVQVWLLEGVLANARHDDV
                     SAIEALRRALALAEPEELRRPFRALGPDLAATVARYLDVVEGLGAFALRHGDGGQAEG
                     GPEDRLLLAERLSSRELAVLSYLPSMLTNEEIGMALFVSVNTVKAHLKSLYRKLGVTG
                     RREAVRRARELDLL"
     gene            9853..10338
                     /locus_tag="VLC50_07410"
     CDS             9853..10338
                     /locus_tag="VLC50_07410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017834123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HSF27326.1"
                     /translation="MATLTAWRFDTPDGAEEAVETLESLAKQRLITLHDAATVTWEPD
                     KKKPKTRQLNNLVGAGALGGAFWGLLFGLIFFVPLLGLAIGAGMGALGGSLTDVGIDD
                     DFIRKARDEITPGTSALFVMTSDAVEDRVREAFSGHKAHLMFTNLSADQEKALRSVFS
                     E"
     gene            10540..>10843
                     /locus_tag="VLC50_07415"
     CDS             10540..>10843
                     /locus_tag="VLC50_07415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF27327.1"
                     /translation="MDGVAGTLGGAVEQITAGLARVDDAAFVRASDRELLAAVPVLES
                     LTARLEWVRAHVVGEVDARGLATEAGACSTPAWLAGVSRAHPGSTSRLVGLARALPR"
BASE COUNT         1442 a         3844 c         3968 g         1589 t
ORIGIN      
        1 ccgccggggc tgatccatca cagcgaccgc ggagcccaat acaccgcgtt ctccttcggc
       61 cagcacctca tcgacgccgg cattgcggcc agcatgggca gcaagggaga cgcctacgac
      121 aacgccatga tggaaagttt cttcggcacc ctgaagatcg aactcatcga ccggcgtcgc
      181 tggccaaccc gcggcgaggt cgagatcgca ctgatgtcct acatcgaggg ctggtacaac
      241 cctcgccgcc tccaagaagc cctgggatgg cgcagcccgc tcgagtacga ggccgcccac
      301 cacgccggcc acgacctgtc ggtgcccgcc acctgccagc ctgcgcccgc cctcgccgtc
      361 gtcaaggcca agccctccgg gcggcctccg gccggccttg accccggcta cgggcaggcg
      421 cccaatggca acggcggcgc caccggcggc ccacaaccga aaccggccga ccgggtcgga
      481 accacctaac cggtctccaa gaaagcgggg gaggccctcc ctgctgtgcg actaccacca
      541 cgaccaagcc caccgacaag gctggcggtc acgtctctcc tcagccaacg ggcaggtcga
      601 atggcagcca ccgagagcgc tcgaccgcgc ccagcagtgg cggcagcacc accgctacac
      661 cctcgacacc atcagacgca gatagaccgg gccgcctgat gcgatcggcg gtgggagggg
      721 ttcgtgcggt cgcggcctcg gctgctagcc tctatgggcc ggttagccgg catgcgctcg
      781 tagctcaacg gatagagcat ctgactacgg atcagaaggt tgggggttcg agtccctccg
      841 agcgcacagt gcctgacctg cgaagagacc cccaccggct gcacgccagt gggggtctct
      901 cttgcggtac acgccggtgg cggccttcgc ggctacacgc cggtgggcgc cttcgccgct
      961 acctgagcct gggccggcgc gggcgtcgct ggcgagtcct cgcgcaagag gagccacagc
     1021 gccagggtga gcgcgagggc gacgccgtga gcctggcgga tgacgttggg cgtgaagaac
     1081 tgcggcaggt aggtgagcag gcccagcccg accggaacgc ccaaccaccg cagcgacgtc
     1141 ccggatcgcc aggtggtcgt cgcgatagcc acacctgccg cggccaacag gagcaggccc
     1201 acaccgaagc acgtcgcggc cgcggccccg ccgcggatgg attcctgcgc cgtggagacc
     1261 aagctgatgt cgctgtcggt gttggccgcg acggcgaggc cgtgcagtcc gaaggtctcg
     1321 gcgccgtagt aggggaggat cgcggcggtg ccgatccctg cgacccacgc ggtcagacga
     1381 gacccggcgt cggcgcgagc cagcgacacc aacgtggcgg ccaggagcac ccaggcgatc
     1441 atccccagcg agtgagcgat gacccattcc gaagaggcga aggcgtcgac catcgccgct
     1501 ggatcgctcg acttgtcacc ccaagggcgg acgaggggga atccggcgaa cgcggctgca
     1561 gagcccagcc ccaggactct cgtcagggtc gtcacggctg aaggccggca tgcgcgccga
     1621 agtcgacccg gatagggacc gggacccccg tctgggttgg ggactcagtg atcatcatga
     1681 gacgaccata cgacgtgtgc gacgcagcgt atggtccatt tttggcccga atgggctggg
     1741 ccacccgggg ttctcgacct gcacctggac ccacgcgggc gcggccgacg ggcggggctc
     1801 gaggccgcgc tgcgcgtcgc cgtacgtcgt ctgtgccggt gccgcgagca agagcctggc
     1861 gcccgggctc cggctgggct ggctcgtgct gccggccgac cttgtcgaat cggtgcttgc
     1921 gatcaaggcc gccgccgacc tgcacagcag cacgctcgac cagctcacgc tcgccgcctt
     1981 cctcaagagc ggcgactacg accggcacgt acggtggtcg cggctggcct accgccgtcg
     2041 ccgtgaccgg ctcgtcacgg cgctcgggag ggcggcaccg accgcgcggg tcaccgggat
     2101 cgcggccggc ctgcacgtgc tggtcgagct gccccccggg ccgaccgagg gcgaggtggt
     2161 ggcgcgggca cgcgttcacg acggcggtcg cgcggttgtg cgccgtactc caagaatgcg
     2221 gcccgtaaag tgggcgaatg gcagccgcgg cctcggacca gacccgactc gggcggcggg
     2281 acctgctgga tcagctctcc gggccgctgc gcagatatct ggcgactgag tcgggcgggg
     2341 ccgggctcat gctcggcgcc gctctcgtgg cactgatctg ggccaactcg ccgtggtcga
     2401 gcttctatga gtcgctgtgg ggcacccagg cgtcggtgag tctgggcggc gccgagctgt
     2461 cgatggacct gcagcactgg gtcaacgacg ggttgatggc gctgttcttc ttcgtcgtcg
     2521 ggctcgaggt gcgtcgcgag atctcgatgg gcgagctcaa cgaccggacc cgggtcgtcg
     2581 tgccactgat cgccggcatc gggggcatgg tcgtcccggc gctgctctac ctggtcgtca
     2641 actcctccgg tgaggccgcc gcgggctggg gcatcgtcat cggcaccgac accgcgatcc
     2701 tgctcggggt gctcgcgctg gtggggccga cgttctccac ccagctgcgg gtctttttgc
     2761 tcgcgatcac ggtggtcgac gacatcgtcg cggtcacgat catcggggtc gtctactccg
     2821 actcgatcaa cccgctccct ctcgcgatcg ccctggctgg cctggcggtc ctcccgctgc
     2881 tcaaccggtt gggcgtgtgg caggcgtcgg tctacggcgt cgtcgtggtc gtggtctggc
     2941 tctccacggt ggagtccggg ctgcatgcct cgatcgccgg catggccgcg gggctgctgg
     3001 tcgccgcctt cccgccgcgg cgggctgacg tggagcacgc ggcctcgttg ttccgggcct
     3061 tccgacagtc gccggcgccg ggcgtggggc ggacggccaa gcgtgggctg cagcgggcgg
     3121 tctcggtcaa cgaacggctg caggccaacc tgcacccctg ggtcagctat gtgatcgtgc
     3181 cggtcttcgc cctggccaac gccggggtgg acctgcgcgg cgggaccctc ggcgaggcgc
     3241 tgcgctcgcc gatcacgtgg ggcgtcgtac tcggccttgt cgtgggcaag ttcctcggca
     3301 tcagcgccgc ggcgctggcc accgtccggt tcacgatcgg ggccctgccc cacggcgtga
     3361 ctcggggcca ggttttcggt ggggcggcgc tgtcggggct cggctttacg gtgtcgctgc
     3421 tgatcgccag cctggccttc gactcggagg cgctgcgcga gcaggcaacg gtgggggtgc
     3481 tgctggccgc catgctctcc gccgcgaccg ggtggctggc cttccgcctc gaggggatcc
     3541 tcggcggcgc ggcagcggcg cgcccgctgg accttcaccc gcccgtggag atcggccacg
     3601 atcacatccg cgggccggtc gacgccccgc tgacgctggt ggagtacggc gacttcgagt
     3661 gcccgttctg tgcccgggcg accggggcgg cacgcgagct gcgcgcgatc ctcggcgacg
     3721 acctgcgcta tgtgttccga cacctgccgc tgagtgacgt gcaccccaac gctgagcagg
     3781 ccgcgctggc cgccgaggcc gccgccgcgc agggccggtt ctggcagatg cacgacctgc
     3841 tcttcgagaa ccaggaccag ctcgagaccg aggacctgat cggctacgcc gccgacctgg
     3901 acctcgacgt cgagcagttc gtgcgcgacc tcgacgacga cgagctggcc gcccgggtgc
     3961 gtgatgacgt ggccggcgcc gaggccagcg gcgcccgggg caccccgacc ttcttcatcg
     4021 gcggtcggct ctatgtcggc ccgcacgacg cggcgagcat cgcggcggcg ctgcggacgg
     4081 cgggcggcgg gaccgagccg gccgtcacaa acccaagtac gacgggctcg gtcgatggat
     4141 gagcaccggc acgccgacca cgcccaggcc gagcacgccc acggcgagca cgacaagcac
     4201 gcggggcact cggttgacat gttccgccgc cggttctggc ggagcctgct gctgaccatc
     4261 ccggtcgtgg ccaccagcga gatggtcatg gactggttcg gctaccggct ggacttccct
     4321 ggcctcgact gggtcgggcc gctgctcggg tcggtgatct tcttctgggg cggctggccg
     4381 tttctcgagg gcggccggcg cgaggtcatg gcgcgccagc ccgggatgat gctgctgatc
     4441 agcatggcga tcacggtcgc ctatgtcgcg tcgatggcga cctcgctggg ctggttcgag
     4501 ctcgacttct ggtgggagct ctcgctgctg gtgacggtca tgctgctggg ccactggcag
     4561 gagatgaagg ccctcggcca ggcgcaggac gccctcgccg cgctggcggc gctgctgcct
     4621 gacacggcca gcagggtcgg ccccggcgac gagatcgtca ccgtcgggct ggccgacctg
     4681 cgcgccggcg acgtggtgct ggtccgcccc ggcgaacggg tgcctgcgga cgcgaccgtg
     4741 ctcgagggct ccgccgagct cgacgagtcg atgatcaccg gggagtcccg cgccgtgacc
     4801 aagtccgccg gcgacaccgt gctggccggc accgtcacca ccgacaacgc gctgcgggct
     4861 cgggtcacgg ctgtcggcga gggcaccgcg ctggccggca tccagcgact cgtctccgac
     4921 gcccaggcgt cctcgtctcg ggctcaggac ctggccgacc gggccgccgc ggtgctcttc
     4981 tacgtcgcgg ccggcgtcgg cctgctcacc ttcgtggtct ggacgctgct cggggaggtc
     5041 cgcgaggcgg tcacccgcac cgtcaccgtg ctggtcatcg cctgcccgca cgcgctggga
     5101 ctcgcgatcc cgttggttat cgcgatcgcc accgagcgcg cggcccgggc cggtgtgctc
     5161 gtccgcaacc ggctggcgct ggagcggatg cgcaccgtcg acgtggtgct cttcgacaag
     5221 accggcacgc tgaccaaggg cgagcccgtg ctcacccagg cgagtggcac caacggcacg
     5281 ccggccgacg aggtgctcgc gctggccgcc gcggtcgaga ccgacagcga gcacccggtc
     5341 gcccgcgcga tcgtgcaggc cgccggcgag agggtgccgg ccggtgccca ccggtcgcgt
     5401 gccaccgact tccgggccag ccccgggcgc ggggtgcagg cccgggtcgg cgccagcacg
     5461 gtcgcggtcg gggggccggc catgctgacg gccgaaggac tggcggtccc cgccgagctg
     5521 gcggccgcga ctgtcccgtg ggtgcggcgg ggcgcctcgg tgctctacgt cgtacgcgat
     5581 ggtgccctgg tcggcgcgct cgcgctcgag gacgcggtgc gcgcagagtc cgcccaggcc
     5641 atcgacgcgc tgcacgcccg cggcctgagg gtcgccatga tcaccggaga cgcacgccgg
     5701 gtcgccgagt cggtggccgc gtcgctgcgc atcgacgagc acttcgccga ggtgctgccc
     5761 caggacaagg cggcctcggt ggccgcgctg caggcgcgcg ggcaccacgt ggcgatggtc
     5821 ggcgacgggg tcaacgacgc gcccgccctg gccggggccg aggtgggcat cgcgatcggc
     5881 gccggcaccg acgtggccat cgagtccgcc gacctggtgc tggccagcag cgacccgcgc
     5941 gcggtgctct cggcgatcga gctgtcccgg gccagctacc gcaagatgca gcagaacctg
     6001 gcctgggcca ccggctacaa cgtcgtcgcg atcccgctgg cggccggtgt gctcgagccg
     6061 gtcggcgtct ccatctcgcc ggccctgggc gccgtactca tgtcggtgtc cacgatcgtg
     6121 gtggcgctca acgcggcctt gctgcgccgt accgatcttg atccggcccg tctggccgcg
     6181 ccttagcggg ggcccgcagg tcacgcgacc gtttggccgc ggcctcggcg ccggcgtgct
     6241 tggctgagcc ggtggccgac atcaaggtgc gcaaccatgc cgaggagcag cgttacgagg
     6301 cctgggtgga cgggcggctc gcgggggtgg ccggctacca gacgacgcac gacctgatcg
     6361 tgttcacgca cacagacgtg gagcccgagt tcaagggcaa gggcatcgga tcggtcctgg
     6421 tccggcgcgc cctcgacgac gtccgggcgg agggcactca ccgggtgctg ccgttgtgcc
     6481 cgttcgtgaa gtcgtgggtc gccaaccacc gggagtacgc cgacctgctc tacagctcac
     6541 cgcgcagcac tgccactgac tgagcctccg cagcctcgtt ccatggcgcc gagcgccgtg
     6601 acggtcggac cgtgccggtt tgccctcagg gcagatgggg ataaaccggg catgtacgtc
     6661 gtggagatcc tcgaccacct cggcgatccc gcgcagttcg tcgtggcgcc gatcgtggcg
     6721 ctcgacctgg ccctgcgagc ccaacgcgcc ggactgcgct gggcggtcaa cgacgaggca
     6781 agcgcccgac cggtgtcgct ggaagacctc cggcaccagg ctcgccgact ggccgcaacg
     6841 gataccgccg tggccgaggc gctggaggag gccgaacgcg cgcatgagcg tggcctgcgc
     6901 gccaccctca actaggcggt tcccggcccc gacccgtgat catgcacgag tgtggtgctg
     6961 gtcttcggcg gccggtcacc cgccggcaaa tgactgatgt tggccattgc acccgccccc
     7021 caccgtcgtc agcctgggag ccgtcaggcc tacgcgcccc caccagcggg gaggtgagcg
     7081 tggcggtgac ccgcagggtc gtgcgacttg cggaggtcca tccctcccgc cagacggaca
     7141 cagcggtgcc cccggccaag gtggccacgc cgcggctgcc tgcgctgttc gtgccccggc
     7201 cccggctgca cggcttgctc accgaggcgg tggccagccg ggtcacgctg gtcagtgccg
     7261 gcccaggctc gggcaagacg ctgggcgtcg cctcctgggc ggcggacggt cgggcgcccg
     7321 gcccggtcgc ctggctggcc cttgacgaga gcgacaacga ccccgccacg ctctggcggg
     7381 agatcgtcgc ggcgttgcac gtcgcggggg cgccggcgtc gcgcgccggc gtgctgcacg
     7441 tctcgccgtt cggggcgccg gagatccgtc ggctgaccgc cgggttcacc tcgtcggccg
     7501 ccccggtcgt gctcgtggtc gacgacgtgg aagcgctcac gcgcgacgag gcccgcgcct
     7561 gtctcgacca gctcatcgcc gaggcgagcg cacagcttcg catcgtgctg atcacccgca
     7621 ccgatcccga cctgccgctg caccggctgc gcgtggtcgg tgacctcagc gagatccgcg
     7681 gcgacgagct ggccttcgac ggcgaggagg cgggccggct cttcgccgac cgggggttgt
     7741 ccctggatgc cggccagcgc gagagcctgc tggcgcgcac cgagggctgg gccaccgggc
     7801 tgcggctcgc gctgctcgcg ctctccgacg gtgacgtgga ccgggcgatc agcgacttct
     7861 ccggagacca gcgctccgtc gccgactacc tgatcggcga ggtgctgcgc cgccggccgc
     7921 cccaggtgca gcgcctgctg ctgcgcacga gcatcaccga gcgcatctgc ggcccgctcg
     7981 cagacctgct cgccgaccgc acgcacagtc agcgcgagct cgagcgcctg gcccaggctc
     8041 atccgctggt gcacagcctc gacgcccacc acacctggtt ccgctaccac cggctgctgc
     8101 gtgagctgct ccagcacgag ctgcagatcc aggagcccag cctggtgccc gagctgcacc
     8161 ggcgcgccgc agcctggtgg gaggcggccg gccaaccggt tgaggcgctc cggcacgcca
     8221 gcgcggcggg tgactggcgc cacgtccgcc gcctcctcgt cgacgtcgcc ctgccgatgc
     8281 tgctctcgcc ggaccgctcg acgctcgctg gtgcggtgcg ccctgcagcc gcgcaggcgg
     8341 ctgtgcgccc cagcctcgac acgttgatcc ccgccgcagc ctggcacctg ttcgagcgca
     8401 acatcccggt gatggacaag gacgccgacg aggccgaacg gcggctggga gagctcgacg
     8461 atgccgaggt gccgatggcg cgcgtggcga tcggactgtt ccggctgatg gccctgcgca
     8521 ccagcggaga cgccaacgag gtcgtgcggg tggccggcga gatgctgagc ctgctccggc
     8581 ttggccctgg ctcgcgtcct cctgcggcgc ggcactacga ggtggtcgcc gcggcccacc
     8641 tggcggccgg gctgctctgg ctgggcgagc acgcccaggc ccgccggcac ctcgtcactg
     8701 ccgcggccga ggcagacgct ctcgggcttg ggctgacccg gctctttgcg cactcgcacc
     8761 ttgcggtgct cgacgcgatg gaggggcggc tgcacgcggc cgagcagacg gcctcggcgg
     8821 tgctcgactc cgcggcccgt ggcgggtggg gcgacgagtc gcaggtgcag gcggctcgcc
     8881 tcgcgatcgc catggtccac cttgcccggg accggcccga cctggccggt cgccagctag
     8941 gagaaggtct cgacgggaca gggtccgcgg acggcgccgt acggctggcc atgaggattg
     9001 cgctggtgca cgtcgcgctg gcccaccgcg acccgtcgag tgcacgcctg gcctgttcgc
     9061 ggatccgcga tgagatcacg gtgagtgcgg cacctgactg ggtgctgggt tggttggcca
     9121 ccaccgaggc cgagaccgcg ctgctcacca acaacgccga gctcgccctc acgctgctca
     9181 agtcggtgcc gcacagccca gaggcgcagg tgctgcgcgt gcgggcccac ctcttgctgg
     9241 gcgaccgggt gggcgcctcc gatgcgctgg cgctgctgct ggcacggctg ccggtgagcc
     9301 tggcggtgca cgtgcaggtg tggctgctgg agggggtgct cgccaatgcc cgccacgacg
     9361 acgtctcggc catcgaggca ctgcgacgtg cgctcgcact ggccgagccg gaggagctgc
     9421 gacgtccgtt ccgggcgctg gggcccgacc tggccgcgac cgtggcgcgc tacctcgacg
     9481 tggtggaggg cctgggcgcc ttcgcgctcc gccacggcga cggcgggcaa gccgagggcg
     9541 ggccagagga ccggctcctg ctcgccgagc ggctgtccag ccgcgagctg gcggtgctga
     9601 gctacctgcc ctccatgctg accaatgagg agatcggcat ggcgctgttc gtctcggtca
     9661 acacggtcaa ggcccacctg aagtcgctct accgcaagct cggcgtcacc gggcggcgtg
     9721 aagcggtacg acgggcacgc gagctcgacc tgctgtgacc agacgtgcgc gccccgtcgt
     9781 cacccgcgct gggtgaggcg cgcgcggccc acgcatcgga cgcttcgggg gtccactgcg
     9841 aggaggagag ccatggccac attgacggcg tggaggttcg acacccccga tggtgccgag
     9901 gaggcagttg aaaccctgga gagtctcgcc aagcagcgtc tcatcacgct gcacgacgcg
     9961 gcgaccgtga catgggagcc cgacaagaag aagcccaaga cccgccagct caacaacctg
    10021 gtcggagccg gggcactcgg cggcgcgttc tgggggctgc tcttcggcct gatcttcttc
    10081 gtgccgctgc tgggcttggc catcggtgcg ggcatgggcg ccctgggcgg ttcgctcacc
    10141 gacgtcggca tcgacgacga cttcatccgc aaggcccgtg acgagatcac ccccggcacc
    10201 tcggccctgt tcgtgatgac ctccgacgcc gtggaggacc gggtgcgcga ggcgttctcg
    10261 gggcacaagg cgcacctgat gttcaccaac ctcagcgccg accaggagaa ggcgctgcgc
    10321 tcggtcttca gcgagtagtc agcagcgagc gagcacctag cggcgaggta gcgccgccat
    10381 ggccgagccc ggcaggcgtc gggctcggcc agcgccttgc ttgttgcgtg agcgtctcga
    10441 cggcgagacc ggcttctgag cgaaaccccg ggccgggtaa ggggttgcgg gctgtgcgtg
    10501 gggtccggct ctgtcagtgg tcgcgtctag gttgcggtca tggacggggt ggcgggcacg
    10561 ctgggtgggg cggtcgagca gatcaccgcc gggctcgcgc gcgtcgacga cgccgcattc
    10621 gtccgggcct ccgaccgtga gctgttggcc gcggtgccgg tgctggagtc gttgacggcg
    10681 cggctggagt gggtccgcgc gcacgtggtg ggcgaggtcg acgcgcgggg tctggctacc
    10741 gaggcgggag cctgctcgac accggcgtgg ctggccgggg tgagccgggc ccacccggga
    10801 tcgacctcgc gacttgtggg gttggcgcgg gcgttgcccc gct
//
LOCUS       DATIBB010000184         2310 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_380544, whole genome shotgun sequence.
ACCESSION   DATIBB010000184 DATIBB010000000
VERSION     DATIBB010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2310
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_380544"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..80
                     /gene="rrf"
                     /locus_tag="VLC50_07420"
     rRNA            <1..80
                     /gene="rrf"
                     /locus_tag="VLC50_07420"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            269..1738
                     /locus_tag="VLC50_07425"
     CDS             269..1738
                     /locus_tag="VLC50_07425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009080765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HSF27328.1"
                     /translation="MAELSICDLAAIEIAGRVRAREVSAREVVEAHIARVEAVDGVLN
                     ALVTRTFEQARAQAAAADDAAARGDALPLLHGLPVAHKDLAETRGVRTTYGSSIFAEH
                     VPTFDALHVARMRAAGAISLGKTNTPEFGAGSQTFNRVFGATRNPYDPTKTPGGSSGG
                     AAAALAVGEVALADGSDMGGSLRNPASFCNVVGLRPSPGRVPAWPSADGWSPLSVNGP
                     MARTVADVALLLTAISGPDHRVPLALDGSVAPEPVHEPADVTRLLERDLRGLRVAWSV
                     DLGGLPIDPAVRAALAPARAVLEGLGAEVVEAQPDLRGADQAFRAWRAFAYALSHQGL
                     LERHRDELKDTVVWNIEAGLSMSMSELLDATRARNQVMTAMAEFFGPRGADTGFDVLA
                     CPVSQVPPFDVDLEWVREIDGVPMTTYLDWMASACRISITGLPAMSVPAGFTDGGLPV
                     GLQLVGRPRDDWGLLQIGHAFETATGHGAVRPSLGALVA"
     gene            1735..>2310
                     /locus_tag="VLC50_07430"
     CDS             1735..>2310
                     /locus_tag="VLC50_07430"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: protein motif:HMM:NF015047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HSF27329.1"
                     /translation="MTDAAQARLADRLGLQVPVVAAPMAGGTSTVELVVAVCEAGGAA
                     FLPAGYLSIPTLQARIQEIRSKTSRPFGVNVMVPSQPLEDRSAVAAYGAALAETAGRL
                     GAGLGEPSWTDDDYAAKLDALTADPVPVVSFTFGCPRAEDVARLQRAGSLVLVTVTSP
                     VEATLARRVGADGLVAQGTEAGGHSACFHDDP"
BASE COUNT          292 a          839 c          854 g          325 t
ORIGIN      
        1 ttccgaaccc ggaagctaag cccttcagcg ccaatggtac tgcgcgggag accacgtggg
       61 agagtaggac gccgccggac aaccattgac gatggccacc cctgacaccc ctgtcagccg
      121 gtggccatcg ccatttcccc aacccggttg gcccgggatc ccaggccggc tgggcgatat
      181 tggcaagttg tgactgtggg tgcgccgcgc gggcggacta ctgtcaggcg gcgtggttgc
      241 gtgcgggacg tgtggaggat gacgaccggt ggctgagctg agcatctgcg acctcgcggc
      301 gatcgagatc gccggtcgcg tgcgcgcgcg tgaggtgtcc gcgcgtgagg tggtcgaggc
      361 acacatcgcc cgggtcgagg ccgtcgacgg cgtgcttaac gcgctcgtga cgcgcacgtt
      421 cgagcaggcc cgggcacagg ccgcagcggc ggacgatgcc gcggcccggg gggacgccct
      481 tccgctcctg cacggcctcc cggtcgccca caaggacctg gccgagaccc gcggggtgcg
      541 caccacctac ggctcctcca tcttcgccga gcacgtgccc acgtttgacg cgctgcacgt
      601 ggcccggatg cgggcggccg gggcgatctc gctcggcaag accaacacgc ctgagttcgg
      661 ggccggctcg cagacgttca accgggtctt cggggccacc cgtaacccct acgacccgac
      721 gaagacaccc ggcggcagca gcgggggtgc ggccgcggcg ctggccgtcg gcgaggtggc
      781 cctggccgac ggcagcgaca tgggtgggtc gctgcgcaac ccggccagct tctgcaacgt
      841 ggtcgggctc cggccctcgc cgggtcgggt gccggcctgg cccagcgccg acggctggtc
      901 gcccctgagc gtcaacggcc cgatggcccg caccgtcgcc gacgtggcgc tgctgctcac
      961 cgccatcagc ggccccgacc atcgggtccc gctcgcgctg gacggctcgg tggccccgga
     1021 gccggtgcac gagcccgccg acgtgacccg cctgctcgag cgtgacctgc gcgggctgcg
     1081 cgtggcctgg agcgtcgacc tgggcgggct gccgatcgac ccggcggtgc gcgccgccct
     1141 ggccccggcc cgggcggtgc tcgaggggct cggcgccgag gtcgtcgagg cccagcccga
     1201 cctgcgcggc gccgaccagg cgttccgcgc ctggcgggcc ttcgcctatg cgcttagcca
     1261 ccagggtctg ctggagcgcc accgagacga gctcaaggac acggtggtct ggaacatcga
     1321 ggcgggtctg tcgatgtcga tgagcgagct gttggacgcc acccgggccc gcaaccaggt
     1381 catgacggcg atggccgagt tcttcgggcc ccggggcgcc gacaccggct tcgacgtact
     1441 tgcctgcccg gtctcacagg tgccgccgtt tgacgtcgac ctcgagtggg tgcgcgagat
     1501 cgacggcgtg ccgatgacga cctatctcga ctggatggcc tccgcctgcc ggatctcgat
     1561 caccgggctg ccggccatgt cggtgccggc cgggttcacc gacgggggtc tgccggtggg
     1621 gctgcagctg gtcggccggc cgcgcgatga ctggggtctg ctgcagatcg ggcacgcgtt
     1681 cgagaccgcc accggtcatg gcgcggtgcg cccctcgctg ggcgcgctcg tcgcatgacc
     1741 gacgcagcgc aggcgcgatt ggccgaccgg ctagggcttc aggtccccgt tgtcgccgcc
     1801 ccgatggcgg gcggcacctc gaccgtcgag ctcgtggtgg cggtctgcga ggccggcgga
     1861 gcggcgttcc tgcccgccgg ctacctgagc atcccgaccc tgcaggcgcg catccaggag
     1921 atccggtcca agaccagccg gcccttcggc gtcaacgtga tggtgccaag ccagccgttg
     1981 gaggaccgct cggccgtcgc ggcctacggc gccgccctgg ccgagaccgc cggccgcctc
     2041 ggagccgggc tgggcgagcc cagctggacc gacgacgact acgccgccaa gctcgacgcg
     2101 ctgaccgcgg accccgtgcc tgttgtcagc ttcacgttcg gctgcccacg ggccgaggac
     2161 gtcgcgcggc tgcagcgggc cggcagcctc gtcctggtca ccgtcacgtc cccggtcgag
     2221 gcgaccctgg cccgacgcgt cggcgccgac gggctggtgg cgcagggcac cgaggccggc
     2281 gggcactcgg cgtgcttcca cgacgacccg
//
LOCUS       DATIBB010000185         2190 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_1168778, whole genome shotgun sequence.
ACCESSION   DATIBB010000185 DATIBB010000000
VERSION     DATIBB010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2190
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1168778"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..428)
                     /locus_tag="VLC50_07435"
     CDS             complement(<1..428)
                     /locus_tag="VLC50_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016571404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HSF27330.1"
                     /translation="MDAREIAEARRRWQARFDAARTRDADFTTLSGLAVEPVYGPPED
                     SADQRMARVGWPGEFPFTRGLYPTGYRGRTWTIRQFAGFGNAAQTNERYRMILGAGGG
                     GLSVAFDMPTLMGRDSDDPRSLGEVGHCGVAIDSAADMDVL"
     gene            complement(549..857)
                     /locus_tag="VLC50_07440"
     CDS             complement(549..857)
                     /locus_tag="VLC50_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005272316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH1187 family thiamine-binding protein"
                     /protein_id="HSF27331.1"
                     /translation="MLVAFSVAPAGVGESVSAAVADAVRIVRESGLPHRTDSMFTTIE
                     GEWDEVMDVVRRATEAVGRHSGRVSLVLKADIRPGHTGEMTGKLERLEAAIEAAGSTD
                     "
     gene            complement(899..2023)
                     /locus_tag="VLC50_07445"
     CDS             complement(899..2023)
                     /locus_tag="VLC50_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015104635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSF27332.1"
                     /translation="MTTIGPARHDQAPAPGRLAQRGEASSLAQLRTLSLAMLRGFFRD
                     KAALFFTFFFPLMFLVVLGLIFGGSAETKPTAIGVVGSGPVISALPPQVVKPQPFDSL
                     DQALEQVRAGDLPAVLVEDGDDLTLRFSAADPVGAATIRGIIASVVNQANLVATGQPP
                     RFTLTAEQVESADLQPIQFITPGILSWAVATSAAFGAALVLVSWRRKQILRRVRLSPA
                     PVWTVLGARVGVSLVVALVQAAVFIGVALTPPFGLRLSGDWWLAIPLLVAGTLAFLAV
                     GLFVGAVAKTEEAASAIANFVVLPMAFLSGTFFDISQAPGWLQTVSLALPLRHLNDGM
                     LDVLVRGRGVEAIVWPTVILLGFAAAVVAIASRLFRWEDS"
     gene            complement(2020..>2190)
                     /locus_tag="VLC50_07450"
     CDS             complement(2020..>2190)
                     /locus_tag="VLC50_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005447771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSF27333.1"
                     /translation="GADDVHGDDGEVVVSTRSPARLVAALAERDCLDGVSVRTATLED
                     VFLHLTGREYRA"
BASE COUNT          339 a          777 c          817 g          257 t
ORIGIN      
        1 agcacgtcca tgtcggccgc cgagtcgatg gcgaccccgc agtggcccac ctcgcccagc
       61 gagcgcgggt cgtcggagtc gcgacccatc agcgtcggca tgtcgaacgc caccgacagg
      121 ccaccaccgc cggcgccgag gatcatccgg tagcgctcgt tggtctgggc agcgttgccg
      181 aagccggcga actggcggat cgtccaggtg cgcccgcggt agccggtcgg gtagagcccg
      241 cgggtgaacg ggaactcgcc gggccagccg acccgcgcca tccgctggtc ggcgctgtcc
      301 tcgggcgggc catagaccgg ctcgaccgcc agcccggaca gcgtggtgaa gtcggcgtcg
      361 cgggtgcggg cagcgtcgaa gcgggcctgc cagcggcgac gggcctcggc gatctcgcgt
      421 gcgtccacgg gggtctccca gtgcgggcag gcggcgcgcc gtgcggggtg tgacggcgga
      481 ccgcctcgac gagtacggcg tgtcacagcc agggtagccg tcgacgcagg cgacccgccc
      541 ggcaccgctc agtcggtgct gcccgccgcc tcgatcgcgg cctcgagccg ctcgagcttg
      601 ccggtcatct caccggtgtg gccgggccga atgtcggcct tgagcacgag cgagacgcgg
      661 ccggagtgtc ggccgaccgc ctcggtggcc cggcgaacga cgtccatcac ctcgtcccac
      721 tcgccctcga tggtggtgaa catcgagtcg gtgcggtgcg gcaggcccga ctcacggacg
      781 atgcgtacgg cgtcggccac ggccgcagag accgactcgc cgacgccggc gggggcgaca
      841 gagaaggcaa ccagcatggg ccgaccctat gacccgcgcc ccgatccggc gccgcgggtc
      901 aggagtcctc ccagcggaag agccggctcg cgatcgcgac gaccgccgcg gcgaagccga
      961 gcaggatgac cgtgggccag acgatcgcct cgacgcccct gccacgcacc agcacgtcga
     1021 gcatgccgtc gttgaggtgg cgcagcggca gggccaggct caccgtctgc agccacccgg
     1081 gcgcctggga gatgtcgaag aacgtgcccg acaggaatgc catcggcagc acgacgaagt
     1141 tggcgatcgc gctggccgcc tcctcggtct tggccacggc tccgacgaac aggccgaccg
     1201 cgaggaaggc cagcgtgccc gcgacgagca gcgggatggc cagccaccag tcccccgaca
     1261 gccgcagccc gaacggcggc gtcagcgcga cgccgatgaa cacggccgcc tgcacgagcg
     1321 cgacgacgag gctgacgccg acccgtgccc cgagcacggt ccacaccggc gcgggcgaga
     1381 gccggactcg gcgcaggatc tgcttgcgcc gccaggagac cagcaccagc gcggcaccga
     1441 aggcggccga cgtggccacc gcccagctga ggatgcctgg ggtgatgaac tggatcggtt
     1501 gcaggtcggc cgactccact tgctcggccg tgagggtgaa ccggggtggc tggccggtcg
     1561 ccacgaggtt ggcctggttg acgaccgagg cgatgatccc tcggatcgtg gcggcgccca
     1621 ccgggtcggc cgccgagaac cgcagcgtga ggtcgtcgcc gtcctcgacc agcaccgccg
     1681 gcaggtcgcc ggctcggacc tgctcgaggg cctggtcgag agagtcgaac ggctggggct
     1741 tgaccacctg cggcgggagc gcagagatca ctgggccgga gccgaccacc ccgatcgccg
     1801 tcggcttggt ctcggcggat ccgccgaaga tcagcccgag tacgacgagg aacatgagcg
     1861 ggaagaagaa cgtgaagaac agcgccgcct tgtcgcggaa gaagccgcgc agcatcgcca
     1921 ggctcagggt gcgcagctgc gcgagcgagg aggcctcgcc gcgctgcgcg agccggcccg
     1981 gggccggcgc ctggtcgtgg cgggccgggc cgatggtggt catgcccggt actcgcgtcc
     2041 ggtgaggtgc aagaagacgt cctcgagcgt ggcggtgcgc acgctcaccc cgtcgaggca
     2101 gtcccgctcg gccagggcgg ccactagccg ggccggcgag cgggtggaga ccaccacctc
     2161 cccgtcgtca ccgtggacgt cgtcggctcc
//
LOCUS       DATIBB010000186         3659 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_908080, whole genome shotgun sequence.
ACCESSION   DATIBB010000186 DATIBB010000000
VERSION     DATIBB010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3659)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3659)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3659
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_908080"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            184..696
                     /gene="ruvX"
                     /locus_tag="VLC50_07455"
     CDS             184..696
                     /gene="ruvX"
                     /locus_tag="VLC50_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020547122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HSF27334.1"
                     /translation="MRRGVRVAVDVGSVRVGVAVSDPDGLVATPLRTVARGKGRDAGA
                     DLAEIAAVVAERDAFEVIVGLPIRLQGDEGPAAAAARDYATALAAQVAPVPVRLVDER
                     LSTAAATRGLRDTGVRGRKSRTVIDQAAAVLILQGALDAERASGRPCGEHVPAPPAAG
                     GPDEAEPTAR"
     gene            693..1805
                     /gene="mltG"
                     /locus_tag="VLC50_07460"
     CDS             693..1805
                     /gene="mltG"
                     /locus_tag="VLC50_07460"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HSF27335.1"
                     /translation="MSHLGLPMTDEEHDGPRSRSHRAHKRRRRGWFAVLIACLALALV
                     VAGVWFAIGGLGERVRSSLAGPADYPGPGTGSVQVQVQPGESAGAIGRTLKAADVVAS
                     VESFTTAARADERSKGIQPGYYVLKTKMSGQGALALMLDPASRLVTRVTVPEGLRLDE
                     TVKLLAAKAGFTVADLEKALADPAALGLPAEADGQAEGYLYPATYDVEPDDTPASVLK
                     AMVAQYAKVAAAVGVEDGPVSAHEVVTIASIVEAEARHAEDYGKVARVIYNRLAKKMP
                     LQMDSTVNYALKADKEIVTFDDLEVESDYNTYKHPGLPPGPIGSPGQAALEAALAPAD
                     GDWLYFVTVDPASGETKFTASYDEFLRFKQELKANS"
     gene            1802..2659
                     /locus_tag="VLC50_07465"
     CDS             1802..2659
                     /locus_tag="VLC50_07465"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020138092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HSF27336.1"
                     /translation="MTAARRAAVLGSPIAHSLSPVLHRAAYDALGLRDWTYDAVEITP
                     ATLGGFLDGLGPEWAGLSLTMPLKQAVIPLLDAVSAGAREVGAVNTVVLRDGRREGTN
                     TDVPGFVAALRERGVTACAAPVVLGAGATAGSALAALAALGTRTATVVARRPSAAGPV
                     CEVGARVGLDVEVVEWAVPGPALDAVRSADLVVATTPAGAADGLAAALAGGGAAPGGV
                     LFDVVYAPWPTRLGAVWSSYGLMVLSGLDLLVHQAALQVPAFTGLDLDPAALVVPMRA
                     AGLQALAAR"
     gene            2887..>3659
                     /locus_tag="VLC50_07470"
     CDS             2887..>3659
                     /locus_tag="VLC50_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HSF27337.1"
                     /translation="MKQLADILLDDGLVTDEQLNAAFEEQERAGRSLGRVLVDTGVLS
                     EAQLVAALAAQIGMRFVDLTDTTIDGSVIGAVPGNVARRHCVLPIGVEDGKIVVAMAD
                     PANVFAIDDIRSITGLEVRPVVATRGDVIAGIDRYYRADSDLDELTNAMDMGEELEDL
                     SKIKEVVDDAPIVKYVNLLITQGIQDGASDIHLEPGERDLRVRFRIDGVLHDVMRSPK
                     AIQSGVISRLKIMADINIAERRIPQDGRLSVSAHGKKVDL"
BASE COUNT          494 a         1304 c         1377 g          484 t
ORIGIN      
        1 ccgcgacgac gctcggcggg ggcggcggcg gcaaggacga tgtggcccag gggggcggca
       61 ccaacccggg tgccatcgac gaggcgctgg cccagctcga ggcggccctc ggcgcccggg
      121 ccagcaacgg ttgacggtga cggccgcgac gggcgcgggg cggggggcct cgtgacgggc
      181 gccgtgcgcc ggggggtgcg ggtggccgtc gacgtgggca gtgtgcgcgt cggggtggcg
      241 gtcagcgacc ccgacgggtt ggtggccact ccgctgcgca ccgtcgcgcg cggcaagggc
      301 cgagacgccg gcgccgacct cgccgagatc gccgcggtgg tggccgagcg cgacgcgttc
      361 gaggtgatcg tggggctgcc gatccggctg cagggtgacg agggaccggc ggccgcggcg
      421 gcccgcgact acgccaccgc gctcgctgcc caggtggccc cggtgccggt gcgcctggtc
      481 gacgagcggc tgagcaccgc cgcggccact cgcgggttgc gcgacaccgg ggtgcgcggg
      541 cgcaagagtc ggaccgtgat cgaccaggcc gccgccgtgc tcatcctgca gggggcgctg
      601 gacgccgagc gtgcctctgg gcgaccctgc ggtgagcacg tgccagcccc gcccgccgcc
      661 ggcgggcccg acgaagcgga gccgaccgcg cgatgagcca cctcggcctg cccatgaccg
      721 acgaggagca cgacgggcct cgcagtcgca gccatcgcgc ccacaagcga cgcaggcgcg
      781 gctggttcgc cgtactcatc gcctgtctgg ccctggccct ggtcgttgcc ggggtgtggt
      841 tcgccatcgg cgggctgggc gagcgggtgc gctcgtcgct ggccggcccg gccgactacc
      901 cgggcccggg caccggctcg gtgcaggtgc aggtgcagcc gggcgagtcg gccggcgcga
      961 tcgggcgcac gctcaaggcg gccgacgtgg tggccagcgt cgagtcgttc accaccgccg
     1021 cgcgcgccga cgagcggtcc aagggcatcc agcccggcta ctacgtgctc aagaccaaga
     1081 tgagcggcca gggtgcgctg gccctgatgc tcgacccggc cagccggctg gtcacccggg
     1141 tcaccgtgcc cgaggggctg cgcctcgacg agacggtcaa gctgctcgcc gccaaggccg
     1201 gcttcaccgt ggccgacctc gagaaggcgc tggccgatcc cgcggcgctg ggtctgcccg
     1261 ccgaggccga cgggcaggcc gagggctacc tctacccggc gacctacgac gtggagcccg
     1321 acgacacccc cgcctcggtg ctcaaggcga tggtggcgca gtacgccaag gtggccgccg
     1381 ctgtcggggt cgaggacggc ccggtgtccg cgcacgaggt ggtgacgatc gcgagcatcg
     1441 tcgaggccga ggcccgtcac gccgaggact acggcaaggt cgcccgggtg atctacaacc
     1501 ggctggccaa gaagatgccg ctgcagatgg actccacggt caactacgcg ctcaaggccg
     1561 acaaggagat cgtcaccttc gacgacctcg aggtggagtc cgactacaac acctacaagc
     1621 acccggggtt gccgccgggc ccgatcggct cgcccggcca ggccgcgctc gaggcggcgc
     1681 tggccccggc cgacggcgac tggctctact tcgtcaccgt cgacccggcg agcggggaga
     1741 ccaagttcac cgccagctac gacgagttcc tgcggttcaa gcaggagctc aaggccaact
     1801 cgtgacggct gctcggcggg cggcggtgct gggctcgccg atcgcgcact cgctctcgcc
     1861 ggtgctgcac cgggcggcgt acgacgcgct cgggcttcgc gactggacgt acgacgcggt
     1921 cgagatcacc cctgccacgc tgggcggctt cctcgacggg ctgggcccgg agtgggccgg
     1981 gctgtcgctg accatgccgc tcaagcaggc ggtcatcccg ctgctcgacg ctgtctccgc
     2041 cggtgcacgc gaggtcggcg cggtcaacac cgtggtgctg cgcgacggcc ggcgtgaggg
     2101 caccaacacc gacgtgccgg ggttcgtggc cgcgctgcgc gagcgcggcg tgaccgcatg
     2161 cgcggcgccg gtggtgctcg gtgccggcgc caccgccggc tcggccctgg cggcgctggc
     2221 ggccctaggg acacgtacgg cgaccgtggt ggcccgccgg ccgtccgcgg ctggcccggt
     2281 ctgcgaggtc ggcgcccggg ttgggctcga cgtcgaggtc gtcgagtggg cggtgcccgg
     2341 cccggcgctc gacgcggtga ggtccgccga cctggtggtg gctaccaccc ccgccggggc
     2401 cgccgatggc ctggccgccg ccctggccgg gggaggggcg gcgccgggcg gagtgctctt
     2461 cgacgtggtc tacgccccct ggccgacccg gctgggcgcg gtctggtcgt cgtacggcct
     2521 gatggtgctc agcgggcttg acctgctggt gcaccaggcc gcgctgcagg tgccggcctt
     2581 caccgggctc gacctcgacc cagccgcgct ggtcgtcccg atgcgcgccg ctggccttca
     2641 ggcgctggcc gctcgataac ccagccgacc tcgccagggc cggacacaaa ggacggacca
     2701 atcgcgcggg acggccgttt gacgtgcttc cggggtcgtt gttccgtcct ttgtgctcgc
     2761 cacctgactc ggagcggctc gccatgcaca actgcatgat caccgacggg gggagtggcg
     2821 atgccgccgg cctgcaggct ctgaccagcg gttaagcccc gcggctgccg tgccgatagg
     2881 gggcatgtga agcagctcgc cgacatcctg cttgacgacg gcctggtgac cgacgagcag
     2941 ctcaacgctg ccttcgagga gcaggaacgc gccgggcgca gcctgggccg ggtcctcgtc
     3001 gacaccggcg tgctcagcga ggcccagctg gtggccgcgc tggccgccca gatcggcatg
     3061 cgcttcgtcg acctgaccga caccacgatc gacgggtcgg tgatcggcgc cgtacccggc
     3121 aacgtggccc gccggcactg cgtgctgccg atcggcgtcg aggacggcaa gatcgtcgtg
     3181 gcgatggccg acccggccaa cgtgttcgcg atcgacgaca tccgctcgat caccgggctc
     3241 gaggtccggc cggtcgtcgc gacccgcggc gacgtgatcg cgggcatcga ccgctactac
     3301 cgggccgact ccgacctcga cgagctcacc aacgccatgg acatgggcga ggagctcgag
     3361 gacctctcca agatcaagga ggtggtcgac gacgcgccga tcgtcaaata cgtcaacctc
     3421 ctgatcaccc aggggatcca ggacggcgcc tccgacatcc acctcgagcc gggggagcgt
     3481 gacctgcggg tgcgtttccg catcgacggt gtgctgcacg acgtcatgcg ctcgcccaag
     3541 gcgatccagt cgggtgtgat cagccggctc aagatcatgg ccgacatcaa catcgccgaa
     3601 cggcggatcc cgcaggacgg ccggctctcg gtcagcgcgc acggcaagaa ggtcgacct
//
LOCUS       DATIBB010000187         4341 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_119491, whole genome shotgun sequence.
ACCESSION   DATIBB010000187 DATIBB010000000
VERSION     DATIBB010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 4341)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4341)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4341
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119491"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..758)
                     /locus_tag="VLC50_07475"
     CDS             complement(<1..758)
                     /locus_tag="VLC50_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013422282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HSF27338.1"
                     /translation="MTIGIVAFIVALIISVMLHEAGHFLFARRFGMKATEFFVGFGPK
                     LWSRQRGETEYGVKGILAGGYVKIIGMTPLEQVDEADKPRAFYLQPWPKRLVVLVAGS
                     AVHVVIAVLLVYFVLVGLGTPRYDPSLNVAEVSRCVPTSATDACAPGDPASPAAAAGL
                     KSGDTVVSFDGAAVTSWDQLSAAIREHGAGPAPIVVERDGARVTLTPDLVERERPTSP
                     GATSTAQVGVLGVSPEQTATMVRSGPIEAVGTTG"
     gene            complement(761..2008)
                     /gene="dxr"
                     /locus_tag="VLC50_07480"
     CDS             complement(761..2008)
                     /gene="dxr"
                     /locus_tag="VLC50_07480"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018655232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HSF27339.1"
                     /translation="MPDNGSVSAQRDVVILGSTGSVGTQALDICRRNPDRFRVVGLAA
                     GGADVALLAAQALELAVPVVAVARATVAEDLTLALNAEAARRGFATGDFALPRVLAGP
                     EAASEVAALPADVVLNGITGAVGLAPTLAALAAGRTLALANKESLIVGGPLVKALAAP
                     GQIVPVDSEHSAVAQCLRGGTADEVRRLVLTASGGPFRGRSRTELVDVTPEQALAHPN
                     FAMGPVLTTNSATMMNKGLEVIEAHLLFDLPYDRIDVVVHPQQMIHSMVEFTDGSTLA
                     QASPPSMLIPIALGLAWPDRVPEAAPGCDWTTADTWEFFPLDEAAFPAVPLARAAGQA
                     GGTAPAVLNAANEECVAAFLAGRLPFLAIIDTVEAVLAEHDVAGADGTLTLPDVMAAQ
                     HWARTRARELTGLDAGDRVGEPL"
     gene            complement(2019..3521)
                     /locus_tag="VLC50_07485"
     CDS             complement(2019..3521)
                     /locus_tag="VLC50_07485"
                     /inference="COORDINATES: protein motif:HMM:NF037653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator
                     ligand-binding domain-containing protein"
                     /protein_id="HSF27340.1"
                     /translation="MSVTIAQVIGESGLGLRLVVAPRERLDAQVRWVAVTELADPRPF
                     LAGGELVLTTGLNQGSALDQARFVERVAERGAAGLGFAVGLTHPKVPEAVVAAARRLD
                     LPLLEVPYAIPFIAIDRWVADRVLAGHYGRFRALLQMYDELSRALLSGQGLSTVVDVL
                     RGFVGGDVGVVDHIGSVLASAPATTGWPVEQIATVRADPTAVVAGTPLAALPVEVDKT
                     VVAHLCLRHPVDSLDVLPYAVSLVGLELARRQAVLTGRRELLGQVVEDLVKGVVAPAE
                     AERRLAAAGLDPAASHRVVLGEVDGDEAARDRLRSLPWAVQPPVVTAQVGAQLAVLAG
                     ADQPAHELAHLLHGQLRALAPSARVGIGGAYPGVAGLRWSFFEAREALARGPGINDRE
                     PLSLSGLILGSQDLPMRELGEDVLRPLLDFDATHAGELVATLRAYLEVDGSVSAVAER
                     LFVHRNTVRYRLAQVERLTGRSLTSTQDRVQLWLALQAVALGAPDRTTAP"
     gene            3655..>4341
                     /locus_tag="VLC50_07490"
     CDS             3655..>4341
                     /locus_tag="VLC50_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020517455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HSF27341.1"
                     /translation="MVVEQIPARVAGRAVTGDRWLHVINPYDGTEVGRVPACGPDDVD
                     AACTGAAAALDRDDFPQHRRAEVLDRAAVLLGERSEEFARTVALEAAKPLRDARGEVA
                     RGVDTLRLSAAAARTLAGQMLPLEGSAGGVGRLGFTLRVPVGVVAAITPFNFPLNLVA
                     HKLGPAIAAGAPVVLKPAEQTPITAIRLVDLLVEAGLPDDWVSVLTGTGAEVGAPLVA
                     HPLVRLVSFTG"
BASE COUNT          614 a         1613 c         1577 g          537 t
ORIGIN      
        1 ctgccggtgg tgccgactgc ctcgatcggg ccggagcgga ccatggtggc ggtctgctcg
       61 gggctcacgc cgagcacgcc gacctgcgcg gtactcgtcg cgcccggcga ggtgggccgc
      121 tcgcgctcca ccaggtcggg ggtaagcgtg acccgggccc cgtcgcgctc gacgacgatc
      181 ggggcgggac cggcgccgtg ctcgcggatc gccgcggaga gctgatccca cgacgtgacg
      241 gcggccccgt cgaaggagac gacggtgtca cctgacttga ggccggcagc cgcggccggg
      301 gaggccgggt cgccgggcgc gcaggcgtcc gtggccgacg tgggcacgca gcgggacacc
      361 tcggccacgt tcaggctcgg gtcgtagcgc ggggtgccca gcccgacgag cacgaagtag
      421 acgaggagta cggcgatgac cacgtgcacc gccgagccgg cgaccagcac gaccagccgc
      481 ttgggccacg gctgcaggta gaacgcccgc ggcttgtcgg cctcgtcgac ctgctcgagc
      541 ggggtcatcc cgatgatctt gacgtagccg ccggccagga tgcccttgac gccgtactcg
      601 gtctcaccgc gctgacgcga ccacagcttg ggcccgaagc cgacgaagaa ctcggtggcc
      661 ttcatgccga accggcgggc aaagaggaag tggccggcct cgtgcagcat caccgagatg
      721 atcagcgcca caatgaaggc gacgatgccg atcgtcatgc tcatagcggc tcacccaccc
      781 ggtcaccggc atccaggccg gtcagctcgc gcgcccgggt ccgcgcccag tgctgggccg
      841 ccatcacgtc gggcagggtc agcgtcccgt cggcgccggc cacgtcgtgc tcggccagca
      901 ccgcctcaac ggtgtcgatg atggccagga acggcagccg gccggccagg aaggcggcca
      961 cgcactcctc gttggcggcg ttgagcacgg ccggcgcagt gcccccggcc tgcccggcgg
     1021 cccgagcaag gggtacggcg gggaacgcgg cttcatccag cgggaagaac tcccaggtgt
     1081 ccgccgtcgt ccagtcgcag cccggcgccg cctcaggcac ccggtcgggc cacgccagcc
     1141 cgagcgcgat ggggatcagc atgctcggcg gcgaggcctg ggcaagggtg gacccgtcgg
     1201 tgaactcgac catcgagtgg atcatctgct gggggtggac cacgacgtcg atgcggtcgt
     1261 agggcaggtc gaagagcaga tgggcctcga tcacctcgag ccccttgttc atcatcgtgg
     1321 ccgagttggt cgtcaggacc gggcccatcg cgaagttggg gtgggccagc gcctgctcgg
     1381 gcgtcacgtc gaccagctcg gtgcgcgagc ggccgcggaa cgggccaccg ctggcggtga
     1441 ggaccagccg gcgcacctca tcagccgtcc cgccgcgcag gcactgggcc actgctgagt
     1501 gctcggagtc gaccggcacg atctgcccgg gcgcggcgag cgccttgacc agcgggccgc
     1561 ccacgatgag cgactccttg tttgccagcg cgagcgtgcg cccggccgcc agcgcggcga
     1621 gcgtgggggc gagcccgacc gcgccggtga tcccgttgag caccacatcg gccggcagcg
     1681 cggcgacctc gctggcggcc tcggggccgg ccagcacccg cggcagcgcg aagtcgccgg
     1741 tggcgaagcc gcgccgggcc gcctcggcgt tgagcgccag ggtgaggtcc tccgccacgg
     1801 tggcccgggc caccgcgacc accggcaccg cgagctccag cgcctgggcg gccaggaggg
     1861 ccacgtcggc cccgcccgcg gccagcccca cgacccggaa ccggtcggga ttgcggcggc
     1921 agatgtcgag ggcctgggtg ccgaccgaac cggtcgagcc caaaatgacg acgtcccgtt
     1981 gtgcgctcac tgacccattg tccggcatcc tcgcccgatc atggtgcagt ggtgcgatcg
     2041 ggcgcgccca gcgccaccgc ctgcagcgcc agccacagct gcacgcggtc ttgcgtcgag
     2101 gtcagcgagc gaccggtgag ccgctccacc tgggccagtc ggtagcggac cgtgttgcgg
     2161 tgcacgaaca gccgctcggc taccgcgctc accgacccgt cgacctccag gtaggcccgc
     2221 agcgtggcga cgagctcgcc ggcgtgggtg gcgtcgaagt cgagcagcgg gcgcagcacg
     2281 tcctcgccga gctcgcgcat cggcaggtct tggctgccca ggatcagccc ggacaggctc
     2341 agcggctctc tgtcgttgat gccgggtccg cgggccagcg cctcacgggc ctcgaagaag
     2401 ctccagcgca gccccgccac cccggggtag gccccgccga tgccgacccg ggccgagggg
     2461 gcgagcgcgc gcagctgccc gtgcagcagg tgggccagct cgtgggcggg ctggtcggcg
     2521 ccagcaagta cggcgagctg ggcccccacc tgtgccgtga ccaccggggg ctgcaccgcc
     2581 cagggcagcg accgcagccg gtcgcgggcc gcctcgtcgc cgtcgacctc gcccagcacg
     2641 acccggtggc tggccgccgg gtccagcccg gcagccgcca gccggcgctc ggcctcggcc
     2701 ggtgccacga cccccttcac gaggtcctcg accacctgcc cgagcagctc gcgccggccg
     2761 gtgagcaccg cctgacgccg tgccagctcg agcccgacca ggctcaccgc gtagggcagc
     2821 acgtcgagcg agtcgaccgg gtggcgcagg cacaggtgcg cgaccacggt cttgtccacc
     2881 tcgaccggca gcgcggccag cggggtgccc gccaccaccg cagtggggtc ggcccgcacc
     2941 gtggcgatct gctccaccgg ccagccggtg gtggccggtg cactggccag cacgctcccg
     3001 atgtggtcga ccacgcccac gtcaccgccg acgaacccgc gcagcacgtc gacgacagtg
     3061 ctgagcccct gacccgacag gagcgcccgg gagagctcgt cgtacatctg cagcagtgcc
     3121 cggaaccggc cgtagtgccc cgcgaggacc cggtcggcca cccagcggtc gatcgcgatg
     3181 aacgggatgg cgtagggcac ctcgagcagc ggcaggtcga gccggcgcgc ggccgcgacc
     3241 acggcctcgg gcaccttggg atgggtcagc ccgaccgcga atcccagccc ggccgcgccg
     3301 cgctcggcga cacgctcgac gaagcgggcc tggtcgagcg cgctgccttg gttgagcccg
     3361 gtcgtgagaa ccagctcacc gccggccagg aacgggcgcg ggtcggccag ctcggtgacc
     3421 gcgacccagc gcacctgcgc gtcgagcctc tctcgagggg ccaccaccag ccggagtccc
     3481 aacccggact ccccgatgac ctgcgcaatc gtgaccgaca cgccatcact gtactgatgc
     3541 acaacaaaga agatcagttt tgttctgaca tccgtggcgt gcatcgcagg gcggtcctag
     3601 cgtcggatcg gtcagcgcac ggaacaggca cgaagcgggg ggcgggccgc cacgatggtc
     3661 gtcgagcaga ttccggctcg ggtcgccggg cgtgcggtga ccggggaccg gtggctgcac
     3721 gtcatcaacc cctacgacgg caccgaggtg ggccgagtcc cggcctgcgg gcccgacgac
     3781 gtcgacgccg cctgcactgg cgcggccgcc gcactcgatc gcgacgactt cccgcagcac
     3841 cggcgggccg aggtgctcga ccgtgccgcc gtcctgctcg gcgaacgcag cgaggagttc
     3901 gcccgcactg tggcgctgga ggccgccaag ccgcttcgcg acgcccgcgg cgaggtcgcc
     3961 cgcggcgtcg acacgttgcg gctctcggcg gccgccgccc gcacgctggc cgggcagatg
     4021 ctgccgctgg agggctcggc cggcggggtc gggcgcctcg ggttcacgct ccgggtgccg
     4081 gtcggggtcg tcgccgcgat caccccgttc aacttcccgc tcaacctggt cgcgcacaag
     4141 ctcggccccg ccatcgccgc cggcgccccg gttgtgctca agcccgccga gcagacaccg
     4201 atcacggcga tccgcttggt cgacctgctc gtcgaggcag gcctgcccga cgactgggtc
     4261 tcggtgctca ccggcaccgg cgccgaggtc ggagcaccgc tggtggctca cccgctggtg
     4321 cggctcgtct cgttcaccgg c
//
LOCUS       DATIBB010000188         2265 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_1438778, whole genome shotgun sequence.
ACCESSION   DATIBB010000188 DATIBB010000000
VERSION     DATIBB010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2265)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2265
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1438778"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1019)
                     /locus_tag="VLC50_07495"
     CDS             complement(<1..1019)
                     /locus_tag="VLC50_07495"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020573355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HSF27342.1"
                     /translation="MPARTLVVTNDFPPRTGGIESFVLAMTTRMPADSVVVLTARQPG
                     DAAYDASLPFPVVRDRLPVLVPTPGLARRAAAVAARFGCDRVWFGAAAPLGLLAPALR
                     SAGIRRTVATTHGHEVWWARSPISRSLLHRIGETNDVLTYLGEFTRRRISAALSPAAA
                     ARMVRLTPGVDDEAFAPGCGGAAVRASLGLGDRPVVVCVSRIVARKGQDMLVRALPLI
                     RRRVPDTALLLVGDGPHRRRVESIVDSLGLRQSVVLTGAQPWSALAPYFDAGDVFAMP
                     TRTRRAGFEVEGLGIVYLEAAATGLPVVAGSSGGAPDAVLDGESGFVVNGRSVEAIAY
                     RVSQL"
     gene            complement(1041..>2265)
                     /locus_tag="VLC50_07500"
     CDS             complement(1041..>2265)
                     /locus_tag="VLC50_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020552984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HSF27343.1"
                     /translation="WNPLGARPPGGGVPVDPGADFTAAQVAREDAFHAALRPWSLSAL
                     AVGVLVPVGLALTPWGARLVTALARPLGGGWVWQVLLGVLGLTLLGRLASLPLSARAE
                     VVLRRYGLSTQTWSSWLVDAAKSWAVSTGLLLVVALVFYAVARATPHWWVWLSGAAAV
                     LVLVVSFAYPVVVEPLFNKFTPMPAGQLRDSLVQMARTDGVPVRDVLVADASRRTTAL
                     NAYVSGFGATRRIVVYDTLLSGAPPEQVRLVVAHELGHAKANDVLYGSLVGALAAAAA
                     VPLLALVLGSGPLLRRAGATGAGDPRSLALLLATVAVVSLLTQPGQMLVSRHIEARAD
                     VHALDLTRDPDTFVQMQRELAVTNLSDLDPPAPIYLWFGSHPTGPERTAMARDWALLH
                     GQPVPPPLAAPAATR"
BASE COUNT          359 a          854 c          844 g          208 t
ORIGIN      
        1 aacagctgcg acacccggta ggcaatcgct tctaccgagc gcccgttgac gacgaagccg
       61 gactcgccgt cgagcaccgc gtcgggcgct cccccagacg agccggccac caccggcagc
      121 ccggtcgccg cggcctcgag gtagacgatg cctagcccct cgacctcgaa cccggcccga
      181 cgcgtgcggg tcggcatcgc gaacacgtcg ccggcgtcga agtagggcgc cagcgccgac
      241 cacggctggg cgccggtgag caccaccgac tggcgcaggc ccagcgagtc gacgatcgac
      301 tcgacccgcc gccggtgggg cccgtcaccg acgagcagca gcgccgtgtc gggcactcgc
      361 cgtcggatca gcggcagcgc ccgcacgagc atgtcctgtc ccttgcgggc cacgatccgc
      421 gagacgcaga cgacgaccgg ccggtcaccc agcccgagcg aggcccgcac cgcggctcca
      481 ccgcagccgg gtgcgaacgc ctcatcgtcc acgcccgggg tcaaccggac catccgcgcc
      541 gccgccgcgg gcgagagcgc cgcggagatg cgccggcggg tgaactcccc caggtaggtc
      601 agcacgtcgt tggtctcacc gatgcggtgc agcagcgacc gggagatcgg cgagcgggcc
      661 caccacacct cgtgcccgtg ggtggtcgcg acggtacgac ggatgccggc tgagcgcagc
      721 gcgggggcaa gcagcccgag cggggcggcc gcgccgaacc agacccggtc gcagccaaac
      781 cgcgcggcca ccgcggcggc ccgacgcgcc agaccggggg tgggcacgag caccggcagc
      841 cggtcgcgca cgaccgggaa cggcagcgac gcgtcgtacg ccgcatcgcc gggctgacgc
      901 gcggtgagca ccaccaccga gtcggccggc atccgcgtcg tcatggccag cacgaaggac
      961 tcgatgcccc cggtgcgcgg cgggaagtcg ttggtgacga ccagggtccg ggcgggcacg
     1021 ggccagagcc tacggcccgg tcagcgcgtc gcggcgggcg cggccagcgg cggcggcacc
     1081 ggctgcccgt gcagcagtgc ccagtcgcgg gccatcgcgg tgcgttcggg cccggtgggg
     1141 tgcgagccga accacaggta gatcggcgcg ggcgggtcga ggtcggacag gttggtgacg
     1201 gccagctcgc gctgcatctg cacgaacgta tccgggtcgc gggtcaggtc gagggcgtgc
     1261 acgtcggcgc gcgcctcgat gtgccggctc accagcatct gcccgggctg ggtcagcagc
     1321 gacaccaccg cgacggtggc cagcagcagg gccagcgacc gcgggtcgcc agccccggtg
     1381 gccccggccc gacgcagcag cgggccggag ccgagcacca aggccagcag cggtacggcg
     1441 gcagccgcgg ccaacgcgcc gacgaggctg ccgtagagca cgtcgttggc cttggcatgg
     1501 cccagctcgt gggcgaccac caagcggacc tgctcgggcg gcgccccgga cagcagcgtg
     1561 tcgtagacga cgatccggcg ggtggcgccg aagccggaga cgtaggcgtt gagcgccgtc
     1621 gtacgccggg aggcgtcggc caccagcacg tcgcgcaccg gcaccccgtc ggtgcgggcc
     1681 atctgcacca gcgagtcgcg cagctgtccg gccggcatcg gggtgaactt gttgaacagc
     1741 ggctcgacga cgacgggata ggcgaacgag accacgagca cgagtacggc ggccgccccg
     1801 gacagccaca cccaccagtg cggggtggct cgggccacgg cgtagaagac cagggcgacc
     1861 accagcagca gcccggtcga gaccgcccac gacttggccg catcgaccag ccaggagctc
     1921 caggtctggg tggacaggcc gtagcggcgc agcaccacct cggcccgtgc cgacaacggc
     1981 agcgaggcca gccggcccag cagggtcagc ccgagcaccc ccagcagcac ctgccagacc
     2041 cacccgccgc cgagcgggcg ggccagcgcg gtgaccagcc gggcacccca cggggtcagc
     2101 gccagcccga ccggcaccag caccccgacc gccagcgcgg acagcgacca cgggcgcagc
     2161 gcggcgtgga aggcgtcctc gcgggccacc tgtgcggcgg tgaagtcggc acccgggtcg
     2221 accggcaccc cgccgcccgg cggtcgggcg cccagcgggt tccac
//
LOCUS       DATIBB010000189         7128 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_388296, whole genome shotgun sequence.
ACCESSION   DATIBB010000189 DATIBB010000000
VERSION     DATIBB010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 7128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7128
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_388296"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1232)
                     /locus_tag="VLC50_07505"
     CDS             complement(<1..1232)
                     /locus_tag="VLC50_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015490122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSF27344.1"
                     /translation="MRAQGWGWRHAGRRAFAVRGLDLRIEPGERLLLLGPSGAGKSTL
                     MLGLAGLLGREHEGEAEGSLEVAGVPALASRGRAGLLLQDPEAQVVLARCGDDVAFGL
                     ENFGVRREQIWPRVDEALDAVGFPFGRAHPTAALSGGEKQRLALAGVLAMRPGLLLLD
                     EPTANLDPPGAALVRGTVRRVVEATGATLVVVEHRVAAWLDLVDRMVVLEPGGGVVAD
                     GAPETVIKEYGAALAARGVWVPGHHPPGPARRPDPARGLDRPLLHADALAVARPGMRV
                     PAASGINLTLGAGRATAVVGPNGAGKSTVALTVAGLLAPRGGAVRAEPTLAGALVGPP
                     HRWRARDLLARLGTVFQDPEHQFLAPTVEDELRHGPRLLGLPDAAVQARADELLDRLR
                     LAHLGAASPYTLSGGEKRR"
     gene            complement(1331..2116)
                     /locus_tag="VLC50_07510"
     CDS             complement(1331..2116)
                     /locus_tag="VLC50_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019181390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HSF27345.1"
                     /translation="MSTTASKSSQPTATPARGTARRGVAAGVLGVVSAVIFAIGLHGD
                     AWDPFDVLIAALFGVGVGVVIWAAASGPRWRVVDIVVASVLGVASGVIFWAWGIAWTP
                     LSGALAFFAPLSGLLAGGWLFAGVLGGLVIRKPGAALYTETVAAVVSMLIGTQWGFST
                     LIWGVLEGLGAEIILALLLYRRWGLPTALLAGLGAGVAVGILDTTFTGYVTWSAGWRL
                     AYAGSAVVSGIVVAGLLSWLAVRALARTGVLAPFAAGREAREV"
     regulatory      complement(2220..2327)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            2421..2726
                     /locus_tag="VLC50_07515"
     CDS             2421..2726
                     /locus_tag="VLC50_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006593833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4235 domain-containing protein"
                     /protein_id="HSF27346.1"
                     /translation="MLNLGWKLTGGLGAVVAGIAARKAITGLWKTATGNPPPDSPEDP
                     DTDWREALGWAVASGAAIGVARLVATRKATQYYRRSTGHLPGQPRAEQSQPAQPAPV"
     gene            2736..2888
                     /locus_tag="VLC50_07520"
     CDS             2736..2888
                     /locus_tag="VLC50_07520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF27347.1"
                     /translation="MLRAMRDQTGAPSKAIRVLAVIVVIGMIALASPVIPAVLAPALR
                     WFLGVL"
     gene            complement(2896..4068)
                     /gene="hutI"
                     /locus_tag="VLC50_07525"
     CDS             complement(2896..4068)
                     /gene="hutI"
                     /locus_tag="VLC50_07525"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012924313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HSF27348.1"
                     /translation="MPTVLLTGIGELVTTDPTQGDGSPLGLVRDAALVIDGSHVAWVG
                     PASDAPAADSSYDLGGRAVIPGFVDSHAHLVFAGDRAQEFAARMAGTPYGAGGIATTV
                     AATRAATDEQLAANTARLVAEMLRQGTTTVEIKSGYGLSVRDEARALAIARAFTDETT
                     FLGAHVVPSEYAEDPAAYVDLVTGPMLDAAAPHARWVDAFCDRGAFDADQARAVLTAG
                     MAAGLAPRLHANQLGPGPGVQLACELGAASADHCTHLTDADVAALADSGTVATLLPGA
                     EFSTRSPWPDARRLLDAGVTVALATDCNPGSSYTSSMPFCLALAVREMRMSPSEALWS
                     ATAGGARALRRDDVGTLRVGARADLAVLDAPSYIHLAYRPGVPLVSAVWQAGRPVG"
     gene            complement(4068..5426)
                     /locus_tag="VLC50_07530"
     CDS             complement(4068..5426)
                     /locus_tag="VLC50_07530"
                     /EC_number="3.5.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018157785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamate deiminase"
                     /protein_id="HSF27349.1"
                     /translation="MSEVFCCDYAWLPDGVHADVDIEVEAGRIVAVRPHDAAAPPNAH
                     DQCGEGGGRGVAGLVLPGFANVHSHAFHRALRGRTHDGRGSFWTWRERMYAVAGRLDP
                     DSYYALARATYAEMVLAGISCVGEFHYLHHQPGGAPYAEPNAMGEALRAAARDAGIRL
                     TLLDTCYLAGGLDPEGHSPLTAPQRRFGDGDALRWAARVAALQPDATTKIGAAIHSVR
                     AVPRDQLSDVVEAAEGRPLHVHLSEQPAENAAARAAYGLTPTQLLDEAGALGPETTVV
                     HATHLERADVRRLGASRTAACFCPTTERDLADGIGPARALADAGASLCLGSDQHAVID
                     PLEEARALEMHERLASGERGRLTVEELVDALTWRGHAALGWVDAGRIEVGARADLVAL
                     RLDTARTAGSAPEQAVLAATAGNVDTVIVEGKVIVEAGRHVTLDVARELAAAITPLWQ
                     DA"
     gene            complement(5521..6738)
                     /locus_tag="VLC50_07535"
     CDS             complement(5521..6738)
                     /locus_tag="VLC50_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006605198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016813 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear
                     amidines [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate amidohydrolase"
                     /protein_id="HSF27350.1"
                     /translation="MTTYDQLWGSLEPIGRDSATGGYERFAYAGADLAAREWLVAAAQ
                     ERGLDVHTDRNGNLWAWATPPGPDALVVGSHLDSVPQGGAYDGPLGVISALAALDALR
                     ERALTLKRPLAVVVFADEEGARFGTACVGSRLLTGALDPDTARGLRDRDGTTMAEAMR
                     AAGHDPTHLGRDLETLRGIGAFVELHVEQGRALVALDAPVGVATRIWPHGRYRFTFTG
                     QANHAGTTRMDDRHDPMLTYAMTALAANKQARLADARATFGKVAVVPNGTNAIPSRVD
                     GWLDARARDDEALELLLDAVRRQAYDRADRDGTTLEVFPESASPAVEFDADLRDRLAL
                     LLGAPDGEPAPVLATQAGHDAGILAAAGVPAAMLFVRNPTGVSHSPAEHAERDDCLAG
                     VAALARVIEDLAC"
     gene            complement(6862..>7128)
                     /locus_tag="VLC50_07540"
     CDS             complement(6862..>7128)
                     /locus_tag="VLC50_07540"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020580340.1"
                     /note="catalyzes the formation of
                     4-imidazolone-5-propanoate from urocanate during histidine
                     metabolism; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HSF27351.1"
                     /translation="NAMVNVASGATWVSIHHGGGVGIGRSIHAGQVTVADGTPLAAEK
                     INRVLTNDPGMGVIRHVDAGYDRATEVAAERAVRIPMRESPTLH"
BASE COUNT          933 a         2668 c         2689 g          838 t
ORIGIN      
        1 cggcgcttct cgccgccgga gagcgtgtag gggctggccg cgcccaggtg ggccagccgc
       61 agccggtcga gcagctcgtc ggcgcgagcc tgcaccgcgg cgtctggcag gccgagcagc
      121 cggggcccgt ggcgcagctc gtcctccacg gtcggggcga ggaactggtg ctcggggtcc
      181 tggaacacgg tgccgagccg ggccagcagg tcgcgggccc gccaccggtg cggtggaccg
      241 accagtgcac cggccagggt gggttcggca cgtacggcgc ccccgcgcgg tgcgagcagc
      301 ccggcgacgg tcagcgccac cgtcgacttg ccggcgccgt tgggcccgac caccgcggtg
      361 gcccggccgg cgccgagggt caggttgatc ccgctggcgg ccgggacccg catgcccggg
      421 cgggccaccg ccagggcgtc cgcgtgcagc agcggccggt ccagcccgcg ggccgggtcg
      481 ggccggcggg ccgggccggg cgggtggtgt cccggcaccc acacgccgcg ggcggccagc
      541 gccgcgccgt actccttgat gaccgtctcg ggggcgccgt cggcgaccac gccgccgccg
      601 ggttcgagca ccaccatccg gtcgacgagg tcgagccagg cggccacccg gtgctcgacc
      661 acgaccaggg tggccccggt cgcctcgacc acccgacgca ccgtgccccg caccagcgcc
      721 gccccgggcg ggtcgaggtt ggcggtcggc tcgtcgagca gcagcaggcc ggggcgcatg
      781 gcgagcaccc cggccagggc cagccgctgc ttctcgccgc cggagagcgc cgcggtcggg
      841 tgggcccggc cgaacgggaa acccaccgcg tccaacgcct cgtcgacgcg cggccagatc
      901 tgctcgcgtc gtacgccgaa gttctccagc ccaaacgcga cgtcgtcgcc gcagcgggcc
      961 agcaccacct gggcctcggg gtcctgcagc agcagcccgg cccgcccgcg cgaggccaac
     1021 gccggcacgc ccgcgacctc gaggctgccc tcggcctcgc cctcgtgctc gcggccgagc
     1081 agcccggcca gcccgagcat cagcgtggac ttgccggcgc cggacgggcc gagcagcagc
     1141 agccgttcgc cgggctcgat gcgcagatcg agcccgcgta cggcgaacgc gcgccggccg
     1201 gcgtggcgcc agccccagcc ctgcgcgcgc accccgaccg gggcgacggg cccggggtcg
     1261 gggggcgcga cggtcgcgct ggggtgagcg gccacggagg cgcgacgaga ggcgaacacg
     1321 gtgaccgcga tcagacctcg cgggcctcac gcccggccgc gaacggcgcg agcaccccgg
     1381 tgcgggccag cgcgcgcacc gccagccagg acagcagccc ggccaccacg atcccggaga
     1441 cgaccgcgga gccggcgtag gccagccgcc agccggccga ccaggtgacg tagccggtga
     1501 acgtggtgtc gaggatgccg accgccacgc cggcgcccag cccggccagc agcgcggtgg
     1561 gcaggcccca gcggcggtag agcagcagcg cgaggatgat ctcggcgccc agaccctcga
     1621 gcacgcccca gatcagggtg gagaagcccc actgggtgcc gatcagcatc gacacgaccg
     1681 cggcgaccgt ctcggtgtag agggcggcgc ccggcttgcg gatcaccagc ccgccgagca
     1741 cgccggcgaa cagccagccg ccggcgagca gcccggacag cggggcgaag aatgccaggg
     1801 cgccggacag cggggtccag gcgatgcccc aggcccagaa gatcacgccg ctggccacgc
     1861 cgagcacgct ggcgacgacg atgtcgacga cccgccagcg gggcccgctt gcggccgccc
     1921 agatcacgac gccgacaccg acgccgaaca aggcggcgat cagaacgtcg aacggatccc
     1981 aggcgtcgcc gtgcagcccg atcgcgaaga tcacggcaga gacgacgccg agcactccgg
     2041 cggcgacccc ccgtcgggcg gtgccgcgcg cgggcgtcgc ggtgggctga gatgactttg
     2101 atgcggtggt gctcacgccg aacctcctca gggtccgacg gggggcccaa cgggtgggcc
     2161 gaggcacccg gtgcgcggcg tacgacggaa ggcgcgcgag cacgccgtac gtcgacgtca
     2221 cgaccggggg accgagctcc ctgcgccggc atgacccgga tcaggttcta gggtctgcgg
     2281 atctccgcac tctcagcgca cttgcgtgcg ctcccctgtc agtcagtgga cgcggatcac
     2341 gctagcaccg tgcgcgaaga gcgcccagag ggcagggtgg agatcggcac cacatccgac
     2401 ggaagagggc ggaccagacg atgctcaacc tgggctggaa gctgaccggc gggctgggcg
     2461 cggtcgtcgc cggcatcgcg gcccgcaagg cgatcaccgg gctgtggaag accgcgaccg
     2521 gcaacccgcc gccggacagc cccgaggatc ccgataccga ttggcgggag gcgctgggct
     2581 gggcggtggc ctccggggcc gcgatcgggg tcgcccggct ggtggccacc cgcaaggcga
     2641 cgcagtacta ccgccggtcc accggccacc tgcccggcca gccccgcgct gagcagtcgc
     2701 agccggcgca accggcgccc gtctaggctg cacccatgct gcgcgcgatg cgcgaccaga
     2761 ccggagcgcc gtccaaggcg atccgggtgc tcgccgtgat cgtcgtcatc ggcatgatcg
     2821 cgttggcgtc gccggtgatc ccggcggtgc tggccccggc gctgcgctgg ttcctcggcg
     2881 tcctctgaga ttgcgtcagc cgacggggcg accggcctgc cagaccgcgc tcaccagcgg
     2941 cacccccggc cggtaggcca gatggatgta cgacggtgcg tcgagcaccg cgaggtcggc
     3001 gcgggccccg actcgcagcg tgcccacgtc gtcgcggcgc agcgcccttg ccccgccggc
     3061 cgtcgccgac cagagggcct ccgaggggct catccgcatc tcgcgcaccg ctagcgccag
     3121 gcagaacggc atcgacgacg tgtaggacga gccggggttg cagtcggtgg ccagcgccac
     3181 ggtcaccccg gcgtcgagca gccggcgcgc gtccggccac ggcgaccggg tggagaactc
     3241 ggctcccggc agcagggtgg ccaccgtgcc cgagtcggcg agcgcggcca cgtcggcgtc
     3301 ggtcaggtgg gtgcagtggt ccgcgctggc cgcacccagc tcgcaggcca gctggactcc
     3361 agggccaggg ccgagctggt tggcgtgcag ccgcggggcc agcccggcgg ccatccccgc
     3421 cgtgagcacc gcccgggcct ggtcggcgtc gaacgcgccc cggtcgcaga acgcgtcgac
     3481 ccagcgggcg tgcggcgcgg cggcgtcgag catcggcccg gtcaccaggt cgacgtacgc
     3541 cgccgggtct tctgcgtact cgctcgggac cacgtgcgcg ccgaggaagg tggtctcgtc
     3601 ggtgaacgcg cgcgcgatcg ccaacgcccg ggcctcgtcg cgcacgctca gcccgtagcc
     3661 gctcttgatc tccaccgtgg tggtgccctg gcgcagcatc tcggcgacca gccgggcggt
     3721 gttcgcggcc agctgctcgt cggtggccgc ccgggtcgcg gcgacggtgg tggcgatgcc
     3781 gccggcgccg tacggcgtgc cggccatccg ggcggcgaac tcctgcgccc gatcgccggc
     3841 gaagaccagg tgggcgtggc tgtccacgaa cccggggatg acggcccggc cccccaggtc
     3901 gtacgacgag tccgcggccg gcgcgtcgct ggccggcccg acccaggcca cgtgcgagcc
     3961 gtcgatgacc agcgcggcgt cgcggaccag gcccagcggt gaaccgtcac cctgggtcgg
     4021 gtcggtggtg accagctcgc cgatgccggt cagcagcacg gtcggcatca ggcgtcctgc
     4081 cagagcgggg tgatcgccgc ggccagctca cgcgccacgt cgagggtgac gtgccggccg
     4141 gcctcgacga tcaccttgcc ctcgacgatc acggtgtcga cgttgcccgc ggtggcggcc
     4201 agcaccgcct gctcgggcgc ggacccggcg gtgcgggcgg tgtcgaggcg gagcgcgacc
     4261 aggtcggcgc gggctccgac ctcgatgcgg ccggcgtcga cccagcccag cgcggcgtgc
     4321 ccgcgccagg tcagcgcgtc gacgagctcc tcgacggtga gccggccgcg ttcgccggag
     4381 gccagccgct cgtgcatctc cagcgcccga gcctcctcga gcgggtcgat caccgcgtgc
     4441 tggtccgagc ccaggcacag cgaggcccct gcgtcggcca gtgccctcgc cgggccgatc
     4501 ccgtcggcca ggtcgcgctc ggtggtcggg cagaagcagg ccgccgtacg gctggcgccc
     4561 aggcgtcgta cgtcggcccg ctccaggtgg gtggcgtgca cgaccgtggt ctcggggccg
     4621 agcgcgccgg cctcgtcgag cagctgtgtc ggggtgagcc cgtaagcggc gcgcgcggcg
     4681 gcgttctcgg ccggctgctc ggacaggtgc acgtgcagcg ggcgtccctc agccgcctcc
     4741 accacgtcgg agagctggtc gcgcggcacc gcccgcaccg agtggatagc cgcgccgatc
     4801 ttcgtggtgg catcaggctg cagcgcggcg acccgggcgg cccagcgcag cgcgtcgccg
     4861 tcgccgaacc ggcgctgcgg cgcggtcagc gggctgtgcc cctccgggtc gagcccgccg
     4921 gcgaggtagc aggtgtcgag cagggtcagc cggatcccag cgtcacgagc ggccgcgcgc
     4981 agcgcctcac ccatggcgtt gggctcggcg tagggcgccc cgccgggctg gtggtgcagg
     5041 tagtggaact cgccgacaca gctgatgcct gcgagcacca tctcggcgta cgtcgccctt
     5101 gccagcgcgt agtagctgtc ggggtcgagc cggccggcga cggcgtacat ccgctcgcgc
     5161 caggtccaga acgagccgcg cccgtcgtgg gtgcgcccgc gcagggcccg gtgaaacgcg
     5221 tgcgagtgca cgttggcgaa cccgggcagc accagtcccg ccacccccct gccgccgccc
     5281 tccccgcact gatcatgcgc gtttggcggg gctgcggcgt cgtgagggcg tacggcgacg
     5341 atccggccgg cctcgacctc gatgtcgacg tcggcgtgca ccccgtcggg cagccaggcg
     5401 tagtcgcagc agaacacctc gctcacgccg accccctcgc ccccctcggc acccctgcac
     5461 tgatcatgcg cgtttggcgg gccgccaaac gcgcatgatc agtgcagaga tgctgggcgg
     5521 tcagcatgcg aggtcctcga tgacccgggc cagcgcggcc accccggcca ggcagtcgtc
     5581 gcgctcggcg tgctcggccg gcgagtgcga gacgccggtc gggttgcgca cgaaaagcat
     5641 cgcggccggc acccccgcgg cggcgaggat gccagcgtcg tgcccggcct gggtcgccag
     5701 caccggcgcc ggttccccgt cgggcgcgcc gagcagcagc gccagccggt cgcgcaggtc
     5761 ggcgtcgaac tcgacggccg ggctggccga ctcgggaaac acctcgagcg tggtcccgtc
     5821 gcggtcggcg cggtcatagg cctgtcgccg tacggcgtcc agcagcagct cgagcgcctc
     5881 gtcgtcgcgg gcccgcgcgt cgagccagcc gtcgacccgg gacgggatcg cgttggtgcc
     5941 gttgggcacc accgcgacct tgccgaacgt cgcccgggcg tcggcgagcc gggcctgctt
     6001 gttggcggcc aacgcggtca tcgcgtaggt gagcatcggg tcgtgccggt cgtccatccg
     6061 ggtggtgccg gcgtggttgg cctggccggt gaacgtgaac cgatagcgcc cgtgcggcca
     6121 gatccgggtg gctaccccga ccggcgcgtc gagcgcgacc agcgcgcggc cctgctcgac
     6181 gtgcagctcg acgaatgccc cgatccctcg cagcgtctcg aggtcgcgac ccaggtgcgt
     6241 cgggtcgtgc ccggccgcac gcatcgcctc ggccatcgtg gtcccgtcgc ggtcgcgcag
     6301 cccgcgggcg gtgtcggggt cgagcgcgcc ggtgagcagc ctggacccca cgcacgcggt
     6361 gccgaaccgg gcgccctcct cgtcggcgaa caccaccacc gcgagcggcc gcttgagggt
     6421 gagagcccgc tcgcgcagcg cgtcgagggc ggccaacgcg ctgatgacgc ccagcggccc
     6481 gtcgtacgcg ccgccttggg ggaccgagtc gaggtgcgag ccgaccacca gcgcgtccgg
     6541 gccgggcggg gtggcccagg cccacaggtt gccgttgcgg tcggtgtgca cgtcgaggcc
     6601 gcgctcctgc gccgccgcga ctaaccactc ccgggcagcc aggtcggcac cggcgtacgc
     6661 gaaccgctcg tagccgccgg tggccgagtc acgcccgatc ggctcgagtg agccccacag
     6721 ctggtcgtac gtcgtcatct cgcgccctcc tctcgcccga cactccgcga atgatcatgg
     6781 tcgacgccgt cgagcccgag cgatggtttg cgcatgatcg ctgcaggaga ggcagcagtc
     6841 aggcccgcca aacgcgcatg atcagtgcag ggtgggggac tcgcgcatcg ggatgcgtac
     6901 ggcgcgttcg gcggccacct cggtggcgcg gtcgtagccg gcgtcgacgt gacggatcac
     6961 gcccatgccc gggtcgttgg tgagcacccg gttgatcttc tcagcggcca gcggcgtgcc
     7021 gtcggccacg gtgacctgcc cggcgtggat cgaccggccg atgccgaccc cgccgccgtg
     7081 gtggatcgac acccaggtcg cgccggaggc cacgttgacc atcgcgtt
//
LOCUS       DATIBB010000190         2971 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_1834665, whole genome shotgun sequence.
ACCESSION   DATIBB010000190 DATIBB010000000
VERSION     DATIBB010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2971)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2971)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2971
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1834665"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            383..1654
                     /locus_tag="VLC50_07545"
     CDS             383..1654
                     /locus_tag="VLC50_07545"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HSF27352.1"
                     /translation="MRRVPALGALLLAASAVSGCGVLGDDGGATSRPAAQSGRHSADS
                     TPSPTVDPDAPSGWGPTMGEIEEARGTVAAMSLEDKVATVLMPGFWGYDGADPTAAEA
                     AQNQLMHQVDSPVEVAEAQPYGGFFLRPEVIGDADQVARLAQQLHDAGDGPDGLALLV
                     AMDQEGGVVQRLKEGVDVVPGAATVGATGDPAYARKVARQNGGTLRQLGVTMVLAPVA
                     DVDPDGSSVIGSRAYSRDPDEAAEMVVASVEGYLQAGVIPTVKHFPGLGTVSGDSHRS
                     LPVQRKPVDELMRTDLVPFRAAIDAGAPVLMSAHVAVAALEKDMPASLSRPVLEGLLR
                     DRLGFDGVIITDSQGMGPIHGRFGPAEGAVRSLLAGNDLVLNSFRPEKARRAVIRAVE
                     SGRLAEERVAEAATRVEALRLYQQRLASSAD"
     gene            complement(1656..2471)
                     /locus_tag="VLC50_07550"
     CDS             complement(1656..2471)
                     /locus_tag="VLC50_07550"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020540682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HSF27353.1"
                     /translation="MSGERDTDGARTFVAMGDSFTEGLEDELGPFGRHLGWADRVAAA
                     LATRDGGLRYANLAVRGRLLDQVVDEQLPIALGLSPDLVTFHAGPNDVLRPRADVPAV
                     LRRYAAAVASLRGTGVEAVLFTVIERAGGTGRTAQRLADRFAAFNAGVRDVARRHGAL
                     LVDQGAVPALQDRRLWHEDRLHLAPEGHARVAAAVLERLGVDDPALLGGEPGWWQVPL
                     APGARPRRRADLVADVRWVRRYFLPWVGRRLRGVSSGDRLTAKHAELVDVLPK"
     gene            2702..>2971
                     /locus_tag="VLC50_07555"
     CDS             2702..>2971
                     /locus_tag="VLC50_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016435342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF27354.1"
                     /translation="MGSVVAAEARLAAEAPRVHPLVADGPLALLITGLTYASFVGVGT
                     PEEGDRLHPADSFGAMLLLAQTVPLVWRRRAPLLVFTVVCLAFLAF"
BASE COUNT          386 a         1123 c         1108 g          354 t
ORIGIN      
        1 ggctcgagct cgtccgtcac gggtggaccg ttgccccagc ccggcgcgcc gcgcaggcac
       61 cgacccccag gtgtggaggg cgcctcggcc gggcgcggcc tgttgacaca cgagcgaacg
      121 cgccggtcag caacgccgcg ggcgacgtac ctgaccgggt ggccggcgtg tctgccggcg
      181 tgtcggcacg agacgtcgcc cacgaccgag ggggcgcggc agcaggtccc gcggttagcc
      241 aggaacccgg cggtcagcac gaacagcccg gagtcgggat cgggaagccg gcgtccccgc
      301 gcgcggtcgc cgccgcatgc cgacgagccc aggtcccggc gatgacagcg gccgacgggc
      361 cggatggcac gatgggcggg cgatgcgacg agttccggcc ctgggggcac tcctgctggc
      421 cgcctcggcg gtcagcggct gcggcgtcct cggcgacgac ggcggcgcaa cgagcaggcc
      481 cgcggcccag tccggccggc actcagccga cagcacgccc tcccccaccg tcgaccccga
      541 cgcgcccagc gggtggggac ccaccatggg cgagatcgag gaggcccgcg gaaccgtcgc
      601 ggccatgagc ctcgaggaca aggtcgccac cgtgctgatg cccggcttct gggggtacga
      661 cggcgcagac cccaccgcgg cagaggccgc gcagaaccag ctcatgcacc aggtcgactc
      721 ccccgtcgag gtggccgagg cgcagccgta cggcgggttc ttcctccgcc cggaagtcat
      781 cggagacgcc gaccaggtcg cccggctcgc ccagcagctg cacgacgcag gcgacggccc
      841 cgacggactg gcgctgctgg tcgccatgga ccaggagggc ggcgtcgtcc aacgtctcaa
      901 ggagggcgtc gatgtcgtgc ccggtgcggc caccgtcggc gcgaccggcg accccgcgta
      961 cgcccgcaag gtcgcccggc agaacggtgg cacgttgcgc cagctgggcg tgacgatggt
     1021 gctggcgcca gtcgccgacg tggaccccga cgggtcctcg gtgatcggct cccgggccta
     1081 ctcgcgcgac cccgacgagg cagccgagat ggtggtggcc agcgtggagg gctacctgca
     1141 ggccggcgtc atccccacgg tgaagcactt tcccggcctc ggcaccgtca gcggcgacag
     1201 ccaccggtcc ttgcccgtgc agcgcaagcc ggtggacgag ctgatgcgga ccgacctggt
     1261 gccgttccgc gcggcgatcg acgccggcgc gcccgtgctc atgagcgcgc acgttgcggt
     1321 cgccgcgctg gagaaggaca tgcccgcctc gctgagccgg ccggtgctcg aagggctgct
     1381 gcgcgaccgg ctcggcttcg acggggtgat catcaccgat tcgcagggga tgggcccgat
     1441 ccacggccgg ttcggcccgg cggagggcgc ggtgcgctcg ctgctcgccg gcaacgatct
     1501 cgtgctcaac tcgttccggc cggagaaggc ccgcagggcg gtgatccgtg ccgtcgagag
     1561 cggccggctc gccgaggagc gagtcgcgga ggccgcgacg cgggtcgagg cgctgcggct
     1621 gtaccagcag cggctggcgt cgtccgccga ctgacctact tcggcagtac gtcgaccagc
     1681 tcggcgtgct tggccgtcag gcggtcgccg ctcgacaccc cgcggaggcg tcggcccacc
     1741 cacggcagga agtaccgccg aacccaccgc acgtcggcga ccaggtcggc ccgccgccgg
     1801 ggtcgggcgc ccggcgccag cggcacctgc caccaccccg gctcgccgcc gagcagtgcc
     1861 gggtcgtcga cgccgagccg ctccagcacc gccgcggcga cccgcgcgtg cccctcgggc
     1921 gcgaggtgca gccggtcctc gtgccagagc cgccggtcct gcagcgccgg caccgcaccc
     1981 tggtcgacca gcagcgcgcc gtgccgccgc gccacgtccc ggacgccggc gttgaacgcc
     2041 gcgaaccggt cggcgaggcg ttgcgccgtc cgccccgtcc cacccgcccg ctcgatcacc
     2101 gtgaacagca cggcctcgac gccggtgccg cgcagggacg cgacggccgc ggcgtagcgg
     2161 cgcagcacgg cgggcacgtc ggcgcgcggg cgcagcacgt cgttcggccc ggcgtggaag
     2221 gtgaccaggt cgggcgagag gcccagggcg atcggcagct gctcgtcgac gacctggtcg
     2281 agcagccggc cgcggaccgc gaggttcgcg tagcgcaggc cgccgtcccg ggtggccagc
     2341 gccgcggcca cccggtccgc ccacccgagg tgccgcccga acggcccgag ctcgtcctcg
     2401 aggccctcgg tgaacgagtc gcccatcgcg acgaaggtgc gcgcgccgtc ggtgtctcgc
     2461 tcgccactca cgagccgtat cgtgcccgag cccctcaccg gatgcgccca ctgccggttg
     2521 cgtggcagtt cgtcccggtc gccagcggcc gacgaatggg ctatcacgga gagagggcaa
     2581 gccaggcctg gggtgccgcc ggcacggctt gctccggaga ggggcgcagc gaccggtcca
     2641 tcaggtcctc gcgtccgcac gcaggtgagg tctcgcgggc cggccgaggg gggtgacgca
     2701 catggggtct gtcgtcgctg ccgaggcccg gctggccgcc gaggcacctc gggtgcaccc
     2761 gctggtggcg gacggccccc tggcgctgct catcaccggt ctcacctacg ccagcttcgt
     2821 cggcgtgggc acgccggagg agggggaccg actccacccg gccgactcgt tcggagccat
     2881 gctgctgctc gcccagacgg tgcccctcgt gtggcgacgg cgcgcaccgc tgctcgtctt
     2941 caccgtcgtg tgcctggcgt tcctcgcctt c
//
LOCUS       DATIBB010000191        10694 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_1440907, whole genome shotgun sequence.
ACCESSION   DATIBB010000191 DATIBB010000000
VERSION     DATIBB010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 10694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10694
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1440907"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..327)
                     /locus_tag="VLC50_07560"
     CDS             complement(<1..327)
                     /locus_tag="VLC50_07560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF27355.1"
                     /translation="MFDNTGDGPTDDAIGSEPTDTSHTEVPGPAGPLAPADPLLPVDP
                     PAPAVPAEPPALPGLAGRLLAGLDPASAAAVRAAVSHERVPVDEEVTLPGWAGLPGSL
                     ALATAVA"
     gene            490..2394
                     /locus_tag="VLC50_07565"
     CDS             490..2394
                     /locus_tag="VLC50_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020573741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HSF27356.1"
                     /translation="MDTPLPHLDRLFDYLVPAELDGAAAPGVRVRVRFAGKLVDGFLL
                     ERVATSEHSGRLTPLASVVSEEPVLDPETLALSRLVAARYAGSLADVLRLAVPPRHAR
                     AERAPAPAPPTEPPPPTVVGPWDRYRHGVGFLDALRAGSAPRAVWTALPGEDWPTALA
                     VAARSTLDGGRGTLVVLPDHRDVARVDLAMGAQLGPGRHVVLTAELGPEARYRRWLQL
                     RRGQVKVVVGTRAAAFAPVADLGLVALWDDGDDLHAEPRAPYPHAREVLMLRAHHGGT
                     AALLAGFAVTAEAGAMVESGWARPVVAAREAVRAAAPRILAAGGDDELARDPAAAAAR
                     LPSLAWRTARAALATGPVLVQVPRRGYLPALACQTCRSPARCPSCQGALAMAQGRPAP
                     VCQRCGRVEPAWRCADCGGQRLRAAVIGARRTAEELGRAFPSVPVRTSGRDGVLARVD
                     PVPALVVSTPGAEPLVEGGYAAALLLDAWALLGRADLRAGEEALRRWLTAAALVRPAS
                     SGGQVVVLADAGLAPVQALVRWDPAGFASRELAERTALRLPPAVRVAELTGPAPDVEA
                     MLAAVALPPAAEVLGPVPAEESTVRVLVRVDKRDGLALASALHAAAGSRSARKQGAHV
                     RIRIDPADLG"
     gene            2757..3302
                     /gene="def"
                     /locus_tag="VLC50_07570"
     CDS             2757..3302
                     /gene="def"
                     /locus_tag="VLC50_07570"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012889527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HSF27357.1"
                     /translation="MAIKPIRLFGDPVLRTPAAPVVDFDKELRQLVADLTDTMRDAPG
                     AGLAAPQLGVSLRVFTYWVDDELGHLVNPSLDLSDEEQDGEEGCLSFPGLYFDTKRAL
                     RVVAKGQDMYGEPVVIEGSDLLARCVQHETDHLDGVLFIDRLDRETRKLALKAIREAE
                     WSEGAPPVVKTSPHPTFGKAI"
     gene            3306..4259
                     /gene="fmt"
                     /locus_tag="VLC50_07575"
     CDS             3306..4259
                     /gene="fmt"
                     /locus_tag="VLC50_07575"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017949520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HSF27358.1"
                     /translation="MRLVFAGTPEVAVPSLDALLASRHDVVAVLTRPDAPAGRGRTVV
                     ASPVARRAEAAEIPVLKPTRLRDEAFLDTLRALAPDAAPVVAYGALVPPAALTIPRHG
                     WVNLHFSLLPAWRGAAPVPHALLAGDEVTGATTFRLDEGLDTGPTYGCLTEAVGAHDT
                     AGDLLDRLAEAGAALLVATLDALEDGTIEARDQPVEGVSFAPKVTVEQARVDWSAPAF
                     AVDRLVRAMTPHPGAWTTWRGQRLGLGPLEPADDAGLAPGDVRVTRREVLVGTGTAAV
                     RLHEVRPAGRRPMPAVDWARGARPQPGERLGNPTEDEVDAR"
     gene            4249..5664
                     /locus_tag="VLC50_07580"
     CDS             4249..5664
                     /locus_tag="VLC50_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010263379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HSF27359.1"
                     /translation="MPASARTRRSRPARTDPARRVAYDLLRAVDEHDSYANLALPALL
                     RQRGLHGRDAALATELAYGTLRGRGTYDAVLAACVDRPLDQLDPPVLDVLRLGAHQLL
                     ETRIPPHAAVASTVALAREALTAGPAGLVNAVLRRVAEHDLPTWLARLAPDAEADPDG
                     HLAVTTSHPAWVVRALRDALRADGRDPAELARLLAADNRAPAVTLVARPGRASRDELV
                     AAGANPGRWSPYAAVLPAGDPAGVPAVAAGRAGVQDEGSQLVALMLRAAPLAGPDTRW
                     ADLCAGPGGKAALLAALADPVGAELVAVESAAHRADLVRRALTGTPGRWRVEVGDGRA
                     VGAAVTDGAVQPYDRVLVDAPCTGLGALRRRPEARWRKTPADVATLTRLQRELLGSAL
                     DAVRPGGVVAYATCSPHVAETRVVVDDVLRRRGDVEALDAGAVLRDVAPDLPLGPGPY
                     AQLWPHVHRTDAMFVALLTRR"
     gene            5675..6349
                     /gene="rpe"
                     /locus_tag="VLC50_07585"
     CDS             5675..6349
                     /gene="rpe"
                     /locus_tag="VLC50_07585"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014056277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HSF27360.1"
                     /translation="MGVQISPSILSADFANLERELARVANADWVHVDVMDNHFVPNLT
                     LGLPVVERLVAVSPRPVDVHLMIDDPDRWAPAYAEAGAHSVTFHVEAAAAPVRLAREL
                     RAAGSRAAMALRPATPVEPYADLLPELDMLLLMTVEPGFGGQPFLDVVLPKIRRARAI
                     IERAGIEVWLQVDGGVSSETIERAAAAGADVFVAGSAVYRAEDAAAAVDELRALAEKA
                     AGAPPA"
     gene            complement(6429..6902)
                     /locus_tag="VLC50_07590"
     CDS             complement(6429..6902)
                     /locus_tag="VLC50_07590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF27361.1"
                     /translation="MTPASLTMSAQPTRRTGGGPHGAGRSDDFASTEVIRPAAVLSEA
                     DARRILAGLARDDVRNSGHWWTRVGVWRRYDKPWELGSDEPGEAVHLGSISCVYDSPT
                     RYAVTVFRVSLTSYGLRHGWTTERLCDDAFRHADLTLSSCPRAQLAPPPRPLRWS"
     gene            complement(6899..8956)
                     /locus_tag="VLC50_07595"
     CDS             complement(6899..8956)
                     /locus_tag="VLC50_07595"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HSF27362.1"
                     /translation="MTTATDRSVSDAADVPHTDADLVLAMRLDGHVLSASTTVETVLG
                     WDLELLQREGIAACATEHDLPVLRAFGSRLGEAGSLRSTIRLDGADGGTVWLDVAGKL
                     FATEPDGTYAYLSARDVTDDVLAMTHLAASEHQWRLAFEHSPIGGALVAPDGGFLLVN
                     DALAAMLDYRAEELTRSTVRSVTAPEDWSAMWLHWQELLEGRRATYAADHRYLTANGR
                     MLWCHLTAAPVLDDDGDLHAVMIQLQDITERRDAELALASQALHDALTGLPNRFMTRQ
                     WLASALGDHPGQPVGVLYCDLDRFKLVNDSLGHACGDELLVEVSQRLRDALRDDDLVG
                     RVGGDEFIVVAERLDGPDDLLGVAARLVRALTEPVRVGGHPHTVTLSIGAAIGQDPET
                     AEDLLVRADMALLRAKRLGRARIELFDVERDRIATRDDLELEDNLRESVEAGELRAHY
                     QPIVSLREERLVGYESLLRWQHPARGLLAPEVFLDLAESSGLISSIGWWMLARVCSDA
                     TAPGARLCANGTWVSVNASPSQLSRPGVAGVVRRTLEESGVRPEQLHLEVTETALIQA
                     SGALADELREISGMGVRIALDDFGTGYSSLSLLRDFPVDMVKIDRSFVQPLLHDRSAH
                     AIVRAVLSMCHDLGLPTVAEGVETEAQADRLRELGCSHAQGFLFGRPQPLPPLPSLGK
                     DPR"
     regulatory      9078..9208
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            9330..10358
                     /gene="ribD"
                     /locus_tag="VLC50_07600"
     CDS             9330..10358
                     /gene="ribD"
                     /locus_tag="VLC50_07600"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004952259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HSF27363.1"
                     /translation="MATTAELAAMRRALELAEIPGGPPATNPRVGAVLLSPDGRVLGE
                     GWHRGAGTPHAEVEALAAAGESARGATAVVTLEPCDHVGRTGPCSQALIDAGIARVVY
                     AMADPNPVAGGGAGRLRAAGVEVEAGPLAEEAEELNRAWAFAICRQRPFVTWKYAATL
                     DGRVAAADGTSRWITSPQARRDVHARRSRVNAVLVGTGTVLADDPWLTVRADDDTLAP
                     TQPLRVVMGRTPIPSTARVLDDAAPSLLLRTRDPHEAIAALHARGLRHVWLEGGPTLA
                     AAFVRAGLVDEVVAYLAPALLGAGPAALADAGVATIGHALRLALREVTQVGPDLRIIA
                     RPLAEGGH"
     gene            10361..>10694
                     /locus_tag="VLC50_07605"
     CDS             10361..>10694
                     /locus_tag="VLC50_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008406352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HSF27364.1"
                     /translation="MFTGIIEEVGEVLALTPAGDGARLSVRGPVVTAGVGHGASIAVD
                     GVCLTVARLDGDAFTADVMRETLDRTTLGGHGPGTRVNLERAVPAGGRLDGHVVQGHV
                     DGTGTVLAR"
BASE COUNT         1267 a         4031 c         4033 g         1363 t
ORIGIN      
        1 ggcgaccgcg gtggccaggg ccaggctgcc gggcagaccc gcccaccccg gcagggtgac
       61 ctcctcgtcg accgggacac gctcgtggct gaccgccgcg cggaccgccg ccgccgaggc
      121 cggatccagc ccggccagca gccgccccgc caaccccggc aacgccggtg gctcggccgg
      181 gacggccggc gcgggcgggt caaccggcag gagcgggtca gccggcgcga gcgggccagc
      241 cggcccgggc acttcggtgt ggctggtgtc ggtgggctcc gacccgatgg cgtcgtcggt
      301 cggaccatcc cccgtgttat cgaacatgcg ttcgagtgta gccgatagat gcgctaaacg
      361 gaagcacctg gacgtaatcc gggactacca gttgtatccg gccgtacgac agtgccaggc
      421 cggtcggtca gttctggtag accacgcgtc gtggacatcg cagcgcaggc cggcatcgcc
      481 cgcgtggccg tggacacccc gctgccccac ctcgaccggc tcttcgacta cctcgtcccc
      541 gccgagctcg acggggccgc cgcgcccggc gtgcgggtca gggtccgttt cgccggcaag
      601 ctcgtcgacg gcttcctgct cgagcgggtg gcgacgagcg agcactccgg tcggctcacc
      661 ccgctggcct cggtggtgtc cgaagagccg gtcctcgacc cggagaccct cgcgctgtcc
      721 cggctggtgg ccgctcgcta cgccggctcc ttggccgacg tcctccggct ggcagtgcca
      781 ccgcgccatg cccgggccga acgggcgccg gcacccgcgc cgccgaccga gccgccgccg
      841 ccgaccgtcg tggggccgtg ggatcggtat cggcacgggg tggggttcct cgacgccctg
      901 cgtgccgggt cggcgccccg cgcggtctgg acggcgctgc ccggcgaaga ctggccgacc
      961 gccctggcgg tggcggcgcg gtcaacgctc gacggcggcc gcggcaccct cgtcgtactc
     1021 cctgaccatc gtgacgtggc gcgcgtcgac ctggcgatgg gggcccagct cgggcccggc
     1081 cgtcacgtcg tactgaccgc cgagctcggc cccgaggcgc gctaccgacg ctggctgcag
     1141 ctgcgccgcg gccaggtcaa ggtggtcgtc ggcacccggg cggcggcgtt cgccccggtg
     1201 gccgacctgg gtctggtggc cctgtgggac gacggtgacg acctgcacgc cgagccgcgc
     1261 gcgccctacc cgcacgcccg cgaggtgctc atgctgcgcg cccaccacgg cgggaccgcg
     1321 gccctgctcg cgggttttgc ggtcaccgct gaggcgggcg cgatggtcga gagtggatgg
     1381 gcccgtccgg tcgtcgccgc ccgcgaggcg gtccgggcgg ccgcgccccg catcctcgcg
     1441 gccggcggtg acgacgagct ggcccgtgac ccggccgccg cggcggcccg gctgccgagc
     1501 ctggcctggc gcaccgctcg ggcggcgctg gccaccggac cggtgctcgt gcaggtcccg
     1561 cgccgcggct acctgccggc actggcctgc cagacgtgcc gcagcccggc ccggtgcccg
     1621 agctgccagg gtgcgctggc gatggcccag ggcagaccgg cgccggtgtg ccagcggtgc
     1681 gggcgggtcg agccggcctg gagatgcgcc gactgcggcg gacaacgctt gcgagccgcc
     1741 gtcatcggcg cgcgacgcac cgccgaggag ctcggccggg ccttcccctc ggtgccggtc
     1801 cggacctcgg ggcgtgacgg ggtgctggcc cgcgtcgacc cggttcccgc gctcgtggtg
     1861 tccacgccgg gcgccgagcc gctggtcgag ggcggctacg ccgcggccct gctgctcgac
     1921 gcctgggcac tgctcggccg ggccgacctg cgtgccggtg aggaggcgct gcggcggtgg
     1981 ttgacggccg ccgcgctggt tcgaccggca tcctcgggcg gccaggtcgt ggtgctggcc
     2041 gacgcgggac tggccccggt gcaggccctg gtgcggtggg acccggccgg cttcgcctcg
     2101 cgcgagctgg ccgagcggac cgcgctgcgg ctgccgccgg cggtgcgggt ggccgagctg
     2161 accgggccgg ccccagacgt cgaggcgatg ctcgcggctg tggcgctgcc gcccgcggcg
     2221 gaggtgctcg ggccggtccc cgccgaggag tccacggtgc gggtgctcgt gcgcgtggac
     2281 aagcgcgacg ggctggccct tgcgagcgcg ctgcacgcgg cggccgggtc ccgcagcgcg
     2341 cgcaagcagg gcgcgcacgt gcgaatccgc atcgatcctg ccgatctcgg atgatcccga
     2401 ccttgggcat ttcacctgac gtggcgcccg gcacgcctta ccgtcgacta cgagccggtc
     2461 cgccggaggt gggggatgag cgtcgaagcg acgagggacg acctgcacac ggtgcccgcg
     2521 gcgtcatcgc cttcagacgc cccgcccgcc gtgcccagac cgcgcaagcc tgaacagccg
     2581 gtagtgatcg ttgacgcggt cagtgactcc gcgatacgcc acagctgcgg ggccatggtc
     2641 acccagctgt tctcggtcgg cggcccgagc acctgcccgc tctgccacga gaccgtgtga
     2701 gccagcagcc acctagactg ggtggcgggt cctgctggcg agcgacgagg agaaccgtgg
     2761 cgatcaagcc catccgcctt ttcggcgatc ccgtgctgcg caccccggcg gcaccggttg
     2821 tcgacttcga caaggagctg cgccagctcg tcgccgacct gaccgacacg atgcgcgatg
     2881 ccccgggggc cgggctggcc gctccccagc tcggcgtcag cctgcgggtg ttcacctact
     2941 gggtcgatga cgagctcggc cacctggtca accccagcct cgacctctcc gacgaggagc
     3001 aggacggcga agagggctgc ctgtccttcc ccggccttta cttcgacacc aagcgggcgc
     3061 tgcgggtggt ggccaagggc caggacatgt acggcgagcc cgtggtcatc gagggcagcg
     3121 acctgctcgc gcgctgcgtg cagcacgaga ccgaccacct cgacggcgtc ctgttcatcg
     3181 accggctcga ccgcgagacc cgcaagctcg cgctcaaggc gatccgcgag gccgagtggt
     3241 ctgagggggc gcccccggtg gtcaagacct ccccgcaccc gacgttcggc aaggcgatct
     3301 agccggtgcg cctggtcttc gcgggcaccc ccgaggtggc ggtgccctcc cttgacgcgc
     3361 tcctggcctc gcgccacgac gtggtggctg tgctcacccg ccccgacgcg cccgccggac
     3421 gcggccgcac ggtcgtggcc tcgccggtcg cgcgccgggc cgaggcggct gagatcccgg
     3481 tgctcaagcc cacccggctg cgcgacgagg ccttcctcga cacgctgcgc gcgctggccc
     3541 ccgacgccgc cccggtggtg gcctacggcg cgctggtccc tccggccgcc ctgacgatcc
     3601 cgaggcacgg ctgggtcaac ctgcacttct cgctgctgcc ggcctggcgc ggcgccgccc
     3661 cggtgcctca cgcgctgctg gccggcgacg aggtcaccgg tgcgacgacg ttccggctcg
     3721 acgaggggct ggacaccggc ccgacctacg ggtgcctcac cgaggccgtc ggcgctcacg
     3781 acaccgccgg cgacctgctc gaccggctgg ccgaggccgg cgccgcgctg ctcgtggcca
     3841 ccctcgacgc gcttgaggac ggcaccatcg aggcccgtga ccagccggtc gagggggtct
     3901 cgttcgctcc caaggtcacc gtggagcagg cgcgggtcga ctggtccgcg ccggcgttcg
     3961 cggtcgaccg gctggtccgg gccatgaccc cgcacccggg ggcctggacc acctggcgcg
     4021 gccagaggct ggggctgggc ccgctcgagc ccgccgacga cgccgggctg gccccgggcg
     4081 acgtgcgggt gacccgtcgc gaggtgctcg tcggcaccgg cacggccgcc gtacgcctgc
     4141 acgaggtgcg tcccgccggc cggcgcccga tgcccgcggt cgactgggcc cgcggggccc
     4201 ggccgcagcc gggggagcgg ctcgggaacc caaccgagga cgaggtcgat gcccgctagc
     4261 gcccgcactc gccggagccg accggcgcgc accgacccgg cccgccgggt ggcctacgac
     4321 ctgctgcgtg cggtcgacga gcacgacagc tacgccaacc tggccctgcc ggccctgctg
     4381 cgccagcgtg ggctgcacgg gcgtgacgcg gcgctggcca ccgagctcgc ctacggcacc
     4441 ctgcgtggcc gaggtaccta cgacgccgtg ctggccgcgt gcgtggaccg cccgctcgac
     4501 cagctcgacc cgcccgtcct cgatgtgctc cggctcggtg cccaccagct gctcgagacc
     4561 cgcatccccc cgcacgccgc ggtcgccagc accgtcgcgc tggccaggga ggccctcacg
     4621 gccggcccgg ccgggctggt caacgccgtg ctgcgccgcg tcgccgagca cgacctgccg
     4681 acctggttgg cccggctcgc cccagacgcc gaggccgacc ccgacggcca cctcgcggtg
     4741 accaccagcc accctgcctg ggtggtgcgg gcgctgcggg atgcgctgcg cgccgacggg
     4801 cgcgacccgg ccgagctggc tcgcctgctc gcggccgaca accgtgcacc ggcggtgaca
     4861 ctggtcgccc ggcccggccg ggccagccgc gacgagctcg tggcggccgg cgcgaatccc
     4921 gggcgctggt cgccgtacgc cgccgtcctg ccggccggcg acccggcggg agtccccgcc
     4981 gtcgcggcgg gccgggccgg ggtgcaggac gagggcagtc agctcgtcgc gctcatgctg
     5041 cgcgccgccc cgctcgccgg ccccgacacc cggtgggccg acctgtgcgc cgggccgggc
     5101 ggcaaggccg cgctgctcgc cgcgctcgcc gacccggtcg gcgccgagct ggtcgcggtc
     5161 gagtcggccg cccaccgggc cgacctcgtg cgccgagcgc tgaccggcac ccccggccgg
     5221 tggcgggtcg aggtgggcga cggccgggcc gtgggtgccg cggtgaccga tggagcggtc
     5281 cagccctacg accgggtcct ggtcgacgcg ccgtgcacgg ggctgggcgc cctgcgccgt
     5341 cgccccgagg ctcgctggcg caagacgccg gccgacgtcg cgacgctgac tcggcttcag
     5401 cgggagctgc tcgggtcggc gctcgacgcg gtccgtcccg gcggggtggt ggcctacgcc
     5461 acctgctcgc cgcacgtggc cgagacccgg gtcgtcgtcg acgacgtgct gcgccgtcgc
     5521 ggcgacgtgg aggccctcga cgcgggcgcc gtcttgcgcg acgtggcacc cgatctgccg
     5581 ctaggtcccg ggccctacgc ccagctgtgg ccgcacgtgc accgcacgga cgcgatgttc
     5641 gtggcgctcc tgacccgccg ataggctcgc cgccgtgggc gtgcagatct ctcccagcat
     5701 cctctcggcc gacttcgcca acctcgagcg tgagctcgcc cgggtggcca acgccgactg
     5761 ggtccatgtc gacgtcatgg acaaccactt cgtgcccaac ctcacgctcg gcctgcccgt
     5821 ggtcgagcgg ctggtcgcgg tgagcccgcg tccggtcgac gtgcacctga tgatcgacga
     5881 tcccgaccgc tgggcccctg cctacgccga ggcgggcgcg cacagcgtga cgttccacgt
     5941 cgaggcggcg gcggcaccgg tgcggctggc ccgcgagctg cgggcggccg gcagccgggc
     6001 ggcgatggcg ctgcggccgg cgacgccggt cgagccctac gccgacctgc tgcccgagct
     6061 cgacatgctg ctgctcatga cagtcgagcc gggtttcggt ggccagccgt tcctcgacgt
     6121 cgtgctcccc aagatccgcc gggcgcgggc gatcatcgag cgcgccggca tcgaggtgtg
     6181 gctacaggtc gacggcgggg tgtccagcga gaccatcgag cgggccgcgg ccgccggggc
     6241 cgacgtcttc gtcgccggct cggcggtcta ccgggccgag gacgccgcgg cggcggtcga
     6301 cgagcttcgg gccctcgccg agaaggcggc cggcgccccg ccggcctgac gcctcacgac
     6361 gtccaggacg acgtcaggac gacgtaagga cggcgtcagg acggcgtcag gacggcgtca
     6421 ggacgacgtc aggaccagcg cagaggccgc gggggcggtg ccagctgggc ccgggggcac
     6481 gacgagagcg tgaggtcggc gtggcggaac gcgtcgtcgc acaagcgctc ggtggtccag
     6541 ccgtggcgca ggccgtagga cgtcagcgac acccggaaca ccgtgaccgc gtagcgggtc
     6601 gggctgtcat agacgcagct gatcgagccc aggtggactg cctcgcccgg ctcgtcgctg
     6661 cccagctccc acggcttgtc gtagcggcgc cagaccccga cgcgggtcca ccagtgcccg
     6721 ctgttgcgca cgtcgtcgcg ggcgagcccg gccaggatcc gccgggcgtc ggcctcggag
     6781 agcacggccg cggggcggat gacctcggtc gaggcgaagt cgtcggagcg gccggccccg
     6841 tgcggccctc caccggtacg gcgggtcggc tgcgcggaca tcgtcaggga ggcgggggtc
     6901 atcgtgggtc cttccctagg gacggtaggg gcggtagggg ctgcgggcgg ccgaacagga
     6961 agccctgggc gtgcgagcag ccgagctcgc gcagccggtc ggcctgggcc tcggtctcca
     7021 cgccctcggc caccgtcggc agcccgaggt cgtggcacat gctcagcacg gcccgcacga
     7081 tcgcgtgggc gctgcgatcg tgcagcagcg gctggacgaa gctgcggtcg atcttgacca
     7141 tgtcgaccgg gaagtcgcgc agcaggctca gcgaggagta gccggtgccg aagtcgtcga
     7201 gcgcgatgcg cacacccatg cccgagatct cgcgcagctc gtcggccagc gcgccgctgg
     7261 cctggatcag cgcggtctcg gtgacctcca ggtgcagctg ttcgggacgt acgccggact
     7321 cctcgagcgt gcgccgtacg acgccggcca ctcccgggcg ggagagctgg ctcggcgagg
     7381 cgttgaccga cacccaggtc ccgttcgcgc acagccgcgc gccgggcgcg gtggcgtcgg
     7441 agcagacccg tgcgagcatc caccagccga tcgagctgat cagcccgctg ctctcggcca
     7501 ggtcgaggaa cacctcgggc gcgagcagcc cgcgggccgg gtgctgccag cgcagcagcg
     7561 actcgtagcc caccaggcgc tcctcgcgca ggctgacgat cggctggtag tgcgcgcgca
     7621 gctcgccggc ctcgacggac tcgcgcaggt tgtcctcgag ctcgaggtcg tcgcgggtcg
     7681 cgatccggtc ccgctccacg tcgaagagct cgatgcgggc ccggcccagc cgcttggccc
     7741 gcagcagcgc catgtcggcg cgcaccagca ggtcctcggc ggtctccggg tcctgcccga
     7801 tcgccgcgcc gatgctcagc gtcacggtgt gcgggtgccc acccacccgc accggctcgg
     7861 tcagcgcgcg gaccagccgg gcggccacgc cgagcaggtc gtcgggtccg tcgagccgct
     7921 ccgccacgac gatgaactcg tcgccgccca cccggcccac caggtcgtcg tcgcgcagcg
     7981 cgtcacgcag ccgctgggac acctcgacga gcagctcgtc gccgcaggcg tggccgagcg
     8041 agtcgttgac cagcttgaac cggtccaggt cgcagtagag caccccgacc ggctggccgg
     8101 ggtggtcgcc caacgcgctg gccagccact gacgggtcat gaagcggttg ggcagcccgg
     8161 tcagcgcgtc gtgcagcgcc tggctggcca gcgccagctc cgcgtcgcgc cgttcggtga
     8221 tgtcctgcag ctggatcatc acggcgtgca ggtcgccgtc gtcgtcgagc accggggccg
     8281 cggtgaggtg gcaccagagc atccggccgt tcgcggtgag gtagcggtgg tcggcggcgt
     8341 aggtggccct ccggccctcg agcagctcct gccagtgcag ccacatcgct gaccagtcct
     8401 cgggcgcggt gacgctgcgc accgtggagc gggtcagctc ctcggcccgg tagtcgagca
     8461 tggcggccag cgcgtcgttg accagcagga agccgccgtc aggggcgacc agcgccccac
     8521 cgatcgggga gtgctcgaag gccaggcgcc attggtgctc ggaggccgcc aggtgggtca
     8581 tcgcgagaac gtcgtcggtg acgtcacggg cgctgagata ggcgtaggtg ccgtctggct
     8641 cggtggcgaa cagcttgccg gcgacgtcga gccagaccgt gccgccgtcg gcaccgtcga
     8701 gccggatcgt gctgcgcagg ctgcccgcct cgccgagccg gctgccgaag gcgcgcagca
     8761 ccggcaggtc gtgctcggtc gcgcaggcgg cgatgccctc ccgctgcaac agctcgaggt
     8821 cccacccgag cacggtctcg acggtcgtgc tggcgctgag cacgtgcccg tcgagcctca
     8881 tggcgagcac caggtcggcg tcggtgtgcg gcacgtccgc ggcgtcactg accgagcggt
     8941 cggttgccgt tgtcatgatc ctccccgtgg ttcccccacc tagatcggcg ggcgggccat
     9001 cgacctgaac agaccgccgg cccgagcggg cgcctcgccc gcgcccgggc ggccgtggca
     9061 gactgagcgc gaacacactg cgtgctccgg ggtcggtgaa agtccgaacc ggcggtgaca
     9121 gtccgcgacc cgaccgaagc catcggccgg ttgaccaggt gcaattcctg gaccgacggt
     9181 gagagtccgg atgggaggca gcacgcacgg ctgggcccga gcggggctca gccgcaggcg
     9241 tcgtacggcg ccgggtcggc caccgcaggt ggcgccggtg ccccagccgc ccggcccgga
     9301 gcgcgcccga ggaagggagc gcgggcgccg tggccaccac cgccgagctg gctgcgatgc
     9361 gtcgcgccct cgagctggcc gagatccccg gcggaccacc ggcgaccaac ccgcgggtcg
     9421 gcgccgtcct tctctctccc gacgggcggg tcctcggcga ggggtggcat cgcggcgccg
     9481 gcaccccgca tgccgaggtc gaggccctcg ccgccgccgg cgagtcggcc cgcggggcga
     9541 ccgctgtcgt cacgctcgag ccgtgcgacc acgtggggcg caccgggccg tgcagccagg
     9601 cactcatcga cgccggcatc gcccgggtgg tctacgcgat ggccgacccc aacccggtgg
     9661 ccggcggcgg tgccgggcgg ttgcgcgcgg ccggcgtcga ggtcgaggcc ggtccgctgg
     9721 ccgaggaggc cgaggagctc aaccgggcct gggcgttcgc gatctgccgc cagcgtccgt
     9781 tcgtgacctg gaagtacgcc gccaccctcg acgggcgggt ggccgccgcc gacggcacga
     9841 gcaggtggat cacctcgccg caggcccgcc gcgacgtgca cgcgcggcgg agccgggtca
     9901 acgcggttct cgtgggcacc ggcaccgtgc tggccgacga cccctggctg accgtgcgcg
     9961 ccgacgacga cacgctggcc ccgacgcagc cgctgcgcgt cgtgatgggc cgcacgccca
    10021 tcccgtcgac cgcgcgggtg ctcgacgacg cggcgcccag cctgctgctg cgcacccgcg
    10081 acccgcacga ggcgatcgcg gccctgcacg cccgcggcct gcgccacgtc tggctcgagg
    10141 ggggtccgac gctggccgcc gccttcgtgc gggccggcct ggtcgacgag gtggtcgcct
    10201 acctcgcgcc cgccctgctc ggcgccggcc cggccgcgct cgccgacgcc ggtgtggcga
    10261 ccatcgggca cgcactgcga ctggcgctgc gtgaggtcac gcaggtcgga cccgacctgc
    10321 gcatcatcgc caggccgttg gctgagggag gccactagag gtgttcacgg ggatcatcga
    10381 agaagtcggc gaggtgctcg ccctgacccc tgccggcgac ggtgcccggc tgtcggtgcg
    10441 cggcccggtc gtcacggcgg gcgtcgggca cggtgcctcg atcgccgtcg acggcgtgtg
    10501 tctgaccgtc gcgcggctgg acggagacgc cttcacggcc gacgtcatgc gcgagacgct
    10561 cgaccgcacc acgctcggcg ggcacgggcc gggcacgcgg gtcaacctcg agcgggcggt
    10621 tcctgccggc gggcgcctcg acgggcacgt cgtgcagggg cacgtcgacg gcacgggcac
    10681 cgtgctcgcc cggg
//
LOCUS       DATIBB010000192        13956 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_126666, whole genome shotgun sequence.
ACCESSION   DATIBB010000192 DATIBB010000000
VERSION     DATIBB010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 13956)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13956)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13956
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126666"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..406)
                     /locus_tag="VLC50_07610"
     CDS             complement(<1..406)
                     /locus_tag="VLC50_07610"
                     /inference="COORDINATES: protein motif:HMM:NF025736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HSF27365.1"
                     /translation="MTAFDATAAARRLGVALRRAPLWAEVAVIVAFYVGYAVVQGAAP
                     TDAESASAHGLAIWQLEQEVGLAPEHWLNQMAVQHAWVAQISGWFYGTAHFVLTPIVL
                     GWLYLCRSRVYGQWRTTLAVASLIALAVYWVYP"
     gene            588..1340
                     /locus_tag="VLC50_07615"
     CDS             588..1340
                     /locus_tag="VLC50_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015580129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HSF27366.1"
                     /translation="MDAVFKALADRTRRALLDELFREDGQPLRALEERFAMSRFGVMK
                     HLKTLEDAGLVVARRRGREKLHFLNPVPIRLIHDRWVSKYAEPWVAGLSDLKHRLEET
                     MEKVFEIYIRTTPERLWEAITDGETRSKYNFGARVSSDWTPGSRFEMSHPGGGLLGEG
                     DILEADPPRRLVQTMVALWSDEVKSEGASRVTWEIEPVGEDSCRLVVTHDQLREGANE
                     ELYGGWPMILSGLKTWLETGQQLTTPGSLLYG"
     gene            complement(1331..2185)
                     /locus_tag="VLC50_07620"
     CDS             complement(1331..2185)
                     /locus_tag="VLC50_07620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014145736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HSF27367.1"
                     /translation="MSMPMQVVKQLEGLEGLDPLIKQLQHVVSRLVKPRIVRNTLSGT
                     TLGHALHPMLTDLPIGAWTAAGLLDLVGGKRSTHAADLLVGAGIAAAVPTAAAGANDW
                     SDTYGDEARVGLVHAALNSTALLLYGASLAARASRHRTLGRLLGWSGLAAVVASGYLG
                     GHLTFAQGVNVNHTAFEERPTDWTPATSSAELGEGATIAATAGSATVFLHRHDGRIYA
                     LANTCSHLGGPLNEGTVHDGCITCPWHGSTFRLDTGKVVRGPARTAQPTYDVREAAGQ
                     MEVRAAQP"
     gene            complement(2286..2681)
                     /locus_tag="VLC50_07625"
     CDS             complement(2286..2681)
                     /locus_tag="VLC50_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017241752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HSF27368.1"
                     /translation="MPLGPQTHIRIARPSRDLAAAERFWVDGLGLQVLWRSEPEGPAE
                     HALLMVGWPNASWHLELVADPSTLPAPTEEDLLVLYVGDPIDDALVRRLLAHGGARVQ
                     PRNPYWDRWGVSIVDPDGYCLVLSHRSWG"
     gene            complement(2687..2950)
                     /locus_tag="VLC50_07630"
     CDS             complement(2687..2950)
                     /locus_tag="VLC50_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020637830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ada metal-binding domain-containing protein"
                     /protein_id="HSF27369.1"
                     /translation="MTYTLLGPDGRPYESIVPGTLGGHRRGRIYGRLDCPSALRAIAR
                     GGYVTHRVFFADEATAVAAGFRPCARCMPDEYAAWKAKQPVRS"
     gene            complement(2947..4833)
                     /locus_tag="VLC50_07635"
     CDS             complement(2947..4833)
                     /locus_tag="VLC50_07635"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HSF27370.1"
                     /translation="MKPAVALGLRLALGTTRADRLRSAMVALSAAIGTLLLLVVAAIA
                     RAEQAQHPNLYSSVEMQRLLLAVVLAVALPVLVLAATAGRLSAALRDRRLANLRLLGL
                     SPSRTRAVAVVEAGAAAVAGTAVGLVAFTVARPVLARIDVARRHWAAETLHPHVLDEF
                     AVIIVLPVVVVALAALPHRLDTSSAMRAARKADAKRPSLWRLPPLVAGLALCAVVIAR
                     SGQGDPGDDLILSLFAGVGLLGLGTILVVPIFMRVVADLLLRVGGGPATTIAARRLQA
                     QPASVTRVVAGLMIGLFLVTGARWVVGNFEALPQYVGAKRALTVQQQVAVLTTPADRA
                     STQARAEAVAGVRRTVAFSSLMSGCTNTSENCLFALVGTCDDLHALAPELTGCRSDQA
                     TWLRPSVADRLDRASAIRWSPSPESDVSLSLPIPAESISGMRDGLLRPLNEGQVFIPR
                     GLPGLDRVLEHSSLDVVAIGYAGQDLPDRLLAAGLNASGGEDFDTYDFVAGLRAMVWA
                     IAAVILSVGLLAFAVAAIDRAVSRRREVVSLQLVGVSPRVLRRTQWIEAALPIVTGSV
                     LAIGTGLLAGATYLSLVEDLDHAPWRQSLGLVAVAANSACIIAGLTVLAASPRIRPDL
                     IRTE"
     gene            complement(4830..5516)
                     /locus_tag="VLC50_07640"
     CDS             complement(4830..5516)
                     /locus_tag="VLC50_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011604798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HSF27371.1"
                     /translation="MSGSSVLEAREVGLSYGDTVALRGVNLSLATGERVALMGPSGSG
                     KSSLLHCLAGIIRPTGGEVAVEGTRLDTMTESARSALRLQRIGVVFQFGDLVPELTLA
                     ENVMLPLQLLGRRRAQSRSAAMDLLAALDVDSVANKRAGTVSGGQAQRAAVARALVHQ
                     PAIVLADEPTGALDSLSAETVMDALVSLTTAIGAALLVVTHDNLVAAHLDRHLVMRDG
                     AISDTVGAGR"
     gene            complement(5513..6043)
                     /locus_tag="VLC50_07645"
     CDS             complement(5513..6043)
                     /locus_tag="VLC50_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020574529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HSF27372.1"
                     /translation="MTTAHALLALLEREPAHGYTLKQQYDARFARIRPLAFGQVYASL
                     ARFERQGWARLHDVEAGDGPDRKRYAITPAGTDVVDAWVFEPQPPGAFATSNLLARVS
                     VALISGRSAETVLATQRATHLGRMRELTRQRKNADPAEVLAISYELAHLDADLRWIEE
                     SGQRLDQAREAAAGRG"
     gene            6142..6495
                     /locus_tag="VLC50_07650"
     CDS             6142..6495
                     /locus_tag="VLC50_07650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF27373.1"
                     /translation="MTRAQGWYYLLGGIWPLVHFRSFQLVAGPKPDRFQTEVTSGLFV
                     AIGAALLSSPRDPPPPRTALVLSAASGLATAYVDLRHRRDIRGLFDVEAAVEAGFALV
                     ALRSLLRARRGHGTH"
     gene            6622..9828
                     /locus_tag="VLC50_07655"
     CDS             6622..9828
                     /locus_tag="VLC50_07655"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSF27374.1"
                     /translation="MTRVLARRARAHSLLPVTAVMTVLLAATGLATLLTYSAVVPQQG
                     LVRLLGSDTARAAVFVDAALGQDFAARDEIVREAVGHRLGAVPTRVDVAGQSAPYRLP
                     PEVGAPGVLTSFWFLDDLAGQARLVSGRWPAPEPAGSPVEVALSQPAAQRMALEPGRR
                     LSLTSATFAPAVDVVVTGVYEPVDPAGPVVRLDPTGGAGVTEGEFTSLGPLAVAPDVF
                     MARFAASARVSWLAQPVLTGLDPAGLAPLQGDMAALRADLSAASVFNGAVQVRTGLDG
                     LMTQALRSLVVSRSTLPVPLLLLAVLAFCAIAYVGSLLHERRRGQRDLLRARGASRSQ
                     LVTAALLEGLAIAGPPALVAPFLATGLLRWASNPLGIGDGAGLSVALSRGTWAWMVAV
                     ALAATLTLAAPALVPAGTGGRGRWRGRVRPGVERAGVDIALLLVAGAGYLQLRAAGSP
                     FAAADLREVRADPVFVLAPVILLVAVALLGLRLLPVLARLAGHLAGAGRSASPALGAW
                     PVARRPRRYATSLLLLVLALSVGLMSAVATASGQRSRADQANHQVGSDVRVELETTRA
                     ADQDGVARLATLPGVAATLPAAREQVDTGPGPDTTLLALDAGQASDVMLLRADLLDRP
                     TEDAFAAMRAPLDEAAVALPDDARALRGQVSLTVVAGADEQPDALLAGSVSIVVRDRA
                     DLVHVVPWAEPSNDGAPTRVTAALPAGSTALLGFVFGYTVPPRLIAQGPSDGHASVRI
                     SGLELDDGRPVRMGTTEWSTAVDHSGARSWAPVQVSAGDALEVAFGIGQPSAQVPVLA
                     GAPEQLDRPITLTAIGSQQLLRSHDVAVGDLVGARIRGVTTQVRLAAAAVDVPTVSSS
                     EALVVDLPTVQRLLLRESGRTIAPSEAWLRAEPGQTQAVAAAIRDQPGAAVAVVDRES
                     VRAALDEDPLSVAFQGALRIALVGGVLFAVVGFVVSALLSVDERRDELAVLRALGMRP
                     SDLSKTMLVEQALVLGLGTVLGLVVGLVVSLLVVPLTTVSSRTGAVMPPISLQVPWVQ
                     AATLAMALAAVMLVVVVGGVTTLVRGRLGPTLRTRQAP"
     gene            9825..11972
                     /locus_tag="VLC50_07660"
     CDS             9825..11972
                     /locus_tag="VLC50_07660"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HSF27375.1"
                     /translation="MTSRIVIRRLRRDGRGLPLFYAVLVVLVAALSVAAPLVLRSAAG
                     QALRDEVTRADDQVRTITVRQSVSFRGARLEGHARPAVAATRVKLRQGMAPSLRGVVG
                     TGTYAAQSTPYDVLDARTGARPDPTSHQKLLLRAQSEALERVQWVRGAAPVGYDLTEV
                     EGRSTPRVQVALETTVAAALHVDVDDVLSLTPERFSQGMTVRVTGVFAVERPDRWFWD
                     GEPRVLVPAVKDDGNTRVITGAALFDPGAIEVVDALAFQSLDVAYRFPVTIAALDTVS
                     APQAVTALGAYQSWVRQNAGPGFDASSRLPTAVEQFTSEMSTTNALLSVALVGLGAGV
                     ATATALVAWLLVRRRSRTLELLRARGASLRQVALVVPRECAPVALALALSGSVLAVAA
                     LGREAARPIATGWALTLIGLALPAGIAASRHRRVVGHDAASAGAGRRVTLELTVAALT
                     LGGLALLLRAGLRASGMTAVRAVVPTLLAVLIGLLVLRGASSVLTPLRRRASAGRSVV
                     GLLVLTGMGRHRGASVVPVLVLVVTLAMATFGLVTTSTLLAHRPADAPASTAAALGAG
                     PTGESPMVAILLTGLGLAGLGSAVCALLVLVVAQLGSRRSRAGDQSLVRTLGFSQGDS
                     RRLVTLSLVPQAVVALVAGVLAGLAVSALVVATVDLAPFTGAEGTALAIEAGWLAALV
                     GAFLLLAAVTVLAEAAADRHRPIASDLRAGVPS"
     gene            11969..12961
                     /locus_tag="VLC50_07665"
     CDS             11969..12961
                     /locus_tag="VLC50_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015577183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSF27376.1"
                     /translation="MSRSTPSRSELIDLPLPTTGRSTYGADAQVVCDNVVRIYRSAGV
                     EVVALQGLDLLVDRGELVAIIGPSGSGKSTLLRILSGLDVPTAGAVRVAGHDLGAMGR
                     RELLAYRRRVVGFVWQQTGRNLLPYLSAHENVALPLGIAGRPAREAAARADELLELLE
                     VAHRRDQRPTEMSGGEQQRAAIAVALANSPEVLFADEPTGELDSATAADVFAALRHAN
                     SELGVTVIVVTHDALVSSQVQRSISIRDGRTSTETLRRVDAPVDASGRVVAQEFAVLD
                     RAGRLQLPAEFTAALDLRRRVRLELERDHIGVWPDAPAAETDGTHDDRGVGNHA"
     gene            12954..13679
                     /locus_tag="VLC50_07670"
     CDS             12954..13679
                     /locus_tag="VLC50_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018788489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSF27377.1"
                     /translation="MPEPMLLAEGLTRTYGEGSRAVHALRGVDLRVETGELVAIRGRS
                     GSGKTTLLNLIGGLDRPDAGTIVVDGHEVTGLDEDGLRALRRETVGYVFQTFALIPTL
                     TAAENVGIPLRLRRVAPAAREARVAQLLDLVGLADHGAQTPEQLSGGQQQRVALARAL
                     AGQPRLLLADEPTGQLDSHTGREVMALLSAVVHEAGATALVATHDPGVLAVADRVLEI
                     RDGTLTPDLLAAQALAREQAMGG"
BASE COUNT         1833 a         4865 c         5147 g         2111 t
ORIGIN      
        1 ccggatagac ccagtagacg gcgagcgcga tcaaggaggc caccgcgagc gtcgtacgcc
       61 actggccgta gacccgtgac cggcagaggt agagccagcc cagcacgatc ggcgtgagca
      121 cgaagtgcgc ggtcccgtag aaccagccgg agatctgggc cacccaggcg tgctggacgg
      181 ccatctggtt gagccagtgc tccggcgcca gcccgacctc ttgctcgagc tgccagatcg
      241 ccaggccatg cgcgctggcc gactcggcat cggtcggtgc ggccccctgc accaccgcgt
      301 agccgacgta gaaggcgacg atgacggcca cctcggccca caacggggca cggcgcaggg
      361 ccacgcccag acggcgcgcg gcggcggtgg cgtcaaacgc tgtcacgacg ctgaagctcc
      421 aggcactacc gctccccacc ggctcgatga cgttggtaca cccagcggct ggcccgcctc
      481 gccttgccaa tccttgcatg cgccgtggtc ctggatgcgt caagtgactt gccgacgcgc
      541 cgccgggggt cttgacaggt gaccaaatgg tcacctaact ttcagaggtg gatgcggtgt
      601 tcaaggccct cgccgaccgc acgaggcggg cactgctcga cgagctcttt cgcgaggacg
      661 ggcagccgct gcgcgcgctc gaggagcggt ttgcgatgag ccgcttcggg gtgatgaagc
      721 acctcaagac gctcgaggac gcgggcctgg tcgtcgccag gcgccgaggc cgcgagaagc
      781 tgcacttcct gaacccggtc ccgatccggc tcatccacga ccggtgggtg agcaagtacg
      841 ccgagccctg ggtcgctggt ctcagcgacc tcaagcacag gctggaggag accatggaga
      901 aggtcttcga gatctacatc cggaccacgc cggagcgcct ctgggaggcg atcaccgacg
      961 gggagacccg cagcaagtac aacttcggtg cccgggtcag ctcggactgg acgcccggct
     1021 cgcgtttcga gatgagccat ccgggcggtg ggctgctcgg tgagggcgac atcctcgagg
     1081 ccgacccgcc acgccgcctg gtccagacca tggtcgccct gtggagcgac gaggtgaaga
     1141 gcgagggggc ctcccgcgtc acctgggaga tcgaaccggt cggcgaggac tcctgccggc
     1201 tcgtcgtgac ccatgaccag ctgcgcgagg gtgccaacga ggagctctac ggcggttggc
     1261 cgatgatcct ctccggcctg aagacctggc tggagaccgg tcagcagctg accacgccgg
     1321 ggtcgctgct ctacggctga gccgcgcgca cctccatctg gccggcggct tcgcggacgt
     1381 cgtacgtcgg ctgcgcggtt cgggccggtc cccgcacgac cttgccggtg tcgaggcgga
     1441 acgtgctgcc gtgccagggg caggtgatgc agccgtcgtg cacggtcccc tcgttgagcg
     1501 ggccgccgag gtgggagcac gtgttggcga gcgcgtagat gcgtccgtcg tggcggtgca
     1561 ggaaaaccgt tgcggaccct gccgttgcgg cgatggtcgc gccctcgccg agctcggcac
     1621 tgctggtcgc tggtgtccag tcggtgggcc gctcctcgaa ggcggtgtgg ttgacattga
     1681 cgccctgggc aaaggtcagg tgcccgccga ggtagccgct ggccacgacg gcggccagcc
     1741 ccgaccagcc gagcagccgc cccagcgtcc ggtgccggga cgcacgggcg gccagcgacg
     1801 cgccgtacag cagcagggcg gtgctgttga gggcggcatg gaccagcccc accctggcct
     1861 cgtcgccgta ggtgtctgac cagtcgttgg ccccggccgc tgcggtcggc acggcggcgg
     1921 cgatgcccgc gccgaccagc aggtcggcgg catgcgtgct gcgtttgccg ccgaccaggt
     1981 cgagcaggcc ggccgcggtc cacgcgccga tcggcaggtc ggtgagcatg gggtgcagcg
     2041 cgtggccaag agttgttccg ctcagggtgt tgcggacgat ccgaggcttc acgaggcgcg
     2101 agacgacgtg ctggagctgc ttgatcagcg ggtcgagccc ttcgagacct tcgagctgct
     2161 tgacgacctg catgggcatg ctcatgtggc gcctccccat ggccgacgag gcggcgggtg
     2221 cacgccccgg tgtggtcccc cgccatgatg cgccgacgat cgccagcttg ggacccgatg
     2281 ccgctctagc cccaggagcg gtggctgagg acgaggcagt agccgtcggg gtcgacgatc
     2341 gagacgcccc accggtccca gtagggattg cgtggctgga cgcgagctcc tccgtgcgcg
     2401 agcaaccgtc gcacgagggc gtcgtcgatc gggtcgccga cgtagagcac gagcaggtct
     2461 tcctccgtgg gagctggcag cgtgctcgga tcggcgacca gctcaaggtg ccagctcgcg
     2521 ttcggccaac ccaccatcag cagcgcgtgc tccgctggtc cctcaggttc gcttctccat
     2581 aggacctgca ggcctagacc gtcgacccag aatcgctctg cagcggccag gtcgcggctg
     2641 ggacgggcga tgcggatgtg tgtttgtggg ccgagcggca tgcccgtcat gatcgcaccg
     2701 gctgtttcgc cttccatgcg gcgtactcgt cgggcatgca gcgggcgcac ggccggaacc
     2761 ccgccgcgac cgcggtggcc tcgtcggcga agaacactcg gtgggtgacg taaccgccgc
     2821 gggcgatcgc gcgcagggcg ctcgggcagt cgagccggcc atagatgcgt ccgcgccggt
     2881 ggccgcccag cgtgcccggc acaatgctct cgtagggcct tccgtccggc cccagcagtg
     2941 tgtaggtcat tcggtgcgga tcaggtcagg tcggatgcgt gggctcgcag cgagcaccgt
     3001 caggccggcg atgatgcacg ctgagttggc ggccacggcg acgaggccca gcgactgccg
     3061 ccacggcgcg tggtccaggt cctcaaccag cgagaggtag gtcgccccag ccagaagccc
     3121 ggtgccgatg gcgagcactg aaccggtgac gattggcagg gcggcctcga tccactgcgt
     3181 acgacgaagc acgcgcggcg agaccccgac cagctgcagg gagaccacct cccggcggcg
     3241 agagaccgcg cggtcgatcg ccgcgaccgc gaaggccagc agcccgacac tgagaatgac
     3301 cgcggcgatc gcccacacca tcgcccgcag ccccgcgaca aagtcgtagg tgtcgaagtc
     3361 ctcgcctccc gaggcgttga gcccggccgc gagaagtctg tcggggaggt cctggccagc
     3421 ataaccgatg gcgacgacgt ccaacgagct gtgctccagg accctgtcga gaccgggaag
     3481 gccgcgaggt atgaacacct ggccctcgtt gagaggtcga aggagaccgt cgcgcattcc
     3541 gctgatgctt tccgccggga tggggaggct cagtgagacg tcgctctccg gggaaggact
     3601 ccagcggatc gcggaggccc tgtctagacg atcagcaacc gagggtcgca gccacgttgc
     3661 ctggtccgat cggcaccctg tcagctcagg cgccaaggcg tggaggtcgt cgcacgtgcc
     3721 gacgagcgcg aacaggcaat tctctgatgt gttcgtgcag ccagacatca acgacgagaa
     3781 cgcgacggtg cggcgcacac ccgcgacggc ttcggctcgg gcctgcgttg atgcgcgatc
     3841 tgccggagtg gtcaggactg cgacttgctg ctgcacggtc agtgcccgct tggcaccgac
     3901 gtactgcggc agggcctcga agttgccgac cacccatcgc gcgccggtga cgaggaacag
     3961 cccgatcatc agcccggcca ccacgcgcgt caccgatgcg ggttgtgcct gcagccgtcg
     4021 cgcggcgatc gtggtggccg ggccgccgcc gactcgcagc agcaggtcgg cgaccaccct
     4081 catgaagatg gggaccacca gaatggtccc gaggccgagc agtcctacgc ccgcgaacag
     4141 gctgaggatg aggtcgtcgc ccggatcgcc ctgcccgctt cgggcgatga caacagcaca
     4201 cagggcgagt cccgcgacca acggcggcag ccgccagagc gagggccgct tcgcgtcggc
     4261 cttgcgggcc gcacgcatcg cgctgctcgt gtcgaggcga tgggggagtg cggccagggc
     4321 gacgacgacc accggcagaa cgatgatgac cgcgaactcg tcgaggacgt gcgggtgcaa
     4381 tgtctctgcg gcccagtgcc gtctagcgac gtcgatccgg gcgagcaccg gtcgagccac
     4441 ggtgaaggcc accagcccga ccgccgtacc ggcgactgcc gccgctccgg cctccacgac
     4501 cgcgaccgcg cgcgtgcggg aagggctcag gccgaggagc cggaggttgg ccagccgtcg
     4561 gtcccgcagc gcggccgaca accgtccggc ggtcgcagcc agcaccagca cgggcagtgc
     4621 gacggcgagc acgacggcca gcagcagccg ctgcatctcg acgctcgagt agaggttggg
     4681 gtgctgcgcc tgctccgccc gcgcgatcgc ggcgacgacg aggaggagca gggtgccgat
     4741 ggcggcagac agtgccacca ttgcggaccg cagccggtcg gctcgtgtcg tgccgagcgc
     4801 caggcgcagg ccgagtgcga cggctggctt catcggcccg cgcccaccgt gtcggagatg
     4861 gcaccgtcac gcatgacgag gtgccggtca agatgtgcgg ccacgaggtt gtcatgggtg
     4921 acgacgagca gggccgcccc gatggcggtc gtgagcgaga ccagcgcgtc catgacggtt
     4981 tcggcgctca ggctgtcgag agcgccggta ggctcgtccg ccagcacgat cgcgggctgg
     5041 tgcaccaggg cgcgagccac cgcggcgcgc tgtgcctgtc cgccggagac ggtgccggcc
     5101 cgtttgttgg cgaccgagtc cacgtcgagc gcggcaagca ggtccatcgc ggcggagcga
     5161 gactgcgcgc gtctgcggcc gagcagctga agcggcagca tgacgttctc ggccagggtg
     5221 agctcgggca ccaggtcgcc gaactggaag accacgccga tccgttgcag gcgcaatgcg
     5281 ctgcgcgccg attccgtcat cgtgtcgagc cgggtgccct cgacggcgac ctcgccacct
     5341 gtgggtcgga tgattccggc caggcagtgc agcagcgacg acttgcccga gcccgacggg
     5401 cccatcaggg cgacccgctc gccggtggca agcgacaggt tgaccccccg cagggcgacc
     5461 gtgtctccat aggacagccc gacctcacgt gcctcgagca cgctcgagcc gctcatcctc
     5521 ggcctgccgc ggcttcgcgg gcctggtcga ggcgttggcc ggactcctcg atccagcgca
     5581 agtcggcgtc gaggtgtgcc agctcgtagc tgatggccag cacctcggcc gggtcggcat
     5641 tcttgcgttg cctcgtgagc tcgcgcattc gtcccagatg ggtggcgcgt tgggtggcca
     5701 ggacggtttc cgcggagcgg cctgaaatca gggcgaccga tacccgagcc agtaggttgc
     5761 tggtcgcgaa ggcaccgggt ggttgtggct cgaacaccca ggcatcgacg acgtccgtcc
     5821 cggccggggt gatcgcgtat cgcttgcggt cgggcccgtc gcccgcttcg acgtcgtgaa
     5881 ggcgcgccca tccctggcgt tcgaacctgg ccagcgaggc gtagacctgg ccgaacgcga
     5941 gcgggcggat ccgcgcgaag cgcgcgtcgt actgctgctt gagggtgtag ccgtgcgccg
     6001 gttcgcgctc gagcagtgca agcaatgcgt gcgccgtggt catgatcagc aatgtacaca
     6061 cgctgtatac gctgtgtgaa tagttcgcga agattgctct ggacctgacg tcgagtggca
     6121 ccgttcgaca ctctgatctg catgacccga gcacaagggt ggtactacct gctgggcggg
     6181 atctggccgc tggtgcactt ccgctcgttc cagctggtgg cgggccccaa gcccgaccgg
     6241 ttccagaccg aggtgacctc cgggctgttc gtcgccatcg gtgcggcgct gctctcgtca
     6301 ccgagggacc cgccaccccc gcggaccgca ttggtcctgt ccgcggcgag cgggcttgcg
     6361 acggcgtacg tcgacctgcg gcatcgccga gacatccggg gcctcttcga cgtggaggcc
     6421 gcggtggaag ccgggtttgc gctggtggcg ctgaggtcgc tgctgcgggc tcgtcgaggc
     6481 cacggcacgc actgaagtca ggctgtcgcc tcccgcaatg cgcccgtgcc ggaatgtacg
     6541 gtgtccgcgg cgcggggagc cgggtcattg acggggaggt acgcggtgag ggggtcgacc
     6601 ggtcgcgcga ctggttggtc cgtgacccgg gtgctcgccc gccgtgcccg tgcgcactcg
     6661 ctgctgccgg tgaccgcggt gatgacggtg ctgctggccg ccacggggtt ggccacgctg
     6721 ctgacctact ccgcggtcgt gccgcagcag ggattggtcc gtctgctggg gtcggacacg
     6781 gcgcgggccg cggtcttcgt ggatgccgcg ctgggccagg acttcgcggc gcgcgacgag
     6841 atcgtccgcg aggcggtggg gcaccggctc ggcgcggtcc cgactcgggt ggacgtggcc
     6901 ggccagtccg cgccctaccg gttgccgccc gaggtgggtg cgcctggagt cctgaccagc
     6961 ttctggttcc tcgacgacct ggccggccag gccaggctcg tgtcgggtcg atggccggca
     7021 cccgagccgg ccggctctcc ggtcgaggtc gcgctgagcc agccggcggc ccaacggatg
     7081 gccctcgagc cgggacgacg cctgtcgttg accagcgcga cgttcgcccc tgccgtcgac
     7141 gtggtcgtga ccggggtcta cgagccggtc gacccggccg gcccggtcgt ccggctcgac
     7201 cccaccggcg gtgccggggt gaccgaaggc gagttcacca gcctcggccc gctcgcggtt
     7261 gcccccgacg tgttcatggc gcgcttcgcc gcgagcgcgc gggtgtcctg gctggcgcaa
     7321 ccggtcctca ccgggctgga cccggcgggc ctggcgcccc tgcagggcga catggcagcc
     7381 ctccgcgccg atctctccgc agcgagcgtg ttcaacggcg ccgtccaggt gcggaccgga
     7441 ctggacggtc tcatgaccca ggcgctgcgc tcgctggtcg tgagccggtc aaccctcccg
     7501 gtcccgctcc tgctcctcgc cgtactcgcc ttctgtgcca tcgcgtacgt cgggtcgctg
     7561 ctgcacgagc gtcgacgcgg acaacgagac ctgctgcgcg cccggggagc ctctcggtct
     7621 cagctcgtga ccgcggccct gctcgagggg ttggccatcg ctggtccacc cgcgctcgtg
     7681 gcgccgttcc tggcgacggg gctgctgcgg tgggcctcca atccgttggg gatcggtgac
     7741 ggcgccggac tgtccgtcgc gctgtcgcgg ggcacgtggg cctggatggt cgcggtggca
     7801 ctggccgcca ccctcacgct cgccgcgcct gccctggtac cggccggcac cggcggccgc
     7861 gggcgatggc gcgggcgggt caggccgggt gtcgaacgcg ccggcgtcga catcgcgctg
     7921 ctgctcgtgg ccggtgcggg ctatctccag ctgcgcgccg cgggctcgcc gtttgccgcc
     7981 gccgacctgc gggaggtccg ggccgatccg gtcttcgtcc tcgctccggt gatcctgctc
     8041 gtggccgtcg cgctgctggg gctgcgcctg ctacccgtcc tggctcggct ggccgggcac
     8101 ctcgccggtg ccggtcgctc ggcctctcct gcactcggcg cctggccggt ggctcggcga
     8161 ccccgccgat acgccacgtc attgttgctg ctcgtcctgg cactgtcagt cgggctgatg
     8221 tccgccgtcg cgaccgcgtc gggacaacgt tcgcgggccg accaggccaa ccaccaggtc
     8281 ggcagcgacg tgcgggtgga gttggagacg acccgggccg cagaccagga cggcgttgcc
     8341 cggctcgcca ccctgccggg tgtcgcggcg acgctgcctg ctgcgcgcga gcaggtcgac
     8401 accgggccgg gtcccgacac caccctgctg gcactggacg ctgggcaggc cagtgacgtc
     8461 atgctgctgc gggcggacct gctcgaccgg ccgaccgaag acgcctttgc cgccatgcgg
     8521 gcgccgctcg acgaggcggc cgtggcgttg ccggacgacg cgcgggcgct gcgaggccag
     8581 gtcagcctga ccgtcgtcgc aggtgcggat gagcagcccg acgctctgct cgccgggtcg
     8641 gtctcgatcg tcgtgcgcga ccgcgcggac ctcgtgcacg tggtgccctg ggccgagccg
     8701 tccaacgacg gcgccccgac ccgggtgacc gcggcgctgc cggccggcag caccgccctg
     8761 ctggggtttg tcttcggcta caccgtgccc ccgcggctca tcgcgcaggg gcccagcgac
     8821 gggcatgcca gcgtccggat cagcggcctc gagctggacg acggccgacc cgtgcggatg
     8881 ggtacgacgg agtggtccac ggccgtcgac cattcggggg cgcgcagctg ggcgccggtg
     8941 caggtgagcg ccggcgacgc gctggaggtc gcgttcggca tcggccagcc gtcggcccag
     9001 gtgccggtgc tcgcaggggc acccgagcag ctggaccggc cgatcacgtt gaccgccatc
     9061 ggttcgcaac agctgctgcg gagccacgac gtggccgtcg gagatctcgt aggtgctcgc
     9121 atccgcgggg tcaccaccca ggtccggctg gccgcagccg ccgtcgacgt accgaccgtg
     9181 tcgagcagtg aggccctggt ggtcgacctg cccacggtgc agcggttgct cctgcgtgag
     9241 tccgggcgca ccatcgcgcc gtcggaggcc tggctgcgcg ccgagccggg ccagacccag
     9301 gcggtcgcgg ccgcgatccg ggaccaaccc ggggccgcgg tggccgtggt cgaccgcgag
     9361 tcggtgcgcg cggcgctcga cgaggacccg ctgagcgtgg cgttccaggg cgcgctgcgc
     9421 atcgccctcg tcggaggggt gctgttcgcc gtcgtcgggt tcgtcgtcag tgcgctgctc
     9481 tcggtggacg agcgccgcga cgagctggcc gtgctgcgcg cgctgggcat gcgcccgagt
     9541 gacctgtcca agaccatgct ggtcgagcag gcgctggtcc tggggctggg caccgtgctc
     9601 ggtctggtcg tcggtctggt cgtgagcctg ctcgtggttc cgttgaccac ggtgagctcg
     9661 cggaccggcg ccgtcatgcc gccgatcagt ctccaggtgc cctgggtcca ggcggcgacc
     9721 cttgccatgg ccctggccgc ggtgatgcta gtggtggtcg tcgggggggt cacgacgctg
     9781 gttcggggcc ggctcggccc cacgttgcgc actcggcagg cgccatgacg tcgcggatcg
     9841 tgatcaggcg actgcgtcga gacggtagag ggctgcctct cttctacgcg gtgctggtcg
     9901 tgctggtggc cgcgctgtcc gtggcggcac ccctcgtgct gaggtccgcg gccggtcagg
     9961 cgctgcgcga cgaggtgacc cgagccgacg accaggtgcg cacgatcacg gtgcgacaga
    10021 gcgtgtcctt cagaggcgct cgcctcgagg ggcatgcaag acccgccgtg gcggcgacgc
    10081 gggtcaagct gcgccagggc atggcgccat cgctgcgtgg cgttgtgggc accggcacct
    10141 acgccgccca gtccacgcct tatgacgtgc tcgatgcccg caccggagcg cggcccgacc
    10201 ccacgagcca ccagaagctg ctgctgcgag cccagagcga ggcgctcgag cgggttcagt
    10261 gggtccgcgg tgcggcaccg gtcggctatg acctcaccga ggtggagggc cgctcgacgc
    10321 cgcgtgtcca ggtcgccctt gagaccacgg tcgcggccgc cctgcacgtc gacgtcgatg
    10381 acgtgctgtc cctgacccct gagcggttct cccagggtat gaccgtgcgg gtcactgggg
    10441 tctttgccgt cgagcgcccc gaccggtggt tctgggacgg ggagccccga gtgctcgtcc
    10501 cggctgtcaa agacgacggc aatacgcggg tcatcaccgg tgctgcgctg ttcgacccgg
    10561 gggcgatcga ggtcgtcgac gccctagcct tccagtccct cgacgtggcc taccggttcc
    10621 cggtcaccat cgctgccctt gacaccgtct cggctcccca ggcggtcacc gcactcgggg
    10681 cctaccagtc ctgggtgcgc cagaacgccg gcccaggctt cgacgcgagc agccggctgc
    10741 ccacggcggt cgagcagttc acctcggaga tgtccaccac caacgcgctc ctgtctgtcg
    10801 ccctggtcgg gttgggtgcg ggagtggcga cggcgaccgc tctcgtggcc tggctgctcg
    10861 tccggcgccg gtcgaggacg ctcgagctgc tccgggcccg gggcgcctcg ctgcgccagg
    10921 tggcgctcgt cgtcccgaga gagtgcgcgc cggtggccct cgcgctcgcg ctgagcgggt
    10981 cggtgctcgc ggtcgcggcg ctgggccggg aagccgcccg gccgatcgcg accggctggg
    11041 cgctgacgct gatcgggctg gcgttgccag ccgggatcgc cgcgtcgcgt catcgccgtg
    11101 tcgtcggtca cgacgccgcc tcggccggtg ccggccggcg agtgaccctc gagctcaccg
    11161 ttgcggcgct gaccctcggt gggctggcgc ttctgctgcg ggccggtctg cgggccagcg
    11221 ggatgaccgc cgtgcgggcc gtggttccca cgctgctcgc cgtgctgatc ggcctcctgg
    11281 tgctgcgtgg cgccagcagc gtgctcactc cgctgcgccg gcgggcatct gctgggcgtt
    11341 ctgtcgtcgg tctgctcgtc ctgaccggca tgggccgtca ccggggcgca tcggtggtgc
    11401 ctgtcctcgt gctcgtggtg acgctggcga tggccacgtt cgggctggtc accacgtcga
    11461 cactgttggc ccaccgaccc gccgatgcgc ccgcctccac ggccgcggcg ctgggcgccg
    11521 ggccgacggg ggagtcgccg atggtggcga tcctgctcac cgggctcggc ctcgccggtc
    11581 tcggatcggc ggtctgcgct ctgctggtac tggtcgtggc ccagctcggc agccggcgca
    11641 gccgcgccgg cgaccagagc ctggtcagga cgctcggctt ctcccagggc gacagccgtc
    11701 gactggtgac gctgtcactg gtgccgcagg ccgtcgtcgc gctggtggcc ggagtcctgg
    11761 ccggtctggc ggtctcggct ctcgtggtgg ccaccgtcga cctggcaccg ttcacgggtg
    11821 ccgagggcac ggcgctcgcc atcgaggccg gctggctcgc cgcgctcgtc ggcgccttcc
    11881 tccttctggc agccgtcacg gtcctcgccg aggcagccgc cgaccgccat cgtccgatcg
    11941 cctcagacct tcgagcaggg gtgccgtcat gagccgttcc acgccgtcgc ggtcggagct
    12001 gatcgacctg ccgctgccca ccaccggccg gagcacctat ggcgccgacg cccaggtggt
    12061 gtgcgacaac gtcgtacgga tctatcgctc cgccggtgtc gaggtggtgg cgctgcaggg
    12121 tctggacctg ctggtcgacc gcggtgagct cgttgccatc atcgggccct cggggagcgg
    12181 caagtcgacc ctgctgagga tcctctccgg gctcgacgtg cccaccgccg gtgcggtccg
    12241 ggtggccggc cacgacctgg gcgccatggg ccggcgagag ctgctggcct accggcgccg
    12301 tgtcgtgggg ttcgtgtggc agcagacggg ccgaaacctg ttgccctacc tgagtgcgca
    12361 cgagaacgtg gcgctgccgc tgggcatcgc tggccggcca gcccgcgagg cggccgcacg
    12421 cgccgacgag ctgctcgagc tgctcgaggt ggcccatcgt cgtgaccagc ggcccacgga
    12481 gatgtccggc ggagagcagc aacgcgcggc gatcgcggtc gcgctggcca acagcccgga
    12541 ggtcctgttc gccgacgagc cgaccggcga gctcgactcc gcgaccgcgg ccgacgtgtt
    12601 cgccgcgctg cgccacgcca acagcgagct cggcgtcacg gtcatcgtgg tcacccacga
    12661 cgcgctggtc tcctcgcagg tgcagcgcag catcagcatc cgcgacgggc ggacgagcac
    12721 ggagaccctg cgccgggtcg acgccccggt ggacgcgagc ggtcgggtcg tcgctcagga
    12781 gttcgccgtg ctggaccgtg cggggcgcct gcagctgccg gccgagttca ccgccgcgct
    12841 cgacctgcgt cggcgcgtcc gcctggagct cgagcgcgac cacatcgggg tctggcccga
    12901 tgccccggcc gccgagaccg acggcaccca cgacgatcgg ggggtgggca accatgcctg
    12961 agccgatgct gctggccgag ggactgacgc gtacctacgg cgaaggttcc cgcgccgtgc
    13021 acgcgctgcg cggtgtggat ctgcgggtgg agaccggtga gctggtcgcc atccggggtc
    13081 ggtccggctc cggcaagacc acgctgctca acctcatcgg gggactggat cgccccgacg
    13141 ctggcaccat cgtcgtcgat gggcacgagg tgaccgggtt ggacgaggac gggctgcggg
    13201 cgctgcgccg cgagaccgtc ggctacgtct tccagacctt cgcgctgatc cccaccctga
    13261 ccgccgcgga gaacgtcggc atccccctgc gactgcggcg ggtggcgccc gccgcacgag
    13321 aggcgcgggt ggcgcagctg ctggacctgg tcggcttggc cgaccacggc gcgcagacac
    13381 cggagcagct ctccggcggt cagcagcaac gggtcgcgct ggctcgtgcg ctggccgggc
    13441 agccccggct gctgctcgcc gacgagccga ccggccagct cgactcgcac accggtcggg
    13501 aggtcatggc tctgctgagt gcggtggtgc acgaggccgg agccaccgcg ctggtcgcca
    13561 cccacgaccc cggggtgctc gcggtggccg accgggtcct ggagattcga gacggcacgc
    13621 tgaccccgga cctgctggcc gcgcaagccc tggcccgcga gcaggcgatg ggtggttgac
    13681 gccccccggc gtgggcgctc gtggctaggc tgaccgcgcc ggctgcaccg cgtgtcagac
    13741 gggtgcaggt ccggccgggc gagggccgga ggtgatgctc gtggtgagta cgacgcacgg
    13801 ccagggcggc ggcgggcggc cacggctggt cttcctcgag gccacctcca cctacggcca
    13861 gcccggcatg gcggatgagt acctgctgcc ggccgagcgc ccgttctccg tcggctcgag
    13921 tcaggactgc gacgtcacgc tgctcgggct ggcgcc
//
LOCUS       DATIBB010000193         3050 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_1836690, whole genome shotgun sequence.
ACCESSION   DATIBB010000193 DATIBB010000000
VERSION     DATIBB010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3050
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1836690"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(162..1943)
                     /locus_tag="VLC50_07675"
     CDS             complement(162..1943)
                     /locus_tag="VLC50_07675"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase C-terminal domain-containing
                     protein"
                     /protein_id="HSF27378.1"
                     /translation="MLNDSVDLRRRLAAVALGEVPADLVVRGGIVVDVYSGLTHRADV
                     AVSGDRIAAVGDVGYAIGDATTVIEADGQYVVPGLIDTHIHVGGSQTPPTQLAQLLIP
                     HGTAAIGTCFYEAAAIAGVGAVRTFLDEAATTPLHILLSVFHSCYAGMGPAGNPGRVS
                     AEDLLGLLDLPEAIEIREWSVFNEKAPDDVFRAYVDRARERGLIVAGHLEGQTGRPLQ
                     ASVALGAHSDHEMGTAAEAMEKLRLGVRIQARQGAAAWDLHNVLRAVTEMGADSRYFM
                     FATDEQEAHHLAEHGHIDDLVRMAIGMGIDPVDAVRMGSQNAAEYLGLTDHLGAVAPG
                     RLAHLLLVDNLREFTVQRVVADGRLAAEDGRYLAPVTPVDYPAEWRETVHVASISPAS
                     FVIDAPGDEPVDVRVIGITPGSLLTDERRITMTPYDGQLRADPSRDLAKLCVIDRHGA
                     SGRVGRAFTQGTGLSVGAVATTVNPGLQNLMVLGVDDADMDLAARRLAELGGGIVVAI
                     GGEVAAEVALPLLGIFSDGPVEDTVAAMAAVERALLDKMDASMPGLLAAVGFATLAVS
                     IPALKVCDQGLVSVRRDSSEFVPLLYG"
     gene            complement(1979..>3050)
                     /locus_tag="VLC50_07680"
     CDS             complement(1979..>3050)
                     /locus_tag="VLC50_07680"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HSF27379.1"
                     /translation="GLYGSGKVLVLDEPTAVVSEADSEHLLQRLNALREQGMAILYVT
                     HRLTEVLTIADRVTVLRDGRKVAENARAEVTREGLIRHMTKVSASSTPTTDGTAAPGG
                     DAPASGSGSGRPHALTQASGSEVRVEALHAPPSVVQASLTLRPGRVAGIVGVQGSGHG
                     DLLHALAGLRDYDGTVDVAGRRLAPGSVASARRAGVRLVPGDRRRAAIAAHLSLADNV
                     ALSSATSSRFGFRRRRTERASAAGYVATMDVRPPSTGAKAGSLSGGNQQKIAIANALE
                     SQPRVLLLEEPTQGVDVMGKVEIRKLIRALARSGRCVAIATSEFEDLPDYVDDIYVMR
                     LGRLHGPVPPEQATYERLLHAA"
BASE COUNT          440 a         1090 c         1099 g          421 t
ORIGIN      
        1 accgagccca acaatggcgc caacaccacc gctacaccct cgacaccatc agacgcagat
       61 agagggcccc gcagcgcacc gccgccacac gcgcatgatc ggtgcgaaga cgtggtgccc
      121 gccgcgatgg tttgcgcatg atcagtgcag ggtgggtggg gtcagccgta gagcagcggg
      181 acgaactcgc tgctgtcccg gcgcaccgag accaggccct ggtcgcagac cttgagcgcg
      241 gggatcgaga cggcaagcgt cgcgaagccc accgcggcca gcaggccggg catcgaggcg
      301 tccatcttgt cgaggagcgc ccgctccacg gccgccatcg ccgccacggt gtcctccact
      361 gggccgtcgg agaagatgcc cagcagcggc agcgccacct cggcggccac ctcgccgccg
      421 atggccacca cgatcccacc gccgagctcg gccagccgcc gagcggcgag gtccatgtcg
      481 gcgtcgtcga ccccaagcac catgaggttt tgcaggccgg ggttcacggt cgtggccacc
      541 gcaccgaccg ataggccggt gccctgggtg aaggcccgtc cgacccgacc cgacgccccg
      601 tgccggtcga tgacgcacag cttggccagg tcgcggctcg ggtcggcacg cagctgtccg
      661 tcgtacggcg tcatggtgat gcgtcgctcg tcggtgagca gcgaccccgg ggtgatcccg
      721 atgacccgca catcgaccgg ctcgtcgccg ggcgcgtcga tcacgaagct ggcgggggag
      781 atggacgcga cgtgcaccgt ctcgcgccac tcggccgggt agtcgaccgg cgtgaccggc
      841 gcgaggtagc gcccgtcctc ggccgccagc cgtccgtcgg ccaccacgcg ctgcacggtg
      901 aactcgcgca ggttgtcgac gagcagcagg tgtgccagcc gacccggcgc gaccgcgccg
      961 aggtggtcgg tgagaccgag atactcggcg gcgttctgcg agcccatccg caccgcgtcg
     1021 accgggtcga tgcccatgcc gatggccatc cggacgaggt cgtcgatgtg cccgtgctcg
     1081 gccaggtggt gcgcctcctg ctcgtcggtg gcgaacatga agtagcggct gtcggcgccc
     1141 atctcggtga ccgcccgcag cacgttgtgc aggtcccagg cggcggcacc ttgacgagcc
     1201 tggatgcgta cgccgagacg cagcttctcc atcgcctccg cggcggtgcc catctcgtgg
     1261 tcggagtgcg cgcccagcgc caccgaggcc tgcagcggcc gcccggtctg gccctcgagg
     1321 tgccccgcca cgatcaggcc gcgctcgcgg gcccggtcga cataggcccg gaacacgtcg
     1381 tcgggcgcct tctcgttgaa gacgctccat tcccggatct cgatggcctc gggcaggtcg
     1441 agcagcccga gcaggtcctc ggccgagacc cggccggggt tgccggccgg ccccatgccg
     1501 gcgtagcagg agtggaacac cgacagcagg atgtgcagcg gcgtggtggc ggcctcgtcg
     1561 aggaatgtcc gcacggcgcc cacgccggcg atggctgccg cctcgtagaa gcaggtgccg
     1621 atggccgccg tgccgtgcgg gatgagcagc tgggccagct gggtgggcgg ggtctgcgag
     1681 ccacccacgt ggatgtgggt gtcgatcagg ccgggtacga cgtactgccc gtcggcctcg
     1741 atcaccgtgg tggcgtcgcc gatcgcgtag ccgacgtcac ccacggccgc gatccggtcg
     1801 ccggacacgg caacgtcggc gcggtgggtc agcccggagt agacgtcgac gacgatgcct
     1861 ccgcgcacga ccaggtcggc cggcacctca ccgagggcca ccgccgcgag gcgacgacgc
     1921 aggtcgacgg aatcgttgag cacgacagat cggtcctttc agctgacggt cttcagggct
     1981 aggcggcgtg gagcagccgc tcataggtgg cctgctccgg cggcacgggt ccgtgcagtc
     2041 ggcccagccg catcacgtag atgtcgtcga cgtagtcggg caggtcttca aactccgagg
     2101 tggcgatcgc gacacagcgt ccgctgcggg ccagggcccg gatcagcttg cggatctcga
     2161 ccttgcccat gacgtcgacg ccctgggtgg gctcctccag cagcagcacc ctgggctggg
     2221 actccaatgc gttggcgatg gcaatcttct gctggttgcc gccgctgagc gagccggcct
     2281 tggcgccggt cgacggcggt ctcacgtcca tcgtcgcgac atagccggcg gcgctggccc
     2341 gctcggtccg gcgccgacga aaaccgaacc gactggaggt ggcgctggac agggccacgt
     2401 tgtcggcgag gctgaggtgc gccgcgatcg cggcgcgccg ccggtcaccg ggcacgagac
     2461 gtacgccggc ccggcgcgcc gaggcgaccg agccgggggc cagcctgcgt ccggcgacgt
     2521 cgacggtgcc gtcgtagtcg cgcagcccgg ccaacgcgtg cagcaggtcg ccgtgacccg
     2581 acccctgcac gccgacgatg ccggccaccc ggcccgggcg cagcgtcagg ctggcctgca
     2641 ccaccgacgg cggcgcatgc agcgcctcga cccgcacctc gctgccggag gcctgcgtga
     2701 gcgcgtgagg gcggcccgag cccgagccgc tggcgggcgc gtcgccgccg ggggccgccg
     2761 taccgtccgt ggtaggtgtc gagctggcgc tgaccttggt catgtgccgg atcagcccct
     2821 cgcgggtgac ctcggcgcga gcgttctcgg cgaccttgcg cccgtcgcgc agcacggtca
     2881 cccggtcggc gatggtgagc acctctgtga gccggtgggt gacgtagagg atcgccatcc
     2941 cctgctcgcg caaggcattc agccgttgca gcaggtgctc ggagtcggcc tcggagacca
     3001 ccgcggtggg ctcgtcgagt accagcacct tgcccgagcc atagagccca
//
LOCUS       DATIBB010000194         2138 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_1967944, whole genome shotgun sequence.
ACCESSION   DATIBB010000194 DATIBB010000000
VERSION     DATIBB010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2138
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1967944"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..903
                     /locus_tag="VLC50_07685"
     CDS             <1..903
                     /locus_tag="VLC50_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020672102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HSF27380.1"
                     /translation="HVAGLQVLVRSLLAGTEPVVLDLAGGFDPVAFADGSRMISAGVS
                     GGAVGAGAGRRYTALVPTQLRRLLSAGDDVVAALASYDAVLLGGAAAPPDLLAAARAV
                     GVRVVTTYGMSETCGGCVYDGVPLDGVRVRLGEDGRVELGGTMVFAGYRGRPDLTSAA
                     RRVDADGVAWHLTQDLGVLSDDGRLDVLGRVDDVIVTGGEKVAPALVERALASYAGIR
                     ELAVVGVPDPEWGQRVVAVVVPADAAAAPSLEKLRAHGAHRLPPYALPRSLVIADAIP
                     LLGSGKPDRVALRALAAPAAVQVG"
     gene            916..1851
                     /locus_tag="VLC50_07690"
     CDS             916..1851
                     /locus_tag="VLC50_07690"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010148160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HSF27381.1"
                     /translation="MSARQVSEIFDPALWREVDGFAFTDITYHRHVRDGVDQGTVRIA
                     FDRPDVRNAFRPHTVDELYRALDHARMTPTVGCVLLTGNGPSARDGGWAFCSGGDQRI
                     RGRSGYQYADGETADTVDQARVKAAGGRLHILEVQRLIRTMPKVVVAVVPGWAAGGGH
                     SLHVVCDLTIASREHARFKQTDADVGSFDAGYGSAYLARMVGQKRAREIFFLGRTYSA
                     DEMAAMGAVNLVADHAALEAVALEVAAEINGKSPTAQRMLKFAFNLVDDGLVGQQVFA
                     GEATRLAYMTDEAVEGRDAFLQKRDPDWSPFPWYF"
     gene            1909..>2138
                     /locus_tag="VLC50_07695"
     CDS             1909..>2138
                     /locus_tag="VLC50_07695"
                     /inference="COORDINATES: protein motif:HMM:NF014045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HSF27382.1"
                     /translation="MISPESHVAIYIDFDNVVISRASQVKDTGASATVAIDVLLDFAT
                     RYGRLTISRAYADWSMKKNADYKQQLVARAVEL"
BASE COUNT          276 a          719 c          812 g          331 t
ORIGIN      
        1 cacgtcgccg ggctgcaggt gctggtgcgg tcgttgctcg ccggcaccga gccggtggtg
       61 ctcgacctgg ccggcgggtt cgacccggtc gcgttcgccg acggttcgcg catgatcagt
      121 gcaggggttt cggggggtgc ggtgggtgcg ggggccgggc ggcggtacac ggcgctggtg
      181 ccgacccagc tgcggcggct gctgtccgcc ggcgacgacg tggtggccgc gctggcgtcg
      241 tacgacgcgg tgctgctcgg cggcgcggcc gctcccccgg atctgctggc cgcggcacgg
      301 gcggtcgggg tgcgggtggt cacgacctac gggatgagcg agacgtgcgg cggctgcgtc
      361 tacgacgggg tgccgctcga cggggtgcgg gtgcggctcg gcgaggacgg gcgggtcgag
      421 ctgggcggga cgatggtctt cgccggctac cgcggccggc ccgacctgac ctcggccgcc
      481 cgacgggtcg acgccgacgg cgtggcctgg cacctcaccc aggatctcgg cgtgctttcc
      541 gatgacggcc gactcgacgt gctgggccgc gtcgacgacg tgatcgtcac cggcggcgag
      601 aaggtggccc cggccctggt ggagcgggcg ctggcgtcgt acgccgggat ccgcgagctc
      661 gccgtcgtcg gggtgcccga cccagagtgg ggacagcgag tggtcgcggt tgtcgtgcct
      721 gccgacgctg cggcggcgcc cagcctcgag aagctgcgag cgcatggcgc gcaccggctg
      781 ccgccgtacg cattgcctcg atctttggtg atcgccgacg cgatcccgtt gctgggctcg
      841 ggcaagcccg accgggtcgc gctgcgggcg ctggccgccc ccgccgcggt gcaggtcggc
      901 tgacaggatg ccggcatgag cgcacggcag gtgtccgaga tcttcgaccc ggcgttgtgg
      961 cgggaggtcg acgggttcgc gttcaccgac atcacctatc accggcacgt ccgcgacgga
     1021 gtggaccagg gcacggtgcg gatcgcgttc gaccgacccg atgtgcgcaa cgcgtttcgc
     1081 ccgcacaccg tggacgagct ctaccgcgcg ctcgaccacg cccggatgac tcccaccgtc
     1141 gggtgcgtgc tgctgaccgg caacgggccc tccgcgcgtg acggcgggtg ggccttttgc
     1201 tccggcggcg accagcggat ccggggacgg tcgggctatc agtacgccga tggcgagacc
     1261 gccgacaccg tcgaccaggc ccgggtcaag gccgcgggcg ggcggctgca catcctcgag
     1321 gtgcagcggc tgatccgcac catgcccaag gtggtggttg cggtggtgcc gggctgggcg
     1381 gccggcggcg ggcactcgct gcacgtcgtc tgtgacctga ctatcgcctc gcgcgagcac
     1441 gcccggttca agcagaccga cgccgacgtg ggctcgttcg acgccggcta cggctcggcc
     1501 tatctcgcgc ggatggtcgg gcagaagcga gcgcgggaga tcttcttcct cgggcgcacc
     1561 tattccgccg acgagatggc cgcgatgggc gcggtcaacc tggtcgccga ccacgccgct
     1621 ctcgaggcgg tcgccctcga ggtggccgcc gagatcaacg gcaagtcgcc gaccgcccag
     1681 cggatgctga agttcgcgtt caacctcgtc gacgacgggc tggtcggcca gcaggtgttc
     1741 gccggcgagg cgacccggct ggcctacatg accgacgagg cggtcgaggg tcgcgacgcg
     1801 ttcctgcaga agcgcgaccc cgactggtcg ccgttcccct ggtacttctg accccgccgc
     1861 ctgcacaggc gagcggcctc gtaggcattg agggctaggg tcctgagcat gatcagtccc
     1921 gagtcgcacg tggcgatcta catcgacttc gacaatgtgg tcatctcgcg cgcgagtcag
     1981 gtcaaggaca ccggggcatc agcgacggtc gcgatcgatg tgctgctcga ctttgccact
     2041 cgctacggac gcctcacgat ctcccgggcg tacgccgact ggtcgatgaa gaagaatgct
     2101 gactacaagc agcagctcgt cgcccgtgcg gtcgagct
//
LOCUS       DATIBB010000195        13123 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_1179597, whole genome shotgun sequence.
ACCESSION   DATIBB010000195 DATIBB010000000
VERSION     DATIBB010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 13123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13123
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1179597"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..972)
                     /locus_tag="VLC50_07700"
     CDS             complement(<1..972)
                     /locus_tag="VLC50_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020389406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HSF27383.1"
                     /translation="MTPAAIDSGNTAWLLVSAALVLLMTAGLGFFYGGLVRAKSALNM
                     IMMSFLAIGVVTVVWVLGGYSLAFAGGRNPLVGDLSEVGLGGLLQTHHGTVPELAWAA
                     FQLMFAIIAVALVSGAVAERVKPGAWVGFVAAWTTLCYVPVAHWVFDADGWIVDRLGA
                     LDFAGGTAVHINAGAAALALALVVGRRVGWGDEPMRPHNIPLVLLGAGLLWFGWFGFN
                     AGSALAADGLAALALVNTQVAAAAAVLGWSVTERVRDGHVTTVGAASGAVAGLVAITP
                     AAGSITPAAAVGLGATAGVVCALAVGLTYRWGFDDSLDVVGVHLVGGM"
     gene            complement(1158..2342)
                     /gene="ftsY"
                     /locus_tag="VLC50_07705"
     CDS             complement(1158..2342)
                     /gene="ftsY"
                     /locus_tag="VLC50_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019523258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HSF27384.1"
                     /translation="MELFVIAALVLAVVLVLTLGFVLLRPSGRGRQAPSAPRPGIDYA
                     PGVGDDDQPPRETPRRTVEDVPLPGAGTTTLDEPAPESVVEAPAPVAGRMVRLRARLA
                     RSQTTLGRGLLALVSRAHLDDATWDEIEDTLLAADVGVGPATELVDRLRTRVRVESVD
                     TPERARALLREELLALVDPSMDRELRTTRVEGRPAVVLVVGVNGTGKTTTVGKLARVL
                     VAEDKEVVLGAADTFRAAAAEQLQTWGERVGVVTVRGPEGADPASVAFDAVKVSTEAE
                     ADVVLVDTAGRLQNKAGLMDELGKVKRVLERRGPVDEVLLVLDATTGQNGLVQARVFR
                     EAVDVTGIVLTKLDGTAKGGIVVAVQRELGVPVKLVGLGEGPDDLAPFDPATFVDALL
                     GD"
     gene            2445..3743
                     /locus_tag="VLC50_07710"
     CDS             2445..3743
                     /locus_tag="VLC50_07710"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HSF27385.1"
                     /translation="MPVTARHARPRAPRRRRRVIVALLSVAVLVAGTVGGALLWANWQ
                     LSQVPTFSARPPVVHDGAAPPTEAVEEPLRLPAAMDGVSTFLVFSTGSRDMSAEDARR
                     YGVPDLAYRGDDSLTDSIILAVLDAPARQLSMLSIPRDTWIERRGTRINEVFLNHGAA
                     ALAQDVTRLTGLPVNHMVAVSFTAAARLTDVVGGIDLQIPTPMRDRKSHLLLPDSGCI
                     HMDGRTALAFARSRHTQTMDVDGSWRGDPSASDFGRSTRQQAVITAALGKLLSPRLPL
                     YLPDLAATARATLTIDAGLDMGALFDTGRVLATGDALAVHHFGLPSRVGKVGEASVVF
                     TDAVAARRVLAPLIAAVPTAEWPRWLGPEPRSSSPTPTPTDTAVEDRSSTTVVGSDTQ
                     LAVEGFDTGPNTGYRTCSDGLTPPAQPTSQNDPRFFRSSR"
     gene            complement(3750..7328)
                     /gene="smc"
                     /locus_tag="VLC50_07715"
     CDS             complement(3750..7328)
                     /gene="smc"
                     /locus_tag="VLC50_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015883036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HSF27386.1"
                     /translation="MHLKSLTLKGFKSFASATTLRFEPGITAVVGPNGSGKSNVVDAL
                     AWVMGEQGAKSLRGGTMEDVIFAGTPARAPLGRAEVSLTIDNTDGALPIDYAEVTLTR
                     LMFRNGGSDYAINGTACRLLDIQELLSDSGLGREMHVIVGQGRLDAVLSATPEVRRGF
                     IEEAAGVLKHRKRKDKALRKLDAMAANLTRLTDLTAELRRQLKPLGRQAEVARRAAVI
                     QADARDARLRLLADELAALSATLEAEVADETALKARRAELETALAAGQAREQALETAA
                     REAAPVLAVAQETWYRLSGLRERLQGTVTLAAERVRHGTEAPAEAPAGRDPDELDAAA
                     AAARAEQARLAAQVAADGEALAAAVAIRKQAEAAHAEEERRMAAAARAAADRREGLAR
                     LAGKVAAAHSRVEAGEAELGRLRAAAAEAEERATRARAEFAALESQVAGLDQGEEGLD
                     AEHESASAALDEASARADELRAAEREAERDRTAWTARREALELGLARRDGAGVLLAAG
                     DRLSGVLGSVAALLRVQAGYSSAVAAALGAAADAVAVDSVDAAVDSLRLLKDDDAGQV
                     RLLVGAAGNDLPMVDRPTVPAGAHAVLDLLEAPEPLRPALHHLLVDVVVVDGGLAEAR
                     ALLARRPDLTVVTTDGDVLGRLRARGGSASAPSLLEVQAAVDEATTRLDEALARGQRL
                     RFELQAASEAVQQAQRRVDAALSRLHESDARLAAVAEQLGQLGSLARAAAGEADRLTR
                     AAAAAEAALATDRAASAELDERLAGAEAAPAPQEEPDGAERDRLAQAATEARARELEA
                     RLAVRTGEERLRATAGRAEALERSARSEREARAREAARRERMARSAATAEAVRRGALL
                     VLDRLERSLAAAATERTDLEAARVEREATAAQVRTQVRELAEALSALVDTAHRDEVAR
                     AQQRLRIETLQARSVEEYGVDPAVLLDEYGPHQPVPPSPPAPGDPEPEVAPAPVPFVR
                     AEQEKRLRAAERALAQLGKVNPLALEEYAALEERHAFLGEQLEDLRSTRRDLLEIVRE
                     VDERIEAAFVEAYRDTEREFRDVFARLFPGGEGRLVLTDPDDLLATGVEVEARPPGKK
                     VKRLSLLSGGERSLTAVALLVAIFKARPSPFYVMDEVEAALDDTNLGRLLVLFEELRE
                     SSQLIVITHQKRTMEIADSLYGVSMRGDGVTTVISQRLRETEPASA"
     gene            complement(7439..7642)
                     /locus_tag="VLC50_07720"
     CDS             complement(7439..7642)
                     /locus_tag="VLC50_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013133009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF27387.1"
                     /translation="MAKALLGHVGGTDLRLVDEMRRLRQRVRDLEDELGRLQRENDAL
                     AAAVQDAHADELLTLPSQAPAYS"
     gene            complement(7826..8704)
                     /gene="mutM"
                     /locus_tag="VLC50_07725"
     CDS             complement(7826..8704)
                     /gene="mutM"
                     /locus_tag="VLC50_07725"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018220742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HSF27388.1"
                     /translation="MPELPEVEVVRRGLARWVAGRVVADVEVRHPRAVRRHVPGATDF
                     AARLSGRRLGQAHRRGKYLWLTLDDDPETTAGRGEALVAHLGMSGQLLVEADDAPDET
                     HLRVRLRFADRGPQLRFVDQRTFGGLALDATVDGVPASVAHIARDPLDPDFDDAGFVA
                     ALRSRRTGLKRALLDQTLVSGVGNIYADESLWRARLHGARATATLRPVDARRVLAAVR
                     AVLTEALAAGGTSFDALYVDVNGTSGWFERSLAVYGRAGQPCPRCATPVRREPFMNRS
                     SYSCPRCQPRPRHPVA"
     gene            complement(8704..9447)
                     /gene="rnc"
                     /locus_tag="VLC50_07730"
     CDS             complement(8704..9447)
                     /gene="rnc"
                     /locus_tag="VLC50_07730"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009068882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HSF27389.1"
                     /translation="MTAVSACPELEERLGVALPEALLGRALTHRSYAYENGGLPTNER
                     LEFLGDAVLGLVVTDTLFRANPETAEGQLAKLRAAVVNMRALADVGRSLELGRFIKLG
                     RGEQATGGRDKSSILADTLEALFGAVYIDQGLQTASELIHRLLDPLIAESATLGAGLD
                     WKTSLQELTAIVGLGVPDYVVVDSGPDHDKTFQATVRAGERDFGVGTGRSKKEAEQHA
                     AAAAWAAIRAEFDVVGAPAQGRSPSYDGG"
     gene            complement(9444..9617)
                     /gene="rpmF"
                     /locus_tag="VLC50_07735"
     CDS             complement(9444..9617)
                     /gene="rpmF"
                     /locus_tag="VLC50_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006139588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HSF27390.1"
                     /translation="MAVPKRKMSRANTRARRSQWKATAPTLVTCEQCRAPKLPHTACP
                     TCGTYKRRPVVGA"
     gene            complement(9620..10210)
                     /locus_tag="VLC50_07740"
     CDS             complement(9620..10210)
                     /locus_tag="VLC50_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012853757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceD family protein"
                     /protein_id="HSF27391.1"
                     /translation="MTQQSRRNVHRLDPASPLVADTRELGRRPGSMRRLLRTVAAPAG
                     LGNDVIGVPAGIDLELALRLEAVQEGVLVSGTAQAVATGECVRCLGPLQVDLEVELQE
                     LFAYEEARHHHEEVAGESDDAELAVLEGDLVDLEPLLRDAVVLALPLQPVCRDDCSGL
                     CPRCGARLDDDPGHHHDDVDPRWAALQSVFDDERES"
     gene            complement(10277..10822)
                     /locus_tag="VLC50_07745"
     CDS             complement(10277..10822)
                     /locus_tag="VLC50_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF27392.1"
                     /translation="MDVDDKLDELVALVENARSMPLSASCIVNRGDLLALLDEMRQLL
                     PEEFRHAQLLLADRDAVVEEGRREVARLLEQARAEQARLVSESVVWAEARQQAQEIVD
                     SAREETDGMRHEVDDYVDTKLANFEVVLTKTLAAVRRGREKLHGRHELDELGALAGEP
                     AQLGVLAEPLARHRSGEVDEP"
     gene            complement(10889..11371)
                     /gene="coaD"
                     /locus_tag="VLC50_07750"
     CDS             complement(10889..11371)
                     /gene="coaD"
                     /locus_tag="VLC50_07750"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HSF27393.1"
                     /translation="MRRCVCPGSFDPVTNGHLDVITRAAELYDEVVVAVLVNIAKRGL
                     FDVDERLELLGVVTKDLPAVRIETFDGLLVDFCREREIPVILKGLRAVSDFDYELTMA
                     QMNHRLAGVETLFIPTNPEYSYLSSSLVKEVAGLGGDVAGLVPDVVNARLRERLGRPA
                     "
     gene            complement(11429..11980)
                     /gene="rsmD"
                     /locus_tag="VLC50_07755"
                     /pseudo
     CDS             complement(11429..11980)
                     /gene="rsmD"
                     /locus_tag="VLC50_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010061993.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
     gene            complement(11991..>13123)
                     /locus_tag="VLC50_07760"
     CDS             complement(11991..>13123)
                     /locus_tag="VLC50_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018385049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HSF27394.1"
                     /translation="ALLTGSATTAHRRAALLDTVSGQAGIVIGTHALLQERVDFLDLG
                     LVVVDEQHRFGVEQRDALRAKAATPPHVLVMTATPIPRTVAMTVFGDLEVATLTELPL
                     GRAPISTHVVPAAEKPHYLDRAWVRVREEVQGGHQAYVVCPRIGDDGEAGPEGDQDQP
                     DSPADPAEVTARRPPKAVVDVAAELERGPLAGLRLAVLHGRLPADDKDAIMRGFAAGA
                     IDVLIATTVIEVGVDVPNATAMVVLDADRFGVSQLHQLRGRVGRGTAPAVALLVTEAE
                     PGSPARERLDAVAATLDGFELSMVDLRSRREGDVLGAEQSGTHSSLRLLRVTRDEAVI
                     RAAREEAGALIDADPALEAHPVLRAVIEQTLDPYRVDYLEKG"
BASE COUNT         1728 a         4935 c         4776 g         1684 t
ORIGIN      
        1 catgcccccc accaggtgca cgccgacgac gtcgagggag tcgtcgaagc cccaccggta
       61 ggtgagtccg acggccagcg cgcacaccac accggccgtc gccccgagcc cgaccgcggc
      121 cgccggggtg atcgagccgg ccgccggggt gatcgcgacc aggccggcca ccgcgccgga
      181 ggcggcgccc acggtggtga catggccgtc gcggacccgc tcggtcaccg accagccgag
      241 caccgctgcc gccgcagcga cctgggtgtt gacgagcgcg agcgccgcca ggccgtccgc
      301 cgcgagggcc gacccggcgt tgaagccgaa ccagccgaac cacagcaggc ccgcgcccag
      361 cagcaccagc gggatgttgt gcgggcgcat cggctcgtcg ccccacccga cccgccgccc
      421 gacgaccagg gccagggcga gggccgccgc gcccgcattg atgtgcaccg cggtgccgcc
      481 ggcgaagtcc agggccccga ggcgatcgac gatccagccg tcggcgtcga agacccagtg
      541 ggcgaccggg acgtagcaca gcgtcgtcca cgccgcgacg aacccgaccc acgcgccggg
      601 cttgacccgc tcagcgacgg cgccgctgac cagcgccacg gcgatgatgg cgaacatcag
      661 ctggaaggcc gcccacgcca gctccggcac cgtgccgtgg tgcgtctgca gcagcccacc
      721 caggcccacc tccgacaggt caccgacaag cggattgcgc ccgccggcga acgccagcga
      781 gtagccgccg agcacccaca ccaccgtgac gacaccgatc gccaggaagc tcatcatgat
      841 catgttgagg gcgctcttgg ccctgaccag gccgccgtag aagaagccca ggcccgccgt
      901 catgagcagg accagggcgg cgctgaccag cagccaggcg gtgttaccgg agtcgatcgc
      961 agctggggtc accgcacacg cctctctgtc gcagcgcggt gggtccgcgg ccgggcgagc
     1021 ggagcctgct gcccgcaggt gacggacggt gacgtgtcgt gtttcgagcg tgtgacgact
     1081 agagccggcg tgtgacgttc atgtgacgga cgtccagatc gcgagacgcc gccgctgccg
     1141 accccgcggg cggcgagtca gtcgccgagc agggcgtcga cgaaggtggc ggggtcgaac
     1201 ggtgcgaggt cgtcggggcc ctcgcccagc cccaccagct tcaccggcac cccgagctcg
     1261 cgttgcaccg cgacgacgat gccgcccttg gcggtgccgt cgagcttggt gagcacgatg
     1321 ccggtgacgt cgaccgcctc gcggaacacc cgcgcctgca ccagaccgtt ctgtcccgtg
     1381 gtcgcgtcga gcacgagcag cacctcgtcg accgggccgc ggcgctcgag cacccgcttg
     1441 accttgccga gctcgtccat gaggccggcc ttgttctgca gccggcccgc cgtgtcgacg
     1501 agcacgacgt cggcctcggc ctcggtgctg accttgaccg cgtcgaaggc caccgaggcc
     1561 gggtcggcac cctcggggcc ccgcaccgtc accacgccca cccgctcccc ccaggtctgc
     1621 agctgctcgg cggccgcggc gcggaacgtg tcggccgcgc cgagcacgac ctccttgtcc
     1681 tcggcgacga gcacgcgggc gagcttgccc accgtggtgg tcttgccggt gccgttgacc
     1741 cccacgacga gcaccaccgc ggggcggccc tcgacgcggg ttgtgcgcag ctcgcggtcc
     1801 atggacgggt cgaccagggc gagcagctcc tcgcgcagca gcgcgcgggc ccgctccggg
     1861 gtgtcgacgc tctcgacccg cacccgggtc cgcagccggt cgacaagctc ggtggccggc
     1921 ccgaccccca cgtcggccgc gagcagggtg tcctcgatct cgtcccaggt ggcgtcgtcg
     1981 agatgggcgc gggagaccag cgcgagcagg ccccggccca acgtggtctg cgaccgagcc
     2041 agacgtgccc gcagccgcac catgcgcccg gcgaccggag ccggcgcctc gaccacggac
     2101 tccggtgccg gctcgtcgag ggtggtcgtg ccggcgccgg gcaacgggac gtcctcgacg
     2161 gtgcgccgcg gggtctcacg cggcggctgg tcgtcatcac ccactcccgg ggcgtagtcg
     2221 atcccaggcc gcggcgcgct cggggcctgg cgcccccgcc cagacggtcg gagcaggacg
     2281 aacccgagcg tgagcacgag tacgacggcg agcacgaggg cagcgatgac gaaaagctcc
     2341 atggccgcgc cagtctcgca gacggcgctc cgcctcgcgc ccggcctgcc gtgacagaat
     2401 tgccgcttcc ggccggaacc gtccggcttg accaaagtga gcccatgccc gtcaccgccc
     2461 gtcacgcccg gccgcgcgcc ccccggcgcc gacgacgcgt gatcgtggcg ctgctctcag
     2521 tggcggtgct cgttgcgggc accgtcggag gcgcgctgct gtgggccaac tggcagctct
     2581 cgcaggtgcc gacgttctcg gctcgcccac ccgtggtgca cgacggcgcg gcgccaccca
     2641 ccgaggcggt cgaagagccg ctgaggttgc ccgcggcgat ggacggggtc agcacgttcc
     2701 tcgtcttcag caccggcagc cgggacatgt ccgccgagga cgcccgccgt tacggcgtac
     2761 ccgacctggc ctacagaggc gacgactcgc tgaccgacag catcatcctg gccgtcctcg
     2821 acgcacctgc ccgccagctc agcatgctct cgatcccgcg ggacacctgg atcgagcggc
     2881 gtggcacgcg catcaacgag gtgttcctca accacggtgc cgccgcgctc gcccaggacg
     2941 tcacccggtt gaccgggctg ccggtcaacc acatggtggc ggtcagcttc accgccgcgg
     3001 ctcggctcac cgacgtcgtc ggtgggatcg acctgcagat cccgaccccg atgcgtgacc
     3061 gcaagagcca cctcctcctg cccgactccg ggtgcatcca catggacggg cgcaccgcgc
     3121 tggccttcgc ccggtcgcgc cacacgcaga ccatggacgt ggacggctcc tggcgcggcg
     3181 acccgtcggc cagcgacttc ggccgctcga cccgccagca ggccgtcatc accgccgcgc
     3241 tcggcaagct gctctcgccg cggctaccgc tctacctgcc cgacctggcc gccaccgctc
     3301 gggcgacgct caccatcgac gcggggctgg acatgggcgc cctcttcgac accggccggg
     3361 tgctggccac cggcgacgcc ctggccgtgc accatttcgg cctgccctcg cgggtgggga
     3421 aggtcggtga ggccagcgtg gtgttcaccg acgcggtcgc cgcccggcgg gtccttgcgc
     3481 cgctgatcgc cgcggtgccg accgccgagt ggccgcgctg gctcggaccc gagcctcggt
     3541 cgagctcgcc gacccccacg cccaccgaca cggcggtcga ggaccggtcc tccacgaccg
     3601 tcgtgggctc ggacacccag ctggcggtcg agggcttcga caccgggccc aacaccggct
     3661 accgcacctg cagcgacggg ctcaccccgc ccgcgcagcc cacctcgcag aacgacccgc
     3721 ggttcttccg ctcgtcgcgc tgacccggct caggccgagg ccggctcggt ctcgcgcagc
     3781 cgctggctga tcaccgtggt gaccccgtcg ccgcgcatcg acacgccgta gagcgagtcc
     3841 gcgatctcca tcgtccgctt ctggtgggtg atcacgatga gctgcgacga ctcgcgcagc
     3901 tcctcgaaga gcacgagcag tcggccgagg ttggtgtcgt cgagcgcggc ctcgacctcg
     3961 tccatcacgt agaacgggct gggtcgggcc ttgaagatcg cgacgagcag ggcgaccgcg
     4021 gtgagcgagc gctcgccacc cgagaggagc gagagccgct tgaccttctt gcccggagga
     4081 cgcgcttcca cctctacgcc ggtggcgagc aggtcgtccg ggtcggtgag cacgagccgg
     4141 ccctcgccgc cggggaacag ccgggcaaac acgtcacgga actcccgctc ggtgtcgcgg
     4201 taggcctcga cgaaggcggc ttcgatgcgc tcgtcgacct cacggacgat ctcgagcagg
     4261 tcgcgacggg tggagcgcag gtcctcgagc tgctcgccga ggaaggcgtg acgctcctcg
     4321 agggcggcgt actcctccag ggcgagcggg ttgaccttgc ccagctgggc cagcgcccgt
     4381 tcggcagcgc gcagccgctt ctcctgctcg gcccgcacga acggcacggg cgccggggcc
     4441 acctcgggct ctgggtcgcc gggcgcgggc gggctcggtg gcaccggctg gtgcgggccg
     4501 tactcgtcga gcagcaccgc ggggtcgacg ccgtactcct cgaccgacct ggcctgcagg
     4561 gtctcgatcc gcagccgttg ctgggcgcgg gccacctcgt cacggtgtgc cgtgtcgacc
     4621 agcgcggaca gcgcctcggc gagctctcgg acctgggtgc ggacctgggc cgcggtggcc
     4681 tcccgctcga ctcgggccgc ctcgaggtcg gtgcgctcgg tggcggctgc ggcgagcgag
     4741 cgctcgagcc ggtcgaggac gagcagggcg ccccggcgga ccgcctcggc ggtggcggcg
     4801 ctgcgtgcca tccgttcgcg gcgggcggcc tcgcgggcac gggcctcgcg ttcgctgcga
     4861 gccgagcgtt ccagcgcctc ggcccgcccg gcggtggccc gcagccgctc ctcgccggtg
     4921 cgcacggcca gacgcgcctc cagctcgcgt gcgcgggcct cggtggcggc ctgcgcgagc
     4981 cggtcgcgtt ccgctccgtc gggctcttcc tggggggcag gggcggcctc ggcgcccgcg
     5041 aggcgctcgt cgagctcggc gctggccgcc cggtcggtcg ccagggcggc ctcggcggcg
     5101 gccgccgcgc gagtcagccg gtcggcctcg cccgccgcag ctcgggcgag cgagcccagc
     5161 tggccgagct gctcggcgac cgcggccagc cgcgcatcag actcgtgcag gcgggacagc
     5221 gcggcatcga cgcgccgctg ggcctgctgg accgcctcac tggcggcttg gagctcgaac
     5281 cgcagtcgct gaccgcgggc cagcgcctcg tcgagccggg tggtggcctc gtcgaccgcc
     5341 gcctggacct cgagcaggct cggggcgctc gccgacccgc ctcgggcacg caggcgaccc
     5401 agcacgtcgc cgtcggtcgt gacgacggtg aggtcgggcc ggcgggccaa cagcgcccgg
     5461 gcctcggcca gcccgccgtc gacgacgacg acgtcgacga gcaggtggtg cagcgccggg
     5521 cgcagcggtt ccggggcctc gaggaggtcg agcaccgcgt gcgcgccggc cggcaccgtg
     5581 ggccggtcga ccatgggcag gtcgttgccg gccgccccga cgaggagccg gacctgaccg
     5641 gcgtcgtcgt ccttgagcag ccgcagcgag tcgacggccg cgtcgaccga gtcgacggcc
     5701 accgcgtcgg cggccgcgcc gagcgcggcg gccaccgccg aggagtagcc cgcctgcacg
     5761 cgcagcagtg cggccaccga gccgagcacc ccggagagcc ggtcgcccgc cgcgagcagc
     5821 acgccggcac cgtcgcggcg ggccagcccg agctcgagcg cctcacggcg ggcggtccat
     5881 gcggtccggt cgcgctcggc ctcccgctcg gcggcccgaa gctcgtcggc ccgcgcgctg
     5941 gcctcgtcga gcgccgcgga ggccgactcg tgctcggcgt cgaggccctc ctcgccctgg
     6001 tcgagcccgg ccacctgcga ctcgagggcg gcgaactcgg cccgggcccg ggtcgcccgc
     6061 tcctcggcct ccgcggctgc cgcgcgcagc cggcccagct cggcctcgcc ggcctccact
     6121 cggctgtgcg ccgctgcgac cttcccggcc aagcgggcca ggccctcgcg gcggtcggcg
     6181 gcggcccggg cggcggccgc catccggcgc tcctcctcgg catgggcggc ctcggcctgc
     6241 ttgcggatgg ccaccgcagc ggccaacgcc tcgccgtcgg cggctacctg ggcggccagt
     6301 cgggcctgct cggcccgggc cgccgcggcc gcggcgtcga gctcgtcggg gtcacgccct
     6361 gcgggcgcct cggccggcgc ctcggtgccg tggcggaccc gctcggcggc cagcgtgacg
     6421 gtgccctgca ggcgctcacg caggccggac aggcggtacc aggtctcctg ggccaccgcg
     6481 agcaccggag cggcctcgcg ggcggccgtc tccagggcct gctcccgcgc ctggccggcg
     6541 gccagcgcgg tctcgagctc ggctcggcga gccttcagcg cggtctcgtc ggcgacctct
     6601 gcctcgagcg tcgcgctcag cgcggccagc tcgtcggcga gcagccgcag ccgggcatcg
     6661 cgtgcgtcgg cctggatgac cgcggcccgt cgggcgacct cggcctgccg gcccagcggc
     6721 ttgagctggc ggcgcagctc ggcggtgagg tcggtgagcc gggtgaggtt ggccgccatc
     6781 gcgtcgagct tgcgcagcgc cttgtccttg cgcttgcggt gcttgaggac cccggcggcc
     6841 tcctcgatga agccgcggcg cacctccggc gtggcggaca gcacggcgtc gagccggccc
     6901 tgcccgacga tgacgtgcat ctcccggccg agcccggagt cggagagcag ctcctggatg
     6961 tcgagcagcc ggcacgccgt cccgttgatc gcgtagtcgg atccgccgtt gcggaacatc
     7021 agccgagtca gcgtcacctc ggcgtagtcg atcggcagcg cgccgtcggt gttgtcgatc
     7081 gtcagggaga cctcggcccg gcccagcggc gcgcgggccg gggtgccggc gaagatgacg
     7141 tcttccatcg tgccgccgcg cagcgacttg gcgccctgct cacccatcac ccaggcgagg
     7201 gcgtcgacga cgttggactt gcccgaccca ttggggccga cgacggcggt gatgcccggc
     7261 tcgaaccgca gcgtggtcgc cgaagcgaac gacttgaagc ccttgagggt gaggctcttc
     7321 agatgcacgc gcgcggactc ctcgccctgc gtcgagcacg cacggcgtca cggggaacag
     7381 gtcgtcggga aaccggacga accataccgg ctgagccggg tcgccgtggc gagattgatc
     7441 agctgtaggc gggcgcctgg ctgggcagcg tgagcagctc gtcggcgtgg gcgtcctgca
     7501 ccgcggcggc caacgcgtcg ttctcccgct ggaggcgacc gagctcgtcc tccaggtcac
     7561 gcacccgctg gcgaagccgc cgcatctcgt cgacgaggcg gaggtcggtg cctccgacgt
     7621 gaccgaggag cgccttggcc atctgtgatc cctccagggg catgtgcaag gccccagcgt
     7681 ctgtcgtctg cgcagccggg cggcacgggt cacggccgtc ttccggtgtc cttagggtcg
     7741 cacccagagt gaccgaaggt caataggtca cgagccggtc acggcctccg acccgacggc
     7801 gctgccccct ccgagccagg tgaggctagg cgaccgggtg gcggggccgc ggttggcagc
     7861 gcgggcagga gtaggacgac cggttcatga acggctcgcg gcgcaccggg gtcgcgcagc
     7921 gtgggcacgg ctggccggcc cgtccataga ccgccagcga gcgctcgaac cacccgctgg
     7981 tcccgttgac gtcgacgtaa agcgcgtcga aggaggtgcc gccggcggcc agcgcctcgg
     8041 tgagcaccgc acgcaccgcc gcgagcaccc gccgcgcgtc gaccgggcgc aaggtggcgg
     8101 tggcgcgggc cccgtgcagc cgggcccgcc agagggactc gtcggcgtag atgttgccca
     8161 ccccggagac cagcgtctgg tcgagcagcg cgcgcttgag cccggtccgg cgcgaccgca
     8221 gcgccgccac gaagccggcg tcgtcgaagt cagggtccaa cgggtcgcgg gcgatgtggg
     8281 ccaccgaggc gggaacgccg tcgacggtcg cgtcgagggc cagcccgccg aacgtgcgct
     8341 ggtcgacgaa tcgcagctgc ggcccccggt cggcgaagcg cagccggacc cgcaggtgcg
     8401 tctcgtccgg cgcgtcatcg gcctcgacca gcagctgccc gctcatgccg aggtgggcga
     8461 ccaacgcctc gccgcggccc gctgtggtct cggggtcgtc gtcgagggtc agccagaggt
     8521 acttgccgcg ccggtgcgcc tggcccagcc gccgcccgga cagccgggcg gcgaagtcgg
     8581 tggcgccggg gacgtgccgg cgtacggcgc ggggatggcg cacctcgacg tcggcgacca
     8641 ctcgtccggc cacccatcgg gccagccccc gccggacgac ctcgacctcg ggcagctccg
     8701 gcactagccg ccgtcgtagg agggcgaccg gccctgagcc ggggcgccga cgacgtcgaa
     8761 ctcggcgcgg atggccgccc acgccgcggc cgcggcgtgc tgctcggcct ccttcttgct
     8821 gcgcccggta cccaccccga agtcacgctc gccggcgcgc acggtggcct ggaacgtctt
     8881 gtcgtggtcg ggaccggagt cgacgacgac gtagtcgggc acgccgagcc ccacgatcgc
     8941 ggtcagctcc tgcaggctgg tcttccagtc gagcccggcg ccgagcgtcg ccgactcggc
     9001 gatcagcggg tcgagcaggc ggtggatgag ctcggaggcg gtctgcagcc cctggtcgat
     9061 gtagaccgca ccgaagagtg cctcgagggt gtcggccagg atcgacgact tgtcgcgtcc
     9121 gccggtggcc tgctcccccc ggcccagctt gatgaaacgc cccagctcga gcgagcggcc
     9181 cacatcggcc agcgcccgca tgttgacgac tgccgcgcgc agcttggcca gctgtccctc
     9241 ggcggtctcg gggttggccc ggaacaacgt gtcggtgacc accagcccga gcaccgcgtc
     9301 gccgaggaac tccagccgct cgttggtcgg cagcccgccg ttctcgtagg cgtaggagcg
     9361 gtgggtcagt gcccggccca gcaacgcctc gggcagcgcc acccccaggc gctcctccag
     9421 ctcagggcag gccgacaccg ccgtcaggcg ccgacgaccg ggcggcgctt gtaggtgccg
     9481 caggtcgggc aggcggtgtg cggcagcttg ggcgcccggc actgctcaca ggtcacgagg
     9541 gtcggcgcgg tggccttcca ctggctgcgg cgggcgcggg tgttggcacg cgacatcttc
     9601 cgcttcggga cggccacggt cagctctctc tctcatcgtc gaacacgctc tgcaacgccg
     9661 cccaccgagg gtcgacgtcg tcgtggtggt gccccgggtc gtcgtcgaga cgagccccgc
     9721 agcgggggca caggcccgaa cagtcgtcac ggcacaccgg ttgcagcggc agcgcgagca
     9781 ccaccgcgtc acgcaggagc ggctcgaggt cgaccaggtc accctcgagc actgccagct
     9841 cggcgtcgtc ggactcacca gccacctctt cgtggtggtg gcgggcctcc tcgtaggcga
     9901 acagctcctg cagctcgacc tccaggtcga cctgcaaggg cccgaggcag cggacgcact
     9961 caccggtggc cactgcctgc gccgtacccg acacgagcac accctcctgc acggcctcca
    10021 gtcgcagcgc gagctcgagg tcgatgccgg cgggcacccc gatcacgtcg ttgcccagac
    10081 cggccggcgc cgcaacggtg cgcagaagtc gccgcatcga ccccggacgt cgaccgagct
    10141 cgcgggtgtc ggccacgagg ggggacgcgg gatccaagcg gtggacgttt ctcctgctct
    10201 gttgggtcat ggcagctctg tgacgaagac gagaccgagg gcaagggtag cggaggccgg
    10261 ggacaggacc aaagcgtcag ggttcgtcga cctcgccgct gcggtggcgg gccaacggct
    10321 cggccagcac cccgagctgg gcgggctcgc ccgcgagcgc accgagctcg tcgagctcat
    10381 gccgcccgtg cagcttttcg cggccgcggc gcaccgccgc caacgtcttg gtcagcacga
    10441 cctcgaagtt ggccagcttg gtgtcgacgt agtcgtcgac ctcgtggcgc atgccgtcgg
    10501 tctcctcgcg ggcactgtcg acgatctcct gcgcctgctg gcgggcctcg gcccagacca
    10561 cgctctcgga gaccagccgg gcctgctcgg cccgggcctg ctcgagcagc cgggccacct
    10621 cgcgccggcc ttcctcgacg accgcgtcgc ggtcggcgag gagcagctgt gcgtggcgga
    10681 actcctcggg cagcagctgg cgcatctcgt cgagcaacgc gagcaggtca ccgcggttga
    10741 cgatgcagga ggccgacagc ggcatcgagc gcgcgttctc gacgagcgcc accagctcgt
    10801 cgagcttgtc gtccacgtcc acgggcggtc tcgtctccag tgtcgtcgtg ctggcgtgcc
    10861 agtcagtgtc gtcgtgtcgt cacgccggtc aggccgggcg ccccagacgc tcacggaggc
    10921 gggcgttgac gacgtcgggg accaggcccg ccacgtcgcc accgagcccg gccacctctt
    10981 tgacgagact agacgacagg taggagtact cggggttggt ggggatgaac aaggtctcga
    11041 ccccggccag ccggtggttc atctgcgcca tcgtgagctc gtagtcgaag tcgctgaccg
    11101 cccgtagccc cttgaggatc acggggatct cacgctcccg gcagaagtcg acgagcaggc
    11161 cgtcgaacgt ctcgatccgc acggccggca ggtccttggt gaccacgccg agcagctcga
    11221 gccgctcgtc gacgtcgaag agcccccgct tggcgatgtt gacgagcacc gccacgacga
    11281 cctcgtcgta gagctcggcg gcccgcgtga tcacgtcgag gtggccgttg gtcaccgggt
    11341 cgaaggaccc agggcagacg catcggcgca gggtgctcac gtcgacctcc ttggttcgcg
    11401 cacgggcagc tgtgaccgcg gtcgggcggg cgcgaccgta ccaaagggtg gcctcgccgt
    11461 agcggcgcgc acgcagcggc tcgaacccgt cgggccactc ccagggctcc cgactggccc
    11521 gctcaaccac caccagggcg ccggcagcga accagcccgc cgccgagagc ccctgcagga
    11581 gcccggccag caccgacgcc gccacggcat agggcgggtc ggcaaagacc aggtcgtacg
    11641 gcgccggggc tggcccctcg gccagtcgct cgacgcggtc gtgacgcaac gtcgccccgg
    11701 gcagggcgag tgtgtcgatg ttggaccgga tggcccgcgc cgccctcgca tcggcctcga
    11761 cgagcaacgc gtggccggca ccccgagaca gcgcctccag accgaccgca ccggagcccg
    11821 cgtaaaggtc gaggacgctg cgtccgtcga gcgggccgag cagcgaccgg accgaggaga
    11881 agagcccctc gcgggcccgg tcggcggtcg gccgggtcga ccggccgggc ggggtggcca
    11941 gccgccgacc acccgcgctg ccggcgatga tccgcgtcac ggtggcgtcc tcaccccttc
    12001 tcgaggtagt cgacccggta ggggtcaaga gtctgctcga tcaccgcgcg caggaccggg
    12061 tgggcctcga gggccgggtc ggcgtcgatg agcgcgccgg cctcctcgcg cgcggcccgg
    12121 atcaccgcct cgtcgcgggt cacccgcagc agccgcaggc tcgagtgggt gcccgactgc
    12181 tcggccccga gcacgtcgcc ctcccgccgc gagcgcaggt cgaccatcga cagctcgaat
    12241 ccgtcgagcg tggcggccac cgcatcgagc cgctcgcggg ccgggctgcc cggctcggcc
    12301 tcggtgacca gcagagccac ggccggtgcc gtgccccggc cgacacgccc gcgcagctgg
    12361 tgcagctgtg agaccccgaa ccggtcggcg tcgaggacca ccatggcggt ggcgttgggc
    12421 acgtcgacgc cgacctcgat gacggtggtg gcgatcagga cgtcgatcgc gccggcagcg
    12481 aagccgcgca tgatcgcgtc cttgtcgtcg gcgggcagcc ggccgtgcag caccgcgagg
    12541 cgaagcccgg cgagcggccc ccgctcgagc tcggcggcca cgtcgacgac cgccttgggt
    12601 ggccgtcgcg cggtgacctc tgccgggtcg gccggcgagt cgggctggtc ctggtcgccc
    12661 tctggcccgg cctcaccgtc atcgccgatg cgggggcaga cgacgtaggc ctggtgcccg
    12721 ccctgcacct cctcccggac ccgcacccag gcgcggtcga ggtagtgcgg cttctccgcc
    12781 gccggcacga cgtgggtcga gatcggggcg cggccgagcg gcagctccgt cagcgtcgcg
    12841 acctcgaggt cgccgaacac cgtcatggcg accgtgcgcg ggatcggggt cgccgtcatg
    12901 accagcacgt gcggaggggt cgcggccttg gcccgcaagg cgtcccgctg ctcgacgccg
    12961 aaccggtgct gctcgtcgac cacgaccagg cccaggtcga ggaagtcgac ccgctcctgc
    13021 agcagcgcat gggtgccgat cacgatgccg gcctggccgg acacggtgtc gagcaacgcg
    13081 gcccgccggt gggcggtcgt ggccgacccg gtgagcaggg cga
//
LOCUS       DATIBB010000196         3441 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate SMAG_U16279
            k141_1445343, whole genome shotgun sequence.
ACCESSION   DATIBB010000196 DATIBB010000000
VERSION     DATIBB010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935774
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3441)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3441)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:28:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,556
            CDSs (total)                      :: 1,536
            Genes (coding)                    :: 1,522
            CDSs (with protein)               :: 1,522
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 4 of 14
            Pseudo Genes (incomplete)         :: 11 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3441
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1445343"
                     /isolate="SMAG_U16279"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..900
                     /locus_tag="VLC50_07765"
     CDS             <1..900
                     /locus_tag="VLC50_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018410036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF27395.1"
                     /translation="NSGGVVIAQVRELVEAGTLDPRRVRVPGVLVDAVVVEPEQRLSF
                     ALRDDPFLTGELRAPATAREPMPMSVRKVIARRAALELRRGDVINLGYGMPDGVAAVL
                     AEEGLDSQVTFTVEQGHIGGVPAGGSDFGMCLNPEASLDAGHQFDWYDGGGLDVAVLS
                     FAQLDAAGNVNVGRFSGRAPGVGGFINISQGAKRLVFIGTFVAGNGAAKLVEEVEQIS
                     FSAQRAVEVGQPVLYVTERAVFRLTPDGLELIELAAGVDLERDVLAHMKFTPKVADPV
                     REIDPRCARPEPLGLTLPDGASA"
     gene            897..1679
                     /locus_tag="VLC50_07770"
     CDS             897..1679
                     /locus_tag="VLC50_07770"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HSF27396.1"
                     /translation="MSTEPVLVTERVGTDGRVLVATLNRPTKGNALNRPLIEALDALA
                     AEVEATCHDAEGPRALVLTGAGAKAFSAGADVHELDGIDGPSAREQMRRGQLAFGRLE
                     RLPVVVIAAVNGFALGGGLELAMAADLRVAAPTARLGQPEITLENLPGWGGTQRLPRL
                     VGRGVATELILTGELISAERALALGLVNRVADDALAAAVALADQIAARNPVAVAGAKR
                     SIRVGLEAGVAAGLEVEADAVAACCETAEQRAAVHAFLHRKR"
     gene            1735..2895
                     /locus_tag="VLC50_07775"
     CDS             1735..2895
                     /locus_tag="VLC50_07775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HSF27397.1"
                     /translation="MSPAEQPTSETGVCSTRTLSIDDALDFAQRQVRALVTAAPGKLT
                     TYTENGRWVFTEDPWAPNWSGGFMAGMMWAFARRTGDPWWREQAEACSRQVEDRKHDA
                     GTHDIGFILEPSWGRWFDWDGSERARDVLIEGGRTMAGRFQPAGGYLCSWVDPGSTFI
                     DIMMNVGIIFRAAALSGDAALHEVALRHSRTSRRYLVRGDGSTLHEGWFDVETGEFLR
                     AATHQGWRSDSDWVRGQAWAIHGFTSAYRHTGDADLLDAARRAADYYIARTPDNGVPP
                     NDWMDPAPAVPYEASAAAIAAAGMLHLANVLGADPAAGRYRAYGLRILETLRSTEFIA
                     ADTEGWQGILRHATYHYRNGLGIDESVMWGEYYFVEALELAAALQEGNGTRG"
     gene            2888..>3441
                     /gene="eda"
                     /locus_tag="VLC50_07780"
     CDS             2888..>3441
                     /gene="eda"
                     /locus_tag="VLC50_07780"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013118225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008675 -
                     2-dehydro-3-deoxy-phosphogluconate aldolase activity
                     [Evidence IEA];
                     GO_function: GO:0008700 - 4-hydroxy-2-oxoglutarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0009084 - glutamine family amino acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HSF27398.1"
                     /translation="MADVTAQLGEHRVVPVVVIDDAARAGAVADALVAGGLPVAEVTF
                     RTAAAAEALRTMATRDDLLVGAGTVITAEQIEQAVAAGARFIVSPGLSRAVVRRAAEL
                     DVPVFPGVATASEVMAALDEGVSTVKFLPAETSGGVAAVKALAGPFPQVRFVPTGGIK
                     AANALDYLALPAVVAVGGSWMVPAA"
BASE COUNT          458 a         1246 c         1270 g          467 t
ORIGIN      
        1 aacagcggcg gcgtcgtcat cgcccaggtc cgcgagctgg tcgaggccgg cacgctggac
       61 ccgcggcggg tgcgggtgcc aggcgtgctc gtcgacgcgg tggtcgtcga gcccgagcag
      121 cggctctcgt tcgcgttgcg cgacgacccg ttcctcaccg gcgagctgcg ggcaccggct
      181 acggctcgtg agccgatgcc gatgtcggtg cgcaaggtga tcgcccgccg ggcggcgctc
      241 gagctgcgcc ggggcgacgt gatcaacctg ggctacggca tgcccgacgg cgtcgccgcg
      301 gtgctggccg aggagggtct cgactcccag gtgacgttca ccgtcgagca gggccacatc
      361 ggcggggtgc ccgccggtgg cagcgacttc gggatgtgcc tcaacccgga ggccagcctc
      421 gacgccgggc accagttcga ctggtacgac ggaggcgggc tcgacgtcgc cgtactcagc
      481 tttgcccagc tcgacgcggc cggcaacgtc aacgtcggtc ggttcagcgg ccgggccccc
      541 ggggtcggcg ggttcatcaa catctcgcag ggcgccaagc ggctggtgtt catcggcacc
      601 ttcgtcgccg gcaacggggc ggccaagctc gtcgaggagg tcgagcagat cagcttttcg
      661 gcccagcgcg cggtcgaggt cggtcagccg gtcctctacg tcaccgagcg cgcggtcttc
      721 cggctcaccc ccgacgggct cgagctcatc gagcttgccg ccggggtcga cctcgagcgc
      781 gacgtcctgg cgcacatgaa gttcaccccc aaggtggccg acccggtccg cgagatcgac
      841 ccacgctgtg cccggcccga gccgctgggc ctgaccctgc ccgacggagc gagcgcatga
      901 gcaccgaacc cgtgctggtc accgagcggg tcggcaccga cgggcgggtg ctggtggcca
      961 cgctcaaccg gcccaccaag ggcaacgcgc tcaaccgtcc gctcatcgag gcgctcgacg
     1021 cgctcgcagc cgaggtcgag gcgacctgcc acgacgccga ggggccgcgt gctctggtgc
     1081 tcaccggcgc cggggccaag gcgttcagcg ccggcgccga cgtccacgag ctcgacggca
     1141 tcgacgggcc cagtgcgcgc gagcagatgc gcaggggcca gctggccttc ggccggctcg
     1201 agcggctgcc ggtcgtggtc atcgccgcgg tcaacgggtt cgccctcggc ggcgggctcg
     1261 agctcgcgat ggccgccgac ctgcgggtcg ccgcaccgac cgcccggctc ggtcagccag
     1321 agatcaccct ggagaacctg cccggctggg gtggcaccca gcggctgcct cggctggtcg
     1381 ggcggggggt ggccaccgag ctgatcctca ccggcgagct catcagcgcc gagcgggccc
     1441 tcgcgctggg cctggtcaac cgggtggccg acgacgcact cgccgcggcc gtcgcgctcg
     1501 ccgaccagat cgccgcccgc aacccggtcg cggtggccgg tgccaagcgc tcgatcaggg
     1561 tcgggctcga ggcgggcgtc gccgccggcc tcgaggtcga ggccgacgcg gtcgcggcct
     1621 gctgcgagac cgccgagcag cgggccgccg tacacgcctt cctgcaccgc aagagatagc
     1681 cgcaccccac cttccccgtc cgcaggcacc acgcaccgag agatgatcat gaacatgagc
     1741 ccggccgagc agcccacgag tgagaccggc gtctgctcga cccgcacgct cagcatcgac
     1801 gacgctctcg acttcgccca gcggcaggtg cgcgccctgg tcacggcggc gcctgggaag
     1861 ctgaccacct acaccgagaa cggccgctgg gtgttcaccg aggacccgtg ggcgcccaac
     1921 tggagcggtg ggttcatggc cggcatgatg tgggccttcg cccggcgcac cggcgacccg
     1981 tggtggcgtg agcaggccga ggcctgcagc cggcaggtcg aggaccgcaa gcacgacgcc
     2041 ggcacccacg acatcgggtt catcctcgag cccagctggg gccgctggtt tgactgggac
     2101 gggtcggagc gggcccgcga tgtgctgatc gagggtgggc gcacgatggc cggccggttc
     2161 cagccggccg gtggctacct gtgctcgtgg gtggacccgg gcagcacgtt catcgacatc
     2221 atgatgaacg tcggcatcat cttccgggcc gcggcgctgt ccggcgacgc ggcgctgcac
     2281 gaggtggctc tgcgccacag ccggaccagc cgccgctacc tggtgcgtgg ggacggcagc
     2341 acgctgcacg agggctggtt cgacgtggag accggcgagt tcctgcgcgc ggccacccac
     2401 cagggctggc gctcggactc cgactgggtg cgcgggcagg cctgggcgat ccacggtttc
     2461 acctcggcct atcggcacac cggcgacgcc gacctgctcg acgccgcgcg gcgggccgcc
     2521 gactactaca tcgcccgcac ccccgacaac ggtgtgccgc ccaacgactg gatggatccg
     2581 gccccggcgg tgccctacga ggcctccgcg gcggccatcg ccgctgccgg gatgctgcat
     2641 ctggccaacg tgctgggcgc cgaccccgcg gccgggcgtt accgcgccta cgggttgcgc
     2701 attctcgaga cgctgcgctc gaccgagttc atcgcggcgg acaccgaggg ctggcaaggg
     2761 atcctgcgcc acgcgaccta ccactaccgc aacgggctgg gcatcgacga gagcgtgatg
     2821 tggggcgagt actacttcgt cgaggcgctt gagctggccg ccgcgctgca ggaaggaaat
     2881 ggcacccgtg gctgacgtga ccgcccagct gggcgagcac cgcgtcgtac ccgtggtcgt
     2941 catcgacgac gccgcgcggg ccggcgcggt cgccgatgcc ctggtcgccg gcgggctgcc
     3001 ggtggccgag gtgaccttcc ggaccgccgc cgcggccgag gcgctgcgca cgatggccac
     3061 ccgcgacgac ctgctcgtcg gtgccggcac ggtgatcacg gccgagcaga tcgagcaggc
     3121 ggtggcggcc ggtgcccggt tcatcgtgtc gccggggctc tctcgggccg tcgtacggcg
     3181 tgccgccgag ctcgacgtac ccgtgtttcc tggggttgcc accgcctccg aggtgatggc
     3241 ggccctcgac gagggcgtga gcacggtgaa gttcctcccg gccgagacct ccggcggggt
     3301 cgcggcggtc aaggcgctgg ccgggccgtt cccgcaggtg cggttcgtgc ccaccggcgg
     3361 catcaaggcc gccaacgcgc tcgactacct ggcgctgccg gcggtcgtgg ccgtcggcgg
     3421 cagctggatg gtgcccgccg c
//
