LOCUS       DATIBQ010000001         7470 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U16294
            k141_166964, whole genome shotgun sequence.
ACCESSION   DATIBQ010000001 DATIBQ010000000
VERSION     DATIBQ010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935789
            Sequence Read Archive: SRR25158331
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 7470)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7470)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:58:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 637
            CDSs (total)                      :: 603
            Genes (coding)                    :: 601
            CDSs (with protein)               :: 601
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7470
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166964"
                     /isolate="SMAG_U16294"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..181
                     /locus_tag="VLB02_00005"
     CDS             <1..181
                     /locus_tag="VLB02_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HSE56462.1"
                     /translation="RLTDKNFGFIKIDGQAKDLFFHANELKNVQWSDLHEGDKVSFEV
                     AEGPKGLNAVNVSKI"
     gene            245..1141
                     /locus_tag="VLB02_00010"
     CDS             245..1141
                     /locus_tag="VLB02_00010"
                     /inference="COORDINATES: protein motif:HMM:NF014600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HSE56463.1"
                     /translation="MKNHLSKKVMVPVLAIIIFLAGLGYGGYRYYRLNRLLHKTEAEL
                     EATTTELTGRVQALEAALTETAAQKTELDSALAAEQGKAATLGEEVVKITDKVQTLEK
                     LTQTDPELLKKYSRVYFLSEHYTPEALSSIDTEYIVPVGRALQFHTKALPFLADLLDD
                     AADDGLALRIASAYRSFQEQKTLKSTYTVTYGTGANKFSADQGYSEHQLGTTVDFATK
                     TIQWASAKFEPTPEYKWLTENAYKYGFVLSYPKNNTYYKFEPWHWRFVGIDLARDLHQ
                     DNKHLYDIDQREIDKYLLTIFD"
     gene            1176..1775
                     /locus_tag="VLB02_00015"
     CDS             1176..1775
                     /locus_tag="VLB02_00015"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HSE56464.1"
                     /translation="MNETQALIQHLGQFSYLGIFGISILANVVVPVPEEVVLLALGYL
                     ARGGRANIFYLIPIVMAGLLVSDIVMYTLSKHNNRLLTRFYNRFFAKRLAGREAWIET
                     HIEKVIFFSRFLIQLRFLGPFIAGQRKVSWKKFLTYELAALVIYVPLVLWAGWYFRSR
                     IDKIVAGIGLVRNGILIAVGITLLISLSKYIRNRTFKAR"
     gene            1837..2190
                     /locus_tag="VLB02_00020"
     CDS             1837..2190
                     /locus_tag="VLB02_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007139698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannose isomerase type II C-terminal
                     cupin domain"
                     /protein_id="HSE56465.1"
                     /translation="MKDGTPYREERPWGSFLQFTHNEPSTVKILTINPGQAFSLQSHQ
                     HRTEQWHVISGTGTIDIGEQEVPVMPGKDYSIPANTLHRITATELPVVVLEIATGDFD
                     ENDITRIDDRYGRAA"
     gene            2141..3310
                     /locus_tag="VLB02_00025"
     CDS             2141..3310
                     /locus_tag="VLB02_00025"
                     /inference="COORDINATES: protein motif:HMM:NF015971.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56466.1"
                     /translation="MKTILLELTTGTVELHSSMFTVVLFLAALAAAGVVISLVLFFKK
                     KQKPRTADIECFNLERSPKNPLISPDQRSYWEAEGTFNPAALLDDDGRIHLLYRAIGS
                     DGMSRLGYASSADGLHFEERLSYPVYESSPGHDIPLEPLGIQEYNPILYPSGGGWSGC
                     EDPRAVIIDNMVYMTYVAFGGWDSIRIALTSLPLADFKRGRWNWKKPVLISDPEMPAK
                     NWVLFPKKINSKYAILKSVVPKIEISYVDSLDFIQSPIKSERLIGPQPGRKNYWDNIM
                     RGAGAPPIYTRYGWLLLYHAIDTKDPDKYKLGAMLLDLKDPTKILHRGPLPLLSPEMH
                     YENDGKPGVIYASGTVVKDDTLYVYYGGGDRHVCVARAPLKKLLSWLIEHGSVEK"
     gene            3342..5240
                     /locus_tag="VLB02_00030"
     CDS             3342..5240
                     /locus_tag="VLB02_00030"
                     /inference="COORDINATES: protein motif:HMM:NF015971.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56467.1"
                     /translation="MFTVRRTEENPLLSPRREHPWEAAATFNWCPVKDGKYTHVVYRA
                     VSERQLLEEPRINRSIIGRATTKDGVHFTDRAPLITPTHDFDRFGCEDPRITKIDDTY
                     YIFYTALSTYPFSAEGIKVAVALSKDLKTVTEKHLVTPFNAKAMALFPEKIDGKYAAL
                     LTVHTDLPGTNVALALFDSIEDMWSETYWKAWHESLDTHIIDLHRGSDDQVEVGAVPI
                     KTKEGWLLIYSHATHYFSSSEHSFGVEAVLLDKKNPRIILGRTKGAFLVPELFYEQTG
                     MVRGIVFPSGALVRGEMLDIYYGGADTHCCTASVRLTDLLQSISPDAPKLVTRFPGNP
                     IITARPNKDWEAHGTFNPAAIDSNGKIHIFYRAMGADDTSTFGLALSTDGFSIEERSN
                     TPVYTPRGGYEEKHHPGNSGCEDPRLVQIKDDIFMTYTAYDGEVPRVALTKISAADFE
                     ARRWKKWSDPVIITPESVPNKDACIIPEPGPEGYLILHRVNESVCADSVISLDFTKEK
                     IKRCIEIISPRRGMWDGRKVGIATPPVKTKTGWLLLYHGVSETGTYRVGAVLLDLKDP
                     TVVLARTAVPLFEPKEDYELKGVVPKVVFPCGIVRRKDKLFIYYGGGDQVVGVATAKL
                     SAILAVLT"
     gene            5237..5992
                     /locus_tag="VLB02_00035"
     CDS             5237..5992
                     /locus_tag="VLB02_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HSE56468.1"
                     /translation="MSNPKGKKVLVFDLDGTLTESKLPVDAEMAALLTNTLTHTTVAV
                     ISGGGLPQFEKQVLPFLALTNEQQKNLFLLPTSGAALFAYEDGALKKIYEEKLTSEEI
                     AHIENTLEKTIDDLKLRPEQTYGALIENRGSQVTYSALGQEARLEKKAVWDPDQEKRK
                     QIVAALAPSLPNFHIGIGGTTSIDITKKGIDKAYGIKKLSEYLHIPITEMAFIGDALF
                     PGGNDEAARASGIKCISVKDVSETKELLKNLSE"
     gene            6066..7016
                     /locus_tag="VLB02_00040"
     CDS             6066..7016
                     /locus_tag="VLB02_00040"
                     /inference="COORDINATES: protein motif:HMM:NF022112.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gmad2 immunoglobulin-like domain-containing
                     protein"
                     /protein_id="HSE56469.1"
                     /translation="MRTFITLLIIVGLGVAAYFLFNRPEPKSYGYITTFQQCATAGFP
                     IMESYPEQCRTPDGRVFTNTAAVPEPGTTTGKENLIVVTTPTPNQEITSPVTISGRAR
                     GNWYFEASFPIEVLDANGKQLGIAPAQAQGEWMTEEFVPFSLTLPFTKPTTETGTIVL
                     HKDNASGEPQFDDELRIPVRFKTAEKTVRLYYYNKAADKDASGNILCSSKGLVTVSRT
                     IPSSQTPIQDTIRLLLKGELTAAEKAHGITTEFPLSGVTLTGASQNGTTLTLSFKDPL
                     HKTSGGSCRVAVLWAQISATAKQFSGIEQVRFLPEELFQP"
     gene            7138..7455
                     /locus_tag="VLB02_00045"
     CDS             7138..7455
                     /locus_tag="VLB02_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HSE56470.1"
                     /translation="MNKKLYVGGISFQASEDDLNNLFASIAQPVSAKIIMDRESGRSR
                     GFGFVEMATEEDAQKAIDQLNGTEQWGRTISVSVAQEKTEGGPRRGGNGGGDRRPFQR
                     RSF"
BASE COUNT         2088 a         1891 c         1693 g         1798 t
ORIGIN      
        1 ccgactcact gataaaaact tcggttttat caaaatcgac ggacaagcaa aagatctttt
       61 cttccacgcg aatgagctca aaaatgtgca atggagcgac cttcacgagg gagacaaggt
      121 aagttttgaa gtagcagaag gtccaaaagg actcaatgcg gtaaacgtta gcaagatcta
      181 gcgcatcaaa aaaccatccc gagggaaact aaggggtggt tttttgtatg ctacaatctc
      241 ggctgtgaaa aaccacctgt ctaaaaaggt tatggtgccc gttctggcaa ttattatctt
      301 tcttgctggt cttggctacg gcggctaccg gtactaccgg ctcaacaggc ttctccataa
      361 aactgaagcc gaactagaag caaccaccac tgaacttacg ggtcgagtac aagcacttga
      421 agctgcactc actgaaaccg ccgctcaaaa aactgaactc gacagcgccc ttgcggccga
      481 acaagggaaa gcggcaacac tcggagaaga agtagtaaaa ataaccgaca aagtgcagac
      541 gcttgaaaaa ctcacccaaa ctgaccccga gctgctgaaa aaatactcaa gggtatactt
      601 tttaagcgag cactacacac cggaagcact gagttcgatt gatacagagt atattgtccc
      661 cgttggacga gcgctccaat ttcataccaa agcgcttccc tttctcgccg acctgttaga
      721 tgacgcagca gatgacgggc tcgccctgcg cattgcttca gcctaccgct cattccaaga
      781 acagaaaacg ttaaaatcaa cctacacagt aacttacgga accggagcaa ataaattctc
      841 tgccgaccaa ggctactcgg agcaccagct gggcactacc gttgacttcg ccaccaaaac
      901 cattcaatgg gcgagtgcga agtttgaacc gacccctgaa tataagtggc tcactgaaaa
      961 tgcctataag tacgggtttg tactctcgta ccccaaaaac aacacctact acaaatttga
     1021 accctggcac tggcgctttg tcggtatcga cctcgcccgt gacctgcacc aagacaacaa
     1081 gcacctctac gacatcgacc aacgcgaaat tgacaagtat ctgctcacta tttttgatta
     1141 atttgtaaac gatttgaacc tgtatactta acttaatgaa cgaaacccag gcactgatac
     1201 agcatcttgg gcagttttca tacctcggaa ttttcggcat ttctattttg gcaaacgttg
     1261 ttgttcccgt tccggaagaa gtagtactgt tggcacttgg ttaccttgcc cgcggcgggc
     1321 gggcaaatat tttttacctc atccctattg tgatggcagg attattggta agtgacatcg
     1381 tcatgtacac cctttcaaaa cataacaacc ggctcctcac tcggttttac aatcgattct
     1441 ttgccaaacg ccttgcgggc agagaagcgt ggattgaaac ccatatcgag aaggttattt
     1501 tcttttcccg cttcttaatt caactccgat ttttgggacc attcattgcc gggcaacgaa
     1561 aagtgtcatg gaaaaagttt ttaacctatg aattagccgc gctcgtcata tacgttccgc
     1621 tggtactctg ggcaggctgg tacttccgca gccgcataga caaaattgtc gctggtattg
     1681 gtttggttcg gaatggtatt ttgattgccg ttggtattac cctcctgatt tcactctcaa
     1741 agtacattcg caatcgaacg tttaaagccc ggtaacagaa gttacgcaca gtgtttgcag
     1801 cactttttta tagtaaactg caaggagtat acaactatga aagacggcac accatatcgc
     1861 gaagagcggc cgtggggaag ttttttgcag ttcacccaca atgaaccatc aacggtgaag
     1921 attctcacta tcaaccctgg tcaggctttt agcttgcagt cgcatcagca tcgcaccgaa
     1981 cagtggcatg ttatttcagg aacaggcacg atagatattg gtgaacaaga agttccagta
     2041 atgcctggca aagactacag cattccagcc aataccctcc accgtattac cgccactgaa
     2101 ctgcctgtcg tggtacttga gattgcaact ggagattttg atgaaaacga tattactaga
     2161 attgacgacc ggtacggtcg agctgcatag cagtatgttt accgtcgtcc tctttcttgc
     2221 agcacttgcc gcggcagggg tagttattag tctagtttta tttttcaaga agaagcaaaa
     2281 accgcgtact gcggacattg aatgtttcaa ccttgagcgt tctccaaaaa atccactcat
     2341 ctcccctgac caaaggagct actgggaagc cgagggcacc ttcaacccag ccgccctgct
     2401 tgatgatgac ggtcgcatac accttttgta ccgcgccatc ggcagcgacg gcatgtcgcg
     2461 cttgggctat gcatcgagcg ctgacggact ccacttcgaa gagcgcctct cctatccggt
     2521 gtatgagtcc tcgcccgggc acgatattcc gctcgaaccg ctcggtattc aagaatataa
     2581 tccgattctc tacccctcgg gtggcggctg gagcgggtgt gaagatccgc gagcagttat
     2641 tatcgataac atggtgtata tgacttacgt cgcttttggc gggtgggatt caatccgcat
     2701 tgcactcacc tcgctccctc ttgccgactt taagcgcgga cgctggaact ggaaaaaacc
     2761 agtacttatt tcggaccccg agatgcctgc taaaaattgg gtactctttc caaaaaaaat
     2821 aaatagcaag tatgcaattc ttaaaagtgt tgtaccaaag atagaaatta gctatgtaga
     2881 tagcttagac tttattcaat cccccataaa aagcgagcgg cttattggtc cgcagccagg
     2941 acgaaaaaat tattgggaca atattatgcg aggcgccgga gcaccgccaa tctacacacg
     3001 ctacggctgg cttttgctgt atcatgcgat cgacacaaaa gatccagata aatacaagct
     3061 cggcgccatg ctccttgatt tgaaagaccc aacaaaaata ttgcatcgcg gaccactccc
     3121 cctactctcc cccgagatgc actacgaaaa tgatgggaaa ccgggtgtta tctacgcatc
     3181 aggaacagta gtgaaagatg acacactcta cgtgtactac ggcggcggcg atcgccacgt
     3241 gtgtgttgct cgagcaccgc tgaaaaaatt gctttcgtgg ctcatcgagc atggttctgt
     3301 agaaaaataa ttttttatta gagtatactt acaattacta catgtttacc gtacgacgca
     3361 cagaagaaaa cccgctcctc tcccctcgtc gcgaacatcc gtgggaagcc gcggcaacat
     3421 ttaactggtg ccccgtaaaa gacggcaagt atacccacgt ggtgtaccgt gctgtttctg
     3481 aacggcaatt gttggaagag ccgaggatta atcgttctat catcgggcgt gccaccacaa
     3541 aagacggtgt tcacttcact gatcgcgctc cgcttattac ccccacccac gacttcgacc
     3601 ggtttggatg tgaagatcca cgcattacca aaattgatga cacctactac attttttata
     3661 ccgcactcag tacttatccc ttttcagctg aaggtattaa ggtagcagtt gcgctcagta
     3721 aagatttaaa aacagtgacc gaaaaacacc tcgttacccc gttcaacgca aaagcaatgg
     3781 cgctctttcc agaaaaaata gacggaaaat acgctgcact gcttacggta cataccgatt
     3841 tacccggcac caatgttgca cttgcgctct ttgattcaat tgaggatatg tggagtgaga
     3901 cctactggaa agcgtggcat gaatcgctcg acacacacat tattgattta caccgcggca
     3961 gcgacgacca ggtagaagtg ggcgcggtgc ctatcaaaac aaaagaagga tggctgctca
     4021 tctactcgca tgcaacacac tacttcagca gttcagagca cagctttggt gttgaagcag
     4081 tactgcttga taaaaagaat ccgcggataa tcctcggaag aaccaaggga gcatttttag
     4141 taccggaact attttatgaa caaacaggaa tggtacgggg tattgtcttc ccctcgggtg
     4201 cactagtacg tggcgagatg ctcgatattt actacggcgg agctgatacc cactgctgca
     4261 cggcaagtgt tcggctcact gatttactgc aaagtattag tcctgatgcg ccaaagctcg
     4321 ttacccgctt cccgggaaat ccgatcatta ctgctcgccc caataaagac tgggaagcac
     4381 acggcacgtt taaccccgct gcaatcgaca gtaacggaaa gatacacatt ttttaccgcg
     4441 ccatgggtgc tgacgacacc tcgacctttg ggcttgctct ttcaactgac ggattctcaa
     4501 ttgaggaacg atcaaatact ccagtgtaca cgccacgggg cggctatgaa gaaaagcacc
     4561 atcccggaaa ctcaggctgc gaagatccgc gattggtaca aattaaagat gacattttta
     4621 tgacctatac cgcctacgat ggcgaggtgc cgcgggtagc actcactaaa ataagcgctg
     4681 ctgattttga ggcacggcgc tggaaaaagt ggagcgaccc ggtcattatc actcccgaga
     4741 gtgtccctaa caaagatgcc tgtattattc ctgaacctgg ccccgaaggg tacttgattt
     4801 tgcaccgcgt taacgagagc gtctgtgccg acagtgttat tagtctcgac ttcaccaaag
     4861 aaaaaataaa acgctgtatt gaaatcattt caccacgccg cggcatgtgg gatggtagaa
     4921 aagttggtat tgccacacca ccagtgaaaa caaaaacagg ttggctgctc ttgtaccacg
     4981 gtgtttctga aaccggcacc tatcgtgttg gcgccgtact cctcgattta aaagatccaa
     5041 ctgttgtact tgcacgaacc gccgtaccgc tctttgaacc aaaagaagat tatgaattaa
     5101 aaggagtagt gccaaaagtg gtcttcccct gcggtatcgt tcgccgtaaa gacaagctat
     5161 ttatttacta cggtggcggc gatcaagtgg taggtgttgc taccgcaaaa ctttctgcaa
     5221 ttttggcagt acttacatga gcaatccgaa aggtaaaaaa gtactcgtat ttgatttgga
     5281 cggcacactc accgagagca agctgccggt tgatgcagag atggcggcgc tgctcactaa
     5341 tacacttaca catactaccg tagcagttat ttcgggcggc gggctgccgc aatttgaaaa
     5401 gcaggttctc ccttttcttg ctctcaccaa cgagcagcaa aaaaatctct ttctactgcc
     5461 cacctcgggc gcagcgctct ttgcatatga agacggagca cttaaaaaaa tatatgaaga
     5521 gaagttgacc agcgaagaaa ttgcccacat agaaaacacc ctcgagaaaa caattgatga
     5581 tttaaaactc cgtcctgaac aaacctatgg tgcactgatt gaaaatcgcg gctcgcaagt
     5641 tacttattcc gccctcgggc aagaggcgcg gcttgaaaaa aaagcagtct gggatccaga
     5701 tcaagaaaag cgcaagcaga ttgttgctgc actcgcaccc tcactcccca acttccacat
     5761 tggcatcggc ggcactacct cgatcgacat tactaagaaa gggatcgaca aagcatacgg
     5821 catcaaaaaa ctctctgagt atctccatat ccccatcacc gagatggcgt tcattggcga
     5881 tgctctcttc cccggcggca acgacgaagc cgcccgtgct tccggcatta aatgtatctc
     5941 tgtcaaagat gtttcggaaa ccaaagaact tcttaagaat ctatctgaat agttttttat
     6001 ttgtaagcaa aaaaactcat tttcgtctat gatagaggta ctagtagcct aaacgagtat
     6061 tttttatgcg aacgtttatt acgttactta ttattgtcgg gcttggagtg gcagcatact
     6121 ttttatttaa ccgccccgag ccaaaaagtt acgggtacat aaccaccttt cagcaatgtg
     6181 ctacggcggg gttccctatt atggagagtt accccgagca gtgcagaaca cccgacggac
     6241 gagtatttac caatactgct gcggtaccgg aaccaggcac taccactgga aaagaaaacc
     6301 ttattgtggt tactacccct accccgaacc aagaaattac aagcccagtt accatcagcg
     6361 gacgcgctcg tggcaactgg tactttgaag catcgttccc tattgaagtg ctcgatgcga
     6421 acggcaagca gcttggtatt gccccagcac aggcacaggg tgagtggatg acagaagagt
     6481 tcgtcccctt ctcgctcacg ctccctttta ccaaaccaac tactgaaaca ggcaccatcg
     6541 tactccacaa agacaacgca agcggtgagc cacagtttga tgatgagctc cgcatccctg
     6601 ttcgctttaa aaccgctgaa aaaacagtgc gactctatta ctataataaa gctgccgata
     6661 aagatgcttc gggaaacatt ctctgcagca gtaaagggtt ggttactgtt tctcgcacta
     6721 tccccagctc acagacacca atccaagaca cgattcgcct gctgttgaag ggtgaactta
     6781 ccgcagctga gaaagcacat ggtatcacaa ctgaattccc actctcggga gttaccctaa
     6841 ccggcgcgag ccagaatggc accacgctca ccctctcatt caaagatccg ctccataaaa
     6901 cgagcggggg cagctgtcgg gtagcggtac tctgggcaca aatttcggca actgcgaagc
     6961 aattttcggg aatagagcag gttcgattct tgcctgaaga gctatttcaa ccctaaccat
     7021 gaggagctaa cttttccaca ggaaaccgag tgttttcttt acaattacgg tttagacggt
     7081 acaatgggta cacctgcgta acgaacccag gatttaacca tttgtatagt aaatttcatg
     7141 aataaaaagt tatacgttgg cggcatttca ttccaagcat ctgaagatga cttgaataac
     7201 ttgttcgcta gcattgctca gccggtttct gcaaaaatca ttatggaccg ggagagcggc
     7261 cgttcacgag gctttggctt cgtggaaatg gcaaccgaag aagatgcaca gaaggcaatt
     7321 gatcaattga acggcaccga acagtggggc cgcacgatca gcgtttcagt agctcaagaa
     7381 aaaaccgaag gcggaccacg ccgcggcgga aacggaggcg gtgatcgtcg ccccttccag
     7441 cgacgctcgt tctagcttgt agaacagttt
//
LOCUS       DATIBQ010000002         8115 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U16294
            k141_661771, whole genome shotgun sequence.
ACCESSION   DATIBQ010000002 DATIBQ010000000
VERSION     DATIBQ010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935789
            Sequence Read Archive: SRR25158331
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 8115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:58:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 637
            CDSs (total)                      :: 603
            Genes (coding)                    :: 601
            CDSs (with protein)               :: 601
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8115
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_661771"
                     /isolate="SMAG_U16294"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..308
                     /locus_tag="VLB02_00050"
     CDS             <1..308
                     /locus_tag="VLB02_00050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56471.1"
                     /translation="PVKRKRVFLTEDLVSTAGSVIKDADIFLSEEATEVFPFCIFSYN
                     TEDVINNLRRANLSLESILTVTDLLPAVKEKLLSNEYELLCKWVDNPKEWFNRHFVS"
     gene            complement(303..1760)
                     /locus_tag="VLB02_00055"
     CDS             complement(303..1760)
                     /locus_tag="VLB02_00055"
                     /inference="COORDINATES: protein motif:HMM:NF015714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HSE56472.1"
                     /translation="MVALLVLIAFSFGIVLGKIVHISALALLVLGVLCLVVFQWNWKK
                     GVIFLLVTVSFFIGMLRMQLAEEQFKNIPLPQKTSITGKVQSVDERAESALIYVQPEG
                     SSTDILIRLYERIPVLPGDTVRSVGKIELPEPFVTDSNRIFDYPNYLKAKNVSFIQNT
                     KSIEVLEAGNGSVARFFAKLNRNTSKLLARTVPYPIDGIVGGMVLGERSSISKNTTNI
                     FQNTGTLHLLVLSGYNITLLVTFLGLLLRRVPFYIRVSMLGVSIISLVLLSGAGIAAV
                     RAGVMGLIAIVGTVSGRQYAALRALGVTFILLLLWNPYLLLSDPGFQLSFLATLCMVA
                     VVPKVEYLFKKIPEKIWKLPLRETVSITVTVSLFMLPFIAYFSGSISLLTIPANFIAA
                     FFVPIITVLGIATIFLSTLVPLLGIIFGTILGFFGNILLFILEAAAKVPQLIVPPFSW
                     WYLVFAYGIIFSVLFWKEIKKPPVFKWGNVKEEIL"
     gene            complement(1777..2499)
                     /locus_tag="VLB02_00060"
     CDS             complement(1777..2499)
                     /locus_tag="VLB02_00060"
                     /inference="COORDINATES: protein motif:HMM:NF012304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase H family protein"
                     /protein_id="HSE56473.1"
                     /translation="MKKELTIYTDGASRGNPGPGGWAAVLLTDDQVFELAGRIARATN
                     NHMELMAVYEALVNKQVHAHRGIVTVLSDSAYVVNGLNSWVYGWEKNGWITSTKTKVE
                     NRELWQSLLKLLRVYGDRLAIQKVSGHSGELYNERCDELAVAAALGKNPKLFSGTLKK
                     YEEFLEETGVTVKQAAPKKKKNDAPAYSYVSLVNGKVHVDKTWGSCEKRVKGAKGARY
                     KKVFSRAEETALVQDYTLSSLL"
     gene            complement(2585..3418)
                     /locus_tag="VLB02_00065"
     CDS             complement(2585..3418)
                     /locus_tag="VLB02_00065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56474.1"
                     /translation="MSHKEFIIIMVLTALITSGVFLASTLITPTVSIPSLFNYSFSST
                     GTLYESGSLLGSTSPYWWVNSGAKLSIDAGTGKTVQGSLPTNDKWRTIYASANPVDTD
                     NGYHPQNIFRLVSKGTWHNFRQQAYFSINQDELSESPNRNASNGLLLFNRYQDSDNLY
                     YTGIRVDGTAVIKKKQAGKYITLAQKPIIAGTYDRAKNPNLLPKSKWIGLRSEVVTTN
                     GVVQIKLYTDIGKTGVWTLAAQTVDDGKTYGPIIDSKSSAGIRTDFMDVAFDDYKIEE
                     L"
     gene            complement(3439..5079)
                     /locus_tag="VLB02_00070"
     CDS             complement(3439..5079)
                     /locus_tag="VLB02_00070"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HSE56475.1"
                     /translation="MYEHIQRTKKLFAISVLSLIMIILFGSFFFEQHEVLDNTLYSFV
                     RAARSASYSFEYRNEKQTLAANALTALSSEGAATGTLAAPGTAAAVPVLLYHGIVNKP
                     DRFSLTVKQFKEQLFALKAAGYHTVHLQDVADFMAGKKVLDDKAFLLTFDDGRTDSYR
                     GADPLLQSLGWSAVMFVATESSIDRAVRSKYYIDEKDTEKMIDSGRWEIGSHAMQYTG
                     GYIPVDAAGTKGNFLSNSMWRTVEGRLETDAEYESRITIELEQSKQILEHTTGTIVNA
                     LAYPFGDYGQQTQNNSRAEDTIADIVKRTYDLAFKQVWSGDDDFSFNYPYDNSMYLRR
                     VEVPTNWSGAELISFLETASPKQLPYDDTFDMETGWKHNWGRVVLQNGTLTLAAPETT
                     TGALTFLDGTGGWSNYFFTVTGAWRDGSTISLLGRYRNGENYTACTFTNENVFINETK
                     NGAQKNLAAAKHSVEMPVDTVSLGMLVDGNAVKCYIGSRLIAYVYTVDQELSSGGIGL
                     KIWDQTPGKSFLVLDALHAVSPEAMQVLLQALPRYVTR"
     gene            complement(5085..6365)
                     /locus_tag="VLB02_00075"
     CDS             complement(5085..6365)
                     /locus_tag="VLB02_00075"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HSE56476.1"
                     /translation="MAIKNGIYRFALIFLGGIVLGLIVYVKSKSVRDISLFLLIYTIF
                     ITTFELSRLISAIFYHRSLRAIRKANEGAAPYEPTVSFVIPCKNEEATIHKTIIKCFE
                     AEYPKEKLEVIVINDGSTDRTGEVLYRMQERYPELKIIDWKVNQGKRHGMTAGFNEAK
                     GEIVVQLDSDSYIVPETFRKLIEPFRNPEIGAVCAHADPENADENLITRMQAGYYFMS
                     FRILKAAESSFMTVFCCSGCSSAYRKSVVLPIMNTWLHEKFLGLPVTWGDDRALTNWV
                     LKLGYRTIYTDDAKAYTICPNSLRVFLKQQVRWKKGWFVNSLFASKFVYRKYPFVGFT
                     YFLPLIIVTLLTPFMAAKAFVYGPLVLGHSPIYYLLGVFLVAVLITIFYRYVAPQNKY
                     WKYVFLWSAINTFLLSFILFYALARLKDRRWGTR"
     gene            complement(6387..7682)
                     /locus_tag="VLB02_00080"
     CDS             complement(6387..7682)
                     /locus_tag="VLB02_00080"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HSE56477.1"
                     /translation="MLKKQNKSKTIAVVGLGYVGLPLALLAEKTYTVIGIDTKREKVE
                     KINARIAPFADEALQKELSGAKKFSATTAFSSAAAVKIIIICVPTPVTVEKMPNYEPV
                     ESACRELGKYLQRGQLVILESTVNPGVCEELVIPILEQVSGLKAGTDFSVAHCPERIN
                     PGDAAWNVSNIPRVVGGFDKRSLKRAVAFYRSILSAEVRPMGSLKEAEAVKIVENSFR
                     NVNIAFVNELAKSFSKMGVDVVNVINGAATKPFAFMAHYPGCGIGGHCIPVDPYYLIA
                     YARKYNGFEHKFLSLACEVNAGMPQYTVDILEKALREKNISIDGATVAVLGLAYKADI
                     DDCRESPSFAIIEELQRRGARVTAYDPHVPKCSDVETLDAALTGKDAVIVAVNHTKFR
                     RLSPNTLKGFGVQVVVDGKNCLPKEQYIQAGLSYQGIGR"
     gene            complement(7821..>8115)
                     /locus_tag="VLB02_00085"
     CDS             complement(7821..>8115)
                     /locus_tag="VLB02_00085"
                     /inference="COORDINATES: protein motif:HMM:NF037628.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56478.1"
                     /translation="TVAACAPYGSGNEKPCFVFEQVTIAAIKQFGKGKEHVELKLEDA
                     TGRTVTAISFFTSVNDFNEPLVAGKQVTLTGTLDQAQGYYGSGIRIRIEHIIV"
BASE COUNT         2231 a         1981 c         1683 g         2220 t
ORIGIN      
        1 ctcccgtaaa aagaaaaagg gtttttctca ccgaagatct tgtttctacc gcagggagtg
       61 ttattaaaga tgccgacatc tttctttcag aggaagcaac cgaggtattc ccgttctgta
      121 ttttttcata caatacagaa gatgtaataa ataacctgag aagggcaaat ctgtctcttg
      181 aatcaattct taccgtaact gatctattgc cagcagtaaa agaaaaactt ttatctaacg
      241 aatacgaact cttatgtaaa tgggtcgata atccaaaaga atggtttaat cgacactttg
      301 tatcataaaa tttcttcttt tacattcccc catttaaaaa ctgggggctt ttttatttcc
      361 ttccaaaata aaacactaaa aataattcca tatgcgaaaa ccaagtacca ccacgaaaat
      421 ggcggaacaa taagttgagg aactttggca gcggcttcaa ggataaacag aagaatgttt
      481 ccaaaaaatc cgaggatagt accgaatata attcctaaca aaggaacaag cgttgaaaga
      541 aagatggtgg caataccaag tacggtaata atgggtacaa aaaatgcagc aatgaagttt
      601 gccggaatag tgagcagtga aatgctgccc gagaagtagg caataaacgg cagcatgaag
      661 agtgagacgg ttacggttat ggaaactgtt tcgcggagcg gcagcttcca tattttttca
      721 ggaatctttt tgaacagata ttctactttc ggtactactg caaccatgca gagtgttgct
      781 aaaaatgaaa gctggaaccc cggatcagaa agcaagaggt acggattcca aagtaataat
      841 aaaataaagg tgacaccaag cgcgcggagt gctgcatact gccgtccact cactgttccc
      901 acaattgcaa tcagtcccat tactcctgcc cgaacagcgg cgatgcctgc ccctgagagc
      961 agcacgagtg aaataatact gacacctaac attgaaactc ggatataaaa cggtactcgc
     1021 cggagcagga gtccgagaaa agttactaga agtgtgatgt tatagcccga gagcaccagc
     1081 aggtgcagtg tgcccgtgtt ctgaaatata ttggttgtat tttttgaaat actactccgt
     1141 tcaccaagca ccatgccgcc gacaatgccg tcaatcggat atggcacagt gcgagcaagt
     1201 aatttgctcg tattgcggtt cagttttgca aaaaatcgag caacgctgcc attccccgct
     1261 tctagcactt caatactttt tgtattttga ataaatgaaa cattttttgc ttttaaatag
     1321 ttcggataat caaatattct gttagaatcg gttacaaatg gttcaggaag ttcaatcttc
     1381 ccgaccgagc gaacagtgtc gccagggagt acaggaattc tttcatacaa acgaatgaga
     1441 atgtctgtac tgctgccttc aggctgtaca tagataagcg cgctctcagc gcgttcatct
     1501 actgattgca ctttccctgt aatagaggtt ttttgtggaa gcggaatatt cttaaactgt
     1561 tcctcggcaa gctgcatccg cagcatacca atgaaaaatg aaaccgtgac caataaaaat
     1621 atgactccct tcttccagtt ccactggaat accaccaagc aaagtacgcc aagcaccagc
     1681 aaagcaagtg ctgagatgtg aactattttc cctaaaacaa ttccgaatga aaatgcgatg
     1741 agaacaagca gggcgaccat acgcaaagca gttattctag agtaatgaag acagtgtgta
     1801 atcctgcacc agcgcagttt cttctgctct tgaaaatact tttttatacc ttgcaccctt
     1861 tgcacctttt acccgttttt cgcacgagcc ccaggttttg tcgacatgca cttttccgtt
     1921 cacgagggaa acataggaat atgctggtgc atcatttttt ttcttctttg gcgcagcctg
     1981 ctttactgtt actcctgttt cctcaagaaa ctcctcatac tttttcaaag tccctgaaaa
     2041 gagctttgga ttctttccga gcgctgctgc taccgcaagt tcatcgcagc gttcgttgta
     2101 gagctcgccc gaatgccctg aaactttttg tatcgccaat ctatcaccat atacacggag
     2161 cagtttgagg agtgactgcc agagctctct attttcaact ttcgttttcg tgctcgttat
     2221 ccagccattt ttctcccacc cgtacaccca agagttaagc ccgtttacca cataggctga
     2281 atctgaaagc actgtaacaa taccgcggtg tgcatgaact tgcttattca cgagcgcttc
     2341 atacactgcc atgagctcca tgtgattatt cgttgcccgc gctatccgcc ccgctaactc
     2401 aaacacctga tcatcagtta ataacaccgc cgcccagccg cctggacccg gattcccccg
     2461 tgatgccccg tcggtatata tagttagttc ttttttcact cgaaaagtat agtatgaacc
     2521 cgacaaagac caaagcaaaa ccgcccctta tgttcgttcg gggcggtttt ttattgcagt
     2581 tatgctagag ctcttctatc ttgtagtcgt caaacgctac atccatgaag tcggtgcgga
     2641 tgccagctga tgatttgctg tcgataatgg gaccatatgt tttcccatca tcaactgtct
     2701 gagcagcaag cgtccagaca cctgttttgc caatgtcagt gtagagctta atctgcacta
     2761 caccgtttgt cgtaactact tcactccgga gtccgatcca cttgcttttt ggaagcaagt
     2821 ttggattctt tgctcggtca taagtaccag caataattgg tttctgagca agggtaatgt
     2881 acttccctgc ctgttttttc ttaatcaccg ccgtaccatc gacccgaatg ccggtgtagt
     2941 agagattatc tgaatcttgg taacgattaa agagcaatag tccattcgat gcattccggt
     3001 ttggactctc actcaattca tcttgattaa tactgaagta cgcctgctgg cggaagttgt
     3061 gccaggtacc cttcgagaca agccgaaaaa tattctgcgg gtggtagccg ttgtcggtat
     3121 ctaccggatt tgccgaagca tatatcgttc gccacttgtc attggtaggc agtgagccct
     3181 gtactgtttt tccggtgccg gcatcgattg aaagttttgc tccggaatta acccaccagt
     3241 agggactggt gctcccgagc aaggatccag attcgtagag cgtccccgtg ctgctgaagg
     3301 aatagttgaa taatgatgga attgatacgg ttggtgttat caaagtactt gcaagaaaca
     3361 cgccgcttgt aataagtgcg gttaaaacca taataataat gaactctttg tgtgacatta
     3421 gtgttataaa acaagcaatt atcgagtcac atatcgcggc aatgcctgca gtagaacttg
     3481 catcgcctca ggagaaacag cgtggagagc gtcgagcacg agaaaacttt tgccaggagt
     3541 ctgatcccat atcttcagcc caataccgcc cgaagatagt tcttggtcta cagtataaac
     3601 gtacgcgatg agccgagagc cgatgtaaca ctttacagca tttccgtcaa caagcatccc
     3661 taaagatact gtatctacag gcatttctac agaatgcttt gcagcagcta gatttttctg
     3721 tgcgccattt tttgtttcat tgatgaatac gttctcattc gtgaacgtac atgcagtgta
     3781 attttcccca tttcgatacc gtccaagcag gcttatggta ctgccatctc gccatgcgcc
     3841 agttaccgta aagaagtaat tactccaccc gcccgtacca tcaagaaatg taagcgctcc
     3901 cgtggttgtt tcaggtgcag caagcgtaag cgttccattt tgcagtacta ccctgcccca
     3961 gttgtgtttc catccggttt ccatgtcaaa ggtatcgtcg tatggcagct gtttcgggct
     4021 ggctgtttct aaaaatgaaa taagttctgc accgctccaa tttgttggca cctcaacacg
     4081 gcgcaagtac atagaattgt cgtatggata gttaaaacta aaatcatcgt ctccactcca
     4141 gacctgtttg aacgctagat cataggtacg ctttacgata tcggcaatag tatcctctgc
     4201 gcgtgaattg ttttgtgttt gctgcccgta atcaccaaac ggatatgcga gtgcatttac
     4261 tatagtgccg gttgtatgct caagtatttg ctttgattgt tcaagttcta tggtaattcg
     4321 actttcatac tcagcgtcgg tttcgaggcg accttcgaca gtgcgccaca tgctattgct
     4381 taaaaagtta ccctttgtac cagctgcatc aaccggaata tacccgccgg tgtactgcat
     4441 ggcatgcgag ccgatttccc accttcctga atcaatcatc ttttcagtat ctttttcatc
     4501 gatgtagtat ttgctgcgta ccgctcgatc aatcgagctt tcagttgcga caaacataac
     4561 cgcgctccac cctaatgatt gtaataatgg atctgcaccg cgatagctgt cggtacgacc
     4621 gtcatcaaat gttaggagaa acgccttatc atcgagtacc ttcttccccg ccataaaatc
     4681 agcaacgtct tgcaagtgaa cagtgtgata cccagcagct ttgagcgcaa acagctgctc
     4741 tttaaattgc tttaccgtca aactaaaacg atctggttta tttacaatac cgtgatagag
     4801 aagcaccggc actgcagcag cagttcccgg tgctgcgagt gttcctgtag cagcaccctc
     4861 tgaagaaagc gctgtaagcg cattagcagc gagtgtctgc ttttcattcc tgtactcaaa
     4921 cgaatacgaa gcagaccgag cagctctgac aaatgagtac agcgtgttat cgagtacttc
     4981 atgctgctca aagaaaaagc tgccgaagag aataatcatt atgagcgata gaacgctgat
     5041 cgcaaataat ttttttgttc tttgtatatg ttcgtacata gtacttagcg tgttccccac
     5101 cggcgatcct taagccgcgc gagcgcgtag aagaggatga aggagaggag aaacgtatta
     5161 attgccgacc acagaaaaac gtacttccag tatttatttt gaggagcaac gtaccgataa
     5221 aaaatagtaa tgagtactgc caccaaaaac acgccgagca gatagtagat cggcgagtgc
     5281 cctagtacca gtggcccgta cacaaacgcc ttggctgcca taaacggggt aagcagcgta
     5341 acaataatga gcggcaaaaa atacgtaaat ccgacgaatg ggtactttcg gtacacaaac
     5401 tttgaggcaa acagagagtt aacaaaccac cccttcttcc accgcacctg ctgctttaag
     5461 aacacccgga gcgaattggg gcaaatggtg tacgcttttg catcatcggt atatatagtt
     5521 cgatacccta acttgagcac ccagttggta agcgcacggt catcacccca cgtaaccggg
     5581 agtcctaaaa atttctcatg cagccaagtg ttcataatgg gcagcaccac gctcttgcga
     5641 tacgccgatg agcagccgct gcagcagaac acggtcataa aacttgattc tgctgctttc
     5701 aaaattctga aagacataaa gtagtaccct gcctgcattc gggttattaa attctcatct
     5761 gcattttcag ggtcagcatg agcgcacacg gctccaattt caggattccg aaacggctca
     5821 ataagttttc gaaacgtctc tggtacaatg tagctgtcac tatcgagctg caccacaatt
     5881 tctcctttcg cttcattgaa cccggctgtc atgccgtgtc gcttcccctg gtttactttc
     5941 caatcaataa tttttagttc tggataccgt tcctgcatcc ggtagagcac ctcgccggtg
     6001 cggtcggtgc tgccatcgtt aataacaatg acttctaatt tttctttcgg atactccgcc
     6061 tcaaaacact tgatgattgt cttatgaatc gtcgcttctt cgtttttaca cggaatcaca
     6121 aaacttaccg tcggctcata gggtgcagcg ccttcatttg cttttcgaat tgcccgcagt
     6181 gagcggtgat agaaaattgc tgaaataagc cgcgaaagct cgaacgtagt aatgaatatc
     6241 gtgtaaataa gcagaaagag cgaaatatcc cgaacactct ttgacttcac gtacacaatt
     6301 aaccccagaa caattcctcc caggaaaata agtgcgaacc gatagatacc atttttgata
     6361 gccatagcaa taactcattc tgtgtattat cgaccgatac cctggtaact taatcctgct
     6421 tggatatact gctctttcgg caaacaattt tttccgtcaa ctaccacctg tacaccaaac
     6481 cctttcaggg tgtttggcga gagtcgtcta aattttgtgt ggttaaccgc aacaatcact
     6541 gcatcttttc cagtaagtgc cgcatcgagc gtttctacat cagaacactt tggcacgtgc
     6601 ggatcatacg ccgttacccg tgctccgcgc cgctgcagct cttcaataat tgcaaacgag
     6661 ggactctcgc ggcagtcatc aatgtctgcc ttatacgcga gcccaagcac tgccacagtt
     6721 gccccatcga ttgaaatatt tttttctcgg agcgcttttt ctaaaatatc aactgtatac
     6781 tggggcatac ccgcgtttac ctcgcaggcg agcgagagaa acttgtgctc gaacccgtta
     6841 tacttgcgcg catacgcaat taagtagtac ggatcaactg gaatgcagtg cccaccaata
     6901 ccgcagcccg ggtagtgcgc cataaacgca aacggtttcg tggcagcacc gttaataacg
     6961 ttcaccacat ccacgcccat ttttgaaaat gattttgcca gctcgttcac aaaagcgatg
     7021 ttcacattgc gaaacgagtt ctcgacaatc tttactgctt ccgcctcctt aagagaaccc
     7081 atcggtcgca cctcggctga aagaatagaa cgataaaaag cgactgctcg tttcaagctc
     7141 cgtttatcaa acccgccaac tacccgtgga atatttgaaa cattccacgc tgcatccccg
     7201 gggttgatgc gttcagggca gtgtgcgaca gaaaaatctg ttcctgcttt taagcccgat
     7261 acttgctcca agattggaat aaccaactct tcacacacgc ccgggttaac cgttgattcc
     7321 aaaatcacca gctgccctcg ctgcaaatac tttcctagtt ctctacatgc agattccacc
     7381 ggttcatagt tcggcatttt ttcaacagtt actggtgtag gaacgcaaat gataataatt
     7441 tttactgcag ctgccgacga aaaagcagtg gttgcactaa attttttcgc acccgaaagc
     7501 tctttttgca gtgcttcatc ggcaaatggt gcaatgcgag cgtttatttt ttcaactttt
     7561 tcacgcttcg tatcaatacc gataacggta tatgtttttt cagcaaggag cgccagcggc
     7621 aagccaacat accctaaacc aacaactgca atagtcttac ttttgttttg ttttttcaac
     7681 atacgtaaaa agaaggaatg gctaaataaa ccaaataaaa ttggctaata atgcactcac
     7741 tgaccttttt tgagcctcta acccgacgaa acagcacgta cctggttacg ttgacaaaaa
     7801 atttttatgt accaaaaaaa ttacacaata atatgttcga ttctgatccg aatcccgctg
     7861 ccgtagtatc cctgcgcctg gtcgagtgtc ccggtaagcg ttacctgctt ccccgctaca
     7921 agcggttcat taaaatcatt gacgctcgta aaaaaagaaa ttgccgtaac cgttcgcccg
     7981 gttgcatcct caagcttcag ctccacgtgt tctttacctt taccaaactg cttaattgcc
     8041 gcaatagtta cctgctcaaa tacaaaacag ggcttttcgt ttcctgaacc gtacggtgcg
     8101 catgctgcta ccgta
//
LOCUS       DATIBQ010000003         5771 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U16294
            k141_2334456, whole genome shotgun sequence.
ACCESSION   DATIBQ010000003 DATIBQ010000000
VERSION     DATIBQ010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935789
            Sequence Read Archive: SRR25158331
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 5771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:58:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 637
            CDSs (total)                      :: 603
            Genes (coding)                    :: 601
            CDSs (with protein)               :: 601
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5771
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2334456"
                     /isolate="SMAG_U16294"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..288
                     /locus_tag="VLB02_00090"
     CDS             <1..288
                     /locus_tag="VLB02_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56479.1"
                     /translation="KEEKVVDPKATWKDVPFYRPKKNDESEDGYKGRVGIHEALNITP
                     AVKEIILRGGSADEVEAQAKKEGMMTMLEDGIFQAAQGKTTTEDVLRVVSE"
     gene            318..1529
                     /locus_tag="VLB02_00095"
     CDS             318..1529
                     /locus_tag="VLB02_00095"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HSE56480.1"
                     /translation="MKFSYKAKAADGTLATGVVEAKDKPEATSKLAAEGKTPIVLTEK
                     EGSFGNISIPFLSNRVKLSEKIVFTKNIAGMLKAGLSLYRALQVLERQTQNRYFKSII
                     TTLIETIDRGSTLSAGIEKYPNVFSSLVVAMVRAGEESGKMPEVLTQIGTQLEKSYAL
                     NKKIKSALMYPGIIVCAIILIGILMFIYVVPTLTKTFKELGTELPTSTKIIIWFSDAL
                     SQHTGALALAVLILFASIYGLMKLASVQRGIDYLITRLPVMGTIVKEMNAARTTRTLS
                     SLLASGVDISRAITITKDVAQNYWYKKVLDQVLVVVQKGEPMSNVFKAETKLYPIMVG
                     EMIEVGEETGTLGTMLLDIALFYEGEVEAKTKDLSTIIEPVLMIFVGAAVGFFAISML
                     TPMYSVMDTIK"
     gene            1637..2518
                     /locus_tag="VLB02_00100"
     CDS             1637..2518
                     /locus_tag="VLB02_00100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56481.1"
                     /translation="MNQKNLLLDAPRPVALSETLSEEKLLWAIDHAYEENGERPVTLF
                     EIQQQFQESTATTFETKFISKLLMLPVETAQRVWGQCHKDTQCALIFQDYGRFNFDLI
                     EKLEIPVRTLTRAFTKQHVLHFGTGEMHDFSSSAEYLLILLKEMKKRDQDLLVPFLKA
                     FGWVRLAEIVFFELEEFGELGEHKQSEEVLGIIQALERREVVVTVSPLFSWLQEHTPE
                     VYSHVWRVLLQSSSFQDLCSSIIVASEDDETELSNNLREVKCTERNGFTLEKAITTAD
                     SVSYADPQQKELYGTGK"
     gene            2550..3536
                     /locus_tag="VLB02_00105"
     CDS             2550..3536
                     /locus_tag="VLB02_00105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56482.1"
                     /translation="MGKLVPYTPPATALVTTKKKPNGEELKNLVCIYKLEKEKQFYSS
                     GKEVTKEMLYRIATLFAVLPYPGNVQSWEEMKRYYREAVGLTALRAALNKLTDSGRAL
                     LNESGEQPLFVNLWQLNVEQFDHASLVTEGAIKERVIALKEWLYIITLLQEQQTPAIR
                     RLFLKEGVPEVLIWDKERVLYALLINQTLGLEQQKWVFTLEDIHRFSQEVQTPQWQVR
                     MQAFLQEGSALFPARTLFAEHSAFLAADPLLQNMFSVARYVPLERDDKALMREATGYA
                     QKKAVGSFYGETKLSETELLRLVGPYRSQLAEYCGLLKKKAQSIPEDNTPPY"
     gene            3592..5388
                     /locus_tag="VLB02_00110"
     CDS             3592..5388
                     /locus_tag="VLB02_00110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56483.1"
                     /translation="MHQKHAGLTLIETLVATAFFLVFSVAIYQLYAQVVTVSNNIRTR
                     TAATQLANEQFELIRNLQYYDVGTVGGIPSGVIDPDQTITRNGITFTIETTIRNIDHP
                     EDGTLGGSPNDLSPADNKFVEVTIACESCKDAVSVTYASWIAPKSLETENGNGALVIR
                     AIDANGQPIAGADVLIENDTLAPPVDITDVTDATGVLTLVDAPPATESYQVTVTKDGY
                     STEQTYTPGAVENPNPSKPHLTVSENAVTQATFTIDETSTFSIDSITDTCTPVGSVAG
                     TFTGTKLIGTTPDVLKQVYSFSTNASGTTTLSNIEWDTYTAAFTSAGYDLIGINPLQP
                     ITVPPGTVQDISLIVQPTNPQTILAAVTDAATGLPLAGATVALSGSGVDETKITNIGS
                     VTQTDWSGSGGAQTYNGSDNYATASSVERTATPGQVTLIDVGGGNYTASGDLTSATFD
                     LGAAATFYQLSWLPTSQPPSAGAAPVRFQIATSATNDVDTIWSFLGPDDTDATYYTTP
                     LEDIAAIHDGKRYLRYKLFLSTENASVTPTVTDISFTYTSGCLPPGQTNFSGLSDGLY
                     TVAASKDGYATDEKTITISGTDASLSFSLPPS"
     gene            5354..>5771
                     /locus_tag="VLB02_00115"
     CDS             5354..>5771
                     /locus_tag="VLB02_00115"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HSE56484.1"
                     /translation="MQASHSLSLRHSGFTIIELLVAVAVASVVGLSIAALARDTVSFN
                     RYYQSSFSVADRAQKLLRPMAEEIRSASQSVAGAYPIDTAGTNEFIFYADIDNDGLVD
                     RVRYYLSGTSLLKDVVAPAGSPLTYTLANAVTTTPVT"
BASE COUNT         1648 a         1281 c         1360 g         1482 t
ORIGIN      
        1 aaagaagaaa aagtagttga cccaaaagca acatggaaag atgtaccatt ttatcgaccg
       61 aaaaaaaatg atgaatctga agatggctac aaagggcgag tgggtattca tgaagcgtta
      121 aatattacac ctgcagttaa agagataatt ttgcgtggtg gttcagccga tgaggttgaa
      181 gcgcaggcaa aaaaagaggg gatgatgacg atgctcgaag acggcatctt ccaagcagca
      241 cagggaaaaa caacgaccga agatgtactg cgcgtggtga gtgagtaata agtttaattt
      301 ggtacactta ctaagtaatg aaattctcat acaaagccaa agcagctgat ggcacgctgg
      361 caacgggggt agtggaagcg aaagataaac ctgaagcgac aagcaaactt gctgcagaag
      421 gaaaaacacc gatcgtactc actgaaaaag agggttcgtt tgggaatatt tcaattcctt
      481 ttttaagcaa tcgggtaaaa ctttctgaaa aaatagtgtt cactaaaaat attgccggca
      541 tgttaaaggc ggggctctcg ctctaccggg cacttcaggt gctcgagcgg caaacgcaga
      601 atcggtactt taaatcaatt attacgactc ttattgaaac aatcgaccgc ggcagcacac
      661 tctcggcagg aattgaaaag tatccaaacg tgttttcatc gctcgtggta gcgatggtgc
      721 gcgccggtga agaatcagga aagatgcccg aggtgctcac ccaaattggc acccaactcg
      781 aaaagagcta cgcgctcaat aaaaaaataa agagcgcact catgtacccc gggattatcg
      841 tctgcgccat tattcttatc ggaattttaa tgtttattta tgtagtaccg acgctcacta
      901 aaacgtttaa agagttgggt acagaactgc caacctcgac caaaattatt atttggtttt
      961 cagatgcact ctcgcagcac actggcgcac tcgctcttgc agtgcttata ctcttcgcaa
     1021 gtatctacgg acttatgaag cttgcttcgg tgcagcgcgg cattgattat ctaatcacac
     1081 ggctcccggt gatgggtacg attgtaaaag aaatgaacgc tgctcgtacg actcggacgc
     1141 tctcatcact cttggcttca ggggtagata tcagccgcgc gattacgatt accaaagatg
     1201 tcgcgcaaaa ctactggtat aaaaaagtgc tcgatcaggt acttgtggta gtgcagaagg
     1261 gcgagccgat gtcgaacgtt tttaaagcag aaacaaaact ctaccccatt atggtaggcg
     1321 agatgataga ggtgggagaa gagacgggta ctcttggcac catgctctta gatattgctc
     1381 ttttttacga aggagaggta gaggcgaaaa cgaaagatct ctcaactatt atcgaaccgg
     1441 tactgatgat ttttgtcggc gctgctgttg gtttcttcgc tatttcaatg ctcacaccaa
     1501 tgtattcggt gatggataca attaagtaga ctacccgccc cactggaggg ctttttgtgt
     1561 gaaaataatg tgattgacaa aaattataga agttgtagat ttaaataaac taaatcttta
     1621 acaaaaacaa cggtatatga accagaaaaa cctactctta gatgctccac gtcctgtggc
     1681 tctttcagaa acactttctg aagaaaagct tttatgggcc attgaccacg cttatgaaga
     1741 aaatggtgag cgaccagtta ctctctttga aatccaacag caatttcaag agtctactgc
     1801 tacgacgttt gaaacaaaat ttattagtaa actactaatg cttccggttg agactgccca
     1861 gcgggtatgg ggacagtgtc ataaagatac acagtgtgca ctcatcttcc aggattatgg
     1921 tcgatttaac tttgacctca ttgagaaatt agagatccct gtaagaacat tgacacgtgc
     1981 gttcactaaa caacatgtgc ttcacttcgg gactggtgaa atgcacgact ttagctctag
     2041 cgctgaatac ctcctcattc ttctaaagga aatgaaaaag agagatcagg atctccttgt
     2101 tccgttcctc aaagcatttg gctgggtacg tcttgccgaa attgtatttt ttgaacttga
     2161 agaatttgga gaacttggag aacataagca gtcagaagaa gtactcggta tcattcaggc
     2221 gcttgaacga agagaggtag tggtaacagt atctccacta ttctcgtggc ttcaggagca
     2281 tacacccgaa gtatactcgc atgtatggcg agtgctgctc cagagcagtt catttcagga
     2341 tctttgcagt agtattatcg ttgccagcga agacgatgag accgagttgt cgaacaacct
     2401 tcgggaggtg aagtgcactg aacgcaacgg attcacactc gagaaggcaa ttactaccgc
     2461 tgattcagtt tcttatgctg atcctcagca gaaagaattg tatggaacag ggaagtaatt
     2521 tctatttagt aaccttaaat cattatggta tgggtaaatt ggtaccatat actccccctg
     2581 ctacggcact ggtgacgaca aagaaaaaac cgaacgggga agagcttaaa aacctagttt
     2641 gtatatacaa actagaaaaa gaaaaacagt tttactcttc aggtaaagaa gtaaccaagg
     2701 agatgctcta cagaattgca actctttttg ctgttcttcc ttaccctgga aatgttcaga
     2761 gttgggaaga aatgaaacgg tattatcggg aagcggtggg gcttaccgca cttcgagcgg
     2821 cgctgaacaa acttaccgac agcggaagag ctcttttgaa tgaatcgggc gagcagcctc
     2881 ttttcgtcaa tttatggcag cttaatgttg agcagtttga ccacgcttct ttagtaacag
     2941 aaggagcaat aaaggagcgg gtgattgctc taaaagagtg gctctatatc attacgctcc
     3001 ttcaagagca acaaaccccc gctatacgac gtttatttct caaagaggga gtaccagaag
     3061 tgctaatctg ggataaggag cgggtattat acgccctgct tataaatcaa acacttggtt
     3121 tagaacagca aaagtgggtg tttacccttg aagatataca ccgcttctca caggaagtac
     3181 aaacaccaca gtggcaagtg cgtatgcaag catttttaca agaaggtagt gcactctttc
     3241 ctgctcgaac cttgtttgca gagcatagtg catttctggc tgcagatcca ctcctccaga
     3301 atatgttttc agtggcacga tatgttccat tagagcgcga tgacaaggct ctcatgagag
     3361 aggcgacagg gtacgctcag aagaaagcag ttggttcttt ttatggagaa actaaactga
     3421 gtgaaacaga gttacttcgt ctggttggtc cataccgatc acaacttgct gagtattgtg
     3481 gtttgctcaa aaaaaaagct caaagcattc cagaagacaa tacacctccc tactaatttt
     3541 agtggggagt tttttttcgt atcgccgtgt tcatgtggga taatagagta catgcatcag
     3601 aaacacgcag gactcacgtt gattgaaaca ctggtggcaa ctgcattttt tttagtattt
     3661 tcagttgcta tctaccagct ctacgcgcag gtggtaacgg tttcaaataa cattcgtacc
     3721 cgcacggctg caacacagct tgccaacgag cagttcgagc ttattcgcaa cttgcagtac
     3781 tatgatgtgg gtacagtcgg cggtattcct tcgggggtga ttgatcccga tcaaacgatc
     3841 acccgaaacg gcataacgtt tacgattgaa acaactattc gcaatatcga ccaccctgaa
     3901 gacggcacgt tgggtggctc accgaatgat ctttctccgg ctgataacaa atttgttgag
     3961 gtaacgattg catgcgaaag ctgcaaggat gcagtgtcgg taacctatgc cagctggatt
     4021 gcgccaaagt cgcttgaaac agaaaacggc aacggtgcct tggtgattcg agcgattgat
     4081 gcaaacgggc agccaattgc gggcgccgat gtgttgattg aaaacgatac acttgctccg
     4141 ccggtcgata tcacagacgt gaccgatgca acaggagtac tcacgcttgt tgatgcgccg
     4201 ccggcaaccg agagttacca ggttaccgtt actaaagacg gatactcaac cgagcagaca
     4261 tacacacccg gcgctgtgga gaatccaaac cccagtaagc cgcacctgac cgtgtctgaa
     4321 aacgcggtaa cccaagcaac gtttactatt gatgaaacaa gcaccttttc tatagattca
     4381 ataaccgata cgtgcacacc ggtggggagt gttgccggca cctttactgg taccaagctt
     4441 attggcacta ctccggatgt gttaaagcag gtgtactcat ttagtactaa cgccagtggt
     4501 actactacac tttcaaatat tgagtgggat acatacactg ctgcattcac cagtgcaggg
     4561 tatgatctca ttggcatcaa cccgctccag ccaattacgg taccgcccgg aacagtgcaa
     4621 gatatttcac tcatagtgca gccgacaaat ccgcagacta ttttggcagc agttaccgat
     4681 gcagcaaccg ggctgccgct cgctggtgcc acggttgctt tgagcggcag tggggtagat
     4741 gaaacaaaaa taacaaacat tggttccgtt actcaaactg attggtcagg ttcaggcgga
     4801 gcacaaacgt ataacggttc agataactat gctactgcca gcagtgttga gagaactgct
     4861 actcccgggc aagtaacact tattgatgtt ggtggcggaa actatactgc ttcgggcgat
     4921 cttacttcgg caacgtttga tttgggcgct gcagcaacct tctaccagct ctcgtggctg
     4981 cctacttcac agccaccatc ggcgggggct gctccggtac gatttcaaat agcaacgagc
     5041 gcaacaaatg atgttgacac tatctggagt tttctgggac ccgatgatac cgatgctacc
     5101 tactacacta ctccgcttga agatattgcc gctatccatg atggcaagcg gtacctgcgc
     5161 tataaactgt ttctctcaac cgagaatgca agcgttactc caaccgttac agatatttca
     5221 tttacctaca cctcgggctg cttaccgccg gggcaaacaa atttttcggg gctctctgac
     5281 ggtctctata cggttgctgc atctaaagat ggatatgcaa ccgatgagaa aaccattaca
     5341 ataagtggta ccgatgcaag cctctcattc tctctccctc cgtcatagcg gattcactat
     5401 tattgaactg ttagtagcag ttgctgttgc tagtgttgtt ggtcttagta tcgcagcgct
     5461 tgctcgtgat accgtttcat ttaatcgcta ctatcagagt tcgttttcag ttgctgatcg
     5521 ggcacaaaaa ctcttgcgcc ccatggctga agaaattcgt tcagcgtctc agtcggttgc
     5581 gggtgcatat ccaattgata ccgcaggaac aaacgaattt attttttatg ccgatattga
     5641 caacgatggt cttgtcgatc gtgttcgcta ctatctctcg ggcacgagct tgcttaaaga
     5701 tgtagtagcg cctgcgggga gcccgctcac ctacaccttg gcaaatgccg taacgacgac
     5761 gccggttacc a
//
LOCUS       DATIBQ010000004         2329 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U16294
            k141_145484, whole genome shotgun sequence.
ACCESSION   DATIBQ010000004 DATIBQ010000000
VERSION     DATIBQ010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935789
            Sequence Read Archive: SRR25158331
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2329)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2329)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:58:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 637
            CDSs (total)                      :: 603
            Genes (coding)                    :: 601
            CDSs (with protein)               :: 601
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2329
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_145484"
                     /isolate="SMAG_U16294"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(244..771)
                     /locus_tag="VLB02_00120"
     CDS             complement(244..771)
                     /locus_tag="VLB02_00120"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HSE56485.1"
                     /translation="MFMHPEAIIAAAQLRPSDVVADFGAGSGFMSRAAAKLVPQGTVY
                     AIELQRGLVERLEHDAKEANLSNLHPIWGDIESPKGSSLADASVDVVILSNILFQLDD
                     KSGAVAEVARVLKPASKVLVIDWTESFGGLGPAPQHVFRPEQARALFEQRGLQVVTDQ
                     LPAGEHHYAILLRKG"
     gene            complement(836..>2329)
                     /locus_tag="VLB02_00125"
     CDS             complement(836..>2329)
                     /locus_tag="VLB02_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HSE56486.1"
                     /translation="KVLNFLYKHTQLETMFHYNMVALVDGIPETLSLKSILTSFIGHR
                     EVVVRRRTAFELKKAKDREHILLGLKKALDHIDRIITLIRGSKDAAAAKLNLIKEFKF
                     SDLQADAILEMRLQKLAGLERKAVELELKEKQELIAELESILSSSKKVFKIIADELRE
                     MQEKYGDERRTKVVKGGVKNISDEDLIPEKDSVLVFTKGGYVKRTDPGEYKMQKRGGV
                     GVVDLETKEEDFVTHLLTTDTHSDLLFFTNLGKAYQIKMYELPEGKRATKGKSIMNFL
                     SLGADEQVTSILAVPKKAKGEAASLLLVTKQGTAKKVTYESFRDVRRSGLLAIRLEAG
                     DELQNVLRVKKGEEIILATAEGQSIRFKESDIREMGRTAAGVRAMKLDKGDVIIGADA
                     ISEEMKNGFLLAMGANGLGKKTALKEYKVQNRGGSGIITAKVTPKTGKLMVAKVVTDA
                     TELVAMSKKGQVIRVDLATIPSTGRQTQGVTVMKLRAGDSLASVTCV"
BASE COUNT          576 a          613 c          474 g          666 t
ORIGIN      
        1 agcaccgcaa aaccaaagat gccgataaat agagcaatga gcgcaatttg aaagttagac
       61 atgctaacta tagtgtacgg tagaacatta aaatttaaaa gaagtgaacc tacatatttt
      121 tttggagctc gcgcaggcgg cgtaagccga gcgcccgccc gactgaaaca gtcgtttcgg
      181 gcggggagca atttgccacg aggcgaatat gcgtgtccaa aaaaagtatg tagggagcga
      241 tatttaccct tttctaagaa gaatagcgta gtgatgctca cccgctggca gctggtcggt
      301 tactacttgt aatccgcgct gttcaaaaag tgctcgtgcc tgctcaggac gaaatacgtg
      361 ctgcggtgct ggtccgagcc cgccaaaact ttccgtccaa tcaatcacta ataccttgct
      421 ggccggcttt aatacacgcg ccacttcagc aactgcgcca cttttatcat caagctgaaa
      481 taaaatattt gaaagtatta ccacatcaac cgatgcatca gccaagcttg aaccttttgg
      541 cgattcaata tcgccccaaa tagggtgtag gttacttaag ttcgcctctt ttgcatcatg
      601 ctctaaacgc tccaccagcc cgcgctgcag ttcaatcgca tacaccgtac cttgcggcac
      661 caattttgct gccgcacgac tcataaatcc actccccgct ccaaaatcag caacaacatc
      721 gcttggtcga agctgcgccg ctgcaataat tgcctcaggg tgcatgaaca taccacacag
      781 tatagttgaa aactatgaag ttataaagga aaaaaatatt tttctccttt cttgattaca
      841 cacaagtaac tgatgcgagt gaatcgcctg cgcgaagctt catcaccgtt actccctgtg
      901 tttgccgccc cgttgaaggg atcgttgcca ggtcaacgcg gatcacctgt cctttttttg
      961 acattgccac gagctcggtt gcatcggtta ctacttttgc taccatcagt ttccccgttt
     1021 ttggcgttac ctttgcggta atgattcctg agccaccgcg gttctgtacc ttgtactctt
     1081 tcaatgcagt tttcttgccg agcccatttg cccccatcgc caacaaaaat ccatttttca
     1141 tttcttcact aatcgcgtcg gcaccaataa ttacatctcc tttatcaagc ttcattgcgc
     1201 gtactcctgc tgcagtgcgc cccatttcgc gaatgtcaga ttctttaaac cggattgatt
     1261 gcccctctgc agttgctaaa ataatttctt cacccttctt tacacgcagc acattttgga
     1321 gttcgtctcc cgcttcaagc cgaatcgcga gcaagccgct ccggcgcaca tctctgaaac
     1381 tctcgtaggt taccttcttt gcggttccct gttttgtaac caacaaaagt gacgctgctt
     1441 cacccttcgc tttctttggt acggcaagaa tcgaggttac ctgttcatca gcgccaagcg
     1501 agaggaagtt catgatcgac ttccccttcg ttgcacgctt tccttctggc agttcgtaca
     1561 ttttaatttg gtacgctttt cctaagttag tgaaaaacag cagatcgctg tgtgtatcgg
     1621 tagtaagcag gtgcgttaca aaatcctctt ccttagtttc taaatcaacc accccaacac
     1681 caccgcgctt ctgcattttg tattcacccg gatcggtacg ttttacatag ccacccttgg
     1741 taaatacgag caccgagtct ttctcgggga taagatcttc atcagaaata tttttcacgc
     1801 cacccttcac tacttttgtc cggcgctcat caccatattt ttcttgcatt tcacggagct
     1861 cgtcggcaat tattttgaaa acttttttcg agctgctcaa aatcgattca agctcggcaa
     1921 tgagctcctg tttttctttc agctccaatt caactgcttt gcgctcgagc ccggcgagtt
     1981 tttgcaggcg catttccaaa atagcatctg cctgcaggtc agagaattta aactctttta
     2041 ttaaattgag ctttgcagca gcagcatctt ttgagccgcg aatcagcgtg atgatgcggt
     2101 caatatggtc gagcgctttt ttaagcccga gcaaaatgtg ctcacgatct tttgccttct
     2161 ttaattcaaa cgccgtgcga cgacgaacca ccacttcacg gtgtccgata aacgaagtaa
     2221 ggattgattt gagtgagagt gtttctggaa taccatcaac gagcgccacc atgttgtagt
     2281 ggaacatcgt ttcaagctga gtatgtttgt ataaaaagtt aagcacctt
//
LOCUS       DATIBQ010000005         8388 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U16294
            k141_1313304, whole genome shotgun sequence.
ACCESSION   DATIBQ010000005 DATIBQ010000000
VERSION     DATIBQ010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935789
            Sequence Read Archive: SRR25158331
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 8388)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8388)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:58:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 637
            CDSs (total)                      :: 603
            Genes (coding)                    :: 601
            CDSs (with protein)               :: 601
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8388
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1313304"
                     /isolate="SMAG_U16294"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..245)
                     /locus_tag="VLB02_00130"
     CDS             complement(<1..245)
                     /locus_tag="VLB02_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HSE56487.1"
                     /translation="MTIIIFILVLLVTVLVHEWGHYFAAKRSGMQVDEFGFGIPPRLF
                     SWKRGLTRFSINALPIGGFVRIAGETGDPRPDVLPGRL"
     gene            complement(247..783)
                     /gene="frr"
                     /locus_tag="VLB02_00135"
     CDS             complement(247..783)
                     /gene="frr"
                     /locus_tag="VLB02_00135"
                     /inference="COORDINATES: protein motif:HMM:TIGR00496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HSE56488.1"
                     /translation="MYSFADFQAELKKVDEWLSKEYSQISTGRAAPALLDGVQVESYG
                     AHQPIKNVASVSIEDPKTLRISPWDKGLIKEIERGIYASNLGFSVSVDDQGIRVIIPQ
                     LTTERRTQLVKLAKDRLEDARIRLRQTREDALEKMKAAKLPEDAEFRAKDELQKLVAG
                     GNQSLEAIFEKKEQEILN"
     gene            complement(791..1408)
                     /locus_tag="VLB02_00140"
     CDS             complement(791..1408)
                     /locus_tag="VLB02_00140"
                     /inference="COORDINATES: protein motif:HMM:NF024575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56489.1"
                     /translation="MLKQIQQFFPQQVFVLKGGSSFFNDFDRELAAAKVRSYCITAPT
                     LLLDHTREILAASAERADEPFYIVCHFETLHGSAHQLLLKTLEEPGENKFYCLITPHP
                     KTLPATILSRAHLLSDVATAEATTSEFAKLPTEAQLAYITKHFGGDAETADKKSHALR
                     LLDELEHVHQKTDPKKISTLYDAKKMLLQANLAPKQVLEFVVTML"
     gene            complement(1603..2625)
                     /locus_tag="VLB02_00145"
     CDS             complement(1603..2625)
                     /locus_tag="VLB02_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HSE56490.1"
                     /translation="MQFPWQKKIGIDLGTANTLVFVSGKGIVLNEPSVVAIARETNKI
                     LAIGSDAKEMLGKTPETVVVHRPLQDGVIADYRITEAMLRHFITKALGGSAWFKPEVL
                     VSVPAGITSTERRAVIEATIRAGAKTAVIVKEPILAAIGAGIPINQARGFMVVDIGGG
                     TTDVAVISLGGIVACTSVKCAGNKIDMAITEHIKRAHGVIVGEQTAEELKINLATALP
                     FSNSKNEMRDVKGRDMVLGMPKTVRVFSNELARAIDAELKLIVKAIKEVLQQTPPELA
                     SDIIDHGIIMTGGTSQLRNLPQLIEEQTGVSVRLADDALFCVAKGSGIALDHLQTYNR
                     SILAKK"
     gene            2701..4566
                     /locus_tag="VLB02_00150"
     CDS             2701..4566
                     /locus_tag="VLB02_00150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56491.1"
                     /translation="MHIVSVIPIQKGIPFDSLTYFSQEPLPQGFLVKVPIQSREVLGT
                     VVSSKPLSDAKLDVKASLFSLKKIAQIIGPGSLDETFLDAAIHTARTSFAPLGALLGE
                     LLPEGALEQRQYITRAAELPKTASHTSLIQGTHDTRLDEYKVLIRETLGHGHSVLLIA
                     PTIAKAEAWHEALVKGIERRTHLLHSECTKTDMKKTYRELFQATKGVLLIATPRFATL
                     PWFTPETLLIDEPSHVGYSTRSRFEIDYREFFTTLGKARGAHIYIGDHLLPFPYIWAG
                     EKKVSTLFPRRYVPEKLQTIDLSGRPFEPLSKELVTLVRHVDATEKSLFIYAQRKGVA
                     PYSVCRTCKTVVVCTQCQTALILKYVNTSNGKERTFICPECGASEPTSYLCAHCGSWD
                     IEAQEIGTDATLDAIRALELRAKLIVIESDRGSDESLREQLASTGPKIIVGTERAFSL
                     LESVAFAAVPHFDRLLSRTALDAGERTLRLLYTLDTLTHDAVILLTKNPKQALIAAIE
                     KKELAPYVAAEGDIARALGYPPFGTILKLAIRVPAAEKERATELVMLAAKPLEAFHVS
                     SKRTRPGASYVTLSFIIKAGKSFFESNLGTYERLLENLGQLPYRAHLENNPKTLF"
     gene            4737..4961
                     /locus_tag="VLB02_00155"
     CDS             4737..4961
                     /locus_tag="VLB02_00155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56492.1"
                     /translation="MAIINVEMKKTGTENPTSLIRRFSRKMQEAGIIQKVKSKRYSER
                     APSALSQKTMALRRIIRRKEAERLKKLGKL"
     gene            4966..5358
                     /gene="ybeY"
                     /locus_tag="VLB02_00160"
     CDS             4966..5358
                     /gene="ybeY"
                     /locus_tag="VLB02_00160"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HSE56493.1"
                     /translation="MQRPSAPLEVIQKPKGKLPRLPFALLRDEILGADYELTIAFISP
                     AAIKKINSEYRKKDYIPNVLSFPLGKKQGEIYACLSQIKKDAPKHGEPYKTFLPFLIL
                     HSMLHLKGYAHGSRMEREEARLREKYNF"
     gene            5391..6548
                     /gene="ftsA"
                     /locus_tag="VLB02_00165"
     CDS             5391..6548
                     /gene="ftsA"
                     /locus_tag="VLB02_00165"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HSE56494.1"
                     /translation="MQKIAVGIDIGTHATRVVVCERDKTSSTLVVLGTGYAEARGMHH
                     GYIVTKAEAAASIEKAVKEAEKSAGIKIKRATIALGGIGLSSEYAIGSAIITRADGVI
                     TTLDIEKAVTEAEGNVELKNKTIIDAHPVGFKIDGKEVPGRPEGVKGLKLEVKVLFIT
                     SFSQHLDDMLDVMQAVGVQVDRIVATPLAASKFVLTDLQRNFGCALVDIGAETVSVAV
                     FENNTITSLHVFGLGSLDITKDIALGLRISPEEAENVKVGAVAFQTFSKKKLDEIIEA
                     RLSDIFELIDKYLKKIGRSGLLPAGAIIIGGGSQMALVETVAKALLRIPAKHGYADIP
                     GVKAPISDQRFLTAYGSTLFGKEHSANDITAPAGIKGILKSIGQFLKQFAP"
     gene            complement(6589..6876)
                     /locus_tag="VLB02_00170"
     CDS             complement(6589..6876)
                     /locus_tag="VLB02_00170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56495.1"
                     /translation="MEIQNPFTKHGFKTCVQKTVDLTLKNDEIAIITLGASSRDFGIH
                     QTLKSDEENKAIDIINKQPAMNDQGEEVIYALCDFRGDIISGSVRYPSFFR"
     gene            6997..8172
                     /gene="ftsZ"
                     /locus_tag="VLB02_00175"
     CDS             6997..8172
                     /gene="ftsZ"
                     /locus_tag="VLB02_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HSE56496.1"
                     /translation="MAKINPDIETAARIKVVGVGGSGANAVNHMINSKVRSVEFIVMN
                     TDVQDLHKSQAEKKIHLGKNLTKGLGAGMNPDIGKRAAEETKAEIQDALKGADMVFIA
                     CGMGGGTGTGGAPIVARAAKELGILTVAVVTKPFFFEGNQRLRIAEAGIDELEKEVDA
                     IIVIPNDRLLAIAGKDMSFKDAFAKCDEVLRQAVEGISDLIASPGLINVDFADMKSVL
                     TDAGTALMGVGSAAGEGRAEKAALQAINSPLLDISINGAKGVLFAIISNDDLTMAEFQ
                     DAAKVITESIDKDARVIIGTIIDDRIKKGELKITVIATGFPSEISKKATLFQGNAIPQ
                     PTIRSIEREERKEAPPSPIVQKAPEPKLPTEDRKPGDDEEDDQDWGAIPAFLRRNKK"
BASE COUNT         2252 a         2083 c         1934 g         2119 t
ORIGIN      
        1 agccgccccg gcagcacatc tggccgcgga tcacctgtct cgcccgcaat gcgcacaaat
       61 ccgccaatcg ggagtgcatt gattgaaaaa cgcgtcagcc cccgcttcca tgaaaatagc
      121 cgcggcggaa tgccgaaccc aaattcatca acctgcatcc ccgagcgctt cgccgcaaag
      181 tagtgccccc actcgtgcac gagcaccgtt actaacagaa cgagtataaa gatgataatg
      241 gtcatattaa tttaaaattt cttgttcttt cttttcaaaa atagcttcaa gtgattggtt
      301 tccgccggca accagttttt gcaattcgtc ttttgcacga aattctgcat cttcaggaag
      361 ttttgccgct ttcatctttt cgagtgcatc ctcgcgtgtc tggcgcaaac gaatgcgcgc
      421 gtcttctaat ctatctttag caagttttac cagctgtgtc cggcgttcgg tagtgagttg
      481 tggaataatc actcggattc cctgatcatc aactgaaacc gaaaatccaa gatttgacgc
      541 gtaaatcccc cgttcaatct cttttatcaa ccccttgtcc cacgggctaa tccggagcgt
      601 ctttggatct tcaattgaaa ccgatgctac gttcttaatc ggctgatgcg caccgtatga
      661 ctcaacctgc accccatcta acaatgccgg cgctgcgcgc ccagtagaaa tttgcgagta
      721 ctctttcgag agccactcat ctaccttttt aagctctgcc tgaaaatctg cgaatgaata
      781 catggggcga ttataacata gtcaccacaa actccagcac ctgttttgga gcgaggtttg
      841 cctggaggag catttttttg gcatcgtaga gggtgcttat tttttttgga tcagtttttt
      901 gatgaacatg ttcaagttcg tcgagtaatc gaagcgcatg gcttttttta tcggcagttt
      961 ctgcatcgcc tccaaagtgc ttagtaatgt aggcaagctg cgcttctgta ggaagttttg
     1021 caaattcaga agttgtcgct tctgctgtgg caacatctga gagcaggtgc gcccgggaga
     1081 gaatcgttgc cggcaatgtt tttgggtgtg gagtaatcaa acagtaaaac ttattctctc
     1141 ccggctcttc gagtgttttt aatagtaatt gatgcgcact gccgtggagt gtttcaaaat
     1201 ggcagacaat gtagaatggt tcatctgccc gctcggcact agccgctaga atctcccggg
     1261 tgtgatcgag caacagtgtt ggagcggtaa tgcagtacga gcgaaccttc gcagccgcaa
     1321 gctcacgatc aaaatcatta aaaaaactac tcccgccctt cagtacaaac acctgctgcg
     1381 gaaaaaattg ttgtatctgc ttaagcatca ctaccatcat atcaccctct cactgagtga
     1441 agattcaaaa ttccagcaac tcgcggaagc tatctgtgca tgattttttg gtacttgttt
     1501 ttgcaaacgc ctcgtttgca tgccaaaaaa acagatagct ggagcggaag aaatcgcctc
     1561 gcagggagat ttctgtttgc tgaaattttg aatcagaacg ttttatttct tcgccaaaat
     1621 tgatcggtta taggtctgca ggtggtcaag agcgataccg ctccccttcg caacacagaa
     1681 gagtgcatca tcggcgagtc gcacactcac cccggtttgc tcctcaatca gctgcggcaa
     1741 gttgcgcagc tgcgaggtgc cgccggtcat aataatgccg tggtcgataa tgtctgaggc
     1801 aagctcgggc ggcgtttgct gtagtacttc tttaattgct ttcacaatta gtttcagctc
     1861 ggcatcaata gcgcgagcaa gctcgtttga aaatacgcgc acggtttttg gcatcccgag
     1921 caccatgtcg cgacctttta catcccgcat ttcgtttttt gagtttgaaa acggaagcgc
     1981 ggttgccaaa ttaattttaa gctcttcagc ggtttgttca ccaacaatta cgccgtgcgc
     2041 tcgcttaatg tgttcagtaa ttgccatatc aattttgttt ccggcacatt ttaccgaggt
     2101 gcaggcaacg atgccgccga gcgaaatcac tgcaacgtcg gtggtgcccc cgccaatatc
     2161 taccaccatg aatccgcgtg cttggttgat aggaatcccc gccccaattg ccgctaaaat
     2221 tggctctttt acgatcactg ccgtttttgc accagctcga atagttgctt caattaccgc
     2281 gcggcgctct gttgaggtaa tccccgccgg tactgaaaca agcacctcgg gcttaaacca
     2341 ggcgcttccg ccgagcgctt tggtaataaa gtggcggagc atcgcctcgg taatgcgata
     2401 gtcggcaatt accccgtctt ggagcggacg gtgcaccacc accgtttcag gcgtcttacc
     2461 gagcatctct ttggcatcgc tcccgattgc taaaatttta ttggtctcac gcgcaatcgc
     2521 tactaccgat ggttcgttta gtacaattcc cttacccgaa acaaacacga gcgtattcgc
     2581 ggttcctaaa tcaataccaa tttttttctg ccagggaaat tgcataaaag tttagtaact
     2641 atagcattta tctaacaccg cttccccctt tacctattcc cctttacact agagctacct
     2701 atgcatatcg tctcggtcat ccctatccag aaaggcatcc ccttcgacag cctcacctac
     2761 ttttctcaag agccgctccc gcaggggttt ttggtgaaag taccgatcca atctcgagaa
     2821 gtactcggta ccgtcgtctc atcaaaaccg ctctcggatg caaagcttga tgtaaaggcg
     2881 tcactctttt cgctcaaaaa aattgcacag attattggtc caggtagttt ggatgaaaca
     2941 ttcttagatg ctgccatcca cactgcccga acatcgtttg caccactagg agcgctgctt
     3001 ggagaactct tgcccgaggg agcgcttgaa cagcggcagt acataacacg cgcagccgag
     3061 ctgccaaaaa cagcatcaca cacttcgctc atacagggca cgcatgatac tcgactcgac
     3121 gagtacaaag tgctcatccg tgagacactc ggccacggac actcagtgct gttgattgct
     3181 cccacgattg caaaggctga agcgtggcac gaggcactag taaaaggaat tgagcgccgc
     3241 acgcacctgc tgcacagcga atgtacaaaa actgatatga aaaagacgta ccgagaatta
     3301 ttccaggcaa ctaagggcgt gctcttgatc gctacccctc ggtttgccac actgccctgg
     3361 tttaccccag aaacactact gattgatgag ccaagccatg tgggctacag tactcgttca
     3421 cgatttgaga ttgactatcg ggaatttttt accactctcg gaaaagcgcg cggtgcacac
     3481 atatacatcg gagatcacct cctgccattt ccgtacatct gggcaggaga gaaaaaagtt
     3541 tccacacttt ttcctcgccg gtatgtgcca gaaaagttac aaacaattga cctctcgggt
     3601 cgacccttcg agccgctttc aaaagaactt gtcacgcttg ttcgtcacgt tgatgcaact
     3661 gaaaaatctc tgtttatata tgcgcagcgc aaaggagttg ctccctattc agtatgccgc
     3721 acttgtaaaa cagtggtggt gtgtacccag tgccaaaccg cgctcatatt aaagtatgta
     3781 aacacctcaa acggaaaaga gcggacattt atttgccccg agtgcggcgc gagcgagccg
     3841 acgagctacc tgtgcgctca ctgcggcagc tgggacattg aggcgcaaga aattggcact
     3901 gatgcaacac tcgatgcgat ccgcgcgctt gagcttcgcg caaagcttat tgttatcgag
     3961 agcgaccgcg ggagtgatga atcactccga gagcagcttg cgagtactgg tccaaaaatt
     4021 attgttggca ccgagcgcgc gttttcgctt ctagagagcg ttgcctttgc tgcagtaccc
     4081 cactttgacc ggctgctgag ccgaacggca cttgatgcgg gcgagcgaac gctccgactg
     4141 ctctacactc tcgatacgct tacacatgac gcagttattt tgctcactaa aaatcccaag
     4201 caggcgctca ttgccgcaat cgaaaaaaaa gaactggcac cgtacgttgc agcagaaggt
     4261 gacatagcgc gtgccttagg ctacccgccc tttggcacca ttcttaaact tgcaattcga
     4321 gttccggctg cagaaaaaga acgcgctaca gagctggtga tgcttgcggc aaaaccgctt
     4381 gaagcatttc atgtaagcag caaacgaact cgccccggcg cctcatacgt taccctgtcg
     4441 ttcatcataa aagcaggaaa gtcattcttt gagagcaact taggtaccta cgagcgcctt
     4501 ttagagaatt tggggcagct tccgtatcga gcgcaccttg aaaataaccc aaaaacttta
     4561 ttttaatcac caatactcaa taaccaaatt tcaaataata agataatagc caataaataa
     4621 tggtctaaat ttggaattta tatattgatt attttggtat tgtttgatta ttggagcttg
     4681 gatattgttt attattcttg ttgctttgcg tgttttttta tggtaacgtt gtgcccatgg
     4741 ctattataaa tgtagagatg aagaaaacgg gcaccgaaaa cccgaccagc ttaattcgcc
     4801 gtttctcgcg caagatgcaa gaagcaggaa ttattcagaa ggtaaaatca aagcgctaca
     4861 gcgagcgcgc tccttcagca ctctctcaaa aaactatggc actccgccga attatccgcc
     4921 gcaaagaggc agagcggctc aagaagctcg gcaaactcta aacgtatgca gcgacccagt
     4981 gcaccgctgg aagtgataca aaaaccaaaa ggcaagctcc cccgcttgcc ttttgccctg
     5041 ctccgtgatg aaattttggg cgccgactac gaactcacca ttgcatttat ctctcctgct
     5101 gcaattaaaa aaataaatag tgagtataga aaaaaagact acataccgaa tgtgctctcg
     5161 tttccgctcg gaaaaaagca gggagaaatt tacgcctgcc tctcgcaaat aaaaaaagat
     5221 gcaccaaaac acggtgaacc gtacaaaact tttttgcctt ttttaattct gcacagcatg
     5281 ctgcatttga aaggatacgc acacggtagt agaatggaga gagaagaagc gcgactgaga
     5341 gagaaatata acttctaaga gcgctctttt ttcttaaccc ttggagaaag atgcaaaaaa
     5401 ttgctgttgg tattgatatc ggcacgcacg caacgcgtgt ggtagtttgc gagcgcgata
     5461 aaacgagcag cacgctcgtc gtgcttggca ccggctacgc tgaagcgcgc ggcatgcacc
     5521 acggctacat tgttaccaaa gctgaagcag cagcaagcat tgaaaaagca gttaaggaag
     5581 cagagaaaag cgcgggtata aaaattaagc gcgccacgat tgcgctcggc ggcatcggac
     5641 tttcatcaga gtacgccatt ggcagcgcta tcatcacccg tgctgacggc gtcattacca
     5701 ctctcgacat tgaaaaggca gttactgaag ctgaaggaaa cgtcgaactt aaaaataaaa
     5761 ctattattga tgcgcacccc gttggcttta agatcgatgg caaagaagtg cccggccgcc
     5821 ccgaaggggt gaaggggttg aagctcgagg taaaagtgct ttttattacg agcttcagcc
     5881 agcacctaga cgacatgctc gatgtgatgc aagcggtggg cgtacaggta gatcgcatcg
     5941 tggctacccc gctcgcggcg agcaagtttg tactgactga tctgcagcgc aactttggct
     6001 gcgcactggt tgatattggt gccgagacag tttcagttgc ggtatttgaa aacaacacca
     6061 tcacttcgct ccacgtgttt gggctcggct cactcgacat taccaaagac attgcgcttg
     6121 ggttgcgcat tagccctgaa gaagctgaga acgtaaaagt aggtgccgtt gcattccaaa
     6181 cattttcaaa aaagaagctc gatgaaatta ttgaagcccg tctctccgac atattcgaac
     6241 tgattgataa gtatttaaaa aagattgggc gaagcggact cttgcctgcc ggtgcaatta
     6301 ttattggcgg cggaagccaa atggcacttg ttgaaacagt ggcaaaagcg ttactgcgca
     6361 ttccagcgaa gcacggctat gctgatatcc cgggcgtgaa agctcctatt tctgatcagc
     6421 ggtttctcac tgcttatggc tctaccctgt ttggtaaaga gcatagtgca aacgatatta
     6481 ccgcgccagc gggcataaaa ggaatactaa aaagcatcgg gcaattccta aaacagttcg
     6541 caccataaaa aattcccgct gttggcggga actaaggcat tcaaataatt agcgaaaaaa
     6601 agaagggtaa cgaacgcttc cagaaataat atctccccga aaatcacata gtgcgtagat
     6661 tacttcttct ccttgatcat tcatagctgg ctgtttgttt atgatatcta tcgctttgtt
     6721 ttcttcatca ctttttagtg tttgatgtat accaaaatct cttgaagaag caccgagagt
     6781 aatgatcgca atttcatcat tttttaaagt aagatcaaca gttttttgaa cacatgtttt
     6841 aaaaccatgt tttgtaaagg ggttttgaat ttccatatct ttatttttaa tagaaagtaa
     6901 tacatatagc gaaattgtca ataacactcc ccttctattc acatttccac cggttctgct
     6961 aagatatgcc cataccactc gggtgggaga gagaacatgg caaaaattaa cccagatata
     7021 gaaaccgctg cgcggatcaa agttgttggt gttggaggct caggcgctaa cgcagtaaac
     7081 cacatgatca attcgaaggt ccgctctgtc gagttcattg tgatgaacac cgacgtgcag
     7141 gatctgcata aatcccaggc tgaaaagaaa attcaccttg gaaaaaacct tactaaaggg
     7201 ctcggtgccg gcatgaatcc cgacatcggc aaacgtgccg ctgaagaaac caaagctgaa
     7261 atccaggatg cactgaaagg tgctgacatg gtgtttattg cctgcgggat gggcggcggc
     7321 accggtaccg gcggtgcccc tattgtggcg cgtgctgcaa aagagcttgg tatcctcacc
     7381 gttgccgtgg tgaccaaacc attcttcttt gaaggcaacc agcgtctgcg gattgctgaa
     7441 gccggcatcg acgagcttga aaaagaagtt gacgcgatta tcgttatccc taacgaccgt
     7501 ctgcttgcaa ttgccggcaa agacatgagc tttaaagacg cattcgcaaa gtgtgatgaa
     7561 gtgctccgtc aggcagttga gggcatctct gacctcatcg cctctccagg gcttatcaac
     7621 gttgacttcg ccgacatgaa gagtgtgctc accgatgcgg gtactgccct tatgggcgta
     7681 ggcagcgctg caggcgaagg tcgtgctgaa aaggcagccc ttcaggcaat caactctccc
     7741 ctgcttgata tctcgatcaa cggcgcgaag ggtgtactct ttgccatcat ttcaaacgat
     7801 gatctcacca tggctgagtt ccaggatgca gcaaaggtaa ttaccgaatc aatcgacaaa
     7861 gacgctcgag tgattatcgg aaccatcatt gatgacagga tcaaaaaagg cgaactcaaa
     7921 atcaccgtta tcgccaccgg cttcccgagc gaaatttcaa aaaaagcaac cctcttccag
     7981 ggcaatgcca tcccccagcc aactattcgc agcattgagc gagaggagcg aaaagaagca
     8041 ccgccatcac caatcgtcca aaaggctccc gagccaaaac tccccactga agatcgaaag
     8101 cccggcgatg atgaggaaga cgaccaagac tggggtgcaa ttcctgcctt tctgcgaaga
     8161 aataaaaaat aatttgcttt taccgattga gtatggtaca atgaaggttc tacataattt
     8221 tctgcgttat tttttctgcg acaaagaggg ttatgaagaa atggttgtga ctataatcca
     8281 ggcttttcca agtctgggtt tttttatttt tcctgacaca atctgctata ctaacccact
     8341 atggaatacg atttagcaat cattgggggc ggaccagcag caactgga
//
LOCUS       DATIBQ010000006         2437 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U16294
            k141_1815403, whole genome shotgun sequence.
ACCESSION   DATIBQ010000006 DATIBQ010000000
VERSION     DATIBQ010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935789
            Sequence Read Archive: SRR25158331
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2437)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:58:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 637
            CDSs (total)                      :: 603
            Genes (coding)                    :: 601
            CDSs (with protein)               :: 601
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2437
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1815403"
                     /isolate="SMAG_U16294"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..537
                     /gene="lexA"
                     /locus_tag="VLB02_00180"
     CDS             <1..537
                     /gene="lexA"
                     /locus_tag="VLB02_00180"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR00498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HSE56497.1"
                     /translation="FYGTKKRMPTYTEMMQLFGFKSKNAVYRLVTKLLDAGLVAKDHL
                     GRLIPTNGFGEIALLGSVKAGFPAEAEQQILDTVNLEDMLVEKKETTYLLTVDGDSMI
                     EAHIASGDLVLAERTNQAKDKDIVIAEVDGEFTMKYFRQQGSKVWLEPANKNFKPIYP
                     TQSLEIRAIVKAVIRKLY"
     gene            736..1209
                     /locus_tag="VLB02_00185"
     CDS             736..1209
                     /locus_tag="VLB02_00185"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HSE56498.1"
                     /translation="MSKSITTIVIIVLLVIIGITVFKKDAVDMAPNTPVTSENTPATG
                     QPAPGTESGTSITGSLEVELGATTKTFTVTAKNFSFIPAQIKVKKGDTVKLTLENSEG
                     MHDLKIDEFNAATKRIGSGQKETITFVASKTGSFEYYCSVGTHRQIGMVGKLIVE"
     gene            1217..1714
                     /locus_tag="VLB02_00190"
     CDS             1217..1714
                     /locus_tag="VLB02_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019941261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HSE56499.1"
                     /translation="MQETKGMIFLKELEAEATATRRCLERVPETLFNWKPHEKSMTLG
                     YLALLIAEIPQWITKMIEDSEIDLATFKHIQPKTTAELVAQFDENQAEAKRALEQLSD
                     EKLTTLFSLKNNGQIIFSSPLDENIGSSINHLVHHRGQLTVYLRLNNIPVPSIYGPSA
                     DDKQF"
     gene            complement(1789..2241)
                     /locus_tag="VLB02_00195"
     CDS             complement(1789..2241)
                     /locus_tag="VLB02_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017482648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HSE56500.1"
                     /translation="METNNIQIQPKQSIITRWQSWAPYFLSVLRIVAAIIFITVGTIK
                     LFAYPIGMPPNGAAVTNWLSLIGIAGMLEVFGGALMLLGLFTRPVAFILAGEMAVAYF
                     IGHASGGFWPVVNGGIDAVLYCFLFLYFSAAGAGPWSIDAKRKKVQVY"
BASE COUNT          816 a          536 c          491 g          594 t
ORIGIN      
        1 ttctacggca ccaaaaagcg gatgcccacc tacaccgaaa tgatgcaact ctttggcttt
       61 aaatcaaaga acgctgtgta tcgcttggta accaaactac ttgatgcagg gcttgttgca
      121 aaagatcacc tggggcgact tatcccaaca aacggttttg gagagattgc actgctcggc
      181 tcagtaaagg caggctttcc ggcagaagct gaacagcaga tacttgatac ggtaaacctt
      241 gaagatatgc tcgtagaaaa aaaagagaca acatatctgc ttaccgtaga tggagactca
      301 atgattgaag ctcatatcgc tagtggtgat ttagtactcg ccgaacgaac aaaccaagca
      361 aaagataaag atatagttat tgctgaagtt gacggtgaat ttacgatgaa gtatttccga
      421 cagcagggaa gtaaggtgtg gctcgaacca gcaaacaaaa actttaaacc catctatcca
      481 acccagagtt tagagattag ggcaattgta aaagcagtaa tacgaaagct gtattaaaaa
      541 tacttaatcc ggtagaaaag aaaaaagccc ccctaggaat agggcggcct ctaacgattg
      601 cttgccatat gaaaaaagag atgtttttac ttttctaggt accatattac acagcttcta
      661 aaaaaatgca agtgggcgac caatttcttc taaaatgcta ttatctgatt agtttatcaa
      721 taagtaaaat atggtatgag taaatcaatc accacaatcg tcattattgt attactagta
      781 atcattggta ttactgtttt taaaaaagat gctgtagata tggcaccgaa tacgcccgtg
      841 acctcggaga acacacccgc aacagggcag cctgctccag gtaccgaatc aggtacctcg
      901 ataacaggtt cactcgaggt agagcttggt gctaccacca aaacatttac agttactgca
      961 aaaaacttct cctttattcc cgcacaaatc aaggtaaaga agggagacac cgtgaagcta
     1021 acacttgaaa acagcgaggg tatgcacgat ctcaaaattg atgaatttaa cgccgcaaca
     1081 aaacgaattg ggagcggtca aaaggaaact attacctttg ttgcaagtaa aacgggtagc
     1141 tttgagtact actgctcggt cggtacccat cgccaaatag gtatggttgg aaagcttatt
     1201 gttgagtaaa tatactatgc aagaaacaaa aggaatgatt ttcttaaaag aactagaagc
     1261 cgaagcaacc gctacccgcc gctgcctaga gcgagttcct gaaacactat ttaattggaa
     1321 accgcatgaa aaatcaatga cgctcgggta cctcgcactt ttgattgcag agattcctca
     1381 gtggatcaca aaaatgattg aagattctga aatagacctt gccaccttca agcatattca
     1441 gccaaaaact actgcggaat tagtcgcaca atttgatgaa aaccaagcag aagcaaaacg
     1501 ggcactagag caactttcgg atgaaaaact taccacactt ttctccttaa aaaataatgg
     1561 gcaaattata ttcagctcac cgctagacga aaacatagga tcatcaatca accacctcgt
     1621 acatcatcgc ggacagctta ccgtgtacct gcgcttaaac aatatccctg taccatctat
     1681 ctatggtcca tccgcagacg ataaacagtt ctaagccgct taacttaagc tcgcaactaa
     1741 aaaacctacc aataataaaa ttggtaggtt ttttgtatac atatagagct agtatacctg
     1801 cactttcttc cgcttcgcat caatactcca tggtcctgca cccgcagctg agaaataaag
     1861 aaaaagaaaa cagtacaaca ccgcatcaat tccgccatta accactggcc aaaaaccgcc
     1921 tgaagcatgg ccaataaagt aggcaacggc catctcgcca gccaaaataa atgcaacagg
     1981 gcgagtaaag agccccaaga gcatgagagc accaccaaac acttcaagca tgccggcaat
     2041 accaataagt gagagccaat tggttactgc agcaccgttg ggtggcatac ctatagggta
     2101 ggcaaagagc tttatagtgc caaccgtaat aaaaattata gcagcaacaa tccgcaggac
     2161 gctcaaaaag taaggcgccc acgattgcca ccgtgtaata atactttgtt ttggctgaat
     2221 ttgaatattg tttgtttcca tatgaacacc attgtaaaac agtttagatg tttagtacaa
     2281 tctacctatg acctacaacg ttcacccgat actggtacat tttccgatag ccttgttagt
     2341 cttatattca gtagtcaaga tcattccatt tcggcgatgg ttccgaaagg ttgcctgggg
     2401 gcatattgaa cgggtgcttt tagtagctgg ggtgctc
//
LOCUS       DATIBQ010000007         2661 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U16294
            k141_1810433, whole genome shotgun sequence.
ACCESSION   DATIBQ010000007 DATIBQ010000000
VERSION     DATIBQ010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935789
            Sequence Read Archive: SRR25158331
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:58:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 637
            CDSs (total)                      :: 603
            Genes (coding)                    :: 601
            CDSs (with protein)               :: 601
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2661
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1810433"
                     /isolate="SMAG_U16294"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            23..478
                     /locus_tag="VLB02_00200"
     CDS             23..478
                     /locus_tag="VLB02_00200"
                     /inference="COORDINATES: protein motif:HMM:NF020977.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HSE56501.1"
                     /translation="MPLHTQIKDGIKEAMKAHDQARLEAFRSVLTACTNELVAKGRQP
                     NEELSDDDVLTVIRRLVKQRKDSIAQFDAAGRADLSSIEREQLSHLEGFLPALLSEDA
                     VREVVLMVKEKLGVTDKQKAGQLVGAVMKELAGKADGGVVKKIVEESFT"
     gene            481..825
                     /locus_tag="VLB02_00205"
     CDS             481..825
                     /locus_tag="VLB02_00205"
                     /inference="COORDINATES: protein motif:HMM:NF015953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HSE56502.1"
                     /translation="MKILIRWLITTLTVFALPYFIEGIAVDGILTAVIVAVCLVFINL
                     IIKPIINLLTLPLNILTLGLFSVVLNGLFFWFVASIISGITVATFTAAVIGAFIISIV
                     NWIVSRFAKDKD"
     gene            827..1864
                     /locus_tag="VLB02_00210"
     CDS             827..1864
                     /locus_tag="VLB02_00210"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HSE56503.1"
                     /translation="MDTHSFEQFRNDFFQLVSESTSVVITAHFSPDDDSIASVLMLYA
                     ILTEKFPEKSVRIIYTGEPVTRYAVFAGFEKIKFVDDVANHLEGVDVLVMLDGSQQHR
                     FSKFPEKLVAVPKTVAIDHHAGVPSTFTLSLVVPKSSSNAELIYHTFLTGDPISKSMA
                     ELILLGIIGDTGGLAHVPADQSSVFSIVKELIEIVGVSIGEFRSRYSTLPQRVFALLQ
                     EFVHNTTYIHVPRWPDAQYTFVKRSVAEMGSYSDEEMSAASHVYLSYYLPRITGYNWG
                     FVISPRSDGECRMSSRSLPKSVNVRVLHERLGIGSGHDRAAGGAFKESDPEECIKKVL
                     EWMKQNEPAIL"
     gene            1869..>2661
                     /gene="obgE"
                     /locus_tag="VLB02_00215"
     CDS             1869..>2661
                     /gene="obgE"
                     /locus_tag="VLB02_00215"
                     /inference="COORDINATES: protein motif:HMM:NF008956.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HSE56504.1"
                     /translation="MAFLDEVTISVSAGRGGDGVVRWRREKFISKGGPAGGDAGRGGD
                     VFVRAIRSLRVLDLYKREQVFKAADGEPGGSRSLHGAGGEDLYLEFPVGTRITNKETG
                     AVIELNKEGQEVAILKGGQGGLGNEHFKSSTNRAPKEATEGKPGETGVFLIEVLLIAD
                     IGLIGFPNAGKSSLLNALTNATAKVGDYPFTTLDPNLGDMFGFIVADIPGLIEGASEG
                     KGLGHKFLKHVTRTKILAHLVSFENQNLMKAYEAVREELGKFDQTL"
BASE COUNT          698 a          540 c          704 g          719 t
ORIGIN      
        1 tatgctggta tactgactct atatgccgct gcatactcaa attaaagacg gaattaaaga
       61 ggcgatgaag gcgcacgatc aggcgcggct cgaggcgttt cggagcgtgc tcactgcctg
      121 cacgaacgag cttgttgcca aagggcggca gccgaacgag gaactctctg atgacgatgt
      181 gctcacggta attcggcggc tcgtgaagca gcggaaagat tcgattgcac agtttgatgc
      241 ggcagggcgt gctgatttgt cgtctattga gcgggagcag ctctcccatc tagaagggtt
      301 tttgccagcg ctcctctcag aagatgccgt gcgtgaggtg gtattgatgg taaaagaaaa
      361 gcttggggta accgacaaac agaaagcagg gcaattggta ggagcggtga tgaaggaact
      421 cgccggcaaa gccgatggcg gcgtcgttaa aaaaatagta gaagaatcat ttacataatt
      481 atgaaaattc tcattcgctg gctcattaca acacttaccg tatttgcact gccgtatttt
      541 attgagggca ttgccgttga cggcatcctt accgcggtta ttgtagcagt gtgtttagtg
      601 ttcattaatt tgattataaa accaatcatt aacttactga cgctgcccct caatattctc
      661 accctcggtt tattttcagt agtgctcaat gggctcttct tttggtttgt tgcttcaatt
      721 attagtggta ttacagtagc aacatttacg gctgcagtta ttggcgcgtt tatcatctct
      781 attgttaatt ggattgtaag ccggtttgca aaagataaag attaatatgg acacgcattc
      841 tttcgagcaa tttcggaacg atttttttca gttagtgtct gaatctacat ctgttgttat
      901 tactgctcat ttttctcctg acgatgattc cattgcatca gtgctgatgc tgtacgcaat
      961 tttaacggaa aaattccctg agaaatctgt ccgaattata tacactggtg aaccagtaac
     1021 tcgatacgca gtgtttgcgg gttttgaaaa aataaaattt gttgatgatg tcgccaatca
     1081 tcttgaagga gttgacgtac tagttatgct cgatggaagc cagcagcatc gtttttccaa
     1141 atttcctgaa aagctagtag cagttccaaa aacggtggca attgatcacc atgcaggggt
     1201 gcctagcaca ttcacacttt cgctggttgt tccaaagagt agttcgaacg ctgaacttat
     1261 ataccacaca tttcttactg gcgatcctat ttcaaagtca atggctgagc ttattctgct
     1321 tggaattatt ggtgatacgg gcgggttagc acatgtgccg gcagatcaat cctctgtatt
     1381 ttcgattgta aaagaattaa ttgaaattgt tggtgtgtcg attggtgagt ttcgctcaag
     1441 atacagtacg ttgccgcagc gagtatttgc gctactgcaa gagtttgtac ataacaccac
     1501 ttatatccac gtaccaaggt ggccagatgc tcagtataca tttgttaaac ggagtgttgc
     1561 tgaaatggga agctactcag atgaagaaat gagtgctgca agccacgtat acttaagcta
     1621 ttatctgcct cgcatcaccg gttacaactg gggctttgtc atttctccgc gttctgatgg
     1681 cgaatgccgg atgtcgagtc gatcacttcc gaaaagtgtg aatgttcggg tgcttcacga
     1741 gcggctcggt attggcagcg ggcatgatag ggcagcgggt ggcgcgttta aagaatcgga
     1801 tcccgaggaa tgcattaaaa aagtcctcga gtggatgaaa cagaacgaac ctgctattct
     1861 gtagcagcat ggcattttta gatgaagtaa caatcagcgt ttcagccggt cgcggcggcg
     1921 atggggtggt gcgctggcgc cgcgagaagt ttatctcgaa aggtggtccg gcaggtggcg
     1981 atgctggccg cgggggcgac gtgtttgtcc gtgccatccg cagtttgcgc gtgctcgacc
     2041 tctacaagcg cgaacaggta tttaaagcag cagatggcga gccggggggc agccgcagcc
     2101 tgcatggtgc cggcggtgaa gatttatacc tcgagtttcc agtaggtact cggattacga
     2161 acaaagaaac aggggcagtg atagagctga ataaagaagg gcaggaggta gcgattttaa
     2221 aaggcgggca gggcggctta ggaaacgagc attttaaaag ctccactaat cgagcaccca
     2281 aagaagcgac cgaaggaaaa ccgggagaaa caggggtatt tttaatcgaa gtactattga
     2341 tcgccgacat cgggctgatt ggttttccaa atgctggaaa gtcgagcctg ttaaacgcgc
     2401 ttactaatgc aactgcaaaa gtaggtgatt atccgtttac gacccttgac ccaaacttag
     2461 gtgacatgtt cggttttatt gttgccgata ttccggggtt gattgaaggc gcgagcgaag
     2521 gcaaaggttt gggacacaaa tttttaaaac acgtaacacg tacaaaaatt ttggcgcacc
     2581 tcgtttcgtt tgagaatcag aatttaatga aggcgtatga ggcggtgcgc gaggagctcg
     2641 gaaaatttga ccaaacacta t
//
LOCUS       DATIBQ010000008         3014 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U16294
            k141_1288160, whole genome shotgun sequence.
ACCESSION   DATIBQ010000008 DATIBQ010000000
VERSION     DATIBQ010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935789
            Sequence Read Archive: SRR25158331
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:58:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 637
            CDSs (total)                      :: 603
            Genes (coding)                    :: 601
            CDSs (with protein)               :: 601
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3014
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1288160"
                     /isolate="SMAG_U16294"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..855
                     /locus_tag="VLB02_00220"
     CDS             <1..855
                     /locus_tag="VLB02_00220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56505.1"
                     /translation="IPENPRPFEQVTATLRSYTIDIDQVVVTWTYSGAVVTSGRGIKS
                     VTVEAGPLGSKRILEATIRTNEGSVVHISTTLVPASTDLLWESIDAYTPPFYKGKPLP
                     ASEGLIRVSAFPERAALARTFSYSWSKDGTVIDTSSGYGKIGFIYKNSYLNSSDAIDV
                     GIRRTDGTVQSGRIQVPTAQPFFVFYRKAGGFTDYATAIGASYRMQDQTETFVAEPFS
                     FSALKPITQVLTLQWSLGGEAVENGDRVNEIVLDRGNGSGSAPLGIAGKHQSFITQFA
                     EASFQLNF"
     gene            859..2457
                     /locus_tag="VLB02_00225"
     CDS             859..2457
                     /locus_tag="VLB02_00225"
                     /inference="COORDINATES: protein motif:HMM:NF014600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HSE56506.1"
                     /translation="MKKIVACILLSFTLFFSFTSTARAVNNFSIVTSTATETTIEVTV
                     KAAAPLEVNGATIYLKQGMQLIQYSTAYNQLAGNNTLKKTFTGLTKATTYEVHVEWMN
                     TSLEKQPPELLGTITTKGTVAAPGRPTVPVAAATPVTEESLTLEALSTSIGAHTTFLG
                     TKTKTYSVVYYLADNNTMLQPTTKQADAIFNTQDSKFYSGATFTGLSPGKTYYVQAEI
                     FDGAVGKKTAVQTAATKPPAIVPSGSTYIPRDTSYDLKGYTVLAPIPGFPTIFPSADN
                     CAAHPEMTYCNFGDFLNLVIELAIALAAVMLVIKLVIHGYSYMTQDVPFIKLQAKTKI
                     GESIIGILLALTAYLLLNTINPRLVNNQVGLQKAVLQTLEEDREDDPGNTIVQDIVSV
                     PKGTVAACKNGIEKVQTKGGVFYACTSIAKNLQTMIDLAYAQNIKLGGGGFRTNEQQK
                     VLRAKHCGGTVNIFNEKAKCVPPTAYPGRSRHESGLAFDFTCERQTIRSKDNKCFVWL
                     KNNAKNYALFNFPKEPWHWSIDGK"
     gene            2476..>3014
                     /locus_tag="VLB02_00230"
     CDS             2476..>3014
                     /locus_tag="VLB02_00230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56507.1"
                     /translation="MRKILFILLIIILAGSVFLYFWTKNTPVAQQNGVQSAVRSFFPV
                     GKDVVEQVTQSSDTPFGNQAINREGVADTTSFEIERGEAAAVSHAPVAGFFLMPTSTI
                     GAAKQEDIVRYAGRESGFVYQVQPGVAPLQITNITLGNVYEALFTNAGNGVVLRFLRE
                     DGRTIGSYYVPIPEADADGV"
BASE COUNT          894 a          707 c          676 g          737 t
ORIGIN      
        1 attcctgaaa atccacgacc attcgagcaa gttactgcaa cactgcgcag ctacactatc
       61 gacatcgatc aggtagtggt tacctggaca tacagcgggg ctgtcgtgac aagcggtcgc
      121 ggtataaaaa gcgttaccgt agaagctggt ccactgggca gtaaacgaat actagaagca
      181 accatccgca ccaatgaagg atcggtggta catatatcaa ctacactggt accggcgagc
      241 accgaccttc tctgggaatc gatcgatgcc tacacaccgc cgttctataa aggaaagccg
      301 ctgcccgcaa gcgaaggact cattcgggtg agtgccttcc ctgagcgagc tgccctggcg
      361 cgaacatttt catacagttg gtcgaaggat ggcaccgtta ttgatacaag ttctgggtat
      421 ggaaaaatag ggtttattta taaaaatagc tatttaaact caagcgatgc tattgatgtg
      481 ggtatccgga gaactgacgg caccgtacag agtggcagaa tacaggttcc taccgcacag
      541 ccattttttg ttttttatcg aaaagcgggt ggatttactg actacgccac cgcgattggc
      601 gcaagctacc gcatgcaaga ccaaaccgaa acatttgttg ctgaaccatt ttcgttctcg
      661 gcactcaaac caatcactca agtacttacc ttgcagtgga gcctcggcgg ggaagcagta
      721 gagaacggcg accgcgtgaa cgaaattgtg ctcgaccgcg gtaatggcag cgggagcgcc
      781 ccgcttggca ttgccggcaa gcatcagagt tttattaccc aatttgctga agcgagcttc
      841 cagctcaatt tttaacgtat gaaaaaaatc gttgcttgca tcctgctcag ttttactctt
      901 ttcttttcat ttacaagcac tgcccgggca gtaaataatt ttagcattgt aacaagcact
      961 gctaccgaaa caaccattga agtaaccgta aaggcagcag caccgcttga agtaaacggc
     1021 gctactatat acctcaaaca gggaatgcag cttatacagt attcaactgc ctataaccag
     1081 cttgcaggaa acaataccct taaaaaaaca tttaccggtc ttaccaaggc aactacgtac
     1141 gaagtgcatg ttgagtggat gaacacctca cttgaaaaac agccgccaga actactcggc
     1201 accataacca ccaaaggaac agttgctgct ccaggacgac ctactgttcc agtggcggcg
     1261 gcaacaccgg tgaccgaaga atcgctcacc cttgaagcac tctctacctc tattggtgcc
     1321 catactactt ttttgggaac aaaaacaaaa acctactcag ttgtttacta tctggctgat
     1381 aacaacacta tgttacagcc aacaacaaaa caagcagatg ctatttttaa cactcaagat
     1441 agtaagttct acagcggagc aacatttacc ggactctctc ctggtaaaac atactacgtc
     1501 caggctgaaa tttttgatgg agcggtaggt aaaaaaactg cagtgcaaac agctgctaca
     1561 aagccgcccg ctatagttcc aagtggctct acgtacatac cacgtgacac cagctacgac
     1621 ctgaaagggt acaccgtact ggcacccatt cctggatttc caaccatttt cccgagcgcc
     1681 gataactgcg ctgcacatcc tgaaatgacc tactgcaact ttggtgattt tttaaacctg
     1741 gttatcgagc tcgctattgc actcgccgca gtgatgctgg taataaaact agttattcac
     1801 ggctacagct acatgaccca agatgtgccg ttcataaaac tccaggcaaa aacaaaaatt
     1861 ggcgagtcga ttattggcat cttgcttgcc cttaccgcgt atctgcttct aaacactatc
     1921 aacccgcgac tcgtaaataa ccaggtagga ttacaaaaag cagtactgca aacactcgaa
     1981 gaagataggg aagatgatcc gggtaacact attgttcagg atattgtttc tgtaccaaaa
     2041 ggaacagttg ctgcgtgtaa aaatggcata gaaaaagtac aaacaaaagg aggagtcttt
     2101 tatgcttgta cctctatcgc aaaaaatctt caaacaatga tcgacctcgc ttatgctcaa
     2161 aatattaagt taggcggagg aggatttaga accaacgagc agcaaaaagt attgcgtgca
     2221 aaacattgtg gtggaactgt aaatattttt aatgaaaaag caaagtgtgt tccgccgact
     2281 gcctatccgg gaagatctcg tcacgagagc gggctcgcat tcgattttac ttgtgagaga
     2341 cagacaatca gatcaaaaga taataagtgt tttgtgtggc tgaaaaataa cgcaaagaac
     2401 tacgcattgt ttaattttcc aaaggagcct tggcattggt cgattgatgg taaataaata
     2461 tagtacaatt actttgtgcg aaaaattctc ttcatacttt taattattat tctcgctgga
     2521 agtgtctttc tctatttttg gacgaagaac acgccagtag cacagcagaa tggggtacag
     2581 agcgcggtgc gctcgttctt tccggttgga aaagatgttg ttgaacaggt aacccaatca
     2641 agtgatacac cattcggaaa ccaagcaata aatagagaag gggttgccga tactacttca
     2701 tttgaaatag agcgcggtga agctgcggca gtgagccatg ctcctgttgc aggatttttt
     2761 ttgatgccca cgagtaccat cggtgctgca aagcaagagg atattgttcg ctacgccggt
     2821 cgtgagagcg gatttgtcta ccaagtacaa cccggtgttg caccgcttca aattaccaac
     2881 atcaccctcg gaaatgtgta tgaagcactc tttactaacg cagggaatgg agtagtactc
     2941 cgctttttgc gtgaagacgg aagaaccatc ggcagctact atgtgcctat tcccgaggct
     3001 gacgcagacg gcgt
//
LOCUS       DATIBQ010000009         2472 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U16294
            k141_1287470, whole genome shotgun sequence.
ACCESSION   DATIBQ010000009 DATIBQ010000000
VERSION     DATIBQ010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935789
            Sequence Read Archive: SRR25158331
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2472)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2472)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:58:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 637
            CDSs (total)                      :: 603
            Genes (coding)                    :: 601
            CDSs (with protein)               :: 601
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2472
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1287470"
                     /isolate="SMAG_U16294"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="VLB02_00235"
     CDS             <1..198
                     /locus_tag="VLB02_00235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56508.1"
                     /translation="TEYIAELEKDESTPEEAPSPTDVIKSIQGQFTTPTAIAPVAREQ
                     QPEKQPESGIKKIDPYREMPE"
     gene            279..680
                     /locus_tag="VLB02_00240"
     CDS             279..680
                     /locus_tag="VLB02_00240"
                     /inference="COORDINATES: protein motif:HMM:NF024078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HSE56509.1"
                     /translation="MRFKVPQFIDIEDKIFGPLTFKQFVYLAGGAGLVYLSFTLLPFF
                     FGALVGIPLALFALALAFYKVNEKPFIHAVEAFFKFHTRSKLYLWQKEQAKKLATATQ
                     AAAIEAQKRSILTESKLKTLSWSLDVIDTRK"
     gene            682..1335
                     /locus_tag="VLB02_00245"
     CDS             682..1335
                     /locus_tag="VLB02_00245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56510.1"
                     /translation="MAIAARPTQDFVQIKEVRDGIVMLLDGSLASVILVSSVNLSLKS
                     YDEQQATLMQFQNFLNSLDFSIQIVIQSRRYDIRPYILTLENRVREQQEPLLRIQTQE
                     YIEFIRTFTEQVNIMRKSFYVVIPYSPAILGGKKGFFANLLPSFGKSGTGDEPLKDFE
                     EQRTQLEQRVGVVEQGLSRLGVRSVQLGTQEIIELFYRTFNPGETTSEAKSVSAPNA"
     gene            1384..>2472
                     /locus_tag="VLB02_00250"
     CDS             1384..>2472
                     /locus_tag="VLB02_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HSE56511.1"
                     /translation="MALFGSKKKPDTNRNLPILPTEMFQSAALELRDIIAPSALEITP
                     KSIHLGDKIARTFFVISYPRYLTDNWFTPIINLDKIFDISIFIHPIETSELLRHFQKK
                     IAEVESQIQGRQQRGLVRDPMLDTAYQDLEELRDNLTQAQEKMFDVGLYITMYANDET
                     ELFRVESEIKSILEARMVYIKPTLFQQEQGFKTVSPLGHDHLNVTQKLNSEPLSSLFP
                     FVSFDLTSETGILYGVNRHNSSLVIFDRFSLENYNSIIFAKSGAGKSYATKLEVIRTL
                     MFDTTVLIIDPEKEYEYLAEAVGGRYFNISLSSDHHINPFDLPIPREDESPGDILRSN
                     IINLVGLFRIMLGGLSAEEDSIIDRAITE"
BASE COUNT          694 a          631 c          529 g          618 t
ORIGIN      
        1 actgagtata ttgccgagct tgaaaaagat gaatcaacac cagaagaagc tccctcacca
       61 accgatgtta tcaagagcat ccaggggcag ttcaccactc caacagcgat tgcaccggta
      121 gctcgcgagc agcaacctga aaaacaaccc gaatcgggga taaaaaaaat cgatccatac
      181 cgagaaatgc ccgagtaatg aactaaaaaa cactgaatgt tcagtgtttt ttagttgtgc
      241 ctatagcatt accagcccga gaacggtaga atacctacat gcgttttaaa gtgccgcagt
      301 ttatcgacat tgaagacaag atttttggac cgcttacctt taagcagttt gtgtacttag
      361 cgggcggggc agggctcgta tacctttcat tcaccctgct gccgtttttc tttggggcac
      421 ttgttggtat accactcgca ctctttgcac tcgcactcgc gttttataag gtaaacgaaa
      481 aaccatttat ccacgccgtt gaggcattct ttaagttcca cacccgctca aagctttact
      541 tatggcagaa agagcaggca aaaaagctcg ctactgctac ccaggcagca gcaatcgagg
      601 cgcagaagcg ttcaatttta accgagagca agttaaagac gctttcgtgg agcttagacg
      661 ttattgatac gagaaaatag tatggcaatt gcagcacgac caacccaaga ttttgttcaa
      721 attaaagaag ttcgcgacgg catcgtgatg ctccttgacg gctcgctcgc ctcggttatt
      781 ttagtgtcat cagtaaacct aagcctaaaa tcatacgacg agcagcaggc aacgctcatg
      841 cagtttcaaa actttctcaa ttcgctcgat ttttcaatac aaattgttat tcaatcgcgc
      901 cggtatgaca ttcgccccta cattctcacc cttgaaaacc gcgttcgcga acagcaagag
      961 ccgctgctgc gcattcaaac ccaagagtac attgagttta ttagaacatt taccgaacag
     1021 gtaaacatta tgcgcaaatc gttttatgtg gttattccgt actcaccggc gattttgggc
     1081 ggaaaaaaag gtttttttgc caatctgctc ccttcatttg gaaagagcgg aacaggggat
     1141 gaaccgctca aagattttga agagcagcgc acccagctcg agcagcgagt gggtgtggta
     1201 gagcagggac tctcgcgctt gggagtacga agcgtacagc tggggacgca ggaaattatt
     1261 gaactcttct atcgtacctt caaccccgga gaaacaacca gtgaagcaaa gagtgtgtcg
     1321 gcgccaaacg cgtagaccct aatactatct acttgccgct ctcttgctat actacgtaca
     1381 cgtatggctt tatttggctc caagaaaaaa cctgacacaa acagaaactt gccgattctc
     1441 ccgactgaaa tgtttcaatc agcagcgctc gagctccgcg atattattgc gccctcagcg
     1501 cttgaaatta cccccaaatc aatccacctg ggcgataaaa tcgcccgcac cttctttgtt
     1561 atctcgtacc cacgctacct caccgacaac tggttcaccc cgatcatcaa cctcgataaa
     1621 atattcgata tttctatttt tattcacccg attgaaacat ccgagctctt gcggcacttt
     1681 caaaaaaaga ttgctgaggt agagagtcaa attcaaggac gacagcagcg agggctggtg
     1741 cgcgacccca tgctcgatac tgcctaccaa gatttagagg agctccgcga caacctcacc
     1801 caggcacaag aaaaaatgtt tgacgtgggg ctctacataa ccatgtatgc caacgatgag
     1861 accgagcttt tccgagtgga gagtgagatt aaatcaattc ttgaagcgcg gatggtctac
     1921 atcaaaccaa ctcttttcca gcaagagcaa gggtttaaaa ccgtttcacc gcttgggcac
     1981 gaccacttaa acgtaacaca aaagctcaac tccgagccgc tctcgagttt gtttccgttc
     2041 gtctcgttcg acctcacttc agaaacgggc attttgtacg gcgttaaccg gcacaattcc
     2101 agcctcgtta ttttcgaccg ctttagtttg gaaaactaca attcgattat ctttgcaaaa
     2161 tccggtgccg gaaaatcata cgcgaccaaa ctcgaggtca tccgcacctt gatgttcgac
     2221 accactgtcc ttattatcga tcctgaaaaa gagtacgaat acttggctga agcggtgggg
     2281 ggacgctatt ttaatatttc actctcctct gaccaccaca tcaacccgtt tgatctgcca
     2341 attccacgtg aagacgaatc gccgggcgac attttacgat caaatatcat taacttggtt
     2401 gggctattcc gcatcatgtt gggtggactt tctgctgaag aagactcgat tatcgaccgc
     2461 gccattactg aa
//
LOCUS       DATIBQ010000010         4870 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U16294
            k141_283752, whole genome shotgun sequence.
ACCESSION   DATIBQ010000010 DATIBQ010000000
VERSION     DATIBQ010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935789
            Sequence Read Archive: SRR25158331
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 4870)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4870)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:58:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 637
            CDSs (total)                      :: 603
            Genes (coding)                    :: 601
            CDSs (with protein)               :: 601
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4870
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_283752"
                     /isolate="SMAG_U16294"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..184)
                     /locus_tag="VLB02_00255"
     CDS             complement(<1..184)
                     /locus_tag="VLB02_00255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56512.1"
                     /translation="MGKFRNFGNEQQRLNIEFKELYIEIKNFLITKGFDTSSEELINY
                     LKKEFYIAIGNGFHQHE"
     gene            complement(333..587)
                     /locus_tag="VLB02_00260"
     CDS             complement(333..587)
                     /locus_tag="VLB02_00260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56513.1"
                     /translation="MPKVLAILNGPEQAELDSMVEHDDRSRHTSFLTEEGWHTVYCRS
                     IEINNAMYEKGIDIVISNDNYKVEYDSAKHTGTLEILEKN"
     gene            complement(852..3005)
                     /locus_tag="VLB02_00265"
     CDS             complement(852..3005)
                     /locus_tag="VLB02_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013781357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HSE56514.1"
                     /translation="MAEGIFKLSNHFKPAGDQPKAIEALLAGLKKGMQKQTLLGATGT
                     GKTFTMANIIAAEQKPTLVIAHNKTLAAQLASEFKEFFPENAVHYFVSYYDYYQPEAY
                     LPVTDTFIEKDAQINKEIDRLRHASTQSLLTRRDVIIVASVSCIYGLGSPVEYEKTNL
                     RLSRGQNISRTELMERLVSIHFTRTNADLEPGSFRAVGNRVEVMPISDTFVYSIELGT
                     GGGFSQSSGRSAQPDIQKSSSGSSIVSMLKIDPVSSRILSEESDIFIFPAKHFITNQS
                     ERERAVKTIEAELKVQLAQFEAEGKLLEAERLRRRTNYDLAMIREVGFCSGIENYSRH
                     LSGKAAGEPPETLFSYFPSTRRSLGKGGQEIKVPDFLLFIDESHVTIPQLQGMYAGDA
                     SRKGTLVEYGFRLPSAKDNRPLKFNEFEERIGQVIFTSATPAKYEREHSEQVVEQIIR
                     PTGLVDPLVEVRPILESGEYHGQVQDFIHEAETEIKKGGRVLATTLTKRMAEDLSTYL
                     KERKIKAEYLHSDIKTIERIQIITKFRKGEFDVLVGVNLLREGLDMPEVSLIGILDAD
                     KEGFLRSEVSLIQTIGRAARNVNGRVILYADTITGSMDRAIGETNRRRETQLAYNKKH
                     GITPQTIQKAIKDITADMESDHSRAVSEELAFDLGVFSEGGKKPTKRAMKGTMSKLIK
                     LKEQQMKQAVKMLEFETAALLRDEIKILREKIDAK"
     gene            complement(3048..>4870)
                     /locus_tag="VLB02_00270"
     CDS             complement(3048..>4870)
                     /locus_tag="VLB02_00270"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HSE56515.1"
                     /translation="SSATTSAASGNNTITFSFLSDGTYSNCTITVTDPTGNASNVLSV
                     SSFTVSAAPILAEVIPIPPTTSDTTPNYTFSSTEAGTISYGGSCSSATTSAASGNNTI
                     TFSTLADGVYSNCTITVTDGNSMASDPLAVDSFTVDTIPDTTSILYGIDGSGQNPDPP
                     HLYILNTTTGQKQTDVGAVGFYVTGMAFHPDTNVLYGSTGNTGSNTKSLITINKDTGA
                     GTLLGALKDGSNGTHTLADIEFRSNGTLYGWSTNGNDLYTVDLTSCNGTSDTNCLVTK
                     VGEFGSSAFGNGLVFDTNDNLYLFRQGDDNFNTLDPDTGLSIEQTNFSNPSGNGYTIP
                     AATINGDGVIYAPRLNYGSSPSDLITIAMDTGTITSLGDTADMLYMDALAFYFPPDTT
                     APAISAISVTATQSTATITWTTDESSSSSVQYGLTTSYGSSGTGSGSTTSHSVTLTSL
                     AACTTYNYKAVSVDSASNSGESSNQTVTTSGCTSSVSGSVARSAVSPVVPFIPTTPTS
                     TPTPSPAAPVVFTRALVLGKKDPAVKKLQQYLNAHSFTISRVGAGSPGKETDFFGIKT
                     KAALIRFQEAHAADILIPLGLKKGTGNLGPRTRAYIATHP"
BASE COUNT         1312 a         1105 c         1041 g         1412 t
ORIGIN      
        1 tttcatgctg atggaaccca tttccgattg caatataaaa ttcttttttt agatagttta
       61 tgagttcctc tgacgaggta tcaaatcctt ttgttattaa aaaatttttt atttctatat
      121 acaattcttt aaattcaatg tttagccgtt gctgctcatt accaaagttt ctaaattttc
      181 ccatgggtat aaatttttac atttttctca atctactcaa ccaatgggca aagtcaagta
      241 attgtattat tttaggagta gttcagacgg ttgtgaaagt agacaaaaga aaaaaccact
      301 ttacagtggt ttatggataa tcaaaatatt ctttaattct tctctagtat ttccaatgtt
      361 ccagtatgct tggctgaatc gtattcaacc ttgtaattat catttgaaat tacaatatca
      421 atgccttttt catacattgc attattaatt tcaatacttc tgcaatatac cgtatgccaa
      481 ccttcttctg ttaagaatga ggtgtggcgt gagcggtcat catgctcaac catagaatca
      541 agttctgctt gttcgggacc attgagaata gctaatactt taggcatata tttttggttt
      601 taatgattta ccacaaaccc cataaggatt tctggaaaac catcaaaacc ttctcaagga
      661 acttaattgt cttacagtag catgtatttt acatattgtc aaatactttc aggctgacat
      721 tcacacaaca aagagtagct tgataaccaa atagggacta gttccctttt taacctcttc
      781 accataacca acactaacca cacctgcctt ttcatagtaa aaggtagccc agattctctt
      841 ggtagcgctt tttattttgc gtcgattttc tcgcgtaaaa tttttatctc atcgcgcagc
      901 agtgctgcgg tttcaaactc gagcattttt actgcctgct tcatttgctg ctccttcagt
      961 ttaatgagct tgctcatcgt tcccttcatc gcccgtttgg ttggcttctt gcctccctcg
     1021 ctaaatactc ctaagtcaaa tgcgagctct tctgaaactg cccggctatg atcgctctcc
     1081 atatcagcag taatatcttt aatcgctttt tgaattgttt gcggcgtaat gccatgcttt
     1141 ttgttgtacg cgagctgtgt ttcgcggcgg cggttcgtct caccaatggc gcgatccatc
     1201 gaaccagtta tcgtatcggc atacagaatt acccgaccgt ttacattgcg ggcagcgcga
     1261 ccgatggttt gaatgagtga cacttcgctc cgcaaaaaac cttctttgtc ggcatctaaa
     1321 attccaatca gcgatacttc aggcatatcg agcccttcac ggaggaggtt caccccaacg
     1381 agtacatcaa attctccttt acgaaattta gtaataattt gaatacgctc gatggtttta
     1441 atatcactat gcaggtactc ggcttttatc tttcgctctt tcaagtaggt tgaaagatcc
     1501 tcagccatgc gtttagtgag ggtcgttgcg agcacccgcc cgcctttttt aatttcggtt
     1561 tctgcttcat gaatgaagtc ttgcacctgt ccgtgatact cacctgattc aagaattggt
     1621 cgcacttcaa ccagcggatc aacaagcccc gttggtcgga taatttgctc aaccacttgc
     1681 tctgagtgct cacgctcata ctttgccggc gtcgcagaag taaaaattac ttgaccaatc
     1741 cgctcctcaa attcattaaa tttaagcggg cgattatctt ttgctgaagg caggcgaaag
     1801 ccgtactcaa caagcgttcc tttccgggat gcatcgcccg cgtacatacc ctgcagttgc
     1861 ggaatggtta cgtgcgattc atcaataaag agtaagaaat ccgggacttt aatttcttga
     1921 ccgcctttgc caaggcttcg gcgcgtcgag ggaaagtatg agaacaatgt ttcaggcggc
     1981 tcgccagcag ctttgcccga aagatgccgc gagtaatttt caatacccga gcaaaaacca
     2041 acttcgcgaa tcatcgccag gtcgtagttc gtgcggcggc gtaggcgctc tgcttcgagc
     2101 agcttccctt cagcttcaaa ctgtgcaagc tgcactttta attctgcctc gattgttttt
     2161 actgcccgct cacgctcgct ttggttggta ataaagtgct ttgccggaaa aataaaaata
     2221 tcgctctcct cagaaagaat ccgtgaactg actgggtcga tttttaacat ggacacaata
     2281 ctagaaccgg aggaggattt ttgaatgtct ggttgagcgg agcgacccga agactgagaa
     2341 aatcctcctc cggttccaag ctcgatactg tatacaaacg tatcagatat cggcatcacc
     2401 tctacgcggt tgccgaccgc acgaaaactg cccggctcta aatcagcatt cgtacgagta
     2461 aaatgaatcg acactaatcg ttccatcagt tctgtgcgag aaatgttctg ccccctcgag
     2521 agcctgagat ttgttttttc atattcaacc gggctcccca aaccgtaaat acaagaaacc
     2581 gaggcaacaa taattacatc acgccgggtg aggagcgatt gggttgaagc gtggcgcagg
     2641 cggtcgattt ctttgttgat ctgggcgtct ttttcaataa aggtgtcggt taccggcaga
     2701 tacgcttcag gctggtagta gtcgtagtac gagacaaagt agtgtacggc gttctcggga
     2761 aaaaattcct taaactcact cgcaagctgc gccgcgagtg ttttattgtg agcaatcacg
     2821 agtgttggct tttgctcagc ggcaataata tttgccatcg taaacgtttt tccggttccg
     2881 gtcgccccca agagggtctg cttctgcatc cccttcttca gccccgccaa aagcgcctca
     2941 attgccttcg gctggtcacc ggctggttta aagtggtttg atagtttgaa tataccttct
     3001 gccataaaca aagggtagat taccacattc gaaaaaaatt aattacttca cggatgtgtg
     3061 gcaatatatg cacgcgttct cggaccaaga ttaccagtac ctttcttcaa tccaagtgga
     3121 atcaaaatat ccgcagcatg agcttcttgg aatctaataa gcgctgcttt tgttttaata
     3181 ccaaagaagt cggtttcttt cccaggtgaa ccagcaccta ctcttgaaat agtaaaacta
     3241 tgagcattta aatactgctg aagtttttta actgctggat cttttttgcc cagcaccaag
     3301 gcgcgcgtaa acacaacagg agctgccggt gaaggagtgg gggtagaagt tggggtagta
     3361 ggaataaatg gcactactgg tgacaccgca gaacgagcaa cggaaccgct gaccgaggag
     3421 gtgcatcccg aagtagtaac tgtttgatta ctactttctc ctgaatttga agcactatca
     3481 accgaaacag ctttgtaatt atacgtcgta caagcagcaa ggctagtgag tgttaccgag
     3541 tgactcgtag tacttcctga tcccgttcca gaactgccgt acgaggtcgt aagcccatac
     3601 tgcaccgatg aagatgaact ttcatccgtt gtccatgtaa tggtagctgt gctttgggta
     3661 gcggttacag aaatggcaga gatagctggc gcagtagtgt caggaggaaa gtaaaatgcg
     3721 agtgcatcca tatacaacat atcggcagta tccccaagag aagttatcgt acctgtatcc
     3781 atggcgatag tgatcaagtc agacggcgat gaaccatagt ttaagcgcgg agcatatatc
     3841 acaccatctc cattaatcgt agcagcggga atagtatatc cattccccga tggattagaa
     3901 aaatttgttt gttcaataga aagcccggtg tctggatcaa gcgtgttaaa attgtcatcc
     3961 ccttgtctaa acaggtacaa attatcatta gtatcaaata caagaccatt cccaaatgca
     4021 cttgagccaa attcaccaac tttagtaaca agacagtttg tatcactggt tccattgcac
     4081 gatgttaggt caacagtata gagatcattc ccgttggttg accacccata gagtgttcca
     4141 ttggaacgaa attcaatatc agccaaggtg tgagtgccat tactcccatc ttttaaagca
     4201 ccaagcagtg ttccagcacc agtatcttta ttgatagtta taaggctctt tgtattcgac
     4261 ccagtattcc cggtagatcc gtagagtacg ttggtatcag gatgaaatgc catgccggta
     4321 acataaaatc ctaccgctcc aacatcagtc tgtttttgcc ctgtggtggt atttaaaata
     4381 tacaagtgcg gcggatcagg attttgtcct gaaccatcaa taccatagag aatagaagta
     4441 gtatcaggaa tcgtatcgac agtaaatgag tcaacggcaa gtggatcact cgccatactg
     4501 tttccatcag tcactgtaat cgtacaattg gagtagacac cgtccgcaag cgtgctaaat
     4561 gtaatagtgt tgttccctga agctgccgat gtcgtagcac tcgagcatga gccgccgtaa
     4621 ctgatggtac ccgcttcggt ggaactgaag gtgtagttgg gagtggtatc ggaagtggta
     4681 ggagggatag gtatcacctc agcgagtatt ggagcagcac tcaccgtaaa actagacact
     4741 gaaagcacgt tgcttgcatt accagtagga tcggtcactg taatagtgca attagaatat
     4801 gttccatctg aaagaaaact aaatgtaata gtgttgttcc ctgaagctgc cgatgtcgta
     4861 gcactcgagc
//
LOCUS       DATIBQ010000011         6428 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U16294
            k141_1281979, whole genome shotgun sequence.
ACCESSION   DATIBQ010000011 DATIBQ010000000
VERSION     DATIBQ010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935789
            Sequence Read Archive: SRR25158331
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 6428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:58:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 637
            CDSs (total)                      :: 603
            Genes (coding)                    :: 601
            CDSs (with protein)               :: 601
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6428
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1281979"
                     /isolate="SMAG_U16294"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..119)
                     /locus_tag="VLB02_00275"
     CDS             complement(<1..119)
                     /locus_tag="VLB02_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018278859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HSE56516.1"
                     /translation="MAKIKLIPLGDRVIVSLAAEKKTLSGIIIPDTASKERPE"
     gene            261..482
                     /gene="secG"
                     /locus_tag="VLB02_00280"
     CDS             261..482
                     /gene="secG"
                     /locus_tag="VLB02_00280"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HSE56517.1"
                     /translation="MSHFIPYLQLGAALLLIVLVVLQRSSGDIGGAFGGDGAEFFRTR
                     RGFEKFLFIATIVIAVIFVATSAGVLLLK"
     gene            494..2221
                     /locus_tag="VLB02_00285"
     CDS             494..2221
                     /locus_tag="VLB02_00285"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HSE56518.1"
                     /translation="MKNFFRFLRAWSFPSRKRLVQAFTATHGATRVFFSALLVCTVII
                     GAALILRINEYISIRVPVAGGSFTEGVVGAPRFINPILAVSDTDRDLTTLVYSGLMKR
                     LPSGEIVPDSAEGYTVSSDGLTYTFTLKQNLTFHDKTPLTADDVVYTIAQVQNPAVGS
                     PRRTSWAGITIQKIDDRTVAFTLPKPFGPFLEATTIGILPAYLWKETTPETFSFLALN
                     TEPIGSGPYKFSSFEKKRNGIPTSYTFTRFNRYALGAPYIKKIFIQMYGNQDELVRAI
                     NNGDVVSAAALSPEAVAHITNKHIHIHTAVLPRVFGVFLNPNQKSLFKDTALVALIDE
                     ALDKEALVTGVFGTYARRIDGPVPFQPVIDRAVPPKRSHEEIQAAFEAKGWIKNSSGI
                     LEQKGSRTPLYFSLVTADTPELVKVAAAIRDTLSTYGIAVELKVYDLGALSQNAIQSR
                     NFDALLFGHVIENEADLYSFWHSSQRSYPGLNITQYANSRVDDLLEALLKTTDQETVD
                     KNLALLDATIRTDHPALFIYLPHFLYATTDAIGGISLDRISQPSDRFLGVQSWYRETE
                     PVWKLFNKQ"
     gene            2234..4123
                     /locus_tag="VLB02_00290"
     CDS             2234..4123
                     /locus_tag="VLB02_00290"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HSE56519.1"
                     /translation="MEPQQTPQTHRASTAPHTSPQKRSVHRTGRHSSGGQFRGRSNFR
                     PRTPSPNEEGFRTNTEKKMVYVPDPAPGVIRIIPLGGVEEIGKNMTAIEIGNDIIVID
                     AGMYFSNETTPGIDYVIPNTRYLEERKDKIRALVITHGHLDHIGGVPLVLSRIGNPPV
                     YSRNLSVLLMRKRQSEFPHLPALNAITVEKDEVVTLGNIRVKFWGVTHTIPDSMGIII
                     ETPYGNIVTPGDYKLDQVDGVVSESEEKEYKQFDTANNLLLMTDSTNVENEGFALAEI
                     KVHQGLENILRRIQGRVIIAAFASHITRLIKIIQIAEEMGKKVILEGRSMKTNIEVAI
                     QAGLIQPQKGTLISIDEIDRHPPNRIIVLSTGGQGEPFSALNRAANKTNKKFSLKKGD
                     TIILSASIVPGNERQVQAMKDGLSRQGVKIISFRTPGEDFVHATGHGNKEDIKWLHRK
                     MHAKFFIPIHGNHYHLELHKELAMELGMPEDHIVVPDNGSIIEIVEEGKKIVVRKEKA
                     PASPMMVDGFSIGDEQNVVIRDRQMLAEDGMFVVIAVIDAKTGQLKKSPDIISRGFVY
                     LKESQDLIRHSRTLISRTIEQSCRNQNPINFDYVKNTVTDTVAKYLYQKTAKRPLVIP
                     VVLSI"
     gene            4137..5285
                     /locus_tag="VLB02_00295"
     CDS             4137..5285
                     /locus_tag="VLB02_00295"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSE56520.1"
                     /translation="MTRHLRILFASSFFFSAHIAILAYTNSSSLSRFASDSTISFIYA
                     GSSALSLLWLAIAPTLLRRYGNWKLAIGTFGVAIVTLLFLATAAAPVFFAALFAFYFS
                     LNILITYVLDIFIEHYSTNSGTGNIRGIYLTSVNLAWVGAPIIAGLLIFNYGFSAIYG
                     VAALLLIPAALLIASSEHGFRDQIYKPARFFRSLSVLKTNKSVRLIIFLNFLLQFFYA
                     WMVIYAPLYLTKVHGFSWETIGILFTFMLLPFIIFQYPAGKIADKILGEKELLVAGFL
                     IMCLATIFFALLPGGSVLAFGIALFTTRVGASIVEVMCDTYFFKQITDKETTFISFYR
                     ETIPLAYIIAPLWGVLVLASGSYNLLFFILGALMLVGAGFSLTLKDTR"
     gene            complement(5291..>6428)
                     /locus_tag="VLB02_00300"
     CDS             complement(5291..>6428)
                     /locus_tag="VLB02_00300"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HSE56521.1"
                     /translation="IVAAVARLVPVSEQVAGQESSTGQLAQAPAAPKPLSVTDVVRVA
                     NPAVVSIEATKTVPTYETQYSTQNPFGSMFPGFSFQLPSRKQTGTEEKVVSGGTGFFV
                     SKDGYLVTNRHVVEDTDAKYTITTTGGKKYTATVIARDQVLDVAILRVTGSTFSYLNL
                     GDSDTLELGQSVIAIGFALGEFQNSISTGVISGLSRSIVAGGGAGGTEQLDKVIQTDA
                     AINPGNSGGPLLDMTGKVIGVNVAVAQGSQNVGFSLPINSIKPVITSVRETGKIVRPY
                     LGIRYVPVTKELAKANNLSVEYGVLVQRGQSATDLAVIPGSPANKAGIVEGDIILEID
                     GQKLDEKSSFANVIRTKAVGQVITLHMLSRGQTKTLSVKLEAAP"
BASE COUNT         1692 a         1695 c         1369 g         1672 t
ORIGIN      
        1 tgctcggggc gctcttttga agcagtgtca ggaataataa tacccgaaag agtctttttt
       61 tcagccgcca gagaaacaat gacgcgatct ccgagcggaa taagttttat ttttgccata
      121 cctgtttaat aaccaaataa caaaatccaa taatcaaaat aaatctgtaa tcagcatgca
      181 gccctgctgc gcgtaagtat tatagccaaa tcgttgctcg atacaagcca gtatgctata
      241 gttaaagacg ctagtttatt atgagtcact ttatccccta cttgcagctc ggtgctgcgc
      301 tcctccttat cgtcctggta gtgctccaac gttcaagtgg agatattggc ggagctttcg
      361 gcggtgatgg tgcggagttc ttccgtactc gtcgcggatt cgaaaaattc ctctttattg
      421 caacaatcgt gattgcagtt atttttgttg caaccagtgc cggggtactc ttgctcaaat
      481 aaataggtta cctatgaaaa acttcttccg ctttctgcgc gcatggtcgt ttccatcacg
      541 gaagcgtctc gtacaagctt ttaccgccac gcacggcgct actcgagtgt tctttagtgc
      601 ccttttggta tgcacggtta ttattggtgc cgcacttatt ctccgtatca acgaatatat
      661 ttctattcgt gttcctgtcg ccggcggcag ttttactgaa ggtgttgttg gtgctccccg
      721 atttattaac cccatactcg cagtgagtga taccgaccgc gaccttacca cactcgtata
      781 ctcaggactc atgaagcgcc tgccgagcgg cgagattgtc cccgatagcg ctgaaggcta
      841 tactgtaagc agtgatgggc tcacctacac gttcaccctc aagcaaaatc tcaccttcca
      901 cgacaaaaca ccgctcactg cagatgacgt tgtctacacc attgctcaag tgcaaaaccc
      961 tgctgttggg agcccgcgcc gcaccagctg ggctggcata acaatccaaa aaattgacga
     1021 ccgcaccgtt gcctttaccc tgccaaaacc atttggtccg tttttagaag caacaactat
     1081 cggtatttta cctgcatatc tctggaaaga aacaacccct gaaacattca gcttcctggc
     1141 actgaatact gaacctattg gctctggtcc atataaattt tcaagtttcg agaaaaaacg
     1201 aaacggtatt cctacctcgt acacctttac tcgtttcaat cgctacgcac tcggtgcacc
     1261 ctacataaaa aagattttta tacaaatgta cggcaaccaa gatgaactcg tccgggcgat
     1321 taacaacgga gatgtagtca gcgctgctgc tctctctcct gaagcagtcg cacacataac
     1381 aaataaacat atccacatac acactgcagt gctccctcgc gtgttcggtg tgtttttaaa
     1441 tccaaatcag aagagtttat ttaaagatac tgcgcttgtt gcccttatcg atgaggcgct
     1501 cgataaagaa gcccttgtta ccggagtatt tggcacatac gcccgccgga ttgatggtcc
     1561 tgtccccttt cagccggtaa tcgatagagc ggtaccccca aaacgatcac acgaagaaat
     1621 tcaggcagca tttgaagcaa aggggtggat aaaaaattcg agcgggatcc tcgagcaaaa
     1681 aggtagccgc acaccgctct acttttcact cgttactgcc gacactcctg aactagtgaa
     1741 ggttgcagct gcaatccgcg atacacttag tacctacggt attgctgttg aactaaaagt
     1801 gtacgacctc ggcgcgctct ctcagaatgc aattcaatct cgaaattttg acgccctcct
     1861 ctttgggcat gtgattgaaa acgaagccga cctctacagt ttttggcact catcgcagcg
     1921 cagctacccc ggactcaaca ttacccagta cgccaactcg cgcgttgatg acctccttga
     1981 agcactttta aaaacaaccg accaagaaac cgtagataaa aatcttgcat tacttgatgc
     2041 aactattcgc accgaccacc cggcactctt tatttacctg ccgcacttcc tctatgcgac
     2101 caccgatgcg ataggcggaa tcagtcttga ccgtatcagc cagcccagtg atcgattcct
     2161 cggcgtacag tcatggtaca gagaaacaga gcccgtatgg aaattattta ataaacagta
     2221 accacaaatt agtatggaac cacaacagac accacaaaca catagggcat caactgctcc
     2281 acacaccagt ccacagaaac gaagtgttca tcgcaccggg cgccacagta gcggcgggca
     2341 atttcgcggt cgcagtaatt ttagacctcg taccccaagc ccaaacgaag aaggctttag
     2401 aaccaacacc gaaaaaaaga tggtatacgt tcctgaccct gcccctggcg ttatccgcat
     2461 aattcccctc ggcggcgttg aagaaattgg caagaacatg accgctatcg aaatcggcaa
     2521 cgacattatc gtaattgatg ccggcatgta cttctcgaac gaaaccactc caggtatcga
     2581 ctacgtgatt ccgaacaccc gctaccttga agagcgaaaa gataaaattc gcgcgctggt
     2641 gattacccac ggacacctcg accacattgg tggtgtgcca ctagtgctct cgcgcatcgg
     2701 caatccgcct gtttactcgc gcaatttaag tgtgctcttg atgcgcaaac gtcagagtga
     2761 gtttccacac ctacctgcac taaatgctat tacggttgaa aaagacgagg tagttactct
     2821 agggaacatc cgagtaaaat tttggggtgt tacgcacacc attcctgatt cgatggggat
     2881 tattattgaa actccctacg gaaacattgt taccccgggc gactacaagc tcgaccaagt
     2941 agatggtgtc gtgagtgaat cagaagagaa agagtacaaa cagtttgata ctgccaacaa
     3001 cttgctcttg atgaccgact caaccaacgt tgaaaacgaa ggttttgccc tagccgaaat
     3061 aaaagtacac cagggacttg aaaatatttt gcgccgcatc caagggcgcg tcattattgc
     3121 tgcgttcgcc tcgcatatca cccggctcat caaaattatc cagattgctg aagagatggg
     3181 caaaaaagtg atccttgaag gacgctcgat gaaaacaaat attgaggttg ctattcaagc
     3241 cgggttgata cagccgcaaa aaggcacact catttcaatt gacgaaatcg accgtcaccc
     3301 gccaaaccgg atcattgtgc tctcaaccgg cggacaaggc gaacctttct ctgcactcaa
     3361 ccgcgctgca aacaaaacaa ataaaaaatt ctcactcaaa aaaggagaca caatcattct
     3421 ctcggcttcg attgtgcccg gtaatgaacg ccaggtgcag gcaatgaaag atggtctctc
     3481 acgccaggga gtaaaaatta tcagcttccg cactccaggc gaagactttg tgcacgctac
     3541 cggacacggc aataaagaag atatcaagtg gctgcaccga aaaatgcacg caaaattctt
     3601 tattccaatt cacggcaacc actaccacct ggagctccac aaagagcttg cgatggagct
     3661 tggcatgcct gaggatcaca ttgttgtccc tgataacgga tcaattatcg aaattgtcga
     3721 agaaggaaag aaaatagtag tacgaaaaga aaaagcaccg gcgagcccga tgatggttga
     3781 tggcttctcg attggtgacg aacagaatgt agtgattcgc gaccgccaaa tgcttgctga
     3841 agacggcatg tttgtggtga tcgccgttat tgacgcaaaa acagggcaac ttaaaaaatc
     3901 gcccgatatt atttcacgcg gcttcgtata tcttaaagaa tcacaagatt taatccggca
     3961 ctcccgcacg ctcattagcc gcaccatcga gcaatcgtgc agaaaccaga acccgattaa
     4021 ctttgattat gtaaaaaata ctgttaccga cacggttgca aaatacctct atcaaaaaac
     4081 agcaaaacgc ccgctcgtta ttccagtagt gctctctatt tagtacaata cgggtaatga
     4141 cacgccacct gcgcattttg tttgcaagct ccttcttttt ctcggcacat attgctatct
     4201 tagcgtatac caattcaagc tcactctctc gctttgcgag tgacagcact atcagtttca
     4261 tctatgcggg cagctcggca ctctcacttc tatggcttgc aattgcacca acactcctgc
     4321 gccgatacgg caactggaag ctggcaatcg gcacctttgg cgtggcaatt gttacattgc
     4381 tttttttggc aaccgcggca gcaccggtat tttttgccgc actttttgca ttctattttt
     4441 cgctcaacat tctcattacc tacgtgctcg atatttttat cgaacactac agtactaata
     4501 gtggcactgg taacatccgt ggtatttacc ttacctcagt caatcttgct tgggtcggag
     4561 caccgattat tgcaggatta ctgattttta attatgggtt cagtgcaatt tacggcgtgg
     4621 cagcactact tttaattcca gcagcgctcc tcattgcttc aagcgagcac ggctttcgcg
     4681 accaaatcta taaacctgcg cgctttttta gaagcctgag cgtgcttaaa accaataagt
     4741 cggtacgact tataatattt ctcaatttcc tgctgcaatt tttttacgcc tggatggtta
     4801 tttacgcgcc gctctatctc actaaagtac acggattttc gtgggaaacc atcggtattc
     4861 ttttcacttt catgctgctg ccttttatta tttttcagta cccggcagga aaaattgccg
     4921 ataaaatcct aggggagaag gagctcctcg tcgcagggtt ccttattatg tgtctcgcta
     4981 ctattttttt cgcgctcctt ccgggcggca gcgtgctcgc ctttggcatc gccctattta
     5041 ctactcgtgt cggtgcgagc attgtcgagg tgatgtgcga cacctacttt tttaaacaga
     5101 ttaccgataa agaaacaacc ttcatcagct tctaccgcga gaccatcccg cttgcctaca
     5161 tcatcgctcc gctctggggc gttcttgtcc tcgccagcgg ctcctacaac cttctcttct
     5221 ttatcctcgg cgcactgatg ctcgtcggcg caggattcag ccttacccta aaagatacgc
     5281 ggtagttttt ctacggtgcg gcttcgagtt ttacagagag cgttttggtt tgtccgcgag
     5341 agagcatatg tagggtaatt acttgcccta ctgctttggt gcgaatgacg ttcgcaaagc
     5401 tcgatttttc atcaagtttc tgtccatcaa tttctaagat aatatctccc tctacaatac
     5461 ccgccttatt tgccggagag cctggaataa ccgccaagtc agtagcactc tgcccacgct
     5521 gcaccagcac cccatattcg acagaaagat tatttgcttt ggcaagctct ttagtaacag
     5581 gcacgtagcg aataccaagg tacgggcgga caattttacc cgtttcgcgc actgaggtaa
     5641 tcaccggttt aatcgaattt atcgggagcg aaaaaccaac gttttgactt ccctgtgcta
     5701 ctgccacatt tactccaatt acttttccgg tcatatcaag cagtggtccg cccgagttcc
     5761 ccgggttaat tgctgcatca gtttgaatca ccttgtcgag ctgctctgtg ccacccgcac
     5821 ctccacccgc aacaatcgag cgtgagagtc ctgaaatcac cccggttgaa atcgaattct
     5881 gaaactcacc aagcgcaaaa ccgattgcga tcacactttg cccgagttcc agcgtgtcag
     5941 aatctcccaa attcaagtac gaaaacgtac tccctgttac ccgaagaata gcgacatcga
     6001 gcacctgatc acgagcaata acggtagcgg tgtacttttt tccgccggta gtggtaatag
     6061 tgtattttgc atcggtatct tcaaccacgt gccgattggt taccaagtag ccatcttttg
     6121 aaacaaagaa tccagtaccg ccgctcacca ccttctcctc agttccagtc tgttttcgac
     6181 tcgggagctg gaatgaaaac cctgggaaca tgctgccgaa tgggttttga gtgctgtact
     6241 gtgtttcgta cgttggtact gttttagttg cctcgattga aacgacagca ggatttgcaa
     6301 cacgcaccac atcggttacc gagagcggtt ttggtgctgc gggcgcctgc gctaattgcc
     6361 cggtactact ctcttgccca gcaacctgct ctgacactgg aactaaccgc gctactgcgg
     6421 caacaatc
//
LOCUS       DATIBQ010000012         8198 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U16294
            k141_1944681, whole genome shotgun sequence.
ACCESSION   DATIBQ010000012 DATIBQ010000000
VERSION     DATIBQ010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935789
            Sequence Read Archive: SRR25158331
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 8198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:58:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 637
            CDSs (total)                      :: 603
            Genes (coding)                    :: 601
            CDSs (with protein)               :: 601
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8198
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1944681"
                     /isolate="SMAG_U16294"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..968)
                     /locus_tag="VLB02_00305"
     CDS             complement(<1..968)
                     /locus_tag="VLB02_00305"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HSE56522.1"
                     /translation="MFENNSPWIKQLNRTRPIEPLDQNIDTEVVIIGGGIAGVTSAYF
                     ILTQTNKKVLLIEANKIAHGATGHNAGQITSYFEKPFTEIVAAYGLHRAAQAQRAIEE
                     DARGLLEHIYSHAQLSTPRSEFMGYEGLATLAHVLEILQELAVKAEAGLRIRHLLVAR
                     EWDEHRLIPAQFAGLYSLVSQKNIFSLLETENNIYIAAAPFLSGCMNSALFSEELVGF
                     LLITYKDRFMLREHTPVSAIALERDEVVLSAGTNTISCEQVLLCTNGFESVQITNNAG
                     GDIDGKFHMTVEGVVGYMGAYKESLTKQPFAACYANPLYTTNREYY"
     gene            complement(1017..1295)
                     /gene="rnpB"
                     /locus_tag="VLB02_00310"
     ncRNA           complement(1017..1295)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VLB02_00310"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            1357..1815
                     /locus_tag="VLB02_00315"
     CDS             1357..1815
                     /locus_tag="VLB02_00315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56523.1"
                     /translation="MEHKTTVILFDEYQSKLFDSNSHNEYRFRIVQKELILPILLIQE
                     VPLRQRLIRLADVLEIDYLMVDLGFEYGLSTAESIYFVLTLITIAKEEGIKVIPYIKF
                     GTLSSSTLSFAKILIKEITKRDLLIFEIPIENLNPFVKAIEDVSYEDIGE"
     gene            complement(1874..3598)
                     /locus_tag="VLB02_00320"
                     /pseudo
     CDS             complement(1874..3598)
                     /locus_tag="VLB02_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014782020.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
     gene            complement(3617..4888)
                     /locus_tag="VLB02_00325"
     CDS             complement(3617..4888)
                     /locus_tag="VLB02_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015466287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HSE56524.1"
                     /translation="MIALMVRKRSGSVVDFDQARIERAIAKAYAATSTGLPDAELSEL
                     VERILTEIEKTFGEQLIPSVEEIQNIVEKAIATKGDFDVAKAYILYREERNKERKRDI
                     FKKRVYLKPYEYPELYEYVSAIRHSYWIHTEFNFVGDIQNFKVNVTEAERNVIKNAML
                     AIAQIEVAVKTFWGDIYKKLPKPEIGAVGATFAESEVRHTDAYAHLLEILGLNEAFKS
                     IGDVPALMKRVHYLEKSLANARSEENREYSQAILLFSLFIEHVSLFSQFLVIMSFNKH
                     KNLFKGISNAVEATSKEEQIHGLFGIELINIIKEEHPEWFDAEFEEGVVKICKEAFES
                     ETQVVEWIFEKGELEFMSKAVVIEFIKQRFNSSLQSIGIRTIFPTDEKLLKESDWFYD
                     EVIATKHGDFFNKKSINYNKKSKSITSDDLF"
     gene            complement(5031..5528)
                     /locus_tag="VLB02_00330"
     CDS             complement(5031..5528)
                     /locus_tag="VLB02_00330"
                     /inference="COORDINATES: protein motif:HMM:NF014678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HSE56525.1"
                     /translation="MNNNAKKAVIIGITILIATAFFAVWKSYRMPASTEAVTPQAMSE
                     QSTGPRVTIGDTTITVTVADTEAVRQQGLSGKESLAQDEGMLFFFDTPGKYGFWMKDM
                     HFPIDIIWIDAGWQIVDVTHTLTPETYPKVFFPQASVQYVLEVPAGFSTQHKLLIGQL
                     VQFEK"
     gene            complement(5709..7355)
                     /locus_tag="VLB02_00335"
     rRNA            complement(5709..7355)
                     /locus_tag="VLB02_00335"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            complement(7616..>8198)
                     /locus_tag="VLB02_00340"
     CDS             complement(7616..>8198)
                     /locus_tag="VLB02_00340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56526.1"
                     /translation="LAVEPEFFVTKVGAKRYRLYVVFNNIPEDSVLFGPEKTPENGCI
                     VFGKRMIFSFAHENNGVKLMRDPASRGKNDLRIRRASVMTSEQHVTKKRVNGKKVSDT
                     TEIPVQIKNTYRSEEFSGSPRFFQVFKVVTVETRKENGTIEYFTPTRITGSVRYHVNS
                     QNPKKMDGEFEQVQVRTAHFDIELPKSRKKVHK"
BASE COUNT         2117 a         1990 c         1670 g         2421 t
ORIGIN      
        1 taatagtact cgcggtttgt tgtatacaac gggtttgcat aacacgccgc aaagggttgc
       61 ttggtgagcg actctttgta cgctcccatg tatcctacga ctccttcaac agtcatatga
      121 aatttaccat caatatcccc gcctgcgtta ttcgtaatct gcaccgattc aaatccattt
      181 gtacacaata atacctgctc gcacgaaata gtattcgttc ctgcactcaa aacaacttca
      241 tcacgctcga gcgcaatagc agataccggt gtgtgttctc ggagcataaa acggtcttta
      301 taggtaataa gcaaaaaccc aacaagttct tcagaaaaga gtgcgctgtt catgcaacct
      361 gagagaaatg gtgcagcagc aatgtatata ttgttttctg tttctaataa agaaaaaatg
      421 tttttctgac ttactaaact atagagtcct gcaaactgtg cagggataag ccggtgctcg
      481 tcccattcac gtgctactaa aaggtgccga atacgcagac cagcttcggc ttttactgca
      541 agttcttgga gtatttcgag cacatgtgca agagttgcca acccttcgta ccccataaac
      601 tcagatcggg gagtacttag ttgtgcatga gaatatatat gctcaagcaa tccccgagcg
      661 tcttcttcaa ttgctcgttg cgcctgcgct gcccgatgca acccataggc agcaactatt
      721 tcggtaaatg gtttttcaaa gtaactagta atttgtccgg cgttgtgccc cgttgctcca
      781 tgagcaattt tatttgcctc aatgaggagt acttttttat tcgtctgcgt gagaataaag
      841 tatgctgatg ttacccccgc aattcctcca ccaataatga cgacttcggt atctatattt
      901 tgatcgagcg gttcgattgg gcgtgttcta ttgagctgtt tgatccacgg agaattattt
      961 tcgaacatag tagtgttatt gtaacagaaa aagtccgccg atttggcgga ctttttcata
     1021 ttattgtact cgtaagccga attctgtttt agtgatcatt tatctgggag taatgttgcc
     1081 actaccctca agcggcactt cgattttcat cgacacgacc ttgcacacag ataggcattt
     1141 agccgtttca gccggactta accgggtcgt ttctgttcgc agccctatgg ttacccacga
     1201 cgggcgttac ccgctacctg tacgaattta atcgttgtgt gttcggactt tcctcaccaa
     1261 tcattactga ctagcgcgat cattcggtac aacaaaaata ctatacaata gttgacaaaa
     1321 aatgcaataa atgtaggatt tataaaaaaa taaacaatgg aacataaaac cactgtgata
     1381 ctctttgatg agtaccaatc aaaattattt gatagcaatt ctcataatga gtatcgcttc
     1441 cgcatagttc aaaaagaact tattctcccc attttactaa tacaagaggt acctcttaga
     1501 cagcgtttaa tacggttagc tgatgttctg gagatagatt atctcatggt agaccttgga
     1561 tttgaatacg gactatcaac agctgaatct atctactttg tgcttacatt aatcaccata
     1621 gcaaaagagg aaggcattaa agttattccg tatataaaat tcggtacttt aagcagttca
     1681 accttatcat ttgctaaaat attaattaaa gaaatcacta aaagagattt gttaattttt
     1741 gaaataccaa ttgaaaatct aaaccctttt gtaaaagcaa ttgaagatgt atcttatgaa
     1801 gacattggcg agtaagcaaa aactgtagcg ttagctacag ttttttattt ttcagttacg
     1861 cctcacagcc aacacagaca ttatcttcaa taatcctcac cttgggcttt tttgcatcct
     1921 gctcttggag cttctttctg ccaaactgct gcgctgcgtt catactgtgc tggtagtaga
     1981 gggttttaat tcccttctcc cacgcataga ggtacagcga gttgagatcc ttcgccgaga
     2041 ttgttgggtc aatcataata ttgagcgact gcgcttgatc aatatacttt tgacgctcgg
     2101 cagcctgctc gataatcact ttttgatcaa tttcgctaaa agtgcggaat actttctttt
     2161 cgtgctcagt gagaaaatcg agatgctgga ctgaaccatc gttgtcgcga atattcttcc
     2221 atacctcagt ggtattcatc cctttttctt caagcaatgt ttcaagcgtt gggttcttaa
     2281 tcgttacttt cattttatct acatctttca caaacgcgtt tgacatgagc ggctcaatac
     2341 cttgagaaac ctgccccaag ataaacgcgg aagatgtggt tggagcaatt gcaaggagtg
     2401 ttgtatttcg acgaccgtac ccctttaata cttctggctc gccgtattct tttgccatat
     2461 ccactgaagc cttgtatgcc ttttcgcgaa taagtttaaa cacttcttcg ttgagagctg
     2521 ccgcttcagg gctttcaaaa gaaatgttgt tctcctgcag gaacgtgtgc cagccgagca
     2581 cacccaaacc cagcgcgcgg tgctcttttg caaacttgtg cgcacgctgc ataaagtaga
     2641 acgcggactg tttgtgagga tcagtactgt cgcggaagct ctcgagccgc tcgacaaact
     2701 ccccaatcac ggtatcgagg aacatagcaa gcgtttcaac agcatcagta tctttccact
     2761 catggtagtg acggatgttg atcgaagaga gacagcagac gaatgagata tcgtccgcag
     2821 tcggaagaca gatctcagaa cagaggttgc tattatgaat tctcataccc ttatctttat
     2881 acacctcaac ggtattatta ttcaccgtgt cactaaagaa aatatacgga tacccgatct
     2941 cggttctgcg ctgaataatc tttgcccata tctttcgctt ctcctgatca ccgccgatca
     3001 tcgccttaag ccatacatct cccacggtaa cagcgtacgt agattcctga atcggatgtc
     3061 cctcctttcc aatattcaaa tactcctcaa tatcatcgtg ctcaaccgga aggtacggtg
     3121 cgcagtgtcc gcggcgaacg gtgccttgac tgacgatatt gataaggtta tcgaagagtt
     3181 ccaaaaagtg caccgacccc gacgagtgcc cgttgtcttt gatgagcgaa ccgcgaggac
     3241 gcaaccctcc taaatatagg gatgtaccgc cgccatactt ggtcatcatg ccaacttcag
     3301 catgagtata gaggatacgc gacatgtcat cgtcaacgaa agacccgaaa cagctgattg
     3361 gaagtccgcg agaatagccg tagtttgccc agataggagt cgcgagcgag tagaagccgc
     3421 gcgacatgta gtcgtaaaac ttgtcggcaa atcccggctt cgcaaggtgc cgctccgcga
     3481 tctcggcgat ctcgcgaatg cgctcctcag cggtcatctc tccgatgaga tacccttttt
     3541 tcaaaaattt tctactgttt tcgtttaacc aatacatgtg ctgtgtctta cgtttacgct
     3601 gttaatgcga tttctgttaa aagagatcat cgctggtgat acttttactt tttttattgt
     3661 aattgatcga ttttttgtta aaaaagtctc catgctttgt cgcaataact tcatcgtaaa
     3721 accagtctga ttctttgagc agcttttcat cggtcggaaa gatggtgcga ataccgatac
     3781 tttgcaacga gctattaaag cgctgcttaa taaactcgat aaccactgct tttgacataa
     3841 actcaagctc tcctttttca aaaatccact ctaccacctg agtctctgac tcaaacgcct
     3901 ctttgcatat cttgactaca ccctcttcaa attctgcatc aaaccattct gggtgctctt
     3961 ctttgatgat attgatcagt tcgattccga ataatccatg aatttgttct tcttttgatg
     4021 ttgcctcaac ggcatttgat atccctttaa acaaattttt gtgcttatta aaggacatga
     4081 taaccaagaa ctgagagaag agcgaaacgt gctcgataaa gagcgagaag aggagaattg
     4141 cttggctata ttcccggttt tcctcgctcc gcgcattcgc cagagacttt tcaaggtagt
     4201 ggacacgctt catgagcgca ggaacatcgc cgatactctt gaatgcttcg ttgagcccga
     4261 gtatttcgag caagtgcgcg taagcgtcgg tatggcggac ctcactttct gcaaaggttg
     4321 cgccgactgc tccgatttct ggcttgggga gctttttata gatatctccc cagaatgttt
     4381 tgacggcaac ttcaatctgg gcaatagcga gcattgcatt tttgataaca tttcgctctg
     4441 cttcggtaac atttacttta aaattctgaa tgtcaccgac aaagttaaat tcagtatgaa
     4501 tccagtacga gtgccgaatg gcgctcacgt actcgtagag ttctgggtat tcatagggct
     4561 taagatagac tctttttttg aaaatatccc gcttgcgctc tttatttcgc tcctctcggt
     4621 ataaaatgta cgcttttgca acatcaaaat ctcccttcgt tgcaattgct ttttcgacaa
     4681 tattttgaat ttcctcaaca gagggaataa gctgctcgcc aaaggttttt tcaatctctg
     4741 taagaatacg ctcaacgagc tccgagagct ctgcatccgg cagaccagtg ctggttgcag
     4801 catatgcctt tgcaatagca cgctcaatac gtgcttgatc aaaatcaacc actgatccgc
     4861 tccgttttcg caccatcaag gcaatcatag atagagcagt atacaaaaaa ttttcgggta
     4921 aaaggtatgg tgcatcattt tttttcggct cgcgaaatga aaaaaataat ttgtaaagga
     4981 gctcgccgag agagtattat gtgagcgtag tttttaccaa tgtttcgaag ttatttttca
     5041 aactgcacta attgcccaat taaaagtttg tgttgtgtgg aaaaaccagc cggtacttca
     5101 agcacatact gtaccgacgc ctgtggaaaa aatacttttg gatatgtttc aggagtgagc
     5161 gtgtgagtaa catcgacaat ctgccaccct gcgtctatcc atataatatc aatcggaaag
     5221 tgcatgtcct tcatccaaaa cccatatttc cccggggtat caaagaaaaa cagcatgcct
     5281 tcatcttgag caagtgattc ttttcctgaa agcccctgct gccgcactgc ttcagtatcg
     5341 gcaactgtta ctgtgattgt agtatctccg atggttaccc gaggaccagt actttgctcg
     5401 ctcatcgcct gcggagttac tgcttcagta ctcgctggca ttctataact tttccagaca
     5461 gcaaaaaaag cagtagcgat tagtatagtg ataccaataa ttactgcttt ttttgcgttg
     5521 ttgttcatat tcacctttct tttgcctcgt tctaccgctt ccgcgctcaa ttgatgcgta
     5581 gaaacggttt tgtgtgcgct cacccttgca cagggagttt ggagaatgcc aaaccaccat
     5641 tttttgtacc tcgacttgcg tcgagagcga ccggatcaat tctgtgaata aacacagaac
     5701 aagtttctct ttgagttatt ccacgaccag cttccgctag ccgtgccttg ttacgactta
     5761 ttccctgtca ccgagcttac cgtaggcccc tcttgtgagg agatttcggg tactcccggc
     5821 tcccttgaat tgacgggcgg tgagtacaag actcgagaac gtattcaccg cagtttggct
     5881 gacctgcgat tactagcgat tccagcttca tgaagtcgag ttgcagactt caatccgaac
     5941 tagggctacc tttgaggatt ggctcaggat cgctccgtcg ctacccactg tagtaaccat
     6001 tgtattatgt gtgtggccca aggcatcaag ggacatgctg acctggcgtc atcctctcct
     6061 tcctcccagc cctttgttcg cgaagcgaac aaaggaataa tcaatatcca agcaccaata
     6121 atcaaacgtt tcgtgtttgg aatttggtat ttgtgttatt ggttattgcc ctttgcgttc
     6181 gtgcgcaaag ggctgggcag tctcgcctga cacgtataac agacgacgag ggttgcgttc
     6241 gttagcccac ttaagggaac aattcaaacc acgaactgac gacggccatg catcacctgt
     6301 ccaaccgtcc ttgcggacga caatagtttc ttactgcctt cgattggatt tctagccttg
     6361 gtaaggttct tcgtttacct acgaattaaa ccacataatc caccgcttgt gcgagtcccc
     6421 gtctattcct ttgagtttta gccttgcggc cgtacttccc aggcggatca cttaacgcgt
     6481 tagcttcgcc tctcagaggg tcgatactcc gaaaagctag tgatcatcgt ttagggcgtg
     6541 gactactggg gtatctaatc ccattcgcta cccacgcttt cgtgcttcag cgtcagtact
     6601 gtaccagtgt gctgccttcg cttttggtgt tccccatgat atcaacggat ttcaccccta
     6661 caccatgagt tccgcacacc ccttacatac tcaagcactg ccgtatcaat cgcgtctccg
     6721 aggttaagcc ccgggctttc acaattgact aacagcgcaa cctacgcacc ctttacgccc
     6781 aataattccg gataacacta ggggcctctg tattaccgct cctgctggca cagagttagg
     6841 agccccttat tcatgaggta ccgtcaataa acttcttccc tcataaaaga cctttacacg
     6901 ccgaagcgct tcatcaatca cgcggcatcg ctccatcaga ctttcgtcca ttgtggaagg
     6961 ttcttgactg cagccacccg taggtgtatg ggccgtgtct cagtcccatc ggtgggggtc
     7021 aggctctcac ctcccctacg cgtcatagcc ttggtaagcc attaccttac caactagctg
     7081 atacgtcaca ggccgctccc caagcgataa atctttaccc cggagggact atcgcgtgtt
     7141 agcacacctt tcggtgcgtt attcgcgact cgggggtgcg tacctatgtt ttactacctc
     7201 gtctgccact aaccagcaaa ccacatccac cactcaattc cccttgtatt gctacttggg
     7261 gtctcttgta gcttccgtgg actgattcgt tcgacttgca tgccttatcc atgccgccag
     7321 cgttcatcct gagctaggat caaactctca ttaaaagctt tcgcccgaag gcaaaaaact
     7381 agaacgaggc aaaagaaatg tgaatatgtt cttttgcttt actttaatgt aaccgcccgc
     7441 agtagctcat gctccgggcg ttggaataaa cctttgagaa ctcgcattct ctacattctc
     7501 gatttgcttt tcaaaaaaca aacccagggc tttcccacag gtagaaggct attatataca
     7561 tcggaaaagc attgtcaacc gctgaaaacc ccctggattt cagggggtaa taaccttatt
     7621 tatgaacttt tttgcgactc ttaggcaatt caatatcaaa atgcgccgtc cttacttgta
     7681 cttgctcaaa ttctccatcc attttttttg gattctgact attaacatga taccgcacag
     7741 atccggtaat acgagtgggc gtaaaatact ctattgtccc attttctttt cttgtttcta
     7801 cagttacaac cttaaacact tgaaaaaatc gaggactgcc ggaaaattct tcactccgat
     7861 atgtattctt aatctgtact ggaatttctg ttgtatcgct caccttttta ccgttaactc
     7921 gttttttggt tacatgttgc tcagaggtca taactgaagc acgtcgaatc ctgagatcat
     7981 ttttgccgcg cgaagcaggg tctcgcatca gttttactcc attattttca tgtgcgaacg
     8041 aaaatatcat ccgcttacca aaaacaatac atccattttc aggggttttc tctggtccaa
     8101 aaagaacaga gtcttcgggg atgttattga atacgacata gagccggtac cttttggctc
     8161 ccacctttgt aacaaagaat tccggttcaa cagctaat
//
LOCUS       DATIBQ010000013         7095 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U16294
            k141_2108181, whole genome shotgun sequence.
ACCESSION   DATIBQ010000013 DATIBQ010000000
VERSION     DATIBQ010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935789
            Sequence Read Archive: SRR25158331
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 7095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:58:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 637
            CDSs (total)                      :: 603
            Genes (coding)                    :: 601
            CDSs (with protein)               :: 601
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7095
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2108181"
                     /isolate="SMAG_U16294"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..373)
                     /gene="rplJ"
                     /locus_tag="VLB02_00345"
     CDS             complement(<1..373)
                     /gene="rplJ"
                     /locus_tag="VLB02_00345"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HSE56527.1"
                     /translation="MAISKDKKKEIVSSFETIINSAQSIVFVQFSKLTVKDANSLRRK
                     LQASEVGYKVGKKTLLKRVLATQGYTGTLPDLPGEIAIAYGTDALAPARELYEFAKEH
                     KGAVSIVGGVFEGMYKTKEEMM"
     gene            complement(578..1072)
                     /locus_tag="VLB02_00350"
     CDS             complement(578..1072)
                     /locus_tag="VLB02_00350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56528.1"
                     /translation="MEQNMNKNQLPAGKPKSSKTLWFSMLGALLLIILVVAAPRLKRG
                     SDIEAPALDDTELGAGAELSPAPESAAPKVLSRTEAAAKYAGQRMEFSLDCKAAPATV
                     AVKQGATVMLDNQSNKPHTVALAGKLYAIGAYKYRTVTLATLGSFPVACDEAQNAGAF
                     IVQE"
     gene            complement(1133..1921)
                     /locus_tag="VLB02_00355"
     CDS             complement(1133..1921)
                     /locus_tag="VLB02_00355"
                     /inference="COORDINATES: protein motif:HMM:NF015666.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56529.1"
                     /translation="MNIEKIAIVGMGVVGNGMDQLLKKRFTTVPYDIDGSKEAVQTCD
                     MAIVCVPTPMRDDGSCDISAVEEVVSWLETPLILIKSTIPPGTTRMLREKYQKKINFS
                     PEYMGESAYFTPYWKYPDPKRAETHTFVIVGGNEASDILNVFMKVMSVDTHYVVATAE
                     EAELTKYMENTFFATKVSFCNEFYDIAKGYGVDYKRLRELWLLDSRINPNHTLVFEDN
                     RGWGGKCYPKDVHAIIKAAENIDYDPLLLKAVVASNKKVRKGDR"
     gene            2016..3311
                     /locus_tag="VLB02_00360"
     CDS             2016..3311
                     /locus_tag="VLB02_00360"
                     /inference="COORDINATES: protein motif:HMM:NF014453.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HSE56530.1"
                     /translation="MNSSRITTLLVGDTLCFAAALLLMIAAETSGTPQTGIVAFYAQA
                     FAVPLFLFLIINYITNLYDIQSTVATPRSIGRFALAAVLGGGITALFFYIFPKFGISP
                     KTNLVILAAGFFILLTVWRRIFFHLFARSLARRIFFIGESAETTHLQEALTAHPHLGT
                     TVGIAKTLDAYLAADLPVDMLVIAKDIGVQEVERAAHTAVSIITVRGAFEELFGRIPL
                     AHFTAEDAFSLLEQKSQERKTIFYRLFEIVIATSVLIISSPFLLVAALAILIEDGRPI
                     FIQQKRVGEHGNIFTVYKFRSMKALAPDGSAEIAGAQWAEKKDNRRTKVGNILCTLHI
                     DELPQMWNIIRGDIALVGPRPERPEFVIMLEKEIPFYFLRHAIKPGFTGWAQIKYRYA
                     RTIEDSREKFEYDLYYLRHRSLLLDIGILAKTIQIIFTH"
     gene            3320..4114
                     /locus_tag="VLB02_00365"
     CDS             3320..4114
                     /locus_tag="VLB02_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012685920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF817 domain-containing protein"
                     /protein_id="HSE56531.1"
                     /translation="MKTVQKWAAEFFVFGIKQAQSCIFAGSFFILLFLSNHISLFGLA
                     RYDFLFLASVLIQVILYATKLETKDEVKVIFLFHIIGLILELYKTNPLIGSWSYPENG
                     FFKIGTVPLYSGFMYAAVGSYISQAWKMFKLELKNYSSYLFSVILCALVYINFFTNHY
                     LPDARWILIPLIFIFFWNTRVYFTITDKRRYMPLSISFLLIAFFIWVAENISTYWGAW
                     EYPDQVHQWSLVSTSKVTSWFLMVIISFIIIAYLKHFKKEVLNQKL"
     gene            4170..5624
                     /locus_tag="VLB02_00370"
     CDS             4170..5624
                     /locus_tag="VLB02_00370"
                     /inference="COORDINATES: protein motif:HMM:NF015013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="HSE56532.1"
                     /translation="MNRIRKFLFTNTTLRQTLLKNTFWLAASTTVSRIIRSIVIIFAA
                     RLLGTEHYGFFTYAMSLAALFTIFADVGLSGLLTRELAKRSEKDEFYISTLSFTKLAL
                     IICSTLLIIIFGPLIAKFDEAKPLLVIIALLLAFDGLRSFIYSIIRSRNQMQSEAGFE
                     LATEACLTAFCLGALLIKPTALALAIGYMVGSGAGFLILAVSFRKSITRIFSRIKRQL
                     IIPILSTTLPFAIVGIFNVLMTNIDSVIIGVWNSAETLGLYGAAQRPMSLLYLLPGFL
                     GTSLFPIMSTLVKEGNTAQAQVLVRKAVLASILLATPIVLGGLLIAKPFINAIFGYSY
                     VGAVPTFKILLVTLLPIFPGIIFYYLLFAENRQKVFIVSSGIGAIINIALDFLFIPRY
                     GIAGSAVATLCVQLVVNTILFRAVRKHYVFNIKKEITKMFVALASMGTAVLALLSWST
                     PLLVIIPVGIIVYVGVLILLKTEFIIDIKKSFVA"
     gene            complement(5602..6714)
                     /locus_tag="VLB02_00375"
     CDS             complement(5602..6714)
                     /locus_tag="VLB02_00375"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HSE56533.1"
                     /translation="MKILYLITKSEIGGAQTHVLQLVEYFSGQGHTVAVMAYPGGPLE
                     ARVRAAGAKFYPNNFFSNSYNPFRIIRAMRVIQKTVEEFSPDLVHCHSSAASVVGRLV
                     LRGKVPTIYTAHGWAFTERAPFLRRHIAQIVERLVSRYTAKTICVSKADYELSLRNGV
                     GNEKNTVIVYNGISTRGDFFVRKERDMVTVLFVGRLAYPKEPLLLIEAFAALLEDSKK
                     KAELVFIGEGTLRTDAEKLVITHQLEQYVIFRGSVEPAVVIDAMRHADIFVLLSRHEG
                     FPMTILEAMSVGLPVVASNVGGIPEQVSAASGVLVENNPDSVAKALLVLIKNKSLRLA
                     LGDAARARIVEEFSLEIFLQKTEAVYKEVLMQQKTS"
     gene            complement(6711..>7095)
                     /locus_tag="VLB02_00380"
     CDS             complement(6711..>7095)
                     /locus_tag="VLB02_00380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56534.1"
                     /translation="ESVELKRVLIKYNPFFHPGIMVRRKVFDAVGFYDEAWRFAQDYE
                     LYFRVAKLFELANVPQVLLRYRETFGSITGSKNRKQIGFVLKAKRKAIREGQYSRIHY
                     FDLLHTYISWILPIWIKRFFKKIVQ"
BASE COUNT         1895 a         1745 c         1334 g         2121 t
ORIGIN      
        1 ccatcatctc ttctttggtt ttatacattc cttcaaacac accaccaaca attgaaaccg
       61 cgcccttgtg ctcctttgca aactcgtaga gctcgcgggc aggagccaga gcatcagtgc
      121 catacgcaat cgcaatctca cccggaagat caggcaacgt tccggtatag ccttgagtag
      181 caaggacacg cttcaagagc gtcttcttcc ctaccttata gcccacctca gaagcctgga
      241 gtttgcggcg gagactattt gcgtctttta ctgtcagttt tgaaaactgg acaaagacga
      301 ttgactgcgc actgttgatg atcgtttcaa aagaagacac tatctctttc tttttgtctt
      361 ttgaaattgc catactattt ttttacaaag taaactactg tgtagtgaac gagaatttcg
      421 acctcggcag gtctgccctc tggcagatta ccccctgttt aaacacaaag gacctactgt
      481 cttcaatcta cacaaacgcc actataccga acagtccagt aaaagtcaaa acccgccatc
      541 tacagcgggt tttgactttt ataccattcg ttaaaaacta ttcctgtacg ataaatgccc
      601 ctgcattctg cgcttcatcg caggcaacag ggaacgagcc gagtgttgca agcgttacgg
      661 tacggtactt gtaggcgcca attgcataga gctttccggc aagagcaacc gtgtgcggct
      721 tatttgactg attgtcgagc atgacagttg ctccctgctt tactgcaact gttgccggtg
      781 ctgccttaca atcgagcgaa aactccattc gctgccctgc atacttagca gcagcttcag
      841 ttcgagaaag tacttttggt gctgccgatt caggcgcagg tgataattct gcccccgctc
      901 cgagttcagt atcatcaagc gctggagctt ctatatcgct gccacgctta agccgcggtg
      961 cagcaactac taaaataatt aaaagcaatg ccccgagcat cgaaaaccag agtgtcttgc
     1021 tactctttgg tttccctgct ggtaactgat tcttattcat attctgttcc atatccgtga
     1081 attctatacc tactaccttt taaattgcaa tagtgactgt tttttaaaca atctacctat
     1141 ctccttttct caccttttta ttcgacgcga ctaccgcttt aagtaggagt ggatcatagt
     1201 caatattctc tgctgcctta ataattgcat gtacatcctt tgggtaacac ttacctcccc
     1261 accctctatt atcttcaaat acaagtgtgt gatttggatt aatacgcgaa tctaagagcc
     1321 ataattcacg gaggcgcttg taatcaacac catacccttt agcaatatca taaaactcat
     1381 tacaaaaaga aacttttgta gcgaaaaatg tattttccat atattttgtt aattctgctt
     1441 cctcagcagt tgcgaccaca taatgtgtat ctactgacat caccttcata aacacattaa
     1501 gtatatcgct tgcttcgttt ccaccaacaa taacaaaagt gtgtgtctct gcgcgcttag
     1561 gatcggggta tttccagtac ggcgtaaaat aggcactttc gcccatatat tcaggagaga
     1621 aatttatctt cttctggtat ttctcccgga gcattcttgt agtccctggg gggatagtgc
     1681 ttttaatgag aataagcggt gtttcaagcc agcttacaac ttcctccacc gctgaaatat
     1741 cacacgaccc atcatcgcgc attggggtag gaacacacac tattgccata tcacaagtct
     1801 gtaccgcttc ttttgagccg tcaatatcat acggaacagt ggtaaaacgc tttttgagca
     1861 attgatccat tccattccct actaccccca taccaacaat ggcaatcttt tctatattca
     1921 tattcgatca gtatactaca acgttctgtc aatatattta caatacaatc tattgccaaa
     1981 ataatatttc tgtattagaa ctaactaacc tacatatgaa cagctctcgc atcaccaccc
     2041 tccttgtcgg cgacaccctc tgcttcgctg ccgccctctt gctcatgatc gctgctgaaa
     2101 cgagtggcac ccctcaaacg ggcatcgtag ccttttacgc tcaagcattt gcagtaccac
     2161 tcttcctctt cctgattatc aattacatca ccaacctcta cgacatccag agcactgttg
     2221 ccacccctcg ttctattggt cgattcgcac ttgctgcagt actcgggggg ggtatcactg
     2281 ccctcttttt ctatatcttt ccaaagtttg gaatttcccc aaaaacaaat ctcgtaatac
     2341 tggcagcagg attttttatc ctgctcactg tgtggcgcag aatattcttt cacctgttcg
     2401 cccgctcact tgctcggcgc atatttttta tcggagagag tgccgaaaca acccacctcc
     2461 aagaagcgct taccgcacac ccacaccttg gcactactgt tggtattgct aaaacactcg
     2521 atgcatacct cgcagcagac cttccggtag atatgctcgt tattgcaaaa gacattggtg
     2581 tccaagaggt tgaacgcgcc gcgcacactg ctgtttcaat aattaccgtg cgcggcgcgt
     2641 ttgaagaact ttttggtcga atcccccttg cccactttac cgctgaagat gccttttcgc
     2701 tcttggagca aaaatcacaa gaacgaaaaa cgatctttta tcgattattt gaaatagtca
     2761 ttgccacaag tgtgctcatt atttcttcac catttctttt agtggcagcg ctggcaattt
     2821 taattgaaga tggtcgacca attttcatac aacaaaaacg agttggggaa cacggcaata
     2881 tttttaccgt gtataaattc cgcagcatga aagcgctcgc ccccgatggg tcagctgaaa
     2941 ttgcaggagc acagtgggca gagaaaaaag ataatcgtag aacaaaagtt ggaaacattt
     3001 tatgtacact gcacatcgat gaactaccac aaatgtggaa tatcatccgc ggcgacattg
     3061 cccttgttgg tccccgcccc gaacgacctg aatttgtaat aatgcttgaa aaagaaatcc
     3121 ctttctactt cctgcgccac gctataaaac ctgggtttac cggctgggca caaattaaat
     3181 accgttatgc ccgcaccatt gaagactccc gcgaaaagtt cgagtacgat ctctactacc
     3241 tccgccaccg gtcgctcctc ctcgatatcg gcatcctcgc caaaacaatc cagattattt
     3301 ttacccacta aagcagtaca tgaaaacagt acagaaatgg gcagcagaat tttttgtttt
     3361 tggcattaaa caagcccagt cgtgtatttt tgcaggatct tttttcatcc tccttttctt
     3421 atcaaaccac atttctctgt ttggcttggc acggtacgac tttctctttc tcgcctccgt
     3481 actgatccaa gtaatactgt atgcaacaaa acttgaaaca aaggatgaag taaaagtcat
     3541 ttttttgttt catattatcg ggctcattct tgagttatat aaaacaaacc ccctgatcgg
     3601 atcttggagt tacccagaaa atggcttctt taaaattgga accgtgcccc tctacagcgg
     3661 atttatgtat gcagcagtcg gcagctacat aagccaagcg tggaaaatgt tcaaacttga
     3721 attaaaaaat tacagtagtt atctatttag tgttatttta tgtgccttag tgtatataaa
     3781 tttctttacc aatcattatc ttccagatgc cagatggatc ctcattccgc tcatctttat
     3841 ctttttttgg aacacacgcg tctattttac tattaccgat aaacgtcggt atatgccgct
     3901 cagtatttcc tttctactta tcgctttctt tatttgggtt gccgaaaata tctctaccta
     3961 ctggggcgcc tgggagtacc ccgatcaagt gcaccagtgg agcctcgtaa gcaccagcaa
     4021 agtaacctcg tggtttttaa tggtgattat aagttttatc atcatcgctt atttaaaaca
     4081 ttttaagaag gaagttttaa atcaaaaatt ataatacttt ttttcagcaa aataatttat
     4141 gagacctggt gtatgatgag gaaacagtta tgaatcgaat aaggaaattt ttattcacca
     4201 ataccacgct ccggcagaca ctattaaaaa atacattttg gctcgcagca agcactaccg
     4261 tcagtcggat tattcgaagt attgttatta tttttgcggc tcgactgctg ggtacggaac
     4321 actatggttt ctttacctat gcaatgagtc tcgctgccct attcaccata tttgctgatg
     4381 tggggctttc aggtctcctt actcgggaac tcgcgaagcg gtcggaaaaa gatgagtttt
     4441 atatttcaac attatcgttt acgaaattgg cgcttattat atgctctact ttactcatta
     4501 ttatttttgg accattgatt gcgaagttcg atgaagcaaa accactttta gttatcattg
     4561 cactcctact cgctttcgac ggcttacgaa gcttcatcta ttccattatt cgatcacgga
     4621 atcagatgca gtccgaagcc ggatttgaac ttgcgactga agcctgcctc accgctttct
     4681 gcctgggagc actcctcatc aaaccgactg cgctggcgct ggcgatcggc tacatggttg
     4741 gcagcggagc aggttttttg atactcgctg tatcatttcg taaaagtatc acaaggattt
     4801 tttcgcgtat caagcggcaa ctcatcattc cgatcctgag cacgaccttg ccatttgcga
     4861 tcgtgggaat attcaatgtg ctcatgacca acattgactc ggtcatcatc ggcgtatgga
     4921 actctgcaga gacgcttggc ttatacggcg ctgcccaacg gccaatgagc ctcctctacc
     4981 ttctccctgg attcttgggt accagcctat ttcccattat gagcacactc gttaaggaag
     5041 gaaacaccgc acaggcacaa gtgctcgttc gcaaggcagt gctcgcttca atcctccttg
     5101 cgacccccat agtcctcggc ggtctcctta ttgccaaacc atttattaat gccatttttg
     5161 gatatagcta cgttggcgca gtgccaacat tcaaaatact tcttgttact ctgttgccaa
     5221 tttttccggg tattatcttt tactacttac tgtttgcaga gaatcgtcag aaagtgttta
     5281 ttgtctcgag cggtatcgga gcgatcataa acatcgcact ggatttcttg tttatccccc
     5341 gttacggaat tgcaggctct gctgttgcca cactctgcgt ccaactcgtg gtaaatacta
     5401 ttcttttccg agcagttcga aagcactatg tgttcaatat taaaaaagag attactaaaa
     5461 tgtttgtagc gcttgcaagt atgggaaccg ctgtgctagc actcctttca tggagtacac
     5521 cattactcgt tattatccca gtggggataa tagtatacgt cggggtactt atacttttaa
     5581 aaacagaatt tattattgat attaagaagt cttttgttgc ataagcactt ctttgtacac
     5641 tgcttctgtt ttttgtaaga aaatttcaag ggaaaattct tctacaattc gcgctcgcgc
     5701 agcgtccccc agagcaaggc ggagagattt atttttaatg agcactagga gcgctttggc
     5761 gacagaatcg gggttattct ctacgagtac acccgaagct gcgctaacct gctcggggat
     5821 accgccaaca tttgaggcga ctactggcaa tcccacactc atcgcctcaa gtattgtcat
     5881 cggaaaccct tcatgtcgtg aaagtaatac aaaaatgtca gcatgcctca ttgcatcaat
     5941 aacaacagct ggctctacag aaccacgaaa gataacgtac tgctctaact gatgtgtaat
     6001 aactaatttc tctgcatccg ttcgcagggt accctcacca ataaatacaa gttctgcttt
     6061 cttcttacta tcttccagca gtgccgcaaa tgcttcaata agcaagagcg gttcttttgg
     6121 gtacgcgaga cgcccaacaa aaagaactgt taccatatca cgttcctttc tcacaaaaaa
     6181 gtctcccctt gttgatatgc cattataaac aataacagta tttttttcat tccctacccc
     6241 attacgcagc gagagctcgt aatcagcttt agaaacacaa atggtttttg ctgtataccg
     6301 ggacaccaac cgctcgacta tttgtgcaat gtgtctcctt agaaatggtg ctcgctcggt
     6361 aaatgcccac ccgtgcgccg tatagattgt tggcaccttc cctctcagca caagccttcc
     6421 cacaacactc gcagctgagc tatgacagtg aacaaggtcg ggagaaaatt cttcaactgt
     6481 tttttgtatt actcgcattg ctctaattat acgaaatgga ttatacgagt ttgaaaaaaa
     6541 attgtttggg taaaatttag cccccgctgc gcgtacccgt gcctcaaggg gaccgcccgg
     6601 atacgccatc actgcaactg tatgtccctg gccagaaaaa tattcaacaa gttgaagtac
     6661 gtgtgtctgt gcacctccta tctctgattt tgtaattaaa tagagtattt tcattgcact
     6721 atttttttga agaaccgttt tatccatata ggcaatatcc agcttatgta tgtatgtaaa
     6781 agatcaaagt agtgaatcct tgaatattgc ccttctcgaa tcgcctttcg ctttgccttt
     6841 aagacaaacc cgatctgttt cctattctta ctcccagtaa ttgatccgaa tgtttctcgg
     6901 taacgaagta gtacctgcgg tacatttgct aattcaaata atttagcaac tctaaaatag
     6961 agttcataat cttgcgcaaa ccgccacgct tcatcataaa aaccgacagc atcgaatact
     7021 ttccgacgaa ccataatgcc agggtgaaaa aaagggttgt atttaatcaa tactcttttt
     7081 aactctacac tctca
//
LOCUS       DATIBQ010000014         4669 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U16294
            k141_1603021, whole genome shotgun sequence.
ACCESSION   DATIBQ010000014 DATIBQ010000000
VERSION     DATIBQ010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935789
            Sequence Read Archive: SRR25158331
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 4669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:58:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 637
            CDSs (total)                      :: 603
            Genes (coding)                    :: 601
            CDSs (with protein)               :: 601
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4669
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1603021"
                     /isolate="SMAG_U16294"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1264
                     /locus_tag="VLB02_00385"
     CDS             <1..1264
                     /locus_tag="VLB02_00385"
                     /inference="COORDINATES: protein motif:HMM:NF037016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HSE56535.1"
                     /translation="DGFISSYLWQQTAGPVAVNIALPTASSTTVSGLSVAGVYTFELT
                     VTDNQGATAKASTMVMVNPAPNNPPTTNAGADQTITLPTNSVTITGTASDPNGDALSY
                     LWAKVSGGTATISSPTALTTTVTGLTQGTYIFRLTATDSKGATGSDDMQVVVNAVLSN
                     VPPTANAGADQVVILPKNSTILSGSGNDTDGIIASYAWKKISGGSAVISSPSAAATNI
                     TGLKQGTYTFRLTVTDNKGANASDDVIVEVKKQSAQDTTAPAVTVTSPLQGATVSGTT
                     VAIAAEASDNVGVTEVRFVLDYSTNLITMTTAPYAFVGDSTTIADGTHTLQAIAKDAA
                     GNVKTSTAVSFTVNNTAAPVTQAQVTVMSTLNVRDAAPDGTVIGIVDNLTIGNVLEIQ
                     NLPDDSVWVKMQFPTITGWVSAKYLKQL"
     gene            complement(1336..1527)
                     /locus_tag="VLB02_00390"
     CDS             complement(1336..1527)
                     /locus_tag="VLB02_00390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56536.1"
                     /translation="MENNNVYNLLNQLTQESKSLWRIKKNYLKEAKSKTLKDFWTKLA
                     KDKESHIKELKNLIKGEMK"
     gene            complement(1567..3321)
                     /gene="thrS"
                     /locus_tag="VLB02_00395"
     CDS             complement(1567..3321)
                     /gene="thrS"
                     /locus_tag="VLB02_00395"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HSE56537.1"
                     /translation="MEQNLEHLRHSLAHLLGAAVLELYPTAKLTLGPAIDDGFYYDVK
                     FEEPIGINDLPKIEKKMKALLPAWKNVSSREVSKEEAIDFYKENEFKKELIEEIAARG
                     ELITLYTMGSFIDLCRGGHVDDLSTIDPDSFKLDRVAGAYWRGNEQNPMLTRIYGLAF
                     ESKEVLAEYLHNREEAEKRDHRKLGKELGLFTFSPLVGAGLPIWLPHGATVRRILERF
                     IVDTELEWGYLHVNTPDIAKLDLYRTSGHYPYYKDSMYAPIQIDDEEFMLRPMTCPHH
                     FQVFSDSPKSYRDLPMRIAELAKLYRYEASGELTGLVRVRGFCLADAHIICADAEQAT
                     AEVGKALDLIEYVAGVFGLASGVDYWYRLSLGDRSDEKKYYKNDEAWDTGEALLRSIL
                     KNRSARFDEAPGEAAFYGPKIDIQMKDVRGKENTAFTVQYDFCMPDRFNLEYTDKDGT
                     KKRPLVIHRSSIGAIERVMAFLIEHYAGAFPTWLSPVQVAILPVGGSQQEYAAALFEK
                     LKNKGVRAELLTDDSLGKRIREAKTKKIPYQIIVGDKEKDTETITVEGRNSLKLEQIP
                     LQQFTERIGEEINTRKDQ"
     gene            3403..4005
                     /locus_tag="VLB02_00400"
     CDS             3403..4005
                     /locus_tag="VLB02_00400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56538.1"
                     /translation="MDIYPQYGGLFLSTEQSLKKYQQTVPRHTLHFAEVTSLRRTYAN
                     IFTTFDKKKEGILLTPYSRKEKIRIKKIKEEEERHNTLRRGSLKIFGLDVDEYEQLGG
                     PLFYNQQNEAELPNRRQYPKFMHVIYKFQGVVKDEQDQLFPGPNGLPVWVHYSLLPLI
                     FEVTRETQFGVRKHSQFEAYALLKKWLGEQMVSTEFCEPW"
     gene            complement(4076..4609)
                     /locus_tag="VLB02_00405"
     CDS             complement(4076..4609)
                     /locus_tag="VLB02_00405"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HSE56539.1"
                     /translation="MKRFAPTRPVLLEEIDEAKAKKLHISSSISASVIIFVPRIENGI
                     PEILLVDEWSLFDSDSKLKAPAGKILSGENFLDAAKRKLPIETGLKLLSAKFCFGGEF
                     SSTTNQRHYKIFLLGDKVEGEVAWGGDGKISRKIWVSVEKLEGKINLAQQFGLHYMVE
                     VAKTLNEEYGMAFMNSY"
BASE COUNT         1264 a         1013 c          996 g         1396 t
ORIGIN      
        1 agatggattt atcagtagct atctatggca gcaaactgct ggtccagttg cagtaaatat
       61 tgctttgcca accgcaagca gtactacagt aagcggcttg tcagtagcag gagtgtatac
      121 ctttgagctg accgttaccg ataatcaggg agcaactgca aaagcaagta ctatggttat
      181 ggtaaatcct gcgcctaata atccaccaac cacaaacgcg ggggctgacc aaaccattac
      241 tttgccaacg aactcggtaa ctattaccgg aacagcaagt gatcctaatg gcgacgcact
      301 atcttatctc tgggctaaag tttcaggagg tactgcaaca attagttcac caaccgcact
      361 cactactacc gtaacagggc ttacgcaggg cacatacatc tttcgtttga ctgcaactga
      421 tagcaaaggc gcaaccggaa gtgatgatat gcaggtggtt gtcaacgctg tcctttcaaa
      481 tgtaccgccc actgcaaatg caggagctga tcaggtagtt atcctaccaa aaaactcaac
      541 tatcttaagt ggttcaggaa atgatacaga tggcattatt gcaagctacg catggaaaaa
      601 gatttcaggt ggctctgcag tgattagttc gccatctgct gctgcaacaa atattaccgg
      661 acttaaacag ggaacctata cctttagact caccgttacc gacaataaag gagcaaatgc
      721 gagcgatgac gttattgttg aagttaaaaa gcagtccgca caagatacca ctgctccagc
      781 tgttacggtt acttctccgt tacagggtgc tactgtttca ggtactacgg tcgcaatagc
      841 tgcagaagct tctgacaatg ttggagtaac tgaagttcgg tttgtccttg attacagtac
      901 taacttaatc actatgacta ctgctccata cgcatttgta ggtgattcaa ccactattgc
      961 tgacggtaca cataccttac aggctatcgc aaaagacgca gccgggaatg taaaaacatc
     1021 taccgcagtt tcatttacgg tgaacaatac agctgctcca gtaacacagg cgcaggttac
     1081 agtcatgtct acactcaatg ttcgagatgc agcgccagat ggtactgtta tcggcattgt
     1141 tgataatctc actattggta atgtgctaga aattcaaaat ctccccgatg attcagtgtg
     1201 ggtaaaaatg cagttcccca ctattactgg gtgggtttcg gcaaaatatc tgaaacagct
     1261 atagtatagg aattccgatc cttaaagaat aaaaaaccgc caaatttggc ggttttttat
     1321 attactatca acttcttact tcatttctcc cttaatcaaa ttctttagct cttttatgtg
     1381 gctttcttta tcttttgcca gttttgtcca aaaatctttc agcgtttttg attttgcttc
     1441 tttcaagtaa tttttcttaa tgcgccacag gctttttgat tcttgggtga gctgattgag
     1501 taaattgtat acattattat tttccatatg ctcaagtgta gcacacggta tacgagcagc
     1561 cgcttgctac tgatcttttc tcgtgtttat ttcttctcct attcgttcgg taaattgttg
     1621 gagtggtatc tgctctagtt ttaggctgtt tctcccctca actgttattg tttctgtatc
     1681 tttttctttg tctccgacaa taatctggta ggggattttt tttgtttttg cctcgcggat
     1741 gcgcttgccg agactgtcat cagttagaag ttctgctcga actcctttgt tttttagttt
     1801 ttcaaatagt gccgctgcgt actcctgctg cgatcctccg acgggcaaaa tcgcaacctg
     1861 caccggcgag agccaggtag gaaacgcgcc ggcgtagtgc tcgatcaaga atgccatcac
     1921 gcgctctatg gcgccaattg aagatcggtg gataacaagt ggccgctttt ttgtaccatc
     1981 tttatcggtg tactcaaggt tgaatcggtc gggcatgcag aagtcgtact gaactgtaaa
     2041 ggcggtgttt tcttttccgc gaacatcttt catttgaata tcaatttttg gtccatagaa
     2101 tgcagcttct cccggtgctt cgtcgaaacg ggcacttcgg tttttcaaaa tgctgcgcaa
     2161 tagtgcttcg ccggtatccc acgcctcatc atttttgtaa tatttttttt cgtcacttcg
     2221 gtcaccaaga gagagtcggt accagtaatc tacccccgat gccaagccga acacgccggc
     2281 aacgtactcg atcaagtcga gtgccttgcc tacttctgcg gttgcttgtt ctgcatcggc
     2341 acaaataata tgagcatctg ccaggcaaaa gccgcgtacg cgcactaatc ccgtgagctc
     2401 gcctgaggct tcataacggt agagctttgc cagttcggca atgcgcatgg gtaaatcacg
     2461 gtagctcttt gggctgtctg agaaaacttg aaagtggtgc gggcaggtca tcgggcgaag
     2521 catgaattct tcatcatcaa tttgtatggg tgcatacata gaatctttgt agtacgggta
     2581 gtgacccgag gtgcgataga gatcaagttt tgcgatgtcg ggtgtattca catgcaggta
     2641 gccccactca agctcagtgt ctacaataaa tcgctctaaa atacgccgca cggttgcgcc
     2701 atgcggaagc caaataggaa gaccggcacc aactaaggga gaaaaggtaa agagaccgag
     2761 ttctttgcca agtttgcggt ggtcgcgctt ctcagcctct tcgcggttat gcaggtactc
     2821 cgcgagcact tctttcgatt caaaggcgag accgtagatg cgggtgagca tggggttctg
     2881 ttcgttcccg cgccagtagg cgccggcaac gcggtctagt ttaaatgagt ctgggtcgat
     2941 agtgcttaag tcatcaacgt gtccgccgcg gcagagatct ataaaagatc ccatagtgta
     3001 cagagtgatt agttcgcctc gcgcggcaat ttcttcaatc agttcttttt taaactcatt
     3061 ttctttataa aaatcaatcg cttcttcttt tgaaacttct cgtgatgaaa catttttcca
     3121 ggcagggagg agtgctttca ttttcttttc aattttagga agatcgttta ttccgatcgg
     3181 ctcttcaaac tttacatcgt aatagaagcc gtcatctatt gccggaccca gggtgagctt
     3241 tgcagtaggg tagagttcga gtacggcagc acccagtaag tgggcgagtg agtgacgcag
     3301 gtgttccaga ttttgttcca tccaaaaact atagcatttt caaagggttt cagagagttg
     3361 acaaaatcta aatatttgtt atttttacaa aaaagattct ttatggatat atatccgcag
     3421 tacgggggtt tattcctatc aacggaacaa tctctaaaaa aataccaaca aacagtacca
     3481 aggcacacgc tacactttgc cgaggttact tctctgaggc ggacatatgc aaatattttt
     3541 acaacatttg ataaaaagaa agaaggtatc cttcttaccc cttactccag gaaggaaaaa
     3601 ataaggataa aaaaaatcaa agaggaagag gaaaggcata ataccttgcg aaggggatct
     3661 ctaaaaatat ttggtcttga tgtagatgag tatgaacaat taggaggacc gcttttctac
     3721 aatcagcaga atgaagctga gctgccaaat cgtaggcagt accctaaatt catgcacgtt
     3781 atctataaat ttcaaggggt ggtgaaagat gagcaggatc aactttttcc tggaccaaat
     3841 ggtttaccag tatgggtaca ctatagtctg cttcctttaa tttttgaagt aacgagggaa
     3901 acacaatttg gtgtccgaaa acattcccaa tttgaagctt atgcactttt aaaaaaatgg
     3961 cttggtgagc aaatggtatc taccgagttt tgtgaacctt ggtaacgagt tggtataaac
     4021 aataagccgc ctatcaatat gataagcggc ttttaatttc tcttgaatga aaaagtcagt
     4081 agctattcat gaaggccatc ccgtactctt cattcaacgt ttttgctacc tctaccatgt
     4141 agtgtaaccc aaattgttga gccaaattga tttttccttc aagtttttct actgataccc
     4201 aaatttttct ggagattttt ccatcccctc cccaggcaac ttcaccttct actttatctc
     4261 ccagcaagaa aattttatag tgccgctgat tggttgtgga tgaaaattca ccaccaaagc
     4321 agaattttgc tgaaagtaat ttcaatcctg tttcaatcgg taactttctt ttagcagcat
     4381 ctaaaaaatt ttcacccgaa agtattttcc ctgcaggtgc ctttagtttt gagtcagagt
     4441 caaaaagact ccactcatcc acgagaagaa tttctggtat cccgttttct atgcgaggga
     4501 caaaaataat tactgatgct gagatactgc tactaatgtg cagttttttt gcctttgctt
     4561 catctatttc ctccagaagc actggtcttg ttggggcaaa ccttttcatt tttaaaattt
     4621 tttttatgtt cgaaacataa aattatgttt gaaattatat tagcacata
//
LOCUS       DATIBQ010000015         9331 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U16294
            k141_1769849, whole genome shotgun sequence.
ACCESSION   DATIBQ010000015 DATIBQ010000000
VERSION     DATIBQ010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935789
            Sequence Read Archive: SRR25158331
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 9331)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9331)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:58:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 637
            CDSs (total)                      :: 603
            Genes (coding)                    :: 601
            CDSs (with protein)               :: 601
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9331
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1769849"
                     /isolate="SMAG_U16294"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1228)
                     /locus_tag="VLB02_00410"
     CDS             complement(<1..1228)
                     /locus_tag="VLB02_00410"
                     /inference="COORDINATES: protein motif:HMM:NF028500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HSE56540.1"
                     /translation="MRALSDTLDSIVRLDAHTRAGLAKLQLHTIVDLLYHFPTRYADA
                     AEFKSIALLTEGELVTVSGVIKKIQSKKSFRTRVPMSEAVVADPSGTIRVLWFNQPYL
                     ARMYPEGTTVTLSGKATLQNKKLVLINPDINKQKLLSIDSHDSLFGSNQNEQLSPVYP
                     ETKGISSRWLQHTIARALAALPELADPLPEFLRTAYNLPTLRTALVWIHTPRSRDDAL
                     IARKRFSFEEIFILQLARLSEREQLKALHAYEISTNHAELERFINTFPFPLTSAQKKS
                     IEHIVSDLEQHAPMSRLLEGDVGSGKTAVAAAASFITVHNRPLKRPEGAFGRPSQSDG
                     LAERQTFGNLQVAYMAPTELLATQHFESFIEYFRGSGIAIALITGSGCRKFPTKVASS
                     KTPWTTISRTQLSKWIA"
     gene            complement(1249..1614)
                     /locus_tag="VLB02_00415"
     CDS             complement(1249..1614)
                     /locus_tag="VLB02_00415"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSE56541.1"
                     /translation="MKKILIVEDDTFLKNLESSKFTREGFEVMPATTSEDVARLIAQT
                     VPDVVLLDLVLPGVDGFGILEMLKKNESTKHVPIIVFSNLSDEKDVIRAKDAGATEFI
                     VKANYTLDEIAQKIKTIVG"
     gene            complement(1623..2390)
                     /locus_tag="VLB02_00420"
     CDS             complement(1623..2390)
                     /locus_tag="VLB02_00420"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HSE56542.1"
                     /translation="MSEQELLAEVEQLKTANQNKADLISISAHELRTSLSAVKWLLKM
                     LVDEDFGKLSDEQRSLLERASLSNDRMIALVNDVLTLNHTDDSTINYKIELIDMVQLV
                     DEVVFDFTSEGFKKGVEIVFIKPSERLSVSGDKLRLRVVMQNLLENAIKYSERGSRIS
                     IFLTPAGENVQLTVKDSGIGIPPDEQQNIFNKFFRASNAVKKESVGSGLGLYTTKRII
                     EVHKGTITFETEQGKGTSFMVTLPRAASASAPAQEGV"
     gene            complement(2395..2880)
                     /locus_tag="VLB02_00425"
     CDS             complement(2395..2880)
                     /locus_tag="VLB02_00425"
                     /inference="COORDINATES: protein motif:HMM:NF024953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HSE56543.1"
                     /translation="MKPSKTKKIIGTLILVLVSLFLLFSAFGKLTAHPTALEMFAALH
                     IPYLRIPLGIIEALVAVLLVIPRTNRIAVLITSGFLGGAILAELFFGMTGAFAGTILL
                     LVWIGSALRHHTCYCGCANCQQCHEHCSDCKTGTCSLHPQGEACNCKPGCVCVKGKCT
                     C"
     gene            2996..3997
                     /locus_tag="VLB02_00430"
     CDS             2996..3997
                     /locus_tag="VLB02_00430"
                     /inference="COORDINATES: protein motif:HMM:NF013428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HSE56544.1"
                     /translation="MEKQTVIPNFGSYQFPFQKSHYSGKVREVYTTMEGYIAMISTNR
                     ISAFDVVFPEPIPYKGAVLNLIAAYFLEETKDICPNWLREVLYPNVSVGVACEPIKVE
                     VIVRGNMCGGLWRKYKDKKPIVWNLPYNLYEITPDIRENDFLPFPIITPTTKAENGHD
                     EDITEEEIISQGLCTKELWDEIEAVAHKLFAKGVAMAESRGLILADTKYEFGIWNGKL
                     CLMDELHTPDSSRYFYAEGFPERQKNGEPQKQLSKEFVREWLMERNFMGEEGHVRPEL
                     PDEFIKEVSKRYISLYTVLTQKPFPYAAADAATEEAVYEAVRLSFDQQRLKDKVPEN"
     gene            complement(4094..4564)
                     /locus_tag="VLB02_00435"
     CDS             complement(4094..4564)
                     /locus_tag="VLB02_00435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56545.1"
                     /translation="MKKLILLAMVFIASLTSCHKEISEPTPPIQSGIVKTDNPPASLF
                     SSTPYGNTRYCWIDSGSINQSRAQGQGDLCSWDNYSGTWYDCGLWQLMPVRKYWGIIN
                     GVIYLSESKDWYAISPNIFLKAYKIVQSRKTGIGELYAGYQNPIGTPFTIGHVQ"
     gene            4775..4859
                     /locus_tag="VLB02_00440"
     tRNA            4775..4859
                     /locus_tag="VLB02_00440"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4809..4811,aa:Ser,seq:gct)
     gene            5180..5770
                     /locus_tag="VLB02_00445"
     CDS             5180..5770
                     /locus_tag="VLB02_00445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56546.1"
                     /translation="MKITHDHLFGHTPFFTDSYIRQNGRIFPWGGFRTYNNIVPKFED
                     QTKDEGGMWAWSEKLIPYGSPRYYLHMENNYLYVVPQGRCKAVEVLALAGDVYVPSYD
                     IVTVTKNRTLYSPENHGKSFDFGHHFQISGVVPGTELILLTLEDDGAMERFCTASHYR
                     LFTKEGTAVPFMFKGVEEMKLLGNPKEGKYTFHILN"
     gene            complement(5801..6406)
                     /locus_tag="VLB02_00450"
     CDS             complement(5801..6406)
                     /locus_tag="VLB02_00450"
                     /inference="COORDINATES: protein motif:HMM:NF014038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class IV adenylate cyclase"
                     /protein_id="HSE56547.1"
                     /translation="METEVEAKFPNIDITKIRAQLETLGATLEHPEILMRRKNFDYED
                     WRLQKIGGWIRVRDEGNKVTMSYKQLNDRTLHGTQEVNLVVDDFDKACNFLSSIGLQE
                     KSYQETKREKWLYKNVEITIDTWPWVPPFIELEGPTEEMLKSIAKDLELDWNQAMHGS
                     VETIYQMHYNFTEEEIDHWDSITFIPEPEWLLIKKKPTLKK"
     gene            6576..6662
                     /locus_tag="VLB02_00455"
     tRNA            6576..6662
                     /locus_tag="VLB02_00455"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6610..6612,aa:Ser,seq:act)
     gene            6753..6992
                     /locus_tag="VLB02_00460"
     CDS             6753..6992
                     /locus_tag="VLB02_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018645072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HSE56548.1"
                     /translation="MYYVYILKCADRNLYTGCTNNLKERIARHHSGHVPATKERRPLE
                     LQNYVAFKDKHRAFEFELYLKSGSGRAFCKKHFDN"
     gene            7056..7730
                     /locus_tag="VLB02_00465"
     CDS             7056..7730
                     /locus_tag="VLB02_00465"
                     /inference="COORDINATES: protein motif:HMM:NF016300.4"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HSE56549.1"
                     /translation="MDRLKNNWVLVLGGVLTLCALVFFFSANSFSKQGEYVEVKGLSE
                     RIVKADTAIWSMTFEVKSNSIDSLYADIEKNIRSIKTFLKQKGFEDAEISVAPVNIYQ
                     DTYKEASFRYNSTSQVAVYTKKVDGAKAASNETLMLVKEGVVLSQNTIQFEFSDLNSI
                     KPDMLAEAIKNARETAAQFARNAGSRVGGVSRGNQGVFDITDKDPGSPEYKKIRLVST
                     LRFLLR"
     gene            7752..8465
                     /locus_tag="VLB02_00470"
     CDS             7752..8465
                     /locus_tag="VLB02_00470"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HSE56550.1"
                     /translation="MKTSVLIIAHNEERYIAECIRSVLRQTKKPDEVVLMVHNSTDQT
                     EAIAKTFPITVVPFSGSVGITYARIEGLKHLSGDVILCIDGDSYAKENWVAEMVATLE
                     KPGNVLVGSWVKYKGTIFGFLSNWFNKFWCVRQKKVERWIWGSSFAFWGRDKEYVREV
                     FEKSFALSKELGLSRNVDDYWLALFMKKRGRLEITNKTHVVSYTKEVSTWEVIRRNRE
                     NMQNAARMEQFLKNYSQRD"
     gene            complement(8472..8789)
                     /locus_tag="VLB02_00475"
     CDS             complement(8472..8789)
                     /locus_tag="VLB02_00475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56551.1"
                     /translation="MNIHSDFTSSAATRTYDATVTFANKKLIQGNAIYSSGDTLGKER
                     IVHCMITNQEWAATEIKDSCEFPFTTPATTKEGLELQIKNSELKPVNKTCGHDDLCYT
                     LVQ"
     gene            complement(8932..>9331)
                     /locus_tag="VLB02_00480"
     CDS             complement(8932..>9331)
                     /locus_tag="VLB02_00480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HSE56552.1"
                     /translation="LGEQMSIDESTSVLLIPIPPSKTYKIRRGFNQAHLIATIFSTAI
                     PHAKVVNIVTRVRNVAAQSSIKKKSARLRNMEGAFAAAAPPHNHTVAILVDDIITTGA
                     TMEAARKALKAAGWKTVYGFAVAYTPLREK"
BASE COUNT         2576 a         1847 c         2210 g         2698 t
ORIGIN      
        1 tcgcaatcca ttttgaaagt tgtgtgcgag agattgttgt ccacggtgtt tttgaagagg
       61 caaccttggt ggggaatttt cggcagccgc tgccggtgat gagcgcaata gcaataccgc
      121 tcccgcgaaa atattcgata aacgattcaa agtgctgcgt tgccaatagt tcagtgggag
      181 ccatgtatgc tacctgtaag ttgccgaatg tttgtcgctc ggctaaacca tcgctttgcg
      241 atggtcgccc gaacgcccct tcagggcgtt ttagtgggcg attatgcacg gtaataaatg
      301 aagcagctgc agcaacggcg gttttgccgc tcccgacatc gccctctaac aagcgcgaca
      361 tcggcgcatg ttgctctaag tctgaaacaa tgtgttcgat tgattttttt tgcgcgctag
      421 tgagcggaaa agggaaggtg ttgatgaacc gttcaagctc agcatggttg gtactgattt
      481 catacgcatg gagtgccttc agctgttctc gttctgaaag ccgggcaagc tggagaataa
      541 aaatttcttc aaacgagaat cgcttgcggg caatgagtgc atcgtcgcgg ctgcgagggg
      601 tatgaatcca caccagtgcc gtacggagcg ttggcaaatt gtatgcagtg cgaagaaact
      661 cggggagcgg atctgcaagt tcagggagtg cggcgagtgc gcgcgcgatg gtgtgttgca
      721 gccagcggga agagattcct tttgtttctg ggtacaccgg cgagagctgc tcgttttgat
      781 tgctgccaaa caggctgtcg tgtgaatcaa tcgaaagcag tttttgctta ttgatgtcgg
      841 ggttgatgag tactaatttt ttattttgga gcgtcgcttt tccggagagt gtaacggtgg
      901 tgccttcagg gtacatgcgt gccaggtacg gctggttaaa ccatagtacc cgaatagtgc
      961 cgctcgggtc tgcaactact gcttcactca tcggtaccct ggtgcggaat gattttttgc
     1021 tctgtatttt ttttatgaca cctgagacgg taacgagttc gccctcagtg agaagggcga
     1081 tcgatttaaa ctcagccgca tcggcgtaac gagtaggaaa gtgatacagg agatcgacaa
     1141 tggtgtgcag ttgcagcttt gctagtccgg cgcgggtgtg ggcatcaagc cgcacaatgg
     1201 agtcgagcgt gtcggagagt gctcgcatac aataaagtga atcaggagtt atccgacaat
     1261 cgttttaatt ttctgagcaa tttcgtcgag agtgtagttt gcttttacga taaactcggt
     1321 tgcgccggca tcttttgcgc gaatcacatc tttttcgtca gagaggtttg aaaatacaat
     1381 aatcggaaca tgttttgtgc tctcgttttt tttcagcatc tctaaaatcc caaagccgtc
     1441 cacaccaggg aggacaaggt caaggagcac cacatcgggt acggtttgag caataagccg
     1501 agcgacatct tcactcgtgg ttgcaggcat tacttcaaac ccttcacggg taaattttga
     1561 gctctctagg ttctttaaaa aggtgtcgtc ttcaacgatg agtatttttt tcatataggt
     1621 gattataccc cttcttgcgc tggggcggaa gcactcgccg ctcgggggag ggttaccata
     1681 aacgaggtgc cttttccctg ctcggtttca aacgtaatcg tacccttatg cacttcaata
     1741 attcgcttgg tggtgtagag cccgagccct gatccgaccg actctttttt taccgcattt
     1801 gaggcacgga agaacttatt aaaaatattt tgctgttcat caggcgggat gccgataccg
     1861 ctgtctttaa cggtgagttg cacgttttct ccggcggggg taagaaaaat agaaattcgg
     1921 ctgccacgtt cactatattt gatagcgttc tccaaaaggt tttgcatcac cacgcgcaag
     1981 cggagcttgt cgccagagac actcaagcgc tcggatggct taataaacac aatctcaact
     2041 ccttttttaa atccctcact ggtaaaatca aataccactt catcaaccag ctgtaccatg
     2101 tcgataagct caatcttgta gttaatagtt gaatcgtcgg tgtggtttaa ggtaaggacg
     2161 tcgttgacga gcgcgatcat ccggtcgttc gacagcgagg cgcgctctaa gagcgagcgc
     2221 tgctcgtcag agagcttgcc aaagtcttcg tcgacgagca tcttcaacag ccactttact
     2281 gccgagagcg acgtgcgcag ttcgtgcgcc gaaatagaaa taagatcggc cttattttgg
     2341 tttgcggttt ttagttgttc aacttcagca agaagctcct gttcgctcat atggttagca
     2401 cgtgcacttt cctttcacac aaacgcagcc gggtttgcag ttgcaggctt ctccctgtgg
     2461 gtggagtgag cacgttcctg ttttacagtc actgcagtgt tcgtggcact gttggcagtt
     2521 tgcacagcca cagtagcagg tgtggtggcg aagtgcagat ccgatccaaa ccaagaggag
     2581 tatagttcca gcaaatgcac ccgtcattcc aaaaaagagc tctgccaata tcgctccgcc
     2641 aagaaatcct gaagtaatca gtaccgcaat acggtttgtt cgaggaataa ccaaaagcac
     2701 agccaccagt gcttcgataa ttcccagcgg aatgcgcagg tacggaatgt ggagtgctgc
     2761 aaacatctcg agtgctgttg gatgagcagt cagcttccca aatgcactaa agagaaggaa
     2821 gagggaaaca agaacaagaa taagagttcc gataattttc ttggtttttg atggtttcat
     2881 tataaaaaat agctactaat aacccctgca gtatagcacc attccgcttt gactaaaggg
     2941 tgtttttccg gtagggtggg tgaaatttcc ttaatataac ttaaaacaaa aaaacatgga
     3001 aaaacagact gtaattccga attttggatc gtaccaattt ccttttcaaa aaagccacta
     3061 ctctggcaag gttcgagaag tttacacaac catggaaggg tacattgcta tgatcagtac
     3121 caaccgcatt tctgcatttg atgttgtttt tccggaacca attccctata agggcgcagt
     3181 acttaattta attgcggcat acttcttgga agaaacaaaa gatatctgtc caaattggct
     3241 ccgcgaggtt ctgtacccca acgtttcagt cggtgttgcc tgtgagccga tcaaggttga
     3301 ggtaattgtt cgagggaata tgtgtggagg tctgtggcgc aaatacaagg ataaaaagcc
     3361 tattgtatgg aatttaccct ataacctgta tgaaataact ccagatatcc gcgaaaatga
     3421 ttttttacca tttccaatta ttacgccgac gacgaaagca gagaatggtc atgatgaaga
     3481 cattactgag gaggaaatta ttagtcaggg tttgtgcaca aaagaattgt gggacgaaat
     3541 agaagcagtt gcccataaac tttttgcgaa gggtgtggca atggctgaga gtcggggttt
     3601 gatactggca gacacaaagt atgaatttgg aatatggaac gggaaactct gtcttatgga
     3661 tgagttgcat actcctgatt cttcacggta tttctatgca gaaggatttc ctgagcgcca
     3721 aaagaacgga gagccacaaa aacagctgag caaagagttt gttcgggagt ggctcatgga
     3781 gcgaaatttt atgggcgaag aaggccatgt acgacccgaa cttcccgatg aattcattaa
     3841 ggaagtttca aagcggtaca taagtctgta cacagtcctt acccaaaaac catttcctta
     3901 tgctgcagct gatgcagcaa ccgaagaagc ggtctatgaa gcagttcggc tttcatttga
     3961 tcagcaacgt ttaaaagata aggtgccaga gaattgaaca aacatattct tcttctttta
     4021 agtttaaata attatttaat atataaaaac acccgccatt atgcgggtgc cttgttttgt
     4081 gatttaaatc tttttattgg acatgcccaa ttgtgaacgg tgtacctata gggttctggt
     4141 acccagcata taattcacca atacctgttt ttctgctttg cacaatctta tatgctttca
     4201 aaaaaatatt gggactgatt gcataccaat ccttcgattc agataagtaa ataacaccat
     4261 ttataatacc ccaatatttt cttactggca taagttgcca gagtccgcaa tcataccagg
     4321 tgccactgta attatcccaa ctacagagat ctccctgtcc ttgtgctctt gactggttta
     4381 tcgagccgga atcaatccag cagtatcgag tgtttccgta gggtgtagag ctaaaaaggc
     4441 ttgccggtgg gttatcagtt tttacaatac ctgattggat aggtggggtt ggttcactta
     4501 tttctttatg acaagaagta agcgaagcaa taaaaaccat ggctaataaa atgagttttt
     4561 tcattaaact tgagtttttg ttttgaattt ttgattttaa acgaacgcta atctacatgc
     4621 taacatatta gcaatatatt gtcaatagta ccgcaaaaat acccatgaca aaagcactaa
     4681 agcggggtat tatggcggtg cgaggaattg gctaaaacat ccttaaactg ctttgcagca
     4741 ggatgttcgc ccaaactcgc aaagcgagtt ttagggagac gtgtcagagt ggtctaacgt
     4801 acctccttgc taaggaggca ggtgtcacag cctcgagggt tcgaatccct ccgtctccgc
     4861 tttcgcccga cgaagccttg gcgaagtcgg gattcgccgg cttcataacg ggctacggcg
     4921 gacacagtcc gctatagtgc gatgaagccg aacgaagtcg agtgtccggg tgggggcaaa
     4981 cgagaaaact gttttgctcg cgtgagggat gagaaaagct ttccgttatt tgtgaagagt
     5041 tttactctga acaaatcgga aaggtgtact gctcctgtaa ggagagaatc cctccgtttc
     5101 tgctgaatca ggtcattgtt gaatattgac aatattcaat attatgctac aataaaacca
     5161 aaccttaaaa tcttttgtca tgaaaattac acacgatcat ttattcggtc atactccttt
     5221 ttttacagat tcttatattc ggcaaaatgg aaggatattt ccatggggtg gttttcgtac
     5281 ctataacaat attgttccaa agtttgaaga tcaaacaaaa gacgaaggtg gaatgtgggc
     5341 atggagcgag aagctcatac cctatggttc acctcggtat tatctccaca tggaaaataa
     5401 ttatctgtat gttgttcccc agggaagatg caaggcagtg gaagtccttg ctcttgccgg
     5461 agatgtgtat gttcctagct acgacattgt tacagtgaca aaaaatcgta cactctacag
     5521 tccggaaaac catggaaaaa gttttgattt tggtcatcat ttccagatct ccggtgttgt
     5581 acctggaaca gaattaatat tactcactct tgaagacgac ggtgctatgg agcgattttg
     5641 cactgcaagc cactatcggt tatttacgaa agaaggaact gctgttccat ttatgtttaa
     5701 aggggttgag gagatgaagt tactgggaaa tccgaaagag ggtaaataca cttttcatat
     5761 ccttaactag gttgttaacg tattggcgta tttcaaaagc ttactttttt aaggtaggct
     5821 ttttctttat caacagccac tcaggctcag gaataaaggt tatcgaatcc cagtgatcaa
     5881 tttcttcttc ggtaaagttg tagtgcattt ggtaaattgt ttctacgctg ccatgcattg
     5941 cctggttcca atccagctct agatctttag cgatggattt taacatttct tctgttggcc
     6001 cttcgagctc aataaagggc ggtacccacg gccaggtatc aatagtaatt tcaacatttt
     6061 tatagagcca tttttcacgc tttgtttctt ggtacgattt ttcctgcaat ccgatagagc
     6121 tgagaaagtt gcaggcttta tcaaaatcat caacaactaa attaacttct tgagtgccgt
     6181 gtagtgttcg atcgttgagc tgtttatagc tcatggttac tttattacct tcatcgcgaa
     6241 ctctgatcca cccaccaatt ttctgcaagc gccaatcctc ataatcaaag ttcttcctcc
     6301 gcatcaggat ttcaggatgc tccaaagtag caccgagggt ttctagttgc gccctaatct
     6361 tagtgatatc tatattgggg aattttgctt caacttctgt ttccatagaa aaaatactag
     6421 caataaactg taaaaaagtc tttgaacctt tagttcttat ataatttatt ccacgaaaga
     6481 acaatttttt agggtggaat cgttaaagta tcatttttag atgagcagag aactaactaa
     6541 taaattgtgc tagaatagcc ttcacaataa ttattggagg attggatgag tggtttaaat
     6601 cagcggttta ctaaaccgcc gacccttaat cgggtccgtg agttcgaatc tcacatcctc
     6661 cgccttcgcc cgacgaagcc ttggcgaagt cgggattcgc cggcttcata acgggctacg
     6721 gcggacacag tccgctttag tacactcatg gtatgtacta tgtttacatt ttaaaatgtg
     6781 ctgatagaaa tttatataca ggctgcacca ataacctcaa agaaagaatt gcgcgccatc
     6841 acagcggtca tgttccagca actaaagaac gtagaccact agaattgcaa aactatgtag
     6901 cgttcaaaga caagcacaga gcatttgaat ttgagctgta tttaaagtct ggttcagggc
     6961 gcgctttttg taaaaagcat tttgataact aatatagaga tcattagaac ttagtgggaa
     7021 tagttttgtt aatggcaacg tagtagaata gcgtgatgga tagactaaaa aataactggg
     7081 tgctagtgct gggcggggtg cttacgctct gtgctcttgt attctttttc tccgcgaaca
     7141 gtttctcaaa acaaggtgag tacgttgaag ttaaggggct ctctgagaga attgttaagg
     7201 cagacacggc tatatggtca atgacgtttg aggttaaatc aaacagtatt gattctctct
     7261 atgcagatat tgaaaaaaat atccgttcga tcaaaacatt tcttaaacaa aaagggtttg
     7321 aagatgcgga gatcagcgta gctccggtga atatttatca agatacctat aaggaagcat
     7381 cattccgata caattcgacc agtcaggtag cggtatacac aaaaaaagtt gatggtgcaa
     7441 aagcagcatc aaatgagaca ctcatgctgg taaaagaagg ggtagtgttg agtcagaata
     7501 ctattcagtt tgaattctct gaccttaata gtattaaacc tgacatgctt gccgaggcaa
     7561 ttaaaaatgc tcgagaaaca gctgctcagt ttgctcgaaa cgctggtagt cgtgttggtg
     7621 gagtatctcg cggcaatcag ggggtatttg atatcaccga caaagaccct ggatctcctg
     7681 aatacaaaaa gattcgtctt gtttcgacgc tgcggtttct ccttcggtaa actggaggcg
     7741 ctgtgttgtg tatgaaaact tcagtcctca ttattgccca taacgaagag cggtatattg
     7801 ctgagtgtat tcgctcggtg ttgcggcaaa caaaaaagcc agatgaggtg gtactgatgg
     7861 tacataattc gactgaccaa acagaggcga ttgcaaaaac atttcctatt acggtggtac
     7921 ctttttctgg atcggttggt attacgtatg cacgaatcga ggggcttaaa catctaagtg
     7981 gcgatgttat tttgtgtatc gatggcgatt cgtacgcaaa agaaaattgg gtcgctgaaa
     8041 tggttgcaac gcttgagaag ccggggaatg tgcttgttgg ttcgtgggta aaatataaag
     8101 ggacgatatt tggttttctt tcaaactggt ttaataagtt ttggtgtgtg cgacagaaaa
     8161 aagtagaacg atggatatgg gggtcaagct ttgcattttg ggggagagat aaagaatatg
     8221 tacgagaagt ttttgaaaaa agttttgcgc tttcaaaaga acttggtctt tcacgcaatg
     8281 tagatgatta ctggcttgcg ctttttatga aaaaacgcgg gaggttagaa attaccaata
     8341 aaacacatgt agtaagttac acaaaagaag tttcaacttg ggaagtaatc agacggaata
     8401 gagaaaatat gcagaatgca gctcgtatgg aacaatttct caagaattat tcccaaagag
     8461 attagattta attactgaac taatgtgtag cataaatcat catgaccgca tgttttattg
     8521 accggtttta actcactgtt ctttatttgg agttcaagtc cttctttagt tgttgcagga
     8581 gtagtgaagg ggaattcgca agaatcttta atctcagtag ctgcccattc ttgattggtt
     8641 atcatgcagt gtactattct ttcttttccg agcgtgtcgc ctgaagaata tattgcattt
     8701 ccttgtatca gttttttatt ggcaaacgtt actgttgcgt catatgttct ggttgcagcc
     8761 gagctggtaa aatcactatg tatattcata gtaaaagagt ctggtagttg agtattattt
     8821 tttgatgaag catataacag aataagaatg gctaaaataa tagtaaccag tataaatttc
     8881 tttttcatag aaatattgta acagtttcaa aatcaaaaca tcatatgtat atcacttttc
     8941 acggagcggg gtataggcaa ccgcaaaacc atagacggtt ttccagccgg ctgctttgag
     9001 ggctttgcgg gcggcttcca tagtggcgcc ggtggtgatg atgtcgtcta caagaatggc
     9061 gacagtatgg ttgtgtggcg gcgctgctgc ggcaaaggca ccttccatat tgcgcagccg
     9121 ggcacttttc ttttttattg agctctgtgc ggctacgttt cgtacacggg ttacaatgtt
     9181 tactaccttt gcatgaggaa tggcagtgga aaaaatagta gcaatcaagt gcgcctggtt
     9241 gaacccgcgc cttattttgt atgttttgct gggagggata ggtatgagta gcactgaagt
     9301 gctttcatcg atactcattt gctcaccgag g
//
LOCUS       DATIBQ010000016         5994 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U16294
            k141_2093859, whole genome shotgun sequence.
ACCESSION   DATIBQ010000016 DATIBQ010000000
VERSION     DATIBQ010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935789
            Sequence Read Archive: SRR25158331
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 5994)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5994)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:58:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 637
            CDSs (total)                      :: 603
            Genes (coding)                    :: 601
            CDSs (with protein)               :: 601
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5994
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2093859"
                     /isolate="SMAG_U16294"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..844)
                     /locus_tag="VLB02_00485"
     CDS             complement(<1..844)
                     /locus_tag="VLB02_00485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56553.1"
                     /translation="MRVKLLLMVLSTAASLLFGSMETVLAQSSCPIVIDTSIVSVPGG
                     MQAYVAWDNPTGNDNNPGTGGKKSFMFIIQNGSTTCFSTCIDASDDGTFLSPVFTCTG
                     TPAMEFIAHTGNSTCQGTTCQIQILLPLRLISLTASKKSDGKVEISWNVAAESVNTTT
                     AFYLEESFNEFKDKTSIRTIAIMLPEVGKTGYRYTYLPTKENGLVSYRLRVHENMSEQ
                     YSKMLLLRLGREEQRASIGKTATDLLVYDALLNTLIQISTITGQKIFAIRSNSNVVKI
                     PLNNI"
     gene            995..2608
                     /locus_tag="VLB02_00490"
     CDS             995..2608
                     /locus_tag="VLB02_00490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56554.1"
                     /translation="MKKVATLFIALLLGVGITAQPSLWRPVFTPNASVTCWMQYGTKM
                     LFGGQFTLVNGQPMNHVVLYDPGTGLVSTVGTLPGPPLYMCWASDGRIRATGNFLVNG
                     EYYGAMIYDPGSNTWIVDPTTKFTGPCAAQQYIKFWLSLVTVNSDLVIGDLTEINTVP
                     VKQVVHIRNGNPENMFDVLDCWFCFPGKPVVEKVIIDGPTLKFMGAFSRLGKQYIDTT
                     WVTNFANFSGSSITPQNGQLYSVIRDHVFFKGNDAVTGSFFLPKPGAVEWDGSHWDPI
                     SGGVDINGNALAVICGRLFVAGKKSFFGGCSQAGIIASTDGNTGWKNESAGLNRFGDE
                     EITAFVRDTLRNEVYTLGSFKKSKGDTADYIAVRSFEACITNGPLAIKFTSFTAKKEG
                     LAVRLKWQIETDEQVTFSIERSTDGVHFSPIGTTSKYTFTDATLPASNTVFYRIVAKG
                     NTQKISPIVAVFLEKTNKVEFVMTPGIIHVRGGVGVIRVFTTTGVELLRKNISSENSS
                     FSHSLPYGVYILRFTSQQGVTTAKQAVVQ"
     gene            2843..3979
                     /locus_tag="VLB02_00495"
     CDS             2843..3979
                     /locus_tag="VLB02_00495"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HSE56555.1"
                     /translation="MKLDIPGSLRRFALPAALAALAVVVHSQPAQAYLFGRSASASEG
                     LDQAPALYNSQTMPLMKAGSQVTAEPLALEDGSFAASAGPLGVVYDTTVGGTISRYTV
                     RSGDTIETIAETFGVSKNTIVWANDLKRPPAPGGRAGGATISVGQELVILPVSGVRYV
                     VQKSGERIADIAKKFGGDAAEIEQYNDVDSTVLAKGTELIIPDGEIAAPEPKKSAPKK
                     VVQKETSTLFEEDHDDHEDETVSAKPKKLPTRVSVTVLKDPTGYFMVPLKTGTRTQKL
                     HGLNGVDLAAPTGTPIYAAAAGTVILVKGGNAWNGGYGNYVVIEHGNGTQTLYAHNSK
                     NLVSTGQTVAKGEQIALLGSTGKSTGPHVHFEVRGKGAKNPLAN"
     gene            complement(4369..5883)
                     /locus_tag="VLB02_00500"
     CDS             complement(4369..5883)
                     /locus_tag="VLB02_00500"
                     /inference="COORDINATES: protein motif:HMM:TIGR00368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HSE56556.1"
                     /translation="MNFSRLQSAQVSGLQPSIVTVETDLSRGLFLFSIVGLPDKAVEE
                     ARDRVLSALKHSVDVSPKTRNQKIVVSLSPAELKKEGAYFDVSIALGYLLSAGDIAFD
                     PAGKIFVGELALNGEVRPVRGILPIAEAARKAGCTELFIPAANSAEGALVSGITIYPV
                     ETLAQLIAHLKSEVLITPTVPQPITERPITARFDFGEVSGQLQAKRALLIAAAGGHNI
                     ALYGPPGTGKTMLARSFSGILPPLSEAEFLETAMIYSSVGLLDELWKTHVPFRSPHHT
                     ASHVAVVGGGAQLRPGEVTLAHNGILYLDEFPEFDRRVIEALREPLEDRAVTIARAKG
                     SAKYPSRFLLVAALNPCPCGYRGSATRACTCSAHDLVRYKKKLSGPIMDRIDLWVPVG
                     HIEYSALHERKETQESPALREQVSTARAQQHERFSKERLNADMQTKDIQQLVALPPEL
                     QTLLESYAKRLELSPRSYLRVIKVARTIADLEHAAAVTEAHLLEALQYRPKFEW"
BASE COUNT         1555 a         1335 c         1446 g         1658 t
ORIGIN      
        1 tgatattgtt cagtggaatt tttaccacat ttgaattcga tcgtatagca aagattttct
       61 gtccggttat tgtgctgatt tgaatgaggg tgttcaggag tgcatcatac accagtaaat
      121 ctgtcgctgt ttttccaata ctcgcccttt gttcttctct acctaagcgg agaagcaaca
      181 ttttcgagta ttgctcactc atattttcat gaacacggag ccggtaggat acaagtccat
      241 tctcttttgt aggcaagtaa gtgtacctat accctgtttt ccctacctca ggaagcataa
      301 tggcgatagt tcttattgag gttttatcct taaactcatt gaatgattct tcaaggtaga
      361 acgcagtagt agtattgact gactcagcgg ctacattcca gctaatttca acttttccat
      421 cactcttttt tgaagcagtg agagatatga gtcgcagtgg aagtaaaatt tggatctggc
      481 aggttgttcc ctgacaagtg gagttgccgg tatgggcaat aaattccata gctggtgttc
      541 ctgtgcaggt aaaaaccgga ctcagaaagg ttccatcatc actcgcgtca atacaggtac
      601 taaagcatgt ggtcgagccg ttctgaataa taaacatgaa gctttttttt ccacctgttc
      661 cggggttgtt atcattgccg gtaggattat cccaggctac atatgcctgc attccaccgg
      721 ggactgaaac aatagatgta tcgataacga tcggacacga agactgtgcc aacactgttt
      781 ccatgcttcc aaaaagcaag ctggctgcgg tactgagtac cataagtaaa agttttactc
      841 tcataatgat tgtgttttta aagtttagtt taacactaca ttaattattt ttttttgtca
      901 atggcttgtg ggtacgcacg ttttttgaca tttttacaaa attcggtata attctattga
      961 aatttaaatt gtttaacaac taaaaataag gtatatgaaa aaagtagcaa ctctcttcat
     1021 tgctctactt cttggggttg gcatcaccgc tcagccgagt ttatggcgac cggtatttac
     1081 tcctaatgct tccgttactt gttggatgca gtacggaaca aaaatgcttt ttggtggtca
     1141 gtttactctg gtaaatggtc aaccaatgaa tcacgttgtt ctctacgatc ctggtactgg
     1201 tcttgtcagt actgtcggaa cactgccggg tccaccgcta tatatgtgtt gggcttcgga
     1261 tggtcgcatt cgtgcgaccg gaaacttttt ggtaaatggc gagtattacg gagcaatgat
     1321 ttatgatcca ggtagtaata cgtggattgt tgatccaacg actaagttta ctggtccttg
     1381 tgctgcgcag cagtacatta aattctggct ttcgctcgta actgttaaca gtgatttagt
     1441 tattggtgat ttaaccgaaa taaacactgt tccggtaaaa caggtggtac acattcgcaa
     1501 tgggaacccc gagaacatgt tcgatgttct tgactgctgg ttctgcttcc ctggaaaacc
     1561 ggtggttgaa aaagtaatta ttgatggtcc gacccttaaa ttcatgggcg ctttcagtcg
     1621 tttaggaaaa cagtatatag acacaacctg ggtcactaac ttcgcaaact ttagtggaag
     1681 ttcaataact ccccagaatg gtcaactata ttctgttatc agagaccatg tttttttcaa
     1741 aggaaatgat gctgttaccg gttcgttttt tctccctaaa cccggtgcag ttgagtggga
     1801 tggttcacat tgggacccca tttctggagg tgttgatatt aatggtaacg cactcgctgt
     1861 tatctgtggc agactttttg tcgcaggaaa gaaaagcttt ttcggaggct gttcacaagc
     1921 aggtattatt gctagcactg atggaaatac gggctggaaa aatgaatccg ctggtcttaa
     1981 tcgattcggc gatgaagaaa ttaccgcttt cgtccgtgat acgcttcgca atgaggtata
     2041 tacactcggc tcttttaaaa agagtaaggg tgatacggca gattacattg cagtgcgcag
     2101 ttttgaggca tgtataacca atggtccatt agctatcaag ttcacttcat ttacggcaaa
     2161 aaaagaaggg ttggcagtga ggttgaaatg gcaaattgaa accgatgaac aggttacttt
     2221 ttcaatcgaa cgttctactg acggggtaca tttttcacct attggcacta cctcaaagta
     2281 cacgttcact gatgctacct tgcctgcaag taatacggtt ttttaccgga ttgtagctaa
     2341 ggggaatact caaaaaatct caccaattgt tgctgtcttc cttgagaaga ccaacaaggt
     2401 agagtttgtg atgactccgg gtattattca tgtacgaggc ggggttggcg tgatacgggt
     2461 gtttaccact acgggggtag aactgctcag aaaaaacatt tcttctgaaa acagtagttt
     2521 ttcgcactcg ctgccgtatg gagtgtatat tcttaggttc acttcccaac agggagtaac
     2581 tactgccaag caagcagtag ttcaataaaa ttcaatatcg ttaattcggt cggcttccgc
     2641 aggaagccga cctttttctt ttcctgcaga gcccttatgt ggcaagctta tttgtcattt
     2701 tttactacaa aaaataataa gatttttgtc aaatatagcc ctggtaaagg ttttttcgtg
     2761 aaaaatcgtt gactattgta tgtaactgtt tctttatact atttccctct ttcttcttgg
     2821 tatcattctt ctctgtagct ttatgaaact cgatattccc ggttccttac gccgttttgc
     2881 cttgcccgcc gcactcgccg cgttggctgt agtggtacac tcacagccgg cacaggcgta
     2941 tctgtttggt cgctctgcta gtgcaagtga ggggctcgac caggctccgg cactctataa
     3001 ctcgcaaacg atgccgctca tgaaggcagg ttctcaggta actgctgaac cgctcgcgct
     3061 tgaagacggc tcgtttgccg catcggcagg accgctcggg gttgtgtacg acaccaccgt
     3121 cggaggcact atttctcggt acacggtccg ctcgggggat actattgaaa ctatcgctga
     3181 aacatttggc gtttcaaaaa atactattgt ctgggcgaat gatttaaagc gcccgcctgc
     3241 tcccggaggg cgggcaggcg gtgcaacaat ttcagttggg caagagttgg ttattctgcc
     3301 ggtatcgggc gtgcggtatg tagtgcagaa atcaggagaa cgcatcgccg acattgccaa
     3361 aaaatttggc ggcgatgcgg cagaaattga gcagtataac gatgtagaca gcacagtgct
     3421 cgccaaagga accgaactta ttattcccga tggtgaaatt gctgctcctg aaccaaaaaa
     3481 atcagcaccg aaaaaagtag tacagaaaga aacatcaact ctctttgaag aagaccacga
     3541 tgatcatgaa gatgaaacgg tttccgcaaa accaaaaaaa cttccgactc gtgtttcagt
     3601 aacagtattg aaagatccaa cgggatactt tatggttcct ctaaaaacag gaacacgaac
     3661 gcagaagtta cacgggttga atggcgttga cctcgctgcg ccgactggta ccccgattta
     3721 cgccgcagca gcggggacgg ttattttagt aaagggcggc aatgcctgga atggcggcta
     3781 cggaaactat gtcgttatcg aacacggtaa tggcacgcag acgctctacg cgcacaactc
     3841 aaaaaatttg gtgagtacgg ggcaaacagt agcaaaaggt gaacaaattg cactcttagg
     3901 aagtaccgga aagtcaaccg gcccgcacgt gcactttgaa gtccgcggaa aaggcgcaaa
     3961 gaatccgctt gcaaattaaa ttttgcactt aaacactcca aaaagatttc cccaggaggg
     4021 gcttgtacaa cactttgagt ttcctataat taagtacaaa taccttgtta tgtacaatcg
     4081 tgccagttta tcacccggaa gtcgtgtcga acgaaaaggg cgcacctttt acttcgtcga
     4141 acaaaggaaa aaaagaaatg gcactatctc ctatgtattc aaaacagagg atgatgtcat
     4201 ggcttatttt ctcgaggaac caaaacaact gcacgaaaaa gagtacagta caagactgct
     4261 tttgtttatc attgctgtat gggcaatgat aagaccacta ttctaaaaag tagaaaaaac
     4321 ctgatttcat agcgaaccaa agaacctctc cggaggtttc ttttattttt accactcaaa
     4381 cttgggtctg tattggagtg cttcaagcag gtgtgcttcg gttactgctg cagcatgctc
     4441 taggtcggcg atggtgcgtg caacttttat cacgcgcagg tatgaacggg gcgaaagttc
     4501 aagccgcttg gcgtagcttt caagtagtgt ttgcagctcg ggtggcagcg caacgagctg
     4561 ctgaatatct ttggtttgca tatcggcgtt cagtcgctct ttactgaacc gttcatgctg
     4621 ctgtgcccga gcagtgctca cctgctcgcg gagtgccggg ctctcttgtg tttctttccg
     4681 ttcatggagc gcgctgtact cgatgtgtcc gacgggaacc cacaaatcaa tgcgatccat
     4741 aattggtccc gagagctttt tcttgtagcg aacaaggtcg tgcgcgctgc aggtgcaagc
     4801 gcgggttgct gagccgcggt agccgcaggg gcaggggttg agcgcagcaa cgagcaaaaa
     4861 tcgcgaaggg tactttgcgc tccccttcgc tcgggcaata gtaacggcgc ggtcttcaag
     4921 cggttcgcgg agtgcttcaa tcactcggcg atcaaattca gggaattcgt cgagatacaa
     4981 aatgccgttg tgggcaagcg ttacttcccc gggtcgcagt tgtgcaccgc caccaaccac
     5041 tgcaacgtgt gaagcggtgt ggtgcgggct gcgaaacggc acatgtgttt tccaaagttc
     5101 atctaagagt cccacactcg agtagatcat ggcagtttct aaaaactctg cttctgaaag
     5161 cggcggcaaa attcccgaga aactccgagc gagcatcgtt ttaccggtac cgggcggacc
     5221 gtagagtgca atattgtgtc cgcccgcagc ggcaattaaa agtgcgcgct ttgcttgtaa
     5281 ctgtccggac acttcaccaa aatcaaaccg tgcggtaatc gggcgctcgg taatcggctg
     5341 cggcacggtt ggggtgatga gtacttcgct ttttaggtgc gcaataagtt gtgccaatgt
     5401 ttctaccggg tagatagtga tgccggaaac gagtgcgcct tcagcactgt ttgcagccgg
     5461 aataaaaagc tcggtgcagc ctgcttttcg agcagcttca gcaatcggca aaataccgcg
     5521 cacaggtcgt acttccccat ttaaagcaag ttcgccgaca aaaatttttc ctgctgggtc
     5581 aaacgcaata tcacctgccg aaagcagata gccgagcgcg atacttacat caaagtacgc
     5641 tccttctttt ttaagttcag ccggcgagag cgagacaaca attttctgat tccgcgtttt
     5701 tggactcacg tctacgctgt gcttgagcgc cgaaagtact cggtcgcgcg cctcttcaac
     5761 cgctttgtcg ggcaagccaa caatagaaaa taaaaacagc ccccgcgaga ggtctgtttc
     5821 cactgtaaca atactcggct gcagtccgct tacctgtgcg ctctgaaggc gagaaaaatt
     5881 cattgcctca gtatagcaag aggagcgcta atcattcatg tgcgctttgc tggtgcgggc
     5941 ggcggggttt gcttcgaggc gatcagcttc aatcggctca cccgatactt cgca
//
LOCUS       DATIBQ010000017         3122 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U16294
            k141_2283681, whole genome shotgun sequence.
ACCESSION   DATIBQ010000017 DATIBQ010000000
VERSION     DATIBQ010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935789
            Sequence Read Archive: SRR25158331
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3122)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:58:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 637
            CDSs (total)                      :: 603
            Genes (coding)                    :: 601
            CDSs (with protein)               :: 601
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3122
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2283681"
                     /isolate="SMAG_U16294"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..945
                     /locus_tag="VLB02_00505"
     CDS             <1..945
                     /locus_tag="VLB02_00505"
                     /inference="COORDINATES: protein motif:HMM:NF014674.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HSE56557.1"
                     /translation="KQAGDFARELQLLLRTLGVSEANMEKGEMRVEANISLSNNEHSF
                     GTKVEVKNLNSFRSAERAIEYELARMQALYEAGRESEIIQETRGWDETKQSTFSQRSK
                     ESAHDYRYFPDPDLPKLMLAEVFDTEKMKTELPELPLQKRERYAADFGIKAEDIEVFV
                     QDAALADFYEKTVLVVGTDQTKTIANYITSDIIGNLKTVPGVQLPEQEMFAKLMGMVA
                     RSELTSRGAKDILVMLMKEGGDPQAIAKEHNMLQEHNEDALQPVIEEVVKNNEKIVAD
                     FKGGKEAALQALVGMVMKATKGSANPAVTAKLLRERIG"
     gene            962..1357
                     /locus_tag="VLB02_00510"
     CDS             962..1357
                     /locus_tag="VLB02_00510"
                     /inference="COORDINATES: protein motif:HMM:NF015658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HSE56558.1"
                     /translation="MTKNSIGFLFLLAGLVLGYVLYPTFHKKSTPVEVRQMPNDEIEQ
                     NTQMSMRQMTDGMEKLLVGKKDATFDEAFLQAMITHHQGAIAMARQVLVSTRRPELLK
                     LAQEIITAQEREVQQMQEWQAKWFGVHPY"
     gene            1475..2509
                     /locus_tag="VLB02_00515"
     CDS             1475..2509
                     /locus_tag="VLB02_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde 3-phosphate dehydrogenase
                     NAD-binding domain-containing protein"
                     /protein_id="HSE56559.1"
                     /translation="MKKVRVAINGFGRIGRAFFRVAFERGDIEIVAINDLGDLANLAY
                     LLKYDSVYRTYNHDVSHNEKGLLVDGKHVASFAEKEPSALPWGELNIDVVVESTGFFT
                     SYEGASAHIKAGAKKAVITAPAKGEPLADIASGTVLMGINEEALNTCIITSNASCTTN
                     AASPLIAILDETIGIEKAILNTVHGYTATQALVDSPAGKKGFREGRAAAANIVPSSTG
                     AAIAVTRAFPRLEGLFDGVAMRVPVIAGSIVDVTFIAKRDTSIEEVNQALKQAAGEAR
                     WQKVFSVTEDELVSSDILGSRYASVADLQFTRVVGGNLVKVLAWYDNEMGYTHALIEH
                     VVTAGNNLHE"
     gene            2694..>3122
                     /locus_tag="VLB02_00520"
     CDS             2694..>3122
                     /locus_tag="VLB02_00520"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HSE56560.1"
                     /translation="MKYIFIALIAIAIGFGAAKLATKLSTPPLPVTENQNQTPATTPA
                     AAELAPEMVNEEQQLSVVAPGGGQIDNAQVAVSFTGKKGTATYDGSFSNISSNLKLLG
                     GVVAGTVTVDTLSLTTAAPAATTNWKSAANFNAATYPIASF"
BASE COUNT          917 a          617 c          815 g          773 t
ORIGIN      
        1 aagcaagcgg gggattttgc ccgcgagctg cagctgttgc tccgcaccct gggtgtgtcg
       61 gaggcaaata tggaaaaagg cgaaatgcgc gtagaggcga atatctcgct ctcaaacaac
      121 gagcacagtt ttggtactaa ggttgaggta aagaatttaa actcatttcg cagcgctgag
      181 cgcgcgattg agtacgaact tgcccgaatg caggcgctct acgaagccgg aagagaaagc
      241 gaaataatcc aagaaactcg cggctgggat gaaacaaaac agagcacttt ctcgcagcgc
      301 tcaaaagaga gtgcccacga ctaccgctac tttcctgacc ctgatttacc aaaactaatg
      361 ctcgctgaag tatttgatac tgaaaaaatg aaaactgaat tgcctgaact gccactgcaa
      421 aagcgtgagc ggtacgcagc tgattttgga attaaagccg aggatattga ggtatttgtg
      481 caggatgcag cgcttgctga tttttatgaa aaaactgtgc ttgtggttgg aacggatcaa
      541 acaaaaacaa ttgcaaacta tattacctct gacattatag ggaatttaaa aacagtgcct
      601 ggtgtgcaat tacccgagca agaaatgttt gcgaagctta tgggtatggt tgcgcgttct
      661 gaacttacct cgcgcggagc aaaagatatc ttagtaatgc ttatgaagga gggtggtgat
      721 ccacaggcga ttgcaaaaga gcacaatatg ctgcaagagc acaatgaaga tgcactacag
      781 ccagtgatag aagaggtcgt aaaaaataac gaaaaaatcg ttgctgattt taagggtggt
      841 aaggaagcgg cgcttcaggc gcttgttggg atggtgatga aggcgacgaa ggggagcgcg
      901 aaccccgctg taacagcaaa attacttcgc gaacgtattg ggtgaggtat acttatctta
      961 tatgacaaaa aacagtattg gatttttgtt tttacttgcc ggactcgtgc tcgggtatgt
     1021 gctgtacccc acatttcata aaaaaagtac accggtagaa gtgcgtcaga tgcccaatga
     1081 tgaaatagaa caaaatacgc aaatgtcgat gcggcaaatg acggacggga tggaaaaatt
     1141 attggtaggg aaaaaagatg ccacgtttga tgaagcattt ttgcaggcga tgattacgca
     1201 tcaccaggga gcaatcgcta tggctcgtca ggtgcttgtc agtacccgcc gccctgaact
     1261 cttaaagctt gcccaagaga ttattactgc ccaggagcgg gaagtgcagc agatgcagga
     1321 gtggcaggcg aaatggtttg gcgtacatcc gtattgagag gtagttgaca aaaagtatta
     1381 aatcgtatat agtcaagggg cactatatca taccaaatac cgaaggcgcc gtgtttctac
     1441 atggggtctt ttttttattt ggtatactag ggggatgaaa aaagtacgag tagcaatcaa
     1501 cgggtttggc agaattggca gagcattctt tcgggtagcg tttgagcggg gggatattga
     1561 aatagtggcc attaacgatt taggagactt ggcaaattta gcctacttgc tgaagtatga
     1621 cagtgtttac cgaacataca atcacgatgt ttcgcataat gaaaaaggcc tcttagttga
     1681 tggcaagcac gttgcttcat ttgcagaaaa agaaccgagc gcacttccgt ggggcgagct
     1741 caacattgat gttgtagttg agtcgactgg tttctttact tcgtacgaag gtgctagtgc
     1801 gcacattaaa gctggtgcaa aaaaagcggt cattactgct ccggcaaaag gcgagccact
     1861 tgccgatatt gcatcgggta cggtgctcat gggaataaat gaagaagcgc ttaacacgtg
     1921 cattattacc tcaaatgcat cgtgcaccac taatgccgcg agcccgctga ttgcaatctt
     1981 agatgaaaca attggtattg aaaaagcgat tttaaatacg gtgcacggct acacagcgac
     2041 tcaggcgctc gttgatagcc cggcaggaaa aaaagggttt cgagaggggc gtgcagcagc
     2101 ggcaaacatt gtgccgagct caacaggggc ggctattgcg gttaccagag cattccctcg
     2161 gctcgaaggg ctctttgatg gtgttgccat gcgcgtacca gtgattgctg gctcgattgt
     2221 tgatgttact ttcatcgcca aacgcgatac cagtattgaa gaggtgaatc aagcgctcaa
     2281 acaggcagca ggggaagcac gttggcagaa ggtgttttca gtaactgaag atgaacttgt
     2341 ttcaagtgat attttaggga gccgctatgc ttcagtagca gatctccaat ttactcgagt
     2401 agtgggcggt aatctggtaa aagtattggc gtggtacgat aatgaaatgg ggtacaccca
     2461 tgcgcttatt gagcatgtgg taaccgcagg aaataactta catgaatagc atggcttgga
     2521 ttatcgtagt cttagtgttg gcggtattat tctacttttt tgttcccgtt acttatccac
     2581 cgtctaacgt aggtaaggtt cctggaccag gaattaatca ccagaaaaaa gtttctctgt
     2641 acgattacaa gaaaataaaa gatatggcac aatagtaaag tatagaataa attatgaaat
     2701 acatttttat tgcactcatc gctattgcta tcgggtttgg ggcagcaaag cttgcgacaa
     2761 agttgagcac accaccgcta cctgttaccg aaaatcagaa ccagacgccg gcaacaactc
     2821 cggcagcagc tgaacttgct cctgaaatgg tgaacgaaga acagcagtta tcagtagtag
     2881 ctccaggggg tgggcagatt gataacgctc aagtggcagt ttcttttaca ggaaagaaag
     2941 ggacggcaac ctatgatgga tcattttcaa atatatcttc aaacctaaag cttttgggtg
     3001 gtgtggtagc aggaacggtt acggttgata ctttgagtct taccaccgca gcgcctgcag
     3061 caactaccaa ctggaagtcg gcagcaaatt ttaatgcagc aacataccct attgcctcgt
     3121 tt
//
LOCUS       DATIBQ010000018         5180 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U16294
            k141_1086187, whole genome shotgun sequence.
ACCESSION   DATIBQ010000018 DATIBQ010000000
VERSION     DATIBQ010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935789
            Sequence Read Archive: SRR25158331
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 5180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:58:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 637
            CDSs (total)                      :: 603
            Genes (coding)                    :: 601
            CDSs (with protein)               :: 601
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5180
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1086187"
                     /isolate="SMAG_U16294"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..587)
                     /locus_tag="VLB02_00525"
     CDS             complement(<1..587)
                     /locus_tag="VLB02_00525"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase family protein"
                     /protein_id="HSE56561.1"
                     /translation="MSNFWQELRKNKQPFFCVAPMADVTDAAFRRLIAKYSRHGEFGG
                     GPDVFWTEFVSADGLMSQGREVLGRDLEYCEAERPIVAQLFSSNPEKMEGAARLCAEL
                     GFDGIDINMGCPDKTIEKQGAGAAMIKNPEQAQAVIRAAKRGAGNLPISVKTRVGYNT
                     VQITEWIPLLLKENIAALTVHARTRKELSAVPAPW"
     gene            complement(580..1422)
                     /locus_tag="VLB02_00530"
     CDS             complement(580..1422)
                     /locus_tag="VLB02_00530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56562.1"
                     /translation="MYTTAYQEQLLREKQPAPKKKRWKKRKLFFILLVIALGIAGIIV
                     LIRASFLQVRSVVVEGVNAADPGDVQARVEESLGGNYVFVIPKASIFFLSKDALVEML
                     TTEFPRFASVSIERRDFHTIAVRVHEYEEWALWCRSKAADDDCYFMDAAGTVFSPAPF
                     FSGTAYLKVYKGELGEMPFEPLTPVELALLKRYKEELPSLRLVPTSVSFDSPYKLTVE
                     FLYGDSTAQLFINPKHDPALVLENLSSALTSEEFKSAFRSAAKPLEYIDARFTNKVVY
                     KFNE"
     gene            complement(1427..2695)
                     /gene="serS"
                     /locus_tag="VLB02_00535"
     CDS             complement(1427..2695)
                     /gene="serS"
                     /locus_tag="VLB02_00535"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HSE56563.1"
                     /translation="MLDIKFIKENPDLIREAARKKHVQFNVDDLLKVEGERAEALNAF
                     ETARAEQNRISDQVAKETDPAKRGIMIAELKPLKEEITKKEEVLREVMKTWRELMLQV
                     PNVPDISVPEGESDAENAEVRVWGERPQFSFEPKDHSTLMEERDLVDFVRGTKVAGFR
                     GYFLKGDAMLLQFALWNYFLSKIITKGFVPLGVPSLVAKSTLYGTGYLPQGEEDLYKT
                     QDDEYLAGTGEVAAMSYYADEVLQKNQFPIKFVAFSPCFRREAGAHGKDVKGLVRVHE
                     FFKLEQIVLCEAHHEQSVSFHEEITNNVEEILQELGLHYHVVANCGGDLGLGQVKKYD
                     IEVWMPSQQKFRETHSASYFHDFQTRRLNIRYRDDDGKLKYVHSLNNTLVATPSRMIA
                     AIIENYQQVDGSIAVPEVLKPYIGKDFLGK"
     gene            2721..3587
                     /gene="dprA"
                     /locus_tag="VLB02_00540"
     CDS             2721..3587
                     /gene="dprA"
                     /locus_tag="VLB02_00540"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HSE56564.1"
                     /translation="MKSIDADFPIESLPLEQLPQLKEIPEPPKQLFIRGTLPREVKFL
                     IVVGSRLHTTYGKAACEHLIEGLRGYPVCIVSGLALGIDTIAHQAALKAQLPTIAFPG
                     SGLDWNVLYPRTNHAAAKAILEAGGALISEFNHNFRATTFAFPKRNRLVAGIADAVLI
                     IEANEKSGTLITARLATEYNRNVLAVPGPIFSNQSKGTNWLIARGAVPALSAAAILQE
                     LGLNEKPTETLRLDITEHERILLEALGSPLTRDELPDLVPLSAQEINIALTMLEIKGL
                     ITESLGKLQRIA"
     gene            3679..4110
                     /locus_tag="VLB02_00545"
     CDS             3679..4110
                     /locus_tag="VLB02_00545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56565.1"
                     /translation="MLISRIPKLSDNAKIFFKEQNINNFLELAMLPRAKTKGIFEEKL
                     RREIQNFHNDVLEYVWREEITITFSKCIPPPTRTPSRDQEKTLIALLLFEHFFGNKRQ
                     IKAVRDLYLPKLYASTNDRVHPRRSHRKTKFLAIQYFSIAS"
     gene            4176..>5180
                     /locus_tag="VLB02_00550"
     CDS             4176..>5180
                     /locus_tag="VLB02_00550"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HSE56566.1"
                     /translation="MVLLPSMKLLIVESPSKAKTIEKYLGGDYVVRASIGHIRDLPKS
                     NKKAIDIAAGFVPTYEISKGKEKVIDELRHEAEKAKEIILAPDPDREGEAIAWHLSQV
                     LHEDKKVKAPISRVTFHEITKGAIEEALKHPRKIDMNLKTAQEARRVLDRLFGYDLSG
                     LIWKKVRYGLSAGRVQSPALRILMEREREIRAFIPETFWVISGLFETQKKEQLSLTCA
                     EEPRDRAVVEKILSEGKSGTWNVLDVKETEAKRSPRAPFTTSTLQQTASSRLGFSPKR
                     TMMTAQKLYEAGHITYMRTDSTTLAKTAQEAIADVIEKLHGKELVEIRSYQTKSKNAQ
                     E"
BASE COUNT         1438 a         1324 c         1079 g         1339 t
ORIGIN      
        1 tcccacggag ccggtactgc cgagagttct ttgcgggtgc gtgcatgtac cgtcagtgct
       61 gcaatgtttt ctttcagaag gagtgggatc cattcagtaa tttgcaccgt gttgtagcca
      121 acgcgagttt ttacggaaat gggcaggttg cccgcgccgc gtttcgcggc gcggatcacc
      181 gcttgggctt gttctggatt ttttatcatt gccgccccag cgccttgctt ttcaatcgtt
      241 ttatcggggc agcccatgtt tatatcgata ccgtcaaacc caagctcggc acagagtcgc
      301 gctgcacctt ccatcttttc aggatttgaa gaaaagagct gggcaacaat tggtcgctct
      361 gcttcacaat attccaaatc gcgcccgagc acttctcgcc cctgactcat caaaccatca
      421 gcggaaacaa actcagtcca aaatacatcg ggtccgcctc caaattctcc gtgtcgtgag
      481 tactttgcga tcaacctacg gaatgctgcg tcggtcacat ctgccattgg cgcgacgcag
      541 aaaaagggct gcttgttttt tcggagctcc tgccaaaaat tactcattga atttgtagac
      601 taccttgttg gtgaatcgcg cgtcaatata ctcgagcggt tttgccgcac ttcgaaatgc
      661 tgatttaaat tcttctgaag tgagtgctga agaaaggttt tcgagcacca gcgccggatc
      721 gtgttttgga tttataaaaa gttgtgcagt actatcgcca tacaaaaact ctaccgtaag
      781 cttgtatgga gaatcaaacg agacgctcgt tggcaccagc cgcagactag gaagctcttc
      841 cttgtagcgc ttaagaagcg cgagctctac tggggtaagt ggttcaaacg gcatttcacc
      901 aagctcccct ttgtatactt ttaagtatgc cgtacccgaa aaaaatggag ctggactaaa
      961 aacagtacct gctgcatcca tgaagtaaca gtcgtcgtct gctgctttac tgcggcacca
     1021 gagcgcccac tcctcatact cgtgcacacg cactgcaata gtatgaaaat ctctccgctc
     1081 tatagaaact gaagcgaagc gcggaaattc ggtagtgagc atttctacca atgcatcttt
     1141 gctcaaaaaa aatatactcg cttttggaat aacaaacaca tagtttcccc cgagcgactc
     1201 ctctacccgc gcctgcacgt cgcccggatc ggcagcgttt actccttcaa ctacaacact
     1261 acgcacctgt aaaaacgacg cgcgaataag aactataatc ccagcaatac cgagcgcaat
     1321 aaccagcaag ataaaaaata attttctctt tttccagcgc ttcttttttg gtgccggctg
     1381 cttctcgcgc agcagctgct cctggtacgc tgtggtatac attggcttac ttacctaaaa
     1441 aatcttttcc aatatagggc ttgagcactt ccggcaccgc aatactacca tcgacttgtt
     1501 ggtaattttc aataatcgca gcaatcatcc ggctcggtgt tgcaactagt gtgttattga
     1561 gtgagtgcac atactttagt tttccgtcat catcgcggta gcgaatgttt aaacgtcgag
     1621 tttgaaaatc atgaaaatat gatgctgaat gcgtttcgcg aaacttttgc tgagagggca
     1681 tccatacctc aatatcgtac ttttttactt gccccaaccc taaatcacca ccacaatttg
     1741 ccactacatg gtagtggagt ccaagctctt ggagtatctc ttcaacattg tttgtaatct
     1801 cctcatgaaa ggaaacactc tgttcgtgat gcgcctcaca gagcacaatc tgttcaagct
     1861 taaaaaactc gtgtacccga acaagccctt ttacatcttt gccgtgtgca cctgcttctc
     1921 ggcgaaagca gggcgagaat gcgacaaatt taatggggaa ttgatttttc tgaagtactt
     1981 catcagcgta gtacgacatc gctgcaacct ccccggtacc tgccagatat tcatcatctt
     2041 gagttttata taaatcttct tccccttgtg gcaagtagcc ggtgccgtag agcgtactct
     2101 tcgccaccaa tgaaggcacc ccaagtggca caaacccttt cgtgattatt ttacttaaaa
     2161 aataattcca gagtgcaaat tgtagtagca ttgcatcgcc ttttaaaaag tatccacgga
     2221 agccagccac ttttgtaccg cgaacaaaat caaccaaatc gcgctcttcc ataagagtac
     2281 tatgatcttt tggctcaaat gaaaattgtg gtcgctcacc ccagacacgc acctcagcat
     2341 tttctgcatc actctctcct tcaggtacac ttatatcggg aacgttcggc acctgaagca
     2401 tcagctcgcg ccaggttttc attacttcac ggagtacctc ttcttttttt gttatctctt
     2461 ctttaagcgg tttaagctcc gcgatcataa tcccacgctt tgcggggtcg gtctctttgg
     2521 caacctggtc agaaatgcgg ttctgctctg cccgagcagt ttcaaaagca ttcagtgctt
     2581 ctgcacgctc tccctcaacc ttcaagaggt catcgacatt aaattgaaca tgtttctttc
     2641 gtgccgcctc gcggattaaa tcggggtttt ctttgatgaa tttaatatcg agcatgggta
     2701 aactataata aactatagcc atgaaatcaa tcgatgccga ttttcctatt gagtcactgc
     2761 cacttgagca attgccgcaa ctgaaagaaa tcccggaacc gccaaagcaa ctctttatcc
     2821 gcggcaccct gccacgtgag gtaaaatttt taattgttgt tggctcacgc ctccacacca
     2881 cctacggcaa agctgcctgc gagcacctta ttgaaggctt gcgtggctac cccgtgtgta
     2941 ttgtttcagg actcgcactc ggtattgata ccattgcaca ccaagcagca ctaaaggcac
     3001 aactgcccac catcgccttc cccggatcag gactcgattg gaacgtcctc tacccccgaa
     3061 ccaaccatgc ggctgcaaaa gcaatccttg aagcaggcgg tgcactcatt tcagaattta
     3121 atcacaactt tcgtgccacc acctttgcat tcccaaaacg taatcggctc gttgccggta
     3181 tcgccgatgc agtacttatc attgaagcga atgaaaaatc aggcaccctt attactgcac
     3241 gactcgccac tgaatacaac cgcaacgtgc tcgcggttcc tggaccaatt ttttctaatc
     3301 aatcaaaagg tactaactgg ctcattgccc gcggtgctgt ccccgcactt tctgccgcag
     3361 ctattttgca agagctcggt ttgaatgaaa aaccaaccga aaccctccgg ctggatataa
     3421 ctgaacatga gcgcattctc ttagaagcgc tcggtagccc actgacccga gatgaactcc
     3481 ctgatctcgt accactctct gcacaagaga taaacatcgc tctgaccatg cttgaaataa
     3541 aaggactcat taccgagagt ttagggaaat tacaacgcat tgcctaagga gagaaagatg
     3601 ggtatagtat aaaaaaatat ctctcttatg atgattttta aaaccgaaaa acaagtgcct
     3661 gtgttgtttg ctaatcgtat gctcatcagt agaattccta agctctcaga caacgcaaaa
     3721 atttttttta aggagcaaaa catcaacaat tttcttgaac ttgcgatgct ccctcgagcg
     3781 aaaactaaag gtatcttcga agagaaactt cgaagagaaa ttcaaaactt tcacaatgat
     3841 gtgctggagt acgtatggag agaagaaata actattacct tttcaaagtg tattcctcca
     3901 ccaacgagaa ccccatcgcg agaccaagaa aaaacattaa ttgccttatt actgtttgag
     3961 catttttttg gaaacaaacg gcagataaaa gcagtaaggg acttgtacct tcctaaattg
     4021 tacgcaagta ctaatgaccg tgttcacccg cgaagatctc accggaaaac aaaatttctt
     4081 gcaatacagt atttcagtat tgcttcataa ccatacaaag cccgtctcct tgaggcgggt
     4141 ttcttttttt ataccattgc taaaaaaatg tgtgcgtggt attacttcct tccatgaagc
     4201 tcctcattgt cgaatcgccc tcgaaagcaa aaacaattga aaagtattta ggtggcgact
     4261 atgtcgtacg tgcctcaatc gggcacattc gcgacctgcc aaaatcaaat aaaaaagcga
     4321 tcgacatcgc tgctggtttt gtgcccacct acgaaataag taaagggaaa gaaaaagtga
     4381 ttgatgaact gcgccacgag gcggagaaag cgaaagaaat tatactggcg cccgaccctg
     4441 accgcgaagg tgaagcaatt gcctggcacc tttctcaggt gctccacgag gataaaaaag
     4501 taaaagcacc catctcgcgt gttaccttcc acgaaattac caaaggagca atagaagaag
     4561 cactcaaaca ccctcgaaag attgatatga acctgaaaac tgctcaagaa gcgcgccgtg
     4621 tgcttgaccg tcttttcggc tacgatctct cggggcttat ttggaagaag gtgcgctacg
     4681 gactttctgc ggggcgagta caatcaccgg cgctgcgcat tttaatggag cgcgagcgcg
     4741 aaattcgcgc tttcattcct gaaacattct gggtcatcag cggacttttt gaaacacaaa
     4801 aaaaagagca actcagcctt acctgtgctg aagaaccgcg ggaccgtgca gtggtggaaa
     4861 aaattctctc agaaggaaaa agtggcacct ggaacgtgct ggatgtaaaa gaaaccgaag
     4921 caaagcgctc tcctcgtgca cccttcacta cctcgacctt gcagcaaact gccagctctc
     4981 gcctgggttt ctcgccaaag cgcacgatga tgactgccca aaaattatac gaagcgggac
     5041 acattaccta catgcgcacc gattccacta ccctcgcaaa aactgctcaa gaagcaattg
     5101 ctgacgttat tgaaaaactt catggaaaag aattggtgga aataagaagc taccaaacaa
     5161 aatcaaaaaa cgctcaagaa
//
LOCUS       DATIBQ010000019         3696 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U16294
            k141_1078766, whole genome shotgun sequence.
ACCESSION   DATIBQ010000019 DATIBQ010000000
VERSION     DATIBQ010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935789
            Sequence Read Archive: SRR25158331
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:58:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 637
            CDSs (total)                      :: 603
            Genes (coding)                    :: 601
            CDSs (with protein)               :: 601
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3696
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1078766"
                     /isolate="SMAG_U16294"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..342
                     /locus_tag="VLB02_00555"
     CDS             <1..342
                     /locus_tag="VLB02_00555"
                     /inference="COORDINATES: protein motif:HMM:NF015664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HSE56567.1"
                     /translation="AKALKTARKNAIKIKFTEKRSIPHEVHAKYNSSRVTIFPNHGRG
                     LVSGAAIRSILDIAGVKDVSSKVLSGSKNKLNTARATIKALSLVASKFAPAAKIIEEP
                     IVVPEIPVVEK"
     gene            350..787
                     /gene="rplO"
                     /locus_tag="VLB02_00560"
     CDS             350..787
                     /gene="rplO"
                     /locus_tag="VLB02_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HSE56568.1"
                     /translation="MLHTLKSHTKNKKPKLVGRGGKRGKTAGRGTKGQKARAGHKIRP
                     HIRDVIKKLPKLRGRGKNINKAFQLKPAVVNLSVLEALFETGAMIRPQVLLERGLIKR
                     QQGTIPAVKILATGELTKKLSFFDCSVSAAAKAKIEAVGGTVK"
     gene            798..2075
                     /gene="secY"
                     /locus_tag="VLB02_00565"
     CDS             798..2075
                     /gene="secY"
                     /locus_tag="VLB02_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HSE56569.1"
                     /translation="MQTVLSKLKILFTERQLRNRLLFVLAALFMFRLLASIPLPGIDE
                     TKLNEFLFGNQLVGLFNLFSGGGLSNLSIVLLGVGPYITASIIMQLLTMMFPNLKELY
                     HESGEMGRKKFNNYTRLLTIPLAALQAFSLLVLFKRQGVFPDLTSFDMAVNICIAIAG
                     SMLLMWIGELVTQFGIGNGVSLVIFAGIVAALPSSVSQFFFTFTASQLPTLILFVAVG
                     LLVIAGVILVTEAERPIPVTYARQVRVAGLTTKNANGTYLPLRLNHAGVIPIIFALSI
                     LLFPQLIANLLQASANGTLQSIASGITAFLQNTWAYAATYFVLVVLFTYFYTAVTFDP
                     KQIATNLQKSGAFVPGIRPGESTTQYLGSIVSRITIVGALFLGLIAVLPLIARAVTGI
                     SALTLGGTALLIVVSVIIDISRKLDAQIAMHEY"
     gene            2106..2702
                     /locus_tag="VLB02_00570"
     CDS             2106..2702
                     /locus_tag="VLB02_00570"
                     /inference="COORDINATES: protein motif:HMM:NF012624.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside monophosphate kinase"
                     /protein_id="HSE56570.1"
                     /translation="MFEQPLVTFFIGQSGCGKGTQADLLENRLKELDPPRKMFRLETG
                     DTFREFITGSSYTARLTKQLMDHGKLVPPFLAVHVWSHLLIEGYQGDQHVLFDGTPRV
                     FAEVAALDSAIHFYNWRPHVIFLNVSDGWARKRLGERQRADDQEASDVEERLHWFHTS
                     VIPVIQYLQTTPPYIFHEINGEQAIEQVHADIKKALNI"
     gene            2736..3503
                     /gene="map"
                     /locus_tag="VLB02_00575"
     CDS             2736..3503
                     /gene="map"
                     /locus_tag="VLB02_00575"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HSE56571.1"
                     /translation="MVIIKTKDEIVRLKEGGPLLARILATVAAAAKPGVTTASLDALA
                     ATLIQEAGGKAAFLNYQPEGADYPYPASLCVSINSEVVHGIPGTRILKEGDIVSLDLG
                     LNHKGVFLDHAVTVAVGAAHPKDKTLLAVCHEALMAGIAAARGGNTVGDIGYAIQSVI
                     KPYKFGIVRTLSGHGVGRAIHEDPYVPNYGKPGKGEKLKPGMVIAIEPMITRGSEEVY
                     TAADGYTIKTADNSRAAHFEHTILITEGEPEILTIEK"
     gene            complement(3539..3694)
                     /locus_tag="VLB02_00580"
     CDS             complement(3539..3694)
                     /locus_tag="VLB02_00580"
                     /inference="COORDINATES: protein motif:HMM:NF012667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HSE56572.1"
                     /translation="MNAAVRELGGTYSTFAGALKKANIEVDRKILATLAQKNPESFKR
                     IYEKATK"
BASE COUNT          913 a          928 c          886 g          969 t
ORIGIN      
        1 gctaaagcac ttaaaactgc ccgtaaaaat gcaatcaaga ttaagttcac tgaaaagcgt
       61 tcgattccac acgaagtgca cgcaaagtac aacagctcgc gcgttactat tttccctaac
      121 cacggccgcg ggctggtgtc aggcgcagca atacgatcaa ttcttgatat tgcgggggta
      181 aaagatgtaa gctcaaaagt gctctcaggc agcaagaata agctcaacac cgctcgggct
      241 accatcaagg cgctctcgct tgttgcatca aagtttgcgc cggcagcaaa aattattgaa
      301 gagccgattg tggtgccaga aattccggta gtagaaaaat aatacttgta tgttgcacac
      361 actcaaatca cacacaaaaa ataaaaagcc aaagcttgtt ggtcgcggtg gcaagcgcgg
      421 taaaactgct ggtcgcggta ccaaggggca gaaggcgcgt gccgggcaca aaattcgccc
      481 gcacattcgc gatgtcatta aaaagctccc gaaacttcgc ggtcgcggaa aaaacatcaa
      541 caaggcattc caactcaagc cagcagtagt aaatctttca gtacttgaag cactctttga
      601 aactggtgca atgattcgcc cgcaggtact ccttgagcga gggcttatca agcgccagca
      661 gggaaccatc ccagcggtaa aaattttggc aacgggtgaa ctcaccaaaa aactctcgtt
      721 tttcgactgc agtgtttctg ctgctgcgaa agcaaaaatt gaggctgtgg gaggaaccgt
      781 aaaataattt cgtactcatg caaacagtat tgtcaaagct aaaaatcctt ttcactgaaa
      841 ggcagttgcg taaccgcctc ctttttgtgc ttgcggcact ttttatgttt cgcctcttgg
      901 catctattcc acttccggga attgatgaaa caaagttaaa cgaatttctc ttcggcaacc
      961 agctggtggg gctctttaac ctcttctcag gtggtggact ctcaaacctt tctattgtgc
     1021 tcctcggtgt tggtccatat atcactgcct cgattattat gcaattgttg acgatgatgt
     1081 tcccaaactt gaaagagctc taccacgaga gtggagagat gggacgaaag aagtttaata
     1141 actatacgcg gcttctcact attccgcttg cagcactcca ggcattctcg ctcttagtgt
     1201 tgtttaagcg tcagggagta tttcccgacc ttacctcgtt cgacatggcg gtcaatatct
     1261 gtatcgctat tgccggctca atgctcctga tgtggattgg tgagctcgtt acccagtttg
     1321 gtatcggtaa tggtgtttca ctcgttattt ttgccggcat tgtggcagcg ctgccgtcga
     1381 gtgtttctca atttttcttt accttcaccg caagccagct gccaacactt atcttgtttg
     1441 tggcagtagg gctcttggtt atcgctggag ttattttagt cactgaagcc gagcgcccaa
     1501 ttccagtaac ctacgcccgc caggtgcgcg ttgcagggct aacaactaag aatgcaaacg
     1561 gtacgtacct gccgctgcgc ttaaaccacg ccggcgtgat cccaatcatt tttgctctct
     1621 ctatccttct tttcccgcag cttattgcta acttgctcca agcatctgca aacggcacac
     1681 tccagagtat tgcttctggt attactgcct ttctgcaaaa cacttgggca tatgctgcta
     1741 cctactttgt actcgtggtg ctctttacct acttttacac tgcggttacc ttcgatccaa
     1801 aacaaattgc caccaatctg caaaagagcg gtgcgttcgt tccggggatt cgtccgggcg
     1861 aatcaacgac gcagtacctc ggatcaattg tttctcggat tacgattgtt ggcgcactat
     1921 ttcttggctt aatcgccgtg ctcccgctta tcgctcgtgc agtcaccggg atttcagccc
     1981 tcacgcttgg cggcactgca ctcctcatcg ttgtttcagt gatcattgat atctctcgca
     2041 aactggatgc acagattgcc atgcacgagt attaagagct cccgtgttag tgctatactt
     2101 tttatatgtt tgaacaaccg ctcgtcacct tctttatcgg tcaatcaggc tgtgggaagg
     2161 ggacccaggc tgatttactg gaaaaccggc tcaaagagct cgaccctcct cgcaagatgt
     2221 tccgccttga aacgggtgat acctttcgtg agtttattac gggcagctcc tatactgctc
     2281 ggcttaccaa gcagctgatg gatcacggca agctcgtacc accatttttg gcagtgcatg
     2341 tgtggtcaca tttgcttatt gaaggctacc agggcgacca gcacgtactg ttcgacggta
     2401 caccgcgagt gtttgctgaa gttgccgcac ttgattctgc tattcatttt tacaactggc
     2461 gcccgcacgt tatcttttta aatgtttctg acggctgggc gcgtaagcgg ctgggcgagc
     2521 gtcagcgagc agacgaccaa gaagcatccg atgttgaaga gcgcctgcac tggttccaca
     2581 cctcggttat tccggtgatt cagtatttac aaacaacccc gccctacatc ttccacgaaa
     2641 taaacggcga gcaggcaatt gagcaggtgc atgccgatat caaaaaagca ctcaacatat
     2701 gattattgga atgtggtgat tggttattaa ttcctatggt tattattaaa acaaaagatg
     2761 aaatagtgcg ccttaaagaa ggtggaccac tactggctcg catcctcgcc accgttgccg
     2821 ctgcagcaaa accaggagtt accacagcct ctttagatgc gttggcagct acgcttatac
     2881 aagaagccgg aggcaaagca gcctttctta actaccagcc tgaaggtgcc gattacccgt
     2941 atccggcaag cctctgtgtt tcaataaaca gcgaagtggt gcacggtatt ccgggaactc
     3001 gaattttaaa agagggcgat attgtgtcgc tcgacctcgg gctcaaccat aagggagtgt
     3061 ttctcgacca cgcggttacc gttgcggtgg gtgctgcaca tccaaaagat aaaacactgc
     3121 ttgccgtgtg ccacgaggcg ctgatggcag gcattgcggc cgcacgtgga ggcaacacag
     3181 tgggagacat tggctacgct attcagtcag taattaagcc gtacaagttt ggcattgtcc
     3241 gcacgctctc ggggcatggg gtaggtcggg caatccacga agacccgtac gtgcccaact
     3301 atggcaagcc gggaaaaggg gaaaaattga agccggggat ggtcattgct attgagccca
     3361 tgattactcg tggcagcgag gaagtctaca ctgcagcgga cggctatacc ataaaaaccg
     3421 ccgacaactc ccgcgccgcc cactttgagc acactatttt gatcactgaa ggggagcctg
     3481 aaattttaac catagaaaaa taaaaattcc ctcgaattga gggaattttt tagtcaattt
     3541 attttgtagc cttttcgtaa atccgcttga agctttcagg attcttctgg gcgagagtgg
     3601 cgaggatttt gcggtcaact tcgatgttgg ctttcttcag tgccccagca aacgtgctgt
     3661 aggtgccgcc gagctcacgt actgctgcgt taattt
//
LOCUS       DATIBQ010000020         5708 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U16294
            k141_1590076, whole genome shotgun sequence.
ACCESSION   DATIBQ010000020 DATIBQ010000000
VERSION     DATIBQ010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935789
            Sequence Read Archive: SRR25158331
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 5708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5708)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:58:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 637
            CDSs (total)                      :: 603
            Genes (coding)                    :: 601
            CDSs (with protein)               :: 601
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5708
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1590076"
                     /isolate="SMAG_U16294"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1321
                     /locus_tag="VLB02_00585"
     CDS             <1..1321
                     /locus_tag="VLB02_00585"
                     /inference="COORDINATES: protein motif:HMM:NF012239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HSE56573.1"
                     /translation="TKAAITFLDTPGHAAFTGMRERGALTADIAVLVVSAEDGVKAQT
                     LEALKTIQTAKIPFVVAINKIDRPEANIEKTKMSLAEHEIFIEGYGGTIPVVPISALT
                     GAGIDDLLETLLLVAELEELTGDPSAPAEGIVIESRLDPKRGISGTIVITNGTLKKGM
                     FVVAGDAYASTRIIENFLGKSIESATFSSPVQLVGFNTSPTVGSPIAIAATKREAEAM
                     TEEYAQAKKDAKEQVVIGDGVRIVPVIIKADVYGTAEALEKELKKIQVQDVYVKVISK
                     GIGAVTESDMKHATADKDAIILAFNVAIDSKVREMSEQTGITVQSFNIIYKLTEWFAE
                     EAERRRPRKEIRDIIGTAKVLKVFSKTKERQVIGCRVEAGALAEQARVTIKRRDFELA
                     TGTIIGLEAGKQKTREVKEGSDCGVLIETKTDIAPGDVLEAFVMATR"
     gene            1327..1647
                     /locus_tag="VLB02_00590"
     CDS             1327..1647
                     /locus_tag="VLB02_00590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56574.1"
                     /translation="MERNEKIELRIKELAALFFGRESNRTALITVTRVVVADRGRSAM
                     IYVTVLPESAEAGALSFAKRKRAELREFVKEQLNIHPIPFLDVTIDSGEKARHTIDVL
                     LKEE"
     gene            1652..1723
                     /locus_tag="VLB02_00595"
     tRNA            1652..1723
                     /locus_tag="VLB02_00595"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1684..1686,aa:Cys,seq:gca)
     gene            1754..1838
                     /locus_tag="VLB02_00600"
     tRNA            1754..1838
                     /locus_tag="VLB02_00600"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1788..1790,aa:Leu,seq:taa)
     gene            1844..1915
                     /locus_tag="VLB02_00605"
     tRNA            1844..1915
                     /locus_tag="VLB02_00605"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1877..1879,aa:Arg,seq:ccg)
     gene            2184..2265
                     /locus_tag="VLB02_00610"
     tRNA            2184..2265
                     /locus_tag="VLB02_00610"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2218..2220,aa:Leu,seq:tag)
     gene            2293..2368
                     /locus_tag="VLB02_00615"
     tRNA            2293..2368
                     /locus_tag="VLB02_00615"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2326..2328,aa:Lys,seq:ctt)
     gene            2488..2724
                     /locus_tag="VLB02_00620"
     CDS             2488..2724
                     /locus_tag="VLB02_00620"
                     /inference="COORDINATES: protein motif:HMM:NF013691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HSE56575.1"
                     /translation="MYFVYILKLKNGEYYTGFTDNLKRRVQDHRYGKVITTRSQLPLQ
                     LVWYASFLSKKKALDFELYLKSSSGFAFRNKRFI"
     gene            2892..4187
                     /locus_tag="VLB02_00625"
     CDS             2892..4187
                     /locus_tag="VLB02_00625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56576.1"
                     /translation="MGILIILSIVALLIWWAATFKIYKPVSGQLSAITLFNMIIITVG
                     PGVNDRTWEIKDPSGTIIPLSLKLQWWQKLASYILFWPLGVKEFNYHYKKFRPFKDSV
                     GKDAFIEWKPILEEGEELTPETEVLIDYRESKKNSLFVFERDEFTMPFISQDAIKGVA
                     KARIEFAIYGFESAVSDLINFKEDPIVAAQDLFRNWASEKIYRTSINGVSFDVVQEEA
                     KVAGLVTGENFLQTLNYGIYENRICVVVTDIKIMRFYLEPTSLDVIEAQEAIEREKLE
                     QERRRTEAQTKKIEAEGLAAAKAILAEGEANAIRLTAAANAGKIDIEGKAENIIKISL
                     LQQQNTAAVNLLAETKKIEVKNAGKILTRLQRYKKKIAPIKDATEVAVAENLGKTKGV
                     IVLGDGLGKKGVTSDLDNLSEKIVSQIIATNVINQKQGG"
     gene            4189..4851
                     /locus_tag="VLB02_00630"
     CDS             4189..4851
                     /locus_tag="VLB02_00630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56577.1"
                     /translation="MFKKIAPSLLVIIPIAVLLVLGSFGYTLLNKLTSKWNGFPEWGN
                     VLIVFIVFLVLLWKFHSHLKKLPKSVFQTITTVVVIIGILLGIKLIWWAATSTFAESY
                     SSEETEQSAHNNQKGIGSKDRSYGMKLGEPFELEPGAEKAITLPFYADAYYSTTEKLR
                     VRNFSSTVSFTVNKNGSVTDAENVEVQKNTVQTQEAGVVWYIKNLGDKKAQVLVTSSA
                     SD"
     gene            complement(4917..5267)
                     /locus_tag="VLB02_00635"
     CDS             complement(4917..5267)
                     /locus_tag="VLB02_00635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56578.1"
                     /translation="MFETTLYAAEGIQELVFKLNKFILNPLIILAFSLALAYFLWGLV
                     AFIKNAANAEARKTGQQHMLWGIVGMVIMVSALGIINLLVNVFGIKGVDVEQQTIKTQ
                     AIPPVKIKGFNTNP"
     gene            complement(5274..5666)
                     /locus_tag="VLB02_00640"
     CDS             complement(5274..5666)
                     /locus_tag="VLB02_00640"
                     /inference="COORDINATES: protein motif:HMM:NF039493.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56579.1"
                     /translation="MKNKFFTLFLTLSSFALAAPAFAQNTGQIDFWVAKVFEYARMGV
                     TFLMIVATLYFIWTVIGFITTADAAKRADKRKQMVAGIIGLFVIVSVWGIIYFVGNTL
                     GVQRGSGSNLGLPCPPGTRAIGPAGSCI"
BASE COUNT         1786 a         1180 c         1286 g         1456 t
ORIGIN      
        1 caccaaagcg gcaattactt ttctcgacac cccgggacat gcggcattta ccggtatgcg
       61 cgagcgaggc gcactcaccg ccgatattgc agtgctggta gtttcagctg aagacggggt
      121 aaaagcacaa acattagaag cgctcaaaac tattcaaaca gccaagattc cttttgtggt
      181 cgcgatcaat aaaatcgacc gcccggaagc aaatatcgag aaaacaaaaa tgagcctcgc
      241 cgagcacgaa atatttattg aaggctacgg cggtactatt cccgttgttc ccatttcagc
      301 tcttacgggt gcagggattg atgacttgct tgaaacacta ctcttggttg cagaacttga
      361 agagctcacc ggcgacccga gcgctcccgc agaaggaatc gtcattgaat cgcgcctcga
      421 cccaaagcgc ggcatttcgg gcacgattgt aataacaaac ggaacgctga aaaaaggaat
      481 gtttgtagtt gccggcgatg cctacgcatc aacccgcatt attgaaaact ttcttggcaa
      541 gagcatcgaa agcgcaacat tttcttcgcc ggtgcagctt gttggtttta atacttcccc
      601 taccgtcggg agccctattg ccattgccgc taccaaacgt gaagctgagg caatgaccga
      661 agaatacgct caagcaaaaa aagatgccaa agaacaggta gtgattggcg acggtgtccg
      721 gattgtccct gttattatta aggcagacgt gtacggcacc gccgaagcac ttgagaaaga
      781 gttgaaaaag attcaggtgc aggatgtata tgtaaaggta attagcaagg gtatcggcgc
      841 cgttactgaa tcagatatga agcacgccac tgccgacaaa gatgcaatta ttttagcgtt
      901 taatgttgca attgacagca aagtacgcga aatgagcgag cagacaggca tcaccgtaca
      961 atcatttaat attatttata aactcaccga atggtttgct gaagaagctg agcgccgccg
     1021 cccccgcaaa gaaatacgcg acattattgg caccgcaaaa gtgttgaaag tattttcaaa
     1081 aacaaaagag cgccaggtga tcggctgccg agttgaagca ggcgcgcttg ccgagcaggc
     1141 gcgggttacc atcaagcggc gcgattttga actggcaacc ggcactatta tagggcttga
     1201 ggcaggcaag caaaaaaccc gagaagtaaa agaaggcagc gactgcggtg tgttaattga
     1261 aacaaaaacc gatattgcac cgggagacgt acttgaggca tttgtgatgg caacccggta
     1321 gcacgtatgg aacgaaacga aaaaatcgaa ctacggatta aagaactggc tgcgctcttt
     1381 tttggacgtg aatcaaatcg caccgctctt attactgtaa cccgcgtcgt agttgccgat
     1441 cgtggtcgca gcgccatgat ttacgtaacc gtactgcccg aaagcgcaga agccggagca
     1501 ctctcgtttg cgaaacgaaa gcgagctgaa ttgcgcgagt ttgtaaaaga gcaattaaac
     1561 attcacccaa tcccctttct tgatgttacc attgactcgg gcgaaaaagc ccggcacact
     1621 attgacgtgc ttttaaaaga agaataaaaa agacctggtg gccaagtggt aaggcggttc
     1681 tctgcaaaag aactatacgc gagttcgatt ctcgcccagg tctcattccc ttccctgaag
     1741 ggaaaaacag tttgcccgga tggcggaatt ggtagacgca gaggacttaa aatcctcggc
     1801 ccttatacgg cgtgtgggtt cgattcccac tctgggcaca gaagcttccg tagttgaatg
     1861 gatacaatac tcccctccga agggagtggt gcaggttcga ttcctgccgg aagcacaaaa
     1921 acttgctaaa gtttttgtgc tgaagagcaa gcaaactgct ttgcttacgt gcagaaatcg
     1981 aacgccggaa tatgtcgagc gagcacgcga aactatgagg cggtgcccag acaactcacc
     2041 tgcaggcgag ttagtcgcgg gagataagcc gaagacggtc ctgcaagaag cacactaaca
     2101 tgggtgagat agcgaagcgt cctgctcctg aaccattctc atattttaaa atcaaacaaa
     2161 atatgctata cagtaagctg cctgctgggg tggcgaaatt ggtagacgca cctgccttag
     2221 gagcaggcac cgcgaggtat ggaggttcaa gtcctctccc cagcacattg ttttatagtt
     2281 ttagaataat atgcccctat agctcagttg gtagagcagc tccctcttaa ggagacggtc
     2341 gtaggttcga atcctactgg gggcaccact tcgctcctga gggagcttcg cggtgcacag
     2401 caccaaacaa ataattttaa ttttgttact atagttaaat aacattagcg aagtggcccc
     2461 gcaaagccca agcggcgtgg ggcaattatg tattttgtct atatcctcaa attaaaaaat
     2521 ggagaatatt acacaggttt tactgataac cttaaaaggc gagtgcaaga tcatcggtat
     2581 ggaaaagtaa tcaccactcg atcacagctg ccgctccaac ttgtttggta cgcttcattt
     2641 ctatcgaaaa agaaagcact tgattttgaa ttgtatttaa aatcaagttc tgggtttgcc
     2701 tttcggaata aaagatttat ttaaattcga atttactaca acaaatttag tcgaaggaga
     2761 aactcctact gggagtacag aaaaaccctt tgattagttt ttctgtactc ggctcgtttg
     2821 acaaaatctt atttttatgt ataatgaaat tcagaaaatt taattttgta aatccttaac
     2881 aatttaatac tatgggcata cttattattc ttagtattgt tgctctcctt atttggtggg
     2941 cagcaacctt taaaatttac aagcctgtca gtggacaact ctctgctatt accctattca
     3001 acatgattat tataactgtt ggaccaggag ttaatgatcg cacctgggaa attaaagatc
     3061 cgagcggaac aataattcca ctatctttaa agcttcagtg gtggcaaaaa ttggcttcgt
     3121 atatcttgtt ctggcctctt ggagttaaag aatttaacta tcactacaag aaatttagac
     3181 cattcaaaga cagtgtagga aaagatgctt ttattgaatg gaaaccgatt cttgaagaag
     3241 gtgaagaatt aacaccagag actgaagtgc tcattgatta tagagaaagt aagaaaaatt
     3301 ctctgtttgt atttgagcgt gacgaattca ctatgccatt tatttcgcaa gatgcaatta
     3361 aaggtgttgc gaaagcgcga atagaatttg cgatatacgg tttcgaatcc gcagtaagtg
     3421 atcttattaa ctttaaagaa gatccgattg tcgccgcaca ggacttgttc cgaaattggg
     3481 catcagaaaa aatctaccgt accagcatta atggagtttc gttcgatgtc gtacaagaag
     3541 aagcaaaagt tgccgggcta gttactggtg aaaacttctt acagacactc aattatggaa
     3601 tttacgagaa tcggatctgc gtagttgtta ctgatattaa aatcatgcgg ttctaccttg
     3661 aacctacctc cctagatgta attgaagcac aagaagctat agagcgggaa aaactagaac
     3721 aggaacgcag acggactgaa gctcaaacca agaaaattga agctgaaggt cttgcagctg
     3781 caaaagccat attagctgaa ggtgaagcaa atgctatccg gttaacagca gcagcaaatg
     3841 ctggaaaaat tgacatcgag ggcaaggcgg agaacattat taagatttcc ttattgcaac
     3901 aacaaaatac tgcagcagtg aatcttttgg cagagactaa aaaaattgaa gtaaaaaacg
     3961 ccggaaaaat tctcaccaga ctacagaggt acaagaaaaa aattgctcca attaaagacg
     4021 ctaccgaagt agcagtagca gaaaaccttg gaaagacaaa aggggtaatt gttctcggtg
     4081 atgggttagg aaaaaaaggt gttactagcg accttgataa tctctctgag aagattgttt
     4141 cgcaaattat tgcaactaat gtaataaacc aaaaacaagg aggatagtat gtttaaaaaa
     4201 atcgcaccat cgctcttggt tatcattcct atagctgttt tattagtact tggttcgttt
     4261 ggctatacat tactgaataa acttacctca aaatggaatg gttttcctga atgggggaat
     4321 gtactaattg tctttattgt atttttggta cttctctgga aattccatag ccatcttaag
     4381 aagctaccaa aatcagtttt ccaaactatt accactgttg ttgtaataat tggaattctt
     4441 ttggggataa aacttatttg gtgggcagca acctcaacgt ttgctgaaag ttattcatcg
     4501 gaggaaacag agcagtcggc tcacaacaac caaaaaggaa ttggatcaaa agatcgctcg
     4561 tatggcatga agctcggcga accatttgaa cttgaaccag gagcggaaaa agcaatcaca
     4621 cttccatttt atgcagatgc ttactattct acgactgaaa aacttcgggt tagaaatttt
     4681 tccagcactg tttcatttac tgtaaacaag aatggtagtg tgaccgatgc agaaaatgta
     4741 gaagttcaaa agaataccgt acaaacacaa gaagcaggtg tagtctggta cattaaaaac
     4801 ttgggcgata aaaaagctca ggttttagtt accagctccg ctagtgatta aaaaattcat
     4861 caaaaacaaa agcgacccct ttcataagga agggatcgct tttttctttt gtttaattac
     4921 ggattcgtat tgaatccttt aattttaacg ggcgggatag cttgggtttt tattgtttgc
     4981 tgttcgacat caactccttt gatgccaaag acgtttacga ggaggttaat aatcccaagc
     5041 gcacttacca taatgaccat accgacaata ccccagagca tgtgctgctg cccggttttt
     5101 cgtgcttcag cattggcagc attttttata aaggcaacga gcccccacaa aaagtacgct
     5161 agggcaagag aaaatgccaa gataatgagc gggttgagga taaacttgtt gagtttaaag
     5221 acaagttcct gtatcccctc tgcggcatag agggtagttt caaacatggg tgtttagata
     5281 caacttccgg ctggtccgat tgctcgagtt cctggaggac aaggaagacc aaggttactg
     5341 ccggaaccac gctgaacgcc aagtgtatta ccaacgaagt agataattcc ccagactgaa
     5401 acaataacaa agagaccaat aatacctgca accatctgct tacgtttatc ggcacgcttt
     5461 gctgcatcag cagtggtgat gaatccgatg actgtccaaa taaagtagag tgttgcaacg
     5521 atcatcaaga aggtaacacc catgcgtgcg tactcaaata cttttgcaac ccaaaaatcg
     5581 atctgaccag tattctgtgc aaatgccgga gcggcaagcg caaaagaact cagggtaaga
     5641 aacagagtaa agaatttatt tttcatgaaa ctaatttaca attgattgag ctactaatac
     5701 tacctacc
//
LOCUS       DATIBQ010000021         2464 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U16294
            k141_569022, whole genome shotgun sequence.
ACCESSION   DATIBQ010000021 DATIBQ010000000
VERSION     DATIBQ010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935789
            Sequence Read Archive: SRR25158331
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2464)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2464)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:58:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 637
            CDSs (total)                      :: 603
            Genes (coding)                    :: 601
            CDSs (with protein)               :: 601
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2464
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_569022"
                     /isolate="SMAG_U16294"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..206
                     /locus_tag="VLB02_00645"
     CDS             <1..206
                     /locus_tag="VLB02_00645"
                     /inference="COORDINATES: protein motif:HMM:NF014624.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HSE56580.1"
                     /translation="GLQEMVTKGAYCIDVITQTSAVKQGLSNIEDVLLEHHLSHCVVA
                     QIKTGESAKATKEIIKVYRLKRK"
     gene            211..>2464
                     /locus_tag="VLB02_00650"
     CDS             211..>2464
                     /locus_tag="VLB02_00650"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012465837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HSE56581.1"
                     /translation="MDTKTYTIKGMHCASCAGIIEKTFKKAAGVHAAEVNYGTETAKL
                     TFDTSKTNPHRLSQKIEKLGYSLAIPASAEAMGMSASEHAAHLGLNQSKQEKLAEVAD
                     MKSKIMSVIPLALFSIVLMGWDILVRYNVVSVMNHTTAEFFHHLLPLFATYTLFVVGK
                     PYLLGFYRFLRYGKANMDTLIGIGTSVAFVYSFIVTAFEEVLRPFINVAYTYYDVTIV
                     VITFIALGKYLEARSKIKTGDAIEKLLTLQAKTALVIRDGKEVEISVNEVKHGDSIII
                     KPGAKIPVDGVITKGASFVDESLVTGEPMPVQKHVGDSVVSGTINTNGSFTFKATKVG
                     SETLLAQIIKMVEEAQGSKAPIQALADKISSIFVPITLGIAFLTLGAWLFLGTGSLGF
                     SQALSYGLVSFVGVLVIACPCALGLATPTAIIVGVGKGAKEGILIKDAATLETLHKVD
                     TVVVDKTGTITKGKPALVDIQNLSPLANDALVSILASLEKKSEHPIAQAIVSYAQEKN
                     ISLSEVSQFENLEGRGIRGEINGTIYFVGNTKLANDLQISFDTTKLNEFTAQGKTPVI
                     LATKENILGFVMVADEVKAESKQAIADLHTLGIQVVMLTGDDESTARYIASLVGIDAV
                     IAHVLPQDKLVKIKELQSQGRVVAMAGDGVNDAPALAQANVGIAMSTGTDVAIASAGI
                     TLLGGDISKLVKAIKLSKMTMRGIKQNLFWAFIYNIVGIPLAAGVFYPLFGWLLSPGF
                     AGLAMALSSVS"
BASE COUNT          696 a          514 c          608 g          646 t
ORIGIN      
        1 gcgggttgca agagatggtt acgaagggtg catactgtat tgatgtcatt acgcagactt
       61 cagcagtaaa gcaggggtta tcgaatattg aagatgtact gctcgagcat cacttaagcc
      121 actgcgtggt tgctcaaatc aaaacaggcg agtctgcgaa agcaaccaaa gaaattatta
      181 aggtataccg cttgaagcga aaataattat atggacacaa aaacgtacac tataaaagga
      241 atgcactgcg cctcgtgtgc gggcattatt gaaaaaacgt tcaagaaagc agcgggcgtg
      301 cacgctgctg aagtaaatta cggaactgaa acagcaaagc ttacctttga tacctctaaa
      361 acaaatccgc atcggctttc gcaaaaaata gaaaagctcg gctatagcct cgctattccc
      421 gcatcagctg aagctatggg tatgtcggca agcgaacatg cagcgcacct cgggctcaat
      481 caatcaaagc aagaaaaact cgccgaggta gccgacatga aatcaaaaat catgtcagta
      541 atcccgctcg cgctttttag tattgttctt atgggctggg atatattggt gcgatacaac
      601 gtagtttctg taatgaacca caccacagcc gagttttttc atcacctcct tcctctcttt
      661 gcaacctaca ccctatttgt ggtgggtaag ccatacctgc tcgggttcta tcggtttctt
      721 cgctacggaa aagcaaatat ggatacgctc attggtattg gaacatctgt tgcgtttgta
      781 tacagtttta ttgttaccgc gtttgaagag gtactccgac cattcataaa cgttgcgtat
      841 acttactacg acgttactat tgtggttatt acatttattg cacttggcaa gtatcttgaa
      901 gcgcgctcaa aaataaaaac aggtgatgct attgaaaaac tgctcacgct tcaagctaaa
      961 acagcgttag ttattcgtga tggaaaagag gtggagattt cggtaaatga agtaaaacac
     1021 ggagatagca ttatcataaa acccggggca aaaattccgg ttgatggtgt cataaccaaa
     1081 ggtgcatcgt ttgttgatga atctttggtc actggagaac cgatgcccgt acagaaacat
     1141 gttggagaca gtgttgtctc gggtactata aatacaaacg gatcgtttac ttttaaagct
     1201 accaaggttg gctcagaaac gctgctcgcc cagattataa agatggttga agaggcgcag
     1261 gggagcaagg ctccaattca agcacttgcc gacaaaattt caagtatttt tgtaccgatt
     1321 acgcttggta ttgcgttcct tactctggga gcgtggctct tcttgggtac tggttcgctc
     1381 gggttttcgc aggcgctctc gtatgggctc gtctcgtttg tgggggtact ggttattgca
     1441 tgcccctgtg cgctcggact tgcaacgcca actgcgatta ttgttggggt tggaaagggc
     1501 gcgaaagagg gaatactgat aaaagatgca gcaacactcg aaacattaca taaggtagat
     1561 acggtggtgg tagacaagac aggtactatt accaaaggca agccggctct ggttgatatt
     1621 caaaatcttt cgcctctagc caatgatgcg ttggtgtcta ttctcgcttc gcttgagaaa
     1681 aaatcagagc atcccattgc tcaggcaatt gtaagctacg cacaagaaaa aaatatctct
     1741 ctatcagaag tctcacagtt tgagaactta gaaggcagag gaatcagagg agaaatcaat
     1801 ggcactatct attttgtggg gaatacaaaa cttgcaaacg atctccaaat ttcttttgat
     1861 accacgaaac ttaacgaatt taccgctcag ggaaaaacac cagtcatact cgcgaccaaa
     1921 gaaaatatcc tcggttttgt gatggttgcc gacgaggtaa aagcagaatc aaagcaggcg
     1981 attgctgacc tccacactct tggtatacaa gtggtgatgc tcaccggtga tgatgaaagc
     2041 actgcacggt acatagcatc cctggtggga attgatgcag ttattgccca cgtattgccg
     2101 caggataaat tagtgaaaat aaaagaactc cagtcgcagg gcagggtggt ggcaatggcg
     2161 ggtgatgggg taaacgatgc accggcgctg gcgcaagcca atgtgggtat tgcgatgagt
     2221 accggcactg atgtagcaat tgcatctgcg ggtattactc ttcttggtgg tgatatttca
     2281 aaactggtga aggcgatcaa actttcaaag atgacgatgc gcggcattaa gcaaaacctt
     2341 ttctgggcgt ttatctataa cattgttggt attccgcttg cagctggagt tttctacccg
     2401 ctctttggct ggctgctcag cccggggttt gcagggttgg caatggcgct ctcgtctgtg
     2461 tcgg
//
LOCUS       DATIBQ010000022         9720 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U16294
            k141_600771, whole genome shotgun sequence.
ACCESSION   DATIBQ010000022 DATIBQ010000000
VERSION     DATIBQ010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935789
            Sequence Read Archive: SRR25158331
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 9720)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9720)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:58:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 637
            CDSs (total)                      :: 603
            Genes (coding)                    :: 601
            CDSs (with protein)               :: 601
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9720
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_600771"
                     /isolate="SMAG_U16294"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..963)
                     /locus_tag="VLB02_00655"
     CDS             complement(<1..963)
                     /locus_tag="VLB02_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HSE56582.1"
                     /translation="MSKDVPEEKPIASQVIPVNIVKEMRDSYLDYAMSVITARALPDV
                     RDGLKPVHRRILYSMYENGLTASAKFRKSAFIVGDVLGKYHPHGDTSVYDAMVNMAQE
                     FSLRYPLVKGQGNFGSMDGDNAAAMRYTEAKMSKIAGELLRDIDKDTVDFRPNYDATR
                     KEPVVMPTVLPNLLLNGTLGIAVGMASNIAPHNLNEIVEACLYVIEHDDATSEDLAEI
                     VQGPDFPTGGLVFGSSDILHALSTGRGGVTCRGEAEIVETKSGVYQIIITSLPYRVVR
                     SSFVESIANLVQEKKLEGIKGLDDLSAVDTRVVVTLKSGVNPQKV"
     gene            complement(990..1193)
                     /locus_tag="VLB02_00660"
     CDS             complement(990..1193)
                     /locus_tag="VLB02_00660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56583.1"
                     /translation="MIEAIKAKPAAERKRIALTVTFTIALVLVIIFFFYLQWQFSKAP
                     AEPGTAWDGFRRLQTSVSETLKK"
     gene            complement(1205..1840)
                     /locus_tag="VLB02_00665"
     CDS             complement(1205..1840)
                     /locus_tag="VLB02_00665"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HSE56584.1"
                     /translation="MVITYFGKECFKITLGDLTIALNPVSKSSKALAKTPRFGADIVL
                     STTNHPDYNGVEQMSFGEREPFVIDGPGDYEIKGLFFKGALSKIVIGGKEYVNTVYGF
                     ELDGIKIAFLGALADESTLTAEAKQIASSADLIFVPAGGGEVFDPAAAYKLAVSFDPN
                     IIVPMDADTEHLKRFLKEGGQEKNETLDKLTLKRRDLDGKEAYIAVLEPQS"
     gene            2098..3219
                     /locus_tag="VLB02_00670"
     CDS             2098..3219
                     /locus_tag="VLB02_00670"
                     /inference="COORDINATES: protein motif:HMM:NF012784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HSE56585.1"
                     /translation="MAKKVTEEQEEVITAAPEADTQNDPMRPKLEDLVEGPVILVGKA
                     AVYVDLKPFGTGIIYGREFINARDLIKKINAGDVIKAKVVEVENDDGYVELSLKEAKQ
                     ALIWSDAEKAIKTKEIFELPVKEANKGGLIVEWQGVQGFLPASQLKPEHYPRVEDSDK
                     EKILRELKKIIGKKLAVTIISANPKEGKLIFSEKESNPEERQEVISKYTVGDELDCEV
                     TGIVDFGIFLKIEDGLEGLVHISEIDWGLVEDPRTMFKLKDKVRAKIIDIKDGKISLS
                     IKALKENPWKEFEGKIKKGEVVKGIVIKYNKHGALISIKEGVAGLVHNSTFGSEEALR
                     KELLLGKTYPFTVTLFEPKEHRMTLVFGDKLPPEEEKEA"
     gene            complement(3258..3824)
                     /gene="pth"
                     /locus_tag="VLB02_00675"
     CDS             complement(3258..3824)
                     /gene="pth"
                     /locus_tag="VLB02_00675"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HSE56586.1"
                     /translation="MYIFIGLGNPGKEYERTRHNTGKMAVEALAGGWGVSFEQNKVFN
                     AQVVKHDGALLAIPNTYMNDSGSVVKVLKREYADAELVVLYDDIDIPLGTVKCSFNRG
                     AGGHNGVQSIIDSLGTNEFFRIRIGVRPIDERLLPQIAPPTGFQDFLLSNFTPLEDEG
                     LKEGITKAAAIAKFLLTHSREETMNIYN"
     gene            3994..4563
                     /gene="lepB"
                     /locus_tag="VLB02_00680"
     CDS             3994..4563
                     /gene="lepB"
                     /locus_tag="VLB02_00680"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HSE56587.1"
                     /translation="MDPAPNRPSKLKSFLEGALYFASAFVLAVIIQTYIARPFIVNGQ
                     SMYPTFKNGEYLIVDEISYRLGEPERGDVVVFKAPPTPEKFFIKRIIGLPGETVISKN
                     GKVTIVNAEHPEGFTLDESFLKDMTTGTFEAKVPEDAYFVMGDNRTGSFDSRAWGTLP
                     KENLRGRALLRLLPFGRISYKPGDVSFEN"
     gene            4566..5864
                     /gene="hisS"
                     /locus_tag="VLB02_00685"
     CDS             4566..5864
                     /gene="hisS"
                     /locus_tag="VLB02_00685"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HSE56588.1"
                     /translation="MEKKKEKKNETLTTPKGMRDIIGDDYYKFQGFFEKAQEVAVYFG
                     FKPIETPIIEYEEVFTTAVGIGTDIVDKEMYTLKTKGGDHFALRPEQTAALMRAYIEH
                     GMQNQPQPVLFYHSGPVFRHDKPQRGRYRQLYQFDVDCIGSEKSIVDALVIKTIVTIL
                     EEVGAKNFVVNINSIGDKECRPTYLRELVNYYKKHLAALPALDRERLKTNPLRILDSK
                     EPVTIELNKEAPDAVSFLTGNAKKHFKEVLECLDEMNIPYTIDKTLVRGLSYYTHTVF
                     EIVELDGGEGGTPLSIAGGGRYDYLGRQLGSRKDVPGMGGSIGIDRVVEAPWYKAVAP
                     RIMKKPKVYFIQVGFEAKLKSLNVIEILRKAHIPIMQSLSKDSLSAQLGVAEKSGVPY
                     VIIFGQKEAVENSVIVRHMETHSQDTVKLDKLLEYFKNLK"
     gene            5868..6371
                     /gene="def"
                     /locus_tag="VLB02_00690"
     CDS             5868..6371
                     /gene="def"
                     /locus_tag="VLB02_00690"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:NF013492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HSE56589.1"
                     /translation="MKDIIQDPDPVLRQAAAAVPVSEITTKKIQGIVAAMQEALANEE
                     DGVAIAAPQIGVSMRLFVVSKKVLKDAPADMVFINPELIRLGKQKKFVHEGCLSVRWK
                     YGLVKRATTATVRAYNMQGNQFQMSARGLLAQVFQHETDHLDGILFIDSAKEIEDIPP
                     HHEPHTV"
     gene            6352..7200
                     /gene="fmt"
                     /locus_tag="VLB02_00695"
     CDS             6352..7200
                     /gene="fmt"
                     /locus_tag="VLB02_00695"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HSE56590.1"
                     /translation="MNPTPSNPRFAFFGTPVFSTFVLHELVSNGYVPNLIVTTPDKPA
                     GRGLEIVQSPVKKFALEQNIPVLQPEVLNEHFIAELKKRNIDLLVVAAYGKILPSAVL
                     DIGKVPPLNVHPSLLPKYRGTSPVESQILAGEEEVGVTIIQMDEKMDHGPIVAQEKVS
                     VSKQSTRDELNRILWTRGGQILSEIIPSWIAGNKTPTPQDHSQATFTKKISKEDGEVS
                     LSDDPVILDRKFRAYHPWPGLYFYTNRNGTRTRVKIKTARWENEQFIIETVIPENGKP
                     MPFSAL"
     gene            complement(7201..7593)
                     /gene="raiA"
                     /locus_tag="VLB02_00700"
     CDS             complement(7201..7593)
                     /gene="raiA"
                     /locus_tag="VLB02_00700"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HSE56591.1"
                     /translation="MHITFKSTNIELTPTLRDYTEKKLQVLEKFSKGNGQAAVELEKT
                     TNHHKQGDIFRAEANVTLGTGAQYRAVSEKEDLYEAIDDMKDELVRELTAGKGKERAL
                     WKRGALAVKGMMRGSYEVIKGFRFRKRK"
     gene            complement(7645..7866)
                     /locus_tag="VLB02_00705"
     CDS             complement(7645..7866)
                     /locus_tag="VLB02_00705"
                     /inference="COORDINATES: protein motif:HMM:TIGR00251.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HSE56592.1"
                     /translation="MEVRIRVTTNARGTACTELSPGVFSVSVSEKPIDGKANDAVCRV
                     LAAYFETVPSAVRIIRGHRSPNKIARILL"
     gene            7962..8342
                     /locus_tag="VLB02_00710"
     CDS             7962..8342
                     /locus_tag="VLB02_00710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56593.1"
                     /translation="MSKILATVTVFIGIFILSATPVAAQYYQNQNYGYGYVHDYGYNT
                     QPCCLSYAWQDPYVTSQYRPMYLPPQQPIYTGGWNEPYYYNQNYILPNRYSYGSNYGF
                     GYQPYDNYNNRGWDRGYVTLGFGW"
     gene            8399..8470
                     /locus_tag="VLB02_00715"
     tRNA            8399..8470
                     /locus_tag="VLB02_00715"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8431..8433,aa:Asn,seq:gtt)
     gene            complement(8512..9096)
                     /locus_tag="VLB02_00720"
     CDS             complement(8512..9096)
                     /locus_tag="VLB02_00720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56594.1"
                     /translation="MEIFKQKNLFAYLYSKLTAVPTQRKKEKILLHLLSEYFRLSEIC
                     ASKAKTDFVTEFTLPIKLRNEVLVESVKLLLLAMSKKDYLNLEGMEKILEDPEHVEIT
                     EDGIRYKTDTARLVLDFELIEMNSIMDGHERYVLEQKIMYYDLIQICSSNAITGIELF
                     IGKYCNNYFVESLLEWVIEKMKKRIGENNFSVSY"
     gene            complement(9248..>9720)
                     /locus_tag="VLB02_00725"
     CDS             complement(9248..>9720)
                     /locus_tag="VLB02_00725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56595.1"
                     /translation="SGDHFVVCINKNLRMVEQRYTIAHEVAHILHSFWFNYSYGRPGE
                     RPFIPGHNRYLSLEEELCDYMAIRLLAPEKQVTRWIQKYYTLPCQLSLFDEVQRGEGI
                     ALFSLAKHFCVPVKEMGLWIKQIATTRTIGVSSREDTAPFPSVRECIGKIALTT"
BASE COUNT         2786 a         2336 c         2062 g         2536 t
ORIGIN      
        1 caccttctgt ggattcaccc cactcttaag tgttaccacc acccgagtat caaccgccga
       61 aaggtcgtcg agccccttaa tgccttcaag ttttttctct tgtaccaaat ttgcaatcga
      121 ttcaacaaaa ctcgagcgca ccacccgata cggcagcgag gtaataataa tctgatacac
      181 accgctcttt gtttcgacaa tttccgcctc gccgcggcag gttactccgc ctcgccccgt
      241 tgaaagcgca tgcaaaatat cgctgctgcc aaacacgagc ccgccggtcg gaaaatcggg
      301 accttgcaca atttcggcta aatcttcgct cgtcgcatcg tcatgctcaa taacatacag
      361 gcaggcttca acgatttcat ttaaattgtg cggagcaatg ttgcttgcca tacctactgc
      421 aataccaagc gtgccgttta acagcaagtt tggaagcacg gtcggcatca ccaccggctc
      481 tttgcgggtt gcatcgtagt ttgggcgaaa gtccaccgta tctttatcga tatcgcgcaa
      541 gagctcgcca gcaattttgc tcatttttgc ctcggtgtag cgcattgccg cagcattgtc
      601 gccgtccatc gaaccaaagt ttccctgccc tttcaccaac ggatagcgca agctaaattc
      661 ctgtgccata tttaccatcg catcgtagac actcgtgtcg ccgtgcgggt ggtacttacc
      721 gagcacatca ccgacaataa acgcactctt gcgaaacttc gcactcgccg tgagaccatt
      781 ctcgtacatc gagtacagaa tgcggcggtg cactggcttc aaaccatcac gaacatccgg
      841 cagggcgcgc gcggtaatca cactcatcgc gtaatccagg taggaatcac gcatttcttt
      901 aacaatattg accgggataa cctgcgaggc aatcggcttt tcttcaggga cgtctttaga
      961 cataaaggga ttttagcata caaataaggt tattttttga gtgtttcaga aacagaggtt
     1021 tgtaggcgcc tgaagccgtc ccaggctgtt cccggctcgg caggagcttt tgaaaactgc
     1081 cactggaggt aaaaaaagaa gatgataacg agtacgagcg cgatagtaaa cgttaccgtg
     1141 agcgcaatcc gcttccgctc tgctgccggt tttgctttaa ttgcttcaat cacctttcgc
     1201 atggctaact ctgcggctcg agcactgcaa tgtacgcctc tttgccgtcc aaatcacggc
     1261 gcttcaaggt gagcttgtcg agtgtttcat ttttctcctg cccaccctct ttcaaaaacc
     1321 gcttcagatg ctcggtgtca gcatccatgg gcacaataat atttggatca aaggatacgg
     1381 caagcttata tgctgctgcc ggatcaaata cctcaccgcc gccagccgga acaaaaataa
     1441 ggtcagcact cgaggcaatc tgttttgctt cagcagtaag ggtgctctcg tcagcaagtg
     1501 cgccgagaaa ggcaatttta attccatcga gctcaaaacc gtacacggtg tttacatatt
     1561 ctttaccacc aataactatc tttgaaagcg cccctttaaa aaagagccct tttatctcgt
     1621 aatcgcccgg accatcaatc acaaacggct cccgctcgcc gaaagacatt tgttctacgc
     1681 cattataatc agggtgattg gtagtagaga gcacaatatc ggcgccaaac cgaggcgtct
     1741 tagcgagcgc ttttgatgat ttactcaccg gattgagggc aatcgttagg tctccgagtg
     1801 ttattttaaa gcactccttg ccaaaatagg ttattaccat aaggctattt tagcacaaac
     1861 ataagttttt taaaagatta aaaaagaaca ttgctgcact tgctatttgg tcaatatagt
     1921 gatagttttt taaggcaaat tcgtttgcat taataaattc ccttttggaa taacctcgca
     1981 cttccctgtt ttgactgagc aagtgcgaaa tctaaaaggt ggctccgctc gaaaggcgga
     2041 ataagccgcc tttggcggca attttctttg tcccgccgaa gctggcgaag gaggattatg
     2101 gcaaaaaaag taacagaaga acaagaagaa gttataacag ctgcaccgga agctgatact
     2161 cagaacgatc cgatgcgacc aaaacttgaa gacctcgttg aaggtccggt tattttggtg
     2221 ggcaaagcag cagtgtatgt tgatcttaaa ccattcggta ccggtattat ttacggacgt
     2281 gaatttatca atgctcgcga cctgattaag aagataaacg cgggagacgt aatcaaggca
     2341 aaagtggttg aagtcgaaaa cgacgacggc tacgttgagc tctctctcaa agaagcaaag
     2401 caggcgctca tttggagcga tgctgaaaag gcaatcaaaa caaaagaaat ttttgagctc
     2461 ccggtaaaag aagcaaacaa gggcgggctt attgttgagt ggcagggcgt gcaagggttc
     2521 ttgcctgcat cccagctcaa gcccgagcac tacccgcgtg ttgaagactc tgacaaagaa
     2581 aaaatccttc gtgagcttaa gaaaattatc ggcaaaaagc ttgcggtaac gattatttct
     2641 gcaaatccaa aagaaggaaa gcttattttc tctgaaaagg aatcaaaccc tgaggagcgc
     2701 caggaagtaa tcagtaagta caccgtgggc gacgagctcg actgcgaggt aaccggtatt
     2761 gttgatttcg gtatcttcct gaaaatcgaa gacgggcttg aaggcctggt gcacatctca
     2821 gaaatcgact ggggcctggt tgaagacccg cgcaccatgt tcaaactgaa ggataaagta
     2881 cgcgcgaaga tcatcgacat taaagatggc aaaatctcac tctctatcaa ggcattaaaa
     2941 gaaaacccat ggaaggaatt tgagggcaaa atcaagaagg gcgaagtagt aaaagggatt
     3001 gtgattaaat acaacaagca cggtgcgctc atctctatta aagaaggggt tgctggcttg
     3061 gtacacaact caaccttcgg cagcgaagaa gcactccgca aagagctgtt acttggaaaa
     3121 acctacccat tcactgttac tctctttgaa ccaaaagagc accgcatgac cttggtgttt
     3181 ggcgacaaac tcccaccgga agaagaaaag gaagcataaa taataaaaaa cactttctaa
     3241 ataaaagtgt tttttattta gttgtagata ttcatcgtct cttccctact atgcgtgagc
     3301 aaaaacttgg caattgcagc cgctttagta attccctctt tcagcccttc atcttcaagc
     3361 ggggtgaaat ttgagagtaa aaaatcttgg aagccggttg gtggagcgat ttgcggcagc
     3421 agtcgctcat caatgggtcg cacgccaatg cgaatgcgga aaaattcgtt cgtgccgagt
     3481 gaatcaataa tcgactgtac cccattgtgc cccccggcac cacggttaaa cgagcacttc
     3541 acggtaccaa gcggaatatc aatgtcatcg tagagcacca ccaactctgc atcggcatac
     3601 tcacgcttca atacttttac taccgagcca gaatcgttca tgtaggtatt agggatcgcc
     3661 aagagtgccc catcgtgctt taccacctgc gcattgaaca ctttgttctg ctcaaaggat
     3721 acaccccacc ctcctgcaag cgcttctacc gccatcttcc ccgtattgtg ccgcgttcgc
     3781 tcgtactctt tgcccggatt cccgagaccg ataaatatat acatgcaaaa attatacaca
     3841 aataatcaaa tttcaataac caacagccaa acaatggcta aaaagttttg gactttagat
     3901 tattggtata ttggaaattg cttgattatt aggacttggt tattgattat tgccccacag
     3961 ggggctttgt attacaatgc gcgtaccaaa cctatggatc cagctcctaa tcgcccctca
     4021 aaactaaagt cttttcttga aggagctctt tattttgcca gcgcgtttgt gctcgccgtc
     4081 atcatccaaa cctacatcgc tcgccccttt attgtaaacg gacaatcgat gtaccccacc
     4141 tttaaaaatg gcgaatactt gattgttgat gaaatttcat accgcctagg agaaccggaa
     4201 cgcggcgatg tagtggtgtt taaagcaccg ccaactcctg aaaaattctt tatcaagcgt
     4261 attatcggtt tgcctggcga aactgttatc agcaagaacg gaaaggtaac tattgtaaac
     4321 gccgaacacc cagaaggctt taccctcgat gaatcatttt taaaagatat gactacaggt
     4381 acgtttgaag caaaagtccc cgaagatgcg tacttcgtga tgggcgataa ccgcaccggc
     4441 agttttgact ctcgtgcgtg gggaacgctg ccgaaagaaa acctccgcgg ccgagcactc
     4501 ctgcgcctct tgccgtttgg ccgcatcagc tacaaaccag gcgacgtttc gtttgaaaac
     4561 taagtatgga aaagaaaaaa gaaaaaaaga atgaaaccct gacaaccccc aaaggaatgc
     4621 gcgacattat tggcgatgat tactataaat tccagggctt ttttgaaaaa gcacaagagg
     4681 tagcggtgta ctttggcttc aagccgattg aaacaccgat tattgagtac gaagaagtat
     4741 ttactactgc tgttggtatt ggtaccgaca tcgtcgataa agaaatgtat actttaaaaa
     4801 ccaaaggtgg cgaccacttt gccttgcgcc ctgaacaaac tgccgcactc atgcgcgcat
     4861 acatcgagca cggcatgcaa aaccagccgc agccagtact cttctaccac tcgggtccgg
     4921 tattccggca cgataaaccg cagcgcgggc gctaccggca gctctaccaa tttgatgttg
     4981 actgcattgg cagcgaaaaa agtattgttg atgcactggt aataaaaaca atcgttacaa
     5041 ttcttgaaga agtgggcgca aagaattttg tcgtcaacat taattccatc ggcgacaaag
     5101 agtgccgccc gacgtacctc cgcgagctcg tcaactacta caaaaaacac ctcgctgccc
     5161 tccccgcact tgaccgagaa cgattaaaaa caaatcccct gcgcattctt gattctaaag
     5221 agccggttac cattgaactg aataaagaag ctcctgatgc agttagcttt ttgacgggca
     5281 acgcaaaaaa acattttaaa gaagtacttg aatgcttgga tgaaatgaat atcccctaca
     5341 caattgataa aacactcgtc cgcgggctct cgtactacac ccacaccgtg tttgagattg
     5401 tcgagctcga tggcggtgaa ggcggcacac cgctctcaat cgctggtggt gggcgctatg
     5461 attatttagg acgacaactt ggaagccgga aggatgtccc cggcatgggc ggctcaatcg
     5521 gtattgatcg cgttgtagaa gcaccgtggt acaaagcggt cgcaccacgg attatgaaga
     5581 agccaaaggt gtactttatt caagttggtt ttgaggcaaa actcaagagt ttgaacgtga
     5641 ttgaaattct gcgaaaagca cacatcccga ttatgcagtc gctctcaaaa gacagtctct
     5701 cggcacagct gggcgttgct gaaaaatcag gtgttcccta cgtgattatt tttgggcaga
     5761 aggaagcagt tgaaaactca gtgattgttc gccatatgga aacccactcg caagataccg
     5821 tcaaactcga caaactgctc gagtacttca aaaatttaaa gtagtctatg aaggatatta
     5881 ttcaggatcc ggatccggta ctgcggcagg cagctgcggc agtaccagta tctgaaatca
     5941 ccacaaaaaa aatacagggt attgttgccg caatgcaaga agcgctcgcc aacgaagaag
     6001 acggtgttgc tattgccgca ccgcaaattg gtgtttcgat gcggttgttt gtagtgtcaa
     6061 aaaaagtgct caaagatgca cctgccgata tggtattcat caaccccgag cttatccggc
     6121 tcggaaaaca aaaaaagttt gtgcacgagg gctgtctttc ggtgcgctgg aagtacggct
     6181 tggtaaagcg tgcaacaacc gcaacagtgc gagcttataa tatgcagggt aaccaattcc
     6241 aaatgagcgc ccgtggtctg ttggcgcagg tattccagca cgaaaccgac cacctcgacg
     6301 gcatactttt tattgactct gcaaaagaaa tcgaggatat ccctccacat catgaacccc
     6361 acaccgtcta atccgcgttt tgcttttttt ggcacgcccg ttttttccac cttcgtactt
     6421 cacgaacttg tctctaatgg ctacgtgcca aaccttatag tcacaacccc agacaaacca
     6481 gccggtcgcg gtcttgagat cgttcagtca ccagtaaaaa aatttgccct cgagcaaaac
     6541 attcctgtcc ttcagccaga agtacttaat gagcatttta ttgcagaact aaaaaaaaga
     6601 aatatcgatc tccttgttgt tgcagcatac ggaaaaatac ttccttctgc tgttttggac
     6661 ataggaaaag tgcccccgtt aaatgttcac ccatcgttgc tcccaaaata tcgcggcact
     6721 tcccctgtcg aaagtcagat tcttgccggc gaagaagagg taggtgtaac cattatacaa
     6781 atggatgaaa aaatggatca tggtccgatt gtggcacaag aaaaagtttc agtttcaaaa
     6841 cagagcaccc gagacgaact gaataggatt ttatggacga ggggcggtca aatactctct
     6901 gagattattc cgagctggat agcgggaaat aagacaccaa ccccgcaaga tcattcgcag
     6961 gcaacgttca ctaaaaaaat ctctaaagaa gatggtgaag tatctctctc tgatgacccc
     7021 gttattcttg atcgaaaatt tcgagcatac catccatggc ctgggctcta tttttatacc
     7081 aaccggaacg gcacccgcac cagagtcaaa ataaaaaccg cccgttggga aaacgagcag
     7141 ttcatcatcg aaaccgttat ccctgaaaac ggcaaaccaa tgccattttc agcgctgtaa
     7201 ctatttccgt tttcgaaacc ggaacccttt tattacttcg tagctgccgc gcatcattcc
     7261 ttttactgcc agggcaccac gcttccagag tgctcgttcc ttccctttcc cggcggtcag
     7321 ctcacgtacg agctcatctt tcatgtcatc gatcgcctcg tataaatcct ctttttcaga
     7381 cactgcccgg tactgcgcac cagtgccaag tgttacattt gcttcagccc gaaaaatatc
     7441 gccctgctta tggtgattcg ttgttttttc aagctcaacg gcagcctgtc cattaccttt
     7501 agagaatttc tcaagtacct gaagcttctt ttcagtatag tcacggagag tgggggtaag
     7561 ctcaatgttg gttgatttga atgttatatg catatatggt ctcctcgttt acgcttataa
     7621 actcctagta acaattttag tgtatcacaa gagaatgcgg gcaattttgt tcgggctgcg
     7681 gtgcccgcgg ataattcgca ccgcgctcgg cactgtttca aaataagcag ccagcacccg
     7741 acacactgcg tcatttgctt ttccgtcaat tggtttttct gaaactgaaa cagaaaacac
     7801 tccgggcgaa agttccgtac atgccgttcc gcgagcatta gtagtaaccc gaatccgtac
     7861 ttccattacc gtttaattat actagataat agttttttca tcattttcgt accatatgtc
     7921 aatatcttgc tcgctacact taagcatatt agtatacgcg tatgagcaaa atactagcaa
     7981 cagtaacagt gtttattggt atatttatac tttctgctac cccagtagcg gcacagtact
     8041 accaaaacca aaattatggg tacgggtacg tccatgacta cggttacaat acccagccct
     8101 gctgcttgag ctatgcctgg caagacccgt acgtaacatc gcagtaccgc cccatgtacc
     8161 tgcccccgca gcagcctatt tacaccggcg gatggaatga gccgtactac tataaccaaa
     8221 attacatact gccaaaccgg tactcgtatg gcagtaacta cggattcggc taccagccgt
     8281 acgataatta caataatcgc ggctgggaca gaggctacgt taccctcgga tttgggtggt
     8341 agaaggaagc aaataaaagt gctataatcg ctctgcgttt ttcacgcatc aactagattc
     8401 ccctgtggcg cagcggtagc gcagtcgcct gttaagcgat tggtcgtagg ttcgaatcct
     8461 accgggggag caaaaaaaag agcaccacaa ccattggtgc tcttttaaag attaatacga
     8521 aacagaaaaa ttattttctc ctatcctctt tttcattttt tcaattaccc actctaacaa
     8581 agattcaaca aaataattat tacaatactt accgataaac aactcaatgc ctgttatagc
     8641 atttgaacta caaatttgta ttagatcata atacattatt ttctgctcca agacataacg
     8701 ttcatgacca tccataatgc tattcatttc tatcagttca aaatcgagta ctaatctcgc
     8761 tgtatcagtt ttatagcgta taccgtcttc tgttatctcc acatgttctg gatcttcaag
     8821 aattttttcc atgccttcca aattaaggta atccttttta ctcattgcaa gtaaaagaag
     8881 cttaaccgat tctactaaaa cttcgtttcg caattttatc ggcaatgtaa actcggtaac
     8941 aaaatctgtc ttggcttttg aggcacagat ctctgagagt cggaaatact ctgagagtag
     9001 gtgaagtaat attttttcct tttttctttg tgtaggtacc gccgttaatt tggaatataa
     9061 atacgcaaaa agatttttct gtttaaatat ttccatataa taagtttttt tattactata
     9121 atataaaaaa ctatacatgt caaatataat tgactaccat ctgatgagta gaaactactg
     9181 cagatacaaa caaataattc cgaacatcct ctaaggttcg gggtagcgaa atgaaaaagc
     9241 accaacttta tgtagttagt gctatcttcc caatacattc cctgaccgag gggaaaggcg
     9301 cagtatcttc tcgactactg acaccaattg ttctagtggt agctatttgc ttaatccata
     9361 accccatttc tttaaccgga acacaaaaat gttttgcaag cgagaacaaa gctatgcctt
     9421 cacctctttg tacttcatca aacaatgaga gctgacacgg taacgtgtaa tatttttgta
     9481 tccacctggt tacttgtttt tccggagcaa gaagacgtat cgccatataa tcacacaatt
     9541 cctcttcaag cgataagtat cgattgtgtc ctggaataaa tggtcgttct cctggtcttc
     9601 catagctgta attgaaccag aaagaatgga ggatatgggc aacttcgtga gctatcgtat
     9661 accgctgctc gaccatacga agatttttat tgatacatac aacaaagtga tctcctgata
//
LOCUS       DATIBQ010000023         5802 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U16294
            k141_2072331, whole genome shotgun sequence.
ACCESSION   DATIBQ010000023 DATIBQ010000000
VERSION     DATIBQ010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935789
            Sequence Read Archive: SRR25158331
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 5802)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5802)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:58:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 637
            CDSs (total)                      :: 603
            Genes (coding)                    :: 601
            CDSs (with protein)               :: 601
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5802
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2072331"
                     /isolate="SMAG_U16294"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2705
                     /gene="rpoC"
                     /locus_tag="VLB02_00730"
     CDS             <1..2705
                     /gene="rpoC"
                     /locus_tag="VLB02_00730"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HSE56596.1"
                     /translation="LKKLKEIGAPDVILRNEKRILQEAVDALIDNSIASQDSPAMSQA
                     QRRALKSLSDNLKSKQGLFRQNLLGKRVDYSGRSVIVVGPQLRLNQCGLPKHMALELF
                     RPFVISKVISRELAYNIRGANKLIEDGIPEVWAILEEVIKGKYVLLNRAPTLHRLGIQ
                     AFMPILIEGNAIQVHPLVCPAFNADFDGDQMAVYVPLSDTAQAEARTLIAADKNLLKP
                     QNGDPIVSSKMLDIVLGCYWMTSVMEGANGEGKYFASPNEAITAYDFDMVSFRAKIHV
                     LGTNKGKYEQFGGKPFETTVGRLFFNSVLPADFPYLNEDITSKKMVALVDQIIARYGI
                     DGTAPILDKIKDFGYKYATKSGTTWGIDNVIVPKEKYEIVEKHRALEEEVRSQFEEGL
                     LSEEERFSKVIAVWERCKSEIEKILPNTIEKHGSVYDMITSGARGSMGNVTQMAGMKG
                     LIQNTTGRTIDFPIIPSYKEGLSPIEYFITTHGSRKGLADTALNTARAGYLTRRLVDV
                     AQDVVITEDDCGVKAGKRITKEYISGLEIPLSKSIRGRVLSADVKTAAGTTLFKRGHL
                     VSKDDATLIEKEGIEEVFVRTPLVCRTVYGICRQCYGIDLGRGHLVDRGESVGIVAAQ
                     AIGEPGTQLTLRTFHAGGVAGVDITAGLPRVEEIFECRSIKNAAVVAKVGGTVSEIRE
                     EGSEKTIVVLSDKNESKKDSIEYTTPPHRMPIVKVGQDIKKGELLTDGSADIHEVYEY
                     GGKDAAEDYIVREISKVYELQGASIARKHLEIIIRQMFSRRKIKDTGDTSFSPGDVVE
                     TTDLMEENARMRNATEDFREAKADTVILGITQAALHAKSWLSAASFQNTNRILIQNAV
                     KGGVDTLRGLKENVTVGRLIPAGTGFRERKALDVNTFAKEPEQE"
     gene            2751..4475
                     /gene="dnaG"
                     /locus_tag="VLB02_00735"
     CDS             2751..4475
                     /gene="dnaG"
                     /locus_tag="VLB02_00735"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HSE56597.1"
                     /translation="MNSIVEQIKNRLSITDVLSSYVTLIPAGSQFKAKCPFHNERTAS
                     FSISPDRGLYYCFGCGAKGDIFTFVEAFEGVDFNGALQMLADRAGIKITRDSFKHDST
                     TALYEVLEDAASIFEVQLASNSLAKAYLAERGVTEETIKKFRIGYAPDSWDTLARRYS
                     GIKQAVAEQAGLLKPGDHGLYDRFRARIMFPIGDSSGRIVGFSGRMFPDVEGGAKYLN
                     SPETPLFQKSKLLFGFDKAKFDIKRRGFAILVEGQFDLILSHQFGFTNTVATSGTALG
                     EESLDGASSLQTLARLTNNLMLAYDGDEAGKKAMDRAAQVALRLGMNPKVAALPAGSD
                     PADFLRTEGSAAWKEQLRTSEHVVVFQAKDLLAKQLSPHQFAAGLRERVFPYLALIPF
                     AIERVQQLELVASAAGLPSAALQEDFSHFLASRPKDHTASTALHTEPSKKKQQFDTPA
                     LFLGILSWQTGLAEPSIDSALYRDRAEAISFEENRYTLPELTDDTLHAARMYAAAFYS
                     SLTKESLETLLDDQLQHMTVSFLHGLRTQFTVELHAAERAGDESRVTTLLSILHQLSV
                     RLQGLKAP"
     gene            4516..5793
                     /locus_tag="VLB02_00740"
     CDS             4516..5793
                     /locus_tag="VLB02_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006525441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HSE56598.1"
                     /translation="MPQKKKSKVPQKKKVVNKTSKKVQPKKKVAAKAQKKVSPKKIPA
                     KPKVKAKPITIKHPGSSPRREANTEKKLQELQLKADELITKGKKRGFITYDEILKTFP
                     DIENNILFLDELYEKFSTAGVDVLEGGNLLDIDNTEDLSKYTKDSHTFSSVQMYLKDI
                     GQYPLINAAQEKDLARRIIAGDMEAKNLLARSNLRLVVSIAKKYANRTSDLTLLDLIQ
                     EGNIGLFKAVEKFDWTKGFKFSTYATWWIRQSITRALADQSRTIRIPVHMVETISKYK
                     QVMRRLAQDLGRDPLAEEIANEMGVEVEKIHVIEQINQETRSLEQPIGDDEDKSTFGE
                     FIADDKILRPDQEASRRMLQDQIRGVLEELSPKEKRILEMRYGLTDGVQHTLEEVGKE
                     FGVTRERIRQIEAKVHEKLRQNEKIMRLKNYFD"
BASE COUNT         1628 a         1343 c         1439 g         1392 t
ORIGIN      
        1 gcttaaagaa gctcaaagaa attggcgcac ctgatgtgat tctgcgaaac gaaaagcgta
       61 ttctacaaga agcagttgat gccttgattg acaactcgat tgcgagccaa gattctccgg
      121 caatgagcca ggcacagcgc cgcgcgctca agtcgctctc agataacctc aagagcaagc
      181 aagggttgtt ccgtcagaac ctgctcggta agcgcgttga ctattcggga cgttcagtga
      241 ttgtggttgg tccacagctg cgcttgaacc agtgcggatt gccaaaacat atggcactcg
      301 aactcttccg accgtttgtt atctcaaaag taatctcgcg cgaactcgcg tacaacattc
      361 gcggggcgaa caagctgatt gaagatggta tcccagaagt gtgggcaatt cttgaagaag
      421 ttattaaagg gaagtacgtg ctcttaaacc gtgcaccaac actgcaccgg cttggtattc
      481 aggcatttat gccaatcttg attgaaggta atgcaattca ggtgcacccg ctcgtgtgtc
      541 cggcatttaa cgccgacttc gacggtgacc agatggcagt ctatgtgccg ctctctgata
      601 ctgcgcaggc tgaagcgcgt accctgattg ccgctgataa aaaccttctc aagccgcaga
      661 acggcgatcc aattgtttct tcaaaaatgt tggacatcgt gctcggttgt tactggatga
      721 cgtcagttat ggaaggtgca aacggcgagg gaaaatattt tgcgagtcca aatgaagcga
      781 ttacggcgta cgactttgac atggtgtcat tccgcgcaaa gatacatgta ctcggtacca
      841 acaagggcaa gtacgagcag tttggcggca agccgtttga gacaacagtg gggcgactct
      901 tctttaactc ggtgcttcct gctgacttcc cgtacttgaa tgaagacatt acttcgaaaa
      961 aaatggtagc gctcgttgat caaattattg ctcgctacgg tattgatggt acggcaccaa
     1021 tcttggataa aatcaaagac ttcggctata agtacgctac taaatcgggt actacctggg
     1081 gtattgataa tgttattgtg ccaaaggaga agtatgaaat cgttgaaaaa caccgtgcac
     1141 tcgaagaaga agtgcgttca cagtttgaag aaggactcct ctctgaagaa gaacgcttca
     1201 gtaaagtgat tgccgtgtgg gaacgctgca agagcgagat cgaaaagatt ctgccaaaca
     1261 cgattgaaaa gcacggatca gtgtacgaca tgatcacctc aggtgctcgc ggctcgatgg
     1321 gaaacgttac ccagatggcg ggtatgaagg ggttgattca aaataccact ggtcgcacga
     1381 tcgactttcc gattatccca tcttacaaag aaggactttc gccaattgaa tactttatta
     1441 ccacgcacgg atcccgaaaa ggtcttgctg acaccgcact caacaccgct cgcgctggat
     1501 atcttacccg tcgtttggtg gatgttgcac aggatgtcgt gattactgaa gatgattgtg
     1561 gggtaaaagc aggaaagcga atcacgaaag agtacatttc aggacttgaa attccgctct
     1621 caaaatcaat tcgcgggcgt gtactttcgg cagatgtaaa aactgctgct ggcaccacac
     1681 tctttaaacg cggacatttg gtttcaaaag acgatgcaac tttgattgaa aaagagggaa
     1741 ttgaagaggt gtttgttcga acaccgctcg tttgccgaac agtatatggt atctgtcgtc
     1801 agtgttatgg tattgatctt ggtcgtgggc accttgtcga tagaggtgaa tcggtgggta
     1861 tcgttgctgc acaggcaatt ggtgaaccgg gtacccagct cacccttcgt accttccacg
     1921 ccggcggtgt ggctggtgtc gacattaccg cagggttgcc tcgcgttgaa gaaatttttg
     1981 aatgtcgctc tattaaaaac gcagcagtgg ttgcaaaggt gggtggtacg gtatctgaaa
     2041 ttagagaaga gggaagtgag aaaacaatcg tagtactctc ggacaaaaat gaatcgaaaa
     2101 aagattcaat cgaatatacg actccaccgc accgcatgcc gatcgtgaaa gtggggcaag
     2161 atattaaaaa aggtgaactg ctcactgatg gttctgctga tattcatgaa gtgtatgaat
     2221 atggtggcaa agatgccgct gaagactaca ttgttcgtga aatcagtaag gtgtatgaat
     2281 tgcagggcgc atcgatcgca cgcaaacact tggaaattat cattcgccaa atgttctcac
     2341 ggcgcaaaat taaagacact ggtgatactt cattctcacc aggagacgtg gttgaaacca
     2401 ctgacttgat ggaagagaat gctcggatgc gcaatgcaac tgaagacttc cgcgaagcaa
     2461 aagcagacac ggttatctta ggtattaccc aagcagcgct ccacgcaaag agctggctct
     2521 cggcggcatc gttccaaaac accaaccgca ttcttattca gaatgcagtc aagggcggtg
     2581 ttgatacact tcgcggtttg aaagaaaacg ttaccgttgg tcggctcatt ccggcaggta
     2641 ccggtttccg cgaacgaaaa gcacttgatg taaacacctt tgccaaagaa cctgaacaag
     2701 aataaagtct cggtgagtag tgctactata ctagctatca ctgccacatc atgaattcta
     2761 ttgttgaaca gattaaaaac aggctctcaa tcaccgatgt tctttcttca tatgtcaccc
     2821 ttattcctgc aggctcgcag tttaaggcga agtgtccgtt ccataacgaa cggactgctt
     2881 cgtttagcat aagtcccgac cgcggactct actactgttt tgggtgcggc gccaagggcg
     2941 acatctttac ttttgtcgaa gcattcgagg gggtggattt taatggagca ctgcagatgc
     3001 tcgctgatcg agcaggtatt aaaattactc gcgacagttt caaacacgac agtactaccg
     3061 cgctttatga ggtattggaa gatgctgctt ctatatttga ggtacagctt gcgagtaatt
     3121 cgcttgcaaa agcatacttg gcagagcgcg gtgttactga agaaaccatt aaaaaatttc
     3181 ggattggcta tgcaccagat tcgtgggaca cgcttgctcg ccggtacagc ggcataaaac
     3241 aagctgttgc agagcaagcg gggcttttga agccgggtga ccacggactc tacgaccgtt
     3301 ttcgggcgcg gatcatgttt ccgattgggg actcaagcgg cagaattgtt ggattttcag
     3361 ggcgaatgtt tcctgatgtt gagggcggtg caaagtattt aaacagccct gaaacgccgc
     3421 tcttccaaaa atcaaaattg ttgtttgggt ttgataaagc aaagtttgac atcaagcgtc
     3481 gcgggtttgc aattttggtt gaggggcagt ttgatctcat tctctcgcac cagtttggat
     3541 ttactaacac ggttgccacc tcgggcactg cactgggtga agaatcgctc gacggcgcga
     3601 gcagtctgca aacactcgct cgactcacca acaaccttat gcttgcctac gatggtgatg
     3661 aagcagggaa gaaggcaatg gaccgcgccg cacaggtggc gctccggctg gggatgaatc
     3721 caaaagtagc agcactcccc gcagggagcg acccggcaga ctttttgcgc accgaaggca
     3781 gtgctgcgtg gaaagagcag ttgcgtacga gcgagcatgt ggtggtgttc caggcaaaag
     3841 atttactggc aaaacagctc tcgccgcacc agtttgcagc ggggctgcgc gagcgggtgt
     3901 ttccgtacct cgcgctcatc ccctttgcta ttgagcgggt gcagcagctt gagcttgttg
     3961 cgagtgctgc cggactgccg tcagcagcac tccaagaaga tttctctcac tttcttgcat
     4021 ctcgccctaa agaccatact gccagcaccg ctctgcacac agaaccatca aaaaagaaac
     4081 agcagtttga tacaccagca ctgtttcttg ggatactgag ctggcagaca gggcttgctg
     4141 aaccgagtat tgactcggcg ctctaccgtg atcgtgccga ggcgatttca tttgaagaga
     4201 atcgatacac actccctgaa cttactgatg acacactcca cgctgctcgc atgtatgccg
     4261 ctgcatttta ttcaagtctt accaaagaat cgctcgaaac actcttggat gatcagctgc
     4321 agcatatgac cgtttcattt ctccacgggc ttcgcacaca gttcactgtc gagctccatg
     4381 cagctgagcg ggcaggggat gagagtcggg taacaacact tcttagcatc ttgcatcagc
     4441 tttcggtgcg attacaaggg ttgaaagcgc cctaatttgt aaaataacct ttttatgcta
     4501 ttgtaaaaaa attctatgcc acaaaagaaa aaatccaagg ttccacaaaa aaagaaggta
     4561 gtcaacaaaa cctcaaaaaa ggttcaacca aaaaagaagg tggcggcaaa agcccagaaa
     4621 aaagtttctc caaagaagat accagcaaag cctaaagtaa aggcaaaacc aattaccata
     4681 aaacaccccg gcagttcgcc gcggcgcgaa gcaaatactg aaaagaagct gcaagaattg
     4741 cagctgaagg ctgatgaact tattaccaaa ggtaaaaagc gcggcttcat tacctatgac
     4801 gaaattttaa aaacattccc cgatattgag aacaatattt tatttctcga tgagctctac
     4861 gaaaaattta gcactgccgg tgttgatgta cttgagggcg gtaatctgct cgatatcgat
     4921 aatactgaag acctctcaaa atataccaag gatagccaca ccttcagctc ggtgcaaatg
     4981 tatcttaaag atattgggca gtacccgctg atcaatgcag cacaagaaaa agatttagca
     5041 cggcgcatta ttgccggcga catggaagca aagaatttac ttgcccgctc aaaccttcgt
     5101 ttggtggttt caattgctaa gaagtatgca aaccgcacct ctgatttaac acttcttgat
     5161 ctgattcaag agggaaacat cggactcttc aaagcggttg aaaaattcga ctggaccaaa
     5221 ggcttcaagt tttcaaccta cgctacttgg tggattcgcc aatcaattac tcgtgccctt
     5281 gccgaccaat cacgtaccat tcgcatcccc gtgcacatgg ttgaaactat ttcaaaatac
     5341 aaacaggtga tgcgccgcct ggcgcaagat ttgggtcgtg atccgctcgc cgaagaaatc
     5401 gcaaacgaaa tgggtgttga ggttgaaaaa atccatgtga tcgagcagat caaccaagag
     5461 acccgctcgc tcgagcagcc cattggcgac gatgaagaca aatcaacatt cggcgaattt
     5521 atcgccgacg acaaaattct tcgccccgat caagaagctt cacgccggat gctccaagat
     5581 caaatccgcg gagtgcttga agaactctcg ccgaaagaga agcgaattct tgaaatgcgc
     5641 tatgggctca ctgacggcgt gcagcatacc cttgaagaag tggggaagga gttcggagtc
     5701 acccgcgagc gcatccgcca aattgaggca aaggtgcacg aaaagctccg ccagaatgaa
     5761 aaaatcatgc gtctcaagaa ctattttgat taattttttt ct
//
LOCUS       DATIBQ010000024         6318 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U16294
            k141_1903250, whole genome shotgun sequence.
ACCESSION   DATIBQ010000024 DATIBQ010000000
VERSION     DATIBQ010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935789
            Sequence Read Archive: SRR25158331
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 6318)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6318)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:58:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 637
            CDSs (total)                      :: 603
            Genes (coding)                    :: 601
            CDSs (with protein)               :: 601
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6318
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1903250"
                     /isolate="SMAG_U16294"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..776)
                     /locus_tag="VLB02_00745"
     CDS             complement(<1..776)
                     /locus_tag="VLB02_00745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase/squalene oxidase
                     repeat-containing protein"
                     /protein_id="HSE56599.1"
                     /translation="MNTHTAREKALSYVLRVQEKDGGWKSATAADTAGMLQPAVHTTT
                     FAATLILNAIAALPDSLLKQSIVRRGVAYLRTEASTAWTYNYWKRGSSDAKTTVYPDD
                     TDDTFCALAALSQLAPTVVDGTVLAKSVALLTAQEAAVGGPYRTWIIDPKQKRWHNID
                     IGVNSNIAYFLSLHGIELPRLTAYVEHEIEREVYASEFYHNPYLVIYFISRFYTGKNK
                     ETLISFLLKKRTKGGTWGTVLDTALALTTLIRLGYTGSVI"
     gene            complement(792..2264)
                     /locus_tag="VLB02_00750"
     CDS             complement(792..2264)
                     /locus_tag="VLB02_00750"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSE56600.1"
                     /translation="MDTPSLERTNTRKHIFKRGIHFLYRLFIAPRSADEDTRRHEFIL
                     NSILTVSIVLGAILSVFAFADLLLQEHYQGIRVVLIVLITCFYVGLLILSKKGKGIAA
                     SYILVAVYFASTVYGLHQWGVELPLAILSCALMISIASVLISTEFGFIVGSIVSVTVL
                     ILGFLQVYGISTPQTYWKSENLTFNDPIELALMFFIIMVLSWLSNRETEKSLARARAS
                     EYALTLERDNLEQTVEERTRDLKTAQLEKMSQLYRFAEFGRLSSGLFHDLINPLTAIS
                     LQIDTLDHTCHSTIPCIQDSIRRAVHASKRMDHYLQAVRKQIHLEDTKSFFSVNTEAA
                     EAITLLEYQARVNQVRIKFHAHSSIRTYGNPLKLHQAVVNLVSNAIDACAADTPPESN
                     KQHTVHISLILAKDKKEVLLFVSDNGCGIPADSIEHIFDPFFTTKPHEKGTGLGLSTT
                     KRVIEHDFDGSISAVNKKTGGALFIVRFPITKEPKNQPTH"
     gene            complement(2523..3185)
                     /locus_tag="VLB02_00755"
     CDS             complement(2523..3185)
                     /locus_tag="VLB02_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HSE56601.1"
                     /translation="MRLLIVEDDEDIRELLRASFETECFVVDTASDGDRGSYLARTNQ
                     YSAIILDNILPGKNGSRICQELRMLGKTTPILILSVQDEIPQKVHLLHSGADDYVTKP
                     FSFQELLARVRALLRRPAAIEESIFTIEDLVIDCNRQKVMRGAKSIYLTRKEFMLLEY
                     LMKNKSNVLSRGMIMEQVWGADSDPFSNTIEAHILNLRKKINTGKKRKLITTIPGRGY
                     KI"
     gene            3326..4399
                     /locus_tag="VLB02_00760"
     CDS             3326..4399
                     /locus_tag="VLB02_00760"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HSE56602.1"
                     /translation="MVGSVPLSLREFKIVFERALKSFLAERIARYRLLTTDPLVLESV
                     HHAAALITSGGKRLRPYLAYLAYRSYGGRSHSAPVDVQLFLLLELLHGYLLIQDDVVD
                     RGTTRHGILCLQRFIYKKLPYIKIEGDREHSANGQAFIVADLVCAWMYECIARFVPKG
                     NVSALIELFSTILDEVVVGQLLDIGLVGCKTASRAAITKKTELKTARYSAVGPVALGC
                     LLAGKRRGVFTERFGIPFGMAFQVQDDLLDILGNESVTGKCALSDVREGQHTLFTEYI
                     LARGTALQKKQFRSYFGKQFPKKDEPTVRALFITSGAIAHGEKKIQAYVRAAERAVAA
                     SPFTAQYKKEWYGLIETIRKRKQ"
     gene            4446..5195
                     /gene="radC"
                     /locus_tag="VLB02_00765"
     CDS             4446..5195
                     /gene="radC"
                     /locus_tag="VLB02_00765"
                     /inference="COORDINATES: protein motif:HMM:TIGR00608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HSE56603.1"
                     /translation="MQNMYTTIDHTLILADSAVERRYVLKVRDLPKDEKPREKLLRYG
                     PSALSAAELLAVVLSVGTKKEEVLVMANRILKDYGMESMALHTNPKELAADLDIPIGK
                     AMQVISSLELGRRFFRRNANGAPIIRTARDVFEYAADMRTLPKEHLRGIYLNAHYKVI
                     HDETISIGTIDASIIHPREVFKPALEYSAAAVVLVHNHPSGIVEPSEADIAITTQLIS
                     AGKLLGIDLIDHVIVAGDAFKSIPAPYTGGM"
     gene            5200..5493
                     /locus_tag="VLB02_00770"
     CDS             5200..5493
                     /locus_tag="VLB02_00770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56604.1"
                     /translation="MYTITPSFIYFTTAPRTLFLRDSQSERQIDLEAMFRVYVRESYR
                     VLSRELKQFKDLSVPYMPEEEQKLQASLARLIEAKRVFWEERKAARSGIARGY"
     gene            5551..5808
                     /locus_tag="VLB02_00775"
     CDS             5551..5808
                     /locus_tag="VLB02_00775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56605.1"
                     /translation="MDTLIKADIFFFVCTVGFVLLAVIGALVLVQSVLLLQKMNRIAA
                     RIEKNINAVSAETEALIEDVRESALFRFLFGRRRTARSRKT"
     gene            5837..6217
                     /locus_tag="VLB02_00780"
     CDS             5837..6217
                     /locus_tag="VLB02_00780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56606.1"
                     /translation="MKKTTQKKGVSAGTVAAIGAGVAAVGAISYYFFGPHGKKHRKQF
                     TGWMIRMKGEVIEKIEKVSEVTEQVYHKIIDSVAAAYVKSGRASEAQVRMYADELKKQ
                     WKNIAGSAKKHAKKGKPTAKKKST"
BASE COUNT         1642 a         1325 c         1613 g         1738 t
ORIGIN      
        1 tttattacgc tgccggtgta tccgagccga atgagtgtgg taagtgcaag cgcggtatcg
       61 agtacggttc cccaggtacc gcccttggtt ctttttttta ataaaaaaga aatgagtgtt
      121 tccttattct tccctgtata aaatcgggaa ataaaatata taacgaggta cgggttatga
      181 tagaactctg atgcatatac ttctcgttca atctcatgtt caacatatgc agtaaggcgg
      241 ggaagttcaa taccgtggag cgagagaaaa taggcaatgt tgctgttgac tccgatgtcg
      301 atgttgtgcc atcttttttg ttttggatca ataatccagg tgcggtaggg accgccgact
      361 gctgcctctt gcgcggtaag cagtgcgaca ctcttggcaa gcacggtacc gtctaccact
      421 gttggggcga gctgtgaaag tgcagcaagg gcgcagaagg tatcgtcggt atcatcggga
      481 tagacggttg tttttgcatc actcgaaccg cgcttccagt agttgtatgt ccaggcagtg
      541 ctcgcttcag tacgcaggta cgcaactccc cgccgcacaa tgctctgttt tagcagtgaa
      601 tcaggcagtg ctgcaatagc atttagaatc agtgtagcgg caaaggtggt ggtgtgcaca
      661 gctggctgga gcatacccgc tgtatcagcg gcagtagcac ttttccatcc gccgtctttt
      721 tcttgtacgc ggagcacata cgagagtgct ttctcccgtg cagtatgcgt attcatggtg
      781 ataataaaaa attaatgtgt aggctggttt tttggttctt tagtgatagg aaaccgtact
      841 ataaatagcg caccgccggt tttcttattt acagcactga tactgccgtc aaaatcgtgt
      901 tcgatgacac gcttggtggt tgagagtccg agcccggttc ctttttcatg tggtttggtg
      961 gtaaagaagg ggtcgaagat gtgctcgatg ctatcggcgg gaataccgca gccgttatct
     1021 gaaacaaaca ggagcacttc ttttttatct ttcgcgagga tgagggaaat gtgcacggta
     1081 tgttgtttgt tactttcagg cggggtgtct gctgcgcacg catctattgc gtttgatact
     1141 aaatttacga ccgcctggtg gagctttaac ggattgccgt aggtacgaat actgctgtgt
     1201 gcgtggaact tgatccgtac ctggttaacg cgtgcctgat actcaagcag ggttattgct
     1261 tctgctgctt cggtgtttac tgaaaagaat gatttggtat cttctaggtg gatttgtttg
     1321 cgcactgcct gcaggtagtg atccatgcgc ttacttgcat gtactgcccg ccggattgaa
     1381 tcttggatgc aggggatagt agagtgacag gtgtgatcaa gtgtatcaat ctggagcgat
     1441 attgccgtga gtgggtttat taaatcgtgg aaaagccctg aggagaggcg gccaaactcg
     1501 gcgaagcggt acagttgcga cattttttca agctgggcag tttttagatc gcgggttcgt
     1561 tcttcaacgg tttgctcaag gttgtctcgc tcgagcgtga gcgcatactc ggaagcgcgg
     1621 gcgcgggcga gcgatttttc agtttcgcgg ttcgagagcc atgaaagtac catgatgata
     1681 aaaaacatca gcgccagctc aattgggtcg ttgaaggtga ggttttctga tttccagtat
     1741 gtttgcggag tgcttatgcc gtacacttgc aaaaatccga gtatgagcac cgtgacagaa
     1801 acaatactac ccacaataaa tccaaattcg gtactgataa gcacgctcgc aattgatatc
     1861 atcagtgcac acgaaagaat tgctaacggc agttcaacac cccactggtg caaaccgtat
     1921 actgtgctcg caaaatagac tgccactaaa atatatgaag ctgcaattcc ttttcctttt
     1981 tttgaaagga taagaagtcc aacataaaaa caggtaataa gcacgatgag taccacacgt
     2041 attccctgat agtgttcttg tagtagtagg tcggcaaatg caaatacaga gagtatggct
     2101 ccaaggacga tagaaacagt gagaatacta tttaagataa attcatggcg gcgggtatcc
     2161 tcatcggctg agcggggtgc aatgaaaaga cggtacaaaa aatggatacc ccgcttaaaa
     2221 atatgttttc tagtattggt gcgttcaagg gaaggggtat ccattttttt attgcgtggc
     2281 tagctacgct gttttaccat cccacggagt gaatgcagat gcgtgcattg caaacaagct
     2341 cgctggtgcg ctggtgcgcg atgccttgtt cgtgaggagc tctttcattg ttttgttcat
     2401 attgattgag tatcacaaaa aattactaac gggggcaccg cgttccgacc ggacgcgctg
     2461 caccgctctg tagtatagaa agtttacata aaaggaagat aaatttatga taaaacctca
     2521 tgctatattt tataccctcg gccaggaata gtggtaatca actttctttt tttacctgta
     2581 tttatctttt ttctaaggtt gagaatgtgc gcttcaatag tgtttgaaaa tggatcactg
     2641 tcggcacccc acacctgctc catgatcatc ccacgggaga gcacgttgct cttgtttttc
     2701 atcaggtact cgagcagcat aaactctttg cgggtgaggt agatggattt tgccccgcgc
     2761 atcacctttt gccggttgca gtcgatgact aagtcttcaa tggtaaaaat actctcctcg
     2821 atagcagcgg ggcggcgcag gagtgctcga acacgcgcca agagttcttg gaacgaaaac
     2881 ggttttgtaa cgtagtcatc ggcgccgctg tggagcaagt gtaccttttg aggaatctcg
     2941 tcctgtaccg agagtatcag aattggggtt gttttaccaa gcattcgtaa ttcctggcag
     3001 atgcgcgagc cgttctttcc ggggagaatg ttgtctagaa taatagcact gtactggttt
     3061 gttcgggcaa gatacgagcc acggtcgccg tcgcttgcgg tgtcaacaac aaaacactca
     3121 gtttcaaaac tggctctgag gagttccctg atatcttcat catcttcgac gattagtagc
     3181 cgcatagtgg taaccgaggg aataaggtat agcttccttg cccttattct atattgtttt
     3241 cagagaaata cgattgttat gcagtgcata actcgtttat tatttcttca tattttattc
     3301 atgccagaag aatacaatta ccagtatggt gggcagtgtg ccgctatcac tgcgggaatt
     3361 taaaattgta tttgaacgcg cacttaaatc atttcttgcc gagcgcattg ctcgataccg
     3421 gctgctcacc acagatccgc ttgtgttgga atcagtgcac catgctgccg cactgattac
     3481 cagcggcggc aagcggctgc gcccgtacct tgcatacctc gcgtaccgtt catatggcgg
     3541 tagatcacac tcggcgcctg ttgatgtgca gctgtttctc ctgctcgagc tcttgcatgg
     3601 gtatttgctc atacaagatg atgtggttga tcggggtact actcgccatg gaatactgtg
     3661 cctgcagcgg ttcatttata aaaaactgcc ctacataaaa attgaaggcg atcgagagca
     3721 cagcgcgaat ggacaagcgt ttattgttgc cgatcttgtg tgtgcatgga tgtacgagtg
     3781 tattgctcga tttgttccga aaggaaatgt ttctgctctc atagagcttt tttcaaccat
     3841 actcgatgag gttgtggtag ggcagctgct tgatattggg ctcgtgggat gcaaaacagc
     3901 atcgcgtgct gcaatcacta agaaaacaga actgaaaaca gcgcggtata gtgctgttgg
     3961 tccagttgca cttgggtgtt tactcgccgg aaagcgcaga ggggtattca cagaacgttt
     4021 tggtattccg tttggtatgg cgtttcaagt gcaagatgac ctactcgata ttcttggcaa
     4081 tgaatcggta acagggaagt gtgctttaag tgatgttcgt gaagggcagc acaccttgtt
     4141 tactgaatac atacttgctc gtggtactgc gcttcagaag aaacagttcc ggtcatactt
     4201 cggcaagcaa tttccaaaaa aagatgaacc tactgttcga gcgttgttta ttacttcggg
     4261 tgcgattgca cacggggaga aaaaaataca agcatatgtg cgggctgctg agcgggcagt
     4321 tgccgcatca ccttttactg ctcagtataa aaaagagtgg tatggactga ttgaaacaat
     4381 acgaaaacga aaacaatagc ccatgctaca atagcgtact ttatagtaat tttatatctt
     4441 cacttatgca aaacatgtat accaccattg atcacacact tattcttgcc gattcagctg
     4501 tcgagcgccg gtatgtgctg aaggtgcgag atctgcccaa agatgaaaag ccgcgcgaaa
     4561 aactcttgcg gtatggtccg agcgcactct cagctgccga actgttggca gtagtgctca
     4621 gtgttggcac taaaaaagaa gaagtgcttg tgatggcaaa tcgaattttg aaagattacg
     4681 gcatggaaag tatggcactc cacactaacc caaaagagct tgctgccgat ttagatattc
     4741 cgatcggcaa ggcgatgcag gtaattagct cgcttgaact tggtcggcgc ttctttcggc
     4801 gtaatgcgaa cggcgctcct attattcgaa cggcacgcga tgtatttgag tatgctgctg
     4861 atatgcgcac ccttcctaaa gagcacctac ggggcattta cttaaatgct cactacaagg
     4921 ttatccatga cgagacaatc tcaatcggta ctatcgatgc gagtattatt catccccgcg
     4981 aagtgtttaa accagcgctc gagtattcgg ctgcggcagt ggtgctggtg cataatcatc
     5041 cctcgggcat tgttgagccg agtgaagctg acatcgctat taccacacaa ctcattagtg
     5101 ccggaaagtt actcggcatt gatttgatcg atcacgttat tgttgcgggc gatgcattta
     5161 aaagtattcc ggcgccctat acgggaggaa tgtaatagta tgtacacgat tactccctct
     5221 tttatttatt ttactactgc accgcgcact ctctttcttc gcgatagcca aagcgagcga
     5281 cagattgatc ttgaagcaat gtttcgagtg tatgttcgag agtcataccg tgtgctttct
     5341 cgagaattaa aacagttcaa agatttatca gttccgtata tgccagaaga agaacagaaa
     5401 ctgcaggcat cgctcgcacg cttgattgaa gcaaaacggg ttttttggga agagcgcaaa
     5461 gcagctcggt ccggcattgc tcgaggatac tgacgagaaa agtaaattgc tcgagggtgc
     5521 acagcgagcg caggtgctat actaggtggc atggatacgc ttataaaagc agacattttc
     5581 ttcttcgtct gcactgttgg atttgttctt ctggcagtta ttggtgcact ggtgctcgtg
     5641 cagagtgttt tactactgca aaaaatgaat cgaatcgcag cccgcataga aaaaaatatc
     5701 aatgcggtga gtgctgaaac agaagcactt attgaagacg tgcgggagag tgcgcttttt
     5761 cgatttctgt ttggtcgccg cagaacagca cgctctcgta aaacttgagc ggtattacta
     5821 agaaataaaa aaacacatga aaaaaacaac acagaagaag ggagtttctg cgggtaccgt
     5881 ggctgcaatt ggggcggggg tagctgcagt tggcgctatc tcatattact tttttggtcc
     5941 tcacggtaaa aagcatcgca agcaatttac gggctggatg attaggatga agggtgaggt
     6001 tatcgagaaa atcgagaaag tgtctgaggt gactgaacag gtataccata aaatcatcga
     6061 ctccgtagct gctgcctatg taaaatcagg acgggcgagc gaagcgcaag tgcggatgta
     6121 cgcggacgaa ctaaaaaagc agtggaaaaa tattgcaggt tcagcaaaga aacacgcgaa
     6181 gaaagggaaa ccaacagcaa agaaaaagag tacataacta tcttgcatct ttcctctgcc
     6241 tggtctaccg ttaagagagc agtttccttg gtggagattg ctgagtcgaa ttttttaata
     6301 ataagagtgt tatcgata
//
LOCUS       DATIBQ010000025         2606 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U16294
            k141_146925, whole genome shotgun sequence.
ACCESSION   DATIBQ010000025 DATIBQ010000000
VERSION     DATIBQ010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935789
            Sequence Read Archive: SRR25158331
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:58:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 637
            CDSs (total)                      :: 603
            Genes (coding)                    :: 601
            CDSs (with protein)               :: 601
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2606
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146925"
                     /isolate="SMAG_U16294"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            247..543
                     /locus_tag="VLB02_00785"
     CDS             247..543
                     /locus_tag="VLB02_00785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56607.1"
                     /translation="MDPELKGRLKVIEDKLEKNHQLLVRIRRVQKNAGLFRLFYWLVV
                     IGIAFGSFYYLSPYLTQIKKLYSDASDTFNFSTPEVKSVKNLWNQLNGNGENKE"
     gene            631..703
                     /locus_tag="VLB02_00790"
     tRNA            631..703
                     /locus_tag="VLB02_00790"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:664..666,aa:Phe,seq:gaa)
     gene            complement(934..1446)
                     /locus_tag="VLB02_00795"
     CDS             complement(934..1446)
                     /locus_tag="VLB02_00795"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HSE56608.1"
                     /translation="MTIPPLKKKSFFERLAGNLGFDDSDDFEDSESVKIERKNNPLSH
                     VSHTPQKEEQHIAHVEAAAELAVDVYQTANDIVIQAIIAGTSPENLSINITRDSVTVK
                     GKREENRTVVRENYITKELFWGSFIRTVQLPAEVEIDAAEAICKNGMLLIKLPKLDKY
                     RQTSIKVKTI"
     gene            complement(1443..2156)
                     /locus_tag="VLB02_00800"
     CDS             complement(1443..2156)
                     /locus_tag="VLB02_00800"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family glutamic-type intramembrane
                     protease"
                     /protein_id="HSE56609.1"
                     /translation="MAGPVTIQTVLYVALTGLIPSVVWLIFWLRRDRTRPEPFGLLLI
                     CFLLGAVGVVLAIPLQKFVQSIVTESQTQIILWATIEEVLKFLPVALIALRSKYNDEA
                     IDPAMYMIVSALGFATLENIAYALQPAQALHADAALLNGSLRFLGATLLHVVSSGFVG
                     IVIGLAPRLGKFFFSLFGLAAAIFLHSTFNFFIMKNASASFVQVYGYLWVAAIITLVT
                     LEKLRRIPPYTSVPAPTTL"
     gene            complement(2141..>2606)
                     /locus_tag="VLB02_00805"
     CDS             complement(2141..>2606)
                     /locus_tag="VLB02_00805"
                     /inference="COORDINATES: protein motif:HMM:NF019869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HSE56610.1"
                     /translation="TAPGTDSKISEDKIKGYKHFANKLWNITRFVLTNCSATESAYTD
                     EDHVLLNEWQQVAERVTEDIEQYRLHLATEKLYQYVWTRFADEILEESKLIFNGTNSE
                     ARISRQKLLLELLAGNIKLLHPFMPFVTEALWELLPLQNKRPFVMIEPWPVR"
BASE COUNT          713 a          513 c          649 g          731 t
ORIGIN      
        1 attttacgaa gagcgggaat tgtagaaact aaacggagtg gtaaatttat ttgctattct
       61 atttgttacg atgaaataga aagaataaat aacattgctg ctgagttagt gaagtagtaa
      121 ccaaataaat atgatgtaca aaaggtcttg aacttcaaga cctttttctt ttttagcaaa
      181 atattagtgt ctgaatttta gattcttttt cttgaaaaaa gcctgatttc tgctaggatg
      241 taaggtatgg atccggagct gaaggggcgg ttaaaggtta ttgaagataa gcttgagaag
      301 aaccaccagc tcttggtccg gattcgccgg gtgcaaaaaa atgccggatt gttccgtctg
      361 ttttactggc ttgtggtgat aggtattgca tttgggtcat tttattacct gagcccgtac
      421 cttacgcaga ttaaaaagct ctacagtgat gcttcagata cctttaattt ctcaactcca
      481 gaagttaaga gcgtaaagaa tttgtggaat cagttgaacg gaaatggtga aaataaggaa
      541 taaattttag aatacgtttg ctcggggaag aaacttgccc gagcggcgtt cggagaaaat
      601 aaaaatatat ttttatttta ctcctcactt gctcgggtag ctcagttggt agagcgctcc
      661 cctgaagagg gagatgtcac cagttcaagt ctggtcccga gcacaattag aaatgccctg
      721 atattttttc agggcatttc taattgcttg gattgcagac ttgaaccaga agggggtcgg
      781 ggaaactggg tgtttccccg tggaggaagg cagcgagtct tcgagcgttg gaaaaccctg
      841 ggtttccaag agagtgaaat tctggtcccg agcacaataa gaaaaacctg gatttttttc
      901 aggtttttct tattgcttgg attacaggtt attttatatc gtcttcactt taatagacgt
      961 ctgccggtac ttatcaagct tagggagttt tatgagcaac ataccattct tgcagattgc
     1021 ttcagcggcg tctatttcaa cttcggcggg gagctgcaca gtgcggatga acgagcccca
     1081 aaagagctct ttagtgatgt agttttcgcg aacgacagtg cggttttctt cacgctttcc
     1141 ttttacggtt actgaatcgc gggtaatgtt gatggaaagg ttttcgggcg aggtaccggc
     1201 aataatcgct tgaataacaa tgtcgttggc agtttggtat acatcaacag caagttcggc
     1261 tgctgcttca acatgtgcta tgtgctgttc ttctttttgt ggagtgtgcg aaacgtggga
     1321 aaggggatta tttttccgtt caatttttac tgattctgaa tcttcaaaat cgtcgctatc
     1381 atcaaatccg agattgccgg cgagccgttc aaaaaaactc tttttcttta ggggtggtat
     1441 tgtcatagtg ttgtaggggc aggaacagaa gtatacggcg gaatgcggcg cagtttttca
     1501 agggtgacaa gggtaataat tgctgcgacc cacagatagc cgtacacctg tacaaaactt
     1561 gcagatgcgt ttttcattat aaaaaaatta aaggtactat gcaagaagat tgcagcagcg
     1621 agcccgaaga gtgaaaagaa aaacttcccc agtcgcggcg caagcccgat gacaataccc
     1681 acaaatccgc ttgaaacaac gtgcagcaaa gttgccccta aaaaacgcag cgaaccattc
     1741 aacaatgcgg catcggcatg aagcgcctgc gctggctgca atgcgtaggc aatattttca
     1801 agcgtggcaa atccgagcgc cgagacgatc atgtacattg cagggtcgat tgcctcgtcg
     1861 ttgtacttgc tccgcagtgc aatcagggca acgggcagaa atttaagcac ttcctctatt
     1921 gttgcccata aaattatttg agtttggctc tcggtaacaa tactctgtac aaatttttgg
     1981 agtgggatag caagtactac accaaccgca ccgagtaaaa aacagatcag gaggagccca
     2041 aagggttctg ggcgggtgcg gtcgcggcgg agccaaaaaa tgagccacac tacagaggga
     2101 atcaagccgg taagcgcaac atagagcact gtttgtatgg ttaccggacc ggccatggtt
     2161 caatcataac aaatggacgc ttgttctgca gcggcaagag ctcccagagt gcctcggtaa
     2221 caaacggcat gaaggggtga aggagtttaa tgttgccggc gaggagttcg agtaatagtt
     2281 tttgccgtga aatacgagcc tcgctgttag ttccattaaa aataagctta ctctcttcca
     2341 aaatttcatc cgcaaaacga gtccacacgt actggtagag tttttcggta gccaggtgga
     2401 ggcggtactg ctcaatatct tcagttactc gctctgcgac ctgctgccat tcattgagga
     2461 gtacgtggtc ttcatcggta tatgcacttt ctgttgctga gcagttagta agtacaaagc
     2521 gggtaatgtt ccagagcttg ttcgcaaagt gcttgtagcc cttgatttta tcttctgaaa
     2581 ttttactatc ggtacctggt gcagtg
//
LOCUS       DATIBQ010000026         4499 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U16294
            k141_814215, whole genome shotgun sequence.
ACCESSION   DATIBQ010000026 DATIBQ010000000
VERSION     DATIBQ010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935789
            Sequence Read Archive: SRR25158331
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 4499)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4499)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:58:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 637
            CDSs (total)                      :: 603
            Genes (coding)                    :: 601
            CDSs (with protein)               :: 601
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4499
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_814215"
                     /isolate="SMAG_U16294"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..194
                     /gene="rplU"
                     /locus_tag="VLB02_00810"
     CDS             <1..194
                     /gene="rplU"
                     /locus_tag="VLB02_00810"
                     /inference="COORDINATES: protein motif:HMM:TIGR00061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HSE56611.1"
                     /translation="MVESGAATTIGTPFIAGTTVTGTVEMVGKKPTVTVIKYKAKSRY
                     FKKNGHRQPYCKVKITAIK"
     gene            complement(235..840)
                     /locus_tag="VLB02_00815"
     CDS             complement(235..840)
                     /locus_tag="VLB02_00815"
                     /inference="COORDINATES: protein motif:HMM:NF025044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HSE56612.1"
                     /translation="MDSRLQKLAQLFLKFPGIGERQARRFAFFILNQQQGYIQELTET
                     IHTARSGAKQCPQCFRLHESASIRCAICSSPNRDQSRLLIVEKQADIEAVEQTSYNGL
                     FFIFGGLIPIVEKKTMAKTRITELEARITTDVEAGVLKEVILAFPLTANGDHTEIYLR
                     ELLQKKFPTLSVTSLGRGLSMGSELEYIDPRTLEISIAKRE"
     gene            complement(843..2222)
                     /gene="dnaB"
                     /locus_tag="VLB02_00820"
     CDS             complement(843..2222)
                     /gene="dnaB"
                     /locus_tag="VLB02_00820"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015049249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HSE56613.1"
                     /translation="MSKPQNLATLRIPPHDLDAEQAVLGALMLRPSAIHEIVDLLAPD
                     MFYARKHALVYQTIMELSIKNEPIDVLSVSNKLKEKKLLKDIGSNTYLNELMTTVPST
                     INVRHYADIVYKKYILRSLIDASEHISSLSYDEGSDDVATILDEAEKTIFKVSSSMNV
                     KGAFTSIKQSLIETWETIEHLHENKDELRGVPTGFPDLDNILAGLQKSDLIILAARPS
                     VGKTTFALDIARHAALTGIPVGIFSLEMSNQQLVQRMLSAESRVDAWSIRTGHGLNSD
                     HFTALQEAASRLGKAPIYIDDQAGNSIVKMRAVARRLKSEHGLGLIVVDYLQLMTTQK
                     NYDNMVNQVTEISRSLKQLARELDVPVLALSQLSRAVEARGGKPRLSDLRDSGSIEQD
                     ADVVMFIHREDRYKDQSERDNITEIMIEKHRNGAVGSIQLYFDLKTTTLVSIDKAHIP
                     VPAKSLDDF"
     gene            2270..3274
                     /gene="trpS"
                     /locus_tag="VLB02_00825"
     CDS             2270..3274
                     /gene="trpS"
                     /locus_tag="VLB02_00825"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HSE56614.1"
                     /translation="MKKAFISDTIVPMESTKRRVLSGVQPSGRLHIGNYFGAIKQFVA
                     LQETHDCTFMIADLHALNTVQDEVRMRQQIFDIVVDYLALGIDPKKCIIFKQSAVLAH
                     PAMAWIFDTLTTMPYLMRAHAFKDAEAKNKEISVGVFNYPLLMASDILLYSPDIVPVG
                     ADQKQHVEIARDAAEKFNRIYKPVFKIPEAYIMSETAVVPGTDGRKMSKSYNNTIPLF
                     ATDEEITKAVMSIPTDSKGKDEEKNPDDIVLYQIHKLFDASDELRARYTSGLGYKDAK
                     ELLIKDLIAFIAPLRERRAKYEQDPKLVQEILLKGAMCANEVAMRKLTDVYEAVGLTD
                     "
     gene            3289..>4499
                     /locus_tag="VLB02_00830"
     CDS             3289..>4499
                     /locus_tag="VLB02_00830"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSE56615.1"
                     /translation="MKKQKSFIKKIIQFFRPFWKYFAITMFLIVVVQAFAAFTPYLFG
                     KGVDAVIKGNVRLTFIFLIVSFLITILQQQVIWYLKEYIDIKKLDINIDRALSIISLK
                     RMFQFSIGQHINEHSGVRQTIVNKGQNSLNGLVNNFIYTILQTGLQVLVILVILILFD
                     WRVGATAFAFMLLHILISNRRNHVYFPKIDEVRKKNQAQSKLQSELFRNATLVIAEAQ
                     EDKTVEDFSRSASKATDFTVTTWLKYIRSYYSQKFILIIGRYATLAVGIYFIFIGDHS
                     VGMFVTFYSWTGSIFDNINQIMNSQRQIMFQIVEIKKYFELLDIIPDVNPNIGGKTLE
                     NLAGKIEFEHVSFAYPYRKSAKEEEEESANEKDQEHAIQNINLTIPAGAKVGFVGVSG
                     SGKSTIVNLMRR"
BASE COUNT         1281 a          934 c         1024 g         1260 t
ORIGIN      
        1 taatggtaga gagcggtgct gcaaccacta tcggcacgcc gtttattgcc ggtacaacag
       61 taaccggaac agtagaaatg gtaggaaaaa aaccaaccgt aaccgttatt aagtacaagg
      121 caaagagccg ctactttaag aaaaatggtc accgccagcc gtattgtaaa gtaaaaatta
      181 ccgcgattaa gtaaatttat ttgaaaaaac cgagaaataa tttctcggtt tttttcattc
      241 tcgttttgcg atcgaaattt caagggttcg gggatcaatg tattcaagtt cgctgcccat
      301 agagagcccg cggccgagac tggtaactga aagggtaggg aattttttct gcaacaattc
      361 gcgcaggtat atttcagtat ggtcaccgtt ggcagtgagc gggaaggcga gaatcacttc
      421 ttttaatact cccgcttcaa cgtctgtagt aatacgcgct tcaagctctg taattctcgt
      481 ttttgccatc gtcttttttt caacaatagg gatgagcccg ccaaaaataa agaagagtcc
      541 attgtacgaa gtttgctcaa ctgcttctat atctgcctgt ttttcgacaa tcaacagccg
      601 agactgatcg cggtttggac tgctacaaat agcacagcgt atgctagcgc tctcatgcag
      661 ccgaaagcac tgcgggcact gttttgcacc gctccgtgcg gtgtggatgg tttcagtcag
      721 ttcttgaatg tacccctgct gctggttgag aataaaaaaa gcaaaccgcc gcgcctgccg
      781 ctcaccaatg ccggggaatt taagaaacag ctgggctaat ttttgtaatc ggctatccat
      841 gcttaaaaat catcgagcga ttttgctggt accggaatgt gcgctttgtc gatagaaaca
      901 agggtggtag ttttgaggtc gaagtagagc tggatagaac caacggcgcc gtttcggtgc
      961 ttttcaatca taatttcggt aatgttatcg cgctcggatt gatctttgta gcggtcttca
     1021 cggtgaatga acatcaccac atcggcgtct tgttcgatag agccggaatc gcgcaaatca
     1081 gagagacgag gcttgccgcc gcgagcttca acggcacgcg agagctgtga gagcgccagt
     1141 acgggcacat cgagctcgcg ggcgagctgc ttgagcgagc gtgaaatttc ggttacctgg
     1201 tttaccatgt tgtcgtaatt tttttgtgtg gtcatcagct gtaggtagtc aaccacgatt
     1261 aacccaagcc cgtgctcgct cttcaatcgg cgggcaacgg cgcgcatttt tacaatcgaa
     1321 ttgcctgcct gatcgtcaat ataaatgggc gctttaccta aacgcgaagc tgcttcttgt
     1381 agggcagtga agtggtcgct atttaagccg tgccccgtac gaatactcca ggcatcaact
     1441 cggctctctg ccgagagcat gcgctgcacc agttgctgat tgctcatttc gagcgaaaaa
     1501 atacctaccg gaattccggt cagtgctgcg tgtcgggcaa tatcgagcgc aaaggtagtt
     1561 ttaccaactg atggtcgtgc tgccaaaata ataagatcag atttctgcaa gccagccagt
     1621 atgttatcga ggtcagggaa gccggtcggt acgccgcgga gctcgtcttt attttcgtgc
     1681 aagtgctcaa ttgtttccca tgtttcaatc agggactgct tgatagaggt aaaggcaccc
     1741 ttcacattca ttgaacttga tactttaaaa attgtttttt ctgcttcatc taaaatggta
     1801 gcaacatcat cgctcccctc atcgtacgag agggatgaaa tatgctcgct ggcatcaata
     1861 agcgaacgca aaatgtattt tttgtagaca atgtcggcat agtggcgaac gtttatcgtt
     1921 gagggcacgg ttgtcatcaa ctcgtttaaa taggtgttgc tgccgatatc tttgaggagc
     1981 tttttttctt tgagtttgtt tgaaaccgag agcacatcaa taggctcgtt ttttatcgag
     2041 agctccataa tcgtttggta taccagtgca tgcttgcgcg cgtagaacat gtcgggtgcc
     2101 aacaagtcga caatttcatg aattgcgctc gggcggagca tcaatgcacc gagcactgcc
     2161 tgttcagcat caaggtcgtg cggcggaatc cgcagtgttg ccaagttttg aggtttagac
     2221 atctgtctat tctatcaccc aagcgatgca cgtgcgctac agcgggcaca tgaaaaaagc
     2281 ctttatttct gatacaattg tccccatgga atccacaaag aggcgggtat tatcgggcgt
     2341 gcagccgagc ggcaggctcc acatcggcaa ctactttggg gcaatcaagc agtttgttgc
     2401 actgcaagag acacacgact gcaccttcat gatcgccgat ctccatgcgc tcaacaccgt
     2461 acaagacgaa gtacggatgc ggcagcaaat atttgatatt gtagtcgact acctcgcttt
     2521 aggaattgat cctaaaaaat gcattatttt caagcaatcg gcagtgctcg cacatccagc
     2581 catggcttgg atttttgata ctttaacgac gatgccgtac ctgatgcggg cacacgcatt
     2641 taaagatgcc gaggcaaaga ataaagaaat tagtgttggg gtgtttaact acccgctcct
     2701 tatggcttca gatattttac tctacagccc cgatattgtt ccggtaggtg ccgatcaaaa
     2761 acagcatgtt gaaattgccc gcgatgcagc tgaaaaattt aaccgtattt ataaaccggt
     2821 atttaaaatt cctgaagcgt acattatgag cgaaacagca gtagtgccag gaactgacgg
     2881 cagaaaaatg tcaaaaagct acaacaacac catcccgcta tttgcgactg atgaagaaat
     2941 tactaaagca gtaatgagta ttccaaccga ctcaaaaggg aaggacgaag agaaaaatcc
     3001 tgatgatatt gtactgtacc agattcacaa actctttgat gcttcagacg agctacgagc
     3061 gcgctacacc tcgggtttgg gctacaagga tgcaaaagag ctgctcatca aagatttaat
     3121 tgcctttatt gcaccactgc gcgaacgccg agcgaagtat gaacaggatc caaaactggt
     3181 gcaagaaatt ctccttaaag gagcaatgtg tgccaatgaa gtagcaatgc gaaaactgac
     3241 ggatgtatac gaggcggtgg ggctaacgga ttagttaact ttttacagat gaaaaagcaa
     3301 aagtctttta ttaaaaaaat aattcaattt tttagaccat tttggaagta ctttgccatt
     3361 actatgtttc taattgtagt ggtccaggct tttgctgctt ttactccata cctgtttggt
     3421 aaaggtgttg atgctgttat aaaagggaat gttcgactta cattcatttt tttgattgtt
     3481 tcttttttaa tcaccattct tcagcagcaa gttatttggt acttaaagga atatattgat
     3541 attaaaaaat tggatatcaa tatcgataga gctctctcta tcatttcttt gaaaagaatg
     3601 tttcaatttt ccattggaca gcacatcaat gaacattctg gtgtccgtca aacaattgtt
     3661 aataaggggc aaaattcatt aaatggtttg gtaaataact ttatttacac catactccaa
     3721 acaggactcc aggttctagt tattttggtg attttaatat tatttgattg gcgggtgggg
     3781 gcaacagcat ttgcatttat gttgctccat atactgatct caaatagaag aaaccatgtt
     3841 tactttccga aaattgatga agttcgaaag aaaaatcaag ctcaatcgaa actacaatct
     3901 gaattattta gaaatgcaac attggttatt gccgaggcgc aagaagataa aacagttgaa
     3961 gattttagcc gctctgcgag caaggcaact gatttcactg ttactacttg gctcaaatat
     4021 atcaggtcgt actacagcca aaaatttatc ctcattatcg gaaggtatgc cacgttagct
     4081 gtgggaattt attttatttt tatcggagat cattcagttg gaatgtttgt tactttctat
     4141 tcttggaccg gctcaatctt tgacaacatt aaccagatca tgaattccca acgccaaatt
     4201 atgtttcaaa tcgtggagat taaaaaatat tttgaactgc ttgatattat tccggatgta
     4261 aatccaaata ttggaggaaa aaccttagaa aatttggctg ggaaaattga atttgagcat
     4321 gtcagttttg cgtatccgta ccgcaaatct gcaaaagaag aggaggaaga aagtgctaat
     4381 gaaaaagatc aagagcatgc aattcagaat attaacctca ctattccagc aggagcaaaa
     4441 gtaggctttg tgggagtctc aggatctgga aaatcaacca tagtcaattt aatgcgccg
//
LOCUS       DATIBQ010000027         3202 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U16294
            k141_1109571, whole genome shotgun sequence.
ACCESSION   DATIBQ010000027 DATIBQ010000000
VERSION     DATIBQ010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935789
            Sequence Read Archive: SRR25158331
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:58:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 637
            CDSs (total)                      :: 603
            Genes (coding)                    :: 601
            CDSs (with protein)               :: 601
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3202
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1109571"
                     /isolate="SMAG_U16294"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            85..1158
                     /locus_tag="VLB02_00835"
     CDS             85..1158
                     /locus_tag="VLB02_00835"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HSE56616.1"
                     /translation="MRRPLIFLGLLFLATGFMYQITGPWQFSTIKAAVLESVVAKTVP
                     KTETLTIAWVGDMVPPESEAYAAGALTTVATPLTDADITLGNLEGTFATSSTGSKCGT
                     GSSNCHAFRGTPTFASILKTTGFDAVSLANNHSLDFGEKGLGETETVLAKAALPFVSS
                     RQPVTYIAKNGFTIGLVGISSTPPQETITDYDFIQKKVSEAKAKSDLVLVLFHGGAEG
                     SDKLLVPNGYEYLGNENRGFVRRAAQVAIDAGADGVLGSGPHVLRGIELYHGKPIAYS
                     LGNFVGSEKLITKGVLATSGILKITFAKDGTFIDGEVVPVTLDKAGVPHLDVAQAALA
                     LVAKLSQQNFGETGALFTGNLFK"
     gene            complement(1290..2969)
                     /gene="groL"
                     /locus_tag="VLB02_00840"
     CDS             complement(1290..2969)
                     /gene="groL"
                     /locus_tag="VLB02_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020861279.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HSE56617.1"
                     /translation="MSKKIVQGTEAQALLKRGIDAVANAVKITLGPRGRNVVFDKGYG
                     VPTVTNDGVSIAKEITLANKFENMGAEIVKEVASKTNDAAGDGTTTSVVLTQAIITEG
                     LKRTGMGANGIVVRQGIEAAAADVVAALKVMAKPIKNKEEIAAVATISAESEEIGGII
                     AETIEKVGKDGVVTVEESQAFGVDSELVEGMEFDKGYISHYMITNQERMEASYSNVPV
                     LVTDKKISAVKDILPLLEKLAQTGKKEIVIIADDVDGEALATFLVNKIRGIFNILAIK
                     APGYGDRKKEQLADIAVVLGAKVISEDIGQTLESAELAMLGRASKVIATKDKTTIVDG
                     KGKKEEIKARVQTLKAQLQNTTMKYDREHLEKRIAKLASGVAVIKVGAATETEMKYLK
                     LKIEDAVNATKAAIEEGIVPGGGVALAKVAQKIEKKMKASAQYKKSHESKRESEFVIG
                     YLALVSALTAPLRNIAFNAGHEDPALIVAMVQEAAGEHMGYDASADSLEGTVVAVDLM
                     KKGIIDPVKVTRTALQNAASAAGILLTTDVAIADEPEKEKAIQDGAPDMGY"
BASE COUNT          801 a          833 c          668 g          900 t
ORIGIN      
        1 acaaaattcg catcaccgtg gcaaacaccg acattcacaa gcgattgatt gattataaga
       61 tggctgcacc cctctcaacc tagtatgcgc cgcccgctca tttttttagg cttgctgttt
      121 ctggcaacag ggtttatgta ccagataact ggaccatggc agttttctac tatcaaagcc
      181 gctgtacttg aaagtgtagt ggcaaaaaca gtaccaaaaa ctgaaacact caccattgcc
      241 tgggtgggcg atatggtacc gcccgagagc gaagcatatg cagcaggagc tcttaccact
      301 gttgcgacac cacttactga tgccgatata acactgggca acctagaagg aaccttcgcc
      361 acctcaagca caggatcaaa atgtggaaca ggcagcagta actgccacgc attcaggggc
      421 acacccacat ttgcttccat cctaaaaaca accggttttg atgcggtttc gcttgctaat
      481 aaccactcgc tcgattttgg cgagaaaggg ttaggagaaa ccgaaacagt acttgcaaaa
      541 gcggcactcc cgtttgtctc gagccgccaa ccagtaacct atattgcaaa aaatggattt
      601 acgattgggc tcgttgggat ttcaagtaca ccgccacaag aaacaattac cgactacgat
      661 tttatacaaa aaaaagtttc agaagcaaaa gcaaagagcg acctggtgct cgtactcttc
      721 cacggtggtg ctgaaggttc agataaatta ctggtgccaa atgggtatga atacttagga
      781 aacgaaaacc gcgggttcgt acgccgcgct gcacaagttg ccattgatgc gggtgctgac
      841 ggcgtgctcg gctcaggtcc gcacgtgctg cgtggcattg agctttacca cggaaaacca
      901 attgcctata gcttggggaa ttttgtcggt tctgaaaaac tgattaccaa aggggtactc
      961 gctacctcgg gcatcttgaa aattacgttt gcaaaagacg gcactttcat agatggcgaa
     1021 gtcgttccag taacgctcga caaagcggga gtaccacacc tagatgttgc acaagctgcc
     1081 cttgcgcttg ttgcaaaact ctcacagcaa aactttggag aaactggtgc cctatttact
     1141 ggtaatttgt tcaagtaaaa aagccccgta gaaacggggc tgaccaaaac caaactcgac
     1201 tgcttataat cggttgggtt acattataac cacaacttaa aagaaaaagc cagactcata
     1261 gagtctggct ttttcttttt taatttctgc tagtagccca tatcgggagc accatcttga
     1321 atagcttttt ctttttctgg ctcatcggca attgctacat cagttgtcag aaggattcct
     1381 gcagccgaag cagcattttg gagggcagtg cgggttactt ttactgggtc aataatcccc
     1441 ttcttcatca aatctaccgc taccaccgta ccctcgagtg aatctgccga agcatcgtaa
     1501 cccatatgct cacctgctgc ctcttgtacc atcgctacaa tcagcgccgg gtcctcgtgc
     1561 cctgcgttga aggcaatatt gcgcagcggt gccgtgagcg ccgaaacaag tgcgaggtag
     1621 ccgatcacaa actcgctctc gcgctttgat tcgtggcttt ttttatactg tgcactcgcc
     1681 ttcatctttt tttcaatttt ttgtgccact tttgcaagcg ctacgccgcc gcccggcaca
     1741 attccttctt caatcgctgc tttggttgca tttactgcat cttcaatttt tagcttcaga
     1801 tacttcatct cggtttcagt tgctgcaccc actttaataa ccgctacccc tgatgcaagt
     1861 tttgcaatcc gcttttctaa gtgctcgcga tcgtacttca tggtggtatt ctgaagctgc
     1921 gctttaaggg tctgcacacg ggcttttatt tcttctttct tgcccttgcc gtcaacaatg
     1981 gttgttttgt ctttggtggc aattacctta ctagcacgcc cgagcatcgc aagttcagca
     2041 ctttcgagcg tctgcccaat atcttcactg attacttttg ctcccagtac cacagcaatg
     2101 tcggcaagct gctctttttt tcgatcaccg tagccaggcg ctttgattgc caaaatgttg
     2161 aagataccgc gaattttgtt taccaaaaaa gtggcaagtg cttcaccatc aacatcatcg
     2221 gcaataatca caatttcctt tttacctgtt tgtgcaagct tttccagcag cggcaaaata
     2281 tctttgactg ctgaaatctt tttatcagtt accaacaccg gcacattcga gtagctcgct
     2341 tccatgcgtt cttgattggt aatcatgtag tgtgaaatgt agcccttgtc gaattccatt
     2401 ccttctacga gttctgaatc aaccccaaaa gcctgcgatt cttcaaccgt caccacaccg
     2461 tctttgccca ctttctcaat tgtttcagca ataattccgc caatttcttc actctcggct
     2521 gaaatagtag caactgcagc tatttcttct ttattcttaa tcggctttgc catcacttta
     2581 agcgccgcta ctacatcggc agctgcggcc tcaatcccct ggcgcacgac aataccgttt
     2641 gcacccatgc cggtgcgttt gagtccttca gtaataattg cctgggtgag caccaccgaa
     2701 gtggtcgtgc catcgccggc tgcatcgttt gtttttgagg caacttcttt aacaatttca
     2761 gcacccatgt tctcaaactt gtttgcaagc gtaatctctt ttgcaattga tacaccatcg
     2821 ttggttactg ttggcacgcc gtagccttta tcaaacacga cgttgcgccc gcggggtcca
     2881 agcgtgattt ttaccgcatt cgctactgca tcaatgccgc gcttcaaaag cgcctgtgct
     2941 tctgttcctt gtacaatttt tttagacata tcttcagata ataaacaata atcacattcc
     3001 aataatcaat aaatacttga gcgaagcttg taattcgagc agtgtctcgc gcagccggcg
     3061 taaggcgagc ggagcaattt gccacgaggc gaatatgcgt gcactgcgag agttataagc
     3121 aagcgaatac acttctttta ctctacttca caatcgccaa cacctgctct tctcgaagaa
     3181 tagagtaatt ctctccgtca at
//
LOCUS       DATIBQ010000028         3443 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U16294
            k141_1734618, whole genome shotgun sequence.
ACCESSION   DATIBQ010000028 DATIBQ010000000
VERSION     DATIBQ010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935789
            Sequence Read Archive: SRR25158331
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3443)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3443)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:58:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 637
            CDSs (total)                      :: 603
            Genes (coding)                    :: 601
            CDSs (with protein)               :: 601
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3443
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1734618"
                     /isolate="SMAG_U16294"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1400
                     /locus_tag="VLB02_00845"
     CDS             <1..1400
                     /locus_tag="VLB02_00845"
                     /inference="COORDINATES: protein motif:HMM:NF014044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HSE56618.1"
                     /translation="LLSVDELATLFHFPVGLIAAPELKESSATTAAAPMSMQPSGVRL
                     GTNTYRGETRDVYLAKEDRVRHCYVIGQTGTGKTTILKNMAIQDIQNGNGVCFIDPHG
                     SDLNDILANIPPERLDDVIYFDPAYTARPMGLNMLEYDANFPEQKIFVINELLAIFNK
                     LFDMKVAGGPAFEQYFRNSAGLVMEDPESGSTLLEIGRVLSDKAFRDLKLSKCKNPII
                     KQFWENAEKTSGEQSLANFVPYITNKFDVFISNDVMRPVIAQQHSVLNFRDIMDNKKI
                     LLVNLSKGRLGDINSHLIGLVLVGKLTMAALSRVDKLGKEVLNDFYLYIDEFQNVTTD
                     SIATILSEARKYRLALTVAHQYVEQLEENIKNAVFGNVGSMIIYRVSPENAALFEKQL
                     APVFTPADILKLENFNAYAKILVNGVPEKPFNLRIPPPPKGNLEVTEKVKELSYLKYG
                     RPREEVESEIMARYK"
     gene            1415..1645
                     /locus_tag="VLB02_00850"
     CDS             1415..1645
                     /locus_tag="VLB02_00850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56619.1"
                     /translation="MENMEKPVPDFEDYRNLSEDATAQQRINDIVERATERGSPLTKE
                     ERDEINQLKSHREVDELIEKAIRNRPDGTEEF"
     gene            2031..2303
                     /locus_tag="VLB02_00855"
     CDS             2031..2303
                     /locus_tag="VLB02_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016339130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HSE56620.1"
                     /translation="MNKQSLVEFLHSKFGGTKVQAEEAVEGIIAEITKTLKKGEDVSI
                     AGLGIFSVKTRAARMARNPKTGEQVKVAATRVARFRPAKALKDSVK"
     gene            2402..2755
                     /locus_tag="VLB02_00860"
     CDS             2402..2755
                     /locus_tag="VLB02_00860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56621.1"
                     /translation="MHKNTILFLTNFEKREFSGFEAIAKKVLLQQQNTCVSFVNELKT
                     APETDELVKKKKPNLVIFKDIATELVQSTIARIKKTLGNSVEVEFLTLGKKILPNVEM
                     VFAVEDIGKRLVVCN"
     gene            complement(2968..3038)
                     /locus_tag="VLB02_00865"
     tRNA            complement(2968..3038)
                     /locus_tag="VLB02_00865"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3004..3006),aa:Gly,seq:tcc)
     gene            complement(3107..>3443)
                     /locus_tag="VLB02_00870"
     CDS             complement(3107..>3443)
                     /locus_tag="VLB02_00870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56622.1"
                     /translation="SNDCLVVTVPQAGKVVSAPFIVSGFIKQRCAWMISDGNTAAGTV
                     QLANTAGATLGVPSYVRVHSITNPPRYPLFFTAQINAPVTPVSGAAMLLFSDNAPVPN
                     TKLYQVTLK"
BASE COUNT         1028 a          800 c          761 g          854 t
ORIGIN      
        1 tcttgctctc agtcgacgag cttgcaacac tgttccactt ccctgttggg ctcattgctg
       61 caccagaact caaagagtct tcagcgacaa cagcagcagc gccaatgagc atgcagccaa
      121 gcggcgtgcg cctgggcacc aacacttacc gcggcgaaac acgcgacgtg tatttggcaa
      181 aagaagatcg cgtgcgccac tgctacgtga ttggacaaac cggaaccggt aaaacaacta
      241 ttttaaaaaa catggcaatc caggatattc agaacggcaa cggtgtgtgc ttcatcgatc
      301 cgcacggctc tgacctaaac gacattttgg caaacattcc acccgagcgc ttggacgacg
      361 taatctactt cgaccctgcg tacactgccc gcccgatggg gcttaacatg ctggaatacg
      421 atgcgaattt tcctgagcag aaaatttttg tcatcaacga gctcctcgct atttttaata
      481 aactctttga tatgaaagtt gccggcggtc cggcgttcga gcagtatttc cgcaactcag
      541 cagggctggt gatggaagac cccgagagcg gctcaacctt actagaaatt ggacgggtac
      601 tttccgacaa agcgttccgc gacctgaagc tttcaaagtg caaaaatccg atcatcaagc
      661 aattttggga gaatgctgaa aaaacgagcg gcgaacaatc actcgctaac ttcgttccct
      721 acatcaccaa caagttcgat gtcttcattt caaacgatgt gatgcgtccg gtaatcgccc
      781 agcagcactc ggtgctcaat ttccgtgaca tcatggataa caaaaaaatc ctgctggtaa
      841 atctttcaaa agggcgcttg ggcgacatta actcgcacct catcgggctt gtgctcgtgg
      901 gcaagctcac catggcagcg ctctcgcgtg tcgacaagct cggcaaagag gtgctcaacg
      961 acttctacct ctacattgac gaattccaga atgttacgac cgattccatt gcaactattc
     1021 tttcagaagc gcgtaagtac cgccttgcgc ttaccgttgc ccaccagtat gttgaacagc
     1081 tcgaagaaaa tattaaaaat gccgtgttcg gcaatgtggg ctcgatgatt atctaccgcg
     1141 tcagccccga gaacgcagca ctctttgaaa agcagcttgc ccccgtgttt accccggccg
     1201 acattctgaa actcgagaac ttcaacgcgt atgcaaaaat tttagtgaac ggtgtaccgg
     1261 aaaagccctt caacctccgc attcccccac cgccaaaagg taacctcgaa gttaccgaga
     1321 aggtaaaaga gctctcgtac cttaaatacg gccgcccccg cgaggaagtg gaatccgaga
     1381 tcatggcacg gtataagtga tatactaagg aaatatggaa aacatggaaa aaccagtacc
     1441 tgattttgaa gattatcgaa atttatctga agatgctaca gcacagcaac gcataaacga
     1501 tattgtggaa cgagcaactg agcgaggtag cccactaacc aaagaggaaa gagatgaaat
     1561 taatcagtta aaaagccaca gagaagttga tgagttgatt gaaaaggcaa tacgaaatag
     1621 acctgatgga accgaagagt tctaaaaagc ttatgacata tagagaaatc gccacactat
     1681 aggtgtggcg attttgatta cagaaatatt atcctcttaa aaacatcttg cttttctaat
     1741 tgttctctta ccttttgccc ttggatatat atgtacattg cacacacacc aagtaaaaaa
     1801 ttaaagactc ctaataggat aaatacgaaa aggatttcat tttctgtcat aaagattgga
     1861 ttttgtgatt ttgaacattg atttcagata ccatagtaat atagtataaa tttttgtcaa
     1921 tttggacgga aaaaaccctt gcggtataat ggtcgtaggc tttaagcggg aacgcttagg
     1981 cactgacggt acacttacta gctaatacga gggatctccc tctttcgagt atgaataaac
     2041 aatcactcgt ggaatttctt cacagcaaat tcggcggcac caaggtgcag gctgaagaag
     2101 cagtggaggg cattattgca gaaatcacca aaaccttgaa gaaaggcgag gatgtttcga
     2161 tcgcaggcct gggcatcttt tcagtaaaga ctcgcgctgc tcgcatggca cgcaacccaa
     2221 agaccggcga acaggtaaaa gttgctgcaa cacgcgttgc ccgcttccgc cctgcaaagg
     2281 cactcaaaga ttcagttaaa taaactttct ctttcaaaaa taccccttcg gctacgctca
     2341 ggggtatttt tgttgcttga aataatggtg agtggtggta tagtgggaga aattattaaa
     2401 tatgcacaaa aacacaattc ttttcctaac taattttgaa aaaagagaat tttctggttt
     2461 cgaagctatt gcaaaaaagg tgctcttaca gcaacaaaac acttgtgtct cttttgttaa
     2521 tgaactcaaa accgcaccgg aaaccgatga gctcgtcaaa aagaaaaaac caaatttagt
     2581 catttttaaa gacattgcca ccgaattagt acaaagtact atcgcccgaa ttaaaaaaac
     2641 attgggtaac agtgttgaag ttgagttttt aacgcttggt aaaaaaatac ttccgaacgt
     2701 agaaatggtg tttgccgttg aagacatagg aaaaagatta gttgtgtgta attaaagtat
     2761 gtaggaccgg acataatgtt gttatgtccg gttttttgtg cgggataagg gatatctcac
     2821 ctcttggaaa cctgcggttt tccaacgctc gaagactcgc tgccttcctc cacggggaaa
     2881 cacccagttt ccccgacccc cttctggttc gactctctcc acactgcaaa caaaaaacac
     2941 cggaaagaat ccgatgtttt ttgtttgtgc gggataaggg agtcgaaccc ttcacctcag
     3001 tttggaaaac tgatgtttta ccgataaact aatcccgcat agggcataca aacagcccgt
     3061 aagaacgact ttatcctaac agatgagcga acccctttca aggagattat ttaagggtta
     3121 cttggtacaa ctttgtattt ggcactggcg cattgtcaga aaagaggagc atcgctgcgc
     3181 cgctcactgg tgttaccgga gcattaattt gtgccgtaaa gaaaagcggg taacgcggtg
     3241 ggttggtgat gctgtgcact cgaacgtatg aagggacgcc aagtgttgct cctgctgtat
     3301 tagcaagctg caccgtccca gcagcagtgt ttccgtctga aatcatccag gcacagcgct
     3361 gtttaataaa acctgaaaca ataaatggag cacttactac cttccccgcc tgcggcaccg
     3421 ttaccaccaa gcagtcattt gat
//
LOCUS       DATIBQ010000029         2679 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U16294
            k141_160868, whole genome shotgun sequence.
ACCESSION   DATIBQ010000029 DATIBQ010000000
VERSION     DATIBQ010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935789
            Sequence Read Archive: SRR25158331
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:58:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 637
            CDSs (total)                      :: 603
            Genes (coding)                    :: 601
            CDSs (with protein)               :: 601
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2679
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_160868"
                     /isolate="SMAG_U16294"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1839)
                     /locus_tag="VLB02_00875"
     CDS             complement(<1..1839)
                     /locus_tag="VLB02_00875"
                     /inference="COORDINATES: protein motif:HMM:NF036484.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56623.1"
                     /translation="MKKVEKEIQEITVRGARTHNLKNVSVTMPRNKMVAITGLSGSGK
                     SSLAFDTIFAEGQRRYVESLSSYARQFLNQMPKPDVDEITGLSPAISIDQKSRSNNPR
                     STVATITEIYDYLRVLFARVGKPFCPVCGTEIKKLSNEEIHNFVLAKIKERSKEKERE
                     VMGVTWNDIPVQIFAPVVRGRKGEYYQLLYDLLGKGYTAVRIDGTMHSLRDRVELSKN
                     SKHTIEVLVDSYAIHEFKDNPRDAELRLAEAIERALLEADGLVHIVFGKNQERITLPR
                     SSGRSTQPDTRQSDAFFLTENEFLLSSKFACPNDGYSFPEVEPRLFSFNSPYGACPTC
                     NGLGSKFFMGEEPCETCNGNRLRTEALHVFLGSGEKKKNVVEVTALSIEDAFDFFGGL
                     KLSPKDKEISRAVIKEVEDRLTFLLNVGLEYLTLNRTANTLSGGEAQRIRLASQLGSR
                     LVGAMYVLDEPTIGLHQRDNERLIKTLEDLRDLGNTIVVVEHDEDTIYASDYIVDIGP
                     GAGIHGGTVVASGWLEKLLAAKKNDTGSRTLAYLRKEEAITVPEARRTTEKGAIKIIG
                     ATIHNLQNQNIEIPLGRLTAITGVSGSGKSSFLYDVLYKNLQGRFER"
     gene            1897..>2679
                     /locus_tag="VLB02_00880"
     CDS             1897..>2679
                     /locus_tag="VLB02_00880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56624.1"
                     /translation="MKKVWLLFLFLLVPRLASAHVGYVIDQGTFNAHAGADATYFFSP
                     LIHGNTWILIATVIVAILAVYLLALRIRVTRQFFTSIHDRSVVYKLYIPWILRLSLGI
                     ALIGAATHHTLISPLATGSNALVTLELFVGFAFLLGAALLPATLITLILFCVALHTNA
                     YVMGNLEFLGAAVALLIAGSGKPGLDDMIGIRMPKLDTLKPLIPLFLRVSVGGALTFL
                     ALYEKVLNPHISEAVVIQYNLTNVIGVSPALWVFGVGIIELIV"
BASE COUNT          629 a          701 c          592 g          757 t
ORIGIN      
        1 gcgttcaaat cgcccttgca agtttttata gagcacgtca tagagaaagc tgcttttacc
       61 agagcccgaa acgccggtaa tagccgtaag tcgtcctagt gggatttcaa tattttgatt
      121 ctgtaagttg tgaatggttg caccaataat ttttatggca cccttttcag tagtgcggcg
      181 tgcttctgga acagtaattg cttcttcttt acgcaggtat gcaagggtgc gcgagccagt
      241 atcattcttt tttgcagcga gtagtttttc aagccaacct gacgcaacca ccgtgccacc
      301 atggattcca gcacccggac caatatctac tatatagtcg gaggcataaa ttgtatcctc
      361 atcgtgctcg accactacta tagtgttccc taagtcgcgc aaatcttcga gcgtttttat
      421 caggcgctcg ttgtcgcgtt ggtgcaaccc gatggttggc tcgtcgagta catacattgc
      481 acccaccagc cggctgccga gctggcttgc caaccgaatg cgctgtgctt ctccgcccga
      541 gagcgtgttt gcagtacgat taagagtaag gtactcaagc cctacattta atagaaaggt
      601 aagccgatct tcaacttctt ttatcaccgc tcgcgaaatt tctttatctt ttggcgaaag
      661 ttttaacccg ccaaaaaaat caaaggcatc ttcaatcgaa agcgctgtta cttcaaccac
      721 gttcttcttt ttttcaccac tacctaaaaa tacgtgcaat gcttcagtgc ggagccggtt
      781 accgttacac gtttcgcagg gctcttctcc cataaaaaat tttgagccta agccgttaca
      841 ggttgggcac gcaccgtagg gcgagttgaa tgagaagagc cgcggctcaa cctctggaaa
      901 cgagtagcca tcattcggac aggcaaattt acttgagagc aaaaattcat tttctgtaag
      961 aaaaaacgca tcactctgac gagtgtctgg ttgagtggaa cgacccgaag accgaggaag
     1021 cgtgatgcgt tcttgatttt ttccaaacac aatatgcacc agcccatcag cttctaagag
     1081 tgcgcgctcg attgcttctg caagccgcag ctccgcatca cgcggattgt ctttaaattc
     1141 gtgtatagcg tacgaatcaa ctagcacctc aatagtgtgc ttgctgtttt ttgagagttc
     1201 aactcgatca cggaggctgt gcatcgttcc atcaatgcgg accgctgtgt accctttgcc
     1261 gagcaggtca tagagaagct ggtagtactc gccctttctg ccgcgtacca ccggcgcaaa
     1321 aatctgtacc ggaatatcgt tccatgttac ccccatcact tcgcgctctt tttcttttga
     1381 acgttcttta atcttcgcca gaacaaagtt gtgtatttct tcgttagaga gtttttttat
     1441 ctcggtgccg cacaccggac aaaatggctt tcctactcgg gcaaagagca cgcgcagata
     1501 atcgtatatt tcagttatgg ttgccaccgt tgagcgggga ttatttgagc gcgatttttg
     1561 atcgatcgaa attgcaggag aaagccccgt gatttcatca acgtccggct tcggcatctg
     1621 gtttaaaaat tgccgcgcat aagatgagag tgattcaaca tagcggcgct gcccttcggc
     1681 aaaaatagtg tcgaacgcaa gcgagctttt gcccgagcca gagaggccag tgatcgccac
     1741 cattttattg cgcggcatcg ttactgaaac atttttcagg ttatgggtcc gggcgccgcg
     1801 taccgtaatt tcttgtatct ctttctccac tttcttcata tatatgatgc ggcgattcta
     1861 ccacaggaaa cccataagct gtatactgat agtgctatga aaaaagtatg gcttttgttt
     1921 ttgttccttt tagttccgcg tctcgcctcg gcacacgttg gctatgtgat tgaccagggt
     1981 accttcaacg cacatgcggg tgccgatgca acatactttt tttcacccct catccatggc
     2041 aacacatgga tactcatcgc caccgtcatt gtggctatac tcgctgtata cctgctcgcc
     2101 ttgcgcattc gagtgactcg ccagttcttt accagcatcc acgaccgctc agtcgtgtat
     2161 aagctctaca ttccctggat tttacggctc tcgctcggca ttgcactcat cggcgcagca
     2221 acacaccaca cgcttatctc gccactcgcc actggctcaa acgcactggt tacccttgag
     2281 ctctttgttg ggtttgcatt cttgctcggc gcggcactct tacccgcaac acttatcacc
     2341 ctcattttat tctgtgtcgc gcttcacacc aatgcatacg taatgggaaa tcttgagttc
     2401 ctcggtgcag cagtcgcact ccttattgca ggctccggca aaccaggact cgatgacatg
     2461 atcggcattc ggatgccaaa actcgatacc ttaaaaccac tcattccact ttttttgcga
     2521 gtcagcgttg gcggcgcact cacctttttg gcgctgtatg aaaaagtact gaacccacat
     2581 atttctgaag cagtagttat acaatacaat ctcacgaacg taattggagt gagccccgca
     2641 ctctgggtat tcggtgtcgg tatcattgaa ctcatcgtt
//
LOCUS       DATIBQ010000030         2771 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U16294
            k141_2233754, whole genome shotgun sequence.
ACCESSION   DATIBQ010000030 DATIBQ010000000
VERSION     DATIBQ010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935789
            Sequence Read Archive: SRR25158331
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:58:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 637
            CDSs (total)                      :: 603
            Genes (coding)                    :: 601
            CDSs (with protein)               :: 601
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2771
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2233754"
                     /isolate="SMAG_U16294"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(190..876)
                     /locus_tag="VLB02_00885"
     CDS             complement(190..876)
                     /locus_tag="VLB02_00885"
                     /inference="COORDINATES: protein motif:HMM:NF017373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56625.1"
                     /translation="MLIQTWSDVFATSLQGLWYGFISTVPAIIIAVIIFIIGWLVGVT
                     LSKAITQLVDALKIDRLFKGAGADDMLARAGWRLHVGKFLGEIVKWFVIIVFLTASLE
                     LVGLQQVNSFLRDVVLSYLPQVFIAALVLVIGVILAGAVKRLVSGGAMMANVRSANML
                     GSISYYAIWIFTILIALNQLGIAPQFMQILFTGIIAMMAVAGGIAFGLGGKEVAGRFL
                     GRLHDDMSQK"
     gene            complement(991..1623)
                     /locus_tag="VLB02_00890"
     CDS             complement(991..1623)
                     /locus_tag="VLB02_00890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56626.1"
                     /translation="MELLGKLLGGVPRVKIMRLFLLNPTQGFEVSDVADRSRLQPAAA
                     RHVIAQLAAMNFLKRRAFTKDVIDGRTERVKKKRVTGWFLNEEFPYLPELKTLLVEGE
                     FFKNDELVKRLRPAGKVQLLIVSGIFIQNSDSRLDLMIVGDNLRRSYIQKAIVVLESE
                     LGRELAYAVFDTNDFKYRISMYDKLIRDVLDYPHERLIVGKDFSTLLLPA"
     gene            complement(1643..2149)
                     /locus_tag="VLB02_00895"
     CDS             complement(1643..2149)
                     /locus_tag="VLB02_00895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56627.1"
                     /translation="MRYIFILFIVSSSFQGSAQTEDKLYDIFGYLTLGNRSFKEEKRG
                     LQKIFKDSAGEKFGCLWIPSPRNVLIAPVWCIGEKDMYERETVYVQQGRRWSKAVDIP
                     KGHRVEEIWWRGEFLIIKLKNCIDSTSEAVFEHYGGGRWKRVLPLKGKPMSFRHGFFL
                     VARSSNKL"
     gene            complement(2245..2676)
                     /locus_tag="VLB02_00900"
     CDS             complement(2245..2676)
                     /locus_tag="VLB02_00900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56628.1"
                     /translation="MKKVTVFLIFGSLLLACRTQKSSLLAEDGRLMLGGVPFTGTREQ
                     IAKIVSPDKPCPCDSFSKSKSIVVLERSLKRPTQTDSVGYAIFVQNKPGYYWKQIDFI
                     PYPAKISSLERVEYKKITLVLGQDTVQYVLTSRGRWKKTSK"
BASE COUNT          804 a          668 c          553 g          746 t
ORIGIN      
        1 gccgcaacac taaacaagtg gcttacggcc gtttattagt agttgtggtt ctcgtaaaca
       61 tatatatagg tgccaaggat tctatacgag gagaaagcga ctgtcaagca agtatggaat
      121 attcttttcc tgcaatacaa aacacccctc accggggtag ccggagaggg gtgtttttga
      181 gagaaaaacc tatttctgcg acatgtcgtc gtgcaagcga ccaaggaatc gcccagcgac
      241 ttctttgccg cccaaaccaa atgcaatacc tcctgctact gccatcatcg cgatgatgcc
      301 agtaaagaga atctgcataa actgcggcgc aatacccagc tgattcagtg caatcaagat
      361 cgtaaagatc cagattgcat agtatgaaat cgagccgagc atatttgccg agcgaacgtt
      421 cgccatcatc gcaccacctg aaaccaagcg ctttactgcg ccagcaagta tcacgccgat
      481 aacgagcaca agcgcagcaa taaacacctg cggaagataa cttaagacca cgtcacgcaa
      541 gaatgaattt acctgctgca agccaacaag ttcgagcgaa gcggtgagaa atacaataat
      601 tacaaaccac ttcacgattt cgcccaagaa cttgccaaca tgcaagcgcc agccagcgcg
      661 agcaagcata tcatcggcgc cagcaccctt gaagaggcgg tcaatcttga gcgcatcaac
      721 aagctgggta attgcttttg aaagcgttac cccaacaagc cagccgataa taaatatgat
      781 gactgcgata ataatcgcag gcacggtcga aataaatccg taccacaatc cttgtaagga
      841 tgtagcaaag acatcactcc acgtttgaat aagcataaaa acttctgtag cgaaaaactc
      901 gctactggtg cacacaacat tacgttaata aatctttact accttaaagt gtggccttta
      961 aagtacacgc ttattttacc acctctgggc ttaggctggt agcaggagtg tggaaaaatc
     1021 cttccccaca atcagtcgct cgtgcgggta gtccaaaacg tcgcggatga gcttatcgta
     1081 cattgaaatt cgatacttaa aatcattggt gtcaaatacg gcgtaggcaa gctcgcgacc
     1141 aagctcactc tcgagcacca caatcgcctt ttggatgtac gaacggcgta gattatcgcc
     1201 cacaatcatc aaatcgagcc gactgtcgct attttgaata aagatccctg aaacaatcag
     1261 cagctgcacc tttcctgcgg ggcggaggcg tttaacgagt tcatcgtttt taaagaactc
     1321 tccttcaacc aagagtgttt ttaattcagg caggtaggga aactcttcat ttaaaaacca
     1381 gccggtaaca cgcttctttt ttactctttc agttctgcca tcaataacat ctttggtaaa
     1441 agcacggcgc ttgagaaagt tcatcgcggc aagctgcgca ataacgtggc gcgctgcggc
     1501 aggttgcaga cgggagcggt cggcaacatc agagacttca aacccctggg tagggttcag
     1561 caaaaacagc cgcataattt ttacccgtgg cacgccgcct aatagttttc ctagtagttc
     1621 catactctct tcgatacgct tattatagtt tgttactact ccttgcaaca agaaaaaaac
     1681 cgtgtctgaa tgacatcggt tttcctttta agggaagtac tcttttccag cgcccgccgc
     1741 catagtgttc aaacaccgct tctgaggttg aatcgatgca gttttttaat ttgataatca
     1801 aaaattcacc acgccaccaa atctcttcga cacggtgacc ttttggtata tcaacagctt
     1861 tagaccaccg ccgcccctgc tgaacgtaaa cagtttcccg ttcatacata tctttctccc
     1921 ctatgcacca gactggagca attagcacat tgcgaggaga aggtatccag aggcatccaa
     1981 atttttctcc tgcactatct ttaaatattt tttgcagtcc ccgcttttct tccttaaaag
     2041 atcggtttcc cagtgtaagg tacccaaata tgtcatagag cttatcttcc gtctgagcac
     2101 tgccttgaaa gctgctgctc acaataaata gtatgaatat gtatctcata acaatgtgtt
     2161 tttctgtact ttacgccctt ttttgcgaga gtcaaataaa aaagcactcg catatcacga
     2221 gtgcctacca tcattgcttt tattttactt acttgttttt ttccatcgcc cccttgaggt
     2281 aagcacatat tgaacggtat cttgccctaa aacaagtgta atctttttgt actcaactcg
     2341 ttcaagagaa ctaattttag ccgggtaagg tatgaagtca atttgtttcc agtagtatcc
     2401 tggtttgttc tggacaaaaa ttgcatagcc tacactgtct gtttgagtag gtctcttaag
     2461 tgaccgctca agcaccacaa tactttttga ttttgaaaaa ctgtcgcagg ggcagggttt
     2521 atcaggtgat acaattttgg ctatttgctc tcgagttcca gtaaaaggga cgcccccgag
     2581 catcagcctt ccgtcttcag caagcagtga acttttctgg gttctacaag caagaagtaa
     2641 acttccaaaa ataagaaata cagtaacttt tttcataaaa cattttttct catattaaca
     2701 taattaattt aatttgtcaa taaatacaaa aacacccact acagtatgtg ggtgtttttg
     2761 ggagagactt t
//
LOCUS       DATIBQ010000031         3928 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U16294
            k141_1234134, whole genome shotgun sequence.
ACCESSION   DATIBQ010000031 DATIBQ010000000
VERSION     DATIBQ010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935789
            Sequence Read Archive: SRR25158331
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3928)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3928)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:58:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 637
            CDSs (total)                      :: 603
            Genes (coding)                    :: 601
            CDSs (with protein)               :: 601
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3928
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1234134"
                     /isolate="SMAG_U16294"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            107..301
                     /gene="rpmF"
                     /locus_tag="VLB02_00905"
     CDS             107..301
                     /gene="rpmF"
                     /locus_tag="VLB02_00905"
                     /inference="COORDINATES: protein motif:HMM:TIGR01031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HSE56629.1"
                     /translation="MRHTRAHTANRRSHHALKSLALTTDSKTGATHIRHRATKDGMYK
                     GKSVKDPVKKLQKKQKKAKK"
     gene            329..1237
                     /gene="nusB"
                     /locus_tag="VLB02_00910"
     CDS             329..1237
                     /gene="nusB"
                     /locus_tag="VLB02_00910"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HSE56630.1"
                     /translation="MANRHLSRSIVLQSLFSWDFMGRPADQLTEIVDRNIIEFAPGHN
                     DSEFIHYLADLVQKKQAVIDEIIQKAAPEWPLDKIANVDRSILRMGLAELLFGNRDQV
                     PPKVAINEAIELAKTYGGENSGRFVNGVLGAVYKEIGEPGKEQTSKQKVKAPEEPIDP
                     EKLPKERKAGGIVYAKHDGKYYLALVHDVFGYWTIAKGGVEGEETDEQTAVREIKEKL
                     GIEVTVKEKLGAIEYVASHPEKGKLLKIVTYFLAESEFKDLVLHSSGGLDNAAWLDPV
                     DLVDLPIYDDILPLIAKAIEYITNLK"
     gene            1308..2000
                     /gene="rnc"
                     /locus_tag="VLB02_00915"
     CDS             1308..2000
                     /gene="rnc"
                     /locus_tag="VLB02_00915"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HSE56631.1"
                     /translation="MIDFSLLEAALGITFKNKVFLQRAFTHRSYINENPKSGLTHNER
                     LEFLGDAVIELIVTDYLFTTYPEHTEGQLTAYRSALVNAVIMGEVANSLNMNEYLLLS
                     KGEKKDTGRARQTILANTYESFVGALYLDQGYGACNAFVTKTLIPKLADIISKELWRD
                     PKSRIQEAAQEYVGVTPAYKVVAETGPDHDKYFTIGIFFGSKKIAEGKGKSKQEAEQA
                     AAQAALDAQGWK"
     gene            2080..2427
                     /locus_tag="VLB02_00920"
     CDS             2080..2427
                     /locus_tag="VLB02_00920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56632.1"
                     /translation="MDRIVLKWEFDKKNDKEACLVLKEGAHSALFSNTVLILACNPPN
                     KKDNEKQASELQLKIKEQFSLLRTNVQLLVNKVLFDSAALAIVLQYPWKYYYRSIIER
                     KLKELIPEIEFIG"
     gene            2494..3030
                     /locus_tag="VLB02_00925"
     CDS             2494..3030
                     /locus_tag="VLB02_00925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56633.1"
                     /translation="MKKIIILLLVVAAVYAFFSRTPKTENEPVPQPVQQSLSPQAGLP
                     VVVEAKRQAIYRAARSGSYEQLAAEAETPFGYSYGDPETDFVTFLKNAATHEGKSALD
                     MIPLLLAQPYGVQGNIYVWPGVFAKSAEEWTDADIVQMKQLLTDEQIEGYRQFGAYAY
                     YRIGITTEGKWVYYIAGD"
     gene            3107..>3928
                     /locus_tag="VLB02_00930"
     CDS             3107..>3928
                     /locus_tag="VLB02_00930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56634.1"
                     /translation="MRRTVITILSAAALFFISPIFSSAYFTKFLVNMSAVQPTLVPFA
                     EHREPFTVSIDPSFRYYGGGEAPYRIERDSLCNQYDARYNCIDPVPNLCPYLSFESAS
                     GESTEHGFALNPSSNSRVADGELNDPSDTIDEWTLSVAAPCFEGECPAEYDRTRYGNP
                     LPIALKGKTFQCSIGVFSKDPPPLVQAASGIAFADTVSNEFTVSAIFAGATVVDTLTL
                     SLPTDAPFQSDGADPSIGTANSTPFEFKVLATDANNIPTTIKLILSDGATTTQHEM"
BASE COUNT         1141 a          874 c          913 g         1000 t
ORIGIN      
        1 atatatattt atatgtcaat agttcgcgta ttgaatataa aagtattaaa atttctattt
       61 gcatttttca aaaaatacgt tatgatgtcc acatatggta gttcgaatga gacacacccg
      121 ggcgcatacg gctaatcgcc gatcgcacca cgcactaaag agtttggcac taaccaccga
      181 ctcaaaaact ggtgccaccc acatccgcca ccgtgcaacg aaggacggta tgtataaagg
      241 taaatcagta aaagacccag tcaaaaaact gcaaaaaaag cagaagaaag ctaaaaaata
      301 atcggtaaca attagttctt ttaatcaaat ggcaaacaga cacctctcac gttccatagt
      361 cctccaatcc ctcttctcat gggattttat ggggcgccct gctgaccagc tgacagagat
      421 tgtcgatcgc aatatcattg aatttgctcc agggcacaac gatagcgagt ttattcacta
      481 cttggctgat ttggtgcaaa agaagcaggc ggtcattgat gaaatcatcc agaaggctgc
      541 acccgagtgg ccgctcgata aaatcgcaaa tgttgaccgc agtattttgc ggatggggct
      601 tgctgaacta ttgtttggta accgcgacca ggtgccgcca aaggtggcaa ttaacgaggc
      661 gattgagctc gctaaaacct acggcggaga aaacagcggt cggttcgtaa acggggtgct
      721 cggtgcagtg tacaaagaaa tcggcgagcc aggcaaagag cagacttcaa aacagaaggt
      781 gaaagcacct gaggagccga ttgacccaga gaagctccca aaagagcgca aggcgggcgg
      841 tattgtgtat gccaagcatg acggcaaata ctacttggcg ctggtgcacg atgtgttcgg
      901 ctactggacg attgccaagg gtggcgttga gggcgaagag accgacgagc aaactgccgt
      961 gcgtgaaata aaagaaaagc tcggcatcga ggttaccgtt aaagaaaagc tcggcgccat
     1021 tgagtatgtt gcctcgcacc ctgaaaaagg gaagctctta aaaattgtta cctacttctt
     1081 agcagaatcg gaatttaaag atttggtact acacagttcg ggggggctcg ataatgccgc
     1141 gtggctcgac ccggtcgacc tggttgactt accaatctac gatgatatat tgcctctgat
     1201 cgcaaaggcg attgaatata ttactaactt aaaatagtag tttgtagctt atagtttata
     1261 gggcacgtgt cctattacct gtaacctggt aactactcca aaaaacaatg attgattttt
     1321 ccctcctcga agcagcgctc ggcattacgt ttaaaaataa agtgttcttg cagcgcgcgt
     1381 tcacccaccg atcgtacatt aacgaaaatc caaaatcagg actcacccac aacgagcgcc
     1441 ttgagttttt gggcgatgcc gttatcgaac tcatcgtcac cgactatctc tttactactt
     1501 atcctgaaca caccgaaggg cagcttaccg catatcgctc ggcactggta aatgcggtta
     1561 tcatgggtga agttgcaaat agtttgaaca tgaatgaata cctgcttctt tcaaaaggag
     1621 aaaaaaaaga taccggtcgt gcgcggcaaa ctattttggc aaatacatac gaatcatttg
     1681 tgggtgcact gtatctcgac caagggtacg gcgcgtgcaa tgcgtttgtc accaaaaccc
     1741 tcattccaaa actcgccgat attatttcaa aagaactctg gcgcgatcca aagagcagaa
     1801 tccaagaagc agctcaggag tatgtgggtg taaccccagc gtacaaggtg gtggctgaga
     1861 ccggacccga ccacgacaag tacttcacca tcggcatctt ttttggcagc aaaaaaatcg
     1921 ccgagggaaa agggaagagc aagcaagaag ccgaacaggc agccgctcaa gccgcgctcg
     1981 atgctcaggg gtggaagtaa aaaatctcga ggagagcctc gggatttttt ttattgacta
     2041 atctaggtac catggtaggt tctgtaaaaa taaataacta tggacagaat tgttttgaaa
     2101 tgggaatttg ataaaaaaaa tgacaaagaa gcgtgtttgg tgttaaaaga aggagctcac
     2161 agcgcgctct tttcaaatac agtacttata cttgcatgta atcctccaaa caaaaaagat
     2221 aacgaaaaac aagcatcgga gttgcagtta aaaattaaag agcaattttc tctcctgaga
     2281 acgaacgtac agctgttggt aaataaagta ctgtttgatt cagctgcact tgctattgtt
     2341 ttacagtatc catggaagta ctactaccgg agtattattg aaagaaaatt aaaagaatta
     2401 attcctgaaa tagaatttat tggataagca tttgttccaa tttaataata aacccatagg
     2461 caagacctat gggttttgct gtatactgcc cctatgaaga aaataattat tctattgttg
     2521 gttgtagcgg cagtgtatgc gtttttctct cgcacgccta aaactgagaa cgagccagtc
     2581 ccgcagcctg tgcagcaaag cctttcacca caggcaggtt tgccggtagt ggtagaggcg
     2641 aagcggcagg caatataccg cgctgcccgt tccggcagct acgagcaact agccgcagaa
     2701 gctgaaacac cgtttgggta cagttacggt gatcccgaaa ctgattttgt tactttctta
     2761 aaaaatgctg ccacacatga aggtaaaagt gcgcttgata tgatcccgct attacttgca
     2821 caaccgtatg gggtgcaggg taatatttac gtgtggcccg gtgtgtttgc aaagagcgcc
     2881 gaagagtgga ccgatgccga tattgttcag atgaagcaac tcctcaccga cgagcaaatc
     2941 gaaggctacc gccagtttgg cgcctacgcc tattaccgta tcggcattac caccgaaggc
     3001 aagtgggtgt actatattgc aggggattaa aatattattt tactaggata tttttctctt
     3061 ttaagacttt atattttctt taactgttga tggtatattc tttaccatgc gacgtaccgt
     3121 aataacaatt cttagtgctg cggcactctt ttttattagc ccgatttttt caagtgcgta
     3181 ttttacaaag tttttggtga acatgtcggc agtgcagcca acgctagtgc catttgccga
     3241 gcatcgcgaa ccgtttacag tatcaattga tccatccttt cgttactatg gcggcggtga
     3301 agcgccctac cgtattgaac gcgattcact ctgcaaccag tacgatgctc ggtacaactg
     3361 cattgatccg gtgccgaact tgtgcccgta tctttcattt gaatcagcta gtggagagtc
     3421 gactgaacac gggtttgctc ttaatccgag ttctaactca cgcgttgctg acggcgagct
     3481 taatgatccg agtgatacca tagatgaatg gacactttca gtagcggcac cgtgttttga
     3541 gggcgaatgt ccggcagagt acgaccgcac tcgctacggc aacccgcttc ccatcgcgct
     3601 gaagggaaaa acatttcaat gttcaatcgg agttttttca aaagatccac ctccgctggt
     3661 acaggcagcg tctggtatag catttgctga cacagtttca aatgagttta cggtaagcgc
     3721 gatatttgcc ggagcaacag ttgttgatac gcttacactc tcgcttccca cagatgctcc
     3781 attccagagt gatggggcag atccaagtat cggcactgcc aactcaactc cgtttgagtt
     3841 taaagtgctt gcgactgatg cgaacaatat tcctacaact atcaaactga ttctttctga
     3901 tggtgccact actacacagc acgaaatg
//
LOCUS       DATIBQ010000032         5515 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U16294
            k141_1224516, whole genome shotgun sequence.
ACCESSION   DATIBQ010000032 DATIBQ010000000
VERSION     DATIBQ010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935789
            Sequence Read Archive: SRR25158331
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 5515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:58:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 637
            CDSs (total)                      :: 603
            Genes (coding)                    :: 601
            CDSs (with protein)               :: 601
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5515
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1224516"
                     /isolate="SMAG_U16294"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1450)
                     /locus_tag="VLB02_00935"
     CDS             complement(<1..1450)
                     /locus_tag="VLB02_00935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56635.1"
                     /translation="MNRKLLLLSVIFSLLAQSMTGLFSVVYATAGVPKIINFQGRLLN
                     SSGDLLGGPSGTNYCFRFSLYDNATVGAGSKLWPAGTPSTMTLSVREGVFNGNIGDTG
                     AGGDSLTYNFQDNDATYVHVEVAAQVASSCSGVSFEDLSPRQRIVSSGFAINSSTVGG
                     FTPAQSATGSQIPVLTSDTLILGGTSAGLKATSTNALTFQSGVTGDIQFFSSSNKITS
                     AGALTIAGNLSAAGLVINTDTITDLTGNGLAVSSGALVVNLTTSGTTGSASSNSGLEV
                     GSGGLTLLKGCADNEILKYTDAGGWACAADSSATVSDGDKGDVVVSSSGTVWSIDADV
                     IDWADIADATTLDADTSIVFGGSTFGLTFTNDGSDNETHNLTSTGDFVIQTGSNPFAT
                     FTDSGTVGIGTISPDRLFHTELSDAGTNAIAYPARFTHITSGTATTGFGLGQEFEAEN
                     GSGTNRIVGSFDFPFTDATDATEDTTFNLNLIR"
     gene            1534..2214
                     /locus_tag="VLB02_00940"
     CDS             1534..2214
                     /locus_tag="VLB02_00940"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HSE56636.1"
                     /translation="MNRFYLPTELDSGELTITDPRIVHQCKNVLRMEPGSMFSLFNGN
                     SIEWHATIREFGKNTCTVSVEGQLERETESERAVHAGIAILKKENFELVAQKLVEMGI
                     ASITPLISHRTIKNNIDRERVQKIMIEAAEQAGRLYVPELTEPASVADFLERQKTVAV
                     FQMNGIPVHEAKHASEITFMIGPEGGWSTEELELFSEKQLPHYSLGKTVLRAETAAII
                     GAHRLLWN"
     gene            2205..2972
                     /locus_tag="VLB02_00945"
     CDS             2205..2972
                     /locus_tag="VLB02_00945"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: protein motif:HMM:NF014703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HSE56637.1"
                     /translation="MELTIFHSIFLGLIEGLTEFIPVSSTAHLLITRALFGISHTAAT
                     DAFVIAIQSGAIIAALWYFWSAFKKDKSIFFKVCVAFIPTALIGLLLNNQIHTLFGSI
                     RTMSLALIIGGILLLLLPSKKEETSEEPISWNMAILLGLFQIAAFIPGMSRSGSLLIG
                     GVLLGLPRKQITAFSFLLALPTIFGATVVDVWNTKEFFTPSLILLTGIGALAACIVAL
                     LTMRFFLRFIEKPYALKTFGWYRIALGMLVLAVTFFK"
     gene            complement(2949..3545)
                     /locus_tag="VLB02_00950"
     CDS             complement(2949..3545)
                     /locus_tag="VLB02_00950"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF013514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HSE56638.1"
                     /translation="MKKWIIGVDEVGRGPIAGPVMVCACAFNPDTRRRLSFLGLTDSK
                     KMTLKAREAWFEKAKMWEEKGIIKYALAKRSSIVIDRCGISRAIKECVGEVLEKLCIT
                     PTDTQVLLDGSLYAPAEYTNQKTVIKGDAKHKEISLASVIAKVTRDRLMEQLHKKYPS
                     YLWFKNKGYGTKEHFLALQKDGKTTVHRSTFLLKKGNC"
     gene            3597..>5515
                     /gene="dnaE"
                     /locus_tag="VLB02_00955"
     CDS             3597..>5515
                     /gene="dnaE"
                     /locus_tag="VLB02_00955"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HSE56639.1"
                     /translation="MSFVHLHTHSHYSLLDGLSKIPELVKRAKEFGMPAVALTDHGSM
                     YGAIELYQECKKAGIKPIIGVEAYMAERSRFDKEPSIDGKRYHLTLLAKNNAGYKNLM
                     KLVSKANLEGYYYKPRMDEALLQEHSEGIICLSGCPGSRFITHVKNKNHEAAKALLEF
                     YIATFGRENVYVEVMNHKEVEWYVPLIPDIVALARELDLPIVGTWDSHYLHPEDAEAQ
                     DTLVAINTGSVVGESKISMKTGNYSFISPDEAKEMFKDIPGAFENTVKLAESVNIEIE
                     LSPWKFPTYPIPTDSNYDTELRKAVEQGLVEKAYEKTEEVATRIEFELDIIKQKGFSS
                     YFLVEADLVRAAREMGIYTNTRGSAAGSLVSYLTGITTVDPIKYKLPFERFLNPLRPG
                     IPDIDLDIADDRRDDLIGYVRKTYGEGAVAQICTFGTMAARGSVRDVARALGFPYAVG
                     DKISKLIPMGSQGFPMTIDHAIDIIPELKELYDTDRGTKEIIDMAKKIEGNVRHISVH
                     AAGVIISPTPDITEFTPIQFDPKGEGKIITQYDMFSGGRDGVVNLPKFDMLGIRNLQF
                     ISGTIDRVKKIRGIAIDIDTIPLDDKKVFEILARGETVGVFQMAGTGMTQYLKELKPE
                     KVEDLMAMVALYRPGP"
BASE COUNT         1511 a         1345 c         1234 g         1425 t
ORIGIN      
        1 ccctgatgag attaaggtta aacgtcgtat cttcagttgc atcagttgca tcggtaaatg
       61 ggaagtcgaa tgagccaacg attctgtttg tccctgatcc attctcagct tcaaattctt
      121 gaccaagacc gaagccggtt gttgctgttc cactggtaat atgagtgaat cgagcaggat
      181 aggcaattgc atttgttcct gcatcagaga gttcggtgtg gaagagacgg tcgggagaaa
      241 tagtgccgat accaacagta cctgagtcag taaacgtcgc aaatggatta ctaccggtct
      301 gaataacaaa atcaccagtc gaagttaaat tgtgtgtttc gttatcagaa ccatcgttgg
      361 taaacgtaag cccaaatgtt gagccgccaa acacaattga agtatcggca tcgagtgttg
      421 ttgcatctgc aatgtcagcc cagtcaatca catcagcatc aatactccat acagtaccag
      481 agctcgatac cactacatct cctttatcgc catcagaaac agtggcgcta ctatctgctg
      541 cacatgccca cccgccggca tcggtgtact ttaaaatttc gttatcggca caccctttca
      601 aaagggtaag cccgcctgaa ccaacttcta accctgagtt tgatgaggca gagccagtgg
      661 tgccactggt ggtgagattt accacgagtg cacctgagga aactgccaac ccgtttccgg
      721 ttaagtcagt aatcgtatca gtgttaatta ctaatcctgc tgccgaaaga ttacccgcaa
      781 tagtgagtgc gccagctgaa gtaattttgt tggagctcga gaaaaactga atgtcgcccg
      841 taacgccgct ttggaaggtg agcgcatttg tacttgttgc cttaagcccc gcagaggtac
      901 cgccaaggat taaggtatca gaggtaagca ccggaatctg actaccggtt gctgactgcg
      961 ctggcgtaaa gccgccaaca gttgaagagt tgattgcaaa accggaagag acaatgcgct
     1021 gccgcgggct caaatcttca aacgaaactc cggaacacga gctcgctacc tgcgccgcta
     1081 cttcaacatg cacataggtt gcatcgttat cttgaaagtt ataggtaaga gaatcaccgc
     1141 cggcaccagt atcaccaatg ttgccattaa aaaccccttc acgaactgag agcgtcatcg
     1201 ttgagggcgt tcctgctggc cacagttttg aaccagcgcc aaccgttgca ttgtcgtaga
     1261 gtgaaaatcg gaagcagtag ttggtaccac tcggaccgcc gagtaagtca ccgcttgagt
     1321 ttagaagtcg tccctgaaaa ttaattattt ttggaacacc ggcagttgca taaacaacag
     1381 aaaacaagcc cgtcatgctc tgtgcaagca gggaaaaaat aaccgaaaga agtagcagct
     1441 ttctgttcat cgctcagcct aaattgtacc atgtataaca ctcaaaaaat ccctcggata
     1501 ctgtgtatat tcgtgtatac tgccgctatg cgaatgaacc gattttacct ccctactgaa
     1561 cttgattctg gagaacttac tattactgac ccacgcattg ttcaccagtg taaaaacgtg
     1621 cttcgtatgg aaccaggcag tatgttttca ctcttcaacg gaaacagtat tgagtggcac
     1681 gcaaccatac gtgaatttgg taaaaatacg tgcacggttt ccgttgaagg acagttggaa
     1741 cgagaaaccg aatccgaacg tgccgtacac gcaggcattg caatcctcaa aaaagaaaac
     1801 tttgaacttg ttgcacaaaa attagtagaa atgggcattg cgagcatcac ccccctcatc
     1861 tcgcaccgaa ctataaaaaa caacatcgac cgcgagcggg tgcaaaaaat tatgattgaa
     1921 gcggctgaac aggcggggcg gctgtatgtg ccagagctga ctgaaccagc ctcggtagca
     1981 gactttcttg agcgtcagaa gactgttgca gtgtttcaga tgaacggcat tccggtacat
     2041 gaggcaaaac atgcctctga aataacattc atgattggtc ctgaaggcgg ctggagcact
     2101 gaagagcttg agctattttc cgagaagcaa ttacctcact actcgctcgg taaaacggta
     2161 ctgcgcgctg aaactgctgc aattattggc gcgcatcgac tactatggaa ctaacaattt
     2221 ttcattccat ttttttaggg ctcattgaag gattgactga atttattccg gtttcttcaa
     2281 ccgcacacct gctgattacc cgagcactct ttggtatttc acacacagcg gcgaccgatg
     2341 catttgtaat cgctattcag agcggcgcga ttattgctgc gctgtggtac ttttggagcg
     2401 cttttaaaaa agacaaaagc atttttttta aagtctgtgt cgcgtttatt cccactgctc
     2461 taattgggct tttattaaac aatcagattc acactctttt tggcagcatt cgcacgatga
     2521 gccttgcgct tattattggc ggaattctct tactgctctt gccaagtaaa aaagaagaaa
     2581 cttctgaaga acctatttca tggaacatgg caatcttgct cggactattt caaattgctg
     2641 cgtttattcc tggtatgtcc cgctcggggt cgcttctgat tggcggcgtt ttactaggac
     2701 tgccgcgtaa acaaattacc gcgtttagtt ttctcctcgc cctccctact atttttggcg
     2761 caaccgttgt tgacgtgtgg aatacgaaag aattttttac tccttcactc atacttctta
     2821 ctggcatcgg cgcactcgct gcttgcattg ttgcactgct caccatgcga tttttcttgc
     2881 ggtttataga aaaaccatac gcacttaaaa cattcggttg gtaccgcatt gcacttggaa
     2941 tgctcgtgct agcagttacc ttttttaagt aaaaaagtac ttcggtgcac cgttgttttt
     3001 ccatcttttt gcaacgctaa aaagtgctct tttgtcccat accccttgtt tttaaaccat
     3061 aggtagcttg gatatttttt atgcaattgc tccatcaaac ggtcgcgggt aacttttgca
     3121 attactgagg cgagtgaaat ttctttgtgt tttgcatccc ctttaataac cgttttttgg
     3181 ttcgtatatt cagcaggtgc atacagtgat ccgtctaaaa gtacttgagt atcagttggt
     3241 gtgatacaaa gtttttccaa tacttctcca acacactctt taatcgcccg agaaatcccg
     3301 cagcggtcaa taacgataga cgagcgtttc gcgagtgcat acttaataat tcccttctct
     3361 tcccacattt tcgccttttc aaaccatgct tcacgcgctt tcagtgtcat cttttttgag
     3421 tcagtgagcc ccaaaaacga gagtcgcctt cgagtatctg ggttaaacgc gcaggcgcac
     3481 accatcactg gtccggcaat cggtccccgc cctacttcat ccacgccaat aatccacttt
     3541 ttcatcccgt gtatcataac aaaaagcctt cctgttgggt ttggtatact tgccccatgt
     3601 cattcgtcca cctgcacact cactcgcact actcactttt ggacggactc tcaaaaatac
     3661 cagaactagt aaaacgagca aaagagttcg gcatgccggc agtggcgctc actgaccacg
     3721 gcagtatgta tggcgctatt gagctctacc aggagtgcaa aaaagcgggt atcaaaccaa
     3781 ttatcggcgt tgaagcatac atggcagaac ggagccggtt tgataaagag ccgagcatcg
     3841 acggcaaacg gtaccactta acgttacttg caaaaaacaa tgccggctat aaaaacctga
     3901 tgaagcttgt ttcaaaagca aacttagaag gctactacta caaaccccgc atggacgagg
     3961 cactgctgca ggagcacagt gaaggcatca tctgcctctc gggttgcccc ggaagccgat
     4021 ttattaccca tgtaaaaaac aaaaatcatg aggcagcaaa agcgctgctc gagttttaca
     4081 ttgccacctt cggcagagaa aatgtgtacg tcgaagtaat gaaccacaag gaagttgagt
     4141 ggtatgtgcc acttattccc gacattgttg cactcgcacg tgagcttgac ttaccgattg
     4201 tgggcacctg ggattcgcac tacctgcacc ctgaagatgc tgaagcgcaa gatacacttg
     4261 ttgctatcaa taccggaagt gttgtcggtg aaagcaaaat atcgatgaag actgggaact
     4321 actcattcat cagtcccgat gaagcaaaag aaatgtttaa agacatcccc ggagcatttg
     4381 aaaatacagt caaacttgct gaatcggtta atatcgaaat agagctctct ccgtggaaat
     4441 tccccaccta ccctattcct actgattcaa attacgacac ggaattgcgg aaagcagttg
     4501 aacaggggct cgtagaaaaa gcatacgaaa aaacagaaga agttgccaca cgcatagagt
     4561 ttgagctcga tattattaag caaaaagggt tctcttcata ctttttagtt gaagccgatt
     4621 tagtccgcgc agcacgagag atggggattt acaccaatac tcgaggctca gccgcgggct
     4681 cgcttgtttc gtacctcacc ggtattacta ctgttgaccc tatcaagtac aagctcccct
     4741 tcgagcgatt tttaaacccc ctccgccccg gtatccctga tatcgacctc gacattgctg
     4801 atgatcgacg agacgacttg attgggtatg ttcgtaaaac atacggagaa ggagcagtag
     4861 cgcagatatg tacctttgga acaatggcag cccgcggctc ggtgcgcgac gtggcacgcg
     4921 cccttggctt cccgtatgcg gttggtgata aaatttcaaa actcattccg atgggaagcc
     4981 aggggttccc catgaccatc gaccatgcta tcgatattat tcccgaacta aaagagctct
     5041 acgacactga ccgcggcacc aaagaaatta tcgacatggc aaaaaagatt gaagggaacg
     5101 tccggcacat ttcggtacat gccgccggag tgatcatctc tccgacccct gacatcaccg
     5161 agttcacccc cattcagttt gacccgaagg gtgaaggaaa aattattacc cagtacgaca
     5221 tgttttcggg cgggcgcgac ggcgtggtaa acctgccaaa gttcgacatg ctcggcatta
     5281 gaaacctgca gtttatttcc ggcaccatcg accgtgtcaa aaaaattcgg ggcattgcta
     5341 ttgatatcga taccattcca ctcgacgata aaaaagtgtt tgaaattctt gctcgaggcg
     5401 aaaccgtggg tgtattccaa atggcaggta ctgggatgac ccagtacctc aaagaactga
     5461 aaccagaaaa agtggaggat ttgatggcaa tggtcgcgct ctaccgcccc ggacc
//
LOCUS       DATIBQ010000033         2875 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U16294
            k141_2202501, whole genome shotgun sequence.
ACCESSION   DATIBQ010000033 DATIBQ010000000
VERSION     DATIBQ010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935789
            Sequence Read Archive: SRR25158331
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2875)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2875)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:58:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 637
            CDSs (total)                      :: 603
            Genes (coding)                    :: 601
            CDSs (with protein)               :: 601
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2875
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2202501"
                     /isolate="SMAG_U16294"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..428
                     /gene="lexA"
                     /locus_tag="VLB02_00960"
     CDS             <1..428
                     /gene="lexA"
                     /locus_tag="VLB02_00960"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR00498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HSE56640.1"
                     /translation="IIPSAIQSTSVSTINVPLVGSVSCGAPTLAEENIETYIPISTAL
                     AKKGSNYFLLRASGNSMNLAGINDKDILLVRQQDTAENGEKVVALINDEATVKFFEKT
                     DDAVILRPKSTDSMHMPIVLTDNCQVQGVVVAVLPSDLY"
     gene            433..642
                     /locus_tag="VLB02_00965"
     CDS             433..642
                     /locus_tag="VLB02_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006591176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56641.1"
                     /translation="MSKKYIRNAGKHWTPAEEKQLKILVKNNTPTRVIGLKLGRPVGG
                     VEGKASKLGVSLKPTNQSPYNRTKK"
     gene            646..1614
                     /locus_tag="VLB02_00970"
     CDS             646..1614
                     /locus_tag="VLB02_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56642.1"
                     /translation="MKKNIRNIPESILEKLKTINSKEIVAGCAVKFKSEFLLSEKLKH
                     LGIELTPDGLKVPSAVVPKAKQGKYSEKNIHGEEIIRKDLPKETHYNAIESPNWGDAS
                     NGTHTVYLPYQKYPREFQPPRELEISMICKDARPNLPVYVIAFQVKEVLDKGEKDFKK
                     KLLEDLNLLQENIGACGVEPADIKIAEYGKTLHLSWEILPPGTLDETLERIFKGKTPS
                     VEEKKVTAERYDFFQTLKPKSLVFGSSGFRRYFGALIQDNLVVFENIEYGNAIYILFN
                     NWEELSKRSRVEILSGKYGKDFKRVIHNAGWKQVVKKIVEEKRKLN"
     gene            complement(1765..1851)
                     /locus_tag="VLB02_00975"
     tRNA            complement(1765..1851)
                     /locus_tag="VLB02_00975"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1815..1817),aa:Ser,seq:cga)
     gene            complement(1992..>2875)
                     /locus_tag="VLB02_00980"
     CDS             complement(1992..>2875)
                     /locus_tag="VLB02_00980"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HSE56643.1"
                     /translation="YAGRKQLKTVVIAEEIGGQSAVSETIYNWIGTKAISGTDLAKNL
                     RSHIEEYTGEKGTLTLLIGKRANKVTKIDTGFSIELADGTMLSAKTVLVSTGSRHRKL
                     EVPGAKEFDNKGIMYCATCDGPLFTDMPVAVIGGGNAALEAAAQLTQYATSVTLLHRS
                     AEFRADPITIEQVLKDPKVTAIKNMELLSVEGDQFVEKLSYKIKGEETVHTIPVSGVF
                     VEIGQLPNTELVLGLADFDEFSRIKVDPYNQRTSVSGLWAAGDCTDVRYHQNNIAVGD
                     AIKAIEDIYLWIKRGSQ"
BASE COUNT          877 a          582 c          609 g          807 t
ORIGIN      
        1 gaataatacc ttctgcgatt caatcaacat cagtttcaac aatcaatgtt cctctcgtcg
       61 gttccgtatc ttgtggtgca cccactctag ccgaggaaaa tattgaaacg tacattccaa
      121 tttcaacagc tcttgccaaa aaaggatcaa attatttttt gctccgtgct agtggtaatt
      181 caatgaattt agctggcatc aatgacaaag atattctttt ggttcgtcag caagacacag
      241 cggaaaatgg agaaaaagtt gttgctctca taaatgatga agcgacagta aagttttttg
      301 aaaagactga cgatgcggta attctgagac caaaatctac tgattcaatg catatgccaa
      361 tcgtattaac agataattgt caggttcagg gtgttgtggt tgcagtactt ccttctgatc
      421 tgtattaaaa acatgtcaaa aaaatatatt cgtaacgctg gaaaacattg gacaccagca
      481 gaggaaaaac aacttaaaat attagtaaaa aataacacac caactcgtgt tattggtctt
      541 aagctcggtc gcccagttgg tggagtagaa ggcaaggcat cgaaactcgg tgttagtttg
      601 aaacccacga accaatctcc ttacaaccga accaaaaaat aaaatatgaa gaaaaatata
      661 agaaacattc cagaaagtat tttggaaaag ttaaagacga taaactcaaa agaaatcgtt
      721 gcaggatgtg ctgttaaatt taagagtgag ttcttattgt cagaaaaatt gaaacatctt
      781 gggatagaac ttacccctga tggtctcaag gttccttccg cagttgtgcc caaggcaaaa
      841 caaggaaagt attcagaaaa aaatattcac ggagaagaaa ttatacgaaa agatcttcca
      901 aaagaaactc attacaatgc gattgaatca cctaattggg gtgatgcatc taacggaact
      961 catactgtgt acttgcctta ccagaaatat ccccgagaat ttcagccacc ccgtgagtta
     1021 gaaatatcta tgatatgtaa agacgcacgt ccaaatctgc ctgtttacgt gatcgctttt
     1081 caagtcaaag aggttctaga taaaggagag aaagacttta aaaagaaatt acttgaggac
     1141 ttaaatcttc ttcaggagaa tatcggtgct tgtggagtag aaccagctga tataaaaata
     1201 gccgaatacg gcaagaccct ccatttatct tgggaaattc ttccgccggg aacgcttgat
     1261 gaaaccctag aacgtatatt taaagggaaa accccatcag ttgaagaaaa aaaagttaca
     1321 gcagaacgtt acgatttttt ccaaacttta aaaccaaaaa gtttagtatt tggtagtagc
     1381 ggattcagac gatatttcgg tgcccttatt caagacaact tagttgtctt tgagaatatt
     1441 gagtacggaa acgctattta tatcttgttt aataattggg aagaattaag taaacgaagt
     1501 agagttgaaa tactgtctgg gaaatatggt aaagacttta aacgtgtcat tcacaatgca
     1561 ggatggaagc aggtggtaaa aaaaatagtt gaagaaaaac gcaaattaaa ttagataaag
     1621 aatacaaaaa taagaagcac gtcatgcttc ttatttttgt attaggcact cgggtcttcc
     1681 gatactaaat actgtctgcg gagacggtga gatttgaact cacgatgagg tttcccccat
     1741 gccgcgtttt gttcttatcc aaagcggagg aggagggatt cgaaccctcg gtccagtttc
     1801 ccagactctc gatttcgagt cgagcacctt cgacctctca gtcactcctc cgctttggat
     1861 ataacaggac ttcaaccgct ccctcaagcc aatctcgtag gctcgataga ggcttgttct
     1921 ccgctccgct tcgaaacaga tgaaatatga agcagttcat atttcatagc cattcctccc
     1981 ttgtttaaat tttactgcga cccgcgcttg atccagaggt agatgtcttc aattgccttg
     2041 atggcatcac ctaccgcgat gttattttgg tgataccgta catcggtaca atcacccgca
     2101 gcccagagtc ctgaaacact ggtgcgttgg ttgtatggat cgactttgat tctactgaat
     2161 tcgtcaaagt cggcaagccc aagtacgagt tcggtatttg gtagctgccc aatctcaaca
     2221 aacactcctg aaacaggaat agtgtgtact gtttcttcac ctttgatttt atacgagagt
     2281 ttttcgacaa actggtcgcc ttcaactgaa aggagttcca tatttttgat ggcggttact
     2341 tttgggtctt tcagtacctg ttcgatagtg atggggtctg ctcgaaactc agcacttcgg
     2401 tgcaagaggg taacgctcgt tgcatactgg gtgagctggg ctgcggcttc aagcgcagca
     2461 ttgccgccgc caatgactgc gacgggcatg tcggtgaata gtggaccatc acaggtggcg
     2521 caatacatga tgcccttgtt gtcgaactct tttgcgccag gtacttcaag tttacggtgg
     2581 cggctgccag ttgaaacaag aaccgttttt gcagaaagca tcgtgccatc ggcaagttca
     2641 attgaaaacc ctgtatctat ttttgtaacc ttgttagcgc gctttcctat aagaagcgtg
     2701 agtgttcctt tttcaccagt gtactcttca atatgtgagc gcaggttttt tgcaagatca
     2761 gtgcctgaaa ttgccttagt accaatccag ttgtaaatgg tttcagaaac agcagattgt
     2821 ccgccaatct cttcggcaat gaccactgtt tttaattgct tgcgcccggc gtata
//
LOCUS       DATIBQ010000034         2438 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U16294
            k141_1864217, whole genome shotgun sequence.
ACCESSION   DATIBQ010000034 DATIBQ010000000
VERSION     DATIBQ010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935789
            Sequence Read Archive: SRR25158331
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2438)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:58:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 637
            CDSs (total)                      :: 603
            Genes (coding)                    :: 601
            CDSs (with protein)               :: 601
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2438
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1864217"
                     /isolate="SMAG_U16294"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(285..1013)
                     /locus_tag="VLB02_00985"
     CDS             complement(285..1013)
                     /locus_tag="VLB02_00985"
                     /inference="COORDINATES: protein motif:HMM:NF019826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HSE56644.1"
                     /translation="MSLINKDYKNKDFKKDVSFSLDTISKKGQKLLAALYLVTNHLSD
                     TDPLKLKIRTGAVLLPLVPHDSDSTNSIIRSEYETLLSAINTITALIQAAGMAQIVSD
                     KNTRILEAELHYFLKAIEERSGNTSNLSKTSELSTDFFNVAIEPVIKDIPKISKGHIE
                     KSFVLNRKDNSYNENLSQKSDRGAEIVSFINSKTIASIKDISALFPVVSEKTIQRELN
                     RLLAEGKIKKRGNKRWSMYLALGV"
     gene            1201..>2438
                     /locus_tag="VLB02_00990"
     CDS             1201..>2438
                     /locus_tag="VLB02_00990"
                     /inference="COORDINATES: protein motif:HMM:NF036827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HSE56645.1"
                     /translation="MAEDGRESRKKGKKKEAEAGTVAIRIIAGLLSLLLALVSIGGLL
                     GGAGRGGLFLFHGVWLLFGAGYIIFPVGLGTLGVFLLAGKRPETTVRQWVTLSVGLLA
                     SLAFLSIVSGSTLLGGYIGQGIAAPLVAVFDTLFAGLILFFALIGSFVIFFDQNPRIF
                     LEHVQQLVRAIRTLKLSKSAQPEENDQQEPVQYQEPEPTPAATSNTAPEPEPADDFVP
                     IQKKRKSGRYVPPPLSLLERDHGKANVGDVKANTNIIRRTLANFGIEVEMDEVTTGPT
                     VTRYALKPAQGVKLSRIAGLQNDLALALAAHPIRMELPIPGKSLVGIEIPNKTKATVG
                     LASLLSDPSFASNERPLVAAIGRNISGKPVLKSIAKMPHLLIAGTTGSGKSVTIHAII
                     ASLLYKHGPDDLRFIFVDPKR"
BASE COUNT          622 a          551 c          556 g          709 t
ORIGIN      
        1 tttcaatgat cgtcccgtgt ccagccgtac ttgtgcggca ccagcctgaa cgtgctgtaa
       61 ttactaaaat aatgcgccgt aaaagtacgg ctggacggat ttcataatag cacaaacagg
      121 ctttttttgc cagcagtgct attacgattt gcacattata ttattttgca agtcaacctg
      181 caaaatctct tgtggataaa aggtggatac atgaacgtgc acacacgcat ccgcagaagt
      241 aagacgaagc gcacgcgcaa gcattacgca acaaaacagt acacttacac accaagtgcc
      301 aagtacatgc tccagcgctt attgccccgt tttttaattt ttccttctgc caatagacgg
      361 tttagttctc gctgaattgt cttttcactc accactggaa agagggcaga aatgtctttt
      421 atggaggcaa tagtttttga gttaatgaac gaaactattt cagctcccct atcggacttt
      481 tgacttaaat tttcattata cgagttatct tttctattaa ggacaaaaga cttctcaata
      541 tgtccttttg atatctttgg tatgtctttt atgacaggtt ctattgcgac attgaaaaag
      601 tcagtgctta gttctgatgt ctttgatagg tttgaggtat tgccgctacg ctcctctatt
      661 gcttttaaaa agtagtggag ctctgcctcg aggatgcggg tatttttgtc agaaacaatc
      721 tgtgccattc cagccgcctg gataagtgcg gtaatagtat tgattgcgga gagtaaagtt
      781 tcatattcac tacgaataat cgaatttgta gaatcagaat cgtgcggtac cagcggaagc
      841 agcaccgcac cggtgcggat tttaagtttt agcggatctg tatcggacag gtggttggtt
      901 accaaataga gcgccgcgag gagtttttgt ccttttttag atatcgtgtc caatgaaaaa
      961 gacacgtcct ttttaaaatc tttgttttta tagtctttat ttattaaaga catatttttc
     1021 atttctcttt ccattaatta tttataattt aataggacac aatgtctttt tataagcaac
     1081 caaaaagatg tcttattata agagacatat tttttgggag caagttttta gtgtgttaga
     1141 aatgtcgtta ttgtataagg gttttttcca cacccgcgtg atatactgag ctgatacaca
     1201 atggctgaag atgggcgcga gtcgcgaaaa aaaggaaaga aaaaagaagc tgaagcagga
     1261 acagtagcaa ttcgcattat tgccggcttg ctctcgctct tgcttgccct ggtttcaata
     1321 ggggggcttt taggcggcgc tggcagaggc ggactgttct tgttccatgg cgtgtggctc
     1381 ctctttggtg cggggtacat tatctttcct gttgggttgg gcacactggg ggtattttta
     1441 cttgctggaa aacgccctga aactacggtg cggcagtggg taacgctctc cgttggactg
     1501 ctcgcgagcc tcgcctttct ctcgattgtt tctggctcta cactgctcgg cggctacatt
     1561 gggcaaggca tcgccgcgcc gttagtggca gtctttgata ctctcttcgc cggcctcatc
     1621 ctcttttttg cactaatcgg ctcgtttgtt attttctttg atcaaaatcc gcgaatattt
     1681 cttgagcatg tgcagcagct cgtgcgcgct attcgtacgc tcaagctttc aaaatcggca
     1741 cagccggaag agaacgatca gcaagaacca gtacagtatc aggaaccaga acctactcca
     1801 gcagcaacat caaacactgc tcctgaacca gagcctgctg acgactttgt accaattcaa
     1861 aagaaacgaa aatctggtcg ttatgttccg ccgccgctct cgctcttgga gcgcgaccac
     1921 ggcaaggcaa acgtaggcga cgtgaaggca aacacgaata ttatccgccg aacacttgca
     1981 aactttggca tcgaggttga aatggatgag gttactactg gtcccactgt tactcgttac
     2041 gcactcaaac cagcacaggg ggtaaaactc tcgcgcattg ccggactcca aaacgacctc
     2101 gcacttgcgc tcgccgcaca ccccatccgc atggagcttc cgattccggg gaagagtctg
     2161 gtgggtattg agattccaaa taaaaccaaa gcaacggttg ggcttgcgag cctgctctct
     2221 gacccgtcat ttgcgagcaa cgagcgcccc ctggttgctg ctattggtcg caatatttca
     2281 ggcaagccgg tactaaaaag tattgcaaaa atgccgcact tactgattgc aggtacaact
     2341 ggctcgggta aatcggttac gattcacgct attattgcaa gcctgctcta caaacatggt
     2401 cccgatgatt tgcgttttat ttttgttgat ccaaaacg
//
LOCUS       DATIBQ010000035         4313 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U16294
            k141_2013018, whole genome shotgun sequence.
ACCESSION   DATIBQ010000035 DATIBQ010000000
VERSION     DATIBQ010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935789
            Sequence Read Archive: SRR25158331
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 4313)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4313)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:58:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 637
            CDSs (total)                      :: 603
            Genes (coding)                    :: 601
            CDSs (with protein)               :: 601
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4313
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2013018"
                     /isolate="SMAG_U16294"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..497
                     /locus_tag="VLB02_00995"
     CDS             <1..497
                     /locus_tag="VLB02_00995"
                     /inference="COORDINATES: protein motif:HMM:NF023591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56646.1"
                     /translation="LIAFLGDGSFRDTLGILQKVLSGAGGRTIDRATVERITGAPPLT
                     IVSELFSALIEQNIDHCYTALRRAEEHNVDVATLIKLLLQAVRLTLMYRYSPSAREEI
                     AASYGEQYTELITRGAVEGTKSITSQSIISLITALERVSYAAIPTLPLELACIEITGT
                     EALT"
     gene            552..622
                     /locus_tag="VLB02_01000"
     tRNA            552..622
                     /locus_tag="VLB02_01000"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:584..586,aa:Gln,seq:ttg)
     gene            695..1900
                     /locus_tag="VLB02_01005"
     CDS             695..1900
                     /locus_tag="VLB02_01005"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HSE56647.1"
                     /translation="MKNLKIYFLIGVAVFGFLFFIPTGASAVVSCGISRTLEVGVEGE
                     DIRCLQKYLNESGFTITTTGVGSPGYESTQFKELTKKAVAKWQAANGIAPATGFFGPV
                     SRKKYLELVAGNTGKLPPASVPETNPVFAALNAKIASLTAELDAFRAGNAPTSSEDKK
                     VREALKKAIAMIIEAEDEIDSSDEHAAEAKGDLEDAREDFLEAVDAYLKNNFPKAVEL
                     ADDAFKNAEDAFEAAGGETEEDKIDEFIEEVEDKIQAAENKINGADDNDEDVEEAEQL
                     LEKASDTLDKAKDAFDNENFDEAENLAEKAEDLADDAVDAIGENSEKDDAKDALDDAR
                     DAIADAENEIEEADDDGKNTNEAENLLDEAKDKLDDAEEEFDDENYDEAQDLAKEAEN
                     LAQDAVDEL"
     gene            1956..2855
                     /locus_tag="VLB02_01010"
     CDS             1956..2855
                     /locus_tag="VLB02_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015324775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HSE56648.1"
                     /translation="MQKITPHLWFDTNAEEAAKFYTSIFKDGKIGAVARYPEAGKEVH
                     GMEPGTAMTVEFEIVGYKLLALNGGPIFTFTPAVSFTANCLSKEEVDELWEKLSEGGT
                     VLMPLDAYPFSERYGWLKDKYGLTWQLIFTPEAPSRTITPALMFTGGNCGKAEEAISF
                     YTQVFKDGKVGMIARYDAAHAPDKEGTIMYADFTLAGQRFSAMDSAREHTFTFNEAVS
                     LLITCENQEEIDYFWEKLSAVPESEQCGWLKDKYGVSWQIVPRGMGKMLTNPDKEKAN
                     RAMQAMLQMKKIDISKLQEAQEG"
     gene            complement(2898..3317)
                     /locus_tag="VLB02_01015"
     CDS             complement(2898..3317)
                     /locus_tag="VLB02_01015"
                     /inference="COORDINATES: protein motif:HMM:NF020157.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1761 domain-containing protein"
                     /protein_id="HSE56649.1"
                     /translation="MTVTLNGLAILGSMVLSVIIGFVWYGPLFGKRWMALSGIIMPPD
                     KKPCFKDMLKPILISLIGAFFMALCLSYSIAYAGAFMERFGTSMGIQAGFWSWLGFVV
                     PIQLSFVAWEGKPWKLFCIHAGYWLVLLCGMGIILSL"
     gene            complement(3435..3650)
                     /locus_tag="VLB02_01020"
     CDS             complement(3435..3650)
                     /locus_tag="VLB02_01020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56650.1"
                     /translation="MLSCTCGWEGVNLTPNHAQNTAHCPKCNTVFEGIPAEDAAPVSA
                     EQEEKITNSAGNFLKIVKALFPGFPSK"
     gene            4045..>4313
                     /locus_tag="VLB02_01025"
     CDS             4045..>4313
                     /locus_tag="VLB02_01025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56651.1"
                     /translation="MKTALIVHGMPSKEEYFDREGKSPSNSHWFPWIQQQLLINGFLA
                     QTPEMPEPYLPVYEKWCSVFEQFSIDEHATLIGHSCGAGFLLRWL"
BASE COUNT         1281 a          856 c         1032 g         1144 t
ORIGIN      
        1 cactcattgc cttcttaggt gatggatcat ttcgcgacac gctcgggata ctccaaaagg
       61 tgctctcggg ggcaggggga cggacaattg atcgtgcaac cgtcgagcgt atcactggtg
      121 cgccgccact cacgattgta agcgaacttt tctcggcact cattgagcag aatattgatc
      181 actgttacac cgcgctccgc cgggcagaag agcataatgt tgatgtagcg acgctcataa
      241 aattgctgct ccaagcagtg cggcttactc ttatgtaccg ctactcgccg agcgctcgcg
      301 aagaaattgc tgcctcatac ggcgagcagt ataccgagct catcactcgc ggcgccgttg
      361 agggtaccaa gagtattact tcgcagagca tcatctcgct catcaccgca cttgagcgcg
      421 taagctatgc cgccattcct acactgccgc tcgaacttgc ctgcattgaa atcaccggaa
      481 cagaggcttt aacataaggg ctttttatgt tataagaatg tggttcgtat attgtttttt
      541 gttttcaata ttggggaatc gtctaatggt aggacatcag gttttggtcc tgagtatcta
      601 ggttcgagtc ctagttcccc agcaaagaaa aaagaaacat aaatttattt ggtaacaatt
      661 gttaacattt ttaagcgagt atataatgct gtacatgaag aatctaaaaa tatatttttt
      721 aataggtgta gcggtgttcg ggttcttgtt ttttattcct accggagcat cggcggtagt
      781 ttcgtgtggc attagccgca cccttgaggt tggggtagaa ggcgaggata ttcgctgctt
      841 gcaaaagtat ttgaatgaat ctggttttac tatcactaca acaggggtcg gctctccagg
      901 ctatgaaagc acacaattta aagagctgac gaaaaaggca gtggctaaat ggcaggctgc
      961 caatggtatt gctccggcga cgggattttt tggacctgtt tctcgcaaaa aatacctcga
     1021 acttgttgct ggtaataccg gaaagcttcc tcctgcttca gtaccagaaa cgaatcctgt
     1081 gtttgctgca ttaaacgcta aaatagcatc tcttaccgca gagcttgatg cgttccgtgc
     1141 cggcaatgct cctacttctt cagaagataa aaaggtacgt gaagcgctca agaaggcgat
     1201 cgctatgata attgaggctg aagatgaaat agacagcagt gatgagcatg ctgctgaggc
     1261 taagggtgac cttgaagacg caagagaaga ttttcttgaa gcagttgatg cctaccttaa
     1321 gaataacttt ccaaaagcag tagaattagc ggatgatgcc ttcaagaatg ctgaagatgc
     1381 ctttgaagct gcaggaggcg agacagaaga ggataaaata gatgaattta tcgaagaagt
     1441 agaagacaag atccaggcgg cagagaataa gattaatgga gcggatgata acgatgaaga
     1501 tgtcgaagaa gcagagcagc tgcttgaaaa agcgagtgat acacttgata aggcgaagga
     1561 tgccttcgat aacgaaaatt ttgatgaggc tgaaaattta gcagaaaagg ctgaggatct
     1621 tgcagatgat gcggtagatg cgattggtga gaatagtgaa aaagatgatg caaaggatgc
     1681 tcttgatgat gcgcgggacg ccatagcgga tgctgagaac gagatcgaag aggcggatga
     1741 tgacggaaaa aacactaatg aggctgaaaa tttgcttgat gaagcaaaag acaagctcga
     1801 tgacgctgaa gaagaatttg atgacgaaaa ttatgatgaa gctcaagatc tggcgaaaga
     1861 ggcagaaaat ttggcacagg atgctgttga tgaactgtaa ttgcagttaa aagaaaaaat
     1921 cggcatgctt tgccgatttt tgctagaata tctatatgca aaaaataacc ccgcatttat
     1981 ggtttgatac aaacgctgaa gaagcagcaa agttttatac atctattttt aaagatggaa
     2041 aaatcggcgc agtagcgcgc taccctgaag caggcaaaga ggtgcacggt atggaaccgg
     2101 ggacagcgat gactgttgaa tttgaaatcg taggttataa acttcttgca ctaaacggtg
     2161 gtccaatttt cacgttcact cctgcagtat catttactgc aaactgtttg agcaaggaag
     2221 aggtagatga gctgtgggag aagttatcgg aaggcggcac tgtgctgatg ccgcttgatg
     2281 catacccatt cagcgagcga tacggctggc tcaaagataa gtatgggctt acctggcagt
     2341 taatttttac tcctgaagcg ccgagccgaa cgataactcc ggcacttatg tttactggtg
     2401 gtaactgcgg caaagcagaa gaggcgatca gtttttatac gcaggtattc aaagatggaa
     2461 aagttggcat gattgcccgc tatgatgcag ctcatgcacc cgataaagag gggacgatta
     2521 tgtatgccga ctttacgctt gcagggcagc gattctcggc aatggacagt gctcgagaac
     2581 atacatttac gtttaatgaa gcagtatcac ttttaataac ctgtgaaaat caggaagaaa
     2641 ttgactactt ctgggaaaaa ctttctgctg ttcctgaatc tgaacagtgt ggctggctca
     2701 aagacaaata tggtgtttcg tggcagattg tgccgagggg gatgggaaag atgcttacta
     2761 atcccgacaa agaaaaggca aaccgcgcta tgcaggcaat gctccagatg aaaaagattg
     2821 atatatcgaa attgcaagag gcgcaggaag gataaacata aaaaaatccc gataatggga
     2881 gttttttatg gtgcaaatta gagtgaaaga ataattccca tcccgcacag gagcacgagc
     2941 cagtaaccag cgtggatgca gaagagcttc catggtttac cttcccaggc aacgaagctt
     3001 agttgaatgg gaactacaaa cccgagccag ctccaaaacc cagcttggat gcccatactc
     3061 gtgccgaagc gctccatgaa cgcgccagcg taggcaatcg agtagctgag acatagtgcc
     3121 ataaagaatg caccgatgag cgaaataagt atcggcttca gcatatcttt aaagcatggt
     3181 tttttgtcgg gcggcataat aatccccgaa agcgccatcc accgcttgcc aaacagtggt
     3241 ccgtaccaca caaagccaat aataaccgag agcaccattg aaccaagaat agctaaaccg
     3301 ttaagtgtta ctgtcatatg ttatgtactt aatttgtaat agctaaagta tataaggtga
     3361 aggctaaaat aacgagatta gataaacaaa accccgtaca cgtacggggt ttttgataca
     3421 gttcattttt gtttttattt gctcggaaac cccggaaata gtgctttaac gatttttaaa
     3481 aaatttccag cactattggt aatcttttct tcttgttctg ctgaaacagg tgcagcatct
     3541 tctgcgggga taccttcaaa gactgtatta catttggggc aatgagcagt gttttgcgca
     3601 tggtttggtg ttaaattcac cccttcccat ccgcatgtac aggacaacat gacaacctcc
     3661 ttatttagtt atttaaaaag tttctttaaa caaaaatatt acggttcaat atatcattat
     3721 tttataaaga gtcaagtgcc ccgcaccttc ttatctactc cggaattcta ccaatcagat
     3781 attttctttc tcgtaaaaca cccaaccgcc agattcatgc tggcggttgg gtgtttagct
     3841 atattcagta ttttgttttc tttgcagata gggtgtcggt accatagtac ccttccatac
     3901 gtgcttcgtg tcgttattct tttaaatgta caaacaaata accctgtgta ctttttacca
     3961 cactgagata aaatcctgct gtagtattta taaaaattat tactcatagc tttttcagat
     4021 aacatcttct agaatactaa ctctatgaaa acagcactta ttgtccatgg gatgccttct
     4081 aaagaggagt attttgatcg ggaaggtaaa tctccttcaa atagtcactg gtttccatgg
     4141 atacagcagc agctattgat taacggattc cttgcgcaga caccagaaat gccggagccg
     4201 tacttgccgg tatatgaaaa atggtgctcg gtttttgaac aatttagcat agatgagcat
     4261 gccactttga tagggcacag ctgtggcgcc ggttttttgt tgcgctggtt aag
//
LOCUS       DATIBQ010000036         2577 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U16294
            k141_861681, whole genome shotgun sequence.
ACCESSION   DATIBQ010000036 DATIBQ010000000
VERSION     DATIBQ010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935789
            Sequence Read Archive: SRR25158331
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:58:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 637
            CDSs (total)                      :: 603
            Genes (coding)                    :: 601
            CDSs (with protein)               :: 601
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2577
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_861681"
                     /isolate="SMAG_U16294"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..690
                     /locus_tag="VLB02_01030"
     CDS             <1..690
                     /locus_tag="VLB02_01030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56652.1"
                     /translation="NPLVPLHIFLTNKQICIILNKNSTMKYLKRKLKSIVLYFRIHNI
                     GKAKNKLSKPVSFMLKNLEEQQEKTYPGTIERIVKSLHEAVSLLHSFNITLEAMERKI
                     DLKRVLVHFPATKILFDFIKEYKKGEHSINRCPPHLKHLSIDHVYITAQLRVGAGEES
                     APISILAEKISLGKASQGLVYQAVLNEINKINTFLYQPSDQGTLLQVIKKELSLQNQT
                     SYRKTKTQKTV"
     gene            complement(724..990)
                     /locus_tag="VLB02_01035"
     CDS             complement(724..990)
                     /locus_tag="VLB02_01035"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HSE56653.1"
                     /translation="MKKKQLFKERVLAIVAKIPKGKTLTYGQVAALAGSPNAARAVGT
                     IMKSNYLPHVPCHRVIRADGKIGDYNRGGAAAKEALLRKEKALR"
     gene            complement(968..1570)
                     /locus_tag="VLB02_01040"
     CDS             complement(968..1570)
                     /locus_tag="VLB02_01040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56654.1"
                     /translation="MKKKVFLILILAVVIVLVVVLVRRDKKVEAPKDALYLNWNSYET
                     EQYSLQYPKDFSFSEEYHYQALGPGKGITGIAFTIPVILTKNTNLSKDTKLSIETIPN
                     KEDCTPADFLSAPTVKEPLTENGIQILVAEQSEGAAGNRYDETVYVYPKCVAVRYFIH
                     STNIGNYTPGTVQEFDRAALLQAFTLIRASFQIHEKEAAI"
     gene            complement(1574..1957)
                     /locus_tag="VLB02_01045"
     CDS             complement(1574..1957)
                     /locus_tag="VLB02_01045"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HSE56655.1"
                     /translation="MEYLFLLSRILFGGYFLWNGYKHFKHTANLAGYAASKKVPSPQL
                     MVRLSGALLLIGGVGIVLGAAVGLAVSALVAFLIPVTFMMHDYWKETDPVQRAHQQIA
                     FAKNIALLGAAFAYLFIPGPWFLAI"
     gene            complement(1978..2370)
                     /locus_tag="VLB02_01050"
     CDS             complement(1978..2370)
                     /locus_tag="VLB02_01050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56656.1"
                     /translation="MSKIFGIVLTIIVVALLGGAVWMHQNRVQDTIGDDSAWNDYMNK
                     QVTIEGTLTCLPHKDTSGPTTLECAFGLVTDAGQYYAFDTSRTDLEQTGDRVRVTGVV
                     TPVESLSANQWQNYNIVGIITVASVQKI"
BASE COUNT          767 a          606 c          476 g          728 t
ORIGIN      
        1 aatccattag taccactgca catttttttg acaaataaac aaatctgtat tattctgaat
       61 aaaaatagta ctatgaaata tctcaagaga aaattaaaaa gcattgtgct ttactttcgc
      121 atacacaaca ttggtaaagc aaaaaacaaa ctttcaaaac cagtttcttt tatgctcaaa
      181 aatctggaag agcagcaaga aaaaacctac ccgggaacaa tagaacggat agtaaaatct
      241 ctccacgagg cagtatctct gcttcatagt tttaatatca cccttgaagc tatggagaga
      301 aaaattgacc taaaaagggt actcgttcat tttcctgcaa caaaaatact ctttgatttc
      361 atcaaggagt ataaaaaagg agagcactct atcaatcggt gcccgcctca cctaaaacat
      421 ctatcgatag accatgtcta tattacagca cagttacgag tgggtgcagg ggaagaatca
      481 gctccaattt caattttggc agaaaaaatt tcacttggta aagcgagtca aggattagtg
      541 taccaagcag ttctcaatga gataaataag attaatactt ttctgtatca accttctgat
      601 caagggacac tcttacaagt aatcaaaaaa gaattgtctc ttcaaaatca aacatcttat
      661 agaaaaacca aaacacaaaa aactgtttag taaaaaaatt agtaaagtcc ctgcactagc
      721 acgctagcgc agggcttttt cttttcgcag gagcgcttct tttgcagcag cgccgccgcg
      781 attatagtcg ccaatctttc catcggcgcg gatcactcgg tgacagggta catgtggcag
      841 gtagttgctt ttcataatcg tccccactgc tcgtgctgca ttcgggctcc ccgccagtgc
      901 tgccacttgc ccgtaggtaa gcgtttttcc ttttgggatt ttcgctacaa ttgccagcac
      961 ccgctcttta aatagctgct tctttttcat gtatctggaa tgatgcccga ataagggtaa
     1021 atgcttgcag cagcgctgcg cgatcaaatt cctgcactgt ccccggggtg taatttccaa
     1081 tattcgtgct gtgaataaaa taccgaaccg cgacacactt tggatacaca tacaccgttt
     1141 catcatagcg gttccctgca gccccttcgc tttgctctgc cactaaaatt tgtatcccgt
     1201 tttctgtaag tggttccttt acggtaggag cagaaagaaa gtcagcaggt gtacagtctt
     1261 ctttgttcgg aattgtttca atactcaatt ttgtatcttt agagaggtta gtatttttag
     1321 taagtattac tggaatcgta aacgcaatac cggtaattcc cttgcccgga ccaagtgctt
     1381 gatagtgata ctcttcgctg aatgaaaaat ctttagggta ctggagcgag tactgctctg
     1441 tttcgtaact attccaattc agatatagtg catccttcgg cgcttctact tttttatcgc
     1501 gccgcaccaa tacaacaaca agcacaatca ctactgcgag tatgagaatg agaaatactt
     1561 tctttttcat agcttaaata gcgagaaacc acggtcctgg aataaacagg tacgcaaacg
     1621 ctgcacccaa aagcgcaata ttttttgcaa acgcaatctg ctgatgcgct cgctgcactg
     1681 gatcagtttc tttccagtag tcgtgcatca taaatgttac cggaatgaga aatgccacca
     1741 gtgccgacac cgcaagccca accgcagcgc cgagcacaat acccacaccg ccaataagta
     1801 acagtgcccc gctcaagcgc accatgagct gcggagacgg aacttttttt gatgcagcgt
     1861 accctgcaag atttgcagta tgcttaaagt gcttgtaccc attccacaaa aagtatcctc
     1921 caaataaaat tctgctgagt aaaaataagt attccatggt tattcctcgt ttagtagtta
     1981 tattttttgc acactcgcta ccgtgatgat gcccacaatg ttgtaattct gccactggtt
     2041 agcgctcagc gattctactg gtgttaccac ccctgttacc cgaacacgat cacccgtctg
     2101 ttccaagtct gttcggctcg tatcaaacgc atagtactgc cctgcatcag tcacgagccc
     2161 aaacgcacac tcaagcgttg ttggaccact agtatcttta tgcggcaaac aagttagtgt
     2221 tccctcaata gttacctgtt tattcatgta gtcgttccag gcactatcat ccccaatcgt
     2281 atcctgtaca cgattctgat gcatccacac tgcacctcct aataacgcta ctacaataat
     2341 ggtgagcaca attccaaata ttttactcat accattaacc ataccctctc caaaaaaatg
     2401 atgcaagagg gtagaagact attctagaac aactgttaaa agctcaggga atttttcggt
     2461 ttgtaaatct tcaaaacaaa aatgaccttt gtcggtaaat tctctaaacg ttgcatgtgg
     2521 tagcttttct tttaatagtg caactgtttg caaaactaca gcatcgtcat caataga
//
LOCUS       DATIBQ010000037         5302 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U16294
            k141_347303, whole genome shotgun sequence.
ACCESSION   DATIBQ010000037 DATIBQ010000000
VERSION     DATIBQ010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935789
            Sequence Read Archive: SRR25158331
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 5302)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5302)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:58:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 637
            CDSs (total)                      :: 603
            Genes (coding)                    :: 601
            CDSs (with protein)               :: 601
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5302
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_347303"
                     /isolate="SMAG_U16294"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..201)
                     /locus_tag="VLB02_01055"
     CDS             complement(<1..201)
                     /locus_tag="VLB02_01055"
                     /inference="COORDINATES: protein motif:HMM:NF039185.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="double zinc ribbon domain-containing protein"
                     /protein_id="HSE56657.1"
                     /translation="MQLLSSIGRTVLSVLFPPRCAGCKKPGSTLCSQCAARLEPARGI
                     TAPWYSASYSYGDKLVRTVLTDL"
     gene            220..1344
                     /gene="pilM"
                     /locus_tag="VLB02_01060"
     CDS             220..1344
                     /gene="pilM"
                     /locus_tag="VLB02_01060"
                     /inference="COORDINATES: protein motif:HMM:TIGR01175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HSE56658.1"
                     /translation="MNFLDRMKSLFSLGSSSNVAVGIDIGTSSIKVVQVKREGGRAVL
                     DTYGALTLGPYVEGGFLGAVTNLPPELLAKALKDVLKETNITTTNVVLGIPSLSCIIF
                     IIQLPAVIDEASLATVVPNEAKKFIPVPLSDVSLDWYTIPRREDSSSSATGALTAAGG
                     EQKISVLVVATLNETLVRYTQTLQKSGLPFDSLEIDVFSQIRSVIARELSPVLLMDIG
                     ASKTKLAVIEHGIVETFHLVNRGGQDMSLSVARSLELPFDRAELLKKETGILPSTDHP
                     QIQEILTTHFTDIFSETNSILLAYEKRYNKSVSKIILSGGGALTRGLLEYAKGQFSAE
                     VVLADPFAKMEAPVFLQGVLKTTGPEFSIAIGLALKKLQQ"
     gene            1423..2082
                     /locus_tag="VLB02_01065"
     CDS             1423..2082
                     /locus_tag="VLB02_01065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56659.1"
                     /translation="MEPNIPTSFIPKRPLAEEPAHRMSQNPLGLVSLITTVLFFATIL
                     ASGGIFLYERQLMAQRAQMEVEINKAKEGLGAEFITDMKKLDNRITAIKELIQNHIVV
                     SPIFEALQDSTLRSVQYKNFAYAFGSAAGSSQRVVSVEMNGTAKNYETIALQSEAFSK
                     SSVIKDAVFSNLTVDEKKGVVNFSLRFSVAAADLSYQAFIDALQRPAPGALTNPNATT
                     L"
     gene            2079..2645
                     /locus_tag="VLB02_01070"
     CDS             2079..2645
                     /locus_tag="VLB02_01070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56660.1"
                     /translation="MIRFLLFIIIIGGSIAAFLLYINPHYKNVRQLRQEALSYTAALE
                     NAKKLREARDELQARYNAIPGDQIEAVETILPDNVDNIRLIIQLNSIALKTGMSSLRE
                     VDYSTDTPASASSARATNDRLAGDFQIKFSTTGSYKNFLNFLSTLEANLRLVDVNKIS
                     FDVVEDPTKGTFDYNKYTVTLKTYWLKN"
     gene            2647..3348
                     /locus_tag="VLB02_01075"
     CDS             2647..3348
                     /locus_tag="VLB02_01075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56661.1"
                     /translation="MATSRTKKIIIGVAIVAVAAVGATMIGGKDTPAPTTGGLVSNRV
                     DSGIAVGTQPTAGNDEFLATLLSIRSIRVDTSLFKNPAFLYLKDHPVVLGTETVGRSN
                     PFAPIGNDPVFEAPAGTISVGGQQQPAAPSSSSQTAGVETLQPGKITAKSAEFGAVVS
                     FPNGTGPVVLVFDYGTTEAVPYSTESVILESGDRILLPLTGLTAGTRYYVRASLLYGS
                     QTLRGSVMSFTTKTQ"
     gene            3355..5088
                     /locus_tag="VLB02_01080"
     CDS             3355..5088
                     /locus_tag="VLB02_01080"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HSE56662.1"
                     /translation="MTFLDYLLKQTVITQPQYQKVLDTIDTEFHGNVTTAVASLGIAP
                     DVLTQAMAGFYRIPYKKVSATEVPSEVLRYIEEDAARHYKFVPIAFSDGVLSVGVTEP
                     DNIETMNALQFISAKLGVPFKIFLIAEQDLLQILENYKGVRSEVDAAITELNTELVEA
                     NEEVAKEKKTKQKEEDKANVEKIVEDAPIIKIVAVIINHAIEGDASDIHIEHTGDKVK
                     VRYRVDGTLHTTLVLPLNTFDAIIARVKILSKLRLDEKRKPQDGSFATMIEGRKIEFR
                     VSTFPAYYGEKVVMRILDSERGVKPLESLDFSEWNYQLVTEAIAKPHGLILITGPTGS
                     GKSTTLYSMLNTVDREGNNVVSLEDPVEYQVAGISQSQVMPEIGYTFANGLRSILRQD
                     PDIIMVGEIRDKETAQLAIQAALTGHLVFSTLHTNTAIGAIPRLVDMGIDPYLIAPTL
                     RLIIAQRLVRKVHPSARKKVPMDHATQELVTKQFADLPEEFRKKFSTGDSMYEIVPSP
                     ECPSGTRGRLPVFEMFAVDKDMQELILKNPVEPELYKLLRAKGMLTLREDALLKALKG
                     DIMMQEVFSFG"
BASE COUNT         1394 a         1267 c         1316 g         1325 t
ORIGIN      
        1 aaggtcagta agcacagtac gaacgagctt gtcaccatat gaataactgg cgctgtacca
       61 gggtgcggta attccgcggg ctggctcaag ccgtgccgcg cactggctgc acagcgtgct
      121 acccggtttt ttgcagccag cacagcgagg cgggaagagt actgaaagta cggttcgtcc
      181 tatagaagat agtagctgca tagcgtatac tatagtgtaa tgaattttct ggatcgaatg
      241 aagagtctgt tttccctcgg cagcagcagt aatgttgcag tagggatcga catcggcacc
      301 tcttctataa aagtggtaca ggttaagcgt gaagggggga gagcagtgct cgatacctat
      361 ggcgcgctca cgcttggacc atatgttgaa ggaggctttc ttggtgcggt aacaaatttg
      421 ccgcccgagc tcctcgccaa ggcgctcaaa gatgtattaa aagaaacaaa catcactacc
      481 acgaacgtgg tacttggcat tccctcactc tcgtgcatta tttttattat ccagctgccg
      541 gcggtgattg atgaagcaag cttggcaacg gtagtaccga atgaggcaaa aaagtttatt
      601 ccggtgccgc tctcggacgt gtcactcgac tggtatacga ttccgcggcg agaagattct
      661 tcaagttctg caacgggggc actcacggct gccggcggtg agcaaaaaat ttcagtgctt
      721 gtggtagcaa cactgaacga aacacttgtg cgctacaccc aaacactaca aaaaagcggg
      781 ctgccgttcg attcgctcga gattgatgtg ttctcgcaga ttcgctctgt cattgcgcgt
      841 gaactctcgc cggtactgtt aatggatatt ggtgcatcaa aaacaaaact cgcggttatc
      901 gaacacggta ttgttgagac cttccacctg gtgaatcgcg gcgggcaaga catgagtctc
      961 tcggttgcgc ggagcttgga gcttccattt gaccgcgccg aactgctgaa aaaagaaacc
     1021 ggaattttgc caagtactga ccatccccag attcaagaaa tccttacaac gcacttcacc
     1081 gacatatttt ccgaaacaaa cagtattttg ctcgcctatg agaagcgcta caacaagagt
     1141 gtttcaaaaa ttattctttc gggcggcggc gcgctcactc gcgggctttt agagtatgca
     1201 aaggggcagt tctcagccga ggtagtgctc gctgatccgt ttgcaaaaat ggaggcgccg
     1261 gtatttttgc agggcgtact aaaaacaact ggaccagaat tctcgattgc tattggtctt
     1321 gcgcttaaaa agttacagca gtaggttttt taccacgcta tacacagtat gtagtgcatt
     1381 ttttttcaac cgtgcgtgat atacttttca tgtactctac ttatggaacc aaacatccct
     1441 acatcgttca ttccaaaacg cccccttgct gaagaaccgg cacatcgcat gtcgcagaat
     1501 ccgctcggtc ttgtttcgct catcaccacc gtgctctttt ttgcgactat tctcgcttcg
     1561 ggcggcatct ttttgtacga gcggcagctt atggcacagc gtgcgcaaat ggaagtagag
     1621 attaataaag cgaaagaggg attgggagca gaattcatta ccgatatgaa aaagctcgac
     1681 aatcggatta ctgccattaa agagcttatt caaaaccata ttgtggtgag tcctattttc
     1741 gaggcgctgc aagattccac cctgcgctcg gtacagtaca aaaattttgc atacgctttt
     1801 gggtcagcag ccggatcaag tcagcgtgtg gtgagtgttg aaatgaacgg taccgcaaaa
     1861 aattacgaaa ccatcgcact tcaatccgag gcattcagca agagttctgt tattaaagac
     1921 gcggtgttct caaacctcac cgttgatgaa aagaaagggg tggtgaactt tagcttgcgt
     1981 tttagcgttg ctgcagctga cctctcgtac caggcgttta tcgatgcgct tcagcgtcct
     2041 gcacccggag cacttaccaa ccctaacgca accaccctat gatccgattt ctccttttta
     2101 ttatcattat tggcggatcc attgcggcgt tcctcctcta cattaatccg cactataaaa
     2161 atgtgcggca gcttcggcaa gaggcgctct catatactgc agcacttgag aatgcaaaaa
     2221 agctgcgaga agcccgcgat gaacttcagg cacgctacaa cgctattccg ggtgatcaaa
     2281 ttgaagcagt tgaaacaata ctgcccgata atgtcgacaa cattcggctg atcattcaac
     2341 taaattccat tgcgctcaaa acgggcatgt catccctgcg tgaggtcgat tattcgaccg
     2401 atacccctgc tagtgcttcc tcagcgcgtg ctaccaatga tcgtctcgcg ggggactttc
     2461 agattaagtt ttcaaccacc ggctcgtaca aaaattttct taactttctc tcgacactcg
     2521 aggcaaacct gcgcttggtt gatgtgaata aaatctcatt tgatgtcgtt gaagacccaa
     2581 ccaaaggtac tttcgattat aataagtaca ccgttaccct taaaacgtat tggctcaaaa
     2641 actaacatgg caaccagtcg aacaaaaaaa ataattatcg gggtagcgat cgtcgcggtt
     2701 gctgctgttg gggcgacaat gatcggaggc aaagataccc ctgcgccaac caccggaggg
     2761 ctggtatcaa acagggttga ttcaggtatt gcagttggta ctcagcctac tgctggcaat
     2821 gatgaatttt tggcaacact gttgagtatt cgcagtatcc gtgttgatac ctcgctcttc
     2881 aaaaaccctg catttttgta cttgaaagat catcccgtcg tgctcggtac tgaaacggtt
     2941 ggccgctcaa acccgtttgc gccgattggc aacgatccag tgtttgaggc acctgccgga
     3001 actatttctg tcggcggaca gcaacaacct gcagcgcctt caagctcaag ccagactgct
     3061 ggtgttgaga cactccagcc aggaaaaatt accgcaaaaa gcgccgagtt tggtgcggtc
     3121 gttagttttc ctaatggtac tggtccggta gtgcttgtgt ttgattacgg aacaactgaa
     3181 gctgtgccgt actcaactga atcagttatt cttgaatcag gtgatcgtat tctcttgccg
     3241 ctgacggggc ttactgcggg tacacggtac tatgttcgcg cgagcctcct ctacggatca
     3301 caaacgctcc gcgggtcggt tatgagcttt actactaaaa cgcaataatc cagaatgacg
     3361 tttcttgatt atctcctcaa gcaaaccgta attacgcagc cgcagtatca aaaagtgctc
     3421 gacacgattg atactgaatt tcacggaaat gttaccaccg cggtagcatc gctcggcatt
     3481 gctcctgacg tgcttactca ggcaatggcg gggttttacc gtattccgta caaaaaagtg
     3541 tctgctaccg aagtaccttc agaggtgctt cgctacattg aagaagatgc agcgcggcac
     3601 tacaagtttg tgcctattgc gttctcagac ggcgtgctct cggtaggggt gactgagcct
     3661 gacaatatcg aaacaatgaa tgcgctccag tttatctctg cgaaacttgg ggtaccgttt
     3721 aaaatatttt tgattgccga gcaagatctg ctccagatac tggaaaatta caaaggagtg
     3781 cggagtgaag ttgatgccgc cattaccgag ttgaatactg aacttgttga agcgaatgaa
     3841 gaagtcgcca aagaaaagaa gacaaagcag aaagaggaag ataaagccaa tgttgagaaa
     3901 atagttgaag atgcgccgat tattaagatt gtggcagtga tcatcaatca cgcgatcgaa
     3961 ggggatgcgt ctgacattca catcgagcac acgggtgata aggtgaaggt tcgctaccgt
     4021 gtcgacggca cgctccacac caccctggtg ctgccgctca acaccttcga tgcaatcatt
     4081 gcgcgggtaa aaattctctc gaagctgcgg ctcgatgaaa agcgcaagcc gcaagacggt
     4141 agttttgcca ccatgattga ggggcgcaaa atcgaatttc gtgtttcaac cttccctgcg
     4201 tactatggcg aaaaggtggt gatgcgtatt ttggattctg agcggggagt caagccgctc
     4261 gaatcgctcg atttttcaga gtggaattac cagctggtaa ctgaagcaat tgccaaaccg
     4321 cacgggctca ttctcatcac tggtccaaca gggtccggaa aatcaaccac gctctactcg
     4381 atgctaaaca cggtggaccg tgagggaaac aatgtggttt cgctcgaaga tccggttgag
     4441 tatcaggttg ccggtatttc gcaatcacaa gtgatgcccg aaattgggta cacctttgct
     4501 aatgggctcc gctcaatttt gcgccaagac cccgacatta tcatggtggg agaaatccgc
     4561 gataaagaga cagcgcagct cgctatccaa gcagcgctca ccgggcacct ggtgttctcg
     4621 acacttcaca ccaacactgc tattggtgcc attccgcgat tggttgatat gggaattgat
     4681 ccgtacctca ttgccccaac gcttcgactg attattgcgc agcggttggt gcgcaaggtg
     4741 cacccgagcg cgagaaaaaa agtgccgatg gaccacgcta cccaagaact tgttactaaa
     4801 cagtttgctg atctccctga ggagttccga aagaagtttt ccactggaga ctctatgtat
     4861 gaaatagtac catcgcccga gtgtccgtcg ggtacccgcg gcaggttgcc ggtatttgaa
     4921 atgtttgcgg tcgataaaga catgcaagag ctgatcttga aaaatccggt tgagcctgaa
     4981 ctgtacaaac tgttgcgggc aaagggaatg ctcacactcc gcgaagatgc actcctgaag
     5041 gcactaaaag gggacatcat gatgcaggaa gtgttctcgt ttgggtaaaa gcgtttgtgg
     5101 tacaagagca agagcagtat acttaaacag gtagcatgga acaggaaaag caaaaaacaa
     5161 tattcattgt tgacgatgat aattttttgc tcgatatgta tgcgctccgg tttacccagg
     5221 caggatttat ggtgaagacc gcatcaaatg ctgatcaagt gctcgaagca ctccatggag
     5281 ggttggtgcc agacgtcctc tt
//
LOCUS       DATIBQ010000038        11594 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U16294
            k141_1186294, whole genome shotgun sequence.
ACCESSION   DATIBQ010000038 DATIBQ010000000
VERSION     DATIBQ010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935789
            Sequence Read Archive: SRR25158331
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 11594)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11594)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:58:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 637
            CDSs (total)                      :: 603
            Genes (coding)                    :: 601
            CDSs (with protein)               :: 601
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11594
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1186294"
                     /isolate="SMAG_U16294"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2971)
                     /locus_tag="VLB02_01085"
     CDS             complement(<1..2971)
                     /locus_tag="VLB02_01085"
                     /inference="COORDINATES: protein motif:HMM:NF012271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HSE56663.1"
                     /translation="MKKIVSVVLAVLTAFSFVLYKSLPVDAAAPSAVTTLAAGSGDTS
                     AFLSWTAPANGGSAITDYVIGYRTIGAGSWTTFSDGTSTVTTTTVTGLTNGTRYEFNV
                     QAVNGDGTATDSNNPTTIPLKKIITDDFESYVTSTDVSGQNGWLPGDTAASVLWKVYS
                     RSGSKVVAPDTGTSSADFTKFAIHQSAGQNAHIGFDFSGFTESGRFFPQVWFGRSTGS
                     GNDQGYDTFLWPGSGGDQLIVWKHAAGDGGAGPTVADESFTMTNGTIYRVEAEIITNA
                     SNQPVITIWIYPKSGSRGAPALTYTDTSGSPFTGSYLGLGFATLESFPPATDVWYDNI
                     DIYGTEAGPAAAPVISSTSTASGQVTVNWSAPFYTGTSAITDYNIQYKLTSDSSWTTF
                     SDGTSTTTNAAVTGLTNALSYDFRVRAVNSAGAGSYSPLAQATPAVGATAPTASSVAF
                     TGYPAVGKTLTGSYTYSDANGDSEGTSTFRWLRASSSGGSYSAIGGATSSTYVVQAAD
                     ENQFLKFEVTPVATVAPATGSAALSSASNKVFSEAAYYHILSTGQSLAVGYNSTTALT
                     TTQPYNNLMLTNGVEATSTPLIALRESGNGENGNVETPSSAMANTIRALDVFGRPFIV
                     GLHGHGGAPYNDIKKGTSYYSRGMTQATNSASYVTGTLGGVYYPRAVTLTHGETDYDN
                     GVSAATYQGYLEEMQNDYETDLNTIAGTSGTIPLFINQMNAGLTGTIAVSQLDAHRDN
                     TDSIILVGPKYQMEYASDHVHLTNTGSKYLGELMGKVMEKVLLQGESWNPLMPTAVSR
                     VGAVITASFAIPTGTLAIDTTLVAQRPNYGFEFTQTGGNSPTISSVSLTNNDTEVQVT
                     LSATPTGTNQRLRYAYTCTGNHTGAYDFLCGDADDGTYVGGNIRDTDSTTSSATGSTG
                     LVLYDWLVAFDEPITSDTTAPTVSSITSTTASGTYTPGDMINITVTFSEAVTTTGTVT
                     VNLNSGGSCTITASSSTT"
     gene            complement(3071..4321)
                     /locus_tag="VLB02_01090"
     CDS             complement(3071..4321)
                     /locus_tag="VLB02_01090"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HSE56664.1"
                     /translation="MYKNEIRGGSKTASYAPSRARTQFGPRRPFNGSRPPFRGGGGAR
                     NKRKTVFIDETLYINKAKECVLEVYTPTHTFADFGLHETIVSNLVARKYVTPSPIQDQ
                     AIPVVMQGKDVIGIANTGTGKTAAFLLPLVHKLAENPHQKVMVLAPTRELAQQIEKEL
                     LMFKRGFKLWSVTCVGGAPIGSQMRELERGVHFVIGTPGRVKDLINRGKIKMPEFKTI
                     VLDEADRMMDMGFIADMRYILKLMAPERQSLFFSATMSPSVKTLCGEFLREPVTISVK
                     TRDTAQNVDQDVVRVESKEHKIEALHDLLIKPECAKVLIFREMKRHVDDLGRTLRDRG
                     FKALALHGDMRNRQRELAVRSLAKGEVQVLVATDVAARGIDIPDITHVINYDVPQDYE
                     TYIHRIGRTGRGSSKGTALTFVHK"
     gene            complement(4544..5302)
                     /locus_tag="VLB02_01095"
     CDS             complement(4544..5302)
                     /locus_tag="VLB02_01095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56665.1"
                     /translation="MTPNQFGTEEKLFTEFRSGNRIAGNYLFSQLFNPLVSYAMQNGC
                     VPATARKVTVKALNTFRFEYVLCKAESINEAQNHLKEKIMLLLEDIPSTEVAKKEQAQ
                     GSKKKRRKKLVVEKFDYQRFYDQLKDMGEKIYEKRISLGISQEMCAKQVDINIKVLID
                     IEQGKGVRFQDFRWIFKFFGYCPLEKPDTPSEKILHYRYVHGLNQESFGNLFNVSTPA
                     VSGWEKYGNVPGAVLSFLADNPIPYFIDGMLVNV"
     gene            complement(5388..6023)
                     /locus_tag="VLB02_01100"
     CDS             complement(5388..6023)
                     /locus_tag="VLB02_01100"
                     /inference="COORDINATES: protein motif:HMM:NF014724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ElyC/SanA/YdcF family protein"
                     /protein_id="HSE56666.1"
                     /translation="MNKKLLRILALAAFLMLVLAWFADRQIESTAQDFVYTQMQDIPY
                     NKAGLLLGTAKTLSSGQQNLYFEYRIQAAVELFQAGKIEYLVISGDNSRKEYNEPEDM
                     KIALVARGIPESKLYLDYAGFRTYDSVVRMEKIFGQTNFTIISQAFHNERAIYIARSR
                     NLEVIGFNAQDAYLNADIKTKIREKFARVKAVLDVFFRKEPKFLGEKIEIK"
     gene            complement(6094..6167)
                     /locus_tag="VLB02_01105"
     tRNA            complement(6094..6167)
                     /locus_tag="VLB02_01105"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6131..6133),aa:Asp,seq:gtc)
     gene            complement(6185..6258)
                     /locus_tag="VLB02_01110"
     tRNA            complement(6185..6258)
                     /locus_tag="VLB02_01110"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6222..6224),aa:Met,seq:cat)
     gene            complement(6249..6941)
                     /locus_tag="VLB02_01115"
     CDS             complement(6249..6941)
                     /locus_tag="VLB02_01115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56667.1"
                     /translation="MALYQKRMQAHTLRKKGMPIGVIAQTLSVSKGTASMWCRGITLS
                     DTQKKAILKNSLKKTSKGRMLGALMNKQKRLDAIAAADSYGKKTIKKLAPKELLLIAT
                     ALYWAEGSKSDSTAGFQFINSDPVMILCIKKFLLTNGISHQELFCTIKINQVHKPRIN
                     DVLKFWKKLLGLTSEQMGNPSFVKTEGKKVYANHDTYFGICRLKVRKSTALKYKVLGL
                     IKALKNDILLPV"
     gene            complement(6968..7210)
                     /locus_tag="VLB02_01120"
     CDS             complement(6968..7210)
                     /locus_tag="VLB02_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005540634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HSE56668.1"
                     /translation="MWYMYILVCADESYYVGVTNNIERRFLQHVQGTGAKYTRSHKPV
                     SCVYSEPFHTRSTAQKREAEIKRWPRAKKEQLVARS"
     gene            complement(7238..7308)
                     /locus_tag="VLB02_01125"
     tRNA            complement(7238..7308)
                     /locus_tag="VLB02_01125"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7274..7276),aa:Met,seq:cat)
     gene            complement(7344..7943)
                     /locus_tag="VLB02_01130"
     CDS             complement(7344..7943)
                     /locus_tag="VLB02_01130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56669.1"
                     /translation="MYGPDIPEPVFLNLEYVFFLLYRTFHGVIDYILTGAFWNDLKVL
                     ATIIVILLITAILYCLIRLHEIKKEQQKKTAADAAEKLLAASTAVPVAKNEAWEAMRR
                     RMLSDDESEWRLGIIEADILLDRTLTKKGYQGDTLSDKLKQIGPEKIASVQLAWEAHK
                     IRNRIAHEGADFTFTLPEVRRALANFEAVFKELGVIDVA"
     gene            complement(7959..9347)
                     /gene="gatA"
                     /locus_tag="VLB02_01135"
     CDS             complement(7959..9347)
                     /gene="gatA"
                     /locus_tag="VLB02_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010872228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HSE56670.1"
                     /translation="MVTILELHKKIVSGEVKITELVGEYVARAKADTLNCFREIFSDI
                     DEQVQKAEAMLANGTATLLTGIPVAIKDNIMFAGHIAGACSHMLEHYTAAYDSTVVRE
                     LKKAGAVIIGRTNMDEGAMGSSTENSAYGVTKNPHDETRVPGGSSGGSAAAVAANLVV
                     AAFGSDTGGSIRQPAAFCGVAGLLPTYGTVSRHGLIAMASSLDVIGPFAGTVEDVEFL
                     YKAVAVPEPLDSTSVARTETTPVLKKRLAIPKGIFENGGCDKEVQENFETTIEKLKKE
                     GFVIDEIELPHFVDALAVYYIIQPAEVSSNLSRLDGVRYGDRMSDASVSEMYKQTRGQ
                     LFGKEVRRRILLGTYVLSHGYYDAYYNKAVALRALIRTELEGACTTYDAIITPTTPTP
                     AFKIGEKAHDPVAMYLSDLFTVPANIAQVPALSVPNGKTNAGLPLGFQCIGPRFGEHT
                     LFELGKIIESIR"
     gene            complement(9349..9612)
                     /gene="gatC"
                     /locus_tag="VLB02_01140"
     CDS             complement(9349..9612)
                     /gene="gatC"
                     /locus_tag="VLB02_01140"
                     /inference="COORDINATES: protein motif:HMM:NF014716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HSE56671.1"
                     /translation="MIDVQKLADLARLEVPKDEQEAVAKDLETIIGFVDQVQSRDVSA
                     AAGYNSVNVFREDEVAPLESVYDLVAAAPLHQDGFVKVPKVIE"
     gene            9712..9975
                     /locus_tag="VLB02_01145"
     CDS             9712..9975
                     /locus_tag="VLB02_01145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56672.1"
                     /translation="MTKTLVELQESETFTNADLIETMKHSVENKLEINPSLVMHLINR
                     VELGDIERNTMWELAKKGRDEQIDKRNKKAADGYEMIMGYLHE"
     gene            complement(10022..>11594)
                     /gene="ligA"
                     /locus_tag="VLB02_01150"
     CDS             complement(10022..>11594)
                     /gene="ligA"
                     /locus_tag="VLB02_01150"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HSE56673.1"
                     /translation="TENIKVISDIPLRLLPPYPQHIEVRGEVVMQKSVLERLNKEQEE
                     KGLPKFANTRNAAAGSVRQLDPSVAQSRQLSFFAYDIATLHGAQKIPEFHSEEHALLR
                     ELGFPVTSQECVAKTLPEIETFIAAISGARDQLPYHIDGIVVQVDELSLHDRLGVVGK
                     APRYAVAFKYPAERATTTVTAITTQVGRTGVLTPLAHFKPTLVAGSTVSKATLHNMDQ
                     IERLGIRVGDTVVIQKAGDVIPEVVQVLTELRTGKEKKFSMPKKCPECEGEVAKRESE
                     TGKGVAYYCTNPKCPAKNRRGLIHFVNVFEIYTVGPKIIDRLQDEGLITDAADLFTLE
                     ESDLSGLERFGEKSAENIISSIQGKKQVQLARFIYALGILHVGEETARDLANECKTFE
                     KFWNAPAEALDAIPNIGPAVIKSIAEYRRRPEAKRFIEKLFKQGVVVLPVKDDKKPKP
                     FVGKTFVLTGTLETLSREEAKQKILERGGNVSSSVSKATSYVVAGENPGSKLTEAQKH
                     GIPVLDEGKFLKMVK"
BASE COUNT         3031 a         2809 c         2479 g         3275 t
ORIGIN      
        1 ctgttgtact actgcttgca gtaatggtac atgagccgcc agaatttaaa ttcaccgtta
       61 ctgtccctgt agtggtaact gcctctgaga aggtgacggt aatgtttatc atgtcccccg
      121 gtgtgtaggt accacttgct gttgttgaag ttatagaaga aactgttggc gcagttgtat
      181 cagaagtaat tggttcatca aatgccacga gccagtcgta gagcaccaaa ccagtgcttc
      241 cggttgcgct tgaggtagta ctgtcggtat cacggatatt tccaccaaca taggtaccgt
      301 catcagcatc accgcagagg aaatcgtacg caccggtgtg attaccagta caagtatatg
      361 cgtagcgaag gcgttgattg gtaccggtag gagtagctga aagggttacc tgtacctcgg
      421 tgtcgttatt cgttaaactc acgcttgaga tagtgggaga atttccgcct gtttgggtaa
      481 attcaaatcc ataattaggg cgctgtgcaa caagtgtggt atcgatcgcg agtgttccgg
      541 ttggaatagc aaatgaagca gtgataacag caccaactcg gcttaccgct gttggcatta
      601 gtggattcca cgattctccc tgtaaaagaa ctttctccat cactttcccc atcaactcgc
      661 ctaaatattt tgagccggta ttagtgaggt gtacgtgatc gctcgcatac tccatctggt
      721 acttcggtcc caccaaaata atactgtcag tgttatcgcg gtgtgcatca agttgcgaaa
      781 cggcaatagt accggtaagc cctgcgttca tctggtttat aaaaagtgga attgtcccgc
      841 tcgttccggc aatggtgttt aaatcagttt cgtaatcatt ttgcatttct tccaaatagc
      901 cttggtacgt tgctgctgaa acaccattat cataatcggt ttcgccatgg gtgagtgtta
      961 ccgcacgcgg atagtacact ccaccgagcg tgccggtaac ataacttgct gagttggttg
     1021 cctgggtcat accacggctg tagtagctcg ttcccttttt aatgtcgttg taaggagcac
     1081 cgccgtgacc atgaagccct acaataaatg gccgaccaaa tacatcgagt gcacgaatcg
     1141 tgtttgccat agcagacgat ggcgtttcaa cattgccatt ttcaccgttc cccgattcgc
     1201 gaagcgcgat gagcggcgtg ctggttgcct caacaccatt agtgagcatt aagttattgt
     1261 atggctgagt agtagtgagt gcggtcgtag aattgtaccc aacagccaaa ctctgccccg
     1321 ttgaaagaat atggtagtag gctgcttcag aaaatacttt gtttgatgca ctgctgagcg
     1381 ctgcagagcc ggtagcagga gcaacggtcg caactggtgt aacctcaaac ttaagaaatt
     1441 gattctcatc tgctgcctgc acgacatagg ttgaactggt tgcaccgccg attgctgaat
     1501 agctgccgcc cgaggaagat gcgcgcaacc agcggaatgt tgacgttccc tcgctatcac
     1561 cattcgcgtc gctatacgta tatgaaccgg tgagtgtttt gccaaccgct gggtaccccg
     1621 taaatgcaac cgatgaagca gttggcgcag tcgcacccac tgcaggtgtt gcctgagcaa
     1681 gcggcgaata ggaacctgca cctgcactgt ttaccgcacg cactcggaaa tcataactga
     1741 gtgcgttagt gagccctgtt acggctgcgt ttgttgtagt cgatgttccg tctgaaaaag
     1801 ttgtccaaga actatcggag gtaagtttgt actgaatatt gtaatcagta attgctgaag
     1861 tccctgtata aaacggtgcg ctccagttta ccgtaacctg cccacttgca gtgctcgtac
     1921 tgctaataac tggcgctgcg gctggaccag cttctgtacc gtagatatcg atattgtcgt
     1981 accacacatc ggttgccggt ggaaatgatt ctaacgttgc aaacccgagt ccaaggtagc
     2041 tgccggtaaa gggcgaaccc gatgtatcag tataggtcaa tgccggagca ccgcgcgaac
     2101 ctgattttgg ataaatccaa atagtaatga caggctgatt tgacgcattg gttattattt
     2161 ctgcctcaac gcggtagatg gttccgttgg tcatcgtgaa cgattcatcg gcaacggttg
     2221 gtccggcacc accatcgccc gccgcatgtt tccagacaat tagctggtcc ccgcccgagc
     2281 caggccacaa gaaagtgtcg tacccctgat catttcctga accagtactg cgaccaaacc
     2341 acacctgcgg gaaaaaccga ccactctccg taaatcctga aaaatcaaac ccgatgtgtg
     2401 cattttgccc agcactttga tgaatagcaa acttggtaaa atctgccgat gacgttccgg
     2461 tatcgggcgc tactactttg ctgccgctgc gcgaatacac tttccacagc acgcttgctg
     2521 cggtgtcacc cggcagccag ccattttgtc cgcttacatc ggtacttgta acatatgact
     2581 caaagtcatc agtgatgatt tttttaagtg ggattgtggt gggattgttt gaatcagttg
     2641 ccgtgccatc gccgttgact gcctgtacgt taaattcgta gcgcgttcca ttcgtaagcc
     2701 cggtaacagt agtagttgtt acagtagaag taccgtcact aaatgttgtc catgaccccg
     2761 caccaatagt gcggtagcca atcacgtagt cagtaattgc tgagcctccg tttgcaggtg
     2821 ccgtccagct taaaaatgct gaagtatcac ccgagccagc agcgagcgtc gtaactgctg
     2881 aaggcgcagc agcatccacc ggaagacttt tgtacaatac aaaagaaaaa gcagtaagaa
     2941 cagccagaac aacagacact attttcttca tataattttt atattgtacc atcttctaaa
     3001 gcaacaaaaa aacgagtctt ttagctcgtt tttttgtctt agagaataaa ctctctacca
     3061 ttgtaaaaat ttatttatgc acaaaggtga gcgcagttcc ttttgaagat ccgcgaccag
     3121 tacgaccgat gcgatgaata tacgtttcgt aatcctgcgg cacgtcgtaa ttaataacgt
     3181 gcgtaatgtc agggatgtcg ataccgcgag cagcaacatc agttgccacc aacacctgta
     3241 cttcgccttt tgcgagtgag cgcaccgcga gttcgcgctg gcggttacgc atgtcaccgt
     3301 ggagcgcgag cgctttaaaa ccgcgatcac gaagggtgcg tcctaagtca tcaacatgac
     3361 gcttcatctc gcgaaagatg agtactttgg cacattccgg ttttatgagt aaatcgtgga
     3421 gcgcttcaat cttgtgctct ttactctcta cccgaacgac atcttgatca acgttctgtg
     3481 cagtatctcg agtttttact gaaatcgtta ccggttcacg caaaaattct ccgcagagtg
     3541 tttttactga aggcgacatc gttgccgaga agaacagact ctgccgttct ggtgccataa
     3601 gctttaagat gtagcgcatg tcggcgatga atcccatatc catcattcgg tcagcttcat
     3661 caagcacgat tgttttaaac tcgggcattt ttattttgcc gcgattgatt aaatctttca
     3721 ctcgcccggg ggtaccaatc acaaagtgca ccccgcgttc aagctcgcgc atctggctgc
     3781 caatcggcgc gccgcccaca caggtaacac tccacaattt aaaaccgcgc ttaaacatta
     3841 gcagctcttt ttcaatctgc tgcgcaagtt cgcgcgttgg cgcaagtacc atcaccttct
     3901 ggtgaggatt ctctgcaagc ttgtggacaa gcgggagtaa aaatgcagcc gttttaccgg
     3961 ttccggtatt tgcaataccg atcacatctt tcccctgcat cactaccgga attgcctggt
     4021 cttgaattgg tgaaggagta acatacttgc gtgctaccaa gtttgaaaca atagtttcgt
     4081 gcagcccaaa atcagcaaag gtgtgcgttg gggtatacac ctcaagaaca cactcttttg
     4141 ctttattaat gtagagcgtt tcgtctataa agactgtttt gcgtttattt cgagcaccgc
     4201 cgccgcctcg aaaaggagga cgtgagccgt taaaaggtcg tcgcggacca aactgtgtcc
     4261 gtgcgcgaga cggagcatac gaggcagtct tgctgcctcc gcgtatttca tttttataca
     4321 tatttattgt gtttcgttaa ccgagactat cattccgtgc gattctgtaa cgaaaacaca
     4381 atgatactca ggggatttgt aataacttct atagagtaat acaagacagc taaaaagtca
     4441 atagcgacta aaaatcccct actaatctac gagaaagcat cccttttgaa gcacctgtgc
     4501 tcatagagca taggaaaatg gcctttaaga ggccattttt tctttacaca tttaccaaca
     4561 tcccatcaat aaaatacgga ataggattat ctgccaaaaa ggaaagtaca gctccaggaa
     4621 cattcccgta cttttcccat ccgctaacgg caggggtaga aacattaaat aagtttccaa
     4681 agctttcctg atttagacca tgcacatatc tatagtgcaa tatcttttca ctaggagtat
     4741 ctggtttttc caacgggcaa tatccgaaaa atttaaaaat ccagcgaaaa tcctggaatc
     4801 gaacaccttt tccttgttcg atatcaatca aaactttaat attaatatca acctgtttcg
     4861 cacacatctc ttgggagatg cccaaggaaa ttcgcttttc gtagatcttt tcccccatat
     4921 ctttcagctg atcatagaat cgctggtagt caaatttttc gaccaccagc ttctttcttc
     4981 ttttcttttt tgaaccctgg gcctgctctt ttttagctac ttctgtagaa ggtatatctt
     5041 caagaagtaa cataattttt tccttgaggt ggttttgcgc ctcattaata ctctctgctt
     5101 tacagagcac atactcaaaa cgaaatgtat tcagtgcttt tacagtgact ttcctggcag
     5161 tagctggcac acagccgttc tgcatagcat aagaaacgag cggattaaag agttgggaaa
     5221 ataagtaatt tcctgcgatt ctatttccag agcgaaactc agtaaataat ttttcctcag
     5281 ttccaaattg attaggagtc atatatacac tttttaagta atgaataaat attttttagt
     5341 atttaaccac atattttaaa aaatgcaaat atcccgcttg aatcctctta cttaatttca
     5401 atcttctcac ctaagaattt tggctctttt ctaaaaaaga cgtcgagcac cgcttttacc
     5461 cgagcgaact tttctctaat ttttgttttg atatcagcat tcagataagc atcttgcgca
     5521 ttaaatccta tcacttctag atttctactc ctcgctatat aaatcgcccg ctcattatga
     5581 aacgcctggg aaattatagt aaaatttgtt tgtccaaaga ttttctccat ccgcactaca
     5641 gaatcatagg ttctaaagcc agcgtaatct aaatagagtt ttgattcagg aattccgcgc
     5701 gctacgagcg caattttcat atcttccggc tcgttgtact cttttctgct gttatcgcca
     5761 ctaattacca ggtactcaat ttttccagcc tggaacagct cgaccgctgc ctgaatcctg
     5821 tattcaaaat aaagattttg ctgccccgag gagagtgttt ttgcagtacc taggaggagc
     5881 ccggctttgt tatacggaat atcttgcatt tgtgtataca caaaatcttg tgccgtagat
     5941 tcaatctgtc tgtcagcaaa ccatgcaagc acgagcatta aaaaagctgc tagtgctagt
     6001 attctcaaaa gttttttatt cataactaca atatatatag attgtatcaa taaaaagaaa
     6061 agcctggtct tgcaccaggc ttttcttttt atgcgcgaac gacgggattt gaacccgcga
     6121 cctcccgcgt gacaggcggg tgctctaacc aattgagcta cgtccgcaaa tttaattgct
     6181 atagtgccga tgacaagatt tgcacttgtg accttctctt tatgagtgag acgctctaac
     6241 tacctgagct acaccggcaa cagaatatca ttctttaggg cttttatcaa tcctagtact
     6301 ttatatttta acgccgtact ttttcgaact tttaaacggc aaatcccgaa ataagtatca
     6361 tggttggcat ataccttctt tccctctgtt ttcacaaagc tcggattccc catttgttcg
     6421 ctagtcaatc ctagcagttt cttccaaaat ttcaatacat cattaatccg tggtttatgt
     6481 acctggttaa ttttaatagt acagaataat tcttgatgac taattccgtt agttaaaaga
     6541 aactttttaa tacaaagaat cattacaggg tcagaattta taaactgaaa cccagcagta
     6601 gaatcacttt ttgaaccttc agcccaatag agggcagtag ctataagcaa cagttctttt
     6661 ggggcaagtt ttttaattgt ctttttcccg taactatcgg cagcagctat ggcatcgagt
     6721 cttttttgtt tattcatgag cgcaccaagc attcttccct tactcgtttt tttaagagaa
     6781 tttttcaaga ttgccttttt ctgggtatca gaaagagtaa taccccgaca ccacatactt
     6841 gccgtacctt ttgaaacact gagtgtttga gcaataaccc caataggcat tccttttttg
     6901 cgtaaggtat gcgcttgcat acgtttttga tataaagcca tatatgcatt atatcatctg
     6961 ttctatttca agaacgagca acgagctgct ctttcttcgc tcgcggccag cgttttattt
     7021 cagcttcgcg tttttgtgcg gtactgcgcg tgtggaatgg ttcagagtaa acacaggaaa
     7081 caggtttatg acttcttgtg tattttgcac ccgtaccctg tacgtgctgt aaaaaacgcc
     7141 gctctatatt gttggttaca ccaacataat agctttcatc tgcacacact aaaatataca
     7201 tgtaccacat aggcagcgag cgcagcgcgt cgaaatgttg cggggcccgg aatcgaaccg
     7261 gggtcttaag gttatgagcc ttacgagatg cctcttctct accccgctct gcaacaaaaa
     7321 atactgtact acgcacctac aatttatgca acatcaataa caccgagctc tttgaatact
     7381 gcctcaaaat tcgccagcgc ccggcggact tcaggaagag tgaaagtaaa gtcagcaccc
     7441 tcgtgcgcaa tccgattgcg gatcttgtgc gcctcccacg caagctgcac gctcgcgatt
     7501 ttttcaggtc caatctgctt gagcttatct gagagtgtat ccccctggta tcccttttta
     7561 gttaaggttc ggtcaagcaa aatatcggct tcaataatac ccaagcgcca ctcactttca
     7621 tcatccgaga gcatgcgccg gcgcatcgct tcccatgcct cattttttgc tactggcact
     7681 gcagtgcttg cagcaagcag tttttcagcg gcatccgctg cggtcttttt ttgctgctct
     7741 ttttttattt catgcagtcg aatgaggcag tacaaaattg cagtaatcaa aagaataaca
     7801 atgatagtcg caagcacctt caaatcattc caaaatgcac cagtcaggat gtaatcaatc
     7861 actccgtgaa aagtccggta gagcaagaaa aaaacgtatt cgaggtttaa gaataccggt
     7921 tccggaatgt cggggccgta catgctctat agtataccct atcgtatcga ttcaattatc
     7981 tttcctaact caaaaagtgt gtgctcacca aagcgcggac caatgcattg gaatccgagc
     8041 ggaagccctg cattagtttt accgtttgga acagaaagtg caggcacttg cgcaatattt
     8101 gcgggcactg taaataaatc agagagatac atggcaactg gatcatgtgc tttttctcca
     8161 atcttaaacg cgggtgttgg agttgttgga gtgataatcg catcataggt tgtacatgcc
     8221 ccctcgagct cggtgcgaat aagtgcacga agtgcaactg ccttgttgta gtacgcatcg
     8281 tagtacccat gcgagagtac gtaggtgccg agtaaaattc ttcggcgaac ttcctttcca
     8341 aacagctgcc cgcgcgtttg tttatacatt tctgaaactg aggcatcact catgcggtcg
     8401 ccgtagcgaa caccgtcaag acgagaaagg tttgatgaca cttctgccgg ctgaataatg
     8461 tagtacaccg caagtgcatc gacaaaatgc gggagctcga tttcatcaat cacaaatcct
     8521 tcctttttta atttttcaat agtcgtttca aaattttctt gcacctcttt atcgcacccg
     8581 ccgttttcaa aaattccttt tggaatggca agccgctttt taagcaccgg ggtagtttca
     8641 gtacgagcaa ctgaggtgct atcgagcggc tcgggtacgg caactgcttt atacaaaaac
     8701 tctacgtctt caaccgtgcc tgcaaacgga ccaatcacat caagcgatga cgccattgca
     8761 atcagcccat gacgcgaaac agtaccgtat gtaggaagaa gccctgccac accgcaaaat
     8821 gctgccggct ggcgaatcga gccgcccgtg tcgctcccaa atgcagcaac tactaaattg
     8881 gctgccactg ctgcagccga accgcccgat gaaccgcccg gtacccgtgt ttcgtcgtgc
     8941 gggtttttgg taactccata ggctgagttt tcggttgatg aacccatcgc accctcgtcc
     9001 atattagtac gcccaataat caccgcgcct gcttttttaa gttctcgcac caccgttgaa
     9061 tcatacgcgg cagtgtagtg ctcgagcata tgtgaacacg cacctgcaat gtgccccgca
     9121 aacataatgt tgtctttaat cgcaaccgga atgccggtga gcaatgttgc tgtgccgttt
     9181 gcaagcattg cttctgcttt ttgtacctgt tcgtcaatat ccgaaaaaat ttctcgaaag
     9241 cagttaagtg tgtccgcttt tgcacgcgca acgtactcgc caactagttc agtaatcttc
     9301 acctctcctg aaactatttt tttgtggagc tctagaatag ttaccatatt actcaattac
     9361 cttcggtact tttacaaaac catcctggtg caagggcgcc gctgcaacca agtcatacac
     9421 gctttcaagt ggtgcaactt catcttcacg aaacacgttt acactgttgt accctgccgc
     9481 agcagaaaca tcccgagact gtacctggtc aacaaagccg ataatggttt ctaggtcttt
     9541 cgctaccgcc tcctgctcat ctttgggcac ctcaagccga gccaagtctg ccagtttttg
     9601 aacgtctatc atagggaaaa tatagcagat ataaggtttt tttcaaaaat tgacaattaa
     9661 cataattaaa actaagctct agtaaatttc aaaacaattt aaataactct tatgacaaaa
     9721 acactcgtag agcttcagga atcagaaact tttaccaatg ccgacctcat cgaaacaatg
     9781 aaacattcag tagaaaataa actggaaata aatcccagcc ttgtcatgca cctaatcaat
     9841 cgtgtagaac ttggagatat agaacgaaac actatgtggg agctcgcaaa aaaagggcgt
     9901 gatgagcaaa tcgataagcg taacaaaaaa gcagcagatg gttacgaaat gattatgggt
     9961 tatttacatg aataagacaa aatattcaca agatctatcc cactgctagc agtgggattt
    10021 tttatttcac cattttcaaa aattttcctt catcaaggac aggtattccg tgcttttgag
    10081 cttcagtgag tttgctgcca ggattctcgc ctgctactac atagctggtt gcttttgaga
    10141 cactgcttga aacgttgccg ccgcgctcga gaattttttg cttcgcttct tcacgtgaga
    10201 gtgtttcaag ggtaccggtg agtacaaacg ttttccccac aaacggtttt ggctttttat
    10261 catcttttac tggcaggaca accacgccct gtttaaataa tttttctata aatcgctttg
    10321 cttccggtcg tcttcgatac tcggcaatac ttttaataac cgccgggcca atgttcggaa
    10381 ttgcatcgag tgcttccgcg ggggcgttcc agaacttctc aaacgtttta cattcattcg
    10441 ctaaatctcg cgcagtttcc tcgcctacgt gcagaatgcc gagcgcgtaa atgaaccgcg
    10501 cgagctgcac ctgctttttc ccctgaattg aactaatgat attttcagca ctcttctcgc
    10561 caaagcgttc gagtccagaa aggtctgatt cttctaaggt gaacaaatca gcggcatcgg
    10621 taattaagcc ttcatcctgc agtcggtcga taattttagg accgacggta taaatctcaa
    10681 acacatttac aaagtgaatg agcccgcggc ggttttttgc cggacacttg gggttggtgc
    10741 agtagtatgc tacccctttt ccggtttcac tctcacgttt cgccacctcc ccctcgcact
    10801 cggggcactt ttttggcatc gaaaactttt tttcttttcc ggtacgcagt tcggtgagta
    10861 cctgcactac ttcaggaatt acatcccctg ctttttgaat cactaccgta tcgccgacac
    10921 gaatgcctaa gcgctcgatt tgatccatat tatgaagtgt tgccttcgag accgttgaac
    10981 cagcaacaag cgttggttta aagtgcgcaa gcggggtgag cacgcccgtg cgaccaacct
    11041 gagtggtgat agcggttacg gttgttgttg cccgctcggc tggatactta aaagcaaccg
    11101 catagcgcgg cgccttcccc accaccccaa gccgatcgtg gagcgagagc tcgtcaactt
    11161 gcacgacgat gccatcaata tggtacggca gctgatcacg ggctccgcta atagcggcaa
    11221 taaaggtttc gatttccgga agcgtttttg caacacactc ttggctcgtt accggaaatc
    11281 cgagctcacg caaaagtgca tgctcttctg aatgaaactc aggaattttc tgagcaccgt
    11341 gcagtgtagc aatatcgtaa gcaaaaaaag agagctgacg actctgcgcg acactcggat
    11401 caagctggcg caccgagccg gcggcggcat tgcgcgtgtt ggcaaatttg ggcagccctt
    11461 tttcttcctg ttctttattt aaccgttcaa gcaccgactt ctgcatcacc acttccccgc
    11521 gcacttcaat atgctgtggg tagggcggca acagccgcag tgggatatca ctaattactt
    11581 ttatgttctc ggta
//
LOCUS       DATIBQ010000039         3115 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U16294
            k141_1266305, whole genome shotgun sequence.
ACCESSION   DATIBQ010000039 DATIBQ010000000
VERSION     DATIBQ010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935789
            Sequence Read Archive: SRR25158331
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:58:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 637
            CDSs (total)                      :: 603
            Genes (coding)                    :: 601
            CDSs (with protein)               :: 601
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3115
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1266305"
                     /isolate="SMAG_U16294"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            140..1483
                     /gene="secD"
                     /locus_tag="VLB02_01155"
     CDS             140..1483
                     /gene="secD"
                     /locus_tag="VLB02_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HSE56674.1"
                     /translation="MHRIMLKRRIGALLVLIIAVAIGWFVYASQVKTAGTLSKYPFRL
                     GLDLSGGTHLVYKADISQLPAGQVNDSMDALRDVIERRVNLFGVSEPVVQVQHGNLAN
                     GQEERLTVDLPGVTEVAAAVKMIGQTPFLEFRTQSKDVQKAEVGADGVVTVDPTGNFV
                     PTQLNGKYLKNAVLQFDPNTMEPTVVLQFNDEGAKLFETLTKEHVGEVVAIFLDGEPI
                     SMPVVQQAISGGEAVISGNFNAQEAKQLVGRLNSGALPVPISLISTQTIGASLGSEAV
                     AAGMRAALVGFLIIALFLIIWYRLPGLIAVLGLAIYVAITLTLYKLIPVTLTAAGIAG
                     FIISIGLAVDANILVFERFKEELRAGRTIADAMSSGFSRAWFSIRDANTSTFITAVIL
                     FWFGTSLIKGFALTLGLGVLVALFSAITISRILLRALPQGNSKILHFFFSSGISK"
     gene            1496..2380
                     /gene="secF"
                     /locus_tag="VLB02_01160"
     CDS             1496..2380
                     /gene="secF"
                     /locus_tag="VLB02_01160"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HSE56675.1"
                     /translation="MKYKKFFVLLSVALVAASITLLCIFGLRLGIDFKGGAQMELSYT
                     GERPEMAVLKSAAANAGFGDALVQPAGEHDYIIKTRDLSEAERTALIAAATINGTFPA
                     EQKSFTSIGPSVGSELKRKAIGAIILVLIAIITFIAYAFRKVSKPVSSWKYGLIAVVT
                     LVHDVVIPMGIYVVLSWVYGTEIDTLFVVALLTILGLSVADTIVVFDRVRENLHAKKG
                     QPFETLVGESLRQTFVRSINTSLTVILAVIALAVFGPESTRSFAIVLAAGMFFGAYSS
                     IFLASPLLVYVNQWQGKK"
     gene            2512..>3115
                     /locus_tag="VLB02_01165"
     CDS             2512..>3115
                     /locus_tag="VLB02_01165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56676.1"
                     /translation="MNAWVSFFGRNTLFKKTVSGLATLSLLLGMLMPIIGSGKVFAVD
                     EPLLPTVTASVNTPSITLPVDYVQLQSEAHNNFWGEWSFVNGPDGVTPDITHPTDAYS
                     DVAGLVEPGTYTFKVTVSDFSKEHSAEATVDVVVNEAAVEPDDTEDEDLVVNEVDLVP
                     SNDLKGEIRICKVTLDAEGNVAASATGTFTLESIGDATDHF"
BASE COUNT          765 a          653 c          783 g          914 t
ORIGIN      
        1 tatttatgtg ggagtgcact atccgtttga tgtcctcgct gggttctgta ttggcttttt
       61 agtgccgtat acgatccggc gtattatacg ttggagtacc gagcgctaat acattgcgag
      121 aaaaagtaac catgatagaa tgcaccgtat tatgctgaaa cgaagaatcg gggcactgct
      181 agtgctcatt attgcggttg ccattggttg gtttgtctat gcctcgcaag taaaaacggc
      241 gggcacactc tcaaaatatc cctttcggct tgggttagat ctttcggggg gaacacactt
      301 agtatacaag gctgatatct cgcaacttcc cgcggggcaa gtgaatgatt ccatggatgc
      361 gctccgcgat gtgattgagc gtcgcgtaaa cttgtttggt gtttcagaac cggtagtgca
      421 ggtgcagcac ggtaaccttg caaatgggca agaggagcgg ctcacggttg accttccagg
      481 ggttactgaa gttgcagcag cagtgaagat gattgggcag acaccgtttc ttgaatttcg
      541 tactcaatca aaagatgtgc agaaggcaga agtgggagct gatggcgtgg taacagtaga
      601 tccaacgggg aactttgtac cgacccagct taatggtaag tatttaaaaa atgcagtctt
      661 gcagtttgat ccaaatacca tggagccgac ggtggtactg cagtttaatg atgaaggcgc
      721 aaaacttttt gagacactca ctaaagagca tgtgggagag gtagtagcaa tattcctcga
      781 cggtgaacca atttcaatgc cggtagtgca gcaggcaatt tctggcggcg aggcagttat
      841 ctcggggaac ttcaatgcgc aagaggcaaa gcagctcgtg ggacgactta actcaggagc
      901 actaccggta cctatttcgc ttatctcaac acaaacaatt ggtgcatcgc tcgggagtga
      961 agcagttgcc gccggtatgc gagcagcact cgttggtttt cttatcattg ccctcttttt
     1021 gattatctgg tatcgcttgc cggggctcat tgccgtgctt gggcttgcta tatatgttgc
     1081 cattacactt actctctaca agctcattcc ggtaacgctc accgctgccg gtattgcagg
     1141 gtttattatt tctatcgggc ttgcagtgga cgcgaacatt ttagtatttg aacgattcaa
     1201 agaagagctc cgcgctgggc gcaccattgc cgatgcgatg agttcagggt ttagccgtgc
     1261 ctggttttca atccgtgatg caaatacaag cacctttatt actgccgtca tcctcttctg
     1321 gtttggcacc tcgctcatta aggggtttgc acttaccctt gggttggggg tactggtagc
     1381 gctcttctct gcaatcacta tttctcggat actcttgcgt gcgctcccgc agggaaatag
     1441 taaaatacta catttctttt ttagtagcgg aatctcgaaa taatatatgt ttataatgaa
     1501 atataaaaaa ttctttgttc tcctctcggt agcactcgtc gctgcttcaa ttacactctt
     1561 gtgtatcttt ggtttgcgcc tcgggatcga ctttaagggc ggtgcacaga tggagctcag
     1621 ctatacaggc gaacgccccg agatggcagt actcaaaagt gcagctgcaa acgctgggtt
     1681 tggcgacgca ctcgtgcagc cagccggcga gcacgactac attattaaaa cgcgcgatct
     1741 ttctgaagca gaacgtacag ctttaattgc tgctgcaacg ataaatggca cctttccggc
     1801 tgaacaaaaa agctttactt caattggtcc gtcagtgggg agcgagctca agcgaaaagc
     1861 aattggcgct attattttgg tactcatcgc aatcatcact tttattgctt acgcatttcg
     1921 gaaagtgtca aaaccagtgt cgtcgtggaa gtacgggctg attgcggtag taacactggt
     1981 gcatgatgtg gtgattccga tgggaatcta tgtagtgctt agctgggtat atggtaccga
     2041 aatcgacaca ctgtttgtag tggcgcttct taccatcctc ggtctttcag ttgccgacac
     2101 cattgtcgta tttgatcgtg ttcgcgaaaa tttacacgca aaaaaagggc agccgtttga
     2161 aaccttggta ggggagagtc ttcggcaaac atttgtgcga tcgatcaata cctcgctcac
     2221 cgttattctt gctgttattg cactggcggt gtttggtcca gaatcaactc gtagctttgc
     2281 tatcgtgctt gctgccggca tgttctttgg tgcctactcc tcaattttct tggcgagccc
     2341 gctcttggtg tatgtaaatc agtggcaggg aaaaaagtaa ccccgcatcg ttttattgac
     2401 atcgcaaacc agagtggtac tattatctta gattgaattt ccgagcgtag tcgctcggag
     2461 ttgttcggtt gcttttgttt tttattagta attttttatt gagtaaattt tatgaatgca
     2521 tgggtatctt tttttggtag aaacaccttg tttaaaaaaa cagtgagcgg tcttgcgaca
     2581 ctctcactgc tcctcggtat gctgatgccg attataggtt caggaaaagt gtttgcagtt
     2641 gatgagccat tactccctac tgttactgct tcagtaaata ctccatcgat tacacttcca
     2701 gttgattatg ttcaactcca aagtgaggca cataataatt tttggggtga atggagtttt
     2761 gtgaatggtc cagacggggt tactccggat attactcatc ctactgatgc ctacagtgat
     2821 gtcgctgggc tggttgaacc tggaacatat acatttaaag tgactgtttc tgatttttct
     2881 aaagagcatt cggcagaagc tactgttgat gtggtagtaa atgaagcagc tgttgaacca
     2941 gatgataccg aagatgaaga tttggtagta aacgaagttg atcttgttcc aagtaatgac
     3001 ttaaagggag aaataagaat ttgtaaggta acgcttgatg cagaaggcaa cgtggcagca
     3061 agtgcaactg gtacttttac tcttgaaagt attggcgatg caactgacca cttcg
//
LOCUS       DATIBQ010000040         9992 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U16294
            k141_1889763, whole genome shotgun sequence.
ACCESSION   DATIBQ010000040 DATIBQ010000000
VERSION     DATIBQ010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935789
            Sequence Read Archive: SRR25158331
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 9992)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9992)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:58:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 637
            CDSs (total)                      :: 603
            Genes (coding)                    :: 601
            CDSs (with protein)               :: 601
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9992
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1889763"
                     /isolate="SMAG_U16294"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(114..1766)
                     /locus_tag="VLB02_01170"
     CDS             complement(114..1766)
                     /locus_tag="VLB02_01170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56677.1"
                     /translation="MYDNSGNAIAVSTDSNTVRVVSNTLVSAGKVSLPQPVSSDDNRI
                     RYHDTLYGKDYIGLFRTSDNGTNSNRFMVLNANSGKYEPVSTLPLPSTNSVDGFFDLG
                     DSLVLLTGNLIYHVSSTYYDNAAVWNMRTNTLSRPCATEWFVCSNGSGGEQKQSVSSV
                     DGTAMFISPAGSSFSVVWKGQNYWQGINYPTVPSGGIFTACQVIDKNIIYAAWSKDPA
                     TVLTPAHLMKFSGGAWESVIKFTRDAAPDFDDGAYADRCNINSIGQLGNLLYIAHTGD
                     SANGVKVSNIVTFNMTNGEVKSVAEIPNGWSSVIYKTGKKLFQKSWVKILNAEVCLFE
                     TTFEALSHQEKQTVYILRDTTDVTKPAALTIVEPANAAVALRQQTVTFKATGAENNSK
                     ALFHFVFIGSGTANRTEWTDTVFVINTAQGVAALQRAFDQPGTCYWTVTPVDQAGNIG
                     QESSERTLTISAVTGIGDVTNTSVIPSLYPNPVQGATQVIITNVKRVLHAVTVNGQRI
                     PITFIAKESSVVLDVSRLIPGFYGIVCEVSKGKFITKPMIKQ"
     gene            2229..3473
                     /gene="nusA"
                     /locus_tag="VLB02_01175"
     CDS             2229..3473
                     /gene="nusA"
                     /locus_tag="VLB02_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HSE56678.1"
                     /translation="MLDVKALKIAMETLESERRIPREKLLDAIEQSLAAAYKKEYGKK
                     GQIIRSKIDFDTGSVDFYQVKIVVDDSVVRFVAEGEEDVVADENDVRERFNEEKHIVL
                     DDARIIKQGAEVNDEIVFALEAKDDFGRIAAQTAKQVIMQRIREAERSSIIDEFGTRE
                     GDIVSGHIQKVERGTVFVDFNRAVGMLPPNEQIQSERYNRGSRIRAYLYSVEEGPRGV
                     TLRLSRTHPRFLERLFAYESPEVASGVVQIKAIAREPGSRSKIAVYTEDQNIDPVGSC
                     VGQKGVRVSTVMNELSGEKIDIIEWSADPAEFVRKALSPAKVLDLHLNHEERKATIEV
                     TPDQLSLAIGKGGQNVRLAAKLTGWKIDIKGSEAEAETEVAEGFPNEGDDFVSLADLK
                     KETEAPAAEPEEELAPEPKERE"
     gene            3495..4025
                     /locus_tag="VLB02_01180"
     CDS             3495..4025
                     /locus_tag="VLB02_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012189261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HSE56679.1"
                     /translation="MNLLHDISAGKNVPESLNCIIEIPKGSKNKYEIDKETGMITLDR
                     AMHTSQDYPFDYGFVPQSHWEDGDPLDVVVLTTYPLAPGILVKVRPVGVMHMIDDGES
                     DAKIIAVPEKDPRWNEVHDLKDVNKHTLKEIQHFFSTYKNLQEKEVNINGFGDAAAAK
                     EAILKSLQLYKETFGK"
     gene            4249..5010
                     /locus_tag="VLB02_01185"
     CDS             4249..5010
                     /locus_tag="VLB02_01185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56680.1"
                     /translation="MKRTIITSAVALSLLLGTAAFAQNTATTPPMPPAPSVNKARLGE
                     LKDEMQAKREALKTDMQERRTDLKEDAEAAKKALQEKRAALAEELKAKRAALQAELKT
                     KREALKAEIAKVKKERVIVRKGYVEQKLRQVLVVLTEKQARIQKAIDALKAKGNDVTE
                     AQKHLDESKAALKTATEKLDALVKLVINENEPKPLEAARALAKEAEAALKSTREHLVL
                     AIQELKLLTSDDEDDSDDDTNETTSTTGTTTNTQN"
     gene            5013..5309
                     /locus_tag="VLB02_01190"
     CDS             5013..5309
                     /locus_tag="VLB02_01190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56681.1"
                     /translation="MDNTMLNQTQTEQMPQNGAPMGGKPEHKGGKGIFIAILIIVVAA
                     LLGYWYLNAPKQVPEVQNVQQPAGTRATTDASAEVSAAEDGLDNMYFEGVSDGL"
     gene            5390..6445
                     /locus_tag="VLB02_01195"
     CDS             5390..6445
                     /locus_tag="VLB02_01195"
                     /inference="COORDINATES: protein motif:HMM:NF017508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HSE56682.1"
                     /translation="MTHTKKYTLIITNLPGGEIELAGNLPWDTFLTYEAAALAHLGKN
                     IEVDGFRKGSVPETMVRKHIPDQLLLEEMAERALQEHYVQMLTQESIDAIGRPRIALT
                     KLARDNDLEFKIITAVMPEVKLPDYHALAKAAEKIEAPAVTDEDVENTINELRHMRLH
                     QNDTAGTHDHSTPHEHTEAELPVVDDAFAKSFGKFETVADLKAKIRENLALEKDREAK
                     DKQRTAVLEAVSKETEVVVPKLFVEAELQKLVSQVEFEVSRAGVKFEDYLKQVGKTKE
                     DLEKEFLPDAERRAKFQLVLDAIANKENLKPEAEVVEQETQKLMTMYPGADEARTRAY
                     AQMVLTNEKVLSFLETL"
     gene            complement(6508..7065)
                     /locus_tag="VLB02_01200"
     CDS             complement(6508..7065)
                     /locus_tag="VLB02_01200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56683.1"
                     /translation="MLFIKNFIRLPWFMWKACIGFSHICTTEITDKTVWGDVYYQISL
                     TASAVILFTGAGFGIFYLLYPTTPLSIVFQILFETIPVWGWIIIGWKIISLLHHAVQN
                     SRLRFDHVGELILIPKVLHLLELVPLGILHIWSYKKTGLAYKAVQAKIRDTKEFFRIH
                     WYWSIDTPLWKNRLPERVKKYLFGN"
     gene            7226..7833
                     /locus_tag="VLB02_01205"
                     /pseudo
     CDS             7226..7833
                     /locus_tag="VLB02_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007502498.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
     gene            7844..9364
                     /gene="rny"
                     /locus_tag="VLB02_01210"
     CDS             7844..9364
                     /gene="rny"
                     /locus_tag="VLB02_01210"
                     /inference="COORDINATES: protein motif:HMM:TIGR03319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HSE56684.1"
                     /translation="MNTLPILFGTLLAGALLGYWLRMLIALSRKGSIELETKHRLVET
                     KEAAQRIIDTANKKAEERLAELRTEEHTREEKLQNTETRLIKKEELLDTRAAELDREH
                     ETLKEREGTVLARESAVQNDEALLTERLEVIGKLTMSEARAALIARTEAEFAEDLELH
                     RHKLETQMQEDIQLRAREILATAINRLAVSVASELTTATIQIPSEDIKGKIIGKEGRN
                     IRAFERLAGVELIVDDAPGAIIVSSFDPVRRAVAAEALGELIKDGRIQPAKIEEVIQK
                     AQENSNETMKKRGTEAVYECGIYNFDPRLVSVIGRLHFRTSYGQNVLQHSIECAHLAG
                     MLAEELKADVQVAKAGALVHDIGKALDHEVQGSHVDIGIRILRRFNTDERIIAAMKSH
                     HDDCPHETVEAVIVQVADMISGSRPGARRDTAENYIKRLQELEGLAVRFAGVERAFAL
                     QAGREIRIFVHPENVTDSEARTMARAIALEIESELRYPGEIKVTIIRETRIIDFAR"
     gene            9383..>9992
                     /locus_tag="VLB02_01215"
     CDS             9383..>9992
                     /locus_tag="VLB02_01215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56685.1"
                     /translation="MHEQPPKPDSSEEKNSAPKEGGLLKKIGLAAGMMAAGTAALGSE
                     GKTAEKELINPDHNIEAAAKTDTMDHKENTASFESGRRGGLEITEGQELFGLKVEGKP
                     VPVSSLDTTKQFFFIKFSAVNKKDNANVGKILQAMDSLHIQPGNSEHLKSVFLSNPKV
                     SKQIGGYGMAIGDKVSDGNTGRATINPVGMKMMEDRASTELDI"
BASE COUNT         3096 a         2307 c         2248 g         2341 t
ORIGIN      
        1 atatcgcacc tcaattttga aagacaatag agtgtgtgga tagaagaaat atgtcaaaca
       61 aaaaagccta accgtcatta cacagctaag cttttgttaa caaaaaatta aatttactgc
      121 ttaatcattg gttttgttat gaactttcct tttgaaactt cacatacaat cccataaaat
      181 ccaggaataa gccgtgagac atcaagcact acagatgact cttttgctat aaaagtaata
      241 ggaattcgct gtccatttac tgttactgca tgcagaacac gctttacatt ggtgattata
      301 acctgagtcg ctccctgtac cggatttggg tacaaagagg gaattacgct tgtattggta
      361 acatcaccaa taccagtaac tgcagatatg gtcagagtgc gttctgagct ttcttgcccg
      421 atgttaccag cttggtccac cggagttacc gtccaatagc aggtgcctgg ttgatcaaac
      481 gctcgctgga gcgctgcaac tccttgtgca gtatttataa caaacactgt atccgtccat
      541 tcagtacggt tggcagttcc gctgcctata aatacaaaat ggaacagcgc tttggagtta
      601 ttttcagcac cggtggcttt aaatgtaact gtctgttgtc gcagcgccac cgcagcattg
      661 gctggttcta caatagtaag cgctgctggc ttagttacat cagtagtatc ccggagaata
      721 tagactgttt gcttttcctg atgactcagt gcttcaaagg tggtttcaaa taaacacacc
      781 tctgcgttga gtatttttac ccaacttttt tgaaaaagct tttttccggt cttataaatg
      841 acggacgacc acccgttagg aatttcagca acactcttca cttctccatt cgtcatgttg
      901 aaagttacaa tgttgcttac ttttactcca ttagcactat caccagtgtg cgcaatgtag
      961 agcagatttc ccagctgacc gatactattg atattgcacc gatcagcata cgcaccatca
     1021 tcaaaatctg gggcggcgtc tctggtaaat ttaattactg attcccatgc cccaccactg
     1081 aatttcatca agtgtgccgg agttaaaaca gtagcaggat ctttgctcca agcagcataa
     1141 ataatatttt tatctataac ctgacaagcg gtaaaaatac ctcctgaagg aacagttgga
     1201 taattaatcc cctgccaata attctgccct ttccatacaa cactaaatga agagccggct
     1261 ggacttataa acatagcggt tccatcaacc gatgaaacac tctgtttctg ttctccgcca
     1321 ctaccgttac tacaaacaaa ccattctgta gcacacggcc ttgataaggt gttcgttctc
     1381 atgttccaaa ccgcagcatt atcgtagtac gtactggata catgataaat caaatttccg
     1441 gtaagtaaga cgagtgaatc gccaaggtcg aagaagccat ctaccgaatt agtactcggc
     1501 aaaggcagtg ttgaaacggg ttcatacttt ccactgttgg catttaatac cataaaccta
     1561 ttagagtttg tcccattatc acttgtccgg aaaagtccaa tgtagtcttt cccgtacagt
     1621 gtgtcatggt atctaatccg attatcatcg gaacttaccg gttgaggaag ggatactttc
     1681 cctgcactca ccagcgtgtt actgacaaca cgcacagtgt ttgaatcagt agaaactgca
     1741 attgcgttcc cgctattatc gtacaacgtt gccttatcaa agtaaccgta gaaggatttg
     1801 tatcctccct ggttctttgt gtccgcgtgt tgtacaaacg aaggatagga actcgcttga
     1861 aaaaattttt tttcaaattc caaccactta acccacacag gctgagcggt tacctgttgc
     1921 gctagggcta tcaccatgac gcccatcagc gcgagaagag tagtagtcct cattggtgcc
     1981 tcctttttta attttttgtt tgttaaagaa atatttttaa gatcaaaatt gatattgaac
     2041 tagaataaat atatcgagtt attttgactg gatttaatca tgaaacacaa taaaagtcaa
     2101 tagcaattgt ggataaataa cgccctgatt ttagaaggat ttttgcaccg gattgctgta
     2161 tatgctacaa tgccctcgtt agttcccgaa cgcccgttcg ggctttattg ttaaaagaga
     2221 gtaaccttat gttggacgta aaagcactaa aaattgcaat ggaaaccttg gaatccgagc
     2281 gccggattcc gcgtgaaaaa ctgctcgacg cgattgagca gtcgctcgct gctgcgtata
     2341 aaaaagagta cggcaaaaaa ggtcagatta ttcgctcaaa aattgatttt gacactggtt
     2401 cggttgattt ttaccaggta aaaattgtgg ttgacgactc ggtggtacgc tttgtcgctg
     2461 agggtgaaga agacgtggtt gccgacgaaa acgacgttcg cgagcgcttt aatgaagaaa
     2521 agcacattgt gctcgatgat gcccgcatta ttaaacaagg cgcagaagta aacgacgaaa
     2581 tcgtatttgc ccttgaagca aaagacgatt ttggcagaat tgccgcccag accgctaagc
     2641 aggtaattat gcagcgcatc cgtgaagcag aacggagctc gattattgat gaatttggca
     2701 cccgcgaggg cgatattgtc tcggggcaca tccagaaagt ggaacgcggt accgtatttg
     2761 ttgattttaa ccgcgctgtc ggcatgctcc ctccaaacga gcagatccag agcgagcgct
     2821 acaaccgcgg ctcacgcatt cgcgcatacc tctactcagt agaagaagga ccacgcgggg
     2881 taacgctacg cctctcgcgc acccacccgc gctttttgga gcgtctcttt gcttatgaat
     2941 cgccagaagt agcctcaggc gtggtgcaga ttaaggcaat cgcccgcgaa ccaggcagcc
     3001 gttcaaagat tgccgtgtat accgaagacc aaaacatcga cccagtcggc tcatgtgtgg
     3061 gtcaaaaagg cgtgcgggtg agtaccgtga tgaacgaact ctcgggagaa aaaatagata
     3121 ttattgaatg gtcagcagac ccggctgagt ttgtccgcaa agcgctctcg ccagcaaaag
     3181 tgcttgattt gcacttaaac catgaggaac gcaaggcaac aattgaagta acccccgacc
     3241 aattatcact tgctattgga aaaggcgggc aaaatgtgcg cctcgccgca aagctcaccg
     3301 gttggaaaat tgatattaaa ggcagtgaag cagaggcaga aaccgaagtg gcagaaggat
     3361 tcccaaacga gggcgatgac ttcgtatcac tcgctgacct gaaaaaagaa actgaagctc
     3421 ccgcagccga gcctgaagaa gagctcgctc cagaaccaaa agaacgagaa taaaaactac
     3481 gttatactat atatatgaac ttacttcacg atattagtgc tgggaaaaac gttcctgaat
     3541 cgctcaactg cattatcgaa atcccaaaag gctcaaaaaa caagtacgaa atagacaaag
     3601 agacggggat gatcacgctc gaccgtgcga tgcacacctc gcaagattac ccctttgact
     3661 acggttttgt accgcagagc cactgggaag acggcgaccc gctcgatgta gtcgtcctta
     3721 ccacctatcc gctcgcacct ggcattttgg taaaagtgcg cccggttggc gtcatgcaca
     3781 tgattgacga cggcgagagt gatgcaaaaa tcatcgccgt acctgaaaaa gacccccgct
     3841 ggaacgaggt gcacgacctg aaagatgtaa acaagcacac tcttaaagaa atccagcact
     3901 tcttctcaac ctacaaaaat ctgcaagaaa aagaggtaaa cattaatggt tttggcgatg
     3961 ccgcagctgc caaagaagca attctaaaat cactccagct ttataaagaa acatttggaa
     4021 aataagacta gagaatagaa aaaccgcccg ggtacattat gtaagggcgg ttttcttatt
     4081 gagagttgca gaaactctta gggaattaag gataaaaacg ggctcttgtt tgagattaac
     4141 ataatatagt aattttgtca agcgttatcc cccgtgcttt ttttgctttt agattatact
     4201 aggggtattg gccacattaa tgattaacca atagtaaaaa ataacattat gaaacgaacc
     4261 attatcacca gtgcagttgc actctccctc ttgttgggga cagcggcgtt tgcacaaaat
     4321 accgcaacta ccccgccaat gccgccggca ccgagtgtta acaaagctcg gcttggggaa
     4381 ttaaaagatg aaatgcaggc aaagcgcgag gcattgaaaa cggacatgca ggagcgccga
     4441 actgacttaa aagaagacgc tgaagcagct aaaaaggcat tgcaagagaa gcgtgctgca
     4501 cttgcagaag agttaaaagc aaagcgcgca gcgctccagg cagagctcaa aaccaaacgt
     4561 gaagcactga aagcagaaat tgcaaaagta aagaaggagc gcgtgatcgt ccgcaaaggc
     4621 tatgtagagc agaaactccg ccaggtgctc gtggtactta ctgaaaagca ggcacgcatt
     4681 caaaaggcaa ttgatgcatt gaaagcaaaa ggaaatgatg taaccgaagc gcagaagcac
     4741 ctcgatgaat caaaagcagc gctgaaaact gctaccgaaa aacttgatgc gctcgtaaaa
     4801 ctcgtgataa atgagaacga gccaaagccg ctcgaagcag cacgtgcttt agcaaaagaa
     4861 gcagaggcag cgctcaagag cactcgcgag cacttggtgt tagcaattca agaactgaag
     4921 ctcctcacca gcgatgacga ggatgattca gacgatgaca ccaacgaaac gacgtcgact
     4981 actggcacaa caactaacac tcaaaactaa ccatggataa cacaatgctc aaccaaacac
     5041 agacagaaca aatgccccag aatggtgctc cgatgggagg caagccagaa cacaaaggcg
     5101 gcaaaggaat ttttattgcc atcctcatta tcgttgtcgc tgcactgctc ggctactggt
     5161 acttaaatgc tccaaagcaa gttccggaag tacagaatgt acagcagcca gcaggtactc
     5221 gggcaacgac cgatgcgagt gcagaagtca gcgctgcgga agacggactc gacaacatgt
     5281 acttcgaggg cgtttcagac ggactctaaa ctgcgttttt ctcaaaaaac caggctcatc
     5341 caagtctggt tttttgtttt aaaaaagctg tgttagacta ggcaatctta tgacacacac
     5401 taaaaaatat actcttatta ttactaatct tcccggcgga gaaatcgagc tcgccggcaa
     5461 tcttccgtgg gatacttttc ttacgtacga agcagcagca ctcgcccacc tcggcaagaa
     5521 tatcgaggtt gatggtttcc gcaaaggctc tgttccagag actatggtgc gcaagcacat
     5581 ccctgatcag ctcctcctag aagaaatggc agagcgtgca ctccaagaac actacgtgca
     5641 gatgcttact caagagagta ttgatgcgat cggtcgcccg cgcattgcgc tcaccaaact
     5701 cgcccgtgat aacgatttgg aatttaaaat cattaccgca gtaatgcctg aagtgaaact
     5761 ccctgactac cacgcgcttg caaaagcagc agaaaaaatt gaagcgcctg cagtaaccga
     5821 tgaagatgtt gaaaacacta ttaacgagct ccgccacatg cgcctgcacc agaacgatac
     5881 tgctggcacc cacgaccaca gcactcctca tgagcatact gaagcagagc tccccgttgt
     5941 tgatgacgcc tttgcaaaat catttggtaa atttgaaacg gttgccgatt taaaagcaaa
     6001 aattcgcgag aacttggcgc tcgaaaaaga tcgcgaagca aaagacaagc agcgcaccgc
     6061 ggtacttgaa gcagtaagca aagaaaccga ggtagtagta ccaaaacttt ttgttgaagc
     6121 agagctccaa aaattagtat cacaagtaga gtttgaagtg agccgcgctg gcgtgaagtt
     6181 tgaagactac ctaaaacagg ttggtaaaac taaagaagat ttagaaaaag aatttttgcc
     6241 cgatgccgag cgccgcgcaa aattccaact ggtacttgat gcgatcgcca ataaagagaa
     6301 cctgaagcct gaagcagaag tggtagagca ggaaactcaa aagttaatga ccatgtaccc
     6361 gggtgccgat gaagcccgca cccgtgccta cgcccaaatg gtgcttacaa acgaaaaagt
     6421 actttcattt ttggaaacac tctaaaaatt aataagtaaa aaaaccattt ttattcacta
     6481 aaaatggttt ttgaaaaaaa taaaattcta atttccaaac aagtactttt taacccgttc
     6541 aggtaaacgg tttttccaga gtggcgtatc aattgaccag taccaatgaa tacggaaaaa
     6601 ttctttcgta tctctaattt tagcctggac tgctttgtat gccaggcctg tttttttgta
     6661 gctccatatg tggagtatgc cgagcggcac caattcgagc aggtggagaa ccttcgggat
     6721 aagtattaac tctccgacat gatcaaaccg taatcggcta ttctgtacgg catgatgcaa
     6781 aagagaaata attttccacc caataataat ccatccccac accggaatag tttcaaataa
     6841 gatttgaaac acaatagata aaggagtggt aggatatagt aggtaaaaaa taccaaaacc
     6901 agcccctgta aacaagataa cagccgaagc ggtgagagaa atctgataat acacatcacc
     6961 ccacactgtt ttatcagtta tttctgtggt gcatatatgt gagaatccga tgcaggcttt
     7021 ccacataaac cacggtaaac gaataaagtt tttgataaac aacataaaat aaattttcta
     7081 aataatacat taattttaaa atatgtcaaa atttttagga aagttttttg catttggctc
     7141 aaaagcgggt atagtgagcg tgtactgtac tgaccttctt cgactgccta ccctctttta
     7201 ccacttttaa tttgaaacaa cccttatgtt aattccaacg gttatcgaaa aatcgcagtt
     7261 cggcgagcga gcgtatgata tctactctcg cctcctgcgc gatcgactta tcttcttaaa
     7321 cgaccaaata gacgaccaca ctgcaaactt ggttattgcc caactgctct ttttggcaaa
     7381 tgaagatcca aaaaaaccaa tccattttta tatcaactca ccaggggggt cggttacttc
     7441 tacgatggcg atttatgaca ccatgcagtt tgtaaaacct gaagtcttta cctactgtgt
     7501 cgggctcgct gcatcaggtg gcgcaatgct cttgctcgcc ggacaaaaag ggaagcgttt
     7561 tgcgctcccc cactcgcgca ttatgatcca ccagccactg ggcggcgctg aggggcaagt
     7621 aagcgatatt gaaattgttg cgcgagaata cagtaaaaca aaaaagacga tgaacgagct
     7681 cattgcaaag cacaccggac aaaaacttga aaaggtagaa aaagacaccg accgcgacta
     7741 ctacatggac gcgcaagagg caaaaaatac ggcatcgttg atgaagtgat taccaaagca
     7801 ccaaccatag agcgtgctga aaaactagac taagacgagc accatgaaca ccttacctat
     7861 cctgtttggc acactgcttg caggggcact gttgggctac tggctccgca tgctcattgc
     7921 actgagccgg aagggttcga ttgagcttga gaccaagcac cggctcgtag agacaaaaga
     7981 agcagcgcag cgcatcatcg acactgcaaa caaaaaagct gaagagcggc tcgcagaact
     8041 gcgcactgaa gagcacaccc gtgaagaaaa gcttcagaac actgaaaccc gactcataaa
     8101 aaaagaagaa ctcctcgaca cgcgggcagc tgaacttgat cgagaacatg aaacactcaa
     8161 agagcgcgag ggtacggtat tggcacgaga gagcgcagta caaaatgatg aagcgctttt
     8221 aacggagcgg ttagaagtta tcggcaagct caccatgagc gaagcccgag cagcactgat
     8281 tgcccgcacc gaagcggagt ttgcagaaga cctcgaactg catcggcaca aacttgaaac
     8341 tcagatgcaa gaagacattc agctgcgcgc tcgagaaata ctggcaactg caataaacag
     8401 gcttgctgtt tctgttgcca gcgaactcac cactgccacg atacagatcc cttcagaaga
     8461 tattaaagga aaaatcatcg gcaaagaggg gcgtaatatt cgcgcctttg aacgattagc
     8521 tggcgtggag ctcattgttg acgatgcacc gggtgcaatt attgtttcga gtttcgaccc
     8581 agtccgccga gcagttgctg cagaggcgct tggggaactc atcaaagatg ggcgtatcca
     8641 gcccgcaaaa attgaagagg taattcagaa ggcgcaagag aatagtaacg aaacgatgaa
     8701 gaagcgcggc accgaagcag tgtacgagtg tggcatttac aacttcgatc cacgacttgt
     8761 ttccgtcatc ggtcggctcc atttccgcac cagctatggg cagaatgtac tgcagcactc
     8821 aatcgagtgt gcacacttag ccggcatgct tgccgaagaa ttgaaggctg atgtacaggt
     8881 tgccaaagcc ggcgccctgg tgcacgacat tggcaaggca ctcgatcacg aagtacaagg
     8941 gtcgcacgtc gacatcggca tccgtattct acgccgattc aataccgacg agcggattat
     9001 cgctgccatg aaaagccatc acgacgactg tccgcatgaa acagtagaag cagtaattgt
     9061 ccaggttgcc gacatgatat cgggttcgcg ccccggcgct cgccgagata ctgctgaaaa
     9121 ttacattaag cgcctccaag aacttgaagg gctcgctgtt cgctttgccg gcgttgagcg
     9181 tgcattcgca ctccaggccg gccgcgagat ccgcattttt gtacaccctg aaaacgtaac
     9241 tgatagcgaa gcccgtacca tggcgcgcgc catcgccctt gaaatcgaat ccgaactccg
     9301 ctaccccggc gagattaaag taaccattat tcgtgaaact cgaattattg attttgcccg
     9361 ataagcgata caataggctt atatgcacga acaaccccca aaaccagatt catcagaaga
     9421 aaagaactca gctcctaaag agggcgggct tctcaaaaaa attgggcttg ctgccggaat
     9481 gatggccgcg ggcactgccg cacttggaag cgaaggtaaa acagcagaaa aagaactcat
     9541 taatcctgac cacaacattg aagccgcagc aaaaactgac accatggacc ataaggaaaa
     9601 caccgcctct tttgaatcag ggcgcagggg tggtttagaa ataacagaag gacaagagtt
     9661 gttcggatta aaagttgaag gaaaacctgt tcctgttagc agccttgata ctactaagca
     9721 atttttcttc attaagtttt ctgctgtcaa taagaaagat aacgctaatg ttggaaaaat
     9781 acttcaagca atggactctt tacatataca accgggaaac agtgaacatc ttaaaagtgt
     9841 atttttatca aaccctaagg tatcaaaaca gataggaggg tacggtatgg caattggaga
     9901 taaagtaagt gatggaaata ctggccgagc aactattaat ccagttggaa tgaagatgat
     9961 ggaagacaga gcgtctacag agctcgatat tc
//
LOCUS       DATIBQ010000041         2103 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U16294
            k141_183712, whole genome shotgun sequence.
ACCESSION   DATIBQ010000041 DATIBQ010000000
VERSION     DATIBQ010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935789
            Sequence Read Archive: SRR25158331
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:58:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 637
            CDSs (total)                      :: 603
            Genes (coding)                    :: 601
            CDSs (with protein)               :: 601
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2103
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_183712"
                     /isolate="SMAG_U16294"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1686)
                     /locus_tag="VLB02_01220"
     CDS             complement(<1..1686)
                     /locus_tag="VLB02_01220"
                     /inference="COORDINATES: protein motif:HMM:TIGR00358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - RNA exonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VacB/RNase II family 3'-5' exoribonuclease"
                     /protein_id="HSE56686.1"
                     /translation="MSDIRSEQTFEGPISISTKGVGYLKLKDINETIEIPREGLNRAF
                     HGDTVAVTVTATPQGEYPKGTVTEVKKRAKFGYAATLVAAEGRSFAVSTDARMYVRIE
                     IPEPSLHDAEIGDKVFVGISQWENPLTDPTGEVIRVLGKPLENTAEMHAIALEKGFDN
                     IYPPEVEKESAAIAARGIPLEDFADRRDFRTVTTFTIDPADAKDFDDAISVQFLENEL
                     IEVGIHIADVSHYVRTGTALDTEAAERATSVYLVDRTIPMLPEVLSNDLCSLKPDVDR
                     LTFSAIFTFDAAFNIKDSWFGRTIIHSAKRFTYEDAQEVLNSQTGPHLKELNFCLAVS
                     KYLEAKRFEAGAISIDTEEVKFKLDENSVPVGVYKKVRGDTHHLIEELMLLANRKVAE
                     FIATHDKHGNEIFVYRVHDLPAQDRMLELRDFLKRLGHTLPLKDGIIPSFELNKLLKS
                     LAGKPEEDTVQTAIVRSMAKAIYSTKNIGHYGLAFQYYTHFTSPIRRYPDLVVHRLLF
                     EYLSKRTVDKKLWEYYEHISAHASEREKQASDAERASIKYKQVEYMSYRIGKTF"
     gene            1769..2074
                     /gene="rpsP"
                     /locus_tag="VLB02_01225"
     CDS             1769..2074
                     /gene="rpsP"
                     /locus_tag="VLB02_01225"
                     /inference="COORDINATES: protein motif:HMM:TIGR00002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HSE56687.1"
                     /translation="MLTIRLQRIGRKNEAAFRVVVTDSKHAAKSGKFHEIVGSYTLKS
                     DEVKLDKERITHWMNHGAQVSDTVHNMLVKQGVIKEKKKNVLSKKAPTVKRKELKKK"
BASE COUNT          537 a          476 c          514 g          576 t
ORIGIN      
        1 gaatgtttta ccaatccggt agctcatgta ctcaacctgc ttgtacttaa ttgatgcacg
       61 ttcggcatca gatgcctgct tttcgcgctc tgatgcgtgg gctgaaatat gctcgtagta
      121 ttcccatagt tttttatcaa ccgtacgttt cgagaggtac tcaaacaaaa gtcggtgcac
      181 tactagatcg gggtaacggc ggatgggcga ggtaaagtga gtgtagtact gaaaggcgag
      241 tccgtagtgt ccaatatttt ttgttgagta aattgccttt gccatcgagc ggacaattgc
      301 ggtttgcacg gtatcttctt caggctttcc agcgagtgat tttaataatt tattgagctc
      361 aaatgaagga atgatgccgt ctttgagcgg cagcgtgtgc ccgaggcgct ttaaaaaatc
      421 acggagctcg agcatccggt cttgggctgg caggtcgtgc acccggtaca caaaaatttc
      481 gttcccatgt ttgtcatgag ttgcaataaa ttcagcaact tttctgtttg ccaagagcat
      541 cagctcttca attaagtggt gggtatcgcc acgtactttt ttatacacgc ccactggcac
      601 actgttttcg tcgagcttga attttacttc ttcagtatcg attgaaatag cccctgcttc
      661 aaagcgtttt gcttctaagt attttgaaac ggcaaggcag aagttcagct cttttagatg
      721 tggtcccgtt tggctgttca gtacctcttg tgcatcttca taggtaaatc gctttgccga
      781 atgaatgatt gttctgccaa accatgaatc tttaatgtta aatgcagcat caaaggtaaa
      841 tattgcgcta aaggtgaggc ggtcaacatc gggcttcaaa ctgcaaaggt cgttcgagag
      901 cacttcgggg agcatcggga tagtgcggtc aacgaggtac acactcgttg cccgttctgc
      961 agcttcagta tcgagggcgg ttccggtgcg gacgtagtgc gatacatcgg caatatggat
     1021 acccacttca atcaactcgt tctctaaaaa ctgtaccgaa atcgcatcat caaaatcttt
     1081 tgcgtctgcg gggtcgatgg taaaggtggt aacggtacga aagtcgcggc ggtctgcaaa
     1141 atcttcaagg ggaatgccgc gagcagcaat agcagctgac tctttttcaa cttcgggtgg
     1201 gtagatgtta tcgaagcctt tttcgagtgc aattgcatgc atttctgccg tgttttcaag
     1261 tggtttacca agcacgcgaa taacttcgcc tgttggatcc gtaagcggat tttcccactg
     1321 gctgattcca acgaatacct tatcgcctat ctctgcatcg tggaggcttg gttctggaat
     1381 ttcaattcgc acatacatcc gcgcatcggt tgaaaccgca aacgagcgcc cctctgcagc
     1441 aacgagcgtt gccgcgtagc caaattttgc tcgcttcttt acttcagtaa ccgtaccttt
     1501 tggatactca ccctgcgggg tagcagttac ggtaactgct accgtgtcgc catggaaggc
     1561 acgattaagc ccctcgcggg gaatttcgat tgtttcgttt atatctttga gtttcaggta
     1621 cccgacccct ttggttgaaa tggaaatcgg tccttcaaac gtctgctcgg agcgtatgtc
     1681 tgacatgtat ctactctaac acggcttact atataaggcg aaaaaaactg cgttgacgct
     1741 ttttttatta cctggtagta tcaagcccat gttaactatt agattgcagc ggattggccg
     1801 aaagaacgag gcggcatttc gcgtggttgt taccgactca aagcatgcag ccaagagcgg
     1861 caagttccac gaaattgtgg gctcgtacac cctcaagagt gatgaggtaa aactcgacaa
     1921 ggagcgcatc acccactgga tgaatcacgg tgcgcaggtt tcagacaccg tgcacaacat
     1981 gctcgtaaag caaggtgtga ttaaagaaaa gaagaaaaac gtgctctcaa aaaaagcgcc
     2041 gacggtaaaa cgaaaagagc tgaagaagaa atagcacttg aaaaaacacc tgaagttaca
     2101 aac
//
LOCUS       DATIBQ010000042         4000 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U16294
            k141_2018830, whole genome shotgun sequence.
ACCESSION   DATIBQ010000042 DATIBQ010000000
VERSION     DATIBQ010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935789
            Sequence Read Archive: SRR25158331
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 4000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:58:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 637
            CDSs (total)                      :: 603
            Genes (coding)                    :: 601
            CDSs (with protein)               :: 601
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4000
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2018830"
                     /isolate="SMAG_U16294"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..878
                     /locus_tag="VLB02_01230"
     CDS             <1..878
                     /locus_tag="VLB02_01230"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HSE56688.1"
                     /translation="KDLADSIRQYGLLQPVTVSRVERETPEGGLLTEYELIAGERRLR
                     ASKLAGLSQIPAIIRVGDDAMAKLELAIIENLQREDLNPVERARAFLRFVDEFKFTHT
                     QIGQKMGKSREYVSNSLRLLSLPQEILDALSAGKISEGHTRPLMMLSSRPEEQGTLFK
                     EILFRKITVREAERIARKIAVEKVRKEKYMPDPEFDRLEKTLADTLGTRVHIERKELG
                     GKITIDFFSQDDLKNLLTVFEGKEVQAAPEAPSEVAAEAEALGEPELLDDRSKEEAQK
                     SEEELDLYNIKNFSL"
     gene            complement(944..2392)
                     /locus_tag="VLB02_01235"
     CDS             complement(944..2392)
                     /locus_tag="VLB02_01235"
                     /inference="COORDINATES: protein motif:HMM:TIGR00691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HSE56689.1"
                     /translation="MNPVESILTLMEQPVSAEDKARIERAYHFAADAHRDQKRKSGEP
                     YVTHVVAAAKNCALLGMDIETIVGALLHDVLEDTAIAPQEIKTAFGENVLFLVEGVTK
                     LGRVKYRGEVRHVESMRKFFIAMAEDIRVLIIKLADRLHNVETLEHVPEEKRKRIALE
                     TIEVHASLAGRLGMGKLKGILEDHAFKYAYPKEYERTTAIFKERAPLAKQAVNNAHTA
                     VEKTLTEYKIEAAVESRIKHTYSLYRKLVKYEWNVEKVYDIIALRVLVASVADCYQVL
                     GLIHMLWKPIPGRIKDYIALPKHNSYQSLHTTVITESGIIEIQIRTHEMHEVAEMGIA
                     AHFMYKEKTTTTAPLDWLDEIKELHGIIKNPSTFLKHLHLDLFRNRIFVFTPKGDVID
                     LPEGAVPIDFAYLIHSALGEKTSAAKINGKLVALHTKLKNGDVVEIITGKNAKPSGKW
                     LDFAKTTAARKKIRAYIAEHGSFIDRFFSRNQ"
     gene            complement(2658..>4000)
                     /locus_tag="VLB02_01240"
     CDS             complement(2658..>4000)
                     /locus_tag="VLB02_01240"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HSE56690.1"
                     /translation="TTLAKTAQEAIAGVIETLHGKELVEIRSYQTKSKNAQEAHEAIR
                     PTHFEVKKSGTTDDQKKLYQLIWERAVASQMVDARIARTKISATMGTKDFPEFSATGS
                     RVIFPGWLLVDTEARGEDVELPKVTVGESLTLLSLDDLEKQTQPPSRYTEAGLVKELE
                     ARGIGRPSTYASIMSTLEERGYVTKEGRTLFPTDTGDVVSTFVEKNFETYISDDFTAE
                     MEQELDEIAAGEREYVKTLKEFYTPFSKDIKEKDKLDKLTTIEPADPKFKCPKCGSGM
                     VVKLGRGGKFLSCDRYPDCDGALTITGEELPKDKVIGIDPETGLEIKAMTGRFGPYVQ
                     LGEKPAKGTKKKKGEKAVKPKMASIPKEVDPATVTLEMALKYLSIPRTLGTHPVTGKD
                     VTANNGRFGPYVAHDGNFRSIKSPLDVYTITLDQALELLAQEKKPRGFQKKKKE"
BASE COUNT          941 a          905 c          980 g         1174 t
ORIGIN      
        1 tgaaagattt agcagattct atccgccagt acggcctctt gcagccagtt accgtatcgc
       61 gtgttgagcg agaaactccg gaaggcggct tgcttaccga gtacgaactc attgcgggtg
      121 aacgccgctt gcgcgcatca aagcttgccg ggttgagtca gattccggcg atcatccggg
      181 tgggtgatga cgcgatggca aaactcgagc tggcgattat cgagaacttg cagcgtgagg
      241 atttgaatcc ggtagagcgt gcccgcgcct tcttgcgctt tgttgatgag tttaaattta
      301 cgcataccca gattggacag aagatgggca agagccgcga gtacgtttca aactcgctcc
      361 ggctgctctc gctcccgcaa gaaattttag acgcgctctc ggcaggtaaa attagcgaag
      421 ggcacactcg tccgctgatg atgctctcga gccgaccaga agagcaggga acactcttta
      481 aagaaatctt gttccgaaaa atcaccgtcc gcgaggcaga gcgcattgcg cgcaaaattg
      541 cagtcgagaa agtgcgcaaa gaaaagtata tgcccgatcc ggaattcgat cggcttgaaa
      601 aaacccttgc tgataccctt ggcactcggg tacacattga gcgcaaagag cttggcggaa
      661 aaattaccat cgattttttc tcgcaggatg atttaaaaaa cctgcttacg gtgtttgaag
      721 gaaaagaggt gcaggctgca cctgaagcgc cgtctgaggt agctgctgaa gcagaagcac
      781 tcggagagcc agaactctta gatgatcgct caaaagaaga agcacagaag agcgaggagg
      841 aattggacct ttacaacatc aagaactttt cgctataata aacaggttcc aagtcagcat
      901 atatatcagc cgccttgagc agtacatgca ataggcggtt tttttattgg tttcgtgaga
      961 agaatcggtc gatgaagctg ccgtgctcgg caatgtaagc acggatcttt tttcgagcag
     1021 cggttgtttt tgcaaaatcg agccacttgc ccgatggttt tgcattttta ccggtaataa
     1081 tttctaccac atcaccattt tttagttttg tatgcagcgc cactagtttt ccgtttattt
     1141 ttgcggcact ggttttttcg cccagcgccg agtgaatgag gtaggcaaaa tcaattggga
     1201 ctgctccttc gggcaaatca atcacatcac ctttgggggt gaagacgaaa atgcggtttc
     1261 ggaagagatc gaggtggagg tgtttaagaa aggtgcttgg attttttatt attccatgca
     1321 actcttttat ttcatcgagc cagtcgagcg gagcggttgt tgtagttttt tctttataca
     1381 tgaagtgtgc tgcaataccc atttcagcta cttcatgcat ttcgtgcgtg cggatttgaa
     1441 tttcaataat accgctctcg gtaataacgg tagtatgcaa gctctggtag ctattatgct
     1501 ttgggagagc aatgtagtct ttaattctgc ccgggatcgg cttccagagc atgtggatga
     1561 gtccgagtac ctggtagcaa tctgcaacgc tcgccacgag tactcggagc gcgatgatgt
     1621 catagacttt ctcaacattc cattcatact tcacgagctt tcggtacaaa ctgtaggtat
     1681 gcttgatgcg cgattcaact gccgcttcaa tcttgtattc agtgagtgtt ttttcaacgg
     1741 cagtgtgtgc attgtttacg gcttgctttg caagtggtgc gcgttcttta aaaatagcag
     1801 tggtgcgttc gtattctttt gggtaggcgt acttaaatgc gtgatcttct agtatacctt
     1861 tcaactttcc catccccagg cgtccggcaa gagaagcgtg tacttcaata gtttctaaag
     1921 cgatgcgctt ccgtttttct tcggggacat gttcaagggt ttcaacattg tgcaggcgat
     1981 cggcgagctt gatgatgagc actcgaatat cctcagccat ggcgataaaa aacttgcgca
     2041 tgctctctac atgccgtact tcgccgcggt attttactcg tccgagcttg gtaacccctt
     2101 caaccagaaa gagtacattt tctccgaatg cagttttaat ttcttgagga gcaatagcgg
     2161 tgtcttcaag tacatcgtgg agcagtgcac cgacgattgt ctcaatatcc atgccaagca
     2221 gtgcacaatt ttttgctgct gccaccacgt gtgtaacata gggctcacca ctctttcgct
     2281 tttgatcacg gtgggcatca gctgcaaagt ggtacgcacg ttcaatgcgc gctttatctt
     2341 ctgccgatac cggctgttcc atgagagtga gaatactttc tacaggattc atacctatga
     2401 cgatagagca cttttaataa aaatggaaga ggaacttgcg tttttttaga ggtatgctat
     2461 agttggaccc agaatttaat gggttagtaa agtggggtta cggtagtttt caaacttgct
     2521 gtaacccttt ctttttataa aatgcacatt tttttgataa atatattgcg gtaatgggtc
     2581 gacaccgcaa gatttatagt tcacaaagtc gaccttttct catgtgacac gccctctctt
     2641 tccagagagg gctttttcta ctccttcttt ttcttttgaa agccgcgagg ctttttttct
     2701 tgagctaaaa gctcgagtgc ttggtcgagg gtaatggtgt acacatcgag tggcgactta
     2761 attgagcgaa aattgccgtc gtgtgcaacg tatggtccaa agcgcccgtt gttggcagta
     2821 acatctttac cagttactgg atgtgtaccg agcgttcggg ggatagaaag gtatttgagt
     2881 gccatctcga gcgttacggt tgcagggtca acctcttttg gaatagaagc catttttggt
     2941 tttactgctt tctctccttt tttctttttc gttcctttag ctggtttttc accaagctgt
     3001 acgtagggtc caaagcggcc ggtcattgct tttatttcaa gccccgtttc tggatcaata
     3061 ccaattacct tatctttagg cagttcttcg ccggtaatgg tgagtgcacc atcgcagtcg
     3121 gggtaacgat cacaggagag aaactttccg ccccgaccaa gttttaccac catgccgctg
     3181 ccgcactttg ggcacttaaa ttttgggtca gctggttcaa tggtagtaag tttatcaagt
     3241 ttatcttttt ctttgatgtc tttactaaat ggggtgtaaa attccttgag tgtttttaca
     3301 tactcgcgtt cgccagcggc gatctcatcg agctcttgtt ccatttcagc ggtgaagtca
     3361 tcgctgatgt atgtttcaaa atttttttca acgaacgtcg aaacaacatc gcctgtgtcg
     3421 gtagggaaga gtgtgcgccc ttctttagta acgtagccgc gctcctccag ggtcgacata
     3481 atacttgcgt aggttgaagg gcgcccaatg ccgcgcgcct cgagctcttt caccaggcct
     3541 gcctcggtat agcgtgaagg tggctgggtc tgcttctcaa ggtcgtcgag cgagagcagt
     3601 gtaagcgatt caccaaccgt aacctttggc agctctacat cttcaccgcg cgcttcagta
     3661 tctaccaaga gccagccagg aaaaataact cgcgaaccgg ttgcagaaaa ttcagggaag
     3721 tcttttgtcc ccatagtggc agaaattttt gtccgggcaa tccgcgcatc taccatctga
     3781 cttgcaaccg cgcgctccca aataagctgg taaagttttt tctgatcatc agtcgtgcca
     3841 gattttttca cttcaaagtg agtcgggcga attgcctcgt gcgcctcttg agcgtttttt
     3901 gattttgtct ggtaacttct aatttctacc agctcttttc catggagtgt ttcaataacg
     3961 ccggcaattg cctcttgagc agtttttgca agagtggttg
//
LOCUS       DATIBQ010000043         4341 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U16294
            k141_1198400, whole genome shotgun sequence.
ACCESSION   DATIBQ010000043 DATIBQ010000000
VERSION     DATIBQ010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935789
            Sequence Read Archive: SRR25158331
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 4341)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4341)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:58:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 637
            CDSs (total)                      :: 603
            Genes (coding)                    :: 601
            CDSs (with protein)               :: 601
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4341
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1198400"
                     /isolate="SMAG_U16294"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..295)
                     /locus_tag="VLB02_01245"
     CDS             complement(<1..295)
                     /locus_tag="VLB02_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HSE56691.1"
                     /translation="MHRIMLKRRITALLVLIIAAGIAYFVYGSQIKTEGTLSKYPFRL
                     GLDLSGGTHLVYKADTSQLPEGQIKDSMDALRDVIERRVNLFGVSEPVVQVQHG"
     gene            complement(326..883)
                     /locus_tag="VLB02_01250"
     CDS             complement(326..883)
                     /locus_tag="VLB02_01250"
                     /inference="COORDINATES: protein motif:HMM:NF025736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HSE56692.1"
                     /translation="MHDFNVALFRIINSLAATNHFWDTFFIVITTTALGVTLFILLGA
                     YLVWHRHDEEPSLWKRFMRKNGELFLVAVSLFFTYSIVQILKISFAIPRPFLALTDIN
                     ALLYYGANNSFPSSHAAMFTALATSVYFSHRRLGFWLFVLALVIGFSRIYVGVHYPFD
                     VLAGFCIGFLVPYTIRRIIRWGTER"
     gene            complement(876..2000)
                     /gene="rodA"
                     /locus_tag="VLB02_01255"
     CDS             complement(876..2000)
                     /gene="rodA"
                     /locus_tag="VLB02_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HSE56693.1"
                     /translation="MIRRFFAGFDTTLFAAVLPIIAAGLVTMTSFSGETSFFSRQLAW
                     ALASIVLCMGIARIDVRFLRQSRILVILYLLSLTLLAAVLVFGKTIKGSQSWIDFGGI
                     SIQPTDFAKIFVIGMLAKYFSRRHVEIANPRHLYVSFLYVLVPTGLVLLQPDFGSAII
                     ILGLWLGMALIAGISRKHLLLIGGIFLAAILVGWLFLFKPYQKARIISFVSPMSDIRG
                     SGYNAYQSMIAVGSGQFLGKGVGYGTQSRLNFLPEYQTDFIFAAFAEEWGFVGALIIL
                     ACFGVIIWRLITHASRGASNFETLFCAGFAIMLLGHLVINVGMNIGLMPITGLPLPFM
                     SYGGSHLLAEMIGLGIVFSMSRYERAIHRDDLQNEFVGYA"
     gene            complement(1997..2779)
                     /locus_tag="VLB02_01260"
     CDS             complement(1997..2779)
                     /locus_tag="VLB02_01260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56694.1"
                     /translation="MNWAVRRRFLYVLITFLAAGTLFAIVFRNVLYQAPTCFDSKQNG
                     EEVGIDCGGGCARYCPNTLALPKVRWKRSFPIASGFAHAVASIEHNYPNTGLRSLTYE
                     FTLLDANNERITTRRGTTFIGGHGRSAIIEPLIPVGKQVPVVTQFEILSSDSWEKFPA
                     GVDDSTFKVERTDLETLVDATRLTATLTNQSASVLRNVQVIAILYDTKDNAITASKTE
                     LQSVASLGRVTAYFTWDFALPRDVARIEIIPRVNPFTDVIPQ"
     gene            complement(2844..3608)
                     /locus_tag="VLB02_01265"
     CDS             complement(2844..3608)
                     /locus_tag="VLB02_01265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56695.1"
                     /translation="MYINTTLIIILCLIAAVAFFFFIRSTLKKNRLVEKHEAVIKKFS
                     FNTEYSLCEHVEEIISLAWKWQTKTIYCLLSGYGECIMRGLRNKLMAMGSLSPKIILE
                     YLEKHGYTYKQEWRLNTKDQAGTELLRKIWRAAILRYLGDCQNRFEYAFRFMYSLTEA
                     PSVSDLKVWHEDFLVCQDIQRSLSSLYFIEKMIEEMESYFFQRVVSKIEMDPSITVNE
                     LNEIESILKSVVPVNSNQLNNLIVLMHDYKKKVGIK"
     gene            complement(3698..3805)
                     /gene="rrf"
                     /locus_tag="VLB02_01270"
     rRNA            complement(3698..3805)
                     /gene="rrf"
                     /locus_tag="VLB02_01270"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     misc_feature    complement(3888..4127)
                     /note="23S ribosomal RNA rRNA prediction is too short"
BASE COUNT         1310 a         1033 c          918 g         1080 t
ORIGIN      
        1 tgccgtgctg cacctgtacc accggctctg aaacaccaaa caaatttacc cggcgctcaa
       61 tcacatcgcg gagcgcatcc atagaatctt taatctgacc ttcagggagc tgtgaggtgt
      121 cagccttgta caccaggtgc gttccgcctg aaagatctaa tccgagccgg aaaggatact
      181 ttgagagtgt cccctcggtt tttatttgtg aaccatacac aaaataggca ataccggctg
      241 caataatcag tactagtaaa gcagtaattc ttcgtttcag cataatacgg tgcattctat
      301 catggttacc ttttctcgca atatattagc gctcggtacc ccatcgtata atacgccgga
      361 tcgtatacgg cactaagaag ccaatacaga acccagcgag gacatcaaac ggatagtgca
      421 ctcccacata aatacgagaa aacccgataa cgagtgccag cacaaagagc cagaagccaa
      481 gccgacggtg ggagaagtac accgaggtag cgagtgcggt aaacatcgcc gcatgacttg
      541 aagggaatga attgtttgca ccgtaataga gcagtgcgtt aatgtcggtg agtgctaaaa
      601 atggtcgtgg gatcgcaaat gaaattttta aaatctggac aatgctgtag gtaaaaaata
      661 gcgagacagc aacaagaaag agttcgccat tctttcgcat gaagcgcttc caaagtgaag
      721 gttcttcatc atgtcgatgc cacactagat acgcccctaa aagaataaac agtgttacac
      781 cgagagcggt tgttgtaata acaataaaaa aagtatccca aaaatggttg gtagcagcca
      841 agctattaat tattcgaaat agtgcaacgt tgaaatcatg catagccgac aaattcgttc
      901 tgcagatcat cgcggtggat cgcccgttcg tagcgagaca ttgaaaatac aattcccaga
      961 ccgatcattt ctgctaagag gtgcgacccg ccgtaactca taaacgggag cggcaacccg
     1021 gtaatgggca tgagcccgat attcattccc acattaatta ctaagtgccc taagagcata
     1081 atagcaaagc cggcacaaaa gagcgtttca aaatttgagg caccccgcga ggcatgagta
     1141 atgagtcgcc agataatcac cccaaagcag gcaagaataa tgagtgcgcc cacgaacccc
     1201 cactcctcgg caaaggcagc aaagataaaa tcggtttgat actcaggcaa aaaattgagt
     1261 cgcgactgcg taccgtaccc tacccccttc cctaaaaact gccctgaacc aaccgcgatc
     1321 atcgactggt acgcattgta tccactcccg cgaatgtcgc tcatcgggct cacgaacgaa
     1381 atgatccgcg ccttttgata gggtttaaag aggaatagcc agccgaccag aatagctgcc
     1441 aaaaaaattc cccctatcaa cagcaagtgt ttacgtgaga taccagcaat gagcgccatg
     1501 ccgagccaga gcccgagaat aataattgcc gagccaaagt cgggctgcaa gagcacgagc
     1561 ccggtaggaa caagtacata caggaatgac acatagaggt gccgtggatt tgcaatctcg
     1621 acatgacggc gcgagaagta tttagcgagc atcccgatca caaaaatttt tgcaaaatcg
     1681 gttggttgaa tagagatacc accaaaatcg atccagctct gtgaaccctt tattgttttt
     1741 ccaaacacga gcaccgcagc caaaagggtg agcgagagca ggtacaaaat aaccagtatt
     1801 cgcgactgcc gaagaaaccg aacatcaatg cgagcaatgc ccatacagag aacaatagaa
     1861 gcaagcgccc acgcaagttg gcgggaaaaa aaagaggttt cgcccgaaaa cgaggtcatt
     1921 gttaccaagc cagcagcaat aatcggtaac actgccgcaa ataaggtagt atcgaaccct
     1981 gcaaaaaaac gcctaatcat tgtgggataa cgtcagtaaa cggattgacg cgaggaataa
     2041 tttctattcg agcaacatcg cgcggcagtg caaaatccca ggtaaagtac gccgttactc
     2101 gaccgagcga agcaacggat tgcagctcgg ttttactcgc tgtaattgca ttgtctttag
     2161 tatcatagag aatcgcaatc acctgtacgt tacgaagtac tgatgccgat tgattggtga
     2221 gcgttgccgt cagacgagtt gcatcgacca gtgtttcaag atcagtacgc tcaaccttaa
     2281 acgtactgtc atccacccct gcaggaaatt tctcccacga atcactcgag agaatttcga
     2341 attgcgttac caccggtacc tgcttaccca cgggaataag cggctcgata atagctgagc
     2401 gcccgtgtcc accaataaag gtagtaccgc gacgagtagt gatacgctca ttgttcgcgt
     2461 ccaagagagt aaattcgtag gtgagagagc gcaagccagt atttggatag ttatgctcga
     2521 tagaggcaac tgcgtgagca aacccgctgg caataggaaa gctgcgcttc cagcgaacct
     2581 tgggaagcgc gagcgtgttt gggcaatacc gtgcacagcc cccgccgcaa tcaatgccaa
     2641 cttcttcccc attctgttta ctatcgaaac aggttggtgc ctggtagagt acgttccgaa
     2701 acacaatagc aaagagcgtt cctgcagcaa ggaacgtaat gagtacatat aaaaaccttc
     2761 gacgcactgc ccagttcatc cccttcagta taacaaaaaa gcccgctctg cggcgggact
     2821 tctattacac aaatgtaagt acgttatttt attccaactt tcttcttata gtcatgcata
     2881 agcactatga gattatttaa ctggttacta tttactggca ccacagattt caaaattgat
     2941 tcaatttcat tcaactcgtt aacagtaatc gatggatcca tttcaatttt tgacaccact
     3001 ctttggaaga aatagctttc catctcttcg atcatttttt caataaagta aagtgaggaa
     3061 agagaacgct ggatgtcttg gcaaaccaaa aaatcttcat gccatacttt caagtctgaa
     3121 acagaaggag cctctgtcaa actatacata aacctaaaag catattcaaa tctattttga
     3181 cagtcaccca gataacgtag tatcgctgct ctccatattt tccgaagtaa ttcggttccc
     3241 gcttggtctt ttgtattcaa tcgccactcc tgcttgtatg tatagccatg cttttcaagg
     3301 tattccaaaa taattttagg agaaagactc cccattgcca taagcttatt acgaagtcct
     3361 cgcataatac attctccata accagaaagg aggcagtata tagtttttgt ctgccacttc
     3421 catgccaagg aaattatttc ctctacatgc tcacataaag aatattccgt attaaatgaa
     3481 aattttttaa taactgcttc atgtttttcc accaagcgat tcttttttag agtagaacga
     3541 ataaagaaaa aaaatgccac agcagcaatt aggcagagaa taataatgag tgttgtgttt
     3601 atatacataa caatgatttt tgttcattat acatataatt attaaaatgt caaaaaccca
     3661 ccttcatggg gtgggttttt gtgcattaaa cactcagtcc tggcgactac ctacttttgc
     3721 ccttgaggac tatcatcggt tctacagggc ttaactactc tgttcggaat gggaagaggt
     3781 gtgacccctg cgacaaatca ccaagacaga gtgtttaatg ctgctgaacg ttctttttta
     3841 tacttccgtc ttgcaacggg tatgatttcc agatttccta ataggaatcg ggcaattagt
     3901 acttctcggc tgaacgcatt gctgcgcgta cacctaaagc ctatcaacct agtcatctcc
     3961 tagggccctc aaacgatacc taatcttgga gctggcttcc cacttagatg ctttcagtgg
     4021 ttatccattc cgaacatagc tactgggcag tccacctggc ggtagagccc atagaccaga
     4081 ggttcgttca tttcggtcct ctcgtcacgt atattcccct atttctagga gtgtagacta
     4141 taccttcttc cgaatagtat cggaaggctg catcttacct gtacgagcag tcattctcgg
     4201 atggaatgat tcctctacgc acaagtcttg ctccgaagag caaagtcgtt acggggtcaa
     4261 atcaaacaat tgcagcaact cttctctagt tttctttttc ttggtgacat agttttcact
     4321 ttgtatcaca agaataaaat c
//
LOCUS       DATIBQ010000044         7158 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U16294
            k141_1419279, whole genome shotgun sequence.
ACCESSION   DATIBQ010000044 DATIBQ010000000
VERSION     DATIBQ010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935789
            Sequence Read Archive: SRR25158331
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 7158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:58:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 637
            CDSs (total)                      :: 603
            Genes (coding)                    :: 601
            CDSs (with protein)               :: 601
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7158
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1419279"
                     /isolate="SMAG_U16294"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..972)
                     /gene="ligA"
                     /locus_tag="VLB02_01275"
     CDS             complement(<1..972)
                     /gene="ligA"
                     /locus_tag="VLB02_01275"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HSE56696.1"
                     /translation="MTEALARERILKLKAEIERLRFAYHVHNAPGVTDDVYESLMREL
                     HALEEKFPQFAVSESLNRIAGKPLDKFRKVTHSSRMLSLQDAFGAEELYAWEKRVKKL
                     LEGKPYRYFAELKLDGLAVNLRYEQGVFVQGATRGDGFVGEDITENIKVIGDIPLRLL
                     PPYPQHIEVRGEVVMQKSVLERLNKEQEEKGLPKFANTRNAAAGSVRQLDPSIAQSRQ
                     LSFFAYDIAVLQGAQKIPEFHSEEHALLRELGFPVTSQECVAKTLPEIETFIAAIGEE
                     REHLPYHIDGIVVQVDELSLHDRLGVVGKAPRYAVAFKYPAERATTMV"
     gene            complement(976..1464)
                     /locus_tag="VLB02_01280"
     CDS             complement(976..1464)
                     /locus_tag="VLB02_01280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56697.1"
                     /translation="MNKKNSKKKYPTQAGFAMLFTVLLISLVLSLALGISNITYKQTI
                     LSSLAKDSQIAFYQADAGAECGLYYDFTLNAFPEPSTFAAIQSTVPTITCGTETLTLD
                     PADSSDDDFVYRSSPASPSNPCYNVVFDKTGVENIIQAYGYNICDQDHPRQVERALQV
                     RY"
     gene            complement(1469..2062)
                     /locus_tag="VLB02_01285"
     CDS             complement(1469..2062)
                     /locus_tag="VLB02_01285"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HSE56698.1"
                     /translation="MKKPQPLQSGFTLVETLVAILMLTLTMGALFTLASGGLFSVRYA
                     RNQLVANNLLQETIESVRNDRDTAVQNNVPWDAWLGTYNVDTNGSMQDPGDLSSGCFS
                     SNGCTVDSYAGTVKACSTDSCQHLTFYPNSNFYGYESSYPGVQTNGAYSTSYARKIQF
                     VSTSNPDQYIVTGEIKWNNGTATKTLTQSMLLTKWQP"
     gene            complement(2065..2607)
                     /locus_tag="VLB02_01290"
     CDS             complement(2065..2607)
                     /locus_tag="VLB02_01290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56699.1"
                     /translation="MVSLSLFTVVMLATISSLLIVNDASRKVQSMRAVMDNLNFAVEA
                     MSRTIRTGSEYICDAGNTGPNVSCPFADGNPADSIRVRSEESTPGNDIYVDFRLGSAG
                     GVGVLERQRTVGGVPTGWQAMTSSEIDVQELQFYVDGAETSDLTQPSVTLFIKGTAAT
                     NSTQVAPFSIQTKISQRTIE"
     gene            complement(2677..3336)
                     /locus_tag="VLB02_01295"
     CDS             complement(2677..3336)
                     /locus_tag="VLB02_01295"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HSE56700.1"
                     /translation="MRKKQQYNAGLTLIEMLVVLGIFATVSGILLFNYGDFRNNVSIR
                     NLSQEVALAVRKAQTYATSVRGTSIGGSTRDYPAYGIAFASTAAVPDLAPDQKEIVLF
                     VDVPPGADPELNGDTYYSYGSATCGAVSEGEECFEKLQIATTDKIKKVEVCTSSFTCS
                     EVPEVYISFQRPNPDAVFCVPSGAVCDSTVYSSAILTLESAVGLQKKVSVWNTGQISV
                     Q"
     gene            complement(3333..4118)
                     /locus_tag="VLB02_01300"
     CDS             complement(3333..4118)
                     /locus_tag="VLB02_01300"
                     /inference="COORDINATES: protein motif:HMM:NF013632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HSE56701.1"
                     /translation="MLFIPVLVFIFGTIIGSFLNVVIYRINTGRSVAKGRSKCAICST
                     TLHWQELVPVFSFLALGGKCKTCRTKISFQYPVVELLSGLLFTVLYIHILIPAWFSPL
                     AWLEFCFVAALVSLALVIGAYDFRHKIIPVVPMWITLALSIVALGARLFLDHSFPIVE
                     ALVAGPLVAAPFFLLWGISRGRWMGFGDVKLALALGWLFGIGSGFTVVLVSFWIGGLT
                     GLFLLALSNRYSMQSQVPFAPFLLVAALVVYFWSLSLSSFLPL"
     gene            complement(4191..4682)
                     /locus_tag="VLB02_01305"
     CDS             complement(4191..4682)
                     /locus_tag="VLB02_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014686290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HSE56702.1"
                     /translation="MKKNFNKGFTLIELLVVIAIIGILASVVLASLNSARTKGADAAV
                     KSNLANARAQAELFYDANSNSYDGGTNATDVCDPAGVVSTIKGISANVVAAATAGGAT
                     AGVVVNNDTSTPTEASCNSSASAWAAESPLKTAGQYYCVDSTGTAKQGGAAMLTGVAD
                     YTC"
     gene            complement(4791..5996)
                     /locus_tag="VLB02_01310"
     CDS             complement(4791..5996)
                     /locus_tag="VLB02_01310"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HSE56703.1"
                     /translation="MNYRYTAVTNTGEKREGYIEATSRELAVSGLQRRDLIVTSIKSE
                     SERKKWFEITLFEKIPMKEVVILSRQMSTLFEAQVSALKAFSLLATNTASKPLQRKLE
                     TIVTDLQAGSSIANALAKHKAVFSDFYINMVRAGEESGKLTQVFEYLADYLDRQYALT
                     SKTKHALVYPAFVIGIFFIVMTIMFTVVIPKLSSIITESGQDIPIYTKIVIGISNFLV
                     NYGIFLLIFLIVLFGYLFHLSRTVRGKQYLDEIKLSVPVLGSLFRKLYLARIADNMDT
                     MLSSGIPIVRAIEITGSVVGNKRFQGILTEAVQAVKSGRSFSQSLEVHKEIPTIMPQI
                     IRVGEETGSLGNILKMLAKFYKREVDEAVDTLVGLIEPFMIITLGLGVGLLLVSVLAP
                     IYNIAGGIS"
     gene            complement(6003..7061)
                     /locus_tag="VLB02_01315"
     CDS             complement(6003..7061)
                     /locus_tag="VLB02_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HSE56704.1"
                     /translation="MDYKTELNELIQILIREAGSDLYISSARFPTIRVNGELVFLVTR
                     TQYSNDDALATLKILLSKEQYDRFIEQQEIDFSYEFQKGVRLRGNAFIQRGMCSIVMR
                     LIPKVKTLEELNLPPILAEFARKRQGFFLVVGPVGSGKSSTMSAMVNIINHERAAHIL
                     TVEDPIEYVYDTGRSIVDQREVGIDTADFSVALKSAFRQDMNVILIGEMRNPETIATA
                     VTAAETGHLVLSTLHTNTASQTIDRIIDSFPPGQQDQIRIQLASSLIGVFSQRLVPRI
                     TGGRLPAYELMMNNNAVSNLIREKRTHEIDVVIETGYEQGMIDMNRSLVDLVRRGEIS
                     VENAFAFSTNPKTLERLL"
BASE COUNT         1943 a         1748 c         1610 g         1857 t
ORIGIN      
        1 taccatggtg gtcgctcgct cggcaggata cttaaacgct accgcatagc gtggcgcctt
       61 ccccaccacc ccaagccgat cgtggagtga aagttcatca acttgcacca cgatgccgtc
      121 aatgtgatag ggcaagtgtt cgcgctcttc gccaatagca gcaataaagg tttcgatttc
      181 cggaagtgtt tttgcaacac actcttggct tgtaaccgga aatccaagct cacgcaaaag
      241 tgcatgctct tctgaatgaa actcaggaat tttttgagca ccctgcagca cggcaatatc
      301 gtatgcaaaa aaagaaagtt gtcgagattg agcaatgctt ggatcaagct ggcgcactga
      361 acctgctgct gcgttgcgcg tgttggcaaa ttttggcaac cctttttctt cctgctcttt
      421 gtttaaccgt tcaagcaccg acttctgcat caccacttct ccgcgcactt caatatgctg
      481 tggatagggc ggcaacagcc gcagtggaat atcaccaatt acttttatgt tctcggtaat
      541 gtcttcaccg acaaatccgt ctccacgggt ggcaccttgc acaaatactc cctgctcata
      601 acggagattc accgcgagcc catcaagctt tagttcagca aagtatcggt acggttttcc
      661 ttcaagtagt tttttaacgc gcttttccca ggcgtacaat tcctcggcac caaacgcatc
      721 ctggagcgag agcatccggc tgctgtgtgt tacttttcta aatttatcga gcggctttcc
      781 tgcaattcga ttcagactct ctgacactgc aaattggggg aacttttcct caagtgcatg
      841 gagttcgcgc atgagtgatt catacacatc atcggttact ccgggcgcat tgtgtacatg
      901 gtacgcaaaa cgaagccgct caatctcggc tttcagtttt agtattcgct cacgggcaag
      961 cgcttcggtc ataggctagt accgtacttg cagcgcacgc tcaacttggc gcggatggtc
     1021 ttgatcgcag atgttatacc catacgcctg aatgatattc tcaacgccgg ttttgtcaaa
     1081 aacaacgttg tagcaggggt ttgagggaga tgccggactg gaacggtaca caaaatcgtc
     1141 atcactacta tcggctgggt ctagggtaag tgtttccgta ccgcaggtaa tagtaggaac
     1201 agtcgactgt atagcagcaa aggtactcgg ttcaggaaat gcgttgagcg taaaatcgta
     1261 gtacaaaccg cactcagcac cggcgtctgc ctgataaaat gcaatctgtg aatctttagc
     1321 gagtgaggag agaatagttt gtttgtaagt aatatttgaa ataccgagcg cgagcgagag
     1381 taccaaacta ataagcagta ccgtaaagag cattgcaaac cctgcctgtg ttggatattt
     1441 ttttttgctg ttttttttat tcatacattt acggctgcca cttcgttaat aacatcgatt
     1501 gcgtgagcgt ctttgtagct gtgccattat tccacttaat ttctccggtg acgatatatt
     1561 gatcggggtt actcgttgaa acaaattgta tctttcgagc gtatgaggtg gagtatgcgc
     1621 catttgtctg caccccaggg tacgatgatt cgtatccata aaaatttgag tttgggtaga
     1681 aggtaagatg ctggcaacta tcggttgaac acgcttttac cgtgcctgcg tatgaatcaa
     1741 ccgtacagcc gtttgatgag aaacagccag aagagagatc gcccggatcc tgcatacttc
     1801 cattagtatc aacgttgtac gtgccaagcc aagcatccca cggaacatta ttttgcactg
     1861 cggtatcgcg gtcgttacgc actgattcaa ttgtttcttg caacaaatta tttgccacca
     1921 gttgattgcg agcataacgt actgaaaaga gcccgccgga agccaaggta aagagtgccc
     1981 ccatggtgag tgttaacata agaatagcga caagtgtttc taccaatgtg aaccctgatt
     2041 gtagtggttg tggttttttc atacttactc aatcgtgcgc tgactaattt ttgtttgaat
     2101 cgagaacggc gccacctggg tactattggt agctgccgtc cccttaataa agagcgttac
     2161 tgaaggctgc gtaagatcgc tcgtctctgc cccatcaaca taaaactgca actcttgaac
     2221 atctatctct gatgaagtca ttgcctgcca gccggttggc acaccgccaa cagtacgctg
     2281 gcgctcaagt actcctaccc cgcccgcaga accgagacgg aagtcgacgt aaatatcatt
     2341 gcccggggta ctctcctctg aacgaacccg aatactatct gcagggttgc catcagcaaa
     2401 cggacaacta acatttggtc cggtatttcc tgcatcgcat atatactctg agccggtacg
     2461 aatcgtgcgc gacatcgcct caacagcaaa attcaagtta tccatcactg ctcgcatcga
     2521 ttgcaccttg cgagaagcat cgttgacaat aaggagtgag cttatcgttg caagcataac
     2581 gaccgtaaac aaactcagtg ataccataag ctcaactaag gtaaatccct cgtttctctg
     2641 tttttttagt tgtctagtgt gggtacgtcg catatattat tgaacactta tttgtccggt
     2701 attccagacc gatacttttt tttgcagacc gacagcggac tcaagcgtaa gtatagctga
     2761 tgaataaaca gtactatcac atactgctcc actaggcaca cagaacaccg catcggggtt
     2821 tggccgctgg aagctaatgt agacttcagg aacttctgaa caggtaaagc tgctagtgca
     2881 cacttcaact ttttttattt tatcggtggt agcaatctgg agcttctcaa aacattcctc
     2941 cccctctgaa acagcaccgc acgttgcgct cccgtacgag tagtacgtat caccattgag
     3001 ttccggatca gcacccgggg gtacatcaac aaaaagtaca atttcttttt gatcgggagc
     3061 aaggtcagga acggccgcgg tggatgcaaa cgcaattccg tatgccggat aatcgcgggt
     3121 actgccgcca atactggtac cgcgaacgct cgttgcgtat gtttgcgctt tacgcactgc
     3181 aagcgcgacc tcttggctta agttacgaat tgaaacgttg ttccgaaagt ccccgtagtt
     3241 aaataagagt atccccgaca ccgtagcaaa aataccaagc acgacaagca tttcgataag
     3301 agtgagccct gcgttgtact gctgtttttt tctcatagcg gtaaaaatga gcttagcgag
     3361 agcgaccaaa aatatacaac gagcgcggca acaagcaaaa acggggcgaa gggtacctgc
     3421 gactgcatgc tgtagcggtt tgagagcgcc aaaaggaaaa gccctgttaa accgccaatc
     3481 caaaacgaaa ccagtaccac tgtaaagccg ctgccaatac caaacagcca tcccaacgca
     3541 agcgcaagtt ttacgtcgcc aaaccccatc catcggcccc gagaaatgcc ccagagcagg
     3601 aaaaacggtg ctgcaacaag tggtcctgcc accaacgcct caactatcgg gaatgagtga
     3661 tcaagaaaga gccgagcacc aagcgcaaca atactcagtg ccagcgtaat ccacattggt
     3721 actacaggga taattttatg ccgaaaatca tatgcaccaa taacaagtgc gagtgatacc
     3781 agtgctgcaa caaagcaaaa ctcgagccag gcgagcggcg agaaccacgc cggaatgagt
     3841 atgtggatat agagcacggt aaagagaagc cctgagagga gttcaaccac tggatactgg
     3901 aacgaaattt tagtacggca cgttttacac tttccgccga gcgctaaaaa cgagaatact
     3961 ggaacaagct cttgccagtg gagcgtcgtg ctgcagatgg cacacttcga gcgccccttc
     4021 gccacactgc gaccagtgtt aattcggtaa atcaccacgt ttaaaaagct accgatgata
     4081 gtgccaaata taaatacaag caccggaata aatagcatgt actattaagt ataccgtact
     4141 aaaattaaaa acacccgcaa agtgcgggtg tttttaataa acaacttatc ttaacaagta
     4201 tagtcagcta caccggtaag catagcagca ccgccctgtt tcgctgttcc agtactgtca
     4261 acacagtagt actgaccagc agttttcagc ggtgattctg cagcccatgc agatgcacta
     4321 ctattacatg aagcttccgt aggagttgaa gtatcgttgt ttaccacaac accagcagta
     4381 gcaccgcctg ctgttgcggc agcaaccacg ttggcactaa taccttttat agtactcact
     4441 acaccagcag gatcacaaac atccgttgcg tttgttcctc catcgtagct attactattt
     4501 gcatcataaa agagctcagc ttgtgcgcga gcatttgcca agttcgattt taccgcagca
     4561 tcagctcctt tggtacgagc tgagttaaga gaggcaagca ccactgaggc caagataccg
     4621 atgatggcaa taaccaccaa tagttcaatc agggtgaagc ctttgttaaa attcttcttc
     4681 ataataaagt tatctttttt tcgaatctga taataatcga ttattacaaa ttcctttgta
     4741 atggaacgat tataccacgc accttccccc gccacgcaag ggggtgtgga ttaagatata
     4801 cccccagcaa tattgtaaat tggtgccaag acggaaacga gcagtaaacc aacacccaag
     4861 ccaagagtaa tgatcatgaa cggctcaatg agtccaacga gcgtgtcgac tgcctcatca
     4921 acctcgcgct tgtagaattt agcgagcatt tttaaaatat ttcccaaact tcccgtttct
     4981 tcaccgactc gaataatctg cggcataatg gttggaattt ctttgtgcac ttcaagtgat
     5041 tgtgaaaaag agcgtccact ttttaccgct tgcactgcct ctgtcaaaat gccttgaaaa
     5101 cgtttgttgc ccaccactga accagtgatc tcgattgcgc gcacaatcgg aatccccgat
     5161 gagagcatcg tatccatgtt atcagcaata cgagcaaggt acagctttcg gaacaggctg
     5221 cctaataccg gtactgaaag ttttatttcg tctaagtact gcttgccccg cacggttcga
     5281 ctcaggtgga ataggtagcc aaacaataca attaaaaaaa tgagtaggaa gataccgtag
     5341 tttactaaaa agttactaat gccgatcacg attttggtat aaatcggaat atcctgcccc
     5401 gattcagtaa taattgatga gagctttgga atcaccacgg taaacatgat agtcatgacg
     5461 ataaaaaaga taccgatcac aaatgcgggg tataccagcg catgctttgt ttttgaggta
     5521 agcgcgtact gacggtcaag ataatcagcg aggtattcaa acacttgcgt gagcttccct
     5581 gactcttctc cggcacgcac catgtttatg taaaaatctg aaaacactgc cttgtgcttg
     5641 gcaagtgcgt ttgcgataga agagcccgct tgcaggtcgg taacgatagt ttcaagttta
     5701 cgctggagcg gcttgctcgc cgtatttgtt gctaagagcg aaaaagcttt gagggcggaa
     5761 acttgcgcct caaacaaggt ggacatctgc cgcgagagaa tcaccacctc tttcattggg
     5821 attttttcaa atagagtaat ctcgaaccac ttctttcgct cgctctcgct ctttattgac
     5881 gtaacaatta aatcgcggcg ctgtaaaccc gacacagcaa gctcgcggct cgtagcttca
     5941 atgtagcctt cgcgtttttc tccggtattt gtaacggcag tgtatcgata attcatgtgt
     6001 tgctataaaa gacgctcaag tgtttttgga ttggttgaaa acgcgaaagc gttttcaacg
     6061 ctaatttctc cgcgacggac gaggtcgact aaggaccgat tcatgtcgat catcccctgc
     6121 tcgtagccgg tttcaatcac gacatcaatt tcatgggtgc gcttttcacg aatgagattt
     6181 gagacagcat tgttgttcat catcagctcg tacgccggca gtcgcccgcc ggtaatacgc
     6241 ggcaccagcc gctgcgagaa cacaccaatg agcgacgagg caagctggat gcgaatttga
     6301 tcctgctgcc cgggtgggaa tgaatcgata atgcgatcaa tggtctggct tgcggtgttg
     6361 gtatgcagtg ttgagagcac caagtgccca gtttcagctg cagttactgc ggttgcaatc
     6421 gtttctggat tgcgcatctc gccaatcaaa ataacattca tatcttggcg gaatgctgat
     6481 ttgagcgcta ctgagaaatc agcagtatca atccccactt cacgctggtc aacaatcgag
     6541 cggccagtat cgtacacata ctcaatagga tcttcaaccg taagaatgtg tgcggcacgt
     6601 tcgtggttaa taatatttac cattgccgac attgtagagc ttttaccact ccctacagga
     6661 cccaccacca agaaaaatcc ctggcgcttg cgagcaaact cagccaaaat gggcggcagg
     6721 ttcagctctt ccaatgtttt tacctttgga attaagcgca tcacaataga gcacataccc
     6781 cgctgtatga aggcattgcc gcgcaagcgc accccttttt gaaattcgta tgaaaaatcg
     6841 atttcctgct gctctataaa ccgatcatac tgctctttcg agagaagtat ttttaaggtc
     6901 gccagagcat cgtcgtttga gtactgtgtt ctcgttacca aaaacacgag ctcgccattt
     6961 acccgaatag taggaaagcg cgcagaagaa atgtataaat ctgatccggc ttcccgtatc
     7021 agtatttgta tgagttcatt cagttctgtt ttgtagtcca tatattatgt agttactgtt
     7081 tctttaagaa gtgcactgac ccgttcgacg acttctgacg gtgtgctgtt tgccttcaca
     7141 atgtaatttt ttgccccg
//
LOCUS       DATIBQ010000045         7614 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U16294
            k141_2186650, whole genome shotgun sequence.
ACCESSION   DATIBQ010000045 DATIBQ010000000
VERSION     DATIBQ010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935789
            Sequence Read Archive: SRR25158331
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 7614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:58:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 637
            CDSs (total)                      :: 603
            Genes (coding)                    :: 601
            CDSs (with protein)               :: 601
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7614
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2186650"
                     /isolate="SMAG_U16294"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..802)
                     /locus_tag="VLB02_01320"
     CDS             complement(<1..802)
                     /locus_tag="VLB02_01320"
                     /inference="COORDINATES: protein motif:HMM:NF013570.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
                     /protein_id="HSE56705.1"
                     /translation="MMTSAEIRKRFLDFFAKRQHAILPSASLVPENDPSVLFNTAGMQ
                     PLAPYLLGKKHPLGNRLASCQKCVRTGDIDEVGDNRHLTFFEMLGNWSLGDYFKAEAI
                     EWSYKFLTSKEEGLGLDPARLYVTVFEGDENAPRDTEAAALWKQYVPENRIYFLPAKN
                     NWWSPGDNGPCGPDTEMFYDTTTEGLGDMTHDEFMAADDRGEVVEIWNDVFMEYEKKG
                     GKVIGKLVQKNVDTGSGLERITMVVQGKSNVYEIDTFVPAIEIINANTS"
     gene            complement(844..1344)
                     /locus_tag="VLB02_01325"
     CDS             complement(844..1344)
                     /locus_tag="VLB02_01325"
                     /inference="COORDINATES: protein motif:HMM:NF014373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HSE56706.1"
                     /translation="MYTAVMGIDQVFVNLAIAMFLGMVIGLERFVAGKTAGMRTYTLV
                     SLGSALFVTISQIVAAQFSINQTFDPLRVAAQVVAAAGFLGVGAIFHRNNQVSGVTTA
                     SGLWVAAGIGLAAGFGLYEIAIMVTVLTLFIFIALWFIERQISKISARSNKNFNLSEQ
                     HPELEE"
     gene            complement(1390..2433)
                     /gene="mnmA"
                     /locus_tag="VLB02_01330"
     CDS             complement(1390..2433)
                     /gene="mnmA"
                     /locus_tag="VLB02_01330"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003232098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HSE56707.1"
                     /translation="MSKQQKKVFVGISGGVDSSVSAHLLKKAGYEVHGVFIKTWAPDW
                     VPCTWVDEKRDAMRVCAALGIPFHFLDCEAEYKTAVADHMIAEYRAGRTPNPDVLCNR
                     IIKFGSFLQYALAQGADYIATGHYAQVKKEDGYQLLKGADAAKDQSYFLWMLTQADLA
                     HTLFPIGHLQKSEVRRIAAKANLFTAEKKDSQGICFLGEVDMKDFLRHYITPRQGAVV
                     DTSGNVIGTHDGVWFVTLGERHGFTVTVRADAGPYYVVKKDIDKNTITVASHYIPESE
                     LVSEIILKNINQIIPWEAGKRYQAQLRYHGELVSCTISGEKIIFEKPVLATPAQSLVL
                     YDGNTCVGGGVII"
     gene            complement(2565..3104)
                     /locus_tag="VLB02_01335"
     CDS             complement(2565..3104)
                     /locus_tag="VLB02_01335"
                     /inference="COORDINATES: protein motif:HMM:TIGR00573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HSE56708.1"
                     /translation="MLNQTFAFIDVETTGLSTETHEIIELGAVLARVTDGALEVVDQV
                     DLKVKPEHIETADQQALRVNNYDEGAWLFAYTLEEAIKTFAKKTNGAIMIAHNLAFDY
                     GFISKAFEKTGIANDMHYHKLDTISIAFAKLAGQGDVNKFSLNALCQYYGIENKRAHS
                     AFSDAYATYEVFKKLMGLK"
     gene            complement(3112..4098)
                     /gene="mltG"
                     /locus_tag="VLB02_01340"
     CDS             complement(3112..4098)
                     /gene="mltG"
                     /locus_tag="VLB02_01340"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HSE56709.1"
                     /translation="MEYDTLPPRRRRHSLLILSILIFLLGVGGYLFGLNRPPESFPVP
                     YQFTIDPGQSLFSISGELQAEGAIQSRRLLEFWMLLFGTDRAISEGEYYFTKPATVFQ
                     MALRITGRQFGIDRKRVTFPEGFTSTDMAARLSETFTNFDGALFLNLAKEHEGYLFPD
                     TYAFFPSVTPDVVVTTLRKNFERRVLPLEAAITASGRTKEEVITMASLVEREANGTED
                     RAMVAGILWRRFDEGKLLQVDAPFAAFLGKTSSELTRSDLLLDAPFNTYKHVGLPPVP
                     IANPGVAAIEATVYPTASPYLFYLHDADGTIHYARTYAEHLKNITRFLKNRK"
     gene            complement(4102..5376)
                     /locus_tag="VLB02_01345"
     CDS             complement(4102..5376)
                     /locus_tag="VLB02_01345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56710.1"
                     /translation="MYQSYSAIFTALFGQVAIFIALVVLLIMAWNLWLTYINTLFLNS
                     IKWVLLEVRPPKEVHKSPLAMELVLGALYQTGGVGTWYDRYWKGNLRYFFSLEIVSHE
                     GKVRFLVRTPDRFRKLIESQIYSQYPQAEVFEVEDYTKKIPDYKKGGPIELWGCRFEL
                     TKDDPYPIKTYVDYGLDKAVGSLDENQRIDPITPTLEFMGSLGAGEYFWFQILVQPDT
                     KRFSVKNKEGKEESGKDWKDKGREVIKELKGKLLEKDAEGKVMPGTSSQGQKLVIEAI
                     ERSINKLGFDCGIRALYLAEKASFNAGARIPSLTGILRQFNSSDLNGFKPAETTSFDF
                     PWQDPFGTRLETKKKYLLSDYKNRSYFYGGFSFDDVKKYFTSPGKSGKKPFILTSEEL
                     ATLFHLPGTVLETPSFERIEATKGEPPANLPI"
     gene            complement(5406..5954)
                     /locus_tag="VLB02_01350"
     CDS             complement(5406..5954)
                     /locus_tag="VLB02_01350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56711.1"
                     /translation="MADDKGAKPAAPAGHDPIVLIIGALLAITLIANLFGGKGKNQEP
                     QKTAPAAPQQQAVVEQQATYEQSCGLFVSSPEPLQKVSGGALLLEGMVGSCEWIPTGS
                     IGLYAQLIDSAGNLVSNYIAVRAEGGPGTPNAPILGTARPFSTHITLTGVPKTKTGFL
                     ILVNPDTTRATTKTVRIPVRFQ"
     gene            complement(5963..6991)
                     /locus_tag="VLB02_01355"
     CDS             complement(5963..6991)
                     /locus_tag="VLB02_01355"
                     /inference="COORDINATES: protein motif:HMM:NF016924.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HSE56712.1"
                     /translation="MSVISFKPKQITKRLLSQLQTRAHDIIVNRYGLGDDPEPKTLEA
                     IGKKYGITRERVRQIENAALAAIRKGEAFKAEHKVFSEIKDILSTLGVMVHEDELLSS
                     ISKDKSVQNHINFYLVLGDEFKKLKEDDAFKSRWTIDEEVSTIIHEALHTVYRNISDQ
                     ELLAEHDLVQRFLVELKQISEQYKNEEVARRWLSISKKIAKNPLGEWGPADSQNVKTR
                     GIKDYAYLMMKKHGSPMHFREVAKAISDTYGKKTHVATCHNELIKDPRFVLVGRGYYA
                     LAEWGYKPGVVRDVIRETLVKEGPLSKEEIIDRVLKERFLKRNTIIVNLQNNKHFKKL
                     DDGRYAAA"
     gene            complement(7056..>7614)
                     /locus_tag="VLB02_01360"
     CDS             complement(7056..>7614)
                     /locus_tag="VLB02_01360"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HSE56713.1"
                     /translation="SMLQSELGKEVSFTVDRKGVVHTITVTPKQDSVTKSYTIGIALE
                     PLETVQLPVHKALWYGLKDAWTITKNVVHALGVLVSGLVTKGGSGLNQFIGPVGLATA
                     VSEASQVGASYFFSFIALISLNLAVLNLVPFPALDGGRLLVLGLEAVTRRKFNKTVVG
                     IIHAVGFGLLILLMIFLTIQDVRHL"
BASE COUNT         2019 a         1857 c         1612 g         2126 t
ORIGIN      
        1 cactagtatt tgcattgata atttctattg ctggcacaaa tgtatcaatt tcatacacat
       61 tcgattttcc ttgtaccacc atcgtgatac gctcaagacc gctgccagta tcaacatttt
      121 tctgtacaag ctttccaata actttccccc cttttttctc gtactccatg aatacgtcgt
      181 tccagatttc aactacctca ccgcgatcat cagcagccat aaattcgtca tgagtcatgt
      241 caccaagccc ttctgtagtc gtatcgtaga acatttcagt atcgggacca cacggaccgt
      301 tatctcccgg gctccaccag ttattttttg ctggcaaaaa gtaaatccga ttttctggga
      361 catactgttt ccatagtgct gctgcctcag tatcacgcgg ggcattttca tcgccttcaa
      421 acacggtaac ataaaggcgt gccggatcga gtcctaaccc ttcctcttta gaagtcaaaa
      481 atttatagct ccactcaatt gcttctgctt taaagtaatc tccaagcgac cagtttccga
      541 gcatttcaaa aaaggtaaga tgtcgattat cgcccacctc atcaatgtct ccggtgcgaa
      601 cacatttttg gcagctcgcg agccgatttc cgagcggatg tttttttcca agcaggtatg
      661 gagcaagcgg ctgcatgccg gctgtattaa aaagtaccga cggatcgttt tcaggcacca
      721 aagaagcgct cggcaaaatt gcatgctggc gctttgcaaa aaaatctaaa aatcgtttcc
      781 ggatttctgc gctggtcatc atggttggta ctctaacaga aagaagataa aaggagaact
      841 ggcttactct tcaagctctg gatgctgctc agagagatta aaatttttat ttgagcgggc
      901 tgaaatcttg ctgatctgcc tttcaataaa ccagagcgca atgaaaataa aaagggtgag
      961 cactgttacc ataatcgcaa tctcgtaaag cccaaatccg gcagccagcc caataccggc
     1021 ggcaacccac aaaccgctgg cagtggttac accactcacc tggttattcc gatggaatat
     1081 tgctccaacg cccaaaaatc cggcagcagc aactacctgc gcagcaacac ggagcggatc
     1141 aaatgtttgg tttattgaaa actgcgccgc gacaatctga gaaatagtaa caaacagcgc
     1201 agagccgagc gaaacgaggg tgtacgtgcg cataccagcg gttttgcccg cgacaaagcg
     1261 ctcaagccca ataaccattc ccaaaaacat tgcaatagct aaattaacaa acacctgatc
     1321 tattcccata actgcggtat acatataccg agaagtatac cgagaaacgg taataaaaac
     1381 aaaaagtagc tatataatta ctcctccacc cacacacgta ttaccatcat agagcacgag
     1441 cgactgcgca ggagttgcaa ggactggttt ttcaaaaata attttttctc cagaaatggt
     1501 gcacgatacg agctcgccat ggtagcgcag ttgtgcctga taccgctttc ctgcctccca
     1561 tggaataatc tggtttatat tttttagtat tatttctgat acaagttcac tctcgggaat
     1621 gtagtgcgat gcaaccgtaa tagtattttt atcgatatct tttttaacta cgtagtacgg
     1681 accagcatcg gcgcgcacgg taacagtaaa accatgccgc tcgcccaggg ttacaaacca
     1741 cacaccgtca tgagtaccaa ttacgtttcc agaggtatcc actacagctc cctgccgcgg
     1801 agtaatatag tgccgtaaaa aatccttcat atcgacttcg cctaaaaagc agataccttg
     1861 gctatctttt ttctcggcgg taaaaagatt tgctttcgcc gcaatccggc gcacttcact
     1921 tttctgcaaa tgtccgatcg gaaacagggt atgtgccaaa tctgcttgag tgagcatcca
     1981 taaaaagtat gattgatctt ttgctgcatc tgctcctttt agcaactgat acccatcttc
     2041 tttttttacc tgtgcgtagt gcccagttgc gatgtagtct gcaccctggg cgagcgcgta
     2101 ctgcagaaat gaaccgaact taataatccg gttacaaagg acatcagggt ttggtgttct
     2161 gccggcgcgg tattcagcaa tcatgtggtc agcaacagct gttttatact ctgcttcaca
     2221 atctaaaaaa tggaacggaa taccgagcgc cgcgcacacc cgcattgcat cgcgcttttc
     2281 atccacccag gtacaaggta cccaatcagg tgcccaggtt ttaataaaca ccccatgtac
     2341 ttcgtacccc gcctttttaa gtaggtgtgc cgaaaccgac gagtctaccc cgcccgagat
     2401 gcccacaaat acttttttct gctgcttgct caccaaaaga tactagcacg agagaaactt
     2461 gacacaaaaa ataaaatgat tactataaaa agtcgggaat ctttaatctt taggtgatat
     2521 atggttggtc acctaacagc cccctacctt cgcggcgggg gtttttattt taaccccatc
     2581 aactttttaa acacctcgta ggtggcatac gcatctgaga aggcgctgtg cgcacgctta
     2641 ttttcaatac cgtagtattg gcagagcgcg ttgagtgaga atttgtttac atcgccctgc
     2701 cccgctaatt ttgcaaaagc gatagaaata gtatcgagct tgtgatagtg catatcgttt
     2761 gcaatgccgg ttttttcaaa tgcttttgaa ataaaaccgt aatcaaaggc aaggttgtgc
     2821 gcaatcataa tcgcaccgtt tgtttttttg gcaaatgttt ttattgcttc ctctaaggta
     2881 tatgcaaaaa gccacgcgcc ttcatcgtag ttatttactc gaagtgcctg ttggtcggca
     2941 gtttcaatat gctcgggctt taccttcaaa tcaacctgat ccaccacttc taatgctcca
     3001 tcggtgaccc gtgcaagaac cgcaccgagt tcgataattt cgtgcgtttc agtgctcaag
     3061 ccggtagttt caacatctat aaatgcaaaa gtttgattca acatatatct tttacttacg
     3121 attctttaaa aagcgcgtaa tattcttcaa atgctctgca tacgtacgcg cgtagtggat
     3181 tgtaccgtct gcatcatgta aataaaagag gtagggtgaa gcagtcggat acacggttgc
     3241 ctcaatagca gcaaccccag ggtttgcaat cggtaccggc gggagcccca catgtttata
     3301 ggtattaaat ggtgcgtcaa ggagaagatc gctccttgta agctcgctac ttgtttttcc
     3361 taaaaatgcc gcaaaaggag catcgacttg gagcaacttt ccttcatcaa atcgtcgcca
     3421 caaaataccg gcaaccatcg ctctatcttc cgtgccattt gcttcacgtt caaccaacga
     3481 tgccatggta ataacttctt cctttgttct acctgatgca gtaattgcag cctcaagcgg
     3541 tagtacacgt cgttcaaagt ttttccgcaa tgtggtaact actacatcag gcgttactga
     3601 cggaaaaaat gcgtacgtat caggaaacag gtacccttca tgctcttttg ccaagttcaa
     3661 aaaaagtgct ccgtcaaaat tggtaaatgt ttctgagaga cgtgctgcca tatcggtact
     3721 cgtgaaccct tcaggaaaag ttacccgctt gcggtcaata ccaaactgtc ttccggtaat
     3781 acggagcgcc atctgaaata ctgttgcagg tttggtgaag tagtactcac cctcgctaat
     3841 ggcgcgatca gtaccaaaca agagcatcca gaactcaagt aatcggcgcg attgtatagc
     3901 accctcagct tgcagctcgc ctgaaattga aaagaggctc tgccctgggt caatagtaaa
     3961 ctgatacggc accggaaacg attctggcgg acgatttaaa ccaaacaagt acccgccaac
     4021 tcccagcaaa aaaattagta tactgagtat gagaaggctg tgccgtctgc ggcgtggagg
     4081 aagtgtgtcg tattccatag gttatattgg taaatttgcg ggcggttctc ctttggtagc
     4141 ctcaatacgc tcgaatgatg gcgtttcaag cacggtacca gggaggtgaa agagtgtcgc
     4201 caactcctca ctggttaaaa taaaaggttt cttaccactc ttcccgggcg aagtgaaata
     4261 ttttttaaca tcatcaaaag aaaatcctcc gtaaaagtat gagcgatttt tgtagtctga
     4321 gagcaggtac ttcttttttg tttcaagccg ggttccaaaa gggtcctgcc atggaaaatc
     4381 gaaactggtg gtttcagccg gtttaaaacc gttgaggtcg cttgaattaa attgtcggag
     4441 aatgcccgtg agcgaaggga tgcgggcacc agcattaaac gatgcttttt cagcaagata
     4501 gagtgctcga ataccacaat caaaaccgag cttgttaata ctccgctcaa tcgcttctat
     4561 caccagtttt tgaccttgcg aggaagttcc cggcatcact tttccttcag catctttttc
     4621 gagcagcttc cctttcagtt ccttgataac ttcccgacct ttatctttcc aatctttccc
     4681 tgattcttct tttccttctt tattttttac gctaaagcgt tttgtgtcag gctgcaccaa
     4741 aatttgaaac cagaaatatt ctcctgcgcc gagcgaaccc ataaattcaa gtgttggtgt
     4801 aataggatcg atccgctgat tttcatcgag tgaaccaact gctttatcga gcccataatc
     4861 aacgtatgtt tttatcggat acggatcgtc tttggtgagc tcaaaccgac acccccacag
     4921 ctcgattggt ccaccctttt tgtaatcggg gatttttttt gtgtaatcct caacctcaaa
     4981 cacttctgcc tgcgggtact gcgagtagat ttgcgattca ataagctttc taaaccgatc
     5041 aggggtgcgg acaagaaacc gaaccttccc ttcgtgcgac actatttcga gtgaaaaaaa
     5101 gtagcggagg ttccctttcc agtagcgatc gtaccaggtt cctacaccgc ccgtttggta
     5161 cagtgcaccg agtaccagct ccatagcgag cggcgatttg tgcacctctt ttggcgggcg
     5221 tacttctagt agtacccact tgatagaatt taaaaagagc gtgttgatgt aggtgagcca
     5281 caaattccac gccataataa gcagtaccac cagcgcgata aaaatcgcca cttgtccaaa
     5341 caaggcggtg aatatcgctg agtatgattg gtacatagat agttaccagt atagcaaatg
     5401 aatgattatt gaaaccgaac agggatacga actgtttttg tcgttgctcg agtagtatct
     5461 ggatttacta aaataaggaa tcctgttttt gttttaggta caccggtgag cgtaatatgg
     5521 gtagaaaacg gtcgtgcggt gcctaaaatt ggcgcatttg gggttcccgg tccgccttcg
     5581 gcacgaactg ctatataatt gctcactaag ttgccggcag aatctatcag ttgtgcgtac
     5641 aagccaattg aacccgttgg tatccactcg cacgatccta ccattccttc aagtaacagc
     5701 gctccgcctg aaactttttg cagcggttcc ggtgagctca caaacagccc gcacgactgc
     5761 tcgtacgttg cctgctgctc aactactgct tgctgctgcg gtgcagcggg agcagttttt
     5821 tgcggttcct gattttttcc tttcccgcca aacaggttag caataagggt aatagccaaa
     5881 agagcgccaa taattaacac tattggatca tgtcctgcgg gagcagcggg ttttgcgccc
     5941 ttatcatctg ccatccctta tattatgcag cagcgtaccg tccgtcgtcc agttttttga
     6001 agtgcttatt gttttgaaga ttcacaataa tcgtgtttcg cttcaaaaat cgctctttga
     6061 gcacgcgatc gataatttct tcttttgaga gtgggccctc tttaacgagt gtttcgcgaa
     6121 tcacgtcacg aacgacacct ggtttataac cccattcagc aagtgcgtag tagccgcgac
     6181 caactaacac aaagcgcggg tctttaatga gttcgttatg gcaggttgcc acatgcgttt
     6241 ttttgccata cgtgtctgag attgcttttg caacttcacg gaagtgcatc gggctgccat
     6301 gcttcttcat catcaaatag gcgtagtctt taataccgcg tgtttttacg ttctgcgaat
     6361 cagctggacc ccattctccg agcggatttt ttgcaatctt ttttgagata gagagccagc
     6421 ggcgagcaac ttcttcgttt ttatactgtt ctgaaatttg tttcagttca accaaaaatc
     6481 gctgcactaa atcgtgctct gccaagagtt cctgatctga aatgttgcgg tacaccgtgt
     6541 ggagcgcttc gtggattatc gttgacactt cttcgtcaat cgtccagcgt gatttaaatg
     6601 cgtcgtcttc tttcagcttc ttaaattcat cgccaaggac caagtaaaag ttgatgtgat
     6661 tctgtactga tttatctttt gaaatactcg agaggagctc gtcttcatgt accattacac
     6721 caagggtact gagaatatcc ttaatttcgc taaatacttt gtgttcagct ttaaatgcct
     6781 ctcctttgcg gatagcagca agcgccgcgt tttcaatctg acgaacgcgt tcacgggtaa
     6841 tgccatactt tttgccgatt gcctcgagtg tttttggctc gggatcgtcg cccaacccat
     6901 agcgattcac aataatatcg tgggcacggg tctgcaactg cgagagcagg cgcttggtta
     6961 tttgttttgg tttaaagctg ataactgaca tggtgtaata attattttct actaactgat
     7021 tttatcatgg atgcaaaaat tgtcaacaaa cccctttaca gatgtcgaac gtcctgaata
     7081 gtgaggaaaa tcataagtaa aatcaggagt ccaaacccta ccgcatgaat tatccccacc
     7141 actgttttgt taaattttcg acgagttaca gcttcaagcc cgagcaccaa gagtcgccct
     7201 ccatcgagcg ccggaaacgg caccaaattg agcactgcca agttaaggga aataagtgca
     7261 ataaacgaga agaaatacga agcaccaacc tgtgaagcct cggatactgc cgttgcaagc
     7321 cccaccggtc caataaactg atttagccct gatccaccct tcgtaaccaa cccgctcacc
     7381 aaaacaccca gtgcatgcac gacgtttttg gtaatcgtcc acgcatcttt gagcccatac
     7441 cacagcgctt tgtgcactgg tagctgcact gtttcaagcg gctcaagggc aatacctatg
     7501 gtgtagcttt tagttaccga atcttgcttt ggtgttacgg taatggtgtg cactacccct
     7561 tttcgatcaa cagtaaaact aacctctttt ccaagctctg actggagcat gctc
//
LOCUS       DATIBQ010000046         2739 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U16294 k141_708,
            whole genome shotgun sequence.
ACCESSION   DATIBQ010000046 DATIBQ010000000
VERSION     DATIBQ010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935789
            Sequence Read Archive: SRR25158331
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2739)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:58:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 637
            CDSs (total)                      :: 603
            Genes (coding)                    :: 601
            CDSs (with protein)               :: 601
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2739
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_708"
                     /isolate="SMAG_U16294"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..807
                     /locus_tag="VLB02_01365"
     CDS             <1..807
                     /locus_tag="VLB02_01365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56714.1"
                     /translation="TDSTTSSATGSTGLALYDWLVAFDEPITSDTTAPTVSSITSTTA
                     SGTYTPGNTINITVTFSEAVTTTGTVTVNLNSGGSCTITASSSTTASCTYTVLSGQTA
                     NPLTVSSISGTVQDASSNTMTNSTPVTNLSASKTIVIAAHGGGGNSGSKGRKPLSLVP
                     TPVPAALIPAVTAPDTPHRLTCSSLDESLTLRVGSSQTAQIATLQSFLKSTVAPWLRV
                     SGVFGPYTTLAVNKFQLRYPNETYRLANRKAPTGIAGYYTLRLINSFLCS"
     gene            834..1271
                     /locus_tag="VLB02_01370"
     CDS             834..1271
                     /locus_tag="VLB02_01370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56715.1"
                     /translation="MHIAKNIVIVIVGLILVSIAYGFVKKGAEPQTPLAEPAPLPATE
                     TTPALITAAIGQSVTVGAVSIMPTAVLEDSRCPANVQCIWAGRVRVQVAVTNGTVQQL
                     IEITLGESLVVAGKTIALVAVRPEPLAGEKPASAAYEFDFKVQ"
     gene            complement(1317..1562)
                     /locus_tag="VLB02_01375"
     CDS             complement(1317..1562)
                     /locus_tag="VLB02_01375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56716.1"
                     /translation="MKKAKQFIISSTFALFLSSSVITSAASYPTTLHKQAVEAAIAAT
                     EPVDAPLYLFVPKEDRYQKKMHYKQMLRTRGEKRMYR"
     gene            complement(1601..1921)
                     /locus_tag="VLB02_01380"
     CDS             complement(1601..1921)
                     /locus_tag="VLB02_01380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56717.1"
                     /translation="MRSGVQRQLQKYLSLVLVGYFAVTTFYGSPEQLIQSPSELSGIC
                     TEAGISNTAGTTLSKESSFSCILPEHSLIIAPLGAPTLATIVAEDHISTPQTHSARAP
                     PVLL"
     gene            2017..2092
                     /locus_tag="VLB02_01385"
     tRNA            2017..2092
                     /locus_tag="VLB02_01385"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2050..2052,aa:Thr,seq:ggt)
     gene            2187..2381
                     /locus_tag="VLB02_01390"
     CDS             2187..2381
                     /locus_tag="VLB02_01390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56718.1"
                     /translation="MPRPNIYQNWKLPNHEANKTNSWETLEKKEAATNRKKKIPESEE
                     LERTMEAEREENLEMFSDPD"
     gene            2486..>2739
                     /locus_tag="VLB02_01395"
     CDS             2486..>2739
                     /locus_tag="VLB02_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HSE56719.1"
                     /translation="MEIRNIAIIAHVDHGKTTLTDHMLRQQGEIEEGASMDSNAIERE
                     RGITIYAKNTAVNYKGTKINIVDTPGHADFGSEVERVLRS"
BASE COUNT          771 a          623 c          630 g          715 t
ORIGIN      
        1 accgacagta ctacttcaag tgcaaccgga agtactggtt tggcgctcta cgactggctc
       61 gtagcatttg atgaaccaat tacttccgat acaaccgcac caactgtttc ttctataact
      121 tcaacaacag cgagcggtac ctatacgccg gggaacacaa taaatattac agttaccttc
      181 tcagaggcag ttactactac ggggacagta acggtgaatt taaattctgg cggctcatgt
      241 accattactg caagcagtag tacaacagcg agctgtacat acactgttct ctcgggacag
      301 acggcaaacc cgctcactgt ttcaagtatt tcgggaacgg tacaggatgc gtcatctaat
      361 actatgacca atagcacacc ggtaacaaac ctctctgcat caaaaacaat tgttattgct
      421 gcacatggcg gcggaggtaa ctcgggaagc aaggggcgta aaccgctctc gctcgtgcct
      481 accccagtgc ccgctgcact tattcctgca gttactgcgc ccgatacccc gcaccgcctc
      541 acctgctctt ctcttgatga atccctcacg ctccgggtgg ggagcagcca gacagcacag
      601 attgcaacac tgcaatcttt tttaaaaagt actgttgcac cgtggcttcg ggtaagcggt
      661 gtgtttggtc catacaccac gctggcagtg aataaatttc aattgcgtta tccaaacgag
      721 acgtaccgat tagcgaatag gaaagctccc actggtattg ccgggtatta caccctccgg
      781 cttattaata gttttctctg ctcgtaaatg gttttttgtg gtatacctat cctatgcata
      841 ttgctaaaaa tattgtcatc gttattgtag gacttattct tgtttctatt gcatatggtt
      901 ttgtgaagaa gggagcagaa ccacaaactc ctcttgcaga acctgcaccg ctgcctgcta
      961 ctgaaacaac accagctctt attactgcag cgatcgggca aagtgttacc gtaggcgcag
     1021 taagcattat gccaacggca gtactcgaag atagccggtg tccagcaaac gtacaatgta
     1081 tctgggcggg cagggtgcgg gtgcaggtag cagttacaaa cggcactgtc cagcagctta
     1141 tagaaattac tcttggtgaa tcactggtag tagccgggaa gactattgca ttagtggctg
     1201 ttcgtcctga accgctagct ggagaaaaac cagcaagcgc tgcgtatgag tttgatttta
     1261 aggttcagta atccactgta tacaaaaccc cgaccactat ggtcggggtt ttgctactat
     1321 cgatacattc gtttttctcc tcttgttcgc aacatttgtt tgtagtgcat ctttttctgg
     1381 taccgatctt cttttggtac aaacaggtac aaaggagcat ctactggctc tgttgctgca
     1441 atggcagcct caaccgcctg tttgtgcaga gtggtggggt aggatgctgc cgaggtgatt
     1501 acggagctcg agaggaaaag cgcaaacgtt gaactaataa tgaattgttt tgctttcttc
     1561 atatgttctt ttttactcta ctaggtgaat aataagtaac ttacagaagg actggcggcg
     1621 ctcgggcgct atgggtttga ggagtgctga tgtgatcctc agcgacaatt gttgccagtg
     1681 tcggcgctcc aagcggcgca ataataagag agtgttcagg aagaatacaa gaaaacgaag
     1741 attcctttga aagggtggta ccagcggtat tactaatacc tgcttcggta catatccccg
     1801 ataattcaga gggggattga atgagctgct cgggactgcc gtagaaagtg gtaacagcaa
     1861 aatagccaac cagcaccagg ctgaggtatt tttgaagctg ccgctggact ccacttctca
     1921 tacttttact gtataccgct ttaaaaaaat ggcaagagca gaaaaaccct tgaaaaaata
     1981 aggtttttat actatattgg ttcggtcgtt atagtagccg acgtagctca gttggtagag
     2041 cacgcccatg gtaagggcga ggtcatcggt tcaatcccga tcgtcggctc catataaaaa
     2101 tccccagact atgtggggat ttttatatgg agccatttga ctttttactg caatgtatgt
     2161 aaattgtaaa aaaatccatt tttacaatgc caagacctaa tatctatcag aattggaaac
     2221 tccccaacca tgaagctaat aaaacaaaca gttgggagac tttagagaag aaagaagcag
     2281 caacaaaccg gaagaaaaaa attcctgaat cggaagaatt agaaagaacc atggaagcag
     2341 aacgcgaaga gaatcttgaa atgtttagtg atcctgatta aaaagtaata aaaaaattag
     2401 aaagccagcc cttaccggct ggctctttct tttatatgta gcccttcttt tttatacata
     2461 ttcgtgtata attctccgtt actgtatgga aatcagaaat atagctatta ttgcccacgt
     2521 tgaccacggc aaaaccaccc tcaccgacca catgctccgc cagcaaggcg agattgaaga
     2581 gggcgcttct atggactcaa acgctatcga acgagagcgg ggaattacca tttacgctaa
     2641 aaacacggca gttaattata agggtaccaa aataaacatc gttgatacgc ccggccacgc
     2701 cgactttggc agcgaggttg agcgcgtgct ccgctcgat
//
LOCUS       DATIBQ010000047         2833 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U16294
            k141_2017218, whole genome shotgun sequence.
ACCESSION   DATIBQ010000047 DATIBQ010000000
VERSION     DATIBQ010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935789
            Sequence Read Archive: SRR25158331
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2833)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2833)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:58:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 637
            CDSs (total)                      :: 603
            Genes (coding)                    :: 601
            CDSs (with protein)               :: 601
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2833
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2017218"
                     /isolate="SMAG_U16294"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            84..392
                     /locus_tag="VLB02_01400"
     CDS             84..392
                     /locus_tag="VLB02_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HSE56720.1"
                     /translation="MSDKEFLELLVKELVENPGDVKVDRTVDEMGVLLTLSVNPADMG
                     KIIGRSGNTAKAIRTLLRVVGMKHNARVNLKINEPEGGAKREVEAANIDETMDDLKGI
                     "
     gene            483..1121
                     /gene="trmD"
                     /locus_tag="VLB02_01405"
     CDS             483..1121
                     /gene="trmD"
                     /locus_tag="VLB02_01405"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HSE56721.1"
                     /translation="MKFHFLTIFPEMIRAYTDESILKRAQEKKLIAVAAVNPREYTKD
                     KHRKVDDRAYGGGPGMVMLAEPLLRATEKLKLKGKKRKVIMLSPTGKQFTNELAKKWA
                     KQYSDIVFICGRYEGVDDRVRKILKAEEVTVGPYVLTGGELPALTIADAVARQLSGVL
                     GKGESIEENRTASPRTYTRPEVLTWKKKNYKVPKVLTMGNHKDIEEWRKSQQ"
     gene            1643..>2833
                     /locus_tag="VLB02_01410"
     CDS             1643..>2833
                     /locus_tag="VLB02_01410"
                     /inference="COORDINATES: protein motif:HMM:NF016450.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HSE56722.1"
                     /translation="MSKTKAVSGARPKKYFSKYKKPLVEFPNLIQHQLDSFEWLVKHG
                     LKNVFKDFTPISDYSGKKFQLDFESFSLVEPKIDEHYAKENKLSLEAQLKTRVRLTNK
                     QTASEKEQEIFLADIPVMTSHGTFIINGVERSIIPQLSRSSGVFFTLNELRGKRYFGA
                     KIIPNRGVWIEIESEADGAVYVRIDKKRKFPVTSLLRVLGFTTDADILKAFTGDVAPQ
                     IIKQSLEKDPAKTVEESYVEIYRKLRDGDMATADNARDFIKNLFSEERYDLSSVGRFR
                     FNKRFELDMSDKEMERKTLSAADLAVIVNHIITLNITPGAHEDDIDHLGQRRVRFVGE
                     MLSQKIFMGMTQIKRNIQDRMSTIDTATALPIQIINQRPLQARIKEFFTTNQLSQFMN
                     QENVL"
BASE COUNT          858 a          584 c          668 g          723 t
ORIGIN      
        1 aggtgttttt tctttaaaaa gtttatatac ttaccgtagg tgccagtcgg tactattact
       61 actaaagatc agaaaagaaa tatatgtcag ataaagaatt tttggaactg ttagtgaaag
      121 agttagtgga aaatccaggc gatgtaaaag tggatcgcac ggttgatgaa atgggtgtac
      181 tcctcacgtt gtcggtgaat ccggcagata tgggtaaaat tatcggtcgc agcggcaaca
      241 ctgccaaggc gatccgcacc cttttgcggg tagtggggat gaagcacaat gctcgggtaa
      301 atcttaaaat caacgaaccc gaaggcggcg caaagcgcga agttgaagcc gcaaacatcg
      361 acgaaacaat ggatgatttg aagggaatat aattatccgc actcaaaaaa ggtcataaaa
      421 accaccgaga aataggtggt tttttgactt tctgcctact ccttactagt atctagatac
      481 ttatgaaatt tcattttttg actatttttc ctgagatgat ccgggcgtat acggacgaat
      541 cgatactgaa gcgtgcgcag gagaaaaagc tgattgcggt tgcggcagtg aatccgcgcg
      601 agtacacaaa agataaacac cgcaaagttg atgaccgtgc ctacggcgga ggaccaggaa
      661 tggtgatgct tgcagagcct ctgcttcggg ctactgaaaa actgaaatta aaaggcaaaa
      721 agagaaaagt aattatgctc tcaccaaccg ggaagcagtt taccaacgaa ctcgctaaga
      781 agtgggcaaa acagtacagc gatattgtgt ttatttgcgg tcgctatgaa ggagtagatg
      841 atcgggtgcg aaaaattttg aaggcagaag aagtaacggt tggaccgtat gtacttacag
      901 gtggtgaatt gcccgcgctt acgattgccg atgccgttgc tcgacaatta tctggcgtgc
      961 tcggaaaagg ggaatcaatt gaggaaaatc ggaccgcttc accgcgcact tatacccgac
     1021 cagaagtgct tacttggaaa aagaagaact ataaagtacc gaaagtgctc accatgggta
     1081 accacaagga tatagaggaa tggcgcaaga gccagcagta gcatttgact ttatattttt
     1141 tttatgatat agtgtagaaa attgatcata tggggaataa tctatctttg aggaagtgga
     1201 gaaagttata cataaccaac gaaattgagc ggaggagaaa aattaatcaa aaaataaaaa
     1261 gtgattttct tcttttaatg aagagtaaaa agtcagtttc agttaaggtt gtttctgatc
     1321 cactaccacc gataccggta ttgataccga taccgacgct accaaaaaag acagcggaca
     1381 tattccatct ctaccccggt ggagagttgg taaccgcaga acggtggtaa gagagagaac
     1441 gttttaataa gcccgaggca gtaccgctcg ggcttatttt agtggatttg acaaaaatct
     1501 gctcttactc tacaattccc gagctaaatc tctgtatgag agtagccgga aatttccttt
     1561 tataaacgag cgatcgtcct attcagccgc ccagcacaat tgggtattga ataggcgtat
     1621 ttttatttga gtaaaaacaa taatgagtaa aacaaaagca gtttcgggcg cccgcccgaa
     1681 gaagtacttt tcgaagtaca agaagcctct agttgaattc cctaacttga tccagcacca
     1741 gctcgacagt tttgagtggc tggtgaagca cggactgaag aatgtgttta aagactttac
     1801 tcccatctct gattactcgg gaaagaagtt tcagctcgat tttgagtcgt tctcactcgt
     1861 tgagccaaaa attgacgaac actacgcaaa agaaaacaag ctctcgcttg aagcgcagtt
     1921 aaaaacacgt gttcgactca cgaacaaaca gacggcttct gaaaaagagc aggaaatctt
     1981 tcttgctgat attccggtga tgacctcaca tggcaccttt attattaatg gtgtcgagcg
     2041 ttctattatt ccacagctct cgcgttcatc gggggtgttt ttcacgttga acgaactgcg
     2101 cggcaagcgc tactttgggg caaaaattat tcccaaccgc ggtgtctgga ttgaaattga
     2161 atctgaggct gacggtgcgg tgtatgtgcg tatcgacaaa aagcgaaagt ttccggttac
     2221 ttcgctcctt cgagttcttg gttttactac cgatgctgat attttaaaag cgttcactgg
     2281 tgatgttgct cctcagatta ttaaacaatc gctcgagaaa gatccggcaa agacggttga
     2341 agaatcttac gttgaaattt accgcaagct ccgcgatggc gatatggcaa cagctgacaa
     2401 cgctcgcgat tttattaaga atctcttctc tgaagagcgg tacgatcttt catcggtggg
     2461 tcgctttcgc ttcaacaagc gcttcgagct cgacatgagc gacaaagaga tggaacgcaa
     2521 aacgctctcg gctgccgacc tcgcagtgat tgtcaatcat attattacgc tcaacattac
     2581 tccgggcgca catgaagacg atatcgatca cctcgggcag cgccgggtac gttttgtggg
     2641 tgaaatgctc agtcaaaaaa tctttatggg tatgacccag attaagcgca acattcaaga
     2701 ccgcatgtcg acaattgata cggcaactgc gctcccaatt caaatcatta accagcgtcc
     2761 gctccaagct cgaattaaag aattttttac caccaaccag ctctcgcagt ttatgaacca
     2821 agagaacgtg ctc
//
LOCUS       DATIBQ010000048        16963 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U16294
            k141_1697042, whole genome shotgun sequence.
ACCESSION   DATIBQ010000048 DATIBQ010000000
VERSION     DATIBQ010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935789
            Sequence Read Archive: SRR25158331
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 16963)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16963)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:58:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 637
            CDSs (total)                      :: 603
            Genes (coding)                    :: 601
            CDSs (with protein)               :: 601
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16963
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1697042"
                     /isolate="SMAG_U16294"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1038)
                     /locus_tag="VLB02_01415"
     CDS             complement(<1..1038)
                     /locus_tag="VLB02_01415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56723.1"
                     /translation="MQNPVALRFYNVSFVLLLCTCALLPLLFLPATIGSFAAVKYTLL
                     SLGVLLTGTTWIISQFVEGTMQFPRHRALLGLFIVAALSFLSSLFSPNVPVSLWGNIF
                     NFDSFSTIAILALFAFLVSLFAREQRRLVKLFLTMFFVSAGTVLLQIILFLLHKVPFV
                     IKYFDHVASQGTLVGTWVDFSFFTAFTFILSILMIEVLAPKGLFRTLSLGGVVVSLLA
                     LVFLNFNTAWLFAVVSSLLVFVYKSSVERALIARDQHAEEHSEYRQRFPAVSFISLLI
                     GLFFLLAGSSLGGAISRFSGLSFSDIRPSLSATLTIARPVLAKDPFFGGGPARFSELW
                     DLHKSSEINKTV"
     gene            complement(1084..3639)
                     /gene="leuS"
                     /locus_tag="VLB02_01420"
     CDS             complement(1084..3639)
                     /gene="leuS"
                     /locus_tag="VLB02_01420"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011669510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HSE56724.1"
                     /translation="MKRYNHQSLEKKWQKEWERKGIYKAESTSRKKKMYVLDMFPYPS
                     GTGLHVGHPRGYIASDIYARLKRMQGFNVLHPMGFDSFGLPAEQYAIQTGKHPGPFTE
                     TLIRRYKQQLSIMGLSYDWSREVATHRPDYYKWTQWIFLKLFDAYYDAKKNKARATKE
                     LVKEFEKKGNKNSTAVCDTDTPIFSAKEWKSFSKPEQEKVLMKYRLAYEGYSEVNWCP
                     ELGTVLANDEVVDGPNGTLLSERGNFPVEKRKMRQWFLRISAYAERLLQGLEAINWSL
                     AIKEIQRNWIGKSEGSEIPFILSNGETVTVFTTRADTLFGATYLVLAPEHALVQTLRP
                     YIRNWEMVEKYIHEVANKPDEVRSAEAKEKTGVVLEGVTVINPATKEQIPVWIADYVL
                     ASYGTGAVMAVPAHDERDFAFAKKYNLSIRHVIFKDGTVAETEEKPFCEYGITLNSGE
                     FSGLTSEEAKVKITKVVGGKIVTKYKMRDAVFARQRYWGEPIPLVHKSDGTIVPLAEN
                     KLPLKLPNVKSYKPSGTGESPLANIKEWVQKGYETNTMPGWAASSWYFIRYMDPKNKK
                     AFASKQAIKYWKNVDVYIGGTEHATGHLLYARFWHKFLYDLGLVPTEEPFQQLKNQGM
                     ILGADNRKMSKRWGNVVNPDDVVKTYGADTLRLFESFIGPFEASLPWSTEGIIGSRRF
                     IERIWKLAGTPFAVKPSEQNEKLLHKTIKKVTEDIESFNFNTAVSAMMIFTNSIEGAL
                     TKKQFGIFVRLLAPFAPYVAEELWQQLGEKKSIHLSVWPVWDKKKVIEDTVTIMVQVN
                     GKIRGQFEAPVTIAPEAAQKIALGLEKVAPHLAGKKVKKVISLPQKKIVNIVV"
     gene            complement(3694..4704)
                     /locus_tag="VLB02_01425"
     CDS             complement(3694..4704)
                     /locus_tag="VLB02_01425"
                     /inference="COORDINATES: protein motif:HMM:NF039189.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5671 domain-containing protein"
                     /protein_id="HSE56725.1"
                     /translation="MTETKKLSPLFFFLSIGVLVALIVSVTGLLQLLFEAFNHTFPDV
                     LTDSYRYGYYSYSFEKLRNSLALLIIVFPVFLTLMHFWLRAAKQHLSHWDEVLRRWAI
                     YLVLFLASVMIIADLVTLVRYFVSGEITVRFILKVAVTLIAASAVGWYYIRELKRSVN
                     HTDSKARWFAIGSSVLVLVAIIYGFTVIGGPGSQRKLRLDQRRIDDLQSIQWQVISFW
                     QQKETVPETLVDIATPISSYLVPQDPEFQKGNAYEYRKFSDLKFELCATFAAPMPEGW
                     VPEGQGGDMPVPMMDRAETSVGYPGISNINNNWDHDAGRTCYQREIDPELYPPFPKPE
                     KR"
     gene            4805..5710
                     /locus_tag="VLB02_01430"
     CDS             4805..5710
                     /locus_tag="VLB02_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HSE56726.1"
                     /translation="MRPLKITKSITNREQDSVEKYLSEIGKVQLIEPEEEAVLARKIK
                     EGDQAALDKLVKANLRFVVSVAKQYQNQGLSLSDLINEGNLGLIKAAQRFDETRGFKF
                     ISYGVWWIRQSILQALAEQSRLVRLPQNKIALTNKIQKVYLELQQEFEREPSAEELAE
                     LLEIEVGRVDAASINSRSFSLDGPLPEGDGDTSFYEIYPDDILSPDAELEKESLQRDL
                     EEAFKRLSPKQQEILKKAHGIGYDFSWSHESIADEYNLTKERVRQIIEKSEDRLATCK
                     NLRQYRPIDMKKDVKKKKIRKKVKI"
     gene            5789..6256
                     /locus_tag="VLB02_01435"
     CDS             5789..6256
                     /locus_tag="VLB02_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56727.1"
                     /translation="MKKPSTVREKIERRADRATEWIGSTKSVLVHSIVFAVAFLLPLF
                     GADFDRVLLILTTAVSLEAIYLAIFIQMTVNKNTEDIEEIQEDVEEIQVDVDEIQKDV
                     DEIQEDIEDIEEVQGKEEIEELQKQKKDYDLLSDMQKTLKKMSEEIEDIKKRT"
     gene            complement(6643..7644)
                     /gene="ychF"
                     /locus_tag="VLB02_01440"
     CDS             complement(6643..7644)
                     /gene="ychF"
                     /locus_tag="VLB02_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013059916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HSE56728.1"
                     /translation="MGLSIGIVGLPNVGKSTLFNALTKKGVPAENYPFCTIDPSVGIV
                     AVPDERLQKLSDFSKSAKTVPAAIEFVDIAGLVKGAAQGEGLGNKFLSNIREVDAILE
                     MVRVFEDKDIIHVANKIDPLDDISVINMELVLADMETVSKRRGNIQKEVKRGDKQAVL
                     EDGALAKLLPILEEGKLAATVSLDEKEVPLVKQLGLLTMKPILYALNASEVAENVIEG
                     VKQKIPGAHVVIDAVFEKGFDDLIKAAYELLGLETFFTTGEDETRAWTIKKGSTAPIA
                     GTAIHTDFKEKFIRAEVVYWKDLLDAGSYAEARSKGLVRIEGKEYVVKDGDVIEFKI"
     gene            complement(7651..7887)
                     /locus_tag="VLB02_01445"
     CDS             complement(7651..7887)
                     /locus_tag="VLB02_01445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56729.1"
                     /translation="MFGLKNLLMKKMLKNQLKGVPEAEQERIIAVISKNPDFFKKIGE
                     EIKMKMKQGKNQMAATMEVMRDHQAEMQKLMQEK"
     gene            7971..8312
                     /locus_tag="VLB02_01450"
     CDS             7971..8312
                     /locus_tag="VLB02_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017494667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HSE56730.1"
                     /translation="MKEFDLEKRTFLFAQAIRTFIKELPKTITSFEDAKQLVRSSGSV
                     GANYIEANEALSKKDFLMRIKICRKEAKESIYWLQLIEIPEHLLEKKKQLIAECTELL
                     KIFSAIVSKSS"
     gene            8375..8956
                     /locus_tag="VLB02_01455"
     CDS             8375..8956
                     /locus_tag="VLB02_01455"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008482017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HSE56731.1"
                     /translation="MSKTLAEIHEYWREHCTCELKKTATQAVFGDGNPQAEIVFIGEA
                     PGKNEDLEGRPFVGAAGKFLAEMLESIKMQRSDIYITNIVKYRPPNNRDPLPEEIEAC
                     KEWLYEELNTIKPKLIVFLGRHSMSHFFPGEKISIVHGKLLIKKIPGIETNNFLPLYH
                     PAAALYNGGMRETLLQDFKKIPLALKKIQGTEK"
     gene            complement(8957..9712)
                     /locus_tag="VLB02_01460"
     CDS             complement(8957..9712)
                     /locus_tag="VLB02_01460"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HSE56732.1"
                     /translation="MKNNSTSWSSVAPWYDEYLETSADSYQAKVIAPNLLRMLELKKG
                     EAVLDVGCGQGYFSRLLAEAGAAVTGIDISEELITCAKEKGGTARYFAAPAHDTKLKA
                     YSFDTTLIVLALENIKNIDQTFAEIARVLKKEGRVVLVMLHPAFRIPQFSDWGFDTKK
                     NLQYRRVDKYLSEAAIEISQNPFKGTKEAKTVTYHRSLQWYMKVFKKHGFSITAMEEW
                     ISHKKSAPGIRQEAEDRARKEFPMFLALEIGKK"
     gene            complement(9702..10964)
                     /locus_tag="VLB02_01465"
     CDS             complement(9702..10964)
                     /locus_tag="VLB02_01465"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HSE56733.1"
                     /translation="MNTGSKPRILIVGGGFGGIETALGLKDVADQYQVTLLSDKTYFE
                     YYPALYKVVTGALPTEVSIPLWMIFDQTAITVEHDRAVTFIPEEKRVIGESGSHYEYD
                     YLVLAIGSQTNYFSIPGLAEHSLSFKSVHEALRLKNHFYRLFTQGNGVKEELVSLLHI
                     VIVGAGPSGVELAGDLKEYLQTLAEKHHVDPSLVTIDLIESNPRVLPSLPESVSRMAE
                     ARLRKLGINIFTNRTLMQKDIEEVHLRDMQMKTETVIWTAGTKINEQFAKIPALAFSA
                     RKRVVVDEYLRVPELSNFFIVGDGAETPYSGLAQTAIHNGKYVAQAIKKVITGKTLTP
                     YNPHKGAYIIPIGSRWAILAMNRFRAYGFLPWVLRTLVDVRYYAHILPFRRLWYVLRE
                     GKKYGGIAGGCKNEEECLPPEPPKHHEK"
     gene            11092..11622
                     /locus_tag="VLB02_01470"
     CDS             11092..11622
                     /locus_tag="VLB02_01470"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HSE56734.1"
                     /translation="MNPHTPGNPDLEKLFFELFEAYNDTIFRFVYFQLRSREQALDIT
                     QEVFMNTWRYLAQGKQLENPEAFLYRSARNAVIDFYKKKKSLSLDGLMEDGFEPGGDD
                     ELNLHLKQGDLNLIAEAVRMLDPKDQQILLLRYTEELPIEEIAEQFGKSTNAMTVQIH
                     RIIQKLKKIYETTYGR"
     gene            11612..12448
                     /locus_tag="VLB02_01475"
     CDS             11612..12448
                     /locus_tag="VLB02_01475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56735.1"
                     /translation="MEDNLKQLHTLKRITLTATEQHAMRSRLAHYVQETPIETQSMFE
                     VFVSHGLRVALSSFLFFILIGGSVSALANSSLPGDPFYNVKLNVNEKVQEWLTKNTPE
                     QKVAFQQERIENRLKEIQLLAETKTLTKEKRAVAEEALSKHTTKFSAELGELSLKQPE
                     AALKATAKLEETLRVQKDALTLAPEAAASGQNEAVLEVVDSALASVVQEEQRIIQAEL
                     SKVEDELLNEDPIDDIDSALAPSDEAPADIEEKQSSSEHKTDEVSAKNESPIPPVTPH
                     EP"
     gene            complement(12429..13802)
                     /locus_tag="VLB02_01480"
     CDS             complement(12429..13802)
                     /locus_tag="VLB02_01480"
                     /inference="COORDINATES: protein motif:HMM:NF013125.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HSE56736.1"
                     /translation="MSNSLSIGIILAAFPAVAFAAVDVTEIAWMGTAESQYGEWIELY
                     NNGTDSVSLDGWKLYQDGGSALLFSLTKTIPAGGYLVVERTTPSVPDPLPAISDEAGT
                     FGGSGLANTGEHLVLKDASGTTVLELPFQAGWPAGDAATKETMQKKGSGWITAAPTPK
                     AATVDVGTNTNTEHVDDENTPETETSKTNTISGGGSTEPKIIWKPNISFIYPKQAFTG
                     VPVRITATVTEESGVTRAGHFVWNMGEGRVIEQQLLEPVTYTYQYPGEYVITLAYTRR
                     EAITSDAELVGQFSLSVTNPVLTLERDSAGALFIKNTSTENIDLYQWKLLFAGNQYEF
                     PRYSIVKAKNRLPVSGALVGVASFSELPTLANPSSIAVAGPQAITVASVPKTRSVAGV
                     QFQKSVALPEPAVTASEQPLAANALGAVSTTQNNKRTWGVLALGALAAIAIALFLLLE
                     RFKAHEE"
     gene            complement(13795..15825)
                     /locus_tag="VLB02_01485"
     CDS             complement(13795..15825)
                     /locus_tag="VLB02_01485"
                     /inference="COORDINATES: protein motif:HMM:NF014581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system DNA-binding
                     domain-containing protein"
                     /protein_id="HSE56737.1"
                     /translation="MAFDPEQVTYFAETDFRNQKTRFGIKAKDRPRHMYVIGNTGVGK
                     STLLENMAIQDVKNGNGIGILDPHGSFVEKILDYIPQHRIKDVIYFAPFDTNYPVSFN
                     VLEDVGADKRHFVVSGLMSTFKKIWVDAWSARMEYILNNILLALLEYPGATLLGVNRM
                     LADKDYRNKVVANATDPGVKAFWLDEFAKYGDRYMQEAGAAIQNKIGQFSANPLIRNI
                     IGQPKSSFNFRTAMDEGKIVVMNLSKGRIGEQNTSLLGSMLITKIYLAAMSRADVHPT
                     KLAELPNFYFYVDEFQSFANETFANILSEARKYKLNLIIAHQYVAQMEESVRDAVFGN
                     VGTLVSFRVGPLDAELLEKAFSQHFTPNDMVSLGMGQIYLTLMIDGTGSKPFSAKTFL
                     PIPFPDISFRNDVIEYSRSVYAAPKDEVEKGIGDWYIKEEAPVRERPPRQEYQQQAAP
                     QQSQFRPQPQQSYVSRPPLREQPRPPISTPPVQQFQKPPAPAWQKPPTPEQPKEHQPF
                     KAALGTMQPPVQNESVQKKEPPKESLQSFLATLDLEKKPEQPSFQAAHRAPVLQPSPR
                     PAASPPAPPPSKPAAQEKQEQTSAPAAPADHNPFLPNLRPKPARVLDRAATEDSRAML
                     RTVLAKTQTPKPAEDQQPPVHTAAQPPPAAAGSKEKEDVSEVSHEVLKKVLE"
     gene            complement(15841..16584)
                     /gene="rlmB"
                     /locus_tag="VLB02_01490"
     CDS             complement(15841..16584)
                     /gene="rlmB"
                     /locus_tag="VLB02_01490"
                     /inference="COORDINATES: protein motif:HMM:TIGR00186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HSE56738.1"
                     /translation="MKSEKLYMYGKHALTEALAHMPQAVKKVFLAEAIGGDIRKLLDT
                     HNIPYVPLKEEEKRQVAADAAHQGVIAVVDTEKLQQPFDTFINHLSVTADTCLVLLDE
                     LQDPHNVGAIIRSAAGFGATAVLIPEHNQAPITAAVIKTSAGMAFRIPLVRIGNVNTT
                     LRALKEKGFWVYGLAMEGSKELGTESFDAPTVLVVGNEGEGIRQKTLELCDVTLRIPM
                     HPRTESLNAAVSAAVVLYQWSTTHTAVLK"
BASE COUNT         4647 a         4025 c         3743 g         4548 t
ORIGIN      
        1 tacagtttta tttatctcag acgatttgtg aagatcccag agctcagaga atcgagcggg
       61 tccgccgcca aagaaggggt ctttagcaag caccggacgg gcaatagtga gtgttgctga
      121 aagtgagggg cgaatatcag agaagctcaa gccagaaaat cgcgaaattg ccccgccaag
      181 agatgagcct gcgaggagga agaagagccc gataagcaat gatatgaaag aaacagcagg
      241 gaatcgttgt cggtactcgg agtgctcctc agcgtgttga tctcgagcga tgagcgctcg
      301 ctcgaccgat gatttatata caaacaccaa gagtgatgat acaaccgcaa agagccacgc
      361 ggtgttgaag ttaagaaaca cgagtgcgag caacgacact accaccccgc caagtgagag
      421 tgttctgaag agccctttcg gtgcaagtac ttcgatcatc agtattgaga gaatgaacgt
      481 gaacgcagta aagaatgaaa aatcaaccca tgtccccacc agtgttccct gcgaagcaac
      541 gtgatcgaaa tactttataa caaaaggaac tttgtgcagt aggaaaagaa taatttgcag
      601 gagcactgtt ccagctgaaa caaagaacat agtaagaaag agctttacca atcggcgctg
      661 ctcacgggca aagagcgaaa caagaaacgc aaagagagca agaatggcaa tggttgaaaa
      721 cgaatcaaag ttgaatatgt ttccccagag cgacactggt acgtttggtg aaaaaagact
      781 cgataaaaat gagagcgctg ccactataaa caaccctaag agtgcgcggt gccgcggaaa
      841 ttgcattgtc ccctcaacaa attggctaat tatccaggtg gtaccagtga gcaaaacgcc
      901 gagcgagagc aatgtgtact ttacggctgc aaacgatcca atcgttgcag gtaaaaagag
      961 gagtggtaag agcgcacagg tacacaagag tagaacaaac gaaacattat aaaatcgaag
     1021 cgcaacaggg ttttgcatat agtggaatga tactttattt tctaccgctt gtgaactgga
     1081 atattacacc acgatattca caatcttttt ctgcggtaat gaaatcactt ttttcacttt
     1141 ttttccggcg aggtgcggag caactttttc aagaccgagt gctatttttt gtgcagcttc
     1201 aggggcgata gttacaggag cttcaaactg tccgcgaatt tttccattca cctgcaccat
     1261 aatcgttacc gtatcttcaa ttactttttt cttgtcccat actggccaca cagaaagatg
     1321 gatgctcttt ttttctccaa gctgctgcca gagctcttca gcaacatacg gtgcaaacgg
     1381 cgctaagagt cgtacgaata taccaaattg ctttttggta agtgctcctt caatactgtt
     1441 ggtaaaaatc atcattgccg acactgcggt gttgaagtta aagctttcaa tatcctctgt
     1501 tactttcttt attgttttat gcaatagctt ttcgttctgt tcgctcggtt ttacagcgaa
     1561 tggtgtgcca gccaatttcc atatacgctc aataaagcgg cgcgaaccga taatgccctc
     1621 agtcgaccac ggaagcgacg cctcaaacgg accgataaaa ctctcgaaca agcggagtgt
     1681 gtctgcgccg tatgttttta cgacatcatc aggattcacg acattccccc accgcttcga
     1741 catttttcga ttatcggcac ccaagatcat cccttggttt ttaagctgct ggaatggttc
     1801 ctcagtaggc acgagcccta ggtcgtacaa aaacttgtgc caaaagcggg catagagcag
     1861 gtgccctgta gcatgttcgg ttccgccaat gtatacatca acatttttcc aatacttgat
     1921 agcctgcttg gaagcaaaag ctttcttgtt cttcggatcc atatagcgga taaaatacca
     1981 agagctcgcc gcccagccag gcatagtgtt tgtctcatac cctttctgca cccactcttt
     2041 aatattcgca agcgggctct caccggtacc tgatggttta tatgatttta cgtttggcag
     2101 cttgagcgga agcttgtttt ctgcgagcgg cacaatcgtg ccgtcagact tgtgcaccag
     2161 tggaatcggt tccccccagt accgctgccg cgcaaacacc gcatcgcgca tcttatattt
     2221 tgtaacaatc ttcccgccaa ccactttagt aatttttact tttgcttctt cagaggtgag
     2281 accgctaaat tctcctgaat ttagcgttat tccatactca caaaaaggtt tctcctcagt
     2341 ttcagcaaca gttccatctt taaaaatgac atgtcgaata gatagattgt atttcttcgc
     2401 aaacgcaaaa tctcgctcat cgtgcgcggg caccgccatc actgcaccag tgccgtatga
     2461 agcgagtacg taatcagcaa tccatactgg aatttgctcc ttggttgccg ggttaattac
     2521 tgttacccct tctagtacta cgcctgtttt ttctttcgct tcagcagaac gcacttcgtc
     2581 tggtttattt gccacttcgt gtatatattt ttctaccatt tcccagttgc gaatatacgg
     2641 gcgtagtgtt tgtacaagcg catgctcggg tgcaagcacc aggtaggtgg caccaaacaa
     2701 ggtatcggcg cgcgtggtaa acacggttac cgtttcgcca ttcgagagaa tgaacggaat
     2761 ttcactcccc tcacttttac caatccagtt ccgctgaatt tcttttatcg ccagcgacca
     2821 gttgatagct tctaaacctt gcaaaaggcg ttcggcatac gcactgatgc gcaaaaacca
     2881 ctgccgcatt tttcgctttt ctacggggaa attgccccgc tctgaaagca gtgttccgtt
     2941 tggaccatcc accacctcat cgtttgcaag cacggtgcca agctccggac accaattcac
     3001 ttctgaatag ccctcgtacg caagccggta cttcatgagc actttttctt gctctggttt
     3061 tgaaaacgac ttccactctt tcgcagaaaa tattggcgtg tcagtatcgc agacagcagt
     3121 actattctta tttccttttt tctcaaactc ttttacgagt tctttagttg cacgggcttt
     3181 attcttttta gcatcgtagt aagcatcaaa cagtttcaaa aaaatccact gtgtccattt
     3241 gtaatagtcg ggacgatggg ttgccacctc gcggctccag tcgtacgaga gccccataat
     3301 tgaaagctgc tgcttgtagc gcctgatgag tgtttcagta aatggtcccg ggtgcttgcc
     3361 cgtttgtatg gcgtactgct cagccggaag cccgaatgaa tcaaacccca tcgggtggag
     3421 cacattgaag ccttgcatac gcttcaagcg tgcatagata tcgctggcaa tgtagccacg
     3481 cgggtgtccc acatggagcc cggtacccga cgggtacggg aacatatcaa gcacgtacat
     3541 tttctttttt cgactggtac tttcagcctt atagataccc tttctctccc actctttctg
     3601 ccacttcttt tcaagactct ggtggttgta gcgcttcata gtctaaatat tcttgcactg
     3661 taaggctaaa attgcaaaca ggacagaaac actttaccgc ttctcaggct ttgggaaggg
     3721 cggatagagc tctgggtcaa tctcgcgctg gtagcaggtg cgaccagcat catgatccca
     3781 attgttattt atatttgata tacctggata ccctactgaa gtttcagcac gatccatcat
     3841 cggcactggc atgtcacctc cctgcccctc aggcacccac ccttctggca tcggagcagc
     3901 aaaggtggca cagagttcga attttagatc agagaatttt cggtattcat aggcatttcc
     3961 tttctgaaat tctggatctt gcggcaccag gtagcttgag atcggcgttg cgatatcgac
     4021 aagtgtttca ggtactgttt ctttctgctg ccaaaaacta attacctgcc actggattga
     4081 ttgcaagtcg tcaatgcggc gctggtcgag cctaagcttg cgttgactgc ccggaccgcc
     4141 aataaccgta aagccatata tgatagcaac taaaaccaag acgctcgaac caattgcaaa
     4201 ccaccgtgct ttagaatcag tatgatttac cgagcgcttg agctcgcgaa tgtagtacca
     4261 accgacagca cttgctgcaa taagtgtcac ggcaactttt aagataaacc gcacggttat
     4321 ttcgcctgaa acaaagtaac ggaccagtgt taccaaatca gcgatgatca taacgctcgc
     4381 taaaaagagc actaggtata ttgcccagcg gcgcagtact tcatcccaat ggctcaagtg
     4441 ctgctttgcc gctcggagcc aaaagtgcat gagtgtcaaa aacactggaa agacaataat
     4501 aaggagcgcg agcgagttgc ggagtttttc aaaactgtag ctgtagtagc catagcggta
     4561 tgaatcggtg agtacgtcag gaaaggtatg attgaaggct tcaaacaaga gctgcaggag
     4621 cccagtaaca cttacgataa gcgctaccaa caccccaatt gaaagaaaga aaaataaagg
     4681 cgaaagtttt tttgtttctg tcatataccc tattctacac tgaaactaaa aaacggacac
     4741 aaaacaatga actattgata tatttgaatt agtattgtat agtgcaaaaa aatataccat
     4801 gagcatacga ccattgaaaa taacaaaatc tattacgaac cgagaacaag attcggtaga
     4861 aaaatattta agtgaaatcg gaaaagttca attaatagaa cccgaagaag aagcggttct
     4921 tgctcggaaa ataaaagaag gcgaccaagc tgccctagac aaactagtaa aagcaaatct
     4981 ccgttttgtg gtttcagttg caaaacagta tcaaaaccaa ggattatctt tgtcagactt
     5041 aatcaacgaa ggcaatctgg ggcttataaa agccgctcaa cggtttgatg aaacccgcgg
     5101 attcaaattt atttcgtatg gagtatggtg gatacgccag tctattttac aggcattagc
     5161 agagcaaagt cgtttggtgc gcctacccca aaacaaaatt gctctaacga ataaaattca
     5221 gaaagtgtat ttagagctac agcaagagtt cgaacgtgaa ccttcagccg aagagttggc
     5281 agaattattg gaaatagagg taggcagagt tgatgctgct agcattaatt ctcgctcctt
     5341 ttcactcgat ggaccactac cagaaggaga tggagatact tctttctacg aaatttatcc
     5401 ggatgatatc ctatcacccg atgccgaact cgaaaaagaa tctttacagc gcgacttaga
     5461 agaagcgttt aaacgactaa gtccaaaaca gcaagagatt ttaaaaaagg cacatggtat
     5521 tgggtacgat ttttcctgga gccatgaaag tattgcagac gaatacaacc tgacgaaaga
     5581 acgtgttcgt cagattatcg aaaaatctga agatcgattg gcaacctgta aaaatcttcg
     5641 acagtaccga ccgatcgaca tgaaaaagga tgtcaaaaaa aagaaaatca gaaaaaaggt
     5701 taaaatttag atggtagtaa aactcaacgt gtcattaata cgcacgttga gttttttatt
     5761 ttcaagcatt atgtgctaca acacatacat gaaaaagcct tcgacggtca gagaaaaaat
     5821 cgaacgccgc gctgaccgag ctacggaatg gattggttct actaaatcag tactcgtgca
     5881 cagtattgtg ttcgccgtag cctttttgct gccacttttt ggtgcagatt ttgaccgcgt
     5941 cctgcttatt ctcaccactg ccgtttcgct tgaagcaatc tacctagcga tttttattca
     6001 aatgacggta aataaaaaca ccgaagatat cgaggagatt caggaagacg tcgaagagat
     6061 tcaggtggat gtggacgaaa ttcaaaaaga tgtggatgag attcaggaag atattgaaga
     6121 tatcgaagaa gtacagggaa aagaagaaat agaagagctg caaaaacaga aaaaagatta
     6181 cgacttgtta agcgatatgc aaaagacgct taaaaagatg tcagaagaaa ttgaggacat
     6241 aaaaaagcgg acttagatct taaacgcagc gcataacaca catcaagttt tttatttttt
     6301 ggaaaagtga tagtattaca ttattcgtag agagaaacta gccactcttt ttctatttgt
     6361 ctttctccgg aagggtagac acgaattagg ctgccgcaag agttaggggg tcaagtagcg
     6421 acaagcgaat gaccttgcac atcaccctaa cagaaccctt gcggcttttt tatatcctaa
     6481 actctatagc actaccgtct tcgcaatttc ttaattcata gtcccaacga ccaccttggt
     6541 caagacaact atcaatttta atgaagatta tagccttata aacacctaag actgtggcaa
     6601 tgataaccaa tatgattaat aactttttca taattttatt catcagattt taaactcaat
     6661 cacatcccca tcttttacaa catactcctt cccttctatt cgcaccagcc ctttgctgcg
     6721 cgcttcagcg tacgagccag catcgagcaa atctttccaa tatactacct cggcacggat
     6781 gaatttttct ttaaagtcgg tatggatcgc agtgccggca ataggcgctg ttgagccttt
     6841 cttaatcgtc caggcacgag tttcgtcttc gccagtggta aagaaggttt caagcccgag
     6901 gagttcatag gcagctttaa ttaagtcgtc aaaccctttt tcaaacactg catcaattac
     6961 cacgtgcgct cccggaattt tttgcttcac tccctcaatt acattttctg caacctcaga
     7021 ggcattgagc gcatagagta tcggcttcat cgtgagcagc ccgagctgct ttacgagcgg
     7081 cacttctttt tcatcaagcg aaacagttgc tgctaatttc ccttcttcta gtattggaag
     7141 caattttgcg agcgctccgt cttctagtac cgcctgttta tcaccgcgct ttacttcttt
     7201 ttgaatgttc cccctccgtt ttgaaacggt ttccatgtca gcgagtacca gctccatatt
     7261 aataactgaa atatcatcga gcggatcaat cttgtttgct acgtggataa tgtccttatc
     7321 ttcaaacacc cgcaccattt ctaaaatcgc atccacttcg cggatattcg agagaaactt
     7381 gttacccaac ccctcgccct gcgcagcgcc ttttactaag ccagcaatgt caacaaactc
     7441 gatcgccgct ggaacagttt ttgcagattt tgaaaaatca gaaagttttt gcagccgctc
     7501 atcgggcacg gcaacaatgc ccaccgacgg gtcaatcgtg cagaaggggt aattttcagc
     7561 tggcacccct tttttagtga gcgcattaaa gagtgtcgat ttcccaacat tcggcagccc
     7621 gacaattcca atacttagtc ccatacaacc ttacttctcc tgcatcagct tctgcatttc
     7681 ggcttggtga tcgcgcatca cttccattgt tgctgccatc tggtttttac cctgcttcat
     7741 tttcatcttg atttcctcgc cgattttttt gaagaaatcg gggtttttgc taatcactgc
     7801 aataatccgc tcctgctcgg cttcgggcac ccctttgagc tggtttttga gcatcttttt
     7861 cattaacagg tttttcagtc caaacatgcg caaatgctac cacattggta gcataatgat
     7921 caataaccaa gcatcacatt ccaaacaata ccaaatgata taatgatgaa atgaaagaat
     7981 ttgatcttga aaaaagaaca tttctctttg ctcaagcaat ccgaactttt atcaaagagt
     8041 taccaaaaac tatcacttct tttgaagatg caaaacagtt agtacgatct tctggttcag
     8101 taggagcaaa ttatatagag gcaaatgaag cattgagcaa aaaagatttt cttatgcgta
     8161 taaaaatttg ccgcaaagaa gcaaaggaat caatttattg gttgcaactt attgaaatac
     8221 cagaacattt attagaaaag aagaaacagc ttattgcaga atgtacagag ttattaaaaa
     8281 ttttttctgc tatagtatca aaaagttctt agatttttaa tttaattatt tagagattgt
     8341 ttgattattg gaatttgatt attgattatt gcgtatgtcg aagacattag ctgaaataca
     8401 tgaatactgg cgcgagcact gcacctgcga attaaaaaag actgcaactc aagcagtatt
     8461 tggcgatggg aacccgcagg ctgaaatagt gtttattggt gaagcaccag gtaagaacga
     8521 agatttggaa gggagaccgt ttgtgggcgc tgcaggaaag tttttagccg aaatgcttga
     8581 gtcaatcaag atgcagcgat ccgacattta catcactaac attgttaagt atcgcccgcc
     8641 aaacaaccgc gacccgctgc cagaagaaat tgaagcctgt aaagaatggc tgtacgaaga
     8701 actgaacact attaaaccaa aactcatcgt gttcctcgga cggcactcga tgagccactt
     8761 ttttccgggc gagaaaattt caatcgtgca cggaaaattg cttataaaaa agattccagg
     8821 aattgaaaca aataatttcc tgccgctcta ccatcccgcc gccgcactct ataacggcgg
     8881 catgcgcgaa acactgctgc aggattttaa aaaaatcccc cttgcactaa aaaagataca
     8941 agggacagaa aaataactac ttcttcccta tctccaaagc gagaaacatt ggaaactcct
     9001 ttcgtgctct atcttctgcc tcttggcgaa tgccgggggc tgatttttta tgtgaaatcc
     9061 actcttccat cgcggtaatg gaaaagccgt gttttttaaa caccttcatg taccactgca
     9121 gactgcgatg gtaggtaact gtttttgctt cttttgttcc tttaaacgga ttctgtgaaa
     9181 tttcgatcgc cgcttctgaa agatatttat caacccggcg gtactgcaga ttcttttttg
     9241 tatcaaaccc ccaatctgaa aattgcggaa tccgaaatgc agggtggagc atcacgagca
     9301 ctactctccc ctcttttttc aacactcggg caatttctgc gaatgtctgg tcaatatttt
     9361 ttatgttttc aagcgccagc acaataagtg tggtgtcaaa cgaatatgct ttcaattttg
     9421 tatcgtgggc gggcgcggcg aagtagcgag cagtgccacc cttttcttta gcacaggtaa
     9481 tgagctcttc tgaaatatca atgccggtta ccgccgcgcc cgcctccgcc aaaagtcggg
     9541 aaaaataccc ttgtccgcag ccaacatcaa gcactgcttc tccctttttc aactcgagca
     9601 tccgcagcaa gtttggtgca attacttttg cttgatacga atcagcactt gtttctaagt
     9661 actcgtcgta ccacggcgct actgaactcc acgatgtact gttatttttc atgatgtttt
     9721 ggcggttctg gcggtaaaca ctcctcttca ttcttgcacc cgccggcaat gcctccatat
     9781 ttttttcctt cacgaagcac ataccataat cggcggaacg gtaaaatgtg cgcgtagtat
     9841 cgcacatcaa caagggtgcg cagcacccag ggcaaaaaac cgtacgcgcg gaaacgattc
     9901 atggcaagaa tcgcccacct gcttccaatg ggaataatgt acgccccttt gtgcggattg
     9961 tatggtgtga gcgtctttcc agtaattact tttttaattg cttgagcaac gtacttgcca
    10021 ttatgaattg ccgtttgtgc gagcccactg tacggagttt cagcgccatc acccacaata
    10081 aaaaaattgc tcagctcggg aacacggagg tactcatcaa caaccacccg cttccgtgca
    10141 ctaaatgcaa gagcggggat ttttgcaaat tgttcgttga tttttgtacc tgccgtccag
    10201 ataaccgttt ctgttttcat ctgcatatca cgcaggtgca cctcttcaat atctttttgc
    10261 attagtgttc ggttggtgaa gatgttaata ccaagttttc gcagtcgtgc ttcagccatg
    10321 cgagaaactg attcaggcaa gctcggcaag acacgagggt ttgattctat aagatcaatc
    10381 gtaacgagcg atgggtcaac gtgatgcttt tcagcaagcg tttgcaggta ctcttttaaa
    10441 tcgcccgcaa gctcaactcc cgacggtcca gcgccaacaa taacaatgtg gagcaatgag
    10501 acaagctctt ctttaacacc gttcccctgg gtaaagagcc ggtagaagtg gttttttaag
    10561 cggagcgctt cgtgtaccga tttaaacgag agcgaatgtt cagccagccc gggaatgctg
    10621 aagtagttgg tctggctgcc gattgcaagc accaggtaat catactcgta gtgagaaccg
    10681 ctctcgccaa tcacccgctt ttcttcagga ataaaagtaa cggcgcgatc gtgctctact
    10741 gtaatggcag tttggtcaaa aatcatccag agtgggatag aaacctcggt ggggagtgca
    10801 ccagttacta ctttatagag cgccggatag tactcgaagt aggttttgtc tgagagcagg
    10861 gttacttggt actggtcggc aacatctttg agcccgagtg ctgtttcaat gccaccgaac
    10921 ccgccaccaa caatcaaaat tcgtggctta ctacctgtgt tcatgggctc tagtatagca
    10981 gattggtagc cagaaatagg gctctagcgg tatactagag cggtacacgg tgctgccttg
    11041 acagccactg taaggtgcag cattattgct cgtctaagta acagatgttc gatgaatccc
    11101 cacacaccag gcaacccaga tttagagaag cttttcttcg agctcttcga agcctacaac
    11161 gacaccatat tccggttcgt ctactttcag ctccgcagcc gtgaacaggc actcgatatt
    11221 acccaggagg tcttcatgaa cacctggcgg tacctggcgc aaggcaagca gctcgaaaac
    11281 cccgaagcat tcctctaccg gagcgctcga aacgcggtga tcgattttta taagaagaaa
    11341 aaatcgctct cgctcgacgg cttaatggaa gatgggtttg agccaggggg cgacgatgag
    11401 cttaacctgc atctcaagca gggagactta aacctcattg ctgaagcggt gagaatgctc
    11461 gacccaaaag accagcaaat tttactgctc cgctataccg aagagttgcc gattgaagaa
    11521 attgccgaac agtttggcaa aagcacaaac gcaatgactg tccagattca ccgaatcatt
    11581 cagaaactca aaaaaatata cgaaaccacc tatggaagat aaccttaaac aattacatac
    11641 actaaagcgc attacgctta ctgccacgga gcagcatgca atgcgcagta ggcttgcgca
    11701 ctatgtacaa gaaacaccga ttgaaacaca atcgatgttt gaggtgtttg tttcgcacgg
    11761 cttgcgggtt gcgctttcat cgtttctctt ttttatactt attggcggga gtgtttcagc
    11821 gcttgccaac agctcgctcc cgggcgaccc gttctataat gtgaagctca atgtaaatga
    11881 aaaggtgcag gagtggctca ctaaaaatac ccctgagcaa aaagttgcct tccagcaaga
    11941 gcggattgaa aaccgcctga aggagataca attactcgcc gagactaaga cactcaccaa
    12001 agaaaagcgt gcagttgccg aagaggcact ctcgaaacac accacaaaat tctctgcgga
    12061 gcttggcgaa ctttctctaa aacagcctga agcagcgctc aaggcaacag caaagcttga
    12121 agaaacactg cgggtacaaa aagatgcact tacccttgct cctgaagcgg cagcaagcgg
    12181 gcaaaacgaa gcagtacttg aagtagtgga tagtgcactg gcatcggtag tgcaagaaga
    12241 gcagcgaatt atccaagccg aactttcaaa agtagaagat gaacttctca atgaagaccc
    12301 aatcgacgat atagacagcg ctcttgctcc cagcgatgaa gcaccggcgg atatagaaga
    12361 aaaacagtca tcatcagaac ataaaacaga tgaggtatcc gctaaaaatg aatcacctat
    12421 tccacctgtt actcctcatg agccttgaac cgctcaagga gtaaaaacag ggcgatagcg
    12481 atagcagcaa gcgcaccgag ggcaagaacg ccccaggtgc gtttgttgtt ttgtgttgtt
    12541 gacacggctc caagcgcatt cgctgcaagc ggctgctcag aagcagttac tgcgggttca
    12601 ggcaaagcga cactcttttg aaattgcaca ccagcaaccg agcgagtttt tggtacagaa
    12661 gctaccgtta tcgcctgtgg accagcaacg gcaatactgc tcgggtttgc taaagttggc
    12721 agttctgaaa aagaagcaac acccacgagc gctccagaaa cagggagcct attttttgct
    12781 ttcacaatac tgtaccgcgg aaattcatac tgatttcccg caaacaagag cttccactgg
    12841 tacagatcaa tattttcagt actggtattt tttataaaaa gtgcaccagc actatcacgc
    12901 tccagcgtaa gtaccggatt ggtaaccgag agtgaaaatt gccccaccag ttctgcgtcc
    12961 gaagtaattg cttcacgacg agtatacgca agggtaataa catactcgcc cggatactgg
    13021 taggtgtatg taaccggctc taggagctgc tgttcaataa ctcttccttc gcccatattc
    13081 catacaaaat gccctgcccg tgttactccg ctctcctcag taactgtcgc agtaatgcgc
    13141 accggcacac cggtaaatgc ttgctttgga tatataaacg aaatattcgg tttccatata
    13201 atctttggct cggtgctccc gccgccggaa atagtatttg tttttgaggt ttcagtttca
    13261 ggagtgtttt cgtcatcgac atgttcggtg tttgtgtttg ttcctacatc tactgttgct
    13321 gcttttggag tcggagcggc agtaatccat ccgcttccct ttttctgcat tgtttctttt
    13381 gttgctgcat cacccgccgg ccagcccgct tggaacggaa gttcgagcac tgtagtaccg
    13441 cttgcatctt ttaacaccag atgctcaccc gtattagcaa gccctgaacc gccaaaagtt
    13501 cccgcttcgt ctgaaatagc aggcagcgga tcaggcacgc ttggagttgt tcgttcaaca
    13561 accaagtatc cgcccgcagg aatagtttta gtgagtgaaa acagcagagc actgccgccg
    13621 tcttgataca gcttccatcc atctaaagaa acagaatcag ttccattatt atacagctcg
    13681 atccactcgc catactggct ctcagcagtt cccatccagg caatttcggt aacatcaacc
    13741 gcagcaaaag ccaccgccgg aaacgcggcg agtataatgc cgatactaag actattactc
    13801 aagcaccttc ttaagcacct catgtgatac ttctgataca tcttcttttt ctttactccc
    13861 cgcagcagcc ggcggcggct gtgcagcagt atgaacagga ggctgttgat cttcagcagg
    13921 ttttggtgtt tgtgttttag cgagcacagt gcggagcatc gctctactat cttcagtggc
    13981 agcacggtca agcactcgcg ctggctttgg tcggaggttt ggcaaaaagg ggttgtgatc
    14041 tgccggtgca gcaggagcag atgtttgttc ctgtttttct tgtgctgccg gttttgatgg
    14101 tggaggcgcc ggtggagaag ctgctggtcg cggtgaaggc tgcagtaccg gtgcacggtg
    14161 cgctgcttga aacgatggct gctccggttt cttttctaaa tcaagcgttg ccaagaatga
    14221 ctgcagggat tcttttggcg gttctttttt ttgcactgat tcgttttgta ctggcggctg
    14281 catcgtacca agtgctgcct taaacggctg atgctctttt ggctgctcgg gagttggcgg
    14341 cttttgccat gccggtgcgg gcggtttttg gaactgctgc actggaggtg tggatattgg
    14401 cggacgtggc tgctctcgca gcggaggacg acttacatac gattgttgcg gctggggacg
    14461 aaattgagat tgctgtggtg ctgcttgctg ctgatactcc tgccgcggcg ggcgctcacg
    14521 cacgggagct tcttctttta tataccaatc gccaattccc ttctctacct catcttttgg
    14581 tgctgcgtag acgctccgcg aatattcaat aacatcattt ctaaacgaaa tatccggaaa
    14641 cggaattggt aaaaaagttt ttgcagaaaa tggttttgag ccggtgccgt ctatcatgag
    14701 cgttaaataa atttgtccca tacctaaact caccatgtcg ttgggggtaa agtgctgtga
    14761 aaacgctttc tccaagagtt ctgcatcaag cggaccgacc cgaaaactca ccagtgttcc
    14821 cacgtttccg aacacggcat ctcgcaccga ttcttccatc tgtgctacgt actgatgggc
    14881 aataatcaaa ttgagcttgt atttgcgagc ctcggacaaa atgttcgcaa acgtttcatt
    14941 cgcaaatgac tggaattcgt cgacgtaaaa gtaaaagttt ggcagctcgg caagctttgt
    15001 tgggtgcacg tcagcacggc tcattgctgc caaataaatt ttggtaatga gcatactgcc
    15061 gagcaggctc gtgttttgct cgccgatgcg ccctttcgag aggttcatca ccacaatttt
    15121 gccttcgtcc attgcggtac gaaagttaaa gctcgatttt ggctgtccaa taatattacg
    15181 aatgagtggg tttgcagaaa actgcccaat cttgttttga atcgccgcac ccgcttcttg
    15241 catgtagcgg tcgccgtact ttgcaaattc atcaagccaa aatgccttca cccctgggtc
    15301 ggtcgcgttc gccactactt tgttgcggta gtctttgtcg gcaagcatgc ggtttacccc
    15361 caaaagggtg gcgcccggat actcgagcag cgccagtaaa atattattta aaatgtactc
    15421 catgcgggcg ctccaggcat ccacccaaat tttcttgaag gtgctcatca gtcccgaaac
    15481 cacaaagtgc cgcttgtccg cacccacgtc ttcaagcaca ttaaatgaaa cggggtagtt
    15541 ggtatcaaac ggcgcaaagt aaataacatc tttaatgcgg tgctgcggaa tgtagtcaag
    15601 aattttttca acaaagctgc cgtgcggatc gaggatgccg ataccgttgc cgttttttac
    15661 atcctgaatc gccatgtttt caagcagggt ggatttaccc acaccggtat ttccgatcac
    15721 atacatgtgc cgagggcgat ctttcgcttt tatgccaaag cgtgtttttt gattccgaaa
    15781 atcagtttcg gcaaagtagg ttacttgctc tgggtcaaat gccatatacg ctacagtata
    15841 tcatttgagt acagccgtgt gtgtagtgct ccattgatag agcaccaccg cagcagaaac
    15901 tgcagcattt aacgattcag tccgcgggtg catcggaatc cgcagggtaa catcacagag
    15961 ttcgagtgtt ttttggcgga ttccctcgcc ttcgttccca accaccagca ctgttggtgc
    16021 atcaaatgat tcagtaccca attcctttga tccctccatc gccagcccat acacccaaaa
    16081 acctttttct tttaatgcac gaagtgtggt atttacgtta ccaatccgca cgagcggaat
    16141 tcggaacgcc atacctgcag aagtttttat caccgcagct gttattggcg cttgattgtg
    16201 ttctggaatc agcacagctg tggcgccaaa ccctgctgcc gagcgaatga ttgcgcctac
    16261 attgtgcggg tcttggagct catcaagcag caccaggcag gtgtcagcag ttactgaaag
    16321 atgattgata aaggtatcga acggttgctg gagtttctcg gtatcaacga ctgcaataac
    16381 tccctggtgc gctgcatcag ctgccacctg ccgcttctct tcttctttga gtggcacata
    16441 gggaatattg tgtgtatcaa gcaatttccg aatatcacct ccaatagcct cagctaaaaa
    16501 tacctttttc actgcctgcg gcatatgagc gagcgcttct gtcaaggcgt gcttaccgta
    16561 catgtatagt ttttccgatt tcatgtactg aaaactatac cggtgaacgc ccgctggcgc
    16621 aacatgaata agtgctatac taaagaaaac tttttgtatg aaaattcaaa ccttatgtgc
    16681 tggtatgata tggattccat tctatatttt cacctttaca gtagccggtt tttttgcgct
    16741 actcgcagcc ccttttgatc acattaaaaa aacataattt taaaaagccg cccttttaaa
    16801 aataaggacg gcttttattt taactattca ttacatagga tgggacaccc tatgtgtcgt
    16861 attgcttttg cttttaactc tccgaccgcg aatggggtaa ccccgagctg tttagcaatt
    16921 tctttttccc ccactccggt atagaacgat tccaagagtg cca
//
LOCUS       DATIBQ010000049         3178 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U16294
            k141_1340445, whole genome shotgun sequence.
ACCESSION   DATIBQ010000049 DATIBQ010000000
VERSION     DATIBQ010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935789
            Sequence Read Archive: SRR25158331
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:58:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 637
            CDSs (total)                      :: 603
            Genes (coding)                    :: 601
            CDSs (with protein)               :: 601
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3178
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1340445"
                     /isolate="SMAG_U16294"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..760)
                     /locus_tag="VLB02_01495"
     CDS             complement(<1..760)
                     /locus_tag="VLB02_01495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56739.1"
                     /translation="MKSLAKQYGCFFLLAFTLVFADTASSFAQSGAGGLGQIPFATIR
                     FGLECRDGIDNNGNGLVDTNDTNCVTKNDASELLPGTQADEIVIKDIACDGREDFLGI
                     NMVAPPFPGVIAKIAWAPVQLAWKTLLCPAKVDRNCDGRSDLTGIAVFMEQHHPDQCP
                     NGYTTKNVDRNCDGRDDRYPNIDVRRTDYWNQICPDPGKYTDANCDGYDDKLGIDMLD
                     PKVDPNGPSVWYTLCPAKVDHNCDGRADISGVKYW"
     gene            complement(744..1298)
                     /locus_tag="VLB02_01500"
     CDS             complement(744..1298)
                     /locus_tag="VLB02_01500"
                     /inference="COORDINATES: protein motif:HMM:NF039493.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HSE56740.1"
                     /translation="MKGKLFTLLVSLFFLLVLLPPIAAQAQYVDDNCDGQDDVKAVTI
                     PPDKSPCNFSGPQQQTGGPQQQTGDPQQQTGAPQQQTGGSLQKTGKETQINIKIKNPL
                     GETDTIPEAIKKIMDVVLQLVFPFIVLLFIYAGFLFVTARGNKTKLEQAKTMFWYTLI
                     GTLLILGAWTIATAIVNTVNEITG"
     gene            complement(1316..1765)
                     /locus_tag="VLB02_01505"
     CDS             complement(1316..1765)
                     /locus_tag="VLB02_01505"
                     /inference="COORDINATES: protein motif:HMM:NF039493.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56741.1"
                     /translation="MYYKKNLLYSCFALLFFLQTFFSPVGVFAQASTDSAAYNPGVYT
                     DTGSDTPSGTLIPCTNECDFNDIMQLINNLINFSIEFLVLPIFIAILIYAGFLYLTAN
                     GKPGVHAKVRKMLLNALLGLLIVLCSWLIVKVLITTLGYKEGLKFLE"
     gene            complement(1767..3065)
                     /locus_tag="VLB02_01510"
     CDS             complement(1767..3065)
                     /locus_tag="VLB02_01510"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HSE56742.1"
                     /translation="MSNFQIALIALFIGIFGFAVLVFSGIIKIGRGSTQTASGDVLVW
                     GTYSETAMTAYLSDFSRRDKEYNISYVQIAPENFDQAFIEALASGVGPDIILVGNDSF
                     LRHQDKLFIIPYTSYPERTFRDTYLDGSYLYLTRAGIIGVPVTVDPLVLYYNKNLLAR
                     DGIITPPSTWEDLARMSPLIVGKSKQGGLETTTIALGEYANIPHFKHILSALFLQAGN
                     GIVAEDRLSNGYASVLQNTAPNGGTAADALRFYLSFANPVNELYTWNRSLPSARDAFI
                     GGTSAFYIGSASELFDIQSQNPNLNFDVALLPQPETNEQAVTYGTFTAASVVKSTKNF
                     SAAYAALLEFGSDTFATFMSKTLSVPPAHRALLLDRPQSPYLNVFFSTAAVTFSWPDP
                     DIAKTDQAFRDMIRNVSSGRTTPEEAVSEASKALQSAIRR"
BASE COUNT          843 a          737 c          754 g          844 t
ORIGIN      
        1 tccagtactt aacccctgaa atatctgccc gaccatcaca gttatgatca acctttgcag
       61 ggcagagcgt gtaccagaca cttggaccat tcgggtcaac ctttgggtcg agcatgtcta
      121 tgccgagctt gtcgtcatag ccatcacagt ttgcatcggt gtattttccg gggtctggac
      181 atatctggtt ccagtagtcg gtacggcgaa catcaatgtt cgggtagcga tcatcacgcc
      241 catcacagtt tctgtctaca ttttttgtgg tgtagccatt ggggcactgg tcggggtggt
      301 gctgctccat aaacactgca ataccggtta agtcagatct gccatcgcag tttctgtcta
      361 cttttgccgg acacaggagt gttttccagg caagctgcac tggtgcccag gcaatttttg
      421 caataacacc cggaaacggc ggcgctacca tgttaatacc gagaaagtct tcacgaccat
      481 cacaggcaat gtctttaatc acaatctcat ctgcctgcgt gcccgggagc agctcgcttg
      541 catcattctt tgttacgcag tttgtatcgt tagtatcaac taatccgttt ccgttattat
      601 caatgccgtc tcgacactcc aacccaaacc gaatggttgc aaaagggatt tgtcctaacc
      661 cccctgcccc cgactgtgca aaagaacttg ccgtatcggc aaatacaagc gtaaacgcca
      721 gcagaaaaaa gcacccatac tgtttagcca gtgatttcat taacggtatt tacaattgcg
      781 gtggcaatcg tccatgcccc taaaataagg agtgttccaa taagcgtgta ccagaacatg
      841 gtctttgctt gttcgagctt ggttttattc ccccgcgcgg ttacaaatag aaagccggca
      901 taaataaaca gaagcacaat aaacggaaat acgagctgga gcacgacgtc cattattttt
      961 tttattgctt cagggatggt atcagtctcg ccgagcgggt ttttgatttt gatgtttatc
     1021 tgggtttctt ttcctgtttt ttgcagtgaa ccgcccgttt gttgttgcgg tgcaccagtt
     1081 tgttgttgag gatcgcctgt ctgttgctgg ggaccaccgg tttgttgctg tggtccgcta
     1141 aaattacacg gtgatttatc aggtggtatc gttaccgctt ttacatcatc ttgcccatcg
     1201 cagttgtcat caacgtactg cgcctgagca gcaatcggag ggagtagtac taggagaaaa
     1261 aaaagagaaa ctaacaacgt gaagagtttt cctttcatat atcgtcaagt ataccttatt
     1321 ccaaaaactt aagcccctct ttatacccaa gcgtagtgat aagtactttt acaatcagcc
     1381 acgagcacag cacaatcaaa agcccgagca atgcatttaa gagcattttt cgtacctttg
     1441 cgtgcacccc tggctttcca ttggcggtca gatacaaaaa tccagcatag atgaggatag
     1501 caataaatat tggtagtact aaaaattcga tcgagaaatt gataaggtta ttgataagct
     1561 gcatgatatc gttaaaatca cattcgttgg tgcacggtat cagcgttcca gaaggtgtat
     1621 cggaacctgt atcagtataa acacctgggt tgtacgctgc actatcagta ctcgcctgtg
     1681 caaacacacc caccggagag aagaaggttt gtagaaaaaa aagaagcgca aaacatgagt
     1741 agagaagatt ttttttgtaa tacatattag cggcgaattg cactttgcag tgccttactt
     1801 gcttcagaga cggcttcttc gggcgtggta cgacctgagg atacgtttct aatcatatca
     1861 cggaaggctt ggtcagtttt tgcaatgtca gggtcaggcc atgaaaatgt tactgcagca
     1921 gtggagaaaa atacatttaa gtacggactc tgcggtctat ccaaaagtag tgctcggtgc
     1981 gccggcggta ctgaaagtgt tttagacata aatgttgcaa atgtatcaga gccaaactcg
     2041 aggagcgctg catacgcagc cgaaaagttt ttagtcgact tcaccaccga agcagcggtg
     2101 aaagtgccgt acgttactgc ctgttcattt gtttcaggct gcggcaaaag tgcaacatca
     2161 aaattaagat tcggattctg ggactgaatg tcgaatagct cgctcgccga gccgatatag
     2221 aaggcactgg ttccgccaat aaacgcatca cgggcacttg gtagtgaacg gttccaggtg
     2281 tagagctcgt tgactgggtt tgcaaacgac aaataaaacc gaagtgcgtc ggcagcagtt
     2341 cctccatttg gcgcagtatt ttgcagcacc gaggcgtagc cgtttgagag tctgtcttcc
     2401 gcaacaattc cattccccgc ctgcaaaaag agtgccgaga gaatatgttt gaagtgagga
     2461 atgtttgcat attcaccaag tgcaatggtg gtcgtttcga gtcccccctg cttgcttttt
     2521 ccaacaatca gcggactcat acgtgctaag tcctcccacg ttgatggcgg cgtaataatg
     2581 ccatcgcgcg ccaaaaggtt tttgttatag tagagcacga gcgggtcaac ggtaaccggc
     2641 acgccgataa tccccgctct ggtaaggtag agataactgc cgtcgaggta ggtatcgcgg
     2701 aaggtgcgct cggggtaact ggtgtacgga ataataaata gtttatcttg gtggcgcaaa
     2761 aagctgtcat tgcccactaa aataatatcc ggaccaaccc ccgaagcgag cgcttcaata
     2821 aatgcctgat caaaattctc gggcgctatc tgtacgtagc ttatattgta ctctttgtct
     2881 cggcgcgaaa agtcgctcaa gtacgctgtc atcgccgttt cagagtacgt tccccacacg
     2941 agcacatcac cgcttgctgt ctgcgtgctc cctcttccaa ttttaatgat tcctgaaaac
     3001 accagcaccg caaaaccaaa gatgccgata aatagagcga tgagtgcaat ttgaaagtta
     3061 gacatgctag ctatagtgta aggcagaaac ttcaaagttg aaagtagtga acctagtatt
     3121 ttttggagac tcccgcaggc gggtagccga gggcgagcaa aaaattcacg agaatttt
//
LOCUS       DATIBQ010000050         3332 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U16294
            k141_1675391, whole genome shotgun sequence.
ACCESSION   DATIBQ010000050 DATIBQ010000000
VERSION     DATIBQ010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935789
            Sequence Read Archive: SRR25158331
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3332)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3332)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:58:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 637
            CDSs (total)                      :: 603
            Genes (coding)                    :: 601
            CDSs (with protein)               :: 601
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3332
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1675391"
                     /isolate="SMAG_U16294"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..382)
                     /gene="rplD"
                     /locus_tag="VLB02_01515"
     CDS             complement(<1..382)
                     /gene="rplD"
                     /locus_tag="VLB02_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HSE56743.1"
                     /translation="METKTTTKKSATEVPALEAAVYSQTGAKAGTIALPAAVFGVKWN
                     ASLVHQVAISMMANARAGTAHTKDRSEVSGGGKKPWKQKGTGRARHGSSRSPIWVGGG
                     VTFGPRSDKDYSKKINKKMRTKALF"
     gene            complement(385..990)
                     /gene="rplC"
                     /locus_tag="VLB02_01520"
     CDS             complement(385..990)
                     /gene="rplC"
                     /locus_tag="VLB02_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005239628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HSE56744.1"
                     /translation="MKFIVGTKLNMTQLFDDNGTVIPVTIVKAGPVTVTNLKTAEKDG
                     YSAVQVGFGEQKESRISKATLGSLGGKPFRHVVEFRSDDAPVVAAGDIVDVSAFVPGD
                     VLTVTGTSKAKGFQGVVKRHGFHGGPRTHGQKHSEREAGSIGGGLRTRVPKGMRMAGR
                     TGGDTVTVKNLSLVAVDAEKGELYISGAVPGRRGTLLKIVS"
     gene            complement(1181..1384)
                     /locus_tag="VLB02_01525"
     CDS             complement(1181..1384)
                     /locus_tag="VLB02_01525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HSE56745.1"
                     /translation="MKSGFFIMKLWQTGNVGSCNIAKIKANPKKGYASDEEAKTAMKN
                     LHKNGDWELTQSGYSFTVTELFW"
     gene            complement(1529..1999)
                     /locus_tag="VLB02_01530"
     CDS             complement(1529..1999)
                     /locus_tag="VLB02_01530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56746.1"
                     /translation="MIADTITKFWQVYDEYTALHDSSLKFLLLSFLADQDEQVGNLTR
                     PEKGWNHILAWEVLKQHTFFLSRENIQFEVFEGEIIVVQRDWDRNVFCTRIPHQEFVR
                     GSILSMKEREGRIFLPLYEAAVVRAKTILFSQKFLRQTLEFLSKEEEEVWARYT"
     gene            complement(2191..2544)
                     /gene="rpsJ"
                     /locus_tag="VLB02_01535"
     CDS             complement(2191..2544)
                     /gene="rpsJ"
                     /locus_tag="VLB02_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HSE56747.1"
                     /translation="MTATKTTKKAPAKTEAQSILRIRVRAYENKILDASVKQIVDTAT
                     RYDAVVRGPIPLPTEIKKYTVNRSSFIYKNSREQFEMRVHKRLIDIENPSPKVIEALT
                     NLSMPSGVDIDVKML"
     gene            complement(2574..>3332)
                     /locus_tag="VLB02_01540"
     CDS             complement(2574..>3332)
                     /locus_tag="VLB02_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008985219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HSE56748.1"
                     /translation="ELIDLVEEEIRELLAKQGFDKDCPIIRGSGLKGLEATTLEDPWA
                     QKIMELVTALDTYIPLPERELDKPFLMPIEDIFTIEGRGTVVTGRIERGIVKVGEEVE
                     VVGIKDTVKTTVTGIEMFNKSLDQGQAGDNAGILLRGLKKEDLTRGQVLAKTGSIKPH
                     TDFEAEVYILKKEEGGRHTPFINGYKPQFYIGTTDVTGEVTLPEGTEMVMPGDTITFK
                     VKLMAPVALEAQRRFAIREGGKTVGAGVVTKIVA"
BASE COUNT          827 a          800 c          676 g         1029 t
ORIGIN      
        1 taaaaagggc ttttgtacgc atcttcttgt taattttttt tgagtaatcc ttatcgctgc
       61 gcggaccaaa cgtaacacct ccacctaccc aaattgggct ccgtgatgag ccgtgccgtg
      121 cgcgacccgt ccccttttgc ttccatggct ttttgccgcc acccgatact tcgctgcggt
      181 ctttggtgtg tgcagtacca gcccgagcat ttgccatcat tgaaatggca acctggtgca
      241 ccaagcttgc gttccacttt accccaaaca ctgctgcagg taatgcaata gtaccggctt
      301 ttgcacctgt ttggctgtac actgctgctt caagtgctgg tacttctgtg gctgatttct
      361 ttgttgtcgt tttggtttcc ataactaact cacaatttta agaagcgttc cgcggcgacc
      421 aggcacagct ccactgatgt agagctcacc tttttccgca tccaccgcta caagcgagag
      481 attctttact gttactgtat cgcctccggt gcgtcctgcc atgcgcatac cttttggtac
      541 gcgagtacgc aagccgccgc cgatagaacc cgcttcgcgt tcagagtgct tctgaccgtg
      601 ggtacgtggt ccaccgtgga atccatgacg cttcacgaca ccctgaaagc cctttgcttt
      661 tgaggtaccc gttaccgtca gtacgtctcc aggaacaaat gcacttacat caacaatgtc
      721 tcctgcggca actactggag cgtcgtcaga gcggaattca accacatgac gaaacggctt
      781 tccccccagc gaaccaagag ttgcctttga aatccgagat tccttttgct cgccaaaacc
      841 aacctgcaca gcgctgtagc catctttctc agccgttttg aggtttgtta ccgttactgg
      901 accagctttt acgatagtaa ccggaataac tgttccatta tcatcgaaca gctgggtcat
      961 attaagtttt gttcctacga taaatttcat gaatgttcgt gcccatctgc tttgcagcta
     1021 gatatgtggg tattggacca tttcctccta aaaaataggt attgatttgg tagccaggat
     1081 tatacacggc ttttattttt gtgcaagcat acagtttaag aaaaaatctc tatccaatat
     1141 caagaggtag agattttaaa taccattcaa aatatgaagg tcaccaaaag agttccgtaa
     1201 cagtaaaact atatccactt tgcgttaatt cccagtcacc gtttttatgt aaattcttca
     1261 ttgcagtttt tgcttcctca tcacttgcat agcctttttt cggattagcc ttaatttttg
     1321 caatattaca acttcccaca tttcccgttt gccagagttt cattatgaaa aatcctgatt
     1381 tcatgttttg tatgtattag gttttaaaaa gtttaatcag atactacctt gacttgctag
     1441 tttgtcaatt tttacactaa aaagatcatg ctaattagca ttatctcgaa tccagtaata
     1501 aagttaagaa ttcaaaaatt ctaattaatt aggtatagcg tgcccatacc tcctcttctt
     1561 cctttgaaag aaactcgagt gtttgtctca gaaatttctg agaaaacagg atagtctttg
     1621 cacgaacaac agctgcttcg tagagcggta aaaatattct cccttcacgc tccttcatag
     1681 aaagaataga gccacgtaca aattcctggt gcgggatgcg agtacaaaaa acattacgat
     1741 cccaatcacg ctgtacaaca ataatttctc cttcaaaaac ctcaaattgg atattttccc
     1801 gagagagaaa aaacgtgtgc tgttttagta cctcccaggc taaaatatga ttccatcctt
     1861 tttccggtcg cgttaggttg cctacctgtt catcctggtc agcaagaaat gagagtagta
     1921 ggaatttaag agaactgtca tgtaaagcag tatattcatc atatacctgc caaaatttgg
     1981 taatggtatc tgcaatcata ttgtttattt ttctctactc tattcatttt ttcttcaaag
     2041 tcaaagtgtt tacttatctt cgagattttc taattaaaag ctaaaaacca ccttttgaaa
     2101 ggtggttttt agttgagaaa gttacatccc ctcgcctgaa gcagactcag gactaaagga
     2161 aattatagtc gccagaggct tcctaatttc ctaaagcatc ttcacatcga tatcaacgcc
     2221 gctgggcatt gagagattgg tgagtgcctc gatcactttt ggtgatgggt tttcgatgtc
     2281 gatgagacgc ttgtgcaccc gcatctcgaa ctgctcgcgc gagtttttgt agatgaacga
     2341 cgagcggttt accgtgtact ttttgatctc ggtcgggagc gggatgggac cgcgcactac
     2401 cgcatcgtag cgggtagccg tatccacaat ctgcttcact gacgcatcaa gaatcttgtt
     2461 ctcgtaggcg cgaacccgga tccggagaat gctctgtgct tctgtttttg caggagcttt
     2521 ttttgttgtt ttcgttgcag tcatattttt ataaagagca gtgtattact gggttacgca
     2581 acaatcttcg ttaccacacc agcaccaact gtcttgcctc cttcgcggat agcgaagcgg
     2641 cgctgtgctt caagcgcaac gggcgccata agctttactt tgaaggtaat cgtgtctccc
     2701 ggcatcacca tttcagttcc ttctggaagg gttacttcac cggtaacatc agtggtacca
     2761 atgtagaact gtggtttgta tccattgata aacggtgtgt ggcgaccacc ttcttccttc
     2821 ttcaggatgt acacctctgc ttcaaagtcg gtgtgcggct taatactgcc tgtttttgcg
     2881 agtacttgtc cacgagtaag atcttctttt ttaagaccgc ggagcaaaat tccagcgttg
     2941 tctcctgcct gcccttggtc gagtgattta ttgaacattt caataccggt tacggttgtt
     3001 tttacggtgt ctttaatacc caccacttca acttcttcac ccaccttcac aataccgcgt
     3061 tcgatacgac cagttaccac tgtaccgcga ccttcaatcg taaaaatgtc ttcaatcggc
     3121 atgaggaatg gcttgtcgag ttcgcgttct gggagtggaa tataggtatc aagtgcagta
     3181 acaagttcca taatcttctg tgcccatgga tcttccagtg ttgttgcctc aagacctttc
     3241 aatcctgatc cacggataat aggacagtct ttgtcgaagc cttgctttgc gagaagttca
     3301 cggatctctt cttcaacaag atcaatgagc tc
//
LOCUS       DATIBQ010000051         4549 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U16294
            k141_1542203, whole genome shotgun sequence.
ACCESSION   DATIBQ010000051 DATIBQ010000000
VERSION     DATIBQ010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935789
            Sequence Read Archive: SRR25158331
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 4549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:58:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 637
            CDSs (total)                      :: 603
            Genes (coding)                    :: 601
            CDSs (with protein)               :: 601
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4549
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1542203"
                     /isolate="SMAG_U16294"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..417
                     /locus_tag="VLB02_01545"
     CDS             <1..417
                     /locus_tag="VLB02_01545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56749.1"
                     /translation="SVVPSAFELLTDLDAEAVLVFFTATPHPKDQKMKLAFEITKGIF
                     GEKAAAQAQTAFVHTFQKGEIPEQVKKAPIDNQSLDALLLAAGIVASITELRRLTDAG
                     AVVHLETEEKLATADIKKVPTPGTYRIGKNRFIKLV"
     gene            complement(425..568)
                     /locus_tag="VLB02_01550"
     CDS             complement(425..568)
                     /locus_tag="VLB02_01550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56750.1"
                     /translation="MDFEKAIGYVFNLIVFIVLSIIFVPAFFIVTYAQEYWSKKMADL
                     FKL"
     gene            644..1549
                     /locus_tag="VLB02_01555"
     CDS             644..1549
                     /locus_tag="VLB02_01555"
                     /inference="COORDINATES: protein motif:HMM:TIGR01777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HSE56751.1"
                     /translation="MTIVVTGGSGFIGSALTAALLEKGHTVVVIDQRAPALTHQNLYF
                     IQCNLEADLLPFNVLERTDAIIHLAGKPIFEKWTEKVKQAIKQGRVEATRHIIQSLER
                     TTTRPSIFISASAVGYYGEAGDTELDEKSPKGNTFLSDVVQVWEAEAAKAEQFGCRVV
                     IVRTAPVIGTGGFLAPLVRLARFWLVFKLGRRDFWQPWIHLEDIVRVYLFALETSTLQ
                     GVVNAAAPTPVRHSELMKHFAKAVKRRLLGALPLRLVKFLYGDFATEITTSQKVYPRR
                     LLDKGFTFEYTNVEGALRSITHEKK"
     gene            1536..2393
                     /locus_tag="VLB02_01560"
     CDS             1536..2393
                     /locus_tag="VLB02_01560"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HSE56752.1"
                     /translation="MKRSDVTTLGWDEYELLDSGEGRKLERFGDIVLDRPDTQALWKK
                     EHSVLWERAQASFSWADKGERWFLQTEIPQTWTLPYTELQFILSLKQFKHVGLFPEHE
                     PQWQEIAALCKAHPGLRVLNLFGYTGAASVVAAAAGATVTHVDASKQTIEIVKENLAA
                     SALPSDSIRLVCEDALRYAKRLVARGEQFEVIIMDPPAFGRGPKGEVWKIEEGLADVV
                     ALVPQLLSERAKLVLLNGYTAGYSARTFGELLHDVLPSGTVTFGDIGIAQKNSDRVLT
                     TGMYGKWEK"
     gene            2443..2862
                     /locus_tag="VLB02_01565"
     CDS             2443..2862
                     /locus_tag="VLB02_01565"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016279 - protein-lysine
                     N-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein"
                     /protein_id="HSE56753.1"
                     /translation="MKKKELVPLTSQLKVKRGINGLGLFTTKPIKKGTFIIEYVGEML
                     TADQANERGGQYLFEINSKWTVDGSSRKNLARYINHSCRPNCETDTNERTKRIFVTAI
                     KNIKEGEELFYDYGKEFWNDYIKPKGCRCEKCQARKQ"
     gene            3051..3440
                     /gene="msrB"
                     /locus_tag="VLB02_01570"
     CDS             3051..3440
                     /gene="msrB"
                     /locus_tag="VLB02_01570"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014033713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HSE56754.1"
                     /translation="MDEKEKEEKLKQLTPEELHVTQEKGTETPFQNKYWNNHQQGMYA
                     CKVCGAQLFASDSKFDSGTGWPSYDKALPGAVEYVEDTSHGMHRVEVVCKNCGAHLGH
                     VFDDGPTDTTSKRFCINSCSLDFKKEK"
     gene            complement(3498..3710)
                     /locus_tag="VLB02_01575"
     CDS             complement(3498..3710)
                     /locus_tag="VLB02_01575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56755.1"
                     /translation="MGNDYSALVGEEDVNAPTKEPIDLLEADGETDIVAPLVDETDEA
                     EEEEADDVFYMKQILEDQYPDGDIPS"
     gene            complement(3769..4278)
                     /locus_tag="VLB02_01580"
     CDS             complement(3769..4278)
                     /locus_tag="VLB02_01580"
                     /inference="COORDINATES: protein motif:HMM:NF014166.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HSE56756.1"
                     /translation="MQSKKIIAVDPGYDRCGIAVFICSPKPELLFSTCITTSKGDSHS
                     RRLQEIYRGIQTAITMHAPEVLAVETLFFSLNKRTAIKVAEARGVILLAAEEAGLAVL
                     EVSPQLVKLSATGRGNSDKQSVQKMVSTILKKDIATLLDDEIDAMAIGLAAAEELRVQ
                     KLFHKGIDN"
     gene            complement(4268..>4549)
                     /locus_tag="VLB02_01585"
     CDS             complement(4268..>4549)
                     /locus_tag="VLB02_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013012960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HSE56757.1"
                     /translation="TYEGYGPGGVGIVVQALTANRNKASAEIKHAFTKHGGSLGAIGS
                     VTWGFTKEAGTWTPTITIPLSDEDAEKLGELVESLEESDEVNEVYTNAE"
BASE COUNT         1183 a         1024 c         1150 g         1192 t
ORIGIN      
        1 agtgtagtgc cgagtgcgtt cgaactgctt actgaccttg atgccgaagc agtgttggta
       61 ttttttactg ctaccccgca cccaaaagat caaaaaatga agctcgcgtt tgagattacc
      121 aaaggaattt ttggagaaaa agctgcagcg caggcgcaga cagcatttgt gcataccttc
      181 caaaagggtg aaatccctga acaggtgaaa aaagcaccaa tagacaatca atcacttgat
      241 gcgctactgc tcgcagcggg aatagttgcc tcaatcaccg aactccgtcg gctcaccgac
      301 gcaggcgcgg ttgttcatct tgaaaccgaa gaaaaactgg caactgccga cataaaaaaa
      361 gtacccacgc cgggcaccta tcgcattgga aagaacaggt ttataaaatt agtataaaaa
      421 ttacctacag tttaaataaa tcagccattt tctttgacca gtactcctgg gcgtaggtaa
      481 caataaagaa cgccggcacg aagataatgc tcaacacaat aaacactatg aggttgaaga
      541 cgtacccaat cgccttttcg aaatccacct tttgatacta gcgtttttct cctatttggc
      601 aaattcgctt gaaaataaag ggtttttgtg agagaattgc cccatgacca tcgtcgttac
      661 gggcggatca gggtttatcg gaagcgcact cactgctgct ttattggaaa aagggcatac
      721 tgttgtagtg atcgatcagc gcgcgcccgc actcacccac cagaacctct actttattca
      781 gtgtaacctc gaggcagacc tgctaccttt taatgtgctc gaacgcaccg atgccatcat
      841 tcaccttgcc ggcaaaccta tttttgaaaa atggactgaa aaagtgaagc aagcaattaa
      901 acagggtcgc gttgaagcaa caaggcatat tattcaaagt ttggagcgca ccactactcg
      961 accgtcaatt tttatcagtg cttcggcggt ggggtactat ggtgaagcgg gagataccga
     1021 gcttgatgaa aaatccccca aagggaatac ttttttaagc gatgtcgtgc aggtgtggga
     1081 agctgaagct gcaaaagctg agcagttcgg ctgtcgggta gtaattgtgc gtactgcgcc
     1141 ggttattggt acgggcggct ttttagcacc gctggtgcgg cttgcccgtt tttggttggt
     1201 gttcaaactt ggtcgtcgag atttttggca gccctggatt catcttgaag acattgttcg
     1261 ggtgtacctc ttcgcacttg aaacctcaac actgcaggga gtggtaaatg cagcagctcc
     1321 aactccggtg cgccacagcg aactgatgaa gcattttgct aaagcagtga aacgtcggct
     1381 cttgggggcg ctgccgctcc gtttggtaaa atttttgtac ggagattttg ctaccgagat
     1441 aactacgagt caaaaagtgt atccgcgccg gctcttagat aaagggttta cctttgagta
     1501 caccaacgtt gaaggcgcgc ttcgctcgat cacgcatgaa aagaagtgat gtaacaactc
     1561 tgggctggga tgagtatgaa ctcttagatt cgggcgaggg aagaaagctt gaacgatttg
     1621 gcgacatagt acttgatcgt cctgacacac aagcattgtg gaagaaagaa cattcagtac
     1681 tttgggaacg tgcccaggca tcgttttcgt gggcagacaa gggcgaacgg tggtttttgc
     1741 aaactgaaat tcctcaaacc tggacactcc cgtatacaga attgcagttc atactctcgc
     1801 ttaagcagtt caaacatgtg gggctctttc ctgaacacga gccgcagtgg caagaaattg
     1861 cagcactgtg caaagcacac ccagggcttc gagtattaaa cttgtttggc tacaccggag
     1921 cggcgagtgt ggttgcagct gcagcggggg caacagtaac ccatgttgat gcttcaaagc
     1981 agacgattga aatagtaaaa gagaatcttg cagcgagcgc gctaccgagc gacagtattc
     2041 gtcttgtctg tgaggatgcg ctccgctacg caaagcgctt agttgcccgc ggcgagcagt
     2101 ttgaagttat tattatggat ccgccggcat ttggccgcgg tccaaaagga gaagtgtgga
     2161 agattgaaga ggggctcgct gatgtggttg cgctcgtgcc gcagttattg agcgagaggg
     2221 caaaacttgt tctgttaaac ggctatactg ccggctactc ggcccgcacc tttggtgagc
     2281 ttctgcatga tgtacttcca agtggaacag ttacttttgg cgacatcggc attgcacaaa
     2341 aaaattccga tcgtgttctt acgaccggaa tgtatggaaa gtgggaaaag tagttctagt
     2401 gctatgttat aatcttcgga ttatttttaa gtttctgaaa gaatgaaaaa gaaagaatta
     2461 gtgccgctga caagccagct gaaagtaaaa cggggaataa atgggttagg gttgtttacc
     2521 acaaaaccta tcaagaaagg aacatttatt attgaatatg tgggcgagat gctcaccgct
     2581 gaccaagcaa acgagcgcgg cgggcagtac ctgtttgaaa taaacagtaa gtggacggtc
     2641 gatggcagca gccgcaaaaa tctggcgcgc tacattaacc actcctgccg gccaaactgc
     2701 gagaccgaca cgaacgagcg cacgaagcgg atttttgtaa cggcgataaa aaatataaaa
     2761 gaaggcgagg agctttttta tgattacggc aaagagtttt ggaacgacta catcaagcca
     2821 aaagggtgcc ggtgtgagaa gtgccaggca cgtaagcaat aatcctcact ggtgcatctc
     2881 tcgaaccact caaacgtccc cagccggacg ttttcgttcg tcctcgggct cgctctccct
     2941 cagaagcctc gggaaccatg cccgctcgcc ctgcggatgt tctcgatgtg caccagctcg
     3001 tcttatctga gggctttacc cacacacctt tgtttgctta gattagttgt atggatgaaa
     3061 aagaaaaaga agaaaaatta aagcagctca ctcccgagga actgcatgtt actcaagaga
     3121 aggggactga aacaccattt cagaataagt actggaacaa tcaccagcag ggcatgtacg
     3181 catgtaaggt gtgcggagcg cagctatttg catcggacag caagtttgat tccggaaccg
     3241 gctggccgtc gtacgacaaa gctctacctg gagcagtaga atatgttgaa gatactagtc
     3301 atggtatgca ccgcgttgag gtggtctgca aaaactgtgg tgcgcatctt gggcatgtat
     3361 ttgatgatgg tccgactgac accaccagta aacggttctg tattaactcg tgcagtttag
     3421 attttaagaa agagaagtag ttcttagtta caacactaaa accgccacta tgtggcggtt
     3481 ttagtcagta cattcggtta ggatgggatg tctccgtcgg ggtattggtc ttcaaggatt
     3541 tgcttcatgt aaaacacgtc gtcagcttcc tcttcttctg cttcatcggt ttcatcaacg
     3601 agcggagcaa cgatgtcggt ttctccatct gcttcgagta agtcgattgg ctcttttgtt
     3661 ggagcgttta catcttcttc tcctaccagg gcactgtaat catttcccat attgttattg
     3721 gctttttata ttaaatgaat atgaaggtag ttgtagcaga aatgcttttt agttgtcaat
     3781 acctttgtgg aataattttt gaactcgcag ctcttcggca gcagcaagcc cgattgccat
     3841 cgcatcgatt tcgtcatcga ggagtgttgc aatgtctttt ttaagaattg tactaaccat
     3901 tttttgtact gattgcttgt cgctgttgcc cctgccagtt gccgagagtt ttacgagttg
     3961 tggcgacacc tcaagtactg caagccctgc ttcttcagcc gctaagagta tgactccgcg
     4021 cgcttcagct actttaatgg ctgtgcgctt gttgagcgaa aaaaagagtg tttcaaccgc
     4081 gagtacttcg ggggcatgca tcgtaatcgc ggtttgtatt ccgcgataaa tttcttggag
     4141 gcgcctgctg tgtgagtcgc ccttgctggt ggtgatgcag gttgaaaaga gcagttctgg
     4201 ttttgggctg catataaaca ctgcaatgcc gcagcggtcg tagcctgggt cgacagcgat
     4261 aattttttta ctctgcattg gtgtacactt cgttaacttc atcgctctct tcaagactct
     4321 caacgagttc gcccaatttt tccgcatctt catccgagag agggatagtt atagttggtg
     4381 tccatgtacc agcttctttg gtaaaccccc aggtaactga tccgatagcg cccaggctcc
     4441 cgccgtgttt tgtaaacgcg tgttttattt cagcagatgc tttgtttcgg ttcgcggtga
     4501 gtgcctgcac gacaatgccc acgccgccgg gaccatagcc ttcataggt
//
LOCUS       DATIBQ010000052        10773 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U16294
            k141_1856010, whole genome shotgun sequence.
ACCESSION   DATIBQ010000052 DATIBQ010000000
VERSION     DATIBQ010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935789
            Sequence Read Archive: SRR25158331
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 10773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:58:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 637
            CDSs (total)                      :: 603
            Genes (coding)                    :: 601
            CDSs (with protein)               :: 601
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10773
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1856010"
                     /isolate="SMAG_U16294"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..5948
                     /locus_tag="VLB02_01590"
     CDS             <1..5948
                     /locus_tag="VLB02_01590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56758.1"
                     /translation="AIDSDANTVAWNWDFTSAAVDSGLLISESSASTSGTQDQQALVE
                     ITTLATSTAAPLQITSNSTDVGDIFIDLASAGDFHIRNSGTAFVQFDDSGASAFGEGG
                     NYALISSTGNLQLFGSAEYLVAGNEFAFRYATDTAFGLKFNSSDSEYDFTTTGGATIF
                     AISAAASSGYVTLQNEGAFRYGDADGSNYVAFKSAATVGTNVTWTLPSADSSGCFQSN
                     GSGTISIASCGGAITADSLDFTDFSDSMTLDATTSIAFGASTLSLTFTNNGSGNEVHN
                     LSSTGDIVFQDNSSEFARFNDNGSVSFILDSTDNPDFSVTNQGSSGIIFDLTGTGDFL
                     VRDNGSTFLSVTDTGGFSLTLDSTDNPAYSLINNGSADVSTNLAGTGDFVIQDNGTAF
                     ATFSDSGMVGIGDTTPDDLLNIHSAAAAAGMAITSLGTDTDPYIKFELADGTGTFTMG
                     VDDSDSDKFKISTTALGTSDRLVIDSSGNVAVGTSTPLAKLHVGAGTDSPEDVISTIA
                     IFENAGASSLSVRDATSNVEAYIYADSGGVFFGSSTSSELHIGAAGNNHLTIATNGAA
                     TFSSTLAASNLSGTNTGNVTLGAIGSSANANGATLTGQVLNLEPASGSFGGVVTTGSQ
                     TFAGVKGLNTGLRVVGSTNDTAILSIETSQSTATSQLQIGGSTNIQYNMASRYNGIPT
                     ITSDYAFAQFVIGQNAVSENGTGTHPLLATMAIKPLTVTAGAAAVTNSATLYIENADN
                     AVTASGAYALWSDAGTNRFDGSTIFGTTISPQANDGAALGTGALAFSDAFLADGGVIN
                     FNNGNYTLTHSAGLLTANGALSIGTSNAFTTGTIELGAASDTTLARSGAGDVTIEGNA
                     IYRAGGTDVPVADGGTGVSTLASNGVLYGNGTGAIQALAVNAGATLCLTQASSAAPAW
                     GSCSGASGATAALDNLASVAINTSLVLGTSDGGALGSATKMWSDLFLASGGVINWNNG
                     DVTITHGADSLTFDGGGTVFNEAGGNFDFRIEGDTNANLFVADASADKVGIGTAAPAT
                     RLHVYKSIASNYDSQITMEEGQADAYAQISFIGTGREWHFGVGGGSETSFSVANKFYI
                     WDQQAAAMRLKIDTLGQLELPSLTLTDTGSQFDVNVTLGNDAGADTVSGINLDMTSVD
                     TGADSDVLQAITIANLSGGNANVTETAISIGTGWDTAINIGSGQIVTSNQGIEFTESD
                     TNPTCSSGNYTIYADTSEAKLKKCNNGVVSDLQAVPDMLNFEDTTSLPAIVDADTTEY
                     WNDTTRPNITPKTSADDVLVMVNVDITATSSTDTQLSMRIMRHTSAITCQSVGTALKQ
                     MSMFEGSAAGSFALSTVFVDSPGTASNVIYTLCSELDSEGAVGTLTSVEFTLFDINAT
                     TADLAEVYPTNDTSLKQGDVVSIDSMLTAGVKKSNGAYDKNVMGVVSTSPAMIIGSKG
                     SEGADGKPIALSGRVPVNVNMENGPIHKGDVLTASSTPGVAMRMTKAGMMIAIALEDY
                     NGEGVKPIMSFVKTGSYNGSNLKDILKGTVNDPAQLTQDAIGKTALQYFMQQKGAQLQ
                     PPLDVSEILADRVTAGLEVISPRVVTDQLAVNTVEVATGDAIKIKLGTGQQFSIFTAT
                     NEDEPTQTDTPIADTETPLVTIDALGNALFAGKITAKEIEAGKIKGLEMITSQLNILA
                     ESQEGSQQGLALTASALDALNAALGVVQTNVTELQEGVTQTTDVFSGLSATLESLSAR
                     ADALEALTASKTLADLESVTSGALTVSGVAQFSGEVVFEGGVSFKNIEFTVPPLFNAD
                     TAGFALIKTGDRRAKIEFEHEYVATPVVTAGVTFETEDALDDTAAEALFAENVQSLVV
                     EKSAAGFTILLNKPAPRDMRFSWIALAVRDPKVFESIFEGLTIESVLPPAPEPTQEEQ
                     AVPETSEIAPVEEPTTPETPAEGEAVATPEENAEPTTEAAPVPEETPTETVDVSAPEN
                     SAEESVPSEEVQPELVVPAEGGI"
     gene            5985..6482
                     /locus_tag="VLB02_01595"
     CDS             5985..6482
                     /locus_tag="VLB02_01595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56759.1"
                     /translation="MIKKTPTTTADSTDTTPSFIAARPPTRFFRKYFAWILIVLVIAL
                     AGATTYFYKKSRANPAQEEAKDLAAEVGRIILLPADELPTVATVSDPEALKSQAFFAD
                     AKKGDKVLIYTNAKKAVLYDPVAKKIINVAPLNIGESQKTSAVPKPVTVTAPTAEKTE
                     TTKKN"
     gene            6517..7524
                     /locus_tag="VLB02_01600"
     CDS             6517..7524
                     /locus_tag="VLB02_01600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56760.1"
                     /translation="MQTRTNLFKKIVALCAGFVLVFVSLARAGDLTGTNFIVRDPVIG
                     AGGGYQSSGSFKLYSSQDETFTGLGSSTSFKGNFGFLYYPYVQEGTLTTNLNGTNVDM
                     SWGATATALGFTVSGYKVGKATVSGGPYTYTNVGNVTSYTYTNLAAGTYYFVVETLDN
                     LSNTIAVSNEETETVPYTISFSISDNTIGFGTLSSVGARYATGDTTGTSSAAVAHTMT
                     VGTNASSGYSVSYRGPALSKAAKTISGATVTSDIDGTPGTEQFAIGVTTNGSATIATT
                     YQKSSNNWDFSSGTTTTVATQGAAVSNEVLSVYYLVNIAPVTDAGAYSTTVDYITTAN
                     F"
     gene            7629..8690
                     /locus_tag="VLB02_01605"
     CDS             7629..8690
                     /locus_tag="VLB02_01605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56761.1"
                     /translation="MKKFIRVLARITALFVVLPLLFPLTVQAAGVTSFSVTLSRLKAS
                     TAANQEIKFVTPTGLTAGQTITLTWSADFTMNTFDFADVDFATGDSSNCATASFSEQT
                     LAGTPSGATWGAALSSSTLTLTSGTGTVTASRCVRFRIGTNAVTGTTGDTQITNGAAD
                     DDDTVVVGGTFGDSGTAAVDIITDDQVSVTATVDPTITFTISDTTIGFGTLSSSAARW
                     ATGDTSGSGTDTSAHNLTVATNAASGYAVTYNGATLTKGSDTIDVASVNNDANGTPGT
                     EEFGLSVSTSGNATIATGYNHGDPDWTFVAGTTTTILSETVPTATETVSTYYLGNIAG
                     DTPAGAYSTTLTYIASANF"
     gene            8703..9719
                     /locus_tag="VLB02_01610"
     CDS             8703..9719
                     /locus_tag="VLB02_01610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56762.1"
                     /translation="MKKSIILLALALAVLLPRGAAFALTLSPPRWEVAADPGAMVTGS
                     IILINEESETRTYYASYENFEAQGDSGTPRFIGKRDGLATWLDAPASITLSAGESRAV
                     PFTITIPKDTEFGGYFAALFWGTTPPGTENGGQVAVGAKVGSLIFVTVGSNIEESAGL
                     LSFNTAAKRFFYKSLPVTFEYRFANDGADRVRPVGTITVRSMLGWRVAKVDANPFQGN
                     ILPGTTRKFSPEWTKLAPDERPSEAEKKAPYSFFKAVKNEWKNFAVGFFRAKMAIEYG
                     KTVQQLKSNNVYFIVFPFELMAILIIGTLILFFIFRRGVRRYNRWVISKAQARLVQMQ
                     QRGE"
     gene            9733..10587
                     /locus_tag="VLB02_01615"
     CDS             9733..10587
                     /locus_tag="VLB02_01615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="dockerin type I repeat-containing protein"
                     /protein_id="HSE56763.1"
                     /translation="METCSARLLGLLILFGLFVAPAYAATEVVTVTAKVGADAVPGGQ
                     SGGTAITGTRFFGAAYPGATVTLLKNTIERITTIADANGLFSMIDNQLDATAFYTLYA
                     VDAKGERSIILNYPLVAHQGYLTEVSGIQFPPTITLDKLEVREGDFLTVAGFALPRRL
                     LTISFSGPLHRNYSVQSESSGQYRMTLPLIDFPRGAYMVSSSYGGETQGSKVYQFTIG
                     TANVSQGEALQNIPGDVNFDGSVNLVDFSIIAFWYEKKNPPVVVDLNGDKKVDLADLS
                     ILAYYWTN"
     gene            10589..>10773
                     /locus_tag="VLB02_01620"
     CDS             10589..>10773
                     /locus_tag="VLB02_01620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56764.1"
                     /translation="MKKKLFYTLILFFLGTQNAFSASVPTLGIIAPASVTAHQQVAVA
                     IVLQNALVPINTATVSVR"
BASE COUNT         2833 a         2460 c         2614 g         2866 t
ORIGIN      
        1 cagcgatcga ttcagatgca aacacggttg cttggaattg ggactttact tcagcagccg
       61 ttgattcagg gctgcttata tcagaatcga gcgcttctac gagcggaaca caggatcagc
      121 aggcgcttgt tgaaataact acgcttgcta cctcgactgc tgctccgctg cagattacct
      181 ctaacagtac cgatgttggt gatattttca tcgacctcgc ttctgcgggg gactttcata
      241 tacgaaattc gggaacagcg ttcgtgcagt ttgatgacag tggagcctct gcttttggcg
      301 agggcggaaa ctatgcgctc ataagtagta ccggtaactt acagctcttt gggagtgccg
      361 aatatttggt tgctggaaat gagtttgcat tccgttacgc gactgatact gcgttcggct
      421 tgaaattcaa ttcatccgat agcgagtatg attttactac gaccggaggc gcaactattt
      481 ttgctatcag cgcagctgct tcgtcaggat atgtcactct tcaaaatgaa ggcgcattcc
      541 gctatgggga tgctgacggt agcaattatg ttgcctttaa atctgcagcc actgttggga
      601 caaatgttac ctggacattg ccttctgctg attcctcggg ctgcttccag tcaaacggct
      661 cggggacaat ctctattgca agctgtggcg gggcaattac tgctgacagc ttagatttta
      721 ctgactttag cgactcaatg acccttgatg ctacaacttc cattgccttc ggcgcttcta
      781 ctcttagtct cactttcact aacaacggat cagggaatga ggtgcataat ctttcttcaa
      841 cgggtgatat tgtctttcaa gataacagca gtgaatttgc ccggttcaat gataatggct
      901 ccgttagttt tatcctcgac agtactgaca atcctgattt tagtgttaca aaccaaggtt
      961 caagtggaat catttttgac cttactggta ccggtgattt cttggtccgc gataacggct
     1021 ccaccttcct ctccgttact gataccggcg gtttctcgct caccttagat agcaccgaca
     1081 atcctgctta ctcacttatc aacaatggtt ctgcagatgt gagcaccaac cttgccggca
     1141 cgggggattt tgtgattcaa gataacggaa ccgcattcgc aacgtttagt gactcaggta
     1201 tggtcggtat cggcgacacc acccccgatg atctcttgaa tattcatagc gcagccgctg
     1261 ctgcgggtat ggcaattact tcgcttggta ctgacaccga tccatatatc aaatttgaac
     1321 tcgccgatgg tacaggaact ttcaccatgg gcgttgatga ttctgattca gataaattca
     1381 agatttcaac tacagcgctc ggaacgagtg atcggttggt gattgattca agtgggaatg
     1441 tggcagttgg gacgagtaca ccgcttgcca aacttcacgt gggggcaggg acggatagcc
     1501 cggaagatgt aatctcaact attgctattt tcgaaaacgc aggagcatct agcctttctg
     1561 ttcgagatgc aactagtaac gttgaggcat atatctatgc cgattcagga ggagtatttt
     1621 ttggttcgtc aacaagtagt gagctgcaca ttggtgcggc ggggaataat catcttacta
     1681 ttgccactaa cggtgctgcc actttctcga gcacgcttgc tgcctcaaac ctctccggaa
     1741 cgaatacggg aaatgtaacc cttggtgcta tcggctcgag tgcgaacgcc aatggtgcaa
     1801 cacttacggg tcaagtttta aatcttgagc cggcttctgg ttcatttggt ggtgtggtca
     1861 caacaggatc gcaaacattt gctggggtca aaggacttaa tactggtctg cgagtagtgg
     1921 gaagtacgaa tgacacagca attctatcta ttgagacttc acagtcaaca gctacttctc
     1981 aactacaaat tggtggttca acgaatatcc aatacaacat ggcatcacgc tataatggta
     2041 ttcctactat taccagtgat tatgcatttg cacaatttgt tattggtcaa aatgcggtgt
     2101 ctgaaaacgg tacaggtacc catccgctgc ttgcgaccat ggccatcaaa ccgcttacgg
     2161 taactgccgg ggcagccgca gttaccaact ccgctacttt atatattgaa aatgctgata
     2221 atgcagttac tgcaagtggt gcgtacgccc tctggtccga cgccggcacc aaccgcttcg
     2281 atggctcaac tatcttcggc actaccatca gtccacaagc aaatgatgga gccgcacttg
     2341 gtaccggtgc actcgcattc tctgatgcgt tcttagcaga tggcggcgtc atcaacttca
     2401 acaacggcaa ctacacgctc actcattctg cggggctcct cactgcaaac ggtgccctct
     2461 ccatcggcac ctcaaacgcc ttcaccaccg gcaccatcga actcggtgct gcatctgata
     2521 ccacactcgc acgaagcggt gctggtgatg tgaccatcga aggcaatgca atataccgag
     2581 ccggcggtac cgatgtccca gttgcagatg gtggtaccgg tgtcagtacc cttgcctcca
     2641 acggcgtgct ctatggcaat ggtaccggtg caatccaggc acttgcggtg aatgctggtg
     2701 caacactctg tctgacgcag gcatcaagtg ctgcaccggc gtgggggtcg tgttcggggg
     2761 cgtcaggcgc taccgccgcc ctcgataacc ttgcttcagt agccatcaat acctcacttg
     2821 ttttaggaac ctcggacgga ggagcactcg gttcagcaac aaaaatgtgg agcgatctct
     2881 tcctcgccag tggcggtgtc attaattgga acaatggcga tgtcactata actcatggag
     2941 cagattcact tacctttgat ggtggtggta cggtatttaa cgaagcaggt ggtaactttg
     3001 acttccggat tgagggagat acgaatgcaa acctatttgt tgctgatgcg agcgcagaca
     3061 aggtgggtat cggcactgct gcaccggcaa cccggctcca tgtatacaag agtattgcca
     3121 gcaactacga ctcccaaatt actatggaag aaggtcaggc agatgcctat gcccaaatca
     3181 gctttatcgg taccggtcgt gaatggcact tcggagttgg cggtggttca gaaacctcat
     3241 ttagtgttgc aaacaagttc tacatctggg atcaacaggc agcagcgatg cgattaaaaa
     3301 ttgacacctt gggtcagcta gagttgccaa gcctaacact tactgatacc ggcagccagt
     3361 ttgatgtgaa cgttacgctt ggtaacgatg ctggtgctga tactgtttcc ggcattaacc
     3421 ttgacatgac gtcggttgat acgggagctg attcagatgt acttcaagca attaccattg
     3481 cgaatctttc agggggcaat gccaacgtga ctgaaactgc tattagtatc ggtactgggt
     3541 gggatacggc gatcaatata ggatcagggc agattgtaac ttcaaaccaa ggtattgaat
     3601 ttactgaatc tgataccaac ccaacgtgct cgtcggggaa ttacactatt tatgctgata
     3661 cttcagaagc aaaacttaaa aaatgtaaca atggtgtcgt gagtgatctt caagcagtac
     3721 cagatatgtt aaattttgaa gataccacat cacttcctgc aattgttgat gctgatacta
     3781 ctgaatattg gaatgacaca acaagaccaa acatcacacc aaaaacaagt gctgatgatg
     3841 tgctagtgat ggtgaatgtt gatattactg ctacatcgtc aactgatacg caattgtcga
     3901 tgagaattat gcggcatact tcagcaatta catgtcaatc agtaggtact gcactcaaac
     3961 aaatgagtat gtttgaggga agcgctgctg gttcatttgc gctatcaact gtgttcgttg
     4021 attctccagg taccgcgtca aatgttattt atactctttg ttcagaactg gattctgaag
     4081 gtgccgtggg aacgcttacc tcggttgagt ttactctttt cgatattaat gcgactactg
     4141 ctgacttggc cgaagtgtat ccgacaaacg atactagttt aaaacagggt gatgtagttt
     4201 ccattgacag tatgcttact gcaggagtta agaaaagtaa tggagcgtat gataaaaatg
     4261 taatgggcgt tgtttcaacg agtcctgcca tgataattgg aagtaaaggt agtgagggtg
     4321 cagatggtaa gccaattgct ctctctgggc gtgttccagt aaatgtaaat atggaaaatg
     4381 gtccaatcca taaaggagat gttcttactg catcttcaac cccgggtgta gcaatgagaa
     4441 tgacaaaagc tggaatgatg attgctattg ctctcgaaga ttataatgga gaaggggtaa
     4501 aacctatcat gtcatttgtt aaaactggtt cttataacgg ctcaaattta aaagatattc
     4561 ttaaaggtac tgttaatgat ccagcacagc taactcagga tgcgatcggt aaaactgcgc
     4621 tgcagtactt tatgcagcaa aaaggggcac agctgcagcc gccactcgat gtatctgaaa
     4681 ttttggctga ccgcgtaaca gcggggcttg aagttatctc accgcgtgtt gtcactgacc
     4741 agcttgcagt aaacactgtt gaagtggcaa ccggtgatgc gattaaaatc aagcttggca
     4801 ctggtcagca gtttagtatc tttactgcta ccaatgaaga tgagccgaca cagactgata
     4861 ctccaatagc ggatacagaa acaccacttg ttactattga tgcgttaggc aatgcactgt
     4921 ttgctggcaa gataaccgca aaagaaattg aagcaggtaa gattaaagga cttgaaatga
     4981 taacctcgca gttaaacatt cttgccgaga gccaagaggg cagccagcag gggcttgcac
     5041 ttactgcttc tgcgcttgat gcgctcaatg cagcgcttgg tgtggtgcaa acgaatgtta
     5101 ctgaactgca ggaaggcgta actcaaacta ctgatgtatt ttctggactt tctgcaacgc
     5161 ttgaaagttt aagtgcgcgt gccgatgccc ttgaagcgct cacagcaagt aaaacattgg
     5221 ctgatttaga atcagttacc agtggagcgc tcacggtgag cggtgttgca cagtttagtg
     5281 gagaggtagt atttgaaggt ggcgtgtcat ttaagaatat cgaattcacc gttccgccac
     5341 tctttaatgc cgacactgct ggttttgcac ttattaaaac gggggatcgc cgcgcgaaaa
     5401 ttgagtttga gcatgagtac gttgcaacgc cagtggtaac cgcaggtgtt acgtttgaaa
     5461 ctgaagatgc tcttgatgat acggcagcag aagcgctctt tgcagagaat gtgcaatcgc
     5521 tcgtggttga aaagagtgct gcagggttta ccattctttt gaacaagccg gcgccgcgcg
     5581 atatgcggtt ctcgtggatt gcgctcgcgg tgcgggatcc gaaagtgttt gagagtattt
     5641 ttgaggggct caccatagag tcggtactac cgcctgcacc cgagcctact caagaagagc
     5701 aagcagttcc agagacctca gagattgccc ctgtagaaga gcctacaacc ccagaaactc
     5761 ctgctgaagg tgaggcagtg gctactccag aagagaatgc cgaacccact accgaagcag
     5821 ctcctgtacc agaagaaaca cctactgaaa cggtggatgt ttcagcgcct gaaaattctg
     5881 cagaagaatc tgtaccgagc gaagaggtgc agcctgaact tgttgtacca gcagagggtg
     5941 ggatatagta ggtatggtac actggtcaaa tattttaatt ttgtatgata aaaaaaacac
     6001 ccacaacaac cgccgattct actgacacaa cgccttcgtt tatagcagct cgtccgccga
     6061 cgcgtttttt tagaaaatat tttgcttgga tacttattgt gctggtgatt gcgcttgccg
     6121 gagcaacaac ctacttctat aaaaagagcc gagctaaccc tgcccaagag gaagcaaagg
     6181 atttggctgc agaggtaggg cgcattattc ttttgccagc cgatgagctg ccgacggttg
     6241 ccaccgtttc tgatccagaa gcgctcaaaa gccaagcgtt ctttgcggat gcaaaaaagg
     6301 gggataaggt actcatttat acgaacgcaa aaaaagcagt gctctatgac cctgttgcaa
     6361 agaaaattat caacgtagca ccgttaaata ttggcgaatc tcaaaaaaca agcgctgtgc
     6421 caaagccagt tacggttact gcgccaactg ctgaaaaaac agaaaccaca aagaaaaatt
     6481 aatagcctcg cgtcggcttt ggtgtacaga ttgcacatgc aaacaagaac gaatcttttt
     6541 aaaaaaatcg tagcactgtg cgcagggttt gtgcttgttt ttgtgtcgct tgcgcgggca
     6601 ggggacctta ctggtactaa cttcattgtt cgcgatccgg tgattggtgc cggcggcggc
     6661 taccagtcat ctggcagctt caagctttac tcgtcgcaag atgaaacatt taccgggttg
     6721 ggaagttcaa caagctttaa aggtaatttt ggatttttat attatccgta tgtacaggaa
     6781 ggtacactga caactaactt aaacggtacc aatgtcgaca tgtcttgggg tgcaacagct
     6841 accgcgcttg ggtttacggt atcagggtat aaagttggca aagcaacggt ttcgggtgga
     6901 ccgtacacgt acacgaatgt agggaacgta accagctata cgtacacaaa ccttgcagct
     6961 ggtacttact actttgtggt cgagacactc gataacttaa gcaacaccat tgccgtttca
     7021 aacgaagaaa ctgaaaccgt gccctatact atttctttct ctatttccga taacaccatt
     7081 gggtttggta cgcttagctc ggttggtgcc cgctatgcaa cgggcgatac cacaggaacc
     7141 tcttcagcag ctgttgcgca taccatgacg gtaggcacta atgcttcatc tggatattcg
     7201 gtcagttatc ggggaccggc gctctcaaaa gcagcaaaaa caattagcgg cgccactgtt
     7261 acgagcgata ttgacggtac cccgggaacc gagcagtttg cgattggagt aactacgaac
     7321 ggcagtgcaa ctattgcaac tacctaccaa aaatcaagca ataactggga tttttcatct
     7381 ggtactacta ccacagttgc aacacagggt gctgcggtat caaatgaagt actttcagta
     7441 tattacctcg tgaatatcgc ccccgtaact gatgcgggtg cgtattcgac cacggttgac
     7501 tacatcacta ctgcaaattt ttaatggctg cttgatagag ttttttccac acaccgtatt
     7561 agtagttttg attacagggg aatggtacaa taaccatgtt attagggtat taaacgttga
     7621 agaatagcat gaaaaaattt attagggtgc tagcgcgtat caccgctctt tttgtggtgc
     7681 tcccgcttct gtttccgctc acggtgcaag ctgccggggt aaccagtttt tcggtaacac
     7741 tctcgcggct taaggcaagt acggctgcaa accaagaaat taaatttgtt accccaactg
     7801 gccttactgc cgggcaaacc attaccctta cctggagcgc tgattttaca atgaacacct
     7861 ttgattttgc cgacgtcgac tttgcgacag gggattcaag caactgtgca acggcaagct
     7921 tcagtgagca gacactcgcc ggtactccgt caggcgcaac gtggggggca gcactctctt
     7981 cttcaacgct tacccttact tcaggaacag gaacagtgac cgctagccgt tgtgtccgct
     8041 tccgtattgg tactaatgca gttactggta ctactggtga cactcaaatt accaacggtg
     8101 ctgcagatga tgatgacaca gtagttgttg gtggaacctt tggtgacagt ggtaccgctg
     8161 cagttgatat tattactgac gatcaagtct cagtaaccgc aacggttgac ccgaccatta
     8221 cgtttactat ctctgatacc actattggat ttggcacact cagttcttca gcagcgcgct
     8281 gggcaacagg agatactagt ggaagcggta ctgatacctc agctcataac cttaccgttg
     8341 caacaaatgc tgcttctggc tacgccgtaa cctataatgg tgcaaccctt actaaaggtt
     8401 cagatactat tgatgttgct tcagtaaaca acgatgcaaa cggtaccccg ggaactgaag
     8461 aatttggtct cagtgtttca accagtggta acgcaactat cgcaactggt tataatcatg
     8521 gtgatcctga ctggacattt gttgctggaa cgacaactac cattctttct gaaacagtac
     8581 caaccgcaac tgaaacagta agtacctact atcttggtaa cattgccgga gatactcctg
     8641 ctggtgccta ctcaacaaca ctcacctaca ttgcctcagc taacttttag taatactttt
     8701 tcatgaaaaa aagcatcatc cttctggcac tcgcgctcgc agtgctcctc ccgaggggtg
     8761 ctgcttttgc gctcactctt tctccgccac ggtgggaagt ggctgctgat ccaggagcta
     8821 tggttaccgg aagtattatt cttataaatg aagagagcga gacacgaaca tactacgctt
     8881 cgtacgaaaa ttttgaagca cagggagatt caggaacacc acggtttatt ggaaagcgtg
     8941 atgggcttgc aacgtggctt gacgcacctg cttctataac gctttcagcg ggagaatccc
     9001 gagcagtacc ctttactatt actattccaa aagataccga atttggcggt tatttcgcgg
     9061 cactcttctg ggggacgaca ccgccgggta ccgaaaatgg tggacaggtt gcggtgggtg
     9121 ccaaagttgg ctcgcttatt tttgtaaccg taggaagtaa tattgaagaa tctgctggat
     9181 tgctcagttt taatactgcc gcaaaaaggt ttttctataa atcgcttcca gtaacgtttg
     9241 agtatcggtt tgccaacgat ggtgccgacc gcgttcgtcc agtgggtacg attaccgtgc
     9301 gcagtatgtt aggctggcgg gtagcaaaag ttgatgctaa cccctttcaa ggaaatattc
     9361 tccctggtac tacccgaaaa tttagccccg agtggacaaa acttgctcct gatgagcggc
     9421 caagtgaagc tgaaaaaaaa gcaccgtact cgttctttaa agcagtaaaa aatgaatgga
     9481 agaacttcgc ggttggattt ttccgtgcaa aaatggcaat tgagtatgga aaaaccgtgc
     9541 agcaacttaa gagtaacaat gtctacttca tcgtatttcc attcgagctg atggctattc
     9601 tcattatagg aacacttatc ctcttcttta tattccgccg tggtgttcgt cggtacaacc
     9661 gatgggttat tagtaaggct caggcgcgtc ttgtacagat gcaacaacgg ggggagtagg
     9721 cgagtacggc gtatggaaac atgttccgcc cgtttactcg gtcttcttat cctcttcggt
     9781 ttatttgtgg caccggcgta tgctgctact gaagtggtaa ccgtaactgc aaaagttggt
     9841 gccgatgcag tgccgggcgg gcagagcggc ggcacggcga ttaccggaac tcgatttttt
     9901 ggtgccgcat atcctggcgc caccgttact cttttaaaaa acaccataga gcgaataaca
     9961 actattgctg atgcgaacgg actctttagt atgatagaca accagcttga tgctactgct
    10021 ttctacaccc tgtatgcagt agacgcaaag ggtgagcgtt caattattct taactatcca
    10081 ctggtagcgc accagggata cctaactgag gtgagcggta tccaatttcc gcccacgatt
    10141 acacttgata aacttgaagt gcgggaggga gattttttga cggtcgctgg gtttgcgctc
    10201 ccccgaagac tgcttaccat ttctttctcg ggaccattac atcgaaatta tagcgtgcag
    10261 tctgagagtt caggacagta tcgaatgacg cttccgctta ttgattttcc tcgaggtgcg
    10321 tacatggttt cgagcagtta tggtggcgaa acacagggga gtaaggtgta ccaatttacc
    10381 ataggaacgg caaatgtaag ccaaggcgag gcattacaga atattcctgg tgatgttaat
    10441 tttgatggct cggtgaacct ggttgatttt tcaattattg cattctggta tgaaaaaaag
    10501 aacccgcccg tcgtagtaga cttaaacggc gacaagaagg ttgatcttgc tgacttaagt
    10561 attcttgcct actactggac aaattaatat gaagaaaaaa ctattctata ctttaatact
    10621 cttttttctc ggtacacaga atgctttttc agcgtcagtg ccaacactgg ggattattgc
    10681 ccctgcatca gttacagcgc accagcaggt tgctgttgct attgtgttac aaaacgcact
    10741 cgtaccgata aatactgcca cagtatcggt acg
//
LOCUS       DATIBQ010000053         6523 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U16294
            k141_43066, whole genome shotgun sequence.
ACCESSION   DATIBQ010000053 DATIBQ010000000
VERSION     DATIBQ010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935789
            Sequence Read Archive: SRR25158331
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 6523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:58:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 637
            CDSs (total)                      :: 603
            Genes (coding)                    :: 601
            CDSs (with protein)               :: 601
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6523
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43066"
                     /isolate="SMAG_U16294"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2067
                     /locus_tag="VLB02_01625"
     CDS             <1..2067
                     /locus_tag="VLB02_01625"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HSE56765.1"
                     /translation="RDGRFGEWLREIKDWAISRERYWGTPLPVWVADDGERVVLDSIK
                     TLKKYSKKSGNTYFVMRHCQTENNLQKLWSFNLEREDVLTAEGKAMLQENVQLLADKN
                     IDLIVASPYSRTQQTAALVAEALGIPKENIITDERIGEWRTGDGFDGKPIEEYFKLRN
                     KGGDRYQFKLPGGESYADVLRRSGACLYDIDARYKNKNILFVSHGGSTRALMLHGEGV
                     SFDTLFASTRSFRNFDNAEIRALDFVPLPHNEDFELDLHRPYIDSVVLEKDSTVFRRV
                     KEVLDVWFDSGAMPFAQDHQPFSGEALRYPADFISEAIDQTRGWFYTLHAIGVLMGQG
                     KAYNNVICLGHLLDAQGKKMSKSVGNVVDPWEQIEKYGVDALRLWMYSVNQPGDSKNY
                     DEKTVDELVKKVFNLTYNVLSFYELYRDPSFEVDAENYEPVHVLDVWITARLNQFIAL
                     CTENLDTFKLFEPVRALRDFVDDLSTWYLRRSRERIKEGDRGAKQTLYIVLKTLAKVM
                     APFAPFTAEDLWQKLRNESDAESVHVAEWPKGNAFSVETLAEMQTVRDYCTTGNMLRK
                     KENIPVRQPLATFFIPTLLSELYQEIIKDELNVKHIRQGEVSLDTHITSELKAEGNLR
                     ELIRAVQDKRKEKNLQPKDRIALSLSAAYKETVVLFEEELKKAVAAEEIIFTEGEDIS
                     IELKKQ"
     gene            2112..2693
                     /locus_tag="VLB02_01630"
     CDS             2112..2693
                     /locus_tag="VLB02_01630"
                     /inference="COORDINATES: protein motif:HMM:NF023393.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein O N-terminal
                     domain-containing protein"
                     /protein_id="HSE56766.1"
                     /translation="MHEIHHTEGFILHTSDAGEANTLFHIFTKDLGRITATAQSLRRE
                     QSKLRGHLALYAHVALDVVQGRAVWRIIAARSLDDLPFSLPLPVRRLYGRTLEVLNRL
                     CQGEEPHEALFVHVAEFTALIRERALSVVERKFLDTISVVRMLSALGYLEQDAAVHIA
                     TSGTLAEALARIDEASNKILIERAHEAFKASHL"
     gene            2713..4614
                     /locus_tag="VLB02_01635"
     CDS             2713..4614
                     /locus_tag="VLB02_01635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56767.1"
                     /translation="MPDDGKLDHIAELQKKLYARDQSGLPERRIGILRPIKTSVQSTW
                     GSGTPTIPKSKKKQILGSYRKFFLFSIIFLVLSMFVLGYAVLRGGNTLSAKNVDVTIL
                     GNSFIAGGESLPVAVEVANRNAVALLEAVLTIEYPKGSLDASGAEVIRIRKDLGTVPA
                     GKAKSESFEAVLYGEQGTERTIKARLEYRLPNSNTPFVKEGTLAVSISSSPVNLTIDG
                     PKTVPANQPVTFTIQTELIAEQANKDMILKIDYPTGFKYQSSSVAPISGNNVWSLAAL
                     TKGSPKAITIEGIAGGQSGEEKSFRVYTGAQDAENPTKFSVVYSSALHTIALESAFLE
                     GHITLGDRVGSVVAVEKGTELSGYVQWNNNLEEQLVGPEIVLAISGVALDESSIRSTG
                     GFYDSKRKAIVWNSGTTTNFESLAPGASGQFPFSFKTVDAAVGRRASDITLALSIRGR
                     ASESGFEDRVVDAIDKLTVRFIGSINFAASNSYKTGPIKNNGPVPPRADQTTTYTITW
                     TVRPGDVAVQNAQATATLPFYVAWAGVVSPTNEAVTFEQESRTVRWRIGTIPQSVGVA
                     QSRSASFQVSVTPSRSQVGSDLTLTNEGGVSGVDSVTSSLLQLSVPALTTRIDNLLLG
                     EIADGVVAP"
     gene            4620..5930
                     /locus_tag="VLB02_01640"
     CDS             4620..5930
                     /locus_tag="VLB02_01640"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HSE56768.1"
                     /translation="MELPTLETIVSLAKRRGFIFPGSEIYGGLAGTWDFGPLGVALKN
                     NIKESWRKKFVLGRDDMYEIDAAILMNSKVWEASGHVAGFSDPMVECKKCKSRFREDQ
                     LDSNECPTCGTKEAFAEARQFNMMFKTQAGAVEDSSATVYLRPETAQGMFVNFKNIID
                     TFHPKLPFGMAQIGKAFRNEITPRDFVFRSREFEQMEIEYFIQETHWEKYFTYWKDEM
                     VEWMQTIGLAMDRVHEITKGADELAHYSKSSTDFEFDFPFGQKELFGISYRTDFDLKQ
                     HMQHSTVSLEYSEEGEQKFVPHVIEPTFGVERAVLAVLVSAYCEEELDNGEKRVYLKL
                     PPKIAPVKICVSPLLKNKPELVEKAKLIHGSLKEVFGAVAWDDNGNIGKRYRRQDEIG
                     TPFCVTIDFDTLTDESVTVRNRDTGSQERIALAELHTWFTEKLQ"
     gene            5973..>6523
                     /locus_tag="VLB02_01645"
     CDS             5973..>6523
                     /locus_tag="VLB02_01645"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HSE56769.1"
                     /translation="MDSLKTIRVSSDTFVRLFLIALAIYGLFLIRDILILILVAIVIA
                     SFVESGVRYLSRYRLRRTLSVSIIYTISILILFAIFYAFVPIIFRELSGVLSLISQYL
                     PSSNLDSQSIEGATQLVTTISQKGSFADLLGNIKSLAVTFSAGFTSVIGGTFGGVVNI
                     ILVFVMSFYLSVQERGIETFLRIL"
BASE COUNT         1760 a         1440 c         1630 g         1693 t
ORIGIN      
        1 cgtgatgggc ggtttggtga atggcttcgt gagattaaag attgggcaat ttctcgcgag
       61 cggtactggg gtacaccgct gccggtgtgg gttgcagacg acggcgagcg cgtggttcta
      121 gattcaatta aaacacttaa aaaatattct aagaaaagcg gcaacacgta ttttgtgatg
      181 cgtcattgcc aaacagaaaa taacttacag aagctctgga gctttaatct tgagcgtgaa
      241 gatgtattaa ctgccgaagg caaggcaatg ctgcaagaaa atgtacagct gcttgccgat
      301 aaaaacattg acctcatcgt ggcttcgccg tactcgcgca cccagcaaac agcagcgctg
      361 gtagccgagg cgctcggtat tccaaaagag aacattatta ccgatgagcg tattggtgaa
      421 tggcgcaccg gcgacgggtt tgatggaaag ccaattgaag agtattttaa acttcgaaat
      481 aagggaggtg atcgatacca atttaagctg cctggcgggg agtcgtatgc tgatgtattg
      541 aggcgctctg gagcgtgctt atacgacatt gatgccagat ataaaaacaa aaatatttta
      601 tttgtttctc atggcggttc tacccgagca ctgatgctcc acggtgaagg cgtttcgttc
      661 gatacgcttt ttgcgagcac gcgaagcttt cgcaattttg ataatgctga aattcgcgcg
      721 cttgattttg tgccgctgcc gcataacgaa gactttgaac tcgacttaca tcgaccctat
      781 atcgactccg tcgtcttgga aaaggacagc acggtatttc gccgggtaaa agaagtgctc
      841 gatgtatggt ttgattcagg ggcgatgccc tttgcgcaag accatcagcc tttttctggg
      901 gaagcattgc gctacccggc ggattttatt tcagaagcaa tcgatcaaac ccgcggctgg
      961 ttctacacgc tccatgcaat tggcgtactg atggggcagg gtaaagcgta taacaatgta
     1021 atttgtttag gacatctgtt ggatgcacag gggaagaaga tgtcaaagag tgtggggaac
     1081 gttgttgatc cgtgggagca gatagaaaaa tatggcgtgg atgcgttgcg cctgtggatg
     1141 tactcagtca accagccggg ggactcaaaa aattatgacg agaaaacagt tgatgaactg
     1201 gtgaaaaaag tctttaacct aacgtacaac gtactttcgt tttatgagct ctatcgtgac
     1261 ccatcatttg aagttgatgc agaaaactac gaaccagtac atgtacttga tgtatggatt
     1321 actgctcggc taaaccagtt tattgcactc tgtaccgaga atctggatac ctttaaacta
     1381 tttgaaccag tacgtgcact ccgtgatttt gtggatgatc tttcaacctg gtacctgcgc
     1441 cgctcgcgcg aacgtataaa ggaaggagac aggggagcga agcaaacgct gtatatagta
     1501 ttaaaaacac tcgcaaaagt tatggcacca tttgcgccgt ttactgccga agatctctgg
     1561 caaaaattac gaaatgaatc cgatgcagaa agtgtgcatg tagcagagtg gccgaaagga
     1621 aacgccttta gtgtagagac acttgcagaa atgcaaacag ttcgtgatta ctgtaccacc
     1681 ggaaacatgc tcagaaaaaa agagaatatt ccagttcgtc aaccgctcgc tacctttttt
     1741 attcccactc ttctttccga gctgtatcag gaaattataa aagatgagct aaatgtaaaa
     1801 catatacggc aaggagaagt atcactagac acacatataa cttctgaact caaagccgaa
     1861 ggaaatctcc gcgaactcat ccgcgcggtg caagataaac gcaaagaaaa aaatttacaa
     1921 ccaaaagatc gtatagccct ttcactctcg gctgcataca aagaaaccgt tgtgctgttt
     1981 gaagaagaac tcaaaaaagc agtcgcagca gaagaaatta tctttactga aggagaagat
     2041 atttctatcg agcttaaaaa gcagtaacgc tttttgatta ttgtttattg aagcttgatt
     2101 attactctac tatgcatgag attcatcaca cggagggctt cattctccac acgagcgatg
     2161 cgggggaggc aaacacgctt ttccatattt ttaccaaaga tcttggtcgg ataactgcta
     2221 cggcacagtc gctcaggcgt gagcagtcaa agttgcgcgg gcatttggcg ctctatgccc
     2281 atgtggcgct cgatgtagtg cagggcagag cggtgtggcg cattattgcc gctcgttcgc
     2341 ttgatgactt gccattttca cttccgttgc cggtgcggcg cctgtatggg cgcacacttg
     2401 aagtgctcaa tcgtttgtgc cagggcgaag aaccgcacga ggcactgttt gtacatgtag
     2461 cagaatttac agctttgatt cgcgagcgag cgctctctgt ggttgagcga aaatttttag
     2521 acacaatttc agtggtgcgc atgctctctg ctctagggta cctcgagcag gatgcagcgg
     2581 tacatatagc aacgagcgga acacttgcag aggcgctggc gcgtatagat gaagcatcaa
     2641 acaaaatact gattgaacgc gcacacgaag cattcaaagc atcgcatttg taaatgctat
     2701 actctgtaac gtatgcccga tgatggcaaa ctcgatcaca ttgccgagct ccagaaaaaa
     2761 ctctatgctc gcgaccaaag cggcttgcct gaacgccgta ttggtatttt gcgtcccata
     2821 aaaacaagtg tacagtcaac ctgggggagc ggtacgccga caattccaaa atctaaaaaa
     2881 aagcaaatac tgggaagtta tcgaaagttt tttctgttct caattatttt tttagtactc
     2941 tcgatgttcg tgctcggcta cgcagtgctt cggggcggca acaccctttc ggcaaaaaat
     3001 gttgatgtta ccattctcgg aaactcgttt attgcgggcg gagaatcgtt gccggtagca
     3061 gtcgaggtag cgaatcgaaa tgcagttgca ctgctggaag cggtactcac tattgaatac
     3121 cctaaaggtt cactcgatgc atccggcgca gaggtaattc gaattcgcaa agacttggga
     3181 acagtacctg ccggaaaagc gaaaagtgaa tcattcgaag cagtgctcta cggcgaacag
     3241 ggaaccgagc gaaccattaa ggcgcgcctc gagtatcggc taccaaactc gaatacgccg
     3301 tttgtaaaag agggtaccct cgctgtttcc atcagctctt caccagtgaa tctcaccatt
     3361 gatggtccaa aaacagtacc agcaaatcag ccagtaacgt ttaccattca aaccgaactt
     3421 attgccgagc aggcaaataa agatatgatt ttaaaaatcg attatccgac aggatttaag
     3481 tatcaatctt caagcgttgc ccctatttca ggaaataatg tatggtcgct cgcagcactc
     3541 acgaaaggat caccaaaagc aattaccatt gaaggcattg cgggtgggca gagcggagaa
     3601 gaaaaatcgt ttcgggtgta taccggtgca caagacgctg agaatccaac caaattttcg
     3661 gtggtgtaca gttctgcact ccacactatt gcgcttgaga gtgcatttct tgaaggacat
     3721 attacgctcg gcgatcgggt cggcagcgtg gttgcggttg aaaaagggac agaactttca
     3781 gggtacgtgc agtggaataa taatttagaa gaacagctgg ttggtcctga gatagtgctt
     3841 gctatttcag gagtagcgct cgatgaatca tcaattcgtt caacgggcgg attttacgat
     3901 tcaaaacgaa aagcaattgt atggaatagc ggcacgacta ctaattttga aagccttgcc
     3961 ccgggggcga gcgggcagtt tccgttctca tttaaaacag ttgatgcggc ggttggtcgt
     4021 cgggcgagtg atattaccct ggcactctca attcgcgggc gagcgtccga gtcggggttt
     4081 gaggaccgtg tcgttgatgc gattgataag ctcaccgttc ggtttattgg ctctatcaat
     4141 tttgcggcta gtaacagcta taaaacagga ccaattaaaa acaacggtcc agtaccgcca
     4201 cgcgcagatc aaacaacaac ctataccatt acctggacag ttcgcccggg tgatgtagcg
     4261 gtgcaaaatg ctcaggcaac tgcaaccttg ccgttctacg ttgcctgggc aggcgtggtg
     4321 tcgccaacga atgaagcggt tacttttgaa caggagtctc gaacagttcg ctggcgcatc
     4381 ggcactattc cgcaatcggt cggcgttgca cagtcgcgga gcgcttcatt ccaggtttca
     4441 gttactcctt cacgctcgca ggtgggtagt gatcttaccc tcacaaatga agggggtgtg
     4501 tcgggcgttg acagtgttac cagcagcctg ctacaactaa gcgtgcctgc gcttaccacc
     4561 cggatcgaca atctcctgtt aggagaaata gccgacgggg tggtagcacc gtaactacta
     4621 tggaactgcc tacactagaa acaatcgtct cactcgccaa gcgccgcggc tttatttttc
     4681 ccggatctga aatttatggc ggacttgccg gtacctggga ttttggtccg ctcggtgtcg
     4741 ccctcaaaaa taatataaaa gaatcatggc gcaaaaagtt tgtgctcggt cgtgatgata
     4801 tgtacgaaat tgacgcagca attttaatga actcaaaagt gtgggaggcg agcgggcatg
     4861 tggcaggatt ctctgatccg atggtggagt gtaagaagtg caagtctcgt ttccgagaag
     4921 atcagctcga ttctaatgaa tgccctactt gtggaactaa agaagcattt gccgaggcgc
     4981 ggcaattcaa tatgatgttt aaaacccaag ctggtgcagt tgaggattca tcggcaacgg
     5041 tgtaccttcg ccccgaaact gcccaaggga tgtttgtgaa ttttaaaaat attatcgata
     5101 cctttcaccc aaagttgccc tttggtatgg cacaaattgg taaggcattt cggaatgaaa
     5161 ttactccgcg tgattttgtg ttccgctcgc gcgagtttga acagatggag atcgaatatt
     5221 ttatccaaga aactcactgg gaaaaatatt ttacctactg gaaagatgaa atggtggagt
     5281 ggatgcaaac tatcgggctg gcgatggata gagtacacga aattaccaaa ggtgccgacg
     5341 agctagcaca ctactcgaag agcagtaccg attttgaatt tgattttccg ttcggacaaa
     5401 aagagctctt tggtatttca taccgaaccg actttgattt gaagcagcac atgcagcact
     5461 cgacagtttc gctcgagtac tcagaagaag gagagcagaa atttgtaccc cacgttattg
     5521 agcctacctt tggtgttgag cgtgccgtac ttgccgtact cgtgagtgcc tattgcgagg
     5581 aagagctcga taacggcgag aagcgtgtct atttaaaact accacccaaa attgcgccgg
     5641 taaaaatctg cgtgtcgcca ctccttaaaa ataaaccaga actagttgaa aaagcaaaac
     5701 ttattcatgg atcgctaaag gaagtatttg gcgcagttgc ctgggatgat aacggtaaca
     5761 ttggtaagcg ctaccgccgc caagatgaaa ttggcacacc attttgcgta actattgatt
     5821 ttgacacgct gaccgacgaa tcagtaacgg tgcgtaatcg tgataccggg agccaggaac
     5881 gtattgcgct tgccgagctc catacctggt ttactgaaaa actccaatag gcgcagcttg
     5941 ttcttggggg tgcccctgtg tatgataggg ttatggattc tcttaaaacc atccgcgttt
     6001 caagcgatac atttgtccgg ctgttcctca ttgctctggc aatatatggc ttattcttaa
     6061 ttcgagatat tctcattctt attttggtag cgatcgtcat tgcctcgttt gttgaatctg
     6121 gggtgcggta tctctcccgc taccggctac gacgcacgct ctcagtttcg attatttaca
     6181 ccatttctat tcttatttta ttcgctattt tctatgcatt tgttcctatt atcttccgcg
     6241 agctttcggg ggtattgagc ctcatctcgc agtacttgcc gtcatcgaac cttgattcac
     6301 aatcgattga aggagcaacg cagctagtaa cgaccatttc acagaagggc tcatttgctg
     6361 acttgctcgg caatattaag agcttggcgg ttactttttc ggcagggttt acctcggtta
     6421 ttggcggcac gtttggcggg gtggtaaata ttatcctcgt cttcgtgatg tcgttctacc
     6481 tctcggtgca agagcggggg attgaaacct tcctgcgcat tct
//
LOCUS       DATIBQ010000054         4029 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U16294
            k141_15602, whole genome shotgun sequence.
ACCESSION   DATIBQ010000054 DATIBQ010000000
VERSION     DATIBQ010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935789
            Sequence Read Archive: SRR25158331
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 4029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:58:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 637
            CDSs (total)                      :: 603
            Genes (coding)                    :: 601
            CDSs (with protein)               :: 601
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4029
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15602"
                     /isolate="SMAG_U16294"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..890
                     /locus_tag="VLB02_01650"
     CDS             <1..890
                     /locus_tag="VLB02_01650"
                     /inference="COORDINATES: protein motif:HMM:NF025017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HSE56770.1"
                     /translation="NYGIDQTKAMFITCLDADSFVAPDALKRMLYTFQEHPEVMAVAP
                     SIIINQPKNILQSIQRVEYNMSLYIKKMLSFLNAIHVTPGPFSVFRREVFERIGKFRK
                     AHNTEDAEIAFRMQVNHMKIEHCHLAQVYTVAPNTVGKLFRQRLRWIYGFIQNVIDYR
                     YVIFRKKYGNFSMFTIPSGIISIATIIYIFLSLLYHTSVSAYEKMVQINTVGISSSFH
                     LPHFDLFFMNTRATALVSTVLYCFVILSVIQGYRLARERNMVSWYLIPYMVVYSILTP
                     LWLMKAVWNSITAQKPKWR"
     gene            900..1598
                     /locus_tag="VLB02_01655"
     CDS             900..1598
                     /locus_tag="VLB02_01655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56771.1"
                     /translation="MGRGTKYVFVFILTCGVFAISWYVSSYFSQQRIAEIRNIQNKVA
                     TDILSSETDFSLLQELSCQDLNRTVTSEELGSLASKISFSEQSANAQDEILLLKKQYS
                     ILEVKDFLLKKRVSERCKTPLTTIFYFYSTEDDCDDCVKQGYVLDAIREKYPDVHIYS
                     FDYNLDLSTIRALRLIYKIADPLPVLVVNGKTYNGFNTVEAVESLLPKEFIKQEQQKT
                     LKAEISYNTTTLLN"
     gene            complement(1659..1731)
                     /locus_tag="VLB02_01660"
     tRNA            complement(1659..1731)
                     /locus_tag="VLB02_01660"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1696..1698),aa:Thr,seq:tgt)
     gene            complement(1795..2193)
                     /locus_tag="VLB02_01665"
     CDS             complement(1795..2193)
                     /locus_tag="VLB02_01665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56772.1"
                     /translation="MLLLTVSFVFLALCLYVALSYRAWQMQQGLVTLHTTPEQTTRAA
                     VRVLSHKAEAFLKAALHVCVYYALEIGKLASNEVKSWVFKAFPRLKIALTEKDKLTGL
                     YHGPASFFLANVSEYKTETVRTPQQRRKVL"
     gene            complement(2196..2975)
                     /gene="tsf"
                     /locus_tag="VLB02_01670"
     CDS             complement(2196..2975)
                     /gene="tsf"
                     /locus_tag="VLB02_01670"
                     /inference="COORDINATES: protein motif:HMM:TIGR00116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HSE56773.1"
                     /translation="MITTEQVKTLRDQTGVSVMQCKKALEETNGDLEKALVILRKKGT
                     EIAAKKADRSAADGLIVTRAANGKALTLILNCETDFVARNEGFVVLANTLADIAVQEG
                     AAAAQEKAPALISDVVLKIGENIQLGSIDELAGPVVNFYTHHTGKVGAVVVLSVGTEA
                     LAKDIAMHITAMKPTYLSEADIPASAKESVLEVFKKEVDESGKPEEVKAKMLEGKIAT
                     YFKEQTLLDQPFFKDGDKSIAQLLKDNGNAAIITYRLYTVG"
     gene            complement(3001..3672)
                     /gene="rpsB"
                     /locus_tag="VLB02_01675"
     CDS             complement(3001..3672)
                     /gene="rpsB"
                     /locus_tag="VLB02_01675"
                     /inference="COORDINATES: protein motif:HMM:TIGR01011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HSE56774.1"
                     /translation="MQFNVTTMFEAGAHFGYSKTRRHPTVVPYLFGSKNGIDIIDIEK
                     VHPLLEKAASVLADLGKAGKTVLFVGTKAEAKGAVESVAVALRQPFVTERWIGGILTN
                     FPEIKKRIQKLEEMKEKRANGEYDKYTKKEKILLNRELDRLNKYFSGLVGMMKLPDAI
                     VVVDTKKEHIAVTEAKKMGLTVIGIGNTDCSVKSVHYPIVANDGSRSSIELVLDILKA
                     AYEQQ"
     gene            3805..>4029
                     /locus_tag="VLB02_01680"
     CDS             3805..>4029
                     /locus_tag="VLB02_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase"
                     /protein_id="HSE56775.1"
                     /translation="MGIPMRQTELFTKTKRENPTDEVSKNAQLLIRGGYIYKEMAGVY
                     ALLPLGLRVFNNIIRIIREEMNTIGGQELVL"
BASE COUNT         1088 a          878 c          881 g         1182 t
ORIGIN      
        1 taaactacgg gattgatcaa acaaaagcga tgtttatcac ttgcttagat gccgattcct
       61 ttgttgctcc tgatgcgctc aagcggatgc tctatacctt ccaagaacat cctgaggtta
      121 tggcagtggc gccttcaatt atcatcaacc agccaaaaaa tatactgcaa tcgatacagc
      181 gggttgagta caacatgagt ctctacataa aaaagatgct ctcgtttttg aatgctatcc
      241 atgtaacccc gggacccttt tctgtattta ggcgcgaggt attcgagcgc attggaaaat
      301 ttagaaaggc gcataatacg gaagatgctg agatcgcatt tcggatgcag gtgaaccata
      361 tgaagattga acactgtcac ctggcgcagg tatatactgt tgcacctaat acggttggta
      421 aactattccg acagcggctc cgctggatct acggttttat acagaacgtt attgattacc
      481 ggtatgttat ttttagaaaa aagtatggta atttttcgat gtttacgatt ccctcgggca
      541 tcatttcaat tgcaacaatt atctacattt ttttaagcct actttatcac accagtgttt
      601 ctgcctatga aaaaatggtg cagattaaca cggtaggtat ttcgtcatcg ttccacctgc
      661 cgcactttga tctctttttt atgaatactc gggcgacagc actggtgagt accgtgctct
      721 actgttttgt tatcctcagt gtcattcaag ggtatcgcct tgcgcgcgag cgaaacatgg
      781 taagctggta tcttattccg tatatggttg tgtatagtat tcttacaccg ctctggctta
      841 tgaaggcagt atggaacagt atcactgccc aaaaaccgaa gtggcgatag agttataata
      901 tgggaagagg tacgaaatat gtatttgttt ttattctcac ctgcggggtg tttgccattt
      961 catggtacgt gagctcgtac tttagccagc agcgcattgc tgaaatcaga aatattcaaa
     1021 ataaagttgc taccgacatc ctttcatctg aaactgactt ctcgcttctc caagaacttt
     1081 catgtcagga tttaaaccga acggttactt cggaagagct cggcagtttg gcgagtaaaa
     1141 tctcgttcag cgagcagagc gcaaatgcgc aagacgaaat cttgctcctc aaaaagcagt
     1201 actctatttt ggaggtgaag gattttttgt tgaagaagcg ggtgagtgag cgctgcaaaa
     1261 caccgctcac cactattttt tacttttata gtaccgaaga tgactgtgat gattgtgtaa
     1321 agcaggggta cgtgctcgat gccattcgag aaaaatatcc cgatgtacac atttactcgt
     1381 ttgactacaa cctcgatctc tctaccatcc gcgcacttcg tttaatttac aaaattgctg
     1441 atccattgcc agtgctcgtg gtaaacggta aaacctacaa tggctttaat actgttgaag
     1501 cggttgagtc cctcctgcca aaggagttta taaaacagga acagcaaaaa acgcttaagg
     1561 cagaaatttc atacaatact acaaccctat taaattagac tggagaatcg aagctgtcac
     1621 agaaaaggtg aaagaaaaat taagggttta gtacattgag ccgcttgtca gacttgaact
     1681 gacgaccttc gctttacaaa agcgttgctc taccaactga gctaaagcgg cactctattg
     1741 cttttttcac tgaaaaccaa gggcaagtat gccggaagaa aacattcttt gcaactataa
     1801 tacctttcgg cgctgctggg gagtgcgaac agtctctgtt ttatactcgg aaacattcgc
     1861 caaaaaaaac gatgccggac cgtggtacaa accggtgagt ttgtctttct cggtaagggc
     1921 aattttcaag cgggggaatg ctttaaacac ccaggacttt acctcgttac tggcgagttt
     1981 tcctatttca agagcatagt atacgcacac atggagagca gctttcaaaa aagcttcagc
     2041 tttgtgtgac aacactctca ctgctgctcg agtggtttgt tctggggtag tgtgtagggt
     2101 aacaagcccc tgttgcatct gccacgcacg ataagagagt gcaacgtaga ggcagagcgc
     2161 caaaaaaaca aaagaaacag taagtagtaa cataattagc ctacggtgta gagcctatag
     2221 gtaatgatag cggcattacc gttatctttc aagagttgcg cgattgattt atccccatcc
     2281 ttgaaaaaag gctgatccag taaggtttgt tctttaaagt aggtagcaat tttgccttca
     2341 agcatctttg cttttacttc ctcaggcttg cccgattcat caacctcttt tttgaacacc
     2401 tcaagcacac tctcttttgc agatgcagga atgtcggctt cagaaaggta cgtcggcttc
     2461 atggcagtaa tgtgcatcgc aatatctttt gccagtgctt cagtgccgac actgagcact
     2521 actaccgcac caactttgcc ggtgtggtgg gtgtagaagt tcaccactgg accagcaagt
     2581 tcatcaatcg aaccgagttg aatgttctcg ccaattttta ggacgacatc agaaataagt
     2641 gcaggggctt tctcttgcgc agcggcggct ccttcttgta ccgcaatatc ggcaagggtg
     2701 tttgcaagca caacaaatcc ttcattgcga gcaacaaagt cagtctcaca gttaagaatt
     2761 agggtgagtg ctttaccgtt tgctgctcgg gtaacaatca agccgtcagc agctgaacga
     2821 tcggcttttt ttgcagcaat ctctgttccc ttcttacgga gtataacgag cgctttctct
     2881 aagtcgccgt ttgtttcctc aagtgctttc ttgcactgca taaccgacac gccggtctgg
     2941 tctcgtaatg ttttaacttg ctcggtggtg atcatagttt gatttgagtt tgttatttgg
     3001 ttattgctgc tcgtaagcag ctttgagtat atcaagaacc agctcaatac tcgaacgtga
     3061 gccgtcattt gcaacaatag ggtagtgtac gctctttact gaacaatcgg tattgccgat
     3121 accaatcacc gtaagtccca tcttttttgc ttctgttacg gcaatatgct cttttttagt
     3181 atcaacaact acaatcgcat cggggagctt catcatacca actaatccac tgaaatattt
     3241 attcaagcgg tcaagttcac ggttcaaaag aattttctct ttttttgtgt acttatcgta
     3301 ctcaccgttt gcacgcttct ctttcatttc ctcgagtttc tgaatgcgtt tcttgatttc
     3361 aggaaagtta gtgagaatgc ccccaatcca gcgttcggtg acaaacggct ggcggagcgc
     3421 aacagcgact gattcaacag cgccctttgc ctcggccttg gtgcccacaa aaagcaccgt
     3481 ttttccggct ttgccaaggt cggcaagtac tgaagcggct ttttcaagca gggggtgtac
     3541 tttctctata tcaataatat cgataccgtt cttactaccg aagaggtagg gaacaacggt
     3601 tgggtggcgg cgggtttttg aatacccaaa gtgcgcacct gcttcaaaca tggtagttac
     3661 attaaattgc atatcgtttc tgcggcagaa gggctcgttt cgtccctcct taattaatta
     3721 ttaacgaggc ttgaaatata ccatttctta tataaaaagc aaacaaagta tattctcttt
     3781 ttttattatt gtttttatgc gattatggga atacctatgc gccaaacgga gctatttacc
     3841 aagacaaaac gggagaatcc tactgacgag gtttctaaaa atgcacaact gcttattcgg
     3901 ggcgggtaca tatataaaga aatggcaggg gtgtatgcac ttttacctct cggactgaga
     3961 gtctttaaca atattattcg tatcattcga gaagagatga acactatcgg cgggcaggag
     4021 ctcgtactt
//
LOCUS       DATIBQ010000055         3288 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U16294
            k141_1286806, whole genome shotgun sequence.
ACCESSION   DATIBQ010000055 DATIBQ010000000
VERSION     DATIBQ010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935789
            Sequence Read Archive: SRR25158331
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:58:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 637
            CDSs (total)                      :: 603
            Genes (coding)                    :: 601
            CDSs (with protein)               :: 601
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3288
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1286806"
                     /isolate="SMAG_U16294"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            71..493
                     /gene="rpsL"
                     /locus_tag="VLB02_01685"
     CDS             71..493
                     /gene="rpsL"
                     /locus_tag="VLB02_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HSE56776.1"
                     /translation="MPTLNQLIRNKRSKPKRKSKAVALSRGFNAIKNKPVFYPAPFKR
                     GVCVKVSTTTPKKPNSAIRKIARVRLTNSQEVTAYIPGIGHNLQEHSVVMIRGGRVKD
                     LIGVRYHIVRGVLDTQGIDKRRVSRSLYGTKRPKEAKK"
     gene            578..1048
                     /gene="rpsG"
                     /locus_tag="VLB02_01690"
     CDS             578..1048
                     /gene="rpsG"
                     /locus_tag="VLB02_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007526570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HSE56777.1"
                     /translation="MRRPVKNRNIVGEDMRYGSVKLEKFINSIMQDGKKNTARTIVYN
                     ALEVLKEKEKVENPLEVFDAALKNAGPLVEVRSRRIGGANYQVPREVRPERRTALAMR
                     WIIESTRNRKGVKAAVALADELSQAAKGEGNAVKKKKDTHKMAESNKAFAHFTW"
     gene            1295..>3288
                     /gene="fusA"
                     /locus_tag="VLB02_01695"
     CDS             1295..>3288
                     /gene="fusA"
                     /locus_tag="VLB02_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009499060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HSE56778.1"
                     /translation="MNRDYPLEKVRNFGIIAHIDAGKTTTSERVLYYTGMSHKIGEVH
                     EGETVTDWMEQERERGITITAAAVTCTWIPTYTAGDKSKQYRFNIIDTPGHIDFTVEV
                     KRSLRVLDGAVVVFDGVAGVEPQSETNWRYADEAQVPRICFVNKLDRTGASFEHSYKT
                     ILERLSKKAVRMQIPIGLEDKHEGVVDLLKMKAYFFEGEMGNQVIEKEVPAELLEEAK
                     KYRAELVERIVEHDDAQMNAYLEGNEPPLEVLKKTLRKAVIANDIFPVFCGSALKNKG
                     VQLVLDAVVDYLPSPLDIPPMKGIHPDTEEPVERKAADSEPFSALAFKLMADPFVGQL
                     VFFRVYSGTLEAGSYVYNPRTGNKERIGRIVRMQANEREEVKKVFAGEIAAAVGLKDT
                     KTSDTLCDEASPIILERIIFPEPVISLRIEPKTKADQEKMGLALSRLVDEDPTLRVST
                     DPETTETIIAGMGELHLEIIVDRMKREFNVEANVGKPQVAYRETITAPADAEGKYIKQ
                     SGGRGNYGHVKIKIKPMDLSVDPKDLPKNTKRVESFEFVNTIKGGAIPQEYIPACEKG
                     FREGLDRGVLAGFKMVNVSVDLWDGSYHEVDSNEMAFKIAASMAVQDACKAARPVILE
                     PMMKVEVVTPDKFMGDVTGSISAKRGIIEGMEARGMNQVL"
BASE COUNT          957 a          747 c          832 g          752 t
ORIGIN      
        1 tgcttgcaat ttattcacac ctgtattacg ataacgctca caaggctttc tgcctcgttt
       61 ttatttaaag atgccaacac tcaatcagct catccgcaat aagcggtcaa aaccaaaacg
      121 caaatcaaaa gcggtggcac tttcgcgtgg ttttaatgct attaaaaaca agcccgtctt
      181 ctacccagca ccgtttaagc gcggggtgtg cgtaaaagta agtactacca ctccaaagaa
      241 gccaaactca gcaatccgaa aaatcgcccg cgttcgcctc acgaatagcc aagaagtaac
      301 ggcatatatt ccgggtattg gacataactt gcaggaacac tcggtggtaa tgatccgcgg
      361 cggtcgcgta aaagacttga tcggagtacg ctaccacatt gtgcgcggtg tgctcgacac
      421 ccagggtatc gacaagcggc gtgtatcgcg ttcgctctac ggaacaaagc gcccaaagga
      481 agctaagaaa taattaaccc gccaaaattt ttatgaaatt gttaccagaa aaaataataa
      541 ataacgttat attcctaaaa atttaggagg gtaaatcatg cgacgaccag taaaaaacag
      601 aaatatagta ggagaagata tgcgttatgg ctcggtgaag cttgagaagt ttatcaactc
      661 tattatgcaa gacggcaaaa agaacaccgc tcggacgatt gtttacaatg cccttgaggt
      721 gttgaaggaa aaagagaagg ttgaaaaccc gctcgaggtc tttgacgctg cacttaaaaa
      781 tgctggtccg cttgttgagg ttcgctcgcg ccggattggc ggcgccaact accaggtgcc
      841 ccgcgaagtc cgccctgagc gccgcactgc gcttgcgatg cgctggatta ttgagagtac
      901 gcgcaaccgt aaaggggtaa aggcagcagt ggcacttgcc gacgagctta gccaggctgc
      961 aaaaggtgaa ggaaacgccg taaaaaagaa gaaagacacc cacaaaatgg ctgaatcaaa
     1021 caaagccttt gcccacttca cgtggtaatt attttataga ctaggtttat ccccaatacc
     1081 cctctttttt gaggggtatt gactttatag aagccctggg tatacaatga agctttcata
     1141 ggataaggtt taatggttca tggtatttga ttagtacgaa ccccgacttc ggttggggtt
     1201 tttttctgcc tactctaatt tgcaaaacca ggtaatccct tatatagtat cctctacggc
     1261 tggcagcagt cttttttatt tctagtaatt atttatgaac agagactacc ccttagaaaa
     1321 agtccgaaac ttcggcatta tcgcccacat tgatgcgggc aaaacaacaa cctcagagcg
     1381 cgtgctctac tacaccggca tgagccataa aatcggcgaa gtgcacgagg gcgaaacagt
     1441 aaccgactgg atggaacaag agcgcgagcg cggcattacc attactgctg ctgcggtaac
     1501 ctgtacttgg atccctacgt acacagcggg tgataaaagc aaacagtacc ggtttaacat
     1561 tattgatact ccaggacaca tcgactttac ggttgaagta aagcgcagtc ttcgcgtact
     1621 cgacggcgcc gtggtggtgt ttgacggcgt ggcaggagta gagccgcagt ctgaaacaaa
     1681 ctggcgctat gccgatgagg cgcaggtgcc gcgcatctgc tttgtgaata agctcgaccg
     1741 taccggtgca tcgtttgagc actcatacaa aaccattctc gaacggcttt caaaaaaagc
     1801 agtgcggatg caaattccaa ttgggttgga agacaagcac gagggagtag tagatctctt
     1861 aaagatgaag gcatacttct ttgaaggcga aatggggaac caggtgatag aaaaagaagt
     1921 gcctgcagaa ttgctcgaag aggcaaaaaa ataccgcgca gaacttgttg agcgcattgt
     1981 tgagcatgat gatgctcaga tgaatgcata cctcgagggt aacgagccac cgcttgaagt
     2041 actcaaaaaa acactccgca aagcagtgat tgcaaacgat atcttcccgg tattttgcgg
     2101 cagcgcactc aaaaacaagg gtgtgcagct ggtgctcgat gcggtggttg actaccttcc
     2161 gtctccgctc gatatcccac caatgaaagg tattcaccct gacaccgaag agccggttga
     2221 acgaaaggca gctgatagtg agccattctc agctctcgca tttaaactga tggcggatcc
     2281 gttcgtcggg cagctcgtat tcttccgtgt gtactcggga acactcgagg caggctcgta
     2341 tgtgtacaat ccgcgtaccg gaaacaaaga acgtatcggt cgcattgtgc gcatgcaggc
     2401 aaatgagcgt gaggaggtga agaaagtgtt tgccggagaa attgcggcag cggtagggtt
     2461 gaaggatact aaaacctccg acacgctctg cgatgaagcg agcccaatca tacttgaacg
     2521 catcatcttc cctgaaccgg ttatttcgct ccgcattgag ccaaaaacaa aagctgacca
     2581 ggaaaaaatg ggacttgcgc tcagccgttt agttgatgaa gatccaacac ttagagtttc
     2641 aaccgaccct gaaacaacag agacgattat cgccggcatg ggagagctcc accttgaaat
     2701 cattgttgat cgcatgaagc gcgagtttaa tgttgaagca aacgtcggca aaccgcaggt
     2761 agcgtaccgc gaaacaatta ctgcacctgc tgatgctgaa ggcaaataca ttaagcagtc
     2821 gggcggtcgc ggcaactacg ggcacgtaaa aattaaaatc aaaccaatgg atctttcggt
     2881 tgatccaaag gatcttccaa aaaatacaaa gcgggtagag agctttgagt ttgtaaacac
     2941 cattaaggga ggtgcgattc cgcaggagta cattccggcg tgtgaaaaag gcttccgcga
     3001 gggcttagat cgcggtgtgc ttgccggatt taaaatggtg aacgtatctg ttgatctctg
     3061 ggacggctcg taccacgaag tcgactcgaa cgagatggcg tttaaaatcg ccgcttcgat
     3121 ggcagtgcag gatgcgtgta aagcagcacg accagttatt ttggagccga tgatgaaagt
     3181 tgaggtggta actcctgata aatttatggg tgatgttacc ggctcaattt ctgcaaaacg
     3241 cggcattatt gaaggtatgg aagcccgcgg catgaaccag gtactgaa
//
LOCUS       DATIBQ010000056         6657 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U16294
            k141_14448, whole genome shotgun sequence.
ACCESSION   DATIBQ010000056 DATIBQ010000000
VERSION     DATIBQ010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935789
            Sequence Read Archive: SRR25158331
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 6657)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6657)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:58:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 637
            CDSs (total)                      :: 603
            Genes (coding)                    :: 601
            CDSs (with protein)               :: 601
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6657
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14448"
                     /isolate="SMAG_U16294"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..363)
                     /locus_tag="VLB02_01700"
     CDS             complement(<1..363)
                     /locus_tag="VLB02_01700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56779.1"
                     /translation="MTKKILIGLGIALVIIGFFVFLYQQSNKPGKLDTFATCIKDSGA
                     RFFGAWWCPHCQNQKARFGRSAKLLPYIECSTPDRKQIENCTTEGITGYPTWKFADGS
                     VEKTEMELAELAEKTQCVL"
     gene            complement(382..789)
                     /locus_tag="VLB02_01705"
     CDS             complement(382..789)
                     /locus_tag="VLB02_01705"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HSE56780.1"
                     /translation="MKKSTNFATLLQEHALKITPLRLRILEVLDTTTVPLSIEALQKK
                     LRGKGNIVTLYRALASFEVTGIVQALTLKDATRYQLATIHQHHIICRDCGTIETIPFC
                     IEKLERGALKKSKKFKLIADHNLEFFGTCRKCT"
     gene            827..1474
                     /locus_tag="VLB02_01710"
     CDS             827..1474
                     /locus_tag="VLB02_01710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56781.1"
                     /translation="MKSFLHLHAENLRAIITGFLLVFIFFIARELVDSPFLLLEAVAV
                     MALAVFLAKKTAHHFHGHHTHAGDSPIDTVALIVLFAANILHPAVDGFSIFETFEIGG
                     TAAGIIFAGSVVLHEIFRQSALVAAFSTMHIRWGWVVGTALAGITLGVGTGFLGSEIF
                     HENERIIDLVTLFAYAFIIAEFHFSGHAKYEKRSWWFIALGITLSTALSLIAKVH"
     gene            complement(1520..1666)
                     /locus_tag="VLB02_01715"
     CDS             complement(1520..1666)
                     /locus_tag="VLB02_01715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56782.1"
                     /translation="MKIIYLFAFEPPPLALIGYVVIMSAIILLIAEWGKITKNFKLFF
                     KKKM"
     gene            complement(1878..1950)
                     /locus_tag="VLB02_01720"
     tRNA            complement(1878..1950)
                     /locus_tag="VLB02_01720"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1915..1917),aa:Lys,seq:ttt)
     gene            1999..2481
                     /locus_tag="VLB02_01725"
     CDS             1999..2481
                     /locus_tag="VLB02_01725"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HSE56783.1"
                     /translation="MKSIKSEIVLILHSIRSAHNVGSLFRTADAAGVTHIILTGYTPQ
                     PVNVFGKINKEIAKTALGAEKTIPWEHTARIGLVLKKLKAAGYTSIAIEQDARSVNYR
                     KVEARGNIAFVLGNEVTGLNTTVLTAVDVIAEIPMAGEKESLNVAAAGAVALFQILNR
                     "
     gene            complement(2478..2654)
                     /locus_tag="VLB02_01730"
     CDS             complement(2478..2654)
                     /locus_tag="VLB02_01730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56784.1"
                     /translation="MTDFKWFIVIFVFLWLVWAVTGGPARYENKTNQFIEEPSAPGDR
                     GDIYNLEQLKARQR"
     gene            complement(2658..3587)
                     /gene="mutM"
                     /locus_tag="VLB02_01735"
     CDS             complement(2658..3587)
                     /gene="mutM"
                     /locus_tag="VLB02_01735"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF002211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HSE56785.1"
                     /translation="MPELPEVETTVQGLRNTVVGLTIKDVWTDLASTDKRNTESVKNP
                     KYFTEFKKKVTGQKIVRAERRAKNILLFLKNNTVILIHMKMTGHLLYGLYAHDKKKNV
                     WIPDPKEKNEALRDPFNKFLRVVFTFTNGKHLVFSDLRKFGKITLLEEAEKIHDTKHL
                     QGIGPEPLNKLFTETLFTTQLLRRPRAKIKQALMDQSLIAGIGNIYSDEALWLAGIHP
                     LSPAAAIPKEKMKALYRAVQTVLTKGIDFGGDSMSDYRDIYGNRGTFQNAHNAYRRTG
                     KPCTKRGCSGTIARVVVGGRSAHFCGTHQKLYT"
     gene            complement(3614..4177)
                     /gene="ruvA"
                     /locus_tag="VLB02_01740"
     CDS             complement(3614..4177)
                     /gene="ruvA"
                     /locus_tag="VLB02_01740"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HSE56786.1"
                     /translation="MIALIKGIVTDKEDRGIIVDTGSIGYFVSVPEHTKEVLAVGEQV
                     TLVTHLVIRDDSHDLYGFESKPERELFLMLFSVSGVGPRTALHILSSFPLPRLISTIQ
                     NGSGKELSLVPGIGKKTGEKIVLELKEKMKLFAPEITASEVNHDVYEALVALGYKDYK
                     IHEALQGINKDISTQAQIKEALQILGK"
     gene            4235..4495
                     /gene="rpsT"
                     /locus_tag="VLB02_01745"
     CDS             4235..4495
                     /gene="rpsT"
                     /locus_tag="VLB02_01745"
                     /inference="COORDINATES: protein motif:HMM:TIGR00029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HSE56787.1"
                     /translation="MPITRSAKKALRVSDRRKSVNDRRKKVMKEEVKKVEKLVHDKKF
                     TEAKKAVAAAYSAIDKATKKGILKKNTASRKKARLSRITKEK"
     gene            4536..4796
                     /locus_tag="VLB02_01750"
     CDS             4536..4796
                     /locus_tag="VLB02_01750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56788.1"
                     /translation="MLYEFYGLECPICQGMRKLSDKLVRECGVQLERKEVWHNKENME
                     RIKQFDAQDECGGVPFYYNSETKAWLCGEVKYEELKKWAGVK"
     gene            complement(4838..>6657)
                     /locus_tag="VLB02_01755"
     CDS             complement(4838..>6657)
                     /locus_tag="VLB02_01755"
                     /inference="COORDINATES: protein motif:HMM:NF017369.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HSE56789.1"
                     /translation="DEAKTRYDFTLTDEHEKKYNLHFAKGKPGLLTGSVVKVKGTVLD
                     NELVLADGSNSSDNTSLQTVQAASVDSMGDQHTLAVMVNFRNNPTQPYTKSSIYGRIF
                     SNTDSTNVYYKESSYDLTGFTGEVTDWITIPYDGLNCSSMLDTWTNAANQAATAAGYN
                     LSNYNRHLYVLAGVYDCGWGGLSYVGGNPSRSWVVGTSNAIIDHELGHAIGMWHASSF
                     FCGTKQIDAYINCSQSEYGDLYDVMGGGSNGRRNQQNGPHKAQQGWVPQPNVQTVTTS
                     GTYTIASLETKNSAVQVLKIAKPNTSQNYYIDYRQPSGFDSTLPSTMTSGAGVLIWGT
                     SGNTMRLDSIPGDNDISNSALSDSRSFQDPINGITITQVSHTASEVTLQVTFSPSTCI
                     KAKPSMSVSPLSQTGSAGEMLRYTVSLKNNDTVGCGTNSSFDFSATGLPAGFTGTSAS
                     LFLPPGASGSVSFEVTSPTGLAEGTYLFNIVAQDYNDTVDSLHTASVQASYSAFTDTI
                     APKITITKPAAGATFTSKSKIQISASATDSAGVAKMELLFDSKVLKTCSLTTSCSVTY
                     SPSPFVAGSHVITATATDKGGNKGSVNVSITLSPGSGNR"
BASE COUNT         1678 a         1445 c         1619 g         1915 t
ORIGIN      
        1 gagtacgcac tgtgtttttt cggcgagctc ggcgagttcc atttcggttt tctctaccga
       61 gccgtcggca aacttccagg ttgggtatcc ggtaatgcct tcagtggtgc agttttcaat
      121 ttgtttgcgg tcaggagttg aacactcgat gtaggggagc agttttgctg atcgaccaaa
      181 tcgcgctttt tgattttgac agtgcgggca ccaccacgcg ccaaagaatc gtgcgcccga
      241 atctttaata catgtcgcga aggtgtcgag ctttcccggt ttatttgatt gctggtagag
      301 gaacacgaag aatccaataa tcactagtgc aatgccgagc ccaatgagaa tttttttagt
      361 catacaactg tagcctaccg tttaggtgca cttcctgcaa gtgccaaaaa attctaaatt
      421 gtggtctgct ataagcttaa atttttttga ttttttcaat gcgccgcgct ctaatttttc
      481 tatacaaaaa gggattgttt caatcgtgcc gcagtcgcgg caaatgatgt ggtgctgatg
      541 tattgttgca agctggtagc gagttgcatc ttttaacgta agtgcctgta caattcctgt
      601 tacttcaaac gaggcgagtg cccggtagag ggtaactata tttcctttac cgcgcagttt
      661 tttctggagt gcttcaatag agagtggcac tgtggtagtg tcgagtacct caagaattct
      721 tagacgcagg ggggttattt ttaacgcatg ctcttgtaaa agggtagcga aattggttga
      781 cttcttcatt ggttccagta tactcaaacg caactaattt gcaattatga aatcttttct
      841 tcacctccat gctgaaaacc ttcgtgcgat aattaccggc tttttgctgg tatttatttt
      901 ttttattgca cgagaacttg tcgattcccc gttcttgctt ttggaggcag ttgcagtaat
      961 ggcacttgca gtgtttcttg caaaaaaaac tgcgcatcac tttcatggac atcacacaca
     1021 tgcgggcgat tcgccaatcg atactgttgc gctcatcgtg ctgtttgcag caaatatcct
     1081 acatccggca gtcgatggtt tttcgatatt tgaaacattt gaaatcggcg gtactgctgc
     1141 gggtattatc tttgccggaa gtgttgtgct ccacgaaatt tttcgccaat cggcgcttgt
     1201 tgctgcgttt tctaccatgc acattcgctg gggctgggtg gttggcacgg cacttgcagg
     1261 tatcacgctt ggtgttggta ccggtttcct tggttcagag atttttcacg aaaacgagcg
     1321 gataattgat ttagtgacac tctttgccta tgcgtttatc attgctgagt tccattttag
     1381 cggtcacgcg aagtatgaaa agcgctcatg gtggtttatt gcacttggta ttacacttag
     1441 taccgctcta tcacttattg caaaagtgca ctaagacata aaaatgccga acctgttaag
     1501 gttcggcatt ttagttttct tacattttct ttttgaaaaa tagtttaaag ttttttgtaa
     1561 tttttcccca ctcagcgata aggaggatga ttgctgacat aatgaccacg tacccaataa
     1621 gcgctagtgg aggtggctca aaggcaaaaa ggtatattat tttcatgatt ggttttttga
     1681 taacggtaca aaaaaaatgg gtggagtgtc aatgttgtgc gtccagcagg aatttctccc
     1741 gcgactaaat ttgctgcagc aaatttgtct gggcaccgcc tcgcttgtct gcctgctggc
     1801 agacatagct ccggcgttcg aatcctgcac gcgagcaacg caggttgctc gcttagacgc
     1861 acaatacttc gtatttgtgc gtccagcagg attcgaacct gcgaccgtct ccttaaaagg
     1921 gagctgctct accgactgag ctatggacgc gttttaaaca cttgaaaaaa tgccgaaaaa
     1981 ctataccatg caagcactgt gaaatcaata aaatcagaaa tcgtgctgat actccatagt
     2041 atcagaagtg cgcacaatgt gggctcgctc tttcgcactg ccgatgcggc aggggtaaca
     2101 catattatac tcaccggcta tacaccgcag ccagttaatg tatttggcaa aataaataaa
     2161 gaaatcgcta agacggcact cggggcagag aaaacaattc cttgggagca cactgcccgt
     2221 attggactag tacttaaaaa actaaaagcg gctggatata cgagtattgc aatcgagcaa
     2281 gatgcgcgtt cagtgaatta tcggaaggtg gaagcaaggg ggaatattgc attcgtgctc
     2341 gggaatgaag tgacaggact caataccaca gtacttaccg cagtagatgt tattgctgaa
     2401 attccgatgg ctggcgaaaa ggagtcgtta aatgttgctg ctgccggagc agtagcgcta
     2461 tttcaaatac ttaatcgcta acgctgccgg gcttttagtt gttctaggtt gtagatatcg
     2521 ccacgatcgc ccggagcaga aggttcttca ataaattggt ttgtcttgtt ttcatagcgc
     2581 gccggaccgc cggtaactgc ccacaccagc cacagaaaaa caaatataac gataaaccac
     2641 ttgaagtcgg tcataggcta agtatagagc ttttggtgtg tgccgcaaaa gtgtgcactt
     2701 cgcccgccga caacaactcg cgcaatggtc ccgctacagc cgcgtttagt gcatggcttt
     2761 ccggtgcggc ggtacgcgtt gtgggcattc tggaatgttc cgcggttgcc ataaatatcg
     2821 cggtagtcgc tcatcgagtc tccaccaaag tcgatacctt ttgtaagtac ggtttgcact
     2881 gctctataca gtgctttcat tttttctttt gggatagctg cagcaggcga gagtgggtgg
     2941 atgccagcca accagagtgc ttcatcagaa taaatatttc caataccggc aatgagcgat
     3001 tgatccatga gcgcttgttt tatttttgct cgtggtcggc gcaggagctg agtagtaaat
     3061 agggtttcag taaataattt gttgagcggt tcgggaccaa taccttgcaa atgttttgtg
     3121 tcatgtattt tttctgcctc ctcgaggagg gtgattttcc caaatttacg caagtcgcta
     3181 aaaaccaggt gcttgccgtt ggtgaaggta aacacgacgc gcaaaaattt gttgaatggg
     3241 tcacggagcg cttcgttttt ttcttttggg tcaggaatcc agacattctt ctttttatcg
     3301 tgtgcataca acccgtagag caggtgacca gtcatcttca tatgaatgag aatgacggtg
     3361 ttgtttttta agaaaagcaa aatatttttt gctcgtcgct cggcacgtac gattttttgc
     3421 ccggttactt ttttcttaaa ctctgtaaag tactttggat ttttaacact ttcagtgttg
     3481 cgcttatcag tagaggcgag atcggtccat acgtctttaa tcgtgagccc aaccacagtg
     3541 ttacggagcc cttggacggt agtttcaact tctggtaatt ctggcataca tttatcctga
     3601 gcttgccgaa ggattatttc cctaaaatct gcaatgcttc ttttatttgc gcctgtgtag
     3661 atatatcctt attgataccc tgcagcgctt catggatttt ataatcctta tagcccagag
     3721 caacgagcgc ttcgtacaca tcgtggttca cctcgctcgc ggtgatctcc ggagcaaaaa
     3781 gtttcatctt ctctttcagt tcaagaacga ttttttctcc cgtttttttg ccaatgccgg
     3841 ggacgagcga gagctctttg ccgctgccat tctgtatggt gctgatgagc cgggggaggg
     3901 ggaatgaact taaaatgtgg agtgcagtgc gcggaccaac acccgaaact gagaagagca
     3961 tcaaaaacaa ttctcgctca ggttttgatt caaaaccgta caggtcgtgc gagtcatcgc
     4021 ggataacgag gtgggttacc agggtaactt gctcaccaac agccagcact tctttggtgt
     4081 gctcggggac tgaaacaaag tagccaatgc tgccggtgtc gacaatgatg ccacggtctt
     4141 ctttatcggt cacgataccc ttaataagcg caatcatggg caaactatac caaatattgc
     4201 agattttggg catgtttggt atagtttgcc cactatgcca atcaccagat cagcaaaaaa
     4261 agcgctccgg gtttcggacc gccgcaaatc agtcaacgac cgccgcaaga aggttatgaa
     4321 agaagaggta aaaaaggtag aaaaacttgt ccacgataag aagttcaccg aagcaaagaa
     4381 ggcagttgct gcagcgtaca gtgctatcga caaagctacc aagaagggaa ttttgaagaa
     4441 aaatactgcc agccgcaaaa aggctcgtct ctcccgaatc accaaagaaa agtagcaaaa
     4501 acccgccagt tggcggtttt tgttatactc tgcagatgtt atacgaattc tatgggcttg
     4561 agtgcccgat ttgccaaggg atgcgaaagc tctctgataa gcttgtaaga gaatgcggcg
     4621 tacagttaga gcgtaaagaa gtgtggcaca acaaagaaaa catggagcgc atcaaacagt
     4681 ttgatgcaca agacgagtgc ggtggcgtac ccttctacta caatagcgaa acaaaagcct
     4741 ggctctgcgg ggaagtgaaa tacgaggagc tcaaaaagtg ggcgggggta aaataaaaaa
     4801 ccggcatata ccgccggttt ttttatttaa gctagtatta gcggtttccg ctacctggac
     4861 ttagggtaat tgaaacgttc actgatcctt tgttgccacc cttgtcagtt gcggttgcag
     4921 taataacgtg actgccggct acaaaaggag atgggctata ggtaactgag catgaggtag
     4981 ttaagctgca tgttttaagt accttgctat cgaagagaag ctccattttt gcaacacctg
     5041 cactgtctgt tgccgaagca gaaatctgaa ttttactctt actggtaaat gttgcgcccg
     5101 cagctggttt ggtaatagtg atttttggag caattgtgtc agtgaaggca ctgtaacttg
     5161 cttggacact agcagtatgg agagagtcca cagtatcgtt gtagtcttgt gcaacgatat
     5221 tgaaaaggta ggtaccctca gctaggccag tcggagaggt gacctcaaac gatactgaac
     5281 cgcttgcccc tggaggtaag aataatgaag cactagttcc agtaaagccg gccggtagcc
     5341 ctgttgcaga gaaatcaaaa ctgctgtttg ttccgcaccc tactgtatcg ttattcttta
     5401 gtgaaactgt atacctgagc atttccccag cgctgccggt ttggctgaga ggagaaacac
     5461 tcattgatgg ttttgctttt atgcaggtgc tgggactgaa ggtcacctgg agtgttactt
     5521 cacttgcggt gtggctcacc tgggtaatag tgatgccgtt aataggatcc tggaaagagc
     5581 ggctgtcgga aagagcagaa tttgaaatat cattgtctcc cggaatcgaa tcgaggcgca
     5641 ttgtattgcc acttgttccc cagataagta cgcctgctcc tgaagtcatt gttgacggca
     5701 aagtgctatc aaagccggat ggttggcggt agtcgatata gtaattctga gaagtgttcg
     5761 gtttcgcgat tttcaaaact tggacggcac tgttttttgt ttcaagagat gcgatagtat
     5821 atgtaccgct tgtggtaact gtttggacat ttggctgtgg tacccatccc tgttgagcct
     5881 tatgcggtcc attttgttgg tttcttctgc cattactacc accacccatt acgtcataga
     5941 gatccccgta ttcgctctgg ctgcagttta tatatgcatc gatctgtttt gtgccacaga
     6001 aaaaacttga tgcatgccac attccgatcg cgtgcccaag ttcatgatcg atgattgcat
     6061 tactcgtgcc gaccacccat gatcggcttg ggttgccgcc aacgtacgaa agtccgcccc
     6121 agccacaatc gtagactccg gcgagtacat ataggtgccg attgtagttg gaaagattgt
     6181 accctgcggc agtagcggct tggttagcag cattagtcca cgtgtccaac atggaactgc
     6241 aattcagccc atcgtagggg atagtgatcc agtcggtaac ctctccagtg aaaccggtaa
     6301 gatcatacga gctttccttg taatagacgt ttgtcgaatc ggtgttgctg aaaatccttc
     6361 cgtatatgct tgattttgtg tatggttggg ttggattatt cctaaagttt accatcacag
     6421 ccaaggtgtg ctgatcaccc atcgagtcga cacttgctgc ttgaactgtt tgcaagctgg
     6481 tattatctga actattcgaa ccatctgcga gcacaagttc attgtcgagg acagtacctt
     6541 tcactttcac gaccgagccg gtcaatagtc ctggttttcc tttggcaaag tgcaagttgt
     6601 atttcttctc gtgctcatca gtgagagtga aatcatacct agtttttgcc tcatctt
//
LOCUS       DATIBQ010000057         6815 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U16294
            k141_1849832, whole genome shotgun sequence.
ACCESSION   DATIBQ010000057 DATIBQ010000000
VERSION     DATIBQ010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935789
            Sequence Read Archive: SRR25158331
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 6815)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6815)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:58:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 637
            CDSs (total)                      :: 603
            Genes (coding)                    :: 601
            CDSs (with protein)               :: 601
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6815
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1849832"
                     /isolate="SMAG_U16294"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..779
                     /locus_tag="VLB02_01760"
     CDS             <1..779
                     /locus_tag="VLB02_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HSE56790.1"
                     /translation="IATNMAGRGVDIILGGTPYDEQLHEEVKSLGGLFVIGTERHEAR
                     RIDNQLRGRSGRQGDPGETQFYVSLEDDLMRIFGSGRIKSMMGRFGIPEDQPIQSGLI
                     SRALESAQQKIEGLNFDARKHTLEYDNVIAHQRSVVYGRRRALLLGDIPHVQSVYYEL
                     GEREALERLANEKSNLVGEVQFWDMVRRIILYTTDTLWIDHIDTMEHLRQSVGLRAYG
                     QREPLVEYKKEALIRYRDMEFSLKEQVLVLVEGIGKQAKK"
     gene            1063..1623
                     /locus_tag="VLB02_01765"
     CDS             1063..1623
                     /locus_tag="VLB02_01765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56791.1"
                     /translation="MHTQLKQVFKAFILVPFLATSMSITTFNTAVDTAVANQMQDKAA
                     LQAQADREQKAKKIDTYFAKFNLPIAGYGNAMVLAGEKYGIDPYLIAGLAMRESTGCK
                     FIIPHTHNCFGWGGGKIKFDSFDEAIEVIAKNLGGHNEKTAHYYKGKNTLKILETYNP
                     RSVVPTYPEEVIGIMSKIENTDITSA"
     gene            complement(1671..2072)
                     /locus_tag="VLB02_01770"
     CDS             complement(1671..2072)
                     /locus_tag="VLB02_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011692895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HSE56792.1"
                     /translation="MDKVQHFEIPSDDVARSRAFYETAFGWQTKEWPMPDGSTYIGLY
                     TGPVDEKNMWKEPGFINGGMFKRGESFPVTNQTIAIVVQDIDASLAKVTAAGGSVLME
                     KKEIGGMGYYAYIQDTEGNTIGVWQDIKKEM"
     gene            2216..3334
                     /locus_tag="VLB02_01775"
     CDS             2216..3334
                     /locus_tag="VLB02_01775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56793.1"
                     /translation="MKKQLVLLAMLTTLFISCKKEKDGPKNPTFQKDEGFTNRTIASG
                     MNVLIAKYILTVGAMDGFIFIPDFYVAGSTVVPFKNFYFTVKRSDSSLVYKAPARTIS
                     QYYVDSHILLEKDARYSVAVYADISPGSIGMVNVQCQTRYEWVGGTGVLEKLAGQETM
                     VTPYKSTPTLTHIPLTGLIVDSVEAKQYEFSIAGKTAHKQFAFAVQVADNGANDTLSL
                     SKLKCFEDGVDVTSKVTFMDGNGTPISVITENTGKIFVTYTAGSGETVVDGTRIFSLK
                     ATPSGFHHPNDGDGYSLQLITDQNAVPDGYWYLNRGSSGSHAKLSSTATASGSAIAGN
                     LIWSDMSAGSHSALFGISSNDWANGYKIPALPMQIWHQ"
     gene            3448..3897
                     /locus_tag="VLB02_01780"
     CDS             3448..3897
                     /locus_tag="VLB02_01780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56794.1"
                     /translation="MIKQIIGSQFPGSMIIDTNITEVHALINQQQEQRMFFRSIQKIL
                     FPVEKNPLESWLATNPDTMQIFLRGNGVSITTNDLVAITMLEIAIFEPGSQEVFGIIY
                     GSENEISYHKKKDGDKDFGDIDDIPVHFKHLGKLLVKVLHENGVILS"
     gene            complement(3967..4575)
                     /locus_tag="VLB02_01785"
     CDS             complement(3967..4575)
                     /locus_tag="VLB02_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56795.1"
                     /translation="MFTLTKEETAIFKQLKTPAKIQDFLNSLPFNFELKGETCRSPRR
                     VLQHKTAHCLEGALFAAAALWFHGHKPLLLDLKSTKKDFDHVVALFKKDGCWGAISKT
                     NHAVLRYREPIYKTVRELALSYFHEYFLDDGSKTLRSFSRPLSLKQFGTSWITAEEDL
                     WDIGATLDDAPHSPILTPSAIKNLRKADKVEIEAGKITEYKK"
     gene            complement(4640..4936)
                     /locus_tag="VLB02_01790"
     CDS             complement(4640..4936)
                     /locus_tag="VLB02_01790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56796.1"
                     /translation="MGIEIQAKFEKSKKLVEDFEKKLRAGEKILTTGTGKMSLLLRAK
                     NSFSTGNYASGIDFLKEALDAKTLPSNSEFITFFKGKLQALAAEFRYKPYDKAA"
     gene            complement(4997..>6815)
                     /gene="dnaE"
                     /locus_tag="VLB02_01795"
     CDS             complement(4997..>6815)
                     /gene="dnaE"
                     /locus_tag="VLB02_01795"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HSE56797.1"
                     /translation="SQGFPMTIDHAIDIIPELKELYDTDRGTKEIIDMAKKIEGNVRH
                     ISVHAAGVIISPTPDITEFTPIQFDPKGEGKIITQYDMFSGGRDGVVNLPKFDMLGIR
                     NLQFISGTIDRVKKIRSIDIDIDTIPLDDKKVFEILSRGETVGVFQMAGTGMTQYLKE
                     LKPEKVEDLMAMVALYRPGPMEVIPEYIKRKQNPRLISYPDPRLKDDLEASYGLLVYQ
                     DDVLITAIRLAGYSWLDADKFRKAMGKKIPAEMAEQKDKFYKGCTEYGGLTKKHIDEL
                     WKKIEPFAAYGFNKAHAASYGMVAYKTAYLKANYPAEYMTACMTAESGDIETCSEYIE
                     EARRMGFAILPPDINESFSDFTVVVEDGVVTKKIRFGLNNIKNFGTEIGKAIIAERKA
                     RGKFESLESFLERVTHKNLNKKSVEALISCGALDAFGDRGVLFANIESLLSFHKGITS
                     TANASQDSLFEGLASKPKAVLALKPTEPAPLTQKLAWEKELLGLYISGHPLDEHQDKI
                     EKSGNTIKTLLGKGECKGKILVAAVHDVRVLYTKNNSKMAFVVLQDKTGTLEGVVFPE
                     AFKTIEPVLQPGKVIAIRCNLAKRNEELSLVIDQAKELG"
BASE COUNT         1816 a         1430 c         1539 g         2030 t
ORIGIN      
        1 tgattgcgac aaacatggca gggcgcggcg tcgacattat tttgggcggc acaccctacg
       61 atgagcagct gcatgaagag gtgaaatcgc tcggcgggct ttttgtaatt ggtaccgagc
      121 ggcatgaggc acgccggatt gataaccagc tccgcggtcg cagtggtcgg cagggcgatc
      181 cgggcgaaac ccagttttat gtgtcgctcg aagatgatct catgcgcatt ttcggcagcg
      241 gacgcattaa aagtatgatg gggcgctttg gtattcctga agatcagcct attcagagcg
      301 ggcttattag ccgcgcgctt gaatctgctc aacagaaaat tgaagggctt aactttgatg
      361 cccgcaagca cacactcgag tacgataacg tgattgcgca ccagcgcagt gtcgtgtacg
      421 gtcgccgtcg ggcgctcctc ttgggagaca tcccgcatgt gcaaagtgtg tactacgagc
      481 tcggtgagcg tgaggcactc gaacggctcg ctaatgaaaa gagtaacctt gttggcgaag
      541 tgcagttttg ggatatggtt cgccgaatta ttctctatac aaccgatacg ctctggattg
      601 accacatcga taccatggag caccttcgcc agtcggtggg gcttcgtgcg tatggccagc
      661 gagagccatt agttgagtac aaaaaagagg cactgattcg gtaccgagac atggaatttt
      721 cgctcaaaga gcaggtgttg gtgctcgtgg aggggatagg gaagcaagcg aagaagtaac
      781 tatttttgct atctaaaata tactgatttt tgaatcaaaa ttaaacacta tttttgacac
      841 tctgtaaaga aatgtactat aaaataacct tataaaataa gggtattttt agtttgacaa
      901 agaaataaat cattgcataa aattgacaaa tgggtaagga tgtgctacta tgtacgcagt
      961 tgcttatcga ggtttttggt gagcaatttt catttggttt ctgaaaagaa agcgatggaa
     1021 gacactcgtt actgaagttc ccccggagct caaccgggac ttatgcatac acaattaaaa
     1081 caggttttta aggcattcat tcttgttccg tttcttgcta ccagtatgtc aattaccacc
     1141 tttaacacgg cagttgacac cgcagtagca aaccagatgc aggacaaagc ggcgctccag
     1201 gctcaggcag atcgagagca aaaagcaaag aaaatcgata cgtactttgc aaaattcaat
     1261 cttccgatcg ccgggtatgg taacgcgatg gtgcttgctg gtgaaaaata cggcattgac
     1321 ccgtacctta ttgcaggttt ggccatgcgt gagtctaccg ggtgtaaatt cattatcccc
     1381 catacacaca actgttttgg atggggtggc gggaagatca agttcgatag tttcgatgag
     1441 gctatcgaag taatcgccaa gaaccttggt ggtcacaatg aaaagactgc tcactattac
     1501 aaagggaaaa ataccctcaa aattcttgaa acctataacc cccgttcagt ggtgccaacg
     1561 taccctgaag aggtcatcgg aatcatgagt aaaattgaaa acactgacat tacgtcggcg
     1621 taaatagata accaaaacca ccactcgaga gagtggtggt tttgtatact ttacatttcc
     1681 tttttaatat cttgccacac cccaatagtg tttccttcgg tatcttgtat gtaggcgtag
     1741 tagcccatac cgccaatttc tttcttttcc ataagaactg aacctcctgc agcagtaact
     1801 tttgcaagtg atgcatcaat gtcttgtact acaatcgcaa tagtttgatt agtcaccggg
     1861 aaactttcac ctcgcttaaa catccctccg ttaataaacc ccggctcctt ccacatattc
     1921 ttttcatcaa ctggtccagt gtagagacca atgtaggttg agccgtcagg catcggccac
     1981 tcttttgttt gccagccaaa tgctgtctca taaaatgctc gactgcgtgc tacatcatca
     2041 cttggaattt caaaatgttg tactttgtcc atagtttttt ctcgtttaaa aatcagtaat
     2101 ttatcaattt taacacccgc tcatactgcg gtatctattg actttttata tataatgttc
     2161 tattataaaa ccagaatttt ggacattgaa aatcaaaact taaaactaat aagaaatgaa
     2221 aaaacagctt gtactattag caatgcttac tactttattc atatcctgta aaaaggaaaa
     2281 ggatggacca aaaaacccaa ccttccaaaa agacgaaggt tttacaaacc ggactattgc
     2341 ttccggaatg aacgtactca tagcaaagta cattcttaca gtaggagcta tggacgggtt
     2401 tatctttatt cccgatttct acgttgcggg aagcactgtt gttccgttca agaatttcta
     2461 ttttacggtc aaacggagcg attcttcgct ggtgtataaa gcgcccgcaa gaactatttc
     2521 ccaatattat gtagattccc atattctttt agaaaaggat gcccgatact cggttgcagt
     2581 atatgccgat atttctccgg gaagtattgg tatggtgaac gttcagtgcc aaaccagata
     2641 tgaatgggtg gggggaaccg gcgtgcttga aaagcttgca ggtcaggaaa ctatggtaac
     2701 cccctataag tctactccca ctctcaccca tatcccctta acgggattga ttgttgatag
     2761 tgtagaggcg aaacagtatg aattcagtat tgccggaaaa acagctcata agcagtttgc
     2821 gtttgcagta caggttgcag ataatggcgc taacgacacc ctctcgctgt cgaagcttaa
     2881 gtgcttcgag gacggggtag acgtaacgag caaggttact tttatggatg gaaatggtac
     2941 tccaatttct gtaatcactg agaatacagg aaagatcttt gttacctaca ctgctggttc
     3001 aggagaaacg gtggttgatg ggacaagaat attttcccta aaagcaacgc cgagtgggtt
     3061 ccatcacccg aatgacggtg atgggtacag cctgcagttg attactgacc agaacgctgt
     3121 tcctgatggg tactggtact taaaccgggg atcctcagga agtcatgcaa agctcagtag
     3181 cactgctacc gctagcggaa gcgcgatagc ggggaactta atatggtctg atatgagtgc
     3241 cggctcgcat tcggctctct tcggaatttc gtccaatgat tgggcaaatg gatataaaat
     3301 tccagccttg ccgatgcaaa tttggcatca gtagtaatat tttttcattt acaaaacccc
     3361 cacttttaaa gtgagggtta ttttttttat caaatttata tttgacataa ttaaatttat
     3421 gtgatataaa taaaaaaaag aaaaatcatg attaagcaaa ttatcggaag tcagtttccg
     3481 ggttctatga ttatagacac aaacataaca gaagttcatg ctttaataaa tcaacagcag
     3541 gaacagcgta tgttttttag atcaattcag aaaattttat ttcctgtaga aaaaaacccc
     3601 ctcgaaagtt ggttagctac taatcctgat actatgcaaa tctttctcag aggaaatgga
     3661 gtatctataa ccactaatga tcttgttgcg ataacaatgt tggaaatagc gatttttgaa
     3721 ccaggaagtc aggaagtttt tggaatcata tatggttctg aaaatgaaat ttcttaccac
     3781 aaaaagaaag atggagataa agattttggc gatattgatg atattcctgt tcattttaaa
     3841 catttaggta agctattagt gaaagttctt catgaaaatg gagttattct ttcatagtta
     3901 gtttttcaag actgctacta tacattggta gcagtctttt atgtttgtat gaaagtactt
     3961 ttaaatttac tttttatact cagtaatttt tccagcctca atttctacct tgtctgcttt
     4021 gcgcaggttt ttgatggcac tgggcgtgag gatgggtgag tgcggcgcat catcgagtgt
     4081 tgcgccaatg tcccacaaat cttcctcggc ggttatccag ctcgtaccaa attgcttgag
     4141 gctcaacggt cttgaaaaac tacgcaatgt tttactgccg tcatctaaaa agtattcatg
     4201 aaagtacgag agggcaagct cgcgaactgt tttatagatc ggctcccgat agcgcaagac
     4261 ggcgtggttt gttttactga tcgcgcccca acaaccgtct tttttgaaga gtgctactac
     4321 atgatcaaaa tctttttttg tactcttcaa atcaagtagg agaggtttgt gcccatggaa
     4381 ccagagtgcc gctgcggcaa acagcgcgcc ctctaaacag tgcgcggttt tatgttggag
     4441 cactcgccgc ggcgacctgc aggtctctcc ttttagttca aagttgaagg gaagggaatt
     4501 gagaaaatct tggatttttg ccggagtttt tagctgtttg aaaatcgcag tttcttcttt
     4561 agtgagtgta aacaccaaaa aagtatagca ctagagataa aaaagatccg gaaatgattc
     4621 cgaatcttta aagccagtat tacgctgctt tatcgtaagg tttgtaacga aattcagcag
     4681 cgagagcttg aagttttccc ttaaagaaag taatgaattc actgttgctg gggagtgttt
     4741 ttgcatctag tgcttctttt aagaaatcga ttccacttgc gtaatttcct gtagagaagc
     4801 tattttttgc tcttagaagc aggctcattt ttccagtacc ggttgttaaa attttctctc
     4861 cagctcttag ttttttctca aagtcttcaa caagtttttt tgacttttca aattttgctt
     4921 ggatttctat tcccatgtgt ataggagttt gtttgattcc gattgtagca ctgtctacct
     4981 aatatgcaac tcggacctac cccagttctt ttgcctgatc aattacgagc gaaagttctt
     5041 cgttacgctt tgctaaatta cagcgaatag cgattacttt gccgggctga agtaccggct
     5101 caattgtttt aaatgcttcc ggaaatacta ccccttcaag tgtccctgtt ttatcttgga
     5161 gtaccacgaa tgccattttg ctgttatttt ttgtgtagag cacgcgaaca tcgtgaaccg
     5221 ctgccactaa aatctttcct ttacattcgc cttttccgag cagtgttttg atggtgttcc
     5281 ctgatttttc aattttatcc tggtgctcat cgagcgggtg gccagaaatg tacagcccaa
     5341 gtagttcctt ttcccaggcg agtttttggg tcagtggtgc tggctcagtt ggttttaatg
     5401 cgagtaccgc ttttggtttt gaggcgagcc cctcaaataa agagtcttgg ctcgcatttg
     5461 cggtactcgt aatgcctttg tggaacgaga gcaaggattc gatatttgca aacagtacgc
     5521 cgcgatcacc aaaggcatcg agggcgccgc agctaatgag cgcttcaact gattttttgt
     5581 ttaaattttt atgagttacg cgctcgagaa atgattcgag actctcgaat tttccacgcg
     5641 cttttcgttc agcaattatt gctttgccga tttcagtacc aaagttttta atgttattga
     5701 gtccaaagcg aatttttttt gttaccacac cgtcttcaac tactaccgta aagtctgaaa
     5761 atgattcgtt aatatcgggc ggcagaatcg caaaccccat ccggcgggct tcttcaatgt
     5821 attcactgca ggtttcgata tcgccggatt ctgccgtcat gcaggcggtc atgtactcgg
     5881 cagggtagtt tgctttgagg tacgctgttt tgtaggcgac catgccgtag cttgctgcgt
     5941 gcgctttgtt aaatccgtag gcggcgaaag gttcaatttt tttccagagt tcgtcaatat
     6001 gtttcttggt gagtccgccg tactcggtac agcctttgta aaatttatct ttttgctcag
     6061 ccatttctgc cggaattttt ttacccatgg ctttacggaa tttgtcggca tcgagccacg
     6121 agtagcccgc caggcgaatg gcagtgatca gcacgtcgtc ttgatacacc aaaagcccat
     6181 acgatgcttc aaggtcgtct ttaagccgcg gatcagggta cgaaataagc cgcgggttct
     6241 gcttgcgctt aatgtactcc ggaattactt ccattggtcc ggggcggtag agcgcgacca
     6301 tcgccatcaa atcttctact ttttctggtt tcagttcttt taagtactgg gtcatgccgg
     6361 tacctgccat ttggaacaca cccacggttt cgccacggga aagtatctca aacacttttt
     6421 tatcgtcgag cggaatggta tcgatatcaa tatcaatgct gcggattttt ttgacacggt
     6481 cgatagtgcc ggaaataaac tgcaggttcc tgatccccag catgtcgaac tttggcagat
     6541 ttaccacgcc gtcgcgtccg cccgagaaca tgtcgtactg ggtaataatt tttccttcac
     6601 ctttcggatc aaactgaata ggggtaaact cggtgatgtc gggggtagga gagataatta
     6661 ccccggcggc gtgcaccgaa atgtgccgga cgtttccttc aatctttttt gccatgtcga
     6721 taatttcttt ggtgccgcgg tcagtgtcgt agagctcttt tagttcggga ataatatcga
     6781 tggcgtggtc gatggtcatg gggaaccctt ggctt
//
LOCUS       DATIBQ010000058         3476 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U16294
            k141_2474753, whole genome shotgun sequence.
ACCESSION   DATIBQ010000058 DATIBQ010000000
VERSION     DATIBQ010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935789
            Sequence Read Archive: SRR25158331
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3476)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:58:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 637
            CDSs (total)                      :: 603
            Genes (coding)                    :: 601
            CDSs (with protein)               :: 601
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3476
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2474753"
                     /isolate="SMAG_U16294"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            150..812
                     /locus_tag="VLB02_01800"
     CDS             150..812
                     /locus_tag="VLB02_01800"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HSE56798.1"
                     /translation="MKKQLLLSVLGVFLLGGSVFHSAQAQACLTIIEIKITPAVTGQP
                     NHWDIYIKWNKPTPSEAERLVLTANAVGLGGGIAQTVYMSGCINLLASTDSSFTAPSI
                     PGLTKPTVKFYAYLNNCAVENDTCNTQILSPSGERRGTSKAAIEQHGNRVFIYDLSPT
                     NTAVSLRNMSGNIVQTVQATQLNRKVAEFSINNLKVGIYILTYQNKYGKNVSEKIIRQ
                     FF"
     gene            891..2816
                     /locus_tag="VLB02_01805"
     CDS             891..2816
                     /locus_tag="VLB02_01805"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HSE56799.1"
                     /translation="MEQNTFLEGLNDAQRSAVLATDGPVLIVAGAGAGKTKTVVHRIC
                     HLIVSGVAPSQILAVTFTNKAAAEMRERVGVMVEKIPGVTRGEALPFVSTFHSFGVFL
                     LRKFGAHNGQLKRFTMLDDADTMALIKESLAYHSIDPKQYEPRAIRSLISKSVCQFLQ
                     PSDIANEPNPYYKIAARVWERYEHLKREQKALDFDDLLTHTVLLLEKNKEALDWCHNT
                     WSHIHVDEYQDTNEPQYRIATLLAQASRNLCVVGDSDQSIYSWRGANIKNILEFERDY
                     PDATVILLEENYRSTKTIIQSANEIIAKNKYRKPKNLFTNNQDGESISLYCAYDEADE
                     ARFIVERAIEQLDKGVRAEEIAVLYRTNFQSRVLEEAFLNAHIPYHVLGVRFFERKEI
                     KDAVSYLRAALTPESTLDIKRTINTPARGIGKTTLLKVFSGDREALPAKAKAAVAAYY
                     ALLDSVRAFAEAHPVSHTLKYIITESGLEKHYQAEPDGEEHLENLRELVTLSLKYDHL
                     PGIAGAEKLIEDATLASDQDSLESKDEKKKGVKLMTIHASKGLEFNVVFVAGLEHGLF
                     PHERDAKQAGPDGEEERRLMYVALTRARQKLFLSYASMRTIFGMRDVRLPSEFLLDIP
                     EELITRETRDGEFTVYL"
     gene            complement(2853..3254)
                     /locus_tag="VLB02_01810"
     CDS             complement(2853..3254)
                     /locus_tag="VLB02_01810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56800.1"
                     /translation="MPPLQQPHPKKIIKYILLVLLGVLLVAAIILTFQVQRIKAFGGN
                     FYAGRRTGSLSCTCTNNTLIYIQDVVSNTSLALIYDSSTKLYANNNIYGTYLMGSYSS
                     GGQCEQYVGEECEEVKSDGTMDSNPGTGTSF"
     gene            3332..>3476
                     /locus_tag="VLB02_01815"
     CDS             3332..>3476
                     /locus_tag="VLB02_01815"
                     /inference="COORDINATES: protein motif:HMM:NF012616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA adenine N-6-methyltransferase family
                     protein"
                     /protein_id="HSE56801.1"
                     /translation="MHAKKRFGQHFLKSAAALAHMVAASALTEKDTVLEIGPGTGILT
                     EKLL"
BASE COUNT         1018 a          784 c          801 g          873 t
ORIGIN      
        1 tacttgcaag tacatttgta aaatattaat tttttccttt tccttttacc ccgccgtctc
       61 gaactcgagg cggctttttt gttgactagt tgacaatatt caaaatcagg gtacatttga
      121 atcaaacaaa ctctttaaaa ctttttatta tgaaaaaaca attacttctc tcggtattgg
      181 gtgtttttct attaggtgga agtgtttttc attctgctca agcccaagca tgtcttacca
      241 ttatagaaat aaaaatcaca ccggctgtta cagggcaacc caatcattgg gatatttaca
      301 ttaagtggaa caaaccaaca ccctcagagg cggagcggct tgtgctgaca gccaacgctg
      361 tcggcttagg tgggggtata gcgcagacag tgtatatgag tgggtgtatt aacctactcg
      421 cttctactga tagctcgttt accgcaccgt caatcccggg gcttaccaaa ccaaccgtca
      481 aattctacgc gtaccttaat aattgcgctg tagaaaatga cacctgcaac acccaaattc
      541 tttctccttc aggagaaaga aggggaacaa gcaaagctgc tatagaacaa cacgggaacc
      601 gtgtgtttat ctacgattta tcaccaacaa acacggcagt ctcattaaga aatatgtctg
      661 ggaatattgt gcaaaccgta caggcaacac aattaaacag aaaggtagca gaattttcaa
      721 ttaacaatct gaaggttgga atctacattc ttacctatca gaataagtat ggaaagaatg
      781 tttctgaaaa aattatacgg cagttttttt gattgttttt gtttggtatt agccacctca
      841 aactcgaggt ggctttttgt tttctctaac gcacggtaca ctcttcccac atggagcaaa
      901 atacttttct tgaagggtta aacgatgcgc aacgcagcgc ggtgctggca actgatggac
      961 cggtactaat tgttgccggc gcgggtgctg gaaaaacaaa aacagtggtg catcgcattt
     1021 gtcatttaat tgtaagcggt gttgcgccat cacaaatttt agcagttaca tttaccaaca
     1081 aagcggcagc cgagatgcgc gagcgagtgg gagtaatggt tgaaaaaatt ccgggagtaa
     1141 cccgcgggga agcattgccc tttgtttcaa catttcacag ttttggtgta tttttgctcc
     1201 gcaagtttgg cgcgcataac ggacaattaa aacgattcac catgctcgac gatgccgaca
     1261 ctatggcact cattaaagaa tcgctcgcgt accactcgat tgatccaaaa caatatgagc
     1321 cgcgggcgat cagaagttta atcagcaaat cagtctgcca atttttacag ccgagcgata
     1381 ttgcaaacga gccaaatcca tactacaaaa ttgcggcgcg ggtatgggag cgctacgagc
     1441 acctcaagcg tgaacagaaa gcgctcgact ttgacgacct cctcacacac acggtactgc
     1501 tgttagaaaa aaacaaagaa gcactcgact ggtgccacaa tacgtggagc cacattcatg
     1561 tagatgagta tcaagacacc aacgaaccac agtatcggat tgccacccta ctcgcgcaag
     1621 cctcgcgcaa cttgtgcgtg gtgggcgaca gcgaccagag catttactcg tggcgcgggg
     1681 cgaacataaa aaatatttta gagttcgagc gcgactaccc ggatgcaacg gtaatactcc
     1741 ttgaagagaa ctaccgctct actaaaacaa tcattcaatc agcgaacgaa attattgcaa
     1801 aaaataaata ccgaaaacca aaaaatttat ttactaacaa ccaggatgga gaatctattt
     1861 ctctctactg cgcgtatgat gaagcagacg aagcgcgctt tattgttgag cgcgcaatag
     1921 agcagttaga caaaggagtg cgagctgaag aaattgccgt actctaccgc accaactttc
     1981 aatcacgcgt ccttgaagag gcattcttaa acgcacacat tccataccac gtgttagggg
     2041 tgcgattttt tgagcgaaaa gaaatcaaag atgctgtttc atatttgcgc gcagcgctca
     2101 cccccgagag cacgcttgat attaaacgca cgattaacac gcccgcccgc ggcatcggaa
     2161 agacaacact cctcaaagta ttttctggtg acagagaagc attgcccgca aaagcaaaag
     2221 ctgcggttgc tgcctactat gcactgcttg attctgttcg tgcgttcgct gaagcacatc
     2281 ccgtttcaca tacattaaag tacattatta ccgagagcgg actcgagaaa cactaccagg
     2341 cagagcctga cggcgaagaa catttggaaa acttgcgcga gctggtaacg ctttcgctga
     2401 agtacgacca cctgcccgga atagcaggtg ctgaaaaact aattgaagat gcaacacttg
     2461 cctccgacca agactcgctc gagtcgaaag atgaaaaaaa gaagggggta aaactaatga
     2521 ccatacacgc atcgaaaggg cttgagttta atgttgtgtt tgtagcaggg cttgaacatg
     2581 ggctctttcc gcatgagcgc gatgcaaagc aagctggtcc tgatggcgaa gaagaacgcc
     2641 ggctgatgta cgtagcgctc acccgggcac gacaaaaatt atttttaagc tacgcatcga
     2701 tgcgcactat ttttggcatg cgcgatgtgc ggctgccctc tgaattttta ctcgacattc
     2761 cagaagagct cattacccga gaaacacgag acggggagtt tacggtgtat ctataaaaaa
     2821 ttcccgagta gtctcgggaa ttttttattt gtttagaacg aggtgcctgt tcctggattt
     2881 gaatccattg tgccgtctga ttttacctct tcacattctt cacccacgta ctgctcgcac
     2941 tgtccaccgg acgagtacga acccatcagg tacgtaccgt agatgttgtt gttcgcgtac
     3001 agttttgtag aactgtcata aataagtgca agcgatgtat ttgaaacaac atcttgaata
     3061 taaataagcg tgttgttggt gcaggtgcac gagagtgagc cagtgcggcg gccagcgtag
     3121 aagttgccac cgaatgcttt aatgcgctgt acttggaagg taaggataat agcggcaacg
     3181 aggagtacgc cgaggagtac aagaagaatg tatttgataa ttttttttgg gtgaggctgt
     3241 tgtagtggtg gcatggcaaa agtataccat gggagcacgc aggcaacttc cttgtcattt
     3301 attcaattca ggagtaagaa gtaggtacat gatgcacgca aaaaaacggt tcgggcagca
     3361 ctttttaaaa tcggcagccg cactcgcaca catggttgcc gcaagtgcgc ttactgaaaa
     3421 agataccgtg cttgaaattg gtccgggcac cggcatcctc actgaaaaac tgctcg
//
LOCUS       DATIBQ010000059        13861 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U16294
            k141_18445, whole genome shotgun sequence.
ACCESSION   DATIBQ010000059 DATIBQ010000000
VERSION     DATIBQ010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935789
            Sequence Read Archive: SRR25158331
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 13861)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13861)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:58:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 637
            CDSs (total)                      :: 603
            Genes (coding)                    :: 601
            CDSs (with protein)               :: 601
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13861
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18445"
                     /isolate="SMAG_U16294"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1597
                     /locus_tag="VLB02_01820"
     CDS             <1..1597
                     /locus_tag="VLB02_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HSE56802.1"
                     /translation="TPGHADFGSEVERVLRSIDSVLLIVDAQEGPMPQTRFVLKKSLE
                     LGLKPIVVINKIDKPAADCAKTHDGVFELFLELGATEEQLDFTTVYAIGRDGIAKKNL
                     SDESTNLDPLFETILEKVPVAANDMAKPFRMQAFNLAYDNFLGRMGVGRVYEGVAKTG
                     DMVFIKSHTNAARTAKITKIFSFHGMKREEVTEAVAGDIVMLAGIPDIFIGETIAKTA
                     DAELLPAITIDEPTIVLDFLVNNSPFAGKDGKFVTSRQIRERLERELEVNVGLKVDFT
                     PDPNRIAEGFKVYGRGEMHIAVLLETMRREGFEMQVSQPQVIVKEIDGVKCEPFEEVT
                     IDTPSEFQGPIIEKLGGRGFIMSDMKVHGNSTRMLFEGPTRGLFGYRGQFMVDTKGEG
                     IMASRVIGFKPYAGEITRRHVGSMVSMENGKALAFALWGLQDRGVLYIGPQTEVYEGM
                     VIGNTSKGEEMKVNPTKGKQLTNMRASGTDEAVTIIPPLTMTIERGLEVITDDEFLEV
                     TPNYVRLRKQYLTETDRAKAKRK"
     gene            1674..2375
                     /locus_tag="VLB02_01825"
     CDS             1674..2375
                     /locus_tag="VLB02_01825"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HSE56803.1"
                     /translation="MKKKQLKDFWNEEYRDPGFFALSTVVSADLEKWVRFMQKEFGKD
                     VFRQGLTVLDLGCGNGRNLLYLCEQFNMKGIGYDISEEGIRQAQKASDNIKDRVKFHV
                     RSIGDQFPLENESVDIVLDMMTSHFLKEDERQVYLQELLRVLKPGGFLFFKSFYAPGD
                     LHAKRLVAEHSAGEKNAYIHPKMKVYEYVWTDEALQAFFGPHFELLEKHASHKHFNRG
                     KPNKRRSIVCYFQKK"
     gene            2585..2660
                     /locus_tag="VLB02_01830"
     tRNA            2585..2660
                     /locus_tag="VLB02_01830"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2618..2620,aa:Thr,seq:cgt)
     gene            2883..4685
                     /locus_tag="VLB02_01835"
     CDS             2883..4685
                     /locus_tag="VLB02_01835"
                     /inference="COORDINATES: protein motif:HMM:NF025253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098024 - virus tail, fiber [Evidence
                     IEA];
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - viral entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HSE56804.1"
                     /translation="MYKKNAAIVTLFIFFVALIFPRLVGATIYQPGETLEPDCAPGSV
                     NCGVAVLELNGETDTTQTMAVGSSGTDFSIASVGGVHSFNIPSASALARGLLTAANWS
                     TFNSKESAITAGTTSQYYRGDKTFQTLDTAAVPENGNLYYTTARFDTRFATKTADDLV
                     AGLVNKYYTTSLFNTDFGTKDSDDVAEGSTNLYFTNARARSALSVGGAPLSYDSSTGV
                     FTISQAATGTSGYLSSTDWNTFNNKQAALGYTPLNVASNLSDLANASTARTNLGLGTL
                     ATLSSINNSNWSGAGLSVANGGTGAISLNDLITLATHTTGNYIATISGSSQISVSGSG
                     SENAAATLSIVADSIGDSQLAFNTGQTLTTAASPSFTGLTISGTTGCDGSLEALGTDG
                     SGVVSCHNIALSDARLKTNIAPVDASMGLALINELTPVEYNWIDPNVYGGNTQKQYGF
                     IAQDALTVVPELVGTTTPTELTPDHTYFFNYIGLISPIVKAIQELSNKVSDTAHLVLS
                     RLTVHDTFCINETCITEDDLKLFLAQKNTDPVIDQIPEDDTSTKIPVPVIESTPVVVG
                     VPSAPSETEAVIPAVVELVVPSSESITFPEIASQ"
     gene            4692..6020
                     /locus_tag="VLB02_01840"
     CDS             4692..6020
                     /locus_tag="VLB02_01840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56805.1"
                     /translation="MKNQKPLLIVGYALCLFFSVTQVHAATILSTHTYAWSDQVGYIN
                     FEHVTVEDDALAGYAWSANKGFIVFDPLLGGVANDGSGNLSGAAWGEQLGWIDFGNVN
                     IDTDGVFSGTATGTLVGTLTFDCPTYCDVQTDWRAPASSTLSSSSGRISGSLVAQASS
                     AFSENVPNTPFNSIPSQQSESSEELSVTKKSIELVFPNEPVETPALFDVISELLVSKV
                     APGDDLPVLVKLSNFGGNKRVDVLIAYSVITNHGVELYKTTETVAVETTANFVKKIHI
                     PVTTAPSSYTIQTAITYEGQLVSATAQLPFTVERKILGLFQSSFYRYSAIAAVAAGLA
                     WLFGYLWIRRRRNARLEPIDYSAIPRGERTFYEIISDTITQMRGRVGDEALTIAAQIP
                     GLEIDHDSGRVLAITGPPAKIVATLVSEYETLLGKKVSFSFRKETAIKKE"
     gene            6030..6434
                     /locus_tag="VLB02_01845"
     CDS             6030..6434
                     /locus_tag="VLB02_01845"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSE56806.1"
                     /translation="MNTQKTILIVEDEKDIREALRDVLENNQYLVLEAKNGKEGVTAA
                     LEHHPDLILLDLLMPEMDGMAALTTIRSDVWGKDAPVIILTNLNANNEHLVADVVSHK
                     PLYYLIKSDWNIGDVLKKIEETLNVSSEALTH"
     gene            6439..8667
                     /locus_tag="VLB02_01850"
     CDS             6439..8667
                     /locus_tag="VLB02_01850"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HSE56807.1"
                     /translation="MKLFKAPIISRKEIAEHTVEVTFAVPAHDFEFQAGQYVRITIPE
                     LTIPDPRGNYRDFSIVSSPNERAAFTIAVRASESGFKKTLLELPLGSEVQVTGPHGEF
                     TLPKNPDRPIVYIAGGIGITPFMSHFQFAFEEQLAYKFIVLYSNTALANTPYLAELDN
                     FAKQHHDFTVEKIFAPIEEKHIRERIMGVMEITTPLWFLAGPPAMISLVRKILLALGV
                     SEGTIHIEEFLGYESGSSIPSQAIARRVQNREEVLKLQVARAETLLDALSKIALISED
                     DLEGTITFANDKFVEIAKYKREEIMGQNHRILKSGYHPPEFFKEMWATISSGRVWHGE
                     VKNMAKDGTYYWVDSVIAPVTGDEGRPVSYLSVRFPITERKELEEVKAKDEAVLASIG
                     DGLAATDEEGKIVLVSKSFTKLLGWEPAEVKGRLFTDVVQAVDENGSAIPESERLIYK
                     IFRQHTKLMIADAPSVTTDGMNIYYKRKDGTRFPVGITVSPILIGNRFAGAVEVFRDV
                     TKEKGIDQAKTEFVSLASHQLRTPLTTVNWYSEMLLRTEIGKSIEKQKEYLEKIHSGS
                     QRMVGLVNTLLNVSRIELGTLSVESQPVDVAQFIQEVIKEQDQQIDEKKITITTNIAK
                     DMPVIETDPTLLRMIFQNLLSNAVKYTAAGGRVGIAVSVDKATVLVAFSDTGYGIPKK
                     DQDKVFTKMFRADNVKEKGEGTGLGLYIVKSVLSALGGSIQFTSEEQKGTTFYLTFPL
                     TH"
     gene            8692..9099
                     /locus_tag="VLB02_01855"
     CDS             8692..9099
                     /locus_tag="VLB02_01855"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSE56808.1"
                     /translation="MNTSTSEKKIVIEAIEDDALLRTVLRDKFSGEGFQVLEASDGKE
                     GLATALREKPDLILLDIVMPEMDGITMMQKLRQENEWGKHVPIILLTNLSADNEKINQ
                     AIADNEPAYYLVKSNWTMSDLVEKVRERLSRPC"
     gene            9108..10037
                     /locus_tag="VLB02_01860"
     CDS             9108..10037
                     /locus_tag="VLB02_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_155876544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PCRF domain-containing protein"
                     /protein_id="HSE56809.1"
                     /translation="MDRFLFLYFFGTVSRYMDKEAIAKQIEIIEAEMVGPYFWNDVPA
                     AQAKIKELQELKDKLEGFGKYDRGNAIVTIFSGAGGDDAEDFSAMLLRMYLKYIERQG
                     WSTAMLHEHKNDHGGYRNVTFEISGKSAYGTLKNESGVHRLVRVSPFNANAKRHTSFS
                     MVEVVPKLTKTDTDVVIPETDLEVDFARAGGPGGQNVNKRETAVRLTHKPTGLTVHVA
                     TERSQEQNRDRALELLKGKLYKRLEEERKAAEAGLHISKTTEIEWGSQIRSYVLHPYK
                     MVKDHRTGVEVHNTESVLEDGELEPFIEAEKAL"
     gene            10053..10745
                     /gene="ftsE"
                     /locus_tag="VLB02_01865"
     CDS             10053..10745
                     /gene="ftsE"
                     /locus_tag="VLB02_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HSE56810.1"
                     /translation="MITFVNVSKIYQNGAVALEDVSLEIEKGEFVTIVGPSGAGKTTL
                     SKLVLAEEYPTEGTVLFENVDINALPSRSITGLRRRIGTIFQEFRLLQNKTAYENIAF
                     AMEAAGKSDSEIVSDVPHVLQLVDLQDRMFHFPSQLSGGEKQRLAIARAIINQPEVII
                     ADEPTGNLDAGNTLEVVHILKKINELGTTVILTTHNKEIIDSVGRRVIALEKGRLVSD
                     EVREIKESKRKK"
     gene            10760..11683
                     /locus_tag="VLB02_01870"
     CDS             10760..11683
                     /locus_tag="VLB02_01870"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HSE56811.1"
                     /translation="MFWITIRRIIKSGWINFKRSGLISTAAVLITTITLSVITALILF
                     QAMLTGAIEVVKNKVDITVFFSINTSEDQILTLKQQLEQLPEVAVVDYTSADQEVLEF
                     RERHANDYLTLQALDELGDNPFGGSFRIKAVDSGQYESIAALLEGDSALAKDNATLIE
                     RVNYSQNKQAIDRLNALIAGSREGGLAATILFIIISVTIMFTTVRLTIYTVREEIGIM
                     RLVGASKMYARGPFIVEGFLYGFFAWLITIALFFPLTYLVNRGAGELLGFDVYHYYIA
                     HLAVLGGITFVVAAALGFLSSWLAVRKYLNV"
     gene            11685..13382
                     /locus_tag="VLB02_01875"
     CDS             11685..13382
                     /locus_tag="VLB02_01875"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008083617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HSE56812.1"
                     /translation="MKKPSSPKYIFVVGGVISGVGKGITASSIAKILQDRGLTVTALK
                     IDPYINIDAGTMNPTEHGEVFVLPDGDETDQDMGNYERFLDINLTSANYMTTGRVYQS
                     VIEQERALKFGGACVQVVPHIPLEVIARIQNAQKHARADVVIVEIGGTVGEYENILFL
                     EAARMLKIRHPRDVMFVMVSYLPTPSKIGEMKTKPTQHSVRTLNSSGLQADLIVARSH
                     IALDEKRKQKLSFFCNVPVEHVISAPDIDTIYEVPMNFEKQKVPETIANILDLKLSKT
                     VSPRAKEWATLVKKIKNGHEEVRIAVVGKYFDTGDFTLTDSYISVIEAIKFSAFSQNR
                     KPKLEWISSEQFEKPVRPNGRSGGDPKSVKLLAEYDGVLVPGGFGSRGVEGKIAAIKF
                     ARENKIPYFGLCYGMQLAVVEYARHMAGIKGATTREINPKSTECVIDVMDSQREKLKK
                     EHYGGSMRLGSYEALIKPKTLARELYNDATISERHRHRFEVNPEFIPALEKAGLVFSG
                     TSPDGTLMEIAELPTSKHPFFLGTQFHPEFQARPLSPHPLFTGFIKAAIKYGKSRKR"
     gene            13392..13823
                     /locus_tag="VLB02_01880"
     CDS             13392..13823
                     /locus_tag="VLB02_01880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56813.1"
                     /translation="MQHVAIMNKSWKLIPKILSKEKTIESRWYKTKRTPWGKIKPGEV
                     IFFKNSGELVTAKAMVKKVLQFEIKHLVDAEKIVQTYGKEICIINKNPRTWGKLPKYC
                     ILMFLTNPQIVKIPFAIEKRGFGTGAAWLTVPNVLKLKKLE"
BASE COUNT         3876 a         3033 c         3456 g         3496 t
ORIGIN      
        1 tacgcccggc cacgccgact ttggcagcga ggttgagcgc gtgctccgct cgattgactc
       61 ggtgctcttg attgttgatg cccaagaggg cccaatgcca caaacacggt ttgtgcttaa
      121 aaaatcgctc gagctcggcc ttaagccgat tgtggtgatc aataaaatag ataagcccgc
      181 agccgattgt gctaaaacgc atgatggcgt cttcgagctt tttttggaat tgggtgcaac
      241 tgaagaacag ctcgacttta ctaccgtgta cgctattggc cgcgacggca tcgcaaaaaa
      301 gaatctttct gatgaatcaa caaaccttga tccgctgttt gaaacaattc tagaaaaagt
      361 gcccgtagcg gcaaacgaca tggcaaagcc gttccggatg caggcattta atttggcata
      421 cgataacttt cttggtcgca tgggtgtggg gcgcgtgtac gaaggggtag caaaaaccgg
      481 agatatggta tttattaaat cgcacaccaa tgcagcgcga actgcaaaaa tcaccaagat
      541 tttctcattc cacggcatga agcgcgaaga ggtaaccgaa gcggttgcag gtgatattgt
      601 gatgctcgct ggtatccccg atatttttat tggcgagaca attgcaaaaa ctgctgatgc
      661 agaactcctc cctgcaatta cgattgacga accaacgatc gtgctcgatt ttttggtgaa
      721 caattcaccc tttgccggca aagacggcaa gtttgtaacc agtcgccaaa tccgcgaacg
      781 cttggagcga gaacttgaag taaacgtagg gttgaaggtt gattttactc ctgacccgaa
      841 tcgtattgcc gaaggcttta aggtgtacgg tcgcggcgag atgcacattg cagtgctctt
      901 agaaacaatg cgccgcgaag gatttgaaat gcaggtatcc cagccgcagg taattgtgaa
      961 agaaattgac ggggtgaagt gtgagccgtt tgaagaggta acgattgata ccccgagtga
     1021 gttccaaggt ccgattatcg aaaaactcgg tggtcgggga ttcatcatga gcgacatgaa
     1081 ggtgcacgga aatagtacac ggatgctctt tgagggacca actcgcgggc tctttgggta
     1141 ccgcgggcag tttatggtgg acaccaaagg cgaggggatt atggcgtccc gcgtgattgg
     1201 gttcaagccc tatgccggtg agattacccg ccgccacgtg ggcagcatgg tctcgatgga
     1261 gaatggcaaa gcgctcgcct ttgccctctg ggggctccag gaccgcggtg tgctctacat
     1321 tggtccgcaa accgaagtgt atgaaggtat ggtgattggg aatacttcaa aaggagaaga
     1381 gatgaaggtg aacccaacaa aaggcaagca gcttaccaac atgcgtgcta gtggaaccga
     1441 tgaagcggtt acgattatcc caccgctcac catgaccatt gaacgagggc ttgaagttat
     1501 tacagacgat gaatttttgg aagtaacccc aaactacgtt cggctccgga agcaatattt
     1561 aactgaaacc gatcgagcaa aagcgaagcg aaaataaaac accgcagata gcttctgcgg
     1621 gttttatttt tagatgaagt aatacaatct ctgcgtataa taggagaatt actatgaaga
     1681 aaaagcagct caaagatttt tggaatgagg aataccggga tccggggttt tttgcgcttt
     1741 caacagtggt gagtgctgac cttgagaagt gggtacgctt tatgcagaag gaatttggga
     1801 aggatgtatt ccgtcagggg ctcaccgtgc tcgacttagg ctgtggcaat gggcgcaacc
     1861 tgttgtatct gtgtgaacag tttaatatga agggtattgg ctacgatatt tctgaagagg
     1921 gtattcgcca ggcacagaag gcatctgaca acataaaaga ccgggtaaag ttccacgtgc
     1981 gctcaattgg cgatcagttt ccactcgaaa atgagtcggt ggatatagta ctcgatatga
     2041 tgacctcgca cttcttaaaa gaagacgagc ggcaggtgta cctgcaagag ctgctccggg
     2101 tgctcaagcc gggcggattc ttattcttta aatcgttcta cgcaccaggc gacctgcacg
     2161 cgaagcggtt ggtagccgag cacagtgccg gggagaaaaa tgcgtatatt catccaaaaa
     2221 tgaaggtcta cgaatatgta tggacagacg aagcactaca agcatttttt ggtccacact
     2281 ttgagctgct tgaaaagcat gcttcacata agcattttaa tcgcggcaag ccaaacaaac
     2341 gacgttcaat agtctgttat ttccaaaaga aatagccaac aactgattag tcgtcggcta
     2401 ctatgtacga tgggagtata ggcgtatctt acccggagtg cccaatagct ctcatcagag
     2461 gcaggggtaa taaataaggt aaaggggtgc ttggtttgga ctgtatactc attcctttac
     2521 ttttgcaagt gttttgaaaa tacctcgttt ttcgtgtagt atggggcgtg cacgagtaaa
     2581 tagcgccacc ttagctcagt tggcagagcg gcggtatcgt aaaccgcagg tccgcagttc
     2641 gatcctgcga ggtggctcca taaaaatcag agatttttat ccaagctgga tcgaaccaga
     2701 agggggtcgg ggaaactggg tgtttccccg tagaggaagg cagcgagtct tcgagcgttg
     2761 gaaaaccgtg ggtttctaag gggtgatatc ctgcgaggtg gctcactaaa aattaaatac
     2821 tttttcattt ttcttttaat ctgaagcatc atacttatgg agcagataat gccataagta
     2881 cgatgtataa aaaaaatgca gcaattgtta cgctttttat attttttgtt gctcttattt
     2941 tcccgcgttt ggttggtgca accatatatc aaccaggtga gacgcttgaa ccggattgtg
     3001 ccccgggaag tgttaactgt ggtgttgcag tcttggagtt aaatggtgaa acggatacta
     3061 cccaaacgat ggcagttggc agtagcggta ctgatttttc aatcgcttca gttggtgggg
     3121 tacactcatt taacattccc tctgcctcgg cgcttgctcg ggggcttttg acggctgcta
     3181 attggagtac ttttaatagt aaagaaagtg ctattacggc tggtactact agccagtact
     3241 atcgcgggga caaaacattt caaacgctcg acactgctgc agtgccagaa aacgggaatc
     3301 tctattacac cactgctcgt tttgacacgc gttttgccac taaaacagct gatgatttag
     3361 tagcaggtct tgtaaacaag tactacacaa cctcactttt taataccgat tttggtacaa
     3421 aagattctga tgatgtagct gagggtagta caaatttgta ctttaccaac gctcgagcac
     3481 gaagcgcact ttcggttggt ggtgcgccgc ttagttatga cagcagtacc ggagtgttta
     3541 ctataagcca ggcagcaaca ggtacgagcg gttacctttc atcaactgac tggaacacct
     3601 ttaataacaa acaggcagca cttgggtata ccccgctgaa tgtggcaagt aatctctctg
     3661 atctggcaaa cgcaagcacg gcgcgtacga acctggggct gggaacattg gcaacacttt
     3721 cttctattaa taatagtaat tggtcggggg cagggctttc ggttgctaat ggggggacag
     3781 gggcaatttc gctgaatgat ttaattacgc tcgctactca tactacaggg aattatattg
     3841 caactatcag cggttcatcg cagatctctg tttctggatc gggaagtgaa aatgctgctg
     3901 ccaccctttc tattgttgct gattctattg gtgatagcca gcttgcattt aacacggggc
     3961 agacgctaac cactgctgct tctcccagtt ttactggtct tactataagc ggcactacgg
     4021 ggtgtgatgg ctcgctggaa gcgcttggta ctgacgggag cggagtagtt tcttgtcata
     4081 acatagccct ctcagacgcg cggctaaaaa caaatattgc accggttgat gcttcaatgg
     4141 ggctcgcgct tattaacgaa cttactccgg ttgaatataa ctggatagat ccaaacgtgt
     4201 atggaggtaa tactcaaaaa caatatggct ttattgcaca agatgcgctt acggtagtac
     4261 cagagttagt tggcacaaca acgcctactg aactcacacc cgatcatacc tattttttta
     4321 attacattgg cttgatatct ccgatagtaa aagcaattca agagctttca aataaggtga
     4381 gtgatacggc acatcttgta ctttctcgtc ttactgttca cgacacattt tgtattaacg
     4441 aaacctgcat tactgaagat gatcttaaat tatttcttgc acaaaaaaat actgatcctg
     4501 taatagatca gataccagag gatgatacct cgaccaaaat cccagtacct gtaattgagt
     4561 ctacacctgt tgtagtaggt gtgccttcag cgccctctga aactgaagca gtgattccgg
     4621 ctgtggtcga gttagtcgta ccatcttcag aaagtattac ttttcccgaa atagcttcgc
     4681 aataattttt tatgaaaaac cagaaaccat tactcatagt aggatacgct ctctgtctat
     4741 ttttttctgt aacgcaggta catgccgcca ctattttaag tacacataca tacgcctgga
     4801 gcgaccaggt gggctatata aattttgaac acgtgacggt tgaagacgat gcacttgccg
     4861 gatatgcttg gtcggcgaat aaaggattca tcgtctttga cccactgctc ggcggcgtag
     4921 caaatgatgg aagtggcaat ctctcgggtg ctgcctgggg agagcagctc ggctggattg
     4981 attttggaaa tgtgaatatt gatacagatg gcgtattttc tggcactgct accggcacac
     5041 tggtgggtac tcttacattt gattgtccca cctactgtga tgtccaaacc gattggcgag
     5101 caccagcatc tagtacactc tcgagcagta gcgggcgtat ttcaggatct ctcgtggcac
     5161 aggcaagcag cgcattttca gaaaacgttc ctaatacccc atttaattca atcccatctc
     5221 aacaatcaga atcatcagag gaattgtctg taaccaaaaa aagtattgaa cttgtttttc
     5281 cgaacgaacc agttgaaact ccagcactgt tcgatgttat ttccgagttg ttggtttcaa
     5341 aggtagctcc gggggatgat ttgccagttt tggtaaaatt atcaaatttt ggaggcaata
     5401 aacgggttga tgtgcttatt gcgtattcag ttattacaaa ccacggtgtt gaactgtata
     5461 aaacaactga aacagtagca gttgaaacaa cagcaaactt tgtaaaaaaa atacacatcc
     5521 ctgttactac ggcgccgtcg agttatacca tacaaaccgc tattacctat gaggggcagt
     5581 tggtgtccgc tactgcgcag cttccgttta cggtcgagcg aaaaatactc gggctcttcc
     5641 aaagtagctt ttaccggtac agcgcgattg cggcggttgc tgctggactg gcgtggctct
     5701 tcgggtatct gtggattcgc cggcggcgaa atgcacggct tgagccgatc gattactcgg
     5761 ctattccgcg cggcgagcgt accttctatg aaattataag tgacaccatt acccagatgc
     5821 gtgggcgcgt tggcgacgaa gcactcacta ttgcggcaca gattccgggg ctcgaaattg
     5881 atcacgactc tggcagagtg cttgccatta caggaccacc ggcaaaaatt gttgctacgc
     5941 tcgtatccga atacgaaacc ctgctcggca aaaaagtaag cttctcgttt cggaaagaga
     6001 cagctattaa aaaagagtaa ccaacattta tgaacacaca aaaaacaata ctcattgtag
     6061 aagatgaaaa agacattcgg gaagcgttgc gtgatgtgct tgagaataat cagtacctgg
     6121 tgctcgaagc aaaaaatggt aaagaggggg ttacggcagc gcttgagcat catcctgatc
     6181 tcatcttgct cgatctcttg atgcctgaaa tggacggcat ggctgcactc actactattc
     6241 gttccgatgt ctggggaaaa gatgctcctg ttattattct tactaattta aacgcaaata
     6301 acgagcatct tgttgcagat gtagtgtcgc acaagccact ctactacctt ataaaatcag
     6361 actggaacat tggcgatgtg ttaaagaaaa ttgaagaaac actgaacgtg tcgtcagagg
     6421 cactaacaca ctgattgtat gaaattattc aaggctccta taatcagcag aaaagagatt
     6481 gccgaacata cagtagaggt aacttttgct gttccggcgc acgattttga gtttcaggcg
     6541 gggcagtatg tgcgcattac tatacctgaa ctgaccatcc ctgatccacg gggcaactat
     6601 cgagattttt caattgtctc ttcaccgaat gagcgcgcag cgtttaccat tgcggtgcga
     6661 gcatcagaga gcggtttcaa aaaaacgctt cttgagctgc cgctcggttc cgaggtgcag
     6721 gtaacaggtc cgcacggaga atttactttg ccaaaaaatc cagatcgtcc tattgtctac
     6781 attgccggcg gtattggtat tacgccgttc atgagccact ttcagtttgc atttgaagaa
     6841 cagcttgctt acaaatttat tgtgctctat agcaacactg ctctcgccaa tactccttac
     6901 cttgccgagc tcgacaactt tgcaaaacaa catcacgatt ttacggtaga aaaaatattt
     6961 gccccgattg aggagaaaca cattcgagaa cgtattatgg gggtgatgga gatcaccact
     7021 ccgctctggt ttcttgccgg accacctgcc atgatctcgc tcgtgcgaaa aatcctcctt
     7081 gcgcttggtg tgtctgaagg tacgattcat atagaagaat tcttggggta cgaatcaggg
     7141 tcgagtattc cctcgcaggc tatcgcgcgg cgggtgcaga acagagaaga ggtgctgaag
     7201 ctccaggtag cgcgcgctga aacgctcctt gatgcgctca gtaaaattgc actcatcagt
     7261 gaagacgatc ttgagggaac gatcaccttt gcaaacgata aattcgtaga aattgccaaa
     7321 tataaacgtg aagagattat ggggcaaaac caccgtattt taaaatcagg gtaccacccg
     7381 cctgaattct tcaaagaaat gtgggcgact atttcaagcg ggcgtgtgtg gcacggtgag
     7441 gtaaaaaata tggcgaaaga cggaacttac tactgggtcg attcggtgat tgctccggtt
     7501 accggagacg aggggagacc ggtgagctat ctttcggtgc ggtttccgat taccgagcgt
     7561 aaagagcttg aagaagtaaa agcgaaagat gaagcggtcc ttgcatcaat cggcgacgga
     7621 ttagcggcaa cagacgagga ggggaaaatt gtactggtca gtaaatcatt tactaaactg
     7681 ttagggtggg aacctgccga ggtaaaaggg aggctattta ccgatgtggt acaggcagtt
     7741 gatgagaacg gcagcgctat tcctgaatca gagcggctaa tatataaaat ttttaggcag
     7801 cacacaaagc tgatgattgc tgatgctccg tcggtaacca ccgacggcat gaacatctac
     7861 tacaagcgca aggacggaac ccggtttcct gtcgggatta ccgtctcgcc gattttaatt
     7921 ggcaatcgtt ttgcaggtgc agtagaagtg tttcgtgatg ttacaaaaga aaaggggatt
     7981 gatcaggcaa aaactgaatt cgtctcgctc gcctcgcacc agctgcgcac gccgctcacc
     8041 accgtcaact ggtacagcga aatgctcctc cgaaccgaga tcggaaagag cattgagaaa
     8101 cagaaagagt accttgaaaa aattcattcc ggcagccagc gcatggtggg gctggtgaat
     8161 acactcttaa atgtttcgcg gattgagctc ggaacacttt cggttgaatc tcaaccggtt
     8221 gacgttgcgc aatttatcca agaggtgatt aaagaacagg accagcagat tgatgaaaaa
     8281 aagataacga ttaccacgaa tattgccaaa gatatgccgg tgattgaaac cgacccaacc
     8341 ctcctgcgga tgatatttca aaatctgctc tcaaatgccg taaagtatac cgctgcaggg
     8401 ggtcgggttg gtatagcagt atcagtagac aaggcaactg tgctagttgc attttctgat
     8461 accggatacg gcattccaaa gaaagatcag gataaagtgt ttaccaaaat gttccgtgca
     8521 gacaacgtta aagaaaaagg agaaggcacc gggctggggt tatacattgt aaagtcggtg
     8581 ctcagtgcgc tcgggggcag tattcagttt acctctgaag aacagaaagg gactaccttt
     8641 tacctaacgt ttccacttac ccattaatta gttaaatgta tagtaaatat gatgaataca
     8701 tctaccagtg aaaagaaaat agttattgaa gcaattgaag atgacgcact cttgcgcacc
     8761 gtgctccgcg ataaattttc aggcgagggg ttccaggtgc ttgaggcgag tgacggcaaa
     8821 gaggggttgg cgactgccct gcgtgaaaaa cccgatctca ttctgcttga tattgtgatg
     8881 cctgagatgg acggtattac gatgatgcaa aaattgcggc aggaaaacga gtggggtaaa
     8941 catgtgccaa ttatcctgct caccaacctg agtgccgata acgaaaaaat aaaccaagcg
     9001 atcgctgaca acgagcccgc ctactacctc gtaaaatcaa actggacgat gtctgacctc
     9061 gttgaaaaag tccgcgaacg tctctctcga ccgtgctaat ctgaaaaatc gatcgttttt
     9121 tatttctcta cttttttggt acagtgagtc ggtatatgga taaagaagca attgcaaaac
     9181 agattgaaat aattgaagct gaaatggtgg gtccatactt ttggaacgat gtaccggcag
     9241 cacaagcgaa gattaaagaa ctgcaggaac tcaaagataa acttgaagga tttggcaagt
     9301 atgatagagg taatgcaatt gtaactattt tttcaggtgc gggcggggat gatgccgagg
     9361 atttttcagc aatgcttttg cgaatgtatc taaagtatat tgagcgccag ggctggagca
     9421 cggcaatgct tcacgaacac aagaatgacc atggtgggta tcggaatgtt acatttgaaa
     9481 tttcagggaa gagtgcgtat ggaactttaa agaacgaatc gggagtgcat cggctggtgc
     9541 gggtgtcgcc gtttaatgca aatgcgaagc ggcacacctc gttttcaatg gtcgaggtag
     9601 tgccaaaact tactaaaacc gataccgatg tagtgattcc tgaaactgat cttgaggttg
     9661 attttgcccg cgccggaggt ccgggcgggc aaaatgtgaa caaacgcgaa acagcagtac
     9721 ggctcaccca caaaccaaca gggctcaccg tgcatgtggc aaccgagcgt agtcaagaac
     9781 aaaaccgcga ccgtgcactc gaactgttga aaggaaagct ctataaacgg cttgaagaag
     9841 agcgaaaggc agcagaagca gggctccaca tttcaaaaac aaccgagatt gagtggggga
     9901 gccaaattcg aagctacgta ctgcacccgt acaagatggt gaaagaccac cgcaccggtg
     9961 ttgaggtgca caataccgag agtgtgcttg aagatggcga actagaacct ttcatcgaag
    10021 cagagaaggc gctataatac taccttgtta gtatgattac ttttgtaaac gtctcaaaaa
    10081 tatatcagaa cggcgcagtg gcgcttgaag acgtgagcct tgaaatagag aagggagaat
    10141 ttgtaaccat tgttggtccg tctggtgccg ggaaaacaac tctctcaaaa cttgttttag
    10201 ctgaagagta tccgaccgaa ggcacggtgc tgtttgaaaa tgttgatatt aacgcgctcc
    10261 caagccgttc aattactggc ttgcgccgcc gtatcggtac tattttccaa gaatttcgtt
    10321 tgctgcagaa caaaactgca tacgaaaata tcgcctttgc aatggaagca gccggcaaga
    10381 gtgacagcga aatcgtttca gatgtcccgc acgttctcca gctggttgat ttgcaagacc
    10441 gtatgttcca cttcccgagc cagctctcgg gcggcgagaa gcagcgcctg gcaattgcac
    10501 gcgcgattat caaccagccc gaggtgatta ttgccgatga accaacgggc aacctagatg
    10561 ctggaaacac gcttgaagta gtgcacattt taaaaaagat caacgagctt ggcaccacgg
    10621 tgattttaac tacgcacaat aaagagatta ttgatagtgt tggtcggcgg gtgattgccc
    10681 tcgagaaagg gcggcttgtt tccgacgagg tgcgggaaat aaaagagtca aaacgaaaaa
    10741 agtaggtata atacgaacta tgttctggat cacgatacgc agaattatta aatcgggatg
    10801 gatcaacttc aagcgaagcg ggcttatttc aaccgctgcg gtgctgatta ctactatcac
    10861 tctttcggtt atcacagcgc tcatcctctt tcaagctatg ctcactggcg caattgaggt
    10921 agtaaaaaat aaagttgata ttactgtctt ctttagtatc aacacctctg aagaccagat
    10981 tcttacctta aagcagcagc tcgaacagct gcctgaagta gcggtggtgg attacacttc
    11041 ggcagaccaa gaagtgctcg agtttcgcga acgccacgca aatgactatt taacactaca
    11101 agcactcgat gaattgggcg ataacccgtt tggcggctcg tttcgcatta aagcagtcga
    11161 ctcagggcag tacgaatcaa ttgcagctct cttagaaggc gacagtgcgc tcgctaaaga
    11221 taatgcaaca cttatcgagc gagtgaacta ctcgcagaac aagcaggcga ttgaccgctt
    11281 aaatgcgctc attgccggca gccgcgaggg cgggctggca gcaaccattc tctttattat
    11341 tatctcggta acgattatgt ttaccacggt acgccttact atctacaccg ttcgtgaaga
    11401 aatcggcatc atgcgcctgg taggagcaag caagatgtac gctcgcggac catttattgt
    11461 tgaaggattt ttgtacggtt tctttgcatg gctgattact attgcgcttt tctttccgct
    11521 cacgtacctg gtaaaccgtg gtgcgggcga actcttgggg tttgatgtgt atcactacta
    11581 cattgcgcac cttgcagtgc tggggggcat tacctttgtt gttgccgcag cgcttggttt
    11641 tctctccagc tggctggcag tccgcaaata tctgaacgtc tgatatgaaa aaaccctctt
    11701 ctcctaaata tatctttgtg gttggcggcg tcatctcagg ggtggggaaa ggaataaccg
    11761 cctcttcgat tgcaaaaatt ctgcaagatc gtggactcac cgtaaccgcg cttaaaatcg
    11821 acccgtacat caatattgat gccggcacca tgaacccgac cgaacatggt gaagtgttcg
    11881 tgcttcccga tggcgacgag accgaccaag atatgggcaa ctacgagcgg ttcttagata
    11941 ttaatcttac cagcgccaac tacatgacca ccggtcgagt gtaccagagc gtgatcgagc
    12001 aggagcgcgc tcttaaattt ggcggtgcct gtgtgcaggt ggtgccgcac atcccgctcg
    12061 aggtcatcgc acggattcaa aacgcgcaga agcatgctcg ggctgatgtg gtgattgtcg
    12121 agattggggg cacggtgggc gagtatgaaa atattttatt tttagaagca gcccgcatgc
    12181 tcaaaattcg gcacccgcgt gatgttatgt tcgtgatggt ttcgtacctg cccacgccct
    12241 caaaaattgg cgaaatgaaa accaagccga cccagcactc ggtgcgtacc ttaaactctt
    12301 caggtcttca ggccgattta attgttgccc gcagtcacat cgctcttgat gaaaagcgca
    12361 agcaaaagct ctccttcttc tgcaacgttc cggtggagca tgtgatctcg gctcccgaca
    12421 tcgacaccat ttatgaagtg ccgatgaatt ttgaaaagca gaaggtgcca gaaacaatcg
    12481 ccaacattct tgatttaaaa ctttcaaaaa cggtttcgcc acgggcaaaa gagtgggcga
    12541 cactcgtaaa aaaaataaag aatgggcacg aggaagtgcg gattgcggtc gtcgggaagt
    12601 attttgatac cggtgacttc acccttaccg attcatatat ttcagtaatt gaagcaatca
    12661 aattctcagc cttcagtcag aatcgaaaac caaaacttga gtggatttca tctgagcaat
    12721 ttgagaaacc cgtccgaccg aacggtcgtt cgggcgggga tcctaaatcg gtaaaattgc
    12781 ttgcagagta tgatggggtg ttggtaccag gagggtttgg ttcgcgcgga gtggagggaa
    12841 aaattgcggc tattaaattt gcccgcgaaa acaagattcc gtacttcgga ctctgctacg
    12901 ggatgcagct ggcagttgtt gaatacgcgc gacatatggc aggcataaag ggtgctacca
    12961 cgcgcgagat taacccgaag agtaccgagt gtgtgattga cgtgatggac agccagcgcg
    13021 aaaagcttaa aaaagagcac tacggcggca gtatgcggct cggttcatac gaagcactca
    13081 taaaaccaaa aaccctcgcc cgcgagctct acaacgatgc cactatttct gagcgtcacc
    13141 gccaccggtt tgaggtaaac ccagaattta ttccagcact tgaaaaagct ggccttgtat
    13201 tctcgggcac gagtcctgat ggcaccctta tggaaattgc tgagctgccc actagtaagc
    13261 atcccttctt cctcggcacc cagtttcacc ccgaattcca agctcgaccg ctctctccgc
    13321 atccgttgtt caccggtttc atcaaggcag caataaaata tggtaagtcg cgtaagagat
    13381 aatttttcct tatgcagcat gttgcgataa tgaataagtc atggaaactt attccaaaaa
    13441 tcctttctaa agaaaaaaca attgaatcac gttggtataa aacaaaaaga acgccttggg
    13501 ggaaaatcaa gccaggcgaa gtaattttct ttaaaaattc aggagaactg gttactgcca
    13561 aagcaatggt gaaaaaagta ctgcagtttg aaataaagca cctggtggat gcagagaaaa
    13621 tagtgcaaac ctatggaaag gaaatttgca tcataaataa aaatcctcgt acttggggta
    13681 agctgccaaa gtactgcata cttatgtttc taacaaatcc acaaattgta aaaatacctt
    13741 ttgctataga aaagcggggt ttcggcactg gcgcagcatg gctcactgtt cccaatgttt
    13801 taaagttaaa aaaacttgaa taaatctcgg ttttctagta ttttacgttg gttactatgc
    13861 c
//
LOCUS       DATIBQ010000060         4186 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U16294
            k141_362557, whole genome shotgun sequence.
ACCESSION   DATIBQ010000060 DATIBQ010000000
VERSION     DATIBQ010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935789
            Sequence Read Archive: SRR25158331
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 4186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:58:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 637
            CDSs (total)                      :: 603
            Genes (coding)                    :: 601
            CDSs (with protein)               :: 601
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4186
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_362557"
                     /isolate="SMAG_U16294"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1240
                     /locus_tag="VLB02_01885"
     CDS             <1..1240
                     /locus_tag="VLB02_01885"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HSE56814.1"
                     /translation="AHTSSMKIPMKVLRRFGIPLVTVLSAAGVFLAGLYIGYENRSPI
                     LRAAGVTNKESGVSDEADFAPFWKAWQIISEKFPGAEKVTDQERVYGAIDGLVGSLKD
                     PYSVFFDPQENKEFNEEIAGEFFGIGVEIGKKDNILTVIAPIKGTPAYRAGLLAGDKI
                     IKVGKTFTADLTIDEAIALIRGESGTTVDLTIVREGFKEPKVYTVAREKIIMPTVDTE
                     RREKEKVFIIHLYNFSAQSPTLFAKALQEFARSGDTRLVLDLRNNPGGYLEASVSMAN
                     WFLPEGKVVVKEIGKDPSDVTYHRSKGPGVFAGKIKMAILVNGGSASASEILAGALTE
                     QGVATLIGEKSFGKGSVQELIKLTKDTSLKLTIAKWYTPNGVSISDQGLMPSMVVSQV
                     KDDANVDEQLEAAVKFVAGK"
     gene            1237..1680
                     /gene="rplI"
                     /locus_tag="VLB02_01890"
     CDS             1237..1680
                     /gene="rplI"
                     /locus_tag="VLB02_01890"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HSE56815.1"
                     /translation="MKVILLKGVSKLGKKDDIVEVSDGYAHNALFPQKAAVSATPEAV
                     ARLKKRQAGEQSGKEIQHELLGKALEMLKEKQVTMHVRANAQGHLFAKITATDITALL
                     LREHRLSLEPKVLQLAEPIKETGTYSIPVSFDSFNGSLTLTVAAL"
     gene            complement(1677..1958)
                     /gene="rpmA"
                     /locus_tag="VLB02_01895"
     CDS             complement(1677..1958)
                     /gene="rpmA"
                     /locus_tag="VLB02_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HSE56816.1"
                     /translation="MAHKKAGGAAKNLTDSNAQYLGVKLYDGQAALPGAIIVRQRGTK
                     ILAGKNVGLGKDHTLYALKEGIVKFFSKRKQNFDGTSTVRKAAMVEERA"
     gene            2073..2438
                     /locus_tag="VLB02_01900"
     CDS             2073..2438
                     /locus_tag="VLB02_01900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56817.1"
                     /translation="MNSALRYRLGIVLIIVFVAIGSFLLGTYTKGERGGDGIVLSCDP
                     NTLQALVIPKSSQQSTFVAAASAVVSGSKTYFASKNGKKFYAAGCVSAQKRIKPENVI
                     WFSTAEDATLQGFTEGSSC"
     gene            2448..3353
                     /locus_tag="VLB02_01905"
     CDS             2448..3353
                     /locus_tag="VLB02_01905"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HSE56818.1"
                     /translation="MIRRGIWNAIIVLFVAMSLFFLWPTIKAFSPKAGTLLKQFPGKV
                     SEILKKSFGIGTVRWDDTEEREVKGERDTLPTYEDSSITGIGVATETNRERAAAGLPV
                     LRYNTQLAASAKLKVDDMIAFQYFEHKSPAGVSVSNLGDTVGYTYITIGENLAMGDFT
                     SDADVVAAWMKSPGHKANILSTKYKEIGVAARKAEYHGKVVWFVVQHFGTNRASCPSV
                     DTAAKSAIDALRKQTDALEEKIVTAKHELEQPDAPLEPGYNEAVNAYNNMVDDYNALV
                     QKTKTKVTSYNTQVRTFNQCLARYQ"
     gene            complement(3887..>4186)
                     /locus_tag="VLB02_01910"
     CDS             complement(3887..>4186)
                     /locus_tag="VLB02_01910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56819.1"
                     /translation="VEAHSLFATTATWLYGLLLTGELLAFLTPTLIQKLQVPQVTTFL
                     VFIQKILTHPALSKIITFLGLVAITITGLLGGVMVYGLTADPLAPLVLRILGIQY"
BASE COUNT         1209 a          847 c          986 g         1144 t
ORIGIN      
        1 agcccacacg tcatccatga aaatccccat gaaagtactt cgtagatttg gcataccact
       61 ggtaaccgtg ctttctgcag caggggtgtt tcttgctggg ctttatattg gctacgagaa
      121 tcgctcgcca attttgcggg cagcaggagt aacgaacaaa gagtcaggcg tgagcgacga
      181 agctgatttt gcgccatttt ggaaggcatg gcagattatt agcgaaaaat ttcctggtgc
      241 agaaaaagta accgatcaag agcgtgtgta tggtgctatt gatggattgg ttgggtcact
      301 aaaagaccca tactcggtat tttttgatcc acaggagaat aaagaattta atgaagaaat
      361 tgccggagaa ttttttggca ttggggttga aatagggaag aaggataata tacttaccgt
      421 tattgcgcct atcaaaggaa caccggcgta ccgggcgggg cttttagcag gagataaaat
      481 tataaaagta ggtaaaacat ttaccgccga tctcactatt gatgaagcta tcgcacttat
      541 tcggggcgag tcgggtacaa cggttgatct cactattgtt cgtgagggat ttaaagagcc
      601 aaaagtctac accgttgctc gtgaaaaaat aattatgcca acggttgata ccgaacggcg
      661 tgaaaaggag aaggtcttta ttatccatct ctacaacttc tcggcacaat caccaacact
      721 gtttgccaaa gcactccaag aatttgctcg ttcaggggat acgcggctgg tgctcgactt
      781 gcgcaataac cccggcggat accttgaagc ttctgtgtca atggcaaact ggtttttacc
      841 ggaagggaag gtggtagtaa aagaaatagg aaaagatccg agtgatgtaa cctaccaccg
      901 cagtaaaggt ccgggtgtat ttgccggaaa gataaaaatg gcaattctgg tgaatggcgg
      961 atcagcctca gcgtctgaga ttttagccgg agcacttacc gagcagggtg tagcaacact
     1021 tattggtgaa aaaagttttg gaaaaggctc agtgcaggag ctcatcaaac tcaccaaaga
     1081 tacctcgctc aagctgacta ttgcaaagtg gtatacccca aacggtgtct caatttctga
     1141 tcaggggctc atgccaagca tggtagtgtc acaggtaaaa gatgatgcaa atgttgacga
     1201 gcagcttgag gcagccgtaa aatttgttgc tggaaaatga aagtcatttt attaaaagga
     1261 gtttcaaaat taggaaagaa agatgatatc gtcgaggtga gcgatggcta cgcccacaat
     1321 gcactctttc cgcagaaggc ggcggtgtct gctacgcccg aagcggtggc acgtctcaaa
     1381 aagcggcagg caggcgaaca atctggaaaa gaaatacagc acgagttatt gggcaaggcg
     1441 ctcgagatgc tgaaagaaaa acaagtaacc atgcacgttc gggcaaatgc tcaggggcac
     1501 ctttttgcaa aaattactgc cacagatatt acagcactct tgctgcgtga gcaccgtctc
     1561 tcactcgagc caaaagtgtt acagcttgca gaaccaataa aagaaaccgg cacctattcg
     1621 ataccggtct cttttgattc atttaacgga agccttacgc tcaccgtagc ggcgctttaa
     1681 gctcgctctt ctaccattgc tgctttgcga acagtactgg tgccgtcaaa attttgcttg
     1741 cgcttgctaa aaaactttac aattccttcc ttcagtgcgt agagtgtgtg gtctttaccg
     1801 agccctacgt tctttccggc aagaattttg gttccgcgct gacgcacgat aatagcgccc
     1861 ggaagagctg cctgtccgtc gtagagcttc acccctaaat attgtgcatt tgagtcagtg
     1921 aggtttttcg ccgcaccgcc cgctttctta tgtgccatac aaatttaaaa ataagttaga
     1981 aacctgatta tatttataaa acatctattt gtcaatcatt cctcgaatag ccgattatcg
     2041 cccgtgcttg tcaaccttgg tatacttttc tcatgaattc cgccttacgc tatcgcctcg
     2101 gtattgtgct tattattgta tttgttgcta ttggctcgtt tttgctcggt acatacacca
     2161 agggcgagag agggggtgat ggtattgtac tctcatgtga tccaaacaca cttcaagcgc
     2221 ttgtcattcc taaaagttcg cagcagtcaa catttgtagc ggctgcatca gcagtcgtgt
     2281 ctggcagtaa aacatacttt gcttcaaaga acggtaaaaa attttatgca gcagggtgtg
     2341 tcagcgcgca aaaacggatt aaacccgaaa atgtaatttg gttttcaact gctgaagatg
     2401 caacactcca aggctttaca gagggcagtt cgtgttaata tgtatgtatg atccggcgag
     2461 gtatttggaa tgctatcatc gtgctctttg ttgcaatgtc gctgtttttt ctctggccaa
     2521 caattaaagc attttcacca aaagcaggta cattactcaa acaatttcct ggaaaagtat
     2581 ctgaaatttt aaagaagagt tttggtatcg gtacggttcg ctgggatgac accgaagaga
     2641 gggaagtaaa gggcgagcgt gatacgcttc ctacctatga agatagctct ataaccggta
     2701 tcggtgtcgc tactgaaaca aaccgagagc gggctgccgc agggctgcca gtactccgtt
     2761 ataataccca acttgccgcc tcagcaaagc tcaaggtaga cgacatgatt gcgttccagt
     2821 attttgagca caagtcgcct gccggagtga gtgtttctaa tttaggagac acggttggtt
     2881 atacctacat caccattggc gaaaatttag cgatgggtga ttttaccagc gatgctgatg
     2941 ttgttgctgc ctggatgaag agcccggggc acaaggcaaa tattttaagt accaagtata
     3001 aagaaattgg tgttgccgcg cgcaaagcag agtaccacgg caaggtagtg tggtttgtgg
     3061 tgcagcattt tggcactaat cgcgctagct gtccgagcgt tgatactgca gcaaaaagtg
     3121 caattgatgc actgcgcaaa caaaccgatg cacttgaaga aaaaattgtt actgcaaagc
     3181 atgagcttga acagcccgat gcaccgcttg aacccggtta caacgaagca gtaaatgcgt
     3241 ataacaatat ggttgatgac tacaacgcgc ttgtgcaaaa aacaaaaact aaagttactt
     3301 cctataacac ccaagtccgc acctttaatc agtgtcttgc tcggtatcaa taactaaaaa
     3361 agagggaagc gcgccgtcct aaaaaggtgg ttttattttt tatatccctg tgctcaccta
     3421 ccaaaaaatc ggggaagtaa gaattatttt tttaaaataa aacaataatt aattatgatg
     3481 tatgtaggga gaatttcctt tattgatttc attactatga ccaaagagag gagagagagg
     3541 taaactaata gcctggttat gcttttatat gatgtaaggt aaattaccta agtgtcgcac
     3601 aatatatctt ttacgacact acgttgctct ttttagtctt tatttatata gctatggatg
     3661 catgttatct aattcctttg aaagaaagga actatgtatt cctcgcacaa tgtaattact
     3721 tttcttttac attgtttact atcccttatt ttaaataaaa taactaaagt ttattttatt
     3781 attgactata ttatcaacta attgtacact ggaagtcagt ttaggttagg gtaccaaagg
     3841 gtactttagt agccccagtg tttctacatt ggggcttttt tattttttag tactgtattc
     3901 ctaatattcg cagcacgagt ggtgcaagcg ggtcggcggt gagtccgtac accattaccc
     3961 cgcccaggag tcccgttata gtaatggcaa caagcccgag gaaagtaata atttttgaga
     4021 gagcgggatg ggtaaggatt ttttgaataa atacaagaaa ggtggttact tgcggtactt
     4081 gtagtttttg aataagtgta ggggtaagga atgcgagaag ttcaccagtc aagagcaacc
     4141 cgtagagcca ggtggcagtg gtagcaaaca atgagtgcgc ctcaac
//
LOCUS       DATIBQ010000061        13936 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U16294
            k141_1976604, whole genome shotgun sequence.
ACCESSION   DATIBQ010000061 DATIBQ010000000
VERSION     DATIBQ010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935789
            Sequence Read Archive: SRR25158331
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 13936)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13936)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:58:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 637
            CDSs (total)                      :: 603
            Genes (coding)                    :: 601
            CDSs (with protein)               :: 601
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13936
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1976604"
                     /isolate="SMAG_U16294"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..421)
                     /locus_tag="VLB02_01915"
     CDS             complement(<1..421)
                     /locus_tag="VLB02_01915"
                     /inference="COORDINATES: protein motif:HMM:NF045213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HSE56820.1"
                     /translation="MAGHSKWKQIKHKKAASDAKKSKVFSKLALLIGNEAKAAKGDRN
                     APNLRAAIEKARAENMPNENIERAISKANEQKELDHVTYEGYGPGGVGIVVQALTANR
                     NKASAEIKHAFAKHDGSLGAIGSVTWGFTKEAGTWTPT"
     gene            complement(423..1412)
                     /gene="ruvB"
                     /locus_tag="VLB02_01920"
     CDS             complement(423..1412)
                     /gene="ruvB"
                     /locus_tag="VLB02_01920"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008579113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HSE56821.1"
                     /translation="MSSITPNTLSSSGDEQFSDATLRPNTFDEYVGQENIKKNLSILI
                     RAAKERGHMPEHILFYGPPGLGKTTLAHVIARETGANLKTTSGPTIERVGDLAAILTN
                     LSPGDILFIDEIHRLNKTIEEVLYPVMETGKLDIMIGKGPAARTIALELPAFTLVGAT
                     TRFGMLSSPLRSRFSGGAFRLEFYTPSEISTILRRSGDILGISIKDELLSHIAGRSRS
                     TPRIANYLLRRVRDYAQIHKRELDSSAVEEAFALLGLDALGLSQNDRTILKAIDEKFS
                     GGPVGIKNLAALTGEDEGTIEDVIEPYLIQVGLIERTPRGRVITEKGKEYLKK"
     gene            1646..1933
                     /locus_tag="VLB02_01925"
     CDS             1646..1933
                     /locus_tag="VLB02_01925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56822.1"
                     /translation="MIKVFYKLLIGNTSFTKVCKVHSVPAEGTLLKAANFSFKVFEIE
                     QDLDVYISDGVELGQFSDSVKANFNDEWEMPSGSRLKKLFEDLTKAGWEQK"
     gene            complement(1969..3669)
                     /locus_tag="VLB02_01930"
     CDS             complement(1969..3669)
                     /locus_tag="VLB02_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HSE56823.1"
                     /translation="MPLSFDEEKQNKRLDELKRDEEENLLQMLAETQFHVPYIDLTST
                     SIDNDALRILSETESRQFEVGPFNLVGKKLSLAVHSPTSDGALQARTIAESRGYAVSF
                     FMASRKSIEKVWSRYKDLSYATTTRAGGLDISQDVLVEMAERVKTTDDAIRFITQAIQ
                     SKETHAISRILETILGAGIALGASDVHIEPEADFIRLRYRLDGVLYNIMNFDAHLYQL
                     LNSRVKLLSGLKLTVKEVPQDGRFSSFMNEAEINYRTSLIPGAYGESIVLRILNPKSI
                     QVNLDELGISPELYEIFMREIKKPNGMILLTGPTGSGKTTTLYAFLQKIYTPEIKVIT
                     IEDPIEYHLPGITQTQTDHKKGYTFLEGLRSALRQDPDVIMVGEIRDPETAKIAVESA
                     LTGHLVFSTLHTNNASGVIPRLIDLDVNPKILVSALGCSIAQRLCRKLCTECKYEREV
                     TAEEKVRINTIWEKAAAEGKDLSARSVSREVTKLWSAKGCPHCNNTGYKGRVGIFEAI
                     LTDEAIEKIIMASPTERDIKKASLAQGILDMKEDGLVKILKGITDFPEVMSVVDITED
                     "
     gene            4534..5505
                     /locus_tag="VLB02_01935"
     CDS             4534..5505
                     /locus_tag="VLB02_01935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56824.1"
                     /translation="MTAREQELQVVKQQEDSVRKKNAEFSRMLAAQNDSILFLKERSV
                     GYADMQKKLVVAETKEDSLVREVTSVNVQLVKAAGEQKKLQQQNSFLGSILDSTKKAD
                     STKFVALQQQLAERNKETGNTTTASVNGLGKGILVVIGISIVITGILIFSWAKFGQTR
                     KKQLLIGLAGSAIIIVLTLTFSWFFFSKEPEVVHAEEQEKQELKDSLAKEYQVKLVDT
                     LKKVAKLPVPVVHASDTALATSLERERVLKESLAAKADSLFAVNKEKNAIIDSLKLVT
                     KRDNIADTTQQRSRSSVVIKGPGRYSIPRNQVVVRSKKRNPGKYSLP"
     gene            5673..6647
                     /locus_tag="VLB02_01940"
     CDS             5673..6647
                     /locus_tag="VLB02_01940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56825.1"
                     /translation="MKKLFSSLIIFTVFTVTAQDYLNGTPDGAGFLRKSEYGKHMDEE
                     EVQSFSTSGNTLSISNLKGSKAKEYISAHTLQFLGESSTQAAFGNSKVIGVGELPAGT
                     KISTMDMHGNHILRDPYPGERFYFHTSTGKVWFSLYCWNPVFVTITQPAPTNNNYVGG
                     GNKEFKEEKTSCEISWKTGYAIYSQGRNDREGDMLVDASLYTRIQAVNQCCGGGGNNG
                     GGSGASVYNQASETVRYVQVQNGGGGNTFGSTLLGSAIGTFAGHVVGELLMDGRGQPV
                     IVNQRQGVVLTSDNGGVGGGGNYPSTFTPWHAGTGDGSRGNLPRGYFR"
     gene            6811..8757
                     /locus_tag="VLB02_01945"
     CDS             6811..8757
                     /locus_tag="VLB02_01945"
                     /inference="COORDINATES: protein motif:HMM:NF013508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56826.1"
                     /translation="MKKIIISMFALFGMLIATPILAHAADRFNTDPNDLPTIMVTNVT
                     QDGGCSAGTGAGCWKTSVDARPGDTVAVQVYFHNATNTIAYDTHLSLQPGETSRGTTH
                     TFSGGVASSSIDRATGSATVRTSSTQKLTFINGSVRVFRHGDQVGFSVDNENSLFGTG
                     LSIDEVGVGVQGVLMAKFKVSNDAVTDDDNCRITDFTADDYTIDEDESTRLRWDTTDC
                     DYVKISHISGNLDTDGSVSVSPNEDTKYILTAYPGGDTEDLTIRVNEDSNNNNCDISF
                     YADRTSITRGQSTKLHWDVSDGNDIDISNLDSNVNESGSKTVSPNSTTTYRLTVNGGD
                     CDNESKSVTIRVEDGASTAKPQAITNPATLVAATAAKLNGIAIPNANVNTSAWFEWGP
                     TPALGAATAAQGVGSAGSLAYSYTVGSLMPNTTYYFRAVVQNANGVGLGETLSFRTGA
                     TVTNTTRVVRSTRVVSSAVTARSTASLLELSVENAYDRMCAGGEIEYIVTYKNISRST
                     LVDAVLQVTHPKQLVFVDATRGDYSASERKLTVDLGQLAAGEEGSIRVRGRVTDNSVV
                     GDLIVTTATVVYTNPTTRAQEDAIAYSLTTVSDDCPNGFGAASLFGSGSFLPDTLIEW
                     LLLILVILALMLLGRNLYNKKASA"
     gene            8819..9256
                     /gene="tsaE"
                     /locus_tag="VLB02_01950"
     CDS             8819..9256
                     /gene="tsaE"
                     /locus_tag="VLB02_01950"
                     /inference="COORDINATES: protein motif:HMM:NF014426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HSE56827.1"
                     /translation="MQERFTLFDIPRIARQLLTVLRPVEDRATILALSGELGAGKTAL
                     AQAIGAALGVAEQMVSPTFVLMKQYQTPAGPFTTIIHIDAYRIENEAELLPIHFEALT
                     QQGNTLIIIEWPEHIAAALAGRVVFRYTLSHLESDERMIEQLP"
     gene            9253..11625
                     /locus_tag="VLB02_01955"
     CDS             9253..11625
                     /locus_tag="VLB02_01955"
                     /inference="COORDINATES: protein motif:HMM:NF012688.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HSE56828.1"
                     /translation="MTKKKNKTLVLLDAHAIIHRAYHALPAFTNTAGEPTGALYGLTT
                     MLLRIITDLKPDYLVAAYDLPKPTFRHQAYEAYKGTRVKADDELKAQLTQSREVFDAF
                     GIPHIDAEGFEADDVIGTLVEKFKKEKDLTIIIASGDMDTLQLVEGNRVQVFTLKKGI
                     TDTILYTQAAVEERYGFSPKLLVDYKGLRGDPSDNIIGIKGVGEKTATTLVATFGTLE
                     SLYKVLKKNPEQVKAAGVSDRMVELLKEHESEALFSKTLATIRRDVPITYTLPEQPFK
                     EMFDSERALALCAKFEFRSLIPRMKNLFASEQEKKNDPETIMIHADAANPLVREAAVA
                     LWVLHSDYTNPTLDDILLVTKKETLADAYAYICAELDKQGLCEVFEQIEKPIISLVEE
                     MEQNGVLIDREHFARLATEYQKTLARLETDIHALAGVVFNINSPKQMSEVLFTALALK
                     PKGKRKESGAYTTNAETLESLRDEHPIIPKILEYREYQKLLSTYILTILEKAGADNRL
                     HAKFLQNGTTTGRFSSVDPNLQNLPMRGEQGKTIRRGFIAPAGWSLLSCDYAQIELRC
                     LAILSQDEKLIASFQGNDDVHATVASYMFKIPVNEVTGDMRRKAKVINFVILYGMGVS
                     ALQKNLNTSRAEAQQFYNEYFATFPAVAAYLESSKEAARTVGYTKTYFGRKRSFPQIN
                     SKLPFLRALAERTAMNAPIQGTSADIIKLAIGFAAADIDAAGLADSVKLTMQIHDELV
                     YEVKEEVKEQAQTVITRAMEGVFERSWLGKKEAVPLRVSASFGRTLGDVK"
     gene            11652..12128
                     /locus_tag="VLB02_01960"
     CDS             11652..12128
                     /locus_tag="VLB02_01960"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HSE56829.1"
                     /translation="MQRPPEQPQTVHEVLARSYLWFLILCSAGLFFDLFFPLYISLPH
                     AQTLGTILLVGAPVLILWAQSASYRFAQERERRASVTEAMFMYGPYKFLRNPTHIGLT
                     LLIWGYAFVTSSELLFITTLAATLISNWYFRKHEVLLEEKYGEPYRRYRASVNTFM"
     gene            12134..12763
                     /locus_tag="VLB02_01965"
     CDS             12134..12763
                     /locus_tag="VLB02_01965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56830.1"
                     /translation="MLAVIIGADHALRKEKIDSLLLKEGIAASEVLVLDEAQATIGTL
                     LSYADAPLFGAPPRALRARYFLEGVLPEDAALFTALHEARTLVLLEEKSLSAPLKKII
                     EKIGATLFTVEQKKAESKETVFSIANALATGDRKKLWLEYQRLLEKNAPEALFGILLW
                     KVRQLATAGSAKEKYKTIYKILLEAQANSRRTRLPFELALERALLMLPK"
     gene            complement(12966..13499)
                     /locus_tag="VLB02_01970"
     CDS             complement(12966..13499)
                     /locus_tag="VLB02_01970"
                     /inference="COORDINATES: protein motif:HMM:NF014639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HSE56831.1"
                     /translation="MLALFNLLNAGTTMLLEVAAVVLVVAALLRASWLSWVRRNAQPI
                     LFILLLASTAGSLIYEYAFHFPPCDMCWYQRIFIYSSMILLARALMLKKNVLGEVLTL
                     LFFGLAFSLYHNVMDIFGLSSLSFCEGGPSGSLCLVRYVYEFGYITIPIMSLTVLVLS
                     IIVVFIARNPQGGFGKH"
     gene            complement(13505..13885)
                     /locus_tag="VLB02_01975"
     CDS             complement(13505..13885)
                     /locus_tag="VLB02_01975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56832.1"
                     /translation="MTKKILIGLGIAVVIIGFFVFLYQQSNKPGKLDTFATCIKDSGA
                     RFFGAWWCPHCQNQKARFGRSAKLLPYIECSTPDRKQIENCTTEGITGYPTWKFADGS
                     VEKTEMELAELAEKTQCVLPTDTK"
BASE COUNT         3861 a         3038 c         3310 g         3727 t
ORIGIN      
        1 tagttggtgt ccatgtacca gcttctttgg taaaccccca ggtaactgat ccaatagcac
       61 ccaaactgcc gtcgtgtttt gcaaatgcgt gcttaatttc ggcagacgct ttgtttcggt
      121 tcgcagtgag tgcttgcacc acaatcccca caccgccggg accataccct tcataggtaa
      181 cgtgatccaa ttctttttgt tcatttgctt ttgagattgc gcgctcgatg ttctcgttcg
      241 gcatgttttc agcgcgcgct ttctcgattg cagcgcgcag gttcggtgcg tttcggtctc
      301 cctttgctgc ttttgcttcg ttcccaatca acagtgcgag ttttgaaaac accttgctct
      361 tcttcgcatc actcgctgcc tttttatgtt ttatttgttt ccatttcgaa tgacctgcca
      421 tattatttct ttaaatattc ttttcctttc tccgtaatca ctcggccgcg gggggtgcgt
      481 tcgatgagcc caacctggat caggtacggc tcgatgacgt cttcaattgt cccttcatcc
      541 tcgccggtga gtgctgctaa atttttaata ccaaccggac cgccactaaa cttttcatca
      601 attgctttta aaatagtgcg gtcattttgc gagagcccca gtgcatcaag ccctaaaagt
      661 gcaaacgctt cttcaactgc actgctatca agttcacgct tatgaatctg cgcatagtcg
      721 cgcacacggc ggagcaagta gttagcaatg cgcggggtcg agcggctcct gccggcaatg
      781 tgcgagagca gttcgtcttt aatactaata cctaaaatgt ctcccgagcg gcgaaggata
      841 gtactaattt cgctcggtgt ataaaactcg agccgaaaag caccacctga aaatcgtgag
      901 cgcagcggcg aggagagcat cccaaagcgg gtagtggcac caacaagtgt aaatgcgggg
      961 agctcaagtg caatagtgcg tgctgccgga cctttgccga tcataatgtc gagcttgccg
     1021 gtttccatca ccggatagag cacctcttca atggttttat ttaagcggtg aatttcatcg
     1081 ataaataaaa tatctccagg agaaaggttg gtaagaatgg ctgcaagatc gccaacgcgc
     1141 tcaattgttg gaccagaagt agtttttagg tttgcgccag tttcacgggc aataacatgg
     1201 gcgagtgttg ttttgccgag cccgggagga ccataaaata gaatgtgctc gggcatgtgc
     1261 ccgcgctctt ttgctgcccg aatcaggatc gaaagatttt ttttaatatt ttcttgcccc
     1321 acgtattcgt caaaagtgtt cggccgcagg gttgcgtctg agaactgctc gtcgccagat
     1381 gaagagaggg tgtttggtgt tatacttgac atagtaattt tagtatgcta tattctttcc
     1441 agaatgaatc tgagcaatta gattttacct taagaaggca ctaatctcta agcagccagc
     1501 ctgcgggttc taggggcgtg gataggacgt tctggtttcg actggggcca tcctacgatc
     1561 actctttact tatacttact attatttata agggctgttt agagattagt gcttttttga
     1621 atcatttact tttaaaataa acaacatgat aaaagttttt tataagttac ttatcggaaa
     1681 tactagtttt actaaagttt gtaaagtcca ctctgtccca gctgaaggaa cgttgctaaa
     1741 agcagcaaat ttttctttta aggttttcga aattgaacag gatcttgatg tgtatattag
     1801 tgatggagtt gaactgggtc aattttctga ttcagtaaaa gcaaatttta acgatgaatg
     1861 ggaaatgcct agtggaagta ggttaaaaaa actttttgaa gatttaacta aggctggatg
     1921 ggagcaaaaa taaccagtcc taaaaactgc actcagggtg cagttttttt aatcctccgt
     1981 aatatccacc acactcatca cctcggggaa gtcggtgatg ccttttaata tttttaccag
     2041 cccgtcttcc ttcatatcca aaattccctg ggcgagagat gcttttttaa tatcgcgctc
     2101 ggtggggctc gccataatta ttttttcgat cgcttcgtca gttaatattg cttcgaatat
     2161 tcccactcgc cctttgtagc cagtgttgtt gcagtgcggg cagccttttg cgctccacag
     2221 tttggtaacc tcgcgcgata cgctgcgtgc acttaagtcc tttccttcag ctgcagcttt
     2281 ttcccaaatg gtattgatgc gcactttttc ttctgcggtt acctcgcgct cgtacttgca
     2341 ctcagtacac agcttgcggc acaggcgctg ggcgatcgag cagccgagcg ctgacacaag
     2401 gatttttggg ttcacatcca ggtcaatcaa tcgcggaatc acgccagaag cattgttagt
     2461 gtggagtgtc gagaatacga ggtgtccggt gagtgcgctt tctacagcga tttttgctgt
     2521 ttcggggtcg cggatttcac ccaccatgat tacatcgggg tcttggcgga gcgcactgcg
     2581 gagcccttct aaaaaggtgt aacctttttt gtggtcggtc tgtgtttggg tgatacccgg
     2641 caggtggtac tcaattgggt cttcaatggt aatcacttta atttcaggtg tgtaaatttt
     2701 ttgcaaaaaa gcgtagaggg tagtggtttt tccggagcca gttggaccag tcagtaaaat
     2761 catcccgttc ggcttcttta tctcgcgcat aaaaatctcg tagagctctg gagaaatacc
     2821 gagttcgtcg aggttcacct gaattgattt tggattcaaa atacggagca cgattgattc
     2881 gccgtaggcg ccggggatca gtgaagtacg gtagtttatt tcagcttcat tcataaagct
     2941 cgagaagcgc ccgtcctggg gaacttcttt gacggtgagt tttaatcccg agagcagctt
     3001 tacgcgtgag tttaacagct gatagaggtg tgcatcaaaa ttcataatat tgtagagtac
     3061 cccgtcaagc cggtatcgca ggcggataaa atcagcttca ggctcaatgt gcacatcaga
     3121 ggcgccgagt gcaatacctg ctcctaaaat agtttcgagg atgcgcgata tggcatgggt
     3181 ctcttttgac tggattgcct gggtaataaa acgtatggca tcatcggttg tttttacccg
     3241 ctctgccatt tcaacaagta catcctgcga aatatctaat ccgccggcgc gggtggtggt
     3301 ggcgtaggaa agatctttat atcggctcca taccttttca atactttttc ggctcgccat
     3361 aaaaaaactt accgcgtagc cgcgtgattc ggcgatggtg cgtgcttgca gagcaccgtc
     3421 tgaggtaggg gaatgcacgg cgagtgagag ttttttgccc actaagttaa atggtcctac
     3481 ctcaaactgc cgcgattcag tttcgcttaa aatgcggagc gcatcgttat cgattgaggt
     3541 gctggttaaa tcgatgtacg gaacatgaaa ctgtgtttca gccagcatct ggagcagatt
     3601 ctcttcttca tcgcgcttga gctcatcgag ccgcttattc tgcttttctt catcaaatga
     3661 gagtggcatt gcgtttgtaa gtataccgca gaagccccat acacacgctg aaataagaag
     3721 aaaatcctat tgacaaaaaa caatttttgt tatagggtga gaaaaaattc gttgaaacaa
     3781 aaaacactct atatgaatct aaaaaagata aatcttaaaa aaagtgatag agagaaaatc
     3841 tattacgctt ggtttgtttt tgtgatagct atggttgttt ggtatctacg gacaccatat
     3901 ccctttttgg agttttggtc cgaaccggta catttattta catttttttc tggatctatt
     3961 gggctaatgc tgacttttgt aatggtcgtt agcgcctaca gaaacaaacg ggagatagaa
     4021 aaaaagatac cagtttctgc acagttagtt cctgattcag aaaaaaaacc agaaactacg
     4081 ccgacggaaa cacccatttc gacaaaaaaa cgaatcagta cccaattttg ggagactgtt
     4141 tggtttaaaa atcttgtata tggggttggt gtagctattc ttatcatact gtccatatgg
     4201 ttcgcctact ctaatcgtga atggattaaa agtaacctcc tctaccttct tgctggattg
     4261 gtatttattt tcttctgtat ttatgctcag cagatacttg caattccgag tataacaaac
     4321 aggaataagc aaactaatct tgttcggttg gtcgtagtgt gtctcgttgt ttcgcttatt
     4381 gtgaatgggg taaacttttt ctttcccagt ttttctagcg caggtaaaat ttacatagaa
     4441 aaccccatta acccgaaagt aaaacgagag agagattcac tagaggctga gaatacacgg
     4501 aaagatttat tactcgctac acagaagggg gcagtgactg ctcgcgaaca agaactgcag
     4561 gtggttaagc agcaggaaga ttcagtacgt aagaaaaatg ctgaattttc tcgaatgctt
     4621 gctgctcaaa atgacagcat cctcttccta aaagagcgga gtgttggcta tgctgatatg
     4681 caaaaaaagc tcgttgttgc tgaaacaaaa gaagattccc tcgtcagaga agttactagt
     4741 gttaatgtac aacttgtcaa agcagcaggg gagcagaaaa aactacagca acaaaattca
     4801 tttttaggaa gtatcttaga ttctacaaaa aaagctgata gtacaaaatt tgttgcattg
     4861 caacagcagc ttgcggagcg taataaagag acaggaaaca ctactacagc ttcagttaat
     4921 ggattaggta aagggatact ggttgtaata ggaataagta ttgtcattac aggaatactt
     4981 atcttctcat gggctaagtt tgggcaaacc agaaaaaaac agttgcttat tggattagca
     5041 ggctcagcaa ttataattgt gctcacgctc acttttagtt ggttcttttt ctctaaagag
     5101 cctgaagttg ttcacgcgga agagcaggaa aaacaggagt tgaaagatag tcttgccaaa
     5161 gaatatcaag ttaaacttgt agatactttg aaaaaagttg caaaacttcc ggttccggtt
     5221 gttcatgcct cagataccgc actcgctaca agtttagaac gtgagagggt cttgaaagaa
     5281 tctcttgctg ctaaagctga ttcacttttt gctgttaaca aggaaaaaaa cgccattatt
     5341 gattcgttga agcttgttac taaaagagat aacattgcgg atacgaccca gcagcgttca
     5401 agatcttctg tggtaataaa aggtccaggg aggtatagca tccccagaaa tcaggttgtt
     5461 gtacgatcaa agaagaggaa tccagggaaa tatagcttac cttaaggata ttaagaatat
     5521 agttagttac taatggcagg agaaatcctg ccattttttc atacctaagc gcacaaggaa
     5581 gtggtattga catattctat tatatatgct actctaaacg cagaaccaaa aaattattaa
     5641 aaaacaattt ttcacccata aaaacttatg taatgaaaaa gttattcagt tccttgatta
     5701 tttttactgt ttttacagtt accgcgcagg attacctgaa tggtacccct gacggtgctg
     5761 gatttctccg aaaatcggag tatggaaagc atatggatga agaagaagtt caatcctttt
     5821 ctacatcggg aaatacactt tcaatctcga accttaaagg ttcgaaagcg aaagagtata
     5881 tttcagcaca cacgctccag tttctgggag aatcatctac gcaagccgct tttggaaact
     5941 ccaaagtaat tggtgttggc gaacttcctg cagggacaaa aatatccact atggatatgc
     6001 atggaaatca tatacttcgg gatccttatc ccggagaaag gttttatttc catacatcca
     6061 caggaaaggt ctggttttcc ttgtactgct ggaatccggt gtttgtaacg attacccaac
     6121 cagctccaac gaataataat tatgttggcg gtggtaacaa ggaatttaaa gaagaaaaaa
     6181 cttcttgtga aatttcctgg aaaaccgggt atgccattta ttcgcaaggt cgcaatgatc
     6241 gcgaaggtga catgttggtt gatgccagtc tttacacccg cattcaggca gtgaatcagt
     6301 gttgtggagg tggaggaaat aacggcggtg ggtcaggggc gtctgtttac aaccaagcat
     6361 ctgaaactgt acggtatgta caggttcaga atggaggtgg aggaaatact tttggatcaa
     6421 ctctccttgg ctccgccata ggaacatttg ccggacacgt tgttggtgaa ttacttatgg
     6481 atggaagagg tcagccggtc attgttaatc aacgtcaagg ggtagtcctt acgagcgata
     6541 atggcggcgt tggaggtgga gggaattatc caagcacgtt tactccatgg cacgctggca
     6601 ccggtgatgg ctcaagagga aatttaccca gaggatactt taggtaaaaa attgtttaaa
     6661 gtaaaaaaaa cgaaaggtgg ttaagcacca cctttctatt gtttagtgcc gtacgtggaa
     6721 atagggcatt aaacagtagt attgacttat atcatttata tgatataatg aagctgttaa
     6781 attaattaag tggaattcaa gagaaagagt atgaaaaaaa ttattatttc aatgtttgcg
     6841 ctttttggga tgcttatcgc tacccctatt ttggcacatg cagctgatcg gtttaatact
     6901 gatccgaacg accttccaac tatcatggta accaacgtta cccaggatgg tggttgcagt
     6961 gcggggacag gggctgggtg ctggaagact tcggtagatg cacgcccggg ggacacggtt
     7021 gctgttcagg tttactttca taatgctact aatacaatag cgtatgatac acacctgagc
     7081 cttcagcctg gagagacatc tcgtggtact acgcatacct ttagtggcgg tgtagcatca
     7141 tcttctattg atcgagcaac tggttctgca acggtgcgca catcgagtac ccagaaactt
     7201 acctttatca atggttcggt tcgtgtattt aggcacggcg accaggttgg gttttcagta
     7261 gacaacgaaa attcactctt tggtactgga ctttcaattg atgaagttgg cgtcggtgtt
     7321 caaggggtac ttatggcaaa gtttaaagtt tcaaatgatg ccgttactga tgacgataat
     7381 tgtcggatta ctgattttac tgccgatgat tatacgatcg atgaagacga atcaactcgc
     7441 cttcgatggg ataccactga ttgtgactat gtaaaaatta gtcacatcag cggcaacctt
     7501 gatactgatg gcagtgtttc agtgagtcca aatgaggata caaagtatat tcttactgcg
     7561 tacccgggcg gcgatactga agaccttacc attcgtgtga acgaagatag caacaataac
     7621 aattgtgata tctcattcta tgctgatcgc accagcatta ctcgcgggca gtcaacaaag
     7681 ctgcactggg atgtaagcga tggaaacgat attgatattt caaatctcga ttcaaatgtt
     7741 aatgagtctg gcagtaaaac ggtaagccca aactcgacta cgacctaccg tcttacggtt
     7801 aatggcggtg attgcgataa tgaatcaaag tcagtaacta ttcgcgttga ggatggtgca
     7861 agcacggcaa agccgcaggc gattactaac ccagcaacac tcgttgctgc aaccgcagca
     7921 aagttaaatg gtattgcgat tccaaatgca aacgtgaaca ctagcgcttg gtttgaatgg
     7981 ggtccaacgc cagctcttgg tgctgcaaca gcagcccagg gtgtaggttc agcaggctct
     8041 ctcgcgtatt catacacggt tggcagcctc atgccaaata ccacgtacta cttccgcgct
     8101 gtggtgcaaa atgcaaacgg tgtcgggctc ggcgaaacac tttcgtttcg tactggtgca
     8161 acggtaacta acacaactcg ggtagtacgg agtacgcggg tggtgagcag tgcagtaact
     8221 gcacgcagca ccgcctcgct ccttgagctc agtgttgaaa atgcctacga ccgcatgtgt
     8281 gcgggtggag aaattgaata cattgttacc tacaagaaca tcagtcgatc taccttggta
     8341 gacgcagtgc tccaggtaac gcacccaaag cagctcgtgt ttgttgacgc gactcgcgga
     8401 gactactcgg cgagcgagcg aaaactcacc gttgacctcg ggcagcttgc tgcaggcgag
     8461 gaaggctcaa tccgcgttcg cggacgggtg actgacaatt ctgttgttgg tgacctgatt
     8521 gttaccactg caacagtggt gtacaccaac ccaacaactc gtgctcagga agatgcaatt
     8581 gcgtactcac tgaccaccgt ttcagacgat tgtccaaacg gatttggtgc agcatcactc
     8641 ttcgggagcg gttcattcct tcccgataca cttatcgaat ggctccttct tatattggtt
     8701 atccttgcat taatgttgct tggacgaaac ctgtacaata agaaagcaag cgcataacga
     8761 aatgctcaaa aaccccacct cgtgagaggt ggggtttttg ttatgttaga atccccctat
     8821 gcaggagaga tttacattat ttgatattcc gcggattgcc cggcagctgc ttacagtgtt
     8881 gcgccctgtc gaggatcggg caactatcct tgcactttcg ggcgagctcg gggcagggaa
     8941 aaccgcgctc gcgcaagcga ttggtgcagc gcttggggta gcagagcaga tggtgagccc
     9001 cacctttgtg ttgatgaagc agtaccaaac tccggcagga ccatttacta ctattattca
     9061 tatcgatgca taccggattg aaaacgaagc agagcttttg ccaattcatt ttgaagcact
     9121 cacgcagcag gggaatacgc tcattattat tgagtggccc gagcatatcg ccgctgcgct
     9181 tgccggcagg gtggtgtttc ggtatacgct ttcgcacctt gaaagcgatg agcgaatgat
     9241 cgagcagttg ccatgaccaa gaaaaaaaac aaaacattgg tactcttaga tgcgcacgcg
     9301 attatccacc gcgcgtacca tgcgctcccc gcatttacga atactgccgg agaaccgacc
     9361 ggggcgctct acgggcttac caccatgctg cttcggatta ttaccgacct gaaacctgac
     9421 tatttagttg ctgcgtacga tctcccaaaa cccacctttc ggcaccaggc atacgaggcg
     9481 tacaaaggaa cgcgggtaaa agcagatgat gaactgaaag cgcagctcac acaatcgcgt
     9541 gaagtattcg atgcatttgg cattccgcat attgatgctg aaggatttga ggcggatgat
     9601 gtaatcggta ccctggttga gaaatttaaa aaagaaaaag atcttactat tattatcgct
     9661 tcgggtgata tggataccct gcagttggtt gaaggaaaca gggtgcaagt atttaccctt
     9721 aagaagggga ttaccgacac aatactatac acacaagcag ccgttgagga gcgctacgga
     9781 ttttcaccaa aactgcttgt tgactacaaa ggattgcgcg gggatccgtc ggacaacatt
     9841 atcggcataa aaggagtggg agagaaaaca gcgaccacat tagtggcaac ctttggtacc
     9901 cttgaatcgc tctacaaagt gctcaagaaa aatccggagc aggtaaaagc tgccggtgtt
     9961 tcggatcgca tggtcgagct cttaaaagaa catgaaagcg aggcgctgtt ttcaaaaaca
    10021 cttgccacga ttcgccgcga tgtaccgatt acctatacgc tgcccgagca gccgtttaaa
    10081 gaaatgtttg attcagagcg agcactcgca ctctgtgcca agtttgagtt tcggagtttg
    10141 attccgcgaa tgaagaattt gtttgcaagc gagcaagaga aaaaaaatga tcctgaaaca
    10201 ataatgatac acgctgatgc agcaaacccg ctggtgcgtg aagcagcagt ggcgctctgg
    10261 gtgctgcact ctgattacac taacccaaca cttgatgaca tactgctggt tacaaaaaaa
    10321 gaaacactcg ctgatgccta cgcatatatt tgtgctgaac tcgacaagca aggactatgc
    10381 gaagtgtttg aacaaattga aaaaccgatt atttcgctgg ttgaagagat ggagcagaat
    10441 ggggtgctga ttgatcgcga acactttgct cggcttgcaa ccgaatatca aaaaacactc
    10501 gcgagattag aaactgacat tcacgcactc gccggtgttg tgtttaatat caattcacca
    10561 aagcagatgt cggaagtgct gtttaccgca cttgcactga agccgaaagg gaagcgcaaa
    10621 gagagcggag cgtacactac caatgctgaa acactcgagt cgctccgcga tgagcacccg
    10681 atcattccaa aaattcttga gtatcgcgag tatcaaaagc tgctctcaac ctacattctt
    10741 actatccttg aaaaagctgg agctgataat cggctgcatg cgaaattttt acagaacggt
    10801 accactacgg ggcggttctc atcggttgat ccgaatttac aaaatttacc gatgcgcggc
    10861 gaacagggaa agactatccg tagaggattt attgcaccag cgggctggag cctgctctcg
    10921 tgcgactacg cccaaatcga gctgcgctgt ttggcgatac tttctcaaga tgaaaaattg
    10981 attgcctcat ttcagggcaa cgacgatgtg cacgcaaccg ttgcttcgta catgttcaag
    11041 attccagtga atgaggtaac gggcgacatg cgccggaagg cgaaggtaat caacttcgtc
    11101 atcctctacg gcatgggagt atcggcactc caaaaaaact taaataccag ccgggcagag
    11161 gcgcagcaat tttataacga atattttgcc acctttcctg cagttgccgc gtacctcgag
    11221 agctcaaaag aggcagcgcg aacagtaggc tacactaaaa cgtactttgg ccgaaagcgc
    11281 tcatttccac agatcaattc aaagctgcca tttttgcgcg cgctcgccga gcgaacagcg
    11341 atgaatgcgc ccattcaagg aaccagtgcc gacattatca agcttgcgat cgggtttgca
    11401 gctgccgaca tagatgccgc cggacttgca gattcagtaa agctcactat gcagatccac
    11461 gacgagctgg tgtatgaagt aaaagaggag gtgaaagaac aggcgcaaac agtaattacc
    11521 agagcgatgg agggtgtgtt tgaacgctcg tggcttggca aaaaagaagc agtaccactc
    11581 cgcgtttctg cttcgttcgg cagaacgttg ggcgatgtga agtaggcgga ttcttgtgat
    11641 acagtactgg tatgcaacgc cctccagaac agccgcagac cgtgcatgaa gtgctcgcac
    11701 gctcgtacct gtggtttctc atcctctgct ctgctggtct tttttttgat ctcttttttc
    11761 cgctctatat ttcactcccg catgcgcaaa cgcttggtac catactgctt gttggtgcgc
    11821 cagttttgat actctgggcg cagagtgcat cgtaccgatt tgcacaagag cgcgaacgga
    11881 gagcttcagt tacagaagcg atgtttatgt acggaccata caaatttttg cgcaatccta
    11941 cccacatcgg gctcacgctc ctcatctggg gctacgcgtt tgttacctcg agcgaacttc
    12001 tttttattac gacgcttgca gcaacactca tttcaaattg gtactttcgt aaacacgaag
    12061 tactgcttga agaaaaatac ggcgagccgt accgccgcta ccgtgcatca gttaacacct
    12121 tcatgtaagt tgtatgctcg ctgttattat tggtgctgat catgcactgc gaaaagaaaa
    12181 gatagattca cttcttttga aagaaggaat tgctgcaagt gaagtgttgg tgctcgatga
    12241 agcacaggcg acaattggca cactgctctc atatgccgac gcaccgctgt ttggggcacc
    12301 gccgcgcgca ctgcgtgccc gctatttttt agagggagta ctgccagaag atgctgctct
    12361 ttttacagca ctccacgaag cacgcacgct tgtactgctt gaagaaaaat cactcagcgc
    12421 tccgctaaaa aaaataatag aaaaaatcgg cgctactctt tttacggttg agcaaaaaaa
    12481 agctgaatca aaagaaaccg tgttctcaat tgcaaacgca cttgccaccg gcgacagaaa
    12541 aaaactctgg ctcgagtatc agcggctttt agagaagaat gcacccgaag cactctttgg
    12601 gattttgctg tggaaggttc gccagcttgc gaccgcagga agcgcgaaag aaaaatataa
    12661 aacaatctat aaaattttgc tcgaggctca agcaaactct cgccgaactc gattgccgtt
    12721 tgaactcgca cttgagcgag cactgctcat gcttccaaaa taaaaagtac aaaaagaaag
    12781 acccctcgga ggaagttagg ggcctttaat aatttaggag taaaaaatgt atatattttt
    12841 aatgaacttt cggaaagaac tactatgaag ataaagtaca ttcacaaggt actaatttgc
    12901 actgaaggta aaaaaccaaa acaaaagaac ttaaagcacc ttcaagtatc agtatacgta
    12961 aaaaactagt gtttaccaaa gcctccctgt ggatttctcg caataaatac cacgataata
    13021 ctgagtacga gcacggtgag cgacataatc ggtatggtta tatagccaaa ttcatatacg
    13081 tagcgcacta aacagagtga tccgctcggt ccaccctcac agaacgagag acttgaaagc
    13141 ccaaaaatat ccatcacatt atggtagagc gaaaaggcaa gtccgaagaa aaggagggtg
    13201 agtacttcgc ccagcacatt ctttttcaac ataagtgctc gagcaagcag gatcatgctc
    13261 gagtagatga agatgcgctg gtaccagcac atatcacagg gtgggaaatg aaatgcatat
    13321 tcatatataa gactgccagc agtggatgca agaagaagta taaataaaat tggctgcgca
    13381 ttcctgcgaa cccacgagag ccacgacgca cgcaggagtg ctgcgactac taatactact
    13441 gctgccacct caagcagcat ggtggttccg gcattgagca gattaaaaag tgcgagcatg
    13501 gtaattactt agtgtcagtc gggagtacgc actgtgtttt ttcggcgagc tcggcgagtt
    13561 ccatttcggt tttctctacc gagccgtcgg caaacttcca ggttgggtag ccggtaatgc
    13621 cttcagtggt gcaattttca atttgcttgc gatcaggagt tgagcactcg atgtagggga
    13681 gtagttttgc cgatcgacca aaccgcgctt tttgattttg acagtgcggg caccaccacg
    13741 cgccaaagaa tcgtgcgccc gaatctttaa tacacgtcgc gaaggtgtcg agctttcccg
    13801 gtttatttga ttgctggtag agaaacacga agaatccaat aatcactact gcaatgccga
    13861 gcccaatgag aattttttta gtcatacaac tgtagcctac cgtttaggtg cacttcctgc
    13921 aagtgccaaa aaattc
//
LOCUS       DATIBQ010000062         2394 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U16294
            k141_1509430, whole genome shotgun sequence.
ACCESSION   DATIBQ010000062 DATIBQ010000000
VERSION     DATIBQ010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935789
            Sequence Read Archive: SRR25158331
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2394)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2394)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:58:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 637
            CDSs (total)                      :: 603
            Genes (coding)                    :: 601
            CDSs (with protein)               :: 601
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2394
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1509430"
                     /isolate="SMAG_U16294"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..753
                     /locus_tag="VLB02_01980"
     CDS             <1..753
                     /locus_tag="VLB02_01980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56833.1"
                     /translation="IKSILADKCGIDKKQVGEYFIFEHVIPSIPQQDPAIIVRSLREE
                     LTNGKMSFVITEVVNRLGCQKTNAAGGGIKFSNKFWEVSKGKTNGQGNGGTDILPGPD
                     KGVIQKEVDDSKDKILVGTINIQSSNEDDLGEKEKEVVPEPEDEIPGQKVFNIDFVSK
                     SAARKFLLNALDSIPSFRLTPHKLAKAFSEALKKKGLSVSPNNASIKMENGKLTIPSI
                     EFDELEEMLLKIITALIGGEEDNHLEDLKKEE"
     gene            complement(798..1244)
                     /locus_tag="VLB02_01985"
     CDS             complement(798..1244)
                     /locus_tag="VLB02_01985"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015125068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HSE56834.1"
                     /translation="MLTEGTITPPFSLPNAEGDTVSSTDFSGQWTLLYFYPKDDTPGC
                     TKEACAFRDNFPFYEDIGITVVGISRDSVASHKKFKEKYNLPFELLSDTEGAVIETYG
                     AANGTSTKRISYLVDPNGIIAKAYEQVTPATHAETVLKDLKELQGK"
     gene            1301..1813
                     /locus_tag="VLB02_01990"
     CDS             1301..1813
                     /locus_tag="VLB02_01990"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011244059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HSE56835.1"
                     /translation="MKYIAVAIVVIVLGVLVGASVRSDKNAGLEPSTPPPVLAVTESP
                     ADQAKAPLPEQSKKKTMEKEGVTIQVLKEGTGPEIKVGSTAVVHYTGQLVDGKVFDSS
                     KPRGTPFEFALGAGMVIKGWDIGVAGMKVGEQRKLTIPPALAYGASGVPGAIPANATL
                     IFEVELLGIK"
     gene            1826..>2394
                     /locus_tag="VLB02_01995"
     CDS             1826..>2394
                     /locus_tag="VLB02_01995"
                     /inference="COORDINATES: protein motif:HMM:NF024923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HSE56836.1"
                     /translation="MHTIQNKWRQVRAWYGILSLRKKVFFWIGIAIGVLIIVSILKGK
                     PSTVQVETVRRQDLARTVLASGKVVSSTDLALSFQKGDIVRSVRVSVGEKVQSGQVLA
                     TLDASEESAALTKAKGGLLAAEAAYRKVLEGNSDKEITVAQIAFENARRKLLSSDLVA
                     TPESIESADEAPVLSGTYNGTEEGEYRLE"
BASE COUNT          717 a          430 c          602 g          645 t
ORIGIN      
        1 attaaatcaa tcctggcaga taaatgtgga attgataaaa aacaagtagg agagtatttc
       61 atattcgagc atgttattcc ttcgattcca caacaagacc ctgcaattat tgttcgcagt
      121 ttaagagagg agcttaccaa tggtaaaatg tcttttgtta ttacagaagt tgtgaatcga
      181 ttaggttgtc aaaaaacaaa tgcagcggga ggaggtataa aattttctaa caaattctgg
      241 gaagtatcta aaggaaaaac aaacggtcag ggaaatggtg gaacagatat tcttcctggt
      301 cctgataagg gggttattca aaaagaggtt gatgacagta aggataaaat ccttgtcggc
      361 actataaaca tccagagtag caatgaagat gatttgggag aaaaagaaaa ggaggtagtg
      421 ccggaacctg aagacgaaat tcctggacaa aaagtattca acattgattt tgtgagcaaa
      481 agtgcagcaa ggaaattttt acttaatgct ctggattcaa ttccttcttt tcggctaaca
      541 ccgcacaagt tggcaaaagc attttctgaa gctttaaaga aaaaaggatt aagtgtttct
      601 ccaaacaatg cttccattaa aatggaaaac ggaaaattga ctataccttc aattgagttt
      661 gatgaattag aagaaatgct actgaaaata attacagcac ttattggtgg tgaagaagat
      721 aatcatcttg aagatttaaa aaaggaagaa tagttgaact attaaataaa gagcagttag
      781 taaatacctg ctctttttta ttttccctgt aattctttta aatctttcag tacggtttca
      841 gcatgggttg caggggttac ttgctcgtac gcttttgcaa ttattccgtt tggatctacg
      901 agatatgaaa tgcgctttgt tgaggttcca ttggctgctc cgtaggtttc aattactgct
      961 ccttcggtgt cagagagtag ctcaaacggc aggttgtatt tttctttaaa ttttttatga
     1021 ctcgcaacag aatcgcggct aatacctact acggttattc caatatcttc atagaaaggg
     1081 aagttgtccc gaaaagcgca ggcttctttg gtgcaacccg gagtgtcgtc tttggggtag
     1141 aagtagagga gtgtccactg tccggaaaag tcagtacttg aaacagtatc tccttctgca
     1201 ttggggagtg aaaacggcgg tgtgattgtt ccttcggtaa gcatagtaaa agtgtagcat
     1261 gctcgctgat tttatatttt ttggtatact ggtcggacgt atgaaatata tcgcagttgc
     1321 aattgtagtt attgtgcttg gagtgctggt cggagcgtcg gttcggtcag ataaaaatgc
     1381 agggcttgag ccgtcaacac cgccgccagt gttagcagtt actgaaagtc ctgctgatca
     1441 ggcaaaggcg ccattaccag aacaatcaaa gaaaaaaact atggaaaaag aaggagttac
     1501 tatacaagtc ttaaaagaag gaactggtcc tgaaattaaa gtaggcagca ccgcagtggt
     1561 gcactacacc gggcagcttg ttgatggaaa agtgtttgat tcaagcaagc cgcgcggtac
     1621 gccgtttgaa tttgcgctcg gtgccggcat ggttattaag ggctgggaca ttggtgtagc
     1681 agggatgaag gtgggagaac agcgaaaact caccattcca ccggcgcttg cctacggagc
     1741 gagcggtgtt ccgggagcga taccggcaaa cgcgacactt atttttgaag ttgaactctt
     1801 agggattaag tagtagggta cacccatgca tacgatacaa aacaaatggc gccaggtacg
     1861 agcctggtat ggtattctct cgctccgaaa aaaggtgttt ttttggattg gtattgcgat
     1921 cggtgtgttg attattgttt caatcctgaa aggaaagcca agtacggtgc aggttgaaac
     1981 ggtgcgccgg caagatcttg ctcgtacggt acttgcctcg ggaaaggtgg taagttcaac
     2041 cgacctcgcg ctctcgttcc aaaaaggaga cattgtccga tcggtccgtg tttcagtcgg
     2101 agaaaaagtg cagtcggggc aggtgctggc aacactcgat gcgtctgaag aatcagcagc
     2161 acttaccaaa gcaaaaggcg ggcttttggc tgccgaggca gcataccgaa aggtgttgga
     2221 aggaaattca gacaaggaaa ttaccgtggc gcaaatcgca tttgaaaatg ctcgccgaaa
     2281 actgctctca agcgatttag tggctactcc tgaatcaatc gaaagtgctg atgaggcacc
     2341 tgtgctcagt ggtacctata acggaactga agagggtgaa tatcgacttg aatt
//
LOCUS       DATIBQ010000063        10800 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U16294
            k141_867269, whole genome shotgun sequence.
ACCESSION   DATIBQ010000063 DATIBQ010000000
VERSION     DATIBQ010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935789
            Sequence Read Archive: SRR25158331
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 10800)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10800)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:58:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 637
            CDSs (total)                      :: 603
            Genes (coding)                    :: 601
            CDSs (with protein)               :: 601
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10800
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_867269"
                     /isolate="SMAG_U16294"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3862
                     /locus_tag="VLB02_02000"
     CDS             <1..3862
                     /locus_tag="VLB02_02000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HSE56837.1"
                     /translation="DATDHFGAPIGTFPTATFTAPLSINTNILTDVPGQDAQCITYDN
                     LPIGRYYYAPEEISNPAAWETPRYYDRGNTPQPSTDLTPYSGELFDEDNSNDVGIDDD
                     QSDGNVLLTNSQKFKTIIILNQMKKQEEGGGDDEDDTATISATKIVCDDESYLPNWGL
                     DSDNPTVTATTAADFLAAGNNAAHCHLQKDWKFEWAPSIEVNPGNNLEAAGGAWTASG
                     VTAADGTVSITVPVSQELVWFREQLKPDYIPFTGQTTDLENPLPSAEIHCNTDSLNYD
                     NYEWIAPLEAGSTYHCVAFNVPVKKTASVNLCKVDEQQVPLAGWTLLLKGESVQEGLA
                     VPTNLSAGIDSNPLAAGVSYIADVVGTWLNQGGANPADAEYSTIDGWTTQMDGYTGYQ
                     DDILELQIANTSGNWGTYTASHAYARSFTPALDGGVNFRIFDGEGTTQYDGTNSTPDW
                     FGDNLGSLLVNIAKGHSGITGENGCVQLSDVAYGDYSVDEVMKDGWQNVSGLGAVTVD
                     EPEETFTVVNKPVEQPKEDATIVATKIVCESETDLPNWGATSNDPLITATTAADFLAT
                     HPNCHLQKDWKFEWAPEGTANPGDNIIGTAGAPWTESGVTAVDGTVSISVPSGSDNLI
                     WVREQMQPGYIPFTGSADDASNVSAELHCNTDGLHYDNVEWISPRAPGETYYCVGFNV
                     PKKATLSVTKIVCPSEADLPNQGLEGGPLVTNTTAAEFLTAHPTCSLQPDWSFEWAPN
                     GTPNPGDNIIGAAGAPWSILGVTDGTGTFTAEVPVGDSLVWVRENLKDGYIPFTGVGP
                     DVSNVSAEFTCHTDGLHYDNYEWIGALEEGKTYHCVAYNVPKEQPPVDICPNIQGVQT
                     ILPDGYVLNEQGQCVPRGGGEGEDTEETIVVDSNNQNGWVFMDDNNSSILPDDYVAGP
                     GTAPFGDGSAHLAAAVVSDRDMLIKGFTGFNLDDITELSYRTYQASTSGAPAIALQFN
                     IDDDTTDLDNSWKGRLVYEPYYTHTIQVGSWQTWNTMDNSTNGSGDGNWWFSNGTLAT
                     NTGCAIATPCTWAEVLAALPNAGLHSAFGAVLFKVGGGWTGFDGNIDGFIIGIQTGIN
                     TATTTYDFEPLPPEPLAACANGVDDDSDGKIDFGEAEGNDPGCSSAEDDDETNAVPTF
                     TSTSNENNSNSGGSSGSVAHFSSFLPIPQVLGASTEACEEYIKTYIKLGAQNDSGDVT
                     RLQTFLNEHLGLSLIIDGIYGLQSFNAVKVFQKKYLNEVLTPWVPFEGPSVENGTGYV
                     YKTTKRWINMLKCPDLNLPVPQLP"
     gene            3953..4945
                     /locus_tag="VLB02_02005"
     CDS             3953..4945
                     /locus_tag="VLB02_02005"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HSE56838.1"
                     /translation="MKTVIKKAHSIIAPTKANGFIPYLLKDWSVWMVLIVAGGLFGFS
                     QLSDMTDTFGLTALVNPPTVVTLTNDARVQNQLPMLTTNPVLEAAAKLKAQDMASNSY
                     FAHTSPTGVTPWYWFRQAGYNFRYAGENLAVHYTESADVENAWLNSPTHRANIMNSNF
                     TEIGVATANGVYQGYPTTFVVELFGTPVAQKEPVAVQEKKIQPSSSKRVAESVQPQVA
                     GETAEAVPVEVLAESDTFVAVRNTDTPQNISWYQRLLLRSDEFIGLLFQAAIILLLFA
                     TGGMVLREYERHHKKHMAYGILLMVILGSMLYIGKLGIFQKTETFAAISQKTFK"
     gene            complement(4979..5467)
                     /locus_tag="VLB02_02010"
     CDS             complement(4979..5467)
                     /locus_tag="VLB02_02010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56839.1"
                     /translation="MGNALKSIIMNGLHINNPDKLEYFDFAQIAFDENGKNLLSPFKA
                     EKGKQYFLVIVVKKENEALKKYNALEGDIAQNALYDLKDGKMPNMKVPRALRSGPPQP
                     RPDEFISVPRFKGGEIIYYWKKGEEDRQFKIITLVNTLKTKMQFDVPREKQIYSIECI
                     TV"
     gene            5546..6103
                     /locus_tag="VLB02_02015"
     CDS             5546..6103
                     /locus_tag="VLB02_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HSE56840.1"
                     /translation="MSPLIIVLIIIGVLVIWFIAAYNGFVRMANRVKEAWADIEVQLK
                     RRYDLIPNLVNTVKGYATHESSAFEKVTQARAAAMGAKTVDQHAKTEAMLGSAITGLF
                     GIAEAYPDLKANTNFLELQRELSDTENKIQAARRFYNGNVRDFNTKLQTFPSNIVGNM
                     FGFKQSEFFDIPDDGVESKPVEVKF"
     gene            6131..6604
                     /locus_tag="VLB02_02020"
     CDS             6131..6604
                     /locus_tag="VLB02_02020"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HSE56841.1"
                     /translation="MEPLDKELHRIAVTTIVYRDDGKFLITRRSLEKKAFPGKWTVPG
                     GGLTTDDYVGTPPTHRNGQWYNTLENTIRREVKEEVGIEIGKPEYLLDITFIRSDGVP
                     VLILSYYAPFVRGEVTHDADTIDSAWVTAEEAKQYDLIDGIAEEIQMVDDILKKK"
     gene            6614..7504
                     /locus_tag="VLB02_02025"
     CDS             6614..7504
                     /locus_tag="VLB02_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019907485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HSE56842.1"
                     /translation="MATLYTQQHKNIRKTWFLMATFLIIVVAFGFIFSQIYNNPTILY
                     FTVLFSIFMNLFSYWNSDKIALSVSGAKPAGELEYIELHRIVENLSITAGLPKPKVYV
                     IDDPAPNAFATGRNSKHAAVAVTTGLLQVLDRSELEGVLAHELSHIGNRDILVSTVAV
                     VLAGFISILADMFLRGTFFGGGRSDREGKGNILMIIGIVLAILAPIFATLVQLAISRK
                     REYLADASGALLTRYPEGLARALEKIGAYARPMIRKSHATSHLFISNPFGGAGKKAAS
                     LFATHPPIEDRIKVLRDMSI"
     gene            7544..8410
                     /locus_tag="VLB02_02030"
     CDS             7544..8410
                     /locus_tag="VLB02_02030"
                     /inference="COORDINATES: protein motif:HMM:NF018406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 family protein"
                     /protein_id="HSE56843.1"
                     /translation="MKQFFHTVKASFGASSLYKQATVESGWKGIKFLVKFSLFLGLIL
                     GVVGIILSLIFVPQLTRKLSTFANNSYPDDLVLTFANKTLTTNRAEPVVVPLPPQFRE
                     ADTKELNLVVVAPTEPADPSVLEKYQAFAVLTSKGLVAGSARDQIRIQPYTSNAVVTK
                     ALFMSRAATILRMIRIAAVVGSIPLAIFFVLMIAGMHLLWLVIVALIIFLQLKIRKLS
                     FSYKQAYKMGIYAIVPLMLIEIIATPLDMAGKLLSALVLVGITWIASRHWVFEHTPTE
                     IVVQPPEIEPGA"
     gene            8571..8657
                     /locus_tag="VLB02_02035"
     tRNA            8571..8657
                     /locus_tag="VLB02_02035"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8605..8607,aa:Ser,seq:gga)
     gene            8643..8909
                     /locus_tag="VLB02_02040"
     CDS             8643..8909
                     /locus_tag="VLB02_02040"
                     /inference="COORDINATES: protein motif:HMM:NF022337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HSE56844.1"
                     /translation="MSLPPHQRQWRTYRSRIDKKSVDFVLFDIETFTPHLVIELDDSS
                     HDREERHERDLFVDAVLKKAGIRIEYVKNAYSYDAKVLFTIVNL"
     gene            9010..9246
                     /locus_tag="VLB02_02045"
     CDS             9010..9246
                     /locus_tag="VLB02_02045"
                     /inference="COORDINATES: protein motif:HMM:NF021471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HSE56845.1"
                     /translation="MKKNIHTVFNSERKMWESKLEGQEKPVSSSHKKDTAMEKSIKEA
                     KERKVEHVIHNKDGIITDKDSYGKDPNPPKDAKH"
     gene            9251..10126
                     /locus_tag="VLB02_02050"
     CDS             9251..10126
                     /locus_tag="VLB02_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56846.1"
                     /translation="MSGKQDIVEGVDAIVTETTEGEIITGAEKEVAKRFAQGSNDKYG
                     RFFLAALSAIPWVIPLSLIASLKGEMDQDGLNSSFRLWLQEHQEKLKEVVQTINEILT
                     RLDSFGAEVQQRIESPEYLNLVRRAFRTWDEADTQEKRLMLKQLLTNAGAITLCPDDQ
                     VRLFIDWIERYHEAHFAVIKEVYLHQPITKGRIWDNLHSEGRPRDNTAQAGLFGYLTR
                     ELNMGGIIHLNRQTNAYGQTMRSPRQSSSGSNRSDTLESHFDDTKEWVLSELGKEFVR
                     YVMKDVDLQLQDAPN"
     gene            complement(10210..>10800)
                     /locus_tag="VLB02_02055"
     CDS             complement(10210..>10800)
                     /locus_tag="VLB02_02055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56847.1"
                     /translation="IATAFVLFSCNQKNETEKKTSVTIDTVSKATLAKRLPTRTAGEM
                     LMDSIYKNVVDSVNTFIDTYGDGRGLGDAWWDSKIPNDQPFNRIRLCKTIGDALIIER
                     RIFPFNNKGEVLGYMIDKEYFSDSTYRYFVESSRYMKNEEVKKDRKTEQITTPEEMAL
                     ATEVYRTRLKQAYDNLAILNGKQEKQKTIQTLSKNG"
BASE COUNT         3098 a         2317 c         2448 g         2937 t
ORIGIN      
        1 cgatgcaact gaccacttcg gtgccccaat tggtacattt cctacagcaa cgtttaccgc
       61 tccgctctcg ataaatacaa acatccttac ggatgttcct gggcaagatg cacagtgtat
      121 tacctacgat aatttaccaa ttggtcgcta ctactatgct cccgaagaaa taagtaatcc
      181 tgcagcgtgg gaaactcctc gctactatga tcgaggcaat acaccgcagc caagtaccga
      241 tcttactccc tacagcggcg aactctttga tgaagataat tcaaacgatg ttggtataga
      301 tgatgatcag tccgacggta atgtattact cacgaacagt cagaagttca aaacaatcat
      361 catccttaac cagatgaaaa aacaggaaga gggcggagga gatgatgaag atgacaccgc
      421 taccatttct gcaacaaaaa ttgtttgtga tgatgaatca tacttgccaa actgggggct
      481 cgatagtgat aacccaaccg taactgctac taccgcagct gattttcttg cagcaggcaa
      541 taacgcagca cactgtcacc tccaaaaaga ttggaagttt gagtgggcgc caagtatcga
      601 ggttaaccca gggaataatc ttgaagctgc tggcggcgca tggactgcat cgggtgtaac
      661 cgcagctgat ggtacggttt ctattactgt tccagtttct caagagttgg tatggttccg
      721 tgaacagctc aagcctgact atattccatt taccggacag actaccgatt tagaaaaccc
      781 gcttccttcg gcagaaattc actgcaacac cgatagttta aattacgata actacgaatg
      841 gatcgcaccg cttgaagcag gtagtaccta ccactgtgtc gcctttaatg ttccggtgaa
      901 aaagaccgct tcagtaaacc tctgtaaagt tgacgagcag caggtgccgc ttgcaggctg
      961 gacactgcta ttgaagggtg agagcgtgca agaagggctt gctgttccaa caaatctctc
     1021 tgctggtatt gattcaaacc cacttgctgc cggagtctct tacattgctg atgtagtcgg
     1081 tacctggctt aaccaaggcg gcgcaaatcc tgctgatgct gagtactcaa ccattgatgg
     1141 ttggacaacg cagatggatg gttacactgg ttatcaagat gatattcttg agctccaaat
     1201 tgcaaacacc tcgggtaact ggggtactta cactgcaagt catgcatacg ctcgctcgtt
     1261 tacgccagcg cttgatggcg gcgtaaactt ccgcatcttt gacggcgaag gtactaccca
     1321 gtacgacggc accaacagta ctcctgattg gtttggtgat aaccttggtt cactgcttgt
     1381 aaatattgca aaaggtcatt caggaatcac cggagaaaat ggctgcgtgc agctaagcga
     1441 tgttgcctat ggcgactata gcgttgatga agtaatgaaa gatggctggc agaatgtttc
     1501 tgggctcggt gcagtaacag tggatgagcc agaagaaacg tttacggtgg taaacaagcc
     1561 cgttgagcag ccaaaagaag acgcaacaat tgttgcaaca aaaattgtgt gtgagagcga
     1621 aacagatttg ccaaactggg gcgcaaccag caacgatccg ctcattaccg ctaccactgc
     1681 tgctgatttc ctcgcaacgc atcctaactg ccacctccaa aaagattgga agtttgagtg
     1741 ggcgccagag ggaactgcaa acccgggcga taacattatc ggcacagccg gagctccatg
     1801 gacagagtca ggtgtaaccg cagttgacgg cactgtttca atcagtgttc cttcagggag
     1861 cgacaacctc atctgggttc gcgagcagat gcagccaggc tacattccgt ttactggaag
     1921 cgccgatgat gcatcaaatg tttctgctga actgcactgc aacaccgacg ggctgcacta
     1981 cgacaacgtg gaatggatct caccacgcgc accaggcgaa acctactact gcgttggttt
     2041 taatgtacca aaaaaggcaa cactttcggt aactaaaatc gtgtgcccaa gtgaagcaga
     2101 tttgccaaac caagggcttg aaggtggtcc gctcgtaacg aatactactg ctgcagaatt
     2161 tcttactgca cacccaacct gttcgctgca gcctgattgg agctttgaat gggcgccaaa
     2221 cggtacccca aacccgggcg ataatatcat tggcgcagcc ggagctccct ggagtattct
     2281 tggcgtaact gatggcaccg gaacctttac ggcagaagtt cctgttggtg acagcctcgt
     2341 gtgggttcgt gaaaatctga aagacggcta catccccttt accggtgttg gtcctgatgt
     2401 ttcaaacgtt tctgcagaat ttacctgcca caccgacggt cttcactacg acaactacga
     2461 gtggatcggt gcgctcgagg aaggaaaaac ctaccactgc gttgcataca atgtgccaaa
     2521 agagcagccg cccgtagata tttgtcctaa catccagggg gttcaaacaa tacttccaga
     2581 tggatatgtg ctgaatgagc aaggacagtg tgttccgcga ggtggcggag aaggtgagga
     2641 tacagaagaa accatagttg ttgattctaa caaccagaac ggatgggtgt ttatggatga
     2701 taataattcc tcaatccttc ccgatgatta tgtagctggt cctggcactg ctccgtttgg
     2761 tgacggcagt gcacaccttg cagcagcagt cgtgagcgat cgagatatgc tcatcaaagg
     2821 gtttaccggt tttaacttag acgatattac tgaactttca tatcgtacct accaggcaag
     2881 cacgagtggt gcacccgcaa ttgcattgca gtttaatatc gatgatgaca ctaccgacct
     2941 tgataattca tggaaggggc gcctcgtgta tgagccatac tacacgcaca ccattcaggt
     3001 tggcagttgg caaacgtgga acacgatgga taactccacc aacggatcag gagacggcaa
     3061 ctggtggttt tcaaatggca ctcttgctac aaacactggc tgtgcaatag caacaccgtg
     3121 tacctgggca gaggtgctcg cagcgcttcc taatgcaggg ctccatagtg catttggagc
     3181 agtactattt aaggtgggtg gcggatggac tgggtttgat ggaaacattg acggttttat
     3241 tatcggtata caaacaggaa taaacactgc cactacaacc tacgattttg aaccactccc
     3301 tccagagcct ttagcggcat gtgctaatgg tgtagatgat gacagtgacg gaaagataga
     3361 ttttggtgaa gcagagggta acgaccctgg ttgttcaagt gctgaagatg acgacgaaac
     3421 aaatgctgtg ccaacattca ctagtactag taacgaaaat aattcaaata gtggcggttc
     3481 aagcggcagt gttgcgcact tctcttcttt tcttccaatc cctcaagttt taggtgcaag
     3541 tactgaagcg tgtgaagaat acattaaaac ctacatcaag cttggtgcac agaacgattc
     3601 aggcgatgta actcgactcc agacattttt aaatgaacac ctcgggctga gtcttattat
     3661 tgacggtatc tatggtctcc agagctttaa cgcggtgaag gtgtttcaga agaagtactt
     3721 gaatgaagtg ctcaccccgt gggttccctt cgaaggtccg agtgttgaaa atggcaccgg
     3781 ctacgtgtat aaaacaacga agcgctggat caacatgctc aagtgccctg acttgaacct
     3841 ccccgtgcca cagcttccgt aatagtaaac ccggcagtga gccttggtct cgcacggtag
     3901 tgcaagcggc tgtgccgcaa gaccaagatc tactgccggg aaaataaatc ctgtgaaaac
     3961 agtcataaaa aaagcacata gtattattgc tcccacaaag gcaaatggtt ttatcccgta
     4021 cctcttgaag gattggtcgg tatggatggt actgattgtt gcgggcggat tgtttggatt
     4081 ttcgcagctc tctgatatga ccgacacctt tgggcttact gcacttgtaa atccaccaac
     4141 ggtggtaacg cttaccaatg atgctcgagt tcaaaatcag ctcccaatgc ttaccaccaa
     4201 tcccgtgctt gaagctgcag caaaactaaa agcccaagac atggctagta atagctactt
     4261 tgcccacacg agccctacag gggttacgcc gtggtactgg tttcgccagg caggctacaa
     4321 ttttcgctac gctggcgaga acttggcggt gcactacact gaatctgccg atgtcgaaaa
     4381 tgcctggctc aactcgccaa cgcaccgggc aaatattatg aacagtaatt ttactgagat
     4441 cggtgttgct accgccaacg gtgtgtacca aggctatcca accacatttg ttgttgaact
     4501 ttttggtaca ccagtagcac aaaaagagcc ggtagcggta caagaaaaaa aaatccagcc
     4561 ttcatcatcg aagcgcgtag ctgagtctgt acagccgcaa gtagcaggag aaacagcaga
     4621 agcagtaccc gttgaagtac tcgccgaatc tgacactttt gtcgcagtca gaaacaccga
     4681 tactcctcaa aacattagtt ggtatcaacg gctcctgctg cgttctgatg aatttattgg
     4741 gcttctcttc caggcagcaa tcatcctcct cttatttgct acgggcggga tggtgctgcg
     4801 cgagtatgag cgccaccaca aaaagcacat ggcatacggt atcctcctta tggttatcct
     4861 cggttcgatg ctctacattg gcaagcttgg cattttccaa aaaaccgaaa cattcgcagc
     4921 tatttcccag aaaactttta aataaaaata cagctctgtt agagctgtat tgcactgatt
     4981 aaacagttat gcactcaata gagtaaattt gtttttcccg tggaacgtca aactgcattt
     5041 ttgtttttag tgtgttcact agggtaataa ttttaaattg tctgtcttct tcaccttttt
     5101 tccagtagta aattatttcc cctcctttga aacggggaac tgatataaat tcatcaggtc
     5161 ttggttgggg tgggccactt cgtagagctc tcggaacttt catatttggc atcttgccgt
     5221 ctttgagatc atataatgca ttttgagcga tatctccctc aagcgcatta tattttttta
     5281 aagcctcgtt ttcttttttt actacgatca ccaaaaagta ttgctttcct ttttcagctt
     5341 tgaatggaga aagtaaattt tttccatttt cgtcgaaagc aatttgggca aaatcaaaat
     5401 actctaattt atcagggtta ttaatgtgta aaccattcat gatgatagat tttaaagcgt
     5461 ttcccatatt atatatcaga tattataaaa gtcaatagat gcttaaaaaa gcaaaataag
     5521 cttttatgtt atatacttag cctatatgtc accactgatt atcgtgctca ttattattgg
     5581 cgttttggtt atttggttta ttgccgcata taacggcttt gttcgaatgg caaatcgggt
     5641 aaaggaagct tgggcagata ttgaagtgca gctcaagcgc cgctacgatt tgattccaaa
     5701 tttggtaaac accgtaaaag gctacgcaac ccatgaatcg agcgcgtttg aaaaagtaac
     5761 tcaggctcga gctgctgcta tgggcgcaaa aactgttgac cagcatgcaa agacagaagc
     5821 aatgctcggc agcgctatca ccgggctctt tggtattgcc gaagcgtacc cagacctaaa
     5881 ggcaaacaca aacttcctgg aactccagcg cgagctctct gatactgaga ataaaatcca
     5941 agcagcccgc cgcttctata acggcaatgt gcgtgacttc aacaccaagc ttcaaacctt
     6001 cccctcaaac attgtgggca acatgttcgg cttcaagcag agcgaattct ttgacattcc
     6061 cgatgatggg gtagagagca agccagttga ggtaaagttt taatttttta ctcacaaaat
     6121 aagtttaaat atggaaccgc tcgacaaaga actgcaccga atcgctgtta ctactattgt
     6181 ctaccgtgat gatggcaaat ttttgattac gcgtcggagt cttgaaaaga aagcatttcc
     6241 gggaaagtgg acggtgccgg gcggtgggct tactaccgat gattatgtgg gcactccgcc
     6301 aacacatcgt aatggtcagt ggtacaacac actcgaaaat acgattcggc gcgaggtgaa
     6361 agaagaagta ggaatagaaa ttggcaaacc tgaatatttg ctcgatatta cctttattcg
     6421 ctctgacggt gtaccggtgc ttattttaag ttactacgct ccatttgttc gtggagaagt
     6481 tactcatgat gctgatacta ttgattccgc ttgggtaact gctgaggaag caaaacagta
     6541 cgatttaatc gatggtattg ctgaagaaat acaaatggta gacgatattc tgaaaaagaa
     6601 gtaaaacata tatatggcga cactgtatac ccaacaacat aaaaatatcc gaaaaacctg
     6661 gtttctgatg gcgacatttt taattattgt tgttgcattc ggctttattt tttcccaaat
     6721 atataacaac cccaccatcc tgtattttac ggtgctcttt agtattttta tgaatctgtt
     6781 cagttactgg aactctgaca aaattgcact ctcggtttcc ggagcaaaac cagcggggga
     6841 attagagtat atagaactac accgcattgt tgagaatttg agtatcactg ctggattacc
     6901 aaaaccaaaa gtgtatgtaa tagatgaccc agcgccaaac gcctttgcaa cggggcgcaa
     6961 tagtaaacat gcagctgttg cggtaacgac agggctgctc caggtgctcg accggagcga
     7021 acttgaaggg gtgctcgcgc acgagctttc acacattggc aaccgcgaca ttttagtttc
     7081 taccgttgca gtggtgcttg cgggttttat ttcaatcttg gcagatatgt ttttgcgcgg
     7141 cacttttttt ggcggcgggc ggagtgatcg ggaagggaaa ggaaatattt taatgattat
     7201 tggtattgtg ctggcaattt tggcacctat ttttgcaact cttgtgcagc ttgccatttc
     7261 gcgtaaacgc gaatatttag ccgatgcttc gggtgcactg ctcactcgct accctgaagg
     7321 tttggcgcgc gcacttgaaa aaatcggtgc ttatgcccgc cctatgatcc gcaagagcca
     7381 cgcaacctca cacctcttta tttcaaatcc gttcggcggt gctggcaaga aggcagcaag
     7441 cctatttgca acccatccac ctattgaaga ccgcataaaa gtactgcgcg atatgtcaat
     7501 ttaagttgcg ttcatgaccc tctttgggta ccatgtacgc tatatgaaac aatttttcca
     7561 tacagtaaag gcatcgttcg gcgcaagtag tttatataag caggcaacgg tagagagtgg
     7621 ctggaagggg ataaaatttt tggttaaatt ttctctattt ctcgggctga ttcttggagt
     7681 ggttggaatt attctctcct tgatattcgt gccgcaactt acacgcaaac tgagcacctt
     7741 tgctaacaat tcgtatcctg atgacctcgt gcttacattt gccaataaaa cacttaccac
     7801 caatagagca gagccagttg ttgttccgct gccacctcag tttcgtgaag cggatactaa
     7861 agagctaaac ctggtcgttg ttgcaccaac agagcctgct gatccatcag tacttgaaaa
     7921 gtaccaggcg ttcgcagtgc ttacttccaa agggcttgtt gcggggtcag cccgtgatca
     7981 aattcgcatc caaccctata cgagcaatgc agtggtaaca aaagcactct ttatgagccg
     8041 tgctgcaacg atactgcgaa tgataagaat agcggctgtt gtcggcagta ttccgcttgc
     8101 aatattcttt gtgctgatga ttgcaggcat gcacctcttg tggcttgtta ttgttgcgct
     8161 cattattttc ctacagctaa aaattcgcaa gctttcgttc tcgtacaagc aggcgtataa
     8221 gatgggtatc tatgccattg ttccgctcat gcttatcgag attattgcaa caccgcttga
     8281 tatggctgga aaacttctca gcgcgctcgt gcttgttggt attacctgga tcgccagccg
     8341 ccactgggtg tttgaacaca ctccaaccga gatagttgtc cagcctcctg aaatagaacc
     8401 aggtgcgtag gttgcaaact tcctctgaag cgctaaaatc agttggatca aaataaattg
     8461 ttatgtattg gaattattgg atttcttcct ctgccagtgt tgccaacctt tcttggcagg
     8521 tattgaggaa gtaaaagcct gtgccgccgt agaaaccggc ggcacttttt ggaggaatgc
     8581 cagagcggtt gaatgggcag tcctggaaag actgtgtact agtaatagta ccgcgagttc
     8641 gaatctcgct tcctccgcac caacgacaat ggagaacata cagaagcagg attgataaga
     8701 agtcagtgga ctttgtactt tttgatatag aaacatttac accacattta gttattgagc
     8761 ttgatgatag ttctcatgat cgagaggaga gacatgagag agatctgttt gttgacgcag
     8821 tgctcaaaaa agcagggatt aggatagagt atgtgaaaaa tgcttattct tacgatgcga
     8881 aagtattatt tactattgtt aatttataaa aacagttgta tagtattagt gactttagga
     8941 ctactgtaat cataattgcg gttggtttat ctctagcgtc tcaagtttat tagctaaata
     9001 gaaattagaa tgaaaaagaa catacataca gtatttaatt cagaaagaaa gatgtgggaa
     9061 tcaaaattag agggacaaga aaaacctgtt tcatcttcac ataaaaaaga cactgcaatg
     9121 gaaaagtcta taaaagaagc aaaagaacga aaggttgaac atgtaatcca taataaagat
     9181 gggataatta cagataagga tagctatggc aaagacccaa atccaccaaa ggatgctaaa
     9241 cattaagaat atgtcaggaa aacaagatat tgttgaagga gtcgatgcaa tagtaacaga
     9301 aactaccgaa ggtgaaataa taacaggtgc agaaaaagaa gttgcgaaaa gatttgcaca
     9361 aggttccaat gataaatatg gacgtttttt cttagctgct ttaagcgcaa taccttgggt
     9421 aataccatta agtcttatcg ccagtttaaa aggagaaatg gatcaagacg gtttaaattc
     9481 atcatttaga ttatggttac aggagcatca agaaaaactt aaagaggttg tccaaacaat
     9541 aaatgaaatc cttacaagat tagacagttt tggtgcagag gtacaacaac gtattgaatc
     9601 tccagagtat ttaaatcttg ttcgtagagc gtttcggacc tgggacgaag cagatacgca
     9661 ggaaaaaagg ctaatgctaa agcaactctt aacaaatgca ggagcgatta ctctgtgtcc
     9721 agatgaccaa gtaagattat ttattgactg gatagaaaga taccatgaag cacattttgc
     9781 ggtaataaaa gaggtgtatc tccaccaacc aattactaag ggaagaatat gggataacct
     9841 acattcagaa ggtaggccaa gagataatac tgctcaagca ggattgtttg gttatctaac
     9901 aagagaatta aatatgggtg gaattatcca cttgaatcgt caaacaaatg catatggaca
     9961 aacaatgcgt tcaccaaggc aaagttcttc tgggtcaaat agaagtgata ctttagaatc
    10021 tcattttgat gatacaaaag agtgggttct aagtgaactt ggaaaggaat ttgtacggta
    10081 cgttatgaaa gatgttgacc ttcaactcca agatgctcct aattaattaa ttaattaatg
    10141 tcccaatatt gatcgtgatt aaataataaa aaaagcagga cttagtcctg cctctgtatc
    10201 gttgttgttt taaccatttt tactaagtgt ctgaatggtt ttttgctttt cctgcttgcc
    10261 gtttaagata gcaagattat catatgcctg ttttaagcgt gtacgataaa cttccgttgc
    10321 taatgccatt tcttccgggg ttgtaatttg ttcggttttc ctatcttttt taacttcctc
    10381 atttttcata tacctacttg attcaacaaa gtatctgtaa gtactgtcag agaagtattc
    10441 tttatcaatc atataaccaa gcacctcacc tttgttattg aaaggaaata tccttcgttc
    10501 aatgataagg gcatcaccga tagttttaca gagccggatc cggttaaagg gttgatcatt
    10561 aggaattttg ctatcccacc atgcatcacc taatccgcgg ccgtccccgt atgtatcaat
    10621 gaaggtatta acggaatcaa caacattttt gtaaattgaa tccattaaca tttccccggc
    10681 ggttcttgtt ggaagtcttt tagctaaggt agctttcgaa acggtatcaa tagtaacgct
    10741 ggtttttttc tcggtttcat ttttttgatt gcaagaaaat aaaacgaatg cggtagcaat
//
LOCUS       DATIBQ010000064         2906 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U16294
            k141_2399192, whole genome shotgun sequence.
ACCESSION   DATIBQ010000064 DATIBQ010000000
VERSION     DATIBQ010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935789
            Sequence Read Archive: SRR25158331
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2906)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2906)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:58:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 637
            CDSs (total)                      :: 603
            Genes (coding)                    :: 601
            CDSs (with protein)               :: 601
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2906
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2399192"
                     /isolate="SMAG_U16294"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            139..1326
                     /locus_tag="VLB02_02060"
     CDS             139..1326
                     /locus_tag="VLB02_02060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56848.1"
                     /translation="MEQPHFELPLKPEKGASRHEPIKPLEEKEDEPIAYAAIAKEFYE
                     AIRRRAEVQAKESVRKVQAGKSPEHRTLYLPEVHFQNRLIYLLYQKIHDASFSFETAT
                     SRLKDELSRLIEKDKKLTDHDREQKQSTLCDDLEKMMRGIESVITVVESRSEVKEAAE
                     EYFIGTSDVLDATKKIDLVEYARTGEEAAIRFIQVKTGTVRKEDIENIAHVHQAYLDS
                     FLPAIQEIVHKDYDTQFKELLEQQGETFTADELAELQKKWNVISMAIAEAGTSAPSAK
                     ELCAYIEKNIVGYSNPVSIDDILFFREIVLSKELSQEVAELLDHDFLPVEGQAGLTSY
                     LQTMEEDFEELLERYLQKEGTLDIPTVSFYSTIATPIGTVQERALVNELKRTKPAVKL
                     TGR"
     gene            1362..1487
                     /locus_tag="VLB02_02065"
     CDS             1362..1487
                     /locus_tag="VLB02_02065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56849.1"
                     /translation="MMKVFKKKGAALKKERFPLSVKVAITRLRNTVAKKNGKQKK"
     gene            1569..>2906
                     /locus_tag="VLB02_02070"
     CDS             1569..>2906
                     /locus_tag="VLB02_02070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56850.1"
                     /translation="MQPTEKFETPKQEIDWIGKTAQERIIQQAQDERAPNGTDATKKT
                     IEAYAKAEAKEFLHETAILPTHETEHLVLKLKPEEHDIKIEELFKVALEKGIKNALDV
                     AAKFGDPHVEDDFHRFLVQYLHTIGAIPGLKEKSTLHGELDHSLFTVTLPSMQGESEK
                     QNFKTFIQAMKQFYQSMLALEKGAHYSLELAVQNHGRDIALYASVPRHGIDIFEKTVH
                     AYYPGASVEPAADDYNIFTVGGAIAGAYAQLAEPAIFPLNTDAAFEHDPLAALIGSLS
                     RLETAGEGAAIQIIIAADESKMNKVFESVITSAQKQPGEKVKDLYKEATGSILAGTGK
                     ALFSLFSDGKKDEEKKEKPVDTHGIELATAKNKSQTINAAVRVIASAQTPDRAASIIT
                     TVKSSFNQFSLVGSNSIEWKDIKTSHLFGFCQAFSFRSRNPDEELLLSIDELAT"
BASE COUNT          987 a          648 c          636 g          635 t
ORIGIN      
        1 aaaaagaaaa accaacagga gaaacggtgc aagaagtagc taaaaaataa attattacaa
       61 ttacaaaacc tgctcaatta ttaagcaggt tttgtaatac ctgttctcaa aaaaatattt
      121 ctctgctata actagcccat ggaacaaccg cattttgaat tgccactcaa accagagaag
      181 ggtgctagca gacacgagcc aatcaagccg ctggaagaaa aagaagacga accaattgca
      241 tatgcagcga ttgccaaaga attttatgag gcaatacggc gacgcgctga ggtgcaagca
      301 aaagaatcag tacgaaaagt acaggcagga aaatcacccg aacatcgaac actgtattta
      361 ccagaggtgc attttcagaa caggctcatt tatcttctat atcaaaaaat acatgatgca
      421 tctttttcat ttgaaacagc gaccagtaga ttaaaagatg aactgagcag attgattgaa
      481 aaagataaaa aacttaccga tcatgatcga gagcagaaac aaagtaccct ctgtgacgat
      541 cttgaaaaaa tgatgcgtgg tatagaaagt gttattactg tggtagagag ccggagcgag
      601 gtaaaagaag ctgcagaaga atactttatt ggcaccagtg atgtgctcga tgcgacaaag
      661 aaaatagatt tagtggaata tgctcgaaca ggtgaagaag ctgcgattcg ttttattcaa
      721 gtgaaaacag gaacagtaag aaaagaagac atcgagaaca ttgctcatgt acatcaagca
      781 tatctcgact catttcttcc cgctattcaa gaaatcgtac ataaagacta cgacacccaa
      841 tttaaggaac tcttggagca gcaaggagaa acatttactg ctgatgaact agcagagctg
      901 cagaaaaaat ggaatgtgat tagtatggca atagcagaag cgggtaccag tgcaccgagt
      961 gctaaagaat tatgcgcata cattgaaaag aacattgtcg gctacagcaa ccctgtctct
     1021 attgacgata ttttattctt tagagaaatc gttctctcaa aagagctctc ccaggaagta
     1081 gcagaattgc tcgatcatga tttcctccct gtagaaggac aggcagggct cacgagctac
     1141 ctgcaaacca tggaagaaga ttttgaagaa ctgctcgaac ggtaccttca gaaagaagga
     1201 acactcgata taccgacagt gagcttttac tccaccattg caactccaat aggtactgta
     1261 caagaacggg cgcttgtaaa tgaactcaaa agaacaaaac cagcagtaaa gttgacaggg
     1321 agataattga tgctattatc catagtagaa aaaatatagt tatgatgaaa gtatttaaga
     1381 aaaaaggtgc ggcactgaaa aaggaacgtt ttcccttatc agtcaaagta gcaattaccc
     1441 gactgagaaa cactgttgca aaaaagaacg gtaagcaaaa aaaataacca ctgtacggca
     1501 catctataga ccgaacctca aaagggttcg gttttttgtt ttacccataa agcgcgtata
     1561 attgccctat gcaacctact gaaaagtttg agaccccgaa acaagaaata gactggatcg
     1621 gaaaaactgc ccaagagcga atcattcaac aggctcagga cgagcgtgct ccaaacggca
     1681 ctgacgccac caagaaaact attgaagcgt atgcaaaagc agaggcaaaa gagttcctcc
     1741 atgaaactgc catcctcccc actcatgaaa ccgagcacct ggtactcaag ctcaaacctg
     1801 aagagcacga tataaaaatt gaagaactct ttaaggtagc gctcgagaaa ggtatcaaga
     1861 acgcactcga cgttgcggca aaatttggcg acccgcatgt ggaagatgac tttcaccgct
     1921 ttttggtgca atacctgcac acaattggcg caattcccgg actcaaagaa aaatctaccc
     1981 tgcacggcga gctcgaccac tcgctcttta ctgttaccct ccctagtatg cagggagaga
     2041 gcgagaagca gaactttaaa acattcatcc aggcgatgaa gcagttctac cagagtatgc
     2101 tcgcgcttga aaaaggcgct cactacagct tggaactcgc cgtacaaaac cacgggcgcg
     2161 acatcgcgct ctatgcctcg gtgccacgcc atgggattga tatttttgaa aagaccgtcc
     2221 atgcctacta ccctggcgca agcgttgaac cggcagcaga tgactacaac atttttacgg
     2281 ttggcggagc gattgccggc gcgtatgcac aattggcaga gccggcaatc tttccgctca
     2341 acaccgatgc cgcctttgag cacgacccgc tcgccgcact tattggctcg ctctcacggc
     2401 ttgaaaccgc tggtgaaggc gcagcgatac aaatcattat tgccgcagat gaaagcaaga
     2461 tgaataaggt gtttgaaagt gttatcacaa gcgctcaaaa acagccgggc gagaaagtga
     2521 aagacttata caaggaagca actggttcaa ttcttgccgg taccggcaag gcactcttct
     2581 cgctcttttc agacggaaag aaagatgaag agaagaaaga aaaaccggta gacacgcacg
     2641 gcattgagct cgccaccgca aaaaataaaa gccagactat taacgctgca gtgcgtgtta
     2701 ttgccagcgc gcaaacacct gaccgagcag caagtattat tacaacagta aaatcatcgt
     2761 ttaatcaatt ctcgctcgtt ggttcaaata gcattgaatg gaaagacatt aaaactagcc
     2821 acctcttcgg cttctgccaa gcattttcgt tccgctctcg gaaccctgat gaagagctct
     2881 tgctctcgat cgatgaactc gcaacg
//
LOCUS       DATIBQ010000065         8382 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U16294
            k141_696798, whole genome shotgun sequence.
ACCESSION   DATIBQ010000065 DATIBQ010000000
VERSION     DATIBQ010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935789
            Sequence Read Archive: SRR25158331
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 8382)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8382)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:58:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 637
            CDSs (total)                      :: 603
            Genes (coding)                    :: 601
            CDSs (with protein)               :: 601
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8382
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_696798"
                     /isolate="SMAG_U16294"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..387
                     /locus_tag="VLB02_02075"
     CDS             <1..387
                     /locus_tag="VLB02_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HSE56851.1"
                     /translation="LAHVAIIATGSLVHHALVAARDLEKKGIKAVVVNIHTIKPLDTT
                     LIEAVAKEAKAIVTVEEHQIAGGLGGAVAEFLAQTLPTPIEFIGVHDQFGQSGTMTQL
                     IEHYGMGREAIKAAVEKVIARKNKSF"
     gene            complement(417..776)
                     /locus_tag="VLB02_02080"
     CDS             complement(417..776)
                     /locus_tag="VLB02_02080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56852.1"
                     /translation="MENKEYFKDGISNTEKEQLRNTIDTVTEDKSLSIGKKIDTLENL
                     LQLIRTREDLLVAQQNEPSVDTAHGLDLINKDIEDLAAKIGVPKEVTREELIEKEREL
                     EKDSRKARESKNAPNQN"
     gene            complement(784..1773)
                     /locus_tag="VLB02_02085"
     CDS             complement(784..1773)
                     /locus_tag="VLB02_02085"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HSE56853.1"
                     /translation="MEKQFDFMAIGDIVTDAFIRLKDAEVHCDIDEAHCKLCVRFGDK
                     IPYESVEEVKAVGNSANAAVAAARLGLSASLLAHVGNDYQGTGCLDTLKAEQVDTSLI
                     GVHDGLPTNYHYVLWYGVERTILVKHTHFPVSFPMGLQPPKMMYLSSLGDGTEAYHQE
                     IAAYIEAHPEIKLVFQPGTFQMKMGIELLARLYKHTYAFFCNVEEAQRILKTEEKDIN
                     ILTAKIHELGPRLVFIHNGIKGAYGSDGTNLYYVPVYPHDPFERTGAGDAFASTVSAA
                     LLMGKTLEEALLWGPVNSMSVVQQVGAQKGLLSREKLEAYLKIAPADYQIKKL"
     gene            complement(1823..2326)
                     /locus_tag="VLB02_02090"
     CDS             complement(1823..2326)
                     /locus_tag="VLB02_02090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56854.1"
                     /translation="MKQGIIFIVLLLSSIRETKSQIRDCLKNKEYILLWQKQVDDSVH
                     EFKVTLYLKPGYHFFSQTQDEPKAPKTVCAFEVGDSIQLINKPVEIGVRKLDAQGRHY
                     YEDSVSISQRLVVRRKDLLQLPFGDNKIDLTVTCRFRKKNEQVQQGTIHILTSPWPGT
                     QITPIEF"
     gene            complement(2453..2896)
                     /locus_tag="VLB02_02095"
     CDS             complement(2453..2896)
                     /locus_tag="VLB02_02095"
                     /inference="COORDINATES: protein motif:HMM:NF022853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD
                     domain-containing protein"
                     /protein_id="HSE56855.1"
                     /translation="MKKVLFLVLLVALCLPVATQAQIAPQTPVIKKINETTSHIEFTV
                     LIDPGWHVYAQEQPSHLPVETVFNLKAGTNIKLIGPAKEIGVIRKLDKNGKTVHVYEK
                     TVTFVQEVKMLQKMKDAKLTMSYKIGYGNTKIYKQIRSSKSTLPI"
     gene            3009..3866
                     /locus_tag="VLB02_02100"
     CDS             3009..3866
                     /locus_tag="VLB02_02100"
                     /inference="COORDINATES: protein motif:HMM:NF013296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HSE56856.1"
                     /translation="MKSLREYVQEAAGENRAIGHFNFSNLEGLWAIVRAAQAAGQPVI
                     VGTSEGERDVVGRNQAVALVRSIREELGHPIFLNADHHYSVESVKAAIDAGYDSVIFD
                     GAKLSLEENIAQSKECVAYARASGRDVVVEVELGYIGQGSNVLDGVPEGVMLDNLTKP
                     EDAKRLIAETGADLLAPAVGNIHGMFKGGVDPRLDIARIAEIREAAGVPLVLHGGSGN
                     DADFGAAIEAGMAIVHINTELRATYTRALKLFLQENPDETTPYKYGKAAGAAMQKVAE
                     EKIKTFARL"
     gene            3863..5530
                     /locus_tag="VLB02_02105"
     CDS             3863..5530
                     /locus_tag="VLB02_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HSE56857.1"
                     /translation="MNLQDELSKFITGEVQSDDVTLATYSHDASLFEVKPQVVVFPES
                     AMEVQKVVKWVAEQKALNPELSITARSAGTDMSGGPLNESIILDFTKYINRIISVDTE
                     RGIVEPGCYYRDFEKETLKLGRLMPAYTASKEICAMGGMVANNSGGEKSIKYGKVENY
                     VRWQKVIFSDGNAYEVKPLNQEELKLKMEQNDFEGYVYKKLFKLIEANYDAIQAAKPK
                     VNKNSAGYYLWNVWDRETGVFDLNKLLVGAQGTLALTTEIEFGLVPVQKHSRMVVVFM
                     NDIERLGGLVDTVMQFKPESLESYDDYSMKLAIKFAFDFFKQLGFFGALKLGFQFIPD
                     MLKVITGGVPKLIMMVEITGDDEEALYARCVEIQKAIAPFNYQSRIAGSKAEVEKYWK
                     IRRESFNLLRKHVKGKRTAPFIDDIVVTPHDLPKFFPELQKILGEYKLIYTIAGHAGN
                     GNFHIIPLMDLDNPLSADVILELSDKVYRLVAQYGGSTTAEHNDGIIRTPYLSYIFNT
                     EIINLFAEVKRIFDPQNIFNPGKKVGGSFDYIRTHLIKSDRKVAHGS"
     gene            5571..6203
                     /locus_tag="VLB02_02110"
     CDS             5571..6203
                     /locus_tag="VLB02_02110"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HSE56858.1"
                     /translation="MLTLEAKKRTTENPATIREGGFIPGIYYGAGKEAASLMVSAIPF
                     AKVFREAGETSAVTLDFGTEKVSTLIHDVQFDPVTNMPIHVDFLVVDMNKEIEVPVTL
                     EFVGVAEAEKLGLGTLVKVIREVHIRALPGDLPHALQVDVSVLKTLEDKVHVSDLVLP
                     KGVTCVTPGEEVVALVTAFVEEKEEAPVDLSAIEVEKKGKKEDESADTTA"
     gene            6252..7673
                     /locus_tag="VLB02_02115"
     CDS             6252..7673
                     /locus_tag="VLB02_02115"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HSE56859.1"
                     /translation="MTKTRIFFIARSILISACILGMVLPLPISTSTSITVEAQTAELS
                     LEERAKLEAELKVLEKEIAEKEAILKQQQKETGTIQKDVKLLTAQIETAKTKIKSKQL
                     AIQKINQEIGQKTKAINDLDSELGREHSSLKQLVKKTQEIDDKGAFYVLLSSKSISNF
                     YEDLDDFLSIQRSLQVSVQKIKHIKSQTEDQKEQLIDKQEQEADAKADLESQKKKVEL
                     SEKEKRQLLSISQTKEKEYQVVLAERQKKAQEIRNKLFSFAGGATKAIPFAEALQYAN
                     EAAEKTGVRAAFVLAILTQESNLGKNVGTCNRASDPVSKKWQNIMPGPAHKAAGKSSR
                     DDQTVFLQITKALGLDPDSTPLSCPLAGGGWGGAMGPSQFIPTTWQSVAAKVASLVGV
                     GTASPWRARDAIIASSYYLSLRGGAGGNAANERNAACRYYSGRACDGKKPANSFYGNN
                     VMALSRAIQADIDYLNQYGISRR"
     gene            7739..7993
                     /locus_tag="VLB02_02120"
     CDS             7739..7993
                     /locus_tag="VLB02_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013064062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HSE56860.1"
                     /translation="MKKDIHPKGYRPVLFHDNSSGAQFLVASTVQTTETGTFEGKEYP
                     LYRVEISSASHPFYTGTEKVIDTAGRVERFKKRAGAAAKK"
     gene            8042..>8382
                     /locus_tag="VLB02_02125"
     CDS             8042..>8382
                     /locus_tag="VLB02_02125"
                     /inference="COORDINATES: protein motif:HMM:NF015427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PCRF domain-containing protein"
                     /protein_id="HSE56861.1"
                     /translation="MIDLEAIKKNPRTQYLGHEYERLEHQAVQVRALAADAAMKELAE
                     EELQAIVVQQAALLVQIKAVEDAEKEEEEFPNEIILEVRAGAGGDEAALFAYELAQMY
                     ERYAEAQGWNWR"
BASE COUNT         2332 a         1761 c         2043 g         2246 t
ORIGIN      
        1 ttggcacatg tagcaataat tgccaccggc tcgctcgtgc accatgcgct tgtcgcggca
       61 cgcgatttag agaaaaaggg gattaaagca gtggtggtga atattcatac cataaaaccg
      121 ctcgatacaa cgcttattga agcggtggca aaagaagcaa aggcaattgt tacggttgag
      181 gaacaccaga tcgctggcgg acttggcggc gcggtagcag aatttttagc acaaaccttg
      241 ccaacaccaa ttgaatttat cggtgtccat gaccagtttg ggcagtcggg aacgatgacc
      301 caacttattg agcactacgg catgggtcgc gaggcgataa aggcggcggt ggagaaagtg
      361 attgcaagga aaaataaatc tttttagtgg ttattcttta tggttacatt aactagttag
      421 ttttgatttg gagcattttt tgattctcgt gcttttcttg aatctttttc tagctctctt
      481 tctttttcga taagctcttc cctggttacc tcttttggaa cgccaatttt ggcagctaaa
      541 tcttctatat ctttatttat caaatcaagc ccatgagctg tgtcgacact aggttcattt
      601 tgctgtgcca caagcagatc ttcccttgtt cttataagtt gtaacaggtt ctctaaagta
      661 tcaatttttt tccctatact aagactttta tcttctgtta ctgtatcgat agtatttcta
      721 agttgttctt tttcagtgtt tgatattcca tctttaaaat attccttgtt ttccatagtg
      781 taattatagc ttcttaatct ggtaatccgc tggagcaatt tttaagtacg cttcaagttt
      841 ctcgcgcgag agcagccctt tttgtgcgcc aacctgctgc accactgaca tcgagtttac
      901 tggtccccac aatagtgctt cttctagcgt tttacccata agaagtgcag ctgaaacagt
      961 agaagcaaag gcatcaccgg cgccggtgcg ttcaaagggg tcgtgcggat acaccggaac
     1021 gtagtacaga ttggtgccgt cagaaccgta tgctccttta ataccattat gaataaatac
     1081 aagccgtggg ccaagttcat gaatctttgc agtgagtatg tttatatctt tttcttcggt
     1141 tttcaaaatc cgctgtgctt cctcaacgtt acagaaaaat gcgtaggtgt gcttatagag
     1201 tcgggcgagc agttcaatgc ccatcttcat ttggaaggtg cccggctgaa acactaattt
     1261 tatttctggg tgggcttcta tgtaggcggc aatttcctga tggtacgctt cagtaccgtc
     1321 gccaagcgaa gataggtaca tcatttttgg cggctgcaaa cccattggga acgaaacagg
     1381 gaaatgggtg tgctttacca gaatggtgcg ctcaacgccg taccagagca cgtagtggta
     1441 gttggttggc agaccatcgt gtacacctat aagtgaggta tcaacctgct cagccttcaa
     1501 tgtgtctaag catcctgtac cttggtaatc gttacctaca tgtgccagaa gggaagccga
     1561 gagcccgagc cgagccgctg caacggctgc atttgccgaa tttccaaccg cttttacttc
     1621 ctcaaccgat tcataaggaa ttttgtcgcc aaaccgtacg cagagcttgc agtgtgcttc
     1681 atcaatatca cagtgcacct ctgcatcttt cagcctaata aatgcatcag tcacaatgtc
     1741 accgattgcc ataaagtcaa attgtttctc catggctaca gtataacaaa aaagccgctc
     1801 ttgagcggct ttgtataggt atttaaaatt ctataggcgt aatttgggta ccgggccatg
     1861 gacttgtcag tatatgaatg gttccctgct gtacctgttc attctttttc ctgaatctgc
     1921 aggtgacagt aagatcaatt ttgttatccc caaagggaag ctgtaacaaa tcctttcttc
     1981 tcactactag ccgctgggaa attgagacac tgtcttcgta ataatgacgc ccctgagcat
     2041 ctaattttct tacaccgatt tctacgggct tatttataag ctgaattgag tctccaactt
     2101 caaatgcaca cactgtttta ggcgctttag gttcatcttg cgtttgggaa aaaaaatgat
     2161 acccaggctt tagatataaa gtgactttaa actcatgtac tgagtcatca acctgttttt
     2221 gccacaatag tatatattct ttatttttta aacaatcacg tatttgtgat tttgtctctc
     2281 ttatgctgct gagcaacaga acaataaaaa tgataccttg tttcatgtta tctggtttta
     2341 atgatttaaa tgagcaatta aacctaaatt accttaaatt tttgttttgt agaggccatt
     2401 aaaaattgcc gatgggcggc agcttttatt ttttagtaaa cactacttac cattaaatag
     2461 gtaatgtcga tttagaagat cgaatttgtt tgtagatctt tgtattgcca tacccaattt
     2521 tgtatgacat tgtgagtttg gcatctttca ttttttggag catcttcact tcctgcacaa
     2581 aagttactgt tttttcatac acatgcaccg tttttccgtt tttatccagc tttctaataa
     2641 ctcctatctc ttttgctggt ccaattagct tgatattggt acctgcctta agattaaata
     2701 cagtttcaac gggtaagtgg cttggttgtt cttgagcata tacatgccaa ccaggatcga
     2761 ttaaaactgt aaattcaatg tgggatgttg tctcgttaat tttcttgata acgggagttt
     2821 ggggtgctat ttgtgcttgc gtagccaccg gtaagcatag tgctaccagt aagacgagaa
     2881 aaagtacttt tttcataata aacaattttt gttaaatttt cttgattatg cttgaatttt
     2941 atattttgta aatagtccct gaaaaccagt tattttttaa atggacgctc tcttgctaca
     3001 attgacgtat gaaatctttg cgtgagtatg tgcaggaggc agcgggagag aatcgggcaa
     3061 ttgggcactt taacttttca aaccttgagg ggttgtgggc aattgtgcga gcagcgcagg
     3121 cagcagggca gccggtaatt gtggggacga gcgaaggcga gcgcgatgtg gtgggtcgga
     3181 atcaggcagt agcgctcgtg cgtagcattc gcgaagagct gggccaccca atatttttaa
     3241 atgctgacca ccactactcg gtagaatcag taaaagcggc gattgatgcc gggtatgatt
     3301 cagttatttt tgatggcgca aaacttagct tggaagagaa cattgcgcaa tcaaaagagt
     3361 gcgttgcgta tgctcgcgca agcggccgcg atgtagtggt agaagttgag cttggctaca
     3421 ttggtcaggg gtcaaatgtg ctcgacggcg ttcctgaagg agtaatgctc gataacctta
     3481 caaaaccaga agatgcgaag cgactgatcg cagagacagg cgcagacttg ctcgcgccgg
     3541 cagtggggaa catccatggc atgtttaagg gtggtgttga tccgcggctc gatattgcgc
     3601 gcattgcaga aatccgcgaa gcagcagggg tgccgctcgt gctccatggc ggctcaggta
     3661 acgatgctga ctttggtgca gcaatcgagg ctggcatggc aattgtgcat attaataccg
     3721 agcttcgggc gacctatact cgggcactca agcttttttt gcaggaaaat ccagacgaaa
     3781 caacgccgta caagtatggc aaagcggcag gggctgccat gcaaaaagta gctgaggaaa
     3841 aaattaaaac ttttgcccgg ctatgaacct ccaagacgag ctctcaaaat ttataacggg
     3901 cgaagtgcag agcgatgatg tgacacttgc cacctattcg cacgatgcca gtctttttga
     3961 ggtgaagccg caggtggtgg tgtttcctga aagcgcgatg gaagtacaaa aagtggtgaa
     4021 gtgggtagct gagcaaaaag cgttaaaccc tgaactttct attaccgctc gctctgcggg
     4081 tactgatatg agcggcggac cattaaacga atcaatcatt ttagatttca caaaatacat
     4141 aaaccggatt atttcggttg atactgagcg tggaattgtt gagccaggat gctactaccg
     4201 cgattttgaa aaagaaacgc tgaagctcgg gcgattaatg ccggcgtaca ctgcctcaaa
     4261 agaaatttgt gcgatgggcg gcatggtagc aaataattca ggcggggaga aatcaatcaa
     4321 gtatggcaaa gttgaaaact acgttcgctg gcagaaggta atattttcag acgggaatgc
     4381 gtatgaggta aagccactca atcaagaaga attgaagctt aagatggagc agaacgattt
     4441 tgaagggtac gtgtacaaaa aactcttcaa acttattgaa gcaaactacg atgcgatcca
     4501 ggcagccaaa ccaaaggtaa ataaaaactc tgccggctac tatttgtgga acgtgtggga
     4561 tcgggagaca ggggtgtttg atttaaataa actactcgtt ggggcacagg gaacacttgc
     4621 actgaccact gaaatagagt ttgggttggt accggtgcaa aagcattcac gaatggtggt
     4681 ggtattcatg aacgatatcg agcgcttggg cgggctggtt gatactgtaa tgcagtttaa
     4741 gcccgagagt ttggaatcgt acgatgacta cagcatgaag ctcgcgataa agtttgcctt
     4801 cgacttcttc aagcagctcg gcttttttgg cgcattaaaa cttgggttcc agttcatccc
     4861 cgatatgctg aaggttataa cgggtggtgt gccaaaactc attatgatgg ttgagattac
     4921 tggagatgat gaggaggccc tatatgcgcg ctgtgttgag attcaaaaag cgattgcgcc
     4981 ctttaactac caatcacgga ttgccggttc aaaagctgag gttgaaaagt actggaagat
     5041 ccggcgcgag agctttaacc tgctgcgcaa gcacgtaaag gggaagcgca cggcaccgtt
     5101 tattgatgat attgtggtta ccccgcatga cctgccaaaa tttttccccg agctacaaaa
     5161 aatccttggc gagtacaagc ttatatacac gattgctggt cacgcaggga atggtaactt
     5221 tcatatcatt ccgctcatgg atctcgacaa tccgctctcg gctgatgtta tcctagaact
     5281 ctctgataag gtgtaccgat tggtagcaca gtatggtggc tcgaccactg ccgagcacaa
     5341 cgacggcatt atccgcactc catacttgtc atacattttt aataccgaaa tcattaatct
     5401 atttgccgaa gtaaaacgca tttttgatcc gcagaacatt tttaacccag gcaagaaggt
     5461 aggcggctca ttcgactaca tccgcaccca cctcataaaa tcagacagaa aggtcgcaca
     5521 tggttcgtag ggggttgcat tttgatactt gttttgtata atgaccgcct atgctaacac
     5581 ttgaagcgaa gaagcggaca actgaaaacc ctgcaacaat ccgcgaggga ggctttatcc
     5641 cgggtattta ctatggcgcc ggaaaggaag cggcttcgct catggtttct gcaattccgt
     5701 ttgcaaaggt attccgcgaa gcgggggaaa cttcggcggt aacactcgat tttggtactg
     5761 aaaaggtaag caccctcatc cacgatgtcc aatttgaccc agtaacgaac atgccaatcc
     5821 acgttgattt cttggtggtg gatatgaata aagaaattga ggtaccagta acactcgagt
     5881 tcgttggtgt tgctgaagct gaaaaactcg gtttgggaac cttggtgaag gtgattcgtg
     5941 aagtacacat tcgtgcactc ccgggcgact tgccacatgc gctccaggtt gatgtttcag
     6001 tacttaaaac acttgaagat aaagttcatg taagtgattt ggtgctgcca aaaggggtaa
     6061 cctgcgttac tcctggtgaa gaagtagtag cactcgtaac tgcattcgtt gaagaaaaag
     6121 aagaagcacc agttgatctc tcggcaatcg aggtagaaaa gaaaggtaag aaagaagacg
     6181 aatcagccga tactactgcc taaccgtcaa cgtaagaagt ttgctatact aaagtcctat
     6241 ctatgtcacc catgactaag accaggattt tttttattgc ccgctctatt ttaataagcg
     6301 cttgcatact cggcatggta ttgccgctgc caattagcac cagtacttca attactgttg
     6361 aggcgcaaac ggcagagctt tcgctcgaag agcgggcaaa acttgaggct gaactgaagg
     6421 tgctcgagaa agaaattgct gaaaaagagg caattttgaa gcagcagcag aaagaaactg
     6481 gcacgattca aaaagatgta aagttgttga ctgctcagat tgagaccgca aaaacaaaaa
     6541 tcaagtcaaa gcagctggca atacaaaaaa ttaatcaaga aatcgggcag aaaactaagg
     6601 caattaacga tcttgattcc gagcttggtc gtgagcacag cagcttaaaa cagttggtaa
     6661 aaaaaacgca ggagattgat gataaaggcg ctttttacgt actcctctcg agtaaatcaa
     6721 tttcaaattt ctatgaagat ttggatgact ttctctctat tcagcgctcg cttcaggtgt
     6781 cggtgcaaaa aataaagcat attaaatcgc agaccgaaga tcaaaaagag cagttgatcg
     6841 acaagcagga gcaagaggca gatgcaaaag ctgacttgga atcgcagaag aaaaaagtag
     6901 agctttctga aaaagaaaaa aggcagctct tgtcgattag tcagacaaaa gagaaagagt
     6961 accaggtggt gcttgctgag cggcagaaga aggcgcagga aattcgaaac aaactcttca
     7021 gtttcgccgg cggcgcgaca aaagcaattc cgtttgctga agcgctccag tatgcgaatg
     7081 aagcagcaga gaaaacggga gtccgagctg cgtttgtgct tgctattctt actcaggagt
     7141 caaacctggg gaaaaacgtc ggaacctgta accgtgcgag tgatccggta tcaaaaaagt
     7201 ggcagaatat tatgcctggt ccggcacata aagcagccgg aaaatcgagt cgtgatgatc
     7261 aaactgtatt tttgcaaata actaaagcat tagggctcga ccctgactca acaccactct
     7321 cgtgcccgct tgctggcggt ggctggggtg gggcgatggg accttcacag ttcattccaa
     7381 ccacctggca gtcggttgcg gcaaaagttg cttccctggt aggggtgggc acagcaagcc
     7441 catggcgagc tcgagatgca ataattgctt cttcgtacta cttgtcgctt cgtggcggag
     7501 caggggggaa tgccgcaaac gaacgtaatg ctgcctgtcg ctattattcg gggcgcgcct
     7561 gcgacggaaa gaagccggca aacagctttt atggcaataa cgtgatggcg ctctcacgag
     7621 ccattcaggc tgatattgat tatctgaacc agtacggaat ttcacggcgc taggacccgt
     7681 cctgagcgga gccgaaggga agttgatttt ttcgtccctc tatggtaaaa atacccctat
     7741 gaaaaaggac atccacccaa aaggctatcg accagtgctc ttccatgata attcgtcagg
     7801 agcacagttt ttggttgcct caaccgtgca aacgaccgaa actggtacct ttgaaggcaa
     7861 agagtaccca ctctaccgag ttgaaatctc aagtgcttcg cacccgttct ataccggtac
     7921 tgaaaaggtt attgacaccg caggacgtgt tgaacgcttc aagaagcgcg ctggtgcagc
     7981 ggcaaaaaaa taactctcct gtatactgaa gaagccgtgc aagcggtttt ctttcatttc
     8041 tatgatcgat cttgaagcaa taaaaaagaa tccgcgcacc cagtacttgg ggcacgagta
     8101 cgaacgcttg gagcaccagg cggtgcaagt tcgagcactt gcagccgatg ccgcaatgaa
     8161 ggagctcgct gaagaagagc tccaggcaat tgtggtgcag caggctgcac ttctggtaca
     8221 gattaaagca gtagaggatg ctgaaaaaga agaggaagaa tttccaaacg aaattatttt
     8281 agaggtacgc gccggagcag gcggcgatga agcagcactc tttgcgtatg aacttgcgca
     8341 gatgtatgag cgctacgctg aagcacaggg ctggaattgg cg
//
LOCUS       DATIBQ010000066         4807 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U16294
            k141_1680269, whole genome shotgun sequence.
ACCESSION   DATIBQ010000066 DATIBQ010000000
VERSION     DATIBQ010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935789
            Sequence Read Archive: SRR25158331
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 4807)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4807)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:58:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 637
            CDSs (total)                      :: 603
            Genes (coding)                    :: 601
            CDSs (with protein)               :: 601
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4807
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1680269"
                     /isolate="SMAG_U16294"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..259
                     /locus_tag="VLB02_02130"
     CDS             <1..259
                     /locus_tag="VLB02_02130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56862.1"
                     /translation="LKKLYDTENSFTMNANIPAAPHGYLKLFLMIAPDCFVDGFKTFK
                     AEKKKIVLQVLKKMFKNTKHLNALAQKPVIVDEYGNIFEPK"
     gene            350..432
                     /locus_tag="VLB02_02135"
     tRNA            350..432
                     /locus_tag="VLB02_02135"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:383..385,aa:Leu,seq:caa)
     gene            437..518
                     /locus_tag="VLB02_02140"
     tRNA            437..518
                     /locus_tag="VLB02_02140"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:471..473,aa:Leu,seq:cag)
     gene            complement(1360..1431)
                     /locus_tag="VLB02_02145"
     tRNA            complement(1360..1431)
                     /locus_tag="VLB02_02145"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1396..1398),aa:Trp,seq:cca)
     gene            complement(1479..1637)
                     /gene="rpmG"
                     /locus_tag="VLB02_02150"
     CDS             complement(1479..1637)
                     /gene="rpmG"
                     /locus_tag="VLB02_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007438733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HSE56863.1"
                     /translation="MSQQNLVKLVNKETGEVYWTRKNRKLLANVKLEFKKFSKKLRKV
                     VVFKESKK"
     gene            1734..2192
                     /locus_tag="VLB02_02155"
     CDS             1734..2192
                     /locus_tag="VLB02_02155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56864.1"
                     /translation="MEQTTTATQSKPSYIFPKRRQKFTVKGLSPYAMQHSDATQKIGM
                     LDKNKVCLSPCGSGTIWENCKNSETGKTCLDIEIAIQLFEDPIFREKITQELPPKKVV
                     IILCGGTTFILGNDTYIPYFEYTPKSDTLAIGLIPTEYAGKVENAFLLVF"
     gene            complement(2243..2623)
                     /locus_tag="VLB02_02160"
     CDS             complement(2243..2623)
                     /locus_tag="VLB02_02160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56865.1"
                     /translation="MEQNRGFTFAIGLVALVLGVVVGVALCSGSRVRVATDVVGRDFR
                     AGQPYHYADSYYRTDRLRASVYDSVSRRENVYYEYSNIPETITFHSTDCTNPATIDRY
                     SRQLNNQAFQDFANFCESVGQPIG"
     gene            complement(2671..4179)
                     /locus_tag="VLB02_02165"
     CDS             complement(2671..4179)
                     /locus_tag="VLB02_02165"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HSE56866.1"
                     /translation="MSKFIALKNISHSFLERPLLEGLSFKLTDQDRTCILGENGTGKS
                     TLLKIIAGTLEPTSGHIEKNTHIRCYYVAQEFTGEDLLLTVEGFIEKFGSVTLLKKIF
                     SISTTLGFNLEKALNSVCKSLSGGQQKILALSIGLASSPDFLLLDEPENHLDIISRLE
                     LIKLLQNYRGGVVFVSHDRLMIDAIATKVAEVTGKKLYLSEGSYQEYIDARLSRIAGL
                     QRSFDTETKRIRQLENMLPILQQKAFRGKEVSSYLKRKAELETLREKHADEPRADDRK
                     TKIRLQQSTSDLHRGKLLCRIENARFTYEGKKGDIFREVQLDIRSGKHIVLLGRNGSG
                     KSTFLKSLTNNLALTEGAITWAEHVTTSYFDQHAEFDPQKTALESVIDLLKCDDNKAR
                     SVLGAMKFTKEKMNTVTGQLSGGERMRLRFALVFGVNPDFIILDEPTNHLDEVTWQIL
                     LDACNSAKNTILLVTHDYEFIEELESKVFWLIKNQTIIERHKDLEEIIEELR"
     gene            4302..4520
                     /locus_tag="VLB02_02170"
     CDS             4302..4520
                     /locus_tag="VLB02_02170"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HSE56867.1"
                     /translation="MENELFNQPLTERDKKIMNLICHEKTVREIGEMVNLSPRTVEGE
                     IPKIAEKIGVQGKVGIAVYAVKNGIYIE"
BASE COUNT         1459 a         1066 c          972 g         1310 t
ORIGIN      
        1 attaaaaaag ttgtacgata cagaaaactc atttaccatg aatgcaaaca tccccgctgc
       61 tccacacggc tacttaaagt tgtttctcat gatcgctcct gattgttttg ttgatggctt
      121 caaaactttt aaagccgaaa agaaaaagat tgtactacaa gtgctaaaaa agatgtttaa
      181 aaacacaaaa cacttaaatg cccttgctca aaaaccagtg atagtagacg aatatggaaa
      241 catatttgag ccaaaataaa ttaaacggcc agactgtatt aaaaaacaat ctggcttttt
      301 gtttattgca aaaatctatt gttggtgtaa gaatgaattc gcattcaatg ccggagtggc
      361 ggaatggtag acgcaatcga ctcaaaatcg atcgggggta accccgtgga ggttcaagtc
      421 ctctctccgg cacccagccg cggtggcgaa attggtagac gcgcacgttt caggtacgtg
      481 tagagcaatc tgtggaggtt caagtcctct ccgcggcaca acaagaataa attttctgta
      541 tgcaacaaca aaaagtaatc gttgtctgcg gtccaaccgc aaccggtaaa tccgactatg
      601 ccgtagcctt ggcaaagaaa gttggcggcg aggtaatttc agctgattcg cgccaggtat
      661 acaaagggct tgatataggt tcagggaaaa ttacaaaaaa agaaatgggt ggtgtccccc
      721 accatctcct tgatgtcgca aacccaaagc gagtgttttc ggttgcacag taccaaaaac
      781 ttggaacaaa agcgatcagc gccattttaa aacgaggcaa ggttccgatt atttgcggcg
      841 gtaccggatt ctatattgat gcactcatct acaaaactaa cttccctgct gttaagccaa
      901 acgcggcact tcgtaaacag cttgaaaaaa aatcagctga agcactggcg caagaactag
      961 agaaacttga tcctgataga tttgaaacga ttgacgtaaa aaataaagtt cggcttattc
     1021 gcgcaattga aattgcaaca atacttggaa gcgttcctac actgcaaaga aaaactcagt
     1081 acgaagttga gtggatatac ttagattttc ctgatgaaat acttaaagag cggattcata
     1141 ctcgcctatt aaagcgcatg aaaacgggca tggttgccga agtaaaacga ctacatgccg
     1201 aaggagtttc atggaaacgg cttgagtcgc tcgggcttga ataccggtac cttgccctct
     1261 atcttcaaaa aaggctcaca aaagaagaaa tgcttgagca acttgaatct gctatctggc
     1321 actacgccaa acaccagcga acgtggttta agaagtatgc gggcccacca ggactcgaac
     1381 ctggaacttc ggttttggag accgacgttt taccattgaa actatagacc ctagaaacgc
     1441 cttacactat aacaaaagag ccccggaagg gctacttttt atttcttaga ttctttaaag
     1501 acaaccacct tgcgaagctt cttgctgaac ttcttaaact cgagctttac attcgcgagg
     1561 agctttcggt tcttgcgggt ccagtacacc tcgcccgttt ctttattcac taactttacc
     1621 agattttgct gtgacatagt gaaaagaaga taccacagaa ggcttttttt tacaatagca
     1681 cccaattgac ttttttatga tttagtgtta gtataggaaa aaacttcatt accatggaac
     1741 aaacaaccac cgcgactcaa agtaaaccaa gttacatttt tcctaaaaga aggcagaaat
     1801 ttacagtaaa aggattgagt ccttatgcca tgcaacatag cgatgccaca caaaagattg
     1861 gcatgctcga taaaaacaaa gtgtgtcttt ccccatgcgg cagcggtact atttgggaga
     1921 attgtaaaaa ttctgaaacc ggaaaaacat gtttggatat agaaatagca atacagctgt
     1981 ttgaagatcc aatattcaga gaaaaaatca cacaagaatt gccacctaaa aaagtagtta
     2041 tcattctgtg tggcgggaca acatttatcc tgggaaatga cacatacatt ccctattttg
     2101 aatacacacc aaaaagcgat actttagcca ttggtcttat accaacagaa tacgcaggga
     2161 aagtagaaaa tgcattttta ttggttttct aaaaaaacaa ggaaaccaaa atcccccgaa
     2221 caacatattc gggggatttt atttacccaa tcggttgtcc gacactttcg cagaagtttg
     2281 caaagtcttg aaatgcctga ttatttaact gccgtgaata gcggtcgatt gttgctggat
     2341 tggtgcagtc agttgagtga aaagtaattg tttcaggaat gtttgagtac tcgtaataca
     2401 cgttctcgcg gcggctgact gaatcgtaga ccgatgcacg gaggcgatcg gtacggtagt
     2461 agctatccgc atagtggtac ggctgccctg ctctaaaatc tcggccaaca acatcggttg
     2521 ctacccgaac gcgactgccg ctacagagtg ccacaccaac caccacccca aggacaagtg
     2581 ctaccaagcc tattgcgaat gtaaatcctc tgttctgttc catatactag tgatactacc
     2641 cttcttccct aaaatgtcaa tgtgccacac ttaccgcaat tcttcaataa tttcttccaa
     2701 atctttatgc cgttctataa tggtctgatt ttttatcagc caaaatactt ttgattccaa
     2761 ctcttcaata aattcataat cgtgggtaac gagcagaatg gtattttttg cactgttgca
     2821 ggcatcgagg agaatttgcc aggttacttc gtcaaggtgg ttagtaggct cgtccaaaat
     2881 aataaaatcg gggttaaccc caaacactag cgcaaagcgg agccgcatcc gctccccacc
     2941 cgagagctgc ccggtcacgg tattcatttt ttcttttgta aacttcattg ccccgagcac
     3001 cgaccgagcc ttgttatcat cacacttcaa gagatcaatc acactctcaa gggcagtttt
     3061 ttgtgggtcg aactcggcat gctggtcaaa gtagctcgtg gtaacatgtt ctgcccaagt
     3121 gatagcccct tctgtgagcg ctaggttatt agtgagcgac ttcaagaagg ttgatttgcc
     3181 cgatccattt ctccccaata acacaatgtg cttgccagaa cgaatatcta actgcacctc
     3241 tctaaagata tctccctttt tcccttcata ggtaaaccgg gcgttttcaa tccggcacaa
     3301 gagcttgcca cgatgcagat cgctggtact ttgttgcagc cgaatttttg ttttacgatc
     3361 atcagcccga ggctcatctg catgcttttc tcttaatgtt tcaagctctg cctttcgctt
     3421 cagatatgaa gacacttctt tcccccgaaa cgccttctgc tgcagaatcg gcagcatgtt
     3481 ttctaattgt ctaatacgct ttgtttcggt atcaaagctg cgctggagcc ctgcaatgcg
     3541 cgagagccgg gcatcgatgt actcctggta actcccctca gaaagataga gtttcttacc
     3601 ggtaacctcg gcaaccttgg tcgcaatcgc atcaatcatc agccggtcgt gcgagacgaa
     3661 cactaccccg ccccgataat tttggagcag ctttatgagc tccaagcggc tgatgatgtc
     3721 gaggtgattt tctggctcat cgagcagtaa aaaatcgggg ctgctggcaa gcccgatact
     3781 gagtgctaaa attttctgtt gcccacccga gagtgatttg caaacactat tcaatgcttt
     3841 ttctagatta aacccgagcg tagtactaat ggagaatatt tttttgagaa gcgtaacact
     3901 tccaaatttt tctataaaac cttcaacagt gagcagcaga tcttcaccag taaattcctg
     3961 agcaacgtag taacaacgaa tatgggtgtt cttttcaata tgccccgagg ttggctccaa
     4021 tgttccggca ataattttta agagggttga tttgccggta ccgttctcgc ctaaaataca
     4081 ggttcggtct tggtcggtta gcttaaaaga aagcccctct aagagtggcc gttctaaaaa
     4141 tgagtggctg atatttttga gggcaataaa tttagacatg caactttgtc agagtgttat
     4201 agcatcttta ccagtaaaca tccaacaaaa atttgacagg gcactatctg ccctatataa
     4261 atgcatgaaa tctaacctct ttaaatttaa ttagtacaga aatggaaaat gaacttttta
     4321 atcaacctct taccgaacgt gacaaaaaaa taatgaacct tatctgtcac gaaaaaacag
     4381 tcagagaaat tggggaaatg gtcaatctaa gccctcgcac cgtggaagga gaaattccca
     4441 aaatagctga aaagataggg gtgcaaggaa aagtcggtat cgccgtctat gccgtgaaaa
     4501 acggcatata tattgaatga aaagtaaaca aaagccgcgt atacatacgc ggctttttga
     4561 ttatgtgtta taccggagga agtaaaaact aaattaatga aaccttctat ccttccgtac
     4621 atacccaatc tgttaaaaat taaatgttct taccctacat cctcacatac tatgattaat
     4681 tcttcccctc tcttcgatat tgttctttta ttttccagta cacgtcttca gtaccagtaa
     4741 catcatcaaa aaaacagtag gttattgaga cctactgtta gattaattac ttaattttgc
     4801 tgagctt
//
LOCUS       DATIBQ010000067         8265 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U16294
            k141_2206228, whole genome shotgun sequence.
ACCESSION   DATIBQ010000067 DATIBQ010000000
VERSION     DATIBQ010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935789
            Sequence Read Archive: SRR25158331
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 8265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8265)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:58:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 637
            CDSs (total)                      :: 603
            Genes (coding)                    :: 601
            CDSs (with protein)               :: 601
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8265
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2206228"
                     /isolate="SMAG_U16294"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..879)
                     /gene="tyrS"
                     /locus_tag="VLB02_02175"
     CDS             complement(<1..879)
                     /gene="tyrS"
                     /locus_tag="VLB02_02175"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HSE56868.1"
                     /translation="MNEVVVLDSGYGKKVDAFLEKGVSEIFPNRDSVKNLLLKTEKPL
                     TLYAGFDPTAPTLHLGHTIQLRKLKQWQDMGHKVVFLVGDFTALIGDPTDKLAARTQL
                     TEREVKQNLRTYKAQAGKIIRFSGKNKAVIRFNAQWLKKMKLKDIISLSSLVTVDQMQ
                     KRDMFDRRTQEGKPIYMHEFLYPLMQGYDSVALKVDGEVGGTDQTFNMLMGRTLEQKL
                     LQKEKFVITMKLLEDGSGKKMGKSEGNMVALDEDPSQMYGKVMSWPDSVVPSAFELLT
                     DLDTEAVTTLFTATPHP"
     gene            936..3092
                     /locus_tag="VLB02_02180"
     CDS             936..3092
                     /locus_tag="VLB02_02180"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HSE56869.1"
                     /translation="MRKLHIWKVFFILVSCAFLALGGTLLWATTIELPDLSTFQARKI
                     SNSSKIVDRTGTVVLYDIHQSVRRTEIPLTGMGENIQHATIAIEDANFYSHNGIRIQS
                     IIRAVLANLFRGGAYSQGGSTITQQIIKNTLLTPEKTVTRKIKEWVLAIKLERLLPKD
                     RILEIYLNDNPYGGTIYGVEEASRAYFNKASKDLSFAEAAYLAAIPRAPSYYSPFGKH
                     QDKLEERKNFILSRMKSQNFITEEQYKTAKAEKVSFDNNASNGIKAPHFVFYIQEMLE
                     EQYGEDAMASGGYTIITTLDYELQKKAEEIVKKNALQNEKSWNASNAALVATNPQTGE
                     ILAMVGSRDYFDKGIDGAYNVATAKRQPGSSFKPFVYAAAFDKGYTPETVLFDVPTEF
                     STTCDASAADTKEGCYHPGNFDDKFRGPINLRNALAQSINVPSVKTLYLVGLDSALDT
                     ARRMGINSLRDAKYYGLSLVLGGGEVSLLDMTTAYSTFAAGGVRHDPVGILQIKDKSG
                     TVIESYTPGDGEQVLSKNTALTITDVLSDNVARAPTFGSNSALHIDGVQVAAKTGTTN
                     DNRDAWVLGYSSNLTVGVWSGNNDNKPMRKGGVALSGPIWNQFMQEALPLFPNTDFEK
                     PERNPNYDSLKPVLRGEWRALDTAIIDSRTGTVATEDTPEEFKETQTAPVVQSILYWV
                     DRKDPLGPKPQNPYSDPEFRLWNPPVQAWWTAHGVQ"
     gene            complement(3097..3360)
                     /locus_tag="VLB02_02185"
     CDS             complement(3097..3360)
                     /locus_tag="VLB02_02185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56870.1"
                     /translation="MNAFQNILKTIQERLKVSEHDSEKIVSLLIEKLGVPLQKNQIMV
                     KGNILFLEVSPLLRTEVQLKKKLLLSFLKEHFLTFSDIQIKTK"
     gene            complement(3368..4468)
                     /gene="dnaN"
                     /locus_tag="VLB02_02190"
     CDS             complement(3368..4468)
                     /gene="dnaN"
                     /locus_tag="VLB02_02190"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HSE56871.1"
                     /translation="MKVVCTGEKLKNAIILCERITGKNLTLPVLGALLLIASGKTLKI
                     RATNLSLGIEFEIPAKIETEGVVLVKGEVIAGIMGTLADSDEITLTLENENLVLKTKK
                     SKLLVKCLPTEDFPTLPAVQEGIVFSIDPKVLVQGIKAVSFAAAVSDIKPEISAIFIY
                     TDAEHIVFVATDSFRLAEKKLKLKQVPEITQLLIPFKNIAEITRVLEGVNGEVRIEFT
                     KNLISFSGGGIFLTSRLVDGIFPDYRQIIPKEWKTQCVLLKQDLSNTLRLSTIFADKF
                     QQTMFSLNPAEKTVSVTSQNADVGENTSSVDAVLKGEPIEVTINLRYFLDAFQALSGD
                     SIAIELTQPNRALVVKSVSDHNFLYLLMPLNR"
     gene            complement(4676..6016)
                     /gene="dnaA"
                     /locus_tag="VLB02_02195"
     CDS             complement(4676..6016)
                     /gene="dnaA"
                     /locus_tag="VLB02_02195"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HSE56872.1"
                     /translation="MDTELYQLWQTCLREIESQVTRANFSTWFKNTTILREEEGVLVI
                     GVPNEFVKEWLFNKYHKLILKTIVDQAQHVRAIEFTISKLESRSEQKPVIEGKGALPF
                     QDLYINKEDNLNPKYTFDRFIVGSFNELAYAAAQAVISRPSQAYNPLFIYGSSGLGKT
                     HLIQAIGNEIKAKFPEKKVLYTTLEKFANDYISAVQNNRATSFREKYRKYDFLIMDDI
                     QFLSTKEKTQEELFHLFNTLYDQNKNIVFSSDKHPNHILGIEDRLKTRFNAGMTIDVQ
                     EPDFESRLAILREKTSGMNIQLNEDILAYLATSITSSIRELEGVLNVISCHVQLKNRE
                     ISLDEVKQLIKYSVKPKKNVLVQDIVRIISNYYNVDEKIIYEKTRRKEIVRARQMIMF
                     ILREDFNASYPTIGAKLGGKDHTTVIHSYEKIKRDLLTNPLLMRELEDIRVLFK"
     gene            6165..6299
                     /gene="rpmH"
                     /locus_tag="VLB02_02200"
     CDS             6165..6299
                     /gene="rpmH"
                     /locus_tag="VLB02_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004102791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HSE56873.1"
                     /translation="MSFTYKPKKKKRAKAHGFLSRSKSKGGKKVLKARRRKGRATIAV
                     "
     gene            6305..6640
                     /locus_tag="VLB02_02205"
     CDS             6305..6640
                     /locus_tag="VLB02_02205"
                     /inference="COORDINATES: protein motif:HMM:NF013022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HSE56874.1"
                     /translation="MLPKKNKLPRAAFSELLAQNPISVFNRVGTLKYIPHSGSFSVVV
                     SAKHCKRAVARNKVKRRVYSLIQVFKKEYTTPLSGILYLSKHAYMMPFSELKILVQEL
                     LRRAYANSK"
     gene            6624..6869
                     /gene="yidD"
                     /locus_tag="VLB02_02210"
     CDS             6624..6869
                     /gene="yidD"
                     /locus_tag="VLB02_02210"
                     /inference="COORDINATES: protein motif:HMM:NF013932.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HSE56875.1"
                     /translation="MRIVNSAAIFLIHIYQAIFSPATGILRFIPFYPKPSCIFYPTCS
                     EYAVLAFRKYGFLKAFFISTKRIMRCHPGNEPKIDYP"
     gene            6892..7614
                     /locus_tag="VLB02_02215"
     CDS             6892..7614
                     /locus_tag="VLB02_02215"
                     /inference="COORDINATES: protein motif:HMM:TIGR03592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HSE56876.1"
                     /translation="MKSLWNTLIYHPLLNILVALAAVMPGKSLGLAVIVLTILIRLLL
                     FPISKRAIRSQYALRALEPKVQAIKAKKLDKQEESRALFQLYKEEKVNPFSGCLLLLI
                     QLPILIALYYVFINGINRPELLYSFISPEGIQPTLFGLSITAPSIVLAIFAALTQAVQ
                     AFLTPKPPKPAESDKINFQSQLASSMSIQTRYVLPIIIFFIARNLAAAVSLYWVVSNL
                     FSIGQEIYFRRLFKHKALIKQS"
     gene            complement(7598..>8265)
                     /locus_tag="VLB02_02220"
     CDS             complement(7598..>8265)
                     /locus_tag="VLB02_02220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56877.1"
                     /translation="FLRDNISSMAVFADGKQLATLTDEQGESVVRISNPDGSKEQAFF
                     KYPFKDWLLLASRLELYLQTKADSSFAGSLFQLSKNDYKKIMISIPGLTAAVNPAGQY
                     IVSATATGSSFTSSLFNTKTGETKPLTVPVLPEKCVWTADQTFICATPKNIPTAGYPE
                     SWYRGRVHFSDNLVVINPSSGQTQLKAVSGVFDITAPQYSSVTKTLYFIDKQSGILFK
                     TAL"
BASE COUNT         2213 a         1882 c         1765 g         2405 t
ORIGIN      
        1 tgggtgcgga gtagcagtaa agagtgttgt cactgcctca gtatcaagat cagtaagcag
       61 ttcgaacgcg ctcggcacta cactatcagg ccagctcatc acttttccat acatctggct
      121 tgggtcttca tcgagcgcaa ccatgttccc ctcgctcttg cccatctttt tgcctgagcc
      181 gtcttctaag agcttcatcg ttataacaaa cttctctttc tggagcagtt tttgctcgag
      241 cgtgcgcccc atgagcatgt tgaaggtttg gtcggttccg cctacttctc cgtcaacctt
      301 gagcgcgaca ctgtcgtacc cctgcatcag cgggtacaga aattcgtgca tataaattgg
      361 ctttccttct tgggtgcggc gatcaaacat atcgcgcttt tgcatttggt caacagtaac
      421 caaagaagag agcgagatga tatctttgag cttcattttc ttcagccact gtgcattaaa
      481 ccgaataact gctttattct ttcctgaaaa acggataatt tttcctgctt gtgccttgta
      541 tgtccgtaag ttttgtttta cttcgcgctc ggtaagctgg gtgcgcgctg caagtttatc
      601 ggttgggtcg ccgatgagcg cggtaaaatc cccaaccaaa aatacgacct tatgccccat
      661 atcctgccac tgtttaagct tgcgcagctg tattgtgtgc cccaggtgca gtgttggtgc
      721 ggttggatca aaccctgcgt agagcgtgag cggtttttca gttttaagta aaagattttt
      781 aacagaatca cgatttggaa atatctcaga cactcccttc tctaaaaaag catcaacttt
      841 tttgccatag ccgctatcga gcaccactac ttcattcata ccgccgattc tatcatcttt
      901 cttccccttt tacagctttt tgctatgatg cctttatgcg aaaactacat atctggaaag
      961 ttttttttat tcttgtttcg tgcgcgtttt tagcgctcgg cggcacactc ttgtgggcaa
     1021 ctactatcga gctgcccgac ctttcaacat ttcaagcgcg caaaatctca aactcgtcta
     1081 aaatcgttga ccgcaccggc accgtagtgc tctacgacat ccaccagtca gtccgccgga
     1141 ctgaaattcc gctcaccggg atgggcgaga atatccagca cgcaaccatt gctattgaag
     1201 acgcaaattt ctacagccac aacggtatcc gcattcaatc aattatccgt gcagtacttg
     1261 ccaacctgtt tagaggcggc gcgtattcgc agggcggctc aaccattacc cagcaaatta
     1321 tcaaaaatac cctccttacc cccgaaaaaa cagtaacccg aaaaattaaa gagtgggtgc
     1381 tggcaataaa actcgagcgg ctcctgccga aagataggat tctggaaatt tacctcaacg
     1441 acaatcccta cggcggcaca atttatggag ttgaagaagc gagccgtgca tacttcaata
     1501 aagcctcaaa agatctctct tttgctgagg cagcctacct tgctgctatt ccccgagcac
     1561 cgagctacta ctcaccgttc ggcaaacacc aagacaagct tgaagagcgg aaaaatttta
     1621 ttttgagtcg aatgaaaagc cagaacttca ttactgaaga acagtataaa actgctaaag
     1681 cagaaaaagt atcattcgat aacaacgcat caaatggtat taaggcacca cactttgttt
     1741 tctatatcca agagatgctt gaagagcagt acggggaaga tgcgatggct tcgggcgggt
     1801 acactattat tactacgctt gactacgagc tccagaaaaa agcagaagaa attgtaaaaa
     1861 agaatgcgct ccaaaacgag aagagttgga atgcctcaaa tgcggcgctc gttgcaacca
     1921 acccgcagac gggtgagatt ttagcgatgg tcggctcacg agactacttt gataaaggga
     1981 ttgacggcgc gtacaacgtt gccaccgcaa agcgccagcc aggatcgtca tttaaaccat
     2041 tcgtctacgc cgccgctttt gataaaggct acacccctga aacggtactt tttgatgtgc
     2101 cgactgaatt ttcaactact tgtgatgcct cagctgctga cacaaaagaa ggctgctacc
     2161 atccgggaaa ttttgatgat aaattccgcg gaccaatcaa cctgcgcaac gcactcgcgc
     2221 aatcaattaa cgttccttca gtaaaaacgc tttacttagt tgggctcgac agcgcactcg
     2281 atactgctcg gcggatggga atcaattcac tccgcgacgc aaaatactat ggactctcac
     2341 tggtgctcgg tgggggggaa gtatcactct tagatatgac aactgcatac agcacgtttg
     2401 ctgcaggcgg tgttcgccat gacccggttg gcattttgca aattaaagat aaatcaggaa
     2461 ctgttattga gtcatatact ccgggcgacg gcgaacaggt gctttcaaaa aatactgcgc
     2521 tcactattac cgacgtactg agcgacaacg tcgcccgagc accaaccttt ggctcaaact
     2581 cagcactcca tattgatggc gtgcaggttg cagcaaaaac tggaaccacc aacgataacc
     2641 gcgacgcctg ggtactcggc tactcatcga atcttacggt gggggtatgg tcgggtaata
     2701 acgacaacaa acctatgcgc aagggtggtg tagccctctc aggacctatc tggaaccagt
     2761 ttatgcaaga ggcgctcccc ctcttcccaa acaccgattt tgaaaaacca gagcgaaatc
     2821 cgaattatga ttcactaaaa ccagtgctcc gcggcgagtg gcgtgcgctc gacacagcaa
     2881 tcatcgactc gcgcacgggt acagttgcaa ctgaagacac tcctgaagaa tttaaagaaa
     2941 ctcaaacagc tccagtagtg caatcaatcc tctactgggt agatagaaaa gacccgctcg
     3001 gaccaaaacc gcaaaacccg tacagcgacc ctgaatttag actctggaat ccgcccgtac
     3061 aggcatggtg gacagcacat ggcgtgcagt aaaaaactat tttgttttta tctgaatatc
     3121 agaaaaggta agaaaatgct ccttcaaaaa tgaaagaagg agttttttct ttaactgtac
     3181 ctcagtacgc aagagtggtg atacctcaag gaagagaata tttcctttta ccattatctg
     3241 atttttttgg agtggcacac cgagtttttc aataagtagt gaaactattt tttcagaatc
     3301 gtgctcagaa actttcagcc gctcctgaat tgttttaaga atattctgaa acgcgttcat
     3361 actagcgcta ccgattgagc ggcataagca gatagaggaa gttgtgatca gacacactct
     3421 ttactactag tgcccgattt ggctgagtaa gctcaatagc gatactatcg cccgagagtg
     3481 cttgaaacgc gtctaaaaag taccggaggt taatggtcac ctcaatcggt tcacccttga
     3541 gtaccgcatc aacactactc gtattttctc ccacatcagc attctgcgag gtaactgaaa
     3601 cagttttttc tgccggattt aaagaaaaca ttgtctgctg aaatttatcg gcaaaaatag
     3661 ttgagagccg cagtgtatta cttaaatctt gttttaagag gacacactgc gttttccact
     3721 cttttggaat aatctggcgg tagtcaggga atattccgtc aacgagccgt gaagtgagga
     3781 agataccacc ccctgaaaat gaaataagat ttttagtaaa ttcaatccgc acctcgccgt
     3841 tcaccccttc aagcacccga gtaatttctg cgatgttttt aaaaggaatc agtagttggg
     3901 ttatttcagg cacctgtttt agtttcagct ttttttctgc tagtcggaat gagtcggttg
     3961 ccacaaatac aatatgctca gcatcggtgt atataaaaat ggctgaaatt tcaggcttaa
     4021 tgtccgagac tgctgctgca aatgaaactg ctttaattcc ctgcacgagc acttttggat
     4081 caatagaaaa tactattccc tcttgcactg ccggaagggt ggggaagtct tcagttggca
     4141 ggcatttcac taatagtttt gacttctttg tttttaatac taagttttcg ttttcaagag
     4201 taagagtaat ttcatcacta tcagccaagg ttcccataat gccagcaatt acttcccctt
     4261 ttacgagcac taccccctct gtttcaattt tcgccggaat ttcaaactca attcctaaac
     4321 tcaaatttgt cgctcgaatt ttaagtgttt ttccggatgc aattaaaaga agtgctccga
     4381 gtactgggag ggtgaggttt tttccggtga ttcgctcaca gaggataatt gcgtttttca
     4441 gtttttctcc ggtgcatact actttcatat ggtttatctc tataagactt attaatacta
     4501 ctataggctg tggatttgtg gatagctcta ttttacctta tagagaaata atttttttat
     4561 attttttctc tctataactt acgtttagaa tccacttcat ccactttttt tcttaggtat
     4621 aaagaacgct gttttttata ccactctttt ccccactcca atccacctgt tttccctact
     4681 taaataacac gcggatgtct tcaagttcgc gcatgaggag tggattagtg aggagatctc
     4741 gttttatttt ttcgtacgag tgaataacag tggtgtggtc cttccctccc agttttgcgc
     4801 cgatagttgg atatgaggca ttaaaatctt cgcgcaggat aaacataatc atttgtcggg
     4861 cgcgaacaat ctctttccgg cgggtttttt cgtagatgat tttttcatca acgttgtagt
     4921 agttcgatat aatacggaca atatcctgca cgagtacatt tttcttaggt tttactgaat
     4981 atttaattag ctgcttaacc tcatcaaggc tgatttctcg atttttgagc tgtacatgac
     5041 aagaaattac atttaacacc ccttctagct cacgaatact actcgtgatt gaagttgcaa
     5101 ggtaggcaag aatgtcttca tttagttgga tattcattcc tgatgttttt tctcgcagta
     5161 ttgcaagacg ggattcgaaa tctggctcct gcacatcaat agtcattccg gcgttaaatc
     5221 gtgtttttaa gcgatcttca atccctaaaa tgtggtttgg gtgcttatct gaagaaaaaa
     5281 caatgttttt attctgatcg tagagggtat tgaagaggtg gaagagttct tcttgagttt
     5341 tttcttttgt tgagaggaat tggatatcat ccatgattaa gaaatcgtat tttcgatact
     5401 tttcacgaaa gctcgtcgcc cgattgttct gtaccgcact aatgtagtcg ttcgcaaatt
     5461 tctctaaggt agtatagaga accttttttt cagggaattt tgcttttatc tcattaccaa
     5521 tcgcctgaat gaggtgtgtt ttaccgagtc ctgagctccc ataaataaag agcgggttat
     5581 atgcctggct tgggcggcta atgaccgcct gggctgctgc ataggcaagt tcgttaaatg
     5641 agccaacaat aaagcggtca aacgtgtact tcgggttaag attatcttct ttatttatat
     5701 ataaatcttg gaatgggagt gcccccttcc cctcaataac tggcttctgt tcgctccgag
     5761 actcaagttt actgatggta aactcaatcg cacgtacatg ctgtgcctga tcgacaatcg
     5821 tttttaaaat aagtttgtga tatttgttga agagccactc tttgacgaac tcatttggta
     5881 ccccaatcac cagtaccccc tcttcttctc gtaaaatagt ggtattttta aaccaggtac
     5941 tgaagttagc gcgagtcacc tgcgattcaa tctcgcggag acatgtttgc cagagctggt
     6001 agagttcagt atccatgtgt attcgtaatc atacaggttt ttttagaaat gattcatctg
     6061 ttgagaacct gtgaatagtg tgaattgtat accaagttct gtatcttgtg aaagaagcgg
     6121 taaaagttgc aattatagta ctttttggta tagtgatttc acttatgtca tttacctaca
     6181 agccaaaaaa gaaaaagcgg gcaaaggcgc acgggtttct ctcgcgcagc aagtcaaaag
     6241 gaggtaagaa ggtactcaag gcgcgccgca gaaaaggtcg ggcaacgata gcagtctaag
     6301 cactatgctc ccgaaaaaga ataagctgcc acgggcagca ttcagtgaac ttctcgctca
     6361 gaatccaatt tctgtgttta atcgagttgg aacgctcaaa tatataccgc acagcggctc
     6421 attttcggtt gtggtatcag caaagcattg taaacgagct gttgctcgaa ataaggtgaa
     6481 gcgccgcgta tatagtctta tacaagtttt taagaaggaa tacactactc ccctctctgg
     6541 aatactctat ctttcaaaac acgcctacat gatgccattt tctgaattaa aaatattggt
     6601 tcaagaatta ctccgccgcg cctatgcgaa tagtaaatag tgctgctata tttttaatcc
     6661 atatatatca agctattttt tctcctgcga ccggaatatt gcggtttatt cctttttacc
     6721 ctaaacccag ctgtattttt tacccaacct gctctgaata tgcagtgtta gcctttagaa
     6781 aatatggttt tttgaaggca tttttcatct cgacaaagcg gattatgcgc tgccaccccg
     6841 ggaatgagcc aaaaattgat tatccgtagt tttcccgtac aatagaggac tgtgaagtca
     6901 ctctggaata ccctcatcta ccatccgctg ttgaacatac tcgttgcctt ggcggcagtc
     6961 atgcccggaa aaagccttgg tttagcggtt attgtgctca ccatcctcat tcggcttctc
     7021 ctcttcccta ttagtaagcg tgctattcga agccagtatg cgctccgagc gctcgagcca
     7081 aaagttcagg cgattaaggc aaaaaagctt gataaacaag aggaatcaag ggcgctcttt
     7141 cagttataca aagaagaaaa ggtaaacccg ttctcgggct gcctactgct ccttattcaa
     7201 ctccctatcc ttattgcgct ctattacgtg tttattaacg gcataaaccg ccccgaactg
     7261 ttgtactcgt tcattagtcc tgaaggaata cagcctactc tttttggttt aagcatcact
     7321 gctccatcga ttgtgctggc aatttttgca gcacttaccc aggcagtgca ggcatttctt
     7381 actccaaaac ctccaaaacc agctgagagc gataagataa actttcaatc acaacttgca
     7441 tcgagcatga gcatccaaac ccgctatgta ctcccgatta ttatattctt tatcgcgcgc
     7501 aatcttgctg ctgcggtgtc gctctactgg gtcgtgtcaa acctcttctc gattgggcaa
     7561 gagatttact tccgccggct cttcaaacat aaagcactta taaagcagtc ttaaaaagga
     7621 ttcctgattg tttgtctata aagtaaagag tttttgttac gctgctgtac tggggtgcgg
     7681 tgatatcaaa gactccagaa actgctttta gctgtgtttg ccccgaacta gggttaataa
     7741 cgacgaggtt gtctgaaaaa tggactctgc ctcggtacca gctttcaggg tatcctgcag
     7801 taggaatatt ttttggggtg gcgcagataa aggtttgatc agcagtccag acacattttt
     7861 cagggagaac gggtacggtg agtggcttcg tttctcctgt ttttgtattg aagagtgagc
     7921 tggtaaaact ggatccagta gcagtagcgc ttacgatgta ctgcccagca gggtttactg
     7981 cagcggtaag tccagggata ctgatcataa tttttttgta atcatttttt gaaagctgaa
     8041 acagtgagcc ggcaaagctt gaatctgctt ttgtttgcag gtacagctcg agccgggatg
     8101 cgagcaggag ccagtcttta aaagggtatt tgaagaacgc ctgctctttg ctgccgtcag
     8161 ggtttgagat tctgactaca ctctcgcctt gctcgtcggt gagtgttgcc agctgcttgc
     8221 cgtcagcaaa cactgccatt gaagaaatgt tgtctcgcaa gaagg
//
LOCUS       DATIBQ010000068         9247 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U16294
            k141_1553618, whole genome shotgun sequence.
ACCESSION   DATIBQ010000068 DATIBQ010000000
VERSION     DATIBQ010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935789
            Sequence Read Archive: SRR25158331
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 9247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:58:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 637
            CDSs (total)                      :: 603
            Genes (coding)                    :: 601
            CDSs (with protein)               :: 601
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9247
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1553618"
                     /isolate="SMAG_U16294"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..891
                     /locus_tag="VLB02_02225"
     CDS             <1..891
                     /locus_tag="VLB02_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014454085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HSE56878.1"
                     /translation="KSSFLYDVLYKNLQGRFERKHRTAKIFNCASITGLEYLQRAVLI
                     DQSPIGRTPRSNIATYTGAFMHVRDLFAATEEARLRGWKLNRFSFNVKGGRCEACEGN
                     GEIAVEMHFLPTVYVTCDVCGGKRFDKETLQVKFKKKNIYDVLQMTVEEAFTFFEDVP
                     AIYDRLKTLLDVGLGYVKLGQSATTLSGGEAQRVKIASELYRPWMEKTIYLLDEPTVG
                     LHYDDVKNLIAVLNRLVSKGNTVLVIEHNLDLIKCADWIVDFGPEGGERGGKIIAKGT
                     PEDVANNTKSFTGKYLKKVL"
     gene            985..5340
                     /locus_tag="VLB02_02230"
     CDS             985..5340
                     /locus_tag="VLB02_02230"
                     /inference="COORDINATES: protein motif:HMM:NF019311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HSE56879.1"
                     /translation="MSILKKLLVTIAVVAVGVFSFSNTATAQTVQKKFLIDLGAESVM
                     TPSWNNLTNGSVGAKIANLVDSTGVSSGIGLTVTDSFWQGWVGAYNGNGTTQSSLYPS
                     SATSDSLFVGNHLGTLDDTAKLRFSGLNQSATYTLRLFGSRMTDDQTSDRKTVFIVNG
                     QSKELQTKNNTSNVAEFTGLTASSGTLDLAVTLKTGSIYGYLGVIELIETATTTTPPP
                     STYTITASTGTPVITLPTNSVLLQSTSNAPGVGTWSQVSGPNTAVISTPTTAWTTVTG
                     LIQGSYVFRMTVRDYAGVVLGSADVSVTVNGNGTPPPSNVAPTANAGIDQIITLPTSS
                     VTLTGSGADSDGSISSYAWTKVSGGAATIGTPTAAVTSITSLAEGAYTFRLTVTDNGG
                     LTASDDVNVTVNAAPTPPPTGTGLAYKYYSYVPATAWVTPPQLPNLDTLTPVTTGTVT
                     KVDITPRLAEDTFAFRYEGALSVPTTGQYTFYLYSDDGSKLWIDGTLVLDHDGAHWAW
                     ERAATPVTLAAGTHSVKVEYYEGYGAQALMLSWQGPGIAKQEVPASAYSSTTTPPPPA
                     ENQPPTVNAGSDQIITLPTASVSLGATASDPNGDAMTYTWTKVSGGSATITSPSALAT
                     TVTGLAQGSYVFRFTATDSKGASAFDEVNVTVNGTVNSAPIVNAGTDQVITLPTSSVT
                     LSATASDPNGDAMVYSWTNVYGGTATITTSNALATTVTGLVQGGYTFRLTATDSKGAT
                     TADDVVVTVNAAPAVGLPPVVNAGTDLNVPEQTNSVNLTQSVSDPEGKTVTSSWAQVS
                     GPSTAGLYWQQWGVAAAYNLVKGTYVFRLTGTDADGASATDDVTVTVSASTGAGSPFM
                     RTVAQKSVVSNSKTIYYYESLPKGYNTDTTRKWPLIIYHHGIGERGSTLQSLPAILNQ
                     FPIGTSNLEYTVNGVNDSFIVMVPQLHETYSSWQNFFTQAMIDAAKSGLRVDTNRIYL
                     VGWSLGGFQTWNFPQGSDTNAAQIAAIAPVAGGSSGPSLCKIATNKIGVWAFHAVDDS
                     TVSVSTTDMAINNLNLCNPAPSPTPKYTRYTSGNHWIIGWVTDPNAPSGSNLYQWLLT
                     QSKTITTNTPPTANAGSDAIITLPTNSLTLSGSGTDTGGSISAYSWTKVSGGSATIST
                     PAAASTAITGLVQGSYTFRLTVTDNGGLTASDDVNVTVNAAVTGLNPAARTLTTRMTS
                     LPYLYNTPVGFYESLPRGYNNDTNRKWPLMIFLHGIGERGNGTTELPKLLSGNAVGSK
                     IAAGEQLEYNVNGINDSFVVMMPQLSTSASDWHPYHVRGMIDYAVANYRVDPKRIYIT
                     GLSLGGFGTTAYPEFSTAFANQIAAVAPTDGANYGTSIWTPDLGSITYNTCNLVAAGV
                     KVWQFYGADDTSWGWTAANFINRLNACNPAPNPAPLVTVYPGVGHWAWGNTYDTTHNI
                     QNPNVYEWLLAQKRP"
     gene            5424..6668
                     /locus_tag="VLB02_02235"
     CDS             5424..6668
                     /locus_tag="VLB02_02235"
                     /inference="COORDINATES: protein motif:HMM:NF020048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HSE56880.1"
                     /translation="MIRQDLDKSNLPITPGVYFFKDAAGKILYIGKATVLRDRVRSYF
                     SPDVIKTRGAHIVDMVFKASKVDFEEAGSVLEALMLEANYIKKWQPYYNTKEKDDKSY
                     NCVVVTKELFPRVLLVRQKDSDAKAKEVYVPKSGTRLKYDTLYGPYPNGSAIKEALRI
                     IRRIFPYLDTASLQKDKVAFYRQVGLSPDTRDREAQARYKETVHHLKLFFSGKMKVLR
                     ESLTKDMQAAAKAERFEEAQQYKEQLFALDHIRDVSLIKRDLFAAGADTTFRIEGYDV
                     AHLSGKNMVGVMVVADNDRMVKSEYRKFTIKSVSGANDPAALTEMLERRLNHHEWQLP
                     NLIVVDGNTIQRNAAQAVLLRRRLQIPVVSVVKDERHKPKEISGEKSLIEMHKLGILL
                     ANSEAHRFALSFHKEKRKKQML"
     gene            6678..7652
                     /locus_tag="VLB02_02240"
     CDS             6678..7652
                     /locus_tag="VLB02_02240"
                     /inference="COORDINATES: protein motif:HMM:NF019121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56881.1"
                     /translation="MSHRIGVIRGGTSSEYPISLKTGAAVLNALREEGYEAIDILLDK
                     DGILHIKGIPATLEQLPHHVDLAWNALHGHFGEDGKLQQLLEATGVPYTGSRALASAM
                     GANKAVAKETARSLGLQVPPSLLVNPAEESDSVAAITQRIYRTMAPPWVVKPLSGGSS
                     INTYLVHTPLELSQIVDEAMLRGELFLVEQYIYGREANVSIIDGFRGQEHYGAPVVEV
                     QKPGRGIFTHEMKYGGSEPQARVGGSFRADERDVLSRHAKAMHELLGAEDVSQSEFII
                     NPYGKIYFLEIDTNPALTDTSLLPLSLQSVGSSLRELIAHIVKRRLGS"
     gene            7717..7789
                     /locus_tag="VLB02_02245"
     tRNA            7717..7789
                     /locus_tag="VLB02_02245"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7750..7752,aa:Ala,seq:tgc)
     gene            7967..8608
                     /locus_tag="VLB02_02250"
     CDS             7967..8608
                     /locus_tag="VLB02_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004481069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4145 domain-containing protein"
                     /protein_id="HSE56882.1"
                     /translation="MDQKMEIIECPYCESRVSAKILALKDGNSEEDGSPVRILFLECS
                     ICNHVLLGYSEITQIDSNDWVFITPERLWPNPKNVLSWKLPYLVRSSIEEAKACLGAR
                     AYSACAVMCGRALESVCKEHSIKNWKLQNGLKELKEQGIIDGRLFDWGEELRKSRNIG
                     AHAVEGKITKEDAIDLLDFTIAICDYVYVLSDKYKSFKERQEKRKKTITIKEK"
     gene            8970..>9247
                     /locus_tag="VLB02_02255"
     CDS             8970..>9247
                     /locus_tag="VLB02_02255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56883.1"
                     /translation="MASSAQQKDSYNRDYLRQVEKYVSLLKEPFKNFPNNPMFEVHSV
                     ADMKDHEKKEGSLQILSKFEDAVVVVHFPDSPLGLGGYESNLNNRGLLL"
BASE COUNT         2639 a         2056 c         2149 g         2403 t
ORIGIN      
        1 aaaagcagct ttctctatga cgtgctctat aaaaacttgc aagggcgatt tgaacgcaag
       61 catcgcacgg caaaaatttt taattgcgct tcaattactg gtttagaata tctgcagcgg
      121 gcggtactga ttgatcaatc accgatcggg cgaacaccgc gctcaaacat tgcaacgtat
      181 accggtgcat ttatgcatgt tcgcgattta tttgccgcaa ctgaagaagc acgcttgcgc
      241 ggctggaagt tgaaccgttt ttcatttaac gtaaaaggcg gtcgttgtga agcgtgcgag
      301 gggaatggcg agatcgcggt tgagatgcac tttctgccaa cggtttatgt tacctgtgat
      361 gtctgcggcg gcaagcgatt tgataaagaa acactccagg taaaatttaa aaagaaaaat
      421 atttacgatg tactgcagat gaccgttgaa gaagcgttta ctttttttga agatgttcca
      481 gcgatttatg atcgtcttaa aacactcctt gatgttggct tgggctatgt aaaacttggg
      541 cagagtgcaa ccaccctttc aggcggagaa gcgcagcggg taaaaattgc ttcagagctg
      601 tatcggccat ggatggaaaa aacaatctac ctgcttgatg agccgacggt ggggctccac
      661 tatgatgacg taaaaaattt aattgccgta ttaaaccgat tagtttcaaa agggaacacg
      721 gtgttggtga ttgagcataa tctcgattta ataaaatgcg ctgattggat tgttgatttt
      781 ggccccgagg gtggagagcg cggcggtaag ataattgcta aggggacacc tgaagatgtg
      841 gcaaacaata caaagagttt tactggaaaa tatctgaaaa aagttctcta aaatgcacca
      901 gtggataacc aaggatagct aatttgacga tttggtaata aaataggtgg atattagtaa
      961 ggtagagata attgtagagc atatatgagt attttaaaga agctgttggt aaccatcgct
     1021 gttgtggctg ttggtgtttt ttcgttttca aatacagcca ctgcacaaac agtgcaaaag
     1081 aaatttttaa tcgatcttgg cgctgagtca gtaatgaccc caagttggaa taatcttact
     1141 aacggatcag ttggggcaaa aattgcaaac cttgttgatt caacaggggt aagttcaggt
     1201 atcgggttaa ccgttaccga ttcattctgg cagggatggg taggtgctta taatggaaac
     1261 ggtaccacgc agagttcgct ctacccaagc tcggcaacaa gcgactcgct ctttgttggt
     1321 aatcaccttg gtacactcga cgataccgca aaacttcgtt tctctgggtt gaatcagagt
     1381 gctacctaca cccttcgctt gtttggttct cgtatgactg atgatcaaac ctctgatcga
     1441 aaaacagtgt tcatcgtaaa tggtcagtca aaagaattac agacaaaaaa taacacttct
     1501 aatgttgctg agtttaccgg tcttaccgca tcaagtggaa cgctcgattt ggcggtgaca
     1561 ctcaagactg gatcaatcta cggatacctt ggtgttatcg aacttattga aactgctact
     1621 acaacaaccc ctcctccttc tacctacaca ataactgctt ctaccggaac tccagttatt
     1681 actctcccca caaacagtgt tcttttgcag agtacttcaa acgctcccgg tgttggaaca
     1741 tggagtcaag tatcgggacc aaacactgca gttatctcta ctcctaccac cgcatggaca
     1801 acagtaacag gattgataca aggctcatat gtctttcgca tgacagtacg agattacgca
     1861 ggtgtcgtgc tgggaagcgc tgatgtttca gtaactgtaa atggtaatgg aactcctcct
     1921 ccaagtaacg tagctcccac tgctaatgca gggatcgatc aaataattac cctccctact
     1981 tcatcagtaa cacttactgg ttcaggtgct gattccgacg gttctatcag tagctatgca
     2041 tggactaaag tttctggcgg tgcagcaact atcggtactc caactgcggc tgttacttca
     2101 attaccagtc ttgctgaagg cgcctacacc ttccgcctaa cggtgaccga taatggcggc
     2161 ttgactgcaa gtgatgatgt gaatgttaca gtgaatgcag caccgactcc accaccaact
     2221 ggtactgggc ttgcgtataa atactactca tatgtgccgg ctaccgcttg ggtgactcca
     2281 ccgcagctgc caaatcttga tacgcttact cctgttacaa cagggactgt taccaaagta
     2341 gatattactc cacgattagc agaagatacc tttgcctttc gttacgaggg ggcgttgagc
     2401 gtgccaacta ctggtcagta cacgttctat ctttactctg atgatggatc aaaactctgg
     2461 attgatggca cactcgtact tgatcacgat ggagctcact gggcatggga acgggcagca
     2521 actccagtaa cgctcgctgc cggtactcac tcggtaaagg ttgagtacta tgaagggtac
     2581 ggcgctcagg cgctcatgct ttcgtggcaa ggtccaggaa ttgccaaaca agaggttccg
     2641 gcgagcgcgt attcttctac cacaacacct ccaccacctg ctgaaaatca gccaccgaca
     2701 gtaaacgcag ggagcgatca aataattacc ctccctaccg cttcagtttc gcttggtgca
     2761 actgcgagtg atccaaatgg tgatgcaatg acctacacct ggacaaaagt ctctggcggc
     2821 agtgcaacaa taacgagccc gagcgctctt gctaccactg ttaccggttt agcacaggga
     2881 agctatgtat ttcgcttcac cgcaactgat tcaaaaggtg cgagtgcttt tgatgaggtg
     2941 aacgtaactg taaacggcac tgttaatagt gctccaatag taaacgcagg aacagaccaa
     3001 gttattaccc tccctacctc atctgtaaca ctcagtgcaa cggcaagcga tccaaacggt
     3061 gatgcaatgg tgtactcgtg gacaaatgtt tatggcggca cggcaaccat aactacctca
     3121 aacgcactcg cgactacggt aacagggctg gtacagggag gttatacgtt ccgtttgacg
     3181 gcaactgatt caaaaggtgc tactacagct gatgatgtag tagtaacagt aaatgctgca
     3241 ccagctgtcg ggttgccccc agtggtgaat gcaggcaccg atcttaacgt tcccgaacaa
     3301 acaaatagtg taaaccttac ccaaagcgta agtgatccag aaggaaaaac agttacttca
     3361 agttgggcac aggtttcagg tccaagcacg gcaggattat actggcagca atggggtgta
     3421 gcagcagcat ataacttggt aaaaggcacc tatgtattcc gccttaccgg aacggatgct
     3481 gacggggcaa gtgcgactga tgatgtaacg gttactgtga gtgcaagcac tggagccggg
     3541 tctccattca tgcgcactgt ggcgcagaag tcagttgttt caaacagtaa aactatttac
     3601 tactacgaat cactgccaaa aggctacaat accgacacta cccgcaaatg gccgctgatt
     3661 atctaccacc atggtattgg cgagcgtggc agtaccttgc agagtctccc tgcaattcta
     3721 aaccagttcc caattggaac atcgaatctt gagtacactg taaatggagt aaatgattcg
     3781 ttcattgtca tggtgccgca gctccacgaa acatatagca gctggcagaa cttcttcacg
     3841 caggcaatga tcgatgcagc aaaatcaggt ttgcgtgttg atacgaaccg tatctacctg
     3901 gttggctggt cgctcggcgg ttttcagaca tggaatttcc cgcaggggtc tgacactaat
     3961 gccgcacaaa ttgcagctat tgctcctgtt gctggcggct cttctggtcc ttctctctgt
     4021 aagattgcaa ctaataaaat tggggtgtgg gcattccacg cagttgacga cagtactgtt
     4081 tcagtatcaa caactgatat ggcaatcaat aacctcaacc tctgtaaccc tgccccttca
     4141 ccaacgccaa aatacacgcg gtatacctcg ggtaatcact ggattattgg ctgggtaacc
     4201 gatccaaacg ctccaagcgg ctcgaatctt taccagtggc tcctcacgca gagtaagacg
     4261 ataaccacta atacaccacc taccgcaaac gctggctctg acgctattat cacccttccc
     4321 acaaactcgc tgacactctc aggttcgggt accgatacgg gaggttccat aagtgcgtat
     4381 agctggacaa aagtctctgg cggcagtgca acgatcagta caccagcagc agcttccact
     4441 gctattactg gtctcgttca gggctcatac accttccgcc taacggtgac cgataatggc
     4501 ggcttgactg cgagtgatga tgtaaacgta accgtgaacg cagcggtaac agggctcaat
     4561 cctgcagcgc gaaccttaac tactcgcatg acaagcttgc cgtatctcta caacacccct
     4621 gttggctttt atgagtcact gccgcgaggt tacaacaatg atacaaatcg caagtggccg
     4681 ctcatgatct tcttgcatgg tattggcgag cggggaaatg gtactactga acttcctaaa
     4741 ctcttatcag gaaatgctgt tgggtcaaaa attgctgctg gtgaacagct tgaatataac
     4801 gtaaacggta ttaacgattc atttgtagtg atgatgccac aattgagtac atctgcttca
     4861 gattggcacc catatcacgt ccgtggaatg atcgattacg cagtagcaaa ctaccgagtt
     4921 gatccgaagc gtatttacat cacggggctc tcgcttggtg ggtttggaac cactgcatac
     4981 cctgaattct caaccgcatt tgccaaccaa attgcagcgg ttgctccaac tgacggcgca
     5041 aactatggta cgagcatctg gacacctgat ttgggaagca tcacctacaa cacctgtaac
     5101 ttagttgctg ccggggtgaa agtgtggcag ttctatggtg cagacgatac gagctgggga
     5161 tggactgcag caaactttat taaccggctc aacgcatgca atccggcacc gaaccctgct
     5221 ccactggtga ctgtctaccc aggcgttggg cactgggcat ggggaaatac ttatgacact
     5281 actcacaaca ttcagaatcc gaatgtctac gagtggctcc tcgctcaaaa gcgcccataa
     5341 gcttaagtta gaactcaaaa ataccccgac tggggtattt ttgtttagcg gttacatttt
     5401 ttctgatata ctgcaacgct agtatgattc gtcaagattt agacaagagt aacttgccca
     5461 ttacgcctgg tgtgtacttt tttaaggatg ctgcggggaa gattctctat atcggtaaag
     5521 caaccgtgct ccgcgaccga gtgcgaagct acttctcacc tgatgttatc aagactcgcg
     5581 gagcgcatat tgtagacatg gtatttaagg ctagtaaggt agattttgaa gaagcgggta
     5641 gtgtgctcga agcgctcatg ttagaagcca actacatcaa aaagtggcag ccgtactaca
     5701 acactaaaga aaaagacgat aagagttata actgtgtggt tgtcaccaaa gaattgtttc
     5761 cgcgggtgct gttagtgcgg caaaaagata gtgatgcaaa agcaaaggag gtatatgttc
     5821 caaaatcagg cacaaggcta aaatacgata cactgtatgg tccataccca aacggatcgg
     5881 cgatcaaaga agcactcaga attatccgcc gaatttttcc atacctcgac accgcatcgc
     5941 tccaaaaaga caaggtcgca ttttatcgac aagtaggact ctcgcctgat acgcgcgata
     6001 gagaggcgca ggctcgatat aaagaaacag tacaccactt aaaactcttt ttctccggaa
     6061 aaatgaaagt gctccgagaa agccttacta aagacatgca agcagctgct aaagcagagc
     6121 gatttgaaga agcgcagcaa tacaaggagc agctcttcgc gctcgaccat atccgggatg
     6181 tgtcgctcat caagcgcgac ctctttgctg caggagccga tacgacattc agaattgaag
     6241 ggtacgatgt ggcacattta tcgggtaaga acatggttgg ggtgatggta gtagcagata
     6301 acgaccggat ggtaaaaagc gaataccgaa aatttaccat taagagcgtg agcggtgcca
     6361 atgaccctgc agcactgact gaaatgttgg aacgccgact taaccaccac gagtggcaac
     6421 tgccgaacct gattgtcgtt gatggaaaca caattcagcg aaacgcagca caggcggtgc
     6481 ttttgcgccg ccgcctgcaa atccctgtag tgtcggtggt aaaagatgag cggcataagc
     6541 caaaagaaat tagtggagaa aaaagtctga tagaaatgca caagcttggc attttgcttg
     6601 ctaacagtga agcgcaccgg tttgcgctca gttttcataa agagaaaaga aaaaagcaga
     6661 tgctataata aaagcacatg tctcatcgta tcggcgttat tcgcggcggc actagctcag
     6721 aatatcctat ctcactcaaa acaggtgcag cagtattaaa cgcgctccgc gaggaaggct
     6781 acgaggcgat tgatatcttg ctcgataaag atggtattct ccacattaaa ggtattccgg
     6841 ctacccttga gcaactacca caccatgttg atttagcctg gaatgcgctc catgggcatt
     6901 ttggtgaaga cggcaagctc cagcaactgc ttgaagcaac tggtgttcca tatactggtt
     6961 cgcgtgcact tgcttctgcg atgggagcaa acaaggctgt tgctaaagaa acagcccgca
     7021 gtcttgggct ccaggtaccg ccctcgcttc tcgtgaatcc tgcagaagag agcgattcag
     7081 tggcggcaat tacccagcgc atttatcgca ctatggcgcc accgtgggtg gtaaaaccgc
     7141 tctcgggcgg gtcgtcgatc aatacttact tggtacacac cccgcttgaa ctctcgcaaa
     7201 ttgttgatga ggcaatgctg cgcggcgagc tttttttagt tgagcagtac atctatggca
     7261 gggaagcaaa tgtttctatc attgatggct ttcgcgggca ggagcattac ggagcgccgg
     7321 tggtcgaggt acagaagccg ggcaggggaa tatttacaca cgagatgaaa tacggcggca
     7381 gcgagccaca agctcgggtg gggggcagct ttcgcgccga tgaacgcgat gtgctctctc
     7441 gccatgcaaa agcaatgcac gagctgctgg gggctgagga tgtttcgcag tctgaattca
     7501 ttatcaaccc ctacggaaaa atctactttc ttgagataga caccaaccca gctcttaccg
     7561 acacttcact cctcccactc tcgctccagt cagtcggttc aagcctccgc gagctgattg
     7621 cgcatattgt aaaaagacgc cttgggtcat aaagaggatt ttaaaggagt tgcttttttc
     7681 cttatttctc gtaaactatc ttagttttca atatatgggc ctatagctca gttggtagag
     7741 cagcgctttt gcaaagcgaa ggtcaggagt tcgaatctcc ttaggtccac tactttggtt
     7801 tgaacacaat ttagcctctc tatacagaaa tagcctttta aaataaggtc cattttctca
     7861 aaataagcct ctttctgtgg aaaagtacca ctctggacaa actttgaact cttcaatttt
     7921 gaaaaaatcc tgttttaggg gtacagttaa ataagtttta tattttatgg atcaaaaaat
     7981 ggaaataatt gaatgtccat attgtgagtc tagagtaagc gcaaaaatat tagctttaaa
     8041 agatgggaac tctgaagagg atggctcacc agttaggata ctatttttag agtgctcaat
     8101 atgtaatcat gtcttacttg gttactcaga aataacccag atagattcta atgattgggt
     8161 ctttattaca ccggaaaggc tttggccaaa tcctaaaaat gtattgtcat ggaaattacc
     8221 atatctagta agatcttcta ttgaagaggc aaaagcatgc ttaggtgctc gtgcctattc
     8281 tgcctgcgct gttatgtgtg gtcgcgcact tgaatctgtt tgcaaagaac attctattaa
     8341 aaattggaag ttacaaaatg gtttaaaaga acttaaagaa caaggaatta ttgatggtag
     8401 attatttgat tggggtgaag agttacgtaa aagtcggaat attggagcac atgccgtaga
     8461 aggtaaaatt accaaagaag atgcaataga tcttttagat tttactattg caatttgtga
     8521 ttatgtatac gtgctttcag ataaatataa atcttttaaa gaaaggcaag aaaaaaggaa
     8581 aaaaaccata acaataaaag aaaaatgaat ctatttttag agtttctctc ctcatataca
     8641 ttagaagctt tccatctcca acttctcccc gaatataaaa ccccaggaga atgggaagta
     8701 tttcagcaat acaaagaagg cggtctatat acctattctt cagacttcaa agaagagttt
     8761 aaactccagg caggacagta ttctgtctta tggtatatct ccttgggttg tgtggttctc
     8821 actaactaca taaattgcga gtccgggaga aggggataac ccttttctcg aattttttat
     8881 ttcagaagct tctattgaca aatataattt tatgtgataa agaatagatg atttgctctt
     8941 aatttactga attaaaaaaa ttatagttta tggcatcttc tgctcaacaa aaagattcct
     9001 ataatcggga ttatctccgt caggttgaaa agtacgtttc tctattaaag gaacccttta
     9061 agaattttcc caacaatcca atgtttgaag ttcactctgt ggctgatatg aaggatcacg
     9121 aaaagaagga gggtagtctt caaatcttat caaaatttga agatgccgta gtggtagtac
     9181 attttcctga ttcgccgctt ggtttaggtg gctatgaaag taatttgaac aatagaggat
     9241 tacttct
//
LOCUS       DATIBQ010000069         4122 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U16294
            k141_878640, whole genome shotgun sequence.
ACCESSION   DATIBQ010000069 DATIBQ010000000
VERSION     DATIBQ010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935789
            Sequence Read Archive: SRR25158331
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 4122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4122)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:58:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 637
            CDSs (total)                      :: 603
            Genes (coding)                    :: 601
            CDSs (with protein)               :: 601
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4122
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_878640"
                     /isolate="SMAG_U16294"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..216
                     /locus_tag="VLB02_02260"
     CDS             <1..216
                     /locus_tag="VLB02_02260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56884.1"
                     /translation="IAVHSGYYYHDTRQYSEIEGSTAEVRIVSALVEGVVGEDMKKFL
                     AVQEILGKIMAEASALVTSVQKKEIVQ"
     gene            complement(258..503)
                     /locus_tag="VLB02_02265"
     CDS             complement(258..503)
                     /locus_tag="VLB02_02265"
                     /inference="COORDINATES: protein motif:HMM:NF013026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bL28 family ribosomal protein"
                     /protein_id="HSE56885.1"
                     /translation="MAKVCAITNKSSLVTGGYSNRTRATKYQPTGKSRKYANLQKKRI
                     FVPELNKTFVLTISTSGIRTINKNGAYAALKKAGVIK"
     gene            538..1161
                     /locus_tag="VLB02_02270"
     CDS             538..1161
                     /locus_tag="VLB02_02270"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HSE56886.1"
                     /translation="MQGIDGVFYVVILIISVIMHEVAHGLAADHFGDPTARLSGRLSL
                     NPIRHIDPFGSVILPLLLIFSGTGVVVGWAKPVPYNPNLMRNQRFGVPAVALAGIVTN
                     LCIALFFGIVIRLGMGSDFLSQGFFHIASTIVLVNLVLALFNVLPLPPLDGFRLIFSL
                     IPRRLRRYEQLSERYGIFLLILFILFGWRFVAPLVIFLYSFFTGIHL"
     gene            1169..2965
                     /locus_tag="VLB02_02275"
     CDS             1169..2965
                     /locus_tag="VLB02_02275"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HSE56887.1"
                     /translation="MRISLRAALFFSLLFFIALGVSASSSLGVIDPNGSGNYKAEILN
                     DPGYASPTINFGKFTTQSASNITVSSSELRGFAWGELTGWIVMNCADTSSGCSGTNGN
                     FKVANNGSGLLSGYAWGENTGWVNFGPFTSGAAQVKITSSGLFGGSVGTAGYAWSENF
                     GWIIFDCGNANTCVETDWRQGGGNSSGSGGGGGGDGNPPPTPPPTPPPSPPVIPPETP
                     LPPPDTVPPAPPIEPEPPIPGGDSGPGAPGGGGGGGSPPNREGFRLPTLPKDIPGIVT
                     KQFERATKVVVETLAIVATVLDTPVGQATVAVTASAGMLAFFLQTLFSNGWRFFLSLF
                     GFYRKRRNWGTVYDSVTKQPLDPAYVVVYDKFGREVVTQITDLDGRFGFLLPPGEYTV
                     KANKTHYAFPSTVLAGKTSDGIYNDLYFGEPITIANKEQVVIKNIPMDAQAFDWNEAE
                     KRRRNLFYFTRSNAFSKISIIVFSIGFAISFLALVLAPSVGHLVVFGVYVLIYLIKTF
                     FIPPVTSSFVLEKSTRKPVPFALVRIYGANSRSELMKKVTSLTGGFYCLIANGTYFLT
                     VERRNPDGTYTLAHTSSVFTVKKGVIAAEIEI"
     gene            2962..3768
                     /locus_tag="VLB02_02280"
     CDS             2962..3768
                     /locus_tag="VLB02_02280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HSE56888.1"
                     /translation="MKFSLRKKKAAEWGTVYDSVTKQPLDPVYVTVYDMVGREVATSI
                     TDLDGRYGFVLAPGMYRIVVGKTHYEFPSKRLAGKTKDEVYRDLYFGENIVITEAEQV
                     ISQNIPMDPLAVDWNETEKQRRNLGYFRGGGFEWLVTGLFALGFLIALIVFVLAPNRL
                     HLMVFGAYVLLYFIKVLTSGEKKRSSLVIHSATREPVPFALVHVYAANDKREVTKKVT
                     SYTGTFFSLIPNGNYFVTIETRNPDGTYTLAYTSSVFTVVRGMISKTFAI"
     gene            3851..>4122
                     /gene="rpsL"
                     /locus_tag="VLB02_02285"
     CDS             3851..>4122
                     /gene="rpsL"
                     /locus_tag="VLB02_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HSE56889.1"
                     /translation="MPTLNQLIRNKRSKPKRKSKAVALSRGFNAIKNKPVFYPAPFKR
                     GVCVKVSTTTPKKPNSAIRKIARVRLTNSQEVTAYIPGIGHNLQEHS"
BASE COUNT          987 a          966 c         1040 g         1129 t
ORIGIN      
        1 attgcggtac actcagggta ttactatcat gatactcgac aatattctga gatcgaagga
       61 tctactgctg aggtaagaat tgttagtgcg cttgtcgaag gggtggttgg cgaggatatg
      121 aaaaaatttc ttgcagtaca agaaatacta gggaagatta tggcagaagc ttctgctctg
      181 gtaacttctg tccagaaaaa agaaattgta cagtaaaaaa gccgtctcgg tatataccga
      241 gacggctttt ctttttgtta cttaattacc cctgcttttt tgagtgctgc gtaggcgccg
      301 tttttgttga tggtgcggat gcctgaggta gaaatggtta atacgaaagt cttgttgagt
      361 tccggcacaa aaatacgctt cttttgaagg tttgcgtact tgcggctttt gccagttggc
      421 tggtacttgg tagcacgagt gcggttcgag tagccgcccg taaccagaga agatttatta
      481 gtgatagcgc atacttttgc catagacagg ggagatgcta ccatacacag tactcccatg
      541 caaggcatcg acggcgtttt ctatgtagta atcctcatca tttcggtgat tatgcatgaa
      601 gtggcgcacg gcttagcagc cgaccatttt ggcgacccga ccgctcgact ctcagggcgg
      661 ctctccttaa atccgattcg tcatatcgat ccgttcggtt cggtgattct tccgctcttg
      721 cttattttct ccggaacggg tgtggtagtt ggctgggcaa agccggtgcc ctacaatccc
      781 aacctcatga gaaaccagcg ctttggcgtg ccggcggtgg cactcgctgg tattgttact
      841 aacctgtgta ttgcgctctt ttttggaatc gttattcgct taggaatggg gagcgatttt
      901 ttgagtcagg gattctttca tattgccagc acgattgtgc ttgtgaattt agtgctcgca
      961 ctctttaacg tactgccgct gccgccgctc gatgggtttc ggcttatctt ttcacttatc
     1021 ccgcgccgct tacgtcgtta tgaacaacta tctgaacggt atggtatctt tttgctcatt
     1081 ttatttattc tctttgggtg gcggtttgtt gcgccgttag ttatattttt gtattccttc
     1141 tttaccggca ttcatctata atgtacgcat gcgaatctcg ctccgtgcag ctcttttctt
     1201 ttccctcctt ttttttatcg cgctcggtgt ttctgcatcg agcagccttg gagtcattga
     1261 cccaaacggt tcaggtaatt acaaggcaga aattttaaat gatccggggt acgcaagtcc
     1321 aacaattaac ttcggaaagt ttactacgca gtctgcttca aatatcacgg tctcaagcag
     1381 cgagctgcgc ggctttgcct ggggcgagct taccggatgg attgtgatga actgtgccga
     1441 tacctcgagc ggctgtagtg gcacaaacgg aaactttaaa gttgcaaaca atggttcggg
     1501 gctgctctcg ggatatgctt ggggagaaaa cacgggctgg gtgaattttg gtccgtttac
     1561 gagcggtgct gcacaggtaa aaattacctc tagcgggttg tttggtggtt ctgtaggaac
     1621 tgcggggtat gcctggagtg aaaattttgg ctggatcata tttgattgcg gcaacgcgaa
     1681 cacctgtgtt gaaactgact ggcgccaggg cggcggcaac tcaagcggta gtggcggcgg
     1741 aggtggggga gacggtaatc ctcctccgac accaccgcct actccgccgc caagccctcc
     1801 ggttattccc cccgaaacac cactgccgcc acctgatact gttccgcctg ctccaccgat
     1861 tgaacctgaa ccgcctatac ctggcgggga tagtggtccg ggtgcacctg gaggcggcgg
     1921 tggtggcgga agtcctccaa accgagaagg ctttcgtttg ccaacactac caaaagatat
     1981 accaggtatt gttactaaac agtttgagcg agcaacaaaa gtagttgttg agacgcttgc
     2041 tattgttgca acagtactcg atactccggt cgggcaggca actgtagcag ttactgcaag
     2101 tgcgggaatg ctggcattct ttttacaaac cctgttcagt aatgggtggc ggtttttctt
     2161 gagcctcttt ggtttttacc gaaagcgacg caactggggc acggtctatg attcggttac
     2221 caaacagccg ctcgatccgg cgtatgtggt ggtttacgac aaatttggca gagaagtagt
     2281 aacacaaatt accgatcttg atggtcggtt cggcttcttg cttccgcctg gagaatacac
     2341 ggtgaaagcg aacaaaaccc actacgcgtt tccttcaaca gtgctcgccg gaaaaacgtc
     2401 agacggcatt tacaacgatc tctactttgg cgagccaatt acgattgcaa acaaagagca
     2461 ggtggtgatt aaaaacattc cgatggatgc gcaagcgttc gactggaacg aggcagagaa
     2521 gcgccgccgg aacctcttct acttcacccg cagtaatgcc ttcagtaaaa tttcgatcat
     2581 tgtgtttagt attgggtttg cgatttcatt cttagcactt gtattggcgc caagtgtcgg
     2641 gcatctggtg gtgtttgggg tttatgtact catttacctg ataaaaacat tcttcatacc
     2701 gcccgttacg agctcgtttg tgcttgaaaa gtcaactcga aaaccagtac cattcgcgct
     2761 cgttcgtatc tatggcgcaa acagtcggtc ggagctgatg aaaaaagtta cctctctgac
     2821 gggaggattc tactgcttaa tagcgaatgg cacgtacttc ctcactgttg agcggcgcaa
     2881 ccctgatggt acctacaccc ttgcacatac ctcaagcgtg tttacggtaa agaaaggggt
     2941 gatcgcagct gaaatcgaaa tatgaagttt agcttgagaa aaaagaaggc tgctgagtgg
     3001 ggcacagtct acgattcagt taccaaacag ccgctcgatc cggtgtatgt aaccgtctac
     3061 gatatggtcg ggcgcgaggt ggcaacttca atcactgatc ttgatggtcg gtacgggttc
     3121 gtgctcgcgc cggggatgta ccgcattgta gtggggaaga cgcactacga atttccctca
     3181 aagcgccttg ctggaaaaac caaagacgaa gtgtaccgcg atctctactt tggcgagaac
     3241 atagtaatta ctgaagccga acaggtgatt tcacaaaaca ttcccatgga tccgctcgct
     3301 gttgattgga acgaaacaga aaagcagcgg cgcaaccttg gttactttcg cggaggcggc
     3361 tttgagtggc tcgttaccgg actttttgca cttggatttt tgattgctct gattgttttt
     3421 gttttggcgc caaaccgtct gcacctcatg gtgtttggcg cgtatgtact gctttatttt
     3481 attaaagtgc tcaccagcgg agaaaaaaag cgttcgtcgc tggtgattca ctctgccacc
     3541 cgcgagccag taccattcgc gctggtgcat gtatatgcag caaatgataa gcgggaagta
     3601 accaaaaaag taacctcata caccgggacg ttcttttcac tcattccaaa tggcaactat
     3661 tttgttacta ttgaaactcg gaaccctgac ggtacctaca ccctggctta cacttcaagc
     3721 gtctttacag tagtgcgggg gatgatctca aaaacctttg cgatttagcc aggactagtg
     3781 tgcttgcaat ttattcacac ctgtattacg ataacgctca caaggctttc tgcctcgttt
     3841 ttatttaaag atgccaaccc tcaatcagct catccgcaat aagcggtcaa aaccaaaacg
     3901 taaatcaaag gcggtggcgc tttcgcgcgg ctttaacgct attaaaaaca agcccgtctt
     3961 ctacccggca ccgtttaagc gcggggtgtg cgtaaaggta agcactacca cgccaaagaa
     4021 accaaactca gcaatccgaa aaatcgcccg tgttcgcctc acaaatagcc aagaagtaac
     4081 ggcatatatt ccgggtattg gacataactt gcaggaacac tc
//
LOCUS       DATIBQ010000070        13741 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U16294
            k141_2041574, whole genome shotgun sequence.
ACCESSION   DATIBQ010000070 DATIBQ010000000
VERSION     DATIBQ010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935789
            Sequence Read Archive: SRR25158331
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 13741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13741)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:58:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 637
            CDSs (total)                      :: 603
            Genes (coding)                    :: 601
            CDSs (with protein)               :: 601
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13741
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2041574"
                     /isolate="SMAG_U16294"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..523
                     /locus_tag="VLB02_02290"
     CDS             <1..523
                     /locus_tag="VLB02_02290"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HSE56890.1"
                     /translation="WLRSQIGNVEQKIDLFDRSIRDNILFGLPGDSQISDEKLQKVIS
                     DASLDDFIAKLKDHGLDTMIGESGIKVSGGERQRIGIARALIKDPKILIFDEATSALD
                     SINEKLIHDAINRSAKGRTTIIIAHRLSTVMDADIIFVVADGKIVDSGTHKILEKRCK
                     EYKNLIKNQILVA"
     gene            590..1672
                     /locus_tag="VLB02_02295"
     CDS             590..1672
                     /locus_tag="VLB02_02295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56891.1"
                     /translation="MEKLLKALNKLVFLTPRTETDIQTIQKLAIEIQKDFVFSTYWLG
                     LIDNYTEQFKKPNFKSAIRRAMWAATHYVRPGSGFTPKLSTIEDDKSLGYNCLGRAIM
                     LGSNLRYCKYKFKVFLGITPDHAMVLIELKNTLYLCDSLAMKLRKLHGTCNTHEGYRW
                     YLGSPEDEFMNNYLVIHEFDRGIVNAIVESFIFLQEPPQNPSGAEQEFLVFKDPRFKL
                     HMAINQLCWRDIQKKYFSDLNEYRNRYRKEYLLECVFIEKQRHVQNLRAQFNQVTRSA
                     YEKAFKSPYDNEKHKSFIVESTFILQSIHEHVIRFLEGNVSALDLPVQLGIYLQTIRE
                     RVGNNAELQQFTIDKFKYRMSTQISI"
     gene            1693..1854
                     /locus_tag="VLB02_02300"
     CDS             1693..1854
                     /locus_tag="VLB02_02300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56892.1"
                     /translation="MKAEQLLEDGKSIMEKFEKLSPEEQRKFLKALEEKKKTTPPGMN
                     EALEEENGF"
     gene            2016..3236
                     /locus_tag="VLB02_02305"
     CDS             2016..3236
                     /locus_tag="VLB02_02305"
                     /inference="COORDINATES: protein motif:HMM:NF016071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing protein"
                     /protein_id="HSE56893.1"
                     /translation="MKKIFSILLVLGAVTSCEKLPELLPVVTGEFQTKNCLYGGELTE
                     ETEREAMRTEGVVYGTKYPLTRLLVKKGKINLVLSKKPAQTTTPLVNVVLLVDFNDFG
                     DTVKNTSWNWAGPFYCPSPNLSVSEKDFILNRIKEIFARWGVLVTTDKEVFLRAPKNK
                     RVRCIVTQNMGAVFSPSGGIAFPGQMDWGDEAPCFVFVDWLFRNIKNIAEAIAHEFGH
                     AAGNEQHQSLYRADGTLQTQYNFGLIGGPINVRPVMGYGYGIGITTWWHGRSAAGYTV
                     IQNDSLLSGGRFGERPDEPSLQTLGVGETIQAVIVTADEDTYKVVVSAGKTKIQVTSG
                     GNTKLKVTVLNPAGGVVKIIDTPTGLDVPETVISNKNGTYYLRVEVSTTNLPLGTWDN
                     RPIYGGQYWITIKN"
     gene            3507..4931
                     /locus_tag="VLB02_02310"
     CDS             3507..4931
                     /locus_tag="VLB02_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HSE56894.1"
                     /translation="MSERIFIGGLPTHVGTEVRIMGWIEVRRDQGKMVFFDFRDMTGR
                     VQGVVLPNRAEVIEVAKETRTEWVVAVTGIVNKRPERNVQTGKQNGDIELEITNIAVL
                     NKAEPLPFEITTDTREVNEEVRLKYRYIDLRSERLQKNIRARDAAIQFIRNWLHDQKF
                     IEVETPILTKSTPEGARDYIVPSRLYPGNFYALPQSPQQYKQLLMAGGIERYFQIAKC
                     MRDEDTRGDRQPEFTQLDMELSFVERDDVMQLNEELLIALVTKLYPEKRIQEVPFPRI
                     SYQEAMEKYGNDRPDIREDKNDQNLLAFCWVVDFPFFESTTKSDNPDGKGEWTYTHNP
                     FSRPIEAHMLNLTEKRDIGAILTTQYDIVLNGYEIGGGSIRNHTYEALMKVFEILGHE
                     GESIERDFGHMLHAFKTGTPPHGGIAWGFDRLMMLLQNEPNIREVIAFPKTGEGRDLM
                     MESPSPVTEKQLEELSIVLKKPKQ"
     gene            5332..6108
                     /locus_tag="VLB02_02315"
     CDS             5332..6108
                     /locus_tag="VLB02_02315"
                     /inference="COORDINATES: protein motif:HMM:NF014654.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ScpA family protein"
                     /protein_id="HSE56895.1"
                     /translation="MTDQQTAFLVRHEKFEGPLEVLLELVEKRKLFVNDISLASVTDD
                     YLSYIQQKKVPAEQVAGFVAVAATLILIKARSLLPNLELSPEEAQSIEELEYRLKLYQ
                     LISAVAPKLIVLYGKKPIYLPRVKVEMVVFAPDPTLGLERLPELIKSILGRVPKEVVE
                     PKKPEAVVYKTISISEVLGSLTDRIMAAAQTTFSNIAVASPDKDEKSQKVYTIVSFLG
                     MLELVRRGFIDAEQPELFEEISIQKLEAEVVVPDLEETHE"
     gene            6101..6640
                     /locus_tag="VLB02_02320"
     CDS             6101..6640
                     /locus_tag="VLB02_02320"
                     /inference="COORDINATES: protein motif:HMM:NF016005.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HSE56896.1"
                     /translation="MSDNAKTIEALLYAEAREFSIVELAKLLQISKEAVREALEELKK
                     SLEEHGIVVVEQGEAVSLGSHPSAAKIIESLRKEMLSKELTKAAAETLAVILYHPGIS
                     KPEIEFIRGVNASYSIRLLQMRGLIEQKHKEGDARVAVYTATSEALQHFGITSAAELP
                     EFAALSEQLGTLLAHDKKI"
     gene            6637..7701
                     /locus_tag="VLB02_02325"
     CDS             6637..7701
                     /locus_tag="VLB02_02325"
                     /inference="COORDINATES: protein motif:HMM:NF013146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56897.1"
                     /translation="MILNVVKIFLPTALAFSFGILITPRLTYYMYKYKLWKKVARSEN
                     EGITNGDFKQVHNKEEELSTPRVGGVIIWLSLILCVGAIWLIGKVFPSDLSLKLDFFS
                     RSQTLIPIAALLLGAGIGLIDDILQVSGKATLYANDAINYRKIKVIAIVAIGALIGSW
                     FYFKLGITSVAIPFIGTQLALGWFFIPFFILILLAVFSTSVIDGIDGLAGGVLAVIFA
                     SYAIIAYGKDMIDIAALCGVIAGSILAFLWFNIPPARFYMGETGILGLMVVLTVIAFL
                     TDTVLLLPIIALPLAATTASVIIQIISYKYFGKRRVFKIAPLHHHFKALGWPAYKVVM
                     RYWVVSVICSIVGVVIALAG"
     gene            7704..8828
                     /locus_tag="VLB02_02330"
     CDS             7704..8828
                     /locus_tag="VLB02_02330"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative peptidoglycan glycosyltransferase FtsW"
                     /protein_id="HSE56898.1"
                     /translation="MAKGEVKPFDRYFFSVVLIITIIGIFTFVSASLGILVRSEEKFY
                     SVLFNQIILGLVCGVVGMFVVSRIHYTFWRKYAFYIFLGVLGLTLLVFVPGVGFAHGG
                     ARRWIDIGPVSFQPAELLKLGFVIYFAAWLAWVRKRGAEVKYKIAPFLVLVGIVGVTL
                     LKQPDTKSIILIIGASAAMLFVSGVSWKKIALVFGLMLVGVGILAYTRPYVMSRVQTF
                     LNPASDPQGASYQLQQSLIAIGSGGIFGRGLGQSVQKFNYLPEPQGDSIFAVYAEEFG
                     LAGTLSLLVLYIFLALRGYKIALRAPDAFAQYLVVGLVSLLTLQSFLNIGALVGILPL
                     TGVPLVFISHGGTALAIALMSAGMVLNVSRFKKEYTKKPL"
     gene            8825..9952
                     /gene="murG"
                     /locus_tag="VLB02_02335"
     CDS             8825..9952
                     /gene="murG"
                     /locus_tag="VLB02_02335"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HSE56899.1"
                     /translation="MKVLLAGGGTGGHVYPLIAIAEELNAIADEEKILNLKLYYMADA
                     PMDKDVLEENFITYIPITAGKIRVYASFKNITDLFKTGLGILGALWKVFNLYPDIVIS
                     KGGYASFPVLFAARLFRIPVIIHESDTVPGRVSRWSGKFAKRIALSFPEAAEYFPKDN
                     ISYTGQPIRKEVRSPGKEGGSSFFGLTEELPTIGIIGGSQGAQAINEAVLEILPQLVA
                     RCQIIHQVGTKNVAEVKTRADIILKDNQYKSRHKIYASLNNLQMKMFAAAVTLVVSRS
                     GSSLFEFAAWGKPSILIPYPLAHGDHQKKNAYHYARSGACAVIEEVNLTPSVLLHEIE
                     ELLTNREMYDIMAKKAKEFFKPDAARVIAAEAVSIALRHER"
     gene            9992..11332
                     /gene="gltX"
                     /locus_tag="VLB02_02340"
     CDS             9992..11332
                     /gene="gltX"
                     /locus_tag="VLB02_02340"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HSE56900.1"
                     /translation="MEHSQKVVTRFAPSPTGNLHSGAYRTAIFAYLFARKHSGTFVLR
                     IEDTDRERSKKEYEENILESLQWLGLEYDHFFRQSENIARHTQVLEKLISDGHAYVSK
                     EAAKDGSGVIKEIVRFKNPNTDVSFHDQIKGLVTMHTEDLGDFVIAKNIHEPLFHLAV
                     VVDDFDEGVTHVIRGEDHVSNTPRQVLLQRAIGAPTPEYAHLPLVLGPDKQKLSKRKG
                     ALAITEYRDLGYLPEAILNMVAMVGWNPGTDQELFSKEELITAFDLQKVQKSPAIFNE
                     EKLNWFNREYIKKLSSDSFLNYIEPYLPAYAKEDKEMLLKIEQLLRDRIEKFSDIKTL
                     FETGEFEYFFKEPTVDEAMLVWKGLKGTEDGVEKTKMYLQEIASLFEKVSAANWDYES
                     LKTALMPYAEQEGKGNILWPLRVALSGREKSPDPFELTAILGKEKTLSRITSAV"
     gene            11454..13139
                     /locus_tag="VLB02_02345"
     CDS             11454..13139
                     /locus_tag="VLB02_02345"
                     /inference="COORDINATES: protein motif:HMM:NF024991.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HSE56901.1"
                     /translation="MTQAQALTILKTGASVFLTGEPGAGKTHTINEYVRYLREHDIEP
                     AITASTGIAATHIHGQTIHSWSGIGVRRNLSSYDLDTITTTEYLARRIQKTKVLIIDE
                     ISMIDAQTLDMVEAVCRAIKQLQEPFGGIQVILVGDFFQLPPVGNQSQFAFESKAWQV
                     LCPLVCYITEQHRQSDDRFLSILSAIRKNEYGEAHHEYLQERFVANQDSPSGGETLTR
                     LFSHNADVDTLNTKELEKIKSPARTFTMKSKGKDSLVASLKKGCLSPEKLDLKIGATV
                     MCTKNSREKGFVNGTLGTVIDFEPATTYPIITTRHGDTITMEPMDWNIEENGSIKASI
                     TQVPLRLAWAITIHKSQGMSLDAAVMDLREVFEYGQGYVALSRVRSLSGLYLLGTNPR
                     VFQVHPSILAIDDRFRTESEHADVLFGDMAKTAITDMHRNFITAVGGNLTPSKKKVLK
                     QKDTRDTAEITRELVAKGKPLIIIASERSLSPTTILGHIEKLVEAGKLTFKDIEHLFD
                     KTLLRTLPEFEATFKKLDTTLLSPVFNHYNGKHSFDKLRLARLAFLARKVMLE"
     gene            13145..>13741
                     /locus_tag="VLB02_02350"
     CDS             13145..>13741
                     /locus_tag="VLB02_02350"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012414856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HSE56902.1"
                     /translation="MTPLVADKKILASVEFKPYFQKDNFVLYHGDSINLLNQLPVNSI
                     DMIFADPPYNLSNGGFTVHAGKMVSVHKGDWDKSRGFAEDYDFHYRWLEACRRVLKPE
                     GTLWVSGTYHSIYQCGHALQSLGYHILNDISWFKPNASPNLSCRFFTASHETIIWARK
                     DKKAKHMFNYELMKEGEWPEDKLKKPGLQMRSVWSMGTP"
BASE COUNT         4002 a         2961 c         3112 g         3666 t
ORIGIN      
        1 atggctgcga tcacagatag ggaacgtaga gcaaaaaatc gacctttttg ataggagtat
       61 ccgggataat attttatttg gacttccagg agatagccag atttcagatg agaagctgca
      121 gaaggttatt tctgatgcgt cacttgatga ttttattgca aaacttaaag atcacggtct
      181 tgataccatg attggagaaa gcggaattaa agtttcaggt ggcgagcgtc agcgcattgg
      241 tattgcccgg gcacttatta aagatccaaa aatccttatt tttgatgaag cgacaagtgc
      301 acttgattca atcaatgaaa aactcattca cgatgcgatt aaccggagcg caaaggggcg
      361 aacgaccatt atcatcgcac atcggctctc aaccgttatg gatgcagata ttatttttgt
      421 agttgccgat ggcaaaattg ttgatagtgg tacccataaa atacttgaaa aacgctgcaa
      481 agaatataaa aatcttatca agaaccagat actggttgcc taagttgact aattaagatt
      541 tttgtgttaa tttagtaaaa atttcataac attaaaaact taattatgaa tggaaaaatt
      601 attaaaagcc ctcaacaaac ttgtatttct tacgcctcgt acggaaactg atatacagac
      661 catacaaaaa ctagcgattg aaattcagaa agattttgta ttttcaacat actggctcgg
      721 tttgatagat aattacaccg aacagtttaa aaaaccgaac ttcaaaagtg ctatccgcag
      781 ggctatgtgg gcagcaacgc attacgtgcg tccaggaagt ggctttactc caaaactctc
      841 aacgatagag gatgataaaa gcttgggata taattgcctc ggtcgagcca ttatgcttgg
      901 gagcaatcta agatactgta aatataaatt taaagtattt ttaggaatta cccccgatca
      961 tgcaatggtg cttattgaac ttaaaaatac actataccta tgtgattcac ttgcaatgaa
     1021 gcttagaaaa ctgcatggaa catgtaatac tcacgaaggg tatcggtggt atctagggtc
     1081 gcctgaagat gaattcatga ataactattt agtcattcat gaatttgata gaggtattgt
     1141 aaatgctatt gtagaaagct ttatcttttt acaagaacct cctcaaaacc cctctggtgc
     1201 cgagcaagag tttttggtat tcaaagatcc aagattcaag ctgcatatgg caatcaatca
     1261 gctgtgctgg agagatatcc aaaaaaagta tttctcagat ttgaatgagt atcgaaatag
     1321 gtatagaaaa gagtatctgt tagagtgcgt attcatcgaa aaacagcgtc atgtacaaaa
     1381 tctccgtgct cagttcaatc aggtaactcg aagtgcgtat gaaaaagcat ttaaaagtcc
     1441 gtatgataat gagaaacata aatcttttat tgtagaaagt acttttatct tacagtctat
     1501 ccatgaacat gttatccgtt ttcttgaagg taacgtaagt gctctagatc ttcctgtgca
     1561 attaggaata tacttacaaa ctatcagaga aagagtaggc aataatgccg agcttcagca
     1621 atttactata gataaattca agtaccgcat gtctactcaa atttctatat aatctaataa
     1681 aaaacaatca gtatgaaagc agaacagtta ttagaagatg gcaagtctat tatggaaaag
     1741 tttgaaaaac tttcaccaga agaacaacga aaattcttga aagcattgga agaaaagaaa
     1801 aaaacaactc ctcctggtat gaatgaagcg ttagaggaag aaaatggttt ttaacccatt
     1861 ggtaatttaa aagcccggac tagattccgg gctttttcta tgctcttttg acaaaaagta
     1921 ttgactgttt tataatgcgt ctattctaga tataaacccc tattctggac tcttaattct
     1981 agacactgag acagttcttt aaaaacaact aaataatgaa aaaaatattt tccattctcc
     2041 ttgtactagg agcggtaact tcctgtgaga agttacctga gctgctgcca gtagttactg
     2101 gtgaattcca aaccaaaaac tgcctttatg gaggcgaatt gactgaagag actgagcggg
     2161 aagcaatgcg tactgaagga gtggtttacg gtactaaata tccgttaaca cgccttttag
     2221 ttaaaaaagg aaaaatcaac ttggtccttt ctaaaaaacc agcacaaaca actacaccgt
     2281 tggtaaatgt tgtacttttg gtcgatttta acgattttgg agatactgtt aaaaacactt
     2341 cctggaattg ggctggtccg ttttattgtc catctcccaa tctttcagta tcggaaaaag
     2401 attttattct taatagaatt aaggaaattt ttgctcgatg gggcgtgctt gtaacaactg
     2461 ataaggaagt atttttgcga gcaccaaaaa acaagcgggt gcgctgtatt gttacccaaa
     2521 acatgggcgc tgtattttcc cctagtggtg gaattgcatt tcctggtcaa atggactggg
     2581 gagatgaggc accatgcttt gttttcgttg attggttgtt cagaaatata aaaaatattg
     2641 ctgaagcaat agctcacgaa tttgggcatg ctgccggaaa cgagcagcat caatcactat
     2701 atcgtgccga tggaacactc caaactcagt ataattttgg attaattgga ggaccaatca
     2761 atgtcagacc tgttatggga tatggctacg gtatagggat taccacttgg tggcatggac
     2821 gatctgctgc tggatataca gttattcaaa atgattctct gttgtccggt ggacgatttg
     2881 gcgaacgccc cgatgagccc tctctacaaa cattgggtgt aggggagaca atacaagctg
     2941 taattgtgac tgccgacgag gatacctaca aagtagttgt ttcggctgga aaaacaaaaa
     3001 tacaggtaac aagtggtggt aataccaaac tgaaagttac cgtattaaat cccgctggcg
     3061 gtgttgtgaa aataatcgat accccaacag gtttggatgt acccgaaact gtaatttcca
     3121 ataaaaatgg aacgtattat cttcgagtag aagtgtctac gacaaattta ccattaggaa
     3181 cttgggataa tcgccctatt tatggaggtc agtattggat cactattaaa aattaaatta
     3241 gttcttttta ttttctttta agcctcgggt aaccacccgg gcttttttat tacaaacata
     3301 taaaaaatcc tgcagtatta ctgcaggatt ttaatttgtt tactatttat tatgtaaaaa
     3361 tttcaagcag ggcaagaaac gcaataataa acagatacgc aaatagaagt aagtatctct
     3421 tcggcatacc caagaagttt cctgaattat tatacaaata aatgttttgt caaaataagg
     3481 attttttggt actatgacaa acacgtatgt ctgaacggat ttttattggc ggcttgccga
     3541 cacatgttgg caccgaggtg cggatcatgg gctggattga agtgcgccgt gaccagggaa
     3601 aaatggtctt ttttgatttt cgggatatga ccggccgagt ccagggggtg gtactcccga
     3661 atcgtgccga ggtaatagaa gtagctaaag aaacccgaac cgaatgggtg gtagcagtaa
     3721 cgggtatagt aaacaagcgc ccggaacgga acgtgcagac agggaagcag aacggcgaca
     3781 ttgagctcga aattacaaat atagcagtac tgaataaggc cgagccgctc ccgtttgaaa
     3841 ttactaccga tacccgcgag gtaaacgaag aggtgcgtct aaaatatcgg tacattgatt
     3901 tgcggagcga acggctccaa aaaaatatcc gtgcacgcga tgcagccatt cagtttatcc
     3961 gcaattggct tcatgatcaa aaatttattg aagttgaaac tccgattctc accaaatcaa
     4021 cccctgaagg tgcgcgtgac tacattgtcc cgtcgcggct ctacccaggg aacttttatg
     4081 ccctaccaca atcgccgcag cagtacaagc agctgttgat ggctggcggt attgaacggt
     4141 attttcaaat cgcaaaatgt atgcgcgatg aagatacccg cggtgaccgc cagcctgaat
     4201 ttacccaact cgatatggaa ctgagttttg tagaacgcga tgacgtgatg cagttaaatg
     4261 aagaactttt gattgcactg gtgaccaaac tctatccaga aaagcgaatc caggaagtac
     4321 cctttccacg aatttcatac caggaagcga tggaaaagta cgggaatgat agacctgaca
     4381 ttcgtgaaga caaaaatgat caaaatttgc tcgcattctg ctgggtagtt gattttccct
     4441 tctttgaatc aaccacaaaa tcagataacc ctgatggaaa aggtgagtgg acctacacgc
     4501 acaacccatt ctcacgccca attgaggcac acatgctcaa tcttacggaa aagcgagata
     4561 ttggtgctat tcttaccacc cagtacgaca ttgtgcttaa tggctatgaa attggcggcg
     4621 gatcgatccg aaaccacacc tacgaagcac tgatgaaagt gtttgaaatt ttagggcatg
     4681 aaggggaatc aatcgagcgg gactttggtc acatgctcca cgcgtttaaa actggcacgc
     4741 caccgcacgg gggcattgcc tgggggtttg atcgcctgat gatgcttctg cagaacgagc
     4801 caaatatccg agaagtgatt gctttcccaa aaacaggtga ggggagagat ttgatgatgg
     4861 aatctccttc accagttact gaaaaacaac ttgaagagct cagtattgta ctcaaaaaac
     4921 caaagcaata agcttgcatt ttttacttaa tcgtggtacg ttggtggtgt aggggattag
     4981 tttcgtcttt tactactatt ccttcgggaa taataaaagt acttttaaaa gagaacagta
     5041 atcataggga gtacgcttta gcttactcac ggcactacat gccctcaaac tggcgtgttc
     5101 ataatgtata tctgacactc gtcagcgcca tcgaacatct ttttttgggt ttaatactta
     5161 tgtattttgc ccaacctcca ctctctgtga tcatttacca ttctttttgc tcttttttct
     5221 ttttgtacct tctatatatt ttaatccacc ccttcagcac agcggccttc tctacgagaa
     5281 ggccggaact tttttatggg gtaagaacgc ggtacaatac cggtaattac tatgaccgac
     5341 caacaaacgg cttttctcgt acgacacgaa aaatttgaag gtcccctcga ggtgctttta
     5401 gaactagtcg aaaagcgaaa gttgtttgtg aacgatattt cactcgcttc tgttacagac
     5461 gactatctga gctatattca gcaaaaaaaa gtgcccgctg aacaggttgc cgggtttgtg
     5521 gcagttgccg caacactcat tttaataaaa gctcgctcgc tcttgccaaa tcttgagctc
     5581 tcgcccgaag aagcacagtc gattgaagag ctcgagtacc ggctgaagct ctatcagctc
     5641 atcagcgctg ttgcaccaaa actcattgtt ctgtatggga aaaaacctat ttatttaccg
     5701 cgagtaaagg tagaaatggt agtgtttgcc cccgacccaa cactcgggct cgagcggttg
     5761 ccggagctca taaaaagtat tcttggtcgc gtgccaaaag aggttgttga accaaaaaaa
     5821 ccagaggcgg tggtgtataa aacaatctca atcagcgaag tacttgggtc actcaccgac
     5881 cgcatcatgg cagctgcaca aacaacattt agtaatattg cagtagcatc ccctgataaa
     5941 gacgaaaaaa gccaaaaagt gtatacgatt gtgtcgtttc ttggtatgct tgagctcgta
     6001 cgacggggtt ttattgatgc tgaacagccg gaactgtttg aggaaataag cattcaaaag
     6061 cttgaggcag aggtagtggt acccgatctt gaagaaacac atgagtgata acgctaaaac
     6121 aattgaagca ctgctctacg ctgaagcgcg cgaatttagc atcgttgaac tggcaaaact
     6181 cctgcagatt tcaaaagaag cagttcggga agcgctcgag gaactaaaaa aaagtttaga
     6241 agagcacggc atagtagtag tcgagcaagg ggaagcagta tcactcggct cgcatccgag
     6301 cgcagctaaa attattgaat cgctccgcaa agagatgctc tcaaaagagc tcaccaaggc
     6361 agcggctgaa acattggcgg ttattttgta ccacccagga atttcgaaac ctgaaattga
     6421 gtttattcgc ggagtcaatg cttcatactc aattcggctg ctgcagatgc gcggcttaat
     6481 tgagcaaaag cacaaagagg gggatgcgcg cgttgcagtc tacaccgcta ccagcgaggc
     6541 actccaacat tttggtatta cgagcgccgc cgagctgccc gaatttgctg cactttcaga
     6601 acagttgggc acactgctcg ctcacgataa aaagatatga ttctaaacgt tgttaaaatc
     6661 tttctcccta ccgcactggc attttcattc ggcattttaa ttacgccgcg actcacatac
     6721 tacatgtaca agtataaact ctggaagaag gtcgcccgga gtgaaaacga gggaattacg
     6781 aatggcgatt ttaaacaagt gcacaataaa gaagaagagc tttcaactcc gcgggtgggc
     6841 ggcgttatta tttggctttc gctcatactc tgtgttggag cgatatggct gattggaaag
     6901 gtgtttcctt cggatctttc gctgaagctc gattttttta gccgtagcca aacgctcatt
     6961 cctattgctg cactgcttct tggtgcaggt attggtctta ttgatgatat attacaggtt
     7021 tcaggcaagg caacgctcta tgcgaatgat gcaattaatt atcggaaaat aaaggttatt
     7081 gctattgttg ctattggtgc actcattggc tcgtggttct attttaagct aggtatcacg
     7141 agcgttgcta ttccgtttat tggcacgcag ctcgccttgg gatggttttt tattccattt
     7201 ttcattctca ttttactggc agtgttttca acatcggtta ttgacggtat cgatgggctt
     7261 gccgggggcg tactggcagt tatttttgcc tcgtacgcaa ttattgccta cggaaaagac
     7321 atgattgata tcgcggcgct ctgcggtgtt attgctggtt ctatcctcgc attcttgtgg
     7381 tttaacatcc cgccagcacg attttacatg ggcgaaacgg gtatcttggg gctcatggtc
     7441 gtgctcacgg ttattgcgtt tttgaccgac accgtactct tgctgcctat cattgcactc
     7501 ccgctggcag caaccactgc ctcggtaatc attcaaataa tttcatataa atactttggc
     7561 aaacgccgag tgtttaaaat tgcaccgctc caccaccact ttaaagcgct cggctggccc
     7621 gcgtacaaag tggtgatgcg ctactgggtc gtttcggtta tctgttcaat tgtgggcgtg
     7681 gttatcgcgc tcgctggcta gctatggcaa agggggaagt aaagccattt gaccgctact
     7741 tttttagcgt ggtgcttatc attaccatta ttggcatctt cacatttgtt tccgcatcgc
     7801 tcggtattct ggtgcggagt gaagaaaaat tctatagtgt gctctttaat caaattattc
     7861 tcggactcgt ctgcggggta gtagggatgt tcgtcgtatc gcgtattcat tacacgtttt
     7921 ggagaaaata cgccttctat atttttctcg gcgtgctcgg acttaccttg ttagtatttg
     7981 tgcccggggt tgggtttgca cacggcggtg cgcgacgttg gattgatatt ggaccagtat
     8041 cattccagcc ggcagaactc ttgaagctcg ggtttgttat ttactttgca gcgtggcttg
     8101 cctgggtgcg caagcgggga gcagaggtga agtacaaaat agcgcccttt ttggtacttg
     8161 tgggtattgt tggggtaacg ctgttaaaac agcctgatac caaaagcatt attcttataa
     8221 tcggcgcttc ggcggcaatg ctctttgtct cgggtgtgtc atggaaaaaa attgcgctgg
     8281 tgtttggttt gatgctcgtt ggtgtgggaa tacttgccta cacccgcccc tatgtaatga
     8341 gtcgagtaca aaccttttta aaccccgcga gcgacccaca aggggcatcg taccagctgc
     8401 agcagtcgct cattgcgatt ggttcgggcg ggatatttgg gcgcgggctc gggcagagtg
     8461 ttcaaaaatt taattacctg cccgagcccc aaggcgattc aatttttgct gtgtatgctg
     8521 aagaatttgg gttagcggga acactctcgc tcttggtcct ctatattttt ttggcacttc
     8581 gcggatataa aattgccctg cgggcacccg atgcttttgc acagtacttg gtagtcggac
     8641 tcgtgtcgct gcttaccttg caatcgtttt taaatattgg tgctctcgtg gggattttgc
     8701 cgctcaccgg cgtaccactg gtgtttatta gccacggagg aaccgcgctc gctattgcgt
     8761 tgatgtcggc gggtatggtc ttaaatgtgt cccgctttaa aaaggagtat actaagaaac
     8821 cactatgaaa gtactacttg ccgggggcgg cactggtggc catgtatacc ccctgatcgc
     8881 catcgctgaa gaattaaacg ctattgctga tgaagaaaag attttaaatc ttaaacttta
     8941 ctacatggca gatgcaccaa tggataaaga tgtgctcgaa gaaaacttta ttacctatat
     9001 tcccatcact gccggaaaaa tccgcgtata cgcttcattt aaaaatatta ccgacctctt
     9061 caaaacaggg cttggcattc tcggcgcgct ctggaaggtg tttaatctgt accccgacat
     9121 tgtcattagc aagggcgggt acgcaagctt tccggtactc tttgccgcgc gcctcttccg
     9181 catccccgtc atcatccatg aatccgacac cgtacccggg cgagtgagtc gctggtcagg
     9241 aaagtttgca aagcgaatcg cgctctcatt ccccgaggca gccgagtact ttccgaaaga
     9301 caacatttca tacaccgggc aacctatccg aaaagaagtg cgcagtcccg gcaaagaggg
     9361 cggctcatca ttctttggtc tgactgaaga actgcccacc atcggcatca ttggcggctc
     9421 acagggggca caggcaatta atgaagcagt gctcgaaatt ctgccgcagc tcgtggcgcg
     9481 ctgccaaatc attcaccaag tgggaacaaa aaacgtcgca gaggtaaaaa cccgcgctga
     9541 cataatttta aaagataacc aatacaagag tcggcataaa atatatgctt cactcaataa
     9601 tctgcaaatg aaaatgttcg ctgcagccgt aacacttgtt gtatcccgct cgggctcatc
     9661 actctttgaa tttgccgcat ggggcaaacc atcgatttta attccatacc cgctcgcaca
     9721 cggtgatcac caaaaaaaga atgcctacca ctacgcccgc tcgggtgcct gcgcagtcat
     9781 tgaagaagtg aacctcaccc cctcagtact gttgcatgaa atagaagaac ttcttaccaa
     9841 ccgcgagatg tacgacatta tggcaaaaaa ggcaaaagag ttcttcaagc ccgacgcagc
     9901 gcgggttatt gctgctgaag cagtctcgat tgcactgcgg cacgagcgct aacgcgttgt
     9961 ggataaagca cttttttgct acgattgccc tatggaacac tcccaaaagg tggtaacacg
    10021 ttttgcaccg agcccaaccg gcaatttaca ctcaggtgca tatcgcaccg ctatttttgc
    10081 gtacctgttt gcgcgcaagc atagcggcac ttttgtactt cgtatcgaag acaccgaccg
    10141 tgaacgttca aaaaaagaat atgaggaaaa tattcttgaa agcttacaat ggctcgggct
    10201 tgaatatgat catttttttc gccagtctga aaatatcgct cgccataccc aagtgttgga
    10261 aaaattaatt tccgatgggc atgcctacgt ttcaaaagaa gcagcaaaag atggttcagg
    10321 ggtgataaaa gaaattgtcc gatttaaaaa tccgaatacc gatgtttcat ttcatgacca
    10381 gattaaaggt ttggtaacaa tgcacaccga agacttggga gattttgtga ttgcaaaaaa
    10441 tatccacgag ccgctcttcc acttggcagt agtggtagac gattttgatg aaggggtgac
    10501 ccacgttatc cgtggtgaag atcatgtatc aaacactccg cgccaggtgc tcttgcagcg
    10561 tgctattggt gctccaacac ccgagtacgc gcacttgccg cttgtgcttg gtcctgataa
    10621 acaaaagctc tcaaaacgga aaggggcgct cgctattacc gagtaccgcg atctcggcta
    10681 ccttcctgag gcaatcttaa acatggtagc catggtgggc tggaatccgg gaactgacca
    10741 ggagcttttc tcaaaagaag aactcattac cgcctttgat ttacaaaaag tccaaaaatc
    10801 tcccgcaatc tttaatgaag aaaaactaaa ctggtttaac cgcgagtata taaaaaaact
    10861 ctcaagtgac tctttcttaa actacatcga gccgtatcta cctgcatacg caaaagaaga
    10921 taaagagatg cttctaaaaa ttgagcagct gcttcgtgat cgaatagaaa agttttcaga
    10981 tataaaaaca ttgtttgaga caggagagtt tgagtatttc ttcaaagaac caactgttga
    11041 tgaagcaatg ctcgtttgga aaggattgaa gggtactgag gatggagtgg aaaaaacaaa
    11101 aatgtaccta caagaaattg ccagcctatt tgaaaaagtt tctgcggcaa actgggatta
    11161 cgagagcctc aaaacagcgc ttatgccgta cgcagaacaa gaagggaagg gaaacatact
    11221 ctggccgctc cgcgtcgcac tctctggacg tgaaaagtca cctgatccat tcgagcttac
    11281 agcgatttta gggaaagaaa aaactctctc ccggattact tcggcggtat aaacaaaaaa
    11341 gaggcgagat agaaatctca ccccggtggc ttcattatat aaataaagcc ctcaaagcaa
    11401 gtgcatagta atgcaaggtg ctacgcacgt caactgtagt aaaataaaac ctcatgaccc
    11461 aagcacaggc acttactatt ctaaaaacag gcgcgagtgt ttttttaacc ggcgagccgg
    11521 gggcgggcaa aacacacacc attaacgaat atgttcgata cttgcgcgag cacgatattg
    11581 agccagctat cactgcctca actggcattg cagcaaccca tattcatggg caaaccatcc
    11641 attcctggag cggcattggt gtacggcgga acctctcgag ttacgacctg gataccatta
    11701 ctaccaccga atacttggcg cggcgcatac aaaaaaccaa ggtactcata attgatgaaa
    11761 tctcgatgat cgacgcccaa acgctcgaca tggtagaagc cgtgtgtcgg gcaataaaac
    11821 agctccagga gccgtttggg ggcattcagg tgattttggt gggggacttc ttccaactgc
    11881 cgccagtggg gaatcagagc caatttgcgt ttgagtcaaa ggcgtggcag gtactctgcc
    11941 cgctcgtctg ctatataacc gagcagcacc gccagagcga tgatcgattt ctctcgatcc
    12001 tttctgctat ccgtaaaaat gagtatggag aagcacatca cgaatacctc caagagcgct
    12061 ttgtggcaaa ccaagattca ccgagtggcg gagaaactct caccaggctt ttttcacata
    12121 atgctgacgt cgatacctta aacacaaaag aactcgaaaa aattaaaagc cctgcgcgta
    12181 ccttcaccat gaagtcaaaa gggaaggatt cgctcgtagc gagccttaaa aaaggctgcc
    12241 tctcacccga gaagctcgat ttaaaaattg gcgctactgt gatgtgcacc aaaaacagcc
    12301 gcgagaaggg gtttgtaaac ggcacccttg gcacggttat tgattttgag ccggcaacta
    12361 cgtacccgat cattaccacc cggcatggcg ataccattac catggagccg atggattgga
    12421 acattgaaga gaacgggagt atcaaggcga gcatcaccca ggtgccgctc cgacttgcct
    12481 gggccattac catccacaag agccagggga tgtcgctcga tgctgcagtt atggatttac
    12541 gagaagtatt cgagtacggc caagggtatg tagcgctctc gcgggttcga tcactttcgg
    12601 ggctgtacct gctcgggaca aaccctcgtg tgtttcaggt gcaccctagc attcttgcca
    12661 tcgatgatcg ttttagaaca gaatcagagc atgccgatgt actctttggc gacatggcaa
    12721 aaactgctat taccgacatg caccgcaact ttattactgc tgtcggtggc aatttaactc
    12781 catcaaagaa aaaagtgctc aagcaaaaag atacacgcga cacagccgag ataacccgcg
    12841 agctggtagc gaaagggaag ccactcatca ttatcgccag tgagcgctcg ctctcgccaa
    12901 ccaccatttt ggggcatatt gaaaaactgg tagaagcagg caaactcacc tttaaagaca
    12961 tcgaacacct cttcgacaaa acccttttgc gcaccttgcc cgagtttgaa gcaaccttca
    13021 aaaagctcga caccacactc ctttctcctg ttttcaatca ctacaacggc aagcactcct
    13081 tcgacaaact ccgcctcgcc cggttagcgt ttttggcgag aaaagttatg ttagaatagc
    13141 ctttatgact cctctcgtag cagataagaa aatactcgcg tctgtagaat ttaaacctta
    13201 ttttcaaaag gataattttg ttttgtacca tggagattct ataaatcttc ttaatcaatt
    13261 accagtaaac tcgatagata tgatatttgc tgatccaccg tacaatcttt caaatggtgg
    13321 ttttacggtg catgccggaa aaatggtaag tgtacataaa ggtgactggg ataagagtag
    13381 aggatttgca gaagattacg atttccacta ccgatggctt gaagcatgta gacgagtact
    13441 aaaaccagag ggaacgcttt gggtatcggg tacctaccac tcaatttatc agtgtggaca
    13501 tgcactgcaa tcacttgggt atcatatttt aaatgacatc tcatggttta aaccaaacgc
    13561 ttcaccaaat ctaagctgcc gtttttttac cgcaagccac gaaacaatta tttgggcacg
    13621 taaagataaa aaagcaaaac acatgtttaa ttatgagcta atgaaagagg gagaatggcc
    13681 agaagataaa ttaaaaaaac caggcttgca gatgcggtcg gtgtggtcta tgggtacacc
    13741 a
//
LOCUS       DATIBQ010000071         3322 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U16294
            k141_2243060, whole genome shotgun sequence.
ACCESSION   DATIBQ010000071 DATIBQ010000000
VERSION     DATIBQ010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935789
            Sequence Read Archive: SRR25158331
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3322)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3322)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:58:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 637
            CDSs (total)                      :: 603
            Genes (coding)                    :: 601
            CDSs (with protein)               :: 601
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3322
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2243060"
                     /isolate="SMAG_U16294"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..420
                     /locus_tag="VLB02_02355"
     CDS             <1..420
                     /locus_tag="VLB02_02355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56903.1"
                     /translation="VGLVLIIPSFISVNLRHRLASEQYAAIAESGSFASDEDLAATAA
                     AIALLKSAYAADSTFSVLAALDAIVPPPGITITNISMASREGRSIEFSGISRDRATLQ
                     QFIAAAEVSAAVASGDHPVSNFVKNQNGEFKITVRFK"
     gene            422..973
                     /locus_tag="VLB02_02360"
     CDS             422..973
                     /locus_tag="VLB02_02360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56904.1"
                     /translation="MKKEVRQQIYILTGLVLLVLGSSLLLWWAQSGVVARRTATMLLL
                     EKNASIEANRASLEIEYRDFQEVQAAVAVLNKNFYNAQTIPLFLSDVESTAQGAGIAF
                     EITSATVEANKEGGGKHVSVEFNAAGSYAALSNFSRQLKAKPYRLFFDRYYLMSQPAE
                     KSAKGARAVSWKLYGTLRLTTFK"
     gene            978..1247
                     /locus_tag="VLB02_02365"
     CDS             978..1247
                     /locus_tag="VLB02_02365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56905.1"
                     /translation="MKFTSPFSKKAKGFSRRRDRFNPHLYWRYLLGGALVLLIGAVVY
                     FALFFIQTSHILDAPVTPLASENRSKIKRMQNDIKAIQVIPSSPQ"
     gene            complement(1433..1504)
                     /locus_tag="VLB02_02370"
     tRNA            complement(1433..1504)
                     /locus_tag="VLB02_02370"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1469..1471),aa:Arg,seq:tct)
     gene            1577..1649
                     /locus_tag="VLB02_02375"
     tRNA            1577..1649
                     /locus_tag="VLB02_02375"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1610..1612,aa:Val,seq:tac)
     gene            1783..2205
                     /locus_tag="VLB02_02380"
     CDS             1783..2205
                     /locus_tag="VLB02_02380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56906.1"
                     /translation="MKKWYFASRMRHQEKIKEVCDFLTAQGEQVVCRWVYEQSLAPYK
                     QNQEKIATLAVDSIQGVIEADVFVLISDPEGTDMFVELGAALAAAVPAEKKIYNVGSY
                     SKRSLLQTHPSIIHAASLPEVFKAEQINYSKYIIPSFE"
     gene            2208..2936
                     /locus_tag="VLB02_02385"
     CDS             2208..2936
                     /locus_tag="VLB02_02385"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HSE56907.1"
                     /translation="MEILFEDNDVLLINKPAGLMVHSDGRNTEPTLVDWILEQYPSIA
                     GIGEPLRRGDTAIDRPGIVHRLDRETSGALLLAKNQDSFLFFKSQFQNRTIQKKYHAF
                     VWGHINNSPVTIDVPIGRNKGDFRKWHAGRGVRGETREAITILQTLHNFVDEQGEQFS
                     FIEAHPKTGRTHQIRVHLKYIQRPIVCDSLYNESKPAALGFTRLALHAYQITFTLSSG
                     EQKTVTAPYEPDFKATIAKYVPAC"
     gene            2950..>3322
                     /gene="rplS"
                     /locus_tag="VLB02_02390"
     CDS             2950..>3322
                     /gene="rplS"
                     /locus_tag="VLB02_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HSE56908.1"
                     /translation="MAVSITPTNKAVREKLDFASGDTVRVWSKIQEKGKTRLQAFEGL
                     VLARKHGTSQSATFTVRKVSNTIGVERIFPLYSPAIDKIEVVKKAKTRRSKLYYIREK
                     AAKVIRRKMKAVVRGKAVSEDV"
BASE COUNT          907 a          776 c          775 g          864 t
ORIGIN      
        1 gtggggcttg ttttgattat cccgtcattt atctcagtga atcttcggca tcggcttgcg
       61 agcgagcagt atgcagccat tgccgagagc ggatcatttg ccagcgatga agatcttgct
      121 gcaactgccg ctgcgattgc gctcttaaag agtgcctacg ctgctgacag caccttttcg
      181 gtacttgcag cgcttgatgc catagtgccg ccgcccggca ttacaattac caacatttct
      241 atggcttcac gcgaggggcg gagtattgaa tttagcggta tttctcgcga ccgcgcgacc
      301 ctgcagcagt ttattgctgc agccgaggta tccgcagcag ttgcttcagg tgatcatccc
      361 gtgtcaaatt ttgtgaaaaa tcagaatggt gaatttaaaa taacggttcg ctttaagtag
      421 tatgaagaaa gaagtacgcc aacaaatata tatactcacg gggttagtac tactcgtact
      481 cggcagctcg ctccttctct ggtgggcgca gtcgggcgtt gtggcgcgcc gcactgctac
      541 gatgctcctt ctcgaaaaaa atgcttcaat tgaggcaaac cgtgcttctc ttgaaattga
      601 ataccgtgat tttcaggaag tacaggcagc agtggcagtg ctcaataaaa atttttacaa
      661 tgcgcagact atcccgctct tcctctccga tgtagagtca accgcacaag gtgcaggtat
      721 tgcgtttgag attacctcgg ctaccgtaga ggcaaacaaa gaagggggtg gaaagcatgt
      781 gtcggtcgaa tttaatgcag ctggttccta cgcagcgctc agtaacttct cgcggcagct
      841 caaggcaaag ccgtaccgac tcttctttga ccgttactac cttatgagcc agcctgcaga
      901 aaaatcagca aaaggtgcgc gggcagtttc gtggaagctc tacggtacgc tccggcttac
      961 cacttttaag taatactatg aaattcactt caccattttc aaaaaaagca aaaggattct
     1021 caagacggcg cgatcgattt aatccccatt tatactggcg ctatttactg ggaggagcac
     1081 tggtgctgct tattggtgcg gtagtatact ttgctctctt tttcattcaa acaagccaca
     1141 tactcgatgc accggttact ccgctggcaa gcgagaaccg ctcaaaaata aagcggatgc
     1201 aaaatgatat taaagcaatt caggtaatac cttcgtctcc tcaataagga gccttgctca
     1261 atggtgcccc cgcgaggacg cttcgctatc tcactcggaa ctaacgttcc tctttgagaa
     1321 cccacggttc tcaacgggag aaacgccctg ctctcctcca cggaaaactt ccgttttccg
     1381 accctttccc tgttcgattc ctcgcaagaa acacacctcg ccttgcttgt tgtgcccccg
     1441 cgaggaatcg aacctcgaat aacggcttag aagtccgcag ttatatccat ttaactacag
     1501 gggcaagcgc cctgctatgc tacctttttt gatttgcaaa atcaaagata attcagtatg
     1561 ctaccctcgt tcatttgcgc tcttagctca gttggtagag cagcgctttt acacagcgaa
     1621 ggtcgggggt tcgaatccct cagggcgcac aaaacagaaa attccctaat tggggatttt
     1681 ctgttttgtg ctccaggtaa atcctcataa aaatatgata cagtatcgcc atggagcaga
     1741 aaatgatttt tttcaatttt aaaacctctg caaatttcta gtatgaaaaa gtggtatttt
     1801 gcatcgcgca tgcggcatca ggagaaaata aaagaggttt gcgattttct tacggcgcag
     1861 ggggagcagg tcgtttgccg gtgggtctac gaacagtctc ttgctcctta taaacagaat
     1921 caagaaaaaa ttgctaccct tgcagttgat tcaatacagg gagttattga agcagatgtg
     1981 tttgtactaa tcagtgaccc agaagggact gatatgtttg ttgagcttgg ggcagcactt
     2041 gctgcagcgg tacctgcaga aaagaagatt tataatgttg gttcctactc gaaacgatca
     2101 ctacttcaaa cacatccgag cattattcat gctgcatcac ttccagaagt ttttaaggca
     2161 gaacagatta attacagcaa atacataatc ccttcgtttg agtaagtatg gaaattctct
     2221 ttgaagacaa cgacgtgctc ctcatcaata aacctgctgg gctcatggtc cattctgatg
     2281 gcagaaatac cgaaccaaca ctggttgatt ggattttgga gcagtatcca tccatagcag
     2341 gaatagggga gccactacga cgcggtgata cagctattga tcgaccaggg attgtgcatc
     2401 ggctcgatag agaaacatcg ggagcgctgt tgcttgcaaa aaatcaggat tcatttcttt
     2461 tttttaaatc acaattccaa aatcgcacta tccaaaaaaa gtatcatgcc tttgtgtggg
     2521 ggcatatcaa taattctccg gttacaattg atgttcctat cggacgcaac aagggcgact
     2581 ttcgcaagtg gcatgcgggc agaggtgttc gcggagaaac ccgtgaagcg ataactatac
     2641 tacagacact acacaatttt gtagacgaac agggcgaaca attttctttt atagaggcac
     2701 acccaaaaac ggggcgaaca catcaaattc gggtgcattt aaaatatatt cagcgaccaa
     2761 ttgtctgcga tagtctgtat aacgagagca agccagcagc gcttggcttt acccgcctcg
     2821 cacttcatgc gtatcaaata acctttacgc tttcttcggg agagcaaaaa accgttactg
     2881 cgccatacga gcccgacttt aaagcaacta ttgcgaagta cgtacctgcg tgctaaagta
     2941 gtgccctata tggcagtttc aattactcca accaataaag cagtacgcga aaagctcgac
     3001 tttgcttcgg gcgatacggt acgtgtatgg tcaaaaatcc aagaaaaagg aaagactcgc
     3061 cttcaggcat ttgaggggtt agtgctcgct cgcaagcatg gcacgagtca gtcggcgacg
     3121 tttaccgttc gtaaggtatc aaatactatt ggggttgagc gtatttttcc actttactcg
     3181 cccgcaattg ataaaatcga agtggtaaag aaggcaaaga ctcgccgctc aaaactctac
     3241 tacattcgtg aaaaggcagc gaaggtgatc cgccgcaaga tgaaggcggt agtccggggg
     3301 aaggcagttt cagaagatgt ag
//
LOCUS       DATIBQ010000072         4698 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U16294
            k141_1676482, whole genome shotgun sequence.
ACCESSION   DATIBQ010000072 DATIBQ010000000
VERSION     DATIBQ010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935789
            Sequence Read Archive: SRR25158331
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 4698)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4698)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:58:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 637
            CDSs (total)                      :: 603
            Genes (coding)                    :: 601
            CDSs (with protein)               :: 601
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4698
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1676482"
                     /isolate="SMAG_U16294"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1283
                     /locus_tag="VLB02_02395"
     CDS             <1..1283
                     /locus_tag="VLB02_02395"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSE56909.1"
                     /translation="VFWNTPFEGGYSTISTFLVTNGSLVTLALIITIALVLVHGFRLF
                     NYQYPDRFTRFIAVSSLIILVPFIALLFVGLSSIVLISYGFLLIGLLIGVSVLVGRTR
                     LASFQYLKDPRSSFFIILLLVLASLSTLSSAYLATSKFVGTVSYNHALVSKDADTAIR
                     RTLRALTFSRNDLYFRTVSALYVSKFSEGAKAEKPDQALLQQYFSAAEGSAQQAIKYN
                     PADALNWQTLASVYQLIVPAKTEGAYDNARSALDRAALLSPSQPAYQLSLANLEVLHD
                     NGAGAQTAIDRAIELKPDYLDAYLLRAQLKQRAGDTDAARDALRSYTTAFPGSDAGFY
                     ALGSFELALKNYQSAREALARARALNPYNLRAQLEYVNTLKLLGQKEAAVTVLEELKQ
                     RFPNLQGVEAEIDRLKTGQATPITDTKPEVKEEE"
     gene            1335..2990
                     /gene="murJ"
                     /locus_tag="VLB02_02400"
     CDS             1335..2990
                     /gene="murJ"
                     /locus_tag="VLB02_02400"
                     /inference="COORDINATES: protein motif:HMM:NF015013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015648 - lipid-linked peptidoglycan
                     transporter activity [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HSE56910.1"
                     /translation="MNQGALLLALFSICSQVLGLVRDRALAHYFGPSETLDVYYAAFR
                     IPDFLYNSFASLVAVTALLPLLAGYLKSHRGDDARAHFRDFSHSLFTVFITAMVVVCG
                     GAFLLMKPLSFVVAPGFTEAQRELFMTLSRILLLSPIFLGLSNLFGSFSQAQKKFYAF
                     ALAPVFYNACILVGIFLIRPTVGITGVVIGAILGTVVHMSLQLPTLFRLGIYPALTLR
                     PDFSFILKVCRTSISRTVSLSLSSITILVMGALASLLAAGSVSIFNFSYNIQTTPFYI
                     VGISYAVAAFPILSRFYAEEKRKEFLEVIYKAAKNIFFFSIPLSLLFIVLRAQIVRVL
                     LGSGQFSWNDTRLVAASLALFSLSIAAQSMVLLLSRSFYAMGDTKTPLRLNSVSVLIT
                     GSVAALLLWLYNSSLSFQYFAESLLRLDGIVGSAVIVLPLSFSIGQIINACLLWGVFT
                     KRWKKLGTGVEDLRRMLLHICAAGILGAGVAYLALQVLSYATRPDTLLGIFIQGLLAG
                     ILGAAAYLGVIRLLDHNELKNMVIVITSKFWKQRPVAPEQPKL"
     gene            complement(3052..3657)
                     /locus_tag="VLB02_02405"
     CDS             complement(3052..3657)
                     /locus_tag="VLB02_02405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56911.1"
                     /translation="MDIILNELAPLPEKLEEVPPACARYIELAEQKNKANIPHEKGLI
                     LDKLIEYLSTRFAEEIKFDPKESFYKGLCQQYGNRIGRTVTSSKRKKHGVIGCLMSLQ
                     KCFEEIIESKKQKTTKGIYRQLKVTSDLKVSTTCSWYYIPESSPAYCWDHDQLIEVIL
                     SGDCYAISIELDKKKEMTEFEYNQKPNLHEALGARYSTRAF"
     gene            3812..>4698
                     /locus_tag="VLB02_02410"
     CDS             3812..>4698
                     /locus_tag="VLB02_02410"
                     /inference="COORDINATES: protein motif:HMM:NF012239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HSE56912.1"
                     /translation="MDSAHIRNFSIIAHIDHGKSTLADRLLEATGTIEARKMRDQVLD
                     SMELERERGITIKMQPVRMKYSYQGVEYVLNLIDTPGHIDFSYEVSRALKAVEGSLLL
                     VDATQGVQAQTLTTLAMAQESGLVIIPVVTKIDSPLARAEETILELAELLHVDTGSVL
                     LVSGRTGDGVQELLRAIVERIPAPVAADTNSSPQGLIFDFSYSNHKGVIVFTRLFQGS
                     IKKGDELMFSATKQKFGVLEVGVCMPHETQTESLAAGTIGYVVTGIKQPGIAAVGDTI
                     TSFQKPAPALPGYQQPRPVV"
BASE COUNT         1097 a         1142 c         1086 g         1373 t
ORIGIN      
        1 ctgtattttg gaatacccca tttgaaggcg ggtacagtac tatttctacg tttctggtaa
       61 ccaacggtag tttggtaaca ctcgcgctta ttattaccat tgctcttgta ttagtgcacg
      121 gattccgtct gtttaattac caataccctg accgcttcac tcgttttatt gcggtaagct
      181 cgctcatcat tttggtgccg ttcatcgcgc tcctctttgt cgggctctca agtatcgtgc
      241 tcatttccta cggatttctc cttattggat tacttattgg agtgtcagtg ctcgttggtc
      301 ggacacggct cgcttcattt cagtatttaa aagatcctcg ctcaagcttc tttatcatcc
      361 tgctcttggt gcttgcttct ctgagtactc tttcaagtgc gtacttggcg acctctaaat
      421 ttgtgggcac tgtttcgtat aatcatgcgc ttgtatctaa agatgcagat actgcaattc
      481 gccgtaccct tcgagcgctc acgttctcac ggaacgattt gtacttccgc actgtttctg
      541 cattgtatgt aagtaagttt tcagaagggg caaaggcaga gaagcccgat caggcactct
      601 tgcagcaata tttttcagct gcagagggga gtgctcagca ggcaattaag tacaacccag
      661 ccgatgcact caactggcag acacttgctt cagtgtatca actaattgtt ccagcaaaaa
      721 cagagggtgc gtatgacaac gcacgatccg cacttgatcg tgcagcactc ctctcgccat
      781 cgcagccagc gtaccagctt tctttagcaa atctagaggt actgcacgat aatggcgctg
      841 gagcgcaaac cgcaattgat cgtgccattg aacttaaacc tgattacctt gacgcatatc
      901 tcctccgtgc tcagctcaag cagcgagcag gagataccga tgctgcacga gacgcgctcc
      961 gttcatacac tactgctttt ccgggatcag acgctggatt ctacgcgctc ggctcgtttg
     1021 agctagcgct taaaaactat caatcagccc gcgaggcgct tgctcgtgct cgtgcgctta
     1081 atccctacaa ccttcgcgcc caacttgagt acgtaaacac actgaagctc ttaggacaaa
     1141 aagaagcagc agtcacggta cttgaagaat taaagcagcg gtttccaaac ctgcaaggag
     1201 tcgaagctga aattgaccgc ctaaaaacag gtcaagctac gccgataacc gatacgaaac
     1261 cagaagtaaa agaagaggag taatacacaa tggtttccaa actcctcagt ctgctcaata
     1321 gggaagtaaa aactatgaac cagggcgcac tcttgctcgc gctcttctct atttgctcac
     1381 aggtgctggg attagtacgg gacagagcgc tcgcgcatta ctttggtccg tccgagacgc
     1441 tcgatgtgta ctacgccgca tttcgcattc ctgatttcct ctacaattca tttgcctcac
     1501 tggtggcagt aacggcgctc ttgccgctac tggctgggta tctaaaaagc catcgaggtg
     1561 atgatgcacg agcacacttt cgcgattttt ctcacagcct gtttacggtg tttataacgg
     1621 cgatggtggt agtgtgcggc ggcgcatttt tgctgatgaa gccactctcg tttgtggttg
     1681 cccccggctt tactgaagcg cagcgggagc tttttatgac gctctctcgc attcttctcc
     1741 tttcaccaat ctttctgggg ctttcaaatt tgtttggatc attttcacag gcgcaaaaaa
     1801 agttttatgc attcgcactc gctccggtgt tttacaatgc ctgtattctt gttggtatct
     1861 ttctcatccg cccaacagtt ggtattaccg gagtagtcat tggtgcaatt ttaggcacgg
     1921 tggtgcacat gtcgctgcag ctaccgacac tttttcgcct aggcatctac ccagccctta
     1981 ccttgcgccc ggatttttca ttcatcctaa aagtatgtag gacttcaatc tcacgcactg
     2041 tgagcctctc gctctcgagc attactattt tagtaatggg cgcactcgct tcgctcttgg
     2101 ctgcggggtc agtttcaatt tttaattttt cgtacaacat ccaaacaacc ccgttttata
     2161 tcgttggcat ttcttatgca gtagcggcat ttcctatcct ttcacggttt tatgctgaag
     2221 agaagcgcaa agagttttta gaagtcatct acaaagcggc aaaaaatatt ttcttttttt
     2281 caattccgct ctcacttctc tttattgtgc tccgggcaca gattgtgcgc gttttattag
     2341 ggtcggggca gttcagttgg aacgatacgc gcctggttgc tgcatcactc gcactcttct
     2401 cgctctcgat tgcagcgcag agcatggtgc tgctcctctc gcggagcttc tatgcgatgg
     2461 gggataccaa aacaccgctt cggttaaaca gtgtttcagt acttattacc ggcagcgttg
     2521 ccgcgctgtt actctggctc tacaatagtt cgctctcgtt tcaatacttt gctgaatcac
     2581 tgcttcggct cgatggcatt gttggaagcg ccgtaattgt gctgccgctc tcgttttcga
     2641 ttggtcagat tataaacgcg tgtctcttgt ggggggtatt cactaagcgc tggaagaagc
     2701 tcggcactgg agttgaagac ctccgccgaa tgctcttaca tatttgcgca gcgggcatct
     2761 taggcgcagg cgttgcttac ctggcgcttc aggtgctctc gtacgccact cgtcccgata
     2821 ctttgttggg tattttcatc caggggcttc tagcgggcat cttaggcgca gcagcctacc
     2881 tgggagtcat ccgcctgctc gaccataacg agcttaagaa catggtcata gtcatcactt
     2941 ctaaattttg gaaacagcgc cccgtggcac cagaacagcc gaagctgtag tttggtgctg
     3001 tatccaaaaa caaaaaagcc gcctggtatt tgttaccagt acggcttcac tttaaaatgc
     3061 tcttgttgaa tatcttgccc ctagtgcttc gtgaagatta ggtttttggt tgtattcaaa
     3121 ttcggtcatc tcttttttct tatccagctc aatcgagata gcgtagcagt ctcccgaaag
     3181 aatcacttca ataagctggt catggtccca acaatacgct ggagaagatt cgggaatata
     3241 gtaccaactg caagtggtgc taacttttaa atcgctggta actttcagct gtcggtaaat
     3301 accttttgtc gttttttgtt ttttagattc aataatttcc tcaaagcatt tttggagact
     3361 cattaagcaa cctattaccc catgtttctt ccttttacta gaagttactg tccgcccaat
     3421 tcgatttcca tattgttggc agaggccttt gtaaaagctt tccttcgggt caaattttat
     3481 ttcttcggca aaccttgtac ttaaatactc gataagtttg tcgagaatca atcctttttc
     3541 atgaggaatg tttgctttat ttttttgttc tgcaagttca atgtatcttg cacaagcagg
     3601 tggcacctcc tctagttttt ctgggagagg ggcaagttcg ttcagtataa tatccattgc
     3661 atagttgttt taaatgaaaa aattctattg tagagtaaca catatataac aaaaagtcaa
     3721 tttttaagtt gagtacttaa tctttacttg ggtcattgag gttttttatg aatttttttg
     3781 tgtatgctat agttcctcaa cacactataa tatggattca gcacatatcc gaaatttctc
     3841 aatcattgct catatcgacc acgggaagag cacccttgcc gaccggctgt tagaagcaac
     3901 gggcaccatt gaagcgcgga aaatgcgtga ccaggtgctc gattctatgg agctcgagcg
     3961 agagcggggg attaccataa aaatgcagcc agtccggatg aagtactcgt accagggtgt
     4021 tgaatatgtt cttaacctta tcgatacgcc agggcacatc gatttttcat acgaagtttc
     4081 tcgtgcactg aaagcggttg aagggtcgct cttgcttgtt gatgcaaccc agggcgtgca
     4141 agcgcaaaca ctcaccacac ttgcgatggc gcaggaatcg gggctggtga ttatcccggt
     4201 ggttaccaaa attgattctc cgcttgcgcg cgcggaagaa acgatactcg agcttgctga
     4261 actcttgcac gttgatactg gatcagtgct gctggtttct ggcaggacgg gtgatggggt
     4321 acaagagctc ttgagagcaa ttgtagagcg gatcccagcg ccagtagctg ccgacacaaa
     4381 cagtagcccc cagggtttga tttttgattt cagctactca aatcacaaag gggtaattgt
     4441 atttacacga ttgttccagg ggagtattaa aaagggggac gagctcatgt tctcagcaac
     4501 gaagcaaaag ttcggcgtgc ttgaagtggg agtgtgcatg ccgcacgaaa cacaaaccga
     4561 aagcttggca gcgggtacca ttggctacgt agttaccggc atcaagcagc ccggcattgc
     4621 agcggttggg gacaccatta cgagctttca gaagcccgca ccagcactcc ccgggtacca
     4681 gcagccgcgg ccggtggt
//
LOCUS       DATIBQ010000073         9516 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U16294
            k141_881738, whole genome shotgun sequence.
ACCESSION   DATIBQ010000073 DATIBQ010000000
VERSION     DATIBQ010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935789
            Sequence Read Archive: SRR25158331
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 9516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:58:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 637
            CDSs (total)                      :: 603
            Genes (coding)                    :: 601
            CDSs (with protein)               :: 601
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9516
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_881738"
                     /isolate="SMAG_U16294"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..793
                     /locus_tag="VLB02_02415"
     CDS             <1..793
                     /locus_tag="VLB02_02415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56913.1"
                     /translation="TVPGFKTVEGKRVAGVSPATVVTATAAGSDYNSKGTDFAVSGYT
                     TGDKAKKFYGRSSGPLAGGVSGNMHTVSEASRVEITGVLQQLLAEKLKRQARAQLPEG
                     FITFPDFQYMLYNSASLTLQSPTIKFPAALSGTLVTYLFPREPLARAIAELAVENGSG
                     GAATGITYTVPDVADLTISQGLALAISTEEIPKELTLQVTGEGTMRAALDQNALKEAL
                     VGEPTKNFETLMAAFPGIAEARFSRFPFWTPYFPNNPEKIKLIIK"
     gene            839..1051
                     /gene="infA"
                     /locus_tag="VLB02_02420"
     CDS             839..1051
                     /gene="infA"
                     /locus_tag="VLB02_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010939491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HSE56914.1"
                     /translation="MGAQPQTTVRGTIHEALPNTLFRVTLADGAEILAYLAGKMRLNR
                     IKVLVGDTVEVVVDQYGGKGRIVKRM"
     gene            1099..1209
                     /gene="rpmJ"
                     /locus_tag="VLB02_02425"
     CDS             1099..1209
                     /gene="rpmJ"
                     /locus_tag="VLB02_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012693977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HSE56915.1"
                     /translation="MKVKSTVKKMCENCKIVKRGNHLFVLCSNPKHKQKQ"
     gene            1209..1592
                     /gene="rpsM"
                     /locus_tag="VLB02_02430"
     CDS             1209..1592
                     /gene="rpsM"
                     /locus_tag="VLB02_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010879984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HSE56916.1"
                     /translation="MMRILGITIPNEQRLEFGLTVLYGIGQSTARKILARTNVDPGKK
                     PGDLTIDEENTVRKAVEEMKIEGNLKREVSANIKRLKDVKAYRGVRHQRRLPVRGQRT
                     KTNSRTIRGNVRQTMGSGRRKESKT"
     gene            1596..2030
                     /gene="rpsK"
                     /locus_tag="VLB02_02435"
     CDS             1596..2030
                     /gene="rpsK"
                     /locus_tag="VLB02_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HSE56917.1"
                     /translation="MGKKRIATQEGQLVDADKRNRATSRAPKKKITVAQLSVEATFNN
                     TKAVVSDMKGNVLFWSSSGSLGFKGAKKGTPFAASKVGEILAEKATAAGIKEVNAVVK
                     GVGSGREPLVRAFIASGIDLLSIKDKTPVPHNGPKPKKPRRV"
     gene            2034..2651
                     /gene="rpsD"
                     /locus_tag="VLB02_02440"
     CDS             2034..2651
                     /gene="rpsD"
                     /locus_tag="VLB02_02440"
                     /inference="COORDINATES: protein motif:HMM:TIGR01017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HSE56918.1"
                     /translation="MKTRPRFKMCRRVGAPIFEQCQTQRFIQSEAKRGRATSSPNAKR
                     PKPLSDYGQQLLEKQKVRFSYGITERQLANYVKTATATKGGTVTDKLYSLLEMRLDNV
                     IYRLGLAHTRRFARQLVSHGHITVGGKRINVPSYQVRVGDVIVVREGSRGSTVFADFA
                     KRAEQHTVPNWLTLVPAQLTGTVKGTPKHDDPFMKLETVLEFYSR"
     gene            2696..3694
                     /locus_tag="VLB02_02445"
     CDS             2696..3694
                     /locus_tag="VLB02_02445"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HSE56919.1"
                     /translation="MSEQKIILPSKPKAVLEEGFKGVYEIDGLYPGYGYTLANSLRRI
                     ILSSLEGAAITGVKIDGVSHEFSTIEGVKEDVVTILLHLKNIRFRVTGTEPQKVTLSV
                     KGPKVVTAGDLKASGQVEVINADQYICELTAKTALSMELTLEKGRGFVTREQHQKEKA
                     EIGTIFLDAIFSPIRRVSYDVEDMRVGDKTNYNRIRLVIETDGTIAPREALEQSVKTM
                     IEQLAAIVNVDSNEIIQAVQSQVSSARVESKEEATAEAATDDSQADVTDILKTRIDAI
                     GLSTRTLNALSAANIRTVGGVARKHVDDLLEIEGIGEKGVQEIKKALAVYSLTLRD"
     gene            3707..4060
                     /gene="rplQ"
                     /locus_tag="VLB02_02450"
     CDS             3707..4060
                     /gene="rplQ"
                     /locus_tag="VLB02_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HSE56920.1"
                     /translation="MRHHNANRTFGRSKNQRAALMKGLVESLVTHGKIETTEAKAKEL
                     RPAIEKMVTKAKKPTLATRRLLLASLYNHTEVVEKLIGTIAPKYAERAGGYTRIIKLP
                     QRAGDASPMAVIEFV"
     gene            4083..4427
                     /gene="rplM"
                     /locus_tag="VLB02_02455"
     CDS             4083..4427
                     /gene="rplM"
                     /locus_tag="VLB02_02455"
                     /inference="COORDINATES: protein motif:HMM:TIGR01066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HSE56921.1"
                     /translation="MQYTVDATGKTLGRLASDVASKLMGKGSPTFMRNKLSGNAVTVT
                     NAAKLKVAEQKLREKTYIQYSGYPGGQKIESMQAVITKKGYGEAIRKAVKGMLPDNKL
                     KNGMMKQLTVTE"
     gene            4429..4842
                     /gene="rpsI"
                     /locus_tag="VLB02_02460"
     CDS             4429..4842
                     /gene="rpsI"
                     /locus_tag="VLB02_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HSE56922.1"
                     /translation="MERIAKTNKKYIETVGRRKTATARVRVSPATKGSYDINGKDLAT
                     YFPTAELQKTVTDTFAKLETSDSYAVTVRVSGGGIHAQAEAVRHGIARALVEENTEVK
                     PKLKQAGYLKRDPRSKERRKFGLKKARKAPQWSKR"
     gene            4918..5292
                     /locus_tag="VLB02_02465"
     CDS             4918..5292
                     /locus_tag="VLB02_02465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56923.1"
                     /translation="MTNILGALFPFGTGIICVYIVYELYIIPLRTKKILNSLSNLSDQ
                     TDGSIFIIQGMLHNNTVIALYSEREKVIRYFKYIPKDFPKDLVEVGRSFIFRVHYKDG
                     KADFYFQALFRMAVREHGGLLC"
     gene            5372..5923
                     /locus_tag="VLB02_02470"
     CDS             5372..5923
                     /locus_tag="VLB02_02470"
                     /inference="COORDINATES: protein motif:HMM:NF013212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HSE56924.1"
                     /translation="MDDERNDVEFEASDDEGGALTGVQKLKELREKLKQAEKEKQEYL
                     DGWQRAKADYVNLQKQLDEARIRAKTITEEQFFDDLLPVLDSFYFAFNNKEAWEAVDA
                     NWRTGVEYIYGQLKGILEARGLSSYDDTGAPFDPFRHQAVTEIETEDTAQAGTIAATL
                     KLGFIYKDTVLRPASVSVYTLKK"
     gene            5940..7811
                     /gene="dnaK"
                     /locus_tag="VLB02_02475"
     CDS             5940..7811
                     /gene="dnaK"
                     /locus_tag="VLB02_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HSE56925.1"
                     /translation="MAKIIGIDLGTTNSAVAIIEGGQPKIIENIEGNRTTPSIVAAAK
                     NGDRLVGLLAKRQAVTNPQNTIYGIKRFMGHNFDDAEVQRIVKTSAFNVSKGSDGGVL
                     VKLGDKEYRPEEISAMILQKIKADVEAKLGEKITEAVITVPAYFNDAQRKATKDAGAI
                     AGLDVKRIINEPTAAALAYGFNTRKNEKIAVFDFGGGTFDVSILEVTEDVIEVKATDG
                     DVFLGGRDIDHKIMYWIADEFKKESGVDVTKDPLALQRLDEAAEKAKIELSTTPETEI
                     NIPFITSDASGPRHLLLKMSRAKLEEIAADYITRAMEITKRAVEASPFKMNEIDQVIL
                     VGGQTRMPKVVEEVKKLFGKEPNRSINPDEVVALGAAVQGGVLQGDVRDVLLLDVIPL
                     SLGIETLGSVATKLIERNTTIPTSRSQVFSTAADNQTSVQIHVVQGERPMANDNKSLG
                     QFVLDGIAPAPRGMPQVEVTFDIDANGILQVKAKDKATGKEQSIRIEATSGLSKEDIE
                     RMQKDAEIHADEDQKKKELVDAKNTAEMSIFAAEKSLQDYKDKITEEIKTEVETKVAE
                     LKAIKDGSDLSALQTKTAELSTAMQKIGEHIAKQAPEQESQSSSTDTPPEGEQTNTQ"
     gene            7993..9069
                     /gene="dnaJ"
                     /locus_tag="VLB02_02480"
     CDS             7993..9069
                     /gene="dnaJ"
                     /locus_tag="VLB02_02480"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF008035.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HSE56926.1"
                     /translation="MAKDYYNILGVQKGASKDEIKKAFHKLAHQYHPDKQHGDEAKFK
                     EVNEAYQVLSDDQKRAQYDNFGSSFQQSGGAGPGGFDFNGFDFSNFQNGQGFDFDLGD
                     IFGDFFGGGKRGRARTERGRDLQVEVKLTFKEAIFGAEKPVTVKRTATCKTCSGSGAK
                     PGTNLKTCSKCNGHGTIRDMQRTIFGSVATTRTCDVCSGAGKIPEHPCATCKGTGLTK
                     DTSTLNVVIPPGVQHGEMLRLGNMGEAVRGGETGDLYLQITVEPHKTIIRDGNRLVTT
                     LDIKLTDALLGAQYAVETLDGSIPVTIPAGTKIGSIVTLKNHGVPSPQGKRGDFLIKL
                     NIKLPEKISSKAKAIIETLKEEGI"
     gene            9094..>9516
                     /locus_tag="VLB02_02485"
     CDS             9094..>9516
                     /locus_tag="VLB02_02485"
                     /inference="COORDINATES: protein motif:HMM:NF013499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HSE56927.1"
                     /translation="MSSIEEVRSERIKKLAALKEKGIDPYPAVSHRTYSIARFQSEFA
                     ALADAKQPVTIAGRIMSKRGQGGVTFCDIFDGTSKTQIFLKSDEMDAAQYALFTETVD
                     VGDFLEASGAAYLTKRGEQSLFVSNWSMLTKSLRPIPDA"
BASE COUNT         2876 a         2099 c         2338 g         2203 t
ORIGIN      
        1 aacggtgccc gggtttaaaa cggtagaagg gaagcgtgtt gcaggagtga gccctgcaac
       61 agtagttacc gcaacagccg cagggagcga ttacaatagc aagggaaccg attttgccgt
      121 ttcaggctac actacaggcg ataaggcaaa aaaattctac ggtcgttcga gtggtccgct
      181 tgccggcggg gtaagcggta atatgcacac tgtttcagaa gcaagcaggg tagaaattac
      241 cggcgtctta cagcagctct tggctgaaaa attaaagcgc caggcgcggg cacagctgcc
      301 tgaagggttt ataaccttcc ccgattttca gtacatgctc tacaacagcg catcgctcac
      361 gctccagagt ccaactatta aattcccggc ggcactctca ggtacccttg ttacctatct
      421 gttcccacgc gagccgctcg cccgtgccat tgccgaactc gctgttgaaa acggttcggg
      481 cggtgctgca acgggcatca cctacaccgt gcccgatgtt gccgacctca ccattagtca
      541 ggggctcgcg ctcgctatca gtaccgaaga aattccaaaa gagctcacct tgcaggtaac
      601 gggcgaggga acaatgcgcg ctgcactcga tcaaaacgca ctaaaagagg cgctcgtcgg
      661 cgaaccaacc aaaaattttg aaacactcat ggctgctttt cccggcattg ccgaagcccg
      721 cttctctcgc tttccgttct ggacccccta tttccccaac aatcccgaaa aaattaagct
      781 gattattaaa taaaattgac ttctgcttaa aaatttttta tagtgatagt actagcacat
      841 gggggcacaa ccacaaacta cagttcgagg caccattcac gaagctttgc cgaatacatt
      901 atttcgggta acgcttgctg atggagctga aattcttgcg taccttgcag gaaaaatgcg
      961 cttgaaccgt atcaaggttt tggttggcga taccgtcgag gtagtggtcg accagtacgg
     1021 gggaaaagga cgtattgtca aacggatgta gttggtgtac aatccccgag ctctcatttg
     1081 tgacagcttt tttattttat gaaagtcaaa tcaaccgtca aaaagatgtg cgaaaactgc
     1141 aaaattgtta agcggggaaa ccaccttttt gtgctgtgct caaaccctaa acataagcaa
     1201 aaacagtaat tatgcgaatc cttggtatca ctatccctaa cgaacaacgg cttgaatttg
     1261 gcttgactgt tctctatggt attgggcaat caactgctcg caaaatactc gctcgtacga
     1321 acgttgatcc tggtaaaaag cctggcgatc taaccattga cgaagaaaac actgttcgaa
     1381 aggcagttga agaaatgaag attgaaggaa acttgaaacg tgaagtttct gcaaacatta
     1441 aacgcttaaa agatgtaaaa gcatatcgtg gggtacgcca ccagcgccga ttgccggtgc
     1501 gcggacaacg aactaaaaca aattcacgaa ccatccgtgg aaacgttcgc caaactatgg
     1561 gtagtggtcg tcgtaaggaa tcaaaaacat aacatatggg aaagaaacgt attgcaacac
     1621 aagaagggca gttggttgat gctgataagc gcaatcgggc aacttcgcgt gctccaaaaa
     1681 agaagattac cgtggcgcag ctttctgttg aggcaacatt caataatacc aaggcagttg
     1741 tttcagatat gaagggaaat gtgctttttt ggtcttcaag tggttcactc ggttttaagg
     1801 gtgcaaaaaa gggtaccccg tttgccgcat caaaagtggg tgaaatttta gccgaaaaag
     1861 caactgctgc cggtattaaa gaggtaaacg cagtggtaaa gggggtaggc agcggtcgtg
     1921 agccgttggt acgtgccttt attgcaagcg gtatcgatct cctctcaata aaagataaaa
     1981 cgccggtgcc gcacaatggt ccgaagccta agaaacctcg acgagtataa cctatgaaaa
     2041 caagaccccg atttaaaatg tgccgacgtg ttggcgcccc aatatttgaa caatgccaga
     2101 cccagcggtt tatccagtcc gaggcgaagc gcggtcgtgc tacttcgtct cctaatgcaa
     2161 agcgtccaaa gccgctctct gattacgggc agcagctttt agaaaaacag aaggtacgat
     2221 tttcatacgg tattaccgag cgccagcttg caaactatgt aaagacggca actgcaacaa
     2281 aaggcggaac agtaaccgac aagctctaca gcctgcttga aatgcggctc gataacgtta
     2341 tttatcgcct tggtttggca cacactcgtc gctttgctcg ccagctcgtc tcacacggac
     2401 acattaccgt cggcggaaag cgaataaacg ttccttcata ccaggtgcgg gtgggggatg
     2461 tgatcgttgt tcgcgaaggc agccgcggct caacggtgtt tgccgatttt gcaaagcgtg
     2521 cagaacagca cacggttcct aactggctta ccttggtgcc ggcgcagctt accgggacgg
     2581 tgaaggggac accgaagcac gatgacccat ttatgaagct cgaaacagtg ctcgagttct
     2641 acagtcgata gtagaaattt gatttatttt aaaaatagtt gtattattta tttctatgag
     2701 tgagcaaaaa atcatcctac catcaaagcc gaaggcagta ttagaagagg ggtttaaagg
     2761 ggtttatgag atagatgggc tctacccagg ctatggctac acgcttgcca atagtctccg
     2821 ccgtattatc ctttcttcac ttgaaggcgc tgctattact ggcgttaaaa tcgatggtgt
     2881 cagtcatgaa ttttctacga ttgaaggagt taaagaagac gtggtaacca tactcctcca
     2941 cctcaaaaat atccgcttcc gggttaccgg taccgagcca caaaaagtaa ccctctccgt
     3001 aaaaggtcca aaggtggtta ctgccggaga cttgaaggcg agtggtcagg ttgaagttat
     3061 caatgccgac caatacatct gcgaactcac tgcaaaaaca gcgctctcga tggagctcac
     3121 ccttgagaag gggcgtggtt ttgtaactcg tgaacagcac caaaaagaaa aagctgaaat
     3181 cggtactatt tttcttgatg ctattttttc tcctattcgc cgtgtgagtt acgatgttga
     3241 agacatgcgt gtgggtgata aaacaaacta caatcgcatc cgcctcgtga tcgaaactga
     3301 cggcactatt gctccgcgtg aagcacttga gcagtcagta aaaacaatga tcgagcaact
     3361 tgctgcaatt gtaaacgttg atagtaacga aataatccaa gcggtacagt cgcaggtttc
     3421 ttctgctcgt gttgagagta aagaggaagc aactgctgaa gctgcaacag acgattcaca
     3481 agcagatgtt accgacattc ttaaaacacg catcgatgcg attgggcttt caactcgaac
     3541 cttaaacgcg ctctctgcag caaatatccg gaccgtaggc ggtgttgccc gcaagcatgt
     3601 tgatgacctg cttgaaattg aaggcatcgg agagaagggg gtacaggaaa taaagaaagc
     3661 attggcagtg tatagcctta cccttcgaga ttaatcgttt ttatatatga gacaccacaa
     3721 cgctaacaga acattcggac gcagtaagaa ccagcgcgca gcccttatga aagggttggt
     3781 agagtcgttg gttacccacg gcaagattga aactaccgag gcaaaagcaa aagaacttcg
     3841 ccctgcaatc gagaagatgg taacaaaggc gaaaaaacca acactcgcta ctcgccgact
     3901 cttgctcgcc tcactctaca accataccga ggtggtagaa aagcttattg gcacaattgc
     3961 accgaagtat gctgagcgtg ccggtgggta tacacggatt attaaactcc cacagcgagc
     4021 gggtgatgcc agcccaatgg cagtgattga attcgtataa atttttgata cagtacagaa
     4081 ctatgcaata cacagttgac gcaacaggaa aaacactcgg acgactcgcc agcgatgtcg
     4141 cttcaaaact catgggtaaa ggaagcccga catttatgcg caataagctc tcggggaacg
     4201 ccgtaaccgt tactaacgct gcgaagctca aagttgccga gcaaaaactt cgagaaaaaa
     4261 cctacattca atattcggga tacccgggcg gacaaaaaat agagagcatg caagccgtta
     4321 ttactaaaaa aggctacgga gaggcgatcc ggaaagcggt taaaggtatg cttcctgaca
     4381 acaagcttaa aaatggcatg atgaagcaat taacagtaac cgaatagtat ggaacgaatc
     4441 gcaaaaacaa ataaaaaata tatcgaaacc gttggtcgcc gcaaaacagc tactgctcgt
     4501 gttcgcgtca gcccggcaac aaaaggaagc tacgatatca acggaaaaga tttggcaacc
     4561 tactttccaa ctgccgaact ccaaaaaaca gttaccgata cgtttgcaaa acttgaaact
     4621 agtgattcat acgcagtaac cgttcgagta agcggtggcg gtattcatgc tcaggctgaa
     4681 gcagttcgcc acggcattgc tcgtgcactt gttgaagaaa ataccgaggt aaagccaaaa
     4741 ctcaagcagg caggatactt gaagcgtgat ccgcgcagta aagagcgtcg caagttcggt
     4801 ctcaaaaaag cccgcaaagc cccacagtgg agtaagcgtt aaaaaaaatc cccgaaaggg
     4861 gatttttcat tgacacaaaa gtatttgggt tatattgtgt taaaaaaact aattgctatg
     4921 acaaacatat taggagcact ttttcccttt ggtactggga tcatatgcgt ttatatcgta
     4981 tatgagctgt atatcattcc tttacgtact aaaaaaatcc tcaatagtct ttctaatctc
     5041 tctgatcaaa cagatgggtc tatattcata attcagggta tgttacacaa taacacggta
     5101 atagcactct attccgaaag agaaaaagtt attcgttatt ttaaatatat tcctaaagat
     5161 ttccccaaag atcttgttga agtaggaaga tcgtttattt tccgagtcca ctataaggat
     5221 gggaaagcgg atttttattt ccaagcacta tttcggatgg ctgtaagaga gcatggagga
     5281 cttctgtgtt agttttttgt acaaaattgt taaagcttca gccttgggct aggctttttt
     5341 tatttgaaaa attttgtata atcacacccg tatggatgat gaacgaaacg atgttgagtt
     5401 tgaggcaagc gacgatgagg gtggtgcgct gacaggtgtt cagaaattaa aagagctgcg
     5461 tgaaaaactg aagcaggcag aaaaggaaaa acaggagtat ctagacggct ggcagcgggc
     5521 aaaagctgat tatgtgaatc tccaaaaaca gcttgatgaa gcacggattc gggcaaagac
     5581 gattaccgag gagcagttct ttgatgacct ccttcctgtt cttgacagtt tttactttgc
     5641 cttcaataat aaggaggcat gggaagccgt agacgcaaac tggcgcacgg gggttgaata
     5701 tatttacggg cagctgaagg gaatcttgga agcgcgcggg ctctcatcat acgacgatac
     5761 aggagcaccg tttgatccat ttcggcatca ggcggtaacc gagatagaaa cagaagatac
     5821 agcacaggct ggtactattg cggcaacact gaaactcgga tttatctata aagatacggt
     5881 attgcgacca gcttctgttt cagtgtacac acttaaaaaa taatttggac ttttgaacta
     5941 tggcaaaaat aattggaatt gatcttggaa caacaaactc ggcagtcgct attattgaag
     6001 gcgggcagcc gaaaatcatc gaaaatatcg aaggaaatcg caccacccct tctattgtgg
     6061 cagcggcaaa aaatggcgat cggctagtgg ggcttttagc aaaacgccag gcagtaacga
     6121 atccgcaaaa tactatctat ggcattaagc gcttcatggg gcataatttt gatgatgctg
     6181 aagtgcagcg aatcgtaaag acctcggcat ttaatgtttc aaaaggatcc gacggcggcg
     6241 tgctcgtaaa actcggtgat aaagagtacc gccctgaaga aatctcggcg atgattctcc
     6301 aaaaaattaa ggcagatgtt gaagcaaagc tcggcgaaaa aattaccgag gcagttatta
     6361 ccgtgccagc gtactttaac gatgcacagc gtaaagctac aaaggatgca ggtgcaattg
     6421 cagggcttga tgtaaagcgc atcattaacg agccaaccgc tgcggcgctt gcgtatgggt
     6481 tcaatacacg aaagaacgag aaaatcgccg tgtttgactt tggtggcggc acctttgacg
     6541 tttctattct tgaagtaact gaggatgtga ttgaggtgaa ggcaaccgat ggcgatgtgt
     6601 tcttgggcgg tcgtgatatt gatcacaaaa tcatgtactg gattgccgat gaatttaaaa
     6661 aagagagtgg agtagatgtt accaaagatc cgctcgcact tcagcgcttg gatgaggcag
     6721 ccgagaaagc aaaaattgag ctttcaacga caccagaaac cgaaattaat atcccattta
     6781 tcacgagcga tgcatctggt cctcggcacc tgttgcttaa aatgtctcga gcaaagcttg
     6841 aagaaatcgc agctgattac ataacgcgcg cgatggaaat taccaagcgc gcggttgaag
     6901 catctccatt taaaatgaat gaaattgatc aagtaatttt ggtgggcgga caaacccgca
     6961 tgccaaaagt ggtagaagag gtgaagaagc tctttggtaa ggaaccaaac cgctcaatta
     7021 accctgatga agtagtggca cttggtgcag cggtgcaagg cggtgtgctc cagggcgatg
     7081 tgcgcgatgt gctcctgcta gacgtgatcc cactctcgct cggtattgaa acattgggga
     7141 gtgtggcaac aaaacttatt gagcgaaaca caaccatccc aacaagtcgc tcgcaggtgt
     7201 tctcgaccgc ggctgataat cagacttcag tgcagattca tgtggtgcag ggcgagcgac
     7261 caatggcaaa cgataacaag tcgctcgggc aatttgtgct cgatggtatt gcgccagcgc
     7321 cgcgcggtat gccccaggtt gaggtaacct tcgacattga tgcaaacggg atccttcagg
     7381 taaaggcaaa agataaagca accggcaaag agcagtcgat ccgcatcgag gcaacctcgg
     7441 ggctctcaaa agaagatatc gaacgcatgc agaaggatgc cgagattcat gctgatgaag
     7501 accaaaagaa gaaagagttg gtagatgcaa aaaatactgc tgagatgtct atctttgctg
     7561 ctgaaaaatc actgcaagac tataaagata aaataacgga agaaatcaaa actgaagttg
     7621 agacaaaagt tgcagagctg aaagcaataa aagacggcag cgatttaagc gcgctccaaa
     7681 ccaaaactgc agaactctca actgcaatgc aaaaaattgg cgaacatatt gcgaaacagg
     7741 caccagaaca ggaaagtcaa tcaagctcaa ctgatacccc gcctgaaggc gagcagacaa
     7801 atactcagta attgtgatat aattccccca tgaacaaaaa gcgaaaagtt gcagcaaaaa
     7861 agaaacggaa gaaggaaggg aaaacggttt ccttgaggca tcgttaaata acctgtggtg
     7921 agataccgaa ccataagaaa acccgagctt ttctcgggtt ttcttattgc aaattcgtgg
     7981 taaagtatga gtatggcgaa ggattactac aacattttag gtgtgcaaaa aggcgcctcg
     8041 aaagacgaga tcaagaaggc gttccataaa ctggcacacc agtaccatcc tgacaaacag
     8101 cacggtgacg aggcaaagtt taaagaggta aatgaggcat accaggtgct ctctgatgac
     8161 caaaagcgcg cgcagtacga caactttggt tccagttttc agcaaagtgg cggggctggt
     8221 ccgggtggat tcgactttaa tggatttgat ttttcaaact ttcaaaatgg gcaagggttt
     8281 gattttgacc ttggcgatat ttttggcgac ttctttggtg gcggcaaacg cgggcgagca
     8341 cgcaccgagc gggggcggga cttgcaggta gaagtaaagc ttacgtttaa agaagcaatc
     8401 tttggtgctg aaaagccggt aacggtaaag cgcaccgcaa cgtgcaaaac ctgcagcggc
     8461 agcggtgcaa agccgggtac taatttaaaa acctgctcaa aatgtaacgg gcatggcacc
     8521 attcgcgaca tgcagcgcac tatctttggc tcagttgcca ccacacgcac ctgtgatgtc
     8581 tgcagcggtg ccggaaagat tcctgagcat ccgtgtgcaa cctgtaaggg cactgggctc
     8641 actaaagaca cgagcacact caacgtggtt atcccgcctg gggtacaaca tggcgagatg
     8701 ctccgcttag gaaatatggg agaagcggtc cggggtggag aaacaggcga cctgtacctg
     8761 caaatcaccg ttgagccaca taaaactatt atccgcgacg gtaatcggtt ggttaccaca
     8821 ctcgatatca agctcaccga tgccctccta ggtgcccagt acgcggtaga gacgctcgat
     8881 gggagtattc cagttactat ccccgccggc acaaaaattg ggagcattgt aacgctcaag
     8941 aaccatggtg tgccgtcgcc ccagggcaag cgcggtgatt tcctcattaa gctcaacatc
     9001 aaactccctg aaaagatttc ttcaaaagca aaagcgatta tcgagactct gaaagaagag
     9061 gggatttaaa acatttattg atacaatact gcaatgtcat ctattgaaga ggtacgctct
     9121 gaacgaataa aaaaattagc agctttaaaa gagaaaggta ttgacccata cccagctgtt
     9181 tctcatcgca cctactctat tgcgcgattt caatcagaat ttgcagcact tgctgatgca
     9241 aagcagccag taacgattgc tggtcgaata atgagcaagc gcgggcaggg cggcgttacc
     9301 ttctgcgaca tatttgacgg caccagtaaa acgcagattt ttttaaagtc tgatgaaatg
     9361 gacgcagcac agtacgcgtt gtttacagaa acagttgatg tgggcgattt tttagaagcg
     9421 agtggtgcgg catacctcac taagcgcggt gagcagagtc tttttgtttc aaattggagc
     9481 atgctcacca agagcctccg tcccatcccc gatgct
//
LOCUS       DATIBQ010000074         3751 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U16294
            k141_1540660, whole genome shotgun sequence.
ACCESSION   DATIBQ010000074 DATIBQ010000000
VERSION     DATIBQ010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935789
            Sequence Read Archive: SRR25158331
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3751)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3751)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:58:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 637
            CDSs (total)                      :: 603
            Genes (coding)                    :: 601
            CDSs (with protein)               :: 601
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3751
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1540660"
                     /isolate="SMAG_U16294"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1560)
                     /locus_tag="VLB02_02490"
     CDS             complement(<1..1560)
                     /locus_tag="VLB02_02490"
                     /inference="COORDINATES: protein motif:HMM:NF012361.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HSE56928.1"
                     /translation="MEESTSQMDALPEKLLKPYNPPEEEGRIYAAWEGSGFFNPDTMV
                     KGGFTAPDAKPYTIMMPPPNVTGVLHMGHALMLTVEDILIRYKRMRGYRTLWLPGTDH
                     AAIATQSKVEKEIQKQEGKSRHDLGREEMLKRIEQFAKESHDTIVSQVKVMGASCDWS
                     REAFTLDAARTKAVQTVFKKMYDEGLIYRGHRIVNWDPKGQTTISDDEIVYEPRKAKL
                     YTFRYSKDFPIAISTTRPETKVGDVAVAVHPSDERYQAFIGKEYDVVFCGVPLHIKII
                     TDEAVEKEFGTGALGVTPAHSMIDWEIADRHDLPRPQVINEYAKMTVGDEHLLGKKTT
                     EAREAIIEWLRTEGLLESETETEQNVATAERTGGIIEPLPKLQWFINVNKPIPERGNK
                     TLKELMREPVAENQIKIIPDYFGKTYFHWIDNLKDWCISRQIWYGHRIPVWYKNTGQE
                     ITNKQAPISNEEIYCGLEAPTGDDWKQDEDTLDTWFSSALWTFSTLGWPDSAEASSGN
                     ARTGSDLDVYHP"
     gene            complement(1579..2793)
                     /gene="tsaD"
                     /locus_tag="VLB02_02495"
     CDS             complement(1579..2793)
                     /gene="tsaD"
                     /locus_tag="VLB02_02495"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:NF013012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061711 - N(6)-L-threonylcarbamoyladenine
                     synthase activity [Evidence IEA];
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HSE56929.1"
                     /translation="MKILSIETSCDETAVSVLECSGSTVRVLGNALSSQIDIHAQYGG
                     VFPALAKRAHAEKIIPLLQEALTEAELLYKTESNQPVVSFAEPDEETKQKIQTICEQE
                     PGLAEAIITFGKLYEAPDIDHIAVTTGPGLEPALWVGLNTAKALSVLWQKPIDAINHM
                     EGHVLSAVFQDSSLKEFTLTELPYPLLALLVSGGHTELVLTESPLHYKKIGATRDDAI
                     GEAFDKVARILGLPYPGGPKIAIFAKIAREANIESEIKLPRPMLHSKDYDFSFSGIKT
                     ATLYATKDKTLTDSEQKAVAREFEDAVVEVLVKKTAAAIEEFNIQTLVVGGGVAANTY
                     LREQLEIMRTKNFPHLTLSFPAKGLSTDNSVMIAMASYYRQLHGALLQDSPIIRAEGN
                     RSIESENFGLGL"
     gene            2862..3455
                     /locus_tag="VLB02_02500"
     CDS             2862..3455
                     /locus_tag="VLB02_02500"
                     /inference="COORDINATES: protein motif:HMM:NF012832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HSE56930.1"
                     /translation="MEELYIIVVGSSGAGKTRIAQLICEMYPKCMSSVSNTDRSPRGK
                     ETHAVDYYFRHPDKFDLMLTNDEFAEHNPNYVGKRYGTTKLELQRILDLGKIPVLDID
                     YVGAEQMRTLYGQKAYIVFIESPIELARKRLKERYDLSDEEIEGRLKRAVEEEIPFAY
                     SRPDVFDVIVENKENTVLDVLANQILNAALEKQKALV"
     gene            complement(3475..>3751)
                     /locus_tag="VLB02_02505"
     CDS             complement(3475..>3751)
                     /locus_tag="VLB02_02505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56931.1"
                     /translation="ITAPYEQREGWFLKGRNDGMQVLRTPEAFKEEERLVNSGTELWQ
                     ENVEETLKYAIKNFVNIEDKLQETIDNVIAFINEYVQLPRDTALNCY"
BASE COUNT          898 a          850 c          869 g         1134 t
ORIGIN      
        1 tgggtggtat acatccaaat cgcttcctgt tcgggcattg cccgacgaag cttcagcgga
       61 gtcgggccaa cccagcgttg agaatgtcca tagggcagat gaaaaccagg tgtcgagcgt
      121 atcttcatcc tgcttccagt catcacctgt aggtgcttct aatccgcaat atatttcttc
      181 attggaaatt ggcgcttgtt tattggttat ttcctgtccg gtattcttat accagacagg
      241 aatgcggtgc ccgtaccaaa tttgtcggct aatgcaccag tctttgaggt tatcaatcca
      301 gtggaagtac gtttttccaa agtagtcggg gatgatctta atctggtttt ctgctactgg
      361 ttcgcgcatc agctccttca gtgttttatt tccgcgttca ggaataggct tattcacgtt
      421 tataaaccac tgcagcttgg ggagtggctc aatgatgccg ccggtgcgct cggcggtagc
      481 tacattctgt tcagtttctg tttcgctctc gagcaagcct tcggttcgga gccactcaat
      541 aattgcttca cgcgcctcgg ttgttttttt gcccaagagg tgctcatcac cgaccgtcat
      601 ttttgcgtac tcgttaatta cctgcgggcg gggaaggtcg tgtcgatcgg caatttccca
      661 gtcaatcatg ctgtgtgcgg gggttacacc cagcgcacca gtgccaaatt ctttttcaac
      721 tgcttcatcg gtaataattt ttatgtgcag tggtacgccg caaaaaacca catcgtactc
      781 ttttccgata aatgcttggt atcgctcatc gctcggatgc accgctactg caacatcacc
      841 cacttttgtt tcagggcggg tagtagaaat tgcgataggg aaatcttttg aatatctaaa
      901 ggtgtagagc tttgctttgc gtggttcata tacgatttca tcgtctgaaa tagtggtttg
      961 ccccttcggg tcccagttta caattcggtg cccgcgataa attaaaccct catcatacat
     1021 ttttttaaat accgtttgca ctgcttttgt gcgagctgca tccaaggtaa atgcctcgcg
     1081 gctccagtca cacgaagcac ccatcacttt tacttggctt actatggtgt cgtggctctc
     1141 tttggcgaat tgttcaatac gcttgagcat ttcctcgcga cctaggtcgt gccgtgattt
     1201 tccttcctgc ttttgaattt ctttttcaac cttcgattgc gtggcaatgg cggcgtggtc
     1261 ggtgcccggg agccacaggg tgcggtaccc ccgcatgcgc ttgtagcgaa ttaaaatatc
     1321 ttcaacggtg agcatgagcg catgacccat gtggagcacg ccggttacat tcggcggcgg
     1381 catcataatc gtgtacggct tcgcatcggg tgcggtaaag ccgcccttaa ccatggtgtc
     1441 aggattaaaa aaaccgctcc cttcccaggc ggcgtatatc cggccttctt cttcaggcgg
     1501 gttgtagggt ttgagcagct tctcggggag ggcatccatc tgcgaagtag attcttccat
     1561 ggcaaaaata taccattttt aaagccctaa accaaaattt tcactttcta tagaccggtt
     1621 tccttcagct cggattatag gagaatcttg gagtagagcg ccgtggagct gccgatagta
     1681 gcttgccata gcaatcatca ccgagttatc agttgagaga cctttggcgg ggaacgagag
     1741 tgtaaggtgg ggaaaatttt tagtgcgcat aatctcaagc tgttcccgta gataggtgtt
     1801 tgcggctaca ccgccgccaa caacgagtgt ttggatatta aattcttcaa ttgcagcagc
     1861 agttttttta acgagtactt ctaccactgc gtcctcaaat tctcgcgcaa ccgctttttg
     1921 ttcactatcg gttagtgttt tatctttagt tgcatagagg gtggcagttt taattcccga
     1981 gaacgaaaag tcgtaatctt ttgaatgcag catagggcgg ggcagtttga tttcactttc
     2041 tatatttgcc tcgcgggcaa tttttgcgaa aattgctatt tttggtcctc ctgggtacgg
     2101 cagccctaaa attcgagcca ctttatcgaa tgcttcacca atggcatcat cgcgggtagc
     2161 gccaattttt ttgtagtgga gcggagattc ggtcagtact aattctgtat gcccgcctga
     2221 aactaagagt gccaagagtg ggtacggtag ctcggtaagg gtaaattctt tcaagctgct
     2281 gtcttgaaat actgccgaga gtacatgtcc ttccatgtgg ttaatggcat caatcggctt
     2341 ctgccagagc accgagagcg ctttagcagt atttaatcca acccacagtg ccggctcaag
     2401 ccccggacca gtggttacgg caatgtgatc aatgtctggt gcttcgtaca atttcccaaa
     2461 agtaataatt gcctctgcaa gtcctggttc ctgctcgcat atagtttgta ttttctgctt
     2521 cgtttcctca tctggttcgg caaagctcac cacaggttgg tttgattctg ttttatatag
     2581 taattccgct tcagtaagcg cttcttggag tagaggaatg attttttctg catgagcgcg
     2641 tttggcaagg gcagggaata caccgccgta ctgggcatga atatcaattt gtgagctcag
     2701 cgcattcccg agtactcgaa ctgtacttcc gctgcactca agcactgaaa cagccgtttc
     2761 gtcacaactt gtctcgattg agaggatttt catgccgcta tcctagcata ttgttgactc
     2821 tttcgcattt tgtagtattg tttaaaaaaa actttttgat catggaagaa ctatatatca
     2881 ttgttgttgg ctcctctgga gcgggaaaaa caaggattgc acaattaatt tgtgaaatgt
     2941 atcctaagtg tatgtcctcg gtatcaaata ccgatcgctc tccgcgagga aaagagactc
     3001 atgctgttga ttactatttc agacatcctg ataaattcga tcttatgctc acaaatgatg
     3061 aatttgctga acataacccg aattatgttg ggaaacggta tggtactacc aaactggaac
     3121 ttcagagaat acttgatctc ggaaagattc cagtgctcga tatcgattat gttggtgccg
     3181 aacagatgcg aactttgtac ggtcaaaaag cgtatatcgt ttttatcgag agtcctattg
     3241 aactcgcccg aaaaaggtta aaagaacgtt atgatttgag cgatgaagaa atcgaaggac
     3301 gcttaaaacg agcagtggag gaagaaatac cctttgctta cagtcgaccc gacgttttcg
     3361 atgttattgt agagaataaa gaaaacactg ttctcgatgt tcttgcaaac caaattttaa
     3421 acgcagcatt agagaaacaa aaagcccttg tctaaacaac aggggcttta ctatttagta
     3481 gcaattcaga gcagtatctc ggggaagctg aacgtactca ttaatgaagg caatgacgtt
     3541 atcaattgtt tcctgcagtt tgtcctcaat atttacgaag ttttttatgg catatttaag
     3601 tgtttcctca acattctctt gccatagttc ggtaccgctg tttacaagcc gctcttcttc
     3661 tttgaaggct tccggtgttc gaagtacttg cattccatcg ttccgtcctt ttaaaaacca
     3721 accctcacgc tgttcatagg gagcagtaat a
//
LOCUS       DATIBQ010000075         7527 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U16294
            k141_704035, whole genome shotgun sequence.
ACCESSION   DATIBQ010000075 DATIBQ010000000
VERSION     DATIBQ010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935789
            Sequence Read Archive: SRR25158331
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 7527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7527)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:58:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 637
            CDSs (total)                      :: 603
            Genes (coding)                    :: 601
            CDSs (with protein)               :: 601
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7527
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_704035"
                     /isolate="SMAG_U16294"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1837)
                     /locus_tag="VLB02_02510"
     CDS             complement(<1..1837)
                     /locus_tag="VLB02_02510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HSE56932.1"
                     /translation="MKKIHSTLAEHVSWYGKWHNNKHHSVAHYAILIAFIGTLSFGIA
                     STIQVPQSEAQEVKQLKEELTAKNQEFSAAVMLYAQAQGTEQRARANTLKEIAQRRSD
                     SMTLAAANDPQAFIEHVLPEAVKAHMPADVLALVEAKTKMEGTLVIIHTDTPGEGTYQ
                     TTLETKGGKAYTVHTAGGSLKKISDGSTVSIEGYVLGYQLVTADASAATVLAAAPVNS
                     SVGEQKTLVMLVNFSDNPTQPWTPAQVNTMYFGTPTENPQSLNSYYRENSYNQTWYTG
                     AVAGWFTIEKSQLSSICSMTDLQTVIDPLAAAAGFDISAYGHKVYMYPTTYCTGLSTV
                     GAFPSRSLVPKAGSTDVGTYQMMYHELGHALGKGRQHARMELCGTKQIDVAANCTFTE
                     YGDPYDVMGASQSTDTTLSLITNTRLFNALGRILLSWIPPVRVQTITTDGVYTLSSLE
                     TAQSSGTQVFKIYKADSSGGSDFITYSAEPLKSYYYLSYRQPYGTFDGNLPNYVTGGT
                     SVHADSAVLDGTAGDGNNFNTAILDNMSFIDDVNKITVTQLSHTSSTATIQVAFTPGF
                     CPWPPLFLNTVGVLNNQPVQPGGQVAFTYGVTGSEDPRCGTNTFAL"
     gene            1993..2676
                     /locus_tag="VLB02_02515"
     CDS             1993..2676
                     /locus_tag="VLB02_02515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56933.1"
                     /translation="MKNKNTLFGAVMLALSVLVVSSAYAQESLVGAAVKVQTGSSIEL
                     GAGNSASVTAEETVNADAKKSAAAEKKEEADKKEAADDEDDTNGETTAAAHRSAVAIF
                     VQELLKVADRERGIGAEVRVIAQEQQASEDTTTTAVAKVETRSKIKTFFFGTDYKTLG
                     TLRSEMVKTEARIIKLKALIEKATSATAKATLTAQLEALQAAELKIQTFIEAHENTFS
                     LFGWAAKKK"
     gene            2767..3522
                     /locus_tag="VLB02_02520"
     CDS             2767..3522
                     /locus_tag="VLB02_02520"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HSE56934.1"
                     /translation="MKLITLNTWAGRLKEPFEQFLKEYAKETDIFCFQEIFNKYEEAE
                     TVAPRTNNEKNPNILQEIRETLPDFNSYFCPVLENVYGIAIFLKKGIEVIASGEVVLY
                     TNPNYDPKDESSDHDRKMQWVHIKEGRKDLLIMNTHGHWDASGKGDTPNRIEQSKAIN
                     AFIESVGSTPKILVGDFNLNPETESIKLLEKYFINMITKEGVATTRTELYTGADRHAD
                     YVFVSPEVFVNQFKVLPDVVSDHAPLFIDFDLF"
     gene            3718..4686
                     /locus_tag="VLB02_02525"
     CDS             3718..4686
                     /locus_tag="VLB02_02525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56935.1"
                     /translation="MKNIIQKIALFALLLAPIGAAVSVEAAAAPQWNTTGTYEVAFNY
                     LGADYGHDMALTQDSAGALTGSGGHPIGANTYTWVITSGTVSADAIDFSANYTASADA
                     VTPLTTMHVMGTIALDGTMSGTWSDNYQGGTRSGTWETTSGIAAPLGSLAAEDFGVVN
                     YDTGLGQLKGYTAGFGLTDATFEDVQSVVVQLYAGSTLLQTNTATAKVGTDITGNQIS
                     SPFDVSGTFDYATDGYWINVKESEYGQSVPATRVVATITLENGKVVTAENTQLTGDPT
                     TIFPPTPPPVVTPTTKEQCKNGGWKTFTSPSFKNQGQCVSSVASNK"
     gene            complement(4759..5682)
                     /locus_tag="VLB02_02530"
     CDS             complement(4759..5682)
                     /locus_tag="VLB02_02530"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HSE56936.1"
                     /translation="MRQRHTLFFILLVCLIAGAGFFVIQKNHSRPTNSLPASASISIE
                     TSTPEPQALPVLPTYHTVTLESTAPIAELTEKVGKEQVPVVLALNRIDTRHIQVGAVL
                     TVPDSSTDFLTLTPFPTNLPSVNEVPKIIFVSQKIQAFAAYEYGTLVRWGPVSTGKKS
                     TPTASKLYYTNWKGKLITSTIDDGWIMPWYFNLDNQEGISMHQYDLPGYPASHSCVRL
                     AEADAEWMYTWAEQWILDKKTGKKLANGTPTIVFGTYAYSETAPWKLLPTDQNATVVT
                     TEDLEETLAPYLSDIQASVENRIKILNPDSQ"
     gene            5916..>7527
                     /locus_tag="VLB02_02535"
     CDS             5916..>7527
                     /locus_tag="VLB02_02535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56937.1"
                     /translation="MMSAVRAFFSRYRRVLLPLLFLFLYSVGVSYAVPPTSKYQPGST
                     LDPACAPGSTNCSVEILPDQTGNVNKVLVTDGTTASWNSTITAVTLATPTIAGAASDP
                     TGADGLLYYNTATDTFKGYDGSWQDLATQNYVTTNHYTKTELSTADGSQVSWFNITNA
                     PIIGTNTDTRLIDGGQVSFVSGYTFLVSAANYQILGTSYSSPQSQVTLSASDPTNPRI
                     DVVAVNTSGAVVVIEGTPGVSPEQPYVDPETQLMLTFVNVAAASSDPGITTVDIYKEN
                     TGPAAEWSRSSNSGTISLNSSASPRNGTKNIEGTSVTSGAKLTFSPAAGFDINGTTQL
                     VLYVKSKATWGNNRKLSIAFQNASGQKGNAVLISKSGSFGFDSSQTSSYQQIAIPISN
                     FGVPSGTLITSMTITAIGSQGTFGFYIDDLTLESGSPIVGGAATTVATSNSSTITFTG
                     NGLVSNPLSASVNTTGLDATYFKQNGNSFAGLATVGTNDANALAFETSGSERIRILST
                     GNVSIGDTSPDQLFEVLSSGAALTQLSIG"
BASE COUNT         2052 a         1608 c         1817 g         2050 t
ORIGIN      
        1 tgagtgcaaa agtgttggta ccacagcgcg gatcttcact gcccgttact ccataggtga
       61 aggcaacctg tccccctggc tgtaccggtt gattattaag aacgcccaca gtatttagga
      121 agagtggtgg ccatgggcaa aagccaggag taaaggcaac ttgtatagtc gcagtgctgc
      181 ttgtgtgact gagctgagta actgtaattt tatttacatc atcaataaaa gacatgttgt
      241 cgagaatagc agtgttgaaa ttatttccgt ctcctgctgt tccatctaat acggcactgt
      301 cggcatggac gctggtgcct cctgttacat aattaggaag gttgccatca aacgttccgt
      361 agggctggcg ataactgagg taataataac tctttagtgg ttctgcagaa taggtaataa
      421 aatcagatcc tcctgaacta tctgctttat atattttgaa tacctgcgtt cccgatgatt
      481 gagcagtttc aagagaagag agggtataca ctccatcggt ggtaattgtt tgtacgcgta
      541 ctggcggtat ccacgataag agtattcttc caagtgcgtt aaagaggcgg gtgttggtaa
      601 tgagtgaaag agtggtatcg gtgctctgac ttgcgcccat cacatcatat gggtcgccat
      661 actcggtaaa ggtgcagttt gctgctacat caatttgttt tgttccgcag agctccatgc
      721 gtgcatgctg ccgccccttg ccaagtgcgt gcccaagttc gtgatacatc atttgatagg
      781 ttccgacatc agtgctgccc gctttaggga caagtgatcg cgaaggaaat gctccaaccg
      841 tagagagacc ggtacagtag gtggtcgggt acatgtacac cttatgacca taggctgaaa
      901 tgtcgaatcc ggcagctgcg gcgagcgggt cgatcacggt ttgcaaatca gtcatcgagc
      961 agatactgct caattgtgat ttttcgatag taaaccagcc ggcaaccgct ccggtgtacc
     1021 aggtttggtt gtacgaattt tcacggtagt acgaattgag cgattgcggg ttctctgttg
     1081 gtgttccaaa gtacatcgtg ttcacctgtg ctggtgtcca cggctgggta gggttgtctg
     1141 aaaaatttac gagcatgacc aatgtttttt gttctccaac cgatgaatta acaggggcag
     1201 cagcaagcac tgttgctgcg ctcgcatctg cagtaacaag ctggtatccg agcacgtagc
     1261 cttcaatcga aacggtagag ccgtcactta tttttttaag tgatccacct gcggtgtgta
     1321 ccgtatatgc ttttcctccc tttgtttcca gggtggtttg gtaggtgcct tccccggggg
     1381 tatcggtatg tataatcacg agcgtgcctt ccatttttgt ttttgcctca acaagtgcta
     1441 atacatcagc aggcatatgc gcttttactg cttcaggcag aacgtgttca atgaaggctt
     1501 gcgggtcatt cgctgcagcg agagtcatac tgtccgagcg gcgctgtgca atttctttta
     1561 aggtatttgc ccgggcacgc tgctctgtcc cttgtgcctg agcatagagc ataaccgcag
     1621 cagaaaactc ttgattttta gcagtaagct cttcttttag ctgctttact tcctgtgcct
     1681 cggattgtgg tacctgtatg gtgcttgcga taccaaaaga gagtgtgcca ataaaagcga
     1741 taagaattgc gtagtgtgca acactgtgat gtttgttgtt atgccacttt ccataccaag
     1801 aaacgtgctc tgcgagagtt gaatgtattt ttttcatcgt aaatttacta tggtaataaa
     1861 aataaaaaac aaaaaagatg tactcgtcct gtacttatac tgaagtatag gctaaaaaga
     1921 gaagaactgc ccggctggca tgtgtaaaag tgtcttgagt atagttggta tcctttgtta
     1981 tacttaagag atatgaaaaa taaaaacaca ctctttgggg cagtaatgtt agcactttca
     2041 gttctcgtag tttcatctgc gtatgcacaa gaaagcctag taggtgctgc ggtgaaggta
     2101 caaactggct cgagtattga acttggagct ggaaatagcg cttctgttac cgcagaagaa
     2161 acagtaaacg ccgacgcaaa aaagtcagcc gctgcagaga aaaaggagga agctgataaa
     2221 aaagaagcag ctgatgacga agatgacaca aacggcgaaa caactgctgc agcgcaccga
     2281 agtgcggtag cgatatttgt gcaggagctc ttgaaagttg ccgatcgcga gcgcggtatc
     2341 ggtgcagaag ttcgagttat tgcacaagag cagcaagcgt ctgaagatac caccaccacc
     2401 gccgttgcaa aagtggaaac tcggagcaaa attaagacct tcttttttgg aaccgactat
     2461 aaaacactcg gtaccctccg cagcgaaatg gtaaaaactg aagcgcgaat tataaaactg
     2521 aaagcgttga tcgaaaaagc aaccagtgct acggcaaaag caacactcac tgcgcagctc
     2581 gaagcgctcc aagcagcaga gcttaaaatc caaacgttta ttgaagcgca tgagaacact
     2641 ttcagcctct ttggatgggc tgcaaaaaag aaataaactg tttcttattg attaatttaa
     2701 aaaaagccca gactgggctt ttttggtttt agaaagcatt ttgtagagca atgctataat
     2761 tactgcatga aactgattac gttgaatacc tgggcaggga ggctcaaaga gccgtttgag
     2821 cagtttttga aggagtatgc aaaagaaaca gacatatttt gctttcaaga gatttttaac
     2881 aagtatgaag aggcggagac tgtggctcct cgtactaata atgaaaagaa tccaaacatt
     2941 cttcaggaaa taagagaaac actgcccgac tttaattcat acttttgtcc ggtgcttgaa
     3001 aatgtctacg gtatcgctat ttttttgaaa aaagggattg aagttattgc aagtggagag
     3061 gtagtgctgt acaccaaccc aaattatgac ccgaaagatg aatcgagcga ccatgatcgc
     3121 aagatgcagt gggtgcacat taaagagggc cgaaaggatc ttcttatcat gaatacccat
     3181 ggacactggg atgcctcagg aaagggtgat acgccaaacc gcatcgagca atcaaaagcc
     3241 attaatgcat ttattgaatc agttggttct acaccaaaaa tcttggttgg tgacttcaat
     3301 ctgaatcctg aaacagagag tattaagctg cttgaaaaat attttatcaa tatgatcacc
     3361 aaagaaggtg ttgctactac ccgcaccgaa ctctacaccg gagcagatcg gcatgctgat
     3421 tatgtgtttg tttcgcccga ggtatttgtg aaccagttca aagtgctccc tgatgtagtc
     3481 tcagaccacg caccgttatt tattgatttt gacttgtttt gaattgacga acaatttcta
     3541 tgccacagta aagtaatctg tctaggtaaa gaagggcggg gtaagtcgtc ttacaggcga
     3601 agggcaatat aatcgtactt tgaaaaagga ttaagaatag aatttgtaac gatttagtac
     3661 aacacgagtt tgctagctct ttatagattc agattataag ctaatcaata aaaaaatatg
     3721 aaaaacatta ttcaaaaaat tgcgcttttt gcgttgctac tcgcgccaat aggtgcggcg
     3781 gtatcagtag aggcagcagc agctccccag tggaatacta ccggaactta tgaagttgca
     3841 ttcaactact taggagcaga ttatgggcac gatatggctc tgacccaaga tagtgcgggt
     3901 gcgcttacgg ggagcggcgg tcatcctatt ggagcgaaca cctacacttg ggtgataacg
     3961 tctggaacag tatctgcaga tgcaatcgac ttctctgcga actacactgc gtcggcagat
     4021 gcagtaacac cacttactac tatgcatgta atgggcacca ttgcgctcga cggaactatg
     4081 tcaggtacct ggtccgacaa ctaccagggc ggcactcgga gcggtacctg ggagacaact
     4141 tcaggtattg cagcaccgct cggcagcctt gcagcagaag actttggggt tgttaactac
     4201 gacactggtc ttggacagct gaaggggtac accgcaggat ttgggttaac cgatgcaacg
     4261 tttgaagacg tgcagtcagt ggtagtacag ctctacgctg gaagcacact cttgcaaaca
     4321 aatactgcaa ccgcgaaagt gggaacggat attaccggca accaaatttc ttcaccgttt
     4381 gatgtatcgg gaacgtttga ttacgctacc gatgggtatt ggatcaatgt aaaagaaagc
     4441 gagtatggcc agagtgtgcc tgcgacacgt gttgttgcaa cgattaccct ggagaatggc
     4501 aaggtggtga ccgctgaaaa cacgcagctc actggagacc ctaccactat ctttccacca
     4561 acacctcctc cagtagtaac accaactaca aaagagcagt gtaagaatgg cggatggaag
     4621 acattcacca gtccaagctt taagaatcag gggcagtgtg taagttcagt ggcaagcaat
     4681 aaataaattg ctgaataagc attagtttga caaagagcac ccttgatggg tgctctttgt
     4741 atggaagctt ttataaaatt actgagaatc aggattaagt attttgatac gattttctac
     4801 acttgcttgg atatcagaga ggtacggtgc cagtgtctcc tcaagatctt ccgtggtaac
     4861 aacggtagca ttttgatcgg tgggaagcag tttccacggt gcggtttcgc tatacgcata
     4921 cgtgccaaaa acaatagttg gagtaccatt tgcgagcttt ttgccggttt ttttgtccag
     4981 aatccactgc tccgcccagg tgtacatcca ttctgcatct gcctcagcga gtcgtacaca
     5041 cgaatggctt gccgggtacc cgggaaggtc gtactggtgc atcgatatcc cctcttggtt
     5101 atctaagtta aagtaccagg gcataatcca cccatcatct atggtgctcg taataagctt
     5161 tcctttccag ttggtgtagt agagcttgct tgcggttggg gtacttttct tgccggtgct
     5221 cactggtccc cagcgcacca gggtgccgta ctcataggct gcaaatgctt ggattttttg
     5281 agagacaaaa ataatttttg gtacctcatt tactgatggt aggttagtgg ggaatggagt
     5341 aagtgtcaaa aaatcagtac tagaatcagg aacagtaagt actgctccga cctgtatgtg
     5401 ccgagtatca atacggttta aggcaagtac cacgggcacc tgctctttac ctactttttc
     5461 tgttaattca gctataggtg ccgtactttc cagggtaacg gtatggtaag tgggtagtac
     5521 tggtagtgct tgtggttcgg gggtcgatgt ttcaatagag attgaagcag aggcaggaag
     5581 ggaatttgta ggacgactgt gatttttttg gatcacaaaa aaaccagccc cggcaatgag
     5641 gcacacaagc agtataaaaa atagagtatg acgctgtcgc atcctctcgc cttactttct
     5701 cctaagcgcc tttttgcgag catcagccgc ttcgcttcga gccgtttttt cgtttttgga
     5761 gaatgctttg atttcttttt agggcgcagt gccgtacttc tttttgccat atgtattatt
     5821 gcttaatcag taatgcttcc attatgcact atgtttcaat aaatagctct ttttctacag
     5881 tttttattgc tgcactatag tataataatt ggtacatgat gagcgcagtg cgggcatttt
     5941 tttcacgata tcggcgtgta ttactcccgc ttctttttct ttttttatac agcgttggtg
     6001 tttcgtatgc ggtgccgccc acttcaaagt atcagcctgg ttcaacgctt gatccggcat
     6061 gtgcgcccgg atcaacaaac tgctcggttg aaattttgcc ggatcaaaca ggaaatgtaa
     6121 ataaagtatt agtaactgat ggaacaactg cttcatggaa ttctactatt actgccgtaa
     6181 cgcttgctac accaactatt gctggagcag cttccgaccc gacaggtgct gatggtttac
     6241 tctattacaa tacagcaact gataccttca aaggctatga tggttcatgg caagatttgg
     6301 cgacgcagaa ttacgttacc accaatcact acaccaaaac agaactctcc actgccgatg
     6361 gatcgcaagt cagttggttt aacataacca atgcgccaat tatcggaaca aacactgata
     6421 cacgacttat tgacggtggg caggtatcat ttgtttctgg atatacgttc ttagtttcag
     6481 cggctaatta tcaaatcctc ggtacatcgt attcatcacc gcagtcgcag gtaacactct
     6541 ctgcttctga tccaaccaat cctcgcatcg atgtagttgc ggtgaatacg agtggggcgg
     6601 tggttgttat cgagggtacg ccgggtgtta gcccagagca gccgtatgtt gatcctgaaa
     6661 cgcagctgat gctcacattt gtgaatgtcg cagcagcaag ttctgatcca ggaataacaa
     6721 cggtagatat ttataaagaa aataccggtc cggctgccga gtggagccgc agttcaaata
     6781 gcggaacaat ttctttgaac agttcggcga gtccgcgcaa tggtacaaaa aatattgaag
     6841 gaacgagtgt tacttctggt gcaaagctta ctttttctcc agcggcaggg tttgatatta
     6901 acggaacgac gcagcttgtg ctgtatgtga agagcaaggc gacgtgggga aacaatcgaa
     6961 aactctctat tgcctttcaa aatgcaagcg gacagaaggg gaatgcggta cttatcagca
     7021 aaagcggctc atttgggttc gatagctcac aaaccagtag ctaccaacaa attgccattc
     7081 ctatcagcaa cttcggtgtt ccttcaggca cccttattac ttcaatgacc atcactgcaa
     7141 ttggcagtca aggaacattt ggtttttata tagatgacct cacccttgag agcggtagcc
     7201 cgattgtggg cggtgcggca acaacggttg ctacttcaaa ttcttccacc atcacgttca
     7261 ccggaaatgg tctcgtctca aacccgctct cagcaagtgt aaatactact ggtctcgatg
     7321 ctacgtactt caaacagaat ggtaattcat ttgccgggct tgctacagta ggcaccaacg
     7381 atgcaaatgc gttggcattt gaaacaagcg gtagtgagcg aatacgaata ctcagtacgg
     7441 gcaatgttag tatcggtgat acttctcctg accagctgtt tgaggttctc tcatctggtg
     7501 ctgcgctcac acaactctct attggca
//
LOCUS       DATIBQ010000076         4131 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U16294
            k141_758700, whole genome shotgun sequence.
ACCESSION   DATIBQ010000076 DATIBQ010000000
VERSION     DATIBQ010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935789
            Sequence Read Archive: SRR25158331
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 4131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:58:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 637
            CDSs (total)                      :: 603
            Genes (coding)                    :: 601
            CDSs (with protein)               :: 601
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4131
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_758700"
                     /isolate="SMAG_U16294"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1248
                     /locus_tag="VLB02_02540"
     CDS             <1..1248
                     /locus_tag="VLB02_02540"
                     /inference="COORDINATES: protein motif:HMM:NF025253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HSE56938.1"
                     /translation="LADGTNTFTLGVDDSDGDKFKISTTALGTNDRFVIDGSGNVSIG
                     DASNYTGIDPDGDLQFQGTADYLVAGNEYAFRFVTDEDYGLKFDSTNGVYAFMDSTAV
                     RQFSINADTGATAIGNTNTGFMLEVGSASIVGVVARFVNSTGTCDINPTTTSLSCSSD
                     ATLKRDIETYTNALDRINQLRGVTFSWLSDTAVDPRRIGFIAQEVESVIPELVATDAA
                     GKKSVAYANLAPVLVEAVKELDLKISSFDSLQAAAGNSLLADGMAADNFTEKVKLSFI
                     KLSDVLLDMKLHVQELFTKKVTTSELCLDDLCVTKSELQELLNLRSEGTPPPPPASDE
                     VSNNGDFPPTDDTPEDSDQDSGEEPTPNTETPPEEEVLGEQNEPDNLESGNELEASEG
                     EAPENEPKPAQDSAPTEPLEGSQ"
     gene            1319..3376
                     /locus_tag="VLB02_02545"
     CDS             1319..3376
                     /locus_tag="VLB02_02545"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase subunit B"
                     /protein_id="HSE56939.1"
                     /translation="MAKEGKKGSYGAEQISVLEGLEPVRRRPGMYIGSTGIDGLHHLV
                     WEIFDNSRDEAMAGHANHIEVVLLPGNRIRVADNGRGIPTGIHPKTKVSALETVMTTL
                     HAGGKFGGDESGYKVSGGLHGVGASVVNALSIYTRAEVHREGDIFVQEYSQGNKKANV
                     KKIGKTEHNGTIITFEPDIEIFKEIRFDWKTVIDRLRQQAYLVKALRVTIIDARAVEE
                     KFDPTNTFWFKELGISAPSHTYYFEGGLVSLVAHYNAHQKPIHETIFYCEKEVDNVGV
                     EIALQYVDDITPRITAFANNIYNLEGGTHVTGFKMALTRLINTYAKKNNLAKEADGAF
                     SGDDVLEGLTAVVSVKLAEIQFEGQTKAKLGSTEAQGATATAFGDAFNTFLEEHPDEA
                     KAIVMKVLLALRARKAAKAAKDSILRKGALEGMTLPGKLADCQTKDASEAEVFIVEGD
                     SAGGTAKQGRDRHFQAILPLRGKILNVERARLDKMLASQEIKNLVIAMGTSIGDTFDI
                     ARMRYHKLIIATDADVDGSHITTLLLTLFFRYFREVIDAGHVYIALPPLYKIKKGKEI
                     FYAFSDEEKRGIIGNVAMDEVQEVEPGEGAAEGEEGEEAEEKGKTKVPKISVQRYKGL
                     GEMNASELWETTMDPKRRVIKQVTIEDAEEANQTFDMLMGSDVAPRKSFIQSNAKLAN
                     LDI"
     gene            3518..>4131
                     /locus_tag="VLB02_02550"
     CDS             3518..>4131
                     /locus_tag="VLB02_02550"
                     /inference="COORDINATES: protein motif:HMM:NF021665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G8 domain-containing protein"
                     /protein_id="HSE56940.1"
                     /translation="MNIRITKAPLVMFLFIFFTSLFSVSFHAFAHDMPGCEISGTITS
                     VQSGNWSNPATWGGRVPGTADTVAILTHHTVVYDMDTATIAGVNLACDAILQFDPTKN
                     TTLQSTKNIVIKGTLEMHPNTSAVQQTIQFIGINEDTFIGGGENPLDSDIGLWVMGTY
                     GKADLLGTQKTPWLNASGALSAGATTITLSAVPVGWRVGDEVSIA"
BASE COUNT         1185 a          854 c         1035 g         1057 t
ORIGIN      
        1 ctcgctgatg gtactaatac cttcaccctc ggtgttgatg attcagacgg agataaattc
       61 aagatttcaa caacggcgct tggtacgaat gatcggtttg tgattgatgg gagcgggaat
      121 gtgtcgatcg gcgatgcttc caactataca gggatcgatc cggatggtga tctccaattt
      181 caaggtactg ccgattactt agttgcaggc aatgaatatg cattccgttt tgttaccgat
      241 gaagattatg gtttgaaatt cgattcaaca aatggtgtat atgcatttat ggatagtaca
      301 gcagtgcgac agtttagtat aaatgctgat actggcgcta ctgctattgg caatacaaat
      361 accggcttca tgcttgaggt gggtagtgcc agtattgtgg gtgttgttgc ccgctttgtg
      421 aattcaaccg gtacctgtga tatcaacccg acaacaacct ccctctcctg ttcttctgat
      481 gcgaccctga aacgtgatat tgaaacatac actaatgcac ttgatcggat caatcagctg
      541 cgaggtgtta cctttagttg gctgagcgat actgcggttg atccgcgccg catcggtttt
      601 attgctcaag aagttgagtc ggttatccct gaactggttg caacagacgc ggcaggaaaa
      661 aaatcagttg cctatgcaaa ccttgctccg gtgctggttg aagcggtgaa ggagcttgat
      721 ctgaaaataa gctcatttga ttcactacag gcagctgccg gtaactcgct tctggctgac
      781 ggtatggctg ccgataattt taccgaaaag gtgaagctca gttttataaa actttctgat
      841 gtgctgcttg atatgaagct gcacgtacaa gagttgttca ctaagaaagt aactacttct
      901 gaactctgtt tagatgacct gtgtgtaacc aagagtgaat tgcaggagct gcttaattta
      961 agaagcgagg gcacccctcc tcctccgcca gcttctgacg aagtatctaa taatggcgat
     1021 tttccaccca ctgatgatac tccagaagat tctgatcagg atagtggaga agagccgact
     1081 cctaatactg aaactcctcc agaggaagag gtgcttggtg agcagaatga accagataat
     1141 ttagagtcgg gtaatgaact tgaagcttct gaaggtgaag cgcctgaaaa tgagccaaaa
     1201 ccagctcaag attctgcacc cacagaaccc ttggagggga gtcaataatt tgctatacta
     1261 gaggggtaaa aaacaaagaa aaagccttat aaaaaagcgc ttttttatat acacacgcat
     1321 ggcaaaagaa ggaaagaaag gatcatacgg cgcagagcaa atttcagtac tggaagggct
     1381 tgagccagtg cgccgccgcc ccggcatgta cattggttct actggtattg atggtttgca
     1441 ccatttggtg tgggagattt ttgataactc acgcgatgag gcaatggcag ggcatgccaa
     1501 ccatattgag gtagtacttt tgccaggcaa ccgtatccgc gttgccgata acggtcgcgg
     1561 catccctacc ggaatccatc caaaaacaaa agtttctgct ttagaaacag tgatgaccac
     1621 gctccatgca ggtggcaagt tcggcggcga tgagagtggg tataaagttt ctggcgggct
     1681 ccacggtgtg ggtgcatccg tagtcaatgc actctcgatt tacacacgcg ctgaagtaca
     1741 ccgtgagggt gatatttttg ttcaagaata ctcacaggga aataagaagg cgaatgtaaa
     1801 gaaaattgga aaaacagaac acaacggtac cattattacg tttgagccgg acatagaaat
     1861 ttttaaagaa attcgcttcg attggaaaac agtaatcgat cgcttgcgcc aacaggcgta
     1921 cttggtgaag gcactccggg taactattat tgatgctcga gcagtagaag aaaagtttga
     1981 tccgaccaac accttttggt ttaaagaact cggtatttca gctccgtcgc acacgtacta
     2041 ttttgagggt gggttagtgt ccctcgttgc gcactacaat gcacatcaaa aaccaatcca
     2101 cgaaactatt ttttactgtg aaaaagaggt tgataatgtt ggtgttgaaa tcgcgctcca
     2161 atatgtcgac gacataactc cgcgcattac tgcgtttgca aacaatattt ataacctcga
     2221 ggggggtacg cacgttactg gttttaagat ggcgctcacg cggctcatca atacctatgc
     2281 gaagaaaaat aacttggcaa aagaagctga cggtgcgttc tcgggtgatg atgtgcttga
     2341 ggggctcacg gcagttgttt cagtaaagct cgctgaaatc caatttgaag gacagaccaa
     2401 ggcaaagctc ggttcaactg aagcgcaggg agcaacagca accgcctttg gcgatgcgtt
     2461 taatacgttt cttgaagagc atcctgatga agcaaaagcg attgtgatga aagtactctt
     2521 ggcgcttcgc gcccgcaaag cagcaaaggc agcaaaagat tcaattttgc gaaagggggc
     2581 acttgagggg atgactttgc ccggaaaact ggctgactgt caaaccaaag atgcaagtga
     2641 agcagaggta tttattgtag agggagactc tgcaggcggt actgcaaaac aggggcgtga
     2701 ccgccacttt caagcaatct tgccgttgcg cggaaagatt ttaaatgttg agcgtgctcg
     2761 gctcgacaaa atgctcgcct cgcaggaaat caaaaatttg gtaattgcca tgggaacatc
     2821 gattggagat acgtttgata ttgcacgtat gcggtaccac aagctcatta ttgcaaccga
     2881 tgccgatgtt gacggctcgc acattaccac ccttttattg acgctgtttt tccgctactt
     2941 ccgcgaggtg attgacgcgg ggcatgtcta catcgccctg ccaccactct ataaaatcaa
     3001 gaaagggaaa gaaatttttt atgcattctc ggatgaagag aaacgcggca ttattggaaa
     3061 tgttgccatg gatgaagtac aagaagttga accaggcgaa ggtgctgctg agggagagga
     3121 aggtgaagaa gcagaagaga aaggaaaaac aaaagtacca aaaataagtg tccagcgata
     3181 caaagggttg ggagaaatga atgcgagcga gctctgggaa acgactatgg atccaaagcg
     3241 ccgggtgatt aagcaagtaa caatcgaaga tgccgaagag gcaaaccaaa cctttgatat
     3301 gctgatgggg agcgatgtgg cacctcgcaa gagttttatc caatcaaatg caaagctcgc
     3361 aaatttagac atatagtaga ggccccgcga gaggcttttt agttttcccc tactctctat
     3421 atcttgcgta ttctaaaaat tatacaatac aagtagtctg tgtactacag atccgctatt
     3481 accaatctta gactatttct tcagtgataa aatttttatg aatatacgta ttaccaaagc
     3541 acctcttgtg atgttccttt ttattttttt tacatcgctc tttagtgtct catttcatgc
     3601 ttttgcacac gatatgcctg gctgtgaaat ttccggcaca attacgagcg tgcaaagtgg
     3661 taactggtca aaccctgcaa cctggggcgg gagagtgccg gggacagctg atacggtggc
     3721 tatactcact caccacacag ttgtgtatga catggatacc gcaaccattg cgggggtaaa
     3781 tcttgcctgt gatgcaatat tgcagtttga tccaacaaag aatacaacac tgcagtctac
     3841 caagaatatt gttattaagg gaacattaga aatgcaccca aatacctcgg ctgtgcagca
     3901 gactatacag tttatcggta tcaatgaaga tacatttatt ggcggcggag aaaatccact
     3961 tgattctgat atcgggctat gggtgatggg gacatatgga aaagccgatc tgcttggtac
     4021 tcaaaaaaca ccgtggctca atgcgagtgg tgcactcagt gcgggagcaa ccactattac
     4081 gctcagtgct gtgccagtag gatggcgggt gggtgatgaa gttagtattg c
//
LOCUS       DATIBQ010000077         4392 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U16294
            k141_1387359, whole genome shotgun sequence.
ACCESSION   DATIBQ010000077 DATIBQ010000000
VERSION     DATIBQ010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935789
            Sequence Read Archive: SRR25158331
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 4392)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4392)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:58:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 637
            CDSs (total)                      :: 603
            Genes (coding)                    :: 601
            CDSs (with protein)               :: 601
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4392
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1387359"
                     /isolate="SMAG_U16294"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3156)
                     /locus_tag="VLB02_02555"
     CDS             complement(<1..3156)
                     /locus_tag="VLB02_02555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56941.1"
                     /translation="MKKLLVLFLFLGISSVGFFLSPKEASTANPGVRTNTARAPITII
                     NYYNQQMGFYESLPKGYNDDPAREWPLIIFLHGDGERGNGTTELSRVLGAGLPKVINE
                     GAQLEYTVNGKLDSFVVIAPQLDAGSSEWHPYQVDAFIEYAKENYRIDTDRIYLTGLS
                     LGAFGAHRYPKFSPAFAQKIAAVVATDGADAGTSIYTPDYPGGMAPLDACFVPANNVK
                     TWFIWGDADGWIWTVNGALAALAACTPSAAPTKTTVYPGLGHGVWTQTYDPTNSYQTP
                     NTYEWLLAQTRGTAPTPTPVPGNQAPTASAGSDQAITLPTSTVTLSGSGADADGIITS
                     YVWTKVSGGAATIVSATTASTQVTGLVEGAYVFRLTVTDSSGGTATDEVQIAVTTAVT
                     LMDPAARRIVITPEMLFNETLVGNEEKLFNEQGTLVLDASGNPLQTTRPAACKGVWEE
                     PPVDRTCFHVNDTNSNKELAYPGKIIVDLGRTYKITDIYLYDDRGGTHAAGDTEPLQV
                     WTGGPLKYTQKIATHNFEKENEWVRLSVTPTETRYLQLRFNDGHGFIGEVVLYGVPMG
                     STPSTAAVPTIKPNYTFEKMIGTNISFGMGQDANGTKYDDQWGTVRYFGGLRWTFDKN
                     DNLTPLDGIALETITRMVQKGVGVYSVLMGAAWTMVDPTDPLYISEAYTSFSDRKAID
                     YTLCAGSKIVGGLPSCDTVAQDPASYAHKAWLSKRVAKRDYDLGVRMIELGNEPDGFW
                     QNAGYYLPFETAAMVSADYDGHCNTLRYRGETVGVKNAAPGMKVVLPAVSQHDPRYLK
                     AMEFWFEHNRPCAPTFPADVINFHTYATTLGYQFAGNTGVSMPPEAFGIREQSESFVQ
                     FRNRYAPHAEVAMSEFGVDTYYEQHPNPTHPWCGSTVYGNSWAYAPKVGPFDEKEVQG
                     QWLARSYLEQAAAGLDFVNQFWLSDQYREEESCFTFTTAGVLRITNVTDYKPTYEPKV
                     SWYYTQTLRTRLAGMQYESEKNVSNGLGTGTIRIMKFRSVATPSAVAYVLWSPTANNV
                     TNPNFVLSLDTAASASVIELTK"
     gene            complement(3385..3456)
                     /locus_tag="VLB02_02560"
     tRNA            complement(3385..3456)
                     /locus_tag="VLB02_02560"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3421..3423),aa:His,seq:gtg)
     gene            complement(3519..>4392)
                     /locus_tag="VLB02_02565"
     CDS             complement(3519..>4392)
                     /locus_tag="VLB02_02565"
                     /inference="COORDINATES: protein motif:HMM:NF019499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HSE56942.1"
                     /translation="SRFSKTLGARNEVLGMGYYLLVWALYAAKLFVPAIATPLYDLFL
                     VLVTAGAFFFSLYLTAVQAFILKSWCTWCIASALCNTILFTLIFFTVPLAPVVALLGQ
                     YRLAALIMHNIGFILGLGAATITDILFFRFLKDHHISEDEKGTLDTLSSVIWVGLTLL
                     VFSGLALYLPESARLNESPKFLLKAVVVGVVLVNGFLLNFKVAPYLTQLSFDRAKAPT
                     HLRRLAFALGGVSIVSWYTAFFLGSLRTIKVEFEHGLFIYFGLLVVSTIGSQVFERFS
                     VRKEPSPLPEAVNR"
BASE COUNT         1105 a         1137 c         1026 g         1124 t
ORIGIN      
        1 tttcgtcaat tcaatcactg atgcagaagc agctgtatcg agagagagta caaagttcgg
       61 gttagttaca ttgtttgctg ttggcgacca gagcacgtat gcaactgcgc ttggtgttgc
      121 gacacttcta aatttcataa tccgtattgt tcctgttccg agcccatttg aaacattttt
      181 ttcactctca tactgcatgc cggcaagccg ggtacggagc gtttgggtgt agtaccacga
      241 cacttttggt tcataagtcg gcttgtagtc ggtaacattg gtaatccgta acactcctgc
      301 ggtggtaaac gtaaagcacg attcttcttc tcgatactga tcagagagcc aaaactggtt
      361 tacaaaatcc aaccctgcag ctgcctgctc gaggtatgag cgcgcaagcc actgcccctg
      421 tacttctttt tcatcaaacg gaccaacctt tggcgcatat gcccaggaat ttccgtagac
      481 agtgctccca caccacgggt gtgttgggtt gggatgctgc tcgtaataag tatcaacacc
      541 aaattcactc atcgctactt ctgcatgtgg cgcatagcga tttctaaatt gcacaaacga
      601 ttctgattgc tcgcgaatac caaatgcttc cggtggcatc gatacacctg tgtttccagc
      661 aaattggtac ccgagtgtcg tagcataggt atgaaagtta ataacatctg cagggaatgt
      721 tggcgcgcac ggtcgattgt gttcgaacca aaactccatt gcctttaagt atcgcggatc
      781 gtgctgagac actgccggta gtaccacctt catccctggt gctgcatttt ttaccccaac
      841 agtttctcca cggtagcgaa gggtgttgca gtgcccatca taatcggcac ttaccatcgc
      901 tgctgtttca aatggtaagt agtacccagc attttgccaa aacccatcag gctcgttccc
      961 gagttcaatc attcgcaccc ctaaatcgta gtcgcgcttg gcaacccgct ttgagagcca
     1021 tgctttatgt gcgtaacttg ctgggtcttg cgcaaccgta tcacaactcg gcaacccgcc
     1081 aacaatttta ctgcccgcgc agagcgtgta atcaatcgcc ttccgatctg aaaatgacgt
     1141 gtacgcctcg ctgatgtaga gcgggtcagt cgggtcaacc attgtccagg cggcacccat
     1201 cagcacacta tatactccta cccccttctg caccattcga gtgatcgttt caagtgcaat
     1261 cccatcgagc ggagtgagat tatcattttt atcgaatgtc catcgaagtc cgccaaagta
     1321 gcgaactgtc ccccactggt catcatactt tgtgccgttt gcatcttgcc ccattccaaa
     1381 gctaatgttt gtaccaatca ttttttcgaa cgtatagttt ggctttattg tcggaaccgc
     1441 cgccgtgctc ggtgtagacc ccattggcac cccgtagagc accacttcgc cgatgaaacc
     1501 gtgcccatca ttaaagcgca gttgcaagta ccgcgtttcg gttggtgtaa ccgagagccg
     1561 tacccactcg ttctcttttt caaaattatg cgtcgcaatt ttttgggtgt attttagcgg
     1621 tccgcctgtc cacacctgga gcggttcagt atcccccgct gcatgcgtgc caccacggtc
     1681 atcgtagaga tagatgtcag taattttgta ggtgcgacca aggtcaacga ttattttccc
     1741 ggggtacgcc agctctttat tcgagtttgt atcattgacg tgaaagcagg tgcgatccac
     1801 cggcggttct tcccatactc ccttgcacgc cgctggtcgt gttgtttgca gcggattgcc
     1861 acttgcatca agcaccagcg taccttgctc attaaaaagt ttttcttcat tccccactaa
     1921 tgtttcgttg aagagcattt ccggagttat cacgatgcgc cgagcagcag ggtccatcag
     1981 tgttaccgca gttgtcaccg ctatctgcac ctcatcagtt gcagttccgc cgctactatc
     2041 tgttaccgtc agccgaaata cgtacgctcc ctccacaagt cctgttacct gcgtacttgc
     2101 tgttgtagca cttacaatag tagcggctcc gccagaaact tttgtccaca cgtaacttgt
     2161 gatgatgcca tcagcatcag caccacttcc tgaaagagta accgtactcg tcggcagcgt
     2221 tatagcctgg tcacttcctg cacttgctgt cggcgcctga ttccctggaa caggagtagg
     2281 ggttggagct gttcctctcg tttgcgccaa gagccactcg taggtattcg gtgtttgata
     2341 gctgttggtt ggatcatatg tctgagtcca cacgccatgc cccaaccccg gatacaccgt
     2401 cgtttttgtc ggagcagctg atggcgtaca agcagcaagt gcggcgagcg caccgttcac
     2461 tgtccaaatc cacccatcgg catctcccca aatgaaccac gtttttacat tgtttgccgg
     2521 cacaaaacac gcatcaagcg gtgccatgcc gcccgggtag tcgggggtgt aaatactcgt
     2581 tcccgcatca gcaccatcag ttgcaacaac agctgcaatt ttttgagcaa acgccggtga
     2641 aaattttggg tagcggtgcg caccaaatgc accgagcgaa agaccggtga gataaattct
     2701 atctgtatca attcgatagt tttcttttgc gtattcaata aatgcatcaa cctggtacgg
     2761 gtgccactcg ctcgagcctg catcgagctg tggcgcgatt accacaaacg aatcgagttt
     2821 gccgtttacg gtgtactcga gctgcgcgcc ttcattgatc acctttggca aaccggctcc
     2881 aagaacacgc gaaagctcag tcgtaccatt gccacgctcg ccgtccccat gcaaaaaaat
     2941 aatcagcggc cactcgcgag ctgggtcatc attgtagccc tttggtagag actcatagaa
     3001 ccccatctgc tggttgtagt aatttataat agtgatcggc gcccgcgcag tgttcgtgcg
     3061 cactcccggg ttcgcagtac tcgcttcttt aggcgacaaa aagaagccaa cactcgaaat
     3121 accgagaaac agaaaaagaa ccaacaattt tttcatttat atagttttct ctgagagatg
     3181 attaaggctg gtaaagcctt cagtgcgttg tgttaggttc attgtaatac ctttacgcct
     3241 gcatcacaag gaatatagat gttgaaaact gtcttgaaaa taaaaaacaa actaaagatt
     3301 ttggctaatt ttttgtggtg tttttcttgg gtgcacccat gcgggcacac gccaaaaata
     3361 tagtcgcttc gcttcttatt tttgggtgcc tatcgggatt tgaacccgaa ctgagagttc
     3421 cacaaactct agtgctaacc attacactat aggcaccatg tattgctcgc ccaagttggc
     3481 gagcgctctt gtttatacca gatttgtacg cgtaaagtct agcggtttac tgcctctgga
     3541 agcggactgg gttctttgcg aacagagaac cgctcgaata cttgtgaacc aatagttgaa
     3601 acaaccagga gtccgaaata gataaagagt ccgtgctcaa actcaacttt aatggtgcgt
     3661 agcgagccga gaaaaaatgc cgtgtaccac gagacaattg aaacaccccc gagtgcaaat
     3721 gcaagacggc gcagatgggt tggtgctttt gcacgatcaa acgagagctg ggtgaggtac
     3781 ggcgcgactt taaaattcag caaaaaacca tttacaagca ccaccccaac caccactgct
     3841 ttgagtaaaa attttggcga ctcattcagg cgcgctgatt caggaagata gagcgcaaga
     3901 ccagagaata ccaagagcgt gagcccgacc cagataacac tggaaagtgt gtcgagtgtc
     3961 cccttctcgt cttctgaaat gtggtgatct tttaaaaacc gaaagaagag aatgtcggta
     4021 atagttgcgg cgccgagccc gagaataaaa ccaatgttgt gcataataag cgccgcaagc
     4081 cgatattgcc cgagcagtgc aactaccgga gcgagaggga cagtaaaaaa tataagtgta
     4141 aacagtatgg tgttacagag cgccgaagca atgcaccagg tgcaccacga ttttaaaata
     4201 aatgcctgca ccgcggtgag gtagagagaa aagaaaaacg cacctgcagt aaccagcacc
     4261 aaaaagagat cgtagagcgg cgtggcaatt gccggaacaa atagttttgc agcatagagc
     4321 gcccacacga gcaaatagta gcccatgccg agcacctcat tgcgggcacc gagcgttttt
     4381 gagaacctgc tc
//
LOCUS       DATIBQ010000078         5879 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U16294
            k141_1985161, whole genome shotgun sequence.
ACCESSION   DATIBQ010000078 DATIBQ010000000
VERSION     DATIBQ010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935789
            Sequence Read Archive: SRR25158331
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 5879)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5879)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:58:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 637
            CDSs (total)                      :: 603
            Genes (coding)                    :: 601
            CDSs (with protein)               :: 601
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5879
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1985161"
                     /isolate="SMAG_U16294"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..259)
                     /locus_tag="VLB02_02570"
     CDS             complement(<1..259)
                     /locus_tag="VLB02_02570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56943.1"
                     /translation="MNNHLPDPLEQNIEETKKPHKNSNIFLGIFIGIALLTTISLSIY
                     ARNTYGLLASVTMPDIAAINDSNQGSGRLSELSTAVLRTLKG"
     gene            897..1235
                     /locus_tag="VLB02_02575"
     CDS             897..1235
                     /locus_tag="VLB02_02575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56944.1"
                     /translation="MTTTPSIDFDTMRSNCIQIHQECFELSLVTKKLNIPVQTIPHDE
                     LSTNEKTKTWNDMGRYAKELFDNDKMWATLPEKLDIELRTGTKTWSKNEAKKSLLDCY
                     KNLGGELLSV"
     gene            complement(1497..1568)
                     /locus_tag="VLB02_02580"
     tRNA            complement(1497..1568)
                     /locus_tag="VLB02_02580"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1533..1535),aa:Arg,seq:gcg)
     gene            complement(1586..2056)
                     /locus_tag="VLB02_02585"
     CDS             complement(1586..2056)
                     /locus_tag="VLB02_02585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56945.1"
                     /translation="MAETEAPTLSWSVHEFEKKDRHPDWIWAAGFVALVIAVLSFFYG
                     NIFFGIFALIAGGAVIFFALREPKTITVTLSAETVRVDEQIIPYTKIEKFWLDEHGKT
                     DKLLLAAKTGFVPVITIPLGEVAAETVREYLKQRCTESAIPVSFSTELFDRLGF"
     gene            complement(2083..2433)
                     /gene="mscL"
                     /locus_tag="VLB02_02590"
     CDS             complement(2083..2433)
                     /gene="mscL"
                     /locus_tag="VLB02_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007550295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HSE56946.1"
                     /translation="MEQEQKAPASAKKLTFVQKFILFIRTQGVIGLAVAVVLGGAVQK
                     VVTALVNDILNPIIGLFLGKSADLKDAAVTIGNAKILWGDFVSTIIDFLIIALVIYLI
                     IKVLNAGMLDKQKQ"
     gene            2493..3077
                     /locus_tag="VLB02_02595"
     CDS             2493..3077
                     /locus_tag="VLB02_02595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56947.1"
                     /translation="MEEKMNILKLKQYGADSFPFLTEEERLGMLSEAVVAEFQKAPAV
                     IGRVQQNFFEAEVNPYWAPITMRAFSRLEQCIRDTLAAQLKRTLGRFFFNEHRFHRYP
                     VGPVGISGHRDEAKFQLFIAVFVLAGKGDFRTHVDREGKKPLYIYEQPGHCILIPTLG
                     FFDDDNSIPFHSVVDISEERTVVAARFDSLKSGL"
     gene            complement(3060..3422)
                     /locus_tag="VLB02_02600"
     CDS             complement(3060..3422)
                     /locus_tag="VLB02_02600"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HSE56948.1"
                     /translation="MENTIFVRNLTFFGIHGKEAVQRNQKREFHIEATVWIKSIEAAV
                     KSDRLRDAFDYKHIIEAATAVIEGESKNLIESILTEIAERILAHPQAEKVKLTLWKRE
                     LLGSAESGITIVRSKSTL"
     gene            complement(3494..3580)
                     /locus_tag="VLB02_02605"
     tRNA            complement(3494..3580)
                     /locus_tag="VLB02_02605"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3544..3546),aa:Ser,seq:tga)
     gene            3654..4043
                     /gene="ruvX"
                     /locus_tag="VLB02_02610"
     CDS             3654..4043
                     /gene="ruvX"
                     /locus_tag="VLB02_02610"
                     /inference="COORDINATES: protein motif:HMM:NF015607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HSE56949.1"
                     /translation="MKFLGVDYGEKRVGIAISDEAGEFAFPRAVVSRQKAVQEIAALA
                     AEEQVAGIIIGDSVASNEEANQILEKVKQFAAALEKVVPLPIKFEREDYSSVEAHRYQ
                     TKAGAKDDSAAAIILQRFLDKKRGIYS"
     gene            4046..4381
                     /locus_tag="VLB02_02615"
     CDS             4046..4381
                     /locus_tag="VLB02_02615"
                     /inference="COORDINATES: protein motif:HMM:NF013734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56950.1"
                     /translation="METITIDDFKKIEARVGKVLVAEAVPDSDKLIRFELDFGGEQPR
                     QILSAIREWYPEPEKLIGKMLLFVINLEPRTIRGLESNGMLMAVDGSDGAPVFLIPEK
                     EVVPGARVR"
     gene            4417..5496
                     /gene="pilM"
                     /locus_tag="VLB02_02620"
     CDS             4417..5496
                     /gene="pilM"
                     /locus_tag="VLB02_02620"
                     /inference="COORDINATES: protein motif:HMM:NF022554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HSE56951.1"
                     /translation="MRHFFYNRNFPTPRFIALSSVGLDFSDRTLRFVELQASRKGLDL
                     RRFGEEQIPEGLVKGRRIVDTERFSKFLIDVRKKHKLKYVRVSLPEEHVYSFTIAIAA
                     VEPKEVRGAIELVLEDNIPIKAAEAVFDFAILKHDGSHLVVHVVAVLAAIVDSYYRTF
                     EMAGLVPVSFELEAGAIARSVVPRDALTSYMVVDFGALRTGISIVAHGMPLYVATLEV
                     GGAILTQMIAKEQSVPFEEAESMKRAYSIASPEENKQLFSSLINGLSTLKDEINRRYI
                     YWHTKKDGEVEFPNIEAVLLCGGVANLKGLPDYLATNLKLPVRIANPWVNMFSFESRV
                     PELPLEAAMSYATAIGLALGDYDYD"
     gene            5489..>5879
                     /locus_tag="VLB02_02625"
     CDS             5489..>5879
                     /locus_tag="VLB02_02625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56952.1"
                     /translation="MINLLPYNQKRALRRVLKLRVANATLVFFFFLVAVGLVLIIPSF
                     ISVNLRHRLASEQYAAIAESGSFASDEDLAATAAAIALLKSAYAADSTFSVLAALDAV
                     VPPPGITITNISMASREGRSIEFSGISR"
BASE COUNT         1599 a         1246 c         1390 g         1644 t
ORIGIN      
        1 agcccttaag tgttcgaagc actgcagtcg agagttcaga aaggcgaccg cttccttggt
       61 ttgagtcatt aatagcggca atatctggca tagtaaccga ggcgagcagt ccataggtat
      121 ttctggcgta tatgctcagt gaaatagtgg taagaagagc aataccaata aaaatgccca
      181 aaaaaatatt gctgtttttg tggggctttt tcgtctcttc aatattttgt tcgagaggat
      241 cgggtaaatg gttgttcata aagtaacgat aaggctatta aaatagttca gggtatagag
      301 atactctgta cagtagtagt gtagcagtaa tactgcgggt aactcattgt gtggttgtgg
      361 agagagatga gatattttga caattgctgc ccaatagcgt atttttgaat caaaataatt
      421 gttatgaaaa aaattttact tttagtattt ttttgtgcag ttttaatttt ttcttattgg
      481 tttacccatc ggtaatagtc ctttatctag tcctttatct ttattatgaa agatcgagac
      541 tcgcctctac gctgaggagg agttcgaaaa gcgctgattc taggaagtta gcgctttttg
      601 ctttttaact tgttcacgta gcgtcagaaa attggaacca gcttcattcc tggattttcg
      661 aggtctcaga ggtgattata cgaacgaaag agattggaaa gagacttttt tactagatga
      721 agacaaggaa atagtggcca caagggagga atagaacaag taagaagggg atttggtagg
      781 tattgacaat tttatattaa tatgatatca tgttaatata ttagctcctt caaaacgttt
      841 tagtgtttta cagaaatcct tgttgggggt ttgcgttaaa cactaaaact taaaatatga
      901 caacgactcc ttctattgat tttgacacca tgcgttcaaa ttgtattcaa attcaccagg
      961 aatgtttcga gctttcactg gtcacaaaga aactaaatat acccgttcag accattccac
     1021 atgacgagct ttcaacaaat gaaaaaacaa aaacatggaa tgatatggga aggtatgcaa
     1081 aggaactatt cgacaatgac aaaatgtggg ctacattacc agaaaaattg gatattgaat
     1141 tgagaactgg cacgaaaacg tggagtaaaa atgaagccaa aaagtccctt cttgattgct
     1201 ataaaaattt aggaggagaa ttactttcgg tatagaaaat ttctatatcc atcaagcaga
     1261 catcttaacg aggtgtctgt tttttatttg aaaaaattgt cctctcggta gggcgcttcg
     1321 ctatctcact cggaactaac gttcctcttt gagaacccac ggttctcaac gggcgaaacg
     1381 ccctgctctc ctccacggaa aacttccgtt ttccgaccct ttcctggttc gattcctacc
     1441 ttccaagagt catataacaa aactgcccac caaacaggtg agcaattttg ttattgtcct
     1501 ctcggtagga atcgaaccta catcacaact tccgcaaagt tgcgtcctat ccattgaacg
     1561 acgagaggat agtaattatc tcaacctaaa acccaagccg gtcgaagagt tcagtactaa
     1621 acgaaaccgg aatggcagat tcagtacagc gctgctttaa gtactcgcgg acagtctcgg
     1681 ctgcaacctc gccgagcggg atagtaatga ccggtacaaa gcctgttttc gcagcaagta
     1741 acagcttatc tgttttgccg tgctcgtcaa gccagaactt ctcgattttt gtatagggaa
     1801 taatttgctc gtctacccgc actgtttcag ccgagagggt aacggtaatt gtttttggtt
     1861 cccgtagggc aaaaaagatg accgctccgc ccgcaatgag ggcgaatatg ccaaaaaata
     1921 tgttcccata aaagaaggaa agtaccgcaa taaccagcgc aacaaaccct gccgcccaaa
     1981 tccaatcggg gtggcggtct tttttttcaa actcgtgcac gctccacgag agggttggtg
     2041 cttcagtttc agccataggg ctagtatagc aagaaactaa gactactgtt tctgcttgtc
     2101 gagcatgccg gcatttagca cttttataat caggtagata acgagagcaa tgatgaggaa
     2161 atcgataatg gtactgacaa aatcgcccca gaggattttt gcattaccaa tagtaacagc
     2221 tgcatctttt aggtctgcgc ttttgccgag aaaaagccca ataattggat ttagaatgtc
     2281 gtttaccagt gctgtcacta ctttttgcac tgcgccaccg agcactactg ccactgcaag
     2341 cccaatcact ccctgggtcc gaataaagag aataaatttt tgtacaaagg tgagtttctt
     2401 tgccgaggca ggtgcttttt gttcttgttc cataggggtg tagtatactc ccaacagaag
     2461 catttttact taacaattaa aaaccccaag ctatggaaga aaaaatgaac atactgaaac
     2521 ttaagcagta tggagcagat tcgttcccct ttttaaccga agaggaaaga ttaggtatgc
     2581 tttcagaggc tgttgtggct gaatttcaaa aagcaccagc ggtaattgga cgggtacagc
     2641 aaaacttttt tgaagcagag gtcaacccct attgggcacc tataaccatg agggcattct
     2701 ctcgacttga gcagtgcatc agagacacgc ttgctgccca actcaagcgt actctcggta
     2761 ggtttttctt caatgaacat cgctttcatc ggtatccagt tggaccagtt gggattagtg
     2821 ggcaccgcga tgaagctaag tttcaattat tcattgcagt attcgtgctt gcaggaaagg
     2881 gtgatttccg gacacatgta gatcgtgaag ggaaaaagcc actatacatc tacgagcagc
     2941 cagggcactg tattcttatc ccaacactag gattcttcga cgatgataat tctatcccgt
     3001 tccattctgt agttgatatt tctgaagagc gaacggttgt tgctgcccgc tttgattcat
     3061 taaagagtgg actttgatcg aacaattgtg atacccgatt cagcactgcc taacaactcg
     3121 cgcttccaga gcgtgagttt tactttttca gcctgggggt gggcaaggat acgttcagca
     3181 atctctgtta ggatcgactc aatcaaattt ttcgattcgc cttcaataac tgctgttgct
     3241 gcttcaataa tgtgtttata gtcaaatgca tcgcggagtc gatcgctttt gactgcggct
     3301 tcaatacttt ttatccagac cgttgcctcg atatgaaatt ctcgtttttg atttctctgc
     3361 actgcctctt tgccatgtat accaaagaag gtaaggtttc gaacaaatat agtgttttcc
     3421 atagattcat caataaaaag ctagtaagta aaacaggtgc ttttttgtga atcgttaaat
     3481 cattgttttt tagcggaggc ggtgagattc gaactcacgg accagttgcc cagtctcctg
     3541 ttttcaagac aggtgccttc aaccactcag ccacgcctcc gtattctggt gaagtactgg
     3601 gacgatacca taaagatatt cttgtttcaa tacaagcatg gtagagtttt tgtatgaaat
     3661 tcctcggggt tgattacgga gaaaagcggg tgggtattgc catttcagac gaagcaggag
     3721 agtttgcctt ccctagagcg gtggtgagtc gccaaaaggc agtgcaggaa attgccgccc
     3781 ttgcagcgga ggagcaggtg gcaggtatta ttatcggcga ctcagttgcc tcgaatgaag
     3841 aagcaaatca gattttagag aaggtaaagc aatttgctgc agcgctagaa aaagtagtgc
     3901 cattgcctat taaattcgag cgggaagact attcttcagt tgaagcgcac cgataccaaa
     3961 caaaagcggg ggcaaaagat gattcggcgg cagcaatcat tctgcagcga tttcttgata
     4021 aaaagagggg tatatactca taactatgga aacaataaca atcgatgatt ttaagaaaat
     4081 agaagctcga gtggggaagg tgttggtagc tgaagcagta cctgattcgg ataagctcat
     4141 tcggtttgaa ctcgactttg gcggggaaca gccgcgccaa attctttcgg caatccgtga
     4201 gtggtatcct gaaccagaaa agctcatcgg caagatgctc ttgtttgtta tcaatcttga
     4261 accgcgcacc attcgagggt tggaatcaaa cggcatgctc atggcagttg atggcagtga
     4321 tggtgcacca gtatttttaa ttcctgaaaa agaagttgta cctggtgccc gcgttcgata
     4381 gttttttgcg cacgtctctc gtatactaag gagacaatga ggcacttttt ctataatcga
     4441 aattttccga caccgcgatt tatagcgctc tcgtcggttg gactcgattt ctctgatcgt
     4501 acactccgct ttgttgagct gcaggcatca cgcaagggtt tagatttgcg ccgctttgga
     4561 gaagagcaga ttcctgaagg gttggtgaag gggcgacgca ttgttgacac cgagcgtttt
     4621 tcaaaatttt tgattgacgt tcgcaaaaag cacaagctga agtatgttcg agtatcgctc
     4681 cctgaggagc atgtttactc gtttaccatt gccattgctg cggtcgagcc aaaagaggtg
     4741 cgcggcgcga ttgagttggt acttgaagat aatatcccta tcaaggcagc cgaggcagtg
     4801 tttgattttg caatcctcaa gcacgacggt tcgcacttag tggtgcatgt ggtcgccgta
     4861 ttggctgcta ttgttgattc gtactaccgt acgtttgaaa tggcggggct cgttccagta
     4921 tcgtttgaac ttgaagcggg cgcgatcgcc cgatcggtcg ttccgcgtga tgcgctgact
     4981 tcgtacatgg tggtcgattt tggagcactc cgtacaggga tctcaatcgt ggcgcatggg
     5041 atgccccttt acgttgccac tcttgaggtg ggcggtgcga tccttaccca gatgattgcc
     5101 aaagagcaaa gcgttccgtt tgaagaggct gaatcaatga agcgtgcgta cagcatcgct
     5161 tctccagaag agaataaaca gctcttctca agccttatca acgggctctc aacccttaaa
     5221 gacgaaatca accgccgcta catttactgg cacaccaaaa aagacggcga ggtcgaattt
     5281 ccgaatattg aagcagtact cctctgtggc ggggtagcaa acctcaaggg gttgcctgac
     5341 tacctggcaa caaatttaaa attgccggtg cgaattgcga acccgtgggt aaatatgttc
     5401 tcgtttgaat ctcgggtgcc cgagctgccg cttgaagctg cgatgagcta cgcaaccgct
     5461 attgggctcg cattaggcga ttacgactat gattaacctg ctcccgtaca atcaaaaaag
     5521 agcgctccgg cgagtactca agctccgcgt tgccaatgca acactggtgt ttttcttttt
     5581 cttggtggca gtggggcttg tcctgattat cccgtcgttt atttcagtga atctccggca
     5641 tcggctcgcg agcgagcagt atgcagccat tgccgagagc ggatcatttg cgagcgatga
     5701 agatcttgcc gcaactgccg ctgcgattgc gctcttaaag agtgcctacg ctgccgacag
     5761 taccttctcg gtacttgcag cgcttgatgc agtagtgcca ccacccggca ttacaattac
     5821 caacatttct atggcttcac gcgaggggcg gagtattgaa tttagcggta tttctcgcg
//
LOCUS       DATIBQ010000079         7992 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U16294
            k141_871277, whole genome shotgun sequence.
ACCESSION   DATIBQ010000079 DATIBQ010000000
VERSION     DATIBQ010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935789
            Sequence Read Archive: SRR25158331
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 7992)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7992)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:58:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 637
            CDSs (total)                      :: 603
            Genes (coding)                    :: 601
            CDSs (with protein)               :: 601
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7992
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_871277"
                     /isolate="SMAG_U16294"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            435..1670
                     /locus_tag="VLB02_02630"
     CDS             435..1670
                     /locus_tag="VLB02_02630"
                     /inference="COORDINATES: protein motif:HMM:NF014430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HSE56953.1"
                     /translation="MTYQPDVAEQHYVAGNNAIIEAIHAKMGICPYYGTKEILDDILI
                     QKESWGGKDVPENKTKLSAAANVEYMKFAERVGVEKDKPYSSYQELFTQFLVAEKPKL
                     TEKERHIQTLKKLVTLRSAFLDKRLEMEARIKDLAKGLDTLYPIDQAFRAKALEKFRK
                     ENEPLLKEVQEEKDFSWQNLIGYSTAQMGYVQKTILEKEIEHLVVQFPIWEFFGKCIS
                     GFGTWTCAFFIAKLQDPTRFSDSGKVRAFCGMAPKGDQPMRRKRGEKVTYDPDMKEML
                     CYIFPESFMKTCGKFPDEPYVVFFRHCRDKQARKAEQTTVEVLAKKHGVPIERITNLG
                     YKEEEGGRKVFKGFKVKKADGEEITTLNPGHVLQRAMREWGTVFISDFYHAWLFLMGE
                     NLNIGKNPRIMAVFARVKV"
     gene            1913..2317
                     /locus_tag="VLB02_02635"
     CDS             1913..2317
                     /locus_tag="VLB02_02635"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005932620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HSE56954.1"
                     /translation="MEPVNERKQFSVAARAHSLRYALRGIYLFFKSQHNAWVQLVIAI
                     IVVVAGFFFAITSMEWIFVIFSIMAVFSAEAFNTAIEIHMNLTSPNPHPHARDTKDVA
                     AGAVLLTVIGAIIVGFLVFTPYIISFCTYYFQ"
     gene            2502..2849
                     /gene="rpsS"
                     /locus_tag="VLB02_02640"
     CDS             2502..2849
                     /gene="rpsS"
                     /locus_tag="VLB02_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HSE56955.1"
                     /translation="MTRSLAKGPYVDPKLLKKIAGKNPLQSGVIKTWARDSQIAPEMV
                     GFTFGVHNGKVHLEVLVTEDMVGHRLGEFSLTRKFTRHGGAMQKAIDQKAKDAEITAA
                     KAAKAATTDTAKK"
     gene            2874..3242
                     /gene="rplV"
                     /locus_tag="VLB02_02645"
     CDS             2874..3242
                     /gene="rplV"
                     /locus_tag="VLB02_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HSE56956.1"
                     /translation="MKAFLKNYRQSPRKVRLVAGAVKGKRIADAAVALSYMPKRAATP
                     IKKLIESAVANAEAAGSRKEDLVVKSIEVNKGAVLKRWMPRAFGRSSPINKRTSHVQV
                     ELVEKAPKAKKQRSKLTEKK"
     gene            3247..3918
                     /gene="rpsC"
                     /locus_tag="VLB02_02650"
     CDS             3247..3918
                     /gene="rpsC"
                     /locus_tag="VLB02_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HSE56957.1"
                     /translation="MSKVVHPYAHRLVILRDWKSRWFATSKNYVSQLKADVTIREFLE
                     NRLRGSYVSSIEIERGQKLIRLIIRTSRPGMIIGRQGEGATKLRADLLKKIQKEKIVL
                     DGELKIEIVEVSQPEADAKIVSYMIAEGLEKRLPFRRVMKQLVEKVMQVRGVQGARFV
                     IGGRLGGAEISRSEQIKRGSIPLQTFRADVDFARERAHLPYGDIGIKVWIYKGQIFNK
                     DEKQQ"
     gene            3924..4340
                     /gene="rplP"
                     /locus_tag="VLB02_02655"
     CDS             3924..4340
                     /gene="rplP"
                     /locus_tag="VLB02_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006021063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HSE56958.1"
                     /translation="MLVPKKVKFRKWHKMRRNDAKPKVETRGATLAFGSFGLKAESAE
                     RITSNQLEAARKAMSRKLTKVGRVWTRIFPDMPFTRKAAEVPMGQGKGDLAGYCTQVY
                     PGRIIFEVDGVTADVAKEALRKAGTKLPIKTRIIAR"
     gene            4346..4546
                     /gene="rpmC"
                     /locus_tag="VLB02_02660"
     CDS             4346..4546
                     /gene="rpmC"
                     /locus_tag="VLB02_02660"
                     /inference="COORDINATES: protein motif:HMM:TIGR00012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HSE56959.1"
                     /translation="MKKKLDTATKNTGELQTEVATLRAKFRGLAFKAAGAQATSASER
                     RQIKRDIARLLTALRAQVVDKA"
     gene            4575..4817
                     /gene="rpsQ"
                     /locus_tag="VLB02_02665"
     CDS             4575..4817
                     /gene="rpsQ"
                     /locus_tag="VLB02_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012369018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HSE56960.1"
                     /translation="MTNTTGHKKILSGTVVSDKMDKTIVVSVTRFVKHPKYKKYMKIT
                     KKYKAHDEGNTATVGATVRIQEVRPLSKDKHFTLVK"
     gene            4822..5193
                     /gene="rplN"
                     /locus_tag="VLB02_02670"
     CDS             4822..5193
                     /gene="rplN"
                     /locus_tag="VLB02_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020251476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HSE56961.1"
                     /translation="MLQDGSMVKITDNAGGKIGKIFKVLGGSRRRYAGIGDIVVISVK
                     TAEPRKQVKKKDVCYAVIVRQRTAFRRKDGSYIRFDDNAVVIIEKGKQEPKAGRIFGP
                     IPRELAERGYQKIISLAPEVL"
     gene            5200..5505
                     /gene="rplX"
                     /locus_tag="VLB02_02675"
     CDS             5200..5505
                     /gene="rplX"
                     /locus_tag="VLB02_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003664549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HSE56962.1"
                     /translation="MKMKKGDKVIVITGKQKGEKGTITKVLGESNRVVVEGIAKAKVH
                     QKARRSTESGSIIERERSVHTSNVMLVDPKSGSRTRVGKKKVGEKWQRFAKKSGQEL"
     gene            5522..6052
                     /gene="rplE"
                     /locus_tag="VLB02_02680"
     CDS             5522..6052
                     /gene="rplE"
                     /locus_tag="VLB02_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HSE56963.1"
                     /translation="MQLTKDKEAAAFKTIGGYTNKLASPRLTKVVLSVGTGSGMKKSP
                     DRNKFVLERLAKIAGQKPTVRAAKQSIASFKIRQGDPVGLAVTLRGARMYHFLDKLIH
                     VAIPRTKDFRGIDTKVVDSMGNMTLGIKEHTIFPETADEDLRDVFSLAVTLVSTAKTK
                     AEAIAFFTHLGIPFKK"
     gene            6183..6368
                     /locus_tag="VLB02_02685"
     CDS             6183..6368
                     /locus_tag="VLB02_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009984979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HSE56964.1"
                     /translation="MAKTSVIVRSQRKPKYSTRATNRCFRCGRGRGFMRDFGICRICF
                     RELANEGQIPGIRKSSW"
     gene            6373..6768
                     /gene="rpsH"
                     /locus_tag="VLB02_02690"
     CDS             6373..6768
                     /gene="rpsH"
                     /locus_tag="VLB02_02690"
                     /inference="COORDINATES: protein motif:HMM:NF001109.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HSE56965.1"
                     /translation="MVTDPIANMLVSIKNASMVSKESVTIPFSKIKFAIAECLKGSGF
                     VANVSKKTEKENMPVLVIELAYTDAGAKVHDIARISKPSRRTYAGTKDLRPFKSGHGM
                     YVLSTPKGILSDKQAKKEQVGGELLFSIW"
     gene            6773..7306
                     /gene="rplF"
                     /locus_tag="VLB02_02695"
     CDS             6773..7306
                     /gene="rplF"
                     /locus_tag="VLB02_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HSE56966.1"
                     /translation="MSRVGKQQLEIPAAVTVTLENGLFTAKGPLGTLSRQFRPEVAIT
                     IENNIISFTPVALDPLSRALWGTYASHVENMIAGVQTNYVKKLILEGVGFKSEVKGKD
                     LNLALGFSHPVVVPIPEGLVVTAEKNNITVTGIDKELVGQFTAKVRALKKPEPYKGKG
                     FRYDTEVIRRKAGKKTA"
     gene            7313..7660
                     /gene="rplR"
                     /locus_tag="VLB02_02700"
     CDS             7313..7660
                     /gene="rplR"
                     /locus_tag="VLB02_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006784446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HSE56967.1"
                     /translation="MAQQTTQDKKLRIKRKIRSRVSGTAARPRLSVYRSNRFIYAQLI
                     DDSTATTLASATDLKLGKMKKMEAAQAVGAAIAALAKEKKITAAVFDRNGFKYAGRIS
                     ALADAARANGLLF"
     gene            7670..>7992
                     /locus_tag="VLB02_02705"
     CDS             7670..>7992
                     /locus_tag="VLB02_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HSE56968.1"
                     /translation="MNERTPSRKPAGGQRKGGERRPFTDRPKEFDQKMLEVRRVTRVV
                     AGGRRMSFAVSMIIGDRNGMVGVGTGKGNDTSLAIAKALKTARKNAIKIKFTEKRSIP
                     HEVHA"
BASE COUNT         2412 a         1689 c         1945 g         1946 t
ORIGIN      
        1 cggtacaaag cgtccaaaga cgatgtgggg caagattacc ggcggccgaa agacccgtac
       61 gccaaagaag tactcgaacc agctcatcat ctctcgccgc aaaactggca agaaagggaa
      121 ccagtcataa tactctccaa aatcacttcg ctgttcggcg aggtgttttt gactttttta
      181 ttaaacagat gtagggtagt cgagaattaa gcaatgcacg cagtatgtag aacaaaaatc
      241 tattgttttt aagcaaatga gctcctgtgc aaaacaaatg ggttggtgct tttaaacaag
      301 taggagatat gtaaaaaaac aagaatgcag gtgtaccctg ttctcaactg agaacaggtt
      361 tttttgacaa aaaaataaat ttatggtaat aaaaataaga atcgctattt gtaaaacaca
      421 aaaaataaac tactatgact taccaaccag atgttgctga acagcactat gttgcaggta
      481 ataacgctat tattgaagca attcatgcaa aaatgggtat ttgcccatac tacggtacga
      541 aggaaatttt agacgatatt cttattcaaa aagaatcatg gggaggaaaa gatgtccctg
      601 aaaataaaac aaagctttct gctgcggcga atgtcgagta tatgaagttt gcagagcgag
      661 taggtgtaga gaaggataaa ccatattcct cttatcaaga gttatttacc caatttcttg
      721 tcgcagagaa accaaagctt acagaaaaag aacgtcacat tcagaccttg aaaaagttag
      781 ttacccttcg aagtgctttt cttgataagc gcctggagat ggaagcacgc attaaggatc
      841 ttgcgaaagg tttggatacc ctgtatccga tcgatcaggc atttcgcgcc aaagcgctcg
      901 aaaaattccg taaagaaaat gagccgcttc tgaaagaagt acaggaagag aaagattttt
      961 cttggcaaaa cctgattggt tacagtactg ctcagatggg ctacgtgcaa aaaacaatcc
     1021 ttgaaaaaga aattgaacac ctagtagttc agtttcccat ttgggaattt tttggaaaat
     1081 gcatttccgg gtttggtact tggacctgcg cattttttat tgcaaaactt caggatccaa
     1141 cccgtttttc tgattcagga aaagtgcgag cgttttgtgg catggcacca aaaggagacc
     1201 agccgatgcg ccggaaacgc ggtgaaaaag ttacttatga tcccgatatg aaagaaatgc
     1261 tttgttatat ttttcctgaa agttttatga aaacttgtgg aaagtttcct gacgaaccat
     1321 acgttgtttt tttccgccac tgtcgcgata aacaggcgcg caaagctgaa caaacaacag
     1381 ttgaagtgct tgcaaaaaag catggtgtcc cgattgaacg gataacaaat cttggctaca
     1441 aagaggaaga aggcggcagg aaggtgttta aaggatttaa agttaagaaa gcggatggcg
     1501 aagaaataac cactttaaat cctggtcacg tacttcagcg tgcaatgcgt gagtggggaa
     1561 cagtatttat ttctgatttt taccacgcct ggctgttttt gatgggagaa aatctcaata
     1621 ttggtaaaaa tcctcggatt atggcagtat ttgcaagagt aaaagtttag acaaaaacca
     1681 tgtgtgtacg aaacaaatgg gtgcatgtct tgaaacaagt gaacctatgt gtaaaacaac
     1741 tttactaatg ttttaagacc actgctatga aactagagac agggaatgac ttgtgcaaaa
     1801 caaatatttt gttgtcttct atttttttaa ggcaatatta gtcctgttac aaacaaggtt
     1861 gaagatgcca agccctctct actagagagg gcttttgtta tacttccact ttatggaacc
     1921 agtaaatgag cggaagcagt tttcggtggc ggcgcgggcg catagtttgc ggtatgcact
     1981 acgcgggata tacctctttt ttaagagcca gcataacgcc tgggtgcagc tcgttattgc
     2041 aattatagtt gttgtggcag ggtttttctt tgcaattact agtatggaat ggatttttgt
     2101 tattttctca atcatggcgg tattttcagc tgaagcattc aatactgcga ttgaaataca
     2161 catgaattta acctctccaa acccgcaccc gcacgcccga gacaccaaag atgtagcagc
     2221 aggggcagta cttttaacgg ttattggtgc aattattgtt ggttttcttg ttttcactcc
     2281 ttacatcatt agtttttgta cctattactt tcagtagtac caaaaagagt gttttcagga
     2341 cacgcatatt cgcctcgtgg cgaattgctc cgctcgcctt acgccggctg cgcgagctcc
     2401 cgaaaacact ctttttggtt taaggaatat tcgtttgaca tatcagctca atttgttaag
     2461 atagcgctta cggctcggtt agggctattt tatttgttta tatgaccaga tcacttgcaa
     2521 agggtccgta cgtcgaccca aaactcctca aaaagatcgc aggaaaaaac ccactccaaa
     2581 gtggggtaat taagacgtgg gcgcgcgatt cacagattgc gcctgaaatg gtcggcttta
     2641 ccttcggcgt acataacggt aaggtgcacc tagaagtgct cgtaaccgaa gatatggttg
     2701 ggcaccgctt gggagaattt tcgctcaccc gcaagttcac tcgtcatggt ggtgcaatgc
     2761 aaaaggcgat cgaccaaaag gcaaaagatg ctgaaattac tgcggcaaaa gcagcgaagg
     2821 cggcaactac tgacactgct aaaaaataat ttccctaacc ttattacttt tttatgaaag
     2881 catttctcaa aaactaccga caatctcccc gcaaggttcg cctggtagcg ggcgcagtaa
     2941 aagggaagcg tattgccgat gcagctgttg cactctcgta tatgccaaag cgggcagcaa
     3001 cgccaatcaa aaagctgatt gaatcggcag tggcaaatgc tgaagcggct ggttctcgaa
     3061 aagaggactt ggtggttaag tcgattgagg tgaacaaggg ggcggtgctc aagcgctgga
     3121 tgccgcgtgc gttcggacgt tcatcgccta tcaacaaacg caccagtcat gtgcaagtag
     3181 agcttgttga aaaagcacca aaagcaaaaa agcagcgttc aaagctaact gaaaagaaat
     3241 aattatatgt caaaagttgt tcacccctat gcacaccgtt tagtaatcct ccgtgattgg
     3301 aaatcacgct ggtttgctac gagtaaaaat tacgtgagcc aactgaaggc tgatgtaacc
     3361 attcgcgaat ttcttgagaa tcgcctgcgc ggttcatatg tctcttcaat tgaaattgag
     3421 cgtgggcaga agctcatccg cctgattatc cgtacctcac gaccagggat gattattggt
     3481 cgccaaggag aaggtgcaac caaacttcgt gctgacttgc tgaaaaaaat ccaaaaagaa
     3541 aaaatcgttc tcgatggaga attgaaaatt gaaattgttg aagtgtcgca gcctgaagca
     3601 gatgcgaaga ttgtttcgta catgatcgcg gaagggcttg aaaagcgcct cccctttcgc
     3661 cgggtgatga agcagctcgt tgaaaaggtg atgcaggtac gcggcgtgca aggtgcccga
     3721 tttgtaatcg gtggcagact tggcggtgct gaaatttcac gatctgaaca aattaagcgt
     3781 ggctcaattc cactccaaac cttccgtgct gacgttgatt ttgcccgcga gcgagcacac
     3841 ctgccgtacg gcgatattgg tattaaggta tggatatata aggggcaaat ttttaataag
     3901 gatgagaagc agcagtaatg tatatgttgg taccgaagaa agtaaaattt agaaagtggc
     3961 acaagatgcg ccgcaacgat gcaaagccaa aggttgaaac ccgtggtgct accttggcgt
     4021 ttggttcgtt tggacttaaa gcagagagtg ccgagcgtat tacctcaaac cagctcgagg
     4081 cagcgcgcaa ggcgatgtcg cgcaagctca ccaaggttgg tcgtgtgtgg acccgcatat
     4141 tccctgacat gccttttacc cgaaaagccg ctgaagtacc catggggcaa ggtaagggcg
     4201 atttggctgg ctactgcact caggtgtacc caggacgcat catttttgaa gttgatgggg
     4261 taactgccga tgttgctaaa gaagcactcc gaaaggcggg gacgaagcta ccaataaaga
     4321 cgcgaatcat tgctcgctaa cgtatatgaa gaagaagctc gatactgcca ctaagaatac
     4381 gggagaattg cagactgaag ttgccacact gcgggcaaag ttccgtgggc ttgcgtttaa
     4441 agcggcagga gctcaggcaa cttcagcgag cgaacgccga cagataaagc gcgatattgc
     4501 acgactcctc actgcccttc gcgcacaagt agtagacaag gcgtaaaaaa tgggtattat
     4561 agtgaggctt tcatatgact aacaccaccg gacacaaaaa aatactttca ggaactgttg
     4621 tttcagacaa gatggacaaa accattgtgg tttcggtgac ccgctttgtg aagcatccaa
     4681 agtataaaaa gtacatgaag attaccaaaa agtacaaggc acacgatgaa ggaaacacgg
     4741 caacggtcgg agcaacggtg cgcattcaag aagtgcgccc cctttctaaa gataagcact
     4801 ttacgttggt aaaataattg tatgctacaa gacggatcaa tggtaaaaat tacagataat
     4861 gccggcggaa aaatcggcaa gatctttaaa gtgctcggcg gttcacgccg acgctacgcg
     4921 ggtattggtg atattgtggt tatttcggtg aaaacagcag agccccgcaa gcaggtgaaa
     4981 aagaaggatg tttgttacgc agtgattgtt cgtcaacgga cggcatttcg ccgcaaagat
     5041 ggttcataca ttcgttttga tgataatgcg gtagttatta tcgagaaagg aaagcaggaa
     5101 ccaaaggcag ggcgcatttt tggtccaatt ccgcgcgagc ttgccgagcg cggctaccaa
     5161 aaaattattt ctcttgcccc tgaggtacta taaattttta tgaaaatgaa gaaaggagac
     5221 aaagtaatag tgatcaccgg aaagcagaag ggtgaaaagg gtacgattac caaagtactt
     5281 ggcgaatcaa accgagtggt agtagaaggc attgccaaag caaaggtgca tcaaaaggca
     5341 cgtcgcagta ctgaatcggg ctcaatcatt gagcgcgagc gctcggtgca tacatcaaac
     5401 gtcatgcttg ttgatccaaa aagtggcagc agaactcgcg tgggcaagaa gaaggtcggc
     5461 gagaaatggc agcgctttgc gaaaaagagc ggacaagaac tgtaattttt aatacaacac
     5521 tatgcaacta acaaaagaca aagaagcagc agcatttaaa accattgggg gctacaccaa
     5581 caagttagct tcgcctcgcc ttaccaaagt agtactttcg gttggtaccg gctcgggcat
     5641 gaaaaagtct cctgatcgaa acaagtttgt gctcgagcga ttggcaaaaa ttgctggtca
     5701 aaagccaacc gtgcgtgcgg caaagcagtc aatcgcgagc tttaaaatcc gccagggcga
     5761 cccggtgggg cttgcagtaa cgctccgcgg tgcacgcatg taccactttt tggataaact
     5821 tatccatgtt gctattccgc gcactaagga cttccgcggt attgatacaa aagtagttga
     5881 cagtatggga aacatgacct tgggtattaa agagcacact attttccctg aaactgccga
     5941 tgaagatctc cgcgatgtgt ttagtcttgc cgtaacactc gtttcaaccg caaaaacaaa
     6001 agcagaagca atcgcgttct tcactcacct cggtatccca tttaagaagt agttgaccaa
     6061 tcacactgct tctgctagga tactcgggtt catcggtggc atccgttttt ctctctttgc
     6121 ccctcttgtg cgagagccaa agaaactaaa acgcgatatg cgcgaaggac gccttatttt
     6181 ttatggcaaa aacatcagtc attgtaagat cacaacgaaa gccaaagtac agtacgcgag
     6241 caactaaccg ctgttttcgc tgtggtcgcg gtcgaggttt tatgcgcgat ttcggcatct
     6301 gccgcatctg cttccgtgaa ttagcaaacg aaggtcagat tccgggcatt cgtaagtcat
     6361 cgtggtaatt atatggtaac cgatccaata gcaaacatgc tcgtttctat caagaacgcg
     6421 agtatggtct caaaagaatc agtcacgatc ccgttctcga agatcaagtt cgcgattgct
     6481 gaatgcttga agggcagcgg ctttgtggcg aatgtgtcaa agaaaaccga gaaggagaac
     6541 atgccggtgc tggtaattga gcttgcgtat accgatgccg gtgccaaggt gcacgatatt
     6601 gctcgcattt caaagccgtc tcgccgcacg tacgcaggca ctaaggatct tcgcccgttt
     6661 aagagcggac acggcatgta cgtgctctca acaccaaaag ggattttgag cgataaacag
     6721 gcaaaaaaag agcaggtcgg cggcgaatta ctgttttcaa tctggtaaat ctatgagtcg
     6781 agtcggaaaa caacaacttg aaattcctgc agcagtaacc gtaacacttg aaaacgggct
     6841 cttcaccgca aaaggaccgc tcggcacgct ttcacggcag tttcgccctg aggtagctat
     6901 tactatcgag aacaacatca tttcatttac tcctgttgca ctcgatccgc tctctcgagc
     6961 actctggggt acctatgcat cgcatgtaga gaatatgatc gcaggtgtgc agacaaacta
     7021 cgtaaaaaag cttattcttg aaggggtggg atttaagagt gaagtaaaag gaaaagacct
     7081 caacctcgcg cttggatttt cgcacccggt tgttgttcca atcccagaag ggcttgtggt
     7141 aaccgcagaa aaaaataaca ttactgttac tggtatcgat aaagagctgg tagggcaatt
     7201 tacggcaaag gttcgagcgc tgaaaaagcc agagccgtac aaagggaaag ggttccgtta
     7261 cgatactgaa gtaattcgcc gaaaagccgg taagaagacc gcataaccat atatggcaca
     7321 gcaaacgaca caagataaaa aactccgcat taagcgcaag atccgttccc gtgtttctgg
     7381 aacggcggct cgtccacgcc tttcggtgta tcgctcaaac cgttttatct acgcgcagtt
     7441 gattgacgac agtaccgcta caactctggc aagcgcaacc gacctaaagc ttggtaagat
     7501 gaagaagatg gaagcagcac aagcagtagg cgcagcaatt gctgctttag caaaagagaa
     7561 gaagattacc gcagcagttt ttgaccgaaa cggatttaag tacgcagggc gcatcagtgc
     7621 actcgccgac gcagcgcgcg caaatgggtt actattttaa tactatttta tgaacgaacg
     7681 aacaccatca cgaaaaccag caggagggca aagaaaagga ggagaacgcc gaccgtttac
     7741 tgaccgtcca aaagaatttg atcaaaaaat gctcgaggta cggcgcgtaa cccgcgtggt
     7801 tgccggtggt cggcgtatga gttttgctgt ttcaatgatc attggcgatc gtaacggcat
     7861 ggtgggagta ggaaccggta agggtaacga cacctcgctc gctattgcta aagcacttaa
     7921 aactgcccgt aaaaatgcaa tcaagattaa gttcactgaa aagcgttcga ttccacacga
     7981 agtgcacgca aa
//
LOCUS       DATIBQ010000080         2953 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U16294
            k141_2432866, whole genome shotgun sequence.
ACCESSION   DATIBQ010000080 DATIBQ010000000
VERSION     DATIBQ010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935789
            Sequence Read Archive: SRR25158331
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2953)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2953)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:58:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 637
            CDSs (total)                      :: 603
            Genes (coding)                    :: 601
            CDSs (with protein)               :: 601
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2953
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2432866"
                     /isolate="SMAG_U16294"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(101..1192)
                     /locus_tag="VLB02_02710"
     CDS             complement(101..1192)
                     /locus_tag="VLB02_02710"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HSE56969.1"
                     /translation="MKQKIPLFIFALSLCFAPLITRAQIDILTKEERLWLQNRNNTIV
                     VYPEKNNPPFVYLPSPTAEPIGLSIEYLERIAEKVHARLSFLPAKSRAQVLAEVAEGK
                     GDVVVRTDETVGSEASALRYTQSYLSVPVVLAVRRDSRDSELTLADLAGKRIAVTSAS
                     GAAEFIRKNYPRVVVHQVTDDEIGLQQLVLGEVDAQAIDLASLSYFLSRQALSAVKVV
                     GHTGFYYQLAFAVPEGKAILQSILDKGLTQVSDTERQLISEKWMQASARTLERTPRSW
                     FGGDQTFVIVAAILGGALLISLVLLGIAKWHYRTSRFYKRFQRQDEAKEKLRELEAAS
                     AVIESELKEIKALEHEVASVITDTKTDHL"
     gene            complement(1223..2341)
                     /locus_tag="VLB02_02715"
     CDS             complement(1223..2341)
                     /locus_tag="VLB02_02715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56970.1"
                     /translation="MKRIVAQLVGVILLLVPTLVNASSATLSVSSTKKIVTAGEQFTL
                     TVRAASVDQPINAISGTLVLPVGVVRVGSITTTGSALNFWIREPKVVGNTIPFEGIIL
                     SPGYQGAHAVVFSVTLTATRSGAATFRLSEGAILAHDGKGTNVLGTLSGAMVRITSSD
                     SQPVDQPESETVTYIPSPETPKRLPALPVVTEYSDAVAPGAELFIRGKGEPQAITKLT
                     FQDMAARSLGERFINMLQTKRYRLSDVFIKNDETGNFEYHTPKSLLAGSYNAMPFLVD
                     AESGTEKPGLGAKIFVSDSPMVGFLIVLLNVLALLVPVVGLIVLIYFIPWYSWRRMRV
                     LRRRLGLEEEKLIVSEHQLEREDRLREKITNMPEQLGK"
     gene            complement(2338..>2953)
                     /locus_tag="VLB02_02720"
     CDS             complement(2338..>2953)
                     /locus_tag="VLB02_02720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56971.1"
                     /translation="EQPASTTPGIVTFTGIIPGGATQLYDPLDSKSHELLLTQLLFTA
                     LQPGTPNFSIRSSDIRVHDGKGTPLAYIVSDQVVTTAPSVEVLVPDAIPPAPFAVVHQ
                     SASAVTRTPELIVFAAADAESGIARYEAKIGGSRWKPAQSPLALNYHALPLAVAIRAY
                     DFSGNVQEERVQIPGSGFSGAAAAGGALLLFIIVLLRAMLSKRS"
BASE COUNT          768 a          751 c          702 g          732 t
ORIGIN      
        1 ggaggcggtg atcgtcgccc cttccagcga cgctcgttct agcttgtaga acagtttctt
       61 tcactaaaac actcacgcat gcgcgtgggg gttttaggtt ttatagatgg tcagttttag
      121 tatccgtaat cactgaagca acttcatgct cgagcgcttt aatctccttc agctcagatt
      181 caataacggc actcgcagct tcaagctcgc gcagtttttc ttttgcctca tcctgccgct
      241 gaaatcgttt ataaaaacga gatgtccggt agtgccactt tgcaatacct aagaggacga
      301 gcgaaataag cagtgcgcca cctaaaatag cagcaacaat aacaaatgtc tggtcccccc
      361 caaaccaaga acgcggcgta cgctcaagag tacgagccga agcttgcatc catttttctg
      421 aaatcagctg ccgctcggtg tctgaaacct gcgtgagtcc cttatctaaa attgattgga
      481 ggattgcttt cccctcggga acagcgaatg caagctggta gtaaaagccg gtgtgcccga
      541 ccacctttac tgccgaaagc gcctgccgcg aaagaaagta cgagagcgag gcaagatcaa
      601 tcgcctgagc atcaacttca cccagcacga gctgctgcaa cccaatttcg tcatcggtta
      661 cctgatgcac caccactcga ggataatttt tccgaataaa ctcagcagca cctgaagcac
      721 tcgttacagc aatccgctta cctgccaaat cagctaaggt aagctcagaa tctctgctat
      781 cgcgccgcac tgcaagcact accggcacgc tcaagtacga ctgtgtgtag cgcagcgcag
      841 atgcttcact gcccaccgtc tcatcagtac gcaccacaac atcacctttt ccttctgcta
      901 cctctgctag tacttgggcg cgcgattttg ccggtaaaaa cgaaagccgt gcgtgtactt
      961 tttcggcaat gcgctcgagg tactcaatcg aaagaccgat tggttcggcg gtagggctcg
     1021 ggaggtagac aaacggaggg ttattttttt ctggatatac cacaatggta ttattgcgat
     1081 tttgaagcca aagccgttct tctttggtaa gaatgtcgat ttgtgcacga gtaataagtg
     1141 gtgcaaaaca gagagaaagt gcaaaaataa aaagaggtat tttttgcttc atgtgcatat
     1201 atactagcac aaaatcaccc ctctacttcc ctagctgctc gggcatatta gtaatttttt
     1261 cgcgaagccg atcttcccgt tcaagctgat gttcagagac aatgagtttt tcctcctcaa
     1321 gcccaagccg gcggcgcagc acgcgcatcc gcctccacga gtaccacggg ataaagtaaa
     1381 taaggactat cagcccaacc acgggcacta aaagtgcgag cacatttaag agtacgatca
     1441 ggaagccgac catcgggcta tcagaaacaa atatctttgc acccagcccg ggcttttcag
     1501 tgccgctctc cgcatcaact aaaaacggca ttgcattgta cgaaccagcg agtagcgatt
     1561 ttggggtatg gtattcaaaa tttcctgtct cgtcattttt tataaataca tcgctcaaac
     1621 ggtagcgctt tgtttggagc atatttataa agcgttcacc aagcgaacgt gctgccatat
     1681 cttgaaaggt aagtttagta attgcctgcg gctccccttt tcctcgtata aataactcgg
     1741 ctccaggcgc tactgcatcg gaatactcag taaccaccgg aagtgcaggt aggcgcttcg
     1801 gcgtttcagg tgaagggata tatgttaccg tttcagactc tggctgatcc accggttgag
     1861 aatcagaact agtaattctt accatggcac ctgaaagtgt cccgagtaca ttcgttccct
     1921 taccatcatg cgcaagaatt gcgccctcgc ttaagcgaaa tgttgcagca cctgaacggg
     1981 tagcggtaag tgtaacacta aacactactg catgtgcgcc ttggtaccca ggactgagaa
     2041 taatgccttc aaagggaatg gtattcccta ctacttttgg ctctcgtatc caaaaattaa
     2101 gtgcactgcc tgtagtagtt atcgagccaa cccggacgac accgactggt agtaccagtg
     2161 ttcctgaaat agcattgatc ggctgatcga ctgatgctgc tcgaacggta agagtaaact
     2221 gttccccggc ggtaactatt ttttttgtac tagaaacaga gagtgtagca gatgaagcgt
     2281 tcaccagggt tggaacgagg agcagtataa ccccaactaa ttgtgctact atccgcttca
     2341 tgatctcttt gatagcatag cgcgaagcaa cacgattata aataggagta gtgcgccgcc
     2401 ggctgcagcc gctcctgaga atccagagcc aggtatctgc acgcgttctt cttgtacgtt
     2461 tcctgaaaag tcatacgccc tgatagcaac cgctagcggg agtgcgtgat agttaagcgc
     2521 aagtggactc tgtgccggtt tccatcggct gccgccaatt tttgcttcgt agcgggcaat
     2581 gccgctctcc gcatcagctg cggcaaaaac tatcagctcg ggggtacggg ttactgctga
     2641 tgcagactgg tgtaccactg caaatggtgc cggcggtatc gcatcaggaa caagcacttc
     2701 caccgaagga gcagtggtaa ctacctggtc tgaaacaatg taggcaagcg gcgtcccctt
     2761 gccgtcatgg acacgaatat cagaagaccg tattgaaaag ttcggcgttc ccggctggag
     2821 tgctgtaaag agtaattgtg tgagtaaaag ttcatgactt tttgaatcaa gtgggtcata
     2881 caattgcgtt gcaccgcccg gaataatacc cgtaaaagta actatacccg gagtggtgct
     2941 cgccggctgc tct
//
LOCUS       DATIBQ010000081         5338 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U16294
            k141_203978, whole genome shotgun sequence.
ACCESSION   DATIBQ010000081 DATIBQ010000000
VERSION     DATIBQ010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935789
            Sequence Read Archive: SRR25158331
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 5338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:58:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 637
            CDSs (total)                      :: 603
            Genes (coding)                    :: 601
            CDSs (with protein)               :: 601
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5338
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_203978"
                     /isolate="SMAG_U16294"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1294)
                     /locus_tag="VLB02_02725"
     CDS             complement(<1..1294)
                     /locus_tag="VLB02_02725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56972.1"
                     /translation="MKLKHLESVNNNNCLKKLFAFVLFVVLFFAVSQSAEAASRYWVG
                     GTGTWDQSTTTHWSASSGGSGGASVPTASDDVFIDSSSGSGTITVSATAPMLSLDFTG
                     YTGTFAGSAATTISGSLTLGAGMTLTYTGNITFNATSGSRTITSNGKSLASSVTFNGT
                     GGTWAIQDTFVTTQGIVLVAGTLNLNNFNTTSSSFVGTGTGVRTLTMGSGTWTLSGSG
                     IVWDIENASVTPDITFNANTSTIKVTNTSGAVTFRGGSHTYNKLWFSKGADTNSITIT
                     GSANTFGELKDDGTAAHSLFFGFNSTTTISTWNISGTPGNLITISSSDGSTGVHLLVK
                     VGSYVDADYLNIQHSAASPVGRWFAGTHSVDNQAVATAGSGWAFTVPDTTAPVIAEVT
                     PVSSPTADATPNYTFSSTEAGTISYGGSCSSATTSAASG"
     gene            complement(1263..1394)
                     /locus_tag="VLB02_02730"
     CDS             complement(1263..1394)
                     /locus_tag="VLB02_02730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56973.1"
                     /translation="MFSLRKTLVSFLILTAFSSFLAYKIIDLGYDNMYEAQALGISE"
     gene            complement(1414..2607)
                     /gene="tgt"
                     /locus_tag="VLB02_02735"
     CDS             complement(1414..2607)
                     /gene="tgt"
                     /locus_tag="VLB02_02735"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HSE56974.1"
                     /translation="MQFSINKTLPGTLGRTGTITTPHGTIETPAFVVVGTKATVKALT
                     PEQVRSLGAQVVLANTYHLYLQPGDELVKQAGGFAKFMNWNGPTMTDSGGFQVFSLGE
                     AFGKGGVSKVAKGESVIVPESEHTAHAKLASISEEGVEFSSHIDGSKHFFTPERSMEI
                     QWNLGADIIFAFDECTSPTVPESYQREALDRTYRWAQRSITAHEKLDTENKQALFGVV
                     QGGQVKELREHAARTIGALNFDGFGIGGSFTKEDMYTAVGWVNAILPPEKPRHLLGIG
                     EPEDLFGGVENGCDTFDCVAPTRMARNGTLYTKDGKINMLNAKFVNDFSPVDSTCQCY
                     ICMNYTRAYLAHLFRANEMLAGTLASIHNLHFIVSLVDTIRASIYDDTYCAFKESFLT
                     RFLKK"
     gene            complement(2708..3469)
                     /gene="mreC"
                     /locus_tag="VLB02_02740"
     CDS             complement(2708..3469)
                     /gene="mreC"
                     /locus_tag="VLB02_02740"
                     /inference="COORDINATES: protein motif:HMM:NF016011.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005618 - cell wall [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HSE56975.1"
                     /translation="MRKYGRSRQVRPATVILAAVLLLVTIFFRPLSRGVKTIATPLWQ
                     TRQAANEFTNRLLQSKKSLAAELASVEAELTESRLKIIELSTIEEENKELRKLLGRGE
                     PGEVVLAGILTHPQQSLYNTFQIDAGTGEGIVVGARVFVGTSMLVGTITDVTARGATV
                     TPFASPGNQTSAYVEGSNVSVTITGRGGGDFEVELPRDIPFDPGMAIVSQGIHSYIIA
                     IIEHVISDPRDPFQKLLARMPVNLRTVKWVTVEKK"
     gene            complement(3453..4499)
                     /locus_tag="VLB02_02745"
     CDS             complement(3453..4499)
                     /locus_tag="VLB02_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HSE56976.1"
                     /translation="MFDRLYRRFSNDIGIDLGTANTLVYLKRHGIVISEPSVVAVNQK
                     TGTIVAVGREAKDMQGRTPGHIKTIRPLVEGVVSDFEMTEEMLAYFIRKAEKLSKKFF
                     RPRVVIGVPSGITNVEMRAVYDAAINAGAREVHLVEEAMAAALGVRLPVLEATGSFVV
                     DIGGGTTDIALIALGGIVRSLNLRIAGDHFNTALIEYLRDEFKLLVGEQTAEGIKIAL
                     GSAIETDYMELEVRGRDMVTGLPRSLIITDGDVREALRYPLSLLVVGIREVLETSPPE
                     MLSDIITSGIVLTGGGSLLHGLATLLSAELKLPIYVADDPLTAVARGTGVILEDFKKH
                     QSVLLSLETYAEVR"
     gene            complement(4507..>5338)
                     /locus_tag="VLB02_02750"
     CDS             complement(4507..>5338)
                     /locus_tag="VLB02_02750"
                     /inference="COORDINATES: protein motif:HMM:NF012796.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminoacyl--tRNA ligase-related protein"
                     /protein_id="HSE56977.1"
                     /translation="NETRAKSGIMRTREFVMKDLYSFSKNEVEHLSFYEKVKQAYLNI
                     FKRIGIGDITYVTFASGGVFSKFSHEFQTVTDAGEDIIYIHKEKNIAINKEVFTNDVK
                     KDLVGLDENFVEAKAVEVGNIFTLGTRFSDALGLTYTNEAGESQSVFMGSYGIGPARL
                     MGTVVEVLSDEKGIVWPEAIAPFKVHLLVLGEKPEIRAEAERIYKFLQKEGYEVLFDD
                     REAVLAGEKFSDADLLGMPVRVVVSERALAAGGYEVKRRNSTETTIVTDHDLAALLAL
                     "
BASE COUNT         1407 a         1387 c         1126 g         1418 t
ORIGIN      
        1 tccctgaagc tgccgatgtc gtagcactcg agcatgagcc gccgtaactg atggtacccg
       61 cttcggtgga actgaaggtg tagttggggg tagcatcagc tgttgggctt gaaacaggag
      121 ttacttcagc aatcactgga gctgtggtat caggaacagt aaaagcccag ccgcttccgg
      181 cagttgcaac agcttgatta tcaacactat gcgttcccgc aaaccatctt cctacaggag
      241 aagccgctga atgctggatg ttgagataat cagcatcgac atagctcccc accttgacaa
      301 gtaaatgcac tccggtactc ccatcactac tactgatagt aatcaaatta ccaggagttc
      361 cagaaatatt ccaggtactt attgttgttg tcgaattgaa accaaagaat agtgagtgag
      421 ctgccgtacc atcatctttt aattcaccaa acgtattagc ggagcctgta atggtaattg
      481 aattagtatc agctcctttt gagaaccata atttattata tgtatggcta cctccacgaa
      541 aagtaaccgc acctgacgtg ttggtaactt ttattgttga cgtattggca ttaaatgtaa
      601 tatcaggcgt tacactagca ttttctatat cccacactat tcctgaaccg gaaagcgtcc
      661 atgttcccga acccatggta agtgtcctca ccccagtacc agttccaaca aaagaactac
      721 tagtggtatt aaagttatta agatttaatg tccccgctac gagcactata ccctgtgtag
      781 taacaaaggt atcctgtatt gcccacgtac cacctgtacc gttaaacgta acagaagaag
      841 caagactttt accattactc gttatagtac gtgatccgct tgtggcatta aaagtgatat
      901 ttccggtata ggttaatgtc attcccgcgc caagtgttag ggaaccagaa atagtagtcg
      961 ctgcagaacc agcaaatgtt ccggtgtagc cggtaaaatc aagactgagc attggggcag
     1021 tggcactgac agtgatcgtt ccactgccgg aactgctatc aataaacaca tcatctgacg
     1081 ctgtcggcac cgatgctccc cccgagccgc ctgaagatgc cgaccagtgt gtcgtagtgg
     1141 actgatccca tgttcccgta ccaccaaccc agtagcgact cgctgcttct gcgctttgac
     1201 ttacagcaaa aaagagtaca acaaaaagaa caaaagcaaa gagtttcttt aaacagttat
     1261 tgttattcac tgattccaag tgcttgagct tcatacatat tatcgtaccc aagatcaata
     1321 attttatatg ccagaaacga gctgaaggca gtaaggatca aaaacgaaac gagtgttttc
     1381 cgaagactga acatggctat aagtataaca gatttacttt tttaaaaagc gagtgaggaa
     1441 tgattctttg aaagcacagt atgtgtcatc gtatattgaa gcacggatag tgtcgacgag
     1501 cgaaacaata aaatgaaggt tatggattga ggcaagggtg ccggcgagca tttcgtttgc
     1561 acggaagagg tgcgccaggt acgcgcgagt gtagttcata cagatgtaac actgacatgt
     1621 ggaatcaact ggagaaaaat cgttcacaaa ttttgcattt aacatattta ttttgccgtc
     1681 ttttgtatag agtgtaccgt tgcgcgccat ccgggttggg gcaacacaat caaacgtgtc
     1741 acaaccattc tctaccccac caaacaaatc ttccggctca ccaataccca agaggtgccg
     1801 cggtttttct ggcggaagta ttgcatttac ccaacctacc gctgtataca tatcctcttt
     1861 agtaaacgat ccgccaatac caaacccgtc aaagttgagc gcgccaatcg ttcgcgctgc
     1921 gtgctcccgc aattctttta cttgcccgcc ctggaccaca ccaaagagtg cttgtttatt
     1981 ttcagtatcc aatttttcat gcgctgtaat tgagcgctgt gcccagcggt aggtgcggtc
     2041 gagggcttcg cgctgataac tctcaggtac agttgggctc gtacattcat caaaagcaaa
     2101 aataatatca gctcctaaat tccattgaat ttccatcgat cgctcggggg taaaaaaatg
     2161 ttttgagcca tcgatatgcg agctgaattc aacaccttct tcagaaatgc tcgccagttt
     2221 tgcatgcgcc gtgtgttcac tttctggaac aataaccgat tctccttttg ccaccttact
     2281 caccccgcct ttaccaaacg cttcaccaag tgaaaatacc tgaaagccgc ccgagtcggt
     2341 catggtcggt ccattccagt tcataaattt tgcaaacccg ccggcttgtt ttacgagttc
     2401 atctcctggc tgcaaatata agtggtaggt attggcgagt actacctgcg ccccaagtga
     2461 gcgtacctgc tcgggagtaa gcgcttttac tgttgccttg gtaccaacca ccacaaatgc
     2521 cggcgtttcg atggtgccgt gaggtgttgt tatggtcccc gtgcgaccga gcgtcccggg
     2581 aagtgtttta ttgattgaaa attgcatact tgactatttt aaataaatgt gctaccatca
     2641 agctagcttg tagttggctc ttttgccagt catgagtccc gagccctgcc cgtcaaacgg
     2701 tggggcttta ttttttctcg accgtcaccc acttcaccgt ccgcaagttt accggcatcc
     2761 gcgccagtag tttttggaac ggatcgcgcg ggtcagaaat aacatgctcg ataatcgcaa
     2821 ttatatacga atgtatgccc tggctcacaa tcgccattcc cggatcaaac ggaatatcgc
     2881 gcgggagctc gacctcaaaa tctcccccac cgcgcccggt aattgttact gaaacatttg
     2941 aaccttcaac atacgcactc gtttgattac ccggactcgc aaacggcgtt acagttgcac
     3001 ctcgtgctgt cacatcggta atagtaccaa caagcatgct ggtgcccaca aacactcgag
     3061 ctccaactac aataccttcc ccagtacctg catcaatttg gaaggtgtta tagagcgact
     3121 gctgcgggtg agtgaggata ccagcgagca ccacctcgcc cggctcgcct ctgccgagga
     3181 gttttctcag ctctttgttt tcctcttcga tagtactaag ctcgataatt ttcaagcgcg
     3241 actcggtgag ttctgcttca accgaggcaa gttcagcagc aagggatttt ttactttgca
     3301 aaagtcggtt agtaaattca tttgcagcct gacgcgtctg ccacaatggt gtagcaatag
     3361 tttttactcc ccgagagagc ggtcggaaaa aaatagttac cagcagcaac actgctgcca
     3421 agataactgt tgccggacgt acctgccgcg atctaccgta cttccgcata ggtctcaagc
     3481 gagagtagta cactctggtg ctttttaaaa tcttctaaaa taacgccggt tccccgcgct
     3541 accgcggtga gcggatcatc agccacatag atcggcagct tcagttcagc tgagagcagt
     3601 gttgccagcc catggaggag cgacccgccg ccggtgagca caatgccgct cgtaataata
     3661 tccgagagca tctccggcgg cgatgtttca agtacttcac gaataccaac caccaagagc
     3721 gagagcgggt accggagcgc ctcgcgcaca tcaccatcgg taataataag cgagcgcggg
     3781 agtcctgtta ccatatcgcg accgcgcact tcgagctcca tatagtcagt ttcaatcgcc
     3841 gaaccgagcg cgatttttat tccctcagct gtctgctcgc ccaccagcag tttaaattca
     3901 tctcgcaggt actcaataag cgcagtatta aaatgatccc ctgcgatccg caaattgagc
     3961 gatcgtacaa taccgccgag cgcaatgaga gcgatgtcgg tagtgccacc gccaatatca
     4021 accacaaacg accctgtcgc ttcaagcacc ggcagccgga ccccgagtgc tgctgccatc
     4081 gcctcctcaa ccaaatgcac ttcacgcgcg ccggcgttaa ttgccgcatc atacaccgcc
     4141 cgcatttcaa cattcgtaat ccccgacggc accccaatta ctacccgcgg tcgaaagaat
     4201 ttttttgaga gcttttcagc tttgcggata aagtacgcga gcatttcttc ggtcatttca
     4261 aaatctgaaa ccacaccttc taccaatgga cgaattgttt ttatgtgccc tggcgtacgc
     4321 ccctgcatat cttttgcttc acgcccgacc gctacaatcg tccccgtctt ttgattcact
     4381 gctaccactg acggctcaga aataacaatg ccgtgccgct ttaaatacac cagcgtattc
     4441 gccgtgccaa ggtcgattcc aatatcgttt gaaaatcggc ggtagagtct atcaaacatg
     4501 cattagttag agtgctaaga gtgctgctaa atcgtgatcc gttacaattg ttgtttcggt
     4561 actgtttcga cgcttcacct cgtacccgcc tgccgcaagt gcacgttccg ataccactac
     4621 ccgcaccggc atcccgagca ggtctgcatc agaaaacttt tctcctgcta acaccgcttc
     4681 acggtcatca aagagcacct cgtagccttc tttttgtaaa aacttatata tccgttctgc
     4741 ttcagcacgg atttctggct tctcaccaag tactaacaaa tgtactttga atggcgcaat
     4801 tgcctcaggc cagacgattc ccttttcatc cgagagcacc tcaacgaccg tgcccatgag
     4861 ccgtgctgga ccaatgccgt atgaacccat gaataccgac tgtgattctc ctgcttcatt
     4921 agtgtaggtt aaaccgagtg catcagagaa tcgtgtgcca agggtaaaaa tatttccaac
     4981 ctctactgcc tttgcttcta caaaattttc atctaaccct actaaatctt tttttacatc
     5041 atttgtaaaa acttctttat taatagcaat atttttttct ttgtggatat aaataatatc
     5101 ttcacctgca tcagtcactg tttgaaattc atgagaaaat tttgaaaata cgccccctga
     5161 agcaaaagtt acatatgtta tgtccccaat tcctatacgc ttaaaaatat ttaaatatgc
     5221 ttgttttact ttctcataaa aagacagatg ctctacttca ttttttgaaa atgagtacaa
     5281 atctttcatt acaaattcac gcgtgcgcat gataccgctc tttgcgcgag tttcgttc
//
LOCUS       DATIBQ010000082        13813 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U16294
            k141_1292429, whole genome shotgun sequence.
ACCESSION   DATIBQ010000082 DATIBQ010000000
VERSION     DATIBQ010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935789
            Sequence Read Archive: SRR25158331
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 13813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13813)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:58:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 637
            CDSs (total)                      :: 603
            Genes (coding)                    :: 601
            CDSs (with protein)               :: 601
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13813
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1292429"
                     /isolate="SMAG_U16294"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1410)
                     /locus_tag="VLB02_02755"
     CDS             complement(<1..1410)
                     /locus_tag="VLB02_02755"
                     /inference="COORDINATES: protein motif:HMM:NF012361.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HSE56978.1"
                     /translation="MNNNTNEIKKSAAAEREERVLEFWKEKEIFKKSLEKTKDGKPFV
                     FYEGPPTANGKPGIHHLEARAFKDVIPRYKTMQGFSVRRKGGWDTHGLPVELQVEKEL
                     GLKSKKEIEQYGIAEFNKKCKESVWMYVDLWTRFTDRIGYWVDQKDPYVTYHNNYIES
                     VWNILGKVEKDGLLYKDYKVLPWCPRCGTALSSHELAQGYADVKDLSVYVKFKIKIPT
                     KDVSQSSGRFAQPDMEKIFGGGTAPVYLLAWTTTPWTLPGNAALAVGAAITYSIIEKE
                     NELLILAKERLDGVEGEYTVLGELTGSELVGLEYEPLYPYLSEQINGTEKEKLTNAYK
                     VYAADFVTTNDGTGIVHIAPMYGQDDFELGTQVGLPKLHTVDDSGHFIKGTSFLEGQF
                     VKDESTAVEIIKDLAHRGLLFKKEKYEHSYPHCWRCKTPLIYYARDSWYIRMSDERVK
                     HTLIEENKSINWEPVHIRDG"
     gene            complement(1490..2128)
                     /locus_tag="VLB02_02760"
     CDS             complement(1490..2128)
                     /locus_tag="VLB02_02760"
                     /inference="COORDINATES: protein motif:HMM:NF037785.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56979.1"
                     /translation="MEEVKNNNSNKYVCYNSPLENSEKHGHVKLKFGVSGAAETGHCG
                     LDALEKAKELGRELVRQGGLVLTGATTGFPLWAAMGAKEAHGICIGISPAATAKEHVE
                     TYRLPLDYMDLIIYTGFGFPGRDILFTRSSDAIFIGCGRIGTIHEFTIAFEDGKPVGV
                     LEGPWTMADQIRDMIEKGNRPTDRVIFDADPKRLVQRVIAMVEEDQKKYQSL"
     gene            complement(2133..3500)
                     /locus_tag="VLB02_02765"
     CDS             complement(2133..3500)
                     /locus_tag="VLB02_02765"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HSE56980.1"
                     /translation="MSESKKLKLTFAGGTGSVTGANFLLEGEGKRFLVDCGLAQGTKL
                     ADESNWEAFTYDPTSIDFLFITHAHIDHVGRIPKLIHDGFKGTIYSTIPTKDIAELML
                     EDTANILSRSDNELLGKIYSPDILKKVFAHWQVVDYHQEIRIDPFLFRFRDAGHILGS
                     GMLEIIYNNKKIVFTGDLGNSPSPLLNDTEVLQNVDYLIMESVYGDRNHISRNERRKL
                     LEGVIEDNFKKNGALVIPTFSLERAQELLYEIDSLVEHDRIPRMPVFLDSPLAIKLTR
                     VYKDHTRYFNAEARAAIGEGDDIFNFPGLKLTLLTEESKSILHVPNPKIVIAGSGMSN
                     GGRVLHHEINYLPDESNTLLLTGYQSQGTIGRMIQDGTKSVRINGEDVPVRAKVVFID
                     GYSGHKDSDHLIQFVESVADSVKKVFVVMGEPKSSLFLTQRLRDYLGVYAFAPEQNDV
                     VELEC"
     gene            complement(3493..3897)
                     /locus_tag="VLB02_02770"
     CDS             complement(3493..3897)
                     /locus_tag="VLB02_02770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56981.1"
                     /translation="MRKTLVLRALLLVLFIFAVNALAMKFYWYTAMLWFDMPMHFLGG
                     VFLALATGVCLSRQLLEKPFRQVLPILLISVLCFGVLWELFEYSVQTVIRFAQLANIP
                     DSFSDLFFDMLGGSIGAIFVYRLKRRYNRARE"
     gene            complement(4163..4639)
                     /gene="ndk"
                     /locus_tag="VLB02_02775"
     CDS             complement(4163..4639)
                     /gene="ndk"
                     /locus_tag="VLB02_02775"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008776717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HSE56982.1"
                     /translation="MNHPKTERSLIIIKPDALQRNLVGNIIHRFERKGLKIIGMKMMT
                     LEDAVLAEHYAHVADKPFYPRINRFMKSAPVIVIAIVGINAIGAIRTIVGPTKGYEAD
                     AGSIRGDFSMSMQSNVVHASDSVENGEIEIKRFFKEDELFSYEKIDTTYIFSDDLL"
     gene            complement(4655..6358)
                     /gene="argS"
                     /locus_tag="VLB02_02780"
     CDS             complement(4655..6358)
                     /gene="argS"
                     /locus_tag="VLB02_02780"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HSE56983.1"
                     /translation="MKENLEQALRGIAKELGIPEVAFSVEHPKELSHGDYATNLPLVA
                     AKQLGKSPKEIAELVKKKLLALGLSEVESVTVAGAGFVNITLTSAFFTSALAEILHTK
                     EFGKNNSLAGKKVLIEYTQPNPFKPFHIGHLMSNSIGESLTRIFEWSGAKVVRANYQG
                     DVGLHVAKAIYGLMQKPMPEKLAAGSIAEQAAYIGTCYAQGSSLYDESAEAKQAIDAI
                     NKQVYDRSDESVNKLYEWGRKVTLLAFEGLYQQLGTTFDHYFFESEMATVGLPLVQEN
                     KGNVFEESDGAIVFHAETYNPKLHTRVFITSAGLPTYETKELGLTKTKFETINPDLSI
                     VLTAVEQVGVIQVVREAVRLINPAWAEKMLHVTHGMMRFASGKMSSRKGNVITGESLL
                     RDAEKAVLEKMQDRAMAAAERQQVAELVGTAAIKYSILKQATGSDIVFDFEKSISFDG
                     DSGPYLQYSVTRAKSVLAKAAALHMQSKVSGSEQVTVLEKLLCRFPAVVMRVQAEREP
                     HYLTTFLTELASAFNAFYGAHQIVGEGTEYQHRLAVTEAFVQVMERGLWLLGISVPEK
                     M"
     gene            6479..7411
                     /locus_tag="VLB02_02785"
     CDS             6479..7411
                     /locus_tag="VLB02_02785"
                     /inference="COORDINATES: protein motif:HMM:NF012524.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HSE56984.1"
                     /translation="MERTTDSQYEDLLREILENGKTKAPIHTLLPENKNSGSIYALEL
                     SGKSLTYSVANGAPITPIRNLDDPKLRLWLGAIGEIVGFINGARTLKELMSYGCPEVF
                     WKNWVTKEKCAKFGLEEGDLGPGSYGPILTAFPTADGKTFNQVKAVVTQMQKMPYART
                     QTITTWYPPYALGDDEQGFPRKVVVAPCHGNFIIFNIFDGGEMHMVHDQRSADGPVGL
                     VLNLTEWFAFGMMVAYMTGLNFTQYTHQLPNPQIYDIQIEKVKELLEREPRRLPSLYL
                     HPKREIKSIFDFRTEDFELEDYDPHPKMKIPTLI"
     gene            7494..7949
                     /locus_tag="VLB02_02790"
     CDS             7494..7949
                     /locus_tag="VLB02_02790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56985.1"
                     /translation="MPELKNNIQYVDINNARGEKMIQKWQGIIADGVDPFDPQYVENY
                     LEGTVILESDHWYAFQNDHPYDNIQLQLVIVTKKFFKDSYDLPDDVWLDLKAIKNKLR
                     DQFEITGGGFVMRFGDTTLSGGTVVHLHAQIIVPAKEKKVAAWFGSGEK"
     gene            complement(7989..8834)
                     /locus_tag="VLB02_02795"
     CDS             complement(7989..8834)
                     /locus_tag="VLB02_02795"
                     /inference="COORDINATES: protein motif:HMM:NF025793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deaminase"
                     /protein_id="HSE56986.1"
                     /translation="MKEAIVCYVPVIHRSYQEFFNRYPKVDIFLVTQNLIISLDSDKA
                     EKMKRNIHALEVADIQQMLALHYGPHRRVKIINSISDLDTFQKLIMPDEDISDLILKF
                     ITKEKFEILIENRFLRYDWKAATAIKTVDPDTEVTSDEMHQSFMLLAKQVSQKSSDWW
                     RQIGVAVVCSDGTVLTAFNEHMPHADSVNIFGDPRSNLNAGELPNVCTAIHGEASIIA
                     DAARQKGVSTDGASMYVTTFPCLVCARFIRKAGIKVLYYSEGYSRLDAKEILQAHGVK
                     LVKVV"
     gene            complement(8831..9526)
                     /locus_tag="VLB02_02800"
     CDS             complement(8831..9526)
                     /locus_tag="VLB02_02800"
                     /inference="COORDINATES: protein motif:HMM:NF013621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HSE56987.1"
                     /translation="MENENVRLTYTKTTLDPNEVSILKIVGTDPSPEHRIILDRKKLI
                     WIIGVLKKEGLRIVYTGGVFDQMHDGHVKYLAEAKLRGDILIVGVDDDDLTRQRKPDQ
                     PHRPIDPLGVRLTNLVHIRSVNILTVRTTAEPLEQLIFDLLPDVAVFSKGTKDAQEQE
                     IRSQRGPYCGEIVFLEPQSGNSTTAKISKVMINGAHDLAVHLKKELDGKVDLSLLEKS
                     VGSYFTKIQGGIK"
     gene            9648..10199
                     /locus_tag="VLB02_02805"
     CDS             9648..10199
                     /locus_tag="VLB02_02805"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF012413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HSE56988.1"
                     /translation="MKIIIVVAYAILNRVIGLNGSIPWKILQDMLHFKKLTITIPESV
                     VIMGRKTFESFPKKFRPLPDRWNFIVSRNPDYTPSPTNEKTKVFTSLAAAIECAKEME
                     FKLIFIIGGGEIYKEALDQRDFTIDEVIATEVDGNFEGDTFFPDLYPGSWSEPEVLNT
                     IIKDGDKNSHDCRIVSYKQLIAL"
     gene            complement(10255..10680)
                     /gene="rplK"
                     /locus_tag="VLB02_02810"
     CDS             complement(10255..10680)
                     /gene="rplK"
                     /locus_tag="VLB02_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010018673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HSE56989.1"
                     /translation="MAKTITKKIKVRAPAGSASAAPPLGPALGQAGVNIGDFVQKFNA
                     ATADQPKGELLGVKITVYDDRSYDFDVKGPQASDLLQKAAGIQKGSGKPNTSKAGKVT
                     KDQVRQIAERKMKDLNAKDIEGAMKIIEGSARSAGIEVK"
     gene            complement(10731..11279)
                     /gene="nusG"
                     /locus_tag="VLB02_02815"
     CDS             complement(10731..11279)
                     /gene="nusG"
                     /locus_tag="VLB02_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HSE56990.1"
                     /translation="MAKQEITGERHWYAIHTYSGYENAVQRNLKQRIDSLGVQDKIFN
                     VVVPTEKKIKIKGGKRVEVEEKVYPGYVLVDMIVTDDSWFVVRNTPRVTGFVGSGVNP
                     VPLTQAEIDYLLGQMKDDTVAKHTIDLEIGEAIIITDGPFKELEGRVNEVDHERGKVK
                     VFVSLFGRETPVELDFLQIKKI"
     gene            complement(11285..11464)
                     /gene="secE"
                     /locus_tag="VLB02_02820"
     CDS             complement(11285..11464)
                     /gene="secE"
                     /locus_tag="VLB02_02820"
                     /inference="COORDINATES: protein motif:HMM:NF012793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HSE56991.1"
                     /translation="MSIAQFFHNTKAEMKHVHWPSRQHTIVFTAVVIVVSLVVGYLLG
                     AFDMLFQAILKTILF"
     gene            11579..11851
                     /locus_tag="VLB02_02825"
     CDS             11579..11851
                     /locus_tag="VLB02_02825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56992.1"
                     /translation="MKKKVLKPKKEMTIDDLAIMVAQGFEQTATKDDIRRLEQQDTEI
                     VKRLDDIDYRLGKIEANHERRIDLLEDKIQIIYTAFEKNLKIKLAK"
     gene            complement(11887..12687)
                     /locus_tag="VLB02_02830"
     CDS             complement(11887..12687)
                     /locus_tag="VLB02_02830"
                     /inference="COORDINATES: protein motif:HMM:NF016300.4"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HSE56993.1"
                     /translation="MTPFIEKYQTTIIRIIVAVGVLLTLFLLAATLGKLKEHRFIGSG
                     VSPAQTITVAGTGKVNRAPDTAKISFTVRAEDKQVKNAQQVVSGKIDAVTKVLKDIGI
                     EERYIKTDYYNSYPKYEYGTVACRAAYCPPAGTPKLVGYEVSHAITVNVKDLDKVEEV
                     LGALGTAGVSDISGPNFGFEDDKAVQREARDMAIKDAREQAQLLARSLGVKIVRLVSF
                     SENGGAYPLYRESLQGKAYDAATAAPVPALPVGDQEISANITLTYEIR"
     gene            13249..>13813
                     /locus_tag="VLB02_02835"
     CDS             13249..>13813
                     /locus_tag="VLB02_02835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56994.1"
                     /translation="MLVEQLKTRTGNQLLKVKIGGDVLVGYFTKEIKYLRPSDEKEWW
                     HAFETWGVDIESVEVKHDPFPYLREIKGVLYAFYLKDHPKAGIVGRYLIEIKNDRETY
                     EPINWECALDEKGGVTPFRWMRGAGENLQKSFEEIRSYGKEGEYSVDSFGIDQLGTIC
                     AWHAKCEKPNCECSSGWWEGNEKKVVLY"
BASE COUNT         3835 a         3247 c         2802 g         3929 t
ORIGIN      
        1 cccatcacga atatgtacag gttcccaatt gatagatttg ttttcttcaa tcaaggtatg
       61 tttgacacgc tcgtccgaca tccggatata ccacgaatcg cgcgcgtagt agatgagcgg
      121 cgttttgcag cgccagcagt gcgggtacga gtgctcgtac ttttcttttt taaacaaaag
      181 cccgcggtgt gccaaatctt tgataatctc aactgcggta ctctcatctt ttacaaactg
      241 tccctcaaga aaactggtac cttttataaa gtgcccacta tcatcaacag tgtgtagttt
      301 tggcagccca acctgcgtac cgagttcaaa gtcgtcttgc ccgtacatcg gcgcaatatg
      361 cacaattcct gtaccatcat tcgtggttac aaaatctgct gcatatactt tgtaggcatt
      421 ggtaagtttc tctttttcag ttccatttat ttgttctgag agatatggat agagtggttc
      481 gtactcaaga ccaacaagtt cactacctgt cagttcgcca agtaccgtgt actctccctc
      541 aacaccatca agtcgttctt ttgcaagaat taataattca ttttctttct cgataatgct
      601 atacgtaatc gcagctccaa cagcaagtgc cgcattccct ggtaacgtcc atggtgtagt
      661 agtccatgcc aataagtaga ccggggcagt acccccaccg aagatttttt ccatgtctgg
      721 ttgagcgaag cgacccgaag actgggaaac atctttggtg ggaattttga tcttaaattt
      781 tacatatacc gaaagatctt ttacatcagc gtagccttgc gccaattcat gcgaagagag
      841 cgccgtaccg cagcgcgggc accacggcag cactttatag tctttataca aaagtccatc
      901 tttctctact ttgcccaaaa tattccacac cgactcaatg tagttattat ggtaggttac
      961 atacggatct ttttgatcaa cccagtaacc aatacggtca gtaaagcgtg tccacagatc
     1021 aacatacatc cacacactct ctttacattt tttattaaac tcggcaatcc catactgctc
     1081 gatttctttt ttcgatttca atccgagctc tttttccact tggagctcaa ccggcaaccc
     1141 gtgggtatcc cagccaccct ttcggcgcac tgaaaatccc tgcatcgttt tgtaacgcgg
     1201 aataacatct ttaaacgcac gagcttctag atggtgaatc cccggcttgc cgtttgcagt
     1261 aggcggacct tcgtaaaaca caaatggttt gccatcctta gttttctcga gcgacttttt
     1321 gaaaatttct ttctctttcc aaaactccaa gacgcgctct tcgcgctctg ccgctgctga
     1381 tttctttatt tcgtttgtat tgttgttcat ataaaaatta aaacccgtcc gcacatagca
     1441 aaattacttg ctactatagc aaacacgctg ccagaaacaa gccaagaact tagagtgatt
     1501 ggtacttctt ttggtcttct tcaaccattg caatcacccg ctgtaccaag cgctttgggt
     1561 cggcatcaaa gataacccga tcagtcgggc gattcccctt ttcgatcata tcgcgaattt
     1621 ggtctgccat ggtccacgga ccttcaagca cccccaccgg cttgccgtct tcaaacgcaa
     1681 ttgtaaactc gtgaatagta ccaatgcgcc cgcagccaat aaatatggcg tcagagctgc
     1741 gggtaaacaa aatatcgcgc cccggaaacc caaatccggt gtagatgatt aaatccatat
     1801 agtcgagcgg caggcggtag gtttcaacat gttctttagc agttgccgct ggagagatac
     1861 caatacaaat accatgtgcc tctttggcgc ccatcgctgc ccagagcgga aagccagtgg
     1921 tagcaccggt gagcaccagc ccgccctggc gcaccagctc gcgaccaagt tcttttgctt
     1981 tttcaagcgc atcgagccca cagtgccctg tttctgcagc accggaaaca ccaaacttaa
     2041 gctttacatg cccgtgtttc tcgctatttt caagcggtga attgtagcag acgtacttat
     2101 tactgttgtt gttttttact tcttccatgt ggctagcact ctaattctac tacatcgttc
     2161 tgctcaggag cgaacgcgta cacacccagg taatcgcgca ggcgctgggt taaaaagagc
     2221 gatgatttgg gctcgcccat caccacaaac acctttttca ctgaatcggc aacgctttca
     2281 acaaactgga tcaggtgatc cgaatctttg tgccccgagt agccatcaat aaacaccacc
     2341 ttcgcccgaa ccggtacatc ttcgccgtta atccgtaccg acttcgttcc atcttgaatc
     2401 atccgtccga tagtgccttg cgattggtag ccggtgagca gtagcgtgtt actttcatca
     2461 ggaagatagt ttatttcatg gtggagcact cgtccgccgt tcgacatccc cgagccagca
     2521 atgacaatct tcgggttcgg cacatgcaaa attgattttg attcttcagt aagtaaggtg
     2581 agtttcaaac cagggaaatt aaaaatgtca tcgccttcgc caatagctgc gcgagcttca
     2641 gcattaaagt agcgtgtgtg atctttatac acgcgggtaa gttttatcgc caatggtgaa
     2701 tctaaaaaca ccggcatccg cggaatgcga tcgtgctcaa ccaggctgtc aatttcgtac
     2761 aaaagctcct gcgctcgctc gagcgagaat gttggaatca ccagtgcacc atttttttta
     2821 aagttatcct cgatcacccc ctccaaaagt ttccggcgtt cgttgcgaga aatgtgattc
     2881 cgatcgccgt agactgattc cataatgagg taatcaacat tttggagcac ctcagtgtcg
     2941 tttaagagtg gtgacggcga gttgccgagg tcgccagtaa agacaatttt tttattgtta
     3001 taaataatct cgagcattcc cgaccctaaa atgtgccccg catcgcggaa ccgaaagaga
     3061 aatggatcga tccgaatttc ttggtgataa tcaaccactt gccaatgagc aaatactttt
     3121 tttaaaatat ccgggctgta tatttttccg agcagttcgt tatcgctgcg cgagagaata
     3181 tttgctgtat cttcaagcat tagctcggca atatctttgg taggaatggt tgagtagata
     3241 gtgcccttga agccatcgtg gataagtttt ggaatccgac caacgtgatc gatatgggca
     3301 tgggtgataa aaagaaaatc aatacttgtc ggatcatagg taaacgcttc ccagtttgat
     3361 tcatcggcaa gttttgtacc ctgcgcgagc ccgcagtcaa ctaagaaccg ctttccctcg
     3421 ccttcgagta aaaaattggc gccggttact gaaccagtgc cgccggcaaa tgttaatttc
     3481 agtttttttg attcactcac gtgctctatt gtaccgcctc ttgagacgat agacaaaaat
     3541 tgcgccgata cttccgccga gcatatcgaa aaacaaatca gaaaaagaat caggtatgtt
     3601 tgcgagctgt gcaaatcgga taaccgtctg cactgaatat tcaaataatt cccacaaaac
     3661 accgaagcag agcactgaaa taagtagtat aggcagcacc tgtctaaatg gcttctcgag
     3721 cagctgtctg ctcaagcata ctcctgttgc aagtgccaaa aatacacccc ctaaaaaatg
     3781 cattggcata tcgaaccaca acattgctgt ataccaatag aacttcatcg cgagcgcatt
     3841 caccgcaaag ataaaaagta ctaacagcag tgcgcggagt actaatgttt ttctcatcca
     3901 cccattatcg cacattccgc aaacgaaaaa cccatccttt acaggacgag ttcctcgtta
     3961 cggctactac ctaattatta acttagcctt tttgctaagt gggcacctgg gaagaggcga
     4021 cataaaaaga tttcaccaat tccactgcag ttccctgatt cagcgcgttt ctagttaata
     4081 aatagggtaa ccgtatactc agtatagatg cctgattctg ttgcacaact agacacctag
     4141 ggaaaaatgt ctccgaacgc actcagagta aatcgtcaga gaaaatatac gttgtatcga
     4201 tcttctcata gctaaataat tcatcttctt tgaagaatcg ttttatttct atttcgccat
     4261 tttcaacaga atcagaggcg tgcaccacgt ttgactgcat actcatcgaa aaatctcccc
     4321 tgatgctccc tgcatctgct tcataccctt ttgttggacc aacaatcgtg cgaattgcgc
     4381 caatagcatt gatacccaca atcgctataa caattactgg tgccgatttc ataaatcgat
     4441 tgatgcgagg atagaacggc ttatcagcaa catgtgcata gtgctctgct aacacagcat
     4501 cttcaagcgt catcatcttc ataccaataa ttttcaaccc tttccgttca aaacggtgga
     4561 taatattccc taccaagttc cgctggagcg catcgggttt aataataata agtgatcgtt
     4621 ctgtttttgg gtgattcata cgggagctat tctactacat tttttcaggg acgctaattc
     4681 ccaacagcca caagccgcgt tccatcacct gcacaaacgc ttcagttact gctaggcgat
     4741 gctggtattc ggttccctcg cccacaattt ggtgcgcacc ataaaaggca ttgaacgcac
     4801 tcgcgagctc agtcaagaag gtggtgaggt agtgcggctc acgctcggct tgcactcgca
     4861 taactactgc aggaaatcga cacagcagtt tttcaagcac ggttacctgt tcactgccac
     4921 tcactttgct ctgcatatgg agcgctgccg cttttgcaag cacactttta gcgcgagtaa
     4981 cactgtactg caggtacggt cccgaatcgc catcgaacga aatagatttc tcaaagtcaa
     5041 acacaatatc gctcccggtt gcctgcttta aaatcgagta cttgatcgct gcggtaccaa
     5101 ccagctcggc aacctgctgc cgctcagctg ctgccattgc tcgatcctgc attttctcaa
     5161 gcaccgcttt ttctgcatcg cgcaagagtg attctccggt aatcacattc cccttacgcg
     5221 aggacatctt gccgctcgca aagcgcatca tgccgtgcgt tacgtggagc attttctctg
     5281 cccacgccgg attaatgagc cgaactgctt cgcgcactac ctgtatcact cctacctgtt
     5341 caacagcagt aagcactatg gagagatcag ggttgattgt ttcaaatttt gttttggtga
     5401 gcccgagctc tttcgtctcg tacgtcggca atcctgcaga ggtaataaat acccgcgtgt
     5461 gcagttttgg attatatgtt tctgcatgga acacaatcgc cccgtcagat tcttcaaaca
     5521 cattcccctt attttcttgc accagtggca acccgactgt tgccatttcg ctctcaaaaa
     5581 agtagtggtc aaacgtggtg cccagctgct ggtataaccc ctcaaatgcg agcagggtta
     5641 cttttctccc ccactcgtag agtttattca ccgattcatc gcttcggtcg tacacctgct
     5701 tgtttattgc atcaatcgcc tgcttcgctt ccgcgctctc gtcatacaag ctcgaaccct
     5761 gcgcgtaaca cgtaccaatg taggctgcct gctcggcaat actccccgct gccagcttct
     5821 caggcattgg tttttgcatc aatccataaa tggcttttgc aacgtgcaag ccaacatcgc
     5881 cctggtagtt tgcccgcacc acttttgccc cgctccactc aaaaattctg gtgagcgatt
     5941 caccaatcga gttactcatc aggtgcccaa tatggaacgg cttgaagggg tttggctgcg
     6001 tgtactcgat cagtactttt tttccagcca gagaattatt ttttccgaac tctttcgtat
     6061 gtaaaatttc ggcaagtgca ctagtaaaaa aagcactagt aagggtgata ttcacaaacc
     6121 cagcaccggc aacagttacc gattcaacct ctgacaaccc cagtgcaagc aatttctttt
     6181 tcacgagctc ggcaatttct tttggactct tccccaactg ctttgctgcc accagcggca
     6241 ggttagtcgc gtagtcgcca tgcgagagct cctttggatg ctctaccgaa aaagccacct
     6301 cggggatacc taactccttc gcaatccccc tgagtgcctg ttcaagattt tctttcatac
     6361 ggagagtcta acatcaaagg tttaaataac aaccgctgtt gacttttttg ctcattgcta
     6421 tagagtttgc aaaaaacaaa ttatggaaat aaaacaacct gttctttaca aaccgtatat
     6481 ggaacgcact acagactcac agtatgaaga tctcctcaga gagattttag aaaatggaaa
     6541 aacaaaagcc cctattcata cactgcttcc ggagaataaa aattcaggca gcatatatgc
     6601 ccttgaactt tctggaaaat cactcaccta ctctgtagcc aatggtgcac ctattacccc
     6661 tattcgaaac cttgatgatc ctaagttaag actatggttg ggagcaattg gagaaattgt
     6721 cgggtttata aatggagcta ggacactaaa agagctcatg tcgtatgggt gtccagaggt
     6781 tttctggaaa aactgggtaa caaaagaaaa gtgcgcaaaa tttggactgg aagaaggaga
     6841 tcttggacca ggatcgtacg gtcccatact gactgctttt ccaacagcag acggaaaaac
     6901 attcaaccag gtgaaagcgg tggtaaccca gatgcaaaaa atgccgtatg cacggacaca
     6961 gacaattacc acctggtatc ctccgtacgc gcttggagac gacgaacaag gatttccaag
     7021 aaaagttgtt gtagcaccct gccatggaaa ttttataata tttaatattt ttgatggcgg
     7081 agagatgcac atggtgcacg atcaacgatc tgctgatggt cctgtcggac tggtacttaa
     7141 tttaactgaa tggtttgcgt ttggcatgat ggtcgcctat atgactggtc taaactttac
     7201 ccagtacaca catcagctcc ctaatccgca aatttatgac attcagattg aaaaagttaa
     7261 ggagctcctc gaacgtgagc cacgccggtt gccaagttta taccttcacc caaaacggga
     7321 aataaaatca atttttgatt ttagaacaga agattttgaa cttgaggatt atgatccgca
     7381 tccgaagatg aaaatcccaa cattaattta gcaatgttta cgttttaaat ttgaagtcta
     7441 cctacgggtg gacttttttt ctttttccgg tagagtatgg aaaattcatt catatgcctg
     7501 aactaaaaaa taatattcag tatgttgata tcaacaatgc ccgtggagaa aaaatgattc
     7561 aaaaatggca agggattatc gctgatggtg tagacccttt tgatccccag tatgtagaaa
     7621 attatcttga aggaacagtt atcttggaat cagatcactg gtatgcattt caaaacgatc
     7681 atccatatga caatattcaa cttcaactag taattgttac gaaaaaattc tttaaggaca
     7741 gctacgacct accagatgat gtttggcttg accttaaagc aataaaaaat aaactgagag
     7801 atcaatttga aattactggt ggtggattcg tcatgcgctt tggagataca acactttccg
     7861 gtggtactgt cgtccacctt catgctcaaa ttatcgtccc cgcaaaggaa aagaaggttg
     7921 ctgcatggtt cggatcagga gagaaataaa taaagcccaa atacaattat gtatttgggc
     7981 ttattaattt acactacttt tactagtttc actccgtgag cctgtaagat ttcctttgca
     8041 tcaagtcgag aatatccttc gctatagtaa agaactttaa tgccagcctt cctgataaat
     8101 cgggcacaaa ctagacatgg aaatgtagtg acatacattg atgcaccatc agttgataca
     8161 cccttttgcc gggcagcatc tgcaataata cttgcttcac catggattgc agtacacaca
     8221 ttaggtagct ctcctgcatt taaatttgaa cgtggatcac caaatatatt aaccgaatca
     8281 gcatggggca tatgctcatt aaaggcagtg agtacagtac catcactgca gactacagca
     8341 acaccaattt gtcgccacca gtctgaactt ttttgagaaa cctgttttgc aagtaacata
     8401 aaactctgat gcatttcatc tgaagttacc tcagtgtccg gatctactgt cttaattgca
     8461 gttgctgctt tccagtcata gcggagaaac ctattctcaa tcaggatctc aaatttctct
     8521 tttgtgataa atttgagaat cagatcactt atatcttcat cgggcataat gagtttctga
     8581 aacgtatcca gatctgaaat agagttgatt attttaactc tgcgatgtgg tccatagtgt
     8641 aaggcgagca tctgctggat atctgctact tctaatgcgt gaatatttcg cttcatcttt
     8701 tctgccttgt cactatctag ggatataatc aagttttgtg taactaggaa aatatctacc
     8761 tttggatatc ggttaaaaaa ttcctgatag ctgcgatgga taacaggcac gtagcaaaca
     8821 atagcttctt tcatttaata cctccttgga ttttagtaaa ataactacct actgattttt
     8881 ccaacagcga cagatcaact ttgccatcta gttctttttt tagatgcacc gcaagatcat
     8941 gtgctccatt aatcatcacc ttgctaattt ttgctgtagt tgaatttccc gattgcggtt
     9001 ctaaaaacac tatctctcca caatatggac cacgctggct tctaatttct tgttcctgtg
     9061 cgtctttagt cccttttgaa aatacagcca catcaggaag aagatcaaaa ataagctgtt
     9121 caagcggttc cgcggtggtt ctcacggtga gaatattaac tgatcgaata tgaaccaaat
     9181 tagtaagcct gacaccaagc ggatcaatag gacgatgtgg ctgatctggt tttcgctgtc
     9241 tggtaaggtc atcatcgtct actcctacta tgagaatatc tccgcgcaat ttggcttctg
     9301 ctaaatattt cacatgacca tcatgcattt gatcaaatac gccaccagtg tagacaatgc
     9361 gaagtccttc ttttttaagt actccaataa tccatattaa tttcttgcga tcaagaatga
     9421 ttctgtgctc aggacttgga tcagtaccaa caatttttaa tatactaact tcatttggat
     9481 caagtgtagt tttagtgtaa gtgagcctaa cgttttcgtt ttccattgta aaagaatttt
     9541 tgttataaat tttgttttcc ccaatactat gctccctagc cacaaaggca agattgacga
     9601 ttacaagttt acatggcagt atcaaagcaa attaaaaaca tacgagtatg aaaataatca
     9661 ttgttgttgc atacgccata ctcaatcgag taattgggtt aaacggctcc ataccatgga
     9721 aaattctgca agatatgctt cacttcaaaa aacttactat taccatccct gaaagtgtgg
     9781 tgattatggg cagaaaaact tttgaatcgt ttccgaagaa atttcgccca cttcccgacc
     9841 ggtggaactt tattgttagc cggaatccag actacacccc cagtccgaca aatgaaaaaa
     9901 caaaagtatt cacttcttta gctgccgcaa tcgagtgcgc caaagaaatg gaatttaagc
     9961 tgatatttat tatcggtggt ggagaaattt ataaagaagc acttgatcaa cgtgatttta
    10021 caatcgatga agtaattgca acagaagttg atggtaattt tgagggcgac acattcttcc
    10081 ctgacctcta ccctggaagc tggagtgaac cagaagtact aaacactatt atcaaagatg
    10141 gggacaagaa ttcccatgat tgtagaatag tgagttacaa acaattgatt gctctttaaa
    10201 aaaaaccaaa gagcaaaata ccccgtttta gacggggtat tttttatttc aaacttattt
    10261 cacttcaatc cctgcgcttc gggctgagcc ttcgatgatc ttcatcgcgc cctcaatgtc
    10321 tttggcattt aagtccttca ttttgcgttc tgcaatttgg cgaacttggt ctttggtcac
    10381 cttccctgcc tttgaggtgt ttggcttccc tgaccctttc tggatacctg ccgccttttg
    10441 gagcagatca gatgcctggg gacccttgac gtcgaaatcg tatgagcggt catcgtagac
    10501 cgtgattttt actccgagca gttcgccttt tggctggtct gcggttgcag cgttaaactt
    10561 ctggacgaag tcgccgatgt ttacccctgc ctgcccaagt gccgggccaa gcggtggtgc
    10621 agcgcttgct gaccctgccg gtgcccgaac cttaatcttt ttcgtaattg ttttagccat
    10681 atgattggtt cataatatat caatttctat ttctgtccag cactcgtttg ttaaatcttt
    10741 ttgatctgca agaagtcgag ctcgactggc gtttcgcgac cgaaaagcga cacaaacacc
    10801 tttaccttgc cgcgttcgtg gtcaacctcg tttacccgcc cctcaagctc tttgaatggt
    10861 ccatcggtaa taataatagc ctctccaatt tcaaggtcga tggtgtgttt tgcaactgta
    10921 tcgtccttca tttgtccgag gaggtagtcg atttcagcct gggtgagcgg tactgggttt
    10981 acccctgaac caacgaaacc cgttacccgc ggcgtgttgc gcaccacaaa ccacgagtcg
    11041 tcagtcacga tcatatccac gagcacgtag ccggggtaca ccttctcttc aacctcaaca
    11101 cgcttgccgc ctttgatttt gatcttcttt tcagttggca ccacaacgtt aaaaatttta
    11161 tcctgcacgc ccagagaatc gatgcgctgt tttaagttgc gctgcactgc gttttcgtac
    11221 cccgagtaag tatggattgc gtaccagtgg cgttcgccgg tgatttcttg ttttgccata
    11281 tatgttagaa gagaattgtt ttaaggattg cctgaaagag catatcgaac gcgcccaaca
    11341 ggtagccaac gaccaaagaa acaacaataa cgaccgcagt aaacacaatc gtgtgctggc
    11401 ggctcggcca gtgcacatgc ttcatttctg cttttgtatt gtggaaaaat tgcgcaatgc
    11461 tcatagcgtt ttgctcttac taaaaaccgc cccggagggc taatagtgtg ataatagccc
    11521 ggctttgaga ataagtcaat tgactccccc taccgctcat ttatactata ctcctcacat
    11581 gaagaagaag gtattaaaac cgaaaaaaga gatgaccatc gatgacctgg caattatggt
    11641 tgctcaaggt tttgagcaaa ctgccacaaa agacgatatc cgtcgccttg agcagcaaga
    11701 tactgaaata gttaagcggc ttgatgatat tgattaccgt ctcggaaaaa ttgaagcaaa
    11761 ccatgaacgg agaattgatt tacttgaaga taaaatacaa attatttaca ccgcctttga
    11821 gaaaaaccta aaaatcaaac tggcaaaata aaaatccctc ggttgaggga tttttattta
    11881 ttccaactag cggatttcgt acgtcagcgt tatgtttgcg ctgatttcct gatcgcccac
    11941 tggcagtgcg ggcactggcg cagcagtagc tgcatcatac gccttacctt gaagtgattc
    12001 acggtagagc ggatacgctc ccccattttc agagaacgat accagccgca caatttttac
    12061 cccgagtgat cgcgccaaga gctgcgcctg ctcgcgggca tccttgatag ccatatctcg
    12121 tgcttcgcgc tgcactgctt tatcatcttc aaaaccgaaa tttggaccac tgatatcgct
    12181 cacgccggca gtaccaagtg cgccgagtac ttcctcaact ttatcaaggt cttttacatt
    12241 caccgtaata gcatgtgaga cttcataccc tactagtttt ggtgttcccg caggcgggca
    12301 gtacgctgct cggcacgcaa ccgtaccata ctcgtacttt ggatacgagt tatagtaatc
    12361 ggttttaatg taccgctcct cgataccaat atctttcagc actttggtga ctgcatcaat
    12421 ttttccgctc actacctgct gtgcattttt tacctgtttg tcttcagcac gaacggtaaa
    12481 cgaaattttt gctgtatcgg gcgcgcggtt tacttttccg gtacctgcta cggtaatagt
    12541 ttgcgccggc gaaactcctg aaccaataaa acgatgttct tttaattttc cgagcgttgc
    12601 tgccagcaaa aacaaggtca gcagcacgcc cactgccaca attattctaa taatagttgt
    12661 ttgatatttt tctataaatg gggtcataag tggagtatac cagaaccgta tactattaca
    12721 gggtggtaat ttttacccct ataaaaatcg cttttagggt ggtattgaca aaatgcttta
    12781 aatagtatac gattagatcg agaatccatt cttaaacctt taaaacctac aactatgcga
    12841 gttaatcttg tatgcaccga atttcatctt tctcgtatgc ccaaaaacag aatgaagaaa
    12901 cttatccata ctctttcagt aactgcagtc cctgccgaaa aatcaggaca tcttcttcat
    12961 ccaattacta attggcaact acatggggag ctaccgaagg gagtgaagat taaaaatctt
    13021 cactcgggca gttataaaat attttacaaa agaccataat atatagtatt taactaaaaa
    13081 atcccgcagc aattagttgc ggttttttat gtatttcgta aaccgaaata cttgacaaaa
    13141 gtcgatattc ggtatataat tgaaagagat aatttagtgc acttacctgc acaaccggac
    13201 aagccgaaaa ccgggtaaac aataagtaag caaatcaaat tctttaacat gttagtagag
    13261 caactgaaaa caagaacagg aaaccaatta ttaaaagtaa aaattggagg tgatgtcctg
    13321 gttggttatt tcacaaaaga gataaagtac cttcgcccat ccgatgaaaa agaatggtgg
    13381 catgcttttg aaacctgggg agttgatatt gaatcagttg aagtaaaaca tgatccgttc
    13441 ccatatttaa gggaaataaa aggcgttctt tacgcattct atctgaaaga tcacccaaaa
    13501 gctggtatcg tcggtcgtta tttaatagaa ataaaaaacg atcgggaaac atatgaacca
    13561 attaactggg agtgtgcatt ggatgaaaaa ggtggcgtaa cacctttcag gtggatgagg
    13621 ggtgcaggag aaaacctgca gaaatcattt gaagaaattc gctcctatgg gaaggagggt
    13681 gaatattctg tagacagttt tgggatagat caacttggca ctatttgcgc ctggcacgct
    13741 aagtgcgaaa aaccaaactg cgagtgcagt agtggttggt gggaaggtaa tgaaaaaaag
    13801 gttgttctat att
//
LOCUS       DATIBQ010000083         4944 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U16294
            k141_1956143, whole genome shotgun sequence.
ACCESSION   DATIBQ010000083 DATIBQ010000000
VERSION     DATIBQ010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935789
            Sequence Read Archive: SRR25158331
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 4944)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4944)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:58:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 637
            CDSs (total)                      :: 603
            Genes (coding)                    :: 601
            CDSs (with protein)               :: 601
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4944
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1956143"
                     /isolate="SMAG_U16294"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(169..251)
                     /locus_tag="VLB02_02840"
     tRNA            complement(169..251)
                     /locus_tag="VLB02_02840"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(215..217),aa:Tyr,seq:gta)
     gene            complement(294..2978)
                     /locus_tag="VLB02_02845"
     CDS             complement(294..2978)
                     /locus_tag="VLB02_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020374678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HSE56995.1"
                     /translation="MPPLTNFTTKAKEAIKRAHELAIERGQNNVNPLHLLAALLTQEE
                     SSVIAILERLDVDTILLTDSVLEAIEAPESKNTMSPSYQMFVTGDLAQVFEHSLRVAQ
                     ELKDNFVSTEHLLVALIDISSQSQEVIGKFKITRGDIIRVVEELRQNRGADAQGSKKM
                     RMITKFTRNLTKLAREDKLDPVIGRDAEIMRIMQILSRRTKNNPILIGEPGTGKTAVV
                     EGLANRIAHGDVPESLKEKEVVSLDLGSLIAGTKYRGEFEERLKNILKEMERMEGKII
                     LFIDEIHTIVGAGAADGSLDAANMLKPALSRGEIRAIGATTLKEYQKHIEKDPALARR
                     FQPVYVEEPSPEDAIAILRGLKEKYELYHSVHITDDAIVSAVQFSTRYITNRYLPDKA
                     IDLLDEACSSLRMALENKPPALEEATRKIMRLQIESEALKKDIEQGTEAKTARDRLKE
                     IESDIANIQDKTKELELKWKNEKGLLDEISRVKKELDTMRLEADAAQMRGDLGRTAEI
                     RYGYVPMLQKELDQKLGKLKKLQKSRRILKEEITSEDIAEVVARWTGIPLTRMLEEER
                     EKLVRMEAELKHRVVGQNQALEKIANVVRRSRAGISDPNKPIGSFIFLGPTGVGKTEL
                     TKALAEFMFNDDKALIRIDMSEYMERHAVSKLIGSPPGYVGYDEAGQLTEAVRHRPYS
                     VVLFDEIEKAHPEVFNILLQVLDDGQLTDSKGRKVNFKNAIIIMTSNIGSQYIQKMET
                     IGFHNHSEVEEYSEMKEKVLDTLKDHFRPEFLNRIDEIVVFDILSQEAIKSIVSLRVA
                     TVVERLNTKGITVKLTDDALSYLAKEGYNPHYGARPLNRLVQVKILNPIATGIISRHI
                     AEGDSVTVSFRNNDIHVDVQKGKRRSSFDKKAIEANAS"
     gene            complement(3087..4277)
                     /locus_tag="VLB02_02850"
     CDS             complement(3087..4277)
                     /locus_tag="VLB02_02850"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HSE56996.1"
                     /translation="MFHFRKKTTYLDYASATPLDRTMWRRYQPLPATVLAANPGALHQ
                     DGVRAKRALEAARASVACDVGAHADEIIFTSGATESNTLALIGAAEQLAAAGMQYEDL
                     HIITTALEHKTVSVAAQLLLARGAHVSVLQLDERGIIDPKKLRTLFTQKTTIVSCVYV
                     SSDIGTIQPIREIAKEIREWKKTHPDQTILFHVDATQAVTSCLLNVEQLHVDLMTLGS
                     TKLYCPRGVGVLFKRRGVGLKPVHAGGSQEFGLRPGTEPLDIIHRFAYALHYARSIQK
                     QESERLTVLQTYFETELCGLLPTLRISGEGAPRVPFITHAVIPEIDSELLVLELDARG
                     ILVSSKSACMNDEPGASGIVEQLYGTATLGAVRFSFGRQTKKAELVRAIEAFGAVLKK
                     YGKI"
     gene            4335..4811
                     /locus_tag="VLB02_02855"
     CDS             4335..4811
                     /locus_tag="VLB02_02855"
                     /inference="COORDINATES: protein motif:HMM:NF025935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4446 family protein"
                     /protein_id="HSE56997.1"
                     /translation="MASATIPLLFIIAGVLAVATVWLTVSHFRLRRKLTAFMQGNNAA
                     SLEESLKHITDRNVIVEKTLSAHKEALEHLNGRLAKSVRGLSLVRYNAFPDTGGLQSF
                     ASGLLDEHGNGFILSVVTSRSHVGIYAKPVTAYKPAVELADEEARALSEAKEHQTI"
     gene            4847..>4944
                     /locus_tag="VLB02_02860"
     CDS             4847..>4944
                     /locus_tag="VLB02_02860"
                     /inference="COORDINATES: protein motif:HMM:NF012239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE56998.1"
                     /translation="MGHIDHGKSTLLDYIRKTSVVAGEAGGITQHLS"
BASE COUNT         1202 a         1204 c         1148 g         1390 t
ORIGIN      
        1 gtagtttatc ccatccccgt tctttaagat acacgtgaat tttttgttca aaatccttca
       61 tacttctcaa tcctagcact cttttttaaa ttaaaaagag ctcctttttg tttgctccct
      121 ttcacagcaa tcaaaaaagc ctagcgagaa gctaagcttt tttgattgtg cgccgggcag
      181 gatttgaacc tgcgtagccc gaaggcgcct gatttacagt caggtgtaat tgaccactct
      241 accaccgacg catggccgct ctgccaaaag gcaaaacggc tgatatgaag agcttacgaa
      301 gcattcgcct cgatagcttt tttatcgaaa gaactacgtc tctttccctt ctgaacatca
      361 acatgaatat cgttgttccg gaacgagacg gtgacggaat caccttcggc aatgtgccga
      421 gaaataattc ctgtcgcaat cgggttgagg attttcacct gtaccaaacg attgaggggg
      481 cgcgccccgt agtgcggatt atacccttct tttgcaaggt aggaaagcgc atcatcggtt
      541 aactttacgg taataccctt agtgttgagc cgctctacaa cagttgcaac acgcagcgac
      601 acaatagact taatcgcctc ctgcgagagg atatcaaaga ctacaatttc gtcaatgcgg
      661 ttcaaaaatt ctggtcggaa gtggtctttc aaggtatcaa gcaccttctc tttcatctca
      721 ctgtactctt ctacttcaga gtggttgtga aaaccgatcg tttccatttt ttgaatgtac
      781 tgactaccga tgtttgaggt cataataatg atggcatttt taaaattcac cttgcgccct
      841 tttgaatcag tcagctgtcc atcatcgagc acctggagca aaatgttaaa cacttctgga
      901 tgtgccttct ctatttcatc gaagagcacg accgagtacg ggcgatgtcg caccgcttca
      961 gttaattgcc cggcttcatc ataaccaacg tagcccggcg gtgaaccaat gagttttgaa
     1021 accgcgtgcc gctccatgta ctcgctcatg tcaatgcgga tgagtgcttt gtcgtcgtta
     1081 aacataaact cggcaagcgc tttggtgagc tcggtttttc cgactccggt tggaccaaga
     1141 aagatgaacg agccaattgg tttgtttgga tctgaaatcc ccgcacgaga gcgacgaacc
     1201 acgttagcga ttttttcgag cgcttgattt tgccccacca ctcggtgctt gagctcggct
     1261 tccatccgca ccaatttctc gcgctcttct tcaagcatgc gagtgagcgg aatacccgtc
     1321 cagcgagcaa ccacttcggc aatatcttcc gaggtaattt cttctttcag aatgcggcgt
     1381 gatttttgga gcttctttag tttgcctagt ttttggtcaa gctctttttg gagcatcggc
     1441 acgtagccgt agcgaatttc tgctgtgcga ccaaggtccc cccgcatctg cgctgcatca
     1501 gcttcaagtc gcatggtatc gagctctttt ttaacgcggg aaatttcatc gagcaacccc
     1561 ttttcgttct tccatttcaa ttcaagctcc tttgttttat cttgaatgtt tgcaatatct
     1621 gattcaattt ctttcaagcg gtcgcgggca gtttttgctt cagtcccctg ctcaatatct
     1681 tttttgagcg cctcactctc aatttgcagg cgcataattt ttcgagtcgc ctcttcaagt
     1741 gccggcggtt tattctcgag cgccatgcgc agcgacgagc aggcttcatc gaggagatcg
     1801 attgctttgt caggcaagta gcggttggta atgtagcggg ttgaaaactg cactgctgaa
     1861 acgatggcat cgtcagtaat gtgcacgctg tggtagagct catacttttc tttcaagccg
     1921 cgcaaaattg caattgcatc ttcaggagac ggctcttcaa cgtagaccgg ctgaaagcgc
     1981 cgcgcgagcg cagggtcttt ttcaatgtgc ttttggtatt ccttcagggt tgttgcacca
     2041 attgcccgaa tttcaccgcg ggagagggcg ggcttgagca tgttggcagc atcgagcgaa
     2101 ccgtcagcag caccagcgcc gacgatggtg tgaatttcat caataaagag aataattttt
     2161 ccctccatgc gctccatttc tttgagaata ttttttaggc gctcttcaaa ctcgccacgg
     2221 tatttggtgc cggcaatgag cgagcctaag tcaagcgaga caacttcttt ttccttaagt
     2281 gattcaggta catcgccatg tgcaatgcgg tttgcgagcc cttcaacaac ggcagtcttc
     2341 cccgtccctg gctcaccaat taaaatagga ttgtttttgg tgcggcgcga gaggatctgc
     2401 ataatgcgca tgatttctgc atcgcgaccg atcaccgggt cgagcttatc ttcacgggcg
     2461 agcttggtta agttgcgagt aaacttggtg atcatccgca tttttttgct gccctgtgcg
     2521 tcagcaccac gattctggcg gagctcttca acaacacgga taatatcgcc gcgagtaatt
     2581 ttaaattttc caatcacctc ttgcgattgg cttgaaatat cgatgagcgc aacaaggaga
     2641 tgctcggttg aaacaaaatt atctttcagt tcttgtgcaa cacggagcga gtgttcaaat
     2701 acctgcgcta aatcgccggt aacaaacatt tggtacgaag ggctcatggt attttttgat
     2761 tcaggcgctt caattgcctc gagcactgaa tcggtgagca agatagtgtc aacatccaaa
     2821 cgctccaaaa tagcgatcac ggatgattct tcttgggtga gaagcgccgc gagcaggtgg
     2881 agtggattca cgttgttttg cccccgttca atcgcaagct cgtgtgcccg cttaatagcc
     2941 tcttttgctt ttgtcgtaaa attagtgagt ggtggcatag tataagtagt atgcgatagt
     3001 caaattccaa taatcaatta tggtgcagaa atacagtgga cagcggtcat tcggtgtgtc
     3061 ggtatacggt aactatagca tagtggtcaa atttttccgt atttttttag cactgctccg
     3121 aatgcttcaa tcgcccttac caattcagct ttttttgtct gccgaccgaa cgaaaaccga
     3181 accgctccca atgtagcagt accatagagc tgttctacaa ttccagacgc gccaggctca
     3241 tcattcatac aggcgctttt tgatgaaaca agaataccgc gagcatcaag ttcgagtact
     3301 aagagctcag agtcaatttc aggaattact gcgtgggtaa taaagggtac tcgcggtgcg
     3361 ccttcaccgc taatgcgtag agtaggcagt agtccacaga gttctgtttc aaaatacgtt
     3421 tgcagaacag tgagccgctc tgattcttgt ttttggattg aacgcgcata gtgaagcgcg
     3481 tatgcaaatc ggtggattat atcgagcggc tcagtccctg ggcgaagtcc aaattcttga
     3541 ctcccccctg catggactgg cttgagccca actccacgac gcttaaacag cactccaacc
     3601 ccgcgcggac aatatagttt agtgctcccg agagtcatca gatcaacatg taattgttct
     3661 acattcaaca gacaactggt tactgcttgc gttgcatcaa catgaaagag tatggtttgg
     3721 tcaggatgcg tctttttcca ctctcttatt tcttttgcaa tctcgcgaat tggctgaatg
     3781 gtaccaatat cgcttgagac gtacacacaa gaaacaattg ttgttttctg agtaaaaagt
     3841 gtgcgcagtt tttttggatc aattattccc cgctcatcta gttgcagtac tgaaacatgt
     3901 gcgccgcgtg ctagaagcag ctgtgctgct actgaaactg ttttatgctc gagcgcagtg
     3961 gtaataatgt gcaagtcttc gtactgcatc cccgctgcag caagctgttc ggcagcacca
     4021 ataagtgcaa gggtatttga ttcagtcgca cccgaagtaa aaataatttc gtctgcgtgt
     4081 gctcctacat cgcaggcaac tgatgcacgt gccgcttcga gcgctcgctt tgctcgcacc
     4141 ccatcctgat gcagtgcccc tggattagca gcaagtactg tcgcgggtag cggctgatag
     4201 cggcgccaca tagttctatc gagtggtgtc gctgaagcgt aatcgagata tgtagttttt
     4261 ttacgaaaat gaaacatggc gtactaaaat aggctgcttg aaaaaaggtt gattttctta
     4321 tataatgatg cgctatggct tctgctacta taccacttct ttttataatt gctggcgtat
     4381 tggcagtggc aacagtatgg ctcacggttt cgcactttcg gctgcgacgt aaacttacgg
     4441 catttatgca gggcaataat gctgcttctc ttgaggagag cttaaaacat attactgaca
     4501 gaaacgtcat tgtcgaaaaa acactgagtg cccacaaaga agcacttgag catttgaatg
     4561 ggcggcttgc aaagagcgtt cgtggacttt ctctggtgcg atacaacgca tttcctgata
     4621 ccggcggttt gcagagtttt gcctcggggc ttcttgacga acacggcaat ggctttatcc
     4681 tttcagtagt aaccagccgc agccacgtgg gtatttacgc aaaaccagta actgcataca
     4741 aacccgctgt cgaactcgcc gatgaagaag cacgagccct cagtgaagca aaagaacacc
     4801 aaacgatatg agtaccacca ttccccgccc ccctatagtt gttattatgg gtcacatcga
     4861 ccacggcaaa tcaacgctcc tcgattacat tcgcaagacg agcgtcgttg ccggtgaagc
     4921 cggtggtatt acccagcacc tctc
//
LOCUS       DATIBQ010000084         2568 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U16294
            k141_1077127, whole genome shotgun sequence.
ACCESSION   DATIBQ010000084 DATIBQ010000000
VERSION     DATIBQ010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935789
            Sequence Read Archive: SRR25158331
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:58:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 637
            CDSs (total)                      :: 603
            Genes (coding)                    :: 601
            CDSs (with protein)               :: 601
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2568
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1077127"
                     /isolate="SMAG_U16294"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(150..1223)
                     /gene="recA"
                     /locus_tag="VLB02_02865"
     CDS             complement(150..1223)
                     /gene="recA"
                     /locus_tag="VLB02_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HSE56999.1"
                     /translation="MPKKPSVPKELKTTGKTSIEETIRSIQTKFGEGSIMKLGDTPHV
                     DVNVIPTGSIGLDSALGVGGLPRGRIIEIFGPESSGKTTLALHVVAEAQKLGGICAYI
                     DAEHAMDPIYAGKIGVNINELLISQPDTGEQGLEITESLVRSGKIDVIVIDSVAALTP
                     KDEIEGEMGQQHVGKQARLMSQALRKLTAIVAKSKTVVIFINQIRMQIGVMFGNPETT
                     PGGKALKFYTSVRIDIRRIAQIKKGEEVVGSRTRVKVVKNKVASPFRQTEFDIIYGEG
                     ISLEGEIMALGEKIGIITKPGGTSYLYTRPESSKKEEREVKLGRGYDATRTALKEDPK
                     LRDEILKEIKNRFNEIQPMSSGE"
     gene            complement(1261..1665)
                     /locus_tag="VLB02_02870"
     CDS             complement(1261..1665)
                     /locus_tag="VLB02_02870"
                     /inference="COORDINATES: protein motif:HMM:NF020700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE57000.1"
                     /translation="MKIRHLPLPHGKSIALLGLCFALIGIIFYNTRDIMFGAPLTVKT
                     ITDGMTLSDTYLAIAGTAAHAQILLLNGREIYTNRKGEFSEPVLLVPGYNVIEVQMTD
                     RFGKTNTKSYELVLTEQGDQYTLNTHPTALRQ"
     gene            complement(1662..>2568)
                     /locus_tag="VLB02_02875"
     CDS             complement(1662..>2568)
                     /locus_tag="VLB02_02875"
                     /inference="COORDINATES: protein motif:HMM:NF013727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HSE57001.1"
                     /translation="VELTLYNKIPHLLTPVITDPKKAILALKWAANEMNRRYDLLESQ
                     AVRDIESYHRKNQKALAAEDEAVDRMPYIVIIIDELADIMQAYPRELEAAIVRLAQMS
                     RAVGIHLILSTQRPEVNVITGLIKANIPSRIALRVPTQIDSRTILDAAGAEKLLGQGD
                     MLAIVGENQPERLQSAFITEDEVKSVVRYLADAFRDEIQDTIELTGHIGSEKTMFDSD
                     IGDGDDDELYEDARQVVMEAGKASTSYIQRKLKVGYARAARLMDMLEERGVIGPADGA
                     RPREVLDRPVKNAESDTLDTGGTPL"
BASE COUNT          577 a          635 c          635 g          721 t
ORIGIN      
        1 tactccgtat gcttgggagc gagtttacac gcctgatcac acctacgaaa atccaaacat
       61 ctacgaatgg ttcttgcagc agagtcgtta aacagtacct agagaaaaac acctcacgcg
      121 aggtgttttt cttaatgtgc agaccggtgt tattctccag agctcatcgg ctgaatttca
      181 ttgaaccgat ttttaatctc tttcaagatt tcatcgcgga gctttggatc ttctttaagg
      241 gcagtacggg tggcgtcata cccgcggcca agttttacct cgcgctcttc ttttttagag
      301 ctttcaggac gagtgtagag gtacgaagtc ccgcctggtt tggtaatgat gccaatcttt
      361 tctccgagcg ccatgatttc gccctcgagt gaaatccctt cgccgtaaat aatatcaaat
      421 tcggtttggc ggaatgggga agcgacctta ttcttcacca ctttcactcg ggtgcgtgaa
      481 ccaaccacct cttcaccttt tttaatctgg gcaatgcggc gaatgtcgat gcgtaccgag
      541 gtatagaatt taagcgcctt gccgcccggg gtagtttcag gattgccaaa catcactcct
      601 atttgcatgc gaatctggtt tataaagata actaccgttt ttgatttagc aacaatagca
      661 gtgagtttgc ggagcgcttg gctcatcagg cgcgcctgtt tgcccacgtg ctgctgtccc
      721 atctcgccct cgatttcatc ttttggggtg agtgctgcaa ccgagtcgat gacgatcacg
      781 tcaatctttc cgctgcgcac gagactttcg gtaatttcga gcccctgttc gccggtgtcg
      841 ggctgtgaaa tgaggagttc gttaatgttg actccaattt ttccggcgta gattggatcc
      901 attgcgtgct ctgcatcaat gtacgcacaa ataccgccga gcttctgtgc ttcagcaacc
      961 acgtggagcg cgagtgttgt tttaccagat gactcgggac caaatatttc aataatgcgt
     1021 ccgcggggga gcccgcccac gcccagtgcg ctgtcgagtc cgatcgaacc ggtaggaata
     1081 acatttacat caacgtgcgg ggtgtcgccc agtttcataa tcgagccttc gccgaatttt
     1141 gtttggatcg agcggatagt ttcttcaatg gaagtcttcc cggttgtttt tagttctttt
     1201 ggtacagatg gttttttagg catacaaata aagtgagtaa ttactgaata aaaaaaagaa
     1261 ctactggcga agcgcggttg ggtgtgtgtt caaggtatat tgatcgccct gttcagtgag
     1321 caccagttcg tacgatttag tgttcgtctt gccaaagcgg tcggtcattt gcacctctat
     1381 cacattatac cccggaacca ggagtactgg ttctgaaaat tcacccttgc ggttggtgta
     1441 tatttctctc ccattcaaga gtagtatttg ggcgtgcgca gcagtgcctg caatagcaag
     1501 gtaggtatcc gagagcgtca taccatcggt aattgttttt acggtgagcg gcgccccaaa
     1561 cataatatct cgggtgttgt aaaaaataat tccaatcagc gcaaagcaga gtccgagtaa
     1621 ggcaattgat tttccgtgcg gcagcggcaa gtgtcgtatc ttcatagggg agttcctcct
     1681 gtatctagtg tatcactttc tgcattcttg actggtcgat cgagcacttc acgcggtcgg
     1741 gcgccgtctg ccgggccaat taccccgcgc tcttcaagca tatccatcag gcgcgctgcc
     1801 cgagcgtagc ctactttgag ctttcgctgg atgtacgagg tagatgcctt gcctgcttcc
     1861 attaccactt gtcgagcatc ttcatagagt tcgtcatcat ccccatcacc aatgtcgctg
     1921 tcaaacatag ttttttcaga gccgatgtgt ccggtgagct caatcgtatc ttgaatctca
     1981 tcacggaagg cgtcggctaa gtaacgcact acactcttta cttcatcttc agtaataaag
     2041 gcactttgca agcgttcagg ctggttctca cccacaattg caagcatatc gccttgcccg
     2101 agcagttttt cagcgccggc agcatccaaa atagtgcgcg aatcaatttg tgttggcaca
     2161 cgcagtgcaa tacgcgaggg gatgtttgcc ttaatgagcc ctgtgatcac gttcacctcg
     2221 gggcgctggg ttgaaaggat aaggtgaata ccaactgcac gcgacatctg ggcaagccga
     2281 acaatcgctg cttcaagttc gcgcgggtac gcctgcataa tatcggcaag ttcatcgatg
     2341 atgatgacaa tgtagggcat gcggtcaacc gcttcatctt ctgcggcaag cgccttctga
     2401 ttcttgcggt ggtagctctc aatatcgcgt actgcctggc tttcaagcag atcgtagcgg
     2461 cgattcatct cattcgctgc ccacttcaat gccaagattg ccttctttgg gtcggtaatc
     2521 acgggagtaa gcaaatgtgg aattttgttg tagagggtga gctcgacc
//
LOCUS       DATIBQ010000085         3829 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U16294
            k141_37958, whole genome shotgun sequence.
ACCESSION   DATIBQ010000085 DATIBQ010000000
VERSION     DATIBQ010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935789
            Sequence Read Archive: SRR25158331
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3829)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3829)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:58:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 637
            CDSs (total)                      :: 603
            Genes (coding)                    :: 601
            CDSs (with protein)               :: 601
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3829
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37958"
                     /isolate="SMAG_U16294"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..574
                     /locus_tag="VLB02_02880"
     CDS             <1..574
                     /locus_tag="VLB02_02880"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HSE57002.1"
                     /translation="VMSFYLSVQERGIETFLRILTPVKYEKEVLSLWARTQLKIGLWF
                     QGQLLLGLIVGAITFIVLALLGVQYSFLIAVITGLFELIPFGVILAAIPAILFGVIDS
                     GLLFGLKIAIYYIIIQQIENYVLQPLIVKRVVGIPPLVVLVSFLVGISLAGFWGAILA
                     MPVAVFVLEYISDIEKSKQLVKISENPLAK"
     gene            578..2062
                     /gene="metG"
                     /locus_tag="VLB02_02885"
     CDS             578..2062
                     /gene="metG"
                     /locus_tag="VLB02_02885"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HSE57003.1"
                     /translation="MKPFYITTTLPYVNADPHVGFAMEIIRADVIARYKRLQGYEVFF
                     NTGTDEHGQKLLADATKAGKEVKEYVDHYAEQFRGLKQLLSISNEVHFIRTTDTHHEK
                     AAQELWRRVAKNGYIYKKNYEAKYCVGCELEKTDSELNEQGRCPIHPDRELEIINEEN
                     YFFALSKAASLLKKLYEEKPNLVIPDFRFNEIRAFLERGLEDFSISRLKSKMSWGVPV
                     PGDDDHVMYVWFDALTNYISTLGWPRHAEASGEGGPEDLFTKFWVTGEKVQYCGKDNV
                     RQQSMMWQAMLLAAGLPTTDHIVINGFINSGGQKMSKSLGNVISPNDIIKLLTPVAGE
                     LATDVLRYYLLRHVNSFEDSDMTIETVKEAYQANLANGLGNLVSRIMTLSEKYCDPHP
                     GEGGSNLPAEIKKTISTFDIQKAMNTIWNAIGELDGIIAATKPFSVVKEDEAEGKRMI
                     SSLVDNLFSIVHVLEPFMPNTAEQILALIKENKKPETSLFARLS"
     gene            2062..2865
                     /locus_tag="VLB02_02890"
     CDS             2062..2865
                     /locus_tag="VLB02_02890"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HSE57004.1"
                     /translation="MNFIDTHSHFNLSQFDADRDETIARMEAHDTATICVGIDAASSQ
                     FAVELAQKHQSIWAIVGQHPTEWCQEFDAEAFRQLAKQNRVVGVGECGLDYFRDRERA
                     QKEIQVKNFRAHIELAIETGLPLMLHIRPEQKTMPARLTGGDAYDDALMILKEYKQRY
                     PALTGTAHFFVGDTRVAQEFLDLGFYISFSGVITFAKEYESVVQIVSLDRILSETDAP
                     FAAPVPYRGQRSEPWQVQEVVKKIAEIKQLPLKEVQEQLLKNAQELFKI"
     gene            3331..>3829
                     /locus_tag="VLB02_02895"
     CDS             3331..>3829
                     /locus_tag="VLB02_02895"
                     /inference="COORDINATES: protein motif:HMM:NF013419.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HSE57005.1"
                     /translation="MSETENTGRVYELGYLLIPTLADGEIPEAVEGIKSLLEKNGGAV
                     FSEGTPEFIDLSYQMEHIVGSVRSKYTQAYFGWVKFEAEPHALEGIKKAVDAAKNIIR
                     SILIKTEKDNTVLFKKPKEAPRRESADQGELPLEELDEVAVEEHEKLPELANEIAAEP
                     EVAPAA"
BASE COUNT         1105 a          793 c          904 g         1027 t
ORIGIN      
        1 cgtgatgtcg ttctacctct cggtgcaaga gcgggggatt gaaaccttcc tgcgcattct
       61 tactccggtg aagtatgaaa aagaggtact ctcgctctgg gcgcgaacac agctaaaaat
      121 aggactctgg ttccaagggc agctcctcct cgggctcatt gtcggtgcta ttacctttat
      181 tgtgttggcg ctccttgggg tgcagtattc gtttctgatt gcggtaatca cggggctctt
      241 cgagcttatt ccgttcgggg tgattttggc agctattccg gcaatcttgt ttggtgttat
      301 tgatagcgga ctcctcttcg gcttgaaaat tgctatttac tacatcatta ttcaacaaat
      361 agaaaactac gtgctccagc cgctgattgt aaagcgggtg gtcggcattc cgccgctcgt
      421 ggtgcttgtt tcattcttgg ttggtatttc actcgccgga ttctggggcg cgattcttgc
      481 catgccagtt gcagtgtttg tgctcgagta catcagtgat atcgagaagt caaagcagct
      541 cgtcaaaatt tctgaaaatc cactcgcaaa ataatctatg aagccgtttt atattactac
      601 cacacttccg tacgtaaatg ccgacccgca tgttggtttt gcaatggaaa ttatccgcgc
      661 cgatgttatt gcacggtaca agcggcttca gggttatgaa gtatttttta ataccggcac
      721 tgatgagcat ggacaaaaac tacttgccga tgcaacaaaa gcagggaaag aggtgaagga
      781 atatgttgat cattacgcag agcaatttcg tggtcttaaa cagcttcttt ctatttcgaa
      841 tgaagtgcat tttatccgta ccaccgatac acaccatgaa aaagcagcac aggaattgtg
      901 gcggcgtgtt gcaaaaaatg gatatattta taaaaaaaac tacgaggcaa aatactgcgt
      961 tgggtgcgaa cttgaaaaga cagattcaga attaaatgaa caggggcgct gcccgatcca
     1021 ccctgatcga gaactagaga ttattaatga agaaaactat ttttttgcac tttcaaaagc
     1081 agcatcgctt cttaaaaaac tatatgagga aaaaccaaac ctggttattc ccgattttcg
     1141 tttcaacgag atacgagcat ttctcgagcg gggattggag gacttttcta tctcacgatt
     1201 aaaatcaaaa atgtcgtggg gagtacccgt gccaggagat gatgatcatg tcatgtatgt
     1261 ctggtttgat gcgctcacca actatatttc aaccttgggc tggcctcggc acgccgaagc
     1321 ttctggcgaa ggcggcccgg aagatttgtt taccaaattt tgggttacgg gagaaaaagt
     1381 gcagtactgc ggtaaagata atgttcgcca gcaatcgatg atgtggcagg caatgcttct
     1441 tgctgcaggg cttcctacta ccgatcatat tgttattaac ggttttatca attctggagg
     1501 gcagaagatg tctaagtcgc tcggcaatgt tattagccca aatgacatta ttaaactcct
     1561 tactccggtt gcaggagagc tcgctactga tgtgctgcgg tactatctgc ttcgccacgt
     1621 caattctttt gaagactcag atatgactat tgaaaccgtg aaggaggcat accaggcaaa
     1681 tttggcaaac ggattgggga atttagtatc gcgcatcatg acactttctg aaaaatattg
     1741 tgatcctcac cctggagaag ggggaagtaa tttaccagca gagattaaaa aaacaatttc
     1801 cacttttgat atacagaaag ccatgaatac catttggaat gcgattggag aacttgacgg
     1861 aatcattgcg gcaactaaac cattctctgt tgtaaaagaa gatgaggcgg aaggtaaacg
     1921 aatgattagt agtttagtcg ataatctttt ctcaatcgtg catgtacttg aaccgtttat
     1981 gccaaacacc gcagaacaaa tacttgctct tattaaagaa aacaaaaaac cagaaacttc
     2041 gctgtttgct cggctctcat aatgaatttt attgacaccc actcacattt taatttaagc
     2101 cagtttgatg ctgatcgtga tgaaacaatt gcacgaatgg aggcgcatga tacggctact
     2161 atttgtgtag ggattgatgc agctagttcg cagtttgcag tagagctggc gcaaaaacac
     2221 cagagtatat gggcgattgt cgggcagcat ccaaccgagt ggtgccagga atttgatgct
     2281 gaagcattta ggcagctcgc aaagcagaat cgggtagtag gggtaggcga gtgcgggctc
     2341 gactacttcc gcgatcggga acgagcgcaa aaagaaattc aagtaaaaaa ttttcgagct
     2401 cacattgagc ttgcaataga aaccgggttg ccgctcatgc tccatattcg cccagagcaa
     2461 aaaacgatgc ctgcccgcct aactggcggg gatgcgtatg atgatgcact gatgattctt
     2521 aaagagtaca aacagcggta tccagcactt actggcacag cacatttttt tgttggcgat
     2581 actcgggttg cacaagaatt tttagatctc gggttttaca tttcattttc aggggtcatt
     2641 acctttgcta aagagtatga atctgtagtt cagatagtgt cgcttgatcg gattctttca
     2701 gaaaccgatg cgccatttgc tgctccggta ccgtatcgtg gacagcggtc agagccgtgg
     2761 caagtgcaag aagtggtaaa gaaaatcgcc gagataaagc agctgccatt gaaagaggta
     2821 caagagcagc ttctaaaaaa cgcacaagaa cttttcaaga tttgacttta taattgtttc
     2881 atattatagt tttgtcgaac aaaaaaataa ctaatgaaaa agatgttgct tctactcttt
     2941 ttaagcattg tatgcttttc aacctttgct caagacacgg ctaagcctat aaaaaaagac
     3001 tctctcttga gacagaaaat tacaaatcct taccaaagat tatacaggtc tggaaatata
     3061 tattttgaaa tgtggtacat tcctggtacc ggatggattt acactaagca aggcatcgca
     3121 ccttctcttg atcggaagtg gccagttatt ctttaaagaa aacgattttt tcaaagaccc
     3181 cctaagggtc tttttgttta caaaacacta ctcttagggt agtatctctc ccgtagcccc
     3241 cgttcctcgc cggtaaggcg aggcatgctc tgctctaggg tagagaggtc cgaagcgggc
     3301 tcattagtta ataaaaagta tatattcagc atgtctgaaa cagaaaacac cgggcgggta
     3361 tacgaacttg gctacctgct cattccaaca ctcgcagatg gagaaattcc tgaagcagta
     3421 gaaggcataa agagtctcct tgaaaaaaac ggcggagctg tattttcaga gggaaccccc
     3481 gagttcattg atctgagcta ccagatggag cacatcgtgg gaagcgtacg cagtaagtac
     3541 acacaagcat actttggctg ggtaaaattt gaagccgagc ctcatgcact tgaaggtatt
     3601 aaaaaagcag ttgatgcggc aaaaaacatt atccgatcaa tcttaattaa aacagaaaaa
     3661 gataatacgg tgctgtttaa gaagcccaaa gaggctcctc gccgcgagag tgctgaccag
     3721 ggcgagctgc cgcttgaaga gcttgacgag gtagcggtcg aagaacacga aaagcttccc
     3781 gagcttgcaa atgaaattgc agcagagccc gaggttgctc cagcggcag
//
LOCUS       DATIBQ010000086         3021 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U16294
            k141_161243, whole genome shotgun sequence.
ACCESSION   DATIBQ010000086 DATIBQ010000000
VERSION     DATIBQ010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935789
            Sequence Read Archive: SRR25158331
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:58:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 637
            CDSs (total)                      :: 603
            Genes (coding)                    :: 601
            CDSs (with protein)               :: 601
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3021
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161243"
                     /isolate="SMAG_U16294"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..855
                     /locus_tag="VLB02_02900"
     CDS             <1..855
                     /locus_tag="VLB02_02900"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HSE57006.1"
                     /translation="NPMIESDYEMGSIVKPLVIAAALDMGAVTPETTYIDNTGSVQVD
                     DRVLNNFDKKGRGTASMQEVLNQSLNTGMVFVQRKMGKDAFRKYLLSYEIGDKTGIDL
                     PGEVSGIVGNLQSPGNVEYATASFGQGIATTPIAIVKAYSALANNGVMVTPHLGKRIE
                     QQGGGTKELTFKAGAQVITPETAATITNMLVKVVDKGYRRGLPNYTVAAKTGTAQVAR
                     PGGKGYYEDRNLHSLIGYFPASNPRFILYLFNYYPKGASFASETLSDPFFDTVQSLIS
                     YYELAPDR"
     gene            966..2375
                     /locus_tag="VLB02_02905"
     CDS             966..2375
                     /locus_tag="VLB02_02905"
                     /inference="COORDINATES: protein motif:HMM:NF027967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003993 - acid phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HSE57007.1"
                     /translation="MKKNYTNILFSVLGTMVMLGGFLFSALPAAAESSVDGKYEFSIQ
                     LGFGSRGDAVKALQALLATDPTLFDVQNITGYYGPKTTEAVKKFQAKHGIESVGRVGP
                     KTLRVLNTNLLSNTTLVKASTDGTSTCVMLPPGHYIAPGQLKKYGAAVLDPNCMVMLP
                     PGILKKLTGGWIGSGGSGGNQNDKTAPVISSIEVKEISTTSATITWKTDEDAVSQTLY
                     GTSNTYGSTTPKSTKYAKTHTHTITGLSANTEYHFQAKATDRAGNTATSEDKTFKTDK
                     TDSDKPEISSVTVISTTNTQANIFWVTNEPATSVVEYGTSDSYGSNVKNDTLVTLHYR
                     TLTGLEAATKYHFQVQSKDAAGNTGVSGDMTFTTASNPDSTAPIISGLTTGSITATVG
                     IVSFTTNESATATVYYSTGTPVNKGSAATVTGALNTTHAITIPDLSANTTYYFLVEVK
                     DAAGNATTATEGSFTTLVS"
     gene            2493..2756
                     /locus_tag="VLB02_02910"
     CDS             2493..2756
                     /locus_tag="VLB02_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HSE57008.1"
                     /translation="MVQEKETMIRRLKILEGQVRGLQEMVTKGAYCIDVITQTSAVKQ
                     GLSNIEDALLEHHLSHCVVAQIKTGESAKATKEIIKVYRLKRK"
     gene            2761..>3021
                     /locus_tag="VLB02_02915"
     CDS             2761..>3021
                     /locus_tag="VLB02_02915"
                     /inference="COORDINATES: protein motif:HMM:NF012621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HSE57009.1"
                     /translation="MDTKTYTIKGMHCASCAGIIEKTFKKAEGVHAAEVNYGTETAKL
                     TFDTSKTNPHRLSQKIEKLGYSLAIPASAEAMGMSASEHAAHL"
BASE COUNT          933 a          668 c          699 g          721 t
ORIGIN      
        1 aacccaatga tagagagtga ttacgaaatg ggttccatcg ttaaaccgct cgtcatagca
       61 gcagcgctcg atatgggagc ggtaaccccc gaaacaacct acatagacaa cactggttca
      121 gtgcaggttg atgaccgcgt actgaacaat ttcgataaaa agggtcgtgg cactgcaagc
      181 atgcaagagg tgctcaatca atcacttaac acggggatgg tatttgtgca gcgaaaaatg
      241 ggcaaagatg catttagaaa gtacttgctc tcgtacgaaa ttggagataa aacagggatt
      301 gatttgccgg gagaagtttc aggaatcgtg ggcaacttgc aaagtccggg caatgttgag
      361 tatgcaactg catcgttcgg acaagggatt gcgaccacgc caattgctat tgtaaaagcg
      421 tactcggcac ttgctaataa tggagtaatg gtaactccgc acttaggaaa gcgaatcgag
      481 cagcagggtg gcggtactaa agaacttacc tttaaggcag gagcgcaagt tataacaccc
      541 gaaaccgctg cgacaattac aaacatgctc gtaaaagttg ttgataaggg ttacaggcgt
      601 gggttaccca attatacggt tgctgcaaaa acagggacgg cgcaggtagc gcgcccgggc
      661 ggaaaagggt actacgaaga tcgtaatttg cactcgctga ttggttactt tccggcgagt
      721 aacccacgat tcatcctcta tttatttaat tactacccaa aaggggcttc gtttgcatcc
      781 gaaacacttt ctgatccgtt ttttgatacc gttcaatcac tcatcagcta ctacgaactc
      841 gcaccggatc gttagtggtt tgtactaaga aacaaaaata tcatttggca aggtaagcta
      901 aaatagttat aggtcgtctt actcgtgcag cagagtatag gaagctgcat ttaactatta
      961 ttattatgaa aaaaaattac acaaacatac tgttttctgt gctcggtact atggttatgc
     1021 tcggtgggtt cttattttcc gctctccctg ctgccgcaga gagcagtgtg gacgggaaat
     1081 atgaattctc aatccagctt gggttcggtt ctcgcggtga tgcggtaaag gcattgcaag
     1141 cgctcttggc gaccgatcca acactcttcg atgttcaaaa cattactggt tactatggtc
     1201 caaaaactac agaagcagta aaaaagtttc aggcaaaaca cggaatcgaa tctgttggtc
     1261 gtgttggacc aaaaacgctt cgtgtcttaa acactaactt gctcagcaac actaccttgg
     1321 taaaggcaag tactgacgga acaagtacct gtgtcatgct tccaccaggg cactacattg
     1381 caccggggca actaaagaag tatggagctg ccgttcttga tccaaactgt atggttatgc
     1441 ttccgcccgg cattcttaaa aagcttaccg gagggtggat cggcagcggt ggttccggag
     1501 gaaatcaaaa cgacaaaact gctcccgtca tcagctctat tgaagtgaaa gaaataagta
     1561 ccaccagcgc tacgatcacc tggaagactg atgaagatgc tgtttcgcag acactttacg
     1621 gtaccagcaa tacgtatggt tcaacaacac caaaaagtac gaagtatgca aagacacata
     1681 cccacactat taccgggctt tcagcaaata ctgaatatca cttccaggca aaggcgaccg
     1741 atcgggcagg gaatactgct acctcagagg ataaaacctt caagactgat aaaaccgatt
     1801 ccgataagcc agaaatttct tcagtaacag ttatcagtac caccaatact caggcaaata
     1861 tcttttgggt aacgaatgaa ccggcaacat cagttgttga atatggtacc agcgattcat
     1921 acggctcaaa tgtaaaaaat gatacgttgg taaccctgca ctacagaaca cttaccggtc
     1981 ttgaggctgc taccaagtat cacttccagg tgcagtcgaa agatgcggca gggaatacag
     2041 gtgtaagtgg cgatatgaca ttcactaccg cttcaaaccc tgatagtacc gctccaatta
     2101 tttctggact tactaccgga agtattacag caacagttgg aattgtttcg ttcaccacta
     2161 atgaatcagc aactgcaacg gtctactaca gcaccggaac tccagtaaat aaaggaagcg
     2221 ctgcgacagt aaccggagca ttgaatacta ctcatgcaat tactattcct gatctctcag
     2281 caaatacaac ctactacttc ttggtagaag taaaagatgc tgcaggaaat gcgacaactg
     2341 cgacagaagg gtcgttcacc acacttgtca gttaacttct ttgtggcatc tccgcaaata
     2401 gcttaaagtg taaaccataa aacgcctcct cagggggcgt tttatggtgg gcgagtttga
     2461 catatacccc ccagggggta tacactcgga atatggtaca agaaaaagaa acaatgattc
     2521 gtcggctaaa gatccttgag gggcaggttc gcgggttgca agagatggtt acgaagggtg
     2581 catactgtat tgatgtcatt acgcagactt cagcagtaaa gcaggggcta tcaaatattg
     2641 aagatgcgct gctcgagcat cacttaagcc actgtgtggt tgctcaaatc aaaacaggcg
     2701 agtctgcgaa agcaaccaaa gaaattatta aggtatatcg cttgaagcga aaataattat
     2761 atggatacaa aaacgtacac tataaaagga atgcactgcg cctcatgtgc gggcattatt
     2821 gaaaaaacgt tcaagaaagc agagggtgtg cacgctgctg aagtaaatta tggaactgaa
     2881 acggcaaaac ttacctttga tacctctaaa acaaatccgc atcgactttc acaaaaaata
     2941 gaaaagctcg gctatagcct cgctattccc gcatcggctg aagcgatggg catgtcggca
     3001 agcgagcatg cagcgcacct c
//
LOCUS       DATIBQ010000087         3589 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U16294
            k141_1712003, whole genome shotgun sequence.
ACCESSION   DATIBQ010000087 DATIBQ010000000
VERSION     DATIBQ010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935789
            Sequence Read Archive: SRR25158331
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:58:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 637
            CDSs (total)                      :: 603
            Genes (coding)                    :: 601
            CDSs (with protein)               :: 601
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3589
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1712003"
                     /isolate="SMAG_U16294"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(163..975)
                     /locus_tag="VLB02_02920"
     CDS             complement(163..975)
                     /locus_tag="VLB02_02920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE57010.1"
                     /translation="MEYNRKKLFFIILILLLLVAGVWRYVTLRRSPGQTGEPVTYRQF
                     FGLGGKTPAPAPGTGFQSDFTNEGEPDAEPSVQPVKNPAPQAPHSAFATPALTPTSTA
                     QTAAALGNNYFGTSSGTPGRSTRGTGSSLNGNLDLEDDLLDPDTLLCDPRDITIEFTP
                     AELDRLNQLEQQLAQLAPTLRSAENVEEEIANYENFKIYNNRIVEYKNFCFNKTAQFT
                     TKPARRATPFWSDSTLNTNKFYPVNKAFSYAKGDNDIKSFYFIENYFNMNIW"
     gene            complement(977..3088)
                     /locus_tag="VLB02_02925"
     CDS             complement(977..3088)
                     /locus_tag="VLB02_02925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE57011.1"
                     /translation="MKFRFFLLSITLGTLALGSHVQAQTNVNTGDIGTAVSATIACTN
                     VGGLVVGGLNDLVNGSGKSGDKGKQLGIGDSSQGSQKVPTDDEKAQKKLEDQNFKEKC
                     LDKIAYGVSRSALATQVAKTLNWVNKGAGGNPLYILNQNSYFKSIGEKQMKKYLEQTP
                     LRDGVFGETIRNTIISQATGKPLPGGGNSAGSSLFSNFFSTTKNKALDDFNKDFRNGG
                     WDMWQRTVDGDLNPIVAYINETGVIGKNIQETIDTVKAELQQGSGFMPQRKCAQYEEK
                     PSMTDPNSTNYDPSYKPECLQWKTITPGAIVADQAKDALGSPMRQLELADELNEVLGS
                     LFGKIMNKLLTKGVDGLGSFASGDYSAFGDVGVNNLKGKVSNTIEIDTASGAGNVNVK
                     NPGSVRDVIKNQYNFLSATHDSQRAMEQIAPNLGILDYCLPGPHQTWKEEFEGNMVPF
                     VETVINAPESGRFRDVKEVTLKDFVTGKLFTKSGFGIKVESGISNVLQGITAFLNVQP
                     MGGSGAAITNLFVKQTDTSGVAAQFVKFFGMYEADVEPLYTTAGVVNQFTAREPEALK
                     TSTANMIRAALTETRDLVEVYDNSSEMLIAYRDADTQTKEHIRNLERIYAEAREIVEK
                     ARIRHIAKMKAEGYTVNMACLNAVYDINPAYATRKPVEPDASNPIVEQVKKKHQEFYS
                     NIGDRSPGNGTIEQFVEVKLE"
     gene            complement(3088..>3589)
                     /locus_tag="VLB02_02930"
     CDS             complement(3088..>3589)
                     /locus_tag="VLB02_02930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE57012.1"
                     /translation="DAATLQKIAADIGADINTNGLVNRYRLADIKTVQTTQASLEAYH
                     AAFSTIYNKYSTIGTELTPIAAAADTGVIEDPEGLRTIAVAYQSLAQKIAAVRAPIGV
                     AAYHLAIINELDHIGKSIELILALEDGGINAMIGASFYGNYTLALANSSRALKEYLTE
                     YSILDS"
BASE COUNT          810 a          809 c          881 g         1089 t
ORIGIN      
        1 agtagtaccc tgcgcatata ctgccggctg ctttccaaac acgccttgga gcggaccaat
       61 gagtaacgct actccaataa aaaagagcgt gagttttgta aacagggtgg tgcagcgacc
      121 agacgcttta gagaaaagca taaatatgct gcaagtatac cattaccaga tgttcatatt
      181 gaagtaattt tcaatgaagt agaagctttt tatgtcgtta tctccttttg cataactaaa
      241 tgccttattt accggataga acttgttggt gttgagtgtt gagtcgctcc aaaacggtgt
      301 agcacggcgc gcaggttttg tagtaaattg agcagtttta ttgaagcaga agtttttgta
      361 ctcgacaatg cggttattgt agattttaaa gttttcgtag ttcgcgattt cttcttcaac
      421 gttctctgcc gagcgcaagg tgggggcaag ctgggcaagc tgctgctcga gctggtttaa
      481 gcgatcaagt tctgcagggg taaattcgat agtgatatca cgtggatcgc agagcagggt
      541 gtcggggtcg agtaaatcgt cttcaagatc gaggttgcca ttgaggcttg aaccagtgcc
      601 ccgggtactg cgccccggtg tccccgagga ggttccaaag tagttatttc ctaatgcggc
      661 tgctgtttga gcagtactgg taggggtaag tgccggtgtg gcaaatgcgg agtgcggcgc
      721 ttgaggggca gggtttttta ctggctggac gcttggttcg gcgtctggtt caccttcatt
      781 cgtaaaatca gattggaagc cggtaccggg ggcaggagca ggggtcttac ctcctaagcc
      841 aaaaaactgg cggtaggtta ctggctcccc tgtttgcccg ggcgagcgtc tgagtgttac
      901 ataacgccat acacctgcaa caagcaacag cagtataagg atgatgaaaa atagtttttt
      961 tcgattatat tccatattat tctagtttta cctctacaaa ctgctcaatg gttccattgc
     1021 cggggcttct atcgccaata ttgctataga attcttggtg tttctttttt acctgctcaa
     1081 caatggggtt gcttgcgtct ggctcgaccg gctttcgagt tgcatacgca gggttaatgt
     1141 cgtataccgc attcaagcat gccatattta cggtgtatcc ttccgccttc atttttgcga
     1201 tgtggcgaat acgagctttc tcaacaattt cacgtgcttc tgcataaata cgctcgaggt
     1261 tgcgtatgtg ttcttttgtt tgggtatctg catcgcgata cgcaataagc atttcagagc
     1321 tgttgtcgta cacctcgact aaatcccgtg tttcagtcag tgccgcgcgg atcatgtttg
     1381 cagtacttgt tttgagtgct tcaggttcgc gggcagtaaa ttgattcacc acacctgcgg
     1441 tagtgtagag tggctcaaca tcagcttcat acatgccaaa gaattttaca aactgtgctg
     1501 cgacacccga agtatcggtt tgctttacaa acaagttagt aatagctgcg cccgagccgc
     1561 ccatgggctg tacgttcaag aaggcagtaa tcccctggag tacatttgaa attccgcttt
     1621 ccactttaat accaaaccct gatttagtga ataactttcc ggtaacgaaa tcttttaacg
     1681 ttacctcttt tacatcgcga aatctgccgc tctcaggtgc attgataact gtttcaacaa
     1741 agggaaccat atttccttca aattcttcct tccaggtttg gtgcggtccg gggaggcagt
     1801 agtcaagaat gccaagattt ggcgcaatct gctccatcgc ccgctggctg tcgtgggtgg
     1861 cgctcaaaaa attgtactga ttcttgataa cgtctcgcac tgagccgggg tttttcacat
     1921 tcacattccc tgcaccagag gcagtatcaa tttctatcgt atttgaaact tttcctttta
     1981 agttatttac cccaacatcc ccaaacgcac tgtagtcgcc gctcgcgaat gaccctaaac
     2041 cgtcaacacc tttcgtcaga agcttgttca ttatttttcc gaatagagaa cctaacacct
     2101 catttaattc atcggcgagc tcaagttggc gcatcgggct accgagtgca tcttttgcct
     2161 ggtcggcaac aattgctccg ggggtaattg ttttccattg tagacactct ggtttatagc
     2221 tcgggtcgta gttggtacta tttggatcgg tcattgaagg tttttcttca tattgagcgc
     2281 attttcgctg cggcataaac ccagaacctt gctgaagttc agctttcacg gtatcaattg
     2341 tttcttgaat gttttttcca ataacgccgg tttcgtttat gtaggcaacg attgggttta
     2401 agtcgccgtc aacggtccgc tgccacatgt cccagccgcc gtttctaaaa tctttgttaa
     2461 aatcgtcgag cgctttgttt ttggtggtgc taaaaaagtt actgaacagg ctgcttcctg
     2521 cactgtttcc cccgccgggg agcggtttgc cagttgcctg gctgataatg gtgttgcgga
     2581 ttgtttctcc gaatacgccg tcacggagcg gagtctgctc gaggtatttc ttcatctgct
     2641 tttcaccgat cgatttgaag tacgagtttt ggttgagaat gtagagcgga tttccgccgg
     2701 cacctttatt tacccagttg agcgtttttg ctacctgggt ggcaagcgcc gatcgagaga
     2761 cgccataggc gattttgtcg agacattttt ctttaaagtt ttgatcttct agcttttttt
     2821 gcgctttttc gtcgtcggta ggaacttttt gactaccctg tgatgagtct ccaataccga
     2881 gctgttttcc tttatctcca ctcttccctg aaccatttac cagatcattt aatcctccta
     2941 ccaccagtcc acccacattg gtacaagcaa tggtagctga aactgcggta ccgatgtcgc
     3001 cggtgtttac atttgtctga gcttgtacat gagaaccaag cgcgagtgta ccaagggtga
     3061 tggagagtaa gaaaaagcga aacttcatta cgaatcaagt atactatatt cagttaaata
     3121 ctctttcagt gcgcgtgaag agttggcaag tgcgagcgtg tagtttccgt aaaaacttgc
     3181 gccgatcatt gcgttgatcc cgccgtcttc tagggcaagg atgagttcga ttgattttcc
     3241 gatatggtcg agttcgttga taattgccag atggtatgcg gcaacgccaa tgggcgctcg
     3301 caccgctgct attttttgtg cgagcgactg gtaggcaaca gcaatggtac ggagcccttc
     3361 tgggtcttca ataacgccgg tgtcggcagc agcagcaatc ggtgtgagtt cggtgccgat
     3421 agttgaatac ttattatata tagtagagaa cgccgcgtgg tacgcttcga gtgaggcttg
     3481 ggttgtttgt accgttttta tgtcggcgag ccggtatcgg tttactaacc cattcgtgtt
     3541 tatgtctgcg ccgatatctg cagctatttt ctgcagtgtt gcagcatca
//
LOCUS       DATIBQ010000088         2855 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U16294
            k141_42972, whole genome shotgun sequence.
ACCESSION   DATIBQ010000088 DATIBQ010000000
VERSION     DATIBQ010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935789
            Sequence Read Archive: SRR25158331
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:58:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 637
            CDSs (total)                      :: 603
            Genes (coding)                    :: 601
            CDSs (with protein)               :: 601
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2855
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42972"
                     /isolate="SMAG_U16294"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..377
                     /gene="ssb"
                     /locus_tag="VLB02_02935"
     CDS             <1..377
                     /gene="ssb"
                     /locus_tag="VLB02_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HSE57013.1"
                     /translation="VTSFSLATNRTWKDQNGAKQEAVEYHNIVVFGKQAEIIKQYCKK
                     GSSLYVEGRIQTRSWDAQDGTKKYRTEIVLENFQFGPKPGFGGGAPEQGSQASAKSPA
                     AEEQLDTIEYPNEEINLEDIPF"
     gene            381..578
                     /gene="rpsR"
                     /locus_tag="VLB02_02940"
     CDS             381..578
                     /gene="rpsR"
                     /locus_tag="VLB02_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HSE57014.1"
                     /translation="MKNDFFNVNNIRFVDYKDLENLKRFLNPNGRILSRKRTGLTAKN
                     QRAVAEAIKRSRFLGLLPYIA"
     gene            659..1165
                     /locus_tag="VLB02_02945"
     CDS             659..1165
                     /locus_tag="VLB02_02945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE57015.1"
                     /translation="MKKAIINLFCVVFLVSCIAPKKHSTENPNGYYKGEMIRVLKTDP
                     DFEKIKIAYERLSSFSSTVKGTWSRFGEQYLITQTINGVVFQVHVDNSDIRATFSKSV
                     SFKFNRDFQTEDVVAFEWFTTNDNAKTLTREKLIAYIIAAGNAVLFPKINLKTFHQQH
                     GGGFDFLV"
     gene            complement(1446..2030)
                     /locus_tag="VLB02_02950"
     CDS             complement(1446..2030)
                     /locus_tag="VLB02_02950"
                     /inference="COORDINATES: protein motif:HMM:NF020845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE57016.1"
                     /translation="MALLEYSEVRERKIILWEGEPYEVISSHVFRKQQRKPVNATKLK
                     NLVSGRVAEVSFASSDKIEEADIATRPVKFLYKNRGEAWFADPKDPSKRFSLKEEIIG
                     DRLRFVKENTGLDLTIFTNEDDEEKIIGIKVPVKVELKITEAPPSIKGSSVTGGTKIV
                     TLETGATINVPPFLNEGEVIRINTETGEYVERAN"
     gene            2135..>2855
                     /locus_tag="VLB02_02955"
     CDS             2135..>2855
                     /locus_tag="VLB02_02955"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HSE57017.1"
                     /translation="MKQGTQQKGRAGFTLIEVIVVLAILALFSAIVVFAVGLSRAKGE
                     DARTKETLASVRTSATDEYIELASFNTVCSSGKTHTIITTLAAQQGLSITDYICVADP
                     TQFAVLFPLKAESGYWCVDSTGNAKRMATADDPLSSKSCGPPPPPAEEGGGGGTEEEG
                     GPSNLPPIASAGADQNITLPTNSVVLDGSGSDFDGSITSYLWTKLSGTGGTIQSPLLP
                     DTNITGLSAGTYVFQLAVTDNG"
BASE COUNT          801 a          593 c          655 g          806 t
ORIGIN      
        1 aagtaaccag tttttcgctt gcaacaaatc gcacctggaa ggatcagaac ggcgcaaagc
       61 aagaggcagt tgaatatcac aacattgtgg tgttcggcaa gcaggctgaa attattaagc
      121 agtactgcaa aaaaggaagc tcgctctatg tagaggggcg cattcaaacg cgcagctggg
      181 atgcacagga cggcaccaaa aagtaccgca ctgaaattgt gcttgaaaac ttccagttcg
      241 gaccaaaacc gggatttggc ggcggcgcac cagaacaggg aagccaggca tcggcaaaat
      301 caccagcagc cgaagagcag ctcgatacga ttgagtaccc gaacgaagaa attaaccttg
      361 aagatattcc attctaattt atgaaaaacg atttttttaa cgtaaacaat attcgctttg
      421 tcgactacaa agatttagaa aacctcaagc ggtttttaaa tccgaacggt cgcattctct
      481 cgcgcaagcg cacggggctt actgccaaaa accagcgcgc cgttgcagaa gcaatcaagc
      541 gttctcgatt tttgggactc ttgccttata tcgcttaaat agaaaagcaa acaccgccct
      601 tgtgggcggt ttttgctttt gttatatggg agtggtaagg tgctaaaaaa cttttagtat
      661 gaaaaaagcc attatcaacc tattttgtgt agtattcctt gtcagttgca tagcaccaaa
      721 aaagcatagt actgaaaatc caaacgggta ttacaaaggg gaaatgattc gagttcttaa
      781 aacagacccc gatttcgaga aaataaaaat cgcctatgaa agactttcaa gtttttcatc
      841 aactgtaaaa ggtacttggt ctcgttttgg agagcaatac cttatcaccc aaacaatcaa
      901 tggtgtagtg tttcaggtac atgtagataa tagtgatatt cgtgcaacat tctcgaaatc
      961 agtatcattt aaatttaatc gtgattttca aacagaagac gttgttgcgt ttgaatggtt
     1021 tactaccaac gataacgcta aaacattaac ccgagagaaa ttgatcgcgt atattattgc
     1081 agcaggaaat gcagtactgt ttcctaaaat taatttaaaa acattccatc aacaacacgg
     1141 tgggggtttt gattttttag tataggagtg ttactgtttt caaaaatgtt cttctatgga
     1201 aaagaaagtt agcagcagaa ttgctattat tatttccgtg agtcctttta cgtgccttgg
     1261 ttttatgatt ggagccatta caacacgaat agccggaaca tcgtggtggt ggacagtcgg
     1321 ttgcgcattc ttctttgttg gatcactcgt tatatttaga aaatgtgcaa ataaactcta
     1381 aatattaaaa atccgccctt cgtgtatgtt cgggacggat tttttttata cactataatt
     1441 aaaatttagt ttgctctttc tacgtactcg ccagtctcgg tattgatgcg gataacttct
     1501 ccctcgttga ggaacggggg aacgttgata gttgcgccag tttcaagagt aactattttt
     1561 gtgccaccgg taacactact gcctttaatt gaaggcggtg cttcggtaat ttttaattca
     1621 actttgaccg gcactttaat accaataatt ttttcctcgt cgtcttcgtt ggtaaatatc
     1681 gtaaggtcga gcccggtgtt ttcttttaca aacctcaatc tatcaccaat aatttcttct
     1741 tttagtgaga agcgcttgct tgggtcttta gggtcggcaa accatgcctc gccgcggttt
     1801 ttatacaaaa attttacggg gcgggtagca atgtcggctt cttcaatttt atctgacgat
     1861 gcaaatgata cctcggcaac tctaccgctc actaaatttt tgagtttggt tgcatttacc
     1921 ggcttgcgct gttgctttcg aaacacgtgc gaagaaataa cttcgtacgg ttcgccctcc
     1981 cagagtatta ttttccgctc ccggacttcg ctgtattcaa gtagtgccat aatagttttt
     2041 ctaaaaaata agtaccagcc cattatacgg acggagggca caaacgcaag gcgtgctata
     2101 attttcttac tctttaatca ttacagagtg cagtatgaag caaggtacac aacagaaggg
     2161 tcgggcaggg tttaccctga tagaggtaat tgtggtgctc gcgattcttg cgctgtttag
     2221 tgctattgtt gtgtttgcgg tcggtctctc gcgggcaaaa ggtgaggatg ctcgaaccaa
     2281 agaaacactg gcaagtgtcc gcacgagtgc aactgacgaa tatattgaac ttgcctcgtt
     2341 taacacggtg tgcagctcag gaaaaacaca taccatcatt actacgcttg ctgcgcagca
     2401 ggggctttca attactgatt acatttgtgt tgctgatcca actcagtttg ccgtactctt
     2461 tccgctcaag gcagaaagcg ggtactggtg tgttgactca actggcaacg caaaacgtat
     2521 ggcaacggca gatgatccac tgagttcaaa atcgtgtggt ccaccacctc ctcctgcaga
     2581 agagggtggc ggtggaggga cagaggaaga ggggggacca agcaatcttc cgccgattgc
     2641 aagtgcgggt gctgatcaga atattacatt gccaaccaat tcagtggtgc tcgatgggag
     2701 cggttcggat ttcgacggga gtattacctc gtatctgtgg acgaaacttt caggaacggg
     2761 aggaacaatt caatcaccgc tcttgcccga tacaaatatt acggggctct cggcggggac
     2821 gtacgtgttt cagctcgcgg tgactgataa cggcg
//
LOCUS       DATIBQ010000089         5433 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U16294
            k141_1392462, whole genome shotgun sequence.
ACCESSION   DATIBQ010000089 DATIBQ010000000
VERSION     DATIBQ010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935789
            Sequence Read Archive: SRR25158331
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 5433)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5433)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:58:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 637
            CDSs (total)                      :: 603
            Genes (coding)                    :: 601
            CDSs (with protein)               :: 601
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5433
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1392462"
                     /isolate="SMAG_U16294"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1064
                     /locus_tag="VLB02_02960"
     CDS             <1..1064
                     /locus_tag="VLB02_02960"
                     /inference="COORDINATES: protein motif:HMM:NF014516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE57018.1"
                     /translation="LELEEVEREMKKLTRRQEAVEGELAEARKHREAHLRAREGASEA
                     RFQIRSDIQRCETELQMLSVTMAQIDTRRSYFDTEIKEAGSLFGPSFLHALHTATVLP
                     QESLADLKRSVDRLKIRYEEHSGIGNEVIKEYDDTKERDAFLTREMADIEAGIANLQM
                     LIIDLKKTLDDRFSNGVQSINKHFGEFFKTMFGGGSAFLSITMEKKRKRKKIEDSESD
                     EEYEDPELAEGEENQFLERGIDIHVSLPEKKVKELTMLSGGERSLVSIALLFAISQVN
                     PPPFLVLDETDAALDEANSRRYGDMIEKLAEFSQLIVVTHNRETMSRASVLYGVTMGA
                     DGSSKLLSIKFDEAVKVAK"
     gene            1081..1410
                     /locus_tag="VLB02_02965"
     CDS             1081..1410
                     /locus_tag="VLB02_02965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE57019.1"
                     /translation="MEGNNFEKVNSNKENAEVIEIKFALDNEVQKENPENWVRVIKWI
                     AEAFTKGLQAEDKEAIKEAYSQIKTHDNYLSAKYRSTDEQQVYLRHVQAAMDYLSDRL
                     LSGPTRQ"
     gene            1464..1685
                     /locus_tag="VLB02_02970"
     CDS             1464..1685
                     /locus_tag="VLB02_02970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE57020.1"
                     /translation="MATTKRRLNITLDPALEIAIAKSAKRDRVPEATKAAELLSFALS
                     LEEDSGLEALAKSRDIKGANMIKHTAAWK"
     gene            1690..1962
                     /locus_tag="VLB02_02975"
     CDS             1690..1962
                     /locus_tag="VLB02_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006964744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HSE57021.1"
                     /translation="MYEIFYHKLVVTADIPKLGTVERKRIKKAIELKLTTHPELYGVP
                     LHQSLAGYRKLRVGDYRVVFRIAKKQVLVFIIGHRSMVYEVIEKRI"
     gene            2033..2551
                     /locus_tag="VLB02_02980"
     CDS             2033..2551
                     /locus_tag="VLB02_02980"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HSE57022.1"
                     /translation="MKKATCVYCVNEATNQVLMAWQQKKIVGLKGYGGVFDPDAGDIT
                     MEDCAVREFKEESGLDIFPEHLELFARVVFYNGTVEEVPEENPSFEVWFYRYRHPLQG
                     TPVSTPEMQNPDWFPMGEALPLADMIQGDEYFIPSVLAGTPTSGRIRRTSDWKKILEK
                     QFECCTPQDLAA"
     gene            2623..2928
                     /locus_tag="VLB02_02985"
     CDS             2623..2928
                     /locus_tag="VLB02_02985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE57023.1"
                     /translation="MDTGIVPLTKESSSILCQCCSVPAVGVDSEGKRTCNIPGNHEGN
                     EMVGPIFVKEVPPHTMCTICGKSATLYAGGDTYLCSAAAHEGYDETTLIPLVPTTAE"
     gene            complement(2956..4236)
                     /gene="murF"
                     /locus_tag="VLB02_02990"
     CDS             complement(2956..4236)
                     /gene="murF"
                     /locus_tag="VLB02_02990"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HSE57024.1"
                     /translation="MDSKTLYNTICKAPFSISTDTRTLQEGQCFFCLRGETFDGNTFA
                     EAALEKGAQLVVVDNPAAVLSEKCMLVENTLKALQELAQIHRSHFSIPVIAIGGSNGK
                     TTTKELVAAVLGTEKKVHVTKGNLNNEIGVPLTLLALQPEAEIAIIEVGANHSGEHTK
                     LMEIVSPTHILVTNNGADHLEGFGSLAGVRAANKELFDWARTHSAHAFVNKALPDLVA
                     DSAGSSRMLYPTAPYKSTSHMLASLEYKGTPIHSNLFGSFNEANILAALAIGEHFAIS
                     LENMQKAITTYQPTLHRSQIYKGENYVAVLDYYNANPTSMQLALTDFIQSTPAGKRIL
                     ILGDMLELGTAEQAAHEGVLSFIKTAADPKDTIFCIGPRFKQYAPQYPFHFFETATKA
                     RPEFNIIELAGTYLFAKGSRGIKVEEVIEEKILF"
     gene            4294..4791
                     /locus_tag="VLB02_02995"
     CDS             4294..4791
                     /locus_tag="VLB02_02995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE57025.1"
                     /translation="MIYAVNSIDIAYLERYLSKDTYISFACHQQYTYFATFTHGTGEE
                     RTVVSYKCFAHLCLLLGSEITGDSSVKDRANDRYPDSLAIGQPKQFDRFLINGAQVWM
                     SKPSENTIRLDSLSIERISRLPGLCQRVGFGDSFTEAVEELFQSDPVKVNSSIFWNIL
                     PKQLV"
     gene            4920..5210
                     /locus_tag="VLB02_03000"
     CDS             4920..5210
                     /locus_tag="VLB02_03000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE57026.1"
                     /translation="MENKIKLGVQMNSSGDSLKMQMETPVKTAVICTLFNSSKAVIGL
                     LRWQLVEGVNVANITKPILEGAPPVKFFPDTYHLVVTSEFGEFLYKKKLIKE"
BASE COUNT         1581 a         1062 c         1287 g         1503 t
ORIGIN      
        1 ctcttgaact tgaagaagtt gaacgcgaga tgaaaaagct cacccggcgg caagaagcag
       61 ttgagggtga gctcgcagaa gcacgaaagc accgcgaggc gcatctgcgg gcacgtgagg
      121 gggcgagcga ggcgcgattt cagattcgct ctgacattca gcgatgcgaa acggaacttc
      181 agatgctctc ggttacgatg gcacagatcg atacccgccg tagctatttt gatactgaaa
      241 taaaagaagc cggatccttg ttcggtccaa gttttttgca tgcgctgcat actgcaacgg
      301 tgctgcccca agaatcgctc gctgacctca aacgttcggt tgatcgccta aaaatccgct
      361 acgaagagca cagcgggata gggaacgagg tgattaagga gtatgacgac accaaagagc
      421 gtgatgcatt tttgactcgt gaaatggcag atatcgaggc gggcattgca aacttgcaga
      481 tgctcattat tgatttaaag aaaacgctcg atgatcggtt ctcaaacggg gtgcagtcga
      541 tcaataaaca ctttggtgaa tttttcaaaa caatgtttgg cggcggctcc gccttcctct
      601 ctattaccat ggagaaaaag cggaaacgga agaaaatcga ggattccgag tctgacgagg
      661 aatacgaaga tcccgagctc gccgagggag aagagaatca atttttggaa cgtggtatcg
      721 atattcatgt ttcgctccct gagaaaaaag taaaagagct cacgatgcta tcgggtggcg
      781 aacgctcact tgtgtctatt gcacttttgt ttgcaatttc gcaggtaaac ccgccgccgt
      841 ttttagtgct cgacgaaact gatgcagcac ttgatgaagc caacagccgc cgctacggcg
      901 acatgattga gaaacttgcc gagttttcgc agttgattgt ggtaacccac aatcgcgaaa
      961 cgatgagccg ggcaagtgtc ctctatggcg ttactatggg tgccgacggc tcctcaaaac
     1021 tcctctctat taagttcgat gaagcagtga aggttgcaaa gtaagattta taatagggtt
     1081 atggaaggaa ataattttga aaaagtaaat tcgaataaag aaaacgcaga agtaattgag
     1141 attaaatttg ccttggacaa tgaagtgcaa aaagaaaatc cagaaaattg ggtgcgggtc
     1201 attaaatgga ttgctgaagc tttcacaaaa ggattacagg cagaagataa agaagctatc
     1261 aaggaagcat atagtcaaat aaaaactcac gacaattatt taagtgcaaa atatcgttct
     1321 accgatgaac aacaggtata tcttaggcat gtgcaagctg caatggacta tctttctgat
     1381 cgtcttttat caggtccgac gaggcagtga aagtggcgaa atagggtttg acaatggtga
     1441 tacacttttg atacacttga cgtatggcta ctacaaaacg gcgattaaat attacccttg
     1501 atccggcgct tgaaattgca attgccaaaa gcgcaaagcg cgatcgggtg cctgaggcaa
     1561 ccaaagcagc agaactgctc tcttttgcac tgtctcttga agaggatagc gggcttgaag
     1621 cgcttgcaaa atcacgagat ataaaggggg cgaatatgat caaacacaca gctgcgtgga
     1681 agtaattgta tgtatgagat tttctaccac aaactagtgg taacggctga tattccaaaa
     1741 cttggtacgg tcgagcgtaa acgaatcaaa aaagcgattg agctgaaatt aactactcac
     1801 ccagaactgt acggagtgcc actccaccag agtcttgcgg ggtaccgtaa gcttcgtgtc
     1861 ggtgattatc gggttgtttt tcgtattgcc aaaaaacagg tacttgtttt tatcatcgga
     1921 caccgatcaa tggtgtacga ggttattgaa aaaagaatat agtgtggttt cgacaaagcg
     1981 gtgaaagttg caaaataaga aggggagagt accatcgggt aaaatagtaa ctatgaaaaa
     2041 agcaacttgc gtctattgtg ttaatgaggc aacgaaccag gtgctcatgg cttggcagca
     2101 gaaaaaaatt gttggactca aaggatatgg cggtgtgttt gatcctgatg cgggagatat
     2161 aaccatggaa gactgtgcag tgcgagagtt taaagaagag tcggggttag atatatttcc
     2221 tgagcatctt gaactctttg ctcgagtagt gttttacaac ggaacagtag aagaagttcc
     2281 agaagaaaat ccttcgtttg aagtgtggtt ctatcgctac cgacatccac tgcaaggaac
     2341 gccagtttca actcctgaaa tgcaaaaccc ggactggttt ccgatgggag aagcacttcc
     2401 acttgccgac atgattcaag gagatgagta ctttattcca tcggtacttg ccggcactcc
     2461 taccagcggt cgaatccgtc gaaccagtga ctggaaaaag atcctcgaaa aacaatttga
     2521 atgttgcact cctcaagacc ttgctgcgta gctatgagca aggtctttta cttttccatt
     2581 tctcttaaaa acctgctata acctaaagaa aaaggaggat atatggatac cggcattgta
     2641 cccctaacaa aagaatcttc ttctatacta tgtcagtgct gctcagtgcc agcagttggc
     2701 gtagacagcg agggaaagcg gacatgcaac attcctggaa accacgaagg gaatgagatg
     2761 gttggtccta tttttgtaaa agaagttcca cctcatacta tgtgtactat ttgtggtaag
     2821 tctgcaactc tatacgcagg tggagatact tatttatgta gcgctgctgc ccatgaaggg
     2881 tatgacgaaa caactctcat tccgctggtt ccaacaactg cagaatgaat ttaaatcctg
     2941 ttttaataca tgtaattaaa acaggatttt ttcttctatc acctcttcaa cttttatgcc
     3001 gcgagagcct ttggcaaaaa ggtatgttcc agcaagttct ataatgttaa attcagggcg
     3061 ggctttggtg gcagtttcaa aaaaatgaaa ggggtactgt ggtgcatact gtttgaaccg
     3121 cggaccgatg caaaaaatag tatcttttgg gtctgcagct gttttaataa aagagagtac
     3181 tccttcatgg gctgcttgtt ctgcggtgcc aagctcgagc atgtcgccca aaatcagtat
     3241 ccgtttgccg gcaggagtac tctggataaa atccgtaaga gcgagctgca tactggtggg
     3301 gttagcattg tagtaatcta aaacagcaac atagttttcg cctttgtata tttgagagcg
     3361 atgcagggta ggctggtaag tggtaattgc tttttgcatg ttttcaagcg aaatagcaaa
     3421 atgctcacca atagcaagtg cggcaaggat gtttgcttca ttgaatgacc caaacaagtt
     3481 tgagtgaatg ggtgtccctt tgtactcgag gctagcgagc atgtgcgaag tgcttttgta
     3541 cggggcagtt gggtacagca ttcggctgct gccagcgcta tctgctacta aatcgggcaa
     3601 tgctttgttt acaaacgcgt gcgcactgtg cgttcgtgcc cagtcaaata attctttgtt
     3661 tgccgcgcga acgcctgcaa gcgatccaaa cccttctaga tgatcggcgc cgttgttggt
     3721 aacgagtata tgggtagggg aaacaatttc cataagtttt gtgtgttcac cagagtgatt
     3781 tgcaccaact tcaataatgg caatttcggc ttcaggctgc agtgccaaca gggtaagggg
     3841 aacgccgatt tcgttattta aattgccttt tgttacatga accttctttt ctgtaccgag
     3901 cactgcggca acaagctctt tggtagttgt ctttccattc gagccgccaa tagcaataac
     3961 aggaatagag aagtgtgaac ggtgtatctg cgcaagctct tgcagtgcct taagtgtatt
     4021 ttctaccaac atgcattttt cgcttagaac cgcggcagga ttatcaacaa caactagctg
     4081 agcgcctttt tctaaggcag cctccgcaaa ggtgttccca tcaaatgttt ctcctcgtaa
     4141 gcaaaaaaaa cattgccctt cctgcaaagt tctggtgtcg gtagaaatag aaaatggtgc
     4201 tttgcagatg gtattgtaca gtgttttact atccataccg tgtagactat caataaatgc
     4261 aattttaggg tagtatcgtt aaaaatcttt tttatgatat acgctgtaaa tagtatcgat
     4321 atcgcatatt tagagaggta tctttctaag gatacctaca tcagctttgc ctgccaccag
     4381 caatatactt actttgcgac ttttactcat ggcacaggag aagagcggac agtggtatcg
     4441 tacaagtgtt ttgcgcacct ctgcctgtta cttgggtctg aaattaccgg agattcttct
     4501 gtaaaggaca gggcaaatga tcggtaccca gattcgcttg ctattgggca accaaagcag
     4561 tttgatcgct ttcttataaa cggagcgcag gtttggatgt ccaaaccgtc tgaaaatact
     4621 attcgtttag attctctgag tatagaacga atttctcggt tgccaggttt atgccagcga
     4681 gtaggatttg gggactcttt tactgaagca gtagaagagt tgtttcagtc agatccggtt
     4741 aaggtaaact cctctatttt ttggaatata ctaccgaaac aactagttta atgttctact
     4801 aacagccccc gcagtacacg cgggggattt ttttaccgag tcattgactt ttaatttaaa
     4861 ttatatataa tgtttcttaa gtttttcttt atatttaacc aaaaaccatt acgattacga
     4921 tggaaaacaa gattaaacta ggtgttcaga tgaattcctc tggagattca ctgaaaatgc
     4981 aaatggaaac cccggtaaag acagcggtta tctgtacttt atttaattca agcaaggcgg
     5041 taatcggatt attgcgttgg caacttgtgg aaggggtaaa tgtagcgaat attactaaac
     5101 ctatactaga aggtgcaccg ccagttaaat tttttcctga cacctatcat ctggtggtca
     5161 ctagtgaatt tggtgagttt ctttataaga aaaagctcat aaaagagtaa attaaataag
     5221 ttttaaccat tatccccttg aaacagggga ttttcttatg aacaagagaa gtattgactt
     5281 ccggtaaaat gtatttatag ttatagccga ggatattttt tgaaaactta taaatttttt
     5341 ctgatgaaaa aaaatgcttt tttcctactt gttagtatat gtctggcaag tggtgtgatg
     5401 gcacagttgt tagtgcctga ctcgatatcg ata
//
LOCUS       DATIBQ010000090         7275 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U16294
            k141_383963, whole genome shotgun sequence.
ACCESSION   DATIBQ010000090 DATIBQ010000000
VERSION     DATIBQ010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935789
            Sequence Read Archive: SRR25158331
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 7275)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7275)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:58:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 637
            CDSs (total)                      :: 603
            Genes (coding)                    :: 601
            CDSs (with protein)               :: 601
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7275
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_383963"
                     /isolate="SMAG_U16294"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            193..996
                     /locus_tag="VLB02_03005"
     CDS             193..996
                     /locus_tag="VLB02_03005"
                     /inference="COORDINATES: protein motif:HMM:NF025112.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HSE57027.1"
                     /translation="MLQKTGLLTSVAPEFLEGVGCEQKGAHIYDVFEHLLHALQHAAD
                     KGFSTEVRLAALFHDIGKPATRRYDRTKDKYTFFGHEVVGARMTKKILERLKFSRETV
                     SRVTTLVRNHMFFSDTEKITLSAVRRIIRNVGAEQIWLLMEVRECDRVGMKKKEAPYR
                     LRKYHAMIEEALRDPITVGQLAIDGTYLMETLHMKPGPRMGWILHALLEEVLENPKLN
                     TIEQLKERVLDLNELEDKDLKTLGEKGKERKEEANEGELEELRKKHGVK"
     gene            1165..1434
                     /locus_tag="VLB02_03010"
     CDS             1165..1434
                     /locus_tag="VLB02_03010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE57028.1"
                     /translation="MGNRSYSMQKLITVLFLCFLLEITITEPTLLAVSGIILFEVPIN
                     AASPQHAGVKKGGIFFVLRAGEPIHLLGNNGYRKKLLFRRSRHPP"
     gene            1762..1947
                     /locus_tag="VLB02_03015"
     CDS             1762..1947
                     /locus_tag="VLB02_03015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE57029.1"
                     /translation="MQIYQPVGNPISDIFSYKIEQRIPGWNKVPLPDTTKIASFCFSP
                     CILVAVSKKIEGEVENE"
     gene            complement(1993..2811)
                     /locus_tag="VLB02_03020"
     CDS             complement(1993..2811)
                     /locus_tag="VLB02_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010164978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HSE57030.1"
                     /translation="MKLISWNVNGIRAVHKKGLLVPFIKEYQPDILCLQETKAEQHQS
                     EVDLPEYEEYWNSASKKGYSGTAIFTKEKPLQVLFDFPETITKKYKLADTYGDPNQEG
                     RVVAAEFKKFFVVTVYTPNAKDDLSRIPLRHKGWDPAFLEYMQLLEKNPSAGSGQRKP
                     VIFCGDLNVAHTPDDLARPKENVGNKGFTDEEREGIQKYIDAGFADTFRLFTQGNGHY
                     TWWSHFAGARARNVGWRIDYFFVSEQLVSKVQKAEILPDILGSDHCPILLEIDI"
     gene            complement(2883..3407)
                     /locus_tag="VLB02_03025"
     CDS             complement(2883..3407)
                     /locus_tag="VLB02_03025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE57031.1"
                     /translation="MKNFFVSLSVLFLITLTAGCENKHAAAANIEATAPVEAEKPVPK
                     LSVVTPASVFVGPLDGSEWGGNSEIGKYKEEVINFPLVIEISGKKYPLIVSTSDEWFP
                     EGEVGTETKDPGRTEALKALWDELKKKKAEDFSVTKTDDEKILKVVLNPVYKEGKDGQ
                     VGEIVSPERLIWGQ"
     gene            complement(3565..4083)
                     /locus_tag="VLB02_03030"
     CDS             complement(3565..4083)
                     /locus_tag="VLB02_03030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE57032.1"
                     /translation="MKRKIKVVFGWGFALATLFLIGVSITALENRKPHLLQLEIKQIV
                     VQKKAFSLLEESEYGKHTSLREIREVLLGIKSGNSFFTFPDSTFRDIVLWHVQGTLRE
                     KSVADALKHSTYRSITPNEKLYKARYTGGRFLSWKPYLPSVGEKALYHKPTKLLWFSG
                     STWELLYPEKNF"
     gene            complement(4186..5103)
                     /gene="xerA"
                     /locus_tag="VLB02_03035"
     CDS             complement(4186..5103)
                     /gene="xerA"
                     /locus_tag="VLB02_03035"
                     /inference="COORDINATES: protein motif:HMM:NF040815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008907 - integrase activity [Evidence
                     IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase/integron
                     integrase"
                     /protein_id="HSE57033.1"
                     /translation="MKIEDAKRQFLEYIEIEKGRSQLTVRNYEHYLRQCFEQMQIKKV
                     SDITEECVRQFRLKLNRSSGRKVKGQVSGTMKKVTQNYYMIALRSFLKYWRKRGVETL
                     TPDAIELAKVGARDLDLISLDELSRLLKAPVGDDIKVFRDKAILELFFSTGLRLAELV
                     SLNRDLDLSKDEFSIRGKGEKVRVVFLSDDAKAAIKKYLSARKDMDEALFIDEAKNGS
                     ARKGDRRLTPRSIERLVKHYAIKAGISKKVTPHVLRHSFATDLLSNGADIRSVQMMLG
                     HANIATTQVYTHITDRQLKEIHKKYHSKK"
     gene            5184..5444
                     /gene="rpsO"
                     /locus_tag="VLB02_03040"
     CDS             5184..5444
                     /gene="rpsO"
                     /locus_tag="VLB02_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013485529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HSE57034.1"
                     /translation="MLTSKKKQAVIAKHQLHKKDTGSRDVQIAVLSEAIDKLALHLKK
                     HKKDNHSRRGLIKMVADRRGHLKGLEKEDKPRYEKALKIVKS"
     gene            5579..6088
                     /locus_tag="VLB02_03045"
     CDS             5579..6088
                     /locus_tag="VLB02_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HSE57035.1"
                     /translation="MAIIKQKDQRVGIFIDTQNLYHSAKHLYKRKVNFGNVVKDALAG
                     RKLIRAIAYVITTENEDEKAFLDALSKNGIETKAKDLQIFYDGSKKADWDIGLAIDAV
                     AMAPKLDAIIIISGDGDFVPLCNYLKFHHGVQVEAVAFGQSASGKLREAADDFLDLSV
                     QNKKYLIGY"
     gene            6098..6994
                     /locus_tag="VLB02_03050"
     CDS             6098..6994
                     /locus_tag="VLB02_03050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE57036.1"
                     /translation="MEPEKQQPPVAPNSTPEAAVPPAPAAPPHATLAHSYSEDLAKAM
                     QTTDAAEVQELLSMAREKETVIKQAEEEKKTNWVFVLGTMLCFAVSGAVLWFIWDRFE
                     QAQPVPIERVEQPVIFSQLAPIDTTTTDIRETVQHLRTTATLELNTPTVIPLITAAGE
                     PLSPDEFLKFSEGAIPIGLREQTIALQLGAIATGTTVVPFVLLTGKDQEALTAQLRLA
                     EPELLRGLYRALGIEISELMALFKTPFADDYVSNAPLRVLRKPGAEVPVFLYGFITNQ
                     SVVVTTDTAAFQQIYDAAITGR"
     gene            7040..>7275
                     /locus_tag="VLB02_03055"
     CDS             7040..>7275
                     /locus_tag="VLB02_03055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE57037.1"
                     /translation="MELTHFKEKLEKEKVTIEDELKQIALFNGETSKWEATPDQSMIG
                     EIDENDAADRFEDFESRTSLVTTFQNRLTDVNDAL"
BASE COUNT         2073 a         1646 c         1568 g         1988 t
ORIGIN      
        1 tcgctttcaa gaagatgctt tgcgcctgct ccgagctgtt cgcttcagct gccagcttgg
       61 ctttaccatt tcacatgaaa cattcgaggc gcttgtttcg caggcaggac tcctgcacag
      121 gtttctcacg agcgcattcg ggatgaattt ataaaaataa tagaatcacc aaacccaagc
      181 acgggcattg gcttgcttca aaaaacagga cttttaacct cggttgcgcc tgaattctta
      241 gaaggtgtcg gatgtgaaca gaagggagca catatatacg atgtttttga acacctgctt
      301 cacgcccttc agcatgctgc cgataaggga ttctcgaccg aagtccgact ggcagctcta
      361 ttccacgaca ttggcaagcc tgctacccgc cgatacgatc gcactaagga taagtacact
      421 ttctttggac acgaggtagt aggagctcgg atgaccaaaa agatcctgga acgactcaaa
      481 ttttcacgtg aaacggtttc ccgtgtaaca actctggtgc gaaaccacat gtttttctcg
      541 gataccgaga aaattaccct ttcggctgtt cgccggatta tccggaacgt tggagcagag
      601 cagatttggc ttcttatgga ggttcgtgaa tgcgaccggg tagggatgaa aaagaaagaa
      661 gcgccatacc ggctgcgcaa gtaccacgcc atgatcgaag aagcgctccg tgatcctata
      721 accgtgggac agctcgctat tgacggcacc taccttatgg agacgttaca catgaaaccc
      781 ggaccccgca tgggctggat actccacgcg ctccttgaag aagtactcga aaacccaaaa
      841 ctgaacacca tagagcagct caaagagcga gtcctcgact taaacgaatt agaagataaa
      901 gacttaaaaa ctctcggcga gaagggaaag gagcgaaaag aggaggcaaa tgagggggaa
      961 ctagaggaac tccgcaaaaa gcacggggtg aagtaacact agacagaaaa actctgcgta
     1021 atatacggac caagaaaata ttacctatat ctatatatat tcctatagta aacgttttat
     1081 tttaaagggt acgatcaaca acgccgtcct atttctatag ggcggctttt gttttatata
     1141 gattttatcg atctttgcta tagtatggga aatcgttctt actctatgca aaaattaatt
     1201 accgttcttt ttctctgttt tttacttgaa attacaatca cagagcctac cctgttggct
     1261 gtgagtggga tcatactatt tgaagttcct attaatgccg cctcaccgca acacgcagga
     1321 gtgaagaaag gggggatttt ctttgtcctg cgtgcggggg aacccattca tttacttggt
     1381 aacaatggtt accgaaaaaa gctcctcttc aggagatcac gacatccgcc gtagcggggg
     1441 cttgactcca aagatttttg ctacttagaa aactacgcca cggagcatgc tccgtggttt
     1501 ttttatccat gcgaataggt agtctatttg atttttaacg gtaaccatgt agagtaaaca
     1561 aaaatttata tcatggaata tagaaaaata acctctaaac acaaaggtgt ttctattgta
     1621 ataattgaaa tcggcaaaca tgagaagcct tcaatagctt aaattgtcga tttttgcacc
     1681 acaaacaaaa tagttattct gccaaaaaac tctctggtag atgttactac tccctacctt
     1741 gttaaccaaa aaaaacgggg tatgcaaatt tatcaaccgg taggaaatcc cattagcgat
     1801 attttttctt ataaaattga acagcgaata cctggatgga ataaagttcc acttcctgat
     1861 actaccaaaa tagcttcttt ctgcttctct ccctgcatct tggttgcagt aagtaaaaaa
     1921 atagagggag aagtagagaa tgagtaatgt atacacattt tgccgcagaa agatttctct
     1981 tctgcggttt ttttatatat ctatttcaag caaaatagga caatgatctg aaccaagaat
     2041 atccggtaaa atttcagctt tctgtacctt tgacactaat tgctcgctca caaaaaagta
     2101 atcaatccgc caacccacat ttcgtgcgcg agcaccggca aagtggctcc accatgtgta
     2161 gtgcccgtta ccctgggtaa acaggcgaaa ggtgtcggca aaacctgcat caatatattt
     2221 ttgaatccct tcacgttctt catcagtaaa tcctttgttg cccacgttct cctttggacg
     2281 agcaagatca tcaggagtat gtgccacatt taaatctccg cagaaaataa cgggttttct
     2341 ttgtcctgaa cctgccgaag ggtttttttc aagcagctgc atgtattcta aaaatgctgg
     2401 atcccacccc ttgtgccgca ggggaatgcg cgagaggtca tctttcgcat tcggcgtata
     2461 taccgttacc acaaagaatt ttttaaattc agcggcaaca acacgtccct cttggtttgg
     2521 gtcgccgtac gtatcagcaa gcttatattt tttagtgatt gtctcaggga agtcgaatag
     2581 tacttgtagc ggtttttctt tggtaaaaat cgccgtaccg ctataccctt tttttgatgc
     2641 cgagttccag tactcttcgt attccggcaa atcaacttca gactggtgtt gctcggcttt
     2701 tgtttcctgc aaacacagaa tatcgggctg gtattcctta ataaacggca ctagcaagcc
     2761 cttcttatgc actgcccgaa taccgtttac gttccacgaa atgagtttca tacagagcat
     2821 tgtaacaaaa aagccccaca gtatttgcgg ggcttacaat taactaaact taaaaatgag
     2881 ttttattgtc cccagattaa tctttccggc gaaacaattt ctccaacctg accgtcttta
     2941 ccttctttgt acacaggatt gagaacaacc ttgagtattt tttcgtcgtc cgtcttggtg
     3001 actgaaaaat cctcagcttt tttctttttc agctcatccc atagtgcttt taaagcctcg
     3061 gttcttccag gatctttcgt ttccgtcccc acttctcctt ccggaaacca ttcatctgaa
     3121 gtagaaacaa taagcggata tttttttcct gaaatctcaa ttaccaaggg gaaatttatc
     3181 acctcctcct tatattttcc tatttcacta ttgccgcccc attcagagcc gtccagcggt
     3241 ccaacaaaaa cacttgcggg ggttacgaca ctgagttttg gtaccggttt ttcagcttca
     3301 acaggagctg tagcttctat atttgctgct gcagcatgtt tattttcgca acctgctgtt
     3361 aaggtaatta aaaataaaac ggagagagaa acaaaaaaat ttttcattta gactattttt
     3421 aagttgttta acaaagaaat tactggattc atattagcat aaataagata taaagtcaag
     3481 tattccgtat aggtttaata gcttagaatt acctcgtaat ttataagccc cacagtattt
     3541 gcggggctta tttttaagtg ttttttaaaa atttttctct ggatacagta gctcccaggt
     3601 ggatccggag aaccaaagca gttttgttgg tttatgatag agcgcttttt ctccaacaga
     3661 gggcaggtat ggtttccacg aaagaaacct gccgccggta taccgtgctt tgtacagttt
     3721 ttcgttcgga gtaatactgc gataggtaga atgtttcagt gcatccgcta cacttttttc
     3781 tcgtaaggtt ccttgcacat gccaaagcac aatatctcgg aaagttgagt cggggaaagt
     3841 aaaaaaggaa ttaccactct ttatcccaag caatacttcc cgtatctctc ttaaagaggt
     3901 gtgttttcca tactcactct cttcaagaag agaaaaagct tttttctgca ccacaatctg
     3961 ctttatctct aactgcaata ggtgcggttt tctattttct agagcagtaa tagaaacacc
     4021 gattaaaaag agagtggcta gagcgaagcc ccagccaaaa accactttta tttttctttt
     4081 catatactta gttttaaaag aatctgccac ttataggtag gcaaaatgac ctgttcgtac
     4141 tttgctacgt aaaatatatt ttgtcaagaa aaccaaccct ggtgtttatt tcttactgtg
     4201 atacttcttg tgtatttctt ttaattggcg atcggtaata tgcgtgtaca cctgcgtggt
     4261 agcaatgttg gcatgcccaa gcatcatctg cacactgcgg atatcggcgc cgtttgagag
     4321 gaggtcggta gcaaatgagt ggcggagcac gtgcggcgtt actttttttg aaatgcctgc
     4381 tttgatggcg tagtgtttta ccaggcgctc gatcgagcgg ggggtgagcc tcctatctcc
     4441 ttttcgagct gaaccatttt ttgcctcatc aatgaagagc gcctcgtcca tatccttgcg
     4501 tgcgctcaaa tattttttaa ttgctgcttt agcatcgtct gagagaaaca ccactcgcac
     4561 cttttcaccc ttaccacgga ttgaaaattc atcttttgag agatcgagat cacggttcag
     4621 cgacacgagc tcagctaaac ggagaccagt agagaaaaaa agttctaaaa ttgctttgtc
     4681 acggaaaact tttatatcgt ctcccaccgg cgctttgagg aggcgagaga gctcgtcgag
     4741 cgagatgagg tcgagatcac gcgcgccaac ttttgcaagc tcgattgcat cgggcgtgag
     4801 cgtttcaaca ccgcgcttgc gccagtactt taaaaatgag cggagtgcga tcatgtagta
     4861 gttttgagta actttcttca tcgtgccact cacctgaccc ttcacctttc tgcctgagct
     4921 acggttaagc ttgaggcgaa actggcgcac acactcttca gtgatatctg aaactttttt
     4981 aatctgcatc tgctcaaaac attggcgcag gtagtgctcg tagttgcgca ccgtaagctg
     5041 gctgcgacct ttctcgatct cgatgtactc taaaaactgc cgcttcgcat cctcgatctt
     5101 catacattga gtatactggc tgttcaatta ttgtgcgaca gagggtactt gcttttttta
     5161 taaaactgta ttacaatgct cttatgctaa cgtcaaagaa aaagcaggcg gtcatcgcaa
     5221 agcaccagct ccataagaag gatactggtt cgcgcgatgt gcaaattgca gtcctctcag
     5281 aagctatcga caagctggca ctccacctga agaagcacaa gaaggacaac cactcacggc
     5341 gcggcctcat taaaatggtt gccgaccgac gcggacattt aaaaggtctt gagaaagaag
     5401 acaagccgcg atacgagaaa gccttgaaga ttgtgaagtc ataacaaaaa caagctccct
     5461 tatggggagc ttgttagttt gatgtgcata ttatttttta cgcatctcct tccataatca
     5521 ttttaatttt cttactaaaa cagtagcata tttaaaaata aaacaatata ctttttacat
     5581 ggcaatcata aaacaaaaag atcaacgcgt gggcattttt attgacacgc aaaacctcta
     5641 ccactcggca aagcatttat ataagcgaaa ggtaaacttt ggaaacgtag taaaggatgc
     5701 acttgcggga cgcaagctta ttcgcgctat tgcgtacgtt attacaaccg agaacgaaga
     5761 cgagaaggca ttcctcgatg cgctctcaaa aaatggcatt gaaacaaaag caaaagattt
     5821 gcagattttc tacgacggct cgaagaaagc agactgggac attgggctcg cgattgacgc
     5881 cgtagcaatg gcgccaaagc ttgatgcgat tattattatt tcgggtgacg gcgattttgt
     5941 accgctctgc aactatttaa aattccacca cggcgtacag gttgaagcag ttgcgtttgg
     6001 gcaatcagca tcaggcaaat tgcgtgaagc agctgatgac tttcttgacc tttcagtaca
     6061 gaacaaaaag tatttgattg ggtattaaac aaacggaatg gaaccagaga aacaacagcc
     6121 accagtagca ccgaatagta ctcctgaagc agcggtacct cctgcacctg cagcgccgcc
     6181 ccacgcaaca ctcgcacact catactcgga agatttagca aaggcaatgc aaacaaccga
     6241 tgcagccgaa gtacaagagc tgcttagtat ggcgcgtgag aaagagacgg ttattaaaca
     6301 agccgaagaa gagaaaaaaa caaactgggt attcgtgctc ggcacgatgc tctgttttgc
     6361 ggtgagcggt gcggtactct ggtttatctg ggatcggttt gaacaagcac agccggtacc
     6421 catagagcgt gtagaacaac cggttatttt ttcacaactc gcaccaattg atacaactac
     6481 aacagatatt cgggaaacag tgcaacattt aagaacgact gcgactcttg aactgaacac
     6541 accaactgtt attccgctca tcactgctgc tggcgaaccg ctctcgcctg acgaattttt
     6601 aaaatttagc gaaggagcaa ttcctattgg cttgcgcgag caaaccattg cactccaact
     6661 aggtgcgatt gcaaccggaa ctaccgtcgt accatttgta ctgctcaccg ggaaagatca
     6721 ggaagcgctc acggcacagt tgcggcttgc cgaaccagaa ctattgcgcg gcttataccg
     6781 agcgctcggt atcgagatta gcgagctcat ggcacttttt aaaacaccct ttgccgatga
     6841 ttatgtaagc aacgcgccac tacgggtact gcgaaagccg ggagcagagg ttccggtgtt
     6901 cctgtacggg tttatcacca atcagagcgt tgtagttact accgacacgg ctgcctttca
     6961 gcagatatat gatgcggcta ttacggggcg ctagtaatac acacttgcaa aagcctggcg
     7021 gggtgatacc atttcgggca tggaactaac ccacttcaaa gaaaaattgg aaaaagagaa
     7081 ggtaacgatc gaggacgagc tcaagcagat tgcgctgttt aatggggaaa caagcaagtg
     7141 ggaagcaaca cccgaccagt cgatgattgg cgagattgat gaaaacgacg ctgccgaccg
     7201 gtttgaagat tttgagagcc gcacgagcct ggtaaccacc ttccagaacc gcctcaccga
     7261 tgtcaatgat gcact
//
LOCUS       DATIBQ010000091         4797 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U16294
            k141_715460, whole genome shotgun sequence.
ACCESSION   DATIBQ010000091 DATIBQ010000000
VERSION     DATIBQ010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935789
            Sequence Read Archive: SRR25158331
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 4797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4797)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:58:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 637
            CDSs (total)                      :: 603
            Genes (coding)                    :: 601
            CDSs (with protein)               :: 601
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4797
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_715460"
                     /isolate="SMAG_U16294"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..184
                     /locus_tag="VLB02_03060"
     CDS             <1..184
                     /locus_tag="VLB02_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE57038.1"
                     /translation="EAKERARTVLTKHRKVLDAIAKRLIEVETLEQDAYEAIITAHGI
                     ALKKEKPAKLDLGVEQ"
     gene            191..1774
                     /locus_tag="VLB02_03065"
     CDS             191..1774
                     /locus_tag="VLB02_03065"
                     /inference="COORDINATES: protein motif:HMM:NF021127.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HSE57039.1"
                     /translation="MRRRALWITLGILVLAGVGSGMFSPSSPISVGIHAVKPKAEKKQ
                     QPITVALVGDIMLDRGVARSVAKNFNGDYGALFANVPELAAADIAFGNLEGPVAAGGR
                     NVGSQYSFRMSPEILPALKAAGFDVFSFANNHVGDWSKDAFVETLSYLERAGFQYAGA
                     GDTYADAVAPRIMTVQGKKIGFLAFTDVGPNWLAAKTEVAGVALASDPNLSGIIADAK
                     SHADYLFVSFHFGDEYNPVTARQKKLARQAVDAGADVVVGAHPHVIQATEEYNGKPIF
                     YSLGNFIFDQYFSPETMRGGLVLVTITPEGKISFEEKTVDLNTFYQPQAPRPRTAGDA
                     VLVAKAVPFSCPKPKTLAADYFLLPLPSETVDLKNYIPNNLVQISRTVTGGADICLTK
                     ETYTAYQSMLTAMKKEKVEAHVFSGFRDAAMQRQNLQERGDSIAAAVGQSEHHLGTTI
                     DFTSGTVPVKFIESPEYTWLQEHAWEYGFVQSYRPGQGDANKGGAGGESWHWRYVGKT
                     HAKKIHREDVPLKEYLENL"
     gene            complement(1771..2247)
                     /locus_tag="VLB02_03070"
     CDS             complement(1771..2247)
                     /locus_tag="VLB02_03070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE57040.1"
                     /translation="MTNEFVAKKIGELVAFTMVSGEILDRGGAPLLEKLGQETVLDIT
                     EKNQLHGETLRRFAEEAGVLDTVTAKTEKTAEKLRKLHEVYIGDAWQNPVEVCEWLGF
                     FEGAAIVHWELLRGAAATINNEALLVLTEEAVTYHYELLEKFGSELEEVGQAKAAD"
     gene            2333..2896
                     /locus_tag="VLB02_03075"
     CDS             2333..2896
                     /locus_tag="VLB02_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUMOD4 domain-containing protein"
                     /protein_id="HSE57041.1"
                     /translation="MVQKEIWSLLKFPGQKNARRAYAVSSLGRIRSFRQRETPENGKL
                     LQGSITSGYRILNFCHLEICCVLYIHREMAKLFLKKPSSRHRFVIHLNHQKLDNRVKN
                     LKWVTADEMHAHAQRSPAKIAYKKAQANRTKGLKLTAAQVKNIKKVLANKNRSITIKE
                     LAAKYDVSTMTIYRIKGGSNWSHVQLK"
     gene            2980..3052
                     /locus_tag="VLB02_03080"
     tRNA            2980..3052
                     /locus_tag="VLB02_03080"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3013..3015,aa:Ile,seq:tat)
     gene            3520..3915
                     /locus_tag="VLB02_03085"
     CDS             3520..3915
                     /locus_tag="VLB02_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020525482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4383 domain-containing protein"
                     /protein_id="HSE57042.1"
                     /translation="MNTQKLAKLFGWVFLIIGVLGFVPGITGNGHLLGIFEVDSLHNI
                     IHLLSGVLALWLGKSESGAKTYFKVFGVVYALVTVLGFLSGTVLGLISVNLADNVLHL
                     VIAAIALWAGFSGSKPAMQMPPQQGGMKM"
     gene            complement(3976..>4797)
                     /locus_tag="VLB02_03090"
     CDS             complement(3976..>4797)
                     /locus_tag="VLB02_03090"
                     /inference="COORDINATES: protein motif:HMM:NF012493.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HSE57043.1"
                     /translation="SMTATPIPRTLALTMYGDLDLSLLDEMPVGRKPVLTTVVSPANR
                     AGVYEAIRAQLREGRQLYIICPRIAEADPSILGSLQAKSVTEEAKRIQKAVFPEFSIG
                     TLHSKMTKEKKEAAMSDFVNHKTDILVATSVIEVGINVPNATIIIIEGAERFGLAQLH
                     QLRGRVARSSIQAHCYLFADAKTEKTAERLRLFTKAKNGFELAEYDLALRGTGELSGL
                     KQWGVSDLAMDSLKNIKLVEAAREEAKKLLAQDTTLSKYPLLQVAIKKTENMHFE"
BASE COUNT         1275 a         1044 c         1151 g         1327 t
ORIGIN      
        1 agaagcgaaa gagcgtgcac gcaccgtgct caccaagcat cgcaaagtac ttgatgcaat
       61 tgccaagcgg ctgatagaag tagaaacact cgagcaggat gcgtacgagg ctattattac
      121 tgctcatggc attgcgctca aaaaagaaaa acctgcaaag ctcgatcttg gagtagagca
      181 gtaaaaaatt atgcggcgcc gtgcactgtg gatcacactc ggaatactcg ttctggctgg
      241 tgttgggtcg ggaatgtttt ctccatcctc gccaatttca gttggtattc atgcggtaaa
      301 gccgaaagcg gaaaaaaagc agcagccgat tacggttgca ctggtaggcg atatcatgct
      361 cgaccgcggg gtcgcgcgtt cagtggcaaa gaattttaac ggagactacg gtgcgctttt
      421 tgcaaatgtt cccgagcttg ccgctgcaga tattgcattc ggaaatttag aaggtcctgt
      481 tgcggcgggc gggcgcaatg tggggagtca gtactcattt cggatgagtc ccgagatact
      541 gccggcgctc aaggcggcag ggtttgacgt gttctcgttt gcaaacaacc acgtcggtga
      601 ttggtcgaag gatgcatttg tagaaacact atcgtattta gaacgggcag gtttccagta
      661 tgcaggtgca ggggatacct atgcagatgc ggtagcgcca aggattatga cggtgcaggg
      721 gaagaagata ggatttctgg cgttcactga tgttggtccg aattggcttg ctgcaaaaac
      781 ggaagttgcc ggcgtggcgc tcgccagcga tccgaatctc tcaggaatta tcgctgatgc
      841 aaaatcccat gctgattatt tatttgtatc gttccatttt ggcgatgagt acaatccggt
      901 aactgctcgg caaaaaaagc ttgcccgcca ggcggtagat gctggtgccg atgtggtagt
      961 aggtgcacac ccgcatgtga ttcaagccac cgaagaatac aacggcaagc caatttttta
     1021 cagtcttgga aattttattt ttgaccagta tttctctccc gagacgatgc gcggcgggct
     1081 cgtactcgtt actattaccc ctgagggcaa gatttctttt gaagaaaaga ccgtagacct
     1141 gaacacattt tatcagccgc aggcaccgcg cccgcgcact gcgggtgatg cagtacttgt
     1201 tgcaaaggca gtacccttta gctgtccaaa accaaaaacg ttggcagccg attatttttt
     1261 actcccgctt ccgagtgaaa ctgttgattt aaaaaattac attccaaata acctagtaca
     1321 gatttctcgt acggtaactg gcggggcaga catttgcctt accaaagaaa catacactgc
     1381 gtaccagagc atgctcaccg cgatgaaaaa agaaaaagta gaagcgcatg tgttctcggg
     1441 ctttcgtgat gcagcgatgc agcggcagaa cctgcaggag cgaggggata gtattgctgc
     1501 tgcggttggg caatcggagc accacctggg aacgacgatt gattttactt cagggacggt
     1561 gccggtaaaa tttattgaaa gccccgagta tacttggctc caggagcatg cctgggaata
     1621 tggctttgtg cagagttacc gcccggggca gggtgatgct aataaaggag gagccggcgg
     1681 agaatcgtgg cactggcggt atgtaggaaa aacccatgca aaaaagatac atcgagaaga
     1741 tgtaccgctc aaagagtatc ttgaaaatct ttaatcagct gcttttgcct gccctacttc
     1801 ttcaagttca ctgccgaatt tttctaacag ctcgtagtgg taggtaactg cttcttcggt
     1861 aagcacaagc agtgcttcat tatttattgt tgctgctgca ccgcggagca gttcccagtg
     1921 tacaattgct gccccttcaa aaaaaccgag ccactcgcac acctcaaccg gattttgcca
     1981 ggcatcgcca atgtacactt cgtgtagttt ccgcagtttt tctgcggtct tttctgtttt
     2041 tgcagttacc gtgtcgagca cgccagcttc ttctgcaaaa cgccgcagtg tttcgccatg
     2101 gagctggttt ttttcagtaa tatcgagcac tgtttcttgt ccgagcttct cgaggagcgg
     2161 tgcaccgccc cgatctagaa tctcaccgct taccatagta aatgctacca gttccccaat
     2221 tttttttgct acaaattcgt ttgtcatatg tagctattgt agcaggaacg tacacttgct
     2281 gatgctcttg acaatttttt aaaattgagt atttttaaga aaaattttat gtatggtaca
     2341 aaaagaaata tggtcattat taaaatttcc tggacagaaa aatgcgcggc gagcgtacgc
     2401 agtttcttca cttggtcgta ttcgtagttt tcgccaaaga gaaacaccag aaaacggaaa
     2461 attgttacaa gggtcaatta ctagcggcta ccgaatacta aacttctgcc atttagaaat
     2521 ctgttgcgtt ttgtatattc accgcgaaat ggcgaaattg tttttaaaga aaccatcatc
     2581 tcgtcacagg tttgttattc acttgaacca tcaaaaactt gataatcgag ttaaaaattt
     2641 gaaatgggta acggctgatg aaatgcacgc gcatgcgcag cggagtcctg ccaagattgc
     2701 gtacaaaaaa gcacaggcga atcgtaccaa gggtttgaag ctcactgctg ctcaggtaaa
     2761 gaatattaaa aaagttcttg ctaataagaa ccgaagcatt accattaaag aattagcggc
     2821 aaagtacgat gtcagtacaa tgactatcta ccgcatcaaa ggaggctcta actggagtca
     2881 tgtacaatta aaataaaatt cacatcctcg tcgcatcagc gacggggttt tctttttcca
     2941 aaaatcacta tagaattgcc atggtttaaa gctaccacag ggagtatagc tcagttggta
     3001 gagcgccgag cttatacctc ggtggtcgat ggttcgagtc catctactcc cacccgtttt
     3061 gacaagcaga agaaaataaa gcaaactaaa tttgaaattg attattttat ttccacctac
     3121 atttaaatag gacttatgat tataagaata aaccgaacag aagaaatcag aaaccgctta
     3181 cagaaagagg gcaagagcag ataccttagt tcaccttcag atattcaggg aatgattcgt
     3241 atgaatgaag agttacgagg attgcgccgt gatttcatta ctaaaagtaa gcgttctgaa
     3301 atatcggcag ctcaaacaat tcttaatgca tgatacctgt catcaaatat ttaaagagct
     3361 cattgagctc ttttttatgt attaatttat taaacgtgta cagcttttga attgttgtgg
     3421 attactgttc tattattttt tacaaacgcg atatactata tacgattact cctgcaatgg
     3481 tcatgcatgg taatcgagtg gattcacttt cttatattta tgaatacaca aaaactagca
     3541 aaactgttcg ggtgggtgtt cctcatcatt ggagtgcttg gatttgttcc aggcattacc
     3601 ggcaatgggc acctcctcgg catattcgaa gttgattcgc tccacaatat cattcacctc
     3661 ctttcaggtg tattggcatt atggctcggc aagagcgaat cgggtgcaaa aacctacttt
     3721 aaggtttttg gtgttgtcta tgcactagtt accgtactcg gcttcctgag tggaaccgta
     3781 ctcggactca ttagtgtgaa cttggccgac aacgtactcc acctcgttat tgcagcgatc
     3841 gcactctggg caggtttttc aggaagtaaa ccagcaatgc aaatgcctcc gcaacagggc
     3901 gggatgaaga tgtagggcag gtatacgttc tataaaaact cccgctttgg cgggtgtttt
     3961 tgttttttgc agtgattatt caaaatgcat gttttctgtt tttttgatgg caacttgcaa
     4021 gagtgggtat ttagagaggg tagtatcttg tgcgagcagc ttttttgcct cttcgcgagc
     4081 tgcttcaacc agttttatat ttttgagcga atccattgcc aagtctgaca cgccccattg
     4141 tttgagtccc gagagttcac cagtaccgcg cagtgccaag tcgtactcgg caagctcgaa
     4201 cccatttttt gctttggtga ataatcgcag ccgttcggca gttttttcag tttttgcatc
     4261 agcaaataaa tagcagtgcg cctggatgct gctgcgggca acgcgtccgc gcagctggtg
     4321 gagctgggcg agcccaaatc gttctgcgcc ttcaatgata attatggtcg catttggtac
     4381 attgatgccg acttcaataa ctgaagttgc aaccaagata tcggttttat ggtttacaaa
     4441 atcactcatt gctgcctcct ttttttcttt agtcatcttg ctatgcagtg tgccgattga
     4501 aaattctgga aaaacagctt tttgaattcg cttcgcttct tcagtaaccg actttgcctg
     4561 caagctgccg agtatactcg gatctgcttc ggcaatgcgc gggcagataa tgtagagctg
     4621 tcgcccttcg cgcagctgtg ctctgattgc ttcatacaca cctgcgcggt tagcaggaga
     4681 aacaacagtg gtgagtactg gttttcgtcc caccggcatt tcatcaagaa gcgagaggtc
     4741 aaggtcgccg tacatagtaa gcgcaagagt gcgcgggatt ggagtggcag tcattga
//
LOCUS       DATIBQ010000092         2753 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U16294
            k141_2056352, whole genome shotgun sequence.
ACCESSION   DATIBQ010000092 DATIBQ010000000
VERSION     DATIBQ010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935789
            Sequence Read Archive: SRR25158331
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:58:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 637
            CDSs (total)                      :: 603
            Genes (coding)                    :: 601
            CDSs (with protein)               :: 601
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2753
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2056352"
                     /isolate="SMAG_U16294"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            3..152
                     /locus_tag="VLB02_03095"
     CDS             3..152
                     /locus_tag="VLB02_03095"
                     /inference="COORDINATES: protein motif:HMM:NF013440.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HSE57044.1"
                     /translation="MGSEETDMLSGKISYKSPLGTALVNKKVGDKVLVHSPKGEIEYK
                     ILEIK"
     gene            267..701
                     /gene="mraZ"
                     /locus_tag="VLB02_03100"
     CDS             267..701
                     /gene="mraZ"
                     /locus_tag="VLB02_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HSE57045.1"
                     /translation="MIIGQYLHTLDDKNRLSFPAKFRKEVGKTVIITPGLDQCLFVFT
                     TKEWAAISARLSESSLLQADNRSFHRYLFGGAVEVSFDTLGRVLIPDFLKEKAGLQGK
                     VAIIGVHNRIELWDTGTWDKYKRDIEGRADALAEKLGSVGAL"
     gene            704..1579
                     /gene="rsmH"
                     /locus_tag="VLB02_03105"
     CDS             704..1579
                     /gene="rsmH"
                     /locus_tag="VLB02_03105"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:NF013919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HSE57046.1"
                     /translation="MHIPVLLHEAIDTLSVEPGNVVVDGTYGGGGHTNELLKQFGETV
                     RIICVDLDEQAEERFKAAQYPSTVQFVHQNFKNASEILAAAGVEHADRVLLDLGTSTF
                     QLLEDTRGFSFRSDTPLRMTFSAAGAHTNTSAYDVVNGWGEETLETIFKGFGEERRAR
                     TIARAIVTAREKTPITSSGQLAALVEAAIGRRGKTHPATRVFQAIRIAVNEELGVLEA
                     AITNWFSALPAGGRLAIITFHSLEDRIVKRAFASYHQKQFGRVITKKPLVPKRAELIS
                     NPRSRSAKLRSIEKL"
     gene            1588..1914
                     /locus_tag="VLB02_03110"
     CDS             1588..1914
                     /locus_tag="VLB02_03110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE57047.1"
                     /translation="MATTIKQYEFFSNGGLLAHSTKALWALIGLTFVAYVYCVGSITF
                     SVVERQRLDHEVKELLSDISAEELSYLALDRSLTRQEAYALGLAESHEVSFSTRGTGF
                     AFNADR"
     gene            1901..>2753
                     /locus_tag="VLB02_03115"
     CDS             1901..>2753
                     /locus_tag="VLB02_03115"
                     /inference="COORDINATES: protein motif:HMM:NF015662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE57048.1"
                     /translation="MQTANIHRTRIQIVMVLVILLGALFSVRLFMLQVVQAGKFTERA
                     ERQYVTPSSSVFDRGTIYFTKRDGTTVAAATVASGFKLAIAPAQITDSEAVYSALRQY
                     IELDRQDFFSRAAKKNDPYEEIANRISKANADAITALELPGVSIFKEKWRFYPGGSLA
                     AKTLGFVSYKQDTLLGRYGLERSYNEVLTRNPDSFYVNFFAEIFANLQSTIFKNKQTI
                     GDVVTTIEPSVQSMLEAAVERVESQWQSDGVGGIVMDPHTGDILALAHVPSFDLNEYG
                     KVSDVSRY"
BASE COUNT          723 a          660 c          697 g          673 t
ORIGIN      
        1 tggtggggag cgaagaaacc gacatgctct cgggtaaaat ttcatacaaa tcaccgctcg
       61 gcacggcgct tgtcaataaa aaagtaggcg acaaggtact cgtccacagc cccaaaggcg
      121 aaattgagta taaaatttta gaaattaaat aattctctaa aaatcccctc cacttggagg
      181 ggatttttag ttatccccat attgcagctg gtagtgggtg gtatacaatg taatcaagtg
      241 gaaagaagtg gaaaaaataa atttgcatga taatcggaca atacctacac acacttgatg
      301 ataaaaatcg cctttcattt cctgctaaat tccgcaagga ggtggggaag acggtgatta
      361 ttactcccgg actcgatcag tgtttgttcg tatttaccac caaggaatgg gcagccattt
      421 ccgcccggct ctcagaatcg agcctgctac aggcggacaa ccgctcgttc caccggtacc
      481 tgttcggcgg cgcagtagaa gttagttttg acactcttgg ccgagtgctg attccagact
      541 ttttgaaaga aaaagccggc ttacagggaa aggtcgccat tattggtgtc cacaaccgca
      601 ttgaactgtg ggatacaggc acctgggaca agtacaagcg agacatcgag ggcagggctg
      661 atgcactcgc cgaaaaactt ggttcagttg gcgcattgta atcatgcata tacccgttct
      721 tttacatgag gcaatagata cactttcagt cgagcccggc aacgtagttg ttgacggtac
      781 ctacggcggc ggcggacata cgaatgaact cttgaagcag tttggcgaaa cagtgcgcat
      841 tatctgcgtt gatctcgacg agcaggcgga ggagcgattt aaagcagcgc agtacccgag
      901 cacggtacag tttgtgcacc agaattttaa aaacgcgagc gagatacttg cagctgccgg
      961 tgtggaacat gccgaccgtg tgctcctgga tttagggacg agtacttttc aacttttaga
     1021 agacacgcgc ggattctcat ttcgttcaga tactccgctg cgaatgacct tctcggctgc
     1081 cggtgcgcac accaatacca gtgcgtacga tgtggtgaat ggctggggcg aagaaacact
     1141 tgaaaccatt tttaaaggat tcggtgaaga gcgccgggcg cgcaccattg ctcgtgcaat
     1201 tgttactgct cgagaaaaaa cacccatcac ttcaagcggg cagcttgcag cacttgttga
     1261 ggctgccatc ggcagacgag ggaagaccca tcccgcaacg cgtgtgtttc aagcaatccg
     1321 catcgcagtg aatgaagaac tcggcgtact ggaagcagca atcacaaact ggttttcagc
     1381 actgccggcg ggtggcagac tcgcgattat cacctttcac agtttagaag accgtattgt
     1441 caagcgtgct tttgcctcat atcatcaaaa acagtttgga cgcgttatca ccaaaaaacc
     1501 gctcgtgcca aaacgagcag aacttattag taacccccgc tcacggagcg cgaagctccg
     1561 cagtatagaa aaactatgat ccgcagtatg gcaaccacaa ttaaacagta cgagttcttt
     1621 tcaaacggag gactgcttgc ccattcgacc aaggcgctct gggcgctcat cgggcttacc
     1681 tttgttgcct acgtgtactg tgtgggttcg atcacgtttt cagtagttga gcgacagcgc
     1741 ctcgaccacg aggtaaaaga gcttctctct gacatcagtg cagaggagct ttcctacctc
     1801 gcgcttgatc ggagccttac ccggcaagag gcgtatgcgc tcggacttgc cgagagccat
     1861 gaggtaagct tctctactcg aggaactgga tttgcattca atgcagaccg ctaacattca
     1921 ccgtactcga atacagattg tgatggtact cgtcatcctc ttgggtgcac ttttttctgt
     1981 ccgcctgttt atgctccagg tggtgcaagc gggaaagttt accgagcgcg ccgaacggca
     2041 gtacgttacc ccttcaagca gtgtgtttga ccgcggtact atttatttta caaagcgaga
     2101 cggaactacc gttgctgcag caacggtagc ctcggggttt aagcttgcca ttgctccagc
     2161 acaaattacc gatagtgaag cagtgtacag cgcgcttcgt cagtacattg aacttgaccg
     2221 gcaggatttt ttttcacgag cagcgaaaaa aaatgatcca tacgaggaaa tcgcaaatcg
     2281 tatttctaaa gcgaatgccg atgcgattac cgcgcttgaa cttccgggcg tttctatttt
     2341 taaagagaag tggcgctttt accccggtgg ttcacttgct gcaaaaacgc tcggttttgt
     2401 ttcgtacaag caagatactc ttttagggcg ctacgggctc gagcgatcct ataacgaagt
     2461 actcacccga aaccccgaca gtttttatgt aaactttttt gcagaaatat ttgcaaattt
     2521 gcagtcaact atttttaaaa ataagcaaac cattggtgat gtagtaacca ctattgaacc
     2581 atcagtccaa agcatgcttg aagccgcagt tgagcgcgta gagagccagt ggcagagtga
     2641 tggggtgggc ggcattgtga tggatccgca cacgggcgac atactggcgc ttgcacacgt
     2701 gccctcgttt gatcttaacg agtacggaaa agtgagcgat gtttctcgct atg
//
LOCUS       DATIBQ010000093         7600 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U16294
            k141_2269996, whole genome shotgun sequence.
ACCESSION   DATIBQ010000093 DATIBQ010000000
VERSION     DATIBQ010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935789
            Sequence Read Archive: SRR25158331
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 7600)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7600)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:58:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 637
            CDSs (total)                      :: 603
            Genes (coding)                    :: 601
            CDSs (with protein)               :: 601
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7600
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2269996"
                     /isolate="SMAG_U16294"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(28..378)
                     /gene="rplU"
                     /locus_tag="VLB02_03120"
     CDS             complement(28..378)
                     /gene="rplU"
                     /locus_tag="VLB02_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009752820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HSE57049.1"
                     /translation="MATKQAKKAENTEFAVIKTGGKQYQVSVGDVITIEKLKGEPKKG
                     DSVVFDEVLMVESGAATTIGTPFIAGATVTGTVEMVGKKPTVTVIKYKAKSRYFKKNG
                     HRQPYCKVKVTAIK"
     gene            463..1368
                     /locus_tag="VLB02_03125"
     CDS             463..1368
                     /locus_tag="VLB02_03125"
                     /inference="COORDINATES: protein motif:HMM:NF013694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HSE57050.1"
                     /translation="MISRYQYKELVWVDLESPTQEEVRHIMDEFDLPHLVGEELVSNT
                     LRSKVDLYENLLYVILHFPHVTHGVSVEQEVDFVIGEHFLITARYETIDPLHDFSKIF
                     ETHSVMDKSKLPGHAGIIFMQMLKELYRHSLHELESITKTLQSIEREIYAGHEARMVR
                     TIAATGKKLLDFKQAIRFHADILHSYEIASKQFFGTEYGYYASAITAEFNKVHSLLEG
                     HRDMLSELQRTNDSLLTSKSNDIMKTFTVMTFVMLPLTLITGIFGMNADFVFIRGLQD
                     FYFVIAAMAITAIIMFIFFRIRKWL"
     gene            1359..2735
                     /gene="cysS"
                     /locus_tag="VLB02_03130"
     CDS             1359..2735
                     /gene="cysS"
                     /locus_tag="VLB02_03130"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HSE57051.1"
                     /translation="MALILYNTKTRKLEAFTPITPGKVGMYTCGPTVYGPAHIGNLRA
                     YVFADLLKRTLIANGYAVEHVINITDIGHLVGDGDEGEDRMTKALKRENLPLTMEAMY
                     QLGTRYLEKFKDDIEKLSIITPTVLPRAADHIQEDIALVAILIEKGFTYATNDGIYFD
                     TTKMSTYGEFARLPKEEATEEHQRIVNSKKKNSRDFALWKFDAELGYEAPFGHGFPGW
                     HIECSAMAMKYLGETFDIHTGGIDHIPVHHQNEIAQSESATGKEYVRYWLHNNFLNVG
                     GEKISKSLKNDIILDEIIAQGIAPAALRLFFLTAQYQSFQNFTWDALNAAQKAWNKIS
                     AFMMQENPNGSVNDTYYALVLEKMNNNLNTPEALAVLWDLFKADIPAPEKRATLVAVD
                     KLLGLGLETIPQVTVPPEVAEKLRVRNSARENKNYTEADALRAEIRAEGFDIIDTESG
                     SFAIPAKK"
     gene            complement(2800..3246)
                     /locus_tag="VLB02_03135"
     CDS             complement(2800..3246)
                     /locus_tag="VLB02_03135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE57052.1"
                     /translation="MAIFCCSILLHANPIEGAEKAIESFKKTFSTAENVSWYKTGEHE
                     NCYYHVSFLLGVFQIKATYNQQGDMVSGIRYYSPLYLPPNIATTLKKEYGGKTLFGVT
                     ETTNAEGAVTYFVKMYDDKKWYTVKIFPSGEFIEYEKYKRADIGIN"
     gene            complement(3368..4213)
                     /locus_tag="VLB02_03140"
     CDS             complement(3368..4213)
                     /locus_tag="VLB02_03140"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HSE57053.1"
                     /translation="MPDPADTNRVTVNELQKATEELYKQNFELATLYKKVEQLNGDLE
                     VANSKLKDLDKLKTEFLSLASHQLRAPLTAIKGYASMLEEEAYGKLTTEQAGAIKRIY
                     VSAQGLSSLVEDLLNISKIEQGGLKFEFEETDLGKLLTDLYNEIRITAEDKGLEFTID
                     IPSNQSFMAHVDRLKLKQAFLNLADNSIKYTPHGFVKLSLRREGNTIVYQVADNGIGI
                     SPETKAKLFDKFSRGEGGRLNTGGSGLGLYLAKQIILAHKGDIAIESDGVGKGSKFTV
                     TIPSM"
     gene            complement(4220..4498)
                     /locus_tag="VLB02_03145"
     CDS             complement(4220..4498)
                     /locus_tag="VLB02_03145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE57054.1"
                     /translation="MTIFDQISIRIIKEQELIIGPVAWFEAQKVPGVIANSKEGKISL
                     RGDGKETIDKLVAQYERLFGRASHEVCKEATKDLIATLPLDQIPSSLR"
     gene            complement(4503..5336)
                     /locus_tag="VLB02_03150"
     CDS             complement(4503..5336)
                     /locus_tag="VLB02_03150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE57055.1"
                     /translation="MKINTYQKFFLTFLALILVFWAVLFLSDIKEGFYNYLYSFLFGL
                     IPLIGGIIGMRGAQSWGGLKSAVGKALFFISFGIFLWGCGEAIWSYYNFFEGVPAPYP
                     SLADLGFAPSIFFYGLGSIYLAKATGAKFGLRSTGAKIFAYLAPIAIFVFSYYVLVTV
                     ARGGVLVPEGESLLKTILDIAYPVGDFIGLAVSVVISGLSFKYLGGRYILDIASILLG
                     LAVMFIADSIFSYTTTAGTYYNANFGDLILTLGVFFLAFGVVGFCNHIKSEKVSVHTV
                     Q"
     gene            complement(5379..6527)
                     /locus_tag="VLB02_03155"
     CDS             complement(5379..6527)
                     /locus_tag="VLB02_03155"
                     /inference="COORDINATES: protein motif:HMM:NF014803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III PLP-dependent enzyme"
                     /protein_id="HSE57056.1"
                     /translation="MPNTESPQNPFTREILEIYNQETPFFLFSKERLLAKYKEFEESF
                     PGAAIHYAMKANAEPEILRELFDAGAHFEVASVYELETLKEVGVPGERIVYGTSVKPP
                     FHIREFAAYGVDRFAFDSFLELEKIAALVPGARVYARISVDDTGSVFKFSEKFGTAKE
                     NALPMLERARELGLVPYGISFHVGSQAGNERAWAEAIALLRPTLEKLLEADIKLEVID
                     IGGGFPCNKYTSTENDFTLQEIAAHTLQEYKNLPYQPKLLLEPGRAIVAETGILVTNV
                     IARIERREHTWLFLDAGVYSGLFESMVYQGSTRYRTTLLRPSFDVGEAPFALAGPTGD
                     SPDVITREVFLPADVAIGDKLIIHDVGAYSLVATCPFNGFPKPKVYFI"
     gene            complement(6555..7451)
                     /locus_tag="VLB02_03160"
     CDS             complement(6555..7451)
                     /locus_tag="VLB02_03160"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HSE57057.1"
                     /translation="MHQELIIALLAGLGGMLGWGLADFFAKKTIDEVGDIQSLAWAHV
                     FGTGFFFIIAVYKFLVLGRPIVFLNPGSSLLYLIFFGVLQAIIYILVYRGFGKGQVSI
                     LNPVFASFSGIAALISIVGFGEVVSSNLVIALVIIFGGVLLINTDFSKLHLGKFNFVQ
                     VPGFKEIAAATALAAVWTVGWDKVVGGTDWVIYSFLMYAFMTVAILLFAKVKKVDLCV
                     IKKPIWKFLLLIGFCETVAYLAISLGYSATTKTSVIALLSGAFSLPTIFLARSFLKER
                     VTVTQTIGGFVIVIGIILLSVL"
     gene            complement(7455..>7600)
                     /locus_tag="VLB02_03165"
     CDS             complement(7455..>7600)
                     /locus_tag="VLB02_03165"
                     /inference="COORDINATES: protein motif:HMM:NF012379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HSE57058.1"
                     /translation="FDTEFVEALEYGMPPTCGFGMSERVFAFLANKTVREAQLFPLLK
                     PKG"
BASE COUNT         2336 a         1713 c         1432 g         2119 t
ORIGIN      
        1 ataatttctc ggttttttca aataaattta cttaatcgcg gtaactttta ctttacagta
       61 cggctggcgg tgaccgtttt tcttgaagta tcggctcttt gccttgtact taataacggt
      121 tacagttggc ttcttaccca ccatttctac tgttccggtt acggtggcac cggcaataaa
      181 gggtgtgccg atagtggttg cagcaccgct ctctaccatt aacacttcat caaaaacaac
      241 actatcgcct ttctttggct ctcctttcag tttttcaata gtaataacat cacctactga
      301 aacctggtac tgcttgccgc ctgttttaat aacagcaaat tcggtattct ctgctttttt
      361 tgcttgtttc gtagccataa atgagtctcg gtattataca gagcggcaca aaaaatgcaa
      421 gcaggaagcc tctttttgcc aagagtgcta tactttaagg cgatgatatc tcgctaccag
      481 tacaaagagc ttgtgtgggt tgaccttgag tctcctaccc aggaagaagt acgccatatt
      541 atggatgagt ttgacctccc gcacctggta ggagaagagc ttgtttcaaa tactctgcga
      601 tcaaaagtag atttatacga aaacttgctc tacgtcattc tccactttcc gcatgttacg
      661 catggtgttt cggttgagca agaagttgat tttgtcattg gcgagcactt tttgattact
      721 gcccgctacg aaaccatcga cccactacac gatttttcaa aaatatttga aacgcattca
      781 gtcatggaca aaagcaagct gccggggcac gcaggtatta tttttatgca gatgctcaaa
      841 gagctctatc ggcactcgct ccacgaactt gaaagtatta caaaaacact tcaatcaatt
      901 gaacgtgaaa tatacgccgg gcatgaagcg cggatggtgc gcaccattgc cgcaacgggt
      961 aaaaaactgc tcgacttcaa acaggcaatc cgctttcatg ctgacatatt gcactcgtat
     1021 gagatagcca gtaagcaatt ttttggcact gagtacggct actatgcttc ggcaattacc
     1081 gccgagttta acaaggtgca cagcctactc gaggggcatc gcgacatgct ttctgaactg
     1141 cagcggacaa atgattcact cctcactagt aaatcaaacg atattatgaa aacgtttacg
     1201 gtcatgacat tcgtgatgct gccgctcacc cttataacgg ggatattcgg catgaatgcc
     1261 gacttcgtct ttattcgcgg tctgcaagat ttttactttg ttatcgcagc aatggccatt
     1321 accgctatta tcatgttcat ctttttccgc atcagaaaat ggctctaatt ctctacaaca
     1381 ctaaaaccag aaaactcgaa gcgtttaccc caattacccc cggtaaggtg gggatgtaca
     1441 cctgtggtcc aaccgtgtat ggaccagcac atattggaaa tttgcgcgcc tacgtttttg
     1501 ctgacctatt aaagcgaaca ctcatcgcaa acgggtacgc cgtagagcat gtaattaaca
     1561 tcaccgacat cggacactta gttggtgatg gcgatgaagg cgaggataga atgaccaagg
     1621 cgcttaagcg agaaaactta ccgctcacta tggaggcaat gtaccagctc ggcactcgat
     1681 acctggaaaa atttaaagat gatatagaaa aactctcaat aattacccca acggtactcc
     1741 cccgcgctgc cgaccatatt caagaagata ttgctcttgt ggcaatactc attgaaaaag
     1801 gctttaccta cgctactaat gacggcatct attttgacac gactaagatg tcaacctatg
     1861 gcgagtttgc ccggcttcct aaagaagaag caactgaaga gcatcaacgg atcgtgaact
     1921 ccaaaaaaaa gaattcgcgt gattttgctc tgtggaagtt tgatgccgag cttggctacg
     1981 aagcaccttt tgggcacggc ttccccggct ggcacattga atgctcggcg atggcgatga
     2041 agtatttagg agaaaccttc gacatccaca cgggcggtat tgaccacatc cctgtgcatc
     2101 accaaaacga gattgcccag tcggaatcag caaccggaaa agagtatgtt cggtactggc
     2161 tgcacaataa ttttttaaac gtcggcggtg aaaaaatttc aaaatcgcta aaaaacgata
     2221 ttattcttga tgaaattatt gctcaaggaa ttgctcctgc agcacttcga ttattctttc
     2281 ttacggcgca gtatcaatcg ttccaaaatt ttacttggga tgctctgaat gctgcgcaaa
     2341 aagcgtggaa taaaattagt gcctttatga tgcaagaaaa tccgaatggc agcgtaaatg
     2401 acacctacta cgccctggta cttgaaaaaa tgaataataa tttaaatacc cctgaagcac
     2461 ttgcggtact gtgggatctc ttcaaagccg acattcctgc tcctgaaaaa cgagcaacac
     2521 tggttgcagt agacaaactc ctaggactcg ggcttgagac tattccgcaa gtaaccgttc
     2581 cgcctgaggt agctgaaaaa ttgagagttc gtaatagcgc ccgcgaaaat aaaaattaca
     2641 ctgaagccga cgcactccgt gctgaaattc gtgccgaagg cttcgatatt atcgataccg
     2701 aatcaggcag tttcgcaatt ccagcaaaaa agtaatgatg tattaaaaaa ccaccggaac
     2761 attccggtgg ttagagcttt tgagaaagat ttttgttatt taattgatac caatatcagc
     2821 tcgtttatat ttttcatatt ctataaactc tcctgagggg aaaattttta ccgtatacca
     2881 ttttttgtcg tcatacattt taacaaaata ggtaacagcc ccctcagcgt ttgtcgtttc
     2941 tgttacccca aacaatgttt taccgccata ctcttttttg agagtagtag caatatttgg
     3001 tggaagatac aagggagaat agtaacggat tccagaaacc atatctccct gctgattgta
     3061 agtggctttt atctggaaaa cacctaacaa gaacgaaaca tggtagtagc aattttcatg
     3121 ttctcctgtt ttataccagg aaacattttc tgcagtagaa aaagtttttt taaaactttc
     3181 aatagctttt tcagccccct caatagggtt ggcatgcagc agaatagagc aacaaaaaat
     3241 tgccaaaatg gcaagcagta gttttttcat aacttaaaac gtttttgttt ttaaaaaact
     3301 cccacaatag cgtgggttta tccaaaaata aaatagcatt ttttagggaa tacgtcaaaa
     3361 aataaagcta catgcttgga atagtaaccg taaatttcga tcccttcccc actccgtcag
     3421 attcaattgc gatgtctcct ttatgggcaa gtattatttg ttttgccaag tacagtccca
     3481 acccagaacc accagtgttt aatctaccgc cctcgccacg gctaaactta tcaaacaact
     3541 ttgcttttgt ttcgggtgat atgccaatgc cgttatcagc aacttgatac acaatagtat
     3601 taccttcacg ccgaagcgag agttttacaa acccatgcgg cgtgtactta atgctgttat
     3661 ctgctaagtt caaaaatgcc tgtttcagct tcagccggtc gacatgtgcc ataaacgatt
     3721 gattacttgg aatatcaatg gtaaattcga gccctttatc ttcagcggta atgcgtattt
     3781 cgttgtacaa atcagtaagc aactttccaa ggtccgtttc ttcaaactca aacttcaatc
     3841 ctccttgttc aatttttgaa atattcaaca aatcttcaac caaactcgaa agcccttgtg
     3901 ctgaaacata aatacgttta attgcacctg cctgttcagt ggtgagtttt ccgtacgctt
     3961 cttcttcaag cattgaggca taccccttta tggcagtcag cggcgcccgt agctgatgcg
     4021 atgcgagcga gagaaattct gtttttagtt tatccaaatc ttttagttta ctgttagcaa
     4081 cctctaaatc tccattgagc tgctcaactt ttttatacag tgttgctaat tcaaaatttt
     4141 gcttgtacaa ctcttcagtt gctttttgta attcattaac ggtaactcgg ttagtatcag
     4201 caggatcagg catatatgat tatcgaagac ttgaaggtat ttgatccaac ggaagtgttg
     4261 cgattaaatc ttttgttgcc tctttgcaca cttcgtgtga agcacgacca aacaaccgct
     4321 cgtactgcgc aacaagctta tcgatagttt ctttcccatc accccttagt gaaatttttc
     4381 cttctttaga attagcaata acgccaggaa ctttttgagc ttcaaaccat gcaacaggac
     4441 cgataatgag ttcttgttct ttgataatcc gtattgaaat ttgatcaaag atagtcataa
     4501 atttactgta ctgtatgaac tgaaaccttt tcagatttta tatgattaca aaaaccaacc
     4561 actccaaagg ctaagaagaa aacccctaaa gtaaggataa ggtctccgaa gtttgcattg
     4621 taataggtgc ccgcagtggt agtatatgaa aaaatagagt ctgctataaa cattactgct
     4681 aaccccaata atattgatgc aatatctaat atatatctgc cgcctaagta tttaaatgaa
     4741 agccctgaaa taactacaga aacagcaaga ccaataaaat ctccgaccgg atatgcgata
     4801 tctagtattg tttttaagag tgattccccc tcgggaacaa gcactccacc ccgtgctaca
     4861 gtaacaagaa cgtaataaga aaatacaaaa atagcaattg gtgcaaggta ggcaaaaatt
     4921 tttgctccgg tgcttcgtaa tccaaatttt gcaccagttg ctttcgctaa atatattgaa
     4981 ccaagcccat agaagaaaat gcttggggca aagcctagat cagcaagtga cgggtatggt
     5041 gcaggtactc cttcaaagaa attatagtac gaccaaattg cctcaccgca accccataaa
     5101 aaaataccaa aactgataaa aaagagtgcc tttcccaccg cacttttaag tccaccccat
     5161 gactgagcac ctctcatgcc aataatgcca ccgatgagcg gaatgagtcc gaaaagaaaa
     5221 gagtagagat agttataaaa accttctttt atatcgctta aaaacaaaac tgcccagaat
     5281 acaagtatta gtgctaaaaa tgtgaggaaa aatttttgat aggtatttat tttcatacct
     5341 tggctgcacc ctaattatac tttttgtaat aaaagacgtc aaataaagta gacttttggt
     5401 tttggaaatc cgttgaatgg gcatgttgct acaagactat aggcgccgac atcatggatg
     5461 attaatttat cgccaattgc aacatcagcc ggaagaaaca cttctcgagt gataacatca
     5521 gggctatcac ctgttggtcc tgcaagggca aacggagctt ccccaacatc aaatgaaggt
     5581 cgaagtaacg tggtacggta ccgtgttgac ccttggtaca ccattgactc aaataaaccg
     5641 ctatacactc ctgcatctaa gaatagccaa gtatgttcac ggcgttcaat tcgagcaata
     5701 acatttgtta ccaaaattcc cgtctctgca actatagctc tgcctggctc aagtaaaagt
     5761 tttggctgat acggaaggtt tttatactcc tgcagtgtgt gcgctgcaat ttcttgaagc
     5821 gtaaagtcat tttcagttga agtatattta ttacacggaa atccgccacc gatatcaata
     5881 acctcaagtt ttatatctgc ttcgagcagc ttttcaagtg ttggtcgaag taaagcaatt
     5941 gcttctgccc aggcacgttc attccctgcc tgtgaaccta cgtggaagct gatgccatac
     6001 ggcactaacc ccaactctcg tgctcgctct agcattggca gtgcattttc ctttgcggtt
     6061 ccaaatttct ctgaaaactt aaaaacactt cctgtatcat caacgcttat acgagcatac
     6121 acccgagcac caggaacaag tgcggcaatt ttttcaagct ctaaaaaaga atcaaacgca
     6181 aatcgatcaa caccataagc agcaaattct ctgatatgaa atggtggttt tactgaggtt
     6241 ccatacacaa ttctttctcc agggacacca acttccttca aagtttcaag ttcatagact
     6301 gatgccacct caaaatgagc acctgcatca aaaagctctc tgagtatttc aggttcagca
     6361 tttgccttca tcgcatagtg aatagccgct cccggaaagc tttcttcaaa ctctttgtat
     6421 tttgcgagta atcgctcttt tgaaaaaaga aaaaaaggag tttcctgatt atatatctca
     6481 aggatttcac gggtaaacgg attctgagga gattcggtat taggcataaa agtatataaa
     6541 ttgtaagtat ctaattataa tacagacagg agaattatcc caatgacaat aacaaaaccc
     6601 ccgattgttt gtgttactgt cacccgctcc ttaaggaacg agcgggcgag aaaaatggta
     6661 ggaagtgaaa atgctccact caacaatgct ataacacttg ttttagtcgt cgcgctatac
     6721 cccaaactaa ttgcaagata cgcaactgtt tcgcagaatc ctatcagcaa taaaaatttc
     6781 catatcggtt tttttataac acacaagtct acttttttca ctttcgcgaa aagtaaaata
     6841 gcaaccgtca taaacgcgta cataagaaag ctatagatca cccaatctgt tccaccgaca
     6901 accttgtccc agccgaccgt ccaaacagct gcaagtgcgg ttgcagcagc aatttctttg
     6961 aaaccaggaa cctgtacaaa attaaatttt ccgagatgta attttgaaaa atcagtatta
     7021 attaatagta cgccaccaaa aattataacc agagcaataa caagattact actcaccact
     7081 tcaccaaaac caacaatcga aataagggct gcaataccag aaaaagatgc aaataccgga
     7141 ttaaggatag aaacttgccc tttaccaaac ccgcgatata ccagaatata aataatagcc
     7201 tgcagcacgc caaaaaaaat caaatacagc agtgaactac ctggatttaa aaacacaatc
     7261 ggacgaccaa gtacgagaaa cttataaacc gcaataatga aaaagaaccc tgttccaaac
     7321 acatgtgccc atgccagact ctgtatatca ccaacttcat ctatagtttt ctttgcaaaa
     7381 aaatcagcaa gcccccatcc gagcattccc cccaatccgg caagtagcgc aatgattaat
     7441 tcttggtgca tattttaccc cttcggcttc aagagcggaa agagctgtgc ttcacggacg
     7501 gttttattgg caaggaaggc gaatacgcgc tcgctcatgc caaacccgca cgttggtggc
     7561 atgccgtact ctaaggcttc aacaaactca gtatcaaaca
//
LOCUS       DATIBQ010000094         3147 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U16294
            k141_2059342, whole genome shotgun sequence.
ACCESSION   DATIBQ010000094 DATIBQ010000000
VERSION     DATIBQ010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935789
            Sequence Read Archive: SRR25158331
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:58:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 637
            CDSs (total)                      :: 603
            Genes (coding)                    :: 601
            CDSs (with protein)               :: 601
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3147
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2059342"
                     /isolate="SMAG_U16294"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..794
                     /locus_tag="VLB02_03170"
     CDS             <1..794
                     /locus_tag="VLB02_03170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE57059.1"
                     /translation="MDCVILSLHMKRFVLVLFFLLLIAPLGAFAQTGIPASSFWYTPT
                     SFKAGDTVTFTVLVYNGEPYETAFTVLLQDGTKKIEEQQVILAKNTAMPVRFSWKAAD
                     GAHAFTATIASARSNGKVLTVASAQKNAVLQTGAAATSTEGKITEIQGVVKSKAGTIL
                     AAIDSWRVKKLAVFTARKAALKSTIDSTDATEKLNSIADSTTSEQEDIVPTQKAFLPN
                     ISPLVYLEFFWYTVLVFVFSSALIFYITTGLAAFLVLKFLYARFV"
     gene            813..1115
                     /locus_tag="VLB02_03175"
     CDS             813..1115
                     /locus_tag="VLB02_03175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE57060.1"
                     /translation="MSLFQRGWYPWFVGVLALGAVLFFPFWVQVLAITLVLTTVRPAA
                     LALIPALAADLLYSGSAVLWKNFVMTGVVCAALAVYWLLKTKTRIFYAYDSMEKKS"
     gene            1090..2793
                     /locus_tag="VLB02_03180"
     CDS             1090..2793
                     /locus_tag="VLB02_03180"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HSE57061.1"
                     /translation="MIRWRKSRKQRGFELNPDEILLDAHNLPAFNTQQFEGRVERPIR
                     KQSLRLLFLITLAFSLIFVGKLVQIQLVRGAYFAEKSDQNSLEHTLIFAGRGIIYDRS
                     GVELAWNDTTDPERGPRRSYISQGGFASLLGYVSYPTKDSAGKFWQEYIAGKDGLEKE
                     YNELLSGKNGVKLVETNVKGELLAENVIEAPLEGKNLTLALDAGVQEELYRGITALAA
                     QSGYRGGAGAIMDIHTGELLALTSFPQYDQAILSDGTNKTAIASYLKSSEKPFLNRTL
                     NGLYTPGSIVKPFLALGALQEGTITPEKIIVSTGKLVIPNPYNLGLESVFKDNKAHGP
                     VDMRRALAVSSNVYFYQLGGGFGSQQGLGIANIEKYATLFGIGKKTGIDISGEKEGNI
                     PSIAWKEKRFPGDPWRIGDTYNTSIGQYGFQVTPLQMLRAVAGVASRGTLVTPRTVLL
                     SASENSEKKPETVSLPIKDEVFTVVQEGMRLCVTEGTCTALQMSEMAIAAKTGTAQIK
                     QNTRVNSWVIGFFPYEAPQYAFVVLMEDGPLISSGAAHAIKPVFSWMRANRPELLTLP
                     Q"
     gene            2829..>3147
                     /locus_tag="VLB02_03185"
     CDS             2829..>3147
                     /locus_tag="VLB02_03185"
                     /inference="COORDINATES: protein motif:HMM:NF015414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HSE57062.1"
                     /translation="MKEYLTQENMQSLEAELKNLSSVKRREILSALEYAKSLGDLSEN
                     AEYHQAREEQRRLEERISQIEHVLKNAVVVKKAQTDKVTLGSLVTIQKKGEGNTRQFH
                     LVGS"
BASE COUNT          807 a          689 c          839 g          812 t
ORIGIN      
        1 taatggattg tgttatactt tcgctacata tgaagcgttt tgtattagta cttttcttcc
       61 tactccttat agctccactc ggtgcgtttg ctcaaacagg tattccggcg agttcgtttt
      121 ggtacacccc tacatctttt aaagcggggg acactgtcac gtttacggtg ctcgtatata
      181 atggtgagcc atacgagact gcatttactg ttttgctcca agacggtaca aaaaagattg
      241 aggagcagca ggttattttg gcaaaaaata ctgcgatgcc ggtccgtttt tcgtggaaag
      301 cagctgacgg cgcgcatgct tttacggcaa ctattgcttc tgctcggagc aatggaaaag
      361 tactgactgt tgcttcggca cagaaaaatg ccgtactcca aacgggagca gcagcaacta
      421 gtactgaagg aaaaattaca gaaattcaag gggtagtaaa gagcaaagca ggaacaatac
      481 ttgccgctat tgatagctgg cgggtgaaaa agcttgcagt atttaccgca cgtaaagcag
      541 cgctaaaaag tactattgat agcaccgatg ctacagagaa gctcaattca attgctgatt
      601 ctactaccag tgaacaggag gatattgttc cgacacagaa agcattttta ccgaatattt
      661 ctccgctcgt gtacctcgaa tttttttggt acaccgtgct agtgttcgtg ttctcttcgg
      721 cgctcatttt ttatattact accgggcttg ctgcattttt ggtgctcaag tttttgtatg
      781 cgcggtttgt gtaggagtgg tattattcgg gcatgagttt gtttcagcgg ggctggtatc
      841 cgtggtttgt tggggtgctt gcactcggtg cagtgctctt ttttccgttt tgggtacagg
      901 tacttgctat tactctggta cttactacgg tgcgaccggc agcactggcg cttatcccgg
      961 cacttgctgc cgatctgctc tacagcggct cagcggtgct ctggaaaaat tttgtaatga
     1021 ctggagttgt atgcgcagcg ctggcggtat actggctgct taaaacaaaa acgaggattt
     1081 tttatgcgta tgattcgatg gagaaaaagt cgtaaacagc gagggtttga gctcaaccct
     1141 gatgaaattc tgctggatgc gcacaatctg ccggcattta atacgcagca gtttgaaggg
     1201 cgggtagagc gtccgattcg aaaacaatcg cttcggctgc tatttttaat cacactcgct
     1261 ttttcactta tttttgtcgg caaactggtg cagattcaat tagtgcgcgg tgcatacttt
     1321 gcagaaaaga gcgatcaaaa tagtttggaa cacacgctta tttttgccgg gcgcggtatc
     1381 atctatgatc gcagcggagt ggagcttgcc tggaacgata ctaccgatcc cgagcgcgga
     1441 ccgcggcgca gctatatcag ccagggcggg tttgcgagcc tgctcggcta tgtgtcgtac
     1501 cccacaaaag acagtgccgg aaaattttgg caggagtaca ttgctggcaa ggatggtttg
     1561 gaaaaagagt acaacgaact gctctcaggt aagaacgggg tgaagctggt tgaaacaaac
     1621 gtaaaaggag aactgttagc tgaaaatgtg attgaagcac cgctcgaagg aaaaaatctc
     1681 accctcgccc ttgatgcagg ggtgcaggaa gaattgtacc gggggattac agcactggct
     1741 gcacagtcgg ggtaccgggg cggtgccggt gccattatgg atattcatac cggcgagctt
     1801 ttggcactca ccagctttcc gcagtacgac caggcgattc tttcagacgg aacaaacaaa
     1861 actgcaattg caagttattt aaagagttct gaaaagccat ttttgaatcg taccttaaat
     1921 gggttatata ccccaggctc gattgtaaag ccatttttag cgcttggtgc gctgcaagaa
     1981 ggaacaatta ctcccgaaaa aataattgtt agtaccggca agttggtgat tccaaatccg
     2041 tataatctcg ggctcgagtc ggtatttaag gataacaaag cacatggacc ggttgatatg
     2101 cgccgggcgc tcgcggtgtc ttcaaacgtg tacttctacc agctcggcgg ggggtttggt
     2161 agtcagcaag gactcggcat tgccaacatt gaaaagtacg cgacactgtt tggtattggt
     2221 aaaaaaaccg gtatcgatat ttcaggcgag aaagagggga atattccgag cattgcttgg
     2281 aaggaaaagc gttttccggg tgatccgtgg cgtattgggg acacctacaa cacttctatt
     2341 gggcagtacg ggtttcaggt gacaccgctc caaatgcttc gggcagttgc tggtgttgct
     2401 tcgcggggca ccttagtaac accgcgcacc gtactccttt ctgcttccga aaattctgaa
     2461 aaaaagcctg aaacagtatc cttacccatt aaagacgaag tctttaccgt agtacaagag
     2521 gggatgcgtc tgtgtgttac tgaaggaacc tgcaccgcgc tccaaatgtc cgagatggcg
     2581 attgccgcaa aaactggtac ggcgcaaatt aagcagaata ctcgggtgaa ctcgtgggtc
     2641 atcggcttct ttccgtacga agcaccccag tatgcgtttg tggtgctgat ggaagatgga
     2701 ccactcattt catcgggcgc ggcacatgct attaaaccgg tgttttcgtg gatgcgcgcc
     2761 aaccggccgg aacttttgac ccttccacag tagtgcggta tagtgttccg atagtaatag
     2821 ggtaacgtat gaaggaatat ctgacgcaag aaaatatgca gtcgctcgag gcagagctga
     2881 agaatttgag ctcggtaaag cggcgcgaaa tcctctcggc gctcgagtac gcaaagtcgc
     2941 tcggcgacct ctctgaaaat gccgagtatc accaggcgcg cgaggagcag cgccggcttg
     3001 aggaacgcat ctctcaaatt gagcacgtgc ttaaaaacgc agtggtggta aaaaaggcac
     3061 agaccgataa ggtaacactc ggttcgctgg ttactattca aaaaaagggc gaaggaaaca
     3121 cccgccaatt tcatctggtg gggagcg
//
