LOCUS       DATIDL010000001         8235 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrosopumilaceae archaeon isolate SMAG_U16342
            k141_2825761, whole genome shotgun sequence.
ACCESSION   DATIDL010000001 DATIDL010000000
VERSION     DATIDL010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935836
            Sequence Read Archive: SRR25158326
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosopumilaceae archaeon (soil metagenome)
  ORGANISM  Nitrosopumilaceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrosopumilales;
            Nitrosopumilaceae.
REFERENCE   1  (bases 1 to 8235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8235)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/04/2024 05:09:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,520
            CDSs (total)                 :: 1,474
            Genes (coding)               :: 1,474
            CDSs (with protein)          :: 1,474
            Genes (RNA)                  :: 46
            rRNAs                        :: 1 (5S)
            complete rRNAs               :: 1 (5S)
            tRNAs                        :: 43
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8235
                     /organism="Nitrosopumilaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2825761"
                     /isolate="SMAG_U16342"
                     /isolation_source="soil"
                     /db_xref="taxon:2202732"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..264
                     /locus_tag="VLB45_00005"
     CDS             <1..264
                     /locus_tag="VLB45_00005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04120.1"
                     /translation="KMKITIHNELEKGTSLGRVVLPVTDHELYYRDFNARIDNLKVIM
                     KNRSGLELAEIIQEKISKTEGWQSAPTDMQKTDYKFSMEKGFD"
     gene            265..1098
                     /locus_tag="VLB45_00010"
     CDS             265..1098
                     /locus_tag="VLB45_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HSD04121.1"
                     /translation="MAIELLGNLSTINVLGNEISIGSLILGAIIMIVGVIVARIASML
                     FKRYFAANLEEDTAKSLRKTIYYGIIIVTLLAVTTSQGIDLSGLMVAGGIFGIVIGFA
                     TQSVVSNLISGIFLMFEKPAKTGDLIEIPSNKVYGRLIDITIFSTRIKLFDGSIMRVP
                     NEKVFTSEIRNVSGSEVRRIDGVVGIAYKEDIDKAIEVIKEEIGKLPYVLCEPEPAIW
                     TEELADSSVNLKILTWLPRDNWDEVGPVLLKVTKQALDKAGIEIPFPQRRIWHAENSR
                     S"
     gene            complement(1108..1506)
                     /locus_tag="VLB45_00015"
     CDS             complement(1108..1506)
                     /locus_tag="VLB45_00015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6659 family protein"
                     /protein_id="HSD04122.1"
                     /translation="MKQSFKELCEKIVTLDPTIRFVGIADEDGTLKADAERKGLKAVF
                     SPEQRAEYAITAANRQTTRLRWEYLLGKIQYASSHYEKLIRSTIPIADENRRLSHVIL
                     VSFDTNADVHNIITKKIIPLVRQNIKDLQN"
     gene            1632..1808
                     /locus_tag="VLB45_00020"
     CDS             1632..1808
                     /locus_tag="VLB45_00020"
                     /inference="COORDINATES: protein motif:HMM:NF018096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1059 domain-containing protein"
                     /protein_id="HSD04123.1"
                     /translation="MTKKFACGDVVNGCAWSATAKDESELFSKIAEHAKKDHGITEIP
                     QDLIEKVRSKIQTI"
     gene            complement(1840..2463)
                     /locus_tag="VLB45_00025"
     CDS             complement(1840..2463)
                     /locus_tag="VLB45_00025"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HSD04124.1"
                     /translation="MGKYVVPPLNYSYDALEPYIDAKTMEIHHTKHHQAYANGLNAVL
                     ESINALTHQNYIVSVLSDLTAVPESARNAINFHGGGFENHRLFWENMKPNGGGEPGGR
                     LADEISIYFDSFKKFKEEFSRGTISIQGSGWGWLVYNLTYSRLEFRTMPDQTSPWTQR
                     LVPLLGLDVWEHAYYLKYQNRRADYVEAWWNVVNWNEVEERFARVST"
     gene            complement(2681..3064)
                     /locus_tag="VLB45_00030"
     CDS             complement(2681..3064)
                     /locus_tag="VLB45_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010191849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CrcB family protein"
                     /protein_id="HSD04125.1"
                     /translation="MKGIEIILLAVGGVIGTFLRYKIVESPLLFGALQINVLIVNIIG
                     SFILGIFVVATQQWQIDSKYALLVAFGFCGSLTTMSALALDTTNILYNRQFELAAINV
                     AANVGLSIGAIFAGKTLLEVVIKLT"
     gene            complement(3223..3567)
                     /locus_tag="VLB45_00035"
     CDS             complement(3223..3567)
                     /locus_tag="VLB45_00035"
                     /inference="COORDINATES: protein motif:HMM:NF012753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HSD04126.1"
                     /translation="MKRIEAIVRPGKVRLVLAALKDVGSRAVTVVVAKGQGKGARPLV
                     RDNRGTTLFTAEYNAMNSIITIVDDAMVNDVISAIAKAVGEGSKASGKIFISPVEDVI
                     DLESGRRGPSAL"
     gene            3703..3852
                     /locus_tag="VLB45_00040"
     CDS             3703..3852
                     /locus_tag="VLB45_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016940217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04127.1"
                     /translation="MKTIGLQSKPSTWKREVRASFRKIVAFDLLFYGLGILTGAGIMA
                     AILLA"
     gene            complement(3893..4177)
                     /locus_tag="VLB45_00045"
     CDS             complement(3893..4177)
                     /locus_tag="VLB45_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04128.1"
                     /translation="MEPMMTISTIVSGLNMIVLGIVIGVFAKMYAKTRAQLPIGMIFF
                     AGLLFLHNVIGVYAYFSMMELYAVALLPYLLAVHIAEFAGILIFLKITLQ"
     gene            complement(4203..4505)
                     /locus_tag="VLB45_00050"
     CDS             complement(4203..4505)
                     /locus_tag="VLB45_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016939535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HSD04129.1"
                     /translation="MAGDPYAKRLLWFVFAGSKGGLNRIRLISTIRKTPLNANQLAKE
                     LSLDYKAIQHHVNVLEKNNLITRMGDKYGVTYFISTFLEVNLESFDEIVTKLEKGK"
     gene            complement(4555..4965)
                     /locus_tag="VLB45_00055"
     CDS             complement(4555..4965)
                     /locus_tag="VLB45_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012216043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HSD04130.1"
                     /translation="MKEQELKLISDQMKCCPVDNTFKIIGKKFTLHILRNMTMHNQNR
                     FNQFLDSIEGINPKTLSVRLREMERNGLISKKIYAETPVRIEYTITEKGKALKPIVEQ
                     MAAFSMKYCCQDIFKDGKSRTFKEVFGRPIKELG"
     gene            5053..5394
                     /locus_tag="VLB45_00060"
     CDS             5053..5394
                     /locus_tag="VLB45_00060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04131.1"
                     /translation="MECTTESCNMEKNATCACGSEGSCEECCDCQSVDPIEQSMAMWH
                     KAFFQALHEVQVERLKKRIESGFGPTMDKSADAVFEAISKVWSSMLTQTEAKKELASK
                     LQKIFSETSRK"
     gene            complement(5443..6168)
                     /locus_tag="VLB45_00065"
     CDS             complement(5443..6168)
                     /locus_tag="VLB45_00065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04132.1"
                     /translation="MLILQDPFVEKIHKHLESVGMKTEILPSGSMDFEYDNPRSSLSG
                     PSQGIASMGSMRVLDGPITYLEVLKRKILEKSDFAPGGSYGAGVYEYTTWKFRFFISL
                     PATINLGPLNIGTITKILKGKFHSKVEDFVWNGYGKLTTLPPGIVHDDMISVLNSDAI
                     LHDLMIKSLLKEKTISIHAYSPKKTREQTGGYQSFAKVAISSDWKPQHDMLIDKNTVE
                     TYQKIASDIRRTIITLRYVLENN"
     gene            complement(6452..6928)
                     /locus_tag="VLB45_00070"
     CDS             complement(6452..6928)
                     /locus_tag="VLB45_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04133.1"
                     /translation="MNNLALKLVFSNRRYLILSTAIFLGMILSLSIISEFLFLEPYVI
                     FYIPDYRIVSFLLLVIVSALSGLVISMSVYRILLLHTTRISSGFVGSIIGASAGACSC
                     GSVGFAIASTFGAVGGAATAFLTNYEIPLRVISIGILVYSYYISAKGLSAQCKVQK"
     gene            complement(6993..>8235)
                     /locus_tag="VLB45_00075"
     CDS             complement(6993..>8235)
                     /locus_tag="VLB45_00075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04134.1"
                     /translation="SAMEMKNSMMGKLTNTSVPVTIPLTKGYVKGSEVFYISTEASDK
                     DLADHLTKLTGFRVAYTPALQKTPADALAQIYAFENGIEGQGPLGFQPNVADSQPGDL
                     NYSPLWAINIVKWTDGSTPRELKSQTEILAAQTNGELTITPSGFIVNCPFVQWDGGSL
                     QIRDDKTLTDETPYGGGQVLEINIENMQVTFVAHRGFGPDGSTIYYIATDASKQDVAD
                     MLGVVFAERTGTTLLSGASSDLYVFTNGIKGSGPMGFQASIGSTNVGDEFYSPLWRIQ
                     AATWTDPSLADFLTMTSQITSAGSNGALTTDIAGVVVNCPFIEVDEKFMMDTTMIKNN
                     MMEHIASPRTQMKMGVDVHEIQCKSGHELVFKLTNWSPACVKSSSVARLVEIGWASNH
                     IPSEDEMMKKDSMMEDKMMKK"
BASE COUNT         2511 a         1502 c         1513 g         2709 t
ORIGIN      
        1 aagatgaaaa tcaccatcca taatgagtta gaaaaaggaa catctttggg cagagtagtt
       61 ttacctgtta ctgatcatga actttattat cgcgatttca atgccagaat tgacaatttg
      121 aaggtcatta tgaaaaatag atcgggatta gagttggctg aaataattca agaaaaaata
      181 tcaaaaacgg agggatggca aagtgctcct actgatatgc aaaaaacaga ttacaaattt
      241 tcaatggaaa agggttttga ttaaatggca atagaattac ttggaaatct gtctaccata
      301 aacgtcttgg gtaatgagat ctctatcgga tctttgatcc ttggtgcaat aattatgata
      361 gtgggagtga tcgttgccag aatagcaagt atgcttttta aaaggtactt tgctgccaat
      421 ttggaagaag atactgcgaa gagcttgcgc aagactatct attatgggat aatcatagta
      481 acactccttg cagttactac cagtcaaggc attgatttaa gcggcctaat ggttgcagga
      541 ggaatatttg gcatagtcat aggctttgcc acccaatcag tggtatcaaa tcttatatca
      601 ggaatttttt tgatgtttga aaagcctgca aaaactggag atcttattga aattccaagc
      661 aacaaagtat atggaagact aatcgacatc acaatttttt ctacgcgaat aaagttgttc
      721 gatggttcca taatgcgtgt acctaatgaa aaggtcttca catcagaaat cagaaacgtt
      781 tctggaagtg aagtaagaag aattgatgga gtggtaggga ttgcctataa agaagacata
      841 gacaaagcaa ttgaagtaat caaagaagag attggaaaac ttccttatgt attatgtgaa
      901 ccagaaccag caatatggac tgaagagctt gcagattcta gtgttaatct aaaaatacta
      961 acttggcttc cgcgcgacaa ctgggatgaa gttggtccag tattattaaa agtcacaaaa
     1021 caagcgcttg ataaggcagg aattgaaata ccattcccgc aaagaagaat atggcacgct
     1081 gaaaattcaa gatcctaaac tagggatcta attttgaaga tctttgatat tttgacgaac
     1141 caatggaatt attttttttg ttattatatt atggacatcc gcgtttgtat caaatgacac
     1201 caatattaca tgtgagaggc gtctattttc atctgcgatt ggaatagtgg aacgaattag
     1261 tttctcatag tgcgagcttg catattgtat cttccccaaa agatattccc atcgtaatct
     1321 tgtagtctgt ctgtttgcag cagtgattgc atattctgct ctttgttctg gagaaaacac
     1381 ggccttgagt cctttacgtt ctgcatcggc tttcaaagta ccatcttcat ctgcaatacc
     1441 tacaaaccta atagttggat ctagtgtaac aattttttca cacaattctt tgaaagattg
     1501 cttcatcaga ccaagatatg gcgaactagg tatatgtcat ttccgatttt atagaaaatt
     1561 gattttacta atttagaagt agattttttt gataggaata atgttaaatt cttgtacatt
     1621 actaaagata tatgacaaaa aaatttgctt gcggggacgt tgtaaatggt tgtgcatggt
     1681 cagcaactgc caaagatgaa agtgaattat ttagtaaaat agctgaacac gccaagaagg
     1741 atcatggaat aactgaaatt ccacaggatt tgatagaaaa agtacgatca aaaattcaaa
     1801 ccatataatt ggttttttat agatcaatca aaaatggatt tatgtagaca ctcgtgcaaa
     1861 tctttcttcc acttcattcc agttcactac attccaccaa gcttcaacat aatctgcacg
     1921 acggttttga tattttagat agtaagcatg ttcccaaaca tcaagcccca acaagggaac
     1981 tagtctttgt gtccaaggac tggtttggtc aggcatcgtt ctaaattcaa gtctagaata
     2041 tgtcagatta taaacaagcc aaccccatcc gcttccttga atagatatcg tgccacgaga
     2101 gaattcttct ttaaactttt taaaactgtc aaagtagatg ctgatttcat cagcgagtct
     2161 tccgccaggt tcacctcctc catttggctt catgttttcc caaaacaatc ggtggttttc
     2221 aaatccacca ccatgaaagt ttatggcatt acgagcgctt tctggaactg cagttagatc
     2281 agagagaact gatacgatat agttttgatg ggttagtgcg ttgatgctct caagtacagc
     2341 atttagcccg tttgcatatg cttggtgatg ttttgtgtgg tgtatttcca ttgttttagc
     2401 atcaatgtat ggttctagtg catcatagga ataattcaat ggcggaacta cgtattttcc
     2461 cataagatat gattttgaaa ttctgtcctt atatcccaat tgattgtttt atgtagctag
     2521 ttctattttt cttggagaat gttttgagat gtctttgtgg atgatagcat gtgtttcatg
     2581 tttgaatgtg agatcacatc tgtaacattt ccatgctgtc atgctcatgg atttagttta
     2641 gcaatttttg tatataaaat ccgcgtagct tttgtacttt tcaagttagt tttatcacaa
     2701 cttcaagtag agtttttcct gcaaatattg cacctattga taatccaaca tttgcagcca
     2761 cattgattgc tgcaagctca aactgtcgat tgtacaaaat attggtagta tccaatgcaa
     2821 gtgcagacat ggtagtaagt gaaccacaaa aaccaaatgc cacaagtaat gcatacttac
     2881 tgtcaatttg ccattgttgt gtagctacaa caaagattcc aaggataaaa ctgccaatga
     2941 tgtttactat aagcacatta atttgcaagg caccaaaaag caatggcgat tctactattt
     3001 tataacgtag aaatgtgcct atcacgccac ccactgcaag aagaattatt tctattcctt
     3061 tcatttgtct accaatttcc aatttttcta gtagacatta tcagagtcat gcgacacggt
     3121 tctggctttt acaccaaggc taatgtcata gcctgattct cttttttcat atacaatttt
     3181 taaagttttg gattaatgat gctagtttag aaatttgtat ggctataatg cacttggacc
     3241 tctgcgtcct gattctaaat ctattacgtc ttcaactggg gagataaaga tctttccact
     3301 tgctttagaa ccttcgccaa ccgcctttgc tattgcagat atcacatcat ttaccatagc
     3361 atcatctact attgtgataa tagaattcat cgcattgtat tctgcagtga agagcgtagt
     3421 accgcgatta tctctaacta ggggtcgtgc tccctttcct tgaccctttg caacaacaac
     3481 tgtgacggct ctgctgccaa cgtctttgag tgcagccaac accaacctta cctttccagg
     3541 tctaactatg gcttcgattc ttttcaacta ttatcttaaa ttttgacttg tataaattga
     3601 ttttaaaaca tgcgtgttaa ggctttatcc ttaaacaaaa tagcttaatc ttcataattt
     3661 gagctgaata gaatctatga acttactcca gtaattacag gtatgaaaac aattggacta
     3721 caaagcaagc caagcacttg gaaaagagag gtacgtgctt cttttagaaa gatagttgcc
     3781 tttgatcttc ttttctacgg tctaggcata ctcacaggtg ctggcatcat ggcagcaatt
     3841 ctacttgcgt agattgctac caattttttt atttttgatt taaatcacat atctattgca
     3901 gtgttatttt taaaaatatc agtatgccag caaattctgc aatatgaact gctaaaagat
     3961 atggtaaaag agccaccgcg taaagttcca tcattgaaaa gtatgcatat actccaatga
     4021 cattatgcaa aaataatagc cctgcaaaaa atatcattcc tataggcaat tgggcccttg
     4081 ttttagcata cattttggca aagactccga tgactatacc taacacaatc atgtttaggc
     4141 cagatactat ggtactgatt gtcatcattg gttccatgta agaaaaatcc actctcccaa
     4201 gcttatttac ctttttccaa ttttgttaca atttcatcaa atgattccag attaacctca
     4261 agaaacgtag aaatgaagta agtcacacca tatttgtcgc ccattcttgt tatcaagtta
     4321 tttttttcaa gaacatttac gtgatgttgt attgctttat agtctaaact aagttccttg
     4381 gcaagctgat ttgcattaag cggagttttt cttattgtgg agataagacg tattctgtta
     4441 agaccgcctt ttgatcctgc aaaaacaaac cacaaaagac gttttgcata aggatcgcct
     4501 gccatgtttt gtatcagatt cgagtgtatt ttataattaa aaggtgttat gaaactagcc
     4561 taattccttt attggtctgc caaagacttc tttgaaagtt ctggatttac catctttgaa
     4621 gatatcctgg caacaatatt tcattgaaaa tgcagccatc tgttcaacta tgggttttaa
     4681 tgccttgcct ttttctgtaa ttgtgtattc aattctaact ggagtttctg catagatttt
     4741 ttttgatata agaccgtttc tttccatttc acgcagtcta acagaaagtg ttttgggatt
     4801 tattccttca attgaatcaa gaaattgatt aaatctattt tggttatgca tcgtcatgtt
     4861 tcttaaaatg tgcagtgtga attttttacc tataattttg aaagtgttat caaccggaca
     4921 acacttcatt tgatcagata tgagttttag ttcttgttct ttcaacttac tatcttctca
     4981 cttactttcg ttgtagttac tttccaattt atatacttac ctttctatag ttagtagcat
     5041 aaggaaagta atatggaatg cacaacagaa tcatgtaaca tggaaaaaaa tgcaacgtgc
     5101 gcatgtggct ctgagggatc atgtgaggaa tgctgtgatt gtcaaagcgt cgatccaata
     5161 gaacaatcta tggctatgtg gcacaaggcg ttctttcaag cactccacga ggtgcaggtt
     5221 gagcgactca aaaagaggat agagtcagga tttggtccta ccatggacaa atctgcagac
     5281 gccgtctttg aagcaataag caaggtctgg agctcaatgc ttacacagac agaggcaaaa
     5341 aaagaattag cgtcaaagtt gcagaagata ttttctgaaa caagtaggaa gtagcaattc
     5401 tccccctgat attcctactt tccctttttt ttgttaaagc aactagttat tttctaagac
     5461 atatctcaaa gtgattattg ttcttcgtat atcagatgct attttttgat atgtttcaac
     5521 tgtgtttttg tcaattaaca tgtcatgttg tggtttccag tcagacgaaa ttgccacttt
     5581 ggcaaacgat tgatagccac ctgtttgttc tctggtcttt tttggagaat atgcatgtat
     5641 tgaaattgtt ttttctttga gaagtgattt tatcattaga tcatgcagta tcgcatctga
     5701 attaagtaca gaaatcatat catcgtgtac gatgcctggt ggaagagtag taagttttcc
     5761 ataaccattc caaacaaaat cttccacctt tgaatggaat tttcctttta gaattttagt
     5821 tatagttccg atatttagtg ggccaagatt tattgtagct ggaagcgaaa tgaagaatct
     5881 aaatttccat gtagtatatt cgtatactcc tgcaccatac gaaccaccag gtgcaaaatc
     5941 acttttttct agaatctttc tttttaatac ttcaagatac gtgattggac catcaagaac
     6001 acgcatagag cccatgcttg cgattccttg acttgggcca gaaagggagc ttcttggatt
     6061 gtcgtattca aaatccattg agccagatgg tagaatttct gttttcattc caactgactc
     6121 tagatgtttg tggatctttt cgacaaatgg atcttgaagt attagcacat aaaatgctat
     6181 caattatgct atataaaatt tatatattaa tttatgattt ataaaaaata tttgtgtatt
     6241 aataatatgc gatctaataa tagcaatata atcttaaata tttgaaaatc taatatatac
     6301 acaatgctcg ttcagtgcag aatgatggat tgaacgtgcg tggcttggca gaaaccagcg
     6361 agtgaatgaa gcgcattggc gcggattcat agagaggtaa tctctcatgt tctgcaacag
     6421 agccacgcat ttcttttgaa atcatggtag atcatttctg aactttacat tgagcactta
     6481 gtccttttgc agatatgtaa tatgaataaa ccaaaattcc aattgaaatc actcgtaatg
     6541 gaatttcata gtttgtcaga aatgcggtag cagctccacc gactgcacca aatgttgaag
     6601 caattgcaaa tccaacagaa ccacaactgc atgcgccagc acttgcacca attatagaac
     6661 caacaaaacc agagctaatt cgtgttgtat gtaacagcaa tattctgtag acgctcatgc
     6721 tgatcaccaa tccagagagg gcagatacaa ttacaagcaa taagaaactt acaattcgat
     6781 aatccgggat gtaaaaaata acatatggtt caaggaaaag aaactcggat attatagaaa
     6841 gtgaaagtat catgcccaaa aatattgcag ttgaaagtat cagatatcgt ctatttgaaa
     6901 aaactagttt tagtgccaga ttattcaagt tagctcatgc atgttttttt agtatttgaa
     6961 aatatcgatg tagtgtgtga tactatattc tgttatttct tcatcatctt gtcttccatc
     7021 atggaatctt ttttcatcat ttcatcttca gatgggatat ggtttgatgc ccaaccaatc
     7081 tcaactagtc tggctacact tgatgatttg acacaggctg gtgaccagtt ggtcaatttg
     7141 aaaacaagtt cgtgtccaga cttgcattga atttcatgaa cgtctacacc cattttcatc
     7201 tgtgttcttg gtgatgcgat gtgttccatc atattgttct ttatcattgt ggtgtccatc
     7261 ataaatttct catccacttc aataaatgga cagtttacaa caacaccagc aatgtcagtg
     7321 gttagcgcac catttgagcc agcagatgtg atttggctgg tcatggtaag aaaatctgcc
     7381 aaagaaggat cagtccaagt tgcagcttga attcgccata gtgggctgta aaattcatcg
     7441 cctacatttg ttgaaccaat gctagcctgg aatcccattg gaccactacc ctttattccg
     7501 tttgtaaata cataaagatc agatgaagcc cctgaaagca aggttgtgcc tgtcctttca
     7561 gcaaatacta cacctaacat atctgccaca tcttgttttg aagcatcagt tgcgatatag
     7621 tatattgtag aaccatctgg tccaaatcct cggtgtgcaa caaatgtaac ttgcatgttt
     7681 tcaatattaa tttccaaaac ttgtcctcca ccatatggag tttcatcagt caatgtcttg
     7741 tcatctcgta tttgcaagct gccaccatcc cattgaacaa agggacagtt tactatgaaa
     7801 ccagatggtg ttattgtgag ttcaccattt gtttgtgctg caagaatttc tgtttgtgat
     7861 tttaattctc ttggtgtaga gccatcagtc cactttacaa tgttgatagc ccatagagga
     7921 ctatagttta gatcacctgg ttgcgaatca gctacgtttg gctggaatcc aagaggacct
     7981 tgaccttcga ttccgttttc aaaagcataa atttgtgcta atgcgtctgc tggagttttt
     8041 tgtagtgctg gagtatatgc aaccctaaaa cctgtaagtt ttgttaaatg atctgcaaga
     8101 tctttatcgg atgcttcagt tgagatgtaa aaaacttcag aacctttaac gtatcctttg
     8161 gttaacggaa ttgtcaccgg tacgctagtg ttagtcagtt tgcccatcat agaattcttc
     8221 atttccatgg cacta
//
LOCUS       DATIDL010000002        17784 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrosopumilaceae archaeon isolate SMAG_U16342
            k141_1615354, whole genome shotgun sequence.
ACCESSION   DATIDL010000002 DATIDL010000000
VERSION     DATIDL010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935836
            Sequence Read Archive: SRR25158326
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosopumilaceae archaeon (soil metagenome)
  ORGANISM  Nitrosopumilaceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrosopumilales;
            Nitrosopumilaceae.
REFERENCE   1  (bases 1 to 17784)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17784)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/04/2024 05:09:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,520
            CDSs (total)                 :: 1,474
            Genes (coding)               :: 1,474
            CDSs (with protein)          :: 1,474
            Genes (RNA)                  :: 46
            rRNAs                        :: 1 (5S)
            complete rRNAs               :: 1 (5S)
            tRNAs                        :: 43
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17784
                     /organism="Nitrosopumilaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1615354"
                     /isolate="SMAG_U16342"
                     /isolation_source="soil"
                     /db_xref="taxon:2202732"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..871)
                     /locus_tag="VLB45_00080"
     CDS             complement(<1..871)
                     /locus_tag="VLB45_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007403162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HSD04135.1"
                     /translation="MLKVLEAYSRDVGRGVARLDYDSTNSLSVATGDIIEIKGKRRTV
                     AKCMPLYPSDEGKEIIRVDGLVRNNLGVAVGDKIEVRKIKAVPAEKIIVAPLDTIPPI
                     DEKYLADSLENTPLVKGDNIMVPYFGGRLTFQVIGITPVADAVIVTSETQFHIGEKND
                     SLHGVPQVSYEDIGGLGTEIKKVREMIELPMRHPEIFEKLGVEAPKGVLLFGVPGTGK
                     TLLAKAVANESNAHFISISGPEIMSKFYGESEARLREIFKEAREKSPTIVFIDEIDSI
                     APKREEVTGEVERR"
     gene            complement(982..1896)
                     /locus_tag="VLB45_00085"
     CDS             complement(982..1896)
                     /locus_tag="VLB45_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007401473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HSD04136.1"
                     /translation="MVLQTLNTGAKCARCGKNSMLTDNATGERFCGGCGFVITERVEE
                     AGPEWRSFSKDEYDDRSRTGSPTSLAMHDMGLATIIGHANKDVTGKPLSASMKSTIER
                     LRTWDSRSQVHESSDKNFRQAFSDLGRLKDKLALSDAVIEKTAYIYRKALEKGLVRGR
                     SIPGLLAAALYAACRDSETPRTLNDVSGEINIKRKDVSRCYRLLLRELDLKMPVVDPI
                     KCMSRIASKAELSEKVKRRALKILTDASKMEITAGKDPMGLAAAALYLSCVMSGENVT
                     QKVIAMAAGVTEVTIRNRYKGLKESLKI"
     gene            2223..2540
                     /locus_tag="VLB45_00090"
     CDS             2223..2540
                     /locus_tag="VLB45_00090"
                     /inference="COORDINATES: protein motif:HMM:NF014047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HSD04137.1"
                     /translation="MSYSGGYGRSNRGYGGGRDRGYGDNRRFNDGPKPVETGKEYDVS
                     IEEISRKGDGIARVDGFVIFVKDGKAGQKAKVKITQVGQRFASAEIVQGSSQSEAAPA
                     ATE"
     gene            complement(2846..3214)
                     /locus_tag="VLB45_00095"
     CDS             complement(2846..3214)
                     /locus_tag="VLB45_00095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04138.1"
                     /translation="MDDEYVRNLLGQISNSYKLLTELRDKPGDLATIKQELGKIHGIL
                     QVLVNKIEKSGNDSDMVSEFLDASKAYLRDYSFNYEIDNVSELYPEDHHRIKNLRTVI
                     LTALEKCEVISKVNTLLQEL"
     gene            complement(3249..3656)
                     /gene="msrB"
                     /locus_tag="VLB45_00100"
     CDS             complement(3249..3656)
                     /gene="msrB"
                     /locus_tag="VLB45_00100"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HSD04139.1"
                     /translation="MSEDDHVLRSDEEWKKHLTEEQYEVCRRKNTERPFSGEYNNCKE
                     KGVYKCVCCGNELFSSETKFNSGTGWPSFFAPISEKNVKSETDTSYGMLRVEVECNKC
                     GAHLGHVFDDGPKPTNLRYCINSVSLKLEKKDQ"
     gene            complement(3698..4261)
                     /locus_tag="VLB45_00105"
     CDS             complement(3698..4261)
                     /locus_tag="VLB45_00105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04140.1"
                     /translation="MAKLKDIVKVIVFLIGGFAIIVVGGFMMRSIAKLWENNDRKKQM
                     DKDPDNNTMMMLSAFLVVLILSTSAHYAQAAIGGYSVNLSGEAWEVQVNKQVQIAADL
                     TNNQIRTQPFAYLVQIQNEEGITISLSWITGILEPGQSMNPSQSWTPTEPGTYNAQIF
                     VWESIDNPDALSAPLTMKLKVVETTNT"
     gene            complement(4286..4639)
                     /locus_tag="VLB45_00110"
     CDS             complement(4286..4639)
                     /locus_tag="VLB45_00110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polyketide cyclase"
                     /protein_id="HSD04141.1"
                     /translation="MPQYFKLLKIRSVRDNIFVVEEHVRIAGRDLVMLTKHVIKPFEL
                     HEVFVLSGDIKGTHIIETFETVPEGTKVTIDANFKVKTMLKLSTSFSKEKIESSMREM
                     VDEVTKIVHRIDDEI"
     gene            complement(4739..5377)
                     /locus_tag="VLB45_00115"
     CDS             complement(4739..5377)
                     /locus_tag="VLB45_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008300497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HSD04142.1"
                     /translation="MPKEQKETRVWPKGYTPAVIPETDSRSNILNVILTSKVPEKHVN
                     TDLFYVMSTRRSTRKFSPRPIEQWKIDKIIAAADTAPTAGNFQGFEIYYVKDPKVKER
                     LVKAANNQPYVNTPIILVFCMNPERVKLNFPQEVLAKFSIQDATLAAAYSQLAASALG
                     LSSIWIGMINEEQVKEAVGTKLRPSSLLCIGYPEQKRGPRSRRKLKDLIHVV"
     gene            complement(5425..5730)
                     /locus_tag="VLB45_00120"
     CDS             complement(5425..5730)
                     /locus_tag="VLB45_00120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04143.1"
                     /translation="MNHSFQIDTPYIPNEKGCRLIWRNDDDEERVIYLRHEDLVHLHE
                     ILSHSSTDKIELEDEVSSIMVNSDTTEFFMAGKEILQIETKTLGEKISEFVKNNPTA"
     gene            complement(5778..6575)
                     /locus_tag="VLB45_00125"
     CDS             complement(5778..6575)
                     /locus_tag="VLB45_00125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04144.1"
                     /translation="MGLLKRSKESEDGRTITDSKNLHKIKVKEIEETQLEATKNELKY
                     TKNELEETIVKVDSASHKLEYIQSEYNAKSNELEKIKAELEKIKPELEEEKIQLSEVR
                     SDYKTTLSELETAKYELALVKSQYDQNEVHSIKQRIETVRIELSGINSQIEYAKSELQ
                     SLLNQQMKKKNEIMTAKKELEFIEKELATVGQRELKFIENELSTVKQKEDTQRVVVAA
                     GSVVASINAKYDAIKKELEIIKMAHSRLKTDYEAVKGELETLRGKTS"
     gene            complement(6673..6870)
                     /locus_tag="VLB45_00130"
     CDS             complement(6673..6870)
                     /locus_tag="VLB45_00130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04145.1"
                     /translation="MAHREHNRPKKNGSYAMILFGAFLFISATLFLPRGSIEGQIAIV
                     FGFIVGGIGFYLGFAKRKKTD"
     gene            complement(6873..8459)
                     /gene="lysS"
                     /locus_tag="VLB45_00135"
     CDS             complement(6873..8459)
                     /gene="lysS"
                     /locus_tag="VLB45_00135"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010194440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0017101 - aminoacyl-tRNA synthetase
                     multienzyme complex [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HSD04146.1"
                     /translation="MEEQKTIGRGTWIDKLAYELVEREKTLGRNTSMIKVESGLGASG
                     IPHIGSLGDAARAYGVKLALENLGYKSELIAYSDDLDGLRKIPEGFPDWLNDHIAKPV
                     SFIPDPFGCHDSYGLHMSSLLLDGLDKCGIKYKFQRGVDTYKNGLLKDQIHTILLNSK
                     KIGEKIAEMVGQEKYQDALPYFPVCSSCGRLYTAEAYEYLPDEKKIKYRCHETTIGSK
                     PLAGCGHDGIADITKDLGKLAWKVEFAARWQAFDVRFEAYGKDIMDSVKVNDWVADEI
                     LKFPPPHHVKYEMFLDKGGKKISKSLGNVVTSQAWLKYGTSKSILLLLYKRITGTREV
                     GFEDIPALMDEYNELENVYFGKIKLENETKTVRAKGLYEYVNLLNVPKTTTAHVNYRL
                     LVELCKIFREQRSELVVKKLIEYGTIKESTAEIEHLISLAGNYADDFGKPSKVEIQLD
                     DATKNAIKELITMLSSENEPVDLQNSIYNIAKAGGIQPKDFFKTLYQIILASDRGPKI
                     GPFILDIGRKKVAQSLSEYI"
     gene            complement(8459..10045)
                     /locus_tag="VLB45_00140"
     CDS             complement(8459..10045)
                     /locus_tag="VLB45_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010194442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine(34) 5-carboxymethylaminomethyl
                     modification radical SAM/GNAT enzyme Elp3"
                     /protein_id="HSD04147.1"
                     /translation="MQKSKILFEEACVEISQNLLAISNPTRNDVKKEIKKACVKYSLE
                     RIPRNYEILAEAHGNDFLKLQKALLRKPIKTASGVAVIALMPKPYACPHGRCTYCPGG
                     VEFNTPNSYTGMEPSTQNAIVNEYDPKKQILDKLKHLQAYGHDGTKLELVIVGGTFLF
                     MPQDYQVNFIKSCYDAINGFDSQDLESAKKNNERAKIRNVGFTIETKPDYCKKTHVDW
                     MLDYGVTRVEIGVQSLHERVYEIVNRGHDLNDVIESFQISKDAGYKIVAHMMPGLPTM
                     TPKEDISDFKKLFEDPAFKPDMLKIYPTLILENTPLYQTYRDGKFMPYSDQDMVEVLT
                     EIKKMIPRWVRIMRVQREISSDQIIAGPKLGNLRQIVQNNLKKQNLSCRCIRCREAGL
                     SESSINVDDIKLNREDYESSGGNEVFLSYDDSSDRIFGFLRLRKPSRLAHRSEVTQNT
                     CIVRELHVYGKSLKLGQRDTDSVQHLGLGKNLMNEAEKISREEFDANRLLVISAVGTR
                     EYYKKIGYSPLGSYMSKELS"
     gene            complement(10079..11101)
                     /gene="purM"
                     /locus_tag="VLB45_00145"
     CDS             complement(10079..11101)
                     /gene="purM"
                     /locus_tag="VLB45_00145"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HSD04148.1"
                     /translation="MVVTYKDAGIDIKKIKQSQATIGRLIASTHKLQKKARIASGFGH
                     YAGIVEIGGGKLVATHTDGVGTKVIISQMMKKYDTIGIDCVAMNVNDIICTGATPISF
                     VDYIAANKNDQEIFKKIVQGLVKGAKKSQVPIVGGETAIMPDVITGKEFSFDLAGMVV
                     GILTKNQMVLGDMITPGDVIIGIKSSGLHSNGYSLARKVLLSKYSIKDKIRGMGILGD
                     ALLEPTEIYVKPVLEALEECDIHGLAHITGGSFTKLLRLKKTGYMLDNMPEPPEIFQL
                     IENQNVEKSEMYKTFNMGIGFCIVAPEEEGEQIDEIFKKHGLKSRQIGIITEKKGVFV
                     DSLRIA"
     gene            complement(11183..11914)
                     /locus_tag="VLB45_00150"
     CDS             complement(11183..11914)
                     /locus_tag="VLB45_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAC2 family protein"
                     /protein_id="HSD04149.1"
                     /translation="MPRNGFPEAEVFEIKKIKLDSPIIFAGFVGAGLAGSIAVGYIIE
                     KLKMKEIGYMRSKYLPPATVFMQGRLRHPFRFYANKGGTICGIICEITLRMEGLYTIA
                     SSILDWAEKNGSKELVVLDGVADKEEHDQKAFCAAEEDLCRVMQDSGINMIPRGFVTG
                     IPGAILNESLIRKIRGVTLLVKANPQRPDTLAAATLVDAVNRAYGTAIDVTELRKQEQ
                     QIGHDFKELSDKYAEARKADSGLYM"
     gene            complement(11937..12281)
                     /locus_tag="VLB45_00155"
     CDS             complement(11937..12281)
                     /locus_tag="VLB45_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04150.1"
                     /translation="MKTRMTYIPLEVAEHFSDFIITRDEQVLDSVKARARDFSTISLL
                     KLLYQLKSSPMTFSDLYMKSNIRMKKSFLNYLHLCVKYSFITKMAVGSNMIYSITDKG
                     RIMLDLFMQKSN"
     gene            12378..12638
                     /locus_tag="VLB45_00160"
     CDS             12378..12638
                     /locus_tag="VLB45_00160"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HSD04151.1"
                     /translation="MPVKELSGMHSAFVLMNAELGKENKLVKELRQIPNVKEVYGVYG
                     VYDVLMEIEADSMETLRETVTTQVRKLDGVKSTLTMIIVKDK"
     gene            12685..13689
                     /locus_tag="VLB45_00165"
     CDS             12685..13689
                     /locus_tag="VLB45_00165"
                     /inference="COORDINATES: protein motif:HMM:NF013832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04152.1"
                     /translation="MADTLFFMLGWLGELVFASWLLSYGAEHLAERWGAKFVGRTLLS
                     VATTLPEIAIVAYAAADGSYDAALGSALGSNLLMMTLGLAIMLIIATTRLSKAPQKLI
                     DVHQFKLDKILLMITAILGAVLFIDGYDFLDGIIFTGLFAVYLYFAFREIKHEKAAAS
                     LETETHEKKIHQSKKHFARAMIIFIAGTIGIFIGAEPFVHSLKDMSIESGVSVVVLAV
                     IIAPIAGEMPEKISMILLARKGAAGAAIAVANVLGSKILNNTLLLSVAVFGAMAYRGF
                     GEKIPNTPLLEYQMILVTVITLIALIPMFRNKLDLKSGIMLLSLYVIGIGLQFVLPAL
                     "
     gene            complement(13686..15041)
                     /locus_tag="VLB45_00170"
     CDS             complement(13686..15041)
                     /locus_tag="VLB45_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HSD04153.1"
                     /translation="MSASDAVLSKAKSLHIDECETFFADRKIITIRITDSEIVEIKQN
                     QERSLAIRTIHQKRIGSVKTSNPDQKDLVELALRSASLVKPKNYWKSLPSSAKFVPVQ
                     NTYDKKLEDISGVQASDIAQDMINAASNQKVTSISGSLNIVFEKIKIANSNGLNCTDK
                     ATYIAGTINTESNLGDSPVSGIGAVSSRTMDGFDAETVGREAADMCINSINSQKCEPE
                     IYSIIFEPYAIGEMLAFVFSSNFNLKIYSEKRSCFVDSLGKKIAKESFSLIDDPHYPD
                     GIGSKPFDDEGVPTMPNSFIENGIFANTFSDSFHAFKENKESSGNASRPGSPMGRSAQ
                     PIPFPAPHNLRIKGDGLSKDEMVRDTKKGLLVGRLWYTYAVNPERGDFSCTARSGIRI
                     IENGKIKSSGKSVRIVHNLPVLLQNISSIGNDVKKVLNWHSLPAITPSVKVEGIKVIP
                     I"
     gene            complement(15042..16307)
                     /gene="cofH"
                     /locus_tag="VLB45_00175"
     CDS             complement(15042..16307)
                     /gene="cofH"
                     /locus_tag="VLB45_00175"
                     /EC_number="2.5.1.147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010194456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(D-ribitylamino)uracil--L-tyrosine
                     4-hydroxyphenyl transferase CofH"
                     /protein_id="HSD04154.1"
                     /translation="MIIQASSFDSLLRQSDPLISETLDRALSEKEISAEEASRLFYAE
                     GAELHLIGMVADELRKRRVGNRVTYVINRNINFTNVCIKQCGFCAFSRDFREEEGYFL
                     PTDEIVRRAKEARSFGATEVCVQAGLPPDMEGDTYENICKAIKKEIPDIHIHGFSPEE
                     VLYGATRSNLSIKEYLIRLKESGVNSLPGTAAEILDQKMRDEISPGRISVKNWIEIIK
                     TAHKIGIPSTSTIMFGHVETPEDRTRHISLIREIQKETGGFTEFVPLGFIHTEAPMYK
                     HNLHSGIRSGPNGKDVLLMHAVARIMLNNVIDNIQISWVKEGRNMSQLLLAWGANDFG
                     GTLINESISTAAGAKHGQLLRPKEIRNFIRQLGRVPAQRTTSYKIIQEYDSEEKEDIL
                     DTVQDTSQFGSYQELIKLDKYRFKTNRRS"
     gene            complement(16304..17476)
                     /gene="cofG"
                     /locus_tag="VLB45_00180"
     CDS             complement(16304..17476)
                     /gene="cofG"
                     /locus_tag="VLB45_00180"
                     /EC_number="4.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7,8-didemethyl-8-hydroxy-5-deazariboflavin
                     synthase CofG"
                     /protein_id="HSD04155.1"
                     /translation="MLSKLISNYEALNKVIDGKELSKNDAYQIYQESQNSPSSLYATA
                     RVLRNKHKGKVVTYSRKVFFNLINLCRDTCSYCTYKSEPTQSKLSMMPKNDVKDLARL
                     GKKYRCTEALFVTGERPEEKYPQARKWLRENGFASTAEYLIHASGIALKEGLFPHTNA
                     GNLTKSEMNELKNTNVSLGLMLENSSDRLLEKGMPHQFAPSKDPKERIKVLENAGELG
                     IPMTTGVLIGIGETAFEIIDSLYAIKKIHEKFGHIQEIILQNFQPKPDTAMRRVPSAE
                     EAYFKNLVALARIIIPEMNIQIPPNLSPQSYSDFLSVGINDWGGISPVTPDYVNPEFS
                     WPSIDIIEKNSSDAGFELKARFPIYPEFVSFLHPDLKNSMSEISDSSGLVKMEYWK"
     gene            complement(17512..>17784)
                     /locus_tag="VLB45_00185"
     CDS             complement(17512..>17784)
                     /locus_tag="VLB45_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010194460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HSD04156.1"
                     /translation="IVSVATDSPAEKAGLHGASETKEIDGIRYQIGGDVILSVDGNDV
                     RKIDDILIYLQHEKQVGDTINLEILREGRITNFELVLQERPNLMQQ"
BASE COUNT         4715 a         3573 c         3138 g         6358 t
ORIGIN      
        1 ctcttctctc aacttctcct gtcacctctt ctctttttgg agcaatggaa tctatttcat
       61 cgatgaaaac aatagtaggt gacttttctc ttgcttcctt gaaaatttct cttaatcttg
      121 cttcgctttc accataaaac ttgctcatga tctcaggacc tgatatgctg ataaagtggg
      181 cgttgctttc gtttgcaaca gcttttgcca aaagcgtctt gccggttcct ggtactccaa
      241 aaagtaacac gccttttgga gcttcaactc caagcttttc aaaaatctct ggatgtctca
      301 ttggaagctc aatcatctct ctaacttttt ttatctctgt acccaggcct ccaatgtctt
      361 catatgatac ttgtggaaca ccatgaaggg aatcattttt ttctccgata tgaaattgtg
      421 tctctgaagt tacaatgacc gcatcagcca ctggcgtgat tcctattact tgaaaggtca
      481 gtcttccacc aaaatacggc accattatgt tatcgccttt taccaacggt gtattttcca
      541 gcgagtctgc aagatatttt tcatctattg gaggaattgt atcaagtgga gcaacaatga
      601 ttttttctgc aggaactgcc tttatctttc taacttcaat tttatcacct acggccactc
      661 ccaaattatt tcgtacaaga ccatcaactc ggataatttc ttttccttca tctgatggat
      721 aaagcggcat gcattttgca acagttcttc tttttccttt gatttcaatt atatcaccgg
      781 ttgctacgct aagtgaattc gtagaatcgt aatcaaggcg tgctacacct ctcccaacat
      841 cacgagaata tgcttccagt actttaagca aaataaaatt ttggcttatg tattatcccc
      901 accagtctaa cccttatagc tttgctattt tgccatgagg catatcgatc tgataaaatt
      961 gatcacggat tggtaaaaag attagatctt tagtgattct tttagtccct tgtacctgtt
     1021 tcgtattgta acttcggtta ctccagctgc cattgcaata actttctgtg ttacgttttc
     1081 tccgctcatc acacatgaaa gatataatgc agctgctgca agtcccatag gatcttttcc
     1141 agctgtaatt tccatcttgc ttgcatcagt taagattttg agagctcgac gttttacctt
     1201 ttcactcagt tcggccttgc ttgctattct agacatacat tttattggat caactactgg
     1261 catctttaga tccagttctc gtaaaagaag tctgtagcac cgtgaaacat cctttctttt
     1321 tatgtttatt tcacctgata cgtcgtttaa tgttcttggt gtttcactgt ctctacacgc
     1381 tgcatataat gcggctgcaa gaagacctgg aatggatctt cccctgacaa gacctttctc
     1441 aagtgccttt ctgtaaatgt aagctgtctt ttctattact gcatctgaaa gtgcaagctt
     1501 gtcttttaat cttccaagat cactaaatgc ttgtctaaag tttttgtctg aagattcatg
     1561 tacctgactt ctgctgtccc atgttctaag acgctctatg gtgcttttca ttgaagccga
     1621 caaaggtttt ccagttacat ccttgtttgc atggccaatt attgtggcaa gacccatgtc
     1681 atgcattgca agtgaggttg gtgaacctgt tctgcttctg tcatcatatt cgtcttttga
     1741 aaatgaacgc cattctggac ctgcttcctc gactctttca gtaataacaa aaccgcatcc
     1801 accgcaaaac ctctcgccag tagcattatc agttaacatt gaattttttc cacagcgtgc
     1861 gcacttggca ccagtattaa gagtttgtaa aaccatatta atctctgaca agtatcaggt
     1921 cgtcttctta tatgaactag agtattctga aattttgctc tcactggatg tatagatcca
     1981 tcattttaag gaaaaaggaa agaccttttc aggtcgaaaa tttttatctc ttggatttct
     2041 tactctttga ttttgctttt gatttggctt ttgccatgcg taatcgcact ttacattgct
     2101 attaagcctg ttgaaacgac tctattatcg ataaacgacg ttaatttcaa cttctaatgt
     2161 tcatttgtca tcaattctta ttgaggaaaa gctcatctta aaaccagaat ggaggtgtaa
     2221 tgatgagtta tagtggcggt tatggaagat ctaaccgagg atacggcggc ggcagagata
     2281 gaggatacgg cgacaataga cgatttaacg acggaccaaa accagttgaa accggaaaag
     2341 aatacgatgt ttccatagaa gaaattagta gaaaaggaga cggaatcgca agagtagatg
     2401 gctttgtgat atttgttaaa gacggaaaag caggtcaaaa agcaaaagta aagatcacac
     2461 aagttggtca acgatttgct tctgctgaaa tagtacaagg ttcttcacaa tccgaagcag
     2521 caccagcagc aactgaatag acaaaaatct gtctgattca aaattagttt ccaaaaattt
     2581 tttctttatt tttaatttca ttcatacaca agtgtgttgg cttgtgacca aatggtgtat
     2641 tgttaaataa aaattagaaa acacgatgca gtaatagaat aaaagtattg ttcataaatt
     2701 aatcgtacgc ttaattaata aattatagaa aaacagcatt ggcgaaggtt ccttttgctc
     2761 atgtggggag aggggaactc agacgcacca ccgtggtctg aacgatctca tgttaattgc
     2821 atgagcaaaa cccgccatta cattattaca attcttgcaa cagggtgttc acttttgaaa
     2881 taacttcgca tttttcaagt gcagtaagaa tcacagttct tagattttta atcctgtgat
     2941 gatcctctgg atataattct gatacattat ctatctcata attgaaacta taatctctaa
     3001 ggtatgcctt tgatgcatcc aaaaattcag aaaccatgtc tgaatcattg cctgactttt
     3061 ctatcttgtt gactagaact tgtagtatgc catgtatttt gccaagctcc tgctttattg
     3121 tggctaaatc gcctggcttg tctcttagct cagtgagtaa cttgtaagaa tttgaaatct
     3181 gtcccaaaag attcctgaca tattcatcat ccataccgtg tgggtgattc ataacctaaa
     3241 tgaattttct attgatcctt tttttctagt ttgagggata cggaatttat acaatatcgc
     3301 aagttggtcg gcttgggacc atcatcaaac acatgtccca agtgagctcc acatttgttg
     3361 cactctactt ctactcttaa catgccatag ctagtgtcag tttcgctttt tacatttttt
     3421 tcactgattg gagcaaaaaa gcttggccat ccagttccag aattaaactt ggtttctgaa
     3481 ctaaataatt cattaccaca acaaacacac ttgtacacgc ctttttcctt gcaattgtta
     3541 tactctcctg aaaaaggtcg ttctgtattt tttcttctgc atacttcgta ttgttcttct
     3601 gtgagatgtt ttttccactc ttcatccgat cttaacacat gatcatcttc tgacatgact
     3661 agaaaatgat cgaaaaagta ttagaatctt ctctaaacta ggtgtttgta gtttcgacta
     3721 ccttcaactt catcgttaat ggcgcggaca gtgcatcggg attatcgata ctttcccata
     3781 caaagatctg agcattgtat gttcctggtt ctgtaggagt ccatgactgt gatggattca
     3841 ttgattgtcc aggttcaagt attccagtta tccatgacaa tgaaattgtt atgccctctt
     3901 cattttgtat ctgtaccaaa tatgcaaatg gttgtgttct aatctgattg tttgttaaat
     3961 ctgcagcaat ctgtacttgc ttatttacct gaacttccca tgcttcgcct gaaagattta
     4021 cagaatatcc acctattgcg gcctgtgcat agtgagcaga ggttgataaa ataagaacaa
     4081 caagaaatgc agataacatc atcatggtgt tgttatcggg atctttgtcc atttgttttt
     4141 ttctatcgtt gttttcccac agtttagcaa tgctccgcat cataaagcca cctacaacta
     4201 ttattgcaaa accaccaatt aaaaaaacta ttacctttac aatatctttc agttttgcca
     4261 tgcttataga aagtctgtca cacagttaaa tctcatcatc tatccgatgt acaatttttg
     4321 taacttcatc gaccatttcc ctcatgctac tttctatctt ttcctttgaa aatgaagtag
     4381 agagttttag catcgttttt accttaaaat tagcatcaat tgtaaccttt gttccttctg
     4441 gaactgtctc gaaagtctca ataatatggg ttcctttgat atcgccagaa agtacaaaga
     4501 cttcatgtag ttcaaacggt tttatcacat gttttgttaa catcactaga tcccttcctg
     4561 caattcttac atgttcttcc accacgaaaa tgttgtccct gacagaacgt atctttagca
     4621 atttgaaata ctgtggcaag atgttaggaa aattttcaaa atccgttttc aggttgaata
     4681 ttttctctat ctggcaatta atgatcttgg tgaaactaat ttttgacaaa gaaaaatctc
     4741 acactacgtg tatgagatcc tttaattttc ttcttgatct tggtccacgt ttttgttcgg
     4801 ggtatcctat gcaaaggagc gatgaaggtc taagttttgt tcctactgct tccttgactt
     4861 gttcctcatt gatcattcca atccaaatgg agctcaagcc taatgctgac gcagcaagct
     4921 gtgaatatgc agctgcaagt gttgcatctt gaattgaaaa cttggcaaga acttcttgcg
     4981 ggaagtttag tttgacacgt tccggattca tacaaaatac aagtattatg ggtgtattga
     5041 catatggttg attgtttgcc gcctttacaa gcctctcttt tacttttggg tctttcacgt
     5101 aatagatctc aaacccttga aaattccctg ctgttggggc cgtgtctgca gctgcaataa
     5161 ttttgtctat tttccattgt tctatgggtc ttggagaaaa ttttctagtt gagcgtcttg
     5221 ttgacatgac ataaaacaga tctgtgttta cgtgcttttc tggtactttg gatgtaagga
     5281 taacattcaa aatattactt ctagaatctg tttcgggtat gactgcaggg gtatagcctt
     5341 ttggccagac tcttgtttct ttttgttcct ttggcatgtt ctgactagta aatgtgtaat
     5401 tataagatgt actaaaattg tctgctacgc agtaggatta ttttttacaa actcactaat
     5461 tttttcacca agtgttttag tttcaatttg tagaatctct ttgcctgcca tgaaaaattc
     5521 tgtggtgtca gagttcacca ttatggaact tacctcatct tcaagctcta tcttgtcggt
     5581 agaactgtga gacaagattt catgtaagtg aactagatcc tcgtgtctga gatagatcac
     5641 tctttcttca tcgtcatcat tacgccatat caatctgcat cctttttcat ttggtatgta
     5701 tggtgtatcg atctgaaaag aatggttcat cataccatgt caaaacaggc aaatttaaga
     5761 ttcttttggt ttgttttcta gctcgtttta cctcgaagag tttctaactc tccttttact
     5821 gcttcataat ctgtctttaa tctggaatga gccattttga ttatttcaag ttcttttttt
     5881 attgcgtcat attttgcatt tatggaagca acaacagaac ctgctgctac aacgactctt
     5941 tgcgtatcct ctttttgttt tactgtagat aattcatttt ctataaactt taattctctt
     6001 tgtcctactg tggctaattc cttttctatg aactctaatt ctttttttgc agtcattatt
     6061 tcatttttct ttttcatttg ttggttcagt aatgattgta attctgattt ggcgtattct
     6121 atttgagaat tgattcctga aagctctatt ctgactgttt ctattctctg tttaatgctg
     6181 tgtacttcat tttgatcata ttgtgatttt accaatgcaa gttcatattt tgctgtttca
     6241 agctctgata aggtagtttt gtaatctgat cttacctctg agagttggat tttttcttct
     6301 tctagttcag gttttatctt ttctaattca gcttttatct tttctagttc attagatttc
     6361 gcattgtatt cagattgtat gtattccagc ttgtgagacg ccgaatctac tttaactatt
     6421 gtttcttcta gctcattttt tgtatatttt aattcatttt ttgttgcttc gagttgagtc
     6481 tcttcaattt cttttacttt tatcttgtgc aaattttttg aatcagtgat cgttctgcca
     6541 tcctcacttt cctttgaacg tttaagcaat cccatttata tcacactcat atgctgtgaa
     6601 gaaaaccatt tctttcattc ttattatttg aattgctcat acttattcaa ttctttttat
     6661 gaccttaaaa attcaatctg ttttctttcg ttttgcaaat cctagataaa aaccaatacc
     6721 gccaactatg aagccaaata ctattgcaat ctgaccctcg attgaaccac gcggaagaaa
     6781 taatgttgca gaaatgaaaa gaaatgcccc aaaaagtatc attgcataac ttccattctt
     6841 ttttggacga ttgtgttctc tatgagccat atctagatgt attctgaaag ggattgtgca
     6901 actttttttc tacctatgtc cagtatgaat gggccaatct tagggccacg atcagatgct
     6961 aggattattt ggtataatgt tttgaaaaag tccttgggtt gtataccgcc agcttttgca
     7021 atattgtata ttgaattttg cagatctaca ggttcgtttt cagatgacaa catcgttatc
     7081 aattctttta tggcattttt ggttgcatcg tcaagctgaa tctcaacttt tgacggcttg
     7141 ccaaaatcat ctgcatagtt tccagccaga cttataagat gttcaatttc agcagttgat
     7201 tcctttatgg tgccatactc tatgagtttt tttacaacca gttcacttct ttgctctcgg
     7261 aatatcttgc aaagctcaac aagcagtcta taatttacat gtgcagttgt tgtctttgga
     7321 acatttaaca gatttacata ttcgtaaagg ccttttgctc ttactgtctt ggtttcgttt
     7381 tcaagtttta tttttccaaa gtacacattt tcaagttcat tgtattcatc cataagtgca
     7441 gggatgtcct caaaaccaac ttcgcgggtt ccggtaattc ttttgtatag taataacaaa
     7501 atagattttg aagttccata ctttagccat gcttgtgagg taacaacatt gccaagtgat
     7561 ttggaaattt tcttgcctcc tttatctaaa aacatttcat attttacgtg atgtggaggt
     7621 ggaaacttta gtatctcatc tgcaacccag tcatttacct taaccgaatc catgatatct
     7681 tttccatatg cctcaaatct cacatcaaat gcttgccatc ttgcagcaaa ctctactttc
     7741 catgcaagtt tgccaagatc ttttgtaata tctgctattc catcatgacc acaacctgct
     7801 aagggtttgg aaccaatagt ggtctcatgg catctgtact tgattttttt ttcatctgga
     7861 agatattcgt aagcttcagc tgtgtacagt cttccacagc tagaacatac aggaaaatat
     7921 ggaagtgcat cttggtattt ttcttggcca accatctctg caattttttc tccgattttc
     7981 ttactgttta gcaaaatggt atgaatctga tcttttagaa ggccgttttt gtatgtgtca
     8041 acacctcttt gaaatttgta tttgataccg catttgtcta atccgtcaag caaaagactg
     8101 ctcatgtgaa ggccatacga atcatgacat ccaaacggat cgggaataaa ggaaacaggt
     8161 tttgcaatgt gatcatttag ccagtctgga aatccctctg gaatctttcg tagaccgtca
     8221 aggtcgtctg agtatgcaat aagctcagac ttgtagccta ggttttctag tgcaagcttt
     8281 actccatatg cacgtgcagc atctccaagg cttccgatat gtggaatacc tgacgcgcca
     8341 aggccgcttt ctactttgat catggaggta ttgcgtccaa gagttttttc tctttctact
     8401 agctcgtatg caagcttgtc tatccatgtt cctctgccaa tggttttttg ttcttccatt
     8461 atgataattc ctttgacatg tatgaaccta aaggtgaata accgattttt ttgtaatact
     8521 ctcttgttcc aacagcactg ataacaagta gtctatttgc atcaaattcc tctcttgaaa
     8581 tcttttcagc ttcattcatg agatttttgc caagtcccaa atgttgtaca ctgtcagtgt
     8641 ctctttgtcc aagcttaagt gattttccgt atacatgtaa ttctcttaca atgcaagtat
     8701 tttgtgtgac ttctgatctg tgcgctagtc tacttggttt tcttaatctt aaaaatccaa
     8761 aaattctatc acttgaatca tcataagaaa gaaagacttc gtttccgcca gatgattcat
     8821 aatcttccct gtttagtttt atatcatcta cattgattga gctttcagaa agtcctgctt
     8881 ccctgcatct tatacatctg caagaaagat tttgtttttt gaggttgttt tgaactatct
     8941 ggcgcaggtt accaagtttt ggaccagcaa ttatttgatc agatgatatt tctctttgaa
     9001 ctcgcattat tcttacccac cgtggaatca tttttttgat ttcagtaaga acttcaacca
     9061 tatcttgatc tgaataaggc atgaacttgc catccctgta ggtttggtaa agtggtgtat
     9121 tttctaaaat gagtgttgga tagatcttga gcatgtctgg tttgaaggcg ggatcttcaa
     9181 agagtttttt gaaatcagaa atgtcttctt ttggagtcat tgtaggaagc ccaggcatca
     9241 tgtgagctac aatcttgtat cctgcatctt ttgatatctg aaatgattct attacatcat
     9301 ttaggtcatg tcctctgttt actatttcat atacacgttc atgtaagctc tgaactccta
     9361 tctcaacacg cgtaactcca tagtctagca tccagtctac gtgtgttttt ttgcaataat
     9421 ctggttttgt ctctatggta aaaccaacgt ttcttatttt tgcacgctca ttatttttct
     9481 tggcagattc caagtcttga gaatcaaacc cgttaattgc gtcatagcat gattttatga
     9541 aatttacctg atagtcttgt ggcataaaaa gaaaagttcc gccaactatt acaagctcaa
     9601 gcttggttcc atcatgtccg tatgcttgca ggtgtttgag cttgtctaat atctgctttt
     9661 ttggatcata ctcatttaca attgcatttt gagtagaagg ttccatcccg gtgtagctat
     9721 ttggtgtgtt aaattccact cctccaggac agtaagtaca tcttccatgc ggacatgcat
     9781 atggttttgg catcaaagca attactgcaa caccagaagc tgtcttgatg ggctttcgca
     9841 ataatgcctt ttgtaatttg agaaaatcat tcccatgagc ttctgcaagt atttcataat
     9901 tccttggtat tctttcaagt gaatatttca cacatgcttt tttgatttct tttttaacat
     9961 catttcttgt tggatttgaa atggcaagaa gattttgtga aatctctaca catgcttctt
    10021 caaacagtat ttttgatttt tgcactattt tttaaaaaga aattggctat taaatccttt
    10081 aagcaattct taatgaatcg acaaaaactc cttttttttc tgttatgatg ccgatttgcc
    10141 tgcttttcag accgtgtttt ttgaatatct catcaatctg ttctccttcc tcttctggtg
    10201 ctacaatgca gaatcctatg cccatgttga aggtcttgta catctctgat ttttccacat
    10261 tctggttttc aattaactgg aatatctctg gcggctctgg catgttatca agcatgtatc
    10321 ctgtcttttt gagacgtagg agcttggtaa aagaaccacc tgttatgtgg gcaagtccat
    10381 gtatgtcgca ttcctcaagt gcttcaagta caggtttcac atagatctcg gttggttcta
    10441 gcaatgcatc acccaatatt cccattcctc taattttgtc ttttatggaa tattttgaaa
    10501 gcagaacttt tcttgcaagt gaatatccgt ttgaatgcaa tccactgctt tttattccta
    10561 ttatgacgtc gcccggtgtt atcatgtcac caagaaccat ctgatttttt gtaagtatgc
    10621 caactaccat gcctgcaaga tcaaaagaaa actcctttcc agttatgaca tctggcatga
    10681 ttgcagtctc gcctccaact attggcactt gggatttttt tgcacctttt accaatcctt
    10741 gtacaatttt tttaaaaatt tcttggtcgt ttttatttgc agcaatgtaa tctacaaatg
    10801 atattggcgt agcaccagtg cagattatgt cgttaacatt catggcaaca cagtctatac
    10861 caatagtatc atattttttc atcatttgtg atatgataac tttggttcca actccatcag
    10921 tgtgtgttgc aacaagtttt cctccaccga tttctacaat tcctgcatag tgtccaaaac
    10981 ctgacgctat tctagctttt ttctgcagtt tgtgcgttga tgcaattagt ctgccaattg
    11041 ttgcttggct ttgttttatt tttttaatgt ctatgccagc atccttgtaa gtaactacca
    11101 atgatttgga atcctgttgt tttgaaataa aagaatttgt atgtactaca aattaaatat
    11161 aatgtataat atataaaaaa tactacatgt ataaaccaga atctgccttt cttgcctctg
    11221 cgtacttgtc tgaaagctcc ttaaagtcat gacctatttg ttgttcttgt tttcttagtt
    11281 ctgtaacatc tattgctgta ccataagcac ggtttaccgc atcaactagt gttgcagctg
    11341 caagagtgtc tggtctttgt ggatttgcct tgacaagtaa tgtgacacca cgaatttttc
    11401 tgatcagaga ctcgtttagt atggcaccag gaattccagt tacaaatcca cgtggaatca
    11461 tgtttatgcc gctatcttgc atgactctac aaagatcttc ttcagcagca caaaatgctt
    11521 tctgatcatg ttcttcttta tcggcaacac cgtccaaaac aaccagctcc ttagatccat
    11581 ttttttcggc ccaatcaaga attgaagatg cgatagtgta aagtccttcc attctcaaag
    11641 ttatttcgca aattattcca cagattgttc ctcccttgtt tgcatagaag cgaaatggat
    11701 gtcgaagcct tccctgcata aataccgtgg cgggaggcaa gtactttgat cgcatatatc
    11761 ctatctcttt catttttaat ttttcaatta tatatccaac tgcaatagag cctgcaagtc
    11821 ctgctcctac aaaacctgca aagattatgg ggctgtcaag ttttattttt ttgatctcaa
    11881 aaacttcggc ctctggaaaa ccattacgcg gcattacctt cgtgaatttg tcctgtctaa
    11941 ttacttttct gcatgaacag atctagcatg attctgccct tgtcagttat ggaatatatc
    12001 atgtttgacc cgactgccat ctttgtgata aaactgtact ttacacaaag atgcaggtag
    12061 ttgagaaacg attttttcat gcgtatgttt gacttcatat atagatcgga aaatgtcatt
    12121 ggactgcttt ttagttgata tagcagcttc aggagcgata ttgtgctaaa atcacgtgcc
    12181 cttgccttta cagagtccag tacctgctcg tctcttgtta ttataaaatc agagaaatgc
    12241 tctgccactt ccaaggggat gtaagtcatt cttgtcttca tgttaccgta atggtacggt
    12301 accttacctt attaatcttg acttggaaat ttgttgagct gaatgggatt ttttttcaac
    12361 aattacaatt tgtttttatg ccagtcaaag aattgtctgg tatgcatagc gcatttgtct
    12421 tgatgaatgc tgaacttggt aaggaaaaca aacttgtcaa ggaattaagg cagatcccca
    12481 acgttaaaga agtttatggt gtttatggtg tctatgacgt tttgatggaa atagaggcag
    12541 actcgatgga aactttgcgt gaaactgtaa caacccaagt aagaaaactt gacggcgtca
    12601 aatctacttt aactatgata attgtaaaag acaaatgatc tcaaaaataa tggcttataa
    12661 catacaataa aaacaaagta gcaattggca gacactttat ttttcatgct tggctggctt
    12721 ggcgagcttg tatttgcaag ctggcttttg tcatatggtg ctgaacatct ggctgaacga
    12781 tggggggcaa aatttgttgg aagaacactt cttagtgtgg caacaacgct gccagaaatt
    12841 gccattgtag catatgccgc ggcagacggt tcatatgacg ctgcacttgg ttctgccctt
    12901 ggtagcaatc tcttgatgat gactttgggt cttgccatca tgttgattat agctacaaca
    12961 agactgtcaa aggctccgca aaaactcatt gatgtacacc agttcaagct tgacaaaatc
    13021 ctgttgatga taaccgcaat tcttggggca gtattgttca ttgacggata tgactttctt
    13081 gacggtataa tctttacagg actatttgca gtgtatctgt actttgcatt ccgtgagata
    13141 aaacacgaaa aggcagcagc atcgcttgaa actgagacac atgagaaaaa gatacatcaa
    13201 tcaaaaaaac actttgcaag ggcaatgata atcttcatag ctggaacgat tgggattttt
    13261 attggcgcag agccatttgt ccattctctc aaagacatgt caatagagag cggagtttca
    13321 gttgttgtac ttgcagttat catagctcct atcgcaggag aaatgcctga aaaaatatcc
    13381 atgattcttt tggctcgcaa gggagcagct ggagcagcaa tcgcagttgc taacgtactt
    13441 ggttccaaga ttcttaacaa tacattgcta ctttctgttg cagtctttgg cgcaatggcc
    13501 tatagaggat ttggagaaaa aattccaaac acgccgctat tggaatatca gatgatactt
    13561 gtcactgtaa ttacgctgat tgcactaatt ccaatgttta gaaataaact tgatttgaaa
    13621 agtggaataa tgcttttgag cttgtatgta attggaatag gattgcagtt tgtcttgcca
    13681 gcgctttaaa ttggaattac cttaatccct tcaactttaa cagatggcgt aattgcagga
    13741 agtgaatgcc aatttaacac ctttttcaca tcatttccga tcgaagaaat gttttgcaac
    13801 aaaactggaa gattatgtac tatcctgaca gactttcctg aactttttat cttaccattt
    13861 tctattattc tgattccgct tcttgcagta caagaaaaat ccccacgttc tggattgaca
    13921 gcatacgtat accaaagtct gcctacaagc agtcctttct ttgtatctct taccatttca
    13981 tctttcgaca aaccatctcc ctttattctg agattgtgtg gtgctggaaa tggaattggc
    14041 tgtgcggatc tgcccattgg ggaacctggc ctgcttgcat taccagacga ttccttattt
    14101 tccttgaaag catgaaatga atccgagaag gtatttgcaa aaatgccgtt ctcaatgaaa
    14161 gagtttggca tggtgggtac accttcgtca tcgaatggct tgcttcctat cccatcagga
    14221 tagtgtggat cgtcaattag gctaaaactt tccttggcta tttttttgcc aagactatca
    14281 acaaaacagc ttcgcttttc tgaataaatc ttgagattaa agtttgatga aaacacaaag
    14341 gcaagcattt cgccaatcgc atagggttca aatattatgc tgtatatttc tggttcacat
    14401 ttttgggaat tgatagagtt tatgcacata tcagcagctt ctctaccgac agtttctgca
    14461 tcaaatccgt ccattgttct tgaagagact gcacctatgc cactgactgg agaatctcca
    14521 agattcgatt ctgtatttat tgtgcccgct atgtatgtag ccttgtccgt acaattcaaa
    14581 ccattgctgt ttgcaatttt gattttctca aaaacaatat tcaaggagcc tgatattgac
    14641 gtaactttct gattgcttgc tgcattgatc atgtcttgag caatatcgga agcctgaact
    14701 cctgaaatat cttctaattt tttatcatat gtgttttgta ctggaacaaa ttttgcactg
    14761 gaaggtaatg acttccaata attttttggt ttgacaagtg atgctgatct taaagccaat
    14821 tctaccaaat ccttctggtc tggatttgat gtctttacag agccaattct tttttgatgt
    14881 attgtcctta ttgcaagtga tctttcttgg ttttgcttta tttcgacaat ctctgaatct
    14941 gtaattctta tcgtgattat cttcctgtct gcaaaaaatg tctcgcactc gtctatgtgt
    15001 aatgatttgg ccttggaaag cactgcatcc gatgcagaca actaacttcg cctatttgtc
    15061 ttgaacctgt atttgtccag cttgattagt tcttgatacg agccaaactg tgacgtgtcc
    15121 tgcaccgtgt ctaagatgtc ttctttttct tcagaatcat actcttgtat tatcttgtat
    15181 gatgtagttc tctgggcagg aactcttcca agctgtctta tgaaatttcg tatctctttt
    15241 ggtctcaaaa gctggccgtg ttttgcccct gccgcagttg atatgctttc attgatcagt
    15301 gtgccaccaa aatcattagc gccccatgca agcaaaagct gtgacatgtt tcttccttct
    15361 tttacccatg agatctgaat gttgtctatg acattgttta gcattattct agcaactgca
    15421 tgcatcaaga gaacatcttt tccattagga ccacttctta ttccactgtg caggttgtgt
    15481 ttgtacatgg gtgcttccgt atgtatgaat cctaacggaa caaattctgt gaatccgcca
    15541 gtttcttttt gtatctcacg tatcaatgaa atatgtcttg tcctatcctc tggagtttct
    15601 acatgtccaa acattatggt tgatgttgat ggaattccaa tcttgtgagc tgtctttatt
    15661 atctcgatcc agtttttgac acttattctt cctggtgaaa tttcatcacg cattttttgg
    15721 tctaaaattt cggctgcagt tccaggaagc gagttgactc cagattcttt taatcttatt
    15781 agatactcct tgatcgacaa gtttgatctt gtcgctccat acaacacttc ttcaggagaa
    15841 aatccgtgga tgtgtatgtc tggaatttct ttttttatgg ccttgcaaat gttttcgtat
    15901 gtgtctcctt ccatgtctgg aggaaggcca gcttggacac atacttcagt tgcgccaaaa
    15961 gatctcgcct cttttgcgcg tctaactatc tcatctgttg gtagaaaata gccttcttct
    16021 tctcgaaagt ccctactgaa agcacaaaat ccgcattgtt tgatgcagac atttgtaaag
    16081 ttgatgtttc tgtttatgac ataagtaact ctattgccta ctcgcctctt tcttagctcg
    16141 tctgcaacca tgccgataag atgtaattct gcaccttctg cataaaataa tcttgaagcc
    16201 tcctctgcag aaatttcttt ttccgaaagt gctctatcca aagtctccga tataagagga
    16261 tctgactgtc ttagcagaga atcaaatgat gatgcttgta taatcatttc caatactcca
    16321 tttttaccaa tccactggaa tctgaaatct ctgacatgga gttcttaaga tctggatgca
    16381 aaaatgaaac aaattctggg tatatcggaa atcttgcctt aagttcaaat cctgcatctg
    16441 acgaattttt ttctattatg tctattgagg gccatgagaa ttctggattc acataatctg
    16501 gagtaactgg tgatattcca ccccagtcat ttatcccgac agaaagaaaa tctgaataag
    16561 actgtggaga aagatttgga ggaatctgga tgttcatttc tggaattatt attctggcaa
    16621 gtgcaactag attcttgaaa tatgcttctt ctgcagaagg aactcgtcgc attgcggtgt
    16681 ctggctttgg ctgaaaattt tgcaatatga tttcctgtat atgtccaaat ttctcatgaa
    16741 tttttttaat ggcataaagg gaatctataa tctcaaaagc agtctcaccg attccaatga
    16801 ggacccctgt tgtcattgga attccaagct cccctgcatt ttctaacact ttgatcctct
    16861 cctttggatc tttacttggt gcaaactgat gaggcattcc tttttccaaa agcctatcac
    16921 tagagttttc aagcatcaga ccgaggctga cattggtatt ttttagctca ttcatctcag
    16981 attttgttaa atttccggca tttgtatgcg gaaacaatcc ttcttttagt gcaatcccag
    17041 aagcatgtat caaatattcc gcagtacttg caaacccgtt ttctcttagc cattttctag
    17101 cttgaggata tttctcttct ggtctctcgc ctgtcacaaa tagtgcttcc gtacacctgt
    17161 atttttttcc aagtcttgca agatctttga catcattttt tggcatcatg gaaagctttg
    17221 actgtgtagg ttcagacttg tatgtgcagt atgagcaagt gtctctgcat agatttatca
    17281 gattgaaaaa tacttttctt gaataagtga caacttttcc cttgtgctta tttcttaaaa
    17341 ctctggcagt tgcgtaaagt gaagaaggac tattttgtga ttcttgataa atttggtaag
    17401 cgtcattttt tgataattct tttccatcta taaccttgtt taatgcctca taatttgaaa
    17461 tgagcttgct caacaccaca aatacctttg ttggcactat ttatgcttgt attattgttg
    17521 catcagattt ggtctttctt gaagaactaa ctcaaagttt gtgatgcgcc cttctcttag
    17581 tatttccaag ttgatagtat cgccaacttg cttttcatgc tgtaaataaa tcaagatatc
    17641 atcaatcttt cgtacatcgt taccatctac tgataaaatg acatctcctc cgatttgata
    17701 tcgtataccg tctatctctt ttgtttcaga ggctccatgt agtcctgctt tttcagctgg
    17761 gctgtctgtc gctacactta ctat
//
LOCUS       DATIDL010000003         4548 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrosopumilaceae archaeon isolate SMAG_U16342
            k141_2425256, whole genome shotgun sequence.
ACCESSION   DATIDL010000003 DATIDL010000000
VERSION     DATIDL010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935836
            Sequence Read Archive: SRR25158326
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosopumilaceae archaeon (soil metagenome)
  ORGANISM  Nitrosopumilaceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrosopumilales;
            Nitrosopumilaceae.
REFERENCE   1  (bases 1 to 4548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/04/2024 05:09:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,520
            CDSs (total)                 :: 1,474
            Genes (coding)               :: 1,474
            CDSs (with protein)          :: 1,474
            Genes (RNA)                  :: 46
            rRNAs                        :: 1 (5S)
            complete rRNAs               :: 1 (5S)
            tRNAs                        :: 43
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4548
                     /organism="Nitrosopumilaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2425256"
                     /isolate="SMAG_U16342"
                     /isolation_source="soil"
                     /db_xref="taxon:2202732"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..376)
                     /locus_tag="VLB45_00190"
     CDS             complement(<1..376)
                     /locus_tag="VLB45_00190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04157.1"
                     /translation="MADTFLGHVSGIFYDTANKKPEDVSFTLSDKINFSLLTHTTKPE
                     VTIPLPKILDQKYYFNGFVFENSEESRQSIWTLLIASIYHLASHVAISDYSIYGEWCK
                     NKSRDVSWRVIDFIEDAVVARYL"
     gene            complement(378..1391)
                     /locus_tag="VLB45_00195"
     CDS             complement(378..1391)
                     /locus_tag="VLB45_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016939375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HSD04158.1"
                     /translation="MTYISNVVNPSEKDVMLSKIKNADFSLNVKISKIKRNDSTLLKN
                     YNIEKYLDPSKFHFNTEMEQLIPKDTPPYLDNGERYVERISRALSYFKQCALIGPSGT
                     GKTHIVYLVAELAKLPLWEINCGLQTSVFDLFGRYIGLGKDNWIDGLIVSWCRYGGIL
                     YLDEANMMKQDIATRLNPILDTRGHMVLTEKDNEIVQRHKDAYLIISMNPFSAEFAGT
                     KPLNAALRRRMSVWLNFDYMSTGEKIDEKEVNLIMEKTNLDRLISENIVKVGAKLRNQ
                     YKIGDLPYAPSVGDLINWARLVADGSTVEEAGEETIIGVTSDDLEVQDFVRKIINSTF
                     RSK"
     gene            1490..2023
                     /locus_tag="VLB45_00200"
     CDS             1490..2023
                     /locus_tag="VLB45_00200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04159.1"
                     /translation="MNPEIRIDTISISKLGNNHDSFNKYDIEAALEEIENTEIETKVK
                     YAFTLLSNPKNTRINVEGVAHIRGNSSETSQFLEQDKNHLPRILYVIYQELFPLLYTN
                     SLAMQIPCPSYKLAQISSPAQTTETIPVEETVSNSTVDLPNESATPNDLPISNEIQTN
                     DVISEEIQKSEIEVQTS"
     gene            2080..2538
                     /locus_tag="VLB45_00205"
     CDS             2080..2538
                     /locus_tag="VLB45_00205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04160.1"
                     /translation="MRRQSRHSKRGLSTVVVSGILIAAVAIMGSAVVSWSNSNMMTRQ
                     QSLETTYVTNVNKIKEYLAIENIWFGGTTPANKFVNMTFSNAGSIGLNITETKLVTST
                     STAEFSYTNGGIFPSKINSTQRTYNWQDDVPIDVIVTTARGSIFRTQVTP"
     gene            2535..>4548
                     /locus_tag="VLB45_00210"
     CDS             2535..>4548
                     /locus_tag="VLB45_00210"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG-like jellyroll fold domain-containing
                     protein"
                     /protein_id="HSD04161.1"
                     /translation="MRSRKGLSTVVGAIFFVIAASSTIGYVTYSMNTVDRYNQSVLEK
                     NQEDVERSDEQFKITRVTVDNNKFNITIQNTGSLPVNITRLWVENTTDTDDIDRVFKY
                     DIKKAVYPGENVVKVGQTLPFYVKSSQAYDLKLVTERGNEQEFSVNSANAQPLQLQLF
                     ATPEKLPTGFTTTLLLAVTNNMSNNGILTNLVPILTPTDFGSTSALASGPEPTSVSVL
                     EQGNTAFFKWTYTVSGEPGEYTNFEARLTNGYPGNTVTESVYVDIVSESEQSQTSLKS
                     LGLGAQNSPDDILYFHLETTDALTGRQLKPVNTDSTGQTIQLDTTNAMFYTQNDTAPT
                     INIPSGGWNAYLRYLSAPLPDSLLNEGENMIFHFENGPSNPDDSTSDDLDLELCTGGK
                     QPTYEPTNGPNGTGTYHFTGAQCIRSLNNVENNKNDIGATPDTTALWFKLDTPLPTTK
                     QTLVRWEKNGGSPVKYYEISVPGTGSNAGKIVFEFNTQSTGSGEITTCVSNNAVKRVD
                     DGNWHHVVAVRKDGSGNPQQKCELYIDGILHSSVQSPHNTPNMEADGKWYVGSNRAEN
                     ADFFKGRIDDIIHWNSKALDATEALDLFKTSYGENAHKVTFKIDKTDKDGNLIQNLRT
                     NSSYPLDFLDGDGKYNSPSAADWKWFNFTTPTIDSVSLTNQRIRFNM"
BASE COUNT         1578 a          850 c          789 g         1331 t
ORIGIN      
        1 ataaataccg ggcaactaca gcgtcttcaa tgaaatcaat aacgcgccaa gatacatctc
       61 gtgatttatt tttgcaccat tctccgtata ttgaatagtc tgatattgct acatgtgatg
      121 ccagatgata gattgatgca atcaataacg tccagataga ctgacgtgat tcttctgaat
      181 tttcaaatac gaaaccatta aaataatact tctggtctag tatttttggc aatgggattg
      241 taacttccgg ttttgttgtg tgcgttagta atgagaagtt gatcttgtcc gatagagtaa
      301 aactaacatc ctctggtttt ttattagcgg tatcataaaa aataccagaa acatgaccaa
      361 gaaatgtatc tgccatttta tttggatcta aaagttgaat tgattatttt tctgacaaaa
      421 tcctgtactt ccaaatcatc actggtaaca cctattatgg tctcttcgcc tgcctcttct
      481 acagtagatc catctgcaac tagtcttgcc cagtttatca aatctcctac agaaggagca
      541 tatggtagat cgcctatttt atactggttt cgtaatttag ctcctacttt gacgatgttt
      601 tctgaaatta atctatcaag attagtcttt tccattatga gattgacttc tttttcgtca
      661 attttttcac ctgtactcat ataatcaaaa ttcaaccaca cactcattct tcttctgagt
      721 gcagcattaa gcggttttgt tccagcaaat tccgcagaaa aagggttcat actaattatg
      781 agatatgcat ctttgtgacg ttgtactatc tcattgtctt tttctgtcag taccatgtgt
      841 cccctcgtat ccaagattgg attgagtctt gtagcaatat cttgtttcat catgttggcc
      901 tcatcaagat ataaaattcc tccatatctg caccaagaaa ctatcagacc gtcaatccaa
      961 ttatcttttc caagaccaat gtatcttcca aacaaatcaa aaacagacgt ctgcaaacca
     1021 caatttattt cccataatgg aagttttgca agctcagcta ccagataaac aatatgtgtt
     1081 ttgccagttc cactgggtcc aatcaaggca cattgcttaa aatatgaaag cgctcttgaa
     1141 attctttcca catatctttc accattatca agatatggcg gagtatcttt tggaataagc
     1201 tgctccattt ctgtattaaa atgaaatttt gatggatcca gatatttttc aatattgtaa
     1261 ttttttaaaa gagtagaatc attacgtttt atttttgaaa ttttaacatt caaactaaaa
     1321 tccgcatttt tgatcttcga tagcattaca tccttttcac taggattaac tacgtttgat
     1381 atgtaagtca cgcagataat atctcaaaga atagttaatc ataaggggtg tattacagta
     1441 ctacatacag ggggttaaat cataactcat tcatcattaa aagacacaga tgaatccaga
     1501 gatacgaata gacacaatat ccattagcaa actaggcaat aatcatgatt cttttaacaa
     1561 atatgatata gaggctgcac tagaggaaat tgagaatact gaaattgaga cgaaagtcaa
     1621 atatgcattt acattactct caaacccaaa aaatactaga attaatgttg aaggtgtagc
     1681 acacatacga ggaaattcgt ctgaaacatc acagtttcta gaacaagata agaaccatct
     1741 tccacgtatt ttgtacgtga tatatcagga gctctttcca cttttgtata caaactctct
     1801 tgcaatgcaa atcccatgtc cctcatacaa gcttgctcag atttcatcac ctgcacaaac
     1861 tacggaaaca ataccagttg aagaaacagt ttcaaattca acagttgatt tgccaaatga
     1921 gtcagcaaca ccaaatgatt tgccaatatc aaatgagata cagactaacg atgtcatctc
     1981 agaagaaatt cagaaatccg aaatagaagt acagacatcg taagtcatac cagattacat
     2041 acacatcgat aatacttgat ttaatgcagt tttttggata tgagacgtca gtctcgacat
     2101 tcaaaacgtg gtcttagtac tgtagtagtt agtggaatac taattgcggc agtcgcaata
     2161 atgggcagtg ccgttgtttc ttggtcaaat tctaacatga tgactagaca acaatcatta
     2221 gaaacaacat atgttacaaa cgtcaataaa atcaaggaat accttgcaat agaaaacata
     2281 tggtttggcg gtacaacacc tgctaataaa tttgtcaaca tgacattcag caatgctgga
     2341 agtataggtc ttaatattac ggaaacaaaa cttgttactt ctacaagtac tgcagaattt
     2401 tcatatacaa atggaggaat atttccaagc aaaataaact cgacgcaaag gacatacaat
     2461 tggcaggacg atgtgcctat agatgtaatt gtaaccactg caagaggctc tatttttaga
     2521 actcaggtga caccatgaga agcagaaaag gtcttagtac tgtagtagga gcgatttttt
     2581 ttgtcatagc cgcatcatct acaattggtt atgttacgta cagcatgaac acagttgata
     2641 gatacaacca atcagttttg gagaaaaatc aagaagatgt agaacgtagt gatgaacagt
     2701 ttaagattac aagagtaact gtggataata ataaattcaa tattaccata cagaatacag
     2761 gcagtctgcc tgtcaacata acacgacttt gggtggagaa tactacagat acagatgata
     2821 tagatcgggt tttcaaatac gatataaaga aagcagtata tcccggtgaa aatgttgtca
     2881 aagtaggcca aacacttcca ttttatgtta aatcatcaca agcatatgat ctgaaattag
     2941 taactgaacg gggaaatgaa caagagtttt cagtgaattc tgcaaacgca caaccactac
     3001 aactacaatt gtttgctact cctgagaaac ttccaactgg gtttacaact acgttgcttt
     3061 tagcagtaac aaataacatg tccaataatg gaatattgac aaatcttgta ccaatactta
     3121 caccaactga ttttggatca acatctgcct tagcgtctgg tccagagcca acatcagttt
     3181 cagttttaga gcagggaaat acagctttct ttaaatggac atacacagta tcaggagaac
     3241 ctggagaata tactaatttt gaagcaagac tcactaatgg ttatccagga aatacagtta
     3301 cagaaagtgt ttacgttgac attgtatcag aatcagaaca aagtcaaact tcattaaaat
     3361 ctcttgggct tggagcacaa aactcacccg atgatatatt atattttcat ctagaaacta
     3421 cagatgcatt aactggacga cagttaaaac cagttaatac ggattctact gggcaaacaa
     3481 tacaacttga taccactaat gcaatgttct atactcaaaa tgatacggca ccaacaataa
     3541 acataccatc cggtgggtgg aatgcatatc tacgatatct tagtgctcct ctacctgata
     3601 gcttactcaa tgagggtgaa aacatgatat ttcattttga aaacggtcca tcaaatcctg
     3661 acgattcaac ttctgacgat ttagatttag agttatgtac tggtggtaaa caaccaacat
     3721 atgagccaac aaatggtcca aacggtactg gcacatatca ttttacaggc gcacaatgta
     3781 tacgaagtct taacaatgta gagaataaca agaatgacat aggagctact cctgatacta
     3841 ctgcattatg gtttaaactt gatactccat tacccacaac aaaacagacg ttggttcgct
     3901 gggaaaaaaa tggtggcagt ccagtaaaat attatgagat atctgttcca ggaacaggtt
     3961 caaatgcagg taagatagta tttgaattca atacacagag taccgggtcc ggagaaataa
     4021 caacttgtgt tagtaacaat gcagtcaaaa gagttgatga tggaaactgg catcatgtag
     4081 tagcagtaag aaaagatggt tcaggcaacc cacaacaaaa atgtgaattg tatattgatg
     4141 gcatactcca tagttctgtt cagagcccac ataatacacc taacatggaa gcagatggaa
     4201 agtggtatgt aggaagtaac agagctgaga atgctgactt ttttaaaggc agaatagatg
     4261 acataattca ctggaactca aaagcattag atgccacaga agctttggat ttgtttaaaa
     4321 caagctatgg tgaaaatgca cataaagtca cgtttaaaat cgataagact gataaagatg
     4381 gaaaccttat ccaaaattta agaaccaaca gcagctatcc attagacttt ctagatggtg
     4441 atggtaagta caacagcccc tcagctgcag attggaaatg gtttaacttc acaacaccaa
     4501 ccattgatag tgtatcctta acaaatcaga gaatacgatt caacatga
//
LOCUS       DATIDL010000004         3121 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrosopumilaceae archaeon isolate SMAG_U16342
            k141_1621918, whole genome shotgun sequence.
ACCESSION   DATIDL010000004 DATIDL010000000
VERSION     DATIDL010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935836
            Sequence Read Archive: SRR25158326
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosopumilaceae archaeon (soil metagenome)
  ORGANISM  Nitrosopumilaceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrosopumilales;
            Nitrosopumilaceae.
REFERENCE   1  (bases 1 to 3121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/04/2024 05:09:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,520
            CDSs (total)                 :: 1,474
            Genes (coding)               :: 1,474
            CDSs (with protein)          :: 1,474
            Genes (RNA)                  :: 46
            rRNAs                        :: 1 (5S)
            complete rRNAs               :: 1 (5S)
            tRNAs                        :: 43
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3121
                     /organism="Nitrosopumilaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1621918"
                     /isolate="SMAG_U16342"
                     /isolation_source="soil"
                     /db_xref="taxon:2202732"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1598
                     /gene="acs"
                     /locus_tag="VLB45_00215"
     CDS             <1..1598
                     /gene="acs"
                     /locus_tag="VLB45_00215"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007550249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HSD04162.1"
                     /translation="KSLGVAKGDRVTIYLPMVPELIIAMNACARIGATHTVVFSGFSA
                     KSLRDRIEDSKSKVILTADGGFRRGTVVKLKQVIDEAIQGFDFVQHVVVLERTKTPVT
                     LNKKDKKWSELMQNAQDFCEPEKLDSAHPLYILYTSGTTGKPKGVLHGTGGYLTHLYS
                     TFKWIFDIKDSDVYFCTADIGWVTGHSYVAYGPLLHGATQVMYEGAPDYPTPARMWEL
                     IQRYKITIFYTTPTALRMFMKFGDKIPNSHDLSSLRLLGTVGEPINPEVWKWYFETIG
                     KEKCPIVDTWWQTETGGAIITPMPGLETIPLKPGSATRPIPGIDVAVVDESGKELPVE
                     TKGYLVIRTPWPGMLLTLWNDDEKYKSVYWSKYKNIYYPGDYAIKDKDGYFWLLGRAD
                     DVLKIAGHRLGTAELESGFVSHKSVAEAAVCGIPHDVKGEAIIAFLVLREGSTPNDLL
                     KNEIINHIRTVIGPIATPDQLYFVSKLPKTRSGKIMRRLLKSIAKGEAIGDVSTLENE
                     AAVSEVQAALEELKLSLQQNKIK"
     gene            complement(1591..2085)
                     /locus_tag="VLB45_00220"
     CDS             complement(1591..2085)
                     /locus_tag="VLB45_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007401866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HSD04163.1"
                     /translation="MASRAQVLIPVIIAAAIVGIVGIITIPSDHKLQSVDFPKGTIKV
                     DNIVLDVEVADTDSTRARGLMFKEQLSYNQGMIFVFEEEQIASMWMLNMQFPLDVIWF
                     DEDGNVVHIEKNAEPCKSALETMACTFKNGNDEKSKYVLEVTAGFVDKFNITEKSKLE
                     IISI"
     gene            2207..2665
                     /locus_tag="VLB45_00225"
     CDS             2207..2665
                     /locus_tag="VLB45_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008299297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron dependent repressor, metal binding and
                     dimerization domain protein"
                     /protein_id="HSD04164.1"
                     /translation="MKEKHGERLESIKQAHKQGKGNSHTRIEDYLEVISELVELKGYA
                     NTIDISRYMNVSAPSVTKMLRRLDKNGYLEYEKYHGINLTAKGNSIADAIRQKHGILV
                     EFFKILRIDHQIANQDAEGIEHHLNPKTIKQLRKFITYLKSNPKILESFQ"
     gene            complement(2666..>3121)
                     /locus_tag="VLB45_00230"
     CDS             complement(2666..>3121)
                     /locus_tag="VLB45_00230"
                     /inference="COORDINATES: protein motif:HMM:NF012243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HSD04165.1"
                     /translation="AKSGGIEKLAESKIRETIRRFDPDAEIEILSNNSIQVRVDKEVM
                     PSIIGRGGSTINELEKALGVHIDVEPKVHTFGNEIDFELSEAGNNIVLEVDETKVGMY
                     ADIYVKGQYLLSAHVGKKGRIKISKRSESGKKIINAVMAKQDIKLILHN"
BASE COUNT         1002 a          604 c          617 g          898 t
ORIGIN      
        1 taaagtctct tggagttgca aaaggagata gggtaacaat ctatcttcca atggttcctg
       61 aattgattat agcgatgaac gcatgtgcaa gaatcggtgc aacacatact gttgtctttt
      121 ctgggtttag cgcaaaatca ctaagggaca gaatcgagga ttcgaaatcc aaagtaatac
      181 taactgcaga tggtggattc agacgcggta ctgttgtaaa attaaagcaa gtcatagatg
      241 aagccataca aggttttgat tttgtacaac atgttgttgt tttggagcga acaaaaactc
      301 ctgtcactct aaataaaaaa gacaaaaaat ggagtgaatt gatgcagaac gcacaagatt
      361 tttgcgagcc agaaaaattg gacagcgcgc acccattgta cattctttat acgtctggca
      421 ctacaggaaa gccaaaaggc gtgttgcatg gaactggcgg ttatcttacg catctgtatt
      481 ccacattcaa atggattttt gacatcaagg attctgatgt ttatttttgt actgcagaca
      541 ttggctgggt cacaggacac agctatgtag catacgggcc attacttcac ggtgcaaccc
      601 aagtaatgta tgaaggtgct cctgattatc ctacaccggc aagaatgtgg gaattgatac
      661 aacgatacaa aataacaatt ttttacacta ccccaactgc actgcgcatg ttcatgaagt
      721 ttggtgacaa gattccaaat tcacacgatc tttccagttt gcgtctgctt ggaactgttg
      781 gcgagccaat aaatccagaa gtttggaaat ggtattttga aaccatagga aaagaaaagt
      841 gtccaatagt ggacacttgg tggcagacag aaaccggcgg tgcaataatt actcccatgc
      901 caggactaga aacaattcca ctaaaaccag gttctgcaac aagaccaatt cctggtatcg
      961 atgtagctgt tgttgatgaa tcaggaaaag aactacctgt agagacaaag ggatatctag
     1021 ttatcagaac tccttggcct ggaatgcttt tgacactatg gaatgatgat gaaaaataca
     1081 aatcagtcta ttggtcaaaa tacaaaaaca tctactatcc tggagactat gcaataaagg
     1141 acaaggatgg gtacttttgg ttacttggac gagctgacga tgtcctaaag attgcaggtc
     1201 acaggctggg cactgccgag cttgaaagcg gatttgtctc ccacaaatca gttgccgaag
     1261 ctgcagtctg tggaattcca catgatgtaa aaggcgaggc aatcattgca tttctagttt
     1321 taagagaagg ctccacacca aatgatcttc tgaaaaatga gattatcaat cacataagaa
     1381 cagtgatagg tcctattgca acacctgatc agctgtactt tgtttcaaaa cttccaaaga
     1441 ctagaagtgg taaaatcatg agaagactgc taaaatctat agccaagggc gaagctattg
     1501 gagatgtaag tacgctagaa aatgaggcgg cagtaagtga ggtacaggca gctcttgagg
     1561 agctaaaact atcactacaa caaaacaaga tcaaatagag attatctcaa gtttggactt
     1621 ttctgttatg ttgaatttat caacaaatcc tgccgtgact tctaaaacat attttgattt
     1681 ttcgtcattt ccattcttaa atgtacacgc catggtttca agcgcagatt tgcatggctc
     1741 tgcatttttt tcaatatgta caacattgcc gtcttcatca aaccatatta catctaaagg
     1801 aaattgcatg tttaacatcc acatggaagc tatttgttct tcttcaaaca caaagatcat
     1861 gccctgattg tatgaaagtt gttctttgaa catcaatcct ctagctcttg tggagtcggt
     1921 atcagctact tcgacatcta gtactatgtt atctactttg attgtgcctt ttggaaaatc
     1981 aactgattgg agcttgtgat cgcttggtat tgttattatt ccgactatgc ctactattgc
     2041 agctgcaata attacaggaa tcaatacttg agctctggat gccacaaaat agaaagaaaa
     2101 agttctagtt aaaaactaac ggggatatag acaagttttg cagttccgta ttcacttgca
     2161 caaagaaatg agttatatca ttcgcggtat atcacgacag aagactgtga aggaaaaaca
     2221 cggagagcgc ctagaatcaa taaagcaagc ccacaaacaa ggaaagggca attctcacac
     2281 taggatagaa gattatcttg aggtcatatc tgaacttgta gagctaaaag gatatgcaaa
     2341 tacgattgac atatcgagat acatgaacgt aagtgctcca agtgttacaa agatgcttcg
     2401 acgactggac aagaatggtt atttggaata tgaaaaatat cacggaataa atctgactgc
     2461 aaaaggaaac tcgattgcag atgccatacg acaaaagcat ggaatcttgg tagagttttt
     2521 caagatactt agaatagatc atcaaatcgc aaaccaagat gcggagggaa tagagcacca
     2581 ccttaaccca aaaacaataa agcaactaag aaaattcatc acgtatttga aatctaaccc
     2641 aaaaattctt gaaagctttc agtagctagt tgtgcaaaat taatttaatg tcttgttttg
     2701 ccatcacagc gttgattatc ttttttccag attctgatct ttttgaaatc tttattctcc
     2761 ctttttttcc aacatgtgca gataacaagt attgcccttt cacataaatg tctgcataca
     2821 tgcctacttt tgtttcatcg acttcaagca ctatattatt tccagcttca gaaagctcaa
     2881 aatcaatctc gtttccaaat gtgtggacct ttggctccac atcgatatga acgccaagtg
     2941 ctttttcaag ctcgtttatt gtagagccac cacgaccgat gatcgatggc attacttcct
     3001 tgtctactcg tacctggatg ctattgtttg ataaaatctc aatttctgca tctggatcaa
     3061 acctacgtat tgtttctctt atcttggatt ctgcaagctt ttctattcca ccgctttttg
     3121 c
//
LOCUS       DATIDL010000005         9267 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrosopumilaceae archaeon isolate SMAG_U16342
            k141_2026735, whole genome shotgun sequence.
ACCESSION   DATIDL010000005 DATIDL010000000
VERSION     DATIDL010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935836
            Sequence Read Archive: SRR25158326
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosopumilaceae archaeon (soil metagenome)
  ORGANISM  Nitrosopumilaceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrosopumilales;
            Nitrosopumilaceae.
REFERENCE   1  (bases 1 to 9267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/04/2024 05:09:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,520
            CDSs (total)                 :: 1,474
            Genes (coding)               :: 1,474
            CDSs (with protein)          :: 1,474
            Genes (RNA)                  :: 46
            rRNAs                        :: 1 (5S)
            complete rRNAs               :: 1 (5S)
            tRNAs                        :: 43
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9267
                     /organism="Nitrosopumilaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2026735"
                     /isolate="SMAG_U16342"
                     /isolation_source="soil"
                     /db_xref="taxon:2202732"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..532
                     /locus_tag="VLB45_00235"
     CDS             <1..532
                     /locus_tag="VLB45_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HSD04166.1"
                     /translation="ESANDELAHLKDEMRLIRNAVQKEIPVLGICLGSQLIAKAFGAM
                     VYQGKKKEIGIYHDVEFDNASKSELFAGIKSPATVFHWHGDTFNLPQGAIRLAHSANY
                     ENQAFQIGTAVGVQFHLEVDEATIRLWLEKSKEELSQLPYIEPDKIIKQIPEHIQTIQ
                     NNMKIFYRNFKAAYNL"
     gene            574..2703
                     /locus_tag="VLB45_00240"
     CDS             574..2703
                     /locus_tag="VLB45_00240"
                     /EC_number="6.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010193324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxypropionate--CoA ligase"
                     /protein_id="HSD04167.1"
                     /translation="MSSITKVFEETIATDHKVITEELSKSVLKKYGVKVPGYALVNSA
                     KEATKAAKRLGYPLVMKVVSPQILHKTDVGGVKVGLQNEKEVKKAFNDMYKRLSKKKG
                     VHLKGILLEKMVPQGVELIVGLQNDPQFGPVIMVGLGGVLTEIFKDVAFRMLPITTAD
                     AKSMLEELKGAKILQGYRGSKPIDQNMLAKALVQISKLGVDNAAYFDSIDFNPIVVYP
                     KSYYVVDAKIILRKEIKPNAISKAQPNAEFMEKFFTPQSVALVGASATPGKIGNSVLD
                     SLAKHDYKGKVYPINPKAEEILGLKCYPSLEAIQEPIDLVVVCVDLSVTAPVLESCAK
                     KGIHDVVIVSGGGKELGGERAAMEAEVKRLSEMHKIRIIGPNCIGMFNAANRLDCAFQ
                     GQARMVRAKLGDVAFLSQSGTMGISFLETADSFGLSKMVSYGNRSDVDEADMIWYLAS
                     DPQTKVIALYVEGFGDGRKFIETAKRVMAEKHKPVVIWKSGRTAAGAKQAASHTGSLG
                     GSNAIIMGAFKQAGIISVDSYQELTGVVKVLSWQPAAKGNRVGLVSNGAGPMIGAIDH
                     FERLGLQVAKVSESTLAEMKAHYPPTYVLGTGNPVDVTGGANADDYRYTIQKFMDDPN
                     VDIIMPWFVFQDDPLEETIIDYLGEFSRQKKKPILVGGNGGPYTAKVSALIENHKVPV
                     YDDIRTWVAAAAGVLQWGRHNPPGQGS"
     gene            2716..3477
                     /locus_tag="VLB45_00245"
     CDS             2716..3477
                     /locus_tag="VLB45_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008297871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HSD04168.1"
                     /translation="MTLVNVSKSDGIATVKINRPDKLNAMNTDVAKELISVFNDIDKD
                     DAVKVIILTGEGDKAFSAGADIEYMSKISPIESETYAKTGHLLTSTVENVSKPTIAAV
                     NGFALGGGCELALACDIRIAADTAKIGQPEVTIGVCPGWGGTQRLQRIVGIAKAKELI
                     YTGRMVKADEAKSIGLVNQVVELPKLMEETMNMAKMIAQNSAMAVRMSKIAINKGRNS
                     DIDTGLALELYTWGLCFTHPDRSDRMTAFVNKSKK"
     gene            3560..3910
                     /gene="erpA"
                     /locus_tag="VLB45_00250"
     CDS             3560..3910
                     /gene="erpA"
                     /locus_tag="VLB45_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013481404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HSD04169.1"
                     /translation="MSNTQSTKLLTVTPKAAEKIAAFMKEEADKPEFLRVYVQGGGCS
                     GLSYGMSFEKKAEEDDVLIEENGVKVLVDSYSQDYLKGANVDYIESLMGAGFKINNPN
                     VTKSCSCGHSFNTS"
     gene            complement(3954..4202)
                     /locus_tag="VLB45_00255"
     CDS             complement(3954..4202)
                     /locus_tag="VLB45_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HSD04170.1"
                     /translation="MAQAYVIIHCETGMEDWVIRNLKKIGGVKEAVGVFSLYDIIVKV
                     EAKDAATLEKIITKQIRKVKHIHTTMTLMVIDEQESKR"
     gene            complement(4245..5492)
                     /locus_tag="VLB45_00260"
     CDS             complement(4245..5492)
                     /locus_tag="VLB45_00260"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008296737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HSD04171.1"
                     /translation="MVKIDPWQNALKQLDDAVKILKIDSGVRDILATPKKILTVALPV
                     KMDNGKIKVFTGYRSQHNDFRGPHKGGIRYHPDVTIEEVKALSMWMTWKCAIVDVPFG
                     GGKGGIICDPKKMSAGEKERMTRRYAYAISEIIGPGKDVPAPDVYTTGTEMAQIMDVY
                     SVMHGQTEPGVITGKPLSVGGSLARNVATGLGVAYCVREGAKKIGLKLKGAKVVIQGF
                     GNAGTFSGEYLEKMGAKVIAVSDSKGSIMNPKGLDVKKVLEYKNKKGTVVGYPGSKKI
                     TTEQLLTTPCDVLVPAALENQITPDIAKKIKCKIIAEAANGPTTPAADEILFKKKIMV
                     IPDILANSGGVCISYLEWVQNLQRYYWTFDEVAGKMEEHIVRGFNDTYNLAKKHKVDM
                     RKGSMVLAVSRVLDSFNARGLWP"
     gene            complement(5552..5899)
                     /locus_tag="VLB45_00265"
     CDS             complement(5552..5899)
                     /locus_tag="VLB45_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010193303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HSD04172.1"
                     /translation="MHDFSLNILGSEWVIIAFVAIVLFLGTKRLPELSKKIGKAVGEY
                     NKAKNMVESEIKNATAGYNINVQGPVQTERQKLETIAKSLGVDFLNKSDDELRNIISS
                     KMGQSQNPSETKA"
     gene            complement(5924..7081)
                     /locus_tag="VLB45_00270"
     CDS             complement(5924..7081)
                     /locus_tag="VLB45_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSD04173.1"
                     /translation="MNKPLVLVYATALIVGISYGMHNPIVPVFSKEVIGASYFDLGLI
                     GLSNFIPYMFIPLFVGLLLDRFNNGLLLTLGITIDALSIYLLSISQSVPEVAIFRAMT
                     GIAHSFFWPPCESIISRVVPPQARVKSISKFMGFFVGGLMIGPLIGTFLLEHFDVTYR
                     ILFQYATFAIATGIVSSLLLKKYGKTTHQTAFSFVSVKKILKFPTIVMMLIFCSSAFG
                     TFLTILPAYMNDRSISESHIELLFFVFGISRLVSLGFSELLARRTFHSLIAAMLSIAI
                     GMAIVAYSFSLESFAIAVLIMGFGFTVYFPLTFEIIMRKAQKENSGGLIGAYETTFGV
                     GWAAGPLFAGIVANFFGNATPYLMFFAIGIAVTTIVALKKKELAPAWNPKL"
     gene            complement(7083..7772)
                     /locus_tag="VLB45_00275"
     CDS             complement(7083..7772)
                     /locus_tag="VLB45_00275"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HSD04174.1"
                     /translation="MGLGSYWGDVLDVLRDIIPVYDKVNSFISLGKDEEFRTKGLSGR
                     VKEGNAVLDAGSGFGNMSKTASKLCGNNLNITLYDPLTPMLKNAKKFFENTPTLASGV
                     FEHIPFRDEEFDAVICGYSLRDAISLKTAISEIHRVLKKGGRFVIVDLGKPDHPFIRM
                     GVSFYLRMILPVIAFAAAGKLGLKFATLYGTYKLWPQNKKLEAMLLEKFSKVEFDTAM
                     MGGAIMVAAYK"
     gene            complement(7807..8700)
                     /locus_tag="VLB45_00280"
     CDS             complement(7807..8700)
                     /locus_tag="VLB45_00280"
                     /inference="COORDINATES: protein motif:HMM:NF013255.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HSD04175.1"
                     /translation="MLKAFVTGATGFIGSKLVAKLVDRGVSVTCLVRSGVVQNPAVKT
                     VNGDLTYPDFVLPDEKYDVVYHLAAAWPGEKDKKKARTVNYDGSVNLFSLAKDKTKSF
                     IYVSGLGVFGDPGNRIVDENSSLQPNTEYAKTRLEAQKFLESSCMEKSIDFSVAYLGD
                     VYGNGGWFRSIMVDRLKNGSFKLPGSGDYYRSFIHVDDAVSSLVAIAEKNTANQSFVV
                     TDSNPVLFKDYVNFVCDELGVKHPGSVPAFLAKAVMGGDFVKLLTTSVKTSNAKIAKL
                     TEFVYPSYKEGVHSVISEIKS"
     gene            8830..>9267
                     /locus_tag="VLB45_00285"
     CDS             8830..>9267
                     /locus_tag="VLB45_00285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HSD04176.1"
                     /translation="MQKTFNQKIMFGCSSCGLEDIVEYKKNVDEAYLDFLNKFDEGKI
                     LTKKKMQTALEEEGIVQAENEIKKMIGDAKLDELTNSILFSRKHFVSYFRTISEPEPK
                     MGNKIAELGLDESIVQVLEEKGIERFYKFQEESLLEIMRGNNVI"
BASE COUNT         3015 a         1812 c         1811 g         2629 t
ORIGIN      
        1 tgaaagcgca aatgatgagc tggcacatct taaagacgag atgcgattga tcagaaatgc
       61 cgtacaaaaa gagattccag tacttgggat ctgccttgga tctcagctta ttgcaaaggc
      121 atttggtgcc atggtctacc aaggaaagaa aaaggaaata ggaatatacc atgatgttga
      181 gtttgacaat gcatcaaagt cagagctttt tgcaggaatc aagagtccag ctactgtatt
      241 tcattggcat ggagatacgt tcaatctacc tcaaggtgca ataagactag cccactctgc
      301 aaattatgaa aaccaagcat tccagatagg aacagccgtt ggggtgcagt ttcatctaga
      361 agtggatgaa gcaactatca gactatggct agaaaaatca aaagaagaac taagtcagtt
      421 gccatacata gaaccagaca agataataaa acagattcca gagcacatac aaaccataca
      481 gaacaacatg aaaatttttt accggaactt caaggcagca tacaatcttt gacaaaagtt
      541 taatataatg acggctagac ttttcgatct ggcatgagtt ctataaccaa agtctttgaa
      601 gaaacgattg ctaccgatca caaagttatc accgaagagc tttcaaaatc tgttctgaaa
      661 aaatatggcg taaaggttcc aggatacgca cttgtaaatt ctgccaaaga agctacaaag
      721 gctgcaaaaa gacttggcta tccacttgtc atgaaagttg tatcaccaca aatacttcac
      781 aagactgatg ttggcggagt aaaagttgga ctgcaaaatg aaaaagaagt aaagaaagct
      841 ttcaatgaca tgtacaagag gctttcaaaa aagaaaggag tccatctaaa gggaattttg
      901 cttgaaaaga tggttccgca aggagtagag ctcattgtag gtctacaaaa cgacccgcag
      961 tttggtccag taatcatggt aggccttggt ggtgtcttga ctgaaatttt caaagatgtt
     1021 gcattcagaa tgcttccaat aacaactgca gatgcaaaat ccatgctaga ggagctcaag
     1081 ggtgcaaaga tactccaagg atacagagga agcaagccaa ttgatcaaaa catgcttgca
     1141 aaggcacttg ttcagataag caagcttgga gtagacaatg cagcctattt tgacagcatc
     1201 gattttaacc caattgttgt ttatccaaaa tcatactatg tagttgacgc aaaaataatt
     1261 ctaagaaaag aaatcaaacc aaacgccata tcaaaagcac agccaaatgc agaattcatg
     1321 gaaaaattct tcactccaca atctgtagca ctggttgggg catcagctac acctggaaag
     1381 attggaaatt ctgttttaga tagtttagca aaacatgact acaaaggcaa agtgtatccg
     1441 ataaatccaa aagctgaaga aattttggga ctcaaatgtt atccatcatt agaagcaatc
     1501 caagaaccaa ttgatttggt tgttgtttgt gttgatcttt cagttactgc accagttctt
     1561 gaatcatgcg caaaaaaagg aatccatgac gttgtcattg tatcaggtgg aggaaaggag
     1621 cttggtggag aaagagcagc aatggaagct gaagtaaagc gtctttcaga gatgcataag
     1681 atcagaatta tcggtccaaa ctgtataggc atgtttaatg cggcaaacag acttgactgt
     1741 gccttccaag gacaggcaag aatggttcgt gccaaattag gtgatgttgc attcttgtca
     1801 caaagcggaa caatgggaat cagcttcctt gagacagcag actcgtttgg attatcaaag
     1861 atggtaagtt atggaaacag atcagatgta gatgaggcag acatgatttg gtatcttgca
     1921 agtgatcctc aaaccaaagt aattgcactt tatgtagaag gttttggaga tggccgcaag
     1981 tttattgaaa ctgcaaagcg tgtcatggca gaaaaacaca agccagttgt gatatggaag
     2041 agtggaagaa ctgcagctgg cgcaaaacaa gctgcatcac acacgggatc acttggagga
     2101 tcaaatgcaa taatcatggg agcattcaag caagcaggaa taatatcagt tgacagttat
     2161 caggaattaa ccggagtagt aaaggtactt tcatggcaac cagctgcaaa aggaaacaga
     2221 gttggtctag tcagcaacgg tgcaggaccg atgattggcg caattgatca ctttgaaaga
     2281 ctgggactac aggttgccaa agtttctgaa tcaacacttg cagaaatgaa agctcactat
     2341 ccaccaacat atgtacttgg aactggaaat ccagtagatg tgactggcgg tgcaaatgca
     2401 gacgactata gatatacaat tcaaaagttt atggatgatc ccaatgtgga tatcataatg
     2461 ccgtggtttg tcttccaaga cgatccttta gaagagacaa tcatagatta tcttggagaa
     2521 ttctcaagac aaaagaaaaa accaattctt gttggaggaa acggcggacc atacacagcc
     2581 aaagtatcag cactgataga aaatcacaag gttccagtct atgacgatat cagaacttgg
     2641 gttgcagctg cagcaggtgt gctccagtgg ggaagacaca atccacctgg gcaaggatca
     2701 tagagtgaat ttaccatgac tctagttaac gtatcaaaat ctgatggaat tgcaacagta
     2761 aagatcaaca ggccagacaa gcttaatgca atgaatactg atgtcgcaaa ggaattgatt
     2821 tctgttttta atgatataga caaagatgat gcggtcaaag taataatact aacaggagaa
     2881 ggagacaagg cattttctgc aggagctgac attgaataca tgtcaaagat ttctccaata
     2941 gaatcagaga cttatgcaaa gacagggcac cttcttactt ctactgtaga aaacgtcagc
     3001 aaaccaacca ttgcggcagt aaatggattt gcacttggag gcggatgtga acttgcactg
     3061 gcatgtgaca ttagaattgc tgcagacact gcaaaaattg gtcagcccga agttacaata
     3121 ggagtatgcc ctggatgggg tggaacacaa agactgcaac gaatagtagg aattgcaaag
     3181 gcaaaagaat tgatctatac aggaagaatg gtaaaagctg atgaagcaaa atcaattgga
     3241 ttagtaaatc aggttgtcga attgccaaaa ctcatggaag aaacaatgaa catggcaaaa
     3301 atgattgcac aaaattctgc aatggcagta cgaatgtcaa agattgcaat aaacaagggg
     3361 cgaaactctg acatcgacac aggtcttgca ctggaacttt acacatgggg attgtgcttt
     3421 acacatccag acagatcaga cagaatgact gcatttgtca ataaatcaaa gaaatagacg
     3481 ctcaattact tctcagcggg tattccgaat atgtcatgta ggaaaacctt attataattt
     3541 aaaagacgaa taaaatcaaa tgagcaatac acagtctaca aaactactca ctgtcactcc
     3601 aaaggcagct gaaaagatag ctgctttcat gaaagaagag gcagacaagc cagaattcct
     3661 tagagtatat gttcaaggtg gcggatgctc cggtctttct tacggcatga gctttgaaaa
     3721 gaaagctgaa gaagacgatg tcttaattga ggaaaacgga gtgaaagtct tagtggacag
     3781 ctatagtcaa gattacctaa aaggtgcaaa cgttgactat atcgaaagtc taatgggcgc
     3841 tggatttaaa atcaacaatc caaacgtaac taagagctgc tcttgcggac attctttcaa
     3901 tacttcctag gttctgacaa aattttttat ttttatctta tctagcgcat gctttagcgt
     3961 tttgattcct gctcatctat aaccatcaga gtcatggtgg tatggatgtg ttttactttt
     4021 cgtatttgtt ttgttattat tttttcaaga gtagctgcat cctttgcttc aacctttaca
     4081 atgatatcat aaagactgaa tacaccaaca gcctccttta cgcctccaat ttttttcaag
     4141 tttcgaatta cccaatcttc cattcctgtt tcgcagtgaa tgatcacata agcttgggcc
     4201 atgattatac ttgcactcgc tgttagattt agttttgtga aatactaagg ccacaagcct
     4261 ctagcattaa atgaatccaa tacacgactt actgccaaga ccatgctgcc tttgcgcata
     4321 tccactttgt gtttctttgc caagttgtag gtgtcattga atccacgtac aatgtgctct
     4381 tccatttttc ctgcaacttc atcaaatgtc cagtagtatc tttgcaggtt ttgtacccac
     4441 tctagatatg atatacacac accgccagag tttgccagta tatctggaat taccattatt
     4501 ttcttcttga atagaatttc gtctgcggct ggagttgttg gtccgtttgc agcttctgca
     4561 attattttac attttatttt ctttgcaata tctggtgtga tttggttctc tagtgcagcg
     4621 ggaactagta catcacatgg agttgtaagc aattgttctg ttgtgatctt tttgcttcct
     4681 ggataaccaa caactgttcc tttcttgttt ttatattcaa gaactttctt aacatctagt
     4741 ccttttggat tcattataga accctttgaa tcgcttactg caataacctt cgctcccatc
     4801 ttttctagat attcaccgct aaatgttccc gcatttccaa atccctgaat aactactttg
     4861 gcacctttta gtttcaaacc aattttttta gctccttctc ttacacaata tgcgacacca
     4921 agtccagttg caacatttct tgcaagagaa cctccaacag aaagtggttt gcctgtaata
     4981 acgcccggtt cagtttgtcc atgcataaca ctatagacgt ccattatctg cgccatctct
     5041 gttccagttg tgtacacatc tggagcagga acgtcctttc ctggacctat gatttcagaa
     5101 atcgcataag catatcttct tgtcattctt tctttttcac cagctgacat tttctttgga
     5161 tcacaaatga ttccaccttt tccacctcca aaaggaacat caactatagc acatttccat
     5221 gtcatccaca ttgaaagtgc ttttacttct tcaattgtta catctggatg ataacggatt
     5281 ccacctttgt gtggtcctct aaaatcatta tgttgtgatc tgtatcctgt gaaaactttg
     5341 atctttccgt tatccatctt aactggaagg gcaactgtca atattttttt tggagttgca
     5401 agtatgtctc gcacaccaga atcaatcttg agaattttta cagcatcatc aagttgtttt
     5461 agtgcattct gccaaggatc aatctttacc aaattgtacc gatctgagtc actgtatatt
     5521 ctatttaaat ttgttgagga aaacagaaca attacgcttt ggtttcagaa gggttttgac
     5581 tttgccccat ctttgacgat attatgtttc ttagttcgtc atcacttttg tttaagaaat
     5641 caacacccag agatttagca atggtttcaa gcttttgtct ttctgtctgg actggaccct
     5701 gtacgtttat gttgtaacct gcagttgcgt ttttgatttc actttcgacc atgttttttg
     5761 ccttgttgta ctcccctact gccttgccaa tcttttttga tagttcagga aggcgttttg
     5821 ttcccaaaaa caatactatt gccacaaaag caatgatcac ccattcgcta cccagtatgt
     5881 taagcgagaa atcatgcatg attgaatttt acctattcca aaattaaagt ttaggattcc
     5941 atgctggcgc taattccttt ttctttaaag ctacgatggt tgtaactgca attccaattg
     6001 caaagaacat gaggtaaggc gtggcattcc caaagaaatt ggctacaatg cctgcaaaaa
     6061 gcggcccagc ggcccatcca actccaaatg ttgtttcata tgcaccaatt aatcctccag
     6121 aattttcttt ttgcgctttt ctcattatga tttcaaatgt tagtgggaaa taaacagtaa
     6181 aaccaaatcc catgatcaat actgctattg caaaagattc caaagaaaat gaatatgcca
     6241 caatagccat ccctatggca attgaaagca ttgcagcaat aagactgtga aaagtccgtc
     6301 tagccaacaa ttctgaaaat ccaagtgaaa ccagtctgga aataccaaaa acaaaaaaca
     6361 acagctctat gtgtgattca gaaattgatc tgtcgttcat gtatgctggc agaatggtaa
     6421 gaaatgttcc aaaggcagag ctgcaaaaga tcagcatcat cacaattgtt ggaaatttca
     6481 aaattttctt gactgataca aacgagaatg ctgtctgatg cgtggtcttg ccatattttt
     6541 tcaaaagcaa ggaagaaaca attcctgtag caatagcaaa ggtagcatat tggaataata
     6601 ttctgtacgt gacatcaaag tgttccagca gaaatgttcc aatcaatggg cctatcatga
     6661 gcccaccaac aaaaaatccc atgaacttgg aaattgattt tacacgagcc tgcggaggca
     6721 caactcgtga tatgatagat tcgcatggag gccagaaaaa cgagtgtgca atgccagtca
     6781 ttgccctaaa gatggcaact tcaggaacag actgcgatat agaaaggagg taaattgaga
     6841 gtgcgtctat tgttattcca agggtgagaa gaagcccgtt gttgaatcta tcaaggagga
     6901 gtccgacaaa aagcggaata aacatgtatg gaatgaaatt tgacaggcca atcaggccca
     6961 agtcaaaata cgatgcaccg attacttcct ttgaaaagac tggaactatt gggttgtgca
     7021 ttccatagga tatgccgaca attagtgcag tggcatatac caaaaccaga ggcttgttca
     7081 tgtcacttgt atgcagcgac cattattgcg ccacccatca tcgcagtatc aaactctact
     7141 tttgaaaact tttcaagcag catggcttca agttttttgt tttgtggcca cagtttgtag
     7201 gtcccataaa gagttgcaaa ttttaatcca agctttcctg ccgctgcaaa tgcaattaca
     7261 ggaagtatca ttctaagata aaatgaaaca cccatcctga taaatggatg atcaggcttt
     7321 ccaagatcaa ctataacaaa tcttcctcct ttttttaaca ctcgatgaat ttctgatatt
     7381 gcagttttaa gactgattgc atcacgcaac gaataaccac aaatcactgc atcaaactct
     7441 tcatctcgaa atggaatgtg ttcaaagact ccagatgcaa gagtaggtgt gttttcaaaa
     7501 aacttttttg catttttcaa catgggagta agaggatcgt aaagtgtaat gtttagatta
     7561 ttaccacaaa gttttgatgc agtctttgac atgttgccaa atccagaacc tgcatcaagt
     7621 actgcgttgc cttcctttac cctacctgac agcccttttg tgcgaaattc ctcatctttt
     7681 ccaagtgaga taaatgaatt taccttgtca tatacaggaa taatatccct taaaacatca
     7741 agaacgtctc cccaataact tccaagaccc atacgaaaat tagctttctt ggatattata
     7801 tgtcaatcag gattttattt cagatataac agagtgtaca ccttctttgt atgaaggata
     7861 tacaaattcc gtgagctttg caatctttgc atttgatgtc tttacagaag ttgtgagtag
     7921 cttgacaaaa tctccgccca tgactgcctt tgccaaaaat gccggaacgc tgcctggatg
     7981 ttttactcca agctcatcac aaacaaaatt cacataatct ttgaaaagaa cagggtttga
     8041 atcagtcacc acaaatgact ggtttgcagt atttttttct gcaatggcaa caagtgaaga
     8101 cactgcatca tcaacatgaa taaagctacg ataataatct ccagaaccag gcaacttgaa
     8161 acttccattc tttagtctgt caaccattat ggacctaaac catccaccgt ttccgtaaac
     8221 atcgccaaga tatgcaacac tgaaatcaat ggatttttcc atgcaacttg attccagaaa
     8281 cttttgtgct tctagtcttg tctttgcata ttctgtattt ggttgaagtg aagaattttc
     8341 atcaacgatt ctatttccag gatcaccaaa aacacctaga cctgacacat agatgaaaga
     8401 ctttgtctta tcttttgcaa ggctgaataa atttacagag ccgtcgtaat tgacggttct
     8461 tgcttttttc ttatcctttt cacctggcca agctgctgca agatggtaca caacatcata
     8521 cttctcatca ggcaaaacaa agtcagggta tgtaagatcc ccgtttacag tctttacggc
     8581 tggattttga acaactccgg aacgaactag gcatgtaacc gatactcccc tatcaacaag
     8641 ttttgcaaca agcttagaac caatgaaacc ggtggcgccg gtaacaaacg ctttcaacat
     8701 atcaaaaaca tattcgttgc ttaaaggttt aacctgagat caaaacggaa gatagtcaaa
     8761 aattaaattc aacacaaaac atgccatgat gtattgtatt cttgtcccaa atgcaaatca
     8821 aaggtggaaa tgcaaaaaac attcaaccag aaaattatgt ttgggtgttc tagttgtggt
     8881 ttggaagaca tagtagaata caagaagaat gtagatgaag catatcttga ctttttgaat
     8941 aaatttgatg aaggaaaaat tctgaccaag aaaaaaatgc aaactgcatt agaagaggaa
     9001 ggaatagtgc aggccgaaaa tgagataaaa aagatgatag gagatgccaa acttgatgaa
     9061 ctgacaaact ctattttgtt ttcaaggaaa cattttgttt cttattttag aacaatatca
     9121 gaaccagaac ccaagatggg caacaagata gcagaattag gattagatga aagcattgtt
     9181 caagttttag aagagaaagg cattgaacgg ttttacaagt tccaagaaga atcgttatta
     9241 gaaataatgc gtggaaacaa cgtaatc
//
LOCUS       DATIDL010000006        14298 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrosopumilaceae archaeon isolate SMAG_U16342
            k141_1826585, whole genome shotgun sequence.
ACCESSION   DATIDL010000006 DATIDL010000000
VERSION     DATIDL010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935836
            Sequence Read Archive: SRR25158326
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosopumilaceae archaeon (soil metagenome)
  ORGANISM  Nitrosopumilaceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrosopumilales;
            Nitrosopumilaceae.
REFERENCE   1  (bases 1 to 14298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14298)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/04/2024 05:09:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,520
            CDSs (total)                 :: 1,474
            Genes (coding)               :: 1,474
            CDSs (with protein)          :: 1,474
            Genes (RNA)                  :: 46
            rRNAs                        :: 1 (5S)
            complete rRNAs               :: 1 (5S)
            tRNAs                        :: 43
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14298
                     /organism="Nitrosopumilaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1826585"
                     /isolate="SMAG_U16342"
                     /isolation_source="soil"
                     /db_xref="taxon:2202732"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(49..585)
                     /locus_tag="VLB45_00290"
     CDS             complement(49..585)
                     /locus_tag="VLB45_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04177.1"
                     /translation="MTQDSLTASLKYYGVSPWEIEVLYGLLHDMFVVEQDPSAESEED
                     YSTVIRIDFPLEFSDEFFKWFGHVRWDKIKGILKEMKRRRGGGKNIKTYIHFSGKPNI
                     KFIADLDDHHWFSIAIEKIDFILELLQYQLDPQKIPPDVTDVVYEFDQSVGRWYLHTI
                     FSHNKKYVFSKSEWRLVT"
     gene            complement(723..893)
                     /locus_tag="VLB45_00295"
     CDS             complement(723..893)
                     /locus_tag="VLB45_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007401996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04178.1"
                     /translation="MSRTVSTRIPIQLHAELRERCNKIGCSISDYVAEAIKFAVYGNS
                     DFDFEESEEEAN"
     gene            complement(1118..1339)
                     /locus_tag="VLB45_00300"
     CDS             complement(1118..1339)
                     /locus_tag="VLB45_00300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04179.1"
                     /translation="MKYKTFESKEADHIITGKNTDDLIQPLFVVAKSVENLRKTFGSQ
                     IDKKAENMLSDLEKSIVDMLDTLDKLRMP"
     gene            complement(1583..1927)
                     /locus_tag="VLB45_00305"
     CDS             complement(1583..1927)
                     /locus_tag="VLB45_00305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04180.1"
                     /translation="MTFSGLKVSNKVLSDSQRQEFLVFLRMMSKKGFYEILLYVKDKE
                     NVGYNQVLKYAIDKKIVDSRASITIILNGLTYLGLLERTVTSDRPVRTNYEVTKTGNH
                     VIKAFKDLEHIL"
     gene            2180..2428
                     /locus_tag="VLB45_00310"
     CDS             2180..2428
                     /locus_tag="VLB45_00310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04181.1"
                     /translation="MAIEQALLDAGGSRLLKEVSNIMFEEFGCSIPDSLYNPDYLNKV
                     LKKLFGNSYTAILQSIRKNLDEFSYQEPIAEFLQAVGA"
     gene            2432..3247
                     /locus_tag="VLB45_00315"
     CDS             2432..3247
                     /locus_tag="VLB45_00315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04182.1"
                     /translation="MQTRLETLRKQKFLFIALIITSSLVVLANQFGEKMSMVVTDLIY
                     GPIIIILTVLAIISASTSDLKSDHGKAWLFFVLGAISWAIAEHLWMIYELVLEEDPFP
                     SAADFFYLGGYPFVFLFMIYYLKFIRTAISKKIIVAASLVALAIGAPSIYLSYDLDSE
                     ISTFDLILATTYPIVDAIIFVPAMIGVILFFSGRVNFMWTLMCIGIISLTLADAGFLA
                     AEYDGSYYTGHPIDILFIWTYVLFSFGIYDHIKIFREKVVHKTPMTERNPTET"
     gene            complement(3362..3916)
                     /locus_tag="VLB45_00320"
     CDS             complement(3362..3916)
                     /locus_tag="VLB45_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010193185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04183.1"
                     /translation="MVEFLTRYPKTISFFDGVKGKVGVDSKTGVEQLTIVVRKNVDEL
                     LRIFSKEGFTHVKFEHKQPFQIGNGLSLKLKKPWEMHVRLLDMKKGLIAIHAEVEVSR
                     DYLQHLFSQRTPVVYEIEALLKKHQIEYKIWNEKIKKYVNAIFDNYKVQLSTPKIPVF
                     AWKPMVFVIGTVGVLYLWKYLQTI"
     gene            complement(3958..4728)
                     /locus_tag="VLB45_00325"
     CDS             complement(3958..4728)
                     /locus_tag="VLB45_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HSD04184.1"
                     /translation="MSISIHPVKSELKMQEFDLFETLVDSLARSHIRLENGDVLVVSS
                     KYVANSQGRIIKYENVIPSVESQKLATKFHLNPKIAEIILRESDAVFGGVPGFVITSA
                     DNILAPNAGIDKSNAQRGSLILYPTDAYAVAEQLKRKFFLKFSIHIGIIIIDSRLMPA
                     RIGTTGVAIACAGIEPLSDIRGQKDLYGNPLKVTFQAVADNLASIANLKMGEGSDSTP
                     FAVIKNSRVAITDRKISQDEMAISHDQCVYVRGLKTNV"
     gene            4804..6081
                     /locus_tag="VLB45_00330"
     CDS             4804..6081
                     /locus_tag="VLB45_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008301374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HSD04185.1"
                     /translation="MGMNITEKILARASGNSNLSPDDVVFVNVDKVMVHDVSGPGVIA
                     VFEKLKKKGMKIEKLWDSSKVWVAEDHFVPSADKISAQNVVLLSEFTKTYGIEKHFKY
                     GMGQYGICHTLSHEEAMVLPGEVYVGGDSHTNTTGALGAFAAGLGHTDVAYVLLNGKI
                     WFKVPETLYFKLNGKLPSHVMAKDVILKIIGDIGTDGAAYKTMQFGGNGISEMSVEER
                     LTLTNMTTEAGAKSGIIEPDQKIRDYLAMRGATKYNEVHGDPDAQYEKIYEYEASEME
                     PIVAKPYSPENISPARDLSTIQLDKAYIGSCTGAKMEDLYAVAKILKGRKVKIRTEIL
                     PAAISIFRKAMDEGLLKIFMDAGVVVGPPTCGACCGAHMGVLAKDEVCISTTNRNFPG
                     RMGHIDSKTYLASPLVAAASAVTGKITDPRDLK"
     gene            6078..6560
                     /gene="leuD"
                     /locus_tag="VLB45_00335"
     CDS             6078..6560
                     /gene="leuD"
                     /locus_tag="VLB45_00335"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214804.1"
                     /note="catalyzes the isomerization between
                     2-isopropylmalate and 3-isopropylmalate in leucine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HSD04186.1"
                     /translation="MKGNAIKYEKDNIDTDVIIPGPYLKIHDYVELAKHAMEGLDKDF
                     HSKVKEGDFIVAGRNFGCGSSREHAPIALAYSGVKAVLALSFARIFYRNAVDGAFLLP
                     IEIEEDAYKNISDRDSIEIDTAKNEIKNLTKNQIYKMKPFSELIGKIIAAGGLFNYKP
                     "
     gene            6633..8048
                     /gene="leuC"
                     /locus_tag="VLB45_00340"
     CDS             6633..8048
                     /gene="leuC"
                     /locus_tag="VLB45_00340"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HSD04187.1"
                     /translation="MKKTLFEKIWDSHVVVEQNGGRSLLYVDRHLVHEVTSPQAFDGL
                     RMNKRKVRRPDLTFATMDHNVPTSDRSLPIIDQISAKQIQVLETNCKDFGIQLFDIHS
                     PNQGIVHVIGPELGITLPGTTIVCGDSHTSTHGAFGALAFGIGTSDVEHVLATQCIWM
                     EKPKTFEIKVEGKRKNPHAVTAKDIILSIIRQIGTSGGNGTVIEYRGEAISDLTMEQR
                     MTICNMSIEAGARAGLVAPDEKTFHYLRGRRYVPKNYEEYVDYWRENLKTDEGASFDK
                     SFVLHAEKIVPQVSWGTNPAMTVDVTGTVPFPEEYAKGNPEEEKAAVKALQYMDLKPG
                     VQITEIHVDRVFIGSCTNSRLEDLIEASRVIKGRKVSPKVRAMIVPGSQLVKKQAEDL
                     GLDKIFKEANFEWRESGCSMCLGMNPDILAAGERCASTSNRNFEGRQGTGGRTHLVSP
                     VMAAATAIAGHFVDVREMDLN"
     gene            8053..8628
                     /gene="leuD"
                     /locus_tag="VLB45_00345"
     CDS             8053..8628
                     /gene="leuD"
                     /locus_tag="VLB45_00345"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HSD04188.1"
                     /translation="MEPFKTVKSITTPLDKVNVDTDQIVPKQFLKLVQKTGFGKYLFY
                     DWRFTNDGKPKEDFVLNNPAYKNSRILLARENFGCGSSREHAAWALLDYGFRVVIAPS
                     FADIFYNNCFKNGILPITLNNKTMDTLFRFQNEIEVNLEDQTIKVGDSKISFEIENHR
                     KKTLLEGLDDIAVTLQYEDKVSEYEKTRDKF"
     gene            complement(8625..9353)
                     /locus_tag="VLB45_00350"
     CDS             complement(8625..9353)
                     /locus_tag="VLB45_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HSD04189.1"
                     /translation="MKAVILAGGSGTRGKPFTDYFPKAMIPIEGKPLIDYIVRYLSSI
                     DFIKEIIILADYNGLGSQIKSYFENRKDLKKIKFVQDSNSGTGGDLVHLLSHIKDGEF
                     TLWFVDNLCAINLKMMYKFHKKTKSIACIATRRYRSEETGHAIVKEGRILEFNEKPTI
                     QLEMPECLGIYILKTNILKMIRAKKTRKKNINLSYDVLQELSKKHTVSAFDIEKTPWV
                     DIESPIKVERNDKLIKNIIRKMKN"
     gene            complement(9357..9800)
                     /gene="rpsI"
                     /locus_tag="VLB45_00355"
     CDS             complement(9357..9800)
                     /gene="rpsI"
                     /locus_tag="VLB45_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HSD04190.1"
                     /translation="MVKIENYFASRKTARAHVHISKGDGRVRINNVPVEMIQQEIARE
                     VILAPLEIAGDLREKVDLSVRVNGGGFMGQAYASAVAISRAMTGWTKTKKEPKDHPFT
                     KTIREDLKKRLNEFDKHLLSGDDRRKEPKKFGGPGARRRKQKSYR"
     gene            complement(9800..10273)
                     /locus_tag="VLB45_00360"
     CDS             complement(9800..10273)
                     /locus_tag="VLB45_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008301322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HSD04191.1"
                     /translation="MAKQQAETKTMAKGNQTMIIDGTNQIAGRLCSRVAKLLLEGNRV
                     HIVNSENIMISGNREAIIKSYREFLEIASINNPRFGPFHPRRPDTIITKMVRGMLPKT
                     KSSGQAAFKRLRVYLGVPNELRSLERTQFEDAKITKPSPYYTSVGELGRMVGWHE"
     gene            complement(10266..10613)
                     /locus_tag="VLB45_00365"
     CDS             complement(10266..10613)
                     /locus_tag="VLB45_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008301403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18e"
                     /protein_id="HSD04192.1"
                     /translation="MTNQIVLQMAKEMKSASKKNKAPIWSRLAELALKPSSARRTINI
                     SQIDRLTKDNDTIVVPGKVLGTGNMSHKITLCSFSISNSGVQKIIQTGGKIMSYTELI
                     KTHPTGKGVIIIG"
     gene            complement(10657..11289)
                     /locus_tag="VLB45_00370"
     CDS             complement(10657..11289)
                     /locus_tag="VLB45_00370"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010192366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit D"
                     /protein_id="HSD04193.1"
                     /translation="MASLEIVEQNEHKIVVKLKGVPLQYANALRRICLSGVPVFAVDD
                     ITIIENSSVLADEGIAHRLAMIPLKTDLTRFVEPANCDCKSEVGCSHCRVMLMVDSGS
                     ADTTRTITTAEVTSEDDFVKPVNDKIPIVELAPGQKIKFEAYARLGRGTEHAKWNSAT
                     VSVLTETGNSEDRLLTVETTGALSPAQIVLTGVDELGKRLEGFKQVIAAL"
     gene            11375..12064
                     /locus_tag="VLB45_00375"
     CDS             11375..12064
                     /locus_tag="VLB45_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010192375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly factor SBDS"
                     /protein_id="HSD04194.1"
                     /translation="MTDKFTIIRFSVEGEKFELLVKPDPALEYKLGKRKDVSTVLVSD
                     EIYSDSNKGTRASTEKLMKAFKTTDQTTIATIILQKGDLNLTTDQRRKMVSEKRKQIV
                     DFIAKTYVDPRSHLPHPPLRIEQAMDDARVSVDPFKNIDEQTKEIVEQLRSIIPLKSE
                     NMLLEIHVPAQFAAQSYSVLKSAGTLKKEEWQSNGSLKVILDIPAAARANVIERLGSV
                     TKGSASVEVAK"
     gene            12064..12768
                     /gene="rrp4"
                     /locus_tag="VLB45_00380"
     CDS             12064..12768
                     /gene="rrp4"
                     /locus_tag="VLB45_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000178 - exosome (RNase complex)
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exosome complex RNA-binding protein Rrp4"
                     /protein_id="HSD04195.1"
                     /translation="MEVIKRRYVIPGDVITTGPYRPEQNVYQEGDRIISTSIGISEIY
                     ENAVRVIPLSGMYVPKTNDLVIGKVIGHSSLSWEFDINSCFIGFLPAQDVFGRDYSPT
                     KDELKARLDKGDLVAARVANFDRSRDPLLTVQDRDLGKIESGELITIAPSKVPRLIGK
                     RGSMIQTIEMATKAMITIGQNGLVVVSCEDPVGLLKAIEAVKMVDAQAHTPNLTERVK
                     SMLGVELGENNDTINE"
     gene            12749..13465
                     /gene="rrp41"
                     /locus_tag="VLB45_00385"
     CDS             12749..13465
                     /gene="rrp41"
                     /locus_tag="VLB45_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosome complex exonuclease Rrp41"
                     /protein_id="HSD04196.1"
                     /translation="MTQLMNENGIRCDGRRIDQIRNVSIKVGVLKNADGSAYIEFGKN
                     KILAGVFGPRDVHPKHMANQDTGIIRCRYHMSPFSVTERKNPAPSRREIEISKVIKEA
                     LQPAVILKDFPRTVVDVFIEVLQADGGTRCAALDAASVALVDAGIPMRDIVSACAAGK
                     VADKIVLDVNDEEDKEGQADMPVAYMPNLGKITLMQLDGILTPAEYKTCLDTAIGGCK
                     QVYELQRKALMEKYFESGEV"
     gene            13462..14268
                     /gene="rrp42"
                     /locus_tag="VLB45_00390"
     CDS             13462..14268
                     /gene="rrp42"
                     /locus_tag="VLB45_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000178 - exosome (RNase complex)
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exosome complex protein Rrp42"
                     /protein_id="HSD04197.1"
                     /translation="MTSSVIIEQLKREKILELLNQGKRLDGRGLHDQRPLSIEVGAIP
                     KANGSARVKLGDTEIVTGVKIQPDKPFPDLGDKGILICTAEILPLADPTAEPGPPSEE
                     VIELARVVDRGIRETEMIDLKQLVLIENKSVIGIFVDCSVINTDGNLFDACSYASVAA
                     ILSCTVPKFEIKDEVPVRVEGQTSKPPIKTIPVSVTMARIGDHIIVDPTADEEACMNA
                     RITITTNSDGNICALQKGGSDGFTVEQLHKCSEISLSVGTKIRKQLEQLM"
BASE COUNT         4553 a         2563 c         2644 g         4538 t
ORIGIN      
        1 aaaagtacaa agaactgcat tcattgttag aaccattagg aaaagaaatc aagtaaccaa
       61 cctccattca ctttttgaga aaacatattt cttgttgtgc gaaaaaatcg tatgtagata
      121 ccatctgcct actgattgat cgaattcata aactacatct gtaacatcag gtggaatctt
      181 ttgaggatca agttgatact gtaataattc taagatgaaa tcgattttct ctatagctat
      241 gctaaaccaa tgatgatcat caagatctgc tatgaatttt atatttggtt ttcctgaaaa
      301 atgtatgtat gtcttgatat ttttgcctcc acctctgcgt cttttcattt cttttagtat
      361 tcctttgatc ttgtcccatc tcacatgtcc aaaccatttg aaaaattcgt cactaaattc
      421 aagtggaaaa tctatcctta ttacagtaga gtaatcctct tctgattctg cactgggatc
      481 ttgttctact acaaacatgt catgtaacaa cccataaaga acttcaattt cccatgggga
      541 tactccataa tactttaggg atgctgtaag tgaatcctgc gtcactactt attctgagaa
      601 gccaatttga taaggctttc aatcttgttt tgtcctaaat ctagtttgat tcctgccttt
      661 ccgcaggtgt ctttttgata cttccatgtt ctcttatgaa attgtaatgt attcgataag
      721 cgtcaattag cttcttcttc agattcttca aaatcaaaat cactatttcc atacactgca
      781 aattttattg cctccgccac ataatcacta atagaacaac cgattttatt acatcgctct
      841 cttaactctg catgtaattg aataggtatg cgtgttgaaa cagttctaga caatcgttta
      901 catccgttta caagtatatt ctgatttaat taaacatcaa atttttgtaa tttaggtttg
      961 ttgattaggt aatagtgaat attaccggat tagaactaaa attaaagaaa aaatctgact
     1021 gatcatagta agaatattgt aatattttcg agtttagagt atctattaca aagataggtt
     1081 ttaagctgaa aatagcagat ttttcaaaaa agatgcatta aggcattctt aatttgtcta
     1141 aggtatcaag catatctaca attgactttt ccagatcaga aagcatgttt tctgcttttt
     1201 tatcaatctg actgccaaat gtctttctca agttttcaac agactttgca accacaaaaa
     1261 gcggttgtat tagatcatcc gtgttttttc ctgtgattat atgatcagct tcttttgatt
     1321 caaatgtctt gtatttcatt ttattcaacc ttgattgata cgcatgcact cattactcac
     1381 ataaaaattg ttataaaaat ggaataaaaa gatgtccacc caaaatagga aatgactagt
     1441 tttctataat ttattgttat caagtatttt tgcaaagaaa atttcataca gtatcacaat
     1501 ccgtctgcat ttgcgcaagt ttgacacatt ctatttctaa ttgccatatt tggaaatcat
     1561 tgtggtttat gtcacttgac cattatagga tatgttcaag atccttgaat gccttgataa
     1621 catgatttcc agttttggta acctcgtaat tcgtcctgac aggtctatcg cttgtaactg
     1681 tcctttctaa gagtcctagg taggtaagac cattaagtat tatagttata gaagctctgc
     1741 tgtccactat ttttttgtca attgcgtatt ttaatacctg attataaccg acattttctt
     1801 tgtcctttac atacaacaaa atttcataaa agcccttttt tgacatcatc ctaagaaaga
     1861 caaggaactc ctgtctctga ctgtcgctca acaccttgtt ggacactttt agaccagaaa
     1921 aagtcatggc atttgacatg tagtctaaca ctataaaata tagtgttaaa tactaatgtg
     1981 tgtctaagta atacggacaa ggttaagatg ctaatagata attgcatcaa aaaatgcaat
     2041 tttttcataa attcgtcatc taaaatgatg atttatcacg tatgtaacaa ttctaacata
     2101 cacacgattt tagcaaactt tgctatgagg caagtcacat ttgtctacta aaaatcaaat
     2161 gagaagagca ttggtaggtt tggctataga acaggctcta cttgacgcag gtggctccag
     2221 attgttaaaa gaagtaagta acataatgtt tgaagaattc gggtgtagta taccggattc
     2281 actttacaat cctgattatc taaataaggt gctcaaaaaa ctctttggaa attcgtatac
     2341 tgctattcta caatcgatta gaaaaaatct ggatgaattt agttatcagg aaccaattgc
     2401 agaattccta caagcagtcg gagcatagat cttgcaaaca agactggaaa cattacgaaa
     2461 acaaaaattt cttttcattg cactaatcat aactagttca cttgttgtct tagctaatca
     2521 gtttggagaa aaaatgtcaa tggtggttac tgatctcata tatggtccta taatcataat
     2581 actgactgtt ttggcaatca tcagtgcatc aacatctgat ctaaaaagtg atcacggaaa
     2641 ggcttggtta ttttttgttc tcggtgcaat atcgtgggca atagcagaac atctttggat
     2701 gatatatgaa cttgtacttg aagaagatcc gtttccctcc gcagctgatt ttttttacct
     2761 cggaggatat ccgttcgttt tcctgtttat gatatattat ctaaaattta ttaggacagc
     2821 aatttcaaaa aaaataattg tcgcagcaag ccttgtagct cttgcaattg gagctcctag
     2881 catttatctt tcctatgatc tggattcaga aattagcacc tttgatctga tactagcaac
     2941 tacgtacccg atagttgatg caatcatctt tgtacctgca atgataggcg taattctgtt
     3001 ttttagtggt agagtaaatt ttatgtggac tctcatgtgc ataggaataa tctcgcttac
     3061 tcttgcagat gccggttttc ttgcggcaga atatgatggt tcttactata ccggtcatcc
     3121 aatagacata cttttcatat ggacatacgt cctgttttca tttggcattt atgaccacat
     3181 caagattttt agagaaaaag tagtgcacaa aacacccatg actgagagaa atccgactga
     3241 gacataaccc tatttttcac agagaatact tcccgatctg tcatcctagt gcaaccaagg
     3301 cattacctag tcctatcggg attcgacttt taattgtaaa aaattttacc aattgaaggt
     3361 tctaaattgt ttgcagatat ttccataaat acaacacgcc gacagttcct attacaaata
     3421 ccatcggctt ccatgcaaat actggaattt ttggagttga aagctgaact ttatagttgt
     3481 caaaaatagc attaacatac ttctttattt tttcattcca aatcttgtat tctatttgat
     3541 gtttttttag tagtgcttct atttcataaa cgactggagt tctctgagag aaaaggtgtt
     3601 gtaaataatc tcgtgatact tcgacttctg catgtattgc aatcagtcct ttcttcatgt
     3661 ccaagagtct tacatgcatt tcccatggtt tttttaattt caaagaaaga ccattaccta
     3721 tctgaaatgg ttgtttgtgt tcaaatttga catgagtaaa tccttctttt gaaaatattc
     3781 ttaaaagttc atcaacattt tttctaacca caatagtaag ctgttccact cctgttttgc
     3841 tgtctacacc gacttttcct ttgaccccat caaaaaagga tattgtcttt gggtatctag
     3901 tcagaaactc taccatagct tgcggtccaa tttggccata ttaaaaccat aagtgtatca
     3961 aacatttgtc tttagtccac gaacatatac acactggtcg tgggatattg ccatctcgtc
     4021 ttggcttatt ttcctgtctg taattgcaac tctggagttt tttattactg caaacggagt
     4081 actgtctgaa ccctccccca tcttgagatt tgctattgat gcaagattgt ctgcaactgc
     4141 ctgaaaagtc actttgagtg gatttccata tagatccttt tgacctcgta tatcggagag
     4201 tggctctatt cctgcacaag ctattgcaac tccagttgtt cctattcttg caggcatcaa
     4261 cctgctgtct attatgatga ttcctatgtg tatagaaaat tttaggaaaa atttgcgttt
     4321 gagctgttca gcaactgcat atgcatcagt tggatacaaa atcaagcttc ccctttgtgc
     4381 atttgatttg tcaattcctg cattaggtgc caagatgttg tctgctgatg tgatgacaaa
     4441 acccggaact ccgccaaaaa ctgcatctga ttctcttaga ataatttcgg caattttcgg
     4501 attaagatga aactttgtag caagtttttg agattctaca gatggaataa cattttcata
     4561 ttttattatt cttccttgag agtttgctac atatttgctt gaaacaacaa gaacatctcc
     4621 attttcaagt ctaatatgag atctagctaa cgaatcaacc aatgtttcaa agagatcaaa
     4681 ctcttgcatt tttaattctg attttacagg gtgaattgag atagacacaa aaattctatg
     4741 gtgtttttac tttttagtct tcctgcaaac cttttaattt caaactagac tcatttttat
     4801 caaatgggca tgaacatcac tgaaaagata ctcgcaagag catctggtaa ctcaaaccta
     4861 tctccagatg atgtagtttt tgtaaatgtt gacaaggtaa tggtacacga tgtttcaggt
     4921 cctggagtaa tagctgtgtt tgaaaagcta aagaaaaaag gaatgaaaat agaaaagcta
     4981 tgggattcat ctaaagtctg ggttgcagaa gaccactttg ttccatctgc agataagatc
     5041 tctgcacaaa atgtagtatt gttatctgaa tttacaaaga cgtatggaat tgaaaagcat
     5101 ttcaaatatg gcatgggtca atatgggatc tgtcacactt tatcgcatga ggaagccatg
     5161 gtgcttccag gagaagtata tgtcggagga gattcgcaca caaatacaac tggtgcacta
     5221 ggtgcttttg cagcaggtct tggccacact gatgttgcat atgttctttt aaacggaaag
     5281 atttggttca aagttccaga aacattgtac tttaaactaa atggaaaatt acctagccac
     5341 gtaatggcaa aagatgtcat attgaaaata attggtgata taggtaccga tggtgcagct
     5401 tacaaaacaa tgcaatttgg aggcaatggc atttcagaaa tgtcagttga agagagactc
     5461 actttaacaa acatgacaac agaagctggc gcaaaaagtg gtatcataga accagatcaa
     5521 aaaataagag actatcttgc tatgcgaggt gcaacaaaat acaatgaagt tcatggcgat
     5581 cctgatgcgc aatatgaaaa aatctatgaa tatgaagcat cagaaatgga accaattgtt
     5641 gcaaaaccgt attctcctga gaacatttca ccagcaaggg atttgtctac aattcaattg
     5701 gacaaagcct acattggttc ttgtactgga gcaaaaatgg aggatcttta tgcggtggca
     5761 aaaattctca aaggcagaaa agtaaagatt agaaccgaaa tcttgccagc tgcaatatct
     5821 atatttagaa aggcgatgga tgagggattg ctaaaaattt tcatggatgc gggagttgta
     5881 gttggacctc ctacatgtgg agcatgctgc ggtgcacaca tgggtgtact tgcaaaagat
     5941 gaagtctgta taagcactac aaataggaat tttccaggac gaatgggcca catagactca
     6001 aaaacatatc ttgcttctcc acttgtagct gcagcatctg cagtgactgg gaaaataact
     6061 gatccgagag atttgaaatg aaaggtaatg caataaaata cgaaaaagat aacattgaca
     6121 ctgatgttat aattccagga ccatatctca aaatacatga ttacgtcgag cttgcaaaac
     6181 acgctatgga aggactagac aaagattttc attctaaagt gaaagaaggc gactttatcg
     6241 tagctgggcg taacttcgga tgcggttcaa gtagagaaca tgctcctata gctcttgcat
     6301 attctggcgt aaaagctgta ttggcattat catttgcaag aattttttat agaaatgctg
     6361 tagatggagc attcctactg cctatagaaa tagaggaaga tgcgtacaaa aatatctcag
     6421 acagagattc tatcgaaatt gatactgcta aaaatgaaat aaagaatctc accaaaaacc
     6481 aaatctataa aatgaaaccc ttttcagaat tgataggaaa gataattgct gctggcgggc
     6541 tttttaacta caagccataa aatataatga aagcagaata ctgataattt tgcttttata
     6601 ctattatatc tcgcatttgc cagactcttt atatgaaaaa aacactattt gagaaaatct
     6661 gggattctca tgttgtagtg gaacaaaatg gtggtcgttc attactttat gtggacagac
     6721 acttggtaca tgaggttacg tccccacagg catttgatgg attacgtatg aacaaaagaa
     6781 aggtacgaag accagatctt acatttgcaa caatggatca taatgtccca actagtgata
     6841 gatcattacc aatcatagac caaatctcgg caaagcagat tcaagtgttg gaaacaaact
     6901 gtaaagattt tggaattcag ctatttgaca ttcatagtcc aaatcaagga atagtacatg
     6961 tgataggtcc tgaattgggc attactctac ctggaactac gattgtttgt ggagacagtc
     7021 acacatcaac acatggtgca tttggtgcac tagcatttgg aataggtaca agcgacgtag
     7081 aacatgtgct agcaacacaa tgtatttgga tggaaaagcc aaaaactttt gaaataaaag
     7141 ttgagggaaa aagaaaaaat ccacatgcag ttactgcaaa agatatcata ctttcaataa
     7201 taagacagat tggtacatct ggaggaaatg gtactgtaat agaatatcgt ggagaagcta
     7261 tctctgatct tacgatggaa caaagaatga ccatatgtaa tatgtcaatt gaagctggcg
     7321 caagggcagg actagtagct cctgacgaaa aaacattcca ttatttaaga ggaagacgat
     7381 atgttccaaa aaactatgaa gaatatgtag attattggcg tgaaaatcta aaaaccgacg
     7441 aaggtgcaag tttcgataag agctttgtct tgcatgcaga aaaaattgtt cctcaggtaa
     7501 gctggggaac aaatcctgct atgactgtag atgttacagg tacagtgcca tttccagaag
     7561 aatatgcaaa aggaaatcct gaagaagaaa aagctgcagt caaggcatta caatacatgg
     7621 atctaaaacc gggagttcag ataactgaaa ttcacgttga ccgagttttc atcggttctt
     7681 gtacaaactc acgattggaa gatttgatcg aagcatctcg agtaataaaa ggtagaaaag
     7741 tctccccaaa agtacgtgcc atgattgtac caggttcaca attagtaaaa aaacaagctg
     7801 aagacttggg tttggataaa attttcaaag aagcgaattt tgaatggaga gaatctggat
     7861 gcagcatgtg tctaggaatg aatcctgaca tcttagctgc tggtgaaagg tgtgcaagca
     7921 cctctaaccg aaactttgaa ggtagacagg gtactggtgg aagaacacat cttgttagtc
     7981 ctgtgatggc tgccgcaact gcaattgcag gacattttgt agatgttaga gaaatggatt
     8041 tgaattaaaa atatggagcc atttaagact gtgaaaagca tcactactcc acttgataag
     8101 gtaaatgtag atactgatca aattgttcca aaacaatttc taaaactagt tcaaaagaca
     8161 ggatttggta aatatctgtt ctatgattgg cgatttacaa atgacggcaa accaaaagaa
     8221 gattttgttt tgaacaatcc cgcatataaa aattcgagaa tacttttagc tagagaaaac
     8281 tttggatgtg gctcaagtag agaacacgct gcatgggctt tacttgatta tggttttaga
     8341 gtagttatag ccccatcttt tgcagatatt ttctataata attgttttaa aaatggaatt
     8401 ctgccaatta cactcaataa taaaaccatg gatacgttat ttcgatttca aaatgaaatc
     8461 gaagttaatt tagaggatca gacaataaaa gttggtgata gtaaaatatc ctttgagata
     8521 gaaaatcata gaaaaaaaac actacttgaa ggattggatg atattgcagt taccttgcaa
     8581 tatgaggata aagtttcaga atatgaaaaa actagagaca aattctaatt cttcattttt
     8641 ctgattatgt ttttaattaa tttatcattt cgttcaactt tgattgggga ttcaatatct
     8701 acccatggtg ttttctctat gtcaaacgca ctcactgtat gtttttttga tagttcttga
     8761 agtacatcat atgacaagtt gatatttttt tttcttgttt ttttagctct gatcattttc
     8821 agaatatttg tttttaatat gtatatccca agacactccg gcatctctaa ttggattgtt
     8881 ggtttttcat taaattctaa aattcttccc tccttaacta ttgcatgtcc agtttcctca
     8941 gatctatatc ttctagttgc aatgcatgca atactctttg tctttttatg aaacttatac
     9001 atcattttaa gattgattgc acacaagttg tctacaaacc ataacgtaaa ttctccgtct
     9061 ttgatatgac ttagaagatg tactaggtct ccaccagttc cactgttgga atcttgaaca
     9121 aatttgattt ttttcagatc ttttctattt tcgaaataac ttttgatctg actcccaagg
     9181 ccattatagt ctgcaagaat gattatttct ttaataaaat caattgatga cagataccgt
     9241 actatgtaat ctatgagtgg ttttccctct attgggatca ttgcttttgg gaaatagtct
     9301 gtaaacggct tgcctcgtgt tcccgatcca cctgctagaa ttactgcttt caaactctat
     9361 ctgtatgatt tttgttttct tcgtctcgca ccaggtccgc caaacttctt tggttccttt
     9421 cgtctatcat ctccactcaa gagatgtttg tcaaattcat tcagacgctt cttgagatct
     9481 tcccgtattg tttttgtaaa tggatgatct tttggttctt ttttggtctt ggtccatcct
     9541 gtcattgctc ttgaaattgc tactgcactt gcatatgcct gtcccatgaa acctccgcca
     9601 ttgactctta cagaaagatc tactttttct cgtaaatctc ctgctatttc taaaggtgct
     9661 aatatgactt ctcttgcaat ttcttgttgt atcatttcta ctggaacgtt gtttattcta
     9721 actctaccat ctccctttga tatgtggaca tgagctcttg cagtctttct tgatgcgaaa
     9781 taattttcaa tcttaaccat tattcatgcc acccgaccat tctgcctaat tcaccaacac
     9841 tggtgtagta cggagaaggt tttgtaattt ttgcatcttc aaactgtgtt ctctctaatg
     9901 atctaagctc gtttggaact cccaaataca ctcttaatct cttgaatgct gcttgaccag
     9961 atgattttgt ttttggtagc atgcctctaa ccattttagt tatgatggta tctggtcttc
    10021 ttggatgaaa tggaccaaac cttggattgt taatgctagc aatttcgaga aattctcggt
    10081 atgactttat gattgcttct ctatttccac tgatcattat gttctcagaa tttactatgt
    10141 gcactctgtt tccttcaagc aaaagttttg caacacgtga acacaatctg ccagcaattt
    10201 gattagttcc atcaattatc atggtctgat ttcctttagc catcgttttt gtttctgctt
    10261 gctgtttagc caattatgat tactcctttt cctgttgggt gagtcttgat tagttctgtg
    10321 taactcataa tttttcctcc tgtctggatg attttctgaa caccagaatt tgaaatagaa
    10381 aacgaacaaa gtgtaatttt gtgtgacatg ttgccagttc caagtacttt gcctggcaca
    10441 actattgtat cattgtcctt ggttaatcta tcaatctgac tgatattgat ggttcttctt
    10501 gcagaggatg gtttcagtgc aagttctgca agtcttgacc aaatgggagc cttgttcttt
    10561 tttgatgcag acttcatctc ttttgccatt tgtaatacta tctgattagt catttgtata
    10621 attgccattt ttaacccaaa tataatgcat gctcagctac aaggctgcaa ttacttgctt
    10681 aaaaccttca agtctttttc ccaattcatc tactccggta agcactatct gagctggact
    10741 tagtgcacct gtagtttcaa cagtcaggag gcgatcttca gaatttcctg tttcggtaag
    10801 aactgataca gttgcagaat tccattttgc atgctccgtg ccccttccta atcgtgcata
    10861 tgcctcaaac ttgatctttt gtcctggtgc aagctctact atgggaatct tgtcattgac
    10921 tggtttcaca aagtcatctt ctgatgtaac ttcagcagta gttattgttc tggtagtgtc
    10981 tgcagaacca gaatcaacca tgagcatgac tctgcaatga gaacaaccaa cctcgctttt
    11041 acaatcgcaa tttgctggct ccacaaatct tgttaggtct gtttttagtg gaatcatggc
    11101 aagacgatgg gcaattccct catctgctaa tactgatgaa ttttcaatta ttgtaatgtc
    11161 atcaacggca aaaactggca ctccactaag acaaattctt cttaatgcat tagcatattg
    11221 tagaggtaca cctttcaatt ttacaacaat cttatgctca ttttgttcaa ctatttctaa
    11281 agaagccaag tcttaaagga aaaagttctt agtccaaata aaaatctagc gagtttttgg
    11341 gcataaataa ataccgaagt tgaatacctc cattatgacc gataaattta ccataattcg
    11401 attctctgta gaaggagaaa aatttgaact cttggtaaaa cctgatcccg ccttagagta
    11461 caaacttgga aaaagaaagg acgtttcaac cgttctagta tctgatgaga tttactctga
    11521 ttctaacaag ggcacacgtg catctactga gaaactcatg aaagcattca agaccaccga
    11581 tcaaactaca attgcaacta ttatacttca aaaaggtgat ctgaatctga ctactgatca
    11641 aagaagaaag atggtttcag aaaaacgaaa acaaattgta gactttattg caaaaacata
    11701 cgtagatcct cgttcccatt taccacatcc tccacttaga atagaacaag ccatggatga
    11761 tgcacgtgtt tcagttgatc cctttaaaaa tattgatgag caaacaaagg aaattgttga
    11821 acaactcaga tccattattc cgcttaaatc agaaaacatg ttacttgaaa ttcatgtacc
    11881 tgctcagttt gcagcacaat cttattctgt tttaaaatct gcgggaactt taaaaaaaga
    11941 agaatggcaa tctaacggtt cacttaaagt aatactagat ataccggctg cagcaagagc
    12001 aaatgtgata gagagattgg gatcggtaac gaaagggtct gcttctgttg aggtagccaa
    12061 atgatggaag taataaaaag aagatacgta attcctggtg acgtaataac aactggtcca
    12121 tacagaccag aacagaatgt ctaccaagaa ggagatcgaa tcatatctac aagtataggt
    12181 atatctgaaa tttatgaaaa tgctgtacgc gttattccac tatctggaat gtatgtacca
    12241 aagacaaatg atttggtcat tggaaaagtg ataggtcatt cttcactttc atgggaattt
    12301 gacattaact cgtgttttat aggatttttg ccagctcagg atgtatttgg aagagattat
    12361 tctcctacca aagatgagct aaaagccaga ctcgataaag gcgacttggt tgcagcaaga
    12421 gtcgctaatt ttgatagatc tagagatccc ttgcttactg tacaagacag ggatttagga
    12481 aaaattgaat ctggagaatt gataacaatt gctcccagca aagtcccgcg tttgattgga
    12541 aaacgtggtt caatgattca aacaattgag atggcaacaa aagccatgat tactattggt
    12601 caaaacggtt tggttgtggt ttcttgcgaa gatccagttg gattattaaa ggcaatagaa
    12661 gcagtcaaaa tggttgatgc ccaagcgcat acgccgaact tgactgaaag agtcaaatca
    12721 atgcttgggg tagaacttgg tgaaaataat gacacaatta atgaatgaaa atggaattag
    12781 atgtgatgga cgaaggatag accagattag gaatgtttct atcaaagtag gagtactgaa
    12841 aaatgcagat ggttctgcat acatagaatt tggaaaaaac aaaatccttg caggcgtctt
    12901 tggaccacgt gatgtacatc caaaacatat ggcaaatcag gacactggaa tcatacgttg
    12961 caggtatcac atgtcgccat tttctgtgac agagcgaaag aatcctgctc cttctagaag
    13021 agaaatagaa atctccaaag tgattaaaga agcattgcaa cctgctgtaa ttttaaaaga
    13081 ttttccaaga actgtcgtag atgttttcat tgaggtttta caagcagatg gaggtacaag
    13141 atgtgccgca ctagatgcgg catcagtagc tctggtagat gcaggaattc caatgagaga
    13201 tattgtttct gcatgtgctg caggtaaagt tgcagataag attgtcttgg atgtaaatga
    13261 tgaagaagac aaggaaggac aagcggacat gcctgtggca tacatgccaa atctcggaaa
    13321 aattacactc atgcaacttg atggaatctt gactcctgca gaatacaaaa cttgtctaga
    13381 cactgcaata ggtggatgta aacaagttta tgaactccag agaaaggcat taatggaaaa
    13441 atattttgaa agtggggaag tatgacaagc tctgttataa tagaacaact caaaagagaa
    13501 aaaatcttgg aactcttaaa ccaaggaaaa agacttgacg gtcgtggatt gcatgatcaa
    13561 cgtccacttt caattgaagt tggcgctatt ccaaaagcaa atggttctgc aagagtaaag
    13621 cttggagata cagaaatagt tacaggagtg aaaatacaac cagacaaacc cttcccagat
    13681 cttggtgaca agggaatttt gatctgtact gcagaaatac ttccattggc agatccaact
    13741 gctgaaccag gtccaccatc agaggaagtg attgaactag caagagtagt tgatcgaggt
    13801 atcagagaaa ctgagatgat tgatctaaaa cagctcgtcc taatagaaaa taaatctgtt
    13861 ataggcatat ttgtcgactg tagtgtgata aacactgatg gcaatctatt tgatgcttgc
    13921 tcatacgcgt ctgtcgcagc aattttgtca tgtactgttc caaaatttga aataaaagat
    13981 gaggttccag tgagagttga aggtcaaaca tcaaaaccac caataaagac aattcctgtt
    14041 tctgttacca tggctagaat aggagatcat attattgtgg atcctactgc agacgaagaa
    14101 gcatgcatga atgctagaat aacaatcaca acaaattctg acggaaacat ttgtgctcta
    14161 caaaaaggag gaagtgacgg atttacagtt gaacaactac acaaatgttc tgaaatatcg
    14221 ctttctgtgg gaacaaaaat aaggaagcaa cttgagcagc tgatgtagta tggcaaaaag
    14281 cagaagctca tcattgaa
//
LOCUS       DATIDL010000007         4640 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrosopumilaceae archaeon isolate SMAG_U16342
            k141_1225482, whole genome shotgun sequence.
ACCESSION   DATIDL010000007 DATIDL010000000
VERSION     DATIDL010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935836
            Sequence Read Archive: SRR25158326
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosopumilaceae archaeon (soil metagenome)
  ORGANISM  Nitrosopumilaceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrosopumilales;
            Nitrosopumilaceae.
REFERENCE   1  (bases 1 to 4640)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4640)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/04/2024 05:09:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,520
            CDSs (total)                 :: 1,474
            Genes (coding)               :: 1,474
            CDSs (with protein)          :: 1,474
            Genes (RNA)                  :: 46
            rRNAs                        :: 1 (5S)
            complete rRNAs               :: 1 (5S)
            tRNAs                        :: 43
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4640
                     /organism="Nitrosopumilaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1225482"
                     /isolate="SMAG_U16342"
                     /isolation_source="soil"
                     /db_xref="taxon:2202732"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            102..1310
                     /gene="ilvA"
                     /locus_tag="VLB45_00395"
     CDS             102..1310
                     /gene="ilvA"
                     /locus_tag="VLB45_00395"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - L-threonine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006549 - isoleucine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HSD04198.1"
                     /translation="MNLSFDDIVVSQKIQGNVIRRTPMDVSATFSQMTGSKIYLKSEC
                     LQKTGSFKVRGAYAKIYSLSKDEKKKGVIAASAGNHAQGVAFASALENIPCTIVMPVN
                     ASPAKVAATRAYGAKVVLEGTIYDESWSKALEISAKTGGQIIHAFDDQKVIAAQGVIG
                     LEIMQDLPDVDEIYLPIGGGGLAAGVLRAVKSKKPHVKVIGVQSSAFPAMKKSLDSGR
                     LESVEGKRTIADGISVKKPGEFTFKIINELIDDIVLVDDSQIVKAMFLLMERTKMVVE
                     PAGAVSLAYLLDKKPAKGKKVVPILSGGNVDMYLLGQIVAKGLTEMGRMVKISILLTD
                     KPGALKEVIDQIASLSVNIVEVIHDRLSSSIPVGTAGVTLSLETEDQKHAEKLINYLK
                     EKKIQFKIIT"
     gene            complement(1317..2648)
                     /locus_tag="VLB45_00400"
     CDS             complement(1317..2648)
                     /locus_tag="VLB45_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04199.1"
                     /translation="MPLQQLIESNNLREKISDIMIKNGLDFPCYSKILDYTISLFQAR
                     GLGIDYYGYHNIVHELEVTYVTLISAQWQSLQNYITKDDMKHLFVAALFHDFDPQKQV
                     DKPHEEDAVNFVKTDETLLELLKDAEVDANIVAALILRTTYPWIGELKQKREKQIDEY
                     FSASSLTKNDFEKIEHYKKLGWFLSVSDRIGGYALGNFSHALEMAKKNAHALAWHPNV
                     IARRSVAFFEDLLNNESEMCERVLQSLPREMRKIFMDNVLSFMRLREEEIQIKASLVY
                     ENVKLVPTIEPMSIRQDEEFIASLYKLYEELPYPLQFKKDSFSSSISNPNTILNTLRL
                     ENEKGPIVGFAKGGPLEDYKLRTEIQDRNYGKFNTIFLEPLALKTGYWGQKGGHEMRL
                     LFTMQAEAKSYRYLTSFALRDVIENRMKRNEEIEFVKQFDPERWDYYRIRL"
     gene            complement(2743..4137)
                     /locus_tag="VLB45_00405"
     CDS             complement(2743..4137)
                     /locus_tag="VLB45_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004421 - hydroxymethylglutaryl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006084 - acetyl-CoA metabolic process
                     [Evidence IEA];
                     GO_process: GO:0010142 - farnesyl diphosphate biosynthetic
                     process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HSD04200.1"
                     /translation="MTVGIDDIAIYIPRLFIDARDFAEARGMDPDKLQQGLGISKMAM
                     VDTNQDPACMAANACLRIMQRNKVSPKDIGRLYVATESSIDESKAMNSYVIGMLEQVY
                     GDGSFEHCGGIECKFACVSGSYALYDNSNWIRAGESGGKSAIVVVSDIAKYDLGSSGE
                     YTQGAGSVAMLVNDNPRLLEFDPKVTSTSIKNEYDFYRPFGKETPIVHGQYSNLLYLI
                     QVKKALMSYKEKALSTGLIKLKEGETILDHIDFLNMHLPYSNMGKKALAYLLRHEWRK
                     LPRWKKIIEQIGMQEPTPKDPRGTIESVLADAEFMTKDHEFTKKFVKTEEFQEAYDTK
                     LASSLIASQMIGNLYTASLYLGFRSSLEFEFQKGTDLDGKRIGFGSYGSGSSAMVFSG
                     VIRPEYKEIVKDMNLESEIGSRVKLSLEEYEEIHENKRGPRQNILESKKEFVLVDVEN
                     SPDNRGERRYVYNG"
     gene            complement(4243..>4640)
                     /locus_tag="VLB45_00410"
     CDS             complement(4243..>4640)
                     /locus_tag="VLB45_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HSD04201.1"
                     /translation="VKMIFKDFTIIGQDSINAANAAHCANEEGKFWEYHDMLYNNWTG
                     ENNGWASLENLAKFADDIGLNKAQFDSCMTEQRYFTIIESSNSDARTLGLTGTPGFFI
                     IGPDNTIAKIGGAQPYEVFEKTIESKLEK"
BASE COUNT         1373 a          885 c          819 g         1563 t
ORIGIN      
        1 ttcatgcctt gtggtttttg tacagggtgc ttgttctaag cctagaaatt cataggtgtg
       61 gcttggcaac atttatgttg taattataat gaaaaaacct gttgaatcta tcttttgacg
      121 acattgtcgt atcacaaaaa atccagggga atgtaattcg cagaacaccg atggatgttt
      181 ctgcaacgtt tagtcaaatg actggttcta agatttacct aaaatcagaa tgtttgcaaa
      241 agactggctc tttcaaagta agaggcgcat acgctaaaat ctattctcta tcaaaagatg
      301 aaaaaaagaa aggagtcatt gcagcctctg caggtaatca tgcacaaggt gttgcatttg
      361 catcagcact tgaaaatatt ccatgtacga ttgtaatgcc agttaatgca tcacctgcca
      421 aagttgccgc tacaagggcg tatggtgcca aagttgtact ggaaggtaca atatatgatg
      481 aatcatggtc aaaggctctt gaaatatctg caaaaactgg tggacaaata atacatgcat
      541 ttgatgatca aaaagtgatt gctgcacaag gagtaatagg actagagatc atgcaggact
      601 tgccagatgt ggatgaaatt tatctgccaa ttggaggagg cggacttgca gctggagttt
      661 tacgtgcagt gaaatctaaa aaaccacatg taaaagtcat cggagtacaa tcaagcgcgt
      721 ttcctgccat gaaaaaatca ctagattctg gaagactgga atcagtggag ggcaaaagaa
      781 ctatcgcaga tggaatctct gttaaaaaac caggagaatt cacattcaaa ataattaatg
      841 agttaattga tgatatagtg ctcgtagatg attcacagat agtgaaagcc atgtttttac
      901 taatggagcg aactaaaatg gttgtagagc cagcaggagc cgttagcctg gcatatctac
      961 ttgataagaa accagcaaag gggaaaaaag tggtcccgat tctttctgga ggaaatgtgg
     1021 atatgtacct gctaggtcag atagtggcaa aaggattgac tgagatggga agaatggtaa
     1081 aaatttccat cttgctaaca gacaaacctg gtgcgcttaa agaagtaatt gatcaaatag
     1141 catcacttag cgtgaacatt gttgaagtaa ttcatgatag attaagttcg agtattcctg
     1201 taggaacagc aggtgtgaca ttgagtcttg aaactgaaga tcaaaaacat gcagaaaaac
     1261 taatcaacta tttaaaagag aaaaaaatcc agtttaagat aataacataa ttgggtttac
     1321 agcctgattc tatagtagtc ccatctttct ggatcaaatt gttttacaaa ttcaatctct
     1381 tcatttcttt tcattctatt ttcaatgaca tctcgtaatg caaaactagt caaatacctg
     1441 tagctttttg cttctgcctg catcgtgaac aaaagacgca tttcatgacc tcccttttga
     1501 ccccaatagc cagtcttaag tgctaaaggc tctagaaaaa tagtgttgaa ctttccataa
     1561 tttctatcct gaatttcagt acgtaatttg taatcctcaa gtggaccacc ttttgcaaag
     1621 ccaactatag gtcctttctc attttccaat ctaagagtat taagaattgt atttggattg
     1681 ctaattgatg agctaaaact gtcttttttg aattgtaaag gatatggcaa ctcctcgtat
     1741 aatttgtata atgatgcaat aaattcctca tcttgtctta tgctcatagg ctctattgtg
     1801 ggaacaagct taacattttc atatactaga gaagctttga tttgtatttc ttcttctctt
     1861 aatctcataa atgacaaaac attatccatg aatatttttc tcatttctct tggaagtgat
     1921 tgtaatacac gttcacacat ttctgactcg ttgtttaaca gatcctcaaa aaatgctaca
     1981 gatcttcttg ctatcacgtt tggatgccaa gctaatgcat gagcattctt ttttgccatt
     2041 tctaatgcat gggagaaatt cccaagtgca tatccgccta ttctgtcaga aacagaaaga
     2101 aaccatccaa gttttttata atgttctatt ttttcaaaat catttttagt aagactggac
     2161 gctgaaaaat attcatcaat ttgcttttct cttttttgtt ttaattctcc tatccacgga
     2221 taggttgttc taagaattaa tgctgcaaca atatttgcat ctacttctgc atcttttaat
     2281 aattcaagta aagtttcatc tgttttcaca aagtttactg catcttcttc atgaggtttg
     2341 tcaacttgtt tttgtggatc aaaatcatga aagagggcag caacaaaaag atgcttcatg
     2401 tcgtcttttg tgatgtaatt ttgaagactt tgccactgtg ctgaaattaa ggtaacatat
     2461 gtgacttcta attcatgaac tatgttgtga tatccgtaat aatctatgcc cagacctctg
     2521 gcttgaaata aagaaattgt ataatctaat attttagaat agcaaggaaa atctaatcca
     2581 ttttttatca ttatgtcaga aattttctct cttaggttgt tggactcgat taattgctgc
     2641 aatggcacag tttttgcatt gggatgtcac ataaacattt tcttccttga ttcatcatac
     2701 gtgaggtgtt tgtgtattga aaaggatgta ttggtacaca cgctaaccgt tgtagacata
     2761 tcttcgctct cctctattat caggagagtt ttcgacatct accagtacaa attctttttt
     2821 actttcaagt atattctgtc gtggtcctcg tttgttttca tgaatttctt catattcttc
     2881 aagtgagagt tttactctac ttccaatctc tgattcaaga ttcatgtctt tgacaatctc
     2941 tttgtattct ggacgtatta ctccactaaa gaccattgcg ctactgccac ttccataaga
     3001 accaaatcca attcttttac catcaagatc agttcctttt tgaaattcaa attctagact
     3061 gcttctaaaa cctaagtaca gtgatgcagt gtaaagattt cctatcattt gtgatgcgat
     3121 aagagagctt gccagctttg tatcatatgc ttcttggaat tcctcagttt tgacaaattt
     3181 tttagtaaac tcgtgatctt ttgtcatgaa ttctgcatct gcaaggactg attcaatggt
     3241 accacgtgga tcttttggag ttggttcctg cattcctatc tgttcgatta tttttttcca
     3301 gcgtggtaat ttcctccatt catgtctaag caagtatgct agtgctttct ttcccatatt
     3361 actatatgga agatgcatat ttagaaaatc tatatgatct aaaattgttt ctccttcctt
     3421 tagcttgatt aatcccgttg aaagtgcttt ctccttgtat gacattagtg ctttttttac
     3481 ctgaattaag taaagcaaat tagaatattg tccatgtact attggagttt cttttccaaa
     3541 tggtctgtaa aagtcatact catttttgat tgaagttgat gtaacttttg gatcaaattc
     3601 caaaagccta ggattgtcgt taactaacat tgctacagac cctgctcctt gtgtatattc
     3661 accgcttgaa cccaaatcat actttgcaat atctgaaaca acaactattg cagatttacc
     3721 accagattca ccggccctta tccagttaga gttatcatac aatgcataag acccacttac
     3781 acaggcaaac ttgcattcta ttcctccaca atgctcaaaa ctaccatcac catagacctg
     3841 ctctagcata cctatgacat aggaattcat tgcttttgat tcatcaattg atgactctgt
     3901 agccacatat agacgtccta tgtcttttgg ggaaaccttg tttctttgca ttattcgtaa
     3961 acatgcattt gctgccatac atgctggatc ttgatttgta tccaccattg ccatctttga
     4021 aatccctagc ccttgctgaa gcttatctgg atccattccc cgtgcctctg caaaatcgcg
     4081 cgcatcaata aacagacgcg gaatgtagat ggcgatatcg tctatcccta ccgtcaacag
     4141 ctaccacctt cgggattgcg aatataagca ttgtggggtc aaaattgatc tcagcaaatt
     4201 gtaattttta ttcacattat cctattgatt gaaggaatta ttttattttt ctaacttcga
     4261 ctctatagtt ttttcaaata cttcgtatgg ctgcgcgcca cctatcttgg ctatggtgtt
     4321 gtctggacca atgatgaaaa acccaggcgt accagttaac cccagtgttc tagcgtcaga
     4381 attgctagat tcaataattg taaaatagcg ttgctctgtc atgcatgaat caaactgtgc
     4441 cttgttcagt cctatatcat cagcaaattt tgccaagttc tcaagtgagg cccatccatt
     4501 gttttcaccg gtccagttat tgtaaagcat atcgtggtac tcccagaatt ttccttcctc
     4561 attggcacaa tgtgctgcat ttgctgcgtt tatagaatct tgaccaatga ttgtaaaatc
     4621 tttgaaaatc attttgacct
//
LOCUS       DATIDL010000008         3297 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrosopumilaceae archaeon isolate SMAG_U16342
            k141_2032963, whole genome shotgun sequence.
ACCESSION   DATIDL010000008 DATIDL010000000
VERSION     DATIDL010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935836
            Sequence Read Archive: SRR25158326
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosopumilaceae archaeon (soil metagenome)
  ORGANISM  Nitrosopumilaceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrosopumilales;
            Nitrosopumilaceae.
REFERENCE   1  (bases 1 to 3297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3297)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/04/2024 05:09:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,520
            CDSs (total)                 :: 1,474
            Genes (coding)               :: 1,474
            CDSs (with protein)          :: 1,474
            Genes (RNA)                  :: 46
            rRNAs                        :: 1 (5S)
            complete rRNAs               :: 1 (5S)
            tRNAs                        :: 43
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3297
                     /organism="Nitrosopumilaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2032963"
                     /isolate="SMAG_U16342"
                     /isolation_source="soil"
                     /db_xref="taxon:2202732"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1939
                     /locus_tag="VLB45_00415"
     CDS             <1..1939
                     /locus_tag="VLB45_00415"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HSD04202.1"
                     /translation="AISENSEVYKFLESASRKYGIGFWKPGAGIIHQVVLENYAFPGG
                     LMIGTDSHTPNAGGLGMLAIGVGGLDAAETMSGLPWELLYPKRIGIYLTGELNGWTAP
                     KDIILYVASKLTVSGGTNSVIEYFGPGAKSISCTGKATITNMGAEIGATCSIFPYDER
                     MEVYLRTTDRGMLADLANKHKDLLIEDPEVEQNPEKYFDQVIRINLSTLEPHIVGPHT
                     PDLARPISQMAEDVKKNNYLDNISVALIGSCTNSSYEDMSRAANIAEQAKSRGIKTKI
                     PLLVTPGSEQIRATIERDGQMDSLKSIGATVLANACGPCIGQWNRPELKKGEQNTIVT
                     SYNRNFPGRNDGKRETMNFIGSPELVIALALGGKLSFNPLKESLVAPDGSKFKLDPPK
                     KAPEVPSKGFQKTEGIYVLPSEDPDSVEVIINKNSERLQKLEPFSQWDKNDFAELPIL
                     VKAKGKCTTDHISPAGPWLFYRGHLDRLSDNMFLGAVNAYNNEVGKGKNILTSSVQPF
                     PDIARQYKAKGIKWIVIGDRNYGEGSSREHAAMSPRFMGCAAVIVKSFARIHETNLKK
                     QGILALTFANHDDYEKIKETDRISLVELNRLEPGKPVKCFIHHENGTKEEILLNHSYN
                     SFQIQWFRAGSALNVLRSKEK"
     gene            2059..2658
                     /locus_tag="VLB45_00420"
     CDS             2059..2658
                     /locus_tag="VLB45_00420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04203.1"
                     /translation="MKITRQEIYGKAKVGTVAGLVGGFALFASLFGIDSQLDVTSGTF
                     YMMIGMAVGLHDMSAIAFGFIAHMLTAATIGAVFCVCSTLHRVLHLRSVWKGIFAGGI
                     TGLEVYVIFFMPITLFVMIPTLDATIIDGSKTIVTAQERISAVTLRENIDLIMWGALM
                     LHVLYGSIMGLFSGMVLHEDYKLTKKSAAEQESESMPAT"
     gene            2680..2916
                     /locus_tag="VLB45_00425"
     CDS             2680..2916
                     /locus_tag="VLB45_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010191878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04204.1"
                     /translation="MLGQKFDANALYNRIVHYYIDKKSYSKEKANAIAQTIVEREKLR
                     RICKNIKCKHSMDEHVRSQETCLILECECRHFVN"
     gene            complement(2913..>3297)
                     /locus_tag="VLB45_00430"
     CDS             complement(2913..>3297)
                     /locus_tag="VLB45_00430"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HSD04205.1"
                     /translation="IDRSKSDVISEVLKHTKNDGVDAVIDHVGAKTWPVSIDALKVGG
                     KMIACGTTTGTDAIVNIRMFYSKEAQIIGAYLGSKSQLATLHKFMKLKKIRPIIDSVF
                     ALKDARLAHKKMESSDQFGKIVLKI"
BASE COUNT         1118 a          581 c          644 g          954 t
ORIGIN      
        1 agcaattagt gaaaacagtg aagtatacaa atttttagaa tctgcaagca gaaaatatgg
       61 aattggtttt tggaaacctg gtgcaggaat cattcaccaa gttgttcttg aaaactatgc
      121 ttttccagga ggtctcatga ttggcaccga ctctcatact ccaaatgctg gaggtcttgg
      181 aatgcttgct attggtgttg ggggattgga tgcagctgaa acaatgtcag gattaccatg
      241 ggaacttctt tatccaaaac gtattggaat ctatctgact ggcgaactta atggatggac
      301 tgcaccaaaa gacatcatac tttacgttgc atcaaaactt acagtttctg gtggaacaaa
      361 ctctgtcatt gaatattttg gtcccggtgc aaaatcaata agctgtacag gtaaggcaac
      421 cataacgaac atgggcgctg aaattggtgc tacttgttct atatttccat atgatgaaag
      481 aatggaggta tatcttagga ctacagatcg tggcatgctt gcagatcttg caaacaaaca
      541 caaagatttg ctgattgaag atccagaagt tgaacagaac cctgaaaaat attttgatca
      601 ggtaattaga ataaatcttt caacactaga accgcatatt gtaggtccgc atacgcctga
      661 tctagctaga ccgatttcac aaatggcaga agacgtgaaa aaaaataatt atcttgataa
      721 catttcagtc gcactgatag gaagttgtac aaactcttca tatgaagaca tgtcacgtgc
      781 tgcaaatatc gcggaacaag caaaatctcg aggaattaaa accaaaatcc cactacttgt
      841 gactcctggt tcggagcaaa ttagagctac aatagagcgt gacggtcaga tggattctct
      901 caaatctatt ggggcaactg ttcttgcaaa cgcatgtggt ccatgtatag ggcaatggaa
      961 taggcctgaa ctcaaaaaag gagaacaaaa tacgattgtc acctcatata atagaaattt
     1021 cccaggaaga aatgacggaa aaagagaaac gatgaacttc attggaagtc cagaattagt
     1081 catagcgctt gctctaggcg gaaaactctc gtttaatcca ctaaaagaat cacttgtagc
     1141 accagatggt tccaagttta aattagatcc tccaaagaaa gcacctgaag tacccagtaa
     1201 aggatttcaa aaaactgaag gcatctatgt cttgccttct gaagatcctg attcagttga
     1261 agtcataatt aacaaaaaca gtgaaagact gcaaaaatta gaaccatttt cacaatggga
     1321 taaaaatgat tttgcagaac ttccaatatt ggtaaaagca aaaggaaaat gtactacaga
     1381 tcacatatcc cctgcaggtc cttggttatt ttatcgtggg catcttgaca ggctaagcga
     1441 taacatgttt cttggtgcag taaatgctta caacaacgaa gtaggaaaag gcaagaacat
     1501 acttacttca agtgtacagc catttcctga cattgcaaga caatacaaag ctaagggaat
     1561 aaaatggata gttatcggag atcgtaacta tggtgaagga agtagtagag aacatgccgc
     1621 aatgtctcca agattcatgg gatgtgctgc tgttattgta aaaagttttg ctcgcataca
     1681 tgaaaccaat ctgaaaaagc aaggaatact agctctcact tttgcaaatc atgatgatta
     1741 tgagaaaata aaagagactg acagaattag tcttgtagaa ttaaatcgtc tggaaccagg
     1801 aaaaccagtc aagtgtttta ttcatcatga aaatggaaca aaagaagaga ttctacttaa
     1861 tcactcttac aacagcttcc agattcaatg gttccgtgct ggttctgctc tgaatgttct
     1921 acgctcaaaa gaaaaataac caatttttat acaaaatctg gtttataact gaagattttt
     1981 ccatttatta aaaaataaaa gcgactaata attaaaaatc tccaataaat taaaatattt
     2041 tgaagtgtat aatgatacat gaagattact cgtcaagaga tttacggtaa ggctaaagtt
     2101 ggaaccgtgg ctggtcttgt aggggggttt gctttattcg catccttgtt tggtatagat
     2161 tctcaacttg atgttacatc cggaactttc tatatgatga ttggaatggc agtaggatta
     2221 catgacatgt ctgcaattgc ttttggattt attgcacaca tgcttactgc tgcaacaata
     2281 ggagctgttt tttgtgtttg ttctacatta cacagagtat tacatctaag aagtgtatgg
     2341 aagggtatct ttgctggtgg aataactgga ctagaagtgt atgtgatatt ttttatgcca
     2401 attacattgt ttgttatgat tccaacactt gatgccacaa ttatcgatgg aagtaagaca
     2461 atagtgactg ctcaagagag aatatctgca gtaacactgc gtgaaaacat tgatctaata
     2521 atgtggggtg cactaatgtt acatgtacta tacggatcaa taatgggatt gtttagcgga
     2581 atggtgttac atgaagacta caaactcaca aaaaaatcag ccgccgaaca agaatctgaa
     2641 tctatgccag ctacatagcg ataatatttt gtgattgata tgttgggtca aaaatttgat
     2701 gcaaacgcgc tctataacag aatagtacac tattatatcg acaaaaaaag ctattccaaa
     2761 gaaaaggcaa atgccatagc tcaaactata gtggaaagag aaaaactacg aaggatctgt
     2821 aagaacataa aatgcaaaca ttcaatggat gagcatgtaa gatcccagga aacttgtctt
     2881 atcttagaat gtgaatgcag acattttgtt aattagatct tgagaacaat cttgccaaac
     2941 tgatcactag attccatttt tttgtgcgct aatctggcat cctttaaagc aaaaacacta
     3001 tcaattatgg gtcgtatttt ttttaatttc atgaatttgt gaagtgtcgc aagttgtgat
     3061 tttgaaccca aataggctcc tatgatctgt gcctccttgc tgtaaaacat ccgtatgttc
     3121 acaatagcat cagtgccagt cgtagtacca catgcaatca tctttccacc cactttcaat
     3181 gcatctatac ttactggcca tgtttttgca cccacatgat caataactgc atctactcca
     3241 tcgttttttg tatgcttgag aacttcagaa attacatctg atttacttct atcaata
//
LOCUS       DATIDL010000009         7460 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrosopumilaceae archaeon isolate SMAG_U16342
            k141_15805, whole genome shotgun sequence.
ACCESSION   DATIDL010000009 DATIDL010000000
VERSION     DATIDL010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935836
            Sequence Read Archive: SRR25158326
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosopumilaceae archaeon (soil metagenome)
  ORGANISM  Nitrosopumilaceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrosopumilales;
            Nitrosopumilaceae.
REFERENCE   1  (bases 1 to 7460)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7460)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/04/2024 05:09:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,520
            CDSs (total)                 :: 1,474
            Genes (coding)               :: 1,474
            CDSs (with protein)          :: 1,474
            Genes (RNA)                  :: 46
            rRNAs                        :: 1 (5S)
            complete rRNAs               :: 1 (5S)
            tRNAs                        :: 43
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7460
                     /organism="Nitrosopumilaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15805"
                     /isolate="SMAG_U16342"
                     /isolation_source="soil"
                     /db_xref="taxon:2202732"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..111)
                     /locus_tag="VLB45_00435"
     CDS             complement(<1..111)
                     /locus_tag="VLB45_00435"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HSD04206.1"
                     /translation="MNSYQCHQVLGVKSDAPFKEIKSAYRRLALEYHPDKN"
     gene            complement(173..1498)
                     /locus_tag="VLB45_00440"
     CDS             complement(173..1498)
                     /locus_tag="VLB45_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008298513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle receptor subunit
                     alpha"
                     /protein_id="HSD04207.1"
                     /translation="MLDVLKTGLRAALKKIVNSSSIDEALIKDLAKDIQRALLQSDVN
                     VKIVFEITKNLEQRSLNETPPPGLSRKDHIVKILYDELAKLLGVEKQFEFQAGKTNKV
                     LMLGIQGSGKTTITAKLSKFLTRQGYRVAVVGADTYRPGALVQLKTMCEKANIEVYGE
                     ENNKDSPDIVKNGLKHFENSNYDIILIDTAGRHKEEKDLLAEMTQINKVANPDLALLV
                     IDGTIGQQCYNQAEAFHKIVPVGGIVITKLDSSAKGGGALAAAAATGAQIMYIGTGER
                     IDDLEKFSPTRFVGRMLGMGDIQALLDMAKRLEDGSDEERLKRMSHGKMNMEDFYFQI
                     EEATKAGGLRNILDSMPGFSGMVKEDQLDQQEERMQKWRFIIQSMTKQEKADPDLLNA
                     SRVKRIARGSGWSEHDVKELLKAYKNSKDMMKASKGRQMQGMLRKMGLG"
     gene            complement(1499..2182)
                     /locus_tag="VLB45_00445"
     CDS             complement(1499..2182)
                     /locus_tag="VLB45_00445"
                     /EC_number="6.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017178 - diphthine-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0018193 - peptidyl-amino acid modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphthine--ammonia ligase"
                     /protein_id="HSD04208.1"
                     /translation="MNLAALFSGGKDSTFAIYKAKLEGHNVRCLVTVFPVSDESQFLH
                     FPNISITKLQAKSMGLPHLFHQTTSTDVQTEMNEIEKLLAQAKNDFGIEGLVHGGILS
                     EFQKTRFENICSKLNLKTISPLWKTDQKEYMKNLLDSKFKFIITSVSSAGLDDSWLGK
                     EITKDDLQKLDTLSVKHGFNLSFEGGEAETLVLDCPLFSSPIKIVKSQKTWDGYRGRF
                     EITEAILDQ"
     gene            complement(2457..2864)
                     /locus_tag="VLB45_00450"
     CDS             complement(2457..2864)
                     /locus_tag="VLB45_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-5A"
                     /protein_id="HSD04209.1"
                     /translation="MSKPSELGSLKIGSYILLPVDSPNAEPCKIVEFDTSKPGKHGAA
                     KARIVAVGIFDKKKYPHVSPVSAQVQVPLIDKRVGSVISKTESIIQVMDSETFEVFDV
                     SQIEDEIKDKVAQGQDVEYWKVMDRTIIVRIKS"
     gene            complement(2900..3502)
                     /locus_tag="VLB45_00455"
     CDS             complement(2900..3502)
                     /locus_tag="VLB45_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04210.1"
                     /translation="MSAVGEGYVCAPYLHDHSSIELKDSWEKSKDVDGMYFVTATFSQ
                     DSSAYFPSRSNHYILARFQNAKKMMKEIEKYKQDKITFVFTMDGELFQRNMEDKLNFV
                     SVYYLEYGQSDDDIRDIATIVAKREKVRRAGLVHLDLSANEVPKFTFPYNKNLVVLEV
                     SSDKSHQSNNKYCEKTRRDVGRKGITMTNLVSLSVLDKLK"
     gene            3657..3779
                     /locus_tag="VLB45_00460"
     tRNA            join(3657..3694,3741..3779)
                     /locus_tag="VLB45_00460"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3691..3693,aa:Tyr,seq:gta)
     gene            3821..4324
                     /locus_tag="VLB45_00465"
     CDS             3821..4324
                     /locus_tag="VLB45_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HSD04211.1"
                     /translation="MSGESWSNLDKVDQKIIAILNKNARTPSKEIATELKNSGLDVSD
                     RTIRKRIERLEKNGIIKGYKAVLSDVSGSDEFEALFLKFRITRSLDTIKESIKEFVKT
                     LPNYLFIASLEVEWNMIIVMKVDEMQKNPSSKIVEKFSEQILDYRISEIQIKDINLLN
                     MSLLLVS"
     gene            complement(4321..5064)
                     /locus_tag="VLB45_00470"
     CDS             complement(4321..5064)
                     /locus_tag="VLB45_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008298214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSD04212.1"
                     /translation="MAEKEIRQKYDLACTYLKNKQTSSAHGLFLDLAANEEKKESYKA
                     GLFFLLAAECKIQQGKDGYDEILKAGNLYLSLAKKEKSQHAKNAYLCAAKCFLKIGQY
                     EVAKNAFEKSKKFVSTVVENKRPIVVVDDSPAIIMKISSHLEKLGYKDVHAFKTGKEA
                     LSACKKLIQSSQDPIVLLDMGLPDIAGDVVATKLLHDKPDLSIILITADEKTSKRVHK
                     TISLGATAFIQKPFGIDDLKDALATVEFG"
     gene            complement(5106..5735)
                     /locus_tag="VLB45_00475"
     CDS             complement(5106..5735)
                     /locus_tag="VLB45_00475"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HSD04213.1"
                     /translation="MSKIISSASNVGALLAMPIGKLMDTDALIMEGDKTVFDAIKQMQ
                     AKGAKVVLISHQGEAIGIVSKSDMLFKVAAKNLPLNKIKLREIMTSPVYSLPPNSSLQ
                     DALDYMDKYKIKQIIVSTGSSILGIMRRSSIYEKVQSASLSTVESLTEGAPMCIINPN
                     AVKYMKDLSNAKAVCPYCESPFDDKTALSKHIDRLHGGAGLLEGDVRQY"
     gene            complement(5862..6914)
                     /locus_tag="VLB45_00480"
     CDS             complement(5862..6914)
                     /locus_tag="VLB45_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HSD04214.1"
                     /translation="MKAAQIVTPKEPLQLNDLQVPKPKDAEVLVKVKAVGVCHSDLHL
                     WEGGYDAGAGSFLKATDRGVKFPVTPGHEVVGTVTEIGSSVQGTKVGDEVLVYPWIGC
                     GVCPACKVGNENLCDAPRSLGVFQNGGYAEYVLVPSFKYLIKISGIDLEGAASLACSG
                     LTAFTAVKKANVGPGEFLVIVGAGGLGLMGIQIAKAITKATILCVDLDDNKLAAAKKM
                     GADHTINSKDPDTAQKIISVCNNKGPECVIDFVNAPPTVKMDLAVLRKRGNLVLVGLF
                     GGSAELVLPTIPLKAITIQGAYTGNYKNLVELVELAKRGVINPIVSRRYSLDDANTAL
                     EELKKGKIIGRAVINP"
     gene            complement(6956..7123)
                     /locus_tag="VLB45_00485"
     CDS             complement(6956..7123)
                     /locus_tag="VLB45_00485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04215.1"
                     /translation="MEQINASCPRCNSDGLQYTSYKAPLKILKQRSWLYCKPCGYEIE
                     MEDFKRSLFCP"
     gene            7256..>7460
                     /locus_tag="VLB45_00490"
     CDS             7256..>7460
                     /locus_tag="VLB45_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010195514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein ScpA"
                     /protein_id="HSD04216.1"
                     /translation="MSEENSGTGISQPPINVLFNPIDISKKDVWAIDIIRILELLIKI
                     LNQTGKKDLRVAGIAALSSAMIHR"
BASE COUNT         2027 a         1529 c         1380 g         2524 t
ORIGIN      
        1 gtttttgtca ggatgatatt ctagcgccaa tcttctgtaa gcagatttta tttccttaaa
       61 tggtgcatcg ctttttacac ctaaaacctg atggcactgg tagctattca ctgattagta
      121 tgaagcagaa ctttgttaaa aataattaga taccactagt ttcatgcaga gactaaccca
      181 gtcccatctt tctaagcatg ccttgcatct gtcttccttt ggaggccttc atcatgtctt
      241 ttgaattttt gtaggccttt agaagttctt ttacgtcgtg ttcggaccaa ccagaacctc
      301 ttgcgattcg ttttactcgc gatgcattca acagatcagg atctgctttt tcctgttttg
      361 tcatggactg tattatgaat cgccattttt gcatccgttc ttcctgctgg tcaagctgat
      421 cttcttttac cattccagaa aagccaggca tgctatccaa aatgttgcgc agtccgcctg
      481 cctttgtagc ctcttcaatt tgaaagtaaa aatcttccat gttcattttt ccatgagaca
      541 ttcgttttaa tcgctcttca tcacttccgt cttcaagtct ttttgccatg tccaaaagag
      601 cctgaatgtc tcccattcca agcattctac caacaaatct cgtcggagag aacttttcaa
      661 gatcatcaat tctttcccct gtaccaatgt acattatctg agctcctgtt gcagctgctg
      721 cagccaatgc tcctcctccc ttggcactgc tgtcaagttt tgtaattaca atgccaccaa
      781 ctggcacgat tttgtgaaat gcttctgctt gattgtaaca ctgctgccct attgttccat
      841 ctattacaag caatgcaaga tctggatttg ctactttgtt tatttgcgtc atttctgcta
      901 gcaggtcttt ttcttctttg tgtcttccag ctgtatcaat cagaattata tcataattag
      961 aattctcaaa gtgtttcaaa ccatttttaa caatgtctgg agaatctttg ttgttttctt
     1021 cgccataaac ttctatgttt gctttttcac acattgtttt tagctgtact agtgctcctg
     1081 gcctgtatgt atcagctcca acaactgcaa ctcggtatcc ttgtcttgta agaaacttgg
     1141 agagttttgc agtgattgtt gtctttccac ttccttggat tccaagcatc agcaccttgt
     1201 ttgtctttcc agcttggaac tcaaactgtt tttctacgcc aagcagtttg gcaagctcat
     1261 catacagtat ctttacaatg tggtcttttc gtgaaagccc tggaggcgga gtttcgttta
     1321 gagaacgttg ttccagattt tttgtaatct caaagactat ctttacgtta acatcagatt
     1381 gtaagagcgc cctttggatg tccttggcaa gatcctttat gagtgcctca tctatggatg
     1441 aggaatttac tatctttttt agagcggcac gaaggccggt ctttagaaca tctagcattt
     1501 actggtctaa gatggcttcg gttatttcaa atcttcctct gtatccatcc caggttttct
     1561 gagattttac tattttaatt ggagatgaga aaagcgggca gtcaagaacc aaagtttctg
     1621 cctccccacc ttcaaagctt agattaaacc catgttttac tgaaagggtg tcaagttttt
     1681 gcagatcatc ttttgtgatt tcttttccaa gccaagaatc atcaagacct gcggacgaca
     1741 cacttgtgat tataaattta aattttgaat ctagcaaatt tttcatgtat tctttttgat
     1801 ctgtcttcca caatggtgaa atggttttta aatttaattt agaacagata ttttcaaaac
     1861 gagttttttg aaactcgctt aatattcccc catgcacaag accttcaatg ccaaaatcat
     1921 tttttgcttg tgctagtagt ttttctattt cattcatttc tgtttgtaca tcagtagaag
     1981 ttgtttgatg aaacaaatgc ggcagtccca tagattttgc ctgtagtttt gtaattgata
     2041 tgtttgggaa atgaagaaac tgactttcat ccgaaactgg aaaaacagtt accagacatc
     2101 taacgttatg gccttcaagt tttgctttgt aaatcgcaaa agtgctatcc ttaccacctg
     2161 aaaataatgc tgctagattc aatttgtcgt caaagggttt tgttgtaaat taattgcttt
     2221 gcagtttggt tacttgatta agacattttc cgcacaacga aaggctgtcc cgaattgtaa
     2281 cctcaacgtt tgaagacagg catacgtgac atagcccttt catacattat gtatggtata
     2341 acttgggtat atcttattcg catattttat tcgtattaga aaagcctgga tgcttcaata
     2401 ctcataatct tcatcatgaa tcagatggct tttctcctca tcatcagcat ccaaagctag
     2461 cttttgattc ggactataat agttctatcc atgaccttcc agtattcgac atcttgtcct
     2521 tgtgcaacct tgtcttttat ttcatcttca atttgactta cgtcaaacac ttcgaaggtt
     2581 tcagaatcca ttacttgtat tatagattca gtctttgaga ttactgaacc aactcttttg
     2641 tcaatcagtg ggacctgcac ttgtgcacta actggtgaca cgtgtggata ttttttcttg
     2701 tcaaatattc ctacggcaac aattcttgct tttgcggctc catgttttcc tggtttactt
     2761 gtatcgaatt caactatttt acacggttca gcgtttggac tgtccaccgg taacagtatg
     2821 taagatccaa tttttaacga accaagctct gatggtttgc tcattagaaa aacacaattc
     2881 ggctgtgtat ttaacttttc tattttagct tatcaagaac ggaaaggctt acaagatttg
     2941 tcattgtgat cccttttctg ccaacatcac gccttgtttt ttcgcagtac ttgttattgc
     3001 tttgatgact cttgtcactt gacacttcaa gtacaaccaa gtttttgttg tatggaaatg
     3061 taaacttggg aacttcattt gcagacagat caaggtgaac aagtcctgcg cgcctgacct
     3121 tttctcgctt ggcaactatt gttgcaatgt cccttatgtc atcatcagac tgaccatatt
     3181 ccaagtaata cacggaaaca aaatttagct tgtcctccat gtttctttga aatagctctc
     3241 catccatggt gaaaacaaaa gtaatcttgt cttgcttgta tttttcaatc tctttcatca
     3301 tttttttggc attttggaat ctggcaagga tatagtggtt tgagcgtgaa ggaaagtagg
     3361 cactgctgtc ttgggaaaag gtagctgtta caaaatacat gccgtctaca tctttggatt
     3421 tttcccagct atcttttagc tcaattgatg aatgatcgtg caaataggga gcacaaacat
     3481 agccctcacc cactgctgac atcccagaca caaaattgtt gatcaaccca gtttcttata
     3541 tagtgttagt ctgatcgaac aattttttga tttgaatgtg taatttgcaa attatttcaa
     3601 tttagattca aaccatcgat agttatttaa tacgaaaata cagcgtgaag tttagtccca
     3661 cgctagctca gcctggtaga gcttccggct gtagtggttg gcttttggct aactgatgtc
     3721 agcctatcag gcgtacagaa accgggttgt cgcaggttca agtcctgcgc gtgggaccat
     3781 taacataaaa ctcaaaagca gatcaaactg tataaaacca atgtcaggtg aaagctggtc
     3841 aaatttagac aaagttgatc agaaaattat tgcaatacta aacaaaaatg cacgaacgcc
     3901 atcaaaagaa attgcaacag aactaaaaaa ttctggtctt gatgtctctg acagaacaat
     3961 aagaaagaga atcgagcgtt tagaaaaaaa cggaatcata aaaggataca aggcagttct
     4021 cagtgatgta tccggctcgg atgaatttga ggcgttgttt ttaaaattta gaatcacaag
     4081 atcgcttgac acaataaaag aatcaatcaa ggagtttgta aagaccctgc caaattatct
     4141 gttcattgca tcgctagaag ttgaatggaa catgatcata gtcatgaaag tagatgaaat
     4201 gcagaaaaat cctagctcaa aaatagtaga aaaattctca gagcagattt tggattatcg
     4261 cataagcgag attcagatta aagatatcaa tctgctcaac atgtcactct tgcttgtgtc
     4321 ctagccaaat tctacagtag caagcgcatc ctttagatca tcgatgccaa atggtttttg
     4381 gatgaacgca gttgccccca agcttatagt tttgtgaact cgctttgagg ttttttcatc
     4441 ggcagttatc aggataattg acagatcagg cttgtcatgc agcaatttag ttgctacaac
     4501 gtcccctgca atgtcaggca atcccatatc aagcagaact atagggtcct gtgatgattg
     4561 aattaacttt ttgcaagctg atagcgcttc ttttccagtt ttaaaagcat gtacatcttt
     4621 gtaaccaagc ttttccagat gactagatat tttcattatt attgcagggc tgtcatcgac
     4681 aacaactatg ggtctcttgt tttctactac agtagagaca aacttttttg atttttcaaa
     4741 tgcgtttttt gcaacttcgt attgaccaat ctttagaaaa cattttgcgg cgcaaagata
     4801 agcgtttttg gcgtgttgtg atttttcttt ttttgcaagg ctgagataga gatttccagc
     4861 ttttagtatt tcgtcataac catccttgcc ttgctgaatt ttgcattcag cggcaagcag
     4921 gaaaaacagt ccagctttat agctttcttt tttttcttcg tttgctgcaa gatctaaaaa
     4981 caggccgtgt gctgaagaag tctgtttatt tttaagatac gtgcacgcaa ggtcgtattt
     5041 ttgcctgatc tctttttctg ccaagttatt gaaaataaga aaaatccgct tattatttta
     5101 aattgtcagt attgtctgac gtctccttct aagagacctg ctccgccatg tagtctgtct
     5161 atgtgttttg agagtgctgt cttgtcatca aacggagatt cgcagtatgg acagactgcc
     5221 tttgcattgc tcaaatcttt catgtatttc acagcatttg gatttatgat gcacattggt
     5281 gcaccttcag taagagactc tactgttgac agggatgcgg actggacttt ttcatagatg
     5341 cttgagcgtc gcataattcc cagaatcgaa gagcctgtgc tgacaattat ttgctttatc
     5401 ttgtacttgt ccatgtaatc aagcgcatct tgcagactcg aatttggggg aagactgtaa
     5461 actggactgg tcattatttc gcgcagttta attttgttta gtggtaagtt ttttgcagcg
     5521 accttaaaca acatgtcaga tttgctaact attcctatgg cttcaccctg atgcgagatc
     5581 aggacaactt tggctccttt tgcttgcatt tgttttatgg cgtcaaagac agtcttgtct
     5641 ccttccatta tcaatgcgtc agtatccatc agtttaccaa ttggcattgc taatagtgcg
     5701 ccaacattag aggcagaaga aattatttta ctcacgtaaa ctaattgcta atcttttatt
     5761 taaattttga aagatgggtt tactctatga ataatttctt aaaaaacaat ttttaacaca
     5821 taatgatgat aaaatttaga attaatcgta tcagttgctt tttatggatt tatcactgcg
     5881 cgtccgatga tttttccttt tttcaattcc tcaagagcag tgtttgcatc atcaagcgaa
     5941 tatcttcttg agactattgg gtttatgacg ccgcgttttg caagttcaac tagttctacc
     6001 aaatttttat agtttccagt gtacgcgcct tgaattgtga ttgcctttag cggaattgtt
     6061 ggcaagacaa gttctgcaga tcctccaaac aatccgacaa gaacgaggtt tccacgtttt
     6121 cgcaaaactg caagatccat ttttactgta ggtggcgcat ttacaaaatc aatcacgcat
     6181 tctggtcctt tgttattgca aacagaaatt attttttggg ctgtatctgg gtcttttgaa
     6241 tttattgtgt gatctgcccc catctttttt gcggcagcaa gtttgttgtc atccaaatcc
     6301 acacaaagaa ttgttgcctt ggtgattgcc ttggcaattt gtatgcccat cagtcccagt
     6361 cctccagcac caactattac aagaaattcc ccaggtccca cgtttgcttt ttttactgca
     6421 gtaaatgcag tcagacctga acaggcaagc gatgccgccc cttcaagatc aatgccagaa
     6481 atttttatta gatatttgaa acttggaacc aagacatatt ccgcataacc accgttttga
     6541 aatacaccta gcgaacgcgg agcatcgcaa agattttcat ttcctacttt gcatgcagga
     6601 caaacgccac atccaatcca cggataaacc agaacttcat ctccaacttt tgttccctga
     6661 accgaacttc caatctcagt tacggttcca accacttcat ggcctggagt gacaggaaac
     6721 ttcacacctc tgtcagttgc ttttagaaaa gaaccagcgc ctgcatcata tcctccctcc
     6781 cagaggtgca agtcgctgtg acaaacaccc actgccttta ctttgactag aacttcggca
     6841 tcttttggtt ttggaacttg gagatcgttt agttgcagag gctctttagg agtgacaatc
     6901 tgggctgcct tcatggcagt agtgtttttt gtttaataat ataagagttg actgttcaag
     6961 ggcagaaaag tgatctttta aagtcctcca tctcgatttc gtatccgcat ggcttgcagt
     7021 aaagccaact gcgttgtttt aggatcttta atggagcttt gtagcttgta tattgcaagc
     7081 catcactgtt gcatcttgga catgacgcgt ttatttgttc catctgataa aactaggatt
     7141 tttttgcata taaaatccgc gtagaattga ttagatcaaa tcatccttaa ttgaccagct
     7201 cttggaaaaa agtagagcgg ttagatatta taaacaaaaa atgattttta gaatagtgag
     7261 cgaagaaaat tctggtaccg gcatttcgca gcctccaatc aatgtgctct ttaatcccat
     7321 agacatatca aaaaaagacg tctgggcaat tgatataata cgaatcttgg agctgctaat
     7381 caaaattcta aatcagacag gcaagaaaga tcttcgagta gcaggaattg cagccttgtc
     7441 ttctgcaatg atacacagga
//
LOCUS       DATIDL010000010         3982 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrosopumilaceae archaeon isolate SMAG_U16342
            k141_1632655, whole genome shotgun sequence.
ACCESSION   DATIDL010000010 DATIDL010000000
VERSION     DATIDL010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935836
            Sequence Read Archive: SRR25158326
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosopumilaceae archaeon (soil metagenome)
  ORGANISM  Nitrosopumilaceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrosopumilales;
            Nitrosopumilaceae.
REFERENCE   1  (bases 1 to 3982)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3982)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/04/2024 05:09:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,520
            CDSs (total)                 :: 1,474
            Genes (coding)               :: 1,474
            CDSs (with protein)          :: 1,474
            Genes (RNA)                  :: 46
            rRNAs                        :: 1 (5S)
            complete rRNAs               :: 1 (5S)
            tRNAs                        :: 43
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3982
                     /organism="Nitrosopumilaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1632655"
                     /isolate="SMAG_U16342"
                     /isolation_source="soil"
                     /db_xref="taxon:2202732"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..188
                     /locus_tag="VLB45_00495"
     CDS             <1..188
                     /locus_tag="VLB45_00495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04217.1"
                     /translation="SHELSHEFLRQAGDKRYVELIHDIWTKHLFAALPFEHYDKDFAK
                     TEKPSVFSTIDASEFRL"
     gene            complement(606..1238)
                     /locus_tag="VLB45_00500"
     CDS             complement(606..1238)
                     /locus_tag="VLB45_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007551318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04218.1"
                     /translation="MSRSNFPSFGASKTGTYVSALKNYNLPHFILEKVALDCESDLIK
                     KGRIEDRLQSMNDSALELLHRVFVDCEEDTAGMYANYRFCAYISSMFHKCEILLNEKI
                     QGASGKAHKVPVAVKNNGMYIAIGFNKSTGGPISKKEAIKFYEIIDDIKKGEHGNLLT
                     DGIYGSSVGFEGEALVNLEKLSKLRKKDPENKIEFKTASFENRIYSVTKC"
     gene            1681..2214
                     /locus_tag="VLB45_00505"
     CDS             1681..2214
                     /locus_tag="VLB45_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04219.1"
                     /translation="MTRLLLLLLGAIPVLVAIFIVIPQITQPEIPITAVDSNDVLTVE
                     YSKQYLKRVTFGVTERLGAQETELLAIENDGKVVYSVTKNGYPEPDVKSTLEKSKLKK
                     LTALIKETGFMQIPKDSFAIKNDADEFDKYGIKVTLNGKSRNIQWAEQNATEDFIPPL
                     VIQVQLELDSIIAEIME"
     gene            2240..3208
                     /gene="pdxS"
                     /locus_tag="VLB45_00510"
     CDS             2240..3208
                     /gene="pdxS"
                     /locus_tag="VLB45_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HSD04220.1"
                     /translation="MIPLSGDIPDSKGTINEKIESYSIKRGTSTLKRGLAHMLKNGVV
                     MDVTTVEQAQIAEDAGAVSVMVLDKLPSDVRKAGGVARTASIRIIQEIMDSVTIPVMA
                     KCRIGHVYEAMVLQETNVDMIDESEVLTPADELHHIWKWDFTTPFVNGARSLGEALRR
                     IEEGAAMIRTKGEPGTGNVAEAVTHIKNVNDELRKIKSIYDSGDQQDLIRMARELKVS
                     YSLVEETASLGRLPVVNFAAGGISTPADAAYLMTLGCDGIFVGSGIFKAEDAKERAQA
                     IVLATTFWEDPDKVKEAQKMIDERQSMLGLDTKNLELRMQERGSTA"
     gene            3205..3822
                     /gene="pdxT"
                     /locus_tag="VLB45_00515"
     CDS             3205..3822
                     /gene="pdxT"
                     /locus_tag="VLB45_00515"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HSD04221.1"
                     /translation="MSGLNIGVLAVQGDVAENIMATKMALEELGIEGIVNEIKLPEQI
                     ADLDGLIIPGGESTVIGQLSLVNGSLKQIKEKISSGMPVFGICAGMILLSKKAKDRVV
                     GEMDQPLLDLLDVRVERNTFGRQRDSFESEISMDKIGIPKFHGVFIRAPSITETGKSV
                     EVISKFNEKIIAVKQGKILGTSFHPELTGDLSLHKYFINLVRENQ"
BASE COUNT         1358 a          676 c          803 g         1145 t
ORIGIN      
        1 tatcccatga actgtctcat gaatttctca gacaagctgg tgacaaaaga tacgttgaat
       61 tgattcatga tatatggacc aagcatcttt ttgcagcatt gccattcgag cattatgata
      121 aagattttgc aaagacggaa aaaccgtcgg tattttcaac aatcgatgca tcagaatttc
      181 ggttatagta aaatcaccag tctcttttaa tcttaaaatt tgtaatttag ttcatgttaa
      241 gctgtaaagg agaatgtaaa actactttca aacgttcact tggaaaaacg atctttcatt
      301 atactgatgg acaaaaaagg tgcagtgttt gtggtgtgta tattagatgg ataggatcaa
      361 aatgtccttg ttgtaatgcg gtgttgcatg taagacctag acattcgcgt gcaaaagaaa
      421 agtattatga gattgccgga atcaaatggc tgtaaaagcc tataagtagg tctgtatgga
      481 tttttttcat gcctgaaata atctgtcctg attgcggtaa gaggatgttt cacgaaaatg
      541 aaactacctt aaacattgaa aaagcagtgc acgataaatt ctgcattaaa aaggcaaaat
      601 aggccttaac actttgtaac tgaatatatt ctgttttcga agcttgctgt cttgaattct
      661 atcttgtttt ctggatcttt tttacgtaat ttgcttaatt tttcaagatt gacaagcgct
      721 tcgccttcaa aacctactga ggaaccataa ataccgtctg taaggaggtt tccatgttct
      781 ccttttttga tgtcgtcaat aatttcataa aacttgattg cttctttttt ggatattgga
      841 ccgcctgttg acttgttaaa ccctattgca atgtacatgc cgttattttt tacggcgact
      901 ggtactttgt gggctttacc tgaagcacct tggatttttt cattaagcaa aatttcgcac
      961 ttgtggaaca tgcttgagat ataggcacaa aaacgataat tggcatacat gccagcagta
     1021 tcttcttcac aatcaacaaa aactctgtga agcagttcaa gagcagagtc gttcatgctt
     1081 tgcagacgat cttcaattct gccctttttg attaaatccg attcacaatc cagagcaact
     1141 ttttcaagaa tgaaatgagg caggttgtag tttttcagtg cagaaacata agttcctgtt
     1201 tttgacgcac caaaacttgg gaaattgcta cgactcaatt cagaatttct ccaaaattat
     1261 gattaataac aaatttaaat ttcgtaacca tgcagatttt gtttaagtgg ctttttgctt
     1321 ataactgttc gctaaattat ggatcaaaat ggtggggccg accggagttg aaccggtgac
     1381 acacgggtca ctacagctcc aaactgtaca tcatcctttc gtcagtgaag cgtgttgaac
     1441 cactggagcc cttagcggtg atcatgtgat ctatcctgtc gccatgcctg gctaggctac
     1501 gaccccacgc aaggagatga atacactaga attttaattt acttaagtgt cgaagattta
     1561 tttgccttta agataattac gttaagacaa gtatgaaaaa tacatctaaa aaatttattt
     1621 taccatgtct agattcaaac aatctgttaa accataggtt acagattttt acaaattgaa
     1681 atgactcgac ttttattgct tttacttgga gccattccag ttcttgtcgc tatttttatt
     1741 gtaattccac aaataaccca acctgaaatt ccaataactg cagtggattc aaacgacgtg
     1801 ctcacagtag aatatagtaa acagtacctc aagagagtga catttggagt cacagaaaga
     1861 ttaggtgcac aagagactga gcttcttgca attgaaaatg acggtaaagt tgtgtacagc
     1921 gtaacaaaga atggatatcc agagcctgat gtaaaatcaa cactagaaaa atccaaacta
     1981 aagaaactta ctgcattgat aaaagaaaca ggttttatgc aaatcccaaa agactctttt
     2041 gcaataaaaa atgacgctga tgaatttgat aaatatggaa taaaggttac actaaacggt
     2101 aaatcgcgta atattcagtg ggcagaacaa aatgcaactg aagacttcat tcctccgctt
     2161 gttattcaag tgcaattaga acttgacagt ataattgcag aaataatgga ataaatatca
     2221 ctcgtaatca tcatcaaaca tgattccact ttcaggcgat attcccgatt caaaaggaac
     2281 aataaatgag aagatagaat cttactctat caagcgtgga acatccacac taaaacgtgg
     2341 attagctcac atgctgaaaa atggagttgt aatggatgtt acaactgttg agcaagctca
     2401 gatagctgaa gatgctgggg ctgtttctgt aatggtattg gacaaattgc catctgatgt
     2461 aagaaaagca ggtggtgttg caagaactgc aagcatcaga attattcaag aaatcatgga
     2521 ttcagtaaca attcctgtca tggcaaaatg cagaatagga catgtctatg aagcaatggt
     2581 tttgcaagaa actaatgtcg acatgattga cgaatccgaa gtacttactc cggcagatga
     2641 attacaccat atatggaaat gggattttac aacaccattt gtaaatggcg cacgaagtct
     2701 tggagaagca cttcgcagaa tagaagaggg cgcagctatg atacgaacaa aaggagaacc
     2761 aggaacagga aatgttgcag aagcagtgac ccatattaaa aatgtaaatg acgaattacg
     2821 aaaaataaaa tcaatttatg actcaggaga tcagcaggat cttatccgca tggcaagaga
     2881 actgaaggtt tcttattctc tagtagaaga aactgcgagt cttggaagac taccagttgt
     2941 taattttgcg gctggtggta tatctacgcc agcagatgct gcatatctta tgactcttgg
     3001 ttgtgatgga atttttgttg gctctggaat attcaaggca gaggatgcaa aggaaagagc
     3061 acaggcaata gtacttgcaa caactttctg ggaagatcct gacaaggtca aagaagctca
     3121 gaaaatgata gatgaaaggc aatcaatgct tgggcttgat accaaaaatc ttgaattacg
     3181 aatgcaagaa cgcggaagca cggcatgagt ggattaaaca taggagttct cgcagttcaa
     3241 ggagatgttg cagaaaacat catggcaaca aaaatggcgc tagaagagct tggaatagaa
     3301 ggaatcgtaa atgaaataaa acttcctgaa caaattgctg atcttgacgg tttgataatt
     3361 cctggagggg aaagcactgt aataggtcaa ctctcgcttg tcaacggttc actaaaacag
     3421 ataaaagaaa aaatttcttc tggcatgcca gtatttggaa tctgtgcagg catgatattg
     3481 ctctcaaaaa aggcaaaaga tagagtggtt ggtgaaatgg accaacctct gttggatctt
     3541 ttagatgtaa gagtagaaag gaataccttt ggaagacaac gtgattcatt tgaatctgaa
     3601 atatcaatgg ataagattgg gattccaaag ttccatgggg tgtttattag agctccatcg
     3661 ataacggaaa ctggaaaaag cgttgaagtg atatcaaaat tcaatgaaaa aattattgca
     3721 gtaaagcaag gaaaaatact tggaacttcg tttcatccag aattgacagg agatctgtca
     3781 ctgcacaaat acttcataaa cctagttcga gaaaatcaat aatcgtactt gacatgttaa
     3841 gaaattctgt aaactgtcta ggacgattta tattaaattc aagctctgat caatagtagt
     3901 tatgaaaata gaaagcacgc cagaacttgt gtcatctatt tacaaaaaat cagaagagaa
     3961 tataacaaaa tttagaaaaa tg
//
LOCUS       DATIDL010000011        13727 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrosopumilaceae archaeon isolate SMAG_U16342
            k141_1633032, whole genome shotgun sequence.
ACCESSION   DATIDL010000011 DATIDL010000000
VERSION     DATIDL010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935836
            Sequence Read Archive: SRR25158326
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosopumilaceae archaeon (soil metagenome)
  ORGANISM  Nitrosopumilaceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrosopumilales;
            Nitrosopumilaceae.
REFERENCE   1  (bases 1 to 13727)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13727)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/04/2024 05:09:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,520
            CDSs (total)                 :: 1,474
            Genes (coding)               :: 1,474
            CDSs (with protein)          :: 1,474
            Genes (RNA)                  :: 46
            rRNAs                        :: 1 (5S)
            complete rRNAs               :: 1 (5S)
            tRNAs                        :: 43
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13727
                     /organism="Nitrosopumilaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1633032"
                     /isolate="SMAG_U16342"
                     /isolation_source="soil"
                     /db_xref="taxon:2202732"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(154..612)
                     /locus_tag="VLB45_00520"
     CDS             complement(154..612)
                     /locus_tag="VLB45_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016939303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15e"
                     /protein_id="HSD04222.1"
                     /translation="MISHQDQVWLKMWQENSPELRAKAIEWRKQNALTRIERPSRIQK
                     ARRLGYKAKQGIVVVRMRVGKGNMRKKRPVAGRRPKHLGVTRIKPDVGMKQVAERRVL
                     ERFPNMKLLGSYFLYKDGMYLWYEVIMADPSHPRIFKDKEMRGKIAGLKN"
     gene            complement(703..1419)
                     /locus_tag="VLB45_00525"
     CDS             complement(703..1419)
                     /locus_tag="VLB45_00525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04223.1"
                     /translation="MKNLKLLMPFLALLSFSPILTYAEQQTISVDFGKLYEITYDAKD
                     VTVSEVEPNVDEIELIFHVDVTSPIATLELTIPRDLLDSKENGDDIDFFIIADGELIT
                     FSEKESTETTRTLFIQLSPGTNEVEVFGTHLAEKTFEDESAVPDEDQPQIEETESESD
                     EQPTEETVPETTEESMESPTETTEKEPEQQTTGSTILKLPAWSMDISKRQMTEFSVAA
                     GLFLFLAIVIAAVKGSRSKQ"
     gene            complement(1420..2352)
                     /locus_tag="VLB45_00530"
     CDS             complement(1420..2352)
                     /locus_tag="VLB45_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HSD04224.1"
                     /translation="MSFNQSVLICDQVAPILNEILQKNGLKITYEPEITPEQIKEKIS
                     NFNIVIVRSRTKITKEMIEKATQCKIIARVGVGLDNIDISAAKAKGIRVINAVEGAMN
                     AVAELVLGLMLSLAREIPRADREIRNGNWLKKELMGTELSGKYLGIVGLGNIGKRLAK
                     HARALNMNIIGYDVIPIPDDFSREVGLVKTDLDTLLSSADYVSFHVPFTNETHHLVNS
                     QRINKMKKTAYIINTSRGEIIDEDALYDALKNGKIAGAALDVFEKEPATGNKLASLPN
                     VICTPHIGAQTKEAQSLSANVIGEKIIMILRGVI"
     gene            complement(2425..3765)
                     /locus_tag="VLB45_00535"
     CDS             complement(2425..3765)
                     /locus_tag="VLB45_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HSD04225.1"
                     /translation="MNKRYSMLSIAAVAVIAATLFGSTYTQPQTTGSALSVSKTDADV
                     MQKIHDMGGLELIMPQAFAQVDCANMEDGRNVVEFNITGESADLPIMGGGTYKAMTFS
                     GQIPGPTLRVTQGDIVKMTLSIPEGEPTPHGNDMHASQMSAGNFGKVLPGTSKTYCYV
                     AQVPGTFKYHCSGVDVAAMDQHVLSGMYGMTIVDPLDGYKKLMVESTAVEGGDVVKDR
                     IMYPADALEFQLQYNQLYLTEDGSYDMNAMMQHHHTLAVVNGMSFGYVPNETHNELIN
                     GAKGKNIFVAQPWNSAELGQYQSQVLFVPTGVHTRWFVENQGNEPVYFHIVGEIIDRV
                     TQANRVQAQGTETWLIGGSQGAIMDVVFDNPGVYVVVNHDYAQIFSGTASVIVAGNPF
                     GLEVGDVDSLAEVLGNPSDAVPPSGMNTMEHPKQNLHGLYTDARADELKSELGI"
     gene            3907..5268
                     /locus_tag="VLB45_00540"
     CDS             3907..5268
                     /locus_tag="VLB45_00540"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HSD04226.1"
                     /translation="MKFRIDQDSLGEVKIPEDAYYGAFTARAIQQYHVTGQPSHLYLI
                     MAFVMIKRSAAVANMKTRALDKKRGNAIVKACDVILSGKFLDQFVVEAINSGAGTALN
                     MNTNEVIANVALEILGHKKGKYNIIHPNDHVNMSQSSNDTFPTAMHVSILFNLKQVIP
                     ALDELIRSLNKKAKEFSGFQKIGRTHLMDALPVSLGKEFAAYSTAIAKARDAVVAARK
                     ELEFVALGGTAVGTGANTPKGYRNIVISELGRISKLQLKPQQDMQYSLQSKFAVANLS
                     SALRNLALEIGKISNDIRLMASGPVAGLSELGIPAVHAGSSIMPGKVNPSLAECMNMI
                     CFNIIGNDTAVALAAQAGQFELNVMLPVMVKCVLESTDMLRNFVPIFSRNLINGLSAN
                     KRKLNQYIENSPVIVTLLAPKLGYQTSAELYKESLKTGKTIRQLVISKGLLSEKEVKT
                     LLG"
     gene            5270..6538
                     /locus_tag="VLB45_00545"
     CDS             5270..6538
                     /locus_tag="VLB45_00545"
                     /EC_number="2.8.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase"
                     /protein_id="HSD04227.1"
                     /translation="MAKIWVEAYGCSASFADSEMISGLIINGGHTLAQNSSDSDLNLI
                     VTCSVKDATADKMVNRIKKLKSKPLIVAGCLPKAERLTVEKFGSNASMLGPNALGKTL
                     DVINSTLKGKRKIELEDSDLIKVGLPKVRLNPVVGIIEIASGCMSECTFCQTKLSKGD
                     LHSYRLGDIVRQVKQDIKDGCKEIWLSSTDNGCYGLDIGTDLPGLVNAIGEIEQDFMI
                     RVGMMNPMYMPRIRDGLLRSFESNKVFKFLHIPVQSGSNKILKDMRRGHTAKVLRDAN
                     EKFRRKFGKFTVSTDIIVGFPSETEEDFAQTVDLIKETRPDIINLSRYSARPGTKAAK
                     MNQLEISEVKRRSKVVFELTKKIERERNEEWLGWQGEVLFDESSDGTVKGRNFAYKPI
                     VIEEPVKLGQKIKVEIIKATQHGLWGKTLS"
     gene            6613..7566
                     /locus_tag="VLB45_00550"
     CDS             6613..7566
                     /locus_tag="VLB45_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HSD04228.1"
                     /translation="MEFEMKEPILIVGVGGAGCKLAVKAKSSLNSECLLISQDVKDLE
                     GDGSKVLINTNPVLNPSAYLIRGISFGAQDILREKFSNYSTVVIIANLAGKSGAALAP
                     VVSQIGKELGKNVLSFGIMPFKFEKDRIFFSGISLKRLKVNSDCTVIVDNDALLDSNP
                     DLTPESCNMITNAALLEVVSSLKSSSLPLETNILSTSKDVSDVQTSLRDSIKMLYEDA
                     PPNSVKSAMLYVMGGNKVSVGVLNSMVNTISGIFNDDSTRVSLSVVAAERSKVILLTS
                     IDGETRFDKYDPLGIISKQNTIDWDEPDCSIKLDLDLKQLE"
     gene            complement(7567..8052)
                     /locus_tag="VLB45_00555"
     CDS             complement(7567..8052)
                     /locus_tag="VLB45_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31e"
                     /protein_id="HSD04229.1"
                     /translation="MSQEQIERVYTINLGKVLLSPNNQRAKRAINMIREFAVRHMKSE
                     DIKIEEDVSHLVWARGIRHPPRKIRVKLTKDDDGTVLISKYEEETKAKKEVVEEKPKP
                     SEKKIEEKPTVEKAEPKSENQKPEAKEVKTKTESKPEKPKKEVKSEKKSKEKSAKPKK
                     K"
     gene            complement(8054..8257)
                     /locus_tag="VLB45_00560"
     CDS             complement(8054..8257)
                     /locus_tag="VLB45_00560"
                     /inference="COORDINATES: protein motif:HMM:NF002316.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L39e"
                     /protein_id="HSD04230.1"
                     /translation="MSEIHLKRALEAFHFMAAHKSTPRKIRLMKKLKQNSSVPAWVII
                     KTKRSVRTNPKRRQWRRTDVRVG"
     gene            complement(8298..8849)
                     /locus_tag="VLB45_00565"
     CDS             complement(8298..8849)
                     /locus_tag="VLB45_00565"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HSD04231.1"
                     /translation="MKKEEIQKALSSEIMPEPKRTGNAKLAAVLVVIHGQEPTIIMTE
                     RPKTMTLHAGEISFPGGTWKQNDDDLLTTALRETREEISLGISRDQVIGQLKPVTTLN
                     SGFTITPFIAITPKITNLVPNHEIEEILNIPLFSLLKTMTHDPDPTHQSIQEMYTFTY
                     QDKLIWGASARILKQMASLLLEK"
     gene            complement(8916..11414)
                     /locus_tag="VLB45_00570"
     CDS             complement(8916..11414)
                     /locus_tag="VLB45_00570"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008301613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HSD04232.1"
                     /translation="MKIPRVSSALKAVELRQVPAPLIIGERLNTQGSKKAKESVLNNN
                     FEELLNLAKAQVEDGAHCLDVCVATTERSDELEFMKKLVKLLSLEIDAPLVIDSTESK
                     VISAALEQIPGKPIINSINLEGDGNRFHSLAPLMAKYGVPAIAMCIGPKGMAKTPQEK
                     LETAELLYETGKKYGLKEEQFIFDALTFTLATGESEFQDAGKNTIEGIRLIKQRFPNS
                     FTTLGLSNISFGLAPNARKVLNAVFLYHAVKVGLDTVIINPKDVIPYSEINEKEKKLA
                     EDLIFNSHPNALAELITYFESTKSTTVATKKTEIDPAWSAGKRSNFRIVNRIKDGIEN
                     DVVRAIAEKIPQKPQLIEKNGSLAIQAQKEITHDAAIQTLNEDLLPAMKEVGDKFGAG
                     ELILPFVLKSAECMKAAVGELEKYLLKEEGTSKGKLVLGTVYGDVHDIGKNLVKTIFE
                     NNGYTVYDLGKQVPLQKFLEKIDEVKPNAIGLSALLVSTSKQMQYFVEHARKTGMHMP
                     ILCGGAAINSNYINRISKEGGIYKSGVFYCKTMFEGLKTMDKLVSDEKEQFVQEWEKR
                     LEKWQERAVTQIEPENIPHSGIKPVVAPVPPSVNKPIRLDPVHIDLKEVWELIDKKSL
                     FVLSWGLRGESAKDSQKEHEKLFEEWKERIIKEDLFEPHAVYGYYKCHNTGGKLVVDH
                     PNGEQLVFDFPRSSKEKHLCLTDYFGENDIVAFQSVTVGNKVSEIIEKWNKEDRYTDA
                     YYLHGLAVETAEAMAEWINRKIRYELKIGEKRGLRYSWGYPSCPDVSQHRLVWKLLEP
                     EKSGMTLTDAGQIVPEQSTAAIVVHHPEAEYFTI"
     gene            complement(11461..12405)
                     /locus_tag="VLB45_00575"
     CDS             complement(11461..12405)
                     /locus_tag="VLB45_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HSD04233.1"
                     /translation="MNKDHFLDALKNKILLFDGAMGTEIQKLNPKPEDFPDGKDGFND
                     GLSFTKPEWIKKIHRNYLEAGADCIETNTFGSNKLKLDEYGYGDQTVEFNKKIAQLAV
                     DVCKEFTDKPRYVIGSMGPTGYLPSSNDPSLGQIPLEEIKNAYELQAEGLILGGVDAL
                     LIETSQDILEVKLAIEASHIAMKKTGKTVPIIANVTLDQYGKMLLGTNVQAAYTAVSD
                     MGIDAFGLNCSTGPMEMTSSVQWLNEQNDLPLLVVPNAGMPENQGGKAVYKMTPEDMA
                     KALKDFVMAYPKVRIIGGCCGTNPSHISLLRKVIDQKV"
     gene            12505..13260
                     /locus_tag="VLB45_00580"
     CDS             12505..13260
                     /locus_tag="VLB45_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5,10-methenyltetrahydrofolate synthetase"
                     /protein_id="HSD04234.1"
                     /translation="MTITYELNPPKVIQDTVLSHNELQDSVNKLKERSLKIGKVCNSI
                     HVTDSVLGIPRISPITVGTFIRNSNKKIKISASIRVRDRNLTAVTQTICDAILLDLNG
                     VLVLKGDKPPAGPQDSKLVPSEVVKQFNEQGFGKKIDLYLSLPSNPDFDKIHKKIEAQ
                     PKGFVTQVISSVDQVTRIVDKLKPQGFKIIPCVLLSSEKNSKSAKMLNLDWSSYSNDV
                     VGFINKVNKIAENVLVSSPSDFSGALEVLKKLV"
     gene            13323..>13727
                     /locus_tag="VLB45_00585"
     CDS             13323..>13727
                     /locus_tag="VLB45_00585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HSD04235.1"
                     /translation="MKYSVIIQASFVVFVLVLMVQYANAEVWIPENEFSSYFDEDGIY
                     TVIGAVKNSESYPIVPTIELRIQDGNKIISESYTLPVANPLKDIPFKIKFDQISSKNP
                     TLEEPAVTFVPGPTDYMNIEIVYDDTLVRHKDG"
BASE COUNT         3976 a         2655 c         2546 g         4550 t
ORIGIN      
        1 tttcaaaatt tgctactgtt tccacttcaa aatcataccc accagtttca ataacaaaat
       61 cctttttcag acctgtttta tctgaaatgg ctccaaatgc tggtggcaaa aacattataa
      121 ctatagttac gaaaattaaa aaatttagta tcttcaattt ttaagaccgg caatctttcc
      181 acgcatctct ttatctttaa agattcttgg atgtgatgga tctgccataa ttacttcata
      241 ccacaagtac attccgtcct tatagagaaa gtacgaaccg agaagtttca tgttaggaaa
      301 tctttctaac actctgcgct ctgctacttg tttcattcca acatctggtt ttattcttgt
      361 gacaccaaga tgtttaggtc ttcttccagc aacaggtctt tttttacgca tgttgccttt
      421 tccaacacgc attcgcacta ctacgatacc ctgttttgct ttgtatccta gtctcctagc
      481 cttttggatt ctgcttggcc tttctattct tgtaagagca ttttgctttc tccactctat
      541 tgcttttgct cttaactctg gcgaattttc ttgccacatt ttaagccaaa cttgatcttg
      601 atggctaatc atattcggca ctaccaaatt ccccttaata tctgtaccag acgttctttt
      661 gaaaatctaa tttagagaaa gaggagatat gatgtaacaa atctattgtt tagatcttga
      721 tccttttaca gccgcaatta caatggcaag aaacaagaac agccctgcag ctactgaaaa
      781 ttcggtcatc tgtcttttgc ttatgtccat tgaccatgct ggaagtttta gaatagtgga
      841 gccagtagtt tgttgttcag gttctttttc tgtagtttca gtaggacttt ccatggattc
      901 ttcagttgtt tctggcacag tttcttctgt aggctgttca tcggattcac tctctgtttc
      961 ttctatctgt ggttggtctt catcaggtac ggcgctttca tcttcaaaag ttttttctgc
     1021 cagatgagtt ccaaacacct ctacttcgtt tgtaccagga gatagttgaa tgaagagagt
     1081 cctagtggtt tctgtggatt ctttttctga aaatgtaata agctcaccgt cagcaattat
     1141 gaagaaatcg atgtcgtcac cattttcttt agaatcaagc aagtctcttg gaattgttaa
     1201 ctctaatgtt gcaatgggac ttgtaacatc cacatggaaa ataagctcaa tctcatcaac
     1261 gttaggctct acctcagaaa cagttacatc ttttgcatca taagtaattt catataattt
     1321 tccaaaatct acagatattg tctgttgttc agcatatgtc agaatcggag agaaactaag
     1381 taaggcaaga aatggcatca ataattttaa atttttcaat cagattacac cacgcaagat
     1441 cattattatt ttttctccta taacatttgc agataagctt tgggcctctt ttgtctgagc
     1501 acctatatgc ggagtacaga tgacatttgg aagtgatgca agtttgttac cagtagcagg
     1561 ttctttttca aacacgtcta atgcagctcc tgcaattttg ccatttttta gtgcatcata
     1621 aagagcgtct tcatctatta tttctcctct tgaagtattg attatgtaag cagttttttt
     1681 cattttgttg attctttgtg aatttacaag atgatgcgtc tcatttgtaa acggcacatg
     1741 aaatgacaca tagtctgcac ttgaaagcaa agtatccaga tcagtcttta caagacctac
     1801 ttctcgtgaa aaatcatctg gaattggaat tacatcatat ccaataatgt tcatgttaag
     1861 agcccttgca tgtttggcca atcgtttccc gatgttgcct aaaccaacaa ttcctaaata
     1921 tttaccagag agctctgttc ccatcaattc ttttttcaac cagtttccat tgcgtatttc
     1981 cctgtctgct cttgggattt cgcgtgcaag cgacagcatc aaacctaaaa ctaactccgc
     2041 tacagcattc attgcgcctt caactgcatt gatcactcgg attccttttg cttttgcggc
     2101 actaatgtca atattgtcaa gaccaacacc caccctagct attattttac actgtgtagc
     2161 cttttcgatc atttcttttg tgatcttggt cctgcttcta actattacaa tattgaaatt
     2221 tgaaattttt tcttttattt gttcaggagt aatttcaggc tcgtatgtta tttttaatcc
     2281 atttttttgc agtatctcgt ttagaatggg tgcaacttga tcacagatca gaaccgattg
     2341 attaaaactc atgagaatga accactatgc acagaatata atcattgtgc aacataaaaa
     2401 aacaaaaaag ggaatattag ggatttagat tcctaattca gattttaatt cgtcagcacg
     2461 tgcatcagtg tatagaccat gcaagttctg cttcgggtgt tccattgtgt tcattcctga
     2521 tggaggaact gcatcgcttg gattgcccaa aacttcagct aatgaatcta cgtcgccaac
     2581 ctctagtccg aatggatttc ctgcaacaat tactgatgcg gttccgctaa agatttgtgc
     2641 atagtcatgg tttactacta cgtaaactcc tgggttgtca aagacaacat ccattattgc
     2701 tccttgtgat ccgccaatta accatgtctc tgttccttgt gcttgtaccc tgtttgcttg
     2761 ggtaactctg tcaattattt ctcccacaat gtggaagtaa actggctcat tgccctggtt
     2821 ttcaacaaac catcttgtgt gaacaccagt tggtacaaat aggacttgag attggtattg
     2881 tcctaattct gcagagttcc acggctgtgc aacaaagatg tttttacctt ttgcaccgtt
     2941 tatcaattcg ttatgagtct cgtttggaac ataaccaaat gacattccgt tgactactgc
     3001 tagagtgtgg tgatgctgca tcatggcatt catgtcgtag cttccatcct cagtcaagta
     3061 caattgattg tactgtagct ggaattctaa agcatcggca ggatacatta tcctgtcttt
     3121 tactacatcg ccgccttcaa ccgcggttga ttctaccatg agtttcttgt atccatcgag
     3181 tgggtcaacg attgtcatac cgtacatgcc ggataagacg tgttgatcca ttgctgctac
     3241 atcgacacca gagcagtggt acttgaaagt accaggtact tgtgcaacat agcagtatgt
     3301 cttggatgtt cctggtagaa ctttaccaaa gtttcctgca gacatctgtg atgcatgcat
     3361 atcgttaccg tgtggagttg gttctccttc tggaattgaa agtgtcatct taacaatgtc
     3421 accttgtgtg actctgagtg ttggtcctgg tatctgacca ctgaaggtca ttgccttgta
     3481 tgtacctcca cccataattg gcaagtctgc actttctcca gttatgttga attcaactac
     3541 atttcggcca tcttccatgt tagcacagtc gacttgagca aatgcttgtg gcattattag
     3601 ctctagtccg cccatgtcat ggattttctg cattacatct gcatctgtct ttgaaacaga
     3661 cagtgcactt ccagtcgttt gtggctgggt atacgtgctt ccgaacaacg ttgcggctat
     3721 cactgctact gcagcgatag atagcatact gtaacgcttg ttcatcgact cggatcgatt
     3781 attttcagta tattaatcta ttgtaggaaa ttctgaaata tagttgaaaa aataaaaaat
     3841 ttaagattaa attttatatt tttttaaaag cgaatgaata ttaggaaaat aaagtctagc
     3901 ttgcatgtga agtttagaat agatcaagat tctttgggtg aagtaaaaat tccagaagat
     3961 gcgtactatg gagcatttac tgcaagagca attcagcaat atcacgtaac aggccaacca
     4021 agtcacctct atctaatcat ggcatttgtc atgatcaaga gatctgctgc agttgcaaac
     4081 atgaagactc gcgcacttga taaaaaacga ggaaatgcaa ttgttaaggc gtgtgatgtt
     4141 attttgtcag gcaagtttct tgatcagttt gttgtagaag caataaactc tggagcagga
     4201 accgcattga atatgaatac aaatgaggta attgcaaacg tagctttaga aattttagga
     4261 cataaaaaag gaaaatacaa cataattcat ccaaatgatc atgtcaacat gtcacagtca
     4321 agcaacgata cttttccaac tgcaatgcat gtttccattt tgtttaatct caaacaagtc
     4381 attccagcac ttgatgaatt gataagatca ttaaataaaa aagctaaaga gttttcaggt
     4441 tttcagaaaa ttggaagaac ccatttgatg gatgcacttc cagtaagtct tggaaaagaa
     4501 tttgcagcgt attctacagc tatcgcaaaa gctcgggacg cagtagtcgc tgcaagaaag
     4561 gaactagaat ttgttgcact gggtggcact gctgtaggta caggagcaaa tacaccaaaa
     4621 gggtatagaa atattgtaat atcagaactt ggtcgaatct caaaacttca gctcaaaccc
     4681 caacaagaca tgcagtattc tttacaaagc aagtttgcgg tagcaaacct atcttctgca
     4741 cttagaaatc tggcactaga gataggaaag atatcaaacg atattagact gatggcatcc
     4801 ggtcccgtag caggactttc agaacttggc atacctgctg tccatgcagg ctcatcaatt
     4861 atgccaggta aagtgaatcc atcacttgca gagtgcatga acatgatttg ttttaatata
     4921 attggaaatg acacagcagt agcacttgca gcccaagcag gacagtttga acttaatgtt
     4981 atgctccctg taatggtaaa gtgtgtttta gaatcaacag atatgttgag aaactttgtt
     5041 ccaatatttt ctcgtaattt gataaatggt ttgtctgcaa ataaacgaaa gttaaatcaa
     5101 tacatagaaa atagtcccgt aatagttact ttgctagctc caaagcttgg atatcaaaca
     5161 tctgccgaat tatacaaaga atccctgaaa acaggtaaga cgatcagaca gcttgtcatt
     5221 tcaaaaggac tcttgagtga aaaagaagta aagacgttgt tgggataaga tggcaaagat
     5281 ctgggttgaa gcttacgggt gctctgcaag ctttgcagat tctgaaatga tttcaggttt
     5341 aattataaat ggcggtcata cacttgcaca aaactcatca gattctgatt taaatctcat
     5401 tgtaacctgt tctgtcaagg atgcaactgc agacaagatg gtaaatagaa taaaaaaact
     5461 aaaatccaaa ccacttattg ttgcaggttg tctgccaaaa gctgagagac ttacggttga
     5521 aaaattcggt tctaacgcaa gcatgttagg accaaatgcg ttgggaaaaa ctcttgatgt
     5581 cataaattcc acattaaagg gaaaaagaaa aatcgagttg gaagattctg acttgatcaa
     5641 agtaggatta ccaaaagtca gattgaaccc agttgtaggc ataattgaga tcgcaagcgg
     5701 atgtatgagc gaatgtactt tttgccagac taaactttcc aagggagact tgcatagcta
     5761 tagacttggg gacatagtgc gtcaggtgaa gcaagacatt aaagatgggt gtaaagagat
     5821 ttggctttcg tctacggata acggctgtta tgggttagac ataggaacag acctaccagg
     5881 actagtaaat gccataggcg agattgaaca agattttatg attcgtgtag ggatgatgaa
     5941 tcccatgtac atgcccagaa ttcgtgacgg attgttacgg tcttttgaaa gcaacaaagt
     6001 cttcaaattc cttcacatac ctgttcagag cggaagcaac aaaatactaa aagacatgag
     6061 acgtggtcat actgccaaag ttttgagaga cgccaatgag aaattcagaa gaaaatttgg
     6121 aaaatttaca gtttctactg atattatagt aggttttcct tcagaaacgg aagaagattt
     6181 tgcacagacc gtggatttga taaaagagac aagacctgac atcataaacc tctcaagata
     6241 tagtgcaagg cctggtacta aagctgctaa aatgaaccaa cttgagatat cagaagtaaa
     6301 acgtagaagc aaggttgtct ttgaattgac aaaaaagata gaacgagaga gaaatgaaga
     6361 atggcttgga tggcaaggcg aagttctgtt cgatgagagc tctgacggca cagtcaaagg
     6421 cagaaatttt gcctacaaac cgatagtcat tgaggagcca gtaaaactag gacaaaagat
     6481 caaagttgag atcatcaaag ctactcaaca tggactttgg ggcaaaactt taagctaaaa
     6541 gtttttagat ccaagctggg atcaaaacta atcaacaaag ttttttaacc tatacgggaa
     6601 gcaaaaggag ttatggagtt tgaaatgaaa gaaccaattc ttattgtcgg tgttggaggc
     6661 gcaggatgca aattagcagt aaaagccaaa tcaagtctaa attcagaatg ccttttgatt
     6721 agtcaggatg tcaaggactt ggagggagat ggttctaaag tcctcataaa tacgaatcct
     6781 gtccttaacc catcagcata tctcataaga ggaatttcat ttggtgcaca agatatcctt
     6841 agagagaaat tttccaatta ttccacagta gtaataatag caaatttggc aggcaagtca
     6901 ggcgcagcct tggctccggt tgtttcccag ataggcaaag agcttggaaa aaacgtactt
     6961 tcgtttggaa tcatgccatt caagtttgaa aaagacagaa ttttcttttc tggtatttct
     7021 ctaaagaggc taaaggtaaa ttccgactgt acggtaatag tagataatga cgcattactt
     7081 gatagcaatc cagaccttac tccagagtca tgtaatatga taacaaatgc agcgcttctc
     7141 gaggttgttt cttcgttaaa atcttcttca ctgccactgg agacaaacat actctctact
     7201 agtaaggacg tatcagacgt gcaaacatca cttagagatt caataaaaat gctgtatgaa
     7261 gatgcaccac caaacagtgt caagagcgca atgttgtatg taatgggcgg aaacaaagtt
     7321 tcagtaggag ttttgaactc catggtaaac accatcagcg gaattttcaa tgatgacagc
     7381 acacgtgtta gtctttctgt ggtagctgct gaaagatcca aagtaatttt gttgacatca
     7441 atagacggag aaacaagatt tgacaagtat gatccactag gaataatttc aaaacaaaat
     7501 actatagatt gggacgaacc tgattgtagt ataaaattag atcttgactt gaagcagtta
     7561 gaataattat ttcttctttg gttttgctga tttttcttta gatttctttt cagattttac
     7621 ttctttcttt ggtttctcag gttttgattc tgtttttgtt tttacttctt ttgcttctgg
     7681 tttttggttt tcagattttg gctctgcctt ttctacagta ggcttttcct ctattttctt
     7741 ctcacttggt tttggttttt cttcgacaac ttctttcttg gctttcgttt cttcttcata
     7801 ttttgaaatc aaaacagtac catcgtcatc tttagtcagt tttactctaa tctttcttgg
     7861 aggatgtctt attcctctag cccacaccaa gtggctaaca tcttcttcaa tctttatgtc
     7921 ttcagacttc atgtgtctta ctgcaaattc tcttatcatg ttaattgcac gttttgctct
     7981 ttgattattt ggagaaagca aaacctttcc aaggtttatt gtataaacac gctcaatttg
     8041 ttcttgggac aatttatcca accctcacgt ctgttcttct ccactgtctc ctctttggat
     8101 ttgttctaac tgatcttttt gtcttgatta taacccatgc tgggacagaa gaattctgct
     8161 tcagcttttt catcagtcta atctttctag gagttgactt gtgggcagcc ataaaatgga
     8221 aggcttctaa agctcttttt aaatgaatct cagacatgca aatcgttaca cgtcaaacac
     8281 ttggatacac taaacaatta tttttccaat aaaagagatg ccatttgttt tagtattctt
     8341 gcagacgcac cccagatgag tttgtcttgg tatgtgaaag tgtacatctc ttgtatggat
     8401 tgatgtgtag gatctggatc atgagtcatg gttttgagaa gtgaaaacaa tggaatgtta
     8461 agaatctcct cgatctcatg attcggtaca agatttgtta tttttggagt aatggcaata
     8521 aagggagtta ttgtaaaacc agaattcaga gttgtaacag gtttaagctg tcctattact
     8581 tgatctctag atatgccaag agaaatctct tctctggttt ctcgtaaagc tgttgttagt
     8641 agatcgtcat cgttttgttt ccatgttcca ccaggaaaag agatctcacc agcgtgcaga
     8701 gtcattgttt ttggtctttc tgtcatgatt attgttggtt cttgtccgtg aattactact
     8761 agtactgcag caagttttgc atttccagtt cgttttggtt ctggcatgat ttcactggat
     8821 agtgcttttt gaatctcttc ttttttcaat ttacaagata ttatttgttg aaatggttat
     8881 tgattgttat cgttatttct ataatgagac actagtcaaa ttgtaaaata ttccgcttcg
     8941 gggtgatgta caactattgc tgccgtagat tgttctggaa caatttgacc agcatccgtt
     9001 aaggtcatgc ctgatttttc tggctctagc aatttccata ccaaacgatg ttgagaaaca
     9061 tctggacagc tgggataccc ccagctgtat cgtaatcctc ttttttctcc aatttttaat
     9121 tcgtaccgta ttttccgatt aatccattca gccattgctt cagcagtctc tacagcaagt
     9181 ccatgcaaat aatacgcgtc agtgtatcta tcctctttat tccatttttc tatgatttca
     9241 gataccttgt ttccaacagt aactgactgg aatgcaacta tgtcattttc tccaaaatag
     9301 tccgtaagac acagatgttt ttcttttgaa gatcttggaa aatcaaacac tagttgttcc
     9361 ccattaggat gatcaactac cagctttcca cccgtgttat gacacttgta gtagccgtat
     9421 actgcatgtg gttcaaaaag atcttctttg attattcgct ctttccattc ttcaaataat
     9481 ttttcgtgtt ctttctgaga atctttagct gactctccac gcaatcccca agataaaaca
     9541 aaaagcgatt ttttatcaat cagttcccat acttctttaa gatcaatgtg tacaggatcc
     9601 agtctaattg gtttgttaac actgggtggg acaggagcta caacaggttt tattccgcta
     9661 tgaggaatgt tttctggttc gatttgggtt acagctcttt cctgccattt ttccaacctt
     9721 ttttcccatt cttgaacaaa ctgttctttt tcatctgaaa caagtttgtc catggttttg
     9781 agtccctcaa acatcgtctt gcagtaaaac acacctgact tgtatattcc accttctttt
     9841 gaaattctgt tgatgtaatt actgttgatg gcagcaccac cacagagtat tggcatatgc
     9901 atgccagttt ttctggcatg ctctacaaaa tactgcatct gttttgatgt agatactaga
     9961 agtgcagaca gtccaatggc attgggtttt acttcatcaa ttttttccaa gaatttttgc
    10021 agtgggactt gctttcccaa gtcatataca gtgtaaccgt tattttcaaa aatagttttt
    10081 accagattct ttcctatatc gtgaacatca ccgtatactg ttcccaaaac aagtttacct
    10141 ttgcttgtcc cttcttcttt tagaagatat ttttcaagct cgcctactgc agccttcatg
    10201 cattctgcgg attttagcac aaaaggcaag attagttctc ctgctccaaa cttgtctccc
    10261 acttccttca ttgcaggaag caggtcttcg ttgagtgttt ggattgctgc atcatgagta
    10321 atttcttttt gggcttggat tgccaaagag ccattttttt ctatcaattg cggtttctgt
    10381 ggaatctttt cagcaatagc tctaacaaca tcattttcaa ttccatcctt tattctgttg
    10441 acaatcctaa agttagatct ttttccagca ctccaagcag gatctatttc tgtttttttt
    10501 gtagcaacgg tagtgctctt tgtgctttca aaatatgtta ttagttctgc aagtgcgttt
    10561 ggatggctgt taaaaattaa atcttctgca agttttttct ctttttcatt aatttcagaa
    10621 tacggaatca catcctttgg atttattata acagtatcaa gcccaacttt tactgcatga
    10681 tacagaaaca ctgcgtttag caccttacgt gcattaggtg ccaatccgaa gcttatgttg
    10741 cttaatccaa gagtagtaaa ggagtttgga aatctttgtt tgattaaacg tattccttct
    10801 atagtatttt ttccagcatc ttggaattct gattctcctg ttgcaagggt aaaggtcagt
    10861 gcatcaaaga tgaattgttc ttctttaagt ccatattttt ttcctgtttc ataaagtaat
    10921 tcagcagtct cgagtttttc ttgaggggtc tttgccattc cttttggacc aatacacatt
    10981 gcgattgcag gaacaccgta ttttgccatc aatggagcca atgaatgaaa tcgatttcca
    11041 tcaccttcca aatttatgga atttattatt ggttttccag gaatttgttc aagtgcggca
    11101 gatattactt tagattcagt agaatcaatt acaagaggag catcaatttc caagctcaac
    11161 agcttgacaa gttttttcat aaattctagt tcgtcagagc gttctgttgt tgccacacac
    11221 acatcaagac aatgtgcacc atcttcgact tgggccttgg caagatttag cagctcttca
    11281 aaattattat tcagtacaga ttcttttgct ttctttgaac cctgagtgtt taatcgttct
    11341 ccgattataa gcggggctgg gacttgacgc agctcaactg cttttagtgc agaactcact
    11401 cgtggaattt tcaacaactt gatttggagt tattttttct aaatacttag cgttgtcttc
    11461 tcagactttt tgatcaataa cttttctgag aagcgagata tgtgatgggt ttgtaccaca
    11521 acatcctcca attattctaa ccttagggta tgccataaca aagtccttga gagcttttgc
    11581 catgtcttct ggtgtcattt tgtatacagc tttgcctccc tgattttcag gcattcctgc
    11641 atttggcaca acaagcaatg gtagatcatt ttgctcattt agccactgta cacttgatgt
    11701 catctccatt gggccagtag aacagtttaa tccaaatgca tctattccca tgtcagatac
    11761 tgcagtatat gcagcttgaa catttgttcc aagcaacatt ttaccatact gatctagggt
    11821 cacatttgca ataattggca ccgtcttgcc tgtttttttc atagctatgt gtgaagcttc
    11881 gatggcaagt ttgacttcta aaatgtcttg gcttgtttca atcagcaatg catcaacgcc
    11941 tccaagtatc agaccttctg cctgtaattc atatgcattt tttatttctt caagaggaat
    12001 ttgtccaagc gagggatcgt ttgagcttgg caagtatcca gtaggaccca tactgccaat
    12061 aacatatctt ggtttgtctg taaattcttt gcaaacatca actgcaagct gagcaatttt
    12121 cttgttaaac tctacagttt gatctccata tccatactca tccagtttca atttgtttga
    12181 accaaacgtg ttggtttcta tgcaatcagc accagcttct aaatagttac gatgaatttt
    12241 ctttatccat tcaggtttag taaaagaaag accgtcgttg aatccatctt ttccgtcggg
    12301 aaaatcttct ggctttgggt tcaatttttg aatctctgta cccattgctc catcaaaaag
    12361 caatatttta ttttttagtg catcaaggaa atgatcttta ttcaagacag attttgactg
    12421 gttttaattg acttaatttc ttttgctttg agaatcattt gttcgtattc aaaggttata
    12481 atcaggtaat ctagaaaatc tcctatgaca atcacatatg aactgaatcc gcccaaggtc
    12541 atacaggata cagtactatc tcataatgag ctgcaggact ctgtaaacaa gctaaaggaa
    12601 agatctttga agattggcaa ggtctgcaat agcattcatg tgactgattc tgtgcttggc
    12661 ataccaagga tttctcctat tacagttggg acttttatta gaaattccaa caaaaaaatc
    12721 aaaatttctg ccagcatacg cgtaagagac agaaatctca ctgccgttac tcaaactatt
    12781 tgtgatgcta ttttgcttga tctaaatggc gtccttgttt taaaaggtga caagccacca
    12841 gctggaccac aagattccaa gcttgtacca agtgaagttg taaaacaatt caatgaacaa
    12901 ggatttggga aaaaaattga tctttatctt tcacttccca gcaatccaga ttttgataaa
    12961 attcacaaaa aaatcgaggc gcaaccaaaa ggatttgtaa ctcaggttat cagttcagta
    13021 gatcaggtaa caagaatagt ggacaaatta aaaccacagg gattcaaaat aattccatgt
    13081 gttttattgt cttcagaaaa gaattcaaaa tctgcaaaga tgttaaatct tgattggtca
    13141 agttatagca atgatgttgt tggcttcata aacaaagtaa ataagattgc ggaaaatgtt
    13201 cttgttagtt cacctagtga ctttagcggt gcgctagaag tattaaaaaa actagtttga
    13261 tttcagtata gaaaacgtcc tctacattaa aattcggcaa actgttctta cctgttgtct
    13321 tcatgaaata cagtgtcatc atacaagcaa gttttgttgt ttttgtatta gttttgatgg
    13381 tacagtatgc taatgcagaa gtctggattc ctgaaaatga atttagttcg tattttgacg
    13441 aggatggcat ttatacggtt attggggctg taaaaaattc agagagctac ccaatagttc
    13501 caaccataga actcaggatt caagacggaa acaaaatcat atcagagtca tacacattac
    13561 cagttgcaaa cccactcaaa gatattccat ttaagatcaa atttgatcaa atctcaagca
    13621 aaaatccaac ccttgaagaa ccagcagtca catttgttcc aggtcctaca gattacatga
    13681 atatagaaat agtttatgac gacactcttg tcagacataa agatggt
//
LOCUS       DATIDL010000012        11703 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrosopumilaceae archaeon isolate SMAG_U16342
            k141_2039526, whole genome shotgun sequence.
ACCESSION   DATIDL010000012 DATIDL010000000
VERSION     DATIDL010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935836
            Sequence Read Archive: SRR25158326
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosopumilaceae archaeon (soil metagenome)
  ORGANISM  Nitrosopumilaceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrosopumilales;
            Nitrosopumilaceae.
REFERENCE   1  (bases 1 to 11703)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11703)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/04/2024 05:09:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,520
            CDSs (total)                 :: 1,474
            Genes (coding)               :: 1,474
            CDSs (with protein)          :: 1,474
            Genes (RNA)                  :: 46
            rRNAs                        :: 1 (5S)
            complete rRNAs               :: 1 (5S)
            tRNAs                        :: 43
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11703
                     /organism="Nitrosopumilaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2039526"
                     /isolate="SMAG_U16342"
                     /isolation_source="soil"
                     /db_xref="taxon:2202732"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(144..737)
                     /locus_tag="VLB45_00590"
     CDS             complement(144..737)
                     /locus_tag="VLB45_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HSD04236.1"
                     /translation="MKLIDARKDYYRRLAHQEGYRSRAAYKLKELNRSYRIIGPGFFV
                     LDLGCSPGGWTQVAVELAGNKGKVMGIDTSYVEEIPNAHIVRGNIEDESIVELVFSHF
                     GRKVNAVICDLSPQVSGVWAVDHAKQVALNYSAAKIIEHVLAYKGNALFKVFDGEYSN
                     EFRDYIRKKFTKVQLRKPKASRKTSSELYFICLGYSP"
     gene            complement(734..1372)
                     /locus_tag="VLB45_00595"
     CDS             complement(734..1372)
                     /locus_tag="VLB45_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007551239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HSD04237.1"
                     /translation="MAKKKDKKPIKKDEVKKLVEKKKTPEKKPILVKKLVSEKKSTAQ
                     KKPAPEKKSTAQKKPVPEKKLVPKKKVEEELEEVISDEEIENFKIEGLDMEKLTNRVC
                     NIVAERGGEGMLQSELWKKLKLTSRDGSRFALKLEKRNIIKREKVLDNGRWTYKLKIS
                     RLPVGTATIEAAPCLICPVESKCSLEGEVSPRTCPLIEQWVITEFANKKTKK"
     gene            complement(1438..1911)
                     /locus_tag="VLB45_00600"
     CDS             complement(1438..1911)
                     /locus_tag="VLB45_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04238.1"
                     /translation="MAVQNTALLVFLFPVVISAAFGTFVLSGVLTEPNRELNMWPFEI
                     ATKPPVGNEDIQIQNLLKQYSTSTPVEIKVLVSNLDFDCGDLYITIYDTVAPKDVIAQ
                     NGFFNQCFSEGNVLLPIGDTFSEKVGKSGEYEIVVEMNDKDYEKTVSATAKFRVQ"
     gene            2025..4100
                     /locus_tag="VLB45_00605"
     CDS             2025..4100
                     /locus_tag="VLB45_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HSD04239.1"
                     /translation="MRLRKFRVRAFRCIHDSGDIRVGDLAAFIGRNESGKTTILEALT
                     LLNKDAKASELDLCDEMSDELKSEIKLVEGDFDLSETETKLIQDKFPNLSDIKRIKIF
                     RTQTNPKIQYDFGDVKISGEANKRINSWENFVEKIHNFVSTIPNPIRIKLDTKFLEGH
                     APRTRDAFNNMMAEFNNQIYLIASEERQVISSWEEIYRDLGNTYDSLLIGTNERSALE
                     NFVEEKLHPRFVYFSDYKKILGNIDLDEFLKETKGIRPKGLEFIEEFDKTETVKNLFY
                     LAELDPDKLEEVQNTPSRLIKLLHTSSRKLTERLNPAWKGDPIHVELRFNPGNILSVV
                     ISDVHRDGTVTNTGLLNRRAEGFKWTFSFIVNFAAETQRSELKEAILLLDEPARNLHP
                     TQQRGISDLLKGLAGSNQILYATHSPFMIFDYTPGNLLVVELDKRKHLSHIYYDYWNA
                     DDATLVPILYGLSRGLVESIPDREIGSNSRPVIIVETMVDCMYLNAFDKFLKDPNLSM
                     NPLNIVPAYNKNSVLPLAIFYRNHGYNTFVLLDNTELSRQISTMLQSNAFKPVQIIFF
                     ELGGQAKQAIEDLIVDEDYLYAVNQTYEIKLRKEGYHNLTKEDVVSKGRKSILDNLSD
                     IWRENQHLGWEKFEPEEICRYICQKIALGETDFLSDKTKDQFRAIYRLIAERIRQNQN
                     IVSETINTK"
     gene            4217..6352
                     /locus_tag="VLB45_00610"
     CDS             4217..6352
                     /locus_tag="VLB45_00610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04240.1"
                     /translation="MAATLYKGILVAILLVCSTFSVLTTPEVRGADELVTVDATNHGD
                     VTVVEYKNSQENIFDVKSVVLEIDKGGNFKSFKTEKGWVGKRTSDDTITFTSTIPIKP
                     GQSAKFGIKTDKTEPVFKWKAYDEEGDELGSGGEITTSQPKENTSGGNTQNGSSGVLD
                     GATFRLIPSTPKVDTSLRIIGEGFAPSEKLDFYIDNNRIDSFVSDENGNFILSTKIPE
                     NQKADRADFIIKDQAGNQKSMSLRIEESSKRVGIQDIELTITGDKVFYRGEEKTISGT
                     AEPGATLTISISDSSGQVLTSFTEKADSLGKYAIKHRVPADRAFGEYTITITDGKSTV
                     SHTYAVETTQKIILATTKQRYDPGETIVINGTALPDQEIEIIIEDPVGMEAYSKNMVV
                     GSNGIVNLEYPLEVSAREGTYVIFATQGDQHETFVVGVGEIPEIQLIARMSAINYKTT
                     EQAVIFITGPPSSTVSLVLVDPSDKQKFADTITVGADGYAEYSFGLTSYSPGVYTAVL
                     SRGNAQVEEKFSVGLQTGSGPITLRTVRDTFSAGETILIIGESNPNIIITVTFKDPNG
                     TKIKSADLFTDKKGTFTSNAFKFPSDAKPGIWVLDAVSGLNHVTKEITLTTGTDGELA
                     VWVDRSPPTYDNDQIVTITGSGAGQSHNIIIDIFGADQSKVESLSIVATGTGGFSTLW
                     KVPISLNAGTYTIKVIDATKTAEITITVQ"
     gene            6403..6915
                     /locus_tag="VLB45_00615"
     CDS             6403..6915
                     /locus_tag="VLB45_00615"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HSD04241.1"
                     /translation="MNLKEKISEFPDFPKPGILFRDISPLLKDPKAISFIADEFSKIY
                     KSNDVDVLAGIESRGFPLACALALKYKKGMIMIRKQGKLPGNTEKMSYSIEYGNATME
                     IQSDAVKNGQKVLVCDDLLATGGTAKTAAELIEKIGGKVTGFAFIVELTDLKGSDLIK
                     KYRHKSLVKY"
     gene            6915..7706
                     /locus_tag="VLB45_00620"
     CDS             6915..7706
                     /locus_tag="VLB45_00620"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HSD04242.1"
                     /translation="MEQAEIGIFGGTGIYDSGLLTESKEITIDTPFGKTSDSITVGMF
                     KGKKVAFMPRHGKKHTIPPHLINFRANIWAFKEMGIKRIIAPSAVGSLKEKLKPGDFA
                     LPTQFIDFTKSRKYSFFEGDKVIHISVADPFCPELQSAVSKAADKMNVKMHKNCTYVC
                     IEGPRFSTRAESKFYKEVIGADIIGMTLVPECQLAREAQMCYVSVSTVTDYDVWAEKP
                     VTAKEVIATLTKNVETTKKLLTLLPDLIPQHRRCTCEKALTEAQF"
     gene            complement(7703..7987)
                     /locus_tag="VLB45_00625"
     CDS             complement(7703..7987)
                     /locus_tag="VLB45_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010191617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04243.1"
                     /translation="MELSVHVNNVHGSQIAAKITGVFTLDGKSFRFNAIAFGRIGGHN
                     IGAKISKTTEKTLKKLGYDANEVIDVLQHKLVHGDITIPEGIKKEMFADN"
     gene            complement(7992..8390)
                     /locus_tag="VLB45_00630"
     CDS             complement(7992..8390)
                     /locus_tag="VLB45_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008301529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04244.1"
                     /translation="MAVDQDSCNHQVVYFGVTNINYEKRTIGSVDIWRCVLCKKHFCE
                     EKQLGIETLVDEVGMPKINSDQKWSVIVCKLKKHKEMWKLVKLKKDDQIQHECADEKI
                     IPLKVNDFKVQDEKHWNFLIEDSVNKIVEI"
     gene            complement(8412..8765)
                     /locus_tag="VLB45_00635"
     CDS             complement(8412..8765)
                     /locus_tag="VLB45_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04245.1"
                     /translation="MNLDIVEERLVSEVKLSPLQAKVFVLVIKKGKMTAEDIAQSLRI
                     SQEDALETAKKLIDLGGFIDITKTEFESMHPRFAVVNMYRRMCQRENIQFKKNLLVDN
                     ISIVLETPYDDARTK"
     gene            complement(8779..9003)
                     /locus_tag="VLB45_00640"
     CDS             complement(8779..9003)
                     /locus_tag="VLB45_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008301527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04246.1"
                     /translation="MSDNNPLKKSSRKELFAKGTIIAIIISVPSLVGFFVAWRLLDNL
                     IEAAVIGLVVHFVAMGFALKIAKKILGKKF"
     gene            9101..9562
                     /locus_tag="VLB45_00645"
     CDS             9101..9562
                     /locus_tag="VLB45_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010191048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HSD04247.1"
                     /translation="MASYKKRYSNDKSLDFIIPIMVLLFLGVVYIMSLRASEGYVSFI
                     LIGIAALVMIYWVRVLKKMTKSEQPQYTAKESETKNWVYDLIKGEQEIVFVAEVPGPE
                     DMVTVRLIDGILYIRGSGRFSKEVPIEVTPDMGIHDFKYRNGVLTLRIKKL"
     gene            complement(9557..10663)
                     /locus_tag="VLB45_00650"
     CDS             complement(9557..10663)
                     /locus_tag="VLB45_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007551254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HSD04248.1"
                     /translation="MKLKSKSKAAKTAEAKKLDLLSLLKNQGAYVYNDLNNGRFPQFS
                     IPSRSVSNIVYDKKLRQYILGKSAAVRSSRNMSQLRSFTQLAWLAFFVNRLVKEGKTS
                     TLRDIYYSSQAFAIDFEDQPESDNIIVDLEAIFAHAREDFHIFPEERSSVFGNLTIEY
                     TVPGYEGKRMNLSDHPDGYLIGPSLSSAELVETDAELVIAIEKGGLFTRFVEEKVHKK
                     FKALIVDTAGQAPRSTRYLLKRLSTELGLPVVILADGDVYGEHIAMVIKSGSANAAHL
                     RELTVPDAKWLGVWATDIEKYKLPTIPMTESDIKRIYELQKDPRYQEGNWKKQLEVFL
                     KLKRKAELEAFSKYGLTNITDKYLPEKLELAKSL"
     gene            complement(10641..>11703)
                     /locus_tag="VLB45_00655"
     CDS             complement(10641..>11703)
                     /locus_tag="VLB45_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010191050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA topoisomerase VI subunit B"
                     /protein_id="HSD04249.1"
                     /translation="ITKVRSELGLQRKNLNAKGILERAEKRWSNLSRPVRIVIAMMSF
                     LNLDFDQLNKIRIDDLDIANKRLTYWNFGESQSHAIELDPDSQYYKQLANTVAGDTLH
                     TFLTKRVQRVGPSTAEKFCEFAKLKSEKRIGSMTNEELVRLADSLQKFEEFLSPDPSC
                     LAPLGEEPLSKGIMKFFNPEFASVIQRSASAYSGFPFIVEMGIAYGGDIPPQGLKVYR
                     FANRIPLLYDEGSDVVLKVVDEVDWSRYKIKGDPPLVIVSHICSTRIPYKTVGKENVA
                     DRPELERELKNALQFLARKLAVHMSRQGMADMAKKRANLYSKYVPLIAQFATELSGNK
                     KEPNYKQLIQKGSEIEEQV"
BASE COUNT         3723 a         2178 c         2161 g         3641 t
ORIGIN      
        1 agaaagatgc aatctgcacc aaaatcacta gaaaaaacaa tagacaaact tcaaggatca
       61 ggattcaagg caagtccaac gagtcttaac ccatccggat ttaaaacaga tgcaaagata
      121 gaagaaatct tacaattact caattaagga ctgtagccta ggcagataaa gtaaagttca
      181 ctgcttgtct ttctacttgc ttttggtttt cttagttgaa cttttgtaaa tttttttcga
      241 atataatccc taaactcatt agaatattct ccatcaaaca ccttgaacaa agcattgccc
      301 ttgtatgcaa ggacatgttc aattattttt gctgcagaat aatttagcgc aacctgtttt
      361 gcatgatcaa ctgcccacac tccacttacc tgtggcgaca aatcacaaat taccgcattt
      421 acttttctgc caaagtgaga aaagactagc tccactatag attcatcctc aatgtttcct
      481 cgtacgatgt gcgcatttgg aatttcctca acatacgaag tatctattcc catgaccttg
      541 cctttgttac cagccaactc gacggcaact tgtgtccagc caccaggaga acatccaaga
      601 tctagcacaa aaaaacctgg acctattatt cgatatgatc tgttaagctc ttttagtttg
      661 tatgcagctc tactgcgata gccttcttgg tgtgctaatc ttcgatagta gtcttttcgc
      721 gcatcgatta atttcatttc tttgttttct tattggcaaa ttcagtgatt acccattgtt
      781 caattaacgg acatgttctt ggactaactt caccctcaag actgcatttt gattcaactg
      841 gacaaattaa acatggtgct gcctcaattg ttgcagtacc aactggaagt ctcgatattt
      901 ttagtttgta tgtccatcta ccattgtcta aaactttttc acgttttatg atgtttcgtt
      961 tctcaagttt tagagcaaat cttgaaccat ctctacttgt cagtttcaat tttttccaca
     1021 gttcactttg caacattcct tcaccgccac gttctgcaac aatgttacag acccggtttg
     1081 tgagtttttc catatcaaga ccctcaatct taaagttctc gatttcctca tcagatatca
     1141 cttcctccaa ttcctcttct accttctttt taggaaccag tttcttttca ggtactggtt
     1201 ttttctgagc agtagatttt ttttcaggtg caggtttttt ctgagcagta gatttttttt
     1261 cggatactaa tttttttaca agaattggct ttttttcagg agtttttttc ttctcgacca
     1321 atttttttac ttcatctttt ttgattggtt ttttgtcttt ctttttagcc atgatgatct
     1381 ctctataata gatactcagt tagatgtttg taagtaatat ataacatagt atttgtttta
     1441 ttgaactctg aacttggcag tggcacttac agttttttcg tagtctttat cattcatttc
     1501 taccacgatt tcatattctc ctgatttgcc aactttttca gaaaacgtgt cacctattgg
     1561 aagaagcacg tttccttcag aaaagcactg gttgaagaat ccattttgcg ctatgacgtc
     1621 ttttggagca acagtatcgt aaattgtgat atacagatct ccacaatcaa agtctagatt
     1681 gctgactaga actttaatct ctacaggagt agaagtagaa tattgtttca gaaggttttg
     1741 gatctgaatg tcttcgtttc caactggagg ttttgtagcg atttcaaaag gccacatgtt
     1801 gagctctcgg tttggttctg tcaaaactcc tgacaaaaca aatgtaccaa aagccgccga
     1861 tataacaact ggaaatagaa aaacaagcaa agctgtgttt tgcactgcca ttttgtaatc
     1921 taacactttt cattccctta tatctattta gtcaaaaagt ctcatgacac tctcatacaa
     1981 ttttggcgaa taagttaaat cgtggatagg caaatagccg ctagatgcga ctgagaaaat
     2041 tcagagtccg tgcatttaga tgtattcatg attctggcga tataagagtt ggagatttag
     2101 ctgcgtttat tggaagaaat gagagtggaa aaactacaat acttgaagct ttaacattgc
     2161 tcaacaagga tgcaaaagct tctgagcttg atttgtgcga tgaaatgtca gatgaattaa
     2221 aatcagaaat taaattagtt gaaggtgatt ttgatcttag tgaaacagag accaagctga
     2281 ttcaagacaa gtttccaaat ctatcagata taaaaagaat aaagatcttc agaacccaaa
     2341 ctaatccaaa gatacagtat gattttggag acgtaaaaat aagcggtgaa gcaaacaaaa
     2401 gaataaactc ttgggagaat tttgtagaaa agattcacaa ctttgtatca acaataccaa
     2461 accccatacg cataaagtta gatacaaaat ttcttgaggg tcacgcacca agaacaagag
     2521 atgcctttaa caatatgatg gcggaattta acaatcaaat ctatctcata gcatcagaag
     2581 aaagacaggt gatttcttcg tgggaggaga tataccgaga tctgggcaat acatatgata
     2641 gcctactaat aggaacaaac gagcgttctg cccttgagaa ttttgtagaa gaaaaactgc
     2701 acccaaggtt tgtttacttt tcagactaca agaagatttt aggcaatatt gatcttgatg
     2761 aatttttgaa agagacaaaa ggaataaggc caaaagggtt ggagtttatc gaagagtttg
     2821 acaagacaga gactgtcaaa aacttgtttt atttggcaga gcttgatcct gacaaactag
     2881 aggaagttca aaacacgcct tctaggctga taaaactttt gcacacttca agtagaaagt
     2941 taaccgagag actcaatcca gcttggaagg gagatccaat tcatgtagaa ttaagattca
     3001 accctggaaa catactaagt gttgtaattt ctgacgttca cagagacggc actgttacaa
     3061 acactggtct tttgaatcga agagcagaag gattcaagtg gacattttct ttcatagtaa
     3121 actttgcagc agagacacaa agatcagaac taaaagaagc gatactgctt cttgacgaac
     3181 ctgcgcgaaa cttgcatccg actcaacagc gtggaatttc agatttatta aaaggtcttg
     3241 caggctccaa tcaaattttg tatgcaacac actcaccctt tatgatattt gattacacgc
     3301 caggaaactt gcttgttgta gagttagata aaagaaaaca cctcagtcac atatattacg
     3361 actattggaa tgctgacgat gccacactag ttccaatatt gtatggtctt tcaagaggtc
     3421 ttgttgaatc aattcctgac agggaaatcg ggtccaattc aagaccagta attattgtag
     3481 aaacaatggt agactgtatg tatctaaatg cgtttgacaa attcttgaag gatcccaatc
     3541 tttccatgaa tccacttaac atagttccag catataacaa gaattctgtt cttccgcttg
     3601 ccatattcta cagaaatcat ggctacaaca cgttcgtgct tcttgacaac acagagctgt
     3661 cacgacagat ctctacaatg ctacaatcaa acgcattcaa gccagttcag ataatatttt
     3721 ttgaacttgg agggcaggca aaacaagcaa tcgaagattt gatagtagat gaagattacc
     3781 tatatgcagt taaccaaacc tatgaaatca aactcagaaa ggaaggatac cataacctta
     3841 caaaagaaga tgttgtgtca aaaggaagaa agagcatact tgataaccta agtgacatct
     3901 ggagagaaaa ccagcattta ggatgggaaa aatttgaacc agaagagatt tgtaggtaca
     3961 tttgtcaaaa aatcgcgtta ggtgaaacag actttttgtc ggacaagacc aaagaccaat
     4021 tcagagcaat ataccgatta attgctgaaa gaatcagaca aaaccaaaac attgtttctg
     4081 aaacaatcaa tacaaaataa ttcgagtttt tgaaatcagc tataattagt actttactca
     4141 taattgatac gattcaaacc gttaaaaaat gaaaaagaga cttaagattt aatttggagt
     4201 aaaattgtgt tttagtgtgg cagctacatt gtacaagggt attcttgtcg cgattttact
     4261 tgtttgtagt acattttctg ttcttacaac accggaggtt cgtggagcag atgaacttgt
     4321 aacagtagac gctacaaatc atggagatgt cacggttgta gaatacaaaa acagtcaaga
     4381 aaacattttt gatgtcaagt cagtagtttt ggaaattgac aaaggtggaa atttcaagtc
     4441 atttaagaca gaaaaaggat gggttggaaa aaggacatct gatgatacaa taacatttac
     4501 ttctaccatt ccaataaagc ctggtcaatc tgccaaattt ggaataaaaa ctgacaagac
     4561 agaacctgtc ttcaaatgga aggcttatga tgaagaaggc gatgaacttg gttctggtgg
     4621 agagattaca actagccagc caaaagaaaa cactagtgga ggcaacacgc aaaacggatc
     4681 ttcaggagtc ttagatggcg caacttttag attaattcct tctactccaa aagttgacac
     4741 atctttacgt atcataggag aaggctttgc tccaagcgag aaacttgact tttacataga
     4801 caataataga attgattctt ttgtgagtga tgaaaatgga aatttcattc taagtacaaa
     4861 gattccagaa aatcagaaag cagacagagc agatttcatc atcaaagatc aagcagggaa
     4921 tcaaaaaagc atgagtctaa gaatagaaga gtcttctaag agagttggca ttcaggacat
     4981 agaactaaca ataactggag acaaggtctt ttacagaggt gaggaaaaaa caatttcggg
     5041 aactgccgaa cctggcgcta cattaactat ttcaatatca gactctagtg gccaagtttt
     5101 aacatctttt acagaaaagg cagatagctt aggaaaatat gctataaaac acagagtccc
     5161 agcggaccgc gcgtttggcg aatacaccat aacaataaca gacggtaaaa gtacggtgtc
     5221 acacacttat gcagtagaga caacacaaaa aattattctt gcaacaacaa agcagagata
     5281 tgatcctggt gaaacgatcg taatcaacgg cactgctttg ccagatcagg aaatagaaat
     5341 catcatagaa gatccagttg gcatggaagc ttattcaaaa aatatggtag ttggttctaa
     5401 tggaatagta aatttagaat atccattaga agtttctgct agagaaggca cttacgtaat
     5461 atttgctact cagggagatc aacatgaaac gtttgttgtt ggagttggag aaattccaga
     5521 aattcagctt atagcaagaa tgagtgctat aaactacaag actacagaac aagcagttat
     5581 tttcataaca ggtccaccat catctacagt tagtctggtc ttagtggatc cttcagacaa
     5641 acaaaagttt gctgatacca taacagttgg agctgatggg tatgccgaat acagctttgg
     5701 tttaacgagt tatagtccag gagtatacac cgcagtactt tcaagaggaa atgcacaggt
     5761 tgaggaaaaa ttctcagtag gattacaaac tggttcaggg ccaataactc ttagaactgt
     5821 acgcgatact ttttctgcgg gggaaactat tctaatcata ggagaatcta atcctaacat
     5881 aatcataact gtcactttca aggatcctaa cggtacaaaa ataaaatctg cagacttgtt
     5941 tacagataaa aaggggacat tcacatcaaa cgcgttcaaa ttccccagtg atgccaagcc
     6001 aggaatttgg gtactggatg cagtaagcgg actaaatcat gttactaaag aaattacttt
     6061 gacaacagga acagatggtg aacttgcagt ttgggtagac aggagtccac caacatatga
     6121 taatgatcag attgttacaa taactggtag cggagcaggt caatctcaca atatcataat
     6181 agacatattt ggagcagatc agagcaaggt tgaatcactt agcatagttg caactggtac
     6241 tggtggcttt agcacattat ggaaagtacc tattagcttg aatgctggaa catacaccat
     6301 caaagttata gatgcaacaa agacagcaga gataaccata actgtacagt gacagcacca
     6361 aaattagaaa tactttttac atccacattt tgcctagctt gtgtgaatct taaagagaaa
     6421 atatcagagt ttcctgattt tccaaaacca ggaatcttgt ttagagatat cagtccctta
     6481 ttgaaagatc caaaagctat ttcctttatt gcagatgaat tttctaagat ctataaatca
     6541 aatgacgtgg atgttcttgc tggaattgaa tccagaggat ttcctcttgc atgtgcttta
     6601 gcattaaagt acaaaaaagg aatgatcatg atacgcaagc aaggaaaatt gccaggtaat
     6661 acagaaaaaa tgtcatacag tatagaatat ggcaatgcta caatggagat acaaagtgat
     6721 gcagtaaaaa atggacagaa ggtgttagtt tgtgatgatt tgttggcaac gggtggaact
     6781 gccaaaactg ccgcagaact aatagaaaaa ataggtggca aggtaactgg atttgcattc
     6841 atagtagagc tgacagacct gaaaggttca gacctcatta agaaatacag acacaaatcc
     6901 ttggtgaaat attgatggaa caggcagaga taggcatctt tggaggaact gggatctatg
     6961 attcaggatt gctcacagaa agcaaagaaa ttactataga cacgcctttt ggaaaaactt
     7021 cagattccat aacggttgga atgttcaaag gaaaaaaagt tgcctttatg ccaagacatg
     7081 gaaaaaaaca caccattccc cctcacttga ttaatttcag agcaaacatt tgggcattca
     7141 aagaaatggg cattaaaaga ataattgcac cttctgccgt aggaagccta aaagaaaaat
     7201 tgaagcctgg cgattttgcg ttacctacac agtttatcga tttcacaaaa tcaagaaaat
     7261 attctttttt tgaaggagat aaggtaattc acatatcagt tgcagatcca ttttgtccag
     7321 aacttcaaag tgcagtaagt aaagctgctg acaagatgaa tgtcaagatg cataaaaact
     7381 gcacatatgt ctgcatcgaa ggtcctaggt tttcaaccag agcagaatcc aaattttaca
     7441 aggaggtgat aggagcagac ataattggca tgaccctagt tccagaatgt caacttgcaa
     7501 gagaagccca aatgtgttat gtttcagttt caactgtaac agactatgat gtatgggctg
     7561 aaaaaccagt aactgcaaaa gaggttattg caacattgac aaaaaacgta gagactacaa
     7621 aaaaactact tacacttctt ccagatctta ttccacaaca tagaagatgt acctgtgaaa
     7681 aagcgttgac agaagcacaa ttttagttgt ctgcaaacat ttcttttttt attccctcag
     7741 gaatagtgat gtctccatgt actagtttat gctgcaatac atcaatcacc tcattagcgt
     7801 catatcctaa ttttttgagg gttttttctg tggtttttga tatttttgct ccaatgttat
     7861 gtccacctat ccttccaaat gcgattgcat tgaatctgaa acttttccca tcaagcgtaa
     7921 agacccccgt gatctttgca gctatttggc ttccatgaac attgttgaca tgaacactta
     7981 gttccaaatt ctcagatttc cactattttg ttaacagagt cttctatgag aaagttccag
     8041 tgtttttcat cttgtacttt aaagtcattt acttttagtg gaattatttt ttcatctgca
     8101 cattcgtgtt ggatttgatc atctttcttg agtttaacaa gtttccacat ctccttgtgt
     8161 ttttttaact tacatacaat aacggaccac ttttgatctg aatttatctt aggcattcct
     8221 acttcatcta ccaaagtttc tatgccaagt tgtttttctt cgcaaaagtg ttttttacac
     8281 agaacacatc gccatatatc cactgaacca attgttcttt tttcatagtt tatgtttgtg
     8341 actccaaagt aaactacttg atgattacat gaatcctgat ctacagccat tgatcttcac
     8401 cagatacagt tctatttagt tcttgcatca tcgtacggcg tttctaaaac aattgaaata
     8461 ttatctacaa gaagattttt tttaaattga atattttctc tttggcacat tcgtctatac
     8521 atattcacaa cagcaaaccg cgggtgcatg gattcaaact ctgtctttgt tatatcaata
     8581 aatcctccaa gatcgattaa tttttttgcc gtctccaatg catcctcctg cgatattcta
     8641 agactttggg caatgtcttc tgcagtcatt tttccttttt ttataacaag cacgaatact
     8701 ttggcctgaa gtggagagag ttttacttca gaaacaagcc tttcttcaac gatatcaaga
     8761 ttcacgctca taatacaatt agaatttttt acctagtatt ttttttgcaa tttttagcgc
     8821 aaaacccata gcaacaaaat gtaccacgag tcctattact gcggcctcga tcagattatc
     8881 cagtaaacgc cacgctacaa aaaaccctac aagtgatggt acgctgatta tgatagcaat
     8941 tatggttcct tttgcaaaca attcttttct tgagctcttt tttagaggat tgttatcgct
     9001 caaattgttc actagatgca tagatgacta taaaaatttg acatcgaaaa aaatccaaga
     9061 tcctttatat ttggtctgat ggtgaagaaa ttagttcgag ttggcaagct acaaaaagag
     9121 atactcaaat gacaaatcac ttgactttat cattccgatc atggtgttgt tgtttcttgg
     9181 agtggtgtac ataatgtcat tacgtgcttc tgagggatat gtcagcttta ttctgatcgg
     9241 tatcgctgca ctggtgatga tttattgggt tagggtacta aagaaaatga caaaaagcga
     9301 acagccacaa tacactgcaa aagaaagcga gacaaagaat tgggtatatg atctcataaa
     9361 aggagaacaa gaaattgtgt ttgtagctga agtaccagga cctgaagaca tggtaacggt
     9421 aagattgatt gatggaatat tatacatacg tggttctggt agattttcta aagaagtacc
     9481 cattgaggtc acaccagaca tgggaattca cgacttcaag tacagaaacg gtgtacttac
     9541 tctacgaata aaaaaactat agactctttg ccaattccag tttttctggt aagtacttgt
     9601 cagtaatatt tgtaagacca tattttgaga aggcttcaag ctctgccttt ctttttagct
     9661 tcaagaaaac ttcaagttgt tttttccaat ttccctcttg gtagcgtggg tctttttgca
     9721 gttcatagat tcttttgatg tctgattctg tcattggaat tgttgggagt ttgtattttt
     9781 caatatctgt tgcccataca ccaagccact ttgcatcagg aacagtaagc tcacgcagat
     9841 gtgctgcgtt ggcagaacct gattttataa ccatggcaat gtgctcgcca tacacatcac
     9901 cgtctgcaag tataacaact ggcaatccca gttcagtgct gagccttttt agcaaatatc
     9961 tggtagaacg aggagcttga cctgcagtat ctactatgag tgccttgaat tttttgtgta
    10021 ccttttcttc aacaaatctt gtaaaaagac cacctttttc aattgcaatg acaagctctg
    10081 catcagtctc aacaagctct gcactgctaa gacttggtcc aatcaaatat ccgtctggat
    10141 gatctgacag gttcattctt tttccttcat atcctggcac agtgtattcg attgtcaaat
    10201 taccaaaaac gctgcttcgt tcttcaggaa atatgtgaaa gtcttctctt gcatgagcaa
    10261 atattgcttc aagatcaact atgatgttat cagattctgg ctggtcttca aaatctatgg
    10321 caaatgcttg tgacgaataa taaatatctc taagtgttga tgtttttcct tcttttacaa
    10381 gtcggtttac aaaaaatgca agccatgcaa gctgtgtaaa agaacgcagc tgcgacatgt
    10441 ttcttgaact ccttactgct gcagatttac ccagtatgta ttgtctgagt tttttgtcat
    10501 atacaatatt gctgactgat ctgctaggaa ttgaaaactg cgggaatctt ccattgttaa
    10561 gatcattata aacataggca ccttggtttt tcaaaagaga aagcaaatct aatttttttg
    10621 cttctgctgt ttttgctgcc ttagacttgc tcttcaattt cacttccctt ctgtattagt
    10681 tgtttataat ttggttcttt tttatttcca gaaagttctg ttgcaaactg agctataagt
    10741 ggaacatatt tggaatagag atttgcgcgt ttttttgcca tgtctgccat gccctgtctt
    10801 gacatgtgta ctgccaattt tcttgcaaga aactgtaatg catttttgag ctcacgttct
    10861 agttctggtc tgtctgcaac attttccttt ccaactgttt tgtatggaat tcttgttgaa
    10921 catatgtgag atacaatgac aagaggcgga tcacccttaa tcttgtacct actccaatct
    10981 acttcgtcta caactttgag tacaacgtca cttccttcat cgtataagag tggaatcctg
    11041 tttgcaaagc gatagacttt gagtccttgt ggtggtatgt ctccaccata agcaattccc
    11101 atctcaacaa tgaatgggaa tcccgagtat gcagaagcgc tacgctgtat aacagaagca
    11161 aattccggat tgaaaaattt cattatgcct tttgataatg gttcttctcc aagtggtgcc
    11221 aaacagcttg gatctggtga caaaaattct tcaaattttt gaagcgagtc tgccagtctg
    11281 acaagttctt cgtttgtcat actgccaatt cttttttctg attttaattt tgcaaattcg
    11341 cagaattttt ctgctgttga tggtcccact ctttggactc tttttgttag aaaggtatgc
    11401 agcgtatccc ccgctactgt atttgcaagc tgtttgtagt attgactatc tggatctagt
    11461 tctattgcat gggattgtga ttcaccaaaa ttccaatatg taagtctttt atttgcaatg
    11521 tcaagatcat ctatacgaat tttatttaat tgatcaaaat ctaggttaag aaaagacatc
    11581 attgctatga ctattctaac tggtctggaa agattagacc accgtttttc tgctctttcc
    11641 aatatgcctt ttgcgtttag attttttctt tgtaatccta gttcacttct tacttttgta
    11701 atc
//
LOCUS       DATIDL010000013         3537 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrosopumilaceae archaeon isolate SMAG_U16342
            k141_2242468, whole genome shotgun sequence.
ACCESSION   DATIDL010000013 DATIDL010000000
VERSION     DATIDL010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935836
            Sequence Read Archive: SRR25158326
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosopumilaceae archaeon (soil metagenome)
  ORGANISM  Nitrosopumilaceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrosopumilales;
            Nitrosopumilaceae.
REFERENCE   1  (bases 1 to 3537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/04/2024 05:09:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,520
            CDSs (total)                 :: 1,474
            Genes (coding)               :: 1,474
            CDSs (with protein)          :: 1,474
            Genes (RNA)                  :: 46
            rRNAs                        :: 1 (5S)
            complete rRNAs               :: 1 (5S)
            tRNAs                        :: 43
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3537
                     /organism="Nitrosopumilaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2242468"
                     /isolate="SMAG_U16342"
                     /isolation_source="soil"
                     /db_xref="taxon:2202732"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(208..1167)
                     /locus_tag="VLB45_00660"
     CDS             complement(208..1167)
                     /locus_tag="VLB45_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HSD04250.1"
                     /translation="MNITEYQTHCPECQSALIDDVHNGEKICSGCGLVAMEQMSDYGP
                     ESRGTNLEDKMKLARATGQTTYSQHDLGIRTEIAMGTKDFSGKSINAEMVNQMYNLRK
                     WQTRIRVASPRERRLANILSKIGETCQVLGLSRNVLESASMIYRNFEGQFEAKGKSVA
                     CMSAATIYMACKQCDVVRSLDEICKATGSAKEDKMNVKLTAKYYRTLVMELGSTTAPV
                     VTLDKYISKIANLAKLEVRVERLAAEIAEKTQDHTLADGKAPNGLAAAYLYIASILLG
                     QSVLQRDVSSIAGITEVTIRNRCKEILTAFKMRVTLRPSLVKN"
     gene            complement(1164..1412)
                     /locus_tag="VLB45_00665"
     CDS             complement(1164..1412)
                     /locus_tag="VLB45_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007549955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gar1/Naf1 family protein"
                     /protein_id="HSD04251.1"
                     /translation="MHLASSGRVIIRLTKQVRDGQLLVDDAGRKIAKVSEMIGPVDAP
                     YASATPLTNNIQKYIGKKIFMGEETPAIRPRRFKGRKR"
     gene            complement(1509..1808)
                     /locus_tag="VLB45_00670"
     CDS             complement(1509..1808)
                     /locus_tag="VLB45_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048500 - signal recognition particle
                     [Evidence IEA];
                     GO_function: GO:0008312 - 7S RNA binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle subunit SRP19/SEC65
                     family protein"
                     /protein_id="HSD04252.1"
                     /translation="MKDYEHIVVWLDYFNKTLPKKMGRKLAKEKSVFDPSLKELSDAA
                     KAAGFVPTEENDQVRFPKRPFVRSGYIVLPKGVPKSKILQKISEKLVAKRAKQSK"
     gene            complement(1813..2193)
                     /locus_tag="VLB45_00675"
     CDS             complement(1813..2193)
                     /locus_tag="VLB45_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007401227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8e"
                     /protein_id="HSD04253.1"
                     /translation="MKKSVENLATSKITGGRRYPLRTRRKYETDRYPVETLLGPQEII
                     TRKVRGKNTKGALKTTDFCNLAMPGAKVKKTKILKVLKNSTNNDYERRGVISKGAILE
                     TEDGQCRVVSRPGQSGIVNAILIK"
     gene            complement(2289..2858)
                     /locus_tag="VLB45_00680"
     CDS             complement(2289..2858)
                     /locus_tag="VLB45_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HSD04254.1"
                     /translation="MVKVENDDEASARLEAALYSAGRPLTMEELIRASGTESRTKTLA
                     LMTNLIKRAKSAFKAIEVASLPDGSYVFQLKPEFNTVIRKYASKPLLPTATLKTLSYI
                     AYMQPISSKQLVEIRGSGVYLHLRLLHQLDYIEHQNVGRMKIYTTTEKFQKYFGIQGD
                     KDSLKQRLFTKVRNVPQTPPPTQEPALQA"
     gene            complement(2927..>3537)
                     /locus_tag="VLB45_00685"
     CDS             complement(2927..>3537)
                     /locus_tag="VLB45_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="chromosome segregation protein ScpA"
                     /protein_id="HSD04255.1"
                     /translation="IDISKKDVWAIDIIRILELLIKILNQTGKKDLRVAGIAALSSAM
                     IHRMKVESIFALQKAAMEKKPLRQRLDIDVETIDMPYRHEATYPVTLDELLTVLENLI
                     GTIANPRSSRRQMQFEPVEVPDFNDYFIGLEKIIGQYEDLIVNKIRATGSGSFRMIVS
                     ELETIDIVRCFFAILFLARDEKVELEQLENDIIVSLVPSLVK"
BASE COUNT          858 a          764 c          678 g         1237 t
ORIGIN      
        1 agattgtcat caagataaaa ctgagcgaga aagaaattct aaacgttaag cacttttact
       61 gttaaattat tttcataaat ttccaagttc tcgtacccaa tgtcagttaa aaaaccatcg
      121 gactcttaga tttatgcata aattttatca gatgattgaa tacaaataca caactatctt
      181 aatttaataa aacaaaaaga agaggggtta gtttttgact aacgacggtc gcagtgtaac
      241 acgcatctta aaggcagtaa gtatttcctt acacctgttt cgtatcgtaa cctcggtgat
      301 tccagctatg cttgagacat ccctttgcaa aacgctttgt cctagtaaga ttgacgctat
      361 gtaaagatac gcagcagcca atccgtttgg cgccttgccg tcagcaagag tatggtcttg
      421 cgttttttca gcaatttctg cagctagtct ctcaactcgc acttcaagct ttgccaggtt
      481 tgctatcttt gagatgtact tgtccagggt tactacgggc gcggttgtgg agccaagctc
      541 cattaccagg gttctataat attttgcagt gagttttacg ttcatcttgt cttcttttgc
      601 gcttcctgta gctttgcaga tctcgtctag tgatcttacc acgtcgcact gtttgcacgc
      661 catgtaaatt gtggcagcag acatgcatgc gactgatttt ccctttgctt caaactggcc
      721 ttcaaagttt ctatatatca ttgaagctga ttccagtacg tttcttgaca gccctaatac
      781 ttgacaggtc tccccaatct ttgataatat gtttgctagt cgtctttctc ttggcgaagc
      841 aaccctgatt ctggtttgcc acttgcgcag attgtacatc tggtttacca tttcagcgtt
      901 gattgacttg ccgctaaagt cctttgtgcc catcgcaatt tctgttctga ttcctaagtc
      961 gtgttgggaa taggtagtct gtcctgttgc ccttgcaagc ttcatcttgt cctcgaggtt
     1021 tgtgcccctt gattctgggc cataatctga catttgttcc attgcaacca aaccgcagcc
     1081 agaacaaatt ttttctccat tatgaacgtc atcgattaat gccgactgac attctggaca
     1141 gtgtgtttgg tattctgtta tgttcatctt tttcttccct tgaatcgtct tggtcttatt
     1201 gcgggggttt cttcacccat gaaaattttt tttccaatgt atttttggat gttgtttgtc
     1261 agcggagtcg cagatgcata aggtgcatca acaggaccta tcatttctga tactttggca
     1321 atttttctac cggcatcatc aaccagtagt tggccatccc ggacttgctt agtgagtctt
     1381 ataatgactc taccgctact ggccaaatgc aaaacttcgc ctaccccctg caattagttt
     1441 ctcaaacaaa atactgttat caatagagtt ctgtcaaaat tactttagca acaagctaga
     1501 agactagttt attttgactg ctttgccctt tttgcaacaa gtttctcgga aatcttttgc
     1561 aggatttttg attttggaac accctttggt agaacaatgt atccggatct tacaaagggt
     1621 cgctttggaa atcttacctg gtcattttct tctgtcggca caaatccagc tgcctttgca
     1681 gcatcggata attctttaag cgacggatca aaaacacttt tttcttttgc aagttttctt
     1741 cccatttttt ttggcagggt cttgttgaaa taatcaagcc agactacgat gtgttcgtaa
     1801 tctttcatgg aatcacttta ttaaaattgc atttacaatg ccgctttgtc cgggtcttga
     1861 cactactctg cactgtccgt cttctgtttc tagtatcgcg ccttttgaaa tgacaccgcg
     1921 tctttcataa tcattgtttg tagaattttt cagaactttt agaatttttg tcttctttac
     1981 ttttgctccc ggcattgcca agttgcaaaa gtctgttgtc tttaatgctc cctttgtatt
     2041 tttaccgcgc accttgcgtg ttatgatttc ttgcggtcct agcaaagttt caacaggata
     2101 tctgtcagtt tcatattttc ttctggttcg aagtggatat cttcgtccac ctgttatctt
     2161 acttgttgca agattctcta cagatttctt catgacttgt ccattaaaga actctaattt
     2221 ataccatgtt tcagttttga atttaaaata tctattttta aatttacaaa attacaagac
     2281 acgcaaaatt atgcctgtag tgctggctct tgggttggtg gtggcgtttg tggtacgttg
     2341 cgaactttgg tgaaaagcct ttgtttgagc gaatccttgt ctccttgaat tccaaagtat
     2401 ttttggaact tttctgtagt ggtgtagatc ttcatccttc caacattttg gtgctcgata
     2461 taatcaagct ggtgcaaaag cctaaggtgt agataaactc cagaaccgcg tatctcgact
     2521 agctgctttg aggatatggg ctgcatgtat gcaatataag ataaagtctt gagggttgcc
     2581 gtaggcaaga gaggctttga ggcatatttt ctaatcacag tgttaaactc gggttttagc
     2641 tgaaagacat acgagccgtc tggcaggctt gctacctcaa ttgccttgaa tgctgacttg
     2701 gctcttttta tcaggtttgt catcaatgca agggtcttgg tccttgattc tgtccctgat
     2761 gccctgatta gctcttccat ggtgagaggt cttcctgcag agtatagtgc tgcttcaagc
     2821 ctagcgcttg cttcgtcatc gttttctacc tttaccattc gtaaaaaatt gtgccaaaat
     2881 gtacttaaaa gaatccttta cgggcatttg atcatattgt gaacttctat tttactaaac
     2941 ttggtaccaa actaacaata atgtcgtttt caagctgttc tagttctacc ttctcgtctc
     3001 ttgcaagaaa caggatggca aaaaaacacc ttactatgtc aattgtttca agttcggaaa
     3061 ctatcatcct aaaagaacct gaacccgtgg cacgtatctt gtttacaata agatcctcgt
     3121 actggccaat tattttttca agccctatga aataatcatt aaagtctggc acctcaactg
     3181 gctcaaactg catctgcctg cgacttgagc gcgggtttgc tattgttcca ataagattct
     3241 caagaactgt cagaagctca tctagcgtga caggatatgt agcctcgtgt ctgtacggca
     3301 tgtctattgt ttctacatca atgtccagtc tttgcctgag cggttttttc tccatcgctg
     3361 ccttttgcaa agcaaatatg ctttctactt tcatcctgtg tatcattgca gaagacagag
     3421 cagcaattcc tgcgacccga agatctttct tgcctgtctg gtttagaatt ttgattagca
     3481 gctccaagat ccgtattatg tcaattgccc agacgtcttt ttttgatatg tctatgg
//
LOCUS       DATIDL010000014         5615 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrosopumilaceae archaeon isolate SMAG_U16342
            k141_227427, whole genome shotgun sequence.
ACCESSION   DATIDL010000014 DATIDL010000000
VERSION     DATIDL010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935836
            Sequence Read Archive: SRR25158326
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosopumilaceae archaeon (soil metagenome)
  ORGANISM  Nitrosopumilaceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrosopumilales;
            Nitrosopumilaceae.
REFERENCE   1  (bases 1 to 5615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/04/2024 05:09:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,520
            CDSs (total)                 :: 1,474
            Genes (coding)               :: 1,474
            CDSs (with protein)          :: 1,474
            Genes (RNA)                  :: 46
            rRNAs                        :: 1 (5S)
            complete rRNAs               :: 1 (5S)
            tRNAs                        :: 43
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5615
                     /organism="Nitrosopumilaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_227427"
                     /isolate="SMAG_U16342"
                     /isolation_source="soil"
                     /db_xref="taxon:2202732"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            739..1374
                     /locus_tag="VLB45_00690"
     CDS             739..1374
                     /locus_tag="VLB45_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008297293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Snf7 family protein"
                     /protein_id="HSD04256.1"
                     /translation="MTSFAKSWTQPQTPSLSEKINDAIKPQGPLKPRLETAVKKLQTQ
                     ISKLDSLLTKLKQKDEKLFKRIVTATQQHDTTTSRVLSKELAEVRKVTKLLGNARIAL
                     EQIELRLTTFHDLGDTVVTIMPTIGLMKSLRSSLLRFMPEADHELSGMTEMLSGLMTD
                     SFHGGDFGIDSGATSEESEKILQEAAAVAEAAVNDKLPSMPASQSSSARYI"
     gene            complement(1371..2639)
                     /locus_tag="VLB45_00695"
     CDS             complement(1371..2639)
                     /locus_tag="VLB45_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007550725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSD04257.1"
                     /translation="MSFAQGRKWIWYLLPSQITAEGLRTVIPLYIIFLGGGINEVAVI
                     VALHYGASAFGSLFWGRIIDRFHVRRTVLLISLSATLLSCVWLYFTKDLGLLLVISPI
                     IGFFIVGKNPVTHLLVMESAPKNQWSWLFARISIIATLGMLGAMVIGTVWSVYFDLGP
                     YFLICALSSGIAIVFTNTIKESVFHLERGSIAHSIQGLRYSFSHFHYVFPKIPELYDY
                     KHIITIFKGKVSHEIGILFLSTFLFYLGSNIYFTAYTPFLKHFGFSDSTVFLVYTIQT
                     ITMITIFFVAPKIIAKFGEERSTMIAYVPRVAAVLVAGFVVPFMFGGGVFVLSIISVC
                     LMVLAFSIFSTSSSMILFKSIPQGFEGKYLGVNSSVTGIGVFIGALVTGKITSSFGYT
                     ISFLVSAIVLVMAFVLFRVYLHYKLSHNTV"
     gene            complement(2792..2920)
                     /locus_tag="VLB45_00700"
     CDS             complement(2792..2920)
                     /locus_tag="VLB45_00700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04258.1"
                     /translation="MSDAVKEKPFRRRIETVPWDEKLPRDGDKGHHVFQSPKGFNL"
     gene            3144..3455
                     /locus_tag="VLB45_00705"
     CDS             3144..3455
                     /locus_tag="VLB45_00705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04259.1"
                     /translation="MIYESNLETKLEQILIGLSKDKTSPKVKEAISKFLLYVQHASEN
                     FWITFHRAKTYQQRLDCYYQFTKNQCLATEVLIRDLNSIMDEDEIKENLNAILKEGFT
                     F"
     gene            complement(3452..4009)
                     /locus_tag="VLB45_00710"
     CDS             complement(3452..4009)
                     /locus_tag="VLB45_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014966109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HSD04260.1"
                     /translation="MFEKFRKNLEKFRELSSEGEGNVMVKDDYGEQKFESAESKHVEQ
                     IKIPEHVEQVSMTQSGDDIGIKSLMDKRAKLEEAIDYVGLMIKNLKDKRTKLEKNIED
                     ESVDIKNLKEKLIKVGQYIDEEKQGIRNLSQKRADVEKEADDVGVIINNLREKLTTID
                     HVVDEEGNKIKNFKESRSNLESSLS"
     gene            4209..4547
                     /locus_tag="VLB45_00715"
     CDS             4209..4547
                     /locus_tag="VLB45_00715"
                     /inference="COORDINATES: protein motif:HMM:NF014047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HSD04261.1"
                     /translation="MGYNEGGFGDSSDNRGYSGDRGYGGGRRFGGGRRFDDRPKPVET
                     GKEYDVSITEISRKGDGIARVDGFVIFVKGGQVGQNAKVKITQVGGRFATADVVGEGS
                     PQPAAEPTAE"
     gene            complement(4598..4981)
                     /locus_tag="VLB45_00720"
     CDS             complement(4598..4981)
                     /locus_tag="VLB45_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6659 family protein"
                     /protein_id="HSD04262.1"
                     /translation="MKNENHKLDLVPIVEDILAIDPSMRFAAIIDLKGNISEAIMKEG
                     KTSLKSQKEEEHFCKQVALRRKIRNKFNKSLGKVDYVHIERERVTQIVLYPKRKTVYV
                     TMEPKLDITRKLEIVDLIKKKTVQL"
     gene            complement(5018..5584)
                     /locus_tag="VLB45_00725"
     CDS             complement(5018..5584)
                     /locus_tag="VLB45_00725"
                     /inference="COORDINATES: protein motif:HMM:NF012312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSD04263.1"
                     /translation="MCVFVMTGLVYMYHGSVGFLPTLLQQFGHIDRIHVAPIMISATI
                     SSWLGMILTGWLSQKIGRKKSMILFAAGSIIFVIPLAYGILGGDLMATILFVIIYAFI
                     VSTASGPVPAFFSERFPTEVRNSAAGFAYNGGLIVGSWSPLIAVSLISLVKVEFIPIA
                     LGVNIMIGAIILLVGTLLNPETKDRDLT"
BASE COUNT         1894 a         1056 c          935 g         1730 t
ORIGIN      
        1 agggcccttt ccgcaacgtg ggcaacgacc gccaccacta aatgattcgc gcgtcatatg
       61 atatctaata aaagtttcga tcgaacggca ataaaaagat ttaattatta tggcgtattc
      121 gattattttc atatattata atataatgat ataaaatcta gaaatttcaa aacaaaaatt
      181 ataatttcta taatttgtga cctttctcta agaatgaatc atacaaaaat caattacttc
      241 aaaaaacatg tttcttgtaa tttcttgatt cttgagtatt ttatctcaaa tgagacaaac
      301 tgaattttgc aaatcaagaa ataatctttg acaaactttt acggattgta aaaactgatt
      361 tttacaaaaa atgtttccaa actgtccaaa gcaaaaactt aaatacaatt tttttgattc
      421 tcaggcatta aataaaatgg aaaaatgtcc actttgctgt gaaccacagc aatcatcatc
      481 tgcattgctg gttcacataa agaaaatcca tccgcagaga aacagcatac aattagtgca
      541 ctgatctctg gtctgaggat agatggggag gatgtatcca atttaggtta tattgaactt
      601 tctagaaaaa tcaatcttgt ctgaatttta cttgttacgc gtctaaccaa taggctgttg
      661 tttttgttag ctaaatttag tctgcagtga acccgatttc acaccttgaa ttaataacga
      721 atttttttac tcgttatcat gacaagcttc gctaaaagct ggacacagcc acagacacca
      781 agtcttagtg agaagattaa tgatgcaata aaaccacaag gtccactaaa gccaagattg
      841 gaaacagcag taaaaaaact gcagactcag atttctaaat tagatagctt acttacaaaa
      901 ttaaaacaaa aagacgaaaa acttttcaaa agaattgtaa ctgcaacaca acaacatgat
      961 accacaacta gtagagtttt atcaaaagaa cttgctgaag tgcgtaaagt aacaaaactc
     1021 ttaggaaatg ctagaatagc acttgaacag atcgaattaa gattaacaac attccacgat
     1081 cttggcgaca cagtagttac catcatgcca accattggtt tgatgaagag cctaagatca
     1141 tcactcttaa gattcatgcc agaagcagat catgagctaa gcggtatgac tgaaatgcta
     1201 agtggactca tgaccgattc attccacggc ggagactttg gtatcgattc tggagcaaca
     1261 agtgaggaat cagaaaagat tctacaagaa gcagcagcag tagcagaggc agcagtaaat
     1321 gacaagctac catcaatgcc tgctagtcaa tcctcttcag cacgttacat ctagactgta
     1381 ttgtgcgata gtttgtagtg aagatatact cggaacaaga caaaagccat tactaaaaca
     1441 atagcagaaa ctagaaacga aatcgtatat ccaaaagaac ttgtaatttt tccagtcact
     1501 agagccccaa taaatactcc aattccagtt acagaactat ttactcccag atattttccc
     1561 tcaaaacctt gtggaatact cttgaataaa atcatggagc ttgaagtact aaaaatagaa
     1621 aatgctagta ccattaagca tacagaaata attgataaaa caaaaacccc tcctccaaac
     1681 ataaatggaa caacaaaacc tgcaacaagt actgctgcta ctcttggtac atatgcaatc
     1741 attgtagaac gttcttctcc aaattttgct attatctttg gagccacaaa gaaaattgta
     1801 atcatggtta tggtttgtat tgtatacacc aaaaatacag tagaatctga aaaaccaaaa
     1861 tgtttcaaaa atggagtata tgcagtaaag taaatgttac ttcctaaata gaaaagaaag
     1921 gtagacaaaa ataagattcc tatttcatga gaaacttttc ccttgaatat ggtaattatg
     1981 tgtttgtagt catacagttc aggaatcttg ggaaaaacat agtgaaagtg actgaaacta
     2041 tagcgcaaac cctggatgga atgtgcaata ctacctcttt ccaagtgaaa cacggattct
     2101 tttattgtgt tggtaaatac aatagcaatt ccacttgata atgcacaaat caaaaaataa
     2161 ggcccaagat caaaatacac actccagaca gtgccaatca ccattgctcc taacatgcct
     2221 aaggtcgcaa ttatcgaaat tcttgcaaaa agccaactcc actgattttt aggtgcagat
     2281 tccatgacaa gcagatgcgt tactgggttt tttcctacta tgaaaaatcc aattattggt
     2341 gaaatcacta aaagtaaccc gagatctttt gtgaaatata gccatacaca tgataacaac
     2401 gtagctgaaa gcgatatgag aagaactgtt ctccttacat ggaatcgatc tattattctg
     2461 ccccaaaata atgaaccaaa tgcagatgct ccatagtgca atgcaacaat tacagcaact
     2521 tcgttaatcc cgcccccaag aaaaattatg tataatggaa taacagtacg taatccttca
     2581 gcagtaatct gcgacggtag gagataccag atccattttc tgccttgggc aaacgacatt
     2641 gtttctatac tctcaaaaat taacgcacta ttgtacgatg catttgaata ctttccacag
     2701 ttttgtcgat tttaatttca tagcaaagat tgcatttttg gtattgggaa aaattgtgtc
     2761 atgcagaagc gtgttggata acacgcctga gctaaaggtt gaatcctttc ggactctgaa
     2821 acacatgatg acctttgtca ccatctcttg ggagtttttc atcccatgga actgtttcaa
     2881 tccttctgcg gaagggtttt tccttgaccg catcgctcaa ctgcacgact cctatcttgg
     2941 gaggatgttc ctttgatagg gatatactat acgtgacaaa attatttaag ttttgcttag
     3001 ttattctttt tgaaacgtac aattttttgt ttaaaatcaa ataactacac aaaaacatga
     3061 tctttcctgc aaattctatg atgaacattt ttatcatatg aatagtatag catttcataa
     3121 caaaagaaat cgacatctac cagatgatat atgaatcaaa cttggaaact aaactagaac
     3181 agatactgat aggtctttca aaagacaaga catctccaaa agtaaaagaa gcgatatcaa
     3241 aattcttgtt atatgttcaa catgcgtcag aaaatttctg gataactttt catcgtgcta
     3301 agacatatca acaaagatta gactgctatt accaatttac aaaaaatcaa tgtcttgcaa
     3361 ccgaagtcct aattcgagat ctaaactcta taatggatga agacgaaata aaagaaaatc
     3421 ttaatgcgat tttaaaagaa ggctttactt tttagctgag agaactttct aagtttgaac
     3481 gtgattcttt aaagttcttt attttattac cttcttcgtc tactacatga tctattgttg
     3541 tgagtttttc acgtagattg ttaatgatga cacctacatc atcagcttct ttttctacat
     3601 ctgctctctt ctgggacaag ttcctgattc cttgcttttc ttcatctatg tactgaccaa
     3661 ctttgatcag cttttctttc aaattcttga tatctacaga ctcgtcctca atattcttct
     3721 caagttttgt acgtttgtcc tttaggttct taatcattaa accaacataa tctatggctt
     3781 cctcaagttt tgcacgcttg tccatgagtg attttatgcc tatatcgtcg cctgactggg
     3841 tcatactgac ttgttcaaca tgttctggta tttttatttg ttcaacatgc ttggattctg
     3901 ctgattcaaa cttttgttct ccatagtcat cttttaccat gacatttccc tctccctccg
     3961 aagaaagttc tctaaacttt tctaggtttt ttctgaactt ttcgaacata tatctaaaaa
     4021 taatgtaatt tacaatatat aaaaagataa tagatcagac ttgtcaattt catgacccta
     4081 attctagttg attttatttt ataacaaaat ggtatttcta actaaattgc ccagatagta
     4141 aaattaagta gctgatcaga ttttataggg catagagcct gcatcaaggt aaggaaggag
     4201 gtgtaacgat gggctataac gaaggcggtt ttggtgacag ctctgataac cgtggataca
     4261 gcggcgatag aggctacggc ggcggtagac gatttggcgg cggtagacga tttgatgaca
     4321 gaccaaaacc agtagagacc ggaaaagaat atgacgtgtc tataacagaa attagtagaa
     4381 aaggagacgg tatcgcaaga gtagacggct ttgtgatatt tgtaaaaggc ggacaagttg
     4441 gacagaacgc aaaagtaaag atcactcaag taggtggtcg atttgccact gctgatgtag
     4501 taggagaagg ttctccacaa cctgcagcag aaccaacagc tgaataagca gtctgattaa
     4561 tccaaaaact aaatttcttt tcctaatttt tcttatttta taattgaacg gttttctttt
     4621 ttattaaatc aacaatttct aattttcttg taatgtctaa ctttggctcc attgtaacgt
     4681 acacagtctt tcttttagga tacagcacaa tttgtgttac cctctcacgt tcaatgtgaa
     4741 cataatctac cttgcctaaa ctcttgttaa acttgtttcg tatttttctt cttaacgcaa
     4801 cttgtttaca aaaatgctct tcttcttttt gtgactttag tgaagtttta ccttccttca
     4861 ttattgcttc tgatatgttc cctttcagat ctatgattgc ggcaaatctc atgctaggat
     4921 ctattgccaa gatgtcttcc acaataggaa ccagatctag cttatgattt tcatttttca
     4981 agatatgaat gccaaatctt gattcgataa atatacctta ggtaagatca cgatcttttg
     5041 tttctgggtt aagtaacgta cctacaagca aaattattgc tcctatcatt atattcacgc
     5101 ctaaagctat gggaataaac tcaactttaa ctaaagaaat caggcttact gcgatcagag
     5161 gagaccatga accaactatt aatcctccat tgtatgcgaa tcctgctgca ctgtttctaa
     5221 cttcagtagg aaatctttca gaaaaaaatg caggcactgg tccagatgct gtagaaacta
     5281 tgaatgcata aatgattaca aagagaattg tagccattag atctcctcct agtattccat
     5341 aagctagtgg aatcacaaag atgatagaac cagccgcaaa gaggatcatg gatttttttc
     5401 gtcctatttt ttgactaagc cagccagtta ggatcatgcc aagccaagat gaaattgtcg
     5461 cagaaatcat tattggtgca acatgtattc tgtctatatg tccaaattgt tgtaatagtg
     5521 ttggcaaaaa accaactgag ccatgataca tgtaaacaag gccagtcatt acaaaaacac
     5581 acaaaaagaa tgcctttctg tgttatttgc caaat
//
LOCUS       DATIDL010000015         3067 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrosopumilaceae archaeon isolate SMAG_U16342
            k141_631661, whole genome shotgun sequence.
ACCESSION   DATIDL010000015 DATIDL010000000
VERSION     DATIDL010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935836
            Sequence Read Archive: SRR25158326
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosopumilaceae archaeon (soil metagenome)
  ORGANISM  Nitrosopumilaceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrosopumilales;
            Nitrosopumilaceae.
REFERENCE   1  (bases 1 to 3067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/04/2024 05:09:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,520
            CDSs (total)                 :: 1,474
            Genes (coding)               :: 1,474
            CDSs (with protein)          :: 1,474
            Genes (RNA)                  :: 46
            rRNAs                        :: 1 (5S)
            complete rRNAs               :: 1 (5S)
            tRNAs                        :: 43
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3067
                     /organism="Nitrosopumilaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_631661"
                     /isolate="SMAG_U16342"
                     /isolation_source="soil"
                     /db_xref="taxon:2202732"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..591)
                     /locus_tag="VLB45_00730"
     CDS             complement(<1..591)
                     /locus_tag="VLB45_00730"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HSD04264.1"
                     /translation="MGTQMSAARRGIATEEMKVVARDEDVTLEWLLPKIASGSIIIPH
                     NNERPQKIRVVGIGKGMKTKVNVNIGNSTLSNNLEEEIKKAKVAVKYHADTIMDLSDG
                     GDVGLVRRTLLEAAPITFGTVPVYEAYNYGVEKFKNPLNITEDDFLNAFERNIKDGVD
                     YTTIHSGITKEIAKRILAVKRHGGIVSKGGTITAAWM"
     gene            645..1949
                     /gene="thiD"
                     /locus_tag="VLB45_00735"
     CDS             645..1949
                     /gene="thiD"
                     /locus_tag="VLB45_00735"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007401276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HSD04265.1"
                     /translation="MHILSIAGSDPSSGAGIQGDLKTFSALGAYGLTVITTITSQNTV
                     RFQSVEKISPAMIKNQIESVLSDFKIDFIKIGMVYSSPIIKAIYSKLRKLKIPIVLDP
                     VFESTTGGVLLLNDALDDFKKLLLPLAFVITPNIPEAEKLAGMKIRTESDVRRAAYKI
                     RDMGARNVIIKGGHLKGKLVTDFVLEKSKFYSFSGKRIHRTSHGGGCGFSASLAVSLA
                     KGNSLRESVRFAKEFTEKSIKDSEKIGSGISIAKTYRQDKIEKNLADAISDFIYIKDI
                     YKIIPECQTNFVFSKPNPKSLDDILGVSGRIVKADKAVVMAGNLKYGGSKHVASAVLE
                     VARKFSNIRSALNIKYDAPLIRKAIAKKLSVHSYDRSLEPVRTKAREGSSMTWGIRSA
                     LKNANRPPDLIFHKGDFGKEPMILIFGKEPRDVLGKLAKIVE"
     gene            1995..2696
                     /locus_tag="VLB45_00740"
     CDS             1995..2696
                     /locus_tag="VLB45_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007550006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HSD04266.1"
                     /translation="MKAVILAGGLGTRLQPYTTFLPKAMLPLGEKPLLEHLIEWIRKN
                     NIDSIVLCVSYLRKTIEDYFEDGNRFGVKIEYAVANRPLATAGQLKTAEEFIDDSFVC
                     VYGDSIFDFNLKNMIDYHKRKKSFVTMALFEYKTKLQYGFIETDKNNKVTAWKEKPEV
                     KGNINVGCYVMEPNVFKIIPPNKPFGMDSVIKSAIARKQKVSGFLIKKGFIDIGDKIS
                     YRRAYQHYLEKLGQI"
     gene            2706..>3067
                     /locus_tag="VLB45_00745"
     CDS             2706..>3067
                     /locus_tag="VLB45_00745"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HSD04267.1"
                     /translation="MTDVKIRELEEKDLSSGFLESLDSLRKASDLDPKKAKEIFKKIK
                     SDPNYTIYVAVLDSKVIGATTLLIEQKFIHKGGRVGHIEDVVVRKEYQGKGIGQKLVK
                     ALSEHAEKKGCYKTILDCT"
BASE COUNT         1032 a          562 c          615 g          858 t
ORIGIN      
        1 catccatgcc gcagttattg ttccgccctt acttacaatg ccaccgtgtc tttttactgc
       61 aagaattctt tttgcaatct cttttgtaat accagaatgt atcgtagtgt aatcaacgcc
      121 atcttttatg ttcctctcaa aggcattgag aaaatcatct tcggttatgt taagtggatt
      181 cttgaatttt tcaactccgt aattgtacgc ctcataaact ggaacggtgc caaacgttat
      241 cggtgctgcc tccaagagcg ttcgtctcac cagcccaacg tctcccccat cgcttaggtc
      301 cattattgtg tctgcgtggt acttgactgc aactttggct ttttttatct cctcttcaag
      361 gttgtttgat aaagtagaat tgccaatgtt tacgttcacc tttgttttca ttcccttgcc
      421 aatgcctaca actcttattt tctgtggtcg ttcgttgtta tgcggaatga ttattgagcc
      481 gcttgcaatc tttggcaaaa gccattccaa agtgacatct tcgtctcttg caaccacttt
      541 catttcttca gttgcaattc cacgacgtgc tgcactcatt tgcgttccca tgtttcattt
      601 tacataacga ttggatttaa acgtgagttt tggatcatta ttttgtgcac atattatcaa
      661 tagcaggatc tgatccttct tcaggggcag gcattcaagg ggatttgaaa acattttctg
      721 cgttaggagc gtatggtctt acagttatta ctacaattac aagccagaac actgtaagat
      781 ttcaaagtgt ggaaaaaatt tctccagcca tgataaaaaa ccaaattgaa tcagtcttgt
      841 ctgattttaa aatagatttt ataaaaattg gaatggtgta cagctcgcca atcataaaag
      901 caatttattc taaactaaga aagctaaaga taccaatagt tttagatcca gtttttgaat
      961 caacaacagg tggtgttttg ctactaaatg acgcgttaga tgattttaaa aaattgcttt
     1021 tgcctctggc ttttgtcata actccaaaca taccagaagc tgagaaactt gcaggcatga
     1081 aaataagaac agaaagcgat gtgagacgtg cagcgtacaa gatacgagac atgggtgcaa
     1141 gaaatgtcat catcaagggc ggtcatctca aaggcaaact agtgacagac tttgttctgg
     1201 aaaaatcaaa attttactca ttttctggaa agagaataca tcggacaagc cacggtggag
     1261 gatgcggctt ttctgcatca cttgctgtca gtcttgccaa aggcaacagc cttagggaat
     1321 cagtaagatt tgccaaagaa ttcacggaaa aatcaataaa agattcagaa aaaattggta
     1381 gtggaatttc aatagcaaaa acgtacaggc aagacaagat agaaaaaaat cttgcagatg
     1441 caatttctga ttttatttac ataaaagaca tttacaagat aataccagag tgtcaaacaa
     1501 actttgtttt ctcaaaaccc aatccaaaat cactagacga catacttgga gtgtcaggca
     1561 gaattgtcaa ggctgacaag gcagttgtca tggccgggaa tctgaaatac ggaggttcaa
     1621 aacatgttgc atctgcagtg ttggaggtgg caagaaaatt ttctaacata agatctgcgt
     1681 tgaacataaa atatgatgcg ccactaatta gaaaagcaat tgcaaagaaa ctttcagtac
     1741 acagttacga tagatctctg gaacctgtaa gaacaaaagc aagagaaggt agcagcatga
     1801 catggggcat acgaagtgca ctcaagaatg caaacaggcc accagatctt atatttcaca
     1861 aaggagactt tggcaaagaa cctatgattt tgatatttgg taaagaaccg cgtgatgttc
     1921 ttggcaagct tgctaaaatt gtagaataga atttcatcct tcaacataaa tacccataat
     1981 cgtaaataga attcgtgaag gcagtaatcc ttgcgggcgg actgggcaca aggcttcaac
     2041 cctatacaac ttttcttcca aaggcaatgt tgccacttgg ggaaaaacca cttcttgaac
     2101 acctcataga atggattaga aaaaacaaca tcgattcaat tgtactgtgt gtaagctatc
     2161 tgagaaaaac gatagaagat tattttgagg atggaaacag gtttggagtc aagatagaat
     2221 atgcagtagc aaacaggcca cttgcaacag caggacagtt aaagacggct gaagagttta
     2281 ttgatgattc ctttgtgtgc gtttatggtg actctatttt tgattttaac ttaaaaaaca
     2341 tgatagacta tcacaagaga aaaaaatctt ttgtgacaat ggcgctgttt gaatacaaaa
     2401 ccaaactcca gtatggattt attgaaactg ataaaaacaa caaagttact gcatggaaag
     2461 agaaaccaga agtaaaagga aacatcaacg taggatgtta cgtcatggag cctaacgtat
     2521 tcaaaataat tccgccaaac aagccatttg gaatggacag tgtcataaaa agtgcaattg
     2581 cacgaaaaca aaaagtgagt ggttttctaa tcaaaaaagg cttcatcgat attggggata
     2641 aaatctctta cagaagggca taccagcact atcttgaaaa gcttggccag atttgatgct
     2701 aaattatgac tgatgtaaag atacgtgaac ttgaagaaaa ggatctttcc agcggctttc
     2761 tagaatcttt ggattcatta agaaaagcaa gtgatttaga cccaaaaaaa gcaaaggaga
     2821 tattcaagaa gataaagtcg gatccaaact atacaatcta tgtagcagtg cttgattcaa
     2881 aggtgattgg agctacaact ctgctcatag aacaaaaatt cattcacaaa ggagggcgag
     2941 ttggacacat tgaggatgtt gttgtaagaa aggaatacca aggcaaaggc attgggcaaa
     3001 aattagtaaa ggcgctctca gagcatgcag aaaagaaagg atgctacaaa accatacttg
     3061 attgcac
//
LOCUS       DATIDL010000016        10329 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrosopumilaceae archaeon isolate SMAG_U16342
            k141_2044045, whole genome shotgun sequence.
ACCESSION   DATIDL010000016 DATIDL010000000
VERSION     DATIDL010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935836
            Sequence Read Archive: SRR25158326
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosopumilaceae archaeon (soil metagenome)
  ORGANISM  Nitrosopumilaceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrosopumilales;
            Nitrosopumilaceae.
REFERENCE   1  (bases 1 to 10329)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10329)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/04/2024 05:09:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,520
            CDSs (total)                 :: 1,474
            Genes (coding)               :: 1,474
            CDSs (with protein)          :: 1,474
            Genes (RNA)                  :: 46
            rRNAs                        :: 1 (5S)
            complete rRNAs               :: 1 (5S)
            tRNAs                        :: 43
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10329
                     /organism="Nitrosopumilaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2044045"
                     /isolate="SMAG_U16342"
                     /isolation_source="soil"
                     /db_xref="taxon:2202732"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..917
                     /locus_tag="VLB45_00750"
     CDS             <1..917
                     /locus_tag="VLB45_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HSD04268.1"
                     /translation="LRLACGLDSIVVGNEEVLEEIKTSISNAKLTKTSGRILNKLFDS
                     SIRIATRIRDSTGISKGVMSIGDVAVKIAEEYAGLDGKKRVLLIGTGEAAAMVAKPLN
                     KKKYAFDISSMTIERSTGFSKVLGGKPMTFEDVLSNFDKFDIIFVATTADYFIITYDK
                     IKVIMENKTKGTMILDISDPRAVDEKVSTLPGTKLMFRDQIIEMEERNLKARKDEVLA
                     VEKMISKEVPILEATMKRLEPEPIVKDVFSSVDSLRKKELEKALQMLGETDEKKIKII
                     DELTKAVAESIVSVPVSNSKKALEQDKQ"
     gene            1001..1954
                     /locus_tag="VLB45_00755"
     CDS             1001..1954
                     /locus_tag="VLB45_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HSD04269.1"
                     /translation="MSLLLKDRVYSMESTTTKRGVYPLHGYKLGLYRLPIKLEEPLEI
                     KSIVEGLKKTFAMDTFADRIYVTYTWTEENMSDPDAKGYEKVDLSITVEIVTGEVVDL
                     IYQIFPIEKFGDPQWVKDYRKKADHYAKMLIDTILRNTILSDKMIGYYVKTQKLGQDA
                     AIEKLEELTPLAKIVPDAKPKLKAEEPQDATTAPKDVGGPLTAPKPGPIDVEYKSHMG
                     SSPSFVVPSNKNVIKTWGKGSSNGRLGVWGEYVSVDFDICVADGACIDACPVNVYEWF
                     DTPGHVSSEKKPLMAREPDCIFCLACEGVCPPQAIKIFQRK"
     gene            complement(1962..2420)
                     /gene="bcp"
                     /locus_tag="VLB45_00760"
     CDS             complement(1962..2420)
                     /gene="bcp"
                     /locus_tag="VLB45_00760"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HSD04270.1"
                     /translation="MLTVGDTVPDFNLKNAEGQTVKKSDLKGKKYVVYFYPKDFTPGC
                     TTEAAEFARDYKKFKNIGIEIIGISPDDVESHKKFGEKMEVPFILLADTETETAKKFG
                     VWGKKQFMGREYMGVNRTTFLVDEKGKIFKVFEKVKAAGHAQEVLEAFKN"
     gene            2517..3221
                     /locus_tag="VLB45_00765"
     CDS             2517..3221
                     /locus_tag="VLB45_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007401294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP family channel protein"
                     /protein_id="HSD04271.1"
                     /translation="MVNPRAWFAESVATFALVFFGPLSVTLAAVAFGTDLSIEGIIMI
                     SLGHGAAIGLMIYTFGHVSGAHINPAVTISMIVTKRIGIKDGVAYIIFQLIGAIVAAA
                     AHKAILPELGAKVNFGTQGGPSALLNHDVMAGLGVEMILTFFLVTVIFMAAVHKKAAS
                     GFAGFAIGGMIFLIHLVGVPLTGASVNPARTFGPALISGFWEFHWIYWVGPIIGGIIA
                     GLIMNYVFVKKAEQQA"
     gene            3280..3354
                     /locus_tag="VLB45_00770"
     tRNA            3280..3354
                     /locus_tag="VLB45_00770"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3315..3317,aa:Arg,seq:gcg)
     gene            3473..4327
                     /locus_tag="VLB45_00775"
     CDS             3473..4327
                     /locus_tag="VLB45_00775"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HSD04272.1"
                     /translation="MGKVKTSNNGKKSEKTTMFQKMHEHFMQVAPKYRELRTTDLGPI
                     MFIANDLREMESIVAADVGCGAGRYTLKFLQHLGEKCHMYCIDSNLNMLRHLKEHLTR
                     NNIVNFLPIKSDSHKIPLPTDSLDCIMTLNAIHHFSLQPFLKEASRVLKNNGKLFIYT
                     RLRDQNARTIWGMHFPDFHKKEKRLYELDHLKSMFERDPNLNLDSIKIFEHHRVFPLE
                     RLIEQARNHHYSTFKFYRKKEFNEALHRFKENILHHFDDPRKIGWKDENILLVIDNFQ
                     GGSFKQRK"
     gene            4376..4873
                     /locus_tag="VLB45_00780"
     CDS             4376..4873
                     /locus_tag="VLB45_00780"
                     /inference="COORDINATES: protein motif:HMM:NF013117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HSD04273.1"
                     /translation="MNIDAKILYAILSIIVTGAFFTTSHLIFKIKLHGLQGSYKLLHI
                     ISISIIISELVYLGDILGLFELALEVIASIGVAFALIALALQVHLKNVIAGIGLYLNP
                     EINIGDVIEIGGKQGVIIEFHLTKTTVLTEGGERMFIPNLKFNEEIIIISPKHAKPQQ
                     GVESN"
     gene            complement(4965..5444)
                     /locus_tag="VLB45_00785"
     CDS             complement(4965..5444)
                     /locus_tag="VLB45_00785"
                     /inference="COORDINATES: protein motif:HMM:NF013231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HSD04274.1"
                     /translation="MSYKNKIDLDNAIGLLIKSTSKSIEKSLDFELHRKCGLSSGQWK
                     VIGALAIQNGLTQSKIAEMVFVETPTLVPILDKMEKDGWVERKADPSDRRNNNIFLTK
                     KAEGSLVESVIDSVLGIRKMITKDITHAELEVTKKTLRQIMGNAESFLEKNNSKNMG"
     gene            5508..6260
                     /locus_tag="VLB45_00790"
     CDS             5508..6260
                     /locus_tag="VLB45_00790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04275.1"
                     /translation="MNTRIKLGIQYAGFAIALSALVWATQDLEFSAWAEESTDDKKSY
                     EPTTVVGDEIKNNPMAQKILKNIEIAKQRMEQMQQKQKQLTEQQKLVEEQRKIAKDRL
                     EKDLARMNKDYEDFTPKNSYFKFLSNNVNSTYHGIFLDQFNYMNERITIASLAKKAVL
                     DNGGTYAEAREEFNKYASMSRVDMIKYVQEINIKHGFTDEQLQSYFDKNGKLPRYEDD
                     EVTSCYGCEKYEKIKEEILEERLSQTNSTKTA"
     gene            6501..6674
                     /locus_tag="VLB45_00795"
     CDS             6501..6674
                     /locus_tag="VLB45_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1059 domain-containing protein"
                     /protein_id="HSD04276.1"
                     /translation="MLQLCCKDYGFDCEFIVQEENASKVIETFMNHTSEVHGIDYSKE
                     ALMQFVIRKTNYE"
     gene            6667..6927
                     /locus_tag="VLB45_00800"
     CDS             6667..6927
                     /locus_tag="VLB45_00800"
                     /inference="COORDINATES: protein motif:HMM:NF024248.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HSD04277.1"
                     /translation="MNETEEDQIPIILDPHSKMILDITCNLPKSVSEIKNECNIPLST
                     LYRKIRKLHNYHLLKTMIKITPEGNKYALYKSNKTKPLNFLH"
     gene            7022..7297
                     /locus_tag="VLB45_00805"
     CDS             7022..7297
                     /locus_tag="VLB45_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008300843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04278.1"
                     /translation="MKLVERGEINQTALFSFCGLNLTKHKPILDELELNGMVSRTETS
                     VGKRIITIYKPTQKGIEFCQDILEPYEKMFPRRKSSEVNKLSFLILV"
     gene            complement(7283..7669)
                     /locus_tag="VLB45_00810"
     CDS             complement(7283..7669)
                     /locus_tag="VLB45_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008300773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04279.1"
                     /translation="MNDTKDTSTSRQNILGLLDNFIEQIHNIRKILIGVSISAVILAP
                     LAIGLSIYLLLHPSFFAILEIENEFGLVLSILLAAVITISSIWLVTGIRQYRSINFLN
                     KRYDEYNREKDEIDRKIASQYGLDKD"
     gene            complement(7743..8231)
                     /locus_tag="VLB45_00815"
     CDS             complement(7743..8231)
                     /locus_tag="VLB45_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016940002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HSD04280.1"
                     /translation="MVKKQKIKTKNKISKNTVIGIGIVVVIAAIGGYFWINSLIPVNG
                     TAPLFTPPTNTFIVAKHSPQDGYVYVSKATTSGKKSVSGGSINPTIYLTKGSAESIHF
                     INEDNFAHSKHNINIDEFNVHSRDLGYFQTEAITFIADKEGSFHYYCSIHPEMKGMVI
                     VE"
     gene            8560..8856
                     /locus_tag="VLB45_00820"
     CDS             8560..8856
                     /locus_tag="VLB45_00820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04281.1"
                     /translation="MIFTNETILKVWKKAEIDEYNVPNDFRKDPCGAWIERTQYGNHD
                     SAYGWEIDHIVQQSSDVNNLSNLRPMHWKNNTHAEDGNPVCHIVSSGTINVDRI"
     gene            complement(8894..>10329)
                     /locus_tag="VLB45_00825"
     CDS             complement(8894..>10329)
                     /locus_tag="VLB45_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007403162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HSD04282.1"
                     /translation="ARLREIFKEAREKSPTIVFIDEIDSIAPKREEVTGEVERRVVSQ
                     LLSLMDGLEGRGKVIVIAATNRPNALDPALRRPGRFDREIEIRVPDKKGRHDILLIHT
                     RNMPLTEDVNLEKIAAISHGYVGADLEYLCKEAAMKCLRRLLPEINLADEKLPPETLD
                     KLIVNAEDYQLAFREVTPAGMREVFIEKPDIKWEDVGGLENVKRELQEAVEWPMKYPV
                     LYSKLGHTMPRGILLYGPSGTGKTLLAKAVATESEANFISVKGPELLSKWVGESERGV
                     REIFRRARQSSPCIIFFDEIDSIVPVRGMGESMVTEKVVSQLLTELDGIESLHGVVVI
                     AATNRADMIDRALIRPGRFDKIVLVPTPDRDGRVKILQISSRDIPIDRELKTKSGSKN
                     PDYVDLEKIADATDGLSGADVAAIANTAVSLVIHEFIYKYPDQKEAEKQAENTKVVMR
                     HFEEAVRKVRAQKDLKVGEKIAVSYYR"
BASE COUNT         3378 a         1858 c         1859 g         3234 t
ORIGIN      
        1 tattaagact tgcatgtggt ctggattcaa tagttgtagg aaacgaagag gtccttgagg
       61 aaataaaaac atcaatttca aatgcaaaac taactaaaac atctggcaga atattaaaca
      121 aattatttga tagttctata cgaattgcaa cccgcatacg agactctact ggaatcagta
      181 aaggagtgat gtctattggt gatgtcgctg tcaaaattgc agaagaatac gccggtttgg
      241 atgggaaaaa acgcgttttg cttataggta cgggagaagc agccgccatg gttgctaaac
      301 ctcttaataa aaaaaaatat gcatttgata tttccagtat gacaattgaa cgatccaccg
      361 gtttttcaaa agtattgggc ggaaaaccaa tgacgtttga agatgttcta tcaaactttg
      421 ataaatttga cataatcttt gttgcaacca ctgcagatta tttcataatt acttatgaca
      481 aaataaaagt gatcatggaa aataaaacaa aagggacaat gattttggat atctctgacc
      541 caagagctgt tgatgaaaaa gtctctacac ttcctggaac taaattgatg tttagggatc
      601 aaattattga gatggaggaa agaaacttga aagctagaaa agatgaagtg cttgctgtag
      661 aaaaaatgat tagtaaagaa gtcccaatcc tagaggcaac aatgaaacga cttgaaccag
      721 aaccaatagt taaggatgta ttttccagcg tagattcgtt aaggaaaaaa gaattagaaa
      781 aagcattaca aatgctagga gaaactgatg agaaaaaaat caaaattatt gatgagttga
      841 caaaagctgt tgcagaaagt attgtttctg tacctgtgag caattcaaaa aaagcattag
      901 aacaagataa acaataacta agcaaatttt agattgtttt tgtattttct agcgattcta
      961 aatagcgaag ctttttaaat aaattaaaca aaattacaac atgtcacttc tcttaaaaga
     1021 ccgagtgtac tcgatggaat ccacaaccac aaaaagaggt gtttatccgt tacatggcta
     1081 caaacttggc ctttaccgtc tgccaatcaa actagaggaa ccattagaga taaaatcgat
     1141 tgtagaagga ctgaaaaaga ctttcgcgat ggataccttt gcagacagaa tctatgtgac
     1201 atatacgtgg acagaggaaa atatgtctga tccggatgca aaaggatacg aaaaggttga
     1261 cctctccatt acagttgaaa ttgtaacagg tgaagttgtt gacctgatat accaaatctt
     1321 tccaattgaa aagtttggcg atccacaatg ggtaaaagac tatcgcaaaa aggcagacca
     1381 ctatgcaaaa atgttaattg atacaattct acgaaacacc attttgtcag acaagatgat
     1441 tggatactat gtcaagacac aaaaactagg acaagatgca gctatagaaa aactagaaga
     1501 actcacacca cttgcaaaaa ttgtaccaga tgcaaaaccg aaactaaaag ctgaagaacc
     1561 acaagatgct accactgcac caaaggatgt aggtggacca ctaactgctc caaaaccagg
     1621 tccaattgat gtggaataca aatcacatat gggttcaagc ccctcatttg ttgttccatc
     1681 aaacaagaat gtcataaaga catggggaaa aggatctagt aatggaagac tgggtgtttg
     1741 gggcgaatat gtgtctgttg attttgatat ctgtgtagca gatggcgcat gtattgatgc
     1801 atgccctgta aatgtctatg aatggtttga tacccctggc catgtgtcat ctgagaaaaa
     1861 gccattgatg gcgcgagaac cagattgtat attttgcttg gcatgtgagg gtgtatgtcc
     1921 gccacaagca ataaagatct tccagagaaa ataaaaaaat cttaattttt aaaagcctct
     1981 aggacttctt gtgcatgtcc tgcagctttt actttttcaa acaccttgaa aatttttcct
     2041 ttttcatcca ccaaaaaagt agtcctattt actcccatat actcacgtcc cataaactgc
     2101 ttttttcccc atacgccaaa cttttttgca gtctcagtct ctgtgtctgc aagtaagata
     2161 aagggaacct ccattttttc accaaacttc ttatgggatt ctacatcatc tgggctaatt
     2221 cctattatct caattccaat attcttgaac ttcttataat ctcgtgcaaa ctcggcagcc
     2281 tcggttgtac agcctggggt aaagtccttt gggtaaaaat ataccacata ttttttgcct
     2341 ttaagatcag attttttcac tgtttgacct tcagcgtttt ttagattaaa gtcaggtact
     2401 gtatctccaa cagtgagcat gggttatgca tctgccattc cctaattatt ttttcctagt
     2461 actgatcaaa aattatccga atgttttata tggcatatat cgaggtttga tgaataatgg
     2521 ttaatccaag agcatggttt gctgaatcag ttgctacttt tgcgttggtt ttctttggac
     2581 cactttctgt aacactggca gctgttgctt ttggtaccga tctctcaatt gagggaataa
     2641 taatgatttc acttggccac ggtgctgcaa ttggtcttat gatctatact tttggtcatg
     2701 tatctggtgc tcacatcaat ccagctgtga ctatttctat gattgtcact aaacgaattg
     2761 gaataaaaga tggagttgct tacatcatat ttcagttaat tggtgcaata gttgctgcag
     2821 cagcacacaa agcaattctg ccagaacttg gtgccaaagt aaactttggt acacaaggtg
     2881 gaccaagtgc attactaaat catgatgtaa tggcaggtct tggtgttgaa atgattttga
     2941 cattcttctt ggtaactgta attttcatgg cggcagtaca caaaaaagct gcctcaggat
     3001 ttgcaggttt tgcaattggt ggaatgattt tcttgataca cttggtaggt gtaccactca
     3061 ctggagcaag cgtgaatcct gcaagaacat ttggtcctgc attaatctca gggttctggg
     3121 agttccattg gatttattgg gttggaccaa taattggtgg aataattgct ggattaataa
     3181 tgaattatgt ctttgtaaaa aaggcagagc aacaagcata gattttgttt tctaaacatt
     3241 tttgaaatgt attataacgg gtagactcga ctttctattg gacccgttgc acagcttgga
     3301 tagtgcggac gcttgcggag cgtcaggtcg tgggttcgag tcccatcggg cccgttttat
     3361 ctatcagtac gagtccctct gggacaactt tttgattcat acatctgtgc aagaatttga
     3421 aacaatcgag aaccatgaaa actaataaaa atcatcacat cctaccttta gaatgggaaa
     3481 agtaaaaaca agtaacaatg gaaaaaaatc agaaaaaact acaatgtttc aaaagatgca
     3541 tgaacatttc atgcaagtag ctccaaaata ccgagagtta agaaccacag atttaggacc
     3601 aatcatgttt attgctaatg atcttcgtga aatggaaagt atagtagcag cagatgttgg
     3661 atgtggagca ggcagatata ctctgaaatt tttgcagcat cttggagaaa aatgtcacat
     3721 gtattgtatt gacagcaatc ttaacatgtt gcgtcactta aaggaacacc tcacaagaaa
     3781 caatattgtt aactttcttc ccataaaaag tgactcgcac aaaataccac tgccgacaga
     3841 ttctcttgat tgcataatga ccttgaacgc aatccatcat ttttctttac agccgtttct
     3901 aaaagaagcc tcaagggtgc tcaagaataa cggaaagcta ttcatctata ccagattgag
     3961 agaccagaat gcccgcacaa tatggggtat gcattttcca gattttcaca aaaaagaaaa
     4021 gcgtctttat gagttggatc atcttaaatc gatgtttgaa agagatccaa acttgaactt
     4081 ggactcgata aaaatatttg aacatcatag agtgtttcca ttggaaagac taattgagca
     4141 ggcaagaaat catcattact ctacgttcaa attctacaga aaaaaagaat ttaatgaagc
     4201 acttcatcgg ttcaaagaaa acatattgca tcatttcgac gatccaagaa aaataggctg
     4261 gaaagatgaa aacatacttt tagtaataga taattttcaa ggaggatctt tcaagcaaag
     4321 aaaatagtca cacacaatgt ttgaaattac tattgtctct tttgtgttgt cagatatgaa
     4381 tatagacgca aaaatccttt atgcaatttt atcaattatt gtaacgggag cgttttttac
     4441 aactagtcat cttattttta aaatcaaact acatggtttg caaggttcgt acaaactact
     4501 tcatattatt tcaatttcaa taataatatc tgaacttgtt tatcttggag atattttagg
     4561 cctatttgaa ttagcgttag aagttatagc gtcgatagga gttgcgtttg ccctgattgc
     4621 actggcactt caggtccatt taaaaaatgt aatagcaggc attggtctat atctaaatcc
     4681 tgaaattaac ataggcgatg ttatagaaat aggagggaaa cagggagtaa taatagaatt
     4741 tcatcttacc aagactactg tcttgactga gggaggagaa cgtatgttca ttcctaatct
     4801 aaaatttaat gaagaaatta taataatttc tcctaaacac gcaaaacccc agcaaggagt
     4861 agagtctaac tgatttgtga actaagttaa gagcactttc atctatcatg tgttaaggga
     4921 gtaaaattct ccacatcgtt ctcttacctg gcactgggct gtgcttaacc catgttttta
     4981 ctgttatttt tttctaaaaa tgattcagca tttcccatga tttgtcttag tgtttttttt
     5041 gtcacctcca gttctgcatg tgtaatgtct tttgtaatca tttttcttat gcctaaaaca
     5101 gaatcaatca cagactctac caacgaacct tctgcttttt ttgtaaggaa aatattgtta
     5161 ttcctacgat cactaggatc agcttttctt tcaacccatc catctttttc cattttatct
     5221 aatatgggca caagagtggg agtctctaca aaaaccatct cagcaatctt actttgggtc
     5281 aaaccatttt gtattgcaag tgcccctatt accttccact gaccacttga tagtccacat
     5341 tttctgtgta attcaaaatc gagagatttt tcaattgatt ttgacgtgga ttttattaaa
     5401 agacctatgg cgttatcaag atcaatttta tttttataac tcatttgatt agtctactaa
     5461 tcattagtaa gctaatactt atatcccttt atgtttaatg ctgtctaatg aatactagaa
     5521 taaaactagg gattcaatac gcaggatttg caattgcatt gtctgcatta gtatgggcaa
     5581 cacaggattt ggaattttca gcatgggcag aagaatcaac tgatgacaaa aaatcttacg
     5641 aacccactac tgttgtgggc gatgaaatca aaaataatcc aatggcccaa aaaattctca
     5701 aaaacattga gatcgcaaag caaagaatgg aacaaatgca acaaaaacaa aaacagctaa
     5761 cagaacaaca gaaactcgtt gaagagcaaa gaaaaattgc aaaggatcgg ctggaaaaag
     5821 atcttgcaag aatgaataaa gattatgaag attttactcc aaaaaattca tactttaagt
     5881 ttctttcaaa taatgtcaac tcaacttatc atggcatatt cttggatcaa tttaactaca
     5941 tgaacgagag aatcacaatt gcaagccttg caaaaaaagc agtcttggat aatggcggaa
     6001 catatgcaga ggcaagggaa gaatttaaca aatacgcatc tatgtcaaga gtggatatga
     6061 taaagtatgt tcaggaaata aacataaagc atggattcac agacgaacaa ctacaatcat
     6121 attttgataa gaatggaaaa ttgccacgat atgaagatga cgaagttact tcatgttatg
     6181 gttgcgaaaa atatgaaaaa ataaaggaag aaattcttga agaacgactc tcacaaacca
     6241 attctactaa aaccgcataa tcgttacatc tgactgcata aatgataaag aaattgatac
     6301 agctacaaac atggaattta gccttgatga tctgataaat agagcctagc tcttgttaga
     6361 gccaagcccg tccgtgaacg ccccaatttt agaggtgtgt ctaggttcgc agataaattt
     6421 cctatagtcc ataatcagta ataagtgtaa attaacaatg ttaagtaact gattttaaac
     6481 atcaaaaaac attttcttta ttgttacagt tatgttgtaa agactatgga tttgattgcg
     6541 aattcattgt tcaggaagaa aacgcgtcaa aagtgattga aacgttcatg aatcatacct
     6601 ctgaagtgca tggcatagat tattccaagg aagctttgat gcaatttgta attcgaaaga
     6661 ctaactatga atgaaactga ggaagatcaa atccctataa ttctagaccc acactcaaaa
     6721 atgatccttg acattacttg taatttgcca aaatctgttt cagagataaa aaatgaatgt
     6781 aatattccgc taagcactct ctatagaaaa attcgtaaat tacacaacta tcatcttctt
     6841 aaaacaatga tcaaaattac tccagaagga aacaaatacg ctctttacaa gagtaacaaa
     6901 actaagcctc tgaatttctt acattagaga taatgttata gaacatggtt tttttgagaa
     6961 taccatgtct ggttctggca gttcctacag ggacaggatt tacattatta aagatataat
     7021 cttgaagcta gtagagagag gagaaattaa tcagactgcc ttgttcagct tttgtggtct
     7081 gaatctaaca aaacacaaac caattcttga tgaattggaa ttgaatggaa tggtaagcag
     7141 aactgaaaca tcagttggaa agagaataat tacaatatac aaaccaactc aaaaaggtat
     7201 tgagttttgt caagacattt tagaacctta tgagaagatg tttcctcgca gaaaaagtag
     7261 cgaggtaaat aaattgagtt ttttaatcct tgtctaatcc gtactgtgat gcaatttttc
     7321 tatctatctc gtctttttct ctattgtatt catcatatct tttgttcaag aaatttatcg
     7381 agcggtattg ccttattcct gttaccagcc aaatagatga aatggttatg actgcagcaa
     7441 gaagtatgct gagaacaagc ccgaattcat tttctatctc tagtattgca aaaaaggaag
     7501 gatgcaataa aagatagatt gaaagtccta ttgcaagtgg tgccaaaata actgcagaaa
     7561 tagagacacc tatcaagatc tttctgatat tgtgtatttg ttcgataaaa ttgtctagca
     7621 gtcctaaaat gttctgtctt gacgtactgg tatctttagt atcattcaaa taaaaaacaa
     7681 cacctgaaag tttgaaaatg acttgataat attaaatgat ttgttcgatg actcaaatag
     7741 gatcattcca caattaccat tcctttcatt tctggatgga tactgcaata ataatgaaag
     7801 ctgccctctt tgtctgcaat aaatgtaata gcctcagttt gaaaataacc cagatctcgt
     7861 gaatgtacat tgaactcatc aatgtttatg ttatgttttg aatgtgcaaa attgtcttca
     7921 ttgataaaat gaattgattc tgctgatcct tttgttagat aaatcgttgg attgatactg
     7981 cctccactca cacttttctt tccacttgtt gtagctttac ttacatacac atagccgtct
     8041 tgtggtgaat gtttagcaac gatgaaagta tttgttggtg gtgtaaatag aggtgctgtt
     8101 ccatttaccg gaatcaacga gtttatccaa aaatagccac ctatagctgc aatgactact
     8161 actattccaa ttcctatgac ggtattttta ctaattttat tttttgtttt gattttttgt
     8221 ttttttacca caaaacaaat tttaggttat gaagtattta gacaatgatt aactttgtta
     8281 atctacagtc tatctccttg gcatttagaa agaatttcta tcatcatacc gggagcttat
     8341 tatagtaaca aaaggatata gagtgtgtca aaatctccag tagagtgcaa atgtgcttct
     8401 tgtgaatgtg gtatctacac gtataatgaa tccaaagttt gtgccagatg tgaagacaat
     8461 aagcattcat cgggtcagaa aagatggtcc actatgatat taaaaactga aatgaaaact
     8521 tcaccagtag aagaagccat gtagaatgag gcagtaaaaa tgatcttcac taatgaaact
     8581 attttaaaag tctggaaaaa agccgagatt gatgaatata acgttccaaa tgatttcaga
     8641 aaggatccat gtggagcttg gattgaaaga acacaatatg gaaatcatga ttctgcatat
     8701 gggtgggaaa ttgatcacat agtacaacaa tcaagtgatg tgaataattt atcaaatctt
     8761 cgtccaatgc attggaaaaa taatacgcac gctgaagatg ggaatcctgt ttgtcatatc
     8821 gtatcatcag gtacaataaa cgttgataga atctaaaatg atttggaact gatacaccta
     8881 aaatgtttga aacttatcta tagtaagaga cagctatctt ttcgcctacc ttaagatctt
     8941 tctgtgctct gacttttctc acagcttctt caaaatgtct catgactacc tttgtattct
     9001 ctgcttgttt ttccgcctct ttttgatctg gatatttgta gatgaactca tgtatgacaa
     9061 gagatactgc tgtgtttgca atagctgcaa catctgcacc gcttagacca tctgttgcat
     9121 ctgcaatctt ttctaaatct acataatctg gatttttaga tccactttta gtttttaatt
     9181 ctcggtcaat aggaatgtct cttgagctta tctgcagtat cttgactctt ccatccctat
     9241 ctggagttgg tacaagcact atcttgtcaa atctgcccgg tcttatcagt gctctatcaa
     9301 tcatatctgc tctgtttgtt gcagcaataa ccacaactcc atgtaaactc tcaatgccgt
     9361 caagttctgt caagagttgg ctaacaactt tttcggtaac catgctttca cccattcctc
     9421 ttacaggtac aatagaatca atctcatcaa agaatatgat gcaaggtgaa gattgtcttg
     9481 cccttcgaaa tatctctcgt actccgcgtt ctgactctcc aacccatttt gatagtagct
     9541 caggaccctt tactgaaata aaattggctt cgctttctgt tgctactgct ttagctaaga
     9601 gagtttttcc agttccgctt ggcccataaa gtaaaattcc acgtggcatt gtatggccta
     9661 gcttggagta aagtacaggg tatttcattg gccattctac tgcctcttgc agttcacgtt
     9721 ttacattttc taatcctcct acgtcctccc attttatgtc aggtttttca atgaacacct
     9781 cacgcattcc agctggtgtg acttcacgaa atgccaattg ataatcttct gcatttacaa
     9841 taagcttgtc caaagtttca ggtgggagct tttcatctgc taaattaatt tcaggcagca
     9901 atcttctcaa acatttcatt gcagcttctt tgcagagata ttccaaatct gctcctacat
     9961 atccatggct tattgctgca atcttttcca agtttacatc ttctgttagt ggcatgtttc
    10021 ttgtatgtat caaaagtatg tcatgccttc cctttttgtc tggcactctg atctcaattt
    10081 ctctgtcaaa tcttccaggt cgtctaaggg ctggatccaa tgcgtttggt ctgtttgttg
    10141 ctgcaatgac aattaccttt cctcttcctt ccaaaccatc catgagtgaa agtagttggg
    10201 acaccactct tctctcaact tctcctgtca cctcttctct ttttggagca atggaatcta
    10261 tttcatcgat gaaaacaata gtaggtgact tttctctcgc ttccttgaaa atttctctta
    10321 atcttgctt
//
LOCUS       DATIDL010000017         3067 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrosopumilaceae archaeon isolate SMAG_U16342
            k141_1844525, whole genome shotgun sequence.
ACCESSION   DATIDL010000017 DATIDL010000000
VERSION     DATIDL010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935836
            Sequence Read Archive: SRR25158326
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosopumilaceae archaeon (soil metagenome)
  ORGANISM  Nitrosopumilaceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrosopumilales;
            Nitrosopumilaceae.
REFERENCE   1  (bases 1 to 3067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/04/2024 05:09:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,520
            CDSs (total)                 :: 1,474
            Genes (coding)               :: 1,474
            CDSs (with protein)          :: 1,474
            Genes (RNA)                  :: 46
            rRNAs                        :: 1 (5S)
            complete rRNAs               :: 1 (5S)
            tRNAs                        :: 43
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3067
                     /organism="Nitrosopumilaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1844525"
                     /isolate="SMAG_U16342"
                     /isolation_source="soil"
                     /db_xref="taxon:2202732"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..771)
                     /locus_tag="VLB45_00830"
     CDS             complement(<1..771)
                     /locus_tag="VLB45_00830"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HSD04283.1"
                     /translation="MEPEKLGHKIQKIVKGEVFWDKQYRNIYSVDASSYRIKPLVIAY
                     PKDEHDVISIVKFASKNNISVTARGAGTGLVGSALGNGIILDMRHFDKIKVHSGSVVV
                     GSGVFKGQLDKILEKNDMFFGPNPSVGPYCTVGGMIATNASGSHSLKYGSMIDNLLEI
                     RCVTSDGKIICLPSSAKFSMLGLFESDAREKFPKVSKNSCGYRIDQVTNKSDSQKIIA
                     GSEGTLGIILSAKLKTFPIKRKITLSILAYKTINQAVAD"
     gene            902..1240
                     /gene="mscL"
                     /locus_tag="VLB45_00835"
     CDS             902..1240
                     /gene="mscL"
                     /locus_tag="VLB45_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007550295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HSD04284.1"
                     /translation="MSEPPDVKKSLPKEFMDFLQKFGVIGLAIAFVIGAAASKLVTAL
                     VNDIVTPFIGLAVPDTGDLKAMSFTIGKSTFSYGDLIANLIDFLIIAFVVFLAYKQLS
                     RLKLVEDKKS"
     gene            1290..2414
                     /locus_tag="VLB45_00840"
     CDS             1290..2414
                     /locus_tag="VLB45_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010194473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1512 domain-containing protein"
                     /protein_id="HSD04285.1"
                     /translation="MEFLGINIDELFSSGGDDSNPIMWLVWIVPIIIFMFYGQRIQLQ
                     ITSSEISKSIDKLKKFKEETRTELIDYIKTTIKPATDPTAKLDRFFEHFTIMPVDMDP
                     NGIVPKIRHIMRSREDYVRQQVKALSSDINSIEASKIENILEAATTLHLLYKIVRHLF
                     LTAKKQNNFPLILPLQMMMPFIMEEAEALKTAVSAFKQGQPIGDGIGPMVVGKLMLGI
                     EKKTAAFETVWSESEYEGRKLYLMKAEGPAATVGRPGDALERIISESKPDVMIMVDAA
                     LKMEGEDTGSVAQGFGAAIGGIGTDRFQIEEIATKHKIPTHAIVVKQSIKEAITLMKK
                     EIADSADKVESQIYETIKENTKSGQSVLVIGVGNTLGVSQ"
     gene            2411..2593
                     /locus_tag="VLB45_00845"
     CDS             2411..2593
                     /locus_tag="VLB45_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04286.1"
                     /translation="MIFSKKKEIITPYSLEQCSSCKAISKRKFASGDYIFKNTSKCSS
                     CNGQMSITKIFGEVST"
     gene            complement(2590..>3067)
                     /locus_tag="VLB45_00850"
     CDS             complement(2590..>3067)
                     /locus_tag="VLB45_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007550066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type II methionyl aminopeptidase"
                     /protein_id="HSD04287.1"
                     /translation="IKQMGCIPISNLSGHSLEQYTIHAGKTVPNIWSIGSFSFSPTEA
                     YACEPFVTTREGLGFVREGKTRNIFSIVTRKRTKNAEADKLLDYIWEKFNTLPFALRW
                     LIPEWDEKTARNFLEKLVKSKTIRSYPVLIEANSQRVAQAEHTFIPQENGITITTI"
BASE COUNT          979 a          588 c          590 g          910 t
ORIGIN      
        1 atctgccact gcctgattta ttgtcttgta cgctagtatt gaaagtgtga tttttctttt
       61 aattggaaac gtctttaatt ttgcagataa gatgatgcct agggttcctt ccgaacctgc
      121 aataattttc tgcgagtctg atttgtttgt aacctgatct atcctatatc cgcaagaatt
      181 ttttgatact ttgggaaatt tctctctcgc atctgattca aacaatccca gcatagaaaa
      241 tttagcagat gagggaagac aaattatctt gccatctgag gttacgcatc ttatctccaa
      301 caaattgtca atcatgcttc catactttag agagtggctg ccgcttgcgt ttgttgcaat
      361 cattcctcca acagtgcagt atggtcctac agatggattt ggaccaaaaa acatgtcatt
      421 tttttcaaga attttgtcaa gctgtccctt gaagacacca ctcccaacta caacgctacc
      481 agaatgaacc ttgatcttgt caaaatgtct catgtctaga attatcccat ttcccaatgc
      541 actaccaaca aggcctgttc cagcaccgcg agctgtaaca gaaatattat tttttgatgc
      601 aaattttact atggaaatta catcatgttc gtcttttgga tatgctatga ccagcggttt
      661 tatgcgatag gagctagcat ctacagagta aatattgcga tattgtttgt cccaaaacac
      721 ttcacccttt acaatttttt gaattttatg tcctagtttt tctggctcca tacaagttca
      781 ctctttttat ggcattaaag tttacaatcc ttccaaacac caattgataa actaagcatt
      841 gattgtagtg acaattaact ttcaccatca ttggaatatc tggctatgct atgacaaact
      901 tatgagcgag ccgcctgatg taaaaaagag tctacccaaa gaattcatgg actttctgca
      961 aaaatttgga gtgataggac tagctattgc gtttgtcata ggtgctgcag cttcaaaact
     1021 agttactgcc cttgtaaatg acatagtaac accattcatt ggacttgcag tacctgatac
     1081 cggagatctg aaggcaatgt catttactat aggaaaatca accttctcgt acggtgatct
     1141 catcgcaaat ctcatagact ttctaataat tgcgtttgtg gtgtttttgg cctacaagca
     1201 actttccaga ttaaagttag ttgaagacaa aaaatcctga ttgcctactg acaaagattt
     1261 aatttggcaa aaagcagtgt ttggaattat tggaatttct tggaataaac attgatgagt
     1321 tgtttagcag cggcggtgac gactctaatc ctataatgtg gctcgtatgg atagtgccaa
     1381 taattatttt catgttctat ggacagagaa ttcaactgca aataacatct tctgaaattt
     1441 ctaaatccat agacaaacta aaaaaattca aagaagagac aagaactgaa ctgatagatt
     1501 acataaaaac cacaatcaaa cccgcaactg atccaacagc aaagcttgac agattctttg
     1561 aacattttac aataatgcct gtagacatgg accccaatgg catagtgcca aaaatacgac
     1621 acataatgcg ttcaagagaa gattatgtaa gacagcaagt aaaggcactt tcttctgata
     1681 ttaacagcat tgaagcaagt aaaattgaaa acatactgga agcagctacc actttacatc
     1741 ttttgtacaa aatagtaagg catctgtttc ttactgcaaa aaaacaaaac aactttccac
     1801 ttatattgcc attacaaatg atgatgccat ttatcatgga agaggcagag gcactcaaaa
     1861 ctgcagtgtc tgcattcaag cagggtcagc caatcggtga tggaataggt ccgatggttg
     1921 ttggaaagtt gatgcttgga atcgagaaaa aaactgccgc ctttgagaca gtgtggagcg
     1981 aatcagaata tgagggaaga aaactctatc ttatgaaagc ggaaggaccc gcagcaacag
     2041 ttggaaggcc aggagatgcg cttgaaagaa taatttcaga aagtaaaccc gatgttatga
     2101 taatggttga cgctgcacta aaaatggaag gcgaagatac tggctctgta gctcaaggat
     2161 ttggtgccgc aataggtggc attggtacag acagattcca aatcgaagaa attgcgacaa
     2221 aacacaagat tccaactcac gcaattgtgg taaaacaatc aatcaaagaa gcaataactt
     2281 taatgaaaaa agaaattgca gactctgcag ataaggtaga gtcacaaata tacgaaacaa
     2341 tcaaggaaaa cacaaagagt gggcaatctg tgcttgtaat tggagtggga aacactctag
     2401 gggtatcaca atgatctttt caaagaaaaa agaaatcata actccatatt ctctggaaca
     2461 atgcagttct tgcaaggcaa tatcaaaaag aaaatttgcc agcggagatt atatattcaa
     2521 aaacacgagc aagtgcagtt catgcaacgg acaaatgtca attaccaaaa tttttggcga
     2581 agtttctacc tagattgtag ttatagtgat gccattttct tgtggtataa atgtgtgttc
     2641 agcctgtgcg accctctggc tgtttgcctc aattagtaca ggatatgatc ttatggtctt
     2701 gctttttaca agcttttcca aaaaatttct agctgttttt tcatcccact ctggtatcag
     2761 ccatctcaaa gcaaaaggca aagtgttgaa tttttcccag atgtagtcaa ggagcttgtc
     2821 agcttctgca ttttttgttc gctttcgcgt gacaatagaa aagatgtttc ttgtcttgcc
     2881 ttcgcgcaca aatcctaatc cctcacgggt tgtgacaaat ggctcacacg catatgcttc
     2941 tgtgggagaa aatgagaaag atcctatgga ccagatgttt ggtactgtct ttcctgcatg
     3001 gatagtatat tgttccaatg aatgtccact tagatttgat attgggatgc agcccatctg
     3061 tttgatt
//
LOCUS       DATIDL010000018         5954 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrosopumilaceae archaeon isolate SMAG_U16342
            k141_230982, whole genome shotgun sequence.
ACCESSION   DATIDL010000018 DATIDL010000000
VERSION     DATIDL010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935836
            Sequence Read Archive: SRR25158326
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosopumilaceae archaeon (soil metagenome)
  ORGANISM  Nitrosopumilaceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrosopumilales;
            Nitrosopumilaceae.
REFERENCE   1  (bases 1 to 5954)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5954)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/04/2024 05:09:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,520
            CDSs (total)                 :: 1,474
            Genes (coding)               :: 1,474
            CDSs (with protein)          :: 1,474
            Genes (RNA)                  :: 46
            rRNAs                        :: 1 (5S)
            complete rRNAs               :: 1 (5S)
            tRNAs                        :: 43
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5954
                     /organism="Nitrosopumilaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_230982"
                     /isolate="SMAG_U16342"
                     /isolation_source="soil"
                     /db_xref="taxon:2202732"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..414)
                     /locus_tag="VLB45_00855"
     CDS             complement(<1..414)
                     /locus_tag="VLB45_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanine-N1)-methyltransferase"
                     /protein_id="HSD04288.1"
                     /translation="MQTDQMQDIVEIIEGKTKLLVPKTSLETNAPPMQPAFFNPRAKL
                     SRDFSIVAYSTFLQNFKGPKIFLDCLAGIGARSIRVANELENIEKVFVNDVNPLALEL
                     GRSSAKLNNTEKCEFSENEACRFLSLHSKREERGAI"
     gene            479..1105
                     /gene="rnhB"
                     /locus_tag="VLB45_00860"
     CDS             479..1105
                     /gene="rnhB"
                     /locus_tag="VLB45_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007551235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HSD04289.1"
                     /translation="MIVCGVDDAGRGSVLGPLVIAGVSAERSKIKHLSEIGVKDSKQL
                     SPANRERLYKKIIDIVDDYYVAKISPKLIDKSVSKNRLNHLEAQYMAKVISKLHPDSS
                     YVDSCDVNPARFGKEISNLAQSGKIYSRHHADSRFVIVSAASIVAKVTRDRAIELLRK
                     DHNLGSGYPSDPKTMSFIKKWIVYNKKPPQFVRSSWKPVKLLMKQYCL"
     gene            complement(1102..1815)
                     /locus_tag="VLB45_00865"
     CDS             complement(1102..1815)
                     /locus_tag="VLB45_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrillarin-like rRNA/tRNA
                     2'-O-methyltransferase"
                     /protein_id="HSD04290.1"
                     /translation="MVEAIGRKENEKSLENEEGNIFWVKVDGEKKLATLNLVPGNQVY
                     KEKLVKVKDEEFRLWDPFRSKLAGAIMNGLEFLPIVRKSKVLYLGVSTGTTASHISDI
                     VGPSGIVFCVEHASRVARDFIDRVSSFRSNIVPILQDARQPKQYFSVYGPVDVVYVDI
                     AQPDQTEIAMLNCKTYLKKGGYIMLVIKTRSIDVTKDPIQVMRNEVKKLGPSFEVVQE
                     INLHPYEKDHGIVLAKYLG"
     gene            complement(1757..3070)
                     /locus_tag="VLB45_00870"
     CDS             complement(1757..3070)
                     /locus_tag="VLB45_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HSD04291.1"
                     /translation="MHFVILTELGISVFDKNTCVKSIPFSSAAAQYTEIKEEKADIDQ
                     LQEFLSKINTEIFVNDSSLLKLLKKNSIYAELMEESKIESIQSSKLQVLVDSGFAKDE
                     SDARTKLRDFAVNLSSSRVTEISESPDLHIIQAINALDEIDKIINGLSSRLREWYGLH
                     FPELDNLIDSINGYAKIVLAGNRDSITDSTYTDAGFPDTKVEMLSLLQKKSKGGKISD
                     ENLAIVQVLSKQILEFFDLRNTLEKHVEAQMESIAPNISGILGASVGARVLARAGSLK
                     RLASMPASTIQVLGAEKALFRSLKTGSQPPKHGLLFQHTLVHAAPRWQRGKIARAIAA
                     KAAIAARVDVFGAGKNQMLFEKLNIRIKEIGEKYKEPSEKEPMRVQQRSSFRPREQGR
                     FGRDAGPRRFGRDSGPRRFGGESRNKDGGSDWKKGKRKKFGKRRR"
     gene            3169..4077
                     /locus_tag="VLB45_00875"
     CDS             3169..4077
                     /locus_tag="VLB45_00875"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010189868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HSD04292.1"
                     /translation="MKPDISTEVGAIKLERPTMLASGILGISLEVFARLYKDGAGALV
                     TKSLSKEPWEGYPNPTIVGVKSGYLNAVGLSNPGAPYFAKMISSNTTIPIIVSLVGSV
                     EEDFTFMIKQFENVKLLGYELNLSCPHVEKVGLEVGDDPELVSKIVKSVKAHSKVPVI
                     AKVGLGSSDYLETVRVACESGIDAITAINTVRAMGIDVETTRPLLSHKIGGLSGPAIK
                     PIAVRCVYEISSKFDIPIIGCGGVSDWEDAVEFMLAGASAVQIGSAIGDKWTGVFSEI
                     NQGISKYMEKKGFSKIKEMVGIAKKF"
     gene            4061..4873
                     /locus_tag="VLB45_00880"
     CDS             4061..4873
                     /locus_tag="VLB45_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HSD04293.1"
                     /translation="MQRSSNTPTVVKIEKVIDETPTVRTLIFTDEVFSNVLPGQFAMV
                     WIPGVNELPMSVMISPEKGKAAFTVRKHGVASTALYDLKAGDYIGMRGPYGNSFTIKQ
                     GKLLLVGGGTGLVPLMRLLTFLKKSDEVTILMGSKTKEEVFFEKMANHILEHNKRQVI
                     AVTEDGTYGKAGLVTEVMEKLLSETKFDAVYTCGPEKMMHKVVQLASAKKIPVQASLE
                     RMMKCGMGMCGSCCVGEVLVCRDGTVFDGNYLLSNKEFGYTHRNKAGILENY"
     gene            4941..>5954
                     /locus_tag="VLB45_00885"
     CDS             4941..>5954
                     /locus_tag="VLB45_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HSD04294.1"
                     /translation="MGHPKIVLTADRTLMSPYRGISLATFFGCAPAVDPHRKKNSFWY
                     YMLKDQVTPRVLFDFICNPTSHHNGIADFAPYGLRKVEAALLRDGFKREDVVVAHPDH
                     VEKFIGPETQVVGTYEMDPLGMGPVTMTFTYGRKQTSYDEFYNRDLHMRINAAKKKNG
                     SNAKVIAGASGTWQYNYDPKKIEEYGLYAVLEGELGGIAPEIDGHAGQFFNYLIDGTF
                     DNMNPFSKNDFKVDIKEFGKGDQKHHGRFIHYWDEPDIDMIPDIVEPSMHGMIEVMRG
                     CGRGCKFCDVTLRPLRYYPPEKVKREIEVNVKKGGQRSAWLHSDDIFVYGLDPRKTKN
                     MAPN"
BASE COUNT         1784 a         1113 c         1238 g         1819 t
ORIGIN      
        1 aatggcacca cgctcttctc tttttgaatg caaactaagg aatcggcatg cttcgttttc
       61 agaaaattca catttttctg tattgtttag ctttgcagaa cttcttccaa gctcaagagc
      121 caatggattt acatcattta caaaaacttt ctctatgttt tctaattcgt ttgcaactct
      181 gatacttcta gcgcctattc ctgctagaca atccagaaaa atttttgggc ccttgaagtt
      241 ttgtaaaaac gttgaatatg caactatgga aaagtctctg cttaattttg cccttggatt
      301 gaaaaaagca ggttgcattg gaggtgcgtt tgtttcaagt gaggttttag gaacaagaag
      361 tttggttttt ccctcaatta tctcgacaat gtcttgcatt tgatcggttt gcaaattggt
      421 tctattatta gtatgtctta aaatgattta atacgtctag tgtaggaaaa acacggaaat
      481 gatagtttgt ggtgttgatg acgcaggaag aggttctgta ttgggaccac ttgtaatcgc
      541 aggagtttca gctgaacgct caaagataaa acatctttct gaaattggag taaaagattc
      601 aaaacagctt agtcctgcta acagggaaag gctgtacaaa aaaattattg acattgttga
      661 tgattattac gttgcaaaaa tctctcctaa attaatagac aagagcgtaa gcaaaaacag
      721 gctaaatcac ttggaagctc aatacatggc caaagtcatt tcaaaactac atcctgattc
      781 ttcttatgtg gattcatgtg atgtgaatcc agctaggttt ggaaaagaaa tttcaaatct
      841 tgctcaaagt ggaaaaatct attcaagaca tcatgctgat agtagatttg tcatagtttc
      901 agcggcatca attgttgcca aagtcacgcg tgatagggca atcgaacttt tacgaaaaga
      961 tcacaacctt ggaagcggat atccgtctga tcctaaaacc atgagtttta ttaaaaaatg
     1021 gattgtctac aacaaaaaac ctccacagtt tgtacgctca agctggaagc cagtcaaatt
     1081 attaatgaaa caatactgtc tctagcctag gtattttgca agtactattc catgatcttt
     1141 ttcataaggg tgtaagttga tttcttgaac aacctcaaaa cttggaccta gtttttttac
     1201 ctcgtttctc attacttgga taggatcttt tgtcacatcg atgcttcgtg tttttatgac
     1261 aagcatgatg tagccgcctt ttttgagata ggttttgcag tttagcattg ctatttctgt
     1321 ttgatctggc tgtgcaatgt cgacataaac cacatcgact ggtccataaa ctgaaaagta
     1381 ctgctttggt tgtcttgcat cctgtaatat gggaactatg tttgatctaa atgacgacac
     1441 tctgtctata aaatcgcgag caactctgct tgcatgttcc acacaaaaca caattccact
     1501 tggaccaact atgtcagaaa tgtggcttgc agtagttcct gtggatactc caagatagag
     1561 aactttggat tttctaacta ttggaagaaa ttccagaccg ttcattattg ctcctgcaag
     1621 cttgcttcta aacggatccc ataatcgaaa ttcttcgtct tttactttga ctagcttttc
     1681 tttgtagact tgatttccag gaacgagatt cagtgttgca agtttttttt cgccgtctac
     1741 tttaacccag aaaatattac cttcttcgtt ttccaaactt ttttcgtttt cctttcttcc
     1801 aatcgcttcc accatcttta tttctagact ccccgccaaa ccttcttggt cctgaatctc
     1861 taccaaacct tcttggtcca gcatctctgc caaatcttcc ttgttcccta ggacggaagc
     1921 tgcttcgttg ctgaactctc atgggttctt tttcgcttgg ctctttgtat ttttctccaa
     1981 tctcctttat ccttatgttc aacttttcaa agagcatttg gttttttcca gctccaaaga
     2041 catcaactct tgctgcaatg gctgctttgg cagctatcgc tctagctatc ttgcctcgtt
     2101 gccatcttgg agcagcatga actagtgtat gttggaaaag cagaccgtgt tttggtggtt
     2161 gagagcctgt cttgagtgaa cggaagagtg ccttttctgc cccaagtact tggatggtac
     2221 ttgcaggcat ggaggcaagt cttttgagac ttccggctct tgccaagact ctagctccta
     2281 ctgatgcacc taatatgcca gaaatgtttg gtgcgattga ttccatttga gcctcgacgt
     2341 gtttttctag agtgtttctt aaatcaaaaa attccagtat ttgtttggaa agaacttgga
     2401 caatagcaag gttctcatca gaaattttgc cacccttgct ctttttttgt aaaagcgata
     2461 acatttctac tttggtgtca ggaaaacctg catctgtata cgtgctgtca gtgatgctgt
     2521 ccctgtttcc tgccaagact atctttgcat atccgtttat gctgtcaatt agattgtcaa
     2581 gctctggaaa atgtaatcca taccattctc taagccttga gctgagtcca tttattatct
     2641 tgtcaatttc atccaaagcg tttattgctt gaattatgtg cagatccgga ctttcagaaa
     2701 tttctgttac tcttgaagaa gaaagattta ctgcaaagtc tcgtaatttt gttcttgcat
     2761 cagactcgtc ttttgcaaag ccagaatcca caagaacttg gagcttggaa gactggatgc
     2821 tttcaatctt ggattcttcc atcagctctg cataaattga attttttttc aaaagtttca
     2881 aaagtgaaga gtcatttaca aaaatttcag tatttatttt tgaaagaaac tcttgtaact
     2941 gatcaatgtc tgctttttct tcttttatct cagtgtattg ggctgctgcg cttgaaaatg
     3001 gaatcgattt aacacatgta tttttatcaa ataccgaaat gcctaactct gttaaaatga
     3061 caaaatgcat acttcacact ttgctacact ctcactaaaa aaggtaacat tccatgatgt
     3121 gagattgcag attaactgtt atatgcgaac aattttttat aatcagaagt gaaacctgac
     3181 atctcgacag aagtaggggc aatcaagttg gaaagaccaa caatgcttgc ttcgggaatt
     3241 cttgggatct cacttgaggt ctttgcccgt ttatataaag acggagcagg tgcactagtc
     3301 acaaaatcac taagcaaaga accatgggaa ggttatccaa atcctacaat agttggagta
     3361 aagtctggtt acctaaacgc tgttggtctt tcaaatccgg gggctccata ttttgcaaag
     3421 atgatctcat ctaacaccac aataccaatc attgtaagct tggttggttc tgtagaagaa
     3481 gactttacct ttatgataaa gcagtttgaa aatgtaaaac tcttgggata tgagcttaat
     3541 ctgtcctgtc cccatgtgga aaaagttgga ctagaagttg gagatgatcc tgagcttgtt
     3601 agtaaaatag taaaatctgt aaaagcgcat tccaaagttc ctgtcattgc taaagttgga
     3661 ctgggatcat ctgactatct tgaaacagtt agggtagctt gtgaatctgg aattgatgca
     3721 ataactgcga taaacacagt gcgagcaatg ggaatcgatg tggaaacaac acgacctctt
     3781 ctcagtcata aaattggtgg actctcaggt cctgcaataa aaccaattgc agtaagatgt
     3841 gtgtatgaaa tctcatcaaa gtttgatatt ccgataattg gttgtggtgg tgtttcagac
     3901 tgggaagatg cagtagagtt tatgcttgct ggtgccagcg cagtacagat tggtagtgca
     3961 atcggtgaca aatggactgg agtgttttca gaaattaatc aaggcatatc caagtatatg
     4021 gaaaagaaag gattctcaaa aataaaggaa atggttggaa ttgcaaagaa gttctaacac
     4081 gccaactgtt gttaaaatag aaaaagtaat agatgaaacg ccaacagtgc ggactttgat
     4141 cttcactgac gaagtgtttt ctaatgtact gccaggacag tttgcaatgg tttggattcc
     4201 tggcgtgaat gaattgccaa tgagcgtgat gatatcgcca gagaaaggca aggcggcatt
     4261 tactgtaaga aaacacggcg tggcatctac tgcactgtat gatctcaagg caggcgacta
     4321 tatcggaatg cgtgggccat atggaaattc atttacaata aaacaaggca agctgttgct
     4381 tgttgggggt ggaacaggac ttgtaccctt gatgcgcctt ttgacgtttt tgaaaaaatc
     4441 agacgaagtt accatattga tgggatcaaa aacaaaagag gaagtgtttt ttgaaaagat
     4501 ggcaaaccac attcttgaac acaacaaacg ccaagttatt gctgtaacag aagatggaac
     4561 atatggaaaa gctggtctag tcacagaagt gatggaaaag ttgctgtctg aaacaaagtt
     4621 tgatgcagta tacacatgtg gacctgaaaa gatgatgcac aaagttgtac agcttgcatc
     4681 tgcaaaaaaa atcccggttc aggcaagcct tgaaagaatg atgaaatgtg gtatgggaat
     4741 gtgtggaagt tgctgtgttg gggaagttct tgtatgtcgt gatggaacag tctttgatgg
     4801 gaattatttg ctctcaaata aagaatttgg atatactcac agaaataaag ctggaatttt
     4861 agaaaattat tgatttatgt gtgtaaaagt gggaaaactt aaaatgattg ggctagagaa
     4921 ccctttttag aactaaaaat atgggccacc caaaaattgt attaacggca gatcgaaccc
     4981 tgatgtctcc ataccgcggg atatctcttg ccaccttctt tggttgcgct cctgcagtag
     5041 atccgcacag gaaaaaaaac agcttttggt actatatgtt aaaagatcaa gtaactccta
     5101 gagtcctctt tgatttcata tgtaatccaa ctagtcacca taacggaata gcggattttg
     5161 ctccatacgg attaagaaaa gtagaagctg cactgttgcg tgacgggttc aaaagggaag
     5221 atgtcgttgt agcacatcct gatcacgtag agaagttcat tggtccagaa acccaagtcg
     5281 ttggaacata cgaaatggac ccgcttggaa tgggacctgt caccatgacc ttcacctatg
     5341 gtagaaaaca aacatcgtat gacgagtttt acaacaggga cctgcacatg cgaatcaatg
     5401 ctgcaaagaa aaagaatggc agcaatgcca aagtgattgc gggtgcatca ggaacatggc
     5461 agtataacta tgatcctaaa aaaatagaag aatatggttt gtatgcagtt ttagaaggcg
     5521 aacttggtgg cattgcacca gaaatcgatg gacatgcggg acaatttttc aattatctaa
     5581 ttgatggcac atttgacaac atgaatccgt tttcaaaaaa tgatttcaaa gttgacatta
     5641 aagaatttgg taaaggcgac caaaaacatc atggaagatt catccattat tgggatgagc
     5701 cagacattga catgattcca gatatagttg agccaagcat gcatggaatg attgaggtaa
     5761 tgcgcggatg cggacgcggc tgcaagtttt gtgatgtcac actacgacca ttgagatact
     5821 atcctccaga aaaagtcaaa agggaaatcg aggtgaatgt caaaaaaggc ggacagcgct
     5881 ctgcttggct gcacagcgat gatattttcg tttacggcct tgatccaaga aaaacaaaga
     5941 acatggctcc aaac
//
LOCUS       DATIDL010000019         3346 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrosopumilaceae archaeon isolate SMAG_U16342
            k141_837369, whole genome shotgun sequence.
ACCESSION   DATIDL010000019 DATIDL010000000
VERSION     DATIDL010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935836
            Sequence Read Archive: SRR25158326
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosopumilaceae archaeon (soil metagenome)
  ORGANISM  Nitrosopumilaceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrosopumilales;
            Nitrosopumilaceae.
REFERENCE   1  (bases 1 to 3346)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3346)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/04/2024 05:09:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,520
            CDSs (total)                 :: 1,474
            Genes (coding)               :: 1,474
            CDSs (with protein)          :: 1,474
            Genes (RNA)                  :: 46
            rRNAs                        :: 1 (5S)
            complete rRNAs               :: 1 (5S)
            tRNAs                        :: 43
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3346
                     /organism="Nitrosopumilaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_837369"
                     /isolate="SMAG_U16342"
                     /isolation_source="soil"
                     /db_xref="taxon:2202732"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1478
                     /locus_tag="VLB45_00890"
     CDS             <1..1478
                     /locus_tag="VLB45_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007401939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HSD04295.1"
                     /translation="WEAMSISIENGASISRSALIKQLVNARYERNDTELSSGRFRIKG
                     DTLDIIPAYSDDVIRISMFGDRIEKISVHNNISLKEKKTISKIKIFPAKHYLVANDVR
                     QAAVNSIKKELEQRLDELKELEKQRLEMRTKFDLEMIEELGYCSGIENYSRHFDGRNP
                     GEPAFCLLDFFGDDFLLVIDESHVTLPQLHGMYKGDYSRKKALIEYGFRLPSAFDNRP
                     LKFEEFEKYIKNTIFVSATPAEYERKKSFQTVEQLIRPTGLVDPQVEVRKTKDQMDNL
                     VSEIKKRVEKDQRVLVTTLTKRMAEDLAEFLSKKQVKVRYLHSEIENLQRTELIRQLR
                     LGEFDVLVGINLLREGLDIPEVSLVAILDADKEGFLRNVTSLIQTFGRAARNVDGSVI
                     MYADTITQSMKLAIAETERRRKKQISYNKKMGITPSTIIKAIPMQSTKLDEIKHMSKH
                     DLIKYAIETETAMKRFAEDLDFERAIEYREKLAQIQKELKK"
     gene            1475..>3346
                     /gene="uvrA"
                     /locus_tag="VLB45_00895"
     CDS             1475..>3346
                     /gene="uvrA"
                     /locus_tag="VLB45_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HSD04296.1"
                     /translation="MNDILSIRGAREHNLKNINIDIPKNKLVVITGLSGSGKSTLAFD
                     TIYAEGQRRYVESLSAYARQFLEMMNKPDLDSIEGLSPAISIQQKTTSKNPRSTVGTI
                     TEIYDYLRLLYARIGIPHCTKCGRKISNQSAESICESILKKSDGKNIIILAPLVRRKK
                     GTYEKLFEQVKKMGYSRMRVDNEIIELNGEIPVLDKQKWHNIEIVVDRIKASKEEKSR
                     LFESIQNALKTTKGTVLISAEEEMLFSQNNACSYCGITIGELEPRTFSFNSPFGACKM
                     CHGLGVKMEFEPDLLIPDKSKSILDGAIIPWSGRFSSFRRQTLRDVGKKFGFDLMTPM
                     NKMNSKQMQVILYGTDEVVQFSYESQTTESKWQYANRFSGVIPSLQRNFAETDSESKR
                     EELSKFMRETPCGTCKGKKLKDEALAVKINSMSIMEVCDLSIDACYNFFQNLKLTETE
                     QYIAKSVLKEIISRLEFLKNVGLNYLTLNRVSATLSGGESQRIRLATQIGSNLTGVLY
                     VLDEPTIGLHQRDNSKLIKTLTKLRDLGNTILVVEHDEEVIRNSDWIIDLGPGAGIHG
                     GSVIFEGTIKEILNNHKSVTGKYLKNHDSIKLAKKTRYQKGKLVVKGAAENNLKHIDV
                     "
BASE COUNT         1275 a          543 c          662 g          866 t
ORIGIN      
        1 attgggaagc aatgtcaatt agtattgaaa atggcgcctc aattagtcgc tctgctttga
       61 taaaacagct tgtcaatgca cgttatgaga gaaatgatac tgaactttca tcaggaagat
      121 ttcgaataaa aggagataca ctagatatca tacctgccta ttctgacgat gtcataagaa
      181 tctccatgtt tggagacagg atagaaaaga tatctgtaca taacaatatc tcacttaaag
      241 agaaaaaaac catttcgaag atcaagatat ttcctgcaaa acactatctt gttgcaaacg
      301 atgttagaca agctgcagta aactcgataa aaaaagaatt ggagcaaaga ctagacgagc
      361 ttaaggaatt agaaaaacaa agactagaaa tgagaacaaa atttgatctt gaaatgatag
      421 aagagcttgg atattgttct ggaattgaaa attattcaag acactttgat ggtagaaatc
      481 ctggagagcc agcattttgc ttgcttgatt tttttggaga tgatttcttg ctagtcatag
      541 atgaatcaca tgtaactctg ccacaactac atggcatgta caaaggagat tattctagaa
      601 agaaggcgct gatagagtat gggtttagac ttccaagtgc ttttgataac agaccattaa
      661 agtttgaaga atttgaaaaa tacataaaaa atacaatctt tgtttctgca actcctgcag
      721 aatatgaacg aaaaaaaagc tttcagacag ttgaacaatt aattcgacct acaggccttg
      781 ttgacccaca agtagaggtc agaaaaacaa aagaccagat ggataatttg gtatctgaaa
      841 tcaaaaagag agtagaaaaa gatcaacgag tacttgttac gacacttaca aagcgcatgg
      901 cagaggacct tgcagaattt ctatcaaaaa aacaagtcaa agtcagatat ttacactctg
      961 agattgaaaa cctgcaaagg acagaattga ttaggcagtt gcggcttggc gagtttgacg
     1021 tactagtagg aataaatctg ttaagggaag ggctagacat acctgaagtt tctcttgttg
     1081 ccattttaga tgcagacaag gaaggatttc ttagaaatgt taccagtctg attcagactt
     1141 ttggtagggc agcaagaaat gtagatggaa gtgtgataat gtatgcagat acaataacgc
     1201 aatcaatgaa acttgctatt gcagaaactg aacgaagacg caaaaaacag atctcttaca
     1261 acaaaaagat gggaataaca ccaagtacaa taatcaaagc tatacctatg cagtcaacca
     1321 aattagatga gataaaacac atgtcaaaac atgatctaat caagtatgca attgaaactg
     1381 aaacagcaat gaagaggttt gcagaagatc ttgattttga gcgcgcaata gaatatcgag
     1441 aaaaattggc gcaaattcaa aaggagttga aaaaatgaat gacatactgt ctatacgtgg
     1501 tgcaagagag cacaacttga aaaatatcaa catagacata ccaaaaaaca aacttgttgt
     1561 cattactggg ctttcagggt ctggaaaatc aactctcgca tttgacacaa tatatgcaga
     1621 aggccaacga aggtatgttg agtcactttc tgcatatgca cgacagtttt tagaaatgat
     1681 gaacaaacca gatctagact caatagaagg attatctcct gcaatttcaa ttcaacagaa
     1741 aactacaagc aaaaatccaa gatcaactgt aggcacaata actgaaatct atgactatct
     1801 caggttactt tatgcacgaa taggcattcc tcattgtaca aagtgtggca gaaaaatatc
     1861 aaatcaatct gccgaatcaa tttgcgaatc aatcctaaaa aagtctgacg gaaaaaatat
     1921 cattatactt gctccacttg ttcgcagaaa aaaaggtacc tatgagaagc tgtttgaaca
     1981 ggtaaagaaa atgggttatt ccagaatgag agtggataat gaaataatcg aattaaatgg
     2041 cgagatacca gttcttgaca agcaaaaatg gcacaacata gagatcgtag tagatagaat
     2101 caaagcatca aaagaagaaa aaagtcgact atttgaatcg attcaaaatg cactaaagac
     2161 tacaaaagga actgttctta tttctgcaga agaagaaatg ttgttttcac aaaacaacgc
     2221 ctgctcatat tgcggtatta cgataggcga attagaaccg agaacatttt cgtttaattc
     2281 tccttttggg gcatgcaaga tgtgtcatgg attaggagtc aaaatggagt ttgagccaga
     2341 tttgctcata ccagataaat caaaatcaat actagatggt gccataattc cttggtcagg
     2401 cagattttca tcgtttagaa gacaaacctt aagggatgtt ggcaaaaagt ttggttttga
     2461 tctcatgacc cctatgaaca agatgaattc aaaacagatg caggtgattt tgtatggtac
     2521 tgatgaagta gtccaatttt cttatgaatc acagacaacc gaatcaaaat ggcaatatgc
     2581 taacaggttt agtggagtca tacctagttt gcagagaaat tttgcagaaa ctgactcgga
     2641 atcaaagaga gaagagctaa gcaaatttat gcgtgaaaca ccgtgtggga cttgtaaagg
     2701 gaagaaacta aaagacgagg cacttgcagt caaaataaat tccatgtcaa taatggaagt
     2761 atgtgatctt tcaattgatg catgttacaa tttttttcaa aatctcaagt tgacagaaac
     2821 tgaacaatac attgccaaat cagtattaaa agaaataatt tcacgactag agtttttgaa
     2881 aaacgtcgga ttgaattatc tgacgctaaa tagagtaagc gccaccctat caggaggaga
     2941 atcacaaaga atcagattag caacacagat tggatccaat cttacaggag tactttatgt
     3001 tctagacgag ccaacgatag gattacatca gcgtgacaat tcaaagctta tcaagacact
     3061 tacaaagctt cgtgatcttg gaaacactat acttgtagtt gaacatgatg aggaagtaat
     3121 tcgaaattca gattggataa tagatttggg tccaggtgct ggaattcatg gtggcagtgt
     3181 gatttttgag gggacaatca aagagattct aaacaaccac aaatctgtaa ctggcaaata
     3241 tctaaaaaat catgactcaa taaaacttgc aaaaaaaaca cgctatcaaa aaggaaaact
     3301 agttgtaaaa ggagctgcag aaaataatct caaacacatc gatgta
//
LOCUS       DATIDL010000020         5797 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrosopumilaceae archaeon isolate SMAG_U16342
            k141_439856, whole genome shotgun sequence.
ACCESSION   DATIDL010000020 DATIDL010000000
VERSION     DATIDL010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935836
            Sequence Read Archive: SRR25158326
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosopumilaceae archaeon (soil metagenome)
  ORGANISM  Nitrosopumilaceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrosopumilales;
            Nitrosopumilaceae.
REFERENCE   1  (bases 1 to 5797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5797)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/04/2024 05:09:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,520
            CDSs (total)                 :: 1,474
            Genes (coding)               :: 1,474
            CDSs (with protein)          :: 1,474
            Genes (RNA)                  :: 46
            rRNAs                        :: 1 (5S)
            complete rRNAs               :: 1 (5S)
            tRNAs                        :: 43
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5797
                     /organism="Nitrosopumilaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_439856"
                     /isolate="SMAG_U16342"
                     /isolation_source="soil"
                     /db_xref="taxon:2202732"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..699)
                     /locus_tag="VLB45_00900"
     CDS             complement(<1..699)
                     /locus_tag="VLB45_00900"
                     /inference="COORDINATES: protein motif:HMM:NF042804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6775 family putative metallopeptidase"
                     /protein_id="HSD04297.1"
                     /translation="MKISTVYLYDEPSVPEIKLKELGEFIKNNFSFNVEIRESIFSFF
                     KDANDQIAYDLAACRIFDPKRPFERHVPTSEEVVFEKESFFHSKETNIIMYDGFEFQK
                     ILTDIIPEEERHADRFHMVCTNKLTCTFDYDDYRYHGRAVICSNPAIISTTGMIEAPA
                     KPREYYLNLLTNMIQGLNVDAIKNQFVGTHLQYHDPKLSEIIKGYAMQALFYYLTGEP
                     FCESKECRLFNAHWQ"
     gene            complement(723..1541)
                     /locus_tag="VLB45_00905"
     CDS             complement(723..1541)
                     /locus_tag="VLB45_00905"
                     /EC_number="2.4.2.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide-dependent adenosine diphosphate thiazole
                     synthase"
                     /protein_id="HSD04298.1"
                     /translation="MQQAEYTNEVPKIFADVKEMEITRAIANEFHEVLINGADSDIIV
                     IGAGPAGLTASRELAMMGFKTLVIEQNNYLGGGYWLGGYMMNPVTVRAPAQKIWDELG
                     IPYKKVSEGLYLTPGPHAVSKLIAAACDAGVKFLNLTKFDDLVLKNGRVSGVVVNWMP
                     VSALPRNITCVDPVALESKMVIDASGHDSVAVKRLVDRKLVEWKGMNPMWVDDGENRV
                     VHKTGEVFPGLVVAGMSVTETFGIARMGPTYGSMLLSGKKAAEVTAAKMKELRK"
     gene            complement(1579..2586)
                     /locus_tag="VLB45_00910"
     CDS             complement(1579..2586)
                     /locus_tag="VLB45_00910"
                     /inference="COORDINATES: protein motif:HMM:NF013795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04299.1"
                     /translation="MPDKISIVLIGFGNVTKTLVKGLQFYKNSTDGLWHPKLAGFTLN
                     DITINGIFDVDSTKVGKKVRDLITDQKVDSDLSIQAGVFDDPVNVLGSEIKSVKQNEF
                     VESLKKLKPDFVINVISSGMDKSGESYAKAALEAGCSYFNATSSKIITPQLKSSFESK
                     GLMILGDDLMSQFGGTSFHRGMIEFMVNRGITIQKAYQLDVGGNPDTQNTMAELLREK
                     KRKIKTESISIESPYPFKSTAGTTEYAAQLGNSRVSYYWMEARGFLGAPIEMDLSLRT
                     NDSSNGCNVILDSIRAAKSSIAKKDFAKADVISAYAFKNPLKKTKIRESIRAFEEAFS
                     N"
     gene            2774..4579
                     /locus_tag="VLB45_00915"
     CDS             2774..4579
                     /locus_tag="VLB45_00915"
                     /EC_number="1.8.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HSD04300.1"
                     /translation="MSKIRLSQSKPDNLPKPKINWGRLEEADKFAETVKLFRQGWINS
                     DDFRRFRLQHGAYGSRMTDNYSMVRIKIPAGEIYPEQIEKIAQLSESFSIGSAHVSTR
                     QNFQLHWVFLEDVSEIMRGLADVGMTSREACGNTVRTVMCSPLAGVCDEEIFDPTPYA
                     LAVSKFLLRNPLNQSLPRKFKINFTCCEKHGMARIADIGLIPQKSEKDGKTQNGFKVF
                     LGGGLGAQSFVGHHLEEFTPEENLLYTVISVLRIFDRMGNRENMARNRMRYLVNEMGW
                     QKFQNLVLKERAIVRSTQSVVVKLDVDKTPEQLHKMIVSSDNPSTPNGFSRWVKSNVI
                     KQKQSGYSAVFLTLESGDVTANQLRAIAQMAREFSAEGRARNGFTQDIVFRWVRDEDL
                     PQLYSKLLEVGLANPGALTIASVVGCSGTTSCNLALTNSHRLAKELQRKFLELKLDTD
                     DDLRDSSIKISGCPNSCGQHEISTIGFFGGGARINKNMYPLYTMSLGGRADEQAMLGL
                     YCMRVPAKRVIPVILKIIEIFKSEKKQGETLNSWIHRVIDGYGGPNIKSLNDIKEKLK
                     PIVAAPSIEEDKDFYVDYGSDGGYHAKTGRGECAA"
     gene            4567..5379
                     /locus_tag="VLB45_00920"
     CDS             4567..5379
                     /locus_tag="VLB45_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007401388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HSD04301.1"
                     /translation="MCCLSTEKSMHITIDADSLRSQIRDKSVRVLDIRKEDDYKQNHI
                     PGAVNLPLANLLADDSPQKVLQLTQSFGIDDETPVVVYDDTFGALASRVAWTLQYIGH
                     DDVSLLDVTYGTWKKLGLETSTEQTSVNKKTHSLKLKPEILATVDYLESAKEKKETIL
                     IDNRERLNYLDNHIPGAINIPYRMLASADSILRPKEELKRLVQNRNISSRSEIITYCG
                     SVGTLSGLGYYALKSIGMENVKLYVRSFKEWKSLEKPVEVQKDANYWDLSAE"
     gene            complement(5383..>5797)
                     /locus_tag="VLB45_00925"
     CDS             complement(5383..>5797)
                     /locus_tag="VLB45_00925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04302.1"
                     /translation="EKKQGFHIQEFYDIAPEIAEKMGVERKAKLGITKKSAASIQHLK
                     EISEAIQDAKSSIEKSDVEQTLANLDVFIEPSKKGPQMIEYFFEEHREIRLHRIRIKD
                     RGIEYLQANKQKILEFFDSMEANITKKLRSTVAQN"
BASE COUNT         1758 a         1127 c         1093 g         1819 t
ORIGIN      
        1 ttgccaatgc gcattaaaca atctgcactc ttttgattca caaaacggtt ctcctgttag
       61 ataataaaac aatgcttgca ttgcataacc tttgatgatt tcagaaagtt tgggatcgtg
      121 atactgaaga tgagtgccca caaattggtt tttgattgca tcaacattca atccttgtat
      181 catgtttgtt agcaaattta gataatactc tcgtggtttt gctggcgcct caatcattcc
      241 tgtggtggaa ataattgcag gatttgaaca aattaccgct ctgccatgat atctgtaatc
      301 gtcataatca aacgtgcaag taagtttgtt tgtgcaaacc atgtggaatc tgtctgcatg
      361 tctctcttct tcaggtatta tatcggtcag gattttttga aactcaaatc catcatacat
      421 tatgatgttg gtctctttcg aatgaaaaaa tgactctttt tcaaaaacta cctcttcact
      481 agttgggacg tgcctttcaa atggtctttt tggatcaaaa attctacacg ctgccagatc
      541 gtaagcaatt tggtcatttg catctttaaa gaatgaaaaa atactttccc taatttctac
      601 attaaaagaa aaattatttt ttataaactc tccaagttct tttaatttta tttctggaac
      661 ggatggttca tcataaagat agactgtaga aattttcaat gattcaacaa tgggttactg
      721 gtttattttc ttagttcttt catctttgca gcagtaactt ctgctgcctt tttgcctgaa
      781 aggagcatcg agccatatgt cggtcccatc cttgcaattc caaaagtctc tgttactgac
      841 attccagcta caacaagacc gggaaacact tcgccggttt tgtgaaccac tctgttttct
      901 ccgtcgtcta cccacattgg gttcatgcct ttccactcca ctaattttct gtctaccagt
      961 ctttttaccg caacagaatc gtgaccagaa gcgtcaatta ccatctttga ttccagtgct
     1021 acaggatcta cacaagtaat gtttcttggt aatgccgaaa ctggcatcca gtttacaaca
     1081 actcctgaaa ctctgccgtt ttttaaaacc aaatcatcaa attttgttaa attaagaaac
     1141 tttacgcctg catcacatgc agctgcaata agctttgata ctgcatgagg tccaggggta
     1201 aggtacagac cctctgaaac ttttttgtat ggtatgccaa gctcatccca aattttttgt
     1261 gctggtgctc tcacagtaac tggattcatc atgtatcctc caagccagta tcctccacca
     1321 agatagttgt tttgctcaat taccaaagtc ttgaaaccca tcattgcaag ctctctgctt
     1381 gcagtaaggc ctgcagggcc agctcctatg actatgatgt cagaatctgc gccattgatt
     1441 aaaacttcat gaaattcgtt agcaatagct cttgtaattt ccatctcttt aacatctgca
     1501 aaaatttttg gtacttcgtt agtgtattca gcttgctgca tgcaaattca tactaaaata
     1561 agtacattaa tagtttgctc agttagaaaa tgcttcttca aacgctctaa ttgattctct
     1621 gatctttgtt ttctttaatg gatttttaaa agcatacgca gagatgacat ctgctttagc
     1681 aaagtctttt tttgcaattg atgattttgc agcccttatg gaatcaagta tcacattaca
     1741 accatttgag ctatcatttg ttctaaggga caaatccatt tctattggtg ccccaagaaa
     1801 tcctctggct tccatccagt agtaactcac tctagaattt ccaagttgcg ctgcatattc
     1861 tgtggttcct gcagttgatt tgaaaggata cggagattcg attgatattg attctgtttt
     1921 tatttttctc ttcttctctc ttagcagctc tgccattgtg ttttgtgtat caggatttcc
     1981 gcctacatca agttgatatg ctttctgtat tgttatgccc ctgtttacca tgaattcaat
     2041 cattcctcga tgaaacgagg ttcctccaaa ctgactcatc aagtcatctc caagaatcat
     2101 caggcctttg gactcaaatg aagactttag ctgtggagtt atgatctttg atgatgtcgc
     2161 attaaaataa gaacaaccgg cttccagagc tgctttggca taactctcac cactcttgtc
     2221 catgccagag gatattacat ttatgacaaa atctggtttt agtttcttca aagattctac
     2281 aaactcattt tgttttacag attttatctc gctgcccaaa acatttacag gatcatcaaa
     2341 gactccagct tgtattgata gatcagaatc tactttctgg tctgttatga ggtctctgac
     2401 tttttttccc actttggtgg aatccacatc aaaaatacca tttattgtaa tgtcatttaa
     2461 cgtaaaacct gcaagttttg gatgccatag tccatcagtt gaatttttgt aaaactgtaa
     2521 tcccttgact agtgtttttg tgacattccc aaatccaatt aaaactatgc tgatcttatc
     2581 tggcatgtga tttttttctt gccggtgatc atttaactct tttccagata gtttagttag
     2641 ctcaaactaa gactgattca aaacatgacg tgactcacac aatacagtat ttgcccagct
     2701 aacttgttgc atttagctaa aattccacta ttcaaaaatt tatatgcctt ccaccgagaa
     2761 attcattcgt acgttgagta agattcgttt aagccaatct aaacctgata atttacctaa
     2821 accaaagatc aactggggta gactagagga agctgacaaa tttgcagaaa ccgtgaagct
     2881 tttcagacaa ggttggataa actctgatga ttttaggcgt ttcagattac aacatggtgc
     2941 ttacggaagc aggatgacag ataattatag catggtcagg ataaaaattc cagctggtga
     3001 aatctatcca gaacaaattg aaaaaattgc acaactaagc gaatcattct ctattggaag
     3061 tgcacatgtg tcaacaagac aaaactttca attgcactgg gtctttttag aagatgtttc
     3121 tgaaatcatg cgcggtttag cagacgtggg tatgacctct agagaagcat gtggaaacac
     3181 tgtaagaact gtgatgtgca gtccgcttgc aggtgtgtgt gatgaagaaa tttttgatcc
     3241 aactccatac gcacttgcag tttcaaaatt tcttttaaga aatccactaa accaatctct
     3301 gccaagaaag ttcaagatca atttcacatg ttgtgaaaaa catggaatgg caagaatagc
     3361 tgatattggt ctaatcccac aaaagtcaga aaaggacggc aagactcaaa atggattcaa
     3421 agtctttttg ggcggtggat tgggcgcaca atcatttgtt gggcaccacc tagaggaatt
     3481 cacaccagaa gaaaatctgc tatacacggt catctctgtt ttgcgaatct ttgatagaat
     3541 gggtaatcga gaaaacatgg caagaaacag aatgagatat cttgttaatg agatgggatg
     3601 gcagaaattc caaaatcttg tactaaaaga aagagcaatc gtacgctcta cccagtctgt
     3661 tgtggtgaaa ctagatgtgg acaagactcc tgaacaatta cacaaaatga ttgttagctc
     3721 agacaaccct tccactccaa atggattttc aagatgggtc aaaagcaacg tcataaagca
     3781 aaaacaaagt gggtacagtg cggtgtttct gactttggag tctggcgatg taactgcaaa
     3841 ccaactacga gcaatagcgc aaatggcaag agagttttca gcagaagggc gagcccgaaa
     3901 tggattcacg caagatattg tgtttaggtg ggtaagagac gaagatcttc cacaacttta
     3961 ttcaaaattg ttagaagttg gtcttgcaaa tccgggtgcc ctgacaatag cgtcagtggt
     4021 gggatgttct ggaacaactt catgtaatct ggctttgaca aactcgcaca gacttgcaaa
     4081 agagcttcaa agaaaattct tggaactcaa gcttgatact gatgatgacc taagggattc
     4141 tagtataaag ataagtggat gtccaaattc ttgtggccaa catgaaatat ctacaattgg
     4201 attttttggt ggtggggcac gtataaacaa aaacatgtat ccactttata ccatgtctct
     4261 tggtggcaga gcagatgaac aagcaatgtt gggattgtat tgcatgagag tgccagcaaa
     4321 aagagtgatt ccagtaatct taaaaataat tgagatattc aagtctgaga aaaaacaagg
     4381 tgagactcta aactcatgga tacaccgtgt tatcgacgga tatggtggac caaacataaa
     4441 atcattaaat gatatcaaag aaaaactaaa gccaatagtg gcagctcctt caatagaaga
     4501 ggacaaggat ttctatgttg actatggaag cgacggtgga tatcatgcaa aaacaggcag
     4561 gggagaatgt gctgcttgag tacagaaaaa tcgatgcata ttacaataga tgcggattca
     4621 ttacgatccc aaattagaga caagagcgtt agagtactgg acatcagaaa agaggatgat
     4681 tacaaacaga accatatccc aggcgcagtc aatctgcctc tagcaaatct tcttgcagat
     4741 gatagtccac aaaaagtctt acagctcaca caatcgtttg gtatagatga tgaaactcct
     4801 gtcgttgtct atgatgacac atttggtgca ctggcatcaa gggttgcatg gacattacaa
     4861 tacattggtc atgatgacgt atctttgtta gatgttacgt atggaacatg gaagaagctt
     4921 ggtcttgaaa caagtaccga acaaacttct gtcaataaaa aaacccactc actaaaactc
     4981 aaaccagaaa ttcttgccac tgtagattat ttggaaagcg caaaagaaaa aaaagaaacc
     5041 atactgattg acaacagaga acgattaaac tatcttgata atcacatccc aggcgctatc
     5101 aacattccat ataggatgct tgcttctgct gatagcattt tgagaccaaa agaggagcta
     5161 aaacgtctag tccagaacag aaacatctcg tcacgctctg aaatcataac ctattgcggt
     5221 agtgtaggga ctctttctgg attgggatac tatgcgttaa aatcgatagg catggaaaat
     5281 gtgaaactat acgtgcgttc atttaaggaa tggaaatcgc ttgaaaaacc agttgaggtg
     5341 caaaaagatg caaattactg ggatctttca gcagaataaa atctaattct gagcaacagt
     5401 gcttcgaagc ttctttgtaa tgtttgcttc catgctgtca aagaattcta gtattttttg
     5461 cttgtttgct tgtagatatt caattcctct atcctttatt ctgattctgt gtaatctgat
     5521 ttctctgtgc tcttcaaaaa agtattcaat catctgagga cctttctttg aaggttctat
     5581 aaagacatca agatttgcaa gtgtttgctc aacatctgat ttttctattg atgattttgc
     5641 atcttgtatt gcttcactaa tttctttaag gtgttgtatg gatgctgcgc ttttctttgt
     5701 gattccaagt tttgcttttc tttcaacgcc catcttttct gcaatctctg gagcaatgtc
     5761 ataaaattct tggatgtgaa atccttgctt tttttct
//
LOCUS       DATIDL010000021         5279 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrosopumilaceae archaeon isolate SMAG_U16342
            k141_2054076, whole genome shotgun sequence.
ACCESSION   DATIDL010000021 DATIDL010000000
VERSION     DATIDL010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935836
            Sequence Read Archive: SRR25158326
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosopumilaceae archaeon (soil metagenome)
  ORGANISM  Nitrosopumilaceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrosopumilales;
            Nitrosopumilaceae.
REFERENCE   1  (bases 1 to 5279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/04/2024 05:09:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,520
            CDSs (total)                 :: 1,474
            Genes (coding)               :: 1,474
            CDSs (with protein)          :: 1,474
            Genes (RNA)                  :: 46
            rRNAs                        :: 1 (5S)
            complete rRNAs               :: 1 (5S)
            tRNAs                        :: 43
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5279
                     /organism="Nitrosopumilaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2054076"
                     /isolate="SMAG_U16342"
                     /isolation_source="soil"
                     /db_xref="taxon:2202732"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..547
                     /gene="rplC"
                     /locus_tag="VLB45_00930"
     CDS             <1..547
                     /gene="rplC"
                     /locus_tag="VLB45_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010194288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HSD04303.1"
                     /translation="AGREQKKPYVFEVAVKGGDITKQFAYVKDLLGKEVKIDQVFQKG
                     SDVDVAAITKGKGIEGPITRWGVKKKQHKSRKSVRALGTLGPISPATIMYTVPRAGQR
                     GFHQRTQYNNRIMIIGNTQNNEYKINPAGGFKHFGLVDGDYVVIRGSIPGTYRRLVKL
                     RAPMRHRVSKVTAPNILEIMV"
     gene            544..1359
                     /gene="rplD"
                     /locus_tag="VLB45_00935"
     CDS             544..1359
                     /gene="rplD"
                     /locus_tag="VLB45_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HSD04304.1"
                     /translation="MKAQVFSIDGSKSDQVELPLVFSTPLRTDLLHKAYVNLESHQFQ
                     IQGRYANAGMDVVAESNSPPTGHHQARVARMHGGGGGRQGQGGGVAMVRGGRQAHPPT
                     TAKVIHKLLNKKENRLALCSAIAATASADLVKSRGHKVDKLESFPIVVSDEIESISKA
                     KDLAKFFDALNLTQDVERLKSRLKRRSGKSLLRGRNTKIGKSILIVVKDSKNLSKATG
                     TFLGVDVKSVDSLSVLDLVPGSQPIRLTVFSKNAIDEIAKIKSTHLEIMVNTK"
     gene            1356..1622
                     /gene="rplW"
                     /locus_tag="VLB45_00940"
     CDS             1356..1622
                     /gene="rplW"
                     /locus_tag="VLB45_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008301481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HSD04305.1"
                     /translation="MNTDEAIKVVLKPYITEKTFALVEKQSQICFIVKDTATKPKIME
                     AVKILYNEEPLAVNTCRSVYGKKAFVKFQTVEKARDLATKIGML"
     gene            1664..2059
                     /locus_tag="VLB45_00945"
     CDS             1664..2059
                     /locus_tag="VLB45_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HSD04306.1"
                     /translation="MVKEFKYRGVTTEELQNLSLEKLFELLPARSRRSLTRGITDGKR
                     KLLEEVKASKTGKSTNPIKTHLRDLIILPYMIGVTVQVFTGKEFHSVTVKPEMVGHYL
                     GEYALTNKRVVHGAPGVGASRSSLYVPLK"
     gene            2065..2523
                     /locus_tag="VLB45_00950"
     CDS             2065..2523
                     /locus_tag="VLB45_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HSD04307.1"
                     /translation="MVHFKYSFQNYDKTKHVRSSLREKTISHKHAREIAVTIKGMTIE
                     KARNYLDDVVRLKRAVPFRRYHNEVGHKSDTGVMSGRYPQKAAGEFIKLIDNLESNAE
                     YKGMDLDRLRIVNATVHKGRKIQRFTPRAMGRASPKIDILTHVELVAQEI"
     gene            2525..3289
                     /locus_tag="VLB45_00955"
     CDS             2525..3289
                     /locus_tag="VLB45_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HSD04308.1"
                     /translation="MSSVKNVINDSYKLMLLKDYLREQIKEAGFDEVDIQKTPTGTRV
                     ALHVTRPGIVIGRKGSGIRDLTQALEKKFDLKNPQISVVEIVKPELYPSVMCNRMAQH
                     LARGTAFRRAVMWTMQTIMNAGAMGVQITISGKLRGERSAFEKHSKGILPRAGHHANV
                     VVSEDIVHVDTPMGLIGVRIRIARKENYIPEFELKATKKEIPRPQVKVIEIDEKAPIE
                     IPIDPTKPKTESEVIEQEEKIIETIETYDEVEEELK"
     gene            3289..3492
                     /gene="rpmC"
                     /locus_tag="VLB45_00960"
     CDS             3289..3492
                     /gene="rpmC"
                     /locus_tag="VLB45_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007401547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HSD04309.1"
                     /translation="MTRLKMKTIREFNETDLKDRLGQLRSDLAKLRVEASKGTLRKEN
                     GKVRSLRRDIARVLTRLGEMKKQ"
     gene            3489..3758
                     /locus_tag="VLB45_00965"
     CDS             3489..3758
                     /locus_tag="VLB45_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030677 - ribonuclease P complex
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0001682 - tRNA 5'-leader removal [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein subunit"
                     /protein_id="HSD04310.1"
                     /translation="MITPENIMMHELIGLHTTIVESNNSGITGMTGKVIDETKSMIIL
                     DTEKGIKKIAKENTQWKFSLGTVETLVSGNMLTKRPQERIGGRHG"
     gene            3751..4074
                     /locus_tag="VLB45_00970"
     CDS             3751..4074
                     /locus_tag="VLB45_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HSD04311.1"
                     /translation="MARNIGISVHAPKTECNDHFCPFHGRLSVRGKLIEGKVVSAKAP
                     KMIVVQQESHKFITKFKRYARSQSKVHAYKPSCIDLKEGDTVLTAECRPISKSVSFVV
                     VEVMS"
     gene            4074..4511
                     /locus_tag="VLB45_00975"
     CDS             4074..4511
                     /locus_tag="VLB45_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HSD04312.1"
                     /translation="MGATKSRAVSAKGVQEFRPYITRALPLGAQVVCADNTGAKILEI
                     VMVQKTKTRVSRYPSAAVGDFVNVVVKKGPGELRKQIYGAVIIRQKYVIKRLSGVRVS
                     FEDNAAVLVTPEGEIKGTEIKGPVAVEASEKWPRIASLASMVV"
     gene            4513..5049
                     /gene="rplX"
                     /locus_tag="VLB45_00980"
     CDS             4513..5049
                     /gene="rplX"
                     /locus_tag="VLB45_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HSD04313.1"
                     /translation="MKPTIVRNRQIFQAANHTKSRQLGATLSKELREKYGARNTRVIK
                     GDTVHVMRGEFKGIDGKVTRVSTEKNSVAVEGIKREKLKGGNIDIFIHTSNVMITDMN
                     LDDKWRQNRLAGQATKTPTEKPKEESKEKPKPQEKVKTIKEKPKAEKETPKEKTVKSE
                     EKKRKQTKAVKPAKKENK"
     gene            5049..>5279
                     /locus_tag="VLB45_00985"
     CDS             5049..>5279
                     /locus_tag="VLB45_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4e"
                     /protein_id="HSD04314.1"
                     /translation="MVSIAGSKKLKRQMAPVFWGITRKDSRFVVTVRPGGHSKHLSIP
                     SAVFLRDTLKLVTTLREAKSAIYGGKVKVDGVT"
BASE COUNT         2008 a          858 c         1122 g         1291 t
ORIGIN      
        1 agctggtagg gagcaaaaaa aaccatatgt ttttgaagtt gctgtaaaag gtggcgatat
       61 tacaaaacaa ttcgcatatg taaaagatct cttaggtaaa gaagttaaaa ttgatcaagt
      121 ttttcaaaag ggctctgatg ttgatgttgc tgccataaca aaaggaaaag gaattgaagg
      181 accaatcaca agatggggtg ttaaaaagaa gcaacacaaa tcgagaaaaa gtgtaagagc
      241 tcttggtaca cttggaccaa tcagtccagc aacgatcatg tacaccgttc caagagctgg
      301 tcagcgtggg tttcatcaaa ggactcaata caataataga attatgataa taggtaatac
      361 tcaaaataat gaatacaaaa tcaatccggc tggaggattc aaacattttg gattggtcga
      421 tggagattat gttgtaattc gcggttcaat tcctggaact tatcgtagac tagtaaaact
      481 gcgagctcca atgcgtcacc gagtttctaa agtaacggca cctaacattt tggagatcat
      541 ggtatgaaag ctcaagtgtt ctcaatagat ggttcaaaat ctgatcaggt ggagttgcca
      601 ttggtatttt ctactccact tagaactgat cttttgcata aagcgtacgt gaatttagaa
      661 tcgcatcaat tccaaatcca aggtaggtat gcaaatgcag gtatggatgt tgttgctgaa
      721 tcaaatagtc cacctactgg tcatcatcaa gcaagagttg caagaatgca tggtggtgga
      781 ggtggaagac aaggtcaagg tggtggtgtt gctatggtaa gaggtggaag acaagcacat
      841 cctcctacaa cagccaaagt aatccacaaa ttgctaaata aaaaagaaaa tagacttgca
      901 ctatgttctg caatagcagc aactgcgtca gcagatcttg tgaaatcacg tggtcacaaa
      961 gtagacaaac tagaatcatt tccaattgtt gtgtccgatg aaatcgaatc aatatctaaa
     1021 gcaaaagacc ttgccaaatt ctttgatgca ttaaatttga cacaggatgt tgaaagactg
     1081 aaatcaagac taaaacgacg ttctggaaaa tcccttttgc gtggcagaaa tactaagatt
     1141 ggaaaaagca tccttattgt tgtaaaagat tcaaagaatt tatctaaagc aactggaaca
     1201 tttttaggag ttgacgtaaa atcagttgat agcctgagtg ttttagatct tgttcctggc
     1261 tcacaaccaa ttagattaac agtattttca aaaaatgcaa tagatgaaat tgcaaagatc
     1321 aaatcaacac atctggagat aatggtgaac acaaaatgaa tactgatgaa gctatcaaag
     1381 tagtactaaa gccgtacatt acagaaaaaa catttgctct agttgaaaaa caaagtcaga
     1441 tttgttttat tgtaaaggac actgccacaa aacccaagat tatggaggca gtaaagattc
     1501 tctacaacga agaaccactg gcagtaaata catgcagatc agtatatgga aaaaaggcat
     1561 ttgttaaatt ccaaactgtt gaaaaagcac gtgatcttgc aacaaagata ggaatgctat
     1621 aatatggtag gcattgaact ctctcgaata caggtgaatt tgattggtta aagaattcaa
     1681 atatcgtgga gtaacaacag aggaacttca gaatctttca ctggaaaaac tgttcgagct
     1741 tttgccagcc agatctagaa gatctttgac tagaggaatt actgatggaa aacgaaaatt
     1801 gctagaagaa gtcaaagcaa gcaaaacagg aaaatcgaca aatccaatca aaactcatct
     1861 tagggatcta attattctgc cttatatgat tggagttaca gtccaagttt ttactggtaa
     1921 agaatttcat tcagttactg tgaaacccga gatggtagga cattacttgg gagaatacgc
     1981 attaacaaac aaaagagtag ttcatggagc ccctggtgta ggagcatcaa ggtcaagctt
     2041 gtatgtaccg ctaaagtgat tgacatggtt catttcaaat attcttttca aaattatgat
     2101 aagacaaaac atgtacgttc ttctctaaga gaaaagacaa tttcgcacaa acatgcgcga
     2161 gaaattgctg ttaccataaa gggaatgaca attgaaaaag cacgaaacta cttagatgat
     2221 gttgtaagac tgaaacgtgc agttccattt agaagatatc ataacgaagt tggacacaaa
     2281 tccgatactg gtgtaatgtc tggtaggtat ccacaaaaag cagcaggtga attcatcaaa
     2341 cttattgata atttggaatc aaatgcagaa tacaaaggaa tggatttgga tagattgcgc
     2401 atagtaaacg cgactgttca caaggggaga aaaattcaaa gatttactcc gcgtgcaatg
     2461 ggaagagcat ctccaaaaat tgacatactt acacatgtag agctagttgc tcaggagatc
     2521 taaaatgtcg tcggtaaaaa atgtcatcaa tgatagctac aaactaatgt tactcaaaga
     2581 ttatttgcgt gaacaaatta aagaggctgg ttttgatgaa gtcgacatac aaaaaactcc
     2641 aactggaaca cgtgtcgcat tgcatgtcac cagaccagga attgtaattg gcagaaaagg
     2701 ttctggcata cgagatctta ctcaagccct agaaaagaag tttgatctta agaatccaca
     2761 aatttcagta gtagaaatcg taaagcccga attgtatcct agtgtaatgt gcaacagaat
     2821 ggcacagcat cttgcaagag gaactgcatt tagacgtgca gtcatgtgga cgatgcaaac
     2881 aataatgaat gcaggtgcaa tgggtgtaca aataactatc tcaggaaagc taaggggaga
     2941 acgttcggct tttgaaaaac acagtaaggg aatactgcct cgtgcaggac accatgcaaa
     3001 tgttgttgtt tcagaagata ttgtacatgt agatactcca atggggttaa tcggagttag
     3061 aattagaatt gcaagaaaag aaaactacat tccagaattt gaactcaagg caacaaagaa
     3121 agaaattccg cgtcctcagg taaaagtaat cgaaatagat gaaaaagctc caatagaaat
     3181 accgatagac ccaactaaac caaaaactga aagcgaggta attgaacaag aagaaaaaat
     3241 aatcgaaact atagagactt atgatgaagt tgaagaggag ctaaagtaat gactcgacta
     3301 aagatgaaaa caatacgtga atttaatgaa acagatctta aagatagact aggacaatta
     3361 cgttcagatc ttgcgaagct acgtgtagaa gcatctaaag gaacactacg aaaagaaaat
     3421 ggtaaggtca gatcactgcg aagagatatt gcacgggttc tgacaagact aggtgagatg
     3481 aaaaaacaat gatcacgcca gaaaacatca tgatgcatga attaattgga cttcatacta
     3541 ctattgttga atctaataat tctggaatca cggggatgac tggaaaagta atcgatgaaa
     3601 caaaatcgat gattatcctt gacactgaaa agggaattaa aaaaattgca aaagaaaata
     3661 cacaatggaa gttttctctt ggtactgtag aaactttggt atctggtaac atgttgacaa
     3721 aaagacctca ggaaagaata ggaggaagac atggctagaa acataggtat ttcagtacat
     3781 gctccaaaga ctgaatgcaa tgatcatttc tgtccttttc atggtagatt gagtgttaga
     3841 ggaaaattga ttgaaggaaa agtagttagt gcaaaggctc caaaaatgat tgtagtccaa
     3901 caggaatcac acaaattcat tacgaaattc aaaagatatg cacgtagcca aagtaaagtt
     3961 catgcataca aaccatcatg cattgatcta aaagaaggcg atactgttct aactgcagag
     4021 tgtagaccga tatcaaagag tgtttcattt gtggtagtag aggtcatgtc ataatgggcg
     4081 caacaaagag ccgtgcagta tctgcaaaag gagttcagga attccgccca tacattactc
     4141 gagcacttcc acttggcgct caagtagtat gtgcagataa tacgggtgca aaaattttgg
     4201 aaattgtaat ggtacaaaaa acaaagacta gagtttctag atatccttct gcagcagttg
     4261 gtgattttgt taatgtcgta gtaaagaaag gaccaggaga gcttagaaag caaatctatg
     4321 gggcagtaat tatacgacaa aaatatgtaa tcaaaagact aagcggtgta cgagtttcat
     4381 ttgaagataa tgcagctgtg cttgttactc ctgaaggaga aatcaagggt accgaaatca
     4441 aaggcccagt agctgttgaa gcatcagaaa aatggccaag aatagcaagt cttgcgtcta
     4501 tggtggtata aaatgaaacc aactatagtt cgaaatcgac agatcttcca agccgcaaat
     4561 catacaaaga gtagacaact tggtgctact ttatcgaaag aactacgaga aaaatacggc
     4621 gcacgtaata cacgtgtgat caaaggtgat acagttcatg taatgcgtgg cgaattcaag
     4681 ggaatagatg gcaaagtgac acgtgtttca actgagaaaa acagtgttgc agttgaggga
     4741 ataaaacgag aaaaactcaa aggcggaaat atcgatattt tcattcacac gtcaaatgta
     4801 atgataactg acatgaatct tgatgacaag tggagacaaa acagactagc gggacaggca
     4861 acaaaaacac ctacagaaaa accaaaagag gaatcaaaag aaaaaccaaa accacaagaa
     4921 aaggtaaaaa caataaagga aaaaccaaaa gccgaaaaag aaactccaaa agaaaagact
     4981 gtaaaatctg aggaaaagaa aagaaaacaa acaaaagccg ttaaacctgc aaaaaaggag
     5041 aataagtaat ggtcagtata gcaggaagta agaaattaaa aaggcaaatg gctccagtct
     5101 tctggggaat aacaagaaag gattcacgat ttgttgttac agtaagaccg ggcggtcatt
     5161 caaagcacct ttcaattcca agcgctgtat ttctaagaga tacgctaaag ctagttacca
     5221 cattaagaga agcaaaatct gcaatctatg gaggtaaagt caaagttgat ggtgttaca
//
LOCUS       DATIDL010000022        13740 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrosopumilaceae archaeon isolate SMAG_U16342
            k141_2256685, whole genome shotgun sequence.
ACCESSION   DATIDL010000022 DATIDL010000000
VERSION     DATIDL010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935836
            Sequence Read Archive: SRR25158326
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosopumilaceae archaeon (soil metagenome)
  ORGANISM  Nitrosopumilaceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrosopumilales;
            Nitrosopumilaceae.
REFERENCE   1  (bases 1 to 13740)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13740)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/04/2024 05:09:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,520
            CDSs (total)                 :: 1,474
            Genes (coding)               :: 1,474
            CDSs (with protein)          :: 1,474
            Genes (RNA)                  :: 46
            rRNAs                        :: 1 (5S)
            complete rRNAs               :: 1 (5S)
            tRNAs                        :: 43
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13740
                     /organism="Nitrosopumilaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2256685"
                     /isolate="SMAG_U16342"
                     /isolation_source="soil"
                     /db_xref="taxon:2202732"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2286
                     /locus_tag="VLB45_00990"
     CDS             <1..2286
                     /locus_tag="VLB45_00990"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HSD04315.1"
                     /translation="NEIKKMIGDAKLDELTNSILFSKKHFVSYFRTISEPEPKMGNKI
                     AELGLDESIVQVLEEKGIERFYKFQEESLLEIMRGNNVIITAPTASGKTEAFVVPIMQ
                     KIRDERDSKGKILALFVYPTKSLSKDQLPKIKEIGDRLGIQVAIFDGDTKTSDRQKIF
                     ENLPQVIITNFDVIHYHMWHRSKFAALLNTVKFLVVDEAHVYSGIFGSNVHYVIKRLK
                     RICQKIQIIASSATLDNALPFCEQLFGVQMKLVSGSGKKGKTNFSMLFPSLRTQRSLM
                     VEIVKKLTSKKHKTLVFSNSHLNSELLAMQARKQKVDIKVHRAGLMANYRKSVETAFK
                     NDTLMAISATPTLELGIDVGSVDGVISSTIPVNRLVQRIGRAARKGQDGYAFLVLGND
                     PISQYYKNHPKDYFEDVEKIYIDPKNPFVEEFQVLAMACDKPIAKHELAEHSEIIDRH
                     VSAGNLVLVENRYVPNYVQIESLLNDYSIRGIGKSIDIFLNGKKVGERALPIALEELH
                     ESAVYFLAGTRYKVKELGYPEKMYAKIEYLPRDYPYYTKALTEEWPTIETIYEKRKAN
                     GIEVAFCKLHIQKRVYGYVNIELGYEITQGQRVLLEKPLDYDFVTKGIVFKSPRPIAE
                     IKKSENEEYVEASGYHATEHVVIEGSNMITGGVSQDLGGISLGTSGLIFVYDSAIGGN
                     GASRALYDRLEKAFERSLFILKECPCKSEAGCPRCTFSYRCGNNNEYLHKLSATEVLQ
                     RIKDGESTEVSEPVEGDKPLV"
     gene            2336..3163
                     /locus_tag="VLB45_00995"
     CDS             2336..3163
                     /locus_tag="VLB45_00995"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010191138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HSD04316.1"
                     /translation="MEKIAVVTGSSSGIGFETSLALAREGYYTFATMRDIKKGNTIKE
                     IAKKENLKLEVIELDVDKDQSVQDAINKIIKEKNRIDVVVNNAGYGLFGCLEDLSMDD
                     LKAQFETNFFGVVRVSQAIIPIMRNQKSGTIVNISSVAGRIGFPVSPAYISSKFALEG
                     LSESMRYELSPFGINIIIIEPGVIKTNFMPSTKKSMKPDSVYKEITDKVIMGITMMAE
                     MGTPPQEVAKTIIKALKSENPLPRYPVGNDAVMFLEAKKAKTDLEFEKYIKKELFSS"
     gene            3302..4870
                     /locus_tag="VLB45_01000"
     CDS             3302..4870
                     /locus_tag="VLB45_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase I"
                     /protein_id="HSD04317.1"
                     /translation="MKWKTLQHNGIAFPPPFETKGITIKIKGEKVLLNLDQEEMVYQW
                     AKKKDTPYVQDKVFCKNFLGDFVKVLPAKFKNISLSDIDFSEANKVVDKEKDAKLTLG
                     KDEKKKIATQRKEIKEKMKAKYGVAIIDGKQVDVANWMAEPPGLFIGRGDHPLRGKWK
                     PRITQKDVILNLGKEAKVPEGNWGKIIHESDFMWLASWEDYLTQKRKYVWLSDTSDLK
                     QERDQMKYDKAIKLSEHIKSVMDKVVKEMTARDEKTRRVATVCYLIFKTAMRVGDEKD
                     PDEADTVGATTLRVEHIKLKPDAIEFDFLGKDSVRWQKSLPIKEQDKTLYDNLKKFTE
                     KKKPDEQIFHDITSRHVNEFLGKIVKGLTAKVFRTYLATVVVTDYLKKVDKLDSKSEN
                     IKIYHAKLANLEAAITCNHKRTIPKNFDESLQKKKDALVKIKETKPKTEKQREKQKLR
                     EEKMKLTIELTEKTRDYNLGTSLRNYIDPRVFKAWTDQVGLEWEKLYTSALQKKFQWV
                     TKVDTNWKKIADTQ"
     gene            4910..5004
                     /locus_tag="VLB45_01005"
     tRNA            join(4910..4942,4962..5004)
                     /locus_tag="VLB45_01005"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4965..4967,aa:Met,seq:cat)
     gene            complement(5031..7217)
                     /locus_tag="VLB45_01010"
     CDS             complement(5031..7217)
                     /locus_tag="VLB45_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller domain-containing protein"
                     /protein_id="HSD04318.1"
                     /translation="MNNKIAMIVVAAIAVAGTAGLFYGLSPINSNKNTDTFLFNDTSD
                     IIPSSLQTSDELKKFSSPDEIKKFLIEAEARNTILHGGAIFLDRATAFEESGREGAPV
                     PADSKATGAVSLDEAASYSTTNVQVSNVDEPDFLKNDGKYAYVLSGDKLTIIEAYPAE
                     SANVVLKIGLDVQGQSLQNMFLNKDRLVIFYQGSGEQYSIQQYDFMPRPIYTPTTHAL
                     IMDVSDKENPKIVKDYEVNGNYYSARMIGDRAYFIVNNGVDYRYPIMPLLKESSRTII
                     TPDVYYFDNPEQYYNFNTITSFDIFGDEINSQTFMMGYTNTMYVSEDNIYLTYQKNFP
                     YAYYETHGKDRFFQIIVPLLPEDVQSQIRSIENSNLDFDDKWNKIADLLQETYNKMPE
                     NDRNALFDKIQKAIDEYEVKIQQDTRKTVIHKIAIDDGNLNYVSKGEVPGYLLNQFSM
                     DESGNRFRIATTSEFYTQQGTHLYNNVYVLDDNLNIVGSLEKVAQDESIYSTRFIGDR
                     LYMVTFERIDPFFVIDLSENTPRILGALKIPGYSNYLHPYDENHIIGIGKETKENKYG
                     GIETLGVKVALFDVSDVQNPKEVDVYTVGGQGTDSEILWDHKALLFSKEKNILSIPIW
                     EQMPYDEPVPLDGVSRPYIEPKAWRGFYVFGIDVNDGFSLKGKVEHISDNNYYWYGYG
                     SRSFYIGDVLYTVSPNLMKMNDLNDINREINQIKLENTGKIIPYLE"
     gene            7404..8594
                     /locus_tag="VLB45_01015"
     CDS             7404..8594
                     /locus_tag="VLB45_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04319.1"
                     /translation="MEEFDEFALALLDQLSVEISEEREITASSSKATQALASKVKFQD
                     IKLLSDKIFPLLADKVFEFTGIPVSPQTKVELLDLVELKRLKGRKVFPTKESEAYVDE
                     LFTAIANEKADKIAELMKKDIMRFLVYSTYAKSYISKISTTYGDYLDSKIFLNKFVLS
                     SYPQIILQKQGEPYEAKFEYVNSGYIGALKMTILEEQIHSIQTNLQEVNKQAVIEVNV
                     LNEELAKIILGLDEITVNKLSEYLQLPQVPDEFPIAKKANLFFMLNPDNFIVNVLGPD
                     VMTFTKVTVDPKISEMLPQLLDIYQRWLGPIQTHHAAFTTMEGMAEFAVQNILKDDED
                     FQNYLVTFANTDITTYQVRKSMGKDFTEFVFAKLGKNTYHTLIENPPTTRELKDPEKY
                     LQRL"
     gene            8591..9415
                     /locus_tag="VLB45_01020"
     CDS             8591..9415
                     /locus_tag="VLB45_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HSD04320.1"
                     /translation="MSQDFDPFVAELITYVTNPRYTLVEKCLKMAQIFEYSDLSIVEY
                     VSKLNNMGKVLRDSISDVKNPTYLISMLNEYMFDTLGFTGNHDDYFNPKNNFLNVVMD
                     TKVGIPITLSIIYVEIAKHIGLDLRPVGFPSHFLVKQSEEIILDPYGRGRLLTIDDLN
                     EILERSYGGEVEFMPQFLDEIEPEKIIIRIARNLKNSYTQSFNYDMAMHCINLILGIE
                     PTAPEEIRDRGILEARLLHYDLALESLNKYLELMPEAEDVDYVLDLIRGIRERANQ"
     gene            complement(9405..9578)
                     /locus_tag="VLB45_01025"
     CDS             complement(9405..9578)
                     /locus_tag="VLB45_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04321.1"
                     /translation="MSGENEESIYERVARLEDEVAALKNEIDVIKGAIRNKIARHEIG
                     KIRSGKDVTSIID"
     gene            complement(9651..9812)
                     /locus_tag="VLB45_01030"
     CDS             complement(9651..9812)
                     /locus_tag="VLB45_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04322.1"
                     /translation="MVRRKTVKPKASSPRNVTTGLCPKCGTIGKILIIGVTGMEKGKC
                     QACNAEFVL"
     gene            complement(9868..9951)
                     /locus_tag="VLB45_01035"
     tRNA            complement(9868..9951)
                     /locus_tag="VLB45_01035"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9915..9917),aa:Ser,seq:gct)
     gene            complement(9990..10736)
                     /locus_tag="VLB45_01040"
     CDS             complement(9990..10736)
                     /locus_tag="VLB45_01040"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007551218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HSD04323.1"
                     /translation="MKIVQLSDIHVGSQFREEAFDKVVEEVNDLGPSAVLVTGDLTNE
                     GLLKEYEKCKQKFAKFEVDKIIAISGNHDYRNTGYLLFKKFFPFETVNMLGDDIVLVT
                     LGTARPDRDEGEVGYRQNLWVERTMKKYPKKIKIVAMHHHLIGIPDSGTDRVTVIDAG
                     DVLRTALSSQVDLVLCGHKHRPWIWDFGNLSIANAGTASSERMRGFFENTYNIITIEK
                     HKIKVDLKIVGGKRFPLKDLVTNYTRFGDE"
     gene            10873..11901
                     /locus_tag="VLB45_01045"
     CDS             10873..11901
                     /locus_tag="VLB45_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HSD04324.1"
                     /translation="MVKQAAIIKGDGTGPELVDAMLRVLKSCNTQSELILCEAGSEQW
                     EKSGGATYIPDSTMKLLEESDACYKGPTTTIPKPNAPRSVAVTIRQKFELYSNIRPIK
                     TYDRLSPNKKLDFVCFREATEGLYAGIEFRISEDSAIAIRKTTRKGCQRIVLAAFSWA
                     KKYNLKKIFAITKRNILKETDGIFWSEVEKAARDNPGIEVEEYYIDNMTQQLVKNPER
                     FNSSVLLSTNLFMDIISECASGNIGSIGNVYSANIGDSYAMFEPAHGSAPKYKGMDKV
                     NPTAAILSGAWMAEYLGEPQIKDAIFSATEQVINEGKYVTYDIGGNASTSKMSEVIAE
                     IAKQKLRK"
     gene            11995..12399
                     /locus_tag="VLB45_01050"
     CDS             11995..12399
                     /locus_tag="VLB45_01050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04325.1"
                     /translation="MLIGLIIISPTGSAIQAEAGNSTQSESNTISIPSWIKKNAGWWA
                     GGQIEDKEFVQGIQYLIQKGIMKIPETKASESKSSQQIPAWIKNNAGWWADGKISDSD
                     FVLGIQYLISKGIMRVGSLPTTPKDTCTGYYC"
     gene            complement(12428..12961)
                     /locus_tag="VLB45_01055"
     CDS             complement(12428..12961)
                     /locus_tag="VLB45_01055"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HemK2/MTQ2 family protein methyltransferase"
                     /protein_id="HSD04326.1"
                     /translation="MQNKLSKSEYYSPAEDTLFFANQIQNEKGQFALDIGTGSGYLAK
                     VLLPNFDLVVATDIIFDTAKKAHSIIENCVCCNAADALHAEFDLIVCNMPYLPSDKIT
                     DVTVDGSHEGVTVPLKIIKSVKNVLKKGGRMLFLTSSLANYKELLKQAESLGFKVKII
                     ATTKMFFEELFLVEATK"
     gene            complement(12936..13646)
                     /gene="rsmA"
                     /locus_tag="VLB45_01060"
     CDS             complement(12936..13646)
                     /gene="rsmA"
                     /locus_tag="VLB45_01060"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010189880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HSD04327.1"
                     /translation="MNKRHFLGQHFLISESIAKAIVDHADITKKDEVLEIGTGKGILV
                     PYLCKLANQVTSIETDRELYAQALEKFSNLKNLSLKHGDGFKLDSDFTIFVSNLPYSE
                     SRNAIEWLAQKKFSHAVIMVQKEFAEKLLAKPGDKNYRAITILANHCFKIEPLMDVKK
                     ANFSPSPKVDSVVLRLDCKHKIPKNLVSTVNKLFSYRRKTIHNVAKQFGVSIDSNQRL
                     EDLTYGEIIKLAKQIIKI"
BASE COUNT         4598 a         2440 c         2644 g         4058 t
ORIGIN      
        1 aatgagataa aaaagatgat aggagatgcc aaacttgatg aactgacaaa ctctattttg
       61 ttttcaaaga aacattttgt ttcttatttt agaacaatat cagaaccaga acccaagatg
      121 ggcaacaaaa tagcagaatt aggattagac gagagcattg ttcaagtttt agaagagaaa
      181 ggcattgaac ggttttacaa gttccaagaa gaatcgttat tggaaataat gcgtggaaac
      241 aacgtaatca tcactgcgcc cacagcatca ggcaagacgg aagcatttgt tgtacctatc
      301 atgcagaaga ttcgtgatga gagagactct aaaggtaaga ttctggcact ttttgtttat
      361 cctacaaaat cgctttcaaa ggatcaactg ccaaagataa aagagattgg cgacaggttg
      421 ggaatacagg tggccatctt tgatggagat acaaaaacat cagacagaca aaaaatattt
      481 gaaaatctgc cccaggtcat aatcacaaat tttgatgtca tacattatca tatgtggcac
      541 cgaagtaagt tcgcagcgct gttaaacacg gtaaaatttc ttgtggtgga tgaggcccat
      601 gtatattcag gaatatttgg atcaaatgtt cattatgtaa ttaaaagact gaaaagaata
      661 tgccaaaaga tacagatcat cgcgtcatct gcaacgctgg ataatgcatt accattttgt
      721 gaacagctct ttggagtgca aatgaaactg gtttctgggt ctggaaaaaa aggaaagaca
      781 aattttagta tgctctttcc atcacttaga acacaacgtt ccttgatggt ggaaattgta
      841 aaaaaattaa catcaaaaaa acacaagact ctagtcttta gtaattcgca tctaaattcc
      901 gaacttttag ctatgcaggc aagaaaacaa aaagtggaca tcaaggttca cagagcaggt
      961 ctaatggcaa attacagaaa atcagttgaa accgcattca aaaatgatac ccttatggca
     1021 atttcggcta cacccacact tgagcttgga atagatgtag gaagcgtaga cggtgtaatc
     1081 tcttctacca ttcctgtaaa cagactggtg cagagaatag gcagggctgc aagaaagggt
     1141 caggatggat acgcattttt ggtgcttgga aacgacccaa tctcacaata ttacaagaat
     1201 catccaaaag actactttga agatgtggaa aagatctaca ttgatcccaa gaatccattt
     1261 gtggaagaat tccaagtact tgcaatggca tgtgacaaac caattgcaaa gcatgagtta
     1321 gcagaacatt cagaaatcat cgacagacac gtatctgctg gaaatcttgt tttagtagaa
     1381 aatagatacg ttccaaatta cgtacagatt gaatccttgt taaatgatta tagcatcagg
     1441 gggattggca aatcaattga catatttttg aatggaaaaa aagttggtga acgtgctctt
     1501 ccaattgcgc ttgaagagct gcacgaatcg gcagtttatt ttcttgcagg aacaagatat
     1561 aaagtaaaag agcttggcta tccagaaaaa atgtatgcaa agatagaata tcttccaaga
     1621 gactatccat actataccaa agcgttaaca gaagaatggc caaccattga aactatttat
     1681 gaaaaaagaa aagcaaacgg aatcgaggtt gcattttgta aattacacat acaaaaaaga
     1741 gtatacggtt atgtgaacat agaactcggt tatgaaatca cccaaggaca aagagtgctt
     1801 ttagaaaagc cattggatta tgattttgtt acaaagggaa ttgtgtttaa atctccaaga
     1861 ccaatagcag aaataaaaaa atcagaaaat gaggaatatg tggaagcaag tggatatcat
     1921 gctacagaac atgtggtaat agaaggcagc aacatgataa cggggggggt ctctcaagac
     1981 ttgggaggca tatccctagg aacgtcaggt ctcatctttg tctacgattc ggccattggt
     2041 ggaaatggtg caagtagagc cctttatgac agactggaaa aggcgtttga gaggagttta
     2101 ttcatactaa aagaatgtcc gtgtaaaagc gaggcaggat gtccaaggtg cactttttcg
     2161 tacagatgcg gaaacaacaa cgaatacctt cacaagcttt cagcaacaga agttttgcaa
     2221 agaatcaaag acggagaaag caccgaggtt tctgaacctg ttgaaggaga caaacctctg
     2281 gtctgatcat cattacaata atacaaatta ttaatagcgt tttcagacag catttatgga
     2341 aaaaattgca gttgttacag gaagctcaag cggaattggt tttgagactt cgcttgcttt
     2401 agcaagagaa gggtactata cttttgcaac catgcgcgat ataaaaaagg gaaatacaat
     2461 aaaagaaatt gcaaaaaaag aaaatctgaa acttgaagtc atagagcttg atgtggacaa
     2521 agatcaaagt gtacaagatg caatcaataa aataattaaa gaaaaaaata gaatagatgt
     2581 tgttgtgaat aatgctggat atggtctctt tggttgtctg gaagatcttt ccatggatga
     2641 ccttaaggca caatttgaaa caaacttttt tggcgtggtg cgagtttccc aagcaataat
     2701 tcccataatg cgtaaccaaa aatctggaac aatagtaaac ataagctcag tagcaggcag
     2761 aattggattt cctgtttcgc cagcttacat tagctcaaag tttgctcttg aaggattgag
     2821 tgaatccatg agatacgagc tctcaccatt tggcattaac atcataataa tagaaccagg
     2881 tgtgataaag acgaatttca tgccatctac aaaaaaatca atgaaaccag actctgtcta
     2941 caaggaaatt acagacaagg tgatcatggg aattaccatg atggcagaaa tgggcacacc
     3001 tccacaagag gtagcaaaaa ccataatcaa ggcattaaag tctgaaaatc cattacctag
     3061 atatccagta ggaaatgatg cggtaatgtt tttggaggca aaaaaggcaa agacagatct
     3121 agagtttgaa aaatatatca aaaaagagct ttttagttct tagaatatct ttttagagca
     3181 atatcaaaac gtaacacatc agtacatatg attgctttac aggttacgtt gatatactac
     3241 gcccattcaa tttttatcaa agtcaaccga tttaggcact aatttggtga tgatttagaa
     3301 catgaaatgg aaaactctac aacataatgg catagccttc cccccgcctt ttgaaacaaa
     3361 ggggataacg atcaagatca aaggtgagaa ggtgttgctg aatttagatc aagaagagat
     3421 ggtataccaa tgggcaaaga aaaaagacac tccatatgtt caagacaagg tcttttgcaa
     3481 aaatttccta ggcgattttg taaaggtatt gcctgcaaaa tttaaaaaca tttcactctc
     3541 tgatattgat ttttctgaag caaacaaagt agtagacaaa gaaaaagatg ccaagcttac
     3601 cttgggtaag gatgaaaaaa agaagattgc tacacaacga aaagagatca aagaaaaaat
     3661 gaaggcaaaa tacggggttg caataattga tggaaaacaa gtcgatgtag caaactggat
     3721 ggctgaacca ccaggacttt tcattggaag aggagaccac cccttgagag gaaagtggaa
     3781 acctcgaatt actcaaaagg atgtcatact aaacctcgga aaagaagcca aagtgccaga
     3841 aggcaactgg ggcaagataa tacacgagtc tgactttatg tggcttgcaa gttgggaaga
     3901 ctatcttact caaaaacgca agtatgtctg gctttcggat acatcagatt tgaaacaaga
     3961 acgcgaccaa atgaaatacg acaaggcaat aaaactttct gaacacatca aaagtgtgat
     4021 ggacaaagtt gtaaaggaaa tgacagcacg cgatgaaaaa acaagaagag ttgctactgt
     4081 gtgttatttg attttcaaaa cagctatgag ggtaggagac gaaaaagatc ctgatgaagc
     4141 agacactgtt ggtgcaacca cactaagagt tgaacacatc aagctaaaac cagatgcaat
     4201 agaatttgat ttcttaggca aagatagtgt aagatggcaa aaatctcttc ccataaaaga
     4261 acaagacaaa acactgtatg acaacctgaa gaaatttacc gaaaagaaaa aaccagacga
     4321 acagattttt catgacatta catcaaggca tgttaatgaa tttcttggaa aaatagtgaa
     4381 aggacttact gccaaagtct ttagaacgta tttggcaaca gttgttgtaa cagattatct
     4441 caaaaaagta gacaaattag attcaaaatc agaaaacatc aaaatttacc atgccaaact
     4501 agcgaatcta gaggctgcaa ttacctgtaa tcataaaagg actatcccaa agaactttga
     4561 tgaatcctta cagaaaaaga aagatgctct agtaaagata aaggaaacaa agccaaagac
     4621 ggagaaacaa cgcgagaagc agaaactgag agaggagaaa atgaagctca caatagagtt
     4681 aactgaaaaa acaagagatt acaatttggg aacatcactt aggaactaca tagatccgcg
     4741 tgttttcaaa gcatggactg accaagtagg attggagtgg gaaaaacttt acacttctgc
     4801 acttcagaag aagtttcagt gggttactaa ggtagatacc aactggaaaa aaattgcaga
     4861 cactcagtaa gctacattta attgcatcaa aaaattaggc gtaatctgtg ccggggtagc
     4921 tcagcctggc ctagagtgcc agtctccata caaagggtaa tactcataat ctggaggtcg
     4981 tgggttcgaa acccaccccc ggtatacacc ttttttctga gctgctttga ttattcaaga
     5041 taaggaatta ttttaccagt attttcaagc ttgatttggt ttatttcacg attgatatcg
     5101 tttagatcat tcatcttcat caagtttggt gaaactgtat acaaaacgtc accaatgtaa
     5161 aatgatctgc ttccataacc ataccagtag taattgttgt cactgatgtg ttctacttta
     5221 cctttcaagc tgaatccatc attgacatca attccaaaca cgtagaatcc tcgccaagct
     5281 tttggttcaa tgtatggtcg tgaaactcca tcaagaggaa caggctcatc gtatggcatt
     5341 tgttcccaga ttggaattga tagaatgttt ttttctttac tgaacaatag agccttgtga
     5401 tcccataaaa tttcagaatc agttccttga ccaccaaccg tatacacatc tacttctttt
     5461 ggattttgaa catctgagac atcaaatagt gctactttga caccaagtgt ttcaattccg
     5521 ccgtacttgt tttccttggt ttccttgcct attccgatta tgtggttttc atcatatgga
     5581 tgaagatagt ttgaatatcc tggtatcttt aatgccccca agattcttgg tgtgttctct
     5641 gaaagatcaa ttacaaagaa cgggtctatt ctttcaaatg tcaccatgta caatctgtca
     5701 ccaatgaatc tagtagagta aattgactcg tcttgtgcta ctttttcaag acttcctaca
     5761 atgtttagat tatcgtcaag gacatagaca ttgttataca aatgtgtgcc ttgctgtgta
     5821 taaaattccg atgtggttgc aattctaaat ctattgcctg actcgtccat tgaaaactgg
     5881 ttgagcaagt atccaggtac ttcgcctttt gaaacatagt ttaggttgcc gtcatctatt
     5941 gcaattttat gaattacagt ttttcgagta tcttgttgta ttttgacttc atactcatca
     6001 attgcttttt gaattttatc aaatagtgca tttctatcat tttcaggcat tttgttgtaa
     6061 gtctcttgta gcaagtctgc tatcttgttc cacttatcgt caaaatccaa gttactattt
     6121 tctatggatc gtatttgtga ctgtacgtct tctggcaaaa gcggcactat gatttgaaag
     6181 aatctgtctt taccatgagt ctcataatat gcatatggaa agtttttttg ataggtgaga
     6241 tagatgttgt cttcagaaac atacatcgta ttggtgtagc ccatcatgaa agtttgtgaa
     6301 tttatttcat caccaaaaat gtcaaatgaa gttatcgtat tgaaattata atactgctct
     6361 ggattatcaa aataatatac gtctggggta atgatggttc gtgatgattc ctttaggaga
     6421 ggcattattg gatatctgta atcaactccg ttgtttacga taaagtacgc acgatcccca
     6481 atcattcttg cactatagta attgccattt acttcatagt ctttgacaat ctttggattt
     6541 tctttgtcag agacatccat tatcaatgca tgtgttgtag gtgtgtagat tggtcttggc
     6601 atgaaatcat attgttgtat ggagtattgc tctcctgatc cttggtagaa aatcactagt
     6661 ctatctttat tcaaaaacat gttctgaaga ctttggcctt gcacatccaa accaatcttt
     6721 agtacaacgt tagcactttc tgctggatat gcttcaatta ttgtgagttt gtctccagat
     6781 agaacgtaag catattttcc atcattttta agaaaatcag gttcgtctac gttgctaacc
     6841 tgaacgtttg tagtagaata tgacgcagct tcatctaaag atacagcccc ggtagcttta
     6901 gaatcagcag gtactggtgc tccttctctg cctgattctt caaatgcagt tgctctatca
     6961 agaaaaattg cgcctccatg cagaattgta tttcttgctt cagcttcaat caaaaatttc
     7021 tttatctcat ctggtgaaga gaattttttt aattcatcac ttgtttgaag tgaagatgga
     7081 attatgtctg atgtatcatt gaaaagaaaa gtgtcagtat ttttattaga attaattgga
     7141 gacagtccat aaaataatcc tgcagtgcca gctactgcaa tagcagcaac tacaatcatt
     7201 gcaattttat tattcataga ttagatcctc cgcgatattc aattaaggct tgcgtagcta
     7261 tcattccagt aattatcaaa atagtagcta aagtataaga ggtttgatta aattgaaatt
     7321 gaagttatca ttttgtcaaa aaatatctga gcatcaatca atacgttaac gttaaattgg
     7381 ttcagatatt cacaatcaga ataatggaag agtttgatga atttgctttg gcattacttg
     7441 atcagttgtc agttgagata agtgaagaaa gagagataac agcatcgtca tcaaaagcaa
     7501 cgcaagctct agcatccaaa gtaaaatttc aagacatcaa attattatca gacaaaattt
     7561 ttcctttgtt agcagacaaa gtgttcgagt tcacaggcat ccctgtttca cctcaaacaa
     7621 aagtcgaact tttggatttg gttgaactca aaagactaaa ggggagaaaa gttttcccca
     7681 caaaagaatc cgaagcatat gttgatgagc ttttcacagc aatagccaat gagaaagcag
     7741 acaagatagc agaattgatg aaaaaagaca tcatgcggtt tctggtctat tccacgtatg
     7801 cgaaatcata catctcaaag atatctacca cgtatggaga ctatcttgat tcaaagatat
     7861 ttttgaataa atttgtcctt tctagttatc ctcagatcat tttacaaaaa caaggcgagc
     7921 catatgaagc caaattcgaa tatgtaaatt ctggctacat tggagccttg aagatgacaa
     7981 tacttgaaga gcagatccat tcaatccaga caaaccttca ggaggtaaac aagcaggcag
     8041 tcattgaggt aaatgtacta aatgaagagc ttgcaaaaat aatcctcgga ttagacgaga
     8101 ttacagtaaa caaactttca gaatatctgc agttacccca agtccctgac gaatttccca
     8161 tagccaaaaa agctaatctc tttttcatgc taaaccctga caattttatt gtaaatgtgc
     8221 ttggtcctga tgtgatgact tttaccaaag taacagtgga tccgaaaatt tctgaaatgc
     8281 taccacagtt attagatatt tatcagagat ggcttggacc aatccaaact catcacgctg
     8341 catttactac gatggaaggc atggcagagt ttgcagttca aaatattcta aaagatgatg
     8401 aggactttca gaattatctt gttacgtttg ctaataccga cattacaaca taccaggtac
     8461 gcaaaagcat gggaaaggac tttacagaat ttgtgtttgc aaagcttggc aaaaacacgt
     8521 accacacttt gattgaaaat cctccaacaa caagagagtt aaaggatcca gagaaatacc
     8581 ttcagagatt atgagtcagg attttgatcc ttttgttgcg gaattaatca catacgttac
     8641 aaatcctaga tatacgctgg tggaaaaatg cctcaagatg gcacagattt ttgaatactc
     8701 agatttgagc atagtagaat atgtctcaaa attaaacaac atgggaaaag tattgcggga
     8761 ttctatttca gatgttaaaa atccaaccta tcttatttcc atgttaaatg aatacatgtt
     8821 tgatacactt ggatttacag gaaaccatga tgattacttt aatccaaaaa ataatttttt
     8881 aaatgttgta atggatacaa aagttggcat tcccataacc ttgtcaatta tctatgtgga
     8941 aattgcaaaa cacataggat tagatttgcg tcctgttggc tttccaagtc actttcttgt
     9001 caagcaatca gaagagatca tcttggatcc atatgggcgt ggacggctac ttacgataga
     9061 cgatctaaac gagattttgg agagaagcta tggaggggaa gtagaattca tgccacagtt
     9121 tttagatgaa atcgaaccag aaaaaatcat aatacgaatc gcaagaaatt tgaaaaattc
     9181 ttacacgcaa tcgttcaact acgacatggc aatgcattgc ataaatctga ttcttggcat
     9241 cgaaccaact gcgccagagg aaattagaga cagagggatt ctggaagcaa ggctcttgca
     9301 ttatgattta gccctagaat cattaaacaa atacctagaa cttatgccag aagctgaaga
     9361 tgtagattat gttcttgatt tgataagagg aatcagggaa agagctaatc aataatcgac
     9421 gtaacgtctt ttccgcttct tatcttgcct atttcatggc gtgcaatctt gtttcgtata
     9481 gcgcctttta ttacgtcaat ttcgtttttt agagcggcta cttcatcttc aagtcttgct
     9541 accctctcat agatggattc ttcgttttct ccgctcatac cgatctaaag gataaaaatg
     9601 accttaaaaa tttatgggtc atttttttga tagtttggtt agtttttatt ctatagtaca
     9661 aattcagcgt tacaagcttg gcacttgccc ttttccatgc cagtgacgcc aataatcaaa
     9721 attttgccga tagtaccgca ttttggacac aggccggtag ttacatttct tggagaactt
     9781 gcctttggtt ttacagtctt tctgcgtacc attatttcag aaaccagaaa tcggattatt
     9841 aagtctaagc gtttggatcg caaaaagcgc ggggagtggg attcgaaccc acgggtcctt
     9901 gcggacatgg gattagcaat cccacgccct accaggctag gcgacccccg cacaaggtgc
     9961 tgaaaattaa aactgttaat taatctagct tactcgtcgc caaaacgagt atagtttgtg
    10021 acaagatcct ttaatggaaa acgcttgcca ccaacaattt tcagatctac tttgatcttg
    10081 tgtttttcta tggttatgat gttgtaagtg ttttcaaaaa aaccacgcat tctttctgaa
    10141 gatgcagtac cagcgtttgc tattgaaaga tttccaaaat cccaaatcca cggtctgtgt
    10201 ttgtgaccac atagtacaag atcaacttgg cttgaaagtg cagtccgcag tacatctcct
    10261 gcatctatta ctgtaactct gtctgtgcca ctgtctggaa ttccaatcaa atggtggtgc
    10321 attgcaacta tcttgatctt ttttggatat tttttcatcg tgcgctctac ccacaagttc
    10381 tgcctgtatc ccacttctcc ctcatctcta tcaggtctgg cagtgcccag agttacaagg
    10441 acaatgtcgt ctccaagcat attgactgtc tcaaagggaa agaatttctt gaataacaga
    10501 tagccagtgt tcctataatc atgatttcca ctgattgcaa ttattttatc tacttcaaat
    10561 tttgcaaatt tttgtttgca tttttcatat tcttttagca gaccttcatt tgtcaggtca
    10621 ccagtaacta agactgctga aggaccaaga tcgtttacct cctctactac tttatcaaaa
    10681 gcttcttcac gaaattgcga gccaacatgt atgtcggaaa gttgtactat tttcatttaa
    10741 caatattgaa tacagaatca ttgtttaagc attaggtttt tttcaacaat tgtgattttg
    10801 tttataattt gcgtaaaaga tcaaaacttg ctagttagag ttaaatatgc tagatctaca
    10861 aacttgtctg ttttggtaaa gcaagctgcg atcataaaag gagatggtac tggacctgag
    10921 cttgttgatg cgatgctacg tgttttgaag tcatgtaaca cacaatctga gcttattttg
    10981 tgtgaagctg gttcagaaca gtgggaaaaa agtggtggtg ctacatacat tccagattcc
    11041 acgatgaaac ttcttgaaga aagtgatgcg tgctacaaag gaccaactac tacaatacca
    11101 aaaccaaatg cacctagaag tgttgcagtc accattcgcc agaaatttga actttatagt
    11161 aacataagac cgatcaagac atatgataga ttatcaccaa acaaaaaact tgactttgta
    11221 tgctttaggg aagctacaga gggactttac gcaggaattg aatttagaat atctgaggat
    11281 tcagccatag caataagaaa aactacacgt aaaggttgcc agagaatagt actggcagct
    11341 ttttcatggg caaagaaata caatctaaaa aagatctttg cgattacaaa gcgaaatata
    11401 ctaaaagaga cggatggcat attttggtcc gaagttgaaa aggcagcaag agacaatcct
    11461 ggaatcgaag ttgaggaata ctatattgac aatatgacac agcaactagt taagaatcca
    11521 gaaagattca acagttcagt attgttaagc acaaatctgt ttatggatat aatatcagaa
    11581 tgcgcatctg gaaacattgg ttctatagga aacgtgtact ctgcaaacat aggagattcg
    11641 tatgcaatgt tcgagccagc tcatgggagt gcaccaaaat acaaaggcat ggacaaggta
    11701 aatcccaccg ctgcaatatt gtcaggtgcg tggatggcgg aatatcttgg cgaacctcaa
    11761 ataaaagatg caattttttc agcaacagaa caagtaatca atgagggaaa atatgtcaca
    11821 tatgatattg gcggtaatgc aagtacgtca aaaatgtctg aagtaattgc agaaattgca
    11881 aaacaaaaac tcagaaaata atatacttaa tcttctctat ggaagataca atttatgagt
    11941 aatatctaaa agtgatactg acatattgta aatacgaaaa ctattttcat tttggtgtta
    12001 attggtctaa taattatatc acctactggt agcgctatcc aagcagaagc aggcaactcc
    12061 acacaatcag aatcaaacac aatatcgatt ccaagctgga taaagaaaaa tgcaggatgg
    12121 tgggctggcg gtcagataga agacaaggag tttgtacaag gcatccagta tctcatacaa
    12181 aagggaataa tgaaaatacc tgaaaccaaa gcttctgaat caaaatcatc acagcaaatt
    12241 ccagcgtgga ttaaaaacaa tgctggatgg tgggcagatg gtaagatctc cgatagcgac
    12301 tttgtgttag gaatacagta tctcattagt aaaggaataa tgagagttgg ttctcttcct
    12361 accacaccca aggatacatg cactggatat tattgttaaa accactggaa gatgaaaaac
    12421 tgtttaatta ttttgtagct tcaacaagaa ataattcttc aaaaaacatt tttgttgttg
    12481 cgataatttt gaccttaaat cctaatgatt ctgcttgttt taacaactct ttgtaattag
    12541 ctagagatga cgttagaaaa agcatccttc ctcctttttt gagtacgttt tttaccgatt
    12601 ttattatttt gagaggaact gttactcctt catgcgaacc atctactgtt acatctgtga
    12661 ttttatcaga tggtagatat ggcatgttgc atactatcaa atcaaattct gcgtgtaatg
    12721 catctgcagc attacaacag acacaatttt caattataga atgtgctttt tttgcagtat
    12781 caaatataat gtcagtggca accacaagat caaagttggg caacaatact tttgccaaat
    12841 aaccagagcc agttccgata tcaagtgcaa attgcccctt ttcattttgg atttggtttg
    12901 caaaaaatag cgtatcttct gcaggagaat agtactcaga ttttgataat ttgttttgca
    12961 agttttatta tctcaccata tgtcaggtct tctagtcgtt ggtttgaatc tatcgataca
    13021 ccaaattgtt ttgcgacatt atgaatcgtt tttcttcgat atgaaaacag tttgtttact
    13081 gtacttacca gattctttgg gatcttgtgt ttacaatcca gtcttaacac gacagaatca
    13141 acttttgggg aaggagaaaa gtttgctttt ttcacatcca tgagaggttc tattttgaaa
    13201 caatggtttg ccagtatggt tattgctcta tagtttttgt ctccaggctt tgcaagtaat
    13261 ttttcagcaa attctttttg aaccataatt acggcatgag aaaatttctt ttgagcaagc
    13321 cattctattg cattacgact ctctgaatat ggcaaattag agacaaaaat ggtgaaatcc
    13381 gaatccagtt taaagccgtc accatgtttt aggctcagat tttttagatt tgagaatttt
    13441 tccaaggcct gtgcatatag ttccctgtct gtttctattg atgttacttg gtttgcaagt
    13501 ttgcaaagat atgggaccag tattcctttt cctgtgccaa tctcaagcac ctcatccttt
    13561 tttgttatat cagcatgatc aactatagct tttgcaatag attctgaaat cagaaagtgt
    13621 tgtcctagga aatgtcgttt attcatcgtc taacaaagag gctcattctt gattcccctg
    13681 ttatttcctc aaggatcctc tttgcgattt gttttactgg ttcttttagt ccaactctat
//
LOCUS       DATIDL010000023        13286 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrosopumilaceae archaeon isolate SMAG_U16342
            k141_36582, whole genome shotgun sequence.
ACCESSION   DATIDL010000023 DATIDL010000000
VERSION     DATIDL010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935836
            Sequence Read Archive: SRR25158326
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosopumilaceae archaeon (soil metagenome)
  ORGANISM  Nitrosopumilaceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrosopumilales;
            Nitrosopumilaceae.
REFERENCE   1  (bases 1 to 13286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/04/2024 05:09:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,520
            CDSs (total)                 :: 1,474
            Genes (coding)               :: 1,474
            CDSs (with protein)          :: 1,474
            Genes (RNA)                  :: 46
            rRNAs                        :: 1 (5S)
            complete rRNAs               :: 1 (5S)
            tRNAs                        :: 43
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13286
                     /organism="Nitrosopumilaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36582"
                     /isolate="SMAG_U16342"
                     /isolation_source="soil"
                     /db_xref="taxon:2202732"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1425
                     /locus_tag="VLB45_01065"
     CDS             <1..1425
                     /locus_tag="VLB45_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthesis decarboxylase"
                     /protein_id="HSD04328.1"
                     /translation="NEFVEKLENAGELKRVKTQVDADLEIAEILRRTMYSNGPALFFE
                     NVKNHTMPVLANAFGSLKRLEIGLETSDFTQIGQRIVDMTKMDIPSGLFDKIRKLPEL
                     SKMTDIAPKLQKSGPVTEIFEDAPSFEKIPILKTWHKDAGRFITFGLVATRHPETGIR
                     NLGVYRMQILDKTHALMHWQKHKRGAHHYDIKKDKGDKIEVAIVIGGDPATVFSAVAP
                     VPEGLDKYLFAGIVRKTGIKTVKCKTVDLEVPANAEMVLEGYVDPSDIRMEGPFGDHT
                     GYYTPPEPYPTFTLTGIMRREKPIYLTTIVGKPIVEDAFIGKVIERSFLPLIKMFQPE
                     VMDFSMPAAGWFQGMAIISIKKRYPGQAKKVMMGLWGLGQLALTKIFVVVDDDINVHD
                     MNDVIWAMTTRADAARDVMIVENTPTDTLDPASPLVNLGSKLGIDATQKTREEGYQRE
                     IQEVVKVDEDTRNVVDAKWSSYGI"
     gene            complement(1422..2540)
                     /gene="mqnC"
                     /locus_tag="VLB45_01070"
     CDS             complement(1422..2540)
                     /gene="mqnC"
                     /locus_tag="VLB45_01070"
                     /EC_number="1.21.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic dehypoxanthinyl futalosine synthase"
                     /protein_id="HSD04329.1"
                     /translation="MSQITKELYASEIGDILENSLDGKRPELEDCIRLLESNDVHLMG
                     LVAGYMTRKLYGKKASFVNNMILNYTNVCITDCKFCAFYRPPDHEESYTVSIEQIESR
                     VKAAWEMFGITQVLIQGGHNPKLGIEYYEDAFRLIRKKFPKVGVHGLSASEIDMIARV
                     EKSSTQEVLARLKEAGLQSVPGAGAEILVDSVKDVISPKKISSEQWLKIMEEAHSMGL
                     PASATMMYGHVETPHDIAEHFVKIAKLQEKTGGFMAFIPWSFEPNNTLMQEEKTVLYP
                     AGGYQLLKMIAISRIMYDGLIPHIQSSWLTNGVGMAQAALQYGADDFGGTLLGEEVVS
                     CTGARSTELTANKIIHAVKQIGFEVEERDNFYNLVQMH"
     gene            2642..3430
                     /locus_tag="VLB45_01075"
     CDS             2642..3430
                     /locus_tag="VLB45_01075"
                     /EC_number="2.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007550026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-3,7-dideoxy-D-threo-hept-6-ulosonate
                     synthase"
                     /protein_id="HSD04330.1"
                     /translation="MVTGLQIRLNRILRSGKMLCIPMDHGISSGPIKGLEDPHNVIYT
                     CQHYGLTSVIVNKGILKTLPKVPQVGVLVHFSASTSLSKTPNRKMLSGSVEEAVRLGA
                     DGVSLHINIGGKEEPEMLEQLGKISDDCHKWDMPLLAMMYPRGENIKDPHDPEIVAHV
                     ARIGAEMGADIVKTLYTGDFDSFASIVKSTPVPIVIAGGPKAKTDKDVLEMTEEAMRA
                     GAKGVTYGRNIFEHKHPDRITEALAGIIFRKETAKEAAKRLDKK"
     gene            3417..4475
                     /locus_tag="VLB45_01080"
     CDS             3417..4475
                     /locus_tag="VLB45_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007401287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase II"
                     /protein_id="HSD04331.1"
                     /translation="MTKSRELIISPKVSRGQLGKFLVEIEREGIKTVNVDPQSLKGIK
                     TKLTTIHSSPSANYVILDKKTTKPKGKKIGIKFKVLSNVDIEKILADAKTGLDFVIVE
                     VKDWKIIPLENIIAKLHKLHTKIFATANNPEEVRKMFSILEIGVDGVIFSTNSINEVR
                     EALVYLGTKSFELKPARVLEIKEVGNGERVCVDTASMLGRGEGMLIGSRSNFMFLVHN
                     ESVGSSFTSPRPFRVNAGAVHCYTISPDGTTKYLSELETGSEVLVLDSRGHARRATVG
                     RSKIESRPMLMIKAQIGDEIGGIIAQNAETIRFVRPNGHLISVTHLKKGDLVLAYTKP
                     ATGRHFGMEVDDEYIVEK"
     gene            4546..4707
                     /locus_tag="VLB45_01085"
     CDS             4546..4707
                     /locus_tag="VLB45_01085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04332.1"
                     /translation="MPPTNDKTVEFVDLCVICRQGITRGGLVFVDKKAYHSSCYAAFG
                     ERRGNVKPS"
     gene            complement(4726..6909)
                     /locus_tag="VLB45_01090"
     CDS             complement(4726..6909)
                     /locus_tag="VLB45_01090"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF012630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031012 - extracellular matrix [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HSD04333.1"
                     /translation="MVIVVPATLSIDAYAQDDSRIPSWIKTIIVAWAEGDISDTEFAN
                     AIEYLADNNILKISSEDTNEQLLNELEYLRAKNDVNKEELNILRTENEEYRIQILTLK
                     QNMAPKDATGYLSEANKKMQSDLSLLKAENKSYKNLLDSMTAENEAYQNKLNFLAAEN
                     EEYKIRINSLKQDKTSEEINQLKLEIDKYKNELEFLQTENAEYKNKINSILSDKKAEN
                     DIRQLKAELEKSQNEIDSLKIENNQYKNKVNSLITDTKIKDEINRLKTSITNYEKEIK
                     SVKDDNTQYKNKLGTLSTENSEYQDQINSLTYENTGYKNKLDSLISENSDYKNKLSSL
                     KDENAEYKNKINSLSVNKESVSKESESKLTSLKNEINQYKEKIKLLQTENEGFKKQSE
                     SLTIGDTKYKNEIEYLRAKNEVNQEELNILRTENEEYRIKLTLNMDGKFKPFSKLKDF
                     SYDKVEEISTDVKNGKYSASLGSEKSKEKSLSKAANSYSFLKGTKKTIYVEPLPEWGE
                     HGINSVNDAISFWENTAGVKFSRVEIAGNADIVVKRVKELSGQHHGLFMNNVIEVGIG
                     NTDCNNEWHSFDSNSISALMIHEFGHALGLEHNENPNDVMFPIISKASYASIEKNYFI
                     KTKQALFVPGCTLRDETPYSYNVSVDDKTHYFDVFVVPSKFQYDNYLKGNKFQYYSDE
                     GCFAINRLSYSGTCNGVANTGGLLIIIDENSEKTTATLNVKIQEK"
     gene            complement(7037..7240)
                     /locus_tag="VLB45_01095"
     CDS             complement(7037..7240)
                     /locus_tag="VLB45_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04334.1"
                     /translation="MVTSNTKSYFRSVEKSHRKYSVAIKKARSRKSIMTLYWRHKREH
                     EAILRKHLREEMAEINQLKKKIK"
     gene            complement(7289..8143)
                     /locus_tag="VLB45_01100"
     CDS             complement(7289..8143)
                     /locus_tag="VLB45_01100"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HSD04335.1"
                     /translation="MVLVWKPNSIVNADSKDLSEIGKDKVTLTVTSPPYHNAINYQEH
                     QSSTEWYRGTVGASLDSWIDEMRDVFSQVYKVTKPGGYCCIVIGNEIVEEKNKLPLPA
                     ILLVELTKKEIGWRFFEEIIWNKVTGGKKRFRVTVQHPYPTYYYPNIMHEQIIILRKP
                     PYYNKKDKKSKLKLNDLMKKEMANSVWHIAPVPPSYRKYHPAAFPEEIPYRLIQLYSN
                     VGDLVLDPFVGSGQTTKMARFLKRKYIGVDKSQKYVKIARKRTQEPQLLRAMQLVPYW
                     KKPEPIGV"
     gene            8206..8880
                     /gene="aroD"
                     /locus_tag="VLB45_01105"
     CDS             8206..8880
                     /gene="aroD"
                     /locus_tag="VLB45_01105"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HSD04336.1"
                     /translation="MANKTCVTLAESSPSKLRTILTKALRKSDYAEIRLDFMEPHKIP
                     LALNLVRKDLKRCVCTLRPKSEGGKFSSSEKNRTSILKLIAEYDPYLIDIEYNTIRKN
                     KNLQQYLKKTKTDLLVSWHDFKKTPNSSVLKNMLKKMNKFSKNIKIVTTARSIGDTAK
                     VLSLYKEISKKNNLIAFAMGDYGRMSRILCTQIGSPYTYVSLGKPIAPGQFSLDEVKS
                     IFKLKK"
     gene            8886..9707
                     /gene="aroE"
                     /locus_tag="VLB45_01110"
     CDS             8886..9707
                     /gene="aroE"
                     /locus_tag="VLB45_01110"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HSD04337.1"
                     /translation="MTKTYAVIGDPIEHSLSPAIHNAAFTFLGLDCTYIAYRIPKGEL
                     AGGIEALRKINISGFNVTIPHKVEMMKFLDDLDEVCKMIGATNTVDNEGGKFKGYNTD
                     MDGFLDPIKQRNLSCKDASVLLVGAGGAARAIVAGFAKEKASKITIANRTKEKSEEMV
                     RFATSLGLESEHTDLDSAGNNAGNYKFIVNATSVGMKNTGTPISTRAINKDSIVYDIV
                     YMPVDTELLEQSKKRGATIIYGWEMLLGQAMRSFEIWTKRKAPYEAMKLTLLGRF"
     gene            9709..10563
                     /locus_tag="VLB45_01115"
     CDS             9709..10563
                     /locus_tag="VLB45_01115"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HSD04338.1"
                     /translation="MPTVKAVMHGAVSIVNAIATGKGATLGISLNVEATIESSEGRGI
                     IFENNESGLSSRLIKNVIEKAVPKNDLERSKIRISLKSEIPSGYGLKSSSAISSVVSL
                     ACAKLFRPQIDDLEVLRHGVEASIESKVSITGAFDDACACYFGGFVVTDNHNLKIVKL
                     EKAPEDLTVVIFIPKSRKRGNVKKLRILDSVFAQAWNLASNSDYWNAMLLNGLATSTV
                     LDSDPRILTELIESGAISASVSGNGPSIAAVAKKENVSNVKKTFSSHDGSIILSDINN
                     KKAEVYEV"
     gene            10553..11821
                     /gene="aroA"
                     /locus_tag="VLB45_01120"
     CDS             10553..11821
                     /gene="aroA"
                     /locus_tag="VLB45_01120"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010195582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HSD04339.1"
                     /translation="MKCKIEKAKLSGTIACPPNKSYTHRAIFLSTLAKGKSQLDNVLL
                     SRDTIATIDACRKFGAQIKTDGASVTIESSGEIKPSSSEIDASNSGTTIRIAAAIASL
                     IENKTTLTGDSSLKKRPMQLLLDALEHLGASCTSTDGKPPITVKGKIAGGEVSIPGSV
                     SSQFITALMIAAPRTEKGITLNIEGELVSKPYLEATIATMKKFGAEVQVITPYKKYKI
                     PRQNYSSTTFTVPSDFSSMALLLSAAVLLGDSLTILASVGDLPQGDMAIISHLEKLGV
                     KVNIDGNRISVKSPQLLEGGKFDLSNTPDLLPAVAILALKTSKPIEIFNVKHARFKET
                     DRIAILASELAKIGIMVQEKEDGLILQAPRAPRGADLDSSDDHRLFMAFCIAGMHVGN
                     CTVSDPESVDVSYPSFIGDMNRVGAKILPN"
     gene            11860..12960
                     /gene="aroC"
                     /locus_tag="VLB45_01125"
     CDS             11860..12960
                     /gene="aroC"
                     /locus_tag="VLB45_01125"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HSD04340.1"
                     /translation="MMSSSSIGQRFVFTSFGESHGKCIGAVVDGCPAGLEIQEKDIQK
                     MLDLRKPGQSLVSTQRKEGDVVEILSGVFRGYTTGAPIAMIIWNQDQHSRDYEQLITK
                     PRPGHSDYPAWVKYKGFNDHRGGGRFSGRLTATFVMAGAIARKLLSTTLGIDTYSYTS
                     QIGKIKMNEQVTQKMKKEIYSNEVRCPDKKIAQKMRSAILAARKSGDSLGGVIESITT
                     NIPVGLGEPIFGSLESDLSKALYSIPAVKGIEFGSGFYGSTTSGSINNDEYLVKNGKI
                     VTKTNNAGGILGGLSNGMPIVLRVAFKPASSIAKKQNTVDIKTKKSASLVVTGRHDPC
                     VVPRAPPVVDSLVSVVLADHAIRSGLIPPVLR"
     gene            12964..>13286
                     /locus_tag="VLB45_01130"
     CDS             12964..>13286
                     /locus_tag="VLB45_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HSD04341.1"
                     /translation="MSEIDKLRDQMDKITLEMIKLLKDRAEISKQIGSLKSNLGLGIT
                     NEERESQLRTKVISLCKEIGLDEKIATMLLNFLLNESIKIQSTNKQTHLSIFLKARSL
                     EQQGK"
BASE COUNT         4232 a         2452 c         2646 g         3956 t
ORIGIN      
        1 aacgagtttg tagaaaaact agaaaatgcg ggagagttaa aacgcgttaa aacacaagtt
       61 gatgccgatt tagaaattgc agaaatttta agaagaacga tgtattcaaa cggccctgca
      121 ttattttttg aaaatgtaaa aaaccacacc atgccggttt tggcaaatgc ttttggttct
      181 ctgaaaagac ttgagatcgg tcttgaaact tcagacttta cacaaatagg tcagagaata
      241 gtcgacatga caaagatgga catcccatca gggctctttg ataaaattag aaaacttcca
      301 gagctttcaa agatgacaga cattgcacca aaattacaaa aaagcggtcc agtgacagaa
      361 atttttgaag atgcgccatc ttttgaaaag attccaattc taaaaacatg gcacaaggat
      421 gcaggaagat tcatcacttt tggccttgta gccacaagac atccagagac tggcataaga
      481 aatctcggag tctacagaat gcagatactt gacaagacac atgctctcat gcactggcaa
      541 aaacacaaga ggggagcaca tcactacgac ataaaaaaag acaaaggtga caagattgaa
      601 gttgccatag taataggtgg tgatcctgcc acggtatttt ctgcagtagc tccagttcca
      661 gaaggactag acaagtacct ttttgcaggc attgtaagaa aaactggaat aaagacagta
      721 aaatgcaaaa cagtggatct tgaagttcct gcaaatgcag agatggtcct tgagggatat
      781 gttgacccat cagatattag aatggaaggt ccatttgggg atcacactgg atattacact
      841 ccaccagaac catatccaac atttactcta actggaatca tgcgacgtga aaagccaatt
      901 tatcttacta cgatagttgg aaaaccaatt gtagaagatg cttttatcgg caaagtaata
      961 gagcgttcct ttttgccgct tatcaaaatg ttccagccag aagtcatgga tttttccatg
     1021 ccagctgcag gatggtttca gggaatggca atcatttcga ttaagaaaag atacccaggc
     1081 caagcaaaaa aagtaatgat gggtttgtgg ggacttggcc agcttgcatt aacaaaaatt
     1141 tttgttgttg tagatgatga cattaacgtg cacgacatga acgatgtcat atgggcaatg
     1201 acaacaagag ctgatgctgc ccgcgatgtc atgattgtag aaaatacgcc gactgatact
     1261 ttggatcccg catccccact tgtgaatctt ggatcaaagc ttggaataga tgctacacaa
     1321 aaaacaaggg aagaaggata tcaaagagag atacaagaag tagtaaaagt tgatgaagat
     1381 acaagaaacg ttgtggatgc caagtggtct agttacggaa tctagtgcat ttgtactaga
     1441 ttatagaaat tatctcgctc ttcgacttca aatccaattt gctttactgc gtggatgatt
     1501 ttgtttgcgg taagttccgt agaacgagcc cccgtacatg agacaacctc ttctcccaag
     1561 agtgtacctc caaagtcatc agcaccatat tgtagtgcag cctgtgccat gccaacacca
     1621 ttggttaacc aagatgactg gatatgtgga attagtccat catacattat tcttgaaatt
     1681 gcaatcattt ttagtaattg ataacctcct gcgggatata gtacggtttt ttcttcttgc
     1741 attagtgtgt tgtttggttc aaagctccat gggataaatg ccataaagcc tcctgtcttt
     1801 tcttgtagtt ttgcaatttt gacaaagtgt tctgcaatgt catgaggagt ttccacatgt
     1861 ccgtacatca ttgttgcaga agctggcagt cccatgctgt gtgcttcttc catgattttc
     1921 aaccattgtt cacttgagat tttttttgga ctgattacat ctttgacaga atctaccaag
     1981 atttctgcgc ccgcacctgg gactgattgt agtcctgctt cttttagtcg cgcaagcact
     2041 tcttgtgtac ttgatttttc aactcttgca atcatatcaa tttctgatgc tgacaatccg
     2101 tgaactccaa cctttgggaa tttttttcta attaatctaa acgcatcttc ataatactcg
     2161 attccaagtt ttggattgtg tccaccttga attaacactt gggtgattcc aaacatctcc
     2221 caagctgcct tgacacgtga ttctatttgt tcgatagata cagtgtatga ttcctcatgg
     2281 tctggcggtc tgtaaaatgc acaaaacttg caatcagtta tgcagacatt agtatagttt
     2341 agtatcatgt tatttacaaa cgatgctttt tttccgtaca attttcttgt catatatcct
     2401 gccacaagac ccatcagatg tacatcgttt gattccaaaa gtcgtataca gtcttctaac
     2461 tctggtcgtt ttccatcaag cgagttttca agaatatcgc caatctcaga agcataaagc
     2521 tcctttgtta tctggctcat tttgtttttg cttttgtttt atgctattta atcctttgag
     2581 aacatgtggt ggtgcaatcc actcgctatt tttaagtagg gtacaccaaa ggatatcttc
     2641 tatggtaaca gggctacaaa tcagactaaa tcgaattttg agatctggaa agatgctttg
     2701 cattccaatg gaccacggta tttcaagcgg tccgattaag ggactagagg atccccacaa
     2761 tgtcatttac acttgtcagc attatggatt aacgtcagtc atagtcaaca aaggaatttt
     2821 aaagacgcta ccaaaagttc cacaagtagg tgtactggtg catttttcag ctagcacatc
     2881 actttctaaa actccaaaca gaaagatgct ttcaggttca gtagaagaag ccgtgcgtct
     2941 tggagctgac ggagtgtcat tacacatcaa cataggcgga aaggaagaac cagaaatgct
     3001 agaacagctt ggaaaaatat ctgatgattg tcacaagtgg gatatgccac ttttagctat
     3061 gatgtaccca agaggcgaaa acatcaaaga tccacatgac ccagaaattg tagcccatgt
     3121 tgcaagaatt ggtgcggaga tgggtgctga tattgtaaaa actttgtaca ctggggattt
     3181 tgattcattt gcaagcatcg taaaaagcac gcctgttcca atagtgatag ctggcggtcc
     3241 aaaagcaaaa accgacaagg atgttttgga aatgacagag gaagcaatgc gggcaggtgc
     3301 aaaaggcgta acatatggaa gaaatatctt cgaacacaaa catccagata gaataaccga
     3361 ggctttggca ggcattatat ttagaaaaga aactgcaaag gaagcggcaa aacgacttga
     3421 caaaaagtag ggagctgata atatccccaa aggtgtcaag gggacagctt ggcaaatttc
     3481 ttgtagaaat cgagagagag ggaatcaaaa ctgttaatgt cgaccctcaa agcctaaagg
     3541 gaataaagac aaagcttaca accatacata gttctccaag tgcaaattat gtcatacttg
     3601 ataaaaaaac aacaaaacca aagggcaaaa agattggaat caaattcaag gttttatcta
     3661 atgtagatat tgaaaaaata ctagcagatg caaagacggg acttgatttt gtaattgtcg
     3721 aggtaaaaga ctggaaaatc atcccacttg aaaacataat tgcaaagctg cacaagttgc
     3781 acaccaagat atttgcaact gcaaacaatc cagaagaagt aagaaagatg ttttctattt
     3841 tggaaattgg tgttgatgga gtcatatttt caacaaattc tatcaacgag gtaagagaag
     3901 cactagtata ccttggaaca aaaagctttg agctcaagcc agccagggtt ttggaaatca
     3961 aagaggttgg aaatggagaa cgcgtatgtg ttgataccgc atcaatgctt ggaaggggag
     4021 aaggaatgct aattggcagc agatcaaact ttatgttttt ggtgcacaat gaatctgtcg
     4081 gttcatcttt tacttcacca aggccgttta gagtaaatgc cggtgcagtt cactgttata
     4141 ctatttctcc agatggaact acaaaatatc tttcagaatt agaaacaggc tctgaagtac
     4201 tggtgctaga ttcaaggggt catgccagaa gagcaacagt tggtcgctcc aaaatagaaa
     4261 gcaggccaat gcttatgatc aaagcacaga ttggagatga gataggcgga ataattgcac
     4321 agaacgcaga gaccatacgc tttgtcagac caaacggaca tttgatatct gtcacgcatc
     4381 tcaaaaaagg tgatctggta ttagcttata ccaaaccagc tactggaaga cattttggta
     4441 tggaagtaga tgacgaatac atagtggaaa aataagttct cacaggttta ttccatatga
     4501 tatttagcat gagctttaaa tcatgttgta aagagtaaga gttggttgcc accaactaat
     4561 gacaaaactg tagagtttgt agatttgtgt gtaatatgca gacagggaat aactcgtggc
     4621 ggtctagttt ttgtagataa gaaggcgtat cattctagct gttatgctgc atttggcgaa
     4681 agacgaggca acgtaaaacc ttcttgagtt gcaaactggt ttctgttatt tctcttgtat
     4741 ctttacgttt agtgttgcag tagttttctc agaattctca tcaattatta ttaataatcc
     4801 tccagtattt gcaacgccat tgcaagtacc actatagcta agacgattta ttgcaaaaca
     4861 accttcgtca gaatagtact ggaacttgtt tcctttcaga taattatcat actggaactt
     4921 agatggcact acaaatacat caaaataatg agtcttgtca tcaacactca cattataact
     4981 gtaaggcgtt tcgtcacgca gtgtacatcc tggaacaaac agagcttgtt ttgttttaat
     5041 aaaataattt ttctcaatag aagcatagct tgccttacta attattggaa acatcacatc
     5101 attaggattt tcgttgtgtt caaggccaag agcatgtcca aattcatgga tcataagagc
     5161 agagattgag tttgaatcaa acgaatgcca ttcattattg caatcagtat ttcctatacc
     5221 aacttcaatt acattattca tgaacagtcc atggtgttgc ccagaaagtt ccttcacacg
     5281 cttgacaaca atatctgcat ttccggctat ttctactcta gagaacttca cgccagcggt
     5341 gttttcccag aaacttatgg catcattaac agaatttatt ccatgttctc cccattcagg
     5401 caaaggctct acgtagatgg tcttttttgt tcccttaaga aatgaataag aatttgcagc
     5461 tttggacagc gatttttctt tagacttttc actgcctaag gatgcggaat atttgccatt
     5521 ttttacatct gtactgattt cctcaacttt atcataactg aaatctttta attttgaaaa
     5581 tggtttgaat tttccatcca tgttaagagt gagtttaatt ctgtattcct cgttttcagt
     5641 tcttaaaatg ttcagctctt cttgatttac ttcgttttta gctcgcaaat attcaatctc
     5701 gtttttatat ttagtatcac ctattgtcag agattcagat tgttttttga atccctcatt
     5761 ttcagtttgt agtagtttta ttttttcctt atattgatta atttcatttt tgagagaagt
     5821 tagcttactt tctgattctt tactgactga ttctttgttg acagataatg agtttatttt
     5881 gtttttgtat tcagcatttt catctttcaa ggaactcagt ttatttttgt agtcagagtt
     5941 ttcagatatc aacgagtcta gtttgttttt gtatccagta ttttcatatg tcaatgagtt
     6001 tatttgatct tgatattctg aattttctgt agacagtgtg cctagtttat ttttatactg
     6061 tgtgttatcg tcttttacag attttatctc tttctcataa ttagtaattg atgtttttag
     6121 gcgatttatc tcatctttta tttttgtatc tgttatcagg gaatttactt tgtttttgta
     6181 ttgattgttt tcgattttta gtgagtctat ctcgttttgg gatttttcaa gttctgcttt
     6241 aagttgtcgt atatcgtttt ctgccttttt gtcagacaat atggaattta ttttgttttt
     6301 gtattcagca ttttcagttt gtaagaattc aagttcattt ttgtatttgt caatttccaa
     6361 tttgagttga ttaatctctt cagatgtttt atcttgttta agtgagttta ttctgatctt
     6421 gtattcctcg ttttcagcag ccaagaaatt taatttgttt tggtatgctt cattttcagc
     6481 agtcatggaa tctagcaaat ttttataact tttattttcg gcttttagaa gactcagatc
     6541 actttgcatc tttttatttg cctcacttag ataacctgtt gcatctttag gtgccatatt
     6601 ttgttttaaa gtaagtattt gaattctgta ttcctcgttt tcagttctta aaatgttcag
     6661 ctcttctttg tttacatcat ttttagctcg tagatattct aattcattta gcaattgctc
     6721 gtttgtgtct tcactactta tttttaaaat attattatca gctaaatatt caatggcatt
     6781 tgcaaattca gtatctgaga tatctccttc agcccatgcc acaatgattg tttttatcca
     6841 gctaggaatt cttgaatcat cctgtgcata ggcatcaatt gaaagtgtcg ctggcactac
     6901 tataaccaaa ctcaacacga ttacaaaaaa caggaatcgc attagatgat cagatactta
     6961 gtttcgtatt aacctaatca tcatacctca cactatacac gaataggcat accaaaaatg
     7021 ggattaaaga gaagaattac tttatcttct ttttcagttg gtttatttct gccatttcct
     7081 ctcgcaggtg ttttctgaga attgcttcat gttcgcgctt gtgtctccag taaagagtca
     7141 ttatgctctt tcttgatctt gcttttttta ttgcgacaga atattttctg tggcttttct
     7201 caacacttcg gaaatagctt ttggtgtttg atgtaaccat aaattcaatg acagaatgta
     7261 tctagataac ctttgcgaat aattctgttt agacaccaat tggttcgggt tttttccaat
     7321 atggaacaag ttgcatcgct ctaagcaatt gaggttcttg cgtgcgtttt cttgcaatct
     7381 tgacatattt ttgcgatttg tcaacaccaa tgtattttcg ctttagaaat ctggccatct
     7441 ttgttgtctg gccactgccc acaaaaggat caagcaccaa gtctccaaca ttactgtaaa
     7501 gctgaataag cctgtacgga atctcttctg gaaatgctgc cgggtgatat ttcctatagc
     7561 ttggcggaac cggagcaatg tgccatacag agttggccat ttcttttttc atcaaatcat
     7621 tgagttttag tttgcttttt ttgtccttct tgttatagta aggtggtttc ctgagaatta
     7681 tgatttgctc atgcattatg tttggataat agtatgtagg gtatggatgt tgtactgtca
     7741 ctctaaaccg cttttttcca cccgtaactt tgttccagat aatttcctca aaaaatctcc
     7801 agccaatttc cttttttgtc agctcaacaa gcaatatagc aggaagggga agcttgtttt
     7861 tttcttcaac aatttcattt cctatcacta tgcaacagta cccaccaggc tttgtgacct
     7921 tgtaaacttg agagaaaaca tctctcatct cgtctatcca actatcaagg gatgcgccaa
     7981 cagtacccct gtaccactca gttgaacttt ggtgttcctg ataattgatg gcgttgtgat
     8041 atggtggcga agttacagtt agtgtgacct tgtcttttcc tatttcagaa agatcttttg
     8101 agtctgcatt aacaatggaa tttggtttcc acacaagaac cataatttgc ttgtctattt
     8161 attatttgaa caaaggctta attttacaat ccagcacata aaatcatggc aaacaagacg
     8221 tgtgtgacac tggcagaatc tagtccttca aagcttagaa ccattctaac taaagcgcta
     8281 agaaagtcag actatgcaga gattcgactt gattttatgg aaccacacaa gattccactt
     8341 gcgctgaatc tggtaagaaa agatctaaaa agatgcgtct gtacattacg accaaagtcg
     8401 gagggtggaa aattttctag cagtgaaaaa aacagaacat caatactaaa actgattgca
     8461 gaatatgacc cgtatttaat tgatatagaa tataacacaa tcagaaaaaa taaaaacttg
     8521 caacaatatc taaagaaaac aaagacggat ctgcttgttt catggcacga ttttaagaag
     8581 actcccaatt ccagcgtgtt aaaaaacatg cttaaaaaaa tgaacaaatt ttcaaagaac
     8641 atcaagattg taaccactgc aagatcaata ggagatactg caaaggttct gtcattgtac
     8701 aaggaaattt caaaaaagaa taacctaatc gcgtttgcca tgggcgacta tggcagaatg
     8761 tcaaggatac tctgcacaca gataggcagc ccctacacgt atgtgtctct tggcaagccg
     8821 attgccccag gtcagttcag tttagatgaa gttaaatcaa tcttcaagtt gaaaaaataa
     8881 caaacatgac aaaaacttat gcggtgatag gtgatccaat tgaacattca ctctcacctg
     8941 ccatacacaa cgccgctttt acatttctag gactggattg cacctacatt gcatacagga
     9001 taccaaaagg tgagcttgcc ggaggaattg aagccctaag gaagatcaat atctccggat
     9061 tcaatgtaac aataccacac aaagttgaga tgatgaagtt tcttgatgat ttggatgaag
     9121 tgtgtaaaat gattggggca acaaacacgg ttgataacga aggcggaaaa ttcaagggat
     9181 acaatacgga catggatgga tttttggatc caataaaaca aagaaaccta agctgcaaag
     9241 atgccagtgt gttgcttgta ggtgcaggag gagctgcaag agcaattgtg gcaggatttg
     9301 ctaaagaaaa agctagcaag ataactattg caaacaggac caaagaaaaa tctgaagaga
     9361 tggtgagatt tgcaactagt cttggattag aatcagaaca tactgattta gacagtgcag
     9421 gaaacaatgc aggcaattac aagttcattg taaacgcaac ttcagtcggc atgaaaaaca
     9481 ctggaactcc catatccaca agagccatca ataaagacag catagtgtac gatatagtat
     9541 acatgccagt tgatactgaa ttgcttgagc aatcaaaaaa acgcggtgct actatcatat
     9601 atggatggga gatgctcctt ggtcaggcaa tgcgttcatt tgagatttgg acaaaaagaa
     9661 aggcaccgta tgaggcaatg aagttgacgc ttttagggag attttgatat gcctacagta
     9721 aaagcagtca tgcatggggc agtctcaatt gtaaacgcaa ttgccacagg caagggtgca
     9781 acgcttggaa tttcacttaa cgtagaagct acaatagaat catcagaagg gaggggaata
     9841 atttttgaaa ataatgaatc cggcctgagc tcgcgtctta taaaaaatgt aattgaaaaa
     9901 gccgtaccga aaaatgatct tgaaagatcc aagattagaa tttcactaaa atcagagata
     9961 ccgtcaggat acggactgaa aagctccagc gcaatttctt cagtggtgtc tttagcgtgt
    10021 gccaaactct tcagaccgca gatagacgat cttgaagtct tgcgacatgg tgttgaggca
    10081 tcaatagaat ccaaagtcag catcacaggg gcatttgatg acgcatgtgc gtgctatttt
    10141 ggaggatttg tggttacaga caaccacaat ctaaagattg taaaattaga aaaggcgcct
    10201 gaagatctaa ctgtagtaat attcatacca aaatcaagaa agcgaggcaa tgtgaaaaaa
    10261 ctcaggatac tagacagtgt atttgcacag gcatggaatc ttgccagcaa ttcagattat
    10321 tggaacgcaa tgctgctaaa cggccttgca acatcgacag tacttgattc tgatcctaga
    10381 atccttactg aactaataga atctggcgcc atatctgctt cagtttcagg caacggtcct
    10441 tccattgcgg cagtagcaaa aaaagaaaat gtctcaaatg tgaaaaaaac gttttcctct
    10501 catgatgggt ctataatatt gtctgacatt aacaacaaaa aggccgaagt ctatgaagtg
    10561 taaaatagaa aaagcaaaac tatctggcac aatagcttgt ccaccaaaca aaagctacac
    10621 gcatcgtgca atatttttgt caacacttgc aaaaggaaaa agtcagctag acaatgtttt
    10681 gttatcaaga gatacgattg ctacaattga tgcatgcaga aaatttggtg cgcaaatcaa
    10741 aactgacggt gctagtgtta caatagaaag ctcaggcgaa ataaaaccgt caagctcgga
    10801 aatagatgca tcaaactctg gaaccacgat tagaatagca gctgcaatag catcactgat
    10861 tgaaaataaa actacattga ctggggattc tagtctcaaa aaaagaccca tgcaattatt
    10921 gcttgatgca ctagagcatc tgggtgcaag ctgcacttca actgacggca aaccgccaat
    10981 aactgtaaaa ggaaaaattg caggaggcga agtgtctatt ccaggttcag tatccagtca
    11041 gtttatcaca gcgttgatga tcgctgcacc tagaacagaa aaaggaatta cgttaaacat
    11101 agaaggtgag cttgtctcaa agccatactt ggaagctact attgccacaa tgaaaaaatt
    11161 tggagcagaa gttcaagtaa ttacaccgta caaaaaatac aagataccaa gacagaatta
    11221 ttcttctaca acatttacag tcccatcaga tttttcaagc atggcactgc ttctctcggc
    11281 agcagttctt ttgggagaca gtcttactat acttgcctca gtaggtgacc ttcctcaagg
    11341 agatatggct attatctctc atcttgagaa acttggtgtc aaggtgaaca tagacggcaa
    11401 taggatcagt gtaaaatcgc cgcagttatt ggaaggtgga aaatttgatc tatccaacac
    11461 accagaccta ttgcctgcag tggcaattct tgcactaaag acatcaaagc caattgagat
    11521 ctttaatgta aagcatgcaa gattcaaaga aacagacagg attgccattt tagcaagtga
    11581 gcttgcaaag attgggatca tggttcaaga aaaagaagac ggattgatac ttcaagctcc
    11641 cagagcacca agaggcgcgg atcttgattc gtcagatgat cacaggttgt tcatggcatt
    11701 ttgtatagct ggcatgcatg ttgggaactg tacagtgtct gaccccgaat ctgttgatgt
    11761 gtcttatcca agttttattg gagacatgaa tagagttgga gcaaaaattc ttccaaatta
    11821 gcatagaagg attataagca tctaacaaga aaatttaatt tgatgtcatc tagttctatc
    11881 ggacagagat ttgtttttac cagttttggc gaaagtcatg gtaaatgcat tggcgctgta
    11941 gttgatggct gcccagcagg tttagagata caggaaaagg acatacaaaa aatgcttgat
    12001 cttagaaaac ctggccagtc tcttgtttca acacaaagaa aagaaggcga tgtggtagaa
    12061 attttgtcag gagtcttcag aggatatacc actggggctc caattgcaat gataatttgg
    12121 aatcaagacc agcattctcg agattatgaa caattaatta caaaaccaag acctggacat
    12181 tctgattatc cagcttgggt aaaatacaaa ggattcaatg atcatagagg cgggggcaga
    12241 ttttctggca ggcttactgc cacgtttgta atggcaggag caattgcaag aaagctactt
    12301 tctacaacac ttggaataga cacctattct tacacttcac aaattggcaa gataaagatg
    12361 aatgagcaag taacacaaaa aatgaaaaaa gagatctatt caaacgaggt aagatgtcct
    12421 gacaagaaaa ttgcacaaaa gatgcgtagt gccatacttg cggcaagaaa aagcggagat
    12481 tcgcttggcg gagtaataga atccataaca acaaacatac cagtaggact aggcgagccc
    12541 attttcggat ctctggaatc tgatttgagc aaagcgctgt actcaattcc agcagtaaaa
    12601 ggaatagagt ttggttcagg attttacggt tccactacta gtggttccat aaacaatgac
    12661 gagtatctag taaaaaacgg aaagatagtt acaaagacaa acaatgcagg cggaatacta
    12721 ggtggacttt caaatggaat gccaattgtt ttacgagtgg ccttcaagcc tgcttcatcc
    12781 attgcaaaaa aacaaaatac tgtagatatt aaaaccaaaa aatcagcttc acttgtagtt
    12841 acaggaagac acgatccatg tgttgtgccg cgtgcaccac ccgtagtgga ttctcttgta
    12901 tcagtagtat tagctgatca cgccataaga agtggtctca tcccaccagt gcttagatag
    12961 aaaatgtcag aaattgacaa gctgcgcgac cagatggata agattacact tgagatgata
    13021 aaattactaa aggacagggc agagatctca aagcagattg gaagcctcaa aagcaacttg
    13081 ggccttggca taacaaatga agaaagagaa agccagctga ggaccaaagt aatttcactc
    13141 tgtaaagaaa tcgggcttga tgagaaaatt gccacaatgc ttttgaattt tcttttaaac
    13201 gaatcaataa aaatccaatc taccaacaag cagactcacc tttccatatt tttaaaagca
    13261 agatccttgg agcagcaggg aaaaaa
//
LOCUS       DATIDL010000024         2797 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrosopumilaceae archaeon isolate SMAG_U16342
            k141_2055175, whole genome shotgun sequence.
ACCESSION   DATIDL010000024 DATIDL010000000
VERSION     DATIDL010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935836
            Sequence Read Archive: SRR25158326
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosopumilaceae archaeon (soil metagenome)
  ORGANISM  Nitrosopumilaceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrosopumilales;
            Nitrosopumilaceae.
REFERENCE   1  (bases 1 to 2797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2797)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/04/2024 05:09:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,520
            CDSs (total)                 :: 1,474
            Genes (coding)               :: 1,474
            CDSs (with protein)          :: 1,474
            Genes (RNA)                  :: 46
            rRNAs                        :: 1 (5S)
            complete rRNAs               :: 1 (5S)
            tRNAs                        :: 43
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2797
                     /organism="Nitrosopumilaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2055175"
                     /isolate="SMAG_U16342"
                     /isolation_source="soil"
                     /db_xref="taxon:2202732"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..682
                     /locus_tag="VLB45_01135"
     CDS             <1..682
                     /locus_tag="VLB45_01135"
                     /inference="COORDINATES: protein motif:HMM:NF013795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HSD04342.1"
                     /translation="VNYLPVGSTTAVRYYATQALEAGIGFINAMPVFIASESKWQQMF
                     ADKKLPLAGDDVMSQLGATVVHKTLVKLFVDRGVLVDGTYQLNIGGDMDFFNMLDEER
                     LEDKRISKTSAVKAMADYDIPMRIGPSDYVDFIDNEKICYINIEGKYFGKIPIKLDLK
                     LKVIDAYNSAGIMIDGIRGLKVALDRKLSGPMTSISSYCFKHPPIQMPYNEAKAAFLE
                     FVEGKRDR"
     gene            779..>2797
                     /locus_tag="VLB45_01140"
     CDS             779..>2797
                     /locus_tag="VLB45_01140"
                     /inference="COORDINATES: protein motif:HMM:NF024573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HSD04343.1"
                     /translation="MVHIRKLEIFGFKSFGYKNTLVNLEPGLVAISGPNGSGKSNILD
                     AISFALGENSPKIMRVDKLRSLLHDVENKHQGTKIARVSCHFDNTDRKIPVDSNTVTI
                     TREMDEAGENIYYLNQKKMQRNHILDILEVANAGIHKLNIVQQGTITRISEFNAEERR
                     KIIEDIIGLAYFDEKKTESLKQLDEADRRLEIALARMDEIKKRIDELEAERNYQLRYE
                     FLERELGRFHAIQASNKIRDIESQKVSKERNLNVILSESKKLEEQRTLLKEEIKKLEE
                     EKQKFMEEVNAYNIAKAAIDTELSAAMQQSEAANSKIATNGRRLEQINSRLPEIELEI
                     NALHEKRSTLESQILQLKESIKSIEETKRSTNTDLSSVDSELHQVMKQQSQAATNRLK
                     VDEKIKGLTRSLNDAKLSLSKIESERTDIASKIEQNSARIKSFSTEKERLLSLEQKLQ
                     VVRAGHEATIHELKSRLANLTERRAKIEKDIEDNSLILEKASKAAAQYEAKIKVVKEV
                     MHEDYSIAKLKEDSKRLGIQGLVYEVLSWDKQYERPILAVGSDWLKAMVVNDFGTLLG
                     LAEFAQEKKLPKIRIIPLDAIPNSKVDCPKESGIMGVLSDYIKCDERFNALKNFIFGN
                     IMLVDSKESAYSISKKGYKAVTSDGQFFEADANAVVVDVDSKISKLTKI"
BASE COUNT         1075 a          469 c          558 g          695 t
ORIGIN      
        1 tgtaaattat cttccagttg gcagcacaac tgccgtaaga tattatgcta cgcaagctct
       61 tgaagcagga attggtttta tcaatgcaat gcctgtgttt atagcatcag aatcaaaatg
      121 gcagcaaatg tttgctgaca aaaaacttcc acttgcagga gatgatgtca tgagtcagct
      181 tggggctact gtagtacaca agactttggt gaagcttttt gtggaccgtg gggttttggt
      241 tgacggtaca taccagctta acatcggagg tgacatggac tttttcaaca tgttagacga
      301 agaaagactg gaagacaaga gaataagcaa gacaagtgcg gtaaaagcaa tggcagacta
      361 tgacattcct atgagaatag gtcctagtga ttatgttgat tttatagaca atgaaaaaat
      421 atgctatatc aacatagaag gaaaatactt tggcaaaatt ccaatcaagc ttgatctgaa
      481 actaaaggta attgatgcat acaacagtgc tggtataatg attgatggaa tacgtggtct
      541 caaagttgca ctagatagaa agctttctgg acctatgact agcatctcat catattgctt
      601 taagcatcct ccgatacaaa tgccatataa tgaagcaaag gcggcttttt tggaatttgt
      661 agaaggaaag cgagacagat agatatacga agtaaaaatc cgcattagaa taattgttag
      721 cctagtcaat gaaacttgtc aatattcggt tatttcgaat accttgacat cgagactatt
      781 ggttcatatt cgtaagttag agatttttgg cttcaaatcg tttggatata aaaataccct
      841 agtaaatctt gaacctggcc ttgtggcaat atctgggcca aacggatcag gaaaaagcaa
      901 cattcttgat gccataagtt ttgcacttgg cgaaaactca ccaaagataa tgcgtgttga
      961 taaattgcgc tctcttttgc atgatgtgga aaacaaacac caagggacaa agatagcacg
     1021 tgtaagctgt cattttgata acactgatag aaagatccct gtagattcaa acacagtaac
     1081 aataactagg gaaatggacg aggctggaga aaacatctac tatctgaacc agaaaaagat
     1141 gcagcgaaac cacattcttg acatactcga agttgcaaat gccggcattc acaaattgaa
     1201 catagtgcaa cagggtacga ttacaagaat ttctgaattt aatgcagaag aaagaaggaa
     1261 gattatagag gatatcatag gccttgctta ttttgacgaa aaaaagacag aatctctaaa
     1321 acagcttgat gaggcagata ggcgtcttga gattgccctt gctagaatgg atgagataaa
     1381 aaaacgcata gatgaacttg aagccgaaag aaactatcaa ctgcgatacg agtttttgga
     1441 acgtgaactt ggcagattcc atgcaatcca agcctcaaac aagatacgtg atatagaatc
     1501 tcaaaaagtc tcaaaagaaa gaaacctgaa cgtcatcttg tctgaatcaa agaaactgga
     1561 agaacagcgt actttgctaa aagaagaaat caaaaaactg gaagaagaaa aacaaaagtt
     1621 tatggaagaa gtaaacgcat acaatattgc aaaggctgct attgatactg aacttagcgc
     1681 agcaatgcaa cagtcagaag cagcaaatag caagattgca acaaacggaa gaagattaga
     1741 acagataaac tctagattgc cagaaattga attagagatc aatgcattac atgaaaaaag
     1801 atctacgcta gaatcacaaa tcttacaatt aaaagaatca ataaagtcga ttgaggaaac
     1861 aaaaagatct acaaatacag atctttcttc agtagattct gaacttcatc aagtaatgaa
     1921 acaacaatca caagctgcaa caaacagact caaagtggat gaaaaaataa aaggccttac
     1981 aagatctctt aatgatgcaa aactatctct gtcaaaaatt gaatctgaaa gaaccgatat
     2041 tgcaagtaaa atagaacaaa actctgcaag aataaaatca ttctcaacag aaaaagaaag
     2101 attgttatca ttagaacaaa aattgcaagt tgtcagagct ggtcatgaag caacaatcca
     2161 tgaactaaaa tcaaggcttg caaacttgac agaaagacgc gcaaaaattg aaaaagatat
     2221 cgaagataac tctctcattt tagaaaaagc aagcaaagct gcagctcaat atgaagcaaa
     2281 gataaaagtt gtaaaagaag tcatgcacga agactattcc atagcaaaac taaaagaaga
     2341 ttccaaaaga ttgggcatac agggactcgt ctatgaagtt ctctcatggg acaagcagta
     2401 tgaaagacca attcttgctg taggttctga ttggctcaag gctatggttg taaatgactt
     2461 tggtacattg cttggattgg cagaatttgc acaagaaaag aaacttccaa aaatcagaat
     2521 aattcctctg gacgcaattc caaattccaa agtggactgc ccaaaagaat ctggaataat
     2581 gggcgtatta tcagactaca taaaatgcga tgaacgattc aacgcactaa agaactttat
     2641 ttttgggaac ataatgcttg tcgattcaaa agaatctgcc tactctatat caaaaaaagg
     2701 atacaaggca gtaacatctg atgggcaatt ctttgaagct gatgcaaatg cagtggtagt
     2761 tgatgttgat tcaaagatat caaaactaac aaagata
//
LOCUS       DATIDL010000025         3355 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrosopumilaceae archaeon isolate SMAG_U16342
            k141_1652359, whole genome shotgun sequence.
ACCESSION   DATIDL010000025 DATIDL010000000
VERSION     DATIDL010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935836
            Sequence Read Archive: SRR25158326
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosopumilaceae archaeon (soil metagenome)
  ORGANISM  Nitrosopumilaceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrosopumilales;
            Nitrosopumilaceae.
REFERENCE   1  (bases 1 to 3355)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3355)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/04/2024 05:09:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,520
            CDSs (total)                 :: 1,474
            Genes (coding)               :: 1,474
            CDSs (with protein)          :: 1,474
            Genes (RNA)                  :: 46
            rRNAs                        :: 1 (5S)
            complete rRNAs               :: 1 (5S)
            tRNAs                        :: 43
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3355
                     /organism="Nitrosopumilaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1652359"
                     /isolate="SMAG_U16342"
                     /isolation_source="soil"
                     /db_xref="taxon:2202732"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..264)
                     /locus_tag="VLB45_01145"
     CDS             complement(<1..264)
                     /locus_tag="VLB45_01145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04344.1"
                     /translation="MNKKILAGAISAGVIAIVIVAVFLSTQTTMLQKNEKLGLYILTP
                     SSTPTLTDLKEAYTEAVSAGIGRSNVYLFWPTLEPQQGQYDWKI"
     gene            complement(333..2990)
                     /locus_tag="VLB45_01150"
     CDS             complement(333..2990)
                     /locus_tag="VLB45_01150"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016939417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HSD04345.1"
                     /translation="MTDFSQIENSIIEVKKRDGRVLNFQKDKISNAVYKALVACNRPD
                     HSLADKLAEGVMMKLLNQGFAASAPPSVEDVQDMVESTLIDEGLGEIAKAYILYRHER
                     RKIREEKMKVLNAKSLDSVAKKFDLNCLRVLASRYLMRNNKGEIIESPSEMFERVAIL
                     VGLADVLKDNSVFDGAGGHTQNIDEAKKYLEKLDDFNFKFKIGKYYLNKWHFRGLISH
                     YIDLAKNGQMKASIKELLTKLAAKQFDQYADRIAEYYEMMVTQDFLPNSPTMMNAGGR
                     LGQLSACFVLAMPDDMEQIMKSSSDAALIFKSGGGVGINYSDLREEGDMVASTSGVAS
                     GPVSFMNIINTVTEVVKQGGKRRGANMGIIEAWHPDIEKFITAKTKAGVLENFNVSVG
                     VWEDFWHSLVNTSDGKFMLRSPRDKSPIREVNAHQLIDLIALSAWKSAEPGLIFFDLI
                     NKYNVFAKARGEPLRATNPCGEQSLYPYESCNLGSINLANFVKRKADGQYEFDWQRYE
                     ETIRKTTRFLDNVIDVNNYPIPEIDKASKESRRIGLGVMGVADLLFKLRIPYNSQEGY
                     EFQEKLAEALSYYSMEESVALAKTRGAFPLCAKTEYPEGKVPISGYYEKPKSSHYYDW
                     DALIEKIQKHGIRNVLTSTVAPTGTLSMIADCSNGMEPMFALVFEKRVTVGRFFYTNK
                     IFESVLKEHGLYNDQILEKIANNYGSVKGIQEIPEWIQKIFVTAMDIHWADHLMAQAV
                     WQKWIGNAIAKTINMPNDVTADDVKASYLLAHELGLKGITVFRDGSRHEQVLHMTGDN
                     VQKTFQVTPSTYLTDYISKSLTNAYLKSQVERALQIKIPDTEITQQKNSLTPEQIEER
                     LCPTCKNNLVFAEGCNICIECGYSGCTSG"
BASE COUNT          913 a          664 c          591 g         1187 t
ORIGIN      
        1 aattttccaa tcatactgtc cttgttgtgg ctctaacgta ggccagaaaa gatagacgtt
       61 acttcgaccg attccagcag atacagcttc cgtatatgct tcttttagat ctgtaagtgt
      121 tggtgtagat gatggagtta gtatgtataa accaagtttt tcattttttt gcaacatagt
      181 ggtttgtgtc gataagaata ctgctacaat aacaatagct attacacctg cagatatagc
      241 accagcaaga atttttttat tcaataaagc ttacactgat gattgatagt aaaaaagttg
      301 tgttcaagta tttatgaata aaagttggcg atctatccag acgtacagcc actatatccg
      361 cattctatac agatgttaca tccttctgca aagactaggt tattcttaca tgtaggacat
      421 agtctttctt caatctgttc tggtgtaagt gagttctttt gttgtgtaat ttccgtgtca
      481 gggattttga tctgtaacgc tctttcaact tgtgatttca agtaagcatt tgtaagactt
      541 tttgagatgt aatctgtcag atatgtactt ggtgttactt ggaatgtttt ttgcacgttg
      601 tcacctgtca tatgtaacac ttgttcatgt cttgaaccat ctctgaatac tgtgattcct
      661 ttcagtccaa gttcatgtgc aagaagatat gatgctttga catcatctgc agtaacatca
      721 tttggcatgt tgattgtttt tgcaattgca tttccaatcc acttttgcca gacagcttgt
      781 gccatcaggt gatctgccca gtgaatgtcc attgcagtga caaatatttt ctgtatccat
      841 tctggaattt cttggatgcc ttttactgat ccatagttat ttgcaatttt ttctagtatt
      901 tgatcattgt agagaccatg ttcttttaga acagactcaa agatcttgtt agtatagaag
      961 aatctaccca ctgtaacacg cttttcaaat acaagtgcaa acattggttc cattccgttt
     1021 gaacaatcag caatcattga aagcgttcct gttggtgcta ctgtagatgt taacacattt
     1081 ctgattccgt gcttttggat cttttctatt agagcgtccc aatcataata gtgtgaactc
     1141 tttggcttct cataataacc agatattgga acttttcctt ctggatattc tgtttttgca
     1201 catagtggga acgcacctcg tgtctttgca agtgccacac tttcttccat tgaatagtag
     1261 ctaagtgcct ctgctagttt ttcttggaac tcatatcctt cctgtgagtt gtatgggatt
     1321 cgtaatttga atagcaagtc agcaactccc atcacaccca atcctattct acgagattct
     1381 tttgatgcct tgtcaatctc tggaattggg taattattta cgtcaattac attatctagg
     1441 aatcttgttg tctttctgat tgtttcttca tatctttgcc agtcaaactc gtattgacca
     1501 tctgcttttc ttttaacaaa atttgcgagg ttgatggaac ccaaattgca ggattcatac
     1561 ggatacagac tttgttctcc acatggatta gttgcacgta atggttctcc tctagcttta
     1621 gcaaatacat tgtatttgtt gattagatca aagaatatca atccaggttc agcactcttc
     1681 caagctgaga gtgcaatcag atcaataagc tgatgtgcat ttacttcacg tattggtgat
     1741 ttgtcacgtg gactgcggag catgaatttt ccatcactgg tatttacaag tgagtgccag
     1801 aaatcttccc aaacaccaac gcttacgtta aagttttcta aaactcctgc ttttgttttt
     1861 gcagtaataa atttttcaat atcaggatgc catgcttcaa tgattcccat gtttgcacct
     1921 ctacgttttc caccttgttt gacaacttca gtcactgtgt taatgatatt cataaatgaa
     1981 acaggacctg atgcgacacc tgatgtagat gcaaccatat caccttcttc tcgtaaatca
     2041 gaatagttga ttcccactcc tccaccagac ttgaatatta gtgctgcatc ggaggaagat
     2101 ttcattattt gttccatgtc atctggcatt gcaagtacaa agcaagctga caactgtccc
     2161 agtcttccac ctgcgttcat cattgtggga gagtttggta gaaagtcttg agttaccatc
     2221 atttcatagt attctgcaat cctgtctgca tattgatcaa attgctttgc tgcaagcttg
     2281 gtaagtaatt ccttaatact tgctttcatc tgtccattct tggctagatc aatataatga
     2341 gaaattaatc ctctaaagtg ccatttattg agatagtact tgcctatttt gaatttgaag
     2401 ttaaagtcgt cgagtttttc aaggtatttt ttggcttcat caatattttg tgtatgtcca
     2461 cctgccccat caaaaacgga gttgtctttt agtacatctg caagtccaac caaaatggca
     2521 acgcgttcaa acatctcgct tggactttcg attatctccc ccttgttgtt acgcataagg
     2581 tatctggatg ctaggactct caaacaatta agatcaaatt tctttgctac gctatcaagc
     2641 gactttgcat taagaacttt catcttttct tctcttatct ttcttcgttc gtgtctgtat
     2701 aggatgtagg ctttggcaat ttcacctaat ccctcatcta taagggtgga ttctaccata
     2761 tcctgcacat cttccacgct tggcggagcc gatgcagcaa atccttggtt taaaagtttc
     2821 atcattactc cttcagctag tttgtcggcc aaactatgat ctggtctatt acaagcaaca
     2881 agtgccttgt atacagcatt tgaaattttg tccttttgga agtttaacac tcgtccatcg
     2941 cgctttttaa cttcaatgat cgagttttct atctgtgaaa agtctgtcaa acgtatcccc
     3001 gtaaccgtaa gaaaatagag tataataaac tctctggcaa aaaatatttt tctaaaattg
     3061 atttttgtat caattttgtt gacaccgtta caattatcat agtattggat cagaatgatc
     3121 ccaaataaaa accaaaaaaa tttaataaaa atttcagaat aatttgatcg catttttcag
     3181 ttttttttaa aaaagagcca gaatagaagg agcaaatgaa aacttgcgac gattaacgca
     3241 aggttatgcc aatatatatg gagagttgca ggtaggacat ttgtctccga tcttttcgtc
     3301 ttttagtcca caatttccgc agataggaac ctttctgagt ggcctgaaag aacct
//
LOCUS       DATIDL010000026         2651 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrosopumilaceae archaeon isolate SMAG_U16342
            k141_2058446, whole genome shotgun sequence.
ACCESSION   DATIDL010000026 DATIDL010000000
VERSION     DATIDL010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935836
            Sequence Read Archive: SRR25158326
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosopumilaceae archaeon (soil metagenome)
  ORGANISM  Nitrosopumilaceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrosopumilales;
            Nitrosopumilaceae.
REFERENCE   1  (bases 1 to 2651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/04/2024 05:09:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,520
            CDSs (total)                 :: 1,474
            Genes (coding)               :: 1,474
            CDSs (with protein)          :: 1,474
            Genes (RNA)                  :: 46
            rRNAs                        :: 1 (5S)
            complete rRNAs               :: 1 (5S)
            tRNAs                        :: 43
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2651
                     /organism="Nitrosopumilaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2058446"
                     /isolate="SMAG_U16342"
                     /isolation_source="soil"
                     /db_xref="taxon:2202732"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1195
                     /locus_tag="VLB45_01155"
     CDS             <1..1195
                     /locus_tag="VLB45_01155"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HSD04346.1"
                     /translation="YGDPLLKDQDLVIEKFVSGLSLPTTMSFVGDDLLVLEKNTGKVR
                     LVRDGILQEKEILDLSVSNNSERGLLGITTENSTIYLYLTESTGDGKTPLGNRIYQYT
                     WNGNELVNPILIKDLPATPGPNHDGGAMVTGLYGDVYAVIGDLNRNGVLQNYPSGEPD
                     DTSVIIRIIPDDVYYAIGVRNSFGLAIDPVTGNLWDTENGQNDFDEINLVLQNFNSGW
                     EKIMGPATEDDLSALPGFDAYVYSDPEFSWQEVVAPTALSFIDSDNLKAYQNSLFVGD
                     CNYGNLYKFELNENRDGFVFTDSNLSDNVANANESLEEIVFGTGFGCITDMEIGPDGF
                     LYIVSLSDGVIYKITPKTVTIMDKEIGFGEPPIVPIEYPIYGIVAALIIIGIIYLVKS
                     KNKKS"
     gene            complement(1204..1518)
                     /locus_tag="VLB45_01160"
     CDS             complement(1204..1518)
                     /locus_tag="VLB45_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010194429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04347.1"
                     /translation="MALFGVFSIHSPEACPLNNHANRLVAMKILEKMNNVKKKYRIKE
                     IVGFYASVLEHEFVVILDAQDAHDVEKMAIEVGLSKFNTLKIVPLNEFAHITKMWKGM
                     EK"
     gene            1611..1710
                     /locus_tag="VLB45_01165"
     tRNA            join(1611..1650,1666..1710)
                     /locus_tag="VLB45_01165"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1647..1649,aa:Leu,seq:caa)
     gene            2169..2540
                     /locus_tag="VLB45_01170"
     CDS             2169..2540
                     /locus_tag="VLB45_01170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04348.1"
                     /translation="MEDLFGILDKLSYLGIFLLLIAMNASPILMPPTWIVLASFYNVD
                     PALDVLALSAVGATGALIGRIILMYISRFFRRFIGKERVSNLDLLADFMKKKKYGFLS
                     ASFLFAITPLPSNLLSFVDFY"
BASE COUNT          772 a          478 c          525 g          876 t
ORIGIN      
        1 ttatggggat ccgcttttaa aagatcagga tctagtaatt gagaaatttg tttcagggct
       61 ctcattgcct acaacaatga gttttgttgg agatgatctg ctggttcttg aaaaaaacac
      121 cggaaaggta cgtctcgtaa gagatggtat cttacaagaa aaagaaattc ttgatctgtc
      181 agtaagcaac aacagtgaac gaggattact tggcattact acggaaaatt ctactatcta
      241 tttgtacttg acagaatcta ctggtgatgg gaaaacgccg cttggaaatc gcatatatca
      301 atatacttgg aatggaaatg aacttgtaaa tccaattctg ataaaagatc ttccagctac
      361 acctggacct aaccatgatg gtggtgcaat ggttacagga ttgtatggcg acgtttatgc
      421 cgttattggg gatttgaata gaaacggtgt gctgcaaaac tatccaagcg gtgaaccaga
      481 tgacacaagc gtgattattc gcatcattcc tgatgatgtc tattatgcga taggagttag
      541 aaacagcttt ggtctggcaa tagatccggt tactggcaat ctgtgggata cggaaaatgg
      601 ccaaaacgat tttgatgaga tcaaccttgt tttacaaaac tttaacagcg ggtgggaaaa
      661 aatcatgggt ccggcaactg aagatgatct ttcggcattg cctggttttg atgcatatgt
      721 gtacagcgat cctgagttta gctggcaaga agttgttgca cccacggcat tgagttttat
      781 tgattctgat aatttgaaag cataccagaa cagcctgttt gtgggtgatt gcaattatgg
      841 caacctatac aagtttgaat taaatgaaaa ccgtgacggg tttgtcttta ccgactctaa
      901 tctgtctgat aacgtggcca atgcaaacga atcgctagaa gaaatcgtgt ttgggacggg
      961 atttggctgt ataacagata tggaaattgg gccagacgga tttttgtaca ttgtgtctct
     1021 ttcagatggc gtaatttaca aaataacgcc aaaaacagta acaattatgg ataaagaaat
     1081 tggttttggt gaaccgccta ttgtgccaat agaatatcca atttatggta tagttgctgc
     1141 gttaataatt ataggtataa tctatcttgt aaaatccaaa aacaaaaaat cttgatcaga
     1201 ttattacttt tccattcctt tccacatctt ggtgatgtgg gcaaattcat ttaatggcac
     1261 aatctttaat gtgttaaatt tggacaaccc aacctcgatg gccatcttct ctacgtcatg
     1321 tgcgtcctgt gcatccaata tgactacaaa ctcgtgttca agaactgagg cataaaatcc
     1381 aactatttct tttattctgt attttttctt cacattgttc attttctcta aaatcttcat
     1441 tgcaaccaat ctgtttgcgt gattgttaag cggacatgct tctggactgt gaattgagaa
     1501 aactccaaac aacgccatat acacccactt ttcttttcta tattaaaacc aactgtcttt
     1561 tgcaaaacta actcgtttca gttatataat atgggcttga acttcacctt gcagaggtac
     1621 cgaagcccgg ccaaccgaga aggactcaag atcatggcat gggaaatcct ttcccttagg
     1681 ggttcgaggg ttcaaatccc tccctctgca tttttgttta gttttttctg tcgtctttgt
     1741 ctctgcttac gatttggttc atcaaaaccg cggtgatttt ctcccatgat aatagtatgt
     1801 gtcacatctt tttcaaaatt ttcattacaa actagaaacc agtatgaaaa ttattttgtc
     1861 ctaaactttg agcatttgta cttcacgaac tgaatgtcga gagagatctt catgaagcaa
     1921 ggcatgtgat tcttctgtaa aaaccagatt gcatctataa catttccaag cctttgtgct
     1981 cacatttatt aatatgtgct tttttcctta taaggatatt gtacgttttg tccattttct
     2041 tggataactg cgatctaact gatgatatct tggaattttt agtgcaattt gttgtcattt
     2101 gtgaattttt actagaggga ttcttgcaaa catgaatcaa ttaaagggtc ttgtaattta
     2161 gaaaaccaat ggaagacctc tttggaatac ttgacaagct gtcgtatctt gggatctttc
     2221 ttttgctaat tgcaatgaat gcatcgccaa tattgatgcc cccaacatgg attgttcttg
     2281 catcatttta caatgtagat cctgcactag acgtacttgc attgtctgct gtcggagcaa
     2341 ctggtgcact aattggaaga atcatcctga tgtatattag cagattcttt cggagattta
     2401 ttggaaaaga aagagtgtca aatttagatt tgcttgcaga ctttatgaag aaaaaaaaat
     2461 acggttttct ttccgcatca tttctttttg ccataactcc actaccaagc aatttgttgt
     2521 catttgtgga tttttactag agggattctt gcaaacatga atcaattaaa gggtcttgta
     2581 atttagaaaa ccaatggaag acctctttgg aatacttgac aagctgtcgt atcttgggat
     2641 ctttcttttg c
//
LOCUS       DATIDL010000027         4087 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrosopumilaceae archaeon isolate SMAG_U16342
            k141_2058664, whole genome shotgun sequence.
ACCESSION   DATIDL010000027 DATIDL010000000
VERSION     DATIDL010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935836
            Sequence Read Archive: SRR25158326
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosopumilaceae archaeon (soil metagenome)
  ORGANISM  Nitrosopumilaceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrosopumilales;
            Nitrosopumilaceae.
REFERENCE   1  (bases 1 to 4087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/04/2024 05:09:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,520
            CDSs (total)                 :: 1,474
            Genes (coding)               :: 1,474
            CDSs (with protein)          :: 1,474
            Genes (RNA)                  :: 46
            rRNAs                        :: 1 (5S)
            complete rRNAs               :: 1 (5S)
            tRNAs                        :: 43
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4087
                     /organism="Nitrosopumilaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2058664"
                     /isolate="SMAG_U16342"
                     /isolation_source="soil"
                     /db_xref="taxon:2202732"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1318
                     /locus_tag="VLB45_01175"
     CDS             <1..1318
                     /locus_tag="VLB45_01175"
                     /inference="COORDINATES: protein motif:HMM:NF014516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HSD04349.1"
                     /translation="RKSELVRYVKTAIASLQKDVLEKERKLSDCEGCFDHLFSKKEIE
                     TQMEKIEADLESTRNEIRQLSEKVGNLGGQEELAAKLKLKDGKCPVCDSVVDNLKPSF
                     QKEHIKEEITKMREKIGSLTKAEKETVLDKRELEQKYQNAIKAESTLKTHAISNPKDL
                     VQLKEEIGKLKSNIEKISINIVSSGNLVHLAIDSHSQTLLETISLLQEETKGFDLDEF
                     KNLKISLDQARKSLSGIDQQLGAIAQKILDAKKQQEKISKVLGELAFVKQYVSELDKI
                     RSQIFNRDGPVATSLRSWALNLISLKASEYLAAFNVKIQRVSLVEKTRDVGISCYSGN
                     TVLDLESLSGGEKVSVALALRLGMAHILGASNLNFIILDEPTTHLDEERRKSLVSVLS
                     QAFEANLNSISQFLIITHDSEIFENSNVDMIYNFESTPEGTKVTTL"
     gene            complement(1315..1803)
                     /locus_tag="VLB45_01180"
     CDS             complement(1315..1803)
                     /locus_tag="VLB45_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HSD04350.1"
                     /translation="MEVIPNSRLELLSEMEERYEKKDKEYFVQLLDHKDYVIRTRAVC
                     ILVDFGGEEMISHVSKVLKNDSNELVRHEAAFSLGQMCFRSGIKPLEDAVRNDPSFFV
                     RHEAAIALGVIGSQEAKETLQKALTDSSEPVRESAVVALSNLHFMEKLSKNERFAKLT
                     GG"
     gene            1843..2301
                     /locus_tag="VLB45_01185"
     CDS             1843..2301
                     /locus_tag="VLB45_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04351.1"
                     /translation="MSRCRYWHLTIEELKKVNYDQKKVLVWEIKCSKDDSGTHFGVFC
                     YRNGTPWDYKSVQGIVFYYNMIPLDEVKKITEFLKGKFGGEEKEKGERVFLVDSRVIY
                     SPIEIADLATEIGNKFETSVEISVELENFSPQEQEQSNFPSSKLLPIPGK"
     gene            2336..3133
                     /gene="tatC"
                     /locus_tag="VLB45_01190"
     CDS             2336..3133
                     /gene="tatC"
                     /locus_tag="VLB45_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HSD04352.1"
                     /translation="MDEIKQIGPHLEELRKRLIRMVLAVVIITAFVLTFHLYAVDYNG
                     MTLYYPLPDPLNNIAAQLTLEMKNQLVPETVQLIQTAPGQAFFAQFYVAALLGIVFGM
                     PVIVKELVSFVSPALGKKETRIIRSMTIPAIGLFITGCVFSYFFVTPYILEFLYTYGE
                     ASGLVTFLNVVDFITFVLQFLLAFGISFQLPIVMYAVSASGLADEKFWRKNIRYAIVI
                     IVIFGAIITPDGSGITMWFVAGPMIGLYLLGMIFTERKGRQIATLKS"
     gene            3155..3337
                     /locus_tag="VLB45_01195"
     CDS             3155..3337
                     /locus_tag="VLB45_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007550412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HSD04353.1"
                     /translation="MPVAGTEWIWIIVAIGVLIFGAKKIPELARTFGKAKGEYEKGKI
                     EADKELKEFKEKNDPK"
     gene            3375..>4087
                     /locus_tag="VLB45_01200"
     CDS             3375..>4087
                     /locus_tag="VLB45_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1246 domain-containing protein"
                     /protein_id="HSD04354.1"
                     /translation="MIRRSEIQEIVSGYSDLTVGTLGSHSALEIMDGAKDENLPTLVV
                     CQRGRETPYKRFSRIADEIVLVNKFAEMLSPKMQSKLRASNTVIIPHRALTAYLGYKR
                     IENNFKVPIFGNRALFQAEERSHRKNQYYLLEKARIKHPKLFKNPKDIDRPAIVKVQE
                     KSRKLERAFFNVSSFSDYKDKTGTKIRHGLISKEDLKIASIEELVIGTYFNFNYFHTP
                     ISDDVDFLGIERRLQTNLH"
BASE COUNT         1382 a          742 c          804 g         1159 t
ORIGIN      
        1 aagaaaatct gagcttgtaa gatacgtcaa aactgcaata gcatcactac aaaaagatgt
       61 tttagaaaaa gaacgaaagc tttccgactg tgaagggtgc tttgatcatc ttttctctaa
      121 aaaagaaatt gagacgcaaa tggaaaaaat tgaagctgat ctagagtcga cacgaaatga
      181 aatcaggcaa ctgtcagaaa aggttggaaa ccttggaggc caagaagaac ttgcggcaaa
      241 actaaagcta aaagacggca aatgcccagt gtgtgattcc gtagtagata acctaaagcc
      301 gtcattccaa aaggagcaca tcaaagagga aattacaaag atgagagaaa agattggctc
      361 tctcacaaag gctgaaaaag agacagtttt ggacaagcgt gaacttgagc aaaaatacca
      421 aaacgccatc aaggcagaaa gtacgctaaa gacccatgcc atctcaaacc caaaagacct
      481 agtgcaacta aaggaggaaa ttggcaaact aaaaagtaac attgaaaaaa tttcgataaa
      541 cattgtttct tccggaaact tggtgcatct tgctatagat tcacattctc aaactctgtt
      601 ggaaacaatt tctttgctgc aagaagaaac aaaaggattt gatctagatg aattcaaaaa
      661 cttgaaaatc tcgcttgatc aggcacgaaa atctcttagc ggtatagatc aacagctagg
      721 tgcaatagca caaaagatac ttgatgcaaa aaaacaacag gaaaaaatct caaaggtttt
      781 gggtgaactt gcttttgtaa agcaatatgt ttcagaatta gacaaaatac gcagtcaaat
      841 tttcaacaga gatggaccag tagcaacaag tctgcgttct tgggctctaa atttgatttc
      901 actaaaggct tcagaatatc ttgcggcatt taatgtaaaa attcaaagag tttctcttgt
      961 agaaaaaaca agagatgttg gaatttcttg ctattctgga aacaccgtct tggatcttga
     1021 atcacttagt ggcggagaaa aagttagtgt ggcacttgca ctacgacttg gcatggcaca
     1081 catacttggt gcatcaaatc taaacttcat cattttagat gaaccaacaa cacatcttga
     1141 tgaagaacga agaaaatcac ttgtcagcgt tctgtctcaa gcctttgagg caaacctgaa
     1201 ttccatatcg cagtttctca taatcactca tgattctgaa atctttgaga actctaatgt
     1261 ggacatgata tacaatttcg agtctactcc tgagggaaca aaagtaacta ctctctagcc
     1321 gcctgtaagt ttggcaaatc tttcattttt gcttagcttt tccataaagt gcaagtttga
     1381 gagtgcaaca actgctgatt cccttactgg ttcgctagag tctgtgagtg ccttttgcaa
     1441 agtctctttt gcctcctggg atcctatgac accaagcgct attgcagctt cgtgtcttac
     1501 aaagaagctt ggatcatttc ttaccgcatc ttcaagtggt tttattccgc ttctaaagca
     1561 catctgacct agtgaaaatg cagcctcgtg tcttacaagc tcatttgaat cattttttaa
     1621 aaccttggaa acatgggaaa tcatttcctc gcctccaaaa tcaactagaa tgcagactgc
     1681 tcttgttctg attacataat ctttgtgatc taacagctgg acaaaatact ccttgtcttt
     1741 tttctcatat ctttcttcca tctctgaaag aagctcaagt cgtgaatttg gaataacttc
     1801 catgcacaca attttataat cgaatattta ttcattggtc aaatgtcacg ctgtaggtac
     1861 tggcacctta ccatagaaga gctgaaaaaa gtaaactatg atcaaaaaaa ggttctagtc
     1921 tgggaaataa aatgttccaa agacgatagt ggaacacact ttggtgtatt ctgttacaga
     1981 aatggcactc cttgggatta caagtctgtt caaggcatag tgttttacta taacatgatt
     2041 ccgcttgatg aagtaaaaaa aataactgaa ttcctaaagg gaaagtttgg gggagaagaa
     2101 aaagaaaagg gagaacgtgt ttttcttgtg gattctcgtg tgatttactc tccaatagaa
     2161 atagctgatc ttgcaacaga gattggcaac aagtttgaga caagcgtcga gatttcagta
     2221 gagcttgaga actttagccc acaagagcaa gaacaaagca attttccatc aagcaaactt
     2281 ttaccaattc cgggcaagta gataaatata gttttggcgg tcttttagat ctcagatgga
     2341 cgagataaaa caaataggac cgcatcttga ggaactaaga aaacgattaa ttcgaatggt
     2401 tttagctgta gtgataatta ctgcatttgt tttgactttt catctttatg cagttgatta
     2461 caatggcatg acactttact atccacttcc tgatccgctc aacaacattg cagctcaact
     2521 tactttggaa atgaaaaatc aacttgttcc agaaactgtt cagctgatac aaactgctcc
     2581 gggtcaagcc ttctttgcac agttttatgt tgcagcgctg cttggcattg tttttggaat
     2641 gcctgtgata gtaaaggagc ttgtaagttt tgtatctcct gcattgggaa aaaaagagac
     2701 tagaatcatt agatcaatga caattccagc aataggactt ttcataactg ggtgtgtgtt
     2761 ttcatatttc tttgtcacac catacatttt ggaatttctt tatacttatg gtgaggcaag
     2821 cggccttgtc acatttctta atgttgtaga ttttatcaca ttcgtactcc agtttctgtt
     2881 ggcttttgga atatcattcc aattgcctat tgtaatgtat gcagtttctg catcaggcct
     2941 agcagatgaa aagttttgga gaaaaaacat cagatatgca atagtaataa ttgtaatttt
     3001 tggagcaata attactcctg atggaagtgg aataacaatg tggtttgttg cagggcctat
     3061 gattggtctg tatcttttag gcatgatttt tactgaacga aaaggcagac agattgcaac
     3121 acttaaatct taatctgcgg aaattaaatt gatcatgcca gtagcaggta ccgaatggat
     3181 ttggatcata gttgcaatag gcgtcttaat ttttggtgca aaaaagattc cagaacttgc
     3241 acgcactttt ggtaaagcaa aaggcgaata tgaaaaagga aagatagaag cagacaagga
     3301 actaaaagaa ttcaaagaaa aaaacgaccc gaaataaaac aatacttttt tacccatact
     3361 caacaaatta gcttgtgata agacgttctg aaatccaaga aattgtcagt ggatattctg
     3421 atcttacagt aggaacactt gggagccact ctgcacttga aataatggac ggtgcaaagg
     3481 atgaaaattt gcctactctg gttgtctgcc aaagaggcag ggaaacacca tacaaaagat
     3541 tctcaaggat tgcagatgaa attgttctag tcaacaaatt tgcagaaatg ctttctccaa
     3601 agatgcagtc caagctgcga gcttcaaaca ctgtaattat acctcacagg gctcttactg
     3661 catatcttgg ttacaaaaga atagaaaata atttcaaggt accgatattt ggaaatcgcg
     3721 ccttgtttca ggcagaagaa aggtcgcaca gaaaaaacca gtactatctt cttgagaaag
     3781 caaggataaa gcacccaaaa cttttcaaaa acccaaaaga cattgacagg ccagcaatag
     3841 taaaggtaca ggaaaaatca agaaagcttg agagggcatt tttcaatgtt tcatcatttt
     3901 ctgattataa agacaaaact gggacaaaga taaggcacgg gttgatatca aaagaggacc
     3961 taaagattgc aagcattgag gagctggtga ttggtacata ctttaatttc aactattttc
     4021 acactccgat ttctgacgat gttgattttc ttggcattga aagaagactg cagacaaacc
     4081 tgcatga
//
LOCUS       DATIDL010000028        13563 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrosopumilaceae archaeon isolate SMAG_U16342
            k141_243603, whole genome shotgun sequence.
ACCESSION   DATIDL010000028 DATIDL010000000
VERSION     DATIDL010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935836
            Sequence Read Archive: SRR25158326
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosopumilaceae archaeon (soil metagenome)
  ORGANISM  Nitrosopumilaceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrosopumilales;
            Nitrosopumilaceae.
REFERENCE   1  (bases 1 to 13563)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13563)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/04/2024 05:09:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,520
            CDSs (total)                 :: 1,474
            Genes (coding)               :: 1,474
            CDSs (with protein)          :: 1,474
            Genes (RNA)                  :: 46
            rRNAs                        :: 1 (5S)
            complete rRNAs               :: 1 (5S)
            tRNAs                        :: 43
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13563
                     /organism="Nitrosopumilaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_243603"
                     /isolate="SMAG_U16342"
                     /isolation_source="soil"
                     /db_xref="taxon:2202732"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1600)
                     /locus_tag="VLB45_01205"
     CDS             complement(<1..1600)
                     /locus_tag="VLB45_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HSD04355.1"
                     /translation="MQANNLIKETSPYLLQHAYNPVNWYTWGGEALKKAKDENKPIFL
                     SIGYSACHWCHVMAHESFENEDVAQIMNENFINIKVDREERPDVDDIYQKVCQLVTGS
                     GGWPLSVFLTPDQKPFYVGTYFPILDSYGRPGFGSILRQLAIAYKEKPSDIEAAATNF
                     MNTLTKTEPINTRSKLEKSILDEAAINLLQIGDPVNGGFGQAPKFPNAANLSFMLRYS
                     KLSGISKFQEFVFKTLTKMANGGIYDQIGGGFHRYSTDSRWLVPHFEKMLYDNALLSI
                     VYAEAYQVAKDPKYLQVVNQTLQYVLREMTSPDGGFYSAQDADSEGEEGKFYVWKKQE
                     IQEILGDDSDVFCLYYDVTDGGNFEGHTILNNSVRTSTVAFQFGKTESEVEEIITRCS
                     KNLFEIRSKRIAPGRDDKILTSWNGLMISAFAKGYRISGLNEFLDAAQNCITFIEEKL
                     TKNGKLLRTYKNGQAKLMAYLDDYVYFVNALLDVFEINPDAKYLDLAVKHADYLIDHF
                     WDESENNFFFTADDHEKLIIRTKNL"
     gene            1672..1824
                     /locus_tag="VLB45_01210"
     CDS             1672..1824
                     /locus_tag="VLB45_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016939289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04356.1"
                     /translation="MNCKRCHHTDQAHEPSKNSTSLARVGKCLIPHCTCKQYLDPIEE
                     IDEDLL"
     gene            complement(1821..2069)
                     /locus_tag="VLB45_01215"
     CDS             complement(1821..2069)
                     /locus_tag="VLB45_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04357.1"
                     /translation="MKDPHNYRAVGWSMVAVAASLAIIGLLVLAIGDDAYFSDKIMKQ
                     KQAEFEAKKKLEQTEKSIGLDKNPSMASDSGLFILPVV"
     gene            complement(2241..2468)
                     /locus_tag="VLB45_01220"
     CDS             complement(2241..2468)
                     /locus_tag="VLB45_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04358.1"
                     /translation="MSKLDSKTHKTKKDMDALLSRLNALEASATDNFQKSIIGVLRAI
                     VETQSHSLDEFEHLKKALDLITLQLFKLDSK"
     gene            complement(2592..3365)
                     /locus_tag="VLB45_01225"
     CDS             complement(2592..3365)
                     /locus_tag="VLB45_01225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04359.1"
                     /translation="MERIVLGVSLFLILSSSVIIGNAHAEYVVLEDSKKQYSINIKFL
                     KYENKRKYYSGDDLILYSFRDDKFLRLIGKIDGVPMHLFGKKIFDGNDIDTYLLKGKT
                     ENGKKLQFTMKINDTKIGEVQVQPVVPTVQKLTNKINLVSFSPSVVTKGYSYNFFVRT
                     YDSQLNPIGNTEQNWGILQDVNVSIKILDKDSKVIKQFNGVTGELGYYNDGFVIPYDL
                     TASSYFVIINATKDGYSSDSDQFWLHILELITEGNTSNN"
     gene            3500..3955
                     /locus_tag="VLB45_01230"
     CDS             3500..3955
                     /locus_tag="VLB45_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HSD04360.1"
                     /translation="MSEEKLKMLGITLPNPPKPAGSYVPIVRTGKLVFVSGQIPMQDG
                     KIAYAGKVSKDLNLEEAQKAAKLCIINALAQIKSELGSLDKISKIVKVSGFVNSPPEF
                     NEHPKVINAASDLLFEIFGSKGQHTRIAVGVSSLPLNSAVEIDLIAEIN"
     gene            complement(3948..4928)
                     /locus_tag="VLB45_01235"
     CDS             complement(3948..4928)
                     /locus_tag="VLB45_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016939277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HSD04361.1"
                     /translation="MTRNKIVCVSHKEDADGIGAASLIRQAFGGETRLVDYPGLMNEL
                     ELLKNNESLKTLYICDLGLSKTNQDQFIELLKVLRKKKVSITYIDHHDLDEKLKKKIR
                     ALGVKLIHKIDECTTVQVYGAFKSKLTDHSAFLAACSAITDYMEDRPLGSKLLQKFDR
                     QFTLLEATVLTFTITSHQKDNEYLLYLVDELSESKYPHDIPNSFEFARIQAERISGVI
                     QKVKQNMKKMKNLAFMEVTDSGASMVVNFVLGMSGKDVGVSYKLREDHGIYAVSVRGS
                     RSLKTHLGRIVNPLATELGGSGGGHDKACGAVIPKDKIKIFLKKFNSKLN"
     gene            complement(4972..5193)
                     /locus_tag="VLB45_01240"
     CDS             complement(4972..5193)
                     /locus_tag="VLB45_01240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04362.1"
                     /translation="MTELERDFIKLVDDFVINSKEPEIIAEIGQLDREAQLLGITFYD
                     MYCIVLQNVAGHQNLISRFQIYMKTKKTV"
     gene            5340..6503
                     /locus_tag="VLB45_01245"
     CDS             5340..6503
                     /locus_tag="VLB45_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008299523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HSD04363.1"
                     /translation="METKNIGLRNIEVADTKISAIDGINGKLIYRGYDILDLVKKSTF
                     EETACMLLNDDLPTQNMLTDFFKELVSEREIPEGIEKNLKNFPKTSNPMDVLQSTVSS
                     LATYATDKSEDRQTNYRRAISLIAKLPTIISYWDRIRNDKELIRPSKKLNHAGNFLYM
                     LTGKEPDQEFARIFDICLILHAEHSFNASTFAAREVSSTRAHMYAAVSAAIGALSGEL
                     HGGANFQVMQMLLDIKSESKVESWVKEKLGQGKKIMGMGHAVYKTFDPRAEVLRELSR
                     RLSEKTGEPWFKITKKVEDVTAEEMKKVKGSDIFPNVDLYSASTYYMLKIPPDLNTPI
                     FAVSRVAGWTAHIIEERFAEAAPKPMLYRPKAVYVGKYCGPSGCEYVPLEKRE"
     gene            complement(6504..6710)
                     /locus_tag="VLB45_01250"
     CDS             complement(6504..6710)
                     /locus_tag="VLB45_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008299161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04364.1"
                     /translation="MLEPHSFFTNMVDELIEFSEYDPELKDGLKWIDSEAQKRGITFY
                     EMVFVVLHKYDADVKAKEWLQTRN"
     gene            6788..7174
                     /locus_tag="VLB45_01255"
     CDS             6788..7174
                     /locus_tag="VLB45_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010190411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04365.1"
                     /translation="MDHVYFSDGTEKRISWVIQNNETQVEQYREQTDIYLDKVSIEQA
                     KYIALHVGIFWGVGRFIIKNDDSVKVMVDSRSMFDRLVDKKTSSDPFIESRIGFIRQI
                     IDQRKLVVKYELIEPQQNIATKVLSS"
     gene            complement(7163..8389)
                     /locus_tag="VLB45_01260"
     CDS             complement(7163..8389)
                     /locus_tag="VLB45_01260"
                     /inference="COORDINATES: protein motif:HMM:NF025042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4147 domain-containing protein"
                     /protein_id="HSD04366.1"
                     /translation="MQILEKGLDTALPNPFLSRVVHKNTLFIGKNRISLSKYDRIFVV
                     AFGKAADSMTSVIGRLIDIKGGIVVIPKNVDSVLKNKNFKIIRAGHPIPDKDSVFSAR
                     MIIEFLKTRKKDDFVFFLISGGGSSLIALPDGVTLSDKKKITKLLLDCGANIHEINCI
                     RKHISKIKGGWMSKYLKCDAVSLVMSDVVGNDLSSIASGPTYFDKTTFDYAAKVLKKH
                     GLEKSVPRSVLKRIKLGIEHKIPETPKRITIKNYIIATNEDCLDEMKKKAVSFGLRTK
                     IIHSMAGDVRAIAHKLVSASKSYDCLIFGGETTVKVRGNGKGGRNQELVLHALMESYK
                     KNQNMIIASIGTDGIDGNTDACGAILSTGELKINQTQKFLKNNNAYSLLKKQNALIFT
                     GPTHTNMMDIGVVLRR"
     gene            8511..9455
                     /locus_tag="VLB45_01265"
     CDS             8511..9455
                     /locus_tag="VLB45_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007550926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HSD04367.1"
                     /translation="MNTIRIPKIIKFGKNALSETEYPKNALVVTTAPPDVSSKWLAKM
                     GIKDYMLFDKVEPEPSIETVQKVISDYKSKNLSAIIGLGGGSSQDVAKYAASEIKIPK
                     ILIPTTFGTGSEMTTYCVLKFDGKKKLLQEEKFLADMAVVDSYFLDGTPEAIIKNSVC
                     DACAQATEGYDSKRGNEFTRTMCKAAFDVLYDAIMNNKPENYPYGSMLSGIGFGNAST
                     TLGHALSYVFSNEGVPHGYSLSSCTTVAHRFNKSIFYEKFKEVIEKLKFDRLPLKAPI
                     DQAADVVMTDKGHLDPNPIPVTKEDVMQLLQDIKDGRL"
     gene            9567..10652
                     /locus_tag="VLB45_01270"
     CDS             9567..10652
                     /locus_tag="VLB45_01270"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016939947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HSD04368.1"
                     /translation="MIKFGIQQGLNVARLGLSEDQILTACILADRTGYNSIWYMDHTN
                     VPQWNHAIVNDPWVMLSAIASVTNNVELGTCVTDAVRRHPSNIALATVTLDRVSHGRG
                     TLGIGAGEAQNIKEFKIQWDKPVGRFEEQLQIIKLLFESSPEHRVSFKGEFYQLEEAC
                     LQAKSVRQPHPPIYMAAGAPRTLGLCGKYGDGWIPIGYTPELYQVHANVIKESIKENG
                     RDKNDFQFGVDIDVYFSDDAEEAWAKMKNAVKVSLFKPEVLKVHNIKDIEGFDFKKYF
                     TEYSMSNQEWIVKMREAAQTIPDKVARSSIALGTPDDIIPVFEKFLKAGVNHFIIRFW
                     GSGYFGSIDKFGSTVLPYFREMDNPKK"
     gene            10690..11592
                     /locus_tag="VLB45_01275"
     CDS             10690..11592
                     /locus_tag="VLB45_01275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04369.1"
                     /translation="MNLFLLAFVTVFTISAYGSQNTISDANIDVSLNYPDVVAPGNEF
                     VLASTVKTKADQMSNITVTIGSPELEIVQNQFNIPSLPRESTLGNNFNAKIKPDSPDG
                     IVVANISIEYFIKGFFDEKPIKNSLIKAFEINVQSKPIILINIDSPDSVFSGEQFSIK
                     GTIKNQGYDAQNIEIIADSPQVMLDGKKIYAITNLDAGRIQNFEFVLQTPKDLAIPTD
                     ITVNISTTYSDKSGKTYSQEDALNIFTRQRGILEIGGAEGLWVGDFFIAPVVGVGTIV
                     SSVIGFFIFLWHIKNKKKQKRTKK"
     gene            complement(11593..12003)
                     /locus_tag="VLB45_01280"
     CDS             complement(11593..12003)
                     /locus_tag="VLB45_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04370.1"
                     /translation="MKIKEQFTDFKAFLLAGSAVMAFSHFVYNLFGYTIPAIVWEFFK
                     EFGAAIIMMSVFVFAIAWLLKAKPHKKPQNYSVISYDVFGNESKIDGMRTEFKTHDVA
                     WSFMKHYKKSYPLYNFALVSDLPNSEKKTIFKYL"
     gene            12095..12703
                     /locus_tag="VLB45_01285"
     CDS             12095..12703
                     /locus_tag="VLB45_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HSD04371.1"
                     /translation="MKSKEKIILFLNQQETGRICSVDKDGYPQIIPMNFVYTNDAIYM
                     HSHTKGEKLENIKKNSKVGFEVDQSLEFLPSYFTDPKDASQADTLYISVVIKGIGTIV
                     ENRNEKTMALNALMKKYQPEGGYESISPEMRVVDEVAIIKIIPSTIRGKYKVGQHLDK
                     KTRVELARKIIERNSPTAQNTLKMMGIIQTKDGFEITEEPSW"
     gene            12752..>13563
                     /locus_tag="VLB45_01290"
     CDS             12752..>13563
                     /locus_tag="VLB45_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008299372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HSD04372.1"
                     /translation="MLLQNQCFELSAEFQPTGDQPNAIELLVNEVQKKKIQTLLGVTG
                     SGKTFTIANVIQRTGKNTLVISHNKTLAAQLYSELKQFFPKNNVGYFVSYYDYYQPES
                     YIPQTDTYIEKDTQINEKIEKMRLEATAMLLSGEPTIIVATVSCIYSLGSPRDWEAMS
                     ISIENGATVSRSALIKQLVNARYERNDTELSSGRFRIKGDTLDIIPAYSDDVIRISMF
                     GDKIEKISVHDNISLKEKKTVSKIKIFPAKHYLVANDVRQAAVNSIKKELEQ"
BASE COUNT         4547 a         2434 c         2354 g         4228 t
ORIGIN      
        1 agagattttt tgttctaatg attagttttt catgatcatc agcagtaaaa aagaaattgt
       61 tttcagattc atcccaaaag tggtctatta aataatctgc atgttttaca gcaagatcta
      121 gatactttgc atcaggattt atctcaaaga catccaataa tgcatttaca aaatacacat
      181 agtcatcaag atatgccatt agtttagctt gaccgttttt gtacgttcgg agaagtttac
      241 cattttttgt tagcttttct tctataaacg taatacagtt ttgtgcagca tcaagaaact
      301 cattcaagcc gcttatccta tatcctttgg caaatgcgga aatcattagt ccattccaac
      361 tggtcagaat cttgtcatct cttcccggtg caattctttt agagcgaatc tcaaagagat
      421 tttttgaaca cctagtaatt atttcctcta cctcagactc agtttttcca aattgaaatg
      481 ctacggttga tgttctaaca ctattgttaa gtatcgtgtg gccttcaaaa ttaccaccat
      541 cggtgacatc ataataaaga caaaaaacat cagaatcgtc tcccagaatt tcttgtattt
      601 cttgtttttt ccagacatag aattttcctt cttctccttc tgaatcagca tcttgtgcag
      661 aataaaatcc tccatcagga gaagtcatct cacgtaaaac atattgtagg gtttgattca
      721 caacctgaag atattttgga tcctttgcta cttgatacgc ttcggcatac actattgata
      781 gcaatgcatt atcatacaac atcttttcaa aatgcggtac aagccatcta gaatcggtag
      841 aatatctgtg gaatcctcca ccaatctgat catatattcc tccatttgcc atttttgtca
      901 aggttttaaa aacaaattct tggaatttcg atattcctga aagtttagaa taacgtagca
      961 tgaaagaaag atttgcagca tttggaaatt ttggtgcttg cccaaatccc ccatttacgg
     1021 gatcaccaat ctgaagcaaa ttaattgcag cttcatctaa aattgatttt tcaagtttag
     1081 atcgagtgtt aattggctct gtttttgtaa gagtattcat aaaattagtt gctgcagctt
     1141 caatgtcaga tggtttttct ttgtatgcaa ttgcaagctg tcgcaatata cttccaaacc
     1201 caggtcttcc ataactatca agaattggga aatatgttcc aacatagaat ggtttttggt
     1261 caggagtaag aaatacactg agtggccatc ctccgcttcc agtaactaac tgacaaactt
     1321 tttggtaaat gtcatcaaca tctggccgtt cttccctgtc tacctttatg ttgatgaaat
     1381 tttcattcat aatctgtgca acatcttcat tctcaaacga ctcgtgcgcc ataacatgac
     1441 accaatggca cgcgctgtac cctatgctta gaaatattgg tttgttctca tcttttgctt
     1501 tttttagtgc ttctcctccc caagtgtacc aatttactgg attgtacgca tgttgtaaga
     1561 gatatgggct ggtttctttt atgaggttgt ttgcctgcat gatctagttt cttgatgtgt
     1621 ctatttgtat gtaattcaag tattaaataa aacaaacaat ttcttttaaa catgaattgc
     1681 aagcgatgcc atcatacaga tcaggctcat gaaccctcaa aaaacagtac atcgcttgca
     1741 agagttggca aatgtcttat tccacactgc acatgtaaac aatatctaga cccaattgaa
     1801 gaaattgacg aagacttact ttagacaaca ggtaaaataa acaatccaga atctgatgcc
     1861 atacttgggt ttttatctag cccaatagat ttttcggttt gctctagttt cttttttgct
     1921 tcaaattcag cttgtttttg tttcatgatt ttatcagaaa aatatgcgtc gtctcctatt
     1981 gccaatacta aaagaccaat aattgcaagc gaagcagcaa ctgccaccat agaccaacct
     2041 actgctctat aattatgagg atctttcatt tgtttcaaag caaagaaacc ctgaatttaa
     2101 tcttttattc atgttgatat cataacacat catgcaatgt ccatgttgtg gatcaaaaat
     2161 ggaaaaagtg caagagcttg ataatagtta tcttataaaa tgcgtagaat gtggtctaag
     2221 caatacggta ccaaaatgat ctattttgaa tctaatttga aaagctgcaa agtaatcaag
     2281 tctagtgctt ttttcaagtg ctcaaactca tctagcgaat gagattgtgt ctctacaatt
     2341 gctcttagta ctccaattat tgatttttga aagttatcag tagcagacgc ttcaagtgca
     2401 tttagtcttg aaagtagtgc atccatatct tttttggtct tgtgagtttt ggagtctagc
     2461 ttcgacatat aatttgtttt tttatacaat atatgaagat agagtcatat tttgtttatc
     2521 tttgttttag gaacagtaag caattattgg ctaattttgt tagttggtct gaactatagg
     2581 agattgtgtc atcaattatt cgatgtgttt ccttctgtaa ttagttccaa tatgtgaagc
     2641 cagaactgat cactgtcgga agaatatcca tcttttgtag catttatgat cacaaaatag
     2701 gaagaagctg tgagatcata tggtatcaca aaaccatcat tgtaataacc cagttcaccg
     2761 gtaacaccat taaactgttt tatcacctta ctatccttgt ctaatatctt aattgaaacg
     2821 tttacatctt gtaagattcc ccagttttgt tcagtattgc caataggatt taattgagaa
     2881 tcatatgttc gtacaaaaaa attatatgaa tatccttttg tcactactga agggctgaaa
     2941 gaaacaaggt ttattttatt tgttaatttc tgcaccgtag gtactacagg ttgtacttgt
     3001 acctctccga ttttggtatc atttattttc attgtgaatt gtaatttttt accattttcg
     3061 gttttaccct ttaacaagta tgtatcaata tcattaccat caaaaatttt cttcccaaag
     3121 agatgcatag gcacgccgtc aatttttcct attaatctaa gaaacttatc gtcacgaaaa
     3181 gaatagagta tcagatcatc accagaataa tattttctct tattttcgta tttgaggaac
     3241 ttaatattta tcgaatactg tttttttgaa tcttcaagta ctacatattc agcatgtgca
     3301 ttgccaataa tgaccgatga actgagaatt aagaagagtg agacacccag aactattctt
     3361 tccattgtaa tacaatgatg tgatttcgta ttaactttat aggcaaaata ttgagagtga
     3421 atgtattgtt gagaacagtt attcagtttt atctatttgt gaagataaag ttaaaatgga
     3481 tacagtaaaa gcagattttg tgagcgaaga aaaactaaag atgcttggta taacgttacc
     3541 aaatcctcca aagcctgcag gatcttacgt cccaatagtc agaacaggaa aactagtatt
     3601 tgtttcaggt cagattccaa tgcaagacgg caagatagca tatgcaggaa aggtttcaaa
     3661 ggatcttaat cttgaagaag ctcaaaaagc agccaaactg tgcatcataa acgcactagc
     3721 ccagataaaa tcagagcttg gaagcttgga taaaatttca aagattgtca aagtatctgg
     3781 ttttgtaaat tcgccaccag aattcaatga acatccaaag gtcatcaatg ctgcgtcaga
     3841 tttacttttt gagatttttg ggtcaaaggg tcaacatacg cgtattgcag taggagtctc
     3901 aagcttacca ctaaattctg cagttgaaat tgatcttatc gcagagatca attaagtttt
     3961 gagttaaatt ttttcaaaaa aatctttatc ttgtcttttg gaattactgc tccacatgct
     4021 ttatcatgtc ctccaccaga accaccaagc tccgttgcaa gaggattaac tattctacca
     4081 agatgggttt tcaagcttct tgaaccacgt actgagacag cgtatattcc atgatcttct
     4141 ctgagtttgt atgaaacacc aacatccttg ccagacattc ctaaaacaaa attaaccacc
     4201 atacttgcgc cagaatctgt aacttccata aatgcaaggt ttttcatttt tttcatgttt
     4261 tgttttactt tttgtattac tccagatatt cgctctgcct gtattctagc aaactcaaat
     4321 gagttaggaa tatcatgtgg atattttgat tcgcttagct catctacaag ataaagaaga
     4381 tattcattgt ctttttgatg gcttgttatt gtaaaagtaa gaactgtcgc ctcaagcaat
     4441 gtaaactgtc tgtcaaattt ctgtagcaat ttagaaccaa gtggtctatc ttccatatag
     4501 tcagttatag cagaacatgc agccaaaaat gcgctatgat ctgtaagttt tgatttaaat
     4561 gcgccatata cctgaacggt agtacattca tcaattttat gaatcagttt tactccgagt
     4621 gcacgaattt tctttttgag tttttcatcc aaatcatgat gatcgatgta tgtaattgag
     4681 accttcttct ttcttaatac ttttaataat tcaataaatt gatcttgatt tgtcttgctt
     4741 agtcctaaat cacaaatgta aagtgtcttt aatgactcat tattttttaa gagttcaagc
     4801 tcattcatca agccaggata atctacaagt cgtgtttctc caccaaatgc ttgtcgaatt
     4861 aacgaagctg cgcctattcc atctgcatct tccttgtgag acacacaaac aattttgttt
     4921 cttgtcatga atagtcaaga cacattcacc ctcatttaaa ctaaaaaaca atcaaacggt
     4981 tttctttgtt ttcatgtaaa tttggaatct agaaataaga ttctgatgac cagcaacatt
     5041 ttgtaaaact atacaataca tatcataaaa tgtaattccc aacaattgtg cttctctgtc
     5101 aagctgccca atctctgcta taatttctgg ttcttttgaa ttaattacaa aatcatctac
     5161 tagtttgata aaatctcgct ctaattcagt catttctact ttaaccagaa aatttatgct
     5221 atatggaaaa gagttgccag attagacaaa ttgcttaatt ttttaattaa ttttcataca
     5281 tatcacaaaa tgtttgtttc gtatagcttt aatatgcaat tttggaaatt actagtgtag
     5341 tggagacaaa aaacataggt cttcgtaata tcgaggtagc tgatacaaag atttctgcaa
     5401 tagatggaat aaacggtaaa ctgatctata gaggatatga cattttagat cttgtcaaaa
     5461 aatccacttt tgaagaaacc gcttgtatgt tactcaacga tgacttgccc acgcaaaata
     5521 tgctcactga tttcttcaag gagttagtat cagaacgtga gattccagaa ggaattgaaa
     5581 aaaatctcaa aaactttcct aagacatcaa atccaatgga tgtcttgcag tcaacagtct
     5641 cctcacttgc tacctatgca acagacaaat ctgaagacag acagacaaac tatcgtagag
     5701 caatcagtct aattgcaaaa ttacccacaa tcatatctta ttgggataga attcgcaatg
     5761 ataaggaact aattagacca tcaaagaagc taaaccacgc aggcaacttt ctttacatgt
     5821 taactgggaa agagccggac caagaatttg caagaatttt cgatatttgt ttaattttac
     5881 atgcagaaca cagttttaat gcatcaactt ttgccgcaag ggaggtttca tcaacaagag
     5941 cacacatgta tgctgcagta agcgctgcaa ttggagcatt aagtggagaa ctacacggtg
     6001 gagcaaattt ccaagttatg cagatgcttt tagacataaa atctgaaagt aaagttgaat
     6061 catgggttaa agaaaaactt ggccaaggta aaaaaataat gggcatgggc catgcagtat
     6121 acaagacgtt tgatccacgc gcagaggtat taagagagct ctcaagacga ttatcagaaa
     6181 aaacaggtga accgtggttt aagattacaa aaaaagtaga agatgttact gcagaggaga
     6241 tgaaaaaagt aaaaggctcc gacatatttc caaatgtaga tctttacagc gcttcaacat
     6301 actacatgct aaaaattcca ccagatctga atacgccaat atttgcagtt tccagagttg
     6361 caggatggac tgcgcatatt attgaagaaa gatttgcaga ggcagcacca aaacccatgc
     6421 tttacagacc aaaggcagtg tatgttggca aatattgtgg tccgtctgga tgcgaatacg
     6481 taccgttaga aaaaagagaa tagctagttt cttgtctgaa gccactcttt agctttgaca
     6541 tctgcatcgt atttgtgcaa taccacaaac accatctcat agaatgttat tccacgtttt
     6601 tgtgcctcac tgtcaatcca tttgagccca tcttttagct ctggatcata ttcagaaaac
     6661 tctatcagct catccaccat gttggtgaaa aaggagtgag gctccagcat tgctacctgg
     6721 tttctatttt ggttattcag ccctgtattc aaaatatata gactatcgac acgtttttgg
     6781 ttgacatatg gatcatgtct atttttcaga tgggactgaa aaaagaatct catgggtcat
     6841 tcaaaataat gaaactcagg tagagcagta tagagaacag acagacatct acctggataa
     6901 ggtatcaata gaacaggcaa aatacattgc acttcatgtt ggaatattct ggggcgttgg
     6961 tagatttatt ataaaaaatg atgacagcgt aaaggtaatg gtggattcga gatcaatgtt
     7021 tgatcgtctt gttgataaaa aaactagctc agatccattc atagaatcaa gaattggctt
     7081 cataagacag ataatagatc aaagaaaact agttgttaaa tacgaactaa tagaaccaca
     7141 acaaaacatt gcaacaaaag ttctatcgtc gtagtactac accaatatcc atcatattag
     7201 tatgggttgg gcctgtaaat atgagcgcat tttgtttttt taataaggaa taggcattat
     7261 tatttttcaa gaatttctga gtctggttta tttttaattc tccagtactc agtattgcac
     7321 cacatgcatc tgtatttcca tcaataccat cagtcccaat tgatgcaatt atcatgtttt
     7381 gatttttttt gtaagattcc atcaaggcat gcaaaactag ttcttgattt ctaccaccct
     7441 tgccattacc tcgtaccttt acggtggttt ccccgccaaa gataagacag tcataggatt
     7501 ttgatgcact gacaagtttg tgagcaattg ccctcacatc acctgccatt gaatggataa
     7561 tttttgtgcg caagccaaaa gacacggctt tttttttcat ttcatctaga cagtcttcat
     7621 ttgtagcaat tatgtaattt tttattgtga tacgttttgg ggtttcggga attttgtgtt
     7681 caattccaag tttgatcctt ttcaatacac ttcgtggtac ggatttttca agtccatgtt
     7741 tttttaacac ttttgcggca taatcaaacg tagttttatc gaaatatgtt ggtccagatg
     7801 ctatagaaga aagatcattt ccaacgacat ctgacattac aagagaaaca gcatcacatt
     7861 tcagatattt tgacatccat cctcctttta tttttgagat gtgctttctg atacagttga
     7921 tctcatgtat atttgcacca caatcaagta agagctttgt aattttcttt ttatcagata
     7981 atgtaacacc atctggaagt gcaatcagtg atgaaccacc tccagatatt aaaaaaaaca
     8041 caaaatcatc tttttttctt gtttttaaaa attctataat cattcttgca gaaaaaacac
     8101 tgtctttatc tggaatagga tgcccggcgc gtataatttt aaaattttta tttttaagta
     8161 cagaatctac attttttgga attacaacta ttcctccttt gatatcaata aggcgcccaa
     8221 tcacacttgt catcgaatca gccgcttttc caaaggcaac tacaaaaatt ctatcatatt
     8281 ttgacagcga gattctgttt ttgcctatga aaagtgtgtt tttgtgcacg actctgctta
     8341 aaaatgggtt gggaagagcc gtatctagtc ctttttctaa tatttgcaat gcaattctct
     8401 tatctctgtt tgatcccaag gattgatagt ttttaattat caagtacgat gctcatccaa
     8461 cctagttgtg tctgtaaggt ataataataa tcacaaatct aactttgagc atgaatacaa
     8521 ttagaatacc aaaaataatc aaattcggca aaaatgcact ttctgaaaca gaatatccaa
     8581 aaaatgcact agttgtaaca actgcaccgc cggatgtttc ttcaaagtgg cttgcaaaga
     8641 tgggcattaa agattacatg ttatttgata aggtagaacc agaaccatct atagaaacag
     8701 tgcagaaagt aatttccgat tacaagtcaa aaaatttatc agctataatt ggactgggcg
     8761 gaggaagttc acaggatgta gcaaaatatg cagcatcaga gataaaaatt ccaaagatct
     8821 taatccctac aacttttgga acaggaagtg agatgactac atactgcgtg ctaaaatttg
     8881 atggcaaaaa gaagctacta caagaagaga aatttcttgc agacatggca gtagttgatt
     8941 catacttttt agatggtacc ccagaagcaa ttattaaaaa ttcagtatgt gacgcatgtg
     9001 cacaagcaac tgagggatat gacagtaaaa gaggaaacga gtttacaaga acaatgtgta
     9061 aagctgcatt tgatgtacta tatgatgcaa taatgaacaa caaacctgaa aactatccat
     9121 acggttcaat gctttctggc attggttttg gaaatgcctc aacgactctt ggacatgctc
     9181 tttcgtatgt attttcaaat gaaggggtac cccatggata ttcactttct tcttgtacta
     9241 cagttgccca taggtttaac aaatcaatat tttatgaaaa attcaaagag gttattgaaa
     9301 aactaaagtt tgacagatta cctttaaagg cacctattga tcaggcagca gacgttgtga
     9361 tgactgataa aggacacttg gatccaaatc caattcccgt tacaaaagaa gatgtcatgc
     9421 aacttctgca agatatcaaa gatggtagat tatagaaatc tacaactttt gtttatattt
     9481 tttaaataac gagttatgat attcgtacat tttactagac ttaaatactg ctttcaaatc
     9541 acaatgatgt tataggattg taaaaaatga tcaaatttgg aattcaacaa ggacttaatg
     9601 ttgcaaggct gggattgagc gaagatcaaa tcctcacagc ttgcattttg gcagacagga
     9661 caggatacaa ttcgatttgg tatatggacc acacaaatgt accacaatgg aatcatgcga
     9721 ttgttaatga tccttgggta atgctttctg caatagcatc tgttacaaat aatgttgagc
     9781 ttggaacatg cgttactgac gcagtgagaa gacatccatc aaacattgcc ttggcaacag
     9841 ttacgttaga tagagtatca catggccgag gaacacttgg tatcggcgca ggtgaggcac
     9901 aaaacattaa agagtttaag attcaatggg ataaacccgt tggcagattt gaagaacagc
     9961 ttcaaatcat aaagttactt tttgaatcat cacctgaaca tagggtcagt ttcaaaggtg
    10021 agttttatca attagaagag gcatgtctgc aagcaaaaag tgtgagacag ccacatccac
    10081 caatctacat ggcagctggt gccccaagaa ctttaggatt gtgtggcaag tatggggatg
    10141 gttggattcc aattggttat accccagaac tttatcaagt ccacgccaat gtcataaagg
    10201 agtcaataaa agaaaatgga agagacaaaa acgactttca atttggagtt gacatcgacg
    10261 tatacttttc tgatgatgct gaggaagctt gggcaaaaat gaaaaatgca gtaaaagtta
    10321 gtttattcaa accagaggtt ctcaaggtac acaatataaa agacatagaa ggatttgatt
    10381 tcaaaaaata ctttacagaa tattcaatgt caaatcaaga atggatagtc aagatgcgag
    10441 aagccgcaca aacaatacct gacaaggtag cacgctcatc aattgcactt ggaacaccag
    10501 atgacattat tccagtattt gagaaattcc taaaggcagg agtaaaccat tttataatca
    10561 gattttgggg atcaggatac tttggttcaa tagacaaatt tggttccaca gtattaccat
    10621 actttaggga aatggacaac ccaaaaaaat aattcaaaca atataagttt ggacttcgac
    10681 ctaaaatctg tgaatctctt tcttttagct tttgtcacag tatttacaat ttctgcatac
    10741 ggttcacaaa acacaatctc tgatgcaaat atcgatgtat cattgaatta tccagatgta
    10801 gtggcaccag gaaatgaatt cgttttggca tcaacggtta aaacaaaagc agatcaaatg
    10861 tcaaacatca ctgtaactat cggatctccc gagcttgaaa tagtacaaaa tcaattcaac
    10921 ataccaagtc tcccaagaga atctacactt ggaaataatt ttaatgccaa aataaaacca
    10981 gattcaccag atggaatagt ggtagcaaat atttcaatag agtatttcat aaagggattt
    11041 tttgatgaaa aaccaataaa aaattcactg ataaaggcat ttgagataaa cgtacaatca
    11101 aaacccataa ttttgatcaa tattgattct ccagactctg ttttttcagg agaacaattt
    11161 tcaattaagg gtacaataaa aaaccaaggg tatgatgcac aaaatatcga aattattgcg
    11221 gactcgcctc aagtaatgct tgatgggaaa aagatctacg caataactaa tctagatgca
    11281 ggaagaatac agaattttga gtttgtgctt cagacaccaa aagatcttgc aataccaact
    11341 gacattacag taaacatatc aaccacctat tcagacaaat caggaaaaac ttacagtcaa
    11401 gaagacgctt tgaatatatt tacaagacaa cgtggaatac tagaaatagg cggtgcagaa
    11461 ggactttggg ttggagactt tttcatagct ccagttgttg gggttggaac aattgtttca
    11521 tcagtcattg gatttttcat atttctttgg catataaaaa acaagaaaaa acaaaagcga
    11581 accaagaaat aactacagat atttgaaaat ggtctttttc tctgagttcg gaagatcgga
    11641 aactagagca aaattataca atgggtatga ttttttgtag tgtttcatga agctccacgc
    11701 aacatcatga gtcttaaatt ctgttcgcat cccgtcaatt tttgattcat ttccaaaaac
    11761 atcatatgaa atgacagaat agttttgagg ctttttgtgt ggttttgctt ttaataacca
    11821 tgctattgca aatacaaata cagacatcat aattatagct gcaccaaatt ctttgaaaaa
    11881 ctcccaaact atggcaggta ttgtatatcc aaataagtta tacacaaaat gtgaaaacgc
    11941 catcacggca cttcctgcaa gtaaaaatgc tttaaaatca gtaaattgtt ctttaatttt
    12001 catactagta ttaggataga cctgcttatt ttcttttaga tttatatcat ttaatctact
    12061 gactagcaat atgcaattac ttgggagatt ggagatgaaa tcaaaagaaa agatcatctt
    12121 gtttctaaat caacaagaaa ctggcaggat ttgtagtgta gataaggacg gatacccgca
    12181 aataattcca atgaattttg tctacacaaa tgatgcgatc tacatgcatt ctcatacaaa
    12241 aggcgaaaaa ctcgaaaata ttaaaaaaaa tagcaaagtc ggttttgaag tagaccaaag
    12301 tcttgaattt cttccgtctt attttacaga cccaaaagac gcttcacagg cagacacact
    12361 gtatatcagc gtagtcataa agggaattgg cacaatagtt gaaaacagaa atgaaaaaac
    12421 catggcccta aatgcgctca tgaaaaaata ccaaccagaa ggcggttatg aaagcataag
    12481 ccctgaaatg agagtagtag atgaggttgc aataataaaa ataataccaa gcaccatacg
    12541 tggaaaatac aaagttggtc agcatttaga taaaaaaacg cgtgtggagc ttgccagaaa
    12601 aataatagag aggaactcgc ctactgcaca aaataccctt aagatgatgg gtataataca
    12661 aacaaaagat gggtttgaaa taacagaaga accgtcctgg tagtaatact aatactacca
    12721 ttgctttaaa tttcgttttc agtctcttgt agtgttgtta caaaatcaat gctttgaact
    12781 gtcagcagaa tttcagccaa ctggagatca acccaatgca atagaattgt tagtcaacga
    12841 agtgcaaaag aaaaagatcc agaccctgct tggggtaact ggcagtggca agacgtttac
    12901 catagcaaac gtaatacaaa gaactggaaa aaacacactg gtaatatcac ataacaaaac
    12961 tttggctgct cagctttatt cagagctaaa acaatttttc ccaaaaaaca atgtagggta
    13021 ctttgttagc tattacgatt actatcaacc agaaagctat attccacaaa ctgatactta
    13081 catagaaaaa gatacacaga tcaatgaaaa aattgaaaaa atgcgtctgg aagcaacagc
    13141 catgcttcta tctggagaac caacaattat cgttgcaaca gtttcatgca tctattcact
    13201 tggttcacca cgtgattggg aagcaatgtc aattagtatt gaaaatggtg ccaccgttag
    13261 tcgctctgct ttgataaaac agctcgtcaa tgcacgttat gagagaaatg acaccgaact
    13321 ttcatcagga agatttcgaa taaaaggaga tacactagat atcatacctg cctattctga
    13381 tgatgtcata agaatctcca tgtttggaga caagatagaa aagatatccg tgcacgacaa
    13441 tatctcgctt aaagagaaaa agaccgtttc gaagatcaag atatttcctg caaaacacta
    13501 tcttgttgca aacgatgtta gacaagctgc agtaaactcg ataaaaaaag aattggagca
    13561 aag
//
LOCUS       DATIDL010000029         3018 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrosopumilaceae archaeon isolate SMAG_U16342
            k141_2262155, whole genome shotgun sequence.
ACCESSION   DATIDL010000029 DATIDL010000000
VERSION     DATIDL010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935836
            Sequence Read Archive: SRR25158326
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosopumilaceae archaeon (soil metagenome)
  ORGANISM  Nitrosopumilaceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrosopumilales;
            Nitrosopumilaceae.
REFERENCE   1  (bases 1 to 3018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/04/2024 05:09:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,520
            CDSs (total)                 :: 1,474
            Genes (coding)               :: 1,474
            CDSs (with protein)          :: 1,474
            Genes (RNA)                  :: 46
            rRNAs                        :: 1 (5S)
            complete rRNAs               :: 1 (5S)
            tRNAs                        :: 43
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3018
                     /organism="Nitrosopumilaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2262155"
                     /isolate="SMAG_U16342"
                     /isolation_source="soil"
                     /db_xref="taxon:2202732"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1425)
                     /locus_tag="VLB45_01295"
     CDS             complement(<1..1425)
                     /locus_tag="VLB45_01295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04373.1"
                     /translation="MKFFHILIIVTIAILIGIVFDAQTALGGGWYPCCIEFKLDKTEY
                     NTGDTVIITVRADQQYENSNENVIVKISDVTFGPDYANVIHQEEKTLEDGKAMFEYKT
                     PQSPDRYRYLLTLESPAGVQSKLFFTKKDASKFVISDIKVLNPVLKQGEPVKFEAKVV
                     DGVGNPAHYARILVTGDIPHETCSTEISGYAGAYLDLSPLYSLQPDYWSSGVVKGDVP
                     ITNTVMPGKYDLQVSVGGDIDGYVSSFEYFQIEIIPTDLPEDPPYTVFVPFQYDFGPG
                     FMTEKPINITAKTAYNGCGSIIPNVPIKAEIKRYDLQNAQWMETLQTKEITSDENGVY
                     HVSFEPIGLRAGYYNVLLTSTYQGVEQTIGVGTPNNIKNFTISEEGKDFTVTVDGWYF
                     VPLDVTFDKENKKLTLELDTFDPFRRVDFKIPHELLDGEFRTVLVNGKERDASAQKYE
                     GYTWFSPWPGEDDHTVIEVIGTSAI"
     gene            1920..2411
                     /locus_tag="VLB45_01300"
     CDS             1920..2411
                     /locus_tag="VLB45_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016940320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HSD04374.1"
                     /translation="MPLDIMDETDLRDIPTRERLNRCIYTIKNDADESKRWDAVYLAG
                     EIAEANPTDPIFDEVAELMSWILQNDDNGVIKHEVCFQIAARNMRKKIPDLINTALND
                     KSGIAKHEALECLALMKAFEAKDTMIKALKDPISHVRETAAFALKRLDRLDKKAYTPS
                     KIL"
     gene            complement(2654..2806)
                     /locus_tag="VLB45_01305"
     CDS             complement(2654..2806)
                     /locus_tag="VLB45_01305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04375.1"
                     /translation="MGGFEKNCPICNKKFTSLLEHTMHIGHDHKDILPGQIFESGKEK
                     KEHLRE"
BASE COUNT          873 a          594 c          563 g          988 t
ORIGIN      
        1 aatggcactg gttccaatta cttcaatgac agtatgatca tcttcgccag gccaaggaga
       61 aaaccatgtg tatccttcat atttttgggc agaagcatct ctttctttac catttaccag
      121 aactgttcta aattcgccat ctaacaattc gtgtggaatt ttaaaatcta ctcgcctaaa
      181 cggatcaaac gtgtcaagct ccaatgtgag ttttttgttt tctttatcaa atgtgacatc
      241 tagtgggaca aagtaccacc catcaactgt aactgtaaaa tcttttcctt cttctgatat
      301 tgtaaagttt ttgatgttgt tcggtgttcc aactcctatc gtctgctcta ctccttggta
      361 tgttgatgtc aaaagcacgt tatagtatcc agccctaaga ccaattggct caaatgaaac
      421 atggtatact ccattttcat cggatgtgat ttcttttgtc tgaagagttt ccatccactg
      481 tgcgttttgc aggtcatatc tttttatttc agctttgatt ggcacgtttg gtattataga
      541 gccgcatcca ttgtaagcag tctttgcagt aatgttgatt ggtttttctg tcataaatcc
      601 tggtccaaaa tcgtactgga atgggacaaa gaccgtataa ggaggatctt caggcaagtc
      661 tgttggaatt atttctattt gaaaatattc aaagctgctg acataaccat cgatgtcacc
      721 gcctacagat acctgcagat catacttgcc aggcattaca gtgtttgtaa taggaacatc
      781 tccttttacc acacctgagg accaatagtc tggctgtagt gaataaagtg gggagagatc
      841 aagatatgct ccagcataac cggatatttc ggtactgcac gtctcgtgtg gtatatcacc
      901 tgttacaaga attctggcat aatgagcagg attgccaaca ccatcaacca ctttggcctc
      961 aaactttact ggctcgcctt gttttagtac aggatttagc accttgatgt ctgaaatcac
     1021 aaacttgcta gcatcttttt ttgtaaaaaa tagttttgat tgtactccgg ctggcgattc
     1081 tagtgttaga agatacctgt atctgtcagg cgattgagga gtcttgtatt caaacatggc
     1141 cttgccatcc tccagtgttt tctcctcttg atgtatgacg tttgcataat ctggcccaaa
     1201 agtcacgtca gaaattttca ctataacatt ttcattcgaa ttctcatact gttgatctgc
     1261 cctgacagta attatgacag tgtcacctgt gttgtattct gttttgtcta gcttaaattc
     1321 aatgcaacaa ggataccaac caccaccaag ggctgtttgc gcatcaaaaa caattccaat
     1381 caaaatagca attgttacga ttatcaatat atgaaaaaat ttcatattct gttttattct
     1441 agcctgcttt ttagatatta cttacaaatt gcatctgtat tgattttgtg tttttataca
     1501 gacttttgca gcacatactt ttctttacct tttttgcttc taggtaagga acgcagtttt
     1561 ctcttgcagc acgggcatga tattccatca caattaatga aaatctcaca aaagttgcag
     1621 cgtttctgtc ctgctgcata ccggccaatt tcagaaggct ttagtgcctt gtacctagtg
     1681 cataggccca tgcagtatgt catttcagac ctcgaaattg tttgcttttt ttaatacttg
     1741 caaaaagaca cttgtcagag cagtgtgttg attgcgagtc tttcattact ttaccgcaat
     1801 attcacactt tttttccatt tcaaaaatct gaaagatctc ggtattagaa ggttggctga
     1861 ttatcaaagt tgataatgac agacatcaaa taaaaaactc ttttccttct agtccagctg
     1921 tgccccttga cataatggat gaaaccgatc tgagagacat tccaacaaga gagcgattga
     1981 acagatgcat ttacaccata aaaaatgatg cagatgaatc aaagaggtgg gatgcagtat
     2041 atttagctgg agaaatagca gaagcaaatc ccacagatcc aatctttgat gaggttgctg
     2101 aactaatgtc ttggattctg caaaacgatg acaacggagt gataaagcat gaagtgtgtt
     2161 ttcagattgc ggcaagaaac atgaggaaaa aaattccaga tctaatcaat acagccctaa
     2221 acgacaagag cgggatagca aaacatgaag cgctagagtg tctagctttg atgaaagcat
     2281 ttgaggcaaa agatacaatg ataaaagctc tcaaggatcc aatttctcat gtcagagaga
     2341 ctgcagcgtt tgctctaaaa agacttgata ggctggacaa aaaggcatac acgccatcaa
     2401 agatactttg attgtatcaa gatgtaatct cagtaagata cacgttccag attctcaact
     2461 ttggtagcaa atatctctca gttttttaag tgtggaaggg aacctaaatg gttttgaaca
     2521 gttgccaata cgtagatggt aggttaggtt ccacttctga gatgtcttga tggaattttt
     2581 atttaaaaaa tatcatgatt atcaagcttg gaaactggaa cttcatgcca atagtctgat
     2641 catctaatta gtgtcattcc cttagatgtt cctttttctc ttttccagat tcaaatatct
     2701 gtccaggaag gatgtctttg tgatcgtgtc ctatgtgcat tgtgtgctct agtaagcttg
     2761 taaatttttt attgcatatt ggacagtttt tctcaaatcc acccatgaat tgataaatga
     2821 aaaaacgtct gtttttactt cattatgatt atcaggcttg agaaccagag cttgactcgt
     2881 gacaattaat gaatcaaaag cactgggcag tgagagtgct ctgatacctt tcttgcaacg
     2941 cttccaattg cctttagtct tgaaaatcca cccaagcctc ttgttccaat cactataaga
     3001 tctatttctt tttctttt
//
LOCUS       DATIDL010000030         3255 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrosopumilaceae archaeon isolate SMAG_U16342
            k141_1254797, whole genome shotgun sequence.
ACCESSION   DATIDL010000030 DATIDL010000000
VERSION     DATIDL010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935836
            Sequence Read Archive: SRR25158326
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosopumilaceae archaeon (soil metagenome)
  ORGANISM  Nitrosopumilaceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrosopumilales;
            Nitrosopumilaceae.
REFERENCE   1  (bases 1 to 3255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/04/2024 05:09:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,520
            CDSs (total)                 :: 1,474
            Genes (coding)               :: 1,474
            CDSs (with protein)          :: 1,474
            Genes (RNA)                  :: 46
            rRNAs                        :: 1 (5S)
            complete rRNAs               :: 1 (5S)
            tRNAs                        :: 43
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3255
                     /organism="Nitrosopumilaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1254797"
                     /isolate="SMAG_U16342"
                     /isolation_source="soil"
                     /db_xref="taxon:2202732"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1129
                     /locus_tag="VLB45_01310"
     CDS             <1..1129
                     /locus_tag="VLB45_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016939685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HSD04376.1"
                     /translation="ESIKIQSTNKQTHLSIFLKARSLEQQGKKIIHMEVGEPDFYPPK
                     IVKKSLGEVYDKGFTKYGEAKGMTEFRAALAEKTSTRFKTKTKIENIMVSPGARFSVF
                     LTISTLLRPGDEIIVIEPAWPAYRENALNSGIKVRTISTRLEEKWEPSVEQISSIINP
                     NTKMIVLNYPNNPTGKILPTNLQDEIMQLAIKNNLYVLSDEIYADYSYGIWKSILSYE
                     YDKSIITQSFSKSHAMTGFRIGYAVSSPEIIDKMAKLQALCMTNVSEPIQYVALKALD
                     ADTSDNTKTMRNRLELVVEMAKNMQLDFVKPDGAMYIFARARKNNFDSTEFTNKLLDL
                     GVAIAPGEAFGNYQNFVRISACQPEKLLNEGMKVIAKALSE"
     gene            1131..1985
                     /locus_tag="VLB45_01315"
     CDS             1131..1985
                     /locus_tag="VLB45_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018039383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HSD04377.1"
                     /translation="MKKKIAIIGAEGQMGKWFSKYFIDKGFEVIGYDAEKEILNKSVT
                     KAKSLVGAILSVDYVMLCTPTKRTPEIIRLIAKEMKRESYLIDISSLKSKVVSALSKI
                     PDKVSPICIHPMFGPGTKKIKGHNIISIPIRDAKKEMAVIKSLFSEANFVTIDAAEHD
                     KKIAVILGMTHLINLVFANILAKDEKLSLTEKMSGTTFTAQKILTASIMTESPDLIET
                     IISNPEIRKYAEELWKDIGRMLTLIQENKSEEIMNYITTTKEKLSKSVDLEDSYKKLI
                     TMINAVEK"
     gene            1987..2391
                     /locus_tag="VLB45_01320"
     CDS             1987..2391
                     /locus_tag="VLB45_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010192934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HSD04378.1"
                     /translation="MHHTRIVTSFLTNDERFLILKRSQNVKSMKGLWGGVSGVIEENE
                     EPLRRAKIEIFEEVGIEERQIDLLKTATELQVRSEQYSNHEWTIFPFLFNVKEPKIRL
                     NWENSDYKWISPDEISRYKTVPNLDEVLARLL"
     gene            complement(2374..>3255)
                     /locus_tag="VLB45_01325"
     CDS             complement(2374..>3255)
                     /locus_tag="VLB45_01325"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HSD04379.1"
                     /translation="FERNIKDGVDYTTIHSGITKEIAKRILAVKRHGGIVSKGGTITA
                     AWMLKYDKENPYFEHFDYLIELARKYDVTFSLGDALRPGSILDSHDELQVQEMINISR
                     LTKQAHEKDVQVMVEGPGHVPLNEVAANVKLAKALIGEVPYYVLGPLVTDVASGHDHI
                     ASAIGAAISASEGVDLLCYLTPAEHLALPTPEEVKAGLIAYRIAAHAGDLVKLREKAI
                     RWDMEMTEARRTLNWEKQIALSIDPEEAAKIHGRVGQINGNTVPCTMCGGACVYIMLP
                     QQRSNTPEEVAKLQQSS"
BASE COUNT         1103 a          626 c          638 g          888 t
ORIGIN      
        1 cgaatcaata aaaatccaat ctaccaacaa gcagactcac ctttccatat ttttaaaagc
       61 aagatccttg gagcagcagg gaaaaaagat catccacatg gaagttggcg aaccagattt
      121 ttacccgcca aagattgtca aaaaatctct tggcgaagtg tacgataagg gatttacaaa
      181 atatggcgaa gcaaagggaa tgacagagtt tcgtgcggca cttgcagaaa aaacttcaac
      241 aagattcaag acaaagacaa aaatcgaaaa catcatggtg tccccgggtg caagattttc
      301 cgtgtttctt acaatttcaa ctcttttgcg tcccggcgac gagataatag tgatagagcc
      361 agcatggcct gcatatagag aaaatgcgtt aaactctggc atcaaagtaa gaaccatcag
      421 tactagactg gaagaaaagt gggaaccatc agtagagcag atttcaagca taataaatcc
      481 aaataccaaa atgattgttc ttaactatcc aaacaatcca acaggcaaga ttcttccaac
      541 aaaccttcaa gatgaaataa tgcagcttgc aatcaaaaac aatctgtacg ttctaagcga
      601 tgaaatctat gcggattatt cttatggaat ctggaagagc atactttcgt atgagtatga
      661 caagagcatc ataacgcaat cgttttcaaa atctcatgcc atgacaggct ttaggatagg
      721 ttatgctgtc tcaagtcctg aaatcataga caagatggca aaacttcagg cactttgcat
      781 gacaaacgta tctgaaccaa tccagtatgt ggccctaaag gcacttgatg cggatacaag
      841 cgataatact aaaaccatga gaaacagact agaattagtg gtcgagatgg caaaaaacat
      901 gcagttagac tttgtaaaac cagatggggc aatgtacatt tttgccagag caagaaaaaa
      961 taattttgat tctactgaat ttacaaacaa attacttgat ctaggagtgg caattgcgcc
     1021 aggtgaagca tttggtaatt atcagaactt tgtaagaatt tctgcgtgcc aaccagaaaa
     1081 attactaaat gaaggaatga aagtcatagc caaggcgctg agtgaatagg atgaagaaaa
     1141 aaattgcaat aattggggcc gaagggcaga tgggcaaatg gttttcaaaa tattttatcg
     1201 acaaaggttt tgaggtaatc ggatatgatg cagaaaaaga aatactcaac aagtctgtga
     1261 ccaaggcaaa gtcacttgtc ggtgcaattc tttcagtgga ttacgtcatg ctttgcactc
     1321 caacaaaaag aactcctgaa atcatacgat tgatagcaaa ggaaatgaaa agagagtcat
     1381 atcttatcga tatttcgtca cttaaatcca aagtggtatc tgcactttca aaaatcccag
     1441 acaaggtgag cccaatatgc atacatccca tgtttggacc tggaacaaaa aagatcaagg
     1501 gccacaatat aatttccatc cctatccgtg atgcaaaaaa agagatggct gtaataaagt
     1561 cgctttttag cgaagcaaac tttgttacaa tagatgccgc agagcatgac aaaaaaattg
     1621 ccgtgatttt aggcatgact cacctcataa atctcgtatt tgcaaacata ttggcaaaag
     1681 atgaaaagtt gtccctcact gaaaaaatgt ctggaacaac atttaccgca caaaagatac
     1741 tcacagcaag cataatgact gaatcgccgg atcttattga aacaataatt tcaaacccag
     1801 aaataagaaa gtacgctgaa gaactttgga aggacattgg caggatgctt actcttattc
     1861 aggaaaacaa gtcagaagaa ataatgaatt acattactac caccaaagaa aagctttcaa
     1921 agagtgttga tttggaagac tcttacaaga aattaattac catgataaat gctgtagaaa
     1981 agtaatttgc atcacacaag gatagttacg tcatttctta caaatgacga aaggtttctt
     2041 atcttaaagc gcagccagaa cgtaaaaagc atgaaagggc tttggggtgg agtaagtgga
     2101 gtaatagagg aaaatgaaga acccctgcgc agagcaaaaa ttgagatatt tgaagaagtt
     2161 ggaattgaag aaagacaaat tgatcttttg aaaacagcaa cggagttgca agttagatcc
     2221 gagcaatatt ctaatcacga gtggacaatc tttccatttt tgtttaatgt aaaagagcct
     2281 aaaataagac tgaactggga aaattctgat tacaagtgga ttagtccaga tgaaatttca
     2341 agatataaaa ctgtaccaaa ccttgatgaa gttctagcta gactgttgta gttttgccac
     2401 ttcttctgga gtgttgcttc tttgctgtgg aagcatgatg tagacacagg cacctccaca
     2461 cattgtacat ggaaccgtgt ttccatttat ctgacctacc ctgccgtgta ttttggcagc
     2521 ctcttctgga tctattgaga gagcgatttg tttttcccag ttgagagtac gtctagcttc
     2581 agtcatttcc atatcccatc tgattgcttt ttcgcgaagt tttaccaaat ctcctgcatg
     2641 tgcagctatt ctgtatgcaa tcagtccagc ctttacttct tctggagtag gcaatgccaa
     2701 atgttctgca ggtgtaagat aacataatag atccacgcct tcgcttgctg aaattgcagc
     2761 tccaattgca cttgcaatgt gatcgtgacc tgatgccacg tcagtaacaa gagggcccag
     2821 aacataatat ggaacttcgc caatcaatgc ttttgctagt ttaacgtttg ctgcaacttc
     2881 atttaatggt acgtgacctg gcccttcaac catcacttgg acatcttttt catgagcttg
     2941 ttttgtgagc cgtgaaatgt tgatcatttc ttgcacttga agctcatcat gtgaatcaag
     3001 aatagagcct ggtcgcagtg catcacccaa gctaaaagtc acatcatatt ttcttgcaag
     3061 ctcaattaga taatcaaagt gttcaaagta tggattttct ttatcatact ttagcatcca
     3121 tgccgcagtt attgttccgc ccttacttac aatgccaccg tgtcttttta ctgcaagaat
     3181 tctttttgca atctcttttg taataccaga atgtatcgta gtgtaatcaa cgccatcttt
     3241 tatgttcctc tcaaa
//
LOCUS       DATIDL010000031         2672 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrosopumilaceae archaeon isolate SMAG_U16342
            k141_650085, whole genome shotgun sequence.
ACCESSION   DATIDL010000031 DATIDL010000000
VERSION     DATIDL010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935836
            Sequence Read Archive: SRR25158326
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosopumilaceae archaeon (soil metagenome)
  ORGANISM  Nitrosopumilaceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrosopumilales;
            Nitrosopumilaceae.
REFERENCE   1  (bases 1 to 2672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/04/2024 05:09:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,520
            CDSs (total)                 :: 1,474
            Genes (coding)               :: 1,474
            CDSs (with protein)          :: 1,474
            Genes (RNA)                  :: 46
            rRNAs                        :: 1 (5S)
            complete rRNAs               :: 1 (5S)
            tRNAs                        :: 43
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2672
                     /organism="Nitrosopumilaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_650085"
                     /isolate="SMAG_U16342"
                     /isolation_source="soil"
                     /db_xref="taxon:2202732"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2067
                     /locus_tag="VLB45_01330"
     CDS             <1..2067
                     /locus_tag="VLB45_01330"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed DNA polymerase I"
                     /protein_id="HSD04380.1"
                     /translation="KGLLWDKVTNIGMINPKEFQDHISDWANLLNQTIPKIRRVSFDI
                     EVESEVGRIPDPKVAEKKITAMGFSGSDGMKQVFVLKRKDKKLGTSELASDIKVTFYE
                     EDQEKEMIKDGLKVLTDYPFVITFNGDEFDMPYVYNRADRLGMESKDIPLIMMRDSAT
                     LKHGVHLDLYRTFSNRSFQIYAFSHKYADFSLNSVSEGLLNESKIEYDGELEDLTLYE
                     LANYCYNDARLTQMLTSFNNDLLMNLLVVITRIGRMPIDDISRMGVSQWIRSLFYFEH
                     RKHNFLIPRRDELDQKSTAVSIDAIIKDKKYRGGLVVEPLEGIHFDVVVMDFASLYPS
                     IIKVRNLSYETVRCPHQECKTNTIPETTHWVCTHKNGLTSLLIGSLRDLRVNYYKNLS
                     KNQSLGEDQKQLYTVVSQALKVILNASYGVMGAEIFPLYCLPVAEATTAVGRYTILQT
                     IEKCKSVGIEVLYGDTDSLFLKGPTKDQVQIVIDWAKKEFGVDLDLDKEYRYVVLSTR
                     KKNYLGVQKDGKVDVKGLTGKKSHTPPFIKTLFYEILEILSKVKTKEEFAESKEKISN
                     MISQYAKKLKAKQIPLSELTFNVMISKAPSEYVKTIPQHIRAAKLLESIREIKKGDII
                     SYVKIINKPGVKPVEMARPDEIDSAKYMEFMESTFDQILSSMDIDFGTLIGAPKQTGL
                     DQFFWN"
     gene            2450..>2672
                     /locus_tag="VLB45_01335"
     CDS             2450..>2672
                     /locus_tag="VLB45_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HSD04381.1"
                     /translation="MSFLDLYMSKSPLIASPNDAGESSVIVYGVPFDSTHSFRPGTRF
                     GPDAIRLALNNIEIFSTRFNVDLESIHIED"
BASE COUNT          919 a          473 c          542 g          738 t
ORIGIN      
        1 aagggtctgc tgtgggataa ggttacaaac attggaatga tcaatcccaa agaatttcaa
       61 gaccacatct ctgactgggc aaacctgcta aatcaaacca tcccaaaaat caggcgagtt
      121 agctttgaca ttgaggtaga atctgaggta ggaaggattc ctgacccaaa agtggctgaa
      181 aaaaagatta ccgccatggg attttctgga agtgatggaa tgaaacaggt ctttgtacta
      241 aaaagaaaag acaaaaagtt aggcacaagt gagcttgcgt cagacatcaa agtcacattc
      301 tacgaggaag accaagaaaa ggaaatgata aaagatggcc tgaaagtttt gacagactat
      361 ccatttgtaa ttacattcaa cggggatgaa tttgacatgc catatgtgta caacagagcg
      421 gacaggcttg ggatggaatc aaaagacatt ccgcttatca tgatgcgtga ctctgcaaca
      481 ctaaaacacg gtgtacatct tgatctgtac aggacatttt caaatcgctc tttccaaatt
      541 tacgcattta gtcacaagta cgctgacttt tctttaaaca gcgtgtctga agggttgtta
      601 aatgaatcaa aaatagaata tgatggcgag cttgaagacc ttacactgta cgagcttgca
      661 aattattgtt acaacgatgc aagactgacg cagatgctga caagcttcaa caatgatctt
      721 ctgatgaact tgcttgttgt cataacaaga attggaagaa tgccaattga tgacatttca
      781 agaatgggtg tctcgcaatg gattagaagc ttgttttatt ttgagcacag aaaacataat
      841 tttctaatcc cgcgcagaga cgagcttgat caaaaatcta ctgcagtgtc aattgacgcg
      901 ataataaaag acaaaaaata ccgcggagga cttgttgttg aaccgctaga gggaatacac
      961 tttgacgtag ttgtaatgga ctttgcaagc ctgtatccta gtataatcaa agtgcgtaat
     1021 ctgtcctatg agacagtgcg gtgcccgcac caggaatgca aaacaaacac cattcctgaa
     1081 accacgcact gggtatgtac gcacaaaaac ggcttgacct cactcctgat tggctcgcta
     1141 agagatctcc gagtaaatta ttacaagaat ctctcaaaaa accaatcctt gggtgaggac
     1201 caaaagcagc tttacactgt ggtaagccag gcactcaaag tcatacttaa tgcaagttat
     1261 ggtgtcatgg gtgcagaaat ctttccattg tattgtttgc ctgttgcaga agccactaca
     1321 gctgtgggaa gatatactat tttgcaaaca atcgaaaaat gtaaatctgt tggaatcgaa
     1381 gtcttgtatg gggatactga ctcattattt ttgaaaggtc cgacaaaaga tcaagtccaa
     1441 atagtaattg attgggcaaa aaaagaattt ggagttgatt tggatcttga taaagagtat
     1501 agatatgttg tactaagtac tagaaagaaa aactatcttg gcgtgcaaaa ggatggcaaa
     1561 gttgatgtca agggattgac tggaaaaaaa tcacacactc caccatttat caaaacatta
     1621 ttttatgaaa ttttagaaat tttatccaaa gtaaaaacaa aagaagaatt tgctgaatct
     1681 aaagaaaaaa ttagtaacat gatatcgcaa tatgcaaaaa aattaaaagc aaaacaaatt
     1741 ccactttctg agcttacttt taatgtgatg ataagcaaag caccatctga atatgtaaaa
     1801 accattccgc aacacatcag ggcggcaaaa ctgttagagt ccatccgcga aataaaaaag
     1861 ggcgatatta tttcttatgt gaaaataata aacaagcctg gcgtaaagcc agttgaaatg
     1921 gcacggccag acgagattga ttctgcaaaa tatatggaat ttatggaaag cacgtttgac
     1981 caaatactat cttcaatgga tattgacttt ggcactctaa ttggggctcc aaagcagaca
     2041 gggctggacc agttcttttg gaactaagcc aatcattcta cactcttagg tgtaattagt
     2101 taaggctaat tttcacagaa tttataagag gatcttgcaa tttctataaa catgcgaata
     2161 gattcatgta ggacatgtgg cgaagatatg caaaaatttc aaacatgccc agtatgcagg
     2221 gaaaccacgc gttatgtttg ccgcaagtgt ggtaatacta gcgaagaaca ggttcactca
     2281 gaatgtttgc tagtgaacaa atcagtagtt gctaactagt ttcaagtttc ttttcaaaca
     2341 atttctgtaa agttatctgt tggtttacgg tatcgtatat cctaccaaat cacacgctga
     2401 atattttttc aatatctaaa tatgtaggta gcagactcac taaacaaaaa tgagttttct
     2461 tgatctttac atgagcaaaa gtccattgat tgctagtcca aatgatgctg gagagtcttc
     2521 tgtaattgta tatggagttc catttgactc cacgcattct tttagaccag gtaccagatt
     2581 tggtcctgat gcaatcagac tggcattaaa taacattgag atattttcaa ctagatttaa
     2641 tgttgatttg gaatctatcc acattgaaga tc
//
LOCUS       DATIDL010000032         8001 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrosopumilaceae archaeon isolate SMAG_U16342
            k141_2669990, whole genome shotgun sequence.
ACCESSION   DATIDL010000032 DATIDL010000000
VERSION     DATIDL010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935836
            Sequence Read Archive: SRR25158326
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosopumilaceae archaeon (soil metagenome)
  ORGANISM  Nitrosopumilaceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrosopumilales;
            Nitrosopumilaceae.
REFERENCE   1  (bases 1 to 8001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/04/2024 05:09:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,520
            CDSs (total)                 :: 1,474
            Genes (coding)               :: 1,474
            CDSs (with protein)          :: 1,474
            Genes (RNA)                  :: 46
            rRNAs                        :: 1 (5S)
            complete rRNAs               :: 1 (5S)
            tRNAs                        :: 43
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8001
                     /organism="Nitrosopumilaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2669990"
                     /isolate="SMAG_U16342"
                     /isolation_source="soil"
                     /db_xref="taxon:2202732"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..309
                     /locus_tag="VLB45_01340"
     CDS             <1..309
                     /locus_tag="VLB45_01340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04382.1"
                     /translation="RGILVTPIDEIPAEASSFSVPTKVISVGGADVQVFEFASEQDAV
                     AATQTVSEDGTEIGTSIIRWIGPPHFYTQGKLIVLYVGDNSEVTSLLEDFIGPQFAGF
                     "
     gene            complement(337..981)
                     /locus_tag="VLB45_01345"
     CDS             complement(337..981)
                     /locus_tag="VLB45_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair helicase"
                     /protein_id="HSD04383.1"
                     /translation="MALSDISFKQAYRSDRDDLVSEFFIPCLSNCIQFDRSIQHVTVK
                     SVSTLSAGFKNFAKNEAKIRMVTGHRFKPNELTLLSKIFYNEKTEKKPIEHNIENGNE
                     VEILKQIIHNNKLEIKIAVPNQNEFSGYFSENMGIFRDSENNVVAFTGTSTETFTNQD
                     RNFESLDVFTSWSDKSRVETKIADFENLWSNKTKYVNVCDFSYAERNNLLKYIV"
     gene            1040..1255
                     /locus_tag="VLB45_01350"
     CDS             1040..1255
                     /locus_tag="VLB45_01350"
                     /inference="COORDINATES: protein motif:HMM:NF025005.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KTSC domain-containing protein"
                     /protein_id="HSD04384.1"
                     /translation="MERVRVKSGTIRAIGYDEAEQILEIQFVHGGIYHYFGVSKKIYD
                     SLMRAPPAYGTYHARYIKNRYRHKKIA"
     gene            1311..1628
                     /locus_tag="VLB45_01355"
     CDS             1311..1628
                     /locus_tag="VLB45_01355"
                     /inference="COORDINATES: protein motif:HMM:NF024645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HSD04385.1"
                     /translation="MTFCPSCGNEVPSESSFCPKCGKQIGTNASGVSKSRSKWWFLLP
                     ILFHVIGGVIVYFVIRDDDPKLAKNCLWLGIIISAIEVVFVVAFWIPFGMTRHMGWNG
                     FML"
     gene            1715..2773
                     /locus_tag="VLB45_01360"
     CDS             1715..2773
                     /locus_tag="VLB45_01360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04386.1"
                     /translation="MKPIQQNLLVVIGCIIVALVILAVFSVYSTKVPLQRQAIHVSND
                     QSLKLSNPNGLIKLSEIKPNSMTWFWYPDPDRFKDRDPFDLFLLIRLPDYLGGTVNDA
                     SALRAYSAIDPSSHCIVKYWPEEGRRRIEDPCGGNMYEPMYGHAIQIGGNPILVSENL
                     GLPYLTVSSDKDGYLYVEPPIWTEDKNGVIGIGRRVSAQEIESANELITTRENQIKDA
                     LEKFHVPDKLSTGHSLSLISNDGIRTNHAMYFDPNSDQSTTLSTIMLYYEYCNCTKSK
                     ELLADEEGKTQSQLVEIDDIPIVAYPIAVNYLTGIHDKYVFVFYNNGYKISLYTNQKM
                     EPGLNLVKEIIDYENKRS"
     gene            2930..4192
                     /locus_tag="VLB45_01365"
     CDS             2930..4192
                     /locus_tag="VLB45_01365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Hint domain-containing protein"
                     /protein_id="HSD04387.1"
                     /translation="MGHPLEFWDSADIILDGTVISTHLNETDKLVQHDVRVEQYFKNP
                     KPEKMITVYGPDTYNEDWFYPKFFKEGERALFYLKKIGDKYIILEQSRAATKECSPRD
                     MIGLSTLPSEPVGRGGPTLLFDPYQTCNGYLYSVDYLSRTLKPLKQFEAGIKLEDIRC
                     AEDRVMVVKIDDTPACVKPSSIQRLFDSGWAKPIDSTDGTEITPGPGETLVTLKEGQK
                     EGSLLVQKIFQDSVSGLNFPEYPIATNSGLPLTLHLGDTASNGCTVEMTLVKISGNTA
                     IFLKKEHNDRPCPICLSEDTVIDTPNGVINVKEVKEGMMIFTQDASGHRYIGTVLQAG
                     KTMVPTDHKMVHIILDDKRELHVSPNHPTADGRLFGELLIGDTLDGSKIKNTELVLYN
                     GTYTYDILPSDQTGFYWANGILIKSTLK"
     gene            complement(4468..4905)
                     /locus_tag="VLB45_01370"
     CDS             complement(4468..4905)
                     /locus_tag="VLB45_01370"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HSD04388.1"
                     /translation="MEDKKLIIAGIVGGLVLLASLSAFVGFGNVRHTPIFWQETIKST
                     VAFEKIDGQTRLVGVEGNSEINPTLVMRADGTLYILTIINRDDIPHMFYVEGLDATSK
                     VLRAGENDTITLVTKNEGTFSYYDRFDQKPLGVIKAVKISTFD"
     gene            5041..5409
                     /locus_tag="VLB45_01375"
     CDS             5041..5409
                     /locus_tag="VLB45_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HSD04389.1"
                     /translation="MPRIIHFDVPADDVARAQKFYGEVFGWKFEKWNGPMEYWMTITG
                     DDKQPGINGGMSKRMPGQMGVTNTIDVSSVDEFSKKIQSKGGTIIMPKMAIPGVGYFA
                     QCMDTEGNVFGIIQLDKNAS"
     gene            complement(5431..5787)
                     /locus_tag="VLB45_01380"
     CDS             complement(5431..5787)
                     /locus_tag="VLB45_01380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04390.1"
                     /translation="MNKVAVFVFAEPGTKESLGRVVNAMITVKELKEAGNDVRLYFDG
                     TGTKWPKELSKKDHIAYGLHEAVKDKVAGACLFCATAFGAKDDVKDCKVKLVDEFDQH
                     ISIKKLLSEGFSIMNF"
     gene            5883..6260
                     /locus_tag="VLB45_01385"
     CDS             5883..6260
                     /locus_tag="VLB45_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012216043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HSD04391.1"
                     /translation="MDSKSNLKSCPIHNTFDLIGKKFTILILRNMILYGQTRFNKLLD
                     SVEEINSKTLSLRLREMEHDGLIKRHVYAEIPPRVEYELTEKAKNLKPILEQMGEFST
                     RYCASDVFKDKRPRSFKQVIDAL"
     gene            complement(6298..7209)
                     /locus_tag="VLB45_01390"
     CDS             complement(6298..7209)
                     /locus_tag="VLB45_01390"
                     /inference="COORDINATES: protein motif:HMM:NF012703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HSD04392.1"
                     /translation="MKQKNNDLKLSLIGVPTNSAGSKNGVARAPQVLRQAGLVDALSR
                     HCEVRDEGDVTFLEPVLQRHDKYQIIGYDSFVSMVYSVQRSVSKVLQEGRFPLVIGGD
                     CSILLGCLAATKGAYEKFGLFFIDGHEDAYPPQKSPTGEAADMELGFALGKNIDKLTR
                     EFTKFLPLIDKTNVCLFGPRDKKLLQNTNVESLADEINCYDDTIIHNNSIENLINNAI
                     ANLRTRTWWTHIDFDVLSTDSMPAVDYKQPGGISWRELYQLSIPLLSQGCAGCNITIY
                     NPDLDPDMHHAKRIIKYIEDVIPTINK"
     gene            7445..7966
                     /locus_tag="VLB45_01395"
     CDS             7445..7966
                     /locus_tag="VLB45_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DM13 domain-containing protein"
                     /protein_id="HSD04393.1"
                     /translation="MNKKALVAIIVAAVVVPIGAYAVSPLFINNTIDEPLPTSEKMMA
                     DGDSMDKVSSDSMKDGMEKESMMDKSFSGTFVGVDAVHDAKGMAKIITLDDGSDVLRL
                     EDFRSTNGPDLYVYLATDETASDFVNLGRLKANIGNQNYNIPEGTDLSKYDSVLVWCK
                     QFSVLFGKAELSS"
BASE COUNT         2555 a         1528 c         1535 g         2383 t
ORIGIN      
        1 cgaggcatct tggtaacccc aatagatgaa attccagctg aagcctcatc gttttccgta
       61 ccaaccaaag tgatctctgt tggaggagca gatgtccaag tctttgagtt tgcaagtgaa
      121 caagatgcag tagctgcaac acaaactgtt tcagaagacg gaacagaaat tggaacaagt
      181 ataatacgct ggataggccc accgcacttt tacacgcaag gcaagctaat cgtcctatat
      241 gtaggggata attcagaggt aactagtctg cttgaggact ttattggtcc acagtttgca
      301 ggattttaaa accctgaatc ataccttttt ttaaatttat acgatatatt ttaaaagatt
      361 atttctttct gcatagctaa aatcgcacac attaacatat tttgtcttgt tgctccacaa
      421 gttctcaaag tctgcaatct ttgtctccac ccttgatttg tcactccacg aagtaaagac
      481 atcaagtgac tcaaagtttc tatcttgatt agtaaaagtt tcagttgagg tcccggtaaa
      541 tgcaactaca ttattctcag aatctctaaa gattcccatg ttctcagaaa agtatccgct
      601 aaactcgttt tggtttggca ctgcgatttt tatctcaagc ttgttgttgt ggatgatttg
      661 ctttaggatc tcaacctcat taccgttttc aatgttgtgt tctattggtt ttttctctgt
      721 cttttcattg tagaaaatct tggataacag cgtaagctcg tttggcttga acctgtgtcc
      781 tgtcaccatt cttattttag cttcattttt tgcaaaattt ttaaagcctg ctgaaagcgt
      841 gctgacactt ttgactgtaa catgctggat tgacctgtca aactggatgc agttgctaag
      901 gcacggaatg aaaaactcag ataccaagtc gtccctgtct gatctgtatg cttgtttaaa
      961 ggaaatgtct gataaagcca ataaaagaca aaatgtctag tttattattt aaatcaagac
     1021 gggttataca attagtaaga tggaaagagt acgagtaaag tctggtacca tcagagcaat
     1081 aggatatgat gaggcagaac aaatcctgga aatacaattt gtccatggcg gaatttatca
     1141 ttattttgga gtatcaaaaa aaatctatga tagtttaatg cgcgccccgc cagcttatgg
     1201 gacataccac gccagataca taaaaaacag ataccgccac aaaaaaatcg cctaaaccat
     1261 acaaaatatc ttgttcttga tttttattat agatgcaaaa aagtataacc atgacatttt
     1321 gcccttcttg cggaaatgag gtgccttctg aaagcagttt ttgtccaaag tgtggaaaac
     1381 aaattggaac aaacgcttct ggtgtatcaa agtcaagaag caaatggtgg tttttacttc
     1441 caatactgtt tcatgtgata ggcggcgtga tcgtttactt tgtaatacga gatgatgatc
     1501 caaagcttgc caaaaattgt ctctggcttg gaataataat cagcgccatc gaagttgtat
     1561 ttgttgttgc gttttggatt ccatttggca tgacaagaca catgggctgg aatggcttta
     1621 tgctctaatc ttgactagtc atcaggccat ttgaaatttt atcatataca atttatcagc
     1681 aacccctaaa ggcattaaag tacacatgat aaatatgaaa ccaattcaac aaaatctttt
     1741 ggttgtaatt ggttgcatta ttgttgcact tgtaatttta gctgtatttt cagtatattc
     1801 taccaaggtg ccattacagc gtcaggcaat acacgtctcc aacgatcaaa gcttaaagtt
     1861 atcaaatcca aacggcttga tcaaactttc tgaaataaaa ccaaacagca tgacttggtt
     1921 ttggtatcca gatccagaca gattcaaaga cagggatcca tttgatctat ttttgttaat
     1981 caggctgcct gactatcttg gaggaacagt aaatgacgct tctgcgctta gagcatacag
     2041 cgccattgac ccttcatcgc actgtatagt aaaatattgg ccagaggaag gacgaagaag
     2101 aatcgaagat ccttgtggag gaaacatgta tgaaccaatg tatggtcatg caatacaaat
     2161 tggcggaaat ccaatcctgg tctctgaaaa tcttggactg ccatatctga ctgtgtcttc
     2221 agacaaggac ggttacctgt atgtagagcc accaatatgg actgaggata aaaacggggt
     2281 aatcggtatc ggaagaagag tctctgcgca agagatcgaa tcagccaatg aattaatcac
     2341 aacaagagaa aatcagatca aagatgcgct agaaaaattt catgttcctg acaagctctc
     2401 aactgggcat agcctaagtc tcatcagtaa tgatggaata aggacaaacc atgcaatgta
     2461 ttttgatcca aattctgacc agtctacaac tttgtctaca atcatgttat attatgaata
     2521 ttgtaactgt acaaaatcaa aagaactttt agcagacgag gagggaaaaa cccaaagcca
     2581 gcttgtagaa attgatgata tcccaatagt agcttatcca attgccgtga attatcttac
     2641 tggaatacat gacaagtacg tatttgtttt ttataacaat gggtacaaaa tatcacttta
     2701 tacaaatcaa aagatggagc ctggcttgaa tcttgtcaag gaaataatcg attatgagaa
     2761 caagagatct tgaaaggaat caagctgcta gttttaatca tttacaaaca atttatgagt
     2821 aatctctaaa aactactaat gacaaaaata taatacgaaa atctttctta ttgtcacaat
     2881 tgcaagtttt ataattctat caatgctgcc acatgctcaa ggtctcatca tgggacatcc
     2941 tttagaattt tgggacagtg cagacattat tttagacgga actgtaattt ctacacatct
     3001 taatgaaact gataaacttg ttcaacatga tgtaagagtt gaacaatatt tcaaaaatcc
     3061 aaaacctgag aaaatgatca ctgtttatgg tcctgatacc tacaacgaag attggtttta
     3121 tcctaaattt ttcaaggaag gtgaaagggc actattttat ctcaaaaaaa taggtgacaa
     3181 gtacataatt ttagaacaat ccagagcagc tacaaaagaa tgcagtccta gagacatgat
     3241 aggattgtct actctcccaa gcgaaccggt aggcagaggt ggacctactt tattatttga
     3301 tccatatcag acctgcaatg gttatcttta ctcggttgat tatttgtcaa ggacattaaa
     3361 gccattaaag caatttgaag ctggaattaa actagaagac attagatgtg cagaagatcg
     3421 tgtgatggtt gtaaaaattg atgacactcc agcatgcgtg aaaccatcta gcatccagcg
     3481 tctgtttgac agtggctggg caaaaccaat agattcaact gacggtacag aaataactcc
     3541 gggacctgga gaaactcttg taacactaaa ggagggtcaa aaagaaggat cactgcttgt
     3601 tcaaaagatt tttcaagata gtgtcagcgg tttaaatttt ccagaatatc caattgcaac
     3661 gaatagtgga ctgccgttaa cattgcatct tggagatact gcaagcaatg gttgcactgt
     3721 agagatgact cttgtaaaaa ttagtggcaa tactgcaatc ttcctaaaaa aagaacataa
     3781 tgacaggcca tgtccaatct gcctttcaga ggatactgtg attgatacac caaatggtgt
     3841 catcaatgtc aaggaggtaa aagaaggaat gatgatattc acacaggacg cctctggtca
     3901 caggtacatt ggaaccgtac tgcaggctgg aaagactatg gttccaactg accacaagat
     3961 ggttcatata attttagatg ataaaagaga gttgcatgtt tctccaaatc acccaacagc
     4021 agatggaaga ctgtttggag aattactgat aggcgatact cttgatggtt ccaagataaa
     4081 aaatacagag ctggtcctat acaacgggac atacacatat gacatattgc cttcagacca
     4141 aactggattt tactgggcaa acggaatttt gatcaagagt acactgaagt gaacgttgtt
     4201 tctaccacaa tcattttcaa ataggtttgt aagcaacctc taaaagtaat gcctgacgta
     4261 gatcaggtac aaaaattcta atgtaaatta tgattattta gaattgattg catcccaaca
     4321 gcagcattca tttcgtgtac agttgggaca caattttgct aactggctat ccttgcattc
     4381 tttgggaaga caattgcaat ctgcacattt tatttgcaat aacgatctta tctataccag
     4441 taataaaaag ataaaactac attctgtcta atcaaacgta ctaattttga ctgcctttat
     4501 tactccaagt ggcttttgat caaatctgtc atagtatgaa aaagtgcctt cattttttgt
     4561 aaccaatgtt atcgtgtcat tttctccagc tcgcaaaacc ttgcttgttg catctaatcc
     4621 ctcgacataa aacatgtggg gaatgtcatc gcggtttatt atagtaagga tgtacagggt
     4681 tccgtctgct ctcatgacaa gtgttggatt tatttcagaa tttccctcaa cgccaaccag
     4741 tcttgtctgt ccgtctattt tctcaaaggc aacagtgctc ttgattgttt cctgccaaaa
     4801 aattggagtg tgccttacgt ttccaaagcc aacaaaggca gaaagactgg caagtaagac
     4861 caatcctcct acaatacctg caatgattag tttcttgtcc tccaactatt tgacgaggcc
     4921 tgaatctctt attatgatca agagtctgtt ttcatctttg ataatcattt tcaaataggt
     4981 ttgtaagcaa tctctaattt ggaacaaaga aaataaaccc catcaaagaa aacaagatac
     5041 atgccaagaa taattcactt tgacgtacca gcagacgatg ttgccagagc acaaaaattc
     5101 tatggcgaag tctttggctg gaagtttgaa aaatggaacg gcccaatgga gtactggatg
     5161 acaataacag gagatgacaa gcagccagga atcaacggag gaatgtcaaa aagaatgcca
     5221 ggccaaatgg gagtaacaaa caccattgat gtatcatctg ttgacgagtt ctcaaaaaag
     5281 atccagtcaa aaggcggaac aataatcatg ccaaagatgg ccattccagg agtaggatat
     5341 tttgcccaat gcatggacac agaaggaaac gtgtttggta taattcagct tgacaaaaac
     5401 gcttcatgat aaaaaaagag ttttgcatta ttagaaattc ataatgctaa aaccttctga
     5461 caagagtttc tttatgctga tgtgttgatc aaactcgtct acaagtttga ctttacagtc
     5521 tttgacgtca tcttttgcac caaatgctgt tgcgcaaaac aaacaagctc ctgccacctt
     5581 gtcttttact gcctcatgca atccatatgc gatgtggtct ttttttgaga gctcctttgg
     5641 ccactttgtt cccgtaccat caaaataaag gcgaacatca ttccctgctt cttttagctc
     5701 ctttactgtt atcattgcat tgactacacg acccaagctt tcttttgttc cgggctctgc
     5761 aaacacaaac accgctactt tgttcatctt ttcacctcct tacctttcat aactagatga
     5821 agaaaggtaa tctatattaa ttagcaagta acaaaattgt aggtaggtga aaaataggta
     5881 acttggattc taaatctaat ctcaaatcat gtcccataca caacacgttt gatctaatag
     5941 gaaaaaaatt cactatccta atcttgcgca acatgatact gtatggacag accagattca
     6001 acaagctttt ggattcagta gaagaaataa actcaaagac cctgtcgttg aggctgcggg
     6061 agatggagca cgacggatta atcaaaagac atgtctatgc tgagattccg ccaagagtag
     6121 agtacgagct aactgaaaag gccaagaacc taaaaccaat actggaacag atgggcgagt
     6181 tttcaacaag atattgcgca tctgatgttt tcaaagacaa acgccccaga agcttcaaac
     6241 aagtcataga tgctttgtaa ttcttgcact agaattgagt ttgcataaag aatttggtta
     6301 tttgttaatt gtaggtatga catcttcaat gtatttgatg atgcgtttgg cgtgatgcat
     6361 gtcaggatcc aaatcaggat tgtaaattgt aatgttgcat cctgcacatc cttgggacaa
     6421 taatgggatt gaaagctggt aaagttctct ccaactgatt ccgcctggtt gcttgtaatc
     6481 tactgctggc atagaatcag tcgataatac gtcaaaatct atgtgtgtcc accacgttct
     6541 cgttctaaga ttagcaattg cattatttat taaattttca atactgttgt tgtgtatgat
     6601 cgtatcatca taacaattaa tctcatctgc aagtgattcc acatttgtat tctggagtaa
     6661 ttttttatca cgcggaccaa acaagcagac atttgtttta tcaattagcg gtaaaaattt
     6721 ggtaaattct ctagtaagtt tgtcaatgtt tttgcctagt gcaaagccaa gttccatatc
     6781 tgcggcctct ccggtaggag atttttgtgg tggatatgca tcctcatgtc catctatgaa
     6841 aaacaagcca aatttttcat aagcaccttt tgttgcagcc aaacacccca gaagaataga
     6901 acagtctcct ccaatcacta acggaaacct accttcttgt agcactttgc tgacactgcg
     6961 ttgaacagag taaaccattg aaacaaacga gtcgtatccg ataatctggt atttgtcatg
     7021 tctttgcaaa acaggctcca aaaatgtcac gtctccctcg tcacgtactt cacaatggcg
     7081 actcaacgca tctaccaaac cagcttgcct gagaacttgg ggcgctcttg ctaccccatt
     7141 tttgctgcct gctgagtttg taggaactcc tatcaaactt aatttcaagt cattgttttt
     7201 ttgtttcata atgatcgcaa ataattattc cagtttgtac aatgaacgat tccagattca
     7261 atctacttga ttagctgtaa caagtataaa aattgggaaa taactttttt gtaagtaaat
     7321 gaaaatacat cactagatta cgataaggtt accatgacat tttcctgtgt acataaaaaa
     7381 tttgggtgtg atttggacat gaattggaaa aatgtctttt atttattgta caaaccttgc
     7441 tttgatgaat aaaaaagcgc ttgttgccat aattgtagct gcagttgtag tgcccatagg
     7501 tgcatatgct gtctccccac tattcatcaa caataccata gatgagcctc tgccaacgtc
     7561 tgaaaagatg atggcagatg gtgactctat ggacaaggtg tctagtgatt cgatgaaaga
     7621 cgggatggaa aaagaatcca tgatggacaa atcattctcg ggaacttttg ttggggtgga
     7681 cgcagtccat gatgcaaaag ggatggccaa gataataacg ctagatgacg gaagcgatgt
     7741 gctaagattg gaggatttta gatccacaaa tgggccagac ctgtatgtct atcttgcaac
     7801 agatgagact gcctctgatt ttgttaacct tggcaggcta aaggcaaaca ttggaaacca
     7861 gaactacaac atccctgaag gcacggatct atcaaagtat gactctgttc tggtatggtg
     7921 taagcagttc tcagttctgt ttggaaaagc agagctaagt tcataacaca attttttccc
     7981 tttatttttt ttatctagat t
//
LOCUS       DATIDL010000033        32122 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrosopumilaceae archaeon isolate SMAG_U16342
            k141_2266059, whole genome shotgun sequence.
ACCESSION   DATIDL010000033 DATIDL010000000
VERSION     DATIDL010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935836
            Sequence Read Archive: SRR25158326
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosopumilaceae archaeon (soil metagenome)
  ORGANISM  Nitrosopumilaceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrosopumilales;
            Nitrosopumilaceae.
REFERENCE   1  (bases 1 to 32122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32122)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/04/2024 05:09:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,520
            CDSs (total)                 :: 1,474
            Genes (coding)               :: 1,474
            CDSs (with protein)          :: 1,474
            Genes (RNA)                  :: 46
            rRNAs                        :: 1 (5S)
            complete rRNAs               :: 1 (5S)
            tRNAs                        :: 43
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32122
                     /organism="Nitrosopumilaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2266059"
                     /isolate="SMAG_U16342"
                     /isolation_source="soil"
                     /db_xref="taxon:2202732"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..130)
                     /locus_tag="VLB45_01400"
     CDS             complement(<1..130)
                     /locus_tag="VLB45_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-demethylwyosine synthase TYW1"
                     /protein_id="HSD04394.1"
                     /translation="MSCSGETVESDNEIIQIKSTISNQLKKAKYGISDHSTVELCHW"
     gene            215..1285
                     /locus_tag="VLB45_01405"
     CDS             215..1285
                     /locus_tag="VLB45_01405"
                     /inference="COORDINATES: protein motif:HMM:NF013694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HSD04395.1"
                     /translation="MKTSFYRMIKAVSVAGLEKEGIPFKKPSVQLSEAAQTVSLGNLT
                     WIECVVDNIVEETPKILENLGITMDPSVLLSGYVSNYEDRGETLGLMLPFVITGANKT
                     QTSPLLIFVKKDLVVTIHDDYGGKITRLYNYSSTLLRKLPKEPENWAERQTILVARII
                     DELSETNFSILRLIVERAEQLELDLAGARQIQKDLSLELSDMKTSLLTFLNAVWATYD
                     TVQNLKNGDADMISDQDVVLAKFEVLLGRLDRQIQMSENIMQVVATGINVIQAEVSNK
                     LAALIVWFTVAGTAVLVPNTLATIFGLYPNTEENLSWMLPTLGIATAISVFVTYKYTK
                     RWRVKSFSMKRLRSIKKKFRYN"
     gene            1525..1596
                     /locus_tag="VLB45_01410"
     tRNA            1525..1596
                     /locus_tag="VLB45_01410"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1558..1560,aa:His,seq:gtg)
     gene            complement(1606..1953)
                     /locus_tag="VLB45_01415"
     CDS             complement(1606..1953)
                     /locus_tag="VLB45_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04396.1"
                     /translation="MDERININVSAIDYENTSKEIVSTLRSLEQMVHGENEFVVTDSE
                     FAFGWHFYVVCVNKSLVQKLSDQMGPSFDEIKGKGLEKKFLTWLTDRLAQKNLKIKLA
                     IKEEMESSKYGIF"
     gene            complement(2003..2776)
                     /locus_tag="VLB45_01420"
     CDS             complement(2003..2776)
                     /locus_tag="VLB45_01420"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HSD04397.1"
                     /translation="MKIEDYLASLPQSIISGQDVQLPDNAFREMFQFAKLNKNDVFYH
                     LGCGDGKGIAIALEEFGVQKAVGIDNDMQKITLAKKLVDEKNLSNAVLQCSDIIESDF
                     SNATVILFWFSDQRVIESMTRKFSLLKKGCKIITIWSPLPETIPEKVDFPYILNVVPF
                     RKAKDLKEQILAVFGTDCIDFVTAWEFAERYTKAIGSTEAGNDRFLTIMQSLVIWINA
                     KNLGIACGNEIPQPIKTYMSILKTFFNIDVEYLLNQHQS"
     gene            complement(2802..3074)
                     /locus_tag="VLB45_01425"
     CDS             complement(2802..3074)
                     /locus_tag="VLB45_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0147 family protein"
                     /protein_id="HSD04398.1"
                     /translation="MVDNKSQNTETLNQAITTLDEIASNPSTPKNIKKSISDLATELK
                     KQEYPVSVRAANAISLLDDITQDPNMPSYVRVTLWQAVSTLERIRE"
     gene            complement(3123..3431)
                     /locus_tag="VLB45_01430"
     CDS             complement(3123..3431)
                     /locus_tag="VLB45_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme iron oxygenase ferredoxin subunit"
                     /protein_id="HSD04399.1"
                     /translation="MGKIIVGKTSDIPAGKMLKIIADGKEILVTNVDGNYFAMDDTCT
                     HAGASLADGKLDGSIITCDWHGAKFDCKTGKLSAFPAKIKDLNSYKVVVESDNVFLEI
                     "
     gene            complement(3474..4337)
                     /locus_tag="VLB45_01435"
     CDS             complement(3474..4337)
                     /locus_tag="VLB45_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HSD04400.1"
                     /translation="MKIARLLGSDMETYGFVSGDNVVTKEEITHQTGIPIPQNVKDFL
                     FDGWYEEIKQRESKLKYTQKISNFKFLAPIPNPSKIICLAFNYKDHAMEQNLIPPDEP
                     AIVIKPKTTLNGANSAIVCPSFVSKLDYEIELALVIGKDCKNIKEQDAKDAIFGYMIL
                     NDASARDIQAKDKQFTRAKGFDTFAPCGPWITTSDEISDPQNLKMLTKINGIVRQNSS
                     TSNMFLKIPSIVSMLSKVMTLEKGDIISTGTPAGVMLNKPDAVYLKDGDKIEMEIEGL
                     GKLENIVKFAN"
     gene            complement(4344..6050)
                     /gene="gltX"
                     /locus_tag="VLB45_01440"
     CDS             complement(4344..6050)
                     /gene="gltX"
                     /locus_tag="VLB45_01440"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HSD04401.1"
                     /translation="MDDELRKAIRGIALQNASEHEGKTRNDAVISKILGLRSDLRSKA
                     KEIMPLISEIVSEVNRYSLAEQKSELESSFPELLTTKPKQERIGLPPLEGAENGKVVT
                     RFPPEPNGYPHIGHAKAAIIDEEYARMYDGKLILRFDDTNPENERLEYYAAIKVGLDW
                     LGVKYNHVKNTSDDIELIYKKGLQMVETNNAYVCTCNQETISKNRREMKSCKCRSGDL
                     NQNTKRWHDMFEKYKPGEAIVRFRGDMESENTVMRDPALFRIIEGNHPLLADKYRVWP
                     NYDFAVSIEDSIDGITHAFRTKEYELRNELYYAILDKLQMRKPKMLEFSRLEFEGMPV
                     SKRILKPLVENCQVSGYDDPRLPTLEAMRRRGIVPEAIRKFVLSLSFTKSDTLAPFDT
                     LESFNRKIVDSSSIRLFMVIEPVKLTVKNNKLSVVELPNHPQKDIGKRRVEIDGIFYV
                     ASNDIKNLKVGDQLRLIELYNIKITKIGSDIEAEYAGDDFKAELPKIQWIPQKSATKI
                     NILIPKMLFVNEKFNENSLETLQAFTESHYLELNEGAEIQFVRFGYCRKDSAIQAIYT
                     HK"
     gene            complement(6051..7049)
                     /locus_tag="VLB45_01445"
     CDS             complement(6051..7049)
                     /locus_tag="VLB45_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HSD04402.1"
                     /translation="MGAEKIVSANSISKTARIVNSFLLSKLAGNPAQLYKAASHLIEH
                     GGKRLRPYMVVKSCEILGGNMKQALAAAASVEMIHNFTLVHDDIMDNDEMRHGVPTVH
                     MKFGIPVGILAGDVLFSKAFEIISSESKMPSKVSTALVSSLSKACVDVCEGQILDIMM
                     AQSQKIPTENQYVTMIEKKTSALFEVSCAMGAICADKKRKDVSNLSSFGKNLGIAFQI
                     VDDLIGIIGDSKITKKPVGNDIREGKKSLPILLAINSANKQDKQIILDAFGNTSVSKN
                     EIEVVVRTIRSMGIEKKVRKKAMQYSNAARKSLSEYDGSAKKELVSLLEFVVERSL"
     gene            complement(7031..7666)
                     /gene="idi"
                     /locus_tag="VLB45_01450"
     CDS             complement(7031..7666)
                     /gene="idi"
                     /locus_tag="VLB45_01450"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007549685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HSD04403.1"
                     /translation="MEYLILVDKNDNPIGTEEKVKCHLPNGKLHRAFTILLFNVEGKL
                     LLTRRSQSKMLWPGDWDGTVASHPRQSETYVSSAERRLPEEIGISCKLDYLFKFEYHV
                     PYKNIGSENEICGTLIGIVKDPSKIKLVKEEIDAIKWVSFDEILDDIKKSPQTYCPWM
                     ILALYFLPESETDILPEHRSILNGWIRSDIKQVFEKSLQHHFPDKKWELKK"
     gene            complement(7677..8417)
                     /locus_tag="VLB45_01455"
     CDS             complement(7677..8417)
                     /locus_tag="VLB45_01455"
                     /EC_number="2.7.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl phosphate kinase"
                     /protein_id="HSD04404.1"
                     /translation="MILIKLGGSIITNKQKPLTPNLLAINKIASKLKMVKEPIIVVHG
                     GGSFGHYWSVKYDMHTKPAKYNKKGVVVVKNSMVDLNKIILNSFLKHGLKPYCLPPAD
                     FMFGNKPIAKKIREISKIAKAGFIPVSYGDVMWHGKNKFYILSGDKIIGILARILKPR
                     LVIFVLNVDGVYSNMKSKKLLREIKGKKATISKVGMDVTGGMSRKIVEATKISKGGID
                     VFLVNGNMPTRITDAIKNRKFEGTIFRR"
     gene            complement(8443..9399)
                     /gene="mvk"
                     /locus_tag="VLB45_01460"
     CDS             complement(8443..9399)
                     /gene="mvk"
                     /locus_tag="VLB45_01460"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004496 - mevalonate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HSD04405.1"
                     /translation="MKSIASAPGKVILFGEHFVVYGTKAILCSIDKRIRVTSQIIEDK
                     VIRIRSGVGKAEADINLDSISDESKTEIMKPFFYIANQALRRFKKNTGVEIVIESEIP
                     PGIGLGSSSASCVAVSASVMGLFQKLSREEILKIAIDAERTMFENTSGADCSVCTFGG
                     LMQYDIKNRSSKINSKANFDLVIANSKHPHITSEVVSKVRQFKEKNGELFSSMYQQES
                     SIIQKALVALEKNDIQTLGLLMSENQMLLEKMGVSTDKLDLLINEAKKTAWGAKITGA
                     GGGGCIIALADKSNLDETLMNLKKYGDSFSAKIDYKGLLDTF"
     gene            complement(9396..10232)
                     /locus_tag="VLB45_01465"
     CDS             complement(9396..10232)
                     /locus_tag="VLB45_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010192025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MEMO1 family protein"
                     /protein_id="HSD04406.1"
                     /translation="MQIRTPAVAGMFYPKEKNELKTIIRNCFLHPFGSGKIPPNNDNQ
                     NVLGVICPHAGYMYSGPVATNSFYSISSQKPELVIIIGPNHWGIGCNIAVMKEGVWQT
                     PLGDVEVDKESAIKINEISKHIELDFFSHTKDHSLEVQIPMLQEIYSHKFKILPIILI
                     NQDYESAKKVGSAMAKIAKMKKTIIIGSSDFTHYEENSYAHKQDKSLIEPILDLDLDR
                     FYSVLQEKQVSACGYGAIASTMIACKELGATKGNLLRYATSGDVTGEKNSVVGYAAIT
                     FS"
     gene            complement(10237..10905)
                     /gene="rpsB"
                     /locus_tag="VLB45_01470"
     CDS             complement(10237..10905)
                     /gene="rpsB"
                     /locus_tag="VLB45_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HSD04407.1"
                     /translation="MSEAGDLENIEKSILSTGIRVGTPVKTKFMTSFITKATPEGLYI
                     IDIKKTLSRIQTAAKFINRAEIDKVIVCSGREYATTPIEKFCEVTGATKMLGRFMPGT
                     LTNPLLPYYIEPQLVIISDPQTDAQAVLEATNAGIPVIGLSNTDNVTSKIDLIIPANN
                     RGRKSLATTYWLLAREVLIQKGQLKDGGSMKYEIDDFETKITEEELEEEPVKNTRFDR
                     YQRQ"
     gene            complement(10915..12153)
                     /locus_tag="VLB45_01475"
     CDS             complement(10915..12153)
                     /locus_tag="VLB45_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase"
                     /protein_id="HSD04408.1"
                     /translation="MPKITSVKGRILYNSRGSKTIEIDVVSDNSHLGRVCAPSGASVG
                     KHEAVSFPENKPEKSLEILNANSKKFVGLDSSDLRTIHRTIREIDSTQDYSKIGGAVA
                     FALTISSVDSASRALGIPLFKLLTNEKELRFPFPLGNILGGGAHAGPGTPDIQEILIC
                     STGAKTIHDAIETNLVVHKELRKILEKHEPGFTNGRGDEGGWAPKLDNQKALEFSAKA
                     CESLGFTLGKEVSLGVDFASSTQWNEKKKKYVYNRAGFENTPEKQLEFVAEIIRKYKL
                     VYAEDAVHEEAFDEMAVLTSRFPNVLITGDDLVVTNAEILKKAAKVKACNAAILKVNQ
                     AGSLYDALEFASEAHKNNIKLITSHRSGESVDSHISHIGIATKSKMLKVGVVGGERIA
                     KLNELLRIAEYDLIRGMAQI"
     gene            complement(12155..12391)
                     /locus_tag="VLB45_01480"
     CDS             complement(12155..12391)
                     /locus_tag="VLB45_01480"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit N"
                     /protein_id="HSD04409.1"
                     /translation="MLIPVRCFTCGNLIADRFKDYQTRVKSGEDPAKVLDSLGFKRYC
                     CRTMMLTSVETIHQVIPFYEAIRRRQQEVQSELE"
     gene            12446..12530
                     /locus_tag="VLB45_01485"
     tRNA            12446..12530
                     /locus_tag="VLB45_01485"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12482..12484,aa:Leu,seq:gag)
     gene            12677..13327
                     /locus_tag="VLB45_01490"
     CDS             12677..13327
                     /locus_tag="VLB45_01490"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HSD04410.1"
                     /translation="MSDSAMGKLLVTVKPEKTPLSAREYPVIIGTVTDEASKPIGNAK
                     VKLTFAREVITTTTDSNGIFRYESARPSSPGQYAINVVATKEGYGTGFGSSNYFVNTP
                     APVTYSKGTKGAPLTVGNYTVYLGKVTEWNLEDTCFVVFGDEYKRFLNTCDLYAMAPE
                     NFKKPTKMISTLAVMKHNDEYRLFPVSAYYDAVKRSENTKTQYVISTWNNYTATNQ"
     gene            complement(13339..13953)
                     /locus_tag="VLB45_01495"
     CDS             complement(13339..13953)
                     /locus_tag="VLB45_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HSD04411.1"
                     /translation="MTLKNVKASLSKISNSLNKTQNAREYLIKSTREVIILCSQSIIS
                     IHRSDIKTGKEKAERAKKLLDEIRKKANGELYKHIMMPEQELVEAFAFLSVVEKSDIP
                     SAESLGVRGESYILGLLDCIGELKRLVYDSIIIGKVDDAKKFFEVMENLYLFLYPFAT
                     YDKIVNEARRKLDVNRILIEDTRAAVTEEIRRSVLIDAINKSKK"
     gene            14027..14677
                     /locus_tag="VLB45_01500"
     CDS             14027..14677
                     /locus_tag="VLB45_01500"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate epimerase"
                     /protein_id="HSD04412.1"
                     /translation="MDITVKQMMQIEENGHQLGFLRQFMMENAGAAAARYLLERFSDI
                     SSKKILVFAGLGNNGGDAFVVSRHLAGHGCHVKVVLLGNPSKIKTSESKTNWNLLEKM
                     ISLELIITSDARQLDVNADIIVDGILGTGISGNIKEPYASAIDMINKSDAFKLAVDVP
                     SGLDPDTGNTSDKCVKANATVTFHKMKVGMPKRKDLCGDIVVEKIGIPPEAEFGVL"
     gene            14674..15288
                     /locus_tag="VLB45_01505"
     CDS             14674..15288
                     /locus_tag="VLB45_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HSD04413.1"
                     /translation="MKVHQLEVGEMQNFTYILEDEETHDAVVIDPSWDLQDVLQIIEK
                     NNLKVNYIINTHYHFDHTIGNEFIVKATGAKIIQHKSSTLKNDMTVSNGDKIKFGNLE
                     LAVFHTPGHSKDSMCLVGDGKIFSGDTLFVGNCGRVDLPGGDARELYHSLFDIVSKMD
                     EKLVLYPGHNYGNSVTSTIGKEKKMNFVLQPRTEEEFVEFMNSE"
     gene            15285..16352
                     /locus_tag="VLB45_01510"
     CDS             15285..16352
                     /locus_tag="VLB45_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010192015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00303 family protein"
                     /protein_id="HSD04414.1"
                     /translation="MTNTNIDILGNVNKGNNFIKKTQNKKFVFSFVISYTETCEIPGI
                     TIAGANPELLKFTPAADAEFLHYGYCKSIDAIPMTPDGKPTPALLTKVALESASIPSF
                     VVDAGSKIAPMLPCIDLGLVPGKNIALEPALEPELIRKATEYGRIIGRSLGSLTDCLV
                     IGESIPGGTTTALGVLTGFGLNASVSSSMSQNPTELKNQTVQTALKRLQSRDPFDIVS
                     QLGDPMIPSVAGMLSAASESTNVILAGGTQMAAVLAFAKSIGFNEENTAIATTSYIIE
                     DNTANLVDVVRKISDIPILVAKLQLQQSKFIGLRSYAEGFVKEGAGAGGASVACMIKT
                     GLDSRTLLSLTEKEYQRITLQ"
     gene            complement(16339..18099)
                     /gene="glmS"
                     /locus_tag="VLB45_01515"
     CDS             complement(16339..18099)
                     /gene="glmS"
                     /locus_tag="VLB45_01515"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HSD04415.1"
                     /translation="MCSIIGYLGEGLAAPVLVKGLKRMEYRGYDSVGVATFSENQISV
                     RKGVGKVSEVNNTVKLDTLSGSVGIGHTRWATHGGVTETNAHPHPSSSGEVAIVHNGI
                     IENYAELKKDLQGKGYSFKSETDSEAIANLLQYNFDQIKDIKKAMMQTVSELKGNYAF
                     VAVFGDGTLSAARFHEPLIIGVGKEGYFVSSDVLGFVEHTDEVIYPDNREFVVINPSG
                     IQIFDFDGNPVQHQITKISKELADVYKGDYAHFTLKEISEQPDTVLKSGNNTKIELDL
                     ATEFVKHAKNLYITGSGTSYNSALIAKHLFSKYAKIKAEPIISSEVQFSPNYFDHQSI
                     LIAISQSGESADVLEAVKIAKKDGAKIISIVNIMTSSLVQDSSISIGLNCGPEIGVAA
                     TKSFTSQLGVLYKIADKLCEGCIGLDFNKVSESISKILSDHTKIQDIAKKLKVVSDIY
                     VLGRGIHYPIASEAALKLKELTYVHAEGIPGGELKHGPLALMDSNVYVLIINPVDSTY
                     SDTLASAHEIKARGAKIIGISDKPSDVYDYWIEIPSVNEALFPLVEIIPIQLLAYYSA
                     LEKDTDPDYPRNLAKSVTVK"
     gene            complement(18149..18685)
                     /locus_tag="VLB45_01520"
     CDS             complement(18149..18685)
                     /locus_tag="VLB45_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HSD04416.1"
                     /translation="MGDPKNSRKIWRKPKRPLNYDLLNEELYVLGTFGLKNKRELWKA
                     HTELSRVRNQARSLLALTQEVREKKEQVLMKSLIRIGLVNENSTLDDVLNLKVTDLLS
                     RRLQTIVQKKGLIKSPYQARQAVVHGHVMIGDRVITVPSYTVKTEEESQIHLAAGVNV
                     SQPKQQMVEATVAEQPQE"
     gene            complement(18695..19321)
                     /locus_tag="VLB45_01525"
     CDS             complement(18695..19321)
                     /locus_tag="VLB45_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HSD04417.1"
                     /translation="MASQEYRHIVRIAGKDVPGAKKMIIGVSQVRGIGYNFAKAILDV
                     LKIDQNSNVGFLSESQVEAIEKAMKNPSSLNIPSWFLNRRKDIDSGNDLHLITSDIEF
                     NVRNDIEREKNTNSWRGFRHTYGLKVRGQRTRTTGRKGGAVGVRKGGKVLPPGAVPAE
                     GVAAPAEGAAAPAATTAPAAGAKTAAPAAATKDAKPAPAKPTEKKPTK"
     gene            19405..21039
                     /locus_tag="VLB45_01530"
     CDS             19405..21039
                     /locus_tag="VLB45_01530"
                     /inference="COORDINATES: protein motif:HMM:NF019348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HSD04418.1"
                     /translation="MGQEISELVKRSYSSVPKYTEIMAGFPEDHEQIKTLGDLLEINY
                     KIVGAKEQLRQNLISKIQTNSPKYPGIIGFDDDVIPALDRAILSCHDVMLVGQIGQAK
                     TKIAETIAENLLSPIPVIKGSITNDSPVDLPADELISLLEDQDYVMATPKFYIDSTSI
                     EKIRNNKLDTQIEWVEGKCRFKYVLATPDISVKDLVGQIDAIKITKKGIEMYQIESYS
                     PGQLMQAKHGIFCIDELPVLDTRKQVALLSVLQEGRFTTGAYPVIFEPKTAFFATANP
                     IDYTHSGKIIEPLYDRLRSHIHTHYPKSIEDEMIIIIQEAKISKSFVPIFMLKILARI
                     IQNARTSQEINQDKGVSVRMGVHCLELLIGEAERTRSILHNIMAIPRPCDIFCISQAA
                     KFELTEMDDTVANREKVLRDLIMSSLKEYSLEYIKDIDPVLLEKIKQEFVGKTFQVSQ
                     KLVGMNSIEASYENQLRGFETMKNLIHKIFERVREEHREFEEKTHKYGINSELLLLSD
                     QTSNELRAAITEIVLEGLCWIEPKILDKKEDGYVAT"
     gene            21026..22378
                     /locus_tag="VLB45_01535"
     CDS             21026..22378
                     /locus_tag="VLB45_01535"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HSD04419.1"
                     /translation="MSQPDAFSLLYFLNQEKKDKKSNDEISDDTLQKILQTLAKQALK
                     EKTMNMQDLERILQESISQPNEQQPQEKNYENTETEIHGSGEPITKYLFKKGYLKDEK
                     KWLTNKGFFEIGGKILTDVMKTINEGGSGLHETKKIGSGNVIQDTSKKFELGDDLKFL
                     NVPQSVLNSVQRIAKKNSEIKFPIEMKLEDFEEFETTEEVKVAVVYCIDLSSTMKYSI
                     GAGEGSRIEAAKKALWSLYVLNKKFFPNDSIYVVGFGSFASEVNPHDIPYLKTYDAND
                     NFLHYTNYQAALRLALKILKQDGAKNKRIVVITDGQPSACFIDNENQKNDILAEKPYS
                     HFYKPDDATLLKIQNERELKLDTGNGQLVYLCYRYRQVDPFIDSKTLSEAKKCKREGI
                     EIDTIMVSEEDELLTYVEGLEMQLKGRAYYINPGNIDHVLINDFISNKKKVLNSKQTW
                     "
     gene            22384..22695
                     /locus_tag="VLB45_01540"
     CDS             22384..22695
                     /locus_tag="VLB45_01540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04420.1"
                     /translation="MSENYDKQKMLETLTDPEVSAILLELEDKSKELSFLTDKLQMTE
                     NEIYEKLSYVIQYGFVHIQKNENSVIFVVDQEKLNKIMESDENFSGVVDGLTKLDSFL
                     N"
     gene            complement(22688..23200)
                     /locus_tag="VLB45_01545"
     CDS             complement(22688..23200)
                     /locus_tag="VLB45_01545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04421.1"
                     /translation="MKKPLLLLVIGGFLVVFGIGLSVYGSQLIIENLATQDQRLGIGM
                     SMEITKDLDLSTNENGVYVIQITDFKNEDRVDATVFDPSGQILTSKTIEHSPLQDNFE
                     ILTSGTFKLLVENLSEREIDVTAVIGNLPQDESLTISIFGFIVIIIGLVGLVVGIIYF
                     IKSRGKTDVN"
     gene            23348..24382
                     /locus_tag="VLB45_01550"
     CDS             23348..24382
                     /locus_tag="VLB45_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008300970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HSD04422.1"
                     /translation="MFLALIIACVISVIYFVLPVFDIAAENEPKTFLTHSGALIKTTG
                     DVLDPIGYSGMPLEFNPDKYLREFSYGRTSSLSDGTVVREFTIIAEDDKIMEISPGVF
                     YNVWTFNGTVPGPTIRATEGDLVRIHFINNGKKSHTMHFHGIHEAEMDGVLEPVGQNG
                     GRFTYEFVAGPVGIHPYHCHVMPLEEHISHGLYGAYIVDPKEGRSTADEMVMVLNGFD
                     TDFDTENNFYAANTIPFYYQHHPIQINTNELIRIYVVNILEFDQINNLHLHGNLFDYY
                     PTGTYRIPSAYTDVITFSQGERGIMEFSYTYPGKYLFHAHKTEFAEKGWTGLFLVKNS
                     TENKNLYGDQ"
     gene            24382..25557
                     /locus_tag="VLB45_01555"
     CDS             24382..25557
                     /locus_tag="VLB45_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation transporter"
                     /protein_id="HSD04423.1"
                     /translation="MEEAKKTSKAKVVVTAIIPIVILAGMIAYLFGPGSNLLDFGVSL
                     PEITIEKVSFHDSKIVITIRNTGPIEVDIVQADVNDRIQPAAIEPDKHLGRFETAQVI
                     IPFEWNEAEPYEIGITVNDGTRFAKSVTAAALSPEPNFELASYFAVIGIYVGIIPVLI
                     GLLWFPFIAKMSKSKYKFFLALTAGLLLFLGIDAIVEGLKISTENLADSFNGPLLVAT
                     VVVISFLGLYYSSEKLIGRVTSSANKPVVIALMIAIGIGLHNLGEGLAIGAAISLGEI
                     ALSTFLIVGFTIHNTTEGLAIAAPMARGKPMIGKLAAMGMIAGTPAIFGAWMGGFIYS
                     PFAAVVFLSIGAGAIFQVVISLVNWMRQDGEKIFSTASVMSGFAIGMLVMYLTSIFV"
     gene            25661..28657
                     /locus_tag="VLB45_01560"
     CDS             25661..28657
                     /locus_tag="VLB45_01560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HSD04424.1"
                     /translation="MLLILFIIASFSIISVEAQSSHPNLFVSAENNTFENHFFGSMVI
                     EVIIRDNNISDTDSGIGEPDVTLNGNKLRMVQGSDGNWYAYFANLQKAKAADQIVPLA
                     VASKSLDFGVFCAESTDASVLGASFSGTEGVAIPDDNGITGATQGTSSFTACTGVLGT
                     PSNQNNVIRSSRSINLNPSVSPGQIGINPNAWPIVQLFSFNDNVVIIYNKGGGAQKVE
                     LVYSDIPNISFNLDRNSYPKGSEVFATINDMQLNQDPTSVDSWTFNVNSTRATFYQAF
                     TESGSNSGNGGLGLVNLNPYLASLDFEKNGNVGLNLGSVVQLRTNQFQPTSSVTTGTT
                     VYTKIVTFVETEPNSGIFESFDFNDESTIGISNNAPRGQSDSIEYNSKSKSIISGSFT
                     ASLSFVTQSGKFNPGQKATITLTDNDQNINPGTREHLDVFRSSAIIPTLQIGNPVTLE
                     KTSSVIFYPQSSSFVGGTSFSATVPDKNSDRLVIPATASPDLSFEKITLDLGMTTNTL
                     HNLFIDVPTQSGTNWINYDLRSFEQQLGITSFSDTKMVLYFGSLGSSPVQILGAGQIS
                     SGKGFVQISDSVIDSINVLPSSSSVFLEMDFDASNNNIGVGTISAETDTQPIVFDLFS
                     FGERNDNQVNNAIYRAELEETSANSGTFTGTIEYAVTNQLNQFDPAFITSLRTIDDEI
                     KFLINDELDDAQGTNLAYSDIANVGVTIGISSKTDIKTHAGTVTLESQTYRFGQPVVV
                     VLTDPDLNLKHDVVDVYSVVNDPSSDADDTVGDTSGNTLLEIEIKGFRYHRCTINGIE
                     TGGLGSTGFTLVETGPATGIFKGAFKMPSQICNESRTKLISPAGGSIEVNYFDFRDDS
                     GQPREIGLTFLKPSSKPQNQFVVEGEPAPNIDIKPVQMKDSFDRPILKPVIGQKINFV
                     TEILNNDEKSQRYTYIIQVKDENNKVVDLRWVEGYADPAKKKTAGILWEVTSSGNYTV
                     EIFLWDGIDSALPLDKKTEYQLNVGS"
     gene            complement(28698..29855)
                     /locus_tag="VLB45_01565"
     CDS             complement(28698..29855)
                     /locus_tag="VLB45_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010191982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HSD04425.1"
                     /translation="MIYLDNAASTPVHERAVEEMMPYFRAQYGNPSSIHKYGRLASVA
                     IQNARKRIASLINADPKEVLITSGGTESNNTVLYGMARKNKGKHIITSSIEHDAILEP
                     CKKLESEGFHVTYLPVDRYGFVNLEVLESEISSDTCLVSIMFANNEVGTVQPIKEIAK
                     IVHEKNIPFHTDAVQAVGKTPVDVKELGIDLMSISSHKINGPKGVGALYVKNGIKIDP
                     IILGGGQENGFRSGTENVAGVVGFGKACQLAKDNMEQNLEHFKKLGNMLCSQIIKEIP
                     HVTVNGHLEKRIPNNTHFTFLGVNGEDLIIKLDENGIAASTGSACSVRVQKASHVLKA
                     MGFSHEQIMGSLRLTIGISNTKEEIDETVRILKKVVKELRTVSPYKEKYNF"
     gene            complement(29852..30652)
                     /gene="larE"
                     /locus_tag="VLB45_01570"
     CDS             complement(29852..30652)
                     /gene="larE"
                     /locus_tag="VLB45_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010191984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HSD04426.1"
                     /translation="MKKIDELTHWFDDKKNALVALSGGVDSALVAYAAYRSLGTSSVA
                     VTADYKTLAQDELESAKKISVEIGIKHIIIEYNELKNPDFVKNDKNRCFHCRMELSEH
                     LKQIANKEKIDLIVDGTNLDDLGDYRPGIKALKENGIKSPLVETGFTKDEIRKEARRI
                     GLSVYDRPSNSCLASRIPWGQQVTVEKLARIEIGETTVKQLFGIRQVRIRDLDGNARI
                     EVMPEEIKLLSDKIKLIELDSRLKQIGFRTITIDPDGYRPGKLNVIAD"
     gene            complement(30649..31881)
                     /gene="larC"
                     /locus_tag="VLB45_01575"
     CDS             complement(30649..31881)
                     /gene="larC"
                     /locus_tag="VLB45_01575"
                     /EC_number="4.99.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HSD04427.1"
                     /translation="MVVVIDSQVAGISGDMLLSALIDIGAKKSKVIDGIHVAENYLTG
                     SRIQKIDFGSVTKHGTKATELLLDIREDTYERKGIEIQECILLTSDKIGLSEKSKVFA
                     KESIKSLINAESKIHGEPVESVHFHEASSIDTVVDIIGSAIALDDLDFFSNEIVSTPV
                     AIGGGMLNFSHGTVSNPASAILEIFMGTDIIISGGQVKEEITTPTGASLLVNLADRCS
                     EFYPSMKVRSIGYGAGKKNFDGFANVLKIVRGENTNQFQQDTVQILETNLDDVSGEVL
                     GHMIDKVISNGAKDVTVAPAITKKGRPTHLVSIICDSNTVNNILDTLVSETGTLGVRV
                     RNSARYIVPRTIVTVPVTIHGKRFSVKCKIVNREGTAKHFKVESADIKTIAESMSLSF
                     KDAYDIIRSQIKDVIDTK"
BASE COUNT         9779 a         6062 c         5548 g        10733 t
ORIGIN      
        1 tccaatggca tagctctact gtagaatgat cagagatccc atactttgct ttctttaatt
       61 gatttgatat cgttgatttt atttggatta tttcattatc tgattcaacc gtctcgccag
      121 aacaactcat gggcattaat ctgatatggt tttaattaaa tctacaggaa tttgtaaaat
      181 cagtaagagt tgattcacaa ataggtgtat ataagtgaaa actagttttt accgcatgat
      241 aaaggctgta agtgttgcag gtttagagaa agaaggcatc ccattcaaaa aaccaagcgt
      301 tcaactttca gaagctgcac agaccgtatc tcttggaaat cttacctgga ttgaatgcgt
      361 tgtagataac attgtagaag aaaccccaaa aattcttgaa aacctcggta taaccatgga
      421 tccatcagtt ttgctttctg ggtatgtatc aaattatgaa gacagaggag aaactcttgg
      481 tctgatgctt ccatttgtga tcactggcgc aaataaaacc caaacctcac cacttcttat
      541 atttgtcaaa aaagatcttg tagttacaat tcatgatgac tatgggggaa aaataacgcg
      601 cctgtacaat tattcaagca cgcttttacg caagcttcca aaagaaccag aaaattgggc
      661 tgaaagacaa accatacttg tagcacgaat aatagatgaa ctcagcgaaa caaacttttc
      721 cattttacgt ttgattgtag aaagggctga acaattagaa cttgatctag ctggtgcaag
      781 acaaatccaa aaagatcttt cgcttgaact ttcagacatg aaaacatcgc ttcttacatt
      841 ccttaatgca gtttgggcta catatgatac tgttcagaac ctcaagaatg gtgatgcgga
      901 tatgatatca gatcaggacg tcgttcttgc caagttcgaa gttcttcttg gaagactaga
      961 tcgtcagatc cagatgtcag aaaatatcat gcaagtagtt gctacaggaa ttaatgtcat
     1021 tcaagccgaa gtttcaaata aacttgcagc tctaatagtg tggtttactg tagctggaac
     1081 agctgtattg gtaccaaaca cacttgcaac tatttttggt ttgtatccaa acactgaaga
     1141 aaatcttagc tggatgctac ctactctggg catagctacc gcaatttctg tctttgttac
     1201 atacaagtac acaaaacgat ggagagtcaa atcatttagc atgaaacgac ttagaagcat
     1261 aaagaagaaa tttcgttata actagctgta ttgtgaaaaa ttcttagatg agtctacttc
     1321 catggtagaa ggtagattgc accataaagt gtgagtactg aagtaattcc catggcaaca
     1381 aaccacaata ttccatttct catgttctgc gatatttttt tcggttgtaa taaacctaca
     1441 gttgactatc aagtttgtaa aaacatggta atgtaactaa tcagttaaga ttaatactgg
     1501 acagaaaagg atccgtcttt ggtggccctt gtagtacagt ggccagtata ggggactgtg
     1561 gatccccgga caggggttcg attcccctca agggcctttt ttattctaaa atatgccgta
     1621 tttgcttgat tccatttctt ctttaatggc aagcttgatc ttcaaattct tttgagcaag
     1681 tctatctgta agccaggtca agaatttttt ttccaaccct ttacctttta tttcatcaaa
     1741 actaggaccc atctgatctg atagcttctg tactagggat ttgtttacac agacgacata
     1801 aaagtgccac ccaaatgcaa actctgaatc tgtaacaaca aattcatttt caccatgcac
     1861 catttgctct aaacttctta aagtagacac gatctcttta cttgtatttt cataatctat
     1921 ggcagatacg ttgatgttta ttctctcatc caacccactg taattaggtg gtttccagat
     1981 aaaagtttca ctgattatta gttcatgact gatgttgatt caaaaggtat tcgacatcta
     2041 tgttgaaaaa agtcttcaat atgctcatgt atgtcttgat gggttgaggt atttcgtttc
     2101 cacaggcaat gccaagattc tttgcattta tccatattac aagtgattgc attatagtaa
     2161 gaaagcgatc gtttccagct tcggtggaac ctatagcctt ggtatatctt tctgcaaatt
     2221 cccatgcagt gacaaaatct atacagtcag taccaaacac tgcaaggatc tgttctttta
     2281 gatcctttgc ctttctaaac ggaactacgt tgagaatgta aggaaagtct actttttctg
     2341 gaatagtttc tggtagagga ctccaaattg ttattatttt gcaccctttt tttaaaagag
     2401 aaaactttct agtcatgctt tcaattactc tctgatctga aaaccaaaac aaaataacag
     2461 ttgcattgct aaaatctgat tctattatgt cgctgcattg taaaaccgca tttgacagat
     2521 ttttttcatc aactaatttc ttagccaaag taattttttg catatcatta tctattccca
     2581 ctgctttttg cactccaaat tcttctaaag ctatggcaat acccttgcca tctccacacc
     2641 caagatgata gaaaacgtcg tttttgttta gtttggcaaa ttgaaacatt tctctaaatg
     2701 cattatctgg aagctgaaca tcttgtccag aaattattga ctgtggtaat gatgcaagat
     2761 agtcttctat tttcacaact gactaggtga ttgatctaaa tttattctct tattctttca
     2821 agcgtagata ctgcttgcca taatgtgact cttacatatg atggcatgtt tggatcttgc
     2881 gtgatgtcat caagcaggct aattgcgttt gcagctcgaa cagaaacagg gtattcttgt
     2941 ttttttagtt ctgttgctaa atctgatatg ctttttttga tgttttttgg agtagaaggg
     3001 tttgatgcta tttcatcaag tgtagtaatg gcttgattga gtgtttcagt gttctgagat
     3061 ttattatcga ccattctacc atcagagctt acctaattgc attgatttta taaagtttgc
     3121 acttaaatct ctagaaacac gttatctgat tcaacaacta ctttgtatga attcaaatct
     3181 ttgatttttg cgggaaacgc agacagctta ccagttttgc aatcaaattt tgcgccatgc
     3241 caatcacacg ttattatgga tccatcaagc tttccatctg caaggcttgc gcctgcatga
     3301 gtacatgtat catccattgc aaaataattt ccatcaacat tcgttacaag tatctctttt
     3361 ccatctgcaa taattttcaa catttttcca gctggaatgt ctgaagtttt gccaactata
     3421 attttgccca tagttttttt tataactagt taccttaata gttttttaag aaattaattt
     3481 gcaaatttga caatgttttc aagttttcca aggccttcta tctccatctc tatcttatcc
     3541 ccgtctttca aatatactgc atctggcttg tttaacatga ctcctgcagg agttccggtg
     3601 gatattatgt cgcctttttc taaggtcatc actttactta gcatggaaac aattgagggt
     3661 attttcaaaa acatgtttga agtagaagag ttttgtctga caattccatt tatctttgtc
     3721 agcattttga gattttgagg atcagatatt tcatctgatg tagtaatcca aggtccacat
     3781 ggtgcaaaag tatcaaatcc ttttgcacgt gtaaactgtt tgtcttttgc ttgtatgtct
     3841 cgtgctgaag catcatttaa gatcatgtat ccaaaaatag catcttttgc atcctgctct
     3901 ttgatgtttt tacaatcttt acctatgacc aaggcaagct ctatctcata atctagtttg
     3961 gacacaaagg acgggcatac tatggcggaa ttagccccgt tgagggttgt ttttggcttt
     4021 atcactatgg caggctcatc tgggggaatg agattttgtt ccattgcatg atccttgtag
     4081 ttaaaggcaa gacagattat tttagaaggg tttggaatag gcgcaagaaa tttaaaatta
     4141 gaaatttttt gtgtatattt taattttgat tctctttgtt ttatttcttc ataccatcca
     4201 tcaaaaagaa aatccttgac gttctgtggt atggggatac ctgtttggtg tgtgatctct
     4261 tcttttgtta ccacattatc tccactaaca aaaccatatg tttccatgtc tgatcctaac
     4321 aatcttgcaa ttttcatttt ttctcacttg tgagtataga ttgcctgaat tgcagagtct
     4381 tttctgcaat acccaaatct aacgaactga atttctgcac cctcatttaa ttctaagtag
     4441 tgactttcag taaaagcttg aagtgtttca agactgtttt cattaaattt ttcatttaca
     4501 aaaagcatct ttggtatcag aatgtttatc tttgtagcgc ttttttgtgg aatccattga
     4561 attttaggca gttctgcttt aaaatcatct ccagcatatt cggcctctat atcagaacca
     4621 atctttgtaa ttttaatatt ataaagctca atcaaccgta gctgatctcc gactttaaga
     4681 ttttttatat cgttgcttgc gacataaaaa attccatcaa tctcaactct tcttttgcca
     4741 atgtctttct gtggatggtt tggcagctca acaactgata gtttgttatt ttttactgtt
     4801 aattttacag gttctataac catgaacaat ctgatgctag aactgtctac tatctttctg
     4861 ttaaaagact ccaaggtatc aaatggagcc aacgtgtcag attttgtaaa actcaacgat
     4921 aagacaaatt ttcttattgc ttctggtaca attccgcgcc tgcgcattgc ttccagagtc
     4981 ggcagtcgtg gatcgtcata tcctgatact tggcagtttt caaccaatgg ttttagtatt
     5041 ctctttgaga caggcatccc ctcaaattct aatctggaaa attctagcat ctttggcttt
     5101 ctcatttgca gcttatccaa aatcgcatag tatagctcgt ttctaagctc gtattctttt
     5161 gttctaaatg catgagttat tccatcaata ctgtcttcta ttgatactgc aaaatcataa
     5221 tttggccata ctctgtactt gtctgcaagc agaggatgat tcccttctat tattctaaat
     5281 aatgcaggat ctcgcattac cgtattttct gattccatgt cgccacgaaa tctgactata
     5341 gcttcgccgg gcttgtattt ttcaaacatg tcgtgccatc tttttgtatt ttgatttaaa
     5401 tctccagaac gacacttaca ggacttcatt tctcttctgt ttttgctaat tgtttcttga
     5461 ttacatgtac aaacatatgc gttgtttgtt tcaaccatct gtaagccttt tttgtaaatt
     5521 aattcaatgt catctgatgt gtttttcaca tgattgtatt ttactcctaa ccaatctaat
     5581 ccaactttga tcgcagcata atactctaat cgctcatttt caggatttgt atcatcaaat
     5641 ctcaagatta gttttccatc atacatccga gcatattctt catcgattat ggctgccttt
     5701 gcatgaccaa tatgtggata tccatttggc tctggtggaa atctagttac cactttaccg
     5761 ttttctgcgc cttctaatgg tggtaaacca attctttcct gttttggttt tgtagtaagt
     5821 aactctggaa agctgctttc gagttctgat ttttgttcag caagagaata cctgttaact
     5881 tctgacacta tttctgatat gagtggcatt atttcctttg ccttgcttct aaggtcagat
     5941 cttaagccaa gtatttttga tatcactgca tcatttcgtg tttttccctc atgctcagaa
     6001 gcattctgta aggcaattcc tcttattgcc tttctcaact catcatccaa ctacaaactc
     6061 ctctctacca caaattctaa aagtgacaca agttcttttt ttgcagatcc gtcatactct
     6121 gacaatgatt ttcttgcggc attagagtac tgcattgctt tttttctaac tttcttctct
     6181 attcccattg atctaatggt tctaaccact acttcaattt cattttttga aactgaagta
     6241 tttccaaagg catccagtat tatttgctta tcttgtttgt ttgcactgtt tattgcaaga
     6301 agtattggga gagatttttt tccttctcta atatcatttc ccacaggttt ttttgtaatt
     6361 tttgaatcac ctatgatgcc aataagatca tcaactatct gaaatgcgat tcctagattc
     6421 tttccaaatg aagacaaatt tgatacatct tttctttttt tatctgcaca aattgctccc
     6481 attgcacatg atacttcaaa aagtgcggat gtctttttct ctatcatcgt gacatattga
     6541 ttttcagttg gtatcttttg tgactgtgcc atcattatgt caagtatctg tccttcgcat
     6601 acatcaacac aggctttaga taaactagaa acaagcgcgg tgcttacttt actgggcatt
     6661 ttagattctg atgaaattat ctcaaatgct tttgaaaaca aaacatctcc tgccaagatg
     6721 cctacaggaa tgccaaactt catgtgtacc gttggcactc catgacgcat ctcgtcatta
     6781 tccattatgt cgtcatggac aagagtaaag ttgtgtatca tctccactga agctgcagca
     6841 gctaatgcct gtttcatatt accgcccaaa atttcacagc tttttaccac catgtatgga
     6901 cgtaatctct ttccaccatg ttctatcaaa tgtgatgctg ctttgtagag ctgagctggg
     6961 ttgccagcaa gttttgagag taaaaatgag tttactattc tggctgtttt tgatatagag
     7021 tttgcagaca ctattttttc agctcccact ttttatctgg aaagtgatgc tgcaatgatt
     7081 tttcaaaaac ctgtttaata tctgatctaa tccatccatt taaaatcgac ctgtgttctg
     7141 gcaagatatc tgtttctgat tctggcaaaa aatacaacgc aagtatcatc catgggcaat
     7201 aagtctgtgg agatttttta atatcatcta aaatttcatc aaaagaaacc cattttatag
     7261 catcgatttc ttctttgact agtttaattt tggagggatc ttttactatc ccaattaaag
     7321 ttccacaaat ttcattttca gaacctatgt ttttgtatgg tacatgatat tcaaatttga
     7381 acaaataatc aagcttgcat gatatgccta tctcttctgg caatcttctt tctgcagacg
     7441 acacatatgt ttctgattgt ctgggatgac tagctacagt tccatcccaa tcacctggcc
     7501 aaagcatctt gctttgacta cgtcgcgtta aaaggagttt accttctaca ttaaacaaaa
     7561 gtattgtgaa tgctctatgg agcttgccgt taggtagatg gcatttgact ttttcctctg
     7621 ttccaatggg attgtcattc ttgtctacca gaataaggta ttccatgact caccatttat
     7681 ctcctaaata tcgtcccttc aaattttcta ttttttatcg catctgttat cctagttggc
     7741 atgttgccgt tcactagaaa tacatcaata ccgccctttg aaatttttgt ggcttccaca
     7801 attttgcgac tcatcccccc tgttacatcc attccaactt ttgatatggt ggcttttttg
     7861 cctttaattt cacgcaggag tttttttgat ttcatatttg aataaactcc atccacattt
     7921 aaaacaaaaa ttaccaatct aggctttaga attcttgcca gtattccaat gatcttgtca
     7981 cccgataaaa tatagaattt gtttttacca tgccacatca catctccata tgatacagga
     8041 ataaaacctg cttttgcaat ttttgaaatc tctcttattt tcttagcaat tggtttattg
     8101 ccaaacataa aatcagctgg aggaagacaa tacggtttta ggccatgttt taaaaatgaa
     8161 tttaaaatta ttttatttag atcgaccatg gaatttttta caactactac tccttttttg
     8221 ttgtattttg caggttttgt gtgcatatca tatttgacag accaataatg tccaaaagag
     8281 cctcctccat ggactacaat aattggctcc tttaccatct ttagtttaga cgcaattttg
     8341 tttatggcta ataggtttgg tgtaagaggt ttctgcttgt ttgtgattat tgaacctcca
     8401 agctttatca aaatcatatt tttttacagt aatccagata gtttaaaaag tatctaaaag
     8461 acctttgtag tcaattttgg cagagaagct gtctccatat ttctttagat tcatgagggt
     8521 ttcatcaaga tttgacttgt ctgcaagtgc tattatgcag ccacctcctc ctgcccctgt
     8581 gatctttgcc ccccacgcag tcttttttgc ctcatttatt agcagatcta gtttgtctgt
     8641 agaaacaccc attttttcta acaacatctg gttttcagac atgagaagac ccaatgtttg
     8701 aatatcattt ttttctagtg caacaagtgc tttttgaata attgatgatt cttgttgata
     8761 cattgatgaa aacaactctc catttttctc tttaaattgt cttaccttgc ttaccacttc
     8821 acttgtaata tggggatgtt tcgaatttgc aattaccaga tcaaaattag ctttggagtt
     8881 tattttacta gatcgatttt taatatcgta ttgcattagg cctccaaaag tacaaacaga
     8941 acaatctgcg ccagatgtat tttcaaacat ggttcgctca gcatctattg caatttttaa
     9001 aatttcttct cttgaaagtt tttggaataa tcccataact gatgccgata ctgctacaca
     9061 agaagcagaa gatgatccaa gtccaatgcc tggaggaatt tctgattcta ttacaatttc
     9121 taccccggtg ttttttttga atcggcgtaa ggcttgattt gcaatataaa aaaaaggttt
     9181 cattatttct gttttggatt catcagagat cgagtctaaa ttgatatctg cctctgcctt
     9241 tcctactcct gatcttattc ttattacctt gtcttctata atttgtgagg ttaccctgat
     9301 ccttttatct atggaacaaa gtattgcttt tgtcccataa acaacaaaat gctcaccaaa
     9361 taaaatcact ttaccaggcg cagatgcaat ggatttcatg aaaatgtaat cgccgcatat
     9421 cctaccacgg aattcttttc acctgtcacg tctccacttg tagcatatct caataggttt
     9481 ccctttgtgg ctcccagttc tttgcaggct atcatggtgg atgcaatcgc tccatatccg
     9541 cacgcactta cttgtttttc ttgtaaaaca ctatagaatc tatctaaatc taaatctaaa
     9601 atgggctcga tcaaagattt gtcctgttta tgcgcataac tattttcttc atagtgtgta
     9661 aagtcagatg aacctatgat gatagttttt ttcatctttg caatctttgc catggctgaa
     9721 ccaacttttt ttgctgattc atagtcttga tttatcaaaa ttatcggcaa aattttgaat
     9781 ttgtgggaat aaatttcttg caacatggga atttgtactt ctagactatg atcttttgtg
     9841 tgagaaaaaa aatctagttc tatatgttta gatatttcat ttatctttat ggccgattct
     9901 ttgtctactt caacatcgcc aagaggagtt tgccacactc cctccttcat cactgctatg
     9961 ttgcaaccaa tgccccaatg gtttggacca ataattatga caagttcagg tttttgtgat
    10021 gatatgctgt aaaaagaatt tgttgcaact ggaccagaat acatgtatcc ggcgtgcgga
    10081 caaataacac caagtacatt ttgattatca ttgtttggag ggattttgcc agatccaaaa
    10141 ggatgcaaaa agcagttgcg aatgatagtt tttaattcat ttttttcttt tggatagaac
    10201 ataccagcaa cggctggtgt tcttatctgc attattctat tgcctctggt atctatcaaa
    10261 acgtgtattt tttactggct cttcttctaa ttcttcttca gttatcttgg tttcaaaatc
    10321 gtctatttca tatttcattg atcctccgtc ttttagctga cctttctgta tgagtacttc
    10381 tcttgccaaa agccagtatg tagttgcaag agactttctt cctctgttgt ttgctggtat
    10441 gataagatca attttagaag tgacattgtc tgtatttgat aaacctatga ctggtatccc
    10501 agcgtttgta gcttcaagaa ctgcctgtgc atctgtttgc ggatctgaaa ttattacaag
    10561 ctgtggttct atgtaatatg gtaaaagtgg atttgttaga gttccgggca taaatcttcc
    10621 aagcattttt gtagctcctg ttacttcaca aaatttttct attggagtag ttgcatactc
    10681 tctccctgaa cacactatta ctttgtcaat ttctgctctg tttatgaatt tggccgcagt
    10741 ctgtattctg gaaagtgttt ttttaatatc tatgatgtaa agtccttctg gagttgcttt
    10801 tgtaataaat gaggtcatga attttgtttt aactggagtt cccactctta ttccagtgga
    10861 taggatgctt ttctctatat tttcaagatc tccggcttcg ctcatgttgt gtttctaaat
    10921 ttgtgccatt ccacgtatta aatcatactc tgcaatacga agcaattcgt tgagcttggc
    10981 aattcgttct cctccgacta ctcctacctt tagcatcttt gattttgttg caatgcctat
    11041 atgtgatatg tgagaatcca ccgattctcc agatctatgc gaagtgatta atttgatgtt
    11101 gtttttgtga gcctctgatg caaattctag tgcatcataa aggcttcccg cttgattgac
    11161 ttttagaatt gcggcgttac atgcctttac tttcgctgct tttttgagaa tctctgcatt
    11221 tgttaccact agatcatcac ccgttatcag gacatttgga aatcttgaag tcaaaactgc
    11281 catttcatca aaggcctctt catgaacagc atcttctgca taaacaagct tgtattttcg
    11341 tattatttct gcaacaaact ctaattgttt ttctggtgta ttttcaaacc ctgctctgtt
    11401 gtatacgtat ttctttttct tttcattcca ttgtgtagat gacgcaaaat ctactcctag
    11461 ggaaacttct ttacccaagg taaatccaag actctcacaa gcttttgcag aaaactctaa
    11521 tgctttctga ttgtctaatt ttggagccca acctccttcg tctcctcgtc catttgtaaa
    11581 tccaggttca tgtttttcca aaatctttcg taattcttta tggactacaa ggttagtttc
    11641 tattgcatca tgaattgtct ttgcgccagt tgaacaaatc agaatctctt gtatgtctgg
    11701 agttccggga cctgcatgtg cacctccacc taaaatattt ccaagtggaa aaggaaaccg
    11761 taattctttt tcatttgtta ataacttgaa taatggaatt cctagtgctc tagatgcaga
    11821 atcaacagac gatatggtaa gtgcaaaagc cactgctcct cctattttag aataatcctg
    11881 tgtagaatca atttcgcgta tagttctgtg tatggtcctt aaatcagaag aatcaaggcc
    11941 tacaaatttt tttgaatttg cattaagaat ttctaggctt ttctcaggtt tattttctgg
    12001 aaaactgact gcttcatgtt tgcccacgct tgccccagag ggagcacaaa ctcttccgag
    12061 atgtgaatta tcagaaacta cgtctatttc gattgtttta cttcctcgac tattgtagag
    12121 aatccttcct tttacggaag ttatctttgg catcctattc taactcagat tgtacttctt
    12181 gttgtcttct tctgattgct tcgtagaatg gaatcacttg atgaattgtt tcaacagatg
    12241 ttaacatcat agttctgcaa caatatcttt tgaatcctag agaatcaaga acttttgcag
    12301 gatcttctcc actttttact ctagtctgat aatctttaaa cctgtcagca attaaatttc
    12361 cacaagtaaa acatcggaca gggatcagca tattttgcaa aagtaatcaa ggcttataaa
    12421 aaccatgcat gggtatttgc ataatgcggg agtcgcccag cctggccaaa ggcgctagct
    12481 tgaggggcta gtatcttagg atttcgaggg ttcaaatccc ttctcccgca cttgatattt
    12541 tagccatcca taattgactc aactgataaa aacaatagat atcggatctt aacatacaaa
    12601 attaaaaccg cattatgtga aatctttttt catcggaata ataatttgca taattttgat
    12661 atctgctaat gtgcatgtgt cagattcagc aatgggaaag ctacttgtta cagtaaaacc
    12721 agaaaaaact ccgctttctg caagagaata cccagttatc attggaactg tgactgatga
    12781 agcaagtaag ccgataggaa atgccaaggt aaaattgaca tttgcaagag aagtgataac
    12841 tacaactaca gactctaatg gaatttttag atatgaatca gcaagaccct catctccagg
    12901 tcaatatgcg attaatgtgg ttgctacaaa ggaagggtat ggcacaggat ttggaagttc
    12961 aaattatttc gtaaacacac ctgccccagt tacgtatagt aaaggaacaa aaggcgcacc
    13021 cttgacagtt gggaattata ccgtatatct tggaaaagta acagagtgga atttagaaga
    13081 cacttgcttt gtagtatttg gggacgaata caaaagattc ctaaacacat gtgatctata
    13141 tgccatggca ccagaaaatt tcaaaaaacc taccaaaatg atctccacgt tagctgtaat
    13201 gaaacacaac gatgaatacc gattattccc agtatctgca tattatgatg ccgtaaagcg
    13261 atccgagaat accaaaactc aatatgtaat cagcacgtgg aacaattata ccgccacgaa
    13321 tcaataattg tattagacct attttttact cttatttatt gcgtcgataa gaacagatcg
    13381 tcttatttct tcggttacgg cagctctggt atcctcaata agaattctat ttacgtctag
    13441 ttttctgcgg gcttcgttta ctattttgtc atatgttgca aaaggataca aaaacagata
    13501 gagattttcc attacctcaa agaatttctt tgcgtcatcc acttttccta ttatgatact
    13561 gtcataaacc aatcttttta gctcaccaat acagtcaagc aaaccaagaa tgtatgattc
    13621 tcctctaact ccaagtgatt ctgcagaagg tatgtctgat ttttcaacca cagaaagaaa
    13681 cgcaaaagct tcaacaagtt cttgttccgg catcatgatg tgcttgtaaa gttcaccatt
    13741 tgcttttttt cgtatctcat cgagcagttt ttttgcacgc tcagcttttt ctttaccggt
    13801 ctttatgtca gatctatgaa tggatattat tgattgactg cacaaaatta tgacctcgcg
    13861 tgtactcttt atcaaatact cgcgagcgtt ttgagtttta tttagagaat ttgaaatttt
    13921 tgacaaagat gcctttacat tttttaatgt catgttggga taaataaaaa actaagataa
    13981 agccgtttgg taaagccctt atttgacatg ttttctgaaa tatcacgtgg atataacagt
    14041 aaaacagatg atgcagatag aagaaaatgg tcaccagtta ggttttctaa gacagtttat
    14101 gatggaaaat gcaggagcag ctgcggcaag atatttgtta gaaagatttt ctgatatttc
    14161 atcaaaaaag attctagttt ttgcaggtct tggaaataac ggaggcgatg catttgttgt
    14221 ctctaggcat ctggcaggac atggttgtca tgttaaagta gtattacttg gaaatccaag
    14281 taaaatcaag actagtgaaa gcaagacaaa ttggaatcta ctggaaaaga tgatctcact
    14341 tgaattgatt atcacctctg atgcaagaca attagatgta aatgcagaca ttattgtaga
    14401 tggaattttg ggaactggaa tatctggtaa cataaaggaa ccttatgcat ctgctataga
    14461 tatgataaac aaatccgatg cgttcaagct ggcagtagat gtgccttctg gtctagatcc
    14521 tgacactggt aatacaagcg ataaatgtgt aaaggcaaat gcaacagtga catttcataa
    14581 aatgaaagtt ggtatgccaa aaaggaagga tctgtgcggt gacattgtag tagaaaaaat
    14641 tggcattcca ccagaagccg agtttggtgt gttatgaaag ttcaccaact agaagtaggt
    14701 gagatgcaga actttactta catattggag gatgaagaaa cacatgatgc tgttgttata
    14761 gatccatctt gggatttaca agatgtactg caaataatag aaaaaaacaa tctcaaagta
    14821 aactacataa taaatacaca ttatcacttt gatcacacta ttggaaacga attcatagta
    14881 aaagccactg gtgcaaaaat aatacagcat aaatcttcta cgctaaaaaa tgacatgact
    14941 gtttctaacg gtgataagat caagtttgga aatctagaac ttgctgtttt tcacactcca
    15001 gggcactcaa aggacagcat gtgtctagtt ggggatggga aaattttttc aggtgataca
    15061 ctttttgtgg gtaactgtgg aagagttgat ctacctggtg gcgatgcaag ggaactttat
    15121 catagtcttt ttgatattgt aagtaaaatg gatgaaaaac tggttctata tcctggacat
    15181 aattatggca attcagtaac gtcaacaata ggtaaagaaa aaaagatgaa ctttgtactt
    15241 caaccaagga ctgaagaaga atttgtggaa ttcatgaata gtgaatgact aacacaaaca
    15301 tagacatctt aggaaatgtc aacaagggca ataattttat aaaaaaaaca caaaataaaa
    15361 aatttgtatt ctcatttgta atttcttaca ctgaaacatg tgaaattccg gggattacta
    15421 ttgctggcgc aaatccagaa ttactgaaat tcacacccgc ggcagatgct gaatttcttc
    15481 attacggtta ttgcaaaagc atagatgcca tacctatgac tcctgatggc aaaccaactc
    15541 ctgctctttt aactaaagtc gcactagaat ctgctagtat tccaagcttt gtagtagatg
    15601 ccggaagcaa aatagctcca atgcttccat gtatagatct tggattagta ccaggtaaaa
    15661 atatcgcact agaacctgct ttagaaccag aattaataag aaaagcaaca gaatatggac
    15721 gaatcattgg tagatcactt ggatctttaa ctgattgtct tgtaattggc gaaagcatac
    15781 ccggtggaac tacaactgca ttaggcgtgt tgacaggatt tggattaaat gcatctgtaa
    15841 gtagtagcat gtcacaaaat ccaactgaat taaaaaacca aacagtgcaa accgcactaa
    15901 aacgactaca atcacgagac ccatttgata ttgtttcaca gctcggtgat ccgatgattc
    15961 catctgtagc aggaatgcta agcgctgcgt ctgaatccac aaacgtgata ttggcaggag
    16021 gaactcagat ggctgcagtt ttggcttttg caaaaagtat tggttttaac gaagaaaata
    16081 ctgcaatagc aacaacgtca tacataatag aggacaacac tgcaaaccta gttgatgttg
    16141 taaggaaaat atctgatatt cctatacttg ttgcaaaact gcaacttcaa caatcaaaat
    16201 tcataggttt acgttcctat gctgaaggtt ttgttaagga aggtgctggt gctggaggcg
    16261 catcggtcgc atgtatgata aagacaggcc ttgacagcag aactcttctt tcattaactg
    16321 agaaagaata tcaacgaatt actttacagt aacagacttg gcaagatttc ttggataatc
    16381 aggatccgta tccttttcaa gtgcagaata gtatgccagt aattgaatag gtattatttc
    16441 aactagagga aacaatgctt catttactga tggaatttca atccaataat catacacatc
    16501 actaggctta tctgaaattc caattatctt tgcaccacga gctttgattt catgtgcact
    16561 tgctagtgtg tctgagtaag tcgaatctac tggatttatt atcaaaacat acacattgga
    16621 atccatcagt gctaaaggtc cgtgctttaa ctctcccccg ggaatacctt ctgcatgaac
    16681 atacgtgagt tcttttaatt tcaaagctgc ttcagacgca ataggataat gaatccctct
    16741 tccaagtaca tagatgtctg aaaccacttt gagttttttt gcgatatcct gaatcttggt
    16801 atgatcagaa agtatcttag atatggattc tgaaactttg ttgaaatcta atccaataca
    16861 tccttcacat agtttatctg ctatcttgta tagtactcct agctgtgacg tgaagctctt
    16921 ggtcgcagca accccgatct ctggtccaca attaagtccg atggaaatgg atgaatcctg
    16981 aacaagcgag gaagtcatta tgtttactat ggaaataatt tttgcgccat cttttttagc
    17041 aatttttact gcttcaagaa catctgcact ctcaccactt tgagagattg caattagtat
    17101 tgattgatga tcaaagtaat taggcgaaaa ctgtacctcg cttgatatta ttggttctgc
    17161 ttttatcttt gcatattttg agaaaagatg tttggcaata agagctgaat tataactagt
    17221 accgcttcca gtaatgtaaa gattttttgc atgctttaca aactctgtgg ccaagtcaag
    17281 ttcaatcttt gtattgtttc cagatttcaa tactgtatct ggctgttccg aaatttcttt
    17341 aagcgtaaaa tgcgcataat ctcctttgta cacatcagca agctcttttg atatttttgt
    17401 aatctgatgc tgtactgggt ttccatcaaa atcaaagatc tgaatgcctg atggattgat
    17461 caccacaaat tcacgattgt ctggatagat cacttcatct gtatgctcta caaatcctag
    17521 cacatcactt gaaacgaaat atccttcttt tcctactcct attatcaatg gttcatgaaa
    17581 tctagctgca gaaagtgttc catctccaaa cactgctaca aaagcataat ttccttttag
    17641 ctctgatact gtctgcatca ttgctttctt gatatctttt atctgatcaa agttgtattg
    17701 taaaagattt gcaatagcct cactatctgt ttcacttttg aaactatacc ctttcccttg
    17761 tagatctttt tttaattcag cataattctc aattatgcca ttatgtacta tggctacttc
    17821 gcctgaacta gatggatgtg gatgtgcatt tgtctcagtt acgccaccat gtgttgccca
    17881 tctcgtatgc ccaataccca cgcttccaga caatgtgtct agttttactg tgttgtttac
    17941 ttccgaaact ttgccaactc cttttcttac agagatttga ttctccgaaa atgtcgcaac
    18001 tcctacacta tcatatcctc tgtattccat acgttttaga cccttaacta aaactggtgc
    18061 cgctaatcct tctcctagat aacctatgat tgaacacatg tgtggtatta cctgttatcc
    18121 aaataataat aattgcgtat aagtaatctt attcttgtgg ttgttctgct actgtggctt
    18181 caaccatttg ttgttttggt tgactcacat ttacgcctgc cgcaagatgt atctgactct
    18241 cttcctctgt ttttactgtg tatgatggca cggtaatgac tctgtctcca atcattacat
    18301 ggccatgaac cactgcctgt cttgcttgat atgggctttt tatcaaacct ttcttctgaa
    18361 caattgtttg taatctacga gaaagtaaat ctgttacttt taaattcaaa acatcatcaa
    18421 gtgtagaatt ttcatttact aaacctattc tgatcaacga cttcatcaaa acttgctctt
    18481 ttttttctct tacttcttga gttaaagcta agagtgatct tgcttggttt ctaactctgg
    18541 aaagttctgt atgtgctttc cataactcgc gtttattttt taatccaaat gttccaagaa
    18601 cataaagttc ttcatttaac aaatcataat ttagtggacg cttaggtttt ctccagatct
    18661 tacgagaatt tttaggatct cccatctcat acacctactt tgttggtttc ttctccgtcg
    18721 gttttgctgg cgctggcttg gcatcttttg tagcagctgc tggagctgca gtctttgcac
    18781 ctgctgctgg agctgtagtg gctgctggag ctgctgcacc ttctgctgga gctgctacac
    18841 cttctgctgg aactgctcct ggaggaagaa ctttgcctcc cttgcgaact cccacggcac
    18901 caccttttct tccagtagtg cgagttctct gtcctctaac ttttaatcca taagtatggc
    18961 gaaatccgcg ccagctgttt gtattttttt ctcgttcgat atcatttcga acattaaatt
    19021 ctatatctga agtaataaga tgcaagtcat ttccagaatc aatatctttt cgtctattta
    19081 gaaaccaaga cggtatgtta agcgaagatg gattcttcat ggccttttct attgcttcta
    19141 cctgcgattc gctaagaaat ccaacattac tattctgatc tatcttgagc acatccaaaa
    19201 tggctttggc aaaattatag ccaattcctc ttacttggct taccccgatt atcattttct
    19261 ttgctcctgg aacatctttt ccagcaatcc taacgatgtg tctatattct tgtgaagcca
    19321 agtgaaaaaa tttcctctac ccccgataaa aaccatgcta aaacgaataa tactttaaat
    19381 cgcattctga cataatttac taaagtggga caagaaatat ctgaacttgt aaaacgaagt
    19441 tattcttctg taccaaaata caccgaaatt atggcaggat ttcctgaaga tcatgaacaa
    19501 ataaagacac ttggtgatct tttagaaatc aattacaaaa ttgttggggc aaaagaacaa
    19561 ctaaggcaaa acttgatttc aaaaattcaa accaactctc caaaatatcc gggaattata
    19621 ggctttgatg atgatgtaat cccagcactt gatagggcaa ttttatcttg tcatgatgta
    19681 atgttagtag gacagatagg gcaagcaaaa acaaaaattg ctgagaccat agctgaaaat
    19741 cttctatctc caatacccgt aattaaagga agcataacaa atgattcccc agtagatcta
    19801 cctgcagatg aactaatttc tctattagaa gatcaagatt atgtcatggc tactccaaaa
    19861 ttttacattg attcaactag tatagaaaaa attcgtaaca ataaacttga tactcaaata
    19921 gaatgggttg aaggaaaatg cagattcaag tatgtacttg caactcccga tatctctgtc
    19981 aaggatctgg tcggacaaat agacgctata aaaattacaa aaaaaggaat cgagatgtat
    20041 caaatcgaat catattcacc tggtcaatta atgcaagcaa agcatggaat cttctgtata
    20101 gatgaattgc ctgttcttga tacaagaaaa caagtcgcat tactttctgt actccaagaa
    20161 ggaagattca ctacaggtgc ataccctgta atttttgaac caaaaactgc tttttttgca
    20221 accgcaaatc caattgatta cactcattct ggtaaaataa tagagccatt atatgataga
    20281 ttgagaagtc atattcacac tcattatcca aaatcaatag aagatgaaat gataattata
    20341 atacaagaag caaaaatttc aaaatctttt gtgcctattt ttatgttaaa aattctagca
    20401 agaataattc aaaatgcacg cacaagtcaa gaaattaatc aagataaagg agtcagtgta
    20461 agaatgggag ttcattgtct tgagcttctt attggagaag cggaaagaac cagatctata
    20521 ctgcataaca taatggcaat tccaagacca tgtgatattt tctgcatctc tcaagctgca
    20581 aagtttgaat tgacagaaat ggatgatacg gttgccaata gagaaaaagt actccgagat
    20641 ctcattatga gctcccttaa agaatattca cttgaataca taaaagacat tgatcctgta
    20701 ctgttagaga aaataaaaca agaatttgta ggtaagactt ttcaggtttc acaaaaactt
    20761 gttggaatga atagcataga agcatcatac gaaaaccaat tacgtggctt tgagactatg
    20821 aaaaatctaa ttcataaaat atttgaacgt gtacgtgaag aacacagaga atttgaggaa
    20881 aagacacaca agtatgggat caattctgaa ttgctattac tatccgatca aactagcaat
    20941 gaactccgag ctgcaatcac cgaaattgta ttagaaggat tatgctggat tgaaccaaaa
    21001 atactagaca aaaaagaaga tgggtatgtc gcaacctgat gctttcagtc ttctctattt
    21061 tctaaatcaa gaaaaaaaag acaaaaaatc gaatgatgaa atatccgatg atacactcca
    21121 aaaaattctt caaactctag ccaaacaagc actaaaagaa aaaactatga acatgcagga
    21181 tcttgaacgc attctacaag aaagcatcag tcaaccaaat gaacagcaac ctcaagaaaa
    21241 aaactatgaa aacacagaga cagaaatcca tggaagcgga gaacctatta caaaatactt
    21301 gttcaaaaaa ggctatctaa aagacgaaaa aaaatggtta acgaataaag gtttctttga
    21361 aattggtgga aaaatcctta ctgacgtaat gaagacaatt aatgaaggag gatcaggatt
    21421 acatgagacg aaaaagattg gctctggcaa tgttattcaa gatacatcaa aaaaatttga
    21481 acttggcgat gatctaaaat ttcttaatgt tccacaatct gtgttaaatt cggtacaaag
    21541 aatagcaaaa aagaattcag aaattaaatt cccgattgag atgaaactcg aggattttga
    21601 agaatttgaa acaactgaag aagtaaaggt tgcagtagta tattgcattg atctaagttc
    21661 caccatgaaa tattctattg gtgcaggtga aggaagtaga attgaagctg ctaaaaaggc
    21721 attatggagc ttgtatgtgc taaacaaaaa attctttcca aatgattcga tctatgtggt
    21781 tggatttggt tcatttgcat ctgaagtaaa ccctcatgat attccatact tgaaaacata
    21841 tgatgcaaat gataatttcc tacattatac aaattatcaa gcagctttaa gactggcact
    21901 taagattcta aaacaagatg gggctaaaaa taaacgaata gtggtgataa cagatggtca
    21961 accaagcgcc tgtttcattg ataacgaaaa tcaaaaaaat gatattcttg cagaaaaacc
    22021 atattctcac ttttacaaac ctgatgacgc cacactttta aaaatacaaa atgaacgaga
    22081 actgaaacta gatacaggta atggtcaact ggtataccta tgttataggt ataggcaggt
    22141 cgatccattc atagactcta aaactctctc tgaagcaaaa aaatgtaaac gcgaggggat
    22201 agaaattgat accataatgg taagtgaaga agatgaactg ttaacttatg tagaaggact
    22261 tgaaatgcaa ttgaagggaa gagcctatta tatcaatccg ggaaacattg atcatgttct
    22321 aataaacgat ttcatctcaa ataaaaagaa ggttttaaac tcgaagcaaa catggtaaca
    22381 tgaatgtcag aaaattatga taagcaaaag atgcttgaga cattgactga tcctgaagtg
    22441 tctgcaatac tgttagagct tgaagacaaa agtaaagaat tatcatttct gacagacaaa
    22501 ttacaaatga cagaaaatga aatatatgaa aaattatcct atgtgataca atatgggttt
    22561 gtccatatac aaaaaaacga aaacagcgtg atttttgtgg tagatcaaga aaaacttaac
    22621 aagatcatgg aaagtgatga aaacttttct ggcgtcgtcg atggtcttac caaattagat
    22681 tcatttctta attgacatct gttttcccgc gtgacttgat aaaataaatt attccaacca
    22741 caagaccaac taatcctatt attattacga tgaatccaaa aatgcttatt gttaaagatt
    22801 catcttgtgg tagatttcct atgacagctg taacatcgat ttctcgctca ctcaaattct
    22861 caactaaaag tttgaaagtg ccactggtta aaatctcaaa gttatcttga agaggactgt
    22921 gttctatggt ttttgaagta agaatttgtc cagatggatc aaaaacagtg gcatctacac
    22981 gatcttcatt cttaaaatct gtaatttgta ttacataaac gccattttca ttagtggaca
    23041 aatcaagatc ttttgttatt tccatagaca tgcctattcc cagacgctgg tcttgtgttg
    23101 cgagattttc aattattagc tgagacccat atactgaaag tccaatccca aaaactacca
    23161 aaaatcctcc tattacaaga agtaataatg gttttttcat agcgaaagtg cgacaaaacc
    23221 cttagttaaa acttgagtta tgataataat ttagcctaag gtaacaaatt tagcctaggc
    23281 taaattataa ataatctata cacattatca aactcaaatg cacctagtga aaacaatgac
    23341 tcaacggatg tttttagctt tgataatcgc atgtgttata tctgtcatct attttgtttt
    23401 gccagttttt gatattgcgg cagaaaatga acccaagact tttctaacac attctggagc
    23461 tttgataaaa actaccggtg atgtacttga tccaataggt tattctggta tgccattaga
    23521 attcaatccc gacaaatact tgcgggaatt tagttatggg cgaacatctt cactttcaga
    23581 tggtactgtt gttcgcgaat ttacaataat tgcagaagat gacaaaataa tggaaatttc
    23641 tcccggagtg ttttacaatg tatggacttt taatggaact gtcccagggc caactatacg
    23701 agctacggaa ggagatctgg tcagaataca tttcataaac aatggaaaaa aatcacacac
    23761 aatgcatttt catggaatac atgaagcaga aatggacgga gttttggaac cagtaggaca
    23821 aaatggaggg cgatttacat acgaatttgt tgcaggacct gtagggattc atccttatca
    23881 ttgtcatgtg atgccacttg aggaacatat ttcacacgga ttgtatggtg catacatcgt
    23941 agatccaaaa gagggaagat ctactgctga tgagatggta atggttctaa atggttttga
    24001 tacggacttt gacactgaaa acaattttta tgcagcaaat acaataccat tttactatca
    24061 acatcaccca attcaaatca acacaaatga attgattcga atctatgtag tcaacatttt
    24121 agaatttgac cagataaaca atttgcatct tcatggaaac ttgtttgatt actatcctac
    24181 aggcacgtat agaataccat ctgcatacac agatgtaatt acattctctc agggagaaag
    24241 aggaatcatg gaattcagct atacttatcc tggaaaatac ttgtttcatg cacacaagac
    24301 agaatttgct gaaaagggct ggactggtct ctttttagta aaaaactcta cagaaaataa
    24361 aaatttgtat ggtgatcagt aatggaagaa gccaaaaaaa catcaaaagc taaggttgtt
    24421 gtaactgcta taattccaat tgttatttta gctggcatga tcgcatatct ttttggtcct
    24481 ggatctaatc ttcttgattt tggtgtttca cttccagaga ttacgataga gaaagtttct
    24541 tttcatgatt caaaaattgt gattactatc agaaatactg gacctataga agtagatatt
    24601 gtacaggcag atgtaaatga caggatacag ccagccgcaa ttgaacctga taaacatctt
    24661 ggtagatttg aaactgctca agtgatcatc ccatttgaat ggaatgaagc tgaaccatat
    24721 gaaataggga ttacagtaaa tgatggaaca agatttgcaa aatctgtaac ggcagctgca
    24781 ttatcaccag agcccaactt tgagcttgca tcctactttg cagtaatagg tatctatgtt
    24841 ggaataattc cagtgttgat aggactgctc tggtttccat ttatagcaaa aatgagcaaa
    24901 agcaagtaca aattcttcct tgctttgact gcaggattgc tactgttttt gggtattgat
    24961 gcaattgtag agggtctaaa gatttctaca gaaaatcttg ctgattcctt caacggtcca
    25021 ttacttgtag caacagttgt tgtgatttca tttctgggat tgtattattc atctgaaaaa
    25081 ttgattggta gagtaacttc ttctgcgaac aaacctgttg taattgcttt gatgatagcc
    25141 ataggtattg gtttgcataa tttgggagag gggctagcta taggagctgc gattagtctt
    25201 ggcgaaattg cactaagcac ctttctaatt gtaggattta ctatacataa tacaactgaa
    25261 ggtcttgcaa ttgcggcacc aatggcacgt ggaaaaccaa tgattggaaa gcttgctgca
    25321 atgggaatga ttgcaggaac accagcaatt tttggtgcat ggatgggagg atttatttat
    25381 tctccatttg ctgctgtggt atttctgtca ataggtgcag gtgcaatatt tcaagtcgtt
    25441 atctcccttg taaattggat gaggcaagat ggcgaaaaaa tattttcaac cgcatctgta
    25501 atgtctggat ttgcaatagg aatgcttgtg atgtatctga caagcatttt tgtttagatt
    25561 gaaatcttgg acctaaatac atgaacatgt acaaatcatg cgacaacaat atttgacagt
    25621 tccccgttct ttagctataa aattgcgtaa atatgttatc atgttgttga ttctattcat
    25681 aattgcctca ttttcaatta tctccgtaga ggcacagtct agtcatccta atctttttgt
    25741 atctgcagaa aacaatactt ttgaaaacca ctttttcgga tcgatggtaa ttgaagtgat
    25801 aatacgtgat aacaacatat cagatactga cagtggtatt ggtgaacccg atgtcacatt
    25861 gaatgggaat aagctccgca tggtacaggg ttctgatgga aactggtatg cgtattttgc
    25921 caatctgcaa aaagccaaag cggctgatca gatcgttcct cttgctgttg caagcaagag
    25981 tctggatttt ggtgtttttt gtgctgaatc tactgatgca tcagtacttg gtgcgtcttt
    26041 ctcgggcact gagggtgtag caattcctga tgacaacggc attaccggtg caactcaagg
    26101 tacttcttca tttactgcat gtactggtgt cttgggcaca ccttcaaatc aaaacaatgt
    26161 gattcgaagt tctagaagta tcaacttgaa tccatctgtg tctccgggtc agattggtat
    26221 aaatccaaac gcttggccaa ttgttcaact tttttctttc aatgacaacg tcgtaataat
    26281 ttacaataaa ggcggtggtg cacaaaaagt agaattggtt tattctgaca ttccaaacat
    26341 ctcctttaat cttgacagaa attcatatcc taaaggatct gaagtctttg ccaccataaa
    26401 tgacatgcaa ttaaatcaag atcctacttc tgtagactct tggactttca acgtaaattc
    26461 tacgcgtgca actttctatc aggctttcac tgaatcaggt tcaaactctg gaaatggggg
    26521 attaggacta gtaaatctaa atccatatct tgccagtcta gattttgaaa aaaatggaaa
    26581 tgtggggctg aacctaggtt ctgtagtaca acttaggaca aatcagtttc aacccacgtc
    26641 ttctgtgact acgggaacaa cggtatatac aaaaatagta acatttgtag aaacagaacc
    26701 aaattctgga atctttgaaa gttttgattt taacgatgaa tctacaattg gaatttcaaa
    26761 taatgccccg cgtggacaat cagattctat agaatataac tctaaatcta aatcaataat
    26821 atctggttct tttactgcat cactttcatt tgtaacacaa agtggaaaat ttaatcctgg
    26881 acaaaaagca actatcacat taacggataa tgatcaaaat attaatccag gaacaagaga
    26941 acatctcgat gtattcagaa gctctgcaat tattccaaca ttgcaaatag gaaatccagt
    27001 aacactagaa aaaacatcaa gtgtaatatt ttatccacaa tcaagctctt ttgtaggtgg
    27061 cacatcattt tcagcaacgg ttcctgataa aaactctgat aggttagtaa tccctgctac
    27121 cgcatctcca gacctatcat ttgaaaaaat cacactagat cttggaatga cgacaaacac
    27181 actacacaat ttatttatag acgtaccaac ccagagcggt actaactgga taaattatga
    27241 tcttcgctca tttgagcagc aattaggaat aacctcattt tctgatacaa aaatggtatt
    27301 atattttggc agcttgggtt caagcccagt tcaaattctt ggagcggggc aaatttcgtc
    27361 tgggaaagga tttgttcaga ttagtgacag tgttattgat tctatcaatg tattgccatc
    27421 ttcttcatca gtatttctag agatggactt tgacgcgtca aataataaca taggggtagg
    27481 taccatatct gctgaaacag atactcaacc aatagttttt gatctgttct ctttcggaga
    27541 aagaaatgat aatcaagtaa acaacgcaat atacagagca gagcttgaag aaacttcagc
    27601 taattcgggt acttttactg gaacaatcga atatgcagta acaaatcaac tcaatcaatt
    27661 tgatcccgcc ttcataacca gtcttagaac tatcgatgac gaaataaaat tcctgataaa
    27721 tgatgaatta gatgatgcgc aaggaacaaa cttggcatat tctgatattg caaatgttgg
    27781 cgtaaccatc ggcatctcct caaaaacaga tatcaaaaca cacgccggta cagttactct
    27841 tgaatcccag acctatagat ttggtcaacc agttgttgta gttttgaccg atccagatct
    27901 aaatctaaaa cacgatgtgg ttgacgttta tagtgtagtc aatgatcctt catcagatgc
    27961 agatgacact gtaggggata ctagtggaaa tactctcctt gaaatagaaa tcaagggatt
    28021 cagatatcat cgatgtacaa taaatgggat tgaaactggt gggcttggtt ctacaggatt
    28081 tacgcttgtt gagactggtc cagctactgg aatattcaaa ggtgcattca aaatgccttc
    28141 tcaaatatgt aacgaaagta gaacaaaatt aatctcccct gcagggggaa gcatagaagt
    28201 aaattatttt gattttaggg atgattctgg acaaccgcga gaaataggac ttacatttct
    28261 aaaaccatca tctaaaccac aaaaccaatt tgtagtagaa ggtgagcctg cgccaaatat
    28321 tgacataaag cctgtacaga tgaaagatag ttttgatagg ccaatattga agccagtgat
    28381 aggtcaaaaa attaattttg taaccgagat attaaacaat gatgaaaaat cacagagata
    28441 tacatacatc atacaagtaa aagatgaaaa taacaaagtt gttgatttac gctgggttga
    28501 gggatatgca gatccagcaa agaaaaaaac tgcgggaatt ttgtgggaag taacttcttc
    28561 aggtaactac actgtagaaa ttttcctctg ggatggaatt gattcagctt tgccacttga
    28621 caaaaaaaca gaatatcaac ttaatgtagg ttcttaattt aactatccat gtttttaata
    28681 gtgcgttgta ttgctagcta aaaattatat ttttctttat atggtgatac tgtacgtaac
    28741 tctttaacca ctttttttaa aatccgaacc gtttcatcaa tctcttcttt tgtatttgat
    28801 attccaatag tgagtcgtaa cgaacccatt atttgttcat gagaaaaacc catcgctttt
    28861 agaacatgtg atgctttttg tactctgaca gaacatgcag atcctgtgga tgcagcaatg
    28921 ccattttcat caagcttaat tatcaaatcc tcaccgttta caccaagaaa tgtaaaatgt
    28981 gtattgtttg gtattctttt ttcaagatga ccgttaactg tgacatgagg aatttccttt
    29041 attatttgtg aacaaagcat atttccaagt tttttaaaat gttccagatt ttgttccatg
    29101 ttgtcctttg caagttgaca tgcctttcca aagcccacaa ctcctgctac attctctgtg
    29161 ccagatcgaa atccattctc ttgtccgcca ccaagtatga tgggatctat ctttatgcca
    29221 tttttgacat ataatgcgcc aactcctttt ggaccgttta tcttatgaga tgaaattgac
    29281 atcaaatcta tgcctagttc cttcacatct acaggtgtct ttcctactgc ctgaacagca
    29341 tctgtgtgaa aaggtatgtt tttttcatga acaatttttg ctatctcctt gatcggttga
    29401 actgttccaa cttcattatt tgcaaacatg atactgacta aacatgtatc tgatgaaatt
    29461 tcactttcca gaacttctag attgacaaat ccatatctat ctacgggaag atatgtcaca
    29521 tgaaatcctt cgctttcaag ttttttgcat ggctccaata tggcatcatg ttcaatcgaa
    29581 gatgtgatga tgtgtttgcc tttatttttt cgtgccattc cataaagtac cgtattattt
    29641 gattccgtac ctcctgatgt tatcaaaact tcttttgggt cagcatttat cagagaagca
    29701 attctttttc gcgcattttg aattgctaca cttgccagac gtccatattt gtgaattgat
    29761 gaaggatttc catactgcgc tctaaaatat ggcatcatct cctcaacagc tctttcatga
    29821 accggagtag atgcagcatt gtctagatat atcaatctgc aatcacgttg agcttgcctg
    29881 gtctatatcc atcaggatct attgtgatag ttctgaatcc aatttgtttt aacctagaat
    29941 ccaattctat taatttgatc ttatctgaaa gtaatttgat ctcctctggc ataacttcaa
    30001 ttcttgcatt gccatcaaga tcccgtatac gaacttgtct tatgccaaac aactgtttta
    30061 cagttgtttc tccaatctca attcttgcaa gcttctctac agtcacttgc tgtccccacg
    30121 gaatgcgtga agctaggcac gaatttgaag gtctatcgta aactgaaagc cctattcttc
    30181 ttgcctcttt tcttatctca tcttttgtaa atcctgtttc taccaatgga ctctttatcc
    30241 cattttcttt tagtgctttt attcctggtc tatagtcacc aagatcatca aggttagtac
    30301 catcaactat cagatctatc ttctctttat ttgcaatttg ttttagatgt tctgacaact
    30361 ccattctgca atgaaaacac ctatttttgt cattttttac aaaatctgga tttttcagct
    30421 cattgtattc tattatgata tgctttattc caatttcaac acttattttt tttgcagatt
    30481 ctaattcgtc ttgtgctagt gttttataat cggcagtaac tgctactgaa gatgtgccaa
    30541 gagatcgata cgcagcatat gcaacaagtg cactgtctac cccacctgaa agtgcaacaa
    30601 gtgcattttt tttatcatca aaccaatgtg ttaattcatc aatttttttc attttgtatc
    30661 gattacgtct ttaatctgag atctgattat atcatatgca tctttaaatg ataatgacat
    30721 tgactctgcg atggttttga tatctgcaga ttctactttg aagtgctttg ctgttccctc
    30781 tctgttaact attttgcatt ttaccgaaaa tctttttcca tgaatagtta ctggtactgt
    30841 gactattgtt cttggtacga tgtatcttgc agagtttctt actctaacgc ctaacgtgcc
    30901 tgtttctgat accaatgtat ccagtatgtt gtttactgta ttagaatcac atattatgga
    30961 tacaagatga gtcggtcttc ctttttttgt tatggctgga gctaccgtaa cgtctttggc
    31021 tccatttgat atgactttat caatcatatg acctaacacc tccccagaca catcatccag
    31081 atttgtctca agtatttgca ctgtatcttg ttgaaactga ttggtatttt caccacgtac
    31141 aattttaagt acatttgcaa acccatcaaa attctttttt ccagcaccat atccaataga
    31201 tctcactttc attgagggat aaaattctga acacctgtct gccaaattga caagaagact
    31261 tgctccagta ggagtagtta tttcttcttt cacttgtccc cctgatatga tgatgtctgt
    31321 acccataaaa atctctaaaa ttgcacttgc tgggtttgat acggttccat gtgaaaaatt
    31381 aagcattcca ccacctatag ctacaggtgt agaaactatc tcgtttgaaa aaaaatccag
    31441 atcatctaat gcaatggcag aaccaattat atccacaaca gtatctatgc ttgacgcttc
    31501 atgaaaatga accgactcta caggctctcc atgaattttt gattctgcat ttatcagtga
    31561 ctttatactc tcttttgcaa agactttgga tttttcagat aacccaatct tgtctgaagt
    31621 aagcaggata cattcttgaa tttcaattcc ttttcgctca tacgtatctt ctcttatatc
    31681 taacaaaagt tctgtagctt ttgttccatg ttttgttacg ctgccaaaat ctattttctg
    31741 gattctagat cctgtcagat aattttctgc aacatgtatt ccatcaataa cttttgattt
    31801 ttttgctcca atatctatca gagcagaaag taacatgtct cctgaaatgc cagctacttg
    31861 tgaatctatc accactacca tgaaagtgca aacctcttag aatgcatata aattgtaatt
    31921 ttaattcata atgagattaa aaattgaact cattttgatt tcaaaccaat tatttactat
    31981 gaaaattgta gcaaatataa ttaaagaaat tcataattta caaataaaac atgctatttt
    32041 tttataattg gcaatttata atatgggaat cttaaaaaaa catacttaaa taattgcgta
    32101 tgagtaaatc tcacatgtgt at
//
LOCUS       DATIDL010000034         3748 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrosopumilaceae archaeon isolate SMAG_U16342
            k141_1862806, whole genome shotgun sequence.
ACCESSION   DATIDL010000034 DATIDL010000000
VERSION     DATIDL010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935836
            Sequence Read Archive: SRR25158326
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosopumilaceae archaeon (soil metagenome)
  ORGANISM  Nitrosopumilaceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrosopumilales;
            Nitrosopumilaceae.
REFERENCE   1  (bases 1 to 3748)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3748)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/04/2024 05:09:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,520
            CDSs (total)                 :: 1,474
            Genes (coding)               :: 1,474
            CDSs (with protein)          :: 1,474
            Genes (RNA)                  :: 46
            rRNAs                        :: 1 (5S)
            complete rRNAs               :: 1 (5S)
            tRNAs                        :: 43
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3748
                     /organism="Nitrosopumilaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1862806"
                     /isolate="SMAG_U16342"
                     /isolation_source="soil"
                     /db_xref="taxon:2202732"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            171..293
                     /locus_tag="VLB45_01580"
     CDS             171..293
                     /locus_tag="VLB45_01580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04428.1"
                     /translation="MASPDVAAFASWSVITAVFIVIVSLVYKKFRRQKDEPPSS"
     gene            274..837
                     /locus_tag="VLB45_01585"
     CDS             274..837
                     /locus_tag="VLB45_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF99 family protein"
                     /protein_id="HSD04429.1"
                     /translation="MNHLHLEKKGLRGLAVAESFRLGDKLSYLAGVVMRRDFIIDGFI
                     FGTSTIEGDDATDSILLMYQNLNRDDISFVLISGLIISMYNIVDIKKIQNKLKIPVIG
                     VTYEDSKGIEAAIKHHFSDSYQSKIDKYKNLGERTKISLHTGHDLYVRTEGCTIQEAK
                     TLLDAFTLQGSIPEPLRVAQMLAKTRL"
     gene            complement(838..1224)
                     /locus_tag="VLB45_01590"
     CDS             complement(838..1224)
                     /locus_tag="VLB45_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007401350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04430.1"
                     /translation="MGDSETFAVEKGHGEKAVQWMNEEAKKQGWKFEARLYGYEIETK
                     NFGSFEMFSWIGDVKAARDLVIKASKRFKIRVIEGGYKAREIMLKLERSDYAMVRKGE
                     RVIGHIEFSSPRIKGSKWIVKLEERR"
     gene            1319..2479
                     /locus_tag="VLB45_01595"
     CDS             1319..2479
                     /locus_tag="VLB45_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04431.1"
                     /translation="MDRTAADIRKAIESNWKEYLSRYGNLFSSNFISGSSPPSVFVGS
                     YGYPKVSVGPMVPPVHGDTTMLDSPENWLGKSLEAIVNYRLSLVRGIQNVSVLEPQGK
                     YIESLQEVAMASRPIDSDVEFLKNTIPITSIDGENAPFGPIGEIKTAKFSNSSSDKNI
                     QKLYYARDLLAQDSVLDLYNRGIEITRIQKCFSIGMFGKNRKLVPTRWSITATDDIIS
                     KSLVSEIMECDIIDSHRVFSFNHLGNFFAVIVFPSRWMFEMQEAWYDQQGNIGFGSDF
                     EDARGLDHYPVTAGAHFASRLGVAEYLYKNKVQAAVMVLREIRPEYAIPLGVWQVREG
                     VRMAMKQKPDIVSSFEESLDLACKTMSIGEREWLAKSKLCQARRQKSIADFF"
     gene            2519..3448
                     /gene="artG"
                     /locus_tag="VLB45_01600"
     CDS             2519..3448
                     /gene="artG"
                     /locus_tag="VLB45_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thaumarchaeosortase"
                     /protein_id="HSD04432.1"
                     /translation="MQDKSILYAILLIASPIIFALVVFPDSFSLSWNQGRGGFLFALA
                     FIVAELVGLKLGIAKKRLLAVVPLAVLTMVYLVSLEFGLRDYIISVAPNYNVQLIYSW
                     TWLWDFVVLSIFLVSSMSILFGKKWIRISPAGPIFIAGSAVILSLDAFFPYDTLGPLQ
                     YVVPYLVQTNAWIINALDLGTASARDNLMFLRGDHGPFALQVFWPSAGVHSVIIYSLV
                     MMAFLFKMNIPPKRKAVYFVLGIIGTISVNMIRIFSLSIFALKVSTNVSEFEEFHSVA
                     GEIMFLPWLFIFLLVVTYVETRRIKKLEHDQNK"
     gene            3432..>3748
                     /locus_tag="VLB45_01605"
     CDS             3432..>3748
                     /locus_tag="VLB45_01605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04433.1"
                     /translation="MIKINRNIKTGTYRLDDIFHDLKNAAILMDVFKTLDELDDVFSK
                     TNVIVEEKDHYMFVKNEDATIVIGYDHLKKSDSKILYLDIVHELVHVKQQRSGLDLYD
                     KSYS"
BASE COUNT         1161 a          667 c          712 g         1208 t
ORIGIN      
        1 gattcttcca cccatacctg gattctataa taaaccaaaa acaatagatg atcttgttaa
       61 tcacaccgtt ggaaaatgtc ttgatcaatt taacattgaa cacaaattgt ttaaaagatg
      121 gaagacaacc taaatgaagc tgttaaaaac agctttgtat ttcctacttt atggctagtc
      181 cagatgttgc tgcatttgct tcatggtccg tgattacagc tgtttttatt gtgatagtaa
      241 gcttggttta taaaaaattc aggcgacaaa aagatgaacc accttcatct tgaaaaaaaa
      301 ggtctaagag gattagccgt agcagaaagc ttcaggctag gtgacaagtt atcgtatttg
      361 gcaggagttg tcatgagacg tgatttcatt attgacggct ttatctttgg tacaagtaca
      421 atcgaaggtg atgatgcaac tgattcgata ttgttgatgt atcaaaacct aaatcgtgat
      481 gacattagct ttgttctgat ttcaggattg attatatcga tgtataatat agtagacata
      541 aaaaaaattc aaaacaagct gaaaatccca gttatcggtg tcacatatga agactcgaaa
      601 ggaattgaag ctgcaataaa acaccatttt tcagattcat atcaatcaaa aatcgacaaa
      661 tacaaaaatc taggtgaaag aacaaaaatt tctcttcata cgggacatga tctttatgtg
      721 cgaactgaag gttgcacaat acaagaagca aaaacactgc ttgatgcatt cacactacaa
      781 ggttcaatac ctgaaccact aagagtggca caaatgcttg ctaaaacaag actatagtta
      841 tcttctttct tctaatttta ctatccactt gctacctttg attcttggtg aagaaaactc
      901 gatatgtcca attactctct cgccttttct aaccatggcg taatctgatc tctcaagctt
      961 taacattatc tcccttgcct tgtagcctcc ctcgattact ctgatcttaa atcgtttgct
     1021 tgcctttatg acaagatctc ttgcagcttt aacgtctcca atccatgaaa acatttcaaa
     1081 agagccaaaa ttttttgttt ctatctcata tccgtaaagt cttgcttcaa acttccaccc
     1141 ttgctttttt gcttcctcgt tcatccattg aactgctttt tcaccatgtc ctttttccac
     1201 agcaaaagtt tcggaatcgc ccattcttat cagatttcaa atatgtattt gaatataatt
     1261 tcaagctaat gctagttaat tgtacaatat tattagctag gatatccttt acattgtgtt
     1321 ggataggaca gctgcggata ttaggaaggc aatcgagtca aactggaaag aatacctgtc
     1381 ccgatatgga aacctgtttt catcaaattt tatcagtggt tctagtccgc catccgtctt
     1441 tgtaggttcg tacggttatc caaaagtctc tgtaggtcct atggtaccac cagttcatgg
     1501 tgatactaca atgcttgact cgccggaaaa ctggctgggt aagagccttg aagcgattgt
     1561 aaactataga ctgagccttg taaggggaat acagaatgtt tctgttttag agcctcaagg
     1621 aaaatacatt gaaagcttac aagaagtagc tatggcgtca agaccaattg attctgacgt
     1681 ggagtttcta aaaaatacga ttcctattac atccattgat ggcgaaaatg caccatttgg
     1741 gccaattggt gaaataaaga ctgccaagtt ttcaaactct tcatctgaca aaaatattca
     1801 gaaactctac tatgccagag atttgttagc tcaagattct gtattggatc tgtataatcg
     1861 tggtatcgaa attacaagaa ttcaaaaatg ctttagcatt ggaatgtttg gcaaaaacag
     1921 aaagcttgtt ccaacccgct ggagcataac tgcaactgat gacatcatat caaaatcatt
     1981 ggtgtctgaa ataatggaat gtgatataat tgacagccac cgtgttttct catttaatca
     2041 tcttggaaac ttttttgcag tgattgtttt tcccagcaga tggatgtttg agatgcaaga
     2101 ggcttggtat gatcagcaag gaaacatagg atttggttca gattttgaag atgctcgagg
     2161 tcttgatcat tacccagtta ctgcaggagc acactttgca tcacgactgg gtgttgcaga
     2221 atatctttat aaaaataaag tacaggctgc cgtcatggtg ttgcgtgaga taagaccaga
     2281 atatgcaatt ccacttggtg tgtggcaagt tcgtgaaggt gtgcgtatgg caatgaaaca
     2341 aaaacctgac attgtatcaa gttttgaaga gtctcttgat cttgcatgca agactatgag
     2401 cataggcgaa agagaatggc ttgcaaaaag taaactgtgc caagctagac gacaaaaatc
     2461 aattgcggat tttttctaac tctagagttt ttatttaccc ctaattgctg ggtaaataat
     2521 gcaggacaag agcatacttt atgcaatttt acttattgct agtccaatca tatttgctct
     2581 tgtagtgttt cctgattctt tcagtcttag ttggaatcaa ggacggggtg gttttctttt
     2641 tgcacttgca tttattgtag cagaactggt aggactaaaa ttaggaatag caaaaaagag
     2701 attgcttgca gtagtgccac ttgctgttct tacgatggtt tatcttgtta gcttagagtt
     2761 tggtttacgc gattatatca tatctgttgc tccaaattac aatgttcagt taatctactc
     2821 atggacttgg ttgtgggact ttgtggtgct gtccatattt cttgtctctt ccatgtcaat
     2881 tttgtttgga aaaaaatgga ttcgcatatc tccagcaggg ccaatattca tagctggcag
     2941 cgcagtaatt ctatcccttg acgcattctt tccatatgac actctgggac cattacagta
     3001 tgtagtacca tatcttgtac aaacaaatgc gtggatcata aatgcattag atttgggaac
     3061 tgcctctgcg cgagacaacc ttatgtttct aagaggcgat catggtccat ttgcgcttca
     3121 agttttttgg ccatctgcag gtgttcacag cgtaataata tactcgcttg tgatgatggc
     3181 atttttattc aaaatgaaca ttcctccaaa aaggaaggct gtctattttg tattaggaat
     3241 aattggtaca ataagtgtca atatgatcag aatattttca ttatctattt ttgcattaaa
     3301 ggtatcaact aatgtgtcag agtttgaaga atttcattca gttgcaggag aaataatgtt
     3361 cttgccatgg ctttttattt tcctacttgt tgttacatat gtagaaacac ggcgcataaa
     3421 gaaattagaa catgatcaaa ataaatagaa atataaaaac tggaacgtat cgtcttgacg
     3481 atatttttca tgatttgaag aacgcagcaa ttcttatgga cgtttttaaa acgctagatg
     3541 aacttgacga tgtgttctca aaaacaaacg tcattgtaga agaaaaagac cattacatgt
     3601 ttgtaaaaaa tgaagatgca acaattgtaa taggatatga tcatctgaaa aaatcagatt
     3661 cgaagatttt gtatcttgat atagttcatg agctagtgca tgtaaaacaa caaagatctg
     3721 ggcttgatct ctatgacaaa tcttattc
//
LOCUS       DATIDL010000035         3565 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrosopumilaceae archaeon isolate SMAG_U16342
            k141_1863486, whole genome shotgun sequence.
ACCESSION   DATIDL010000035 DATIDL010000000
VERSION     DATIDL010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935836
            Sequence Read Archive: SRR25158326
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosopumilaceae archaeon (soil metagenome)
  ORGANISM  Nitrosopumilaceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrosopumilales;
            Nitrosopumilaceae.
REFERENCE   1  (bases 1 to 3565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/04/2024 05:09:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,520
            CDSs (total)                 :: 1,474
            Genes (coding)               :: 1,474
            CDSs (with protein)          :: 1,474
            Genes (RNA)                  :: 46
            rRNAs                        :: 1 (5S)
            complete rRNAs               :: 1 (5S)
            tRNAs                        :: 43
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3565
                     /organism="Nitrosopumilaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1863486"
                     /isolate="SMAG_U16342"
                     /isolation_source="soil"
                     /db_xref="taxon:2202732"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..220)
                     /locus_tag="VLB45_01610"
     CDS             complement(<1..220)
                     /locus_tag="VLB45_01610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04434.1"
                     /translation="MKIGLVAACIAGAIVVILIAMTYAMQGPAIQYSIFLDPFKDPQD
                     LFTTARVTIQNNGNAPLTNVLIDYGGNFE"
     gene            complement(252..497)
                     /locus_tag="VLB45_01615"
     CDS             complement(252..497)
                     /locus_tag="VLB45_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007401523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HSD04435.1"
                     /translation="MEIAYVLVKSKMSHEMEVMNDILKINGVKEVMGTYGVYDLFVKV
                     QAQTSRDVERIITHQIRKIKNVLSTTTLSVIPEQGGK"
     gene            complement(500..1936)
                     /locus_tag="VLB45_01620"
     CDS             complement(500..1936)
                     /locus_tag="VLB45_01620"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007401522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HSD04436.1"
                     /translation="MVKENCGVVGIFSLDGANVIPMVIDSLRALQHRGQEAWGIAIPK
                     KDPYKQVGLVSSAASDFDKVTREYASPAAIGHVRYSTMGKSNLENAQPLKVKDLCIAH
                     NGTIANVEELAGMVGGCSFTPQNASDTLVAAKRLVSLMATNGNLGNALSILKNEMVGS
                     FCFTFVSDDNAVYAARDPKGFRPMVIGFRKDINTYIIASESAALSAVGAQLIRDVKPG
                     ELIKISEAGLESEMFSEEKSHSHCSFEFTYFAHPSSMMEGTNIYLARKRIGQYLARKF
                     PIHDADIVIPVPDSARPAALGYAQELGISFEEGLLKDRYSRKGPLRSFIEPHQSDRVE
                     INRWIIPITAVIDGKHVVVVDDSLVRGTSSKAIIKALRRAGARKISMVITYPPIKFPC
                     YAGIDFPSQEELVTFMDDGKEYSETALTEKVRQIIGADFLGYNDEKNLAEAVGMDSGS
                     MCFTCSTGNYAPLGIKPVFKSRAEIKGE"
     gene            complement(1929..>3565)
                     /gene="purL"
                     /locus_tag="VLB45_01625"
     CDS             complement(1929..>3565)
                     /gene="purL"
                     /locus_tag="VLB45_01625"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HSD04437.1"
                     /translation="NKLIRNRADKDDFVVLVGGSTGRDGIGGSQFASDKLESENRSAV
                     QIPDPFIEKLVIEAVLEARDKGCIKAMKDLGGGGLSCAISETADALSVGIEMDVARIH
                     KRETGMSPSEIMVSESQERMLLITDRYKLQLLKEICEKFHVPYSVIGRIKEDRIMQVK
                     VETNTIANLPSGVVANAPLLDRPSSKPKYLKKLLDVPKPNTPKDLSKTLLTMLANPNI
                     ASKIWVYGQYDHEVGIRTVVKPGQDASVLRLDNGKFLAVKIDGNSKHCYIDPRQGAIG
                     CFEEACRNVVCTGATPIGMVDHLQFGNPEDPEIFWTFKESLEGIADFARHFGIPCVGG
                     KVSFYNETDNGPIKPTPLIGVLGLIENNLFNPQKISENDLLVIIGETSDEMGGSEYYE
                     YIHHVIGGKCPTVNLKSSKDNMESILKLINSGLVNAVHDCSKGGIAITLSELCIINKI
                     GCVVSIDQIPSKKLKLDEILFSESHSRYLLAINKKNIKKTEELLKQKNIQFGIIGKFS
                     GSSIVFKEKARKVINLRIDKAQEKWLNSLGALVTNG"
BASE COUNT         1017 a          747 c          586 g         1215 t
ORIGIN      
        1 tttcgaaatt cccgccataa tctattagca catttgtaag tggagcattc ccattatttt
       61 gtattgtaac acgtgcagtg gtgaacaaat cttgtggatc tttgaatgga tctaaaaaga
      121 tagagtattg tattgccggt ccttgcattg catatgtcat tgctattagt ataacgacaa
      181 ttgcgccagc aatacaagcg gcaacaagac caatcttcaa tgatattaat tgttctttcc
      241 aattatatag gctatttgcc gccctgttct ggaattacag aaagcgtcgt tgtggaaagg
      301 acgtttttta tttttctaat ttggtgtgta attatacgtt ctacatccct gcttgtctgt
      361 gcttgaactt taacaaaaag atcatagaca ccatatgttc ccatgacttc ttttacacca
      421 ttaattttta gaatatcgtt cattacttcc atttcatgac tcattttgct tttaactaag
      481 acatatgcta tttccaaact tattcacctt tgatttctgc cctgctctta aagactggtt
      541 ttattccaag tggggcatag tttccagtag agcaagtaaa acacatagaa ccagaatcca
      601 tccctacagc ttctgctaga ttcttttcat cattgtagcc aagaaaatct gcgcctatta
      661 tttgacgaac tttttctgtc aatgcagttt cagaatattc ttttccatca tccataaagg
      721 ttacaagttc ttcttgagat ggaaaatcaa ttcctgcgta acacggaaat ttaattggcg
      781 gatatgttat caccatgctt atttttcttg cacctgcacg acgtaatgct ttgattatag
      841 cttttgagct tgttcctcta accagactgt catctactac aaccacgtgt tttccatcaa
      901 taaccgcagt gattggaatt atccatcgat taatttctac gcgatcactt tgatgaggct
      961 caataaaact acgtagtgga ccctttctgc tatatctatc ctttagaagt ccttcttcaa
     1021 aggaaattcc tagttcttgt gcataaccca acgctgctgg cctagccgag tcaggtactg
     1081 gaattacaat atccgcatca tgaattggaa attttcttgc aagatattga ccaattcttt
     1141 ttctagccaa gtaaatgtta gtaccttcca tcatgcttga aggatgtgca aaatatgtaa
     1201 actcaaatga gcaatgtgag tgactttttt cttcagaaaa catttctgat tccaagcctg
     1261 cctcactgat ctttatgagt tctcctggtt ttacatctcg tatcagctga gcgccaacag
     1321 cagaaagtgc agctgattcg gaagctataa tgtacgtatt gatgtctttc ctaaaaccaa
     1381 tcaccatagg tcgaaatcct tttgggtcac gtgcagcata aactgcatta tcatctgaaa
     1441 caaatgtaaa acaaaaagat ccaaccattt cattttttaa aatggaaagg gcatttccaa
     1501 gattaccatt agtggccata agggagacaa gtctttttgc tgcaactagt gtatcacttg
     1561 cattttgagg tgtaaaagaa cagccaccaa ccatgccagc aagttcttcg acgtttgcaa
     1621 ttgtaccatt atgtgcaata caaagatctt ttacttttag aggttgagca ttctccaaat
     1681 tactttttcc catggtagaa tatctgacat ggcctatcgc cgcaggagat gcatattctc
     1741 ttgttacttt atcaaaatct gatgcggcac ttgatacaag accaacctgt ttgtatgggt
     1801 ctttttttgg aattgcaatt ccccatgcct cctgtccacg gtgttgtaat gcacgtaatg
     1861 aatcaatcac cataggaatc acgtttgcac catcaagact aaagattcca actacaccgc
     1921 agttctcttt aaccatttgt taccaatgct cccaacgagt ttaaccattt ttcttgtgcc
     1981 ttatcaatcc ttagattgat taccttccgt gctttttctt taaacactat actagagcca
     2041 gaaaattttc cgataattcc aaattgaatg tttttttgct tcaatagttc ttctgtcttt
     2101 ttgatatttt ttttattaat agccaaaaga tatctggagt gagattcaga aaagagtatt
     2161 tcgtcaagct ttaacttttt acttggaatc tgatctatcg aaacaacaca accaatttta
     2221 ttgatgatac atagttcaga cagagtaatt gcgatacctc ctttggaaca atcatgtaca
     2281 gcgtttacca atcctgaatt tattaatttc aagattgatt ccatgttgtc ttttgaagat
     2341 tttagattaa cagttggaca tttaccgcca atcacatggt gaatatactc ataatactca
     2401 gaacccccca tctcatcact tgtttcacca attattacaa gcagatcatt ttctgatatt
     2461 ttttgcggat taaatagatt attttcaatc aagccaagaa caccaataag tggagttggt
     2521 ttgattggtc cattatcagt ttcgttgtaa aaactaactt tgcctcctac gcatggaatt
     2581 ccaaaatgtc ttgcaaagtc agcaattccc tcaagtgatt ctttgaatgt ccaaaagatc
     2641 tcaggatctt ccggatttcc aaactgcaag tggtcaacca ttccaatagg tgttgcacca
     2701 gtacatacta catttcgaca tgcttcttca aagcacccaa ttgcaccttg acgaggatct
     2761 atgtaacagt gctttgaatt gccatcgatt tttactgcta aaaatttgcc attgtctaga
     2821 cgaaggacgg aagcatcttg tcctggtttt accactgttc ttattccaac ttcatgatca
     2881 tattgaccat aaacccaaat cttgcttgcg atatttggat ttgcaagcat tgtcaaaaga
     2941 gttttagaaa gatccttagg tgtatttggt tttggtacat cgagtagttt ttttaaatat
     3001 ttaggttttg aagaaggcct atcaagtaat ggggcattag caacaacacc gcttggaaga
     3061 tttgcaatgg tatttgtttc aacttttact tgcatgattc tatcttcttt tattctgcca
     3121 attacagaat atggaacatg aaatttttca catatttctt ttagcaattg tagtttgtat
     3181 ctgtcagtta tcaaaagcat cctttcctga gattctgaaa ccatgatttc tgatggagac
     3241 attcctgttt cccgcttgtg tattcgtgcc acatccatct ctatacccac agaaagggca
     3301 tctgcagttt ctgatattgc gcatgataga cctccaccgc ccaaatcttt cattgcctta
     3361 atgcaaccct tgtctcgtgc ttcaagcaca gcttctatga ctagtttttc aatgaaagga
     3421 tccggaatct gaaccgctga acggttttct gattctaatt tatctgaggc aaattgtgaa
     3481 ccgccaatac catcacgccc agtagaacca ccaactaaaa caacaaaatc atccttgtca
     3541 gccctatttc gaattagttt gtttt
//
LOCUS       DATIDL010000036         3818 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrosopumilaceae archaeon isolate SMAG_U16342
            k141_1258366, whole genome shotgun sequence.
ACCESSION   DATIDL010000036 DATIDL010000000
VERSION     DATIDL010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935836
            Sequence Read Archive: SRR25158326
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosopumilaceae archaeon (soil metagenome)
  ORGANISM  Nitrosopumilaceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrosopumilales;
            Nitrosopumilaceae.
REFERENCE   1  (bases 1 to 3818)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3818)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/04/2024 05:09:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,520
            CDSs (total)                 :: 1,474
            Genes (coding)               :: 1,474
            CDSs (with protein)          :: 1,474
            Genes (RNA)                  :: 46
            rRNAs                        :: 1 (5S)
            complete rRNAs               :: 1 (5S)
            tRNAs                        :: 43
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3818
                     /organism="Nitrosopumilaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1258366"
                     /isolate="SMAG_U16342"
                     /isolation_source="soil"
                     /db_xref="taxon:2202732"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..324)
                     /locus_tag="VLB45_01630"
     CDS             complement(<1..324)
                     /locus_tag="VLB45_01630"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HSD04438.1"
                     /translation="MSVLFIVRTQLYLYTAPVITLTQENNIYDALLLMQKNDIKRIVI
                     VNKDVSIGIVTERDMGMFLDKDKSTRTLDQICLGEIMSKHPVTIALGQSDHLEQCAIR
                     METFQI"
     gene            complement(395..736)
                     /locus_tag="VLB45_01635"
     CDS             complement(395..736)
                     /locus_tag="VLB45_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007551648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HSD04439.1"
                     /translation="MQVQLHGKPVQEETTKDVLLGILADKYSRAILESTMDSPKSAIE
                     LSADCKIPISTAYRRVQELHDNKLLGISGSINDDGKKYFLYKSKIKSIMTCFNGSSVE
                     VEIVPNSLEHL"
     gene            complement(813..1286)
                     /locus_tag="VLB45_01640"
     CDS             complement(813..1286)
                     /locus_tag="VLB45_01640"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HSD04440.1"
                     /translation="MVNDIFKRILVPFDGSTFSKNALLLAIQISKKFDSKLFIIAAVD
                     ASDFPPGMLLGLLQKDKRLEQSVNEFVIAVKSQIRKELLAGVAVCKAKGLTEAYYDII
                     SGSPVESILKFAKGRKINLIVMGSQGLHGISKLKVLGSVSRKVSEMSSCPVLIVH"
     gene            1455..1943
                     /locus_tag="VLB45_01645"
     CDS             1455..1943
                     /locus_tag="VLB45_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008301438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HSD04441.1"
                     /translation="MDKSKGKKIDTTTNVTPFWHGWSDLDRVFDNFRRDFERAFAAFP
                     VAMPSLPSISTTSCDVIDEGNRFVVNVDLPGVKKNEIKLNVTDTAIEVSAEHKEEDEE
                     KKKNYVRKERKEVSYHRTMSLPEKVISSKATAKLNNGVLSVEIPKVTPTLKPKSTSIP
                     IQ"
     gene            complement(1960..2856)
                     /locus_tag="VLB45_01650"
     CDS             complement(1960..2856)
                     /locus_tag="VLB45_01650"
                     /inference="COORDINATES: protein motif:HMM:NF004863.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HSD04442.1"
                     /translation="MISVIGAGKVGSAIGFLIASTSLDDLVLVNHNKNRAIGEALDIT
                     NAVPKGSTISVTGTDDYELTRDSKVIVIAASRGKIAVSRTDLLADNVSLIREIVNNIK
                     KHNDDTKIIVVTNPLDVITYFTLKETQFSRKNVIGMGSSLDSSRFRYLLAKTLGVNQS
                     KIEGIVIGEHGDSMVPIFSSARYDGKPISNMLDQIKVQKITTELRNYWILLKTFKDAS
                     VFGAAKNTFDLIKAIIYDEKLQVPASILLDGEYGLSNVCLGVPVIIGKNGVDKINEIE
                     LDKSELHALVDSSRVVQNNIKI"
     gene            complement(2870..3298)
                     /locus_tag="VLB45_01655"
     CDS             complement(2870..3298)
                     /locus_tag="VLB45_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HSD04443.1"
                     /translation="MRLPKINKILVPMDGSKNSLRGLDTAIYLARQCGATLTGLYVIP
                     FYPKSLILTPILYEKELAKAGKEFLGKAKKRAAQNGIVFNEKITDGHEAEEIVGFAHD
                     KGFDLIVIGARGLGSVKEKFLGSVSNAVAHKSKMPVLIIK"
     gene            3522..>3818
                     /locus_tag="VLB45_01660"
     CDS             3522..>3818
                     /locus_tag="VLB45_01660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04444.1"
                     /translation="MNSDQSTYEYVKEVQKMPIRKALHPATIVEPSFSISKVIDMMSK
                     TDSYDVYCMNEGTVLTTNAREILTVKDAFTMKISSLLHKIRALSKDDTIEKAATA"
BASE COUNT         1247 a          698 c          605 g         1268 t
ORIGIN      
        1 aatttgaaaa gtctccattc taatcgcaca ttgttcgaga tgatcacttt ggcctaaagc
       61 tatggtaact ggatgtttac tcataatttc tcccagacat atttgatcta gagtgcgagt
      121 actcttgtcc ttgtcaagaa acatacccat atcacgctct gttacaatgc ctattgacac
      181 atctttgttt acaataacaa ttctctttat gtcatttttt tgcatcagta ataacgcatc
      241 ataaatatta ttttcctgag ttagtgttat tacgggtgca gtgtatagat acagttgggt
      301 tctcacaata aatagcacac tcatgacaga tttaaaacgc gtatttgatt ttcagtcttg
      361 gttttcatgt taataaaaag agagttttcc taacctatag gtgctctaaa gagttaggaa
      421 caatttcaac ttctactgaa cttccattaa aacaagtcat tattgatttg atcttgcttt
      481 tgtacaagaa gtatttttta ccatcatcgt taatcgaacc tgagattccc aaaagcttgt
      541 tgtcatgaag ctcttgcacc cttctgtatg cagtacttat tgggatcttg cagtcggcgc
      601 ttaattcgat tgcagatttt ggtgaatcca ttgttgattc aagtattgcc ctagaatact
      661 tgtctgcaag gattccaagt aagacatctt tggttgtttc ttcttgaaca ggttttccat
      721 gtaattgaac ttgcatagaa atgctttttt attaatcgaa tatagccagc aggaccgcat
      781 tgcaatgcag tgcaagcaaa cctcgtattg atctagtgca ctatcaaaac agggcaacta
      841 gacatttcag agacttttct acttacgctt ccaagcacct tcagcttact aatgccgtgt
      901 aacccctgac tgcccataac aataagatta atctttcgac cctttgcaaa ttttagaata
      961 gattctacag ggcttcctga tatgatgtca taatatgctt cagtaagacc tttagcctta
     1021 caaactgcca ctccagcaag tagttctttt cttatttgtg atttgacagc aattacaaat
     1081 tcatttacag attgttccaa tctcttatct ttttgtaata aaccaagtaa cattcctggt
     1141 ggaaaatctg acgcatcaac tgctgcaatt atgaatagtt ttgagtcaaa ttttttggat
     1201 atttgaatag ccaataagag tgcattttta gagaatgtag aaccatcaaa aggaacaaga
     1261 attcttttaa aaatgtcatt taccatgttt taaatcatct tagaacgtac ttaaatcaac
     1321 ctgttaattt cagaattgat aactaatttt atttaaaaaa aattcgaatg tgtatgatac
     1381 atatttttca catgtgataa tcaaaaaaga taatctgcat actcagatta atatcaagat
     1441 gtaacaagag gatcatggat aaatccaaag gaaaaaaaat agatacgaca actaacgtga
     1501 ctccattttg gcatggatgg tcagaccttg acagagtgtt tgacaacttc agaagagatt
     1561 ttgaacgagc ctttgctgct tttccggtag caatgccgtc gcttccaagt atatccacta
     1621 catcatgtga tgtgattgat gaaggaaacc gattcgttgt caatgtagac ttgccaggag
     1681 ttaaaaagaa cgaaataaag ttgaatgtaa ccgatactgc aatagaagta tctgcagagc
     1741 acaaagaaga agatgaagaa aagaagaaaa attacgtacg aaaagaacga aaggaagtat
     1801 catatcacag aacaatgtca ttgcctgaga aagtgatttc atcaaaagca acggctaaac
     1861 taaacaatgg tgttttgagt gttgaaattc ctaaagttac tccaacacta aagccaaaaa
     1921 gtacctcaat tccaattcaa taatttcctc tttaattttt taaattttta tgttgttttg
     1981 tacaacccta gaagaatcaa caagagcatg caattctgat ttatccagtt caatttcgtt
     2041 tattttatca acaccatttt ttcctattat aactggtaca ccaagacaga cattagacaa
     2101 accatattca ccatctaaaa gtattgaagc tggaacttgt agtttttcgt cataaatgat
     2161 tgctttgatc aagtcaaatg tgttttttgc agcaccaaaa actgaagcat ccttgaatgt
     2221 tttaagcaat atccaataat ttcgtagttc cgttgttatt ttttgaactt tgatttgatc
     2281 taacatattt gatataggtt ttccatcata tctagcagaa gaaaatattg gaaccatcga
     2341 gtctccatgt tcacctatga ctattccttc tatttttgat tggtttactc caagcgtttt
     2401 tgcaagcaag tatctaaatc tactagaatc cagactagat cccattccaa taacattttt
     2461 tcgtgaaaat tgtgtttcct tcaaagtaaa ataagtgatg acgtcaagtg gatttgtcac
     2521 cacaatgatc ttagtgtcat cattatgttt tttgatgtta tttacaattt cccgtattaa
     2581 tgaaacgtta tctgcaagta aatcggttct tgatacagca atttttcctc tacttgcagc
     2641 aattacaatc actttggaat ctcttgttaa ttcataatca tcagttcctg taaccgatat
     2701 ggttgaacct ttaggaacag catttgtgat atcaagtgct tcgcctattg ctctgttctt
     2761 gttatgattt actaatacaa gatcatcaag tgaagttgat gcaataagaa aacctatggc
     2821 tgaaccaacc ttacctgcac caataacaga gattatggaa tttcactact cacttgatta
     2881 tcagtacagg cattttcgat ttatgagcca cagcatttga aacactccca aggaactttt
     2941 cttttactga acccagtcca cgtgcaccaa taacaataag atcaaatcct ttatcgtgtg
     3001 caaaaccaac aatttcttcc gcttcatgtc catcagttat tttttcattg aaaacaatac
     3061 cattttgagc ggcgcgtttt tttgcctttc ctagaaattc tttgcctgcc tttgcaagtt
     3121 ctttttcata taaaatcggg gttaagatta atgattttgg ataaaaggga atcacatata
     3181 gaccagttaa tgttgcaccg cattgtcttg caagataaat ggcagtgtct aaccctcgaa
     3241 gtgaattctt tgaaccatcc ataggaacaa gaatcttatt tatttttggt aatcgcatag
     3301 aatgcgttat gcaagtctta atttattaat gtacttagaa tatcatttct gataatctct
     3361 taatttacgc atggatagtt caccgaagaa atcatcctaa ttatcataca cgattatcaa
     3421 tatcaataat cagtcttcat tttttataac tcacacgggg catcttcagt gtaaaaaatg
     3481 aattatatac caattaaaag agttgcaatg gaggtatact aatgaattca gatcaaagta
     3541 catacgaata tgtaaaagaa gtgcaaaaaa tgccaataag aaaggcatta cacccagcta
     3601 ccatagttga accatcattt tcaatttcca aagttatcga catgatgtct aaaactgact
     3661 cgtatgatgt atattgtatg aacgaaggca cagtattaac aactaatgca cgtgaaattc
     3721 tgacagttaa agatgctttc actatgaaaa tttcctcttt gttacacaag attcgagcat
     3781 tatctaaaga tgatacaata gaaaaagctg caactgca
//
LOCUS       DATIDL010000037         8388 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrosopumilaceae archaeon isolate SMAG_U16342
            k141_2066133, whole genome shotgun sequence.
ACCESSION   DATIDL010000037 DATIDL010000000
VERSION     DATIDL010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935836
            Sequence Read Archive: SRR25158326
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosopumilaceae archaeon (soil metagenome)
  ORGANISM  Nitrosopumilaceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrosopumilales;
            Nitrosopumilaceae.
REFERENCE   1  (bases 1 to 8388)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8388)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/04/2024 05:09:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,520
            CDSs (total)                 :: 1,474
            Genes (coding)               :: 1,474
            CDSs (with protein)          :: 1,474
            Genes (RNA)                  :: 46
            rRNAs                        :: 1 (5S)
            complete rRNAs               :: 1 (5S)
            tRNAs                        :: 43
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8388
                     /organism="Nitrosopumilaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2066133"
                     /isolate="SMAG_U16342"
                     /isolation_source="soil"
                     /db_xref="taxon:2202732"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..527)
                     /locus_tag="VLB45_01665"
     CDS             complement(<1..527)
                     /locus_tag="VLB45_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016939435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HSD04445.1"
                     /translation="MLKEHNSWFANSIPLIASENVPSPAVREATISDFANRYAEGWPG
                     ERVYAGCTFIDKVEFKCIDLAKKLFKAEFVDVRPISGVVANLSVYSAFTNPGDVMLAP
                     SIPSGGHISHGKKEHSGTAGLVHGLDIEFYPFDAEEMSIDVDKTKQKVVELVKAGKTP
                     KMAMFGGSVFLFPHPV"
     gene            709..2409
                     /gene="thsB"
                     /locus_tag="VLB45_01670"
     CDS             709..2409
                     /gene="thsB"
                     /locus_tag="VLB45_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014966073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thermosome subunit beta"
                     /protein_id="HSD04446.1"
                     /translation="MSMQNAQGGIPVVILKDGATQTKGRDAQKNNITAAKLIAEIVHT
                     SLGPRGMDKMLVDSLGDVTITNDGATILKEIDVQHPAAKMLVEISKATDNEVGDGTTS
                     AVVLAGALLENAESLIQQEVHPTIIVDGYRKAAQKTIQLLEKMADKVLPTDKEVLNKI
                     ARTSMQTKLVRKDSGGLAEMVVKAVLAVAEKSGEEYKVDIDDIKVEKKAGGSIKDTLY
                     TKGIILDKEVVHSGMPKKIINAKIALINSALEIDKTEFDAKINISSPEQMKSFLDEEN
                     KMIKNMVDKVIASGANVLFCQKGIDDVAQHYLAKAGILTVRRIKESDLTKLAKATGAR
                     MVTNLDDLFDKDLGSAENVEERKVETDRWVFVEGCKHPKAVTLLIRGGSQRVVDEVER
                     SMHDALMVVKDVMENPMIVAGGGAPETYAATKLREWSKSLEGREQLAVEKFADSLEVI
                     PLTLAENAGMDPIDTLTSLRSKQLKGNKWTGIDVMKGVVASMHSTDIFEPLSVKKQIV
                     SSATEATCMILRIDDVIAIAKSAGPPPGAEGMGGMGGMGGMGGMGGMPGMGGMPPMDM
                     "
     gene            2475..2756
                     /locus_tag="VLB45_01675"
     CDS             2475..2756
                     /locus_tag="VLB45_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012216173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04447.1"
                     /translation="MQKGGSKILAGVKYVYLIFFFALLSGMFYPIIENGANANQVAIG
                     VMILFVGLAGALCLYKAGTSEKRQRTYLIAGFAILSIALLLVFSAAGRL"
     gene            complement(2758..4218)
                     /gene="glnA"
                     /locus_tag="VLB45_01680"
     CDS             complement(2758..4218)
                     /gene="glnA"
                     /locus_tag="VLB45_01680"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008297089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HSD04448.1"
                     /translation="MPYKVIGGEPTSISYSSDEVFAKIKHEQIKFIDLQFTGLTGRFH
                     HTTISSNLFTQQQMQDGLPKLDGSSIVGFAHVDDSDLILKPDPNTYAPIPWIANGKTA
                     RLICDIYWGWDKGRLESDPRSIAQKAEKYVKTQGFDFSLWGPEVEFFVFDKVSWDVLT
                     PYKGQSYSIESKEAPWSQEGTGYPMGLQEGYYPSTPSDTLTEFRNDCVECLNEDFGIL
                     CDNHHHEVATAGQCEIDIRYDYLTNAADGAQTYKYVVRNVAQKYGKVATMMPKPISMD
                     AGSGMHTNVSLWKNDKNIFFDKDDKEEISQTARYFCGGIMNHARALAALVAPTTNSYH
                     RLVPGYEAPVYIAWSSSNRSAIVRVPSHFKGEKYSKIKRLEFRAPDPSSNPYLVFAAV
                     LSAGLDGVKKKIEPGDPVHEDIYKMNKDERAKRGIKSLPATLGEALDSLESDRKFLNP
                     IFTNEVIDKLIELERKDEREVSIRPHPHEFYLYFDI"
     gene            4277..4834
                     /locus_tag="VLB45_01685"
     CDS             4277..4834
                     /locus_tag="VLB45_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04449.1"
                     /translation="MSEIKKIDVNSLYGILLREIENDSIQEIDSHLYTNISDFLGNLK
                     NQGYDGIESKIKDALVKMITDMVSLLLKIRIEKAVKSKELDYSSLLDEERYILDSQDE
                     LLQRKDTVLAATLNGRMKLLETLAKNHKSRAIVVRFLKPIDQIIGSDLEKYGPFEAED
                     VATLPFENALALIGKKIAVKISWED"
     gene            4866..5141
                     /locus_tag="VLB45_01690"
     CDS             4866..5141
                     /locus_tag="VLB45_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008297136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04450.1"
                     /translation="MSHLGIDIDFYFLLTVYPVVGLFVVEIICRVAKIKSWIKLTTQA
                     IMCVGFGIAYVTMIVSHWITSIVLIALAGALFYQARIAKVKPDQSTY"
     gene            5213..5953
                     /locus_tag="VLB45_01695"
     CDS             5213..5953
                     /locus_tag="VLB45_01695"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HSD04451.1"
                     /translation="MIKDKVAIVTGASSGIGYATAVALSKAGAKVAVAARRTELLEQL
                     QNEIKKNGGECLVVSCDVTKRKDCYALIDATMKKWNRIDILINNAGIMPLSFVKSLKV
                     DEWEQMVDVNIKGVLFCTAAAIPHMVSQNSGHIVNLSSIAGRVVFAAGSVYCATKYAV
                     RAFSEGLRQELSTKHNIRVTTIEPGIVATELTNTITEKSLESFLKYTKSIDALKSEDI
                     ANAIMFAVDSPPHVNVNEIMVRPTQQER"
     gene            5967..7115
                     /locus_tag="VLB45_01700"
     CDS             5967..7115
                     /locus_tag="VLB45_01700"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014966066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HSD04452.1"
                     /translation="MFIITKNILILGGGFGGLAAANELRQNLPADIKITIIDKKDYFM
                     MDLVKLWILNGTREFEFSKRPLQPITKKGIDFINEEITKIDTKKKTVTTKSKELSYDH
                     LIIALGVELAPEQISGLSENGLVLYDIEDVPKIRDAIKNIKSGKIVMAIMGMPYKCPP
                     APFEAALLISSMLRDLGTSNSIQIEFYSPAPITLPAAGPQVSEELLQLLKSRNIAFHG
                     SHKTVSVGANTLKFENSETQFDLLIAVPPHKAPTVVVDSGFAQKGKFVSVSRNCKTNF
                     ENVYAIGDVNEMMVTDKIAVPKAGIFAEGEGVAVAKNIISQIRQEQEKAIFDGKGGCF
                     VEMGKQTAGYLQVDMFASPNPVTILKEPTQENFVDKEKFEKDRLAQWL"
     gene            complement(7110..7310)
                     /locus_tag="VLB45_01705"
     CDS             complement(7110..7310)
                     /locus_tag="VLB45_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04453.1"
                     /translation="MKICSICKKISASEEDHLDCMEKRRIELDAEDFKESIAERLTIG
                     DSNELHADIKGILEHLGKKKKS"
     gene            7393..7467
                     /locus_tag="VLB45_01710"
     tRNA            7393..7467
                     /locus_tag="VLB45_01710"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7428..7430,aa:Pro,seq:ggg)
     gene            7558..7716
                     /locus_tag="VLB45_01715"
     CDS             7558..7716
                     /locus_tag="VLB45_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007550289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04454.1"
                     /translation="MKITMDDTNPTDKPKKDRMVHASFRKVVAFDTLFFVLGVLTGIG
                     IGVYLIAN"
     gene            complement(7730..8338)
                     /locus_tag="VLB45_01720"
     CDS             complement(7730..8338)
                     /locus_tag="VLB45_01720"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HSD04455.1"
                     /translation="MKGQKAIRFLKQDPRLSEIIKQVGSYQIKQRNHHFASLVEAIIS
                     QQLAGAAADAILQRFKKLYPAFPKPADVLDTKDSELRLVGLSKMKIEYLKDLAKKIER
                     GEIKIKTLSKMTDEEVISHLTQVKGIGRWTAEMFLIFSLGRQDVFPVGDLGLRKGVQK
                     AFSLKELPNPKDVEEIGMRWKPYRSIATWYLWKSLQNFDNIG"
BASE COUNT         2758 a         1510 c         1608 g         2512 t
ORIGIN      
        1 acaggatgag gaaacaaaaa gaccgaacct ccaaacattg ccatcttggg agtcttgcca
       61 gcttttacaa gctcgactac tttttgtttt gtcttatcca catcaattga catttcctct
      121 gcatcaaacg gataaaattc aatatctaat ccatgtacta gacctgctgt tcctgagtgt
      181 tctttttttc catgagatat atggcctcca gatggaattg atggtgcaag catgacatca
      241 cctgggtttg taaaggcaga ataaactgaa agattagcta caactccaga aattggtctt
      301 acatctacaa actctgcttt gaataatttt tttgcaagat caatgcattt gaactcgact
      361 ttatcaataa aggtgcaacc ggcatagaca cgttctcctg gccagccttc tgcgtatcta
      421 tttgcaaaat cagatattgt tgcctctctt actgcaggac tggggacatt ttcacttgca
      481 atgagaggaa tggaatttgc aaaccatgaa ttgtgctctt ttagcataga aaacacttct
      541 ttgtaagatt ccctaggaga agatcgcgtc atattttggg ggactaatta tcctaattta
      601 aaaacatcgg attgagattt ttgaaaaatc atctgtttaa cgaaattagc caattctagt
      661 gacttacaaa taaagacata aaaagggaaa aacgggctct actaaaacat gtcaatgcag
      721 aacgctcagg gaggaattcc tgttgttata ctaaaagatg gtgcaacgca aactaagggt
      781 cgcgatgcac aaaaaaataa cataacggca gcaaaattga ttgccgaaat tgtgcacaca
      841 agtcttggac caagaggcat ggacaagatg cttgtggatt ctctaggtga tgttacaatt
      901 acaaacgatg gagcaacaat tctcaaagaa attgatgtgc aacacccagc tgcaaaaatg
      961 ctagttgaaa tttccaaggc aactgacaat gaagtaggag atggtactac atcagctgta
     1021 gtccttgctg gggcactttt agaaaatgct gaatctctaa tacaacaaga agttcatcca
     1081 accatcatag tagatggata tagaaaagct gcccaaaaaa caattcaact cttggaaaaa
     1141 atggcagata aagtattacc aactgataaa gaagttctca ataaaattgc aagaacctca
     1201 atgcagacaa aacttgttag aaaagattct ggcggtctgg ctgagatggt ggtaaaggct
     1261 gtacttgcag tagcagaaaa atctggcgag gaatacaaag tcgatataga tgatatcaaa
     1321 gttgagaaga aagcaggcgg ttcaataaag gacacattgt acacaaaagg aatcatactt
     1381 gacaaagagg ttgttcactc tggtatgcca aaaaaaatta tcaatgcaaa aattgctctc
     1441 attaactctg ctttagaaat tgacaaaact gaatttgatg caaagatcaa catatcaagt
     1501 cctgaacaaa tgaagtcatt tctggatgaa gaaaataaga tgatcaaaaa catggttgac
     1561 aaagtcattg catcaggtgc aaatgtgttg ttttgtcaaa aaggaattga tgatgttgca
     1621 caacattatc ttgcaaaggc tggcatactt acagtaagaa gaataaaaga aagtgatctt
     1681 accaagcttg caaaagcaac tggtgcaaga atggtaacaa accttgatga tttgtttgat
     1741 aaagacttgg gttctgcaga aaacgtcgag gaaagaaaag ttgaaacaga caggtgggtc
     1801 tttgtagaag gctgcaaaca tccaaaggca gtaactctac tcatacgtgg aggctctcag
     1861 agagttgttg atgaagttga aagatcaatg catgacgcac taatggtagt aaaagatgtg
     1921 atggaaaacc caatgattgt tgcaggtgga ggtgcaccag aaacttatgc agcaacaaaa
     1981 cttagagaat ggtcaaaatc acttgaggga cgagaacaac ttgccgtaga aaaatttgca
     2041 gactcacttg aggtgatacc tcttacacta gctgaaaatg ctggaatgga tccaattgac
     2101 acactaacat cacttcgttc aaaacaattg aaaggaaaca agtggactgg aattgatgta
     2161 atgaaaggtg ttgttgcaag catgcattct actgacatct ttgaaccact atcagtcaaa
     2221 aaacaaattg tttcgtctgc tactgaagca acatgtatga ttcttagaat tgatgatgta
     2281 attgcaattg caaaatctgc aggtccacca ccgggtgctg aaggaatggg cggcatggga
     2341 ggaatgggtg gtatgggcgg catgggtggt atgcccggaa tgggcggaat gccacctatg
     2401 gacatgtaag tttacaaaca agtttctaaa acaatccatc aaggtttatt tgcagagttt
     2461 tgttacgaga ttttatgcaa aaaggcggct ccaaaattct tgctggagta aagtatgtgt
     2521 atttgatatt tttctttgca ctgctttctg gaatgtttta cccaataatt gaaaacggtg
     2581 caaatgcaaa ccaagttgct attggtgtaa tgattctctt tgttggtttg gctggtgcat
     2641 tatgtctata caaagctggc acttcagaaa aaagacaaag aacgtatcta atagctggat
     2701 ttgcaatttt atcaattgca ttattacttg tattttcagc agccggaaga ttgtagatta
     2761 aatatcaaaa tacagataga attcatgtgg atgaggtcgt attgatactt ctctctcatc
     2821 ttttctttct aactctatga gtttgtctat cacttcattt gtaaatatgg ggttgagaaa
     2881 ctttctgtca ctttcaagcg aatctaatgc ttcaccaagt gttgcaggta gtgatttgat
     2941 tcctcttttt gcacgttcat ccttattcat cttgtaaata tcctcatgga ctggatcgcc
     3001 tggttctatc ttcttcttga ccccatcaag ccctgcagaa agaacagctg caaatacaag
     3061 atagggattg gaagatgggt ctggggctct gaactctaat cgctttattt ttgaatattt
     3121 ttctccttta aagtgggatg gaactcttac aattgcagat ctgttacttg agctccatgc
     3181 aatatagact ggagcttcat aacccggaac cagcctatga tatgagtttg tggttggtgc
     3241 tacaagtgca gctaaagctc tagcatgatt cattatacct ccacaaaagt atcttgcagt
     3301 ctggctgatc tcttctttgt cgtctttatc aaagaaaata tttttgtcat ttttccacaa
     3361 actgacattt gtatgcattc cagatcctgc atccattgaa attggttttg gcatcattgt
     3421 tgcaaccttg ccatattttt gtgcaacatt tctcactaca tacttgtagg tttgtgcacc
     3481 atcagctgcg tttgttagat aatcatatcg tatatcaatt tcacattgac ctgcagttgc
     3541 gacttcatga tgatggttat cacataatat gccaaaatct tcattcagac attctacaca
     3601 atcatttcta aattctgtca atgtatctga tggagtactt ggataatatc cctcctgtag
     3661 acccatggga tatccagttc cttcttggct ccatggtgcc tctttagatt ctattgagta
     3721 tgattgacct ttgtatggtg tcaagacatc ccaagaaact ttatcaaaaa caaaaaactc
     3781 cacctcaggt ccccataagc taaaatcaaa tccttgggtt ttgacgtatt tttctgcctt
     3841 ttgcgctatt gatcttggat cactttcaag tctacctttg tcccatcccc aatagatatc
     3901 acaaattaat ctggcagtct tgccgtttgc tatccaagga ataggtgcat aggtgtttgg
     3961 atctggtttt aatatcaaat cagagtcgtc tacatgcgca aatccaacaa ttgaagagcc
     4021 atcaagttta ggcaatccat cttgcatttg ttgttgggta aacagattag atgaaattgt
     4081 tgtatgatgg aatctaccag ttagtcccgt gaattgtaaa tcgatgaatt ttatctgttc
     4141 atgttttatc tttgcaaaaa cctcatcaga tgaataagaa attgaagttg gttctccacc
     4201 aatcacttta tatggcaact ttgcaccttg atcataacac aaggagtctg ccatttaagg
     4261 attattgggt gtgtaaatgt ctgaaataaa aaaaattgac gttaattcat tgtacggaat
     4321 tttgctacga gaaatcgaaa atgattcgat ccaagaaatt gattcgcatc tttatacaaa
     4381 catatctgat tttcttggta atctaaaaaa tcaaggatat gatggaattg aatcgaaaat
     4441 aaaagatgca ttagttaaaa tgataactga tatggtatct cttcttttga aaataagaat
     4501 tgaaaaagca gtaaaatcaa aagagttgga ttattctagt ttgcttgatg aggaaagata
     4561 tattctggat tctcaagacg aactacttca gagaaaagat actgttcttg cagcaacact
     4621 aaacggtaga atgaagcttc ttgagacact agcaaaaaat cataaatcaa gagctatcgt
     4681 ggttcgattt ctaaaaccaa tagaccaaat catagggtca gatcttgaga agtatggtcc
     4741 atttgaagct gaggacgtgg caacacttcc atttgaaaat gcgcttgctc tcattggcaa
     4801 gaaaattgcc gtaaaaataa gctgggaaga ctagaaatta cgtaaactat tttcaaagat
     4861 aggaaatgtc ccatttagga atagatattg atttttactt tttactcaca gtttatccgg
     4921 ttgttggact ttttgttgta gaaatcatct gtagagtggc aaaaatcaaa tcttggataa
     4981 aactcacgac tcaggcaata atgtgtgtag gatttggcat tgcatacgta actatgatag
     5041 tgtcacattg gattacctcc attgttctaa tagctttggc aggtgcacta ttttatcaag
     5101 caagaattgc caaagtaaaa cccgatcaat caacttacta gcaaatcgga atacaagaat
     5161 ttcaacaaaa catatttaca caatatccag tgataaacaa cagattttca atatgattaa
     5221 agataaagta gcaattgtaa caggtgcaag cagtggtatt ggttatgcga ctgcagtggc
     5281 tttgtcaaaa gcaggagcaa aagtggcagt tgctgcaaga agaactgaac tactggaaca
     5341 attacaaaat gaaattaaaa aaaatggcgg ggaatgtctt gttgtttcat gtgatgtaac
     5401 aaagcgaaaa gactgttatg cattaattga tgcaaccatg aaaaaatgga atcgtattga
     5461 tattttaatc aacaatgctg gaataatgcc actaagcttt gtcaaaagtc tgaaagtcga
     5521 tgagtgggaa caaatggttg atgtaaacat aaagggtgtg ctgttctgca ctgctgcagc
     5581 aattccacac atggttagcc aaaattctgg tcatatagta aatctctcat ctattgcagg
     5641 gagagttgtt ttcgctgcag gaagtgttta ttgtgcaaca aaatatgctg tgagagcatt
     5701 tagcgagggc ctaagacaag aactcagcac gaaacataac atccgagtta caaccattga
     5761 accaggaatt gtggcaactg agctcacaaa tacaattacg gaaaaatcct tggaatcatt
     5821 tctcaaatac acaaaaagca ttgatgctct caaatctgaa gatatagcaa atgccataat
     5881 gtttgcagtt gattcgccac cacatgttaa tgtcaacgaa atcatggtaa ggccaactca
     5941 acaagaaaga taattctgat tggtgtttgt ttattataac caaaaacatt ctgattcttg
     6001 gtggtggatt tggaggtctt gcagctgcaa atgagctaag acaaaatctt ccagcagaca
     6061 tcaaaataac aataattgat aaaaaagact atttcatgat ggatctagta aaactctgga
     6121 tcctaaacgg cacgagagaa tttgaatttt caaaaagacc actacaacca ataacaaaga
     6181 aaggaattga tttcataaac gaagagataa ccaagataga cacaaaaaag aagactgtta
     6241 caacaaaatc aaaggaatta tcttatgatc atctaataat tgcacttggt gtagaactgg
     6301 caccagaaca aatttcaggt ctatctgaaa atggacttgt tttatatgat attgaagacg
     6361 ttccaaaaat tcgagatgcc atcaagaaca tcaaatctgg caaaattgta atggcaataa
     6421 tggggatgcc atacaaatgc cctccagccc cgtttgaagc agcacttctc atcagctcga
     6481 tgttacgaga tcttggtact agtaactcga tacaaatcga attttacagc ccagcaccaa
     6541 taactctgcc tgccgcagga cctcaagtaa gcgaagaact attgcaactt ttaaaatcta
     6601 gaaacatcgc ttttcatggc tctcacaaaa ctgtctctgt tggagcaaat actctcaagt
     6661 ttgaaaactc tgagacccag ttcgatcttc tcatagcagt tcctccgcac aaagctccta
     6721 cagttgtagt agactcgggg tttgcacaaa aaggcaagtt cgtatcagtt agtagaaact
     6781 gtaaaacaaa ttttgaaaat gtctatgcga taggtgatgt aaatgaaatg atggtcacag
     6841 acaagattgc agttccaaaa gcaggaattt ttgcagaagg tgaaggggtt gcagttgcaa
     6901 aaaacataat ttcacaaatt agacaagaac aagaaaaagc aatctttgac ggaaaaggtg
     6961 ggtgctttgt tgaaatggga aaacaaacag caggatatct tcaagttgac atgtttgcca
     7021 gtcctaatcc tgttactata ctaaaagaac ctacacaaga aaactttgtt gataaagaga
     7081 aatttgaaaa agacagactt gctcaatggt tatgatttct tcttttttcc taaatgctct
     7141 aaaattccct taatgtcagc gtgaagttca ttactatcac ctattgtcaa tctctctgca
     7201 attgattctt taaaatcttc agcatcaagt tctattcttc ttttttccat acagtcaaga
     7261 tgatcttcct cacttgctga gattttttta caaatggaac agatcttcaa tgtattagtg
     7321 acgtatacac ttgattttaa tcattcgagc tgaacccacg atttaataat ttcactattg
     7381 ccagcaactt gaggggccgt agtctagctt ggtcatgatg ctagcctggg gtgctagagg
     7441 tcgccggttc aagtccggcc ggccccatta cataatacta gtaatttaac aatgcggata
     7501 aaaaatcata atcaaaattg aacaaaggtt tttcatatac acatactata ccaactgttg
     7561 aaaataacta tggacgatac aaaccctaca gacaaaccaa aaaaagacag aatggttcat
     7621 gcctccttta gaaaagtagt agcatttgat actctatttt ttgtattggg ggtattgaca
     7681 ggaataggaa ttggcgttta tctgatagcc aattaaactc catttttgtt taaccaatat
     7741 tatcaaaatt ctgcaaactc ttccacaaat accatgttgc tatgctacga tatggtttcc
     7801 atctcatccc tatctcttca acatcttttg ggtttggtaa ttcctttagt gaaaaggcct
     7861 tttgcacacc ttttcttaac ccaagatctc ctactggaaa gacatcttgt cttcccaaag
     7921 aaaaaattag aaacatttct gctgtccatc tccctattcc cttaacttgt gtaagatgtg
     7981 atattacttc ctcatcagtc atctttgaca aggttttgat tttaatttca ccacgttcta
     8041 ttttctttgc aagatctttt aggtactcta ttttcatttt cgataatcca accaaacgta
     8101 attctgaatc ttttgtatcc aaaacgtcag caggttttgg aaatgcagga tatagtttct
     8161 tgaatctttg tagtatggca tcagctgcag cacctgcaag ttgctgtgaa ataatggctt
     8221 ctactaatga agcaaagtgg tggtttcgtt gttttatttg ataactgcca acttgcttaa
     8281 tgatttcaga aagacgtgga tcctgtttta gaaaacgaat tgccttttgc cctttcattg
     8341 tctagtaggt tctggatgaa ttgttattat agcattttta atttggtt
//
LOCUS       DATIDL010000038         4998 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrosopumilaceae archaeon isolate SMAG_U16342
            k141_1261116, whole genome shotgun sequence.
ACCESSION   DATIDL010000038 DATIDL010000000
VERSION     DATIDL010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935836
            Sequence Read Archive: SRR25158326
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosopumilaceae archaeon (soil metagenome)
  ORGANISM  Nitrosopumilaceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrosopumilales;
            Nitrosopumilaceae.
REFERENCE   1  (bases 1 to 4998)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4998)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/04/2024 05:09:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,520
            CDSs (total)                 :: 1,474
            Genes (coding)               :: 1,474
            CDSs (with protein)          :: 1,474
            Genes (RNA)                  :: 46
            rRNAs                        :: 1 (5S)
            complete rRNAs               :: 1 (5S)
            tRNAs                        :: 43
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4998
                     /organism="Nitrosopumilaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1261116"
                     /isolate="SMAG_U16342"
                     /isolation_source="soil"
                     /db_xref="taxon:2202732"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..235
                     /locus_tag="VLB45_01725"
     CDS             <1..235
                     /locus_tag="VLB45_01725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04456.1"
                     /translation="QGNFIKFDLDEYDGNEHDTNFFRSHSVTNPVLITFDDSTSVEKI
                     DNFISNLKSRNIVFVNVSELTGIELTIRQGVKN"
     gene            232..1674
                     /locus_tag="VLB45_01730"
     CDS             232..1674
                     /locus_tag="VLB45_01730"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HSD04457.1"
                     /translation="MTLELENYLHKNIQNQKCGQQISKSGIIKISKKAWMTRICILTL
                     ASLVVAYNIQLGIELEEPLLIYANLLPLHGILYLTVGWFFYRNPAKGKMGNELVSVII
                     PVYNQKAMIEIVIDAIYNSTYKNIEVIAVNDGSTDGSKEILDKLAKKYPTLTVIHKEN
                     EGKRKAVAISFYRSKGKYLILVDSDSVIDKHAIAEMMKTFHGDPKIGATVGFAKAWNA
                     EKNILTRCQDVWYDYSFNIRKACESVFGSVMCCSGCLAGYRREAIAHYIPYWVRSKIH
                     DSEDRELTTYVVATPWAKSEFKKHYAGLPSFSQGTLESMAQYDDAEDRGLTAQVLHAW
                     KAVYVASAIVYTDVPEKLRGFIKQQQRWKKGTTRVNFFVGSFFWRKHPIMSLIFYLDF
                     MMAFITPFIVMTVFLYIPLVHQSWWHPLVYLAGMQLTSIAHGSDYKFRDSNAKNWKYK
                     PVINILTAFVTSWLIFPAIWNYKKNQWLTR"
     gene            1729..2625
                     /locus_tag="VLB45_01735"
     CDS             1729..2625
                     /locus_tag="VLB45_01735"
                     /inference="COORDINATES: protein motif:HMM:NF021601.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HSD04458.1"
                     /translation="MAASIQESFAITSCNCVAFRLDDIQNYFLNNAQVEIINTFQKNN
                     ANLTVGVIGNYFGEDPVITNFLKERLENGTPKIEIANHGWNHENFSEYDKAQQHVLIL
                     KTNEKISSMLGVMPIGFITPFNALNDDTLSALKENKMVYISANKTADPPPYALSNTEL
                     YRLPSVAYVGNFNNDDTDWVTKTHKQTHVQILDGLLNYGYAIVTMHPQDFVKRDKLSY
                     SNEVDWNQIHELELLIDAIRNDGLKIVTISDIPQHANVHQKTPEWIKTIFDWYEDDKI
                     SADEVLNAIKYLKEKKILQLDI"
     gene            2753..4123
                     /locus_tag="VLB45_01740"
     CDS             2753..4123
                     /locus_tag="VLB45_01740"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HSD04459.1"
                     /translation="MATYLIIVSMAFAGAIIFYQLSSARGDLSSKFKYQKKTMSPFAI
                     IMVALPVVLAFVSAYEGYSIWESPNRIIILWGMTITFWSTLMFVPLAVYSKYREDMTP
                     DPARYPSVSVLVPAYNEEKVIARTIEGLIDTDYPNKEVIVIDDGSKDRTLEISNRYKS
                     QVKVLHKENGGKASALNYGLAFSKGEIIVIVDADTIIGRNALKQVVKGFEGDDNVAAV
                     AGNIKVRNRMNWLTWCQALEYVAGIEIIRRAFDFFGSITIVPGALGAFRRTSLEEAGS
                     YHKDTLVEDFDATIKILKSGFVIQGSTSATAYTEAPQTLHDFYNQRKRWYRGNIQVLS
                     RHMEALFNPRYAFLYKLAFPFMIISMIILPFAGLSVIITSIIEIIYGDGLFVLVLFLL
                     FMLLQCLQASLAVRIDRDDPKMILFSPFLVLGYKQLVDMLLIKSTIEALFKRKAVWTS
                     AKRVGV"
     gene            4128..>4998
                     /locus_tag="VLB45_01745"
     CDS             4128..>4998
                     /locus_tag="VLB45_01745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04460.1"
                     /translation="MIDIKIKLWQKFLLIAIILVLQPILVHAEESTGSVEINVKYTNG
                     DRADEYGMVLKIYQDFNRTPYKEIESVSSNPYNITLPLGHKYTVEVYVNSMYSSVGYI
                     NLQKIQERLDISIPLSSGLRFNVFFKDGTTPMPGATVIVKSQDGKQWGERTTDDLGQT
                     QRLWIQSTTKDSDYYVADIMLSDDIKYTYSPIKLTAGEKYESKIVTPWPAIIDELITV
                     NIYKNTYTKIGPSDGKFIVELYNGNENKVTESLVNAKGEAHFSKIKVGTYTFAVMKLE
                     NNVRQEWNIKKITI"
BASE COUNT         1734 a          850 c          945 g         1469 t
ORIGIN      
        1 tcaaggaaat ttcattaaat ttgatcttga tgaatatgat ggtaatgagc atgataccaa
       61 cttctttcgt tctcattcag taactaatcc agtactcatt acttttgatg attctacatc
      121 agtggagaaa attgacaatt ttatttcgaa tctaaaatct cgtaacattg tttttgtaaa
      181 tgtatctgag ctaactggaa ttgaattaac cattcgccaa ggagtgaaaa attgacatta
      241 gagcttgaaa attatcttca caaaaatatt cagaaccaaa aatgcggtca gcagatatca
      301 aaaagtggta ttattaaaat cagcaaaaag gcatggatga caagaatctg catactcaca
      361 cttgccagtc tagttgtggc atacaacata caattaggca ttgagttaga agaacctctt
      421 ttaatttatg caaatctact tcctctacat ggcatattgt atttaacagt gggctggttc
      481 ttttacagaa atcctgcaaa gggtaaaatg ggtaacgaat tggtatcggt aataattcca
      541 gtatacaatc aaaaggctat gattgagata gtaatagatg ccatctacaa ttctacttat
      601 aaaaacatcg aagtaatcgc tgtaaatgat ggcagcactg atggaagcaa agaaattctg
      661 gacaaactag cgaaaaaata tcccacttta acagtgattc ataaggaaaa tgaaggaaaa
      721 cgaaaggcag tagctatttc cttttacagg tcaaaaggca agtatctcat acttgtagat
      781 tctgacagtg tgatagacaa acatgccata gctgagatga tgaaaacatt tcacggagat
      841 ccaaagattg gcgctactgt tggatttgcc aaagcatgga atgctgaaaa aaacatactc
      901 accaggtgtc aagatgtttg gtatgactat tcctttaaca tacgaaaagc ttgtgaaagt
      961 gtatttggct ctgtaatgtg ttgctctgga tgtcttgctg ggtacagaag ggaagcaata
     1021 gctcattata ttccatattg ggtgcgttct aaaattcatg atagtgagga tagagagctt
     1081 actacgtatg ttgttgccac tccatgggcc aaatctgaat ttaaaaaaca ctatgcagga
     1141 ttaccatctt tttcacaggg gacgttggaa tccatggcac agtatgacga tgcagaagat
     1201 cgtggcctga cagctcaagt tcttcatgca tggaaggcag tatatgttgc aagtgcaatc
     1261 gtatatactg acgtaccaga aaagctcaga gggtttatca aacaacaaca aagatggaaa
     1321 aagggtacca ccagagtaaa tttctttgtt ggctcatttt tttggcgtaa acatccaata
     1381 atgtcgttga tattttatct tgattttatg atggcattca taacaccatt tatagttatg
     1441 acagttttct tatacattcc acttgtacat cagtcttggt ggcatccctt ggtttatctt
     1501 gctgggatgc aattaacatc tatcgcacat ggctcagatt ataaattccg tgattcaaat
     1561 gcaaaaaatt ggaagtacaa accagtcata aacattctca cagcttttgt gacatcatgg
     1621 ttgatatttc cagcaatatg gaattacaag aaaaaccagt ggttaacacg atgaacatgg
     1681 atgtatttta atttctttac aaaaatttct atatttgtta tactaatctt ggcagcatcc
     1741 attcaagaat catttgcgat tacttcatgc aattgtgttg cctttagact agatgacata
     1801 caaaattatt ttctaaataa tgcccaagtt gaaatcataa atacatttca aaaaaataat
     1861 gccaatctaa cggtaggagt tattggaaat tattttggcg aagatccagt tataactaat
     1921 tttctaaagg aaaggttaga aaacggtacg ccaaaaatcg agattgcgaa ccacggctgg
     1981 aatcatgaaa atttttccga gtatgacaaa gcccaacaac acgtgttgat tctaaaaaca
     2041 aacgaaaaga tatcaagtat gttaggtgtg atgcctattg ggtttatcac tccatttaat
     2101 gcgctaaatg atgatacatt atctgcctta aaagaaaaca aaatggtata cattagtgcc
     2161 aacaaaaccg cagatccacc tccttatgct ctctcaaaca ctgaacttta cagactgcca
     2221 tcagttgcat atgttggtaa ttttaacaat gacgatacgg attgggttac caaaactcat
     2281 aaacaaacac atgtgcaaat tttggacggt ctactaaatt acggatacgc catagtgaca
     2341 atgcatccac aggattttgt gaaaagagac aagttgagtt actcaaacga agtggattgg
     2401 aatcagattc atgagttgga actcttgatt gatgctataa gaaatgatgg cttgaaaata
     2461 gtgacaattt ctgacatacc tcaacatgca aatgtacatc aaaaaactcc agaatggatt
     2521 aaaaccatat ttgactggta tgaagatgat aaaatatcag cagatgaagt tcttaacgca
     2581 ataaaatatc tcaaagaaaa gaaaatatta caacttgaca tctaaagata tcattttact
     2641 accacatcaa agtatactac ctatgtctaa ttcacccaat agaagaattg atcgtgtcca
     2701 gatactcctt gtctgtacca tattcggagt gcttttcttt acggcatatc taatggctac
     2761 atatttgatc atagtttcaa tggcctttgc tggagccatt attttttatc agctttcaag
     2821 cgcaagagga gacttgtctt caaaattcaa gtatcagaaa aaaacaatgt ctccttttgc
     2881 aataattatg gttgcgcttc ccgtagtact ggcatttgtt tcagcttatg aaggatactc
     2941 tatttgggaa tcaccaaaca gaatcataat tttgtgggga atgacaataa cattctggag
     3001 tactttgatg tttgttccac tagcggtgta tagcaagtat agagaagaca tgactccaga
     3061 tcctgccaga tacccatcag tgagtgtact tgtcccagca tataatgaag aaaaggtgat
     3121 tgcaagaacg attgaaggtt tgatcgacac ggattatcct aacaaggaag taatagtcat
     3181 cgatgatgga agtaaggata gaactttaga gatttctaac agatacaaat cccaagtaaa
     3241 ggtattgcat aaagaaaatg gcggaaaggc atctgcacta aactatggac tagcattttc
     3301 aaagggcgag attattgtaa tcgttgatgc agataccata atcggaagaa atgcattaaa
     3361 gcaagtagtc aaagggtttg aaggagatga caacgttgcc gcagtggcag gaaacatcaa
     3421 agtcagaaac agaatgaact ggcttacttg gtgccaagcg ttagaatatg ttgcaggcat
     3481 agaaattatc agaagagcat tcgatttctt tggttctatc accatagttc ctggggcatt
     3541 gggagcattc aggagaacat ctctggaaga ggctgggagt tatcataaag atactcttgt
     3601 agaggatttt gatgctacta ttaagatttt aaaatcaggg tttgtaatac aaggaagtac
     3661 tagtgctaca gcatatacag aggcaccaca gacactgcat gacttttaca atcaaagaaa
     3721 acgctggtat cgtggaaaca tccaagttct ttcaagacat atggaggctt tgtttaatcc
     3781 acgttatgcc tttctataca agttagcatt tcctttcatg ataatttcaa tgattatttt
     3841 gccgtttgca ggattgtctg tcataataac atccataata gaaatcatct atggagatgg
     3901 tctctttgtg ttggttttat ttttactatt catgttatta caatgtctac aggcttcact
     3961 ggcagtacga attgataggg atgatccaaa gatgattttg ttttctccat tccttgtttt
     4021 aggatacaaa caactagtcg atatgctttt gatcaagtcc actatagaag cattgtttaa
     4081 aagaaaagct gtctggacca gcgcaaaaag agttggagta tgagatcatg atagatataa
     4141 aaataaaatt gtggcaaaaa tttttattaa ttgcaataat tctagtatta caaccaattc
     4201 tagttcatgc tgaagaaagt actggatctg tagagattaa tgtaaagtac acaaatggag
     4261 acagagctga tgaatatggg atggtcttaa aaatatatca agattttaac aggactcctt
     4321 acaaagaaat agaatcagta tcaagcaatc cgtacaacat aactttacct ttggggcaca
     4381 aatacactgt tgaagtctat gtcaatagca tgtattcaag tgtaggttat atcaatttac
     4441 aaaaaatcca agaaagactt gacatctcca ttccactttc tagcggactc cgtttcaacg
     4501 tttttttcaa ggatggaaca acacccatgc ctggtgctac agtaatagtc aagtcccaag
     4561 atggaaaaca gtggggagaa agaactacag atgatctggg tcagactcag cgtctgtgga
     4621 tccagtctac tacgaaagat agtgactatt atgttgcaga tatcatgctt agtgacgata
     4681 taaaatacac ttattctcca atcaaattaa ctgccggaga gaaatacgaa tccaaaattg
     4741 tcacaccttg gccagccata atagatgaac ttatcacagt aaatatctac aaaaacacat
     4801 acacaaaaat tggaccgtct gacggtaaat tcatagtaga actatacaat ggtaatgaaa
     4861 acaaagttac tgaatccctc gtcaatgcca aaggtgaagc acatttttca aagatcaaag
     4921 ttggtaccta tacttttgca gtaatgaaat tagaaaataa tgtccgtcaa gaatggaata
     4981 taaagaaaat aacaatca
//
LOCUS       DATIDL010000039         3931 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrosopumilaceae archaeon isolate SMAG_U16342
            k141_2676066, whole genome shotgun sequence.
ACCESSION   DATIDL010000039 DATIDL010000000
VERSION     DATIDL010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935836
            Sequence Read Archive: SRR25158326
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosopumilaceae archaeon (soil metagenome)
  ORGANISM  Nitrosopumilaceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrosopumilales;
            Nitrosopumilaceae.
REFERENCE   1  (bases 1 to 3931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/04/2024 05:09:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,520
            CDSs (total)                 :: 1,474
            Genes (coding)               :: 1,474
            CDSs (with protein)          :: 1,474
            Genes (RNA)                  :: 46
            rRNAs                        :: 1 (5S)
            complete rRNAs               :: 1 (5S)
            tRNAs                        :: 43
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3931
                     /organism="Nitrosopumilaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2676066"
                     /isolate="SMAG_U16342"
                     /isolation_source="soil"
                     /db_xref="taxon:2202732"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..471
                     /locus_tag="VLB45_01750"
     CDS             <1..471
                     /locus_tag="VLB45_01750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04461.1"
                     /translation="YPLLIIFSILYIYPLRKAISKKLVISTLTISIALLVPTFYTVYN
                     ANSEANEFEVALAASYPILDAIVLCPALIGVTLFFKGEVNFLWSLVCIGIVLNVVGDT
                     GFLILSIDESYYTGHPIDMLYLWAYTLFSFGVYSHIKIFKSHKKDTYENIDSLR"
     gene            complement(498..1493)
                     /locus_tag="VLB45_01755"
     CDS             complement(498..1493)
                     /locus_tag="VLB45_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007401252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HSD04462.1"
                     /translation="MPLPKISFIEKLTHSSSPRLGYVLVICAAALAGLIHSVSKPMLE
                     HSDVNLVEINPLTLAAVIFIINGLFFTPVKKNGDRVTKIGRKNIMLLLFIGIAEVSAI
                     ITYFFGLKESTAVNASILTNGEIIFSLLIAIVIFREKLQKKESVPFSLVIAGVVLLPL
                     GYDLYQNGMIMTNLVFGDLLILLSGLFYALDINMCKYVSDRIDAKKITQIASFFGGGF
                     ALFLILIFQIPFEISLSHIPNIAVIGIFGTGISTFFFLIALRLIGAVRTILLYSSTSV
                     FGMIFAGILLREEIAAPNIISIILVFMGIYWLRNRFAMDEKTISDLKGKFKHGKL"
     gene            complement(1726..2340)
                     /locus_tag="VLB45_01760"
     CDS             complement(1726..2340)
                     /locus_tag="VLB45_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HSD04463.1"
                     /translation="MIGVDTLLVKSLDTVIRANLSEKTLQKIENRLFEKYGMSITQSL
                     GEFQKLDEVLREFFGAGAEGLEKQILENVCKLEKTKDKDHEWLTIEDQALTKVILEAF
                     GDDDKKKILTSSIEDSHIISEILEKCAIPQTSGYRKINSMINDGLLITDGYINTADGK
                     KVNKYISVFRNVKIDIVKNKITIKVQIGKELLNHSQLIPIIQRS"
     gene            2430..2930
                     /locus_tag="VLB45_01765"
     CDS             2430..2930
                     /locus_tag="VLB45_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016940320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HSD04464.1"
                     /translation="MSRHVMDENNIRELPPRERLNHCARIIKNSDDESQRWDAVWLAG
                     EIAEMADPKDPIFDEVADLMCWVMENDNNGVVKHEAAFQVAARNMRKKIPDLVHAALH
                     DKSELMKHESLESLGLMRAFEAKDLITKALDDPSVDVQQTAAFVLKRFARLENLGTEY
                     KTYKIL"
     gene            3152..3928
                     /locus_tag="VLB45_01770"
     CDS             3152..3928
                     /locus_tag="VLB45_01770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HSD04465.1"
                     /translation="MSEPEKVPEQLQSSINFRVFIIILGLVIGFQFYINSIPDPEEAD
                     YIISVISILNPLAASITAFYVAKKYRDSKVFGRAYFALGLGLSMFALGEISYGALDLL
                     GAETYPSIADVFFFLFYPLGLYHLITNIRFFKPKLGITTKAIVVIIPVSIIAIYSYLS
                     FNAIGEANFDYYYGLIFIIASSLVLSAGILGARIFRQGILGVAWLVLVIGIVLTTTGD
                     VWYYYLETFDQYDLTHPVNLFWYASYMVITYALYKHQNLI"
BASE COUNT         1243 a          659 c          664 g         1365 t
ORIGIN      
        1 tatccgctct tgattatatt ttcaatactg tacatatacc cattaagaaa agcgatttca
       61 aaaaaacttg taatctcaac attaacaata tccatcgcat tattagttcc aactttctat
      121 accgtataca atgctaattc cgaagcaaat gaatttgaag ttgcattagc agcaagctat
      181 cctatacttg atgcaattgt tttatgtcct gcactgatag gagttacatt gtttttcaaa
      241 ggtgaagtaa attttctatg gtcgcttgtt tgtattggaa ttgtgttgaa tgttgtaggt
      301 gatacgggat tcttgatact gtctatcgac gaatcatact atacgggaca tcctattgat
      361 atgttgtatc tctgggcgta tactttgttc tcatttggag tctatagcca catcaagata
      421 ttcaaaagcc acaaaaaaga cacttacgaa aatattgatt cacttagatg ataacagtta
      481 gttggcaatg atctatgtca aagcttaccg tgtttgaatt tgcctttaag atcactaatg
      541 gttttttcat ccatggcaaa tctgtttcta agccaatata tccccataaa cactagaatt
      601 atggaaatga tgttgggtgc tgctatctct tcacgcaaaa gtattcctgc aaaaatcatt
      661 ccaaatacag atgttgatga ataaagaaga atagttctta ctgcaccaat taatctcaaa
      721 gcaattagga aaaagaacgt ggatatgcct gtcccaaaaa taccgatgac agctatattt
      781 ggtatgtgtg acaaactgat ttcaaatgga atctgaaaaa ttaaaattag aaataacgca
      841 aagcctcctc caaagaaaga tgctatctgt gtaatttttt ttgcgtcaat tctatcacta
      901 acatatttgc acatgttaat atctagggca taaaataatc ctgacaataa aattagtaaa
      961 tccccaaaaa ccaagtttgt cataatcatt ccattctggt aaagatcgta tcctaatggc
     1021 aatagtacca ctcctgcaat taccagggaa aatggcacag actctttttt ttgtaatttt
     1081 tccctaaaaa ttacaattgc tattaataat gaaaaaatta tttcaccatt agtaagaatt
     1141 gacgcattta ctgctgtgga ttcctttaat ccaaaaaagt atgtaataat ggctgagact
     1201 tcagctatgc ctataaataa taaaagcatg atattttttc gtccaatttt tgttactcta
     1261 tctccattct tttttactgg agtgaagaat aacccattga tgataaaaat tacagctgct
     1321 aaggtgagtg ggtttatctc aacaagatta acatcagaat gttctagcat tggctttgat
     1381 actgagtgaa taagacccgc taaggctgct gcacatatga ccaaaacata accaagtcga
     1441 ggagaagatg agtgtgtgag tttttctata aatgatattt tcggtaatgg caactatttt
     1501 ccattagaaa ttcctcaaga tcttaataac ctaggattgt atctgatttg actattggtt
     1561 aaaactttgc tactagtaat attactacgg ggtttttatc aggcaattga aggtggagaa
     1621 ctcgacatgc tgaaaactac tgtgcattga caaaaaattt gttatgtgac tttgacatat
     1681 tttgaaataa aaaactacca gtattgatat gtgttaaaac agaatttatg atctttgtat
     1741 tattgggatt agttggctgt ggtttaaaag ttccttgcct atctgaactt tgatagtaat
     1801 cttgtttttc acaatatcta ttttcacatt tctgaatact gaaatgtatt tgttaacctt
     1861 ttttccgtct gctgtattta tgtaaccatc agttatcaac aatccatcgt tgatcatgct
     1921 gttgattttt ctgtatcctg atgtctgtgg tatggcgcat ttttccaaga tctcagaaat
     1981 aatatgtgaa tcttctattg atgaagttaa aattttcttt ttatcatcgt caccaaatgc
     2041 ttcaagaatt acctttgtaa gtgcttggtc ttcgatagta agccattcgt gatctttgtc
     2101 ttttgttttt tctagtttgc aaacgttttc caaaatctgt ttttctaaac cttcagctcc
     2161 agctccgaag aactctctta atacttcatc tagtttctga aattctccaa gggattgggt
     2221 aattgacatt ccatattttt caaagagtcg attctctatc ttttgtaatg tcttttcgct
     2281 tagatttgct ctaatgaccg tatctagcga ttttaccagt agtgtatcta ctccaatcat
     2341 atggagttta tctcatgtac ggttaataag aaattgtctt tttgtaatat gacactaaat
     2401 taatataccc agcattccca aatatggaaa tgagccggca tgtaatggac gaaaataaca
     2461 tacgagagct gcctcctaga gaacgtctga atcactgtgc acgcataata aaaaattctg
     2521 acgatgaatc tcaacgttgg gatgctgttt ggcttgcagg cgagatagca gagatggctg
     2581 atcctaaaga tccgatattt gatgaggtag ctgatctgat gtgctgggta atggaaaatg
     2641 ataacaacgg agttgtaaaa catgaagccg cctttcaagt agcagccaga aatatgagaa
     2701 aaaaaattcc agatttggtg cacgcagcat tacacgacaa aagtgagtta atgaagcatg
     2761 aatcactaga atcattggga ttgatgcgag cgtttgaggc caaggatcta atcaccaaag
     2821 cattagatga tcctagtgtg gatgttcaac aaaccgcagc ctttgtgtta aagagatttg
     2881 ctagattgga gaatcttgga acagaataca aaacctataa aatcctctag tcaaataaat
     2941 tcttaaattt ttcttaattt ttctaatttt tgctcatttt ataaattaga tttaacaaaa
     3001 ttagtaatat gtaatatggt aatacatacg tcagaatata cttgatagat gaagattttt
     3061 tatatgttgg accagaaaaa ctgtaaattt cacaatgtct tgcctgccgt atcaattagt
     3121 ctttccaatt acacatttga agtgattaga aatgtctgag ccagaaaagg ttcctgaaca
     3181 actacaaagt tcaattaatt ttcgagtctt tataattatc ttaggattag ttattggttt
     3241 tcaattttat attaattcta ttcctgaccc agaagaagct gattacataa tctccgttat
     3301 ttctattttg aatccattgg cagcctcaat cacagcattt tatgtagcta aaaaatatcg
     3361 tgattcaaaa gtttttggac gcgcatattt tgcattgggt ttgggattat ccatgtttgc
     3421 attaggagaa attagttatg gagcattgga tctattaggt gcagaaacat atccttctat
     3481 agctgacgtt ttctttttcc ttttttatcc tctcggatta tatcatctta taacaaatat
     3541 cagatttttc aaaccaaaat taggtattac aacaaaggca attgttgtaa ttataccagt
     3601 ttcaataatt gcaatttatt cttacctttc atttaatgca ataggcgaag ccaactttga
     3661 ctattattac ggattgatat ttatcatagc ttcttcatta gtactctcag caggaattct
     3721 aggtgcaaga attttccgcc agggaattct tggcgttgcc tggcttgttc tagtaattgg
     3781 tatagtactt acaaccacag gtgatgtttg gtattattat ttagaaactt tcgaccaata
     3841 cgacttgaca caccctgtga atctattttg gtatgcaagc tatatggtta ttacttatgc
     3901 gctatacaaa catcagaatc tgatttagat t
//
LOCUS       DATIDL010000040         5500 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrosopumilaceae archaeon isolate SMAG_U16342
            k141_1868777, whole genome shotgun sequence.
ACCESSION   DATIDL010000040 DATIDL010000000
VERSION     DATIDL010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935836
            Sequence Read Archive: SRR25158326
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosopumilaceae archaeon (soil metagenome)
  ORGANISM  Nitrosopumilaceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrosopumilales;
            Nitrosopumilaceae.
REFERENCE   1  (bases 1 to 5500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/04/2024 05:09:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,520
            CDSs (total)                 :: 1,474
            Genes (coding)               :: 1,474
            CDSs (with protein)          :: 1,474
            Genes (RNA)                  :: 46
            rRNAs                        :: 1 (5S)
            complete rRNAs               :: 1 (5S)
            tRNAs                        :: 43
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5500
                     /organism="Nitrosopumilaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1868777"
                     /isolate="SMAG_U16342"
                     /isolation_source="soil"
                     /db_xref="taxon:2202732"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1130
                     /gene="ahcY"
                     /locus_tag="VLB45_01775"
     CDS             <1..1130
                     /gene="ahcY"
                     /locus_tag="VLB45_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007550766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HSD04466.1"
                     /translation="PLRGLRLGVCLHITKETSVLIMGAKELGADVIVCAANPLSTQDD
                     IAAFLASNGIHIYAWKGQTSKEYDWCIEKVLSHKPNILTDDGSDCHSKVHGNKKYSSL
                     KVIGGTEETTTGVIRLKALAEQKKLRYPVIAVNDAYTKHMFDNRYGTGQSTMDGYLRA
                     MNLLIASKRVVIAGYGWVGRGVAARAHGRGAKVYITEVDPVRALEAHMDGYEVLTMDE
                     AAKIGDIFITATGQTGVIRKEHILKMKEGAIMGNVGHFDVEVDANFLLSQSKSVKEIR
                     PNLDECLLKNGKRVYLIGKGRIANLVAAEGHPPEVMAQSFSNQLLSMIYISKNHKKIG
                     KKVITVPHEIDQQIAIDALGAMDVKIDKPTAAQLKYAHSWG"
     gene            1161..2810
                     /gene="metG"
                     /locus_tag="VLB45_01780"
     CDS             1161..2810
                     /gene="metG"
                     /locus_tag="VLB45_01780"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HSD04467.1"
                     /translation="MSNRAVITSALPYANGEIHLGHVASTYLPADITTRYLKQKGVEA
                     YYICASDDFGTPILIQSEKEKKTPEEYVKFWNQRDYEDFTALGIKFDFFYRTSSPENI
                     VFVQDVFKKLLKNDHIYEKEIVQFYCKFDKKFLPDRYVIGTCPYCKAEDQYSDLCEKC
                     GRVPEEIENPRCSLCKNPPTKEKTKHYFFKLTNFVKDLNEWLERDSHLQKDVKKYVQN
                     WIKEGLIEWDITRDITWGVPIPSPDAKDKVFYGWFDNHLAYISSALKFLNEKGIDGKA
                     FWNSADIYHFIGKDIVYHHYLFLPAMRIGIEKEYKLPDYIPTRGHLMLQAKKISKSRN
                     WYIGLKEFLSFYPADYLRFYLSSITPYSQDDLNFDWDEFATRINSELIGNLGNFVNRA
                     LGFTQKTLAGTIPDKEEFDSLDKEIEEKIKGLSSAVGELMEQNHLDRALKKILEFSAF
                     FNQYFQRKEPWKNGPGTNNCVYLSVNAVRSIAIALFPFLPESAEKIWTQLNLKGKVEE
                     QKWNSISEFGVAPKHVLGTVSPLFKRVEDSEIKIHKAKLGS"
     gene            2997..3995
                     /gene="ilvC"
                     /locus_tag="VLB45_01785"
     CDS             2997..3995
                     /gene="ilvC"
                     /locus_tag="VLB45_01785"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HSD04468.1"
                     /translation="MAKTWKDDQVSLDPIKNQTIAVIGYGIQGHAQSNNLKDSGLKVI
                     VGVKEGGESWKKAKADGHEVLPISKACEKADIIHILIPDMLQAKVFKEEIEPHLKAGK
                     ALSFSHGAAIYWKWITAPNNVDVIMVAPKGPGSKVRETYLDGFGTPSIVAVEQDYTKT
                     AWDRTLGIAKGIGSTRAGIIQTTFKEEVETDWFGEQADLCGGCASMVMNSFETLVEAG
                     YQPEIAYFEVLHELKLIVDMIQRYGINGMWRRVSETARYGGLTRGPMVMDKNVKENMK
                     KVLKMIQDGTFNNEWISDYQKNGKNAFDRYMKEIESHQIEQVGKKMRQMMWPDSKE"
     gene            complement(3997..4419)
                     /locus_tag="VLB45_01790"
     CDS             complement(3997..4419)
                     /locus_tag="VLB45_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008299486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HSD04469.1"
                     /translation="MSQKTATRRVKILVAKLGLDGHDRGALVLCRAFRDAGMEVIYSG
                     LFATPERIAKIAEDEDVDAVALSLLNGAHLTLFPRVVKALKEKGIKDVLVIGGGVIPE
                     DDKPELEKSGITGNFGPGTPLPTIIKHINSGVTKLRKI"
     gene            4573..5487
                     /gene="meaB"
                     /locus_tag="VLB45_01795"
     CDS             4573..5487
                     /gene="meaB"
                     /locus_tag="VLB45_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HSD04470.1"
                     /translation="MDIITGLKQGKRHAIARAISIIENEEKEARGLIKKIFKNSGKSI
                     IIGITGPAGAGKSSLINKTALELKKLGYKTAVLAIDPTSHLTGGAILGDRVRMSESTD
                     SGTYIRSMASRGATGAISRSVRNSIRVLEYAGFDPIIIESVGAGQTEIEISKISDITV
                     VVFNPNTGDSIQTIKAGLTEIGDIYLVNKSDLSGAGQLFNAVRDYIGMSEKNPQIMMT
                     STRTNKGISEFAKKLKELITKKKKTKLAEEKSRLETELRDIVLNNVKTKAERMLDSSA
                     TFTMYLKKVQSKTIDPFEAAEKISKSLK"
BASE COUNT         1941 a         1012 c         1143 g         1404 t
ORIGIN      
        1 aaccattacg cggattaaga ttaggagttt gcttgcatat cacaaaagaa acctcggttt
       61 taattatggg cgcaaaggaa cttggagctg acgtcatagt gtgtgccgca aatcctcttt
      121 ccacacaaga tgacattgca gcatttcttg catcaaatgg aatccacatc tatgcgtgga
      181 aaggacaaac atcaaaagaa tatgattggt gcattgaaaa ggtgctcagc cataaaccaa
      241 atattttaac tgatgatggc tctgactgtc acagcaaagt tcacggtaat aaaaaatatt
      301 cttcgctcaa agttataggt ggtactgaag aaactacaac aggtgttata cgactaaagg
      361 ctcttgccga gcaaaaaaaa ctgcgatatc ccgtaattgc agtaaatgat gcttacacaa
      421 aacacatgtt tgataacaga tacggaacag gccaaagcac aatggatgga tatcttaggg
      481 cgatgaactt gcttatcgct agcaaacgcg ttgtgattgc aggctatggt tgggtaggca
      541 gaggagttgc agcacgagcg catggcaggg gcgccaaagt atacatcacc gaagttgatc
      601 ctgttcgcgc actagaagct cacatggacg gttatgaagt tctgacaatg gacgaggctg
      661 caaaaatcgg tgacattttc atcactgcaa ctggtcaaac tggagtgata agaaaagaac
      721 acatcttgaa aatgaaagaa ggtgccataa tgggaaatgt tggccacttt gatgttgaag
      781 ttgacgcaaa ctttttgcta tctcaatcaa aatcagtaaa agagatacga ccaaaccttg
      841 atgaatgctt gctcaaaaat ggaaaacgtg tttatctaat tggcaaagga agaattgcaa
      901 atcttgtagc tgcagaagga catccgccag aagtaatggc gcagtctttt tcaaaccaac
      961 ttctttccat gatctacatt tcaaaaaacc ataaaaaaat aggaaaaaaa gtcatcactg
     1021 ttccacatga gatagaccaa caaattgcaa ttgatgcact tggcgcaatg gacgttaaaa
     1081 tagacaagcc aacggcagcc cagctaaaat atgcacacag ctggggatag cttaaattcg
     1141 tctcgcactt tacagaccta atgagcaata gggcagtaat tactagcgca ttaccatatg
     1201 caaacggcga gattcatctt ggtcatgtcg catcaacata tctgcctgcc gatatcacaa
     1261 cacgatatct aaaacaaaaa ggagtagaag catactatat ttgcgcatca gatgattttg
     1321 gaactccaat tctcatacaa tcagaaaagg aaaagaaaac tccagaagaa tatgtaaagt
     1381 tctggaatca gagagattat gaggatttta ctgcacttgg aataaagttt gacttttttt
     1441 acaggacaag ctcgcctgaa aacattgtat ttgtacaaga cgtgttcaaa aaattactca
     1501 aaaacgacca catctatgag aaagaaattg tccagtttta ctgtaaattt gacaaaaagt
     1561 ttcttccaga cagatacgtg atcggaacat gcccttattg caaggctgaa gatcaatact
     1621 ctgacctttg tgaaaaatgt ggacgtgtgc ctgaagagat agagaaccca aggtgttcat
     1681 tgtgcaaaaa tccgccaact aaagaaaaga caaaacatta ttttttcaag ctgacaaatt
     1741 ttgttaaaga cctaaatgaa tggctagaaa gagattcgca tttacaaaag gatgtaaaaa
     1801 aatatgtgca aaattggatc aaagaggggc tgatagagtg ggatatcaca cgagacatta
     1861 cttggggcgt gccaatccct tctcctgatg ccaaagacaa ggtcttttat ggatggtttg
     1921 acaatcatct cgcatacatc tcatctgcac tcaaatttct aaatgaaaaa ggaattgacg
     1981 gtaaagcatt ttggaattct gctgacattt atcatttcat tggaaaagac atcgtatatc
     2041 accactatct atttttgcct gccatgagaa ttggaataga aaaagagtac aagctcccag
     2101 attatatccc aactagagga catctgatgt tgcaggcaaa aaaaatatca aagagcagaa
     2161 actggtacat tggattaaaa gaatttctca gtttttatcc agcagattat ctcagatttt
     2221 atctttcatc aatcactcca tacagccaag atgatctgaa ttttgattgg gatgagtttg
     2281 ctacaaggat aaactcggaa ttaattggaa acctaggcaa ctttgttaat cgcgcactag
     2341 gatttacaca aaagacactg gctggaacaa ttccagacaa ggaggaattt gattcacttg
     2401 acaaggaaat cgaagaaaaa atcaaaggtc tctcatctgc agtaggagaa ttgatggaac
     2461 aaaaccatct tgatagggca ctcaaaaaaa tcttggagtt ttcagcattt ttcaaccagt
     2521 attttcagcg caaagagcca tggaagaatg gtcctgggac aaacaactgt gtctatctgt
     2581 cagtaaatgc agttcgcagc atagcaatag ctttgtttcc gtttttgcca gaatcagcag
     2641 aaaagatttg gactcagcta aacttgaaag gcaaagtcga agagcaaaaa tggaattcca
     2701 tttcagaatt tggtgttgca ccaaaacacg ttcttggcac agtctcacca ttattcaagc
     2761 gtgttgaaga ttctgaaatt aaaatccaca aagcaaaatt aggcagctaa tctttttgat
     2821 atggtaaggt tgtaaaattg aaaaaactgc gggcatgtcc catctgtcct aactgtggct
     2881 caaaaaaatt tgaaaagcta aaaactgagg agactcgggt caggtgctta aaatgcggaa
     2941 aagagattgt aatctaatct cgaaagatta tatttgcaat aatttgaccg ttttgcatgg
     3001 caaaaacatg gaaagatgat caagttagtt tagatccaat caagaatcaa acaattgcag
     3061 ttatcggtta tggaatacag ggtcatgccc agtcaaacaa cctcaaggat tctggcctga
     3121 aagttatcgt tggtgtaaaa gagggtggtg agagctggaa aaaggcaaaa gcagacggac
     3181 acgaggtact gccaatctca aaggcatgcg aaaaggctga cattatacat attttgatac
     3241 ctgacatgct tcaagccaaa gttttcaaag aagaaatcga acctcacctc aaggcaggaa
     3301 aggcattatc gttttctcat ggcgcggcaa tttactggaa atggattact gcaccaaata
     3361 acgttgatgt aataatggtt gcaccaaaag gtcctggctc caaagtcaga gaaacatacc
     3421 ttgatggttt tggcactcca tcaattgttg cagttgaaca agattataca aagacagcat
     3481 gggacagaac cctgggtatt gcaaaaggaa taggaagcac aagggctgga atcattcaaa
     3541 caacattcaa agaagaagta gaaactgatt ggtttggcga acaagcagat ctctgtggag
     3601 gctgtgcgtc aatggtaatg aattcatttg aaacactagt agaggcagga taccaaccag
     3661 agattgcata ctttgaggtt ctacacgagc tgaaacttat tgtggatatg atccaaagat
     3721 atggcatcaa cggcatgtgg aggcgagtca gtgaaactgc aagatatggt ggcttgacac
     3781 gcggacccat ggtgatggat aaaaatgtca aagaaaacat gaaaaaagtt ctaaagatga
     3841 ttcaggatgg aacatttaac aacgagtgga taagcgatta tcagaaaaat ggcaagaacg
     3901 catttgacag atacatgaaa gaaatcgaat ctcaccagat agaacaggtg ggaaagaaaa
     3961 tgcgtcagat gatgtggcct gactctaaag agtaaactag atttttctta gttttgtaac
     4021 gccagaattg atgtgtttga ttattgtagg taacggagtt ccaggaccaa agtttcctgt
     4081 aattcctgat ttttcaagct ctggcttgtc atcttctggg atgacgccac cgccaatgac
     4141 aagaacatcc ttgattcctt tttctttgag tgccttgaca actcttggaa acaatgtcag
     4201 atgtgcaccg ttaagaagac tcaacgcaac agcatcaaca tcctcgtctt ctgctatctt
     4261 tgctattctt tctggcgtgg caaataatcc agaatagatt acttccattc ctgcatctct
     4321 aaacgcccta cacaacacca gtgcgcctcg gtcatgaccg tcaagaccaa gttttgcaac
     4381 aagaattttc actctgcgcg tagcagtctt ttgtgacata atattaagcg gtctgttggg
     4441 ttttaaaatc tttatttgca caaaattaca aaatcaaatg aaaaagaatc ttaattattt
     4501 caggcaaagg tattcccagc agacctaaac aagaagattt tattgtggat attacccgta
     4561 attccttctg ggatggatat tatcactggg ttaaaacaag gtaaaagaca tgctattgca
     4621 agagccatat cgattattga aaatgaagaa aaagaagcac gaggattaat taaaaaaata
     4681 ttcaaaaatt ctggcaagtc aatcatcatt ggtataacag gtcccgcagg tgcaggaaaa
     4741 agctcactaa tcaacaagac tgcacttgag ctaaaaaaac tcggttacaa gactgcagta
     4801 ttggcaatag atcccacaag tcatctcact ggaggcgcta tacttggcga cagggtgcgc
     4861 atgtcagaat ctaccgactc tggaacatat atcagaagca tggcatcaag gggagctact
     4921 ggtgcaattt ctcgttctgt tagaaacagc atacgcgttt tagaatatgc aggatttgat
     4981 ccaatcataa tagaaagtgt tggtgcaggc caaacagaaa ttgagatctc aaaaatatca
     5041 gatattacag ttgtagtttt taatccaaac actggtgaca gcattcaaac aataaaggca
     5101 gggttaacag aaatagggga catttatctt gtaaacaaaa gcgatttgtc tggtgcagga
     5161 cagctcttca atgcagtacg cgattatatc ggcatgtcag aaaaaaaccc acagataatg
     5221 atgacttcga ctagaacaaa caagggcatt tcagaatttg caaaaaaact aaaagagctt
     5281 ataacaaaaa agaaaaaaac aaaactcgct gaagaaaaat cacgacttga aacagaattg
     5341 cgagacattg ttttaaataa tgtgaaaacc aaagctgagc ggatgctaga ctctagtgcc
     5401 acatttacaa tgtacctaaa aaaagtccag tcaaagacaa tagatccgtt tgaggcagca
     5461 gaaaagattt caaaatcatt aaagtgataa catgaaacaa
//
LOCUS       DATIDL010000041         7745 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrosopumilaceae archaeon isolate SMAG_U16342
            k141_658368, whole genome shotgun sequence.
ACCESSION   DATIDL010000041 DATIDL010000000
VERSION     DATIDL010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935836
            Sequence Read Archive: SRR25158326
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosopumilaceae archaeon (soil metagenome)
  ORGANISM  Nitrosopumilaceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrosopumilales;
            Nitrosopumilaceae.
REFERENCE   1  (bases 1 to 7745)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7745)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/04/2024 05:09:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,520
            CDSs (total)                 :: 1,474
            Genes (coding)               :: 1,474
            CDSs (with protein)          :: 1,474
            Genes (RNA)                  :: 46
            rRNAs                        :: 1 (5S)
            complete rRNAs               :: 1 (5S)
            tRNAs                        :: 43
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7745
                     /organism="Nitrosopumilaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_658368"
                     /isolate="SMAG_U16342"
                     /isolation_source="soil"
                     /db_xref="taxon:2202732"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1202
                     /locus_tag="VLB45_01800"
     CDS             <1..1202
                     /locus_tag="VLB45_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="adenine deaminase C-terminal domain-containing
                     protein"
                     /protein_id="HSD04471.1"
                     /translation="AKTMHTLKYMLEKGCVINGHTAGASDKKLNAYIASGILSCHEPI
                     DFAQVIERLRLGMSIMMREGSIRRDLDKMIPDILEHKPYLDRLMFCSDGVNPKDLIEL
                     GHIDHCVRKSVSLGLDKLDAITMATKNCFDYYNMLNDYGGIAPGKIADILVFDDLDKL
                     KPVKIFVGGKLVVSHGTIITKPKEPRIPSWITKTVKIKRKFQENDFVVKSTATSVQAL
                     VIKLHTEIITQKDQLELKVKDGNVVASADKDVWKVAAIDRIHGTNKHVVGFLQNFGAD
                     IGAFGSTSTFHENDMVIIGSSENDMAFVVNKLVEMRGGIVAVKDGKVLAAFALPYAGL
                     ISTLPFEKALVEYSKLNSTIVEAGCKFKNPYLIPLFLPFLALPDIRIIHTGIIDVKKR
                     TYLDILN"
     gene            1268..2896
                     /gene="thsB"
                     /locus_tag="VLB45_01805"
     CDS             1268..2896
                     /gene="thsB"
                     /locus_tag="VLB45_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thermosome subunit beta"
                     /protein_id="HSD04472.1"
                     /translation="MASIQQTPQGPVLVLKESALQQKGRDAQKNNIAAAKLVAELVRT
                     SLGPRGMDKMLVDSLGDVTITNDGATILKEIDVQHPAAKMMVEIAKTVDNEVGDGTTS
                     SVVFGGSLLAKAEELLDKDVHPTVIIEGFQAAAEKALSLLSELAKKVDPEEKEILLKI
                     ARTSMESKLVSEDSGMLSKLVVDAILQVVEKQGDSNKIDLDNIKVEKKAGGSIRNTSL
                     IKGIVIDKEVVHSGMPTKIEKAKIALVNSALEIEKTEMSAEIRITDPTQMQMFLEEEN
                     RMLKSMVDKIQEVGANVLICQKGIDDIAQHYLAKQGVLAVRRVKESDMTKLAKATGGR
                     ITTNLDDLTSKDLGSAEHVEQKKVESDKWVFIEGCRNPRAVTLLLRGGSQRVVDEVDR
                     SLHDSLMVVKDVIEKPAVVAGGGAPEEYLASALKDWADHFEGREQLAIKKYAEALEII
                     PLTIAENAGMDPIDTMITLRAKQSQGKKWTGIDARNTRIADMYSLDIIEPVAVKEQII
                     KSATEAACMILRIDDVIASSGSKGGGHAPHPPMG"
     gene            2906..3916
                     /locus_tag="VLB45_01810"
     CDS             2906..3916
                     /locus_tag="VLB45_01810"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HSD04473.1"
                     /translation="MLEQLQNLNIVGSVVVLNDFFLDRIIKIQNIMTLYDLISKKSQL
                     GGSIRGIPQTDLKGGNATNIAYALARLGAPISLITVADKTNTHILQDTFAEFSNAALF
                     TIDGTPGRTTSFEFSNNGRIVNIMLSDLGDNENFGPEKLGQKEQTAIQNADAIIVANW
                     ASNEKGTELSKFVFEKSTKSLHFLDPADIQSRPDEFKEAISELAPNLDSLSLNENECN
                     ILLKQHDLGSISGEDETKKLVSTLSEKISVVIDLHTSSGAFWSNGKETAFAKPFPTDV
                     KFVTGAGDVWDAANILGYLSGLEPKERLLFANATASLYVGNPKGIPPTMQEVLSLVTA
                     VQ"
     gene            complement(3900..5423)
                     /gene="guaA"
                     /locus_tag="VLB45_01815"
     CDS             complement(3900..5423)
                     /gene="guaA"
                     /locus_tag="VLB45_01815"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HSD04474.1"
                     /translation="MDKIVVLDFGSQYSHLICRRIREFSVYAELVPFDISLEELKKLN
                     PKGVIFAGGPASVYNSGSPKPQNGIFDLGLPVLGICYGHQLIVNSFGGKVKRANKEYG
                     SSSLTVDSNSDLFSGMGRSIRAWMSHGDAAEQIPIGFEIIGHTENSYAAAIANRQKLI
                     YGIQFHPEVVHTERGTEVLKNFVINICHAKQDWTIENFIESTVKEISKIDGNVLCGIS
                     GGIDSTVAALLIQKAIGNKLKCIFVDNGLLRLDEETEVEDMFKNNFSVNFTRVDAKER
                     FLSKLRGVKDPEQKRKIVGEEFVQIFTEFAEKNGPFKWLAQGTLYPDVIESGVSKGPA
                     AIIKTHHNVGGLPSWLNMQVLEPLRFLYKDEVRKVAKALGVPDKLSNRHPFPGPGLAV
                     RIIGEVTPKKLQICKLASKIVEDELITSGWYNKVWQAYAAVGDDKAVGVVGDERKYGH
                     IVIVRVVNSIDAMTADWTRLPAEVLERISNRITNEIDDAAWVSYVISSKPPATIEPQ"
     gene            complement(5428..5985)
                     /locus_tag="VLB45_01820"
     CDS             complement(5428..5985)
                     /locus_tag="VLB45_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04475.1"
                     /translation="MGYSEEEIRNILELKEWITEEIEKRKNEVEKLENNLSILDSVIK
                     QSSFSKASSLKSQNKPDTIIPEVGNSVIPIKTSDNMIIANAHVTPKEVVIVPSNNVTL
                     DAEMHPFKSFFLGRIIGGMESKDNLDVQSGKIDPDSVINCIINKDGNMIREILIRNYR
                     ERERVNEIINTVAWSFSRMIENSRK"
     gene            complement(6027..6767)
                     /locus_tag="VLB45_01825"
     CDS             complement(6027..6767)
                     /locus_tag="VLB45_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-hydroxymethylpterin diphosphokinase MptE-like
                     protein"
                     /protein_id="HSD04476.1"
                     /translation="MRLKGWDKRYSEILKEFNYSRKKDTESARILNYILKTKFPLKKL
                     EKKIKNQTIFVIGAGPSLVSSMHVLKKYKDITKIVADGATTALIQNKIRPDIVVTDLD
                     GQLEFLKKASTNNAVMVVHSHGDNIKKLQLASEFKFCIGTTEGKPFGKIFNFGGFTDG
                     DRCVFLANHFGANKIVLFGMDFGTKIGRYSKDVVQNRQNKLRKLRRGKKLLEWLAAKN
                     DTRFYTTSKPIKGFSKISYADLERIVPE"
     gene            6804..7670
                     /gene="folP"
                     /locus_tag="VLB45_01830"
     CDS             6804..7670
                     /gene="folP"
                     /locus_tag="VLB45_01830"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008300920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HSD04477.1"
                     /translation="MSKLGPVPVGKSNPVRVMGIMNLSPESFFKKSVITEKEKIASAT
                     RQMEEDGADFIDIGGMSTAPYLSTTVSKKTESERITRAIKIIQNISNIPISVDTCRSE
                     IAKDALELGVDIINDISGLKYDKYMPKIVEKYQPSLVLCAFSQNLVRGNLITQTKNLL
                     RQSIAIAKSAGISTNMVVDPAIGFFRKSGKGKFFTKIDSDWVQRDLLILNNLKSINLG
                     YPLLVSVSRKSFIGNLLKIKNPNDRLYGSLAAESIAVINGADIIRTHNVKETKNVIEI
                     AQKFSHPLRKGL"
BASE COUNT         2515 a         1456 c         1435 g         2339 t
ORIGIN      
        1 atgcaaaaac aatgcacact ctcaaataca tgctggaaaa aggctgtgtc ataaatggtc
       61 acactgcagg tgcgagtgac aaaaaactaa acgcctacat agcttctgga attctttcct
      121 gtcacgaacc cattgacttt gcccaagtca ttgaacgact taggcttggc atgtcaataa
      181 tgatgaggga aggctcgatt cgaagagact tggataaaat gatcccagac atactagaac
      241 acaaaccata ccttgacagg ctgatgtttt gttcagacgg agtaaaccca aaggatttga
      301 tcgaacttgg gcacattgat cattgtgtta gaaagtctgt gagccttggc ttggataagc
      361 ttgatgcaat tacaatggct acaaagaact gttttgatta ttacaacatg ttaaatgatt
      421 atggtggaat tgcaccaggt aaaattgcag acatacttgt ctttgatgat cttgacaaat
      481 taaaaccagt aaagatcttt gttggaggaa aacttgttgt atcacatggt acaataataa
      541 caaagccaaa agaacccaga attccttcat ggataacaaa aacagtaaaa atcaaacgaa
      601 aatttcaaga aaatgatttt gtagtcaaaa gtactgctac ttcagtccaa gctcttgtaa
      661 taaaactaca tacggaaata ataacgcaaa aagatcaact tgaattaaag gtaaaagatg
      721 gcaatgtagt tgcttctgct gacaaagatg tctggaaggt agctgcaatt gacagaatac
      781 atggtaccaa taaacatgta gtgggattct tacaaaactt tggcgcagat attggcgcat
      841 ttggctctac atcaacgttt catgaaaatg acatggtgat aattggttct agtgaaaatg
      901 acatggcctt tgtggtaaac aaactagttg agatgcgagg aggaattgta gcagtaaaag
      961 acggtaaggt tctagctgct tttgcccttc catatgctgg cttgatatct accttaccat
     1021 ttgaaaaagc actagttgaa tattcaaaat taaattcaac gatagtggaa gcaggatgca
     1081 aattcaaaaa tccttacctc ataccactct ttcttccgtt ccttgcgctc ccagacatac
     1141 gaattattca cactggaatt attgatgtca aaaaaagaac ctatctagat attctaaact
     1201 gacagaacct gaatcaataa ggtattaaaa ggggcattga actaactgaa aaccagaggt
     1261 aataattttg gcttcaattc aacaaactcc acaaggtcca gttctagtac taaaagaaag
     1321 tgcgctacag caaaagggta gagatgcaca gaaaaataat attgctgcag caaagcttgt
     1381 ggcagaactt gttcgcacaa gtcttggtcc aagaggaatg gacaagatgc ttgtggactc
     1441 tctaggcgat gttacaatta caaatgatgg tgcaaccatt ctcaaagaaa ttgatgtaca
     1501 acacccagct gcaaaaatga tggtcgaaat tgcaaaaaca gttgataatg aagtaggcga
     1561 tggaaccact tcctctgttg tatttggcgg ttcactttta gcaaaagcag aagaactctt
     1621 agacaaagac gtgcatccaa ctgtaataat tgaaggattt caggctgccg cagaaaaggc
     1681 actctctctt cttagtgaac ttgcaaagaa ggtggatcca gaagaaaaag aaatccttct
     1741 taaaattgca cgaacaagca tggaatcaaa actggtatct gaagatagcg gtatgctctc
     1801 aaaacttgta gttgatgcta tcttgcaagt agtggaaaaa caaggtgatt ccaacaaaat
     1861 tgacttggat aacataaaag tagaaaagaa agcgggaggc tcaatcagaa atacttcact
     1921 cataaaggga attgtcatag acaaagaagt tgtgcatagt ggaatgccga caaaaataga
     1981 aaaagcaaag atagcacttg taaattctgc attagaaatt gaaaagacag agatgagtgc
     2041 agaaattaga ataactgatc caactcaaat gcaaatgttt cttgaagaag aaaatcgaat
     2101 gctaaagtca atggttgaca aaattcaaga agtaggagca aatgttctga tttgccaaaa
     2161 aggtattgat gacatcgcac aacactatct tgctaaacaa ggagtacttg ctgtcagacg
     2221 cgttaaagaa agtgacatga caaagcttgc caaggcaact ggtggaagaa tcaccacaaa
     2281 cttggatgac ctgacatcaa aagatctcgg ttctgcagaa catgttgagc agaaaaaagt
     2341 ggaatcagac aagtgggtct tcatcgaagg atgcagaaat ccgcgtgcag taacacttct
     2401 cctaagggga ggttcacaaa gagttgtgga tgaagtcgat agatctctac atgattcatt
     2461 aatggttgta aaagatgtaa ttgaaaaacc ggcagttgtg gcaggcggtg gtgctccaga
     2521 agaatacctt gcatcggctc ttaaagactg ggcagatcac tttgagggaa gagaacagct
     2581 tgcaatcaaa aaatatgctg aagcactaga aatcattcct ctcacaattg cagagaatgc
     2641 tggaatggat ccaattgaca ccatgattac actgagagca aaacagtcac aaggcaaaaa
     2701 gtggactgga atagacgcaa gaaacacaag aattgcagat atgtattcac ttgacattat
     2761 agaaccggtt gcagtaaaag agcagataat caagtccgca acagaagcgg catgcatgat
     2821 tctaagaatt gatgatgtga ttgcctcatc cggttcaaag ggaggaggac atgcaccgca
     2881 tcctccgatg ggctaaaatc ttcgcatgtt agagcaactg cagaatctta acattgttgg
     2941 ttctgttgtt gtattaaacg acttttttct agacagaata ataaagatac aaaacatcat
     3001 gacactttat gatctgattt caaaaaaaag tcagcttggt ggaagcatac ggggaattcc
     3061 acagacagat ctgaaaggtg gtaatgcaac aaacattgca tatgcacttg caagacttgg
     3121 tgcaccaatt tcattaatca ctgtggcaga caaaacaaac acacacattt tacaagatac
     3181 atttgcagaa ttttcaaatg ctgccttgtt tacaatagat gggactcctg gaagaacaac
     3241 atcatttgaa ttcagtaaca acggacgcat tgtaaacata atgctctctg atcttggaga
     3301 caacgaaaac tttgggcctg aaaaattagg ccaaaaagaa caaactgcaa ttcagaacgc
     3361 tgatgcaatc atagttgcaa actgggcaag caacgaaaag ggaacagaac tatcaaaatt
     3421 tgtctttgaa aaatccacaa aatctcttca ctttctagat cctgcagaca tacaatctag
     3481 gcctgatgaa tttaaagaag cgatatcaga gctggcccca aatctggact ctttatctct
     3541 caatgagaat gagtgtaaca ttctgttaaa acaacatgat cttggaagca tttctggaga
     3601 agatgaaact aaaaaacttg tatcgacact ttcggaaaag atatctgttg taatagattt
     3661 gcacacatcc tctggtgctt tttggtcaaa tggaaaagaa actgcatttg caaagccttt
     3721 tccaactgat gtaaagtttg ttacaggagc aggcgatgtt tgggatgctg caaacatact
     3781 agggtatcta tctggtcttg aaccaaaaga aagattactt tttgcaaatg ctactgcatc
     3841 actttatgta ggaaatccaa aaggcattcc gccaacaatg caggaagtac tatcattggt
     3901 tactgcggtt caatagttgc tggtggttta cttgaaatca catagctgac ccaagctgca
     3961 tcgtctatct catttgtgat tctgttgctt attctctcta aaacttctgc tggtagtctt
     4021 gtccaatctg cagtcattgc atctatggaa tttacaacac gcacaataac aatatgaccg
     4081 tattttcttt catcacccac tactcctact gccttgtcat ctcctaccgc agcgtatgcc
     4141 tgccagactt tattgtacca accagatgtg atgagttcat cttctacaat cttacttgca
     4201 agtttacaaa tctgtaattt ttttggcgtc acttccccaa ttattctaac tgcgaggcca
     4261 ggtcctggaa atggatgacg atttgagagt ttatctggta ctccaagtgc tttagcaact
     4321 tttcttacct cgtctttgta aagaaaacgt aatggttcta gtacctgcat attcagccaa
     4381 gaaggcagac ctccaacatt gtggtgtgtt tttattatgg ccgcaggtcc ctttgagact
     4441 ccgctctcaa ttacatctgg gtagagtgtc ccttgtgcaa gccatttgaa aggaccattt
     4501 ttttcggcaa attctgtaaa tatctgcacg aattcctctc cgactatctt tcttttctgt
     4561 tccggatctt ttactcctct tagtttggaa agaaatcttt cttttgcatc aacacgcgta
     4621 aagtttaccg aaaaattgtt cttgaacatg tcttccacct cggtttcttc atctaatcgc
     4681 aaaagaccat tatccacaaa gatgcatttg agtttgtttc ctattgcctt ctggatcaga
     4741 agtgcagcta ctgtagagtc tattccccca cttattccac ataatacatt gccatcaatt
     4801 tttgaaatct cttttactgt actttctatg aaattttcta tagtccagtc ttgtttagca
     4861 tgacatatgt ttattacaaa attttttaga acttcggtac ctctttctgt atgtactact
     4921 tctggatgaa actgtatgcc atagattaat ttctgtctgt ttgctatagc tgctgcgtaa
     4981 gaattctcag tgtgacctat tatttcaaag ccaattggaa tttgttctgc cgcatcccca
     5041 tgactcatcc atgctcgtat agatcttccc atgccagaga acaaatctga attgctgtct
     5101 actgtaagtg atgaggaacc gtattctttg tttgcacgct ttacttttcc gccaaaacta
     5161 tttactatta attggtgacc ataacaaatt cctaaaacag gcaatcccag atcaaatatt
     5221 ccattttgtg gttttggtga gccactattg tatacactag caggaccgcc agcaaaaatg
     5281 acgcctttag gatttagttt ctttaattcc tcaagtgata tatcaaatgg tacaagttca
     5341 gcatatacgg aaaattctct aattcttcta caaatcagat gactatactg tgaaccaaag
     5401 tctaaaacaa ctatcttgtc cataaattca ctttctggaa ttctcaatca tgcgggaaaa
     5461 tgaccatgca actgtgttga ttatttcgtt tactctttct ctttcacgat agtttctgat
     5521 tagaatctct cttatcatgt tcccatcctt gttaattatg caatttatca cagaatctgg
     5581 gtctatcttt ccgctttgta catctaaatt gtctttactt tccattccgc ctattattcg
     5641 acctagaaaa aatgatttga acggatgcat ctctgcatct aatgttacat tatttgatgg
     5701 tacaataaca acttcttttg gtgtaacatg tgcatttgca ataatcatgt tgtcactggt
     5761 tttaattggt attacagaat ttccgacctc tggtatgatt gtatcaggtt tattttgaga
     5821 ttttagagac gatgccttgc taaaacttga ttgttttata acagaatcca gaattgataa
     5881 attgttctct agtttttcaa cctcgttttt gcgcttttct atctcttctg ttatccactc
     5941 ttttagttct aaaatattac gaatctcttc ttcagaataa cccatgtttt ttttaatagt
     6001 ttatggataa taaatattaa aacacgctac tctggaacaa ttctctcaag atcggcataa
     6061 gatatcttgc taaatccttt gattggtttt gacgtagtat agaatcttgt atcgttcttt
     6121 gccgcaagcc attccaggag cttcttacca cgtcgcaatt ttctaagttt gttctgtctg
     6181 ttttgtacta cgtcttttga atatcgtcct atcttagtac caaaatccat tccaaacaaa
     6241 acaattttgt ttgctccaaa atggttagca agaaatacgc atctgtcccc atcagtaaac
     6301 ccaccaaaat tgaaaatttt cccaaatggt ttaccttctg tcgtacctat gcaaaatttg
     6361 aattcagatg caagctgtaa ttttttaata ttgtctccat gggaatgtac caccattaca
     6421 gcattgtttg tacttgcttt tttgagaaat tccaactgcc catcaagatc ggttaccaca
     6481 atatctggtc ttatcttgtt ttgtattaga gctgtcgttg caccatctgc aacaatttta
     6541 gttatatctt tgtatttttt taatacgtgc atgctggaaa cgagggaagg accagcccca
     6601 ataacaaaga ttgtttgatt tttaattttc ttttctaatt tctttaatgg aaattttgtt
     6661 ttcaggatat aatttaaaat tctggcagac tcggtgtctt tttttctact ataattgaat
     6721 tcctttaaga tctcagagta tcttttatcc caacctttca atctcatgta agtctaaatg
     6781 tatgagggct aatcataaac catgtgagta aactaggccc tgttcctgta ggtaaatcca
     6841 atccagtgag agtgatgggg ataatgaatc tcagtcctga atcatttttt aaaaaatcag
     6901 taatcactga aaaggaaaaa attgcaagcg cgacaagaca gatggaagaa gatggtgctg
     6961 attttatcga tattggtggc atgtctactg caccttacct gagtacgaca gtttcaaaaa
     7021 aaacagaatc agaaagaata accagagcaa tcaaaataat ccaaaacata tcgaacattc
     7081 caatctctgt agatacttgt aggtcagaaa ttgcaaaaga tgcactggaa cttggtgtag
     7141 acataatcaa tgatatttca ggactaaaat atgacaagta tatgccaaaa attgtagaaa
     7201 aatatcaacc atctcttgtg ttatgcgcat ttagtcaaaa cttggttcgt ggaaatctga
     7261 taacgcaaac aaaaaatctc ttacgtcaaa gtattgccat tgctaaatct gctggtattt
     7321 caactaatat ggtagtggat ccggcaattg gtttttttag aaaaagcggt aagggcaagt
     7381 tttttacaaa aatagattct gactgggttc aaagagatct tttgatacta aataatctca
     7441 aatcaatcaa tctagggtat ccactgcttg tctctgtttc aagaaagtca ttcattggaa
     7501 atttattaaa aataaaaaat ccaaatgata gactttatgg ttcgcttgca gctgaatcta
     7561 tagcggttat caatggggca gatattatta gaactcataa tgtaaaagag acaaaaaatg
     7621 taattgagat tgcacaaaaa ttctctcatc ctctcaggaa aggcttataa cagattatta
     7681 ttctagacta gaaggtttag ttggaaatac gagaaggact cacatttgac gatgtgctcc
     7741 ttgtt
//
LOCUS       DATIDL010000042         4308 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrosopumilaceae archaeon isolate SMAG_U16342
            k141_859534, whole genome shotgun sequence.
ACCESSION   DATIDL010000042 DATIDL010000000
VERSION     DATIDL010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935836
            Sequence Read Archive: SRR25158326
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosopumilaceae archaeon (soil metagenome)
  ORGANISM  Nitrosopumilaceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrosopumilales;
            Nitrosopumilaceae.
REFERENCE   1  (bases 1 to 4308)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4308)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/04/2024 05:09:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,520
            CDSs (total)                 :: 1,474
            Genes (coding)               :: 1,474
            CDSs (with protein)          :: 1,474
            Genes (RNA)                  :: 46
            rRNAs                        :: 1 (5S)
            complete rRNAs               :: 1 (5S)
            tRNAs                        :: 43
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4308
                     /organism="Nitrosopumilaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_859534"
                     /isolate="SMAG_U16342"
                     /isolation_source="soil"
                     /db_xref="taxon:2202732"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..397)
                     /locus_tag="VLB45_01835"
     CDS             complement(<1..397)
                     /locus_tag="VLB45_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HSD04478.1"
                     /translation="MSENNFNIINFRVTFKNLPLHKLERFAFKDVTSACESFKKIPGV
                     SECVILQTSSRVEVFTANSLEEGEAPDVRRSEGKNLIIHKVVETWISLTGLEEYDVDH
                     FDQILEVYENTDVYHHLLRLACGLDSIVVG"
     gene            complement(410..1006)
                     /locus_tag="VLB45_01840"
     CDS             complement(410..1006)
                     /locus_tag="VLB45_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010194113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04479.1"
                     /translation="MPREKSSSSKNNPELTAAEVAELAEAAAKAAAAKAAADAQAQKE
                     YEEAAKVVAAVKAAAASFTDKRTQERIFRREMGEPEFFHYKKDLVPPRHVLTEEEQEE
                     ISRKVEIPSDQTPPYQPEHILSPEFGGTSDYESGIRDLLVETKRKLDRLRNDPHAMAD
                     VIAQTEQELTYLDSLYNNFYFGMNVFRTAKGGRSRLRE"
     gene            1168..1341
                     /locus_tag="VLB45_01845"
     CDS             1168..1341
                     /locus_tag="VLB45_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1059 domain-containing protein"
                     /protein_id="HSD04480.1"
                     /translation="MMKLICRDYGFDCNFIAQGEDIAKVIAEYSKHSIDEHGIEYSKE
                     ALMQFVLRKGMSP"
     gene            complement(1381..2226)
                     /locus_tag="VLB45_01850"
     CDS             complement(1381..2226)
                     /locus_tag="VLB45_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008301132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HSD04481.1"
                     /translation="MVAETKATITYVQLLKEDLVTLRITPNDGIIPDYEAGQFLTLGI
                     SVVAENYKIVRRAYSIASHPENKKYFEFILRWVRKPLPGRVTTQIFYASEGDQVSLGK
                     PTGNALTINQTLPDGSKDNRRIVCIGGGTGIAPFVSFAQHLRAVGDKREIVVLHGASY
                     VDELSYKSLFTRLEEESLDRGRDKWNFRYRGSISRPEEWFNRSWKGQTGRVENFLRSK
                     DGGMSPLEQLVGEKITKDNTMFYICGWQGTIDGVMDFMAPKGFVTFHNKREDGSFEVK
                     YESYG"
     gene            complement(2329..3111)
                     /locus_tag="VLB45_01855"
     CDS             complement(2329..3111)
                     /locus_tag="VLB45_01855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04482.1"
                     /translation="MSTNKPAKKSRSSSKATKADLEAKIAELEAKLNAVSSQLSKPAE
                     TKPAPAPQPKPETKPQHEARAPPPESTKPSEVAPAYSDTSSSQYTGNKYFATRQRLAY
                     HPPDKQFRGQVATTVQVSRPAPAPEPEPQQAPEPEPAPQEKRRRIEPPAPRVKYTGEN
                     YYKTRQRLAYHPSDKQFKEPTAIKFDEPPTAAQVESSTSSQSQSQSQSTSDKKSKTAQ
                     LAEYEREYLERVQQQKAESAKRAEAPSPAKPHRTMPAGWKPS"
     gene            3216..3596
                     /locus_tag="VLB45_01860"
     CDS             3216..3596
                     /locus_tag="VLB45_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04483.1"
                     /translation="MLDDFEKEYPGFDWKNTPAYKHPGGRNCPCPKHEYIREQINVAK
                     TVNQSNKEAGHLTLKFCPDHYKVYLNEVIPAMPFKYRMLTKIALRIGAIQIEQLKYMQ
                     SDQCFYCKFGSGGHDKKSELPPVS"
     gene            complement(3593..3823)
                     /locus_tag="VLB45_01865"
     CDS             complement(3593..3823)
                     /locus_tag="VLB45_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007550029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04484.1"
                     /translation="MSQQKCAVCSGQGKIALLETQCPFCNGTGEYTKSAESYLKSHIC
                     QCVFLDRVTCPLCGKKCHHDTPNKPKILLGPV"
     gene            3937..>4308
                     /locus_tag="VLB45_01870"
     CDS             3937..>4308
                     /locus_tag="VLB45_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010193277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthesis decarboxylase"
                     /protein_id="HSD04485.1"
                     /translation="MPIEDLNEFVEKLENAGELKRVKTQVDADLEIAEILRRTMYSNG
                     PALFFENVKNYTVPVLANAFGSLKRLEIGLETSDFTQIGQRIVDMTKMDIPSGLFDKI
                     RKLPELSKMTDIAPKLQKSCPV"
BASE COUNT         1217 a          828 c          812 g         1451 t
ORIGIN      
        1 ttcccacaac tattgaatcc aaaccacatg caagtcttaa taggtgatga taaacatcag
       61 tgttttcgta tacctcaaga atttggtcaa agtgatcaac atcgtattct tctaatcccg
      121 ttaacgaaat ccaagtttct acaactttat gtatgataag attttttcct tcacttcgtc
      181 ttacatctgg cgcttcacct tcctccagac tatttgctgt aaatacctca accctgctag
      241 aagtctgaag tattacacat tctgaaacgc caggaatttt cttaaaagat tcacatgctg
      301 atgtaacatc tttgaatgca aatctttcta atttatgaag cggaagattt ttgaaagtga
      361 ctctgaaatt aatgatgtta aaattatttt cgctcatttg gtatcactgt tactctcgaa
      421 gtctgctcct tccacctttt gccgtacgga aaacattcat gccaaagtaa aaattgttgt
      481 ataacgaatc caaatatgtt agctcttgtt cagtttgggc aataacatcg gccatcgcat
      541 gaggatcatt tctgagtcta tcgagttttc ttttggtctc tactaaaaga tctcgtattc
      601 cagattcata atctgacgta ccaccaaatt caggacttag tatgtgttct ggctgatatg
      661 gtggagtctg atctgatggt atctctactt ttcttgaaat ttcttcttgt tcttcttctg
      721 tgagtacgtg tcttggtgga actagatcct ttttataatg aaaaaattct ggctccccca
      781 tttctcgtct gaatattcgc tcttgagtac gtttatcagt aaatgatgca gctgccgctt
      841 ttactgctgc tactactttt gctgcttcct catattcttt ctgcgcttgt gcatctgctg
      901 cggcctttgc tgctgcggcc tttgctgctg cctctgccaa ttctgccact tctgcagcgg
      961 tcaattctgg attattttta gaacttgagg atttttcacg aggcattgta attacatcat
     1021 ttggggttaa attttaagtt tttaccattg gtatttttag tctaaatcag aacctaagat
     1081 gatacaattg taggtaaact cttgtccatg tctgatcgtt cataaaacta atgaaacgag
     1141 atatatacaa ccaatttttt gttataaata atgaagctaa tctgcagaga ttatggtttt
     1201 gattgcaatt ttattgcaca aggagaggac attgctaaag taattgcaga atacagcaag
     1261 cattctattg atgagcatgg aattgaatat tccaaagaag ccttaatgca atttgtgcta
     1321 agaaaaggca tgtccccgta atagacaaaa aacactgttc ttttaaaaaa tttttaatga
     1381 ttaaccgtat gattcgtatt ttacttcgaa gcttccatct tcacgcttat tatgaaaagt
     1441 tacaaatcct tttggtgcca taaagtccat aacaccatct atagtacctt gccaaccaca
     1501 aatgtagaac atcgtattat cttttgtaat tttttctcca acaagttgtt ctaaaggcga
     1561 cattccacca tctttagatc ttaagaaatt ttcaactctg cctgtttggc ctttccatga
     1621 tctgttaaac cattcttccg gtctgcttat agaaccacga tatctaaaat tccacttgtc
     1681 tcttcctcta tccaggcttt cttcttccaa cctagtaaag agacttttgt aacttaattc
     1741 atctacataa ctagcaccgt gtaaaacaac aatctctctt ttatcgccta ctgcacgaag
     1801 atgttgtgca aaacttacaa agggcgcaat acccgtaccg cctccaatac agacaattct
     1861 tcgattatct ttactaccat caggcaatgt ttgattaatt gttaaagcgt ttccagtggg
     1921 ttttcccaaa gatacctgat caccttcact tgcgtagaat atctgggttg taacacgacc
     1981 aggtaagggc ttgcgaaccc atctgagtat aaattcaaaa tattttttgt tttcagggtg
     2041 cgaagcaatc gaatatgctc tgcgaacaat cttgtaattt tcagctacta ctgaaatacc
     2101 gagggtaagg aattgacctg cctcataatc tggtataata ccatcatttg gagtgatacg
     2161 taaagtcaca agatcttctt tgagtaattg aacataagta atagttgctt tagtctctgc
     2221 gaccattctt aattgatctg atattcaata gttaaatatc ttatgaaaaa atagcgcaat
     2281 catacacata cgctaattct acacttgctt tttaaaaatt aaaatagttt aagacggttt
     2341 ccagccagct ggcatggttc tgtgtggttt tgccggagat ggtgcttctg cgcgttttgc
     2401 agattcagct ttttgttgtt gcactctctc tagatattct ctttcgtatt ctgctagctg
     2461 tgcagtttta gattttttat cagatgttga ttgtgactga gattgcgatt gtgagcttgt
     2521 agaggattca acttgggcag cagtaggtgg ttcatcgaat tttattgcag ttggctcctt
     2581 gaactgttta tcagacgggt gatatgccag tctttgtctg gttttatagt aattttcacc
     2641 agtatatttg actcttgggg caggtggttc aattcttctt cgtttctctt gtggtgcagg
     2701 ttctggttct ggtgcttgtt gcggttcagg ctctggagca ggagctggtc gagacacctg
     2761 aactgtagta gcaacttgtc ccctgaattg tttgtcaggt ggatgatatg ccaatctttg
     2821 ccttgttgca aagtatttgt taccagtata ttgtgatgag cttgtatcag aatatgctgg
     2881 tgctacttca gatggttttg tagattctgg tggaggtgct ctggcctcat gctgtggttt
     2941 agtttcaggt tttggctgtg gtgcaggagc tggctttgtc tccgcaggtt ttgacagctg
     3001 tgaagaaact gcatttagtt ttgcttcaag ctcagcaatc tttgcttcaa gatcagcttt
     3061 tgtggcttta gaagatgaac gtgatttttt agcgggttta ttggtagaca tgtgctattt
     3121 tgtgagtatg ttggagttta tttatatttt gatcatgggg caagtatatg aaggagcctc
     3181 atagaagttt tataagaacc acgaaccaac ttgatatgtt ggatgatttt gaaaaagagt
     3241 atcccggctt tgactggaaa aatactcctg catataagca ccctggtggc agaaactgtc
     3301 cttgccccaa gcacgagtac atcagagaac agatcaatgt agcaaagaca gtaaaccagt
     3361 caaacaagga agcaggtcat cttaccttga aattctgccc agatcattac aaggtttatc
     3421 taaatgaagt aattcctgca atgcctttca aatacagaat gcttacaaag atagctctaa
     3481 gaattggtgc catacaaatt gaacagctaa aatacatgca atcagaccag tgcttttact
     3541 gcaaatttgg atctggcggt catgacaaaa aaagcgagct tcctccagta agctagacag
     3601 gcccaagcaa gatcttgggt ttgtttggcg tatcatgatg gcattttttc ccacataatg
     3661 ggcatgtcac cctgtccaag aaaacacatt gacatatgtg agattttaga taactttcag
     3721 cactttttgt atattcacca gtcccattac aaaatggaca ctgtgtttct aaaagagcaa
     3781 tctttccttg cccgctacat acagcacatt tttgctgact cactataaag aattccctga
     3841 gtgtaattaa aatccttttg ttaaaacttt ttcctacttg cctatccttg gacaaatcca
     3901 gttaagcttt atactagata caaattcagt aaagttatgc ccattgagga tctaaatgag
     3961 tttgtagaaa agctagaaaa cgcaggagag ttaaaacgcg ttaaaacaca agttgatgcc
     4021 gatttagaaa ttgcagaaat tttaagaaga acgatgtatt caaacggtcc tgcattattt
     4081 tttgaaaatg taaaaaatta caccgtgcca gttttggcaa atgcttttgg ttctctgaaa
     4141 agacttgaga tcggtcttga aacttcagac tttacacaaa taggtcagag aatagtcgat
     4201 atgacaaaga tggatatccc atcaggactt tttgataaaa ttagaaaact tccagagctt
     4261 tcaaagatga cagatattgc accaaaatta caaaaaagct gtccagtg
//
LOCUS       DATIDL010000043        10447 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrosopumilaceae archaeon isolate SMAG_U16342
            k141_2474950, whole genome shotgun sequence.
ACCESSION   DATIDL010000043 DATIDL010000000
VERSION     DATIDL010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935836
            Sequence Read Archive: SRR25158326
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosopumilaceae archaeon (soil metagenome)
  ORGANISM  Nitrosopumilaceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrosopumilales;
            Nitrosopumilaceae.
REFERENCE   1  (bases 1 to 10447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/04/2024 05:09:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,520
            CDSs (total)                 :: 1,474
            Genes (coding)               :: 1,474
            CDSs (with protein)          :: 1,474
            Genes (RNA)                  :: 46
            rRNAs                        :: 1 (5S)
            complete rRNAs               :: 1 (5S)
            tRNAs                        :: 43
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10447
                     /organism="Nitrosopumilaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2474950"
                     /isolate="SMAG_U16342"
                     /isolation_source="soil"
                     /db_xref="taxon:2202732"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..396
                     /locus_tag="VLB45_01875"
     CDS             <1..396
                     /locus_tag="VLB45_01875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04486.1"
                     /translation="NDPEKQKLVEYCTSTELKDENGNFLGNIHMIGTIDAPRLVFVVV
                     QSNPVDTGQVKTVFDVSTKDLVCDCWDQVKPGEYETIERWIDALRDFHTGGDKPHSSS
                     KPLLLASKHLQIEVTTVKDGYVWEMLIAK"
     gene            477..1235
                     /locus_tag="VLB45_01880"
     CDS             477..1235
                     /locus_tag="VLB45_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016939268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HSD04487.1"
                     /translation="MFVDLWLILLGFVAGILGSMIGLGGGIVIVPILTFLGFAPAVAA
                     SNSLFAAFSNAIASTISYSKQKRIEYSLGLKFGLLSIPGTILGALISDEITSSLFKIL
                     FGIVLLASSVYIFSKRKMESKTYNLSKQIMVLAAGASFFAGIISSLFGIGGGLVFVPL
                     MVVIIGLSMKIAAPTSQFILLFASASGMIAHTFLGHPDFYQALLLAAGAFGGGLVGSR
                     LSLEVGEKKLKILVSAVMVVAAIKLFIDSFENPF"
     gene            complement(1207..1665)
                     /locus_tag="VLB45_01885"
     CDS             complement(1207..1665)
                     /locus_tag="VLB45_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04488.1"
                     /translation="MKKFSELKVGDEFFSVGKISLEELESYLKFSGIKNVMYEKDSES
                     TKKMVSGRAILGRMEGEFTRLEEMYGNSMIFYGMDGDPSWKNRHARFLKPLYTDENLK
                     MKFSISEKIDLNENYGLVAIDFEGTKENGDLVVVSRRNLYQIKKDFQTNQ"
     gene            complement(1665..3452)
                     /locus_tag="VLB45_01890"
     CDS             complement(1665..3452)
                     /locus_tag="VLB45_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HSD04489.1"
                     /translation="MLKFQGKVAVITGSGTGIGKAIATKFVENGASVVILGRRKEPLE
                     EAAKGLQEIINKVKSNAFVKIFAGVDVSDDAGISSMFDELKKSNITVDVVVNNAGVSG
                     PVTCFANSPIEEFKSTVAIHLTGTFWTSVQALKAMKPGSKIITISTFFAEERPLEQRP
                     YRFRSPYTASQGAKNRLAEAMSWELTEKGIISIATNPGPVHSDRIYKTVYPKAAAEFM
                     RVSGFESLTPQDVEQVNVDILPLMGEEEKTVKDGIKNAAAKLAKTKGITSDSEIQKLE
                     STTASLLSKIQQIAEKIQLNTSKMIADEQFLSQPQVAETVLALCDDAIAKILNGKVIP
                     GDRVFYPVKPHITSSIPETNTDFGSKVVVFTVDATEDSDIARVEFLAQNIEKSGGKVV
                     LLFSKNTPKQSQERLSKFHSHVIDLANQQEVRRMLNTASQKIGAISMFIHITGKVPSI
                     SKITEASRSQWDSLVDKFINTPATVGQECLGAFVPGGAKNPPLFKGKSGTMVIIGPDL
                     PSGPKVTGAERARVDVFRGALRPFSTTVNQELSDVLKSNLRIFLVLAGSIEGTEPNNE
                     RIWSAIKYFASGKATSSSEVIFYPDETRS"
     gene            3553..3789
                     /locus_tag="VLB45_01895"
     CDS             3553..3789
                     /locus_tag="VLB45_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04490.1"
                     /translation="MHDTESDTITLKTKPAEFCHLLEEIGIDGQPKEIATDEVEKGDY
                     YSRYFAQTPRMVTNKGCIELKGTNIDVVQIIQKG"
     gene            3792..4169
                     /locus_tag="VLB45_01900"
     CDS             3792..4169
                     /locus_tag="VLB45_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007401858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04491.1"
                     /translation="MQDPNPLPWGAQDRFQAHFIVKGNVGSKDLDFVAETKLKTKGHF
                     GSKTLVGVEWVGGKLAEALNGDSELASMILKQPINDAAIWVDPTKNGVRIYGKWKADH
                     ELGITKDLFAVYDRIASHIKKEL"
     gene            complement(4166..4702)
                     /gene="endA"
                     /locus_tag="VLB45_01905"
     CDS             complement(4166..4702)
                     /gene="endA"
                     /locus_tag="VLB45_01905"
                     /EC_number="4.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000214 - tRNA-intron endonuclease
                     complex [Evidence IEA];
                     GO_function: GO:0000213 - tRNA-intron endonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006388 - tRNA splicing, via
                     endonucleolytic cleavage and ligation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-intron lyase"
                     /protein_id="HSD04492.1"
                     /translation="MKGELIENRIVIWNVQDSRSLFMQGYYGKPIGITKPKPDEITVP
                     LILDLIEGYFLLQKSKIKVYRAKKQISENDLLAICRQEYHNFDKKYQVYKDFRDKGYI
                     VNPGIKFGCDFAVYQKGPGIDHAPYLVQVYNTSDTITATGVVLAGRLATTVRKQFILA
                     IPRGKDKVDCLALDWWRA"
     gene            complement(4777..5040)
                     /locus_tag="VLB45_01910"
     CDS             complement(4777..5040)
                     /locus_tag="VLB45_01910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04493.1"
                     /translation="MKKIKDKTFLLIAIGVGGAVIIGDLGMHFLFGPDDTPINNAVSI
                     IDKIESKNMDESAARLAIGNDPSESDIMRPEGWEGKFANFTKP"
     gene            complement(5043..5696)
                     /locus_tag="VLB45_01915"
     CDS             complement(5043..5696)
                     /locus_tag="VLB45_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HSD04494.1"
                     /translation="MYSGELEVQAKRKALAVLQDEINRILNAVRDLSALPALIVKGDK
                     SEIKASLERMRNTEDEVEALRRKITREIADVGGLMMNRENLLHTAYTMDEIAGYITGI
                     AFKLSNIKAMTLKKAKLDKDLTDLINLVVDEIYKLNEIIRALSGNPAGAIELAHEAQK
                     IERNIDDKYRELTIVVLDEITSIKELLLLKDVVGGVEEMADKCLEVSDSFIALALSL"
     gene            complement(5782..7554)
                     /locus_tag="VLB45_01920"
     CDS             complement(5782..7554)
                     /locus_tag="VLB45_01920"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HSD04495.1"
                     /translation="MQFSLVAETLKIMESTTKRLELTQYLVDLFKKTPPETISSVVYL
                     LQGKLRPDHEGVELGVAEKIAIKAISKSAGIPQKKIETAYKETGDLGQAAAKILEQKT
                     QTTFMTEDITVERVYDTLYKIAELKGSRSQDMKLKYISSLLNDASPIESGFILKIITN
                     SLRLGIADFTIMDSLAVAFTGTKENRAVLEQAYNVSSDLGKVAAAVAKDGINGVKSFQ
                     VSVFSPIRPMLAERVKSPQEAHEKMGQEFAAEYKLDGERVQLHLQDGKVVLYSRSLEN
                     ITSYYPDIVEKVTKSLNTHETILEAEAVAINEDSGEFLAFQELMHRRRKYNIQKAVSQ
                     YPITINFFDVMFADGKSCLDLPYRERRKILEKIVKEDNFVKLMPMTIVKTDNEVEDAL
                     ENAIIVGCEGVMLKHLDSPYRAGARGSNWLKLKREYRNELGDSLDLVVIGAFFGRGRR
                     TGRYGALLLATYDEQTDTFPSICKVGTGFTDENLDQFYQILSDKITLKKNPRINSGLE
                     AEVWFEPEVVIEIVASEITLSPLHKTAMDAIRKGSGLALRFPKFTGKIRFEKTPENAT
                     TDQEIITLYNGQKRVVQEEKQNYI"
     gene            complement(7592..7885)
                     /locus_tag="VLB45_01925"
     CDS             complement(7592..7885)
                     /locus_tag="VLB45_01925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04496.1"
                     /translation="MGLDVIEEKNLNDIITYAVDYPRVVLSEVKSLVPQGANLGDFAY
                     GLYVGYVSGIFFDGFLARNKRFLNDDELSDFNAMFLKRSQEIWLKIKAHLQIK"
     gene            7965..8279
                     /locus_tag="VLB45_01930"
     CDS             7965..8279
                     /locus_tag="VLB45_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HSD04497.1"
                     /translation="MGGAKKQSPAQAEKSQQAEAAKKEGGSKKGEKKEQKSKSSISVI
                     INENQALNFIKNSKVFTVQEIARQEGVKISAANTFLQKLLKSGSVKRIGGYSGHHIYT
                     HA"
     gene            complement(8270..8650)
                     /locus_tag="VLB45_01935"
     CDS             complement(8270..8650)
                     /locus_tag="VLB45_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008298277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclophilin-like fold protein"
                     /protein_id="HSD04498.1"
                     /translation="MSAGSVSKIEVILEIKGKSQLKCELKRHLAPKTVGTMIRSLPIE
                     GNAHMMGNSFAYVETKINVGGERQRNQFKKGEIAFMPLGGSVCFFLNDSESTKALTPI
                     GNITSNIDALKELKSGDVFVITQA"
     gene            8704..9216
                     /locus_tag="VLB45_01940"
     CDS             8704..9216
                     /locus_tag="VLB45_01940"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007401747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit K"
                     /protein_id="HSD04499.1"
                     /translation="MSDEPEEIIPDTEEVAVEPEEPEVPVVEEVVETTEAVGEEEELP
                     EKKKKAKKSDLTPEELAEKERLQKIIDAREIIDVDPIHVPIEFETTGRGKIMIGPPTL
                     TRFEKARIMGARALQLSLGAPPFIQVPKEVATSLDLAYAELEKRVIPITIRRVFPNGD
                     FQNIPINQFD"
     gene            9268..9603
                     /locus_tag="VLB45_01945"
     CDS             9268..9603
                     /locus_tag="VLB45_01945"
                     /inference="COORDINATES: protein motif:HMM:NF014029.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HSD04500.1"
                     /translation="MSLLSEIDVKTHLVNNPSLPNTGKVVVIQRQPIMECALDILPII
                     SGPGRVTLSAKGDDIPTAVGVANVITENMMKGNAEIVDIIVDSENGPGQYGMTLISTI
                     QIIIARKLN"
     gene            complement(9600..10016)
                     /locus_tag="VLB45_01950"
     CDS             complement(9600..10016)
                     /locus_tag="VLB45_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HSD04501.1"
                     /translation="MLLPAEIETKTLIPALRAILAKKLVEQHKIQEEDISKMLGVTQA
                     AISNYIRGTRGDPELITKLLAIKEVSEMIDEIASNLSSNMAYTPASLSKFIGLCNYIK
                     SSLLICDIHHSLETNIDEAICKECENMLLKGPGSSF"
     gene            10136..>10447
                     /locus_tag="VLB45_01955"
     CDS             10136..>10447
                     /locus_tag="VLB45_01955"
                     /inference="COORDINATES: protein motif:HMM:NF012936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HSD04502.1"
                     /translation="MQEIFSNLQKQISEAVSSCKSNCISLSGGLDSSILACYVDKKIK
                     AIAVVTKDFPATDLMFSQLVAKQSGLDLIIKNATIEEILSAIEETIKILKVFNHIEIR
                     NS"
BASE COUNT         2925 a         2103 c         1981 g         3438 t
ORIGIN      
        1 aatgatccag aaaaacaaaa gcttgttgaa tattgcacgt caacggaact aaaagatgaa
       61 aatggaaatt ttcttggaaa tatccacatg atcggaacaa ttgatgcccc acgattggtc
      121 tttgttgtag tgcagtcaaa cccagttgat accggccaag taaagactgt atttgatgta
      181 tccacaaagg accttgtttg tgattgctgg gatcaagtaa agccaggtga atatgagaca
      241 attgaaagat ggattgatgc cttgcgagat tttcatactg gtggggacaa acctcatagc
      301 tcatcaaaac cgcttttgct agcctcaaaa cacctacaaa ttgaggttac tactgtaaaa
      361 gatgggtatg tctgggagat gctcatagct aaatgacaaa aaatcttacc agattaacag
      421 gttcattaag gttgacgtaa caattaatcc gtaactgtat cgtttggtgt cttacattgt
      481 ttgttgattt atggttgata ttgctaggat tcgtagcagg aattcttggc tctatgatag
      541 ggcttggtgg tggaattgtt attgtgccaa ttcttacgtt tttaggattt gctcctgcag
      601 ttgctgcaag caacagtctc tttgcagcct ttagcaacgc cattgcatcg acaatatcgt
      661 attcgaaaca aaaacgcata gaatattcgc ttggtttgaa atttggactg ctttcaattc
      721 caggaacgat acttggcgca ttgatctcag atgaaataac atcttctttg tttaagatct
      781 tgtttggaat tgtattgctg gcatcaagtg tttacatctt ttcaaaaaga aaaatggaat
      841 ccaaaacata caatctctca aaacaaatca tggttttggc agctggtgct agtttttttg
      901 ctggaataat atccagtttg tttggaatag gtggaggatt ggtgtttgtt cctctgatgg
      961 ttgttataat tggcctttca atgaaaattg ctgctccaac atcacaattc attttgttgt
     1021 ttgcttccgc gtctggtatg atagcgcata cttttctagg ccatcctgat ttctatcagg
     1081 cacttttgct tgctgcaggc gcatttggcg gtggccttgt aggaagtagg ctctcacttg
     1141 aggtaggtga gaaaaaacta aaaattcttg tatctgcggt gatggttgtt gccgcaatca
     1201 aactcttcat tgattcgttt gaaaatcctt tttgatctga tacaagttgc gtcttgatac
     1261 aacgaccaga tccccgtttt cttttgtacc ctcaaaatct atggctacaa gcccataatt
     1321 ttcattaaga tcaattttct ccgatatgct aaacttcatt ttcagatttt cgtcagtata
     1381 tagtggtttt aggaatcttg catgcctgtt cttccaagat ggatctccgt ccatcccata
     1441 aaaaatcata gaattgccat acatttcttc cagtcttgtg aattctcctt ccatccttcc
     1501 caagatggcc ctgcctgaaa ccatcttttt ggttgactct gaatcttttt cgtacatgac
     1561 gttttttatt ccagaaaatt taagatagct ttcaagctct tctaaagaaa tttttcctac
     1621 tgaaaaaaat tcgtcaccta ctttgagctc tgaaaatttc ttcattatga tcgtgtctcg
     1681 tcaggataga aaattacttc agagctagaa gtggcttttc ctgacgcgaa atattttatt
     1741 gcactccaaa tcctttcatt gtttggttcg gttccctcta tacttcctgc aagtacaaga
     1801 aatattctca gatttgattt tagaacatca cttaattctt gattgactgt ggtggaaaac
     1861 ggtcttagcg ctcctctaaa cacatctact cttgcgcgct ctgctcctgt tactttggga
     1921 cctgaaggaa gatctggacc tattatgacc attgttcctg acttgccctt gaaaagaggc
     1981 ggattctttg ctccgcctgg cacaaaggcg ccaagacatt cctgtccgac tgttgcagga
     2041 gtgttgatga acttgtcaac aagagagtcc cactgtgatc ttgaagcttc ggttatcttt
     2101 gaaatggaag gaaccttgcc tgttatgtgt atgaacatgg aaatggcgcc aattttttgg
     2161 gatgccgtgt ttagcattct tcttacctct tgctgatttg ccaaatctat tacatgcgaa
     2221 tggaattttg ataatctttc ttggctctgt tttggggtgt ttttggaaaa caaaagcacc
     2281 accttgccgc cagatttttc tatgttttga gcaagaaatt ctacccgtgc aatatcagaa
     2341 tcctcagtgg cgtcaacagt aaagaccacg accttggagc caaaatctgt gttggtctcc
     2401 ggtattgatg atgttatgtg cggctttaca ggataaaata cacggtctcc tggaattact
     2461 ttgccattta gaattttggc aatcgcatcg tcgcataatg ccaagacagt ttcggctact
     2521 tgaggctgtg acaaaaactg ctcatctgct atcatcttac ttgtgttgag ttgtattttt
     2581 tctgcaatct gctgaatctt tgaaagaagt gatgctgttg tactttctag tttttgtatt
     2641 tctgaatctg aagtgattcc ttttgttttt gccagctttg cagctgcgtt tttaattcca
     2701 tcctttactg ttttttcttc ctcgcccatt agtggcagta tgtcaacatt gacttgttcc
     2761 acatcctgag gtgtaaggct ctcaaagcca ctgactcgca taaattctgc tgctgctttt
     2821 ggatatactg tcttgtagat tctatccgag tggactggtc ctggatttgt tgctattgaa
     2881 attattcctt tttctgtaag ctcccaagac attgcttctg caagtctgtt ttttgctcct
     2941 tgtgaagctg tgtatggact gcggaatctg tatggtcttt gctctagcgg tctttcttca
     3001 gcaaagaatg tagatattgt tataatcttg gaacctggct tcattgcctt gagggcttga
     3061 accgaagtcc aaaaagttcc tgtaagatga attgctacag tgctcttaaa ttcttcaatt
     3121 ggagagtttg caaaacaagt tactggtcct gaaactcctg cattattgac cacgacatca
     3181 acagttatgt ttgatttttt caactcatca aacattgatg atattcctgc atcatcgctt
     3241 acatctactc cagcaaagat tttcacaaac gcattgcttt ttactttgtt aattatttcc
     3301 tgtaatcctt ttgctgcttc ttcaagaggt tcttttcttc tacccaaaat aacgacactt
     3361 gcgccgtttt caacaaattt agtagctatg gccttaccga ttccagttcc gctgccagta
     3421 attacggcaa ccttgccttg aaactttagc attgacagaa aattgctata tttttcgatt
     3481 atttattccg attgttctgt tttggagatt ttaagactaa tatttatttg agggagaaat
     3541 ttaccatccc gcatgcatga tactgaatct gataccatta cactaaaaac taaacccgct
     3601 gagttctgtc accttttaga agaaattggc atagacggac agccaaagga gattgcaacc
     3661 gatgaagttg aaaaaggcga ttattacagt agatattttg cccagacccc aagaatggtt
     3721 acaaacaagg gatgtattga gctaaagggc accaacatcg atgtagtgca gataattcaa
     3781 aagggataga aatgcaagat ccaaatcctt tgccatgggg cgcacaagac cgttttcagg
     3841 ctcactttat agtcaaaggc aatgtgggaa gtaaagacct agattttgtt gcagaaacaa
     3901 aactaaaaac aaaaggtcat tttggctcaa aaacactggt tggagtagaa tgggttggag
     3961 gaaaacttgc agaagcactc aacggtgata gcgaacttgc aagcatgatt ctaaaacaac
     4021 cgataaacga tgctgccata tgggttgacc cgacaaaaaa tggtgtaaga atttatggca
     4081 aatggaaagc agatcatgaa cttggtatta caaaagacct gtttgctgtc tatgacagaa
     4141 ttgcatcgca cataaaaaaa gaactctagg ctctccacca atctaaagcc aaacaatcta
     4201 ccttgtcttt tccacgcggt attgccaaaa tgaattgttt tctgactgtt gttgccaatc
     4261 ttccggcaag tactactcct gtggcagtaa ttgtgtcact tgtattgtat acctgaacca
     4321 gatatggtgc gtgatcgata cctggtccct tttgatatac tgcaaaatca caaccaaatt
     4381 ttattcctgg atttacaata taccccttat ctctaaagtc cttgtagacc tggtattttt
     4441 tatcaaaatt gtgatattct tgtctgcata ttgctagcag atcgttttct gatatttgct
     4501 tttttgccct gtatactttg atctttgatt tttgtaatag gaaatatcct tctatcaaat
     4561 ccaaaatgag aggcacagtt atctcatctg gctttggttt ggtaatgcct atgggcttgc
     4621 catagtatcc ttgcatgaaa agagaacgcg aatcctgcac attccaaatt acgattcgat
     4681 tctcaattag ctcgcctttc ataacagaca gttacaacac gtggctaaat agataacttt
     4741 tccagaatct agttgctcaa acaaaaatta attctcttat ggtttggtaa agtttgcaaa
     4801 cttgccttcc catccttccg gtctcatgat atcagactct gatgggtcgt tgccaattgc
     4861 taatcttgca gctgactcat ccatgttttt gctttcaatc ttgtctatta ttgacactgc
     4921 attgttgatt ggtgtatcat caggtccaaa taaaaaatgc atcccaagat ctccaatgat
     4981 taccgcccca cctacaccaa ttgcaatcaa aagaaatgtc ttgtctttaa ttttcttcaa
     5041 tgctataggc ttaaggccaa tgcgataaaa gagtcagaaa cttcaaggca cttgtctgcc
     5101 atctcttcta ctccgccaac tacatctttt aaaagcaata attcctttat tgatgtaatt
     5161 tcatccagta caacaattgt aagttcacga tacttgtcat ctatgttacg ctctattttt
     5221 tgggcttcat gtgcaagctc gattgcacct gcaggatttc cacttagagc tcgtatgatc
     5281 tcgtttagct tgtagatttc atcgacaact agattgatta aatctgtaag gtctttgtca
     5341 agttttgcct ttttcagcgt cattgccttg atgtttgaaa gcttgaatgc aattcctgta
     5401 atgtagcctg caatctcatc cattgtgtat gcagtatgca gaaggttttc tctattcatc
     5461 atcagtccgc cgacatcggc aatttctctt gttatctttc ggcgaagagc ttctacctcg
     5521 tcttcggtgt ttcgcattct ttcaagagat gctttaattt ctgatttgtc accttttacg
     5581 ataagagcag gaagtgcaga gagatctctt acggcgttta agattctgtt tatttcatct
     5641 tgcaaaactg caagcgcctt tcgttttgct tgcacttcta attctccgct atacatgtaa
     5701 ggctttaaag tactaggagc gactaataat tcttacgtaa aattccatgt gttgataatt
     5761 tgatgagaca aacgacctga tttagatata gttttgtttt tcttcctgaa ctactctttt
     5821 ttgtccgttg tacaaagtaa ttatctcctg atctgttgtg gcgttttctg gagttttctc
     5881 aaatcttatc tttcctgtaa attttgggaa tcttagtgca agaccacttc cttttcttat
     5941 tgcatccatt gctgttttgt gcaatggact gagtgttatt tctgaagcta cgatctcaat
     6001 tacaacttct ggctcaaacc acacttcagc ttctagtccg ctgtttattc taggattttt
     6061 ctttaatgtt atcttgtcgg aaagtatctg gtagaactga tcaaggtttt cgtctgtaaa
     6121 tccggtgcca actttgcata tgcttggaaa tgtgtctgtc tgttcatcat atgttgcaag
     6181 caataacgct ccatatcttc cggttcttct gcctcttcca aaaaatgccc caattacaac
     6241 aagatccaga ctgtctccaa gctcgtttct gtattctcgt tttaatttta gccagttgct
     6301 gcctctggct cctgccctgt aaggtgaatc cagatgtttt agcatcacac cttcgcatcc
     6361 tacaataatt gcattttcta gcgcatcttc aacttcattg tccgtcttta caatagtcat
     6421 tggcattagc tttacaaaat tatcttcttt tacaattttt tcaagtatct ttctgcgttc
     6481 tctgtatggc aaatcaaggc agcttttgcc gtctgcaaac atgacatcaa aaaagtttat
     6541 cgttatagga tactgcgaga ctgctttttg tatgttgtat ttgcgtctgc gatgcatgag
     6601 ttcttgaaat gcaagaaatt ctcccgagtc ttcatttatt gcaactgcct ctgcctcaag
     6661 tattgtctca tgtgtgttca atgacttggt aactttttca acaatgtcag gatagtagct
     6721 tgtgatgttc tcaagacttc gagaatagag tacaactttt ccatcttgca agtgtagttg
     6781 aacgcgttct ccatccagct tgtattctgc agcaaactct tgtcccattt tttcatgcgc
     6841 ttcttgcgga ctctttactc gctcagctaa cattggcctg atgggactga acactgaaac
     6901 ttggaaactc tttactccat tgattccgtc ttttgccact gctgcagcaa ctttgccaag
     6961 atcacttgat acgttgtatg cctgctcaag gactgcacga ttttctttgg tacctgtaaa
     7021 ggcaactgcc agagaatcca ttatggtgaa atctgcaata cctagtcgta gagaatttgt
     7081 aattatcttt aggataaatc ctgattcaat cgggctggca tcatttaaca ggcttgaaat
     7141 gtactttagt ttcatgtcct gcgagcgtga acccttgagc tctgcaattt tgtataacgt
     7201 gtcgtaaact ctttccaccg tgatatcttc tgtcataaac gtggtctgcg tcttttgctc
     7261 caaaatcttt gcagcagcct gacccaaatc tcctgtttct ttgtatgctg tttctatctt
     7321 tttttgtgga attcctgcag attttgatat tgctttaatt gcaatttttt ctgcaacccc
     7381 aagctccact ccttcgtggt caggtctgag ttttccttgt aacaaataga ccacacttga
     7441 gattgtctct gggggtgttt ttttaaacaa atccacaaga tactgggtta gctccaacct
     7501 ctttgtggtg ctttccatta tcttcagagt ttctgctact agggaaaact gcatctgacg
     7561 atttctatcg attctacaat aaaagcttga gttattttat ctgaaggtgt gcctttatct
     7621 taagccatat ctcctgtgat ctttttaaaa acatggcatt aaagtcagac aactcgtcat
     7681 cgtttagaaa tctcttgttt cttgccaaaa acccatcaaa gaatattcct gatacatatc
     7741 ctacatagag gccgtatgca aagtctccaa gatttgctcc ttgcggtaca agtgatttta
     7801 cttcggacaa gaccacgcgc ggataatcaa cggcataagt tatgatgtcg tttagatttt
     7861 tttcttcgat gacatctagt cccatagtac aaccgagttt aagaaatatt taggattttt
     7921 tcgagtcttg ttcgtttata aataggacta aatgaaaagc cgacatgggt ggagcaaaga
     7981 aacaatctcc tgcacaagca gaaaaatctc agcaagccga ggctgcaaaa aaagaaggag
     8041 gctcaaagaa aggggagaag aaagagcaaa aatccaaaag ctcgatctct gtgatcatta
     8101 atgaaaatca agctctaaac tttatcaaaa actccaaagt tttcacagtg caagaaattg
     8161 caagacagga aggagtaaag atttctgcgg caaatacgtt tttgcaaaaa ttgctaaaaa
     8221 gtggctcagt taagagaata ggcggataca gcggacatca tatctacact catgcctgag
     8281 tgataacaaa aacatctcct gattttaatt cctttaatgc atcaatgttt gatgtaatgt
     8341 ttcctattgg agtcaatgcc tttgttgatt ctgaatcgtt tagaaaaaaa cagacagaac
     8401 cacctagtgg cataaacgca atctctcctt ttttgaattg atttctctgc ctttctccac
     8461 ccacgtttat cttggtttca acatatgcaa agctgtttcc catcatgtgc gcattgccct
     8521 ctattggcag ggatcttatc atggttccaa ccgtttttgg tgcaagatgg cgttttagct
     8581 cgcattttag ctgagatttt ccctttattt ctaaaataac ctcgattttt gaaacagatc
     8641 cggcgctcat tttcaagagg aggattatga attatataac gtttctttag acagaacgta
     8701 tccatgtctg acgagcctga agaaattatt cctgatactg aagaagttgc agtagagcca
     8761 gaagagccag aggttcctgt tgtcgaagaa gttgtagaaa caaccgaagc cgtaggcgaa
     8821 gaggaagaat taccagaaaa gaaaaagaag gcaaaaaaaa gtgatcttac tccagaagag
     8881 cttgcagaaa aagaaagact gcaaaaaata attgatgcta gagaaattat cgatgttgat
     8941 cctatacacg taccaattga gtttgaaaca acaggcagag gcaaaataat gataggcccg
     9001 cccactctaa caaggtttga aaaggcaaga ataatgggtg caagggcgct acaactctca
     9061 ctaggtgctc cgccattcat tcaggttcca aaagaagttg caacttcact tgatcttgct
     9121 tatgcagagc ttgaaaaacg ggtcattcca ataacaatta gaagagtatt cccaaatggc
     9181 gactttcaaa acatcccgat caatcaattt gattaatgga ccaatcgtcg ataagattta
     9241 aaagaaacta aattttcggt gatatgattg agtcttttga gtgaaataga tgtaaaaact
     9301 catcttgtta ataacccgtc attaccaaat acagggaaag tggtagtcat acaaagacag
     9361 ccaataatgg aatgcgcact tgacatattg cctatcatat caggccctgg tcgcgtcaca
     9421 cttagcgcca aaggagatga catccctact gcagttgggg tggcaaacgt gataactgaa
     9481 aacatgatga aaggaaatgc cgaaattgtt gatattattg tggacagcga aaacggacca
     9541 ggacaatacg gaatgactct gatctcaaca attcaaataa ttattgctag aaaattaaat
     9601 taaaaagaac ttcctggacc tttgagaagc atgttttcgc attctttgca tattgcttca
     9661 tcaatatttg tttcaagact gtgatgaatg tcgcatatca acaaacttga ttttatgtaa
     9721 ttgcacaaac ctatgaactt tgaaaggctt gcaggtgtgt atgccatgtt ggatgaaaga
     9781 ttgcttgcaa tctcatcaat catttctgaa acttctttta ttgcaagaag cttggtgata
     9841 agttcgggat cccccctggt tcctcggata tagttgctaa ttgcagcctg tgttactcca
     9901 agcatttttg aaatgtcttc ttcttggatt ttgtgctgtt ctacaagttt ttttgccaaa
     9961 attgctcgca gtgcaggaat gagtgttttt gtttcaattt cggcaggaag taacatgata
    10021 aaacaaggac tgcaaaaata tttaaaccta ccaataagct tgacctaatt ttctggaaca
    10081 aattttagtt gcataccgcg ataagttatt ttatcaacca attcatatca gcatattgca
    10141 agaaattttc tcaaatctgc aaaaacagat ctcagaagca gtatcgtcat gcaaatcaaa
    10201 ttgtatttcg ctgtcaggtg gactggatag ttccatactt gcctgttatg tggacaaaaa
    10261 gatcaaggca attgctgttg taacaaaaga ctttccagcc acggatctaa tgttttcaca
    10321 gcttgttgca aaacaatctg ggctggactt gatcataaaa aacgctacaa ttgaagagat
    10381 cttgtctgca attgaagaga caataaagat cctgaaagtt ttcaatcaca tcgagatacg
    10441 aaactct
//
LOCUS       DATIDL010000044         3358 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrosopumilaceae archaeon isolate SMAG_U16342
            k141_54383, whole genome shotgun sequence.
ACCESSION   DATIDL010000044 DATIDL010000000
VERSION     DATIDL010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935836
            Sequence Read Archive: SRR25158326
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosopumilaceae archaeon (soil metagenome)
  ORGANISM  Nitrosopumilaceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrosopumilales;
            Nitrosopumilaceae.
REFERENCE   1  (bases 1 to 3358)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3358)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/04/2024 05:09:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,520
            CDSs (total)                 :: 1,474
            Genes (coding)               :: 1,474
            CDSs (with protein)          :: 1,474
            Genes (RNA)                  :: 46
            rRNAs                        :: 1 (5S)
            complete rRNAs               :: 1 (5S)
            tRNAs                        :: 43
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3358
                     /organism="Nitrosopumilaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54383"
                     /isolate="SMAG_U16342"
                     /isolation_source="soil"
                     /db_xref="taxon:2202732"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..526)
                     /locus_tag="VLB45_01960"
     CDS             complement(<1..526)
                     /locus_tag="VLB45_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HSD04503.1"
                     /translation="MTFPNVRLRRLRESKKIRELLEEVRLSPKDLICPVFVQEDLKSK
                     VQVDSMPDIERLPLKDVVNEVNTIADLKIPAIMLFGIPSSKDDIGTSAFAENGIVQKA
                     ISEIRKNLGNEIAIMADVCLCQYTSSGHCGLVKGDKIDNDSSLDVLKKIAVSQAQVGV
                     DVVSPSAMMDGQVKA"
     gene            complement(563..1819)
                     /gene="hemA"
                     /locus_tag="VLB45_01965"
     CDS             complement(563..1819)
                     /gene="hemA"
                     /locus_tag="VLB45_01965"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HSD04504.1"
                     /translation="MSLNVINARVTFRKSPIHMLERFTFRDVDSAYSSFIKHAGLQEC
                     VILQTCNRVEVFGAGSSKDIEKIKKTWASVSGLEENAFKENLEVSEGTEAYEHLLKLT
                     AGLDSLVVGEEQILGQIKESISAAKEVKASGNNLNTLFEKAIRVGSRVRSSTGISRGS
                     ISIGSMAVKLAEENIDDLKSKRILLIGTGEAASLVAKSLKRRNMEFFVTSRTFDRSKA
                     FSETVGGKPLKFEDTLANFKTVDVLFVATVAPYFLVTYDRVKEAMESKKTGMMILDLS
                     NPRTVDEKVATIGRIKMFNLDQIAEMVDKNMRNRIGVVSSAEKIISEEMPVLEAVMKR
                     LEVEPVVKNVFKDIDSVRVKELRKALQMLGETDEEKIRIIDQLTQAIVEGIISTPMNN
                     LRRASEQGDSELLKSVSKLFDYNTKE"
     gene            complement(1816..2463)
                     /locus_tag="VLB45_01970"
     CDS             complement(1816..2463)
                     /locus_tag="VLB45_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010195434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HSD04505.1"
                     /translation="MDLNLKGKLVIVVGGGQEGLKKINSLLTQDCKILLVSDKTNRQI
                     DDYVKKKKIVFKKMKLENADFLSKYKPILVMAATDDKKLNRKIVERAKKMRCYAYAAD
                     DPAVSDFSHPSVINIEDTVQIAVSTGGRSPAMARRIRMQAEKVFKKIVKKEDIDHIRL
                     QEVARSLAKSKLATPLERKKFLYQIINDSKVKQLIKDDNFKKAQKRVMEMLRNHK"
     gene            2559..>3358
                     /locus_tag="VLB45_01975"
     CDS             2559..>3358
                     /locus_tag="VLB45_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007401217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HSD04506.1"
                     /translation="MDSLDKEILNEIQWTFPLVSQPYTEIAKKFKISQEELTKRLVEL
                     KKKGVIRQLSAIFDTRKLGYKSALIAMSIAPEKLDYVASQINKHPGVSHNYERNHEYN
                     LWFTLAVPPGSDLKTEVDKFSKLSGIKKVRLLPTIKLFKIGVKLDMVEESAAEIKPTE
                     EKKKILDVKFEATEEDKEYIRELQKDLEVVPRPFLKSAQNLGITEEQLLEKAKYYEEI
                     GVMRRFAAILRHRDAGFVANGMIVWKVPEEKIDETGAKLGAFPQVSHC"
BASE COUNT          900 a          734 c          613 g         1111 t
ORIGIN      
        1 ttgcctttac ctgaccgtcc atcatggcag atggtgatac cacgtcaact ccaacttgag
       61 cttggcttac tgcaattttt tttaaaacat ccaaactgga atcattgtct atcttgtctc
      121 cttttacaag gccgcagtgt cctgagctgg tatattgaca aagacaaaca tctgccatta
      181 ttgctatttc gtttcccaaa ttctttctga tttccgaaat tgccttttgc actatgccgt
      241 tttctgcaaa agctgatgtc ccgatatcat ctttagatga tggaatgcca aacagcatga
      301 ttgcagggat ttttagatca gctattgtat tcacttcgtt taccacatct tttagcggca
      361 gcctctctat gtcaggcatc gaatcaacct gcactttgga tttcaggtct tcttgaacaa
      421 acactggaca gattagatct tttggagata gtctgacttc ttctaaaagc tcgcgaatct
      481 ttttactttc ccttaaccgt cttaatctta catttggaaa agtcatattg aaaaaacact
      541 gcctgctagc aaatataatc tgttactctt ttgtgttata atcaaaaagc ttgctgacgc
      601 ttttgagcag ctcagaatcg ccctgctctg atgcccgtct caaattgttc attggtgtag
      661 atatgattcc ctccactatt gcctgtgtta gctggtctat gattcgtatc ttttcttcat
      721 cagtttcgcc aagcatctgg agcgcctttc tgagctcttt taccctgact gaatcaatgt
      781 ccttgaatac gttttttaca actggttcta cttccaatct cttcattacc gcctctaata
      841 ctggcatctc ttcactaatt attttttccg cagacgatac cacgccaatt ctgtttctca
      901 tgttcttgtc aaccatctct gctatctgat ccaagttgaa catctttatt cttccaattg
      961 ttgcaacttt ttcgtcaact gttcttgggt ttgacaaatc aagtatcatc attcctgtct
     1021 tttttgattc cattgcctct tttactctgt catatgtgac aagaaaatat ggagcaactg
     1081 ttgcaacaaa caagacatca actgtcttga aatttgcaag cgtgtcttca aacttgagtg
     1141 gctttccacc taccgtttct gagaatgcct ttgatctgtc aaaggttctg cttgttacaa
     1201 aaaactccat gtttcttctt ttgagtgatt ttgcaacaag gcttgcagct tcacctgtgc
     1261 ctatgagcag gatgcgcttt gattttaaat cgtcaatgtt ttcttctgcc agcttgactg
     1321 ccatagaacc aattgaaatg cttccgcggc ttattccagt gctgcttctt acgcgactgc
     1381 ccacgcgaat tgccttttca aaaagtgtat tcaaattgtt tcctgatgct ttaacctctt
     1441 ttgctgcact gatggattcc tttatctggc ccagtatctg ctcttcgcca acaacaagtg
     1501 aatcaagccc ggcggtcaat ttcaaaagat gctcgtatgc ctctgttcct tcacttacct
     1561 ccaggttttc tttaaaggcg ttttcttcta atcctgagac tgatgcccaa gtctttttta
     1621 ttttttcaat atccttgctg cttccggcac caaagacctc gactctgttg catgtctgta
     1681 atatgacaca ctcttgcaat ccggcgtgtt tgataaatga agaatacgcc gagtcaacat
     1741 ccctaaacgt gaatctttct agcatatgga ttggcgattt acgaaaagta acccttgcat
     1801 taatcacatt aaggctcatt tgtgattcct caacatttcc atgactcgct tctgtgcttt
     1861 tttgaaatta tcgtctttta ttaactgttt gaccttgcta tcgtttatga tttgatacag
     1921 gaatttttta cgttcaagag gagttgcaag cttggatttt gccaacgacc ttgccacctc
     1981 ctggagtctt atgtgatcaa tgtcttcttt tttgacaatt tttttaaaga ccttttctgc
     2041 ctgcattctg attcttcttg ccatggcagg gctcctacct ccagttgata ctgcaatctg
     2101 cactgtatct tcaatattga tgacagaagg atgggaaaaa tcactgactg caggatcatc
     2161 agctgcataa gcgtagcatc gcatcttttt tgccctctct acaatttttc ggtttaattt
     2221 tttatcgtct gttgcagcca tgacaagaat tggtttatac tttgagagaa aatctgcgtt
     2281 ttcaagtttc atttttttaa aaactatttt tttctttttg acataatcat caatctgcct
     2341 gttggttttg tcgctgacaa gcaatatctt gcaatcctgt gttagaagcg aatttatctt
     2401 tttgagccct tcctgtccgc ctccaacaac tatgactagt tttcccttca ggttaagatc
     2461 cactatcaat gattctggct ctctttgaat gatatttata gattgcaaga aactcggtac
     2521 attttttaat tgcacggcgt ttttcatacc taatgtcgat ggatagtctg gataaagaaa
     2581 ttctaaacga gatccaatgg acgtttccgc tagtttcgca gccttacact gaaattgcca
     2641 aaaaatttaa aatttctcaa gaagagctta caaaacgact ggttgagcta aaaaagaaag
     2701 gcgtcataag acaactaagt gcaatctttg acacaagaaa gcttggttac aagagcgcgt
     2761 tgattgcaat gtcaatagcg ccagaaaaac tagactatgt tgcaagccag ataaacaagc
     2821 atcctggagt aagccacaac tatgaacgaa accatgagta caatctctgg tttacacttg
     2881 ctgttcctcc aggcagtgat ctaaaaaccg aggttgacaa gttttcaaaa ttgtctggaa
     2941 tcaaaaaggt aaggctgctt cctacaatca aactcttcaa gattggagta aagctggaca
     3001 tggtagaaga aagtgctgca gaaataaaac ctacagaaga gaagaaaaaa attcttgatg
     3061 taaagtttga agctaccgaa gaagacaagg agtatatccg tgagctgcaa aaagacttgg
     3121 aagttgtacc tcgcccattt ttaaagtcgg cacaaaatct tggcatcaca gaagagcagc
     3181 ttcttgaaaa ggcaaaatat tatgaggaaa ttggggtaat gcgaagattt gctgcaatac
     3241 tgcgacacag ggatgcagga tttgttgcaa acggcatgat tgtctggaag gtccctgaag
     3301 aaaaaatcga tgagacaggg gcaaaactag gcgcgtttcc gcaggtaagc cactgcta
//
LOCUS       DATIDL010000045         4388 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrosopumilaceae archaeon isolate SMAG_U16342
            k141_54480, whole genome shotgun sequence.
ACCESSION   DATIDL010000045 DATIDL010000000
VERSION     DATIDL010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935836
            Sequence Read Archive: SRR25158326
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosopumilaceae archaeon (soil metagenome)
  ORGANISM  Nitrosopumilaceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrosopumilales;
            Nitrosopumilaceae.
REFERENCE   1  (bases 1 to 4388)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4388)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/04/2024 05:09:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,520
            CDSs (total)                 :: 1,474
            Genes (coding)               :: 1,474
            CDSs (with protein)          :: 1,474
            Genes (RNA)                  :: 46
            rRNAs                        :: 1 (5S)
            complete rRNAs               :: 1 (5S)
            tRNAs                        :: 43
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4388
                     /organism="Nitrosopumilaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54480"
                     /isolate="SMAG_U16342"
                     /isolation_source="soil"
                     /db_xref="taxon:2202732"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            2..793
                     /locus_tag="VLB45_01980"
     CDS             2..793
                     /locus_tag="VLB45_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HSD04507.1"
                     /translation="MISGIAIALMCSVVGLFLVLRRYSLFGDALAHAAFGGVAAGLFL
                     GIYPIWAAFIVSISSALGLTKIRQKFQISGDATVAILLSSGLAVGVVLISLAGGFTVD
                     IFSFLFGSILLVSLENTILILGLAGSILIVILLLYRQLVYATFNEEQAKVSGIPVEKL
                     NYLLVGIAGLTVVSSMQLVGVLLISSLIVIPNVTAIMFGRGFKHTAILSMIFAVSSVV
                     VGILTSYSFNIAPGGMIVLISIVIFVVSLGIKSAGLIKNKTLIKA"
     gene            complement(801..3569)
                     /locus_tag="VLB45_01985"
     CDS             complement(801..3569)
                     /locus_tag="VLB45_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HSD04508.1"
                     /translation="MKKVLLVVIIACCFIFGLAEAHPFIEDSEPKQSSNVPPGITQII
                     IYYSEAVEMDYSTIKVLDNKGTQVDNKDTKYFQGENSLVVTTRPLDDGVYTVTSKVLS
                     KVDGHIVDDALIFSVGSAKIDPSLLNKKSEQPSVYIPEALARFPGLVGQVMILGAVIS
                     SLLIWRSLRTKQAIKENLELQKKHSHLFLKLTGVGMLLVLGSNIAMLAVQSIALQTSA
                     LEAIQTGFGTTWLVRMSLTIILFAIWFWAERKAMVGKVQQFLILGFSLALIATTTMLG
                     HGAATMHESAIILDYTHNLLASVWIGGVIYAAFAMIPSFEKMESDKRERLSLLMIPKL
                     SSMIIVAVGILLITGPTLLWFLESDVGLLYDSTYGKLILAKIALGIILIVIGGYNQFR
                     IQKPAEKNLQSGTISVYKKFRKSLKVESIIGIVLLGVVALLANSSLPAGEIQQVQALD
                     VNGYQTIQFSSGARFDVSIEPLSSGTNTVSVSVMSLDGKSLGDVSEVVMKVSNPQKGI
                     SPIESNMSPIKSADSDSQVTQYVGNATFGFTGTWHVEIVALRTQTANDAISFDVLIKP
                     RLSELKTSMVEYGFPDADVAPLYPVFDGTNTIWISDTAKPRIWKFTIDDKQFKSYEFD
                     GQISVTLNIDNDGKIWYTDTPNSRIGFFDPDTEKFENIDLPIKSVPIFTETDLQNNIW
                     VALADKNILLKYNQEKKEFSEFKISQAPLSGPTALILDETGNIWFTEAQAGKIGVINP
                     QTGQTREFTPEESLKEPTALFFDDNGNLWISEHTGPTITRFDPILETFERVPVLDPDA
                     LPFGMTSDKYGNIWIGQHTVDSLGVYDPYNGNFNEIPIPTQQSFVQFITSDDDGNIWI
                     VEQRGQKLGMVSITEMPRQIAPSTGGEFQIKYVELVSPLISAGIIATSLFFVQSIKDK
                     RRINSLVD"
     gene            3671..4309
                     /locus_tag="VLB45_01990"
     CDS             3671..4309
                     /locus_tag="VLB45_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007551519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04509.1"
                     /translation="MQKLSLVLFVLSLSTIFGISVAYAHTTVEVETLEIEVGWQDEPP
                     LVGFMNAITFEVNENAVDGQSGVKNAFKNLVATVKSGGITKTLDIDSDITAGHYHSKI
                     IPTRTGNLVVELKGDINGIPVNSETTIEAVEDKSLLAFPDTTGSSDQDVTALKNAMSA
                     LQTEVTTLKSKMTGIDTDGNFDVETAYNFGIFGLGLGAAGVILAIIAMIKRK"
BASE COUNT         1291 a          817 c          836 g         1444 t
ORIGIN      
        1 aatcatttca ggcattgcca tagccctcat gtgttcggtt gtggggcttt ttttggttct
       61 gcgaagatat tcactttttg gagatgcgct tgcacatgca gcttttggtg gtgtagctgc
      121 tggattattt cttggaattt acccaatatg ggctgctttc atagtttcaa tatcaagcgc
      181 acttggtcta acaaagatca gacaaaaatt tcagatctca ggtgatgcta ccgttgccat
      241 cctactctcg tccggacttg ctgtgggagt tgttcttatc agtttagcag ggggatttac
      301 tgtagatata ttcagctttc tttttggaag cattttgttg gtaagtttgg aaaatacaat
      361 tttgatttta ggtcttgcag gatcgattct aattgtaata ttgttgttgt atagacagct
      421 agtctatgct acatttaatg aagaacaggc aaaagtaagt ggcattccag ttgaaaagct
      481 aaattattta cttgtaggaa ttgctggctt gactgtagtg agctcaatgc agcttgtagg
      541 agtcttgtta atttcatcac ttattgtaat accaaatgtc actgcaatca tgtttggacg
      601 tggattcaag catactgcaa ttttgtcaat gatttttgca gtctcttctg ttgtagtagg
      661 aatattgaca tcttatagtt tcaatattgc accaggaggt atgatagtgt taatttctat
      721 agtgatcttt gttgtatcac ttggaataaa atctgcagga ttgatcaaaa acaagacact
      781 catcaaagcg taattaattt ctagtctacc aaactgttaa ttcttctttt gtccttgatg
      841 ctttgcacaa agaacaatga agttgcaata attccagcag aaatcaacgg tgaaacaagt
      901 tcgacatatt ttatttgaaa ttccccacct gtagatggtg ctatctgtct tggcatttca
      961 gttattgaaa ccatcccaag tttctgacca cgctgctcta caatccaaat atttccatca
     1021 tcatctgagg tgataaactg tacaaatgat tgctgggttg ggattggaat ctcattgaaa
     1081 ttcccattat atgggtcata aactcccaaa ctatcaacag tgtgctgtcc aatccaaatg
     1141 ttaccatact tgtcagatgt cataccaaaa ggtagtgcgt ctggatctag aaccggaact
     1201 ctctcaaaag tttcaagaat tggatcaaat ctagtaatgg taggtcctgt gtgctcagaa
     1261 atccacagat tcccattatc gtcaaagaaa agtgcagtag gttctttgag agattcttct
     1321 ggtgtaaatt ctcttgtttg gccagtctgt gggttgatga ctcctatctt tcctgcttga
     1381 gcttcagtaa accagatgtt tcctgtttca tctaagatca gtgcagtagg accagaaagt
     1441 ggagcttgtg aaatcttaaa ctcggaaaat tccttctttt cttgattata ctttaacaga
     1501 atgttcttgt ctgccagtgc aacccaaatg ttgttttgca aatcagtttc tgtaaatatt
     1561 ggtacagact ttattggaag atcaatgttt tcaaattttt ccgtatctgg atcaaagaaa
     1621 ccaattcttg aatttggcgt atcagtgtac catatttttc catcgttatc gatattcagg
     1681 gttacggaga tctgcccatc aaactcgtat gatttgaatt gcttgtcatc aatggtaaac
     1741 ttccatatgc gtggtttagc tgtatcactt atccatattg tatttgttcc atcaaatact
     1801 ggataaagcg gtgcaacatc cgcatcagga aaaccatatt caaccatgct tgttttgagt
     1861 tcagaaagtc ttggttttat caggacatca aacgatattg catcatttgc tgtttgtgtt
     1921 cgcaaagcca caatttctac atgccatgta cctgtaaatc caaacgttgc attgcctaca
     1981 tactgcgtta cttgggaatc tgaatctgca gattttattg gcgacatatt ggattctatt
     2041 ggagaaatac ctttttgcgg gtttgatact ttcattacta cttcagatac atcgccaagt
     2101 gatttaccgt caagagacat tactgataca gaaactgtgt ttgttccact tgaaagcggc
     2161 tctattgata catcaaatct agctccactt gagaattgta ttgtctggta accattgaca
     2221 tctagagctt gcacttgctg aatctctcct gcaggaaggg agctgtttgc taataatgca
     2281 acaactccaa gaagcacaat cccaataatt gattccactt tgagagattt tctaaatttt
     2341 ttataaactg atatggtgcc tgattggaga tttttttctg caggtttctg gattctaaac
     2401 tggttatacc ctcctatcac aatcaatatg attccaagtg caatttttgc aagaattaat
     2461 ttaccatacg tggaatcata gagtagtcca acatcacttt ccaagaacca tagtaatgtg
     2521 ggtcccgtga tcaacaagat tccaactgca actattatca tgcttgaaag ctttggtatc
     2581 attagcaacg acaatcgttc tcttttgtca gattccatct tctcaaaaga aggaatcatg
     2641 gcaaatgcag cataaatcac gccacctatc cagacggaag caagaagatt gtgtgtataa
     2701 tctagtatga tggcagactc atgcatggtt gcagctccat gcccaagcat tgttgtagtt
     2761 gcaatcaacg caagtgaaaa accaagaatt aggaattgtt gcactttgcc aaccatcgct
     2821 tttctttctg cccagaacca tattgcaaac aagattatgg taagtgacat tcttacaagc
     2881 caagttgtac caaatcctgt ttgtatggcc tcaagtgcag aggtttgtag tgcaattgac
     2941 tgaaccgcaa gcatggcaat attggagcca agaaccaaaa gcattccaac tcccgttaat
     3001 tttaaaaata gatgagaatg ctttttctgt agctccaagt tttcctttat ggcttgtttt
     3061 gttctcagtg atctccagat cagtaaagat gagataactg cgcctagtat cattacttgc
     3121 cctacaaggc ctggaaatct tgccaaggct tctggaatgt atactgatgg ctgttctgat
     3181 tttttattca agagggatgg atctattttt gcactgccaa cagaaaatat caaagcatca
     3241 tctacaatat gcccatcgac tttggaaagc actttgcttg ttacagtata gaccccatca
     3301 tcaagtggtc tagttgtaac aacaagcgag ttttcgcctt ggaaatattt tgtgtccttg
     3361 ttgtctactt gtgttccttt gttatctaaa actttgattg tactataatc catttcaact
     3421 gcttcagaat aataaattat aatctgggtt attccaggag gaacattgga agattgtttt
     3481 ggttcactgt cttctataaa cggatgagct tctgccaatc caaaaataaa acaacaagca
     3541 ataataacaa caagtagaac ttttttcatt taataactaa aatcacatat gataatttaa
     3601 acacttgatt actttgatgt ttgtaccaca cctgcgtaaa gataataaca tgtaagactt
     3661 aattcaactt gtgcaaaaac tttctcttgt attgtttgtt ttgtctttat caactatttt
     3721 tggaatttcc gtagcctatg cacatactac agttgaagtg gaaacccttg agattgaggt
     3781 aggctggcag gacgaaccgc cactagttgg ctttatgaac gcaattactt ttgaagtaaa
     3841 tgaaaatgct gttgatgggc aaagcggtgt caaaaacgca ttcaaaaatc tagtggctac
     3901 cgtaaaatct ggaggaataa caaaaacact tgacattgac tctgatatca cagcgggaca
     3961 ttatcattct aagataattc cgacacgaac tggaaatctt gttgtagaac taaaaggtga
     4021 tattaatgga attccggtaa attctgaaac tacaatcgag gctgtagaag acaaatcact
     4081 acttgcattt cctgacacaa caggttcatc agatcaggat gttactgcat taaagaatgc
     4141 catgtcagcg ttacaaaccg aagttactac attaaagtca aagatgacag gaattgatac
     4201 tgacggtaac tttgatgtag aaactgctta taattttgga atatttggac ttggtcttgg
     4261 agcagctggc gtaatcttgg cgataattgc catgataaaa agaaaataaa ggtttagagt
     4321 tttggaataa ctctagtttt tgcagttact gctatgatgc ttactatgct tattgcaagc
     4381 actagtgc
//
LOCUS       DATIDL010000046         2808 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrosopumilaceae archaeon isolate SMAG_U16342
            k141_2276790, whole genome shotgun sequence.
ACCESSION   DATIDL010000046 DATIDL010000000
VERSION     DATIDL010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935836
            Sequence Read Archive: SRR25158326
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosopumilaceae archaeon (soil metagenome)
  ORGANISM  Nitrosopumilaceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrosopumilales;
            Nitrosopumilaceae.
REFERENCE   1  (bases 1 to 2808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2808)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/04/2024 05:09:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,520
            CDSs (total)                 :: 1,474
            Genes (coding)               :: 1,474
            CDSs (with protein)          :: 1,474
            Genes (RNA)                  :: 46
            rRNAs                        :: 1 (5S)
            complete rRNAs               :: 1 (5S)
            tRNAs                        :: 43
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2808
                     /organism="Nitrosopumilaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2276790"
                     /isolate="SMAG_U16342"
                     /isolation_source="soil"
                     /db_xref="taxon:2202732"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(52..480)
                     /locus_tag="VLB45_01995"
     CDS             complement(52..480)
                     /locus_tag="VLB45_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007550276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease domain-containing protein"
                     /protein_id="HSD04510.1"
                     /translation="MKINEIQDSDYADAKITYVGFVDPYGVEGILILRADDGKEFHMR
                     AFSGEVARHITNFIGGQRDSIPTIYNMIEEVAELNELLLVRIKVYESGSVLRANLYFT
                     GKTELVMRNYRASDAIALAAFYNVPILVRKSLLKEKTDEN"
     gene            568..1392
                     /locus_tag="VLB45_02000"
     CDS             568..1392
                     /locus_tag="VLB45_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04511.1"
                     /translation="MEKQVTMQSSSRMMIIVGFVSIAISFLIYSKYDYDIAITPDAVP
                     FLERLAVGVYAVLFLSFGAIAYGLYRFYKSKVAKSDDTILSIIANSINNKRSKQVFVA
                     SAIGYGIFFSLTSGILVYQPEVIFSQHYGATVPSIHLNPCCGPVGYMPTIVGYVTEHV
                     GFKIIPVNLVLQIAVSFLVGLNFAIATKAFSMYRKGGGMGGFGAVTGLFIACPTCAGT
                     LLATLVGTGVGAFTIALTQLQTVFIVVSIPVLLATPVIMARNIRRFQTGCAVDSKP"
     gene            complement(1382..1636)
                     /locus_tag="VLB45_02005"
     CDS             complement(1382..1636)
                     /locus_tag="VLB45_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007401504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04512.1"
                     /translation="MDSCDTRTHAYKNGKTFDQCRQIAETITSQFAKKIEQDGKVSWK
                     EILETVDHDELVYKLTLKYLRRDGYNIGDWKTPEVKKTTV"
     gene            complement(1844..2695)
                     /locus_tag="VLB45_02010"
     CDS             complement(1844..2695)
                     /locus_tag="VLB45_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010193927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HSD04513.1"
                     /translation="MIPINPLQILFWTFRRSEEDVVNLYDSLLPLMQLATGGDMLNFG
                     YWDETAKTPLEAQQKLCSMVGNLAELSSANTVADLGSGLSAPAVYWKAQYNLSHITCL
                     NINFKQLKNSTKSLDSKISFVNATSTALPFASNSVDRVIALESAQHFKPLKRFIQESK
                     RILKQDGLFVIAIPVSTNGTSLLSLFKLGILSLTWSSEHYTLEEIRSTVLQGGFHIKE
                     VQTIGNRVYEPLADYYINNREKLRAKILKEYPSYLENILFKSLLKMKEVSKNKTIDYA
                     LIKCNPA"
BASE COUNT          773 a          549 c          551 g          935 t
ORIGIN      
        1 aatggagcta aaaaaagctc agcaagaagg aagactggaa gaaattgtta cctagttttc
       61 atctgtcttt tctttaagca gactctttct tacaagaatt gggacattgt aaaatgctgc
      121 aagagcaatg gcatctgatg ctctgtagtt tcgcatgaca agctccgttt ttccagtaaa
      181 gtaaagattt gcccgcaaga cacttccgct ttcgtagact ttgattctca ccaaaagtaa
      241 ctcgttaagc tctgcaactt cttctatcat gttgtagatt gtaggaatgg aatctcgttg
      301 gcctccaatg aaatttgtta tatgtctggc tacctctcca gaaaaggctc tcatgtggaa
      361 ctcttttcca tcatctgctc ttagaattag tatgccctca actccgtagg gatcaacaaa
      421 gcctacgtat gttatcttgg catcagcata atcagagtct tggatttcgt tgatcttcac
      481 gtttaactat cagagtcatc cattgcagat aaagcctttg gcaaacacag atacaattta
      541 atgttacaaa agttgagttt gtttttgatg gaaaagcaag taacaatgca atcatcgtcg
      601 cggatgatga ttattgtagg atttgtttct attgcaattt cgtttctgat ctattctaaa
      661 tatgattatg atatagcaat cactcctgat gcagtgccat ttttggaaag attggctgtt
      721 ggagtgtatg cggtgctctt tttgtcattt ggtgcaatcg catatggcct gtatcgattt
      781 tacaaatcaa aagttgcaaa aagcgatgac accatattat caataattgc aaactcgata
      841 aacaacaaga gatcaaaaca agtctttgtt gcaagtgcga taggttatgg aatatttttt
      901 tcactgactt cgggaatcct agtttatcag cctgaagtga tattttcgca gcactatgga
      961 gctactgttc catcaataca cctcaaccca tgctgtggtc ctgtaggata catgccaaca
     1021 attgtaggat atgtcaccga acatgttggt tttaagatca ttccagtaaa tcttgtcttg
     1081 cagatagcag tctcctttct ggtaggacta aactttgcaa ttgctacaaa ggcattctca
     1141 atgtatagaa aaggtggggg aatggggggt tttggtgcag taactggact ctttatcgca
     1201 tgtcctacat gcgcaggtac tctacttgct actttggttg gaacaggtgt gggagcattt
     1261 acaattgcac tcacacagct tcaaactgta tttatcgtag tatcaatccc agttctgctt
     1321 gcaactcctg taataatggc aagaaacatc aggagatttc agactggatg cgcagtagat
     1381 tctaaaccgt agtctttttc acttcgggtg ttttccagtc gcctatgttg tatccatcac
     1441 gtctgagata ctttagggtg agcttgtaga caagctcgtc atgatcaact gtttctagaa
     1501 tttctttcca ggacactttg ccgtcttgtt ctattttttt tgcaaactgg ctagtgatgg
     1561 tttctgcaat ctgtctgcac tggtcaaagg tcttgccgtt tttgtatgca tgggttcttg
     1621 tgtcacagct gtccatggga aaacttgaat gccgtacaat aaaaagatct ttgccaaatt
     1681 gagcatttta taataaaaca atccggaaac gtgttaaacg tcgcatacct tcgtacgcta
     1741 cctttcaggg ttattacccc gcgtttccgg catgtatcat aacgtgattt gaatatttaa
     1801 aatggtgatg taattcccag cattgataat cgcattttaa aatttaggct ggattgcact
     1861 ttatgagcgc ataatctatg gtcttgtttt ttgacacttc tttcattttc aataatgact
     1921 tgaaaagaat gttctcaaga taggaagggt attcttttaa aatcttggct ctcagttttt
     1981 ccctgttgtt gatgtaataa tctgctaatg gttcatagac acggtttcct attgtttgaa
     2041 cttcttttat gtgaaaacct ccctgcagaa ctgttgatct gatctcctcc aaagtgtaat
     2101 gctctgatga ccacgtcaat gaaagaattc ctaatttgaa caacgacagt agcgatgtac
     2161 catttgtgct gactggaatt gcaattacaa acaatccgtc ctgctttaag atccgctttg
     2221 attcctgaat gaacctcttt agtggtttga aatgctgtgc agattctaac gcaatcaccc
     2281 tatccacact attgcttgca aatggtaatg ctgttgaggt tgcatttaca aatgaaattt
     2341 tactgtctag agattttgta gaatttttta gttgtttgaa attgatattc agacatgtga
     2401 tgtgggacag attgtattgc gctttccagt aaaccgcagg tgcagacaac ccgcttccta
     2461 aatctgctac tgtatttgca gaactcagct ccgcaagatt tccaaccata gaacacagct
     2521 tttgttgtgc ctccaaaggt gtcttggcag tctcatccca gtatccaaaa ttgagcatgt
     2581 cgcctccggt tgcaagttgc atgagaggca agagcgaatc atagagattt acaacatctt
     2641 cttcactgcg cctgaatgtc caaaaaagaa tctgcaatgg atttattgga atcaatatta
     2701 tctctaagat tgttgttgat atatagtcat atgactagat tcatttcttg ctgatttttt
     2761 taacaaactc tattattccg gtgtaatcaa gttcgccaaa acctccat
//
LOCUS       DATIDL010000047         4623 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrosopumilaceae archaeon isolate SMAG_U16342
            k141_863983, whole genome shotgun sequence.
ACCESSION   DATIDL010000047 DATIDL010000000
VERSION     DATIDL010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935836
            Sequence Read Archive: SRR25158326
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosopumilaceae archaeon (soil metagenome)
  ORGANISM  Nitrosopumilaceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrosopumilales;
            Nitrosopumilaceae.
REFERENCE   1  (bases 1 to 4623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/04/2024 05:09:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,520
            CDSs (total)                 :: 1,474
            Genes (coding)               :: 1,474
            CDSs (with protein)          :: 1,474
            Genes (RNA)                  :: 46
            rRNAs                        :: 1 (5S)
            complete rRNAs               :: 1 (5S)
            tRNAs                        :: 43
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4623
                     /organism="Nitrosopumilaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_863983"
                     /isolate="SMAG_U16342"
                     /isolation_source="soil"
                     /db_xref="taxon:2202732"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..950
                     /locus_tag="VLB45_02015"
     CDS             <1..950
                     /locus_tag="VLB45_02015"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HSD04514.1"
                     /translation="GKIICLPSSTKFSMHGLFESGVREKFPKVSKNSCGYRIDQVTNK
                     SDSQKIIAGSEGTLGIILSAKLKTFPIKRKITLSILAYKTINQAVADVVKIIGLKPSA
                     LEIVDHNIAKNIDVRLARNTECLLFAEFDDNIRKNTYHLKNITHGKIIKTFVKNHEIK
                     KWWDFRNTALSYSLRSINPKQSMPTIIEDAVVPVENLGLLVDIIKKISKKYRLRFVLY
                     GHAGNGNLHVRPILGHKNKKIMKKIASEYFDKIIGIGGSITGEHGDGFARSEFVRQQY
                     GDDVYSVFKKIKNSFDPKNILNPGKIITHKTTVVKNLRM"
     gene            complement(951..1277)
                     /locus_tag="VLB45_02020"
     CDS             complement(951..1277)
                     /locus_tag="VLB45_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008299122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HSD04515.1"
                     /translation="MTNITYDDFAKLDLRIAKIVSTEKIPGKSKIIKGVIDLGEEKRE
                     VIIGGAEYYQPEELVGKTVVVIANLEPRKIAGLESNAMLLAADVNDRPFWLTVSEDVP
                     LGTKIK"
     gene            complement(1419..1559)
                     /locus_tag="VLB45_02025"
     CDS             complement(1419..1559)
                     /locus_tag="VLB45_02025"
                     /inference="COORDINATES: protein motif:HMM:TIGR04272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CxxC-x17-CxxC domain-containing protein"
                     /protein_id="HSD04516.1"
                     /translation="MSTDRQMHPATCADCKKETQVPFLPKEGRPVYCRECLPKHRGER
                     RF"
     gene            1682..2101
                     /locus_tag="VLB45_02030"
     CDS             1682..2101
                     /locus_tag="VLB45_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007550060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HSD04517.1"
                     /translation="MLAIIEASVSIRAPVDKVWNIISDLDNEPKFWKGTKEVRNISKD
                     GNTVTREVTIAFKDSKCMQTVTLTPKESIHTIFTKGIINGTKIVSLTAQQNETLLKAV
                     WDIKLSGMMGMFTGMVKKHIQSGTEQALEGIKQEAER"
     gene            2105..3313
                     /locus_tag="VLB45_02035"
     CDS             2105..3313
                     /locus_tag="VLB45_02035"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HSD04518.1"
                     /translation="MEFALDVFNYILSVIMIGVSITWILLIRSMWSSFRDSPFLDRFD
                     AKPHSNPKVSVILPARNEERFIEKCLNSLLNQDYQNYEIIVIDDRSEDNTGNIIKKIS
                     EKNSKIIHVTAQPKPERWIGKNWACFEGFKKSTGELLLFTDADTKHSRSMISLAVSHL
                     HSESLDALTVIPKMLCLDRWTKITLPVLSTFLHTRFSALRVNDPSKKTGYFFGSFFII
                     KRETYESVGTHEGVKGELVEDGALGKKVKESGFKMKMVRGEHLLEAIWARDFTTLWNG
                     LKRLVIPLYLQSKKIAVGIFFAVLFLLFMPFPILAYSSIFVSMAQSFSVLFLISIVTT
                     SLLYFAAVLEAKKGLGLSTIHALCGPVGSFIIVAGFGSGILQATSNTSVTWRGRTYSM
                     VDQVQNSISV"
     gene            3380..4543
                     /locus_tag="VLB45_02040"
     CDS             3380..4543
                     /locus_tag="VLB45_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HSD04519.1"
                     /translation="MDKTSTVFGGVIGGIIVVIVFALYFAPDGVSAPPKDIVTSNGHD
                     LETVGEVTSIPKNFDEITLADLFDKSDDGVVKVSVRKSLETPRSSSLGSGFVYDVNGH
                     IITNNHVVDDAQKITVTFLDGTSYNADVIGTDPFTDLAVIKVDVDPALLHPLPIGDSS
                     KMRVGDQVAAIGNPFGLSGSMTSGIVSQLGRLLSEPGASAFSIPDVIQTDAAVNPGNS
                     GGPLLNMRGEVIGINTAIQSGTGEFAGIGFAIPSKTVSKIVPILIKERQYKHPWLGIS
                     GIDIDPDLANILQLKDTRGFLIVSVATDSPAEKAGLHGASETKEIDGIRYQIGGDVIL
                     SVDGNDVRKIDDILIYLQHEKQVGDTINLEILREGRITNFELVLQERPNLMQQ"
BASE COUNT         1593 a          785 c          909 g         1336 t
ORIGIN      
        1 atggcaagat aatttgtctt ccctcatcta ctaaattttc catgcatgga ttgtttgaat
       61 caggtgtgag agaaaaattt cccaaagtat caaaaaattc ttgtggatat aggatagatc
      121 aggttacaaa caaatcagac tcgcagaaaa ttattgcagg ttcggaagga acactaggca
      181 tcatcttgtc tgcaaaatta aagacgtttc caattaaaag aaaaattaca ctttcaatac
      241 tagcgtacaa gacaataaat caggcagtgg cagatgttgt aaaaatcatt ggcttgaaac
      301 catctgcgct tgagattgta gaccacaaca tagcaaaaaa catcgatgta agactagcaa
      361 gaaatacaga gtgcttgcta tttgcagagt ttgacgataa tatcagaaaa aatacgtacc
      421 atctaaaaaa catcacacat ggaaagataa taaagacatt tgtaaaaaat catgaaataa
      481 aaaagtggtg ggattttaga aatactgcat tatcatacag tcttagaagt ataaatccaa
      541 aacaaagtat gcctaccata atagaggatg ctgttgttcc agttgagaat ctaggtttgt
      601 tggtggacat aatcaaaaaa atatcaaaaa aatacagact gaggtttgtt ctttacggac
      661 atgctggaaa tgggaatctg catgtaagac caatacttgg acataaaaac aaaaagatca
      721 tgaaaaaaat agctagcgag tattttgata aaataatagg aattggtgga agcataactg
      781 gtgaacatgg agatggattt gcaagatcag aatttgtgag gcaacaatat ggagatgatg
      841 tttattcagt ttttaagaaa ataaaaaaca gctttgatcc aaaaaacatt ctcaacccag
      901 gaaagataat tacacacaaa accactgttg tcaagaatct gagaatgtag ttattttatt
      961 tttgtaccaa gtggcacatc ctcgctcaca gttaaccaga aaggtctgtc atttacgtct
     1021 gctgccaaaa gcattgcgtt tgattctagt ccagcaattt ttcttggttc caaattagct
     1081 ataactacta ctgtttttcc aactaattct tctggttgat aatattctgc accgccaatt
     1141 atgacttcac gcttttcttc acccagatca ataacgcctt ttattatctt ggacttgcct
     1201 ggaatttttt ctgtagaaac aattttagct atcctaagat caagttttgc aaaatcatcg
     1261 tacgtgatgt ttgtcatagt gaagttaatt ttgtcgcatt aaaatttatt tcgaaactaa
     1321 ggaggatttt tactatatgg ttcaaatata aaagacattt acaatgtagt tatgtctaga
     1381 gatctatttt taaaaaaata atttaagcat ttttcagctt aaaatcttcg ttcgccacgg
     1441 tgttttggta aacactctcg gcaatatact ggtcttcctt cttttggtag gaatggtacc
     1501 tgtgtttctt ttttgcaatc tgcacaagtg gcagggtgca tttgtctgtc tgttgacatt
     1561 taatttctgg gaaatgttgc cacataagaa ctaaacacag atacgaaatc cacattttac
     1621 tcagatattc tagatcatct cgtgacttga agactaatat gtagattgta aaaaactgcc
     1681 catgttggct ataatcgagg catcagtatc catacgtgct ccagtagaca aggtctggaa
     1741 tatcatatct gatcttgaca atgagccaaa attttggaaa gggacgaaag aggtaaggaa
     1801 catatcaaaa gatggtaaca ccgttacgcg cgaagtcact attgctttca aggattccaa
     1861 atgtatgcaa actgtaacgt tgactccaaa agagagcatt catacaatat ttacaaaagg
     1921 aatcataaac ggtacaaaaa tagtcagtct tactgctcaa caaaatgaga ctttgctcaa
     1981 agcagtttgg gatatcaaac tttcaggaat gatgggcatg tttactggaa tggtaaaaaa
     2041 acacatccaa agtggtaccg aacaagcact tgaaggcata aaacaagaag cagagcgata
     2101 gtccatggaa tttgccttgg atgtattcaa ctatattcta tcagtaatca tgattggggt
     2161 ttcaataaca tggattcttt tgataagatc aatgtggagc tcatttagag attcaccatt
     2221 tttggacagg tttgatgcaa aaccgcattc aaatcctaaa gtttctgtga ttctaccagc
     2281 aagaaatgaa gaaaggttta ttgaaaaatg tcttaactcc ctgcttaacc aagattacca
     2341 gaattatgaa attatagtaa tagatgatcg ctcagaagac aataccggaa acattatcaa
     2401 aaaaatttct gaaaaaaatt ccaagataat tcatgtcact gcacaaccaa aaccagaaag
     2461 atggatagga aaaaactggg cgtgttttga gggtttcaaa aaatcgacag gggagctttt
     2521 acttttcaca gacgcagaca caaaacattc aagaagcatg atctcacttg cagtctcaca
     2581 tctgcacagc gaaagtcttg atgctttgac tgtgattcca aagatgctat gccttgacag
     2641 atggacaaaa ataactctac cagtgctttc cacgttttta cacacaagat tttctgcgtt
     2701 acgagtaaac gatccatcaa aaaagacagg atattttttt ggtagttttt tcattatcaa
     2761 aagagaaacc tatgaatctg ttggaaccca tgagggagta aaaggcgagc ttgtagaaga
     2821 cggtgcgtta ggaaaaaagg taaaagaatc aggattcaag atgaaaatgg tacgcggcga
     2881 acatctcctt gaagcaatct gggcacgcga ttttaccaca ttatggaacg gtctcaaaag
     2941 acttgtaata ccactttact tgcagagcaa aaagatagca gttggaatat tttttgcagt
     3001 attgtttttg cttttcatgc cgtttccgat tttagcatat tctagcatat ttgtaagtat
     3061 ggcacaatca ttttccgttc tttttttaat atccatagtc acaacatcgc tgctgtattt
     3121 tgctgcagtg cttgaggcaa aaaaaggatt aggactgagt acaattcatg ctctgtgtgg
     3181 gccagttgga agttttatta tcgtagcagg atttggaagt ggaattttgc aagctacaag
     3241 taatacatca gtcacgtgga gaggcagaac atattctatg gtagatcagg ttcaaaactc
     3301 aataagcgta tagtgagaat aaagtaaact tgaacattgt tcagtaagaa ttatagataa
     3361 acgtaaagca gatgattttg tggataagac aagcaccgtt tttggcggag tcataggcgg
     3421 aatcatagta gtaatagtgt tcgcactgta ttttgcacca gatggagtca gtgcgcctcc
     3481 aaaagacatt gttacttcaa atggacacga tttagaaacc gttggtgagg taacatcaat
     3541 tccaaaaaac tttgatgaga ttacacttgc agatcttttt gacaaatcag atgatggagt
     3601 agttaaagtc tccgttcgta aatcattaga aactccaaga agcagttcac tgggttcggg
     3661 atttgtctat gatgtaaacg gccacatcat aacaaataat catgtggtag atgatgcaca
     3721 aaaaatcaca gttacgtttc ttgatggaac ttcatacaat gcagatgtta ttgggactga
     3781 tccatttact gatctagcag taatcaaagt tgatgtcgac cctgcacttt tacacccatt
     3841 accaatagga gattcttcaa agatgcgtgt tggtgatcaa gttgcagcaa ttggaaatcc
     3901 ctttggactt tcaggttcca tgacttcggg catagtaagt cagcttggga gacttttatc
     3961 agaaccagga gcttcagcat tttccatacc agatgtcatt caaactgatg cagcagtaaa
     4021 tccaggaaac tctggaggcc ccttgctcaa catgagaggt gaagttatag gaataaacac
     4081 cgcaatccaa tccggcacag gtgagtttgc agggataggt tttgcaattc cttccaaaac
     4141 cgtatcaaag atagttccga tattaattaa agaaagacaa tacaagcacc catggcttgg
     4201 aatatctgga atagatattg atcctgatct tgccaacatt ttgcaattga aagatactag
     4261 aggatttttg atagtaagtg tagcgacaga cagcccagct gaaaaagcag gactacatgg
     4321 agcctctgaa acaaaagaga tagacggtat acgatatcaa atcggaggag atgtcatttt
     4381 atcagtagat ggtaatgatg tacgaaagat tgatgacatc ttgatttatt tacagcatga
     4441 aaagcaagtt ggcgatacta tcaacttgga aatactaaga gaaggacgca tcacaaactt
     4501 tgagttagtt cttcaagaaa gaccaaatct gatgcaacaa taatacaagc ataaatagtg
     4561 ccaacaaagg tatttgtggt gttgagcaag ctcatttcaa attatgaggc attaaacaag
     4621 gtt
//
LOCUS       DATIDL010000048         3002 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrosopumilaceae archaeon isolate SMAG_U16342
            k141_2884050, whole genome shotgun sequence.
ACCESSION   DATIDL010000048 DATIDL010000000
VERSION     DATIDL010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935836
            Sequence Read Archive: SRR25158326
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosopumilaceae archaeon (soil metagenome)
  ORGANISM  Nitrosopumilaceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrosopumilales;
            Nitrosopumilaceae.
REFERENCE   1  (bases 1 to 3002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/04/2024 05:09:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,520
            CDSs (total)                 :: 1,474
            Genes (coding)               :: 1,474
            CDSs (with protein)          :: 1,474
            Genes (RNA)                  :: 46
            rRNAs                        :: 1 (5S)
            complete rRNAs               :: 1 (5S)
            tRNAs                        :: 43
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3002
                     /organism="Nitrosopumilaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2884050"
                     /isolate="SMAG_U16342"
                     /isolation_source="soil"
                     /db_xref="taxon:2202732"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..367
                     /locus_tag="VLB45_02045"
     CDS             <1..367
                     /locus_tag="VLB45_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010195506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HSD04520.1"
                     /translation="TQILAYQLMGKESNGNTCPFLDTESNERSPHGGYKCRIYENRPL
                     ACRAYPVIETSPVVLDSKCKFCESCGNTAGNVNSELESLVKIKTRMDTTAPFIWRYAT
                     GIGDESNKDKIASGWFLEI"
     gene            complement(364..522)
                     /locus_tag="VLB45_02050"
     CDS             complement(364..522)
                     /locus_tag="VLB45_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007551286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04521.1"
                     /translation="METETEKCSVCNGSLEHKYYPMETWNIKGVLCGKCYSKKIAEFY
                     PGTHERIN"
     gene            complement(670..2424)
                     /locus_tag="VLB45_02055"
     CDS             complement(670..2424)
                     /locus_tag="VLB45_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007551285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIPR family protein"
                     /protein_id="HSD04522.1"
                     /translation="MAERSNGLLEYIPGARPVLLQSTNGQPLEGFSQNIRDSIREYAE
                     DAQNEVEKGHNFLQWVLTRVFEATEDDATDAIVDGANDLGIDAYLPVDFSEGTIRLFQ
                     SKYGTSHSMEAIAKFKEDVRNLLTRDVTKMRPELARLVTQIREKNLKIECVYVTDQKV
                     EYQNQDSFEVYDIDIIVQRLWDRIKKPAAGKKATIRLESKLKYHNTLLGILKLRELTN
                     FVTKNRDYVFESNIRQWMQFKTTVNKGIRETLQESPDKFFYYNNGITIVVSDFEEVED
                     NILTLHAPQIVNGAQTSNSILDHAKRTHNLEGSITVTIIKAADERDQNNITKYRNSQN
                     AVRGKDLVSLMDFHKSIKSQMENFGYFYEIQAGSFDSKSKSQQSEFNGDPTYNKYLPE
                     NHKKVIVAKDAIQVLVAGIEQRPTEAYSSPAQFLPRGSKYDDVFNEKLKDDCRLLLYP
                     YLVKEYAKKPLNYGKKGGHKTKRYATLFFVAVYFKILHEKILLTKGDFKEDISKLEPI
                     FKSVKLNQRIFKLTDTIVTKFLEDTIVDDEMDAANTYHNFFSHNIWQDSMIRVIEKKI
                     KQEEDELNSIKKIVQDLF"
     gene            complement(2489..2560)
                     /locus_tag="VLB45_02060"
     tRNA            complement(2489..2560)
                     /locus_tag="VLB45_02060"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2526..2528),aa:Cys,seq:gca)
     gene            complement(2602..2820)
                     /locus_tag="VLB45_02065"
     CDS             complement(2602..2820)
                     /locus_tag="VLB45_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histone"
                     /protein_id="HSD04523.1"
                     /translation="MTSEFGLSAMYRILKKSGAERVSDESADELRRILEEIGVAIAKS
                     AVDMSVHAGRKTVKAEDVRLAAKPFAKF"
BASE COUNT          835 a          566 c          528 g         1073 t
ORIGIN      
        1 aactcaaata cttgcatatc agcttatggg aaaggaatcc aatggaaata catgtccatt
       61 tttggacaca gaaagtaacg agagatctcc tcatggtgga tacaaatgca gaatctatga
      121 gaacagacct cttgcctgta gagcatatcc agtaattgag acatcgcctg ttgtcttgga
      181 ttcaaaatgt aagttctgtg aaagttgtgg aaacactgca ggaaacgtaa actctgaact
      241 ggaatcactt gtgaaaatca aaactaggat ggatacgact gcgccattta tttggcgata
      301 tgcaactgga attggtgacg aatcaaataa ggataaaatc gcatctggtt ggtttttaga
      361 aatctagttt attctttcgt gggttcctgg ataaaattct gcaatctttt tagaataaca
      421 tttgccacac aatacgcctt tgatattcca tgtttccatt ggatagtatt tgtgttctaa
      481 agatccgtta catacagaac atttttcagt ttctgtttcc aaaccaatcc agctccttta
      541 ttttcgttat aaaaatcatg ttaaatagta cctcttagtg gaataatgct gcggtagaat
      601 ggatggctaa tgcggacctc ctgcaagaga cctatcggtc acccatctgc cgctttttgt
      661 attaaaaatt tagaaaagat cttgaactat ttttttaatg ctgtttaact cgtcttcttc
      721 ttgttttatc ttcttttcaa taactcgtat cattgaatcc tgccaaatgt tgtgagaaaa
      781 gaagttgtga tatgtatttg cagcatccat ttcgtcatca actattgtat cttctaagaa
      841 ctttgtaact atggtatccg taagtttaaa aattcgctga ttcagcttga cacttttgaa
      901 aattggttca agttttgata tatcttcttt aaagtctcct ttcgttaaca aaattttttc
      961 atgtaagatc ttaaagtata ctgcaacaaa aaagagtgta gcataccttt ttgttttgtg
     1021 acctcctttc ttgccataat ttagcggttt tttagcatat tcttttacga gatagggata
     1081 cagaagtaat ctgcagtcat cttttagctt ctcattgaat acatcatcgt atttgctacc
     1141 tcgaggtaaa aattgcgcag gtgaactgta tgcctccgtg ggtctttgtt cgattccagc
     1201 cactagtacc tgtattgcgt cttttgcaac aattaccttt ttgtggtttt ctggaagata
     1261 tttgttgtat gtaggatctc cattgaattc actttgttgt gattttgatt tgctgtcaaa
     1321 agaaccagct tgaatctcat aaaaatatcc aaaattttcc atttgggatt tgattgattt
     1381 gtgaaagtcc attagagaaa caagatcttt tcctcttact gcattctgag aatttctata
     1441 ttttgtaata ttattctgat cacgttcgtc tgcagccttg attatggtta ctgtaatact
     1501 tccttcaagg ttgtgtgtac gctttgcatg atctaatatg gaattagaag tttgtgctcc
     1561 gtttactatc tgaggagcat gcagtgtcaa tatgttgtct tcaacttcct caaagtcact
     1621 tactactatg gtgattccat tgttgtagta aaagaatttg tcaggtgatt cttgaagtgt
     1681 ttctcgtatt cctttgttta ctgtagtttt aaattgcatc cattgtctga tatttgattc
     1741 aaagacataa tcacgatttt ttgtaacaaa gtttgttagt tctcttaatt ttaaaattcc
     1801 aagtaacgtg ttatgatact ttagtttgct ttctagtctg atcgttgcct tttttccagc
     1861 tgcaggtttt tttatcctat cccaaagtcg ttgtacgatg atatctatat cgtatacttc
     1921 aaatgaatcc tgattttgat attctacctt ttgatccgtc acatagacgc attctatttt
     1981 tagatttttt tcacgtatct gggttacaag gcgtgcaagt tcaggccgca tcttcgtaac
     2041 atcacgagta agcagatttc ttacatcttc tttgaatttg gcaattgctt ccatagaatg
     2101 tgacgtccca tattttgatt gaaaaagacg tatcgtaccc tcagaaaaat ctactggaag
     2161 atacgcatct atacctaaat cgtttgcacc gtcaactatg gcatctgtgg catcatcttc
     2221 agttgcttca aaaacacgtg tcagcaccca ttgcaaaaag ttgtgtccct tttctacttc
     2281 attttgagca tcttctgcat attctctaat gctatcccgt atgttttgtg agaaaccttc
     2341 cagtggttgt ccgtttgtag attgaagtaa tactggacga gctcctggaa tgtattctaa
     2401 tagaccgttg cttctttctg ccaaactgag atgattagaa aattatgtat ttagagaagc
     2461 tggttattgt attatctaat tactacaaag gtgaggaagg gattcgaacc cctatagatt
     2521 cgctttgcag gcgaacgcat agccgctctg ccacctcacc gcaatcaaaa aggtcgccaa
     2581 tgaacaatta aatcttttag attagaattt tgcaaatggt tttgcagcta acctgacgtc
     2641 ttctgccttg accgtctttc ttcctgcatg aaccgacatg tctactgcac tcttggctat
     2701 tgccacacca atttcttcaa gtattcttct taattcatct gcagactcat cactgactct
     2761 ttctgcacct gattttttta gaatgcgata cattgcagaa aggccaaatt cagatgtcat
     2821 gaaatggttt ttcttacccg tgataaaaga cttcaagtgg aaaaaaacct acgcaaggat
     2881 tgatttctac agactatttt aaggagaaaa ataaagacct gccatgagct ggttaactga
     2941 catccacatt catctctcag atccagagta ttctagtgat ctaccattta ttattaaaag
     3001 ca
//
LOCUS       DATIDL010000049        18100 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrosopumilaceae archaeon isolate SMAG_U16342
            k141_2481500, whole genome shotgun sequence.
ACCESSION   DATIDL010000049 DATIDL010000000
VERSION     DATIDL010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935836
            Sequence Read Archive: SRR25158326
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosopumilaceae archaeon (soil metagenome)
  ORGANISM  Nitrosopumilaceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrosopumilales;
            Nitrosopumilaceae.
REFERENCE   1  (bases 1 to 18100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/04/2024 05:09:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,520
            CDSs (total)                 :: 1,474
            Genes (coding)               :: 1,474
            CDSs (with protein)          :: 1,474
            Genes (RNA)                  :: 46
            rRNAs                        :: 1 (5S)
            complete rRNAs               :: 1 (5S)
            tRNAs                        :: 43
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18100
                     /organism="Nitrosopumilaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2481500"
                     /isolate="SMAG_U16342"
                     /isolation_source="soil"
                     /db_xref="taxon:2202732"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1015)
                     /locus_tag="VLB45_02070"
     CDS             complement(<1..1015)
                     /locus_tag="VLB45_02070"
                     /inference="COORDINATES: protein motif:HMM:NF013568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HSD04524.1"
                     /translation="MSQVLHPIEHKIIKTLKATPNLTFEELAKNAQLAIDQVRRGLEW
                     LKYKNLVEVRESEVFFASLGQNGKDAFEKGLPERRLVNYLTKHTVCTFEDAKAALLES
                     EFNAAISNARQNGWIEIAKGQTSNIISLKDDIKNSPEEELLTILVNRTPVSEIKNKNA
                     FESLKKRPNFIVLDSVKSTVANLTAQGTETAGKLSETVGERKITSEMLTSGKWKDVTL
                     APIDVEAAAPEVFAARTHPLQDVINEVREIFVSLGFAEILGTLTQSSFWNFDALFTPQ
                     DHPAREMQDTFYLKNISANKIATPSQISKVSESHKKNWKYDWKLSEAKRMVLRTHTTC
                     VTIK"
     gene            1068..2183
                     /locus_tag="VLB45_02075"
     CDS             1068..2183
                     /locus_tag="VLB45_02075"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HSD04525.1"
                     /translation="MSSDEFTVTPWSVEGDVDYDKLIQKFGTEKISPELLERIKKHTG
                     DLHQMLKLGYFFSHRDLDVVLSEYEKGNKFYLYTGRGPSGSIHMGHILPWIFTKYLQD
                     KFNAKLIFQITDDEKFLYSDEKSFEDVSKYTKENILDIIAVGFDPKKTKILIDSKDIK
                     QIYPISLEIAKRITYSTVKAVFGFQNSTNIGMIGFPPIQAAPCFLPSVIEGKPTPVLI
                     PAAIDQDPYWRVTRDIAEKIGYPKPAQIHSKFLPGLGMKGKMSSSIPETAIFTTDEDD
                     VIDKKISNSFTGGQPTIELQRKLGANFNICPVFWYLRYFFDSEKDSDERARKCKGGEL
                     LCGECKCDLKDATKPFITEFKKKREKAKDQIDKFLFE"
     gene            2188..2469
                     /locus_tag="VLB45_02080"
     CDS             2188..2469
                     /locus_tag="VLB45_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04526.1"
                     /translation="MKKKFVCDDEYEAQKLASLIYVKQDSETFITGVLEIVENEVIVS
                     LKDKSAHSILLKDRLEVERFADFIQSVVEKKHKITNAEISKNTVEITKD"
     gene            complement(2466..2798)
                     /locus_tag="VLB45_02085"
     CDS             complement(2466..2798)
                     /locus_tag="VLB45_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016940196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04527.1"
                     /translation="MKGITNHMLNEMSENVVNLKEGLNPENIAYWYKKVIDEAKDMAP
                     SWLQDKIKVKQDPILHMKFNLDISKRAVRYFMMAIENNIDEMPYSTRLYFLKVQEILT
                     HEMDKTLV"
     gene            complement(2864..3328)
                     /locus_tag="VLB45_02090"
     CDS             complement(2864..3328)
                     /locus_tag="VLB45_02090"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010194923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HSD04528.1"
                     /translation="MSKFKAVALGGTFDVIHKGHLALLQKGFSISSKVIIGLTGDELA
                     VKKGKKLLHNYSQRLEALKSVIEKNFPNSHYEISRLDNDFGPAVLESEVDALVVSEET
                     IEQGRVLNKLRHERHLSPVEVVTVPMVLAKDGKRISTTRIKNSEIDPEGNLR"
     gene            complement(3354..4433)
                     /locus_tag="VLB45_02095"
     CDS             complement(3354..4433)
                     /locus_tag="VLB45_02095"
                     /inference="COORDINATES: protein motif:HMM:NF038353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxLYD domain-containing protein"
                     /protein_id="HSD04529.1"
                     /translation="MKRILIAILLVFFILPTSAWAQYNQPSATLTVEVLSPYSYKADD
                     GSTVVLGEVENKNNFPVTGVKIGVSFYDETGKMTEYKTGTTLIKVIPAKGKAPFSISS
                     TKPGEITDISVNLTGFNSSPARPQVLNLSPDILEISEDLTLSGTIINGGTIPTTGTTI
                     HLISYDAFGRVVGIASTTPDPEDVDVGKTSVFSITTAPKEIARSYKIVAESDNYQSTT
                     VDVKNVKTTISSLTKLVTINDVSVTDLIGNKYSTIPVGSTVKIASEIWIKYADEQSEQ
                     PYVYYVQIKQIETEKLAQDEDVVAPVEFIGIVPGVFHGIEKQDVSVNWIPDKQGAFFV
                     EVYVWDQNAVALASPSSMINVMLVK"
     gene            complement(4754..4825)
                     /locus_tag="VLB45_02100"
     tRNA            complement(4754..4825)
                     /locus_tag="VLB45_02100"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4791..4793),aa:Ala,seq:tgc)
     gene            4912..5472
                     /locus_tag="VLB45_02105"
     CDS             4912..5472
                     /locus_tag="VLB45_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HSD04530.1"
                     /translation="MVLLKSELVLKTGDKAPDFDLLGTDDKKHSLSEFKVYKGLLVVF
                     MCNHCPYVQAKTKALVEIYDKFKDKVAIIGINSNDPTNYPDDSFENMKRFAKEKGIKF
                     TYLVDETQQVAKRYGAVCTPDPFLFDQDKKLVFHGRIDNAMSPEDTATEKTMILNIEK
                     LLQGKKIEKDFDPSMGCSIKWKSQQT"
     gene            5498..5585
                     /locus_tag="VLB45_02110"
     tRNA            join(5498..5536,5550..5585)
                     /locus_tag="VLB45_02110"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5533..5535,aa:Ile,seq:gat)
     gene            5640..6425
                     /locus_tag="VLB45_02115"
     CDS             5640..6425
                     /locus_tag="VLB45_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010195207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HSD04531.1"
                     /translation="MEEKFVKVGTNKIRYLQEGKGKNVVLVHGLGASAERWAYVIPRL
                     SKKYHVIVPDLIGFGYSDKPSIDYTPELFSSFIFDFLNTLDISKASMVGSSLGGQIVA
                     ECAITQNKMIEKIVLVSPSGLMRHSTPTLDAYMMAALYPNPDGAKSAFQMMAGPHRDI
                     DAKTINGFVERMTLPNAKMAFMSTVLGLKNSVSMEDRLAKISVPTMIIWGKEDSLIPI
                     KYSNNFLSAIKNCQFVEMDGCGHTPYVEEPEKFSEILLKFLSQ"
     gene            6487..6900
                     /locus_tag="VLB45_02120"
     CDS             6487..6900
                     /locus_tag="VLB45_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HSD04532.1"
                     /translation="MNGNNNPYDPTTMFKEWIQKSGKAQQEFMKTFGSMMGQQKQEFD
                     PVETLKDIATKGAKVQSEAMENFGNIQSKAIDSAFKLAQSMPQFVSWGAYKTSVGSNG
                     RISIPEAEREALGLKEGDLVQVVIIPIEKKSKKVR"
     gene            6905..7324
                     /locus_tag="VLB45_02125"
     CDS             6905..7324
                     /locus_tag="VLB45_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007550789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04533.1"
                     /translation="MSKTTPETKEIFAVYKQNVEKYFTEVEKTAPQYLQSITNLQQEY
                     TAAWKNAIESAISLQQEFATKAGINTNVPAAVVKLVNDATEELIKAKAVQNKLVLASI
                     DAVQQNVKTFNDNAKTFATLNENIVQSWISACTPTRN"
     gene            complement(7347..8417)
                     /gene="phaC"
                     /locus_tag="VLB45_02130"
     CDS             complement(7347..8417)
                     /gene="phaC"
                     /locus_tag="VLB45_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class III poly(R)-hydroxyalkanoic acid synthase
                     subunit PhaC"
                     /protein_id="HSD04534.1"
                     /translation="MENEIKIDPALVAEFLKFNKNVMDAPKMVPAPNEINLEMTPYDV
                     AYAEDKVRLLHFNSPVQKQNQIPLVIAYALINRYHILDIQPHKSWVKNLLNQGFDIYM
                     IDWGTPSHIDKYLSFDDYVNGYIENCVDFVRSETSVDKVSLQGYCTGGTLATVYTALH
                     PDKVKNLIATAPVIDGKRDTTVVGNLAKHVDVDKMVETIGNMPPEFMYYVFSVLKPFE
                     QGIEKYINFFKNIEDKNYVDSFLRVEKWLSETPPIPGELFKQWIKDIYQDNLLIQDKM
                     YVGGKLVSLKNIKMPIFTQVAVGDHLVSPECSMPLHYAVGSEDKTLRIYPTGHVGMIA
                     SSFSQKNVLPELGQWLKERSVK"
     gene            complement(8430..8984)
                     /locus_tag="VLB45_02135"
     CDS             complement(8430..8984)
                     /locus_tag="VLB45_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008299363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly(R)-hydroxyalkanoic acid synthase subunit
                     PhaE"
                     /protein_id="HSD04535.1"
                     /translation="MVSEPEYQKTLDYYKHLSLFWTDIVYLMASKPAALTSAGPMRKF
                     AMQSKKLATEMIESNEDLIEFNTRLAEYYKQLSDTWIEAQKKFNKKIPEIPNDAETLE
                     ASKRVWIDMFENDFTQLFDSEKFGENFGKLISSELELSKHWNNMLSILLETANVPTKH
                     DLDEVYKELHSLRKRISKLEKNGK"
     gene            9092..9442
                     /locus_tag="VLB45_02140"
     CDS             9092..9442
                     /locus_tag="VLB45_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6659 family protein"
                     /protein_id="HSD04536.1"
                     /translation="MDYETICKNVMDLDSKIRFAGMINNKGRLIAGGMKEGKKPFEDT
                     KDDEMLFMELVLRTKMRHEFDSQLGRVKFAMSYREKAVVMSFPAGDNVMLVSAEKEID
                     FAKIPFKILKILEN"
     gene            complement(9439..10335)
                     /locus_tag="VLB45_02145"
     CDS             complement(9439..10335)
                     /locus_tag="VLB45_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing protein"
                     /protein_id="HSD04537.1"
                     /translation="MARGYQITKIKEKLVDVLQNSKTGLSGIEIAEKLGISRITIAKY
                     LNVFAAEGLIRQRNIGNVNLWIVEEGVDQLRFPADFFQVKNKYLQYLLTASHKEAHNI
                     LRNSLHSGADPIKITTEVIIPAIQSVQDLYEKGKIGKSEKNFLDEIILDSIQIIRLIE
                     TESDPKKNVIVFSADFRDSLLSKAASAALHAGGWKVSSLGDMSSAIDVMFDIDLQKFL
                     NKFWTKKQGIMIILVFSATENGLKFFAEAVNSSKAKFGKTLHLALCTNLSKKTKVKAD
                     FISDDLEDALQWCQTVYESSQS"
     gene            10718..11104
                     /locus_tag="VLB45_02150"
     CDS             10718..11104
                     /locus_tag="VLB45_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04538.1"
                     /translation="MPKAGFKSITVSETVYNKFHDIYKKNRQELAMKGINSFAGYITF
                     MMEDLMQKDETFARYAPKLEKIAVDDNRVVIKDNIKNRIAEVAIQNGELYCMLCEEKD
                     CFHVGFVFSLPDVYEVLNARGVKHQR"
     gene            complement(11111..11230)
                     /locus_tag="VLB45_02155"
     tRNA            complement(join(11111..11155,11173..11186,11205..11230))
                     /locus_tag="VLB45_02155"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11174..11176),aa:Leu,seq:taa)
     gene            11330..11403
                     /locus_tag="VLB45_02160"
     tRNA            11330..11403
                     /locus_tag="VLB45_02160"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11364..11366,aa:Thr,seq:ggt)
     gene            complement(11406..12269)
                     /locus_tag="VLB45_02165"
     CDS             complement(11406..12269)
                     /locus_tag="VLB45_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007551336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HSD04539.1"
                     /translation="MKQQYKQILSLAPKPFVKWAGGKRQLIPILSQYMPKKFDTYFEP
                     FLGGGAVLFHLISENPKLECFVSDLNSNLVLSYVTIRDRVEELVSALEAHSDNYFKNP
                     KEYYYEVRESNPKSQIEKVSRLIFLNKTCFNGLYRVNSKGKFNVPLGKYENPNIVNKE
                     NLVAVSHALHSKDISIKCQDFPKALENAQKNDFVYLDPPYQPVSQTSNFTSYTNDSFG
                     YEDQERLFLEFQRLDSKGCKVMLSNSKSDEVLNLFEDYSDKIIEISANRFINSDSKKR
                     TGHSELLIKNY"
     gene            complement(12416..13066)
                     /locus_tag="VLB45_02170"
     CDS             complement(12416..13066)
                     /locus_tag="VLB45_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia monooxygenase"
                     /protein_id="HSD04540.1"
                     /translation="MVWLRRCTHYLFIVVVAVNSTLLTINAGDYIFYTDWAWTSFVVF
                     SVSQTLMLVVGAAYYLTFTGVPGTATYYALIMTVYTWVAKGAWFALGYPYDFIVTPVW
                     IPSAMLLDLVYWATKKNKHSLILMGGVLCGMSLPLFNMINLILVADPLETAFKYPRPT
                     LPPYMTPIEPQVGKFYNSPVALGAGAGAVLAPTMAALGCKLTTWTYRWMAAWSKWD"
     gene            complement(13183..13539)
                     /locus_tag="VLB45_02175"
     CDS             complement(13183..13539)
                     /locus_tag="VLB45_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007551338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04541.1"
                     /translation="MTLPKGFGSGGGGSSSDVNKMIGRRVENMMGLYTASYLAAWLST
                     FAGTAVGYFYYPWAYPAPSGHYAFIVLTIIEAIGYLFCVKVGEEGSSKNSNGILAGVL
                     GATAVATVLIVTYIHG"
     gene            complement(13655..14218)
                     /locus_tag="VLB45_02180"
     CDS             complement(13655..14218)
                     /locus_tag="VLB45_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methane monooxygenase/ammonia monooxygenase
                     subunit C"
                     /protein_id="HSD04542.1"
                     /translation="MAQMPALIPKEVEIQRLKKIWMIVISMGSTAASVEVDNFVDGSL
                     HQTSIRDSAFTPAHWWLYSHFVALPLGWGAVSIYDRKVPVLRGPNNSMNTGLKMTILG
                     YLATMFTIGVNEMWHFWYVEEIFAVPNHWMFNMGVVVAFMGALAYVVRVYARLVELGA
                     ETPGENPYVAEMYKMALEGKLYSRSIP"
     gene            14432..15055
                     /locus_tag="VLB45_02185"
     CDS             14432..15055
                     /locus_tag="VLB45_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methane monooxygenase/ammonia monooxygenase
                     subunit B"
                     /protein_id="HSD04543.1"
                     /translation="MRAKYISLVFLLWNSYGITMMNKKLIVAALGVIVALGTVGPSVG
                     QLLQQAEAHGVQAQLQSRFIRIEDETFSRQSLNTGETLTVKGTLVSLVQRDLRAWLSV
                     YGESTNAGNRWEFISRDPPGNVFDIPGNGVVDYTLVVKALEAGVYHMHTQLNIASIGP
                     GLGPGQTVVVEGENIVKPIPYANVAYQSIIIAVGYVVTFATRPWQVI"
     gene            15164..15685
                     /locus_tag="VLB45_02190"
     CDS             15164..15685
                     /locus_tag="VLB45_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04544.1"
                     /translation="MIWPGMGDPAKRKQTIKFLIITAAVAAGAYFASFAVTGSLSAND
                     PLKQCINNRDINYKISATFELIVDGIKVEIPANVGNSDECKKSLYTISNDGVIYAEWD
                     EKYDFEIGHFLWIYKFPLREMDESKSKIFVNDIQSPDFVHAKLENGYHYRGEFVSKAA
                     DTSKEHDFLPPES"
     gene            complement(15742..15990)
                     /locus_tag="VLB45_02195"
     CDS             complement(15742..15990)
                     /locus_tag="VLB45_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04545.1"
                     /translation="MLVGILSNFAGQIGPAYDPLFYDVMVYIFGTIMFAVFLLGVIAF
                     WMRVHGLGGDTRERWVSELDKHFEKEGIGLIPDNGKYW"
     gene            complement(16114..16422)
                     /locus_tag="VLB45_02200"
     CDS             complement(16114..16422)
                     /locus_tag="VLB45_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04546.1"
                     /translation="MNKPILIGATVLVLFVVSSLLPMAAAQYVGVGGGGQTTLEEQLE
                     LAKAKVQKAADNPATGSGTPYLDANGVVGGSVIVGAVFGGIAIAFVLKGRSGRFAKSN
                     "
     gene            16497..16988
                     /locus_tag="VLB45_02205"
     CDS             16497..16988
                     /locus_tag="VLB45_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008296762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HSD04547.1"
                     /translation="MKVTFMFCPKTMRPMGRVHSHRHGQSHSIRPVTPSSPTWVKQSP
                     QEIEELVLKYAKDGLTTSEIGVKLRDQHAIPLTKVITKKSVTEILEQKGTKPEMPEDL
                     NNIVRKALGLQKHLKTHTSDRRNVRSLELLEAKVHRLSSYYKKTGRIPKTWKYKSVIA
                     QLE"
     gene            16988..>18100
                     /locus_tag="VLB45_02210"
     CDS             16988..>18100
                     /locus_tag="VLB45_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HSD04548.1"
                     /translation="MAKLDGALSYFHDRVSESIKSGKNISVITHLDCDGITSGSITTK
                     ALIRSGARCTVRTVNEFSRNLIEKLKKDSRDLYIITDLGGGFAKDLDEILEDNWIVVD
                     HHQIPNEEMDNERVINAWKYGIDGGKEVSAGGMSYLVSKALHRENTDLAWIAVVSALG
                     DRQDQGEKKSFTGINLEIASTAKKNDQVEIDLDILLVGRETRPLPDALAFTSHPFIEG
                     LTWNRDACLSLLNSSGIKLKDGSRWRVPAELNEDEKRNLLQTISKFTSGKNATEIMDE
                     LVGYTYTLSGEDKRSFLRDAREFSTMLNSCGRIRKAGVGVAICMGDRTKMLEEGESIL
                     LEYRTVLRTYMNTLASERWRVVDNGQYLMVNGEGLVS"
BASE COUNT         5773 a         3334 c         3442 g         5551 t
ORIGIN      
        1 gctttattgt aacacaggtt gtgtgtgtac gcaagaccat tctttttgct tctgaaagtt
       61 tccagtcata cttccagttt tttttgtgtg attctgacac ttttgatatc tgagatggag
      121 tggcaatctt gtttgcacta atattcttaa gataaaacgt atcttgcatt tcgcgagcag
      181 gatgatcttg cggtgtaaat aatgcatcaa aattccaaaa gcttgattgc gttagagttc
      241 caagaatttc tgcaaatcca agactaacaa aaatttctct aacttcattt atcacatctt
      301 gtaatggatg tgtccttgca gcaaagactt cgggagctgc tgcctctaca tcaattggtg
      361 caagcgtaac gtctttccat tttccgcttg taagcatttc agatgtaatc tttctttctc
      421 caactgtttc agagagtttt cctgcagttt ctgttccttg agcagtaagg tttgctacgg
      481 tagattttac tgaatcaaga acaataaaat tcggtctttt tttcagagat tcaaaagcat
      541 ttttattttt tatttctgag actggagttc gattaactag aatggtaagc aattcttctt
      601 ctggtgaatt ttttatgtcg tccttaagcg agatgatgtt tgaggtttgc ccttttgcga
      661 tctcaatcca tccattttgt cttgcgtttg atattgcagc attaaattcg gattctaaaa
      721 gtgctgcttt agcgtcctca aatgtacaga ctgtgtgttt tgtcagataa tttacgagtc
      781 gcctctctgg cagacccttc tcaaatgcgt cttttccatt ttgtccaaga ctagcaaaaa
      841 atacttcaga ctcacgaact tctacgagat ttttgtactt gagccattct aatcctcgtc
      901 ttacttgatc aattgcaagc tgtgcatttt ttgcaagttc ttcaaaagtc agatttggtg
      961 tggcttttag agttttgatt atcttatgtt cgattggatg aagaacttgt gacaatagta
     1021 aaaagtgcag aaaagacttt ttaaacctta acctatctga atgatgaatg tccagcgacg
     1081 aattcactgt gactccatgg agtgttgaag gtgacgttga ctatgacaag cttatccaaa
     1141 aattcggaac tgaaaaaatt tctccagagc tgcttgagag aataaaaaaa catactggcg
     1201 acctgcatca aatgctaaaa ctcggatatt tttttagtca cagagatttg gatgtggttt
     1261 tatctgaata cgagaaaggg aacaaattct atctttacac aggcagaggt ccatctggtt
     1321 caatacatat gggacacatt cttccatgga tttttacaaa atacctacaa gacaagttta
     1381 atgcaaaatt gatctttcag attacagacg atgaaaaatt cctttatagt gatgaaaaat
     1441 catttgagga tgtttcaaaa tacacaaaag aaaatatctt ggacatcatt gcagtcggat
     1501 ttgatccaaa aaagacaaaa attctaatag attcaaaaga cattaagcag atttatccta
     1561 tttctcttga gattgcaaag agaataacat attctacagt aaaagctgtt tttggttttc
     1621 aaaactccac aaacatagga atgattggat ttcctccaat tcaggcagca ccgtgttttc
     1681 ttccttccgt gattgaaggc aaacctaccc ctgttctcat accagcagcc atagatcaag
     1741 acccctattg gagggtaaca agagacattg cagaaaaaat tggatatcca aaacccgctc
     1801 agattcactc caagtttctg ccagggttag gtatgaaggg aaaaatgtct tcttcaattc
     1861 cagaaactgc aattttcact actgatgaag atgatgtgat tgacaagaaa atttcaaatt
     1921 catttacagg tggtcaacct actattgaat tacaacgtaa acttggtgcc aactttaaca
     1981 tctgtcccgt gttttggtat ttgcgttact tttttgattc tgaaaaagat tctgatgaaa
     2041 gagcaagaaa gtgcaaaggt ggggaacttt tgtgcggcga atgcaaatgt gatctaaagg
     2101 atgctacaaa acctttcata acagaattta agaaaaagag agaaaaggca aaggaccaga
     2161 tagacaaatt tttgtttgaa taaagacatg aagaaaaagt ttgtttgtga tgatgaatat
     2221 gaagcacaaa aacttgctag tctgatttac gtcaaacaag atagcgaaac tttcatcacc
     2281 ggtgttttag aaattgttga aaacgaagta attgtatcgt taaaggacaa atcagcacac
     2341 agtattttgc taaaagacag acttgaagta gaacgttttg cagattttat tcagtcagta
     2401 gttgagaaaa aacacaagat aacaaatgca gaaatttcta aaaacacagt agagatcacc
     2461 aaggattaga ccagtgtctt atccatttca tgtgtcaata tttcttgaac tttgagaaag
     2521 taaagcctcg tagaataggg catttcgtct atgttatttt ctattgccat cataaaatat
     2581 ctcactgcac gctttgatat gtccagatta aacttcatgt gcagtatagg atcttgcttg
     2641 actttgatct tgtcttgaag ccatgaaggg gccatatctt ttgcttcatc tatgaccttt
     2701 ttgtaccagt atgcaatgtt ttctggatta agaccttctt ttaggttgac tacattctca
     2761 gacatttcat ttaacatgtg gtttgtgatg cccttcaaca gtgaatgcta gaaatttttt
     2821 gttttatttg acttactcaa agatagacaa gttgtttgtc aattcagcgt aaatttcctt
     2881 ctggatcaat ttctgaattt tttattctgg tagtagagat ccgtttgcca tcttttgcca
     2941 ataccattgg aacagtgaca acttccactg gagaaagatg tctttcgtgt cgcaatttgt
     3001 tcagcactct tccttgctct atggtttctt cacttacaac caaggcatct acctcgcttt
     3061 caagtacagc aggtccaaaa tcattatcaa gtctgcttat ttcatagtgc gagtttggaa
     3121 aatttttttc tatgacagac tttagtgctt ccagtctttg tgagtaatta tgcaaaagtt
     3181 ttttgccttt tttcactgcg agctcgtcac ctgtaagacc aataatcact ttggacgaga
     3241 tagaaaaccc cttttgcaat aatgcaagat gccctttgtg tatcacatca aaggttcctc
     3301 caagtgctac tgctttgaat ttggacatac agatactttg ttttctttta gatttacttt
     3361 actagcatca catttatcat tgaacttggg ctagccaagg ccactgcatt ttgatcccac
     3421 acatacactt ctacaaaaaa tgcgccttgt ttatctggaa tccagtttac tgaaacatcc
     3481 tgtttttcta ttccatgaaa tacgcctgga actattccta taaattcaac gggagctaca
     3541 acgtcttcat cttgggcaag tttttcagtt tctatttgtt ttatctgcac atagtaaaca
     3601 tagggttgct cagactgttc atctgcatat tttatccata tttcactagc tattttgacc
     3661 gtactgccga cagggattgt agagtacttg tttccaatta gatcagtaac tgatacatca
     3721 tttattgtaa caagttttgt aagtgagcta attgtggttt ttacattttt aacatcaaca
     3781 gttgtagact ggtaattatc cgactctgca acaatcttgt atgatcttgc aatttctttt
     3841 ggagcagtgg ttatgctaaa cacggatgtt ttcccaacat ctacatcttc tggatcgggt
     3901 gttgttgatg caattccaac aactcttcca aatgcatcat atgaaataag atggattgtt
     3961 gttccagttg taggtattgt tccaccgttt attatggtcc ctgaaagcgt aagatcttca
     4021 gatatttcca aaatatctgg tgatagattt agtacctgtg gtcttgcagg cgaggaattg
     4081 aatccagtca gatttactga gatatcagtt atttctcctg gttttgtaga actaatagaa
     4141 aatggagcct ttcctttagc aggaattact ttgatcagtg ttgtgccagt tttgtattct
     4201 gtcatttttc cagtttcatc ataaaatgaa acacctatct taacacctgt aactggaaag
     4261 ttattcttgt tttccacctc tccaagtacc acagttgagc catcatctgc cttatagcta
     4321 tatggagaaa gtacctcaac agtcaaagta gcacttggtt gattatactg cgcccaagca
     4381 ctagttggaa gtatgaaaaa tacaagtaaa atggcaataa ggattctttt catacactag
     4441 tacttttcaa aagtgattaa tcatgtacag tatatcataa tctaaacaac gtgcactaga
     4501 aggttttact aaactatcgt tagatgacca aataataaaa agatgtcaag atgttaaaac
     4561 atctatcttt tgcttttacg agcagtaatt gcacaccagt atactagacc tggagccaat
     4621 cctactatca aaggaatcca aactgctatt tcttctgatg gcatttaatt tccctataca
     4681 gcagagaata aattgatatt taaatccatc tttcatggac acgatcactt tagaggcgtt
     4741 atttaactaa agatggactg gtggggattt gaaccccaga ccccccgcgt gcaaggcggg
     4801 tattctaccg ctgaactacc agcccatcaa agggaattca agtcgtgtag attttaattg
     4861 ttgcctttgt ttgggcaaag attttattga catgtttcaa acaatttttt catggtactt
     4921 ctaaaatctg aacttgtctt aaaaacaggt gacaaggcac cggattttga tcttttaggg
     4981 acagatgaca aaaaacactc actttctgaa tttaaggtgt acaaaggctt gcttgtggtt
     5041 ttcatgtgta accactgtcc atacgtacaa gcaaaaacaa aggcactagt tgagatttat
     5101 gacaaattca aagataaagt tgctataata ggaataaaca gtaacgatcc tacaaactat
     5161 ccagacgata gttttgagaa tatgaagcga tttgcaaaag aaaagggcat caaatttacg
     5221 tatcttgtag acgaaacaca gcaggttgca aaaaggtatg gagctgtctg tacgccagat
     5281 ccttttctct ttgaccagga taaaaaacta gtattccacg gcagaataga caatgctatg
     5341 agtccagagg atacagcaac agaaaaaacg atgattctta acatagagaa acttttgcaa
     5401 ggaaagaaaa tcgagaaaga ttttgatcca tccatgggct gttccatcaa atggaaaagc
     5461 cagcaaactt aaatgaccgt cgggttggaa aattttcggg ccgatagctc agcttggctg
     5521 gagcgttcga ctgataaact tattcagata tcgaaaggtc gtgggttcga atcccattcg
     5581 gcccattttt ttaatggaaa agatttatct tattcagaat ccacggacga acagtactga
     5641 tggaagagaa attcgttaag gttggcacaa acaagatcag atatcttcag gaaggaaagg
     5701 ggaagaatgt cgttcttgta catggccttg gtgcgtcagc agaaagatgg gcatatgtaa
     5761 ttccccgtct aagcaaaaaa taccatgtaa tcgtgcctga tctaatagga tttggctata
     5821 gtgataaacc ttcaatagat tacaccccag aactcttttc aagtttcatc tttgatttct
     5881 tgaatacttt agacattagt aaagcaagca tggttggttc ttcactagga ggccaaattg
     5941 tagcagagtg tgcaataaca caaaacaaaa tgatcgaaaa gatagtttta gtatctcctt
     6001 caggactcat gaggcactcc acacctactt tagatgcata tatgatggca gcactctatc
     6061 caaatcccga cggtgcaaag tcagcctttc aaatgatggc aggacctcat agagatattg
     6121 acgccaagac cataaacgga tttgttgaaa ggatgactct acccaatgca aagatggcct
     6181 tcatgtctac agttcttggc ctgaaaaatt cagtctcaat ggaggatagg ctagcaaaaa
     6241 tttctgtacc aaccatgata atatggggaa aggaggattc actcattcct atcaaatatt
     6301 ccaataactt tctatcagca atcaagaatt gccagtttgt agagatggat ggttgcggac
     6361 atacaccata tgttgaagag cctgaaaagt tctcagaaat tctgctcaaa tttttaagcc
     6421 agtgaatggt tttatttacc aacaattacc aataggcata tatactggta tagacaatga
     6481 aaacctatga atggtaacaa taacccttat gatcctacaa caatgttcaa agaatggata
     6541 caaaaaagcg gcaaggcaca acaggagttt atgaaaactt ttggttcaat gatgggtcag
     6601 caaaaacagg agttcgatcc tgtagagacc ctaaaggata ttgcaaccaa gggagcaaaa
     6661 gttcaatcag aagcaatgga gaactttgga aacattcaat caaaagcaat tgacagcgcc
     6721 tttaagcttg cacaatccat gccacagttt gtttcgtggg gagcatacaa aacatcagtt
     6781 ggcagcaatg gcagaatttc gattcccgaa gcagaaagag aagcacttgg actaaaagaa
     6841 ggagatcttg ttcaagtggt aatcattcct atcgagaaaa aatcaaaaaa agtgaggtga
     6901 aaaaatgagt aagacaacac cagaaacaaa agagatcttt gcagtgtaca aacaaaacgt
     6961 agaaaaatac tttactgaag tagagaaaac tgcaccgcaa tacctacaat caataacaaa
     7021 ccttcagcaa gaatacaccg cagcatggaa aaatgctata gaatcagcaa tttctttgca
     7081 gcaagaattc gctacaaaag ctggcatcaa cacaaacgta ccagcagcag tagtaaaact
     7141 agtaaacgat gcaactgaag aattgatcaa ggcaaaagca gtacaaaata aattagtact
     7201 tgcctcaata gatgcagtac aacagaatgt caaaacattc aacgataatg caaaaacatt
     7261 tgctacgtta aatgagaaca ttgtacaatc ttggatctca gcatgtacac ctacacgaaa
     7321 ttagtgtaag ggtatttttt ccttttttat tttacagatc tttcttttaa ccactgacca
     7381 agctctggaa gaacattttt ttgtgaaaat gagcttgcaa tcattcctac atgtccagta
     7441 ggataaattc taagagtttt atcttcacta ccaactgcat agtgtaatgg catactacac
     7501 tccggagata ccagatgatc tccaactgca acttgagtaa atattggcat tttaatattt
     7561 ttaagcgata caagttttcc tccaacatac attttgtctt gtattaacaa attatcttgg
     7621 tagatgtctt ttatccattg tttgaaaagt tcaccaggga ttggaggggt ttcacttaac
     7681 catttttcga cacgtaaaaa gctgtcaaca taatttttat cttcaatgtt tttgaaaaag
     7741 ttgatgtatt tttctattcc ttgttcaaaa ggtttcaaaa cagaaaaaac atagtacatg
     7801 aattctggag gcatgttgcc aatggtctcc accatcttgt ccacgtcgac atgtttggca
     7861 aggttaccaa ccacggttgt gtcacgttta ccatcaatta caggcgcagt ggcaatcaga
     7921 ttcttgactt tgtctggatg tagtgctgta tagactgtgg ctagagttcc gcctgtacag
     7981 tacccttgta aagacacttt atccacggat gtttcactgc gtacgaaatc cacacagttt
     8041 tctatgtaac cattcacgta atcatcaaag ctgagatatt tgtcaatatg tgatggcgtt
     8101 ccccaatcta tcatgtaaat atcaaatccc tgatttagta gatttttcac ccaactcttg
     8161 tgtggttgta tatccaaaat gtggtatcgg tttatcaatg cataagctat gacaagtgga
     8221 atttggtttt gtttttgcac tggagaatta aagtgtagta aacgaacctt gtcttcagca
     8281 tatgcaacat cataaggcgt catttcaaga ttaatttcat ttggagctgg taccattttt
     8341 ggagcatcca ttacgttttt attaaatttc aaaaattcgg caacaagtgc aggatcaatc
     8401 tttatttcat tttccatctt ttacaccaat tatttcccat ttttttccag tttgcttatt
     8461 cgtttgcgca aagaatgtaa ttccttgtaa acttcatcaa gatcatgttt ggttggcaca
     8521 ttagcagttt caagtaaaat tgaaagcatg ttattccaat gttttgaaag ttcaagttcg
     8581 ctggatatca gtttgccaaa attttctcca aatttttcag aatcaaatag ttgtgtaaaa
     8641 tcattttcaa acatgtctat ccagacacgt tttgatgctt caagagtttc cgcgtcgttt
     8701 ggaatttctg gaattttttt attaaatttc ttttgtgctt caatccatgt atctgaaagt
     8761 tgtttatagt attcagcgag tcttgtgtta aattctataa gatcttcgtt ggactctatc
     8821 atttctgtag caagtttttt tgattgcata gcaaattttc tcataggtcc tgcagatgtt
     8881 aatgctgcag gtttacttgc cataagatat actatatcag tccaaaataa cgaaaggtgt
     8941 ttgtaataat ctaaagtttt ttgatattcg ggttctgaaa ccataaatag ataataaaaa
     9001 agatcgcaat aaatagatcg ccaattatac catattgcat tgacatcaaa ttcaacgaga
     9061 cagaaaacag gatgttatag gatgagacaa tatggattat gagacaattt gcaaaaatgt
     9121 catggatttg gactctaaga tacgatttgc aggtatgata aacaacaaag gcaggctaat
     9181 tgcagggggt atgaaagaag gcaagaaacc ctttgaagat acaaaggatg atgaaatgtt
     9241 attcatggag cttgttttga gaacaaagat gcgacatgag tttgatagtc agcttggacg
     9301 agtaaaattt gcaatgtcat atagggaaaa agctgttgtt atgagctttc cagctggaga
     9361 caacgttatg ttggtttctg ctgaaaaaga gattgatttt gcaaaaattc ctttcaagat
     9421 attaaaaatt ctagaaaact aagattgcga gctttcgtaa acagtttgac accattgtaa
     9481 agcatcttct agatcatcag agataaaatc agctttgact ttggtctttt ttgaaagatt
     9541 tgtacataat gctagatgta aggtcttgcc aaattttgct ttacttgaat taacagcctc
     9601 agcaaaaaac ttgaggccat tttctgttgc tgaaaacact agaattatca tgattccttg
     9661 ttttttggtc caaaatttgt ttagaaattt ttgtaaatca atatcaaaca tcacgtctat
     9721 tgcggatgac atgtcgccaa gagaagacac tttccagcct ccagcatgta atgctgcaga
     9781 agctgccttt gacaatagtg aatccctaaa gtcggcagaa aagacaatta catttttttt
     9841 tggatcactt tctgtttcta tcaaacgtat aatttgtata gaatctagaa ttatttcatc
     9901 aagaaaattt ttttctgatt taccaatctt gcctttttcg tacaaatctt ggactgattg
     9961 tattgcggga attatgactt cggtagtgat ttttatcgga tctgcacctg aatgaagcga
    10021 atttcgcagt atattatgtg cctctttatg agatgctgtc aagagatatt gaagatattt
    10081 gttcttgact tggaagaaat ctgccggaaa acgtagctgg tctactcctt cttctacaat
    10141 ccacagattt acatttccta tgtttctttg tctgattaat ccttctgctg caaagacgtt
    10201 aagatatttt gcaatggtga ttctactgat tccaagtttt tcagctattt ctatacctga
    10261 aagaccagtt tttgaatttt gtaagacatc aactagtttt tctttaattt tggtaatttg
    10321 ataaccgcga gccacgagtt ttcaaaatgt tctttctagt ataaagcatt acgaagaaat
    10381 aaccaacagg ggtcaaattg taggccccct gttggctcgg tactttggca tggtctgtag
    10441 ttcgttacga gcgcactaat gtgtataatg ctaattttgt aaataaaaag tgcgtatgaa
    10501 taataccaaa tacttaaaaa cttgatttga atttttgata aaaaataatt tgacaatcaa
    10561 atgcaaattg gtttcatttg attacaacag aataagattc acttgtaatg aatcagtgta
    10621 aaaatcacaa cattgtgtca gaacaagttt tatgtacaat catagccaga aacattaaat
    10681 acttgtgtac tttaaaatac ggtaacaggt tagtaaaatg ccaaaagcag gattcaaatc
    10741 aataacagtt tcagaaacag tctacaacaa gttccacgat atctacaaga agaacagaca
    10801 agaacttgca atgaagggca tcaatagttt tgcaggttac attactttca tgatggaaga
    10861 cctgatgcaa aaggatgaga cttttgcaag atatgctcca aagctagaaa agattgcagt
    10921 agacgacaac cgcgtagtta tcaaagacaa tatcaaaaat agaattgccg aagttgcaat
    10981 tcaaaatggc gaactttact gcatgttatg tgaagaaaaa gattgtttcc atgtaggttt
    11041 tgtattttca cttccagatg tttatgaagt tctaaatgca cgtggcgtaa agcatcagag
    11101 ataaaaaaag tgcaggaggt gggatttgaa cccacgaacc cctaagagac gggatcaccc
    11161 atgttttatg atcttaagtc ccgcgcgtcc aaaggctagg ccttctttgg ccaggctcgg
    11221 cgactcctgc aatttactag ctgaaagttg tggaaattaa acgttttatg aaaatttttt
    11281 ttcagaatca agtaaggtta ataaggtttc aacaaagcga acttgtcatg cttcgatagc
    11341 tcagcctggt agagcgttac cttggtaagg taaaggtcgc gggatcgaat cccgctcgga
    11401 gctttttaat aatttttaat caagagttca gaatgtccag ttctcttttt cgaatctgaa
    11461 tttatgaatc gatttgcact gatctctatt attttatcag aataatcttc aaaaagattc
    11521 aagacttcat ctgattttga atttgagagc attactttac aacctttgga atctagcctt
    11581 tgaaattcta gaaacagtct ttcttgatcc tcatatccaa agctatcatt tgtgtaacta
    11641 gtaaagttag aagtttggct tactggctgg tatggtggat caagatacac aaaatcattt
    11701 ttttgtgcat tctcaagtgc ttttggaaaa tcttgacatt ttattgaaat atcttttgaa
    11761 tgtaatgcat gacttactgc aacaaggttt tctttattca ctatgtttgg attttcatat
    11821 ttaccaagag gaacattgaa ttttccttta ctgtttactc ggtataatcc attaaaacag
    11881 gtcttgttta gaaagatcaa tcgagaaact ttctcaatct gactttttgg attgctttct
    11941 cttacttcat aatagtattc ttttggattt ttgaaatagt tatcagagtg agcttctaat
    12001 gcagagacta attcctcaac cctatctctg attgtaacat aagacaaaac caaattagaa
    12061 ttcaaatcag atacaaagca ttctaatttt gggttttcac tgatgagatg aaatagaact
    12121 gcacctcctc ccaaaaacgg ttcaaaataa gtatcaaatt tttttggcat gtattgagac
    12181 aatattggaa tgagttgtct ttttccacca gcccacttga caaatggttt tggtgcaagt
    12241 gatagaattt gcttgtattg ctgtttcaat agacaatact ttcaaaattt tatttcgcaa
    12301 cgtttgacaa taaaagatca cacgttgtgc aatatttttt aatctagaaa catgagacaa
    12361 acttttcaaa gtatagacta gctagataga aaataaagta aaaggaaagg gaagtttagt
    12421 cccacttaga ccaagctgcc atccatctgt atgtccacgt tgtcagctta catcctaatg
    12481 ctgccatcgt cggagctaat acagcacccg caccagcgcc aagagcaact ggactgttat
    12541 agaacttgcc tacttgcggt tctataggtg tcatgtatgg aggcaatgtc ggtcttggat
    12601 atttgaatgc agtctctaac gggtctgcga ccagtatgag attgatcatg ttgaacaatg
    12661 gcagagacat tccacagagc acaccgccca tcaatatgag agagtgcttg ttcttctttg
    12721 ttgcccagta taccaaatcc aacagcattg ctgatggaat ccaaactggt gtaacaatga
    12781 agtcatatgg atatcctaat gcaaaccatg ctccctttgc aacccatgta tacactgtca
    12841 ttatcagtgc atagtaagtt gcggttcctg gaactccagt aaacgtaagg tagtatgctg
    12901 caccaacaac gagcatgagt gtttgtgata cagagaatac cacaaaggac gtccaagccc
    12961 agtcagtgta gaatatgtag tctccagcgt taattgtcaa caatgtagag ttgactgcaa
    13021 ccactactat gaataagtag tgagtacatc gtcttagcca gaccattatc cagtagagaa
    13081 aacgtgtgat atataaaatt ttataccggg attttagtcg tggatcgata ctaaccagtg
    13141 tgttgtatca aaataataaa agaaatgtga atcggatatt tcttatccgt gaatgtacgt
    13201 gactatcagt actgttgcta cagctgtagc acctagtaca ccagctagta ttccattgct
    13261 gttttttgat gaaccttctt ctccgacctt tacgcagaag aggtaaccaa ttgcctcaat
    13321 gattgtcagt acaatgaatg cgtagtggcc acttggagct ggatatgccc atggataata
    13381 gaagtatcca acagcagtac ctgcaaatgt cgataaccat gcagcaagat acgaagcagt
    13441 atacaagccc atcatgttct cgacacgtct gccgatcatt ttgtttacgt cagaactaga
    13501 tccgccaccg ccagaaccga atccttttgg taaggtcatt tggcaatata ataatccgta
    13561 tacttttaag tctttcctga attgagagca aaaaattgca tatgctagag gtttttaaaa
    13621 ataagaaaaa agatgcgctt ttgcgcgtac ttttctatgg gatggatctg ctgtacagtt
    13681 tgccttcgag ggccatcttg tacatttcag caacatatgg attctcacct ggggtttctg
    13741 ctccaagttc gacgagtctt gcatatactc taactacata ggcaagtgcg cccatgaatg
    13801 caacgactac acccatatta aacatccagt ggtttggtac tgcgaagatt tcttctacat
    13861 accagaagtg ccacatctcg ttgacaccga ttgtaaacat cgtagccagg taaccaagaa
    13921 tggtcatctt caggccagtg ttcatggagt tgtttggacc cctcaagacc ggtacctttc
    13981 tatcatagat tgaaacagca ccccaaccta atggtaaagc cacgaagtgg ctgtagagcc
    14041 accagtgtgc tggtgtgaat gcactatctc tgatagaagt ttgatgcagc gaaccatcga
    14101 caaaattgtc gacttccact gatgcagctg tagatcccat tgagatgacg atcatccaaa
    14161 tcttcttcag tctttggatt tcgacttctt tcggaatcaa tgccggcatt tgtgccatgt
    14221 tggttgtgtt gtctgttgca tatataaagc atgaacttac aaatggcgtt ttttattcta
    14281 attcatttgt caaaactaga atatttttaa aaaacattag caaaatattt gtaaaacata
    14341 taaaaaacac aatatccaaa aaacactata gctatgataa acataagcgt ttatcattgt
    14401 tttatctcaa actgatattt tattgcgtac aatgagggca aaatatatat cgttggtctt
    14461 tctgctatgg aatagttacg ggataactat gatgaataaa aaactaatcg tagcagcact
    14521 tggtgtaatt gttgcactgg gaacagtcgg tccttccgta ggtcaattgc ttcaacaagc
    14581 agaggcacac ggtgtacagg cacaacttca gagtcgtttt atcagaattg aagatgaaac
    14641 attttcaaga caatcgctga atacaggcga aaccttgaca gtaaaaggca ctctggtaag
    14701 tctagtacag cgagacctaa gagcatggct ttctgtttac ggtgaatcaa caaacgcagg
    14761 taacagatgg gaattcattt cacgagatcc acccggaaac gtatttgaca taccaggaaa
    14821 cggtgtagta gactacacac ttgtagttaa agcactagaa gcaggagtct atcacatgca
    14881 cactcagctt aacatcgctt caatcggtcc aggacttggt ccaggacaga cagtagtggt
    14941 agaaggcgag aacattgtga aaccaattcc gtatgcaaat gttgcatacc agtcgataat
    15001 cattgcagtg ggctatgtag tcacatttgc aactcgaccg tggcaagtga tctaaaaatc
    15061 ccttcctcct tttcattttc tctaatatcc ctagcgtcga ctttacaaat caagtcagta
    15121 aaaatgacaa aatcattata acgctaacca tcagagttgt gatatgattt ggccaggaat
    15181 gggcgatcct gcaaaacgaa agcagaccat aaaattcctc atcataactg cagcagttgc
    15241 agcaggcgca tactttgcta gttttgcagt cacaggatct ctaagtgcaa atgacccgct
    15301 caagcaatgc atcaataata gagacataaa ctacaaaatc tctgccacgt ttgagctcat
    15361 agtggatggc ataaaagtag agattcctgc aaacgttgga aattctgacg agtgtaaaaa
    15421 atcactttac acaatttcaa atgatggagt gatttatgca gaatgggatg aaaaatatga
    15481 ttttgaaata ggtcatttcc tatggatata caaattccct cttcgcgaaa tggatgaatc
    15541 taaatcaaaa atatttgtaa acgacataca gtcgcctgac tttgtccatg ccaagttaga
    15601 aaatggttat cattacagag gagagtttgt atccaaggca gcagatacat caaaagaaca
    15661 cgactttttg ccaccagaat cctaagtcat taacaaaatt cacattaact gacttggaaa
    15721 aataaggaaa aagaatggaa tttaccagta cttaccgttg tctggtataa gaccgatgcc
    15781 ttctttctcg aagtgtttat cgagttcaga aacccatctt tcgcgtgtgt ctccgcccaa
    15841 accgtgtact ctcatccaga atgcaatcac tccaagaagg aatactgcaa acattatagt
    15901 tccaaatatg tagaccatca catcatagaa tagtgggtca taagctggac ctatctggcc
    15961 tgcaaagttg gacaggatac ctacgagcat accgaatata gcgctcatca tgtatgagaa
    16021 cgatttatgt gcgatatata catttccctt tgaatttaaa aataacgtaa gataaagcta
    16081 cgaaattaaa ataataaaaa gtaggaaaag atcttagttg gatttagcga atctaccgct
    16141 tctgcctttt agtacaaagg ctattgcgat tccaccgaat acggctccta cgattacaga
    16201 accgccaaca actccgtttg cgtcaaggta tggagtacca gaacctgtcg ctggattgtc
    16261 tgctgctttt tgtaccttgg ctttagccag ttccaactgt tcttctaatg tagtttgtcc
    16321 tcctccgcct acacccacgt attgtgctgc ggccattggt aggagtgatg atactacaaa
    16381 tagtaccagt acggttgcgc caattagtat gggtttattc atatcttgta ccttgtcaga
    16441 acatgactgg cattgatatt taacttttat gtcaaatatc aaattagttt tgcaaaatga
    16501 aggtaacgtt tatgttttgc cccaagacca tgcgacctat gggacgagtt cattcgcata
    16561 gacatggtca atcgcattcg atcagacccg ttacacctag ttctccaaca tgggtaaagc
    16621 aaagcccaca agaaatcgaa gaacttgttt taaaatatgc aaaagatggt ctgactacaa
    16681 gtgaaatagg tgtaaagctt cgcgatcaac atgcaattcc tcttacaaaa gtgataacaa
    16741 aaaaatctgt gacagaaatc cttgaacaaa agggaaccaa accagaaatg ccagaagatt
    16801 tgaacaacat tgtcagaaag gcattaggat tgcaaaagca cctaaagaca cacacatcag
    16861 atagaagaaa tgtgcgatct cttgaattgc ttgaggccaa agtacacaga ctgtcgtcat
    16921 attataaaaa aacaggacga atccccaaaa cttggaaata taagtcagtc attgctcaac
    16981 ttgagtaatg gcaaagcttg acggcgcatt atcttatttt catgatagag tttcagagtc
    17041 tataaaatct ggaaaaaata tttctgtaat aactcatctt gattgtgacg gcattacatc
    17101 tggaagtatt acaactaaag ctctaatcag atccggagca agatgtacag ttcgcactgt
    17161 taatgaattt agtagaaatc tgattgagaa attaaaaaaa gattctagag atctttacat
    17221 aattacagat ctgggtggag gatttgcaaa agacctagat gagatattgg aagataactg
    17281 gattgtagtt gatcatcacc aaattccaaa tgaagagatg gataacgaac gtgtgatcaa
    17341 tgcatggaag tatggcatag atggaggcaa agaagtttct gcgggaggca tgtcatattt
    17401 agtttcaaaa gcattacata gagaaaacac agatcttgca tggatcgcag tagtttctgc
    17461 acttggagac aggcaagacc aaggagagaa aaaatctttt actggaatca atctagaaat
    17521 tgcatctact gcaaagaaaa atgatcaagt ggaaattgat cttgacatac ttcttgtagg
    17581 aagagagact cgtccactcc cagatgcact tgcatttaca tcacatccat ttattgaggg
    17641 acttacatgg aatcgcgatg catgtctctc tttgttgaac tcttcaggaa tcaaactaaa
    17701 agatggaagt aggtggcgtg ttcctgctga gctaaatgag gatgaaaaaa gaaatctctt
    17761 gcagacgatt tccaaattta cgtctggaaa aaatgcaact gagataatgg atgagcttgt
    17821 aggctacact tacacacttt ctggagaaga caaacgcagc tttttgcgag atgctagaga
    17881 attttccaca atgctaaact cttgtggaag aattcgcaag gcaggagttg gagttgctat
    17941 atgcatggga gacaggacta aaatgcttga ggagggagag agcatcctcc tagaataccg
    18001 tacggttttg agaacataca tgaacactct tgcaagtgaa cgctggagag ttgtagataa
    18061 tggtcaatac ctgatggtaa atggagaggg tttggtttca
//
LOCUS       DATIDL010000050         2013 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrosopumilaceae archaeon isolate SMAG_U16342
            k141_2685273, whole genome shotgun sequence.
ACCESSION   DATIDL010000050 DATIDL010000000
VERSION     DATIDL010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935836
            Sequence Read Archive: SRR25158326
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosopumilaceae archaeon (soil metagenome)
  ORGANISM  Nitrosopumilaceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrosopumilales;
            Nitrosopumilaceae.
REFERENCE   1  (bases 1 to 2013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/04/2024 05:09:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,520
            CDSs (total)                 :: 1,474
            Genes (coding)               :: 1,474
            CDSs (with protein)          :: 1,474
            Genes (RNA)                  :: 46
            rRNAs                        :: 1 (5S)
            complete rRNAs               :: 1 (5S)
            tRNAs                        :: 43
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2013
                     /organism="Nitrosopumilaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2685273"
                     /isolate="SMAG_U16342"
                     /isolation_source="soil"
                     /db_xref="taxon:2202732"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /locus_tag="VLB45_02215"
     CDS             complement(<1..233)
                     /locus_tag="VLB45_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavoprotein"
                     /protein_id="HSD04549.1"
                     /translation="MRLVVGITGSTGIIYGIRMLEVLKKYKVEIHLIMTEWAKKCLAL
                     ETKYDLKYVKSLADSYSDDSNMAASVSSGTYKT"
     gene            complement(280..1197)
                     /locus_tag="VLB45_02220"
     CDS             complement(280..1197)
                     /locus_tag="VLB45_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HSD04550.1"
                     /translation="MKSINYVLLGDESIAKELGKKGTSTDLTIYDKKESEFVKTWTAP
                     TGFPEKIQPLFQAINMGEYVIFYVTKLDKFTGEQIIALDSLKKAEGILLHSYDVDRTK
                     LLSMIKGTVVEKYKLIELNDLKKEIDSMQPVSKDGTVKISIDHCFDVKGVGTVILGKV
                     TQGKAKTYDNLKLLPKGSDVLIKSIQMHDDPVDEALSPARVGLSVKGITPDDVQRGDV
                     LCMPGSVQTSQEITLEYTQNSFFKGELAENQMFLVNIGLQIRPAKIISIKPMKLSLGK
                     PAVFEKNDTCVLLKPESQTIRIVGSGPII"
     gene            complement(1250..1927)
                     /locus_tag="VLB45_02225"
     CDS             complement(1250..1927)
                     /locus_tag="VLB45_02225"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HSD04551.1"
                     /translation="MTSESYPVVYGTTSNMKMTSFTTRLQNAIDDVISFSGIYKNYCV
                     CIVDAVDSTKITASLPKIKTCKYYGIFLNSMAMIAKEFGASIVKNIGDSLLYYFPHTF
                     DVSRTDPFGDVLECGMEMIKLRSVINEKMFDAGLPPISYRISADYGSIMTAKSENSSI
                     NDIFGSPVNICAKINSLAKPNGMVIGSDLYQIVKSFPGYHFQSITSYSAGLKLQYPVY
                     CITSIPS"
BASE COUNT          594 a          370 c          337 g          712 t
ORIGIN      
        1 tctgtcttgt atgtgccact tgaaacactt gcagccatgt tagaatcatc agaatagctg
       61 tctgctagag attttacata ttttaaatca tatttggttt ctaaagcaag acactttttt
      121 gcccattcag tcatgataag atgtatttca actttgtatt ttttaagaac ctcaagcatt
      181 ctgataccat aaattattcc agtgctacct gtaattccaa caactaatct cattaattaa
      241 cacgcatcca caagtaaaga agaagttcgt ctattttagt caaattattg ggccactgcc
      301 aactattcgt attgtctgag actctggttt taagagaaca caggtgtcgt ttttttcaaa
      361 gactgctggc ttgccaagcg ataatttcat tggttttatt gagattattt ttgcaggtct
      421 gatttgcaag ccaatgttta ccagaaacat ttggttctct gctagttccc ctttgaaaaa
      481 ggagttttgt gtatattcta gtgttatttc ctgagatgtt tgtacagatc caggcatgca
      541 taacacatca cctctttgaa catcatcagg agttatccct tttacagaaa gtcccactcg
      601 tgctggagat agtgcttcat ccacaggatc atcgtgcatt tgaatggatt ttattaaaac
      661 atcggagccc tttggcaaaa gttttagatt atcatatgtc ttggctttgc cttgtgttac
      721 ttttcctagt attacagttc ctacaccttt aacatcaaaa caatgatcta ttgaaatttt
      781 aactgtgcca tcttttgaca caggttgcat agagtcaatt tcttttttta gatcattaag
      841 ctcaatcagt ttgtattttt ctactactgt tccttttatc atggaaagaa gttttgtcct
      901 atcaacatca tacgaatgta atagtattcc ctctgctttt ttgagactat caagtgcaat
      961 aatctgttcg cctgtaaatt tgtcaagctt tgttacataa aatatcacat attcacccat
     1021 gtttattgcc tgaaagagtg gttgtatctt ttcaggaaaa cctgttggag cagtccaagt
     1081 tttgacaaat tcagattctt ttttgtcata tatggtaaga tctgtggatg tgcctttttt
     1141 accaagttct ttggcaatgg attcatcccc aagaagaaca tagttaattg atttcatttt
     1201 aataccagat agaaattcgt ggttatactt gtaaaaaatc ttgtgtcgtt taggaaggaa
     1261 tactagttat gcaatatact gggtattgaa gtttcaaccc tgcagaatag gaagtgattg
     1321 attgaaaatg atatccagga aacgatttta caatttgata caagtcactg cctatcacca
     1381 tcccgtttgg cttagccagg gagtttattt ttgcgcatat gtttacaggc gaaccaaata
     1441 tgtcattaat agaagagttt tctgatttgg ccgtcattat acttccatag tcagcgctaa
     1501 tcctatagct tatcggaggc aaaccggcat caaacatctt ttcattaata acagatctca
     1561 atttgatcat ctccatacca cattcaagca catcgccaaa agggtctgtt ctagaaacat
     1621 caaacgtatg tggaaaataa tacaacaaac tatctccaat gttttttaca atagatgcac
     1681 caaactcttt tgcaatcatt gccatcgagt taagaaagat gccataatac ttgcatgtct
     1741 ttatttttgg cagtgatgca gttatcttag ttgaatctac tgcatcaaca atgcatacac
     1801 agtagttctt gtaaattcca gaaaaggaga ttacatcatc aattgcattt tgtaaacggg
     1861 ttgtaaaact ggtcattttc atatttgatg tggtgccata tacaacagga taggattcac
     1921 ttgtcatgat tgcagttatc tttacgcttt gataggatct aattagctaa aattacaagt
     1981 acttagcttc actgctaaat ctgactaata ttt
//
LOCUS       DATIDL010000051         2397 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrosopumilaceae archaeon isolate SMAG_U16342
            k141_2078734, whole genome shotgun sequence.
ACCESSION   DATIDL010000051 DATIDL010000000
VERSION     DATIDL010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935836
            Sequence Read Archive: SRR25158326
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosopumilaceae archaeon (soil metagenome)
  ORGANISM  Nitrosopumilaceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrosopumilales;
            Nitrosopumilaceae.
REFERENCE   1  (bases 1 to 2397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/04/2024 05:09:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,520
            CDSs (total)                 :: 1,474
            Genes (coding)               :: 1,474
            CDSs (with protein)          :: 1,474
            Genes (RNA)                  :: 46
            rRNAs                        :: 1 (5S)
            complete rRNAs               :: 1 (5S)
            tRNAs                        :: 43
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2397
                     /organism="Nitrosopumilaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2078734"
                     /isolate="SMAG_U16342"
                     /isolation_source="soil"
                     /db_xref="taxon:2202732"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            132..1340
                     /locus_tag="VLB45_02230"
     CDS             132..1340
                     /locus_tag="VLB45_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04552.1"
                     /translation="MDERMISYKIPILIGVFLLLPFAYAESIPDYYNPYAPIYTDKQV
                     YSWTDKIYITIDAPSWNENTHGVDSIGDEQGYFVKISTASHKLEPYKLVETGPNTGKF
                     VGEVTLTGFSHDADGDGQIDTTPRTGGNGPTSGFLEADRDDGLSISFEFADGVVLTQN
                     AKVSWNHGNIAFDKKSYMLAESAQIRVIEPDMNLNPESSDTIHVEISSDSDTAGIEID
                     AVETDESSGIFEAEISFTQTGSSSGNRLYAVPDDVIYAKYEDNTLPSPYSTNDHLDVI
                     AESILEVDLQSGPKVDFKNLAVVDNSGTIIEKPMIGQQLQISAEIQNTQSYEQPFAYL
                     VQVKNSNGIVVSLSWFEGSLVTDQQLQVSQSWIPADLGTYNVETFVWKSLKNPFPLAK
                     PLTLEYKITQ"
     gene            1487..2251
                     /locus_tag="VLB45_02235"
     CDS             1487..2251
                     /locus_tag="VLB45_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008301601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6293 family protein"
                     /protein_id="HSD04553.1"
                     /translation="MQTIHTMMTSLTNLRIHIAPVGFEIDRIVIPAKKMKADKVCLLM
                     HGDPSKDKAKGYMEKIRAELKKEKIDVEVVYSDRFDLFKILKSVKEIVQKEKENGIFV
                     NLSSGSKIQAIACMMACMMFQGKNKLTPYYAEPENYATVKGAQLSSGLKTIVQLPAYE
                     IHTPKPVLVQALKIIREHGGKITKKEMARLAHENKLIVVNAREENFQQARFASLDKNI
                     IQPLLRDWKFVEIEQIGRNRWITITPEGLGAEEFLS"
BASE COUNT          859 a          444 c          470 g          624 t
ORIGIN      
        1 gacattgcag aaaaacagat cctgtcacct gaagaagaaa aagaaatgta tgaagagttt
       61 agagcagtca acgctgtcat caccaagcta gatgaaatgg taaaatcaat aaattctcta
      121 gaagaaaaat catggatgaa agaatgatct catacaaaat tccaatattg attggagtgt
      181 ttttgctatt gccttttgca tatgcagaat caattcctga ttattacaat ccgtatgctc
      241 cgatatacac tgacaagcaa gtgtacagct ggacagacaa gatctacatt acaatagatg
      301 caccaagctg gaatgaaaac acacatggcg tagattccat tggagacgaa caaggctatt
      361 ttgtaaaaat atccacagcc agtcacaagc ttgaaccata caagctagta gaaacaggac
      421 caaacacggg aaaatttgta ggcgaagtaa ctcttactgg gttttcacac gacgctgatg
      481 gcgacggtca aattgatacc acgccaagaa ctggaggaaa tggaccaaca agcggttttt
      541 tggaagctga cagggacgac ggactaagca tatcgtttga gtttgcagat ggtgttgtac
      601 taacacagaa cgcaaaggta agctggaacc atggaaacat agcctttgac aagaaatcct
      661 acatgttagc tgagagtgca caaattcgcg taatagagcc tgacatgaac ctaaatcccg
      721 agtccagcga tacaatacat gttgagatat catcagattc tgatactgca ggaatcgaaa
      781 tagatgcagt agaaacagat gaaagctctg gaatctttga agcagaaata tcatttacgc
      841 aaactggttc atctagcggc aacaggttgt atgcagttcc agatgatgtg atatatgcaa
      901 aatatgaaga caatacactt ccatcgcctt acagtactaa tgatcatctg gatgttatag
      961 cagaatccat actggaagtt gatctacaat ctggaccaaa agttgatttt aaaaatctag
     1021 ctgttgttga taattctgga acaataatag aaaagccaat gatagggcaa caattacaaa
     1081 tatctgcaga aattcaaaac acgcaaagct atgaacaacc atttgcatat cttgtacagg
     1141 taaaaaattc caatggtatt gtggtatcac tttcttggtt tgaaggatca cttgttacag
     1201 atcaacaatt acaggtttca caatcatgga tccctgcaga tcttggtaca tacaacgtag
     1261 aaacttttgt ctggaagtcg ctcaaaaacc ccttcccgct tgctaaacca ttaacactag
     1321 agtacaaaat aacacaatag aatttttctt acgaaatctt attcctttgt ttcaaaaatt
     1381 gattttactt taggaaatac atgaacttca atttcgtttt ctagcttgca cactgctttc
     1441 atacctgcat tagagttgca gaattattat atatgatgca aatagtatgc aaaccatcca
     1501 taccatgatg actagcttga cgaatttacg catacatata gcgcctgtgg gctttgaaat
     1561 tgacagaatt gtaatacctg ccaaaaaaat gaaggcagac aaggtctgtc ttttgatgca
     1621 tggcgatccg tcaaaagaca aggcaaaagg atacatggaa aaaattcgtg ccgagctcaa
     1681 aaaagaaaag atcgatgtcg aggttgtata ctctgatagg tttgatctat tcaagattct
     1741 aaaatcagtt aaagaaatag ttcaaaaaga aaaagaaaac ggcatttttg taaatctctc
     1801 ttctggctca aaaattcaag ctatagcatg catgatggcg tgcatgatgt ttcaaggcaa
     1861 aaacaaactt accccatact atgcagaacc tgaaaactat gcaacagtaa aaggtgcaca
     1921 gctctcttct ggattaaaaa caatcgtcca gctgccagca tatgaaattc ataccccaaa
     1981 acctgttctt gtacaggcac tgaaaataat aagagaacat ggaggcaaaa taaccaaaaa
     2041 agaaatggca aggcttgccc atgaaaacaa gcttattgta gtaaacgcaa gagaagaaaa
     2101 ctttcagcaa gcaagatttg catcacttga taaaaacata atccagcctc ttttgaggga
     2161 ttggaagttt gttgaaattg aacaaatagg aagaaaccgc tggatcacaa tcactcctga
     2221 aggtcttggc gcagaagagt ttctatctta ggtagttagc tcaggtttca gagtccatgc
     2281 tactgcaagt attacaaatg gaattgagat catgacggta atttgcaaaa ttataaacaa
     2341 ctgggtctgg gccacgcttg ggtttcctat aatgaatggc aacggtaagg tgattat
//
LOCUS       DATIDL010000052         2094 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrosopumilaceae archaeon isolate SMAG_U16342
            k141_1070517, whole genome shotgun sequence.
ACCESSION   DATIDL010000052 DATIDL010000000
VERSION     DATIDL010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935836
            Sequence Read Archive: SRR25158326
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosopumilaceae archaeon (soil metagenome)
  ORGANISM  Nitrosopumilaceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrosopumilales;
            Nitrosopumilaceae.
REFERENCE   1  (bases 1 to 2094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/04/2024 05:09:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,520
            CDSs (total)                 :: 1,474
            Genes (coding)               :: 1,474
            CDSs (with protein)          :: 1,474
            Genes (RNA)                  :: 46
            rRNAs                        :: 1 (5S)
            complete rRNAs               :: 1 (5S)
            tRNAs                        :: 43
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2094
                     /organism="Nitrosopumilaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1070517"
                     /isolate="SMAG_U16342"
                     /isolation_source="soil"
                     /db_xref="taxon:2202732"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..594)
                     /locus_tag="VLB45_02240"
     CDS             complement(<1..594)
                     /locus_tag="VLB45_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04554.1"
                     /translation="MMRKLSMLFMIFFIGQLFVISAHGQFTKSDSPFEREFADIKFLD
                     AYFGTFEQKVEVDAGDQNVPLTVVMANIGTQDITGIRGQLSLPSGFSPSDGKGALILA
                     DKDDNSLAGDVFSLTFFVNVDKNLDVGQYPGTIKVDYSRLRETGQRNMFFNFDFKLTG
                     DSTLNIKAIDPILTSIKNNLVVVEVSNLGTAPISNVDI"
     gene            complement(591..1265)
                     /locus_tag="VLB45_02245"
     CDS             complement(591..1265)
                     /locus_tag="VLB45_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSD04555.1"
                     /translation="MTKIYGEGETAVHALRDVSFSVKKGELLLIVGSSGSGKSTLLNM
                     IGLLDRPTSGKVFVDGTDTSTLNDSKLSSFRNSKLGFIFQFANLLSDLTVLENVTLPR
                     KIQGTNDVVDEAKKLLRTVGLEKQMNNRANKVSGGQAQRAAIARGLINNPSIVLADEP
                     TGNLDSVTAEVIVQLMKSLVKKLGQTFIIVTHDRQQFGDVDRVITIKDGRAFEGTEQK
                     GMEVTV"
     gene            complement(1288..>2094)
                     /locus_tag="VLB45_02250"
     CDS             complement(1288..>2094)
                     /locus_tag="VLB45_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012216096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HSD04556.1"
                     /translation="IHDTVGDGQYVFARNSIVVGSRVARDLGGVEVGDNIKLKFTDRH
                     GEDQLKRFVVTGITSSAGGQGLDSSVIVHIDTLRSLLDRNDETGAILVKLNDPTKAVE
                     IRDFFLRSFPTDDFKAETIEESAETQLAGFRSGIALINLIGYFGMMSSAFAIVTIQMM
                     LVSSKTREIGIMRAIGAKRKDILILFLFQGMIIGAIGAAVGTGFGLAYTFYAKETKMQ
                     FEGSLALEVTYNWEKITQTAFTSFGLAIMASIYPAYRATKLLPVEAMRAV"
BASE COUNT          609 a          446 c          334 g          705 t
ORIGIN      
        1 aatgtcaaca tttgatattg gtgcagtacc caagttagaa acttcaacaa ctacaagatt
       61 attctttatt gatgtcaaaa taggatctat tgcctttatg ttaagagtac tgtctcctgt
      121 aagtttgaaa tcaaaattaa aaaacatatt tctttgtcca gtttctctta gtctagaata
      181 atctactttt attgttcctg ggtattgtcc aacatccaaa ttcttgtcta cgttaacaaa
      241 aaacgttagt gaaaatacat cacctgcaag ggaattatcg tctttatctg ccagaatcag
      301 agcacctttg ccatctgatg gagaaaatcc agatggtaat gacaactgtc ctcttattcc
      361 cgtaatatcc tgtgttccta tatttgccat aactacagtt agtggcacat tctgatctcc
      421 tgcatccact tcgacttttt gttcaaaagt gccaaaatat gcatcaagga actttatgtc
      481 agcaaattct ctttcaaatg gagagtctga tttagtaaat tggccatgtg cacttatgac
      541 aaaaagctgt ccaatgaaaa atatcataaa cagcatagat aattttctca tcatacagtt
      601 acctccattc ctttttgttc tgttccttca aaggcacgac catcctttat ggtgatgact
      661 ctatccacat caccaaattg ctgtctatcg tgtgtaacta taatgaatgt ctgtcctagc
      721 ttttttacta gagatttcat gagttgtaca attacttctg cagttacaga atccaagttg
      781 cctgtaggtt catcagcaag tacaattgag ggattgttaa ttagacctct agctatggct
      841 gctctttgag cttgaccacc ggatactttg tttgcgcggt tgttcatttg tttttcaaga
      901 cccactgttc ttagtaattt tttagcttca tctacaacat cattggttcc ctggattttt
      961 cttggaagag tgacattttc taaaactgtt aagtctgaca agagatttgc aaattggaaa
     1021 ataaatccta atttagaatt tctaaaagat gataatttgc tatcattaag tgtcgatgta
     1081 tcagttccat ctacaaagac tttaccgctt gtaggacgat ccaacaatcc aatcatgttt
     1141 agtaatgtgg atttaccaga acctgagctg cctacaataa gaagcagttc tccttttttg
     1201 actgaaaacg atacgtctct taatgcgtgt actgccgttt cgccttctcc gtatattttg
     1261 gtcaaatttc taacttctaa aaccaaatca gacagccctc atcgcctcca ctggtaaaag
     1321 tttggttgct cgatatgcag gatatattga agccattatt gccaatccaa aagaggtaaa
     1381 agcagtttgt gttatctttt cccaattgta tgtaacttcc aaggccaaac ttccttcaaa
     1441 ttgcatcttt gtctcttttg catagaaggt ataagctaaa ccaaaacctg ttcctactgc
     1501 tgcgcctatt gctcctatta tcattccctg gaacaagaat aaaatgagaa tgtcctttct
     1561 ttttgcgcct attgctctca tgattcctat ttctcgggtt ttgcttgata caagcatcat
     1621 ctgaattgtt acaatggcaa acgcagatga catcattcca aaatatccaa tgagatttat
     1681 caaagcaata cccgatctaa aaccagccag ctgtgtttca gcagactctt ctatggtctc
     1741 agctttaaag tcatcagttg gaaacgatct caaaaagaaa tctctgattt ctactgcctt
     1801 tgtgggatca tttagtttta ctagaattgc tccagtttca tcatttctgt caagcaaact
     1861 acgtaatgta tcaatatgaa caataacgct gctgtctaat ccttgtccac ctgcagaaga
     1921 ggttattcca gtcacaacaa aacgtttgag ctgatcctcg ccatgtctat ctgtaaattt
     1981 tagttttatg ttatcaccaa cttccactcc tccaagatca cgtgcgaccc gagaacccac
     2041 cactatagaa tttcttgcaa atacatattg accatcacct actgtatcgt ggat
//
LOCUS       DATIDL010000053         2442 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrosopumilaceae archaeon isolate SMAG_U16342
            k141_1276317, whole genome shotgun sequence.
ACCESSION   DATIDL010000053 DATIDL010000000
VERSION     DATIDL010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935836
            Sequence Read Archive: SRR25158326
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosopumilaceae archaeon (soil metagenome)
  ORGANISM  Nitrosopumilaceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrosopumilales;
            Nitrosopumilaceae.
REFERENCE   1  (bases 1 to 2442)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2442)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/04/2024 05:09:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,520
            CDSs (total)                 :: 1,474
            Genes (coding)               :: 1,474
            CDSs (with protein)          :: 1,474
            Genes (RNA)                  :: 46
            rRNAs                        :: 1 (5S)
            complete rRNAs               :: 1 (5S)
            tRNAs                        :: 43
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2442
                     /organism="Nitrosopumilaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1276317"
                     /isolate="SMAG_U16342"
                     /isolation_source="soil"
                     /db_xref="taxon:2202732"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..656
                     /locus_tag="VLB45_02255"
     CDS             <1..656
                     /locus_tag="VLB45_02255"
                     /inference="COORDINATES: protein motif:HMM:NF014856.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosylglycinamide synthetase C
                     domain-containing protein"
                     /protein_id="HSD04557.1"
                     /translation="KRLFPGDIGPSTGEMGTSMFWSQQNKLLELTLEKMKQKLIESKY
                     VGYIDINCIANGKGVFPLEFTSRFGYPTISIQMEGITSNLGDLFYKMAGGEQVDIKTK
                     KGFQVGVVIAVPPFPFTDDRTFRKYSEEATILFKKENKEGVHIGEIKRSNDDWHVAGK
                     SGYALVVTGSGTSMEDARKQAYHRVENVMIPNMFYRTDIGARWINDSDMLLSWGYLY"
     gene            complement(648..902)
                     /locus_tag="VLB45_02260"
     CDS             complement(648..902)
                     /locus_tag="VLB45_02260"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HSD04558.1"
                     /translation="MVTAFVMISCNTGQDEHVIDKLKTMIPVREIHSTVGVYDIVTKV
                     EANTADELKNAITAKIQNLSNVRSTLLLMKSRSQHGQTSV"
     gene            1078..1305
                     /locus_tag="VLB45_02265"
     CDS             1078..1305
                     /locus_tag="VLB45_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HSD04559.1"
                     /translation="MSQAFLLISCDLGKEGKVVKELEAIDDVKEVQQTTGVFDIIAKI
                     ESDSDKKLKETVSSKIRNIDPVRSVLILEAD"
     gene            1518..1859
                     /locus_tag="VLB45_02270"
     CDS             1518..1859
                     /locus_tag="VLB45_02270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04560.1"
                     /translation="MKPTFVVASQNFERDLSILKDILNQLELKTSRKDSYKFAEKGQK
                     AAGWWFYEIYCKSDFMDEIIASEAADKIESDRKVLEIIQDKLKQNNSNARIRLYKDKP
                     FLSRWWIWLMS"
     gene            1923..>2442
                     /locus_tag="VLB45_02275"
     CDS             1923..>2442
                     /locus_tag="VLB45_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010189493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:calcium antiporter"
                     /protein_id="HSD04561.1"
                     /translation="MSELIINIALLAVFLVILVRSAMFAIDSIVKFSRRTRISELAAG
                     FVIVAVATSTPEISVAIFSTYSDNVGITLGDIFGSNVTNIALITALFLLVSPVKQISQ
                     ATLRRFFPLLVAASLIPLLLLLVQQGTVLIGMALLAAFAFFIYRTFKSSAKETKEHEP
                     GSPYKPLLFFFIG"
BASE COUNT          817 a          436 c          444 g          745 t
ORIGIN      
        1 acaagcgtct tttccctggt gatataggcc caagtacagg agaaatggga acatcgatgt
       61 tttggagcca acaaaacaag ctcttagagc ttacgctgga gaaaatgaaa cagaaattaa
      121 tagaatcaaa atatgttgga tatatagaca taaactgtat tgcaaacggt aaaggcgtgt
      181 ttccattaga gtttacttca agatttgggt atccaacaat atcaatacaa atggaaggaa
      241 tcacaagcaa cttgggagat ctcttttaca aaatggctgg aggagaacaa gtcgatataa
      301 agacaaaaaa aggatttcaa gttggtgtag taatagcagt tccgcctttt ccatttactg
      361 atgacagaac ttttagaaag tactctgagg aagctaccat acttttcaaa aaagaaaaca
      421 aggaaggagt acacattggc gaaataaaga gatcaaacga tgactggcat gtggcaggca
      481 agtctggtta tgcacttgta gtaacaggat ctggaaccag catggaagat gcaagaaaac
      541 aggcatacca cagggttgaa aatgtgatga ttccaaacat gttttacagg acagatatag
      601 gcgctagatg gattaacgac agcgatatgc ttctctcgtg gggctatcta tactgaagtt
      661 tgtccatgtt gtgacctgct cttcatcaat agaagtgtag aacgaacatt gctcaaattt
      721 tggatttttg ccgtaattgc attttttagc tcatctgcag tatttgcctc tacttttgtt
      781 acaatgtcat aaacacctac tgtagaatga atttctctta ctgggatcat tgtttttagc
      841 ttatcaatga catgttcatc ctgtccagtg ttgcaactaa tcattacaaa tgcagttacc
      901 atgattttat tacgatctga tcatatttaa aaccacgcca aaattaaaaa atatttgatt
      961 ttttgaaatc agatatttta tttgcatctt gtgtacaaaa atttttttgg cacaaattaa
     1021 aataaaatta acaaattaat cacatcattg aaatactttg tttgctataa taataacgtg
     1081 tcacaggcat ttcttcttat ttcatgtgat ctaggaaaag aaggcaaggt tgtaaaagaa
     1141 cttgaggcca tagacgatgt aaaagaagtt caacaaacca cgggtgtatt tgatattatt
     1201 gcaaaaatag aatctgattc tgataaaaaa ctaaaagaaa cagtatcctc aaagattcgt
     1261 aacatagatc ctgtacggtc tgttctaatt ttggaagcag actgaataca gtaaatccaa
     1321 ctgtcatgtt ttctgaaaac atagtttaaa gatgaaccct ataattatcc gtcttgtgat
     1381 tttggtgcct tcaattataa ttacaatttg ggcagtttac acaattctaa caaaatcatt
     1441 tccataacga ccaattgatt ttcatgcatg aaattctgat atccacttta taactggaat
     1501 caaaccaaca ccaccaaatg aaacctactt ttgttgtggc atcccagaat ttcgagcgtg
     1561 atttgtccat tttaaaagac attctaaacc aattagaact gaaaaccagc cgcaaggatt
     1621 cttacaagtt tgctgaaaaa ggtcaaaaag cagcaggttg gtggttttat gaaatttatt
     1681 gcaaatctga tttcatggat gaaattatag catcagaagc tgcggataaa atagaaagtg
     1741 atagaaaagt tctggaaatc attcaagata agctaaaaca aaacaactcc aatgccagaa
     1801 taagattgta caaagacaag ccgtttttga gccgctggtg gatctggctt atgagttgat
     1861 catttacgat actatatcgt tatattcaaa attctcaaat gccttaaccg ttctaatact
     1921 ttatgtctga attaataatc aacatcgcct tactggctgt ttttttggta atacttgtcc
     1981 gaagtgccat gtttgcaatc gattcaatag taaagttttc aagaagaacc agaattagcg
     2041 agcttgcagc aggatttgta atcgtggctg ttgcaacctc gacaccagaa atcagcgttg
     2101 caatattttc cacatattct gacaatgtgg gaataacgct tggcgatatt tttggctcta
     2161 acgttacaaa cattgcacta attacagcgc tctttctttt ggtatctcca gtaaaacaga
     2221 tatcgcaagc aacactgcgc agattctttc ctcttcttgt tgcagcctcg cttataccac
     2281 tccttctttt gcttgtgcag caaggaaccg tattaatcgg aatggcactg cttgcagctt
     2341 ttgcattttt tatctaccgt actttcaagt caagtgcaaa agaaacaaaa gagcatgaac
     2401 caggctcacc ctacaaaccg ctgctatttt ttttcattgg ta
//
LOCUS       DATIDL010000054         4410 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrosopumilaceae archaeon isolate SMAG_U16342
            k141_2893765, whole genome shotgun sequence.
ACCESSION   DATIDL010000054 DATIDL010000000
VERSION     DATIDL010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935836
            Sequence Read Archive: SRR25158326
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosopumilaceae archaeon (soil metagenome)
  ORGANISM  Nitrosopumilaceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrosopumilales;
            Nitrosopumilaceae.
REFERENCE   1  (bases 1 to 4410)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4410)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/04/2024 05:09:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,520
            CDSs (total)                 :: 1,474
            Genes (coding)               :: 1,474
            CDSs (with protein)          :: 1,474
            Genes (RNA)                  :: 46
            rRNAs                        :: 1 (5S)
            complete rRNAs               :: 1 (5S)
            tRNAs                        :: 43
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4410
                     /organism="Nitrosopumilaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2893765"
                     /isolate="SMAG_U16342"
                     /isolation_source="soil"
                     /db_xref="taxon:2202732"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..705)
                     /locus_tag="VLB45_02280"
     CDS             complement(<1..705)
                     /locus_tag="VLB45_02280"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HSD04562.1"
                     /translation="MIPLRDENPKPAGYRPKMTYALIAINAIIFLWEVASTGQIFEFT
                     NQRAAVLFHQYGTVPTDIIEGLIDRDYGVIAPLFTSMFLHAGIMHIGGNMLFLYIFGD
                     NIEYRFGRGKYLILYLFWGLVAGFAHIMYDPSSPIPAIGASGAISGVLGAYLIMYPNA
                     KIMTVMFFGFFMRMLHISAKWYLPFWFIFQNLLPLLIGSSGSGVAFLAHIGGFVIGLL
                     TGYIYKKTHRSEFTYGT"
     gene            complement(874..2292)
                     /locus_tag="VLB45_02285"
     CDS             complement(874..2292)
                     /locus_tag="VLB45_02285"
                     /inference="COORDINATES: protein motif:HMM:TIGR04296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEFG-CTERM sorting domain-containing protein"
                     /protein_id="HSD04563.1"
                     /translation="MQFIPKGIPLLVAFTLFSFLVVFVSYDAYGDGLTMETLPPASLG
                     DRQASLFIQVNPPILTSDTAQDTFVKLRLFDANNNQTIQSNSFFVEVTKDDELLMREL
                     FYTKSGFLTIKFKPTDTPGKWQAYGDIEPTLGGWTATNDEVRVDAPVFLEGGLYHFEI
                     KFIGVDAAKNIFAQGNEPTFNSYLSVGDVFKTPIKYQAKSYDAEIISYYDRIQDFEFD
                     ESKKLVSFSMPFNWDVSRFENQNIFVHEEIQIPKSFKEFTDTNTFTAKVNGFPLVGRM
                     LAVDPYSKEDVLILHYLINKNDILDLAKNVSPDVKTMDFTLTPGFADAQTSTEILTDF
                     GGFRAELEWSPADLKANTKADLKVTFYDAFSGGQVAGDVKYDLKILDKNGNAAFSKTD
                     LDAIGGADTQSITFANDDVYTIEIDIKSITINEKTDSSRLGIARGVVVVPEFGSIAIL
                     VLIIAIGTIIATRIVPIRFRTI"
     gene            2359..3240
                     /locus_tag="VLB45_02290"
     CDS             2359..3240
                     /locus_tag="VLB45_02290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04564.1"
                     /translation="MGNENKIRKMNGWKKVSFILGVFALGLTIYFSGFYTQDNESLAY
                     EHDTPITDTISDDPVENTIKDITYTAKFVCGSVFGSEGPLRPGHYDSDISIFNRQNFP
                     ITILWNAVINDGTSSNAIIKKLEPETSTGIVCKEIRQLFDDNSEKIVEGFVIIRIQLD
                     SVISSEGDTLIRNLSASEINLLDVQVFYTANALPTLPHEIIVDKISFYIIQDGTDKIP
                     KEMMRKPLDVSIPSGLNEISNTEDRIKDILAEKYNLVEEDLTKIVLRIKDVSVGVGAL
                     IDDHAISLHVVTPQANS"
     gene            3362..4306
                     /locus_tag="VLB45_02295"
     CDS             3362..4306
                     /locus_tag="VLB45_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CFI-box-CTERM domain-containing protein"
                     /protein_id="HSD04565.1"
                     /translation="MALPVYAQEQSEETVLPTDKGTINVGLSTIPDQPNPSQETKLKI
                     NFLNKNTNMIQEHIDYSITVKKGESQVFSIPLTHTAIGSVTIPYQFEIAGQYQVSVDI
                     QGILFQPIPTESAVFSISVVDSQSKNGCLIATAAFGSELAPQVQFLREYRDNTVMTTV
                     AGSSFLNVFNAVYYSFSPTVADVERNNPFLQESVRIGIMPLLGILQVTETLNTGGEIS
                     VVISGIIASLLVGAIYLWPAGLITRAYRKSLRSSTKFVIIIISIVIALTLISIATANV
                     QFMAISTSIMILALAGAGAIFSSQAIWKAVLKIRSTFL"
BASE COUNT         1451 a          785 c          779 g         1395 t
ORIGIN      
        1 tgttccatat gtaaattctg atctgtgtgt ttttttgtaa atatatcccg taagtaaacc
       61 aattacaaat ccaccaatgt gtgccagaaa agcaacacca gaaccacttg atccaataag
      121 aaggggcaaa agattctgaa aaataaacca gaaaggaaga taccactttg cagatatatg
      181 caacattctc atgaaaaacc caaaaaacat aacagtcatt atttttgcat ttggatacat
      241 aatcaaatat gcacctagta cgccagaaat tgcacctgat gcacctatag ctggaatcgg
      301 acttgatgga tcatacataa tgtgcgcaaa cccagctact agtccccaga aaagatacag
      361 tattagatat tttcctcttc caaatctgta ttcaatgttg tctccaaaaa tatacaaaaa
      421 caacatgttt cctccaatgt gcattattcc agcatgaaga aacatcgaag tgaaaagtgg
      481 tgctataaca ccatagtctc tatcaatcag tccttctatt atgtcagtag gaactgttcc
      541 atactgatgg aacaacactg cagctctttg gtttgtgaat tcaaagattt gcccagttga
      601 tgcaacttcc cagagaaata ttatcgcgtt tatagcaatc aaggcataag tcatctttgg
      661 tctgtagcct gcaggctttg gattctcatc acgtagaggg atcattggat aataatttca
      721 cgcagaaatt tgtataataa gatttgtcaa ttttgaaaat ccatacaaaa attattccta
      781 ctttaatcac acattggatt cctgacgcaa agtacaaaca gactaaaatg caatagagag
      841 ttttgctaac gctgactaat tttagataag tttctagata gttctaaatc ttatcggaac
      901 aattctagtt gctatgatag taccaattgc tattatcaaa accagaatgg cgattgatcc
      961 aaactctggc actaccacta cgcctctggc aattcccagt ctgcttgaat cagtcttttc
     1021 attaattgta atcgatttga tgtctatttc tatggtataa acatcgtcgt ttgcaaaggt
     1081 aattgattga gtatcagcac cgccgattgc atcaagatca gtttttgaga atgcagcatt
     1141 gccatttttg tccaatattt ttaaatcata ttttacatca cctgcaacct gtcctcctga
     1201 aaatgcatca taaaatgtca ctttgagatc ggctttggtg tttgccttca aatcagctgg
     1261 agaccactca agttcagctc ggaatccacc aaagtcagtc aatatttcag ttgatgtttg
     1321 tgcatctgca aagcctggtg tgagtgtaaa gtccattgtt tttacatctg gagaaacatt
     1381 ttttgccaga tccaagatat catttttgtt aatcaaataa tgcagtatga gtacgtcctc
     1441 tttcgaatat ggatctactg caagcattct tccaacaagt ggaaaaccat ttactttggc
     1501 agtaaatgta tttgtgtctg taaattcttt gaatgatttt ggaatttgga tttcttcgtg
     1561 tacgaaaata ttttgatttt caaaccttga tacatcccag ttaaacggca tagaaaatga
     1621 aactagtttt ttggattcat caaattcaaa atcctgtatt ctgtcataat atgaaattat
     1681 ttcagcgtcg taagacttgg cttgatattt aattggtgtt ttaaaaacat ctcccacact
     1741 caggtaggaa ttaaatgttg gttcattacc ttgtgcaaaa atattcttgg cagcatcaac
     1801 gccaatgaat tttatttcaa aatgatacag tcctccttct aaaaagactg gagcatccac
     1861 tcttacctcg tcattagttg cagtccatcc gcccagagtt ggttctatat ctccataagc
     1921 ttgccacttt ccaggcgtat cagttggttt gaatttaatt gttaaaaatc cagattttgt
     1981 gtagaacagc tctcgcatca aaagttcatc atcttttgtt acttcaacaa aaaatgagtt
     2041 ggactggatt gtctgattgt tatttgcatc aaacaatctg agcttcacaa atgtatcttg
     2101 tgcagtgtca gaagtcagaa ttggtggatt gacttggatg aaaagacttg cctgtctgtc
     2161 acctaggctt gcggggggta gagtttccat cgtaagacca tccccatatg catcatagga
     2221 tacgaatacg actagaaatg aaaaaagtgt aaacgcgaca agtaatggga tgccctttgg
     2281 aataaactgc atacaattga gaacactaag aagattctat ttaattctta gtcagattct
     2341 tgctaatggt ataaatgatt gggaaatgaa aacaaaataa gaaaaatgaa tggctggaaa
     2401 aaagtttctt ttattttagg tgtatttgca cttggattga ctatttattt ttctggtttt
     2461 tatacgcaag acaatgaaag tttagcatat gaacatgaca caccgatcac tgatactatt
     2521 agtgatgatc ctgtagagaa tacaataaag gacataacat atactgcaaa atttgtttgc
     2581 ggttctgtct ttggaagtga gggtccacta aggccaggtc attatgatag cgatatcagc
     2641 atatttaaca gacaaaattt cccaatcaca atactttgga atgctgtaat aaatgacgga
     2701 acaagctcaa atgcaattat aaaaaaatta gaaccagaaa cgtcaactgg catagtttgc
     2761 aaagaaatca gacaattatt tgatgataat agtgaaaaga ttgttgaggg atttgtgata
     2821 attagaattc agctggattc tgtaatatca tcagaaggag acacattgat aagaaatctt
     2881 tcagcaagtg agatcaatct tcttgatgtt caagtgttct atacggcaaa tgctctacct
     2941 actcttccgc atgaaatcat agtggacaag atctcgtttt acataataca ggatggtact
     3001 gacaagatcc caaaagaaat gatgcgtaaa ccacttgacg tttctatacc atcaggacta
     3061 aatgagatat caaatactga agatagaata aaggacattt tagcagaaaa atacaattta
     3121 gttgaagagg acttgacaaa aatcgtactt cgaataaagg atgtttctgt tggggttggc
     3181 gcattaatag atgatcatgc catatcatta cacgtagtaa caccgcaagc aaattcataa
     3241 tacatgataa gaaaattgtg aatttttata ttgtaatggg aatctaggaa aaattcttat
     3301 ttacatttgt aagaaacttg tataattgaa gatttttctt tccttagttt tgttagtcat
     3361 agtggcttta cctgtttatg cacaggaaca gtctgaagag acagttttac caactgacaa
     3421 gggtaccatc aatgtaggac tgtctactat tccagatcaa ccaaatccaa gtcaagagac
     3481 aaagctcaag atcaattttt taaacaaaaa cacaaatatg attcaggaac atattgatta
     3541 cagcataact gtcaaaaaag gagaaagcca agtattttca attccgttaa ctcatactgc
     3601 aattggatca gttacaattc cataccaatt tgagatagct ggtcagtatc aagtctctgt
     3661 tgatatacaa ggtattctat ttcaaccaat tcctacagag tctgcagttt tttctatctc
     3721 tgttgttgat tctcaatcaa agaacggatg tttgatagca actgcagctt ttggttctga
     3781 gcttgctcca caagttcaat ttctaagaga atacagagac aacacagtaa tgacaactgt
     3841 tgcaggttct agttttctta atgtgtttaa tgcagtatac tattctttta gtccaacagt
     3901 tgcagatgtg gaaagaaaca atcctttcct acaggaatcc gtaagaattg gcataatgcc
     3961 acttcttgga atcttacaag tcacagagac tttgaacacc ggaggagaga tctcagttgt
     4021 aatatctggt atcattgcat cattactcgt tggtgcaata tatctatggc ctgctggatt
     4081 aattaccaga gcttatagga aatcattacg atcaagtacc aaatttgtga ttatcataat
     4141 ctctatagtc atagctttga cattgatttc aattgcaaca gcaaatgtac aatttatggc
     4201 aatttcaaca tcaatcatga ttttagcttt agctggtgct ggtgcgatat tttcatcaca
     4261 agcaatttgg aaagcggtac tgaaaataag aagcacattt ttataatcct cctttaaata
     4321 gcagacacat gaagtcaaaa tcaataggtt cgttttttgt tggtttgact cttgtagctg
     4381 cattagttgc aatagcacca agtgcttttg
//
LOCUS       DATIDL010000055        10295 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrosopumilaceae archaeon isolate SMAG_U16342
            k141_2287775, whole genome shotgun sequence.
ACCESSION   DATIDL010000055 DATIDL010000000
VERSION     DATIDL010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935836
            Sequence Read Archive: SRR25158326
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosopumilaceae archaeon (soil metagenome)
  ORGANISM  Nitrosopumilaceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrosopumilales;
            Nitrosopumilaceae.
REFERENCE   1  (bases 1 to 10295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/04/2024 05:09:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,520
            CDSs (total)                 :: 1,474
            Genes (coding)               :: 1,474
            CDSs (with protein)          :: 1,474
            Genes (RNA)                  :: 46
            rRNAs                        :: 1 (5S)
            complete rRNAs               :: 1 (5S)
            tRNAs                        :: 43
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10295
                     /organism="Nitrosopumilaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2287775"
                     /isolate="SMAG_U16342"
                     /isolation_source="soil"
                     /db_xref="taxon:2202732"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..182
                     /locus_tag="VLB45_02300"
     CDS             <1..182
                     /locus_tag="VLB45_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007401369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04566.1"
                     /translation="DEKGKLKLKGVSPKMLKRMKEEAVDCPVLKEEVGFIQCFVCSNF
                     QSRVTGKVLCKGDPL"
     gene            226..1668
                     /gene="proS"
                     /locus_tag="VLB45_02305"
     CDS             226..1668
                     /gene="proS"
                     /locus_tag="VLB45_02305"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HSD04567.1"
                     /translation="MSKEIGITVSKKENFSEWYTQVVIKAELADYAPVKGLIVLRPYG
                     YSIWESLKSSLDEKLKSTGHENGFLPVLIPESLLSKEASHFAGFNPEVFWVTHSGETE
                     IGDKLALRPTSETLAYSMYSKWIKSWRDLPLKINFWNTALRAEIKATKPFLRTSEFLW
                     QEGHTVHSTKEEAEQEVRTILEIYKKTVEEELAIPVITGKKSEKEKFVGAVYTTTMES
                     IMPDGKALQMGTSHFLGQNFSKPFDVKFLDKNNVENFAWQTSWGVSWRLIGALIMVHG
                     DDKGLVLPPRVAPIQVVIVPIYYSDEDKKIVLEKTIHIQQVLAKNKIRVHVDSRDQWT
                     PGYKFHDWEMKGVPLRIEIGPKDIAKNKMVLVRRDTQKKSDLEFSNIETGVESMLKEI
                     QQSLFEKARELLKEKTKNISDFEKFKSELEIGAFLNSSWCGDQKCEEEIKETTGADIR
                     VIPFDTTKSDSPCINCKRPGKENVIFARGY"
     gene            1709..2347
                     /locus_tag="VLB45_02310"
     CDS             1709..2347
                     /locus_tag="VLB45_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HSD04568.1"
                     /translation="MDLGDILPIDPEVGYLGLLLVSFFGSLIPFVPFPAFILLATMSV
                     GDKFDLHALVLISAITSTTAKQIIFYLSYGGRKMINEKTRKRIKPFERLVKRYGAAAA
                     FIAAATPIPDDLVYVPLGLARYNPLRFFISTLAGKVVLSYAIVLVAHGFGTSYIDPLL
                     DQVHDTSTVYIGIIIFVAMMTGVVVLMLRLNWARVLGKIAPWTLNPEKDENS"
     gene            complement(2344..2904)
                     /locus_tag="VLB45_02315"
     CDS             complement(2344..2904)
                     /locus_tag="VLB45_02315"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010190117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HSD04569.1"
                     /translation="MRTLSRSFYARDTVDVARDLLGKLLVRKIGKKMISGIIIETEAY
                     RYKDDQASHSFGGMTERNKVMFGQVGKAYVYFTYGMHYCVNAVARDDSYDAGAVLLRA
                     LEPKDGLEFMMKQRNTKEISNLTSGPAKLTQALQITKKQYGEDLTKLSELFITEGIEI
                     KKSDIVERPRIGIKKATRRLWNFTIS"
     gene            complement(2913..3479)
                     /locus_tag="VLB45_02320"
     CDS             complement(2913..3479)
                     /locus_tag="VLB45_02320"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HSD04570.1"
                     /translation="MPNIDEIKSQVISCVKCSLSKARTCAVPGKGNPKAELLFIGEAP
                     GRSEDLQGEPFVGAAGKILSEALSFVGLSRDEVYITNVVKCRPPNNRVPIQEERDACS
                     NYLAWELETIKPKIICIMGNTAYGSLLGGNSITKNRGKVIEKNGRLYFLTVHPAAAIY
                     NQELKSVLKNDMKMLVETLARLKNDDRT"
     gene            complement(3480..4898)
                     /locus_tag="VLB45_02325"
     CDS             complement(3480..4898)
                     /locus_tag="VLB45_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008299471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HSD04571.1"
                     /translation="MNLEDYADKAVEFALQNGSQYCDVRAETIESQGFLLEDGEIEHF
                     ITSNDSGLGIRVLAGGAWGFHSISNPTSLEEIKIAILGAIKSALHYSSRKTHKVKLAE
                     TRAYVDKINFNVEKLPVVDDLIKLGLECDSIIKNKKRISKSSISIEYDTFTKYFVNSD
                     GARINHNFVDTIANLSATAYESGLTESVNATEGGRGGMEMILGKNDITKTAEFVASKA
                     SELIDAKTIKEDKATIVMNPDFVALLTHEILGHPSEADRVLGKEMAWAGGAWWAGKLG
                     QQVGSDALTVIDDPTIAGNLGCYKYDDEGVLAKKKTLIENGTLVNHMQSRETASLFDT
                     EPNSGMRATKYEFMPLIRMACTCIKEGKWDPQEMIKEVKNGYLVSNMKVPSIDMMRYN
                     WSISCQFAQKIENGEPTELLRDVIVMGTAPDFFNSIDACGKDFCVRPITNCGKGDPMQ
                     IMRMGNGGPHIRGIATVKSVGT"
     gene            complement(4937..6811)
                     /locus_tag="VLB45_02330"
     CDS             complement(4937..6811)
                     /locus_tag="VLB45_02330"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HSD04572.1"
                     /translation="MKLIHKTYLLLIIVIAAAIVNLVLLLATARENENDLHALIHANN
                     LKVTTERVAGLANSIASGNEEARENLIARINEYDAAYGVLRTGGMIDEDQVTAVPSEL
                     KTDYEKIGLSWGIYKKNALTIQKESVFDPEIRNTLRYVLEKNGQVLTLTDEITQDLST
                     LDRNYNRHKEIALELTNIATSIGEKTLLMSIGEGDEHREDLKKNRLLFDANLKKLLQL
                     PLDNEEIDDLDIKPETLSPIPRENSDALRKLDPLWESLQPKLMVVETNSIISKEFGAA
                     LNALREERLTLLELTDDFANSWNDILDKKLNDRGLLIQSLLAADIAVILFVLRTIRSS
                     LNPLNRLAEALGRIKEGIYGEKIQHTSKDEIGVLTDTFNEMSMTIKEKEDEAKKIEIA
                     KDEFLAMITHELKTPLVPIQGYADILIRGHLGDLNKNQKERLEIIKSSSASLLALISD
                     LLDVQKLELGQLRIKKKPQSIKEIVAKALDVLTPQATTDQIKLVDGIKKDIIVTCDDD
                     RIRQVLTNLIKNSIKAAQPKIGLVEVLAEDSNDEVKISVKDNGKGIPKESQNKIFKKF
                     YQVDTSSTREKGGSGLGLSICKGIVETHGGRIWFESEPGKGATFTFTLPKDQQTKSAI
                     "
     gene            6880..7248
                     /locus_tag="VLB45_02335"
     CDS             6880..7248
                     /locus_tag="VLB45_02335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04573.1"
                     /translation="MSDDFLKLARKEIQGELDSLQEILSHCHNDADITRYGKDIEKHL
                     HKIKGLAPMMGQEGIGEIARLNDAIVRHIIANGNLVDTYETLSESIKIMKGVFENSNN
                     ANVEQIKSKLLQTFPNVLNQ"
     gene            7298..7663
                     /locus_tag="VLB45_02340"
     CDS             7298..7663
                     /locus_tag="VLB45_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010190110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSD04574.1"
                     /translation="MARIMVADDSDAIRMVLKDALEIGHHEVVAEATNGIEAVEKYNE
                     VNPDVLLLDIAMPKKDGASALKDIMATHMDAKVIMITASDNLKTINECVSIGALAYIM
                     KPFDFDDVLKAIAMALDKR"
     gene            complement(7667..8593)
                     /locus_tag="VLB45_02345"
     CDS             complement(7667..8593)
                     /locus_tag="VLB45_02345"
                     /inference="COORDINATES: protein motif:HMM:NF037270.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSD04575.1"
                     /translation="MSSQKKNILIIEDSLAVASLMQDFLKKLDYQEIYISDTGRNGIQ
                     VFENLVKSNKTPVVILDYRLPDMNADEIMTEIFKIKPNTKIIIESANEKTDESIKDVL
                     RSGAYEYLEKPIRFENLKNTMRVLEDEDKILVNKSPESQTSQTQINSLLNSSVQISLA
                     RISEYCHMSKEETIEHLKKLESDGKIIKLDDIKEISCNLCNSMKIGQTFHCPSCGNSN
                     FKQGTLIEHYKCGNFSSDDSYKDNICPKCRKEIKVLGVDYKLLKNYYICDSCGNKFPD
                     PTQEYLCLKCNNNFKLEQAKWIVSSGFRAVNL"
     gene            8670..>10295
                     /locus_tag="VLB45_02350"
     CDS             8670..>10295
                     /locus_tag="VLB45_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PINc/VapC family ATPase"
                     /protein_id="HSD04576.1"
                     /translation="MDKIVADTSIIIDGEIGKLIESGNIKNCEIIIPMAVLDELQSQA
                     SQSKEYGFVGLEEVKKIRQISEKHGITIRFEGERPSMDDIKLASHGRIDAIIKDIAKK
                     NDAIFYTADYVQALVAEAEGVKTRYSKPDVKTTNLEFEKFFDSQTMSVHLKEGTKPMA
                     KRGKPGAFTLLELEEKILTKDYLTSITTEILEASRINQSGIVEISKPGALVIQYKDFR
                     IAITHPPFSDNYETTIVHPTVRLSLEQYKIPEKLMTRFSEKAEGILVSGPPGSGKSTF
                     ASSLADFYSQKGKIVKTLESPRDLQVGDKVTQYTQLDGSFENSADILLLVRPDYTIFD
                     EVRRFTDFRVFADLRLAGVGMVGVVHANAPLDAIQRFIGKVELGMIPHILDTVIFIKN
                     GDIAQVYELEFKVKVPTGMTEQDLARPVIEVRNFENRNLEYEIYTYGEENIVIPISKE
                     AKSGGIEKLAESKIRETIRRFDPDAEIEILSNSRIQVRVDKEVMPSIIGRGGSTINEL
                     EKTLGVHIDVEPKVHTFGNEIDFELSEAGNNIVL"
BASE COUNT         3160 a         1898 c         1898 g         3339 t
ORIGIN      
        1 tggatgaaaa gggcaagctg aaattgaaag gtgtcagccc aaaaatgcta aaacgaatga
       61 aagaggaagc cgttgattgc cctgtgctta aagaagaagt aggtttcata cagtgttttg
      121 tgtgttctaa ttttcaaagc agagttacag gaaaagtgct ttgtaaaggc gacccactct
      181 aaaaccagaa aggctcatta gtatagtttt tcaaaatgat gtgatttgag caaagagatt
      241 ggcattacag tttcaaaaaa agaaaatttt agcgagtggt acacccaagt tgtaataaaa
      301 gctgagcttg cagactatgc tccagtaaaa gggctcattg ttctcagacc atacggatat
      361 tccatttggg aatccctaaa gtcatcactt gatgagaagt taaaaagtac tggtcatgaa
      421 aacggttttt tgccagtact gattccagaa tcacttctca gtaaagaagc tagtcatttt
      481 gcaggattca atccagaagt tttctgggtc acacattctg gcgagacgga gataggagac
      541 aagcttgcac tacgcccaac atctgagacc ttggcatatt caatgtattc caaatggata
      601 aagagttgga gggatctgcc tctgaagatc aatttctgga atactgccct gcgagcggag
      661 atcaaggcta caaaaccatt tcttaggaca tcagaatttt tgtggcaaga aggacatacg
      721 gttcactcta caaaagagga agcagaacaa gaagtcagaa caatactaga gatttacaaa
      781 aaaactgtag aagaagagct tgccatacca gtaattacag gtaaaaaaag tgaaaaagaa
      841 aaatttgtag gtgcagttta caccacaaca atggaatcca taatgccaga tggaaaggca
      901 ttacaaatgg gaacatcaca ctttttgggc cagaattttt caaaaccatt tgatgtaaaa
      961 ttcttggaca aaaataacgt agaaaatttt gcgtggcaga catcgtgggg ggtttcttgg
     1021 agattaatcg gtgctttaat aatggttcat ggagacgaca aggggctggt gcttcctcca
     1081 cgagttgctc caattcaagt agtcattgtt ccgatttatt attctgatga agataagaaa
     1141 atcgtactag aaaaaactat ccatatacaa caagtactag caaaaaacaa gataagggtt
     1201 catgtggatt ctagagacca gtggactcca ggctacaaat ttcatgattg ggagatgaaa
     1261 ggagttccat tacgaataga aattggtcca aaagacatag ccaagaacaa gatggttcta
     1321 gtcagacgag acacacagaa aaaatcagat ctagaatttt ccaacattga gacaggagta
     1381 gaatcaatgc taaaagagat tcaacagagt ttgtttgaaa aggcaagaga attactcaaa
     1441 gaaaagacaa agaatatttc tgattttgaa aaattcaaat ctgaattaga aattggcgca
     1501 tttttgaatt catcttggtg cggtgatcaa aaatgtgagg aggagattaa agagactact
     1561 ggggcagaca tacgagtaat tccttttgac actacaaaat cagattctcc ttgcatcaat
     1621 tgcaaaagac cgggcaaaga gaacgtgatt tttgcacgcg gctactaaac aaataactga
     1681 ttaaatatag gctagccagt ggttgactgt ggatttgggc gatattttac caatagatcc
     1741 tgaagtgggc tatctcggac ttctacttgt aagctttttt ggttcgctga ttccgtttgt
     1801 cccctttcct gcattcatac tgttagcaac aatgtctgtg ggagacaaat ttgatcttca
     1861 tgccctggta ctaattagcg caattacatc aactacagca aagcagataa ttttctatct
     1921 tagctatggt ggaagaaaaa tgattaatga aaaaacacgc aagagaataa aaccgtttga
     1981 gagattggtt aaacgttatg gtgcagccgc tgcctttata gctgcagcaa ctccgatccc
     2041 agatgatttg gtttatgttc ccttgggact tgcaagatac aatccattga gatttttcat
     2101 ttctacactt gcaggaaaag ttgttcttag ttatgccatt gttcttgttg cccacggatt
     2161 tggtacctcc tacatagacc cattacttga ccaagtacac gacacgagta cagtctacat
     2221 aggcataatc atttttgttg ccatgatgac aggtgttgta gtattaatgc tacgtctcaa
     2281 ctgggcaaga gtgctaggca agattgctcc atggacatta aatcctgaaa aagacgaaaa
     2341 cagttaggat atagtaaaat tccaaagacg tctagttgct ttttttattc caatccttgg
     2401 cctttctaca atgtcagatt ttttaatttc aattccctcc gtgataaaaa gttccgacag
     2461 ttttgtgaga tcctcaccat attgtttttt agtaatttgt aatgcttgtg tcagctttgc
     2521 aggtccactt gtaagatttg aaatttcttt agtatttctt tgcttcatca tgaattccaa
     2581 accatccttt ggttctagtg cacgcaagag tacagcacct gcatcataac tatcgtctct
     2641 agcaacagca ttaacacaat aatgcattcc ataagtaaag tagacatacg cttttccgac
     2701 ttggccaaac atcaccttgt ttcgttctgt catgccgcca aaagagtgac ttgcctgatc
     2761 atctttgtat cgatatgctt ccgtttctat tattattcca gagatcatct ttttacctat
     2821 ttttctaaca agcaattttc ctagtaaatc tcttgcaaca tcaactgtat cccttgcata
     2881 aaacgatcgt gaaagtgttc tcatgaaata ttctaagttc gatcatcatt ttttaatcta
     2941 gctagtgttt cgaccaacat cttcatgtca ttttttagta cagattttag ttcttgatta
     3001 tatattgctg cagcaggatg cactgttaaa aaatacaagc gcccattttt ttcaattacc
     3061 ttaccacggt tctttgttat cgaattacca ccaagtagtg atccatatgc agtgttcccc
     3121 ataatgcaga ttattttggg ttttattgtc tcaagttccc atgcaagata gttactacag
     3181 gcatcccttt cttcctgaat aggcactctg ttatttggag gtctacactt tactacattt
     3241 gttatataga cttcatctct tgagagacca acaaaagata atgcctctga taagatcttt
     3301 ccagctgcgc caacaaatgg ttcgccttgc agatcttcgc ttctacctgg agcttcgcct
     3361 atgaataaga gttctgcttt tggattgcct ttgcctggaa ctgcacatgt tcttgctttt
     3421 gaaagactgc atttgacaca tgaaattacc tgcgatttta tttcatctat atttggcatt
     3481 tatgttccaa cacttttgac tgtggcaatt ccacgtatat gaggtcctcc atttcccata
     3541 cgcattatct gcataggatc tccttttccg cagtttgtga ttggtctaac gcaaaaatct
     3601 tttccacatg catcaataga attgaaaaaa tctggagcgg ttcccatcac tatgacgtct
     3661 cttaacaact ctgttggctc accattttct atcttttgtg caaactgaca cgatatactc
     3721 cagttgtatc gcatcatgtc tatcgatggt actttcatat ttgaaacaag ataaccattt
     3781 tttacttctt ttatcatttc ttgtggatcc catttacctt ctttgatgca tgtacatgcc
     3841 attctaatta gcggcataaa ctcgtatttt gtagctcgca tgccagaatt tggttccgtg
     3901 tcaaaaagag atgcggtttc tctgctttgc atatgattta ccagagtgcc attttctatc
     3961 aaagttttct tttttgcaag aaccccttca tcatcatatt tgtaacatcc tagatttcct
     4021 gcaattgttg gatcatcaat aactgtcaat gcgtcagagc ctacttgttg tccaagttta
     4081 ccagcccacc aagcacctcc agcccaagcc atctccttcc caagaactct gtctgcctct
     4141 gatggatgac caagaatctc atgtgtgaga agggcaacaa agtctggatt catcactatc
     4201 gtagccttgt cttctttgat agtttttgca tcaataagct cagatgcttt gcttgctaca
     4261 aattctgcag tctttgtgat atcgtttttt ccaagtatca tttccattcc acctctgcca
     4321 ccttctgtag cattcactga ttcggttaga ccagattcat atgcagttgc agaaagattt
     4381 gcgatggtgt caacaaagtt atgatttatt cttgcaccgt cactatttac aaaatatttt
     4441 gtaaatgtat catattctat tgatattgat gatttgctta ttcttttttt gtttttgatt
     4501 atactatcgc actctaatcc tagtttaatc agatcatcaa ccacaggtag cttttctaca
     4561 ttaaaattaa ttttatctac atatgcacga gtctctgcaa gttttacttt atgtgtcttc
     4621 cttgatgaat agtgtaatgc actttttatt gccccaagga tagcaatttt gatttcttct
     4681 aatgaggtcg gattcgaaat agaatgaaac ccccatgcac ctccagcgag aactctaatg
     4741 ccaagaccag aatcatttga agtgatgaaa tgttcaattt ctccatcttc taaaagaaat
     4801 ccttggcttt ctatcgtttc tgctctgaca tcacaatatt gtgaaccatt ttgtaatgca
     4861 aattctactg ctttatcagc ataatcttct agattcaaca atgtgttcaa tagacgtagt
     4921 gtattttttg tttgtgttat attgcagatt ttgtttgttg atctttagga agagtgaatg
     4981 taaaagtggc acctttaccg ggctcacttt caaaccagat cctaccgcca tgagtctcaa
     5041 caataccttt acagatggat aatcctaatc cacttccacc tttttcccgt gttgacgatg
     5101 tatctacctg atagaatttt ttaaatattt tattctgtga ttctttggga atgccttttc
     5161 cattatcttt aacagagatc ttcacttcgt cgttggaatc ctccgccaga acctccacta
     5221 gaccaatttt tggttgcgca gctttgatac tattttttat caagttagtc aatacttggc
     5281 gtatacggtc atcatcacat gttacaataa tatctttttt tatgccatct actaatttga
     5341 tttgatctgt tgtagcttgc ggagtcaata catctagggc ttttgcaaca atctctttga
     5401 tgctttgtgg tttcttttta attcgaagtt gtcccagctc taatttttgt acatcaagca
     5461 gatcagatat gagtgccaaa agtgatgcag agcttgattt gattatttct aatctttctt
     5521 tttgattctt gttaagatct cctaaatgcc ccctaatcaa aatatcggca tatccctgta
     5581 taggaactag tggcgttttt agctcatgcg ttatcattgc aagaaactca tcttttgcta
     5641 tttcaatttt ctttgcttca tcttcttttt cttttatcgt catagacatt tcgttgaacg
     5701 tgtcagtcag cacaccaatc tcatcttttg aagtatgttg aattttttca ccgtaaatcc
     5761 cttcctttat acgaccaagt gcttcagcta gtcggtttaa tgggtttaat gagcttctaa
     5821 ttgtacggag aacaaaaaga attactgcaa tatctgctgc caataatgat tgtatcagta
     5881 atcctctatc attgagtttt ttatctaaaa tatcattcca agaattagca aagtcatcag
     5941 tcaactccaa aagagtgaga cgttcttccc tgagagcatt tagtgcagcc ccaaattctt
     6001 ttgaaattat tgaattagtt tctactacca ttagttttgg ttgtaatgat tcccataaag
     6061 gatccaactt tcttaatgca tcagagtttt ctcgtggaat cggtgaaagc gtttctggtt
     6121 ttatatcaag gtcatcaatt tcctcgttgt ctaatggaag ttgcaataat tttttcagat
     6181 tagcatcaaa aagcaatcta ttttttttca gatcctcacg atgttcatcg ccttcaccaa
     6241 ttgacattaa aagcgtcttt tctcctatag atgttgcaat gtttgttaat tccaatgcaa
     6301 tttctttatg tctgttgtaa ttacgatcaa gtgtggaaag atcttgcgtt atttcatcag
     6361 tcagtgtaag tacttgtccg tttttttcta gaacgtatcg tagtgtattt ctaatctcgg
     6421 gatcaaagac cgattctttt tgtattgtta atgcattttt tttgtatatc ccccaagaaa
     6481 gtccaatttt ttcataatca gtttttaact cagaaggcac tgcggtaact tgatcttcat
     6541 ctatcatacc accagtacgc aatactccgt atgcagcatc atattcatta attctcgcaa
     6601 tcaggttttc tctcgcttct tcattgccac ttgctataga atttgcaaga cctgcaactc
     6661 gttctgttgt gactttgaga ttatttgcat gaataagagc gtgtaaatcg ttctcatttt
     6721 ctcttgctgt tgctaaaagt aaaacaagat tgactattgc agctgcaatt acaataatca
     6781 ataacagata tgttttgtga atgagcttca ttggcgctta aatttgtaaa ttaagagcct
     6841 ataaaagtta gtattaaagt tatctacttt gtagttaaaa tgtcggatga ttttctaaaa
     6901 ttggctagga aagagattca aggtgaactg gattctctgc aagagatttt atcacattgt
     6961 cataatgatg cagacattac cagatatggg aaagatatcg aaaaacatct tcataagata
     7021 aaaggtcttg ccccaatgat gggacaggaa ggaataggag aaattgcaag attaaacgat
     7081 gccatagtaa gacatatcat tgcaaatgga aatcttgttg acacgtatga aaccctgtca
     7141 gaatccatta aaatcatgaa gggtgttttt gagaattcaa ataatgctaa tgtggaacaa
     7201 atcaaaagca aattgttaca aacttttccc aacgtactca atcagtgata cagatctgag
     7261 cttaaatatg cagtatgtat gattagattt ctgaaacatg gcacgtataa tggtcgcgga
     7321 tgattctgat gcaatcagaa tggttctcaa agatgcgctg gaaattggcc atcatgaagt
     7381 tgtggcagaa gctactaatg gaatagaagc tgtagagaaa tataatgaag taaatcccga
     7441 tgtattgcta ttggatatag caatgccaaa aaaagatggg gcatctgctc tcaaagacat
     7501 aatggcaaca catatggatg ccaaagtcat aatgataaca gctagtgaca atcttaaaac
     7561 aattaacgaa tgtgtttcaa taggagcact agcttacata atgaaaccgt ttgattttga
     7621 tgatgtgcta aaggctattg caatggcact tgacaaacgc taagaatcac agattaacag
     7681 ctctaaaacc actactcaca atccattttg cctgttcaag cttgaagtta ttgttgcatt
     7741 tcaagcacag atattcttga gttggatctg ggaatttatt tccacaacta tcacagatat
     7801 aataattttt taggagttta taatcaactc caagaacttt gatttcttta cgacattttg
     7861 gacagatgtt gtccttatac gaatcgtcag aagaaaaatt accacatttg tagtgttcaa
     7921 tcaacgtacc ttgcttgaaa tttgaattac cacaggaagg acaatgaaaa gtttgtccta
     7981 ttttcatcga gttacataaa ttgcatgaaa tttctttaat atcatctaat ttgataattt
     8041 ttccatctga ttctaatttc ttcaggtgtt caatagtctc ttctttggac atgtgacaat
     8101 attcagagat tcttgccaga ctaatctgca ctgatgaatt tagcagagaa ttaatttgtg
     8161 tttgcgatgt ttgtgattca ggagatttgt ttacaagaat tttgtcttca tcctcaagta
     8221 cccgcattgt gttttttaga ttttcaaacc taattggttt ttcaagatat tcgtatgcgc
     8281 cagatcgtag aacgtctttt attgattcat ctgttttttc atttgcactc tcaattatta
     8341 tttttgtgtt aggtttaatt ttaaatattt cagtcattat ttcatctgca ttcatgtctg
     8401 gtagcctgta atcaagtatg accacaggag ttttgtttga ttttacaaga ttctcaaaaa
     8461 cttggattcc atttctaccc gtgtcagaaa tgtagatctc ttgatagtcg agttttttta
     8521 gaaaatcctg catcagggat gctacagcaa gactgtcttc tattatcaat atattcttct
     8581 tttgtgaaga catgccaaat attcaggatt gagtgaatat agatttttac aggtatgaat
     8641 aaaaagcgcg agataacgct tggtatgtaa tggataagat tgttgcagac actagcataa
     8701 taatagatgg tgagattgga aaactaatcg agtctggcaa tatcaaaaat tgtgagataa
     8761 ttatcccgat ggctgtgctt gatgagcttc aatcacaagc ttcacaaagt aaagaatatg
     8821 gttttgtggg gctggaagaa gtcaaaaaaa ttcgtcagat ttctgaaaaa catggtataa
     8881 caataaggtt tgagggagag cggccaagca tggatgacat caagttggca agccatggtc
     8941 gtattgatgc aataataaaa gatattgcaa agaaaaatga tgcaattttt tacactgcag
     9001 attatgtaca agcactagtt gctgaagccg aaggagtaaa gacaagatat tcaaagcctg
     9061 acgtcaagac cacaaatcta gaatttgaga aattctttga ttcacaaaca atgagtgttc
     9121 acctaaaaga aggcacaaaa cccatggcaa aacgtggaaa acctggtgca ttcacactct
     9181 tagaattaga agaaaaaatc cttacaaagg attatctaac ttcgataact acagaaattt
     9241 tggaagcatc aagaattaac caatctggaa ttgtcgagat ttcaaagcct ggtgcattag
     9301 ttatacaata caaagatttt cgcatagcaa taactcatcc tccattttca gacaattatg
     9361 aaaccaccat agtacacccc actgtaagac tttcattgga gcaatacaag ataccagaaa
     9421 aactgatgac aagattttcg gaaaaagcag aaggcattct tgtatcaggg ccacctggat
     9481 caggaaaaag cacttttgca tcaagccttg cagattttta ttcacagaaa ggaaagattg
     9541 tcaaaacact ggaatcacca agagatctac aagtaggaga caaggttaca cagtataccc
     9601 agcttgatgg aagttttgaa aattctgctg acattttact tttggtaaga ccagattata
     9661 ccatctttga tgaagttcga aggtttactg attttcgagt ctttgcagat ttgaggttgg
     9721 caggtgttgg catggtgggc gtagttcatg caaatgcgcc tcttgatgca attcagagat
     9781 tcataggaaa agttgagctt ggaatgattc ctcacatttt ggatacggtc atatttataa
     9841 aaaatggaga cattgcccaa gtttatgaat tagagtttaa agtgaaagtt ccaacaggca
     9901 tgacagagca agatcttgca agacctgtaa tcgaggtacg caattttgag aaccgaaact
     9961 tggaatatga aatttacact tatggcgagg aaaacattgt aatcccaatc tcaaaagagg
    10021 caaaaagcgg cggaatagaa aagcttgcag aatccaagat aagggaaaca atacgcaggt
    10081 ttgatccaga cgcagaaatt gagattttat caaacagccg cattcaggta cgagtagaca
    10141 aggaagtaat gccatctatc attgggcgtg gcggttctac aataaacgag cttgaaaaaa
    10201 cacttggtgt tcatatcgat gtggagccaa aggtccacac atttggaaac gagattgatt
    10261 ttgagctttc tgaagctgga aataatatag tgctt
//
LOCUS       DATIDL010000056        25872 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrosopumilaceae archaeon isolate SMAG_U16342
            k141_2693386, whole genome shotgun sequence.
ACCESSION   DATIDL010000056 DATIDL010000000
VERSION     DATIDL010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935836
            Sequence Read Archive: SRR25158326
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosopumilaceae archaeon (soil metagenome)
  ORGANISM  Nitrosopumilaceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrosopumilales;
            Nitrosopumilaceae.
REFERENCE   1  (bases 1 to 25872)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25872)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/04/2024 05:09:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,520
            CDSs (total)                 :: 1,474
            Genes (coding)               :: 1,474
            CDSs (with protein)          :: 1,474
            Genes (RNA)                  :: 46
            rRNAs                        :: 1 (5S)
            complete rRNAs               :: 1 (5S)
            tRNAs                        :: 43
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25872
                     /organism="Nitrosopumilaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2693386"
                     /isolate="SMAG_U16342"
                     /isolation_source="soil"
                     /db_xref="taxon:2202732"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1088
                     /locus_tag="VLB45_02355"
     CDS             <1..1088
                     /locus_tag="VLB45_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HSD04577.1"
                     /translation="IVEEVEKALDIAHQIGYPVLLKSVFGGGGRGIRLVHNEKELREA
                     FEIASGESKAAFGKSALFVEKFLPKIRHIEYQLFRDKHGNAVHIFERECSIQRRHQKL
                     IEMSPSPVVDQKTRDEIGKLAINAATAVDYTNAGTAEFLRLDDGTFYFIEINARLQVE
                     HPVTELVSGLDLVKLQIDIANGKEIPFKQKDLKLKGCAIECRINAEDTFLDFAPSTGP
                     IPDVNIPSGPGVRVDTYLYPGCTVSPYYDSLMAKLVTWGGTFEESRQRMQLALSDFYI
                     EGVETSIPLYKTILDSEEFINGDLSTDFLDRFKILDRLRDDIKKNISYNTDAALAAAV
                     IHSEFLKNRIKSRVENNIRWKSQLDSA"
     gene            1093..1605
                     /locus_tag="VLB45_02360"
     CDS             1093..1605
                     /locus_tag="VLB45_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007549632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HSD04578.1"
                     /translation="MDFRIENLGSKFDAEIIRSLGNSEYVVKIQGKERNLKILNMTSD
                     KIEFMLDNTHHTVKYLDNNTAKMTLVVNGVQITFDKHTSLDEIVYKNSGSESGGDSQL
                     SLRSQIPGRVVSINTTVGNQVKKGDVLCVLESMKMQVSIKSHKDGVIKSMKIKQGASV
                     AKNDVLMEIE"
     gene            complement(1632..2105)
                     /locus_tag="VLB45_02365"
     CDS             complement(1632..2105)
                     /locus_tag="VLB45_02365"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007549633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HSD04579.1"
                     /translation="MTVNVGDKAPSFTLVDTDLKMRSLDEFKGKKVVLSFFVAASSPV
                     CTKEMCTFRDSWEQISKLGAQVIGISNDGPFANKAFAETHHLNFPVLGDYKSQTIRDY
                     DVLMPDLLHVKGYDAAKRSVFIVDEGGKIVYKWVSDNPLIEPNYQEIMNFLNKGN"
     gene            2189..2332
                     /locus_tag="VLB45_02370"
     CDS             2189..2332
                     /locus_tag="VLB45_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007550213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04580.1"
                     /translation="MPSMKCVSCGIGFFSPTGSDKCSRCVEKGSHGTEESHDSCGCGH
                     SHH"
     gene            2509..2841
                     /locus_tag="VLB45_02375"
     CDS             2509..2841
                     /locus_tag="VLB45_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HSD04581.1"
                     /translation="MFGFAIVATAPALILSRMISPRKRSNPVKFLPMECGQVPSGEGR
                     THFMMQYYAYVLMFVVFDVMSIFLYAWGTTLLNLPKSATLPIIAFLGIMFASFAFALY
                     QSGRRNIW"
     gene            2888..3403
                     /gene="nuoB"
                     /locus_tag="VLB45_02380"
     CDS             2888..3403
                     /gene="nuoB"
                     /locus_tag="VLB45_02380"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HSD04582.1"
                     /translation="MQKELISQNAANVFVGKLGDILVKAIDKPLGYAINWGRLWSLWP
                     VHIETACCSVEFGAASGPRYDVERFGIIEAFGSLRQCDLVVVQGTITRKMAPRLRMVY
                     DQMPEPKYVIAMGACAITGGLYFDSYNVLPGIDGILPVDVYVPGCPPRPETLIQGCML
                     LQEKIKRMKAR"
     gene            3406..3987
                     /locus_tag="VLB45_02385"
     CDS             3406..3987
                     /locus_tag="VLB45_02385"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HSD04583.1"
                     /translation="MSSETTDVKPSETVGTKPDVAKKETVSIAEPTFEKSLAEKITSK
                     FGTKSVIGFIKPNRIRINTKKEDILNIAAYIRDELNFDHAESVTGVDYPNSKEIEVVY
                     HLGSYSDPQLAKQILTLATRLPREEIPNPGNDSTRMPSLRDIFYSVEFHERECFEMLG
                     VYFENHPDNRRLLLPEDWADIPPMRKDFKIKGR"
     gene            3989..5122
                     /locus_tag="VLB45_02390"
     CDS             3989..5122
                     /locus_tag="VLB45_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HSD04584.1"
                     /translation="MTVRLPPGIQLEKADERIMTLNVGPQHPGSGHMRLIIKVDGDYI
                     VSCDPDPGYVHRGEEKMAEYRNYIQNIPHLERPVIHDSCNILYPYCLAVEDLLGIEVP
                     ERAKYIRVIASELNRCVYTLYWLAIYGIFLGHSTMFMWPAGDRELFIDLLEAMSGARV
                     THAYFVPGGVRNDLPANFEERCLKQVAYFEHRLKQYEDIFYQNPVLKQRTEGTGILSR
                     MDAIRLGTTGSVLRSAGVDFDIRKKEPYDVYENLDFQSQVLKEGDSYARSRVPYLDML
                     ESCRIIKDALKKMPKSGSVRVKLKPNPKGGNEEVYRRVESGRGSLGMYVVSDGKPEPY
                     RVKISVGSFRNLICMPYLLKGEKLGNMPAVYWSLNYWPVEADR"
     gene            5123..6424
                     /gene="nuoH"
                     /locus_tag="VLB45_02395"
     CDS             5123..6424
                     /gene="nuoH"
                     /locus_tag="VLB45_02395"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007549639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HSD04585.1"
                     /translation="MSTIAPEFRLSRFIASLTDLIFWIVVLFTLIGLPAAMIALFYIE
                     LPEINGTLLTPYLALTWIADPSRTLPIIKSLMQTDFFRITTFPGFGFAALLAAATIFI
                     ERKFLAKMQLRVGPLYCGKIEGILQLMGDGLKLISKEIIIPAKADKPIFWAAPLLFVG
                     TAAAFISLIPVAPGWVVADIDVGLLAVFAVIGFFPIITVLSSWASNSKFPFIGGIRAL
                     HQMISFEIPLILSLLGIVILTGTLNLTEIVESQQHYWWIIFMPIGAIVFFTTILAELE
                     RIPFDLPEAESEIVAGWLTEFSGMMYGLVQLGSYLKLYAFAALFTILFLGGWNGPMVW
                     PPFPEEIITHGITMGPITAQIPGLPLLDQEMLNATLWFTIKTVGVILVILLPRGVMPR
                     IRIDLLLHTGWYKLIGLAFVNIFIALALYYAGVLGPGGLIQ"
     gene            6421..6918
                     /locus_tag="VLB45_02400"
     CDS             6421..6918
                     /locus_tag="VLB45_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008301096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit I"
                     /protein_id="HSD04586.1"
                     /translation="MTSAGGIIRALNSGVKHLAVKRFTLRYPEQKLKFVGDGFQFDPQ
                     TGVGIAGLKGRHILFHDKCTGCQLCAIACEGIAEAIAMVKVDETWKHNKKAIMPQIDF
                     GKCVFCGLCVDACPFYALYMTNDYELSSFTKEGLIYTPGQLAVIPKVDQDVEIKIDDR
                     GATHG"
     gene            6911..7423
                     /locus_tag="VLB45_02405"
     CDS             6911..7423
                     /locus_tag="VLB45_02405"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007549641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HSD04587.1"
                     /translation="MADAVFLAISVLTIGSAIAALEIRSMIYGSIALMVTLAGVAGFF
                     LLLDAPFVAMFQISVYVGSIAVLILFTVMLVRRELIFNKVEDPRRRYAGIGLMIVIML
                     GIGTIIIGSGIKSMEVDAPAVDFREIGEDFLTYYSPALVVMALILASAVIGALALARR
                     EDVANEQRTD"
     gene            7404..7709
                     /gene="nuoK"
                     /locus_tag="VLB45_02410"
     CDS             7404..7709
                     /gene="nuoK"
                     /locus_tag="VLB45_02410"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HSD04588.1"
                     /translation="MSNELIEFVLVSVALLAIGVYGLAVKRNAIRMLFAIEMIVNAAN
                     LNLVAFSRFIPNSQGQTLALFSIAIAAAEVAVGLALVIVAYRMYKNIDIADFRSLKG"
     gene            7709..9271
                     /locus_tag="VLB45_02415"
     CDS             7709..9271
                     /locus_tag="VLB45_02415"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008301088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HSD04589.1"
                     /translation="MQYLLLQAVFLPLLLSPVAYYLGRKSGPNAATWFTFGVLLYCTT
                     ILIAPVFSGTYEEHYPWTQMFGEFGFLLDGLASPFAIIIYVLSTVLALYSKPYMVAKI
                     KEQFEEKMHHQKQMIGDGQSTAMIESSALKGYLNNQIGIYFALYLVFAMGMLGTVLAT
                     NLIEFYVFFEVMLIPGFFLIAFYGYGARRRIALLFFFWTHVGAVVLLLGFLAIGLSIG
                     SFDFVDIREEGVPPDVLAFAAVAIVIGLAVKLAAFIFHIWLPYAHAEAPTPISALLSP
                     AMIGIGAYGLFRLLVMLLPGQYENLALYLNLWGLATMLYGGAMALMQDDIKRLFAYSS
                     ISQMGYLLFGIGSASALGLAGSEMMYVSHGLGKGLLFMMAGAIILQVGTRSMTKLGGL
                     AGKMPITAVCAMIGALTIMGVPPTSGFMGEWTLFYGALQTAIQEGSTIRMIAFGLGLV
                     STVITMSYILWMMKRIFFGKLPENLSNTKEANWYITAPMMVLAGFSIVVGIYPDIFFN
                     QIIPFMNGVMGI"
     gene            9275..11359
                     /locus_tag="VLB45_02420"
     CDS             9275..11359
                     /locus_tag="VLB45_02420"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008301127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HSD04590.1"
                     /translation="MASEILGFGLEAGGMSAWAIWILPFIAALIIPGVAKFSKRATSG
                     IAVGFALMSALSATTLLPAALEGYETHDQISWISSIGIKAGVLADPLAVLMANVVAWI
                     SFLIMVYSTGYMKGDRNITRFWFWMMFFIGSMQLIVLSDNFLQLFFGWEGVGLASYAL
                     ISFWYQDKKKDHVGTEGYTALGVLQYYSPTHAGMKAFIMTKVGDIMMLAGMFLIFAFA
                     GTFGFRELTNDTAWATTMSAQGLLIPAAVLLFGGAVGKSAQFPLNEWLLEAMTGPTAV
                     SALIHAATMVKAGVFLVARLGPLFFALAAVGVGMDQFFEIVTWVGAITALLLATQGMV
                     SPEIKKVLAYSTGSQIGYMMMALGIAGLSQQFVDGYTAGFFHLISHAMFKASLFMAAG
                     SLLHVVGSRFMTDMGGLRKHMRKTYAFMWAAGLALMGAPFITTGFWSKDAIFAAVYES
                     GNTWAIPIFAIALVTAIITAFYTTRMIGMVFFGNKSKHIEKMEKEGHHIHEAPKSMWI
                     PYGILAVLTIGIGIIGLSAEQGIHHLFTEYLSHTFGIESAHEEVALESEAMLGGFLSG
                     INPIALVASLLAFAVGFGLGYIFYIGRFVDPVKFVNSNLFFYGIHKFFLNRWYLNSII
                     YWCFVIAPLYFARGIWRYMESTVIDGMNKGSEKAVGWSAKVIQGTQTGISQSYLYVFG
                     AGILFVVLLLLI"
     gene            11370..12857
                     /locus_tag="VLB45_02425"
     CDS             11370..12857
                     /locus_tag="VLB45_02425"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HSD04591.1"
                     /translation="MIDFTSTPIILTVILGAIGVILPVINVIRKDKGSSSFYGAITFG
                     ALLAAIGFVIYQIISNHVIPAAIFSENVLVDDMFGAFFAIAMLIVAIMTTVGSFNYMR
                     GKAHPAVYYSLILLSSIGMVLIAYSTDLVMLLVAWELMSIPTYVLAGFMKKDPSSNEA
                     ALKYFLFGALSSAIIIYGISIAYGLTGSTNISEVISGFSNLQSDMMPLALLAVAMFIA
                     GFGFKMGLVPFHMWLPDTYEGSPTTISALLAAGTKKAGFAAALRVVIMGTVALNMEWT
                     FALGVVAIMTMTVGNLAAIMQKNITRMLAYSSIGQAGYILIGMSIAPFSQIGLQASLY
                     HILNHAVMKGAAFIAVAGIIATLAVSHLDKIKGLGKRMPITSIGLVISLLALAGVPPL
                     NGFWSKLMLFGSAIDAGSVVSWAPYLAIAGVLNSALSLAYYGWIIRKMYFEEGESEKR
                     VKEPKSIIAVMIFSIIFMVATGVYPDPMIQFAQSAVPNLTMPTVP"
     gene            complement(12844..13698)
                     /locus_tag="VLB45_02430"
     CDS             complement(12844..13698)
                     /locus_tag="VLB45_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HSD04592.1"
                     /translation="MDRKNFEINPLIKDYKQYLDKINLALKHELELYSASEFVEPLQY
                     ALDGGKRIRPLILMLSAESVGICDENAYTAACAVELLHTESVIHDDIIDNETLRRRKD
                     PFHIKYGYNTSILTGDFVLGLILNITSRIDNPRIASELATTAMMMSDGEMIEARLESS
                     EDVTFDDYLKVIEYKTATAFEAASKIGAILGGGTEEQILALAEYGKNVGIAYQIHDDL
                     QDWNNEDKLFNVLIKKSSDPRDVFNHMDYLLKDYSLKSKSGLRKIEECTAKSHLENLV
                     DLTMFKVQ"
     gene            complement(13751..13842)
                     /locus_tag="VLB45_02435"
     tRNA            complement(join(13751..13764,13781..13842))
                     /locus_tag="VLB45_02435"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13804..13806),aa:Glu,seq:ttc)
     gene            13925..14563
                     /locus_tag="VLB45_02440"
     CDS             13925..14563
                     /locus_tag="VLB45_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HSD04593.1"
                     /translation="MNSLVKRIDELVEKQSLLKHQFYVMWSEGKLTLNSLNGYSKEYF
                     QLVKAVPSFVGAVMQQSPNSIKNELAANQKEETEHITPWMKFASALGVSQTELEKYSG
                     LVKTRKAVAELASLMNSYEDGAAAMYALEQEIPKISLSKIDGLRKFYGLTSDEAIEYF
                     RLHAEADIRHAATWRRVLENVSDEKEEELFQTASRSIAAQNMLLDSCYEAYC"
     gene            14608..14681
                     /locus_tag="VLB45_02445"
     tRNA            14608..14681
                     /locus_tag="VLB45_02445"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14642..14644,aa:Ile,seq:cat)
     gene            14716..14943
                     /locus_tag="VLB45_02450"
     CDS             14716..14943
                     /locus_tag="VLB45_02450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04594.1"
                     /translation="MQKPVIISIILGVGIVSAIVIFQASSICYPKRVEIDFDLKELES
                     GSMEYSSCLDLKHRIGIFNQKCDPDFNEIRC"
     gene            15012..15194
                     /locus_tag="VLB45_02455"
     CDS             15012..15194
                     /locus_tag="VLB45_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010191716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbtB-domain containing protein"
                     /protein_id="HSD04595.1"
                     /translation="MGVPMFAIAILLVIAGLGIFAVGFDQGHLFSIAFGQEAFDSLYL
                     HELTHDMRHAAGFPCH"
     gene            15394..15957
                     /locus_tag="VLB45_02460"
     CDS             15394..15957
                     /locus_tag="VLB45_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TATA-box-binding protein"
                     /protein_id="HSD04596.1"
                     /translation="MPQTKPIVSIENVVASASVDQKIDLNVITQTFPDVEYHPDQFPG
                     LVFRLKSPKTATLIFSSGKMVCTGAKSEDQAIKAVRSVVQKLRKGGIKIKKDAVIVIQ
                     NIVASASLGGKIHLEQAARTLPRSMYEPEQFPGLIHRMLDPKTVILLFASGKLVCTGA
                     KKESEVYRAVHNLHTLLEEKELMIYES"
     gene            complement(15980..16588)
                     /gene="pyrE"
                     /locus_tag="VLB45_02465"
     CDS             complement(15980..16588)
                     /gene="pyrE"
                     /locus_tag="VLB45_02465"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007549652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HSD04597.1"
                     /translation="MEFVKEFATFLYQNGAVKFGNFKLSSGKDSPYYIDLRVVPSYPH
                     QFRKMIKTFQNLISEKVGFDNFDCIASVPTSGLVIASALAIEIIKPLIYIRSKPKEYG
                     TENVIEGKIAKGTRVLLVDDVATTGLSLINAIKSLKNEGMVVSDAFTIINRFEGAREA
                     LAAENVRLYEILDIATLVTTLHQENFIDEQTLDRVKKQIGLI"
     gene            16639..17727
                     /locus_tag="VLB45_02470"
     CDS             16639..17727
                     /locus_tag="VLB45_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008300317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HSD04598.1"
                     /translation="MPEIWLSYGPTEVVLDIRAENLDKKIESKGTLLTDPEIGSRLDQ
                     LDLNKPTELVILDNSKAVQKVISVLFEKCNQNSLPKPKLLVDKPSMQQMKNIFSDPTL
                     TIAEFNDLELSNSNLVFVGEIEFDGLFGFNTISTKLVKKYGKEHMLAAYEKRKGNLPA
                     PGEELGNFPIAQKFTDSFEISAIEVVGNSVGVVDLAVGHPSSTSSISKSLLPIAINEV
                     GKHRTMIISPGKESGGETLGKALASLWNCAESIKDEGLAILLGECRRGVGSEAIQQYI
                     EGRMNLDRLKNPAKYVDGMEDLLFLTELEKKFQVGIVSILPEFYTKEKLGMLPFSGII
                     HAMDYILKTQGERQKVSVVSDGSHILLR"
     gene            complement(17719..18798)
                     /locus_tag="VLB45_02475"
     CDS             complement(17719..18798)
                     /locus_tag="VLB45_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010194342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HSD04599.1"
                     /translation="MSEPTQEEIVSMVSSIFVIRGISQNLDALQFEIEREGFKQKFVQ
                     FAQKIESKNLVARLESFDGRILLLVNRFKPPKSRQSWIPRLLFVATIIVVMIDGYYRT
                     SSANSIVWIGDPFEMAILYTGSLIGILGVHELGHIIAAKWHKIKTSWPYFIPGIPVIG
                     AVPTFGALIISRSFTINRDILFDIGISGPIAGLIVAIIVSTYGAYLSPTIPDSQASEL
                     FSQSELVEIYPSIIMSGTLLIAGKAIDGVEVIMSPILFAAWFGFLITFLNLLPAWQLD
                     GGHIARAMLGKKWHRIATYGSIGVLAALGYVIMALFVLMFSMRSQDPRPLDDISPLSR
                     NRKKIFIVAMCLAVLCAPLPFSILP"
     gene            18880..19380
                     /gene="rimI"
                     /locus_tag="VLB45_02480"
     CDS             18880..19380
                     /gene="rimI"
                     /locus_tag="VLB45_02480"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HSD04600.1"
                     /translation="MQVALREIGGCIIRRCDPSDLIPVMEINMKTLPEHYSDYFYESL
                     LAELPEAFLVAEIDRKLVGYIMCKIEYGFSNFKKLGFVKKGHVVSVAVLEENRKKGIG
                     RALVEEAIAGAKIKKADELYLEVRCSNNEAVRLYEKIGFIVKQRIKAYYRDGEDAYLM
                     AIEFSY"
     gene            19415..19639
                     /locus_tag="VLB45_02485"
     CDS             19415..19639
                     /locus_tag="VLB45_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016939814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HSD04601.1"
                     /translation="MTRQRSLGIFVFISCIVTFFIYAYLLMLSEWSPIVLQLSVLMAV
                     GGILGVVSWIGYNMATSKPSPSSLVSDDKK"
     gene            complement(19640..20467)
                     /locus_tag="VLB45_02490"
     CDS             complement(19640..20467)
                     /locus_tag="VLB45_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007549658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase family
                     protein"
                     /protein_id="HSD04602.1"
                     /translation="MLKEAVAGVLSEKEISELYGAFDQIGDIIIIRIPDSLVTKKKII
                     GEILLQQVKTAKSVFYQSSPVEGDFRTRNLELLAGEDRTETEYKEHGCRFKVDVQKAF
                     FSPRLSTERERIANLIEDGETVINMFGGVGMFSIIAAKKKKCTVYNIDINPHAAALCE
                     KNIKLNKLAGKVESIAGDATKIIEEKLKGKGDRVLMLLPERSDEFLDSALKACKKNGV
                     IHYYCHIHGERKNEVAKLAKEHFLEIIKIKSEVLGSKIVRPVGPRFYQAVVDAKISK"
     gene            complement(20473..22257)
                     /locus_tag="VLB45_02495"
     CDS             complement(20473..22257)
                     /locus_tag="VLB45_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis/translation initiation
                     ATPase RLI"
                     /protein_id="HSD04603.1"
                     /translation="MTHRVAVLDKELCQPKKCGLECIKYCPVNKSGADCIVLNEEIGK
                     AQIDENICNGCGICVKVCPFEAITIVNLAQELKTEKIHQFGINSFRLYRIPTPKKGRV
                     VGLLGRNGMGKSTIVSILSGNLKPNLGNYASPPEWNDILKNFQGTELKLHFEKIANEQ
                     IKASIKPQQVYNLTKVFDGTAKELLEKYDERNVMPELVRELGLQNSMDNKLNELSGGE
                     LQRLAIAVAASKDSDFYFFDEPSSYNDVFQRIGMARVIQSLAKLDKSVMVVEHDLTVL
                     DYLSDFIDVLYGEPAAYGIVADVLSTKVGINVFLDGYLPNENVRFRDIAFRFDASTSA
                     DEFVMTDNVVEYPALQRKYPNFSVSVDAGKVRKNEVLGVVGANALGKTTLMKMIAGVE
                     KPDFGKLESNVKIAYKPQYLTNDFDVEVISVLEKANGGGLEGTMEEEQVIDPLRLTKL
                     YNKSVKNLSGGELQKVAVVACLLQKVDLYALDEPSAFLDVEDRIAVAKLIQRFVRSYG
                     KSAIIIDHDIQLMDLISDSLVIFEGIPGKEGHGTSPMPKAQGMNRFLRSLDITYRRDE
                     TSLRPRVNKTESRLDREQKQSGHFYYRN"
     gene            22334..22453
                     /gene="rrf"
                     /locus_tag="VLB45_02500"
     rRNA            22334..22453
                     /gene="rrf"
                     /locus_tag="VLB45_02500"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(22456..22713)
                     /locus_tag="VLB45_02505"
     CDS             complement(22456..22713)
                     /locus_tag="VLB45_02505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04604.1"
                     /translation="MQKTTRLPKLSFSITPNPQGYMIACNEMPYLFTDAAKVEDIDEK
                     MKSLVEEYIGYFPDDAEKRGVNRSITVEAIWKATPNSVALD"
     gene            22767..22898
                     /locus_tag="VLB45_02510"
     CDS             22767..22898
                     /locus_tag="VLB45_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008300300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04605.1"
                     /translation="MASCAVCGEQFYDDIRFLNHMMEKHGFRNPNSNNAPDRNSRGY"
     gene            complement(22899..23285)
                     /locus_tag="VLB45_02515"
     CDS             complement(22899..23285)
                     /locus_tag="VLB45_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04606.1"
                     /translation="MDIQSLVPIMIYDDKCYLCGKFAKVVSLFARKKLLIVGHYTDLG
                     IEIKSEIFSDKYDPTKMFWFIDGKIAYGGRAALLPLITTILIGKIRSHTQNDIQASCS
                     AECKTPKAVFLRTKSLFSSSEKILIK"
     gene            23408..25075
                     /locus_tag="VLB45_02520"
     CDS             23408..25075
                     /locus_tag="VLB45_02520"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HSD04607.1"
                     /translation="MGLNYYKIIKSVREARKLVIKATATAGSGHPGGSFSMAEIMGCI
                     FHKFLRYDPKNPLWEDRDRLVLSKGHASPGLFSNLAVAGYFDKSEVETLRKFESRLQG
                     HPDFKCPGVEFCGGSLGIGLSFSIGMALAARIDQKQHRIYTIMGDGESDEGQVWEAAM
                     TASKYKLDNLTVFLDRNFIQQDDYTEKVMPLDEELIGDDISEMWKNAARWKTGDKWRA
                     FGWNVLEIDGHRVEQITKAVKSALQTKGTPSIIVSRTVKGKGVEHMEDNPKWHGVAPN
                     PKIAPIIDLELDCQFLISPSIIAGDMTNLENEVKRATHGRADFIHLDVMDGQFVQNTT
                     FDHNKIKELRSITSIPFDAHLMINEPVKHVRNYVEAGSDIITVHAEVCNESSFGEIHD
                     VLRANDVSVGLAINPDTELPSWAEKFIPDLDQLIVMSVVPGKSGQKYIELTHEKVQRL
                     SKVLAEKQFSGFIEADGGVDLSNVGQCFLDGARVFVGGSAIIGQKDVRATITEFRKKI
                     LYARRKLLIQKANELGGVDLVNKWIDLHEVGKKKTELLQIAKEAGFT"
     gene            25072..>25872
                     /locus_tag="VLB45_02525"
     CDS             25072..>25872
                     /locus_tag="VLB45_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HSD04608.1"
                     /translation="MTEMTDMRTVYGETLIKVGEENPNVVVVGADTTDSLKTKPFGKK
                     FPNRFFNVGIAEANLVSVAAGLAIEGKVAFASTYAIFLPGRCVDQIRNAIAYPSRGDK
                     KGLNVKMVVSHGGLSVGADGGSHQQIEDIAIMRTIPNMKVIIPADTVTVSKLTWIMSQ
                     TYGPFYMRMARSKTPVVHSEAQEFEIGKGIVLRDGSDCTIAACGITVPMALEAAETLK
                     QEGISCRVLDLFSIKPIDSILIEKAARETGGIVTCEEHNILAGFGSAVS"
BASE COUNT         8035 a         4669 c         5101 g         8067 t
ORIGIN      
        1 gaatagtaga agaggtagag aaagcacttg acattgctca tcagataggt taccctgttt
       61 tgctcaagtc tgtatttgga ggtggaggaa gaggaataag attagtccat aatgagaaag
      121 aacttaggga agcctttgaa attgcatcag gagaatcaaa agcagcattt ggaaaatccg
      181 cactttttgt ggagaaattt cttccaaaaa tcagacacat agaatatcaa ttattcagag
      241 ataagcatgg aaacgctgta catatttttg aacgagagtg ttctattcaa agaagacacc
      301 aaaaactcat tgaaatgtct ccatcacctg tggttgatca aaagacacgg gatgaaattg
      361 gaaaacttgc catcaacgca gcaactgcag ttgattatac aaatgctggt actgcagaat
      421 ttctaaggtt agatgatgga acattttatt tcatagaaat caacgcaaga cttcaggtag
      481 aacatcctgt aacagaactt gtttcaggct tagacttggt aaaactgcag attgatattg
      541 caaatggaaa agaaattcca ttcaaacaaa aagatttgaa gctaaaaggt tgtgcaattg
      601 aatgcagaat aaatgcagaa gatacatttc ttgattttgc gccatctaca ggtccaattc
      661 ctgacgtgaa cattccgtct ggtccaggag ttcgcgttga cacttacctt tatccaggtt
      721 gcactgtttc tccatactac gactccttaa tggcaaagct tgtcacatgg ggaggaacct
      781 ttgaagaatc acgacaaaga atgcagcttg cattatcaga tttttatatt gagggcgtag
      841 agacatcgat accactttac aaaacaatac ttgatagtga agaattcatc aacggtgatt
      901 tgtccaccga ttttcttgac agattcaaaa tcttggacag actacgggat gatatcaaaa
      961 aaaatatctc ctacaatact gatgcagctc tggcggcagc agtaattcat tctgaatttc
     1021 ttaaaaatag aataaaatca cgtgtagaaa ataacatccg ctggaagagt caactggata
     1081 gtgcataatc gcatggattt tagaattgaa aatcttggaa gcaaatttga tgctgaaatt
     1141 attcgaagtc ttggtaactc agaatatgtt gtaaaaatac aaggaaagga aagaaatctc
     1201 aagattctca acatgacttc tgataaaata gaattcatgc ttgataatac tcatcacact
     1261 gtcaaatatc ttgataataa tactgctaaa atgacactgg ttgtaaatgg cgtacagata
     1321 acatttgata aacatacatc tcttgatgaa attgtttaca aaaattctgg ctctgaatct
     1381 ggtggtgatt cacaattaag tctgcgaagt caaatccctg gtcgagttgt atcaattaac
     1441 acaaccgttg gaaaccaagt caaaaaagga gatgtgttgt gtgtattaga atccatgaaa
     1501 atgcaagttt caataaaatc tcataaagat ggcgtaatca agtctatgaa gatcaaacaa
     1561 ggcgcatcag tcgcaaaaaa tgatgtatta atggaaatag aataaataat tctaaaataa
     1621 aaacaaagga actaattccc tttgtttaga aagttcatta tctcttggta gtttggttct
     1681 attagcggat tgtcagaaac ccatttgtat actattttgc cgccctcatc aacaatgaaa
     1741 acagaacgct ttgctgcgtc atatcctttc acatgcaaga gatctggcat tagaacatca
     1801 taatctcgta ttgtttgact cttgtagtct ccaagtactg gaaaattcag atgatgtgtt
     1861 tctgcaaaag ccttgtttgc aaatggacca tcattactaa ttccaataac ttgagcgcca
     1921 agttttgata tctgttccca tgaatcccgg aaagtacaca tctcttttgt acagacaggt
     1981 gagcttgctg ccacaaaaaa tgatagtaca acttttttac ctttgaactc atcaagactt
     2041 cgcatcttca agtcagtatc taccaaggta aaacttgggg ctttatctcc tacgtttacg
     2101 gtcatacata agaggttcaa actgtcatat atcaatcatt aagatttgtt tggtgtatca
     2161 ataaatacaa gagatataca gtaactttat gccaagtatg aaatgtgttt catgtggaat
     2221 tggatttttc tctccaacag gctctgacaa atgttcaagg tgtgtggaaa aaggttcaca
     2281 tggaacagaa gaaagtcacg actcttgtgg ttgtggtcac tcacatcact aatttccatt
     2341 ttttcttatc atactctaaa tctaggaatg tgcaaaatta agatagttgt gaaacttagt
     2401 tcaatgaaaa aatgatcgtt aaatgtgcat agatttttat actctccgac cggcttttta
     2461 gctactttgg taggagaggt atctggcagc tttagccctg tattgttgat gtttggattt
     2521 gcaattgtag caactgcccc cgcacttatt ctttctagaa tgatatctcc aagaaaaaga
     2581 agtaatcctg ttaaatttct tccaatggaa tgtggtcaag taccaagtgg ggaaggtaga
     2641 actcatttca tgatgcaata ttatgcatac gttttgatgt ttgtagtttt tgatgtcatg
     2701 tctattttct tatatgcatg gggaacaacg ttgttaaatc tcccaaaatc tgcaactctt
     2761 ccaatcatag catttttagg aataatgttt gcctcatttg catttgcact ataccaatca
     2821 gggaggagaa atatttggta attcttaaat caaatatgac atataatcaa gtcaagaggg
     2881 acaaaaattg caaaaagaac taatctctca gaatgcagca aatgtatttg ttggaaaact
     2941 aggagatatt cttgttaaag caattgacaa gccacttggt tatgcaatta actggggtag
     3001 gctgtggtca ctttggcccg tacacatcga gactgcatgt tgtagcgtag aatttggagc
     3061 tgcatcaggt ccaaggtatg atgtagaaag atttggaatc attgaagcct ttggttctct
     3121 aaggcaatgc gatctagttg tagttcaggg aactattacc agaaaaatgg ctccaagact
     3181 cagaatggtt tatgatcaga tgccagaacc aaaatatgtt atcgctatgg gtgcatgtgc
     3241 aatcacgggt ggattgtact ttgattcgta caatgttctg cctggaattg atggaatact
     3301 tcctgtcgat gtttacgtac cgggttgtcc tcctagacct gaaactctta tacaaggatg
     3361 catgctgctt caagaaaaaa ttaagagaat gaaggctagg taataatgag ttctgaaaca
     3421 actgatgtaa aacccagtga aactgtagga acaaagccgg atgttgccaa aaaagaaact
     3481 gtatccatag cagaaccaac atttgaaaaa tctcttgccg aaaaaattac ttccaagttt
     3541 ggcacaaaat ctgttatcgg ttttatcaaa ccaaatagaa ttagaataaa cacaaagaaa
     3601 gaagatattc taaatatcgc agcatacatt cgtgatgaac tgaactttga tcatgcagaa
     3661 tctgtaacag gtgtagatta tcctaattca aaagaaattg aggttgttta tcacttaggt
     3721 tcatattctg atccacaact tgcaaagcaa atacttactt tagcaactag actgccaaga
     3781 gaagaaattc caaatcctgg aaatgatagc acaagaatgc cgtcccttag agatattttc
     3841 tatagcgtag agtttcatga gagagagtgt tttgaaatgc ttggcgtata ctttgaaaat
     3901 catcctgata atagaagatt acttttgcca gaagactggg cagatatacc accaatgaga
     3961 aaggacttca aaataaaggg aagataaaat gacagtacga ctacctccag gcattcaact
     4021 tgaaaaagca gatgaaagga taatgacgtt aaacgttggt ccacaacatc ctggttctgg
     4081 ccacatgcga cttatcatca aagttgatgg agattatatt gtatcttgtg atcctgatcc
     4141 tggatatgta catcgtggag aagaaaaaat ggcggaatat agaaactaca tacaaaacat
     4201 tccacatctt gaaagacctg taattcatga ttcttgcaat atactgtatc cgtattgtct
     4261 tgcagtagag gatcttttgg gaattgaagt accagagaga gcaaaataca tccgtgtaat
     4321 tgcatccgaa ctaaatcgtt gtgtgtatac tctttattgg cttgcaattt acggaatctt
     4381 tctgggtcat tctacaatgt tcatgtggcc agctggtgat agagaattgt ttattgatct
     4441 tttggaagca atgtctggag ctagagttac acatgcatac tttgtaccag gtggtgtacg
     4501 aaatgatcta cctgcaaact ttgaagaacg atgcttaaaa caagtggcct actttgagca
     4561 tagattaaaa caatatgaag atattttcta ccaaaatcct gttctaaaac agagaacaga
     4621 gggaactgga atcttatcaa gaatggatgc aattagactt ggaactactg gctctgttct
     4681 tcgttctgcc ggggtagact ttgacatcag aaagaaagag ccttacgatg tttatgagaa
     4741 cttggatttt caatcacaag tactcaaaga aggcgattct tacgcaagat cacgagttcc
     4801 ctatcttgac atgcttgaaa gctgtagaat aataaaagat gcattgaaaa aaatgccaaa
     4861 gtcaggttct gtaagagtaa aattaaaacc taatccaaaa ggtggaaatg aagaagtgta
     4921 tcgtagagta gagtcaggaa ggggttcact tggaatgtat gttgtgtcag acggcaaacc
     4981 tgaaccatat agagtgaaaa tcagcgtagg ctcatttaga aatctcatat gcatgccata
     5041 tttgcttaaa ggagaaaagc ttggaaacat gccagctgtt tattggagcc ttaattattg
     5101 gccagtggag gctgatagat aaatgtctac aattgcacca gagtttagat taagcagatt
     5161 tatcgcatca ctaacagatc tgatcttttg gattgtggtg ttgtttacac tcattggtct
     5221 accagctgca atgatcgcac tcttttacat tgaattgcca gaaattaatg gtactctctt
     5281 aacaccatat cttgcattga cgtggatagc tgatccatct agaactttac caattataaa
     5341 atcgctaatg caaactgatt tttttagaat taccacattc cctggatttg gctttgctgc
     5401 gcttcttgca gcggcaacaa tattcataga aagaaagttt cttgcgaaaa tgcaacttcg
     5461 tgtaggaccg ctttattgtg gaaagataga aggaatttta caactgatgg gtgatggact
     5521 aaaattgata tcaaaagaaa taatcattcc agccaaagct gataagccaa ttttctgggc
     5581 tgcaccgttg ctatttgtag gaactgctgc ggcattcatc tcattgatcc cagttgcacc
     5641 aggctgggtt gttgcagaca ttgatgtagg attattggct gtatttgcag tcatcggctt
     5701 ctttccgata attaccgttc tctcatcatg ggcttcaaac agtaaattcc cattcattgg
     5761 aggaattaga gctcttcacc aaatgatctc atttgaaatt ccactaatac tgtcgttgct
     5821 tggaattgta attttaacag gaacactaaa tctcactgaa atcgttgaat cacaacaaca
     5881 ctattggtgg attatattca tgccaatagg cgcaatcgtg tttttcacca caatacttgc
     5941 agagctagaa agaattccat tcgatcttcc agaagcagaa agtgaaattg ttgctggctg
     6001 gttgactgaa ttttctggta tgatgtacgg gttagttcaa cttggctctt atttgaaatt
     6061 gtatgcattt gctgcactgt ttacgatttt gtttttgggt ggatggaatg gtccaatggt
     6121 atggccgcca ttcccagagg aaataataac acatggaatc acaatgggtc caataacagc
     6181 tcagattcca ggtcttccat tactagatca agaaatgcta aatgcaactt tatggttcac
     6241 aataaaaaca gttggagtca tacttgtaat cttacttcca agaggtgtaa tgccaagaat
     6301 tagaatagat cttcttttgc acaccggttg gtacaaacta attggattag cattcgttaa
     6361 catctttata gctctagctt tgtactatgc aggtgtcttg ggtccgggag gattgataca
     6421 atgactagtg caggtggaat catacgcgct cttaattctg gtgtaaaaca ccttgcagtt
     6481 aaacgattta cattacgtta tccagaacaa aaactaaagt ttgtaggcga tggctttcag
     6541 ttcgatcctc agactggagt aggaattgct ggtctgaagg gacgacacat actattccat
     6601 gataagtgca ctggttgcca gctctgcgct atcgcatgtg aggggattgc cgaagccatt
     6661 gcaatggtaa aagtggatga aacttggaaa cacaacaaga aagcaataat gccgcagatt
     6721 gattttggaa aatgtgtatt ctgtggatta tgtgtagacg catgcccatt ttatgcatta
     6781 tacatgacaa atgactatga gctttcttca tttacaaaag aagggttaat ctatacaccc
     6841 ggtcagctcg ctgtaatccc aaaggtagac caagatgtag aaataaaaat agatgaccgg
     6901 ggtgctacac atggctgatg cagtatttct tgcgatttct gttttaacaa ttggttctgc
     6961 aatagcagca cttgaaatcc gttcgatgat ttacggttca atagctttga tggttacact
     7021 tgcaggtgtt gcaggatttt tcttattgct tgatgcccca tttgtggcaa tgtttcaaat
     7081 ctcagtatat gttggttcaa tcgctgtact catactattt actgtaatgc ttgtaagacg
     7141 tgaactgatc tttaacaaag ttgaagatcc tagaagaaga tacgccggaa ttggtttgat
     7201 gattgtaatt atgcttggaa ttggtactat aataattggc tctggaataa aatcaatgga
     7261 agtagacgca cctgcagtcg actttagaga aatcggtgaa gactttctta catattattc
     7321 gccagcacta gttgtaatgg cgcttatcct ggcatctgca gttatcggag ctttagcctt
     7381 ggcaagaagg gaggatgtag caaatgagca acgaactgat tgaatttgtt ctagtatcgg
     7441 tagctctctt agctatcgga gtatacggac tagcagtaaa aagaaatgct atcagaatgc
     7501 tttttgccat agaaatgata gtgaatgcgg caaatctcaa cttagttgca ttctcaagat
     7561 ttatcccaaa cagccaagga cagacattag cgctattttc tatagctatt gcagctgctg
     7621 aagttgcagt agggttggca ctagtaattg ttgcatatag aatgtacaaa aacatcgata
     7681 ttgcagactt taggagcttg aaaggataat gcaatatctc ttgttgcaag cggtcttttt
     7741 accgctacta ctgtcacctg tagcatatta tcttggaaga aaatctggac ctaatgcagc
     7801 aacttggttt acttttggtg tcttacttta ttgtacaacc atactgattg ctccagtgtt
     7861 ttctggaaca tatgaagaac actatccatg gactcaaatg tttggagaat ttggattctt
     7921 acttgatgga ctagcatccc catttgcaat cataatctat gttctatcca cagtacttgc
     7981 attgtactct aaaccataca tggttgcaaa aataaaagaa cagtttgaag aaaaaatgca
     8041 tcaccaaaaa cagatgattg gtgatggcca atcaactgcg atgatcgaat catcggcttt
     8101 gaaaggatat ctgaataatc aaatcggaat ttactttgcg ttataccttg tttttgcaat
     8161 gggaatgctt ggaactgttc ttgctacgaa tctgatagaa ttttatgtct tctttgaagt
     8221 gatgctaatt ccaggtttct ttttaattgc attttatgga tatggtgcaa gaagaagaat
     8281 tgcacttttg ttctttttct ggactcatgt aggtgcagta gttttacttc ttggcttttt
     8341 agcaatagga cttagtattg gaagctttga ttttgttgac atacgagaag aaggtgtacc
     8401 acctgacgtt cttgcgtttg ccgccgttgc tatagtaatt ggactagcag taaaacttgc
     8461 cgcatttatc ttccacatat ggctaccata tgcacacgcc gaagctccta ctcctattag
     8521 cgcactcttg tcacccgcaa tgatcggaat tggagcatat ggtcttttca gattattagt
     8581 tatgcttttg cctgggcaat acgaaaactt ggcactttat ctgaacttgt ggggattagc
     8641 tacgatgctt tatggtggtg caatggcact aatgcaagac gacatcaaaa gattatttgc
     8701 atattctagt atcagccaga tgggatatct gttatttgga attggttctg cttctgcact
     8761 tggacttgct ggttctgaga tgatgtatgt atcacacgga ctaggaaagg gactgctttt
     8821 catgatggca ggtgccatca tactacaagt tggcacacga agtatgacaa aacttggtgg
     8881 gcttgcagga aagatgccaa ttactgcagt ttgtgcgatg attggtgctc ttacaatcat
     8941 gggcgttcca ccaacaagtg gattcatggg agaatggact ctgttctatg gtgcactaca
     9001 aactgcaatt caagaaggtt caacaatccg aatgattgca tttggtctag gtcttgtatc
     9061 tactgtcatc acgatgtcgt acatcttatg gatgatgaaa agaatatttt ttggaaaact
     9121 tccagaaaac ctttcaaaca caaaagaagc aaattggtat atcaccgcac cgatgatggt
     9181 actagcagga ttttcaatag tcgtaggaat atatcctgac atattcttca accaaataat
     9241 tcctttcatg aatggagtaa tggggatcta gtttatggca tctgaaattc ttggatttgg
     9301 tttagaagca gggggcatga gtgcttgggc aatttggatc ctacctttca ttgcagcttt
     9361 gataattcct ggggttgcca aattctcaaa acgtgctact agtggaattg ctgtaggctt
     9421 tgcactcatg agcgcacttt ctgcaacaac acttttgcca gcagcacttg aaggatatga
     9481 aacacatgac caaatctcat ggatatcatc aatcggaata aaggcaggtg tcttggctga
     9541 tccgcttgca gttttaatgg caaatgtagt agcatggatt tcatttttga tcatggtgta
     9601 cagtactggt tacatgaaag gtgacaggaa cattactaga ttctggttct ggatgatgtt
     9661 tttcattggt tccatgcagc taattgtttt gtctgataat ttcttgcagc tcttctttgg
     9721 gtgggaagga gtaggacttg catcatatgc tttgattagt ttttggtatc aagacaaaaa
     9781 gaaagatcat gttggaacag aaggatacac cgcacttggc gtattacaat attattctcc
     9841 aacacatgct ggaatgaagg cattcatcat gaccaaggtg ggagacatca tgatgcttgc
     9901 tggaatgttt ttgatatttg catttgctgg aacgtttggg tttagagaat taacaaatga
     9961 cactgcatgg gcaactacca tgtctgctca aggcctccta ataccagcag cagtattgct
    10021 ctttggtgga gcagttggca aatcagcaca gtttccatta aatgaatggt tacttgaggc
    10081 aatgactggt ccaactgcag tttctgcttt gatacatgca gctacaatgg taaaagctgg
    10141 agtctttttg gtagcacgat tgggtccgtt gttttttgcc cttgcagcag taggagttgg
    10201 tatggatcaa ttctttgaaa ttgttacatg ggtaggtgca atcactgcac tattgcttgc
    10261 tacacaagga atggtttcac ctgaaatcaa aaaagtactc gcatactcaa caggttcaca
    10321 aattggatac atgatgatgg cacttggaat tgctggactc tcacaacagt ttgttgatgg
    10381 ttacactgca ggattcttcc atctgatttc acatgcaatg ttcaaggctt cattgtttat
    10441 ggctgcaggg tcattgttac atgttgttgg ttctagattc atgactgaca tgggtggatt
    10501 acgaaaacat atgcgaaaga catatgcctt catgtgggca gccggtcttg cattaatggg
    10561 tgcaccattt atcacaactg gattctggag caaagatgca atctttgcag cagtatatga
    10621 gtctggaaac acttgggcaa ttccaatatt tgcaattgcc cttgttactg cgataattac
    10681 tgcattctat acgacaagaa tgattggtat ggtattcttt ggaaacaaga gcaagcacat
    10741 tgaaaaaatg gaaaaggaag gacatcacat acatgaagca ccaaaatcaa tgtggattcc
    10801 atatggaatc cttgccgtac taactatagg cattggtata attggattgt ctgctgaaca
    10861 aggaattcat cacctgttta cagaatatct gtctcacacc tttggaatag aatctgctca
    10921 tgaagaagtt gcactggaat cagaggcaat gcttggagga tttctaagtg gcattaatcc
    10981 aatagcactt gtggcttcat tacttgcatt tgcagttgga tttggtcttg gatatatctt
    11041 ctatattgga agatttgtag atccggtgaa atttgttaac tctaatctat tcttctatgg
    11101 aatacacaaa tttttcttaa acagatggta cctgaattct atcatttact ggtgctttgt
    11161 aattgcacca ttgtactttg caagaggaat ttggagatac atggaaagca ctgtcattga
    11221 tggcatgaac aaaggttcag aaaaagcagt tggttggagt gccaaagtaa ttcaaggcac
    11281 tcaaaccgga atttcacaat cttatttgta tgtatttgga gcaggaatac tctttgtagt
    11341 attgcttttg ctaatttagg agaaaagaaa tgatagactt tacttcaaca ccaatcattc
    11401 taactgtaat acttggcgca attggcgtaa ttttaccagt aattaatgta ataaggaaag
    11461 acaaaggctc gtcttcattt tatggtgcaa ttacttttgg agctttactt gcagcaattg
    11521 gatttgtcat atatcaaata atatccaatc atgttatacc tgctgcaata ttctcagaaa
    11581 atgttctagt cgatgatatg tttggtgcat tctttgcaat agccatgctc attgtagcaa
    11641 taatgacaac tgtaggctct tttaattaca tgcgaggtaa ggctcatccc gcagtatact
    11701 attctctgat tctcctctca tcaatcggaa tggtactcat agcatattct acagacttgg
    11761 taatgctatt ggttgcttgg gagctcatga gtattccaac ttacgttctt gcgggattta
    11821 tgaaaaagga tccatcgtca aatgaagccg cactcaagta ctttttgttt ggagccttgt
    11881 catctgcaat tataatttat ggaatttcaa ttgcatatgg attaacaggt tcaacaaata
    11941 tttcagaagt gatatctgga ttttctaatc tgcaatccga catgatgccg cttgctcttc
    12001 ttgccgtagc catgtttatt gcaggatttg gattcaaaat gggattggtg ccatttcaca
    12061 tgtggttgcc tgatacatac gaaggttctc ctactacaat ttctgcactt ttggctgctg
    12121 gtacaaagaa agcaggattt gctgcagctt tgcgtgtagt cattatggga acagttgcac
    12181 taaacatgga atggactttt gcacttggag ttgttgctat tatgacaatg actgttggaa
    12241 accttgctgc aattatgcag aaaaacatca caagaatgtt agcatattct agcattggac
    12301 aagcaggata catcttgatt ggtatgagta ttgcaccatt ttcacaaatc ggattacaag
    12361 catcacttta ccacattcta aaccatgctg tgatgaaagg tgctgcattt attgcagttg
    12421 caggaataat tgcaactctg gcagtttccc accttgataa aatcaaaggt cttggaaaga
    12481 gaatgccgat aacatcgatt ggactagtca tatcactact tgcattagct ggtgttcctc
    12541 cattgaatgg attttggagt aaattgatgt tatttggctc ggctatagat gcaggttctg
    12601 ttgtctcatg ggctccgtat cttgccatag caggagttct aaacagtgcg ctatcgcttg
    12661 cttactatgg ttggattatc agaaaaatgt actttgaaga aggcgagtct gagaagagag
    12721 taaaagaacc aaaatcaatt atcgcagtaa tgatattctc gataatcttt atggtggcaa
    12781 caggagtata tccagatcct atgattcagt ttgcacaatc tgcagttcca aacttgacaa
    12841 tgcctactgt accttaaaca ttgtaaggtc tacaagattt tccaaatgag atttggcagt
    12901 acactcttct atctttcgta gaccagattt tgattttaga gaataatctt tcaacaaata
    12961 atccatgtgg ttaaaaacat cacgaggatc tgagctcttt tttattagga cattaaacaa
    13021 cttgtcttca ttattccaat cttgcaagtc atcatgaatc tgatatgcaa ttcccacatt
    13081 ctttccatat tcggcaagtg caagtatttg ttcttctgta ccaccaccaa gtatggcacc
    13141 aatttttgat gcagcttcaa aagcagttgc ggttttgtat tctatgactt taagataatc
    13201 atcaaaagta acatcttcac ttgattctaa tctagcttcg atcatttcgc catcactcat
    13261 catcatggcc gtagtagcaa gctcacttgc aattctcgga ttatcgattc ttgatgtaat
    13321 gtttaggatt aatccaagca caaaatcgcc agtaagaata cttgtattat atccgtattt
    13381 tatgtggaag ggatcttttc ttcttcgtaa tgtctcatta tctattatgt catcatgtat
    13441 cactgattct gtatgtagta actccacggc acaagctgca gtataggcgt tttcatcaca
    13501 aattccaaca ctttctgcag atagcatcaa aataagaggt ctaatgcgtt ttcctccatc
    13561 caatgcatat tgtaatggtt caacaaattc tgatgcagag taaagttcaa gctcatgttt
    13621 taatgctaaa ttgattttgt cgagatattg tttgtagtct tttatgagag gattaatttc
    13681 aaaatttttt ctatccacac acttgcactg ttaagcttaa cctgatgtta gaatattaat
    13741 gctttgtagg tgctccagcc gggattttca tcataaagaa tttgaacccg ggatcaccgc
    13801 cttgaaaggg cggtatgctt gaccggtcta caccactgga gcccaactgc atcctgtttt
    13861 ttgtccataa aatccttttg aagtgttcaa aagatttttt tattggctgg atttcattaa
    13921 tcgcatgaat tcactagtca aaagaataga tgagttggta gaaaaacaaa gtttgctaaa
    13981 acaccaattc tacgtaatgt ggtctgaagg aaaattaacc cttaattctc ttaatggtta
    14041 ttcaaaagaa tattttcaac tagtcaaggc agtaccatca tttgttggtg cagtaatgca
    14101 acaaagtcca aactcgatta aaaatgagct tgcagctaat caaaaagaag aaacagaaca
    14161 cataacacct tggatgaaat ttgcaagtgc actgggagta tcacaaaccg agttggaaaa
    14221 atattctggt cttgtaaaga caagaaaagc tgtagcagaa cttgcgtccc ttatgaattc
    14281 ttacgaggat ggtgcagctg caatgtatgc attagaacaa gagattccaa aaattagcct
    14341 ctcaaaaatt gatggtctga gaaagtttta tggtttgaca agtgacgaag caattgagta
    14401 ttttagattg cacgcagaag ctgacatcag acatgcagca acttggagga gagttctaga
    14461 aaatgtttca gatgaaaaag aagaggaatt attccaaact gcaagcagat ctattgccgc
    14521 tcaaaacatg ttgcttgaca gttgttatga agcatactgt tgatctctaa taccattttt
    14581 atactccatt caaacctgtt ttttcgtggg cccataactc agcttggtag agtaaccggc
    14641 tcataaccgg tgagccaagg gatcgaagcc ctttgggccc acttcttttt aatttagtaa
    14701 atcacatcaa ctttgatgca aaaaccagtc atcatttcaa ttattctagg tgtaggaata
    14761 gtgtctgcga tagtaatttt tcaagcatct agtatttgtt atccgaaaag agttgaaatt
    14821 gattttgatc taaaagaact tgaatccggt tcgatggaat attcgagctg tcttgatctt
    14881 aaacacagaa ttgggatctt taatcaaaaa tgtgatcctg attttaatga gatccgatgt
    14941 tgattaaacc ttatataacg agttttctca gaaacttcat ggtcaataca agcctactca
    15001 caacaaacaa gatgggagtc ccgatgtttg caattgcaat cctcttggta atcgcaggtc
    15061 ttggaatttt tgcagtagga tttgatcaag gtcatctctt tagcatagca tttggtcaag
    15121 aagcttttga ttctctttat cttcatgagc taactcatga tatgagacat gctgccggct
    15181 ttccatgcca ctagttttaa aataagcagt agctgatttt cattgggctg caatgaagaa
    15241 tcagagttat atctgaacta atgatgtgaa ggcatttgtc tgagctgcct atgcagtttt
    15301 ttcatggaaa tgatgatttg ataaatttga cacaaacagt actgggcaaa aacattatag
    15361 agtatatata aagcccttta taaggaagca aacatgcctc aaacaaaacc aatagtcagt
    15421 atagaaaatg ttgtggcttc tgcgtctgta gatcaaaaaa ttgatctcaa tgtcatcacg
    15481 caaacttttc cagatgttga gtatcatcct gatcaatttc caggactggt cttcagatta
    15541 aagtcaccaa aaactgcaac actgattttt agttctggca agatggtttg taccggagca
    15601 aaatccgaag atcaggctat caaagcagta agaagcgtag tacaaaagct tcgaaaaggc
    15661 ggtatcaaaa tcaaaaagga tgctgtcatt gtaatacaaa atattgtggc atctgcaagc
    15721 cttggtggta aaatccactt ggagcaagca gctagaacct tgccaagaag tatgtatgaa
    15781 cctgagcaat ttcctggttt gatacacaga atgcttgatc caaaaactgt gatcttgctt
    15841 tttgcatcag gaaaacttgt atgtacagga gcaaagaaag aatctgaagt atacagggca
    15901 gtacacaact tgcatactct gcttgaagaa aaagaactaa tgatctacga aagctaaatc
    15961 tgtaagcatt ttttgcgctt cagattaaac ctatttgttt tttgacacga tccaacgtct
    16021 gttcatcaat aaaattttcc tggtgtaaag tggttactaa tgttgcaatg tctaatattt
    16081 catataatct gacattttct gctgctaaag cttcacgcgc tccttcaaat ctgtttatta
    16141 ttgtaaatgc atccgagaca accatgcctt catttttgag tgactttatg gcattaatca
    16201 aggaaagacc agtcgtagca acatcatcta caagtaaaac tctggttcct tttgctatct
    16261 ttccttctat gacgttctca gtaccatact cttttggctt tgatcttatg tagatgagag
    16321 gtttgataat ctcaatagca agtgcagaag caatcacaag acctgatgta ggaacagaag
    16381 caatacaatc aaagttatca aatccaacct tttctgatat taaattttga aatgtcttta
    16441 tcatctttct aaactgatga ggataacttg gcaccaccct tagatctatg taataagggc
    16501 tatctttgcc gcttgaaagc ttgaaatttc caaattttac cgcaccattt tggtacaaaa
    16561 acgttgcaaa ttctttgaca aactccacag caaaattaac tatatcagat ttattttaat
    16621 tgtcctattc gtagtcttat gccggaaatt tggcttagtt atggcccaac agaggttgta
    16681 ttggacatac gggcagaaaa tcttgataaa aaaatagagt caaaaggcac acttttaaca
    16741 gatccagaaa tcggctcaag acttgatcag cttgatctta acaaaccaac agagctcgta
    16801 atactggata attcaaaagc agtacaaaaa gtaatctcag tattatttga aaaatgtaat
    16861 caaaactctc ttccgaaacc aaaacttctt gtagataaac caagtatgca acaaatgaaa
    16921 aatattttct cagatcctac tttgacaatt gcagaattta atgatcttga actttcaaac
    16981 tctaatctgg tatttgttgg agaaatagag tttgatggtc tgtttggatt taataccata
    17041 tctacaaagc ttgtaaaaaa atatggaaaa gaacacatgc ttgctgcata cgaaaaaaga
    17101 aaaggaaatc ttccggcacc tggggaagaa cttggaaatt ttccaatagc acagaaattt
    17161 actgattcct ttgagatatc tgcaattgaa gtggtaggaa attctgtggg tgtagttgat
    17221 ctggctgtag gacatccatc ttctacctca tcaatatcga aatcgttact tccaattgct
    17281 ataaatgaag ttggtaaaca tcgtactatg ataattagtc ctggaaaaga aagcggaggt
    17341 gagacacttg gtaaggctct cgcttcacta tggaactgtg ctgaatcaat caaagatgag
    17401 ggccttgcaa ttcttctagg cgaatgtaga agaggggtgg gctctgaagc aatccagcaa
    17461 tacattgaag gaagaatgaa tctagataga ctaaaaaatc ctgcaaaata tgtagacggg
    17521 atggaagatc ttttgttttt gacggagctt gaaaaaaaat tccaagtagg aattgtttct
    17581 atattgccag agttttatac taaagaaaag cttggcatgt taccatttag tggtataata
    17641 catgcaatgg attatatttt aaaaacacaa ggggaacgac aaaaggtttc tgttgtttct
    17701 gatggttcgc atatcctgtt aaggtagtat tgaaaacgga agcggcgcac ataataccgc
    17761 aagacacata gcaactatga aaattttttt tcgattcctt gaaagtggag aaatatcatc
    17821 aagcggtcta ggatcttgac ttcgcatact gaacattaaa acaaaaagtg ccattataac
    17881 atagcctaga gctgcaagta ctccaatact gccatatgtt gcaattctgt gccacttttt
    17941 tccaagcatc gcacgagcaa tatgaccacc atcaagctgc catgctggca atagattaag
    18001 aaatgttata agaaatccaa accatgccgc aaaaagaatt ggtgacatta ttacttcaac
    18061 tccatcaatt gccttgcctg ctatgagaag tgtaccactc atgattatgc ttggataaat
    18121 ctcaactagt tctgactgtg aaaaaagctc actagcctgt gaatcaggta tggttggaga
    18181 aagatatgcc ccataagttg aaacaattat tgcaacaatc aagcctgcaa ttggtcctga
    18241 aattccaatg tcaaacagta tgtccctgtt tatagtgaaa ctgcgtgata tgatgagtgc
    18301 accaaatgta ggtactgctc caatgactgg tatgccggga ataaagtaag gccaacttgt
    18361 cttgatcttg tgccattttg ctgctatgat gtgacctagc tcatgtactc caagtattcc
    18421 aattaaagat ccagtataaa gaattgccat ctcaaatggg tcacctatcc aaactatgga
    18481 atttgcacta gacgttctgt aatatccatc gatcattaca acaattatag tggctacaaa
    18541 aagtaatctt ggtatccaag attgtcttga tttgggcggt ttgaatctat tgactaaaag
    18601 taaaattctg ccatcgaaac tttctaatct tgccactaga tttttagatt ctattttttg
    18661 cgcaaactga acaaacttct gtttaaatcc ctcccgttct atttcaaact gtaaagcatc
    18721 aagattttgg ctaattcctc ttataacaaa tatggaagaa accatcgata ctatttcctc
    18781 ttgtgtaggt tcgctcaata gatagattgc aatttagtgt aaattaattc ttttactaac
    18841 ttaaggttaa ataattgtca tgtccttgat cattttgcat tgcaggttgc actaagagag
    18901 attggaggtt gtattatacg aagatgtgat cctagtgatc ttattcccgt aatggaaata
    18961 aacatgaaaa ccctgcctga gcattattct gattatttct atgaaagtct gcttgcagag
    19021 cttccagaag catttcttgt tgcagaaatt gacagaaaac tagtaggtta tatcatgtgt
    19081 aagatagaat atggattttc aaattttaaa aaactgggtt ttgtcaaaaa aggacatgta
    19141 gtatctgtag ccgtgttaga agaaaaccgc aaaaaaggca ttggacgagc tcttgttgaa
    19201 gaagcaattg caggtgccaa gatcaaaaaa gcagatgaat tgtaccttga ggttcgttgt
    19261 agtaacaacg aggcagtacg actatacgaa aaaattggat ttattgtaaa acaaagaata
    19321 aaggcgtact atcgtgacgg agaagatgcc tatcttatgg caatcgaatt ctcctactag
    19381 attaaaataa ataactcaga aaaagagtag cgctatgact agacaacgca gcttgggaat
    19441 attcgtcttt atttcttgta ttgttacgtt ttttatctat gcgtatcttt taatgctctc
    19501 tgaatggagc ccaattgttt tacaactctc agtgttgatg gcagttggag gaattctcgg
    19561 agtagtgtct tggattggat ataacatggc tacatcaaaa ccgtctccat catcgttagt
    19621 atcagacgat aaaaagtagt tactttgaaa tttttgcatc taccactgcc tgatagaatc
    19681 taggtcctac tggtctgact attttagatc ctagaacttc tgattttatc tttattatct
    19741 caagaaaatg ttcttttgca agtttggcaa cttcattttt tctttcacca tgtatgtgac
    19801 agtagtaatg tatcacacca ttctttttgc aagcctttaa tgcggaatct aaaaactcat
    19861 cagatctctc aggtaaaagc atcagaactc tatcaccttt tcctttgagt ttttcttcta
    19921 taatttttgt agcatcgcct gctatagatt ctacttttcc agcaagcttg tttagcttta
    19981 tgtttttttc acacaatgct gcagcatgtg gatttatgtc aatattatag acggtacatt
    20041 ttttcttctt tgccgcaatt atagaaaaca ttcccacacc accaaacata ttgataactg
    20101 tttcaccatc ttcaattagg tttgcaattc tttctctttc tgtagaaagt cttggagaaa
    20161 agaatgcctt ttggacgtct accttaaacc tgcatccatg ttctttgtat tctgtttcag
    20221 ttctatcttc tcctgcaaga agttctaagt tacgggttcg aaaatctcct tctactggag
    20281 atgattgata aaaaacagat ttggctgtct ttacctgttg caaaagaatt tctccaatta
    20341 tttttttctt ggtaacaagc gaatcgggta tcctaataat tatgatatct ccaatttgat
    20401 caaacgcacc atataattct gaaatttctt tttctgataa tactcctgcc actgcttctt
    20461 ttagcatccg agtcaatttc tatagtaaaa gtgacctgac tgtttttgtt ctctgtcgag
    20521 tctgctttct gttttgttta ctctgggtct aagacttgtc tcatctcgtc tatacgtgat
    20581 atcaagagat cttaagaaac gattcattcc ctgtgcttta ggcataggtg atgtaccatg
    20641 accctctttt cctggaattc cttcaaaaat tactaatgaa tcagaaatta gatccatcaa
    20701 ctgtatatca tgatcaatta tgattgcaga cttgccataa gagcgaacaa atctctgaat
    20761 aagctttgca actgcaattc tatcttctac atctagaaac gctgaaggtt catctaatgc
    20821 atatagatct actttttgta aaagacatgc aacaactgca accttttgta attctcctcc
    20881 cgaaagattc ttgactgatt tgttatagag tttcgttaat cgtaagggat caattacttg
    20941 ttcttcttcc attgtgcctt caagtccgcc accatttgcc ttttccaaaa ccgaaattac
    21001 ttccacatca aagtcatttg tcagatactg tggtttgtat gcaatcttca catttgattc
    21061 aagtttgcca aaatcaggtt tttcaacacc tgcaatcatt ttcatcagag ttgttttacc
    21121 taatgcgttt gcacctacaa caccaagaac ttcgtttttg cgtactttac cagcatcaac
    21181 tgaaacagaa aagttaggat attttctttg taatgctgga tattcaacaa cattatctgt
    21241 cataacaaac tcatctgctg aagttgatgc atcaaatctg aatgcaatgt ctcgaaatct
    21301 aacgttctca tttggtagat acccgtccaa aaatacattg attcctactt tggtagatag
    21361 tacatctgca acaataccat aagcagcagg ttctccatac aacacatcaa taaaatcact
    21421 tagataatca agcacggtca agtcatgttc taccaccatg acactcttgt caagtttagc
    21481 aagactttga attactctgg ccatgcctat tctttgaaat acatcattgt atgaagaggg
    21541 ttcgtcaaaa aagtaaaaat cagaatcttt tgatgcagca actgcaatgg caagtctttg
    21601 taactcacca ccactgagct cattcaattt attatccatg gaattttgta atcctagttc
    21661 tcttacaagt tctggcatga catttcgctc atcgtatttt tctaaaagtt cttttgcagt
    21721 tccatcaaac actttagtta aattgtagac ttgttgtggc tttattgatg ccttgatctg
    21781 ttcatttgca attttttcaa aatgtagttt caattcggta ccttgaaagt tctttaaaat
    21841 atcattccac tctgggggtg atgcataatt tcctaaattt ggttttaggt tgcctgacag
    21901 aatgctaaca attgtacttt ttcccatgcc gtttcgacca agcaatccga ctactctgcc
    21961 tttttttgga gtaggaattc tgtacaatct gaaagaattt attccaaact ggtgaatttt
    22021 ttccgttttt agctcttgtg caagattaac aattgttatt gcctcaaatg ggcatacctt
    22081 tacacaaatg ccacatccgt tgcaaatgtt ctcatctatc tgggcttttc caatctcctc
    22141 atttagtaca atacagtcag ctcctgattt gtttactgga cagtacttga tacactcaag
    22201 accgcatttt tttggctggc ataattcctt atctaatact gctactcgat gagtcataga
    22261 tttacaaaat cattcttcaa tttatacctc ctattcaagt aagattaata cagaagatta
    22321 tcaaaagaac tttcaagccg gccatagtgt tggggtccga cccggtccca ttccgaaccc
    22381 ggaagtcaaa cccaacgtcg ctactgtgtt actaaggtgc gagagccctt gggaagcagt
    22441 agtgctggca cctttctaat ctaatgctac actgtttggc gtagctttcc aaattgcttc
    22501 tactgtgata cttctattca ctccgcgttt ttctgcatca tctggaaaat atccaatgta
    22561 ttcttcaaca agactcttca ttttttcatc aatatcttct acttttgcag catctgtgaa
    22621 aagatatggc atttcattgc acgcaatcat atatccttgt ggatttggtg taattgaaaa
    22681 tgataatttt ggtaaccttg tggttttttg cataatgtat ctatgatctg gaaaccctaa
    22741 aaagatggcg atatgtgaat cactctatgg caagctgtgc agtgtgcggt gagcagttct
    22801 atgatgatat acgattccta aatcacatga tggaaaagca tggctttaga aatcctaaca
    22861 gtaacaatgc tcctgataga aatagtaggg gatactgact attttatcag aatcttttcg
    22921 ctgcttgaaa agagactctt tgttctaaga aagactgcct ttggggtctt gcactctgca
    22981 ctacaagatg cctgtatgtc gttttgagta tgagatctta ttttaccaat caaaatagtc
    23041 gtgatgagag gcaaaagcgc agctcttcct ccatatgcaa ttttaccgtc tataaaccag
    23101 aacattttgg taggatcata cttgtctgaa aagatttctg actttatttc tatgccaaga
    23161 tctgtgtagt gacctactat caacaatttt tttcttgcaa aaagacttac aacttttgca
    23221 aatttgccgc aaagataaca tttgtcgtca tatatcataa taggcacaag actctgaata
    23281 tccatgcttt attgttggtt ttaccaaatt aaaattttct gaaataactt tttatttgct
    23341 ccaaatgcgc taaaaatcat gaatgcggat ttacctttga atactcaatt tcatactttg
    23401 taaagatatg ggactgaatt attataaaat cataaaaagc gttcgcgagg cacgcaaact
    23461 tgtaataaaa gcaaccgcta ctgctggttc tggacatcca ggtggctcat tttccatggc
    23521 agaaatcatg ggatgcatct tccacaaatt cttacgctat gatccaaaga acccattgtg
    23581 ggaagaccgc gacagactag ttttgtcaaa aggacatgca tcaccaggat tgttttcaaa
    23641 tctggcagta gctggatact ttgataaatc agaggttgaa accttgagaa agtttgaaag
    23701 tagattacag ggtcaccctg atttcaaatg tccaggagta gaattttgtg gcggttcact
    23761 gggaattggg ctgtcttttt ccataggaat ggcacttgcc gcaagaattg atcaaaaaca
    23821 gcaccgaata tacaccataa tgggagatgg tgaatcagac gaaggacagg tttgggaagc
    23881 cgcaatgact gcctcaaaat acaaacttga taatctcacg gtatttctag ataggaactt
    23941 catccagcaa gatgactata cggaaaaagt aatgcctctt gatgaggaac taattggtga
    24001 tgatatttca gaaatgtgga aaaatgcagc aagatggaag actggtgaca agtggagagc
    24061 ttttggatgg aatgttttag aaattgatgg acatagagtt gaacaaatca caaaagcggt
    24121 aaaatcagct ttgcaaacaa agggaactcc atcaataatt gtatctcgca cagtcaaggg
    24181 aaaaggagta gaacacatgg aggacaaccc aaaatggcat ggtgttgcac caaacccaaa
    24241 aattgcacca ataattgatc ttgagcttga ctgtcaattt ctgatctctc catcaataat
    24301 tgccggtgat atgactaatc tagaaaatga agtaaaaagg gcaactcatg gtcgtgctga
    24361 ttttatccat cttgatgtaa tggatggaca gtttgttcaa aatactactt ttgatcacaa
    24421 taaaatcaag gaattacgat ctattacatc aattccattt gatgcacatc tgatgattaa
    24481 tgagccagta aaacatgtca gaaattatgt tgaggctgga agtgatataa ttacagtaca
    24541 tgcagaggtt tgtaatgagt caagctttgg agaaattcat gatgtattac gtgcaaacga
    24601 tgtcagtgta ggccttgcaa taaatccaga tactgaactt ccatcttggg ctgaaaaatt
    24661 catccctgat ttggatcagc taattgtaat gtctgttgtg cctggaaaat caggtcaaaa
    24721 gtacattgaa ctaacacatg agaaggtaca aagactctca aaggtactag cagagaagca
    24781 attttctggt tttattgagg cagatggagg ggtagacctt tcaaacgtag gacagtgctt
    24841 tttggatggt gctcgtgtat ttgtaggtgg aagtgccata attggacaaa aagacgttcg
    24901 agccacaatc actgaattta gaaaaaagat actttatgca agaagaaaac tcttgattca
    24961 aaaagcaaac gagcttggtg gagtagatct tgtcaacaaa tggatagatc tgcacgaagt
    25021 tggcaaaaaa aagacagagt tattgcaaat tgcaaaggag gctggattta catgactgaa
    25081 atgacagaca tgcgcacagt atatggagaa actctgatta aagtaggaga agaaaatcca
    25141 aatgttgtag ttgttggcgc tgataccacg gattcattaa agacaaaacc ttttggaaaa
    25201 aaattcccaa acagattctt taatgttgga attgcagaag caaatctagt ttctgttgct
    25261 gcaggtcttg caattgaagg caaagttgca tttgcaagta cgtatgccat atttcttcca
    25321 ggtagatgtg ttgatcaaat aagaaatgca atcgcatatc catcacgtgg agataaaaaa
    25381 ggacttaatg taaaaatggt tgtatcgcac ggtgggttaa gtgtcggggc tgatggtggt
    25441 tcacaccagc aaattgaaga tattgcaatt atgcgaacca tccctaacat gaaagtgata
    25501 attccagctg acacagttac agtttcgaaa ttgacttgga taatgtcaca aacctatggg
    25561 ccgttttaca tgcgaatggc acggtcaaaa actccagttg tacactcgga agcacaagag
    25621 tttgagattg gaaagggaat tgttttgcgg gatggttctg actgcactat tgcagcatgt
    25681 ggaataacag tgccaatggc acttgaggca gcagagactc tcaaacaaga aggtatctca
    25741 tgcagagtat tggatttgtt ctcaatcaaa cccatagatt ctattctcat agaaaaggca
    25801 gcacgtgaga caggaggaat tgtaacatgt gaggagcaca acatattggc tggttttggt
    25861 tctgcagtat ct
//
LOCUS       DATIDL010000057         5254 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrosopumilaceae archaeon isolate SMAG_U16342
            k141_1885652, whole genome shotgun sequence.
ACCESSION   DATIDL010000057 DATIDL010000000
VERSION     DATIDL010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935836
            Sequence Read Archive: SRR25158326
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosopumilaceae archaeon (soil metagenome)
  ORGANISM  Nitrosopumilaceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrosopumilales;
            Nitrosopumilaceae.
REFERENCE   1  (bases 1 to 5254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/04/2024 05:09:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,520
            CDSs (total)                 :: 1,474
            Genes (coding)               :: 1,474
            CDSs (with protein)          :: 1,474
            Genes (RNA)                  :: 46
            rRNAs                        :: 1 (5S)
            complete rRNAs               :: 1 (5S)
            tRNAs                        :: 43
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5254
                     /organism="Nitrosopumilaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1885652"
                     /isolate="SMAG_U16342"
                     /isolation_source="soil"
                     /db_xref="taxon:2202732"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            8..160
                     /locus_tag="VLB45_02530"
     CDS             8..160
                     /locus_tag="VLB45_02530"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HSD04609.1"
                     /translation="MGTYGVYDLFVKVQAQTSRDVEHIITHQIRKIKNVLSTTTLSVI
                     PEQGGK"
     gene            192..563
                     /locus_tag="VLB45_02535"
     CDS             192..563
                     /locus_tag="VLB45_02535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04610.1"
                     /translation="MKIGLVAACIAGSIVVILIAMIYAMQGPATQYSIFLDPFKDPQD
                     LFTTARVTIQNNGNAPLTNVLIDYGGNFEKIPMLKPGQKMILSPPTGSALEKVTVTSD
                     EGIHVTMPYRLPTKMPGMMGS"
     gene            607..1434
                     /gene="purC"
                     /locus_tag="VLB45_02540"
     CDS             607..1434
                     /gene="purC"
                     /locus_tag="VLB45_02540"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HSD04611.1"
                     /translation="MKFLRSGKVKDLYELDDGNLLFHFSDRVSAFDVKFPTLIPRKGE
                     VLCRFAEFWFKELPIKSHYVRTEAKNKLVVKKMSMIPIECIVRGYFYGSLITRWKNGE
                     ITLPKNTDTTMAAKLSEPIFDPTTKSEEHDLPINKKLALEKNLVTAKEFDWLEETSIS
                     IYKKMVTIAEKSGFILADLKLEFGKSENAILLGDSIGPDEYRMWPKSQYQVGKVQESF
                     DKQLLRDWLAEHGYQKQFEDDRNAGKEPVAPELPLELCQQMSQRYVTAYERITGSSL"
     gene            1731..2288
                     /locus_tag="VLB45_02545"
     CDS             1731..2288
                     /locus_tag="VLB45_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HSD04612.1"
                     /translation="MSTLLQKELKVNRIVTTNVGQAKAFDDPARMKILQILYRKQLST
                     EQIVEELRKEGYKKATTTIRHHLDVLKHAGLVEIVKIQEVRGAVMKYYGTSVKILGFT
                     LPSNFDSEYSKTITDASLKMEKIIKNIAQKNTSKIRKKYSENQYGEYVLMEIVNRAVT
                     NVFENQKFILDFVPSKKLKDVSTNN"
     gene            complement(2285..2701)
                     /locus_tag="VLB45_02550"
     CDS             complement(2285..2701)
                     /locus_tag="VLB45_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF371 domain-containing protein"
                     /protein_id="HSD04613.1"
                     /translation="MHFEISFYGHKNIRSLHAKTIEITTESELTLNGDCIIGVKASCG
                     CNNIPKEMKKLLQDSRSNIQFTITVNDLSFKVKGKGHHNLILTHPHDIVIRKSSFVCP
                     RTLAINCNAASDSIPRQMIKTLQDPKTKGTFAIDVT"
     gene            2754..3491
                     /gene="psmA"
                     /locus_tag="VLB45_02555"
     CDS             2754..3491
                     /gene="psmA"
                     /locus_tag="VLB45_02555"
                     /EC_number="3.4.25.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000502 - proteasome complex [Evidence
                     IEA];
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaeal proteasome endopeptidase complex
                     subunit alpha"
                     /protein_id="HSD04614.1"
                     /translation="MLPAAAGYDRAITVFSPDGRLYQVEYAIETVRRGTLAIGIKSKD
                     GIIIAVEEKPRKLQNSEVTQKIFQVDDHIGVAAAGYIPDARSQVDNARFFSQSNRMIY
                     DEPIEVESVAKHLADQCQQFTQYAGVRPFGVALIIAGVDKSGSTIYLTDPSGTYISYD
                     AVAIGSGSEQVNEYLEKYYKKDCTLEDAAILAVESIYLASEDKEGSKHIKMSQITNEA
                     KILTKVSEKDVEKYAAKAKEHSTKRQS"
     gene            3531..4346
                     /locus_tag="VLB45_02560"
     CDS             3531..4346
                     /locus_tag="VLB45_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008300689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative RNA uridine N3 methyltransferase"
                     /protein_id="HSD04615.1"
                     /translation="MKLSIAIPDSALIDESTQLDKSRKISLIARTCAIFKVQTIYIYH
                     ESSGSEQDKSLLRTILKYLETPQYLRRSLFPKISELKFAGILSPLKIPHHTYTSDLSR
                     IKAGDVREGMVFFAKGGKFVDVGLDKPIPYFGEEKEGRRITIKFKTGFPDLSIKQISR
                     GEVSQYWGYEIKESANLRTLLSNWNSDIILTSKKGKIIHKNQKYFEGLSDAEVLVVFG
                     SPERGVHEILGGSIHGIQKSQVFNFFPDQATETVRLEEAMLGALSILNILTRN"
     gene            4568..>5254
                     /gene="rplC"
                     /locus_tag="VLB45_02565"
     CDS             4568..>5254
                     /gene="rplC"
                     /locus_tag="VLB45_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010194288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HSD04616.1"
                     /translation="MGHRKHSQPRRGSLAYLPRGRAKSMEARIRTWPDQEGDQPKLLA
                     HAGFKAGCIRIASIDDRDKTPNFGKQLVSLGTVVVTPPVTVIGIRGYAKDRYGLDSIF
                     DVYAKDLPKELPRLFVAKSDEKSLERAEKSLGRISELFAIVAVLPQQAGREQKKPYVF
                     EVAVKGGDITKQFAYVKDLLGKEVKIDQVFQKGSDVDVAAITKGKGIEGPITRWGVKK
                     KQHKSRKSVRA"
BASE COUNT         1879 a          862 c          990 g         1523 t
ORIGIN      
        1 agaagtcatg ggaacatatg gtgtctatga tctttttgtt aaagttcaag cacagacaag
       61 tagggatgta gaacatataa ttacacacca aattagaaaa ataaaaaatg tcctttcaac
      121 aacgacgctt tctgtaatcc cagaacaggg cggcaaatag cctatataat tggaaagaac
      181 aattaacatc attgaagatt ggtcttgttg ccgcttgtat tgctggctca attgtcgtta
      241 tactgatagc aatgatatat gcaatgcaag gaccggcaac acaatactct atatttttag
      301 atccattcaa agacccacaa gatttgttca ccactgcacg tgttacaata caaaataatg
      361 ggaatgctcc acttacaaat gtgctaatag attatggcgg gaatttcgaa aaaatcccga
      421 tgttgaaacc tggccagaaa atgatacttt ctcctcctac tggaagtgca ttagaaaagg
      481 tcacagtgac ttcagatgaa ggcatacatg tgacaatgcc ttatagattg cctacaaaga
      541 tgcctggcat gatgggttca taatctagaa tcagtttaaa tcacacattg cccaatgacc
      601 gcatgtttga agtttctacg ctctggaaaa gtaaaagact tgtatgagtt ggatgatgga
      661 aatctacttt ttcatttcag tgatcgagtt tctgcatttg atgtaaaatt ccctactctg
      721 attcctagaa aaggcgaagt gctttgtagg tttgctgaat tttggtttaa agaacttcca
      781 atcaagagtc attatgttag aaccgaagca aaaaataaac tggtagttaa aaaaatgagc
      841 atgattccaa tagaatgtat cgttcgagga tatttttatg gcagcttaat aacgcgatgg
      901 aaaaatggtg aaattacatt acctaaaaac accgatacca ctatggctgc aaaactatca
      961 gaaccaattt ttgatcctac tacaaaatct gaagaacatg atctgcctat aaataaaaaa
     1021 cttgcattag aaaaaaatct tgtaaccgca aaggaatttg attggttaga agaaacatca
     1081 atcagcattt acaaaaaaat ggttactata gcagaaaaat caggatttat cttagccgat
     1141 ttaaaactcg aatttggaaa atcagaaaat gccatactgc ttggtgattc tattggtcca
     1201 gatgaataca gaatgtggcc caaaagtcag tatcaagtag gcaaagttca agaaagcttt
     1261 gacaaacagc ttcttcgtga ctggttagct gagcatggat atcaaaaaca attcgaagat
     1321 gatagaaatg caggaaaaga acccgttgca ccagaactcc cgttagaatt atgccaacaa
     1381 atgtcacaaa gatacgttac tgcatacgaa cgcattactg gcagttcact ttaactcgaa
     1441 tcttgatagt aaacaaactg ttggataggt cattgacggc ttattttttc ataaaatgct
     1501 caagatttct tagaatccca tttttttaaa aattttttgg atttattgta acgctacaat
     1561 attgttagtt ctggtatcat gcatatagtt ctggtattgt aatttgttgt aatataatta
     1621 gaatcattga aggcattaca aattttttag tagcataaaa aagacaatcc aattagacaa
     1681 aaaattgtga tatcttgatc ttttagatcg aatcataact cgataataga atgtctactc
     1741 tattacaaaa agagctcaaa gtgaacagga ttgtcactac gaacgtggga caggccaagg
     1801 cctttgatga tcctgcaaga atgaaaattc tacaaatatt ataccgtaag caattgtcca
     1861 cagaacagat cgtagaagaa ctacgtaaag aaggctacaa aaaagccacc actacaatta
     1921 gacatcactt agatgtgcta aagcacgctg gcctagttga aatcgtaaag atacaagaag
     1981 taagaggagc tgtgatgaag tactatggca catcagtaaa gattttagga tttacacttc
     2041 cttcaaactt tgattctgaa tattctaaaa caataactga tgcatcatta aaaatggaaa
     2101 aaataatcaa aaacattgct cagaaaaata cttcaaaaat aagaaagaaa tattctgaaa
     2161 atcaatatgg ggaatatgtg ttgatggaaa tagtaaatcg agctgtcaca aacgtatttg
     2221 aaaaccagaa atttatttta gattttgtac catcaaaaaa actaaaagat gtatctacaa
     2281 ataactaagt cacgtctatt gcaaaggttc cctttgtttt tgggtcttgc agggttttta
     2341 tcatttggcg aggaatagaa tctgatgccg cattacaatt tatagccaaa gttcgtgggc
     2401 agacaaaaga actttttcta attacaatgt catgtggatg tgttaaaatc aaattgtgat
     2461 gaccttttcc ttttactttg aaagaaagat catttacagt aattgtaaat tgaatatttg
     2521 atctagaatc ttgcaaaagt tttttcatct cttttggtat gttgttacaa ccgcagcttg
     2581 cttttacacc aattatgcaa tcgccattca atgtaagttc tgattctgtt gttatctcga
     2641 ttgtttttgc atgaagcgat cgaatatttt tgtgaccata aaatgagatc tcgaaatgca
     2701 tgcgtaaagt atcactagac ttaaattaaa ccttcaaagg tatgaaaatt gaattgttac
     2761 cagcagctgc aggatatgat agagcaatca cagtattttc tcccgacgga agattatatc
     2821 aagtagaata tgctattgaa acagttcgac gcggaacact tgcaattgga attaaaagca
     2881 aagatggaat tattattgca gtggaagaaa aaccacgcaa attacaaaat tctgaagtga
     2941 ctcagaaaat tttccaagtt gatgatcaca ttggcgttgc tgcagcagga tatattcctg
     3001 atgcacgaag tcaggttgac aatgctagat tcttttcaca aagcaacagg atgatttacg
     3061 atgaaccaat tgaggtagaa tcagtagcaa agcatcttgc tgaccagtgt caacagttta
     3121 ctcagtatgc tggtgtacga ccttttggag tagcattgat aattgcagga gtagataaaa
     3181 gcggaagcac catttatctt acggacccga gcggaacgta catctcttat gatgcagtag
     3241 caatcggttc tggttctgaa caagtaaatg aatacttgga aaaatattac aaaaaggatt
     3301 gtacattaga agatgcggca atccttgcgg ttgaatcaat ttatcttgca agcgaagaca
     3361 aagaaggtag taagcatatc aaaatgtcac aaatcacaaa cgaggcaaaa attctaacca
     3421 aagtatctga aaaagatgtt gaaaaatacg cagcaaaagc aaaagaacat tctacaaaaa
     3481 gacaaagctg atctctagca ttttataacg tacaaaatta atctattacg ttgaaactat
     3541 ctatcgcaat tcctgattct gcattaattg atgaatctac acaacttgat aaatccagaa
     3601 agatttcact aattgcaaga acttgtgcca tattcaaagt gcaaacaatt tacatttatc
     3661 atgaaagctc aggaagtgaa caagacaaat cccttttgcg aacaattctg aaatatttag
     3721 aaaccccaca atatttgcgc agatcattgt ttccaaaaat ctcggaatta aagtttgcag
     3781 gaattctcag tccattaaaa attccacatc acacctatac ttctgatcta tctagaatca
     3841 aagccggcga tgtaagagaa ggcatggtat tttttgcaaa aggaggaaaa tttgtggatg
     3901 tcggattgga taaaccaata ccatattttg gagaagaaaa agaaggacgg agaataacca
     3961 tcaaatttaa gactggcttt ccagatcttt caataaaaca aatctcacga ggtgaagttt
     4021 cacagtattg gggttatgag attaaagaaa gtgcaaattt acgcacgctt ttgtctaatt
     4081 ggaattctga tataatactt acatcgaaaa aaggtaaaat cattcacaaa aatcaaaagt
     4141 attttgaagg attatctgat gctgaggtac tagttgtttt tggttctccg gaacgtggtg
     4201 tccatgaaat tcttggtggt tcaattcacg gcattcaaaa atcacaagtc tttaacttct
     4261 ttccagatca ggctactgaa actgttcgcc ttgaagaagc tatgctaggt gctttatcta
     4321 tcttgaatat tcttacgcga aattaatatt gtttataatt ttagaatttt tatgttaaaa
     4381 caaaaacaat ttctgctttt tgcaattgct gtaggtatta cgggaattat tattggtgga
     4441 ataatattcg caagcacaat tgtagatttc ttatatccat aagatcacaa attgcataaa
     4501 aattagatca aattgttatt tctgatagta gacttataat ggggtttacg acatttagcg
     4561 tttatccatg ggtcatagaa agcatagtca gccaaggcga ggaagtcttg catatcttcc
     4621 aagaggacga gcgaaaagca tggaagcgag aattcgtacg tggcctgatc aagaaggcga
     4681 tcaaccaaaa ctattggcac atgctggttt caaggcaggt tgcattagga tcgcaagcat
     4741 agatgacaga gacaagacac ctaactttgg aaaacaactg gttagtcttg gtacagtagt
     4801 tgtgactcct cctgtcaccg taattggaat acgtggttat gcaaaagaca gatacggttt
     4861 agattctatt tttgatgttt atgcaaaaga tttaccaaaa gaactaccaa gactatttgt
     4921 agcaaaatca gatgaaaaga gtcttgaaag agcagaaaaa tctcttgggc gtatctcaga
     4981 actctttgct atagtggcgg tgctcccaca acaagctggt agggagcaaa aaaaaccata
     5041 tgtttttgaa gttgctgtaa aaggtggcga tattacaaaa caattcgcat atgtaaaaga
     5101 tctcttaggt aaggaagtta aaattgatca agtttttcaa aagggctctg atgtcgatgt
     5161 tgctgccata acaaaaggaa aaggaattga aggaccaatc acaagatggg gtgttaaaaa
     5221 gaagcaacac aaatcgagaa aaagtgtaag agct
//
LOCUS       DATIDL010000058         4003 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrosopumilaceae archaeon isolate SMAG_U16342
            k141_877337, whole genome shotgun sequence.
ACCESSION   DATIDL010000058 DATIDL010000000
VERSION     DATIDL010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935836
            Sequence Read Archive: SRR25158326
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosopumilaceae archaeon (soil metagenome)
  ORGANISM  Nitrosopumilaceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrosopumilales;
            Nitrosopumilaceae.
REFERENCE   1  (bases 1 to 4003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/04/2024 05:09:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,520
            CDSs (total)                 :: 1,474
            Genes (coding)               :: 1,474
            CDSs (with protein)          :: 1,474
            Genes (RNA)                  :: 46
            rRNAs                        :: 1 (5S)
            complete rRNAs               :: 1 (5S)
            tRNAs                        :: 43
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4003
                     /organism="Nitrosopumilaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_877337"
                     /isolate="SMAG_U16342"
                     /isolation_source="soil"
                     /db_xref="taxon:2202732"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..549)
                     /locus_tag="VLB45_02570"
     CDS             complement(<1..549)
                     /locus_tag="VLB45_02570"
                     /inference="COORDINATES: protein motif:HMM:NF016166.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF432 domain-containing protein"
                     /protein_id="HSD04617.1"
                     /translation="MKVTEDSFSTENTDSVNNTLGYSKYGHYIVKKELEINLPNTAIK
                     FSHHSKNKFSYHRTNSESEDVHKIIAVKSEPLQIELIPSLPLHLPAYKTDFVFLRFIN
                     KLFLSANSSTEVVVPFPIELAICITENNTTDYLDFFTCDGSNSRYGLYGSPEEGKLCK
                     YARIPWEYNENVSYFRYAKMKIT"
     gene            810..2270
                     /gene="glnA"
                     /locus_tag="VLB45_02575"
     CDS             810..2270
                     /gene="glnA"
                     /locus_tag="VLB45_02575"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HSD04618.1"
                     /translation="MTASSGQSENIIKKIHDDKIDFIDFWFVDIFGGLHSVGIPAYAL
                     TEEHFKNGLEKLDASSIRGFKSVNKSDMVMVPDTSTFRILPPDYDEGNRKNARMFVDL
                     YEKNVSSHLRYNRDSRGIVSGAIKELNVFGLTHARWGPEVEFFIFDKINLFPSSIGAI
                     QSYGGSGYSIESKEAPWTRGNASTRIDLKEGYYPAQPRDTLDGIRKDICDDLYQYFGI
                     KVEAQHHEVATSGQCEINLVNDDTIPMADSIVTIKNLVKVKSKSKGKVATFMPKPIYG
                     DNASAMHVHQSLWFNEKNIMYDPDDKVAELSQVGRYYVGGILEHASAICAISNPTTNS
                     YKRLIPDFEAPVSVCWGISNRSSAIRIPMYSRGNEKSKRIEYRVPDPTANIYLLEAVL
                     LLAGLDGIRKKIDPGNPIEENIYKLTPEQKRSYKIASLPVNLEEALDALQSDSKFLEH
                     VFTKDFLEIYSAMKYAEYAAFAQTPTAWEVSMYADV"
     gene            complement(2267..3634)
                     /locus_tag="VLB45_02580"
     CDS             complement(2267..3634)
                     /locus_tag="VLB45_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HSD04619.1"
                     /translation="MSSHTITDSVRSFFDSFNVTEQAQNGIPDSNFLKENISNLQQSL
                     SQSVFQNVDLEAAQVVLLAIGVIIFLGIAGESFFRRTGIPDVAFLMIFGAVLGPILGI
                     VDTDSVVKIVPYFAALALIIIMFDGGLNLNIKYVIKTAHFALLLSVAGFAISVIFVTL
                     IAVYGLGWQLLDSILLGSIVGGSSSIIVFGLVRRLAITDETKSMLSLESAITDILATI
                     AAFLLFETVMSGHFDPNLVGVTAGKAVGVGLILGFGVGIPWMYVSTKIANAKHAYMFT
                     LAMLFVLFFLAKSFGESGALTALIFGLMIGNKQLLSPYLKFKLPQVKTDDSFHEELTF
                     LVRSFFFVFVGLLASFGQLEYLIFGVIMAVAIYIGRIGIVKASLTKRFPEFDKKVTAV
                     MLPRGLAAAVLATLPLTLGLPNAEAYPQIVFVIIMSTVIITTLGLTRASKGQPKHIEP
                     EKRDN"
     gene            3774..>4003
                     /locus_tag="VLB45_02585"
     CDS             3774..>4003
                     /locus_tag="VLB45_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HSD04620.1"
                     /translation="MAIESHFIQTIIGVGILLFAAKLMAELFHRLKLPIVLGELLAGM
                     IVGPFALGAFLVYDGQPLLKIDEELKILGEIGA"
BASE COUNT         1362 a          699 c          701 g         1241 t
ORIGIN      
        1 ggtgattttc atcttagcat atcgaaaata tgagacattt tcattatatt cccaaggtat
       61 tctggcatat ttgcaaagct tgccttcttc aggtgatccg tataaaccgt aacgtgaatt
      121 acttccatca catgtaaaaa aatctagata atctgtcgta ttattctctg taatacatat
      181 tgctaattct ataggaaacg gaacaacaac ctcagttgaa gaatttgcac tcaaaaatag
      241 tttgtttata aaacgtaaga acacaaaatc tgttttgtat gcaggtaaat gaagtggtag
      301 agacggaatc agttcgatct gcaatggttc agattttact gcaattattt tatgtacatc
      361 ttcactttcg gaatttgttc tgtgatatga gaacttgttt ttactatgat gtgaaaattt
      421 aattgctgta ttgggtaaat taatctcaag ttccttctta actatgtaat gaccatattt
      481 agaatatcct aaagtgttat ttacagaatc agtattttct gtagaaaatg aatcttcagt
      541 tactttcatt tcataattag tgttttcatt atatttattt agttctaatt ttttttctta
      601 atttttattt tgagcaacac ttaacaatga tgaactatat tatttcatat gaggttacaa
      661 caaattgaaa atgaatggaa caaagcaagc ttatttctgc acaaatcata ttcactaaat
      721 ttttgtgata ttcttcaaaa agatattaca tcattaggaa cagaagtaat cttagtgagc
      781 agtggttcac cataggtgta acatctgagt tgactgcctc atcagggcaa tctgaaaaca
      841 ttataaaaaa aattcatgat gataaaatcg atttcattga tttctggttt gtagatattt
      901 ttggtgggtt acatagtgta ggtattcctg catatgcatt gactgaggag catttcaaaa
      961 atggtctaga aaaattagat gctagctcaa ttcgtggctt caaatcagtt aacaaatcag
     1021 atatggtgat ggtgccagat acatctacat ttagaatact cccgccagac tatgatgaag
     1081 gaaatagaaa aaacgcacga atgtttgtag acctatatga aaaaaatgtt tcttcacact
     1141 taagatataa cagagattcg cgtggaattg tctctggcgc cataaaagaa ttaaatgtat
     1201 ttggattaac tcatgcacga tggggacctg aggtcgagtt tttcatattt gacaaaatta
     1261 acttgttccc atcttcaata ggtgcaatac aatcctatgg tggctctgga tattctatag
     1321 aatcaaaaga agctccgtgg actagaggta atgccagcac aagaattgat cttaaagaag
     1381 gatactatcc cgcacaaccg cgagacacac ttgatggtat aagaaaagac atctgtgatg
     1441 atctgtacca atattttgga ataaaagtgg aggctcaaca ccatgaagta gcaacatcag
     1501 gacaatgtga aattaatcta gttaacgatg acacaattcc tatggcagat tcaattgtaa
     1561 caataaaaaa tttagtaaaa gtaaaatcaa aaagcaaagg caaagtagca acatttatgc
     1621 caaaaccaat ttacggtgat aatgcgtcag ccatgcatgt tcatcagagc ttgtggttta
     1681 atgagaaaaa tattatgtat gatccagatg acaaagttgc agaactaagt caggtgggaa
     1741 gatactatgt tgggggaata cttgaacatg cctcagcaat atgtgcaata tccaatccaa
     1801 ctacaaattc atacaaaaga ctaattccag attttgaggc tccagtaagt gtgtgttggg
     1861 gaataagcaa tagatcatct gccattagaa ttccaatgta ttcaagaggt aatgaaaaaa
     1921 gtaaaagaat agaatatcgc gttccagatc ctactgcaaa tatatacttg ttagaggccg
     1981 tattgctttt agctggactt gatgggataa gaaaaaaaat agatcctggt aatccaattg
     2041 aagaaaacat ctacaaacta actccggaac agaagagatc ttacaaaatt gcctccttac
     2101 cagtaaatct cgaagaagcc ctagatgcat tacaaagtga ctctaagttt ctagaacatg
     2161 tatttacaaa agattttctt gaaatctatt ccgctatgaa atatgcagaa tatgcagcat
     2221 ttgctcagac tcccacagct tgggaagtct ctatgtatgc agatgtctaa ttatcacgct
     2281 tttctggctc tatgtgttta ggttgtcctt ttgaggctct agtcaatcct agtgtagtaa
     2341 taattactgt ggacattatg atgacaaata ctatttgtgg atatgcttct gcatttggaa
     2401 gtcccaacgt tagcggtaaa gtcgctagca ctgctgcagc aagtccacgt gggagcatta
     2461 ctgcagttac ttttttatca aattctggaa acctctttgt aagcgatgcc ttcactattc
     2521 caattctacc gatgtaaatt gcaactgcca ttattacccc aaatatcaaa tactctagtt
     2581 gtccaaagct tgcaagtaat cctacgaaga caaagaaaaa cgatctcacc aaaaatgtca
     2641 attcttcatg gaacgagtcg tctgttttta cttgaggaag cttgaatttt aaatacggcg
     2701 ataacaactg cttatttcct atcattaatc caaaaattaa tgctgttagt gcacctgatt
     2761 ctccaaatga ttttgctaaa aagaacaata cgaacagcat tgcaagtgta aacatgtacg
     2821 cgtgttttgc atttgcaatc tttgtagata catacatcca tggaattcca acaccaaagc
     2881 caagaatcaa accaactcca actgccttac cagctgtgac tcctactaga tttggatcaa
     2941 aatgacctga cataactgtc tcaaagagaa gaaaagcagc tatcgtcgct agtatgtcag
     3001 ttatagcaga ttctaagctc agcattgact ttgtttcatc tgttatggca agtcttctta
     3061 ctaagccaaa aacgataatt gagctgcttc caccaactat cgagccaagc aatatgctat
     3121 caagcaactg ccaacctaaa ccatatactg caatcaaagt aacaaagatt actgatattg
     3181 caaaaccagc taccgaaagt aaaagtgcaa aatgtgcagt tttgattaca tattttatgt
     3241 tcaagttaag acccccatca aacattatta tgatcaaagc caacgctgca aaatatggga
     3301 caattttgac tacggaatca gtatcaacta ttcccaaaat tggaccaaga acagcaccaa
     3361 agatcattag aaatgcaacg tcggggatac cggttcttcg gaaaaatgat tctcctgcta
     3421 ttccaagaaa aattatgact cctatagcta aaagtaccac ctgagctgct tccaaatcaa
     3481 cattttgaaa cactgattga gacagagact gttgcaaatt tgaaatattc tcttttagaa
     3541 aattagaatc aggaattcca ttttgtgctt gttctgttac attaaaactg tcaaaaaaag
     3601 aacgtacgga atccgtaatt gtatgagacg acaatcacta tacaatttca tatgacaata
     3661 attaacgtta ggaattaaaa aatttctaat ttggtgtaat ttttctacca tagttttatc
     3721 attggtttct ttttttcaat atttttatac tctattacca tgattgaatc agattggcaa
     3781 tagaatcgca ttttattcaa actataattg gcgtagggat tctgcttttt gcagcaaaac
     3841 taatggctga gctgtttcac agactgaaac ttccaatcgt attaggcgag cttttggcag
     3901 gcatgatagt gggaccattt gcacttggtg cgtttcttgt ttatgatggt caaccactgt
     3961 tgaaaataga tgaagagcta aaaattttag gagaaattgg agc
//
LOCUS       DATIDL010000059         5314 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrosopumilaceae archaeon isolate SMAG_U16342
            k141_2291073, whole genome shotgun sequence.
ACCESSION   DATIDL010000059 DATIDL010000000
VERSION     DATIDL010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935836
            Sequence Read Archive: SRR25158326
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosopumilaceae archaeon (soil metagenome)
  ORGANISM  Nitrosopumilaceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrosopumilales;
            Nitrosopumilaceae.
REFERENCE   1  (bases 1 to 5314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5314)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/04/2024 05:09:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,520
            CDSs (total)                 :: 1,474
            Genes (coding)               :: 1,474
            CDSs (with protein)          :: 1,474
            Genes (RNA)                  :: 46
            rRNAs                        :: 1 (5S)
            complete rRNAs               :: 1 (5S)
            tRNAs                        :: 43
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5314
                     /organism="Nitrosopumilaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2291073"
                     /isolate="SMAG_U16342"
                     /isolation_source="soil"
                     /db_xref="taxon:2202732"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2390)
                     /locus_tag="VLB45_02590"
     CDS             complement(<1..2390)
                     /locus_tag="VLB45_02590"
                     /inference="COORDINATES: protein motif:HMM:NF014108.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HSD04621.1"
                     /translation="MQYKFYTLTLAFLLLFSALPNVMAQSAIDRDKDQIPDNVDDCPS
                     LPEDYEGIMDEDGCPDLYMANLDTDKDGVSDLYDQCPDARETHNFHMDDDGCPDVSPS
                     DKKKGLLDTDNDGITDKLDNCPSQPETYNRFLDNDGCPDQIDSQRDTDRDGLKDSVDQ
                     CPSYAETYNKFIDEDGCPDRAITPSKSSLMVDTDKDGVPDWQDRCRTDAETFNQFKDN
                     DGCPDTIIPDREYIPGSADVDKDGFLDNEDVCPASPETWNKYKDGDGCPDLYPIETLR
                     IGDDTYHVDEQSKVILVGEGSDPNPEDTLSFSWKQTEGEPVKLSSSTVSSPTFMAPAV
                     DNGKTKSLAFELTVDDGVGGKDTDTVEITVDPVNAFPTADAGSDQSVDARTEVTLAGS
                     GEDPDGDRLSFSWKQTAGMPVALSSNSVPSPTFNSPSNANNQPLTFELTVSDGYGGRA
                     SDSVTIFVGKGSVSLLVANAGPDQTVGEGDAVDLDGTKSSAPNDEPLAFMWTQTFGEP
                     VLLDGDTTATPSFVAPDVANGETKTLIFKLTVSSDGLGSATDLVTIKVTTGNGAPTAD
                     AGPDQTVNKRAMVKLAGSGADPDGDKLRYSWKQTDGETVKLSSSIISGPTFNAPNVVN
                     GESKTLTFTLTVSDRYGASDSDSVTITVRPVNNNPTANAGSDKVVDEQTTVALSGSGK
                     DPNKDPLSFSWAQIGGAPVELSDSTTSSPSFTAPIVANGETKTLTFRLTVSDGLGGRA
                     TDSVTVKVNPVNEMPVAKAGSDQTVPENSAARLAGSGSDADGDEITFKWSQLAGPSV"
     gene            2629..4398
                     /locus_tag="VLB45_02595"
     CDS             2629..4398
                     /locus_tag="VLB45_02595"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HSD04622.1"
                     /translation="MPEFAVGEWNLKELVKDPKSPGFTRQLQNIETKVRKFEKNKNHL
                     NPNISTSRFLKTLNELEDISKTVSIAIGYASLLYSANTQSDEATSLLSKMTKLGSEIE
                     NRTLFFDQWWKKHLDQKNATRLTKSAGELSEYLRHKRLIAKYSLTEPEERIINTLDVT
                     GSTALVKLYDKITNSFQFVVKTEGKKKKYTREQLTLLVRHHKPKIREIAYQSLFTEYE
                     KNKGVLGEIYQNLVLNWKNECLEIRKYASPISVRNIGNDVDDKTVNSLLSVCKNNSKV
                     FQKYFSHKASMLGMKKLRRYDIYAPVVTKIKQRKYTFDQAVKLVLKTLNEFSPKLSEH
                     AKKVFVQKHVDSSIRIGKRSGAFCSTIAPEITPYVLVNFNGRSNDVFTLAHELGHAIH
                     SESASDKSIFVQEAPLPLAETASTFSEMLLFDEILQRVSDEEKKSILAEHIDDLYSTV
                     MRQAFFTSFEISVHKKIGDGATVEEITQMYTKNLHEQFSNTITIPEAFGIEWSCIPHF
                     YHSPFYCYAYSFGNLLALSFFQRYKNEGRSFVPNYLEILAAGGSKKPESLLEEHGIDI
                     TSQKFWQDGFRYIENQVERLGKI"
     gene            4553..4708
                     /locus_tag="VLB45_02600"
     CDS             4553..4708
                     /locus_tag="VLB45_02600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04623.1"
                     /translation="MRFPRCCNKEPVYLITYDCGPEPNETMLVCKDHYAEEPFQRFAI
                     KIEKLQE"
     gene            4740..>5314
                     /locus_tag="VLB45_02605"
     CDS             4740..>5314
                     /locus_tag="VLB45_02605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04624.1"
                     /translation="MQKAKLVFGILTIFLFFGGFAAYGQEARLATFHETAQVIIDERF
                     QHQSSSSISIISTSAQEIRVPIDLDQMIHNATNVVAVIITNEDQCVLGVQDSTCVMIN
                     ISREGFEGGINEAQSKGREIGDSLITDINKAFNLDTKFHSVFIHIDDKINKELETSGV
                     VSGKGIVSAVYTMTKQDTSYLFEGLSTILIPS"
BASE COUNT         1668 a         1016 c         1020 g         1610 t
ORIGIN      
        1 actgatggtc ctgcaagctg tgaccacttg aatgtgattt catcaccgtc tgcatcagag
       61 ccagaacctg ctagtcttgc agccgagttt tctggtactg tttggtcaga gcccgctttt
      121 gctacaggca tctcgttaac tggattaact ttaactgtta cagaatctgt tgccctgcca
      181 cctagtccgt cagagactgt tagtctgaat gtcagagtct ttgtctcgcc atttgctact
      241 attggtgcag taaatgatgg acttgatgtt gtgctatctg atagttcaac aggtgcaccg
      301 ccaatctgtg cccatgagaa tgacaatgga tctttgtttg gatcctttcc agagccagat
      361 agtgctactg tggtttgttc atctaccact ttgtcagagc cagcattagc agtcgggttg
      421 ttgtttactg gtcttactgt gattgttaca gagtcagaat cagaagcgcc atatctgtca
      481 ctaactgtta gagtaaatgt caaagtcttt gactcgccat ttacaacatt tggtgcatta
      541 aatgtaggtc cagagataat tgatgatgat agcttgactg tttcgccatc agtctgtttc
      601 catgaatatc ttaatttatc accgtctggg tctgcaccag agcctgctag ctttaccatt
      661 gcacgtttgt taactgtttg atcaggacct gcgtcagcag ttggggcacc attaccagtt
      721 gttactttga ttgtaactag gtcagttgca ctacccaagc catcagagga tactgtaagt
      781 ttgaatatca aagtctttgt ctcgccgttt gcaacatcag gagcaacaaa tgagggagtt
      841 gcagtagtat caccgtctag tagaactggt tctccaaatg tttgtgtcca catgaatgct
      901 agtggttcat catttggtgc actgctctta gtgccatcca aatctaccgc atctccttcg
      961 ccaacagttt ggtctggacc agcattggct acaagtaggc tgacagagcc cttacctaca
     1021 aaaattgtaa ctgaatcaga tgccctgcct ccatatccat cagagactgt gagctcaaag
     1081 gttagaggtt ggttatttgc attagatggg gaattaaatg ttggagatgg tacactgtta
     1141 gatgataatg caactggcat gccggctgtc tgcttccatg aaaatgacaa tctgtcaccg
     1201 tctgggtctt ctccagaacc tgcaagtgtt acttcagttc ttgcatcaac tgattgatca
     1261 gaacctgcgt cagcagttgg aaatgcattg actggatcta ctgtaatttc tacagtgtca
     1321 gtatccttac caccaacacc gtcgtctact gtaagctcaa atgccagaga ctttgtttta
     1381 ccattgtcaa ctgcaggtgc cataaatgtc ggacttgata ctgtgcttga cgatagtttt
     1441 actggttcac cttcagtttg tttccatgaa aatgataatg tatcttctgg gtttggatca
     1501 cttccttcac ctactaaaat aacttttgat tgttcgtcaa catgataagt atcatcgcca
     1561 attcgtaatg tttctattgg ataaagatct ggacaaccat caccatcttt gtatttgttc
     1621 caagtctctg gacttgcagg acatacatct tcattgtcaa gaaatccgtc cttgtctaca
     1681 tctgcacttc ctggtatgta ttcacgatca ggaattattg tatctgggca tccatcgttg
     1741 tctttgaatt ggttaaaggt ttcagcatca gttctgcatc tgtcttgcca atcaggaacg
     1801 ccatctttgt cggtatcaac catcaaggaa ctctttgatg gagttatggc cctatcagga
     1861 cagccatctt cgtctatgaa tttattataa gtttcagcat aacttggaca ttggtcaacg
     1921 gaatctttga gtccatctct atcagtatca cgttgtgaat caatttgatc tggacagcca
     1981 tcattatcta ggaatctgtt atatgtttct ggttgacttg gacaattatc caatttatcg
     2041 gtgattccat cattgtcagt atcaagcagt cctttctttt tgtcagaagg tgagacgtct
     2101 ggacagccat catcgtccat atggaaattg tgtgtttctc gtgcatcagg gcattggtca
     2161 tacaaatcag aaacaccatc tttatccgta tcaagatttg ccatgtacaa atctggacaa
     2221 ccatcctcat ccatgattcc ctcatagtct tctggtaaag aaggacaatc atcaacatta
     2281 tctggaattt gatccttatc tctgtcaata gcactttgtg ccattacgtt tggcagggca
     2341 ctaaaaagca gcaaaaatgc taaagtgaga gtgtaaaact tgtattgcaa tattaggtga
     2401 ggtaaatcac actgattaaa agctttcgtt cattttgatc acaattttta gatcgtgttt
     2461 tttgttttgc cgaattgatc atttgtagtt tttgctagta ccacaagtaa aaaatcatgg
     2521 catttctact tttctggcag aaccgtgtac cgtatagtag atcgtttttt gaaaatttat
     2581 tcaaagtcca catatggttt ttaacaacca aaatacctct aaagtaaaat gccagaattt
     2641 gcagttggag aatggaattt aaaagaactt gtaaaggatc ctaaaagtcc agggtttaca
     2701 agacaactcc agaacataga aactaaagta agaaagtttg aaaaaaataa aaatcatcta
     2761 aacccaaaca tttctacatc aagatttcta aagacattaa atgaactgga agatatttca
     2821 aagacagtca gtattgcgat aggatatgcc tcacttttgt attctgctaa cacccaatca
     2881 gatgaggcta catcgttgct atcaaaaatg acaaagttag gttctgagat tgagaatcgt
     2941 acattgtttt ttgaccagtg gtggaaaaaa cacctagatc aaaaaaacgc tacaagactg
     3001 acaaagtctg caggagaact atcagaatac ttgagacata aaagactgat tgcaaagtat
     3061 tctctcacag agcctgaaga gaggataata aacacgctgg atgtcacagg cagtactgca
     3121 cttgtaaaac tctacgacaa gattacaaac agcttccagt ttgttgtcaa aacagaagga
     3181 aagaaaaaaa aatacaccag agaacagctt acactactag taagacacca caagccaaag
     3241 ataagagaga tcgcatacca atcacttttt acagaatatg aaaaaaacaa gggcgtactt
     3301 ggtgaaatat atcagaattt agttttgaat tggaaaaacg aatgtcttga aattagaaaa
     3361 tatgcatcac caatttcagt tcgaaatatt ggaaacgatg tggacgataa aacggtgaat
     3421 tcgcttcttt cagtttgtaa aaataattcc aaagtgtttc agaaatattt ttcccataaa
     3481 gctagtatgt tggggatgaa aaaactacgc aggtatgaca tttatgcacc agttgtaaca
     3541 aaaataaaac aaagaaaata cacgtttgat caagcagtaa aacttgtttt gaaaactcta
     3601 aatgagttca gcccgaaatt atcagaacat gcaaaaaaag tattcgtaca aaaacatgtt
     3661 gactcgtcaa ttagaattgg aaaaagaagc ggagcgtttt gcagtaccat tgcaccggaa
     3721 attacacctt atgtgcttgt taatttcaat ggcagatcaa acgatgtttt tactttggca
     3781 cacgaattag gtcatgccat acatagtgaa tccgcttctg acaaatccat attcgtacaa
     3841 gaagctccac tacctctggc agaaacagca tccactttct cagagatgtt attgtttgat
     3901 gaaattttac agagagtttc agatgaagaa aaaaaatcaa tactggcaga acatattgat
     3961 gacctttatt ctaccgtgat gagacaggcg ttttttacta gttttgagat ttccgttcac
     4021 aaaaaaattg gtgatggtgc aactgtagag gaaatcactc aaatgtacac aaagaacctg
     4081 cacgaacaat tttctaacac catcaccatt cctgaggctt ttggaataga atggagttgt
     4141 atccctcact tttatcacag tccattttac tgctatgcgt attcctttgg caatttgctt
     4201 gcactctcat tctttcagag atacaaaaat gaaggaagat catttgttcc aaattatctg
     4261 gaaattcttg ctgcaggagg gtccaaaaaa ccagaatctc tgcttgaaga acacggaata
     4321 gacattacct cacaaaagtt ttggcaagac ggttttcgct atattgaaaa tcaagttgag
     4381 agacttggaa agatttagta ttaaaagcgc ctagcagtca agcgccccca actgctaggc
     4441 aatgttcccc tacggtttga accgatgtca acagtataat ttacagatta caatttaaac
     4501 tattggaaac aagactaaaa ctgttttatt ttatcgtctg taataggaat aagtgagatt
     4561 tccaagatgt tgcaataaag aaccagtata tttgattact tatgactgtg gaccagaacc
     4621 aaatgaaacg atgttagttt gtaaagatca ttatgctgaa gagccttttc agcgatttgc
     4681 aatcaaaata gaaaaacttc aagagtagac ttaataatta cacaagaaaa gttgcatcga
     4741 tgcaaaaagc aaaactggtt tttggaattc ttacaatatt tcttttcttt ggtggatttg
     4801 ctgcatatgg tcaagaagct agacttgcaa catttcatga aacagctcaa gtgataattg
     4861 atgaaagatt tcagcatcaa tcgtcaagtt ccatatctat tattagcact agtgcacaag
     4921 agatacgtgt tccaatagac cttgaccaaa tgatacataa tgcaactaat gttgttgcag
     4981 taataataac aaacgaggat caatgtgttt tgggtgtgca agacagtacg tgtgtcatga
     5041 taaatatttc aagagaagga tttgaaggcg gtataaacga agcacaatcc aaaggacgag
     5101 aaattggaga ttcgttaatt acagacataa acaaggcgtt taacttagat acaaaatttc
     5161 attccgtttt catacatatt gatgataaga taaacaaaga actcgaaaca tccggagtag
     5221 tttctggaaa aggaattgtt tctgcagtat acaccatgac aaaacaagac acgtcgtatc
     5281 tttttgaagg attgtctact atacttattc catc
//
LOCUS       DATIDL010000060        14280 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrosopumilaceae archaeon isolate SMAG_U16342
            k141_1080440, whole genome shotgun sequence.
ACCESSION   DATIDL010000060 DATIDL010000000
VERSION     DATIDL010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935836
            Sequence Read Archive: SRR25158326
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosopumilaceae archaeon (soil metagenome)
  ORGANISM  Nitrosopumilaceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrosopumilales;
            Nitrosopumilaceae.
REFERENCE   1  (bases 1 to 14280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14280)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/04/2024 05:09:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,520
            CDSs (total)                 :: 1,474
            Genes (coding)               :: 1,474
            CDSs (with protein)          :: 1,474
            Genes (RNA)                  :: 46
            rRNAs                        :: 1 (5S)
            complete rRNAs               :: 1 (5S)
            tRNAs                        :: 43
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14280
                     /organism="Nitrosopumilaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1080440"
                     /isolate="SMAG_U16342"
                     /isolation_source="soil"
                     /db_xref="taxon:2202732"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..540
                     /locus_tag="VLB45_02610"
     CDS             <1..540
                     /locus_tag="VLB45_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007551692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HSD04625.1"
                     /translation="TRTLIWTAGVTPVDTIKKSIFKTSRGGVIVNEFLEVPEFPGVFA
                     VGDCCYALNPQTQKPYPPTAQNAEAQAKIAAHNLHALIKKDQKKKFEYSSKGQMAVIG
                     KKSGIATIFGINLHGFLAWWVWRTIYLSKIPRLDKRIRILLDWTVDLFFDRDIARLKI
                     IRREAPKDYKELDEVDDVW"
     gene            complement(547..1806)
                     /locus_tag="VLB45_02615"
     CDS             complement(547..1806)
                     /locus_tag="VLB45_02615"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HSD04626.1"
                     /translation="MNFKIFAFGVSFLLLVNLVPTSFSDSKVAVIETKFGNMVIEFFP
                     NDAPNTVDNFIKLAESGFYDGTKFHRIIPGFMIQGGDPLSKDSRLIQQWGTGEAGYTI
                     KAEFNNIQHKRGIISMARAADPDSASSQFFIVHKDSPHLDGKYTAFGRLVTQESFDVL
                     DKIASLETVHDIASGIPVNIPLQYADAEIYSMKIKNMSEISNILNLGEPERIVTHYTL
                     DEEGNYYNTLFGFSFKAPEGWFVQEPAKTQPEAPDVVVVGPVIDEFTPVVSFLVENSN
                     GTSLDTHITQTKKDLQPLIDAGRLQIISEENKKIKNYDAHITEARGGFVAKDKIFIIK
                     YQEIVFEINDKFYTLTYTNQEKNFDSYIGTFNDIMDSFEPTSTQKNKPVNFPYEYGIA
                     IGIAVAAVAAIFVIKRKKKPLQKNNLP"
     gene            1886..2374
                     /locus_tag="VLB45_02620"
     CDS             1886..2374
                     /locus_tag="VLB45_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04627.1"
                     /translation="MDSDEHIQTHLVSVWGEEKKFLKRGAEGMLLLTEKHVMFVTKTE
                     AKMKWWTTATDRQLRTLLKSNNIMIHQDGYNEEDLRRDLENDANLEFSFDRILNVDFE
                     EKPWAPILKLEINMGDKTKKYHFAIVGGWVKYPLKDPVKYVKVDWAPFVEYIKSRQKI
                     TE"
     gene            2378..3085
                     /gene="cbiE"
                     /locus_tag="VLB45_02625"
     CDS             2378..3085
                     /gene="cbiE"
                     /locus_tag="VLB45_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008297164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046025 - precorrin-6Y
                     C5,15-methyltransferase (decarboxylating) activity
                     [Evidence IEA];
                     GO_process: GO:0046140 - corrin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-6y C5,15-methyltransferase
                     (decarboxylating) subunit CbiE"
                     /protein_id="HSD04628.1"
                     /translation="MGEVYAVGVGPGSPRYVTDAVKEVILYSDVVIGYKYTLKTIERL
                     LSNKQVFEITMQNQEEVYQIVAKTLGDKTCVIPFTGDVNFSESEVVDRLIEIFGDVQI
                     IPGISSIQVAAAKARIPLDKSKVITMHVTTSIEEKKLELQKALLDGYSVILVPRPWPK
                     DPSKHFMQSEIAFYLKNHGFDTSKIKVHVFEFLTTDKETSFVGMLKELEGRQFSDLSV
                     MVFDQTKLDSYINFKTN"
     gene            complement(3125..3970)
                     /locus_tag="VLB45_02630"
     CDS             complement(3125..3970)
                     /locus_tag="VLB45_02630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04629.1"
                     /translation="MKLIALLLISITGIVLASSVQQANSELLPNGIYLLQGNGFIITE
                     KIIEDSELDFQFSTGSKTSSRIKITIEDGVTSISNDEYLLSDGWTGTILREGRFLTLA
                     GNAENLDGDEISLRLTGRLVENSEEGSIYTLTGRLTKDDEVMKVVYNVKIVGTNVVLP
                     TEEPEKETKTIRINILAGASNPGNINYYSIDTVEIAPGTTIIWKNEDSVSHRIMSGVA
                     SFSPGKPFKPDGKIDSGDIAPGQTFSVTIDNLGITRFFDTTYYWMDGVIISLPETKSF
                     STKSQ"
     gene            complement(4027..4761)
                     /locus_tag="VLB45_02635"
     CDS             complement(4027..4761)
                     /locus_tag="VLB45_02635"
                     /inference="COORDINATES: protein motif:HMM:NF021287.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAC2 family protein"
                     /protein_id="HSD04630.1"
                     /translation="MLGLKIHELPKLTNPVMVAALPDMGNVAGIGMDFLVKKLKAKLF
                     AELYAYWPPAVSYDAGLIKYTQSSYKFYYSKKENLIIFSGEFNPSDPRRLYEICYEVI
                     DMAKKLKVTTLYSIGAALRQPDPTAPKIFGATTSPTLLNQLKKQKMNLLDGKGQITGF
                     NGLILGIAQEKNIDSVCILGEIDNPNVIQPKTAQMILSSLLEFLKIPPFDMKELEEEE
                     KKKKFMEQQMNYMDGLMRRDKQPGIA"
     gene            complement(4798..5334)
                     /locus_tag="VLB45_02640"
     CDS             complement(4798..5334)
                     /locus_tag="VLB45_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010190592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HSD04631.1"
                     /translation="MTRKKRLLFLLIFAAIFSVSYSIGAEMPVSTEDAEIFLKEFQLA
                     IEGIDAIGIFVHNISIALPMFIPGFGLIWGAFAAWSTGFAFSALETTNPILSQLPPLS
                     VLFLSPFGLMELCAYSIGMSRSFLLISTIIKKNPIKNELRPTIIEIGIVIGLLVVGGF
                     VEFEMIQQFGSDIDLPKS"
     gene            5412..6839
                     /locus_tag="VLB45_02645"
     CDS             5412..6839
                     /locus_tag="VLB45_02645"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed DNA polymerase II small subunit"
                     /protein_id="HSD04632.1"
                     /translation="MDENASSALNYALSKGFQIHPDAFKILEKIDSKELESVIRRVVR
                     EKAKQNLFLINQNDLRMFVDTEIDETLANDHVILSDPTKKITSAEGIDGFGALFVNRY
                     SKLLKIIMQRSQAKKLTQIETVKAGKPKEDVFVSGLLMDRKIERDVTKLVVDDPTGSI
                     ETLVFNKDLQLVANSLLIDQFVMQSVTTGKNGGFIAKELVVPDVPDHVTNRSKTETYA
                     VLISDLHVGSKYFMEKEFTEFVSWLSSPDPVAKKIRFVLVCGDVIDGVGIFPNQDKEL
                     LLVDIGQQMAKAAELLDKIPKHIKVFIIPGNHDPGRRALPQPAIPEKYNLNLWNRENF
                     FMVGNPALIELNGVKVLMFHGQSLDDVVGTTPGLSYMQPAKAMRVLLKSRHLSPIYGK
                     RTPIAPETEDMMVIDEVPDIFHSGHIHVVDLDMYKGTLIVNSGAWQSQTPYQTSVGIT
                     PTPGVAVIVNLATLKVFTKSFVDEL"
     gene            complement(6834..8045)
                     /locus_tag="VLB45_02650"
     CDS             complement(6834..8045)
                     /locus_tag="VLB45_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016939437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HSD04633.1"
                     /translation="MTNRDPVDRLLDDVASGRSLLKNRDVLHFSFIPDVILHRENELA
                     KVTQSLLPILKKSRPSNLLVYGKPGTGKTLVVKTVLSKIQKRVEKSDFPIKLVYANAK
                     DETTLYGLLVSFGRQLGLKTQKELPSTGLSISEVFNRLLEVIQKNTTNAVFVIDEIDY
                     LAELVSKTGKDILYNLTRANERLKTGSLTLIGISNDLTFKERLDPRVLSSLSEEEIVF
                     TNYTVNQITEILLGRIKEAFISGTVEQAAINLCAAMAGQEHGDARRAIDLLRVAGELA
                     EREQSDVVKEEHIRRASLKMEEDKETTALNSYPLHEKLLIIAIMRANGSSTGEIYQAY
                     KSLCKTIRQKEITQRRVTQILGDIELSGLITGKITHQGMHGRTKKYNLTIQSDVVKNA
                     FKNDLMLTDIL"
     gene            8520..8921
                     /locus_tag="VLB45_02655"
     CDS             8520..8921
                     /locus_tag="VLB45_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012216181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04634.1"
                     /translation="MTRKKIRIDLEDAEGAKYNFSLEGNVTKDKMLKIFELMDLMNIE
                     NQDEAPVLDSVGAKIWNIVDKHFPLGKFTSSKILEQYEDDYGEPIKLSIISTYLARFA
                     SKGRVARAREGREWTYQIAKIPQKQHNAFTN"
     gene            complement(8923..9084)
                     /locus_tag="VLB45_02660"
     CDS             complement(8923..9084)
                     /locus_tag="VLB45_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HSD04635.1"
                     /translation="MTVQQNEELVKITSGGTISIPKQFRKFLELQKGDYVKVIMSQDH
                     LIIKKIIIS"
     gene            complement(9187..9738)
                     /locus_tag="VLB45_02665"
     CDS             complement(9187..9738)
                     /locus_tag="VLB45_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014966077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding protein"
                     /protein_id="HSD04636.1"
                     /translation="MLVEIPDVGVMIGIFLAFIGGLFAIVIYNKIKPLIRTESDQIKS
                     YVERLQYYENQLIDMKIRMDSMELGKEEESFETAPKPERRQVRPGLDEKEQQDETSKE
                     RMRNMNFGNATDYVLKLITEKPMTSRDIQITIGRTREHTSRMMKRLFEEGYVERNMKT
                     KPFTYYITDKGKTKIGLVKVAQQ"
     gene            complement(9867..10229)
                     /locus_tag="VLB45_02670"
     CDS             complement(9867..10229)
                     /locus_tag="VLB45_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04637.1"
                     /translation="MAESPKPIFNKIIRQIIKKSLFTERQIEIILNQANLQKSQFTIT
                     KGAYYRQVKQSREKLIGLYYSIVLLKGLGVLLPEDIDVINRLSEQISVIQNSDVFPER
                     EDEIISVIEQLLHRACRV"
     gene            10294..11043
                     /locus_tag="VLB45_02675"
     CDS             10294..11043
                     /locus_tag="VLB45_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007551677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047294 - phosphoglycerol
                     geranylgeranyltransferase activity [Evidence IEA];
                     GO_process: GO:0006650 - glycerophospholipid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranylglyceryl/heptaprenylglyceryl
                     phosphate synthase"
                     /protein_id="HSD04638.1"
                     /translation="MTSVESYLHATLKKKKALLFVLIDSEVSAIKSATKLAKNVEKLG
                     ASAILVGGSSATDQLGMAQVVKTLKKSVKIPIILFPGNVTGVVPDADAILFSSLLNSE
                     NPYFISQAQALGAPSVLKFGLEALPTAYIVIGEGTSAWFVGSVRGIPFDKANIAAAYA
                     LSAQYLGMRFVYLEAGSGASSHVKPEMVRAVRKLFKGFLIVGGGIRNSQTANELVKAG
                     ANAIVIGTLAEKGGGLKVLADIVKTIQSARK"
     gene            11045..>14280
                     /locus_tag="VLB45_02680"
     CDS             11045..>14280
                     /locus_tag="VLB45_02680"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012216176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II large subunit"
                     /protein_id="HSD04639.1"
                     /translation="MSENVALRLGEIPMPKYYSDYYTALSNEVFSTFERAAAAKSTLA
                     DSSVMVEPKIAFDLADRVSKMHEIDVTERLRALIQTTTKELAALTLSEEIALGKYSST
                     DASLEEKLDMAVRVGLAIVTEGVTIAPLQGIHEVKIKKNRDGSEYLSISFAGPIRSAG
                     GTEAASTMLIADHVRKIAGLDKYQANSFDDETGRFVEELRLYEREAGNFQYHVPDEDV
                     VTVISNLPVELNGVDTDPFEIVSHRNMARIDTNRVRGGALRVLNDGLIGKSRKLLKLL
                     DLFKLNDWDWLKELKGAIQTGDEDAASHRMREVITGRSVLSMPKKLGGLRLRYGRACN
                     TGFASIGIHPVVAEILDHAIAVGTQIKLDIPGKASTIAFVDSLDTPIVRLKNGNVVKI
                     KDTAHGLSLKNKIEKILHLGDILISYGDFLENNAELVPTGYVEEYWVAELEAKLAKYE
                     SKDSALEYLSKIPSFDEALKLSLDFDIPLHPHYLYFWEQLSVEEFEQVLKPTRAEPDL
                     VIYQKSSKLILEKLGVPHEVSGDTILLKTPESRIFFYLLFRKPIELDNSKTVPEMISQ
                     TSGIKIRDKFSTAIGVRVGRPEKAAARKMKPPVHTLFPVGSKGGASRDLIKAAKQPNF
                     YCNISNRICKNCNELSISISCIKCKSKTTIIFICPQCRAEIDSDFCEKCNKKTSSHGY
                     KGFPLKERLYAAQEKTGIRVQEPFKGVRELINQDRIAEPLEKGVIRQSLGLSVFKDGT
                     IRYDATNAPLTHFKPTWIGTSLEKLAELGYTHDIDGKPITSPDQIIELRIQDVIIPYE
                     CGEYLVQTSQYIDKEMEKLYGHSVFYKVKTNNDLIGHLIIGLAPHTSVGIVGRIIGYT
                     NTHVCYGTPVWHSAKRRDADGDADSIMLLMDSLLNFSRQFLSDRIGGLMDAPLLIQPI
                     VIPQEVQRQAHNFEVEKILPLSFFEATVAKAKASEVKGIETLKSRLETEKQFYGHYFT
                     HMTSTLTTSRSRSAYSTLGSVLEKLDMQIRTADIINAVDPSEVVSMVMTTHLLPDIMG
                     NLRAYSGQSFRCTACGASYRRIPLSQNCTECGNNLIQTLTRPSVKKYL"
BASE COUNT         4424 a         2619 c         2790 g         4447 t
ORIGIN      
        1 acaagaactt tgatctggac tgctggtgta actccggttg atacgataaa gaaatccatt
       61 tttaaaacta gcagaggtgg agtgattgtt aatgaatttt tagaagttcc agaattccct
      121 ggtgtttttg cagtaggaga ttgttgttat gcattaaacc cgcaaaccca aaaaccttat
      181 ccacccactg cacaaaatgc tgaagcacaa gctaaaattg cggcacacaa tctacatgct
      241 ttaataaaaa aagatcaaaa aaagaaattt gaatactcat caaaaggaca gatggcagta
      301 ataggaaaaa aatctggaat agctacaatt tttggaataa atttacatgg gtttttggca
      361 tggtgggttt ggagaactat ctatctttca aaaataccaa gacttgataa gagaattaga
      421 attttacttg attggacagt agatttgttt tttgatagag atattgcaag attgaaaata
      481 attagacgcg aggcacccaa ggactacaag gagcttgatg aagtagatga cgtatggtaa
      541 atgattctat ggcaaattat ttttttgtaa tggtttcttt tttcttttga taacaaatat
      601 tgctgcaacg gcggcaacag caatgcctat ggctatacca tattcgtatg gaaagtttac
      661 gggtttgttt ttttgtgtag aggtaggttc aaaagaatcc attatgtcgt taaaagtgcc
      721 tatataggaa tcaaaattct tttcttggtt agtataagtt aacgtataga atttgtcatt
      781 aatttcaaag acaatttctt ggtattttat tatgaatatc ttatcttttg ccacaaaccc
      841 acctcgtgcc tcagtgatgt gagcatcata atttttgatc tttttatttt cttcagaaat
      901 gatttgtagt ctgccagcat caataagtgg ttgtagatct ttttttgttt gtgtaatatg
      961 tgtatcaagt gaggttccgt tagaattctc aacgagaaaa gagactacgg gtgtaaactc
     1021 gtctattact ggaccaacta caactacatc gggagcttct ggttgtgttt ttgctggttc
     1081 ttgtacaaac catccttctg gtgccttgaa agaaaaacca aacagtgtgt tgtaataatt
     1141 tccttcctcg tctaaggtgt aatgtgtaac aatacgttct ggttcaccaa gatttaatat
     1201 gtttgaaatc tcggacatgt tcttgatctt catactgtaa atttctgcat cagcatattg
     1261 aagtggaatg ttaactggaa ttccacttgc aatatcatgt acagtttcaa gtgaagcaat
     1321 tttgtcaagc acgtcaaagc tttcttgtgt tactagtcta ccaaatgcag tgtatttacc
     1381 atccaaatgc ggtgaatcct tgtgtacaat aaaaaattgt gagcttgcac tatcaggatc
     1441 agctgcacgt gccatagata tgattccccg tttgtgttga atgttgttaa attctgcctt
     1501 gatcgtatac cctgcctcac cggttcccca ttgttgtatg agacgactgt ccttggacag
     1561 tggatcgcct ccctgaatca taaaaccggg tatgattcta tgaaattttg taccatcata
     1621 gaaaccactt tctgccagtt tgataaaatt atcaacggtg tttggtgcat cgtttggaaa
     1681 aaactctatt accatattgc caaattttgt ttctatcaca gctactttag aatcagaaaa
     1741 gcttgttgga accaagttta ctaaaagtaa aaaagataca ccaaatgcaa atattttaaa
     1801 attcaatatt gttggtattt caaattcgtt aataaaagcc aactgttaca cattggtttt
     1861 taatatgcaa atttgaacag agtttatgga ttcagatgaa cacatacaaa cccacctagt
     1921 ttccgtgtgg ggagaagaaa agaaattcct aaagcgaggt gcggaaggaa tgctactttt
     1981 aacagaaaag catgtgatgt ttgtaacaaa aactgaagca aaaatgaagt ggtggacaac
     2041 tgcaactgat agacaactta gaactctgtt aaaatcaaac aacataatga ttcatcagga
     2101 tggttacaat gaagaggatt tgcgacgtga tcttgaaaat gacgcaaatt tggaattttc
     2161 ttttgataga attttgaatg tggattttga agaaaagcca tgggcgccta ttttaaagtt
     2221 ggaaatcaat atgggagaca agaccaaaaa ataccacttt gcaattgtgg gtgggtgggt
     2281 aaaatatcca ttaaaagatc ctgtaaagta tgtcaaggtt gattgggctc cctttgttga
     2341 atatattaaa agtagacaaa aaatcacgga gtaagaaatg ggcgaagttt atgcggtagg
     2401 tgtaggtcct ggttcaccaa gatatgttac tgatgcggta aaggaagtca tattgtattc
     2461 tgatgtggtg ataggttaca aatacaccct taaaacaata gagagactcc tttcaaacaa
     2521 acaagtgttt gaaattacaa tgcaaaatca agaagaagtt taccaaatag ttgcaaagac
     2581 attaggtgac aaaacctgtg tgattccatt tacaggtgac gtgaattttt cagaatcaga
     2641 agttgttgat agattaattg agatatttgg agatgtgcag atcattccag gaattagttc
     2701 aattcaggtt gctgctgcaa aagcgcgtat tcctttagac aagagcaagg tgattacaat
     2761 gcatgtgaca acaagtattg aggaaaaaaa acttgaactg caaaaagcat tactggatgg
     2821 atatagtgta atattggtac caagaccctg gccaaaagat ccatcaaaac attttatgca
     2881 gtctgaaatt gctttttatt taaaaaacca tgggtttgac acctcaaaga taaaagttca
     2941 tgtttttgag ttcttgacta cagataagga gacgtcgttt gtagggatgc ttaaggaatt
     3001 ggaaggcagg cagttttctg atctatctgt aatggtgttt gatcaaacaa aacttgattc
     3061 ctatatcaat tttaaaacaa attagattct caaacttgta cttgttttta atatccaatg
     3121 aatcttattg tgatttagtg gaaaacgatt ttgtttcagg aagtgaaatt attactccat
     3181 ccatccagta atatgttgtg tcaaagaatc ttgtgatacc caagttgtct attgtgacac
     3241 tgaatgtttg gccgggggca atatcaccac tatcaatttt accatctggt ttgaatggtt
     3301 ttccaggaga aaagcttgca accccactca tgattctatg tgacaccgag tcttcgtttt
     3361 tccatatgat ggttgtaccg ggtgcaatct caacagtatc tatggaataa tagttgatgt
     3421 ttccggggtt tgacgcacca gccagaatgt ttattcgaat agtttttgtt tctttttctg
     3481 gttcttctgt tggtagtaca acattagtac caactatttt tacattgtaa actaccttca
     3541 ttacctcatc atcttttgtt agtctcccag taagtgtgta gatagaacct tcttcgctgt
     3601 tttccaccag ccttccagtc aatcttagtg aaatttcatc accgtctaaa ttctcagcat
     3661 tgccagcaag tgtaagaaat ctaccctcac gcagtattgt acctgtccat ccatcagaaa
     3721 ggagatactc atcgtttgaa attgatgtta ctccatcttc gattgtgatt ttgatcctgc
     3781 tactggtttt gcttcctgtt gaaaactgaa aatccaattc agaatcttcg attatttttt
     3841 ctgtaataat aaaaccattt ccttgtaaga ggtatatgcc gttgggaagt agctcagaat
     3901 ttgcttgttg tactgaacta gcaagtacaa ttccagtaat tgagattagt agtaaggcaa
     3961 tgagtttcat tgattaatta agtctcaaag ttctatataa ttaatggtct aaattattta
     4021 gactggttag gcaattcctg gctgtttgtc tcttctcata agaccatcca tgtaattcat
     4081 ttgttgctcc atgaattttt tctttttttc ttcctcttct aactctttca tgtcaaaagg
     4141 tggaattttt agaaattcca aaagtgagga gagaatcatt tgcgcagttt ttggctggat
     4201 tacgtttggg ttgtctattt ctccaagaat gcaaacgctg tctatgtttt tttcttgagc
     4261 aatgccgaga atcaggccat taaaaccagt tatctgacct ttaccatcta gaagattcat
     4321 tttttgtttt tttagttggt taagaagtgt aggtgatgtt gttgcgccga aaatttttgg
     4381 tgctgtagga tcaggttgtc ttaatgctgc accaatagag taaagggttg taactttgag
     4441 tttcttggcc atatctatca cctcataaca tatttcgtat aatcgccgtg gatcagaagg
     4501 attgaattct cctgaaaaga ttatcagatt ttcttttttt gaataataaa atttgtaact
     4561 tgactgtgtg tattttatca ggccggcatc gtatgatact gccggtggcc aatacgcata
     4621 aagctcggca aataactttg cctttaattt ttttacaaga aaatccattc caataccagc
     4681 tacattaccc atgtcaggca gcgcagcaac catgacagga tttgttagtt ttggaagctc
     4741 gtgaattttt agccccagca ttaatttttg atattttatg tcaagataaa aacgaatcta
     4801 agattttggc aaatcaatat ctgaaccaaa ttgttgtatc atttcaaact ctacaaatcc
     4861 tccaacaact aacagtccta tgactatgcc aatttctata attgtggggc gcagttcatt
     4921 tttgatagga ttttttttga ttatggtgct tatgagaaga aaacttctag acatgcctat
     4981 ggagtatgca caaagttcca tcaacccaaa aggtgaaaga aagagaacgc tgagaggagg
     5041 tagttgtgag agaattggat ttgttgtttc tagtgcacta aatgcaaatc ctgtagacca
     5101 agctgcaaac gccccccaaa tcaatccaaa ccctgggata aacatgggca atgcaatgga
     5161 aatgttgtgt acaaatattc caattgcatc aattccttct atggccaatt gaaattcttt
     5221 aagaaagatt tctgcgtcct cagtggatac tggcatttca gcgccaattg aatatgatac
     5281 cgaaaatatg gcagcaaaaa ttaggagaaa caaaagtctt ttttttctgg tcatgatttt
     5341 attttcttgg atctctattt gagggttggg agctgagcag atttaattaa aggttgtaat
     5401 atttgtggtt gatggatgag aacgcttctt ctgcactaaa ttatgcgtta agtaagggat
     5461 ttcaaattca tcctgatgcg tttaaaattc tagaaaaaat tgattcaaaa gaactggaat
     5521 cagtaatcag acgagttgtt cgtgagaagg caaaacaaaa cttgttttta ataaatcaaa
     5581 atgatttgag gatgtttgtt gatactgaaa ttgatgaaac acttgctaat gatcatgtaa
     5641 ttttatcaga cccgacgaaa aaaatcacat cggctgaagg aattgatggt tttggagcct
     5701 tgtttgtaaa ccgatactca aaactcctaa aaataatcat gcaaagatcc caagcaaaga
     5761 aactgaccca gattgagacg gtaaaggcag gcaagccaaa agaggatgtg tttgtctctg
     5821 ggcttctcat ggaccgtaaa attgagagag atgtcacaaa gcttgtagtt gatgatccta
     5881 ctggctccat tgagacgctt gtttttaaca aggatttgca attagttgca aactcgcttt
     5941 taatagatca gtttgtgatg cagtctgtaa ctactggaaa aaatggtggt tttatcgcaa
     6001 aagagcttgt ggttccagat gttccagacc atgtaacaaa ccgctccaaa acagagacct
     6061 acgcagtatt gatttcagat ttgcatgttg gcagcaaata ctttatggaa aaagagttta
     6121 cagaatttgt ctcttggctt tctagccctg atcctgtagc aaaaaagatt cgatttgttc
     6181 tagtttgtgg cgatgttata gatggtgttg gaatctttcc aaaccaagac aaagaattgc
     6241 ttttagtgga tattggccag caaatggcaa aagctgcaga gttacttgat aagattccaa
     6301 aacacatcaa agtgttcata attccaggaa accatgaccc tggtcgtaga gcactaccac
     6361 aaccagcaat accagaaaaa tataatttga atttgtggaa tagagaaaat ttcttcatgg
     6421 ttggtaatcc cgccttgatt gagctaaatg gggttaaggt tttgatgttc catgggcaaa
     6481 gccttgatga tgttgttgga actacccctg gtcttagcta tatgcagcct gcaaaagcaa
     6541 tgagagtttt gctaaaatca aggcacctaa gtccgatata tggaaaacga acacccattg
     6601 cacctgagac tgaggacatg atggtcattg atgaggtccc tgacattttt cattctggac
     6661 atattcatgt ggttgacttg gacatgtaca aaggaacttt gatagtaaac tctggagctt
     6721 ggcagtctca gactccatac caaacaagtg ttggaattac accaactcct ggggtagcag
     6781 taatagtaaa cttggcaaca ctcaaggttt tcacaaaatc ttttgtggat gaattataga
     6841 atatctgtta gcattagatc atttttgaat gcgtttttta ccacgtcaga ttgaattgta
     6901 agattgtatt tttttgtcct accatgcatt ccttggtggg taattttacc tgtaatcaag
     6961 ccggaaagtt caatgtctcc tagaatttga gtgactcttc tttgggttat ctctttttgt
     7021 ctgattgttt tacatagact tttgtaagct tggtaaatct cccctgtaga tgagccgttt
     7081 gctctcatta ttgcaattat gagcagcttt tcatgaagtg ggtaggagtt tagtgcggtg
     7141 gtttccttat cctcttccat tttaagggaa gcccgtctga tgtgttcttc ttttaccaca
     7201 tcagattgtt ctctttcagc caattctcct gcaacacgta gaagatcaat tgcgcgcctt
     7261 gcatctccat gttcttgacc agccatggca gcacagaggt ttattgcagc ttgctctacc
     7321 gtcccagaaa tgaatgcctc tttgattcgc cctaagagaa tttctgtaat ttggttaaca
     7381 gtataatttg taaagactat ttcttcctca cttagactgc ttaacactcg tggatctagt
     7441 ctttctttga atgtaagatc gtttgagatc cctatgagag taagtgatcc tgtttttagt
     7501 ctctcatttg ctcttgttaa attatacaag atgtcctttc ctgtttttga gactagttct
     7561 gcgagatagt caatctcatc aataacaaaa actgcgtttg ttgtattttt ttgaattact
     7621 tctagtaacc tattgaacac ctcactgatt gacaggccgg ttgacggtag ctctttttgt
     7681 gttttcaatc ctagctgtct tccaaaactt actagtaatc catacaatgt ggtttcgtcc
     7741 ttggcgtttg catacactaa ttttattggg aaatcgcttt tttcaacacg tttttggatc
     7801 ttggataaaa ctgtctttac cactagtgtt ttaccagtac ctggttttcc gtaaacaagt
     7861 aggttggacg gtcttgattt ttttaaaatt ggtaacaatg actgtgtgac tttggccagc
     7921 tcattttccc tatgtaagat tacgtcgggg ataaacgaga aatgcaatac atcacgattt
     7981 tttaaaagtg accgtccact tgccacatcg tcaagtaatc tgtctactgg atcacggttt
     8041 gtcaagtacc cacacaccta catttcttct ctgtccacat atggactcta cttaagataa
     8101 cccaattaga atagaatcac taaaccaaat ttagtttata attaattatt tttattttct
     8161 cttaaaaaaa attttttaat taagctagaa tggaaacatt ggtgtgtggg atgcgtgcaa
     8221 tctggttaac tatttgttaa ttgtttgtta aactagaacc agaaaaggct agttttgacc
     8281 cctttatttc ctgtccaggc gtgaaataag gtcttaacaa tgttaatttg gcaggcctga
     8341 cccctatgtt tccactccag gcatgagttt cctgcattgg gtatggacta ggaagactag
     8401 cctgttaagg tacaaaaaat ggctaggcgt gggttgggca gtgaaagtta acaaactcac
     8461 tagttaacaa atcttctttg atctgttcaa gtgttaaggt gttaacccaa tcgatcacaa
     8521 tgacaagaaa gaagattcgt atagacctag aagatgcaga gggtgcaaag tacaatttta
     8581 gcctggaggg taatgtcaca aaggacaaaa tgctcaaaat attcgaatta atggatttga
     8641 tgaatattga aaaccaagat gaagcaccag tactagactc ggtgggtgcc aaaatctgga
     8701 acatagtgga caaacacttt ccacttggca agtttacctc atctaaaata cttgaacaat
     8761 atgaggatga ttatggggaa ccaatcaaac taagtataat ttctacatac ttggcaaggt
     8821 ttgcctcaaa gggtagggta gcaagagcta gagaaggtag ggaatggacg tatcaaatag
     8881 ccaaaatacc tcaaaaacaa cataatgcat tcactaacta actcaagata ttattatttt
     8941 tttgatgatg agatgatcct gactcatgat tactttgaca taatctccct tttgcaactc
     9001 aagaaacttt ctaaattgtt tgggaattga gattgtgcct ccagacgtaa tcttgacaag
     9061 ctcttcattc tgttgtacgg tcatataata aataattagt ttaatgatta tatattttac
     9121 tgttttaata aaatattatt ttggtcattt tgggtggtaa aaccaactaa aattcctata
     9181 attctattat tgttgtgcaa ccttaaccag accgatctta gttttaccct tatccgttat
     9241 gtaataggta aagggtttgg ttttcatgtt cctttcaaca tatccttctt caaacaacct
     9301 cttcatcatc ctggatgtgt gctctcttgt cctacctatt gtgatttgta tgtcacgtga
     9361 tgtcatcggt ttctctgtga ttagcttcaa cacataatct gtagcattac caaaattcat
     9421 gttacgcatg cgctcctttg aagtctcatc ctgctgttcc ttctcatcta atcctggcct
     9481 tacctgtctt ctttctggct ttggtgctgt ctcaaaactc tcttcttcct tacccaactc
     9541 catagaatcc atgcgtattt tcatgtctat tagctggttc tcatagtact gtagacgctc
     9601 tacatacgac tttatctgat cagactcggt ccttatgagg ggttttatct tattgtatat
     9661 cactatggca aaaagaccac ctatgaacgc aagaaagatc ccaatcatca cacctacatc
     9721 gggaatctct actaacatat cacgaaaatt cagataataa tgtgatttat catatttggg
     9781 taactatttt tcacataata cacactaatc acaacatgac cactatcatc aataattata
     9841 atttgtcaca accaaataat acaaaatcac actctacagg cacggtgtag tagttgctct
     9901 atcacagata tgatttcatc ctccctttca ggaaaaacat cactattttg tatcacagaa
     9961 atctgctcag ataatctatt aatcacatca atatcttcag gaagaagcac gcctagaccc
    10021 tttagtaaca ctatggagta atacaaccca attagcttct ccctagactg cttaacttgc
    10081 ctgtaatagg cgccttttgt tatagtaaac tggcttttct gcaggtttgc ctgatttaga
    10141 ataatttcaa tctgtcgttc agtaaacagt gattttttta taatttgcct tataatttta
    10201 ttaaaaattg gctttgggga ttctgccata gctatacaat tctgtataca tgattcgtat
    10261 taaactttaa aaagttcatg attaaccaga cttatgacaa gcgtagagag ttatcttcat
    10321 gctactctca agaaaaagaa agcgctgctt tttgttctca tagattctga ggtatctgca
    10381 ataaaatctg cgacaaaact tgcaaaaaac gttgagaagc ttggagcatc tgccatactg
    10441 gttggaggat catcagctac ggatcagtta ggcatggcac aagtagtcaa aaccttgaaa
    10501 aaatctgtaa agattccgat catacttttt cctggtaatg ttacaggagt tgttcctgac
    10561 gccgacgcaa ttctatttag ctctcttttg aactcggaaa acccatactt tatctctcaa
    10621 gcccaagccc taggggctcc aagtgtactc aagtttggtc ttgaagctct acccactgct
    10681 tacatagtca taggcgaggg gacatcagct tggtttgtag gttccgtgag aggaatcccc
    10741 tttgataaag caaacattgc tgcagcatat gccctctcag cacaatatct tggtatgaga
    10801 tttgtctatc ttgaagctgg ctctggtgca tcatcccatg taaaacctga gatggtaagg
    10861 gcggtacgaa aactattcaa aggtttcttg attgtaggtg gcggaatacg aaactctcag
    10921 actgcaaatg aacttgtaaa ggcaggagct aatgcgatag tcattggcac ccttgcagaa
    10981 aaaggcggtg gtctcaaggt tctagccgat attgtaaaaa ctatccagag cgcaaggaag
    11041 taacatgtct gagaatgtgg cattacgact aggcgagatt ccaatgccaa aatattattc
    11101 tgattattac accgctctct caaatgaggt attttccacc tttgagaggg cagctgcagc
    11161 aaaatccaca cttgctgact cgtccgtaat ggtggaacca aaaattgcat ttgatctggc
    11221 agaccgtgtc tcaaaaatgc atgaaatcga tgtgacagaa cgtctaagag cactaatcca
    11281 aaccacaaca aaagaacttg ctgccctgac actttcagaa gaaatcgccc ttggcaagta
    11341 ttctagtact gatgcatcac ttgaggaaaa gcttgacatg gctgtaagag taggccttgc
    11401 cattgtaaca gaaggcgtta ccatagcccc cctgcaagga attcatgagg tgaaaataaa
    11461 aaagaaccgc gatggctctg aatatctttc catatccttt gctggaccaa tcaggtcagc
    11521 aggtggaaca gaggcagcat ccaccatgtt aatcgcagat catgttagaa aaattgcagg
    11581 ccttgacaaa taccaggcaa attcctttga tgatgagact ggcagatttg ttgaggaatt
    11641 gcggctctat gagagggaag ctgggaactt tcaatatcat gttccagacg aggatgttgt
    11701 cacagtaata tccaatcttc cagttgagct aaacggagta gatacagatc catttgaaat
    11761 tgttagtcat agaaacatgg ccagaataga tacaaacaga gtaaggggcg gggcactacg
    11821 tgttctaaat gatggactga tcggtaaatc tagaaaactg ctaaaactac ttgatttgtt
    11881 taaactcaat gattgggatt ggttaaagga actaaaagga gctatccaaa caggcgatga
    11941 ggatgctgcc tctcatagga tgagagaggt cataactggc aggtctgtcc tctcaatgcc
    12001 aaaaaaactg ggtggattaa ggctgcgata tggaagggcc tgcaacacag gctttgcctc
    12061 aattggaata catccagttg ttgcagaaat attagatcat gccatagctg ttggcacaca
    12121 aatcaaactg gacattccag gtaaggcatc aaccattgca tttgttgatt cccttgatac
    12181 tcccattgtt agactaaaaa atggaaatgt tgtaaaaatc aaagatactg cacacggtct
    12241 aagtctaaaa aataaaattg aaaaaattct tcaccttggt gacattctga ttagctatgg
    12301 tgacttttta gaaaacaacg cagagcttgt acctacagga tatgttgagg aatactgggt
    12361 ggctgagcta gaggccaaac ttgcaaaata cgaatcaaaa gactcggctc tagaatacct
    12421 ttcaaaaatt cctagttttg atgaggcact caaactatcc cttgattttg atatccccct
    12481 tcatcctcat tacttgtatt tttgggaaca actctcagtt gaggaatttg aacaggttct
    12541 aaaaccaact agggcagaac cagacttggt catctaccaa aaaagtagta aactgatttt
    12601 ggagaaactt ggggttcccc atgaagtttc tggcgacaca attttactaa aaactccaga
    12661 atctaggatt ttcttttatc tgctttttag aaaaccaatc gaattagata attccaaaac
    12721 agtacctgaa atgatatccc aaacttctgg aattaagatc cgtgacaaat tttctacggc
    12781 aattggagtt agagtaggaa ggccagaaaa ggcggcggca agaaagatga agccgccagt
    12841 tcatacactg tttcctgttg gcagtaaggg tggtgcctca cgtgacctga tcaaggcagc
    12901 aaagcaacca aatttctatt gtaacataag taacaggatc tgcaaaaact gtaacgagct
    12961 atcaatcagt atatcctgca taaaatgtaa aagcaaaacc acaataattt ttatctgtcc
    13021 acaatgcagg gctgagattg attctgattt ttgtgaaaaa tgtaacaaaa agacgtcatc
    13081 acatggttac aagggatttc ctctcaagga gagactttat gctgcccaag aaaagaccgg
    13141 cattagggta caagagccat tcaaaggcgt tagagaactc ataaaccaag acagaatagc
    13201 agaaccactt gagaaaggag tcattaggca aagtctgggc ctaagtgtat tcaaagacgg
    13261 aaccatacga tatgatgcaa caaacgcacc actcactcat ttcaaaccaa catggattgg
    13321 tacatcacta gagaagctag cagaacttgg ttatacacat gacattgatg gcaaaccaat
    13381 tacaagtcct gaccaaatca tagaactaag aattcaagat gtcatcattc cttacgagtg
    13441 tggagagtac cttgtccaga catcccaata catagacaag gaaatggaaa agctctatgg
    13501 ccattctgta ttttacaagg tgaaaaccaa caacgacctt attggtcatc tgataattgg
    13561 tctggctcct cacacatccg ttgggattgt tgggagaata attggatata caaatacaca
    13621 tgtgtgctat ggaacgcctg tgtggcactc tgcaaagaga cgtgatgctg acggtgatgc
    13681 tgactcgata atgcttttga tggacagcct gcttaacttt tcaagacaat ttctatcaga
    13741 cagaattggt ggtcttatgg atgcaccatt attgatccag cctatagtta taccgcaaga
    13801 agtgcaaaga caagcacata actttgaagt agaaaaaata ttaccacttt cattttttga
    13861 ggccacggtt gcaaaagcaa aggcaagtga agtaaaagga attgaaactc tcaaatcaag
    13921 acttgagaca gaaaagcagt tttatggaca ttactttaca cacatgactt caactctcac
    13981 aacatctaga tctagaagcg cctattccac acttggttca gttttggaaa aacttgacat
    14041 gcaaattagg accgctgata tcatcaatgc agtagaccca agtgaagtgg tatcaatggt
    14101 aatgacgaca catcttttac ctgatataat gggcaaccta agggcatatt ctggccagtc
    14161 gtttagatgc actgcttgtg gtgcaagcta cagaagaatt cctctttcac aaaattgtac
    14221 agaatgcggt aataatctga ttcaaacact aactagacca tctgtcaaaa aatatctcaa
//
LOCUS       DATIDL010000061         4015 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrosopumilaceae archaeon isolate SMAG_U16342
            k141_2493758, whole genome shotgun sequence.
ACCESSION   DATIDL010000061 DATIDL010000000
VERSION     DATIDL010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935836
            Sequence Read Archive: SRR25158326
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosopumilaceae archaeon (soil metagenome)
  ORGANISM  Nitrosopumilaceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrosopumilales;
            Nitrosopumilaceae.
REFERENCE   1  (bases 1 to 4015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/04/2024 05:09:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,520
            CDSs (total)                 :: 1,474
            Genes (coding)               :: 1,474
            CDSs (with protein)          :: 1,474
            Genes (RNA)                  :: 46
            rRNAs                        :: 1 (5S)
            complete rRNAs               :: 1 (5S)
            tRNAs                        :: 43
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4015
                     /organism="Nitrosopumilaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2493758"
                     /isolate="SMAG_U16342"
                     /isolation_source="soil"
                     /db_xref="taxon:2202732"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            65..1396
                     /gene="cca"
                     /locus_tag="VLB45_02685"
     CDS             65..1396
                     /gene="cca"
                     /locus_tag="VLB45_02685"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008297095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HSD04640.1"
                     /translation="MKILDQAKKLAVPTEKEQEKIKKIAEISLQLVKEQAAKFPEVTS
                     VEIGGSYAKGTWLSGEVDLDIFVKLKKETDEKTFEAVGKKIGFDSLKKYKPYVRYSEH
                     PYVEAEIDKTRVNVVPCYDVELGQWKSAADRSSFHTKHMLQSLDQSKKDEVRLLKKFL
                     ISTGIYGAEIAKEGFGGYVAEVLVLHYGSFLGVLEAASNFVQNQVIGNPTKKFDTSLI
                     LIDPIDSNRNLGTAISAESAGKFVLASRTFLKKPTISFFKTRPSQTNRKNLDDVIVVK
                     FNYKKRSPDIIWGQIKRAATAVAGQLNLAGFEVLRKSAITDEKSEAALLFLLHSAKIE
                     KTVIKNGPEVFRRKESENYIAINSKKSQLMWVNENGKILSMQKRSYHDAKLFLQFLLK
                     KNLLASGVPSGLVSDIRRGFKTLNGKQVTSKSIKKALSELITTDGFVFGSP"
     gene            1371..2138
                     /locus_tag="VLB45_02690"
     CDS             1371..2138
                     /locus_tag="VLB45_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010194815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HSD04641.1"
                     /translation="MDLSLVPLSKLAREPYSTILGYPKATKRELSSRVSELKKLGVKG
                     IFFSGEATLNSLQVLGKGYVGVVVLSKYKTKDVALKIRRMDSSRPEMGSEARLLRLAN
                     EVNVGPKLIASSKNFMIMEYLEGKKIINWITELKGKGSAAKLRKVVRKVLEDCYNLDK
                     IHLDHGELSHIHKHVIVGKSISIIDFESSSTNRRTSNVTSATQSIFIGSGIAKLVKKI
                     IKEPKKKQLISSLRKYKNEQTRKNFESVLRVLGLQKT"
     gene            2135..3496
                     /locus_tag="VLB45_02695"
     CDS             2135..3496
                     /locus_tag="VLB45_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HSD04642.1"
                     /translation="MSLQNLKTQTLKLSLGLIISAFIVELSVGLYSNSLALITDSVHA
                     VLDSFVTAILLIAARLAAKPPDRQHTYGHGKIETMGGFIGGIVILLIAVFFIYEAIER
                     FQNPPEMLPGIFAVWAAVYTLGTDGVRITILGRALKKVEGSTTLRADLYHAFMDMGST
                     LVAIIGILLVAVGFNQGDIIAALVLGTLLVILSARLIHRTALELTDAIPESMVSKVRN
                     IVSNTEGVMNTESVQMRRSGHEIFADVTIALSGAASFDEAHKISSNVEKNITSSISNA
                     KVTVHFEPSWKDVPLDSQISNITSSVKGVEGVHNVSSSSSKEGIFISLHVMVDRNMSL
                     EEAHMVSDEIERQIRNVVNNINHITVHLEPHISLPKSIPVEDLTIEQQITKTIKEYPA
                     VKKIGRVITFQLQDIFKIDIDCSFDGTLSIEEVHDMVSEIEHKIRNQIKNAIITIHPE
                     PTQ"
     gene            3496..>4015
                     /locus_tag="VLB45_02700"
     CDS             3496..>4015
                     /locus_tag="VLB45_02700"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04643.1"
                     /translation="MKEQKAIRFLKQDPHLSEIIKQVGSYQIKQRNHHFASRVEAIIS
                     QQLAGAAAYAILQRFKKLYPTFPKPADVLDTKDSELRLVGLSKMKIEYLKDLAKKIEH
                     GEIKIKTLSKMTDEEVISHLTQVKGIGRWTAEMFLIFSLERQDVFPVGDLGLRKGVQK
                     AFSLKELPNPKDV"
BASE COUNT         1449 a          705 c          803 g         1058 t
ORIGIN      
        1 agaaaagtat cttaactcca agtggaccta tatactctga tttacaggtg gtaaaagcaa
       61 aacaatgaaa attttagacc aagcaaaaaa acttgcagta cccactgaaa aagaacaaga
      121 aaaaataaaa aaaatagcag aaatctcatt gcaactagta aaagaacagg cagcaaaatt
      181 tcctgaagtt acaagtgtag agatcggcgg atcttatgca aaagggactt ggctttctgg
      241 agaagtggat ctggacattt ttgtcaagct aaaaaaagag actgatgaaa aaacatttga
      301 agctgtagga aagaaaattg gttttgattc tctcaaaaaa tacaaaccat acgtcagata
      361 ctctgagcac ccctatgtcg aggcagaaat tgacaaaaca cgagtaaacg tagtaccttg
      421 ctatgatgtg gaacttgggc aatggaagag tgctgcagac agatcctcat ttcatacaaa
      481 acacatgcta cagtcacttg accaatcaaa aaaagatgaa gtaagactgc tcaaaaaatt
      541 tctgatcagt actggaatct atggtgcaga aatagcaaag gaaggatttg gtggttatgt
      601 tgcagaagtt cttgtcttac actatggaag cttccttggt gttttagaag cagcatcaaa
      661 ctttgtgcaa aaccaagtca taggaaatcc tacaaaaaaa ttcgacacct ctctcatact
      721 gattgatccc atagacagca acaggaacct tggaactgcg atttcagctg aaagtgctgg
      781 caagtttgtt cttgcttcca gaacatttct gaaaaaacca acaatatcat ttttcaagac
      841 aagaccaagt caaacaaaca ggaaaaactt ggatgatgtg attgttgtta aatttaacta
      901 taaaaaacga agccctgaca taatttgggg tcagataaag cgtgcagcaa ccgctgtagc
      961 aggtcagctt aatcttgcag gctttgaagt attgagaaag tctgccatca cagatgaaaa
     1021 gtcagaagca gcgctgcttt tccttttgca ttctgcaaaa attgaaaaaa ctgtgatcaa
     1081 aaacggaccc gaagttttta gaagaaaaga atctgaaaac tatattgcaa taaattctaa
     1141 aaagagtcag ctcatgtggg tcaatgaaaa tggcaagatc ttgtcaatgc aaaaaagatc
     1201 ataccatgat gccaagctat ttttgcagtt tcttttgaaa aaaaatctat tggcatcagg
     1261 tgttccaagt ggtctggtaa gtgacattag gcgcggattc aaaacgctaa acggaaaaca
     1321 agttacaagc aaatccatta aaaaggcact atcagagctt atcactacag atggatttgt
     1381 ctttggttcc ccttagcaag cttgcaagag aaccatattc tacaatactt ggatatccca
     1441 aggcaacaaa acgagagctt tcaagtcgag tgtccgaact caaaaaactt ggagtaaaag
     1501 ggatattctt tagcggtgag gccacgctta actctctaca ggtactaggc aagggctatg
     1561 ttggagtagt ggttttatca aaatacaaaa caaaggatgt agccttgaag attcggcgaa
     1621 tggattcaag cagaccagag atgggaagtg aagcaagact gttaagactt gcaaatgagg
     1681 ttaatgtcgg tccaaaactt atagctagta gcaagaattt catgattatg gaatatttgg
     1741 aaggaaagaa aataatcaat tggattacag agctaaaagg aaaaggaagt gcagcaaagc
     1801 tgagaaaagt agtaagaaaa gttttggagg attgttataa tttagacaag atacatcttg
     1861 atcatgggga gcttagccac atacacaagc atgtgattgt tggaaaatct atttccataa
     1921 tagattttga aagttcaagc actaacagaa gaacttctaa tgtaacatca gcaactcaaa
     1981 gcatctttat tggctctgga attgcaaaac ttgtaaaaaa aataattaaa gaacccaaga
     2041 agaaacaact catttcttct ctaagaaaat acaaaaatga acaaacaaga aagaactttg
     2101 aaagtgtgct tcgtgtattg ggattacaaa aaacatgagc ttacaaaatc ttaaaaccca
     2161 aaccctgaag ctttcccttg gtctaataat ttctgcattc atagtggagc ttagcgtagg
     2221 tctgtactct aacagccttg cattgattac agacagtgtt catgcggttc tagactcttt
     2281 tgttactgct attctgctta ttgcagccag attggcagca aaacccccag atcgtcaaca
     2341 tacctatggt cacggcaaga tagagacgat gggcggcttt attggaggca tagtaattct
     2401 cctcattgca gtttttttca tatacgaagc aattgagagg tttcagaatc ctcccgaaat
     2461 gttacctggc atatttgcag tctgggctgc agtgtataca cttggtacag acggagtaag
     2521 aattacaatt cttggcagag ccctgaaaaa agttgaggga tctactactc taagggcaga
     2581 tctttatcac gcgtttatgg atatgggttc tacactagtt gcaataattg gaattttgct
     2641 agttgcagtt ggatttaatc aaggagatat cattgctgca ctggttttgg gaacattgct
     2701 tgtaattttg tcagcaagac tcatccacag aactgcgctt gagcttacag atgcaattcc
     2761 tgaaagcatg gtaagcaaag ttcgcaacat tgtaagcaat acagaaggag tcatgaatac
     2821 ggaatctgtc cagatgcgca gatctggtca tgaaattttt gcagatgtta caatagcatt
     2881 aagtggtgct gcaagttttg atgaagctca caaaataagc agcaatgtag aaaaaaacat
     2941 tacaagttct atttcaaatg ccaaagtaac agttcatttt gaaccaagct ggaaagatgt
     3001 tcctcttgat tcacagataa gtaacatcac atcttctgtg aaaggagtgg aaggtgttca
     3061 caatgtcagc tcctcctcat caaaagaagg aattttcata agcttgcatg taatggtgga
     3121 taggaacatg tcgcttgaag aggctcacat ggtttcagat gagattgaga gacaaattcg
     3181 aaatgttgtc aacaacataa accatataac agtgcatttg gagcctcata tttcactacc
     3241 aaagagtatt cctgtagagg atctcacaat agagcaacaa attaccaaaa caatcaagga
     3301 atatccagca gtcaagaaaa ttgggagagt tattactttt cagcttcaag atattttcaa
     3361 gatcgatata gattgctcgt ttgatggaac tctttccatc gaggaagttc atgacatggt
     3421 atcagaaatc gaacataaaa taagaaacca aattaaaaat gctataataa caattcatcc
     3481 agaacctact caataatgaa agagcaaaaa gcaattcgtt ttctaaaaca ggatccacat
     3541 ctttctgaaa tcatcaaaca agttggcagt taccaaataa aacaacgaaa ccatcacttt
     3601 gcctcacgag tagaagccat tatttcgcaa caacttgcag gtgcagcagc ttatgccata
     3661 ctacaaagat tcaagaaatt atatcccaca tttccaaaac ctgctgacgt tttggataca
     3721 aaggattcag aattacgttt ggttgggtta tctaaaatga aaatagagta cctaaaagat
     3781 cttgcaaaga aaatagagca tggcgaaatt aaaatcaaaa ccttatcaaa gatgaccgat
     3841 gaggaagtaa tatcacatct cacacaagtt aagggaatag ggagatggac agcagaaatg
     3901 tttctaattt tttctttgga aagacaagac gtctttccag taggagatct tgggttaaga
     3961 aaaggtgtac aaaaggcctt ttcactaaag gaattaccaa acccaaaaga tgttg
//
LOCUS       DATIDL010000062        27169 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrosopumilaceae archaeon isolate SMAG_U16342
            k141_2698782, whole genome shotgun sequence.
ACCESSION   DATIDL010000062 DATIDL010000000
VERSION     DATIDL010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935836
            Sequence Read Archive: SRR25158326
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosopumilaceae archaeon (soil metagenome)
  ORGANISM  Nitrosopumilaceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrosopumilales;
            Nitrosopumilaceae.
REFERENCE   1  (bases 1 to 27169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/04/2024 05:09:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,520
            CDSs (total)                 :: 1,474
            Genes (coding)               :: 1,474
            CDSs (with protein)          :: 1,474
            Genes (RNA)                  :: 46
            rRNAs                        :: 1 (5S)
            complete rRNAs               :: 1 (5S)
            tRNAs                        :: 43
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27169
                     /organism="Nitrosopumilaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2698782"
                     /isolate="SMAG_U16342"
                     /isolation_source="soil"
                     /db_xref="taxon:2202732"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..276
                     /locus_tag="VLB45_02705"
     CDS             <1..276
                     /locus_tag="VLB45_02705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04644.1"
                     /translation="NKETEKTMTVSITPNKNTSLAVYDSGEYWIESQITTPNGVDNAY
                     TSIVIQNPSEKHALEFFESFSIPIKEIFIISAILAIATAVGLKFRRK"
     gene            complement(278..1885)
                     /gene="pckA"
                     /locus_tag="VLB45_02710"
     CDS             complement(278..1885)
                     /gene="pckA"
                     /locus_tag="VLB45_02710"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: protein motif:HMM:TIGR00224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HSD04645.1"
                     /translation="MIENSKSVITSKFVSQLETMGIRPAKVHRNLCVQTLVDLVVQKN
                     EGIITSTGSLSVKTGKYTGRSPDDRYIVDDAETRSKVDWGKVNHPFSEEKFEQLFQKM
                     KKHLEGKEIFVFDGFVGADPNNQISIRVINDHPWQNLFSRQMFIRPSTAELESHEPEF
                     TVLAINDFKAIPEIDGTRSDAFIILNFSRKLVLIGATSYAGEIKKSMFSVMNYVLPDR
                     GVFPMHCSANIGRNGDTALFFGLSGTGKTTLSADSNRMLIGDDEHGWSNKGVFNFEGG
                     CYAKCINLNRDAEPQIWDAIRFGAVLENVVIDKNTMKPDFNDGSITENTRVAYPLDYI
                     PGAVIPSLGGNPKVIVFLTADAFGVLPPIARLTKDGAMYHFMSGYTSKLAGTERGITE
                     PKATFSECFGAPFMSRHASVYAKMLGEKITQHNTTVYLINTGWSGGPYGIGKRINIKY
                     TRNMVTAALTGTLDAVKYRHDDIFNLDVPATCQDVPPEILDPRNTWVEKDAYDSSARK
                     LAQMFVENFKKFGETPKEIRDAGPHVS"
     gene            1994..2395
                     /locus_tag="VLB45_02715"
     CDS             1994..2395
                     /locus_tag="VLB45_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32e"
                     /protein_id="HSD04646.1"
                     /translation="MPINKEILELRRKASEHRPTFVREESWRYDRLAESWRKPKGIDN
                     HQRKQKKGWPRIVRAGFGSPKVSRGLHPSGYTDNLVYNTKDLEKLNPKTDGVRFGHGV
                     GKKKRLEIVAKAKEKNFKVFNARVSTSGSKS"
     gene            2379..2834
                     /locus_tag="VLB45_02720"
     CDS             2379..2834
                     /locus_tag="VLB45_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010191569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19e"
                     /protein_id="HSD04647.1"
                     /translation="MVVNLNTKRRLVSRILGVGANRVRFDSDHLDDVADAITRDNIRS
                     LFTANIISIKPIKGTSRGRAYFKKSQRHRRGTKQGSKKGSRGARVGKKEVYVKKMRSM
                     RHQIKVSKSRKEITNKDYWNLYKQVGGNQVRNLAHLRSLIQETQTKKHT"
     gene            complement(2831..3130)
                     /locus_tag="VLB45_02725"
     CDS             complement(2831..3130)
                     /locus_tag="VLB45_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HSD04648.1"
                     /translation="MKLEEKVYKKLLEVPPGKVTTYGELAKAVGLKNGQRVIGKIMNK
                     NPYPVIVPCHRVVKSDGNVGGYAWGEKVKTNMLTKEGIKIKNRKIMSMEETVYKF"
     gene            complement(3127..3903)
                     /locus_tag="VLB45_02730"
     CDS             complement(3127..3903)
                     /locus_tag="VLB45_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HSD04649.1"
                     /translation="MQLLVAYKSDPAGSNMAHFISQNMKQDGDVYRGTNFDLVVIPTP
                     AISADWLEEKYHYDGFVFLSKHASETGTLALTCHSTGNFSDALFGGFKKQVAIPHPHL
                     QKSYMQSLWKKRDSFEKFEITIEATHHGPTALDKPALFIEIGTTEKEWNDKELCKNVA
                     KIIVNVMSEPLKKSKVAICFGGTHYPKKFTESLIENEYALGTVMPKHALEFLDESLFS
                     HILKRNAEAKFALVDWNSLGKHKKKVVDFIKTTDLELIKV"
     gene            3943..4122
                     /locus_tag="VLB45_02735"
     CDS             3943..4122
                     /locus_tag="VLB45_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase SEC61 complex subunit gamma"
                     /protein_id="HSD04650.1"
                     /translation="MGNPKQLLRDMANTLKLARKSDKDDYMQHLRLVLLGIATVGTIG
                     FIIQFVFSVINFGMR"
     gene            4125..4574
                     /locus_tag="VLB45_02740"
     CDS             4125..4574
                     /locus_tag="VLB45_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007549776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor Spt5"
                     /protein_id="HSD04651.1"
                     /translation="MSQEKSHFFAVRTTGGQEKVVMGLLENRIKLKKLNIQAVLLLEN
                     LKGYIVVEAIDANAAFDALQGIRHIRGQLRGELQFKDIEGYLIKKSTVSELAVDKIVE
                     IIGGPFKGMKATITRVDHDKEEATVILLDAPYQLPVTVDANYLKLSA"
     gene            4612..5094
                     /locus_tag="VLB45_02745"
     CDS             4612..5094
                     /locus_tag="VLB45_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HSD04652.1"
                     /translation="MADIQTVSALVTGGQANAGPPLGPTLGPLGVNILQIVTAINDKT
                     KDFEGMKVPVTVTVDKGTKQWTVEVGIPSAAALILKEAGLQKGSGTSGATWVGDINFD
                     SIVKVAKLKIDSSYAQDIKAAAKEIVGTCLPLGIKIDGKNPKEVTAEINAGKWNEKLK
                     "
     gene            complement(5096..5530)
                     /locus_tag="VLB45_02750"
     CDS             complement(5096..5530)
                     /locus_tag="VLB45_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007549774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HSD04653.1"
                     /translation="MKILTELTRDGSVSVPNLSKKLGINTSVLYSRIKRLVKKKLITK
                     FTVIVDESLIGIGVRATMGINRDPKLKDQIHKALLKTPEVVVISEVTGRFDIIITVRA
                     KNLEELHTVAIEKIGKIEGIQNTETFVELQRTDKDPVYAVTK"
     gene            5725..6381
                     /locus_tag="VLB45_02755"
     CDS             5725..6381
                     /locus_tag="VLB45_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HSD04654.1"
                     /translation="MINETQLVQMVKEAKKEQKERKFKQSVELIMVFKDIDVKKGFAI
                     NETVQLPKKLSQPASVCVVASGDLGLKAKSANADRVVDGNEINKVGANKRESRKLING
                     YDFFLSDTQLMATVGKTLGQFMGPRGKMPTPVPFNAPIDSILERFRSSIRVRLRNSLS
                     LACKIGDETMADDDLVANANAVINLVEKKLPSGDKNIKKIMIKTTMGRIVKQPQVEKK
                     "
     gene            6383..7249
                     /locus_tag="VLB45_02760"
     CDS             6383..7249
                     /locus_tag="VLB45_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HSD04655.1"
                     /translation="MHQNRTKFPQKKTQMYQQLQELPKKYNVIALVRMEKVRSSQLLP
                     LRKKFKGEVEIISIKDKVAQKALAGLKIPAIEKVVDKLVGQCVLMFTNMSPFKLNILL
                     GKNKIMMAARGGDTASIDVVIKAGNTGITPGPILTDFKESGIATKIDQGTIWITKDST
                     VAKKGGVISAKLATLLSKLDVKPVEAGISLDSAVAEGTIYAKDEMVIDVEKYRNAFAQ
                     AHQEAVALSIEIGYVTKENIMLLLGKAAQGARSLSVEAGYLTDETKDQVLQKANSQAK
                     ALASKAKNYTPE"
     gene            complement(7278..7916)
                     /locus_tag="VLB45_02765"
     CDS             complement(7278..7916)
                     /locus_tag="VLB45_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007549771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HSD04656.1"
                     /translation="MPDLSLPIQILIYALDLFGTMAFAVTGAFKAIEHKADIVGIIIL
                     ATMTGVAGGTIRDVILGQFPPRSISDPWYVVIAISTGIVIFFIYPKIQKHWNLLLKFD
                     ALGLGVFTIIGATFAYNVFGLNFLAIALAGMLTAVGGGILRDVFVNEVPIVFVKELYA
                     SASFVGVLIFYLLLSMGIELTIVTIVGMIITTVLRLVAMKYNWNLPKARERL"
     gene            complement(7992..8294)
                     /gene="rpl12p"
                     /locus_tag="VLB45_02770"
     CDS             complement(7992..8294)
                     /gene="rpl12p"
                     /locus_tag="VLB45_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010189912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein P1"
                     /protein_id="HSD04657.1"
                     /translation="MEYVYAALLLHKQKKEINEANITNVIKASGAEVKEAQVKALVAA
                     LADVNIEEAIKAAPVAVAAAPAAPASGGKEDKQKEVETPSAKTEEQAMEGLSALFG"
     gene            complement(8366..8872)
                     /locus_tag="VLB45_02775"
     CDS             complement(8366..8872)
                     /locus_tag="VLB45_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04658.1"
                     /translation="MFGKKRWQEGDYVFSSQPDGEYKKIIVGIVTGVEDSKIGVNGII
                     INLVGLKNKVLQGKAGPKSMEILKNPDQTNCILGLIYRVEHENFTGVLDINSDSVVKI
                     HKRIHGIIDGWVRESLPELINNVLSLPDGPEKEQAKRILKQRMDTLYDKELKKYLYSV
                     CRSLKVLN"
     gene            8953..11655
                     /gene="alaS"
                     /locus_tag="VLB45_02780"
     CDS             8953..11655
                     /gene="alaS"
                     /locus_tag="VLB45_02780"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HSD04659.1"
                     /translation="MDKEQILSKFSADPNRYYKVSLFEDEGFVRKSCSKCKRFFWTID
                     HSQNFCPEDAENNYSFIGNPPTKKRFDYTQAWKEVESFFVQNGHKSISRYPVVCRWRD
                     DLYFTIASIVDFQRVMGSKVVFEFPANPLVVPQTCLRFKDLENVGVTGRHFSSFCMIG
                     QHSIPNSQGYWKDKCIELDFRLLTERFGIDKKEVVFVEDVWAGGGSFGSSLEYFVRGL
                     ELGNAVFTEFQGDLNNYSVLDQKIIDMGAGLERFAWITMGTPTAYDCCFGPITNNLIQ
                     KIGIEIDPKILSSYFTAVAKNLKGKDLGQVRKIAIKQAGIDDAKFNKMITPLEGIYLI
                     VDHLRTLVFAISDGALPSNVGGGYNLRMILRRVIATMDRLGWNLDLKDLVDMHVDYLK
                     STYPELEDTREEVKTIIGIERERYGESKIRMKSIASTIKSQSKKLSVDDLIRLYESDG
                     ITPDFLKETEIISEIPSTFYERLSDLHQSEKAEKKEILNLDGIPDTIPLYYKDDPVEF
                     EAKVLKVLNKKMVVLDRTSFYPRGGGQEPDYGKIENFDVIDVNKHGNVIVHELKDGTP
                     NEGTTVRCKVDSTRRYGITRNHTSTHVLNASARNTLGSWVWQHSAFKERDYGRLDITH
                     HSNLTEEDVAKIEELANLTIRKNVPIAINEYERGEAEQKFGFRIYQGGVVPVKSVRIV
                     NIEGFDVEACGGTHVKKTGEIGLIKITKTERIQDGVVRMEFVSGDTAIKYTQNQDRKI
                     SHIVKSLGSSKEKILESFEHAIEDSESAKKKLKQLIKRVSTSTAKSVIAEAKNLGKVR
                     LYTTVDEEMDEEFHIAVGEQVIKLDSHTVYCALILKNNGIRIVAFTGTDAAKIKKAGD
                     LVRDISKILGGSGGGNDSFGQGGGKDVSKIKDALLAAEQLVLAK"
     gene            11660..14539
                     /gene="leuS"
                     /locus_tag="VLB45_02785"
     CDS             11660..14539
                     /gene="leuS"
                     /locus_tag="VLB45_02785"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HSD04660.1"
                     /translation="MIDWSAIESKWQKKWHDEKHFEVEPNEKKKFFVTVAYPYPNSPQ
                     HVGHGRTYTLADVHARFMRMRGYNVLFPMGFHYTGTPILGMARRVQENDQELINAFRT
                     LYKVPEQNIKKFSEPVEIASYFHQEIKSGMIEMGYSIDWRREFTTIDPVYNKFIEWQF
                     RKLKSKNLVIQGSHPVGWCPKDQNPVSQHDTLGDVEPSFTEYVLIKFRYDDHILPTAT
                     LRPETIFGVTNLWINPSVTYKIADVDGESWIVSTECAKKLEFLDKKVKIVGEIKGSEL
                     VGKKVKMPERIDEITIFPASFVKSQNGTGIVMSVPAHAPFDYQALEDIKKNTNEIEQS
                     LLSIARSIHPISIIQTKEYGEIPAKEVVEKYKIENQNSPHLEEATKELYGKEFYGGRL
                     KQNTSQFAGKMVADAKDDVKKWILNNKNADILFELNDGPIRCRCGAECVVKILNNQWF
                     LDYSNQNWKNDAHSCINNMQILPEDIRNEFNHVVDWLRERACARQHGLGTKLPWDKDW
                     IVESLSDSVIYMAYYIIAKYVNKKQLMADNLKDEFFEYVFFGNGNLQKISSECNVSQE
                     IVEQIRNDFSYFYPVNSRHSGRDLVPNHLTFFVFNHIAIFPKKYWPEEIVVNGSVLMD
                     GKKMSKSMGNIIPLRDAIRTYGADPIRLAILISAELLQDADFNLEAVKGIKNKLENLL
                     EDCSKYKSNSNKNIEQEDRWLQSRLQQLVLEITTAMEKMRLREALHYVLYEFDSDIQW
                     YLKRINAKNRSDMPNVLHDLLSARVAMLSPFAPHVTEEMWEKLGNQGLASKAPWPSVH
                     SEKIDMLSIQSESLLKSTMEDIRNILKVTKMTPKKIIIYTSDIWKTKAYQKILASITN
                     GEMNIGVLIKSLISDKETEQVKKDPDFVKKTVNDILSESAESREAKTKTDILDEKKIL
                     SELDSLVQKEIGVKVQVFSESDQAKYDPKNKARTARPFKPAILIE"
     gene            14611..15618
                     /locus_tag="VLB45_02790"
     CDS             14611..15618
                     /locus_tag="VLB45_02790"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HSD04661.1"
                     /translation="MKIAIVGIGVAGGYLLARLKKEHEVVGFERSSQETHDSICAWGT
                     TKGPMQQFAQKCDLDFNDYVIHDGKNMYIDMNSERFNIKLMGLCTYDKIGLIKDMSKG
                     CKINYNTTPKLEDLESEFDLIIDATGFYRTYLPKPAMDFYLPTYQYKIEYEDQVPFDD
                     FYLKPFSSISGYFWYFPLGKKLAHIGAGDKNRKHVEETDSFLKKHGGKIIKTVGRPIR
                     LATPDMCEPFYHGKVVGVGESIGTVYPLLGEGIIPSMTCADIFVENLGNNERYRQQVL
                     EKFAIYPKVLSFVRNKMDSKFGMLKHFADLIAIYRYMKKNEKRFGMEIRMTDMMKVAR
                     A"
     gene            complement(15615..16319)
                     /locus_tag="VLB45_02795"
     CDS             complement(15615..16319)
                     /locus_tag="VLB45_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HSD04662.1"
                     /translation="MRKEIEDSLDLLAKDWDIDPIIREFVLGNRDDVSEYMIKVKDVV
                     FHVPYLQKEDSYILWKCYWPDCHNCCNRQGRLPLTSDDLITIGKSLKYQKTSDFIKNE
                     TNTATWEEGGPGGNMNTIMTMINLKRKKDELLKEDGTHISCRFLDDKGYCGLHPGRPG
                     VCYLYPFSSWLENERGRARVHATFQFTGDCPGFYLAKSIDQMKDVLLDYSKIIYDYNM
                     SSNRTVRENFGAVSLG"
     gene            16358..16678
                     /locus_tag="VLB45_02800"
     CDS             16358..16678
                     /locus_tag="VLB45_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04663.1"
                     /translation="MLSDKGKYASATQNRRLVWEKVMWPLILELNSVSFTMEQYKKKR
                     NEICKELEIPPSRIAGGFVSLLLKGILYKEKDLYSIHYRLIPYMRLKADCDYATAVRE
                     TTTK"
     gene            16710..16813
                     /locus_tag="VLB45_02805"
     tRNA            join(16710..16763,16779..16813)
                     /locus_tag="VLB45_02805"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:16760..16762,aa:Gln,seq:ctg)
     gene            complement(16841..17440)
                     /locus_tag="VLB45_02810"
     CDS             complement(16841..17440)
                     /locus_tag="VLB45_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008298130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HSD04664.1"
                     /translation="MAETANLLLFRKWDLSNIEIKDPGLKNAISVKKTIMPMTFGRSA
                     LKRFNKAEVNIVERLANKLLHFGKRYAKNTGRMGGKKTRSINTVKAAFEIMHLKTGKN
                     PIEVLVRAIENSAPNEDTTRIVYGGTVYHVSVDVAPLRRVDLALRFIAEGVRDASFSN
                     PKAIEENLAEHLILAAANDMNAPSVKKKNELERIAMASR"
     gene            complement(17445..17882)
                     /locus_tag="VLB45_02815"
     CDS             complement(17445..17882)
                     /locus_tag="VLB45_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HSD04665.1"
                     /translation="MAKSPLGLFSGRVLKDKRKKQRWAIGTYKRRNLGLDKKANPLGG
                     APQARGIVLEKVGIEAKQPNSAVRKCVRVQLIKNGKTVTAFLPKDGAMNFVDEHDEVH
                     VEGMGASMGGAMGDIPGVRWKVFKVNGTSLRELVYGKKEKPRR"
     gene            complement(17889..18347)
                     /locus_tag="VLB45_02820"
     CDS             complement(17889..18347)
                     /locus_tag="VLB45_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusA-like transcription termination
                     signal-binding factor"
                     /protein_id="HSD04666.1"
                     /translation="MTETIKLTTDQIKLMSLFQNVTGATARDCIEDEKLDRVIFVVNP
                     GKMGLAIGKGGVTIRNLQNVVKRNVELVEYAEDPADFLKNMLNPKLVSEVKINKRLDG
                     SMQAIVMVDAKKKGIVVGREGRNAEKARLLAKRYFQISSVLIQSPEKIME"
     gene            complement(18425..18745)
                     /locus_tag="VLB45_02825"
     CDS             complement(18425..18745)
                     /locus_tag="VLB45_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal L7Ae/L30e/S12e/Gadd45 family protein"
                     /protein_id="HSD04667.1"
                     /translation="MTKLLEKIIKEAMDDNKCLLGAKEVIGSMKNSKLVILSESIPQN
                     ILQKIQETAKTSKTSTLNFKGSSVALGRLCGTQFRISALSIKTLSETNLKSLLKEAEQ
                     ESQE"
     gene            complement(18738..22532)
                     /locus_tag="VLB45_02830"
     CDS             complement(18738..22532)
                     /locus_tag="VLB45_02830"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit A'"
                     /protein_id="HSD04668.1"
                     /translation="MSLETIKVIDGIRFSVWSPTEIRKYSVAEITAPETYDEDGMSVQ
                     GGLMDGRLGTLEPGQKCVTCGNTAARCPGHFGHIELAEPVLHIAFIDNIYKLLLTTCR
                     SCARLKISQEDLEQYHKIKSKEAAYTVISIKHIPDEIIEKAKKEKTCPHCGKSQYDLI
                     FTKPTIFIEKTELGENRLLPITIRERFSHMIDDDLKLLGYDPTTARPEWFVLQVLPVP
                     PVTVRPSIILETGIRSEDDLTHKLVDIIRVNQRLKESKEAGTPPLIVQDLVDLLQYHT
                     TTYFDNEVSGIPQAHHRSGRPLKTLTQRLKGKEGRFRGSLSGKRVDFSSRTVISPDPN
                     LDISEVGVPESVAIKLTIPEVVTDWNIERLKKLVINGPYKFPGANYIVRPDGVKIRLD
                     FVEDRETIANNIEIGYLIERHLSDGDIVMFNRQPSLHQMSIMAHYVKVLPYKTFRLHP
                     SVCPPYNADFDGDEMNLHVPQSEEARAEAILLMRVQDQLISPRYGGPIIGALRDFITG
                     AYLLTKDGTTLTPQEFANLAMIAGYDGTFPEPAVKNKTGGLYTGKQLFSLFMPKDFNY
                     VITSKWSKGTKGPAKDVVIKNGQLISGVIDKASIGAEEPDSVLHRITKDYGYEEAKKF
                     LNSILIMLKQFITHYGFSYGYSDLELSQKTKENIMVGIQETYDKVYDLISQAKKGTLQ
                     LSRGLSADEALEAYIVNELSKARDRAGSTADKSFDETNSGRIMATTGARGSSLNIGQM
                     AGALGQQSIRGKRIQKGYNNRALPHYKENDTNPDARGFVKSNYREGLSTLEFFFHAMG
                     GREGLVDTAVRTQQSGYMQRRLINALEHLKLEYDGTVRDPHGHIIQFLYGEDGIDVAK
                     SDHGEAFRIARLVESEAIIDSGKKATKEEIADLAKKYTKTFNPRLTQTVQEALFESKL
                     SKEGVEKVSKKALELYEKAKVEPGQAVGIVTAQSIGEPGTQMTLRTFHFAGIRERNVT
                     LGLPRLIELVDARKKPVTPTMDIYLEPKFKKSKEKAIQVAREILHTKISALISSTETD
                     YSSKIKLNLNTDKLRERACTVADVEEALQSSKKKFEIEPSGNSITLTLAEESDAQTVL
                     ALRNKVLGTKVKGVADVERVTIVQQNDEWVIQTSGSNIAKVLEIEGIDKKNIRTNNVF
                     EIANTLGIEAARNALINELLSTLEDQGLEVDVRYIMLVADLMCSKGYLQQIGRHGIAG
                     TKTSVLARAAFEITVPTIADAALAGEIEHLKGVTENVIVGANIPIGTGTVDLYMKVVQ
                     DD"
     gene            complement(22532..25879)
                     /locus_tag="VLB45_02835"
     CDS             complement(22532..25879)
                     /locus_tag="VLB45_02835"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010189656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit B"
                     /protein_id="HSD04669.1"
                     /translation="MVNISNKRWPIIQDILKREGIARQHLNSYDEFLERGLQSIIDEV
                     GQIEIESAEYPYKIQLGKLKLQQARMMELDGSITHIAPMEARLRNVTYASPVMLEASV
                     VEDGKILESRFVHIGDMPVMVRSNACILHNLSDQKLVEHGEDPNDPGGYFIINGSERV
                     IVGLEDLSYNKIIVDKETVGGNTVFKAKVYSSIVGYRAKLELVMKNDGLIVAKIPGSP
                     VDIPVVTLMRALGLESDREIAAAVSLVNDIQDELEASFEKSGDVPTAKDAIVYISKRI
                     APGMLEEFQIKRAETLLDWGLLPHLGKNPDNRKEKAQFLGEAACKLIELKLGWLTPDD
                     KDHYGNKVIKFAGQMLADLFRTAFRNLVRDMKYQLERSGQKRGINAVAAAVRPGIVTD
                     KLNNAIATGNWGRGRVGVTQLLDRTNYLSTISHLRRIQSPLSRSQPNFEARDLHATHF
                     GRICPSETPEGSNCGLVKNLALSAIISVNVPSEDIIEKLYDLGVTYVSDAKDDLKKEG
                     SRVFVDGRLIGYYKDGQKLTDSLRDLRRSFKIHPHIGIFLYQSNIEGSTKRLYVNCNA
                     GRVLRPLIVIKDNKPLLTQELIDKVSKKFLSWNDLLHMGVIELVDANEEENCYIAMDE
                     ENLKKHTHMEIFPSAILGAGASIIPYPEHNQSPRNTYESAMAKQSLGFSTPLMNASTY
                     VRQHFMLYPQTPIVTTKAMGLLGLEDRPAGQNCVVAVLPFDGYNIEDAIVLSRSSVER
                     GLGRTFFYRIYEAEAKQYPGGMRDNFEIPNAEGNIRGFRGDKAYRLLEEDGVIATESP
                     VQGGDILIGKTSPPRFMEEYREFEVKGPYRRDTSIGVRPSENGVVDTVVMTQSHDGGK
                     MYKIRVRDVRIPEIGDKFASRHGQKGVVGMLVNHEDLPYTSDGVVPDVLINPHAFPSR
                     MTVGMFLESVTGKAAALRGTKMDGSAFVGEKLDDVKGVLESAGFKYSGKEIMYDGRTG
                     KAFEAEVFIGVVYYQKLHHMVADKIHARARGQVQMLTKQPTEGRARGGGLRFGEMERD
                     CLIAYGASMMLKDRLLDESDKADIYICERCGLVSYYDIKQRRFVCRVCGDKAKVTSIS
                     VAYAFKLLLQEMMSLDVAPRLLIKERV"
     gene            complement(25880..26131)
                     /locus_tag="VLB45_02840"
     CDS             complement(25880..26131)
                     /locus_tag="VLB45_02840"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit H"
                     /protein_id="HSD04670.1"
                     /translation="MLTKKDHILVPDHIYVPKHEIMTKKEAEEVLEQFHTKPTEMPLI
                     YENDPAIKGLGVKPGDMIKITRKSATSGESVYYRYVVEE"
     gene            26309..27136
                     /locus_tag="VLB45_02845"
     CDS             26309..27136
                     /locus_tag="VLB45_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEFG-CTERM sorting domain-containing protein"
                     /protein_id="HSD04671.1"
                     /translation="MSTRFKGFSLLAILVAAIGAVPAFAQVDPFDTVPITVTTDQESY
                     ADGDTIVISGATKDYISGQQVSIVVINPIGNIVTVQQVDLETDKTYSTVLTAGGGLWK
                     AAGTYTVKVQYGGENRANETTFEFSGSAGGPGGKKIHVEGTDFDVRYSITGGKVLGIT
                     ADVEAKSLLVSIETTSDGVLTITLPRALIDAKINGEDDSFFVLIDGEEIGDVSEETTS
                     TDRTLTIPFTDGSEEIEIIGTQIVPEFGAVAALILAVAIISIIAVSAKTRLRLMPKY"
BASE COUNT         8472 a         5045 c         5089 g         8563 t
ORIGIN      
        1 aataaagaaa cagaaaaaac tatgactgtg tccatcacac ctaacaaaaa cacatcactt
       61 gcagtgtatg attcaggtga atattggatc gaatcacaga taactactcc aaatggggtt
      121 gataatgcat atacaagcat agtgattcaa aatccatcag aaaaacatgc gttggaattt
      181 tttgaatcgt ttagcatacc aataaaagag atttttataa tttctgcaat acttgcaata
      241 gcaacagcag tgggattaaa attcagacgc aaatagttta ggatacgtgc ggtcctgcat
      301 cccgtatttc ttttggagtt tctccaaatt tcttaaagtt ctctacaaac atttgtgcga
      361 gttttcttgc agacgaatca tatgcatctt tttctaccca cgtatttcgt gggtctaata
      421 tctctggagg cacatcctga catgttgcag gtacatcaag attgaatatg tcatcatgcc
      481 tatactttac agcgtcaagt gtgcctgtaa gagctgcagt aaccatgttt ctggtatatt
      541 tgatgttgat tctcttacca atcccatatg ggccgccaga ccatcctgtg tttatcagat
      601 aaacggtagt gttgtgttgg gtaatttttt ctccaagcat ctttgcatat actgatgcat
      661 gacgcgacat gaatggagcc ccaaaacatt cagaaaatgt agcttttggt tctgtgattc
      721 ctctttctgt acctgcaagc ttgctggtat agcctgacat aaaatgatac atggcaccgt
      781 cttttgtcag tctagctatt ggaggaagaa caccaaatgc atctgctgtc agaaacacaa
      841 tcacttttgg atttcctccc aagcttggaa tgacagctcc tggaatgtaa tcaagtggat
      901 atgcgactct ggtattttca gtgatgctgc catcgttgaa atctggtttc atggtgtttt
      961 tgtctatcac tacgttttcc aaaacagcac caaaccttat tgcgtcccat atctgcggtt
     1021 ctgcatcacg attcagattg atacactttg cataacatcc tccttcaaaa ttaaagacgc
     1081 ccttgtttga ccaaccatgt tcgtcatctc ctatcagcat cctgtttgaa tctgccgaaa
     1141 gtgtggtttt gcctgttcct gagagtccaa agaataacgc agtgtcgcca ttcctgccaa
     1201 tgtttgcaga gcaatgcatt ggaaacactc cacgatctgg taaaacataa ttcataacag
     1261 agaacatgga tttttttatt tctcctgcat atgacgttgc tccaataagt acaagttttc
     1321 ttgaaaagtt tagtattatg aatgcatcag aacgtgttcc atcaatttca ggaattgctt
     1381 tgaaatcatt tattgcaagc actgtaaatt ctggttcatg cgactctaat tctgctgtgg
     1441 acggtcttat gaacatctgt ctagaaaaga gattttgcca cggatgatca tttatgactc
     1501 gtattgatat ctgattatta ggatctgcac caacaaaccc gtcaaagaca aagatctctt
     1561 ttccttcaag atgctttttc attttttgaa agagttgttc aaatttttct tcagaaaatg
     1621 ggtggtttac tttaccccag tctactttgc tacgagtttc agcatcatca actatgtaac
     1681 gatcgtcagg agaccttcct gtgtattttc ctgtcttaac tgaaagcgaa ccagtagagg
     1741 tgataatacc ctcgtttttt tgcactacca agtccacaag tgtctgcacg cacaaattcc
     1801 tgtgcacttt ggcaggtctg attcccatgg tttctagttg agagacaaat ttgcttgtga
     1861 ttactgattt cgagttctca atcatgagat aaaacaccgc accccatttc cttattatct
     1921 cttactgcaa tcagagacgc gtattttatt accagaaacg tatttatttt aaaattcgag
     1981 cagagtcatt tacatgccga tcaataaaga gatcctagaa ctgagaagaa aagcatcaga
     2041 acatagacct acttttgtca gggaagaaag ttggagatat gatagacttg cagagtcttg
     2101 gagaaaacca aaaggaattg ataaccatca gcgaaagcaa aaaaaaggtt ggccaagaat
     2161 tgtacgagct ggttttggca gtccaaaggt ttcaagagga ttacacccgt ctggatatac
     2221 tgataattta gtttacaata caaaagactt ggaaaaactt aatccaaaaa ctgatggtgt
     2281 aagatttggc cacggagttg gaaaaaagaa aagactcgag atcgtagcaa aagccaagga
     2341 aaaaaacttc aaagtgttta acgcgagagt gtcaacaagt ggtagtaaat cttaatacaa
     2401 aaagaagact agtctcaaga attttgggag taggagcaaa tcgagtcaga tttgattccg
     2461 atcatctaga tgatgtggct gatgcaatta cacgagacaa catacgaagt ctgtttactg
     2521 caaacataat atcaataaaa ccaataaagg gaacatctcg tggcagagca tattttaaaa
     2581 aatctcaaag acacagacgt ggaactaagc aaggctcaaa aaaaggaagc aggggagctc
     2641 gtgtgggtaa gaaggaagtg tatgtaaaga aaatgagatc tatgcgtcat cagatcaaag
     2701 tttcaaaatc aagaaaggag attacaaaca aggattactg gaatttatac aagcaagtag
     2761 gcggaaacca agtcagaaac ttggcacacc ttagaagcct aattcaagaa actcaaacaa
     2821 agaaacacac ttagaatttg tagacagttt cttccattga cataattttt ctattcttaa
     2881 ttttaattcc ctcttttgtt agcatgttag ttttgacttt ttcaccccat gcatatcctc
     2941 ctacgttacc atcagatttg acaacgcgat gacaaggaac tattactgga tagggatttt
     3001 tgttcataat tttaccgatc actctttgtc catttttaag accgactgct tttgcaagct
     3061 caccgtaggt agttactttt cctggtggaa cttcaagaag ttttttatag actttttctt
     3121 ctagtttcat acctttatca actctagatc tgttgttttt ataaaatcta ctactttttt
     3181 cttatgtttt cccaagctat tccaatccac caatgcgaat ttggcttcgg catttctttt
     3241 taatatgtga gaaaataaag attcatctag aaattctaaa gcatgttttg gcattacagt
     3301 accaagcgca tattcatttt ctatcaaaga ttcagtgaat ttttttggat agtgtgtacc
     3361 gccaaaacat atggctactt ttgatttttt tagtggttca gacatgacat tgactatgat
     3421 tttagccacg tttttgcata attccttatc attccattct ttttctgtag taccaatctc
     3481 aatgaataga gccggtttgt ccagagcagt aggtccatga tgtgtagctt caattgtgat
     3541 ttcaaatttt tcaaaactat ctcttttttt ccataaagat tgcatgtatg acttttgtaa
     3601 atgaggatgt ggaattgcta cctgtttttt aaatccacca aaaagagcat ctgaaaaatt
     3661 tccagtacta tgacaagtga gcgcaagagt accagtttca gatgcatgtt ttgataaaaa
     3721 tacaaaacca tcgtaatgat atttttcttc aagccagtct gcagatattg ctggtgttgg
     3781 tatcacaaca agatcaaaat ttgttccgcg gtaaacatct ccatcctgtt tcatattttg
     3841 tgatatgaaa tgagccatgt tagaaccagc aggatctgat ttgtacgcaa caagcaactg
     3901 cacaagataa gagatataag tacactttat taacgtcctc caatgggcaa tccaaaacag
     3961 ctgttaaggg atatggcaaa cactctgaag cttgccagaa agtcagacaa ggatgactat
     4021 atgcaacatc tcagactagt actacttgga attgccacag ttggcacgat tggatttata
     4081 attcagtttg tgttttcagt aattaatttc ggaatgagat agaattgtct caggaaaaat
     4141 cacacttttt tgcagtaaga acaactggag gacaggaaaa ggtagtcatg ggacttttgg
     4201 aaaacaggat aaaattaaag aagctcaaca tacaggcagt gttactttta gaaaatctca
     4261 agggatatat cgtagttgag gccatagacg caaacgctgc ttttgatgca ctgcaaggaa
     4321 taagacacat acgcggtcag cttcgaggag aacttcaatt caaggacatt gaaggttact
     4381 tgatcaagaa atctactgtt tctgagcttg ctgtcgataa gattgttgag ataatcggag
     4441 gaccgtttaa gggcatgaag gcaaccatta ccagagtaga tcatgataaa gaggaagcaa
     4501 cagtgatttt gcttgatgca ccataccaac ttccagtaac tgtcgatgca aattacctaa
     4561 agctttcagc gtagcaagga ttaaatttac agctacaagg aaagaaaaaa catggcagat
     4621 atacaaacag tatcagcact tgtaacaggt ggacaagcaa atgcaggtcc gccattaggt
     4681 cctacacttg gtccacttgg cgtaaacatt ttacaaattg taactgcaat taatgataaa
     4741 acaaaagatt ttgaaggaat gaaagttccg gtcacagtca cagttgacaa gggaactaaa
     4801 caatggacag ttgaggtagg cattccatct gcagccgctc ttattttaaa agaagcaggt
     4861 ttacagaaag gttctggaac ttctggagca acttgggtag gagacatcaa ttttgattcc
     4921 atagttaaag tcgccaaact caagatagat tcgtcttatg cacaggacat aaaggctgcc
     4981 gcaaaagaaa ttgttggcac ttgtttgcca cttggaatta aaatagatgg caagaatcca
     5041 aaagaagtta ccgcagaaat taatgccgga aaatggaacg agaaattaaa ataggttatt
     5101 tagtaactgc ataaaccgga tccttatcag ttctttgtag ttctacaaac gtctcagtat
     5161 tctgaatccc ctcaatctta cctatttttt ctattgctac agtatgcagc tcttccaagt
     5221 ttttggcacg aactgtaatt ataatatcaa atctacctgt tacttcagaa atcactacta
     5281 cctctggggt ttttaatagg gccttatgga tttgatcctt tagttttgga tcacgattta
     5341 ttcccatggt tgcacgaaca cctataccaa taagagattc atctactatt acggtgaatt
     5401 tggttatcag tttctttttt acaagtctct ttattctact atacagaact gatgtgttaa
     5461 taccaagttt ttttgagagg ttaggaacag agacacttcc atcccttgtg agttcagtaa
     5521 ggattttcat gtcaagttca tcaaatttgt gcaaggctgt gcgagatagt tttagttacg
     5581 atttaataaa tgtaagttta acctctagaa aattttgttc ttacaacata atttgaataa
     5641 catgaaattt ttagacgaac gtcatatttt tgtgatttta gcctgtaaac gattttaaat
     5701 aggcaatcca gaaaaaatgc gtaaatgatt aatgaaaccc agctagtgca gatggtcaaa
     5761 gaggctaaaa aggagcagaa agaacgcaag tttaaacagt ctgtagaatt gataatggtt
     5821 ttcaaagata ttgatgttaa gaagggattt gccataaatg aaacagttca attaccaaaa
     5881 aagcttagtc aaccagcttc tgtctgtgtt gtagcttccg gagatctggg tttgaaagca
     5941 aaaagtgcaa atgctgacag ggtagttgat ggaaatgaaa taaacaaagt tggtgcaaac
     6001 aagcgtgaat cgcgcaagct aataaacggt tacgactttt tcttatcaga tactcaactt
     6061 atggctacag tgggtaagac tctaggtcag tttatgggtc caagaggcaa gatgccaact
     6121 ccagtaccat tcaatgcacc aatagattcc attttggaaa gatttcgatc ttccatacga
     6181 gtaagactaa gaaattccct atctcttgca tgtaagattg gtgatgaaac tatggccgat
     6241 gatgatttag tagcaaatgc caatgcagta ataaacttag tagaaaagaa gcttccgagt
     6301 ggagataaga acattaaaaa aatcatgata aaaacaacga tgggaagaat agtcaaacaa
     6361 ccacaggtag aaaagaagta atatgcatca aaatagaacc aaatttccac aaaagaaaac
     6421 tcagatgtac caacaactac aggagcttcc aaaaaagtat aatgtcatag cattagtaag
     6481 aatggaaaag gttagatctt cgcagttatt gcctttgaga aaaaaattca aaggcgaagt
     6541 tgagatcata agcataaagg ataaagttgc acaaaaagcc cttgcaggat tgaaaattcc
     6601 agcaattgaa aaagtcgttg acaagttagt tggccagtgc gttttgatgt ttactaacat
     6661 gagtccattt aagctaaaca tactacttgg taaaaataaa ataatgatgg ctgcgcgtgg
     6721 aggagacact gccagtatag atgtagtcat aaaagcagga aataccggta ttacaccagg
     6781 tccaattctt actgatttta aagaatcagg cattgccacc aaaattgatc aaggaacaat
     6841 ctggataaca aaagattcta cagtagcaaa aaaaggtggt gtgatttctg ccaagcttgc
     6901 cacattattg agcaagttgg atgtcaaacc agttgaagca ggaatatcac tagattcagc
     6961 agtagctgaa ggcaccatat acgcaaagga tgagatggtc atagatgttg aaaagtatcg
     7021 caatgccttt gcacaagctc atcaggaagc ggttgcactc tccatagaga tcggttatgt
     7081 aacaaaagaa aacatcatgc ttttacttgg aaaagcagca caaggagcac gatctctttc
     7141 agtggaagca ggatatctta cagatgaaac aaaggatcag gtgctacaaa aagccaattc
     7201 acaagcaaaa gcacttgctt caaaagcgaa gaattacact ccagagtagt ataaaatttt
     7261 ctgaaaaaat aatctgttca gagtctttct cttgcttttg gcagattcca gttgtatttc
     7321 atggcaacaa gtcgtagcac tgtagttatt atcataccca ctattgttac gatggttaat
     7381 tctattccca tactaagtag caagtaaaag atcaaaacgc caacaaagct tgcagaagca
     7441 taaagttcct ttacaaagac tattggcacc tcgtttacaa atacgtcacg taaaattcct
     7501 ccacctacag cagtaagcat cccagctagt gcaatggcta gaaagtttaa tccaaataca
     7561 ttgtatgcaa aagtggcacc tattattgta aatacaccca aacccagagc gtcaaatttt
     7621 aaaaggagat tccagtgttt ttgtattttt gggtaaatga aaaagattac aattcctgta
     7681 gagatggcaa ttacaacgta ccaaggatct gagatagagc gaggcggaaa ttgacctaat
     7741 ataacatcac gaatagtacc tccagccaca cccgtcatag tggccagaat tataattcca
     7801 acaatgtccg ctttgtgttc aatagctttg aatgcaccag taactgcaaa tgccattgtc
     7861 ccaaaaagat ctaatgcata aatgagaatt tgtataggta gtgatagatc tggcaaacta
     7921 caacctattg ttagtaatgg taaataatgt ttagagaatt aaatcaaaaa agtgttgtaa
     7981 aaaatatgtt tctagccaaa cagtgcagag aggccttcca ttgcttgctc ttctgtcttt
     8041 gcggatggag tttcaacctc tttttgttta tcttcttttc caccagatgc aggtgctgca
     8101 ggtgctgcgg caacagctac tggggctgcc ttgattgctt cttctatatt cacatcagct
     8161 aaagcagcaa caagtgcttt tacttgggct tcctttactt ctgctccaga agctttgatt
     8221 acatttgtaa tgttagcctc attgatctct tttttctgct tgtgtaatag tagtgcagcg
     8281 tatacgtatt ccattgtaag cgatttttgc tttaggcata atataaaact atggagcgga
     8341 ttctgaaaat cagaagcaga tcaagtcagt ttagaacctt tagacttctg catacagaat
     8401 aaagatattt ttttagttcc ttatcgtata gagtatccat tcgctgtttt agaattcttt
     8461 ttgcttgttc tttttcaggc ccatcaggaa gtgaaagtac attgtttatc agttctggaa
     8521 gcgattctct aacccatcca tcaatgattc catgtattct tttgtgtatt tttactactg
     8581 aatcagagtt aatgtctagc actcctgtga aattttcatg ctccacacga tagattaagc
     8641 caagtatgca attggtctga tcaggatttt ttaaaatctc catagacttt ggtccagctt
     8701 taccttgtaa gactttgttt tttaagccaa cgagatttat tatgattcca ttaacaccga
     8761 ttttagaatc ttctactcct gtcactatac ctacaattat cttcttatac tccccatcag
     8821 gttgtgatga aaagacgtag tctccttcct gccatctctt tttgccaaac ataataaatt
     8881 ttcaagaatg atcatattta atgtgtttcc tcagaatgct taatatcagt caaatcgaaa
     8941 actgtttcaa agatggacaa agaacaaatt ctttcaaagt tttctgcaga tcctaatagg
     9001 tattacaaag tatccttgtt tgaagatgag ggatttgtac gtaaatcttg ttcaaaatgt
     9061 aagcgatttt tttggacaat tgatcattca cagaactttt gtccagaaga tgcagaaaat
     9121 aattattctt tcataggaaa tcctcccaca aaaaagagat tcgattatac tcaagcatgg
     9181 aaagaagtag agtctttctt tgtacaaaat ggacacaaat caatcagtag gtatccagta
     9241 gtttgtagat ggcgtgacga tttgtatttt acaattgctt ctattgtgga ctttcaaaga
     9301 gtaatgggtt ccaaagttgt tttcgaattc ccggcaaatc cattagttgt accacaaact
     9361 tgtcttagat tcaaggatct tgaaaatgta ggagttactg ggagacactt ctcaagtttt
     9421 tgcatgattg gtcaacacag cataccaaat tcgcaaggat attggaaaga taaatgcatt
     9481 gagctagatt ttagactttt aactgagagg ttcggaattg ataaaaaaga agtggttttt
     9541 gtagaggacg tatgggcagg cggaggttca tttggctcat cattagaata ttttgtacga
     9601 ggattagaac ttggcaatgc tgtatttacc gaatttcaag gagatttgaa caactattca
     9661 gtcttggatc aaaaaataat agacatggga gcaggtctag aaagatttgc gtggataacc
     9721 atgggaactc ctacagcata tgattgctgt tttggtccaa taacaaacaa tttgatacaa
     9781 aaaattggaa tagaaataga tcctaaaata ttatcatcat atttcactgc agtagcaaag
     9841 aatcttaaag gaaaagatct tggccaagtc agaaaaatag caataaaaca agctggcata
     9901 gatgatgcaa aattcaataa gatgataacg cctcttgagg gaatctacct gattgttgat
     9961 cacttgagaa cgctagtatt tgcaatatca gacggagcgt taccaagtaa tgttggtgga
    10021 ggttacaatt tgagaatgat tttgagaagg gtgatagcca cgatggatag actgggttgg
    10081 aatcttgatc taaaagatct agtcgatatg catgttgatt atctaaaatc aacgtatcct
    10141 gaactagaag atacccgtga agaggtaaag acgataattg ggatagaaag agaaagatat
    10201 ggtgaatcaa aaatcagaat gaagagcatt gcaagtacaa taaagagtca aagcaaaaag
    10261 ctatcagttg atgatttaat cagactatac gaatcagatg gaataacacc agacttccta
    10321 aaagagacag agataatttc agagatccca tcaacatttt atgaaagatt atcagatttg
    10381 catcaatcag aaaaagcaga aaaaaaagaa attttgaatt tggatggaat tccagacaca
    10441 attccgttgt attacaagga tgatccagtt gaatttgaag ccaaagttct caaagtgttg
    10501 aacaagaaaa tggttgtttt ggacagaacc tcgttttatc caagaggtgg agggcaagaa
    10561 ccagattatg gaaaaataga gaattttgat gtaatagatg tgaataagca tggaaatgta
    10621 atagtacatg agttaaagga tggaacccca aatgagggaa ccacagtaag atgcaaagtt
    10681 gattccactc gtcgttatgg aataacaaga aatcatacaa gtactcatgt attgaatgca
    10741 tcagcaagaa atactctagg atcgtgggtg tggcaacatt cagcattcaa agaaagagat
    10801 tatggcagac ttgacataac acatcattca aacctgacag aagaagatgt tgcaaaaata
    10861 gaagagttag caaatctcac tattagaaaa aatgtaccca ttgcgataaa tgaatatgaa
    10921 agaggtgaag cagaacagaa atttgggttc cgcatatatc agggaggagt agttcccgtt
    10981 aaatcagtaa ggattgtaaa cattgaaggt tttgatgtcg aggcatgtgg tggcacgcat
    11041 gtaaaaaaga caggcgaaat tggattaata aaaataacaa aaacagagag aatacaggat
    11101 ggagtcgtaa gaatggagtt tgtttcagga gatacagcta taaagtatac acaaaatcaa
    11161 gaccggaaaa tatctcatat tgtcaaatca ttaggctcta gcaaggaaaa aattttagaa
    11221 tcatttgagc atgcaataga agattcagaa tcagcaaaga aaaagctaaa acagttaatc
    11281 aaacgagttt ctacttccac tgcaaaaagt gtaattgcag aagcaaaaaa tctcggaaaa
    11341 gtgagattat acaccacagt agacgaggaa atggatgaag agtttcatat tgcggtaggc
    11401 gaacaagtca taaaattaga ttcacacact gtatactgtg ccttgatttt aaaaaataac
    11461 ggaataagga tagtagcatt tacaggtacg gatgctgcaa aaatcaaaaa agcaggagat
    11521 ttggtgcgag acatatcaaa aattctaggc ggttctgggg gaggaaatga tagctttggt
    11581 caggggggcg gaaaggacgt ttccaagatc aaagatgcac ttcttgctgc agaacaatta
    11641 gttttggcaa agtgattatt tgatagattg gtcagcgata gaatctaagt ggcaaaaaaa
    11701 atggcatgat gagaaacatt ttgaggttga gcctaatgaa aagaaaaaat tctttgtaac
    11761 cgtagcttac ccatatccca actcgccaca acatgtagga catggaagaa cttacacttt
    11821 agcagatgtc catgccagat tcatgagaat gcgtggatac aacgtactct ttccaatggg
    11881 atttcattac actggaaccc caatcctcgg aatggcaaga cgtgtgcaag aaaatgatca
    11941 ggaattgatt aatgccttta gaactttgta caaggtacca gaacaaaaca taaagaaatt
    12001 ttcagagccg gtagagatcg caagttattt tcatcaagaa ataaaatctg gcatgataga
    12061 gatgggatat tccatagatt ggcgaagaga gtttacaaca attgatcctg tatacaataa
    12121 attcattgag tggcaattta gaaaattaaa aagtaagaat ttagtcatac agggttccca
    12181 tccagtagga tggtgtccaa aagatcagaa cccagtatca caacatgaca cgctgggaga
    12241 tgtagaacca agctttacag aatatgtcct cataaagttc aggtatgacg accatatctt
    12301 gcctactgca acactcagac cagaaacaat atttggcgtc actaatcttt ggataaatcc
    12361 cagtgtcaca tacaagattg ctgatgtgga tggtgaaagt tggattgtta gtacagaatg
    12421 tgcaaaaaaa ctagaatttt tggataagaa agtcaagatt gtaggcgaga tcaagggttc
    12481 agaacttgta ggcaagaaag tgaaaatgcc agagaggata gatgagatta caatatttcc
    12541 agcaagtttt gtaaaaagtc agaatggcac agggatagta atgtcagtcc cagcacatgc
    12601 accatttgat taccaagcct tggaagatat taagaaaaat acaaatgaaa ttgaacaaag
    12661 ccttctgtcc atagcaagat cgattcatcc catatcaata atacagacta aagaatatgg
    12721 agaaattcct gctaaagaag ttgtagaaaa atataaaatt gaaaatcaaa atagtccaca
    12781 tcttgaagag gccacaaaag agctgtatgg gaaggaattc tatggtggaa gactaaaaca
    12841 aaatacaagt cagtttgcag gaaaaatggt agctgatgca aaagacgatg taaagaaatg
    12901 gattttgaat aacaagaatg cggacatttt gtttgaatta aacgatggac ctatcagatg
    12961 cagatgcggc gcagagtgtg ttgtcaagat attaaataat caatggtttt tggattattc
    13021 taaccagaac tggaaaaacg atgcgcattc atgtattaac aacatgcaga tcttaccaga
    13081 agacatacga aatgaattca atcatgtcgt tgactggcta cgtgaaaggg catgtgcaag
    13141 acaacacggc ttaggaacaa agcttccatg ggataaggac tggatagtag aaagcctctc
    13201 agattcggta atttacatgg catattatat catagcaaaa tacgtaaaca aaaaacaact
    13261 catggctgac aatttaaaag atgaattttt tgaatatgta ttttttggaa atgggaattt
    13321 acagaaaatt tcttccgaat gcaatgtctc ccaagagatt gttgaacaga taagaaatga
    13381 cttttcatat ttttatcccg taaattctag gcattcgggc agagatttgg tgcctaatca
    13441 tttaacattt tttgttttca accatattgc aatttttcct aaaaaatact ggccagaaga
    13501 aattgtggta aacggttcag tattgatgga tggaaaaaag atgtcaaaat ccatgggaaa
    13561 tataataccg cttcgtgatg caatacgaac ttatggtgcc gatccaatca gattagcaat
    13621 attaatttct gcagagctct tgcaggatgc cgattttaat ttggaagctg taaaaggaat
    13681 taaaaataag cttgaaaatt tgttagaaga ttgttccaaa tacaaatcaa attctaataa
    13741 aaatatagaa caagaagaca ggtggcttca aagtagatta caacaactgg tgttggagat
    13801 tactacagct atggaaaaaa tgagattgcg tgaagcttta cattatgttt tgtatgaatt
    13861 tgattcagac atacagtggt atctaaagag aattaatgca aagaacagat cagacatgcc
    13921 aaatgtatta catgatttgc tttctgcaag agtagcaatg ctctcaccat ttgctcccca
    13981 cgtaacagaa gagatgtggg aaaagcttgg aaaccaagga ttagcttcaa aggcaccttg
    14041 gccatctgtg catagtgaaa aaatcgacat gttatcaatt caatcagaaa gtcttctcaa
    14101 gtcaactatg gaagacataa gaaacatact aaaagtaaca aaaatgacac caaagaaaat
    14161 tatcatatat acttctgaca tatggaagac aaaggcctac cagaagatcc ttgccagcat
    14221 aactaatgga gagatgaata taggagtttt aataaaatct cttatttccg ataaagagac
    14281 agaacaagta aaaaaagatc cagactttgt caagaaaacc gtcaatgaca tactatctga
    14341 atctgcagag tcacgagaag caaaaacaaa aactgacatt ttggatgaga aaaagattct
    14401 ttccgaatta gattctttgg tgcaaaaaga aattggtgtg aaagttcagg tattttcaga
    14461 atctgatcaa gcaaagtatg atccaaagaa caaggctaga accgcaagac catttaaacc
    14521 tgccatatta atcgaataaa atttacaatt caaacaggat tcagcagatc aagtattact
    14581 tttattaggt attttcagat tagaaattaa ttgaaaattg caattgtagg cataggtgtt
    14641 gctggcggat atcttcttgc cagattaaaa aaagagcatg aggtagttgg atttgaaaga
    14701 tcgtcgcaag aaactcatga ttccatatgt gcatggggta caacaaaagg cccaatgcag
    14761 caatttgcgc agaagtgtga tcttgatttt aatgattatg tgatacatga cggaaaaaac
    14821 atgtacattg acatgaattc tgaaagattc aacataaagc tgatgggctt gtgcacatat
    14881 gataagattg gactcataaa ggacatgtca aagggatgca agattaatta taacacaaca
    14941 ccaaagctcg aagatttaga atcagaattt gatctgataa tagatgcaac tggtttttac
    15001 agaacgtatc ttccaaaacc tgctatggat ttttatcttc caacttacca gtacaaaata
    15061 gaatacgaag atcaggttcc ttttgatgat ttttatctta aaccattttc ttccatcagt
    15121 ggatattttt ggtattttcc actaggcaaa aaacttgctc acataggagc gggggacaaa
    15181 aatagaaaac atgtggagga aactgactca tttttgaaaa aacatggcgg taaaattatc
    15241 aaaactgttg gaagaccaat acgtcttgca actcctgaca tgtgtgagcc tttctatcat
    15301 ggaaaggttg ttggagttgg agaatccatt ggtactgtat atccattgtt aggtgaagga
    15361 atcataccaa gcatgacttg cgcagatata tttgtagaaa atcttggaaa caatgaaaga
    15421 tatagacaac aagttcttga gaaatttgca atatatccta aagtgttgtc gtttgttagg
    15481 aacaagatgg actcaaagtt tggcatgcta aaacattttg ctgatctgat agcaatttac
    15541 agatacatga aaaagaatga aaaaagattt ggaatggaaa ttcgtatgac agacatgatg
    15601 aaggtcgcaa gggcttaacc tagagaaact gcaccaaaat tttctcgcac tgtcctgttt
    15661 gaactcatgt tatagtcgta tattatttta gaataatcta ggagaacatc tttcatttgg
    15721 tctatagatt ttgcaagata gaatcctgga cagtcaccag taaactgaaa tgtggcatga
    15781 actctcgctc gtcctctttc gttttcaagc caagaagaaa acggatacaa atagcagacc
    15841 cctggtctgc caggatgcaa accacagtaa cctttgtcat ctaaaaatcg acacgaaata
    15901 tgcgttccat cttcttttag aagttcatct ttttttcgtt ttagattaat cattgtcata
    15961 atcgtgttca tgtttccacc tggtccacct tcttcccacg tagcggtatt ggtctcattt
    16021 tttataaagt ccgaagtttt ttgatatttg aggcttttac caatggtgat gagatcgtct
    16081 gatgtaagtg gcagtcttcc ttgtctgtta caacaattat gacagtctgg ccaataacat
    16141 ttccataaga tgtagctgtc ctctttttgc aaatatggaa cgtgaaatac tacgtcttta
    16201 acttttatca tatattctga aacatcatct cggtttccca aaacgaattc tcgaatgatt
    16261 ggatcaatat cccagtcttt tgctaaaaga tcaagagaat cttcaatctc tttgcgcatt
    16321 aactaataaa ttacaactca acttttgaac gttatcaatc ttgagtgaca aaggaaaata
    16381 cgcttctgct actcaaaaca gaagactggt atgggaaaaa gtcatgtggc ctctaatttt
    16441 agagctaaat agcgtgagct ttaccatgga acagtataag aaaaagcgta atgagatctg
    16501 taaagagtta gagataccac catcaagaat tgccggaggt tttgtttcat tgttgctaaa
    16561 aggaatacta tacaaagaaa aagatctgta ttcaatccac tacagattaa ttccatatat
    16621 gcggctcaaa gctgattgcg attatgcaac agctgtacgc gaaacaacta ccaaataatt
    16681 atattctcag ctaatcaagt cgtgtcggta gcccgatagt ctagtggcca aacaattttt
    16741 gggttaggga ttccaggctc tggatcttac gagaggatac ctggggacgg cagttcgaat
    16801 ctgcctcggg ctactatttt ttgtaaaatc taatgtagaa ttatcgtgaa gccattgcta
    16861 ttctttctag ctcgtttttc ttttttacag aaggagcatt catatcattt gctgctgcca
    16921 aaatcagatg ctctgcaaga ttttcctcta ttgcttttgg atttgaaaac gacgcatctc
    16981 ttacgccttc tgcaatgaat cgtaatgcaa gatcaactct gcgcaatggt gctacatcta
    17041 cagagacatg ataaactgta ccaccataaa ctattcttgt cgtatcttcg tttggagctg
    17101 aattttcaat tgctctcacc aagacttcga tcgggttttt tccagtcttc aagtgcatta
    17161 tttcaaacgc tgctttaaca gtattaattg aacgagtctt ctttccaccc atccttccag
    17221 tgtttttcgc atatcttttt ccaaagtgta aaagcttgtt tgccagtctt tctactatgt
    17281 taacttcagc cttgttaaat cgctttagtg cagaacggcc aaaagtcatt ggcattatgg
    17341 tcttctttac agaaatggcg tttttcaatc ccggatcttt tatttcaata ttggagagat
    17401 cccattttct aaaaagtaaa agatttgcag tttctgccat ttctttatct ccttggcttc
    17461 tccttctttc catacaccaa ttctcttaat gaagtaccat ttactttgaa tactttccat
    17521 cttactccag gaatatctcc cattgcacct cccattgatg cacccattcc ctcaacatgc
    17581 acttcatcat gttcatcaac aaaattcatc gcaccgtctt ttggcaaaaa tgctgtaact
    17641 gtttttccat tttttataag ttgaactcta acacatttac gaacagcgga gtttggttgt
    17701 tttgcttcaa tgcctacttt ttccaaaaca attcctctag cttgcggagc accaccaagg
    17761 ggatttgctt ttttgtcaag acccaagttt cgtcttttgt atgttccaat tgcccatcgc
    17821 tgtttcttcc tcttatcttt aagaactcta cctgaaaata gaccgagggg ggattttgcc
    17881 atacattatc actccattat tttttccgga ctttgtatta aaacgctaga aatttgaaag
    17941 tatctttttg ctaaaagtct ggctttttca gcatttcgtc cttcacgacc taccactatg
    18001 cctttctttt ttgcatcaac catcactatt gcttgcattg agccgtcaag tcttttgttt
    18061 attttaactt cagaaaccag ctttgggttt agcatatttt tcaaaaagtc tgctggatct
    18121 tctgcgtatt caactaattc gacatttctt tttactacat tttgcaggtt tctgatggta
    18181 acgccacctt ttccaattgc aagccccatt ttacctggat ttacaacaaa tatgactctg
    18241 tcaagttttt catcttcaat gcaatctcgt gctgttgcgc cagttacatt ttggaataaa
    18301 gacataagtt tgatttggtc agttgtaagt ttgattgtct ctgtcatgtc tatgcctatt
    18361 ttaaaatcgg tgcctctcgg ggctcattta aagcttcatg aaaagttgca ttaaaataag
    18421 acatttattc ttggctctcc tgttccgctt ccttcaaaag tgactttaga tttgtttcac
    18481 tcaaagtttt tatcgaaagc gcggagattc taaattgcgt accacaaagt cttccaagtg
    18541 caacggatga gcccttaaaa tttaaggtag aggtttttga tgttttagcc gtttcctgaa
    18601 ttttttgtag aatattttgt ggaatagatt cagacaggat tactaatttt gaatttttca
    18661 tagagccaat tacttccttt gctccaagta aacatttgtt atcatccatt gcctctttga
    18721 ttattttttc caaaagttta gtcatcttgt actaccttca tgtatagatc cacggttcca
    18781 gtgcctatag gaatatttgc acctacaata acattttctg ttacaccttt gagatgttca
    18841 atttcaccag ctagtgcagc atcggcaatt gtaggaactg taatttcaaa tgcagctctt
    18901 gcaagcacac tagtttttgt tccagcgatt ccatgtctac caatttgttg caagtatcct
    18961 ttagaacaca tcaagtctgc aactagcatg atatatctaa cgtctacttc aagaccctga
    19021 tcttcaagtg tagagagtaa ttcatttatc aatgcatttc tagcagcttc aattccaagt
    19081 gtatttgcaa tttcaaagac attgtttgta cgaatgtttt tcttgtcaat tccctcaatt
    19141 tccaagactt tggcaatatt agaacctgaa gtctgtatga cccactcatc attttgttgc
    19201 actatggtaa ccctttctac atcggccaca ccttttacct tggttccaag aactttgttt
    19261 ctaagggcaa gtactgtttg tgcgtctgat tcttcagcaa gtgtaagcgt aatagagttt
    19321 ccactgggtt caatctcaaa tttctttttt gatgattgta atgcttcttc cacatcagca
    19381 acagtacatg ccctttctct cagtttatct gtgttaagat ttagttttat ttttgaagag
    19441 taatcagttt ctgtgctcga tataagagcg ctgatttttg tgtgcaagat ttcacgggca
    19501 acctgaattg ctttttcttt agattttttg aattttggct caagataaat gtccattgtt
    19561 ggtgttactg gttttttcct tgcatcaaca agttcaatca gtcttggcag acccaatgta
    19621 acgtttcttt ccctgattcc tgcaaagtgg aatgttctca aagtcatctg ggttccaggt
    19681 tcacctattg attgcgcagt tacaatacct acagcctgtc ccggctctac tttggccttt
    19741 tcatataatt ctaaagcttt cttggatacc ttttctacgc cttctttgct tagttttgat
    19801 tcaaataatg cttcttgaac tgtttgtgtt agccttggat taaatgtctt ggtatatttt
    19861 ttagcaagat cagcaatttc ttcttttgtt gcctttttac cagaatcaat tattgcttca
    19921 gattctacaa gtcttgcaat tctaaatgct tcgccatgat cactttttgc aacatcgatt
    19981 ccgtcctcac catacaagaa ttgtattatg tgaccatgcg gatctctgac tgttccatcg
    20041 tattctagtt taagatgttc taatgcattg atcaatcttc gctgcatgta accactttgt
    20101 tgtgttctaa ctgcagtatc caccaatcct tctcgtcctc ccattgcgtg gaagaaaaat
    20161 tccaaagtag aaagaccctc tctatagttt gattttacaa atcctcttgc atctggattt
    20221 gtatcatttt ctttgtaatg tggtaaggct cgattattgt atcctttttg tattctcttt
    20281 ccacggattg attgttgacc taaagctccc gccatttggc ctatgtttag agatgaacct
    20341 cttgcgccag ttgttgccat aattctacca gaatttgttt catcaaacga cttgtctgca
    20401 gtactacctg ccctgtctct tgcttttgac agttcattta cgatgtatgc ctctaatgct
    20461 tcatctgcag aaagacccct gcttagctgt aacgttccct tctttgcttg ggagatcaga
    20521 tcataaactt tgtcatatgt ttcttgtatt cccaccatga tgttttcttt cgttttttgt
    20581 gagagttcaa gatcagaata gccatagcta aagccatagt gtgtgataaa ctgttttagc
    20641 attatcagta tggaattaag aaactttttt gcctcctcat atccataatc ttttgtaatt
    20701 ctgtgcagaa cactatctgg ttcttctgcg ccgattgatg ctttgtctat tacaccgctg
    20761 attagttggc cattttttat tacaacgtct tttgcaggac cttttgttcc ctttgaccat
    20821 tttgatgtaa ttacataatt gaagtctttt ggcatgaaaa gcgagaaaag ttgtttgccg
    20881 gtgtacaatc ctccggtttt gtttttaact gcaggctctg gaaatgttcc atcatatcca
    20941 gcaatcattg caagatttgc aaattcttgt ggagtaagtg tagtaccatc ctttgttagc
    21001 agatatgccc cagtgataaa gtcacgcagt gcacctatga ttgggccacc atatcttgga
    21061 gagatcaatt ggtcttgaac acgcattaag agaattgctt ctgctcttgc ctcctcactt
    21121 tgtggaacat gcaaattcat ttcatctcca tcaaagtcag cgttgtatgg aggacataca
    21181 gaaggatgta atctgaatgt cttgtatggt agaactttaa cataatgagc catgattgac
    21241 atttggtgca gtgatggttg tctgttaaac atcacaatat caccatcgga aagatgtctc
    21301 tctatcagat atccaatctc aatgttgttt gctattgttt ctctatcttc tacaaaatcc
    21361 agacgaatct tgacaccatc tggtctaaca atgtaatttg cacctggaaa cttgtaaggt
    21421 ccattaatta caagcttctt tagtctttca atattccaat cagtaactac ttcaggtatc
    21481 gtgagtttga ttgcaacaga ttctggtact ccaacttcag aaatgtcaag atttggatct
    21541 ggagagatta cggttctgct ggaaaagtct actctttttc ccgacagcga acctctgaat
    21601 cttccttctt ttcccttcag tctttgtgtt agagttttta gtggtctacc tgatctgtga
    21661 tgagcttgtg gaattcctga aacttcgtta tcaaaatagg tagttgtgtg atactgtaac
    21721 agatcaacaa gatcctgcac aatcagaggt ggagttccag cttctttact ttcttttagt
    21781 ctttgattta ctcttataat gtctacaagt ttgtgcgtta gatcatcttc tgatctaatt
    21841 ccagtttcca aaataattga tggtctcacc gttacaggtg gaaccggtaa tacctgcaat
    21901 acaaaccatt ctggtcgtgc agtagtagga tcatatccta aaagtttcag atcatcgtcg
    21961 atcatgtgtg aaaatctttc tcgtattgta attggcagta atctattttc tcccagttct
    22021 gtcttttcaa tgaatattgt aggttttgta aaaataagat catattgaga ttttccacag
    22081 tgtggacagg ttttttcttt ttttgctttt tcaattattt catctggaat gtgttttatc
    22141 gagattacgg tatatgctgc ctctttgctt tttattttgt gatattgttc aagatcctct
    22201 tgtgaaattt tcagtcttgc acatgatctg catgtagtaa gtaaaagttt gtagatattg
    22261 tcaataaacg caatgtgtaa aacaggttca gcgagttcta tatgcccaaa gtgtccaggg
    22321 catcttgcag cggtgttgcc acatgtaaca catttttgtc caggttccaa agttcctagt
    22381 cgtccatcca tcaaacctcc ttgtacagac attccatctt cgtcataggt ttcaggtgca
    22441 gtgatttccg ctacagaata ttttctgatt tcagttggag accagacaga gaaacggatt
    22501 ccatcaatta cttttattgt ttcaagtgac attataccct ctccttaatc aaaagtcgtg
    22561 gggccacatc caagctcatc atttcttgca ataacagctt gaaggcatat gctactgaga
    22621 ttgaagttac tttggccttg tcaccacaga ctctgcaaac aaatcttctt tgctttatat
    22681 cataatatga aacgagtccg catctttcac agatgtagat gtctgccttg tctgattcat
    22741 caagtaatct gtcttttaac atcattgaag ctccatatgc gataaggcag tctctttcca
    22801 tttctccaaa tcttaaacca ccaccgcgtg cacgtccttc tgttggttgc tttgtaagca
    22861 tctggacttg tcctctagct ctagcatgaa tcttgtcagc aaccatgtga tgcagttttt
    22921 gataatacac tacgcctatg aagacttcag cttcgaatgc ttttcctgtt cttccatcat
    22981 acattatttc ttttccagaa tatttgaagc ctgcagattc tagaacacct tttacatcat
    23041 ccagtttttc tcctacaaat gcagatccat ccatttttgt tccacgcaaa gctgcagctt
    23101 ttcctgttac agattccaga aacattccta ctgtcattct agacgggaat gcgtgtggat
    23161 taatcagaac gtctggaaca actccatctg aagtatatgg taaatcttca tggttgacaa
    23221 gcatgccaac aactcctttc tgtccgtgtc gtgatgcaaa tttgtctcca atttcaggaa
    23281 ttctaacgtc tcttactctg attttataca tcttgccacc atcatgagat tgtgtcataa
    23341 ctacagtatc tacaactccg ttttctgatg gtcttacacc aattgatgta tctcttctgt
    23401 atggcccctt tacttcaaac tcacgatact cttccatgaa tcttggtgga ctagtttttc
    23461 caatcagaat atctccacct tgaacaggag attctgtcgc aattactcca tcttcttcta
    23521 ataatctgta agctttgtca cctctaaatc ctcgtatgtt tccttccgcg tttgggatct
    23581 caaagttgtc acgcattcca ccaggatatt gttttgcttc agcctcgtaa attctgtaga
    23641 agaaagttct tcccaatcct ctttctactg atgagcggct tagaacaatt gcatcttcaa
    23701 tattataacc atcaaaaggt aatactgcaa caacacagtt ttgtccagct ggtctatctt
    23761 ccaatcctaa aagtcccatt gcttttgttg taacaattgg agtttgtgga tacagcataa
    23821 agtgttgtct tacataggta cttgcgttca tcaacggagt ggagaatccg aggctttgtt
    23881 ttgccattgc agattcgtat gtatttcttg gagattggtt atgttctgga taaggtatga
    23941 ttgatgcacc tgcaccaaga atcgctgatg gaaatatttc catatgagta tgttttttca
    24001 ggttttcttc atccattgca atataacaat tttcttcctc gtttgcatca acaagctcaa
    24061 ttacgcccat gtggagaaga tcattccaag aaaggaattt tttactgact ttgtctatca
    24121 gttcttgtgt taagaggggt ttgttgtctt ttattacaat tagcggacgc aaaactcttc
    24181 cagcattaca gttaacatac agtctctttg tggatccctc aatgtttgat tgatacaaaa
    24241 atatgccaat atgcggatga attttgaagc ttcgtctaag atcacgtaaa gaatctgtta
    24301 gcttttgacc atccttataa tatccaatca gtctcccatc tacaaagact ctacttcctt
    24361 cttttttgag atcatctttt gcatctgaaa catatgttac accaagatca tagagctttt
    24421 ctatgatatc ttcggatgga acgtttactg atattattgc agaaagtgca agattcttta
    24481 ccaagccaca gtttgatcct tctggggttt cacttggaca aattctacca aagtgagttg
    24541 cgtgcaagtc tcttgcttca aagtttggtt gacttcggct aagtggtgat tgaattcttc
    24601 taagatggct aatcgtggaa agatagtttg ttctgtcaag cagttgtgtt actccaactc
    24661 tgcctcttcc ccagtttcca gttgcaattg cattgtttag tttgtctgtc actataccag
    24721 gtcttactgc agcagcaaca gcgttgattc ctcttttttg accagatctc tctagctggt
    24781 atttcatgtc acgtactagg tttctaaaag cagttcggaa tagatctgca agcatttgac
    24841 ctgcaaactt gatgactttg tttccatagt gatccttatc atctggagtt agccagccaa
    24901 gttttagttc aattaatttg catgcagctt cacccaaaaa ttgagctttt tcttttctgt
    24961 tgtcagggtt tttaccaaga tgtggtaata gaccccaatc aagtaaagtc tctgctcttt
    25021 ttatttgaaa ttcctctagc attccaggag ctattctctt actgatgtac acgattgcat
    25081 ctttagcagt tggaacatca ccagattttt caaatgatgc ttcaagctcg tcttgaatat
    25141 cgtttacaag tgaaactgcg gctgcaattt ctcgatcaga ttctagtcct agtgcacgca
    25201 tcaatgtaac tactggaata tctacaggag aaccaggaat ttttgcaaca attagtccat
    25261 cgtttttcat aacaagttcc agttttgcgc gataaccaac aattgaagaa tatactttag
    25321 ctttgaacac tgtatttcct ccaacggttt ctttgtcaac tataatttta ttataagaaa
    25381 gatcttccag tccaacaatt actctttctg agccgtttat gataaagtaa ccacctggat
    25441 catttggatc ttcaccatgc tccacaagtt tttgatcaga aaggttatgc aaaatacaag
    25501 cgtttgatct taccatgacg ggcatgtcac caatgtggac aaatcttgat tcaagaattt
    25561 ttccatcttc taccacactt gcttcaagca ttactggaga tgcatatgtt acatttctaa
    25621 gtcgtgcttc cattggagct atatgagtta ttgaaccatc aagctccatc attcttgctt
    25681 gttgaagttt taattttcca agttggattt tatatggata ttctgcactt tcaatttcga
    25741 tctgccctac ttcatcaatt atactttgca gaccacgttc cagaaattca tcgtatgaat
    25801 taagatgttg tctggcaata ccttctcttt ttagtatgtc ttgaattatg ggccaacgtt
    25861 tgtttgagat atttaccatt tattcttcca ccacgtatct gtaatagacg ctttcaccag
    25921 atgttgcact ttttcttgtt atttttatca tatcacctgg tttcacaccc aatcccttga
    25981 ttgcaggatc attttcgtaa ataagcggca tttctgtagg ctttgtgtga aattgttcca
    26041 atacttcttc ggcttccttt tttgtcatta tttcatgttt tggaacatag atgtgatccg
    26101 gtacaagtat gtgatctttt tttgttaaca atgtaaaaat ctccttaaaa caacattagc
    26161 tgtaaatgta caattaatcc acaaaccacg caaattgcgc cgagaggtta atatatatgt
    26221 aatctgggat ccgcactact aaattctgtt ttttgtcaac atttttttcc gtaaccttaa
    26281 atagttaaaa aataagttca aaaacaagat gagtactcgt tttaagggat tttccttact
    26341 tgcgattctg gttgcagcta ttggagctgt cccagctttt gcacaagtgg atccttttga
    26401 cacggtacca attactgtca cgacagacca agaatcatat gcagatggag acaccattgt
    26461 catctctggt gcaacaaaag actacatctc tgggcaacaa gttagtattg ttgttatcaa
    26521 tccaattggc aacatagtaa ctgtccaaca agtagatcta gaaaccgaca aaacatattc
    26581 tactgtactt actgcgggcg gaggcttgtg gaaagctgct ggcacataca cagtgaaagt
    26641 acaatatggc ggtgaaaaca gagcaaacga aacaacgttt gaattcagtg gatctgcagg
    26701 cggcccagga ggaaagaaga ttcatgtaga aggtactgac tttgatgtaa gatatagcat
    26761 cacaggcggt aaagtcttgg gaatcacagc tgacgtagaa gcaaaatctt tgcttgtttc
    26821 catagaaaca acaagtgatg gagtattgac cataactcta ccaagagctt tgatcgatgc
    26881 aaagatcaat ggcgaagatg atagtttctt cgttctaata gatggtgaag aaataggcga
    26941 tgtcagtgaa gaaacaacat ctacggacag gactttgaca attccattca cagatggttc
    27001 tgaagagata gagatcattg gtacacaaat agttccagag tttggtgcag tagcagcttt
    27061 gatacttgca gttgcaatta tatcaataat tgcggtttct gcaaagacca ggctaagact
    27121 aatgccaaaa tactagaact caaatctttc attttttaag attctatta
//
LOCUS       DATIDL010000063         8149 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrosopumilaceae archaeon isolate SMAG_U16342
            k141_1085418, whole genome shotgun sequence.
ACCESSION   DATIDL010000063 DATIDL010000000
VERSION     DATIDL010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935836
            Sequence Read Archive: SRR25158326
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosopumilaceae archaeon (soil metagenome)
  ORGANISM  Nitrosopumilaceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrosopumilales;
            Nitrosopumilaceae.
REFERENCE   1  (bases 1 to 8149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/04/2024 05:09:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,520
            CDSs (total)                 :: 1,474
            Genes (coding)               :: 1,474
            CDSs (with protein)          :: 1,474
            Genes (RNA)                  :: 46
            rRNAs                        :: 1 (5S)
            complete rRNAs               :: 1 (5S)
            tRNAs                        :: 43
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8149
                     /organism="Nitrosopumilaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1085418"
                     /isolate="SMAG_U16342"
                     /isolation_source="soil"
                     /db_xref="taxon:2202732"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..539)
                     /locus_tag="VLB45_02850"
     CDS             complement(<1..539)
                     /locus_tag="VLB45_02850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase"
                     /protein_id="HSD04672.1"
                     /translation="MKKKTKAILVSIFFGLILVTSTITLALIGNVPSNNDDGTVTKTG
                     TPADNYKDAERETFCGTGSVKSNRYITEFKIPTVCTQPLAITTDSSGNVWFTESNTGN
                     LAKFDPFSRTFTEFENTKWPEGDQSMMWGIDHSQDENIWFTDERHDVIWKFSILEKNF
                     TSYKYPASDSEATFPQKLIV"
     gene            complement(575..931)
                     /gene="pth2"
                     /locus_tag="VLB45_02855"
     CDS             complement(575..931)
                     /gene="pth2"
                     /locus_tag="VLB45_02855"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010191192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-tRNA hydrolase Pth2"
                     /protein_id="HSD04673.1"
                     /translation="MGAIKQVIVVRKDLDMGTGKIAAQVGHACVLGAEHVRKSYPEWY
                     KEWIMLGQEKVVVKVTSLEQLEQVKRHAITLDLPWSEVTDAGHTQIAPGTVTCISIGP
                     APETTIDKVTKDLKLL"
     gene            1003..2139
                     /locus_tag="VLB45_02860"
     CDS             1003..2139
                     /locus_tag="VLB45_02860"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007551057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HSD04674.1"
                     /translation="MDYDVIVAGGSVSGLLCAREIARLGHSVLVIEEDYEIGTPEHCG
                     GLVSISGIEELGVVPTRNTLDNKIRSASIISPGGKTFSLDARSQKVVVLDRRDFDKQI
                     AHGAKNNGAEIRVKTSFKEFKDGEVKTSDGTMKCKMLVDARGVSSLIQKDREGILQSA
                     QYEVYADWIDKERIEVYFDQEKYPGFFAWVIPSHHDIAKVGVAGKGINAAMALENFLR
                     QKGKHSVVRKVFAPIWIKGPIENFVTKDTIIIGDAAGQSKPTTAGGIYSCGLGGIFAG
                     KAISKFLESKNHSDLLEYQKAWLEKFGQEFEHMLLARKLLERLDNKSIDKIFESVTPQ
                     ILEEISKEGTFDFHTVSLAKLLGVKGSVKAVQAILGSEFRRLLT"
     gene            2195..2374
                     /locus_tag="VLB45_02865"
     CDS             2195..2374
                     /locus_tag="VLB45_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc finger domain-containing protein"
                     /protein_id="HSD04675.1"
                     /translation="MSSSIALPVCSSCHRTIMPNDKCVKFNCPNCGSILLWRCESCRE
                     AARSYKCNSCNFNGP"
     gene            2378..2659
                     /locus_tag="VLB45_02870"
     CDS             2378..2659
                     /locus_tag="VLB45_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 1-beta"
                     /protein_id="HSD04676.1"
                     /translation="MARLLLKAKILPTGTEIELDGIAKNVSAALKDGIQLVKYTKEPL
                     AFGLYFINAEFSLDDKEGQMDSLENAVRSIEGVGEFEVLGMSRASVDVK"
     gene            2668..4839
                     /locus_tag="VLB45_02875"
     CDS             2668..4839
                     /locus_tag="VLB45_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDC48 family AAA ATPase"
                     /protein_id="HSD04677.1"
                     /translation="MVRKDEPLQMRVGEAKQRDVGKRRARIEPEAMEYLRVEPGDMVE
                     IVGKRSSCAIVWPTDDDDKDPDMIRIDGQTRKNVGVSVNDLVSVRKIVAKPAKSVVLM
                     PVNGNVTVDKEFTDFVKNRLKGLPLSEGDEISVMILGNSMDFKIHKISPKTVVKIERI
                     TTVTILTETSVDKKVRVTYEEIGGLKGQTKRMREIVELPLRHPEVFSRLGIEPHSGIL
                     LYGPPGCGKTLIAKVLASESEANFYSINGPEIMNKYYGETEARLRDIFKEAKENAPAV
                     IFIDEIDAIAPKREEAYGDVEKRVVAQLLALMDGLTDRGNVIVLGATNRPESVDPALR
                     RPGRFDREIEISVPNADGRLEILHIHTRGMPLDTDVDLKMLAAELHGYTGADIKSLCR
                     EAAMKSLRNYLPEIDLETEKISSEVLQSMRIRLQDFYDAMHEIVPTAMREFYVESPKV
                     WWKDVGGQDDAKRTLHDNLIMAIKEPGKFQKMGIKPPKGALLYGPPGCGKTLLARALA
                     TESGANMILVRGPEVLSKWVGESEKAIREIFRKAKTSSPCIVIFDELDSFARSKSGED
                     GGVGERLLSQLLTEMEDSGASRVIVIGISNRPDLIETSLLRPGRLDLILYVQPPDEKG
                     RLEIIKILTRTMPLSNDVDLKEIAVATKSYSGADLVAICREAAVHAMQNDSVKIGSAD
                     FAAALKLVKPSITKSVDEWYGTIKENISYAIPKPIDKTFYG"
     gene            4850..5158
                     /locus_tag="VLB45_02880"
     CDS             4850..5158
                     /locus_tag="VLB45_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007551060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04678.1"
                     /translation="MPLKNFVYEKIKDSGSLTASELSKILVKTGMVIPEDEFEKILLS
                     LEIYGLIKVSWLTKDERRIEIIEKESEKDEIEEQNREKLERDYEAGFPGIEQEQDQEV
                     "
     gene            complement(5159..6031)
                     /gene="cyoE"
                     /locus_tag="VLB45_02885"
     CDS             complement(5159..6031)
                     /gene="cyoE"
                     /locus_tag="VLB45_02885"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HSD04679.1"
                     /translation="MGLKEILEISKPRIVILLVITAVTSMYAASKLIGPELNYWSLLH
                     IIISGALASAGSSALNHYYDKDIDPLMKRTSTRPIPSGRMSAKNVLWYGLAVSAISVI
                     YAGITLNLVSTFFIALGIFFYVIVYTAWLKRNNASNIVIGGFAGSAASMAGWSAATGS
                     MDLLGTLVGMLVFVWTPSHFWCLAMKIRDDYAEAKVPMLPVLVGMQKTSKYILVNTAI
                     LLPYSLALVAFGLGYVYLVIAAISGGLMLAYHYKLTKNPTSEFAWKAYKVTAPYLTII
                     FVAIALDAAFHFGI"
     gene            6129..7238
                     /locus_tag="VLB45_02890"
     CDS             6129..7238
                     /locus_tag="VLB45_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016939293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HSD04680.1"
                     /translation="MSDWDLMMPGMGLTAIGLAGVVVSYAGIAKTFIDGMHALTGLTM
                     FVGLIFLACGILNGGVSTSNRAKATTLVIFGIAGSFGAFAFALTSVTTLSTFAGVLLI
                     IIIPTIVISYAAMKVPQYAKPISVIFILAAGVGIASFVTLGFVGPGAQFSLQPPVMEE
                     AEKPMEEPIPVTANIVSVEILAGASIPDSPDYSPEPVTVTKGDVIEWTNNDNVMHTVT
                     SLADAGTTFDSSVISAGTKWDLDTKDLAAGEYEYFCTVHPFMTSKIIISEPAEPVVAK
                     VSIPSGAMTQSEGQLYYDPQDIVISSGTTVVWSNDDTAAHTVTSGNPTDGASGMFDSS
                     ILPAGQTFEHKFDIPGKTEYYCQVHPWMVGTVTVE"
     gene            7241..7648
                     /locus_tag="VLB45_02895"
     CDS             7241..7648
                     /locus_tag="VLB45_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M67 family metallopeptidase"
                     /protein_id="HSD04681.1"
                     /translation="MQKGIVLSQHQIDILVKHARDNSPNESCAILFGKVENDHFAVKD
                     IFLTKNTENSPVNFTISNDELIKGYDEAEKRNLDVVGIFHSHPNSEAHPSPTDKKYME
                     INPVPWVIFSNQSEQFRAYVFESTIKSLDLIVQ"
     gene            complement(7791..8141)
                     /locus_tag="VLB45_02900"
     CDS             complement(7791..8141)
                     /locus_tag="VLB45_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclophilin-like family protein"
                     /protein_id="HSD04682.1"
                     /translation="MDLELDDRFSPKTLSAFLDCLPLTVGINSWGEELYTDETPVRVA
                     EENAKPLVDLMDVAYWPTGKAICLFFGPTPIGTKDEIKPYSPVNVIGKIINADKRFLK
                     DVPDGMVVTFRLSK"
BASE COUNT         2675 a         1468 c         1686 g         2320 t
ORIGIN      
        1 actatgagtt tttgtggaaa agttgcttcc gaatcagaag ctggatattt gtaacttgta
       61 aaatttttct ctaaaataga aaacttccag attacatcat gacgttcatc tgtaaaccag
      121 atgttttcat cttgggaatg atctattccc cacatcatgg attggtcacc ttctggccac
      181 tttgtattct caaattctgt gaaggttctc gaaaagggat caaactttgc aagatttcct
      241 gtgtttgatt ctgtgaacca cacatttcca gatgaatcag ttgttatggc aagtggctgt
      301 gtgcacacag ttggaatttt aaactcagtt atgtatctat tagattttac actgccagta
      361 ccgcaaaaag tttctctctc ggcatccttg taattgtcag caggcgttcc agtttttgta
      421 actgtaccat cgtcattgtt tgatggaaca ttgccaataa gggctagggt tatagttgat
      481 gttactaata tcaacccaaa aaatatagaa actaaaattg cctttgtttt tttcttcaca
      541 agaggcagac aagttttcct tgttatatct tattctacaa aagtttcaag tcttttgtga
      601 ctttgtcaat agttgtttct ggtgcaggtc cgatcgaaat gcaagttacc gttccaggtg
      661 caatctgtgt atgtcctgca tcagttactt cagaccatgg aagatctaat gttatggcgt
      721 gtctttttac ttgttctagt tgttcaagac tagtaacttt gacaactact ttttcctgac
      781 ctagcataat ccattctttg taccattctg gatatgattt tctaacatgt tctgcgccta
      841 agacacatgc atgtccaact tgggcagcta tttttcccgt acccatatct agatcctttc
      901 taaccacgat tacttgcttg attgcaccca tactttcaca aagaaattag tctatgttaa
      961 acttttgaat tgaaataaat aattgcaaca aaatcaaaca ttgtggatta cgacgtcata
     1021 gttgcaggtg gaagtgtttc cggattatta tgtgcaaggg aaatagctag acttggtcat
     1081 agtgttttag ttatagaaga agactatgag attggtaccc cagaacactg tggtggtctt
     1141 gtcagcatct caggaataga agagctggga gtagttccaa caaggaatac tcttgacaac
     1201 aaaatacgct ctgcatcaat aatctcccct ggaggaaaga ccttctcact tgacgcaaga
     1261 tctcaaaaag ttgtagtctt ggatagaagg gattttgata aacaaattgc acatggcgca
     1321 aaaaataacg gcgcagaaat tcgtgtcaaa acttcattta aagaattcaa agatggcgag
     1381 gtaaaaacat ctgacggtac aatgaaatgc aaaatgttgg tagatgccag aggtgtgtca
     1441 tcacttattc aaaaagatag ggagggaata ttgcagtctg cgcagtatga agtatatgct
     1501 gattggattg acaaagaaag aattgaagta tattttgatc aggaaaaata tccgggtttt
     1561 tttgcatggg ttatcccttc acaccatgac attgccaaag ttggcgttgc aggaaagggc
     1621 atcaatgcag caatggcact agaaaacttt cttagacaaa aaggaaaaca ctctgttgta
     1681 cgaaaggtct ttgccccaat ctggataaag ggtccaattg aaaattttgt caccaaagac
     1741 accattatca taggtgatgc cgcaggccag tcaaaaccca ccactgccgg aggcatatat
     1801 tcttgcggtc tgggtggcat atttgcaggc aaagcgatct caaaatttct ggaatcaaaa
     1861 aatcactctg atctattaga atatcagaaa gcatggttgg aaaaatttgg tcaggagttt
     1921 gaacacatgc ttctcgctag aaaactactt gaaagactag ataacaaatc tattgacaag
     1981 atctttgaat ccgtgacgcc acaaattcta gaagagatct ccaaagaagg aacttttgac
     2041 tttcatactg tttctcttgc caaactcttg ggagtaaagg gatctgtcaa ggcagtccaa
     2101 gcaattttgg gaagcgagtt tagaagactg ttgacgtgag ataacataca attgataagc
     2161 aagatataaa ttcggttttt aatcagctca gaacatgtcg tcatctattg cattgccagt
     2221 gtgtagttca tgccacagaa ccataatgcc taatgataaa tgtgtaaaat tcaactgccc
     2281 aaactgtgga tctatactac tttggagatg tgaaagctgt agagaagctg caagatctta
     2341 caaatgtaac tcatgcaact ttaatggacc ataaaaaatg gcacgccttt tacttaaagc
     2401 aaaaatcctt ccaacaggca cagaaataga actagatggt atagcaaaaa atgtaagcgc
     2461 tgcactaaag gatggaattc agcttgtaaa atatacaaaa gagccacttg cttttggtct
     2521 atactttatt aacgcagaat tttccctaga tgacaaggaa ggacagatgg actcgcttga
     2581 aaatgcagtc agatccattg aaggtgttgg agaattcgaa gtcttaggaa tgagcagagc
     2641 atctgttgat gtaaagtagg tgtttttttg gtacgcaaag acgaaccatt gcaaatgcgt
     2701 gttggtgaag caaaacaacg cgacgtagga aaaagacgtg caaggataga accagaagcc
     2761 atggaatacc ttagagtaga acctggtgac atggtggaaa tagttggaaa aagatctagc
     2821 tgtgctatag tctggccaac agatgatgat gacaaggatc cagacatgat acgaattgac
     2881 ggtcaaacaa gaaagaatgt tggtgtttca gtaaatgatc ttgttagtgt aagaaaaatt
     2941 gtagcaaaac cagcaaaaag cgtagttttg atgccggtaa atggcaatgt cacggtagac
     3001 aaggaattta cagattttgt caaaaataga ctgaagggat tgcctctctc agaaggagat
     3061 gagatatctg taatgattct tggaaattcg atggacttta aaattcataa aatttctcca
     3121 aaaactgttg tcaagataga gcgaataacg acagttacta ttctaactga aacatcagtt
     3181 gacaaaaaag ttcgcgttac ctatgaagag attggtggtc taaaaggaca aacaaaaaga
     3241 atgcgagaaa tagtggaact gccacttcga catcccgaag tctttagcag attaggaatt
     3301 gaaccacata gtggtatctt attgtatggt cctccgggat gtggaaaaac tttgattgcc
     3361 aaggttcttg caagtgaatc agaagcaaac ttttactcta ttaacggtcc tgaaattatg
     3421 aacaagtatt acggagaaac tgaagcaaga ctgcgtgata tattcaaaga agcaaaagaa
     3481 aatgcaccag ctgtaatatt cattgatgaa attgatgcaa ttgctccaaa aagagaagaa
     3541 gcatatggtg acgttgaaaa aagagtagta gctcagctct tagctttgat ggatggtctg
     3601 actgacagag gaaatgttat tgttttaggt gcaacaaaca gaccagaaag tgtagatcct
     3661 gctcttagaa gacccggcag gtttgataga gaaattgaga tttctgtacc aaacgcagat
     3721 ggtagactgg aaatcttaca tatccatact agaggaatgc cacttgatac agatgtagat
     3781 cttaaaatgc tagctgcaga acttcatgga tatactggtg cagacataaa atcactatgt
     3841 agagaagctg ccatgaaatc gcttcgaaac tatttgccag agattgattt ggaaacagaa
     3901 aagatttcat ctgaagtttt acaatcaatg agaattcgtt tacaggactt ttacgatgca
     3961 atgcatgaaa ttgtacctac tgcaatgaga gaattctatg ttgaaagtcc caaagtgtgg
     4021 tggaaagatg ttggcggaca agatgatgca aaaagaacac tacatgataa cttgattatg
     4081 gcaataaaag aaccaggaaa attccaaaag atggggatca aacctccaaa aggtgcactg
     4141 ttgtatggtc cacctggctg tggaaagacg ttgcttgcaa gagcacttgc aacagaaagt
     4201 ggagccaaca tgattcttgt acgcggtcct gaagttctat ctaaatgggt tggcgaatca
     4261 gaaaaggcaa ttcgagaaat tttcagaaag gcaaagactt catccccatg tattgtgata
     4321 tttgatgagc ttgattcttt tgccagatca aaatctggtg aagatggtgg agtaggcgag
     4381 agacttttga gtcaactctt gacagaaatg gaagatagcg gagcctcaag agttatcgta
     4441 attggaatat caaacagacc tgatctaata gaaacttcgc ttttacgacc aggacgacta
     4501 gatttaattc tgtatgtaca accaccagat gaaaaaggaa gactggaaat aatcaagatt
     4561 cttacacgta ccatgcctct ttctaatgat gtagatttga aggagattgc agttgctacg
     4621 aaaagctata gtggagctga tcttgtagct atttgtagag aagcagcggt acacgcaatg
     4681 caaaacgatt ctgtaaaaat aggaagcgca gattttgctg ctgccctaaa actagtcaaa
     4741 ccatccataa ctaagagtgt ggatgaatgg tatggtacaa taaaagagaa tatatcatac
     4801 gccataccaa aaccaatcga taagacattt tacggttaga tatatgacaa tgccattgaa
     4861 aaatttcgta tatgaaaaga taaaggattc tgggtccctc acagcttctg aactgtcaaa
     4921 gattcttgta aagacaggaa tggttattcc agaagatgag tttgaaaaaa ttctactaag
     4981 tctagagatc tatggattga tcaaagtaag ctggctcaca aaagacgaac gccgaataga
     5041 gataattgaa aaggagagcg aaaaagacga aattgaagaa caaaatagag agaagctaga
     5101 acgagattat gaagctggat ttccaggtat tgaacaagaa caagatcaag aagtataatt
     5161 agattccaaa gtgaaacgct gcatcaagtg ctattgctac aaatattatt gtaaggtatg
     5221 gtgcagtaac tttgtatgcc ttccacgcaa attcagaagt tgggttcttt gtaagtttgt
     5281 aatgatatgc taacataaga ccgccagaaa ttgctgcaat tacaagatac acatatccta
     5341 atccaaatgc aactagtgct aaagaatatg gaagtaggat tgcagtatta accaaaatgt
     5401 actttgatgt cttttgcata cctactaaaa caggcagcat gggaactttg gcttctgcat
     5461 aatcatcgcg aatcttcatg gcaagacacc aaaaatgaga tggagtccag acaaaaacta
     5521 acattcctac caatgttcca agtaaatcca ttgaaccagt tgcagctgac caccctgcca
     5581 tagaggcggc acttcctgcg aatccgccaa ttacaatatt tgatgcatta tttcgtttaa
     5641 gccacgcagt gtatactatg acataaaaga aaattcctaa tgcaatgaaa aaggtagaaa
     5701 caagatttaa cgtaattcca gcataaatta ctgaaatggc actaactgct aaaccatacc
     5761 aaaggacatt ttttgcagac attctgccag atggaatcgg tcttgtacta gttcgtttca
     5821 tgagcggatc gatgtctttg tcgtagtagt gatttagtgc actagaacct gcagaagcaa
     5881 gtgcaccaga tatgatgatg tgcaaaagac tccaataatt tagttctgga ccgatcagct
     5941 tacttgctgc atacatggat gtgactgcag taatgacaag cagaataacg atacgcggtt
     6001 ttgatatttc taagatctct tttagtccca atctctttgt agcagttttg ttggggattt
     6061 aatttcttcg aagctgtttg ccttgggtgc aaaaggatta tttatctcac ttcttgcatc
     6121 taccacaaat gagcgactgg gatcttatga tgcctggaat gggtcttact gctataggtc
     6181 tggcaggcgt agtcgtttca tacgctggca tcgcaaaaac cttcatcgat ggcatgcatg
     6241 ctcttacagg acttacaatg tttgttggtc tgatatttct ggcctgtgga attcttaatg
     6301 gtggcgtttc aacaagcaac cgagctaaag ccaccactct agtaatattt ggaattgcag
     6361 gatcatttgg agcatttgca ttcgcactga cttcggtcac aacactttcc acatttgcag
     6421 gtgttttact aatcataatc atcccaacaa tagtgatttc ttatgcggca atgaaagtgc
     6481 cacaatatgc caagccgata tctgtgatat ttatactggc agcaggtgtt ggcattgcat
     6541 catttgttac cttgggattt gtaggtcctg gagcacaatt cagtttacaa ccgccagtaa
     6601 tggaagaagc agaaaaaccc atggaagagc caattcctgt tactgcaaac atagtatcag
     6661 ttgagatact tgccggtgct tcaattccag acagtcctga ttatagtcca gaacctgtta
     6721 ctgtaacaaa aggcgatgtg attgaatgga caaataatga taatgtcatg catacagtaa
     6781 caagtctagc tgatgctggt acaacatttg attccagcgt aatatctgca ggaaccaaat
     6841 gggatcttga cacaaaagat cttgctgcag gtgaatacga atatttctgt actgtgcatc
     6901 catttatgac ctcgaaaatt attatttcag aacctgctga accagtagtt gccaaagtaa
     6961 gcataccttc aggagcaatg acacaaagtg aaggacaatt atattatgat ccgcaagaca
     7021 ttgtcatttc ttcaggaact acagtggttt ggtccaatga tgacaccgca gctcatactg
     7081 tcacaagcgg aaatccaact gatggtgcat ctggcatgtt tgattctagc atattgccag
     7141 caggtcaaac attcgaacac aagttcgata ttcctggaaa aacagaatac tactgtcaag
     7201 ttcatccctg gatggtagga acggtaacag tagaatagct ttgcaaaaag gaatcgtact
     7261 ttcacaacat caaatagata ttcttgttaa acatgcaaga gataattctc ctaacgaatc
     7321 atgtgcgatt ttgtttggca aggtagaaaa tgatcacttt gcggtaaaag atattttttt
     7381 aacaaaaaac actgagaact caccagtaaa tttcacaata tcaaatgatg aactcataaa
     7441 aggatatgat gaagccgaaa aaagaaatct tgatgttgta ggaatttttc actctcatcc
     7501 aaactcagaa gcacatccat cacctacaga caaaaaatat atggaaataa accctgtacc
     7561 gtgggtaata ttttcaaacc agtctgaaca gtttagagca tatgtatttg aatcaaccat
     7621 taaatctcta gatctaattg tgcagtaatt atttgtggat gtttttcttg tgcctttcta
     7681 gatgttcctt gtcagtaaag gatacgttac actccttgca tttgagcgga gttttaaagt
     7741 tccacaagtg tatgtcatac atcatcatag tgtaaaagca ttctgatcaa ttatttgctt
     7801 agcctaaatg ttacaaccat gccatcagga acatccttga gaaaccgctt gtcagcgttt
     7861 attattttgc ctatcacatt tactggagag tatggcttta tctcatcttt tgttccaatt
     7921 ggtgttggtc caaaaaagag acagattgct tttcctgtag gccagtatgc gacatccatc
     7981 aaatcaacaa gtggttttgc attttcttca gccactctga ctggggtttc atctgtgtaa
     8041 agctcttcgc cccatgagtt tattcctaca gttagaggca agcaatcaag aaaagcactg
     8101 agcgtctttg gagaaaaccg atcgtctaat tctaaatcta tagtcttta
//
LOCUS       DATIDL010000064         5689 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrosopumilaceae archaeon isolate SMAG_U16342
            k141_885181, whole genome shotgun sequence.
ACCESSION   DATIDL010000064 DATIDL010000000
VERSION     DATIDL010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935836
            Sequence Read Archive: SRR25158326
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosopumilaceae archaeon (soil metagenome)
  ORGANISM  Nitrosopumilaceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrosopumilales;
            Nitrosopumilaceae.
REFERENCE   1  (bases 1 to 5689)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5689)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/04/2024 05:09:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,520
            CDSs (total)                 :: 1,474
            Genes (coding)               :: 1,474
            CDSs (with protein)          :: 1,474
            Genes (RNA)                  :: 46
            rRNAs                        :: 1 (5S)
            complete rRNAs               :: 1 (5S)
            tRNAs                        :: 43
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5689
                     /organism="Nitrosopumilaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_885181"
                     /isolate="SMAG_U16342"
                     /isolation_source="soil"
                     /db_xref="taxon:2202732"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1244
                     /locus_tag="VLB45_02905"
     CDS             <1..1244
                     /locus_tag="VLB45_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042242 - cobyrinic acid a,c-diamide
                     synthase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cobyrinate a,c-diamide synthase"
                     /protein_id="HSD04683.1"
                     /translation="KVGPDFIDPSYLSAVSGRPARNLDPWIMGLQGVMKSFTKNSDSD
                     FSVTEGVMGYYDGFSGNSNFSSTYHVAKIIQAPTILVLDASKTARSIAATALGFTKFQ
                     KNSRIIGFILNKVGSKKHEDLCKQALAPLKLPILGVIPRNPDLTMESRHLGLIPVREQ
                     QSLRSKIKKIARTMSDFVDIDTIIQIGKKVSPLPQTLPENKVKQKTTIAVALDESFNF
                     YYQDNLDALRISGAKIEFFSPVSDSKVPQCDGIYIGGGFPEVIGSSLEKNQKMQKNIK
                     KMAESGIPIYAECGGLMYLTKSIDYGSKKFKMIGLFDATTKMQKKLKLNYTKATVSHN
                     CIIANTENIIQGHEFHFSELESIRNDSKFAYSLSVGIGIKDKKDGLMEYNTLASYMHV
                     HFARSNTAKKFVQNCIKNSRR"
     gene            complement(1233..1871)
                     /locus_tag="VLB45_02910"
     CDS             complement(1233..1871)
                     /locus_tag="VLB45_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008299909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-8X methylmutase"
                     /protein_id="HSD04684.1"
                     /translation="MITNKGQSIEDKSMEIIESEIGSHSYNKDEWPIVRRIIHATADF
                     DFARQNMMIFHKNAIQNGILALRNGCNIIVDVNGVIGGFNKENLKEFGNKAICKISEL
                     EIANEAKKLNKTRAQISMRMSALEMNGGIVAIGNAPTALVEVINMIKEGITKPALVIG
                     IPVGFVCATESKEELQTLEIPYITNKGRKGGSPCASAIVNALFKLLKEKSSS"
     gene            complement(1868..2614)
                     /locus_tag="VLB45_02915"
     CDS             complement(1868..2614)
                     /locus_tag="VLB45_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CbiX/SirB N-terminal domain-containing protein"
                     /protein_id="HSD04685.1"
                     /translation="MKRGLLLIDRGSREKEVQDELRYICEQVKTKGNYLFCNYCFLEV
                     IPPFIEEGIEKCLKHEIDALTIVPYFLYPGKKVKAAVNAAIAMQSKTKVKLVISKPMT
                     LHKSMVELVDKKIRSTLVKNSISLPKKEIDVLIICHGSKDPNAQMSIKYVVDDLSGSY
                     RNVDYCFLEIEEPNIAQGIEKCVRNKPKALVVMFYFLHEGAHVKRDIYTDLNPAIEKS
                     KLSNIFVTEHLGTDDKMIDLVIERAKEVEK"
     gene            complement(2611..3675)
                     /locus_tag="VLB45_02920"
     CDS             complement(2611..3675)
                     /locus_tag="VLB45_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HSD04686.1"
                     /translation="MEKVAVLAITKNGINIGLSLKNQFSGWKIYAPSKFSSNNPEVEW
                     FDDSTTVKIGELFKTNDALVCVFSLGAVIRLIAPHMKDKKTDPAVIVIDDTGKFVIST
                     LSGHLGGANQLAEEIARVLNATAIITTAADVNKTIAVDLVGKEFGWKIDDDSIVTKVS
                     AFMVNEEKIGVFQDSGEKEWWLPKKELPKNVTIYNSLEELESSDCKGFLIISDRIIEK
                     KELLKNAVVYRPKTLVIGVGLHWDTTRDTIMEGLNHCMKKFNISPKSIARFASIKKET
                     NVKGLQDLANELNVPVDYYEKDELAQITIPNPSETVQAFEGTPSVSEASALRSSGGKL
                     IVEKQKFPPNLTIAIARILK"
     gene            complement(3708..4805)
                     /locus_tag="VLB45_02925"
     CDS             complement(3708..4805)
                     /locus_tag="VLB45_02925"
                     /EC_number="2.1.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-5B (C(1))-methyltransferase"
                     /protein_id="HSD04687.1"
                     /translation="MENEIASKGKLRTGFTTGTCATAGAKAGILSIMNQAKIDSVKVI
                     LPKKKFIDVKISNCQFNSKSAKCMVIKDGGDDPDVTHGAEIWTEVFLTNKPNQIEING
                     GEGVGRVTKPGLGLDVGSAAINPTPKKMISENILDVAKDILKKNGINIVISVPKGKEL
                     APKTDNPRLGILGGISILGTSGIVIPYSTASFAASIRQSLDVTIAMGNDTVVLTTGGR
                     SEDFARTIINLPDHCFVQMGDFSGYAVQQCAKKKIKKAYVAGFIGKLTKMAMGVKQTH
                     VKGSKVDMEFLSKLAQQCNADVKIVHEIKKANTARHVFEIIVREKLEGFFDRVCFEVY
                     KQLRNHSENEFEIDVIMFDFEGKIIGKHPKQ"
     gene            complement(4845..5291)
                     /locus_tag="VLB45_02930"
     CDS             complement(4845..5291)
                     /locus_tag="VLB45_02930"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HSD04688.1"
                     /translation="MNSSKCYEILGIQKGASQKEIKTAYRNLSLKYHPDRNKDEKDGE
                     KFKAVIEAYQFLRREEKQSQKKSDADIESTYTDFWKYYDKATNEEYQFSAKTNFDKFW
                     QSFGVNVNEAYELNQEKPTSHITTHVLLYGGLGAIALWIILSGILK"
     gene            5343..>5689
                     /locus_tag="VLB45_02935"
     CDS             5343..>5689
                     /locus_tag="VLB45_02935"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HSD04689.1"
                     /translation="MHNKSLTIAITGANGFVGRNIGKIFAKKGIKIIGIIRKNKKSSI
                     TFGIPVISDDLSEKYLISRLKGCSALLHLIGQGRQTVDSDYEKVNVGLTKNAIRLCKK
                     AGIKKIIYISGFGV"
BASE COUNT         1695 a         1081 c          863 g         2050 t
ORIGIN      
        1 ttaaggttgg gcctgatttt atagatccca gctatctttc tgcagtttct ggcagacctg
       61 cacgaaatct agatccgtgg attatgggtt tacaaggcgt catgaaaagt tttacaaaaa
      121 attctgattc tgatttttct gtaactgaag gagttatggg atactatgat ggtttttctg
      181 gaaattcaaa cttttcaagt acataccatg ttgctaaaat tatacaagca ccaactattc
      241 ttgtcctaga tgcaagtaag acagcacgct ccatagctgc tactgcactt ggatttacaa
      301 aatttcagaa aaattctcga ataattgggt ttatcttaaa taaagtagga agcaaaaaac
      361 acgaggatct ttgtaaacaa gcccttgccc cattaaaact accaatactt ggtgtaattc
      421 caagaaaccc tgatcttact atggagtcaa gacatttagg attgattcca gtcagagagc
      481 agcaatcgct ccgatctaaa ataaaaaaaa ttgcaagaac aatgtccgat tttgttgata
      541 ttgatactat aattcaaatc ggaaaaaaag tttcacctct tccacaaact ttgccagaaa
      601 ataaagtgaa acaaaaaacc acaattgcag tagcactgga tgaatcattc aatttttact
      661 atcaagataa tcttgatgcg cttagaataa gcggtgcaaa gattgaattt tttagtcctg
      721 tttctgattc caaagtgccg cagtgcgacg gtatctacat cggaggtggt tttccagaag
      781 tcattggcag ctctcttgaa aaaaatcaaa aaatgcaaaa aaatattaaa aaaatggctg
      841 aatctggtat tcctatatat gctgaatgcg gtggattgat gtatcttaca aaatcaatag
      901 attatggatc aaaaaaattc aaaatgatag gactttttga tgctacaacc aagatgcaaa
      961 aaaagctcaa actgaattat acaaaggcta cagtttctca caactgtatt attgcaaata
     1021 ctgaaaatat tatacaggga cacgaatttc atttttctga attagaatcc atacgtaatg
     1081 attccaagtt tgcatattca ctctctgttg gtattggaat aaaagacaaa aaggatggtt
     1141 taatggaata caacacacta gcttcttaca tgcatgtgca ctttgcaaga tcaaacactg
     1201 caaaaaaatt tgtccaaaat tgtattaaaa attcaagaag atgatttttc ttttagaagt
     1261 ttaaagagtg cattaacgat tgctgaagca catggactgc cgcctttgcg acctttattt
     1321 gtaatgtaag gtatttctag agtttgtaat tcttcttttg attctgttgc acatacaaag
     1381 cctactggta ttcctatcac aagagctggt tttgttatgc cttcttttat catgtttatg
     1441 acttctacta gagcagttgg tgcattacct attgcaacta tgccaccatt catctccaaa
     1501 gcagacatgc gcatagaaat ttgtgcacgt gttttgttta gcttttttgc ttcattggca
     1561 atctcaagtt ctgatatttt gcagattgct ttattgccaa actctttaag attttcttta
     1621 ttaaaaccac ctataacacc atttacatcg actattatat tacaaccatt tcttagtgcc
     1681 aaaatgccgt tttgtattgc atttttgtga aatatcatca tattttgtct tgcaaaatca
     1741 aagtctgctg ttgcgtgtat tattcttcta acaatcggcc actcatcttt attataggag
     1801 tgtgaaccaa tttcactttc tataatttcc atactcttat cttcaatgga ttgacctttg
     1861 ttagtaatca tttttctact tcctttgctc tttctattac tagatctatc atcttgtcat
     1921 ctgttccaag atgttctgtg acaaagatat tactcaattt tgatttttct attgcaggat
     1981 taagatctgt gtagatgtct ctttttacat gtgccccttc atgtaaaaag taaaacatca
     2041 caaccaatgc ttttggtttg tttctcacac atttttcaat tccttgtgca atatttggtt
     2101 cttcgatctc taaaaaacag taatccacat ttctatatga tccagataaa tcgtcgacta
     2161 catatttgat tgacatctgc gcattaggat ccttgcttcc atgacatatt atcaaaacgt
     2221 ctatttcttt ttttggtagt gatatgctat tttttacaag agtggatctt atctttttat
     2281 ccacaagttc aaccattgat ttatgtaaag tcataggctt tgaaatgact aatttgacct
     2341 tggtttttga ttgcatagct atagctgcat ttacagcagc tttgaccttt tttccaggat
     2401 acagaaaata tggcactatg gtaagtgcat ctatttcatg tttaagacac ttttctattc
     2461 cttcttctat aaaaggtgga attacctcta aaaaacaata attgcaaaaa agatagtttc
     2521 cttttgtttt gacttgttcg caaatatatc taagctcatc ttgcacttcc ttctctctac
     2581 ttcctctgtc tattaacaaa agtcctcgtt tcattttagg atccttgcta ttgctatggt
     2641 caaattaggt ggaaatttct gtttctccac tatcagtttt cctccagagc tcctcaaagc
     2701 agaagcttca gatacactcg gggttccttc aaaagcctgc acagtttctg atggatttgg
     2761 tatggttatt tgtgcaagtt catccttttc ataataatct acaggaacat ttagctcatt
     2821 tgctaaatct tgtaatcctt taacatttgt ttctttttta attgatgcaa atcgcgcaat
     2881 tgattttgga ctaatattga attttttcat acaatgattc agaccttcca tgatagtgtc
     2941 tcttgttgta tcccaatgta gtccaacacc tatcaccaaa gtctttggtc tgtatacgac
     3001 tgcattcttt agtaattctt ttttctcaat gattctgtct gaaattatca aaaatccctt
     3061 gcaatctgaa ctttccaatt cttccaaact gttgtatatc gttacatttt ttggaagctc
     3121 tttttttggt aaccaccact ctttctcccc cgaatcttgg aaaactccta tcttctcttc
     3181 atttaccata aacgcactaa cttttgtcac tatactgtcg tcatctatct tccaaccgaa
     3241 ttctttacca acaagatcaa ctgcaatggt cttgtttaca tctgcagcag tagttataat
     3301 tgcagtagca ttcaaaactc tagcaatttc ttctgcaagt tgatttgctc ctcctaaatg
     3361 gcctgaaagt gtgctgataa caaacttacc ggtatcgtct attactatca ctgcaggatc
     3421 agttttttta tctttcatat gaggcgcaat taatctaatc acagcaccaa gtgaaaatac
     3481 gcatacaagc gcatcatttg ttttgaataa ttctccaatt tttaccgtag tagaatcatc
     3541 aaaccattct acttctggat tattgctaga aaattttgat ggcgcataaa tcttccatcc
     3601 agaaaattga ttctttaagg ataatcctat gttgattcca ttttttgtga tcgcaaggac
     3661 tgcaactttt tccacaattt gtttttttat cagactaaag atatctgtta ctgtttaggg
     3721 tgttttccta ttattttgcc ttcaaaatca aacataatga catcaatttc aaattcgttt
     3781 tcggagtgat ttcttaattg tttgtaaact tcgaaacaaa ctcgatcaaa aaatccttct
     3841 agtttttctc ttactattat ttcaaaaaca tgtcttgccg tattggcttt ttttatctca
     3901 tgcacaattt ttacatctgc attacattgt tgtgcaagct ttgatagaaa ctccatgtct
     3961 acctttgaac cttttacatg agtctgtttt acccccattg ccatcttggt aagtttgcca
     4021 ataaaacctg caacatatgc ctttttgatc ttcttttttg cgcattgttg cactgcatat
     4081 cctgaaaaat cccccatctg tacaaaacaa tgatctggca aattaattat ggtccttgca
     4141 aaatcctcgc ttcttccacc ggttgttaat acaacagtat catttcccat ggcaattgta
     4201 acatcaaggc tttgtctgat cgatgcggca aaagacgcag tagaatatgg aatcactatt
     4261 ccacttgtgc ctaaaattga tatgcctccc aatattccaa gtcttggatt gtctgtcttt
     4321 ggagctaact cctttccctt tggaacagaa attactatgt ttatgccatt cttttttaga
     4381 atgtcttttg ctacgtctaa aatattttca gatatcatct tttttggtgt tggatttata
     4441 gctgcagaac ccacatcaag tccaagacct ggttttgtaa ctcttccaac tccttctccg
     4501 ccattgatct ctatctgatt tggtttgttg gtaagaaata cttctgtcca tatttcagca
     4561 ccatgagtta catcggggtc atcacctcca tcctttatga ccatacattt ggcactctta
     4621 ctgttgaatt gacagtttga aatttttaca tcgataaatt tttttttagg aagaattact
     4681 tttacagaat caatctttgc ttgattcatt attgacaaaa taccggcttt tgctccagca
     4741 gtagcacaag tgccagtagt aaaaccagtc ctaagctttc cttttgatgc aatctcattt
     4801 tccatagaga agattgattt tttacatatt aatccttatc agatttattt caatattcca
     4861 gatagaatta tccagagcgc aatagcacca agacctccat acaacaaaac atgtgtagta
     4921 atatgtgatg ttggcttttc ttggttaagt tcataggctt cattaacatt cacgccaaag
     4981 gactgccaaa atttatcaaa atttgtcttt gctgaaaact gatattcttc atttgtagcc
     5041 ttatcataat atttccaaaa gtcggtatat gttgattcta tgtctgcatc tgattttttc
     5101 tgactttgtt tttcctcgcg tctaagaaac tggtatgcct caataaccgc tttaaatttt
     5161 tcaccatctt tttcatcttt atttctatct ggatggtact tgagagagag gtttctatat
     5221 gccgtcttta tttccttttg tgatgcacct ttctgaatac ctaaaatctc atagcatttt
     5281 gatgaattca acacaatgtt ttaccacacc cttaaaatta atttgtcgga tatagctata
     5341 gcatgcacaa taaatcatta acaattgcaa taactggtgc taacggtttt gttggtagaa
     5401 atataggaaa aatatttgca aaaaaaggaa tcaagataat tggaataata agaaaaaata
     5461 aaaaatcttc tattactttt ggaattccag taatttcaga tgatctctct gaaaaatacc
     5521 ttatttctag attaaaagga tgttctgcac tattgcactt gattggacag ggaagacaaa
     5581 cagttgattc tgattatgaa aaggtgaacg taggattaac aaaaaatgct atacgtctat
     5641 gtaaaaaggc cggaattaaa aagattattt acataagtgg atttggagt
//
LOCUS       DATIDL010000065         3942 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrosopumilaceae archaeon isolate SMAG_U16342
            k141_2703864, whole genome shotgun sequence.
ACCESSION   DATIDL010000065 DATIDL010000000
VERSION     DATIDL010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935836
            Sequence Read Archive: SRR25158326
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosopumilaceae archaeon (soil metagenome)
  ORGANISM  Nitrosopumilaceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrosopumilales;
            Nitrosopumilaceae.
REFERENCE   1  (bases 1 to 3942)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3942)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/04/2024 05:09:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,520
            CDSs (total)                 :: 1,474
            Genes (coding)               :: 1,474
            CDSs (with protein)          :: 1,474
            Genes (RNA)                  :: 46
            rRNAs                        :: 1 (5S)
            complete rRNAs               :: 1 (5S)
            tRNAs                        :: 43
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3942
                     /organism="Nitrosopumilaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2703864"
                     /isolate="SMAG_U16342"
                     /isolation_source="soil"
                     /db_xref="taxon:2202732"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1296)
                     /gene="thrC"
                     /locus_tag="VLB45_02940"
     CDS             complement(<1..1296)
                     /gene="thrC"
                     /locus_tag="VLB45_02940"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HSD04690.1"
                     /translation="MQMHAYQKCIDPKCGLEYPISDIRIECKRGHLLDVKYQSTPPPS
                     LKEVFYQRRNHANNIFNESGVWRFRDLLNFCQIDTEDIEQCAKHLVSLDGAEGRLSKP
                     YHMSKVADFTGLEHENLWLQPEGYNPSGSFKDNGMSTAVTHAKLVNIKKIICASTGNT
                     SASAAMYAANENIECDVYIPAGEIAPGKLSQAFQFGAQVIEIKGNFDDALSASLQKAG
                     EVGGYTVNSINPFRIEGQKTIVFRALERLDWNPPDWIVYPGGALGNTSSCGKSLMELY
                     EWGWIKKIPRIAVVNSEGANTLHTLYNGKFEDTNLRWNKGNPDTEIIQRYYSHMDKTG
                     MKPKTKATAIQIAKPANILKGLRALEFTNGVVTQVSDQEMMDGMSLVGLNGFDCEMAS
                     GAVPAGIKKLVEEEIIKKDDTVVGILTGRQKDSSIPVEYH"
     gene            complement(1552..1893)
                     /locus_tag="VLB45_02945"
     CDS             complement(1552..1893)
                     /locus_tag="VLB45_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04691.1"
                     /translation="MITQEITVNGKKFNLRLTDQSIMDARRLKALYHAAYEDPESFEE
                     VSSAISNTINQLATAVEPEVSGDDLDGVIQAIMKVVEEKAKEAMDAKNKPAESQKMPK
                     STGKSTKKPKK"
     gene            1971..2870
                     /locus_tag="VLB45_02950"
     CDS             1971..2870
                     /locus_tag="VLB45_02950"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HSD04692.1"
                     /translation="MERKVAEKAHRYFVTGISMITSDGKLGKNVMAAEWTMQISYDPM
                     LIAVFVHESAATFKNIRETKEFGVNVASDKQTSLVNIAGGYSKTEIDKLDIKNSFNLI
                     KSNHIKPPMIDGCIVNAECKLVTMKKLGDHTMIVGKVVSIKHDATKKPLIYHTGRYYQ
                     IGTVIEPFRQILKVNDKTFEWFSSESGEKFVLKCVGAIIRSGKKIVVSSSSSDKNAYE
                     TIPYVIPNRGTDYYDAIITYLKKSGLNITLKQKPILKRIIIKNKKKFQRINFVLFEGR
                     LRSNTHVKLKNVKSDIILESIVN"
     gene            complement(2867..3226)
                     /locus_tag="VLB45_02955"
     CDS             complement(2867..3226)
                     /locus_tag="VLB45_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016940357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04693.1"
                     /translation="MSFENFVDERSLVSHDTFNTKEIKIKLLEVEDDVSDHNWKFGVR
                     IKVKKILVTIKHLQTQQIEEAEFDLKEIEKEMKEKRHYSSTNRWVQTSDVKNGYILST
                     RHTLLMSDAIALDYIVI"
     gene            complement(3249..3875)
                     /locus_tag="VLB45_02960"
     CDS             complement(3249..3875)
                     /locus_tag="VLB45_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HSD04694.1"
                     /translation="MYRIKEHLKHTTLIKTTGSTKLPKSRVPTDTTISSEHNKKVART
                     RRQRGYNWEDTLVKRFNALEEWKAFRLGSPSVALPDILAVSTNENTIFTIEAKSGTGT
                     TLHVPYDQITRCLKWIHTFELYKTRKMIIAFKFLSKKRIGTGVYERRQLREYYKTWDE
                     SNEITDFVCTYEGETYALVNGKRQKLELDDCFMPFKSKHQKQKHRILL"
BASE COUNT         1142 a          740 c          658 g         1402 t
ORIGIN      
        1 atggtattct acaggaatgc ttgaatcctt ttgccttcct gtaaggattc caaccaccgt
       61 atcatctttt ttaattattt cttcttcaac cagtttttta attcctgcag gtactgcgcc
      121 tgatgccatt tcacaatcaa acccatttaa ccctactagc gacatgccat ccatcatttc
      181 ttgatccgaa acctgagtaa ctacaccgtt tgtgaattct aatgctctta atcctttgag
      241 gatgtttgca ggttttgcaa tttggatcgc agttgctttt gtttttggct tcatcccagt
      301 tttatccatg tgcgaatagt atctttgtat tatttccgta tctggattgc ctttattcca
      361 acgtagattt gtatcttcaa actttccgtt gtaaagtgtg tgcagcgtat ttgctccctc
      421 tgaatttact actgcaattc ttggaatttt ttttatccat ccccattcat ataattccat
      481 taggcttttt ccgcagctag aagtatttcc aagagctcct ccaggataga caatccagtc
      541 tggcggattc cagtccaagc gctctaaagc tctaaacact attgtttttt gtccttctat
      601 cctgaatgga ttgatagaat ttactgtata tcctccaact tctcctgctt tttgtagtga
      661 tgctgagagt gcgtcatcaa aatttccttt tatctcgatg acttgagcac cgaactgaaa
      721 tgcctgactt agtttaccag gtgcaatttc cccagctggt atgtacacat cacattcaat
      781 attctcattt gcagcataca ttgcggcaga ggcagatgtg ttgccagttg acgcacaaat
      841 tattttttta atatttacaa gctttgcatg agttactgca gttgacatcc cattgtcctt
      901 aaacgaacca cttggattgt atccttctgg ctgtaaccaa agattttcat gctccagtcc
      961 agtaaaatca gctacttttg acatgtgata tggttttgat aaacgtcctt ctgcaccatc
     1021 caatgatact agatgtttag cgcattgctc aatgtcttca gtatcaattt gacaaaaatt
     1081 taatagatct ctgaacctcc agactccgct ttcgttgaag atattatttg catgatttcg
     1141 tctttgataa aatacttctt taagggatgg tggtggcgtg gactggtatt ttacatcaag
     1201 caagtgacca cgtttacatt ctattctaat gtctgagatt ggatactcta aaccacattt
     1261 aggatcaata catttctgat aagcatgcat ttgcattatt tgaaaagtga cccggtactt
     1321 atttaaatct aaatacttga aaatttaaca cagaaacaag caggaactat ttttttggcc
     1381 ctgatctagt gtgaaaattg atgcaacact tgcactcttt tttaatgcat tcatcttctg
     1441 atacatggcc acaaatgcca cactcacaat gcgtttgcat gtgtctaaat acgttttacg
     1501 cgatataacc catatggcga gtatttttct gatttgtgac tcaataattt gttacttttt
     1561 tggttttttt gtggacttgc ctgtggattt aggcatcttc tgactctccg caggtttgtt
     1621 ttttgcatcc attgcctctt tggccttttc ttccacaact ttcattattg cttgtattac
     1681 tccatctaga tcatctccag agacttctgg ttctacagca gtcgcgagtt gattaatggt
     1741 gttagatatg gctgagctta cttcttcaaa actctcagga tcctcgtaag ctgcatggta
     1801 caatgcttta agccgtcttg cgtccatgat cgattgatcg gttagcctca gattgaattt
     1861 ttttccattt acagttatct cctgagtgat catgtttgaa ttttcaatcc tgctttcata
     1921 aaggtttctg tacaaagaac taataacaga tcaggtgaaa tcagttttta ttggaaagga
     1981 aagttgctga aaaagctcac agatattttg tgaccggcat cagcatgatt actagtgatg
     2041 gaaaacttgg aaaaaatgta atggcggctg aatggaccat gcaaatctct tatgatccaa
     2101 tgcttatcgc agtttttgtg catgaaagtg ctgctacatt caaaaacata cgtgaaacaa
     2161 aagagtttgg ggtaaatgtt gcgtctgaca agcaaacatc gctcgtaaat atagccggag
     2221 gatattctaa aactgaaatt gataaactag atataaaaaa ttcatttaat cttatcaaat
     2281 caaaccatat caaacctcca atgattgatg gttgtatagt caatgcggag tgtaaacttg
     2341 taactatgaa aaaactgggt gatcacacaa tgatagttgg aaaggttgtt tcaataaaac
     2401 acgatgcgac caaaaaacca ctaatctatc acactggaag gtactatcaa atcggcaccg
     2461 tgattgaacc atttaggcag atccttaaag tgaatgataa aacatttgaa tggttttctt
     2521 cggaatccgg tgaaaaattt gttttaaaat gtgttggggc aataatcaga tctggtaaaa
     2581 aaatagttgt gtctagtagc agttcagata agaatgcgta tgaaacaatc ccgtatgtca
     2641 taccaaatag aggtactgat tattatgatg ccattataac atacttgaaa aaatcaggat
     2701 tgaatataac gttaaaacaa aaaccaattc tgaaaagaat aattattaaa aacaaaaaga
     2761 aatttcaaag aattaacttt gttttatttg aaggcaggct tagatcaaac acacatgtaa
     2821 aattgaagaa tgtaaaatca gacattatac tagaatcaat tgtaaattaa atcacaatat
     2881 aatccaacgc gatagcatca ctcatcaaaa gagtatgtct tgtgcttagt atgtatccat
     2941 tttttacatc ggatgtttga acccatctat tagttgagct atagtgtcgc ttttctttca
     3001 tttctttttc tatttccttg agatcaaatt ctgcttcttc aatctgctgt gtttgtagat
     3061 gctttatagt tacaagaatt tttttaactt taattctgac cccaaacttc caattatgat
     3121 cagatacatc atcttcaact tcaagcaatt taattttgat ttcttttgta ttgaacgtat
     3181 catgacttac aagactacgt tcatcaacaa aattttcaaa actcatattc ttgtttcata
     3241 tttagtcatt aaagaaggat tcggtgtttt tgtttttgat gttttgattt aaacggcata
     3301 aaacaatcat caagttcaag cttttgcctt ttcccattaa caagtgcata agtctctcct
     3361 tcataagtac aaacaaaatc agttatctcg tttgactcat cccacgtctt gtaatattct
     3421 cgcagttgac gacgttcgta tactcctgta cctattcgtt tttttgagag aaatttgaag
     3481 gcaattatca tttttcttgt tttatacaac tcaaatgtat gaatccactt taaacatcga
     3541 gttatctgat catatggaac atgtagtgtt gttccagtac cggattttgc ctcaatggtg
     3601 aaaattgtgt tttcgtttgt acttactgcc aaaatgtcag gtaatgctac acttggtgaa
     3661 cctaatctaa atgccttcca ttcttctaat gcattaaatc gttttactaa agtatcctcc
     3721 caattgtatc ctcgctgtct acgtgttctt gcaacttttt tattgtgttc agaagaaatt
     3781 gtagtatctg taggaactct cgatttgggc aattttgtag aacctgttgt tttaattaat
     3841 gtcgtatgtt ttagatgttc ttttattctg tacatggcta tcttcgaatt ctcaacgtat
     3901 agaattcatg gtaattatga aattaccagt gtaaatcggt tt
//
LOCUS       DATIDL010000066         4759 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrosopumilaceae archaeon isolate SMAG_U16342
            k141_485397, whole genome shotgun sequence.
ACCESSION   DATIDL010000066 DATIDL010000000
VERSION     DATIDL010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935836
            Sequence Read Archive: SRR25158326
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosopumilaceae archaeon (soil metagenome)
  ORGANISM  Nitrosopumilaceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrosopumilales;
            Nitrosopumilaceae.
REFERENCE   1  (bases 1 to 4759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/04/2024 05:09:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,520
            CDSs (total)                 :: 1,474
            Genes (coding)               :: 1,474
            CDSs (with protein)          :: 1,474
            Genes (RNA)                  :: 46
            rRNAs                        :: 1 (5S)
            complete rRNAs               :: 1 (5S)
            tRNAs                        :: 43
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4759
                     /organism="Nitrosopumilaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_485397"
                     /isolate="SMAG_U16342"
                     /isolation_source="soil"
                     /db_xref="taxon:2202732"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            181..936
                     /locus_tag="VLB45_02965"
     CDS             181..936
                     /locus_tag="VLB45_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008297979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HSD04695.1"
                     /translation="MSDLKDELHQQMIQAIRHIVDDKKIGIAFSGGVDSSLLAKLCTD
                     LGYDVILLTIGFENSHDIEFSKEIAKTLGSDHKIEKISPKEFPKIAKKIKQTIKTENL
                     SWNENCIAFYHVAKLAKSNKIKKILTANGIDELFCGYNGYRDAIKENEDTVIKLMESK
                     IENEVNMMKAVNAVVSEFDVQILQPFLSKDFITFAKKIPLSHKIHNSDDLMRKHIVRE
                     LAFQVGVPKEASFKRKKALQYGTLIHKTLIKIK"
     gene            complement(929..2290)
                     /gene="purB"
                     /locus_tag="VLB45_02970"
     CDS             complement(929..2290)
                     /gene="purB"
                     /locus_tag="VLB45_02970"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HSD04696.1"
                     /translation="MPILPIDGGRYGTKEMRDIFDEQKKINYQLEIEAAAAEAQAEIG
                     MIPKDAAQNIAKMARSGKITAKRVKQLEAKSDHDTAALVESLGEICKPNAKPWIHYGL
                     TSNDLVDTSNSMQFRDALVIIEPKVAKLAIILAKLAIKFKDVPAVGRTHGQHASIISF
                     GLKFANWALEISKHIERIEEHKKRILICKTLGVVGTGSLMGAKALEVQKRVAKKLSLY
                     PADVTTQVVPRERYAEYIFCIALIGSTLEKIAVEIRNLQRTEIGEVAEPFKKGQMGSS
                     AVPVKRNPVKSERITSLSKMLRSQISVSFENIPLWHERDLSNSANERFILPTSSILLD
                     EMLEAMTKILSDLYVNKDKIKKNLGITKGQIFAEFVLEALIKKGVPRFIAYRDIQRIA
                     FAASDSGAEFLHAVKNDKAISAHLNEKEIEHIFVPGNRLGASGAIIKNVYNIVTKTCS
                     KFI"
     gene            2391..2528
                     /locus_tag="VLB45_02975"
     CDS             2391..2528
                     /locus_tag="VLB45_02975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04697.1"
                     /translation="MVDKRENKEFEESSESYETRTKEKEPDFDGKALSDLYRHTTKLI
                     K"
     gene            2582..4033
                     /locus_tag="VLB45_02980"
     CDS             2582..4033
                     /locus_tag="VLB45_02980"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HSD04698.1"
                     /translation="MTSLVPKKIGNMEYRIEVDSNRGMKVPVTIYADEGLLQKMMTDR
                     TITQAINVSTLPGVQKHVVVLPDGHEGYGFPVGGVAAMDAEEGVISPGGVGYDINCGV
                     RLLRTNLNEKDVRPKLRDLVTELFNSIPSGVGSKGAVKLNFSQLDEVLVKGVRWAIDN
                     GYGTKDDADVCEENGQMKNADPSKVSATARKRGAPQLGSLGSGNHFLEVQKVAEIHDK
                     EAAKRMGITHEGQVTVLIHCGSRGFGHQVCSDYLRVSEQALNKYGISLADRELACVPN
                     SSEEGESYRKAMFAALNFAWSNRQMITHWTRKAFERVFKQSESDLDMKLVYDVAHNIA
                     KVEKHKIDGQLKSVVVHRKGATRAFPGGRDEIPQKYRELGQPVFIPGSMGTASWILLG
                     KPNSLELSFGSTAHGAGRTMSRSAARRSYTDTQVQKTLGDRGIFIKALTREGVVEETP
                     EAYKDVDAVANVSHELGIATKVAKLVPIGVIKG"
     gene            4030..4482
                     /locus_tag="VLB45_02985"
     CDS             4030..4482
                     /locus_tag="VLB45_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HSD04699.1"
                     /translation="MSEEDKELELLKARRLLEMQKNISQQQKQEELKLLKEKQKTSAP
                     DPREILIKHLGYRGLEVLQAAESQFPQETRLVVVKLAELLQSGEVTETIDGGKLLALF
                     RSIGIPVRVHTTINVEQDGKLVSWSDKLKETASIKDEPEQQSDDPNIT"
     gene            4562..>4759
                     /locus_tag="VLB45_02990"
     CDS             4562..>4759
                     /locus_tag="VLB45_02990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04700.1"
                     /translation="MSNRIVIFEISTTKYESDRELVSSVMKDLQLSCGVTDGFLLSEP
                     IEKFGWSFFKILFKNELLVGMQ"
BASE COUNT         1590 a          815 c          913 g         1441 t
ORIGIN      
        1 acaaaatctt tgtgtaattt accgttaatt acactccaac ctggcaaatt gcctatttct
       61 tttttgatct gttcttctgt tagctttgtc agacccatat tgaaatctat caattactcc
      121 tattttaatt gtaaaaacag cgcaactaac ttagggcggt ttttagcatc tttatgcaaa
      181 atgtcggatc ttaaagatga attacatcaa caaatgattc aagcaatacg ccatattgtg
      241 gatgataaaa aaataggaat agcattctct ggtggtgtag atagttctct tcttgcaaaa
      301 ctctgtaccg atcttggata tgacgtaatt ttgcttacta taggatttga gaattctcat
      361 gatatagaat tttcaaaaga aatagccaaa acgcttggta gtgatcataa gattgaaaaa
      421 atctcaccaa aagaatttcc aaaaatagca aagaagatca aacaaacaat aaaaactgaa
      481 aacctgtctt ggaatgagaa ctgtattgca ttttatcatg tagccaagct tgcaaagagt
      541 aacaaaatta agaaaattct tactgcaaat ggaatagatg agctcttctg tggttataat
      601 ggatatagag acgcaataaa agaaaatgag gatactgtca taaaactaat ggaatcaaaa
      661 attgaaaatg aagttaacat gatgaaagct gtcaatgccg ttgtatcaga atttgatgtg
      721 cagatattac aaccgtttct ttcaaaagat tttataacat ttgcaaagaa aattccactt
      781 tcacacaaaa tccataattc tgatgatctt atgcgcaaac acatagttcg agaacttgct
      841 tttcaagttg gtgtgccaaa agaagcatca tttaagcgca aaaaagctct tcaatatggt
      901 actcttattc acaagactct gataaagatc aaatgaattt agaacaagtt tttgttacta
      961 tgttgtagac gttcttgatt atggcacctg acgcaccaag gcgatttcct ggaacaaaaa
     1021 tatgttcaat ctctttttcg ttaagatgtg ctgatatggc cttgtcgttt tttactgcgt
     1081 gcaaaaattc tgctccagaa tcagatgcgg caaaagcaat tctctgaata tctcggtacg
     1141 cgataaacct aggaactcct tttttgatta atgcctccag cacaaattca gcaaaaatct
     1201 gtccttttgt tattcccaaa ttctttttaa ttttatcctt gttgacataa agatctgaga
     1261 gaatttttgt cattgcctct aacatctcat ctagtaagat ggaacttgta ggaagaatga
     1321 atctctcatt tgcagagttg gacaagtctc tttcatgcca taatggtatg ttctcaaatg
     1381 atacgctgat ctgactgcgt aacatttttg agagtgaagt gatgcgttca cttttgacag
     1441 ggtttctttt tactggaacc gcactgctgc ccatctgacc tttcttgaat ggttctgcaa
     1501 cttcgccaat ctcggttctc tgtaagtttc taatctctac tgcaattttt tccagagtgg
     1561 aaccaatcaa ggcaatacaa aatatgtatt ctgcatatct ctcacgtgga acaacttgag
     1621 tagtaacatc tgctggatac agagaaagct tctttgcaac tcttttttgc acttcaagtg
     1681 cttttgcgcc cataagtgaa cctgttccaa caactccaag tgtcttacaa attaaaattc
     1741 gttttttgtg ttcttctatt ctttctatgt gttttgatat ctctaaagcc cagtttgcaa
     1801 attttagacc aaatgatatt atgcttgcat gttgtccatg ggtccttcct actgcaggaa
     1861 catccttgaa ctttattgca agctttgcca aaatgattgc tagttttgca actttgggtt
     1921 ctatgattac aagcgcatct cgaaactgca tagaattact tgtatctacc aaatcattgc
     1981 ttgtcaaacc ataatgtatc catggttttg cattgggttt acaaatctct ccaagtgatt
     2041 caaccagtgc agcagtatcg tgatcgcttt ttgcttctaa ttgtttaact cttttagcag
     2101 taattttacc ggatcttgcc atttttgcaa tgttttgtgc agcatcttta ggaatcatgc
     2161 cgatttctgc ttgagcttct gcagctgccg cttctatctc aagttgataa ttgattttct
     2221 tttgttcatc aaaaatatca cgcatttctt tggtgccata acgtccacca tctattggca
     2281 aaataggcat agtaaatttg aatcaaataa ggaaaataaa cctactcaaa actcattcaa
     2341 agtctataag tgatatctca taaagtaaca atatatctga tttgaggaaa ttggtagata
     2401 aaagagaaaa caaggaattt gaagaaagct cagaatctta tgaaacaaga accaaagaaa
     2461 aagaaccgga ttttgatggc aaagcactaa gtgatttgta taggcacaca acaaaactaa
     2521 tcaagtgaac tcttttgctt atgatttaaa ttgtgtagaa gagcgatttt tgtagattca
     2581 gatgacatca ctcgttccaa aaaaaattgg taacatggaa tatagaattg aagttgactc
     2641 taatcgtgga atgaaagttc cagtaacaat ctatgcagat gaaggattgt tacaaaaaat
     2701 gatgacagat agaacaatta ctcaagcaat caatgtttct actttgccag gtgttcaaaa
     2761 acacgtagta gttctacctg atggtcatga aggatacggt tttccagtgg gcggagtagc
     2821 tgcaatggat gcagaagagg gagtgatcag tccaggaggt gttggttatg atatcaattg
     2881 tggtgttaga ttgttacgaa caaatctgaa cgaaaaagac gttagaccaa aactaagaga
     2941 tcttgtcaca gaacttttca attcaattcc atctggagta ggttcaaaag gtgcagtaaa
     3001 actgaatttt tctcagcttg atgaagttct tgtaaaagga gtgagatggg caatagacaa
     3061 tggatatggt acaaaagatg atgcagatgt atgcgaagaa aacgggcaaa tgaaaaatgc
     3121 agatcctagc aaagtatcag caactgcaag aaaacgtggc gcaccacaac ttggaagtct
     3181 tggctctgga aatcattttc ttgaggtaca aaaagttgca gagatacatg ataaggaagc
     3241 tgcaaaaaga atgggaataa cacacgaagg gcaggtcact gttttgattc attgcggttc
     3301 cagaggcttt ggtcatcaag tatgtagtga ttatcttaga gtatcagaac aggcactcaa
     3361 caaatatgga atatctctag ctgatagaga gctagcatgt gttccaaatt cgtcagaaga
     3421 aggtgaatct tacagaaaag caatgtttgc agcactaaat tttgcatgga gtaataggca
     3481 gatgataact cattggacaa gaaaggcgtt tgagcgtgtt ttcaaacaat ctgaatctga
     3541 tttggacatg aaacttgttt acgatgttgc tcataatatt gctaaagtag aaaaacacaa
     3601 aatcgatggc caactaaaat ctgttgtggt tcatagaaaa ggtgccacta gagcattccc
     3661 aggaggacga gatgaaatac cacaaaaata tagagaacta ggtcagcctg tttttattcc
     3721 aggttctatg ggaactgcta gctggattct tcttggcaaa ccaaattctt tagaactgag
     3781 ctttggttca acagcacacg gtgctggcag aacgatgtca agatctgccg ctcgtagaag
     3841 ctatactgac actcaagtcc aaaagacact tggtgacagg ggaattttca taaaagcgct
     3901 tacaagagaa ggcgtagtag aagagacacc tgaagcgtac aaagatgtag atgctgttgc
     3961 aaatgtatcc catgagcttg gcattgctac caaagtagca aagcttgtgc caataggtgt
     4021 gataaaggga tgagcgagga agacaaagaa ctagaacttc tcaaggcaag gcgtttgctt
     4081 gagatgcaaa aaaatatttc acaacaacaa aaacaagaag aattaaaact attaaaagaa
     4141 aaacaaaaaa caagtgctcc tgatccaaga gagattctaa taaaacatct tggttatcgt
     4201 ggtttggaag ttctacaggc tgcagaatca caatttccac aggaaactag gcttgttgta
     4261 gttaagctgg ctgaacttct tcaatctgga gaggttactg aaacgattga cggtggaaaa
     4321 ttactcgctc ttttcagatc tattggaatt ccagtccgtg ttcataccac aataaatgta
     4381 gaacaagatg gaaaacttgt ttcttggtca gataaactaa aggaaaccgc ttcgattaaa
     4441 gatgaacctg aacaacaatc tgatgacccg aacatcacat gattgatgtc tatatctcga
     4501 tctattgttt acaaaaataa attttcgaaa tatagattta gtagctaaat aataaaacat
     4561 catgagtaac cgcatcgtaa tttttgaaat ttctactaca aaatacgaaa gtgatagaga
     4621 acttgtttct tcagtaatga aagatcttca attatcttgt ggagtaactg atggtttttt
     4681 gcttagtgag ccaattgaaa aatttggttg gagtttcttt aagatattat tcaagaatga
     4741 attacttgta ggtatgcaa
//
LOCUS       DATIDL010000067         5622 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrosopumilaceae archaeon isolate SMAG_U16342
            k141_81079, whole genome shotgun sequence.
ACCESSION   DATIDL010000067 DATIDL010000000
VERSION     DATIDL010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935836
            Sequence Read Archive: SRR25158326
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosopumilaceae archaeon (soil metagenome)
  ORGANISM  Nitrosopumilaceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrosopumilales;
            Nitrosopumilaceae.
REFERENCE   1  (bases 1 to 5622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5622)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/04/2024 05:09:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,520
            CDSs (total)                 :: 1,474
            Genes (coding)               :: 1,474
            CDSs (with protein)          :: 1,474
            Genes (RNA)                  :: 46
            rRNAs                        :: 1 (5S)
            complete rRNAs               :: 1 (5S)
            tRNAs                        :: 43
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5622
                     /organism="Nitrosopumilaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81079"
                     /isolate="SMAG_U16342"
                     /isolation_source="soil"
                     /db_xref="taxon:2202732"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..445
                     /locus_tag="VLB45_02995"
     CDS             <1..445
                     /locus_tag="VLB45_02995"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HSD04701.1"
                     /translation="KVWDIVSDLDREPKFWHNISKVRNLSTEGNVVKREITMGKGDRC
                     LQTVTLYPKDKVLAEFTKGIINGTKTIALTSVGSGTSLEIAWNIKFTGGAGFLKGMLI
                     KSFQQQTDDVVQLIKQESETGRPAESTMQMEERKHWADLIDSDKK"
     gene            complement(454..618)
                     /locus_tag="VLB45_03000"
     CDS             complement(454..618)
                     /locus_tag="VLB45_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007401843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04702.1"
                     /translation="MGLFKKKESKNETKCKECGLELHDPERLERHMKKAHAHVSQKNF
                     DEPSGGGGLW"
     gene            complement(649..828)
                     /locus_tag="VLB45_03005"
     CDS             complement(649..828)
                     /locus_tag="VLB45_03005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04703.1"
                     /translation="MKGMDYKKFRESEKEHFLTKDGRFTKTEVIKQMGSWLNQNKSAE
                     PEDFLENYQVKEDKN"
     gene            complement(849..1064)
                     /locus_tag="VLB45_03010"
     CDS             complement(849..1064)
                     /locus_tag="VLB45_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04704.1"
                     /translation="MKASKYLPKIGTFDGAGFWKKAYAHQRGNLLRQVKVPDDQIKIL
                     VNKTYQELPPPLRFEIETSGIKEQDLK"
     gene            complement(1092..1400)
                     /locus_tag="VLB45_03015"
     CDS             complement(1092..1400)
                     /locus_tag="VLB45_03015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04705.1"
                     /translation="MDEYLDEEGLLKVIRTFELSEEITKLNWTWNDRSNPVREAHDLL
                     AKCQKLFLEISEYEQRMGDKLNKYQRNKIEDAVTDLGKLIPYIKNKIKPYESLENIGR
                     "
     gene            1709..1927
                     /locus_tag="VLB45_03020"
     CDS             1709..1927
                     /locus_tag="VLB45_03020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04706.1"
                     /translation="MNIYDMKSIHCCKCDKFIGELDYDAVVVLPKCGRCADPLPEGDD
                     KVLYIVNKYNKNPQSLVSSLKAGSLLDW"
     gene            2292..2948
                     /locus_tag="VLB45_03025"
     CDS             2292..2948
                     /locus_tag="VLB45_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008299149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04707.1"
                     /translation="MKNLCYFMMIFVVIVGGAMQTSSADLIVRGNEHAPIYVSDTDSG
                     RFDFSNDGLDDYYVKAHYTGNEKQTYKVDYKIQDECVDGATYDSATMKIGFTKQGNPW
                     WIWIVENIDSWNPWFKSARSTDANHRIDLVDLPDGVIPIPYPSSGDDVIVGDSKSNSG
                     SFVHRDSIAELDGQSGWDGSIFFSAPAGEYILHTIHPSEGISGCDRLAALGIPIIIKP
                     "
     gene            complement(2966..5158)
                     /locus_tag="VLB45_03030"
     CDS             complement(2966..5158)
                     /locus_tag="VLB45_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor EF-2"
                     /protein_id="HSD04708.1"
                     /translation="MKYKATDQILKIIGNKKNIRNFGVIAHVDHGKTTMSDSLLASSG
                     IIAPSVAGQALALDSMKLEQDRQMTIVQANVTLLYEKDGQEYVINMIDTPGHIDFTGR
                     VTRSLRAIDGAVVVSDSVEGIMTQTETVTRQALEERVRPVLYINKIDRLIKELRLTPD
                     KMQEWLVEIITNFNRLIDIYAEPEYKEKWKVSIQDGSVSFGSAKDRWGFNADVMKKKG
                     ISFKDIYTAYSEGGDLKALAEKAPLYDAVLGMVVKHHPSPDVAQKYRIPKIWKGDLES
                     DIGKALLNCDDNGPAVMMIVNMTMDPAAGPVAIGRLFSGRLKDGDRIHIIDSKREGRI
                     QSVNFFMGNQREMVGELAAGNIPALLGLEHARAGQTLSSVADTHMFEGIQYVSEPVVQ
                     IAVEPKHPKDLPKLVEALRRITIEDPNLVVKINEESGETVIAGMGVLHLEIASSLIAD
                     AGVQVVTSQPLINYRETIRTAAGPVMSKSPNRHNKIFMKVEPLDEKVAEMIRNGTLNE
                     YKDEKEVAEILRNAGWEREDAKRVMRFDPRGNVMVDGTKGVQFVKESTDSILSGFEDT
                     MKEGPLTREQVRGCKFTFHHFVPHEDPAHRGLSQLGPASRRVCMGAMLMAQPVLLEPT
                     LGIEVRVPQEMIGNVAGVISGKRGKVLNMEQKGIIHILTGEIPASETFDLSEVMRGQT
                     AGRAMWNTHFKAWTPVPNSVLPTIMGEIRKRKGLSPEPPKAEEFIDSE"
     gene            5361..>5622
                     /locus_tag="VLB45_03035"
     CDS             5361..>5622
                     /locus_tag="VLB45_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MqnA/MqnD/SBP family protein"
                     /protein_id="HSD04709.1"
                     /translation="MEITLGHTPDADDAFMFYGMLTGKVTSPDFKVNHVVEDIETLNK
                     RALKHELDVTAVSVHACAYTPNYTILRSGGSFGKGYGPIIISK"
BASE COUNT         1550 a         1112 c         1089 g         1871 t
ORIGIN      
        1 caaggtctgg gatattgtat ctgaccttga cagggaaccc aagttttggc ataacatcag
       61 taaagtaaga aacctttcca cagaaggaaa tgttgtaaag cgtgagatca caatgggcaa
      121 aggagacagg tgtttgcaaa ctgtgacact gtacccaaaa gacaaggttc ttgcagagtt
      181 tacaaaggga ataatcaatg gaacaaaaac catagcattg acatctgttg gaagtgggac
      241 ctcgcttgaa attgcatgga acatcaaatt cactggaggc gcaggttttc tcaaagggat
      301 gctgataaag agttttcagc aacagactga cgacgttgtg cagctaataa aacaagaatc
      361 tgaaaccggc aggcctgcag aatcaacaat gcaaatggaa gaaagaaagc actgggcaga
      421 ccttattgat tctgacaaaa aatagattct gacctaccac aatccgccgc caccgctagg
      481 ctcatcaaag ttcttttgtg agacatgagc gtgagccttt ttcatgtgcc tttctaggcg
      541 ctctgggtca tgcagttcta agccgcattc cttgcatttt gtctcgtttt tggattcttt
      601 tttcttgaaa aggcccatgg tatgatcaat atttttccga tattaaaatt aattcttatc
      661 ttcttttact tggtaatttt caagaaagtc ttcaggttct gctgacttgt tttggtttag
      721 ccatgaaccc atctgtttta tgacttcggt cttggtaaat ctgccatctt ttgtaagaaa
      781 atgctctttt tctgattctc tgaatttttt ataatccata cctttcattg ttttcttgat
      841 gtaatgatct attttaaatc ctgttctttt attccacttg tttctatttc aaatcttaat
      901 ggagggggca attcctggta tgtcttgttt accagtatct tgatttgatc gtctggaacc
      961 tttacctgtc taagtaaatt cccgcgctgg tgggcatatg ctttcttcca aaacccagca
     1021 ccgtcaaacg ttcctatctt tggaagatat ttggaggctt tcaagttttt aggtggttag
     1081 ccattctatt tttatctgcc tatgttttct aaactttcat aaggtttgat tttgtttttt
     1141 atataaggaa ttagttttcc taaatctgtc acggcgtctt caattttatt tcgctggtac
     1201 ttgttaagtt tatcacccat tctttgctcg tattctgaaa tttctagaaa gagtttttga
     1261 cacttggcca aaaggtcgtg tgcttctcta actgggtttg aacgatcgtt ccaagtccag
     1321 ttgagttttg taatttcttc agataattca aaagtcctta tgactttaag aagtccctct
     1381 tcatcaagat actcatccat gtctgattag acatttgaaa accgttttta agtatttgta
     1441 cacattcaac aaaaacgtct catgtagttg atcatgacag aaaatttaga aaaatgacga
     1501 tttttgaact aatctgactc tctctaaatt tcagagctta ttgtgccagt gttttactat
     1561 tgtataccaa atgatgttct ttccgcactg ttataagcga tggacactac tattatccat
     1621 gttagaagct attaaacaaa tcaaaataga aataatgcga aatttaggaa atcttaacaa
     1681 aacattttct tcctaggagt gactgaaatt gaatatctat gatatgaaat caatccattg
     1741 ctgcaagtgt gacaagttca taggagaact tgattatgat gcagtagttg tacttccaaa
     1801 gtgcggtcga tgtgccgatc cattgccaga aggcgatgat aaagtactat acatagtgaa
     1861 caaatacaac aaaaatccac aaagtttagt gtcatcgcta aaagctggtt cacttttgga
     1921 ttggtaaaag aaattgagtt actacaaacg aaaactgtgt gaggattgta ggagagttaa
     1981 ttgtacgaaa ggatgcaact gtgactgcca ctatgagcca aggggctagc gctttaacag
     2041 gtttttgtat gatatggtaa tgaattcttt accgataata ctgtaatatt ttttggtaaa
     2101 caatttatca tatctggtat atcataatga agaccataga actccaaggg atgatgttaa
     2161 ttacggcaat cctttgacct atggagacgc tgtggtcact aaattattta caattagaat
     2221 atgataaaaa tgcaaaccat ctgtgaaaat aatctacaag aattataagc gtgagtgagt
     2281 tacttgtgct catgaagaat ttgtgttact ttatgatgat tttcgtcgta atagtcggag
     2341 gtgccatgca gacttctagt gcagacctta ttgttcgtgg taatgaacat gctccgattt
     2401 acgtatctga tacagacagt ggccgatttg attttagtaa tgatggtctt gacgattatt
     2461 atgtaaaggc tcactatacc ggaaatgaaa aacagacata caaagtagat tacaaaatac
     2521 aagacgaatg tgtggatggt gctacatatg attctgctac aatgaagata gggtttacaa
     2581 aacaaggaaa cccatggtgg atttggatag tagaaaacat tgattcatgg aatccgtggt
     2641 ttaaatcagc tcgcagtacc gacgcaaatc acagaattga tcttgtcgat ttacctgatg
     2701 gggtcatacc aattccatac ccatcaagtg gagacgatgt gattgtggga gattcaaaaa
     2761 gcaactccgg ttcatttgta catcgcgact ctattgcaga gttggacggt cagtcaggat
     2821 gggatggttc tattttcttt agtgcgccag ctggtgaata tatcttgcat acgattcacc
     2881 cttccgaagg aatatctggg tgtgacagac ttgccgctct tggcattcca ataatcataa
     2941 agccttgaga taaaattttt gtattttatt cgctgtcgat aaattcttct gcttttggag
     3001 gttctggact caatcccttg cgttttctaa tctctcccat aatagtaggc aagacagagt
     3061 ttggaaccgg cgtccatgcc ttaaagtgag tgttccacat tgctcttcca gctgtttgac
     3121 ctcgcattac ttcggacaaa tcaaaagttt cagaagctgg aatttctcca gtaagaatgt
     3181 ggatgattcc tttttgttcc atgtttagga ccttgcctct ttttccagat attactcctg
     3241 caacatttcc aatcatttct tgtggaactc tgacttcaat tccaagtgtt ggttctagca
     3301 aaactggttg tgccattagc attgcaccca tgcatacacg tcttgatgca ggaccaagtt
     3361 gtgaaagacc tctgtgagct gggtcttcgt gtggtacaaa gtgatggaat gtaaacttgc
     3421 atcctcttac ctgttctctt gtaagcggac cttccttcat ggtatcttca aatcctgata
     3481 aaatggaatc agttgattct ttaacaaact ggacaccttt tgtgccgtct accattacgt
     3541 ttcctcttgg atcaaagcgc attacacgtt ttgcatcttc tctttcccat cctgcatttc
     3601 tcaaaatttc tgcaacctct ttttcatcct tgtattcgtt taatgttccg tttctgatca
     3661 tctctgcgac tttttcatca agcggttcta ctttcataaa gatcttgttg tgtctgtttg
     3721 gtgattttga catcacagga ccggcagctg tcctgattgt ttctctataa ttgatcaaag
     3781 gctgtgaagt gacaacctgt actcctgcat ctgcaatcag cgaagatgca atttcaaggt
     3841 gcaatacacc catgcctgca atgactgtct caccgctttc ttcgttgatt tttaccacaa
     3901 ggttggggtc ttcaattgta attcttcgca aagcctctac aagcttggga agatcctttg
     3961 gatgctttgg ctctactgca atctgtacga ctggttctga aacatactgg attccttcaa
     4021 acatatgagt atctgcaaca cttgaaagtg tctggccagc tcttgcatgt tccaatccca
     4081 aaagtgctgg aatgtttcct gctgcaagct cgccaaccat ttctctttgg ttgcccatga
     4141 aaaagttcac cgactggatt cttccttctc tttttgaatc aatgatgtga attctatcgc
     4201 cgtctttgag tctgcctgaa aataatcttc caatagctac aggacctgca gctggatcca
     4261 ttgtcatgtt tacaatcatc atcactgccg gaccgttatc atcacaattc aaaagtgcct
     4321 taccgatatc tgactctaaa tctcctttcc aaatttttgg aatcctgtat ttttgtgcaa
     4381 catcaggaga tggatgatgt tttacaacca ttcctaaaac tgcatcatac agtggagcct
     4441 tttctgcaag agccttgagg tctccacctt cagaatatgc agtgtaaatg tccttgaaag
     4501 aaattccttt ctttttcatt acatctgcat tgaatcccca cctgtctttt gcagaaccaa
     4561 atgaaacgct gccgtcttgt atgctgactt tccatttttc tttgtattcg ggttctgcgt
     4621 aaatgtcaat taatctgtta aaattggtaa tgatttctac aagccattcc tgcatcttgt
     4681 ctggagtgag cctgagctct ttgatgagac gatcaatctt gttgatgtat aatacaggtc
     4741 tgactcgctc ctcaagggct tgtcttgtta ctgtttctgt ctgtgtcatg attccttcca
     4801 ccgagtctga aacaacaact gctccatcta tggctctaag acttcgcgta acacgtcctg
     4861 taaagtcaat gtgccctgga gtgtcaatca tattaatgac atattcctga ccatcttttt
     4921 cgtaaagcaa ggtaacgttt gcttgtacaa ttgtcatctg tctgtcttgc tctagtttca
     4981 tggaatcaag tgcaagggct tggcctgcaa cggatggcgc aatgattcca cttgatgcca
     5041 aaaggctgtc gctcatggtt gtctttccgt ggtctacgtg agcaatgact ccaaagttcc
     5101 taatgttttt cttgtttcca atgatcttta ggatctgatc tgttgccttg tacttcatga
     5161 atgaaaaaaa tcgatctaga gccggtatta aaccttatca agaattgagt ttgatctgtt
     5221 tttgataaat cgtcaaagat ctggcatttg actttttgac aaaaatttca tctgcttttt
     5281 gatttctttt tcaaatgcaa ttgttgatat gacacatttt tcgtaatttg atatttataa
     5341 tagacaccaa aagtatgctt atggaaatta cacttggcca cactcctgac gctgatgatg
     5401 ctttcatgtt ttatgggatg ctgacaggca aagtaacatc tcctgatttc aaagtaaatc
     5461 atgtagtaga agacattgag acgctaaaca aaagggcgct aaaacatgag cttgacgtga
     5521 ctgcagtttc agtccatgcg tgcgcataca ctccaaatta tacaatactg agaagtggtg
     5581 gcagctttgg caagggctat ggtccaataa taatatcaaa aa
//
LOCUS       DATIDL010000068         2969 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrosopumilaceae archaeon isolate SMAG_U16342
            k141_1091915, whole genome shotgun sequence.
ACCESSION   DATIDL010000068 DATIDL010000000
VERSION     DATIDL010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935836
            Sequence Read Archive: SRR25158326
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosopumilaceae archaeon (soil metagenome)
  ORGANISM  Nitrosopumilaceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrosopumilales;
            Nitrosopumilaceae.
REFERENCE   1  (bases 1 to 2969)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2969)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/04/2024 05:09:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,520
            CDSs (total)                 :: 1,474
            Genes (coding)               :: 1,474
            CDSs (with protein)          :: 1,474
            Genes (RNA)                  :: 46
            rRNAs                        :: 1 (5S)
            complete rRNAs               :: 1 (5S)
            tRNAs                        :: 43
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2969
                     /organism="Nitrosopumilaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1091915"
                     /isolate="SMAG_U16342"
                     /isolation_source="soil"
                     /db_xref="taxon:2202732"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..494)
                     /locus_tag="VLB45_03040"
     CDS             complement(<1..494)
                     /locus_tag="VLB45_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonyl-tRNA synthetase editing
                     domain-containing protein"
                     /protein_id="HSD04710.1"
                     /translation="MRILQLHCDNIEFTPIKKEIKSAEEVEPSTKKIDEVVVAFIAVE
                     EGDNSIVAKKAIAEIKESMKKVGCSKLLLYPYAHLSSKLASPSVALSVLKEMETLVGD
                     LEVSKAPFGWTKSYKIQVKGHPLAENSKTITSTEKEDTTSDAVKAESKIESFWYIMST
                     DGKM"
     gene            789..2027
                     /locus_tag="VLB45_03045"
     CDS             789..2027
                     /locus_tag="VLB45_03045"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSD04711.1"
                     /translation="METNEANTHKRDIIIIEDSLTVATLLRDFLHKLGYQNIHICENG
                     ATGIRIFDEFVKVGKSPLVFLDYGLPDMEGGEVMKEILSISPDTKIILETASEKTEES
                     IKDAIRHGAYNYLGKPIRFEDVKNIIDTFEKEDTIAQHPEPATQKQIEALMSKPPQAM
                     NLKLTAAVLITGFVIVTFLGLFAYQTFSPVPMSTPITTGRPIIENLKGDVIDTWVAWR
                     LIEGDSLHIIILNSEIIPDDKFNSIKNAILSEETLKIENSLVDKLPQESSSVYYKGWK
                     GALEKASESNVVYYIPTNFDVIKGEKAIGEVSIILSEIEAGDGSLAFTRSIADENEHQ
                     LLKSFITVFDVKNLSNEQMEAIIRHEFGHALGLAHSTASEDLMYTTIKTKYPYISECD
                     VDAIVSLYDEKKTNLVICEN"
     gene            2134..>2969
                     /locus_tag="VLB45_03050"
     CDS             2134..>2969
                     /locus_tag="VLB45_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008612 - peptidyl-lysine modification to
                     peptidyl-hypusine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase family protein"
                     /protein_id="HSD04712.1"
                     /translation="MDHHHFRGKDVPHFKINPNMGVDDLVEIFASSGYNARQLGEAAK
                     LYCKMIEDDATICLTVSGAMTPVGFGGLFKSLIERGFVDWIIATGANVYHEDHFAWNL
                     PIKQGHFEVDDMILYEKDIVRIRDVYIKGKETLMAEDKIVQQMFKNELINKPFTTAEF
                     CNMMGKYSKQKSKHPEKSFVVAAYDYDVPVYVSTLKDSSLALDLAPLRLANKIYNLDF
                     VREILEQAAIVYSSKKSGIVEIGGGVPKNTAQQTGPLLDQILGLGHGGQNYIIQITDA
                     RP"
BASE COUNT          970 a          524 c          544 g          931 t
ORIGIN      
        1 tgcatctttc catctgtaga catgatgtac caaaatgatt ctatttttga ttctgctttt
       61 accgcgtctg aagtggtatc ttctttttct gtagatgtta tggtttttga attttcagcc
      121 aatggatgac ccttgacttg aatcttgtat gactttgtcc atccaaacgg tgcttttgat
      181 acttcaagat cacctaccag tgtttccatt tcttttaaaa ctgaaagtgc aacgcttggt
      241 gatgcaagct ttgagctaag atgtgcataa ggatacaaaa gcaatttact gcatccaact
      301 tttttcatag attctttaat ttcagcaatt gccttttttg caacaatgga attgtcgcct
      361 tcttctactg caataaatgc tacaacaact tcatctattt tttttgtaga tggttctact
      421 tcttccgctg attttatttc cttttttata ggtgtaaatt ctatattatc acaatgaagc
      481 tgaagtatgc gcatgttttt tctctgtctt tgatattgaa ttaaatgtta tggctataac
      541 cttcttagtg aagaattatt ctaacttgaa ctttaacaaa caattgcatt ttctgcatac
      601 tcgcatttat taattgcata attttttaga caaaagttac attcatctcg atctggaatt
      661 tgttctcact ctgtattccc ctaacgttta agcgcctctg cgtctattgt atgattatta
      721 ggtctgataa tacgcatata tcgttttatg tgaaaaaatt caatactgtg ataaagtagc
      781 tgcatacaat ggaaacaaac gaagcaaaca ctcacaaaag agatatcatt ataattgaag
      841 acagtctgac agtagccact cttttacgtg attttttaca taaactaggt tatcaaaata
      901 tccatatttg tgaaaatggt gcaactggta tcagaatttt tgacgagttt gtaaaagtag
      961 gaaaatcccc acttgtcttt cttgattatg gtttgccaga tatggaagga ggtgaggtca
     1021 tgaaagagat attatctatc agcccagata ctaaaattat actagaaact gcaagtgaaa
     1081 aaaccgaaga atctatcaaa gacgccataa gacatggcgc ctataactat cttggaaagc
     1141 cgattcgatt tgaagacgta aaaaatatca tagatacttt tgaaaaagag gataccattg
     1201 cacagcatcc tgaacctgca acacaaaaac aaatcgaagc tcttatgtca aagcctccgc
     1261 aggctatgaa tctaaaactt actgctgcag ttttgataac aggttttgtg attgttactt
     1321 ttttgggatt atttgcatat cagaccttct cacctgtccc tatgtctaca ccaataacaa
     1381 caggaaggcc aataatagaa aatctaaaag gtgatgttat agatacttgg gtggcttggc
     1441 gtttaattga aggagactca cttcatatta ttatattaaa ttcagagatt attccagatg
     1501 ataaattcaa ttcaatcaaa aatgcaattt tgtcagagga aacattaaag atagaaaatt
     1561 cacttgtgga caaactacca caagaatctt catctgttta ttataaaggt tggaaagggg
     1621 cacttgagaa agcatcagaa agcaatgttg tatattacat cccaacaaat tttgatgtga
     1681 tcaaaggcga aaaagctata ggcgaagtat caataattct gtcagagatt gaggcaggag
     1741 atggctcgtt agcttttaca agatcaatag cagatgaaaa tgagcatcaa cttttaaagt
     1801 catttattac ggtctttgat gtcaaaaatt tatccaacga acaaatggaa gcaataatta
     1861 gacatgagtt tggccatgcg ctgggtcttg cacattctac tgcctctgaa gatctaatgt
     1921 atacgactat taaaacaaaa tatccttaca tttcagaatg tgacgttgat gctatagtgt
     1981 cattatatga cgagaaaaag accaatctgg tcatatgtga aaactaactc tcaaatgact
     2041 atgctcgagg atgcttcttt agatctgtaa tcttatcccc tgatcttttt gttcctagca
     2101 agtattattt agcccctaca aagatcaccg tacgtggatc accatcattt tcgtggaaaa
     2161 gacgttccac actttaagat aaatccaaac atgggtgtgg atgaccttgt agaaattttt
     2221 gcaagctcag gttataacgc gcggcagctt ggtgaggctg caaagcttta ctgcaaaatg
     2281 attgaagatg atgcaacaat atgtcttaca gtttctggtg caatgactcc tgtaggcttt
     2341 ggtggattgt tcaaatcatt aattgagcga ggatttgttg actggattat tgctacgggg
     2401 gcaaacgtgt accatgaaga tcactttgca tggaatcttc caatcaagca aggacatttt
     2461 gaagttgatg acatgatatt gtatgaaaaa gacattgtaa gaatcagaga cgtatacatc
     2521 aaaggaaaag aaaccctgat ggcagaagac aaaatcgtac aacagatgtt caaaaatgaa
     2581 ttaatcaaca aaccgtttac aactgcagag ttttgcaata tgatgggaaa atactcaaaa
     2641 caaaagtcaa aacatccaga aaaaagcttt gttgttgctg cttatgatta tgatgtccca
     2701 gtctatgtct ctaccctgaa agactcttcg cttgcacttg atctggcacc attaagactt
     2761 gcaaacaaaa tatacaatct ggattttgtt agagagatac tagagcaagc tgccatagtg
     2821 tatagttcta aaaagtctgg catcgtagaa ataggaggtg gggttcctaa gaacactgca
     2881 cagcaaacag gccctctctt agaccagata ctagggctag gtcatggcgg tcaaaattac
     2941 atcatacaga taactgatgc aagaccaga
//
LOCUS       DATIDL010000069         3446 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrosopumilaceae archaeon isolate SMAG_U16342
            k141_2708164, whole genome shotgun sequence.
ACCESSION   DATIDL010000069 DATIDL010000000
VERSION     DATIDL010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935836
            Sequence Read Archive: SRR25158326
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosopumilaceae archaeon (soil metagenome)
  ORGANISM  Nitrosopumilaceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrosopumilales;
            Nitrosopumilaceae.
REFERENCE   1  (bases 1 to 3446)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3446)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/04/2024 05:09:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,520
            CDSs (total)                 :: 1,474
            Genes (coding)               :: 1,474
            CDSs (with protein)          :: 1,474
            Genes (RNA)                  :: 46
            rRNAs                        :: 1 (5S)
            complete rRNAs               :: 1 (5S)
            tRNAs                        :: 43
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3446
                     /organism="Nitrosopumilaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2708164"
                     /isolate="SMAG_U16342"
                     /isolation_source="soil"
                     /db_xref="taxon:2202732"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..627)
                     /locus_tag="VLB45_03055"
     CDS             complement(<1..627)
                     /locus_tag="VLB45_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HSD04713.1"
                     /translation="MSSESYLVGLGNDDSETRKKASSDFTSFWASQAKNLHWFEEWKS
                     TLQWDAPFAKWFVGGKLNASYNTLDVHQQKRATKPAILWEGENNDRQVLTYNDLWIQV
                     NKFANVLKSLGVTKGDRVTIYLPMVPELIIAMNACARIGATHTVVFSGFSARSLRDRI
                     EDSKSKVIITADGGFRRGTVVKLKQVIDDAIQGFDFVQHVIVLERTKIP"
     gene            complement(709..1542)
                     /locus_tag="VLB45_03060"
     CDS             complement(709..1542)
                     /locus_tag="VLB45_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdvA-like protein"
                     /protein_id="HSD04714.1"
                     /translation="MSKDEIEFIGKQVKDMYGTFIGKVVGTITDIDGSVQTVGVDCGS
                     EGLKQLPFEQLVVQGAFVIYIPRWRLEAQRLLREKGLTLRRLHALMDIVSENDDMKED
                     AELIHEKYKMKLLTLEESEKQIKEKLEQRIKELDSQFQSIRMLVFDAKLQFKSNEISD
                     AKYESVKTQTVEIIEHIEHEKAEIARIQQKIADLSVESVERTESPKQELHTAAISYLG
                     KVENTPVPQPAQVAAAPTSGTPVGTNTPQTPTQRPVAIGADGAEEDSQDSDWLRRISS
                     Q"
     gene            1692..1967
                     /locus_tag="VLB45_03065"
     CDS             1692..1967
                     /locus_tag="VLB45_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04715.1"
                     /translation="MIGKEELDRILLFVAKRWTDMSRDPKNKAFESLPSGFWMNSVGG
                     SAGPGKWITMLMYTEDESEAASFKEVLLRWQSKHAEKPSDLIQINKK"
     gene            2000..>3446
                     /locus_tag="VLB45_03070"
     CDS             2000..>3446
                     /locus_tag="VLB45_03070"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HSD04716.1"
                     /translation="MSEFSDAEKEILSKHFSNVNDSVFAVITPKQVDRGALMSRYSRS
                     DKSMRKIFLDEFLQNENRGEEFYNRVLLEYGDDSVAELGEAQIAIEGLSNIAVKKIED
                     RRIGLSYLEKSSRYVSWDKKINGEYKFFRESDIMSSNFADTYLESCNLDFDVYSKNIE
                     PMIKFIQEREPIGNQKFKNSSGDEVLFSRLTDETDIKSATRIYNATIKAKALDVLRGL
                     LPASTLTNVGITGNGRAFEYLLGILYGSSLIEERILASKLHRELDVIIKSFVKRADDK
                     YGKALQKYHHDLKNAAGSLAKKYLKDKKKTDFLVELVDSDPSRKAEEKIISAILYEQS
                     QGISYKTILAEVRKMSPTIRKKIIKAFANMRENRRHRPPRAFEMTEYTFDLLTNFGMF
                     RDFHRHRVLTLERQLLTTNHGFSVPSEIRNLGIEKDYLDCMYKSKEVFDLIKPKMQEQ
                     SQYVVNFAYNYPYFMRLNLREATHLIELRTVP"
BASE COUNT         1059 a          657 c          671 g         1059 t
ORIGIN      
        1 aggaattttc gttcgctcca aaacaataac gtgttgcaca aaatcaaaac cttgtatagc
       61 atcatctatg acttgcttta attttacgac agtacctcgc ctgaatccac catctgcagt
      121 gattattact ttggatttcg agtcttcaat tctatctctt aatgatcttg cactaaaccc
      181 agaaaagacg acagtatgtg ttgcaccgat tcttgcacat gcattcattg ctataatcag
      241 ttcaggaacc attggaaggt agattgttac tctatcgcct tttgtaactc caagagattt
      301 tagtacattt gcaaacttgt ttacctgaat ccatagatca ttatatgtaa gaacttgtct
      361 gtcattgttt tcgccttccc acaaaattgc aggttttgtt gcgcgttttt gttggtgaac
      421 atcaagtgtg ttgtatgaag cgtttagctt tccacccaca aaccacttgg caaaaggagc
      481 atcccactgc aaagtgctct tccattcttc aaaccaatgc aggttttttg cttggcttgc
      541 ccaaaatgag gtaaaatcag aacttgcctt ttttcttgtc tcagaatcat catttcccaa
      601 gcctaccaga taactctcag aactcacagg cagagtttct tttttgtcaa aataaaactt
      661 gcttaaaaaa ggaggctagg agcgaacctg ttatttccta ttcagttgct attgggacga
      721 tatccttcta agccaatctg aatcttgaga atcttcttct gcaccatcag ctccaatggc
      781 tactggtctt tgtgtcggtg tttggggagt attagtacca acaggagtac cagaggtcgg
      841 tgctgctgca acttgggctg gttgcggaac tggtgtattt tctaccttac ccaaatatga
      901 aattgcggcg gtatgaagtt cttgtttggg gctttctgta cgctccacac tttccacgga
      961 aagatctgcg attttttgtt gaatgcgtgc tatctctgcc ttttcgtgtt cgatgtgttc
     1021 tattatttct acagtctggg ttttaacaga ttcatacttg gcatctgata tttcgttact
     1081 cttgaattgt agctttgcat caaacaccag cattctaatc gactggaatt ggctatccaa
     1141 ttctttgatt ctctgctcca gcttttcttt gatttgtttt tcagattctt ccagagtaag
     1201 cagcttcatc ttgtattttt cgtggatgag ctctgcatcc tccttcatgt catcattttc
     1261 ggatacgatg tccatcagtg catgtaggcg gcgcaatgtc agaccctttt ctcgcaacaa
     1321 tcgctgtgct tctaatctcc atcttggaat gtagatcaca aaagcacctt gcacgacaag
     1381 ttgctcaaat ggcagctgtt tgagtccttc agagccacaa tcaacaccga ctgtttgtac
     1441 agaaccgtca atgtcagtta tggtgccgac gactttgcca ataaaagtgc cgtacatgtc
     1501 ctttacttgc ttgccgataa actcgatctc gtctttgctc attgggtaca ggttttggag
     1561 ttaatataac cagcaaagtg ttaacaaggt ttggaatttt ggtaagatca ttttagctaa
     1621 cccaaaaaag cgaagaaacg aggaaaaatt ttcgcaattt taaaatcatg tcttgtagtt
     1681 tagattaatc catgatagga aaagaagaac ttgacagaat tctattgttt gttgcaaaaa
     1741 gatggacgga tatgagccgc gatccaaaaa acaaagcctt tgaaagtctg ccttctggtt
     1801 tctggatgaa ctccgttggc ggaagtgctg gccctggaaa atggataaca atgctcatgt
     1861 atacggaaga cgaatctgaa gctgcatcgt ttaaagaagt tttattgcga tggcaatcaa
     1921 agcatgcaga aaaaccatct gatctaattc agataaacaa aaagtagatt atttataatt
     1981 gaacaacttt ccgatcgtaa tgtcggaatt ttctgatgct gaaaaagaga tcctgtcaaa
     2041 gcatttttca aatgtaaacg attctgtgtt tgcggtcatt acaccaaagc aagtagaccg
     2101 tggagctttg atgtcaagat attccagatc tgacaagagc atgagaaaaa tttttcttga
     2161 cgagtttttg caaaatgaaa atcgtggtga agaattttac aatagagtat tattagaata
     2221 tggtgatgat tcagtagctg agcttggcga agctcagatt gcaattgaag gactgtcaaa
     2281 tattgcagtg aaaaaaattg aagaccgtag aattggttta tcgtatttgg aaaaatcttc
     2341 gcgatatgtt tcgtgggata aaaaaataaa tggagaatac aaatttttta gagagtcaga
     2401 catcatgagt tctaattttg ctgacacgta tttagaatca tgtaatcttg attttgatgt
     2461 gtattcaaaa aatatagaac cgatgataaa attcattcaa gaaagggaac cgataggaaa
     2521 tcaaaaattt aagaactcaa gcggagatga agttttattc tctagattaa cagatgagac
     2581 agacatcaaa tctgctacaa gaatctataa cgctaccatc aaggccaagg cgctagatgt
     2641 actgagagga ctccttcctg catctacgct tacaaacgtg ggcataactg gaaacgggcg
     2701 tgcctttgag tatctgctag gcatacttta tggctctagc cttattgaag aacgtattct
     2761 tgcatcaaag ctacaccgcg agcttgacgt aataataaaa tcatttgtaa aaagagccga
     2821 cgacaagtat ggaaaagcgc ttcagaaata tcatcatgat ctaaaaaatg cagctggcag
     2881 tcttgctaaa aaatatctta aagacaaaaa gaaaacagac tttcttgtgg aattggtgga
     2941 ttctgatcct tccagaaaag cagaagaaaa gataatttcg gcaattttgt atgaacaaag
     3001 tcaaggaatt tcatacaaaa ccatacttgc cgaagtaagg aagatgtcac caactataag
     3061 aaaaaaaatt atcaaggcat ttgcaaacat gcgtgaaaac agacggcatc gtcctccacg
     3121 agcatttgaa atgacagaat acacttttga tctattaaca aactttggaa tgtttcgtga
     3181 ttttcataga caccgtgttc ttactttaga aagacagtta cttacaacaa atcatggatt
     3241 ttctgttcct tctgaaattc gcaatcttgg gatagaaaaa gactatcttg attgcatgta
     3301 taagtcaaaa gaagtctttg atctaatcaa accaaagatg caggagcaat ctcagtatgt
     3361 tgtaaacttt gcttacaact atccatactt tatgcgatta aatctgcgcg aagcaacaca
     3421 cctgattgag cttagaacag taccac
//
LOCUS       DATIDL010000070         7599 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrosopumilaceae archaeon isolate SMAG_U16342
            k141_1905470, whole genome shotgun sequence.
ACCESSION   DATIDL010000070 DATIDL010000000
VERSION     DATIDL010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935836
            Sequence Read Archive: SRR25158326
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosopumilaceae archaeon (soil metagenome)
  ORGANISM  Nitrosopumilaceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrosopumilales;
            Nitrosopumilaceae.
REFERENCE   1  (bases 1 to 7599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/04/2024 05:09:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,520
            CDSs (total)                 :: 1,474
            Genes (coding)               :: 1,474
            CDSs (with protein)          :: 1,474
            Genes (RNA)                  :: 46
            rRNAs                        :: 1 (5S)
            complete rRNAs               :: 1 (5S)
            tRNAs                        :: 43
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7599
                     /organism="Nitrosopumilaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1905470"
                     /isolate="SMAG_U16342"
                     /isolation_source="soil"
                     /db_xref="taxon:2202732"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(407..2503)
                     /gene="nrdD"
                     /locus_tag="VLB45_03075"
     CDS             complement(407..2503)
                     /gene="nrdD"
                     /locus_tag="VLB45_03075"
                     /EC_number="1.1.98.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HSD04717.1"
                     /translation="MELSEDFGDQKRGGILQSASKRVRMIFSVMASPNRIDILRILNS
                     KGPLTYSELKSLAGFKSKKESGKFAYHLRKLLRQSLVALNKSERRYTITNLGKLVLSL
                     ARQIEERSIIESGKMYVRTSHEAIEEFNSHKIIQSLVREGSLPLELAQKITEEVENRI
                     YKFQTTYLTASLIREMVNSVLLEHGHEDYRHKLARFGLPAYDIHEMITNVDGIYNGVE
                     GLLFKTGQTVFGDHLVLNTLPKDVADSHLSGDIHITNAGIWSLLPDTIFVNIKELIED
                     GIDLRGKFLGVTRIPPIKSLDDLATSLSLLTSLLFKEASQEVVMDGLITILQKYAKNL
                     GEIEEKIASTFATTSTTPKYSKNATLVSFRTPLGSDSKIIGAILAGYKKYVNATPIPH
                     IGLIIDYEKGKVSEFSDILSEIIALGGRVIFTKGQTSNRGIVRGDTKNTGTTSINLQS
                     LSINLPRLAFESNKDETYFRARLALLMKPALAAMSLRKKDISDLTRRGLNPILANNTQ
                     YMQKGTVTLVVNLVGLKEAVLGILGYEDNKAGHEIMTKVLETAIEVAAKKGKEMGDNV
                     TISMTESDAAVRFSTLDGEKYGKMSVHQYVEGDSYSTGLVLNASEVEGMTIKTDKVVE
                     CNQVSKMLNGGLLIQLKISDDMDIPKIKKAIEKASELVGSFRPLRKVPICGNCGLKDE
                     KIGDKCPTCKSPYILA"
     gene            complement(2572..2742)
                     /locus_tag="VLB45_03080"
     CDS             complement(2572..2742)
                     /locus_tag="VLB45_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04718.1"
                     /translation="MIAMSVDVVCGKCGEKIANMKMLKSIKEVIRPYGGKCPSCGQTL
                     SVSEFTVDVQKN"
     gene            complement(2805..3179)
                     /locus_tag="VLB45_03085"
     CDS             complement(2805..3179)
                     /locus_tag="VLB45_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HSD04719.1"
                     /translation="MYNLSAKEEFITNAKLGKILARKCTKCGFLHLATTYFCQNCGNR
                     GFENTLIEGKGSVVTYTIITVPPAGYEKYTPYAWVVITLDGIDLRISGFMHDIKSPAD
                     LPLGTKARVAGYDERGILLEKL"
     gene            complement(3172..4326)
                     /locus_tag="VLB45_03090"
     CDS             complement(3172..4326)
                     /locus_tag="VLB45_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase domain-containing protein"
                     /protein_id="HSD04720.1"
                     /translation="MEKVCVIGAGSTKYGKLQDSITDIAVQASADAIESAGIEPKEIQ
                     AGYISNVFGIADKQVHLGPVIMSNLGISEVPSLSIESACGSGSVSFREAYANVAAGFY
                     DAVLATGVEKVTHTGTEWTTTYFSYCSDFFYEGGSGASFPGLFASMARAYLAEFKATE
                     EDLAMVAVKNHENGFLNPKAHLRKKITIDDVMKSAVVASPLKLYDCCPFSDGASAVIL
                     CNEKFAKAHTKNYIKVIGSGRGGSPAALQGREHITTIPSTKLAAQAAYKMAGIKPKDI
                     DFAEVHDCFTIAELVDTEDLGFFEKGKAVEAVRQGMTKLNGAIPINPSGGLKSKGHPI
                     GATGIGQVVEVFDQLTGKAGERTIKDAQTGLTHNFGATGASCAVHIFKSV"
     gene            4399..4860
                     /locus_tag="VLB45_03095"
     CDS             4399..4860
                     /locus_tag="VLB45_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HSD04721.1"
                     /translation="MQQQKTSQILVTKKNSSVRNSVKITKRKVTKMKNEIKQYYDKNG
                     YLSWSERKKKYVILGTNLPNNGLVECPECHTGKLMVIHSRTTKKRFIGCSNYYNGCRA
                     SSPLIQRGMICATKMSCTLCAWPIILFRYSRKQKWLRQCANLKCRSRQIKV"
     gene            complement(4850..5659)
                     /locus_tag="VLB45_03100"
     CDS             complement(4850..5659)
                     /locus_tag="VLB45_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008300841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HSD04722.1"
                     /translation="MPKVALVQFRADTNKYKNLKKILSYIDQAAKKGAELCAFPEFMM
                     FYTPSSQSGTELASLAEKISGNFVSEISKRAKQNSIQVVGTFYEKSPKLNRVYDTSFL
                     INKNGIIISKYRKIHLYDALGMKESKKLYPGSKIVKPVKSTIGKLGMMICYDLRFPEM
                     SRTLASSGSEILVVPSAWVKGEMKEEHWITINKTRAIENGCYVIAPDQVGNIYCGRSV
                     VIDPYGKILLDMKKKQGIGLVDISTERIKQIRKKLPLLKNRRTDIYTNFKL"
     gene            complement(5695..5871)
                     /locus_tag="VLB45_03105"
     CDS             complement(5695..5871)
                     /locus_tag="VLB45_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase family protein"
                     /protein_id="HSD04723.1"
                     /translation="MPLITVSMYPGRTQEQKEDFAKAITKSAVEILKTKENHVIVVFE
                     ENPKENWYMAGNPL"
     gene            complement(5987..6628)
                     /locus_tag="VLB45_03110"
     CDS             complement(5987..6628)
                     /locus_tag="VLB45_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014966136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Snf7 family protein"
                     /protein_id="HSD04724.1"
                     /translation="MTGFDKTWTRQETRGLSEKIHDAVKPQGALKPRIEGAVNKLQGQ
                     ISKMDSMLGKLRERDAQLFKRIVAAMQQHDASTSKVLSNELAEIRKVTKMLGNARMAL
                     EQVQLRLTTVHDLGDAMVAIGPAVSVMKGLKSSLGRFMPEADAELNSMTQTLNGLMTD
                     SLAGGDFSVDSDVSNEETERILQEASAVAEQQIGDRFPSVPSPSGVSSQSTYE"
     gene            6736..7491
                     /locus_tag="VLB45_03115"
     CDS             6736..7491
                     /locus_tag="VLB45_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014966135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036361 - racemase activity, acting on
                     amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HSD04725.1"
                     /translation="MVKIAVFDSGLGSLSIIKSIRKKMPSDIIYFADQKNFPYGTKSI
                     VRLRKIINSTISMLEKKFNPDLIVVGSNTPSLLLKDVIKRPNIIGVFPPLNNAIKKSK
                     SNSIAILATKSVVESTALKKYIKKYVPKKIKVVKINASPLVELVESGKFIHDKNLCAI
                     KIKWILNPLLEHDVDVVTLSSTHLPFLLSLLKQIFPQVTFLDPADSVADQIVTKINKK
                     SKRSTMKIFTSGNVKIFQKQLSKIGIKNQVMKL"
     gene            complement(7486..>7599)
                     /gene="cbiF"
                     /locus_tag="VLB45_03120"
     CDS             complement(7486..>7599)
                     /gene="cbiF"
                     /locus_tag="VLB45_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214446.1"
                     /note="catalyzes the formation of cobalt-precorrin-5 from
                     cobalt-precorrin-4; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-4 C(11)-methyltransferase"
                     /protein_id="HSD04726.1"
                     /translation="RTAIIIIGDIVKPKSYEYSKLYDKTFSHGFRKAKIKS"
BASE COUNT         2260 a         1428 c         1283 g         2628 t
ORIGIN      
        1 tagtttgtct gccaaactat ggtctggcct attacaagca acaagtgcct tgtatacggc
       61 atttgaaatt ttgtccttct ggaagtttaa cactcgtcca tcgcgctttt taacttcaat
      121 gatcgagttt tctatctgtg aaaagtctgt caaacgtatc cccgtaaccg taagaaaata
      181 gagtctaata aactctctgg caaaaaatat ttttctaaaa ttgatttttg tatcaatttt
      241 gttgacaccg ttacaattat catagtattg gatcagaaca atcccaaata aaaaccaaaa
      301 aaatttaata aaaatttcag aacaatttga tcgcattttt cagttttttt gaaaaaagaa
      361 tcagaataga aggagcaaat gaaaacttgc gacgattagc gcaaggttat gccaatatat
      421 atggagattt gcaggtagga catttgtctc cgatcttttc gtcttttagt ccacaatttc
      481 cgcagatagg aacctttctg agtggcctga aagaacctac taattcactt gccttttcta
      541 ttgctttttt gattttaggt atatccatgt catcagatat ttttagttgg atcaatagtc
      601 cgccgtttaa catctttgac acctggttac attctactac tttgtctgtc tttattgtca
      661 ttccctcaac ttctgaagcg tttagtacca aacctgtaga ataagaatca ccttctacgt
      721 attgatgcac tgacatcttg ccatattttt ccccatccaa ggttgagaat cttactgctg
      781 catcactttc tgtcatggat atagttacgt tatcgcccat ttcttttccc ttcttggctg
      841 caacttctat ggcagtttca agtacttttg tcattatttc gtgtcctgcc ttattgtctt
      901 catatcccaa tatgcctaaa actgcttcct ttaggcctac tagattgact acaagcgtaa
      961 ctgttccctt ttgcatatac tgagtgttat ttgcaagaat aggattgagt ccacgtctag
     1021 taagatccga tatgtctttc tttctaagag acattgctgc aagtgcgggt ttcattaaga
     1081 gtgcaagacg tgctctaaag taagtttcat ctttgtttga ttcaaaggct agtctgggta
     1141 ggtttatgga aagagattgt aagttaattg atgtagtacc agtatttttt gtatctccac
     1201 gcactattcc tctgtttgat gtttgaccct tggtaaagat cacacgtcct cctaatgcta
     1261 tgatttcaga aagtatgtct gaaaattcgc tgactttgcc tttttcatag tcaattatca
     1321 gtcctatgtg tggaatcggt gtagcattga catatttctt gtaacctgca agtatagcgc
     1381 ctattatttt agaatcggat cctaatgggg ttctaaatga tacaagagta gcattcttgc
     1441 tgtattttgg agtggtggat gttgttgcaa atgtacttgc aattttttct tcaatttctc
     1501 ctaggttttt tgcatatttt tgtaaaattg ttatcaatcc atccataact acttcctgtg
     1561 atgcttcttt gaatagtaat gaagtaagta atgaaagcga tgttgcaaga tcatccaagc
     1621 tcttaattgg aggtattctt gttactccaa ggaatttacc tctgagatca attccatctt
     1681 caatgagttc tttgatgttc acaaatattg tatctggtaa gagtgaccag attccagcat
     1741 ttgttatatg aatatctcca gaaagatgtg agtctgctac atcttttggc agagtgttaa
     1801 gaacaaggtg atccccaaaa actgtctggc ctgtcttaaa caataatcct tccacaccat
     1861 tatagatacc atctacattt gtgatcattt catgaatatc gtatgcagga agaccaaacc
     1921 tagcaagctt atgcctataa tcttcatggc cgtgctctag gaggacggaa ttaaccattt
     1981 cacgtataag cgaggcagta aggtatgtag tctggaactt gtagatacga ttttctacct
     2041 cttcggttat cttttgtgcc aattctagag gaaggctacc ttctcgtaca agagattgga
     2101 ttatcttgtg agagttgaat tcttctattg cttcgtgcga agttcgtaca tacatcttgc
     2161 cactttcaat tatagagcgt tcctcaatct gccttgccag acttaggaca agttttccaa
     2221 ggtttgtaat ggtataacgt ctttcagatt tgtttaatgc tacaagggat tgccttagta
     2281 attttcgtag gtgatatgca aattttccgc tttctttttt agatttgaat ccagcaagag
     2341 attttaactc agaataggta agcggtccct ttgagtttaa aatgcgtaaa atatcgatcc
     2401 tgtttggact tgccatgaca gaaaagatca ttcttacgcg ctttgatgca gattgtaaaa
     2461 ttcctcctcg tttttgatcg ccaaaatcct cgctcagctc cataggcatg tctagataag
     2521 catcggtaaa taagatttgt gacgaaaatg atcaccagag atctattttt atcagttttt
     2581 ttgtacgtct actgtaaatt ctgacacgga aagggtttgt ccgcatgaag gacatttacc
     2641 accatatgga cggatcactt ctttgatcga ctttaacatt ttcatgtttg ctattttttc
     2701 accacattta ccacacacga catcaactga cattgcgatc atttttgtat aaaattataa
     2761 aagaactgtt ttgagttttt tttaatcagt tcaataaatt agatttacag tttttctaac
     2821 aagataccac gctcatcgta tccagcaact cttgcttttg ttccaagtgg tagatcagca
     2881 ggggatttga tatcatgcat aaaaccagaa attcgtaaat ctataccatc cagtgtgatt
     2941 acaacccaag catatggtgt gtatttttca tatcctgctg gaggaaccgt aattatcgta
     3001 tatgtaacaa cagagccctt tccttcaatc agtgtatttt caaatcccct attaccacaa
     3061 ttttggcaga aataggtagt agcaaggtgt aaaaaaccac attttgtgca tttacgggca
     3121 agaatcttgc ctaatttggc gttagtgatg aattcctctt ttgctgacaa attatacact
     3181 tttgaagata tgaacagcgc agcttgcacc agttgcgcca aagttgtgtg tcaaaccagt
     3241 ttgagcatct ttaatggttc tttctccggc ttttcctgtt agctgatcaa atacttctac
     3301 tacttgacca ataccagtag cacctattgg atgtcctttt gattttaatc ctcctgacgg
     3361 atttatcggt atagcaccat ttagttttgt cattccttgt ctaactgctt caactgcttt
     3421 tcctttttca aagaatccta aatcttcagt gtcaactagc tctgcaattg taaaacaatc
     3481 atgtacttct gcaaaatcaa tatcttttgg cttaatgcct gccatcttgt atgcagcttg
     3541 agctgcaagt tttgtacttg gaattgttgt gatgtgttct cttccttgta atgctgctgg
     3601 tgatcctcct ctaccagatc caataacttt gatgtagttt tttgtgtgag ctttagcaaa
     3661 tttttcgttg cataaaatca cagcacttgc accatcagaa aatggacaac aatcatacaa
     3721 ttttagtgga ctggcaacaa ctgcagattt catcacgtca tcaattgtaa ttttttttct
     3781 taagtgagct tttggattca gaaatccgtt ttcatgattt tttactgcaa ccattgctag
     3841 atcttcttca gttgctttga attctgcaag ataagctctg gccattgatg caaataatcc
     3901 tggaaatgat gcaccagatc caccttcata gaaaaaatca gaacagtatg aaaaatatgt
     3961 cgtggtccac tctgtgccgg tgtgagttac tttttcaaca ccagttgcta aaaccgcatc
     4021 ataaaatcca gcagccacat tagcgtatgc ttctctaaat gaaacagaac cactgccgca
     4081 tgcagactcg atagacaaag atggtacttc agaaattcca agattactca ttatgacagg
     4141 tccaagatga acctgtttgt ctgctatacc aaacacattt gagatatagc cagcctgaat
     4201 ttcttttggt tctattcctg cactttcaat ggcgtcagct gatgcctgaa ctgcgatatc
     4261 agttatgcta tcttgtaatt tgccgtattt tgtgcttcca gcaccaataa cacagacttt
     4321 ttccacaaat ctcgctgact atattcccta taaaaattct tcactaatat tcaattacaa
     4381 gaaggagtta ggattagtgt gcaacaacaa aaaacatctc aaattttagt taccaagaaa
     4441 aatagctcag taagaaattc tgttaaaatt accaaaagaa aagttacaaa gatgaaaaat
     4501 gaaataaaac aatattacga taaaaacgga tatctgtcgt ggtctgagag aaaaaagaaa
     4561 tatgtcatac ttggaacaaa tttacctaat aatggattgg tagaatgtcc agagtgtcac
     4621 actggaaaat tgatggtgat acattcacga actacaaaaa aaagattcat tggatgttcg
     4681 aattattata atggatgtag agcatcttct cctctgatac aaagaggaat gatttgcgca
     4741 acaaaaatgt cttgcactct ttgtgcatgg ccgataattc ttttcagata ttctaggaag
     4801 cagaaatggt tacgacaatg tgctaatcta aagtgtcgga gtagacaaat taaagtttaa
     4861 agttagtata gatatcagtt cgcctattct ttagtagagg tagttttttt cttatttgtt
     4921 ttattctttc agttgaaata tcaacaagcc caattccttg tttctttttc atatccaata
     4981 atatttttcc atatggatca atcacaacac ttcttccaca atagatattt ccaacctgat
     5041 ctggtgctat aacataacaa ccattttcaa ttgctcgtgt tttgttgatt gttatccagt
     5101 gttcttcttt catttcccct tttacccaag ctgatggcac tacaagaatt tctgaaccag
     5161 atgatgcaag agttctagac atttcaggaa atctcaaatc ataacagatc atcattccaa
     5221 gttttccaat cgtactctta acaggtttta caattttaga acctggatag agtttttttg
     5281 attctttcat tccaagtgca tcgtaaagat gaattttcct gtattttgat ataattatgc
     5341 cgtttttatt tataagaaaa gatgtatcat atactctgtt taacttagga ctcttttcat
     5401 aaaatgttcc gacaacttga atagaatttt gctttgcacg ttttgaaatt tcagaaacaa
     5461 aatttccaga tattttttct gctaaactcg caagttctgt accggactgt gaggaaggag
     5521 tgtaaaacat catgaattct ggaaatgcac ataattccgc accttttttt gctgcttgat
     5581 caatatagct tagaattttt ttgagatttt tgtacttgtt agtgtctgct ctgaattgta
     5641 caagagcaac ttttggcatg aattttttat ataaagttaa aataaaaatg tagtttaaag
     5701 aggatttcca gccatgtacc agttttcttt tggattctcc tcaaagacta caattacatg
     5761 attttctttg gttttgagaa tctctacagc tgactttgta attgctttcg caaaatcctc
     5821 tttttgttct tgggttctac caggatacat tgacacggtt atcaatggca tgcctcacca
     5881 cctgtgtgga tgaatttatt tcttaaggca aatagagacc ataattctga aagtaattag
     5941 atagaaaaag aggacgtatt cgccagtctt cccattgcga gttaccctac tcgtatgttg
     6001 attgtgacga tactccggag ggtgatggaa ctgatgggaa tctgtcgcca atttgttgtt
     6061 ctgcaactgc agatgcttct tgtagaattc tttctgtttc ttcgttagat acatcagaat
     6121 ctacgctaaa gtctccacct gccagcgagt ccgtcattag tccattgagc gtttgtgtca
     6181 tagagttgag ctctgcatct gcttccggca tgaaccttcc aagggatgat ttgagtccct
     6241 tcattactga tactgctggt cctattgcta ccatcgcatc tccaagatca tggacggtgg
     6301 taagacgcaa ctgtacttgt tctagtgcca ttcttgcatt gcctagcatc tttgtgacct
     6361 ttctgatttc tgcaagctcg ttggacaaaa ctttgctcgt actagcatcg tgttgttgca
     6421 ttgcggctac gattcgtttg aaaagctgtg catcacgttc tcttagcttt cctaacatag
     6481 aatccatctt cgatatctga ccctgtaact tgttcacagc tccctcgatt cgtggtttga
     6541 gggctccctg tggttttaca gcgtcatgta ttttttcgct taaacctctg gtctcctgtc
     6601 gagtccaggt tttatcgaag cctgtcatgt gaggtagttt aaaattcgat tcttaatgcc
     6661 cggtgtatat tatcatcaac attaggctaa atttagctca caaaatgtaa atcacacaca
     6721 gattattcta gctttatggt aaagattgca gtctttgatt caggtcttgg ttcactttcc
     6781 attataaaat caattagaaa aaaaatgcca tctgatatta tttattttgc agatcaaaaa
     6841 aactttcctt atggtacaaa atccatcgtc agacttagaa aaataattaa ttcaaccatt
     6901 agtatgttag aaaaaaaatt taatccagat ctaattgtag tcggttcaaa tacgccatca
     6961 ttattactaa aagatgtaat aaaaagacca aatataattg gagtttttcc accattaaac
     7021 aatgcaataa aaaaatcaaa atcaaattct atagctatac ttgcaaccaa atctgttgta
     7081 gaaagtactg cattaaaaaa atatattaaa aaatatgtac caaaaaaaat taaagttgtt
     7141 aaaattaatg catcaccact agtagaactt gttgaatccg gaaaattcat tcatgataaa
     7201 aatttgtgtg caataaaaat taaatggatt ttaaatccac ttttagaaca cgatgtagac
     7261 gttgtaacat tatcaagtac acacctacct tttcttttat cattattaaa acaaatattt
     7321 ccacaagtta catttttaga tccggctgat tctgttgcag atcagattgt aacaaaaata
     7381 aataaaaaat caaaaagaag tacgatgaaa atttttacat ccggtaatgt aaaaatattt
     7441 caaaaacaat tatccaagat tggaattaaa aaccaagtaa tgaaattatg atttaatttt
     7501 tgcttttcta aaaccatgac taaaggtttt atcatatagc ttcgaatatt cataagattt
     7561 tggttttaca atatcaccaa taattataat tgcagtacg
//
LOCUS       DATIDL010000071         2514 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrosopumilaceae archaeon isolate SMAG_U16342
            k141_290178, whole genome shotgun sequence.
ACCESSION   DATIDL010000071 DATIDL010000000
VERSION     DATIDL010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935836
            Sequence Read Archive: SRR25158326
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosopumilaceae archaeon (soil metagenome)
  ORGANISM  Nitrosopumilaceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrosopumilales;
            Nitrosopumilaceae.
REFERENCE   1  (bases 1 to 2514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/04/2024 05:09:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,520
            CDSs (total)                 :: 1,474
            Genes (coding)               :: 1,474
            CDSs (with protein)          :: 1,474
            Genes (RNA)                  :: 46
            rRNAs                        :: 1 (5S)
            complete rRNAs               :: 1 (5S)
            tRNAs                        :: 43
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2514
                     /organism="Nitrosopumilaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_290178"
                     /isolate="SMAG_U16342"
                     /isolation_source="soil"
                     /db_xref="taxon:2202732"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..393)
                     /locus_tag="VLB45_03125"
     CDS             complement(<1..393)
                     /locus_tag="VLB45_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007401847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00725 family protein"
                     /protein_id="HSD04727.1"
                     /translation="MTKKRQILVIGNNENGFPPELEKVAYETGLEVAKSDSVLITGGL
                     GGVMQAACHGAKDAGGITVGIIPQNDSSFANPYCDIVIPTGIGLARDFLNALSADGVI
                     IIGGGSGTLSETCAAYMYKKPIVAIKNTG"
     gene            481..606
                     /locus_tag="VLB45_03130"
     CDS             481..606
                     /locus_tag="VLB45_03130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04728.1"
                     /translation="MEKRSMPICFTTDQYKAIEEIAKRKGMLNASQLIEKVLNEN"
     gene            694..1104
                     /locus_tag="VLB45_03135"
     CDS             694..1104
                     /locus_tag="VLB45_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04729.1"
                     /translation="MGLLHRKPSFCTVCKKSITHKHRPKREWNVEGPLCSDCYINEMQ
                     KHYDLSVKQKCVTCGIEKNVPDLWEPRWQWEMKGLLCKPCFDKKEEDFNKLKNFCSIC
                     GAKLGIIRYNPKKQWEIAGQICRSCWDIQKAKRS"
     gene            1108..1311
                     /locus_tag="VLB45_03140"
     CDS             1108..1311
                     /locus_tag="VLB45_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016939233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C2H2-type zinc finger protein"
                     /protein_id="HSD04730.1"
                     /translation="MHLFKKFKCETCEKKFKQQEELMQHIQVVHGKDTPYDCKQCNES
                     FSSMEQMRTHLQKYHSYKGTRDI"
     gene            complement(1308..1508)
                     /locus_tag="VLB45_03145"
     CDS             complement(1308..1508)
                     /locus_tag="VLB45_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase Rbp10"
                     /protein_id="HSD04731.1"
                     /translation="MASEDTPVVAEEKVQAPTFNVVYSCLRCGTPVSQSELSRLPEIK
                     CICGFRVFNKLRPPVVKTVKAV"
     gene            complement(1549..1857)
                     /gene="rpsJ"
                     /locus_tag="VLB45_03150"
     CDS             complement(1549..1857)
                     /gene="rpsJ"
                     /locus_tag="VLB45_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HSD04732.1"
                     /translation="MTQLARIKLTSTSLPRLDGVCSEIKGIGEKTGVKVKGPTPLPVK
                     RLNVVTRKSPCGQGTNTYEKWEMRIHRRVIDLSADDRAIRQLMRIKIPNDVYIELTLK
                     "
     gene            complement(1866..>2514)
                     /locus_tag="VLB45_03155"
     CDS             complement(1866..>2514)
                     /locus_tag="VLB45_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HSD04733.1"
                     /translation="FDDFKLPEKPTGKPLRIPIQDVYTITGVGTVPVGRVETGVVKAG
                     MKIIVMPAGVPGEIKTIETHHTQMESAEAGDNIGFNLRGVDKKDIKRGDVIGTPDSPP
                     NVAKEFRAQIIVIHHPTAIAPGYTPVMHAHTAQVAATIVAFEAKINPQTGAAEEKDPK
                     FLKAGDSAIVRIRPVRPLPIETFQDFPELGRFALRDMGATIAAGVVKDITEKYTA"
BASE COUNT          743 a          489 c          492 g          790 t
ORIGIN      
        1 gccggtgttc tttattgcca caattggctt tttgtacatg tacgcagcgc atgtttcaga
       61 aagtgttcca gaaccaccac caattattat gacgccatct gctgagagag cattaagaaa
      121 atctcttgcc aatccaattc cagttggtat aactatatcg cagtatggat ttgcaaacga
      181 agaatcgttt tgcggaatta ttccaaccgt tattccacct gcatcctttg ctccatgaca
      241 tgcagcctgc attactccac ctagtccgcc agtgatcaaa acagaatcag atttggcgac
      301 ctccagtcca gtctcatatg ccactttttc aagctcagga ggaaagccat tctcgttgtt
      361 tccgatcaca agaatttgtc ttttctttgt caacatttta cgtaattatt gatgtaataa
      421 aaatcgtgaa tttcagatac caccacaaag gatattaaat gattaagtta aatcagccac
      481 atggaaaaac gttcaatgcc aatttgtttt acaacagatc agtacaaagc gatcgaagag
      541 attgcaaaac gaaagggaat gcttaacgca agtcagctga ttgaaaaagt attaaacgaa
      601 aactagtaca accaacgatt ttctttttat tcaaatctct agtcatgtgt gtttcatgat
      661 atgttttttt aacggaaaac catattttgg gtattgggat tattacatcg taaaccgtca
      721 ttttgtacag tatgtaaaaa aagcataact cacaaacaca gaccaaagcg tgagtggaat
      781 gttgaaggac cactatgctc agattgttac atcaacgaga tgcaaaaaca ctatgatttg
      841 agtgtcaaac aaaaatgtgt tacctgtgga atagaaaaaa atgtgccaga cctgtgggag
      901 cccagatggc aatgggaaat gaaaggactg ctttgcaagc catgctttga caaaaaggaa
      961 gaagatttta acaaattaaa gaatttttgc agtatttgcg gagcaaaact tggcatcata
     1021 aggtacaatc caaaaaagca gtgggagatt gcaggccaga tctgcagaag ttgttgggac
     1081 atacaaaaag caaaaaggag ttagtagatg caccttttta aaaaattcaa gtgtgaaacg
     1141 tgtgagaaaa aattcaaaca acaagaggag ctaatgcagc acatccaagt tgtgcatggg
     1201 aaagatacgc cttatgactg caagcaatgc aacgagtctt tttcaagcat ggaacagatg
     1261 agaactcatc tgcagaaata tcattcgtat aaaggaacca gagacatcta gacagctttt
     1321 actgttttga ctactggtgg tcttagtttg ttaaagaccc tgaaaccaca aatgcatttt
     1381 atttcaggca atctgcttag ctcagactgg ctaactgggg ttccgcatct tagacatgaa
     1441 taaactacgt taaaagtcgg agcttgtact ttttcttcag caactacggg tgtatcttca
     1501 gatgccatat ttttgattaa aatagtctgt aatttaaggc taacagaatt atttcagtgt
     1561 aagctcgatg taaacatcgt ttggaatttt aattcgcata agctgtctta ttgctctgtc
     1621 atcagcacta agatctataa cacgtctgtg tatcctcatt tcccattttt catatgtgtt
     1681 tgttccttgt ccacatgggg atttgcgtgt aacaacgttt agtcttttta ctggaagtgg
     1741 tgttgggcct ttaactttga cgccagtctt ttctcctatt cctttgattt cactacatac
     1801 accatctaat ctgggcagac tggtacttgt taattttatt ctggccagtt gtgtcatcca
     1861 gaccactagg cggtgtattt ttcagtaata tctttgacaa cgcctgcagc aattgtagca
     1921 cccatatctc ggagtgcgaa tcttcctaat tctggaaagt cttggaaggt ttcaattggc
     1981 agcggtctaa ctggtctgat tcttacgatt gcagaatcac ctgcttttag aaactttgga
     2041 tccttttctt cagcagcacc agtctgtgga tttatttttg cttcaaatgc aacaatggtt
     2101 gctgcaactt gtgctgtgtg tgcatgcatt actggagtgt aaccaggtgc aattgcagta
     2161 ggatgatgaa ttacaataat ttgagctctg aattcttttg caacgtttgg aggactgtca
     2221 ggagttccaa ttacgtcgcc tcttttgata tctttcttat ctacacctct caagttgaaa
     2281 ccaatgttgt cacctgcttc tgcagattcc atttgggtgt gatgggtttc aattgtttta
     2341 atttctccag gtactcctgc tggcatgaca atgatcttca ttcctgcttt tacaactcct
     2401 gtttcaacac gtcctactgg aactgtacct acaccagtga ttgtataaac atcctgaatt
     2461 ggaattctta atggtttgcc agttggtttt tctggaagtt taaagtcatc aaat
//
LOCUS       DATIDL010000072         4292 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrosopumilaceae archaeon isolate SMAG_U16342
            k141_2512666, whole genome shotgun sequence.
ACCESSION   DATIDL010000072 DATIDL010000000
VERSION     DATIDL010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935836
            Sequence Read Archive: SRR25158326
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosopumilaceae archaeon (soil metagenome)
  ORGANISM  Nitrosopumilaceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrosopumilales;
            Nitrosopumilaceae.
REFERENCE   1  (bases 1 to 4292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4292)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/04/2024 05:09:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,520
            CDSs (total)                 :: 1,474
            Genes (coding)               :: 1,474
            CDSs (with protein)          :: 1,474
            Genes (RNA)                  :: 46
            rRNAs                        :: 1 (5S)
            complete rRNAs               :: 1 (5S)
            tRNAs                        :: 43
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4292
                     /organism="Nitrosopumilaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2512666"
                     /isolate="SMAG_U16342"
                     /isolation_source="soil"
                     /db_xref="taxon:2202732"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..259
                     /locus_tag="VLB45_03160"
     CDS             <1..259
                     /locus_tag="VLB45_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007401381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HSD04734.1"
                     /translation="EKQVLDAGAIFVAIGHEPNTKLFKGQIELDEQGYIILKNHTKTN
                     IDGVFAAGDVHDHRYRQAITAAGFGCMAGIDVDKYLAENKK"
     gene            complement(261..1259)
                     /locus_tag="VLB45_03165"
     CDS             complement(261..1259)
                     /locus_tag="VLB45_03165"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HSD04735.1"
                     /translation="MATKVVSDTETLKKIGNTPLVKLNSLSSDKVTHYAKLESHNPFG
                     SVKDRAAYWMIKDGEERGILKKGESIIIEPTSGNTGIALTGIANLLGYKVEIVIPEKA
                     SEETKKIIDSLGAKLFQTSDDLCPKVGAGTDQSIALARAIASARPDIYYMPNQYENEA
                     NFMGHYKGTGPEIWKQTEGKVTHFLTGVGTGGTITGISAYLKEQNPKLKTIAVQPQQN
                     HLIQGLRNFVESDKPVLFKKRENLVDDWMTITNEEAFAAVIKLFEKEKLLVGPSSGAV
                     FAAMEQLGIDQGCIVGIFADDGRKFKSLYAQQKVFTEAQFDKALKEAVHLSQASWV"
     gene            complement(1264..1470)
                     /locus_tag="VLB45_03170"
     CDS             complement(1264..1470)
                     /locus_tag="VLB45_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HSD04736.1"
                     /translation="MRGMFCPEPVFRTKIEMERMTVGNILKVIADDPASEEDISRWVN
                     RNGHQLLEVKKSDKDLEFTIKKVK"
     gene            complement(1575..2495)
                     /locus_tag="VLB45_03175"
     CDS             complement(1575..2495)
                     /locus_tag="VLB45_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP-associated domain-containing protein"
                     /protein_id="HSD04737.1"
                     /translation="MNLLKAELHCHNHFSNFHLGLNETPYDCGITIAEQLEQAYKLGL
                     DVMFVTNHNTLDGYSALLEYQENHQRLKSVHVYPAEEITTNTGIHVIAYGISETIRPG
                     QTIDEIFDKIRSQGAVSCAPHPFGLSNGLREKAIMCDLIEIFNSNNVDRYSNIRAAHF
                     AKSHHMIEIAGSDSHVISTLGRCIDNIDSENTLDDILYAMRKGKISVASTGYITSKEM
                     IEHAKYKISNSKEYIIKYFRQNHPHLTGLCNFLIQTFESNPNSILWKAIYKIGVHLST
                     RLSNKVNFKNYDYTILYERNLRAILPMILK"
     gene            complement(2544..3275)
                     /gene="cofE"
                     /locus_tag="VLB45_03180"
     CDS             complement(2544..3275)
                     /gene="cofE"
                     /locus_tag="VLB45_03180"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010191255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043773 - coenzyme F420-0 gamma-glutamyl
                     ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HSD04738.1"
                     /translation="MTIQIIPVRIDKEIEEGENISDLILLKSEIKDGDILVVTQKIIS
                     KQEGNIVNLSKIKPSLLATGIASAYGKDPRVVQLVLDQTKRIVRMNNGIIISETSHGL
                     VCANAGVDESNVKDGYVTLLPKNADESASKLQQEILKKTGKNIPVIISDTFGRPFREG
                     QTNCAIGMSGIAAILDYVGTKDTFGKTLHVTALAIADEICSATELVMAKSLQTPVAIV
                     RNYKFKNSKGSVNDLIRKRSTDLFR"
     gene            complement(3317..3967)
                     /gene="serB"
                     /locus_tag="VLB45_03185"
     CDS             complement(3317..3967)
                     /gene="serB"
                     /locus_tag="VLB45_03185"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007401371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HSD04739.1"
                     /translation="MLAIFDVEGVLLDAEYLPILAEKINKQDEIWEITRKGIKGVINW
                     EEGLRTRVNTLRGISYETCKEVADSLPIMTGAKEACSALKAAGWKLLAISGGFTIMTD
                     RLKKELGLDHVFSNDLVFNDGKLDGVKIHVDSDKSKSAVIKINEWKEKKKDIVVVVDG
                     ANDIKLFDICGLGVAFRAQDVVKDLATVTLEEKDLSLLIGIINKHYGSKLQLQTVA"
     gene            4070..>4292
                     /locus_tag="VLB45_03190"
     CDS             4070..>4292
                     /locus_tag="VLB45_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007401369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04740.1"
                     /translation="MGELPHKYDVPVKVASKAHKLPDKVKDELKLSGMMDEKGKLKLK
                     GVSPKMLKRMKEEAVDCPVLKEEVGFIQCF"
BASE COUNT         1182 a          848 c          749 g         1513 t
ORIGIN      
        1 cgaaaaacag gtcttggatg ctggagcaat ttttgtcgcc ataggacatg agccaaatac
       61 taaactgttt aaaggacaaa tcgagctaga tgaacaaggg tacatcattc taaagaatca
      121 cacaaaaaca aacatagatg gagtatttgc tgccggcgat gttcacgatc acaggtacag
      181 acaagcaatc actgctgcag gttttggctg catggcagga attgatgtag ataaatatct
      241 tgcagaaaat aagaaataac ctaaacccag gatgcctggg acagatgaac tgcttctttt
      301 agtgccttgt caaattgcgc ctcagtgaat actttttgtt gtgcataaag gctcttgaat
      361 ttcctaccat catctgcaaa tattccaact atgcagcctt gatctatgcc aagttgttcc
      421 attgctgcaa acactgcacc tgatgatggg cctaccaaga gtttttcttt ttcaaacagt
      481 tttatcactg ctgcaaacgc ttcctcattt gtaatagtca tccagtcatc aaccagattc
      541 tcacgctttt tgaataaaac tggcttgtca gattctacaa aattgcgcag tccttgaatc
      601 agatggtttt gctgaggctg gactgctatt gttttgagtt ttggattttg ttctttaaga
      661 taggcagaaa ttcccgtaat tgtcccacca gttcctacac ctgttagaaa gtgagtcact
      721 ttgccttcag tctgtttcca tatttcagga ccagtgccct tgtaatgtcc cataaagttt
      781 gcttcattct catactggtt tggcatatag taaatgtcag gtcttgcaga tgcaattgcc
      841 cttgcaagtg ctatgctttg gtctgtacct gcaccaactt taggacacag gtcatcactt
      901 gtttgaaaca gcttggctcc aagagaatct attatcttct ttgtttcctc acttgctttt
      961 tcaggaatga caatttctac cttgtatccc aacagattgg caattccagt taatgcgatt
     1021 ccggtattac cagaggtggg ctctatgatt atactctctc ctttttttag tatgccgcgt
     1081 tcctctccgt cttttatcat ccaataagca gctctgtctt ttacagaacc aaacggattg
     1141 tgtgattcta gctttgcata atgagtaacc ttatcagaag aaagagaatt taattttaca
     1201 agaggcgtat tcccaatttt ctttagggtt tctgtatcgc ttacaacctt tgttgccact
     1261 tgattatttc acctttttaa tggtgaattc taaatccttg tcagattttt taacttctaa
     1321 aagctggtga ccatttctgt tgacccatct tgaaatatct tcttctgatg ctggatcgtc
     1381 tgcaataacc tttagaatgt ttcctacagt cattctttcc atttctattt ttgttctaaa
     1441 tactggttcg gggcagaaca ttcctcgcac atcaattgtt ttagctggac ctaattctga
     1501 aatatcaatt caccttacaa tttcatttca aaccaacaat attaaaatct attcttgtgt
     1561 gcaacaatct tttgttactt gaggatcatt ggcaagattg ctctcaagtt acgttcatat
     1621 aaaattgtgt aatcatagtt tttgaaattt actttgttag aaagccttgt ggataagtgt
     1681 actccaattt tataaattgc cttccataaa atgctgtttg gatttgattc aaacgtttga
     1741 ataagaaaat tgcacagtcc agtcaagtgg ggatggtttt gtctaaagta ttttattatg
     1801 tattcttttg aatttgagat tttgtatttt gcgtgttcta tcatttcttt acttgtgata
     1861 tatccagtgc ttgcaacaga aatctttcct ttcctcattg catacagtat gtcatcaagt
     1921 gtgttttcag aatcaatgtt gtctatgcat ctaccaagtg tagagataac atgtgaatcg
     1981 ctgcctgcaa tctctatcat gtggtgagat tttgcaaagt gtgctgccct gatgtttgaa
     2041 tatctatcaa cattattact attaaaaatc tcaatcaagt cacacattat tgctttttca
     2101 cgcagtccat tgcttaaacc aaaaggatgt ggtgcacatg aaactgcacc ttgtgatctt
     2161 attttatcaa atatctcgtc aatggtttga ccaggcctta ttgtttcaga tattccatat
     2221 gcaatgacat gtatgccagt attagtagta atttcttctg cagggtacac atgcacgctt
     2281 tttagtcgtt ggtgattttc ttgatattca agcaatgcag aatatccatc aagcgtgtta
     2341 tgatttgtta caaacatgac atccaagcca agtttgtagg cttgttccag ttgttctgca
     2401 atagttattc cgcagtcata cggtgtttcg ttcaagccga gatgaaaatt agaaaaatgg
     2461 ttgtgacagt gtagttcagc ttttaaaagg ttcaacggtt attcgtgttg cttagcttat
     2521 catataatcc ttattttata taatcatcgg aatagatctg tcgatctttt tctgatgaga
     2581 tcatttactg aaccttttga atttttgaat ttataatttc taactatcgc tacaggcgtt
     2641 tgaagcgatt ttgccataac aagctcagtg gcagagcaaa tttcgtctgc aattgcaagt
     2701 gcagtaacat gtagagtttt gccaaaagtg tctttggttc caacgtaatc aagtatggca
     2761 gcaatccctg acattccaat ggcacaattt gtttgtcctt ctctaaatgg cctaccaaaa
     2821 gtatctgaaa ttataactgg tatgtttttt ccagtttttt ttaatatctc ctgttgtagc
     2881 ttgcttgcag attcatctgc attttttggc aacagtgtga catagccatc ttttacgttg
     2941 ctttcatcca cacctgcatt tgcacaaaca agtccatgag atgtttcaga tattattatt
     3001 ccattgttca tccttacaat gcgttttgtt tgatcaagca ctagctgaac tactcgtgga
     3061 tcttttccat atgcacttgc aataccggtt gctaaaagcg atggttttat tttagataga
     3121 tttacaatgt ttccttcttg tttggaaata attttttgag taactacgag aatgtctccg
     3181 tcctttatct cagattttaa gagtataagg tcagatatat tttcgccttc ctctatctcc
     3241 ttatcaattc taacaggaat tatttggatt gtcatttagg aattctgtat tgtcattgtt
     3301 taaaataata ccgaatctat gcaacggttt gaagttgaag ctttgaacca tagtgcttgt
     3361 tgattattcc aattaatagt gagagatctt tctcttcaag tgttactgta gctaggtctt
     3421 ttacaacatc ctgtgctcta aacgccacac ctagtccaca gatatcaaac agtttgatgt
     3481 catttgcacc atcaactact actacgatgt cttttttctt ttctttccat tcgtttattt
     3541 ttatcactgc agattttgat ttgtcagaat ctacatgtat ttttacgcca tctagtttcc
     3601 catcattgaa gacaagatcg tttgaaaaca catgatctaa tcccaactcc tttttcagtc
     3661 tgtcagtcat tatggtaaat ccaccagata ttgctaataa tttccagcct gctgctttta
     3721 gagcgctgca tgcctcttta gcgccagtca tgattggcaa agaatctgca acttctttgc
     3781 atgtctcata gcttattcct cttagcgtgt ttactctggt tcgcaatcct tcttcccagt
     3841 ttatcacgcc tttgattcct tttctggtaa tttcccagat ttcgtcttgt ttgtttattt
     3901 tttcagcaag aatcggaaga tattcagcgt caagtaagac tccctcgaca tcaaaaattg
     3961 ctagcattag tttctgattt tgaaacagaa aatctgatta tattaatctt cagagatcag
     4021 atcaaacaaa aaccactagc aatatattct acaacactaa atccccatca tgggcgagct
     4081 tccacacaaa tacgatgttc cagtaaaagt tgcatcaaaa gcacacaagc ttccagacaa
     4141 ggtaaaagac gaactaaaac tgtcaggaat gatggatgaa aagggcaagc tcaaattgaa
     4201 aggcgtcagt ccaaaaatgc taaagcgaat gaaagaggaa gccgttgatt gtccagtgct
     4261 taaagaagaa gtgggcttca tacagtgctt tg
//
LOCUS       DATIDL010000073         2257 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrosopumilaceae archaeon isolate SMAG_U16342
            k141_1907904, whole genome shotgun sequence.
ACCESSION   DATIDL010000073 DATIDL010000000
VERSION     DATIDL010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935836
            Sequence Read Archive: SRR25158326
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosopumilaceae archaeon (soil metagenome)
  ORGANISM  Nitrosopumilaceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrosopumilales;
            Nitrosopumilaceae.
REFERENCE   1  (bases 1 to 2257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/04/2024 05:09:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,520
            CDSs (total)                 :: 1,474
            Genes (coding)               :: 1,474
            CDSs (with protein)          :: 1,474
            Genes (RNA)                  :: 46
            rRNAs                        :: 1 (5S)
            complete rRNAs               :: 1 (5S)
            tRNAs                        :: 43
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2257
                     /organism="Nitrosopumilaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1907904"
                     /isolate="SMAG_U16342"
                     /isolation_source="soil"
                     /db_xref="taxon:2202732"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..908)
                     /locus_tag="VLB45_03195"
     CDS             complement(<1..908)
                     /locus_tag="VLB45_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016939408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HSD04741.1"
                     /translation="MQQNILGAVDEVTTTMPPALLVSATYDSQKKVAVLKFYEPVSQK
                     VLLWTDNTNHKPYCYSKLDPEELKFLSDRKDVLKLEKIERQDLLKDKMISVTKIIVAD
                     PLAIGGTQTDKSIRNIIETWESDIKYYENYLYDRSLIVGRYYRIVEGELESYSYDIPD
                     EVQLALKGLLWDKVTNIGMINPKEFQDHISDWANLLNQPIPKIRRVSFDIEVESEVGR
                     IPDPKVAEKKITAMGFSGSDGMKQVFVLKRKDKKLGTSELASDIKITFYEEDQEKEMI
                     KDGLKVLTDYPFVITFNGDEFDMPYV"
     gene            complement(918..1454)
                     /locus_tag="VLB45_03200"
     CDS             complement(918..1454)
                     /locus_tag="VLB45_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008299515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase family protein"
                     /protein_id="HSD04742.1"
                     /translation="MKKSFERPTWDEYFMLQAELAKLRSNCITRQVGAVIVREHRQIA
                     TGYNGTPPGVTNCFEGGCKRCLLRAEGKIASGEGLDRCICNHAEANAIMHCAILGVGS
                     GTKDTTLYTTFVTCLECSKMAITIGIKRIVCLGSYPETDFDLLKEAGLEIVMLDKKKI
                     EYWIRVLLEDPAANLIPK"
     gene            1583..>2257
                     /locus_tag="VLB45_03205"
     CDS             1583..>2257
                     /locus_tag="VLB45_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007550724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HSD04743.1"
                     /translation="MSQQYIVHKLIKKNTVEYREYQENLAKQAISENCLVVLPTGLGK
                     TTVALQVMAEFLSKKTGGVLFLAPTRVLAHQHYEFLKNNLLIDDIVLITGEDLVSKRK
                     KLWMNSVVCATPEITKNDLDRQLVSPDQFVLLIFDEAHRTIGDYAYSGIAERFKNTNI
                     RIIGMTATLPSEREKAAEILDTLKITSIAQRTEDSPDVKPYVQQTDTQWVTVELPPEM
                     KEIQTLV"
BASE COUNT          653 a          475 c          421 g          708 t
ORIGIN      
        1 tacacatatg gcatatcaaa ttcatccccg ttgaatgtga tcacaaatgg ataatctgtc
       61 aaaactttca ggccgtcttt tatcatttcc ttttcttggt cttcctcgta gaatgtgatt
      121 ttgatgtctg acgcaagctc acttgtgcct aactttttgt ctttgcgctt tagcacaaag
      181 acttgtttca ttccatcact gccagaaaat cccatggctg taatcttttt ttcagccact
      241 tttgggtcag gaatccttcc tacctcagat tctacctcaa tatcaaagct aactcgtctg
      301 atttttggga tgggttgatt taacaggttt gcccagtcag agatgtggtc ttgaaattcc
      361 ttaggattga tcattccaat gtttgtaacc ttatcccaca gcaacccctt tagggcaagc
      421 tggacttcat ctggaatatc atagctatag gattctagtt ctccttcaac tattctatag
      481 tatcttccta caataagaga acgatcgtaa agataatttt cataatactt gatgtcagat
      541 tcccaagttt cgattatgtt tcttatgctc ttgtctgttt gtgttccacc aattgccagc
      601 ggatctgcga ctatgatttt tgttactgaa atcattttat cttttaacag gtcttgtctt
      661 tcaatttttt ctagctttag tacatctttt ctgtctgaca aaaactttag ttcttcagga
      721 tcaagctttg aatagcaata tggtttgtga ttagtgttgt cagtccacag taaaactttt
      781 tgtgaaaccg gttcatagaa ttttaaaacc gcaacttttt tctgactgtc atatgttgca
      841 gagactagca atgctggcgg cattgtagtt gttacttcat ctactgcacc caagatattt
      901 tgttgcatgc actcacctta ttttggaatc agatttgcgg ctggatcctc aagcagcacc
      961 ctaatccagt attcaatctt ttttttgtcc agcatgacaa tttctaatcc agcttctttt
     1021 agtaaatcaa aatcagtctc agggtatgaa ccaaggcaga ctatgcgttt tatccctatg
     1081 gtgattgcca ttttactgca ttctaggcat gttacaaacg tggtgtaaag tgttgtgtct
     1141 ttggtaccag aacccacacc caaaatggcg cagtgcatta ttgcgtttgc ctcggcatga
     1201 ttgcagatac acctgtcaag gccctcgcca gaggctatct ttccttctgc gcgcaaaagg
     1261 caccttttgc atccaccctc aaagcagttt gtcacaccag gaggtgtgcc attatacccc
     1321 gttgcaattt gtcggtgttc acgtacaatt actgcaccta cctgtcgcgt tatacaatta
     1381 gaacgaagtt tggccaattc agcctgtaac ataaagtact catcccatgt gggacgctca
     1441 aaagattttt tcatactgga tttgatattt acctcaatat aagattgaac tgacattatc
     1501 caagtcttga tcagccatta gcttctgata tcagattcat cgaccacgaa actttttact
     1561 tgtccagata aagagatctt gcttgtcaca acagtacata gtgcacaaat taattaaaaa
     1621 aaacactgta gaatataggg aatatcagga aaaccttgca aaacaggcaa taagtgaaaa
     1681 ttgcctggta gtattgccta caggacttgg caaaacaaca gtcgcattac aagtgatggc
     1741 agaattctta tcaaaaaaaa ctggcggagt cttatttctt gcgccaacaa gggttctggc
     1801 acaccagcat tatgagtttt tgaaaaataa tttgctcata gatgacatag tactgattac
     1861 tggcgaggat ctcgtatcaa aaagaaaaaa actatggatg aatagcgtcg tttgcgccac
     1921 accggaaatt acaaaaaatg atcttgacag gcagttggta tcaccggatc aatttgtttt
     1981 gttgatcttc gatgaggcac atagaaccat aggagattac gcatattcag gaattgcaga
     2041 acgcttcaaa aatacaaaca ttcgcataat tggaatgact gcaactcttc caagtgagag
     2101 agagaaagct gcagagatac ttgatactct gaaaatcacc agcattgcac aaaggacaga
     2161 agacagtccg gatgtgaaac catacgtaca acaaaccgat acgcagtggg ttacagttga
     2221 gcttccaccg gaaatgaaag agattcagac actggta
//
LOCUS       DATIDL010000074         2849 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrosopumilaceae archaeon isolate SMAG_U16342
            k141_2109229, whole genome shotgun sequence.
ACCESSION   DATIDL010000074 DATIDL010000000
VERSION     DATIDL010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935836
            Sequence Read Archive: SRR25158326
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosopumilaceae archaeon (soil metagenome)
  ORGANISM  Nitrosopumilaceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrosopumilales;
            Nitrosopumilaceae.
REFERENCE   1  (bases 1 to 2849)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2849)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/04/2024 05:09:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,520
            CDSs (total)                 :: 1,474
            Genes (coding)               :: 1,474
            CDSs (with protein)          :: 1,474
            Genes (RNA)                  :: 46
            rRNAs                        :: 1 (5S)
            complete rRNAs               :: 1 (5S)
            tRNAs                        :: 43
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2849
                     /organism="Nitrosopumilaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2109229"
                     /isolate="SMAG_U16342"
                     /isolation_source="soil"
                     /db_xref="taxon:2202732"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..537)
                     /locus_tag="VLB45_03210"
     CDS             complement(<1..537)
                     /locus_tag="VLB45_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007549807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HSD04744.1"
                     /translation="MSATETLRQDHIQIKRLKKIIIKCYTKLYAGENIPLSDIEQITI
                     IMAEFLDAIHYSREEDAYFACVAGYDSLKKEIRAFMIEHEFGRRIAKSVMSHLKQWKE
                     GKDSREPVARFLRTYAIYLNDHITKEEQFFDIAEKQILSPEEEKEMYEEFRAVNAVIT
                     KLDEMVKSINSLEEKSWMK"
     gene            complement(557..1393)
                     /locus_tag="VLB45_03215"
     CDS             complement(557..1393)
                     /locus_tag="VLB45_03215"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HSD04745.1"
                     /translation="MDLVFSDIHADIGALETILEIATSDDFIKRYGSFDRIINLGDLL
                     ERGTNPKQVLDKMNTLSKNYKLLSVLGNHDEAFLYKRPISSGSIESWAAHNTLKDDDL
                     SFFPQNKDGSFGQQQYVDTKNNLVCVHGGPLDPKKITPKNAYDEETWMYQKTWQRLSE
                     EDFEFFSYSGYHYKPTSAFEEGKNHLDNFVILCGHQHKETILVQNDHIKEIFSSLKPT
                     TEKASKFVLEKREIEIEKSNNYIIRLGLGGPSGYYGVGSPNPHFGIIQYNPKKVALFT
                     VK"
     gene            complement(1417..2631)
                     /locus_tag="VLB45_03220"
     CDS             complement(1417..2631)
                     /locus_tag="VLB45_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010191996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CFI-box-CTERM domain-containing protein"
                     /protein_id="HSD04746.1"
                     /translation="MRNLLALAFFSLLLLPLNLAWAEYDSSHLRVPEISMTPTSGPPG
                     TEITITVSNFPDTSNEVYPYPDFYVYLPFADAIGDSNVPGNCDGESCFPIYTYQDAQK
                     GNFADKIITFKLFSTNNPKPVYLDNKLHTVCDVMVNNKAQQSFTDICSKQNQPMGDYE
                     IKFAWGTITGEYDIVETMTFTVTEGNVAPEPESLRLDQLLMDLYANGTITEEEFEEEL
                     RLLGYTEAEIRQAKGILGKLPHQENADSPTPTPVENTENDDVTPIEDSDEKPDTTTPQ
                     NGCLIATAAFGSELAPQVQILRELRDNTLLKTSSGSSFMTAFNSFYYSFSPTVADWER
                     QNPFFKEAVKLTITPLISTLSILNHVNIDSEAEMIGYGIGIILLNIGMYFVAPAIVVS
                     QLNSKLKHKVRN"
BASE COUNT          707 a          515 c          583 g         1044 t
ORIGIN      
        1 tttcatccat gatttttctt ctagagaatt tattgatttt accatttcat ctagcttggt
       61 gatgacagcg ttgactgctc taaactcttc atacatttct ttttcttctt caggtgacag
      121 gatctgtttt tctgcaatgt caaaaaactg ctcctctttt gtgatgtgat cgttaaggta
      181 aatagcataa gtcctgagaa atctggcaac tggttccctt gaatccttgc cttccttcca
      241 ttgctttaga tgagacatga cgctttttgc tattcttctg ccaaactcgt gctctatcat
      301 aaatgcgcga atctcttttt ttagagaatc atagcctgca acacacgcaa agtatgcatc
      361 ctcttccctg gaatagtgta ttgcatctag aaattctgcc atgattattg tgatttgttc
      421 aatgtcagaa agaggtatgt tttctccagc gtacagtttt gtataacact tgatgattat
      481 tttcttaagt cgttttatct ggatgtggtc ttgccgaagt gtttctgttg cgctcatcag
      541 ttttcagata cctgcattat tttaccgtga ataaagctac cttctttgga ttgtactgta
      601 ttattccaaa gtgcgggttt ggggaaccga ctccatagta accgcttgga ccgccaaggc
      661 caagcctgat tatgtagttg tttgattttt caatctcgat ttctcttttt tcaagcacaa
      721 acttggatgc cttttctgtt gttggtttta gcgaagaaaa gatttctttg atgtgatcat
      781 tttgcactag tatagtttct ttgtgctgat ggccacataa gattacaaaa ttgtctagat
      841 ggttttttcc ttcttcaaac gcggatgttg gcttgtagtg atatccggaa tagctgaaaa
      901 actcaaaatc ttcttcagat aatctctgcc aagttttttg gtacatccag gtttcttcat
      961 catatgcatt ttttggtgtg attttttttg gatctaatgg tcctccatga acgcatacca
     1021 gattgttttt tgtgtcgaca tactgctgct ggccaaagct gccatccttg ttttgtggga
     1081 aaaatgagag gtcgtcatct tttagggtgt tgtgtgcagc ccaactttct atggagccgc
     1141 tgctgattgg tcttttgtac aaaaatgcct catcatggtt tcctagtact gaaagtagct
     1201 tgtaattttt tgaaagtgta ttcattttgt caagtacttg ttttgggttt gttcctcgct
     1261 caagcaaatc tccaagattt attatcctgt caaaactgcc atatcttttg ataaagtcat
     1321 cacttgtggc tatttctaaa atagtctcta gagcaccaat gtctgcatgt atgtcagaaa
     1381 aaactagatc caattgtaca ataatttgtc tgaagcttaa tttctaactt tatgttttag
     1441 tttagagttt agctgggaaa ctactattgc aggcgcaaca aagtacattc caatgtttag
     1501 cagtatgata ccaattccat agccaatcat ttcagcttct gaatcaatgt tgacatggtt
     1561 tagaatcgat agtgttgaga tcaatggtgt gatggttagc tttactgcct ctttgaagaa
     1621 tggattttgc ctttcccaat cggctactgt aggactaaag gaatagtaga atgaattgaa
     1681 cgcggtcata aaagaggaac cagaggatgt cttgagtaac gtgttgtctc gcaattccct
     1741 gagaatctgg acttggggtg ccaattctga tccaaatgct gcagtagcta taaggcaacc
     1801 attttgtggt gtagtggtgt ctggtttttc gtcagaatct tctatgggtg taacgtcatc
     1861 attttctgtg ttttctactg gagttggtgt tggactgtca gcattttctt ggtgtggaag
     1921 ctttcccaaa attcctttgg cttgacgaat ctctgcctct gtatatccaa gtagtcttag
     1981 ctcttcttca aattcttctt ctgtgatcgt accatttgcg tagagatcca tgagaagttg
     2041 atcaaggcgt agactttcag gctctggagc aacgtttccc tctgttactg taaatgtcat
     2101 tgtttctaca atatcatatt ctcctgttat ggttccccac gcaaatttta tttcataatc
     2161 tcccattggc tggttttgtt ttgagcaaat gtcggtaaag ctttgctgtg ccttgttgtt
     2221 taccattaca tcacacactg tgtgtagttt gttatcaaga tagactggct ttgggttgtt
     2281 tgtgctgaaa agcttgaatg tgattatttt atctgcaaag tttcctttct gtgcatcttg
     2341 atatgtgtaa attggaaaac aagattctcc atcgcagttg cctggcacgt tgctgtctcc
     2401 tattgcatct gcaaatggca agtagacgta aaagtctgga tatggataca cttcatttga
     2461 tgtgtcagga aagtttgaga ctgtaattgt aatttcagtt ccgggtggtc cggaagttgg
     2521 tgtcattgat atttctggca ctcgcagatg gcttgaatca tattctgccc aagctaaatt
     2581 tagtggtaag agtaaaagtg agaaaaatgc taaggccagt agatttctca aatctgggtt
     2641 gatctcatgt gcataattat taagaattgc tactagatca ttagtaagtt tgaggatttt
     2701 gggtttagat tgattcgctt tttgataaag tgagaattag gcacatgcac gaacaaaaaa
     2761 gcgcaaataa gagcactttt tgggtgtttt ttgggtcgaa tttcaactga ttttctgatc
     2821 gcaaaataac tttcattttt acgattctg
//
LOCUS       DATIDL010000075         6451 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrosopumilaceae archaeon isolate SMAG_U16342
            k141_699074, whole genome shotgun sequence.
ACCESSION   DATIDL010000075 DATIDL010000000
VERSION     DATIDL010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935836
            Sequence Read Archive: SRR25158326
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosopumilaceae archaeon (soil metagenome)
  ORGANISM  Nitrosopumilaceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrosopumilales;
            Nitrosopumilaceae.
REFERENCE   1  (bases 1 to 6451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/04/2024 05:09:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,520
            CDSs (total)                 :: 1,474
            Genes (coding)               :: 1,474
            CDSs (with protein)          :: 1,474
            Genes (RNA)                  :: 46
            rRNAs                        :: 1 (5S)
            complete rRNAs               :: 1 (5S)
            tRNAs                        :: 43
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6451
                     /organism="Nitrosopumilaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_699074"
                     /isolate="SMAG_U16342"
                     /isolation_source="soil"
                     /db_xref="taxon:2202732"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..714)
                     /locus_tag="VLB45_03225"
     CDS             complement(<1..714)
                     /locus_tag="VLB45_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HSD04747.1"
                     /translation="MSLTQQEHGFLEKKIGRKPNPLELQIVAAEWSEHCSYKSSKKFL
                     KLLPKEGTRVISGPGYDSGVLDVGDDYVVTVHIESHNHPSAVEPYGGAATGVGGVIRD
                     ILSAGTRPIAILDGLRFGNIEKDPHAKWLFKNAVRGIADYGNCLGIPTIGGEIEFDEC
                     FTNYALVDVAAIGFGKKNKLIRNRADKDDFVVLVGGSTGRDGIGGSQFASDKLESENR
                     SAVQIPDPFIEKLVIEAVLE"
     gene            complement(711..1391)
                     /gene="purQ"
                     /locus_tag="VLB45_03230"
     CDS             complement(711..1391)
                     /gene="purQ"
                     /locus_tag="VLB45_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HSD04748.1"
                     /translation="MKVGIVVFPGSNCDRDMHHVLTDVFKLHTELLWHEQELPKNLDA
                     IILPGGFSYGDRLRAGVIAAHSPVISGIKKMAEKGMPILGVCNGFQILVESGLLPGAL
                     LKNTSLNFMCKWTELIVKNNKTPFTNHFKLNQRIPIPIANGEGRYYADKETLRTMKKN
                     NQIVFTYCEEVNGSTNNIAGVCNKQGNVVGMMPHPERAAESAINPKNSKPASMIFESL
                     LTSIGVVQ"
     gene            1469..1912
                     /locus_tag="VLB45_03235"
     CDS             1469..1912
                     /locus_tag="VLB45_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HSD04749.1"
                     /translation="MTHVRDIMIRNVITIEHDKTAKDVALLMAERQVSSLIVIKDETP
                     IGLVTERDLARKISTTDRKSSDVPLSDIMSLKFRWVEPMTPVEDAIQKMINNNIRRLL
                     VLENGKLVGIITETDLAKHLRSKLLIDGALDVTALKRKVVKSSKK"
     gene            complement(1917..2177)
                     /locus_tag="VLB45_03240"
     CDS             complement(1917..2177)
                     /locus_tag="VLB45_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HSD04750.1"
                     /translation="MPSFKVSVTIENKPEISDPEGDTILHDLILKGNHSSVKKIRSAK
                     MLKFEVDAKNKKLAEKIVLDLCNEFRIYNPLVSRVSVESTDI"
     gene            2262..2336
                     /locus_tag="VLB45_03245"
     tRNA            2262..2336
                     /locus_tag="VLB45_03245"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2297..2299,aa:Thr,seq:tgt)
     gene            2387..2626
                     /locus_tag="VLB45_03250"
     CDS             2387..2626
                     /locus_tag="VLB45_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HSD04751.1"
                     /translation="MQMGYVLLNCDLGSEEYILEELKRIPQVKKAYITFGSYDIIAEI
                     NAQTQEDFDKAISLRIRSLSRVISTMTLKVVNPEG"
     gene            2655..2942
                     /locus_tag="VLB45_03255"
     CDS             2655..2942
                     /locus_tag="VLB45_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007551007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04752.1"
                     /translation="MTNIEYTGTVWAINHGLPEPLLNHCVKKLIDHGIKKEDIKITDA
                     PENVKIGTIVVDIWPYHLEIGRVRTVRNESFISGSVMVIELKADADGNYID"
     gene            2995..3504
                     /locus_tag="VLB45_03260"
     CDS             2995..3504
                     /locus_tag="VLB45_03260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04753.1"
                     /translation="MKKKSLKSSKKGTYVSIMAGAIIIGIVIIISYYTDQAKISGQRF
                     GNDLEQIQSDLKKIASEYESNFTMWEEGRLTKQEILEISDNYLIELEKIISRYGELTP
                     PPSFAPSLDLFKRSVESQLESDRMLKEWIQTGDNAAKIKSDELLQQAFEYETSALASF
                     NRAKISNTQ"
     gene            3537..4295
                     /locus_tag="VLB45_03265"
     CDS             3537..4295
                     /locus_tag="VLB45_03265"
                     /inference="COORDINATES: protein motif:HMM:NF038353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxLYD domain-containing protein"
                     /protein_id="HSD04754.1"
                     /translation="MKLLLTGVLSLILIFPNAWAQVSIVNDQNYVGNEGVFHIVGEIE
                     NSSTVPLNQITVYATFYSLDGKSLGTKYTNSILETIMPDKKGPFDLAINESWISELNH
                     YSLGVKYVPTDHKTESLEILSSHARLDIVDNFIISGTIVNHDEKTANTIVVIATFYDK
                     DGKVVATAKTYTEPDYLKSGGEVPFLLSVSDKLQSKKISDYYLTVESEEYTAVPEFPF
                     GSSIVLISSVIAYLLFTRKPRIVITGLSRAMNLK"
     gene            complement(4258..4716)
                     /locus_tag="VLB45_03270"
     CDS             complement(4258..4716)
                     /locus_tag="VLB45_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007551009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04755.1"
                     /translation="MKWKLEREDEFFKIFDENKNIAGYFDPEYGEIRPEHKVYEIIEE
                     MHKRHDKISGGYLMVPMVKFGVFGGDREIDLDLLQTKIDEVKKRVTRWQEFILHKKNS
                     QHKIGVSHTDSDMLSITFPISFTVPVPLEKNQILAELEIILNSLHEKGLL"
     gene            4971..5252
                     /locus_tag="VLB45_03275"
     CDS             4971..5252
                     /locus_tag="VLB45_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04756.1"
                     /translation="MAITPSTKDFIENLINYYVSEAPSYREIAEIYTAEVKNIPDTTF
                     GIILGCVYSGFLQTYTNQKQTPALEDIQEFNQIIKQRASEIKKAILDKS"
     gene            5294..5617
                     /locus_tag="VLB45_03280"
     CDS             5294..5617
                     /locus_tag="VLB45_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04757.1"
                     /translation="MSETVHYKIQQLVDNGQIVQISLVEDVQLEPVSQKQIMLDAINK
                     KLDPELKEQVMPLIEAIMQAQPTIAVKSYAQTSITISMPRRRYEMIGSPRVGEKLEIN
                     VKKIS"
     gene            complement(5614..>6451)
                     /locus_tag="VLB45_03285"
     CDS             complement(5614..>6451)
                     /locus_tag="VLB45_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HSD04758.1"
                     /translation="RSKRIAPGRDDKILTSWNGLMISAFAKGYRISGLNEFLDATQNC
                     ITFIEEKLTKNGKLLRTYKNGQAKLMAYLDDYVYFVNALLDVFEINPDAKYLDLAVKH
                     ADYLIDHFWDESENNFFFTADDHEKLIIRTKNLYDLSLPSGNSVAAYAFLKLYHLTQD
                     KKFLDVSTKIMESLAIMAAENPFGFGQLLNAMYLYLKKPIEITLLNTKDHEIYYALVK
                     KFLPESIFVTIRNKEQLTKLANYQFFAGKQFDKNKTIVFVCKDFTCSLPLDNLSEIEK
                     LI"
BASE COUNT         2172 a         1144 c         1111 g         2024 t
ORIGIN      
        1 ttcaagcaca gcttctatga ctagtttttc aatgaaagga tctggaatct gaaccgctga
       61 acgattttct gattctaatt tatctgaggc aaattgtgaa ccgccaatac catcacgtcc
      121 agtagaacca ccaactaaaa caacaaaatc atccttgtca gccctatttc gaattagttt
      181 gtttttcttt ccaaaaccaa ttgcagcaac atcaacaagt gcataatttg taaaacactc
      241 atcaaattca atttctcccc cgatggttgg aattccaaga cagttcccat agtcagcaat
      301 tccgcgtaca gcatttttga atagccattt tgcatgcgga tctttttcta tatttccaaa
      361 ccttaagcca tcaagtattg caattggtct tgttccagca gataagatat ctcttataac
      421 tccaccgact ccagtagcag ctccaccata cggttcgact gcagaaggat gattatgact
      481 ttcaatatga acagtaacaa cataatcatc acctacatca agcacgccgg aatcataacc
      541 cggtccagaa attacacgcg ttccttcttt tggaagaagt tttagaaatt tttttgagga
      601 tttgtatgaa cagtgttcag accattcggc tgcaactatt tgtagttcaa gcggatttgg
      661 ttttcgacca attttttttt cgagaaaacc atgttcttgt tgagtaagac tcattgtact
      721 acccctatgg aggttagaag agactcaaat atcatagagg caggttttga attctttgga
      781 tttattgctg attctgcagc tctctcagga tgaggcatca tgcctacaac gttaccttgt
      841 ttattgcaga ctcctgcaat gttatttgta gaaccgttta cttcttcaca ataggtaaag
      901 acaatttgat tatttttctt cattgtacgt agtgtttcct tatctgcata gtatcttccc
      961 tcaccatttg caatggggat tggaattctt tgatttagct taaaatgatt agtaaatgga
     1021 gtcttgttat tctttactat aagctctgtc catttgcaca taaaattaag tgaggtattt
     1081 ttaagaagag caccaggcaa aagtccagat tctacaagaa tttgaaagcc gttacaaact
     1141 ccaagaattg gcattccttt ttctgccatt ttttttatac ctgaaataac aggactgtgt
     1201 gcggcaataa ctccagcgcg aagtcggtct ccatatgaaa atcctcctgg cagaattatg
     1261 gcatcaagat tctttggcaa ttcttgttcg tgccataaga gttcagtgtg cagtttaaag
     1321 acatcagtga gtacatggtg catatcccga tcgcagttgc ttccaggaaa aaccacaatg
     1381 cctactttca cacttttctt attttatcag gattattaaa ttgaatcgaa tgaaatcgtt
     1441 tttattcaat ttctttttgg taatagctat gacacatgta cgtgatatca tgattcgtaa
     1501 tgtaatcaca atagaacatg ataaaacagc aaaagacgta gcacttttaa tggcagaaag
     1561 acaggttagc tcgctaatag tcataaaaga tgaaacccca attggactgg ttacggaaag
     1621 agacctggca cgcaaaattt caactacaga tagaaaaagt tctgatgtgc cactttcaga
     1681 tataatgtcc cttaaatttc gctgggtcga accaatgaca cccgtagaag atgccattca
     1741 aaaaatgata aacaacaaca tacgtagact gcttgtttta gaaaacggca agcttgttgg
     1801 cattataact gaaacagact tggcaaaaca cctcaggagc aagcttctca tagatggggc
     1861 gctagatgtc accgccttaa agcgaaaagt tgtaaaaagc agtaaaaaat agtgtctcaa
     1921 atatcggtgc tttcgacaga tacgcgactt accagtgggt tgtatattct aaattcgttg
     1981 cataaatcaa gtacaatttt ttctgcaagt tttttgtttt ttgcatcaac ttcaaacttt
     2041 aacatctttg cagaacgaat tttttttaca gaagaatggt ttccttttaa gataaggtca
     2101 tgcaatatgg tatcaccttc tggatcactt atttctggtt tgttttctat ggtaacgcta
     2161 actttgaaag aaggcatgtg tggtttgttt agttgtaatt aatttaagct ttggtttatt
     2221 tgataacatt ttaaaccatc tagattttcc tcatacatta tgccaatgta gctcagcctg
     2281 gctagagcat ctgacttgta atcagaaggt cgagggtcca aatcccttca ttggctttca
     2341 aaataataga caaagttaag taacaaacta gtgtagaaga aaaatcatgc agatgggtta
     2401 cgtgttgctt aattgtgatc taggttctga agaatacata cttgaggagt taaaacgcat
     2461 tccacaagta aaaaaggcat acataacatt tggctcttat gacataatag cagaaattaa
     2521 tgctcagact caagaggatt ttgacaaagc aatttcactt aggataagaa gcttgtcaag
     2581 agtgataagc acgatgacat taaaagtggt aaatcccgaa ggataattat agaagaaaag
     2641 aaaaacaact caaattgaca aacatagaat ataccggaac cgtctgggca ataaaccacg
     2701 gattaccaga accattactt aatcactgtg tcaaaaaact aatcgatcat ggaataaaaa
     2761 aagaagacat caaaattacg gatgcaccag aaaatgtcaa aattggtaca attgtggtag
     2821 atatttggcc atatcatctt gaaataggac gtgttcgtac agttcgaaac gagtcattta
     2881 tcagtggcag cgttatggtt atagagctta aagcagatgc cgatggaaat tacatagatt
     2941 aggtttacgt catattcaga atagatttat ttcattaata aaaaaactta gaatgtgaag
     3001 aaaaaatctt tgaaatcaag caagaaagga acatatgttt caattatggc gggagccata
     3061 atcatcggaa tagtaattat aattagctat tacacagatc aggcaaaaat ttcaggtcag
     3121 cgatttggta atgatcttga acagatacaa agtgacctaa aaaaaattgc ttcagagtat
     3181 gaatcaaact ttaccatgtg ggaagaagga agattgacaa aacaagaaat cctagagatc
     3241 tcagacaact atcttattga acttgaaaag atcatctcaa gatatggtga attaacaccg
     3301 ccaccgagtt ttgcaccttc attggatctt tttaaacgct ctgtagagtc ccaacttgaa
     3361 agcgacagaa tgctaaaaga atggattcaa acaggagata atgctgccaa gatcaagtca
     3421 gatgagcttt tgcagcaagc gtttgaatat gaaacaagcg cacttgcatc atttaacagg
     3481 gcaaagataa gtaatacaca ataagttctt taaactatga atcagacata tcagtgatga
     3541 aactactctt aactggagtg ctttccttga tcttaatttt cccaaatgct tgggcacagg
     3601 tttctatagt caatgatcag aattatgtag gcaacgaagg tgtttttcac attgttgggg
     3661 aaattgaaaa ttcttccacc gttccattaa atcaaataac agtatatgca acattttatt
     3721 cgttagatgg aaaaagtctt ggcacaaaat atactaattc tattcttgaa acaataatgc
     3781 ctgataaaaa aggacccttc gatttagcga tcaatgaaag ttggatttca gagctcaatc
     3841 attattcttt gggtgtgaaa tatgtaccga ctgatcataa aactgaatca cttgaaatac
     3901 tatcttcaca tgcaagactt gacattgtgg ataatttcat cataagcgga accatagtta
     3961 accatgatga aaaaacagct aataccatag ttgtaatagc aacattttac gataaagacg
     4021 gcaaagttgt cgccaccgcc aagacataca cagagccaga ctatctaaaa tcaggtggag
     4081 aagtaccatt tttactatct gtgtcagaca aattgcaatc aaaaaagatc agcgattatt
     4141 atctaacagt tgaatctgag gaatatactg ccgtaccaga atttccattt ggttcctcta
     4201 ttgtactcat atcatcagta attgcatatt tactgtttac aagaaagcca aggattgtta
     4261 taacaggcct ttctcgtgca atgaatttaa aataatttcc agttctgcaa gaatctgatt
     4321 tttttctaat ggcactggca cggtaaatga aattggaaaa gttatgctta acatatcaga
     4381 atctgtatga gaaacaccga tcttatgctg tgaatttttt ttatggagta taaattcttg
     4441 ccatcttgta actctttttt ttacttcatc aattttagtt tgtagtaaat ctaaatcaat
     4501 ttcacgatct ccaccaaaca ctccaaactt taccattgga accatgagat agcctccaga
     4561 aattttgtca tgccttttgt gcatttcttc tattatttca tataccttgt gttcaggtct
     4621 aatttctcca tattcaggat caaaatatcc tgcaatgttt ttattctcat caaaaatctt
     4681 aaaaaattca tcttcgcgct ctaatttcca tttcatgtat gtcagtaaaa agtatcagtt
     4741 aataatttta ggcttctttt ggaactgcgt tcaggcattt tccacaaaag tattcttttc
     4801 cagtactgaa tgtggtagtg ttattacact gcggacatgg tctgtttatt cgattcattt
     4861 ctattggcat gatacgaaat ttgcgatcta gctaaaaaga aactgggtca aattatgtta
     4921 gtaatttctc taaaatcaac tattataaat gataattctg aatcaaccat gtggccataa
     4981 ctccatcaac taaagacttt attgaaaacc tcataaatta ctacgttagc gaggcacctt
     5041 cttacagaga gattgcagaa atttataccg ctgaggtcaa aaacatacca gatacaactt
     5101 ttggaataat actaggatgc gtatattctg gttttttgca aacatataca aaccaaaaac
     5161 agactcccgc tcttgaggat attcaggaat ttaatcaaat tatcaaacag agagcatcag
     5221 agataaaaaa agcaatacta gacaaatcat aaatctacgt tagagataaa ttctaacttt
     5281 tttaatgaaa atcatgtcag agacagtaca ttacaagata caacaactag tagataatgg
     5341 tcaaatcgta caaatttcgc ttgttgaaga tgtacagcta gagccagttt cacagaagca
     5401 gataatgtta gatgccataa ataaaaaact agatccagaa ctaaaagaac aggtcatgcc
     5461 actaattgaa gcaataatgc aagcacagcc aaccattgca gtaaaaagct atgcgcaaac
     5521 ttcaattaca atttccatgc caaggcgcag atatgaaatg attggtagtc caagagttgg
     5581 agaaaaatta gaaattaacg taaaaaaaat ttcttaaatt aatttttcga tctcagataa
     5641 attatcaagt ggtaatgaac aggtaaaatc tttgcagaca aatacaatag ttttgttttt
     5701 atcaaattgt tttcctgcga aaaactgata atttgcaagt tttgttagtt gttctttgtt
     5761 tcgtattgtt acaaatattg attctggaag gaattttttt actaaagcat agtaaatttc
     5821 atgatctttt gtgtttaaca aggttatttc tattggcttt tttaggtaca aatacatagc
     5881 atttagaagc tgtccaaagc caaatggatt ttctgcggcc atgattgcaa gcgattccat
     5941 aattttcgta gaaacatcaa gaaacttctt gtcttgtgtc agatgataga gttttaagaa
     6001 cgcatatgca gcaacagaat taccagatgg cagagaaaga tcgtagagat tttttgttct
     6061 aatgattagt ttttcatgat catcagcagt aaaaaagaaa ttgttttcag attcatccca
     6121 aaagtggtct attaaataat ctgcatgttt tacagcaaga tctagatatt ttgcatcagg
     6181 atttatctca aagacatcca ataacgcatt tacaaaatat acatagtcat caagatatgc
     6241 cattagttta gcttgaccgt ttttgtacgt tcggagaagt ttaccatttt ttgttagttt
     6301 ttcttctata aatgtaatac agttttgtgt agcatcaaga aactcattca agccgcttat
     6361 cctatatcct ttggcaaatg cggaaatcat tagtccattc caactggtca gaatcttgtc
     6421 atctcttccc ggtgcaattc ttttagagcg a
//
LOCUS       DATIDL010000076         6397 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrosopumilaceae archaeon isolate SMAG_U16342
            k141_2112205, whole genome shotgun sequence.
ACCESSION   DATIDL010000076 DATIDL010000000
VERSION     DATIDL010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935836
            Sequence Read Archive: SRR25158326
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosopumilaceae archaeon (soil metagenome)
  ORGANISM  Nitrosopumilaceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrosopumilales;
            Nitrosopumilaceae.
REFERENCE   1  (bases 1 to 6397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/04/2024 05:09:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,520
            CDSs (total)                 :: 1,474
            Genes (coding)               :: 1,474
            CDSs (with protein)          :: 1,474
            Genes (RNA)                  :: 46
            rRNAs                        :: 1 (5S)
            complete rRNAs               :: 1 (5S)
            tRNAs                        :: 43
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6397
                     /organism="Nitrosopumilaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2112205"
                     /isolate="SMAG_U16342"
                     /isolation_source="soil"
                     /db_xref="taxon:2202732"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..314
                     /locus_tag="VLB45_03290"
     CDS             <1..314
                     /locus_tag="VLB45_03290"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HSD04759.1"
                     /translation="KIKSDPNYTIYVAVLDSKVIGATTLLIEQKFIHKGGLVGHIEDV
                     VVRKEYQGKGIGQKLVKALSDYAEKKGCYKTILDCTDDLVPFYEKIGFKRNSNSMRID
                     Y"
     gene            complement(317..736)
                     /locus_tag="VLB45_03295"
     CDS             complement(317..736)
                     /locus_tag="VLB45_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010193493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04760.1"
                     /translation="MAVEKWVGLASLGLYIMFVAEILTILIYLVNPTHEIQPGGKILQ
                     YISISVAPALVLSGTAFLMSRRFGSRIIGSMIVSGGAILLVGMYYASTVLSSIANQYL
                     VPEVTIVPPLFMVVSIPVMITGALLFRTKPKPKKQYY"
     gene            complement(796..1815)
                     /locus_tag="VLB45_03300"
     CDS             complement(796..1815)
                     /locus_tag="VLB45_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HSD04761.1"
                     /translation="MGDLRKDYTIERFVIVSPDNHRVVADPKKCPFCPGNEAMTNPSS
                     LSLVAKDGMLQRLQDSEDYYVEGWSVRVFESKNPVVSTTSENSYSDRPLYSEPAYGYH
                     YIVVASEKHKDSLSTIPVEQWSNVLVVIQDRLRWLYTQRGVTYVSIYVNHGKEAGGFT
                     THPHLNVVTFSTIPPVIEQEAEASHKILNEKGTCPMCQTVSTETGGPRQILQTEGFLA
                     FSPWAPSHPYEFWIYPKKHITSFSKITQKEINDLSLILRATLGGLANTTKNTSYNMVF
                     HLSPEKKNNRQIHWHIEVYPQSDYWSGLERGFGVFLNKTTPEKVAEELGSSCRKELSA
                     LVGIV"
     gene            1950..3206
                     /locus_tag="VLB45_03305"
     CDS             1950..3206
                     /locus_tag="VLB45_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007550002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HSD04762.1"
                     /translation="MIYVLLDGVGDLPHPDLKGSTPLSFAKTPNLDKIARNGSIGQVI
                     SVGKGIAPQSDIAVFNMLGYKFLHDYVGRGVIEAIGIGIDFRDGDLALRGNFSTINDE
                     GIITDRRAGRNIEKEDALGVSLEIERLVRFSNPKASVVVAPTIGHRVIVRIRCDGESL
                     SSEISNTDPAYGRIDGMGVAKAVGDYLKIERCLPLNESPSAKLTATFVNEFTEQSIAI
                     MKKSEINKMRKEKGKKVLNSILLRDAGNRFPKLTPINEMHSMKFSCIVDMPVEIGISN
                     VLKMKTFSAGGLTDYEEKARVAAKAMETQNAIYVHIKGPDEFGHDGDAIGKMKNIEEI
                     DERFFGTLLNNIDTSKVAIVISADHSTPCINKAHSADPVPVLVSGDAIKKDGTARFTE
                     EEAKKGKIGLLAGADVIKTAIQLIKS"
     gene            complement(3196..3747)
                     /locus_tag="VLB45_03310"
     CDS             complement(3196..3747)
                     /locus_tag="VLB45_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007550001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF309 domain-containing protein"
                     /protein_id="HSD04763.1"
                     /translation="MIHLKNSRHVLPQDSSKLLTQARHLAAGMNITIRDMRVSSRYLE
                     FDASIEKERLDELVRKLESIGPLDHAKHVVEEPIEKNDAIEKGRFYFNNERFWESHEI
                     LEGAWKKSEGKEKELIQGIILVAAALVHYQKDENEICLSIFRRALEKIGNATGKYHKI
                     DIDDLRNKVSNMIRTGVLETFTI"
     gene            3843..4319
                     /locus_tag="VLB45_03315"
     CDS             3843..4319
                     /locus_tag="VLB45_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04764.1"
                     /translation="MSMIETTRDVKNNLLSRREITCTFKGLGGKLKKLEAIDMIAKQF
                     KLEGKVIVPITLKNETGRPMVSGTFYVYDDEKLAKQHIKPAIFTRLEKAKGGGKTEEE
                     TKEAAPAEAKEAAPAKTKDAPAKAKEEAPAKTKEAPVEKKEEKSEKKPEEKKEKSK"
     gene            4319..4492
                     /locus_tag="VLB45_03320"
     CDS             4319..4492
                     /locus_tag="VLB45_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S27ae"
                     /protein_id="HSD04765.1"
                     /translation="MAGKKGSSPSVYKFYKVKGDKAERMRKECSRCGKGVFMSEHKNR
                     RTCGKCGLTEFIQ"
     gene            complement(4493..5200)
                     /locus_tag="VLB45_03325"
     CDS             complement(4493..5200)
                     /locus_tag="VLB45_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04766.1"
                     /translation="MDPLLKVHEAHKNGLLPDKIHSLIVDRFPVAVAGIDRIEKASGM
                     NFPLAYVEPSIVLTHANTSSFDYGILFARTIPVVTGDTLQVVIQITAPLVAYGLKGTI
                     HAILAHEFLHYLELVRKVSKMDFVSDEISGNLFEGIYSDTTRLFEPRAVFDDKTLLLH
                     ITKKFPEGFRDYKLEDKVVKNWIEKKLPTTNITLDTNIAKIPANVIANTKVDPKILSK
                     LDEFEQKASKLRKKKLY"
     gene            complement(5258..6394)
                     /locus_tag="VLB45_03330"
     CDS             complement(5258..6394)
                     /locus_tag="VLB45_03330"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0031119 - tRNA pseudouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(54/55) synthase Pus10"
                     /protein_id="HSD04767.1"
                     /translation="MISDANAILKEHKLCDNCLGRLFAQKLGVSSHKILGSKIRNILK
                     KNVPKSCYICKNTISKLDTQLNKMLEMSHDYEFSSFLVGAILQPSILDRDDLIRSKFK
                     LKGTNSIKGDINREIGKRFGRKTRTSVDYQNPDIVFTLDFKNEECEIKPKPVFLSGRY
                     TKSIRSIPQKQRPCKNCKGKGCYVCEFHGISEFDSVEGKIAKLLYKKFGAQQIKITWI
                     GSEDETSLVLGKGRPFFVKLVNPHKRRLKLGKKITLGDVTIHGLKVIGKIPSNIMRFK
                     SKVEMEVQTQNEINPEQLKNLRQLKKQPISIYENSGKRSLRRIYDIRYKKKSNNSFSL
                     FIKADGGVPLKRLADGVEVTPSLSSILENPCKCKVFDFHEIVLN"
BASE COUNT         1995 a         1185 c         1289 g         1928 t
ORIGIN      
        1 agaagataaa atctgatcca aactatacaa tctacgtggc agtgcttgat tcaaaggtga
       61 ttggagctac aactctgctc atagaacaaa aattcattca caaaggaggg ctggttggac
      121 acattgagga tgttgttgta agaaaggaat accaaggcaa aggcattgga caaaaattag
      181 taaaggcgct ctcagactat gcagaaaaga aaggatgcta caaaaccata cttgattgca
      241 ccgatgatct tgtgccattt tacgaaaaaa ttggctttaa acgcaattcc aattctatga
      301 gaatcgatta ttgattctaa tagtattgtt tttttggttt gggtttagtt ctaaacaaca
      361 gagcaccggt tatcatgaca ggtattgata ctaccatgaa aagtggtgga acaattgtga
      421 cttcgggaac caaatattgg tttgcaatac tagacagcac cgtgcttgca taatacattc
      481 caactaaaag tatagctcct ccagaaacta tcattgaacc gattattcta gatccaaacc
      541 gacgtgacat caaaaatgct gttccagaaa gaaccagagc tggtgcaacg ctaattgaga
      601 tgtattgcag tatttttcca ccaggttgga tttcatgagt aggatttaca agataaataa
      661 gaattgttaa aatttcagca acaaacatga tgtataatcc caagcttgca agaccaaccc
      721 atttctcaac tgccatgcgt tacaattttt tggttcaata aataaaccaa gtgatcgcga
      781 ttttaatttt taatgctaca ctatgcctac aagagcagac aattcttttc tacaagacga
      841 accaagttct tctgcaactt tttctggagt agttttattt agaaagacac caaatcctcg
      901 ttctagtcca gaccaataat cagactgcgg ataaacttcg atgtgccaat gaatctgtct
      961 gttgtttttc ttttcagggg acagatgaaa caccatgttg taagatgtat tttttgttgt
     1021 atttgccagt ccgccaagtg tcgcgcgcaa aattaatgaa agatcattaa tctctttttg
     1081 cgtgatcttt gaaaaactgg ttatgtgttt tttgggatat atccagaatt catacgggtg
     1141 tgaaggcgcc catggcgaga atgcaagaaa accttctgtc tgaagtatct gtcttggccc
     1201 accagtttcg gtactaactg tctgacacat tgggcatgtt cctttttcat ttaggatttt
     1261 gtgagaggct tcggcttctt gctctatgac aggcggaatt gttgaaaatg tgacaacgtt
     1321 aaggtgagga tgcgttgtaa atccacctgc ctctttaccg tggtttacgt agattgagac
     1381 gtatgtgact cccctttggg tgtaaagcca gcgtaatctg tcctgaatta caacaagtac
     1441 gttggaccac tgttctacag gaatggttga aagtgaatct ttgtgttttt cagatgctac
     1501 aacaatgtaa tgataaccgt atgcaggttc gctgtaaagc ggtctgtcac tgtaagaatt
     1561 ttcagaggtg gtagacacaa ctgggttttt gctttcaaaa actctgacag accaaccctc
     1621 aacataataa tcctcgctgt cttgtagtct ttgcaacatt ccatcttttg ccacaagaga
     1681 aagtgatgaa ggatttgtca tggcctcatt tccaggacag aacggacatt tttttggatc
     1741 tgcaacaact cggtggttgt cgggagaaac aataacaaat ctctcaatag tgtaatcctt
     1801 gcgcagatcc cccatatcac tagcaatttg atctagtaat ttaaagcctt ttcaaagatc
     1861 gagtcttaaa aaatattgta aagtttatgt gtgacacaag aaactcgaaa gtgagctgag
     1921 cagaaattag tgacaattct gatgttcata tgatctatgt tcttttggac ggagtcggag
     1981 atcttccaca tccggatctt aaaggcagca ctccacttag ttttgcaaaa actccaaacc
     2041 ttgacaagat tgcaagaaac ggctcaatag gccaagtgat ctcagttgga aaaggcattg
     2101 caccgcaatc tgacattgca gttttcaata tgttaggata caagttctta catgattatg
     2161 tcggcagagg agtaatcgaa gcgataggaa taggcataga tttcagagac ggagatcttg
     2221 ccctgcgagg aaatttttct accataaatg acgagggcat aataactgac aggcgggcag
     2281 gcagaaacat cgaaaaagaa gatgccctgg gagtgtcact tgagatagaa cgtcttgtca
     2341 ggttttcaaa tccaaaagca tcagtggttg ttgcaccaac tattggacac agagtaatcg
     2401 taagaataag atgtgatgga gaatcgcttt cttcagagat tagcaacacg gatccagcat
     2461 atggtagaat agacggaatg ggtgtcgcaa aagcagttgg agactatctt aagattgaaa
     2521 gatgccttcc tctaaatgag tcacccagtg caaaacttac tgccactttt gtcaatgaat
     2581 ttaccgagca atcaattgcc atcatgaaaa aaagtgaaat taataaaatg cgaaaagaaa
     2641 aaggcaagaa agttttaaac agcatacttt tgcgtgacgc aggaaataga tttccaaaac
     2701 ttaccccaat aaacgaaatg cattcaatga agttttcttg catcgtagac atgccagtgg
     2761 agattggaat ttcaaatgtc ctaaagatga agacgtttag tgctggaggc ttgacagact
     2821 acgaagaaaa ggcaagagta gctgcaaaag caatggaaac acaaaatgca atatatgtac
     2881 acatcaaggg ccctgatgaa tttggacacg acggggatgc aattggcaag atgaaaaaca
     2941 ttgaagagat agacgagcga ttttttggca cgttgcttaa caacatagac acgtcaaaag
     3001 tagcaattgt gatttcggct gatcactcta ctccatgtat aaacaaggca cacagtgcag
     3061 atcctgtacc ggtgctggtt tctggcgatg ccattaaaaa agatggaacc gccaggttta
     3121 ctgaagaaga agcaaaaaaa ggcaaaatag gattgcttgc aggtgcggat gtgattaaaa
     3181 cggcaataca gctgattaaa tcgtaaaagt ttccaaaact ccagttctga tcatgttaga
     3241 aactttgttg cgcagatcat cgatgtctat tttatggtat ttcccagttg cattgccaat
     3301 tttttccaga gcacgcctaa aaattgaaag gcagatttca ttttcatcct tttgataatg
     3361 aaccagtgca gctgcgacaa gaattattcc ttgaatcagt tctttttctt ttccttcaga
     3421 ttttttccat gccccttcca agatttcgtg tgactcccaa aatctttcgt tattgaaata
     3481 gaatcttcct ttttctattg cgtcgttttt ttctattggt tcttcaacaa cgtgttttgc
     3541 atgatcaagt ggtcctattg actctaattt tcgtacaagt tcatccagac gttctttttc
     3601 tatagaagca tcaaattcca aatatctgct tgagactcgc atatctcgta ttgtaatatt
     3661 catgccagct gcaagatgac gagcttgtgt aagaagtttt gacgaatctt gtggaagaac
     3721 atgccttgaa tttttcaaat gaatcataaa acgatccata acaaaacaaa gttgtaattt
     3781 cttaaatctg ttagctattt tctgttaaga tttatatgaa ctatcaggcg gacttgggat
     3841 acatgtctat gatagagacc acacgggatg taaaaaacaa cctgctctca agaagagaga
     3901 ttacgtgtac gtttaagggt cttggtggca agctgaaaaa acttgaagcc atagacatga
     3961 tagcaaaaca attcaagttg gaaggaaagg taatagttcc aatcacatta aaaaatgaaa
     4021 ccggcagacc gatggtttct ggcacattct acgtttatga cgacgagaaa cttgcaaaac
     4081 aacacatcaa accggcaatt ttcaccagat tagaaaaagc aaagggcgga ggaaagacag
     4141 aagaagagac aaaggaagct gcacccgctg aagcaaaaga agctgctcct gccaagacaa
     4201 aagatgcccc tgccaaagca aaagaagaag caccagctaa aacaaaagaa gccccagtag
     4261 aaaagaaaga agaaaagtcg gaaaagaaac ccgaagaaaa aaaggaaaag agtaagtaat
     4321 ggcaggaaag aaaggttcca gtcccagtgt ttacaagttt tacaaagtaa aaggagacaa
     4381 ggctgaaaga atgagaaaag aatgctccag atgtggcaaa ggagtcttta tgtcagagca
     4441 caaaaataga cgcacatgcg gtaaatgcgg attaactgaa tttatccagt aatcagtaca
     4501 attttttctt tctaagttta gaagcctttt gctcaaactc gtcaagtttt gataaaattt
     4561 ttggatctac tttcgtgtta gctatcacgt ttgcgggaat cttggcgatg tttgtgtcca
     4621 gtgtgatgtt tgtggtaggg agtttttttt ctatccagtt ctttactacc ttatcttcaa
     4681 gtttatagtc tctaaatccc tctggaaatt ttttcgtaat gtgcagcaac aaagttttgt
     4741 catcaaatac tgctcgtggc tcaaagagtc ttgtggtatc agagtatatt ccctcaaaga
     4801 gatttccgga aatctcatct gagacaaaat ccatttttga cactttgcga acaagttcca
     4861 aataatgcag aaattcgtgc gcaagtattg catgtatggt gcctttcagc ccatatgcta
     4921 caaggggtgc agtaatttgg ataacaacct gaagagtatc accagttaca actgggattg
     4981 ttctagcaaa caggattcca taatcaaagg aactcgtatt agcatgtgta agtacgatag
     5041 atggttccac atatgcaaga ggaaaattca ttccagaggc tttttctatc ctgtcaattc
     5101 ctgcaacagc cactgggaat ctatcaacaa taagagaatg aattttgtca ggcaagagtc
     5161 catttttgtg cgcctcatga acctttagta atggatccaa tggatgaaag ctcgaatcag
     5221 actataaaaa acgttagtac tgtgaataaa tcaaaaccta attcaatacg atctcatgaa
     5281 aatcgaaaac tttgcacttg catgggtttt ctagtatgct gctcagacta ggggtaacct
     5341 caactccatc tgcaagtctt tttagtggaa caccaccgtc tgcttttata aaaagtgaaa
     5401 aagaattgtt agactttttt ttgtaacgta tatcgtatat gcgtcttagg cttcgctttc
     5461 cagagttttc ataaattgat attggttgct ttttcagttg gcgtagattt tttagctgct
     5521 caggattgat ttcgttttga gtttgtactt ccatttccac tttggacttg aatcgcataa
     5581 tgtttgatgg gatctttcca attaccttaa gaccgtgaat agtcacgtcg ccaagagtga
     5641 tttttttacc tagttttagg cgtcttttgt gtgggtttac aagctttaca aaaaatggcc
     5701 tgccctttcc caaaaccaga cttgtttcat cctcactacc aatccatgta atcttgattt
     5761 gttgtgctcc aaactttttg taaagaagct tcgctatttt tccttctaca ctatcaaatt
     5821 cagatatgcc atgaaattcg catacataac atccctttcc cttacagttt ttgcatggcc
     5881 tttgtttttg cgggattgat cgtatgcttt tggtatacct accagaaaga aagactggct
     5941 ttggtttaat ttcacattct tcatttttaa aatccagcgt aaatacaatg tctggatttt
     6001 ggtaatctac actggttctg gtttttcttc caaatctttt tccgatttct ctgttgatgt
     6061 cgccttttat gctgttggtt ccttttagtt tgaattttga gcggatcaga tcatccctgt
     6121 ccagtattga tggctgcagt attgcgccca caagaaaaga cgagaattca tagtcatgag
     6181 acatttcaag cattttgttt agctgcgtgt caagttttga tatggtattt ttgcagatat
     6241 agcagctttt tggtacgttt tttttcaaaa tatttctaat tttgcttcca agtattttgt
     6301 gcgatgatac gccaagtttt tgggcaaaca atcttccaag acaattatcg caaagtttgt
     6361 gttcttttaa aattgcattt gcatcagaaa ttattaa
//
LOCUS       DATIDL010000077         6939 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrosopumilaceae archaeon isolate SMAG_U16342
            k141_2719795, whole genome shotgun sequence.
ACCESSION   DATIDL010000077 DATIDL010000000
VERSION     DATIDL010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935836
            Sequence Read Archive: SRR25158326
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosopumilaceae archaeon (soil metagenome)
  ORGANISM  Nitrosopumilaceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrosopumilales;
            Nitrosopumilaceae.
REFERENCE   1  (bases 1 to 6939)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6939)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/04/2024 05:09:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,520
            CDSs (total)                 :: 1,474
            Genes (coding)               :: 1,474
            CDSs (with protein)          :: 1,474
            Genes (RNA)                  :: 46
            rRNAs                        :: 1 (5S)
            complete rRNAs               :: 1 (5S)
            tRNAs                        :: 43
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6939
                     /organism="Nitrosopumilaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2719795"
                     /isolate="SMAG_U16342"
                     /isolation_source="soil"
                     /db_xref="taxon:2202732"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..238
                     /locus_tag="VLB45_03335"
     CDS             <1..238
                     /locus_tag="VLB45_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008301329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04768.1"
                     /translation="KDFRDQAQPMSPFGSNVMGFSNMDDMVKGLNKMMSGGAFGQAGN
                     ITSIKLDMHEYKELGLSVGDKIFLDISKPETLGV"
     gene            complement(250..912)
                     /locus_tag="VLB45_03340"
     CDS             complement(250..912)
                     /locus_tag="VLB45_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010191859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HSD04769.1"
                     /translation="MRTANVTIPDAVREIITRNRSIYDCVKMDLINYTALAVKIQKEV
                     EDYVGSPVNLNTVVVAIKRYADSFERKEEIQNEPVLKNARLSLTDGIADIKFSTDNFG
                     IDDTVDLLNKFTQIDPNYEFFRLTDSFRILTEDVLDVRKLLESFPNQKNIFQTGLAKI
                     SIRISGVQNRSDVVSFVSEILHDNGIEIVNAYFGQDDIIMVLQEKDAARAYQILRSEI
                     SK"
     gene            1024..1524
                     /gene="hsp20"
                     /locus_tag="VLB45_03345"
     CDS             1024..1524
                     /gene="hsp20"
                     /locus_tag="VLB45_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016939439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaeal heat shock protein Hsp20"
                     /protein_id="HSD04770.1"
                     /translation="MFPTDEFDNLWRRMSRQFMDIDDIFDDLKNTDNLQTFGPYYYGY
                     TMTVGSDGKPIVKEFGNVKPTMLPTSDIREPFVDVLVDDKEKVLKLIAEMPGVEKKDI
                     KIIVEGRTVNLDAVHGEKKYHAKVPIRHKVDEDSVKALYANGILEVRFKLKEEEKPKG
                     KTVEVE"
     gene            1555..3690
                     /locus_tag="VLB45_03350"
     CDS             1555..3690
                     /locus_tag="VLB45_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008297191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDC48 family AAA ATPase"
                     /protein_id="HSD04771.1"
                     /translation="MSEIILKIAEAPQRLVGKGIAVVDPKVVEDNKWETGQILEIVGN
                     RKSHVKLWPGATEDYGNGIIKIDGLTRHNIGSGIGEKISIKNVDAKWAEQVVVSPIEK
                     LSEEGLQEYMQANYDGHVLTSGDTLIVNTQLGSKTQLIVTNTTPAGKPVIVTDQTEFK
                     LGSMTKAIDQSVPRITYDDLGGLKKEVQKVREMIELPMRHPELFEKLGVEAPKGVLLY
                     GPPGTGKTLLAKAVAGETNSHFISLSGPEIMGKFYGESEERLRDIFKQAEENAPSIIF
                     IDEIDSIAPKREEVTGEVEKRIVSQLLTLMDGMKSRGKVVVIAATNRPDSLDPALRRP
                     GRFDREIEIGIPDESGRKEVLNIHTRGMPLDDKVNLDQIAKVTHGFVGADLEILAKEA
                     AMRSLRRILPELDLEEEKIPLEFLQKIVITDDDFKDALKEVRPSALREVLVQVPNVTW
                     DEVGGLESLKEELRESIEWPLKHKEAFEHTNVSPPKGILLHGPPGTGKTLIAKAVAHT
                     TESNFISIKGPELLSKWVGESEKGVREIFRKARQAAPCIIFLDEVDAIAPSRGSGSSD
                     SNVTERVVSQILTELDGLEELHNVVVIGATNRLDIVDAALLRPGRFDRIIEVPLPDAK
                     GRENIFRIHTKKKPLADNVDLAKLVEQTEGYSGAEIEGICNRAAMSAIKRFVNNKEKS
                     VKSIKITQEDLVNTIQKVKPEKRKMSAVA"
     gene            3722..4783
                     /gene="amrS"
                     /locus_tag="VLB45_03355"
     CDS             3722..4783
                     /gene="amrS"
                     /locus_tag="VLB45_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HSD04772.1"
                     /translation="MTTREAILYEKLPNEKVRCTACARYCEIGKGQIGLCGIRGNEAG
                     KLELFVYGKVIAGNVDPIEKKPVIHYRPGTSIFSIATTGCNWLCKYCQNYDISQRRKI
                     EGTDMTPEEVANQALKLGADGIAYTYNEPSVFIEFARDCGVAARKKGLFNIFVSNAYD
                     TPESVKMMNEFLDCITVDFKGNAEPQFSRKYIGIPDPKPIFDTLIEIRDKTKIHVEIT
                     DLIVPQVGDNLEYATKLCKFIYDEFGPEMPIHFLRFHPDYKMMEFEPTPVKTLEKHYE
                     IAKKEGLSYVYLGNVPGHPLEHTYCSQCNNIVVKRYGFDIQGWNMDENNNCKFCGNKI
                     PITGKLPKNYKQSRFQFVY"
     gene            4852..5112
                     /locus_tag="VLB45_03360"
     CDS             4852..5112
                     /locus_tag="VLB45_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007551635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HSD04773.1"
                     /translation="MPTTYILLNSDLGSDVEIIKKIKEILETEGKAVKYEVQGVYGVY
                     DIVVKITSDNMDHLRNIITNKIRKIDKVYSTLTMMVIEEQEQ"
     gene            complement(5114..6262)
                     /locus_tag="VLB45_03365"
     CDS             complement(5114..6262)
                     /locus_tag="VLB45_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HSD04774.1"
                     /translation="MNSDYDVAQDFPFKSRIYLNNASTSLMPISSIKAMTDFLVTYNE
                     LGPDSSTADNFVREKLFLARKAISRLINCNPEEVILTQSTTDGVNIVANGLKLEKKSN
                     IITRGASHEHPANHYPWIRLSEKCEIRNLQVNETGYFEFEHLKETIDNNTGLVALSHA
                     LFNTGAILPVEKVGELLATNNIPFFVDSAQTVGCVENVDVNKIKCNFMSFNGSKWLCG
                     PMGTGIFFCKKESSELLEPLQVGGESAFLHENKITHKEMPSKFQAGFRNWAGVAGLEA
                     SAVYLLRLGLGNIRQKNIKLADILREQLARIPGITLYGPEESEKRTSIVSFSIQDQDP
                     KTVVERLEKQNMILAVREIFSKKVIRASMHFFNTESEIQKVVEAIKKL"
     gene            6290..6913
                     /locus_tag="VLB45_03370"
     CDS             6290..6913
                     /locus_tag="VLB45_03370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04775.1"
                     /translation="MLLKNHDNDVTKNVVLDLDDILSQSKINSVLYSDPFLKAGFLAK
                     LVQDTKIDVLYLDLDLLYSGYLASEILGTPQNLVLFQPTLELWNQMLTDVLVKLSNKK
                     SLVVIDSLNGLYNILNERKEVGKLVTSYIMLITSIAKITNSYIVVASMVRFKKEEGWV
                     LSPTGKRLIETSMAKKILLGQNDSEITLDFLKEKKKITIPLNAIPLV"
BASE COUNT         2427 a         1210 c         1295 g         2007 t
ORIGIN      
        1 taaggatttc agggatcagg cacaaccaat gtctcctttt ggttcaaacg taatgggatt
       61 ttcaaatatg gatgatatgg taaaaggtct gaataaaatg atgtctggtg gggcatttgg
      121 acaggctggt aacataacct ccattaaact ggacatgcat gaatacaaag agcttggcct
      181 ttctgtgggc gacaaaatct ttcttgacat ttccaaacca gaaacacttg gtgtataaac
      241 accctagatt tattttgaaa tttcagaacg caaaatctga tatgccctgg cggcatcctt
      301 ttcctgcaaa accataatga tatcgtcctg tccaaaatac gcattaacaa tctctatgcc
      361 attatcatgc aagatttccg atacaaaaga gaccacatct gatctatttt gaactcctga
      421 aattcgtatg cttatctttg caagtccagt ctggaaaata tttttctggt ttgggaatga
      481 ttccaacagc tttctcacat ctaatacatc ctcagttaat attctaaaag agtctgtcag
      541 cctgaaaaac tcataattgg gatcgatttg agtaaatttg tttagcaggt ctacagtatc
      601 atctatccca aaattatccg ttgaaaattt aatatcagca attccatctg tcaaagagag
      661 tcttgcattt ttcagtactg gttcattttg aatttcttct tttctctcaa aagaatctgc
      721 gtatcttttg attgcaacta ccaccgtgtt tagatttact gggctgccaa cataatcttc
      781 aacttctttt tggatcttta ctgccaatgc agtatagttg atcagatcca ttttcacaca
      841 atcataaatc gaacgatttc tagtgattat ttctcgtact gcatcaggaa tggtgacatt
      901 tgctgttcgc atctcaagct tattagtagt gtcatatata atagccttat gtaagtttta
      961 ggtatattat acaataaaat atatataatg tcataactat tacaaactat aggtgattat
     1021 gacatgttcc caacagacga atttgataac ctttggcgaa ggatgtccag acagtttatg
     1081 gacatagatg acatctttga cgatctaaaa aacacagaca accttcagac atttggtcca
     1141 tactactacg ggtataccat gacagtagga tcagatggga aaccaatagt caaagaattt
     1201 ggaaacgtca aacctaccat gttaccaaca tctgatattc gagaaccatt tgtagatgtt
     1261 cttgtagacg acaaggaaaa ggtattgaag ttaattgctg aaatgcctgg agtcgagaaa
     1321 aaggacatca aaatcatagt tgaaggaaga acggtaaatc ttgatgcagt acatggtgag
     1381 aaaaaatacc acgcaaaagt accgataaga cacaaagtag atgaagattc tgtcaaggca
     1441 ctttatgcaa acggaattct tgaagtacgt ttcaaattaa aagaagagga aaaacccaaa
     1501 ggtaagaccg tggaggttga ataaactcca tttctttttt atggtgatta gattatgagc
     1561 gaaataattt taaaaattgc agaggctccg caacgactag taggaaaagg aattgctgta
     1621 gtggatccta aagttgtaga agacaacaag tgggaaactg gccagatttt agaaattgtt
     1681 ggtaatagaa aaagccacgt caagttatgg cctggtgcta ctgaagatta tggtaatgga
     1741 ataatcaaaa ttgacggtct aacaagacat aacattggtt ctggcattgg cgaaaagatt
     1801 tccataaaaa atgtagatgc aaaatgggca gaacaagtag tagtgtctcc tattgaaaaa
     1861 ctaagtgaag aaggattaca ggaatatatg caagcaaact atgacgggca tgttttaaca
     1921 tccggtgata ctctcatagt caatacacag ctaggcagca aaacacagtt aattgtaacc
     1981 aataccactc ctgcaggaaa accagtcatt gtaactgacc aaaccgagtt taaactaggc
     2041 agtatgacaa aggcaataga ccaatctgtc ccaagaatta cttatgacga tctaggcggt
     2101 ctaaaaaaag aagttcaaaa agtgcgtgag atgattgagc ttcccatgag acatcctgaa
     2161 ttatttgaaa aacttggtgt tgaagctcca aaaggcgtat tgttgtatgg accaccaggt
     2221 actggaaaga cacttttagc aaaagcagtt gcaggagaaa ccaattctca ttttatctct
     2281 cttagcggtc cagaaatcat gggcaaattt tatggtgaaa gcgaagaaag attgcgtgat
     2341 atattcaaac aagccgaaga aaatgctcca agcataatat tcattgacga gattgattct
     2401 attgctccaa aaagagaaga agtaactggc gaagtagaaa aaagaatagt gtcgcaatta
     2461 cttaccttga tggatggaat gaaatcaagg ggcaaagttg ttgtaattgc agcaacaaac
     2521 agacctgatt ctcttgatcc agcactaaga aggccaggtc gattcgacag ggaaattgag
     2581 attggcatac cagacgaatc tggaagaaaa gaagttctta acattcatac tcgtggaatg
     2641 ccacttgatg acaaagtcaa tctagatcag attgccaaag tgacacatgg atttgtaggg
     2701 gctgatttag aaattcttgc aaaggaagct gcgatgagat cattaagaag aattttgcct
     2761 gaattagatc tagaagagga aaaaatacca ttggagtttt tacaaaaaat tgtaatcacc
     2821 gatgacgatt tcaaggatgc attaaaagaa gtcagaccat cagcattacg tgaagtacta
     2881 gttcaggttc caaacgtaac ttgggatgaa gtaggcggat tagagtcact caaagaggaa
     2941 ctacgtgaat ccattgaatg gccgctaaaa cataaagaag catttgagca cacaaacgtc
     3001 tctccgccaa aaggaatttt gttgcatggg cccccaggta ctggtaaaac actgattgca
     3061 aaagcagttg ctcacactac agaatctaat ttcattagca tcaaaggacc agaactctta
     3121 tcaaaatggg taggagaatc tgaaaaagga gtaagggaaa tctttagaaa agcaagacaa
     3181 gccgcaccat gtataatatt tctagacgaa gtggatgcta ttgctccaag cagaggctct
     3241 ggaagttcag attcaaatgt aactgaaaga gtagtatcac aaattctaac agaattggat
     3301 ggtctagaag agctacacaa tgttgtagtc attggtgcaa ccaacagatt agacattgta
     3361 gatgccgcac tactacgacc aggtagattt gatagaataa ttgaagtgcc attacctgat
     3421 gcaaaaggac gagaaaacat cttcagaata catacaaaga aaaaacctct agctgacaat
     3481 gtagatcttg caaaacttgt agaacaaaca gaaggataca gtggtgcaga aattgaagga
     3541 atctgcaaca gagctgcaat gagtgcaatc aagagatttg taaataacaa agaaaaatct
     3601 gtcaaatcaa tcaagataac tcaagaagat ctggtaaata caattcaaaa agttaaacca
     3661 gaaaagagaa agatgtctgc agtagcctga tagcttatat gacaaattcc tgttatagta
     3721 tatgacgact agagaagcaa tactttacga aaagctacct aatgagaagg ttagatgtac
     3781 agcttgtgca agatattgtg aaattggtaa aggacagata ggcctttgtg gtatacgcgg
     3841 aaatgaggct ggaaagctag aactatttgt ttatggtaaa gttattgctg gaaatgttga
     3901 ccctatagaa aaaaaacctg tgatacatta tcgtcctggc acaagcatat tttctattgc
     3961 cacaaccggg tgtaattggt tatgcaagta ctgccaaaat tatgatatta gtcaaagacg
     4021 aaaaatagag ggtactgata tgactccaga agaggtagct aatcaagccc tcaaactagg
     4081 agccgacgga attgcgtaca catacaacga gccatctgtt tttatcgaat ttgcacgtga
     4141 ttgtggagtt gctgcaagaa aaaaagggct attcaacatc tttgtgtcaa atgcatatga
     4201 tactcccgaa tcagtaaaga tgatgaatga atttcttgac tgcataaccg tagattttaa
     4261 gggaaacgca gaaccacaat ttagtagaaa atacattgga atacctgatc caaaacccat
     4321 ctttgataca ctgatagaaa tcagagacaa aacgaaaatt cacgtagaaa tcacagatct
     4381 tattgtacct caagttggag ataatctaga atatgcaacc aaattatgta aattcatata
     4441 tgatgaattt ggtccggaaa tgccaattca tttcttacga tttcatcctg attacaaaat
     4501 gatggaattt gaaccaactc cagttaagac acttgaaaaa cactatgaaa ttgcaaaaaa
     4561 agaaggactc tcatacgtat accttggaaa tgtgccaggt catccgcttg aacacactta
     4621 ctgttcacaa tgcaacaaca ttgtagtcaa aagatatggc tttgatattc aaggttggaa
     4681 catggatgag aataacaact gcaaattctg tggaaacaag attcctataa caggaaagct
     4741 tccaaaaaat tacaaacaaa gtagatttca gtttgtttat tagataaaaa tcaaatctaa
     4801 cactgatttt gatctgtcaa aggttaaata ttgtaaaacg aaaatgatct catgccaaca
     4861 acttacatcc tgctaaactc tgacttgggc tcagacgtag agatcatcaa aaaaatcaaa
     4921 gaaatactcg aaactgaggg caaagctgtc aagtatgaag tacaaggggt ttatggtgtt
     4981 tatgacatag ttgtcaaaat tacatcagat aacatggacc acctaaggaa cataattaca
     5041 aataaaatca gaaaaataga caaagtctat tccactctca caatgatggt aatagaagaa
     5101 caggaacaat aatctataat ttcttaattg cttcgacgac tttttgtatt tcagactctg
     5161 tattaaaaaa gtgcattgac gctctaatca cctttttact aaaaatttcc cttactgcaa
     5221 gaatcatgtt ttgtttttct agtctttcaa cgactgtttt tggatcttgg tcttgaatag
     5281 aaaatgatac tatactagtt cgcttctcag attcttctgg tccatatagc gtaatgcctg
     5341 ggattcttgc taactgctca cgtaaaatat ctgcaagttt gatatttttt tgcctgatgt
     5401 ttcctaatcc caaccgtaat agatagactg ctgatgcttc aagtcctgct actccagccc
     5461 agtttctaaa ccctgcctga aatttagatg gcatttcctt atgggtaatc ttattttcat
     5521 gcaaaaacgc agattctccg cctacctgaa gtggttctag taactcactt gattcttttt
     5581 tgcaaaaaaa tattccagtt cccattgggc cacaaagcca ttttgagcca ttaaatgaca
     5641 taaagttgca tttgatcttg tttacatcta cattctcaac gcaaccaaca gtttgagccg
     5701 agtctacaaa aaacggaatg ttatttgtag caagtaattc tccaactttt tcaacaggaa
     5761 gaattgctcc cgtgttaaat agtgcatggc ttaatgctac aagaccagta ttgttatcaa
     5821 ttgtttcttt aagatgttca aactcaaaat atcctgtttc attaacttgc aaatttctaa
     5881 tctcgcattt ttccgaaaga cgaatccatg gataatgatt tgctggatgt tcatgacttg
     5941 caccacgtgt tatgatgttt gatttttttt ctaattttaa tccatttgct acaatgttga
     6001 caccatcagt cgtactctga gttagaataa cttcttctgg attacagttt atcaaacggg
     6061 aaatggcttt tctagcaaga aaaagcttct ctctcacaaa attgtctgct gtactagaat
     6121 cgggccctaa ttcgttatat gtcacaagaa aatcagtcat tgcctttatg ctagaaatcg
     6181 gcatgagtga agttgaagca ttattcaagt agattctact tttaaatgga aaatcttgtg
     6241 cgacatcata atctgaattc attattatat tacaacatca ctacatgttt tgttgcttaa
     6301 aaatcatgat aatgatgtaa ccaaaaatgt tgttttagat ttagatgata tactttcaca
     6361 atccaaaatt aattctgtct tatactctga tccatttctg aaagcaggtt ttcttgcaaa
     6421 acttgtacaa gacacaaaga tcgatgtact ctatcttgat cttgatttat tgtatagtgg
     6481 ttatcttgca tctgaaatac ttggtacccc acaaaacctt gttttatttc agccaactct
     6541 tgaactatgg aatcagatgt taactgacgt gttagtaaaa ttaagtaata aaaaatctct
     6601 agttgtaatt gattcactta atggcctgta taatatttta aatgaaagaa aagaagttgg
     6661 caaacttgtt acttcatata taatgttaat caccagcata gcaaaaataa ctaattctta
     6721 catcgttgtg gcaagtatgg tcagatttaa aaaagaagaa ggttgggtac tatctcctac
     6781 aggaaagcgc cttattgaga caagtatggc aaaaaaaata cttttgggcc aaaatgattc
     6841 agaaataaca ctggattttc ttaaggagaa gaaaaaaatc accattccat taaatgcaat
     6901 tccgttagtc taatcgtttt attatatggt aaccaaact
//
LOCUS       DATIDL010000078         4193 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrosopumilaceae archaeon isolate SMAG_U16342
            k141_2922871, whole genome shotgun sequence.
ACCESSION   DATIDL010000078 DATIDL010000000
VERSION     DATIDL010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935836
            Sequence Read Archive: SRR25158326
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosopumilaceae archaeon (soil metagenome)
  ORGANISM  Nitrosopumilaceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrosopumilales;
            Nitrosopumilaceae.
REFERENCE   1  (bases 1 to 4193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/04/2024 05:09:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,520
            CDSs (total)                 :: 1,474
            Genes (coding)               :: 1,474
            CDSs (with protein)          :: 1,474
            Genes (RNA)                  :: 46
            rRNAs                        :: 1 (5S)
            complete rRNAs               :: 1 (5S)
            tRNAs                        :: 43
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4193
                     /organism="Nitrosopumilaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2922871"
                     /isolate="SMAG_U16342"
                     /isolation_source="soil"
                     /db_xref="taxon:2202732"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1067)
                     /locus_tag="VLB45_03375"
     CDS             complement(<1..1067)
                     /locus_tag="VLB45_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010190100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSD04776.1"
                     /translation="MSILTKGQKAIVVGSFLGWSLDGYDIVLMLLVIPSVSQLFFPSE
                     DPIFSLLAAFASYTATLIMRPLGAAIFGIYGDKFGRKKSMIITILGFSIATFVTGLLP
                     TYAMVGIFAPALLILTRMIQGVFAGGEWGSGAVITMESVPKQKRGILSGFLQSGFSFG
                     FLLASIAFQIITVSFPGDAFSEWGWRVLFFTGIIPGIIALFVRFRMDESALWLEKQQS
                     HRLEKTPLRKIVFGKEHRKAFFLCVFVMTGLVYMYHGSVGFLPTLLQQFGHIDRIHVA
                     PIMISATVSSWLGMILTGWLSQKIGRKKSMIFFAAGSIIFVIPLAYGILGEDLMATIL
                     FVIIYAFIVSTASGPVPAFFS"
     gene            1164..1844
                     /locus_tag="VLB45_03380"
     CDS             1164..1844
                     /locus_tag="VLB45_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HSD04777.1"
                     /translation="MSYIEQRFNYVTSRQIMPQWMSWLRSNEKEILAILNDLACKAEE
                     VSALLVDLFSNFEKLEENHAKIRNLEREADKLTHSVFEELNKTFITPLDREDISRIAS
                     KTDDILDYIEGISGRIINFRITTSPPYMLEMAKELAVATKEVNYMITRLNNVKADKSI
                     IDHCRKVSESEHRVDEIHRKAVGELFDTKDVITIIKMKEIYDALETTSDRCQDVADSI
                     EDIVLKYT"
     gene            1856..2842
                     /locus_tag="VLB45_03385"
     CDS             1856..2842
                     /locus_tag="VLB45_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007549895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HSD04778.1"
                     /translation="MYELAIGAILVALFFDFVNGFHDAANSIATVVGTRILRPLQAVT
                     LAAVANFVGPFVFGVAVATTVGKGIINPDFVTIYIVIGALVGAIIWDLITWLLGLPSS
                     SSHALVGGILGAGIAGAGMHAVILGGLEKVGIGIIVSPVVGLIGAFLLATLIITLFAK
                     KRPSKVNSVFGRLQLVSSTYFSLTHGANDGQKTMGIIALILLTEGVITSFEVPFYVIL
                     IAALAISLGTFFGGWRIVKTMAVKITQLKPYQGFAAETGGATVLAALAHFGIPASTTH
                     AISGAIMGAGAVKRVSAVRWGIGKRIVWAWIITIPASAAVAYVSTLIIHVFV"
     gene            3199..3486
                     /locus_tag="VLB45_03390"
     CDS             3199..3486
                     /locus_tag="VLB45_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016940139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HSD04779.1"
                     /translation="MSKQQYRSEMGIIADILGVTMDGGTQGVIVSAISRRANLSHYAV
                     LDKCQKLIDAGLVDSMKDNRNRLFVITEKGIRFFQEFQRFQNLVQSMNLRY"
     gene            3490..>4193
                     /locus_tag="VLB45_03395"
     CDS             3490..>4193
                     /locus_tag="VLB45_03395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04780.1"
                     /translation="MEQRLQLVHREYAPPEYATVKYTTQERMIFVRIVHTLSIMVRAP
                     IIAACFVMAALASLPYQSFGTTKVLDFTIYTDGTTHVFYQAEVDPLEPDFTVELFGNT
                     IDNFVAQDENDLLLASKIDTNSVVIETLGASEVKIDYDTADLVAKNGKIWSFQIDAPS
                     DYSLLLPKNTVIVGISTSPLNMQVVDEQSLISLPSGPVEINYVFGVLGTSQTATLAIE
                     KAQDLIDKINSEGIETP"
BASE COUNT         1356 a          796 c          814 g         1227 t
ORIGIN      
        1 tcagaaaaaa atgcaggcac tggtccagat gctgtagaaa ctatgaatgc ataaatgatt
       61 acaaagagaa ttgtagccat tagatcttct cctagtattc cataagctag tggaatcaca
      121 aagatgatag agccagcagc aaagaagatc atagattttt ttcgtcctat tttttggcta
      181 agccagccag ttaggatcat gccaagccaa gatgaaactg ttgcagaaat cattattggc
      241 gcaacatgta ttctgtctat atgcccaaat tgttgtaata gagttggcaa aaaaccaact
      301 gagccatgat acatgtaaac aagaccagtc attacaaaaa cacacaaaaa gaatgccttt
      361 cggtgttctt tgccaaagac aatctttctc agaggggttt tttctagtct atggctttgc
      421 tgtttttcca accaaagagc tgattcatcc attctaaatc taacaaaaag tgctatgatt
      481 cctggaatga tgcctgtaaa gaaaagcact cgccaacccc attcagaaaa tgcatcacca
      541 ggaaaactaa ctgttattat ctgaaatgca atcgaagcta aaagaaaccc gaaggaaaat
      601 ccactttgca gaaaacctga caaaattccc cgtttttgtt ttggtacact ttccattgtg
      661 atgacagcac cactacccca ttctcctccg gcaaaaactc cctgaatcat tcttgttaat
      721 atcaaaagag cgggagcaaa aattccaacc atagcataag ttggcaagag tcccgtaaca
      781 aaagttgcaa ttgaaaatcc caaaatagtt attatcatgg atttttttct tccaaactta
      841 tctccataga taccaaaaat tgctgcacca agtggtctca taattaatgt cgcagtatag
      901 cttgcaaagg ccgcaagtaa actgaaaata ggatcttctg atggaaagaa taattgactt
      961 acagatggta tcacaagaag catcagaact atatcgtatc catcaagcga ccagccaagg
     1021 aatgaaccaa ctacaattgc cttttggcct ttagttaaaa tggacatttt tctgaattaa
     1081 tacaaactca acttgtattc tagctttgtt ttgtttgtat tcatcaattc ttgattattt
     1141 tcttgactta ctatatagtt cctatgtcgt atatagaaca gagatttaac tatgtaactt
     1201 cgagacaaat catgccacaa tggatgtcat ggcttagatc taatgaaaaa gagatcttgg
     1261 ccattttaaa cgaccttgct tgtaaagctg aagaagtatc agctcttcta gtagatctat
     1321 tttcaaactt tgaaaaatta gaagaaaatc acgcaaagat cagaaatcta gagagggaag
     1381 cagacaaact aacacattct gtttttgaag aactaaacaa aactttcatc actccactag
     1441 acagggagga catctcaaga atagcatcaa aaactgatga tattttagat tatatcgaag
     1501 gaatatctgg tagaatcatc aactttagaa ttacaacatc tccaccatac atgcttgaaa
     1561 tggccaaaga acttgcagtg gcaacaaaag aagtcaacta catgattacg agattaaaca
     1621 acgtcaaggc agataaatcc ataattgacc attgtagaaa ggtcagtgaa tcagaacacc
     1681 gtgttgatga aattcatcgt aaagcagtag gagaattatt tgatacaaaa gatgtgatta
     1741 caattatcaa aatgaaagag atctatgatg cacttgaaac tacatctgat agatgtcaag
     1801 atgtcgcaga ctcgattgaa gacattgtac taaaatatac ttagggtttt ttgccatgta
     1861 cgaacttgcg ataggagcaa ttcttgtagc attgtttttt gattttgtta atggatttca
     1921 tgatgcggca aactctattg caactgtggt aggcacaaga attctaagac cacttcaagc
     1981 agttactctt gccgctgtag caaattttgt tggaccattt gtttttgggg tagctgttgc
     2041 aactacagta ggaaaaggaa ttatcaaccc agattttgtg acaatttaca ttgttattgg
     2101 agcattggtt ggtgcaatta tttgggattt aattacatgg ttactaggtc tgccatcttc
     2161 gagcagtcat gccttggtag gaggtatatt gggtgcaggt atagctggtg caggcatgca
     2221 tgcagtgatt ttaggtggat tagagaaagt cggtattggt attatagtgt ctccagtagt
     2281 tggtctcatc ggcgcatttc tactagcaac tctgatcatt actctatttg ctaaaaagag
     2341 accaagtaag gtaaattccg tctttggtag attacagctc gtttcatcta cctatttctc
     2401 tctaacacat ggagcaaatg atggtcaaaa aaccatggga ataattgctt taatcttact
     2461 aacagaaggc gtgattacca gttttgaagt gccattctac gttatcctta ttgcagcact
     2521 tgcaattagt cttggtacgt tttttggtgg ttggcgaatt gtcaaaacaa tggcagtaaa
     2581 aattacccag ttaaaaccat accaaggttt tgcggcagaa acagggggag ccacagttct
     2641 tgccgccctt gctcattttg gtatcccagc aagcacaaca catgcaattt ctggagcaat
     2701 catgggggca ggagcagtaa aacgtgtttc tgcagtaaga tggggaattg gtaaacgaat
     2761 cgtctgggct tggattataa ctataccagc cagtgccgca gtagcatacg ttagtacact
     2821 aatcatacat gtttttgttt aatcttgacg atccaatgtc tcattaactt caatgacact
     2881 tttgcagatg ggctggctac ccaaaaaggt cggagtcgtt tggtgcgtcg ggttttaatt
     2941 ccgtaatcgg ggaggtcaat tgaaggcctc catcttttta aaaaataagc gtggtttatt
     3001 ccatcaaagt cgtaaaataa gaattctaga tgaaatctgt tcatatttgg gcattcatca
     3061 tccaaagaaa ctctctgccc ccgccacacg aattacctat ttttgaccat ttcacacccc
     3121 taaacagccg ttccgctagg cgttccgctg tggtgtttca aggcgtccct gataatacat
     3181 ccgtcctata gtgatggcat gtcaaaacaa caatacagat cagaaatggg cataattgcc
     3241 gatattttgg gtgtgaccat ggacggtggt acacaagggg taatcgtgtc tgccatatct
     3301 agacgggcaa acctgtcgca ctatgcagtg cttgacaagt gccagaagct aatagacgcc
     3361 ggtttggtag attcgatgaa agacaacaga aaccgcctct ttgtgattac cgagaaagga
     3421 ataaggtttt tccaggaatt tcaaagattc caaaacctag tccagtcaat gaacctaagg
     3481 tattaaaaaa tggagcaaag actacagcta gtacatagag agtatgctcc gccagagtat
     3541 gctacagtaa agtataccac acaagaaaga atgatttttg taaggattgt ccataccctg
     3601 tcaataatgg tcagagcacc aatcatagca gcatgtttcg ttatggccgc cttggcatca
     3661 ttaccatacc aatcatttgg aactaccaaa gttcttgatt ttacaatcta taccgatggt
     3721 acaacacatg tattttatca agcggaggtt gatccactag aaccggattt tacagtagag
     3781 ttatttggta atactattga taattttgtg gcgcaggatg aaaatgatct tttactagct
     3841 agcaaaattg ataccaactc tgtagtaatt gaaactttag gtgcatctga agttaaaatt
     3901 gattatgaca cagctgatct tgtagcaaaa aatggcaaga tctggtcatt ccaaattgat
     3961 gccccttctg attattctct acttttacct aaaaatacag taattgttgg tataagcaca
     4021 tctccactca atatgcaagt tgttgatgaa caatcactta tttcattgcc aagtggtcct
     4081 gttgaaatta actatgtgtt tggtgtctta ggaacatctc agactgctac acttgctatt
     4141 gaaaaagcac aagatctaat tgataaaatt aattctgaag gaattgaaac acc
//
LOCUS       DATIDL010000079         5182 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrosopumilaceae archaeon isolate SMAG_U16342
            k141_703409, whole genome shotgun sequence.
ACCESSION   DATIDL010000079 DATIDL010000000
VERSION     DATIDL010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935836
            Sequence Read Archive: SRR25158326
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosopumilaceae archaeon (soil metagenome)
  ORGANISM  Nitrosopumilaceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrosopumilales;
            Nitrosopumilaceae.
REFERENCE   1  (bases 1 to 5182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/04/2024 05:09:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,520
            CDSs (total)                 :: 1,474
            Genes (coding)               :: 1,474
            CDSs (with protein)          :: 1,474
            Genes (RNA)                  :: 46
            rRNAs                        :: 1 (5S)
            complete rRNAs               :: 1 (5S)
            tRNAs                        :: 43
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5182
                     /organism="Nitrosopumilaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_703409"
                     /isolate="SMAG_U16342"
                     /isolation_source="soil"
                     /db_xref="taxon:2202732"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(47..1096)
                     /locus_tag="VLB45_03400"
     CDS             complement(47..1096)
                     /locus_tag="VLB45_03400"
                     /inference="COORDINATES: protein motif:HMM:TIGR01728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HSD04781.1"
                     /translation="MNPSIKFGIAAAIIVAVIISSYSGMQLSGNNNSGTADTIRIGYF
                     PNINHAQAVIGLGNDNFQKTLGDVKIETQIFNAGPSAIESLLANRVDVTYVGPNPAIN
                     GYIKSEGEALRIISGAASGGAVFVVRNDAGINSVNDFADKKFASPQLGNTQDVALRSY
                     LLKNGFKTTENGGNVEVIPAKIPDILTLMLKNEIDGAWIPEPWGAKLVKETNSRIFLD
                     ERDLWSEGKFVTAHIIVNTEYLKNHPDTIKKLLEAHVDETIWINEHKEESIKDFNFEL
                     EKLTGKTIPEDEIRDAFSRLEITYDPAKESLFKSADNAFEIGFLGETRPDLSEIYDLS
                     ILNEILKERNLPIVN"
     gene            complement(1155..1961)
                     /locus_tag="VLB45_03405"
     CDS             complement(1155..1961)
                     /locus_tag="VLB45_03405"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSD04782.1"
                     /translation="MLVHEESEISHKRRIDLHDAANAVLFLAAFVGIWQIVFMLGIWP
                     EVALPSPATVAESFARIILNHSLLNGIVITMARLVAGFAIAIGLGAAIGLAMIKFKGF
                     GKTMQSFSAGLLSFPSIAWVPFSILLIGFNDAGILFVVIMSSVFSITISTYSSIRNIP
                     TMYIDAAKNMGAHGLSLFLHVTIPAATPSLIIGIRQGWSFAWHAIIGAEILMSIVGLG
                     HILSVGREFQDMGQVIASMITIFTIGLLVDRLLLFKLEEKVRSRWGLNRR"
     gene            2082..2333
                     /locus_tag="VLB45_03410"
     CDS             2082..2333
                     /locus_tag="VLB45_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04783.1"
                     /translation="MEDSEHDSRIEKVASLIAKDKTSLDEQNPINLQKYYDFVKTNFD
                     LDGESAVQLVNEAFLYLKLKNSDSIDLLQEGDKFGAGFS"
     gene            2365..3312
                     /locus_tag="VLB45_03415"
     CDS             2365..3312
                     /locus_tag="VLB45_03415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04784.1"
                     /translation="MKYLFIFLIGFSVFVVCNVHAETIPSWIKQTAGLWAEKKIDDSK
                     FIQSLEHLTKDGILQIKIEHDSTNVYILPKYGQTSLVTISGTTGDFKKRNNVILIILQ
                     PDGKTIDLTAAVLESGIYQTTLVLNHDSPVGLYTITGMFSGSPIQESSFYVKQSSSPK
                     IPSWIKNNARWWADGKISDNDFVLGIQYLIDSKIMIVDFQNPNMQNHLQIYVQGKEQV
                     RRGTMQSINVHVTDGENPLSGATVQIRVEDYGENLFKEFQGITDSKGEYFFSWEIDKN
                     ADAETLLVFVDVTDGFSSTSQMFSFQVICLCGEPNCECR"
     gene            3375..3830
                     /gene="msrA"
                     /locus_tag="VLB45_03420"
     CDS             3375..3830
                     /gene="msrA"
                     /locus_tag="VLB45_03420"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010195211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HSD04785.1"
                     /translation="MKATFAAGCFWCVEPVFRKVKGVTSTAVGYIGGHAENPTYEDVC
                     NGQTGHAEAIQIEYDPSVVPYEELLNVFWENHDPTSLNRQGPDVGEQYRSAIFFHSPE
                     QEATARASKERQQNSGIYTKKIVTEITPATKFYKAEEYHQRYYEKCGLA"
     gene            3926..4843
                     /locus_tag="VLB45_03425"
     CDS             3926..4843
                     /locus_tag="VLB45_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription factor TFIIB"
                     /protein_id="HSD04786.1"
                     /translation="MIFSRNAKDHSQDSTTHGPVITDNVSGEILCGRCGLVLVEKVED
                     PGLESIGYTTDQYLEKSRTGMKSTLALHDRGLATMIDAKDKDAHGNTLSSYVKDTFGR
                     LRMWDTRSKFQNDDRSLRSAFVVLDTIKQKLELPENITERAAYLYRKALAKKLTRGRR
                     ITDLMLASIYTACREANTPRTLQDVANAGNTSIKGLSRHYRILVESLDLHIQSYDSSD
                     FVTRISEAVGLSEKTRRSVLEILTKVKQKEMSAGKNPMALAAAAIYLASVMNMEGKSQ
                     KNLSKVSGISMVTIRERARAIKKSLGKEI"
     gene            4931..>5182
                     /locus_tag="VLB45_03430"
     CDS             4931..>5182
                     /locus_tag="VLB45_03430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04787.1"
                     /translation="MKSLNKFLILGFTLAMLIVTPYLNVSAMEMKNSMMGKLTNTSVP
                     VTIPLTKGYAKGSEVFYISTEASDKDLADHLTKLTGFRVA"
BASE COUNT         1727 a          943 c          964 g         1548 t
ORIGIN      
        1 atgatgtact tgataagaaa tagatttttc aagtctcttt aaatcatcag ttcacgattg
       61 gaagatttct ctctttgaga atctcgttta ggatgctcaa atcataaatt tctgacaagt
      121 ctggtcttgt ttcacccaaa aatcctatct caaatgcatt atcagctgat ttgaaaagcg
      181 attcttttgc cggatcatac gtaatttcta acctggaaaa tgcatctcgt atctcatctt
      241 caggaattgt ctttcctgtg agtttttcaa gttcaaaatt aaaatctttg atggattctt
      301 ctttatgctc atttatccat attgtttcat ctacatgtgc ttcaagtaat ttttttattg
      361 tatcgggatg attcttgaga tattcagtat ttacaatgat gtgagctgtt acaaattttc
      421 cttctgacca taaatcgcgt tcatcaagaa atattctact gtttgtttcc ttgactagtt
      481 ttgcccccca tggctctgga atccatgccc catcaatttc attttttagc atcaaagtta
      541 gaatatcggg aatctttgct ggaatgactt caacattacc accattttca gtagtcttga
      601 atccattttt tagtaaatat gatctaaggg ccacatcttg agtattgcca agttgtggcg
      661 atgcaaattt tttatctgca aaatcattaa ctgaattaat accagcatcg tttctaacta
      721 caaaaactgc tcctccactt gcagcaccag aaataatccg tagtgcctcg ccttcagatt
      781 taatatatcc gttaattgca gggtttggtc caacatatgt tacatcgact ctatttgcaa
      841 gtaatgattc tattgcagaa ggaccagcat tgaatatctg tgtctcgatt ttaacatcac
      901 ccagagtttt ttggaaattg tcatttccaa gtccaattac agcttgggcg tggtttatgt
      961 ttggaaagta accgattctt atagtatctg cagttccact gttgttatta cctgataact
     1021 gcatgcctga atacgatgaa attattactg ctacaattat tgcagccgca attccaaatt
     1081 ttatactagg attcaattga ttttgatgac agaatccatg ttaaattcat ttacaaaatt
     1141 agttcaaact aattctatct acgattcaga ccccatcgtg atctgacctt ctcttctaac
     1201 ttgaacaaca agagcctgtc tactagtaaa ccaattgtaa agatggtaat catgctggca
     1261 atcacttgac ccatgtcttg aaattctctt cctacagata gaatgtgacc tagtccaact
     1321 attgacatta gtatttcagc accaattatt gcgtgccatg caaatgacca tccttgtctt
     1381 attcctatga tcagagatgg tgtcgcagct ggtattgtga catgcaagaa aagtgacaaa
     1441 ccatgagctc ccatattttt tgccgcatct atgtacatgg taggaatgtt tctgatactg
     1501 ctgtatgttg atattgtaat tgaaaaaact gagctcatta ttacaacaaa tagtattcca
     1561 gcatcattaa agccaattaa aagaattgaa aacggaaccc acgcgatgct tggaaatgat
     1621 aatagtccag ctgaaaacga ctgcattgtt ttcccaaacc ctttgaattt tatcattgct
     1681 agtcctattg cagctcctaa accaatggca atagcaaatc cagcgacaag tcttgccatg
     1741 gttatgacta ttccatttag taacgaatga ttcaaaatta ttctggcaaa agattcagcc
     1801 acagttgcag gagaaggcag tgccacttca ggccatattc caagcataaa tactatctgc
     1861 caaatgccga caaatgctgc tagaaataat actgcattag ctgcatcatg cagatcaatt
     1921 ctgcgcttat gtgaaatttc tgattcttca tgtactagca tactgtccaa tcctattgat
     1981 taggtcagta tatttattcg cagatggttg cgttactaga tagtgtgaga caagcatttt
     2041 gtggaacatc tttcaaatag tggacttgat catacaaact attggaagat tctgaacatg
     2101 attctcgaat tgaaaaggta gcatcattaa ttgcaaaaga caaaacttca cttgatgaac
     2161 aaaatccaat aaacttgcaa aaatattatg attttgtaaa aacaaacttt gacttagatg
     2221 gtgaatctgc ggtacagctt gtcaatgaag catttttgta cctgaaacta aaaaactctg
     2281 actcgataga tctattgcaa gaaggagaca agtttggagc aggatttagc taaaaatgaa
     2341 atctgtctat tgataaaaaa gtctgtgaaa tatctgttta tatttttgat tggtttttct
     2401 gtatttgtag tatgtaatgt gcatgctgaa accataccgt cttggataaa acagacggca
     2461 ggtttgtggg ctgagaaaaa aattgatgac tctaaattta tccaatcatt agagcatctt
     2521 acaaaagacg gaatactgca aatcaaaata gaacatgatt ctacaaatgt atacatacta
     2581 ccaaaatatg gtcaaacatc acttgtcaca atttctggaa ctacaggcga tttcaaaaaa
     2641 agaaacaatg tgattcttat catactgcaa cctgacggca aaacaattga tctgacagcc
     2701 gctgtcttgg aaagcggtat ctatcagacc actttggttt taaatcatga ttctccagta
     2761 gggctctaca caattactgg catgtttagc ggatctccta tccaagaatc tagtttttat
     2821 gtaaagcaaa gctcttctcc aaaaatccct tcatggataa aaaataatgc gcgctggtgg
     2881 gcagatggaa aaatatctga taatgacttt gttttgggaa tccagtatct gattgattca
     2941 aaaatcatga ttgtagattt tcaaaatcca aacatgcaga atcatctgca aatctacgta
     3001 cagggaaaag agcaagtaag gcggggcaca atgcaatcaa ttaatgttca tgtgacagat
     3061 ggagaaaatc cactaagcgg tgctactgta caaatcaggg tggaagatta tggggaaaac
     3121 ctattcaaag aattccaggg aataactgat tctaaaggtg agtatttttt ctcatgggag
     3181 attgataaaa atgctgacgc tgaaacgctt ttggtatttg tagatgtaac cgatggattc
     3241 tcttcaacta gccagatgtt ttcatttcaa gtaatttgtc tttgcggaga accaaactgt
     3301 gaatgtcgat gatgcttatt tttcatacta aagattagct agggtttaat ttagccaaat
     3361 ttgcaaaaag actcatgaaa gccacgtttg ctgcagggtg tttttggtgc gttgaacctg
     3421 tatttagaaa agtaaaaggc gttacatcaa cagcagtggg atatatcggt ggacatgccg
     3481 aaaatccaac atatgaggat gtctgtaacg gacagacagg acatgcagaa gcaatccaga
     3541 tagaatatga tccatccgtg gttccatatg aggaactttt gaacgtgttt tgggaaaacc
     3601 atgatccaac atcacttaat agacaagggc cagacgttgg agaacagtat cgatctgcca
     3661 tcttttttca cagtccagaa caagaagcta ctgcaagggc atcaaaggaa aggcaacaaa
     3721 actctggaat ctatacaaaa aaaattgtta cagaaataac acctgctacc aagttctaca
     3781 aggcagaaga ataccatcaa agatactatg aaaaatgtgg actggcttga tgtgatagtt
     3841 ttttgcaact aaccatcagg taacttactt tctttgccga caccatcaca atttaagtac
     3901 ttacctttgt atagtaagta taagaatgat atttagccgc aatgcaaaag accatagtca
     3961 agacagtaca acacacggtc cagtcataac tgacaacgtt agcggagaaa tcctttgtgg
     4021 aaggtgtggt cttgtcttgg ttgaaaaagt tgaggatcct ggacttgaat ctataggata
     4081 caccacagat caatacttgg aaaaaagtag aactggaatg aagtcaacac tggcgttgca
     4141 tgacagagga ttggcaacta tgatagacgc caaagacaag gatgcacatg gaaatacact
     4201 gtcaagctat gtcaaagaca catttggaag actcagaatg tgggacacta gaagtaaatt
     4261 tcaaaatgat gatcgaagct tgagatcagc atttgtagta cttgatacta taaagcaaaa
     4321 gcttgaactc ccagaaaaca ttacagaacg tgctgcatat ctttacagaa aagcgttagc
     4381 taaaaaatta acacgcggta gaagaattac agacttgatg cttgcatcaa tttatactgc
     4441 ttgcagagaa gcaaatactc caagaacttt acaggatgta gcaaacgcag gaaacaccag
     4501 catcaaagga ctttcaagac attaccgaat actagttgaa tcacttgatt tacatattca
     4561 atcatatgac tcgtcagatt ttgtcacaag aatatctgaa gcagtaggtc ttagcgaaaa
     4621 aaccaggaga agtgtactag aaattctaac caaagtaaaa caaaaagaga tgtctgcagg
     4681 aaaaaacccg atggcacttg cggctgcagc catatacttg gcatctgtaa tgaacatgga
     4741 agggaaaagt caaaagaacc tctccaaagt ctctggaata agtatggtga caatacgaga
     4801 gagagcaaga gccattaaga aaagtctagg aaaagaaatc tgatagattc gtttttttat
     4861 tttgtgaaat tgaggaattg gatcagatct ggaaaaaatg ttaaatgcta aaagataaat
     4921 tcattcgtaa atgaaaagtc taaacaaatt tctgattctt ggctttacac ttgccatgtt
     4981 gattgtaacc ccatatctga atgttagtgc catggaaatg aagaattcta tgatgggcaa
     5041 actgactaac actagcgtac cagtgacaat tccattaacc aaaggatacg ctaaaggttc
     5101 tgaggttttt tacatatcca ctgaagcatc tgataaagat cttgcagatc atttaacaaa
     5161 acttacaggt tttagggttg ca
//
LOCUS       DATIDL010000080        11226 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrosopumilaceae archaeon isolate SMAG_U16342
            k141_2116973, whole genome shotgun sequence.
ACCESSION   DATIDL010000080 DATIDL010000000
VERSION     DATIDL010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935836
            Sequence Read Archive: SRR25158326
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosopumilaceae archaeon (soil metagenome)
  ORGANISM  Nitrosopumilaceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrosopumilales;
            Nitrosopumilaceae.
REFERENCE   1  (bases 1 to 11226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/04/2024 05:09:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,520
            CDSs (total)                 :: 1,474
            Genes (coding)               :: 1,474
            CDSs (with protein)          :: 1,474
            Genes (RNA)                  :: 46
            rRNAs                        :: 1 (5S)
            complete rRNAs               :: 1 (5S)
            tRNAs                        :: 43
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11226
                     /organism="Nitrosopumilaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2116973"
                     /isolate="SMAG_U16342"
                     /isolation_source="soil"
                     /db_xref="taxon:2202732"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..304
                     /locus_tag="VLB45_03435"
     CDS             <1..304
                     /locus_tag="VLB45_03435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="5,10-methenyltetrahydrofolate synthetase"
                     /protein_id="HSD04788.1"
                     /translation="KIHKKIEAQPKGFVTQVISSVDQVTRIVDKLKPQGFKIVPCVLL
                     SSEKNSKSAKMLNLDWSSYSNDVVGFINKVNKIAGNVLVSSPSDFSGALEVLKKLV"
     gene            367..1446
                     /locus_tag="VLB45_03440"
     CDS             367..1446
                     /locus_tag="VLB45_03440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HSD04789.1"
                     /translation="MKNSVIIQASFVVFVLVLMIQYANAEVWIPENEFSSYFDENGIY
                     TVIGAVKNSESYPIVPTVELRIQDGNKIISESYTLPVANPLKDIPFKIKFDQISSKNP
                     ILEKPAVTFVPGPTDYMNIEIVYDDTLVRHKDGHTSGFIVNNGNSTAYGVKVYALIHG
                     AVSKFIDVGKSVETIEKIEPGEKKEFSIYPDPLLATQVNFYSCFVIGEDMVIPTYVMR
                     DDTRFDFRYKTEGYLNDIKFDDEKNALTFLVRNPWPMESFVNIEFPMESDSQKFVVYQ
                     EGKEIKSIQSRDEENTWHVAFNLEPRANEQIVISGFESNKEPQNVFNGEMRPQSAFDA
                     YYLLGIIPAAAIIALVFKARKKKPL"
     gene            complement(1449..2228)
                     /locus_tag="VLB45_03445"
     CDS             complement(1449..2228)
                     /locus_tag="VLB45_03445"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSD04790.1"
                     /translation="MTKLEAKNIVKQFDHNGNSVLALDGIDLKIEEGDFVCIVGPSGC
                     GKSTFLNIVAGLEKPDSGEILLNGKEITTPGPDRTMVFQEGALFPWLKVIDNVEFGLK
                     MAGIPKDECRQISQRYLDMMQLTKFADSYIYQLSTGMKQRVAIARALVMDPEILLMDE
                     PFAALDAQTRDLLLVELQLIWERTKKTILFVTHSISESVILGNRVVVFKNRPSKIKKE
                     FTIDYRRPRLAEDENLLKYQQDILAELRTEVKAQMKGELGQ"
     gene            2468..3655
                     /locus_tag="VLB45_03450"
     CDS             2468..3655
                     /locus_tag="VLB45_03450"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HSD04791.1"
                     /translation="MKGKAVLAFSGGLDTSVVVKYLQKEHNLDVVTVTVDVGQGDDQK
                     KIAAKAKKLGVVKHYNLDARDEFVKDFIFPSIKANALYQKKYCLATALARPLIAQKVV
                     EIAKKENAKALAHGCTGKGNDQVRFDVTMRSGSTLPIIAPIRDLNLTRDVELKFAKKH
                     GIQIDTAAKKFSIDQNLWGRAIEGGVLENAYNEPPDDAFIWVKTKNLPDKPQYLEIKF
                     ENGIPVAADGKRLKPIELIEYINKKAGSCGVGIVDHIEDRVVGIKSREVYETPAATCI
                     IEAHTDLEKMVLTKHELKFKSMVDSEWAWLAYSGLWQDPLKSDLDMFINATQMRVNGT
                     VKLKMFKGSLRVVGRKSDYSLYSHDLATYGSGSTFDQTLAKGFVELWGMQSTEANKLL
                     KKR"
     gene            3667..3834
                     /gene="lysW/argW"
                     /locus_tag="VLB45_03455"
     CDS             3667..3834
                     /gene="lysW/argW"
                     /locus_tag="VLB45_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019878 - lysine biosynthetic process via
                     aminoadipic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-aminoadipate/glutamate carrier protein
                     LysW/ArgW"
                     /protein_id="HSD04792.1"
                     /translation="MNCPECDAKLNIPDDATVGEIVSCPDCGADFEIASKNGSTCQLK
                     HAEKVGEDWGE"
     gene            3835..4692
                     /gene="lysX"
                     /locus_tag="VLB45_03460"
     CDS             3835..4692
                     /gene="lysX"
                     /locus_tag="VLB45_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine biosynthesis protein LysX"
                     /protein_id="HSD04793.1"
                     /translation="MSKLRIVFDRVRSEEKMLEEKAIELGHDTKMIDAKMTQINTESK
                     KSDFDFGDVVLERCVSYFRGLHFTACLEFLDVPVINKFEVANNCGNKLITSLLLKKHN
                     VPTPRTYFTFSSETALENLEKVGYPMVIKPVVGSWGRGVMALKDRDTADAIIEVRELN
                     DGPLDRIYYLQEMVKRPPRDIRVIAVGDHVVAAMYRTSTGGFKTNIALGAEPVPCEIT
                     KDLEDICLKASKAVGGGILGIDLMEDEKRGLVVHEVNNTVEFKGLAKVAKKNIPKEMI
                     DFAIKSAIR"
     gene            4723..5775
                     /gene="argC"
                     /locus_tag="VLB45_03465"
     CDS             4723..5775
                     /gene="argC"
                     /locus_tag="VLB45_03465"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013482452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HSD04794.1"
                     /translation="MRVGVIGASGYVGGEMLRLLVSHPKVEIAMVTSRQYVGEYVSRI
                     QPSLKSFIDITFSELDYDKLSDKCDIVFTAVPHGTATEIVKALYDRGMKVIDLSADYR
                     LHNPADYDKWYGWQHPHPELLSKSVFGVPELHREEIKKAQLVSCPGCMAVTSLLALYP
                     LVKRGMIDTEHIIVDSKIGSSGAGAGSVAGTHHAMRSGVIRPYKPAKHRHTGEIEQEL
                     SIAAGKKIKVSMSPHAVDIVRGILCTNHTFLTRPTTEMELWKMYREQYQNERFIRLIR
                     DKKGLYKFPDPKFVVGSNFCDVGFDIDEDNNRLVVLSASDNLMKGAAGSAIQNMNVMA
                     GYDEMDGLHYSPLTPV"
     gene            5779..6579
                     /locus_tag="VLB45_03470"
     CDS             5779..6579
                     /locus_tag="VLB45_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[LysW]-aminoadipate/[LysW]-glutamate kinase"
                     /protein_id="HSD04795.1"
                     /translation="MITIKIGGSVVDGLHPSTISDLKKVSQKEKIILVHGGGKEVTKI
                     SEALGKEQKFIVSPGGIKSRYTDKETVEIFTMVMSGKINKMIVGMLQKNGINAVGLSG
                     VDGQIIQAERKKKLVVINEKGRKMVIDGGYTGKIKDVNTSLIKSILDQGYVPVISPIA
                     ISEEFDFLNVDGDRAAAYVAGKMQSDKILFLTNVDGLLMNDKLVKNLSLAEAKEMLPK
                     IGFGMEKKVLAATEALEMGVKQALISNGQRENPISVAIANDNCTVITK"
     gene            6576..7751
                     /locus_tag="VLB45_03475"
     CDS             6576..7751
                     /locus_tag="VLB45_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HSD04796.1"
                     /translation="MTEDQFMGNIYQRFPVTVERGLGSHVWDINNKEYIDCMGGYGVA
                     LVGHRNPRVVKALQAQLEKIITVHSSLYNKTREEFLESLIKIAPKNLSQVYLNNSGTE
                     AVEAAIKFARKFTGKSGMIAMNGSYHGKSFGSLSLTFNPKYRKSFEPLVEKVSFSPFG
                     DIESLKSSVNSDTAFIIIEPIQGESGIHVAPDGFLQDVRKLCDEKGILLIFDEIQAGL
                     GRTGKMWAGQHWDTTPDIMCLAKGIAGGVPMGATLVRPDILACISKGEQSSTFGGNPL
                     SCAAGIATIQALTEDKLVENAEKMGKKFREGLESLKERHKVIREVRGKGLMIGVEMKF
                     EVKDILFDGIANGLLLLYSGKNILRLLPPLVTSEADITKALEILDVLLEKEEKRRNV"
     gene            7744..8163
                     /gene="lysM"
                     /locus_tag="VLB45_03480"
     CDS             7744..8163
                     /gene="lysM"
                     /locus_tag="VLB45_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007551137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0019878 - lysine biosynthetic process via
                     aminoadipic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator LysM"
                     /protein_id="HSD04797.1"
                     /translation="MSRDKTDETIIEMLKKNSRESFVEIGQKLGLSESAVRRRVKNLT
                     DGGIIKKFTIETGDANATSAIILISVDSATDTSKVSAKLTKLQGVHTVYEITGQYDIA
                     VIIRAPTITDINVCIDSLRKVPGVFDTNTVIILRTVA"
     gene            8281..9471
                     /locus_tag="VLB45_03485"
     CDS             8281..9471
                     /locus_tag="VLB45_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008297892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HSD04798.1"
                     /translation="MDDPNYYAYQYNAMGQKPKKIHVLDSTLREGEQHPGVTFSNKQR
                     IQIAWMLDYFGVDQIEISPVVSNDHKEATKTIIKQGLRADIVAHVRALKEDVDVAIDC
                     DAVWMATYLGMSDIHLKDKLRISREEAMRRAVETVEYAKSHGLKMRFTMEDASRAEPE
                     FLIKMCKAIEDAGVDRISIPDTVGIMRPIGMYNLVKTVRDQIKIPLDVHCHNDIGFAL
                     ANAFAGCDAGADQIHTTIDGIGERTGIPALAETAVALTYLFKSPNNFRLDMLMDLSRL
                     IEQYTTIKPYDSKPIVGSTAYKHKAGTHLAAILRNPAAYEPIEPKVVGNRRRIVFGEL
                     AGKNGAAYLMSLLGLDQNDEHAKAVAAGLKNLRMGDLLEIPLDDRLERKIINDTQIKV
                     KPGK"
     gene            9473..9637
                     /gene="lysW/argW"
                     /locus_tag="VLB45_03490"
     CDS             9473..9637
                     /gene="lysW/argW"
                     /locus_tag="VLB45_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019878 - lysine biosynthetic process via
                     aminoadipic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-aminoadipate/glutamate carrier protein
                     LysW/ArgW"
                     /protein_id="HSD04799.1"
                     /translation="MKCLECDATISPPGDAIEGEIVTCPDCGASFELAKGSTGFELKP
                     AQTVGEDWGQ"
     gene            9634..10482
                     /gene="lysX"
                     /locus_tag="VLB45_03495"
     CDS             9634..10482
                     /gene="lysX"
                     /locus_tag="VLB45_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine biosynthesis protein LysX"
                     /protein_id="HSD04800.1"
                     /translation="MSSGLSILYDTIRWEEKALLDAGKKKGIDIEMVDCKNLFLNLGK
                     TKEKFDTVIQRCVSYYRSLHSTAALEGKGINVINSLNTSIFAGNKLFTHMLLQKFGVP
                     TPFSTVAFSEEAALEALKENGYPMVLKPTVGSWGRMIALLKDQDSAEGIMESRERMYP
                     IYQVYYLEEFVQRPPRDIRAIMVGDKVVAAIYRYSGDGQWKTNMALGGKAEECKVTKE
                     LEDICVKAKNAVQGQIVGVDLMESKEKGLVVHEVNNTTEYKNTVRVTGVDIPALMIDY
                     ALQSRK"
     gene            10486..>11226
                     /locus_tag="VLB45_03500"
     CDS             10486..>11226
                     /locus_tag="VLB45_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HSD04801.1"
                     /translation="MDSSITITPRFSVKMLEKALRLYTPSLAEKPLAEFLADKCDDLG
                     FDNIHIDEVGNLIATKGSGSPKILLCGHMDTVPGKIKVKNDNGFLYGRGASDAKAPLM
                     AMLLSAASMQSNSGTVVFAAVVDEEGNATGIKSLVKQKLDIDYAIFGEPSGIRNITIA
                     YKGRIAINLKVNVGNSAHASAPWLAKNAIEEAYVFINALKKSLEGGQENKPKGMLLTA
                     TLTEIKGGTSHNVTAIECDAIMDIRIPVD"
BASE COUNT         3873 a         1992 c         2469 g         2892 t
ORIGIN      
        1 taaaattcac aaaaaaatcg aggcgcaacc aaaaggattt gtaactcagg ttatcagttc
       61 agtagatcag gtaacaagaa tagtggacaa attaaaacca cagggattca aaatagttcc
      121 atgtgtttta ttgtcttcag aaaagaattc aaaatctgca aagatgttaa atcttgattg
      181 gtcaagttat agcaatgatg ttgttggctt cataaacaaa gtgaataaaa ttgcaggaaa
      241 tgttcttgtt agttcaccta gtgactttag cggtgcgcta gaagtattaa aaaaactagt
      301 ttgatttcat tatagaaaac gtcctctaca ttaaaattcg gcaaactgtt cttacctgtt
      361 gtcttcatga aaaacagtgt catcatacaa gcaagttttg ttgtttttgt attagtatta
      421 atgatacaat atgctaatgc agaagtttgg attcctgaaa atgaatttag ttcgtatttt
      481 gatgagaatg gcatttatac ggttattggg gctgtaaaaa attcagagag ctacccaata
      541 gttccaaccg tagaactcag gattcaagac gggaacaaaa tcatatcaga gtcatacaca
      601 ttaccagttg caaacccact caaagatatc ccatttaaga tcaaatttga tcaaatctca
      661 agcaaaaatc caattcttga aaaaccagca gtcacatttg ttccaggtcc tacagattac
      721 atgaatatag aaatagttta tgacgacact cttgtcagac ataaagatgg tcacacttct
      781 ggtttcatag taaataacgg aaattctact gcgtatggag ttaaagtgta tgctttgatt
      841 catggtgcgg tgagtaaatt tatcgacgtt ggaaaaagcg ttgagaccat agaaaaaatt
      901 gaaccaggag agaaaaagga attttctatt tatccagatc cattgcttgc aacgcaagta
      961 aatttctaca gttgttttgt gattggcgag gatatggtaa taccaactta tgtcatgcga
     1021 gatgatacca gatttgattt tagatataag acagaaggat atctcaatga cataaaattt
     1081 gatgatgaaa aaaatgcgtt aacatttttg gtgagaaatc catggccgat ggaatctttt
     1141 gtaaacatcg agttcccgat ggaatcagat tcacagaaat ttgttgtata tcaagaaggt
     1201 aaagaaatta aatcaataca aagtagagac gaagaaaaca catggcatgt tgcctttaat
     1261 ttagaaccac gggccaatga acagattgta atttctggat ttgaaagcaa caaggagcca
     1321 caaaatgtgt ttaatggtga gatgagacca caaagcgcgt ttgatgcgta ttatttactt
     1381 ggcatcatac ctgcggcagc tataattgca ctagttttca aggcacgaaa gaagaaacca
     1441 ctgtaaattc attgtccaag ttcccctttc atctgtgctt ttacttcggt tctcaattca
     1501 gctagtatgt cttgttgata ttttaacaga ttctcatctt ctgcaagtcg tggtctgcga
     1561 tagtctattg taaattcttt tttgatttta gacggtctat ttttgaatac aactactctg
     1621 tttccaagta ttacggattc tgaaatgctg tgtgttacaa acaagatcgt cttttttgtt
     1681 cgctcccata tcagttgcag ttccaccaac agcaagtccc ttgtttgtgc atcaagtgca
     1741 gcaaatggtt cgtccattaa gagaatttca ggatccatga caagagcgcg tgcaattgca
     1801 actctttgtt tcatgccagt tgataattgg tatatgtatg aatctgcaaa ctttgtcagc
     1861 tgcatcatgt ccagatagcg ttgcgatatc tgtctgcatt catcttttgg aattcctgcc
     1921 atttttagtc caaattccac attatctatt acctttaacc aaggaaacaa tgcaccttcc
     1981 tgaaatacca ttgttctgtc tggaccagga gttgttatct cctttccatt tagcaaaatt
     2041 tcgccagagt ctggtttttc tagaccagct acaatgttta gaaaagtaga cttgccacag
     2101 cctgacggtc caactataca aacaaaatct ccctcttcta ttttcagatc tatcccatca
     2161 agtgccaaaa cagaattgcc attatgatca aattgcttta caatgttttt tgcttctaat
     2221 tttgtcatgt tatcaaataa acccccaatt tatcaaaagt tgtgtgtgat ttgccgattt
     2281 catatcatat cccgcagtga cgattttcat ataacagcgt tataatattg cggttaaata
     2341 gtgatcgaaa tttagctcaa gctatctgga ttttacaaac tcattttctg attttccatg
     2401 attagaaaac aaaaatcacg atcaatcaca aaagataaat gcgaaaatta aatcacagaa
     2461 aacctcaatg aaaggaaaag cagttttagc tttctctggc ggattagata catcggttgt
     2521 tgtcaaatat ttacagaaag aacacaactt ggatgttgtc acagtcacgg tagacgttgg
     2581 acaaggtgat gatcaaaaga aaattgcagc caaggcaaaa aagcttggag ttgtgaaaca
     2641 ttacaattta gatgcacgtg atgagtttgt caaagatttt atttttccat caatcaaagc
     2701 aaacgcactt tatcaaaaaa agtattgcct tgcaactgca ctagcacgac cactgattgc
     2761 acaaaaagta gtcgagattg caaaaaaaga aaatgcaaaa gcattagcac atggatgcac
     2821 aggaaaagga aacgatcaag tcaggtttga cgttacaatg cgatcaggtt ctactcttcc
     2881 aattatcgcg ccaatcaggg atctcaacct tacacgtgat gtggaattaa aatttgcaaa
     2941 aaaacatgga atacagatag acactgctgc aaaaaaattt agtatcgatc aaaacttgtg
     3001 gggacgtgcg atcgaaggcg gcgtattgga aaatgcatac aatgaaccac cagatgatgc
     3061 tttcatatgg gtaaagacaa aaaatttgcc agacaaacca cagtatttag aaataaaatt
     3121 tgaaaatgga atcccagttg cagctgatgg aaagaggcta aagccgattg aactaataga
     3181 atacatcaac aaaaaggcag gctcatgcgg agtcggcata gtggaccaca tagaagacag
     3241 ggttgtaggc ataaagtctc gtgaggttta cgagacacca gcggcaacct gtatcataga
     3301 ggcccacact gaccttgaaa agatggtact cacaaagcat gagctcaaat tcaaatcaat
     3361 ggttgattca gaatgggcct ggttggcata ttctggatta tggcaagatc ccctaaaatc
     3421 agatttagat atgtttatca atgccacaca aatgagagtt aacggaactg taaagcttaa
     3481 gatgtttaaa ggaagtctac gagttgttgg aaggaaatca gattattcat tgtatagtca
     3541 tgacttggca acatatggat ctggttcgac ttttgatcaa acccttgcaa agggatttgt
     3601 agaattgtgg ggaatgcaat ccacagaagc caataaattg ctaaaaaaga ggtgaaagag
     3661 taaaaaatga attgtccaga atgtgacgca aaattaaata tcccagacga tgcaacagtc
     3721 ggagaaatag tttcgtgccc tgattgtgga gcagattttg agatcgcatc aaaaaatggt
     3781 tccacatgcc aactaaaaca tgctgaaaaa gtcggcgaag attggggaga gtaaatgtcg
     3841 aaactccgta tcgtatttga cagagtaagg tcagaggaga aaatgctcga ggaaaaagca
     3901 atcgagcttg gccacgatac aaagatgatt gatgcaaaga tgactcagat caatactgaa
     3961 agtaagaaat cagattttga ttttggtgat gtcgtgctgg agagatgcgt cagttatttt
     4021 cgtggtctcc actttactgc atgcctagag tttcttgatg ttccagtcat aaacaaattt
     4081 gaagtggcaa acaactgcgg aaacaaattg ataacatcgc ttttgctaaa aaaacacaac
     4141 gtgcccactc ctaggacata ctttacattt tcatcagaga cggcgcttga gaatcttgag
     4201 aaggtagggt atcccatggt catcaagcca gtagttggaa gctggggaag gggagtcatg
     4261 gctctaaaag acagggacac tgcagatgca ataattgagg taagagagct taacgacggt
     4321 ccgcttgaca ggatttatta tttacaagag atggtgaaaa ggcctccacg cgatatacga
     4381 gtcattgcgg taggagatca tgtagtagct gcaatgtatc gaacttcgac tggaggcttc
     4441 aaaacaaaca ttgcacttgg agcagaacct gtaccttgtg aaataacaaa agaccttgaa
     4501 gacatttgtc ttaaagcatc aaaggcagta ggtggaggaa tacttggtat tgatttaatg
     4561 gaagatgaaa agcgcggatt ggttgtacat gaagtaaaca acaccgtaga gttcaaaggc
     4621 ttggcaaaag ttgcaaaaaa gaatattcct aaagagatga ttgattttgc catcaagtca
     4681 gctataagat aaattatact atgttatgct agaggagtat caatgagagt aggagtaata
     4741 ggtgcatcag gttacgtcgg tggagaaatg ctaaggctac ttgtaagtca tcccaaagtc
     4801 gaaatcgcaa tggtcacatc aagacaatat gttggagaat atgtttcaag aatccagcca
     4861 agtctcaaga gttttatcga cataacattt tccgagcttg actatgacaa actttcagac
     4921 aagtgcgata ttgtttttac tgcagttcca catggaaccg caactgagat agtcaaggca
     4981 ctatatgacc gaggaatgaa ggtaatagat cttagtgcag actatcgatt acacaatcca
     5041 gcagactatg acaagtggta cggctggcag catccacatc cggaattgtt atcaaaatca
     5101 gtttttggag tgccagaact tcaccgagaa gagatcaaaa aagcgcagct tgtttcgtgt
     5161 cctggatgca tggcagtaac atcacttttg gctctttatc ctcttgtgaa aagaggtatg
     5221 attgacactg aacacatcat agtagattca aagattggct cgtctggagc aggagctgga
     5281 tcagttgctg gtacacacca tgcaatgagg tctggagtaa tcagaccata caaaccagca
     5341 aagcacagac atactggaga gatagagcaa gagctaagca tagctgctgg aaaaaagatc
     5401 aaggtatcca tgagtccaca tgcggttgat atcgtaagag gaatcttgtg tacaaatcac
     5461 actttcctta caagacctac aactgagatg gagctctgga agatgtatcg tgaacaatat
     5521 caaaacgaga gattcatcag attgatcaga gacaaaaaag gattgtacaa attcccagat
     5581 ccaaagtttg tagtaggatc taacttctgc gatgtaggat ttgacattga tgaagacaac
     5641 aacagactgg tcgtattatc agcttcagac aacctcatga aaggtgcagc aggctccgcc
     5701 atacaaaaca tgaacgtgat ggcaggctat gatgagatgg atggtcttca ctactcgcca
     5761 ctgacacctg tgtaaaaaat gatcacaatc aagattggag gaagtgttgt agatgggctt
     5821 catccatcaa caatttccga cctcaaaaaa gtatcacaga aagaaaaaat aattctagtt
     5881 catggcggcg gaaaagaagt gacaaagatt tcagaggcac ttggcaaaga acaaaaattc
     5941 attgtctctc ctggaggaat taaaagtaga tacaccgaca aggaaactgt agaaattttc
     6001 accatggtaa tgtcaggcaa aataaataag atgattgtag gcatgctcca aaaaaatgga
     6061 atcaatgcag ttggactttc aggtgtagac ggacagataa ttcaggcaga acgcaaaaag
     6121 aaacttgtgg tcattaatga aaagggtcgc aagatggtaa tcgacggagg atatacagga
     6181 aagataaagg atgtaaacac cagtttgata aagtcaattt tggatcaagg gtacgtccca
     6241 gtaatatcac cgattgcaat tagcgaagag tttgactttc ttaatgtgga tggagacaga
     6301 gctgcagcat atgttgcagg aaagatgcag tcagacaaaa ttttgttttt gacaaatgtt
     6361 gacgggctgt tgatgaacga caaacttgtc aagaacctgt ccctagcaga agcaaaagag
     6421 atgcttccaa agattggatt tggaatggaa aagaaggttc ttgcggcaac tgaggcattg
     6481 gagatgggcg taaagcaagc tctcatatca aatggtcaaa gagaaaaccc aatatcggta
     6541 gcaattgcaa acgataactg cacggtgatt acaaaatgac tgaagaccaa tttatgggaa
     6601 acatctacca gaggtttcct gttactgtag agcgaggact ggggtctcat gtatgggaca
     6661 ttaacaataa ggaatacatt gattgcatgg gaggatacgg tgttgctctt gttggtcaca
     6721 gaaatccacg cgttgtaaag gcacttcaag ctcagctcga aaaaataatt acagtacaca
     6781 gttcattata caacaagaca cgtgaagaat ttcttgaaag tctgatcaag attgcgccaa
     6841 agaatctttc acaagtttac ctaaacaaca gcggaacaga agctgtggaa gctgcaataa
     6901 agtttgcaag aaaattcacg ggcaaaagtg gaatgatagc aatgaatgga tcatatcatg
     6961 gaaaatcatt tggttctctg tcactgacat ttaatccaaa atatcgcaag tcatttgaac
     7021 cattagttga aaaagtttct ttttcaccgt ttggtgacat tgagtctcta aagtcatctg
     7081 taaactctga tactgcattt atcataatag aaccaataca gggagaaagc ggaatacatg
     7141 tagctccaga tggattctta caggatgtaa gaaagttgtg tgatgaaaaa gggatactgt
     7201 taatctttga tgaaatccaa gcagggcttg gcaggactgg aaagatgtgg gcaggccaac
     7261 actgggacac cactcctgat ataatgtgcc ttgcaaaagg aattgccggt ggagtgccca
     7321 tgggtgcaac acttgttcgt ccggacattc ttgcatgcat aagcaaggga gagcagtcat
     7381 caacatttgg cggaaatccc ctctcatgcg cagcaggaat tgctacaata caggcactaa
     7441 ctgaggacaa attggtagaa aatgcagaaa agatgggaaa gaaattccga gaaggattgg
     7501 aatcactaaa ggaaagacac aaagtaatca gagaggttcg aggcaagggt ttgatgattg
     7561 gcgtagaaat gaaattcgaa gtcaaagata ttttgtttga tggaatagca aacggtctgt
     7621 tgcttttgta ctctggaaaa aacatactca gattgcttcc tccgcttgtc acatcagagg
     7681 cagacataac caaagcttta gaaatactgg acgtgttgtt agaaaaagag gaaaagcgta
     7741 gaaatgtcta gagacaagac agatgaaacc ataattgaaa tgctaaagaa aaactctaga
     7801 gagtcttttg tagagatagg tcaaaagctt ggactgtcag aatctgcggt aagaagaaga
     7861 gtgaaaaatc ttactgatgg cggaatcata aaaaaattta caatagaaac tggtgatgca
     7921 aacgcaacaa gtgcaatcat tttgatctca gttgactctg caacagacac ttcaaaagtt
     7981 tctgcaaaac taacaaagct tcaaggagtt cacactgttt atgaaataac aggacagtat
     8041 gacattgcag taataatcag agcaccaaca attactgaca ttaacgtgtg cattgattct
     8101 ttgcgcaagg ttcctggtgt ttttgacacc aacacagtaa tcattttgcg cactgtggcc
     8161 taaaaacgaa attcgtatac ttttgccaag cagtgacgta tatcgttgca aaagtatcaa
     8221 aattagccga ttcctgtacg aatatgttta tattcgacag ttttttgtac cagatcagca
     8281 atggatgatc cgaattacta cgcataccag tacaatgcta tgggacaaaa accaaaaaag
     8341 attcatgtcc ttgatagcac cctacgggaa ggtgaacagc atcccggagt tacgttttcc
     8401 aacaagcaaa gaatccagat tgcatggatg cttgattatt tcggtgtaga tcaaatcgag
     8461 atttctccag tagtatcaaa cgatcacaag gaagcaacaa agacaatcat caagcaagga
     8521 ttaagagctg acattgttgc acatgtacgc gcgctcaaag aagatgttga tgttgcaatt
     8581 gattgtgatg cagtatggat ggcaacatat cttggaatgt cagacattca tctaaaagac
     8641 aagctaagaa tttcaagaga agaggcaatg cgacgagctg ttgaaactgt agagtatgca
     8701 aaatcgcatg gactaaagat gagattcacc atggaggatg caagcagggc agagccagag
     8761 tttcttatta aaatgtgcaa agcaatcgag gatgctggag tggacagaat aagcatccca
     8821 gatacagttg gaataatgag accaattgga atgtacaatc ttgtcaagac agtacgagat
     8881 cagataaaaa ttccacttga tgtgcactgc cataatgata ttgggtttgc attagcaaat
     8941 gcttttgcag gctgcgatgc tggtgcagat caaattcata caacaataga tgggattgga
     9001 gaaagaactg gaattcccgc acttgcagaa acggcagttg ctttgactta tcttttcaaa
     9061 tctccaaaca atttcagact ggatatgctc atggacctct ccagactcat tgaacaatat
     9121 acaacaatca agccatatga ctccaagcca atagttggct ctacagcata caagcacaag
     9181 gcaggaacac acctagcagc aattctgaga aaccctgcag catacgagcc aatcgagccc
     9241 aaagtggtag gaaacagacg caggatagta tttggcgaac ttgcaggcaa aaacggcgca
     9301 gcatacctca tgtcactttt gggtctagac caaaacgacg agcatgcaaa agcggtagcg
     9361 gcaggactaa agaacctgag gatgggcgac ttgcttgaga ttccactaga tgacaggctg
     9421 gaaagaaaga taattaatga cacccaaatt aaagtaaaac caggtaagta agatgaaatg
     9481 tttagaatgt gatgcgacaa tttctcctcc aggagatgca atagaaggcg agattgtaac
     9541 ttgtcctgat tgcggcgcaa gctttgagtt agcaaaagga tcaaccggct ttgagttaaa
     9601 accagcacaa actgttggtg aggactgggg ccagtgagct ccggattatc gattctttac
     9661 gacaccatcc ggtgggaaga aaaagctctc cttgacgcag gcaaaaagaa aggaatcgac
     9721 atcgagatgg tcgattgtaa gaatctgttt ttgaatttgg gcaagaccaa agaaaaattt
     9781 gataccgtaa tacagcgatg tgtaagctat taccgtagtc ttcactctac tgcagcactg
     9841 gaaggaaaag gaatcaatgt gataaattcc ttaaacacaa gtatctttgc aggaaacaag
     9901 ctcttcactc acatgctttt gcaaaaattt ggagtgccaa ctccatttag tacagtggca
     9961 ttttctgaag aagctgcatt ggaggctcta aaagaaaatg gatatccaat ggtactaaag
    10021 ccaacagttg ggagctgggg gagaatgatc gcgcttctaa aagatcagga ttctgcagaa
    10081 ggaataatgg aaagcagaga aaggatgtac ccaatttacc aagtgtacta tctagaagag
    10141 tttgtccaaa gaccgccacg agacatcagg gcgataatgg taggagacaa ggtagtggca
    10201 gcaatttacc gatattcagg agatggacag tggaaaacaa acatggcact tggaggcaag
    10261 gccgaagaat gtaaggtaac aaaggaacta gaagacattt gcgtcaaggc aaaaaatgca
    10321 gtacagggac agatagttgg ggtagatctg atggaaagca aggaaaaagg gctggttgta
    10381 cacgaagtaa acaacacaac agaatacaaa aatactgtca gagttactgg tgtagatatt
    10441 ccagcattga tgatagatta cgcactacaa tcgagaaaat aagaattgga ctcttcaatt
    10501 accataacac caaggttttc agtaaagatg cttgagaaag cacttaggct ttacactcca
    10561 tcgcttgcag aaaaaccatt agcagaattt ttggcagaca agtgcgatga cttgggattt
    10621 gacaacatcc acatagatga agttggaaac ctgattgcaa caaaaggatc aggctcgcct
    10681 aagatacttt tgtgcggcca tatggatact gtcccaggca agatcaaagt caaaaatgat
    10741 aatgggtttt tgtacggacg cggtgcgtca gatgccaaag ctcctctaat ggcaatgctt
    10801 ctttctgcgg cttcgatgca aagtaacagt ggaactgtag tatttgctgc agtagtagat
    10861 gaagaaggaa atgcaacagg aatcaaaagc ttagtaaaac aaaagcttga cattgattac
    10921 gcaatttttg gagaaccaag tggaatacga aacatcacaa tagcatacaa gggaagaatt
    10981 gcaataaatc tcaaagtcaa tgtaggaaac agtgcacatg caagtgcacc atggcttgca
    11041 aaaaatgcaa ttgaagaagc atacgtgttt atcaatgcac tgaaaaagtc tttggaaggc
    11101 ggtcaggaaa acaagccaaa aggaatgttg cttacagcaa cgcttactga gataaagggt
    11161 ggaacaagcc acaacgtaac agctatagaa tgtgatgcga taatggacat taggattcca
    11221 gtagac
//
LOCUS       DATIDL010000081         5255 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrosopumilaceae archaeon isolate SMAG_U16342
            k141_1309391, whole genome shotgun sequence.
ACCESSION   DATIDL010000081 DATIDL010000000
VERSION     DATIDL010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935836
            Sequence Read Archive: SRR25158326
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosopumilaceae archaeon (soil metagenome)
  ORGANISM  Nitrosopumilaceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrosopumilales;
            Nitrosopumilaceae.
REFERENCE   1  (bases 1 to 5255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/04/2024 05:09:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,520
            CDSs (total)                 :: 1,474
            Genes (coding)               :: 1,474
            CDSs (with protein)          :: 1,474
            Genes (RNA)                  :: 46
            rRNAs                        :: 1 (5S)
            complete rRNAs               :: 1 (5S)
            tRNAs                        :: 43
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5255
                     /organism="Nitrosopumilaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1309391"
                     /isolate="SMAG_U16342"
                     /isolation_source="soil"
                     /db_xref="taxon:2202732"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..269
                     /locus_tag="VLB45_03505"
     CDS             <1..269
                     /locus_tag="VLB45_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010191831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HSD04802.1"
                     /translation="QYADPKEVEEFTLGINPHEYKSNQLSYLLTFIATDIMTPNPVVI
                     DAKEDVSTAALLMIRHGISGLPVVRNSLLVGILTKTDIAKAIAS"
     gene            complement(290..718)
                     /locus_tag="VLB45_03510"
     CDS             complement(290..718)
                     /locus_tag="VLB45_03510"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HSD04803.1"
                     /translation="MSKKVISIESTVSATEIAKLMDRNKVSCIVLTKNEKPYGMVTER
                     DLLTKITALNKKSSELNANEIMTSPITTVSSMTPIEEVAEKMILNKMRRVIVSDNEQP
                     VGIITVTDFAKHINTIISGSENYNKEVYDNLFDEYEYLNG"
     gene            1100..1372
                     /locus_tag="VLB45_03515"
     CDS             1100..1372
                     /locus_tag="VLB45_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04804.1"
                     /translation="MGIPPEVKQIVEKQMEVMIEQTKAYLPFIKLAFPGVKDLSEPCY
                     ALIVGNVLPIFVTQYAMRMKYPQEQDFVEFGEIAQKYKEKVKLMFQ"
     gene            1369..1818
                     /locus_tag="VLB45_03520"
     CDS             1369..1818
                     /locus_tag="VLB45_03520"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HSD04805.1"
                     /translation="MIIKNILIPYEGSQLSKKALDLGKEIARNFNANLELLMVIPLFY
                     PMSEPSIYSGATIGDYGKIIKELKTRGEKEIKKVTEKCREEGVKAFYKVTEGDVSYTI
                     LKYAKKNKTNLIIMGSRRMKGLGAIKRLGSTARFVSEHATCPVTIVH"
     gene            complement(1833..2831)
                     /locus_tag="VLB45_03525"
     CDS             complement(1833..2831)
                     /locus_tag="VLB45_03525"
                     /inference="COORDINATES: protein motif:HMM:NF013895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HSD04806.1"
                     /translation="MSTKSNSKNTSGIAGLKSTSKFVAVTKNDTVDKVKKIIMDKTRE
                     LEVIDEVYIVNENNVLEGAVSLKEILQSPDETKIESIMKKNILSVRYHSHQERVIQVA
                     LKNDLKAVPVVDKENHLLGVIPHDIILDIFHHEFREDILSSGGIRHKDKEIEKIATPV
                     SRLIKARMPSLIIGLSGGLIAAYIVSGFEEVLRSYLVLAAFMPVMIYLSDAIGTQSQT
                     LIVRMIAMEPKFSLKRYLTREIKIGASLGAIFASLLFIATIFGFGSHIHLAIIIGTSM
                     FVSMLIQTFVATYLSVMLAKSHVDPAIASGPIATIISDITTVIVYFGIATILLENL"
     gene            complement(2834..3217)
                     /locus_tag="VLB45_03530"
     CDS             complement(2834..3217)
                     /locus_tag="VLB45_03530"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HSD04807.1"
                     /translation="MTTSALFEKFWMSLIDSTKDDYINKELIPLSYFIEKDSKWILEI
                     DLPMVNKNDIAITLTEEHIVVTAKLEKTHCISKNNCMTEFNYFKKVVSLPTGIDKEKI
                     SAKFNGGILNIQMPKIDTGKKIRIE"
     gene            complement(3224..3949)
                     /locus_tag="VLB45_03535"
     CDS             complement(3224..3949)
                     /locus_tag="VLB45_03535"
                     /inference="COORDINATES: protein motif:HMM:NF021287.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAC2 family protein"
                     /protein_id="HSD04808.1"
                     /translation="MNIDKPRKSLSPVLIIGIPGPGLIGTLSLSYIIHSLGMEVIGEI
                     EHPDISNVIFVDNGNLAGPIRVYRKGLVYAVLSDIPIDYVIAAGFTESVIDFCKKNKI
                     DKIIVLSGLQATERSVDKLKTYGLVTNERLDKILYENEIPKFLTGVIAGPDATILTKL
                     KNSPIPTLVLYTECNFFFPDPEAAIHTINIISRILKMEIDTTKFKKQVDFLRLQSRQL
                     MEDTMAILNPEKAPPILAPQIYR"
     gene            complement(4042..5211)
                     /locus_tag="VLB45_03540"
     CDS             complement(4042..5211)
                     /locus_tag="VLB45_03540"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HSD04809.1"
                     /translation="MNSDQSTYEYVKEVQKMPIRKTLHPATIVEPSFSISKVIDMMSK
                     TDSYDVYCMNEGTVLTTNAREILTVRDISTMKISSLLHKIRTLSKDDTIEKAATAMAH
                     YRMRSFPVTDNGHLIGVVTAKNVVNLLSRQNLKWIRANSVLTPNPLTLSSAESLAKAR
                     KIMTTNRIDHIPILHKGKISHVLTSMHLLQTLTPPERIGSDQKGINMHRRLESDIGNV
                     GSPRIPQCNTNDSISTIMDTMLKTNTTCCLLTLWDNLHGIITYKDILNLLVTKIKSEI
                     PLYIVGMPSDMSNAEIVKTKFQKIIKNLIKVYPEVEEARTSIKSIHSPLSKRQHFEVS
                     IRISTPYKTYNYKELGWDLSQVFDALGKSIVRNLSKRSKRRWKTSIRKIDKKNIF"
BASE COUNT         1619 a          898 c          884 g         1854 t
ORIGIN      
        1 aacaatatgc tgatccaaaa gaagttgaag aatttacttt gggaataaat cctcatgaat
       61 acaaatcaaa tcagctaagc taccttctta cgtttattgc caccgacata atgactccaa
      121 atccggttgt gattgatgca aaagaagatg tgtctactgc cgcattactg atgattcgtc
      181 atgggataag tggattgccc gttgtaagga attcattact ggtaggcata ttgacaaaaa
      241 ctgacatagc aaaagctatt gcatcataat ttattaatta ttaagagttt tagccattca
      301 aatattcata ttcgtcaaat aaattatcat aaacttcttt attgtaattt tcagaacctg
      361 aaataattgt attaatatgc tttgcaaaat ctgtgacagt aattatccca actggctgtt
      421 cattatctga tactatcact cgcctcattt tatttaaaat catcttctcg gcaacttctt
      481 ctattggcgt catggaggaa accgttgtaa tgggagatgt cattatctca tttgcgttga
      541 gttcggatga ttttttattt aatgctgtaa ttttggttaa aagatctctt tcagttacca
      601 taccatatgg tttttcattt ttcgttaaaa ctatacaact tactttgttt ctgtccataa
      661 gcttagctat ttcggttgcc gaaacagttg actcgataga aataactttc ttgctcatta
      721 tatcgcgtgc aacaatcata tgaaaaaact tctttatttg cctataaaag caaacaaggt
      781 aattctcgaa tcaaattatc acatttgata ataagctaaa atcatagatg ataatctgca
      841 ctagttcttt aatattctaa aaatgattct ggtttaggtg caaaatttgg gagatcgatt
      901 tctctataat gatttgtata atctgaaaaa gaaaagtctc cttaaatcca ctgtgatacc
      961 gcttacctgt cacgtatgta agaaagaatt gaacggaatt tctattgctg caaaaataat
     1021 agaaggtaaa accgtttttc tttgtacacg tcattatcaa gctaaacctt atcaaatgat
     1081 agttgccgag taaatcaata tgggaatacc acctgaagta aaacaaattg tagaaaaaca
     1141 gatggaagta atgattgaac agacaaaagc atatttgcca tttataaaat tggcatttcc
     1201 tggtgtaaaa gatctgtctg aaccttgtta tgcattaatt gttggaaacg tgttacctat
     1261 ctttgttacc caatacgcta tgagaatgaa atatccacaa gagcaggatt ttgttgaatt
     1321 tggtgaaatt gcacaaaaat ataaagaaaa agtaaaactg atgttccaat gataataaaa
     1381 aacatcctta tcccatatga ggggtctcaa ctatcaaaaa aagcacttga tctaggtaaa
     1441 gaaattgccc gtaatttcaa tgcaaatctt gaactactaa tggtaattcc actgttttat
     1501 cctatgagtg aaccttcaat ctacagtgga gctaccatag gagattatgg gaaaattatc
     1561 aaagaactaa aaacaagagg tgaaaaggaa atcaaaaaag ttacagaaaa atgtagagag
     1621 gaaggagtga aagcatttta caaagtaacc gaaggcgatg tatcatatac aatcctaaaa
     1681 tatgcaaaga aaaacaaaac gaatctcatt atcatgggaa gcagacgtat gaaaggttta
     1741 ggtgcaatta aacgactagg aagcactgct agatttgttt ctgaacatgc aacctgtccg
     1801 gtaactattg ttcattaatt cttggcattg aattaaagat tctctaaaag tatagttgct
     1861 atgccgaagt atactatgac cgttgtaatg tcgctgataa ttgttgcaat aggtcctgat
     1921 gcaatagcag gatccacatg agattttgct aacattactg atagatatgt tgcaacaaat
     1981 gtctgaatca acatacttac aaacatagaa gtgcctataa ttattgccaa atgaatatga
     2041 ctcccaaacc caaagattgt tgcaatgaaa agtaatgatg caaaaatggc gccaagactt
     2101 gcaccaatct tgatttcacg tgttagataa cgttttaaag aaaacttggg ttccattgca
     2161 atcatcctaa caataagagt ttgggattgt gttcctattg cgtcacttaa gtaaatcata
     2221 actggcataa atgcggccaa gaccagatat gatctcaaaa cttcttcaaa accgcttaca
     2281 atgtaggcag ctatcagacc acctgacaat cctatgatta atgacggcat ccttgctttt
     2341 attagcctag atacgggcgt agcaatcttt tctatttctt tgtctttgtg acgaatgcca
     2401 ccagaactga gaatatcttc acggaattca tgatgaaaaa tgtctaaaat aatatcatgt
     2461 ggtatgacac ctaaaaggtg gttttcttta tctactacag gtactgcttt caagtcattt
     2521 tttaacgcaa cctgaattac tctttcttga tggctgtggt atcttactga caatatgttt
     2581 tttttcatga ttgattctat ttttgtttca tccggagact gtaaaatctc ttttaatgaa
     2641 accgctcctt caagtacgtt gttttcattt acgatatata cttcatctat gacttctaat
     2701 tctcttgtct tatccattat gattttctta actttatcaa ccgtatcatt ttttgtaact
     2761 gcaacaaatt tactagtact ttttaaacct gctattccag aagtattttt ggaattagat
     2821 tttgtagaca aaattactca attcgtattt tttttccagt gtcaatcttt ggcatctgta
     2881 tgttaagtat gccaccattg aactttgcag aaattttttc tttatctatt ccagtaggaa
     2941 gtgacacaac ttttttgaaa taattaaact ctgtcatgca gttattcttt gaaatacaat
     3001 gtgttttttc taattttgct gtaaccacaa tatgttcttc tgtaagggtt atcgctatat
     3061 cgtttttgtt taccattggt aaatcaattt ctagtatcca tttggaatct ttttctataa
     3121 aataagataa aggtatcaat tccttgttga tataatcatc ctttgtacta tcaatgagtg
     3181 acatccaaaa tttttcaaag agagcagatg tggtcatgac tttttacctg tagatctgcg
     3241 gcgccagtat tggaggtgct ttttctggat ttaatattgc catggtatct tccataagtt
     3301 gacgactttg tagtcgtaaa aagtcaacct gttttttaaa tttggtagtg tcaatttcca
     3361 tcttcaatat gcgtgagatt atgtttatgg tgtgaattgc tgcttctgga tctgggaaaa
     3421 agaaattgca ttctgtatac agtaccaagg tgggaattgg tgaatttttt aatttagtca
     3481 atattgtggc atcaggaccc gcaattacac cagtaagaaa ttttggtatc tcgttttcat
     3541 ataggatctt gtctagtctc tcgttagtca caagaccgta tgtctttaac ttgtcaacac
     3601 ttctttctgt agcctgcaaa ccactcaaaa ctatgatttt atcgatttta ttttttttac
     3661 aaaaatcaat tactgattct gtaaacccag ctgctatgac ataatctatt ggaatatctg
     3721 aaagcactgc atacactaaa ccttttctat atactctgat aggtccggca agatttccgt
     3781 tgtctacaaa aatgacattt gaaatatcag gatgttcaat ttcaccaatt acttccatgc
     3841 ctagtgagtg tatgatgtag gaaagtgaaa gagtgccaat caatccaggt cctggaatcc
     3901 ctatgattaa tactggagac aacgattttc ttggtttatc gatattcata ctatgacgtc
     3961 tggttatcct catacaaaaa acaacgtaat attttcacca ttgataatca tgcttgacat
     4021 tctgtctgga tgagaatatg attaaaaaat attcttttta tctatttttc ttatcgatgt
     4081 cttccaacgt cttttactac gcttagaaag atttctaact atgctctttc caagtgcgtc
     4141 aaatacctgg gaaagatccc atcctaattc tttataatta taagtcttat agggtgttga
     4201 tattctgatc gagacctcaa agtgttgtct cttgctcaac ggactgtgta ttgattttat
     4261 tgatgttctt gcctcttcga cttcaggata tactttaatg agatttttga ttattttctg
     4321 aaattttgtt ttgacaattt ctgcattaga catgtcagaa ggcatgccta ctatgtagag
     4381 tggaatctcg cttttgatct ttgtcacaag aagattgagt atgtctttgt aggttatgat
     4441 tccatggaga ttatcccata gtgttaacaa acaacacgta gtattagttt tcaacattgt
     4501 atccattatg gtacttatcg aatcattggt gttacattga ggaatgcgtg gacttccaac
     4561 attgcctata tctgactcca accttctatg catgttgatt cctttctgat cacttcctat
     4621 cctttctggt ggcgtcaatg tctgtagcaa gtgcattgat gttaagacat gactgatctt
     4681 gcctttgtgt agtattggaa tatgatctat tctatttgta gtcatgattt ttctggcttt
     4741 ggcaagggat tctgcactac ttaatgttag tggatttggt gtcaaaaccg agtttgccct
     4801 aatccatttt agattctgtc ttgataacaa atttaccacg ttttttgctg ttactacccc
     4861 tattaggtga ccattatctg tgacaggaaa agaacgcatt ctataatgcg ccattgctgt
     4921 agcagctttt tctatcgtat catctttaga taatgttcga atcttgtgta acaaagagga
     4981 gattttcata gtggaaatat ctctaactgt taggatttca cgtgcattag ttgttagtac
     5041 tgtgccttcg ttcatacaat atacatcata cgagtcagtt ttagacatca tatcgataac
     5101 tttggaaatt gaaaacgatg gttcaactat ggtagcgggg tgtaatgtct ttcttattgg
     5161 cattttttgc acttctttta catattcgta tgtactttga tctgaattca ttagtatacc
     5221 tccattgcaa ctcttttaat tggtatataa ttcat
//
LOCUS       DATIDL010000082        16906 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrosopumilaceae archaeon isolate SMAG_U16342
            k141_2928993, whole genome shotgun sequence.
ACCESSION   DATIDL010000082 DATIDL010000000
VERSION     DATIDL010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935836
            Sequence Read Archive: SRR25158326
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosopumilaceae archaeon (soil metagenome)
  ORGANISM  Nitrosopumilaceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrosopumilales;
            Nitrosopumilaceae.
REFERENCE   1  (bases 1 to 16906)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16906)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/04/2024 05:09:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,520
            CDSs (total)                 :: 1,474
            Genes (coding)               :: 1,474
            CDSs (with protein)          :: 1,474
            Genes (RNA)                  :: 46
            rRNAs                        :: 1 (5S)
            complete rRNAs               :: 1 (5S)
            tRNAs                        :: 43
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16906
                     /organism="Nitrosopumilaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2928993"
                     /isolate="SMAG_U16342"
                     /isolation_source="soil"
                     /db_xref="taxon:2202732"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            85..4263
                     /locus_tag="VLB45_03545"
     CDS             85..4263
                     /locus_tag="VLB45_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007403038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HSD04810.1"
                     /translation="MFAKQSYGFYDVDLPELNTTFDLKENSNIMTISPTIKENDIKRY
                     VVFGSGTISDVRSIAQNIVYQINSDRGFFSVGIFSQTDIQDLKSKGYTVIEDFPLEFD
                     STPIDSVREASRIGTITGSDEVSQKYGFTGKGVTIGIVDTGTDFSNPDIQHSLARDEN
                     NHPIMIDADGQGLVLTNATFIANINKKNVIVNYSKPVPENITSSVYVNSKGVFLDLAK
                     KGNGTSVLVFNSLFPSGGTPVLNGTISNDMKIGKSKTDYIVSKSGIYHLGVIYQSVIQ
                     GPFPRLQLVPVLVIDSKVAGLYDSVIPDMSTSWTDYTRFDSNNAKPVVYDFDFTDEIP
                     MTLGNGKEFFLYDYDKDGKFDYSAGTFGARVLDVYGVITNSSNIDKKIRATNGTLLLP
                     LDPDGNYFGVMYDFVGHGTSSAASITSKGTEKYDIYKNSTKYTIKGVAPSAKIVPIKA
                     LWFGDAVYAWLWAAGFDQKNNNWNFEGKPRVDIISNSWGISNFPSIQSVSGLDILSLV
                     LSVLVVPSSIDEDYPGVLVVSSAGNAGHGYGTLGLPNASPYGLSVGATTNNVYVGYGP
                     FKNQPRFGNSTFSSNEIVDFSSRGPGIIGDPKPDLMSIGAYGFTPTSVTKTSKNATDP
                     FAIFGGTSMAAPLVSGSAAVLIESLKQKGEQYDPFRIKNILMSTAADLENDPFSQGAG
                     LVDLENAVNFVQGKSGTFIVHNDATYHNVKKILDVPINAINSSSFGIERFQLSNATFP
                     ETAWFGGRLYPGERTFTTFTIENPTNRTLDITITPQTLQLIKKYQYQGKTEVNLKDPL
                     LNKSGTFRPNYLHLKDVAKDTGLLFEKAKPIPNDSSLMVLNVNFPFDQFMNSTDNMYA
                     NDMKISSLYLYDWNDKNNDGNTTYNELTLVNRGGSWGTVQEMRVSEPNTKFDNIPLVG
                     VYPVPTRYSYWIGDTKKNSTSMDYTVTTSFYKKENWDAVWVNYKQIQIPPNSSTELVA
                     TLVAPLDSKPGVYQGFMTFKGDSHEVNAPVSFAVKKKVQAKDMPTVIEGSTTDDVLYG
                     NGYISGAFDMTNRYNAGDWRQYYFDVEDETINSLALDISWENKNTNLSVFVIDPVGRI
                     VQTNVPPGVLGHFQGWPTGDWLGTTPFSEGGGFFPIKNKDDTSTVLFAPINQTGTYSL
                     LMHSTLFGGQSVVEPITVTAKFSTILPDEKDPQILSTIPEFLNSTHIVVPEIIEENLD
                     ALTYYLDENKIQGIDKKERSVPSKLLTEGTHTLRVVAKDTVGHYAEKTLTFTVDDTPP
                     IILIKSPQNGSTVSNVLTIDIDVSELHMPDSEGITVILPNGTISDKTFVQFDTRTIEN
                     GKYDIKVSAKDMAGNEISKKITVNVDNNQLFENPITSPDQSFILLEGILIGIAIATIA
                     LLITLKKIKISKKN"
     gene            4304..4915
                     /locus_tag="VLB45_03550"
     CDS             4304..4915
                     /locus_tag="VLB45_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010194930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HSD04811.1"
                     /translation="MSEQDSSAKGTLSRRDFLKLMGVAGTALTFTPFIPWGKFMPNPD
                     TASLDKAKVILPDDTQANVNTFPVNHAEVITYPKTGDRVLDEEAFRKWQFIRLPSEVG
                     GDAKDVTAFRAYSMVCLHLWCLWKYWPEERRRRGECPCHGSMYNVLTGQAFAGPASLQ
                     APPSNTLARLTLEADNEGFLYISPAKWSVNENGVVGYGRFIKA"
     gene            4893..6455
                     /locus_tag="VLB45_03555"
     CDS             4893..6455
                     /locus_tag="VLB45_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HSD04812.1"
                     /translation="MVVSLKRRNGLVDFLYWVWDGIDRTVFIGTKFSFPARFVSPFGF
                     LGMLTFVTFIILGITGALLMFYYEPILDRAWDSVEKINNNVPFGFHLRNIHYHASNAM
                     VLLAILHMYYQYFSGRYKIRNEILWVTGVILGTVTILEAFTGYDIIFSERAELAISIA
                     ASLTNSIPIAGPTIRDAMFGSGFADFVLRFYAQHVFVLPIVMLGLMAVHMPRFLVFDV
                     PMVMAISGAILITGGVFPVDLGFKFEPTVPPGITVPEWYLTGLYAFLRTQYDKFVTGV
                     AWPGLFILALLITPFIDRYKKFSWKDRPMVTAFGITGIAQIMVTTYWGFYISPDTTMP
                     LVERLVIDPIFFYLVMILLVPLGFGFSYMMIKMSKEAERKAKLAASKGPQKVAQINLS
                     AKWINWLIVALLGFQVYLNIAAYNAALSGMNNFSLFLTGLILLVFAGLFHVYRYGMNE
                     AKKIPTPPPAIAKEIPKPIGVEPTTKLPEKDKTSDSKPSAPKPTAPEIKTISTVDTTN
                     KINADPSEIKKL"
     gene            6513..6989
                     /locus_tag="VLB45_03560"
     CDS             6513..6989
                     /locus_tag="VLB45_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HSD04813.1"
                     /translation="MSTPTSSHAYGIGIIAMVIGLAVGISYYQLYYIPELTAKPKISE
                     EVLNPVEAENLINMIKGSSSPEQQDNFVPKLVNIQLGIDNHVVWENIDETPHTVTADA
                     GHEYTDKYSGKFGSLGVVKPGERYEFLFTEPEVEIEYHCEPHPWMKGKLVITKARF"
     gene            complement(6986..7336)
                     /locus_tag="VLB45_03565"
     CDS             complement(6986..7336)
                     /locus_tag="VLB45_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007551391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04814.1"
                     /translation="MKDVELALENKNIIPFTEIKGKIQVRYPGRYDGVVINTQISNSN
                     QLIVYKSYNDKPISQNLSRLFISKDHMPDNKAEFTAAIEFKPTESHDVKFRVSIIQEH
                     KEIESDVLFAKLVV"
     gene            complement(7382..7456)
                     /locus_tag="VLB45_03570"
     tRNA            complement(7382..7456)
                     /locus_tag="VLB45_03570"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7419..7421),aa:Arg,seq:tct)
     gene            complement(7493..8341)
                     /locus_tag="VLB45_03575"
     CDS             complement(7493..8341)
                     /locus_tag="VLB45_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007551390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA glycosylase"
                     /protein_id="HSD04815.1"
                     /translation="MQEITKIDLDNTINSGQVFLWENIDGIWYGINGKEVFMITQNPF
                     KIISSQKKPTDLFRDNDNMKKILSDISKDSLVRSTTFQFPGLRLMRQDPFQCYISFIC
                     SSNASIPNIKQMLEKICKKFGKKINFCGRKFFTFPEPEKLANASLRDLLSCGLGFRAK
                     YVKSASRAVVSGKIDFDVLKKSDYKTAKEKLEEVYGIGDKIADCILLFSLEKIEAFPI
                     DRWTLRILQNYYANKFQKIIGKSLTKKKYMNLHEKIVEYFGPYAGYSQQFLFKMEREL
                     NNKKWL"
     gene            complement(8343..9599)
                     /locus_tag="VLB45_03580"
     CDS             complement(8343..9599)
                     /locus_tag="VLB45_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase RNA specificity
                     domain-containing protein"
                     /protein_id="HSD04816.1"
                     /translation="MQVKVLGAAQEVGRSAFQVNCDGANFLLDYGVFLGKEQQYPLHV
                     KPRDINAVVITHAHLDHSGYVPFLFVSGGCDVYATPPTLDLSRLLIEDMIRLGKNFLP
                     FEMKEVSNMMMRSKEIGYREKIKRGNASFELLESGHVIGGGAVVVESGNKKIYYTGDI
                     NPRGSRMLREADLDFGEVDLLITESTYSQTEQTPRNETEEKFIEFAYEVLDRKGSLFV
                     PSFSVERSQEIACVLKDAKFKHKVIMDGMAVKVNEIMLRYPEYLRDPKVFADAIHQAL
                     WIKGESERKKALNEPCVVISPAGMLVGGSAVYYLQQLALDKKNGIALVSYQGEGTPGR
                     KLLETGRVNIRGRDVKAESEVRQFEFSGHADRNGLFEIIKKIKGNPKILTVHGDKDSC
                     VKFAEEIHEKFGFDAMAPSAGETITV"
     gene            9713..9934
                     /locus_tag="VLB45_03585"
     CDS             9713..9934
                     /locus_tag="VLB45_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04817.1"
                     /translation="MGRTFEQWWNTIPKDLRDKVRKGDEGNKPLLNQINWVWVHNMMN
                     QKGDLNPSSAELLDWVTSGQIDAIRQSKK"
     gene            10059..10367
                     /locus_tag="VLB45_03590"
     CDS             10059..10367
                     /locus_tag="VLB45_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007403030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HSD04818.1"
                     /translation="MPIAILPDIDEQRCIGCALCVEICTSLGPDVLRVKPVEGWKRGK
                     AFVFYPERCISDGACIGVCPTKSIFWMRPMTYTAGQPVPLHKNGVFIKGWAEDAALSK
                     "
     gene            complement(10401..11918)
                     /gene="tgtA"
                     /locus_tag="VLB45_03595"
     CDS             complement(10401..11918)
                     /gene="tgtA"
                     /locus_tag="VLB45_03595"
                     /EC_number="2.4.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016939489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(15) transglycosylase TgtA"
                     /protein_id="HSD04819.1"
                     /translation="MFEIKKSDLAGRIGILRTNHGEVETPAYVPVVHPVKQAIPAKKM
                     RDMGFDLVITNAYITLNNYGQEAIKRGIHSIIDYDGAVMTDSGGYQVLEYGKVDVEAK
                     DMANFEQEIKTDIAIPLDRPTGYGLSKKKARSYVEHTLKVSKETLEQSEKNGQIWAGP
                     IQGGEHFDLVKRSTKTLVEYGFEMLALGSPVEFMESYEYKLLAKMIITAKKQMPDSIP
                     LHLFGAGHPMTIPLAVALGCDTFDSASYMLYARHDRYITEDGTVRLDEIAYFSCTCEV
                     CVKYTPKEMRALENNERANKIALHNLFAIKAEVDRVKQAIHDGRLWEYVIKKAHSHPK
                     LFETISTFVENSDYFIDRTPRFKEKAVFLFSKEDQFRPEVAAFHRMARKFKTKKDTLV
                     VIPDGTMRPFYLSGEYSKLRKKFIKKSQDVQFCQFNPFLGIIPLEISDMYPASHYVMP
                     RMQYDYTEFPEFTKTWKTFFSNNNFKTIYLAKDEFIKNHAKILPKKIKKIPISRF"
     gene            complement(11952..12506)
                     /locus_tag="VLB45_03600"
     CDS             complement(11952..12506)
                     /locus_tag="VLB45_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010194953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HSD04820.1"
                     /translation="MSKVITGSPGVGKHTVAKQLAKNLNLEIIDINKVAIEEGIFEKK
                     NETLDVDVKILKKIITKKNTKNSLIVGHLAPYVVSRKQVDVAVVLRKNPYSLSSIYKK
                     RKYSYEKSIENLGSEILGVTLYDAINEFGPDKTFQIDTTLKSVSTVVKKIADLFTKHI
                     FQEDEVDWLSLISEKNDLRRFFPY"
     gene            12580..13773
                     /locus_tag="VLB45_03605"
     CDS             12580..13773
                     /locus_tag="VLB45_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HSD04821.1"
                     /translation="MQIGLLGKTNVGKSTFFSAVTETTAQIGNYPFTTIEPNVGISYV
                     KSECACKHFGINHKHPLCINGTRFIPIKLIDVAGLVPGAHEGKGLGNKFLDDARQAEV
                     LIHVVDISGSTDIQGHSVPVGTHDPMEDVKFVEEEFDQWMKQILQREWQKLTREMESK
                     GAKLVQAIAQRFSGLGIKEYDVEIVIQHLNLQTRKPADWKDSDLFAFVKELRKNTRPV
                     LIAANKADLCHDLSIFEKLRKDRLAIACSAETELLLKKASKGGLIDYLPGDKTFKIKD
                     GVSLNPQQQKALDLVKMVFSKIETTGVQQALNAACFELLKLIVVYPVEDENKLSNKDG
                     EVLPDARLLPPSSTAKDLAATIHADLAKGFLHAVDVKTKQRIGAQHQLKNGDVIKIVS
                     TLSRG"
     gene            13775..14758
                     /gene="kae1"
                     /locus_tag="VLB45_03610"
     CDS             13775..14758
                     /gene="kae1"
                     /locus_tag="VLB45_03610"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061711 - N(6)-L-threonylcarbamoyladenine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KEOPS complex N(6)-L-threonylcarbamoyladenine
                     synthase Kae1"
                     /protein_id="HSD04822.1"
                     /translation="MLGLGIESTAHTFSCAILEKTGKHGKILSDVRKIYRPPDGEGIH
                     PREASRHHAENSPIVLSECLQNADVKITDLDIISYAAGPGLGPCLRIGAVVARSLASY
                     YNIPVYPVNHAIGHIELGKMLTGAKDPLVLLVSGGHTMILAFLSKKWRVFGETLDITL
                     GQLIDQLGRSAGFASPCGNKIEELALKSNNYLPLPYVVKGNDVSFSGLLSATKHVLSK
                     SLEDACFSLQETAFAMIGEAVERALSFTRKKELLIVGGVAANRRLSEILTSVCKRQGC
                     KFFVSPKQYAGDCGSQISWTGLLESSYKSGVSLDETFVKQSWRLDTVEVPY"
     gene            complement(14755..15078)
                     /locus_tag="VLB45_03615"
     CDS             complement(14755..15078)
                     /locus_tag="VLB45_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04823.1"
                     /translation="MEDSEKKIIQEDCSEDSMGSGILTLTNMRVAFDKTKGRIADFTK
                     KIGDTVIDVPLNQIISVGREGWLIKKIWLKAKTPDGEKTYKFGVYNTKKWLETLQETI
                     AQNKN"
     gene            15117..15737
                     /locus_tag="VLB45_03620"
     CDS             15117..15737
                     /locus_tag="VLB45_03620"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KEOPS complex kinase/ATPase Bud32"
                     /protein_id="HSD04824.1"
                     /translation="MKLLKKGAEADIYLTLWNGKKSILKIREKKDYRNSILDKRIREH
                     RTIRESSIISDVKSFGIPSPIVYCVDTKACRIYMQHISGNLVRDLPKSSVIKTCRDIG
                     KIVGTLHNNGIMHGDLTTSNFIVLNDKVFVIDFGLAQRTEKIEDHAIDLRLFKEILNS
                     AHADIMEKSWAGFLEGYKKIVGESRQNRVLSQVSIIEGRGRYANVV"
     gene            15730..16290
                     /gene="rdgB"
                     /locus_tag="VLB45_03625"
     CDS             15730..16290
                     /gene="rdgB"
                     /locus_tag="VLB45_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007551379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HSD04825.1"
                     /translation="MSSILFASSNKNKYEEAKDVLSKFGVDLKFFKCNLEEIQADTLE
                     EIALHKAQNAFLQCSRPVIVEDDGLFINSLNGFPGPYSSFAFKTLGNAGILKLLKSQR
                     KATFQSVIAYCESTEDVLFFKAVVNGKIARKLMGKRWGYDPIFIPEGQTKTYAQLENK
                     NFVSHRYLALKKFANWYLHERKSNDR"
     gene            complement(16250..16870)
                     /locus_tag="VLB45_03630"
     CDS             complement(16250..16870)
                     /locus_tag="VLB45_03630"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HSD04826.1"
                     /translation="MFPSHYAISLSGEPTMYPKLPQLIRYLKTLPETKSIFLVTNGQE
                     PEMIQRLQDEDALPTQLYLSTNAPNEETFQAINKPRYKDSWERWNTTLEMLPKLNTRT
                     VIRMTLIKDYNAKEDLIPEFARMLKRASPHFIEVKSYMHVGRSTNRLDYSNVLEMDEV
                     RHFGSKLAKSSEIFSVMDESIMSRIVVLQNQDRLIDRWISAHANTN"
BASE COUNT         5331 a         3108 c         3159 g         5308 t
ORIGIN      
        1 gaagatagct ataatagaca aaagagatct tttttcatca tgccgaaact ggcatttttt
       61 ctcatttgcg ttttgttggt atcgatgttt gcaaaacaat cgtatggatt ttatgacgtt
      121 gatctaccgg aactaaacac aacatttgat ttaaaagaaa actcaaacat catgaccatt
      181 tctccaacaa tcaaagaaaa tgacattaaa cgatatgttg tttttggctc tggtacgatt
      241 tctgatgtta gatctattgc acaaaacata gtttatcaaa ttaattcaga tcgaggtttt
      301 ttctctgtag gaattttttc acaaactgac atacaagatc tcaaatctaa aggatataca
      361 gtaattgaag attttccatt ggagtttgat tctactccta ttgatagtgt aagagaagca
      421 tcacgaattg gaacaataac tgggtctgat gaagtctctc aaaaatatgg ttttacagga
      481 aaaggagtaa caattggaat cgtagatacc ggaactgatt tttcaaatcc tgatattcaa
      541 cattcgcttg cgcgtgatga aaataatcat ccaataatga ttgatgctga tggtcaagga
      601 ctggttctca ccaatgctac atttattgca aatattaaca aaaaaaatgt aattgtaaat
      661 tattcaaaac ccgtaccaga aaacattacc tcttcagtat acgtgaattc caaaggcgtc
      721 tttttggatc tggcaaaaaa aggaaatggg acaagcgtgc tagtatttaa ttctctattt
      781 cctagtggtg gcactccagt tcttaacgga accatatcta acgatatgaa gattggtaaa
      841 agtaaaactg actatattgt ttcaaaaagc ggaatatatc atctaggagt aatttatcaa
      901 agtgtcatac aaggaccatt tcctaggttg cagcttgttc cagtactcgt tattgattca
      961 aaagttgctg gactctatga ttctgttata cctgacatgt cgacatcttg gaccgattat
     1021 actcgctttg attcaaataa tgctaaacca gtagtgtatg attttgattt tacagacgaa
     1081 attcctatga ctttgggaaa tggaaaagaa ttctttttgt atgattatga caaagacggt
     1141 aaatttgatt atagtgcagg aacgtttggc gcacgagtgc ttgatgtcta tggagtcata
     1201 acgaattcct ctaacattga caaaaaaatt agggcaacaa atggaacctt gttactgccg
     1261 cttgatccgg acggaaatta ttttggtgta atgtatgatt ttgtaggaca tggaacatca
     1321 agtgcagcat ccataacatc aaaggggact gaaaaatatg acatttataa aaattctaca
     1381 aaatatacga taaaaggagt tgcaccatct gcaaagattg ttccaatcaa ggccttgtgg
     1441 tttggtgatg ccgtttatgc ttggttatgg gcagcaggat ttgatcaaaa aaataacaat
     1501 tggaattttg agggaaaacc acgagttgat atcatatcaa acagttgggg catctcaaac
     1561 tttccttcca tacaatctgt atcaggtctt gatattttat cacttgtact gagtgtactt
     1621 gttgtaccaa gttcaatcga cgaagattat ccaggagtac ttgttgttag tagtgcagga
     1681 aatgcaggtc atggttatgg tacattagga cttccaaacg cctcaccata tggtcttagt
     1741 gtaggtgcaa cgacaaataa tgtctatgtc ggatatggtc cattcaaaaa ccaaccaaga
     1801 tttggaaaca gtactttttc ctcaaatgaa atcgtggatt tctcaagcag gggaccgggg
     1861 ataataggtg atccgaaacc tgatctgatg agtattggag catatggttt taccccaact
     1921 agtgtaacaa aaacaagcaa aaacgcaact gatccgtttg caatctttgg tggcactagt
     1981 atggcggcac cacttgtttc tggttctgct gcagtattaa ttgaaagctt gaaacaaaaa
     2041 ggggaacaat atgatccatt tagaattaaa aacatcctaa tgtcaactgc tgccgatctt
     2101 gaaaacgatc ccttctcaca aggcgcaggg cttgttgatc tagaaaatgc agtaaacttt
     2161 gtacaaggaa aaagtggcac ctttattgta cataatgatg caacttatca taatgtaaaa
     2221 aaaattcttg atgttccaat aaacgcaata aactcttctt cttttggtat tgaaagattt
     2281 caattatcta atgccacttt tccagaaacg gcttggtttg gaggcagact atatcctggg
     2341 gaaagaacct ttaccacctt cacaattgaa aatcctacaa acagaacact tgatataaca
     2401 atcacgccac aaaccctgca actaataaaa aaatatcaat atcaaggcaa aactgaagta
     2461 aatctaaaag acccactcct aaacaaatct ggaacattta gaccaaatta cttgcatctt
     2521 aaggatgtcg caaaagacac aggtttgcta tttgagaaag ccaaaccaat accaaatgat
     2581 tcatcattaa tggttctgaa cgtgaatttt ccatttgatc aattcatgaa ttctactgat
     2641 aatatgtacg caaatgacat gaaaatttca tcgctttatc tgtatgactg gaatgacaaa
     2701 aataatgacg gcaacactac atacaacgag cttactctgg taaatcgcgg aggttcatgg
     2761 ggaactgttc aagagatgcg tgtgtcagaa ccaaacacaa aatttgacaa cattcctttg
     2821 gttggtgtat atcctgtacc aacaagatat tcatactgga ttggagacac aaagaaaaat
     2881 tccacttcaa tggattatac agtaaccact agcttttaca aaaaagaaaa ttgggatgca
     2941 gtttgggtaa attataaaca aatacagatt ccacctaata gctctactga acttgttgca
     3001 acacttgttg ctccactaga ctccaagcct ggcgtctatc aaggatttat gactttcaaa
     3061 ggtgattcac atgaagttaa cgcaccagtt tcgtttgcag tcaaaaagaa agttcaagca
     3121 aaagacatgc caactgtgat agaaggttct acaacagatg atgtgctata tggtaatgga
     3181 tacataagcg gagcctttga catgacaaat aggtataacg ctggggattg gaggcagtat
     3241 tattttgatg ttgaagatga aaccataaac tctttagcac ttgacatttc atgggaaaac
     3301 aaaaatacga atctatctgt ttttgtgata gatcctgtgg gacgaattgt acaaactaat
     3361 gtcccgccag gagtactagg tcattttcaa ggatggccaa ctggagattg gcttggtact
     3421 actccattta gcgaaggcgg agggttcttt ccaatcaaaa acaaagatga tacatctact
     3481 gtcttgtttg ctcctataaa ccagactggc acgtattcgc ttttaatgca ttccacctta
     3541 tttggtggcc aatctgttgt agaacctatc acagtcactg ccaagttttc tacaatattg
     3601 ccagatgaaa aagaccctca gatattatct acgattcctg aatttctaaa tagtactcac
     3661 atagttgtgc cagaaataat agaagaaaac cttgatgccc tgacatacta tcttgatgaa
     3721 aataaaattc aaggaattga caaaaaagag cgatctgtac cttccaaact tctaacagaa
     3781 ggtacgcaca cactccgagt agttgcaaaa gatacagttg gacattatgc agaaaaaact
     3841 cttacattta cagttgatga cactcctccg atcatattga tcaaatctcc acaaaatggt
     3901 tccactgtct caaacgtgct taccatagac attgatgtat cagaactaca tatgccagat
     3961 tctgaaggaa tcacagtcat acttccaaat ggcactatat cggacaagac atttgttcag
     4021 tttgatacac gcacaataga aaatggaaaa tatgacatta aagtttcagc aaaagatatg
     4081 gcaggaaatg aaatctctaa aaaaattaca gttaatgttg acaacaacca attgtttgaa
     4141 aatccaataa cgtctcctga tcaaagcttt attcttcttg aaggaatact gattggaatt
     4201 gcgatcgcaa caattgcttt actaatcact ttaaagaaaa tcaaaatttc gaagaaaaac
     4261 tagaacaagg tttatatgca atttttcaaa aacggaagct tagatgtctg aacaggattc
     4321 tagtgccaag ggtacactgt ccagacgaga ttttctaaag cttatgggcg tagcaggaac
     4381 tgcacttaca tttactccgt ttataccttg ggggaaattc atgcccaatc cagatactgc
     4441 ttctttggac aaagccaaag tcattcttcc tgatgataca caggctaacg ttaacacctt
     4501 tccagtaaac catgcagaag taatcacata tccaaaaaca ggagacagag ttttagatga
     4561 agaagccttt agaaagtggc aatttatcag acttccatca gaagttggtg gtgatgcaaa
     4621 ggatgttact gcttttagag cttatagtat ggtgtgttta catttgtggt gtttgtggaa
     4681 atattggccc gaggaaagac gaaggagagg agaatgcccg tgccatggga gtatgtataa
     4741 tgtacttact ggacaagcat ttgcagggcc agcctctctt caagctccac catctaatac
     4801 tcttgcacgg ttaacacttg aagcagataa cgaggggttt ttgtatatct ctccagcgaa
     4861 atggagtgta aatgaaaacg gagttgtagg atatggtcgt ttcattaaag cgtagaaacg
     4921 gtctagtaga tttcctctac tgggtgtggg atggaataga cagaacagta ttcatcggta
     4981 caaagttttc atttcctgcg cgatttgtaa gtccgtttgg attccttggc atgcttacat
     5041 ttgtcacttt cataatcctt ggaatcactg gagcgctctt gatgttctac tatgaaccga
     5101 tacttgacag agcttgggac agtgtagaaa aaataaacaa taacgttcca tttgggtttc
     5161 atctaagaaa tattcactat cacgcatcaa acgcaatggt tcttttggca attcttcaca
     5221 tgtattatca atactttagc ggaagataca agataagaaa cgagatactc tgggtaacag
     5281 gagtcatact tggaacagta acaatacttg aagcctttac aggatatgat ataatattta
     5341 gcgagcgtgc agagcttgca attagcatag ctgcatcact tacaaactcg ataccgattg
     5401 caggaccaac catacgtgat gctatgtttg gttctggatt tgctgacttt gtactaagat
     5461 tctatgctca acacgtattc gtacttccaa ttgtgatgct tggcttgatg gcagttcaca
     5521 tgccaagatt tctagtattt gatgtaccga tggttatggc aatctctgga gccatattga
     5581 ttacgggagg agtatttcct gttgatctag gattcaagtt tgaacctacg gtaccgccag
     5641 gaatcacagt gcctgaatgg tatcttacag gactctatgc atttcttaga acacagtacg
     5701 acaagtttgt aaccggagta gcttggccag ggttgttcat tctagcactg cttatcacgc
     5761 catttattga tagatacaag aaattttctt ggaaagacag accaatggta actgcatttg
     5821 gtatcaccgg aattgcgcaa atcatggtaa ctacatactg gggattctat atctcgccag
     5881 atacaacaat gccacttgtt gaaagactgg tcatagatcc aatattcttc tatctggtaa
     5941 tgatattgct tgtaccacta ggatttggat tctcctatat gatgattaaa atgtcaaaag
     6001 aagcagaaag aaaggcaaag cttgctgcat caaagggacc acaaaaagta gcgcaaataa
     6061 acctctctgc aaagtggata aattggctga tagtcgcact tttaggattc caagtatacc
     6121 taaacattgc tgcatacaac gctgcattgt ctggcatgaa caacttttcg ctctttctta
     6181 cagggttgat cctgcttgtg tttgcaggct tgttccatgt gtatagatac ggaatgaatg
     6241 aggcaaagaa gattccaact ccaccaccag caattgcaaa agaaattcca aaaccaattg
     6301 gtgttgaacc aactacaaaa cttccagaaa aagataaaac atcagattca aaaccatctg
     6361 caccaaaacc aactgctcca gaaataaaaa cgatttctac agtggataca acaaataaaa
     6421 taaatgctga tccgtctgaa attaaaaaat tataaaataa ttaaattttc atcaaatagc
     6481 tttataagac tagcaaaaag caccaagaat tgttgagtac cccaacatca agccatgcat
     6541 atggaatagg aataattgcg atggtaattg ggttggctgt tggaatttca tattaccaac
     6601 tatattatat tccggaactt actgccaaac caaaaatttc agaagaagta cttaatcctg
     6661 ttgaagcaga gaacctcatc aacatgataa aaggctcttc aagtccagaa cagcaagata
     6721 actttgtacc aaaattagta aatatacaac ttggaattga taatcacgtt gtctgggaaa
     6781 atattgatga gaccccacac actgtaacag ctgatgctgg tcatgaatat actgacaagt
     6841 acagtggcaa atttggttcc ctcggtgtag taaaaccagg tgaaaggtac gaattcttat
     6901 ttacagaacc cgaagttgag atagaatatc actgtgaacc gcatccatgg atgaagggta
     6961 aactggtaat tacaaaggcc agattctaaa ccactagttt agcaaataat acgtcacttt
     7021 caatttcttt atgttcttga attatagaga cacgaaactt tacgtcatga gattcggttg
     7081 gtttgaattc tattgctgca gtaaattccg ccttgttatc aggcatatgg tctttgctta
     7141 tgaacagtct agaaagattt tgtgagatgg gtttgtcatt atatgacttg tatactatga
     7201 gctggtttga atttgagatt tgtgtgttta ttacaactcc gtcatatctt cctggatacc
     7261 tgacttggat tttgcccttt atctctgtga atggaattat gtttttattt tcaagtgcca
     7321 attcaacatc cttcatgttg tatgcgtctt attatgaaaa taaataactt gtgaacaaaa
     7381 gcgggcccga agggcttcga tcccttgacc actggattag aagtccagca ctctgtccat
     7441 gctgagctac aggcccaaga attgggcata ggaccaccat attaagagct ttttacaacc
     7501 attttttgtt gtttagttct ctttccattt tgaaaagaaa ttgttgagaa tatcctgcat
     7561 aaggaccaaa atattctaca attttttcat gtaggttcat gtattttttt ttagtaagtg
     7621 atttgcctat gattttctga aatttgtttg cataatagtt ttgtaatatt cttagagtcc
     7681 atctatctat aggaaatgct tcgatctttt caagtgaaaa caaaagtatg caatctgcga
     7741 tcttatctcc tataccatag acttcttcaa gtttttcctt ggcagtcttg taatctgatt
     7801 tttttaatac atcaaaatct atttttccag agacaacagc gcgcgatgca ctctttacat
     7861 attttgctct aaatccaaga ccgcatgata acaaatctct caaactggca ttggcaagtt
     7921 tttcaggttc aggaaaagta aaaaactttc taccacaaaa attaattttt ttaccaaatt
     7981 ttttacatat cttctctaac atttgtttaa tgtttggtat tgatgcattt gaagaacaaa
     8041 taaaagaaat atagcattgg aatggatcct gccgcatcaa tcttaatcca ggaaattgaa
     8101 aagtggtaga tctgactaaa ctatccttac ttatgtctga aagaattttt ttcatattgt
     8161 cattgtctct gaaaaggtca gttggttttt tctgtgaaga aattatcttg aatggattct
     8221 gagttatcat aaacacctct tttccgttta taccgtacca aattccatca atgttttccc
     8281 acaaaaaaac ctgaccgcta ttaatggtat tgtctaaatc tatttttgta atttcttgca
     8341 tttcaaacag tgattgtctc gccagcactt ggagccatgg catcaaatcc aaatttttca
     8401 tgtatttctt cggcaaactt gacacaagag tctttgtccc catgaactgt caaaattttt
     8461 ggatttcctt ttattttctt gatgatttca aaaagaccat ttctatctgc atggcctgag
     8521 aattcaaact gtcttacttc agattctgcc tttacatctc ttccacgtat gttgacacga
     8581 cctgtctcaa gtaattttct gccgggagtt ccttcgcctt ggtatgatac tagtgcgatt
     8641 ccatttttct tatcaagcgc aagctgttgc aaataatata ctgcagagcc accaacaagc
     8701 attccagctg gagatattac tacacacggt tcattcagtg ctttttttcg ttcactttcg
     8761 ccttttatcc acaatgcctg atggattgca tctgcaaaca cctttgggtc gcgaagatat
     8821 tctggataac gaagcattat ttcatttact ttcacagcca ttccatccat gatcactttg
     8881 tgtttaaatt tggcatcttt caagacacat gcaatttctt gagatctttc tactgaaaac
     8941 gacggtacaa aaagagaacc tttcctatct agtacttcat aggcaaattc tatgaatttt
     9001 tcctcggttt cgttacgtgg tgtttgctct gtttgtgaat aggtgctctc tgttattagt
     9061 aaatcgacct cgccaaaatc cagatctgcc tcacgtaaca tgcgtgagcc tcttggattt
     9121 atgtcacctg tgtaatagat tttcttattt cctgattcta caactactgc gcctcctcca
     9181 ataacatgtc cggattctaa aagttcaaac gatgcattac cgcgttttat tttttctcta
     9241 tatcctatct ctttagaacg catcatcatg ttagaaactt ctttcatttc aaacggcaag
     9301 aaattttttc ctagtctgat catgtcttca attaaaagtc ttgaaagatc taatgttggc
     9361 ggtgttgcat acacatcgca tccaccactt acaaaaagaa atggtacata tcctgagtga
     9421 tccagatgtg catgagttat aactactgca ttaatgtctc ggggtttaac atgtaatgga
     9481 tactgctgct cttttcctaa aaatactcca taatctaaaa ggaaatttgc accatcacaa
     9541 ttaacttgga atgccgatcg gcccacctct tgggcggcgc caagaacttt aacctgcaag
     9601 aattcaacat cctttctgtg ggcctttaaa cgtttagcgt ttttgagaca tcattatatc
     9661 atttagataa tttacaaaag acttaattag atcaaataaa gatgatctgt ccatgggtag
     9721 aaccttcgaa caatggtgga atactatccc aaaagacctt cgagataaag ttcgaaaggg
     9781 cgatgaggga aacaagccat tactaaatca gataaattgg gtttgggtac acaatatgat
     9841 gaatcaaaag ggcgatctaa atccatcatc tgctgaatta ctcgactggg ttactagcgg
     9901 tcaaatcgac gctattagac agtccaaaaa gtagttacac atatcaatta ctctttgttt
     9961 tttatttttc aaaagttatt gaaaaaattc tataaccctg ttatggattt tacagatggt
    10021 ttaaatagca accgaaggat gatcatccag aagcaaaaat gccaatagca atacttccag
    10081 acatagatga acaaagatgt ataggctgcg cactatgcgt agagatctgt acttctcttg
    10141 gtcctgatgt actccgtgtc aaacccgtag agggttggaa gagaggtaaa gcatttgtct
    10201 tttatccaga aagatgcatc tcagacggtg catgcatcgg agtttgtcca acaaagtcaa
    10261 tcttttggat gagaccaatg acttacacag ctggtcaacc agtacctctt cacaagaacg
    10321 gtgtattcat aaaaggctgg gcagaagacg ccgcattaag caaataagcg gaatctgttc
    10381 gcaactcttt tttattattt ttagaaccta ctgataggta ttttcttgat tttctttgga
    10441 agtatttttg cgtgattttt tataaattcg tcttttgcca aataaatcgt tttaaagttg
    10501 ttatttgaaa aaaatgtctt ccatgttttt gtaaactctg gaaattctgt ataatcatat
    10561 tgcattcttg gcatgacata atgtgatgca ggatacatgt ctgagatttc aagtgggatt
    10621 attccaagaa atggattgaa ttgacagaac tgaacatctt gagatttttt tataaacttc
    10681 tttctcagtt tgctgtattc cccagaaaga taaaaaggtc tcatggttcc atcaggtatt
    10741 acgacaaggg tgtctttttt tgttttaaat ttacgagcca tcctatgaaa agctgctact
    10801 tccggtctaa attggtcctc ttttgaaaac agaaatactg ccttctcttt gaatctgggt
    10861 gttctatcaa taaaataatc agaattctct acaaaagtgg atatggtttc aaataatttg
    10921 ggatgtgaat gtgccttttt aattacatac tcccatagcc tgccatcgtg tatggcttgc
    10981 tttactctgt ctacttcggc ctttatggca aacagattgt gtagtgcaat cttgtttgca
    11041 cgctcattat tttcaagtgc acgcatttct tttggtgtgt attttacaca gacttcgcaa
    11101 gtacaggaaa aatatgcaat ctcatcaagt ctgacagttc catcttcagt aatgtatctg
    11161 tcatgtcttg catacagcat ataggacgca gaatcaaatg tgtcacagcc aagtgcaacc
    11221 gcaagcggaa ttgtcattgg atgtcccgct ccaaaaagat gtaaaggaat agaatcaggc
    11281 atctgttttt ttgcagtaat tatcattttt gcaaggagct tgtattcata tgattccata
    11341 aattcaactg ggctgccaag tgcaagcatt tcaaatccat actcaacaag ggttttagtt
    11401 gatctcttta caagatcaaa atgttcccca ccctgaattg gtcctgccca gatctgacca
    11461 tttttttcac tttgctctag agtttctttt gatactttca aagtatgttc gacgtatgat
    11521 cttgcctttt ttttagaaag accatatcct gttggtctgt caagtggaat tgcaatgtct
    11581 gtttttattt cctgttcaaa atttgccatg tcttttgctt ctacatctac tttgccgtat
    11641 tcgagtactt gatatccacc ggaatctgtc atcactgctc catcgtaatc tattatgcta
    11701 tgaatgccgc gtttgattgc ctcttgccca taattgttta atgtgatgta tgcgtttgtt
    11761 atgacaagat caaaacccat atcacgcatt ttttttgctg ggatggcttg ctttacggga
    11821 tgaaccacgg gcacatacgc gggagtctca acctcgccat gatttgtgcg taatatgcct
    11881 atccttcccg caaggtcact tttttttatt tcaaacattt tacaatatca tcattcatgc
    11941 gttatttgaa gctagtaggg gaaaaatcgt ctcaagtcat ttttttcaga aataaggctc
    12001 agccaatcca cttcatcttc ttgaaagata tgtttagtga aaagatccgc tatttttttt
    12061 acaactgtag acactgactt gagagtagta tcaatctgaa aagttttatc aggaccaaat
    12121 tcgtttattg catcatataa tgtgacccca agaatttcac tacccaaatt ctcaattgat
    12181 ttctcataac tgtattttct ttttttatat attgaagaga gactatatgg attttttctt
    12241 agaacaactg cgacatcaac ttgttttctt gatacaacat aaggagccaa atgtcccaca
    12301 ataagagaat tttttgtatt tttctttgtg attatttttt ttaatatttt gacatcgaca
    12361 tctagcgttt catttttctt ttcaaagatt ccttcctcaa tggctacttt gtttatgtcg
    12421 attatttcca aattaagatt tttggcaagt tgtttggcaa ctgtatgctt tcctactccg
    12481 ggactcccag ttatcacttt tgacatactc tgttatcaat tcaaagtgga ttaaacgatt
    12541 tctgcaatac taataagagc atggattaga taattggtca tgcaaattgg tttgcttgga
    12601 aagacaaatg ttgggaaatc caccttcttt tcagcggtaa ctgaaaccac agctcagata
    12661 ggaaactatc catttacaac tatagaacca aatgttggaa tatcatacgt aaaatcagag
    12721 tgtgcatgta aacactttgg aataaatcac aaacatcctt tatgcattaa tggaacaagg
    12781 ttcattccaa taaaattaat tgatgtagct ggtcttgttc caggagcaca tgagggcaaa
    12841 ggacttggta acaagttttt ggatgacgcg cgacaggcag aagtactaat tcatgttgtc
    12901 gacatctctg gttctacaga cattcaaggt cattcagttc ctgtaggaac gcatgatcca
    12961 atggaggatg taaagtttgt tgaggaagaa tttgatcaat ggatgaaaca aattctgcag
    13021 cgcgaatggc agaaattaac aagagaaatg gaaagcaaag gtgcaaaact tgtccaagca
    13081 atagcacaac gattcagtgg actgggcatc aaagaatatg atgtagagat tgtgatccaa
    13141 catctgaatc tgcaaacaag aaaacctgca gactggaaag attctgattt gtttgcattt
    13201 gtaaaagaac tgagaaaaaa tacgaggcca gtacttattg cagcaaataa agctgatttg
    13261 tgccacgatt tgagcatatt tgaaaagcta agaaaagata gattggctat agcatgtagc
    13321 gcagagactg aactgctttt aaaaaaagca tcaaaaggag gtctaattga ttatctgcca
    13381 ggagacaaga cttttaaaat aaaagacggc gttagtctga atcctcaaca acaaaaagca
    13441 ctcgatcttg ttaaaatggt attctcaaaa attgaaacta ctggggtgca gcaagctctg
    13501 aacgcagcct gttttgaact tcttaaacta attgtggtgt atcctgtaga agatgagaat
    13561 aaactttcaa acaaagacgg tgaggtgttg cctgatgcta ggcttttacc gccaagttct
    13621 actgccaaag atcttgctgc cacgatacat gcagacttgg caaagggatt cttgcatgcc
    13681 gtagacgtaa agacaaaaca acgaattggt gcccagcatc aattaaaaaa tggagatgta
    13741 ataaaaatag tctcaacact aagtagggga taacatgtta ggtcttggaa ttgaaagtac
    13801 agcacataca ttttcatgcg ctatattaga aaaaaccggt aagcatggaa aaattctttc
    13861 tgatgtcaga aagatctatc gtccacctga tggtgaggga attcacccaa gggaagcttc
    13921 acgacaccat gcagaaaatt ctccaattgt actttctgag tgtttacaaa acgccgatgt
    13981 caaaataact gatcttgata taatatcata cgcagctggt cctggacttg gaccttgttt
    14041 gagaataggt gctgttgtag ctcgttctct tgcatcttat tataacattc ctgtgtatcc
    14101 agtaaatcat gccattggtc acattgagct tggcaagatg ttgactggag caaaagatcc
    14161 tttggtgttg cttgtatcag gaggacatac aatgatcttg gcgtttttat caaaaaaatg
    14221 gagagtcttt ggagagacat tagacattac acttggccaa ctgatagatc aacttgggag
    14281 atctgctgga tttgcatctc catgcggaaa caaaattgaa gagcttgctt tgaaatctaa
    14341 caactatctt cctcttccat atgtagtgaa aggaaacgat gtgtcatttt ctggtttact
    14401 ttctgcaaca aagcatgtgt tatcaaagag tcttgaggat gcatgttttt cattacaaga
    14461 aactgcattt gcaatgattg gtgaggcagt agagcgtgcg ctttcattta caagaaaaaa
    14521 agaactgctt attgtaggtg gtgttgctgc aaatagaagg ctatctgaga ttcttacaag
    14581 cgtatgcaaa agacaaggct gtaaattctt tgtttctcca aaacaatatg ctggtgactg
    14641 tggctctcaa atctcatgga ctgggttgtt agaatcttca tacaaatctg gtgtatcgct
    14701 agatgaaaca tttgtaaaac aatcatggcg attggatact gttgaagtgc cttactagtt
    14761 cttgttttgt gctattgttt cctgtagtgt ttcaagccac tttttggtgt tatagactcc
    14821 aaacttgtag gtcttttctc catctggtgt ctttgccttt agccaaattt ttttaattag
    14881 ccaaccctct cttccaactg atatgatttg atttagaggt acgtcaatca ctgtatcacc
    14941 aatttttttt gtaaagtcag ctattctgcc tttagtttta tcaaaagcaa ctctcatgtt
    15001 tgttagtgtg agaattccgc tacccatgga gtcttcagaa caatcttctt gaatgatctt
    15061 tttttctgaa tcttccataa ctaggtttta atctctacct acaagttata actctaatga
    15121 aattactaaa aaaaggcgct gaagctgata tctatcttac tttgtggaat ggaaaaaaat
    15181 caatcctcaa aattagagaa aaaaaagact atcgaaactc aatacttgat aaaagaatta
    15241 gagagcacag gaccatacgt gaatcaagta tcatatctga tgtaaagtca tttggaatac
    15301 cttctccaat tgtatattgt gttgacacaa aagcatgtag aatatacatg caacacatat
    15361 ccggaaatct tgtacgagat ctaccaaaat ctagtgtgat taaaacatgt agggacattg
    15421 gtaaaatagt aggcacatta cacaacaatg gaattatgca tggtgatctt accacttcga
    15481 actttattgt attgaatgat aaagtatttg taattgattt tggacttgcc cagagaacag
    15541 aaaaaattga ggatcatgca atagatttga ggcttttcaa agaaatacta aacagtgcac
    15601 acgcggatat tatggaaaaa tcttgggctg gttttctaga gggatacaaa aaaattgtag
    15661 gggaatcaag acagaacaga gtcctctcac aggtttcaat tatagaagga aggggaagat
    15721 acgcaaacgt tgtctagtat attatttgca tcttcaaaca aaaacaaata cgaagaagcc
    15781 aaagatgtcc tatccaaatt tggagttgat ttgaaatttt tcaagtgcaa tctggaagaa
    15841 attcaggcag acacacttga agaaattgca ttacacaagg cacaaaatgc attcttacag
    15901 tgctcacgtc ctgttatagt tgaagatgat gggcttttca taaattctct aaatgggttt
    15961 cccggcccat attcgtcatt cgcattcaag actcttggaa atgcaggaat cttaaagtta
    16021 ttaaaatcac aaagaaaggc tacatttcaa tctgttatag catactgtga atcaacagaa
    16081 gatgttttat tttttaaagc ggttgtaaat ggcaagattg caagaaaatt aatgggaaaa
    16141 agatggggat acgatccaat ctttattcca gagggacaaa caaagacata cgcacaacta
    16201 gaaaacaaaa attttgtttc acacagatat cttgcactaa aaaaatttgc taattggtat
    16261 ttgcatgagc ggaaatccaa cgatcgatga gtctgtcttg gttttgaagt actactatcc
    16321 tggacatgat gctttcatcc atgactgaaa agatctcgct actctttgca agcttgctgc
    16381 caaagtgtct gacttcatcc atttctaaca catttgaata gtcaagcctg ttagtagaac
    16441 gccctacgtg catgtatgat tttacttcta tgaagtgtgg actagcgcgt ttgagcatac
    16501 gagcaaattc cggaatcaaa tcctctttag cattatagtc tttgatcaaa gtcattctaa
    16561 ttacggttct ggtgtttaac tttggaagca tttcaagagt agtattccat ctttcccatg
    16621 aatccttgta tcttggtttg ttgattgcct ggaatgtttc ttcatttggt gcatttgtag
    16681 aaaggtacaa ttgtgtaggc aatgcatctt catcttgtaa tctctgtatc atttctggtt
    16741 cttgtccatt tgtaactaga aagattgatt ttgtttcagg caatgttttc aaatatctta
    16801 tcagctgagg aagctttgga tacatggtgg gttcaccaga aagcgatata gcataatggc
    16861 ttggaaacaa ttattcatca agtttcttct tgtcctgtct tggatc
//
LOCUS       DATIDL010000083         4723 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrosopumilaceae archaeon isolate SMAG_U16342
            k141_2929713, whole genome shotgun sequence.
ACCESSION   DATIDL010000083 DATIDL010000000
VERSION     DATIDL010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935836
            Sequence Read Archive: SRR25158326
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosopumilaceae archaeon (soil metagenome)
  ORGANISM  Nitrosopumilaceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrosopumilales;
            Nitrosopumilaceae.
REFERENCE   1  (bases 1 to 4723)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4723)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/04/2024 05:09:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,520
            CDSs (total)                 :: 1,474
            Genes (coding)               :: 1,474
            CDSs (with protein)          :: 1,474
            Genes (RNA)                  :: 46
            rRNAs                        :: 1 (5S)
            complete rRNAs               :: 1 (5S)
            tRNAs                        :: 43
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4723
                     /organism="Nitrosopumilaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2929713"
                     /isolate="SMAG_U16342"
                     /isolation_source="soil"
                     /db_xref="taxon:2202732"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..535
                     /locus_tag="VLB45_03635"
     CDS             <1..535
                     /locus_tag="VLB45_03635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="exonuclease SbcC"
                     /protein_id="HSD04827.1"
                     /translation="KIKFEIDTQFSKISRPLGKYSYVSSLDKSLKKIMEDLIAEPSEM
                     IMNENKDSIVQILQSVVKGVVAGNVSVKDSQKAVEQIEETIQKLGEFIRIKEKFSQKL
                     SSLENSLSIFDIKKLEENEKLLSKTIDEKSHEESKLSSLEKEIEENTKTIPQLASDIE
                     KKLSNILLAKVNLKIQD"
     gene            540..938
                     /locus_tag="VLB45_03640"
     CDS             540..938
                     /locus_tag="VLB45_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007550307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mov34/MPN/PAD-1 family protein"
                     /protein_id="HSD04828.1"
                     /translation="MIFKKTNFQRTIFLKKDARDGILSYCKMNHPNEGILILRGKSKK
                     GEVTIDRLVIPPFSHSGPTFAGFPHSFLPFDPSYVGTVHSHPTGSARPSVTDLHNFFG
                     LVSIIVKSPYEDEDIFAYDRDGNSIPITIV"
     gene            986..1285
                     /locus_tag="VLB45_03645"
     CDS             986..1285
                     /locus_tag="VLB45_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016939620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04829.1"
                     /translation="MSAINYKSYLIFLFASLAVSIGLQMLLPWPYGLIAALAIFIAFP
                     LFLRRRAMKRMGSFGEGGGFFGQSQGVKYICLTCNNKYKGGSCPRCGSKMKRADF"
     gene            1309..1626
                     /locus_tag="VLB45_03650"
     CDS             1309..1626
                     /locus_tag="VLB45_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04830.1"
                     /translation="MTLEELRKKALFQNSIDTWIMACEETNFDWYSIENYKKFIDHLT
                     KNGLRMQKFPLCVKETGGMYERGRDKTQFAESLANDTDPNAAAYTIRLNDETIKIIRQ
                     FKP"
     gene            complement(1623..2975)
                     /locus_tag="VLB45_03655"
     CDS             complement(1623..2975)
                     /locus_tag="VLB45_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSD04831.1"
                     /translation="MSNQLWKEFSKNKVGMVGIGILVFLFGISAFAVITIPVETFKEW
                     NNPTSWITYPKSSLPSWVNYFLSEKIPEHKILNPYRSSQVSGDVHVVSESFVVDYQYD
                     GFPSDFIYEYSAKYAGAPLLQISVMRPDGLEMGLVSMSIPKSKTETEYHELVFSTDDS
                     MKKNIRKHTGNFGFQTDTLSSEDIVFSDKNTNNILKGRYIFKVNFYDADSKISIDNSR
                     LILGGKVYGLVGTDDLRRDLMIGLMWGTPLALFIGIAVAVGSVVIGLIYGVFAGYKGK
                     RTDETMMRFNDVIYALPALPLLIILAVTVGNTIFLIVGFLMIFGWVGVAKVSRSMALQ
                     IKTLQYVEASKIMGQKDIKIIFKHILPQLLPYTFASIAISVPAAITTEAGLSFLGLGD
                     PTFPTWGQILHDANSFGAAARGLWWWILPPGIMIALTGLAFVFIGNAMDTIANPRLKR
                     "
     gene            complement(2978..4030)
                     /locus_tag="VLB45_03660"
     CDS             complement(2978..4030)
                     /locus_tag="VLB45_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016939754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSD04832.1"
                     /translation="MSFKKYLLTRAITMFGVLMATLLVTVALVGSNMDSILKQGIAID
                     IRQQITENSSLVGSFKTPEDLELFITSQVDDRIKSLGLDQPWHSPQRLGLTVYKILTL
                     DFGHATFLTSDSGSSDVKDILLEKLPRTILLFTTATVIISLIGIFLGAFSGNKINSKI
                     DRLTSTFAVVSSSFPVWWIGMLMIFVFAFAYQIFPARATPLIPATDPGYIGALLYHMT
                     LPLVTIVLIGFGSWAYLVRNFLIGVLQEDFIMAKKAIGIPEKKILYRHALKNAAPPIV
                     TVLALSLSGSLGGAIITEAVFDWPGMGRLYYEAITVLDLPVIIGATYVLTAFFLASVF
                     VADILYGFFDPRVRTG"
     gene            complement(4065..>4723)
                     /locus_tag="VLB45_03665"
     CDS             complement(4065..>4723)
                     /locus_tag="VLB45_03665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04833.1"
                     /translation="PVALEQFESNPDYYKSRYDATISWIEKYNHAIISNGPFYLESYS
                     PEARTITIRSFDDPTYPFEAGHWKEFENVNLAKINNAEVPSVVSVGEKITISVSTSEN
                     SSLYYYFTNADGKVVDSGMEKSFDGKIDIILSEEETTLFSIGANDLMLFAVSDSALRP
                     DIFQTSFLAVTDKTQMPTLSLDVGSEALYVEPNYLLIFGVVVGIVAIILVLKRKRKQS
                     "
BASE COUNT         1546 a          949 c          877 g         1351 t
ORIGIN      
        1 caagataaag tttgagatag atacacagtt ttcaaagata tcacgaccgc ttggaaagta
       61 cagctatgtc tcatcacttg ataaatcact caaaaagatc atggaagacc tcattgcaga
      121 gccttcggag atgatcatga atgaaaataa agattcaatc gtacaaatat tacagtcagt
      181 agtaaaaggc gttgttgcag ggaatgtatc tgtaaaagac tcacaaaaag cagtcgaaca
      241 aatcgaagaa acaatacaaa aactaggcga gtttattcga atcaaagaaa aattctcaca
      301 aaaactatcc tctttggaaa acagtttgag tatttttgac ataaagaaat tagaagaaaa
      361 tgagaaactg ctttccaaga caatagatga aaaatctcat gaagagtcaa aactatcatc
      421 attagaaaaa gaaattgaag aaaatacaaa gacaatacca cagcttgcgt cagatataga
      481 gaaaaagctg tcaaacattc ttttagctaa agtaaattta aagatccaag attaggatta
      541 tgattttcaa aaaaactaac tttcagcgta caattttctt aaaaaaagat gcccgggatg
      601 ggatcttgtc gtattgcaag atgaatcatc caaatgaagg aatcctaatt cttcgtggaa
      661 aatcaaaaaa aggtgaagtt actatagaca gattggtcat tcccccattt tctcacagtg
      721 gacctacttt tgcaggattc ccacattcct ttttgccatt tgatccgagc tatgtaggaa
      781 cagttcattc acatccaaca ggctctgctc gtccgtctgt cacagatctg cataattttt
      841 ttggtcttgt ttcaataatt gtaaaatcac catatgaaga tgaagacata tttgcctacg
      901 atagagatgg aaacagtatt ccaataacga tagtttagga aaattatgac aaaaacttat
      961 aagcttttta caagcaaaac aagtattgtc agcaataaat tacaagtcat atttgatatt
     1021 cctgtttgca tctcttgcag ttagcattgg cttacaaatg ctattaccat ggccatatgg
     1081 actgattgca gctttggcaa ttttcatagc gttcccactc ttccttcgaa ggcgtgcaat
     1141 gaaaagaatg ggaagtttcg gagagggtgg aggatttttt ggtcaaagtc aaggagtaaa
     1201 atacatctgt ctgacatgca acaacaaata caaaggcggc tcttgtccaa gatgcggctc
     1261 taagatgaaa agagcagact tttagtaagc aatacttaaa aaaacatcat gacacttgag
     1321 gagcttcgaa aaaaagcctt atttcaaaat tcgatagata catggataat ggcttgtgag
     1381 gaaaccaact ttgattggta ttctattgaa aattacaaaa agttcataga tcatcttact
     1441 aaaaatggtc ttagaatgca aaaatttccg ctctgtgtaa aagagacagg tggaatgtat
     1501 gaaagaggta gagacaagac acaatttgca gaatcactcg caaatgatac agatccaaat
     1561 gctgcggcat acacaataag actaaacgat gaaacaatca aaataattcg tcagttcaag
     1621 ccctagcgct ttagtctagg attggctatt gtatccatgg cattaccaat gaacacaaac
     1681 gcaagacccg ttagcgcaat cattattcca ggaggcaaga tccaccacca cagtccgcgt
     1741 gctgctgcac caaacgaatt tgcatcatgc agtatctgac cccatgttgg aaacgtagga
     1801 tctcccagtc ccaagaagct aagacctgcc tctgttgtta tggctgcagg cacagagatt
     1861 gcaatgctgg caaaagtata cggcaaaagc tgcggcaaaa tgtgtttaaa aattatttta
     1921 atgtcttttt gtcccataat ctttgacgcc tcgacatact gtagtgtttt gatttgtagt
     1981 gccatgcttc ttgaaacttt tgctacaccg acccaaccaa aaatcatcaa aaatccaaca
     2041 atcaaaaata tggtattgcc cacagtcaca gccagaatta tcaaaagcgg cagtgcagga
     2101 agtgcgtata tcacatcatt aaagcgcatc attgtttcat ctgttctttt tcctttgtag
     2161 cctgcaaaga ctccataaat caaaccaatt acaacagaac caacagccac tgcaattcct
     2221 atgaacaatg caagtggagt gccccacatc agaccaatca ttaaatcacg tctaagatcg
     2281 tcagttccca caagcccata gaccttgccc ccaaggatca atcttgaatt atctatcgat
     2341 atttttgaat cagcatcata aaaatttact ttgaatatgt atcgtccttt caatatgttg
     2401 ttggtatttt tgtcagaaaa aacaatatct tctgaagaaa gcgtgtcagt ttgaaaccca
     2461 aaattaccag tatgttttct gatgttcttt ttcatggaat catctgttga aaacaccagc
     2521 tcatgatatt cagtctcagt ttttgatttt ggaatagaca tagaaacaag gcccatttcc
     2581 aaaccgtctg gacgcatcac tgagatttgt aacaatggtg cgccggcata ttttgcagaa
     2641 tattcataga taaaatcgct aggaaatcca tcatactgat agtcgactac aaagctttct
     2701 gagacaacat gtacatctcc agacacttga gacgatctgt acggatttag tatcttgtgt
     2761 tctggaattt tttctgagag aaaataattc acccaagatg ggagcgatga tttcggatag
     2821 gttatccagc ttgttggatt attccattct ttgaaggtct ctacaggaat tgtaattacg
     2881 gcaaatgcag atattccaaa aagaaatacc agtattccga tccccaccat tccaacttta
     2941 ttttttgaaa actctttcca gagttggttt gacatactca ccctgttctc acccttggat
     3001 caaaaaatcc atacaagata tcagctacaa acacactagc aagaaaaaat gcagtcaaga
     3061 cataggttgc gccaattatc acaggcaggt caagaacagt tattgcttcg tagtatagtc
     3121 gccccattcc aggccaatca aacactgctt ctgttattat ggcgccacca agcgagcctg
     3181 aaagactcag tgccagaacc gtgactatgg gaggagccgc gttcttaagc gcatgtcggt
     3241 atagaatttt tttctcagga attcctatgg ctttttttgc catgataaaa tcctcctgca
     3301 agactccaat cagaaagttt cttacaagat atgcccatga gccaaaacct atcagaacta
     3361 ttgtaacaag tgggagtgtc atgtgatata gtaatgcacc gatataccca ggatctgttg
     3421 ctgggatcag aggagttgcc ctagctggaa atatctgata agcaaacgca aaaacaaaga
     3481 tcatcagcat gcctatccac caaactggaa agctgctgct tactactgca aaagtagagg
     3541 tcagtctatc aatttttgag ttgatcttgt tgcctgaaaa tgctccaaga aagattccaa
     3601 tgagagagat tatcactgtt gcagtagtaa aaagcagaat tgtgcgaggc aatttctcaa
     3661 gcaggatgtc ttttacatca gacgaaccag aatcgcttgt aagaaacgtc gcatgtccaa
     3721 aatcaagtgt aagtattttg taaactgtaa gacccagtct ttgcggcgaa tgccacggtt
     3781 gatcaagtcc caatgacttt attctgtcat caacttgcga cgtgataaaa agttccaaat
     3841 cttcgggagt cttgaagctg ccaacaagag acgagttctc agttatttgc tggcgtatat
     3901 ctatcgcaat gccttgtttt aggatgctgt ccatgttgga gcctacaagt gctaccgtaa
     3961 ccaaaagcgt agccatcaaa acaccaaaca ttgttatcgc tcttgtcaga aggtattttt
     4021 tgaaactcat tttgctttca ttattccagt ttggttaata acatctagga ttgtttcctt
     4081 tttcgtttca gtacaagtat tattgctact attccaacca caactccaaa tatcaacaga
     4141 tagtttggtt ccacatacag tgcttcagag cctacgtcaa gactcaaagt aggcatttgt
     4201 gttttatcag taacagccaa aaagctggtt tgaaaaatgt ctggtcttaa tgcagagtcc
     4261 gagactgcaa atagcatcag atcatttgcg cctatactga aaagcgtagt ttcctcttct
     4321 gatagaatga tatctatttt tccatcaaat gacttttcca tgccagaatc caccaccttg
     4381 ccgtcagcat ttgtaaagta atagtaaagt gaagagtttt cacttgtaga gactgagatg
     4441 gttatctttt ccccaacaga tacgacgctt ggcacctcgg cattgtttat ctttgcaagg
     4501 tttacattct caaattcttt ccagtggcct gcttcaaacg ggtatgtagg atcatcaaaa
     4561 gaccgtatcg taatggttct tgcctcaggc gaataactct caaggtaaaa cggaccattg
     4621 cttattatgg catggttgta cttttctatc caagatattg tcgcgtcgta tcttgatttg
     4681 tagtaatccg gatttgattc aaactgttct agtgcaacag gaa
//
LOCUS       DATIDL010000084        12102 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrosopumilaceae archaeon isolate SMAG_U16342
            k141_1920657, whole genome shotgun sequence.
ACCESSION   DATIDL010000084 DATIDL010000000
VERSION     DATIDL010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935836
            Sequence Read Archive: SRR25158326
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosopumilaceae archaeon (soil metagenome)
  ORGANISM  Nitrosopumilaceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrosopumilales;
            Nitrosopumilaceae.
REFERENCE   1  (bases 1 to 12102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/04/2024 05:09:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,520
            CDSs (total)                 :: 1,474
            Genes (coding)               :: 1,474
            CDSs (with protein)          :: 1,474
            Genes (RNA)                  :: 46
            rRNAs                        :: 1 (5S)
            complete rRNAs               :: 1 (5S)
            tRNAs                        :: 43
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12102
                     /organism="Nitrosopumilaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1920657"
                     /isolate="SMAG_U16342"
                     /isolation_source="soil"
                     /db_xref="taxon:2202732"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..734
                     /locus_tag="VLB45_03670"
     CDS             <1..734
                     /locus_tag="VLB45_03670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04834.1"
                     /translation="SVFIHVDDKINKELETSGVVSGKGIVSAVYTITKQDTSYLFEGL
                     STILIPSQIRNSGGFLDVAKKISEEPSSSMTFSIIPQGSSSLYQLQVSIDKPLEPNLA
                     TISPLEFFETEKLQRSNYFSSGFFPLNSIVQVVVLSNQTMKVAGHASELVPILEKDGE
                     KYPSDFTRNGWLFDPDSGDQIVGKYLFGKTTEVSKNDLIFNVGGLTQSTPTNDDSIYV
                     LIGVGIAAAIAIFWYLRKPKTKSEK"
     gene            complement(723..1274)
                     /locus_tag="VLB45_03675"
     CDS             complement(723..1274)
                     /locus_tag="VLB45_03675"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007550991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006596 - polyamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase, pyruvoyl-dependent"
                     /protein_id="HSD04835.1"
                     /translation="MLDLVAKKLFLTRGKGVHEDQLTSFEYALRDAGIAGTNLVLISS
                     IFPPNAKLVSREEGLKLIRPGSVQFVIYARQQSNEPHRLMAASVGIAQPTDKNRYGYL
                     SEYHSFGETEKEAGDYAEDIAAQMLASSLGIKFDINKSWDEKRKQWKISGEIYKTRNI
                     TQSAVGDAKGRWTTVFAAAVLLL"
     gene            complement(1334..1831)
                     /locus_tag="VLB45_03680"
     CDS             complement(1334..1831)
                     /locus_tag="VLB45_03680"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HSD04836.1"
                     /translation="MQNSIKLHVVLQIMQEGEIASDFELPANDGSLVNLSSYRGQKNV
                     VLCFYPKNHLFACPSKKVFKMAESMILAHPQILASGTVLFAISVDTVEDQKKFVEEYK
                     IPYLHLSDTKKVACKTYAGLNLAGLAKRSTFIINKEGKVVKIFRDIDVDSHGQKILDV
                     LKNLK"
     gene            complement(1851..2054)
                     /locus_tag="VLB45_03685"
     CDS             complement(1851..2054)
                     /locus_tag="VLB45_03685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04837.1"
                     /translation="MKKIPPTKLKVMMFAFLGTGVWGIILGNFILFSSYVTMLGVVNL
                     ALGGFVGYLFLNPKPEKEKKEKK"
     gene            2135..2431
                     /locus_tag="VLB45_03690"
     CDS             2135..2431
                     /locus_tag="VLB45_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007401902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HSD04838.1"
                     /translation="MRIVTVGSRVFVTSFQLAGVQGTIVESPEKALVEITKLMNDSTI
                     GLVLVSDDISKTIGNKLTELRAKKSTPLVFELPSPGSQKGEVDYRLLLKQILGV"
     gene            2477..3505
                     /locus_tag="VLB45_03695"
     CDS             2477..3505
                     /locus_tag="VLB45_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent dehydrogenase"
                     /protein_id="HSD04839.1"
                     /translation="MKDPSQVEIRTIDKPSISNGDIMVRMQACGVCGSDLEKIYGQYS
                     QPSMRLGHEPAGIVSEVGKNVTEFKKGDRVFVHHHVPCYSCHFCLHGNETMCKKYSET
                     NLLPCGLAEEFVVPEWNVSHGGVIKIPDLMSFEEAAMIEPLACCVRSWNKIQVKHDDS
                     AAIFGVGPTGMMHVMLCKVYGLRDVFCLDVNEFRLDFAKKFGITEAIKSTDPNANEKI
                     LSKTENRGVDIAIVATGNLNAITQAIEFVRKGGTIILFGVPTKDAKLSVDMSKVYSKE
                     ITIIPSYAASDKDTLNAFKLIQEKKTDVKKLITHKFDLSDSHKALEYAHHGNDSMKII
                     ITNSETLN"
     gene            3540..4361
                     /gene="lsrF"
                     /locus_tag="VLB45_03700"
     CDS             3540..4361
                     /gene="lsrF"
                     /locus_tag="VLB45_03700"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HSD04840.1"
                     /translation="MDWGMKNRLARIIKPETGRGVMLAVDHGYFLGPTERLEVPRKTI
                     QPLLPYADSLMLTRGVLRTSVDANFPVPVVLRVSGGSSIIGKDLSNEKITTSIKEAIK
                     LNVTALALSIFVGAQYEHESLVSLGKLVDEGEEYGIPVLAVTAVGKELEKRDARYLSL
                     ACRIAAEFGAHLVKTYYCENFEKVVNSCPIPVIIAGGPKLANELDALQLTYDAIQAGA
                     VGVDMGRNIWQSDYPVSMIKSVRAIVHENATVKEAHELFTKLKNTEQSAKTATSV"
     gene            complement(4358..4762)
                     /locus_tag="VLB45_03705"
     CDS             complement(4358..4762)
                     /locus_tag="VLB45_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HSD04841.1"
                     /translation="MSETSEILEIIGHFFEVGKTKNLAPLRAIQLDDPRFSSYSDVPP
                     LDLKDYATTIALQELRFVSISDYDFEIKNQRVDVFGDSAVVTFQLKQSGVLVDNYSFR
                     GQHLSMESRATFVLVRSPRWKIVHIHMTKISD"
     gene            5045..6733
                     /gene="ilvB"
                     /locus_tag="VLB45_03710"
     CDS             5045..6733
                     /gene="ilvB"
                     /locus_tag="VLB45_03710"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007401899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HSD04842.1"
                     /translation="MEKMSGARSLMVALEREGVEIAFGLPGGANLPIYDELYKSNIRH
                     ILVRHEQSAAHMADGFGRVSRRPGVCFATSGPGATNIVTGIATAQADSAPMVAITGQV
                     PLKMIGRDAFQESDIIGISNPIVKYAFQPTTPAEIPEVVKKAFYISSTGRPGPVLIDI
                     PKDVQQNEAEIPFPAEIKVRGYHPWVDPDVQQIERAIDMLLSAERPIILAGGGVIISG
                     AFAELQSIAELLMIPVVTTFKGKGSFPENHPLSLGPIGMHGHAEANKMMTEADCVLAA
                     GSRFSDRSVGTFEEFEKRLKIIHMDVDPAEIGKNQTTTVAVVGDIKASLRIMVKMLIQ
                     KAIKRNGEYPWLKRVKETKEYYKENLKIHEHPLAAAKVLRKLREVLPHQSIVTTEVGQ
                     HQMWASLFFDVIHPGTFFSSTGLGTMGWGFPAAIGAKAAKPGVPVVDIAGEGSFNMTE
                     NSLAVSVLDNLPVIVFVMNNFSLGMVAQWQRTFYDRRMMAIDLKNCPDYVKIAEAYGA
                     QGIRAQSLDEIGNAVKTALKSDVATVIDIPIDPQEDVLPFVAPGTPLKDMILPS"
     gene            6743..7234
                     /gene="ilvN"
                     /locus_tag="VLB45_03715"
     CDS             6743..7234
                     /gene="ilvN"
                     /locus_tag="VLB45_03715"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008299418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HSD04843.1"
                     /translation="MWNIISVLVENKPGILFKITNLFRSRNYNIESISVGITENPEFS
                     KMTITTSADEKQVIQIIKQLDKMIDTIEVKRLDEKMSVYRELVLMKIKVTKPSDSMEI
                     MNLANAFECKVHDVRKTSIMVEITATPDRINAFQDLVRGYGILDIARTGVCALPRGGL
                     NES"
     gene            7224..8741
                     /locus_tag="VLB45_03720"
     CDS             7224..8741
                     /locus_tag="VLB45_03720"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HSD04844.1"
                     /translation="MKVRIFDTTLRDGEQTPGIALAPEQKLNIAKKLDALGVDAIEAG
                     FPIISEGEMSAIKMITSANLRAEICGLARTDKKDIDAAVNSGLKYIHTFIATSDIHLQ
                     YKLKLTREQALVKAIEAVEYGKSRGLQVEFSAEDATRTDREFLKKVFKAVAEAGADRI
                     DIPDTVGYSTPEYMAKITKDAVEASKLPVSVHCHNDFGLAVANAISGIQAGAKCAHVT
                     INGIGERAGNASLEEFVMALQCLQFDQKFETNINTKLLYETSKYVSSVVGVHVQPNKA
                     IVGENAFGHESGIHTHGVLSNPLTYEPISPELVGRTRWFQVGKHAGAHGVSAMLEEYG
                     IQPTKEQVHEILENVKKLGDQGKHVTDVELLSIAGEVMKQPGLRRLVHLTGFSVSTGI
                     GNMPYAFVKLNIEGKDFIGTDYGVGPVDASINAIQKITGEIAKVRIKDYRLDSIAGGS
                     DALCEVTIKVEDAYGNLASAKSIGEDIVITSVQAMIDGINRIMLKNILKQKNLGN"
     gene            8742..9758
                     /locus_tag="VLB45_03725"
     CDS             8742..9758
                     /locus_tag="VLB45_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HSD04845.1"
                     /translation="MYKISLITGDGIGPELSESVVSVLDTINDKLGIKLQIEKLEAGD
                     KALAKTGKALPESTLESIRKSDACLKAPVGECAADVIVVLRRILNLYANIRPAKSYPN
                     MPALKDNIDLVIVRENTEDLYTGMEFEIGNTAIALRTISEEASKRIAKYAFLTATQRN
                     QKNKVTCVHKSNVMRKTDGLFARACNQVAKEFPKIQFEQMYIDACAMNLIRQPEAFDV
                     IVTTNLFGDILSDEAAQVVGGLGMAPAANIGDDYALFEPVHGAAFDIAGKQAANPSSF
                     ILSAKMMLEWLATKNNDQKCFDVAKKIESAVYGVVKEGAKTKDIGGNKTTLEFTQHVI
                     SKMI"
     gene            complement(9755..10093)
                     /locus_tag="VLB45_03730"
     CDS             complement(9755..10093)
                     /locus_tag="VLB45_03730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04846.1"
                     /translation="MQQCDFCGSQFGERKCYFCERHCCTTCMTDDRSRCKRCYIDKRK
                     LGWKIFKRNKVLLGFVVFVWAYAVFPIPLIPGLDPTFYWITFIAALLFMIPAGFMMFF
                     WSRNPPSSDI"
     gene            complement(10127..11416)
                     /gene="aspS"
                     /locus_tag="VLB45_03735"
     CDS             complement(10127..11416)
                     /gene="aspS"
                     /locus_tag="VLB45_03735"
                     /EC_number="6.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA(Asn) ligase"
                     /protein_id="HSD04847.1"
                     /translation="MDFKRTHYIGNLSPSMEGTKVIVGGWVEDVRELGKLTFLTLRDV
                     TGVAQIVLVGEDSLKQIKGLTRQSVIKVTGTIQKSKARDFPFEVKSERVDILTVAVQP
                     LPIDPIGRLESNIDNRLNARALDLRNQKTAAIFKMRHHVLASIRKTLTEKSFIEVNTP
                     KLIGSASEGGANLFSLKYFEKQAYLAQSPQLYKEQLTIGLERIFEIASYYRAEKSHTV
                     RHLTEFTSVDIESAFMDYSDVMDILESVVVNVFEYVMQNCASEQKSLGIEIKKPSVPF
                     ERISYKKAIEELGRDGIKLEVGDDLQDAHLRKLGERHPGFYFLIDWPMKLKPFYIHEK
                     DDDPELSRSFDLQYGYLELSSGGTRLHDPIKLKTRLQEQGLDPHQFEDHLKAFDWGMP
                     PHSGWGLGLDRLMSVLTQIDNVREVVLYPRDPERLFP"
     gene            11611..>12102
                     /locus_tag="VLB45_03740"
     CDS             11611..>12102
                     /locus_tag="VLB45_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HSD04848.1"
                     /translation="MTFISVENLKTHYETENGTVHAVDDVSFSLEKGESVGIAGESAC
                     GKSTLGLSIMRILQGGKIVSGNILLEDKSILRMSDSEFSKKHRWKDISMVFQGAMNSL
                     DPVFTIKQQFIEVLKQHHYEGNYDDTISEMVNSVGLDVSVLKKYPHELSGGMKQRIVI
                     AMAL"
BASE COUNT         4069 a         2188 c         2401 g         3444 t
ORIGIN      
        1 attccgtttt catacatgtt gatgataaga taaacaaaga actcgaaacg tctggagtag
       61 tttctggaaa aggaattgtt tctgcagtat acaccataac aaaacaagac acgtcgtatc
      121 tttttgaagg attgtctacc atacttattc catcacagat tagaaattca ggaggattct
      181 tggatgttgc aaaaaaaata tctgaagaac catcttcttc tatgacattt tcaatcatac
      241 cacaaggtag ctcttcgctt tatcagcttc aggtatcaat tgacaaacca ttagaaccga
      301 atttagcaac aataagccct cttgaatttt ttgaaactga aaagcttcaa agatcaaact
      361 attttagcag cggattcttt ccactaaatt ccatagtaca agtagtagtt ttatcaaatc
      421 aaacaatgaa ggttgcagga cacgccagtg aactggtacc tattttagaa aaagacggcg
      481 aaaaataccc atcagatttt acaagaaatg ggtggctatt tgatcctgat tcaggtgatc
      541 agatagtagg aaaatatctt tttggaaaga cgaccgaagt gtcaaaaaat gatttgatat
      601 tcaatgttgg aggattaaca cagtcaactc caacaaatga tgattcgatt tacgttctta
      661 ttggtgtagg catagcagca gcgattgcaa tattttggta tcttagaaaa cctaaaacaa
      721 aatcagagaa gtaatactgc tgcagcaaac acagtggtcc aacgcccttt ggcatcaccg
      781 actgcacttt gtgtgatatt tcttgtcttg tatatctcgc ctgagatctt ccattgcttt
      841 cgcttttcat cccagctctt gttgatatca aattttatgc caagtgaaga ggcaagcatt
      901 tgtgcagcaa tgtcttcagc ataatcgcca gcttcttttt ctgtttcacc aaatgaatgg
      961 tattctgata gatagccgta cctatttttg tcagtaggtt gagcaatgcc aacagaagct
     1021 gccatcagcc tatgtggctc attgctttgc tgtcgtgcat agattacaaa ttgtacgctt
     1081 ccaggtctta tcaattttag tccttcttct cttgaaacca atttggcatt gggtggaaat
     1141 atactagata tcaatacaag atttgttcca gcaatgcctg catcacgcaa agcatattca
     1201 aaacttgtta gctgatcttc gtgtactccc ttacctctgg taaggaataa tttttttgca
     1261 actaagtcta gcacgaatcc actctaattg cagatcttta aatatttatg tttgaatcca
     1321 aaactagaaa aaatcacttc aagtttttca aaacatcaag aattttctgt ccatgagagt
     1381 ctacatctat gtccctgaaa atttttacaa cctttccttc tttgtttatt atgaacgttg
     1441 aacgttttgc aagaccagct aaattcaagc cagcataagt cttacaagcc actttttttg
     1501 tgtcgctaag atgaagatat gggattttat attcttcaac gaatttcttt tggtcttcta
     1561 cagtatctac ggatattgca aaaagtaccg ttcctgaggc aagaatctga gggtgtgcca
     1621 atatcatact ttcagccatt ttgaacactt tttttgaagg gcatgcaaaa agatggtttt
     1681 ttggataaaa gcacaaaacc acattttttt gtcctctgta ggagcttaga tttaccagac
     1741 taccatcatt tgcaggtagt tcaaagtctg acgcaatttc tccttcttgc ataatttgta
     1801 atacaacatg taacttaata ctattttgca agatgaagct ttttgtgata ttactttttt
     1861 tcctttttct ctttttcagg ttttggattc agaaaaagat atcctacaaa tccgccaagt
     1921 gccaggttga ctactcccaa cattgtaaca tatgaagaaa acaaaataaa atttccaagt
     1981 attattcccc atactccagt gcccaagaaa gcaaacatca tgactttgag ttttgttgga
     2041 ggaattttct tcatgaacgt gctctatttt tgacactatt aaagattgac agaaaataac
     2101 aagggttaaa ttggcaaata aaaaaacgcg atttgtgcgc atagtcactg tcggtagcag
     2161 agtttttgtg acaagttttc agttagcagg agtacaaggc acaattgtcg aatcacctga
     2221 aaaagcactt gtagaaatta caaaattaat gaatgattcc acaattggat tggtacttgt
     2281 aagtgatgat atttcaaaaa ccattggtaa caagttaaca gaattacgag caaaaaaatc
     2341 aacgccactt gtgtttgaat tgccttcgcc aggaagtcaa aaaggtgaag tcgattatag
     2401 attattgcta aaacagatac ttggagttta atccatatac tacgttttat agaaaaatga
     2461 catgagagct gcgtttgtga aagacccatc tcaggttgag ataagaacca tcgacaagcc
     2521 aagtataagc aatggggaca tcatggtacg aatgcaggca tgcggtgttt gcggttcaga
     2581 cttggaaaaa atctacgggc aatatagtca accatcaatg cgtcttggtc atgaacccgc
     2641 aggcatagtg tccgaagtag gaaaaaatgt tacagagttt aaaaaaggag atagagtttt
     2701 tgtgcatcat catgtaccgt gttattcctg tcacttttgt ttacatggta atgaaaccat
     2761 gtgcaaaaaa tattctgaaa caaatctttt gccatgtggc ctagcagagg aatttgtggt
     2821 tccagaatgg aatgtatcac atggcggagt aataaaaata ccagatttga tgagttttga
     2881 agaagctgct atgatagaac ctcttgcatg ctgtgtcaga tcatggaaca agatccaagt
     2941 caaacatgac gattctgcgg caatatttgg agtgggtcca acaggaatga tgcatgtaat
     3001 gttgtgcaag gtttatggat taagagatgt tttctgtctt gatgtcaatg agtttaggtt
     3061 agattttgca aaaaaatttg gaataacaga agcaataaaa tcaactgatc ctaatgcaaa
     3121 cgaaaaaatt ctatccaaaa cagaaaaccg aggagttgac atagcgattg ttgcgacagg
     3181 aaatcttaat gcaataactc aggcaatcga atttgttaga aaaggtggaa ccataatttt
     3241 gtttggagtg ccaacaaagg acgcaaaact ttctgtcgat atgagcaaag tgtactctaa
     3301 agaaataact atcataccaa gctatgcagc gtcggacaaa gacacgttaa atgcattcaa
     3361 gctaatacaa gaaaaaaaga cagatgtaaa aaaattaatc actcacaaat ttgatctttc
     3421 agattctcac aaagcattag aatacgcaca tcatggaaat gattccatga aaataatcat
     3481 aacaaattca gaaacgctta actaaggcaa actttctttt ttgaatttaa gggaaaacaa
     3541 tggactgggg aatgaaaaac cgtctggcaa gaataatcaa gccagagacc gggcgcggag
     3601 taatgttggc tgtagaccat ggatattttt taggaccaac agaaagactt gaagttccgc
     3661 gcaaaaccat tcaacctcta ttaccatatg ccgactctct catgttaaca cgtggtgtgc
     3721 tacgcacgtc agttgatgca aactttccag ttccagtagt tctcagagtg tctggcggat
     3781 caagtatcat aggaaaggat ctctcaaatg aaaaaatcac cacatcaatc aaggaagcaa
     3841 taaagcttaa cgtgactgca cttgctctct caatctttgt tggggcacag tatgaacatg
     3901 aatctcttgt tagccttggc aaacttgtag atgagggtga agaatacgga ataccagtac
     3961 tagcagtaac tgcagttgga aaagaacttg aaaagcgtga tgcaagatac ctatcccttg
     4021 cttgcagaat tgctgcagaa tttggggcgc atcttgtcaa gacatactat tgcgaaaatt
     4081 ttgaaaaagt agtaaatagc tgtccaatac cagtaatcat tgccggagga ccaaagcttg
     4141 ccaacgaact tgatgcttta caattgacct atgatgccat acaggcagga gctgttggag
     4201 tagacatggg ccgaaacatc tggcagtcag actatcctgt ttcaatgata aagtcagttc
     4261 gtgcaatagt tcatgaaaat gcaacagtca aagaagcaca tgaattgttt accaagctca
     4321 aaaatacaga acaatcggca aaaactgcaa catcagtcta gtcagaaatc ttggtcatgt
     4381 gtatatgaac aatcttccat ctaggagatc ttaccaaaac aaaagtcgcc ctgctttcca
     4441 tagagagatg ctgacctcta aaactatagt tgtctacaag aactccactt tgttttagtt
     4501 gaaaggtaac cactgcacta tctccaaaaa catcaactcg ttggtttttt atttcaaaat
     4561 cataatcaga tatactaaca aaccgcagtt cttgcaatgc aattgttgtt gcatagtctt
     4621 ttagatcaag aggtggcaca tcactgtagc ttgaaaatct aggatcatca agttgtattg
     4681 ctcttaacgg tgcaaggttt tttgttttac caacttcaaa aaaatgacca attatttcta
     4741 ggatttctga tgtttctgac aaaattgctc tttgcttgag gtggtttgct gtttaagttt
     4801 tgtgaaaaca tgcataaaat caaaagtttt agatcacatg taggtctcaa ttttgaaata
     4861 attactaaac tggctataga tttttgatga ttttagacaa tttttctaga aatatttata
     4921 aattatatcg aacgaaaaac aacagactga tatgaagtta ggaggttcga cgggcatttc
     4981 tagtattttg ttgctagtcg acctgatact gtagattttt cagttttact tttagtgaaa
     5041 taatatggaa aaaatgtcag gtgctagatc attaatggtt gcccttgaaa gggagggtgt
     5101 agagatagcc ttcgggctac ccggtggtgc aaacttgcca atttatgatg agctttacaa
     5161 aagtaatatt cgccacatat tggtaagaca tgaacagtct gcagctcata tggcagatgg
     5221 ttttggtaga gtaagcagaa ggccaggagt ttgttttgct acatcgggcc caggagctac
     5281 aaacattgta accggtattg caacagcgca agctgattct gctccaatgg ttgcaataac
     5341 tggtcaagtt cctttgaaga tgataggtag ggacgcgttt caagaaagtg acataatagg
     5401 aatttcaaat ccaattgtaa aatatgcatt ccagccaaca actccagctg aaattccaga
     5461 ggtagtaaaa aaagcatttt acatttcatc tactgggaga ccgggtccag ttcttatcga
     5521 cataccaaaa gacgtacaac aaaatgaggc agagatacca tttccagcag aaatcaaagt
     5581 aagaggatat catccatggg ttgatccaga tgtacaacaa atcgaacgtg caatagatat
     5641 gctactttct gcagaaagac caatcattct tgcaggtggc ggtgtcataa tttcaggtgc
     5701 atttgcagaa ttacaatcaa tagcagagct tctaatgatt ccagtagtca ccaccttcaa
     5761 aggcaaaggg tcttttcctg agaatcatcc attatcactt ggcccaatag gaatgcatgg
     5821 tcatgcagaa gcaaacaaaa tgatgacaga ggcagactgt gttcttgcag caggttccag
     5881 attttctgat agatcagttg gaacctttga agagtttgaa aaaagattaa agatcataca
     5941 catggatgtg gaccctgcag aaattggtaa gaaccaaact acaactgttg cagtggtggg
     6001 agacatcaaa gcatcactta gaataatggt gaagatgtta attcaaaaag caatcaaacg
     6061 aaatggagag tatccttggc taaagagagt caaagaaaca aaagaatact ataaagaaaa
     6121 cttgaagatt catgaacatc cacttgcagc ggccaaagta ttacgcaaac tacgtgaagt
     6181 tcttccacat caatcaattg ttacaacaga agtaggccag caccaaatgt gggcatcatt
     6241 attttttgat gtaatacatc caggaacttt ctttagttca acaggtcttg gcacaatggg
     6301 ttggggattt ccagctgcaa tcggtgcaaa agcagcaaaa ccaggtgttc cagtagtaga
     6361 cattgcagga gaaggtagtt ttaacatgac agaaaattct cttgcagttt cagtattgga
     6421 taatttacct gtcatcgtat ttgttatgaa taatttttca ctcggaatgg ttgcacaatg
     6481 gcaaagaaca ttttatgata gaaggatgat ggcaattgat cttaaaaatt gtccagatta
     6541 tgtcaaaata gcagaagcat atggagcaca gggaatcaga gcacagtcac ttgatgagat
     6601 aggtaatgcc gtaaagactg cactaaaaag tgatgtagcc acagtgattg acattccaat
     6661 tgacccgcag gaagatgtct taccgtttgt agctcctggc actccactaa aggacatgat
     6721 attaccttca taggtgattt taatgtggaa tataatttct gtactagtag agaacaaacc
     6781 aggcatcttg ttcaaaatta caaacttgtt tagatcaaga aactacaaca tcgagagcat
     6841 atcagtaggc ataactgaaa atcctgaatt ttcaaaaatg acaataacta caagtgctga
     6901 cgaaaaacaa gtcatacaga taataaagca acttgacaag atgattgata ccatagaggt
     6961 aaaacgtcta gatgagaaga tgagtgtgta cagagaactt gtccttatga agataaaagt
     7021 aaccaaaccg tcagacagca tggaaataat gaatcttgca aacgcctttg aatgcaaagt
     7081 tcatgatgta agaaagacat caatcatggt agagataact gcaacacctg acagaattaa
     7141 tgcgttccaa gatctagtaa gaggatatgg aattttggac attgccagaa ccggtgtatg
     7201 tgcgcttccc agaggtggat taaatgaaag ttagaatttt tgacactacg ctcagagatg
     7261 gagaacagac tccaggcata gctctagctc cagaacaaaa actaaacatt gcaaaaaaac
     7321 tcgatgcatt aggagtggat gccatagaag ctggctttcc aataatttca gaaggagaga
     7381 tgagtgcaat caaaatgata actagcgcaa acttgcgtgc cgaaatctgc ggtttagcaa
     7441 gaactgacaa aaaagatatc gacgctgcag taaattcagg attaaaatat attcatactt
     7501 ttattgcaac atcagacatt caccttcaat acaaattaaa attaacacga gaacaggcac
     7561 ttgtaaaagc aatcgaagcc gtagaatatg gaaagtcacg aggcttgcag gtggaatttt
     7621 ctgcagaaga tgcgacaaga acagacagag aattcttgaa gaaagtcttc aaggcagtag
     7681 ctgaagctgg agcagataga attgacatac ctgacacagt aggatattcc acaccagaat
     7741 acatggcaaa aataacaaag gatgctgttg aagcttccaa acttccagta agtgtccatt
     7801 gtcataatga ttttgggctt gcagtagcaa atgcaatttc tggaatacag gcaggtgcaa
     7861 aatgcgcaca cgtaacaatc aacggcatag gagaaagagc aggaaatgca tcacttgaag
     7921 aatttgtcat ggcactacag tgtctccagt ttgatcagaa atttgaaacc aacattaaca
     7981 caaaattact ctatgaaaca tcaaagtatg tttcaagtgt agttggtgtg catgttcaac
     8041 caaacaaagc aattgttgga gagaacgcat ttggccatga gtcaggaatt catactcatg
     8101 gagttttaag caatccatta acatacgagc ctatcagccc agaacttgtt ggaagaacaa
     8161 gatggtttca agttggaaaa catgcaggag ctcatggtgt ttctgccatg cttgaagaat
     8221 atgggataca accaacaaaa gaacaagttc atgaaattct agagaatgta aaaaaactcg
     8281 gtgatcaagg aaagcacgta acagatgtag agttattgtc aatagcagga gaggtgatga
     8341 aacagccagg gcttagaaga ctagttcatt taacaggatt ttcagtttca actggaattg
     8401 gcaacatgcc ctacgccttt gtcaaattga acatagaagg aaaagacttt atcggcacag
     8461 actatggagt tggcccagta gatgcatcaa taaatgcaat acaaaaaatc acaggagaga
     8521 ttgccaaagt aagaatcaag gactataggt tggattctat tgctggcggc tctgatgcat
     8581 tatgtgaagt cacaataaaa gtcgaagacg catatggaaa tcttgcttcc gcaaaatcaa
     8641 ttggagaaga cattgtaatt acaagtgtac aagcaatgat tgatggaata aacagaataa
     8701 tgctcaaaaa tatattaaaa caaaaaaatc tgggaaatta aatgtataaa atttctctca
     8761 taacaggaga cggcataggg cctgagctat cagaatctgt tgtcagtgta ttagatacga
     8821 taaatgacaa gcttggaata aaattacaaa ttgaaaagct tgaagctggt gacaaggcat
     8881 tagcaaaaac tgggaaggca ttaccagagt ctacattaga gtccatcaga aaatcagatg
     8941 cgtgtttgaa ggcgccagta ggagaatgtg cagcagatgt cattgtggtt ttaagaagaa
     9001 tactaaatct ctatgcaaac atcagacctg ccaagtcgta tccaaatatg cctgcactaa
     9061 aagataacat tgatctggtc atagttaggg aaaacactga agatctttac acaggaatgg
     9121 agtttgaaat tggaaatact gccattgcct tgagaaccat ctccgaagag gcatcaaaaa
     9181 gaattgcaaa atatgcgttt ttgactgcta ctcaaagaaa ccagaaaaac aaggtcacat
     9241 gtgtacacaa atcaaacgtt atgagaaaga cagacggtct ttttgcaagg gcgtgtaatc
     9301 aagtggcaaa agaatttcca aaaatccagt tcgagcagat gtatatagat gcctgtgcaa
     9361 tgaatctcat aaggcagcca gaggcttttg atgtcatagt tacgaccaat ttgtttggcg
     9421 atatattatc agatgaagcg gctcaggtag ttggaggact aggaatggca cctgctgcaa
     9481 acatcggaga tgattatgca ctatttgagc cagtacatgg ggcagcattc gatattgcag
     9541 gaaaacaagc tgcaaatcca tcatcgttta tactttctgc caagatgatg ttagaatggc
     9601 ttgcaacaaa aaacaatgac cagaaatgtt ttgatgttgc aaaaaaaata gagtctgccg
     9661 tatatggagt tgtaaaagaa ggagctaaga caaaagacat cggaggtaac aaaacaactc
     9721 tagaatttac acagcatgtg atttcaaaga tgatctaaat gtcagaagaa ggtggatttc
     9781 tagaccaaaa aaacatcata aaccccgcag ggatcatgaa caagagtgct gcgataaatg
     9841 ttatccaata aaacgttgga tccaatcctg gaatcaacgg gattggaaat actgcatatg
     9901 cccatacaaa tacaacaaat ccaagcaaaa ctttgtttcg tttaaaaatt ttccatccaa
     9961 gttttctttt atcaatgtag catcgcttgc atctacttct atcatctgtc atgcaggttg
    10021 tacaacaatg tctttcacaa aagtaacact tgcgttctcc aaactgcgag ccacagaaat
    10081 cacactgttg cactgttact ttatggcaaa ttttggattt atctttttat ggaaagagtc
    10141 gctccggatc gcgcggatac aagactactt ctctcacatt atcaatctgg gtcaaaacag
    10201 acatcagcct atcaagacca agtccccatc cagaatgcgg aggcattccc cagtcaaatg
    10261 ctttcagatg gtcttcgaat tgatgcggat caagaccttg ctcttgcagt ctcgttttta
    10321 gttttatagg atcatgaaga cgcgtaccac ctgatgagag ctcaagataa ccatactgaa
    10381 gatcaaatga gcgtgataat tctggatcat catctttttc gtgtatgtaa aagggtttta
    10441 gcttcattgg ccagtcaatt aaaaaataaa atcctggatg tcgctctcca agcttacgaa
    10501 gatgcgcatc ctgcagatca tctccaacct caagttttat tccatcacga cctagctctt
    10561 caattgcttt tttgtatgat attctttcaa atggaacaga aggttttttg atctcaatgc
    10621 caagagattt ttgctcagaa gcacaatttt gcatcacata ttcaaacaca tttactacta
    10681 cagattccaa tatatccata acatcagagt aatccatgaa tgcggattca atatcaacac
    10741 tggtaaattc tgtcaagtgt cttaccgtat gagatttttc agctctatag tatgatgcaa
    10801 tttcaaaaat tctttcaagg ccaattgtta attgctcttt gtatagctgt gggctttgtg
    10861 caagatacgc ctgcttttca aaatatttta acgaaaaaag gtttgcacca ccttcacttg
    10921 cactgccaat taattttgga gtgttgactt caatgaaact tttttcggtg agagtttttc
    10981 gaattgaggc aagtacatgg tgtcgcattt taaatattgc agcagttttt tgattgcgta
    11041 agtcaagtgc tcttgcattc aatctattgt caatgttgct ttccaatcta ccaataggat
    11101 ctattggaag tggttgtacg gctactgtaa gtatgtcaac tctttcagat tttacttcaa
    11161 atggaaaatc tctagctttt gatttttgta ttgtaccggt tacttttatg acgctttgtc
    11221 tggtaaggcc cttaatctgt ttgagagagt cttctccaac tagaactatt tgtgcaaccc
    11281 ctgtcacgtc acgaagtgtt agaaaggtca gtttaccaag ttcgcgtacg tcttccaccc
    11341 atcctccaac tataactttg gttccttcca tagaaggact gagatttccg atgtaatgtg
    11401 ttcgtttaaa atccataaat tcacactagt gtttaatctc aaattctgcg tcaggtttga
    11461 ttctacgtag attcattgct acagaaatta tttttgcgtc aaagctacaa attgaggtcc
    11521 tcaaggtaaa tcggtttttt aacttactaa aactaggata taaccccttc ttccaaaacc
    11581 cacatttagt tcaatgatcc aaaatgatcg atgactttca tttctgttga aaatcttaag
    11641 acgcactatg aaacagagaa tggaactgta catgctgtcg acgatgttag tttttctctg
    11701 gagaagggag aatctgttgg gattgcaggg gagagcgctt gtggaaaaag tactcttgga
    11761 ttatccatta tgagaatatt gcaaggcgga aaaatagtat cagggaatat tttattagaa
    11821 gacaaatcaa tattgagaat gtctgattct gaattttcaa aaaaacacag atggaaagac
    11881 atttcgatgg tttttcaagg cgcaatgaat tcgttagatc ctgtattcac aataaagcag
    11941 cagtttatcg aggttctcaa acagcatcat tacgaaggaa attatgatga cacaatctcc
    12001 gaaatggtaa attctgtagg tctcgatgtc tccgttctca aaaaataccc tcatgaactc
    12061 agcggtggaa tgaaacagag aatagttatt gcaatggcac tt
//
LOCUS       DATIDL010000085        15379 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrosopumilaceae archaeon isolate SMAG_U16342
            k141_2526574, whole genome shotgun sequence.
ACCESSION   DATIDL010000085 DATIDL010000000
VERSION     DATIDL010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935836
            Sequence Read Archive: SRR25158326
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosopumilaceae archaeon (soil metagenome)
  ORGANISM  Nitrosopumilaceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrosopumilales;
            Nitrosopumilaceae.
REFERENCE   1  (bases 1 to 15379)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15379)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/04/2024 05:09:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,520
            CDSs (total)                 :: 1,474
            Genes (coding)               :: 1,474
            CDSs (with protein)          :: 1,474
            Genes (RNA)                  :: 46
            rRNAs                        :: 1 (5S)
            complete rRNAs               :: 1 (5S)
            tRNAs                        :: 43
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15379
                     /organism="Nitrosopumilaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2526574"
                     /isolate="SMAG_U16342"
                     /isolation_source="soil"
                     /db_xref="taxon:2202732"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            255..455
                     /locus_tag="VLB45_03745"
     CDS             255..455
                     /locus_tag="VLB45_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008297448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04849.1"
                     /translation="MNKREKDPYRKLTVEGEKKTQWICGCFETADGFFKFCNEHSETI
                     RKTIEAQIDELDMTMVVDEKKS"
     gene            complement(452..2494)
                     /locus_tag="VLB45_03750"
     CDS             complement(452..2494)
                     /locus_tag="VLB45_03750"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HSD04850.1"
                     /translation="MALQDLIIPVGAAIVSFAIAAIFAAWVSKQKPGTQQMLEISNAV
                     KVGAMAFLRREFKIVIPVAIGLSVIIGAAIGFSNGIAFAVGAGLSAVAGLISLKITVK
                     AAVRAANASGQGLGHTFALAFRGGATVGLAVPAMALLAITLLYFVFPDPITIAGVGIG
                     ASLIALFIRVGGGIFTKAADMGADLVGKVEANIPEDDPRNPATIADNVGDNVGDAAGM
                     GSDIYESYIVTMLASLLLAALIGTPENLLYPILIGASGLIASIIGTATIGPRKMTDVM
                     KPLNRAFYVSAAIAIGLNFVFTFMFLGQTPLAYALFGTTVVGVILVPVIQRITDYFTS
                     YKFKPVQEISASAKWGYASLTLMGIIQGMRSTGPFMIAIVVALALSFAIASSAAPDPS
                     QATLYGIFGTSLTAMAMLSLAGIVLSIDAFGPISDNAGGIVEMTGMGESNRKVTDEID
                     AVGNTTKAVTKGFAIASAGLAALAMIQAFQYEAGKIFHVAFDYSLSNPSIIIGLLVGG
                     LLPFFITSQLIAAVSRAAAKMVEEVRRQFKEHPDILTGKTKPEYAKCVDVATVASLRE
                     LWKSATLTIVAPIILGVILGPPAVAGLLMGAVVTGIFLAYHLANTGGAWDNAKKYVEI
                     QGHKGSEEHKITIVGDVIGDPYKDTAGPALNTVIKLLNTVAIVFVSAFVGLLVLVR"
     gene            complement(2547..2909)
                     /locus_tag="VLB45_03755"
     CDS             complement(2547..2909)
                     /locus_tag="VLB45_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010191216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04851.1"
                     /translation="MKLDSDLRFKILEKTGIYANRFGISEPKVLLTTKEVLEMPKEIT
                     EGRRTSAYKYFGVSYMQHNVVFLNVRKLPDEKALENTIVHELIHLRFPYLSHGKRFNK
                     LVRQGLSGKTFAPYKKRK"
     gene            complement(3067..3564)
                     /locus_tag="VLB45_03760"
     CDS             complement(3067..3564)
                     /locus_tag="VLB45_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010191220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-dependent dephospho-CoA kinase family
                     protein"
                     /protein_id="HSD04852.1"
                     /translation="MQLPENLRDHLKKPLGILLKDSDTSKENILKNIPSGSFIISVGD
                     ATTDKLIKYDIIPSLQIVDGLEKRSKRDLPASSEKTIFSCTCINPAAQITIESINAIK
                     KALQNPPARIIVTGEEDLLVLPACTLAPQNSVVLYGQPNEGLVIVQINSEIRNKVQAI
                     MDAMS"
     gene            complement(3585..4673)
                     /locus_tag="VLB45_03765"
     CDS             complement(3585..4673)
                     /locus_tag="VLB45_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140663 - ATP-dependent FeS chaperone
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HSD04853.1"
                     /translation="MVAVDEVLKTLSTVIDPDLKKDIVSMGMIKDLEINGNNLKFTLE
                     LTTPACPFNDQIEQDVRNAIKGIPGIGNLDMKISAKVMEGRALSMDEMMPTVKNIIGV
                     ASGKGGVGKTTVSVNLALAMAKSGAKVGLLDADIYGPSVPLMLGMGKSTMEVENNKLQ
                     PAESHGLKVVSFGFFADQEHQAAIYRGPIISGIVKQFLVDTNWTDLDYLIVDLPPGTG
                     DIPLTLAQTIPITGILVVTTPQEVASNVAVKAFGMFQKLNVPILGVIENMSYFKCPNC
                     DTNHHIFGKGGAKKICEKYNLQFLGEIPLNPGIMEGSDTGKPVLVTDPNSPHGAAFMS
                     AAKNVAARCSILAAQLKEEMSAEVATQK"
     gene            4770..5363
                     /locus_tag="VLB45_03770"
     CDS             4770..5363
                     /locus_tag="VLB45_03770"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase"
                     /protein_id="HSD04854.1"
                     /translation="MFSISTLTDVVRIPPKLFGDSLKKAAITILREKYESMINPELGY
                     VIMILDVKVEDMGKIVSGDGGTFHKVEFNALTFYPKLQEIVRGEIVEITDFGAFVRIG
                     ATDALLHLSQVMDDYLKSDVKSGLILANQSGRTLKIGSTIRARITAVSLGKSAAMKIG
                     ITCRQPFLGADEWIAEEIKKAKAPAVQAEKAKKAESK"
     gene            5371..5559
                     /gene="spt4"
                     /locus_tag="VLB45_03775"
     CDS             5371..5559
                     /gene="spt4"
                     /locus_tag="VLB45_03775"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003711 - transcription elongation factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor subunit Spt4"
                     /protein_id="HSD04855.1"
                     /translation="MVKEMACRKCKCVTIGKICPVCKSSDLTPDWRGSVLVVDPATSV
                     VAKSLGITEKGKYALKVT"
     gene            5561..6388
                     /gene="nadC"
                     /locus_tag="VLB45_03780"
     CDS             5561..6388
                     /gene="nadC"
                     /locus_tag="VLB45_03780"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HSD04856.1"
                     /translation="MHQSAKKALASFLAEDIGKGDITSNLLPKKIISASIISRQRGMI
                     AGVQYAKEIFAIKRCRVKILKKDGSQVKPNEKVMIISGAAPSVLSAERTALNLLSRMS
                     GIATQTNTLVQKIRKVNPKVKLYSTRKTAPGLRFFDKEAVAIGGGHKHRMSLDQMIMI
                     KDNHLAVSGSVYPLLDKARKKHKKIEVEVENLHNAIVSAQGGASIIMLDNMNPKEISQ
                     VVSTLKKLGLRNRVKLEASGGIDSTNIQSYAKTGIDMISVGKITNSVSGLDLSLEVN"
     gene            complement(6381..7319)
                     /gene="nadA"
                     /locus_tag="VLB45_03785"
     CDS             complement(6381..7319)
                     /gene="nadA"
                     /locus_tag="VLB45_03785"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HSD04857.1"
                     /translation="MNLKSEILKLKKEKDVLILAHNYQIPEVQDVADYVGDSLGLSRQ
                     AAKTNQKTILFCGVHFMAETAAITCPDKKVLIPDLSAGCSLADTITVDELKNWKAQHP
                     NAIAVGYVNTSAEVKSELDYCCTSSNAVNVVKSIPENKEILFLPDMFLGSYVAKMTNR
                     KNMFIWAGECHVHAGIRSQDVQEKLNQYARSEFLVHPECGCTTSLMYDVSQGDYGSRQ
                     VQLLSTEGMMNYAKSSQAKSFVVATETGILHRMRQQNPEKDFIPVSENAVCQYMKMIT
                     LDKVYASLKEDKYEVKVSEKVAQKARIAIDRMLAIN"
     gene            complement(7416..8234)
                     /locus_tag="VLB45_03790"
     CDS             complement(7416..8234)
                     /locus_tag="VLB45_03790"
                     /EC_number="1.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate dehydrogenase"
                     /protein_id="HSD04858.1"
                     /translation="MKKIGLLGCGAIGTEIALAIDSGKIPATLTHVFDFSKSASEKMV
                     EKLKNKPTITENVGLLAASPVDLIVEAASQDAIRDNALSILQNRKDLMIMSVGALLDE
                     SILDIIVEGCNDFKKSVYLPSGAIVGLDGIRAVKDELESVTLVTTKSPRSLKGAKFFE
                     TSDIALDKIEKPTVIFDGIAQDAVRLFPANINVAALLSLAGLGSEKTKVRIVADPKTD
                     KNTHEIIAEGKFGRFSIKVENVPSATNPKTSKLAILSAIECLNAICKQEIRVGT"
     gene            8318..9193
                     /locus_tag="VLB45_03795"
     CDS             8318..9193
                     /locus_tag="VLB45_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HSD04859.1"
                     /translation="MNSNKSGLMEKFLFGIAKQWIAGDSMEQAIESARLANKKGMDAI
                     INKLGEHLASKTQVENTVKEYIMLVSNLRKSKVEGGISIKPTQLGLSRSKKECLQNYS
                     IVIEKAALSQAFVWIDMESSEYTDDTIGIYLDLFDKYERLGIAIQANLRRSRGDLMRL
                     VEKGAKIRLVKGAYRENKQIAFQSKSEVDQNYRNLMEMLFVKANEFGIATHDSVLIEK
                     AIKLSKAHEKKFEFQMLKGIRDELKPSLVKKGFSISEYIPYGTNWLPYSIRRLKERKR
                     NILLLGSSFIQSHRV"
     gene            complement(9175..10740)
                     /locus_tag="VLB45_03800"
     CDS             complement(9175..10740)
                     /locus_tag="VLB45_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010191232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HSD04860.1"
                     /translation="MFENEKTWSRAVSNNTTDEFHKKFDKAVESIKNDFGKSYPMIIG
                     GKEIHSENQFQVRSPADRNIILANFPLATKEDTIHAIESAKDAFHRWRDTPYQKRVQI
                     FREVADDFSSQKFTLAAIMTFENGKSRLESMGDIDEAIDFLRFYAELLETNQGYSKET
                     KSANPNEKTHSVLKPYGVWGIISPFNYPSAIAIGMTTGALLTGNTVVLKPASDTPLSA
                     YKFVNAVYHKLPPAALNLVTGAGSVVGKTILESNLVDGIAFTGSRDVGVSGFRTFIEQ
                     NPRPFISEMGGKNPVIVTESADLDKAAEGVMRAAFGYGGQKCSACSRVYVQKNIASQF
                     MEKFVAKTKSLKIGKPWQKDVFLGPIINESALKKFEKVSEIAKKDGKIVHGGSVLKDQ
                     EYKDGYFAEPTIVTGLPQEHELLRDELFLPFVCVSEFENFEEAIKLANKTDYGLTAGI
                     FSQDKNQINTFFDKIEAGVTYANRAASATTGALVRAQPFVGWKDSGISGKGAGGEYYL
                     LQFMREQTQTLCD"
     gene            10842..11681
                     /locus_tag="VLB45_03805"
     CDS             10842..11681
                     /locus_tag="VLB45_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010190781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA double-strand break repair nuclease NurA"
                     /protein_id="HSD04861.1"
                     /translation="MRNTVYLDALKKREETLSILKGQRFEQILQEAKNNWVEYIPQRQ
                     DSVIAGIDSSYNSTKFQGLELWVVAAVSIKSDGTMISELHNQGLGTPNPELAAQASKM
                     EIDACTASVDEADVVALDGSLYSQFLTRQASLIAMLTSAIKKKNNVIFVSKTSVTKNE
                     FGHLGSLAGDIFYYNHAVKKPGFSKMFVDKDLGPGKVISSVYARLRDSTPLIKIELFG
                     SDHDESEIKSLLDRLYKNSVSGYPYALKLAHNNCKISYSDLARLVSLYGLGNETGSRE
                     VLE"
     gene            11678..13198
                     /locus_tag="VLB45_03810"
     CDS             11678..13198
                     /locus_tag="VLB45_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSD04862.1"
                     /translation="MNIGVVIGESKPTEITAQSVRPLSVGEYVIVSSQNGKLLGLVER
                     SIVSSDALTDVRNFDEAMESKEVAELNSRDKNYKVKIGILGFLDNLQKGQAIVPAVPP
                     LPGTSILEATQEDLGIIFAPKEKEWIRIGSLLRNSEIEAKINLNKIVSRHIGILAMTG
                     MGKSNLVSLIAKQVADLQGTLIIFDYHNDYTNLSLPKISVADAKINPRLLQADKLAEV
                     IELRENADKQQRVLRESFTASVRQAKDFWTALEDSVSEFGHTTKGYADSASRVLDKID
                     DAKHRFSDILDPDCGDPVDLIKEGRVNVLSVAELSERQANVGLSYYLQELLNDRKDSY
                     WAKKGRKLSRKNTKFASPLFVIIEEAHVFIPKGEHTDTKYWASKVAREGRKFGIGLGI
                     VSQRPRNIDPNVLSQMGSLAIMKIVQDDDQQQIASAAESLSRDLLTQLSSLNIGDAVL
                     VGQWVNLPSIVHVDEVKGKSIGSDLNAVAEWSESTKFKDIAAESTQGLIQKDLLMD"
     gene            13200..14342
                     /locus_tag="VLB45_03815"
     CDS             13200..14342
                     /locus_tag="VLB45_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HSD04863.1"
                     /translation="MIFSHISDTHLGFVQYYSEEREQDVYTAFDQAIDTSINDHVDFV
                     ILAGDIFHVPNPSGKAILVLANALKRLKQKNIDSFFILGEHDISRIRATPIPFVYHNL
                     EFSKYIGNGKPLYYKDVMLVGFDKRRKSEIPTFENDFAKADSEAKKHTGHKILVLHQG
                     IGEINKFAGELNSTDLPKNFTYYAMGHLHERELKKFNHLGGPLAYPGSIELTSSEGIK
                     ETQKGFYQVDLTSSEAKPNWIKLDTRPQLSVKTDFENLKKEVDMLAEKIQSFSRKPVV
                     EIKIHGENIETDLVQSQIARLQPLALRCFWKAIREEENSGLTFVDRPAKIDDEMFKLA
                     KDILNSEPWANFTIRDLLPLLSTNQIDEANHLVMENFVRFKKEKKC"
     gene            14336..>15379
                     /locus_tag="VLB45_03820"
     CDS             14336..>15379
                     /locus_tag="VLB45_03820"
                     /inference="COORDINATES: protein motif:HMM:NF024573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HSD04864.1"
                     /translation="MLTEIEMGNFLSHSNTKLPFDKGVTVFVGHNGAGKSSVIDAVTF
                     ALFGQHTRNSNKGLVRRGSNQAYAKVDFTISNRAFEAVRKIDSKGTLSAQLFEKKDGS
                     LVPLAAGERKQFGESMTKEIESLIGMDFDKLKIASIVRQGELNSIIDADPKKFKELVN
                     TIIGIDKLDTAFGLMKDVVENFRSSIRKDLEYDDTDIESLRSNLDVITKEIQVAEPQK
                     EELTIKKQKQEHEITLLQKKVDAESPKESKIKELDTRKSELVRYVKTAIASLQKDVLE
                     KERKLSDCEGCFDHLFSKKEIEAQMEKIEANLESTRNEIRQLSEKVGNLGGQEELAAK
                     LKLKDGKCPVCDSV"
BASE COUNT         4862 a         2972 c         2970 g         4575 t
ORIGIN      
        1 tgcgacatag aatgttaaac aataagatgc ttatgaaaat tcactagatt ttcatttttg
       61 ataatgatta attttattga tcttatatgt aagtaatgga attaaaatta taacaatatt
      121 attcctcttc attgtgggtt caaacttatt ttacgtacaa agtgggccca aaaataagac
      181 catgatggta ccctagtccc atcggactcg catcttggaa atctttttag aatgatctgt
      241 aatgatgtgg tgtaatgaac aagagagaaa aagaccctta tcgaaaacta actgtggaag
      301 gggaaaaaaa gacacagtgg atttgtggct gctttgaaac agctgatggg tttttcaaat
      361 tctgtaatga acacagtgaa accataagaa aaacaattga agcgcagata gatgaacttg
      421 atatgactat ggtagtcgac gaaaaaaaat cttagcgtac tagaacaagt agtccaacaa
      481 acgctgaaac aaacactatt gctacagtgt ttaataattt tatgactgtg ttcagtgctg
      541 gacctgcagt gtctttgtat ggatctccta taacgtcacc aacaattgtt attttgtgtt
      601 cttcgctacc tttgtgtcct tgaatctcga catatttttt ggcattgtcc catgcgccgc
      661 ctgtatttgc caagtgatat gccaagaaaa ttcctgtaac cactgcaccc atcaagagtc
      721 ctgctactgc tggtgggcct aaaattactc caagtattat tggtgcaaca atagtcaaag
      781 ttgcagattt ccaaagctca cggagcgaag ctactgtagc aacatcgaca cactttgcat
      841 attctggttt tgtttttcca gtaagaatgt ctggatgttc tttgaattgt cttcttactt
      901 cttctaccat ctttgctgct gctcttgaaa ctgcagcaat taattgactt gtaataaaga
      961 atggtaagag acctcctact aacaatccga ttattattga tggatttgaa agactgtaat
     1021 caaaagcaac atggaaaatt tttccagctt catactggaa agcttgaatc attgcaagag
     1081 ctgcaagacc tgcactagca atagcaaaac cctttgtgac tgcctttgta gtatttccaa
     1141 cggcatcaat ctcatcagtg accttgcgat ttgattctcc catgcctgtc atctctacaa
     1201 ttccgccagc attgtcactg atagggccaa acgcatctat gctgagtact atacctgcaa
     1261 ggcttagcat ggccattgca gtaagtgatg ttccaaaaat tccatacaat gtggcttgtg
     1321 atggatctgg tgcagccgat gaagcaattg caaatgataa tgccaaagct actactatag
     1381 caatcatgaa tggtccagtt gaacgcattc cttgaattat tcccatcagt gtaagtgacg
     1441 catatcccca ttttgctgac gcagaaattt cttgtactgg tttgaatttg taacttgtaa
     1501 aataatctgt aatcctttgt attactggaa caagaatcac accaacaacg gttgtgccaa
     1561 acaatgcata tgcaagtggt gtctgaccta agaacatgaa agtgaaaacg aaattaagtc
     1621 ctattgctat tgcagcagaa acatagaatg ccctgttcag aggtttcata acatctgtca
     1681 tctttcttgg tcctattgtt gcagttccaa tgattgacgc aatcaatcct gatgcgccga
     1741 ttaatattgg ataaagcaag ttttctggag ttccaattaa agctgcaagt aacaatgacg
     1801 caagcatggt tacaatatat gactcataaa tatcagaacc cattccggca gcatctccta
     1861 cattgtctcc tacattgtct gcaattgttg ctggatttct tggatcatct tctgggatat
     1921 ttgcttctac cttgccaacc aaatctgctc ccatgtctgc agcctttgtg aaaattccac
     1981 ccccaactct gataaacaat gcaataagac ttgcaccgat tccaacacct gctattgtga
     2041 taggatctgg aaatacaaaa tagagtaatg tgatagccaa taatgccatg gcaggaactg
     2101 caagaccgac agttgctcct cccctaaaag caagagcaaa cgtgtgacct agtccttgac
     2161 ctgaggcatt tgctgctcga actgctgcct ttactgtaat tttaagtgaa atcaatcctg
     2221 caactgcaga aagacctgcg ccaactgcaa aagcaattcc gtttgaaaat ccaattgccg
     2281 caccaatgat gactgaaaga cctatggcaa ctgggatgac aattttgaat tctcttcgta
     2341 aaaatgccat tgctccaacc tttactgcat ttgagatctc aagcatttgc tgcgttccag
     2401 gtttttgttt agaaacccac gccgcaaaaa ttgccgcaat tgcaaaggaa acaattgctg
     2461 cgcctacagg tataatcaaa tcttgtagcg ccatttgcta attttgccta tttgcggtaa
     2521 atataaacca tgtctgaaag atttttctat tttcttttct tgtatggggc aaatgttttt
     2581 ccagaaagcc cttgtctgac gagcttgtta aacctcttac catgacttag atacggaaat
     2641 cttagatgta tgagctcgtg aactatggtg ttttccaaag ctttttcgtc tggtaacttt
     2701 ctgacattca aaaaaaccac attgtgttgc atgtaagaga ctccaaaata cttgtatgct
     2761 gaagttctgc gtccttctgt gatctctttt ggcatttcaa gaacctcttt tgtagtcaaa
     2821 agcactttgg gttctgatat tccaaacctg tttgcataaa ttcctgtctt ttctaatatc
     2881 ttgaatctta gatctgaatc aagcttcatc cattctcatt tttgtaaatc tggtttaatt
     2941 acgactgtca attgttgtat ctgaacttgt ttactttagc tattttacca caaaaatagt
     3001 tgattagggt cagaacttgt gtagcttaat catgaatcat acagccaccg tgtcatcata
     3061 ccccaactaa ctcatagcat ccataattgc ttgtacttta tttcttatct ccgaatttat
     3121 ttgcacaatt accagtcctt cattgggctg gccgtacaag acaacagaat tttgtggcgc
     3181 aagtgtacat gctggcaaaa ctaacagatc ttcttctcca gtaactatga ttcttgctgg
     3241 aggattctgt agagccttct ttatggcatt aatgctttca atggttatct gtgcagctgg
     3301 attaatacag gtacaagaaa aaattgtttt ttcactagat gctggaagat ctctctttga
     3361 tcttttttcc aacccatcta cgatttgtag cgaaggaatg atgtcgtatt tgatcagttt
     3421 atctgtcgtt gcatctccaa cagaaattat aaacgaacct gatggaatgt tttttaaaat
     3481 attttctttt gatgtatcag aatctttaag taaaatacct aatggttttt ttaaatggtc
     3541 tcgaagattt tcgggaagct gcacaatcta ttctttgttt ttagctattt ttgcgtagct
     3601 acttctgcac tcatctcttc tttgagctga gccgcaagta tactgcatct tgctgctacg
     3661 ttttttgctg cagacataaa ggccgcgcca tgtggagagt ttggatctgt tactagtact
     3721 ggctttccgg tatcagaacc ttccatgatt ccgggattga gtggaatctc accaaggaac
     3781 tgcaaattgt atttctcaca aatctttttt gcaccgcctt tgccaaatat gtgatgattt
     3841 gtgtcacaat ttggacactt gaagtaactc atgttttcaa ttacaccaag aattggcacg
     3901 tttagttttt gaaacattcc aaatgccttt actgcaacgt tgcttgctac ttcctgcggt
     3961 gttgtaacta ccagtattcc agtaattgga atagtttgag ctagtgtaag tggaatatct
     4021 ccagttcctg gaggtaaatc gactatcaaa taatctaaat ctgtccagtt tgtatcaaca
     4081 agaaattgtt ttacaattcc agagatgatt ggtcctcgat aaattgctgc ttgatgctct
     4141 tgatctgcaa agaatccaaa tgaaactact ttgagtccat gtgattctgc tggttgcagc
     4201 ttgttgtttt caacttccat tgtagatttt cccatgccaa gcatcaaagg tacgcttgga
     4261 ccgtaaatgt cggcatcaag tagccctact ttggctcctg actttgccat ggccaatgcc
     4321 aagtttactg aaactgtggt cttgcccact ccaccttttc cacttgcaac cccaatgatg
     4381 ttttttacag ttggcatcat ctcgtccatg cttaatgcgc gtccttccat aactttggcg
     4441 ctgattttca tgtccaagtt gccaattcct ggaattcctt ttattgcatt tctaacatct
     4501 tgctctattt gatcattgaa agggcaggct ggtgtagtaa gctctaaagt aaattttagg
     4561 ttgttcccat tgatttcaag atctttaatc attcccatgg acacaatgtc ttttttgagg
     4621 tctggatcta tcacggtgct tagagtcttt agaacctcat ctactgctac catatgaaaa
     4681 aaatccctaa tagattattt aaacataggc aaaaatggaa taatcttatc caaagattca
     4741 taaattgaaa ttttatcaat tttctttaga tgttttctat atctaccctt actgatgtag
     4801 ttagaattcc accaaaactg tttggcgatt ctctaaaaaa ggcggcaatc actattctta
     4861 gagaaaaata tgagagcatg ataaatccag aacttggtta tgtgattatg atacttgatg
     4921 tcaaggtgga agatatgggc aagatagttt ctggtgatgg tggaactttc cacaaagtag
     4981 agtttaatgc attaacattt tatccaaaac tccaagaaat tgttcgcgga gaaattgttg
     5041 aaattactga ctttggcgca tttgtaagaa ttggcgcaac cgatgcgttg ctgcatcttt
     5101 ctcaagtcat ggatgattat ctgaaaagcg atgtcaaatc aggtctaatc ttggcaaacc
     5161 aaagtggaag aacactaaag attggttcta ctattcgtgc aagaataact gctgtctctc
     5221 ttggaaaatc tgcagcaatg aaaattggta ttacatgcag acaaccattt cttggtgcag
     5281 atgaatggat tgcagaggaa ataaagaaag ccaaagcacc tgcagtacaa gctgaaaaag
     5341 caaagaaagc agaatcaaag tgaaaataat atggtaaaag agatggcatg cagaaaatgt
     5401 aagtgtgtta ctattggcaa aatctgtcct gtttgcaaat catcagatct gactccggat
     5461 tggcgaggat ctgttcttgt cgtggatcct gcaacgtcag ttgttgcaaa atctttaggg
     5521 ataacagaaa agggcaagta cgcactcaag gtaacctaga ttgcatcaga gtgcaaaaaa
     5581 agcactagcg agttttttgg ctgaagatat cggcaaaggc gacattacaa gcaatctttt
     5641 gccaaaaaaa ataatctcag ctagtataat ttcaagacaa aggggaatga ttgccggcgt
     5701 gcaatatgca aaagaaatct ttgccataaa gagatgccgc gttaaaattc taaaaaaaga
     5761 tggctctcaa gtaaagccca atgagaaagt gatgataatt tcaggagcag caccgtctgt
     5821 actttctgcc gaaagaactg cactaaattt gctatctagg atgagcggta ttgctacaca
     5881 gaccaacacc ttggtacaaa agatcagaaa ggtaaatccg aaagtcaagt tgtactctac
     5941 aagaaaaact gcgccgggac tgcgattttt tgacaaggaa gcagtagcga ttggcggtgg
     6001 acacaaacac agaatgtcac ttgatcaaat gattatgata aaagacaacc atcttgcagt
     6061 atctggctct gtgtatccat tattggataa ggctcgaaaa aaacacaaaa aaatcgaagt
     6121 tgaagttgaa aatcttcata acgcaattgt ttctgcgcaa ggtggagcat caataattat
     6181 gcttgataac atgaatccaa aagaaatttc tcaagttgtt agcactttga aaaaactagg
     6241 attaagaaat agagtaaaac ttgaagcttc aggcggaatt gattctacaa acatccagtc
     6301 ttatgcaaaa actggaattg acatgatatc tgttggaaag attacaaatt cagtttctgg
     6361 ccttgacttg agtctagaag ttaattgatt gcaagcatac gatctattgc aattcttgcc
     6421 ttttgagcca ctttttctga aaccttgact tcgtacttgt cttcttttaa tgatgcgtat
     6481 actttgtcaa gtgttatcat cttcatgtac tgacatactg cgttttcaga aactggaatg
     6541 aaatcttttt ctggattttg ctgtctcatt ctgtgtaaaa ttcctgtctc tgttgcaaca
     6601 acaaagctct ttgcctgtga actttttgca taattcatca ttccttccgt agatagcaac
     6661 tgtacttgac ggcttccata atcgccttgt gaaacgtcat acattagcga tgttgtacaa
     6721 ccacactctg ggtgtacaag aaattctgat cgcgcatatt ggtttagctt ctcttgtacg
     6781 tcctgtgatc ttatgcctgc atgaacatgg cattcgcctg cccagatgaa catgttcttt
     6841 ctgtttgtca tttttgctac ataagaaccc aaaaacatgt caggcaaaaa tagaatttct
     6901 ttattttctg gaattgactt tactacattt actgcatttg atgaagtgca gcaataatca
     6961 agctccgatt ttacctccgc acttgtgttc acataaccaa cagcaattgc atttggatgc
     7021 tgtgctttcc agtttttcag ctcatctacc gttatggtat ctgcaagtga gcagcctgct
     7081 gacaaatctg ggatgagtac ttttttgtca ggacaagtaa ttgcagctgt ctccgccata
     7141 aagtggactc cacagaataa aattgtcttt tggtttgtct ttgcagcttg ccttgataat
     7201 cctaacgagt cgccaacata gtcggcaaca tcctgtactt caggaatttg atagttgtga
     7261 gccaaaatga gaacgtcttt ctctttttta agcttcagaa tttctgactt taaattcatc
     7321 tatacaaaat aaaatcacta ctcttatgca tttaaagcat aagatgatgg gcagatttta
     7381 cgagacaata ggatgccttg gcaatatttg ccatttcaag ttccaactct aatctcttgt
     7441 ttgcatattg cattaaggca ctctattgca gacaaaatgg caagcttgct tgtttttgga
     7501 tttgttgcac ttggaacatt ttcaacttta attgaaaatc taccaaactt tccctctgca
     7561 attatttcat gagtgttctt gtcagttttt ggatctgcaa ctattctgac ctttgttttc
     7621 tcactaccaa gtcctgccaa gcttaacaat gcagctacat tgatgttggc aggaaagagc
     7681 ctcaccgcat cttgggcaat tccatcaaaa atcacggttg gtttttcaat tttatcaaga
     7741 gcgatatctg aagtttcaaa aaacttggcg cctttcagag atcttggact ctttgttgtc
     7801 actagcgtaa ctgattccaa ctcatctttt actgccctaa ttccatcaag accaacgatt
     7861 gcaccagaag gaagatagac tgatttttta aaatcattac atccttctac tataatgtcc
     7921 aaaattgatt catcaaggag cgctccgaca ctcataatca tcaaatcctt tctgttttga
     7981 agtatagaaa gcgcattgtc acgtattgca tcttgggatg cagcttcaac tatcaaatct
     8041 acaggtgaag ctgcaagcaa tccaacattt tcagttattg ttggtttgtt tttcagcttt
     8101 tctaccatct tttctgatgc actctttgaa aagtcaaaca catgagtaag tgtagctgga
     8161 atctttccag aatcgattgc aagtgcaatc tcagttccaa tggcaccgca tccaagcaat
     8221 cctatctttt tcattttaat caaagctttg ttgcaagccc ttgttaaagc tataacttta
     8281 agcaagtttt gttcatataa tataaaatcg tgtttgaatg aattccaaca aatctggact
     8341 aatggaaaag tttctctttg gtatagcaaa gcagtggata gcaggagatt caatggagca
     8401 ggccatcgaa tccgccaggc ttgcaaacaa aaagggaatg gatgcaatca taaacaaact
     8461 aggcgaacat ctggcatcaa aaactcaggt tgaaaatact gtaaaagagt acatcatgct
     8521 tgtctcaaac ctgagaaaat caaaggtgga gggaggcata tcaataaagc caacacagct
     8581 tggactctca agaagcaaaa aggaatgcct gcaaaactat tctatagtaa ttgaaaaggc
     8641 tgcactctca caggcgtttg tatggattga catggaatca tcagaataca cagatgatac
     8701 tattgggatc tatcttgatt tgtttgacaa gtatgaaaga ttgggaattg ctatacaagc
     8761 aaatcttaga cgttcaagag gagatttgat gaggcttgtt gaaaaaggag caaaaatacg
     8821 tctagtcaag ggagcatatc gagaaaataa acaaattgca tttcaatcaa aaagcgaagt
     8881 tgatcaaaat tatcggaatt tgatggagat gctttttgta aaagcaaacg agtttggaat
     8941 tgcaacacac gattctgtct tgattgaaaa ggcaatcaaa ctatcaaaag cacatgaaaa
     9001 aaaattcgag tttcagatgc taaaaggaat acgcgacgag ctaaaaccta gtcttgtgaa
     9061 aaaaggattc tcaatatcag aatacattcc atatggaaca aactggttgc cttattctat
     9121 tagaagacta aaagaaagaa aacgtaacat cctacttttg ggaagctcct ttattcagtc
     9181 acacagagtc tgagtctgtt ctcgcataaa ctggagtaaa taatattctc cacctgcacc
     9241 cttgcctgaa attccagaat ctttccaacc tacaaatggt tgagccctga caagtgcacc
     9301 tgtagttgca cttgccgctc tattcgcata tgtgactcct gcctcgattt tatcaaaaaa
     9361 tgtattgatt tgatttttgt cttggctgaa tattcccgct gttagtccat aatctgtctt
     9421 atttgctaac ttgattgcct cctcaaagtt ctcaaactct gagacgcaga caaagggcaa
     9481 aaacaattca tctctgagaa gctcatgttc ttgtggaagt ccagtgacaa tagtaggctc
     9541 tgcaaaatat ccatccttgt attcttgatc tttgagtaca gaaccaccat ggactatttt
     9601 gccatctttt ttggcaatct ctgatacttt ttcaaatttc ttcaatgccg attcgttgat
     9661 aattggtccc aaaaagacat ccttttgcca tggcttgcca atcttgagtg attttgtttt
     9721 tgcaacaaat ttttccataa attgtgaagc aatatttttt tgtacataaa ctctagagca
     9781 tgcactacat ttttgtccac catatccaaa agcagctcgc ataactcctt ctgcagcttt
     9841 gtcaagatct gcagattctg ttacaattac aggattttta ccgcccatct ctgaaatgaa
     9901 tgggcgcgga ttttgttcaa taaatgttct aaatcctgat actccaacat ctctggaacc
     9961 agtaaacgca attccatcta caaggttact ttcaagtatt gtttttccaa caacacttcc
    10021 ggcacctgtg accaagttca aagctgcggg aggaagcttg tgataaactg cattcacaaa
    10081 tttgtatgca gaaagtggag tgtcacttgc aggcttgagt actacagtat tgcctgtaag
    10141 cagagcacca gtagtcattc ctattgcaat cgcagatgga taattgaacg gtgatattat
    10201 gccccaaaca ccatagggtt ttagcacgct gtgagttttc tcgtttggat tggcactctt
    10261 tgtctctttg gaataacctt ggtttgtctc aagcagttca gcataaaacc gcaaaaaatc
    10321 tattgcctca tcaatgtcac ccattgactc caatctgctt ttgccattct caaaagtcat
    10381 tattgctgca agtgtaaatt tttgtgaaga aaaatcatct gccacttccc tgaatatctg
    10441 aactcttttt tggtaaggtg tatcgcgcca tctgtgaaat gcatcctttg cagattctat
    10501 tgcatgaatg gtgtcttcct ttgttgccag gggaaagttt gctagtatga tgtttctgtc
    10561 tgctggggat cttacctgaa actggttttc agaatggatt tcttttccac ctattatcat
    10621 tgggtaactt ttgccaaaat cgttttttat tgactctact gccttgtcaa attttttatg
    10681 aaactcatct gtagtgttgt ttgatacagc tcttgaccat gtcttttcgt tttcaaacac
    10741 aggaattcta tgtgcccact caataaattg attctctatt agcttgtacg caaatcaatt
    10801 tcaaacgcga aacattttat ccacgtatgc aaaggatgct tatgcgaaat actgtttatc
    10861 ttgacgcttt aaaaaaacgt gaagagactc tatctatctt aaaggggcaa agatttgagc
    10921 agatattgca ggaagccaag aacaactggg tagaatacat tccgcaaaga caggattctg
    10981 taatagcagg aattgacagt agctacaaca gtacaaagtt tcagggattg gaactatggg
    11041 ttgttgcagc agtatcgata aaatctgatg gcacaatgat ttcagagctg cacaatcagg
    11101 gactaggaac cccaaatccg gagcttgctg cacaggcaag caagatggaa attgatgcat
    11161 gtactgcatc tgtggatgag gcagatgtag ttgcacttga tggttcacta tactcacaat
    11221 ttctcacaag acaagcctct cttattgcca tgcttacctc tgctataaag aagaaaaaca
    11281 acgtgatttt tgtatcaaaa acatctgtaa caaaaaatga gtttggacac cttggatcct
    11341 tggcaggaga tattttctat tacaatcatg cagtaaaaaa acctgggttt agcaaaatgt
    11401 ttgtagacaa agatctagga cctggcaaag tcatttcatc tgtttatgca agactgcgag
    11461 actctactcc gctgataaaa attgaattgt ttggttctga tcatgacgag tcagaaataa
    11521 aatcactttt ggacagactg tacaaaaaca gcgtctctgg atatccttat gcattaaagc
    11581 ttgcacacaa caactgtaag atctcttaca gtgatctggc aagactggtt agtttatacg
    11641 gtcttggaaa tgaaactggc tcaagggagg ttcttgaatg aatattggtg tagtaatagg
    11701 agagtcaaaa ccaactgaaa taactgcaca atcagtgcga ccactttctg ttggtgaata
    11761 tgtcattgtg agttcacaaa atggaaaact acttggtctt gtggaaagat caatcgtttc
    11821 aagtgatgca ttaactgacg tgcgaaactt tgatgaagca atggaaagca aagaagttgc
    11881 tgagctaaat tccagagata aaaactataa agtaaaaatt ggaatcttgg gctttttaga
    11941 taatctgcaa aaaggccaag caattgttcc tgcagtgccg cctcttccag gtacgtcaat
    12001 actggaagct acacaagaag atcttggaat aatctttgca ccaaaagaaa aagaatggat
    12061 tagaatcggt tcacttctta gaaattcaga aattgaggca aaaatcaacc tcaataaaat
    12121 tgtctcacgg cacataggaa tacttgccat gacaggcatg ggaaaaagca accttgtatc
    12181 attgatagca aaacaggtgg cggacttgca aggaactttg ataatttttg attatcacaa
    12241 tgattataca aatctgagtt tgccaaagat aagcgtcgca gatgccaaga taaatccaag
    12301 actattacag gcagacaagc ttgccgaagt aattgaattg cgtgaaaacg cagacaaaca
    12361 acaacgagtc ctgcgtgaat cgtttactgc atctgtacga caggcaaaag atttctggac
    12421 agcacttgaa gacagcgttt ctgaatttgg tcacaccaca aagggatatg ctgacagtgc
    12481 aagcagggtt cttgacaaaa ttgatgatgc aaaacacagg ttttctgata tcttggatcc
    12541 tgattgtggt gatccggtag atttgataaa agaaggcaga gtaaatgtgc taagcgttgc
    12601 agagctaagt gaaaggcagg caaacgtagg actatcgtac tatctgcaag agttgttaaa
    12661 tgacagaaag gattcctact gggcaaaaaa gggaaggaaa ctttcaagaa agaatacgaa
    12721 atttgcatca ccgctttttg taataattga agaagcacat gtctttattc caaaaggcga
    12781 acacactgac acaaaatact gggcatcaaa agttgcaaga gagggaagaa agtttggaat
    12841 tggacttggc atagtgtcgc aaagaccaag aaacatcgat cccaacgtgc taagccagat
    12901 gggctcactt gccatcatga agatagtcca agatgatgac cagcagcaaa ttgcctcagc
    12961 ggcagagtca ttgagtcgtg accttctcac acaactatca tccttgaaca taggtgatgc
    13021 agtgcttgtt gggcagtggg tcaacctccc atcgattgtt catgttgacg aagtaaaagg
    13081 aaagtcaata ggttctgatc ttaatgctgt agcagagtgg tcagaatcca caaaattcaa
    13141 agacattgca gcagaatcta cacaaggtct aattcaaaaa gacctgctta tggattgaga
    13201 tgatattttc acacatatct gacactcatt tgggatttgt acaatattat tcagaagaac
    13261 gcgagcagga tgtctatact gcatttgacc aggcaattga cacatcaatt aatgatcatg
    13321 ttgattttgt aattcttgca ggagacatat ttcatgtacc aaatccaagt ggaaaggcaa
    13381 tcttggtttt ggcaaatgca ctaaaaagac taaaacaaaa aaacattgat tcgtttttca
    13441 tactgggaga gcatgatatt agcaggatac gtgcaacgcc aattccgttt gtgtaccata
    13501 acttggaatt ttcaaaatac attggaaatg gaaaaccgct ttactacaaa gatgtgatgc
    13561 tggtaggctt tgacaagcga agaaaaagtg agataccaac gtttgaaaac gactttgcca
    13621 aggcagattc tgaagcaaaa aaacacacag gtcacaaaat tcttgtgttg caccaaggaa
    13681 tcggagaaat aaacaagttt gcaggcgagc taaactctac tgatctccca aaaaacttta
    13741 cttactatgc catggggcac cttcatgaga gagagctaaa aaagttcaat catctaggag
    13801 gcccacttgc atatcctggc tcaattgagc tgacatcaag tgagggaatc aaagaaacgc
    13861 aaaagggatt ctaccaagta gaccttactt catcggaagc aaaaccaaac tggataaagc
    13921 ttgacacaag accacaactt tcagtaaaaa ctgattttga aaacctaaaa aaagaagtag
    13981 acatgttggc agaaaagata caatcctttt cacgaaaacc tgtagttgaa ataaaaattc
    14041 atggagaaaa catagaaaca gatctggtcc agtcgcaaat tgcaagactg cagccacttg
    14101 cactacgatg cttttggaaa gcaatacggg aagaagaaaa ctctggcttg acatttgttg
    14161 acagacctgc aaaaattgat gatgaaatgt tcaagcttgc aaaagacatt ttgaattcag
    14221 agccatgggc aaactttaca ataagagatc tcttgccctt gctttcgaca aaccaaattg
    14281 atgaagctaa tcatcttgtg atggaaaact ttgttcgttt taaaaaggag aaaaaatgtt
    14341 aactgaaatt gagatgggaa acttcctgtc acactctaac acaaaactgc catttgacaa
    14401 aggagttact gtgtttgtgg ggcacaacgg tgcaggaaaa tcaagcgtta tcgatgctgt
    14461 tacctttgcg ctctttggac agcacacaag aaactcaaac aaaggccttg ttaggcgtgg
    14521 ctcaaaccaa gcatatgcca aagttgactt taccatctca aacagagcat ttgaggcagt
    14581 acgaaaaatt gattcaaaag gaacactctc tgcgcagctt tttgagaaaa aagacggaag
    14641 cctagttcct cttgcggcag gagaaagaaa gcagtttgga gaatcaatga caaaagaaat
    14701 agaatcgtta attggaatgg attttgacaa gctaaaaatc gcgtcaatcg tgcgacaggg
    14761 ggaactaaat tctataattg acgcagatcc aaaaaaattc aaagaactag tcaatacaat
    14821 aattggaatt gacaagcttg acactgcgtt tggcttgatg aaagatgttg tagaaaactt
    14881 tcgaagctca ataagaaagg atctagagta tgatgatact gacattgaaa gcctgagatc
    14941 aaatcttgat gtgattacaa aagaaattca ggtagcagaa ccacaaaaag aagaattgac
    15001 tataaaaaag caaaaacaag aacatgaaat aactttactt cagaaaaaag ttgatgccga
    15061 gtccccaaag gaatcaaaaa ttaaagaact tgacacaaga aaatctgagc ttgtaagata
    15121 cgtcaaaact gcaatagcat cacttcaaaa agatgtttta gaaaaagaac gaaagctttc
    15181 tgattgtgaa ggctgctttg atcatctttt ctctaaaaaa gaaattgaag cacaaatgga
    15241 aaaaattgaa gctaacctag agtcgacacg aaatgaaatc aggcaactat cagaaaaggt
    15301 tggaaacctt ggaggccaag aggaacttgc ggcaaaacta aagctaaaag acggcaaatg
    15361 cccagtgtgt gattccgta
//
LOCUS       DATIDL010000086         3367 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrosopumilaceae archaeon isolate SMAG_U16342
            k141_712730, whole genome shotgun sequence.
ACCESSION   DATIDL010000086 DATIDL010000000
VERSION     DATIDL010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935836
            Sequence Read Archive: SRR25158326
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosopumilaceae archaeon (soil metagenome)
  ORGANISM  Nitrosopumilaceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrosopumilales;
            Nitrosopumilaceae.
REFERENCE   1  (bases 1 to 3367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/04/2024 05:09:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,520
            CDSs (total)                 :: 1,474
            Genes (coding)               :: 1,474
            CDSs (with protein)          :: 1,474
            Genes (RNA)                  :: 46
            rRNAs                        :: 1 (5S)
            complete rRNAs               :: 1 (5S)
            tRNAs                        :: 43
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3367
                     /organism="Nitrosopumilaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_712730"
                     /isolate="SMAG_U16342"
                     /isolation_source="soil"
                     /db_xref="taxon:2202732"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..221)
                     /locus_tag="VLB45_03825"
     CDS             complement(<1..221)
                     /locus_tag="VLB45_03825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04865.1"
                     /translation="MNFKTILAISLIASVLWVPTGYATKTFADEPVLDTDSVNEVGLR
                     VAFHFRETTEDVNTFKNFKPFSQTATVTST"
     gene            358..1326
                     /locus_tag="VLB45_03830"
     CDS             358..1326
                     /locus_tag="VLB45_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSD04866.1"
                     /translation="MDEILLVQNLKKYFIKKGIFYSSKTTVKAVDDVSFSLKKGEILV
                     LAGESGSGKTTLARLILRSIEPDSGKVFFNGMEIDHDKKNLEKIRMQCQMIHQDPYAS
                     INPRMRVSDIIMEPLEIHKIGTKQERKKMILDVLSEVKLEPAEEIFNKFPHMLSGGQR
                     QRVVLARALVMKPKLIIADEPVSMLDVSVRAEILELMRELQQKHEISYIYITHDLSTA
                     RYFGQKIAIMYLGRIVESGHIDDVLLHPRHPYTQALIDAISEPNPENLLQEKKIRINE
                     PLDIDVYQGCRFRARCPYAIEKCKEDPQLEQAGQDRLAACFVKLES"
     gene            complement(1349..1654)
                     /locus_tag="VLB45_03835"
     CDS             complement(1349..1654)
                     /locus_tag="VLB45_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04867.1"
                     /translation="MHKDRGTCKDICKEYKVRKPRDGTSRYLSGQVRCQICGVFISRE
                     GCHDREEHPATDDTVDLYCNCCNCMVRSKPRNKPGMKPTKKTPQEEYDELKKSLGNN"
     gene            complement(1721..>3367)
                     /locus_tag="VLB45_03840"
     CDS             complement(1721..>3367)
                     /locus_tag="VLB45_03840"
                     /inference="COORDINATES: protein motif:HMM:NF014516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HSD04868.1"
                     /translation="LVDSKESAYSISKKGYKAVTSDGQFFEADANAVVVDVDSKISKL
                     TKIILLSTSVDDIISALNLLKKFIQNKKTQLKKIERITKSLDDRYHTSETGLANVDLN
                     FTDIKAKLKSISLTEDQLTSRISHLTKKDEVIKTGAAKLESYISSLEERISVTQENYA
                     EEEQARIASALAELNEKKSVLLAAQSSVMTQFREQTAQLTMLTNDENALKSNTRMLSQ
                     EQSTLNHEKHDLEVQSRALTKEKEIADAGLISLREKEQQLISTSGTSVSTLKEYDAKL
                     KERYDQEKSITKEINNFERQSDSLSRDIKDLTENEAKIRTALNSYGYDEILETFEVDQ
                     ILSTLESEKKALTGTLNTKSPEAYVEISNGYRSMSTRKNELEEERNSIVRFIEEIDKD
                     KRQTFLEAYDKVDQDIRKVFNTMTGGNAWLEIQNEDDIFSSGLSYLIQFPNKQKREST
                     SISGGEKTLAAITFLLALQTLKPSPFYLLDEVDAHLDALNTERLGKILEERAKGNQII
                     MVTLKDSTVQRAQLIYGVYPKGGVSQVVTYKQSNVPPVAN"
BASE COUNT          978 a          601 c          629 g         1159 t
ORIGIN      
        1 gttgaggtca cagtagcagt ctgtgagaat ggcttgaagt ttttgaatgt gtttacatct
       61 tcggttgttt cacgaaaatg aaatgctaca cgcaatccaa cctcatttac gctgtcagtg
      121 tcaagcactg gttcgtcagc aaatgtcttt gttgcatatc ctgtaggaac ccacaggact
      181 gatgcaataa gagatattgc tagaattgtt ttaaagttca ttcttagatg aactcaacat
      241 catagattaa taattaagat ccgtatagtt cacttatctt ctatatagat atagagttaa
      301 ttcaattcat gtagaaaaac cagatctctt gatatagttt ggattacaat gatagatgtg
      361 gatgaaattc ttttagtaca aaatctgaaa aaatatttta ttaaaaaagg gattttctat
      421 tcatcaaaaa caacagttaa ggcagttgat gatgtgtcgt tttctttaaa aaaaggtgag
      481 atcttagtac tagctggcga atcggggtct ggcaaaacca cgcttgcaag actaatttta
      541 agatcgattg aaccagactc tggtaaagta ttcttcaatg gtatggaaat tgatcacgat
      601 aagaaaaacc ttgaaaaaat aagaatgcaa tgtcagatga tacatcaaga tccttatgct
      661 tcgataaatc cgcgtatgag agtatcagat attataatgg agccacttga aattcataaa
      721 attggaacaa aacaagaaag aaaaaaaatg attcttgacg tactcagtga agtcaaacta
      781 gaacctgctg aggaaatttt taataaattt ccacacatgt tatctggcgg acaaaggcaa
      841 agagttgttc ttgcaagagc ccttgtaatg aaaccaaaac tcatcattgc agacgaacca
      901 gtttctatgc ttgatgtttc tgtccgagca gaaattctag aactgatgag agagttgcaa
      961 caaaaacatg aaatttcata tatttacatc acacatgatc tatcaactgc aagatatttt
     1021 gggcaaaaaa ttgcaatcat gtatttggga aggattgtag aaagtggtca tattgatgat
     1081 gtgcttttgc atccaagaca tccttatacg caggccctaa tcgatgcaat ttcagaacca
     1141 aaccctgaga atcttttgca agaaaaaaaa atcagaatta acgaaccact tgatattgat
     1201 gtatatcaag gatgcagatt ccgtgccagg tgcccttatg ctatagaaaa atgcaaagaa
     1261 gatccacagc ttgagcaagc aggacaagac agattagctg catgttttgt caaattagag
     1321 agttaaaaat gaacggcgaa gaaaacgtct aattattccc taaactcttt ttcaattcat
     1381 cgtattcttc ttgtggagtt tttttagtgg gtttcattcc aggtttgttt cttggctttg
     1441 atctcaccat gcagttacaa cagttacagt acaaatctac agtgtcatca gttgcagggt
     1501 gttcttctct gtcatgacat ccttctcttg agatgaaaac tccacaaatc tgacatcgta
     1561 cttgacctga caaatagcgg ctagttccat ctcttggctt tctaacttta tactctttac
     1621 aaatgtcttt acaagtaccc ctgtccttgt gcatacttaa ttcctaacaa agaagaataa
     1681 attctcatgc aaatgagtgg ttttgtattt taaagtaaga ctaatttgca acaggaggca
     1741 cgttagactg cttgtatgtg acaacctgtg aaactccgcc tttcggatat acaccgtaga
     1801 taagttgtgc tctttgcact gtagaatcct ttaatgtgac catgataatc tggtttccct
     1861 ttgcacgctc ttctagaatt ttccctagtc tctcagtatt tagtgcatca aggtgtgcgt
     1921 ctacctcatc aagcaagtaa aatggcgagg gtttcaatgt ctgtagtgcc aaaaggaatg
     1981 tgattgcggc aagtgtcttt tcaccaccac taattgaagt agattctctt ttctgcttgt
     2041 ttggaaattg tatcaagtat gacagtccag atgaaaatat gtcatcttca ttttgtattt
     2101 caagccaagc gttgccacca gtcattgtgt tgaacacctt tcgaatatcc tgatctactt
     2161 tatcgtaggc ctccaaaaat gtctggcgct tgtctttgtc aatttcttca atgaatctta
     2221 caattgaatt tctttcttct tcaagttcat ttttacgggt agacattgag cgatacccgt
     2281 ttgaaatttc aacatacgcc tctggagatt ttgtgtttag cgttccagtc aatgctttct
     2341 tctctgattc aagtgtggat aagatttgat ccacctcaaa agtttctaaa atttcatcat
     2401 atccgtatga atttaatgcg gtacgtatct tggcttcatt ttcagtaaga tccttgatgt
     2461 cacgcgaaag ggaatcagat tgtctttcaa agttattgat ttcttttgta atagatttct
     2521 cctgatcata tcgttctttt agtttggcat cgtattcttt tagcgtagac acagatgttc
     2581 ctgaagtgga tatcagttgt tgttctttct ctcttaaaga aatcaaacct gcatctgcga
     2641 tttccttttc ttttgtcagt gcgcgtgatt gcacttcaag atcgtgtttt tcatgattta
     2701 gtgtagattg ttcttgcgac agcatacgcg tgtttgattt tagggcgttt tcgtcgtttg
     2761 taagcatggt tagttgggca gtttgttctc gaaactgagt cataacagag ctttgggctg
     2821 ccaataatac agattttttc tcatttagtt ctgcaagcgc acttgcaatt ctagcttgtt
     2881 cttcttccgc atagttttct tgtgttacag aaatacgttc ttccagcgaa ctaatatagg
     2941 attcaagttt ggcggctcca gtctttatga cttcatcttt ttttgtaaga tgtgaaattc
     3001 gtgaagtgag ttggtcctca gttaatgaaa ttgattttag ttttgccttt atgtcagtaa
     3061 agttcaggtc cacattagca aggcctgttt ctgatgtatg gtatctgtca tcaaggcttt
     3121 ttgttattct ttcaattttt ttaagctgag tctttttgtt ttgtatgaat tttttaagta
     3181 aattcaatgc acttatgata tcatcaactg aggtgctaag caagattatc tttgttagtt
     3241 ttgatatctt tgaatcaaca tcaactacca ctgcatttgc atcagcttca aagaattgcc
     3301 catcagatgt tactgccttg tatccttttt ttgatataga gtaggcagat tcttttgaat
     3361 cgacaag
//
LOCUS       DATIDL010000087         6663 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrosopumilaceae archaeon isolate SMAG_U16342
            k141_1116193, whole genome shotgun sequence.
ACCESSION   DATIDL010000087 DATIDL010000000
VERSION     DATIDL010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935836
            Sequence Read Archive: SRR25158326
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosopumilaceae archaeon (soil metagenome)
  ORGANISM  Nitrosopumilaceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrosopumilales;
            Nitrosopumilaceae.
REFERENCE   1  (bases 1 to 6663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/04/2024 05:09:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,520
            CDSs (total)                 :: 1,474
            Genes (coding)               :: 1,474
            CDSs (with protein)          :: 1,474
            Genes (RNA)                  :: 46
            rRNAs                        :: 1 (5S)
            complete rRNAs               :: 1 (5S)
            tRNAs                        :: 43
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6663
                     /organism="Nitrosopumilaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1116193"
                     /isolate="SMAG_U16342"
                     /isolation_source="soil"
                     /db_xref="taxon:2202732"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..517
                     /locus_tag="VLB45_03845"
     CDS             <1..517
                     /locus_tag="VLB45_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016939502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04869.1"
                     /translation="LYRIGEHDVYFIPVYTAGSGGVVTQLGTIAAVGAAFDGEYYVGM
                     GNTPQQAFAAYLAKLSGVAPSTTGGYVELDRDSRIQTLKSLFEKQNLKIVSPTSVQIP
                     LSFEEGKISFFQESDREDTEKLISNFIENFVKPRSDRIIVWEEDNAINFGTVVIIENV
                     PELHYISIEVG"
     gene            680..1048
                     /locus_tag="VLB45_03850"
     CDS             680..1048
                     /locus_tag="VLB45_03850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04870.1"
                     /translation="MSFINKNKMKTVTIVIMITFFGLFVTGTGFQMADAAGPSKRLHL
                     TVLEPTGQPSFAFCKVITDVDTLPQFATTTKRGTIHFVVSSTSTIAFVTCSSPTSFID
                     SFPIPLPVHTNRVTISLITP"
     gene            1112..1654
                     /locus_tag="VLB45_03855"
     CDS             1112..1654
                     /locus_tag="VLB45_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962336.1"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HSD04871.1"
                     /translation="MLLVIDNGSVYTKSLLDFLFEKKIKFLDLTFDKVNFQELHRYSS
                     IILSGRRKNNLDMNVVNSKIIKHAVAEKKHLLGICYGAEILALTLGGTLKKMDKLHKG
                     NQEIEILKTNPLCSGKINVFESHSYMISKIGEIFSPIAKSDICNFEIIQYDNLSIFGT
                     QFHPEMSDDGKLLLEKFVAI"
     gene            1689..2639
                     /locus_tag="VLB45_03860"
     CDS             1689..2639
                     /locus_tag="VLB45_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSD04872.1"
                     /translation="METEHMLLLPVVEEDEENICLPLVITVVSKYWGIDLPLLEAKEI
                     AKKYPNMKGSIMIEGIELAERHGLTSLILNSSIKELKKIIDMGIPPIVILPGIHETVQ
                     HASVISGYDETEKAIIHYFPQPDTVGVIPEKKFDQQWAEDGRLMLLVAPPDILSKINP
                     ENIKVEKSNRFCFISERLRLQKKLDDAINALRKALEIDQKNSTAFCLLGGMLNDQNSS
                     EAVQYYQQSIEYNKSCYLAYRGLGNYYLKIKDYPNAEKYYTLAIEINPNRFAPIYKNR
                     GLARFEQGKKSEAKEDFETYLEQIPNAADKNNILDAIKEL"
     gene            2685..3098
                     /locus_tag="VLB45_03865"
     CDS             2685..3098
                     /locus_tag="VLB45_03865"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010195459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HSD04873.1"
                     /translation="MDCIFCNIIKGKIPARKIIETEKSLAFLDAFPLTKGHVLVIPKN
                     HYTKIQEMSKEDNTDLFEIVRILTNKIENLFSPSLIAVHNGRESGQEVPHVHVHIIPR
                     STSDGAGPVHSMFEKRPKFTNDEFDQIAKTLRDEN"
     gene            complement(3111..3184)
                     /locus_tag="VLB45_03870"
     tRNA            complement(3111..3184)
                     /locus_tag="VLB45_03870"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3148..3150),aa:Gly,seq:ccc)
     gene            3237..3410
                     /locus_tag="VLB45_03875"
     CDS             3237..3410
                     /locus_tag="VLB45_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-domain-containing protein"
                     /protein_id="HSD04874.1"
                     /translation="MDAKCPQCEKIATLDDNITIVKCIHCGFEAKYDDYLEIMKEKVG
                     ELVANFNPDRSGF"
     gene            3610..4023
                     /locus_tag="VLB45_03880"
     CDS             3610..4023
                     /locus_tag="VLB45_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S6e family ribosomal protein"
                     /protein_id="HSD04875.1"
                     /translation="MATFKMIVSDKKGKSLTRELKEKDANPLLGLLVGAEVDAAIVGQ
                     PGKMKITGGSDKSGVPMRADIHGGARKYVMLSKGVGLQDAEKGQRFRKLIRGNIITEE
                     IYQINGVLDGELKIDEAAQTAPEEKQDKAEKPKKA"
     gene            4055..5320
                     /locus_tag="VLB45_03885"
     CDS             4055..5320
                     /locus_tag="VLB45_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007549363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 subunit
                     gamma"
                     /protein_id="HSD04876.1"
                     /translation="MHWRETLPDWYIKKYGHQPCVNIGTAGHVDHGKTTLIQALTGVW
                     TSAHSEELKRGITIRVGYADTAFYKCKSCEPPLGYSVSPKCNNCGKESELSRVVSFVD
                     SPGHESLMANMLSGSALIDGAMLVIAANEKVPQPQTKEHLLALQTLGIQQIVLVQNKA
                     DLISYEDAIANHADIVKFVKGTNAAKAPIIPISAQSKLNIDALIGAIESTIETPKRDE
                     SKNTVMHVLRSFDVNKPGTKIKNIKGGVIGGSITQGSLKVGDEIEIKPGLANEKNGAY
                     ESIVTEISSLGTGAGLVDEVKPGGLVAIGTQLDPSMTRSDSLIGSVIGKPNTLPENSS
                     SAKIEVNLFDTAVGSVDEIKVSSIQVGESLRLSIGTAPILSKVTSVRGEKMDVQFRRP
                     VCLFEKGRVAISRRIAERWRLIGAGVVLG"
     gene            5313..5507
                     /locus_tag="VLB45_03890"
     CDS             5313..5507
                     /locus_tag="VLB45_03890"
                     /inference="COORDINATES: protein motif:HMM:NF037434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04877.1"
                     /translation="MVEVICDTSFLMILASEQIKNISNLETEIGTIEFVVPNMVISEL
                     EKLSQSTNNKKKDLHLMPLK"
     gene            5717..6373
                     /locus_tag="VLB45_03895"
     CDS             5717..6373
                     /locus_tag="VLB45_03895"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HSD04878.1"
                     /translation="MFEYDGIKIKWYGHDTFTIEKKITICIDPYKLSKPIHADIVLIS
                     HNHFDHLSLEDLSKVSSSDTTIIAANECIAQISKIQSKEKIGVVPGQEKIVKEIKVKA
                     VPAYNIDKINPDTSKPFHPKDDKKVGFVINIEGTSIYHTGDSDLIPEMDDLRPDVLLV
                     PVSGTYVMTAREAAKATEKIKPKVAIPMHYGTLVGTENDAKEFKNMVKSCQVQILTKE
                     "
     gene            6504..>6663
                     /locus_tag="VLB45_03900"
     CDS             6504..>6663
                     /locus_tag="VLB45_03900"
                     /inference="COORDINATES: protein motif:HMM:NF014565.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004576 - oligosaccharyl transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STT3 domain-containing protein"
                     /protein_id="HSD04879.1"
                     /translation="MIRSQAADYGFELNEFDPFFNYRATQYLVENGIDAYVNWHDDMS
                     WHPVGRDVF"
BASE COUNT         2421 a         1138 c         1246 g         1858 t
ORIGIN      
        1 tctatataga attggtgaac atgatgttta ctttattcct gtttatacag ctggttctgg
       61 tggagttgtg acacagcttg gaacaatagc tgctgttggt gcagcttttg atggagaata
      121 ttatgttggt atgggtaaca cgccacaaca agcctttgcg gcatatctag caaagttatc
      181 tggagttgca ccatccacta ctggaggcta tgtggaactt gatagggatt ctaggataca
      241 gacattaaaa tcattatttg aaaaacaaaa cctcaaaatt gttagtccaa catcagtaca
      301 gattccatta tcgtttgaag aaggcaagat ttctttcttc caagaatccg atcgtgaaga
      361 taccgagaaa ttaatctcta actttataga gaattttgtc aagccacgaa gcgatagaat
      421 aattgtttgg gaagaagata atgctattaa ttttggtact gtggtaatta tcgaaaatgt
      481 tcctgagcta cattatatct ctatagaagt aggataattc cactttttga taaaatagca
      541 acttctcatc aataatacta gaattgggat ctaaccagac tcctattatg aactagtatt
      601 agcaaccaag attcaacaac tattcagaac atacgtaaaa tcaacaaaac cgaaaattta
      661 cttagatcaa gtataaataa tgtcattcat taataaaaac aagatgaaaa ctgtaacaat
      721 tgtcataatg ataactttct ttggattatt tgtaactggc acaggttttc aaatggcaga
      781 cgctgctgga cctagtaaga ggcttcatct tacagttcta gaacctacag gtcaaccatc
      841 ttttgcattt tgtaaagtaa taacagatgt tgatacccta cctcagtttg ctactacaac
      901 taaaagaggt acaattcatt ttgttgtgtc ttctacttct acgatcgcat ttgttacttg
      961 ttcaagtcca acatcattta ttgattcatt tcccatacca ctcccagttc acaccaacag
     1021 agtgacaata tctctaatca ctccatagtt atttatcaca catgtggaaa tcatagaata
     1081 gaaccatata tcttaactaa ctacacagag catgcttctg gttatagata atggctcagt
     1141 atacaccaaa tcactcttag attttttatt tgaaaaaaag ataaaatttt tagatcttac
     1201 atttgataaa gtaaatttcc aagaactgca tagatattct tcaataatcc tatctggcag
     1261 aagaaaaaat aatctcgata tgaacgtcgt taattcaaaa ataatcaaac atgctgtggc
     1321 agaaaaaaaa catctccttg gaatatgcta tggtgcagaa attcttgctc taactcttgg
     1381 tggtacacta aagaaaatgg acaaactaca taagggaaat caagaaattg agatactaaa
     1441 aacaaatcct ctttgtagtg gaaaaatcaa tgtatttgaa agtcattctt atatgatatc
     1501 aaaaataggt gagatttttt ctcctattgc taaatctgat atttgcaatt tcgaaataat
     1561 tcaatatgat aatctgagta tatttggcac ccagttccat ccagagatga gtgatgatgg
     1621 aaaactcctc ttggagaaat ttgttgcaat ttgatggttt taatatctaa catccaatag
     1681 taagtttctt ggaaactgaa catatgcttc ttttaccagt tgtagaagaa gacgaagaaa
     1741 atatttgcct accacttgtt atcacggtgg tttcaaaata ttggggaatt gatttaccac
     1801 ttttagaagc aaaagaaatt gcaaaaaaat atccaaacat gaagggaagc ataatgattg
     1861 aaggaattga gcttgcagaa agacacggct tgacaagctt aatcctgaat tcatcaatta
     1921 aagaactaaa aaaaatcata gacatgggaa ttccaccaat tgtaattctt ccaggtatac
     1981 acgaaacagt acaacacgca tctgttatct ctggttatga tgaaacagaa aaggcaataa
     2041 ttcactattt tccgcaacct gatacggtag gagtcattcc agagaaaaaa tttgatcaac
     2101 aatgggcaga agatggtaga ttgatgctac ttgtagcccc tcctgacatt ctctcaaaaa
     2161 taaatccaga aaacatcaaa gtggaaaaat caaacagatt ctgtttcatc tcggaaagac
     2221 tgcgtttaca aaagaaactt gatgatgcaa taaacgcact aagaaaagca cttgaaatag
     2281 atcaaaaaaa ctcgaccgca ttttgtcttc ttggaggaat gttaaacgat caaaattcct
     2341 ctgaagcggt acaatattat cagcaaagca tagaatacaa caaatcatgt tatcttgcat
     2401 ataggggtct tggcaattat tatctaaaaa taaaggacta tccaaatgca gaaaaatact
     2461 atacgctggc cattgagata aatccaaaca gatttgctcc gatttataaa aaccggggtt
     2521 tggcaagatt tgagcaagga aaaaaatctg aagccaaaga agacttcgaa acttatttgg
     2581 aacagatacc caatgctgca gacaaaaaca acatacttga tgcaataaaa gaactctaat
     2641 ttatttacat aagagtttta caatactatt ccactgatga tcacatggac tgcatttttt
     2701 gtaatataat aaaaggaaaa attccggcac gaaaaataat tgaaacagaa aaatcactgg
     2761 catttttgga cgcatttccg ttaactaaag gccacgtatt ggttattcca aaaaaccatt
     2821 acactaagat tcaagaaatg agtaaagaag acaacacaga tctttttgaa atcgtgagaa
     2881 ttttaacaaa caaaatagaa aacttatttt cgccttcgct tattgctgtc cacaacggaa
     2941 gagaaagtgg acaggaagtg cctcacgtgc atgtacacat aattccgcgc agtactagtg
     3001 atggtgcagg accagttcat agcatgtttg aaaaaagacc aaagtttaca aatgatgaat
     3061 ttgatcaaat cgcaaaaaca cttcgtgacg aaaactaact atgtattaag tgcggagtgc
     3121 gggatttgaa cccgcggcct tcagcttggg aagctgacgt cgtgccgggc tggactaact
     3181 ccgcctaatt ccaactttga atttaacaat atatagtcta attactaaaa gaatacatgg
     3241 atgcaaaatg tccccaatgc gaaaagattg caacacttga tgacaacatt acaattgtca
     3301 aatgtattca ttgcggtttt gaagcaaagt atgatgatta cttggaaatc atgaaggaaa
     3361 aagttggtga attagttgca aactttaatc ctgaccgatc tggattttaa attaccaaaa
     3421 actaccttta tctgaacaat caagatgtgc accacagttt gtgcaaacca tgtgacaagc
     3481 ttgcattgag atcatcgggg tttcacattt gggacacttg atttcttcca tgatcttatt
     3541 tcgcacataa ttattttaag tatttaggta caaagattaa ttaatagtct gaagactttt
     3601 tcaaaatgct tggctacatt caagatgata gtatctgata aaaaaggtaa gtcgctaaca
     3661 agagaactaa aagaaaagga cgcaaatcca ctcttaggtc tactggtagg tgctgaagta
     3721 gatgcagcta tagttggaca acccggaaag atgaaaataa caggtggaag cgacaagtca
     3781 ggcgttccaa tgagagctga cattcatggt ggtgcaagaa aatatgtcat gctgtcaaag
     3841 ggagttggtc tgcaagatgc agaaaaagga caacgcttta gaaaactcat ccgtggtaac
     3901 ataataacag aagaaatcta tcagataaac ggcgtattgg atggcgagtt aaaaatagat
     3961 gaagctgcac aaaccgcacc tgaagaaaaa caagataaag ccgaaaaacc aaaaaaggca
     4021 taagttaaca taaccgattt tcaaaacttg gatattgcac tggcgtgaaa ctcttccaga
     4081 ttggtacata aaaaaatatg gacatcaacc ttgcgttaac attggaaccg caggtcatgt
     4141 cgatcacggt aaaacaacac tgattcaggc tctcactgga gtatggacta gcgcacacag
     4201 tgaagagctg aaacgtggaa tcacaatacg tgtagggtat gcagatacag cattttacaa
     4261 atgtaaaagt tgtgagccac cacttggata ttctgtttcc ccaaagtgta acaactgcgg
     4321 aaaagaaagt gaattatcaa gggtggttag ttttgtagac agcccaggcc acgagagcct
     4381 tatggcaaac atgctttctg gttctgcttt aattgatggt gccatgcttg taattgcagc
     4441 aaatgaaaag gtaccgcaac cgcaaacaaa agaacacctg cttgcactac agacactagg
     4501 aatacaacaa atagtgctag tacaaaacaa agcagatctg atttcatacg aagatgcaat
     4561 tgcaaatcac gctgatatag taaaatttgt taaaggcact aatgctgcaa aagcaccaat
     4621 aattccaatt tctgctcaat caaaactaaa catagatgca ctgataggcg ctatcgagtc
     4681 tacgattgag acaccaaaaa gggatgaatc aaaaaacacg gtaatgcatg tattaagatc
     4741 atttgatgtt aacaaacctg gcactaagat aaaaaatatc aaaggtggtg taataggtgg
     4801 aagcataaca caaggcagtc tcaaggtagg tgacgaaatc gagatcaaac caggacttgc
     4861 aaatgaaaaa aatggagcat acgaatcaat tgtaacagaa atttcttctc ttggaacagg
     4921 cgcaggtttg gttgatgaag taaaaccggg aggccttgtt gcaataggaa cacagcttga
     4981 tccttctatg accagaagtg attcattgat tggttctgtt atcggcaagc caaacacttt
     5041 acctgaaaac tcctccagtg caaaaattga agtcaatctg tttgatactg cagtaggatc
     5101 agttgacgaa atcaaagttt catctattca ggtaggagag tcactacgat taagcatagg
     5161 tacagctcct attctttcca aagtgactag tgtaagagga gaaaaaatgg atgtacagtt
     5221 tagaaggcct gtttgtcttt ttgaaaaagg cagagttgca attagcagaa gaattgcaga
     5281 aagatggaga ttgattggag caggtgtggt acttggttga agttatttgt gatacaagtt
     5341 ttttgatgat tctggcatct gaacaaataa aaaatatttc aaaccttgaa acagagattg
     5401 gaaccataga atttgttgtt cctaatatgg ttataagcga acttgagaaa ctttctcaaa
     5461 gcacaaataa taaaaaaaaa gatctgcatc tgatgccctt aaaataatag attctttcaa
     5521 aaaaattgaa atctcaggaa aaaatgttga tgatgctatt atacgccatg taaggaatca
     5581 tagaggaata gttgctactg tagatatgga gctcaaacaa aagataaaaa actatggtag
     5641 cgtactttcg cttgcaaaca acaggattgt tttagagcca agtaaaactt aacttgaaca
     5701 aaaattaatt ctagctatgt ttgagtatga tggaattaaa ataaagtggt acgggcatga
     5761 cactttcaca atagaaaaga aaatcaccat atgtattgat ccatacaaac tttccaaacc
     5821 aatacatgca gatatcgtat tgatttcgca caatcacttt gaccatctga gcttggagga
     5881 tcttagtaaa gtctcatcat cagatacaac aataattgcc gcaaatgagt gtattgcgca
     5941 aatttcaaag atacaatcaa aagaaaaaat aggtgttgtg ccaggacaag aaaaaatagt
     6001 aaaagaaatc aaggtaaaag ctgtgcctgc atataacata gacaagataa atcctgatac
     6061 aagtaagccc tttcatccaa aagatgacaa aaaagttggc tttgttataa acatagaagg
     6121 tacgtctatc tatcatactg gtgactcgga tcttattcca gagatggatg atcttaggcc
     6181 tgatgtttta cttgttccag taagtggaac atatgtaatg actgcgaggg aagcagcaaa
     6241 agcaactgag aagataaaac ccaaagttgc tattccgatg cactatggta ctttagtcgg
     6301 tactgaaaac gatgcaaagg aattcaaaaa tatggtaaaa tcatgtcaag tacaaatcct
     6361 tacaaaagag taataagact aacaaaattc ataaaatttg attgattcct aataccagat
     6421 tatttcaggt aagcaacttt gaatttcgct tacatcattt acttgttatt ggaattcttg
     6481 caatttcttt ttcaatctct ttcatgatca ggtcgcaggc agcagattat ggatttgagc
     6541 taaatgagtt cgatcctttt tttaattata gagctacgca atacctcgta gaaaatggaa
     6601 tagacgctta tgtcaactgg cacgatgata tgagctggca ccctgttggc agagatgttt
     6661 ttg
//
LOCUS       DATIDL010000088         3438 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrosopumilaceae archaeon isolate SMAG_U16342
            k141_1523563, whole genome shotgun sequence.
ACCESSION   DATIDL010000088 DATIDL010000000
VERSION     DATIDL010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935836
            Sequence Read Archive: SRR25158326
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosopumilaceae archaeon (soil metagenome)
  ORGANISM  Nitrosopumilaceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrosopumilales;
            Nitrosopumilaceae.
REFERENCE   1  (bases 1 to 3438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3438)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/04/2024 05:09:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,520
            CDSs (total)                 :: 1,474
            Genes (coding)               :: 1,474
            CDSs (with protein)          :: 1,474
            Genes (RNA)                  :: 46
            rRNAs                        :: 1 (5S)
            complete rRNAs               :: 1 (5S)
            tRNAs                        :: 43
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3438
                     /organism="Nitrosopumilaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1523563"
                     /isolate="SMAG_U16342"
                     /isolation_source="soil"
                     /db_xref="taxon:2202732"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..594
                     /locus_tag="VLB45_03905"
     CDS             <1..594
                     /locus_tag="VLB45_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSD04880.1"
                     /translation="VLKQHHYKGNYDDIISEMVNSVGLDISVLKKYPHELSGGMKQRI
                     VIAMALLLKPKFVIADEPTTALDVLVQAQIINLLKKLKKDGMSIMLITHDLAIISEIA
                     EKIGIMYAGKLVEFGNASEIYKNPKHPYTQALLESIPSLKIKKEIKYIKGNPPNLLNP
                     PEGCRFYDRCPHAMEKCKKDPPRFKTNSGYVHCWLYE"
     gene            complement(617..1261)
                     /locus_tag="VLB45_03910"
     CDS             complement(617..1261)
                     /locus_tag="VLB45_03910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04881.1"
                     /translation="MKKSTLQRFVAIAMIASVLWIPTGYATKTFADEEPVLDTDSVNE
                     VGLRVAFHFREATEDVNTFKNFKPFSQTATVTSTTVTTSGFDTLKSVPKFVLEKVVGW
                     DTPYLYHAVDESHKWGKTFPHDYSEFDVDVTFQRGGAAFRQLFYTDCQVTDYYIYTDF
                     DKEETYNAKTDFVYLDSFTFECRGVNYGNPVYDQISVNAKMQERAELEAQRNAQ"
     gene            complement(1404..2831)
                     /gene="pstS"
                     /locus_tag="VLB45_03915"
     CDS             complement(1404..2831)
                     /gene="pstS"
                     /locus_tag="VLB45_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HSD04882.1"
                     /translation="MKIKKILLGLILILVLIVPTTSLDANAVLKPAEPKGQFQITGAG
                     ATFPFPLIDKWRVEYNELYSGITLNYQSIGSGGGVKQHTEKTVDFGASDAPLTPAESG
                     IAVGTLHIPESIGAVTISYNIPEYAKSGLKLTGDVIADIYMGKITKWNGKKIQDLNKD
                     VVLPNKDILVVRRSDGSGTTFVFTDYLSTVSKTWDTNIGKGKSIGWPVGVGSPGNEGV
                     AGVVRKTPYSIGYVELAYAVQSEMTFAFVQNADKTNFVEPSLESTKAAAAASVTKLPK
                     SDGDWSKVSIVNQPGKNSYPISSFTYLLVYQDLGKIKGMDMEHAKALVHLIHWMITDG
                     QEHAPSLLYVPLPDEVRELGKQGLAKIKFEGQQLFAYDGSGIVSTKPTSESTYNIPPW
                     IKNNAKWWAENQISDGDFVMGIQYLVKEKIMIIPETKTGTATSSQQIPSWVKNNAAWW
                     ASGQIEDNDFVKGIQYLITQGIMKV"
     gene            complement(2966..>3438)
                     /locus_tag="VLB45_03920"
     CDS             complement(2966..>3438)
                     /locus_tag="VLB45_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PhoU domain-containing protein"
                     /protein_id="HSD04883.1"
                     /translation="SALYDQVGDLTFQMILKYQPVARDFRLIRSSIEISYAFTRFGRY
                     AFDITAVRDMFGDLSECKNDVIYTISGEIRNMITLAIKCFATLDLEKAKQLKTDEDLV
                     DKYYKRHLPMLINSNNTKCARADALVLRYLERIADHAAFMAESVNYIVTGNKKS"
BASE COUNT         1027 a          677 c          594 g         1140 t
ORIGIN      
        1 gttctcaaac agcatcatta caaaggaaat tatgatgaca taatctccga aatggtaaat
       61 tctgtaggtc tcgatatttc ggttctcaaa aaataccctc atgaactcag tggtggaatg
      121 aaacagagaa tagttattgc aatggcactt ttacttaaac caaaatttgt catagcagat
      181 gaacctacta cagcccttga tgtgcttgta caggcacaga taatcaactt gcttaaaaaa
      241 ttaaaaaagg atggcatgtc catcatgctg attacacatg atcttgccat aatatctgaa
      301 attgcagaaa aaattggcat aatgtatgcg ggcaagctag ttgagtttgg aaatgcgtct
      361 gagatttaca aaaatccaaa acatccttac acccaggcac ttttagaatc catcccctca
      421 ttaaaaatca aaaaagagat aaaatacatc aaaggaaacc caccaaatct gctaaaccca
      481 ccagaaggat gcagattcta tgatagatgc ccgcatgcaa tggaaaaatg caaaaaagat
      541 cctccaagat tcaaaacaaa ttcggggtat gttcattgtt ggctatatga ataaaaaata
      601 aaaaaaggaa ttatttctac tgagcgtttc tttgtgcttc aagctctgca cgttcttgca
      661 tttttgcatt gacagatatc tggtcataga ccggattgcc atagtttact cccctgcact
      721 caaatgtaaa agagtcaaga tacacaaagt cagtctttgc attgtatgtc tcctctttgt
      781 caaagtcagt gtagatatag taatcagtca cctgacagtc agtatagaac agctgcctaa
      841 atgcagcgcc tcctcgctgg aacgttacat ccacgtcaaa ttcgctatag tcatgcggga
      901 atgtctttcc ccatttgtgg gattcgtcaa ctgcgtgata cagatatgga gtgtcccatc
      961 ccactacctt ttctaagaca aactttggta cggattttag tgtgtcaaat ccggacgttg
     1021 tcactgtagt tgaggtcaca gtagcagtct gtgagaatgg cttgaagttt ttgaatgtgt
     1081 ttacatcttc ggttgcttca cgaaaatgaa atgccacgcg caatccaact tcatttacgc
     1141 tgtcagtgtc aagcactggt tcttcgtcag caaatgtctt tgttgcatat cctgtaggaa
     1201 tccacaggac tgatgcaatc attgcaattg caacaaatct ttgaagagtt gatttcttca
     1261 tgtgacaatt ttattttgta attacttaaa caatttcaat attgaacatg tgatcaatat
     1321 tggactatga tttactatta aactatatag atataaaaaa taaatttcgt tttatgattt
     1381 ttaactgttc tgttagattt agattatact ttcatgattc cttgtgttat caagtactga
     1441 attcctttca caaagtcatt gtcttctatt tggccacttg cccaccacgc agcattattt
     1501 ttcacccatg atgggatctg ttgcgatgag gtagcagtac cagtttttgt ttcaggtatt
     1561 atcattattt tttctttaac caaatactgg attcccatca cgaaatctcc gtcagatatt
     1621 tgattttctg cccaccactt tgcattattc tttatccaag gcgggatatt gtaagttgat
     1681 tcagatgtag gttttgtact aactattccc gaaccatcat atgcaaacag ctgctgccct
     1741 tcaaatttta ttttagctag tccttgtttt cctagttctc tgacttcatc aggtaatgga
     1801 acatacagta atgacggagc atgctcttga ccgtctgtta tcatccaatg aataagatgt
     1861 actagtgctt ttgcatgttc catatccata ccctttattt tgccaagatc ttgatagacc
     1921 aaaagatacg tgaaacttga gataggatac gagtttttac caggttggtt tactatagac
     1981 actttgctcc aatctccgtc agattttggt aatttagtta cggatgctgc tgcagcagcc
     2041 tttgtagatt caagggatgg ttctacaaag ttagtcttgt cagcattttg gacaaaggca
     2101 aatgtcattt cagattgaac tgcatatgcc aattctacat atcctataga gtacggggtc
     2161 tttcgtacca cgccagcaac accttcattt ccgggagatc ctactccaac tggccatcct
     2221 atactttttc cctttccaat gtttgtatcc catgttttgc ttactgtcga caaataatct
     2281 gtaaatacaa atgtagttcc agatccatca gaacgtctaa ctacaagaat gtctttgttt
     2341 ggaagtacaa catccttgtt tagatcctga atttttttgc cattccattt agttatttta
     2401 cccatgtaga tatcggcaat tacatcaccg gtaagcttga gacctgattt tgcatattct
     2461 ggaatattgt atgaaattgt aactgcacct attgattcag gtatgtgtag agttcctact
     2521 gctattccag attctgctgg tgtcaatggc gcatcagatg ctccaaagtc tacagttttt
     2581 tcagtatgtt gctttacgcc accaccgctt ccaatagatt gatagttgag agttataccg
     2641 gaatagagtt cgttgtattc cactctccat ttatcaatta gaggaaaagg aaatgttgca
     2701 cctgcaccag taatctgaaa ttgacctttt ggctctgcgg gtttcagtac ggcattcgca
     2761 tcaagtgacg tcgtgggcac tattagaacc aatattagta tcaaacctaa aagtatcttc
     2821 tttattttca ttgatcacat aacaatttat tttctattta caattacctc gcatagatca
     2881 cgtaattatc cataatctac tgtaactata tagatttgaa atatttagag tgcaagtaaa
     2941 acttgcacag taaatcaggc ataatttatg attttttatt tcctgtaact atatagttga
     3001 ctgattcggc cataaatgca gcatgatccg caattctttc caaatatctt agaaccagtg
     3061 catcagcacg tgcacatttt gtattattgg aattgataag cattggcaga tgtcgcttat
     3121 aatatttatc aacaagatct tcatcagttt ttagttgttt tgctttttca agatccaaag
     3181 ttgcaaagca ctttattgca agagtgatca tgtttcttat ttctccagaa atagtataga
     3241 ttacatcgtt tttgcattct gaaagatctc caaacatatc acgtacagct gtaatatcaa
     3301 aagcatatct tccaaatcta gtaaaagcat acgatatctc gatggatgat ctgatcaatc
     3361 taaagtctct tgcaactggt tggtatttta aaatcatttg gaaagtcaga tcacctacct
     3421 gatcatataa agcagata
//
LOCUS       DATIDL010000089        12192 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrosopumilaceae archaeon isolate SMAG_U16342
            k141_1321083, whole genome shotgun sequence.
ACCESSION   DATIDL010000089 DATIDL010000000
VERSION     DATIDL010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935836
            Sequence Read Archive: SRR25158326
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosopumilaceae archaeon (soil metagenome)
  ORGANISM  Nitrosopumilaceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrosopumilales;
            Nitrosopumilaceae.
REFERENCE   1  (bases 1 to 12192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/04/2024 05:09:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,520
            CDSs (total)                 :: 1,474
            Genes (coding)               :: 1,474
            CDSs (with protein)          :: 1,474
            Genes (RNA)                  :: 46
            rRNAs                        :: 1 (5S)
            complete rRNAs               :: 1 (5S)
            tRNAs                        :: 43
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12192
                     /organism="Nitrosopumilaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1321083"
                     /isolate="SMAG_U16342"
                     /isolation_source="soil"
                     /db_xref="taxon:2202732"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..709
                     /locus_tag="VLB45_03925"
     CDS             <1..709
                     /locus_tag="VLB45_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007401721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HSD04884.1"
                     /translation="KDFTVEKVSRIGLKINGKSIYPALNDIAIFPSKSAMLMEHVLRI
                     NDEEVWHDSSDGVIISTPIGSSAYSMSAGGPMIFQDSKVFGIVSVNSLDITRRPLIVS
                     EDSFIEFDNISSRLHCEVILDGKDRFKVEKLVTCTKYPQPAHIIRMKKDSTAVSALTK
                     KVKLAEDLLNMPPSSKLLLKTLEYEGSLTQKELSEKTLLPDRTVRLALRHLLEKGYVK
                     RRVSLRDTRQRIYEIPK"
     gene            complement(710..2311)
                     /locus_tag="VLB45_03930"
     CDS             complement(710..2311)
                     /locus_tag="VLB45_03930"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HSD04885.1"
                     /translation="MQTKYIFVTGGVMSGLGKGVVSSSIAKLLQLSNQKVSCVKIDPY
                     LNYDAGTMNPIAHGEVFVTEDGGECDMDIGNYERFLNQNILKSHNITTAQIYSSVIEA
                     ERKGEYLGACVQIIPHVTDAIKNRIRNIAQKESLEMLVVECGGTVGDIESLPFLEALR
                     QMRLEDGPENVIFVHVTLAPSLDVVGEQKTKPTQHSVQELRRIGIQPDIMAVRCTRPL
                     EEAAKKKISLFSNIATRDVFSCHDADSILMVPQMLYDQGIVDVIFKKFSKVGLVNASA
                     NWDKWNAIVDSFANTKETVRIAMVGKYVTLADSYVSVNHALKHAGAKIGKNIMIDWID
                     SEELNGNINKLSSYQGILVPGGFGERGSEGIINTANYAREKNIPYLGICFGFQLAAVA
                     FGRHVCNLSNANSTEISPNTENPVIDLLPEQKSISNLGGSLRLGANEIKVEDNTLAFK
                     LYKSNTITRRHRHRYEFNQKYLESFKQNGMIFSGHSDNKKRMEILEIPTHRFYLGVQF
                     HPEFNSRPGFPEISFESFVTAASQA"
     gene            complement(2343..3149)
                     /gene="trpA"
                     /locus_tag="VLB45_03935"
     CDS             complement(2343..3149)
                     /gene="trpA"
                     /locus_tag="VLB45_03935"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HSD04886.1"
                     /translation="MSKIKNKFLELRSKNQKALIAYVVAGYPNESGTLDAVRGLIKGG
                     ADIIELGLPFSDPLADGPVIQNASHLALKKGINLAKFIGLVKKIRKETELPLVLMTYS
                     NLLYRQGYEKFIALAKKAGIDGFILPDMPLEEAKEYLKAIHKNKADAIFLASPNTTTE
                     RIRKIIKASSGFLYLVSVYGTTGKQQKIQQYTIDAIKNAKKVAGKKIPIGVGFGVSNQ
                     KQAKFIIKCGADAVIVGSAFLRLIDSVPQKKIESSVASFTRNLKAATKSQ"
     gene            complement(3130..4326)
                     /gene="trpB"
                     /locus_tag="VLB45_03940"
     CDS             complement(3130..4326)
                     /gene="trpB"
                     /locus_tag="VLB45_03940"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HSD04887.1"
                     /translation="MRFPKDGKFGEFGGKYIPETLVPAVQELEEAYKKFKDDKNFKKE
                     LDYYLREYAGRPTPLYFAKNLTEAVGGAKIYLKREDLLHGGAHKINNTLGQALLAKKM
                     KKKRIIAETGAGQHGVATAMACACLGLKSEVYMGYKDTQRQKLNVFRMKLLGSAVHPV
                     MEGTQTLKDAINEAIRDWITNVEDTYYLLGSAVGPHPYPVMVRDFQSVIGDEIIKQMK
                     EIEKKTPDSLVACVGGGSNAIGTFYPLLDTDAEMFGIEAGGEGLETKHHSATLNAGSK
                     GILHGMMTYLLQDKEGQILDTHSIAAGLDYPGVGPEHSYLKDSKRVKYDSVADSSAIN
                     AFLMLSKHEGIIPALESSHAVAYAMKIAKSRPKNDTIVVTLSGRGDKDVEVVENYLKE
                     NVKNKK"
     gene            complement(4329..5102)
                     /locus_tag="VLB45_03945"
     CDS             complement(4329..5102)
                     /locus_tag="VLB45_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol-phosphate synthase"
                     /protein_id="HSD04888.1"
                     /translation="MKGMLEKLVQNSQTAINDGVYEISQKISKSKISIIDSIRKNKHA
                     SLITEVKFSSPSLGNIRKISDPVKIARDMIDGGAIGLSVLTQPYLFNGSPEYFQKIRK
                     SVNVPLLMKDVMIDKTQIDAAKKIGADCILLIQAIFDKKLVEDIDEFISYAHKEGLLV
                     LLESHTKKEFLNSIKTDADLLGINNRNLDTLEIDLKTTKNILKDRNEKRIILSESGIE
                     SPKDIKFLHECGADAFLVGSSVMKSKDIKGQVRKLVLAI"
     gene            complement(5099..6145)
                     /gene="trpD"
                     /locus_tag="VLB45_03950"
     CDS             complement(5099..6145)
                     /gene="trpD"
                     /locus_tag="VLB45_03950"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HSD04889.1"
                     /translation="MIKEITDKLSLYEDLTYDEMSQSMDEILKGQVPTQEIANFLRNL
                     TDKGESDQELLAMLDKMQEFAIHIFPKRQDRIIDVCGTGGDKMKTFNVSTASAFVIAG
                     AGGSVAKHGNRAVSGICGSADIFEYFGYDLNMTPEKVTEIIEKFGIGFMFAQKFHPAM
                     KNVSEARKLLGTRTAFNLLGPLSNPAGVKNQVVGVFSADFLERVVLILKARGAERVMS
                     VLSLDGLDELSTTSKNQICLLKDGKISLKILNPAELGLSKASLGDIQVSSKEEAIKAF
                     VSVLRGTANKSMLEVTALNAAAGLIISGIANDFADATPIALECMKTGKAYDVFKNLIR
                     FCGNISKVEEFEKS"
     gene            complement(6142..6729)
                     /locus_tag="VLB45_03955"
     CDS             complement(6142..6729)
                     /locus_tag="VLB45_03955"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HSD04890.1"
                     /translation="MKFLIIDNYDSFVYNIAQILGELGVESDVIRNDKITIDQIKEKN
                     YDAIIISPGPGTPEDEKYFGVCTKVIKELGPTKPILGVCLGHQGIIHAFGGKVVNAGH
                     VRHGKTSPVQYSKTPLFEGVQNPFKATRYHSLVGDKTIIPDELNVTAVALDDKEIMAV
                     SHKKYLIEGVQFHPESIMTSEGKKILSNFLKMVKK"
     gene            complement(6726..8060)
                     /locus_tag="VLB45_03960"
     CDS             complement(6726..8060)
                     /locus_tag="VLB45_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010191410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HSD04891.1"
                     /translation="MLKVIPLEYTGSQFELYNKISRNYTHSFLFESLTGPEELAETSI
                     MGFDPDMVVKCYANRVVLRDKNGNTKTISTNDPLSEISNLLSSVTDQKYRYLGGAVGV
                     INYDAIRLWEKIPDAHKKEETVLMEFGIYNDGILYDNKEKKSFYFYYDKNRINKVPTT
                     DPKFEKFTVSEISENLGKQQFTKMVESAKKYIHDGDIFQVVLSRRFNFEASGDLLKVY
                     KSLRQINPSPYMYHMKMDKKTIIGSSPEMLLRVTGSIVETFPIAGTRAITGDESKNEK
                     MKQEMLSDEKELAEHTMLVDLGRNDIGRVCKYGSVHVKELMAVKKFSHVQHMVTHVAG
                     TLDKKFDTFDAIKAVFPAGTVSGAPKIRAMEIIDELEPESRGPYAGAVGYFSFNGCCD
                     FAIAIRTIFFDGTKGFVQTGAGIVSDSVAENEWMETQHKANAMLDALKEAAK"
     gene            8200..8412
                     /locus_tag="VLB45_03965"
     CDS             8200..8412
                     /locus_tag="VLB45_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007550647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04892.1"
                     /translation="MSQVLVSNQKRNESLLFQDLYFVRKPKFVKSSVTEAVCEICKKG
                     LEDGLSVTALSSEHKTKLYCNNHFPK"
     gene            complement(8419..10095)
                     /gene="ilvD"
                     /locus_tag="VLB45_03970"
     CDS             complement(8419..10095)
                     /gene="ilvD"
                     /locus_tag="VLB45_03970"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007401679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HSD04893.1"
                     /translation="MEISSRNVVEGPSRAPHRAMYKAMGLNDNDLGKPFIGVSHTGNE
                     ATPCNIHLGKIALNAKQGVSDAGGTPREFATIAVSDGIAMGHEGMKASLVSREIIADS
                     IEIMIRAHQYDGLVGLAGCDKSLPGTMLAMARLNLPSVFVYGGTIMPGIYEGKQVTVQ
                     DVYEAVGAYDAGQLTLEALKNLENVACPNAGSCGGMYTANTMASISEAIGIALPGSAS
                     PPAEDPRRAEMCYNSGKAVMNLLENGIRPKDIFTFEAFENAITVANAIGGSTNAVLHL
                     LALSKEIGVHLALEDFERIRKKTPHIADMRPGGFYVMLDLDKIGGIPLLMKSLTKKNL
                     LNLDVMTVTGKTMKKNLESITLSFDSSQKVLKSIESPIHEVGTLTILRGTLAPDGAVV
                     KVAGVKTSEFKGKAKVFDREEDAFDAVAKREIVEGDVVIIRYEGPKGGPGMREMLGVT
                     AALVGQKLGEKVALITDGRFSGATRGLMIGHVAPEAYVGGNIALVKNGDEIRINVEKN
                     TLDLMISESEMEQRRKAWKPRKPNYETGALAKYASLVGSAAEGAITKPKW"
     gene            10195..11118
                     /locus_tag="VLB45_03975"
     CDS             10195..11118
                     /locus_tag="VLB45_03975"
                     /inference="COORDINATES: protein motif:HMM:NF021378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HSD04894.1"
                     /translation="MEAKRLLATVGFFVIAFSLVMPAYADVVTFKTDKTFYGKTGSII
                     FSGTVEEDDYGELVTIVIEDPIGDFAKLISVFPDVDNKFEKSVKIEDWYTTAGIYNAT
                     AFIVNKTSGIVINFDIEKQRPVVTPEPEPEPEPVPEQEDSTPVSEPEPDTETQEDEKT
                     IQEKIQERIEAAKKLKESQTGTPVLGGNSTNTGSTNDGTPSEDNSNDTKTSTGNPIGG
                     IDLNSNLMYIIAGVGGAGAAGAAVYVMRNNYRHKPDYSSYFTQPEKTEPVKPTTQSED
                     DYAMMILKNRLAKGEITVDEFNERKKALKES"
     gene            complement(11120..11722)
                     /locus_tag="VLB45_03980"
     CDS             complement(11120..11722)
                     /locus_tag="VLB45_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide isomerase"
                     /protein_id="HSD04895.1"
                     /translation="MGKKIREEREQKRENYAEKISREKRKYKLVAIGIAAGVAIVLGI
                     TIYMFIQMNNVSPDAPPGAGAIGSTHEHAALKTIIFKDEFDFSKQQFQVKNRFIHFEN
                     HNGETVHLHATGVPMSHLFETIGVGLDDNCFIFPAPDSREFCTNQDYSLKFYINHEKV
                     PSILDYVLKDDDRILISYGNENSTEIDSQLAKVDAVTIDR"
     gene            11956..12111
                     /locus_tag="VLB45_03985"
     CDS             11956..12111
                     /locus_tag="VLB45_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014966009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04896.1"
                     /translation="MPKKREIPIEIDDHFRMYGKEPWEVDYGEKCPLCKARIDEYGFC
                     SCGSGSE"
BASE COUNT         3317 a         2491 c         2187 g         4197 t
ORIGIN      
        1 taaggatttt acagtagaaa aagtttcacg tattggtctt aaaatcaatg ggaaaagcat
       61 ctacccggca ctaaatgata tcgccatatt tccatcaaaa agtgcaatgc tgatggaaca
      121 tgtattgaga attaatgacg aagaagtttg gcatgacagc agtgatggag tcataatctc
      181 tacaccaatt ggctcttctg catattcaat gtcagctggg ggtccaatga tatttcagga
      241 ctccaaagtt tttggaattg tatctgtaaa ctcacttgat attacaagga ggccattgat
      301 tgtatcagaa gacagcttta tcgagtttga taacatttca tcaagacttc actgtgaagt
      361 aatattggat ggcaaagacc gctttaaagt agaaaagctc gttacttgta caaaatatcc
      421 tcagcctgct catataatca gaatgaaaaa agattctact gcagtctctg cgttaacaaa
      481 aaaagtaaag ctggcagagg accttttgaa catgccacca agctccaaat tgttacttaa
      541 aacattagag tatgaaggct cgcttacgca aaaagaactc tctgaaaaaa cccttttgcc
      601 agacagaact gtgaggctgg cattacgcca tttgctagaa aaggggtatg taaaaagacg
      661 cgtctcgtta cgagatacta ggcaaagaat ctacgagatt ccaaaatagc tatgcctgtg
      721 acgctgcagt tacaaaagac tcgaatgata tttccggaaa tccaggcctg ctgttgaatt
      781 ctggatggaa ttgtacccct aggtagaacc tatgggttgg aatctccagt atctccattc
      841 tttttttgtt gtcactgtgt cctgaaaaaa tcattccgtt ctgtttgaac gattcaagat
      901 atttctgatt gaattcgtat ctgtgtctgt gtcttcttgt gattgtattt gatttgtaaa
      961 gtttgaatgc caaagtattg tcttctactt tgatttcatt tgctcctagt cttagtgatc
     1021 ctcctaaatt agaaattgat ttttgttctg gcaagagatc aatgactgga ttttctgtgt
     1081 ttggagaaat ctcagtagaa tttgcattag ataaattaca tacatgtctt ccaaatgcaa
     1141 cagctgctaa ttgaaatcca aaacatattc caaggtatgg aatatttttc tctcttgcat
     1201 agtttgctgt gtttatgatt ccttctgaac ctcgctcgcc aaaaccgcct ggaactagaa
     1261 ttccttggta tgaggaaagt ttgttgatat tgccgtttaa ctcttcagag tcaatccaat
     1321 caatcataat attttttcca atttttgcgc cagcatgttt gagtgcatgg ttaacactta
     1381 cgtagctatc ggcaagcgtg acatattttc caaccattgc aattctgaca gtttcttttg
     1441 tgtttgcaaa agaatccact atcgcattcc atttgtccca gtttgcagat gcgtttacta
     1501 gtccaacttt ggaaaacttt ttgaagatta catcgacaat gccttggtcg tacagcattt
     1561 gtggcaccat gagaatggaa tctgcatcat ggcatgagaa tacgtctctt gtagctatgt
     1621 tagaaaacag agaaattttc ttttttgcag cttcttctag aggccttgta catcttactg
     1681 ccattatatc tggctgtata ccaattctgc gtagctcctg aacgctatgc tgcgtaggtt
     1741 ttgttttttg ttcgcctaca acatcaagtg atggtgcaag ggttacatgc acaaaaatga
     1801 cattttctgg accatcttca agtctcatct gtcttaatgc ttcaagaaat ggcaagcttt
     1861 caatgtcgcc aacagtacca ccgcattcta ccaccagcat ttctaaggat tctttttgtg
     1921 caatatttct gattctgttt tttattgcat cagtcacatg aggaattatc tgaacacatg
     1981 ctccaagata ctctcctttt ctttcagcct cgattacact agaatagatc tgcgctgttg
     2041 taatgttgtg agactttaga atattctgat ttagaaatct ttcatagttt ccaatatcca
     2101 tatcgcactc tccgccgtct tctgtcacaa agacttcgcc gtgtgctatt ggattcatcg
     2161 tgcctgcatc ataattcaga tatggatcta ttttcacaca agaaactttc tgatttgata
     2221 gctgcagtag ttttgcaatc gaagaggaaa caacgccttt tccaaggcca gacatcacac
     2281 cgcctgtaac aaaaatatat tttgtctgca tatctaggga taacaaatcc tctttttatt
     2341 cgttattgtg attttgttgc agctttgaga tttcttgtaa atgaggcaac acttgactcg
     2401 atcttttttt gaggcacact gtctattaat ctcagaaagg cactgcctac tatcactgca
     2461 tctgcgccgc actttatgat gaacttggct tgtttttggt tacttacgcc aaaccctact
     2521 cctattggaa tttttttgcc agccactttc ttggcatttt tgatcgcatc aatggtgtat
     2581 tgctgtattt tttgttgttt tccagtagta ccatacactg ataccaagta aagaaaccca
     2641 gaacttgctt tgattatctt tctgatcctt tcagtggttg tatttggaga tgcaaggaaa
     2701 atggcatcag ccttgttttt atgaattgct tttaggtact cttttgcttc ttctaaaggc
     2761 atgtctggca gtatgaatcc gtcaatgcca gctttttttg caagagctat gaatttttca
     2821 tatccttgtc tgtaaagcaa atttgaatac gtcatcaaaa caagtggaag ttcagtctct
     2881 tttcgtatct tttttaccag tccaataaat tttgccaagt tgatgccttt ttttaaagca
     2941 agatgacttg cgttttgaat tactggtcca tctgcaagag gatctgaaaa gggtaagcca
     3001 agctcaatta tgtctgctcc tcctttgatt aatccacgta cggcatctag cgttccactt
     3061 tcattaggat atccagctac aacataagct atcagtgctt tttgattttt ggatcttagc
     3121 tccaaaaatt tattttttat ttttgacatt ttccttcaaa taattttcta caacttctac
     3181 gtctttgtct ccgcgtccag acaaagtcac cactatagta tcattttttg gtctgctctt
     3241 tgcaattttc atggcgtatg caactgcatg agatgattct agagcaggta taattccctc
     3301 atgttttgaa agcatcaaga atgcatttat tgcactacta tcagcaacac tgtcgtattt
     3361 tacgcgcttt gaatctttta ggtaggaatg ttctggacca actccaggat aatccaatcc
     3421 cgcagctatg ctatgagtat caagtatctg gccttctttg tcttgcagca agtatgtcat
     3481 catgccatgc aaaattcctt tagagccagc atttagtgtg gcagaatgat gctttgtctc
     3541 aaggccttct cctccagctt caatgccaaa catctccgca tcagtatcaa gtagcggata
     3601 aaatgttcca atcgcattag agccgcctcc aacgcaggca actagagagt ctggcgtttt
     3661 tttctcaatt tctttcatct gttttatgat ttcatctcct attactgatt gaaagtctct
     3721 taccattaca ggatatggat gtggacctac tgcagagcca agtaaatagt atgtatcttc
     3781 tacgtttgtg atccaatctc ttatcgcttc gtttattgca tcttttaatg tctgggttcc
     3841 ttccatcaca ggatgtactg cagagccaag caatttcatt ctaaacacat ttagcttttg
     3901 tctttgtgta tctttgtaac ccatgtaaac ttcagatttt aatccaagac atgcgcaggc
     3961 catggccgtt gcaacaccat gctgtcccgc accggtttct gcaatgattc ttttcttttt
     4021 catttttttt gcaagaagag cttgcccaag tgtgttgtta attttatgag ctcctccatg
     4081 caaaagatct tcgcgtttaa gatagatctt tgcgccgcca actgcttctg tcaagttttt
     4141 tgcaaaataa agtggtgttg gtcttcctgc atattcacgc aagtagtaat ctagttcttt
     4201 tttgaaattt ttatcatcct tgaatttttt atatgcctct tcgagctctt gtactgccgg
     4261 gactagtgtc tctgggatgt actttccacc aaactctcca aattttccat cttttgggaa
     4321 tcgtatcttc atatggctaa caccaatttt ctgacctgtc ccttgatatc tttactcttc
     4381 attacgctac ttccaacaag aaaagcgtct gctccgcact cgtgaaggaa tttgatatct
     4441 tttggcgact ctattccgct ttcagaaagt attatcctct tctcatttct gtctttgagt
     4501 atgttttttg ttgttttgag gtctatttcc aaagtgtcca agttacgatt gtttatgcca
     4561 agcaaatcgg catcagtttt gattgaattg agaaattcct tttttgtatg tgattccagc
     4621 aagacaagca ggccttcttt atgtgcatag gaaataaact cgtcaatatc ctctactagt
     4681 tttttatcaa atatggcttg tataagtaaa atacaatctg caccaatttt ttttgcagca
     4741 tcaatctgag ttttgtctat catgacatct ttcatcaaaa gtggtacatt cactgatttg
     4801 cgaatctttt gaaagtattc tggggaacca ttaaacaagt atggttgtgt aagaacagat
     4861 aatcctatag caccgccgtc aatcatgtct cttgcaattt tgactggatc tgatatctta
     4921 cgaatatttc caagtgatgg agaagaaaac tttacctctg ttatcaacga agcatgtttg
     4981 ttttttcgta tgctgtctat tatgctgatt ttggattttg aaattttttg tgaaatttca
     5041 taaactccat cattgatagc tgtctgtgag ttctgtacca gtttttctag catacctttc
     5101 atgatttttc aaactcctca acttttgaaa tgttgccaca aaaacgaatc agatttttaa
     5161 aaacatcata tgctttacca gttttcatac attctaatgc aattggtgtg gcatctgcaa
     5221 aatcatttgc aattcctgaa attatcaaac cggctgcagc attaagtgca gtgacttcaa
     5281 gcatggattt attggcagtt cctcttagta cagagacaaa tgccttgatt gcctcctctt
     5341 tggaagatac ttggatatct ccaagacttg cttttgatag ccctagttct gcagggttta
     5401 ggattttaag gctaatttta ccgtctttta ggaggcaaat ctggtttttt gaggtggtag
     5461 aaagctcatc caatccatcc agggataaaa ctgacattac gcgttctgca ccacgtgcct
     5521 ttagtatcaa cactactctt tcaagaaagt cagctgaaaa cacgccaacc acctgatttt
     5581 ttactccagc aggattgctt aaaggtccga gtaagttaaa cgcggttctt gtcccaagta
     5641 attttcttgc ctctgaaaca tttttcattg caggatgaaa tttttgtgca aacataaatc
     5701 caattccaaa cttttctatt atctctgtga ctttttctgg tgtcatgtta agatcatatc
     5761 caaaatattc aaaaatgtct gcactaccac aaattcctga tactgccctg tttccgtgtt
     5821 ttgctaccga ccctccggct cctgctatta caaaagcaga tgctgtggac acattgaatg
     5881 tcttcatctt gtctcctcct gtgccacata cgtcaattat tctgtcctgt ctttttggaa
     5941 atatgtgtat ggcaaactcc tgcatcttgt caagcattgc caagagctct tgatctgatt
     6001 cacctttgtc agtcaggtta cgcaaaaaat ttgcaatttc ttgtgtaggt acttgacctt
     6061 ttagaatctc atccatggat tgactcattt catcataagt cagatcttcg tatagtgaaa
     6121 gtttgtctgt tatttctttg atcatttttt caccatcttt aagaaattgc ttaggatctt
     6181 ttttccttca cttgtcatga ttgattcggg gtggaattga actccctcta tcaggtattt
     6241 tttgtggctt actgccatga tctctttatc gtctagtgct actgcagtta cattcaattc
     6301 gtctggaatt atggtcttgt ctcctaccag cgaatggtat cttgtggcct tgaatggatt
     6361 ctgaaccccc tcaaacagtg gggtttttga atactggaca ggacttgtct ttccatgtct
     6421 cacgtggcca gcatttacca ccttgcctcc aaaagcatgt atgatgcctt ggtggccaag
     6481 acaaacccca agaatgggct ttgttgggcc tagttccttg attaccttgg tgcacactcc
     6541 aaagtatttt tcatcttctg gagttccagg tcctggagaa attatgattg catcataatt
     6601 tttttctttt atctgatcaa ttgtgatctt gtcatttctt atcacatctg actctacacc
     6661 aagctctcca agaatctgtg cgatgttgta gacaaacgaa tcataattgt ctattatcaa
     6721 aaatttcatt ttgctgcctc ctttagcgca tcaagcattg cgtttgcttt gtgttgtgtt
     6781 tccatccatt cgttttctgc aacagaatct gatacaattc ctgcaccagt ctgaacaaat
     6841 ccctttgttc catcaaaaaa tattgttctt atggcaatcg caaaatcaca gcacccgtta
     6901 aaagaaaaat agccaactgc acctgcatat gggccacgcg attctggttc tagctcatca
     6961 attatttcca ttgcacggat ttttggagca ccagaaacag ttccggctgg aaagactgcc
     7021 tttattgcat caaacgtatc aaatttcttg tccagtgttc cagctacatg tgttaccatg
     7081 tgttgaacgt ggctgaattt ttttactgcc atcaattctt ttacatgtac cgaaccatat
     7141 ttgcaaactc gaccgatatc atttctccca agatctacta acatggtgtg ttctgcaagt
     7201 tctttttcat cacttaacat ctcttgcttc atcttctcat tttttgactc atcaccagta
     7261 attgcgcgtg ttccagctat tggaaacgtc tcaacaatgc ttcctgtaac tctaagtagc
     7321 atctcaggac tggatcctat tattgtcttt ttgtccattt tcatgtgata catgtatggt
     7381 gatggattga tctgtcttag tgacttgtag actttgagta gatctccgct tgcctcaaag
     7441 ttgaatcttc tggaaagtac tacttggaaa atatctccat catggatgta tttttttgca
     7501 ctttcaacca tttttgtgaa ttgttgcttg ccaagatttt ctgaaatttc tgatacagta
     7561 aatttctcaa attttggatc tgtggttgga actttgttga tcctattttt atcatagtaa
     7621 aaataaaatg attttttttc tttgttatca tacaatatgc catcattgta tatcccaaac
     7681 tccattagca cagtttcttc ttttttgtgt gcatctggaa ttttttccca taatctgatt
     7741 gcatcataat ttattactcc tacagcgcct ccaaggtatc tgtatttttg gtcggtgacg
     7801 ctagatagaa gattacttat ctctgacaat ggatcatttg tggaaatcgt ttttgtgttc
     7861 ccatttttat cacgtaacac taccctgttt gcataacact tgacaaccat atctggatca
     7921 aagcccatga tcgaagtctc tgccaactcc tcgggtccag ttagtgattc aaacaaaaat
     7981 gaatgggtat aatttcttga aattttgtta tagagttcga attgggagcc agtatactct
     8041 aggggtatta ccttaagcat tgaatgtcca aatgtggcca cccatataga acgtcctacg
     8101 ctaactctat ttataattaa gtaagatttt gagcaataag tttttacaaa ttacggtata
     8161 gtacggtacc tttatatgcg atcaagccga tcatacaata tgtcacaagt gctggtttca
     8221 aatcaaaagc gcaacgagtc gctgctcttt caggatctat attttgtaag aaaaccaaaa
     8281 tttgttaaaa gtagcgtgac tgaagcagtt tgcgaaatct gcaaaaaagg actagaggat
     8341 ggtctttctg ttactgcact gtcaagtgag cacaagacaa agttgtactg caacaaccat
     8401 tttccaaaat aaactatttt accactttgg ttttgtaatt gcgccttcag cagcagagcc
     8461 taccaatgat gcatattttg ctagtgcacc ggtttcatag tttggttttc ttggtttcca
     8521 agctttgcgc ctttgctcca tctcgctttc tgaaatcatc aaatccaaag tatttttttc
     8581 aacattgatt ctaatttcat caccgttttt tacaagtgcg atgtttccac caacatatgc
     8641 ttctggtgca acgtgtccta tcattaagcc acgagtggct cctgaaaatc ttccgtctgt
     8701 gataagtgct actttttctc caagcttttg accaactagc gcggcagtta ctccaagcat
     8761 ttctcgcata cctggtccgc cctttggtcc ttcatatctt atgattacaa catctccttc
     8821 cacgatttct ctttttgcta ctgcatcaaa tgcgtcttct tccctgtcaa agaccttggc
     8881 ttttcctttg aactctgaag tttttactcc agctactttt actactgctc catctggtgc
     8941 tagagtgcct ctgagtattg ttaatgtccc cacttcatgt attggtgatt caattgattt
     9001 gagcactttt tggcttgaat caaatgagag cgtaatggat tctaggtttt tcttcatggt
     9061 ttttcctgtt acagtcatga catccaagtt tagaagattt ttctttgtca aacttttcat
     9121 caacaaagga attccaccta ttttatcaag atcaagcatg acgtagaatc ctcccggcct
     9181 catgtcagct atgtgcggag ttttttttct aattcgttca aaatcttcta aagcaagatg
     9241 tacgccaatt tcttttgata aagctaatag gtgtagtact gcattggtag aaccaccaat
     9301 tgcatttgct acggttattg cattttcaaa tgcttcaaag gtaaagatgt cttttgggcg
     9361 tatgccgttt tctaaaaggt tcatcactgc cttgccgctg ttataacaca tttctgctct
     9421 tcttggatct tctgctggtg gagaagcgct tccaggtaac gcaattccta ttgcttcgct
     9481 aattgatgcc attgtgtttg cagtatacat cccaccgcaa gaacctgcat ttggacaggc
     9541 aacattttct agatttttca aagcttccaa agttaattgt cctgcgtcat aagctccaac
     9601 agcttcatag acatcttgta ctgtgacttg tttaccttca taaattcccg gcattattgt
     9661 gccaccgtaa acaaagactg aaggaaggtt tagtcttgcc attgctagca ttgttccagg
     9721 caaactcttg tcacaccctg ccaatccaac cagtccgtcg tactggtgtg ccctgatcat
     9781 tatctcaata gaatctgcaa taatctccct gcttacaaga gatgccttca tgccttcatg
     9841 acccattgca ataccgtcac taactgctat tgtggcaaac tctcgtggtg tgccacctgc
     9901 atcacttact ccttgttttg cgttaagtgc aatttttcca aggtgtatgt tacagggagt
     9961 agcctcgttt ccagtatgtg atactccgat gaatggtttg ccaagatcat tatcatttaa
    10021 ccccatggcc ttgtacatgg ctctgtgcgg cgctcttgac ggtccctcta ctacatttct
    10081 gcttgatatt tccattttag atagcaaaag gtggctagag gcataatttt agctttgcat
    10141 cgaaaagtat caattctacg cgtttttaat aaaatagttc aatgaaatca tatcatggaa
    10201 gcaaaaagac tgctagctac ggtaggtttt tttgtaattg cattttcttt agtgatgcca
    10261 gcgtatgctg acgttgtgac ttttaaaact gataagacat tttatggtaa aacaggctcg
    10321 attatatttt ctggcactgt tgaagaagat gactatggtg agctggtcac aatagtcatc
    10381 gaagatccaa ttggagattt tgctaaacta atatcagtat ttccagatgt tgacaacaag
    10441 tttgaaaaat ctgttaagat tgaagattgg tatactactg ctggaatata caatgcaaca
    10501 gcatttatcg taaataaaac aagcggaata gtgattaatt ttgatatcga aaaacaaaga
    10561 cctgtagtga caccagaacc tgaaccagaa cctgaacctg tgccagaaca agaggattca
    10621 actccagttt ctgaaccgga accagacaca gaaacacaag aagatgaaaa aaccattcaa
    10681 gaaaaaatcc aagaaagaat tgaagctgct aaaaaactaa aggaatcgca aactggtaca
    10741 cctgttttag gaggaaattc gactaacact ggttctacaa atgatggaac accatctgaa
    10801 gataattcta atgatacaaa aacctctact ggaaatccaa taggtggaat tgatctaaac
    10861 tctaatctga tgtatattat agctggagtt ggcggtgcag gagctgcggg tgcagctgta
    10921 tatgtgatga gaaataatta tagacacaag ccagactatt cgtcatactt tacacaacca
    10981 gaaaaaactg aaccggtaaa gccaactacg caatcagaag atgactatgc catgatgatt
    11041 ctaaaaaaca gattggcaaa gggagaaatt actgtagatg agtttaacga acgcaagaaa
    11101 gcactgaaag aatcatagtt taacgatcta tggtgactgc atctactttg gcaagctgac
    11161 tatcaatctc tgtactgttt tcgtttccat atgaaattag aattctgtca tcgtccttta
    11221 acacataatc tagaatgctt ggtacttttt catggttgat gtagaatttt aaagaatagt
    11281 cttgatttgt acaaaactct ctgctgtcag gtgctgggaa aatgaaacaa ttgtcatcca
    11341 atccaacacc aattgtttca aagagatgac tcattggaac gccagttgca tgtaggtgaa
    11401 ctgtctcacc gttatggttc tcaaaatgta tgaatctgtt ttttacttga aattgttgtt
    11461 ttgaaaaatc aaattcgtct ttaaatatga ttgtctttag tgctgcatgt tcatgcgtac
    11521 ttccaattgc ccctgctcca ggtggtgcat ctggtgaaac attgttcatt tgtatgaaca
    11581 tgtaaattgt aatgcctaat acgattgcta ctcctgcggc tattcctatt gcaacaagtt
    11641 tgtatttgcg tttttcacgg ctaattttct cagcatagtt ttcacgtttc tgttctcttt
    11701 cttccctgat ttttttgccc attgttaaga agaatcaaaa ttttacccta attaaactca
    11761 actgcaaaat ttgtcataat aattatgatc tgcaaacttg gcaaccgcaa ttcatcatgc
    11821 cggctttgcc catgcattgg tcatgttttt cttcggagca tttgtggcat aaaatcattt
    11881 gatcttccat tgtgttcttt ttgtgagatc tatagttaat aatgtatgta tacttgtatg
    11941 gtgaaaatat tttagttgcc aaaaaagcga gagattccaa tagaaattga tgatcacttt
    12001 aggatgtacg gaaaagaacc gtgggaagtt gactatgggg aaaagtgtcc gctctgtaaa
    12061 gctagaatag atgaatatgg attttgttca tgcggttctg gaagtgaata aatcagaaac
    12121 taaatttccg ttgatttaaa taaaataaaa atgcgcaaca atccatgaac tgcctcttct
    12181 gtgacatctt gt
//
LOCUS       DATIDL010000090         2355 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrosopumilaceae archaeon isolate SMAG_U16342
            k141_917996, whole genome shotgun sequence.
ACCESSION   DATIDL010000090 DATIDL010000000
VERSION     DATIDL010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935836
            Sequence Read Archive: SRR25158326
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosopumilaceae archaeon (soil metagenome)
  ORGANISM  Nitrosopumilaceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrosopumilales;
            Nitrosopumilaceae.
REFERENCE   1  (bases 1 to 2355)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2355)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/04/2024 05:09:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,520
            CDSs (total)                 :: 1,474
            Genes (coding)               :: 1,474
            CDSs (with protein)          :: 1,474
            Genes (RNA)                  :: 46
            rRNAs                        :: 1 (5S)
            complete rRNAs               :: 1 (5S)
            tRNAs                        :: 43
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2355
                     /organism="Nitrosopumilaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_917996"
                     /isolate="SMAG_U16342"
                     /isolation_source="soil"
                     /db_xref="taxon:2202732"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..552)
                     /locus_tag="VLB45_03990"
     CDS             complement(<1..552)
                     /locus_tag="VLB45_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HSD04897.1"
                     /translation="MLPRLDSIKPARKKLGLTQHKLASLTGVSTSMINQIESGRCQPS
                     YATAKKIFEILGSLEGQSDMKAGDICSKDITKLKTTDSLHDAIKKMQEHSISQIPVFD
                     GNDVVGLISEDGLVKHLIDSDETQRKKIKLSEVMESRPPIVDYGIPVKTLVPLVRFSK
                     CILVSKHSKIIGIITASDTLKMME"
     gene            624..1244
                     /locus_tag="VLB45_03995"
     CDS             624..1244
                     /locus_tag="VLB45_03995"
                     /inference="COORDINATES: protein motif:HMM:NF021996.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DM13 domain-containing protein"
                     /protein_id="HSD04898.1"
                     /translation="MLKGITIAIIIGTAAIGTYYLAGPLFYDRITNEPLPQALSNLQK
                     DLTYDKFVNMVDEQRSALVQKMPQDTIDLVMEEAKKFTVTASETMQDMIAKIAPDADE
                     APKFSKLGTFEGLKGHQAKGKAEVISVGDISFLRFEEFEVTNGPDLHVYMTKNGDVST
                     GIDLGQLKGNKGDQNYALDNINTDVYNTVVIYCQPFHVYFASAKLS"
     gene            complement(1262..>2355)
                     /locus_tag="VLB45_04000"
     CDS             complement(1262..>2355)
                     /locus_tag="VLB45_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007550748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="putative PEP-binding protein"
                     /protein_id="HSD04899.1"
                     /translation="ARSYGAKGIGLCRTERMFNARDRLGVFVEMIMARTIQQRKIILE
                     KLLVLQKSDFKEILQAMEGYHVTIRLLDPPLHEFLPNIEELINRMHRFEKQNVPEEME
                     STKKILDRARELAEINPMMGHRGVRIGITFPEIYEMQIRAICEAAGELSKDKVDARAQ
                     IMVPQVSSISELNQIKQIYDNIKSSAESKLGIKLKINFGTMLEVVRSCLTADELAKTA
                     EFFSFGTNDLTQAVFSFSREDAEGKFLTEYLDKELLAVNPFQSVDVNGVGSLMEIAIS
                     KGRKLRPKMEIGICGEHGGDPNSIRFFHKAGLSYVSASSHRIPIAIIAAAQAALAEKS
                     KKTNVKPKKKTKTRRKKKTRTKHKKKSRR"
BASE COUNT          607 a          485 c          440 g          823 t
ORIGIN      
        1 ctccatcatc ttgagagtat ctgaagcagt aattatccca attatcttgc tgtgctttga
       61 gacaagaatg cattttgaaa atctgactag aggcaccagt gtcttgactg gtatgccata
      121 atccactatg ggcggtcgtg attccatgac ttcggaaagt ttgatctttt tgcgttgtgt
      181 ttcatcacta tctatgagat gctttacaag accatcttcg cttatcagtc ccacaacatc
      241 atttccatca aaaactggaa tctgactaat tgaatgctct tgcatttttt ttattgcatc
      301 atgaagtgaa tctgttgtct ttagttttgt aatgtccttg ctacaaatgt cgcctgcttt
      361 catatctgac tggccttcta gtgacccaag aatttcaaag attttttttg cagtggcata
      421 gcttggctgg catctgcctg actcgatttg gtttatcatt gaagtgctga ctcctgtcag
      481 tgaagcaagc ttgtgttggg taagcccaag tttttttctt gctggtttta tagaatcaag
      541 tcttggtagc acaaaagcaa ttatcttggt ttgctatttc aattttcttc aatccttaat
      601 agtctgattt tacaaattca tgtatgctaa agggtatcac tattgcaata atcatcggca
      661 ctgcagcaat tggaacatac tatcttgcag gtccgctgtt ttatgataga atcactaacg
      721 aaccattgcc gcaagcgcta agcaatttgc aaaaagatct tacatatgac aaatttgtaa
      781 acatggtaga cgagcaacga agtgcgctcg tgcaaaaaat gcctcaagac acaattgatc
      841 ttgtgatgga agaggcaaaa aaattcactg tcacagcttc agaaaccatg caggatatga
      901 tagctaaaat tgcaccagat gcagatgagg cccccaaatt ctctaaactt ggaacctttg
      961 agggactaaa aggccatcaa gctaaaggca aagctgaggt catctcagtt ggtgacattt
     1021 cttttctcag gtttgaggaa tttgaagtta caaatggtcc agaccttcat gtatacatga
     1081 caaaaaatgg cgatgttagc accggaatag atctaggaca actcaaagga aacaaaggag
     1141 accagaacta tgctcttgac aatatcaata ctgatgtgta caatacggtt gtgatttact
     1201 gtcagccgtt tcatgtttac tttgcatctg ccaagctgtc ctaaaatttt tttaatctag
     1261 tttatcttct tgatttcttt ttatgttttg ttctagtttt ctttttgcgt cttgttttag
     1321 ttttcttttt aggtttcaca ttggttttct ttgatttttc tgcaagtgca gcttgtgctg
     1381 ctgcaataat tgcaattgga attctgtgag atgaagcact gacatagctc aggcccgcct
     1441 tgtggaaaaa tctaatcgag ttagggtctc ctccgtgctc tccgcatatt ccaatctcca
     1501 ttttcggtct gagttttctt cctttagata tggcaatttc cattaagctt ccaactccgt
     1561 ttacgtctac tgactggaat ggatttactg caagcagttc cttgtctagg tattctgtaa
     1621 ggaattttcc ttccgcatct tctctgctaa aactaaacac tgcttgtgtg aggtcgtttg
     1681 taccaaagct aaagaactct gcggttttgg caagctcatc agcagtaaga catgatctga
     1741 caacttcaag catggtgcca aagttgatct ttagttttat tcctaatttt gattccgctg
     1801 aagattttat gttgtcatag atttgcttga tttgatttaa ttcgcttatg ctgcttacct
     1861 gtggcaccat tatctgggca cgggcgtcta ccttgtcttt tgaaagctcg cctgcagcct
     1921 cacaaattgc tcttatctgc atttcataaa tttctggaaa tgttatgcct attctaactc
     1981 cacgatgtcc catcattggg ttgatttcag ccagttctct tgcacgatct agaatctttt
     2041 ttgtactttc catctcttct ggaacatttt gtttttcaaa cctgtgcatt ctgttgatca
     2101 actcttcaat gtttggcaag aattcatgca acggcggatc tagcaatctg attgtaacat
     2161 gatatccttc cattgcttgc agtatttctt taaagtcgct tttttgcaaa accagaagtt
     2221 tttctaaaat tatttttctt tgctgaatag tacgtgccat gatcatttct acaaaaactc
     2281 ccaacctgtc acgcgcgtta aacatccttt ctgttctgca aaggcctatg ccttttgcgc
     2341 catagcttct ggcta
//
LOCUS       DATIDL010000091         3268 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrosopumilaceae archaeon isolate SMAG_U16342
            k141_1324185, whole genome shotgun sequence.
ACCESSION   DATIDL010000091 DATIDL010000000
VERSION     DATIDL010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935836
            Sequence Read Archive: SRR25158326
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosopumilaceae archaeon (soil metagenome)
  ORGANISM  Nitrosopumilaceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrosopumilales;
            Nitrosopumilaceae.
REFERENCE   1  (bases 1 to 3268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3268)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/04/2024 05:09:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,520
            CDSs (total)                 :: 1,474
            Genes (coding)               :: 1,474
            CDSs (with protein)          :: 1,474
            Genes (RNA)                  :: 46
            rRNAs                        :: 1 (5S)
            complete rRNAs               :: 1 (5S)
            tRNAs                        :: 43
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3268
                     /organism="Nitrosopumilaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1324185"
                     /isolate="SMAG_U16342"
                     /isolation_source="soil"
                     /db_xref="taxon:2202732"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..437
                     /locus_tag="VLB45_04005"
     CDS             <1..437
                     /locus_tag="VLB45_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008297329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HSD04900.1"
                     /translation="YNGKFEDTNLRWNKGNPDTEIIQRYYSHMDKTGMKPKTKATAIQ
                     IAKPANILKGLRALEFTNGVVTQVSDQEMMDGMSLVGLNGFDCEMASGAVPAGIKKLV
                     EEEIIKKDDTVVGILTGRQKDSSIPVEYHKNPHNKFANPPRT"
     gene            complement(439..1344)
                     /locus_tag="VLB45_04010"
     CDS             complement(439..1344)
                     /locus_tag="VLB45_04010"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HSD04901.1"
                     /translation="MSDTDTWKKTDGSEILRKGQIAAKQSVITLAAIGVAELLVAMFV
                     GSITLTADGLDSLADSMISFIVWFGIMMGKKPRSKLFHYGYKKVEVLAALIAAIIIVI
                     LGGYIAYHAFQALYEPRTILHPEIAMITLIAAGSISLHRAFKIRKVAMESNLISLKLD
                     AKNSIKDGTSSFVGFVSIALATYLNFYFMDAIGGMIIAGYIFVMAYIAIKESALVLVD
                     AVENPQMTENVKNVIYNKFKIKTKDVFLRPIGREFNAEIHIFLPNETRLDETSRIVNE
                     ISKTIKNDLHLARVVIVPEPENSVN"
     gene            complement(1399..2448)
                     /locus_tag="VLB45_04015"
     CDS             complement(1399..2448)
                     /locus_tag="VLB45_04015"
                     /EC_number="1.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HSD04902.1"
                     /translation="MKKVFVNGYGAIGSRITQFIAGDSDIELIGVGKYSPDEKVTDAI
                     SRGFAVYVPKNKVDLFKNFKISGTIEDALEKCDLVIDAAPGGQGYANKKNLYEPKNVR
                     AIFQGGEKLTGEKAVSDFIFNSRVNFNDAFDKKYAMQGSCNVTGMGRILQPLKEKFGS
                     RIKRFDATLLRRWADLEDLKTEVKDSIEWTRKPHHDEDVKSYMGKDTPLFIRVFKVPT
                     RQMHVHLMDIRFNGVAPKSLEILDLYKNEYGVAILYGAKGTKDIRDFAESTKFIFKDT
                     NMIHIHADLLEIQDDIVKLTYSDDQTGIVVPENHLLMQAMLFKRNSDDAFKHTESLFH
                     MEEKKRLLEAHFKQT"
     gene            2525..3253
                     /locus_tag="VLB45_04020"
     CDS             2525..3253
                     /locus_tag="VLB45_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HSD04903.1"
                     /translation="MNIHGPSLGIGAAIAVISILGTLFVTNSDFSNQIIKTQEQEDDK
                     VIKSTQVQKSKDEMALSLFSAQASSILGSSDAPVTIVEFGDYQCFYCNKFFHTTESEI
                     VKNYVDTGKVKMIFKDFTIIGQDSINAANAAHCANEEGKFWEYHDMLYNHWTGENNGW
                     ASLENLAKFADDIGLNKAQFDSCMTEQRYFTIIESSNSDARTLGLTGTPGFFIIGPDN
                     TIAKIGGAQPYEVFEKTIESKLEK"
BASE COUNT         1055 a          618 c          544 g         1051 t
ORIGIN      
        1 tttacaacgg aaagtttgaa gatacaaatc tacgttggaa taaaggcaat ccagatacgg
       61 aaataataca aagatactat tcgcacatgg ataaaactgg gatgaagcca aaaacaaaag
      121 caactgcaat ccaaattgca aaacctgcaa acattctcaa aggattaaga gcattagagt
      181 tcacaaacgg tgtagtcact caggtttcgg atcaggaaat gatggatggc atgtcgctag
      241 tagggttaaa tgggtttgat tgtgaaatgg catcaggtgc agtacctgca ggaattaaaa
      301 aactggttga agaagaaata attaaaaaag atgatacggt ggttggaatc cttacaggaa
      361 ggcaaaagga ttcaagcatt cctgtagaat accataagaa tccacacaac aaatttgcaa
      421 atccgccaag aacataaact agtttacgga attttctggt tcaggaacaa tcacaactcg
      481 tgccagatgc aaatcatttt ttattgtttt tgatatctca ttaacaattc tgctagtctc
      541 gtctaatctt gtttcattag gcaaaaatat gtgaatttca gcgttgaatt ctcgtcctat
      601 tggtcttaag aatacatctt tggttttgat cttaaacttg ttataaatga cattttttac
      661 attttcagtc atttgtggat tttctactgc atctacaagt actaatgccg actctttaat
      721 tgcaatgtat gccataacaa aaatgtaacc tgcaattatc atgccaccaa tagcatccat
      781 aaaatagaaa tttagatatg ttgcaagtgc aatacttacg aatccaacaa atgatgacgt
      841 tccatctttg atcgaatttt ttgcatcaag ttttagtgaa ataaggttag attccattgc
      901 aacttttcta attttaaatg cacgatgtaa agagatgcta ccagctgcaa tgagtgtaat
      961 cattgcgatt tctggatgaa gtatggtacg cggttcatag agtgcttgaa atgcgtgata
     1021 tgctatatag ccaccaagga ttacgatgat gattgcagca attagcgctg ctaaaacctc
     1081 gacttttttg tatccgtagt gaaatagttt gctccttggt tttttcccca tcattattcc
     1141 aaaccacaca ataaaggaga tcatggaatc tgctaacgaa tccaacccgt cagcagtaag
     1201 tgtaatactt cccacaaaca ttgccacaag cagctcagct acgcctatag ctgcaagtgt
     1261 aattactgat tgtttagcgg caatctgtcc tttacgaagg atttcagatc cgtcagtttt
     1321 tttccatgta tctgtatcac tcaatgtaaa atcttatcta atgaaaaatg ccatgtccat
     1381 cttttaaatc atctgtcact aagtttgctt gaaatgcgct tcaagcagtc tctttttttc
     1441 ttccatgtga aataatgatt ccgtatgctt gaatgcatca tctgaatttc ttttaaaaag
     1501 cattgcttgc attaacagat gattttctgg cacaacaatt ccggtttgat catcagagta
     1561 ggttagtttt acaatatcat cctgaatctc tagcaaatcc gcatgtatgt gaatcatgtt
     1621 tgtatcctta aagataaatt ttgtagattc tgcaaaatct cgaatatctt ttgtgccttt
     1681 tgcaccatac aaaattgcaa caccatattc atttttgtat aaatccaaaa tttctaaaga
     1741 tttgggagca acaccattga atcttatatc cataagatgc acatgcattt gtcttgttgg
     1801 gactttgaaa actctaatga atagtggagt atcttttccc atgtaacttt ttacatcttc
     1861 atcatgatgt ggtttccgtg tccattctat ggaatctttt acttctgttt tcaagtcttc
     1921 tagatctgcc caccggcgca aaagcgtagc atcaaatcgt tttattctag aaccaaattt
     1981 ttcttttaat ggttgaagaa ttcttcccat tccagtaaca ttacaacttc cttgcattgc
     2041 gtatttctta tcaaaagcat cattgaagtt tacgcgtgaa ttaaaaataa aatcagatac
     2101 tgccttttcg cctgtaagct tttcaccgcc ctgaaaaatt gctcttacat tttttggttc
     2161 atacaaattc tttttatttg catatccttg accaccaggt gctgcatcta ttactaaatc
     2221 acatttttct aatgcgtctt cgatagtacc agatattttg aagtttttaa ataaatccac
     2281 cttattttta ggaacataaa ctgcaaatcc tcttgaaata gcatcggtaa ctttctcatc
     2341 tggagaatat tttccaacac caattaattc gatgtctgaa tctcctgcga tgaattgtgt
     2401 aattctactt ccaattgcac cataaccatt aacaaaaacc ttcttcatga tttttttata
     2461 attatcctct ttatttagat acttgtgaca agaactaaat atatcaaaaa acctgctgat
     2521 gccattgaac atacatggtc catcccttgg tattggtgca gcaatagcag taatttctat
     2581 ccttggaaca ctttttgtca cgaattcaga tttttccaat caaatcataa aaactcaaga
     2641 acaagaagat gacaaagtca taaaatccac acaggttcaa aaaagcaaag atgagatggc
     2701 cttgtcgctt tttagtgcac aagcatcttc aatacttgga tcctctgatg ctcctgtcac
     2761 aattgtagaa tttggtgact atcaatgctt ttattgtaac aaattctttc acactacaga
     2821 atcagaaatt gtaaaaaact atgttgatac aggaaaggtc aaaatgattt tcaaagattt
     2881 tacaatcatt ggtcaagatt ctataaacgc agcaaatgca gcacattgtg ccaatgagga
     2941 aggaaaattc tgggagtacc acgatatgct ttacaatcac tggaccggtg aaaacaatgg
     3001 atgggcctca cttgagaact tggcaaaatt tgctgatgac ataggattga acaaggcaca
     3061 gtttgattca tgcatgacag aacagcgcta ttttacaatt attgagtcta gcaattctga
     3121 cgctagaacg ctgggactaa ctggtacgcc tgggtttttc atcataggtc ctgataacac
     3181 catagccaag ataggcggcg cacaaccata cgaagtattt gaaaaaacta tagagtcaaa
     3241 gctagaaaaa taaaataatt tcttcaat
//
LOCUS       DATIDL010000092         2631 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrosopumilaceae archaeon isolate SMAG_U16342
            k141_2335387, whole genome shotgun sequence.
ACCESSION   DATIDL010000092 DATIDL010000000
VERSION     DATIDL010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935836
            Sequence Read Archive: SRR25158326
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosopumilaceae archaeon (soil metagenome)
  ORGANISM  Nitrosopumilaceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrosopumilales;
            Nitrosopumilaceae.
REFERENCE   1  (bases 1 to 2631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/04/2024 05:09:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,520
            CDSs (total)                 :: 1,474
            Genes (coding)               :: 1,474
            CDSs (with protein)          :: 1,474
            Genes (RNA)                  :: 46
            rRNAs                        :: 1 (5S)
            complete rRNAs               :: 1 (5S)
            tRNAs                        :: 43
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2631
                     /organism="Nitrosopumilaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2335387"
                     /isolate="SMAG_U16342"
                     /isolation_source="soil"
                     /db_xref="taxon:2202732"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..386)
                     /locus_tag="VLB45_04025"
     CDS             complement(<1..386)
                     /locus_tag="VLB45_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007403146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HSD04904.1"
                     /translation="MELIQIANQKVPTLGKKSTIRFTQSICPDCNMILDAEVFERDGK
                     VFMTKTCPTHGECEELYFGSYEMYKKFSTYWMDGKGAHAPNVMIEKCSCPNNCGLCSN
                     HLSHSGLANMIVTNRCDLTCWYCFFYV"
     gene            535..1305
                     /locus_tag="VLB45_04030"
     CDS             535..1305
                     /locus_tag="VLB45_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HSD04905.1"
                     /translation="MSLSDKTRKALEKVGLTSYEIKTYSALLKTGQINASDLSQKSGV
                     PYSKIYEVLGTLEEKGWIGSDESRPTQYFAKPPTTAIENVRQSVETEFKNNESVILKE
                     LTPLYEQSGTSERPDIWVLSGVVNIASKILEMVDSCRNEVLIAIPKVAEDLAREALPK
                     LRSLHDRGVEITILVSEDVNSETLKSLSRVASVKIKKGLFGGGIISDKRYVVILLGED
                     LGTSRSAEAVAIWADHSGLAGFAREYFEYLLKESKDVK"
     gene            1325..1762
                     /locus_tag="VLB45_04035"
     CDS             1325..1762
                     /locus_tag="VLB45_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HSD04906.1"
                     /translation="MKDEVLFVGTAEAEHVEMYLKAIWHIKESGNPVKISSISKMLNV
                     RQPSVVQMLKKLNEAKLVNYNKTGVSLTKSGEQVGTNMMRNSRLLEVLMDSALKVDID
                     EEMVCGIEHHMNEQFTDALCTMLKHPRKCPHEHDIPRGKCCKN"
     gene            complement(2018..2106)
                     /locus_tag="VLB45_04040"
     tRNA            complement(join(2018..2053,2069..2106))
                     /locus_tag="VLB45_04040"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2070..2072),aa:Lys,seq:ctt)
     gene            complement(2160..>2631)
                     /locus_tag="VLB45_04045"
     CDS             complement(2160..>2631)
                     /locus_tag="VLB45_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008298012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HSD04907.1"
                     /translation="DVKAGMGVGTTFRVIGDFDGKRLEWDCETTEFIQEERLAAKMIK
                     GPFKKWEIAMEFKKLEDKLTRLTMTVNYDMPLGPLGGILNKIKFAKTAEKGMETALFK
                     VRGLLEGNGSIPVYITLDAYRKLLAEKEKMHNAPVSTVLTKILEKYSQIESVKN"
BASE COUNT          848 a          462 c          551 g          770 t
ORIGIN      
        1 acatagaaga agcagtacca gcatgtaaga tcacatctgt ttgtaactat catgtttgcc
       61 aatccactat gggataggtg gtttgaacac agtccacagt tatttgggca tgaacatttt
      121 tcaatcatta catttggagc atgggcgccc tttccgtcca tccagtaagt actgaatttt
      181 ttgtacattt cataagagcc aaagtagagc tcctcgcatt caccatgtgt tggacatgtc
      241 tttgtcatga agactttgcc gtctctttca aagacttcgg catccaaaat catgttacaa
      301 tctggacaaa tactttgagt aaatcgaata gtagactttt tacctaatgt aggtaccttc
      361 tggttggcaa tctggattag ttccatacta aacgcttgtt ttcaaagata gtatataatc
      421 gatttcctac tagacaccag tggttttagg agattgatcg gttacacagg catgagacga
      481 tttgtgaata gccatagttg acatatatat caaaagcgct tcgaatgaga atagatgagt
      541 ctttcagaca agacacgaaa ggcattggaa aaggtgggcc tgacaagtta tgagataaag
      601 acgtattctg cacttttgaa aacaggtcag ataaatgcgt ctgatcttag ccagaaatca
      661 ggagttccat attcaaagat atacgaagtg ctaggtactc tagaagaaaa gggatggatt
      721 ggatctgatg aatcaagacc aacgcagtat tttgcaaagc cgcctacaac tgccatagaa
      781 aacgtaagac agagtgtgga aacagagttc aaaaataatg aatctgtaat tttgaaagaa
      841 ctcactccac tttatgaaca gagtggcacg agtgaacgac ctgatatttg ggttctttct
      901 ggagtagtaa acattgcttc taaaattttg gagatggtag actcgtgtag aaacgaagtt
      961 ctgatagcta taccaaaagt agcagaggat cttgcaagag aagcattacc caagcttaga
     1021 tcattacatg acaggggagt tgaaatcacc atactggttt ctgaggatgt gaattcagaa
     1081 actctaaaat cattatcaag agtggccagt gtaaaaataa agaaggggct ttttggaggt
     1141 ggcataatat cagataagag gtatgttgta atactgcttg gcgaagatct tggcacatcc
     1201 agatctgcag aggcagtagc aatatgggca gatcattctg gtcttgcagg ttttgctcgc
     1261 gaatattttg aatatttatt aaaagaatca aaggatgtaa agtaggtaaa aaggaaaatc
     1321 taacatgaaa gatgaagttt tgtttgttgg tactgccgaa gcagaacatg tggagatgta
     1381 tcttaaagca atttggcaca taaaagaaag cggaaacccg gtaaagatta gttcaatatc
     1441 aaagatgcta aatgtgagac agccaagcgt tgtccagatg ttgaaaaagc taaatgaggc
     1501 aaagcttgtc aactataaca aaactggagt atctttgact aaaagtggag aacaggtagg
     1561 caccaacatg atgcgaaaca gcagactgtt agaagttcta atggatagtg cactcaaagt
     1621 tgacattgat gaagaaatgg tatgcggaat agagcatcac atgaatgaac agtttactga
     1681 tgcactctgt acaatgctaa agcacccaag aaagtgtcca catgaacatg acatcccacg
     1741 tgggaaatgt tgcaaaaatt gattttgaaa taagttatat cagagtagag aatttgaaac
     1801 aatatggttt gtttttgtgg ttgcgaaaca tatgttcggg atgaaaatgg tttcaaagta
     1861 ggttgtgtaa agtgcagcca cggtccacag aatcatgaag aaagcttcaa gctgaaatca
     1921 aatcttgaag acagccaaaa gcgcggttca ttgtcataat tattttctaa atttcgtaaa
     1981 tcattttcaa aaaacaaaac atgtcaaaac ataaaggcgg gtctagtggg atttgaaccc
     2041 acggcatagg gatttctctg aaagatcata agagtccctc gctctacctg gctgagccat
     2101 agaccccgca aaatagcaga cgccaatttc gttatttaaa ctagagcgga aataaagtac
     2161 taatttttga cagattcgat ttgtgagtat ttttccagta tttttgtcaa tactgtagaa
     2221 actggagcat tgtgcatttt ttctttttct gccaacagct ttctgtatgc atcaagagtt
     2281 atgtaaactg gaattgagcc gtttccttct aacaatcctc gcactttgaa gagtgcggtt
     2341 tccattcctt tttcagcagt ctttgcaaat ttaatcttgt tgagaattcc acctaatggt
     2401 ccaagtggca tgtcatagtt tactgtcatt gtaagccttg tcagcttgtc ttctaatttt
     2461 ttaaattcca ttgcaatttc ccattttttg aatgggcctt tgatcatctt tgctgccagt
     2521 ctttcttctt gaataaattc tgtagtctca caatcccatt caagtctttt gccatcaaag
     2581 tctcctatta cacgaaatgt ggtaccaaca cccatgcctg ctttaacatc c
//
LOCUS       DATIDL010000093         4754 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrosopumilaceae archaeon isolate SMAG_U16342
            k141_2537295, whole genome shotgun sequence.
ACCESSION   DATIDL010000093 DATIDL010000000
VERSION     DATIDL010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935836
            Sequence Read Archive: SRR25158326
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosopumilaceae archaeon (soil metagenome)
  ORGANISM  Nitrosopumilaceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrosopumilales;
            Nitrosopumilaceae.
REFERENCE   1  (bases 1 to 4754)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4754)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/04/2024 05:09:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,520
            CDSs (total)                 :: 1,474
            Genes (coding)               :: 1,474
            CDSs (with protein)          :: 1,474
            Genes (RNA)                  :: 46
            rRNAs                        :: 1 (5S)
            complete rRNAs               :: 1 (5S)
            tRNAs                        :: 43
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4754
                     /organism="Nitrosopumilaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2537295"
                     /isolate="SMAG_U16342"
                     /isolation_source="soil"
                     /db_xref="taxon:2202732"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1388
                     /locus_tag="VLB45_04050"
     CDS             <1..1388
                     /locus_tag="VLB45_04050"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HSD04908.1"
                     /translation="ITSEILKKPQEKKSLFGMRKITKISYVIVVVALLLIPAIFPVNA
                     NWISITKAPPTILNGGSNFVMATNDWPATLEWIKNNTPKDAVIASWWDYGYWITTIGE
                     RKSIADNATLDTSTIAKLAKMLLSSPDDAWKILQELDADYVLVYVVGQKFVADDQELY
                     VLGGGGDESKKQWFARIGGEDLSRLVQNDGFTPTNYFWDNTLLGKMFPFSPVAYYNFN
                     TNQQIENYQTGYTGIYLKTIKYETDVDPIKLVYSSPSLDRKNAGPVSGVLVYQINHNY
                     VVSDTKPSDLSAESESTPTGNVAVLSTNFGDIVIDFKEDVAPQTVANFEKLANSGFYD
                     GTLFHRIMPGFVIQGGDPNTISGSRDTWGTGDPGYKIPPEFSALKHKKYIVSMARGSD
                     INSAGSQFFIMLGDAPWLDNAYTIFGEVISGQDAVDKIALLETNSENQPIDIESARIK
                     QVIIRSQEPET"
     gene            complement(1414..1569)
                     /locus_tag="VLB45_04055"
     CDS             complement(1414..1569)
                     /locus_tag="VLB45_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-protein complex protein Nop10"
                     /protein_id="HSD04909.1"
                     /translation="MRFQMRKCPDCKRYTLKDECPMCGNKTVTVHPGKYSPDDKYARY
                     RISDRYK"
     gene            complement(1571..2371)
                     /locus_tag="VLB45_04060"
     CDS             complement(1571..2371)
                     /locus_tag="VLB45_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007403187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 subunit
                     alpha"
                     /protein_id="HSD04910.1"
                     /translation="MTTEIQELPEVGEIVVATVTRIMDQGAYVSLDEYNNIQGFLHIS
                     EIATGWIRHVEKFVKVGEKKVLLVKRVNTARSEIDLSLKQVSSDQKKKKLLELKRNEK
                     EQILIDSLRTKAGLTQAEAEKLEGVLVNKFNSIYDAFSEIVSKGITVLDDLNLPKKTL
                     STIEEISSKIQVPHVEIRGVLELTSTKSNGIEIIKKSLLEAMESKDASVEITYIGAPK
                     YRLTVSAQNFKTAEKALKPVLLTIQTTIEKKGGTYKFTREESKKTREG"
     gene            complement(2410..2943)
                     /gene="cobO"
                     /locus_tag="VLB45_04065"
     CDS             complement(2410..2943)
                     /gene="cobO"
                     /locus_tag="VLB45_04065"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HSD04911.1"
                     /translation="MGKDGLVIVYTGKGKGKTTAALGMALRAVGYNYKICMIQFIKGT
                     WHYGEMTSSKRLEPEFELTAIGKGFVGIIDDKSPHAEHEKIAIEALRLSREKIESEKY
                     DIMILDEINYAVNLGLVKIEDVLDLIKIKPANLNLVLTGNHAKQEIIDAADLVTEMKE
                     IKHPFKSGIKAKKGIDF"
     gene            3087..3212
                     /locus_tag="VLB45_04070"
     CDS             3087..3212
                     /locus_tag="VLB45_04070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04912.1"
                     /translation="MAEAGLGYFGAMIGVMIAMAVVCFSLRGKRPHGPSVEQSGT"
     gene            3321..4547
                     /locus_tag="VLB45_04075"
     CDS             3321..4547
                     /locus_tag="VLB45_04075"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010195064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HSD04913.1"
                     /translation="MTREYTYDVVIVGAGPAGSSAAWAAAKKGVRVALLEREEAIAQS
                     IRTSGVTWINDMKKFNITSDYYNPIKNYSFHSLNNHVTVHGSEYQAAVLDVRRTYQFL
                     ACEAANAGADIFVRTNATNPLIDSKGNLVGIKATSLKDDLIFHCKLVIDASGFYSVIG
                     KSIGIVPQWKRFGVGAEYEAYVEKVDPETWSLMVGQQYSPAGYAWIFPLGKNKVRIGV
                     GIGKPESKIDPTQRLLELVEKKPGPLGQLGKITPIEFHYGLIPNEGLTHTTIYNNIIL
                     VGDAAGQANPLVLEGIRYAIEFGRIAGQVGAESVISGDTSKTSLKPYEDAWKKAISSK
                     IKAAIKVQNRWLNLTDEEWDKELEIIKELSPDEFLDFIKADFSISSMIGLATHHPKLA
                     VRQLFDIVKSISGTHQ"
     gene            4601..>4754
                     /locus_tag="VLB45_04080"
     CDS             4601..>4754
                     /locus_tag="VLB45_04080"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobyrinic acid a,c-diamide synthase"
                     /protein_id="HSD04914.1"
                     /translation="MNISRIVLAGTTSGVGKTSITAAIIHGIKKRGYSVQSFKVGPDF
                     IDPSYLS"
BASE COUNT         1520 a          838 c          847 g         1549 t
ORIGIN      
        1 taattacctc tgaaatatta aaaaaaccac aagaaaaaaa atctcttttt ggcatgagaa
       61 aaatcacaaa aatatcttat gttatagttg tagttgcatt actcttaatt cctgccattt
      121 ttccagttaa cgctaactgg atatcaataa ccaaagctcc tcctacaatt cttaacggcg
      181 gttctaattt tgttatggct acaaatgact ggcctgcaac tctagaatgg ataaaaaata
      241 atactccaaa agatgctgtg attgcttcat ggtgggatta tggatactgg ataaccacaa
      301 tcggtgaacg aaaatctatt gcagacaatg caactttgga cacaagtaca atagctaagc
      361 tagcaaaaat gttacttagt tcccccgatg atgcatggaa aatattacaa gaattagatg
      421 ctgattatgt tttggtatat gtagttggac aaaaatttgt tgcagatgat caagaacttt
      481 atgtccttgg cgggggtggc gacgaaagca aaaaacagtg gtttgctcgc atcggaggcg
      541 aagatctatc tagattagtt caaaacgatg gatttactcc aaccaattat ttctgggata
      601 atactctttt aggaaaaatg tttccatttt cacctgtagc atattataac ttcaacacaa
      661 atcaacaaat agaaaattat caaactggat atactggaat atatctcaaa acaataaaat
      721 atgaaactga tgttgatcca ataaaactag tttattcatc accaagtctt gatagaaaaa
      781 atgctggtcc agtttcaggc gtattggtat atcagatcaa ccataattat gttgtatctg
      841 acacgaaacc gtctgatctc agtgcagaat ctgagagcac tccaactggt aatgttgctg
      901 tattgtcgac taattttggt gatatagtaa ttgatttcaa agaagatgtt gcacctcaaa
      961 ctgtagcaaa ctttgagaaa cttgcaaatt caggattcta tgatggaact ctatttcaca
     1021 gaataatgcc tggctttgtt attcaaggag gagatccaaa tacaatttca ggatcgcgtg
     1081 atacatgggg aactggtgat ccaggatata agatccctcc agaatttagt gctcttaaac
     1141 ataaaaaata catagtttct atggctcgtg gatctgatat taacagtgca ggctctcaat
     1201 tcttcattat gcttggagat gccccgtggc ttgataatgc atatacaata tttggcgagg
     1261 taatatctgg tcaagatgct gttgataaaa ttgcgttatt agaaacaaat tcagaaaatc
     1321 aaccaattga tattgaatct gcacgcataa aacaagtaat tatcagatcg caagaacctg
     1381 aaacctaatt ttccacaaat aaattatcct aaattacttg tatctgtcgg aaatacgata
     1441 tcttgcatat ttgtcatctg gtgaatactt gcctggatga actgtaactg ttttattgcc
     1501 gcacattgga cactcatcct ttaaggtgta tcttttacaa tctgggcatt tgcgcatctg
     1561 aaatctcatg ttatccttct cttgtcttct ttgactcttc tctagtgaat ttgtaagtgc
     1621 cacctttttt ttctatggtt gtttgaattg ttaaaagtac aggtttgaga gctttttcag
     1681 ctgttttgaa gttttgggca gaaactgtga gtcgatattt tggagcaccg atataagtta
     1741 tttctacact tgcatccttg ctttccatgg cttcaagaag ggattttttt atgatctcta
     1801 ttccattaga ttttgtacta gtcaattcta ggacacctcg tatctctaca tgtggaactt
     1861 gaatctttga actgatttct tctattgttg aaagtgtttt tttgggcaga ttaagatcat
     1921 ctaatacagt aattcctttt gatacgattt cagaaaatgc atcgtaaata gagttaaact
     1981 tgttaaccaa aacaccttct aatttttctg cttctgcttg agtaagacca gcctttgtcc
     2041 tcaaactatc gataaggatt tgctctttct catttctttt tagttctaaa agtttctttt
     2101 tcttttgatc cgaggaaact tgttttaaag ataaatctat ttcagatctt gcagtattaa
     2161 ctctttttac cagcaatact tttttttctc ctaccttgac aaatttttct acatgcctaa
     2221 tccaacctgt tgcaatttct gatatgtgta aaaacccctg aatgttgttg tattcatcaa
     2281 gactaacata tgcaccctga tccataattc ttgtaactgt agcaacaaca atctctccaa
     2341 cttcaggcag ctcctgaatt tcagtggtca taaatcctca aacttgggat acgtaattta
     2401 atgttctcat taaaaatcaa tccccttctt ggccttgatt cctgatttga aagggtgttt
     2461 tatttccttc atctcagtca cgagatctgc tgcatctatg atttcttgct ttgcatgatt
     2521 tcctgtgagg actaaattca aattggcagg tttgattttt atcaaatcta aaacatcttc
     2581 aatttttact aatcccaaat tgacagcata attaatttca tcaagtatca tgatatcata
     2641 tttttctgat tctatttttt ctctgcttaa acgaagagcc tctattgcaa ttttttcatg
     2701 ttctgcatgt ggacttttat catcaattat tccaacaaaa cctttaccaa tcgcagtaag
     2761 ctcaaactct ggttctaatc tttttgacga agtcatttcg ccatagtgcc aagttccctt
     2821 gatgaactgt atcatacaaa ttttgtaatt gtatcccact gctcttaatg ccatgcctag
     2881 tgcagcggtg gtttttcctt tgccctttcc agtataaaca atgactaatc catcttttcc
     2941 cattcttttt ttgggttcaa ctgtaaacta aaaagatttg gggtgctgga agacattcta
     3001 ctgtcaaaat ttaccttaaa attatgaatt aggtaaaatt tttcccataa gattctgcgt
     3061 atatagaata tggttgtaga ccgtctatgg cagaagcagg tctgggttat tttggtgcca
     3121 tgataggtgt catgatcgct atggctgtag tttgctttag ccttcgtggc aagagacctc
     3181 atgggccttc tgtagaacaa tctgggacat aatcggcatt tgaataagta gtgccgtttt
     3241 tctagtgtaa tctatgaaaa taaattttga agagaataaa atgagtagtt tataattatc
     3301 gttttaaaaa cgtgaaattg atgacaagag aatacacata tgatgtcgta atagtcggag
     3361 ctggacctgc tggttcgtca gccgcatggg ctgctgcaaa aaaaggagta cgtgttgcac
     3421 ttcttgaacg agaagaagca atagcacaaa gcatcagaac aagtggcgta acatggatta
     3481 atgatatgaa aaaattcaac attacatcgg attactataa tcctatcaaa aactactcat
     3541 ttcattctct aaataatcat gtaactgtac acggttctga atatcaagct gcagttttag
     3601 atgttagacg aacataccag tttcttgcat gtgaggcagc aaatgctggc gcagatattt
     3661 ttgtacgaac caatgcaact aatccattaa ttgatagcaa aggaaatctg gttggaataa
     3721 aagccacatc tttaaaagat gatctaatat ttcattgtaa gcttgtaatt gatgcaagtg
     3781 gattctattc ggttataggt aaaagcattg gcattgttcc acaatggaaa agatttggag
     3841 ttggagcaga gtatgaggca tatgttgaaa aagttgatcc agaaacatgg tccttgatgg
     3901 ttggacaaca atattctcca gcaggatatg catggatttt tccattggga aaaaataaag
     3961 taagaatagg agttggtata ggaaagcctg aatctaaaat cgatcctact cagcgtctcc
     4021 ttgaacttgt agaaaaaaaa ccaggcccac ttggtcaatt aggtaaaata actcctatag
     4081 aatttcatta tggtttgatt ccaaatgaag gattgacaca taccaccatc tataataaca
     4141 taatattggt tggcgatgca gcagggcaag caaacccact agttctagaa ggaatcagat
     4201 atgcaattga atttggcaga atagctggac aggtgggggc agaatctgta atatctggtg
     4261 atacatcaaa aacatcatta aaaccatatg aagatgcatg gaaaaaggca atcagctcaa
     4321 aaatcaaagc tgcaattaaa gtacagaata gatggttgaa tcttactgac gaagaatggg
     4381 ataaagaact tgaaataatt aaagaattat ctccagatga atttcttgat ttcataaaag
     4441 cagattttag catctcaagt atgattggcc ttgccacaca tcatccaaaa ctagccgtta
     4501 ggcaactttt tgacattgta aaaagcattt caggaacaca tcaatgaaga caaatcgctc
     4561 aaacatcaat ttaaataaaa ttctgtaata ttgatgagaa ttgaatatct caagaatcgt
     4621 acttgcaggc acaactagtg gtgttggcaa aacatcaatt actgcagcaa taatacatgg
     4681 aataaaaaaa cgcggatatt ctgtacaatc atttaaggtt gggcctgatt ttatagatcc
     4741 cagctatctt tctg
//
LOCUS       DATIDL010000094         2609 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrosopumilaceae archaeon isolate SMAG_U16342
            k141_1529353, whole genome shotgun sequence.
ACCESSION   DATIDL010000094 DATIDL010000000
VERSION     DATIDL010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935836
            Sequence Read Archive: SRR25158326
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosopumilaceae archaeon (soil metagenome)
  ORGANISM  Nitrosopumilaceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrosopumilales;
            Nitrosopumilaceae.
REFERENCE   1  (bases 1 to 2609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/04/2024 05:09:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,520
            CDSs (total)                 :: 1,474
            Genes (coding)               :: 1,474
            CDSs (with protein)          :: 1,474
            Genes (RNA)                  :: 46
            rRNAs                        :: 1 (5S)
            complete rRNAs               :: 1 (5S)
            tRNAs                        :: 43
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2609
                     /organism="Nitrosopumilaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1529353"
                     /isolate="SMAG_U16342"
                     /isolation_source="soil"
                     /db_xref="taxon:2202732"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(15..120)
                     /locus_tag="VLB45_04085"
     tRNA            complement(join(15..50,68..87,102..120))
                     /locus_tag="VLB45_04085"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(69..71),aa:Met,seq:cat)
     gene            203..1294
                     /locus_tag="VLB45_04090"
     CDS             203..1294
                     /locus_tag="VLB45_04090"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016940015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HSD04915.1"
                     /translation="MDKKIRIIGIIAAVAASVAIILSPSDPVVIPQPVTTPTGTKSVE
                     IVATNLQKPWAMDFTDDRIFLTEKVGRIRVIDSGSLVEEPLANLRVADVADGGLMGIA
                     LHPDFENNHYLYAFYTYSEDNKLWNKIIKITESENKLVDATVILDKIPGAEFDNGGVI
                     KFGPDKKLYIGIGDATDEHAAQDLQSPLGKILRLNDDGTIPADNPFSGSAVFSYGHRN
                     PQGMAWDESGNLYMTEHGPTKNDELNLVKAGQNFGWPEQECGGHEGFVDAISCYNPSL
                     EPAGMVFYSSDKLDLGNDLIMASLRGTNLYQITVKGDETSQKSILGGVGRIRDVNEGP
                     DGYLYILTGNTDGRGFPDKTDDRLLRIVK"
     gene            1296..2549
                     /locus_tag="VLB45_04095"
     CDS             1296..2549
                     /locus_tag="VLB45_04095"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004420 - hydroxymethylglutaryl-CoA
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0016054 - organic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HSD04916.1"
                     /translation="MADSSIPKFYEKNRKERIKSIASFCGLSKQDVKILEGHGGISFN
                     EADKMVENAVGTVSFPLGIATSFRVNKKDYLIPMVIEEPSVIAAASKAAKIARKHGGF
                     TMKADESYSIGQIQVVGVDVKSAIPKIMKFSKEILKLANSKSKTLSKMSKGAKKISCK
                     KIKTDYGEMLIVELLIDVGDAMGANITNTMCEGVAPLIEKLSGGRVILRILSNYSTKR
                     LVRGTATFDKNEIGGKKIVDNIILAYQFAAHDPYRAVTHNKGIMNGIIAVANATGQDT
                     RAIEAAAHAYASRTGRYTSLTEWKKDKKGNLVGKIDLPMSVGIVGGIVNVHPMIKICA
                     KILGVKSAKELACIIAAAGLAQNFSAIRALASEGIQKGHMKLHARNIAASAGVKPNLI
                     DEVVKRMVKEGNVSMHRAKEILKEF"
BASE COUNT          897 a          453 c          573 g          686 t
ORIGIN      
        1 atattggaat gaaatagcgg ggagtagatt tgaactactg atttgcgggt atctcatcag
       61 tgttgattat gagcccgccg ggatgacttt gccccaatag tctggcttcc ccaccccgct
      121 aattgaaaag aagtttcggg gtgatatata cgctttatct aattttggaa ataattaata
      181 ggatttacaa actgtctcaa acgtggacaa aaaaattaga ataattggaa ttatcgcagc
      241 agttgcagct tctgttgcaa taattctatc accatcagat ccagtagtga ttccacaacc
      301 agttaccact ccaactggaa caaaatctgt agaaattgtt gcaaccaatt tgcaaaaacc
      361 ttgggcgatg gattttacgg atgaccgaat atttcttaca gaaaaagttg gcagaatacg
      421 tgtcattgat tctggtagtc ttgttgaaga gccacttgca aacctacgtg tagcagacgt
      481 ggctgatggt ggcttgatgg gcattgcact acatcctgat tttgagaata atcactacct
      541 atatgcattt tatacatatt cagaggacaa caaactctgg aataaaatta tcaaaataac
      601 cgaatcggaa aacaaacttg tagatgcgac agtaattttg gataaaatac ctggtgcaga
      661 atttgataat ggtggtgtga taaaatttgg tcctgacaaa aaactataca taggaattgg
      721 tgacgccaca gacgaacacg ctgcacaaga tttgcaatct cctttgggaa aaattttaag
      781 gttaaatgat gatggcacca tacctgcaga taatccattt tcaggctcag ctgtgttttc
      841 gtacggacac agaaaccctc aaggcatggc ttgggatgaa tcaggtaatc tctacatgac
      901 agaacacggt ccaacaaaaa atgacgagct taatcttgta aaggcaggac aaaactttgg
      961 ttggcctgaa caagaatgtg gaggacatga gggatttgtt gatgcaattt cttgctataa
     1021 tcctagccta gagcctgcag gtatggtttt ttattcttct gacaaacttg atcttggaaa
     1081 tgatctgatc atggcatcat tgcgtggaac aaatctatac cagatcacag taaaaggcga
     1141 tgaaacttca caaaaaagca tattgggtgg ggttgggcga attagagatg tcaatgaagg
     1201 accagatggt tatctttaca ttcttacagg aaatactgat ggaagaggat tcccagacaa
     1261 gactgatgat agacttctta ggatagtaaa ataaattggc cgactcgtca atcccaaaat
     1321 tctatgagaa aaatagaaaa gaaaggataa aatcaattgc gtcgttttgt ggtctctcaa
     1381 aacaggatgt taagattctg gaaggacatg gtggtatatc atttaatgag gcagacaaga
     1441 tggttgaaaa tgcggtagga acagtttcat ttccacttgg aatagcaacc agtttccgtg
     1501 taaacaaaaa agactatctc attccaatgg taatcgagga gccatcagtc attgcagctg
     1561 cctccaaggc agccaagata gcaagaaaac atggaggttt taccatgaag gctgatgaat
     1621 catacagcat cggtcagatc caagtagtag gcgttgatgt aaaatctgcc attccaaaaa
     1681 taatgaaatt ctcaaaagag attctaaagc ttgccaactc aaaaagtaaa acattatcaa
     1741 agatgagtaa gggagcaaaa aaaatctctt gcaaaaagat caaaactgat tatggagaaa
     1801 tgttaattgt agaacttttg atagatgtgg gagatgcaat gggtgcaaac atcacaaata
     1861 caatgtgtga aggtgttgca cctctaattg aaaagctatc aggtggcaga gtgattctca
     1921 gaatactttc aaactattct actaaaagat tggttcgagg tactgctact ttcgacaaaa
     1981 atgaaattgg tggcaagaaa attgttgata atatcatttt agcataccaa tttgctgcgc
     2041 atgatcctta cagagctgta acccacaaca agggaatcat gaatgggata attgcggttg
     2101 caaacgccac tggacaagac acaagagcga ttgaggcagc tgcgcatgcc tacgcttcta
     2161 gaactggaag atatacctct ctgacagaat ggaaaaaaga caagaaaggg aatttggttg
     2221 gaaaaataga tcttcccatg tctgtgggaa ttgtgggggg aattgtaaac gtgcatccga
     2281 tgatcaagat ctgtgcaaaa attcttggag taaagtctgc aaaagaactt gcatgcataa
     2341 ttgcagcagc aggacttgca cagaacttta gtgcgatacg agctcttgct tctgaaggaa
     2401 tacaaaaagg acacatgaaa cttcatgcaa gaaacattgc tgcaagtgca ggtgtcaaac
     2461 caaacctgat tgatgaggtg gtaaaaagaa tggtaaaaga aggaaacgtc tcaatgcatc
     2521 gagctaaaga aatactcaaa gaattctagt gctcaagata tatatctaaa aaccctcgct
     2581 aggactacgt tggctttagt taaatttgc
//
LOCUS       DATIDL010000095         9190 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrosopumilaceae archaeon isolate SMAG_U16342
            k141_923077, whole genome shotgun sequence.
ACCESSION   DATIDL010000095 DATIDL010000000
VERSION     DATIDL010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935836
            Sequence Read Archive: SRR25158326
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosopumilaceae archaeon (soil metagenome)
  ORGANISM  Nitrosopumilaceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrosopumilales;
            Nitrosopumilaceae.
REFERENCE   1  (bases 1 to 9190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/04/2024 05:09:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,520
            CDSs (total)                 :: 1,474
            Genes (coding)               :: 1,474
            CDSs (with protein)          :: 1,474
            Genes (RNA)                  :: 46
            rRNAs                        :: 1 (5S)
            complete rRNAs               :: 1 (5S)
            tRNAs                        :: 43
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9190
                     /organism="Nitrosopumilaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_923077"
                     /isolate="SMAG_U16342"
                     /isolation_source="soil"
                     /db_xref="taxon:2202732"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..705)
                     /locus_tag="VLB45_04100"
     CDS             complement(<1..705)
                     /locus_tag="VLB45_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HSD04917.1"
                     /translation="MGEKMADLVLKNCTLVNVYSREINPKTQVAIFKDRIAYVGTDAS
                     HTVGENTVVIDLANRYVTPGFADPHTHIDKYVLPSELAKKSLLCGTTSIFSDPIDIVS
                     VCGYKGFRELVKHTENLPIRFFHVIPGGLPVDQKFSHAKTLTSTEERASLRLENVLGL
                     GEVFSWTKVTSQDAKTMHTLKYMLEKGCVINGHTAGASDKKLNAYIASGILSCHEPID
                     FDQVIERLGLGMSIMMR"
     gene            731..1111
                     /locus_tag="VLB45_04105"
     CDS             731..1111
                     /locus_tag="VLB45_04105"
                     /inference="COORDINATES: protein motif:HMM:NF004425.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L44e"
                     /protein_id="HSD04918.1"
                     /translation="MVESELDLTSKESVLDTIIDSKRCLNYGQARSSFMNMPKEVRRY
                     CPKCKKHTLQKVSIYKAGKRRGSAIGERRHAEDKKGYGGQKFPKLAKPAKTTKKATQI
                     LTCPECKKKFNKPGIRLRKFELVA"
     gene            1111..1308
                     /locus_tag="VLB45_04110"
     CDS             1111..1308
                     /locus_tag="VLB45_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S27e"
                     /protein_id="HSD04919.1"
                     /translation="MKKGQILIPKPNSKFQRVQCNECNEESVVYSHVATNVTCKSCGN
                     LLAESTGSIAKINGKILGSAE"
     gene            complement(1291..1875)
                     /locus_tag="VLB45_04115"
     CDS             complement(1291..1875)
                     /locus_tag="VLB45_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HSD04920.1"
                     /translation="MIGLVMCGGKGTRMEIPQEKLLLKYKDPIIQHVITALHDSGCFS
                     KIVAATSHNAPRTWEFLTNLGIFTMDTAGKGYVYDLNQILHNFDELVFVTSGDLPLLD
                     AEIIKKIVQLVDNDHTWTTVLISKDFLDSLHLKPEYTVIYEKKQYSYSGVSIVNPNKL
                     TKMESIEESYVILDDKRIAFNMNTKEDYTLLGTA"
     gene            complement(1872..2597)
                     /gene="cobS"
                     /locus_tag="VLB45_04120"
     CDS             complement(1872..2597)
                     /gene="cobS"
                     /locus_tag="VLB45_04120"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HSD04921.1"
                     /translation="MLKGISSVISFLTIIPSKNVELDTVAKNMYLFPIGGAIIGLIIG
                     AAGFGLSSFLEPMIVGLLLTGSVVILTGIHHTDALCDFADGIMAKGTKEKKLQVMRDP
                     AVGSAGVITIVLYAGGMIMAFSMMKGFTLFQAILLSELLAKFVMVLQANRGSSAWQGL
                     SSPFTQSMKDKKKLGIAAMMTVIPIVIIGGMMGVVVLLVGVGLSFVLLAAANRSFGGV
                     SGDVFGATNELVRLSSLLIFASL"
     gene            complement(2590..3582)
                     /locus_tag="VLB45_04125"
     CDS             complement(2590..3582)
                     /locus_tag="VLB45_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HSD04922.1"
                     /translation="MILITISSVILAIAIDLALGDPKNKFHPTVWVGTLIGKLVPLTR
                     NKSTKTEKLAGTAIVISIASLIAVLLSSLNIGLDLIEPANYDSELDIVITIISVIIMS
                     IMLKTTIAIKGMERHAKLIMSALSNNDLDDARTKLSMIVKRDTKSLDRQHIISGTLES
                     ISENTVDGITGPLFYFALFGLPGAFVYRTINTIDSMVGYKNEMFRNIGWFGANCDKTL
                     NYIPSRLTSLVMILAAMILGADWRHSLKIMREDRSKTESPNAGYPMATLAGALGVRFE
                     KIDHYVLGDGKLEFTEAHFKSAVSIMKLTAVLFCTIFTIPMIIMLSYFGWWLHA"
     gene            complement(3579..4421)
                     /locus_tag="VLB45_04130"
     CDS             complement(3579..4421)
                     /locus_tag="VLB45_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HSD04923.1"
                     /translation="MTKALMIQGTSSGAGKTTLVTALCRIFANSGYSVAPFKSQNMSS
                     YSYKTGDFEISRAQAIQAIAARTDIDPHMNPILLMPLGNYQSKVMIKGKFYKKMYAKE
                     YYKKFALSKGLQIAKESLDWLKQNNDLIIIEGAGSPAEFNLEKYDIANMRMAEYSDAP
                     VIIVADIDRGGSFASIVGTLELIDKKYRKFIQGFVINKFRGDMEILRPSFHKLKQKTR
                     KPVLGTIPLIKFNIPEEDSLHSNPKQVQWNKKYLKTLDVEIENLSRIVKSSLNMKAIG
                     GLLK"
     gene            complement(4423..5505)
                     /gene="hisC"
                     /locus_tag="VLB45_04135"
     CDS             complement(4423..5505)
                     /gene="hisC"
                     /locus_tag="VLB45_04135"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HSD04924.1"
                     /translation="MKFNLEKNISNHIPVPHGGIYSVIDQIDSKVIDFSSNVNPLGFP
                     DSVKDIIKKNISLLSVYPDPNSSKLKADLEKYTGVSKNQIIVGNGATEIIYNFCKAFI
                     SKNSQVVVPIPTFGEYEAATKLQGGRVYYFKTMNLNKDLFALQNNVTKKNTIFLCNPN
                     NPTGVLTSKKNMIKILENAYDKSRLVFLDECFIELVPESNESMVSYLKEFDNLFILRS
                     LTKSFGLAGLRVGYGLGSKKIIDTLTRIKIPWNVNGLAQKAASAAISSRSHLDKTRRL
                     IKKELTFLTKSISKIPKFYCYPSSTNFILIKSQINSKTLQKKLLEKKILVRDCSTFYG
                     LDNRFIRIAVRTHKENVKLVRSLETI"
     gene            5589..6665
                     /gene="asd"
                     /locus_tag="VLB45_04140"
     CDS             5589..6665
                     /gene="asd"
                     /locus_tag="VLB45_04140"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HSD04925.1"
                     /translation="MKRVAIIGVTGAVGQEFVVALNNHPWFEVKQIAASERSAGKKFV
                     DAIRDTNSGILKWHIRENIPEYVKNMIVESVDDIKPENLDLIFSSIESDAAREIETRF
                     AKDVPIISTSSAYRYESDVPILIPGINDDHAELLNVQRKNRGWKGFVAPLPNCTTTGL
                     AITMKPLLDTFGAQKIIMTSMQAMSGAGRSPGVSALDITDNIIPYIPKEEDKVRIETG
                     KILGKLSNGKIESANLKVSCTCTRVPVIDGHTESVFVETAKHADPESVKKAMEKFSEN
                     VSIAGLPSAPKNYLTVHDDPTRPQPRLDREVDGGMSTVVGRLREDSVFENGIKYMLFS
                     HNEKMGSAKGAVLLAEMLYKKGMI"
     gene            6774..7223
                     /locus_tag="VLB45_04145"
     CDS             6774..7223
                     /locus_tag="VLB45_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HSD04926.1"
                     /translation="MDELDLTILSELAADANISVPRISKKINVNSSVVYSRIKRLIKR
                     KLIERFTIVVNDRELGYTVKALTGINMDSKLRDNVIEELFKIPEVREISEVTGRFDIL
                     VTMYARNLDEMHRLISEKLGRIQGVQRSESFIEMKRTTKPMPYKPMK"
     gene            7279..8208
                     /gene="cyoE"
                     /locus_tag="VLB45_04150"
     CDS             7279..8208
                     /gene="cyoE"
                     /locus_tag="VLB45_04150"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010192136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HSD04927.1"
                     /translation="MLTSKTSSRIKVYYELTKPKIWYLLVFTAFGAAITASNIYDIEI
                     SPLTWALMIGGVAAGSAAANTLTNYHDRDIDAIMERTKNRPIPSRRIFPAEKARDFGL
                     ALAGISLAAAFGISFTTTLEQGLWALGFMAFGLMNNVLVYSYALKRHSRSNIILGGLC
                     GGSPPMIGYVAVTMQGLWDLGLVMAGLVFIWIPMHIWALTLHFKEDYNKVNVPMLTAV
                     QSEKTSARVIAGSTVMMVLFSIVPFFLTSDIGQPVMHEVYLYTAIATGVLMVALSAWV
                     VVKPTEKASWMLFKFSSPYLAVLFIALMIDSAL"
     gene            complement(8250..>9190)
                     /locus_tag="VLB45_04155"
     CDS             complement(8250..>9190)
                     /locus_tag="VLB45_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008300139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HSD04928.1"
                     /translation="TLNQEKNTQNSSFHRLLPLDRRHFSVRMVEGRHLHRSFMYAKEH
                     GEDLKVAITVGVHPAVSIAGAYQAEWGKDELEIANTLLENKMTLAKTPYSRMKVPSNA
                     EIVMEGKILHDKTHKEWMVEMLRTYDFKREQPVFELENLYFRNNAIFHDILSGYSEHR
                     LLMGMPIEAKLNRDVKKLLPQTKKIVMSDGGCNWLHAVVQIAKKKDSDAKKVIEKTFA
                     THRSLKMVTVVDEDIDPSDAVSVEYAMATRFQADKDLTIIKKIRGSSLDPSSDQKKLL
                     TAKLGIDATRPLSKRPEGFEIAKIPRENKISLKDYL"
BASE COUNT         2817 a         1670 c         1701 g         3002 t
ORIGIN      
        1 cctcatcatt attgacatgc caagcccaag tcgttcaatg acttggtcaa agtcaatggg
       61 ttcgtgacag gaaagaattc cagaagctat gtaggcgttt agttttttgt cactcgcacc
      121 tgcagtgtga ccatttatga cgcagccttt ttccagcatg tatttgagag tgtgcattgt
      181 ttttgcatct tggcttgtca cttttgtcca tgaaaatact tcccctaatc ccaatacatt
      241 ttccaaacgc agtgatgccc tttcttctgt agaagtaagt gttttcgcat gactaaactt
      301 ttggtcgact gggagccctc caggaatgac atgaaaaaat cttattggca agttttctgt
      361 atgcttgaca agttctctga aacctttgta tccacaaaca cttacgatgt caattggatc
      421 agaaaaaata gatgtagtgc cacagagaag agattttttt gcaagctcag atggcaaaac
      481 atacttgtca atatgtgtat gtggatctgc aaaacctgga gtaacatatc tgttcgctag
      541 atcaatgaca acagtatttt ctcctactgt atgcgatgca tcagttccaa cgtatgctat
      601 tctgtctttg aaaatggcaa cttgtgtttt gggatttatt tctctagaat acacattaac
      661 cagtgtacaa tttttcaaaa ctagatcagc catcttttct cccatggcta cagagtttag
      721 cgatgaaatc atggtagaaa gcgaacttga tctcacgagt aaagaatcgg ttcttgatac
      781 tattatagat tcaaagcgat gtttaaatta cggacaagca cggtcatcct ttatgaacat
      841 gccaaaggaa gtcagaaggt attgtcccaa gtgtaaaaaa cacacgctac aaaaagtctc
      901 tatatacaag gccggaaaaa gaagaggctc ggctataggt gaaagaagac atgcagagga
      961 taagaaaggt tatggtggcc agaaattccc aaaacttgca aaacccgcaa agactacaaa
     1021 aaaggcaaca caaattctta catgtccaga atgcaaaaag aaatttaaca agccgggtat
     1081 aagactaaga aagttcgagt tggtagcata atgaaaaaag gacaaattct aattccaaaa
     1141 ccaaacagca agtttcaaag agtacagtgt aatgaatgta atgaagagag tgtcgtctac
     1201 tcacatgtag ctacaaatgt aacctgcaag tcatgtggaa atcttcttgc agaatcaaca
     1261 ggttctattg caaaaataaa tggcaagata ctaggcagtg ccgagtaaag tataatcttc
     1321 ttttgtattc atattaaacg caattctctt gtcatctagt atcacatatg actcctctat
     1381 agattccatt ttcgtaagct tgtttgggtt tactatagac acaccagaat aggaatattg
     1441 ttttttttca tagattacag tatattccgg tttcaaatgt aatgaatcaa gaaaatcttt
     1501 gctgataaga actgtagtcc atgtgtgatc attatcaact agttgtacga ttttttttat
     1561 tatttctgca tccaacagtg gcagatcccc cgatgtcaca aatactaatt cgtcaaagtt
     1621 gtgcaaaatc tggtttagat catagacata tcccttccct gctgtatcca ttgtaaaaat
     1681 tcctaaattt gttaaaaatt cccatgtcct tggagcatta tgactggtag ctgccacaat
     1741 ctttgaaaaa caacctgaat catgaagtgc agttattaca tgttgaatga tgggatcctt
     1801 gtacttgagg aggagtttct cttgtggaat ttccatgcgt gtgccttttc ctccacacat
     1861 gactagacct atcatagtga tgcaaaaata agaagagatg aaagtctcac aagttcattt
     1921 gtagcaccaa agacatcacc tgaaacacca ccaaaactac gatttgctgc agcaagaagc
     1981 acaaacgata agcctacacc caccagcaaa acaaccacgc ccatcatccc tccaattatt
     2041 actataggga taacggtcat cattgcagct ataccaagtt tctttttgtc tttcatagat
     2101 tgtgtaaatg gtgagcttag cccttgccat gcagatgatc ctctgtttgc ctgtaacacc
     2161 ataacaaatt ttgccaaaag ttcacttagt aaaattgcct gaaagagggt aaaacctttc
     2221 atcatagaaa atgccattat cattccgcca gcatacaaca ctatcgtaat tactccagct
     2281 gatccaacgg ctggatcacg cataacttgt agtttttttt ctttggtacc ctttgccata
     2341 ataccatctg cgaaatcaca tagtgcatcg gtgtgatgta taccggtgag aattacgaca
     2401 gaacctgtca ataacaatcc tactatcata ggttctaaaa atgatgaaag cccaaatcct
     2461 gctgcgccaa taattaaacc aattattgca ccgccaatag gaaacagata catgtttttt
     2521 gcaacagtat caagttctac attttttgaa ggaattattg ttagaaacga aattacggag
     2581 ctgattccct taagcatgca accaccatcc aaagtatgaa agcatgataa tcattgggat
     2641 tgtaaagatt gtacaaaata gtactgctgt gagtttcatt atagatacag cagatttgaa
     2701 atgagcttct gtgaattcta actttccatc tcccaataca tagtgatcta ttttttcaaa
     2761 tcgtactcca agggctcctg ctagtgtagc cataggatat cctgcatttg ggctctctgt
     2821 ctttgatcta tcctctcgca ttatttttaa tgagtgtctc cagtctgccc ctaggatcat
     2881 tgcagcaagt atcatcacaa ggcttgtcaa tctagatgga atatagttga gtgttttatc
     2941 gcaatttgct ccaaaccagc caatatttct aaacatctca ttcttgtacc ctaccatgga
     3001 atcaatggtg tttatagtac gatatacaaa tgcacctggc aacccaaaca atgcaaagta
     3061 gaagagtgga ccagtgatgc catctactgt attttcactt atgctctcca aagtaccaga
     3121 aataatgtgt tgtcgatcca aactcttggt gtctcttttt actatcattg aaagttttgt
     3181 tcttgcatca tcaagatcat tatttgatag tgcactcatg attagtttgg cgtgtctttc
     3241 cattcccttt atggcaattg tagttttaag cataatactc ataattataa cagaaataat
     3301 cgtaattaca atatccaatt cagaatcata attagctggt tcaattagat ctaacccgat
     3361 gtttaaagaa gatagtaata cagcgataag agatgcgatt gaaattacaa tagctgtccc
     3421 tgcaagtttt tcagtttttg tagatttgtt tcttgtaaga ggtacgagtt ttccaatcag
     3481 tgttccaacc cagacagtgg gatggaattt gttttttgga tcacctaatg ccaagtcaat
     3541 tgctatagca aggattacag atgatattgt gattaaaatc acttgagcaa tccacctatt
     3601 gctttcatgt tcagactaga ttttacaatc cgacttaaat tctctatttc tacatccaga
     3661 gtttttagat attttttgtt ccattggacc tgttttgggt ttgaatgaag agagtcctct
     3721 tctggaatgt tgaattttat gagaggtatt gttcctagaa caggttttct tgtcttttgt
     3781 ttcagtttat gaaagcttgg gcgcagtatt tccatgtcgc ctctgaactt gtttattaca
     3841 aaaccctgta tgaattttct gtatttcttg tctatgagtt ccagtgtgcc aacaatgctt
     3901 gcaaagcttc cacccctgtc tatatcagca acaataatta ctggagcatc agaatattcc
     3961 gccattctca tatttgcaat gtcatatttt tccaagttaa attctgcagg agaacctgcc
     4021 ccttctatta ttatcaaatc gttattttgt tttagccagt caagtgattc ttttgcgatt
     4081 tgtaatcctt tggagagtgc aaattttttg taatactcct ttgcatacat ttttttgtaa
     4141 aactttcctt tgatcataac tttggattga taatttccaa gcggcatgag gagaatcgga
     4201 ttcatgtgcg gatcgatatc tgtccttgcc gcaattgcct gtattgcttg tgctcgtgat
     4261 atttcaaaat caccagtctt gtatgaataa gaagacatgt tttgcgattt gaatggtgca
     4321 acactatatc cagagtttgc aaatattctg catagagcag taacaagggt tgtctttcca
     4381 gcaccggatg aagttccctg tatcatcagt gctttagtca attcaaatcg tctccaaaga
     4441 tctcacaagt ttcacatttt ctttgtgggt acgaacagca attctaatga atctattatc
     4501 aagaccataa aaagtactac aatccctcac tagaattttt ttttctaaaa gttttttctg
     4561 cagggttttt gaatttattt gtgatttaat cagaataaag ttagtcgaag agggataaca
     4621 atagaatttt ggtatttttg atatggattt tgttagaaaa gttagttctt ttttgattaa
     4681 tcttcttgtt ttatccagat gagatctgct tgaaattgct gcgcttgccg ccttttgtgc
     4741 cagtccattt acattccaag ggatctttat tctcgttagt gtatctatga tttttttgct
     4801 tccaagtcca tagccaactc tgagtcctgc tagcccaaat gatttagtca gagatctcaa
     4861 tatgaagaga ttatcgaatt ctttcagata tgataccatt gattcgtttg attctggaac
     4921 aagttcaata aagcactcat ccaaaaaaac tagtctggat ttatcatatg cgttctctag
     4981 aatttttatc atattttttt tcgatgttaa aacacctgtg ggattgtttg gattacaaag
     5041 aaaaattgta tttttctttg tcacattatt ttgcaatgca aagagatctt tatttaaatt
     5101 catggtttta aaataataaa ctcgtccgcc ttgtaatttc gtagcagctt catattctcc
     5161 aaatgtagga attggcacta caacttgact atttttgctt atgaaagcct tacagaaatt
     5221 gtagattatt tctgtagcgc catttcctac aatgatttga ttttttgaaa caccagtata
     5281 tttttcaaga tcagccttaa gctttgatga gtttggatct ggataaacgg aaagaagtga
     5341 aatatttttt ttaattatat ctttaactga gtctggaaat ccaagcgggt tcacgtttga
     5401 gctaaaatca ataactttgg aatcaatttg atcgattaca gaataaattc ctccatgcgg
     5461 taccggaatg tgattagaaa tgtttttttc tagattaaat ttcacaccat gaaatatcat
     5521 aatgggattt agtgtgtttt ggttattcgt gctaaaggaa tattaattgt ataaagcgga
     5581 tattacgcat gaaacgagtt gcaattatag gagttactgg agcagttgga caagaattcg
     5641 tagtggcact taacaatcac ccttggtttg aggtaaaaca aatagctgct tcggagcgtt
     5701 ctgcaggtaa gaaatttgta gatgctatca gggacaccaa tagtggtatt ttaaagtggc
     5761 atatcagaga aaacattcca gaatatgtta aaaatatgat agttgaatca gtagatgata
     5821 taaaaccaga aaatctggat ttgatatttt cttcaataga aagtgatgct gctcgtgaaa
     5881 ttgaaactag atttgcaaag gatgttccca taatcagcac atcttctgct tatagatatg
     5941 aatcagatgt accaattctc attccaggaa taaatgatga tcatgcagaa ctactaaatg
     6001 tacagcgaaa gaatcgtggt tggaagggct ttgtagctcc actgccaaat tgcactacca
     6061 cagggcttgc tataacgatg aaaccattac ttgacacatt tggagcccaa aaaattatca
     6121 tgacatccat gcaagccatg tctggagcag gtagatcgcc tggtgtttct gcattggata
     6181 taacagacaa cataattcca tacattccaa aagaggagga caaggttaga atcgaaactg
     6241 ggaaaatact tggcaaatta agtaatggaa aaattgaatc tgcaaatctg aaagtaagtt
     6301 gtacttgtac tagagttcca gtgattgatg gacatactga atctgtattt gttgaaactg
     6361 caaaacatgc tgatcctgaa tcagttaaaa aggcaatgga aaagttttcc gaaaatgtta
     6421 gcattgcagg actcccatca gcaccaaaaa actatctcac agtacatgat gatcctacaa
     6481 gaccacaacc aagacttgat agagaagtag atggaggtat gtctactgtg gtaggaagat
     6541 tgcgtgaaga cagtgttttt gaaaatggaa taaaatacat gttgttttct cacaatgaga
     6601 agatggggtc tgcaaaagga gcagtacttt tggcagaaat gctttacaaa aaaggcatga
     6661 tataatctga ctctgtttaa tccataattg atataaaatt tgcaaaaaaa atgtaataat
     6721 agctttatta accccacttt tattcacaat aattggtgtt ttgaacggca aaagtggatg
     6781 aattagatct tacaattctt tctgaacttg ctgccgacgc aaacatttct gttccacgca
     6841 tttcaaaaaa gattaacgta aactcgtctg ttgtatatag cagaatcaaa cgacttatca
     6901 aaagaaaact cattgagcgt tttactatag tagtaaatga tagagagctt ggctatacag
     6961 taaaagctct aacaggaata aacatggatt ccaagcttcg tgataatgta atagaggagc
     7021 tattcaaaat tccagaagtt agagaaattt ctgaagttac tggtagattt gatattttgg
     7081 ttacaatgta tgcaagaaat ctagatgaaa tgcacagatt gatatcagaa aagcttggtc
     7141 gtatccaagg agtacagcgt tctgagagtt ttatagagat gaaaaggacg accaaaccaa
     7201 tgccatacaa accaatgaag taatattgat tttttcaacc tataaatata aaacaacaac
     7261 caaaaaagga gagtcatcgt gttaacaagc aagacaagct ccagaattaa ggtatactat
     7321 gaattaacta aaccaaaaat ttggtatctt ttggttttta ccgcttttgg tgcagccata
     7381 actgcatcta atatctacga cattgaaatt tcaccgctaa cttgggcttt gatgataggc
     7441 ggtgttgctg caggatcagc agctgccaac actctgacga actatcatga cagggacata
     7501 gatgccatta tggaaagaac caaaaataga ccaattccta gcagaagaat ttttccggct
     7561 gaaaaggcca gagattttgg actggcatta gccggaataa gtctagctgc agcgtttggg
     7621 atatccttta ccacaacttt ggaacaagga ctttgggctt tgggttttat ggcttttgga
     7681 ttaatgaata atgttttggt gtatagctat gccctcaaaa ggcatagcag atccaatatc
     7741 attctgggtg gtctttgtgg tggatctcca ccaatgattg gatatgttgc agttaccatg
     7801 caaggactct gggatctagg actggtgatg gcaggacttg tgttcatctg gattcctatg
     7861 cacatatggg ctcttacttt gcactttaaa gaagattata acaaagtcaa cgtgcccatg
     7921 ttgacagctg ttcaatctga aaagacttca gctagagtga ttgcaggttc taccgtaatg
     7981 atggtgttgt ttagcattgt accatttttc ttgactagtg atataggtca accagtaatg
     8041 catgaagttt atctttacac tgcaatagct acaggagttt tgatggtagc tttgagtgca
     8101 tgggttgtag taaagcccac tgaaaaagca tcatggatgt tgtttaaatt ttctagtcca
     8161 tatctggcag tattgtttat tgcattgatg atagattctg ctttgtaact agtcaaaact
     8221 aggcactttt acagataaat aaagatcaat tatagataat cttttagcga gattttattt
     8281 tctcttggaa tttttgctat ctcaaaacct tctggtcttt ttgaaagagg tcttgttgcg
     8341 tcaatgccaa gctttgcagt aagtagtttt ttttgatcac ttgatggatc taggcttgag
     8401 cctcttattt tttttataat ggtcaggtct ttgtctgctt gaaaccttgt agccatagca
     8461 tattctacac taacagcgtc acttggatca atgtcctcat ccaccacagt taccattttc
     8521 aaagatctgt gcgtagcaaa tgttttttca atgacttttt ttgcatcaga gtctttcttt
     8581 tttgcaatct gcacaactgc gtgcaaccag ttgcaaccac catcactcat tactattttt
     8641 tttgtttgag ggagcaattt tttcacatct cggtttagtt ttgcctcaat aggcattccc
     8701 atcaacaatc tatgttcaga atatccagaa aggatgtcat ggaatattgc attgtttcta
     8761 aaatagagat tttctagctc aaatacaggt tgttctcttt tgaaatcata tgttctgagc
     8821 atttcaacca tccattcctt gtgagtttta tcatgtagaa tttttccttc cataacaatt
     8881 tctgcatttg atggcacttt cattctagag tatggtgttt ttgctaacgt cattttgttt
     8941 tccaatagag tatttgcaat ttctagttca tcttttcccc actctgcttg gtatgcacca
     9001 gctattgaca cagctggatg tactcctacc gtaattgcca ctttaagatc ttcaccgtgc
     9061 tctttggcat acataaaaga gcggtgcagg tgtcttcctt ctaccattcg tacagagaaa
     9121 tgtcgtctat ctagaggcaa aagcctgtga aatgaggagt tctgtgtgtt tttttcttgg
     9181 tttagtgtat
//
LOCUS       DATIDL010000096         2458 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrosopumilaceae archaeon isolate SMAG_U16342
            k141_319147, whole genome shotgun sequence.
ACCESSION   DATIDL010000096 DATIDL010000000
VERSION     DATIDL010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935836
            Sequence Read Archive: SRR25158326
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosopumilaceae archaeon (soil metagenome)
  ORGANISM  Nitrosopumilaceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrosopumilales;
            Nitrosopumilaceae.
REFERENCE   1  (bases 1 to 2458)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2458)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/04/2024 05:09:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,520
            CDSs (total)                 :: 1,474
            Genes (coding)               :: 1,474
            CDSs (with protein)          :: 1,474
            Genes (RNA)                  :: 46
            rRNAs                        :: 1 (5S)
            complete rRNAs               :: 1 (5S)
            tRNAs                        :: 43
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2458
                     /organism="Nitrosopumilaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_319147"
                     /isolate="SMAG_U16342"
                     /isolation_source="soil"
                     /db_xref="taxon:2202732"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..502
                     /locus_tag="VLB45_04160"
     CDS             <1..502
                     /locus_tag="VLB45_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="copper-binding protein"
                     /protein_id="HSD04929.1"
                     /translation="DGKTKFALTLVEPGNSMGTWQFAGNALAVHTKKTEPFTVSYSVS
                     YAEKPQSNTIKSGTLTSVQDPGLGHETHQLAIILAPISNVYKGVLTYSASENVQLVAL
                     HGPLAEGEDKGQPIWIPDGKTKFALTLVEPGNSMGTWQFAGNALAVHTKNTEPFTVSY
                     SVAAGQ"
     gene            complement(543..1079)
                     /locus_tag="VLB45_04165"
     CDS             complement(543..1079)
                     /locus_tag="VLB45_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007401814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HSD04930.1"
                     /translation="MEEANDVIILSAISKGAKKFDKIEKVTKITGKDLNDMLERLEKN
                     GMITIVEKKGLFGTKKEIVLTQKGTKELEERKFELEKEWDQMVTMWKGGDKQKLQQYM
                     ENKKSLLPSMMFLGIMDILMFSTMMSFMGMAMSSYIPQEQIPADAGAGAESTDSGDTG
                     SDFSGGDGGGGFDIDIGF"
     gene            1146..1565
                     /locus_tag="VLB45_04170"
     CDS             1146..1565
                     /locus_tag="VLB45_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HSD04931.1"
                     /translation="MRSVEQDSHTDEQIRKFYSLKNIAVVGMSKNPEKAAHYVPKYLA
                     DQGYNIIPVNPTADEIMGKKCFHEISQVQDSIDIVDVFRPSDQVLPVVEEAIKKKPKV
                     IWLQEGIHNQEAEELARKAGIEVVFNRCMLAEHERLF"
     gene            1569..1928
                     /locus_tag="VLB45_04175"
     CDS             1569..1928
                     /locus_tag="VLB45_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04932.1"
                     /translation="MTENEFSEFYEAVLKLAKSFEEKNMLIKVQAEPDANIIRIYGEK
                     TDSLVRAKAGLEEVAELAYTTAEHHPYWNLIYNASQISKLALEKWADTLTKEELDEIS
                     WCSDEIKNTAAKLKEKT"
     gene            1982..>2458
                     /locus_tag="VLB45_04180"
     CDS             1982..>2458
                     /locus_tag="VLB45_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HSD04933.1"
                     /translation="MPIKIGLLGKTNTGKTTFFNAATLSHSEISTYPFTTKKPEKALG
                     NAITPCVHPEFNVQDQPQNSKCVDGWRFIPIELIDLPGLIKDAWAGKGLGNQFLSVAT
                     QADAILHVVDVSGSVDASGKITDVGNGDPISDVADIEEELIMWYLKLLEGNRDKISK"
BASE COUNT          778 a          493 c          481 g          706 t
ORIGIN      
        1 tgatggaaag acaaagtttg cattgactct tgttgagcct ggaaactcta tgggaacatg
       61 gcagtttgct ggaaatgcgt tagcagtcca cacaaagaag acagaaccgt ttactgtcag
      121 ctactcagtg agttatgcag aaaaaccaca atctaacact ataaaatcag gaacgctgac
      181 atctgtacaa gatccaggtc tgggtcatga aacccatcag ctggcaataa ttctagctcc
      241 aatctcaaat gtctacaaag gagtcctgac ttactctgca tctgaaaacg ttcagcttgt
      301 ggcactacac ggtccacttg cagaaggtga ggataaagga cagccaatat ggattcctga
      361 tggaaagaca aagtttgcat tgactcttgt tgagcctgga aactctatgg gaacttggca
      421 gtttgctgga aatgcattag cagtccacac aaagaacact gagccattca cagtgagtta
      481 ttcagtagct gcaggacaat agtccaaagc cattctttat tttttatttt tattattttt
      541 aattagaatc caatgtcgat gtcaaatccg ccaccgccat ctcctccgct aaaatcagag
      601 ccggtgtcgc cgctgtctgt actctctgca ccagcacctg catcagctgg tatctgttct
      661 tgtgggatgt agcttgacat tgccatgccc atgaagctca tcattgttga aaacatgagt
      721 atgtccatta ttcccagaaa catcattgat ggaagcaatg attttttatt ttccatgtat
      781 tgctgaagct tttgcttgtc tccgcctttc cacatggtaa ccatctggtc ccattctttt
      841 tcaagctcga attttctttc ttctagttct tttgttcctt tttgagtcaa cacaatttct
      901 tttttggttc caaagagtcc ctttttttct actattgtaa tcattccatt tttttctagt
      961 ctctcaagca tgtcgttgag atctttgcct gtaatctttg tgactttttc aatcttgtcg
     1021 aacttttttg cacctttact aattgcgctt agaattatga catcgtttgc ttcttccata
     1081 atttttgaat agaaaaaatc gtcctataaa tcgttcccta taagatatta aagccaactt
     1141 gccatatgcg atccgtggaa caagattcgc acacagacga acaaataaga aaattttatt
     1201 cattaaaaaa catcgcagta gttggcatgt caaaaaatcc agaaaaagca gcacactatg
     1261 tgccaaaata tcttgcagat caaggataca acattattcc tgtaaatcct actgccgatg
     1321 aaataatggg caaaaaatgt tttcatgaga tcagtcaagt tcaagattct atcgatatag
     1381 tggatgtgtt taggccatct gaccaagtct tgcctgtagt tgaagaggca ataaagaaaa
     1441 aacccaaagt aatatggctg caggaaggaa ttcacaacca agaagcagag gagcttgcac
     1501 gcaaggctgg aattgaggtt gtctttaaca gatgcatgct tgcagagcac gagagattat
     1561 tttagaacat gactgaaaat gaattttcag aattttatga agctgtacta aagctggcaa
     1621 agtcttttga agaaaaaaac atgctgataa aagtccaggc agagcctgat gcaaatataa
     1681 tccgaatata tggtgaaaaa acagactcac tagtcagggc aaaagcagga ctggaagaag
     1741 ttgctgagct ggcatatact acggcagagc accacccgta ttggaatctg atatacaatg
     1801 catcacaaat ctcaaagctt gcacttgaaa aatgggccga tactctaaca aaagaagagc
     1861 ttgatgaaat atcatggtgt tctgatgaaa tcaaaaacac tgccgcaaaa ctaaaggaaa
     1921 aaacctgaga tgattacaat ttcttcggca gagataaata ttcaatttct gaattcaact
     1981 tatgccaatt aagattggtc ttctcggcaa gacaaatact ggcaagacta ccttctttaa
     2041 cgctgcaact ctttctcatt ctgagatctc aacttatcca tttacaacaa aaaaaccaga
     2101 aaaagcacta ggcaatgcaa tcacgccgtg tgtccatccg gaatttaatg tccaagacca
     2161 accgcaaaac tccaaatgcg ttgacggttg gcgtttcatt ccaattgagc taattgatct
     2221 tccaggtctg ataaaagacg catgggctgg aaagggatta ggaaaccagt tcctttcagt
     2281 tgcaactcag gctgatgcaa tattgcatgt agtagatgta tctggaagtg tagacgcatc
     2341 tggaaaaatc actgacgttg gcaatggtga tccgatttct gatgttgcag atattgaaga
     2401 agagttgata atgtggtatc tgaagctttt agaaggaaac agggacaaaa tttcaaaa
//
LOCUS       DATIDL010000097        17570 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrosopumilaceae archaeon isolate SMAG_U16342
            k141_320032, whole genome shotgun sequence.
ACCESSION   DATIDL010000097 DATIDL010000000
VERSION     DATIDL010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935836
            Sequence Read Archive: SRR25158326
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosopumilaceae archaeon (soil metagenome)
  ORGANISM  Nitrosopumilaceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrosopumilales;
            Nitrosopumilaceae.
REFERENCE   1  (bases 1 to 17570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/04/2024 05:09:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,520
            CDSs (total)                 :: 1,474
            Genes (coding)               :: 1,474
            CDSs (with protein)          :: 1,474
            Genes (RNA)                  :: 46
            rRNAs                        :: 1 (5S)
            complete rRNAs               :: 1 (5S)
            tRNAs                        :: 43
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17570
                     /organism="Nitrosopumilaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_320032"
                     /isolate="SMAG_U16342"
                     /isolation_source="soil"
                     /db_xref="taxon:2202732"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            14..1105
                     /locus_tag="VLB45_04185"
     CDS             14..1105
                     /locus_tag="VLB45_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04934.1"
                     /translation="MNKKILVGVIAAGVIAVVIMTAFLSTQNTMLQKNEKLGLYILTP
                     SSTPTLSDLKEVYTEAASAGIGRSNVYLFWPTIEPQQGQYDWKIPDALMSLNKQNDLK
                     VTLYFSIINSRVFGPFPTWMGEPQLDESLKEKTVKILDDILSRYDIVDHVIIGGEIDA
                     YFRENDKDITKYEEFFTGVYDDLKERYPNVKFGNSFSLHGVLNHQQEDLVTKLNQGDF
                     VAFTYFPVNTLNEIDKTPEDAGKNLEKILDLAGNKQIALMEVSWSTAESVGGSNEGQV
                     KFMKIVYDFYRKNEPRFEFLSWFRQYDRPIDSCMKGLNTSFDISFGNEFVLQRTAEYV
                     CAAGLIDVDKHPKPAWEEFKRQVQFNPNS"
     gene            1133..2311
                     /locus_tag="VLB45_04190"
     CDS             1133..2311
                     /locus_tag="VLB45_04190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04935.1"
                     /translation="MRYLSFVLIALLFANLIPAFSDSLPPTKQEQDLYNKLLAEFEKL
                     NIEKKDVSDVLKTFEKPLVVTFPCDSTDINDLVVSVVETKLARFDEAQEHEGMITGGT
                     YTWKCTITTTSDGKITMDCDNELKLNHDLMTQDTGKDSRIKQAENLLILYHELLHGQL
                     MIEAIQSSEKWRNDVCNKSTDGKIDYSYSDKDHKIINPLQTEFLSQLITNSGGIMIVQ
                     EIMPEETEDGIFTKKLGNLHDYPQYVESGIHVTLRGSNLVDTEFSSQNSDIILSGSLA
                     NKTQSGIAWLYIFGKPDDKQETQIISKTEIHIPDWVKKNAGWWAANKISDSEFLGAIE
                     YLIQEGIIQVPETQKQQESNKIPNWFKQNAQWWYEEKIDDETFAKGIQYLISVGIISV
                     "
     gene            complement(2320..2393)
                     /locus_tag="VLB45_04195"
     tRNA            complement(2320..2393)
                     /locus_tag="VLB45_04195"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2356..2358),aa:Gly,seq:tcc)
     gene            complement(2415..3083)
                     /locus_tag="VLB45_04200"
     CDS             complement(2415..3083)
                     /locus_tag="VLB45_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012216008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis protein"
                     /protein_id="HSD04936.1"
                     /translation="MLSLVLAESALELIPRELQKHNSVLAHAKRVGKRPSEVLLDISW
                     HFAAMKGIKNEIKRGRPDLVHFCLLECCTIPLYFENKLAVYIHTIDDKVIFIGENVRL
                     PKSYHRFVGLIEKLYLEGKIEEGGKKLLELKDMKFVDLIDKIRPKEIVGLSIKGETSS
                     YEKFSRELDKDTCIVIGGFSKGHFSDAILERINRLVSVDKNSLEAHVVISRVLYECEK
                     RIIM"
     gene            3120..3428
                     /locus_tag="VLB45_04205"
     CDS             3120..3428
                     /locus_tag="VLB45_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase P subunit"
                     /protein_id="HSD04937.1"
                     /translation="MKIATRQIILERMEILIQNAVSNARKNPELAQRQASLAKKLSTK
                     HRVRMPYQIRMNYCKKCKKFIAPGVNARIRLGRSSVKSIRITCSFCNHTYRKIISKAQ
                     "
     gene            3460..3912
                     /locus_tag="VLB45_04210"
     CDS             3460..3912
                     /locus_tag="VLB45_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012216007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19e"
                     /protein_id="HSD04938.1"
                     /translation="MAKAYDVPADVLINKLSEILKSEEITAPAWAPFVKTGSHADKPP
                     QKNDWWYTRCASLLRKIYLHGPVGVKDLRGMYGGAIAVGYGGAHHRDAGGAIIRTAIH
                     SLEKLGYIDKVEGKGRTVSHQGMKKIDRVATEILNELIVKNPNLKKYS"
     gene            3922..4200
                     /locus_tag="VLB45_04215"
     CDS             3922..4200
                     /locus_tag="VLB45_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HSD04939.1"
                     /translation="MSNPNQGNEKEPTESEVASMKEQALRTLLEPQARLRLNNVRMVK
                     PELAAMVENYLIGMASQGRLNTQISDDQLKQILQSIQQPKRDFKINRR"
     gene            4244..5320
                     /locus_tag="VLB45_04220"
     CDS             4244..5320
                     /locus_tag="VLB45_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HSD04940.1"
                     /translation="MKAVVYEAYAPNDDYKSILKVKDIPEPKPKPDEVVFKVKAAALN
                     YNDIWGMRGQPVAVPLPHVSGSDAAGEVIAVGEDVTNFKVGDRVVSHSNMSCRVCAAC
                     TDGREFDCIKRTIWGFQTGPTWGAFSEITHLPEVNVAKIPEGVSFDEAAAASMTLLTS
                     WHMLVGRAKIRPGQTVLIMGGGSGVGSFGIQIAKLYNCTVIATASPDKLDKLKQLGAD
                     YVVDHRKEDWNKEVFKITKEISAKTGVAPGIDVSFDHIGQTHWNPQLTLLKYGGTLVS
                     CGATTGYDAKTDLRHIFFKGTNILGSTQGTKAELEQGLYWMAKGKIKAVIDSVYSFEN
                     AAEAHTKMLTGKGLFGKILMKPNA"
     gene            5329..6189
                     /locus_tag="VLB45_04225"
     CDS             5329..6189
                     /locus_tag="VLB45_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HSD04941.1"
                     /translation="MVRLLRSLIFVPGNNSRFLEKAKTLPADIICFDLEDSVPINEKN
                     VARNLIKNALTKHSKYKAEIYVRTNSPVSGMIPDDLSKIIQKGIDGIVIPKVNNAIEL
                     KRIEKLVTSLEKKRKLKPIEIIPSIESAEGVVNTYDIASSSKRIPAVVFGVFDLLNDM
                     GIEYTKQPEGAKYARAKIPIDTRAAGKYAIDAIWQDLNDENGLVKDCKIGKNLGYAGK
                     SIIHPNQIHIAHSIFKPNQDEIDWAKKVCQVYANSIKQSKGATIVEGKMIDEVHYKRA
                     KSLLDSIKNS"
     gene            6262..6852
                     /locus_tag="VLB45_04230"
     CDS             6262..6852
                     /locus_tag="VLB45_04230"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HSD04942.1"
                     /translation="MFTGIIEGLGIIKKFEKKTKTRSAAKMKVDLGDLAKGLKVGHSV
                     AINGVCLTVTKISKNQAEFEMINETIKKTDLGALESGDKVNIERSLKVGDRMEGHFVL
                     GHIDDTGKIIKIEKMPKEIKLWIEISKDLARHIVKKGSIAIDGISLTLVDVIKNKISV
                     CIIPHTMKITNLNSKKIGDKVNIETDILGKYTTKKI"
     gene            6924..7604
                     /gene="ribB"
                     /locus_tag="VLB45_04235"
     CDS             6924..7604
                     /gene="ribB"
                     /locus_tag="VLB45_04235"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HSD04943.1"
                     /translation="MSLDVAISSLQRGEFVLLHDAGKRENEVDMVVAAEFVTPAHIAR
                     MRQYAGGLVCLAINDSFAQNLGLKYMHDILSNSDDVDIESKKMIMGKAPYGDQPSFSI
                     SINHAQTFTGITDRDRALTIKEMANLSKTFNENKKYEFISSFKTPGHVPVLIAKNGLL
                     SKRLGHTEMSVYLMELAKLSPTAAICEMLDSESYTALSVEKAKKYAKENAIPFLEGKD
                     LLEFSKVH"
     gene            7607..8020
                     /gene="ribH"
                     /locus_tag="VLB45_04240"
     CDS             7607..8020
                     /gene="ribH"
                     /locus_tag="VLB45_04240"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HSD04944.1"
                     /translation="MNIAIVVSEFNQEITSKMFEVALEKAKNLKLNIKYTCQVPGVFD
                     MPVVIDSLLQKKDIDAVVTLGAVIKGQTKHDELIANVTAKIIAELSIKYQKPVTLGIT
                     GPGMTDKQAQARIRPVAERAVEATEKIANQLRKIR"
     gene            8017..8766
                     /locus_tag="VLB45_04245"
     CDS             8017..8766
                     /locus_tag="VLB45_04245"
                     /EC_number="3.5.4.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase IIa"
                     /protein_id="HSD04945.1"
                     /translation="MIQLTILKITGYGPWTLTLGSDREHRLQILQSSLYKEIQNLFSE
                     KNCLVFSNRFDELFAITNGLILSDHVEIQKKLKESFELDLSMSIGCGESPFDANLQAY
                     EARKSKIFLNEELNIFGFTDGKTEQNVQIMHIDVDGSTSISTKKSPYEISLLMFKLYS
                     EMSEFFLKQNYLSFFMGGDNFMIVTSGNGQENASKFLDLIEKKYNITMNCGIGTAKTA
                     REAAKLATKSLDTIREMRDSGKTQRVLELTC"
     gene            8760..9953
                     /locus_tag="VLB45_04250"
     CDS             8760..9953
                     /locus_tag="VLB45_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HSD04946.1"
                     /translation="MLIKNASVLYGNELNYIQNTNIRISDNLFSKIGTNLKPLTKEET
                     HDCEGLLMIPGFINAHTHIGDSIAKDIGIDYGIEEKIHPVMGFKHKILKNTESSHLTS
                     FMRNSCISMMKKGITTFIDFREGGLDGILILKKAISNLPIRSIILGRIDYYQNTSSIK
                     KNIPLSASKKKELTKLLEKCDGVGISGPNENSNSVLRHYSQTKKIKAIHAAETIGSCE
                     ISKKSTGKTETERAMILKPNFLVHMTHASKHDLVLAAQNKISIVVCPRANASLADGIP
                     NVHLMMKAGCNLAIGTDNVMINSPDMFREMDYLWKISMGMQKTRLSPKSILKMASVNV
                     SKMLKNKQLGTISPQSFADCIFIDKHAIDLEPMHNPHASIVHRASESTIKAVMIGGRI
                     VHGKI"
     gene            9940..10593
                     /locus_tag="VLB45_04255"
     CDS             9940..10593
                     /locus_tag="VLB45_04255"
                     /EC_number="1.1.1.302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018039325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-diamino-6-(ribosylamino)-4(3H)-pyrimidinone
                     5'-phosphate reductase"
                     /protein_id="HSD04947.1"
                     /translation="MGKSRPHVTISAAISLDGNIATKTGDSRLSSKKDIERVHKLRAS
                     VDAILIGKRTMMIDDPSLTVRYAKGKNPIRIILDSKGSIKSNSKIIQTCRNVPTIIAV
                     SEKTSQKNITRLQNHGLEILKCGKNKIDLKKLLQMLKKRNIKKLLVEGGGTTNWSFLK
                     ERLVDEIIITLTPFVIGGKNATSLVEGDGFDKISQACSLKLKKIYKMKNEIVLYYAI"
     gene            complement(10590..11099)
                     /locus_tag="VLB45_04260"
     CDS             complement(10590..11099)
                     /locus_tag="VLB45_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008300126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription factor"
                     /protein_id="HSD04948.1"
                     /translation="MTPSHEDPFVKIAALIGGDEYLKVARSLLNTEDATDEEIASSTG
                     LRINIVRKVLYDLFGKALITGIRVKDEKKGWFVYRWRARKDEVDNFIENQKKKITERL
                     QQRLDYEGSAEFYHCGNEDCSKLTFEKALDLSFKCPTCGKLVNITKNDKLKKALQYKI
                     DELREDLKR"
     gene            complement(11127..11654)
                     /locus_tag="VLB45_04265"
     CDS             complement(11127..11654)
                     /locus_tag="VLB45_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(56)-2'-O)-methyltransferase"
                     /protein_id="HSD04949.1"
                     /translation="MKIEVLRIGQRLVRDDRVTTHVALVSRAFGASRILMDEVNPEIK
                     KTISQVNKTWGGNFEVEIIENWKKILKLKKDSFKIVHLTMYGEDIDSVIDNIRKEEQI
                     LVVVGAEKVPREVYDIADYNVSVGNQPHSEISALAVLLDRVLQGKQFLKKHKDAQKEI
                     VPTKRGKKVLDKTID"
     gene            complement(11644..12771)
                     /gene="hflX"
                     /locus_tag="VLB45_04270"
     CDS             complement(11644..12771)
                     /gene="hflX"
                     /locus_tag="VLB45_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HSD04950.1"
                     /translation="MKDSAILITYDREDIIKEAISLCETAGYQVFKIIKQKFLNKAKF
                     GLGEGKVEELKEIIQTVKPDVIVFDEMLKPSQNYNLASTLKTNILDREALILEIFDRN
                     ATSSESKLQVKMAQLRYEMSRAKEKVRLAKMGEQPGFMGIGKFEVDVYYNDIKNRMTS
                     IKSKLVKAGKQRALHRQGRDRIGFKTISLAGYTSAGKSTLFNALTGEKQEESATLFTT
                     LSTTTRRIKIAETDVLVSDTVGFISRLPAYMVEAFRSTLEELVYADAVILVIDVSDPI
                     PELHKKFRSCMRTLEELGVEYDKIVFALNKSDLALPDEIIEKTTEIGLKESKKWLPIS
                     AITKQNIPQLKELLFAILESKPAITEKSKPRQTEFEIEHED"
     gene            complement(12797..13840)
                     /locus_tag="VLB45_04275"
     CDS             complement(12797..13840)
                     /locus_tag="VLB45_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010192076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate uptake regulator PhoU"
                     /protein_id="HSD04951.1"
                     /translation="MTVNEEMRKIQFTGKSSYIVSLPKEWVLDMGLKQGDQVTVVRQG
                     TSTLQIIPTKRHTKTTDTEDATFEIQPNDESASIVRKLVSLYFLGFRTINVKPKSGRL
                     TPSQRNAIKDAIKKVLMGTEVIADSTDGITIQVLINLFELSVDGAFKRMLLIAKSMQN
                     DALLALEEANFDLAKEVINSDDEVDRFSFYIIRQLKIAIQNEHMLKDMGFENARSCLG
                     YRVIVKNIERVGDHAVGIAKDLLEYKKPIKKTVLEAIREMNNFSLSVLDAACLSLFKK
                     DFAQAEAAIQKTSEITKYEKKVLDSIKSLRDDEEIYRVRRMIENIRRISEYASDIAEI
                     VMNMTIEKTLRKQ"
     gene            13945..14403
                     /locus_tag="VLB45_04280"
     CDS             13945..14403
                     /locus_tag="VLB45_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PUA domain-containing protein"
                     /protein_id="HSD04952.1"
                     /translation="MDSILKIKYTLDALFGNGVSKHLPKEIKFTYSKRTGRIQHMFQN
                     ETLLCTLRIDGGLAITPYFAQILLKNKKFKENCVEVDADSKPFVEKGSSVFCKHVVWC
                     GKNILIGADVPVLYEDTVIAVGRAVLSSRMIKSLKRGVAVKIRDSLKSTG"
     gene            14417..14758
                     /locus_tag="VLB45_04285"
     CDS             14417..14758
                     /locus_tag="VLB45_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016940006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nascent polypeptide-associated complex protein"
                     /protein_id="HSD04953.1"
                     /translation="MMRGGNREMRRMLDRMGLEMKEIPNVQEVIIKTDKKEIIIPKPA
                     VSEMKSKDNSIFQVIAESYEERELEVPVFSEEDIMLVAQQAGVSQEAANNALIEAKGD
                     LARAILLLTTK"
     gene            14794..15126
                     /locus_tag="VLB45_04290"
     CDS             14794..15126
                     /locus_tag="VLB45_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04954.1"
                     /translation="MSEVAVSKVEGIIKTLSELEENIDSLNEKVADMKKKLHAKAENE
                     IESLREKVVEMATKESEAIISKTRETANQEAKKIQSDGEKVLAEIKTKIDAKSDESVE
                     YVVSFILK"
     gene            15257..15820
                     /locus_tag="VLB45_04295"
     CDS             15257..15820
                     /locus_tag="VLB45_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04955.1"
                     /translation="MNYDSILPEEIVHSNNKLILTTMREAHNITNNLVLYDEEFDADP
                     TVIKGLITQKLESGMHENSLVIGIDPGNRIGLSVFYLQKEIESSIFTSIDELVSHVVK
                     ILAGLKADRKIIKIGNGNMRIAREITNLLNLSFCSHFELEFVDERKTSPKIKNYNKRG
                     EIDKLSAKYITQREGYRHLILPLSRVG"
     gene            16090..16440
                     /locus_tag="VLB45_04300"
     CDS             16090..16440
                     /locus_tag="VLB45_04300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04956.1"
                     /translation="MTCKGICTRYKAQKPVGTGRYASGQKRCQICEIFIKWEGLWCPC
                     CGYRLRTKPRNLKYKAKLRARVEADSQLEAVAVKAAEVEEIEVEPIAVKKATKKAKTA
                     KTLKKVAEPVAIKA"
     gene            16458..16793
                     /locus_tag="VLB45_04305"
     CDS             16458..16793
                     /locus_tag="VLB45_04305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04957.1"
                     /translation="MSLVGYKKKTVQVDNREFLVQMVPFDNGNFISLTEGKEKIGAMV
                     VSIGSGPSASTVTVIPSKSNSLFLKMVSERVASATNGICIVSINTSKELELEAMKVIM
                     NEIMEIVKQ"
     gene            16790..>17570
                     /locus_tag="VLB45_04310"
     CDS             16790..>17570
                     /locus_tag="VLB45_04310"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HSD04958.1"
                     /translation="MTELRAYIDQIKKLGQLHIIKKKVSTKYEIAALTAKVDGSNAIL
                     FENIKESKLRLVANLVGTRTRFAKAVNATESGIHDKVISAIEHAKKPKTISEGKFMEN
                     RSKDLSVLPIVTHFEKEPGPFITSSIIYTLNQEKNTQNSSFHRLLPLDRRHFSVRMVE
                     GRHLHRSFMYAKEHGEDLKVAITIGVHPAVSIAGAYQAEWGEDELEIANTLLENKMTL
                     AKTPYSRMKVPSNAEIVMEGKILHDKTHKEWMVEMLRTYDFK"
BASE COUNT         6229 a         2996 c         3158 g         5187 t
ORIGIN      
        1 agtgtaagct ttattgaata aaaaaattct tgttggtgtc atagctgcag gtgtaatagc
       61 tgttgttatt atgacagcat tcttatcaac acaaaacact atgttgcaaa aaaatgaaaa
      121 acttggttta tacatactta ctccatcatc tacgccgacc ctttcagatc taaaagaagt
      181 atatacagaa gctgcatctg ctggaatcgg tagaagtaat gtctatcttt tctggcctac
      241 aatagaacca caacaaggac agtatgattg gaaaattccc gacgctctta tgagtctcaa
      301 caaacaaaac gatcttaaag ttacattata tttttcaata atcaatagtc gtgtttttgg
      361 tccttttcca acttggatgg gggagccaca actagatgag agtctaaagg aaaaaaccgt
      421 taaaattctt gatgacattt tatcacgata tgatattgtt gatcatgtga taataggtgg
      481 agaaatagat gcatacttcc gtgaaaatga caaagacatt acaaaatatg aagaattttt
      541 cacaggcgta tatgatgatc ttaaagaaag atatccaaac gtaaaatttg gaaattcttt
      601 ttcacttcac ggtgtgctta atcaccaaca agaagacctt gttaccaagt taaatcaagg
      661 agattttgtt gcttttacat atttcccagt gaatacgcta aatgaaatag acaaaactcc
      721 tgaagacgca ggcaaaaatc ttgaaaaaat attagatctt gcaggtaaca aacagattgc
      781 tttgatggaa gtaagctggt ctactgcaga atccgttggt ggaagtaatg aaggacaggt
      841 aaaattcatg aaaatagtat atgatttcta tagaaaaaac gaaccacgct ttgaatttct
      901 ttcatggttc agacagtatg atagaccgat tgatagttgt atgaaaggtc ttaacacaag
      961 ctttgatatt agttttggaa acgagtttgt gttgcaacgc actgcagaat atgtatgtgc
     1021 tgcaggatta attgatgttg ataagcatcc aaaaccagct tgggaagaat tcaaaagaca
     1081 agtacaattc aatcccaatt cttaatatga tgtaaaaaga gattttgtac agttgagata
     1141 tctatctttt gttcttattg cattgttatt tgcaaatctt attcctgcat ttagtgattc
     1201 tctaccacct acaaaacaag agcaagatct atacaacaaa ctgcttgcag aatttgaaaa
     1261 actaaacata gaaaaaaaag atgtaagtga tgtactaaaa acatttgaaa aaccacttgt
     1321 agtaactttc ccatgtgatt ctactgacat taatgatcta gtagttagtg tagttgagac
     1381 aaaattagca agattcgatg aagcacaaga gcatgaaggc atgatcacag gtgggacata
     1441 cacatggaaa tgtacaatca caacaacatc tgatggcaag attacaatgg actgtgataa
     1501 cgaattaaaa ctaaatcatg atcttatgac ccaagacact ggaaaagatt ccagaataaa
     1561 acaagccgaa aacctgctca tattgtatca tgagctttta catggtcaac taatgataga
     1621 agccatacaa tcatctgaga aatggagaaa tgatgtatgc aataaatcca cagatggaaa
     1681 gattgactat tcatattctg acaaagatca taaaatcatt aatcctttgc agactgaatt
     1741 tctttctcag ttgattacaa attctggtgg aataatgatt gtacaagaaa tcatgccaga
     1801 agaaaccgag gatggcatat ttacaaaaaa attaggcaac ctacatgatt atcctcaata
     1861 cgtagaatct ggtatacatg taacactacg tggttcaaat cttgtagata ccgaattctc
     1921 ttctcaaaat tcagatatca ttttatctgg aagtctggca aacaaaacac aatctggtat
     1981 tgcatggctg tacatatttg gcaagccaga tgataaacag gaaacacaaa taatttctaa
     2041 aactgagata catattccag attgggttaa aaaaaatgct ggctggtggg cagctaacaa
     2101 aatttcagat tctgaattcc ttggtgcaat tgaatatctt atacaagaag gaataattca
     2161 agttccagaa actcaaaaac aacaggaatc taataagatt ccaaattggt ttaaacaaaa
     2221 tgctcaatgg tggtatgagg aaaaaataga tgacgagacg tttgcaaaag gaattcagta
     2281 tctcatatca gttggaatta tctctgtcta aactatagat gcggtcgccg ggatttgaac
     2341 ccgggtaacg ggcttggaag gccagtgtac taaccaaact atactacgac cgctcaacag
     2401 tgccaatctt tccactacat aataattctt ttttcgcatt cgtataatac ccgagaaatt
     2461 actacatgtg cttcaagtga attcttatca acagaaacaa gtctgttgat cctttctagt
     2521 attgcatcag agaagtgtcc ttttgaaaat ccaccaatta caatacatgt atctttgtca
     2581 agctctcgcg aaaacttttc gtatgaactc gtttctcctt ttatagacaa gccaactatt
     2641 tctttaggcc taatcttatc aatcaaatct acaaatttca tgtctttaag ttcaagtagt
     2701 tttttaccac cttcttctat ttttccttcc aagtatagtt tctcaattaa cccaacaaat
     2761 ctgtgatatg attttggtag ccgaacattt tctccaataa agataacttt gtcatcaatc
     2821 gtgtgaatat agacagctag tttattttca aagtataatg gaatggtaca acactctagt
     2881 agacaaaaat gaaccaagtc tggtctgccc cgttttattt catttttgat tcctttcatt
     2941 gctgcaaaat gccatgatat atctaacaaa acttcagacg gtcttttacc gactctcttg
     3001 gcatgtgcaa gtacagaatt gtgtttttgt agctctctag gaatcaattc tagtgcagat
     3061 tcagcaagca caagtgaaag caagatcgta ttttaaggga ctaacattat ttaactgaca
     3121 tgaagattgc cacaaggcaa atcatactgg aaagaatgga aattctgatt caaaatgctg
     3181 tctcaaacgc aagaaaaaat ccagaactag cacaaagaca agcaagtctt gcaaaaaaac
     3241 taagtacaaa acacagagtc agaatgccat atcaaatacg aatgaattat tgtaaaaaat
     3301 gtaagaaatt cattgctcct ggtgtaaacg caagaattcg acttggtcga tcttctgtaa
     3361 aatcaattcg tataacatgt agtttttgta atcatacata cagaaaaatt atttccaaag
     3421 ctcaatgatt tataagacga actccaaact ttcattttta tggctaaagc atatgatgta
     3481 ccagctgacg tattgattaa caaattatcg gagattctaa aaagcgaaga aatcacagct
     3541 ccagcatggg ctccatttgt taagactggt tcacatgcag ataagccacc acaaaaaaat
     3601 gattggtggt atacaagatg tgcatcactt ctaagaaaga tttaccttca tgggccagta
     3661 ggtgttaaag accttagagg aatgtacggt ggggcaatag cagttggata tggcggtgct
     3721 catcacagag atgctggtgg agcaataatc agaactgcta ttcatagttt agaaaaactt
     3781 ggttatattg acaaagtaga aggaaaagga agaacagttt cgcatcaagg aatgaaaaaa
     3841 atagatcgag ttgccacaga aattctaaat gaattaattg taaaaaatcc aaacctgaag
     3901 aaatactctt agtgtggttt tatgagtaac ccaaatcaag gtaatgagaa agaacctacc
     3961 gaatctgaag ttgcatcgat gaaagaacag gcacttcgca ctcttttgga accacaagct
     4021 agactacgac taaacaatgt aagaatggtt aaaccagaac ttgctgcaat ggttgaaaac
     4081 tatctcattg gtatggcatc acaagggaga ttaaacacac aaatttctga tgatcaacta
     4141 aaacaaattc tccagtcgat tcaacaacca aaacgtgatt ttaaaattaa tcgaagatag
     4201 atctagcgaa aatatattag gggtaaattg agcgtctgta cttatgaaag ccgtagtcta
     4261 cgaggcatat gcaccaaatg acgattacaa atcaatttta aaagtaaaag atattccaga
     4321 accaaaacca aaaccagatg aggtagtttt caaggtcaaa gctgctgctt tgaattataa
     4381 tgacatatgg ggaatgaggg gacaaccagt tgcagttcca ttaccgcatg tctctggttc
     4441 tgatgctgcc ggtgaggtta ttgcagttgg agaggatgtt acaaatttca aggtaggtga
     4501 tagagttgtt tcacactcaa acatgtcatg cagagtctgc gctgcatgca cagacggacg
     4561 agaatttgat tgtataaaaa gaacaatttg gggatttcaa acaggtccta catggggagc
     4621 tttttctgag ataacacatt tgccagaagt aaatgtggca aagattcctg aaggagtaag
     4681 ttttgatgaa gcagctgcag cttcgatgac tttgttaaca tcttggcata tgcttgtagg
     4741 cagagcaaag atacgaccag gtcaaactgt gcttatcatg ggtggtggct ctggagtagg
     4801 aagctttgga attcaaattg caaaacttta caattgtaca gtaattgcaa ctgcaagtcc
     4861 tgacaaacta gataaattaa aacaacttgg tgctgattat gttgtagatc acagaaaaga
     4921 agactggaac aaagaagttt ttaaaattac caaagagatc agtgctaaaa caggagtggc
     4981 tcctggtatt gatgtttcat ttgatcacat tggacaaaca cactggaatc ctcaacttac
     5041 cttactgaaa tatggtggaa cactagtttc atgtggtgca acaacaggat atgatgcaaa
     5101 aactgatttg cgacacatat ttttcaaggg aactaacata cttggttcta cacaaggaac
     5161 aaaagctgaa ctagaacaag gattgtactg gatggctaaa ggcaaaataa aagcagtgat
     5221 tgattctgta tacagctttg aaaatgccgc tgaagctcat acgaagatgt taactggaaa
     5281 aggcttgttc ggtaaaatct taatgaagcc caatgcctag tcaatttcat ggtcagactt
     5341 ctgcgtagtc ttatctttgt accaggaaac aattctagat ttttggaaaa agcaaaaact
     5401 ttgcctgctg atataatttg ttttgattta gaggattctg taccgataaa tgaaaaaaat
     5461 gtagcaagaa atttaattaa gaatgctttg acaaagcatt caaaatacaa agcagaaatc
     5521 tatgttagaa caaattcacc tgtatcggga atgattccag atgatctttc caaaataata
     5581 cagaagggga tcgatggcat agtcattccc aaagtaaata atgcaataga attaaaacga
     5641 atagaaaaac ttgttacaag tttagaaaaa aaacggaaac taaaaccaat agagatcatt
     5701 ccatctattg aatcagcaga aggagtagta aatacatatg acatagcatc atccagcaaa
     5761 agaattcctg ctgtagtctt tggtgtattt gatctactaa atgacatggg tatagaatac
     5821 acaaaacaac cggagggagc aaaatatgct agagcaaaga ttccaattga cacaagagcc
     5881 gctggaaaat atgcaataga tgcaatttgg caggatctaa atgatgaaaa tggacttgtg
     5941 aaagactgta aaataggaaa aaatcttggt tatgcaggaa aaagcatcat acacccaaat
     6001 caaatccaca ttgcacatag catattcaaa ccaaaccaag atgagataga ttgggccaaa
     6061 aaggtatgtc aggtatatgc taattcaata aaacaaagta aaggagctac gatagttgaa
     6121 ggaaaaatga ttgatgaggt tcattataaa cgagcaaaat cacttctaga ttcaataaaa
     6181 aatagctaaa accagtatag gcacaaaaag cactagagtt tcaaaataag taatttatag
     6241 actttaagat cattttacta tgtgtttaca gggattatag aaggtcttgg aataatcaag
     6301 aaatttgaga aaaaaacgaa aacaagaagc gctgcaaaaa tgaaagtaga cttgggtgat
     6361 cttgcaaaag gactcaaagt tggacacagt gttgcaataa atggagtatg tcttactgta
     6421 acaaaaattt ccaagaacca agcggaattt gagatgatta atgaaaccat aaagaaaacc
     6481 gatcttggag ctttggaatc gggagacaaa gtgaacattg agcgtagttt gaaagttggt
     6541 gacagaatgg aggggcattt tgttcttggt cacattgatg atacgggtaa aataataaaa
     6601 atagaaaaaa tgccaaaaga gatcaaactg tggattgaaa tttcaaaaga tctagcaaga
     6661 cacatagtaa aaaaaggatc tatcgcaatt gatggaataa gtctaacact tgttgatgta
     6721 ataaaaaata aaatctctgt ctgcatcata ccacatacaa tgaaaattac aaatcttaat
     6781 tcaaaaaaaa ttggtgataa agttaatatt gaaactgaca ttcttgggaa atatacaact
     6841 aagaaaatct aatatctacc acttttatgg tagtaaccaa ttttgtagta agctttataa
     6901 ttgttgaaaa gccgatttaa tacatgtcct tagatgtagc aatctcatca ctgcaaagag
     6961 gcgaatttgt tctgcttcat gatgcaggca agcgtgaaaa tgaggtagat atggtagttg
     7021 cagcagaatt tgttactcct gcacatatag caagaatgcg tcaatacgca ggtggactag
     7081 tgtgtctcgc aataaacgac tcttttgctc aaaacttagg attgaaatac atgcatgata
     7141 ttctttccaa ttctgatgac gttgacattg aatcaaaaaa aatgatcatg ggaaaagcac
     7201 catatggaga tcaaccatcc ttttcaattt caatcaatca cgctcaaaca tttacaggca
     7261 ttaccgatag agatagggct ttaacaataa aagaaatggc caatctctcc aaaactttca
     7321 atgaaaataa aaagtatgaa tttatttcat catttaaaac tccaggacat gtacctgtat
     7381 taatagcaaa aaatggatta ttatcaaaaa gattgggcca tacagaaatg tctgtatatt
     7441 taatggaact tgcaaaacta tctcctaccg cagcaatttg tgaaatgctt gactcagaat
     7501 catataccgc attatctgtg gaaaaggcta aaaaatatgc aaaagaaaat gcaattccat
     7561 ttcttgaagg aaaagatctc ttagaatttt ctaaagtgca ctagttatga atattgcaat
     7621 agtggtttcg gaatttaacc aagaaataac ttcaaaaatg tttgaagttg cacttgaaaa
     7681 ggcaaagaat ctaaaactaa acataaaata cacttgtcaa gttcctggtg tttttgatat
     7741 gccagttgta atagattcct tattgcaaaa aaaggacata gatgcagttg taactcttgg
     7801 tgccgtaatt aaaggacaga caaaacacga cgaactgatt gcaaacgtga ctgccaaaat
     7861 catagctgaa ctttccataa aatatcaaaa accagtaact ctgggaataa ctggaccagg
     7921 catgacagac aagcaggcac aggcacgaat aaggcctgtt gctgagagag ctgtagaggc
     7981 tacagaaaag atagcaaatc aattgagaaa gataagatga tccaacttac catactaaag
     8041 ataacaggtt atggtccatg gacacttaca cttggaagtg atagggaaca cagattacaa
     8101 attttgcaat cttctttgta taaagaaatt caaaatcttt tttcagaaaa aaactgtcta
     8161 gttttttcta atagatttga tgagctcttt gcaatcacaa atggtcttat tctaagtgat
     8221 catgttgaaa ttcaaaagaa actaaaagaa tcgtttgaac tcgatctctc aatgtcaata
     8281 ggatgtggtg aatctccttt tgatgcaaat ctgcaagcat atgaagcaag aaaatccaaa
     8341 atcttcttga atgaggaact taacatattt ggttttacag atggtaaaac agaacaaaat
     8401 gtgcaaataa tgcacattga tgtagatggt tctacttcga tatctacaaa aaaatcacca
     8461 tatgaaatct ctttacttat gttcaaactc tattcagaaa tgtcagaatt tttcttaaaa
     8521 caaaactatc tgtctttctt catgggcgga gataacttta tgattgttac ttctgggaat
     8581 ggacaagaga atgcatctaa gtttctagat ctgatagaaa aaaaatataa tataacaatg
     8641 aattgcggaa taggaaccgc aaagactgca cgggaagctg ctaaacttgc aacaaaatcc
     8701 cttgatacaa taagagaaat gagagattct ggaaaaacac aacgagtact tgaattaaca
     8761 tgctgataaa aaatgccagt gtactttatg gtaatgaatt aaactacatc caaaacacaa
     8821 acatcagaat ttctgataat ctattttcta aaataggcac gaatctaaag ccattgacaa
     8881 aagaagagac tcatgattgt gaagggctgc tcatgattcc tggatttatt aatgctcata
     8941 ctcacatagg agattcaata gcaaaagata ttggcataga ctatggaata gaagaaaaaa
     9001 ttcatccagt gatggggttc aaacataaaa ttctcaaaaa tactgaatca tcacatctga
     9061 caagtttcat gagaaattcc tgcatttcca tgatgaaaaa gggcattacg acttttattg
     9121 attttaggga ggggggtctt gatggaatat tgattttaaa aaaagcaatt tctaaccttc
     9181 ccatacgatc tataattcta ggaagaattg attattatca aaacacatca tcaattaaaa
     9241 aaaacattcc tctatcagca tcaaaaaaga aagagcttac aaaactttta gaaaaatgtg
     9301 atggagttgg tataagtgga cctaatgaaa atagtaactc tgtattaagg cattattcac
     9361 aaacaaaaaa gatcaaagca attcatgcag ctgaaacaat aggaagctgt gaaatttcaa
     9421 aaaaatccac cggaaaaaca gagacagagc gagccatgat tctaaaacca aattttctag
     9481 tacacatgac acatgcatca aaacatgatc ttgttcttgc tgcacaaaat aagatcagta
     9541 tagtagtatg tccaagagca aatgcatctc ttgcagacgg aattccaaat gtacacttga
     9601 tgatgaaagc tggatgtaat ctagctatag gtacagacaa cgtcatgata aactcgcctg
     9661 atatgtttag ggaaatggat tatctgtgga agatctcaat gggaatgcaa aaaactaggc
     9721 tttccccaaa gtctatactc aaaatggctt cagtaaacgt ctcaaagatg cttaaaaata
     9781 agcaattggg aaccattagt ccacaaagct ttgctgactg cattttcatc gataaacacg
     9841 caatagactt ggaaccaatg cataatccac atgcatccat tgtacataga gcatctgaaa
     9901 gcacaatcaa ggcagtaatg attgggggga gaatagttca tgggaaaatc tagaccacat
     9961 gttaccatta gcgccgctat cagtcttgat ggaaacattg caaccaaaac gggggattcc
    10021 agactctctt caaaaaaaga cattgaaaga gtgcacaaac ttagagcaag tgttgatgca
    10081 atacttattg gaaaacgtac aatgatgata gatgatccct cacttacagt aagatatgca
    10141 aaaggaaaaa atcctatcag aattatttta gattcaaaag gttctataaa atcaaactca
    10201 aaaataattc agacctgcag aaatgttcct accattattg cagtatcaga aaaaacatct
    10261 caaaaaaaca taactcgatt acaaaatcat ggtcttgaga tactgaaatg tggcaaaaac
    10321 aaaattgatc taaaaaaatt attacaaatg ctcaaaaaaa gaaacataaa gaagcttctt
    10381 gttgaaggtg gcggtactac taactggtct tttctcaaag aaagacttgt agacgagatt
    10441 ataatcacat tgacgccatt tgttattgga ggaaaaaatg cgacttctct agtggaaggc
    10501 gatggttttg ataaaatctc tcaagcatgt tctttgaaac taaagaaaat ctataagatg
    10561 aaaaatgaga tagtgttata ctacgctatc taacgtttca aatcttcccg taattcatca
    10621 attttgtact gtaacgcttt ctttaacttg tcatttttgg ttatgttaac aagctttcca
    10681 caagttggac atttgaagga caaatccaag gctttttcaa atgtaagctt tgagcagtct
    10741 tcatttccac aatgataaaa ctctgcagag ccctcataat ctagtctttg ttgtagtctt
    10801 tctgtaatct tctttttttg attttctata aaattgtcga cctcatcctt tcttgcacgc
    10861 catctgtaaa caaaccagcc cttcttttca tctttcactc taataccagt gatcagtgct
    10921 tttccaaata gatcatacaa aacttttctt actatgttaa ttctaagacc ggttgaactt
    10981 gcaatctctt catcagtggc atcttctgta tttaaaagtg aacgagcaac tttgagatat
    11041 tcgtctccac caatcaaagc tgctattttt acaaaaggat cttcgtggct gggtgtcatc
    11101 agcgtccttt ggtttggatt ctatatttaa tcaattgttt tgtctaacac tttttttcct
    11161 ctttttgtag gaacaatttc tttttgtgca tctttgtgtt ttttaagaaa ttgttttccc
    11221 tgcaggaccc tatccaataa aactgccaag gcacttatct cagagtgagg ttggttgcca
    11281 actgatacat tatagtctgc aatgtcataa acttcacgag ggactttttc tgctccaaca
    11341 acaacaagaa tttgttcttc ttttcttatg ttatctatga ctgaatcaat atcttcacca
    11401 tacattgtaa gatgaacaat tttgaatgaa tcttttttta atttgagaat ttttttccag
    11461 ttttcaataa tctcaacttc aaagttacct ccccatgtct tatttacttg agaaatagtc
    11521 tttttgattt cgggattgac ttcatccata agtattctag atgctccaaa agctcttgaa
    11581 acaagagcca catgtgttgt tactctgtca tctcgtacta gtctttgacc tattctaaga
    11641 acttcaatct tcatgctcta tttcaaattc agtctgacgt ggtttgcttt tttctgtaat
    11701 tgctggtttg ctttctaaaa ttgcaaataa gagttccttt aactgtggaa tgttttgttt
    11761 tgttattgca gatatcggga gccacttttt gctttctttt aatccaatct ctgtcgtctt
    11821 ttcaataatt tcatctggta atgcaagatc tgatttgttt aatgcaaaaa ctattttgtc
    11881 atattcgacc ccaagctcct ctaatgtacg catacaactt ctgaatttct tatgtagttc
    11941 aggtatggga tcactaacat ctattacaag tatcactgca tctgcataga caagctcttc
    12001 aagcgtagat ctgaatgcct caaccatgta cgcaggaagc ctactgatga atcctacagt
    12061 atcagaaact aacacatctg tttcagctat tttgattctt ctcgttgttg tagaaagagt
    12121 tgtgaaaagc gttgcgcttt cttcttgttt ttctcccgta agtgcattaa agagagttga
    12181 tttaccagct gatgtgtaac ctgccaacga aatagttttg aatccgatcc tgtctctgcc
    12241 ctgtctgtgt aacgcacgtt gttttccagc ttttacaagc ttggatttga tactggtcat
    12301 cctgtttttg atgtcattgt agtacacatc tacttcaaat tttccaatac ccatgaatcc
    12361 tggctgttca cccatttttg ctaatctgac tttttctttt gcgcgtgaca tttcatacct
    12421 taactgtgcc atcttgactt gtaattttga ttcagaactt gttgcgtttc gatcaaatat
    12481 ttctagaatc aaagcttctc tatctaaaat gttagtcttt aatgtagatg caaggttgta
    12541 gttttgacta ggctttagca tttcatcaaa aactattaca tctggtttta ccgtctgtat
    12601 aatttctttt aattcttcca cttttccttc tccaagacca aactttgctt tgtttagaaa
    12661 tttttgtttg ataattttga agacttgata accagccgtt tcacaaagtg aaatagcttc
    12721 ttttatgata tcttctctat cataggtaat caagattgca gaatctttca ttatattgaa
    12781 tgcaatcact cgtatcttat tgctttcgca gagttttttc tatagtcata ttcatgacaa
    12841 tttctgcaat gtcgcttgca tattctgaaa ttctccgtat attttctatc atccttctta
    12901 cacgatagat ctcttcatca tctcttagag atttgatgct gtctagtact tttttctcat
    12961 attttgtaat ttctgaggtc ttttgtatgg ctgcttccgc ttgtgcaaag tccttcttga
    13021 atagagacag acatgcagca tcaagaacag atagggaaaa gttgttcatt tcacgaattg
    13081 cttcaagaac agtttttttg attggttttt tgtattccaa taggtctttt gctattccaa
    13141 ctgcatgatc cccaaccctc tctatgtttt tcacaatgac tctatagcca agacaactac
    13201 gtgcattttc aaaacccatg tcctttaaca tatgttcatt ttggattgca atttttaatt
    13261 gtctaattat gtagaagcta aacctatcta cctcatcatc actgtttatg acttcctttg
    13321 caagatcaaa gtttgcttcc tcaagagcaa gaagcgcatc attttgcata gattttgcaa
    13381 taagaagcat tcttttgaat gccccgtcta ctgaaagctc aaatagattt atcagaactt
    13441 gaattgttat gccgtctgta gaatctgcaa ttacttcagt tcccattagg acttttttta
    13501 tggcatcttt aatggcattt ctctgagatg gagttaatct acctgatttt ggttttacat
    13561 ttattgttct aaatccaaga aaataaagtg aaacaagttt tcttacaata cttgcacttt
    13621 catcatttgg ttgtatttcg aaggttgcat cttcagtatc agtagtcttt gtatgacgct
    13681 ttgttggaat gatttgtaat gtagatgttc cttgtcgtac cactgtgact tgatctccct
    13741 gtttcaaacc catatccaac acccactctt ttggtaatga aacaatatag gaagattttc
    13801 cagtaaattg gatctttctc atttcttcgt taactgtcat gactagactt tcttatgcca
    13861 ttctatatag ttattctgaa atgtatatag tataatctta aatccaaaaa acaattagaa
    13921 ccctttacag tctatattct attcatggat tcaattctaa agatcaagta cactcttgat
    13981 gcactctttg gtaatggtgt ttctaagcat ctcccaaaag aaatcaaatt tacatactca
    14041 aagagaacag gcagaatcca acacatgttt caaaatgaaa cccttctttg tacactacga
    14101 attgatggtg gccttgcaat aacaccatat tttgcacaga tcttactaaa aaacaaaaaa
    14161 ttcaaagaaa attgtgttga agtagatgct gactcgaaac cgtttgtaga aaaaggaagc
    14221 tcagtttttt gtaagcatgt tgtatggtgt ggaaaaaaca ttttgatcgg tgctgatgta
    14281 cctgtcttat acgaagatac ggtgattgca gttggaagag cagtactatc ctcacgaatg
    14341 atcaagtcac taaaacgtgg tgtggctgta aaaatcagag atagtttaaa aagtacaggt
    14401 tgatcaagtg aatcatatga tgcgtggcgg aaaccgagaa atgagaagaa tgctagaccg
    14461 aatgggtcta gagatgaaag agatccctaa tgtgcaggaa gtaataatca agactgataa
    14521 aaaagagatt atcatcccaa aacctgctgt gtcagaaatg aagtcaaaag acaattctat
    14581 ttttcaggta attgcagaaa gctatgaaga aagggaatta gaagtaccag tgtttagcga
    14641 agaagacata atgttggttg cacaacaggc cggcgtttcc caagaggcag caaacaacgc
    14701 tctgatagaa gcaaaaggag atctagcacg ggcaattttg ttacttacaa ccaaatgaag
    14761 gatttaataa caggattttg aggttttagc ctgatgagtg aagtcgcagt ttctaaggtt
    14821 gaagggataa tcaagactct ctctgaacta gaggaaaata tagactctct caatgaaaaa
    14881 gtagccgata tgaaaaagaa actacacgca aaggcagaaa atgagattga atctttacga
    14941 gaaaaggtag tagaaatggc tacaaaagag tcagaagcta taatatctaa aactagagaa
    15001 acagcaaacc aagaagccaa gaagattcaa agtgatggtg aaaaagtgct ggctgaaatt
    15061 aaaaccaaga ttgatgcaaa atctgatgaa tctgtagaat acgtagtttc ttttatactg
    15121 aaataggcat aaaaccttta aattttgctt taaactaaat caaaatatcg cataccatac
    15181 acaaaacaac attgttgtag cgactgttta tggacgacca tatttcaaat ttgtaaaaac
    15241 acttcgattc ttaaaaatga attatgattc tatattgcca gaagaaatag ttcattccaa
    15301 caataaactc attcttacaa ccatgcgtga agcacataac ataaccaata atctagtgct
    15361 ttacgacgaa gagtttgacg ctgatccaac agtaatcaaa ggactgatca cacaaaaact
    15421 agagtcagga atgcatgaaa actcacttgt gattggaata gatcctggca acagaatagg
    15481 tctttccgta ttctatcttc aaaaagaaat tgaaagttca attttcacat caattgatga
    15541 actggtatcg catgttgtga aaattctggc aggattaaag gctgatagaa agatcataaa
    15601 aattggaaat ggaaacatgc gaattgcacg agagatcacc aatcttttga atctaagttt
    15661 ttgttcacat tttgaactag aatttgtaga cgaacgcaaa acttcaccca agataaaaaa
    15721 ttataacaaa aggggagaaa tagacaaact gtctgcaaaa tacataacac aaagagaagg
    15781 ttacagacac ctgattctgc cactatccag ggtaggctag atgaattttg atagaaaaca
    15841 acaaatcgtg aaaaaatctt tattagttgg cattcgattt tggctaaaaa tttctaaatt
    15901 ctagattttt agaaaacata agtttttata tagctttttt cgttttttct gaaaactctg
    15961 gaatggttca agaaggacct gtataatttt tacataaaat catcattatc aatatagaaa
    16021 attacgatcg atccttaaat acttagtaat actataacat attacaaagg agaatagtga
    16081 acagaaaaaa tgacatgtaa aggaatatgc acaagataca aggcacaaaa accagttggt
    16141 actggtcgct atgcatcagg acagaaaaga tgccagattt gtgaaatttt cataaaatgg
    16201 gaaggactct ggtgtccatg ctgtggatac agactgcgaa caaagccaag aaacctaaaa
    16261 tacaaggcaa aattacgcgc tagagttgaa gctgattctc aactagaagc tgttgctgta
    16321 aaggccgcgg aagtagaaga gatagaggta gaaccaatag cagtaaaaaa ggctacaaaa
    16381 aaggctaaga cggcaaagac tttgaaaaag gtagctgagc cggtagcaat aaaggcgtag
    16441 ctaacgttag accttcaatg tccctagtcg gttacaagaa aaaaaccgtc caagtggata
    16501 atagagagtt cttggttcaa atggttccat ttgataatgg taattttatt tcattaactg
    16561 aaggtaaaga aaaaattggt gcaatggttg tatcaatcgg ttctggtcct agtgcaagta
    16621 ctgtaacggt tataccgtca aaatccaatt cccttttttt gaaaatggtt tcagaacgtg
    16681 ttgcatcagc cacaaatggc atctgtattg tttctattaa tacatcaaaa gaacttgaac
    16741 ttgaagccat gaaagttata atgaatgaaa taatggaaat tgtaaaacaa tgactgaact
    16801 cagggcatac attgatcaga taaaaaaact tggtcaactg cacatcataa aaaagaaggt
    16861 ttcaacaaaa tacgaaattg cagctcttac tgctaaagta gacggttcta atgcaatttt
    16921 gtttgaaaat ataaaagaaa gcaagcttcg acttgtagca aatttggtcg gtactagaac
    16981 cagatttgca aaagcggtga atgcaaccga gtctggtatt catgataagg taatttctgc
    17041 aattgaacat gcaaaaaaac caaagacgat ttctgaagga aaattcatgg aaaacaggtc
    17101 aaaggatctg tcagttttgc ccattgtaac gcattttgag aaagaaccag gcccattcat
    17161 cacctcttct atcatatata cactaaacca agaaaaaaac acacagaact cctcatttca
    17221 caggcttttg cctctagata gacggcattt ctctgtacga atggtagaag gaagacacct
    17281 gcatcgctct tttatgtatg ccaaagagca cggcgaagat ctcaaagtgg caattacgat
    17341 aggagtacat ccagctgtgt caatagctgg tgcataccaa gcagagtggg gagaagatga
    17401 gctagaaatt gcaaatactc tattggaaaa caaaatgacg ttagcaaaaa caccatactc
    17461 tagaatgaaa gtgccctcaa atgcagaaat tgttatggaa ggaaaaattc tacatgataa
    17521 aactcacaag gaatggatgg ttgaaatgct cagaacatat gatttcaaaa
//
LOCUS       DATIDL010000098         3752 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrosopumilaceae archaeon isolate SMAG_U16342
            k141_1935991, whole genome shotgun sequence.
ACCESSION   DATIDL010000098 DATIDL010000000
VERSION     DATIDL010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935836
            Sequence Read Archive: SRR25158326
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosopumilaceae archaeon (soil metagenome)
  ORGANISM  Nitrosopumilaceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrosopumilales;
            Nitrosopumilaceae.
REFERENCE   1  (bases 1 to 3752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/04/2024 05:09:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,520
            CDSs (total)                 :: 1,474
            Genes (coding)               :: 1,474
            CDSs (with protein)          :: 1,474
            Genes (RNA)                  :: 46
            rRNAs                        :: 1 (5S)
            complete rRNAs               :: 1 (5S)
            tRNAs                        :: 43
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3752
                     /organism="Nitrosopumilaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1935991"
                     /isolate="SMAG_U16342"
                     /isolation_source="soil"
                     /db_xref="taxon:2202732"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..772
                     /locus_tag="VLB45_04315"
     CDS             <1..772
                     /locus_tag="VLB45_04315"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HSD04959.1"
                     /translation="EGFKATGGFNDEVDVTYSLILSIVAAKKLGKSFSEVIFDVIKNS
                     DQTGIGSVEKYLDTLQIDLSEIRKKLSYPGIHDTNILYSIFDEIFYGDELYYQLYKKK
                     PEFFSGKGLIDNDVVLLKKELIDALRKKFGKKIAIVSGRGYISTKYSLKKLFEEFDIK
                     NSRFLEDEPREMAKPNPKSLVSSIKDLHSSCTMFVGDSMEDYIMTRRSEELGNKTVFC
                     GIYGTSKDPVSKRNLFEQKNADIILESIDLIPKTLNLV"
     gene            802..1389
                     /gene="hisB"
                     /locus_tag="VLB45_04320"
     CDS             802..1389
                     /gene="hisB"
                     /locus_tag="VLB45_04320"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HSD04960.1"
                     /translation="MKSRVSRINRKTKETEVSVNVNLDGNGKVSVKTGISFLDHMITS
                     LAKHSMLDLKLNAVSKDGITHHLIEDTGIALANAMDLSLGNRSGIVRFGHASVPMDES
                     LAEASLDLVKRPYHKIDLVIERKEIEGISKEDLEHFFQSFAQNLNICMHITVKYGKND
                     HHKIEAALKAFAVAWRAAAGPDKKQKGIPSTKGAM"
     gene            1389..1994
                     /gene="hisH"
                     /locus_tag="VLB45_04325"
     CDS             1389..1994
                     /gene="hisH"
                     /locus_tag="VLB45_04325"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007551406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HSD04961.1"
                     /translation="MVKVAIFDYGAGNIFSLKSSLEKNGAEVDVITDLNHGSIYSGLL
                     LPGVGNFDPAITSITSSKKAFVDIVNNQIPVLGICLGMEMFFEKSEEGTENGLGVLEG
                     EVILLPNKFKIPHMGWNDLRIKKPSKLLEGVKEGSWVYFVHSYRAKPKNAEIVKADSD
                     YGISVPAVIESGTLFGTQFHPEKSGKIGSVMINNFLRECKK"
     gene            1991..2698
                     /gene="hisA"
                     /locus_tag="VLB45_04330"
     CDS             1991..2698
                     /gene="hisA"
                     /locus_tag="VLB45_04330"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HSD04962.1"
                     /translation="MKVIPAIDLMDGKVVRLVKGDPKNKTVYSDDPVETAKKWEKAGA
                     DRLHVVDLDATLGGGSNLQVIKKIAKEVSIPIHAAGGLRDEKIISEVLSFADKVVIGT
                     FAFKNRDDLPKIMQKFGKEQIIISVDQLNGKIVINGWKQDTGIDLISGIKDFLKIGLS
                     EFLLTSVDRDGTLQGPDIDSLSKACKIKNTMIIASGGISNIDDIVAVKKCGATGVILG
                     KALYDGKISIEKVRAIA"
     gene            2695..3498
                     /gene="hisF"
                     /locus_tag="VLB45_04335"
     CDS             2695..3498
                     /gene="hisF"
                     /locus_tag="VLB45_04335"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HSD04963.1"
                     /translation="MTLTKRVIPCLDVDNGRVVKGMHFASIKDAGDPVVLAEKYSKEG
                     ADELVFLDITASEQGRETIKALVSKVAQVIDIPFTVGGGVKTLQHARDILLSGADKVA
                     INTGAVKNPEILTKLMKIFGKQCVVVAMDVKRNYDFEKGKHVFSADSKKFWFEVYIYG
                     GKTATGLDAIEWAKEVERLGAGEILLTSIDMDGTKEGYDIQLTKEIVNAVNIPVIASG
                     GCGKPVHMLDVFLQSNVDAALAASIFHYKTHSVDRVKEYLKENGVPVRI"
     gene            3535..>3752
                     /locus_tag="VLB45_04340"
     CDS             3535..>3752
                     /locus_tag="VLB45_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007403044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HSD04964.1"
                     /translation="MQKTIDDIDFSKGDGLVPVIVQDIETKEVLTLAYANKESIALTQ
                     KTGNSWFWSRSRNKLWMKGEESGNTQKV"
BASE COUNT         1339 a          599 c          813 g         1001 t
ORIGIN      
        1 tgaaggcttc aaggcaacag gtggctttaa tgacgaagtt gatgtcacat attcattaat
       61 actttcaatt gttgctgcaa aaaaacttgg caagtctttt tctgaagtca tttttgatgt
      121 aataaaaaac tctgatcaaa caggaatagg atcagtagaa aaatatcttg acacactaca
      181 aatagatctt tctgaaatac gaaaaaagtt gtcctatcct ggtatccatg acacaaatat
      241 tttgtattct atatttgatg aaatatttta cggggatgaa ttgtattatc aattatacaa
      301 aaaaaagcca gaattttttt ctggaaaagg actgattgat aatgatgtgg tactcctaaa
      361 aaaagaatta atagatgcac tacgaaaaaa atttggcaaa aaaattgcca tagtttctgg
      421 gcgtggctat atttctacca aatattcact aaagaaatta tttgaggaat ttgatatcaa
      481 aaattcgcga ttcctagaag acgaaccacg tgagatggcc aagccaaacc caaaatctct
      541 tgtttcatcc ataaaagatc tacattcttc atgtaccatg tttgtgggag attctatgga
      601 ggactatatc atgacaagaa gatcagaaga gcttggaaac aaaactgtct tttgtgggat
      661 ttatggcaca agtaaggatc ctgtctcaaa acgtaatctc tttgagcaaa aaaatgctga
      721 tattatactg gaatcaatag acttgattcc gaagacatta aatctggtct aggcataatc
      781 tcgttctctg caggaattta tatgaaatct agagtgagcc gcatcaatag aaaaacaaag
      841 gaaaccgaag tttctgtaaa tgtaaatctg gatggaaacg gaaaagtttc tgtaaaaaca
      901 ggaataagct ttcttgatca catgataacg agtcttgcta aacactctat gcttgatcta
      961 aaattgaatg cagtttcaaa agatggaatt acgcaccacc taattgagga cactggaatt
     1021 gcactagcca atgcaatgga tttgtcactt ggaaaccgat ctggaattgt aagatttgga
     1081 catgcatctg tgccgatgga tgaatcacta gcagaagcat cacttgatct agtaaagcgt
     1141 ccgtatcaca aaatcgatct agtaattgaa cgaaaggaaa tagagggaat ctcaaaagaa
     1201 gatctggaac acttttttca atcctttgct cagaatctca acatctgcat gcacatcaca
     1261 gtaaagtatg gcaaaaatga tcaccacaaa attgaggctg cactaaaggc ctttgcggta
     1321 gcatggcgag cggcagctgg tcctgacaag aaacaaaaag gaataccaag tacaaaaggt
     1381 gcaatgtagt ggttaaagtt gcaatatttg attacggtgc aggaaacatt ttcagcttaa
     1441 agtcttcatt ggaaaaaaat ggagccgaag tggatgtcat aacagatcta aaccatggta
     1501 gtatctattc aggattgtta cttcctggag ttggaaactt tgatcctgca attacaagca
     1561 taacgtcatc aaaaaaagca tttgttgata tcgttaacaa ccagattcct gtgcttggca
     1621 tctgccttgg gatggaaatg ttctttgaaa aaagtgagga gggaacagaa aacggacttg
     1681 gagtcttgga aggcgaggta atactgcttc caaacaagtt caagattcca cacatgggat
     1741 ggaatgattt gagaataaaa aaaccaagca aacttttgga aggggtaaaa gaaggctcat
     1801 gggtgtactt tgtgcactcc tatagagcaa aaccaaaaaa tgcagaaatc gtaaaggcag
     1861 attctgatta tgggataagc gttccagcag taatagaaag tggcactctt tttggaacac
     1921 aatttcaccc agaaaaatct ggcaaaatag gctctgtaat gatcaataac tttttgcgag
     1981 agtgcaaaaa gtgaaagtca ttcctgcaat tgacctcatg gatggaaagg tagtacgact
     2041 tgtaaaaggg gatcctaaaa acaagacagt ctatagtgat gatcctgtag agactgcaaa
     2101 aaaatgggaa aaagcgggag cagacaggtt acatgttgta gatcttgacg caaccttggg
     2161 gggtggttca aatttacaag taattaaaaa aattgcaaag gaagtgtcta tcccaataca
     2221 tgcagctggt ggattacgtg atgaaaaaat aatatctgag gtgctttcgt ttgcagacaa
     2281 ggtggttatt ggtacatttg catttaaaaa cagagatgac ctgccaaaaa ttatgcaaaa
     2341 atttggaaaa gagcaaataa taatttctgt agatcaacta aatggaaaaa ttgtcataaa
     2401 tggatggaaa caagatactg gaatagatct gatttctgga atcaaagatt ttctaaaaat
     2461 tggtctatct gagtttcttt tgacaagtgt tgatagggat ggaactctgc aaggaccaga
     2521 cattgattcc ctaagcaagg catgcaagat aaaaaataca atgataattg caagtggcgg
     2581 tatatccaac atagatgata ttgttgctgt aaaaaaatgt ggtgcgacgg gagttattct
     2641 cggaaaagca ctatatgatg gaaaaatttc aattgagaag gttagggcaa tagcatgact
     2701 ttaaccaaaa gagtgatccc ttgtctggat gttgataatg gtagagtagt caagggaatg
     2761 cactttgcat caatcaaaga cgcaggtgat cctgttgtgc ttgctgaaaa atacagcaaa
     2821 gaaggtgctg acgagcttgt ctttcttgat atcactgcat cagaacaggg tagggaaaca
     2881 atcaaagcgc ttgtttccaa agtggctcaa gtaattgaca taccatttac tgtaggtggt
     2941 ggagtaaaaa cactacaaca tgctagggat atcttgctaa gcggagctga caaagtggca
     3001 ataaacactg gcgctgtaaa aaatccagaa atcttgacaa agctcatgaa aatttttgga
     3061 aaacaatgtg tcgttgtggc catggacgta aagcgtaact atgattttga aaagggaaag
     3121 cacgtctttt ctgcagattc taaaaaattc tggtttgaag tctacatata tggtggaaaa
     3181 actgccacgg gactagatgc aatagaatgg gcaaaggaag ttgaaaggct tggggctgga
     3241 gaaatcctgc ttaccagcat agacatggat gggacaaagg agggctatga tatccaacta
     3301 acaaaggaaa ttgttaacgc tgtaaacatt ccagtcattg cttcaggtgg atgtggaaag
     3361 ccagtacaca tgcttgacgt ctttttgcaa tctaatgtgg atgcggcctt ggcagcttca
     3421 atatttcatt acaagactca ttctgtagac agggtcaaag aatacctaaa agaaaacggt
     3481 gttcctgtca gaatatgaag aaagattaag aaaggaaaca caagtgtaat tcttatgcaa
     3541 aaaacaatag acgatattga tttttcaaaa ggtgatggac tggttcctgt aattgtgcag
     3601 gatatcgaaa caaaagaagt gcttactctt gcctatgcaa acaaagaatc aattgctctc
     3661 acacaaaaga caggtaactc ttggttttgg agcagatcaa gaaacaaact atggatgaaa
     3721 ggcgaggaat ctggcaatac tcaaaaagta aa
//
LOCUS       DATIDL010000099         2756 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrosopumilaceae archaeon isolate SMAG_U16342
            k141_1335739, whole genome shotgun sequence.
ACCESSION   DATIDL010000099 DATIDL010000000
VERSION     DATIDL010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935836
            Sequence Read Archive: SRR25158326
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosopumilaceae archaeon (soil metagenome)
  ORGANISM  Nitrosopumilaceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrosopumilales;
            Nitrosopumilaceae.
REFERENCE   1  (bases 1 to 2756)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2756)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/04/2024 05:09:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,520
            CDSs (total)                 :: 1,474
            Genes (coding)               :: 1,474
            CDSs (with protein)          :: 1,474
            Genes (RNA)                  :: 46
            rRNAs                        :: 1 (5S)
            complete rRNAs               :: 1 (5S)
            tRNAs                        :: 43
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2756
                     /organism="Nitrosopumilaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1335739"
                     /isolate="SMAG_U16342"
                     /isolation_source="soil"
                     /db_xref="taxon:2202732"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..835
                     /gene="tfb"
                     /locus_tag="VLB45_04345"
     CDS             <1..835
                     /gene="tfb"
                     /locus_tag="VLB45_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215007.1"
                     /note="stabilizes TBP binding to an archaeal box-A
                     promoter; responsible for recruiting RNA polymerase II to
                     the pre-initiation complex; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcription initiation factor IIB"
                     /protein_id="HSD04965.1"
                     /translation="EMFCGGCGFVLTERVEESGPEWRSFSKEEHEDRSRTGTPTSLAM
                     HDMGLATIIGPADKDASGKPLSASMKSTIERLRTWDSRSQVHEPVDRNFRQAFSELDR
                     LKDKLALSDAVIEKTAYIYRKALDKGLVRGRSIPGLVAAALYAACRDTETPRTLTDVA
                     NGINIKRKDVARCYRLLLRELDLKMPVVDPIKCVARIASKAGLSEKTKRKALLILKDA
                     AEIEISAGKDPMGLAAAALYLSCVMNGENKTQKDVAQAAGVTEVTIRNRYKGLKESLK
                     L"
     gene            867..1298
                     /locus_tag="VLB45_04350"
     CDS             867..1298
                     /locus_tag="VLB45_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04966.1"
                     /translation="MRKITYFVVAAIIVTVAILVLLSEPKSEKSIVYHITLADPKLYN
                     DNGIFTDFFEIQKGTYQFSFVPNGDSPQALSIALKGATFSFAEDFQLEGTAHDTGIST
                     YYTWDYLGTKEILVPEDQQLQIVINPNGNLLGSVSVDLIKT"
     gene            1604..2344
                     /locus_tag="VLB45_04355"
     CDS             1604..2344
                     /locus_tag="VLB45_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HSD04967.1"
                     /translation="MKLAGKVAIVTGGSRGIGKATAKLFVQEGANVMITAKDPVRLEN
                     AAKEIGASFVAGDTRNQNDVKNAVKKTIEKFGRIDILVNNAGIFPQIKPLHKISDQEW
                     NEVIDVNLTGPFRFTREVIPFMEKNGGSIINISSDAGLKAFENFEADAYTASKGALVL
                     LTKAWAIEYSKSKIRVNCVCPGVVETDMTKPYLTSESDRQMVAAEYPIGRIGTVEDVA
                     KAILYFASDDSSWTTGAILALDGGAAAK"
     gene            2425..2595
                     /locus_tag="VLB45_04360"
     CDS             2425..2595
                     /locus_tag="VLB45_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04968.1"
                     /translation="MLSVIWFIITLPLPFIIGNPSVAQTQLFIILQITVMISIPFVIL
                     AVAWTLKPELTT"
BASE COUNT          908 a          479 c          569 g          800 t
ORIGIN      
        1 agaaatgttt tgtggaggat gcggttttgt tcttaccgaa agggttgaag aatcaggtcc
       61 tgaatggaga tcattttcca aagaagaaca tgaagaccga agtagaactg gaactcccac
      121 ttcacttgca atgcacgata tgggacttgc aactataatt ggtcctgcag acaaggatgc
      181 ttctggaaaa cctctatcag cttctatgaa aagcacaata gagcgtctca gaacttggga
      241 tagcagaagc caagtacatg agccagtaga cagaaacttt agacaggcat tcagtgagct
      301 tgatagacta aaagacaagc ttgcactttc tgatgcagta atagaaaaaa cggcatatat
      361 ctacagaaaa gcacttgaca aaggtcttgt cagaggtaga tcaattccag gacttgtagc
      421 tgctgcactt tatgctgcat gtagagacac agagactcca aggactttaa ctgacgttgc
      481 aaatggcatt aacatcaaaa gaaaagatgt tgcgcgatgc tataggttgc ttctcagaga
      541 acttgatctt aagatgcctg tggtagatcc gataaagtgt gtagcaagaa ttgcaagcaa
      601 agcaggactc agtgaaaaga caaagcgaaa agcattgtta attctaaagg atgctgcaga
      661 aattgaaatt tcagctggaa aagatcctat gggacttgca gcggctgcat tgtatctttc
      721 ttgtgttatg aatggtgaaa ataaaacaca aaaagatgtt gcacaggcag cgggtgtaac
      781 ggaagtcact atcagaaaca ggtacaaggg actcaaagaa tccctaaaac tataattttt
      841 tttataaata atccagtttt aactatatga gaaaaatcac atattttgta gttgcagcaa
      901 ttattgttac agtagcaata cttgttttat tatctgaacc caagtctgaa aaatctatag
      961 tttatcacat aacactagca gatcccaaac tgtataatga caatggaatt tttactgatt
     1021 tttttgaaat tcaaaaagga acatatcaat ttagttttgt accaaatgga gatagcccac
     1081 aagcactttc aattgcatta aaaggtgcaa ctttttcgtt tgcggaagat tttcagcttg
     1141 aaggaactgc acacgacact ggaatctcta cttactatac atgggattat cttggtacaa
     1201 aagaaattct agtaccagaa gatcaacaac tccaaattgt gataaatcct aatggcaatt
     1261 tgcttggcag cgtatcagtt gatttgatta aaacataatt ggcgtggccc cccgtcagaa
     1321 gggcagagat tacctctttg aatcggctcc aaggccggat ttcatttatc gttctgcggg
     1381 accacgcaat ttaagcagta gattttgcat ccttctgcat gttttattat aggacttgta
     1441 agtatttatg acttcatgtt gatttttcta tggtttggtc tacttttaag agattttttc
     1501 aagtaaaaaa tttagatcga ctaaatgata agctgattta ccttgatttt tgcaagaaaa
     1561 tgacattgta taaactcaaa aagtcttgga aatatcctat tttatgaaac ttgctggaaa
     1621 agttgcaata gtgactggtg gcagccgtgg aattggcaag gctactgcca agttgttcgt
     1681 acaagaaggt gctaatgtca tgatcactgc aaaagatcca gtacgattag aaaatgcagc
     1741 aaaagaaata ggtgcatctt ttgtagcagg cgacacaaga aatcagaatg atgtcaaaaa
     1801 tgctgtaaag aaaaccattg aaaaatttgg tagaatagac attttggtaa ataatgccgg
     1861 aatttttcct cagataaaac cattacacaa aatttcagat caggaatgga atgaggtaat
     1921 tgatgtaaac ctgacaggac cgtttcgttt tacaagagaa gtcattccgt ttatggaaaa
     1981 aaatggtggt tcaatcataa acatatcttc ggatgcagga ctcaaagcgt ttgaaaattt
     2041 tgaggcagat gcatatactg catcaaaagg tgcgctggtt ttactgacaa aagcgtgggc
     2101 tattgaatat tccaaatcca agattcgtgt aaactgtgta tgtcctggtg ttgttgaaac
     2161 agacatgaca aaaccatatc ttacatcaga gtcagacagg cagatggttg cagcagaata
     2221 tcccattggg agaataggaa ctgtggaaga tgttgcaaag gccattttgt attttgcgtc
     2281 agatgattca tcatggacga ctggtgcaat cttggcatta gatggcggag ctgcagcaaa
     2341 ataataaggg caatcaaatt aatacgtcat aatcataggt tccttgtgaa tccaaagaaa
     2401 aagaagcgct tagagatagt cattttgctc tcagttatct ggttcataat caccttaccg
     2461 ttgccattca ttataggaaa tccaagcgtg gctcagactc agttgtttat aattttgcaa
     2521 attaccgtca tgatttcaat tccatttgta atacttgcag tagcgtggac tctaaaacct
     2581 gagcttacta cctaagatag aaactcttct gcaccaagac cttcgggagt gattttgatc
     2641 cagcggtttc ttcctatttg ctcaatttca acaaacttcc aatccctcaa aagaggctgg
     2701 attatgtttt tatcaagtga tgcaaatctt gcttgctgaa agttttcctc tcttgc
//
LOCUS       DATIDL010000100        24239 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrosopumilaceae archaeon isolate SMAG_U16342
            k141_1739305, whole genome shotgun sequence.
ACCESSION   DATIDL010000100 DATIDL010000000
VERSION     DATIDL010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935836
            Sequence Read Archive: SRR25158326
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosopumilaceae archaeon (soil metagenome)
  ORGANISM  Nitrosopumilaceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrosopumilales;
            Nitrosopumilaceae.
REFERENCE   1  (bases 1 to 24239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/04/2024 05:09:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,520
            CDSs (total)                 :: 1,474
            Genes (coding)               :: 1,474
            CDSs (with protein)          :: 1,474
            Genes (RNA)                  :: 46
            rRNAs                        :: 1 (5S)
            complete rRNAs               :: 1 (5S)
            tRNAs                        :: 43
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24239
                     /organism="Nitrosopumilaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1739305"
                     /isolate="SMAG_U16342"
                     /isolation_source="soil"
                     /db_xref="taxon:2202732"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..835)
                     /locus_tag="VLB45_04365"
     CDS             complement(<1..835)
                     /locus_tag="VLB45_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010191831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HSD04969.1"
                     /translation="MKLKDSSLNQIIKKTIVISPETSLLDARAILLRHKISRLIVARN
                     NKPVGIITEKDLVRAIYYLAGKPLEAIRVSEFMSRDLVSITERGTIDDCAKSMLSHKI
                     SSIIVLKKDGTLAGIITKTDLASIFLTQSSGPLPVAKVMTKKVVTVKPGDSLLYVESV
                     LINNKISRVVVERKRMPVGIITHRDFIPAKMPLWIKQYADPKEVEEFTLGINPHEYKS
                     NQLSYLLTFIATDIMTPNPVVIDAKEDVSTAALLMIRHGISGLPVVRNSLLVGILTKT
                     DI"
     gene            974..1519
                     /locus_tag="VLB45_04370"
     CDS             974..1519
                     /locus_tag="VLB45_04370"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HSD04970.1"
                     /translation="MTLVLLNEPVTKFMNTKMTTVASNLTIKDATKAMVDSKIDSILI
                     FEHGQIIGMVTEKDILHDVVAKGLDPTKTIIKKITKGPLITIQKNATVRDALKLMKKH
                     DIRRLVVMDKRPIGLITQKLVCGNLEDTRVLLPELEIPDKIRCPYCLSQFENKKLLSK
                     HIDDIHIGRGLLEGNLKKTFS"
     gene            1553..2140
                     /locus_tag="VLB45_04375"
     CDS             1553..2140
                     /locus_tag="VLB45_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04971.1"
                     /translation="MEPTKVKRQPHPLEHEKTRSITYRLPARIVEELETESMQREISQ
                     NVLVKQILEKYLQWDRFASTIGMIPVPKDVLKTLGEEMKGDEINKIIDVMLPLLKDWI
                     MFMKGGYDLKRAIESLEDYMRSSGMISDHRIEGNIHHFIIQHNLGMKWSLFTEQLLKH
                     VFHQFLPETALKCRTTPNTTIASIALGSDFSEHSY"
     gene            2231..3076
                     /locus_tag="VLB45_04380"
     CDS             2231..3076
                     /locus_tag="VLB45_04380"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HSD04972.1"
                     /translation="MISEYKKNRPVASYSLEELLPHSLFSSPAVSIRTIDTVSDAASL
                     LAHNLEYFTSTLVVTKDELPVGMIGGADLLDAIIKNPKFSDKTPINEIINNNLITVTK
                     QTRLDELLKTWLQTKRAFAIIPNQYHGYSAISARKLLEIGALCKTNMKISNIPKKKII
                     TFSKDHTIREVIIAMFENKTRKLILENTLSFISDRIITEKIIKEPKHLQNINTFLDMN
                     VNVCKLENVKTVSNDLEIPEACKIMHDMLHPYLMTGDQVVSPWDIVLILGSKDLIDYQ
                     WQNQK"
     gene            3134..3565
                     /locus_tag="VLB45_04385"
     CDS             3134..3565
                     /locus_tag="VLB45_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HSD04973.1"
                     /translation="MSHTFVKDIMKKTVISVDSSMTVKDAAKMMKDAGVGCVVVMEKN
                     LAVGIVTERDFVRKVIAQEKPSSIVIKEIMSSPLIVINPDETVWELAELMKQRRIHKV
                     PVTEGERLVGIATSTDLTKLCSIGSDSEMRKICDQILLRMK"
     gene            complement(3598..3939)
                     /locus_tag="VLB45_04390"
     CDS             complement(3598..3939)
                     /locus_tag="VLB45_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007551714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HSD04974.1"
                     /translation="MVTSLIRIEAVIPQNDVKAVSVALKKLSVGGITVSKVRGRGKTI
                     AAELHASKGTEIFIPEFSDKYILQVIVSDNREHDVIDIIRQNSKIGKIFISDIKRAID
                     IATGAEDEKAI"
     gene            4043..5302
                     /locus_tag="VLB45_04395"
     CDS             4043..5302
                     /locus_tag="VLB45_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HSD04975.1"
                     /translation="MPIDTGDTAWMLIASSLVLLMIPALGLFEAGLLRKKNTVSVFMQ
                     IFFGMALLSVMWFTFGFSLTFGPDTGGLVGNLDWTFLKGVPFDDSLPMAPTIPGVLFA
                     KFQMMFAVITPLLLTGAIAERMKFSAYILFIAAWSALIYYPLVHWMWGGGWLGAMGVV
                     DFAGGIVIHTSAGMGALAAALVLGRRRNYGPSIMIPHSIPIAVLGSSLLWLGWFGFNA
                     GSALASGGVAGNTVIVTHMASAISALIWVGLSWLRTGKPSVIAAINGAIAGLAGITPA
                     SGYVSVEHSFVIGIAIGLASYLGVLFFKERLKIDDALDVSSVHGVAGIIGSIAIGLFA
                     SSMINPSGPNGLLFGNPEQLLLQAMGVGVAGALGFGGTYVIMKVIDVLIGVRVTPKTE
                     DAGLDIEEHAERAYADEDEFGPAEQPK"
     gene            complement(5314..7065)
                     /locus_tag="VLB45_04400"
     CDS             complement(5314..7065)
                     /locus_tag="VLB45_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014966040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HSD04976.1"
                     /translation="MQIQFFSLDKKLIFLVMIVSIIALSTTAVLSFNFSQDVLKDRIN
                     DQLISESDLRGNAIRNVFDTRIKETQILSTDPMIQNLVEELNQNQHDSDLKSLVKEKR
                     GAFLTEIKTFRELVGYSIGFFDVKIIGNQGVVYFSLGKLSDSDFSQDPRYVLGLTGPF
                     VDIEPTSNEGRKIVVSTPIFSRENGSNSEPIGVIIADMSTTAIDDILLNRGGLGNTGE
                     VYLVNDDFFMVSESRFIENAAFNQKVNTLPVQDCFERNVETGGLYADYRNVMIYGYSY
                     CAKDLGLVLLAEVDESETFRPVLILQDKIFQAGILITIGMGAGTYFLSKLISRPIIKL
                     KNAANEIANGNFDVRTNIKSGDEIGALSTSFDSMAKKIQESLIAIKQREDVIKHQQDI
                     LLHFSDYSANYFVCIIDIISSTKITARLTDLESSKLYTTFLNSMATIVDQFEGVVVKN
                     IGDALLFYFPKTNAEEKEHLKNALECCFKMSESHKEINTKLENQSLPSINYRISATYG
                     SVRIAKVATSSIEDIFGSTVNRCSKINPLAPPNGIAIGDDLYQLVKNFDEYVFRKIDD
                     YELTYEQDYSVYSVSRK"
     gene            7179..7655
                     /locus_tag="VLB45_04405"
     CDS             7179..7655
                     /locus_tag="VLB45_04405"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HSD04977.1"
                     /translation="MVRITPEVQEMLRKQKFIVVGSVDLNGLANLSPRTSFTFGEDTI
                     CWLDFFKHKSHINFKSVPWVSVAVFDAKKLKGFQLKGKVTFVTDPKQKSEIVDKITRS
                     VTGATSSKVFERMNEGKPPDVVMFKTKAIYSLSPEETSGVALVLDTDGETVSLLGI"
     gene            complement(7641..9050)
                     /gene="gatB"
                     /locus_tag="VLB45_04410"
     CDS             complement(7641..9050)
                     /gene="gatB"
                     /locus_tag="VLB45_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HSD04978.1"
                     /translation="MTKIGLEIHCQLTKLESKLFCSCKADYREFETNSNLCPVCVGLP
                     GSLPVLNKNAVEKAAMIAMALGCKTPHKISFFRKNYFYPDLPKNFQITQYNAYGPTSI
                     GHDGKIMIEEKEIRIRRIQLEEDPGRLVYGGSSEKTKTTLVDYNRAGTPLVEIVTEPD
                     FETPKQVRIFLNILADLVENLGVSDPSLDGALRVDGNISIEKGNRVEIKNVGSFRDIE
                     KALHFEITRQQSLAERNIPIPAETRHWDEARKITISARSKEEEQDYRYFPESDIPTIM
                     IDDIVFEKLRQNMPESVLSKKDRYVNKYGIPLQVAEVLSSDKFYSDLFEQAHNDNNAK
                     EIANIITTDLKGFVDTNEQREQLKLTPKHLSDLADAIMNNKITRNSGKTALQEIVKTG
                     KSLSEIISSLDLGHVDDESSLSKTIDEVFVEENNAVSEAKTNPDTINYLVGKVMRKTK
                     GKANPTLTLDIIRKKLDSQ"
     gene            complement(9047..10492)
                     /gene="gatA"
                     /locus_tag="VLB45_04415"
     CDS             complement(9047..10492)
                     /gene="gatA"
                     /locus_tag="VLB45_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HSD04979.1"
                     /translation="MSLSISATQYVKQVQSGATSAEEFVAKTLERIQKVEKTINAFIT
                     INDNALNQAKEIDKKLKSKEKTGICLGMPVSIKDNICTKGIKTTCASKMLENFVAPYD
                     ATVISRLKSEDAIIIGKTNLDEFAMGSTTEFSYYGPSKNPWNPDYVPGGSSGGSGVSV
                     SALECVASLGSDTGGSVRSPASFCSVVGLKPTYGLISRYGLVSYSNSIEQIGPLARTV
                     EDIAFMLNIISGHDSHDNTTIDSGKPDYTKDLDAGVSGKKIGIISEMIGEGVDKDVLA
                     ATNRAISKLERLGAECSPISLDSAKHSVAAYYTIASAEASSNLSRYDNIRYGFDFDHE
                     GYEYNAYISKARSHFGPEVTRRMLLGTFVLSSGYYGKYYLKAQKVRNLIKSQLDAAFK
                     KVDLLVAPTMPILPFKIGEKIDDPLTLYLLDINTILANLSGIPAISVPYEIAHGLPIG
                     VQFFANSLQEKPLLQAAYALHKTTNLPEVPI"
     gene            complement(10495..10758)
                     /locus_tag="VLB45_04420"
     CDS             complement(10495..10758)
                     /locus_tag="VLB45_04420"
                     /inference="COORDINATES: protein motif:HMM:NF014716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04980.1"
                     /translation="MVKKEEIEQLGHLIKIEIKDPQKYIKQVEQIINYFDTLDKVTID
                     SDKILTQELTLDKLREDKYIPYEKNLVEKLKHTSEKFVKAPKM"
     gene            complement(10767..12080)
                     /gene="aspS"
                     /locus_tag="VLB45_04425"
     CDS             complement(10767..12080)
                     /gene="aspS"
                     /locus_tag="VLB45_04425"
                     /EC_number="6.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA(Asn) ligase"
                     /protein_id="HSD04981.1"
                     /translation="MIDTELGTWRRTIYSNQINPSMDGKEVIVMGWVASVRDHGNLVF
                     MMLNDKEGEIQITAKAGSCSDEIKQAIVKLKEQSSIAIKGTIKPSPKAPHGAEIVPLE
                     LRIFSQVEKIAPFTSQTKIVPNIDTRLEIRAVDLRRSVLQHVFKARSLVLKSIRDYLY
                     EKEFVEVNTPKMIATATEGGAALFPIFYYNKEAFLAQSPQLYKEQLTMSFEKVFEIAP
                     IFRAEPSRTNRHLSEAISIDLEEAFSDYNDVMKHIENIIKRSIKAVKEFHETSNTGIE
                     LSIPDIPNSIPQYTYTELVEKMQKAGAKTEWGDDLYPSNLKKINLTGFYFIKNWPLGP
                     KPFYVKANKSDQKISESFDLMFGDLELSSGSTRIEKREELEERMKNKGFKLDAFDYHL
                     KVFDYGVPPHAGCGIGLERLMMALTGTENIRDVTFYPRDVDRLTP"
     gene            12177..12374
                     /locus_tag="VLB45_04430"
     CDS             12177..12374
                     /locus_tag="VLB45_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007401664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04982.1"
                     /translation="MPLTAREKAIVSITNAIMIYSQKMEDGTISPNQSIIDFVLKLVP
                     SDLKSEISIELIDEIFSHVSN"
     gene            12426..13451
                     /locus_tag="VLB45_04435"
     CDS             12426..13451
                     /locus_tag="VLB45_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--phosphoribosylaminoimidazolecarboxamide
                     ligase"
                     /protein_id="HSD04983.1"
                     /translation="MTSIATLGSHCALQVLKGAKDEGFKTILVCEKKREKLYRRFRFI
                     DELIMVDSFSEITEQRCTSKLKDQKSIIIPHGTLISQMDSSQIESIKTPIFGNKWTLR
                     WESDRKMKEKLMLESKLNIPKSVSSPKDIKNLVIAKRHGAAGGKGYFLTTSEKDYIKK
                     RDKLISQGLITGDNDLYLQEYSFGVSAYLQYFYSPLKEELEFFGVDRRYESDIDGLGR
                     IPAQQQIEVDSIPSFNVIANSPLVLRESLLDEVYSMGERFVEATKRLVKPGMNGPFCL
                     EGVYDENGKFTTFEFSARIVAGTNLYVDGSPYSTFLYDEHMSMGRRIAREIKEAEKTN
                     SITKIVT"
     gene            complement(13454..14563)
                     /locus_tag="VLB45_04440"
     CDS             complement(13454..14563)
                     /locus_tag="VLB45_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007551026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEFG-CTERM sorting domain-containing protein"
                     /protein_id="HSD04984.1"
                     /translation="MNRNFFGFVLVLLFSISVIPAFSEEDDEKLSFGSYLEETLGHLW
                     ALEQNLDDNNKELAIIHATHPVAELYDLMKPELEEHDAALDAQLRQILFELNEKTTSG
                     VTREQAQEAIDQAKDVVEIARATVIGSEGEKMTFKINLMINLLETSIVEYEEAVSDGQ
                     IREMAEFQDGSAFVWRSQQIYETIKSEVPEHEAEEIAEFYEDLWKAYDERTDPETVET
                     LASGIVHELEEVAGIEMEVTELGDYVTNIRSLLTEAKEEYEKGETDEALSFATKAYLD
                     NYEFLESAVGAQDPELNEEIEHMMREELRDMIKNGAPVSEVSAHIDEILLKMDTVAVI
                     VPEFGPMAFVVLVTAIVTIVALSIKYQKLNLVKRF"
     gene            14765..15397
                     /locus_tag="VLB45_04445"
     CDS             14765..15397
                     /locus_tag="VLB45_04445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04985.1"
                     /translation="MKQPTNNLRIATLTAIAAILLIPSMQGTQVFAEEKAANSNSVND
                     ITLKAVFDFRSGVKEKVDTFKNYKPFVQTGTSTTSGFDTTRSAPKLVLEKVMGADTPY
                     LYQAVDITHNRGKSVQHEYQEFDMAISWIQGNTPIRQIFYTDCNVADYFIYTEFDKEE
                     TYNEKTKFVYLDSFTFECRGLEYGNPVYHQILDDKVTQEKAVLKAAHNKK"
     gene            complement(15434..17554)
                     /locus_tag="VLB45_04450"
     CDS             complement(15434..17554)
                     /locus_tag="VLB45_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HSD04986.1"
                     /translation="MKRSRRVRIGIDVGGTFTKAVGIDIVTGQILGKSTVPTTHTSDT
                     GVSTGIVTALENLLSSSNIQLDEVELIAHSTTQAINALLESDTAKVGIIGMGVGLEKQ
                     NVIKRTNLQDPSINSRQDLKTAYRFLDTSHLITESEIDNAIQELKNEGAEVIVASEAF
                     SVDDPSNELYVMNVAMAKNMPATASHEISGIYGLEIRTLTAAINASVLPKTFEVANFV
                     EKAIRGTGVSAPLMIMKGDGGVTDMNTFRTKPILTILSGPAASVAGALLYLKVTNGIF
                     VEVGGTSTNICIIRNGKPEIRYVTIKDHPTCIRSMDVRVVGVAGGSMVRLKDGRVWKI
                     GPRSAHIAGLKYSCYADPEDLKTGEIVMIKPRPNDQDEYVAIKCKGGTYAITNTCAAN
                     ALGMIDEGDYAFVNKTSAKIAIGMLAQKIGISVSEMALSIIQTASFEITKTISQILKE
                     FKMDKERTTIIGGGGGTSVLVPFVAKQLGISYKKAEHAEIISSIGVASSMIQEELEHT
                     IDNPTAEQISHIHKKIHALVVEKGAVPESVVINSEYIPEKSVLRVTAIGNVEYEKETA
                     KNVFTLEDALVRANEIMQIPQDLIELSFETDNYFVFTGHIEKKKLFGKKKHHHILILD
                     RYGRMKLSVKNGKLFQGGTLSVIEEFEEFLESRHGEIAPQVHLLNDLKLIDFSGLTSA
                     ANIVIAIKKELEGSTKGAIVLELQ"
     gene            complement(17541..19775)
                     /locus_tag="VLB45_04455"
     CDS             complement(17541..19775)
                     /locus_tag="VLB45_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007550584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HSD04987.1"
                     /translation="MARFGVLYYIIVFAIIGSFVLPMQNISAQSQEQIIFLSDKELTF
                     VGVDLAEQSIMKENYDAASVYLKFASDAFARSLPSARSVDNNLADEIHIMLVEVQADT
                     TSRASNDVILQKISNIKSLLEKIQLDSSLTPNVLAQMLLVADETYENGSLKNDEVSYQ
                     LSYSIISKVEQIFESSTSFDERQRTELQSFFSELDSLVSSRENFVKIGTLITAIQRDL
                     LGTQSTVNQDNLYDVIRQLYKQLVIEIDAGNYARAEQIAIEAYLENFEYLEPDIEVVD
                     SELLHKLEIAMREDLRITIKQHTSSDIIKQKIDVILLDLDKAEDLVSKLPKANLAPTE
                     LTIASINDRDLAPMGSATAEQKSGVQTQIDFIRESLQSLLVQYKSGDYQSAYATARTA
                     YLDSYEHVEIPLRAIDPNFTLEAEIQFAELRNLINTRADYEKVQEATIAIKRSLDESE
                     RLVSGTGALAPAIAFSSSFALIFREGLESVLILGAILTYLEASRNTKFKPYVYYGVVA
                     AIAATAITWFIASYVIEISGANRELIEAVAALSATAVLFYVSFWILNKIETKKWMEFV
                     KAKVWQATATGGFAVFVMLSFFTVYREGFETVLFYQAMFGFAKYMEAYVGLGFVLGIA
                     VLFGVYYGMRRLGRRLPLKALFGLTMGVGAYLSIAFIGNAIRELQTLDIIPVTNMIGT
                     IPRLDINLSTMTGIHPTLETTIAQIVLLGIYLAAAFYVLIMRPKREEQIAKMRKSRAQ
                     VEEI"
     gene            19888..20217
                     /locus_tag="VLB45_04460"
     CDS             19888..20217
                     /locus_tag="VLB45_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007550586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HSD04988.1"
                     /translation="MVKRLSLTQLKKYDVNQKVLEALADAQSRAILFSVIKEGKTAAE
                     LAEEHRIPLSSVYKKISDLEELSLIKIEKWILSDNGKKFKLYKSRISKADVSIRKPEP
                     VISLSPN"
     gene            complement(20341..21075)
                     /locus_tag="VLB45_04465"
     CDS             complement(20341..21075)
                     /locus_tag="VLB45_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007401672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbtA family protein"
                     /protein_id="HSD04989.1"
                     /translation="MKTFLFLIIVLGSGFSAGLVHGIVNLGVVEPFLDDAIGIENQNL
                     FASGEAENTPEFWEEFNMYRTWQKGGEVLAGGILGLSLGALFGLVFAYSRHILPGKHH
                     VKKALILAGIMWFTLFFVPFLKYPANPPTVGDPETIMIRTLLYISFVALSGLGALAFS
                     KIYKKMQNQAKKFVVPLGYAIYISIIFVLMPPSPDEVIAPVDLLNGFRIASASTVTMF
                     WVINAIILGSLWQKFQPHVEQNQESR"
     gene            complement(21076..21297)
                     /locus_tag="VLB45_04470"
     CDS             complement(21076..21297)
                     /locus_tag="VLB45_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbtB domain-containing protein"
                     /protein_id="HSD04990.1"
                     /translation="MKLTNTLQITKSNHGVPKLAVGILLALFGIGVFVVGFDQGHLFS
                     MIEGSSAFDDLYLHELYHDMRHAAGYPCH"
     gene            21394..22035
                     /locus_tag="VLB45_04475"
     CDS             21394..22035
                     /locus_tag="VLB45_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HSD04991.1"
                     /translation="MKRYYLLAIPAVIAIIFSYYYLVPTEPEEDTIITTQILMQNGSP
                     LIGNPDAPITIVEWGDYQCTFCYKFHQSSKDLLIQEYVDAGKVNFVFRDFPLNGPDSI
                     LAAEASYCADDQGKYWEYHDELYKNWAGEKTGWVNRESLDRFANTVNLNLEQFNKCLD
                     EKKYEQKVSDNYEFGASIGIGATPSFLVFNNEKVTKIVGNQPFSVFRQVIDTL"
     gene            22072..23346
                     /gene="prf1"
                     /locus_tag="VLB45_04480"
     CDS             22072..23346
                     /gene="prf1"
                     /locus_tag="VLB45_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor aRF-1"
                     /protein_id="HSD04992.1"
                     /translation="MGKPIIEKQDSVKLYKLRKTLEDLSDKSGRGTELISLYIPPKKA
                     LHEVINGLREEQGTADNIKSDLTRTHVVDALSRVIQRLKLYKRPPDKGLVFFCGALPP
                     EGGGPIGSEVIKMYELEPPKELQTFLYRCDDHFHVDILKDMLKADNLIGFLAIDAKDA
                     GWGLLQGDKLEVLGETSSGVAGKHRQGGQSARRFERLREMELGEYYHRVAHTTREYFI
                     DIYPIKGLIISGPGPTKENFIKEEYLEYRLQNMIISTLDTSYAGSEGVREAFARAQEV
                     LSDYRMVEEKRLVEKLFHDIHASRGLATYGLNEIIDLLKRNVVDTVIITDDTNLRKMD
                     VTCRRCQNVQTEILERPKVIQRMQELLRLPCPTCKSMDSEATERDIVDYLSLLTTDTG
                     TKIEVISGVTEHGAMLSSIGKVGAFLRYNPNQ"
     gene            23404..23721
                     /locus_tag="VLB45_04485"
     CDS             23404..23721
                     /locus_tag="VLB45_04485"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HSD04993.1"
                     /translation="MEHVLPEEFDAKILNNNKKTLVLFYADWCPYCANFRPTFEEINS
                     NIDKKAALVNEDENPLWDRFNIQAVPTMIAFENGKIISRKDARKGIGLTRSDMESIVK
                     ELS"
     gene            complement(23713..24126)
                     /locus_tag="VLB45_04490"
     CDS             complement(23713..24126)
                     /locus_tag="VLB45_04490"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HSD04994.1"
                     /translation="MNCLFCDILSGKTSAHFVYEDKSHVAIMDKYPIHRGHTLVIPRE
                     HHEKIIDMPEQNVSSLFSKIPLIARGVLEATGADGFNIGQNNGRAANQIIPHVHVHVI
                     PRYTRIGVAWPKRTIADEKDLADLAAKIKSCIKLT"
BASE COUNT         7693 a         4500 c         4236 g         7810 t
ORIGIN      
        1 ctatgtcagt ttttgtcaat atacctacca ataatgaatt tcttacaaca ggcaatccac
       61 ttattccgtg gcgaatcatc agcaatgctg cagtagacac atcttctttt gcatcaatca
      121 caactggatt tggagtcatt atgtctgtgg caataaacgt aagcaggtag cttagctgat
      181 ttgatttgta ttcatgagga ttgattccca aagtaaattc ttcaacttct tttggatcag
      241 catattgttt aatccataat ggcatttttg caggtataaa atcacgatgt gttattattc
      301 caacaggcat tctttttcgt tccactacca cgcgtgaaat tttattattg ataagtacac
      361 tttccacata aagaagtgag tcaccaggtt ttactgtgac tacttttttt gtcattactt
      421 ttgctactgg caagggtcca gaagattgtg tcaaaaatat agatgcaaga tctgtttttg
      481 taatgatccc agccaaagta ccatcttttt ttaatacaat tatggaactt atcttatgag
      541 atagcatcga tttagcacaa tcatctattg tgcctctctc tgttattgac acaagatctc
      601 ttgacatgaa ttcgcttacg cgtattgctt caagtggctt gccagctagg tagtaaatgg
      661 cacgaacaag atctttttca gttattattc ccacaggttt gttatttcgt gcaacaatca
      721 atctactaat cttgtgtcgc aacaatattg ccctagcatc aagaagtgaa gtttccggtg
      781 aaataacgat tgttttttta attatctgat tcaaactaga atcctttagt ttcatttcaa
      841 tcatcatgtc attttttgaa taatatatgc cttctaaaaa tctcaacaat gagaatcaat
      901 aatgataatt ccgttagttg ccatcaagat caaaagacaa cctttaagtt aaattttgaa
      961 atagccatga tacatgacat tggtacttct taatgagcca gtcacaaaat tcatgaatac
     1021 aaaaatgact acagtagcta gcaatctaac aataaaagat gccactaaag ccatggttga
     1081 ttcaaaaatc gatagtattt tgatttttga acatggtcag ataattggaa tggtaacaga
     1141 gaaagacatc cttcatgatg ttgttgcaaa aggattagat cctacaaaaa caatcattaa
     1201 aaaaataacc aaaggtccac ttattacaat acagaaaaat gcaacagtaa gagatgccct
     1261 gaagcttatg aaaaaacatg atattaggag actggtagta atggataaac ggccaatagg
     1321 attaatcaca cagaaattag tatgtggaaa tctcgaagat accagggttt tactgccaga
     1381 actagagatt cctgacaaaa tccgttgtcc atactgctta tcacaattcg aaaataaaaa
     1441 attgctatca aaacacattg acgacataca catcggtcgc ggattacttg aaggcaatct
     1501 taagaagaca tttagctaaa aacatataga ttgttaaaaa caacgatccc tcgtggaacc
     1561 taccaaagtc aagcgacaac ctcatcctct agaacatgaa aaaactagaa gtataacata
     1621 taggcttcct gccagaatcg tagaagagct tgaaacagaa tcaatgcaaa gagaaatttc
     1681 tcaaaacgta cttgtaaaac aaattttaga aaaatatctc cagtgggata gatttgccag
     1741 taccataggc atgatcccag ttccaaaaga tgttctcaag acattggggg aggaaatgaa
     1801 aggggatgaa ataaacaaaa tcatagatgt gatgttacca ctgctaaaag attggattat
     1861 gtttatgaaa ggcggttatg acctaaagcg tgcaattgaa tcattagaag attacatgag
     1921 atcatcagga atgatatcag atcacagaat agaaggaaac attcatcatt ttatcataca
     1981 acacaacttg ggtatgaagt ggtcactttt cacagaacaa ttactaaaac atgtttttca
     2041 tcaatttctt cctgaaactg cactaaaatg tcgtactaca ccaaatacca caatagctag
     2101 catagcttta ggttcagatt ttagcgaaca cagttattag tttcaattaa atggttttta
     2161 gattaaattc aaaatatcat atttgataat ctaaacatgg cttttaaaga aaaaacgtct
     2221 tgacagatca atgatctcag agtataaaaa aaatcgacct gtagcaagtt attcattaga
     2281 agaactttta ccacattcat tattttcctc tcctgcggta agcataagaa ctattgatac
     2341 agtcagcgat gccgcaagtt tgttggcaca taatctagag tattttacta gcacattggt
     2401 tgtcacaaaa gatgaacttc ctgtaggcat gataggcggc gcagatctac tagatgcaat
     2461 cataaaaaat cccaagtttt ctgataaaac accaataaat gaaattataa acaataacct
     2521 catcacagtt acaaaacaga ccagattaga tgaattgcta aaaacatggc tacagacaaa
     2581 gagggcattt gcaatcatac ctaatcaata tcacgggtat tctgcaattt ctgcacgaaa
     2641 attacttgag ataggcgctt tgtgcaaaac caacatgaag atttccaaca tccccaaaaa
     2701 gaaaatcatc acttttagta aagatcacac aataagagag gtaattatcg ccatgtttga
     2761 aaataagact cgcaagttga ttcttgaaaa tactttatca tttatcagtg atagaatcat
     2821 aacagaaaaa atcatcaagg aaccaaaaca tctacaaaat atcaacacct ttttggacat
     2881 gaatgtgaat gtgtgcaagc tagaaaacgt taagacagtt tcaaacgatt tggagatccc
     2941 agaagcatgt aaaatcatgc atgatatgct acatccttat ttgatgactg gtgatcaggt
     3001 tgtaagtcca tgggatattg tattaattct tggttcaaag gatttaattg attatcaatg
     3061 gcaaaatcaa aaatagttat catatgtgat aatcgcattt tgagttttta aatatgcaaa
     3121 tatgtggaat attatgagcc atacttttgt aaaagacata atgaaaaaga cagtaatctc
     3181 ggtagattct tctatgactg ttaaagatgc tgctaaaatg atgaaagatg caggcgtggg
     3241 ttgcgtagtg gtaatggaaa agaatctagc tgtgggaata gtgacagaaa gagatttcgt
     3301 taggaaagta atcgcgcaag aaaaaccatc atctattgta ataaaggaaa tcatgtcctc
     3361 acccttaatc gtaattaatc ctgacgaaac agtttgggaa ttggcagaat tgatgaaaca
     3421 gagacgcatt cacaaagtac cagtgacaga aggagaaaga ttggtaggaa ttgcaacttc
     3481 tacagatttg acaaaattat gcagtatagg atcagattct gaaatgcgca aaatctgtga
     3541 ccagattctt ttacggatga aataatctaa caaattggct taattgacaa aaatagatta
     3601 aattgctttc tcgtcttctg cgccagttgc tatatctata gcgcgtttta tatctgaaat
     3661 aaagatcttc ccaattttcg agttttgcct aataatatct ataacatcat gttccctatt
     3721 gtcagacact attacttgaa gaatgtattt atcgctaaac tcagggataa aaatctcagt
     3781 gccttttgaa gcatgcaatt ctgctgcaat agtctttcct ctacctctta cttttgatac
     3841 agttattcct ccaacagata atttttttaa tgcaacactt actgctttta cgtcattttg
     3901 tggtatgact gcctctattc taatcaagga tgtaaccatc tgatcaacta ttaaaaaaca
     3961 ttattcaaaa ttatcgatca tcattatcag atatgagaat cattccacat ttataataac
     4021 ggggttttcc ttaaaaaaac tcttgccaat tgatactggt gatacagcat ggatgcttat
     4081 agcatcaagc cttgtattgc taatgatccc tgcacttggg ttatttgagg caggattgtt
     4141 aagaaaaaag aataccgtat cagtttttat gcaaatcttt tttggaatgg cacttttgag
     4201 tgtcatgtgg tttacttttg gatttagttt aacatttggc ccagacactg gaggtcttgt
     4261 aggcaatctc gattggacat ttcttaaagg agttccattt gatgattccc tgcccatggc
     4321 accaacaatt cctggagtat tgtttgccaa attccaaatg atgtttgcag taatcacacc
     4381 gttgttactt accggcgcaa tagcagaacg catgaaattt agtgcatata ttttatttat
     4441 tgcagcatgg agcgcactga tctactatcc tcttgtacat tggatgtggg gtggtggatg
     4501 gcttggtgca atgggtgtag ttgattttgc aggcgggata gtaatccaca ctagtgcagg
     4561 aatgggagct cttgcagcag ctctagtttt aggcagaaga agaaattatg gaccttccat
     4621 aatgattccg cacagtattc caattgccgt tttaggatct tcattgttat ggttagggtg
     4681 gtttggattt aatgcaggca gcgcacttgc gtctggggga gtagctggaa atacagtaat
     4741 tgttacacac atggcatcag ccatatcagc tctcatttgg gttgggcttt cttggttgag
     4801 aacaggaaag ccaagtgtca tagctgctat caatggggcc atagcaggat tagcgggaat
     4861 aactcccgcg tcagggtatg taagtgtaga acattccttt gtaattggca ttgccatagg
     4921 tctagcatca tatcttgggg tgctcttctt taaagaacga ttaaagatag atgatgcact
     4981 tgatgtaagt tcggttcacg gggttgcagg aattattgga tcaatagcaa tcggactatt
     5041 tgcaagttcg atgataaatc catctggtcc aaatggatta ctgtttggca atccagaaca
     5101 actcttgctt caagctatgg gagtcggtgt agctggggca ttaggatttg gaggaacata
     5161 tgtaatcatg aaagtgatag atgttttgat aggagtcaga gtaacaccaa aaactgaaga
     5221 tgctggactt gatatagaag aacatgcgga gcgcgcatac gctgatgaag atgaatttgg
     5281 tccagcagaa caaccaaaat aaaaaataaa attttacttt cttgatacag aatatacaga
     5341 ataatcttgt tcatatgtga gttcataatc atctatcttt cggaacacat actcatcaaa
     5401 atttttcaca agttgataga gatcatctcc tattgcaatt ccatttgggg gtgcaagtgg
     5461 atttatcttt gaacatcgat taacggtaga tccaaagata tcttcaattg aggaggtagc
     5521 tacttttgca atcctaacag atccatatgt agcacttatt ctataattaa ttgatggtaa
     5581 tgactggttt tctaatttcg tattgatttc tttatgtgat tcagacattt taaaacagca
     5641 ctcaagagca tttttgagat gttctttttc ttcagcgttg gtttttggaa aataaaacaa
     5701 taatgcatca ccaatattct ttaccaccac accttcaaat tggtcaacta tagtcgccat
     5761 tgaattcaaa aatgtggtat acagtttact ggactccaaa tcagttagtc gtgcagtaat
     5821 ttttgtcgag ctgataatat caattataca cacaaagtaa tttgcactat aatcagaaaa
     5881 atgcaatagg atatcctgct ggtgttttat tacatcttct ctttgcttta tagctattaa
     5941 tgattcttgg atttttttag ccatggaatc aaacgatgtt gaaagtgcgc ctatctcgtc
     6001 acctgacttt atattggttc ttacgtcaaa attaccattg gcaatttcat ttgcggcgtt
     6061 ttttagtttt attattggtc ttgaaattaa ttttgataga aaatacgttc cagctcccat
     6121 tccaatagta atcaaaattc cggcttggaa tatcttatct tgaagaatca acacgggtcg
     6181 aaatgtttct gattcatcta cttctgccag caaaacaaga cctaaatctt ttgcacaata
     6241 tgaatatcca taaatcatca cgtttcggta atccgcatac agaccacctg tttccacatt
     6301 ccgctcaaaa caatcttgca ctggcaaagt gttaactttt tgattaaatg cagcattttc
     6361 tatgaatcgg gattcggaca ccataaaaaa atcatcattg actagataca cttcaccagt
     6421 atttcccaaa ccaccacgat ttagcaaaat gtcgtcaatt gctgtagtac tcatatcagc
     6481 aattattact ccgataggtt cagaattact gccgttttct ctactaaaaa taggcgtaga
     6541 tacaacaatt tttctccctt cattacttgt cggttcaatg tctacaaacg gtcctgttaa
     6601 acctagtaca taacgcggat cttgagaaaa atcagaatca gaaagtttac caagtgaaaa
     6661 gtacaccact ccttgattac caattatttt tacatcaaaa aagccaatcg agtaaccaac
     6721 caattctcta aatgttttaa tttcagttaa aaatgcacca cgtttctctt ttacaaggga
     6781 tttcagatca gaatcatgtt gattttggtt taactcttca accaaatttt gaatcattgg
     6841 atcggtggat aagatttgtg tttctttaat tctagtatca aaaacatttc gaattgcatt
     6901 tcctcgcaaa tcagattcac tgataagttg atcatttatt ctgtctttta gaacatcctg
     6961 tgaaaaatta aaactcaaaa ctgcggttgt actaagtgca attatagaaa caatcattac
     7021 caaaaaaatc agttttttat ctaaactaaa aaattggatt tgcatctaat caaactcttc
     7081 tttggttcaa tttataatat tcagatatca ttgtgtttct cattatcatc gtagataatc
     7141 atatttacct ttaattaaaa gactatagaa atacagacat ggttagaatt actcctgaag
     7201 ttcaagaaat gcttcggaag caaaagttca ttgtagtagg ctcagtagat cttaatggat
     7261 tagctaatct atcacctaga acatcattta cttttggtga ggatacgata tgttggcttg
     7321 attttttcaa gcataaatca catataaatt tcaaatcagt gccgtgggtc agtgtagcag
     7381 tttttgatgc aaaaaaattg aaaggatttc aattaaaagg gaaagttact tttgtcactg
     7441 atccaaaaca aaaatccgaa attgttgaca aaataactag atcagtgaca ggtgcaacat
     7501 cctcaaaagt ctttgaaaga atgaatgaag gaaaaccgcc agatgtggtg atgtttaaaa
     7561 caaaagcaat ctattctctc agtccagagg agacatcagg agtagctctg gtacttgata
     7621 cagatggcga aacagtctcc ttattgggaa tctaattttt tccgtattat atcaagtgta
     7681 agtgtaggat ttgcctttcc tttcgttttt ctcattacct tgcctacaag gtagttaatc
     7741 gtatcaggat tagttttagc ctctgataca gcattatttt cttctacaaa aacttcatca
     7801 attgtctttg aaagtgatga ttcatcatct acatgcccca agtcaaggct tgagataatc
     7861 tcagataacg actttcctgt ctttacaatt tcttgcaatg cggtttttcc agagtttcgt
     7921 gttattttgt tattcattat agcatcagcc aaatctgaaa ggtgctttgg agtaagttta
     7981 agctgctcac gttgttcatt agtgtctaca aatcctttaa gatcagtggt tatgatattt
     8041 gcaatttctt ttgcattatt gtcattatgt gcttgctcaa acaaatcaga ataaaacttg
     8101 tcagaagaca atacttcagc aacttgtaat ggaatcccat acttgttgac atacctgtct
     8161 ttttttgaca agacgctttc tggcatgttt tgtcttagct tttcaaacac gatgtcatct
     8221 atcatgattg ttggaatatc cgattctggg aaatatcgat aatcttgttc ttcttctttt
     8281 gacctggcag atatcgttat ttttcttgcc tcatcccaat gtctagtctc tgcaggaatg
     8341 ggaatatttc tttcagcaag gctttgctgt cgtgtaatct caaagtgtaa tgccttttca
     8401 atatccctaa acgagcctac attttttatc tcaactcgat ttcctttttc aattgaaatg
     8461 tttccatcaa ctcttaatgc gccatcaagg gaaggatctg agacccccaa attttcaaca
     8521 aggtctgcaa gtatgtttag gaatatcctt acctgttttg gagtctcaaa gtcaggttct
     8581 gtgactattt caaccagagg tgttcctgct ctgttgtaat caaccaaggt agttttggtt
     8641 ttttctgagc ttcctccata aactagcctt cctggatcct cttcaagttg tattcttctt
     8701 attcgaatct ccttttcttc aatcataatt tttccatcat gcccaatact agttggacca
     8761 tatgcattat actgggttat ttggaaattt tttggtaaat cagggtagaa ataatttttt
     8821 ctaaaaaagg aaatcttgtg tggtgtttta caaccaagtg ccatcgctat catagctgcc
     8881 ttttcaacag catttttgtt caaaactgga agagagccag gaagcccaac acatacaggg
     8941 caaagattag aattggtttc aaattctcga tagtctgctt tgcaagaaca aaataattta
     9001 ctttcaagct ttgttaattg acagtgtatt tctaatccta tctttgtcaa attggcacct
     9061 caggaaggtt tgttgtttta tgtaatgcat aagcagcttg tagcaatggt ttttcttgta
     9121 aagaatttgc aaaaaactgt acccctatag gtaatccatg tgcaatctca taaggcactg
     9181 aaatggccgg aattcctgaa aggtttgcca gtattgtatt gatgtcaagg aggtatagcg
     9241 taagagggtc gtcgattttt tctccgattt taaatggcaa tatcggcatt gttggtgcaa
     9301 caagaagatc cacttttttg aaggctgcat ctaattgcga ttttatcaga ttcctaactt
     9361 tttgagcttt taggtaatat tttccgtaat aacctgatga taatacaaag gttccaagca
     9421 gcatccttct tgtgacttca ggaccgaaat gacttcgtgc ttttgagatg tatgcgttat
     9481 actcatagcc ttcatgatca aaatcaaaac cataccttat attgtcgtat cgtgaaaggt
     9541 tactacttgc ttctgcggat gcaatagtat agtacgctgc aacagaatgt tttgcagaat
     9601 caagcgatat cggactacat tctgctccaa gtctttcaag cttcgatatt gccctgtttg
     9661 ttgcagcaag aacatctttg tcaactcctt ccccaatcat ttcagatatg atgccaattt
     9721 tttttccaga aactccggca tcaagatcct ttgtgtaatc gggttttccg ctgtctatgg
     9781 tagtgttgtc atgagaatca tgcccagaaa taatatttag cataaatgca atatcttcta
     9841 cagttcttgc aagcggtcct atctgttcaa tgctattaga atatgatact agaccatacc
     9901 tgctgatcag accatatgtt ggcttgagtc caactactga acaaaagctt gcagggcttc
     9961 taacagaacc accagtatca gagccaagag aggcaacaca ctctaaagca cttaccgaga
    10021 caccgcttcc acctgatgac ccgcctggaa catagtctgg attccaagga ttcttgctag
    10081 gtccataata actaaattca gtagtagaac ccatagcaaa ttcatccaaa tttgttttac
    10141 caatgatgat ggcgtcttca gatttcagtc tggaaatgac agttgcatca tatggcgcaa
    10201 caaaattttc aagcattttt gatgcgcatg tggttttgat tcctttggtg catatgttgt
    10261 ctttaattga gacaggcatc ccaagacaaa tgcctgtttt ttcttttgat tttaattttt
    10321 tatcaatttc ctttgcttga tttagcgcat tatcatttat tgtgataaaa gcgttaattg
    10381 ttttttctac tttttgtatt ctttctagtg tcttggcaac aaattcttca gcagatgttg
    10441 caccactttg tacctgtttg acatattgag ttgcagaaat tgaaagagac atacttacat
    10501 ctttggagcc ttgacaaatt tctcactagt gtgttttaat ttttcaacga gatttttttc
    10561 atatgggatg tacttgtctt ctcgtagttt gtctagagtc aattcctgag tcaaaatttt
    10621 atcagaatcg attgttactt tatcaagagt atcaaaataa tttatgattt gttctacttg
    10681 tttgatatat ttttgaggat cttttatctc gatttttatt agatggccga gttgttctat
    10741 ttcttctttt tttaccattt ttttccctac ggtgttagtc tatctacgtc tcttggataa
    10801 aatgttacat ccctaatgtt ttcagtgcct gtgagggcca tcatcagtct ttctagccca
    10861 attccgcatc ctgcatgcgg aggaacccca taatcaaaga ctttaaggtg ataatcaaag
    10921 gcgtctagct tgaatccctt gtttttcatg cgttcttcaa gttcttctct tttttcaatt
    10981 cttgtacttc cagatgaaag ctccaaatct ccaaacatga gatcaaatga ctcggatatt
    11041 ttttgatcag atttgtttgc ttttacgtaa aacggctttg gtcctagcgg ccaattcttt
    11101 atgaaataaa atcctgtcag gtttatcttt tttagatttg atggatatag atcatcaccc
    11161 cactctgttt tagctcctgc tttttgcatt ttttctacaa gctctgtgta agtatactgt
    11221 ggaatcgaat taggaatgtc aggtatgctc agttctattc cagtgtttga tgtttcatga
    11281 aattctttta ctgcttttat tgatctcttt atgatgtttt caatgtgttt catgacatca
    11341 ttgtaatctg aaaatgcttc ttccaagtct attgatattg cttcggatag atgtctgttg
    11401 gttctagaag gctctgctct aaaaattggt gcaatttcaa acactttttc aaagctcatc
    11461 gtaagctgct ctttgtaaag ctgtgggctt tgggcaagaa atgcttcctt gttgtaataa
    11521 aatataggaa acagtgcggc tccgccttct gttgctgttg caatcatttt aggagtattt
    11581 acttcaacaa actctttttc atagagataa tcacggattg attttagaac tagacttctt
    11641 gccttgaaaa cgtgttgtaa aacacttctt cgcaaatcaa ctgcacgtat ctcaagtcta
    11701 gtatctatat ttggaacaat ttttgtttgt gatgtaaacg gcgctatttt ttctacttgg
    11761 gagaatattc tcaattctag agggacaatt tctgctccat gtggtgcttt tggagaaggt
    11821 ttgattgttc cttttattgc aatgctggat tgttctttta attttactat ggcttgtttg
    11881 atttcatcag aacaagaacc tgcttttgca gtgatttgta tttcaccttc cttatcattg
    11941 agcatcataa acacaaggtt accatgatca cgaacacttg caacccatcc cattactatg
    12001 acttcttttc catccattga gggattgatt tgatttgagt atatggttct gcgccatgtg
    12061 ccaagttcag tatctatcat aagctaatct tttcatcaaa tcttctctaa ttagtgttaa
    12121 tttatcttcc tatcacaaaa ccattgtatt aattaggaaa aaattgtagc acgatcatgc
    12181 cacttactgc tagggaaaaa gcaatagttt caattacaaa tgccattatg atatattcgc
    12241 aaaaaatgga agatggcact atttctccaa accaatccat aattgatttt gttctaaaat
    12301 tggtacctag tgatttgaaa tcagaaatat cgattgaact aatagatgag atattctcac
    12361 atgtctcaaa ctaactcatg caactttcat aaagagccca attctatcta aaaacagggc
    12421 agacaatgac atccatagca accttaggtt cacattgtgc actacaagta ctaaaaggcg
    12481 caaaggatga gggatttaaa acaattctag tttgcgagaa aaaacgagaa aaactttacc
    12541 gcagatttag attcattgat gaactaatca tggttgattc attttcggaa ataacagaac
    12601 aaagatgcac atctaagcta aaagatcaaa aatcgataat aattccacat ggaacactga
    12661 tttcacagat ggattcttca caaatagagt ccatcaagac cccgattttt ggaaacaagt
    12721 ggacactacg atgggaatca gacagaaaaa tgaaagaaaa attaatgtta gaatcaaaac
    12781 ttaatattcc aaaatcagtt tccagcccaa aagatatcaa aaatcttgta atagcaaaac
    12841 gtcatggcgc tgcaggtggt aaaggatatt ttctaaccac aagtgaaaag gactacataa
    12901 aaaagcgcga caaattgata tcacaaggat taatcacagg cgacaacgat ctgtacttac
    12961 aagaatactc ctttggagta tctgcatatt tgcaatattt ctattctcca ctaaaagaag
    13021 aattagaatt ttttggggta gacagaagat acgaatcaga tattgacgga ttgggaagaa
    13081 ttccagctca acagcaaatt gaagttgatt caattccatc ctttaatgtg atagcaaata
    13141 gcccattggt gctaagagaa tcacttttag atgaagttta ttccatgggt gagagatttg
    13201 tcgaggctac aaaaagacta gtaaaaccag gaatgaacgg cccattttgt ttggaaggag
    13261 tatatgatga gaatggcaag tttactactt ttgagttctc tgcaagaatt gttgcaggaa
    13321 ccaatcttta tgtcgatggt tcaccgtatt cgacattcct ttatgatgag catatgagta
    13381 tgggaagaag aatagctaga gagattaaag aagctgaaaa aacaaacagt attacaaaga
    13441 ttgtaaccta gaactagaat cttttcacta ggtttagttt ttgatatttt atacttagag
    13501 caacaatggt aactattgca gtcactagaa ctacaaatgc cattgggcca aactcgggaa
    13561 caatcactgc cactgtatcc atttttagaa gaatttcatc aatatgcgca cttacctcag
    13621 aaacaggtgc accatttttg atcatatcac gtagttcttc gcgcatcatg tgttcaattt
    13681 cttcatttaa ttctggatct tgtgctccga ctgcagattc tagaaactca taattatcaa
    13741 gatatgcctt tgttgcaaag cttaatgcct catcggtttc tcctttttca tattcttctt
    13801 tggcttcagt caaaagagat cttatgtttg tgacataatc tccaagttca gttacctcca
    13861 tttctattcc agcgacttct tctagttcat gaacaattcc acttgcaaga gtttctacag
    13921 tttcaggatc tgttctttcg tcatatgctt tccacaaatc ctcataaaac tctgcaatct
    13981 cttctgcctc atgttctggc acttcagatt ttatagtttc atagatctgc tgtgatctcc
    14041 agacaaaagc agaaccatct tggaattctg ccatttcacg aatttgacca tcagatactg
    14101 cctcttcata ttctactatg gatgtctcaa gcagatttat catcaaatta atcttgaatg
    14161 tcattttttc accctcactt cctattaccg tagccctggc aatctcaaca acatcttttg
    14221 cctgatctat tgcctcttgt gcctgttctc ttgtcacacc agatgtggtt ttttcattta
    14281 attcaaacaa gatttgtcgt aattgcgcat caagtgcggc atcatgttct tcaagctctg
    14341 gtttcatcaa atcatatagc tctgccacag gatgtgttgc atgtataatt gctaactctt
    14401 tgttattgtc atcaaggttt tgttccaatg cccaaagatg ccccaaagtt tcttccagat
    14461 aactgccaaa ggataatttt tcatcatctt cttctgaaaa tgcaggaata acagatattg
    14521 aaaataacaa aactaaaacg aatccaaaga aatttctatt cattcaatac tgaacacctt
    14581 gatagattat ttatagtact tggatatttc aaattaatag aattgaactt tagaatatct
    14641 gcctcaacta aatattctac aattcaataa caaggtttta ttttaaacaa tggtttgcaa
    14701 aaaatgttcc agtgttttgc caagataaat tcaaataaat tatatcaaat ttgttttgat
    14761 tacaatgaag caaccaacaa acaatctaag aatcgctaca ttaacagcaa ttgcagcaat
    14821 tcttttgata ccaagtatgc aaggaacaca agtctttgca gaagaaaaag cagcaaatag
    14881 caacagtgta aatgatatca cactgaaagc agtgtttgat ttcaggtctg gagtgaagga
    14941 aaaagtagat actttcaaaa actacaaacc atttgtgcag acagggacat caactacgtc
    15001 aggctttgat actacacgaa gtgcaccaaa gcttgtatta gaaaaggtca tgggagcaga
    15061 taccccgtat ctttaccaag cagtggatat tacccataat cgtggaaaat cagtacagca
    15121 tgaataccaa gaatttgaca tggctatatc atggatacaa ggtaatactc caataagaca
    15181 gatcttctac acggactgta atgttgctga ctatttcatc tacacagagt ttgataaaga
    15241 ggagacatac aatgaaaaaa caaaatttgt ctacctagac tcgtttacct ttgagtgtag
    15301 aggactagaa tatggaaatc ctgtatatca tcaaattcta gatgacaagg taacacaaga
    15361 aaaagcagta ttaaaggcag cccataacaa gaaataattc ctcacttttt cttttttttt
    15421 gagtgcattc agtctattgt aattctaaca caattgcgcc ttttgttgaa ccttctaatt
    15481 ctttttttat tgcaatcaca atatttgcag ctgacgttaa acctgaaaag tcaattagtt
    15541 ttagatcatt tagaagatgt acttgagggg caatctcacc atgtcttgac tctaaaaact
    15601 cttcaaactc ttcaattaca gatagtgttc ctccctggaa tagttttccg ttttttactg
    15661 ataatttcat tcgaccatat ctgtctaaaa tcaaaatatg gtgatgtttt ttctttccaa
    15721 atagtttctt tttctcaata tgacctgtaa atacaaaata attatctgtc tcaaagctaa
    15781 gctcgatgag atcctgtggg atttgcatta tttcatttgc tcttaccagt gcatcttcta
    15841 gtgtaaagac attcttagca gtctcttttt catattcaac gttgccaata gcagtcacac
    15901 gtaaaaccga tttttcagga atgtactcac tgtttattac tacggattct ggaactgcgc
    15961 ctttttctac aacaagggca tgaatttttt tatgaatatg agaaatttgt tcagctgttg
    16021 gattatcaat agtgtgttct agctcttctt gaatcataga tgatgcaacc cctattgatg
    16081 aaattatttc cgcatgctct gcttttttat atgaaattcc aagttgcttt gcaacaaacg
    16141 gaaccaagac agacgttcca cctccacctc caataattgt tgttctttct ttatccatct
    16201 tgaattcctt taatatttgg ctaattgttt ttgtaatttc aaatgaagca gtttgaatta
    16261 tagaaagtgc catctcactt accgatattc ctattttttg ggctaacatt ccaattgcaa
    16321 tttttgcaga ggttttgttt acaaatgcat agtcaccctc atcaatcatt ccaagtgcat
    16381 ttgcagcaca agtgtttgta attgcgtatg ttccgccctt gcattttatt gcaacatatt
    16441 catcctggtc atttggacgc ggttttatca taacaatctc gcctgttttc aagtcttcag
    16501 gatcagcata gcatgaatat ttcaaaccag caatgtgagc acttcttggt ccaattttcc
    16561 aaactcttcc atcttttaat ctaaccatac tgcctccagc aactccaacg actctaacat
    16621 ccatagaacg tatgcaggta ggatgatctt ttattgtcac ataacgaatt tcaggtttgc
    16681 cgtttcgtat tatacatatg ttggtactag ttccgccaac ttcaacaaat atcccgtttg
    16741 tgacttttag atacagcaaa gcaccggcta cacttgcagc aggacctgat agaatagtaa
    16801 gaattggctt tgttctaaac gtgttcatgt ctgttactcc accatcacct ttcatgatca
    16861 tcagcggcgc agaaacccca gttccacgta ttgccttttc aacaaaattt gcaacttcaa
    16921 atgtttttgg cagtacgcta gcattgattg cagcagtcaa tgttctaatt tccaatccat
    16981 agatgccaga gatttcatgt gaggctgttg caggcatgtt ttttgccatt gcaacattca
    17041 tcacgtacag ctcattagaa gggtcatcta ctgaaaatgc ctcagacgct acaattacct
    17101 cagcaccttc attttttaat tcctgtatgg cgttatctat ttcagattca gtaataagat
    17161 gggaagtatc caagaagcga tatgccgttt ttagatcctg acgtgaattt atgctaggat
    17221 cctgcaaatt ggttcttttg attacgtttt gtttttctaa tccaacaccc attccaatta
    17281 tgccaacctt tgcagtgtca gattccaata atgcgttaat tgcctgagta gtactatgtg
    17341 cgattaattc aacttcatct aactgtatgt tagaagaaga tagcagattt tcaagtgcag
    17401 taacaattcc tgttgaaact cctgtatcac ttgtatgtgt tgtcggaaca gtagactttc
    17461 ccaaaatttg tcctgtaact atgtcaatac ctactgcttt tgtgaaagtg ccgccaacat
    17521 caatcccaat tcttactctt ctagatctct tcaacctgag ccctcgattt tctcattttt
    17581 gctatctgtt cttctctttt tggtcgcatt atcaaaacat agaatgctgc tgcaagatag
    17641 atcccaagta aaactatttg tgcaattgtg gtttcaagtg ttggatgaat tcctgtcatg
    17701 gtagacaagt ttatgtcaag tcttgggatg gttccaatca tattagtcac tgggattatg
    17761 tccagagttt gtaattccct tattgcattt ccaataaacg caatagaaag gtacgcgcca
    17821 actcccatgg ttagcccaaa taatgctttt agtgggagtc ttcttcctag ccttctcatg
    17881 ccataataaa caccaaacaa aaccgcgatt ccaagaacaa aacccaatcc aacatatgct
    17941 tccatgtatt tggcaaatcc aaacattgcc tgataaaaca agacagtttc aaagccctct
    18001 ctgtatactg taaaaaatga gagcattaca aagacagcaa aacctcccgt ggctgtggct
    18061 tgccagactt tggcctttac aaactccatc cattttttag tctcgatttt atttagaatc
    18121 caaaagctga cataaaacaa cactgcggtt gcagacagtg cagccactgc ttcaattaac
    18181 tcacgatttg caccagatat ttctataaca tatgaagcta taaaccaagt tattgcagtg
    18241 gctgcaattg cagctacaac gccatagtaa acatatggtt tgaattttgt gttacgcgat
    18301 gcctcaagat atgtcagtat tgcacctaga atcaataccg actctaatcc ttctctaaaa
    18361 atcagtgcaa atgaagatga aaatgcaatt gctggtgcaa gtgcacctgt acctgaaacc
    18421 aatctttctg attcatcaag acttcgtttt attgcaattg tagcttcttg aactttttca
    18481 taatctgctc ttgtattaat caaattacgt agttccgcaa actgaatttc agcttctaag
    18541 gtgaaatttg gatctattgc cctaagcgga atttcaacgt gttcatagct atcaagatat
    18601 gctgttcttg cagtagcata tgctgactga tagtctccag atttgtattg aaccaaaaga
    18661 gattgaagac tttcacggat aaaatcaatt tgagtttgaa ctccagattt ttgttctgcc
    18721 gtagctgaac ccataggagc aaggtctctg tcatttattg atgcaatagt tagttcagtt
    18781 ggagctaggt tagcttttgg aagtttggat acaagatctt ctgccttgtc aaggtctagt
    18841 aaaattacat caattttttg ttttataata tctgatgatg tgtgttgctt gatagttata
    18901 cgtagatctt ctctcatggc aatttctaat ttatgaagta gttcggaatc gactacttcg
    18961 atatcaggct ccagatactc aaaattttca aggtatgcct ctattgcaat ctgttctgcc
    19021 cttgcataat ttcccgcatc aatctcaata acaagttgtt tgtataactg gcgtatcaca
    19081 tcatacagat tatcttggtt aactgtactt tgggttccaa gcaaatcacg ctggatagca
    19141 gtaatcagtg ttccaatttt gacaaaattt tctctgcttg acactagaga gtcaagttct
    19201 gaaaaaaatg actgcaattc agttcgttgt cgttcatcaa aactagtaga tgattcaaaa
    19261 atttgttcaa cttttgagat tattgaatat gaaagttggt atgaaacttc atcatttttt
    19321 agagatccat tttcatatgt ttcgtctgca accaaaagca tttgtgctaa aacatttgga
    19381 gtcaatgaag aatctaattg gattttttcg agaagtgatt ttatgttaga aattttttgt
    19441 aaaatcacat catttgaggc acgtgaagtt gtatcagcct gaacttctac taacattatg
    19501 tgaatttcat cagcaagatt attgtcaact gatctagcag atggtaatga tctagcaaag
    19561 gcatcagatg caaattttag ataaacgctt gctgcatcat agttttcttt cattatgctt
    19621 tgttctgcca gatcaacgcc aacaaaagta agttctttgt cactcaaaaa aataatttgt
    19681 tcttgtgatt gtgcagatat attttgcata ggaagtacaa atgaaccaat tatagcaaat
    19741 acaataatgt aatacaacac accaaacctt gccacgaaat atcgttactt agggtaagct
    19801 aactttattt aaatcagaaa gtcattctaa aatcttagaa tgaggttcgc gtaatttaaa
    19861 tagttaggct gccctaattg aaaaaatatg gtaaaacgtc tctcacttac acaattaaaa
    19921 aaatatgacg taaatcaaaa agtgctggag gcgttagcag atgcacagtc aagagcaatt
    19981 ctattttcag tcataaaaga aggtaaaact gctgcagaat tggcagaaga gcacagaatt
    20041 ccactaagct cagtatacaa aaagatatca gatcttgagg agctttcttt aataaaaata
    20101 gaaaaatgga tactttcaga taatggtaaa aaattcaaac tttacaagag cagaataagt
    20161 aaagcggatg taagtataag aaaacccgaa ccagtaatca gtttgtctcc aaattagtcc
    20221 tttgatgggt agaccaagtg acagaagaca accaattatt ggtcggctga tgttgttgat
    20281 tttctagagt aatttatttc acaatcagtt tccatattgt tattacaaga ccttggaatc
    20341 ttatcgtgat tcctgatttt gttcaacatg tggctgaaat ttctgccaca agctaccaag
    20401 gattattgcg tttatgaccc aaaacattgt cacagtactt gcagatgcaa ttctgaaacc
    20461 atttagtaag tcaactggag caattacttc atctggacta ggaggcatca atacaaaaat
    20521 tatgcttatg tatatagcat aaccaagtgg cactacaaat ttcttagctt ggttttgcat
    20581 ttttttgtat atttttgaaa atgctaatgc accaagtcct gataatgcta caaatgaaat
    20641 gtaaagtaac gttctaatca taatagtttc aggatcgccc acagttggcg ggttagctgg
    20701 atatttgaga aaaggtacaa aaaataaggt aaaccacatt attcctgcaa gaattaacgc
    20761 ttttttgaca tgatgcttgc ctggcaaaat gtgccttgag taagcaaaga caagaccaaa
    20821 caacgcacct agtgacagcc ccaatattcc cccagcaaga acctcgcctc ctttttgcca
    20881 tgttctgtac atgttaaatt cttcccaaaa ttctggtgta ttttctgctt ctcctgatgc
    20941 aaacaagttt tgattttcta ttccaattgc atcatctaga aagggttcaa ccacacctaa
    21001 attaacaata ccatgcacta atcctgcaga aaagccagat cctaacacga taataaggaa
    21061 gagaaaggtc ttcatctagt gacacggata tcctgcagca tggcgcatgt catggtataa
    21121 ttcatgcaga tatagatcat caaacgcact tgaaccttca atcatactaa ataaatgacc
    21181 ttgatcaaat cccactacaa atactccaat tccaaacaat gcaagtaata ttccgacagc
    21241 taattttgga acaccatgat ttgattttgt gatttgtaaa gtatttgtca atttcatttt
    21301 caatgaaaat tatcagtatt taaattgtag gatggttcat ccgataattg aattttaaca
    21361 atcttaatat taaattcaaa attcaccgca tctatgaaga ggtattattt acttgcaata
    21421 cctgcagtca ttgcgataat tttctcatat tattatcttg taccaactga accagaagag
    21481 gacacaataa taaccactca aatactaatg caaaacggct ctcctctcat tgggaatcca
    21541 gatgcgccaa tcacgattgt agagtgggga gattaccagt gcacattttg ttacaaattt
    21601 catcaatcaa gcaaggattt actaattcaa gagtatgtgg atgcaggaaa ggtgaatttt
    21661 gtatttagag actttccttt gaacggtcca gattcaatat tggctgcgga ggcatcatac
    21721 tgtgccgatg atcaaggcaa atactgggaa taccatgatg aactctacaa aaattgggcc
    21781 ggtgaaaaaa caggatgggt gaatagagaa tcattggatc ggtttgccaa cacagtcaat
    21841 ctcaatttag agcaattcaa caaatgcctt gatgaaaaga aatacgagca gaaagtttct
    21901 gataattatg aatttggagc aagcattgga attggtgcaa caccgtcatt tttggttttc
    21961 aataatgaaa aagtaacaaa gatagtagga aatcaaccgt tttctgtttt tagacaagtc
    22021 attgacacac tataatcaaa cttaatattc gtgatctcaa gaggtcagat aatgggcaag
    22081 ccaataattg aaaaacaaga ctctgtcaag ctatacaaac taagaaaaac actcgaagat
    22141 ctttctgaca agtctggtag aggaacagaa ttgatatcac tttacattcc accaaagaaa
    22201 gcattacatg aagtcataaa cggattgcgc gaagagcaag gaactgcaga caacatcaaa
    22261 tcagatctca ccagaactca cgtagtagac gcactttcac gtgtaataca gagactaaag
    22321 ctatacaaaa ggcctccaga caaaggtctt gtttttttct gtggcgcact tccaccagaa
    22381 ggcggtgggc caattggcag cgaggtgatc aagatgtatg agcttgaacc accaaaagag
    22441 ttgcagacat tcctatacag atgcgatgat cattttcatg ttgacatcct aaaagacatg
    22501 ctaaaagctg ataatttaat tgggttttta gctattgacg caaaagatgc aggatggggt
    22561 cttttgcagg gagacaaact tgaagttttg ggcgagacat catcaggagt agcaggaaaa
    22621 cacaggcaag gaggtcagtc tgcaagaaga tttgaaagac tacgagaaat ggagctaggt
    22681 gaatattatc acagagtcgc acatacaact cgtgaatatt ttattgatat ttatccaata
    22741 aaaggactaa tcatatcagg tccaggaccg acaaaagaga atttcataaa agaagaatat
    22801 ctagaataca gattacaaaa tatgatcatt tcaacattag atacgtcata tgcaggctca
    22861 gaaggagttc gcgaggcatt tgcaagagca caagaagttt tatcagatta cagaatggta
    22921 gaagaaaaaa ggctagttga gaaactcttc catgacattc acgcatcacg aggtttggca
    22981 acatacggac taaacgagat aattgatctt ctaaaacgaa atgtcgttga tacggtcata
    23041 attactgacg acacaaatct aagaaaaatg gatgtaacat gcagaagatg tcaaaatgtt
    23101 caaaccgaaa ttttggaaag acccaaagtc attcaaagaa tgcaagaatt actaagatta
    23161 ccatgcccaa catgcaaaag catggattca gaagcgacag agcgagacat tgtggattat
    23221 ctttcattac ttacgactga cacaggaaca aaaatcgaag tcatatcagg agtaacagaa
    23281 catggcgcaa tgctttcaag cattggaaaa gtaggcgcct ttttgagata taatccaaat
    23341 cagtaaatga tgcaaatctg gtttttcgtt agctctaaat gttttacaat ctaacatcat
    23401 atcatggaac atgtcttacc agaggaattt gatgccaaaa ttctaaacaa caacaagaaa
    23461 acacttgtac ttttttatgc agactggtgc ccatactgtg caaactttag accaaccttt
    23521 gaagaaatca actcaaacat agacaaaaaa gctgctctag tcaacgaaga tgaaaaccca
    23581 ctttgggaca gattcaacat acaggcagtt ccaaccatga tagcatttga aaatggaaag
    23641 ataatttcta gaaaagatgc aagaaaagga attggactta ccagatcaga catggaatca
    23701 attgtcaagg aattaagtta attttataca gctttttatt tttgcagcca agtctgccaa
    23761 atctttttca tcagcaattg ttcgttttgg ccatgcaaca cctatgcggg tgtatcttgg
    23821 aataacatgg acgtgaacgt gcggtattat ttggtttgct gccctgccat tattttgtcc
    23881 aatgttaaat ccatctgcac ccgtggcttc cagtactcca cgtgcaatta aaggaatttt
    23941 tgaaaacaag cttgaaacat tttgttcagg catgtcaatg attttttcat gatgctcacg
    24001 tgggattaca agtgtgtgtc ctctatgtat tggatatttg tccattatag ctacatgcga
    24061 cttgtcttca tatacaaaat gtgccgatgt cttcccagac aagatgtcac agaagaggca
    24121 gttcatggat tgttgcgcat ttttatttta tttaaatcaa cggaaattta gtttctgatt
    24181 tattcacttc cagaaccgca tgaacaaaat ccatattcat ctattctagc tttacagag
//
LOCUS       DATIDL010000101         4082 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrosopumilaceae archaeon isolate SMAG_U16342
            k141_732323, whole genome shotgun sequence.
ACCESSION   DATIDL010000101 DATIDL010000000
VERSION     DATIDL010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935836
            Sequence Read Archive: SRR25158326
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosopumilaceae archaeon (soil metagenome)
  ORGANISM  Nitrosopumilaceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrosopumilales;
            Nitrosopumilaceae.
REFERENCE   1  (bases 1 to 4082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4082)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/04/2024 05:09:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,520
            CDSs (total)                 :: 1,474
            Genes (coding)               :: 1,474
            CDSs (with protein)          :: 1,474
            Genes (RNA)                  :: 46
            rRNAs                        :: 1 (5S)
            complete rRNAs               :: 1 (5S)
            tRNAs                        :: 43
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4082
                     /organism="Nitrosopumilaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_732323"
                     /isolate="SMAG_U16342"
                     /isolation_source="soil"
                     /db_xref="taxon:2202732"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            504..749
                     /locus_tag="VLB45_04495"
     CDS             504..749
                     /locus_tag="VLB45_04495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04995.1"
                     /translation="MDLLTNATVVDDAIRFVSESEKSKGKDREQIKRSSPSSSDISGN
                     EDDKESKEPDYDDENSDKEGEEKQEEQTGEITTNQVF"
     gene            complement(737..2296)
                     /locus_tag="VLB45_04500"
     CDS             complement(737..2296)
                     /locus_tag="VLB45_04500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HSD04996.1"
                     /translation="MIGFDGTIPFVGLFVLFIVLGFYGKYWRRGDLNHVHEWSLAGRR
                     LGTALVFFLIGADIYTAYTFVAIPSGVFAKGSLYFFAIPYVGLTFGVALVTMPRLWSL
                     SREKGYITASDFVKDRFSSKILSILIAMTGIISLLPYIALQIVGMQSVLTVMLSGTAN
                     SNTVEEISLLIAFVILAAFTYTSGLRGATLTAVFKDILIWITVIALIVVVPLSIGGGG
                     GGFATAFKNVKKQTYITLSESLVPGYTTLVLGSALALYLYPHAINGVLSSESAQKLRT
                     STALLPLYGVGLAIMALMGILVYAVPSAMNFLSHFPESSRGILVVPSLILYTMPGWFS
                     GIALLGIFVGGLVPAAIMAMSQASLLTRNIIKEIKPNMPASSEIRITKISSTAFKFVA
                     LGFVFIVPATYAISLQLLGGILIVQILPAVFFGLYVKSSLRKEPLIAGLLVGIFSGIY
                     MVEYTNNFGALTSSLVHTTFGSLYVAVIALVFNLIISFGGSAIMNKKRLLFSLLYYGK
                     KSKCDNNNQKT"
     gene            3074..3382
                     /locus_tag="VLB45_04505"
     CDS             3074..3382
                     /locus_tag="VLB45_04505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04997.1"
                     /translation="MSLFNNLKIAIDNPVTNDPTGYYSTDLVKEIMDEKESSPRLFVI
                     NLSEADIARTFCSEIINRVFRYRKNSFTLNPKIIFVFDEAQEFIPYEKKEKMEPKVLY
                     "
     gene            3619..3813
                     /locus_tag="VLB45_04510"
     CDS             3619..3813
                     /locus_tag="VLB45_04510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD04998.1"
                     /translation="MPRPYDRQLISDTFAIDDAFMDRALTFENGDWFMTSFKATNTQN
                     VPVFFDAKDNEDFLSEKIMK"
BASE COUNT         1434 a          707 c          742 g         1199 t
ORIGIN      
        1 cttgtccacc ataggattta attttagtta caacttcttc ggccttagtg gcgttgcctg
       61 catagttcac tgcaattgcg aagccatccc ctgccagcct tatagctact gcacgtccta
      121 tgccacgtga ggcaccggta acgatgacgc tcttcgttaa gagcattgta aagacattcg
      181 taagttattt gtgtaaacta aaactagtta cttggtttta ttatctatga acaacttcat
      241 tcagaagtaa ccaatccaaa gtaatcactt ttactttaca ttatatacta ccaaggtaat
      301 tacaatgctg ttaaggcatg tgtcatggat tattcacccg aggtacaaga agatagaaga
      361 tcgagtctag cctagcattt ccacaaacct aattagcaca agaacaaata atattgctac
      421 tagctatgaa ttacgtatca tggtagtaat agtacttttt cttattactt tggcagcagc
      481 tttgatgatg ataatggcaa tttatggact tgcttactaa tgcaacagta gtcgatgatg
      541 ctatcaggtt tgtatcagaa tctgaaaagt ccaaaggtaa agatagagag caaataaaaa
      601 gaagttcacc ctcttcttca gatatcagcg gcaatgaaga cgacaaagaa tctaaggagc
      661 ctgattatga tgatgaaaac tcggataaag aaggagaaga aaaacaggaa gagcaaactg
      721 gggaaataac aacaaatcaa gttttttgat tgttgttgtc acattttgat ttcttgccat
      781 agtatagtag ggagaacagt agtctttttt tgttcatgat tgcgcttccg ccaaatgata
      841 ttatcagatt aaacacaagg gcaatgactg caacatacaa tgaaccaaaa gttgtatgga
      901 ctagcgatga tgtaagtgct ccaaagttgt ttgtatactc gaccatataa attcctgaaa
      961 atattccaac aagtaaaccc gcaataagtg gttcttttct taatgatgat ttgacatata
     1021 gaccaaaaaa cactgcaggt agaatttgta caattagtat tcctcccaaa agttgtagag
     1081 atattgcata agtagctggc actataaaga caaaaccaag tgcaacaaac ttgaacgcag
     1141 ttgatgaaat ctttgttatt ctaatttcag aacttgcagg catgtttggt ttgatctctt
     1201 ttatgatatt tcttgtaagc aggcttgcct gtgacattgc cataattgct gcaggtacca
     1261 gaccacctac gaatatacca aggagagcta ttcctgaaaa ccatcccggc attgtatata
     1321 aaatcaaaga cggaacaaca agtataccgc gtgagctttc gggaaaatgt gaaaggaaat
     1381 tcattgcaga tggtacagcg tatacaagaa ttcccataag tgccataata gcaagcccaa
     1441 cgccataaag cggtagcaat gccgtactgg ttctaagctt ttgtgcgctc tctgagctta
     1501 aaacaccgtt tatggcatga ggataaagat agagcgcaag ggcgctgccc aaaaccagag
     1561 tcgtatagcc gggaacaagc gattcagaca atgtaatata ggtttgtttt tttacattct
     1621 tgaaggcagt agcaaaacca ccaccacctc caccaatgct tagaggaact acaactatca
     1681 gagcaatgac agttatccag atcagtatat ccttaaagac tgcagtgaga gttgctccac
     1741 gtagtccgct agtataggta aaggcagcca atattacaaa tgcaattaaa agcgatattt
     1801 cttcgactgt attagagtta gctgtacctg aaagcattac agtaagaact gattgcattc
     1861 ctactatctg taacgcaatg tatggcagta atgatatgat tcctgtcatt gcaattagaa
     1921 tagatagaat tttgctgcta aacctgtctt ttacaaaatc agatgcagtg atatatcctt
     1981 tttctctgga taacgaccaa agccttggca ttgtgacaag agcaacgcca aatgtcaacc
     2041 caacataagg gattgcaaaa aagtaaagcg agcctttggc aaaaactcct gatggaattg
     2101 caacaaaggt gtaggccgta taaatgtcag caccaattaa aaaaaagaca agtgcggtac
     2161 caagccttct tcctgccaaa gaccattcat gtacatgatt taagtcacct ctacgccaat
     2221 attttccgta aaatccaaga actataaaca gaacaaaaag accaacaaat ggaattgtgc
     2281 catcaaatcc aatcattttt gtaagaatct cctcctcttc tttctccagc ccctcagcgc
     2341 attaaatatg caaatcccaa ataaaaccca gagcaacata caagccataa tgtctgaaac
     2401 cagtaaaaga agggcattcc aaaaagttct ggcatgtttc tattgtaaag agaaactgag
     2461 agattaacta tagatggtat caaaatcaaa agagctagga taacgtatcg tgctttgttt
     2521 ttcactattt cgttaaatta aaaacataag ttaaaagcat tgacgagata atttctataa
     2581 tagatctttt gaatacatgt aaaagtcttt tactgtttct attctcttat ccaactagca
     2641 aaccccttgt tgtgcgtgac ctttctacgg taaggtcttt tgtgttgaat ggggatttgt
     2701 gttagctgct gtatatgtga gctattacga gctctgatag atgtttgcag ctttctggct
     2761 ggttggttgg ttactgatag taatagtatc acaataaaaa aaggaggtga aaatgagaat
     2821 aaaagaaaaa ataatagaat caaaaaaaga tgattactat atacattgca gtagacatag
     2881 gaaagagaaa ttgcgttgta tgtataactg ataaagatgg ttccatatta gaggaaacaa
     2941 aatatgaaaa cacattacag gaagcagggt ttgcttctca tttgaataaa aagtatagca
     3001 acagaaagtg tcatgcagtt tatgaatcaa caaacttctt gcaagcgcaa aatttcgaca
     3061 agacgcaaca atattgtcac tttttaacaa tctaaaaatt gcaatagata atcccgttac
     3121 aaatgatcct actggatatt attcaacaga tcttgttaaa gagataatgg atgaaaaaga
     3181 atcctcacca aggctatttg ttataaacct ttcggaagca gacatcgcaa gaacgttttg
     3241 ttcagaaata attaataggg tattcaggta caggaagaat agctttactc tcaatccaaa
     3301 aataatattt gtctttgacg aggcacaaga atttattcct tatgaaaaaa aagagaagat
     3361 ggaaccgaaa gttctatact gactaactaa aattcgttta caaattcttt ctgataagat
     3421 aggtaaatta tgcttgaaaa tcaacatttt tcatatatct agaatatgcg aaatttggtt
     3481 agtcagtata gtagagcact tgagaagttg ttaagacatg gacgtaagta taacctacat
     3541 ggttggatat ctactcaaag gattgcacat ttaaatacaa atgctctaca acaacttcat
     3601 agctattttg taagtacaat gccaaggcct tatgacagac aattaatctc tgatacgttt
     3661 gccatagatg atgcatttat ggacagggca ttaacctttg aaaatggaga ctggtttatg
     3721 actagtttca aggcaacaaa tacgcaaaat gtgcctgttt tctttgatgc aaaagataat
     3781 gaagattttc tatcagaaaa aataatgaaa tagatcttgg atgattcaaa atactgttat
     3841 agaatatcag taagcatatt ttggaaatta catcgttgaa tgatttctga aatgaggcga
     3901 tattttttaa acggaagaat aggacataca cacagttata gatctttcgt taaattaaga
     3961 gccttaatat ctattaaaaa ttctatattt aacaaataga tattatattt ataaaaatta
     4021 acttattggc tatttttcta ttgtatgttc ttgccaatga tttgtacata accatatatt
     4081 tt
//
LOCUS       DATIDL010000102        23823 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrosopumilaceae archaeon isolate SMAG_U16342
            k141_934061, whole genome shotgun sequence.
ACCESSION   DATIDL010000102 DATIDL010000000
VERSION     DATIDL010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935836
            Sequence Read Archive: SRR25158326
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosopumilaceae archaeon (soil metagenome)
  ORGANISM  Nitrosopumilaceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrosopumilales;
            Nitrosopumilaceae.
REFERENCE   1  (bases 1 to 23823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/04/2024 05:09:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,520
            CDSs (total)                 :: 1,474
            Genes (coding)               :: 1,474
            CDSs (with protein)          :: 1,474
            Genes (RNA)                  :: 46
            rRNAs                        :: 1 (5S)
            complete rRNAs               :: 1 (5S)
            tRNAs                        :: 43
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23823
                     /organism="Nitrosopumilaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_934061"
                     /isolate="SMAG_U16342"
                     /isolation_source="soil"
                     /db_xref="taxon:2202732"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2329)
                     /locus_tag="VLB45_04515"
     CDS             complement(<1..2329)
                     /locus_tag="VLB45_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008298648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-bladed beta-propeller"
                     /protein_id="HSD04999.1"
                     /translation="MCLLSLALIFLIPQEVYAIDITATPQKINFDPNDWLRIDISVNG
                     YLGGPVSWTAHRPDNSTFSGSLDQLRAGKTTHQIVRSAFDNYFGNWSITYTYNGVNQT
                     ASFVVKPITLSVKLDKELYYDGDIMKISLTTSYFLPTVTKAEYYHLNLYDKKGNLAKH
                     LEQIDIGAYQSSTIYNFPIDTLVQDNPIGDYKIKVKYYNVTSEIPFEVGDIEKRTTIF
                     VSTDKSLYQIGDSVDLTLIFSKVRESEGVIEITDPSGITSVKTFPVSAVSTKLHLEKI
                     ASILGKYRFEIHYAGVTGSGSFVVGTGTIKTEPDIGLELELDKQKYRPGETIKAKIHT
                     SDIIAEFASFWFEDPSGKEESKLAVSVTSGDTIVSYKINKDAPVGSWKMYVDYGGVTG
                     SAIFFVEEESGDTDIINTDSVASPQLLFTLGYDNIKFKNPRDIAVDSENNIYVVDSGN
                     SQIKKFDSNGKILASWGSFGVGEGQFKNPSGIFVDKKYVYVVDTANARIQKFDKQGNF
                     VYAWGTYGDEPGMFRTPVAVAASNSGDLFVSDSSVNRIFVFDSNGQYKDEIRSPLTLP
                     AKFSTTNYIVFDSKNNFYIIVSNENKVLQYSSIGTFLKSFGATGWKEGQFNKPDSMTI
                     DSDGNLYVTDTGNNRIQKFDSQGKFLVSWGTFGSEPGQFKDPVGIAVDSKKNVYVVDR
                     TNNNVQKFAPYDAPVDIVIPSWIKNNARWWAEDKIQDSDFAYGIQFLIKQKIINIPEA
                     KLSATDSVKIPSWVKVNAGWWSDDKISDKSFVAGIQ"
     gene            2444..3151
                     /locus_tag="VLB45_04520"
     CDS             2444..3151
                     /locus_tag="VLB45_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HSD05000.1"
                     /translation="MKVRLFEIFTSVEGEGILYGTKTLFVRLAGCPFKCFYCDTKDSL
                     PMNSGQEYSVEEACNLIEKNLEKNTYKVNFTGGDPLVQSEAVLEMAKFVKTKKIPTYL
                     ESSCYDSQKFLQILPYIDYIKIEFKTPESEFVDPQHYSKLLENELQCLNYAANSKKTT
                     YIKVVISSKTELEPFKELLEKVFSIKTEIAGFIIQPTYGIAEPSLKQLFSFYDAVYPY
                     YKNVRIIPQLHKFIGAP"
     gene            3155..3715
                     /gene="folE"
                     /locus_tag="VLB45_04525"
     CDS             3155..3715
                     /gene="folE"
                     /locus_tag="VLB45_04525"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HSD05001.1"
                     /translation="MDKERVKRLVRELIAEIGEDPSREGLIDTPNRIADMYEEIFKGY
                     DAESELSIKFSEDSDVVVTKDIKFYSMCEHHMLPFFGKVAIAYSPNGKVFGISKLVRL
                     VEKYTKRLQIQERITKNIADELYSEGVKGVIVIAEGEHFCMKMRGVKNDAKVTSIAYR
                     GIYEKKETRSDILTLIHNSSSETKLL"
     gene            3760..4014
                     /locus_tag="VLB45_04530"
     CDS             3760..4014
                     /locus_tag="VLB45_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008297052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05002.1"
                     /translation="MGVHNNVHIPKESFPTFLWYAIEFFIIVGISAGVAMITVDYFTQ
                     MGFSEIMTNWIFWGIVGGIFLVYYMIIRAFLFKKPVLSNI"
     gene            complement(4021..4695)
                     /locus_tag="VLB45_04535"
     CDS             complement(4021..4695)
                     /locus_tag="VLB45_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014966116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase"
                     /protein_id="HSD05003.1"
                     /translation="MNRAVLVFSGGLDSVCTAAYLQSRYELYGITFSYGQKANHEIRM
                     AKHFAKILRLKEHKIVNIEFMKNLYEKTNALTNSKMSIPEKFDYTIVVPIRNAVFLSI
                     AFAWAFAKNATLVAYGAHKGDKHYPDCRPNFSKTIADAFNLGEIDGIRKGIRKKIDVW
                     SPFIGGFSKSDLLKIGYKKLGDEIFKTWSCYCNTKIHCGKCESCNNRKIAFLQAKIID
                     KTKYRF"
     gene            complement(4733..5569)
                     /locus_tag="VLB45_04540"
     CDS             complement(4733..5569)
                     /locus_tag="VLB45_04540"
                     /inference="COORDINATES: protein motif:HMM:NF018506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HSD05004.1"
                     /translation="MELSDGIGLLFAVLAAVSWGTFFVPVRKVGVTNIWQLQGATSVG
                     VLLFSIPIGIFWGFEIQLGGILSGIIWTIANLLALYSVKLIGLARTSPLLAGFSIITA
                     FVWGIIFFGETFDFLWIAVSAIGLLLFGLSLVISKEKNQVIQKKGYAIAIASGLVGGS
                     YVIPMQATQTLQSGFFSLSISIFAIGIPMFFLAKRFITKETIAGIASGVLFNTGSLSV
                     LVAISLIGITTAYPISQTATLFAVSWGILYFKEVVQKRRIFQISIGAIIILCGAALLV
                     IA"
     gene            5661..6419
                     /locus_tag="VLB45_04545"
     CDS             5661..6419
                     /locus_tag="VLB45_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008297871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HSD05005.1"
                     /translation="MSPQYLQVEKDDQIALITINRPEKLNAMNLQVMDEFISVLDDLE
                     KDQSKVIIITGAGQKAFSAGADIEYMSKIGSTEAEKYALRGHEVLNKIEKIEKPVIAA
                     INGYALGGGCELGLACDIRFASPNAQLGQPEVTIGICPGWGGTQRLLRIIGPSKAKDL
                     IFTGRKIGAQEALDMGLINKIIPVENLIAESKAYAKNISKNSSFAVGISKMLVNKGMD
                     ADLDTGLKLEIYSWSLCFSSKDREERMRAFVEKK"
     gene            complement(6420..7337)
                     /locus_tag="VLB45_04550"
     CDS             complement(6420..7337)
                     /locus_tag="VLB45_04550"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010191782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006566 - threonine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HSD05006.1"
                     /translation="MASSVVVKAPSSTANLGPGFDVFGLALDAYYDQIRLEKSSDGIR
                     IYSSSPIPLVPEKNSAGLVVKEMAKKFKIKSGLVLKINKGVPAGFGLGSSAASAAAAA
                     VAFDALFGLNLEPNRLVEFAGIGEKASAGTIHYDNVAASTLGGFVIVRTNPLDVIRIE
                     PPKDLVLCIAVPKLAVPVKKTQVSRGVLPKQIKLSDSVQNLSNAAAIVAGFMKKDTTL
                     IGSSIRDVIVEPARQHLIPGFIQVKENAIDAGALGVTISGAGPSVIAFASKSSNTKKI
                     CSAMASGFASAKTKCDTIICRPSKGATVV"
     gene            complement(7338..8096)
                     /locus_tag="VLB45_04555"
     CDS             complement(7338..8096)
                     /locus_tag="VLB45_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HSD05007.1"
                     /translation="MKTIFVIGTAGAGKSSLASKIFDYYTRNGSFVGMLNLDPGVENL
                     PYTCDVDVRDYIDIVSIMRQYDLGPNGALIMANDLIASKIDDLQHDVDNVNPDYLIVD
                     TPGQIELFAYRTSGPFFVQNFISEQKVALFLYDGVMITNPLNFVSVALLATSIKLRLG
                     LPNVNVLTKTDLIEDRIGDILRWSTNLKILEDAIAKQSDGESYTLVTNILRGLNIGGF
                     SQGLIPISNATGNGMVNLEAALSRILNLGEEVED"
     gene            8154..9170
                     /locus_tag="VLB45_04560"
     CDS             8154..9170
                     /locus_tag="VLB45_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HSD05008.1"
                     /translation="MNWKLLAIPVSIIPFIFIAFTFDIKLEELFAVGLFPFAAAALAI
                     SVKLLTQGLKFNYLVKKFLGSIEAPWRVVSARIGSEFVTSTTPSFVGGELVRIIWLKK
                     RGVPTGKATWITIMEIVTEVLVAGIFALIAATFAFVNGAYVIGMAIVAISLPVTALWS
                     TLFFLSYKRTFQVPIWMSRIAERFGKERASKYVEKTNQWMLEICDMSRQSLHNKEAKK
                     AFVVSMIFSVFTWIFYGISFMVIANGAGYFIGFFDSILATMASNAIGNLPITIGGSGL
                     TEFAIWAYLGHLNSLTFEAAEDSIQWNIVIAWRIATYHVSLVISWILLMKIVYPRTKK
                     AEIA"
     gene            9206..9997
                     /locus_tag="VLB45_04565"
     CDS             9206..9997
                     /locus_tag="VLB45_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007551743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HSD05009.1"
                     /translation="MDFENKVVVITGGSSGIGEASAMEFAKRKCTVILVSRRKDRLDD
                     VAKKLAKHNSETLVCQCDVSQKKEVEQMSKTVLDKFGRIDILVNNAGFAIYGSVKDLK
                     IEEIESQMTTNYLGMVYCTKAFLPKMLEQKSGHIVNVASVAASFGLPGIASYCASKFA
                     MLGFSEGLHHELKGTGVGVTVVSPIMVKTNFFDHPSFKSMPKYSPTSLSPKTVAKAVV
                     RASSSPRLEIIIPSFVRGAVWMKHTIPYLLNPILGKAFRDKMNSK"
     gene            complement(10047..10439)
                     /locus_tag="VLB45_04570"
     CDS             complement(10047..10439)
                     /locus_tag="VLB45_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016940143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05010.1"
                     /translation="MAKELPKWAQDEIKSVKFGKPETQNRTGYILEIYDKDMKIDAQL
                     YQEVDDGRRIITLDLPKKIKPAELLKGEVYEFTFNSAKAPLSKKLVDLLKKEMEIDMD
                     AIYQFELVNLELMDVGSDTSAPAESEEE"
     gene            complement(10457..10678)
                     /locus_tag="VLB45_04575"
     CDS             complement(10457..10678)
                     /locus_tag="VLB45_04575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05011.1"
                     /translation="MTDCPECAAKEVVIEMKFDLNTKHFICKKCGLYATREQISDIRD
                     RLNRRERTREDKHDEYRDWWQSSKKEKQN"
     gene            complement(10825..12054)
                     /locus_tag="VLB45_04580"
     CDS             complement(10825..12054)
                     /locus_tag="VLB45_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HSD05012.1"
                     /translation="MIKLGQFIYPWGNGHYSRMMALDSILPSYIKDEFEMHYSSKGEI
                     YQRLLQKFPQKKKQIHEILMPTPIDGKHGPSVTLSMLNLLLPVAGNPPLVKQISSYLK
                     NEASLYNKEKFDLVINDGDMGSNVLAERRKIDSVFVTNQFKPRLWRSHFYFYPSLIYI
                     SKQIAKATKIVVADSAPPNTICEYNLNFPEEIKHKVVYAGHFSTDILTDKPKSDLEKL
                     IENSETFGYWMQTGNKSTKEATARKYEQAFHSDELKNEKRLVSHAQNDASLDMVKGKD
                     GKTYSISDALEQKIDWIQIDVGFLSEQEKNTVLSLCKYAVINGSHTAMGEIIGMKAKP
                     IIGIPVYDEHTNQIRWAEDKKLGVLANNTKQVISAVQNIRNNYNKYEERLTEFTKNFD
                     RNGANNTARIIAEMLDN"
     gene            complement(12087..13223)
                     /locus_tag="VLB45_04585"
     CDS             complement(12087..13223)
                     /locus_tag="VLB45_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014966122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine biosynthesis protein"
                     /protein_id="HSD05013.1"
                     /translation="MSEKTVVVVFPSLFSLNKINSLASSISAALKAKRQSFSKIEKKD
                     SVIIVEAKDPVFASTTINSLFGIEKVAIAIEVENNFNSVVSAIAKAGVNLVLSGEKFY
                     VKVDGKTSTYMPKDVELAATSLLIEKTVNLQAKPGTEKNHDKMIYTYLTKSYAYVCIF
                     MDKGKGGIPYKSQKETILCCLYDELSIISCLQIIKMGFDVKILVCYRNESELLNTVKM
                     MNHILPIISQTKIELGFYKLDMKFSGNNGLLFRIAVVTEILLSVATKAKIDRVSLSIS
                     PLIFPVWFVEHNITRVFQKGKIPWMPLSGIDDSIFETANEIGLRKYVSKIENLCRLKF
                     STKSIPKEKIYKISQATLHSQKIITIPIGPKNIHDIVDSLRSNH"
     gene            13316..14038
                     /locus_tag="VLB45_04590"
     CDS             13316..14038
                     /locus_tag="VLB45_04590"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016939450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HSD05014.1"
                     /translation="MGKFTAEQLSQINNTLKTPQETLKWALDNLHPKLGFASSFGAED
                     VVVIDMLMKINPKARIFTLDTGRLNQETYDVMDEIRNKYNMNIEVMFPDQNEVEQMVK
                     VNGMNLFYHSMGNRKLCCGIRKVHPLNKMLATLDGWITGLRSDQTQNRSDAKRIEIDE
                     QHNNMVKINPIIDWKWEQVWDYVKQNKIPYNKLHDKGYPSIGCEPCTRAIKPGEDLRA
                     GRWWWETDPEKECGLHADHGNK"
     gene            14043..15188
                     /gene="sat"
                     /locus_tag="VLB45_04595"
     CDS             14043..15188
                     /gene="sat"
                     /locus_tag="VLB45_04595"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014966124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase"
                     /protein_id="HSD05015.1"
                     /translation="MTESKGVAIPHGGKLVNRFTTKNTDDMFSVSVDTDLANDIENIA
                     DGIFSPLEGFLSQQDFESVVKNGRLSNNLPWTIPIVFDADKQTASKMKDSRDVLLKNS
                     ENNGFAILHVEDAYSFDKNTTAKGIYGTTDPKHPGVAKTMAMQDFLIGGKIDYVKRPK
                     EIPIRKYRKTPTETRANFEKAGWKTIVAFQTRNVPHVAHEMLQKASLNTHDGLFVNPL
                     IGKKKSGDFTDEVIVSAYEILIKHYYPENRCSLATLHTEMRYAGPKEAIHHAIMRKNY
                     GCTHIIIGRDHAGVGNYYTPFAAQEIFDEYQDLQIEPIFYPAFFYCKKCLSFASEKNC
                     PHGTEFQEQLSGTKLRTMILEGQTPSEYMIRPEVSKIIMNWNKPFVD"
     gene            15270..15887
                     /locus_tag="VLB45_04600"
     CDS             15270..15887
                     /locus_tag="VLB45_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05016.1"
                     /translation="MYAQTASNPTVRLESIDIPFEKFNVISSNSIMGEFEHEHSINWQ
                     LSIKNHIVYANPLGNAVIQLYDGVDKEKFIEIGMGSPPDRKFWVAVQNPEIGYAVVHD
                     QTKDGWNPNDEIVVVYSSTADLTINNGQRIVVSNLDVGNFEIKDFSVYGMESSTDPPA
                     TNSGNLILNMVSGNPAANPLFYLPYILAIGAGALVVVLLKTKKRT"
     gene            complement(15871..16524)
                     /gene="nth"
                     /locus_tag="VLB45_04605"
     CDS             complement(15871..16524)
                     /gene="nth"
                     /locus_tag="VLB45_04605"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014966126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HSD05017.1"
                     /translation="MEKILRGMINTMNSVKPPRMTALRELREAESGDPLSILIGTILS
                     ARTRDENTSTVVRKLFAKYKTARLLARAKISDVERIIKSTGFYHVKARRIIEVASLID
                     SQYSGKVPDSLDELLKLPGVGRKTANCVLVYAFDKPAVPVDTHVHRISNRLGLVETKT
                     PEETEFALMEKVPKKYWLKINDVFVMYGQNICKPISPMCSVCQIKKECNYYKTRFSS"
     gene            16595..17749
                     /locus_tag="VLB45_04610"
     CDS             16595..17749
                     /locus_tag="VLB45_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase X catalytic domain-containing protein"
                     /protein_id="HSD05018.1"
                     /translation="MDLTKDEESALKGEQGETLALAYRILVAIGEATNADRLIPIEWA
                     HLSGVNYNTIGDSGEEFLSKLSKNAKFKVKTTVNPMGIDFDNVSKYDVDEEFINKQKS
                     IKSSYEKMGAITSFSCIPYEIFDLPKKNTHVSFAESNAAIYVNSVSDLKTNKESAFSA
                     LASALTGKSPYSDLRKDESRDPKITIQMKVDQKDDLIYGMLGYFAGKVANSSVAFSGI
                     NKLDKRSCKSLCAGMGTSGTCGMFTLDGNKGEKVDFDKKEAQKIHDELNTSESGDIIA
                     LGSPQLGLDELSDLAGMLKGRSFNKQCMIFCPRVVQEQARRIGYANEIERAGGELLSD
                     CCICLTPLINKKNVDGVVTNSVKGAYYLKTSNGVDVNLKSLNQIVREETK"
     gene            17746..18129
                     /locus_tag="VLB45_04615"
     CDS             17746..18129
                     /locus_tag="VLB45_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014966129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF126 domain-containing protein"
                     /protein_id="HSD05019.1"
                     /translation="MTLKIIVKGKVEGEVMVAKNPINFLGAVDKKTGKIRDEKHDLFG
                     KSLAEKILVFPFGVGSSVGAYTIYSLKFNKCAPQAMICLKADITTASGCALANIPMVI
                     ATREDYDSLIDGKKILLDADNGKIL"
     gene            complement(18124..18723)
                     /locus_tag="VLB45_04620"
     CDS             complement(18124..18723)
                     /locus_tag="VLB45_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00296 family protein"
                     /protein_id="HSD05020.1"
                     /translation="MSLSDQDGESLVKTARLAVTEYLKTGKKIILPEEFKSKFSYNSG
                     VFVTLNKAENLRGCIGFPTPEKKLYQSLVDAAIASSTEDPRFPKVKYEELRDISFEVT
                     VLTPPLEIKVEKPLEYPSKIKTGRDGLIVKWEFGSGLLLPQVPVEYGWNEEEFLNHTC
                     EKAGAPQDCWKNKSTKILKFEGIVFKEVSPDGDVVRILL"
     gene            complement(18720..19208)
                     /locus_tag="VLB45_04625"
     CDS             complement(18720..19208)
                     /locus_tag="VLB45_04625"
                     /inference="COORDINATES: protein motif:HMM:NF014037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mut7-C RNAse domain-containing protein"
                     /protein_id="HSD05021.1"
                     /translation="MSEKRPIFVIDSMLGNLAKKLRILGYDSTFYSDIEDEKLVIIAK
                     NEKRVLVTKDEQLTKSADKQGIMTILIRGNDELEQIAQINAKIRLEKIFVDTDNSRCT
                     VCNGKLQAIEKYRIINKIPEGILEREEKFWMCESCKKIYWKGTHFKKLQEFADKLNNR
                     LQ"
     gene            complement(19205..21136)
                     /gene="rqcH"
                     /locus_tag="VLB45_04630"
     CDS             complement(19205..21136)
                     /gene="rqcH"
                     /locus_tag="VLB45_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010194405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome rescue protein RqcH"
                     /protein_id="HSD05022.1"
                     /translation="MTLAGIELRYLVNEISKKTEEYYVSNIYGISRDSLLFKLHHPEK
                     PDILLMFSTLGTWITTVKIDQIEENRLVKRLRNDLLRLRISEIKQIGAERIAYITFSG
                     LEQEFVLVGEFFGDGNIILCDKNMKILALLHSINVRHRELNLGLEYQPPPQSGLDIFN
                     ITSKELSEVLSITTPVVRWIGRTLGLPTRYAEEIIRLAKIDPQATGNSLSEKDIDMIF
                     TAIKSLTEKIVNGIHEPVLVRTEKSVDVLPIALGQTENANCTKITSFMEGLDSLFTEN
                     LLEHGKSTQANTVNQKIEEFEHKLEEQIKAISAVKEKASKISNIAKSLMEMVSSGITT
                     LDSPTILEMLVNKHSSFVKERGILFIDIDGEKIKIEPDSSIPAVASVLFNESKRQLRA
                     IELIESAKKQTEKSLDKFRKQASIAEDSVVFTVQKKREWYERYRWFFTSDGLLAIGGR
                     DASSNSSVIRKHLEKDDKVFHAEIFGSPFFILKNSNEINVSSIMETAQATVCFSRAWR
                     EGLYGLNSYWVHPDQVKTAAPSGQFIAKGSFVIDGQRNFVKVTNMRLCVGLLEKNEKY
                     SLMCGPPEPVKKNSIYYVIIEPSGSEMVEIAKKIKLEFLKFKEKEEIVKTISIDDFIR
                     VLPAGESHIVESGEGEANK"
     gene            21236..21661
                     /locus_tag="VLB45_04635"
     CDS             21236..21661
                     /locus_tag="VLB45_04635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05023.1"
                     /translation="MTRHPKKKENEKKSESGNPIDNPPQSEPNTNQTEGIKMKDPLNK
                     LFWIRVALGILAGTISAQVGSSVDMSSRAFVGFGIMIILFIVSYGIAKAMHLPLPPSD
                     RKKLIMTGIGSYFFMFIFTWILVNTLLHPAAVTSLNPIK"
     gene            21694..22284
                     /gene="cbiT"
                     /locus_tag="VLB45_04640"
     CDS             21694..22284
                     /gene="cbiT"
                     /locus_tag="VLB45_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046025 - precorrin-6Y
                     C5,15-methyltransferase (decarboxylating) activity
                     [Evidence IEA];
                     GO_process: GO:0046140 - corrin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-6Y C5,15-methyltransferase
                     (decarboxylating) subunit CbiT"
                     /protein_id="HSD05024.1"
                     /translation="MWKYETPGIPDEFFERVEEVPITKEEVRAVQISKARLSTGQTVF
                     DIGCGSGSISVEAALQVKSSGKVYSVDFDPKAIELTKKNLAKFQITNVIVTLGNAIDK
                     ISELPMADAIFIGGTGGDTKDILKLCEGKLKSGGRIVIGTILVETLVAVLEVIDKLKF
                     SSVDVTQITVSKSRKTSTGTMMLARNPVTIISATKI"
     gene            22317..23039
                     /gene="cobI"
                     /locus_tag="VLB45_04645"
     CDS             22317..23039
                     /gene="cobI"
                     /locus_tag="VLB45_04645"
                     /EC_number="2.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010191484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030788 - precorrin-2
                     C20-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-2 C(20)-methyltransferase"
                     /protein_id="HSD05025.1"
                     /translation="MAELTCVGCGPGDPDLLTVKAVKAIRNADVIMCPTSKEGKTSIA
                     YSIISSVIDGSKKPEIINLVFPMVKDRETLEDTWEKNAKAIASAIKSGKNVVYLTVGD
                     PSLYSTWIYLQRELKSKYPEIKLTVIPGIVSMFAFASKVGISIAEGDETVAVIPACYD
                     LSRVKETAKNCDTMIFLKDGRYFDQVITLLKEAGFPDNSIFAIAQDVGTEEEIVKKLT
                     LGEVTKDTLTSKYFSIMVVKRA"
     gene            23032..23796
                     /gene="cobM"
                     /locus_tag="VLB45_04650"
     CDS             23032..23796
                     /gene="cobM"
                     /locus_tag="VLB45_04650"
                     /EC_number="2.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0046026 - precorrin-4
                     C11-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-4 C(11)-methyltransferase"
                     /protein_id="HSD05026.1"
                     /translation="MHKVYFVGCGPGDPELLTVKAKKLIQKADVVVYSGSLIPTQIIN
                     LCKKEKYDAAKLVREEIFDILKTNTKKGKLAVRLHDGDPSIYGAIREQTDNLRKAGIE
                     FEIVPGVTSFLASAAVLGSELTLPGITQTIIITRAEARTKVPKREKISELAKHKSTMI
                     FYLSAHLLSDIVKEVISGGYPKSTPAAAVYRASWNDEKIIVGTLKDITKKVRDAKITR
                     TAIIIIGDIVKPKSYEYSKLYDKTFSHGFRKAKIKS"
BASE COUNT         7692 a         4275 c         4109 g         7747 t
ORIGIN      
        1 attgaatacc tgctacaaaa cttttatctg atattttgtc atcagaccac catcctgcat
       61 taactttgac ccatgatgga attttaacag aatctgtcgc tgacagctta gcttcaggaa
      121 tgttgattat tttttgtttt atcaaaaact gtataccata tgcaaagtca gaatcttgga
      181 ttttgtcttc cgcccaccac cgtgcattgt ttttgatcca gcttggtatc acaatgtcca
      241 caggcgcatc atagggcgca aatttttgaa cattgttatt tgttctatct actacataca
      301 catttttttt ggaatctact gctattccaa ctggatcctt gaactgtcct ggttcacttc
      361 cgaaagtacc ccaactaact aggaattttc cttgcgaatc aaatttctgt attctgttgt
      421 tacctgtatc tgtaacgtag aggtttccat cagaatctat tgtcatagag tctggcttgt
      481 taaactggcc ttctttccac cctgtcgctc caaatgattt gagaaatgta cctatactac
      541 tatactgcaa aaccttattc tcattagaaa ctatgatgta aaaattattt ttagaatcaa
      601 aaactatgta gtttgttgta gaaaattttg ctggtaaagt aagcggtgat ctaatctcat
      661 ctttatattg accattcgaa tcaaatacaa aaatcctatt tacgcttgaa tctgacacaa
      721 aaagatctcc tgaattagag gcagccactg ctactggtgt acggaacatc cccggttcat
      781 ctccatatgt tccccatgca tatacaaagt tcccctgctt gtcaaatttc tggattctag
      841 cgtttgcagt atctactacg tatacgtatt tcttatcaac aaaaataccg gatgggtttt
      901 taaattgacc ctcaccgaca ccaaaagatc cccatgatgc aagaatcttt ccgttagaat
      961 caaatttttt aatttgtgaa tttccagaat ctactacgta gatattgttc tccgaatcta
     1021 ctgcaatatc tctcgggttc ttgaatttga tattatcata accaagagta aaaagtaatt
     1081 gtggagaagc aacagaatca gtattgataa tatctgtatc acctgattcc tcctctacaa
     1141 agaatatggc agatcctgta actccgccat aatcaacata cattttccat gatccaactg
     1201 gcgcatcctt gtttattttg taagaaacaa tggtgtctcc agatgtcaca gatactgcga
     1261 gtttagattc ttctttacca gatgggtctt caaaccaaaa acttgcaaat tctgcaatta
     1321 tgtcgctagt gtgaattttt gcttttattg tttcacctgg tctatacttt tgcttatcaa
     1381 gctcaagttc taaacctatg tctggttctg ttttaatagt tccagtccca acaacgaaag
     1441 aacccgaccc tgtaactcct gcataatgta tctcaaatct atattttcca agtattgatg
     1501 caatcttctc aaggtgtagt tttgttgaca cggcacttac tggaaatgtt ttgactgatg
     1561 taatgcctga tggatctgta atttctatca ctccttctga ctcccttacc ttagaaaata
     1621 tcagtgttag atcaacacta tcacctattt ggtaaagcga tttgtctgtg ctgacaaaaa
     1681 ttgttgtacg tttttcaata tctccaacct caaatggaat ctctgaggta acattgtaat
     1741 attttacttt aattttataa tctcctatag gattatcctg aactagtgta tctattggaa
     1801 aattatagat tgtacttgat tgatatgcac caatgtctat ctgttcaagg tgttttgcaa
     1861 gatttccttt cttatcatac aaattcaagt gataatattc agcttttgta acagtaggaa
     1921 gaaagtatga tgtagttaag gatattttca taatatcgcc atcatagtag agttccttat
     1981 cgagttttac agaaagtgtg attggcttaa ctacaaacga agcggtctga tttacaccat
     2041 tgtaagtata cgttattgac caatttccaa aataattatc aaatgcactt ctaacaattt
     2101 gatgagttgt ctttccagct cgtaactgat caagtgagcc agaaaatgta gaattgtcag
     2161 gacgatgtgc tgtccaacta acaggaccgc caagataccc atttaccgaa atgtctattc
     2221 tgagccagtc gtttggatca aaattgattt tttgaggtgt tgctgtaata tcaattgcat
     2281 agacttcttg tggtatgaga aaaattaagg ctagtgagag caaacacaag cagagtaaga
     2341 ttttcacaat ttctgtctgt tttgtgtgaa taaatcgttt tagtaacaat tattcatata
     2401 acaatccaat tgaataaata ttgaaaaact gaatattgtt tacttgaaag ttagactatt
     2461 tgaaatattt acttctgtag aaggagaggg tatactatac ggaacaaaaa ctctttttgt
     2521 aaggctggca ggatgtcctt tcaagtgttt ttactgtgac actaaagatt cattgccaat
     2581 gaattcagga caggaatatt ctgttgaaga agcctgtaac cttatagaaa aaaatctaga
     2641 aaaaaataca tacaaagtaa attttactgg tggagatccc cttgtacaat cagaagcagt
     2701 tttagagatg gcaaagtttg ttaagacaaa aaaaattcct acttatctag aatcgtcatg
     2761 ctacgactct caaaaatttc tccaaatact tccgtacatt gattacatca aaattgaatt
     2821 caaaacccca gaatcagagt ttgttgatcc acaacattat tctaaacttt tagaaaatga
     2881 acttcaatgt cttaactatg cagccaactc gaaaaaaacc acttacatca aagttgttat
     2941 aagctcaaaa acagaacttg aaccattcaa agaactactt gagaaagttt ttagcatcaa
     3001 gacagagatt gcaggattta taatacagcc aacctatggt attgcagaac ccagtttgaa
     3061 acagctcttt agcttttacg atgcagttta tccttattat aaaaatgtac gaataatacc
     3121 acaacttcat aaattcatag gagcaccata aatgatggac aaagaaagag taaagcgtct
     3181 agtacgggaa ttgatagcag aaataggcga ggatccttca cgcgagggtc taatagatac
     3241 cccaaatcgt attgcagaca tgtatgaaga aattttcaaa ggatatgacg ctgaatctga
     3301 actctccata aaattttccg aagattctga tgttgttgta acaaaagata ttaaatttta
     3361 ttcaatgtgt gaacatcaca tgctgccttt ctttggtaag gttgcaattg catattcacc
     3421 aaatggaaaa gtttttggaa tttcaaaatt ggttagactt gtagaaaaat atactaaaag
     3481 attgcaaata caagaaagga tcacaaaaaa cattgctgat gaactatact cagaaggcgt
     3541 taaaggagta attgtaatag ctgaaggtga acacttctgc atgaaaatgc gtggtgtcaa
     3601 aaatgatgcc aaggttactt ccatagcata tagaggaatc tatgagaaaa aggaaactag
     3661 atccgatata ctgacattaa ttcataatag cagctctgaa acaaaactac tttaaaattt
     3721 ttgaaaaaac taaataatag cgtctaaatc acgacaaaca tgggagtaca caataatgtg
     3781 catattccaa aagaatcatt tcctacattc ttgtggtatg ctatagaatt tttcataatt
     3841 gtaggcatct ctgcaggagt tgctatgatt acagtagatt attttacaca aatgggattc
     3901 tctgagatca tgacaaactg gatattctgg ggcatagttg gtggaatttt ccttgtatat
     3961 tatatgataa tacgagcatt tctattcaaa aaaccagttt tatcaaatat ctgaatattc
     4021 ttaaaaccgg tatttcgttt tatcaattat tttggcctgt aaaaaagcaa tttttctatt
     4081 attacaagac tcacatttcc cacaatgaat ttttgtattg caataacaac tccatgtttt
     4141 gaagatttca tctccaagtt ttttatatcc aatttttaat aaatcacttt ttgaaaaacc
     4201 acctataaaa ggactccaga catcgatttt tttcctaata ccttttctta ttccgtcaat
     4261 ttcacccaag ttaaatgcgt ctgcgatagt tttggaaaaa ttgggcctgc aatctggata
     4321 atgcttatcg cctttatgtg ctccataagc aacaagtgta gcattttttg caaaagccca
     4381 tgcaaaagca attgaaagaa aaactgcatt tcgtataggt acgacaatag tataatcgaa
     4441 tttttcagga atgctcattt tagagttggt aagggcattt gttttttcat acaggttttt
     4501 cataaattca atatttacta ttttgtgctc ttttaatcgc aaaatctttg caaagtgttt
     4561 tgccattcta atttcatgat tagccttttg accatatgaa aaagtaatgc cataaagttc
     4621 gtatcttgat tgtagatatg ctgctgtaca tacagaatca agaccaccgc tgaaaaccaa
     4681 tactgctctg ttcattttgg attttctgtc ttatgccaca taaattgttt gattaagcaa
     4741 ttacaagcag tgctgcaccg catagtatta tgattgctcc aatggatatt tggaagatcc
     4801 ttcttttttg gacaacttcc ttgaaataca aaattcccca tgagacagca aaaagagtcg
     4861 ccgtttgaga tatcggatat gcagtagtga tgcctatcag acttatagca acaagtacag
     4921 acaaactgcc tgtattgaat aaaacacccg aagctatgcc agcaatagtt tcttttgtga
     4981 taaatctttt tgcaagaaaa aacattggta tcccaattgc aaaaatggaa atactaagag
     5041 aaaaaaatcc tgattgaagt gtttgagttg cctgcattgg tatgacatat gatcctccta
     5101 ccaaacctga agcaattgct atcgcatatc cttttttttg tattacttga tttttttctt
     5161 tgcttattac aagtgataac ccaaaaagca ataatcctat ggcgcttact gcgatccata
     5221 aaaaatcaaa tgtttctcca aaaaatataa ttccccatac aaatgctgtt atgatactaa
     5281 aacctgcaag cagaggcgat gttctagcaa gtccgattaa cttgaccgag taaagggcta
     5341 acaaattagc tattgtccag ataattcctg atagaatacc acctagttga atttcaaaac
     5401 cccaaaaaat accaattgga atagaaaaaa gcaacacacc aactgaagtt gctccctgaa
     5461 gttgccaaat gtttgtaacg ccaacttttc taacaggaac aaaaaatgtt ccccaactta
     5521 cggctgcaag tacagcaaaa agtaacccga taccatcaga caattccatt ctacatcaat
     5581 ttgcatgaaa atatgtgtgc ctactctgat attggtgaca agcaactaaa gtttaatttc
     5641 tagaaaaaat agtttctgtc atgtcaccac aataccttca agtggaaaaa gatgatcaaa
     5701 tagctctgat cactataaac aggccagaaa aacttaatgc aatgaatctt caggttatgg
     5761 atgaatttat ttcagtatta gatgatctgg aaaaagatca atccaaagtg attataatta
     5821 ctggtgctgg tcaaaaggca ttttcagcag gtgctgacat tgaatacatg agtaagatag
     5881 gatctactga agcagaaaaa tatgcattaa gaggacatga ggtactaaac aagatcgaga
     5941 aaattgagaa accagtcatt gcagcaataa acggatatgc tcttggtggt ggttgtgaac
     6001 ttggtcttgc gtgcgatatt agatttgctt ctccaaatgc acagcttggt caacccgagg
     6061 ttacaatagg gatctgccct ggatggggtg gcacacaaag acttttacga attataggac
     6121 cttccaaagc aaaagatttg atctttacag gacgcaaaat tggggcacaa gaagctcttg
     6181 atatgggatt aataaacaaa ataattccag tagaaaatct aattgctgaa tcaaaggcat
     6241 atgcaaaaaa catatcaaaa aatagcagtt ttgcagtcgg tatttcaaaa atgcttgtga
     6301 acaaaggaat ggatgcagat cttgatactg gcttgaaact tgaaatttat tcatggtctc
     6361 tttgcttttc tagcaaagat agagaagaaa gaatgcgcgc ctttgtagaa aaaaaataat
     6421 tataccacgg tagcgccctt acttggtcta caaattattg tatcgcattt tgtttttgct
     6481 gatgcaaatc cacttgccat ggcactacag atcttttttg tattcgatga ctttgatgca
     6541 aatgctataa cagatggacc tgcaccactt attgtaactc ctaacgcacc agcatcaatt
     6601 gcgttttctt ttacttgaat aaaaccggga atcaagtgtt gtctagcagg ttcaactata
     6661 acgtctctga tagaacttcc aatcaatgtt gtatcctttt tcataaatcc agcaacaata
     6721 gcagctgcat ttgaaagatt ttgtaccgaa tctgaaagct tgatctgctt tggcaaaaca
     6781 cctcttgaca cttgagtctt tttcactgga acagctagct tgggaaccgc aatacaaagt
     6841 acaagatctt ttgggggttc tattcttatg acatccaatg ggtttgttct cactataaca
     6901 aaaccaccaa gagttgatgc tgccacatta tcataatgga tagtaccagc actggctttt
     6961 tctccaattc ctgcaaactc tacaagtcta tttggctcta aatttagacc aaataaagca
     7021 tcaaacgcaa cagctgcagc agctgcagaa gcagcactgc ttcccaatcc aaagccagct
     7081 ggtactcctt tgttaatttt taaaacaagt ccagatttta ttttaaattt ctttgccatc
     7141 tcctttacaa ctaatcctgc agagtttttt tcaggaacaa gaggaatcgg gctggaactg
     7201 taaattctaa tcccatccga acttttttcc aatcgtatct gatcataata ggcatctaac
     7261 gctaatccaa aaacatcaaa accgggaccc agatttgcag tggaggaagg cgcctttaca
     7321 acaacactcg aagccaatta gtcttctact tcctctccca agttaagaat tctgctcaag
     7381 gctgcttcta aatttaccat tccatttcct gttgcatttg aaattggaat cagtccctga
     7441 gaaaaacctc caatattcaa gcctcttaaa atattggtta caagcgtata actctcacca
     7501 tctgactgtt ttgcaattgc atcctctaat attttcagat tagttgacca tctcaatatg
     7561 tctccaatcc tgtcttcgat tagatctgtt tttgttaaca cattgacgtt cggtaatcca
     7621 agtctgagtt taattgatgt agctaaaagt gccactgaaa caaaattaag tggatttgtt
     7681 atcattacac catcgtatag aaataatgct actttttgct ccgagataaa attctgtacg
     7741 aaaaaaggac cacttgttct gtaagcaaaa agctctattt gtccaggcgt gtcaacgatg
     7801 agatagtctg gatttacgtt atcgacatca tgttgcagat catctatttt tgacgcaatt
     7861 agatcatttg ccattattag agcaccgttt ggacccagat cgtactgtct cattattgat
     7921 actatgtcta tgtaatccct gacatcaaca tcacaagtat atggaagatt ttcaacacct
     7981 ggatccaaat ttaacatccc aacaaaggat ccattacgtg tataataatc aaaaattttt
     8041 gatgcaagtg acgattttcc agcgccggca gtaccgatga caaaaatagt tttcaatttt
     8101 atcgattata cttcatgttc agcttatatt tacatccatg gaaaggtaga caaatgaatt
     8161 ggaaactatt agcaattcca gtcagtatca taccatttat ttttattgca tttacatttg
     8221 acataaaact tgaagaactt tttgcagtag gtctttttcc ttttgcagct gcagcattag
     8281 caatttccgt caaacttctc acacaaggct taaaattcaa ttatttagtt aagaaatttc
     8341 ttggtagtat agaggccccg tggagagttg tctcagcaag aataggaagt gaatttgtta
     8401 catctacaac accatcattt gttggtggtg aattggtccg aattatttgg ttaaaaaaaa
     8461 gaggtgtccc tacaggcaag gcaacttgga tcaccattat ggaaattgtg acagaagttc
     8521 tagttgcagg aatttttgct ctaattgcag caacatttgc atttgttaac ggtgcgtatg
     8581 ttataggaat ggctatagtg gcaataagct tgccagttac agctttatgg tcaactctgt
     8641 tttttttatc atacaaaaga acatttcaag ttcctatctg gatgtcaaga attgcagaaa
     8701 gatttggcaa agaacgtgct tcaaaatatg ttgagaaaac aaaccagtgg atgttagaaa
     8761 tatgcgatat gagtaggcaa agtttgcata ataaagaagc aaaaaaagca ttcgtagtat
     8821 ctatgatctt ttctgtattt acctggattt tttatggaat ttcatttatg gtaattgcaa
     8881 atggtgctgg atatttcata ggcttttttg actctatact cgcaactatg gcttcaaatg
     8941 caattggaaa tttgccaatt acaattggcg ggtctggtct tacagaattt gcaatatggg
     9001 cttatcttgg tcatctaaac agtctaactt ttgaagctgc tgaagatagc atccaatgga
     9061 acattgtgat agcgtggaga attgccacat atcatgtgag tcttgttata agctggatac
     9121 tgctaatgaa aatagtttac ccacgcacaa aaaaagcaga aattgcatag aaagcacttt
     9181 attttcaaac aacacttttt ctttattgga ttttgaaaac aaagttgttg taattacagg
     9241 aggatcaagt ggcattggcg aagcgtcagc catggaattt gctaagcgaa agtgcacagt
     9301 aatacttgta tcaagaagaa aggacagact tgatgatgtg gcaaaaaaac ttgccaaaca
     9361 caattctgag acacttgtat gccaatgtga tgtatctcaa aaaaaagaag tagaacaaat
     9421 gagtaagact gtattagata aatttggccg tattgatatt ctagttaata atgcaggctt
     9481 tgcaatttat ggatctgtaa aagatctgaa aattgaagaa atagaatcac aaatgaccac
     9541 caattatctt ggtatggtgt attgcacaaa ggccttcttg ccaaaaatgc ttgagcaaaa
     9601 atctgggcat atagtaaatg tggcatcggt tgcagctagc tttggtcttc caggaattgc
     9661 ttcgtattgc gcatcaaaat ttgctatgct agggttttca gagggattac accatgaact
     9721 caagggaact ggtgtaggtg tgaccgtggt tagtccaatc atggttaaaa caaacttttt
     9781 tgatcatccc tctttcaaat caatgccaaa atattctcct acttccctta gtccaaagac
     9841 tgtagcaaaa gctgtagtaa gggcatcttc ttcaccaaga ctagagatca tcataccttc
     9901 atttgtcagg ggtgcagtgt ggatgaaaca tacaattcca tacttattga atcctatttt
     9961 gggaaaagct tttagagaca aaatgaattc aaaatgaaaa ccacaatcat ctgattataa
    10021 caaaaattat tttaataaat tgtagtttat tcttcttcag attctgcggg tgcagaagtg
    10081 tcagaaccca catccattaa ctcaagatta acaagctcaa actggtatat cgcatccatg
    10141 tctatttcca tttctttttt taataaatcc acaagttttt tgcttaaagg tgcttttgca
    10201 gaattaaatg tgaattcata cacttcacct ttcaataatt cagccggttt gatcttcttt
    10261 ggtaaatcta aagtaattat gcgtcttcca tcatctactt cttgatacag ctgagcatca
    10321 atcttcatgt ctttatcata aatctcaagt atgtagcctg ttctattctg tgtttcaggt
    10381 ttaccaaact ttacactctt tatttcatct tgagcccatt ttggaagttc ttttgccatt
    10441 ttatgtcacc agaatactag ttttgttttt ctttcttact tgattgccac cagtctctat
    10501 attcatcatg tttgtcctct ctagttctct cacgtcgatt taatctgtct ctaatgtcac
    10561 taatttgttc tcttgttgca tacaaaccac attttttaca gataaaatgt ttcgtgttaa
    10621 gatcaaattt catctctatg accacttctt tggctgcaca ctcggggcaa tcagtcaatg
    10681 acgccactca tcaaaattat ccataaaagc ctttggtcaa ttttctggta cgcggtctat
    10741 gtcgtcatcc ctttcggtca gttcgcccat cgaaaatgcc gcgtaccaga tctctcactt
    10801 gttaaagaaa aattataatt ctttctagtt gtcaagcatt tctgcaatta tcctagcagt
    10861 gttattggcc ccatttctgt caaaattttt ggtaaactct gtaagacgtt cttcatattt
    10921 gttgtagttg tttcgtatgt tctgaacagc gcttattact tgctttgtat tgtttgcaag
    10981 aactcctaat tttttgtctt ctgcccatct tatttgattt gtatgttcat catagactgg
    11041 aattcctatg attggttttg ctttcatacc aataatctca cccatagctg tgtgagagcc
    11101 gtttatgact gcgtatttac acaaacttaa caccgtattc ttctcttgct ctgataagaa
    11161 tccaacatca atctgaatcc agtctatctt ttgttcaagt gcatctgaaa tagagtaggt
    11221 tttaccgtct tttcctttta ccatatctaa cgaagcatca ttttgagcat gagaaacaag
    11281 tcttttctca tttttcaatt catctgaatg aaaagcctgt tcatatttcc tagccgtggc
    11341 ttctttagta gacttgttgc ctgtctgcat ccaatatcca aaagtttcag aattttcaat
    11401 taatttttct aaatccgatt ttggtttatc agttagtata tcagttgaga aatgacccgc
    11461 ataaaccact ttatgtttta tctcctctgg gaaatttaga ttatattcac atatggtatt
    11521 tggaggtgct gaatctgcta caactatctt tgtagccttg gcaatttgtt ttgagatgta
    11581 aataagcgat ggataaaagt aaaaatgaga tctccaaagt cttggcttaa actgatttgt
    11641 tacaaagaca gagtcaattt ttcttctttc agctaacaca tttgatccca tatcgccatc
    11701 atttatcaca agatcaaatt tttctttatt atacaaactg gcttcatttt ttagataact
    11761 agaaatttgt tttacaagtg gaggattacc agcaactgga agcaacaaat tcaacataga
    11821 aagtgtaaca cttggaccat gtttaccatc gattggagtt ggcatcaaaa tttcatggat
    11881 ttgtttcttt ttctgaggaa atttttgaag cagtctttga tatatctcac ccttgctaga
    11941 ataatgcatc tcgaattcat ctttgatgta actaggcaaa attgaatcca atgccatcat
    12001 tctgctataa tggccatttc cccaaggata gatgaattgt cctaacttta tcataatttc
    12061 agatcaattt gttcctgttt aaattctcaa tgattagatc ttaatgaatc aactatatcg
    12121 tgtatgtttt taggacctat tggaatagtg attattttct gtgaatgaag tgttgcctga
    12181 gaaattttgt aaatcttttc ttttggaatg cttttagtac taaattttaa tctacacaaa
    12241 ttctcaattt ttgagacata ttttcttaat ccgatttcat ttgcagtttc gaaaatactg
    12301 tcatctatac ctgaaagggg catccaagga atcttgcctt tttgaaatac acgtgtaata
    12361 ttatgttcaa caaaccacac gggaaagatt agtggtgata tggaaagcga aactctatct
    12421 attttggcct ttgtggcaac agataaaaga atttcagtta ctactgcaat tctaaacaac
    12481 agtccattat ttccagaaaa tttcatgtca agtttataaa aaccaagctc tattttggtt
    12541 tgagaaatta ttggcaaaat atgattcatc atctttactg tgttaagtaa ctccgattca
    12601 ttacggtaac atacaagtat ttttacatca aaacccatct tgataatctg aagacaagaa
    12661 attatcgaaa gctcatcata tagacaacaa agaatagtct ctttttgaga tttgtatggg
    12721 attccaccct ttcctttatc catgaaaata caaacatatg cataagattt tgttagatat
    12781 gtgtaaatca tcttgtcatg atttttttcg gttcctggct ttgcttgtaa atttactgtt
    12841 ttttctataa gtaatgatgt agcagcaagt tctacatcct ttggcatgta ggtagatgtt
    12901 ttaccatcca ctttgacata aaatttctct ccacttagaa caagattgac tccagcttta
    12961 gctatcgctg aaactacaga attaaagtta ttttctacct ctatagcaat tgcaaccttt
    13021 tctattccaa aaagcgagtt tattgtagtt gatgcaaaaa caggatcttt ggcttctaca
    13081 attatgaccg agtctttttt ttctatcttg ctaaagctct gtctttttgc tttaagagcc
    13141 gcagatatgc ttgaagctaa agagttgatc ttgttaagag aaaacaatga aggaaaaaca
    13201 acaactaccg tcttttcact cattttgttt cttagtttca agtcatttat aactctagga
    13261 tcgacatgtg tttgttctag caataattat atgaccagac ttgggacaat tttttatggg
    13321 taaatttact gccgaacaat tatctcaaat aaataacaca ctaaaaactc cacaagaaac
    13381 tctcaagtgg gctcttgata atcttcatcc caaacttgga tttgcatcta gttttggagc
    13441 agaagatgta gtcgttattg atatgttaat gaagataaat ccaaaagcta gaatattcac
    13501 acttgataca ggtaggctta atcaggaaac atatgatgtg atggatgaaa tacgaaacaa
    13561 atacaacatg aacattgagg tgatgtttcc agatcaaaat gaagtagaac aaatggtgaa
    13621 agtcaacggc atgaatctat tctatcacag tatggggaat agaaaacttt gttgtggaat
    13681 aaggaaagtt catccattaa ataaaatgct tgcaactttg gatggatgga taactggatt
    13741 aagaagtgac caaacacaga acagatcaga tgctaaaaga atcgaaatag atgaacagca
    13801 taataacatg gtgaaaataa atccaataat tgactggaaa tgggaacaag tttgggatta
    13861 tgtcaaacaa aataagatcc catacaacaa actgcatgac aagggatatc caagtattgg
    13921 atgtgaacct tgtacacgtg ctataaaacc aggcgaggat ctacgagcag gtcgctggtg
    13981 gtgggaaacc gacccagaaa aggaatgcgg tttacacgca gatcacggta acaagtgaga
    14041 ttttgacaga aagtaaaggt gtagccattc cacatggtgg caagctagta aacagattta
    14101 caacaaaaaa tacagacgat atgttttctg tttcagttga tactgatctt gccaatgata
    14161 tagaaaatat cgcagatggt atttttagtc ctcttgaagg atttttgtca cagcaagact
    14221 ttgagagtgt agtaaaaaat ggaaggctat ctaacaattt accgtggaca attccaatag
    14281 tatttgatgc agacaaacag actgcttcaa aaatgaaaga ttctcgcgat gttcttctaa
    14341 aaaactcaga aaacaatggt tttgcgattt tacatgtaga ggatgcatac agttttgata
    14401 agaatacaac cgcaaaagga atctatggta caactgatcc aaaacaccca ggtgtagcaa
    14461 aaacgatggc aatgcaggat tttcttatag gcggaaaaat agactatgtc aaaagaccaa
    14521 aagaaatacc tatacgaaaa tacaggaaaa caccaacaga aactagagca aattttgaaa
    14581 aagctgggtg gaaaacaata gttgcttttc aaacaagaaa tgtgccacac gtagcacatg
    14641 aaatgcttca gaaagcatca cttaacacac acgatggtct ttttgtaaat ccactcatag
    14701 gaaagaaaaa gtcaggtgat tttacagatg aagtgatagt aagtgcgtat gaaatattga
    14761 tcaaacacta ttatccagaa aacagatgtt ctcttgcaac acttcacacg gaaatgagat
    14821 atgctggccc aaaagaagca attcatcatg caataatgag aaaaaattat ggatgcacgc
    14881 atattataat aggtcgtgat catgcaggcg taggaaatta ctatactcca tttgctgctc
    14941 aagagatatt tgatgaatat caagatctcc agatcgagcc aatcttctat cctgcatttt
    15001 tctattgtaa aaaatgtctg tcgtttgcca gtgaaaagaa ttgtccacat ggaacagaat
    15061 ttcaagaaca gctaagtggt actaagctac gaacaatgat actggaaggc cagactcctt
    15121 cagaatacat gatccgaccg gaagtttcca aaatcattat gaattggaac aagccatttg
    15181 ttgactagat ttttattaaa gacgacggag ctaagtttgt gataaaaaat atttttctag
    15241 ttttaatgtt agttattaca atttttcctg tgtacgcgca aactgcctca aatccaactg
    15301 ttcgcctaga atctatcgat ataccttttg aaaaattcaa tgtaatcagc agtaattcaa
    15361 ttatgggtga atttgaacac gaacattcga taaactggca gctctctatt aaaaatcata
    15421 tagtatatgc aaacccactt ggtaatgctg taatacaatt gtatgatggt gtggataagg
    15481 aaaaattcat tgagattggg atgggtagtc cgccagacag aaaattctgg gtagcggtac
    15541 aaaatccaga gattggatac gcagtggttc acgatcaaac aaaggatggg tggaacccta
    15601 atgatgagat agttgtagta tactctagca ctgcggatct tacaataaat aatggccaaa
    15661 ggatagtggt ttccaacttg gatgttggta attttgaaat aaaggatttt tcagtttatg
    15721 ggatggaatc atcaactgat ccaccagcaa ctaattcagg taatttaata ctaaacatgg
    15781 tatctggaaa tcctgcagct aatccacttt tttatcttcc atacatactt gcgataggag
    15841 ctggagcact ggtagtagtt ctcttaaaga ctaagaagag aacctagtct tgtaatagtt
    15901 acactctttt ttaatttgac agacactaca cattggtgat atgggtttac aaatattttg
    15961 tccatacatt acaaaaacat cattaatttt aagccaatat ttttttggaa ctttttccat
    16021 taatgcaaat tctgtttcct caggtgtttt tgtttctaca agaccaagtc tatttgaaat
    16081 cctatgaaca tgcgtatcaa ctggtactgc aggtttatca aatgcatata caagtacgca
    16141 atttgctgtc tttctgccta cacctggaag ttttagtaat tcatctaaac tatctggaac
    16201 cttgccagaa tactgtgaat caataagtga tgcaacctca attattcttc ttgcctttac
    16261 atgataaaat ccagtagatt tgattattct ttcaacatca gagattttgg ctcttgcaag
    16321 caaacgagca gttttgtatt ttgcaaacaa ttttctgaca acagttgaag tattttcatc
    16381 tcttgttctt gcagaaagta tagttccaat tagaatgctg agtgggtctc cagattctgc
    16441 ctcacgcaat tctctaagcg cagtcattct gggtggtttg acagaattca tagtgtttat
    16501 cattccacgc agaatttttt ccatctgaac taattgcact ttttgtatta taaagaccat
    16561 acaagtatta tattcctaaa acagaataca gctgttggat ttaacaaagg atgaagaatc
    16621 tgccctaaag ggagagcaag gtgaaactct agctcttgca tacagaattc ttgtcgcaat
    16681 aggagaggct acaaatgcag atcgtctaat cccaatagaa tgggcacatc tttctggtgt
    16741 aaactataac accattggag attctggaga agaatttctt tcaaaattaa gtaaaaatgc
    16801 caaatttaag gtcaaaacca ctgtaaatcc aatgggtatt gattttgata atgtatcaaa
    16861 atatgatgta gatgaagaat ttattaacaa acaaaaaagc attaaaagtt catatgaaaa
    16921 aatgggagct atcacttcct tttcatgtat tccatatgaa atctttgatc taccaaagaa
    16981 aaacacacat gtttcatttg ctgaaagtaa tgcagcaatt tatgtaaatt ctgttagtga
    17041 tcttaaaaca aacaaagaaa gtgcttttag tgcacttgca agtgcattaa ctggtaaaag
    17101 tccatactct gatctacgaa aagacgaatc tagagatcca aaaattacaa tacaaatgaa
    17161 agttgatcaa aaagatgatc taatctatgg aatgcttgga tattttgcag gaaaagttgc
    17221 caacagctct gtagcatttt caggaataaa taaacttgac aagcgtagtt gtaaatccct
    17281 ctgtgctggt atgggtacgt caggaacttg tggaatgttt actttggatg gtaacaaagg
    17341 agaaaaagta gattttgata aaaaagaagc gcaaaaaatc catgatgaac taaacacctc
    17401 agaatctggt gatattattg cacttggaag tccacaactt ggtctagatg agttatcaga
    17461 tctggctggc atgctgaaag gtagatcatt caataaacaa tgcatgattt tttgccctag
    17521 agtagttcaa gaacaggcta gacgcattgg atatgcaaat gaaattgaac gtgcaggtgg
    17581 tgaacttctt tctgattgtt gtatttgcct cacaccgtta attaataaaa aaaatgtgga
    17641 tggagttgtt acaaacagcg taaagggtgc gtattatttg aaaacttcaa acggcgtaga
    17701 tgtaaatctt aaaagtctta atcaaatcgt acgcgaggaa acaaaatgac attaaaaata
    17761 attgttaaag gtaaagttga aggtgaggtc atggttgcaa agaacccgat caattttctt
    17821 ggcgcagtag ataaaaaaac aggaaaaata cgagatgaaa aacacgatct ttttgggaaa
    17881 tcactcgcag aaaaaattct tgtttttcct tttggagttg ggagtagtgt aggagcatac
    17941 acgatttact ctcttaaatt caacaaatgt gctcctcaag caatgatttg cttgaaggca
    18001 gatattacta ctgcatcagg ttgtgccctt gcaaacatac ccatggtcat agcaacaaga
    18061 gaagattatg actccttgat agatggaaag aaaattctgc ttgatgctga taatgggaaa
    18121 atactatagc aaaattcgta ccacatcccc gtcagggctg acttctttga aaacaattcc
    18181 ttcaaacttg agtattttcg tactcttgtt tttccaacaa tcttgtggtg ctccagcctt
    18241 ttcacatgtg tgattaagaa attcttcctc attccaacca tattctactg gcacttgagg
    18301 tagaagaaga ccagaaccaa attcccactt gacaattaag ccatctcgtc cggtttttat
    18361 cttacttggg tattctagtg gtttttcaac cttgatctca aggggtggag ttaatacggt
    18421 tacttcaaat gaaatgtctc ttagttcttc atactttact ttaggaaatc gtggatcttc
    18481 tgtagatgaa gctattgctg catctacaag cgattgatat agcttttttt ctggagtagg
    18541 aaagccaata catcctctaa gattttctgc tttattcaaa gtcacaaaga ctcctgaatt
    18601 gtaggaaaat ttagatttga attcctctgg caatatgatt ttttttccag tcttaagata
    18661 ttctgtaacc gcaagtctag cagtttttac tagagattca ccgtcttggt ctgagagact
    18721 cattgtaatc tgttgtttaa tttatcggca aattcttgaa gttttttaaa atgtgttcct
    18781 ttccagtaga tctttttgca actctcacac atccaaaact tctcttccct ttctagaatc
    18841 ccttctggaa ttttatttat tattctgtat ttctctattg cttgaagttt accattacaa
    18901 actgtacatc tggagttatc tgtgtctaca aaaatttttt ctagtcttat ctttgcatta
    18961 atttgtgcga tttgttccag ttcatcattt cctctgataa gtatagtcat aataccttgt
    19021 ttgtctgcac ttttagtgag ttgctcatct tttgtaacaa gcacccgttt ttcatttttt
    19081 gctatgatga caagtttctc atcctcgatg tcagaataaa atgtggaatc atatccaaga
    19141 atccttagtt tttttgctag gttgccaagc atagaatcaa ttacaaaaat aggtctcttt
    19201 tcactcattt gtttgcctcg ccttctccag attccactat gtgactttca ccagcaggta
    19261 aaactcgtat aaaatcatca atactgattg tttttacaat ctcctctttt tctttgaact
    19321 tgagaaattc tagttttatt ttctttgcaa tttcaaccat ttcagatccc gatggttcaa
    19381 ttattacata ataaatgctg ttttttttca ctggttctgg cggtccacac attaaagagt
    19441 atttttcatt tttttccaac agtcctacac atagacgcat gttagttact ttaacaaaat
    19501 ttctttgccc atcaattacg aaagaacctt ttgcaataaa ctggccgcta ggagctgcgg
    19561 tttttacctg atctgggtgt acccagtatg aatttaatcc ataaagtcct tctctccatg
    19621 ctctgctaaa acaaacagtt gcttgtgcag tttccattat acttgataca ttgatttcat
    19681 tactattttt taatatgaaa aatggtgatc caaaaatttc tgcatgaaac actttgtcgt
    19741 ctttttcaag atgttttctg attacagatg aattagatga tgcatcacga ccaccgatag
    19801 ccaaaagtcc atcggaagtg aaaaaccatc tgtatctttc ataccattct ctttttttct
    19861 gtactgtaaa tacaactgaa tcttctgcaa tgcttgcttg ttttctaaac ttatctaaac
    19921 tcttttctgt ttgttttttt gcagattcga ttaactcaat tgctctaagc tgacgttttg
    19981 attcattaaa taagactgat gctacagctg gtatcgaaga atcaggttct atcttgattt
    20041 tttcaccatc tatatcaata aacaaaatgc ccctttcttt cacaaatgaa gaatgcttat
    20101 tcacaagcat ttctaagatg gtaggactat caagcgtagt tattccactt gaaaccattt
    20161 ccataagtga tttagcaata ttggaaatct tgcttgcttt ttctttgact gctgatattg
    20221 cctttatctg ctcttctagt ttgtgttcaa actcttctat cttctgattt actgtatttg
    20281 cttgagttga tttaccatgt tcaagaagat tttctgtaaa aagtgaatct aatccttcca
    20341 tgaaactagt aattttagta cagtttgcat tttctgtttg tcctagagca atgggtaaaa
    20401 catcaacact tttttcggtt cgtactaaaa caggttcatg tatgccatta actatttttt
    20461 cagtaaggct tttaattgca gtgaaaatca tgtctatatc tttttcagaa agactgtttc
    20521 cagttgcttg tggatcaatt tttgcaagtc taattatctc ttcagcgtat ctggtaggaa
    20581 gaccaagtgt tctacctatc catcttacca ctggtgttgt aatcgataaa acctctgaaa
    20641 gttcttttga tgttatatta aaaatatcta atccactttg tggcggcggt tgatattcta
    20701 gacctaagtt taactctcta tgtcttacgt taattgagtg taagagtgca agaatcttca
    20761 tattcttatc acacaaaatt atatttccgt ctccaaaaaa ttctcctact agaacaaatt
    20821 cttgttcaag accagaaaat gttatgtatg caattctttc tgcgccaatt tgttttattt
    20881 cagaaattct tagtctgaga agatcatttc ttagtcgttt tactagtcga ttttcttcaa
    20941 tctggtctat cttaactgta gtaatccaag tgcctaatgt tgaaaacatt aacaaaatat
    21001 ctggtttctc aggatgatgc agtttgaaaa gcaggctgtc gcgacttatt ccataaatgt
    21061 tacttacata gtactcctct gtttttttac taatttcatt taccaaataa cgtagttcta
    21121 tcccggctag tgtcatgata acgcattatc attccaaaat tatactgttg ctattgaaaa
    21181 cttgtgaacc aaagatttta aacaagggtt atcgacaatc cttttgtaaa tttacttgac
    21241 taggcatccc aagaaaaaag aaaacgaaaa aaaatcggaa tcaggaaatc ctatagataa
    21301 ccctcctcag tctgagccta acacaaacca gactgaagga ataaagatga aggatcctct
    21361 caacaaactg ttctggattc gtgtagctct tggaattcta gcaggaacca tttcagcaca
    21421 agttgggagt tctgtggaca tgtcatcaag agcatttgtg ggatttggaa tcatgattat
    21481 tttgtttatt gtgtcatacg gaatagcaaa agcaatgcac cttccacttc caccctctga
    21541 cagaaaaaaa ctcataatga caggaatagg aagctacttt ttcatgttca tattcacatg
    21601 gattctagtt aataccctgc tacatcctgc tgcagtaaca agcttgaatc ctatcaagta
    21661 gcactaaaat atctaatcgt atcattgtgt tagatgtgga aatatgaaac cccaggaatt
    21721 ccagatgagt tttttgagag agtagaggaa gttcctataa caaaagaaga ggtacgagct
    21781 gttcagataa gcaaagcaag actctctaca ggccaaactg tatttgatat tggttgtgga
    21841 agtggctcta tatctgttga agctgcatta caagttaaat cgtctggaaa agtgtattct
    21901 gttgattttg atcccaaagc aattgaatta actaaaaaaa atcttgccaa atttcaaatt
    21961 acaaatgtca tagtaacttt gggaaatgcg atagacaaaa tttcagaatt gcccatggct
    22021 gacgcaattt tcatcggtgg aactggtggt gatacaaaag acatactcaa gctctgcgaa
    22081 ggcaagctaa aatcgggagg caggatagtt attggaacaa tcttagtaga aactctagtt
    22141 gctgttttgg aggtaataga taagctaaag ttttcctcag tagatgtaac tcagatcacc
    22201 gtttcaaaga gtcgtaaaac ttccactgga acaatgatgc tcgcaagaaa tccagttacc
    22261 ataatatctg caactaaaat ctaactttta attaggctca acttgaatcg catttgatgg
    22321 ctgagcttac atgtgttggt tgtggtccag gtgatcctga ccttttaact gtaaaagctg
    22381 taaaggcaat tcgtaatgct gatgttataa tgtgccctac ttcaaaagaa ggaaaaacca
    22441 gcattgcata ttctattatt tcttcagtaa tagatggatc aaaaaaacca gagatcataa
    22501 atttggtttt tccaatggtg aaagacaggg aaacactaga ggatacttgg gaaaaaaatg
    22561 ctaaggcaat agcatctgcc ataaagtctg gcaaaaatgt agtttatctt actgtaggcg
    22621 atccatcgct ttatagcact tggatttatc ttcagcgcga acttaaatca aaatatccag
    22681 aaattaagct caccgtaatt cccggaatag tatccatgtt tgcttttgca tccaaagttg
    22741 gtattagtat tgcagaaggt gatgaaactg ttgcagtaat tcccgcttgt tatgaccttt
    22801 caagagtaaa agaaacagca aaaaattgtg atacaatgat atttctaaaa gatggtagat
    22861 actttgatca agttattacg cttttaaaag aagctggatt tccagataat tctatttttg
    22921 caatagcaca ggatgttggt actgaagaag aaattgtaaa aaaattaaca cttggtgaag
    22981 tcacaaaaga tactttgaca agcaagtatt tttcaataat ggtggtaaaa cgtgcataag
    23041 gtctattttg ttggttgtgg accaggtgat cctgaacttc ttactgtcaa agcaaaaaaa
    23101 ttgattcaga aagctgacgt tgtagtttac tcaggatcat taattcctac acaaattatc
    23161 aatttatgta aaaaagaaaa atacgatgct gcaaaactcg taagggaaga aatttttgat
    23221 attctaaaaa ctaatacaaa gaaaggaaaa ttggcagtta gattgcatga tggagatcct
    23281 agcatttatg gcgctatacg tgaacaaaca gataatctta gaaaagctgg aattgaattt
    23341 gagattgtac ctggagttac ttcattttta gcatccgcag cggttttggg ttctgaactc
    23401 acattgcctg gtattactca gacgattata attacaaggg cagaagcacg aactaaagtt
    23461 ccaaaacgag agaaaatttc cgaacttgca aaacacaaat ccactatgat cttttatctc
    23521 agtgctcatc tcctctcaga tatcgtaaag gaagtaatct ctggaggcta tccaaaatcc
    23581 accccagctg ctgcagtata tcgtgcaagc tggaatgatg agaagatcat agttggaaca
    23641 ctaaaagaca tcacaaaaaa agtaagagat gcaaaaatca ctcgtactgc aattataatt
    23701 attggtgata ttgtaaaacc aaaatcttat gaatattcga agctatatga taaaaccttt
    23761 agtcatggtt ttagaaaagc aaaaattaaa tcataatttc attacttggt ttttaattcc
    23821 aat
//
LOCUS       DATIDL010000103         3878 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrosopumilaceae archaeon isolate SMAG_U16342
            k141_934305, whole genome shotgun sequence.
ACCESSION   DATIDL010000103 DATIDL010000000
VERSION     DATIDL010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935836
            Sequence Read Archive: SRR25158326
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosopumilaceae archaeon (soil metagenome)
  ORGANISM  Nitrosopumilaceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrosopumilales;
            Nitrosopumilaceae.
REFERENCE   1  (bases 1 to 3878)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3878)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/04/2024 05:09:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,520
            CDSs (total)                 :: 1,474
            Genes (coding)               :: 1,474
            CDSs (with protein)          :: 1,474
            Genes (RNA)                  :: 46
            rRNAs                        :: 1 (5S)
            complete rRNAs               :: 1 (5S)
            tRNAs                        :: 43
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3878
                     /organism="Nitrosopumilaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_934305"
                     /isolate="SMAG_U16342"
                     /isolation_source="soil"
                     /db_xref="taxon:2202732"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..352
                     /locus_tag="VLB45_04655"
     CDS             <1..352
                     /locus_tag="VLB45_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007551554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HSD05027.1"
                     /translation="CVFEKILTRSNKTSAIKTSEIRREISQKSKVNESEIFIDASKTP
                     SIPLTPSKKESESIILTSKKDLLPEAYELPISEIPLISAISGFMNMLRVYTAEKNRKK
                     VEIAAKIILGENKS"
     gene            349..1026
                     /gene="pyrH"
                     /locus_tag="VLB45_04660"
     CDS             349..1026
                     /gene="pyrH"
                     /locus_tag="VLB45_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HSD05028.1"
                     /translation="MKKRIVIKLSGKLFGFEDSKILKDYASFLVRISKFCQPVIVAGG
                     GKIARHYISHARSSGADESTLDELGIEVSRLNAKLLIYALQNKAYPHPPTNLKEVSHA
                     ADSGLIVVSGGLHPGQSTNATAALIAEKVHATVFLNATDVDAIYDSDPNKNKNAKKFR
                     QIEIKKLRSMLVHQESIAGGYDLMDIVALKVIERSKIKTRILKADIKTLEKAIKGSPE
                     GTEIILT"
     gene            complement(1013..1327)
                     /locus_tag="VLB45_04665"
     CDS             complement(1013..1327)
                     /locus_tag="VLB45_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016939423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05029.1"
                     /translation="MQYHQAVRIGQERARKSQMNLFSHAGFAMLTLTVKESDGKFVPV
                     GEQELVAVVKKPEGWVTVIVDEEGFAKAQSKALEETEAREILKKAVAAGIPEYSGKVR
                     LV"
     gene            1406..1963
                     /locus_tag="VLB45_04670"
     CDS             1406..1963
                     /locus_tag="VLB45_04670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05030.1"
                     /translation="MNRKLLVGIAVGILGIIIVAMAVLPGSGILKKILPDNVSLPGSL
                     DTLSSDIIPLAVKLNKVTVESVSEKEAVLELEFDVSNPNDATVLLEMISFEIFANGEK
                     VGFGEIGQRLSGQYTSSNYYTVLSDYPLTITGKATIKNTGKNPEFWSTINDSIPEWRI
                     TGEAFYSTTSAFSGIAGSTVFDYTK"
     gene            complement(2212..2859)
                     /locus_tag="VLB45_04675"
     CDS             complement(2212..2859)
                     /locus_tag="VLB45_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05031.1"
                     /translation="MGDRDYRRIITNVIEGISRDIKQQIDIKDNKTIHFYEIVRCLRR
                     SYFDRIAPKEEKTIGFTNIMSDLLRKAAYGSTNGEFALDSIKLKIKADMVVDDVVIIF
                     QMVAKLPENPLPSDALYLNACLWILNKDEGIIVYLTGDGKEASFSMGKDKKMFEESIR
                     RVRVLSDLLEEKKTPIIEPSPECNTCQYYEECYIKKKKSASISLENLLGLKDQDK"
     gene            complement(2897..3196)
                     /locus_tag="VLB45_04680"
     CDS             complement(2897..3196)
                     /locus_tag="VLB45_04680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05032.1"
                     /translation="MESSKQKNSDSIDEVRRAKINLKRMFTKRQELTVETVVNTVWIS
                     TFLALILTIPSLIIFMGIYFSTGNILLGAVVGFGLHFIGLAFSAKISEGLLRIMS"
     gene            complement(3447..>3878)
                     /locus_tag="VLB45_04685"
     CDS             complement(3447..>3878)
                     /locus_tag="VLB45_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HSD05033.1"
                     /translation="IGMDVDFYNLLSILRGKFWGLDEQQIQDLIVTHTTSAPKDLLAR
                     MISAESVKDALGELSTTIYKDLIPQTDNAIDALSEFERGFELATYRAINRTFTRMFSF
                     ATVIGVLKLTTYEIRNIAAIAFAVEQQIPVQTVMSKLVIAE"
BASE COUNT         1273 a          727 c          657 g         1221 t
ORIGIN      
        1 gtgtgttttt gaaaaaattc ttactcgaag caacaaaact agcgcaatta aaacatctga
       61 aataagaaga gaaatttcac aaaaatccaa agtaaacgaa tcagaaattt tcatagatgc
      121 atccaaaact ccctccatac cgcttactcc atcaaaaaaa gaatcagagt caatcatatt
      181 gacttcaaaa aaagatttac ttccagaggc atatgagcta ccaatttcag aaattcctct
      241 catctctgca atctccgggt tcatgaatat gctacgagtt tatactgcag aaaaaaatcg
      301 aaaaaaggtt gaaattgcag cgaagatcat ccttggtgaa aacaaatcat gaaaaaaaga
      361 attgtaatca aactatcagg aaagctattt gggtttgaag attccaagat tctaaaggat
      421 tacgcatcat ttcttgttag aataagcaag ttttgtcaac cagtaatagt agcaggagga
      481 ggtaaaattg cacgacatta catttcacac gccagatcat ctggtgcaga tgaatctaca
      541 ttagatgaac ttggaattga agtgtccaga ttaaatgcaa aacttttgat ttatgcacta
      601 caaaacaagg cttatcctca cccgccaact aacctaaaag aagtttcaca cgctgcagat
      661 agtggcttga ttgtggtctc tggtggtctg catcctggcc aaagtacaaa cgcaactgcc
      721 gcgcttatcg cagaaaaagt tcacgctaca gtttttttga atgcaactga tgttgacgca
      781 atatatgatt cggatccaaa caaaaacaag aatgcaaaaa aattcaggca aatagagata
      841 aaaaaactgc gtagtatgtt agtgcaccaa gaatcaattg ctggaggtta tgatctgatg
      901 gatattgttg cactaaaagt aattgaacga tctaaaatta aaacacggat actaaaagca
      961 gacatcaaaa cactagaaaa agcgataaaa ggttcccccg aagggaccga gattatacta
     1021 acctgacttt tcctgaatat tctggaattc cagcagcaac agctttcttt aaaatctctc
     1081 gtgcctcagt ttcttctaaa gctttggact gtgctttggc aaatccctcc tcatctacta
     1141 tcaccgtaac ccaaccttca ggtttcttaa ctactgcaac taactcttgt tccccaaccg
     1201 gtacaaattt tccatctgat tcttttactg taagtgttaa catggcaaat cctgcatgtg
     1261 agaaaagatt catctgagat tttcttgcac gttcctgtcc aattcttact gcttgatgat
     1321 attgcacaga atcaatttgc aaaaactttg actataagcg ttttcagctt aacatttaag
     1381 agaagtacaa atctccctat aatgattgaa cagaaaatta ttagttggaa tagcagttgg
     1441 aatattagga ataattatag tggctatggc agtattgcca ggttctggca ttttaaaaaa
     1501 aatactacct gacaatgttt cacttcctgg cagtcttgac acactttcat cagacataat
     1561 tccacttgct gtcaagttaa acaaagtaac cgttgaatca gtttccgaaa aagaagctgt
     1621 tttggaatta gagtttgatg tttcaaatcc aaatgacgca acagttttgc ttgaaatgat
     1681 aagctttgaa atctttgcaa atggagaaaa ggtaggtttt ggagaaattg gacaaagact
     1741 ttctggtcaa tacactagtt ccaactatta cacagtactg tctgattatc ctcttactat
     1801 aaccggcaaa gctacaataa aaaatactgg taagaaccca gaattttggt ctacaataaa
     1861 tgacagtatt ccagaatggc gaatcaccgg agaagcattc tatagcacaa cctctgcctt
     1921 tagcggaatt gcgggttcta cagtttttga ttataccaaa tgatcaaata ttgatagtat
     1981 atcataaaat ctaaaaattt tgattatttg tacaaagcaa aacttgttta gataaattat
     2041 ataaggattc aaaaagaggt ttttgtcatg gcagaagcct caataggagt atttggtgca
     2101 actgtcggtg tctttatcgc ccttgctgta gtcacatttg cattacgtgg caaaggacat
     2161 aagatatccg atgtggtatc agaataaagg gaacaaatcc cctgtacttt tttacttgtc
     2221 ttgatctttc aaccctagta gattctctag agaaatactg gctgattttt tctttttgat
     2281 ataacattct tcataatatt gacaggtatt gcattctggt gatggttcta ttatgggcgt
     2341 ctttttttcc tcaagcaaat cgcttagaac tctaactcta cgaatagatt cctcaaacat
     2401 cttcttgtcc ttgcccatag aaaaagacgc ttccttaccg tctcctgtca gatacactat
     2461 gatgccctcg tccttattta aaatccataa acatgcatta agatacaatg catctgatgg
     2521 gagtggattc tctggtagtt tggctaccat ctgaaatatg ataactacat catctactac
     2581 catgtctgcc ttgatcttta gttttatact gtcaagtgca aattccccat ttgtagaacc
     2641 ataagcagct tttcgtaaaa gatcactcat gatattggta aaccctattg ttttctcttc
     2701 ttttggtgca attctatcaa aatatgagcg gcgcaagcac ctaacaattt cataaaagtg
     2761 aatcgtcttg ttgtctttga tatcgatctg ctgttttatg tcccggctaa tgccttctat
     2821 tacatttgta ataatccgcc tgtagtctct atcgcccatc aaggtaggta tccttgatgt
     2881 cttgtctata atagatttag ctcattatgc gtaaaaggcc ttcagatatc tttgcagaaa
     2941 aggcaaggcc tataaaatga agaccaaaac cgacaactgc tcctagcaga atattgccag
     3001 ttgaaaaata gatccccatg aatatgatta gggaaggaat cgtaagtatc aaagcaagaa
     3061 atgtgctaat ccaaactgtg ttgactactg tttcaacggt taactcctgt ctttttgtga
     3121 acattctttt caggttaatc tttgcacgtc gcacctcatc tatagaatct gaattttttt
     3181 gcttagatga ttccatttga tgtaatatct atacaaatta ggttataaat ttacatgata
     3241 aaattatttt tttaaattaa ttatatagta acaatcctat atcagtagga taaaatcata
     3301 gaattattac aaaattcatc attataatga ataatttaca aaagataaaa cgatttttgg
     3361 tctctgagca agttaaaaat ctctataaaa attctagaaa acaagagaaa tcataggttt
     3421 ttgaacaaaa tagcagattc tgcagtttat tctgctatga caagttttga cattactgtt
     3481 tgaaccggaa tttgctgctc tactgcaaat gcaattgcgg cgatgtttct gatttcatat
     3541 gttgttaact ttaaaactcc aatcaccgtc gcaaagctaa acatccttgt aaaagttcgg
     3601 ttaattgctc tataggtggc aagctcaaaa cctctttcaa attctgagag cgcatcaatt
     3661 gcattgtctg tttgtggtat gagatctttg taaattgtag ttgaaagttc tccaagtgcg
     3721 tctttaactg attctgcact gatcattctt gcaagcaaat cttttggtgc tgatgtagta
     3781 tgtgtcacta tgagatcttg gatttgttgt tcatccaatc cccagaattt gccacgcaaa
     3841 atgctaagca aattgtaaaa gtcaacatcc attccaat
//
LOCUS       DATIDL010000104         3474 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrosopumilaceae archaeon isolate SMAG_U16342
            k141_2960452, whole genome shotgun sequence.
ACCESSION   DATIDL010000104 DATIDL010000000
VERSION     DATIDL010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935836
            Sequence Read Archive: SRR25158326
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosopumilaceae archaeon (soil metagenome)
  ORGANISM  Nitrosopumilaceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrosopumilales;
            Nitrosopumilaceae.
REFERENCE   1  (bases 1 to 3474)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3474)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/04/2024 05:09:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,520
            CDSs (total)                 :: 1,474
            Genes (coding)               :: 1,474
            CDSs (with protein)          :: 1,474
            Genes (RNA)                  :: 46
            rRNAs                        :: 1 (5S)
            complete rRNAs               :: 1 (5S)
            tRNAs                        :: 43
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3474
                     /organism="Nitrosopumilaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2960452"
                     /isolate="SMAG_U16342"
                     /isolation_source="soil"
                     /db_xref="taxon:2202732"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..375)
                     /locus_tag="VLB45_04690"
     CDS             complement(<1..375)
                     /locus_tag="VLB45_04690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05034.1"
                     /translation="MLVHFIFVMKKEDLVARTWEFEYISQMAQFYKHWINKTFSKQVD
                     VQADQMPVKPSWFNRINIGSLLNDHRKRGEDVYHFYLTYFRPLWTDCTCEGYHAQNFG
                     MVWWQPSPNKDDLLFHAEKNCTT"
     gene            428..1282
                     /locus_tag="VLB45_04695"
     CDS             428..1282
                     /locus_tag="VLB45_04695"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HSD05035.1"
                     /translation="MPKFDPESYKKRIVNIWNEVALKYHTDWASKNTGPFKSTSILVK
                     TSKIKKGDRVLDLACGTGAVTKKISSRIGPSGKVVGIDISSGPVKIAKKWNVRKNVKF
                     VIADVEKMRFSEEFDSVTCQYGLMFFPNVQIALRNTRRVLKNGGRITVAVHGSKKTAP
                     YFSCISNAILKFIPDFIPPGSPTVHRFGTKNLLKKEFVNAGFKKIKIHELNFEYKPGT
                     FEDYWSDYITNLSTPLKEKLKSLDANQFIIMKKMMKKNTERFTKKGKIVFPWKVLILT
                     AINPKKVN"
     gene            complement(1283..1522)
                     /locus_tag="VLB45_04700"
     CDS             complement(1283..1522)
                     /locus_tag="VLB45_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HSD05036.1"
                     /translation="MATAYVLINCELGSEETIIGQLKSIEGVKEVHGTFGAYDILAKI
                     ESPSVEALREVITWKIRKIEKIRSTLTLMGIEGQS"
     gene            complement(1579..1989)
                     /locus_tag="VLB45_04705"
     CDS             complement(1579..1989)
                     /locus_tag="VLB45_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010191338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HSD05037.1"
                     /translation="MINYWLVKQEPFTYNYAALEKEKKTTWDGVHNNLALKHINSMKK
                     GDQAFFYHTGDEKQIVGILEIISNPYANPKEDNKRFVVVDVKAVKSLKKPVTLDEIKK
                     DSMFKNWELLRISRLSVMPVSKKLWDEIIKKSNH"
     gene            complement(2202..>3474)
                     /gene="pheT"
                     /locus_tag="VLB45_04710"
     CDS             complement(2202..>3474)
                     /gene="pheT"
                     /locus_tag="VLB45_04710"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HSD05038.1"
                     /translation="LHNGLGRRRKKASIGIHDLDKISFPLRYSTVGKNHKFVPLASSS
                     EMTISEILEKTEIGIKYGQILGNSSKVPVILDSTGKTISFPPIINASLTTVSQKTRNL
                     LVEVTAMEKNTAEDTLAVVANTLQDAGFALFSTKISGANNSTPSFVPRNIILETDLVN
                     KILGLDLSATTIVASLRKSRLDATVKGKKILCTIPRYRTDIFGTMDLVEEVALGYGIE
                     NLEPTIPESLFAGHKNKVTTALDSVSSVMVGLGYFEAMNFGLTSKQVQYDLTKRDPSK
                     IISVADSKSQGYTILRDAILPGLIDTLSRNIHETYPQRLFESGIVFSKASDGVKEETH
                     LACICAHNDVSYTEIKSVLQSAFKTGFGMSCDTQMTSNPMFSEGRAANIVIGNKTVGI
                     IGEISPEIIDNFKLRVPVAGFEVKLTGLIFD"
BASE COUNT         1048 a          615 c          635 g         1176 t
ORIGIN      
        1 tgtggtacag tttttttctg catgaaatag cagatcatct ttgtttgggc ttggttgcca
       61 ccaaaccata ccaaagtttt gtgcatgata tccttcacag gtacaatcag tccagagcgg
      121 tctaaaataa gtcaggtaga aatggtaaac atcttcacct ctttttctat gatcatttag
      181 gagagaccca atgtttattc tgttaaacca tgagggtttt actggcattt gatctgcttg
      241 cacatctact tgttttgaga aagttttgtt aatccaatgt ttgtaaaatt gcgccatttg
      301 tgatatatat tcaaattccc acgttcttgc cacaagatct tcttttttca taacaaaaat
      361 aaaatgtaca agcatagttt gcaagaatcg aagttagagt taaaagctct tcgctaatca
      421 aaaagttatg ccaaaatttg atcctgaatc atacaagaaa agaattgtaa acatatggaa
      481 tgaagttgca ttaaaatatc atacagattg ggccagcaaa aacactggtc cgtttaaaag
      541 tacgtcaatt ttagttaaga cttcaaaaat caaaaaagga gacagagtat tggatttagc
      601 ttgcggcaca ggtgcagtga caaaaaaaat ttcttctaga attggtccta gtggaaaagt
      661 tgttggcatt gatatttcgt ctggtcctgt aaagatagca aaaaaatgga atgttagaaa
      721 aaatgtaaaa tttgtcattg cagatgtaga aaagatgcgt tttagtgaag agtttgattc
      781 tgttacgtgt cagtatggat tgatgttttt tccaaatgtt cagattgcgc taagaaacac
      841 ccgtagagtt ttgaaaaacg gaggcaggat aacagttgca gtacatggaa gtaaaaagac
      901 agcaccgtat tttagctgca tatcaaacgc aatactaaaa ttcataccag attttattcc
      961 ccctggatct ccaacagtac atagatttgg aacaaagaat cttttgaaaa aggaatttgt
     1021 aaatgccgga ttcaaaaaga tcaaaataca tgaattgaat tttgagtaca aacctggaac
     1081 atttgaggat tattggagtg attatatcac aaacctatca actccactaa aagagaaact
     1141 caagtctctt gatgctaatc aattcataat aatgaaaaaa atgatgaaaa agaacactga
     1201 aaggtttaca aaaaaaggaa aaattgtttt tccgtggaag gttttgattc ttactgcgat
     1261 taatccaaaa aaagttaatt gattaactct gaccttctat tcccatcaga gttagtgtag
     1321 aacggatttt ttcgatttta cgaattttcc aagtaattac ttctcgtaac gcttctactg
     1381 atggcgattc gatcttggcc aagatatcat atgcgccaaa tgttccgtgc acttctttga
     1441 caccctcgat agactttagt tgcccaatga tggtttcttc cgaaccaagt tcgcagttta
     1501 ttaatacata tgctgttgcc atagcatcct aatacaatgc aaatatatca atagatctat
     1561 cgtatagcaa tttttctatt aatgatttga tttctttatg atctcgtccc agagtttttt
     1621 cgatactggc atcacagaga gccgagagat gcgtaaaagt tcccaatttt taaacatgct
     1681 atcttttttt atctcatcca aagtcactgg ctttttgaga gactttactg cctttacatc
     1741 tacaacgaca aatctcttgt tatcttcttt tggattagca tatggatttg atataatctc
     1801 aagtattcca acaatttgtt tttcgtctcc agtatgatag aaaaatgctt gatcaccttt
     1861 tttcatcgag ttgatgtgtt tgagagctaa attgttgtgt actccatccc atgttgtctt
     1921 tttttctttt tcaagtgcag catagttgta tgtgaacggt tcttgtttga ctaaccaata
     1981 gtttatcatt tgataatttt gtaattaatg attttatgtg cttttttgtt tagtaggttt
     2041 gttccccggt tctgacccac aaaaaagtca ttggtagcca tcagcgtacc ctcgttcttg
     2101 ttcttttacc ggggtgccct gtcgcacggc tgggtggacc aaaaatcatg ttttatcatc
     2161 ccagtccgtc tgcgtgcatt tatgggcaca ttgatttact atcagtcaaa tattaatcca
     2221 gttagtttta cttcaaatcc agctactggg actctaagct tgaaattatc aataatttca
     2281 ggagaaattt ctccaattat gccaacagtt ttgttgccga ttacaatatt tgcagcccta
     2341 ccttcagaaa acattgggtt tgatgtcatt tgcgtgtcac aagacatacc aaatcctgtt
     2401 ttgaatgcag actgtaaaac agattttatc tcagtatagc tgacatcatt atgcgcacag
     2461 atgcatgcaa gatgcgtttc ttcttttaca ccatcagatg cttttgagaa gactatacca
     2521 ctctcaaaga gtctttgcgg gtatgtctca tggatgtttc tagaaagtgt atcaatcagt
     2581 cctggcagta tagcatcacg gaggatggtg tacccttgac ttttagagtc tgccacagag
     2641 attatcttag atgggtctct ttttgtcagg tcatattgta cttgtttact tgtcagacca
     2701 aaattcattg cttcgaaata tccaagacca accatcacag aactaacgga gtccagtgca
     2761 gtggtaactt tatttttatg tcctgcaaaa agagattctg ggatcgtagg ttctagattt
     2821 tctattccat atccaagagc aacttcttct accagatcca tcgtaccaaa aatatctgtt
     2881 ctatatcttg ggattgtgca aagtattttt tttcctttaa cagttgcatc caatctgctt
     2941 tttctaagtg atgcaactat tgtggtggca gagagatcaa gaccaagaat tttatttact
     3001 agatcagttt ctaaaatgat gtttcttggt acaaatgatg gcgtagaatt gtttgcacca
     3061 gaaatttttg tcgaaaataa tgcaaatcct gcatcttgaa gagtatttgc tacaactgct
     3121 agtgtgtcct ctgcagtatt tttttccata gctgtgacct caactaagag atttcgtgtt
     3181 ttttgcgata cagtggtaag agatgcatta attattggtg gaaatgaaat tgttttacca
     3241 gtagaatcca agatgacagg cactttggat gaattaccaa gaatctggcc atacttaatg
     3301 ccaatttctg ttttttctag gatttcagaa attgtcatct cagaagatga tgccagtgga
     3361 acaaacttgt gattcttgcc tactgtggaa tatctgagag gaaatgagat tttgtcaaga
     3421 tcatggattc caatagatgc tttttttcgt cgccttccca atccgttatg caga
//
LOCUS       DATIDL010000105         5781 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrosopumilaceae archaeon isolate SMAG_U16342
            k141_1343480, whole genome shotgun sequence.
ACCESSION   DATIDL010000105 DATIDL010000000
VERSION     DATIDL010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935836
            Sequence Read Archive: SRR25158326
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosopumilaceae archaeon (soil metagenome)
  ORGANISM  Nitrosopumilaceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrosopumilales;
            Nitrosopumilaceae.
REFERENCE   1  (bases 1 to 5781)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5781)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/04/2024 05:09:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,520
            CDSs (total)                 :: 1,474
            Genes (coding)               :: 1,474
            CDSs (with protein)          :: 1,474
            Genes (RNA)                  :: 46
            rRNAs                        :: 1 (5S)
            complete rRNAs               :: 1 (5S)
            tRNAs                        :: 43
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5781
                     /organism="Nitrosopumilaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1343480"
                     /isolate="SMAG_U16342"
                     /isolation_source="soil"
                     /db_xref="taxon:2202732"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..543
                     /locus_tag="VLB45_04715"
     CDS             <1..543
                     /locus_tag="VLB45_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008297889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HSD05039.1"
                     /translation="FINALKNSLEGGQENKPKGMLLTATLTEIKGGTSHNVTAIECDA
                     IMDIRIPVDMNCKMVGEKIATTVQEISKKQGVSAFYSVLDETEPFEAPHTSPLVRALT
                     LGVLDVEKNRPQLIRKTGTGDMNIIGNALNIPVVTYGPGDPHASHTIDERVSVDEYLR
                     SIEVFKHMLYHLKRLHVKSK"
     gene            591..1637
                     /gene="dph5"
                     /locus_tag="VLB45_04720"
     CDS             591..1637
                     /gene="dph5"
                     /locus_tag="VLB45_04720"
                     /EC_number="2.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004164 - diphthine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0017183 - protein histidyl modification to
                     diphthamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphthine synthase"
                     /protein_id="HSD05040.1"
                     /translation="MLWFAGLGISGTDGMGLDVVKILKQADIVYLEIFTSPITKPELA
                     KIKKLVKGKFKTAPRWLVEDGKAILEDAKRKEVVLLSYGDPYIATTHIELRTRAISEK
                     IKTGTIHAASAITSLVGECGLHYYKIGKIVTMMQEQQSASSVYYTTYDNLVAGNHTII
                     VLEYNQDAKFFLNPKDALNNLLLTESGQKRNVVDESSFAIVASRIGSKTQKIVAGKFS
                     SLKKTDFGKPPHSIIIPGRMHFTESDALKVLARCLDEPFDNSSRIKKISDQMLEKYIP
                     KARKALEDVTNRFKDDKGIMSVLENAELYLDDAEKFQSQGNEELAVLSIGYAEGLIDA
                     LRFSKGIDPWAQSL"
     gene            1676..2293
                     /locus_tag="VLB45_04725"
     CDS             1676..2293
                     /locus_tag="VLB45_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HSD05041.1"
                     /translation="MDALDKEIISALYVSSLSDEDPISIIRRKLPVNPDHLKAKIDFL
                     AKNGFVEEDKKTLTDIGRSSIRVVLAGGVFDIIHPGHLHTLRGAKALGNVLIVVIATD
                     QTAQKMKSRIPLHNMELRRDLVNSLSMVDYAVVGHEGDIFKTVARVKPDIIALGYDQV
                     HQEKFITDGCKKINLSVSIARLQSPIPDIKSSKIESEYGESIYRI"
     gene            complement(2290..2460)
                     /locus_tag="VLB45_04730"
     CDS             complement(2290..2460)
                     /locus_tag="VLB45_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007550853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05042.1"
                     /translation="MVLIWIGIAVGTIITVALIYKIIKSSKRSFVSLKCTKCGFKTNG
                     LKCPICENEKRY"
     gene            complement(2505..3191)
                     /locus_tag="VLB45_04735"
     CDS             complement(2505..3191)
                     /locus_tag="VLB45_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010193329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF120 domain-containing protein"
                     /protein_id="HSD05043.1"
                     /translation="MSELKVQHILTLAELLSKGARHNFVSITTTSLGKSINRSQQAAS
                     KHLLELERDGYIERIRNGQKVSVRITSKGQAEITKISSILKSSLESSPSYIEFKGTII
                     SGMGEGAYYMSMKGYTKQFKSKLGYVPFPGTLNVKLNDKKFTEAKRELDTHQGIMING
                     FSDGKRTYGWVKCYPAKINNSVDGMMITLERTHYDDSIIELISQTSIKKAAKLSTGSQ
                     ISIRVPMHSK"
     gene            complement(3199..4344)
                     /gene="dnaG"
                     /locus_tag="VLB45_04740"
     CDS             complement(3199..4344)
                     /gene="dnaG"
                     /locus_tag="VLB45_04740"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase DnaG"
                     /protein_id="HSD05044.1"
                     /translation="MPSTGIVKYHVKLSFDVDGLVEKADIIGAIFGQTEGLLGPEMNL
                     NELQKVSKVGRIEVNTTATSNQTKGDALMPMSTDITTAALIAAAVESIDKVGPFQAHF
                     KLEAIDDVRSAKKKEIVDRAKEIVQKWSTKTISEGEEMLKDVSEGGSSGKLATFGREK
                     LACGSGVFDSPWIILVEGRADVINLLRAGFDNALAIEGARIDESIKELCESKSKVVAF
                     LDGDRAGGFILKELKAMVHVDVVHRAPEGVEVEELTPQQIADILKETAEDMKKETAKP
                     RLHDPKDEAISNVAKKVYENLNESLEAVALDDGLNELFKVPVSEVVGKITSGSGIKYL
                     VLDGIITQRLIDAAKQAGVEYVIGHRMANLKNTDGLILKTFTELGVA"
     gene            complement(4402..4791)
                     /locus_tag="VLB45_04745"
     CDS             complement(4402..4791)
                     /locus_tag="VLB45_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HSD05045.1"
                     /translation="MKITEDEINALQDFIASLNSKDSVVVVEGKRDSTALKKLGYRGT
                     ICEFHSFKGLTRFADSMDSYKKLVLLLDLDRKGRYLTSRIIKLLEHRTKIDLSFKKKL
                     VAITKGKIRHIEDLSRYELPIRSIPSS"
     gene            4844..5533
                     /locus_tag="VLB45_04750"
     CDS             4844..5533
                     /locus_tag="VLB45_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HSD05046.1"
                     /translation="MSDILVLQNAKYEGLAGLGRLLAEDGFKVKTVIAKKEKIPFPDS
                     SAVIILGAPESANDELAHLKDEMRLIRNAVQKEIPVLGICLGSQLIAKAFGAMVYQGK
                     KKEIGIYHDIEFDNTSKSELFAGIKSPATVFHWHGDTFNLPQGAIRLAHSANYENQAF
                     QIGTAVGVQFHLEVDEATIRLWLEKSKEELSQLPYIEPDKIIKQIPEHIQTIQNNMKI
                     FYRNFKAAYNL"
     gene            5575..>5781
                     /locus_tag="VLB45_04755"
     CDS             5575..>5781
                     /locus_tag="VLB45_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010193324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HSD05047.1"
                     /translation="MSSITKVFEETIVTDHKVITEELSKSVLKKYGVKVPGYALVNSA
                     KEATKAAKKLGYPLVMKVVSPQILH"
BASE COUNT         1767 a         1135 c         1138 g         1741 t
ORIGIN      
        1 tttatcaatg cactgaaaaa ttctctggaa ggtggtcagg aaaacaagcc aaaaggaatg
       61 ctgcttacag caacgcttac tgagataaag ggtggaacaa gccacaatgt aacagctata
      121 gaatgcgatg cgataatgga cattaggatt ccagttgaca tgaattgcaa aatggttgga
      181 gaaaaaattg caacaacggt acaagagatt tcaaaaaaac aaggagtcag cgcgttttat
      241 tctgtactgg atgagactga accatttgag gcaccgcata catcaccgct tgtcagagct
      301 ctgacacttg gagtactaga tgtagaaaaa aacaggccgc aattgatacg caagactgga
      361 actggagaca tgaacataat tggaaatgcg cttaacatcc ctgttgtcac gtatggtcct
      421 ggagatcccc atgcatcaca tactattgac gaacgagtgt cagttgatga atatctaaga
      481 agcatcgagg tcttcaagca catgctgtac cacttgaaga gactgcatgt aaaatcaaag
      541 taattcaaaa tctcgcaagc tcaaaataac caacttttgg catttcaatc atgctttggt
      601 ttgcaggttt aggaatctcc ggaactgacg gaatgggttt ggatgtagta aaaattctca
      661 agcaggcaga tattgtatat ctagaaattt ttaccagccc aattacaaag ccagagctag
      721 caaagataaa aaaactagtc aaaggcaaat tcaagaccgc accaagatgg ttagttgaag
      781 atggaaaagc aatacttgaa gatgccaaaa gaaaagaagt ggtgcttctc tcgtatggtg
      841 acccttacat cgctacaacc cacattgagt tgcgcacaag agcaatttca gaaaagatca
      901 agacagggac catccatgca gcatctgcta ttacgtctct tgttggagaa tgcggattac
      961 actattacaa gataggcaaa attgtcacca tgatgcaaga gcaacagtct gcatccagcg
     1021 tgtattatac cacttatgat aatctagtgg ctggcaacca caccattata gttttagagt
     1081 acaaccaaga tgccaagttt ttcttgaatc caaaagatgc actaaataat ttacttttga
     1141 cagagagtgg acaaaaaaga aacgtggtgg acgagtcaag ctttgcaatt gttgcatcaa
     1201 ggattggatc aaagacacaa aaaattgttg caggaaaatt ctctagttta aaaaagacag
     1261 actttggcaa gccgccacac agcataatca ttccaggaag aatgcacttt acagaatctg
     1321 atgcactcaa agtccttgca agatgtcttg acgagccttt tgacaactct tctagaatta
     1381 aaaaaatttc agatcaaatg ctagaaaaat atattccaaa agcaagaaag gcacttgaag
     1441 acgtgacaaa caggttcaag gatgacaagg gcatcatgtc tgttttggaa aatgcagagc
     1501 tataccttga tgatgcagaa aaatttcaaa gccaaggaaa tgaggagctt gcagtcctga
     1561 gcatagggta tgcagagggg ctgatagacg cactgcgttt ttcaaaagga atagatccat
     1621 gggcacagag tttatagatg aaataaagac taggaatctt gttttagtga tatagttgga
     1681 tgctttagac aaggaaataa tttcggcctt gtatgtctcc tcactttcag acgaggatcc
     1741 gatttccata atcaggagaa aacttcctgt gaatccagac cacctaaaag caaagataga
     1801 ctttcttgca aaaaatggat ttgtagaaga ggacaaaaaa acactaacag atattggcag
     1861 atcctcgatt cgtgtggttc ttgctggtgg tgtctttgat atcatacatc caggccattt
     1921 gcacaccctg aggggagcaa aggcgcttgg caatgtactg attgtagtaa tcgcgacaga
     1981 ccaaactgcc cagaagatga aaagcagaat acctcttcac aacatggaat tgcgtcgtga
     2041 tctggtaaat tcactatcaa tggtagatta tgccgttgta ggacatgagg gagacatttt
     2101 caaaacagtt gcgcgcgtca agccggatat aattgcacta ggctatgatc aggtgcatca
     2161 ggaaaaattc atcactgatg gatgcaaaaa aatcaatctt agtgtaagca tagcaagact
     2221 ccagtctcct attccagaca ttaaaagctc aaagatagaa agcgagtacg gcgaatcaat
     2281 ttacagaatt tagtatcgtt tttcgttttc acatattggg catttcagac cgtttgtctt
     2341 aaaaccacac ttggtgcact tgagtgacac aaaacttctt tttgacgatt ttattatttt
     2401 gtaaatcaga gcaactgtaa ttatagttcc tactgcaatt ccaatccaaa ttagaaccat
     2461 cagtatatga tatggctttc atccataatt agcttggctt gtcgttattt agaatgcata
     2521 ggcacgcgta ttgagatctg tgagccagta gaaagttttg ctgccttttt gatactagtt
     2581 tgagagatca actcgattat ggaatcatca taatgtgtcc tttctagagt tatcatcatg
     2641 ccgtctacag agttgtttat ttttgcagga taacatttta cccatccata ggttcttttt
     2701 ccatcagaaa atccgtttat cattattcct tggtgcgtat caagttcgcg cttggcctcg
     2761 gtgaattttt tatcatttag tttgacatta agtgtgcctg gaaatgggac atatcccagt
     2821 tttgatttga attgttttgt gtatcctttc attgacatgt agtatgcgcc ttcacccatt
     2881 ccagaaatta ttgttccctt gaactcaata tacgatggtg atgactctag acttgatttt
     2941 aaaattgaag agatctttgt tatctctgcc tgtcccttgc ttgtgattct gactgaaact
     3001 ttttgtccgt ttcttattct ttctatgtat ccatcacgct ccaattccaa aagatgtttt
     3061 gatgcggcct gctgtgatct gtttatactt ttaccaaggg atgtagtggt tatagaaaca
     3121 aagttgtgtc ttgcaccttt tgataaaagt tcagcaagag tcaggatgtg ctgtaccttc
     3181 agttccgaca ttgaagagtt aggctacgcc gagctctgta aatgttttga gtatcagtcc
     3241 gtctgtgttt ttgaggtttg ccattcgatg gcctatcacg tactctactc ctgcttgctt
     3301 tgcagcatca atcaatcttt gagttatgat tccgtcaagt acaagatatt ttattccaga
     3361 acctgaagtg atttttccta caacttcgct tactggaact ttgaagagct cgttgagacc
     3421 atcatctagt gctactgctt caagtgattc gtttagattt tcatatacct tttttgcaac
     3481 atttgagatt gcttcgtctt ttggatcatg cagtcttggt tttgccgttt cttttttcat
     3541 gtcctctgca gtttctttta gaatgtctgc aatctgttgt ggcgttagct cctcaacttc
     3601 aactccttct ggtgcacgat gaactacatc gacatgaacc attgccttga gttcttttag
     3661 tatgaatcca ccagctctgt ctccgtccaa gaatgcaacg actttggatt ttgattcaca
     3721 taattccttt attgattcat caattcttgc accttctatt gcaagtgcat tatcaaaacc
     3781 agctcttagc aagtttatga catcagctct tccttcaact agaattatcc aaggcgaatc
     3841 aaaaactccc gagccgcatg caagtttttc tcttccaaat gtggcaagtt ttcctgaaga
     3901 acctccttct gaaacatcct ttagcatctc ttctccttcg ctgattgttt ttgtagacca
     3961 tttctgaaca atttcttttg ctctatcaac aatctctttt ttctttgcag aacgaacatc
     4021 atcaatagct tctagtttga aatgagcttg aaatggacca accttgtcta tgctttctac
     4081 tgcagcagcg ataagagcag ctgtagttat gtctgtgctc ataggcatca aagcatctcc
     4141 tttagtttga tttgacgtgg ccgttgtgtt aacttctatc ctaccgacct tcgaaacttt
     4201 ctgcagttcg tttaggttca tctctggtcc taacagtcct tctgtttggc caaaaattgc
     4261 gcctatgatg tcggcttttt caaccaatcc atcgacgtca aagctgagct taacgtgata
     4321 cttgacaatt cctgtacttg gcaattcata atcctcttaa tttttgatca atttcttttc
     4381 ccaaaagacg gatataagtg cttatgatga cgggattgat cttatcggca attcgtatct
     4441 tgagagatcc tcaatatgtc gtatttttcc cttggttatt gcaacaagtt tcttcttaaa
     4501 agaaagatcg atttttgttc tatgctcaag caatttgatt attctgcttg taagatatct
     4561 gccttttcga tcaagatcaa gtaagagtac gagtttcttg tatgaatcca ttgaatctgc
     4621 aaatcttgtt aatcccttga agctgtgaaa ttcgcatatt gttccacgat agccaagttt
     4681 tttgagcgcc gtagaatcac gttttccttc aaccacgaca acactgtctt ttgagtttag
     4741 tgaagctatg aaatcttgca gagcattgat ctcgtcttct gtaatcttca cgactaactt
     4801 tacatcttga catattaagc agtttttagc gaatatagta actgtgtccg atattcttgt
     4861 cttacaaaat gcaaaatatg aagggcttgc tgggcttggc aggctgttag cagaggatgg
     4921 gtttaaagta aaaactgtaa ttgcaaagaa ggaaaaaatc ccgtttcctg attcatcagc
     4981 agtaataatt ttaggagcac ctgaaagtgc aaatgatgag ctagcacatc ttaaagacga
     5041 gatgcgattg atcagaaatg ccgtacaaaa agagattcca gtacttggga tctgtcttgg
     5101 atctcagctt attgcaaagg catttggcgc catggtctac cagggaaaga aaaaggaaat
     5161 agggatatac catgatattg agtttgacaa cacatcaaag tcagaacttt ttgcaggaat
     5221 caagagtcca gcgacggtat ttcattggca tggagatacg ttcaacctac cacaaggtgc
     5281 aataagacta gcccactctg caaattatga aaaccaagca ttccagatag gaacagccgt
     5341 tggggtgcag tttcatctag aagtggatga agcaaccatc aggctatggc tagaaaaatc
     5401 aaaagaagaa ctaagtcagt tgccatacat agaaccagac aagataataa aacagattcc
     5461 agagcacata caaaccatac aaaataacat gaaaattttt taccggaact tcaaggcagc
     5521 atacaatctt tgacaaaagt ttaatataat gacggctaga cttttcgatc tggcatgagt
     5581 tctataacca aagtctttga agaaacaatt gttaccgatc acaaagttat caccgaagag
     5641 ctttcgaaat ctgttctgaa aaaatatggt gtaaaggttc caggatatgc acttgtaaat
     5701 tctgccaaag aagctacaaa ggctgcaaaa aaacttggct atccacttgt catgaaagtt
     5761 gtatcaccac aaatacttca c
//
LOCUS       DATIDL010000106         9787 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrosopumilaceae archaeon isolate SMAG_U16342
            k141_2759280, whole genome shotgun sequence.
ACCESSION   DATIDL010000106 DATIDL010000000
VERSION     DATIDL010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935836
            Sequence Read Archive: SRR25158326
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosopumilaceae archaeon (soil metagenome)
  ORGANISM  Nitrosopumilaceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrosopumilales;
            Nitrosopumilaceae.
REFERENCE   1  (bases 1 to 9787)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9787)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/04/2024 05:09:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,520
            CDSs (total)                 :: 1,474
            Genes (coding)               :: 1,474
            CDSs (with protein)          :: 1,474
            Genes (RNA)                  :: 46
            rRNAs                        :: 1 (5S)
            complete rRNAs               :: 1 (5S)
            tRNAs                        :: 43
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9787
                     /organism="Nitrosopumilaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2759280"
                     /isolate="SMAG_U16342"
                     /isolation_source="soil"
                     /db_xref="taxon:2202732"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..693
                     /locus_tag="VLB45_04760"
     CDS             <1..693
                     /locus_tag="VLB45_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HSD05048.1"
                     /translation="WRVPAELNEDEKRNLLQTISKFTSGKNATEIMDELVGYTYTLSG
                     EDKRSFLRDAREFSTMLNSCGRIRKAGVGVAICMGDRTKILEEGEGILLEYRTVLRTY
                     MNTLASERWRVVDNGQYLMVNGEGLVSENMTGAISSLLGGSQKNAGKIIILRTNGDEG
                     TIKFSSRKSTGCKSEVNLGLLMRACAAKVSGVGGGHDAAAGARITKDKLDEFLDCLEE
                     NVSRMQSSGLSK"
     gene            656..898
                     /locus_tag="VLB45_04765"
     CDS             656..898
                     /locus_tag="VLB45_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KEOPS complex subunit Pcc1"
                     /protein_id="HSD05049.1"
                     /translation="MSLECKVQVFLNNLTPQKADTIRKALEPDNVDFPKNLSLEIENV
                     DNSLVFTFQGKGNIRNLISTIDEVLEQTQVVLRVTS"
     gene            898..2160
                     /gene="serS"
                     /locus_tag="VLB45_04770"
     CDS             898..2160
                     /gene="serS"
                     /locus_tag="VLB45_04770"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HSD05050.1"
                     /translation="MLDPKILRETPEKIRQMLKDRVVDFPLDELIKLDKERRELIIKT
                     DEIRKKKNEIALEIAQKKKSDQDATLLIEQMRQVSDTLSGLENTQTKTESEYARLSLT
                     IPNLVHESVPIGKDETANKEIKKWGKIPQFDFKVKDHIDISQNLDLVDLERAAKVAGA
                     RFYYLKNDLVKLNQALIQYALDFLSEKNYTPIQTPFMINKSSMEGAIIASDFEDVIYK
                     IEGDDLYLIGTSEHAVVSMHADEILDGKNLPLRYAGVSPCFRKEAGAHGRDQKGIFRV
                     HQFEKVEQFVFARPEESWKEHERMLSLVEEFYQNLEIPYKIVILSSGDLGKISAKTYD
                     LEAWMAGQNSYREIVSCSNCLDFQARRLKIRFRDRTNDQPQYLHTLNSTLVATSRTLV
                     SILENFQTKDGHVTIPKILQKYVGKNTI"
     gene            2203..2817
                     /locus_tag="VLB45_04775"
     CDS             2203..2817
                     /locus_tag="VLB45_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3ae"
                     /protein_id="HSD05051.1"
                     /translation="MARQKTARVKDKWREKKWVTVLSPSAFNNVPIAYIPLTDDASGI
                     GRVVEVTLFDIVKGDPSQHQYKLYFQVNKVDGETATTIFKRYEYAKEFLRSLVRRGSS
                     LINFIGDYKTKDGYIFRIKVIALTQKKLNTSRKHALRLIARDVMAKTIPEMTIDQFVQ
                     ATAFGKINSDIMAAVKKIIHVRHIGIEKAKIIRTAEAEIALLQA"
     gene            2850..3272
                     /locus_tag="VLB45_04780"
     CDS             2850..3272
                     /locus_tag="VLB45_04780"
                     /inference="COORDINATES: protein motif:HMM:NF013994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding domain-containing protein"
                     /protein_id="HSD05052.1"
                     /translation="MVNHIETTAEVIIHATEDKKKILEPLLEMFEIEEEEFSEENLDG
                     HFGNPILLLKIKLVKKRAEQFIKKVISKMSQTQINEFLKDIEMHFEDSSLYIRIAKQD
                     MVRKQINLQQTDAIKIKISVPVYKKSETVATYLELLKP"
     gene            3269..3481
                     /locus_tag="VLB45_04785"
     CDS             3269..3481
                     /locus_tag="VLB45_04785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05053.1"
                     /translation="MRKKKRYILVQLENNTNLGNFSDIKIISNEGSHAIVLCQLDKLT
                     QVVSELEKQCKIVTVSGTLKSIRSSV"
     gene            3628..5079
                     /locus_tag="VLB45_04790"
     CDS             3628..5079
                     /locus_tag="VLB45_04790"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HSD05054.1"
                     /translation="MKTDYDIIVAGGGLAGMIAAQSASFYSKQGLSILVIDRNPEPTL
                     GKKTISGWVCGDAVGKNTVDYMTERIKISWGSPEIEHPVKGVMAFSPDRETSIPFDGD
                     GYMLNRQMLPRRQLADAKKIGIVMQYSVALRNLLYENDTVVGVEGTDLLTNQPYKKTA
                     KIVIDATGVASMLRNGISIKTKIERKIDRDDLEATGRHIMKFENGVEDKTDFDPDYCI
                     IHLDQDIAPGGYGWVFPKGKNKVNIGLGVQQKELQRRNGRLGKKDDVMKLINDYVKIN
                     KAIKNPKLSDDQSDSINATGTWQVSVRRQNDCLVANGFMLVGDSAWMPKPLDAGGIGP
                     AIVAATIIGKNAVAAIEANDVSEKGLWQYNIDFINDYGYKTAGLEVFRRLLQTLTNDQ
                     INYGMKHFLSKMDVESISKGEHPDFGTVSKLGMLIRGALNKELAEGLRFTAQQNKILT
                     EHYYNFPKTPDGFDAWNHTLHRILDESFAKIKN"
     gene            complement(5076..5768)
                     /locus_tag="VLB45_04795"
     CDS             complement(5076..5768)
                     /locus_tag="VLB45_04795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05055.1"
                     /translation="MMQIKIKFLLTSFILLSSMLHAAYSEEIKIPDWVRNNAKWWSNG
                     EIDDKTFAGGIQYMIKEGIISVSVDSSQNSSDELDMSKCDQFTTAAEKRTCQKEIELA
                     NKIKNDIEKSTSYVIGPVTFYYVDDKIEKTSQGGTILTIYFIVENTGGSHDITMSCPV
                     NACNYVLSDGQKEIQYTTNTLVYGSLTLQPHSPELLEWSFYKGLDYDPTKDYFFKVKE
                     SWGNGSIPLEID"
     gene            5870..6667
                     /locus_tag="VLB45_04800"
     CDS             5870..6667
                     /locus_tag="VLB45_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007551355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HSD05056.1"
                     /translation="MLTESLYIDWNNSFEILDKAYESGLFVLIIGPKGTGKTSLVREF
                     ATRKGVNLESVNFSLRTRESHLVGSKSLENGSIGFDAGILVKSMKEGNMLYLDEINAA
                     EADVLLRLDEALDDRRQLVLKESGGELIQANKEWFVVATINPLTHVGTKELPPQLLSR
                     FPVRIRLEYPPEEIELQIIKKHISGSHDKEILQGIKLANLLRQAAAVEELYYSPSLRE
                     TIAFGKLLDAGLKPRHAADIVFANVYAQWGNVEYQKVNDIITSMFGN"
     gene            6667..8232
                     /locus_tag="VLB45_04805"
     CDS             6667..8232
                     /locus_tag="VLB45_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HSD05057.1"
                     /translation="MQSLHLRNDTILEIATFLVRRWSEKQNITIGITEQKDIQTKIKE
                     NKIVMFPLDHYFGTDFQKYRQFRTALWYEAMRLKHCTKILSNDHAFGFLLNTLETRRI
                     ETVGRKQWQGMDEEIILSYGFAWLYRPLLNSLYGKSRIVEGFSQYFLMGDIKGEVTPS
                     QFEKIIKATEFSKEVVKEAIEKNHDTEWLEKKIPEIIKILEIDALLTIPLSVPKISSG
                     IAMSEEDFVKTLGKIAKFHESDFGELDPKKISEGKTVFEEFKVLLEENQLNENRGLRT
                     ENIGISIPSSMDVDESKIYDADLVSNLKTKFKEWKTGWKEQHVSSGDEFDEETYMEGH
                     TPFFTDKKITIKTRIVILLDHSSSIANEQIQYKKATLALCEVLAFLKVKFSVYAFNTE
                     QRQVTCWRVKPENVKWNRIAAKRLAQIKANGGTPLAEVYSIMLPILRSKKPDIFLTLS
                     DGEPSDPDSVRSMVHTFKLLGIKMVAIGVGPSPTISTIIASNLKHLGYENTLAVSRLN
                     DIPNRVLNVLGRN"
     gene            8234..8689
                     /locus_tag="VLB45_04810"
     CDS             8234..8689
                     /locus_tag="VLB45_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010194869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HSD05058.1"
                     /translation="MKCLSVSQPYADLIIQGKKTIELRTWNTKFRGEFLVHAPFKIKK
                     DVCKKLEIDETKIRTGVIIGKVEIYDVKVYNSVSELKSDYKKHFATEEFLCHKYGFLL
                     KKPQELRIPIPYKGSLGFFDVKLDTKIKPNEIKTNLFDEEYRYQWIGRH"
     gene            complement(8686..8913)
                     /locus_tag="VLB45_04815"
     CDS             complement(8686..8913)
                     /locus_tag="VLB45_04815"
                     /inference="COORDINATES: protein motif:HMM:NF013587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AN1-type zinc finger protein"
                     /protein_id="HSD05059.1"
                     /translation="MQPSTCQYCGEISDLPFECSYCKDNFCHDHRLPEEHRCVKLRLI
                     RAKRFGQQNVIRDGNRQRPNPLRQMLSRFGI"
     gene            complement(8958..9623)
                     /gene="fsa"
                     /locus_tag="VLB45_04820"
     CDS             complement(8958..9623)
                     /gene="fsa"
                     /locus_tag="VLB45_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HSD05060.1"
                     /translation="MKIFLDTANIAAIKLYNDMGLVDGITTNPSLLAKEGGDPQKTME
                     EIVRIVKGDVSLEVVATDTAGMIEEGRRLRKYGKNVVVKCPLTPDGLKACRTLTDEGI
                     PVNVTLCFSANQAVLAAKSGAKYVSPFIGRLDDAGQDGMGLIKEIHQIFKNYQFSTQI
                     LVASIRHPMHVVDAGKIGADVVTLPPDILGKMLKHPLTDIGLKNFLADWEKLKKENPS
                     IRI"
BASE COUNT         3434 a         1651 c         1934 g         2768 t
ORIGIN      
        1 tggcgtgttc ctgctgagct aaatgaggat gaaaaaagaa atctcttgca gacgatttcc
       61 aaatttacgt ctggaaaaaa cgcaactgag ataatggatg agcttgtagg atacacttac
      121 acactttctg gagaagacaa acgcagcttt ttgcgagatg ctagagaatt ttccacaatg
      181 ctaaactcct gtggaagaat tcgcaaggca ggagttggag ttgccatatg catgggagac
      241 aggactaaaa tacttgagga gggagagggc atcctcctag aatatcgtac agttttgagg
      301 acatacatga acactcttgc aagtgaacgc tggagagttg tagataatgg tcaatacctg
      361 atggtaaatg gagagggttt ggtttcagaa aatatgacag gtgcaatttc atcacttctt
      421 ggcggttcac aaaaaaatgc tggaaaaata atcatacttc gcacaaatgg agatgaaggt
      481 acgatcaagt tttcatcacg aaaatccaca gggtgcaaat cagaagttaa tttgggtctg
      541 ttaatgagag cttgtgcagc caaagtttct ggtgttggcg gtggtcacga tgcagcagca
      601 ggtgctagaa taactaaaga caaattggac gagtttttag attgtttaga agaaaatgtc
      661 tctagaatgc aaagttcagg tctttctaaa taatttaaca ccccaaaaag cagatactat
      721 acgcaaagca ttagaacctg acaatgtaga ttttccaaaa aatctttcgt tagagattga
      781 aaatgttgac aactcacttg tctttacttt tcaaggtaag ggaaatatac gaaatcttat
      841 ttctactatt gatgaagtcc ttgaacagac tcaggttgta ctcagagtga ctagttgatg
      901 cttgatccta aaattcttcg agagactcct gaaaaaataa ggcaaatgct caaggataga
      961 gtagttgatt ttccacttga tgaattaatt aaattagata aggaaagaag agagttaatc
     1021 ataaaaacag atgagattcg caagaaaaaa aatgagattg cattagaaat tgcacagaaa
     1081 aaaaagtcgg atcaagatgc caccttactg attgaacaaa tgagacaggt ttcagataca
     1141 ctatcaggac ttgaaaatac acaaaccaaa acggaatcag aatatgccag actttcactt
     1201 acaattccca atctagtaca cgaatcagtt ccaataggaa aagatgaaac cgcaaacaaa
     1261 gaaataaaaa aatggggaaa gatcccgcag tttgatttta aagtaaaaga ccacatcgac
     1321 atttcacaaa atctagatct tgtagattta gaaagggctg caaaagttgc tggcgctaga
     1381 ttttactatt taaaaaatga tcttgtaaaa ctaaatcagg cattaatcca atatgctttg
     1441 gactttcttt ctgaaaaaaa ctatactcct atccagactc catttatgat aaacaaaagt
     1501 tcaatggaag gtgcaataat tgcaagcgat tttgaagatg tgatttacaa aattgaggga
     1561 gatgacctgt atctaattgg aacatcagaa catgcagtag tatcaatgca tgctgatgaa
     1621 attcttgatg gaaagaactt accgctaaga tatgctggtg ttagtccttg ttttagaaaa
     1681 gaagcaggtg cacatggaag agatcaaaaa ggaatttttc gtgtgcatca atttgaaaaa
     1741 gtggaacagt ttgtttttgc cagaccagaa gaatcttgga aagagcatga aagaatgctt
     1801 tctctggttg aagaatttta ccaaaatcta gagattccat acaagatagt gatcttatca
     1861 agcggtgacc ttggaaaaat ttctgccaag acttatgatc ttgaggcgtg gatggcagga
     1921 cagaatagct atcgagaaat tgtctcatgt tctaattgtc ttgactttca agcaagaaga
     1981 ctcaagatca gatttcgaga taggacaaat gatcaaccac aatatcttca cacattaaac
     2041 agcacattag tagctacatc acgaacactt gtttccatac tagaaaattt ccaaacaaaa
     2101 gatggtcatg taacaatacc aaagattttg caaaaatacg ttggcaaaaa caccatctaa
     2161 aagtcgtaca acttatattt tggctacaaa cgtcgatcat agttggcacg tcaaaagact
     2221 gcaagagtaa aggacaagtg gagagagaag aaatgggtta ctgtactttc tccttctgca
     2281 tttaacaacg ttccaattgc ttacattcca ttaacagatg atgcaagcgg cataggccgt
     2341 gttgttgaag ttacattgtt cgatattgta aaaggagatc cttcacaaca ccaatacaag
     2401 ttgtattttc aggtaaacaa ggtagatggc gaaactgcca caactatttt caaaagatac
     2461 gaatatgcaa aagaattctt acgaagtctt gtaagacgag gctcctcact gatcaatttc
     2521 ataggagatt acaagacaaa agatggatat atttttagaa tcaaagttat tgcgcttacg
     2581 caaaaaaaac taaacacatc acgtaaacac gcactcagat tgattgctcg tgatgtcatg
     2641 gcaaaaacaa tacctgaaat gacaatagat cagtttgtac aagctactgc atttggaaag
     2701 ataaattctg acatcatggc agcagtcaag aagataattc atgtaagaca tattggaatt
     2761 gagaaggcaa agataatcag gacagcagaa gctgaaattg ccttgctaca agcctaatag
     2821 gtttattctt tgcataatat caaaagaaca tggtaaatca tatagaaacc acagccgaag
     2881 tcatcataca tgcaacagaa gacaagaaaa aaattctaga accattactt gagatgtttg
     2941 agatagaaga ggaagagttt tctgaagaaa acctagatgg ccactttgga aatcccatat
     3001 tattgctaaa aataaagctt gtaaaaaaaa gagcagaaca gttcatcaaa aaggtgattt
     3061 caaaaatgtc tcaaacccag ataaatgaat ttttaaaaga cattgaaatg cattttgaag
     3121 attcatcatt gtatattaga atagcaaaac aagatatggt gaggaaacag attaatttac
     3181 aacaaaccga tgcaataaag atcaaaattt cagttccagt gtataaaaag agtgagaccg
     3241 tagcaacata cttggagctt ttaaagcctt gagaaagaaa aaacgataca tcttagtaca
     3301 attagaaaat aatacaaacc ttggaaattt ttcagacatc aagataatct caaatgaagg
     3361 aagtcatgcc attgttttgt gtcagctgga caaactcaca caagttgtat cagaacttga
     3421 gaaacaatgt aaaatcgtga ctgtgtctgg cactctaaaa tcaatacgaa gtagcgttta
     3481 atagtattct agcagcaaag aaaaagggga tgagggtttg ccgctccagt ctgagcatcc
     3541 gctgtgaaga agccgcgacg aactgaccca aaaattgatc taactctgca cggttctttt
     3601 aaatatagta gatcctgtgt tttcgctgtg aaaacagatt acgacatcat agtagctgga
     3661 ggtggacttg ctggaatgat tgctgcacaa tctgcttcgt tttactctaa acaaggatta
     3721 tctattctag tgattgacag aaatccagaa ccaaccctag gtaaaaaaac aatcagtggg
     3781 tgggtttgtg gagacgctgt aggaaaaaac acagtagact acatgacaga gagaatcaaa
     3841 atatcatggg gtagtccaga aatcgagcat cctgtaaagg gggtaatggc attttctcca
     3901 gatagagaaa catccattcc atttgatggg gatggataca tgctaaacag gcagatgcta
     3961 ccacgacgac aacttgctga tgcaaaaaaa attggaattg tgatgcaata ctcggtagca
     4021 ttacgaaatt tgttgtacga aaatgataca gtagtaggag tagaaggaac cgatcttctc
     4081 accaatcaac catacaagaa aaccgctaaa attgtgattg atgcaactgg tgttgcctct
     4141 atgttacgaa atggtatttc tataaaaaca aagattgaga gaaaaatcga tcgtgatgat
     4201 ttagaagcaa cgggaagaca cattatgaag tttgaaaacg gagtagaaga caagacggat
     4261 tttgatcctg attattgtat aatacattta gatcaagata tagcacctgg aggatacgga
     4321 tgggtctttc caaaaggcaa gaacaaggta aacataggtt tgggcgtgca gcaaaaggag
     4381 ttacaaagaa gaaatgggag attaggtaaa aaagatgatg ttatgaaact gataaatgac
     4441 tatgttaaga tcaacaaggc aataaagaac ccaaaactgt ctgatgatca aagcgattcc
     4501 atcaatgcta ctggcacatg gcaagtttct gtaagaagac aaaatgactg tcttgtagct
     4561 aacggattca tgcttgtagg agattctgca tggatgccaa aaccgcttga tgcgggagga
     4621 attggtcctg ccatagttgc agcaacaatc attggaaaaa atgcggtggc tgcaatagaa
     4681 gctaatgatg ttagcgaaaa aggattgtgg caatataata tagatttcat aaacgattat
     4741 ggatacaaaa ctgcaggtct tgaagtcttt agaagattat tacagacact cacaaatgat
     4801 cagataaact atggcatgaa acactttttg tcaaaaatgg atgttgagtc aataagcaag
     4861 ggagaacatc cagactttgg gacagttagc aagcttggca tgttaattcg cggtgcatta
     4921 aacaaagaac ttgcggaagg gctcagattt actgcgcaac aaaacaagat tttgacagag
     4981 cattattata attttcccaa gactcctgat ggatttgatg catggaatca tacgctgcac
     5041 aggatcttag acgaatcttt tgcaaaaatc aaaaactagt caatttctag tggtatagat
     5101 ccattgcccc aagactcttt tactttaaaa aaataatctt ttgtaggatc gtaatccaat
     5161 cccttataga aactccattc taatagttct ggggaatgtg gctgtaaagt caaagaacca
     5221 tacacgagag tgtttgtagt gtattggatt tctttttgtc cgtcgcttag tacataatta
     5281 caagcgttta ctgggcatga cattgttatg tcatgagagc caccagtatt ttcaacaata
     5341 aaatagattg taagaatggt tcctccttga gatgtttttt cgatcttgtc atccacatag
     5401 tagaaagtta caggcccaat tacatacgaa gtggattttt caatatcgtt tttgatctta
     5461 ttagcaagct caatttcttt ttggcatgtt cgcttttctg cagcagtagt aaactgatca
     5521 cactttgaca tgtctagttc atctgaagag ttttgagaac tatcaacaga tacagaaatt
     5581 attccttctt tgatcatata ctggattcca cctgcaaatg tcttgtcatc gatttcaccg
     5641 tttgaccacc acttggcgtt gtttctaacc caatcaggga ttttgatttc ttcggagtat
     5701 gctgcatgta acatactgct taaaagaatg aaacttgtta gcagaaattt tattttaatc
     5761 tgcatcatga tatcatatcc tttctttcag attaacttga tcgtcgcaat agtccatgga
     5821 agaccaagta agactatttt taaattaagg tcgcgttctc ccaaaggtat tgcttacaga
     5881 atcgctttac attgactgga ataattcctt tgagatttta gacaaggctt atgaatctgg
     5941 cttgtttgta ttaatcatag gcccaaaagg aacaggtaag acaagccttg ttcgtgaatt
     6001 tgctacaaga aaaggagtca atcttgaatc ggttaacttt agcttgagaa cgcgcgagag
     6061 tcatcttgta ggttcgaaga gtttggaaaa tggttctatt ggttttgatg caggaatact
     6121 ggtaaaatct atgaaagaag gaaacatgct ttaccttgat gagataaatg cagcagaagc
     6181 agacgtgcta cttagattag atgaagcatt agacgacaga agacagcttg ttctaaagga
     6241 atcaggagga gaattgattc aagcaaacaa agaatggttt gttgttgcaa caataaatcc
     6301 actgacgcat gttggaacaa aagagctacc accacaactt ctaagcaggt ttccagtcag
     6361 aataagatta gaatatccac cagaagaaat tgaacttcaa attataaaaa aacacatctc
     6421 tggttctcat gacaaagaga tcttgcaagg aatcaaactt gcaaatttgt tacgacaagc
     6481 tgctgcagtt gaagaattgt attattctcc aagcttacga gaaacaattg catttggtaa
     6541 gcttcttgac gcaggtctaa aaccaagaca tgcagctgat attgtatttg caaatgtata
     6601 tgcacagtgg ggaaatgtag aatatcaaaa agtcaatgac atcattacgt caatgtttgg
     6661 taactaatgc aatcattaca tcttagaaat gataccattc ttgaaattgc tacctttctt
     6721 gtcaggagat ggtctgaaaa acaaaacatc acaataggaa tcacagaaca aaaagacatc
     6781 cagacaaaga taaaggaaaa caagattgtc atgtttccct tagatcatta ttttggaact
     6841 gactttcaaa aatataggca gtttagaacc gctttatggt atgaagcaat gagactaaaa
     6901 cactgtacca aaatactaag taatgatcat gccttcggtt ttttactaaa tacccttgag
     6961 acaagacgaa ttgaaacagt aggcagaaaa cagtggcaag gcatggatga ggaaataata
     7021 ctcagttatg gatttgcttg gctttacaga ccgttattga attcgctata tggaaaatca
     7081 agaattgttg aagggttttc acaatatttt ttgatgggcg acatcaaggg tgaagtaaca
     7141 ccaagccagt ttgaaaaaat tatcaaggca acagagtttt caaaagaagt tgtcaaagaa
     7201 gcaatagaaa aaaaccatga cacagaatgg cttgagaaaa aaattccaga aattataaaa
     7261 attttagaaa ttgatgcatt attgacaatt ccattatcag ttccaaagat tagctctggc
     7321 attgcaatgt cagaagaaga ctttgtcaag acgttgggta agattgcaaa gtttcatgaa
     7381 agcgatttcg gtgagcttga tccaaagaaa atttctgaag gaaaaacagt atttgaagaa
     7441 ttcaaagtgt tattggaaga aaatcaacta aatgaaaatc gaggtttgcg tactgaaaac
     7501 attggaataa gtattccaag ttctatggat gtagacgaat caaagatcta tgatgcagat
     7561 cttgtaagca atctcaagac caaattcaaa gaatggaaaa ctggttggaa agaacaacac
     7621 gttagctcag gtgatgagtt tgacgaagaa acatacatgg aaggacacac cccatttttt
     7681 acagacaaaa aaatcacaat caaaacaaga attgtgatat tgcttgatca ttcctcaagc
     7741 atagcaaacg agcaaataca gtacaaaaaa gccacgcttg ccttatgcga ggtacttgcg
     7801 tttctgaagg taaaattttc agtttatgca ttcaatacag aacaaagaca ggtaacatgt
     7861 tggcgcgtca agcctgagaa tgttaaatgg aatcgtattg cagcaaaaag attagctcaa
     7921 ataaaagcaa acggcggaac tccacttgca gaagtttatt caataatgtt accaatattg
     7981 cgttcaaaaa agccagacat ctttcttact ctgtcagatg gagaaccatc tgatcctgat
     8041 tcagtacgtt caatggttca cacgttcaag ttgttgggaa taaagatggt ggcaatagga
     8101 gttggaccta gcccaaccat atcaacaata attgcaagta atctaaaaca tttagggtat
     8161 gaaaatactt tggctgtaag tagattaaat gacataccaa acagagtatt gaatgtgtta
     8221 ggaagaaact agaatgaagt gtctgtcggt gtcacagcct tatgccgatc ttataataca
     8281 gggtaaaaaa acaatcgagc ttcgaacttg gaatacaaaa tttcgcggtg aatttcttgt
     8341 ccatgcacca tttaaaataa aaaaagatgt atgcaaaaaa ctagaaattg atgaaaccaa
     8401 aatcagaact ggggttataa taggtaaagt tgagatctat gatgtcaaag tatacaattc
     8461 agtgtctgaa ttaaaatcag attacaagaa acattttgct acagaagaat ttttgtgcca
     8521 caagtatggg tttctgctta aaaaaccgca agaattgaga ataccaattc cttacaaggg
     8581 tagtcttgga ttttttgatg ttaaactaga tactaaaata aaaccaaatg agatcaagac
     8641 gaacttattt gatgaggaat atagatatca gtggataggc aggcattaga ttccaaaccg
     8701 agaaagcatt tgtctcaaag gatttggtct ttgtctattg ccgtcccgta tgacattttg
     8761 ttgcccaaac ctttttgctc taattagtct tagttttaca catctgtgct cttcaggcaa
     8821 cctatgatca tggcagaaat tatccttgca ataactgcat tcaaaaggca agtctgatat
     8881 ttcgccacag tattggcatg tactaggttg catgcaatag atctcttttc atattataaa
     8941 atggtttttt gtttgaatta gattctgatg cttgggtttt ctttctttag tttttcccag
     9001 tcagctaaaa agttcttcag accaatatca gttagtgggt gttttagcat ctttcccaga
     9061 atgtctggag gtaatgttac tacatctgca ccaatctttc ctgcatcaac aacatgcatt
     9121 gggtgtctga tacttgctac tagtatttgt gtactaaatt ggtaattctt gaagatctgg
     9181 tggatctctt tgatcaatcc cataccgtct tgacctgcat catccagtct tccaatgaat
     9241 ggactgacgt attttgcacc agattttgca gctaaaactg cctgatttgc tgagaaacat
     9301 aatgtaacat tgactggaat tccttcatca gtcaaagtcc tacaagcttt cagaccatct
     9361 ggagttagtg gacactttac aaccacattc tttccatatt ttcgtaatct acgtccttct
     9421 tcaatcatgc cagcagtgtc tgttgcaaca acttccaagc taacatcacc ttttactatt
     9481 ctaacaattt cttccattgt tttttgtggg tcaccacctt cttttgctaa aagtgatgga
     9541 tttgttgtaa taccatctac aagacccatg tcattgtata acttgattgc agcaatgttt
     9601 gcagtatcca agaatatttt catcgggatt tgctcctgat gtcttttact gaattagcta
     9661 tattaattga tgttatacca tgttttgcaa gcaatagtgg aatttcattg tctcgtgccg
     9721 actcaccaaa tttgtcctga gcaccaattc gtctcatctg tacaggatat gtttcggtga
     9781 ctacttc
//
LOCUS       DATIDL010000107         4278 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrosopumilaceae archaeon isolate SMAG_U16342
            k141_1345836, whole genome shotgun sequence.
ACCESSION   DATIDL010000107 DATIDL010000000
VERSION     DATIDL010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935836
            Sequence Read Archive: SRR25158326
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosopumilaceae archaeon (soil metagenome)
  ORGANISM  Nitrosopumilaceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrosopumilales;
            Nitrosopumilaceae.
REFERENCE   1  (bases 1 to 4278)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4278)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/04/2024 05:09:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,520
            CDSs (total)                 :: 1,474
            Genes (coding)               :: 1,474
            CDSs (with protein)          :: 1,474
            Genes (RNA)                  :: 46
            rRNAs                        :: 1 (5S)
            complete rRNAs               :: 1 (5S)
            tRNAs                        :: 43
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4278
                     /organism="Nitrosopumilaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1345836"
                     /isolate="SMAG_U16342"
                     /isolation_source="soil"
                     /db_xref="taxon:2202732"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..751)
                     /locus_tag="VLB45_04825"
     CDS             complement(<1..751)
                     /locus_tag="VLB45_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HSD05061.1"
                     /translation="MVVADLMLGTIVVPRTESPEAISRLTEFEWFHKIETQNDTVTPE
                     IDDLLLKAQKLYQSIDEVVKGLQIPARVGIMEVLFKGLVIKRNQYQLDEIASMISDLE
                     KKSGSIIDNPAKLLEDHANTKRSLEEYTSLRDTLEVVKKMNVNIGDLGQMKYFYTNLF
                     VINSSDYAEISRSLEGITIFKYDLDNKLKTAIIIFTDVYESERILKVLRSFNTNPFVI
                     PQGIPQNPSQAYGLVVSKIAELTAKEKKISKE"
     gene            851..1444
                     /locus_tag="VLB45_04830"
     CDS             851..1444
                     /locus_tag="VLB45_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010189546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033178 - proton-transporting two-sector
                     ATPase complex, catalytic domain [Evidence IEA];
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E family protein"
                     /protein_id="HSD05062.1"
                     /translation="MTSSPALEKTVDKILNQSENELLASLTQSLEDSKNTLSNSLSLL
                     EQQYDSILADAKKEADKLQKQIVGSSALEARNKQLVLVEESVEKVFAKAIAKLNLLVR
                     NEDYTKLMTQLLDESVKALGTSNVTVECNSKDKSVIQSILSKFSGATLSSNTINCIGG
                     IIVKSKDGSMSFDNTIDARIERLKPLIRKDIAVRFGR"
     gene            1447..3225
                     /locus_tag="VLB45_04835"
     CDS             1447..3225
                     /locus_tag="VLB45_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012216073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HSD05063.1"
                     /translation="MVAKGKIVWVSGPAVKADGMSDAKMYETVSVGNLKLIGEVIRLT
                     GDVAFIQVYESTSGLKPGEPVVGTGAPLSVLLGPGIIGQIYDGIQRPLNELSKKSGSF
                     IGRGVTTTPVDMTKKWKFTPTVKVGDQVQGGDVLGTVQETQLINHSILVPPDHPVSKV
                     TSIVGEGEYDLETEIAVTESDGKKIPLKMYHRWPVRKPRPYRQRFDPTVPLITGQRVI
                     DTFFPIAKGGTGSIPGAFGTGKTVTLHQIAKWADSQVVVYIGCGERGNEMTEVLVEFP
                     HLKDPTTGRPLMERTVLVANTSNMPVAAREASIYTGVTIAEYYRDMGKHVVLVADSTS
                     RWAEALREMSGRLEERPAEEGYPSYLASRLAEFYERAGRVSALGSPEREGSVTLVGAV
                     SPSGGDFTEPVTTHTIRFIKTFWALDARLAYSRHYPSINWMNSYSGYLSDIAKWWSAN
                     VSDEWLHLRNEAYSILQREDTLKEIVRLLGPEALPDEEKLILEVARMIKIGILQQNSF
                     DNVDTYCSPKKQLKLLKLMVNFYKGGQRALKEGATLPDIRAMSVIGNMLKARMEVPDD
                     QIAKLDQLDSLMNEQFKNIMGVKVAT"
     gene            3222..>4278
                     /locus_tag="VLB45_04840"
     CDS             3222..>4278
                     /locus_tag="VLB45_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007551535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HSD05064.1"
                     /translation="MTLEGGIEYSKIAEIKGPLVIVDGVDKASFDELVEIQTTSGEKR
                     LGKVMEVGFGKAIVQVFEGTTGLSISGTKAKFIGKTMTMPVSAEILGRVFDGLGRPND
                     GLPDPIAAKFLDINGEPMNPEQRDYPKDFIQTGVSVIDGMLTLVRGQKLPIFSGSGMS
                     HNILAAQIARQATVIGSGEEFAVVFAAIGVQYSEAQYFKRSLEESGALKRSVLFLNLA
                     DDPAIERIITPRVALTVAEYLAYDLGMHVLVILTDMTNYAEALREISAAREEVPGRKG
                     YPGYLYTDLSTIYERAGKLNGRKGSVTQVPILSMPSDDITHPIPDLTGYITEGQVVLG
                     RDLFRQGVYPPINILMSL"
BASE COUNT         1426 a          788 c          866 g         1198 t
ORIGIN      
        1 gttcttttga aattttcttt tctttagcag taagttcagc aatctttgaa acaaccaagc
       61 catatgcctg acttgggttc tgtggtatcc cttgtggaat tacaaacggg ttggtgttaa
      121 aacttcgaag aaccttgaga attctttccg attcgtatac atcggtaaaa attataattg
      181 cagtttttag tttgttgtcc aaatcatatt tgaaaattgt tatgccttca agagaacggc
      241 taatttctgc ataatctgaa gaatttatca caaacagatt tgtgtaaaaa tatttcatct
      301 ggcctaaatc gcctatgttt acattcattt tttttactac ctcaagtgtg tctctaagac
      361 tggtgtattc ctcaagagag cgttttgtgt ttgcatgatc ttctagaagc tttgctggat
      421 tatcaataat agagccactc tttttttcca agtcactgat cattgatgca atttcatcaa
      481 gttgatactg gtttcttttt attactagtc ccttgaacaa gacttccatt atgccaactc
      541 tggcaggaat ctgcaaacct tttacaactt catcaattga ctggtataat ttttgagcct
      601 ttaataacaa atcatcgatt tctggagtga ccgtatcgtt ttgtgtttct atcttatgaa
      661 accactcaaa ttctgtaagt cttgatattg cttctggaga ctctgtcctt ggtactacaa
      721 ttgttcccaa cattagatct gctactacca agacagactt tcggatgatg ccacgttttt
      781 aaaacgtttc taagaaattt ttttttgctt gacaaaacat tcaaatctgt tagcagtaaa
      841 cggctgatcg atgacatcta gccctgcact agagaaaacc gttgataaaa ttcttaatca
      901 atctgaaaat gaactcctcg caagcctaac acagtcactt gaggattcta aaaatacgct
      961 ttctaactcc ctttccttgc ttgaacagca gtacgacagc atacttgcgg atgccaaaaa
     1021 agaggcagac aaactccaaa aacaaatcgt aggcagctct gcacttgagg caagaaacaa
     1081 gcagttggtc ttagttgagg agtcagttga gaaggtcttt gcaaaagcca tagcaaaact
     1141 gaatcttttg gtacgaaacg aagactatac caaactcatg acacagctac ttgatgaatc
     1201 agtaaaagca cttggcacat ctaacgttac agtggaatgt aattcaaaag ataaatcagt
     1261 catacaatca attttgtcca agttttctgg agcaacacta tcttctaaca caataaattg
     1321 tattggtggt attatagtaa aatcaaaaga tggatcgatg agctttgata atacgatcga
     1381 tgcaagaatc gaacgtctaa agcctttaat aaggaaagac attgcagtca gattcggtag
     1441 atagctttgg tcgccaaagg aaaaattgtt tgggttagtg gacctgcagt caaagcggac
     1501 ggaatgtctg acgcaaaaat gtatgaaaca gtttctgttg gaaatctaaa actcattggt
     1561 gaagtaattc gtttaacagg tgatgttgca ttcatccaag tttatgaatc aacaagcggt
     1621 cttaaaccag gagaaccagt tgtcggtact ggtgctccac tgagtgtttt acttggtcct
     1681 ggaatcattg gacaaattta tgatggaata caaagaccac taaacgaact ttcaaaaaaa
     1741 tctggctctt ttattggcag aggagttaca acaactcctg ttgacatgac aaaaaaatgg
     1801 aagtttacac caactgttaa ggtaggtgat caagtacaag gaggagacgt acttggaaca
     1861 gtgcaggaaa ctcaactaat caaccacagt attcttgttc cacctgacca tcctgttagt
     1921 aaagtaacta gtattgttgg tgaaggtgaa tacgaccttg agacagagat agctgttaca
     1981 gaaagtgatg gaaagaaaat cccactaaag atgtatcata gatggccagt aagaaaaccg
     2041 cgaccatata gacagagatt tgatcctaca gttccattaa tcacaggaca gcgtgtcatt
     2101 gatacattct tcccaatagc aaagggagga acaggttcca ttcctggtgc atttggtact
     2161 ggaaaaactg taacattgca ccaaattgca aaatgggcag attcccaagt tgtcgtttac
     2221 attggatgtg gagaaagagg aaacgagatg accgaagtcc ttgtagaatt tccacatcta
     2281 aaagatccaa ctactggaag acccttgatg gaaagaactg ttcttgttgc aaacacaagt
     2341 aacatgccag tagctgcaag agaagcaagt atttacaccg gagttacaat tgctgaatac
     2401 tacagagaca tgggaaaaca tgtcgtactt gttgcagact cgacaagcag atgggcagaa
     2461 gctttgcgag aaatgagcgg aagactagaa gaaaggcctg cagaagaagg atatccatca
     2521 tatcttgcgt caagattagc agaattttat gaaagagcag gacgtgttag tgctcttggc
     2581 agtccagaaa gagaaggctc tgtaacactt gttggcgcgg tatccccatc tggaggtgac
     2641 tttacagaac ctgtaacaac tcacacaatt agattcatca aaactttctg ggcacttgat
     2701 gccagacttg catattccag acactatcca tcaattaact ggatgaatag ctattctgga
     2761 tatctatctg acattgcaaa gtggtggagt gcaaacgtta gtgatgaatg gctgcatctt
     2821 agaaatgaag catattctat actacaaaga gaagacaccc taaaagaaat cgtaagatta
     2881 ctaggacctg aagcactacc agatgaagaa aaactcatct tggaagtagc gagaatgata
     2941 aagataggaa ttttacaaca aaactcgttt gataatgtag atacctattg cagccccaaa
     3001 aaacaactca agcttctaaa actcatggtt aatttctaca agggaggcca acgtgcgcta
     3061 aaagaaggtg caactcttcc agacattcgt gccatgtcag taattggtaa tatgctaaaa
     3121 gcaagaatgg aagttcctga cgatcaaatt gcaaaacttg atcaacttga ttcattgatg
     3181 aatgaacaat tcaaaaacat catgggagtg aaagtcgcaa cttgacatta gagggcggaa
     3241 tagaatacag caaaattgca gaaattaaag gaccacttgt tattgttgat ggagtagaca
     3301 aggcttcatt tgatgagctt gtagaaattc aaacaactag cggagaaaaa agactaggaa
     3361 aagtcatgga agttggattt ggaaaagcta tagttcaggt ttttgaagga acaacaggcc
     3421 tttcaatttc tggaacaaaa gcaaaattca ttggaaaaac aatgaccatg ccagtttctg
     3481 ctgaaattct tggtagagta tttgatggac ttggtagacc aaatgacgga cttcctgatc
     3541 caatagctgc taaattttta gatatcaacg gtgaaccaat gaatccagaa caaagagatt
     3601 atccaaagga ctttatccaa actggcgttt ctgtgataga cggaatgctt actcttgtta
     3661 gaggtcagaa gcttccaata ttttctggtt ctggaatgtc acacaacatc ttagctgctc
     3721 aaattgctag acaagcaaca gtaattggaa gtggtgaaga atttgctgtg gtctttgcag
     3781 caattggagt gcaatacagc gaagcacaat atttcaagcg aagtcttgag gaatctggcg
     3841 cgctcaaaag aagcgtacta ttccttaatc ttgctgacga tcctgcaatt gaaagaatca
     3901 tcacacctcg tgtagcactt acagtagctg agtatctagc atatgacctt ggcatgcacg
     3961 tacttgtcat acttacagac atgacaaatt atgcagaagc actaagagaa atcagtgcag
     4021 caagagaaga agtacctgga agaaaaggct atccgggtta tctctatact gatctttcaa
     4081 caatttatga aagagcagga aaattaaatg gaagaaaagg aagtgttaca caagttccaa
     4141 ttttatcaat gccatctgat gacatcactc atccaatacc tgatcttact ggatacatta
     4201 cagaaggaca agttgtactt ggacgagatt tattcagaca aggtgtttat ccaccaatca
     4261 atattttgat gagtctta
//
LOCUS       DATIDL010000108         3509 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrosopumilaceae archaeon isolate SMAG_U16342
            k141_2762966, whole genome shotgun sequence.
ACCESSION   DATIDL010000108 DATIDL010000000
VERSION     DATIDL010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935836
            Sequence Read Archive: SRR25158326
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosopumilaceae archaeon (soil metagenome)
  ORGANISM  Nitrosopumilaceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrosopumilales;
            Nitrosopumilaceae.
REFERENCE   1  (bases 1 to 3509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/04/2024 05:09:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,520
            CDSs (total)                 :: 1,474
            Genes (coding)               :: 1,474
            CDSs (with protein)          :: 1,474
            Genes (RNA)                  :: 46
            rRNAs                        :: 1 (5S)
            complete rRNAs               :: 1 (5S)
            tRNAs                        :: 43
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3509
                     /organism="Nitrosopumilaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2762966"
                     /isolate="SMAG_U16342"
                     /isolation_source="soil"
                     /db_xref="taxon:2202732"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(54..782)
                     /locus_tag="VLB45_04845"
     CDS             complement(54..782)
                     /locus_tag="VLB45_04845"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HSD05065.1"
                     /translation="MKIVHVGAISTGIVILLGIVMVIPPFIQPSPPLKVAFAFSAIDE
                     NNALNWCRDLSQVLEKYDIKATVFVTGRIADLHPNCVRGFSDNIDIGSQTYHYVSLTT
                     INDYEAQLDEIKQGKEAVDRAGKLSSKVFRAPYGTTDENIYSLLRSSGILADFSYEYQ
                     YNKYYQGDFIKFDLNEYDGNEHDTDFFRSLPVTNPILITFDDSTPVEKIDDFISKLKS
                     RHIVFVNASELTGIELTIRQGVKN"
     gene            complement(794..1708)
                     /locus_tag="VLB45_04850"
     CDS             complement(794..1708)
                     /locus_tag="VLB45_04850"
                     /inference="COORDINATES: protein motif:HMM:NF013175.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkA"
                     /protein_id="HSD05066.1"
                     /translation="MLEFNEKRRMHAMIFEQIHQTKGKDKKILIVGLGQIGYSNAEYM
                     TSRGLNVDGYDISEKAVQRALHDKVIQNSATSFKGYDYYIICISTHRPENMFMPYLDG
                     LFEIAHRIAREGKTDALVGIDSTITRGTSNKVKQILGHRLHVVHVPHRFYINEKKEHG
                     VRQTRVLGGCDTCCAKEAKYFYKEMLDIPLHIVNSVDVAELCKIVENSHRFIEIAFAE
                     ELKMVCDYSDIDFDELRKAVNTKWNIKILEAKEGIGGHCLPKDSQMFLDFSNNLLVTS
                     LIRAAKMIDQQYRFHLVQKVIPEMPMAT"
     gene            complement(2172..2714)
                     /locus_tag="VLB45_04855"
     CDS             complement(2172..2714)
                     /locus_tag="VLB45_04855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05067.1"
                     /translation="MEVLERVPTTNRITLRLPNQVLEILKTEAEKKDLPLNALITKIL
                     YKNISFDLHVKAMPNITIPHDLFINFIDKIDDSFVEDIAKAGPTTVKKLFKILGINYD
                     LDQIIYNYFDMIGKYCEWYQFSHQAKHDKYRLVFNSTLGPKWTKFLAMYVRSILESLK
                     IRIDEMSINDGLVVFEFSHR"
     gene            3071..3391
                     /locus_tag="VLB45_04860"
     CDS             3071..3391
                     /locus_tag="VLB45_04860"
                     /inference="COORDINATES: protein motif:HMM:NF026297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HSD05068.1"
                     /translation="MVTCEMCAILEISSKMVKREFRANIEVLSRIATAFSQNSSMKKT
                     HLHLATRISWTSFMRYLEWLQSKNYMISKMDGKEEKYELTETGREMFGMLLQFHDHVR
                     FAKQ"
BASE COUNT         1075 a          605 c          553 g         1276 t
ORIGIN      
        1 accgcatttt tggttctgaa tatttttgtg aagataattt tcaagctcta atgtcaattt
       61 ttcactcctt ggcgaatagt taattcaatt ccagttagct cagaagcatt tacaaaaaca
      121 atatgacgag attttagctt cgaaataaaa tcgtcaatct tctctactgg tgtagaatca
      181 tcaaaagtaa tgagtattgg attagttact ggaagtgaac gaaagaaatc ggtatcatgt
      241 tcattaccat catattcatt aagatcaaat ttaatgaaat ctccttgata gtatttgttg
      301 tattgatatt catacgaaaa gtcggcaaga attcctgaac tacgcaaaag ggaataaata
      361 ttttcatcag ttgttccata aggggctcta aaaaccttag aagatagttt tcctgctcta
      421 tctactgctt ctttaccttg ttttatttca tcaagctgtg cctcataatc attaattgtt
      481 gtaagtgata cataatgata ggtctggctt ccaatatcga tattatctga gaaaccccta
      541 acacagtttg gatgtagatc agctattcta ccagtaacaa atactgtggc cttgatatcg
      601 tatttttcta aaacctgtga taaatccctg caccaattaa gcgcattatt ttcatctatt
      661 gcgctaaaag caaatgcaac ttttagtgga ggactaggct ggatgaaggg tggtattacc
      721 atcacaattc caagtagaat tactatacca gtagagattg caccgacatg gacaattttc
      781 ataaatttaa cctctatgtt gccataggca tttcaggtat tactttttga accaagtgga
      841 atctgtattg ctggtcaatc atttttgcag ctctgattag gctagtcact agcaagttat
      901 ttgagaaatc caaaaacatc tggctgtctt ttggcagaca gtgtcctcca atgccttctt
      961 ttgcttcaag aattttgata ttccatttag tatttactgc ctttcttaat tcgtcaaaat
     1021 caatgtcaga ataatcacat accatcttta gctcttcagc aaaagcaatc tcgatgaatc
     1081 tatgcgagtt ttcaactatt ttgcaaagct ctgcaacatc aacagaattt actatgtgca
     1141 aaggtatgtc tagcatctcc ttgtaaaaat acttggcttc ttttgcacaa catgtatcac
     1201 agcctccaag cactcttgtt tgtcttactc cgtgctcttt cttctcgttt atgtaaaatc
     1261 tgtgtggcac atgtactacg tgtagtctgt ggccaagaat ttgttttact ttattggatg
     1321 ttcctctggt aatcgtgctg tctattccca caagggcatc tgtttttccc tctcgtgcaa
     1381 ttctgtgagc tatttcaaag agtccatcaa gatatggcat aaacatattt tctggtctgt
     1441 gagttgaaat acaaattatg taatagtcat atcctttaaa gctggtagca ctattttgaa
     1501 ttactttgtc atgtaatgct ctttgtactg ctttttcact gatgtcataa ccatctacat
     1561 tcagccctct tgatgtcatg tattctgcat tgctataccc tatttgaccc aacccgacta
     1621 tcagaatttt tttgtctttt cctttggttt gatgtatttg ctcaaaaatc attgcatgca
     1681 ttctcctttt ttcattaaat tccaacaatt tccatgatac cacctgtcgc catagattgt
     1741 atcaccctca accagctcca tcgcctcatt gcacaatgaa catttttgaa caagaaaatt
     1801 ttgtgtcttc attgtagttc caattgagtc tgcacttaat tggaaatttt ttgatccatg
     1861 atttgattct accatgtttt tcatttcaat ctcatttcca aacatggtaa ataaaagacg
     1921 cgtagctttt ttactgatga tagagcagta atagttatat ttctaaaaaa actagttttg
     1981 tctaaaattc tggttttcaa caaaagtgga attttactat tcaaaattaa caataatttt
     2041 ttttctgtaa aaccatgtga tcctagatat ttgagaatgc gtgtcttatt tgtgctcatt
     2101 tttgatcgtt atttccattt ttggaaatga ctaataggca tttagaggca cactgtgtca
     2161 gtttgagtca attatctatg agagaactcg aagactacta gtccatcatt gattgacatc
     2221 tcatcaatac ggatttttag tgattcaagt atagatctca cgtacattgc aaggaatttt
     2281 gtccatttgg ggcctaatgt actgttaaat acaagtcggt atttgtcatg ttttgcttga
     2341 tgtgaaaatt gataccactc gcaatatttt ccaatcatgt caaaatagtt gtatattatt
     2401 tgatccaaat cataatttat gcccagaatt ttgaataatt tcttaactgt agtgggtcct
     2461 gccttggcaa tatcttctac aaacgaatca tcgattttat caataaaatt aatgaataaa
     2521 tcatgtggta ttgttatgtt tggcattgct ttgacatgca gatcaaatga tatgtttttg
     2581 taaaggatct tagttatcag cgcattcaga ggtagatctt ttttctctgc ttctgttttt
     2641 aatatttcca aaacttggtt tggcaaccgt aatgttattc tgttagtggt tggcactctt
     2701 tccaaaactt ccatatgata tcatctaaaa aatcgaagtc tgtttaatta taaaatattg
     2761 tgtatgtttg atactgtaca tacaaaaaat ctatttacaa tcatacgatg acattcgaat
     2821 cctgtatcat gtatctgaac tatgattgtg tcaagatgcg tcaggcggtg tcaacacaaa
     2881 atacgtatgg atttttcaga atctactcac acatttttac aatgtaatca aatgtatgat
     2941 atgaaattct cactacaatc ttatagacta tatgggaata caagaattat acaaattttt
     3001 taatctttga ttgataatta atgtaatagt agaaaccgta caaaacattt taaagcaaag
     3061 taagcgatag ttggttacat gtgagatgtg tgcaatactt gagatatcat ctaagatggt
     3121 aaagcgagaa tttagagcta atattgaagt tcttagccgt attgcaactg cattttcaca
     3181 aaactcttct atgaaaaaaa cacacttaca tcttgccact agaataagct ggacttcatt
     3241 tatgagatat ttggaatggt tacaatccaa aaattatatg atttctaaaa tggatggcaa
     3301 agaagagaaa tatgaactta cagaaactgg aagagaaatg tttggtatgc tcttacaatt
     3361 ccatgatcat gttagattcg ctaaacagta atttttatta tgtctcacca aaattaattt
     3421 tgtaaaataa aacaaaaatt catttatttg gtttcatatg gaaatttttt gttacaaatt
     3481 tttattcttt ttataagtat tgctcactt
//
LOCUS       DATIDL010000109         4208 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrosopumilaceae archaeon isolate SMAG_U16342
            k141_1146239, whole genome shotgun sequence.
ACCESSION   DATIDL010000109 DATIDL010000000
VERSION     DATIDL010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935836
            Sequence Read Archive: SRR25158326
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosopumilaceae archaeon (soil metagenome)
  ORGANISM  Nitrosopumilaceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrosopumilales;
            Nitrosopumilaceae.
REFERENCE   1  (bases 1 to 4208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/04/2024 05:09:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,520
            CDSs (total)                 :: 1,474
            Genes (coding)               :: 1,474
            CDSs (with protein)          :: 1,474
            Genes (RNA)                  :: 46
            rRNAs                        :: 1 (5S)
            complete rRNAs               :: 1 (5S)
            tRNAs                        :: 43
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4208
                     /organism="Nitrosopumilaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1146239"
                     /isolate="SMAG_U16342"
                     /isolation_source="soil"
                     /db_xref="taxon:2202732"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..267
                     /locus_tag="VLB45_04865"
     CDS             <1..267
                     /locus_tag="VLB45_04865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05069.1"
                     /translation="ELVSSVMKDLQLSCGVTDGFLLSEPIEKFGWSFFKILFKNELLV
                     GMQEKFGTQIMDTKGKAFQEKFTGFVTKFFESKNCKVRMKMLED"
     gene            complement(264..668)
                     /locus_tag="VLB45_04870"
     CDS             complement(264..668)
                     /locus_tag="VLB45_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020266891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HSD05070.1"
                     /translation="MLSEQEAKEKWGIAHIYSSYNNTIVHITDVSGAETVAISSGGRH
                     VNADRYESSPYAAMKSTGAVVEAAHDKGFTGLHIRVRAVGGVGARVPGPGAQAAIRAL
                     ARGGFKIGRIDDVTPIPHDTTRKKGGKRGRRV"
     gene            complement(699..1649)
                     /gene="thiL"
                     /locus_tag="VLB45_04875"
     CDS             complement(699..1649)
                     /gene="thiL"
                     /locus_tag="VLB45_04875"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HSD05071.1"
                     /translation="MSKLNEAEIIRIFQQRFGRKSKFVPEDVEIIRLDKTIFVTKSDM
                     LVESTDVPQGMKTNQIARKSMVACVSDFASKGVKPTYATISLAIPRSFSRSKISDLAN
                     GFMKASKEFGVKIIGGDVNEGKEIVIEVSMFGVTKKIVNRKGARLKDIIITSGPFGYA
                     SSGLKIILEHIKADYKFSKKCKNLVFVPKPRLGFGLSIAKYISSSMDSSDGLSTTLNE
                     MSKQSKKKFVITKLPSNPEVKKFADANRIRFTDLVFHGGEEYEIVATVSKNNLSKIKR
                     IAKIQKIPLFEIGYVTSGKNVIYQQKNKIIKDAGWLHFKS"
     gene            complement(1618..2931)
                     /locus_tag="VLB45_04880"
     CDS             complement(1618..2931)
                     /locus_tag="VLB45_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="HSD05072.1"
                     /translation="MKVSISGVRGIFGHDLNLNDVIKFCQNFSVLVKSKKCVVAQDTR
                     PSSKILSEVVLASLMERGIDVYNLGIAPTPVIFREARKYGSGVMITSSHNPLEWNGLK
                     FILEGRGINETELEQIKKENKLTKEKIGSESQIDSEYIQDAIKLIGPVEGSPRVAIDV
                     GGGAAFHVAPNLLHDIGCKVQTINEKPGISSRGPDPTADKLNDLISATKKCDIGFAFD
                     LDGDRLVVVKDGKKQTPDITLGLGIAKAIEIGYKRFVLSIDTSVSVEKFIKDHGGQVH
                     RSKVGEANVVDLMLKTNSQAGGEGSSAGFILPEFNMCRDGLLTSGLIAAMLGTSTFTD
                     VMKFMSGYLQLRTKVDVEAEFHEKTLQVLSEKMKEKYSDVITIDGIKSIIDEDSWTLV
                     RQSNTEHIVRISTESTSADKAKMIQKQVIELVNQSYEQAKRSRNN"
     gene            2984..3057
                     /locus_tag="VLB45_04885"
     tRNA            2984..3057
                     /locus_tag="VLB45_04885"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3018..3020,aa:Thr,seq:cgt)
     gene            complement(3086..3667)
                     /gene="dps"
                     /locus_tag="VLB45_04890"
     CDS             complement(3086..3667)
                     /gene="dps"
                     /locus_tag="VLB45_04890"
                     /EC_number="1.16.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA protection during starvation protein"
                     /protein_id="HSD05073.1"
                     /translation="MTEKIDVVGINVLKQNGANVDEITKLLVYNASVEFTAYYYFTLL
                     RAHCTGMEGEGIKGVIEDARLEDLSHFESCIERIHQLGGTLPIDATDFIKMSGCEFLQ
                     LPQNRTDLKSILEKCLKAEQGAIVNWNKMCNLTFGKDPTTYEIAKDILAEEIEHESWF
                     LELLHGRPSGHMRRKYSGERPHTRKHSRALDHL"
     gene            complement(3962..4132)
                     /locus_tag="VLB45_04895"
     CDS             complement(3962..4132)
                     /locus_tag="VLB45_04895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05074.1"
                     /translation="MRNKTLEIIGFVLIGVMIYAMFGGGENGPIWIIFWPCAIGLVGI
                     NVYRAKKKKQIK"
BASE COUNT         1157 a          865 c          677 g         1509 t
ORIGIN      
        1 gaacttgttt cttcagtaat gaaagatctt caattatctt gtggagtaac tgatggtttt
       61 ttgcttagtg agccaattga aaaatttggt tggagtttct ttaagatatt attcaagaat
      121 gaattacttg taggtatgca agaaaaattt ggcactcaga taatggacac aaagggaaaa
      181 gcatttcaag agaaatttac tggatttgtt acaaaatttt ttgaatctaa aaactgcaaa
      241 gtaagaatga aaatgcttga ggactagacc cttcttccac gtttgccgcc cttctttcgg
      301 gtcgtatcat gtggaatagg agtgacatca tcaattcttc ctatcttaaa tccgcctctt
      361 gctagtgctc taattgctgc ttgtgcacca gggccaggta ctctggcacc aactccgcca
      421 accgctctaa ctcgtatgtg aagtccagta aagcccttgt catgtgctgc ttctactact
      481 gcacctgtag atttcattgc tgcatatgga gacgattcgt atctgtctgc atttacatgt
      541 ctgccaccag agctaattgc aacagtctca gcacctgaga cgtctgttat gtgaacaatt
      601 gtgttgttat aactactata gatgtgggca attccccatt tttccttggc ttcttgttca
      661 gacaacattt tccaagctct gcttgggttt aaaaaacatt aggatttgaa atgaagccaa
      721 cccgcgtctt tgatgatttt gtttttctgc tgataaatga catttttgcc acttgtaaca
      781 tatcctatct caaataatgg aattttttga attttggcaa ttcgtttaat ctttgataaa
      841 ttgttctttg agactgtagc aacaatctca tattcctcac ctccatgaaa aactagatca
      901 gtaaatctga ttctatttgc atctgcaaat ttcttcactt ctggattgct aggaagtttt
      961 gttattacaa atttcttttt gctttgcttg ctcatttcat ttagtgttgt agacaatcca
     1021 tcgcttgaat ccattgaaga tgaaatgtat tttgcaatgg acaaaccaaa acccaatctt
     1081 ggttttggga caaaaactag atttttacat ttttttgaaa acttgtaatc tgctttaata
     1141 tgttctaaaa taatttttag gcccgaagaa gcatatccaa atgggcctga tgttatgatt
     1201 atgtctttta atctagctcc tttcctgtta actatttttt ttgtaactcc aaacattgat
     1261 acttcgatga caatttcttt tccctcattt acgtctccac ctattatttt aacgccaaat
     1321 tcttttgatg ctttcataaa tccattagct agatcgctta tcttggatct ggaaaaactc
     1381 cttggaattg caagcgagat agtggcatag gtaggcttta ctccttttga cgcaaaatcg
     1441 cttacgcagg caaccatgct ctttcttgca atttgattag tcttcatacc ttgtggaaca
     1501 tcggtacttt caactagcat gtctgatttg gtaacaaaga tagttttgtc cagtctgatg
     1561 atctcaacat cttcaggtac aaactttgat tttctcccaa atcgctgttg aaatattcta
     1621 attatttctg cttcgtttag cttgctcata actctgattt actaattcta tgacctgctt
     1681 ttgaatcatt tttgccttgt ctgcactggt tgattctgtt gatattctca caatatgttc
     1741 agtatttgat tgccgtacga gtgtccagct gtcttcatct atgattgatt ttataccatc
     1801 gattgttatc acatcactgt acttctcttt cattttttca gatagaacct gtaatgtttt
     1861 ttcatgaaat tcagcttcta catccacctt tgttcttaac tgcaaatatc cactcatgaa
     1921 tttcatgaca tcagtaaaag tactagtccc aagcatggca gctatcaatc cgctagttaa
     1981 tagaccgtcc cgacacatgt tgaattctgg cagaataaat cctgcactac ttccttctcc
     2041 gcctgcctga gaatttgtct ttaacattaa atctacaaca tttgcttcgc ccactttgga
     2101 tctgtgtacc tgaccaccat ggtctttgat gaatttctca actgatacac ttgtatctat
     2161 gctaagaaca aaccttttgt atcctatctc gatggctttt gcaattccaa gaccaagtgt
     2221 aatatccggt gtttgttttt taccatcctt tacaacaact agtctgtctc catctaaatc
     2281 aaaagcaaat ccgatgtcac atttttttgt ggctgaaatt aagtcattta atttatctgc
     2341 agtaggatct gggcctcgtg aagaaattcc aggcttttca tttattgttt gcactttgca
     2401 acctatgtcg tgaagcaagt taggtgcaac atgaaaggct gcccctccac caacatctat
     2461 ggctactctt ggagatcctt ctacaggacc aattaatttt attgcatcct gtatatactc
     2521 tgaatcaatt tgtgattcag aaccaatctt ctcttttgtt agtttatttt cttttttgat
     2581 ctgttctaat tcagtttcat ttattcctct gccttctagt atgaacttca gaccgttcca
     2641 ctccaaagga ttgtgtgacg atgtgatcat cacaccgcta ccatattttc ttgcctctct
     2701 aaatatgaca ggagttggtg ctattcccaa gttgtataca tcaatacctc tttccatcag
     2761 agatgcaagc actacttcac tgagaatttt acttgatggt cttgtgtctt gagctacaac
     2821 acatttttta gatttcacta gtacagaaaa attctgacaa aattttatta catcattgag
     2881 gttgagatcg tgaccaaaga ttcctctcac accggagatg gatactttca atgaatcaaa
     2941 agcacaaagg ctttttataa actctgatga caaagcgaac cgtgcctcga tagctcagcc
     3001 tggtagagcg aacgcctcgt aagcgttagg ccgcgggatc gaatcccgct cgaggctttt
     3061 tttgaaatga aatttaattt tcagtctaga gatgatctaa tgctctagaa tgcttgcgtg
     3121 tgtgaggtct ttcaccagaa tattttcttc gcatatgtcc agacggtctg ccgtgtaaaa
     3181 gctctaggaa ccatgattcg tgctcaattt cttctgcaag aatgtctttt gcgatctcat
     3241 atgtggttgg atctttacca aaagtcaaat tgcacatctt attccagttt acgattgctc
     3301 cttgttcagc tttcagacat ttttctagaa ttgacttcaa atcagttcta ttttgtggca
     3361 gttgcaaaaa ttcacaacct gacattttga tgaaatctgt ggcatcgatt ggaagtgttc
     3421 cgccaagttg atgaattctt tctatacatg attcaaaatg actaagatct tctagtcttg
     3481 catcttcgat cactcctttg atgccttcac cttccatgcc agtacagtgt gctctaagga
     3541 gtgtaaagta atagtatgct gtgaattcaa ctgatgcatt atagactagc agctttgtga
     3601 tttcgtctac atttgcacca ttttgcttca aaacattgat tccaactaca tcgatttttt
     3661 cagtcattct gatactctaa aatcggatat gctacgaaat ttaaagatat ctatattaaa
     3721 ttaaaattca aaatttactt gcttgcatta ttttcttctt gactttcaag atcaacttca
     3781 agttccttac ctaaaccgtt ccaccatgct aaaatctctc tcatatttct cccgaaactt
     3841 acgaaggaat cctcctttat agatctgtcg gtttttttct ttttgccaga tttttataca
     3901 aaaacaaaac tttttgtttt aacaaccgat tcaataccaa aaaaacgcaa tcttgaaatc
     3961 tttattttat ttgttttttc ttttttgcac gatatacatt aattccaacg agccctattg
     4021 cacatggcca aaaaataatc cagattggac cattttctcc accaccaaac atggcataaa
     4081 tcattacacc tatgaggaca aatccaatta tctctaatgt tttgttacgc atgtttgaat
     4141 acaagacaga tagaatcaaa aggatatatt gttttgagat ttatcatctg tatggacaaa
     4201 tatctagt
//
LOCUS       DATIDL010000110        10600 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrosopumilaceae archaeon isolate SMAG_U16342
            k141_1955149, whole genome shotgun sequence.
ACCESSION   DATIDL010000110 DATIDL010000000
VERSION     DATIDL010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935836
            Sequence Read Archive: SRR25158326
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosopumilaceae archaeon (soil metagenome)
  ORGANISM  Nitrosopumilaceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrosopumilales;
            Nitrosopumilaceae.
REFERENCE   1  (bases 1 to 10600)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10600)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/04/2024 05:09:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,520
            CDSs (total)                 :: 1,474
            Genes (coding)               :: 1,474
            CDSs (with protein)          :: 1,474
            Genes (RNA)                  :: 46
            rRNAs                        :: 1 (5S)
            complete rRNAs               :: 1 (5S)
            tRNAs                        :: 43
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10600
                     /organism="Nitrosopumilaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1955149"
                     /isolate="SMAG_U16342"
                     /isolation_source="soil"
                     /db_xref="taxon:2202732"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            231..1397
                     /locus_tag="VLB45_04900"
     CDS             231..1397
                     /locus_tag="VLB45_04900"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF003338.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HSD05075.1"
                     /translation="MKLQVDILGIESGGKPIVFLNKLDADEIGVTASGRVKIKATKEI
                     TAIVNVATTSMKKGFIGINEEIRKTLSINQNSVVDVEVSSFPKSLQFIRNKLSGKKLL
                     YDEIYEIVKDVVAGNLNEGEIASFVTALHIQGLGLDEATSLASSMVKTGKQLKLDAKI
                     IVDKHSIGGVPGDKTTLLVVPIVAAAGLTIPKTSSRAITSAAGTADRAEVLMPVNLGS
                     EEMQKVVKKCNGCIVWGGALELAPADDIFVKTEYSLYIDPLLLPSIMSKKKAVGATHL
                     VVDIPAGRGAKVKTIGEADLLAKDIIELGRRLNIHTHCVVTHGEQPIGYTMGPVLEAK
                     EALEVLMNKTNVPDLVDKACHIAGAIFEITGKKNGYNLAKEILRTGKAEKNSEK"
     gene            1457..1771
                     /locus_tag="VLB45_04905"
     CDS             1457..1771
                     /locus_tag="VLB45_04905"
                     /inference="COORDINATES: protein motif:HMM:NF019448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05076.1"
                     /translation="MLQVKAKKSGQVLWMDNNALVEIARAAGAPKDKGAGIIFNKKHG
                     DTVSKNDLLFTIYSEKTRKLNRSHDILREQEPIGIGKQMEMFIHKVKESPVIKRAFVL
                     DR"
     gene            1847..2350
                     /locus_tag="VLB45_04910"
     CDS             1847..2350
                     /locus_tag="VLB45_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007550277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05077.1"
                     /translation="MVNQYIWVGISAGLFFVGLGVGYSVFTNTYNPAYMMQNPQLMQK
                     MMQDPEFHQQMMNSIVQNPQMMNQWMGSMMQNQQFMQGMHSAMIQNSGHMQWMGNMMG
                     SNMASYMMNDPNLRQQMFSNMLEHHEFMEDLMRNQQFQQNWMGPWMMNNTNWQNMMGS
                     DMMQNNP"
     gene            complement(2378..3607)
                     /locus_tag="VLB45_04915"
     CDS             complement(2378..3607)
                     /locus_tag="VLB45_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010189953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00300 family protein"
                     /protein_id="HSD05078.1"
                     /translation="MTHRFEHEIEVHGHLIDSMILTKIFDVIMDLGGEFQVQEFKVGK
                     RKKDESYAKLLIQGKSQKHLDEILESVYREGATSKIQQKITLKQAPKDMVMPDNFYST
                     TNNRTDVFHKGKWIKVENMMMDKCIVVKGQRAWCSPIREIKKGDMIIVGESGIRITPP
                     ERPREGVNLFQFMGSNSSSERPTQHIARKVAHDIYKTKKEKGKIVLVGGPAIVHTGAS
                     DSIATLIRLGFIDGVLAGNALAVHDIEYSTLGTSLGMNVQNGTLAVRGHRNHMQAINS
                     VFKAGSISKMVSNGTLKKGIMYECVKRKIPFVLAGSLRDDGPLPDVMTDMIAAQKKYK
                     EILKNAKMVIMISTMLHSIATGNMLPAEVKVIVVDINQPTVTKLMDRGTWQALGIVSD
                     VGAFLPLVTQEIQKLIK"
     gene            complement(3717..3986)
                     /locus_tag="VLB45_04920"
     CDS             complement(3717..3986)
                     /locus_tag="VLB45_04920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05079.1"
                     /translation="MISTNAVFVAVLVCIAIIILAFVAFPFWNLIPSMVTETGTIVFK
                     GESGCVVETTDHYLVSVDSCDGNVGDTTHVTFDSKIKQRARAFLP"
     gene            4099..4680
                     /locus_tag="VLB45_04925"
     CDS             4099..4680
                     /locus_tag="VLB45_04925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05080.1"
                     /translation="MKLHFNKNHGNIRLSISVLLSVFSLSLFFQLVYGSDVNPAESYV
                     TIPDLAQLSGQKYLLTVGDNTYTIFYGMSPIDETDTSGSIQSISIVPENYSLLIQLDN
                     VSQTEVLWIRFPNEVISAENNEFTLFIDGEEKGYELSSHGDETRLSFLISNDAKKIEI
                     VGNNVIPEFPTVLLTLSMIFGMAVILSRYSLKR"
     gene            complement(4685..5032)
                     /locus_tag="VLB45_04930"
     CDS             complement(4685..5032)
                     /locus_tag="VLB45_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05081.1"
                     /translation="MQYFMALKIGEKRVKEAREYLNKYANGAAMPALALKDNKTNVWE
                     PVGEENLYAVLNDAGGYVLTDVSGYMIVLCDKNGISKAIARGLSTERKDSMIKMLQVD
                     NIQEYKGDVSLPV"
     gene            complement(5054..5584)
                     /locus_tag="VLB45_04935"
     CDS             complement(5054..5584)
                     /locus_tag="VLB45_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007550543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HSD05082.1"
                     /translation="MLFDFFNIITELKKTPRKGWKDKAGIESPESVADHSFNMAIMAM
                     VLSDLDGMGTEKILKMALLHDVAESITGDLTPDEISKEDKTKLEKQTMQEILTNLPSS
                     LSEKYVAIWDEYVNGYSKEAVLVHDIDRLEMALQARKYLAEGKPLDKLDVFFSSARKD
                     IKSEKILKLLDEISYK"
     gene            complement(5585..5707)
                     /locus_tag="VLB45_04940"
     CDS             complement(5585..5707)
                     /locus_tag="VLB45_04940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05083.1"
                     /translation="MTELVCPYCESKFVDKKDLSKHIDRLHGGAGLLEGDTRKF"
     gene            5782..6336
                     /locus_tag="VLB45_04945"
     CDS             5782..6336
                     /locus_tag="VLB45_04945"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HSD05084.1"
                     /translation="MVKIYTKTGDDGSTGLIGGKRVSKSNPRIIAYGAIDEVNSALGI
                     ALSSLHDKDIHELLEKIQNDLFVLGADLANDNLQNNTNRITENMIKFLENHIDKLESE
                     LSQITYFILPGGDLVASQIHMARAISRRAETQIISLAENEQINKLCQIYINRLSDLLF
                     VIARVINKRKMISDIAWKNRTDTL"
     gene            6364..6437
                     /locus_tag="VLB45_04950"
     tRNA            6364..6437
                     /locus_tag="VLB45_04950"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6398..6400,aa:Phe,seq:gaa)
     gene            6538..6765
                     /locus_tag="VLB45_04955"
     CDS             6538..6765
                     /locus_tag="VLB45_04955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05085.1"
                     /translation="MAKDKSKDKKKGKKKPAEKTTKKVAKTKPKLKQDLVAGDDAGII
                     VVEDDPNMDKDAEMEARRAYLEEARSQEAED"
     gene            6836..7075
                     /locus_tag="VLB45_04960"
     CDS             6836..7075
                     /locus_tag="VLB45_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05086.1"
                     /translation="MRALCLIAVKPGKVDNVIQSLKKARKIIREIMIVTGRADIAVVL
                     QGTIDEINNIVIDFKKIREIVTTETLIEVEVNMGW"
     gene            7069..7287
                     /locus_tag="VLB45_04965"
     CDS             7069..7287
                     /locus_tag="VLB45_04965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007401596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HSD05087.1"
                     /translation="MVKAVVLVKSPKKLIAARLRMVKSVYDSFAVSGQFDAVALIDVK
                     SLSQIKDVTTEIQNINGVERTETMVQVV"
     gene            7326..7736
                     /locus_tag="VLB45_04970"
     CDS             7326..7736
                     /locus_tag="VLB45_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HSD05088.1"
                     /translation="MNIRHLGSVIIAVSDLERSVKFYNEIIGMPIKNKRENWVELAKQ
                     GATVILHPASRPINTGTSIENGIVIGLVVGDIESAIQELKSQKVTVYREVTSHKVGKN
                     AIVLDPDKYMISLFEPALPDSAKQASGFHGFAPL"
     gene            complement(7733..8125)
                     /locus_tag="VLB45_04975"
     CDS             complement(7733..8125)
                     /locus_tag="VLB45_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05089.1"
                     /translation="MIKTQDQKWKDEIIAYRKKCQKILLISKSIRYVGLINEYGRTLT
                     GIIKQGTNPMLKAGQVRNEFFLISTMLSMRSTNYSAIGDMDYAVFKHKKVILVVFQRK
                     EGIYYISVDQKVSPDALTKIISNIKKII"
     gene            complement(8162..8533)
                     /locus_tag="VLB45_04980"
     CDS             complement(8162..8533)
                     /locus_tag="VLB45_04980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05090.1"
                     /translation="MWYHDPKKILEVYGIDITMMNSPLAFPRLPQMSNHPSGISKTGM
                     LKEKIIRDNASMLARLSGFHTAFQQYASNLFDLLPSSSQFGPGHPMHSMNSMNALESE
                     NEQLKKENATLKQNLEKTKKK"
     gene            complement(8824..9618)
                     /locus_tag="VLB45_04985"
     CDS             complement(8824..9618)
                     /locus_tag="VLB45_04985"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007550284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HSD05091.1"
                     /translation="MSEETPQEPPKPMKTKDIVDMMMGGNRSEIVDTETLIKEVQKRV
                     WSSLKKGYEYDYSRDDSDKFLRQHVSSRVKMIVLYVDLVGSTQLTLELPAEKLAIIIS
                     SFSQEMGFVIKHHEGHVLKYVGDAVIGYFVAVDNPLVAADNAVNCGKSMISVIERGIN
                     PILNQYDYPDLKVKIGMDFGENTIVRYGADEIKSHVDILGPGMSIAAKITSLAKPNQI
                     LVGEDVYEKLHPSLKQDFNEVKGNEGHWDYRDRETGKVYRVFAYIG"
     gene            9881..>10600
                     /locus_tag="VLB45_04990"
     CDS             9881..>10600
                     /locus_tag="VLB45_04990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05092.1"
                     /translation="MTEESEKVRSAFLEKITEHTKNVPIKVMLGDSTIVDQTTFDPAL
                     VRHYYQEILKKISDWKTQGISVVNDKDLRRIFLKFEIQEGNYLLSCHMSLQYHALLYY
                     KPNHRVIEIQKELSEIVDKTKNLETQIADQGNKIIQEKLKEIGQENADQQKIFEIFFQ
                     NDNLANTLVDKIYSSADENFEQLSKRKSDLFKELDNMLMEIYHTSTTLIDEMRMISAE
                     EGCLCHFDLEHLKKDGKEGTFD"
BASE COUNT         3484 a         1863 c         1859 g         3394 t
ORIGIN      
        1 tagattacat cgattacaaa atctggttca aaatattctg tatagaaact agccctcaaa
       61 accactgaat ttattcatac acatgtcatg catttgattc accattcatg catgaatatg
      121 agacagaaca taaaatataa catcatgata ttcctacaca tgataatcaa atatgataat
      181 tgcactgtcc tactaaaagt gagaaaattt gtaatatgag atcgttttag atgaaactac
      241 aagtagacat tttgggaatc gaatcaggtg gaaaaccaat cgtatttcta aacaaacttg
      301 atgcagatga aattggtgtc actgcatcag gcagagtgaa aataaaggca acgaaggaaa
      361 tcacagcaat agtaaacgtt gctactactt caatgaaaaa aggtttcatt ggaataaatg
      421 aagaaatacg aaaaacacta tcaataaatc aaaattcggt tgttgatgta gaagtatctt
      481 cttttccaaa atcattacaa ttcatacgca ataaattatc aggtaaaaaa ttactttatg
      541 atgaaattta tgagatcgta aaagatgtag tagcaggaaa tcttaacgag ggagaaatcg
      601 catcatttgt tactgcgtta cacatacaag gcttgggcct tgatgaagcc acaagtttag
      661 cttcttccat ggttaaaact ggaaaacaat tgaaacttga tgccaaaatt atagttgata
      721 agcatagtat tggtggggtt cctggagata agaccacatt acttgttgta cccatagtag
      781 ctgctgcagg actgacaatt ccaaagacat catctagagc aataacatct gcggctggaa
      841 ctgcagacag agcagaagtg ttaatgcctg tcaatctcgg aagtgaagaa atgcaaaaag
      901 tcgtcaaaaa atgtaatggt tgtatagtat ggggcggtgc tcttgaatta gcaccagctg
      961 atgatatttt tgtcaaaaca gaatattcgt tatacataga tccactactt ctgccttcga
     1021 ttatgagtaa aaagaaagca gtgggagcaa ctcacctcgt tgttgatatc cctgcaggaa
     1081 gaggtgctaa agtaaaaacc ataggtgaag ctgatcttct tgccaaagat ataatcgagc
     1141 ttggaagaag attgaacata catacacatt gtgtagtaac acatggtgaa caacctattg
     1201 gttatactat gggccctgta cttgaagcaa aagaagccct tgaggttttg atgaacaaaa
     1261 ccaatgtgcc agatcttgta gacaaggcat gtcatattgc aggtgcaatt tttgaaatca
     1321 ctggaaaaaa gaatggttac aatcttgcaa aagaaatact acgtacggga aaagcagaaa
     1381 aaaactcaga gaaataattt ctcttcaagg tggaaactct gacctgaagc ctaaagatct
     1441 agtgattgga gaacatttgt tacaggttaa agcaaaaaaa tctggacagg ttctttggat
     1501 ggataataat gcattagtag aaattgctag agctgctggt gccccaaaag ataaaggtgc
     1561 aggaataatc tttaataaaa aacacggaga tactgtatca aaaaatgatc ttctttttac
     1621 aatatattct gaaaaaactc gtaagcttaa ccgttctcat gatatcctta gagagcaaga
     1681 accaataggt attggtaaac aaatggagat gttcatacat aaagtcaaag aatcccctgt
     1741 catcaaacgt gcatttgttt tagacagata attgatcttt tcaacattga ttctcattga
     1801 tgatattttt cattgtctat aattattcaa ctttctaact ttacgtatgg ttaatcaata
     1861 catatgggtc ggcatttccg cagggttatt ttttgtcggt cttggagtgg gttatagtgt
     1921 ttttacgaat acctataatc ctgcatatat gatgcaaaat cctcaattga tgcaaaaaat
     1981 gatgcaggat ccagaatttc atcaacaaat gatgaacagc atagtacaaa atcctcagat
     2041 gatgaatcag tggatgggat caatgatgca gaatcagcaa ttcatgcaag gcatgcatag
     2101 tgcaatgata caaaattcag gccacatgca atggatggga aacatgatgg gctcaaacat
     2161 ggccagctac atgatgaatg atccaaatct aagacaacaa atgtttagca atatgttgga
     2221 acaccatgaa tttatggaag atttgatgag aaatcagcaa ttccaacaaa actggatggg
     2281 tccttggatg atgaacaata ccaattggca gaacatgatg ggttctgata tgatgcaaaa
     2341 taacccgtag tctttctcaa acttttattt tattacgtca cttgatgagt ttttgaattt
     2401 cttgtgttac tagaggcaaa aatgctccaa catcagagac tatgcccaac gcttgccatg
     2461 ttcctctatc cattaatttt gtaactgttg gctgattgat atccactaca ataactttga
     2521 cttctgccgg aagcatgttg cctgtagcta ttgaatggag catagtagaa atcataatta
     2581 ccatctttgc gttttttaga atctctttgt attttttttg agctgctatc atatctgtca
     2641 tgacatcagg caatggtcca tcatctctta atgaaccagc taaaacaaac ggaatttttc
     2701 tttttacaca ctcgtacatt attccttttt tgagcgtgcc atttgagacc atttttgata
     2761 tggaaccagc cttgaataca gaattgattg cctgcatatg atttcggtgc ccccttactg
     2821 ccaatgtccc attttgaaca ttcattccca aagatgtacc aagagtggaa tattctatat
     2881 catgaacagc caaggcattt ccagctaaca ctccgtcaat aaatccaagt cttatcagag
     2941 tagctataga atctgacgca ccagtatgca ctatggcagg acctcctact agaacaatct
     3001 tgcctttttc tttttttgtc ttgtaaatat catgtgcaac tttccgtgca atgtgttgtg
     3061 tcggtcgttc acttgagctg ttactgccca taaattgaaa aagattcaca ccttctcttg
     3121 gcctttctgg aggtgtaatt cttatcccac tttctcctac tatgatcatg tctccttttt
     3181 tgatttcacg tattggtgag caccacgcac gttgtccttt gactacaatg catttgtcca
     3241 tcatcatgtt ttcaacttta atccatttgc ccttgtggaa aacatctgta cgattatttg
     3301 ttgtactgta aaaattatct ggcatgacca tgtcttttgg tgcttgtttt agtgtaattt
     3361 tttgttgaat ctttgaagta gcaccttcgc gatatactga ttcaagaatt tcatcaagat
     3421 gtttttgtga ttttccttgt attagcagct tggcataact ttcatctttt tttcttttac
     3481 ctactttgaa ttcttggact tgaaattctc cacctagatc cattatgaca tcaaaaatct
     3541 ttgtaagtat catcgaatct atgagatgac catgtacttc gatttcatgc tcaaatctat
     3601 gtgtcatgcc tagaatggtg taattgtact aatttaatgt aatatcaagt cgaaaaacaa
     3661 ccggattttt gtctagatat tttatgtata tgagaaaaat cgaataaaag gtggatttac
     3721 ggcaagaatg ctcttgcacg ttgtttaatt tttgaatcaa atgtcacatg tgttgtatcg
     3781 cctacattcc catcacacga gtcaaccgat acaaggtagt gatctgttgt ttctacaaca
     3841 catcctgatt cccctttgaa aactattgta ccagtttctg ttaccatgct tggaatcaaa
     3901 ttccaaaacg ggaaggcaac aaatgccaat atgattatgg cgatgcagac tagtactgcg
     3961 acaaacactg catttgtact tatcatgacg gattttactg gaattggtat atgtattcaa
     4021 ttgacaatta ttgtcggtga aaatcatatc tgataactag aatttcattt aattgtacaa
     4081 aatttaaact aaaaatttat gaaattacat tttaacaaaa accatggtaa tattagatta
     4141 tccatttctg ttttattatc agtattttct ctttcattat tttttcaact agtctatgga
     4201 agtgatgtaa atccagctga atcttatgtg acaatcccgg atcttgccca attatcagga
     4261 caaaagtatt tgttaacagt gggtgataac acttacacaa ttttttatgg tatgtctccg
     4321 attgatgaaa ctgacacctc tggttcaatt caatcaatat ctattgttcc tgaaaattat
     4381 tctttgttga tacaattaga caacgtatca caaacagaag tattatggat acgattccca
     4441 aatgaagtga tttctgcaga aaataatgaa tttacacttt ttattgatgg tgaagaaaaa
     4501 ggatacgaat tatcctctca tggcgatgaa acacgactta gtttcttaat atctaatgat
     4561 gcaaagaaaa ttgagatagt aggaaataat gtgattcccg agtttccaac agttctgttg
     4621 acgctctcta tgatttttgg catggcagta attttatcta gatatagtct taaacggtaa
     4681 acccttacac tggaagagaa acatctcctt tgtattcttg aatattgtct acttgtagca
     4741 tctttatcat ggagtctttt cgctccgtgc ttaatcctct tgcgatggct ttggaaattc
     4801 catttttatc gcaaagtaca atcatgtatc cgctaacatc tgttagtaca tatccgcctg
     4861 catcattaag tactgcatag agattttctt caccaactgg ttcccagaca tttgttttgt
     4921 tgtctttgag tgcaagagct ggcatggcag caccgttagc atatttgttc agatactctc
     4981 tggcttcttt tactcgtttt tctcctattt tgagcgccat aaagtactgc atattgtatt
     5041 gaatgagttt ttgttattta taggatatct catctaagag tttcaaaatt ttttcacttt
     5101 tgatgtcttt tcgagcacta gaaaaaaata catcaagctt gtctaacggt tttccctctg
     5161 caaggtattt tcttgcctgc agcgccattt ccaaacgatc aatatcatgt accaataccg
     5221 cctcttttga atacccatta acatattcgt cccaaattgc aacatatttt tctgaaagac
     5281 ttgagggcag atttgttaaa atttcttgca tggtttgttt ttctaatttt gttttatctt
     5341 cttttgaaat ctcatctgga gtcaagtctc cagtgataga ttctgccacg tcatggagta
     5401 acgccatttt tagtattttt tccgtaccca taccatctaa atccgataag accattgcca
     5461 taattgccat gttgaaagaa tgatctgcta cggattcagg tgattctatg cccgccttat
     5521 ctttccaacc ttttcgtggt gtctttttta gttctgttat gatattgaaa aaatcaaata
     5581 gcaattagaa cttccttgta tcgccttcta gcagtcctgc tccgccatgt aatctatcaa
     5641 tgtgctttga taggtctttt ttatcgacaa attttgattc gcaatatgga cacactaact
     5701 ctgtcatgct ctaatcgccc atggatacca ctattaaata attcgaacaa gataaaaacg
     5761 ccaaaactat caaatcatgt attggtaaaa atctatacaa agacagggga tgacggctca
     5821 actggtctta tagggggaaa gcgggtatca aaatccaatc cacgaataat tgcatatggt
     5881 gcaatagatg aagtaaactc tgctttagga atagcacttt cgtcactgca tgataaagac
     5941 atccatgaat tgctagaaaa aattcaaaac gatctatttg ttttaggtgc agatcttgcc
     6001 aatgacaatc tacaaaacaa cacaaaccga atcactgaaa acatgataaa atttcttgaa
     6061 aaccacattg acaaactgga aagtgaattg tctcaaataa catattttat tttaccaggt
     6121 ggtgatttag tagcctctca aattcacatg gcaagagcaa tatcacgaag ggcagagact
     6181 caaatcatca gtcttgcaga aaatgagcaa ataaacaagc tttgtcagat ttacataaat
     6241 cgattatctg atctactttt tgtcattgca cgtgtaataa acaaaagaaa gatgatcagt
     6301 gacattgcat ggaaaaacag gacagacaca ctttaattcc ccacttattg gatattccga
     6361 tgtgccaccg tagctcagcc cgggagagca ctcggctgaa gaccgagctg tcgcgcgttc
     6421 aagtcccgcc ggtggcacta caatttgtct gtaattttag cttcacatag atcaatatag
     6481 tcaaattttg agacaagttt aataacacgg cttacaattt tgaaaaaagt cgtcatcatg
     6541 gcaaaagata aatcaaaaga taagaaaaaa ggcaagaaaa aaccagctga aaaaacaaca
     6601 aaaaaagttg caaaaactaa accaaagtta aagcaagatt tggttgcagg tgacgatgca
     6661 ggaattattg ttgtagaaga tgatcctaac atggataagg atgcagagat ggaagcaaga
     6721 agagcttatc ttgaagaagc acgttcacaa gaagccgaag attaaatcat aaactattca
     6781 agatggtttt tagatttcta taaacactta tccatacgca agttgtatta tcaatatgag
     6841 ggcactttgt ctaatagcag ttaaacctgg aaaagtagac aacgtaattc aaagcctcaa
     6901 aaaagcgcgt aaaattattc gtgaaataat gatagtgaca ggtagagcag acattgcagt
     6961 tgttttacaa ggaacaattg acgaaattaa caatattgta attgatttca aaaaaataag
     7021 agagattgtt acaacagaaa ctttgataga agttgaggtg aatatgggat ggtaaaagca
     7081 gtagttttgg taaagtcgcc aaaaaaacta attgctgcaa gattgagaat ggtaaaatca
     7141 gtttacgatt catttgcagt aagtgggcag tttgatgctg tagcactaat cgatgtaaaa
     7201 tcattatcgc aaattaaaga tgtaacaacc gaaatccaga acataaatgg cgtggaacga
     7261 accgaaacga tggttcaggt agtatagtag gaaagatttt aacactagag gtatgaaatc
     7321 ttgatatgaa cataagacat cttggcagcg tgataatcgc agtatcggat ttagaaagat
     7381 cggtaaaatt ctataatgaa atcataggaa tgccaataaa aaataaaaga gaaaactggg
     7441 ttgagcttgc aaaacaaggt gctacagtaa tcttgcatcc tgcaagtaga ccaattaata
     7501 caggaacttc aattgagaac ggcatagtaa ttggtttggt agttggagac atagaatctg
     7561 ccattcaaga actcaagtca caaaaagtta ctgtatatcg tgaggttaca tcacataaag
     7621 taggcaaaaa tgccatagtt ttagatcctg ataaatacat gatatcattg tttgagccgg
     7681 ccttacctga tagtgcaaaa caagcatcag gattccacgg ttttgcacca ctttagatta
     7741 tttttttaat attactgatt atttttgtta gagcatcagg tgatactttt tgatcaactg
     7801 aaatgtaata gattccttct tttcgttgaa ataccaccaa aatgactttt ttatgtttga
     7861 aaacagcata gtccatatct ccaattgcag aatagtttgt actgcgcatt gacagcatag
     7921 ttgaaataag aaaaaactcg tttcttactt gaccagcttt taacattggg tttgttcctt
     7981 gttttattat tcctgtcaga gttctcccat attcattaat cagtcctacg tatcttattg
     8041 actttgaaat tagcagaatt ttttgacatt tctttctata cgctatgatc tcatctttcc
     8101 atttttgatc ttgtgttttt atcacaaaat agattggtaa tcagagttaa taaagttgga
     8161 cttatttttt cttggttttt tctaaatttt gttttagtgt tgcgttttct ttttttagct
     8221 gttcgttttc tgattctaat gcattcattg aattcatgga gtgcattgga tgacctggtc
     8281 caaactgact tgatgacggc aataaatcaa aaagatttga tgcgtattgt tgaaatgcag
     8341 tatgaaaacc agaaagtctt gcaagcattg atgcattatc acgaattatt ttctctttga
     8401 gcatacctgt ttttgatatt cctgatggat gatttgacat ctgtggtaat ctcggaaatg
     8461 ccaacggtga attcatcatg gttatgtcta tcccatacac ttcaagtatt ttttttgggt
     8521 cgtgatacca catatctgat tgtttatgac actcatattt catctttcct tgcatgaatt
     8581 acgcgttaca tatgtcatcg tgtcaatcat gactctattt ttatcaaatc cagttgatcg
     8641 ctgatcctta tagaaggcac tgatcttttc caataaaatg ggaaaaacaa tatcaaagca
     8701 ttgtatgaaa tgtaaaaaaa gtattgtttc aacagatttg tataagatag taatgtatat
     8761 tgtcggtgaa aaatttaccg atcaccatta tgaacacata gaatgtcctg acagacttac
     8821 aatctaacca atatatgcaa agacacgata gacctttccg gtttctctgt cacgatagtc
     8881 ccaatggcct tcattgcctt ttacttcatt gaaatcttgt tttaatgatg gatgtagttt
     8941 ttcataaaca tcttcaccga ctagaatctg atttggtttt gcaagactgg tgattttagc
     9001 tgcaatactc attccagggc ccaaaatatc aacgtgtgac ttgatttcat ctgcaccata
     9061 tctgactatg gtgttttctc cgaaatccat cccaatcttt accttaagat cagggtaatc
     9121 atactgatta agaattggat tgattcctcg ttcgattact gatatcatcg actttccaca
     9181 attgactgca ttgtctgcag ctactagtgg attgtctact gccacaaaat atccaataac
     9241 tgcatcgcct acatatttca aaacatgtcc ttcatgatgc tttataacaa atcccatctc
     9301 ttgtgaaaat gaactgatta ttattgccaa cttttctgct ggcaattcaa gagttaattg
     9361 agtagatcct accaaatcga cataaagaac aatcattttc actcttgagg aaacgtgttg
     9421 gcgcaaaaac ttgtccgaat cgtctcgaga gtaatcatat tcataacctt tctttaaaga
     9481 actccagacg cgtttttgga cttctttgat cagggtttcc gtatctacga tctcagaacg
     9541 gttgcctccc atcatcatat ctacaatatc tttggttttc attggttttg gaggctcttg
     9601 tggtgtttct tcgctcataa tttatctcat accatctatt ctagtggaaa atttactcca
     9661 catattggac attgtgcacg ctcacccttg tatttgaggt caaaatattg aacaatctca
     9721 acatcacaat tccagcaaat tatcttttta tatgaagatt ttggcactat ttttcttact
     9781 acgcgtctga tatttaaagc acatatcaga tttatgtgac tcttaaaaaa agaaacatgt
     9841 gcaaatccca tcaaaatctc aagatgagtt tattaaaaaa atgactgaag aaagtgaaaa
     9901 ggtaagatca gcattcttag aaaaaattac agaacacaca aagaatgtac caattaaagt
     9961 catgcttgga gatagtacaa tagttgatca gactacgttt gatccagcac tagtacggca
    10021 ctactatcaa gaaatattaa aaaaaatatc agattggaaa actcaaggga tatctgttgt
    10081 aaatgataag gatctaagac gaatttttct caaattcgag atacaagaag gaaactatct
    10141 tctttcttgt catatgtcat tacaatatca tgcgttgcta tattacaaac caaatcatcg
    10201 agttattgaa atacaaaagg aattatctga aatagttgat aaaaccaaaa acttggaaac
    10261 tcagattgct gatcaaggaa acaagataat ccaagaaaaa ttaaaagaaa tcggacaaga
    10321 aaacgctgat caacaaaaga tttttgaaat tttctttcaa aatgataatt tggcaaatac
    10381 acttgttgat aagatatatt caagcgctga tgaaaacttt gaacaacttt caaaaagaaa
    10441 atccgatcta tttaaagaac ttgataatat gttgatggaa atatatcata catcaacgac
    10501 tctgattgat gaaatgagaa tgatatctgc tgaagaggga tgtttatgtc attttgatct
    10561 agaacatttg aaaaaagacg ggaaggaggg cacatttgat
//
LOCUS       DATIDL010000111         2767 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrosopumilaceae archaeon isolate SMAG_U16342
            k141_2364271, whole genome shotgun sequence.
ACCESSION   DATIDL010000111 DATIDL010000000
VERSION     DATIDL010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935836
            Sequence Read Archive: SRR25158326
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosopumilaceae archaeon (soil metagenome)
  ORGANISM  Nitrosopumilaceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrosopumilales;
            Nitrosopumilaceae.
REFERENCE   1  (bases 1 to 2767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/04/2024 05:09:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,520
            CDSs (total)                 :: 1,474
            Genes (coding)               :: 1,474
            CDSs (with protein)          :: 1,474
            Genes (RNA)                  :: 46
            rRNAs                        :: 1 (5S)
            complete rRNAs               :: 1 (5S)
            tRNAs                        :: 43
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2767
                     /organism="Nitrosopumilaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2364271"
                     /isolate="SMAG_U16342"
                     /isolation_source="soil"
                     /db_xref="taxon:2202732"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(59..700)
                     /locus_tag="VLB45_04995"
     CDS             complement(59..700)
                     /locus_tag="VLB45_04995"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HSD05093.1"
                     /translation="MDVSEYWRNRGPRYFEELQNQPKRLRSRLKDQEDHLVRLLQPFR
                     FNSILEIGCGSGRYTKILYNLYEPKVYTAIDISKEQIENAKKYVFNDKINFQCAQIQN
                     LSLEEKFDLVFASEVLMHIDSKDIDHVIKKLISFSTNKIISIDWFNEKNIGKKLGGYC
                     YMHDYKTLFKKNGAKIVKIHMLPLSLSWKLLSVYTQLRGRDSVETQAIFEVDV"
     gene            843..1619
                     /locus_tag="VLB45_05000"
     CDS             843..1619
                     /locus_tag="VLB45_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose dehydrogenase"
                     /protein_id="HSD05094.1"
                     /translation="MRKDLVAGLGEIGAPMLKLISKKFTAVGYDINPKLMNKKQFDKH
                     TDLDTCFLHICIPFTKKFIPNVLSLYRKFKPEGIVIHSTISPGTTHLIQSKLPAPIIY
                     SATRGVHKRMLYDLKRYTKFYAIEPSAPRANWASSEYSKLMKKCGVKTKKMSNPLTLE
                     LAKIVCDTSYYGWLINYAQLSNMIAIKNKVNYDEMWSFADEIHKYLGNRPKMFPGFIG
                     GHCVIPNLDLINDETLDLIKEINLDYAKILKKRQARGKKY"
     gene            1616..2677
                     /gene="mtnA"
                     /locus_tag="VLB45_05005"
     CDS             1616..2677
                     /gene="mtnA"
                     /locus_tag="VLB45_05005"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HSD05095.1"
                     /translation="MKQQTKIYSSLRTVEWKNNTVVMIDQTKLPNKLVFVKYTDYKDV
                     ANAIKTLVVRGAPAIGVSGAFGLALAALQSKAKTREKLLDDLEKARKILFETRPTAVN
                     LAWGLDKIMKVAKKGNDITTIKENIIKTAKKMADEDIQTNMRMGKHGATLFESNDTIM
                     THCNAGALATVGYGTALGVIRATKESGKKIKVIATETRPVMQGSRLTAFELKHDGIDV
                     SLIPDTAVGYAMAKGLVNKVIVGADRILRTGHVYNKIGTYQVAMMAKQHKIPFYVAAP
                     LSTFDLKSNPKDVIIEQRKASEVTGVAGKKTAPDDIAVINPAFDMTPPELITGIITEA
                     GVAKTPFESSIKKLFKSKL"
BASE COUNT         1002 a          461 c          464 g          840 t
ORIGIN      
        1 catcttattc cctctccgtt ttatactgac taatacagat ttagtttttg ataacgaatt
       61 aaacatcaac ttcaaaaata gcttgtgtct ccacactatc cctgcctctt aactgtgtat
      121 aaacactcaa caatttccat gagagtgaga gaggtagcat gtgtattttt acaatctttg
      181 caccattttt tttgaaaagt gtcttataat catgcatata acaatatcca cctaattttt
      241 taccaatgtt cttttcattg aaccagtcaa ttgaaattat tttattggta gaaaatgaaa
      301 tcaatttctt aattacatga tctatgtctt tagaatctat atgcatcaaa acttcactcg
      361 caaagaccag atcaaatttt tcttctaaac ttagattttg aatttgtgca cattgaaaat
      421 taattttgtc attaaatacg tatttttttg cattttctat ttgttcttta gatatgtcta
      481 tagctgtgta tacttttggt tcataaagat tgtatagaat ttttgtatat ctgccagaac
      541 cgcatccaat ttccaatatg ctgttgaatc taaaaggttg caataatctt acaagatgat
      601 cttcttggtc ttttaatcta gatcgtaaac gtttcggttg attttgtaat tcttcaaaat
      661 atcttggtcc acgattcctc caatattcag atacatccaa aactaatcta gtatgaacta
      721 tgactttagt tataaaaatc gttgtctttg catagccaca tactcaatca ttgttaaaca
      781 cattagtgaa gaatgtgatt tttggtattc ttaataaaag gcaaacaaat tctgcatgtg
      841 tattgagaaa agatctagtt gcagggttgg gtgaaattgg agctcctatg ctaaaattaa
      901 tctctaagaa atttacagca gtaggttacg atatcaatcc aaaattaatg aataaaaaac
      961 aatttgacaa acatactgac cttgacactt gttttttaca tatctgtata ccttttacaa
     1021 aaaaattcat tcctaatgta ttatcacttt atcgcaaatt caaaccagaa ggaatagtca
     1081 ttcatagtac gataagtcct ggtactactc atcttataca atctaaactt ccagcaccca
     1141 ttatttacag tgcaacaaga ggagtacaca aaagaatgct ttatgatcta aaacgatata
     1201 caaaatttta tgcaatagaa ccaagtgcac ctagagcaaa ttgggcatct tcagaatatt
     1261 ctaaattgat gaaaaaatgc ggagtcaaga caaagaaaat gtcaaaccca cttacattag
     1321 agcttgcaaa aatagtgtgc gatacttcct attatggctg gctgataaac tatgctcagc
     1381 ttagtaatat gattgccata aaaaacaaag taaactatga tgaaatgtgg tcttttgcag
     1441 atgaaattca caaatattta ggaaataggc caaagatgtt tccaggattt attggaggac
     1501 attgtgtaat tcccaatctt gatttgataa atgatgagac acttgatctt ataaaagaaa
     1561 ttaatttaga ttacgcaaag attctaaaaa aacgtcaagc ccgtggtaag aaatattgaa
     1621 acaacaaaca aaaatttatt ctagtcttag aacggtagaa tggaaaaata atacagtggt
     1681 aatgatagat caaaccaaac tgccaaacaa acttgttttt gtaaaatata ctgattacaa
     1741 ggatgtagca aacgcaatca aaactcttgt ggtgagaggt gctccagcta ttggcgtatc
     1801 cggtgctttt ggcctggctt tagcagcatt acaaagcaaa gcaaaaacta gagaaaaact
     1861 attagatgac ttggaaaaag caagaaaaat tctctttgag acaagaccaa cggcagtaaa
     1921 tcttgcttgg ggtcttgata aaataatgaa ggttgcaaaa aaaggtaacg acattactac
     1981 aattaaagaa aatatcatta aaacagccaa aaaaatggca gatgaagata ttcaaactaa
     2041 tatgaggatg ggaaaacacg gagcaacatt gtttgaaagt aatgatacta taatgactca
     2101 ttgtaatgca ggagcattgg ctactgtagg ttatggaaca gcattaggtg ttattcgtgc
     2161 aactaaagaa agcggaaaaa agatcaaagt tatagctact gaaacaagac cagtaatgca
     2221 aggctctaga cttactgcgt ttgaattaaa acacgatggt attgatgtta gtctgattcc
     2281 agacactgca gtaggatatg ctatggcaaa gggattggta aacaaagtaa tagtaggagc
     2341 agacagaatt ctgcgtacgg gtcatgtcta caacaagata ggaacatatc aagttgccat
     2401 gatggcaaaa cagcacaaaa tcccatttta tgtagctgcc ccactttcta cctttgatct
     2461 aaaaagtaac cctaaagacg tcataataga acaacgcaag gcaagcgaag ttacaggagt
     2521 cgcaggcaaa aaaaccgcac cagatgatat tgctgtgata aaccctgcct ttgacatgac
     2581 tcctccagaa ctcattactg gtattataac agaagcggga gtagcgaaaa caccatttga
     2641 aagttcaatt aaaaagttgt tcaaatcaaa gctgtaaggt atttatttca tacgtgattg
     2701 aaccaatttt atgggtacag tgacatctca acaaattcta gattctctaa gacaatgcat
     2761 ggatcct
//
LOCUS       DATIDL010000112         3252 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrosopumilaceae archaeon isolate SMAG_U16342
            k141_2972301, whole genome shotgun sequence.
ACCESSION   DATIDL010000112 DATIDL010000000
VERSION     DATIDL010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935836
            Sequence Read Archive: SRR25158326
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosopumilaceae archaeon (soil metagenome)
  ORGANISM  Nitrosopumilaceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrosopumilales;
            Nitrosopumilaceae.
REFERENCE   1  (bases 1 to 3252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/04/2024 05:09:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,520
            CDSs (total)                 :: 1,474
            Genes (coding)               :: 1,474
            CDSs (with protein)          :: 1,474
            Genes (RNA)                  :: 46
            rRNAs                        :: 1 (5S)
            complete rRNAs               :: 1 (5S)
            tRNAs                        :: 43
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3252
                     /organism="Nitrosopumilaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2972301"
                     /isolate="SMAG_U16342"
                     /isolation_source="soil"
                     /db_xref="taxon:2202732"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            357..773
                     /locus_tag="VLB45_05010"
     CDS             357..773
                     /locus_tag="VLB45_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007550286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HSD05096.1"
                     /translation="MADAKIAESKLHDITHFGIRAALGVIFIVHGLGKFEPGFAGFLT
                     NINIPIEMQTLLALAELVPGILLLIGVLTRISASVLSVVMLGAIFHVKHAANLTGQGG
                     FEIDLILLASCLATIVAGPGRVSISHVAKRIPRFLQ"
     gene            complement(815..946)
                     /locus_tag="VLB45_05015"
     CDS             complement(815..946)
                     /locus_tag="VLB45_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05097.1"
                     /translation="MKARFNTSCVACGEQIKTGKEIAKDQTGRWVHKHCAPEIIDLP"
     gene            1101..1229
                     /locus_tag="VLB45_05020"
     CDS             1101..1229
                     /locus_tag="VLB45_05020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05098.1"
                     /translation="MQQYQFIECQACGSPSARVEEEPKYHGLRGRCPDCGGDWPES"
     gene            1272..2072
                     /locus_tag="VLB45_05025"
     CDS             1272..2072
                     /locus_tag="VLB45_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HSD05099.1"
                     /translation="MENLYENAANDFLELASEQRLNILFNLLSNRSKISNMAKEVGAT
                     VQEVHRNFERLADAGLISKDKDGYYGLTTYGKTICTQVPDLVFVSKNRKYFETHDFGD
                     IPMKFIQRIGALAAGQHIKGFVKVMEQWKTIYKNADEYIFEILSEVPLDLIEPIISRI
                     KHGIKFNYIFSESTIIPKGRKQLLEKLGFKKLLEQGLVERKMQKNVNVVVVLNEKEAC
                     VMFPTIDGDADIGEMFYSRDHLFHEWCLDYFRYCWYGSDIFQESKLKE"
     gene            complement(2074..>3252)
                     /locus_tag="VLB45_05030"
     CDS             complement(2074..>3252)
                     /locus_tag="VLB45_05030"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HSD05100.1"
                     /translation="ALANSFNLMSKSLRKSTILLSKTEQQYKKLYEGSPTLLRTIDLN
                     GIVIDCNESYANQLGYTKDELIGRSIFETTAEKSIEQMHESLEIWKNTGHVENREIWL
                     KKKNDMSFPALLSATNLYDENGKLIGSNTAIRDISDIYYARKKLEENEIQIREQYEQL
                     KNVNKIKEEFLAMITHELKTPLVPIKGYIDMLLSEQLGSFNETQKQKLEIIRSSTNSL
                     LNIISDLLDAQKIELGQLRLSKNKQDVTELINATITKLKLDLEHGGIDITTELESVSC
                     NCDKVRIEEVLGNLITNAIDFCPKTNGKVQIKSHLEDPKHVKITVKDNGIGIQKDKLG
                     KIFVKFYQIDASTTREHGGTGLGLSVCKGIIESHGGKIWAESEGRGKGTTIHILLPLE
                     "
BASE COUNT          978 a          579 c          589 g         1106 t
ORIGIN      
        1 aatcaaagtt atactggcct taggaattct cagcagagga ggttttttct aaattggctg
       61 aggaactgtg tagaaagtgc ggagaagcgt tacaagaatt ttctttttgt gtgcgatgca
      121 agcaattgat acaacaaata tgtagcgtat gtggaagaaa aactgaagag cggtttcatc
      181 aatactgttc ataccagcta caggtgctca aactgttccc aataccgcct gaccatggcc
      241 atatgtataa actaaaaatg gatccaacgg ttcattttat aaaataattt tagatcctgt
      301 tcttgtaaac taattatgta gacaaggttt ttaagtttag aaaatttttt atcatcatgg
      361 ctgatgcaaa aatagctgaa agcaagttac atgacattac ccactttggc atcagagctg
      421 ctttgggtgt gatctttatt gtacatggtt tgggaaaatt tgagccgggt tttgcaggat
      481 ttctaacaaa tatcaatatc ccaatagaaa tgcaaactct tcttgctctt gcagaactgg
      541 tccctggaat tctactacta ataggagtct tgaccagaat ttcagcgtct gttttgtcag
      601 tggtcatgct tggtgctata tttcatgtaa aacacgctgc aaaccttaca ggtcagggag
      661 gatttgaaat tgatctcatt cttcttgctt catgtttggc cacaatagtc gcaggaccag
      721 gcagggtttc aatatctcac gttgcaaaaa gaataccacg attcttacaa taggtatttt
      781 tttaaaattt agcctggtac aataagaaac tattctatgg cagatcaatt atttctggag
      841 cacaatgctt gtgaacccat cttcctgttt ggtccttcgc aatttcctta cctgttttta
      901 tctgctcgcc acacgctacg catgacgtgt taaatcttgc tttcatgact ttttgttgct
      961 aatatgtcat aaaagtactg gcattgaaag ggtacaaacc aaaggtcagt tatttggtgc
     1021 tttttggata agctaggagt attattcttc agctagtttg ttgaatcctg gtaatataca
     1081 aaattgtacc attgcttgtc atgcaacaat atcaattcat agaatgccaa gcatgtggtt
     1141 cacccagtgc tcgtgtagaa gaagaaccaa aataccatgg actaagagga cgctgtcctg
     1201 actgtggtgg cgactggcca gaatcataaa ctctacgtaa agtttagatt cgtattttga
     1261 ttagctcatg cgtggaaaat ctttatgaaa acgctgcaaa tgattttcta gagcttgcta
     1321 gtgaacagag actaaacatt ttgtttaacc ttctttcaaa cagatcaaaa atttctaaca
     1381 tggcaaaaga agtgggagcc actgttcaag aagtacatag gaactttgaa cgtcttgcag
     1441 atgctggact gataagcaaa gacaaggatg ggtactatgg acttactacg tatggaaaaa
     1501 ccatatgcac ccaagtgccg gatctagtct ttgtatctaa aaacagaaaa tatttcgaaa
     1561 cacatgactt tggagatatt ccgatgaaat tcatacaaag gattggggca ttagcagcag
     1621 gtcagcacat caaaggattt gtcaaagtta tggaacagtg gaaaacaatt tacaaaaatg
     1681 ctgacgaata tatctttgaa atactgtctg aggtaccatt agatcttata gaaccaataa
     1741 tttcaagaat aaaacatggt ataaaattta actatatttt ttctgaatct actataatac
     1801 ctaaaggaag aaaacaactc cttgaaaagc tcggttttaa aaaactcctt gaacaaggtc
     1861 ttgttgagag aaaaatgcaa aaaaatgtca atgttgtagt cgtcctaaac gaaaaagaag
     1921 catgtgttat gtttccaaca attgatggtg atgccgatat cggggaaatg ttttacagca
     1981 gagatcatct ttttcacgag tggtgtcttg attatttcag atactgttgg tatggttctg
     2041 acatattcca agaaagtaag ctaaaagaat agtctattcc aaaggcaaca aaatatggat
     2101 tgttgtacct ttaccccttc cttctgattc tgcccagatc ttaccaccat ggctttcaat
     2161 tatgcccttg caaactgata gaccaagacc tgttcctcca tgttctcttg tagtagaggc
     2221 gtctatttga tagaacttga caaaaatctt gccaagcttg tctttttgta tcccaatgcc
     2281 gttgtctttt acagtaattt ttacatgttt aggatcttct agatgtgatt ttatctgaac
     2341 tttaccattt gtctttggac aaaaatctat ggcatttgta atcagatttc ctaagacttc
     2401 ttctattcta actttatcgc aattacaaga aacgctttca agctcagtgg taatgtctat
     2461 tccaccatgt tctaaatcca gctttagctt tgtgattgtt gcatttatca actctgtaac
     2521 gtcttgtttg ttttttgaca gtcttaactg accaagttcg attttttggg catctaacaa
     2581 atctgatatt atatttaaaa gtgaatttgt actggatctg atgatctcaa gtttctgttt
     2641 ttgagtctca ttaaaagaac ctagctgctc tgacagcaac atgtcgatgt atccttttat
     2701 tggaacaagc ggagttttta gctcatgcgt tatcatggcc aaaaattcct cttttatctt
     2761 attgacattt tttagttgtt catattgctc tcgtatttgt atctcgtttt cttccaattt
     2821 ttttcttgca tagtaaatgt ctgatatatc tctgattgca gtattacttc caatcaattt
     2881 tccattctca tcatataagt tggtagcact aagtaatgct ggaaaagaca tgtcattttt
     2941 tttcttcaac cagatctctc tattttctac atgacctgtg tttttccaaa tttctaatga
     3001 ttcgtgcatt tgctcaatac ttttttctgc agttgtctca aatattgatc ttccaataag
     3061 ttcatctttt gtatacccta attgatttgc ataagattca ttacaatcaa taacaattcc
     3121 gttaagatct attgtacgaa gaagagttgg tgaaccttcg tacaactttt tgtattgctg
     3181 ttctgtcttt gaaaggagaa tagtagattt tctaagtgat tttgacatca aattaaacga
     3241 attggcaaga gc
//
LOCUS       DATIDL010000113         4874 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrosopumilaceae archaeon isolate SMAG_U16342
            k141_1559200, whole genome shotgun sequence.
ACCESSION   DATIDL010000113 DATIDL010000000
VERSION     DATIDL010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935836
            Sequence Read Archive: SRR25158326
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosopumilaceae archaeon (soil metagenome)
  ORGANISM  Nitrosopumilaceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrosopumilales;
            Nitrosopumilaceae.
REFERENCE   1  (bases 1 to 4874)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4874)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/04/2024 05:09:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,520
            CDSs (total)                 :: 1,474
            Genes (coding)               :: 1,474
            CDSs (with protein)          :: 1,474
            Genes (RNA)                  :: 46
            rRNAs                        :: 1 (5S)
            complete rRNAs               :: 1 (5S)
            tRNAs                        :: 43
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4874
                     /organism="Nitrosopumilaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1559200"
                     /isolate="SMAG_U16342"
                     /isolation_source="soil"
                     /db_xref="taxon:2202732"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..375
                     /locus_tag="VLB45_05035"
     CDS             <1..375
                     /locus_tag="VLB45_05035"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007550970.1"
                     /note="catalyzes the formation of
                     5,10-methenyltetrahydrofolate from
                     5,10-methylenetetrahydrofolate and subsequent formation of
                     10-formyltetrahydrofolate from
                     5,10-methenyltetrahydrofolate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylene-tetrahydrofolate
                     dehydrogenase/5,10-methylene-tetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HSD05101.1"
                     /translation="RSNLVGKPLYHLLLEKNATVTTCHSKTKNLKEHCQRADIIITAI
                     GDRSKFVLTADMIKEGAIVIDVGISRQNDKVVGDTDYTEIIKKAAYATPVPGGVGPMT
                     IAMLLKNTVTAASISKGFGSRG"
     gene            386..922
                     /locus_tag="VLB45_05040"
     CDS             386..922
                     /locus_tag="VLB45_05040"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010189788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HSD05102.1"
                     /translation="MRKQLLDSRDALSQDFIDIISRQIQDNLRKVDAYRDAQSIGAYY
                     SIGSEVKTQSILQDILKSQKELSLPKVVKKDLVFKKITSLSDLEQGNFSVMEPKENCP
                     IMKKIDAIIIPAIALTKDGYRLGYGFGYYDRYLSSKKMKKIALCYSKQIIKSFPSSEH
                     DVKMDYIVTEDEIIVSER"
     gene            complement(911..2170)
                     /gene="purD"
                     /locus_tag="VLB45_05045"
     CDS             complement(911..2170)
                     /gene="purD"
                     /locus_tag="VLB45_05045"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016939284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HSD05103.1"
                     /translation="MTDILVVGSGGREHALGWKLSQSNKVGKVYHAPGNGGTYNNISI
                     QVEEIDKLAKFASEKNCLTVVGPEVPLSHGIVDEFTRQNLRIFGPTKNAAQLESSKVW
                     AKNFMRRNQILTARFEIFDSAKEAREYAKSVDFPLVVKADGLAAGKGVIVCNDSDDAV
                     SAIDKMLVEKSFGSAGSKIILEERIDGVEASYIALSDGYTAIPMATSQDHKRIYDDDK
                     GPNTGGMGAYSPTPVIDDSLIKEIQKNVIEKTINSMKSESILFKGFLYAGVMIKDGKP
                     YVLEFNARMGDPECQPILMRMDSDLYDYINASIDGTLSRLPPISWKKQSAVCVVLASK
                     GYPDSYLKNEEISGLDLEDKNSFVFHAGTKLENNKVLTNGGRVLGVTALGDTLQSAIS
                     NAYSRAEKISWKSKYCRADIGKKGLSF"
     gene            2252..2551
                     /locus_tag="VLB45_05050"
     CDS             2252..2551
                     /locus_tag="VLB45_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05104.1"
                     /translation="MSQIDQQKSVYLFVHGRGDLEPSAINALISNGFSKDKIISAKPD
                     KVGNVGDYMAMLWMPPNPDHIKIQKITNVEKVSPEGMIGLWKGVSKEDLFQIKLK"
     gene            complement(2554..2850)
                     /locus_tag="VLB45_05055"
     CDS             complement(2554..2850)
                     /locus_tag="VLB45_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007401905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05105.1"
                     /translation="MKNSQLDAVVSGKGVKLHRFEPSQRQIWTVVGKENEHWLDPDLR
                     FCSCEDYFFNTLNGGSECYHLKSVNIAKEQNSVEIITFSDDEFANFISALISDL"
     gene            2886..3629
                     /locus_tag="VLB45_05060"
     CDS             2886..3629
                     /locus_tag="VLB45_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HSD05106.1"
                     /translation="MVQTRIVQSHAALILEEEKKHLVVSDLHIGFESTFAVNDIYIEP
                     AIVVEETSNEIISIAESEKVDSLILLGDIKSGIDSISKIEWEAVPRFFEIAKKISTIL
                     IPGNHDGNIQYLLPDSVKVASSSGIVLGDTLLTHGHTLPSENLSHINKIIMGHVHPVF
                     FQEGSVLDGQRVWVSLKVEKNRLFPASKGELEILIMPTFNKYFYANHKKYKKSISPIL
                     EHIKDFQSAKIVTLDGVIIGNESMLENVI"
     gene            complement(3626..>4874)
                     /locus_tag="VLB45_05065"
     CDS             complement(3626..>4874)
                     /locus_tag="VLB45_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007550901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HSD05107.1"
                     /translation="DRAENRSKFSQVLDKLHIMQPQWQAFSSLPDAKKFANKVGYPVL
                     VRPSYVLSGAAMKVVWSQEQLKKYLSEATNVSPDYPVVITKFMLNSLEVEVDGVCDGK
                     NVIIGAIVEHIESAGVHSGDSMMCIPPWRLSNKIIETITDYTTKIATAFSIKGPFNLQ
                     FLVNSDQVYVIELNIRASRSMPFVSKFVKMNLITLAAKAVLGKSLPKIPKDRWKKIHS
                     YGIKVPQFSFMQLEGADIILGVEMQSTGEAACFGDSFHDALSKGLISVGYSLPKEGTA
                     LITVGGSENKEKLLQTVLLLKSLGYKILATEHTAEFLSDKRIGNVDVVYKISEPQRKP
                     NIADLLYERKINFIVNVPSTATLEKYVGMLYDEYQIRRKAVELGIPVLTTTELADSFV
                     KTLEWLGSNDPTKKPLDPYDSFN"
BASE COUNT         1558 a          934 c          861 g         1521 t
ORIGIN      
        1 cgaagcaatc tagttggaaa gccgttatac catttacttc ttgaaaaaaa tgcaactgtc
       61 actacatgcc attcaaagac aaaaaacctc aaggaacact gccaaagagc agacattatc
      121 attaccgcga taggagacag atccaagttt gttcttactg cagatatgat aaaagaaggc
      181 gcgatagtaa ttgatgttgg cataagcaga caaaatgaca aagttgttgg ggatacagat
      241 tatactgaaa ttatcaaaaa agctgcatat gccacaccag ttccaggagg tgtaggacca
      301 atgacaattg caatgctttt aaaaaatact gttaccgcag cttcaatcag taaaggattt
      361 ggttcaagag gttgaaaaat ccaggttgcg aaagcaacta ctggattcac gtgatgcatt
      421 atctcaagac tttatagaca tcataagcag acaaatccaa gacaatctaa gaaaggtgga
      481 tgcttacagg gatgcacaga gtattggcgc atattactcc ataggaagtg aagtaaaaac
      541 gcaaagtatc ttacaagaca tactaaagtc acaaaaagaa ctttcactac caaaagtagt
      601 aaaaaaagat ctggtattca agaagattac tagtctttca gatcttgaac agggaaattt
      661 ctcagtcatg gagccaaaag aaaattgccc aatcatgaaa aaaattgatg caataataat
      721 tccagcgata gcacttacaa aagatggata caggttagga tatggttttg gttattatga
      781 cagatacctt tctagtaaga aaatgaaaaa aattgcattg tgttattcaa agcaaatcat
      841 aaaatcattt ccatcctcag aacatgatgt caaaatggat tacatcgtca cagaagacga
      901 gatcatagtt tcagaaagat agtccttttt tccctatatc agctcgacaa tacttgcttt
      961 tccaagagat tttctcagct cttgaatacg cattagatat tgcagattgt agcgtgtctc
     1021 ctagtgcagt aactccaagt acacgcccgc catttgtcaa tactttgtta ttttcaagtt
     1081 ttgttcctgc atgaaataca aatgaatttt tatcttctaa atcaagacct gatatttcct
     1141 catttttcag atacgagtca ggataacctt ttgatgccaa tacaacgcat actgcacttt
     1201 gtttcttcca tgagataggt ggaagtctgg ataatgttcc atctatgctt gcgttgatat
     1261 agtcgtataa atctgaatcc atacgcatca gtattggttg acattcagga tcacccattc
     1321 tcgcattaaa ttctagaacg tatggttttc catcctttat catcactcca gcatacaaaa
     1381 atcccttaaa cagaatgctt tcgcttttca tagaattaat agtcttctct atcacgtttt
     1441 tttgaatttc tttgattaga gaatcatcaa ttacaggagt cggagaatat gcccccatac
     1501 ctccagtgtt tggccctttg tcatcatcgt aaattctttt atgatcttgg cttgtagcca
     1561 ttgggatagc tgtgtatcca tcagacaacg caatgtatga tgcctcgact ccatcaattc
     1621 tttcttccaa tatgatttta gagcctgcac tgccaaagct tttttcgaca agcatcttat
     1681 ctattgcaga tactgcatca tcagaatcat tacagactat gacacctttt cctgctgcaa
     1741 gaccatctgc ttttactaca agtggaaaat ctacggattt ggcgtattct cttgcctctt
     1801 ttgcactatc aaaaatctca aaccgtgctg tgagaatttg gtttcttctc ataaagtttt
     1861 ttgcccaaac tttacttgat tctagttgtg ccgcattttt tgttggtcca aatattctca
     1921 aattttgcct tgtaaactcg tcaactatac catgtgaaag aggcacttca ggaccaacta
     1981 cagtaagaca atttttttcg gaagcaaact ttgcaagctt atcaatttct tcaacctgaa
     2041 ttgaaatatt gttgtatgtt cctccatttc caggtgcatg atacacttta ccaactttat
     2101 tgctttgaga aagtttccaa ccaagagcat gttctctccc gccagagccc acaactagaa
     2161 tgtctgtcaa caaattagct ttctttatct tgtatataat ccaagattgg ttttagaaat
     2221 attgattaat actagtctct acagctgtca aatgagccaa atagatcaac aaaaatcagt
     2281 ttaccttttt gtccatggaa gaggagattt agaaccttct gcaatcaatg cgttgatatc
     2341 aaatggtttt tcaaaagaca agattatttc tgcaaaacca gacaaggttg gaaatgtagg
     2401 cgattatatg gcaatgcttt ggatgccacc aaatccagac cacatcaaga ttcagaaaat
     2461 aactaatgtt gagaaagtaa gccctgaggg catgataggt ctatggaagg gtgtctcaaa
     2521 agaagacttg ttccaaataa agctaaaata gactcaaaga tcagaaatta acgctgaaat
     2581 aaaattagca aattcatcat ctgaaaatgt tattatttcc acactgtttt gctctttagc
     2641 aatgttaaca gattttaggt ggtaacattc actgccgcca tttagtgtat taaaaaaata
     2701 atcttcacat gaacaaaacc tcagatcagg atcaagccag tgttcatttt cttttcccac
     2761 aaccgtccag atttgacgct ggcttggttc aaatctatgt agttttacac ctttaccaga
     2821 aactactgca tccaactgtg agtttttcac aaggatttaa ttcaaaattg actcgtatat
     2881 ctttgatggt gcagacaaga attgttcaat cacacgctgc cttgatttta gaagaagaaa
     2941 agaagcatct tgttgtaagt gacttgcaca taggttttga aagcacgttt gcagtaaatg
     3001 acatttacat agaacctgcc atagttgtgg aagaaacaag taatgaaatt atttcaatag
     3061 ctgaatctga aaaggttgat tcgctgatac tcttaggaga cataaaatca ggcatagatt
     3121 caatatcaaa aatagaatgg gaggcagtac ctcgtttttt tgaaattgca aaaaaaatca
     3181 gtacaatttt gattcctggg aatcatgatg gaaatataca gtatctttta ccagacagtg
     3241 tcaaagtcgc tagctcgtca gggattgttt taggagatac cttgttaact catggacaca
     3301 cgttaccgtc tgagaatcta tcacacataa ataagataat catgggtcat gtccatcctg
     3361 tttttttcca agaagggtcg gtattggatg ggcaaagagt ttgggtttct ttaaaggtag
     3421 aaaaaaacag gctctttcca gcatcaaaag gagagttgga aatactgatc atgcctacat
     3481 ttaacaagta tttttacgct aaccacaaaa aatacaaaaa atcaatttca ccaatattag
     3541 aacacataaa agatttccag tctgcaaaaa ttgtaacact agatggagtc ataattggaa
     3601 atgaatccat gcttgaaaat gtgatctagt taaatgagtc gtatgggtca agcggttttt
     3661 ttgtcggatc attacttcct aaccactcta gtgtctttac aaaagaatct gccaattcag
     3721 tcgttgtaag tactggaatg ccaagctcaa ctgctttacg tcgtatttgg tattcatcat
     3781 ataacatgcc aacatatttt tccaaagtag cggtgcttgg aacattgact atgaaattga
     3841 tctttcgttc gtaaagtaaa tcagcaatgt ttggttttct ttgcggctca ctgattttgt
     3901 atacaacatc aacatttcca attcttttat cagacaaaaa ctctgcagtg tgttctgttg
     3961 caagtatctt gtagccaagt gattttaata acagtacagt ctgcaagagt ttttctttgt
     4021 tttcagagcc accaacagtg ataagtgctg tgccttcttt tggcaatgaa tatcctaccg
     4081 agattaatcc ttttgataat gcgtcatgaa agctatctcc aaagcatgct gcctcgccag
     4141 ttgattgcat ctcaacacct aaaatgatgt cagctccctc aagctgcata aatgaaaact
     4201 gtggcacttt gattccgtat gagtggatct ttttccacct gtcctttggt atttttggaa
     4261 gcgatttgcc aaggactgct tttgctgcaa gagttatgag attcattttt acaaatttgg
     4321 aaacaaaagg catcgaacga gatgcacgaa tgtttaattc tatcacatag acctgatcag
     4381 aattgaccaa gaattgtaag ttaaatggtc cttttattga aaacgctgtg gcaatttttg
     4441 tcgtatagtc tgttatggtt tcaatgattt tgttactcaa cctccaagga ggaatacaca
     4501 tcatcgaatc accagaatgc accccagcac tctcaatatg ctcaactata gccccaatga
     4561 ttacattttt tccgtcacag acaccgtcaa cttctacttc aagggagttt agcataaact
     4621 ttgtaatcac tacaggataa tctggtgaaa cattagtggc ctcacttagg tattttttta
     4681 attgttcttg agaccagaca actttcattg cagccccact taggacgtat gatggcctga
     4741 ccaaaacagg ataaccgact ttatttgcaa attttttggc atcaggtaag cttgaaaatg
     4801 cttgccattg aggttgcatt atgtgtaatt tgtctaaaac ttggctaaac ttggaacgat
     4861 tttctgccct gtca
//
LOCUS       DATIDL010000114         3138 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrosopumilaceae archaeon isolate SMAG_U16342
            k141_550821, whole genome shotgun sequence.
ACCESSION   DATIDL010000114 DATIDL010000000
VERSION     DATIDL010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935836
            Sequence Read Archive: SRR25158326
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosopumilaceae archaeon (soil metagenome)
  ORGANISM  Nitrosopumilaceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrosopumilales;
            Nitrosopumilaceae.
REFERENCE   1  (bases 1 to 3138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/04/2024 05:09:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,520
            CDSs (total)                 :: 1,474
            Genes (coding)               :: 1,474
            CDSs (with protein)          :: 1,474
            Genes (RNA)                  :: 46
            rRNAs                        :: 1 (5S)
            complete rRNAs               :: 1 (5S)
            tRNAs                        :: 43
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3138
                     /organism="Nitrosopumilaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_550821"
                     /isolate="SMAG_U16342"
                     /isolation_source="soil"
                     /db_xref="taxon:2202732"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1037)
                     /gene="cysS"
                     /locus_tag="VLB45_05070"
     CDS             complement(<1..1037)
                     /gene="cysS"
                     /locus_tag="VLB45_05070"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HSD05108.1"
                     /translation="MKIFDTLTEKERELETAGNVRIYLCGVTVYDESHIGHARTIIVF
                     DTLRRFLESKGVKVQFVQNFTDVDDKIINRAKQENATAFDISTKYITHYFDDFDRLNI
                     KRADNYPKATEHIQDMINLIKDLVAKNYAYVSKNGVYFSVTKFKEYGKLSKKKIDELV
                     SGARIEVDETKNDPLDFALWKFSDEHPKWPSPWGEGRPGWHIECSAMSLKYLGENFEI
                     HGGGRDLIFPHHENEIAQSESHTGKPFAKIWMHVGMMTINGEKMSKSLGNIKSVDHVL
                     KQWGPNIIRLFCLSGHYTKPIDYSEDLLKENITKWRQVENCYYELALSEGTGPIDEAK
                     AIISEVKKEFE"
     gene            complement(1057..1845)
                     /locus_tag="VLB45_05075"
     CDS             complement(1057..1845)
                     /locus_tag="VLB45_05075"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008299286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HSD05109.1"
                     /translation="MNQSLLDELIKKDYSTIQNKIEAFLKDEVSQRQASGAIFGLSGG
                     IDSAVIAGLCARSIKEKSLALLMPDSKITPRKDTEDALNLVDKLGLEYKLIDINPIHY
                     EYSKYIEPNPLALGNLRARIRASILYYYANSKNYLVLGSSDKSEYLIGYFTKFGDGGA
                     DLLPIVSLYKTQIRQFAKFLGLPDTIISKKSSPNLWPDHVAESELGVSYEEIDACLHC
                     IVDKNLSVQDTVSSTGIELSIVDKIHKLYKKSEHKRITPKACQI"
     gene            complement(2123..2425)
                     /locus_tag="VLB45_05080"
     CDS             complement(2123..2425)
                     /locus_tag="VLB45_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HSD05110.1"
                     /translation="MVWKKVAEKNAIPEGKGKEFLVDGKHIAIFNQNGFHGLDSICVH
                     QDGSLAPGKLDGDIVECPLHFWHYNIKTGELQDYIKGVKLQTYKVEAKSDGLYVDV"
     gene            2505..>3138
                     /locus_tag="VLB45_05085"
     CDS             2505..>3138
                     /locus_tag="VLB45_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007550766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HSD05111.1"
                     /translation="MSKIKDPRLADKGKLSYEWAKSHMKILTNTVNRLKKSQPLRGLR
                     LGVCLHITKETSVLIMGAKELGADVIVCAANPLSTQDDIAAFLASNGIHIYAWKGQTS
                     KEYDWCIEKVLSHKPNILTDDGSDCHSKVHGNKKYSSLKVIGGTEETTTGVIRLKALA
                     EQKKLRYPVIAVNDAYTKHMFDNRYGTGQSTMDGYLRAMNLLVASKRVVIA"
BASE COUNT          888 a          630 c          558 g         1062 t
ORIGIN      
        1 ctttcaaatt cttttttaac ctcagaaatt atcgcttttg cctcatcaat tggaccggtt
       61 ccttctgata gtgccaattc atagtaacag ttctcaactt gtcgccattt tgtaatgttc
      121 tctttgagaa ggtcttcaga ataatctatt ggttttgtat agtggccaga caggcaaaac
      181 aacctgatta tgtttggtcc ccattgtttt aggacatgat ctactgactt tatgttgccc
      241 aaagattttg acattttttc accatttatt gtcatcatgc caacatgcat ccagattttt
      301 gcaaaaggct ttccagtatg tgattcagat tgcgcaatct cattttcatg gtgaggaaat
      361 atcaaatctc ttcctccgcc atgaatctca aagttttctc ctaggtattt caggctcatt
      421 gcagagcatt caatgtgcca tccgggtctt ccctctcccc agggacttgg ccacttgggg
      481 tgctcatcag aaaacttcca caacgcaaaa tcaagaggat cgttttttgt ttcatccacc
      541 tcgattcttg cccctgatac aagctcgtca atttttttct ttgatagctt gccgtattcc
      601 ttgaattttg ttactgaaaa atacactcca ttttttgaaa catacgcata gttttttgct
      661 acaagatctt tgatcagatt aatcatgtct tgtatgtgct ctgttgcctt gggatagttg
      721 tctgctctct tgatgtttag tctgtcaaaa tcatcaaaat aatgtgtaat gtattttgta
      781 ctgatatcaa acgctgtggc gttttcttgt tttgcccgat ttattatctt gtcatcaacg
      841 tctgtaaagt tttgaacaaa ctgaaccttt actcccttgg attccaggaa tcttcgtaat
      901 gtatcaaaga cgatgatggt tcttgcgtgc ccgatgtggc tttcatcata taccgtaacc
      961 ccacagaggt aaattcttac atttcctgct gtctcaagtt cacgctcctt ttctgtcaga
     1021 gtatcaaaaa ttttcaattt actttctcac tgttttttat atttgacaag cttttggagt
     1081 tattctcttg tgttcgcttt ttttgtacag tttgtggatt ttatcaacaa ttgaaagctc
     1141 aattcctgta gatgatacag tatcttgtac tgacagattc ttgtctacta tacaatgaag
     1201 acatgcatca atttcttcat agcttactcc aagctcagat tctgcaacat ggtctggcca
     1261 aagatttggg ctgctcttct ttgatattat ggtgtctggc aggccaagaa actttgcaaa
     1321 ttgtctgatc tgagtcttgt atagtgatac gattggaagc aagtctgctc caccgtcgcc
     1381 aaattttgta aaatacccaa tcaagtattc gcttttgtca ctagaaccca acacaagata
     1441 gtttttggag tttgcatagt aatacaaaat acttgcacgt atcctcgctc tgaggtttcc
     1501 aagcgctaga ggatttggtt ctatatactt tgaatattca taatgaattg gattgatgtc
     1561 aattaacttg tattctaatc caagcttgtc tactagattg agtgcatctt cggtatcttt
     1621 tcttggtgtg attttagaat ctggcattaa caacgcaaga gatttttctt taattgatct
     1681 tgcgcacagt cctgcaatta cagctgaatc tatgccacca cttaaaccaa agattgcccc
     1741 agatgcctgt ctttgggaaa cctcgtcttt gagaaatgcc tcaattttat tttgtattgt
     1801 actatagtct ttttttatca attcatcaag tagagactga ttcaacaatt cttcaattcc
     1861 gtctaacaga attaaaccta tcaggcatca aaaaaggcaa aacctgtctc tttttgtttc
     1921 aattatgtga tattcctgag tttatatgtc tatgaaagtc gtactagaca tgtttaaaca
     1981 attaattgca aaattgaaat cttgttcttg tagcaagaaa tcacctgacg acataaacgc
     2041 agtaaaagaa tctgaagaat caaagaacta gaataataga ttctattctc tttttgtttt
     2101 tgtaaaaatt gaatcttttt tatcagacat caacatacag gccgtcactt ttcgcttcga
     2161 ccttgtaggt ttgcagcttg actcctttga tgtagtcttg caactcgcca gtcttgatgt
     2221 tataatgcca aaaatgcagc gggcattcta ctatatcgcc gtctagcttg cctggtgcaa
     2281 gagaaccatc ttggtgcacg catatggaat caaggccatg aaatccattc tggttgaata
     2341 tggcaatgtg tttgccgtca actagaaact ctttgccttt tccttcagga atggcatttt
     2401 tttcagcgac ttttttccat accatgattt tcaaactaat ttctgatcaa tatttagatt
     2461 gtcatttgat agcattttat actcctttaa tttgaaacca tttcatgagt aagataaaag
     2521 atccaagact ggcagacaag ggcaaacttt cttacgagtg ggccaaaagt cacatgaaga
     2581 ttcttacaaa tactgttaat cgtctcaaaa aatcacaacc attacgcgga ttaagattag
     2641 gagtttgctt gcatatcaca aaagaaacct cggttttaat tatgggcgca aaggaacttg
     2701 gagctgacgt catagtgtgt gcagcaaatc ctctttccac acaagacgac attgcagcat
     2761 ttcttgcatc aaatggaatc cacatctatg cgtggaaagg acaaacatca aaggaatatg
     2821 attggtgcat tgaaaaggtg ctcagccata aaccaaatat tttaactgat gatggctctg
     2881 actgtcacag caaagttcac ggtaacaaaa aatattcttc gctcaaagtc ataggcggta
     2941 ctgaagaaac tacaacgggt gttatacgac taaaggctct tgccgagcaa aaaaaactgc
     3001 gatatcctgt aattgcagta aatgacgctt acacaaaaca catgtttgat aacagatacg
     3061 gaacaggaca aagcacaatg gacggatatc ttagggcgat gaacttgctt gtcgctagca
     3121 aacgtgttgt gattgcag
//
LOCUS       DATIDL010000115        14807 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrosopumilaceae archaeon isolate SMAG_U16342
            k141_1759158, whole genome shotgun sequence.
ACCESSION   DATIDL010000115 DATIDL010000000
VERSION     DATIDL010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935836
            Sequence Read Archive: SRR25158326
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosopumilaceae archaeon (soil metagenome)
  ORGANISM  Nitrosopumilaceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrosopumilales;
            Nitrosopumilaceae.
REFERENCE   1  (bases 1 to 14807)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14807)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/04/2024 05:09:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,520
            CDSs (total)                 :: 1,474
            Genes (coding)               :: 1,474
            CDSs (with protein)          :: 1,474
            Genes (RNA)                  :: 46
            rRNAs                        :: 1 (5S)
            complete rRNAs               :: 1 (5S)
            tRNAs                        :: 43
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14807
                     /organism="Nitrosopumilaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1759158"
                     /isolate="SMAG_U16342"
                     /isolation_source="soil"
                     /db_xref="taxon:2202732"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            273..947
                     /locus_tag="VLB45_05090"
     CDS             273..947
                     /locus_tag="VLB45_05090"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007550103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HSD05112.1"
                     /translation="MNLQTLVRGINGIIPGGISVKDFSVVTQIDKDTAEKVLDNLMQN
                     GIGRFENDSIYFEENDKLKTSLLAIRMGAAVDEISELLSWQDFEALAEEILRSDNFDT
                     TRNLTLTKPRMEIDVVGIKSDVAILIDCKHWKRSSQAALEKAVKKQIERTKQYLVKEK
                     IRAAVPAIVTLYQEQIKFIDKVPIIPIFQLGSFCEEFYGNLDEMNKLEHGQEEKDGSP
                     YQKDLR"
     gene            complement(891..2654)
                     /locus_tag="VLB45_05095"
     CDS             complement(891..2654)
                     /locus_tag="VLB45_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2070 family protein"
                     /protein_id="HSD05113.1"
                     /translation="MDIENIFPVMTENAENVSNIHRRYSLTLLTPSSPYLSLFVSIAA
                     ASLLVILAELAYFENYDKFFFHMFLTIGVLLVTQILDSILLHTEKSKARHISGFGSLL
                     WFVTALGGIISVPLFSKPDLLPIYLTEGMFLFASFRIGIFTSVIGAKVKLAWAICLIQ
                     PLAMFLAIIPSQFWIPMLADLQAIGYGVVFLVVGTVWSIVTDRAGGKKIPSTHNVLRA
                     YIAAWTRNDSSEMERLMEERSQPSKVSTSQIKFKTRDNEFHLVLPDVHPGPFHPIGGS
                     NIPYLIYKNLNSSAMVMHGISDHSLNLPSKSQVDAYLASFAKSHVLNQGNMCTKPVTV
                     QVNKTRTVGLLFDKTAVLILSLSPHGMEDVPLYVKNELEQYAKNRGFEKLLIVDSHNA
                     MGEEISQNDADDLFKAAKSNLDTLKIKESYPLETGYAHLENTEIKADDLGPSGIGILC
                     LKVNGEKFFIGWSDSNNMQNGLREEVVSYFTKHGYTLIEMCSSDTHYKTKEARNKHGY
                     FTFGSVSTFENVASWYLDLAKKAEQTLSPASFESLQHETDVKVMGTTQLEDYSKSLDR
                     TMRLTQVFLIGTTVFFFLTMF"
     gene            complement(2624..2788)
                     /locus_tag="VLB45_05100"
     CDS             complement(2624..2788)
                     /locus_tag="VLB45_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007401334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit Sec61beta"
                     /protein_id="HSD05114.1"
                     /translation="MSSKKSAPLPASSAGLLRFFEDETKGYKIRPEIVVAMPSGLIAI
                     SWILKIFFLS"
     gene            complement(2831..3160)
                     /locus_tag="VLB45_05105"
     CDS             complement(2831..3160)
                     /locus_tag="VLB45_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sjogren's syndrome/scleroderma autoantigen 1
                     family protein"
                     /protein_id="HSD05115.1"
                     /translation="MSKDLTKKAVEMLLKGATLSSEPCPYCKGVRVMRDGSALCVNCG
                     REAKEENIPIKADKEKTEINTPLAKCEQKLQILTEELEHEKDHQKQQEILKSINELLG
                     IIEKLKK"
     gene            3239..3547
                     /gene="yciH"
                     /locus_tag="VLB45_05110"
     CDS             3239..3547
                     /gene="yciH"
                     /locus_tag="VLB45_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HSD05116.1"
                     /translation="MAVICNTCGLPNDLCACGELEKDATQIVVRVEERRFKKKGTMIE
                     GINPKMNDLGKVVRELKAKYACGGTVKDGYILLQGDHRDTIKGTLVKLGFPEASIEVH
                     "
     gene            3551..4546
                     /locus_tag="VLB45_05115"
     CDS             3551..4546
                     /locus_tag="VLB45_05115"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010195084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HSD05117.1"
                     /translation="MLQSDKLIKALTTPYSALMSVIFGMMIVSFPIGAYVVFNTDIGR
                     EINFEYPLDGINVFLGGIGFKIPFQFELGDGFIVAWSAYLILFSISICGPNKNFIKSV
                     SDIMSGGWENIKNNYLLSMLTWFSILVVFSIIIDSLQQSVGIPIEPPEFSNGLIQFFQ
                     ITVSPLTEEIGFRMSLIGLPLFAMFARKASFTHFLKSLWHPAKNLEITNYKKAIGLVI
                     IVGIFFGVSHIISGTPWSVGKVAQASFGGVIIGWVYMRYGLAPAILLHWATNYFVFSY
                     VFFVSNINQTSITDGFTNPFLGTVEILLIIAGILAATLLVLDYLRTKKESTVTTV"
     gene            complement(4547..5182)
                     /gene="cofC"
                     /locus_tag="VLB45_05120"
     CDS             complement(4547..5182)
                     /gene="cofC"
                     /locus_tag="VLB45_05120"
                     /EC_number="2.7.7.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007550112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043814 - phospholactate
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate guanylyltransferase"
                     /protein_id="HSD05118.1"
                     /translation="MSKFRTGAIVPVKTFSKAKKRLNLPPEKIEILCRLMLNEVLDTI
                     SHSSVIEKIALVTKDENVFEIGKKFGCVEILDEKELGVNNAVSLADKFFSEEGLDATI
                     VFPQDIPLMQAEDIQTLFNFQLSNPCALIVPSRKFDGTNALFRTPTNLMETHYDEDSY
                     KIHLNTAEKHGASSALVLIRRIMLDVDDLNDLKYVSKYSQSFSKIASDLIQ"
     gene            complement(5175..6095)
                     /gene="cofD"
                     /locus_tag="VLB45_05125"
     CDS             complement(5175..6095)
                     /gene="cofD"
                     /locus_tag="VLB45_05125"
                     /EC_number="2.7.8.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008298975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043743 - LPPG:FO 2-phospho-L-lactate
                     transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase"
                     /protein_id="HSD05119.1"
                     /translation="MITILAGGTGSVKLVRGVSSQTRDISVISNVGDNYWLYGLYVCP
                     DIDTILYGLADLLDIDKGWGIRKDTFGFLRQMEILGEETWFKIGDRDVATHLLRTNML
                     KNGKNLTDITAWMCQKFAIDTKIIPVTDNTVETRIVTNKGELHLQEYWVKYRGQDNVE
                     GIKYLGADKARANPAAVNAIHDSKLVIIAPGNPLTSIGPILSIKGIKKELSKSKKKVV
                     VVSPLMGNKAISGPAAKYMEAAGMEVSAYGLAKMYSEVASHMIIDSSDRLLTRKIQNL
                     DMHVYETKIKMKDKIEEDALASFILKQVHV"
     gene            6232..7293
                     /locus_tag="VLB45_05130"
     CDS             6232..7293
                     /locus_tag="VLB45_05130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05120.1"
                     /translation="MSKSIIFSAGLIGFLLVGTISPIWAAQIDVRLSTHLESAQPTFK
                     FLKTVFIEYPDGGELSRTLLGKDILVTLEADKSSPGVSELINKINSNLKELDSTVFVT
                     DLTMTYRAHLVGRGDQTAIDYKVTLEPTIVNYILRQATESSPALIDAQWRGITITGPI
                     TITDAELGDVEVNTLIGFIQKEVPEAYDIISGTEAETLLNANLIDASGVLKQPLANWH
                     HLFDPSGTISDTAKWGYQGEKVVITSFTMGESSLREGMQKEREHSAEFTTDKKYVVRT
                     VESADSANIQIDGFVTETSIEGAEYFGSSPKVPEGYATTSTGGFPVGVMYGMAGAGAA
                     VAVFVLVWSDRKLKKQNRQ"
     gene            complement(7307..7584)
                     /gene="rnpB"
                     /locus_tag="VLB45_05135"
     ncRNA           complement(7307..7584)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VLB45_05135"
                     /product="RNase P RNA component"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00373"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="archaeal type; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00373"
     gene            complement(7646..10000)
                     /locus_tag="VLB45_05140"
     CDS             complement(7646..10000)
                     /locus_tag="VLB45_05140"
                     /inference="COORDINATES: protein motif:HMM:NF012544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HSD05121.1"
                     /translation="MSTEKSSSGLVRSLSLVDITMVGIAAMIGGAIFILVGPGIHEAG
                     SALMIAFLLNGIITFFTALTYAELGSALPEAGGGYKWIRMGLPRPNAFISGWMAWFAH
                     MIAGSLYAVGFGAFLGHLLTMVGVSGALAGIALDKIIAVIVIVLFTYINVKGVSDTGR
                     VGNTITISQLVIILVLVVAGLYAMTFTNPDWPSNFRDFFPNGSIGLIIAMGLTFIAFE
                     GYEVIVQTGEEVKNPKKNIPKAIFISLGVVVTLYIVFTFAFIGGLNPDEIGKESWKFI
                     GDHGEIGIVEGAKFLLPFGALIVLAGALISSLSALNATTFSSSRVAFAMGRQYNLPVE
                     FSKIHTKYHTPHYATIASGFIMIILAVLLPLEQLALAAGVMFLFLFTQVNIAAITIRR
                     LYGHKLDYGFKIPFFPTVPLIGIFTKIGLAIYLLVYNPLSWLIAIIWIIIGFSLYRVY
                     SIKKELEHYAPLVASEGPQERKDYRIMVVFEQNNAEKLAKIGSAIAQDKQGEISFLNV
                     VVVPIQLPLSSAQGFAEPAMKAFDVIKKTLPQYEEHRYLVRLSHDNTEAILATVEEQG
                     INLLVMDFHSMRSNRKLLSLTTCDIIGVNVKKNFDNELSNLVVSYDKGRHSNLGLEIA
                     NSISKSLNSTIRIVRGVVESQEEERDVLSRINEMMFDLDLKKIPVERVYSKTENVTHD
                     LLENFEGNTPEIIIVGAGNQSDQAFSPKTMEIIEKSSKTVFVIRNSRFSGIQARYFWN
                     MIAPRLRENRFIYKLYLGAMRLVFFINSKRAKVRSDEDFFESKM"
     gene            complement(10076..10579)
                     /locus_tag="VLB45_05145"
     CDS             complement(10076..10579)
                     /locus_tag="VLB45_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007401329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF367 family protein"
                     /protein_id="HSD05122.1"
                     /translation="MQVHVLMFKQDDPKKCTAAKLVKFKLAKSVRTTPPNTLLLNPFA
                     EKTILKNDASQVDSITGIDCSWNLAEKMFKKKFVGIVRKLPPLLAGNPVNYSKIGKLT
                     TVEAIAGAAFILGFDDLGQQLLDKFKWGHTFYDLNRDLLLDYSKANSEQEIDLLVNEY
                     GLLGSSQ"
     gene            complement(10591..13809)
                     /locus_tag="VLB45_05150"
     CDS             complement(10591..13809)
                     /locus_tag="VLB45_05150"
                     /inference="COORDINATES: protein motif:HMM:NF013125.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HSD05123.1"
                     /translation="MKSHLMFGFFVVILLFVQAYPAMAQSGDHVVINEVDINPPGDDS
                     KSVAEWVELYNPTNQTMDVGGWTIGAASGLKTTYKVPVGTQLKSGGFLAYTFGPLWFP
                     DISAAVQLKDKNGTVIDQTPLLSDIENNLKSWQRTTDGLDTDSASDWIFKTSNAGSSN
                     GKTSSTATQSSLTITVLTDKTNYIFGEPVKITGQVSKRVNVPNQSYTPEEINIVISDS
                     SGFKRKIVMYPDRLLSFKSEFKTDRVFNIPEGGYKVSVEYAGATSEAEFSIGEKAFVA
                     PQKDASALITISTDKLQYVPGERATISADTSKIIPFAGMQFKVFDPSNKQVYDGTLYP
                     DTKGKFSVQIFISTVRPVFGIYNVVATYDKATTKTTYELVQDIKEDKAISLVTDKRAY
                     GLGDTVIITGRLNTVISPTLELEIVQPFVSKNVANTFVIKDLLRTKDFGGDGTFRYEF
                     KIPSNNERLGEYKVKVNAQSLDSTEVSFNVVENPDEFVDVISGPLSLTTDKPVYAVGD
                     TLVISGRVSETKDLGRQNVKISITDDSGKPIISKGDPGGSAGKNQDAVYSFTGIPDST
                     GQFEVKSSIYRNVFQKGTYLIKAVYGENTVSTSFSVVDSVDLGSGVKIIASTDKEIYG
                     IGDQVQLTGKVSTFTAQTSYQITITDPNGKQTRSSVTINNGQFSWGWTIPDYLQTFGI
                     YKLTVSSDSDKTDVFFKISQNPESDTILQPLMIETDKAVYTSGDTITVFGHAITKSSS
                     TTSGSIVNVRPIITIKTDANKVVYTSTPDLNTGGQFQSSFKVVPAVFKTGEYKVIAKY
                     YKETAQTVFKVDDTFGSGGDVPLVLLLETDRDQYLPGDTVKITGKTSKIVSVFEVDVT
                     VSKGGEAKSPIAIKFDPSGSFNYDYKIPQTNSLGIYTVKADTDFDTVVDKFEVVSELP
                     QETTEEPTGETPISIIPTKITDKVNRITGSSIPVLIEAKEMEDLTYTPRLFDGLLRVN
                     QGDESSVNIKLTFDGVCIIGPDSDCQVTKSTRDDSSLYQIIEIDGENLKVRYSGSGAK
                     LEKFTILPDDSDGMIPSGDWNVEIIKNKQISRFYYKISYTSQ"
     gene            complement(13978..14220)
                     /locus_tag="VLB45_05155"
     CDS             complement(13978..14220)
                     /locus_tag="VLB45_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010190714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05124.1"
                     /translation="MGFEKREIILIVGILFLMIGMMPFIPTIYAVSIAIIIYFGIRIF
                     VGRKKSQLQKSIGSGICAVCGEKIIKNRCPNCDKAE"
     gene            14457..14756
                     /locus_tag="VLB45_05160"
     CDS             14457..14756
                     /locus_tag="VLB45_05160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05125.1"
                     /translation="MGKRKFTIDLGSEKIETEGHMHKNVAIKYLMKRRRSLLMTRDKE
                     KVEKLYESVPKTISIVGGHLTKSYKVNWEREGTTEFEGSRFVFTLEELPDQEIAA"
BASE COUNT         4401 a         2812 c         2768 g         4826 t
ORIGIN      
        1 ccttatccac aaaagtaccc ttttccgcaa aagaggcaag cggactgcaa acaatcatga
       61 ccagcattgg caccagtaat ggtttcatga tgtagtttga tttggactgt ttttaatctt
      121 ctttggttat tttcatgtca atccaatgct tgcaaccaca ttgattttac gcattcaaaa
      181 tgtcacgatt aaaaaatata gttctaattt gtaccatttt ttaccattta atttcttaaa
      241 ctgtatataa tagaaaaata aatccagacc tgttgaatct tcaaaccctt gtaagaggaa
      301 taaatggaat aattcctggc ggcatatcag taaaagattt ttcagttgta acccagattg
      361 acaaagatac tgctgaaaaa gtattagaca atctaatgca aaatggaata ggcagatttg
      421 aaaatgattc catatatttt gaagaaaatg acaagctaaa gacatcgctt ttggcaataa
      481 gaatgggagc cgcagttgat gaaatatcgg aattgcttag ctggcaagat tttgaagcac
      541 tcgcagagga aattctaaga tcagataatt ttgatacaac aagaaacctg acacttacca
      601 agcccagaat ggaaatagac gtggtcggga taaaatcaga tgtggcaatc ttgattgatt
      661 gcaagcattg gaaaagatca agccaagctg cattagaaaa ggcagtcaaa aagcaaattg
      721 agcgtacaaa acaatatctt gtaaaagaaa aaatcagagc tgcagtccct gcaattgtga
      781 cattatatca agagcagatc aagttcatag acaaggttcc aatcattcct atattccagc
      841 ttggttcatt ttgtgaggaa ttttacggaa atctagacga gatgaacaag ttagaacatg
      901 gtcaggaaga aaaagacggt agtccctatc aaaaagacct gcgttagtct catcgtacga
      961 tctagtgatt tcgaataatc ttcaagttgt gtggtgccca tcactttgac atcagtttca
     1021 tgctgtagcg attcaaatga tgcaggagaa agagtctgtt ctgccttttt tgcaagatca
     1081 agataccaag atgcaacgtt ctcaaatgtc gatacgcttc caaaagtaaa gtatccatgt
     1141 ttgtttcttg cctcttttgt cttgtagtgg gtatcagagg aacacatttc gattaaagtg
     1201 taaccgtgtt ttgtaaagta acttaccact tcttcccgta atccattttg catgttattt
     1261 gaatcagacc agccaatgaa aaacttctca ccattcactt tcagacatag gattccaatt
     1321 ccacttggac caagatcatc tgccttgatt tcggtatttt ccaagtgtgc atatccagtt
     1381 tccaatggat atgattcttt tattttcaaa gtgtcaagat ttgatttggc tgctttgaaa
     1441 agatcatctg catcattttg agaaatctct tctcccattg cattgtgact atcaactatc
     1501 aagagttttt caaatccacg gttttttgcg tattgttcta gttcattttt tacataaagt
     1561 ggaacatctt ccatcccatg cggcgagaga gataagataa gaactgctgt cttgtcaaat
     1621 aacagtccga ctgttcttgt tttgtttacc tgtactgtta caggttttgt gcacatatta
     1681 ccttgattta ggacgtgact ttttgcaaag cttgctaaat atgcatcaac ttgtgatttc
     1741 gatggcagat taagagaatg atcagaaata ccatgcatca ccatagcaga cgaattaaga
     1801 ttcttgtata tcaaatatgg aatgttactt ccacctatgg gatgaaatgg tcctggatga
     1861 acatcaggca atacgagatg aaattcattg tctcttgttt tgaatttaat ttgagatgtt
     1921 gatacttttg atggttgtga gcgttcttcc attaggcgct ccatttctga agaatcgttt
     1981 cttgtccatg ctgcaatata cgcccgaaga acgttgtgcg tactaggaat tttttttcct
     2041 ccggctctgt ctgtcactat gctccaaact gttcccacaa ccaagaacac aactccgtag
     2101 cctattgctt gcaggtcagc aagcattggg atccaaaatt gagatggaat tattgccaga
     2161 aacattgcaa gaggctgtat cagacagatt gcccaagcta gtttgacttt tgcacctata
     2221 acagaagtaa agattcctat cctaaagctt gcaaacaaaa acattccttc agtcaagtat
     2281 attggcaata gatctggttt ggaaaacagc ggtacagaga ttataccacc caatgcagta
     2341 acaaaccaaa gaagactgcc aaaaccagag atgtgtctag ctttggattt ctctgtatgc
     2401 agcaaaatag aatcaagtat ttgtgttaca agcaacaccc ctatagtcaa aaacatgtga
     2461 aagaaaaact tgtcatagtt ttcaaaataa gcaagttcag caagtatcac caacaaagat
     2521 gcagccgcaa tagaaacaaa aagagatagg taaggtgatg agggggtaag aagagttagc
     2581 gaatatctcc tatgaatatt ggaaacgttt tctgcgtttt cagtcatgac aggaaaaata
     2641 ttttcaatat ccacgaaatt gcaattaatc cacttggcat ggccactact atctctggcc
     2701 ttattttgta tccttttgtc tcgtcctcaa agaacctcaa aagacctgcg cttgaggcag
     2761 gcagtggggc tgattttttg ctactcatgg ttactttgag agtcaggatt tttacataaa
     2821 aacttttgtt ctattttttg agtttttcaa ttattccaag cagttcgttt atcgatttta
     2881 ggatctcttg ttgtttttgg tgatcttttt catgttcaag ctcttctgta aggatctgta
     2941 atttttgttc acattttgca agtggcgtat tgatttctgt tttttccttg tctgctttta
     3001 ttggaatatt ttcttctttt gcttctcttc cacagtttac acaaagtgca ctaccatcac
     3061 gcattactct gactccttta cagtacggac aaggttcgct tgaaagagtc gcacctttaa
     3121 gaagcatttc cactgctttt tttgtaagat cttttgacac aaatctaatc tgcagatcca
     3181 agtaaaaaag atagatacgg acaaggctta atagtaggaa agcaaacttt tactttgaat
     3241 ggcggtaatc tgcaatacat gcggacttcc caacgattta tgcgcctgtg gtgaacttga
     3301 aaaagatgct actcaaattg ttgttcgtgt agaagaaagg cgttttaaaa agaaaggaac
     3361 gatgattgaa ggaataaatc caaagatgaa tgatcttgga aaggttgttc gagaattaaa
     3421 agcaaaatat gcatgtggtg gcacagtaaa agacggatac attctacttc aaggtgatca
     3481 cagagataca ataaagggaa cgctggtaaa gttgggattt cctgaagcga gtatcgaagt
     3541 tcactaggcc ttgctccaaa gcgataaact catcaaagct cttactactc cctattctgc
     3601 tttaatgtca gtaatctttg ggatgatgat tgtctcattt cccattggag catatgttgt
     3661 ttttaataca gatattggaa gggagataaa ttttgaatat ccacttgacg gaatcaatgt
     3721 ttttcttggt ggaataggtt tcaaaatccc gttccagttc gagcttggag acggattcat
     3781 tgttgcatgg tctgcatatt tgattttatt ttccatatca atttgcggac caaacaagaa
     3841 tttcatcaaa tctgtttcag atatcatgtc aggcggttgg gaaaatatca aaaacaatta
     3901 tcttttgagc atgctcacct ggttttccat tttggtagtg ttttctatta taatagattc
     3961 attgcaacaa agtgtaggaa tacctataga acctccagaa ttttcaaatg gattaatcca
     4021 gtttttccag attacagttt ctcctctgac tgaagagata ggatttagga tgtcgctaat
     4081 tggtcttccc ctttttgcaa tgtttgcacg taaggcatcg tttacccatt ttttaaaatc
     4141 attatggcat cctgcaaaaa atcttgaaat cacaaattac aaaaaggcaa taggtttggt
     4201 cataatagtt gggatatttt ttggagtgtc acacataatt tctggcacgc catggagtgt
     4261 aggaaaggta gctcaagcat cgtttggggg agtgataatt ggatgggtgt acatgagata
     4321 cggtttggcg cctgcaatct tgttgcactg ggcaacaaac tattttgtat tctcgtatgt
     4381 gttttttgtt tcaaacataa atcagacatc tattacagat ggatttacaa atccatttct
     4441 tggtactgtt gaaattttgc ttatcattgc aggaatatta gcagctactc tgcttgtatt
     4501 agattattta agaacaaaaa aagaatctac agttactaca gtatgatcac tgtatcagat
     4561 cagaagcaat cttggaaaat gactgtgaat attttgaaac atattttaga tcattaagat
     4621 catctacgtc aagcattatt cgtcgtatca gcacaagtgc agaagatgca ccatgttttt
     4681 ctgcagtgtt caagtgtatc ttatagctgt cttcgtcata gtgtgtttcc ataagatttg
     4741 ttggggttct aaacagtgcg tttgtgccat caaattttct agatggaact attagcgcac
     4801 aagggttaga taattgaaaa ttaaacagtg tttgtatgtc ctcggcttgc attagtggta
     4861 tgtcttgagg aaatacaatg gttgcatcaa gtccttcttc tgaaaaaaac ttgtctgcaa
     4921 gagacactgc attgttaact cctagttctt tttcatcaag aatttccaca catccgaatt
     4981 tttttccaat ctcaaacaca ttttcatctt ttgttacaag cgcaattttc tctataacag
     5041 aagagtgcga gattgtatct agtacttcat tgagcattag tctacagaga atttctattt
     5101 tttctggtgg tagattgagc cgttttttgg ctttggaaaa agttttaaca ggaacaatcg
     5161 cgcctgttct aaatttagac atgcacttgt tttagaataa acgaagcaag agcatcttct
     5221 tcaattttgt ctttcatttt tattttggtt tcatacacgt gcatatccaa gttctggatc
     5281 tttcttgtaa gcagacggtc agatgaatcg attatcatgt gtgatgcgac ttcagaatac
     5341 atttttgcca gtccatatgc ggaaacttcc attccagctg cctccatgta ttttgcagca
     5401 ggtccactga ttgctttatt tcccatcagc ggactgacaa caacaacttt cttttttgat
     5461 tttgataatt ctttttttat tcctttgata gaaagaattg gtcctatact tgttagtgga
     5521 tttcctggag caattatcac tagcttagaa tcatgaattg cgttgaccgc tgcaggattt
     5581 gcgcgtgcct tgtctgctcc aagatatttt attccctcaa cattatcttg ccctcggtac
     5641 tttacccagt attcttggag gtgcagttct cccttgtttg taactattct tgtttctaca
     5701 gtgttgtcag ttactggaat tatctttgtg tcaattgcaa atttttggca catccaagcc
     5761 gttatgtcag tcagattttt gccgtttttt agcatgtttg ttctcaaaag gtgtgtggcg
     5821 acgtctctgt ccccaatctt aaaccatgtt tcctctccaa gtatttccat ctggcgtaaa
     5881 aatccaaacg tgtcttttct aattccccat cccttgtcaa tgtcaagcaa gtcagcaagt
     5941 ccgtaaagga tggtatcaat atcaggacaa acatacagac cataaagcca atagttgtct
     6001 ccaacgtttg agattacact aatgtccctt gtttgtgaag atactccacg tacaagtttt
     6061 accgaaccag tgccaccagc taatattgta atcatttttt caactcttaa tacgacctca
     6121 ttatgaagat aaatgtacaa taattacttt tgccaagaca gtctttgatt agaaaattta
     6181 tcatttaaaa aaagaataag atttattact atcagctaaa gcagttttta cgtgtcaaaa
     6241 agcataattt tttctgcagg attaattgga tttttgcttg ttggaactat ctctccgatt
     6301 tgggcagcac agattgatgt gagattaagc acacatttag aatcagcaca gcccactttc
     6361 aaatttttaa aaactgtttt catagaatat cctgacggag gagaactttc aagaacttta
     6421 cttggtaaag acattctagt cactcttgaa gctgataaaa gtagcccggg agtttctgaa
     6481 ttgataaata aaataaattc caatttgaaa gaattagaca gcactgtctt tgtcactgac
     6541 cttactatga cttatagagc acacctagtt ggaagaggag accaaacagc aatagattac
     6601 aaagttacac tagagccaac aatcgtaaat tatattttaa gacaagcaac tgaatcatcc
     6661 ccagctctaa ttgatgcaca atggaggggg attaccataa cagggcccat cacaattaca
     6721 gatgctgaac ttggagatgt tgaagtaaat acactcatag gtttcataca gaaagaagtt
     6781 ccagaagcat acgacatcat atctggaact gaagctgaaa ctctattgaa tgcaaacctt
     6841 atcgatgcta gtggagtatt aaaacaacca cttgccaact ggcaccatct atttgatcca
     6901 tcaggtacaa tttctgacac tgccaagtgg ggttatcaag gtgaaaaagt tgtgataaca
     6961 agtttcacca tgggagaaag cagcttaaga gaaggaatgc aaaaagaacg tgaacactct
     7021 gcagagttta caacggataa aaaatatgtt gttagaactg tagaatcagc tgattctgca
     7081 aacatacaga tcgatggatt tgtaacagaa acttcgatag aaggtgcaga atattttggt
     7141 tctagtccaa aagttccaga aggatatgcg actacatcaa ctggaggatt tccagtgggt
     7201 gttatgtatg gcatggcagg cgcaggagct gcagttgcag tttttgtcct agtctggagc
     7261 gatagaaaat taaagaaaca aaatagacaa taattaaaaa agtggcgcgt gccagccgta
     7321 accctccttc tgtttttacg agattcagct atgcggccta cctaaacaaa gtgcagcact
     7381 tgtagttttg tttggccttg ctccgtgtgg gtcagctctt ttcattcttt gccggaaacc
     7441 tcaggcttta ccatcacagt acgccggttc agatagtttt ctgttctgga gccagtcatc
     7501 tctgactatc catctctgga tcacacttac tgcatggagg gaggagtttc ctctgcctta
     7561 gcagtagctc atgcaccagc tcgcacctaa agtagagtga gaaaaatttc tattttaaca
     7621 ttgttagagt ttcaaagcat aaaaactaca ttttagattc aaagaaatct tcatcagagc
     7681 gtacctttgc tcttttagaa ttgataaaaa ataccaagcg catagcaccg aggtatagtt
     7741 tgtagataaa cctattttct cttagtcttg gagcaatcat attccaaaag tatctagcct
     7801 gtattcctga gaatctagaa tttcttatga caaagacagt ttttgaagat ttttcaatta
     7861 tttccatggt ttttggacta aacgcttgat ctgactgatt cccagctcct actattatta
     7921 tttctggcgt attaccttca aaattttcaa gcagatcatg tgtcacattc tcagttttcg
     7981 aataaactct ttcgacgggt atttttttca gatctaaatc aaacatcatt tcgttaattc
     8041 tacttaaaac atcacgttct tcttcttgag attctacaac gcctctaact attcttattg
     8101 tgctgtttaa tgatttggaa atagagtttg caatttccaa tcccaggtta gagtgtcttc
     8161 ctttgtcata tgataccacc aagtttgaaa gctcgttatc aaaattcttt ttgacattaa
     8221 caccaataat atcacatgtt gtaagtgaaa gcagttttct attactacgc atgctatgaa
     8281 aatccattac gagcagattg attccctgtt cttcgactgt agcaagtatg gcttcggtat
     8341 tatcatgaga aagtctaaca agatatctat gttcttcata ttgtggcaat gttttcttta
     8401 tcacgtcaaa tgctttcatt gcaggttctg caaatccttg tgcagaagac aaagggagtt
     8461 gtattggaac tacaacaaca ttaagaaatg aaatttcccc ctgtttgtct tgcgctatgg
     8521 cagaacctat ttttgctagc ttttctgcat tgttctgttc aaagacgacc attatgcggt
     8581 agtcttttcg ttcttgcgga ccctcacttg ctactagagg tgcataatgt tctagctcct
     8641 ttttaataga atatacacga taaagtgaaa aaccgattat tatccatatt atggcaatta
     8701 accagctaag agggttgtag acaagaaggt atatggcaag gccaatttta gtaaatattc
     8761 caatcaatgg tactgttgga aagaaaggga tcttgaaacc ataatccagc ttatgaccat
     8821 ataatctgcg tatggttatt gcagctatgt tgacctgcgt aaaaagaaag aggaacatta
     8881 caccagcagc cagtgctagc tgttcaagtg gcaacaatac tgctaaaata atcatgataa
     8941 atccagaagc aattgttgca taatgcggtg tgtggtattt ggtgtgaatt ttactaaatt
     9001 caacaggaag gttgtattgt cttcccattg caaatgcaac tctgcttgat gaaaatgttg
     9061 ttgcatttaa tgcagagaga ctagatatga gagcgcccgc aagaactatc agagctccaa
     9121 acggtaaaag aaattttgca ccttctacta taccaatttc tccatgatca ccaatgaatt
     9181 tccacgattc ttttccaatc tcatctggat ttaggccacc aatgaaagca aatgtaaaaa
     9241 ctatgtacaa agtcacaacc actcctaatg aaataaaaat tgcttttgga atgttctttt
     9301 ttgggttttt tacttcttca ccagtctgaa caattacttc ataaccctca aatgctatga
     9361 atgtcagtcc catagcaatt atcaatccga tactgccatt tgggaaaaaa tctctaaagt
     9421 ttgaaggcca atcaggattt gtaaatgtca tagcataaag accagctacg acaagtacga
     9481 gtattataac aagctgtgag atggtgattg tgtttcctac tctacccgta tctgaaactc
     9541 ctttgacatt tatgtaagta aaaagcacaa ttacaataac agcaataatc ttatcaagtg
     9601 caattccagc taatgcacca gatactccta ccatggttaa cagatgacca agaaaagcac
     9661 caaaaccaac agcatagaga cttcctgcta tcatgtgggc aaaccacgcc atccatccac
     9721 tgataaacgc atttggtctt gggagtccca tccttatcca cttgtatccc cctccagctt
     9781 ctggcagtgc tgacccaagc tctgcatacg tcagtgctgt aaagaatgtg attattccgt
     9841 tgagtaagaa cgcgatcatt aaggctgatc ccgcttcatg aatgccaggt cctaccagaa
     9901 taaagatggc gccacctatc attgctgcaa tacctaccat cgtgatatca actagtgaga
     9961 ggctacgtac tagaccagag gatgactttt cagtagacat tattacctat ccgtgtggaa
    10021 aaaatggtcg aataaatctc tactcgttga ccggactcta aacatggaga aatatctatt
    10081 gactcgaacc taaaagtccg tattcattta cgagtagatc aatttcctgt tcggaatttg
    10141 cctttgaata atccagaaga agatccctat tcagatcgta aaaagtgtga ccccacttaa
    10201 atttgtcaag tagttgttgt ccgagatcat caaatcccaa tatgaatgcg gcacctgcaa
    10261 ttgcctcaac tgtagtaagc ttgccaatct ttgaataatt tactgggttt cctgctaaaa
    10321 gagggggcaa ttttcttaca atgccaacaa attttttttt aaacattttt tcagcgaggt
    10381 tccatgaaca atcaattcca gttattgaat caacttgtga tgcatcattt tttaaaatag
    10441 ttttttctgc aaaaggattc aaaagcaacg tgtttggagg agtggttctt acactttttg
    10501 caagcttgaa tttgacaagc tttgcagcgg tacatttttt tggatcatct tgcttgaaca
    10561 tcaacacatg cacttgcatg gtgtgtgtag ctattgtgag gtatatgaaa tcttgtagta
    10621 gaacctagaa atctgtttgt tttttattat ttccacgttc cagtccccgc taggaatcat
    10681 tccgtctgag tcatctggaa gaatggtgaa cttttcaagt tttgcgccag agccgctgta
    10741 tcttacctta aggttttcac cgtctatttc tattatttgg tataacgaac tgtcatcacg
    10801 tgttgatttt gtaacttgac aatcagaatc aggacctatt atacaaactc catcaaaggt
    10861 tagctttatg tttacagaag attcatcgcc ctgatttaca cgcaataacc catcaaagag
    10921 tcttggagtg taagttagat cttccatttc ttttgcttca attagtacag gaatgctaga
    10981 gcctgttatc ctgtttactt tgtcagtgat ttttgtagga atgatagaaa ttggagtttc
    11041 gcctgtaggt tcttcagtag tttcttgcgg tagctcactt accacttcaa atttatctac
    11101 aactgtatca aagtcggtat cagcttttac tgtataaatc ccaagactgt ttgtttgtgg
    11161 gattttataa tcatagttga aagaaccact tggatcaaac tttattgcga ttggcgattt
    11221 ggcttctcct ccttttgata ctgtgacatc cacttcaaag acagaaacga tcttactagt
    11281 ctttccagtt atctttacag tatctccagg gagatattga tccctatctg tttccaaaag
    11341 taaaacaagt ggtacatctc ctccactacc aaaggtatca tcaactttga aaacggtttg
    11401 agctgtttcc ttgtagtatt ttgcaatcac tttgtattct ccagttttga aaactgctgg
    11461 aactacctta aacgaagatt ggaattgtcc tccggtgttc agatctggag ttgaagtata
    11521 aactaccttg ttggcatctg tttttattgt aattataggt cttacattta ctatggatcc
    11581 tgaagtggta ctagaagatt tggttattgc atgaccaaac actgtaattg tatcgccgct
    11641 tgtatagact gccttgtctg tttctatcat aagtggttgc aatatagtat cagattctgg
    11701 attctgtgag atcttaaaga agacgtctgt tttatcagaa tcagagctca cagtcagctt
    11761 gtagattcca aatgtttgca ggtagtctgg gatggtccag ccccaagaaa actgaccgtt
    11821 gtttatagta acactgctgc gagtttgctt tccatttgga tctgttatag ttatttgata
    11881 agatgtctgt gcagtaaatg tagagacttt gcctgttagt tggacttgat cccctattcc
    11941 gtatatttcc ttgtccgtac tggcgattat ttttacgcca gatccaagat caactgaatc
    12001 cactacagaa aatgatgtgg aaacagtgtt ttcaccatat actgccttga taagataggt
    12061 tcccttttgg aagacgtttc tgtatatact gctttttacc tcaaattgtc ctgtactgtc
    12121 aggaattcca gtaaatgaat atacagcatc ttggttttta ccagcagagc cacctggatc
    12181 accttttgat attattggtt ttccgctgtc gtcagtaatt gagattttga cattttgcct
    12241 tccaagatct ttcgtctctg acactctacc tgagataact agcgtgtcac caactgcata
    12301 gactggtttg tcagttgtaa gagaaagcgg tcctgagatc acatcaacga attcatctgg
    12361 gttttctacc acattaaaag aaacttcagt gctatcaaga gattgagcgt tcactttgac
    12421 tttatattcg cctaatcttt cattattgct tggaatctta aactcgtacc tgaatgtgcc
    12481 atcaccacca aagtcttttg ttctaagcag atcttttatt acaaaagtat ttgcaacatt
    12541 cttgcttaca aatggttgga ctatttctag ctcaagtgtt ggagatatga ctgtgttaag
    12601 tcgtcctgta attattacgg tatcgcctag tccatatgct cttttgtccg ttactagtga
    12661 aattgcctta tcttctttaa tatcttgtac caattcgtat gttgtttttg tggttgcctt
    12721 gtcgtatgtt gccactacat tgtagattcc aaagactggt ctaactgtac taatgaaaat
    12781 ttggactgag aactttcctt ttgtgtcagg gtatagggtt ccatcataca cttgcttgtt
    12841 acttggatca aataccttga attgcattcc agcaaatgga ataatttttg atgtgtccgc
    12901 actaattgta gctcgttctc ctggaacata ttgtaatttg tctgtactaa ttgtgattaa
    12961 agcggatgca tctttttgag gtgcaacaaa cgccttttct cctattgaaa attctgcctc
    13021 tgatgtggca cctgcatatt ccacagatac tttgtagcca ccttctggga tattgaatac
    13081 cctgtcggtt ttaaactcag atttgaatga caatagccta tcaggataca taactatttt
    13141 tcttttaaat ccagatgaat cagaaattac aatattaatc tcttcaggag tgtatgattg
    13201 atttggtacg ttgactcttt ttgaaacctg gccagttatc tttactggct cgccaaaaat
    13261 gtagttggtc ttgtcagtta acacagtgat agtcagagaa gattgtgttg cagttgacga
    13321 agtcttgccg tttgatgatc ctgcgtttga tgttttgaat atccagtcac ttgcagaatc
    13381 tgtatcaagt ccatcagttg ttctctgcca tgatttcaag ttgttttcta tatcactaag
    13441 aagtggagtt tggtcaatca ccgttccatt tttgtctttt agttgaacgg cagctgaaat
    13501 atctgggaac cataaaggtc caaacgtgta agctaaaaat ccaccgcttt tcagttgcgt
    13561 acctacaggt actttgtagg tggtcttaag accagatgct gcaccaattg tccatccacc
    13621 gacatccatt gtttgatttg tcggattgta cagctccacc cattctgcca cagacttgga
    13681 atcatcgcct gggggattga tatcaacttc attaattacc acatggtcgc cagactgggc
    13741 cattgccgga tacgcttgta caaatagcaa aattactacg aaaaatccaa acattagatg
    13801 tgacttcaat tctcttacct gtgttaacat tccatcacga ataatgcaat tttaggtgtt
    13861 gattgtggaa aagttgagct agttgtgaat gccaaatatg catgtgcgta catgtgtaaa
    13921 agccggtttt atgcattata agcagtgcgt aggatttatt ctacagtgag aatcgtttca
    13981 ttccgctttg tcacagttgg gacagcggtt tttaattatt ttttcaccac agactgcaca
    14041 tataccagag ccgatggatt tttgcaattg actttttttc ctgcccacaa aaatgcgaat
    14101 gccaaaataa atgataattg caatactaac agcatagatt gtaggaataa atggcatcat
    14161 tccaatcatt aaaaataaaa ttccgacgat cagaattatt tcacgttttt caaaacccaa
    14221 ttttgtagat ggtgaaaagt tttaataaaa atctatgcca gtgctcagag tcactgaatt
    14281 tgcattcatc ttaaatcatg tctctaactc ttcttcatcg cctggccata ttagaatcgc
    14341 aaaagtgaga taagaaccat ctgaatgtac atacaaaaat agtcattatt tttactcttt
    14401 ttacttggca tccgttggtc tgcatccttt agtagagttt tgtatgacat ggttttatgg
    14461 gcaagcgaaa gtttactatc gacttgggca gtgaaaaaat cgaaaccgag ggacacatgc
    14521 acaaaaatgt agccataaaa tatcttatga aacgacgcag gtcactttta atgacacgcg
    14581 ataaagaaaa ggttgaaaaa ctatacgaaa gtgttccaaa aaccatatcc atcgtaggtg
    14641 gacatttgac aaaatcctat aaagtaaact gggagagaga aggcacgaca gagtttgaag
    14701 gatccaggtt tgtcttcaca ttagaggaac taccagatca agaaatagct gcttagcatt
    14761 ctaagaaaat tcgtctcatc attacaatgg ttaaatgata tacaatc
//
LOCUS       DATIDL010000116         5041 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrosopumilaceae archaeon isolate SMAG_U16342
            k141_1156877, whole genome shotgun sequence.
ACCESSION   DATIDL010000116 DATIDL010000000
VERSION     DATIDL010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935836
            Sequence Read Archive: SRR25158326
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosopumilaceae archaeon (soil metagenome)
  ORGANISM  Nitrosopumilaceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrosopumilales;
            Nitrosopumilaceae.
REFERENCE   1  (bases 1 to 5041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/04/2024 05:09:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,520
            CDSs (total)                 :: 1,474
            Genes (coding)               :: 1,474
            CDSs (with protein)          :: 1,474
            Genes (RNA)                  :: 46
            rRNAs                        :: 1 (5S)
            complete rRNAs               :: 1 (5S)
            tRNAs                        :: 43
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5041
                     /organism="Nitrosopumilaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1156877"
                     /isolate="SMAG_U16342"
                     /isolation_source="soil"
                     /db_xref="taxon:2202732"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..534
                     /locus_tag="VLB45_05165"
     CDS             <1..534
                     /locus_tag="VLB45_05165"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HSD05126.1"
                     /translation="IRENRRHRPPRAFEMTEYTFDLLTNFGMFRDFHRHRVLTLERQL
                     LTTNHGFSISSEIHNLGIEKDYLDCMYKSKEVFDLIKPKMPEQAQYVVNFAYNYPYFM
                     RLNLREATHLIELRTVPQGHPDYRKVGQNMFRAIKKAHPVLSQIIKFVDLKEYKLERL
                     ESEKRIEEKRKKVIPAD"
     gene            650..1015
                     /locus_tag="VLB45_05170"
     CDS             650..1015
                     /locus_tag="VLB45_05170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05127.1"
                     /translation="MEIKEVRDKVLEVGNITSCAVMVIEKKGDILEYVITDPTKTIVS
                     LSDLVELAGVMALRYDVVGYDKMLGGLIMTTDEFRDHLTISSMIKENILVVVIPNTAD
                     LNKVTAEIKNIVAIKLKNS"
     gene            complement(1012..2037)
                     /gene="fen"
                     /locus_tag="VLB45_05175"
     CDS             complement(1012..2037)
                     /gene="fen"
                     /locus_tag="VLB45_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007550252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease-1"
                     /protein_id="HSD05128.1"
                     /translation="MGLDLKPLVIREKTNLESFASKVVAIDAYNAIYQFLSIIRGPEG
                     LPLADSKGRVTSHISGLFYRNINFLSLGIQPVYVFDGKPPSLKSAEIERRKQIKKDAT
                     IKFEKALSEGKYEDAKKYAQQTSSMRDEMVEDSKHLLNLFGIPFIQAPSEGEATAANL
                     TITGQAYASASQDFDSLLFGAKKLVRNFTNSGRRKLPNRNTTIEIEPEIIDLEKTLTD
                     LGISREQLVDIGILIGTDFNPNGFERIGPKTALKAIKEYSKLENVPQIQEKLEEIDYK
                     QIRQIFLEPKVASVSKIEFGETNFQGIINYLSSERNFSLDRIETSLNRLKKAKEKKSH
                     TLEQWFS"
     gene            complement(2072..2254)
                     /locus_tag="VLB45_05180"
     CDS             complement(2072..2254)
                     /locus_tag="VLB45_05180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05129.1"
                     /translation="MPKFRVSITIDSRIAKEIEDYYREKVKTAAEKGEDIPKLSNIYE
                     EIIERGWEAKSGLRKK"
     gene            complement(2330..2497)
                     /locus_tag="VLB45_05185"
     CDS             complement(2330..2497)
                     /locus_tag="VLB45_05185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05130.1"
                     /translation="METLTKKVVSIEFEGKKYVLPDELTLESFLSSLGFDDNDLVLIR
                     PTKDGLSLMLK"
     gene            2596..2690
                     /locus_tag="VLB45_05190"
     tRNA            join(2596..2631,2645..2690)
                     /locus_tag="VLB45_05190"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2628..2630,aa:Ser,seq:cga)
     gene            complement(2774..4267)
                     /locus_tag="VLB45_05195"
     CDS             complement(2774..4267)
                     /locus_tag="VLB45_05195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05131.1"
                     /translation="MTSITQIGTILAITAMIASSQAMLMGSAMATVVPSEVNEAAQAG
                     DEFIINKEVTTPTIPPKLDLLFLEDESGSFIDDIATIQGAAVDCSDGLAAEIWDGISA
                     AGVDDLNAGVAGFVDFAIFPYGAAGDHVYRLVQDMTADRTTWLNGICGLSTKNGVDFP
                     EAQLPALHTAAVGTAWDSNNDGDTIDPEDTPAGSDPTWRADTDVTRVVVLVTDADFHD
                     SAVEPAYPGTSFADTVSALNGEGIHVIVLTAGAVVVYDPLADATGGSVKPISSDSSDI
                     VSAIIDALEELETDVWFEIDPASCAPELDVSLVPEVHLGIPGDTTVAFTETITIPDGT
                     IGGEYLCEVDFFANTFGPDGDGVNIGTETILIDVNNPPDCDDAAASIASLWPPNHQMH
                     EITIEGVTDPDGDAVTLTISSIFQDEPTDGLGDGDVSPDASGVGTDTAQIRAERSGLE
                     DGRVYHVAFNADDGQGGICSSTVTVGVPHDKNDVPVDGGSIYDSTLP"
     gene            complement(4443..4748)
                     /locus_tag="VLB45_05200"
     CDS             complement(4443..4748)
                     /locus_tag="VLB45_05200"
                     /inference="COORDINATES: protein motif:HMM:NF026297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HSD05132.1"
                     /translation="MCAILEISSKIVKREFRANIEVLSRIATAFSQNSSMKKTHLHLA
                     TRISWTSFMRYLEWLQSKNYMISKMDGKEEKYELTETGREMFGMLLQFHDHVRFAEQ"
BASE COUNT         1470 a          973 c          900 g         1698 t
ORIGIN      
        1 atacgtgaaa acagacgaca tcgtcctccc agggcgtttg aaatgacgga atacactttt
       61 gatctgctga caaactttgg aatgtttcgt gatttccata gacaccgtgt tcttacttta
      121 gaaaggcagc tgcttacaac aaatcatgga ttttctattt cttctgaaat tcataatctt
      181 ggaatagaaa aagattatct tgattgtatg tataagtcaa aagaagtctt tgatctaatc
      241 aaaccaaaga tgccagagca agctcagtat gttgtaaact ttgcttacaa ctatccatac
      301 tttatgcgat taaatctgcg cgaagcaaca cacctgattg agcttagaac agtaccacaa
      361 ggacacccag attacagaaa agttggtcag aacatgttta gagcaataaa gaaagctcat
      421 cctgttctaa gccagataat caagtttgta gacttaaaag aatacaagct tgaacgacta
      481 gaatctgaga aaagaataga agaaaagcgc aaaaaagtaa taccggcaga ctagacatgc
      541 ctagacagtt tttttcttta ctatctcatg atataatgca agattgctga aaattgttaa
      601 cgattttaca ctgctccatt attattagga aattttctct atagaataca tggagattaa
      661 agaggtaagg gacaaagttt tggaagtagg caacataaca agttgtgctg taatggttat
      721 tgagaaaaaa ggagacattt tggaatatgt cataactgat ccaactaaaa ctatagtcag
      781 tctttctgat ttggttgagc ttgcaggagt aatggcatta cgttatgatg ttgtaggata
      841 tgataagatg ttagggggtt tgataatgac tactgacgaa tttagagatc atcttaccat
      901 ttctagcatg ataaaagaaa acatcctagt tgtcgtaata ccaaacactg ctgatttaaa
      961 caaagttaca gccgaaatca aaaacattgt agcaataaaa ctgaaaaaca gttagctaaa
     1021 ccactgttct agtgtatgac tttttttctc ttttgctttt ttcaatctat ttagcgaagt
     1081 ttcaattcta tcaagtgaaa agtttctttc agaggacaag taatttataa ttccttgaaa
     1141 attagtttct ccaaattcaa tttttgaaac tgatgctacc tttggctcca aaaatatctg
     1201 tctgatttgt ttgtaatcta tttcttctag tttttcttga atttgtggta cattttcgag
     1261 tttgctatat tcttttattg ctttgagtgc agtctttgga ccaattcttt caaaaccatt
     1321 aggattaaag tcagtgccta tcaaaattcc aatatctaca agttgctcac gtgaaattcc
     1381 aagatctgtc aaggtttttt ccaagtcgat tatttcaggt tcaatttcta tcgtggtatt
     1441 tctatttgga agctttcttc taccactgtt tgtaaagttt ctcacgagtt tttttgcacc
     1501 aaaaagaaga gagtcaaaat cctgactcgc tgacgcatat gcttgtcctg ttatagtgag
     1561 gtttgcagca gttgcctcac cttctgatgg cgcttggata aatggaattc caaatagatt
     1621 taacaggtgt tttgagtctt caaccatttc atcgcgcatg gaagacgtct gttgcgcata
     1681 tttttttgca tcctcgtatt ttccttcaga taaggccttt tcaaatttta ttgtagcatc
     1741 ttttttgatt tgcttgcgac gttcaatttc tgcagatttt agtgacggag gttttccatc
     1801 aaaaacataa acaggttgga ttccgagtga aagaaaattt atgttcctat aaaacaatcc
     1861 gctgatgtga ctagttactc ttccttttga atcagccaga ggtaatcctt caggaccacg
     1921 gattattgat aaaaactggt aaattgcatt atacgcatct attgctacca ctttagatgc
     1981 aaatgattca aggtttgttt tttcacgaat tacaagaggc tttagatcta ggcccataaa
     2041 atgttgtaat gattagatga tttttgcttt cctatttctt gcgaagaccg gattttgctt
     2101 cccatccacg ttctattatt tcttcataaa tgtttgaaag tttagggatg tcctctcctt
     2161 tttcagctgc agtcttgacc ttttctctgt aataatcttc gatttctttt gctatcctgc
     2221 tgtcaattgt tatggagact cgaaattttg gcatctttta cttgtcacct tgtgtgcgat
     2281 attaagaagt atgatatcaa taataaacta tccactatca attcctacac tattttagca
     2341 tcagtgaaag accatcttta gtaggtctta tgagtacaag atcgttgtca tcaaatccca
     2401 agcttgacag aaagctttca agagttaatt cgtctggaag tacgtatttt ttgccctcaa
     2461 attcaattga gacaactttc ttagttaggg tctccactga tctttgtaat aaatagtcta
     2521 ctttatatcc tttattatat atttgatcac tttcattctt agtgtattta ttgatcaaag
     2581 tatcaaattg cagttgccag gttagccaag cggtacggcg accgcctcga gatctaaaca
     2641 aggtagcggt tgtgcttctg cactcagggg ttcgaatccc ttacctggcg ttctgatttt
     2701 tttcttttat aatatactga catgtcatct aagagacata aatttggtat aattaattat
     2761 tttaaaaaaa gagttagggg agtgtggaat catatattga tccaccatcc actggtacgt
     2821 catttttgtc atgaggaact cctacggtaa ctgtgctgct acatattcca ccctgtccat
     2881 catcagcatt gaatgcaacg tgatagacac gaccatcttc cagtccggat cgttctgctc
     2941 ttatctgggc tgtgtcagtt ccaacaccac ttgcatctgg tgagacatca ccatctccaa
     3001 gaccatctgt tggctcatct tggaatatgc tgcttatggt aagtgttact gcatcaccat
     3061 caggatctgt tactccttca attgtgatct catgcatttg gtgatttggt ggccaaagtg
     3121 atgctatgct tgcagctgcg tcatcacagt caggcggatt gttcacatca attaagattg
     3181 tttcagttcc aatattgacg ccgtctccat caggaccaaa tgtatttgca aagaagtcta
     3241 cttcacatag atattctccg ccgatggttc catcaggtat cgtgatggtt tctgtaaagg
     3301 ccactgtggt atctcctggg atgccaaggt gaacttctgg gaccaaacta acatcaagtt
     3361 ccggtgcgca tgaagctgga tctatttcaa accagacatc agtttctagc tcttcaagtg
     3421 catctataat agcacttaca atgtcagagc tgtctgaaga tattggtttg acacttccgc
     3481 cagttgcatc agctagtgga tcatatacta ccactgcacc agcagtcaat acaataacat
     3541 ggattccttc accgtttaat gcagatactg tatctgcaaa tgatgttcca ggatatgctg
     3601 gttccactgc agaatcatga aaatctgcat ctgttaccaa gactacgact cttgtaacat
     3661 cagtgtctgc tctccatgtt ggatctgagc ctgcgggggt gtcttcagga tcaatggtgt
     3721 caccgtcgtt attgctatcc catgcggttc caactgcagc cgtgtgcaat gctggtagtt
     3781 gggcctctgg aaaatcaaca ccattttttg tgctcaagcc acatattcca tttagccatg
     3841 tggttctgtc agcagtcatg tcttgcacca atctgtatac atgatcacct gctgcaccat
     3901 atggaaatat ggcaaagtca acaaaacctg caactccggc atttagatca tctacacctg
     3961 ccgcactgat tccgtcccag atctctgcag caagaccatc agaacaatca actgctgctc
     4021 cttgtattgt tgcaatgtcg tcaatgaaac ttccactctc atcttctagg aataagagat
     4081 caagttttgg cggaatagtt ggagtagtaa cttctttgtt gatgataaat tcgtctcctg
     4141 cctgtgccgc ttcgtttacc tcacttggaa ctaccgtcgc cattgcactt cccataagca
     4201 ttgcttggga cgatgcaatc attgctgtaa ttgctaggat agttccaatt tgcgttatac
     4261 tagtcattac attcccaatt ttggtaatta ctaataagat tccctcactt gcagtaccta
     4321 cctgaagtga gcaatactta taaaaagaat aaaaatttgt aacaaaaaat ttccatatga
     4381 aaccaaataa atgaattttt gttttatttt acaaaattaa ttttgctaag atagaataaa
     4441 aattactgtt cagcgaatct aacatgatca tggaattgta agagcatacc aaacatttct
     4501 cttccagttt ctgtaagttc atatttctct tctttgccat ccattttaga gatcatataa
     4561 tttttggatt gtaaccattc caaatatctc ataaatgaag tccagcttat tctagtggca
     4621 agatgtaagt gtgttttttt catagaagag ttttgtgaaa atgcagttgc aatacgacta
     4681 agaacttcaa tattagctct aaattctcgt tttactatct tagatgatat ctcaagtatt
     4741 gcacacatct cacatgtaac caactattcg cttattttgc tttaaaatgt tttgcacggt
     4801 ttctattatt acattaatga ctaatcaaaa attagaaaat ttgtataatt cttgtattcc
     4861 catatagcct ataagactgt agtgagtatt tcatatcata catttgacta tatcattgtg
     4921 aaaatgcggg attagattct aaaaaatcta tgcatatttt gggttgacgc cacttgacgc
     4981 atcttgacac aatcatagtt cagatacatg atacaggatt cgaatgtcat cgtatgattg
     5041 t
//
LOCUS       DATIDL010000117         2157 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrosopumilaceae archaeon isolate SMAG_U16342
            k141_2979174, whole genome shotgun sequence.
ACCESSION   DATIDL010000117 DATIDL010000000
VERSION     DATIDL010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935836
            Sequence Read Archive: SRR25158326
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosopumilaceae archaeon (soil metagenome)
  ORGANISM  Nitrosopumilaceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrosopumilales;
            Nitrosopumilaceae.
REFERENCE   1  (bases 1 to 2157)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2157)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/04/2024 05:09:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,520
            CDSs (total)                 :: 1,474
            Genes (coding)               :: 1,474
            CDSs (with protein)          :: 1,474
            Genes (RNA)                  :: 46
            rRNAs                        :: 1 (5S)
            complete rRNAs               :: 1 (5S)
            tRNAs                        :: 43
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2157
                     /organism="Nitrosopumilaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2979174"
                     /isolate="SMAG_U16342"
                     /isolation_source="soil"
                     /db_xref="taxon:2202732"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..301
                     /locus_tag="VLB45_05205"
     CDS             <1..301
                     /locus_tag="VLB45_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007550518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05133.1"
                     /translation="KGSDHGFIPLDHKIRGIGLGGPAMILPIIGFFISSKEQSKILAI
                     MLIVSGILILIGGAAFLTISPSAEVENARNAMAEAGALFAVGAFQIALGMIKIRK"
     gene            432..959
                     /locus_tag="VLB45_05210"
     CDS             432..959
                     /locus_tag="VLB45_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007401585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HSD05134.1"
                     /translation="MSIETFVEWAISLVSDYLYGGIFVAALLETIFPPIPSEVIFPLA
                     GYIVSQNQMQFVHVIGLGLAGAAGSTVGALGIYFLALKVGRIALVKYLRYVRVTESHL
                     NRVDVWFTKHGEKVVFFGRMVPGIRELVSIPAGVLKMKITKFLIFTYAGSCVWCISLS
                     LAGYYFGVASVQLFG"
     gene            993..1694
                     /locus_tag="VLB45_05215"
     CDS             993..1694
                     /locus_tag="VLB45_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007401584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HSD05135.1"
                     /translation="MKAIILAGGMGKRLKPITDYVPKPLVPINNIPIIEWQIRYFKKF
                     GMKNFVICSGYKNSQIENYFNSRNLGVNIEHSVEKTPLGTGGAIKNASRFIDDENFFV
                     INGDVITDLNPKLLQSKPNSIAVIPLRTMFGVVDVVGDEIRQFQEKSHVTGQWMNAGV
                     YWLSKEIIKYLPKKGNIEDSTFSTFATKGHLFAIRYHDVFWYSIDSHKDIEECTKNIK
                     SRKYANFLDGKNSKK"
     gene            1691..>2157
                     /locus_tag="VLB45_05220"
     CDS             1691..>2157
                     /locus_tag="VLB45_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010194681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HSD05136.1"
                     /translation="MKLGYSLGSLLSVNEILECSKIMSNYEHDSIWIPETWGMEGYSM
                     LSAVSQIDRNARIGSSIINIYSRTPALIAMGAVTVDSISNGRLILGLGTSSEPIVEEW
                     HGVEFNVPVQRMREYVDIIRLVTSGKKVNYDGSLFQLKNFALLITPLRNKIPI"
BASE COUNT          723 a          323 c          442 g          669 t
ORIGIN      
        1 aaaaggatcc gatcatggat tcattccact tgatcataaa ataagaggaa ttggacttgg
       61 aggacctgca atgattttgc caatcattgg atttttcata tcaagtaaag aacagtcaaa
      121 aattttggca ataatgctaa ttgtgtcagg aattttaata ttgattggag gtgcagcttt
      181 tctaacaata tctccatcag ctgaagtcga aaatgcaaga aatgcgatgg cggaagctgg
      241 agcattattt gcagtaggcg catttcaaat agctcttggt atgataaaga ttagaaaata
      301 aatgcctatt tgtactaaat gtggtcaaga ggtaaaaaac actcattctt gtgatcatac
      361 aaatgatcaa gagttttgtg tagaatgtta taccgaatta cactactata tgacagaaac
      421 caaatgagaa tatgagcata gaaacatttg tagaatgggc aatttcttta gtatctgatt
      481 atctttatgg tggaatattt gtagcagcct tgttggaaac cattttccca ccaattccaa
      541 gtgaagtcat atttccattg gcaggataca ttgtttctca aaatcaaatg caatttgtgc
      601 acgttatagg actagggttg gcaggggctg caggatcaac tgttggtgcg cttggaattt
      661 attttttagc gctaaaagta ggaagaattg cgcttgtcaa atatttacga tatgtgcgag
      721 ttacagaatc tcatctaaat agagttgatg tttggtttac aaaacatggt gaaaaggtag
      781 ttttctttgg aaggatggtg cctggcatta gagaattggt ctcaattcca gcaggagtcc
      841 ttaagatgaa aatcaccaag tttctcattt ttacatatgc tggttcatgt gtatggtgca
      901 tatctttatc acttgcaggt tattactttg gcgttgcgtc tgtacaactt tttggatgaa
      961 cctttttatt gcaaaaaact tggattttat gcgtgaaagc aattatttta gcaggtggaa
     1021 tgggcaaaag gctcaaaccc attactgatt atgtaccaaa acctcttgtc ccaattaata
     1081 acattccaat catagaatgg caaatcagat attttaaaaa atttggaatg aaaaattttg
     1141 tgatatgttc agggtacaaa aattcacaga tagaaaatta ttttaattca agaaaccttg
     1201 gcgtaaacat agaacattct gtagaaaaga ctccattggg tactggtggt gcaataaaaa
     1261 acgctagtcg atttatagat gatgagaatt tctttgtaat taatggcgat gtgattactg
     1321 accttaatcc aaaattatta caatccaagc caaattccat tgcagtaatt ccgttgcgaa
     1381 ctatgtttgg tgtggtagat gtggtcggag acgagatcag acaatttcag gaaaaatctc
     1441 atgttacagg acagtggatg aatgctggag tatattggct ttctaaagaa atcataaaat
     1501 atttgccaaa aaaaggcaat attgaagatt caacattttc cacatttgct acaaaaggtc
     1561 atctttttgc aataagatac catgatgtgt tttggtattc tatagactca cacaaagaca
     1621 ttgaggagtg tacaaaaaat atcaaatcaa gaaaatatgc caatttctta gatgggaaaa
     1681 atagcaagaa atgaaacttg gatatagttt aggttcgttg ttatcagtaa atgaaattct
     1741 ggaatgctcc aaaataatgt caaattacga acatgattcg atttggattc ctgaaacgtg
     1801 ggggatggaa ggatattcga tgctgtctgc agtgtctcaa attgacagaa acgcaagaat
     1861 tggttcttct ataatcaaca tctattcaag aactccagct cttattgcaa tgggtgctgt
     1921 tacggtagat tcaatttcaa atggcagatt gatactggga ctaggcacta gtagcgaacc
     1981 catagtggaa gaatggcatg gggtcgaatt taatgtacca gttcagagaa tgcgtgaata
     2041 tgtggatata atcaggcttg taacatcagg aaagaaagtc aactatgatg gaagtttatt
     2101 tcaattaaag aacttcgcct tacttatcac acctttacgg aataagatcc cgatata
//
LOCUS       DATIDL010000118         2128 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrosopumilaceae archaeon isolate SMAG_U16342
            k141_1160726, whole genome shotgun sequence.
ACCESSION   DATIDL010000118 DATIDL010000000
VERSION     DATIDL010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935836
            Sequence Read Archive: SRR25158326
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosopumilaceae archaeon (soil metagenome)
  ORGANISM  Nitrosopumilaceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrosopumilales;
            Nitrosopumilaceae.
REFERENCE   1  (bases 1 to 2128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/04/2024 05:09:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,520
            CDSs (total)                 :: 1,474
            Genes (coding)               :: 1,474
            CDSs (with protein)          :: 1,474
            Genes (RNA)                  :: 46
            rRNAs                        :: 1 (5S)
            complete rRNAs               :: 1 (5S)
            tRNAs                        :: 43
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2128
                     /organism="Nitrosopumilaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1160726"
                     /isolate="SMAG_U16342"
                     /isolation_source="soil"
                     /db_xref="taxon:2202732"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            306..1049
                     /locus_tag="VLB45_05225"
     CDS             306..1049
                     /locus_tag="VLB45_05225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05137.1"
                     /translation="MSVEKEMTSGRNNISNYSTRPNLQNDLFDNIIGYEDIKKLFFLS
                     FESQRPIHILLVGPPASAKTLFMLGCMKLERSYFTLGTHSTRSGMVDYLFEKRPRFLI
                     VDEIEHMSIRDQTVLLSLMETGIIAETKFMKTRNTQLKTWVFATANGTNHLLTPLLSR
                     FMVLHFKQYKFENFLDVSIHMLGRDGIAKDIANQIAEQVWHKMKSKDIRDCIKIAHLA
                     RTKDDVNFIVETLLRYGKTFNSTDDKKQS"
     gene            complement(1180..1788)
                     /locus_tag="VLB45_05230"
     CDS             complement(1180..1788)
                     /locus_tag="VLB45_05230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05138.1"
                     /translation="MSSEVNIRESPNHFIVLDAIARGMNNVDKISGVSKLNKSEVEII
                     VNDLVFQRLVMMNEKRSFFGRKKNELKITETGMSLLGNKKKELQEKVQEMQQYYNNGD
                     KSQLDSFMVSNRSWVPMMLFAGIMDILFFTSMMSLLGLGLNPMESSLAQDGANADNSG
                     SADNTNGASDSSSESSGSDSQDVGSDGGGFDGGGFDGGGFDF"
BASE COUNT          713 a          386 c          353 g          676 t
ORIGIN      
        1 aaccataaat aataggtact aatagttagc agatatgtcg tttatcagat atcgtatgat
       61 gagattaaga aaatttacca aaaataaatt ttatgtgctc gtcaaatatc tgctatttgt
      121 ctacataagt ttcaagatga agattggctg gtactaatag gcaagagcaa tcgagtaatg
      181 tcataacgcg catttaaaat tcaacagtaa catccttaac atttcgtcta ctaaattaaa
      241 gtaattaggc acaatgattt tgcaggaaac tctataatca gtcaaatcaa ataattaatg
      301 cagaaatgtc tgttgaaaaa gaaatgactt ccggtagaaa taacattagc aattattcaa
      361 ctagacctaa tctgcaaaat gacctttttg ataacataat cggttacgaa gatataaaaa
      421 aactattctt cttgtccttt gagtcacaaa gaccaataca tattctactt gttggcccgc
      481 cggcgtcagc aaaaacatta ttcatgttag gttgtatgaa actcgaacgt tcttatttta
      541 cgctaggtac tcatagcaca agatctggaa tggtcgacta cttgtttgag aagcgaccca
      601 ggttcttgat tgttgatgag atagaacaca tgtctattag agatcagact gttctactta
      661 gtcttatgga aactggaata atagctgaaa caaaatttat gaaaactaga aatactcaat
      721 tgaagacatg ggtttttgca actgcaaatg ggaccaatca cttgcttact cctttgctgt
      781 ctaggtttat ggtactgcat tttaaacaat acaagtttga gaattttttg gatgtttcaa
      841 ttcatatgct cgggagagat ggcatcgcaa aggacattgc aaatcaaata gctgagcaag
      901 tttggcacaa gatgaaatca aaggacatta gggattgtat aaagatcgca caccttgcta
      961 gaaccaagga cgatgtaaat ttcattgtag aaactcttct aaggtatggt aagacattca
     1021 atagtactga cgataagaaa cagtcgtaga acttgatttt tgattactat tacatcttag
     1081 tagtccattt gttacacttt cgcagtaatc gagagtttta gcagaattgt actcgcttgc
     1141 ttttttttat gtatgtacat cattggattg aataaagatc taaaaatcaa aaccgccacc
     1201 atcaaaaccg ccaccatcaa aaccgccacc atcgcttcct acatcttgag aatctgaacc
     1261 ggaactttca gaactactat cactagcccc atttgtgtta tctgcacttc cactattgtc
     1321 tgcatttgcg ccatcttgag ctaaagaact ttccatagga tttaacccta gtcccaataa
     1381 agacatcatg gaagtgaaaa acagaatatc cattatccct gcaaacaaca tcatcggcac
     1441 ccatgatcta ttggaaacca taaacgagtc aagctgtgat ttgtctccat tattgtaata
     1501 ctgctgcatt tcttgaacct tctcctgtaa ttcctttttc ttgttgccaa gaagactcat
     1561 gccggtttct gttattttca attcattttt tttcctacca aagaaacttc tcttctcatt
     1621 catcattact aatctttgaa aaacaagatc attgactatt atttcaactt cagatttatt
     1681 taacttagaa acgccagaaa tcttgtcgac attattcatt cctcttgcaa tcgcatccag
     1741 aactatgaaa tgattaggac tttctcgaat attaacttca cttgacatgt caaatttatt
     1801 cacctgagtt tacttaaatg attcgttttt gacaaaattt agttagaatc tcaagtgcac
     1861 acagacttag aactttgtag attcccctct tgtattgagt ttttattcaa caaacttaaa
     1921 agtttaaaat tattaatcac aagaaattac acaattgggt ggagccgaca gtttcggagt
     1981 cgaaaatggg atgggcatta tagctgtaga atggatgaat tcatattcag tagagaaaaa
     2041 aaaggaattt aaagcaaaac ttgaaggtta ccggatctca tcaaaaaact ttggtgattt
     2101 tgaaataatc tcatggagcg gtgattgg
//
LOCUS       DATIDL010000119         4314 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrosopumilaceae archaeon isolate SMAG_U16342
            k141_1162898, whole genome shotgun sequence.
ACCESSION   DATIDL010000119 DATIDL010000000
VERSION     DATIDL010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935836
            Sequence Read Archive: SRR25158326
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosopumilaceae archaeon (soil metagenome)
  ORGANISM  Nitrosopumilaceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrosopumilales;
            Nitrosopumilaceae.
REFERENCE   1  (bases 1 to 4314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4314)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/04/2024 05:09:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,520
            CDSs (total)                 :: 1,474
            Genes (coding)               :: 1,474
            CDSs (with protein)          :: 1,474
            Genes (RNA)                  :: 46
            rRNAs                        :: 1 (5S)
            complete rRNAs               :: 1 (5S)
            tRNAs                        :: 43
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4314
                     /organism="Nitrosopumilaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1162898"
                     /isolate="SMAG_U16342"
                     /isolation_source="soil"
                     /db_xref="taxon:2202732"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..483)
                     /locus_tag="VLB45_05235"
     CDS             complement(<1..483)
                     /locus_tag="VLB45_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05139.1"
                     /translation="MEKIVNLHDKIQALESSQQEITLKLKRQRRMTGYAIVACSILVL
                     VMASYIPFLLEEAKGSPINTGRYVIENLIGDTTDTWLSWQLIPGKILSVNIVNPQIAD
                     EEKIQSIKNAILSEESIEIDDSLLHKGPKGTMSTYYVGWQGALKKVSEKDTKFYIPTT
                     F"
     gene            complement(1256..1423)
                     /locus_tag="VLB45_05240"
     CDS             complement(1256..1423)
                     /locus_tag="VLB45_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L40e"
                     /protein_id="HSD05140.1"
                     /translation="MPITDPLKKQIAQKARLHFKVCFACGAKNPMGATRCRKCRGAYL
                     RLKNRTLGIKK"
     gene            complement(1456..2373)
                     /gene="sucD"
                     /locus_tag="VLB45_05245"
     CDS             complement(1456..2373)
                     /gene="sucD"
                     /locus_tag="VLB45_05245"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HSD05141.1"
                     /translation="MVDLFEILIGKEGESDYKKKPVIVQGITGKFGSFHTQQMLAYGT
                     NIVAGVTPGKGGQKFENTPIYETVKEAVSATGAKISIIFVPAKFFRNAAVEALEAGIK
                     LLIAIPEHVPVRDAMTVLDLAKKKDAIMIGPNTPGIIIPGIIKIGIMPASPFKQGRIA
                     VLSRSGTLMYEVSYNLSAASFGQSISLGIGGDPINGTTVIEAFDMIRKMSNIDGVVVV
                     GEIGGDAEELLAQHIIDTKFDKPVVAYIAGRAAPKEKRMGHAGAIIYGTYGSAESKVS
                     MYAKANVPVAKRPAEVPMLLAGKLGGKKG"
     gene            complement(2366..3478)
                     /locus_tag="VLB45_05250"
     CDS             complement(2366..3478)
                     /locus_tag="VLB45_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HSD05142.1"
                     /translation="MRLLEYQAKSLFAEYGIRIPKGLTSTNIEQGRKDAKAIGFPFVI
                     KAQMAVGGRGKAGAIQKCHNADEFELKYPEIMQKVVKGEKTKAILLEQMQEYKKEIYL
                     SIFLNRGKRCYTIIASAEGGVEIESVKNQIIREVGLGNVDKNTAEEVAKAMGLKDNTV
                     GEFVNLVQKLSKMTIEKEAELAEINPVAVLNDGTLLALDGKVIIDDNAMFRHDELRKF
                     QEVSELEERAEKSGFSLVELDGNIAVVGNGAGLVMSSLDMLSDNGGKPACFLDVGGGA
                     TTETVYEALTLISKMKKVKGILVNLYGGIVKTTTVATAFLKAYEDKIIDIPVFARMSG
                     AEADKSKELLKGSRTKLFDTVEEAINAAVIGVNKIG"
     gene            complement(3517..3846)
                     /locus_tag="VLB45_05255"
     CDS             complement(3517..3846)
                     /locus_tag="VLB45_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05143.1"
                     /translation="MTKQATLDGWVLHHFTELLKKGSLNVAKIGTPIVLYRNTIEEDE
                     DSHEETVCTLTEGYVIVQVITSGGIIVPSIQQQFVFTLDQFPEWLMRKSRDLLLQCVD
                     YLEEQLK"
     gene            complement(3903..>4314)
                     /locus_tag="VLB45_05260"
     CDS             complement(3903..>4314)
                     /locus_tag="VLB45_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5915 domain-containing protein"
                     /protein_id="HSD05144.1"
                     /translation="LQKGSYVFDLGTTKITLDKEDFVIDFAEKTEYAMAKHESLIVFI
                     STARNRDMMARGLVRDVARRLQSLRKERGYNPTDILDAAYILDLDEESLEMLKEKHGE
                     LAFLVRVKKVEFSDKAKAYKDDDIDGQKIRISVE"
BASE COUNT         1141 a          942 c          710 g         1521 t
ORIGIN      
        1 aaatgttgta gggatgtaga acttggtgtc tttttcagaa actttcttta atgcaccttg
       61 ccatccaaca tagtaagtag acatggtgcc ttttggccct ttgtgtaaca atgaatcgtc
      121 aatttctatt gattcttcag acaagatggc attttttata gactggattt tttcttcatc
      181 cgcaatttga gggtttacaa tatttactga caatattttg ccgggaatca attgccacga
      241 taaccacgta tctgtagtat ctcctatgag attttcgata acatagcgac ctgtgtttat
      301 tggtgatcct ttcgcttctt ctaacaaaaa tggaatatac gatgccatga ctagtaccaa
      361 tatggaacat gcaacaattg catatccagt catcctacgt tgtcttttga gctttagtgt
      421 gatttcttgt tggcttgatt caagtgcttg aatcttgtca tgcaaattaa caattttttc
      481 cataaccata ctctcctcaa aattcttaat gtaagaaagc tcttcattca tcataatcca
      541 aatattctcc ttcttctgag tgacctaacc gataccatcg attcaaaatc aaaccatacc
      601 cagtattcgt gtctattctt cctcataatg ctatataaca ctatattatg tagtgttaga
      661 ctattattat tagtcgcaca caactagata gaatttttaa tgaaataatt tcataatttc
      721 ttttaaaaca catagggaat tagattttat tatatttgtt gcagtagaaa agatgcgcaa
      781 tattttatag aagttactaa aaaagtatga atacaattta tcagtaatct ctaaatctac
      841 atgtcacgta actaaaatag gcaccgaatg gatcccaaaa aataacttta aacggagatc
      901 gtcccttccg atccatttga ttatgtcatt ttttgtccat taagatcaat aataatgcga
      961 aagaacaaga cctgatggtt ttgttttcga tgattcaatt aataccgtat aggcttcaac
     1021 gaatcttatt ttacgctttt tcttcttcat agctaactcc ccactcgttt tttgtgtgac
     1081 cttgggtgtg atcaaatgtt tttttccacg agaattttgg cagaattaat tcctccatga
     1141 tttggagtat atgactagtc taatagataa aatagtaaga atgcactgca atgcaacgac
     1201 atgagaggaa tcagaaactt gctgatgctt cttgatgtga gatattttct atgaactact
     1261 ttttgatgcc tagcgttctg ttctttagtc taagataagc accacggcat tttcggcatc
     1321 ttgttgcgcc cattgggttt tttgcaccgc atgcaaaaca gaccttaaag tgcagcctgg
     1381 ccttttgtgc aatctgtttt ttcagcgggt cggtaattgg cattctatat ctctcttggc
     1441 tcactctatt atattctaac cctttttgcc accaagcttt cctgctaaaa gcattggcac
     1501 ttcggctggt ctttttgcta ccggaacgtt tgcttttgca tacatggaaa ctttggactc
     1561 tgcagagcca tacgttccat aaattattgc accagcatgc cccattctct tttcttttgg
     1621 cgcagctcgt cctgcaatat atgcaacaac tggtttatcg aattttgtat caatgatatg
     1681 ctgtgcaagc aattcctctg catccccacc aatttcccca actaccacaa caccgtcaat
     1741 gtttgacatt tttctaatca tgtcaaaggc ttcaattact gtggttccgt ttatcggatc
     1801 gccaccgatt ccaagcgata tgctttggcc aaagcttgca gctgaaagat tgtatgacac
     1861 ttcatacatc aaagtgccac ttcttgaaag aacagcgatt cttccttgtt taaacggcga
     1921 agcaggcatg attccaatct tgattattcc aggaattatg attccaggtg tgtttggtcc
     1981 aatcattatg gcgtcttttt tcttggcaag atcaagtact gtcatggcat ctcttactgg
     2041 cacatgttct ggtattgcta ttagcagctt gattccagct tctagtgcct caactgcggc
     2101 atttctgaaa aacttggcag gaacaaagat tatggaaatt tttgcacctg ttgcagaaac
     2161 cgcttctttt actgtctcat aaattggagt attttcaaat ttctgaccgc cttttcccgg
     2221 tgtcactcct gcgacaatgt tggtaccata tgctaacatt tgttgtgtgt gaaaagagcc
     2281 aaacttgcca gttatgcctt gcacaattac tggctttttc ttgtaatctg attctccttc
     2341 ttttccaatt aatatttcaa aaagatcaac caatcttgtt cactccaatt actgcagcat
     2401 taattgcctc ttcgaccgta tcaaacaact tggtacgaga accctttaac aattcttttg
     2461 atttgtcagc ttcagcacct gacattcttg caaagactgg tatgtcaatt attttatctt
     2521 catacgcttt gagaaaagct gtagccacag ttgttgtctt tactattccg ccatacaagt
     2581 ttaccaaaat tccttttact tttttcattt tgctgatcaa ggtgagtgcc tcatagactg
     2641 tttcagttgt cgcaccgcct ccaacatcca aaaaacaagc tggtttgcca ccattatctg
     2701 atagcatgtc aagtgacgac atgactagtc ctgccccatt tcctactact gcaatgttgc
     2761 catcaagctc aactaatgaa aatccgcttt tttctgcacg ctcttcaagc tccgaaacct
     2821 cctgaaactt tctaagctca tcatgtctaa acattgcatt atcatcaatt atcaccttgc
     2881 catcaagtgc caaaagtgtg ccgtcgttta atactgcaac tggattgatt tctgcaagct
     2941 ctgcttcttt ttctattgtc atctttgata atttttgtac tagatttaca aattcgccta
     3001 ctgtgttgtc ttttaggccc attgcttttg ccacttcctc ggcagtgttt ttgtccacgt
     3061 tacccagtcc tacctcacga atgatctggt ttttgacaga ctcaatttca acgcctccct
     3121 cagctgaggc aattatggtg taacaacgct tgcctctgtt caaaaatatg gaaaggtaaa
     3181 tttctttttt gtattcttgc atctgctcta gcaaaattgc cttggtcttt tcgcccttga
     3241 caactttttg cataatttca ggatacttta gctcaaattc gtcagcattg tgacactttt
     3301 gtattgcacc agctttgcct cttccgccta ctgccatctg cgcttttatc acaaaaggaa
     3361 aaccaatcgc cttggcatcc tttcttcctt gttcaatgtt agttgatgtt aatccttttg
     3421 gaattcgtat gccatattct gcaaacaaac tttttgcttg atactctaat aaccgcatgg
     3481 aaactttgga tttttaaggt ctttataatc tgttggttac tttagctgct cttcaagata
     3541 gtctacacat tgcaacaata gatccctgct ttttcgcatt agccactctg ggaattgatc
     3601 cagcgtgaaa acaaactgtt gttgaatgct tggaactatg attccgccag atgtgattac
     3661 ttgcacaatc acataccctt ctgtcaaagt gcagactgtt tcttcatgag agtcttcatc
     3721 ttcttctatc gtatttcgat atagtacaat tggcgttcca atctttgcaa cattaagtga
     3781 tccctttttt aataattctg taaagtgatg caatacccat ccgtcaaggg ttgcctgctt
     3841 tgtcatgtca ttaaaccatg atataattat ttaatcaagt cgaattgtga aaagcttacc
     3901 aatcactcta ctgaaatacg tattttttga ccgtcaatat catcatcttt gtatgctttg
     3961 gccttatctg aaaattccac tttttttacc ctcactagga aggcaagctc cccatgcttc
     4021 tctttgagca tctcaaggga ctcctcatca aggtctaaaa tataggctgc atcaagaatg
     4081 tctgtggggt tgtacccgcg ttcttttctt aatgactgga gtcttcttgc aacatctctt
     4141 accagaccgc gtgccatcat gtctcgattt cttgcagtgg agatgaaaac tatcaaggat
     4201 tcatgttttg ccattgcata ttctgttttt tctgcaaagt caatgacaaa gtcttcttta
     4261 tcaagtgtta ttttggtcgt gccaagatca aaaacataac ttcctttttg caat
//
LOCUS       DATIDL010000120         3424 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrosopumilaceae archaeon isolate SMAG_U16342
            k141_1567859, whole genome shotgun sequence.
ACCESSION   DATIDL010000120 DATIDL010000000
VERSION     DATIDL010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935836
            Sequence Read Archive: SRR25158326
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosopumilaceae archaeon (soil metagenome)
  ORGANISM  Nitrosopumilaceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrosopumilales;
            Nitrosopumilaceae.
REFERENCE   1  (bases 1 to 3424)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3424)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/04/2024 05:09:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,520
            CDSs (total)                 :: 1,474
            Genes (coding)               :: 1,474
            CDSs (with protein)          :: 1,474
            Genes (RNA)                  :: 46
            rRNAs                        :: 1 (5S)
            complete rRNAs               :: 1 (5S)
            tRNAs                        :: 43
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3424
                     /organism="Nitrosopumilaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1567859"
                     /isolate="SMAG_U16342"
                     /isolation_source="soil"
                     /db_xref="taxon:2202732"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            106..240
                     /locus_tag="VLB45_05265"
     CDS             106..240
                     /locus_tag="VLB45_05265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05145.1"
                     /translation="MIDTEYHYFDDEGNNVSKDLATKVEIITKDEDGLVIHAELVNIK
                     "
     gene            complement(245..697)
                     /locus_tag="VLB45_05270"
     CDS             complement(245..697)
                     /locus_tag="VLB45_05270"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HSD05146.1"
                     /translation="MKKEFHTGMVTRSSVVHASQQMAWKTLSNISNLPKWIIGIRKCV
                     ITSKKKKGIGSIRLVTFQDGSLLEEQIVTWNPGQSFSYMATGKTPLRAYFATLSIKPL
                     GKNSTKITWSGYIGTNKVTRHSFQKTFSEYDALYKNSISRLKSIIESR"
     gene            813..1787
                     /locus_tag="VLB45_05275"
     CDS             813..1787
                     /locus_tag="VLB45_05275"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HSD05147.1"
                     /translation="MKRKLGLVFLLLLVSLQIQTSTAISENKTLSPRDQAKAGIPIDK
                     ILCSEGLQPVMKKNNGNPACVKPTSVVKLIEIGWAIHVLPSYTKDEPKNSEIFGAGLY
                     QTIPQTEMYYENSTGFLARPSEYGTFPGVILIHEWWGVNDNIKEMAQHLASHGYVVLA
                     ADLYAGQVATTPDGARQLLTTFDTQKGITNLNSAAEHLVKQYGVDKLAILGWCFGGTQ
                     SLNYALSGNSLDATIIYYGQLVTDKDKLSMISWPVLGIFGGQDMNIPVETINNFESSL
                     NDLGIENEIYIYDNVGHGFANPSGANHSPDATKDAWQKTVSFLEKHLK"
     gene            complement(1842..2375)
                     /locus_tag="VLB45_05280"
     CDS             complement(1842..2375)
                     /locus_tag="VLB45_05280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05148.1"
                     /translation="MRQAFSIISKKYMPIIIGVAAAIIATTAAYAYVITNGQGDLANK
                     NNDFESIILDQDAQTYRINTQCEMIFAMTEGQYPDGESMPRIEITSLLEKYPDEFKEW
                     KAILEDPVKRQEFAQEVPREFNEVLIPIMMKEASINPELEPTAMLLIDPQASTKIKQA
                     FDENSCQEFFDSRQVNP"
     gene            complement(2403..2603)
                     /locus_tag="VLB45_05285"
     CDS             complement(2403..2603)
                     /locus_tag="VLB45_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008296784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05149.1"
                     /translation="MDSKITEQEKIALTPESGFNLVGIDPFEEPGKKLYLIEHFERYQ
                     EALEVKKSRKNPDEYLILYHGA"
     gene            2671..2868
                     /locus_tag="VLB45_05290"
     CDS             2671..2868
                     /locus_tag="VLB45_05290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05150.1"
                     /translation="MANWKKWGRIILWFGLIGAGVFIFLNLDVANRKRELYFLFGLSL
                     VLFISGWVGIFMSSKIGRALD"
     gene            complement(2865..>3424)
                     /locus_tag="VLB45_05295"
     CDS             complement(2865..>3424)
                     /locus_tag="VLB45_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HSD05151.1"
                     /translation="GVIVKEIPYAFTNRQSGSSKLDMSVGLDYAKAVWKLYRYGRSIR
                     EKEKRTSVHFLSKAGRFYTVGASGLFVNYLTAFVFGTLLSNLWYLHANMVGIIFSITS
                     NFILNKIWTFEDKDFGAKKTIKQYGLFLSFSGIGAVLQLLMVYYFIESHHMSYAIALL
                     LAVGIASIGNFVLNKKWTFKERIWS"
BASE COUNT         1046 a          632 c          601 g         1145 t
ORIGIN      
        1 gttatttctt gagtcaatcc aatcacttgg ttcagtatct gttttgtcag ccactttttg
       61 cttaattaaa acgtttttca atatcaagac agatcaatac tcgatatgat cgatacggaa
      121 tatcattact ttgatgatga aggaaacaat gtatcaaaag atcttgcaac caaagtggaa
      181 atcataacaa aagatgaaga cggtcttgtg atccatgccg aacttgtgaa cataaagtga
      241 tttcctatct gctttcaata attgatttta gccttgaaat cgagttctta tagagcgcat
      301 catactctga aaacgtcttt tggaatgagt gtctagtaac cttgtttgta ccaatgtatc
      361 cagaccatgt aatttttgtc gagtttttcc caagaggttt tattgacaag gtagcaaagt
      421 atgctctaag tggagtttta cctgttgcca tgtaagaaaa agattggccc ggattccaag
      481 ttacaatctg ttcttcaagc aaagacccgt cctgaaacgt aacgaggcgt atcgagccta
      541 ttcctttttt cttttttgac gtgatgacgc actttcttat tccaataatc cattttggca
      601 aatttgaaat attgcttaat gttttccagg ccatttgttg tgatgcatga actacagatg
      661 atcttgtcac catcccagta tgaaattcct ttttcatctc attttgttga caatatattg
      721 ataataaatc taattgtcat ttctgaataa atctaatttt taagtaatga tgatctatac
      781 tgtttaaatc tgatattgcc gtaattgaat ttatgaaaag gaaactaggc ctagtctttc
      841 ttttattatt ggtttcttta caaatacaaa ccagtacagc tatatccgaa aataaaaccc
      901 tgtcaccacg agatcaggca aaagcaggaa ttccaattga caagatattg tgttcagaag
      961 gactacagcc tgtcatgaaa aaaaataatg gcaatccagc atgtgtcaaa ccaaccagtg
     1021 ttgtaaaatt aatagaaata ggttgggcaa tacatgtgct accatcgtat acaaaagacg
     1081 aaccaaaaaa ttctgaaatc tttggggcag gtctatacca gaccataccc caaaccgaaa
     1141 tgtactatga aaattcaaca ggttttcttg cacgaccttc tgaatatggc acttttcctg
     1201 gagtgatttt gatacatgaa tggtggggag taaatgataa catcaaagag atggcccaac
     1261 atcttgcctc tcacggatat gttgtcttgg cagctgattt gtatgcagga caagtagcta
     1321 caactccaga tggtgcaaga cagcttctga caacatttga tactcaaaaa ggaattacta
     1381 atctgaattc tgcagcagag catcttgtaa aacaatatgg tgttgataaa ttggctattc
     1441 ttggatggtg ctttggtgga acccagtcac taaactatgc attgagtggc aatagccttg
     1501 atgcaacgat aatctattac ggacaacttg tcacggacaa agacaaactt tcaatgatta
     1561 gctggcctgt cttgggaata tttggaggcc aagacatgaa cattccagtt gaaactataa
     1621 acaattttga atcctcacta aacgatcttg gaatagaaaa tgaaatctac atctatgata
     1681 acgttggtca tggctttgca aatccatctg gagcaaatca ctcaccagac gctacaaaag
     1741 atgcttggca aaaaacagta tcatttttag agaaacattt gaagtaataa tgaattagaa
     1801 ttggaactaa gtacgttgat gattaaatct agaacgtaag gttatgggtt gacttgtctt
     1861 gaatcaaaga attcttgaca actgttttcg tcaaaagcct gttttatttt tgtgcttgct
     1921 tgaggatcga ttagtagcat tgcagttggc tctagttcag gatttatcga agcctctttc
     1981 atcatgatag gaatcaacac ttcgttgaat tctcttggaa cctcttgtgc aaattcttgt
     2041 ctttttacag gatcctccaa gattgccttc cattctttaa actcgtctgg atacttctct
     2101 aaaagacttg ttatctcgat tcttggcata ctttccccat ctggatactg gccttcagtc
     2161 attgcaaaga tcatctcaca ctgtgtgttt atcctatatg tctgtgcatc ttgatccaag
     2221 attatggatt caaaatcatt atttttgttt gcaagatcac cttgaccatt tgtaattaca
     2281 tatgcgtatg ctgctgttgt tgcaattatt gctgccgcca cacctattat tattggcatg
     2341 tatttttttg agattattga aaacgcctgt ctcaatccat tttgaaccag taaaaagtgt
     2401 tgttatgcac cgtggtatag tatcaaatat tcatccggat tttttcttga tttttttact
     2461 tctaatgcct cttgatatct ttcaaaatgc tctatcaggt acagtttttt tccaggttct
     2521 tcaaatggat ctattcctac tagattaaac ccagattctg gagtaagtgc gatcttttct
     2581 tgttctgtta ttttagaatc catttgcaaa ttttaccact ctgccatgat ataatcaatt
     2641 ttatatcata caaaacaact cgcttattac ttggcaaact ggaaaaaatg gggacgcata
     2701 atactgtggt ttggattaat tggagcggga gtatttattt ttctaaactt ggatgttgca
     2761 aatcgcaaaa gggaactata ttttctattt ggactgtcac tagttctttt catttcagga
     2821 tgggtgggaa tttttatgtc ttcgaaaata ggaagagcac tagactagct ccagatgcgt
     2881 tccttaaaag tccatttttt gtttaagaca aagtttccaa ttgaggcaat tcctactgcc
     2941 aaaagtaacg ctattgcata actcatgtga tgagattcta tgaaataata caccattaaa
     3001 agctgtaata ctgcaccaat tccgctaaag ctcaaaaaca aaccatattg ttttatggtt
     3061 ttttttgctc caaagtcttt gtcctcaaaa gtccatatct tgtttaagat aaagtttgat
     3121 gtaattgaaa aaataattcc taccatattt gcatgcaaat accaaagatt tgacaataag
     3181 gtaccaaata cgaaagcagt tagatagttg acaaacaatc cacttgcacc aacagtgtag
     3241 aatcgtccag cctttgatag aaaatgcaca gaggttcgct tttctttttc cctgatactt
     3301 cttccatacc tgtaaagctt ccatactgct ttggcatagt ccaatccaac agacatgtca
     3361 agtttacttg aacctgactg gcggtttgta aatgcatatg ggatttcttt tactatgact
     3421 cctt
//
LOCUS       DATIDL010000121         2973 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrosopumilaceae archaeon isolate SMAG_U16342
            k141_1164434, whole genome shotgun sequence.
ACCESSION   DATIDL010000121 DATIDL010000000
VERSION     DATIDL010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935836
            Sequence Read Archive: SRR25158326
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosopumilaceae archaeon (soil metagenome)
  ORGANISM  Nitrosopumilaceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrosopumilales;
            Nitrosopumilaceae.
REFERENCE   1  (bases 1 to 2973)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2973)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/04/2024 05:09:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,520
            CDSs (total)                 :: 1,474
            Genes (coding)               :: 1,474
            CDSs (with protein)          :: 1,474
            Genes (RNA)                  :: 46
            rRNAs                        :: 1 (5S)
            complete rRNAs               :: 1 (5S)
            tRNAs                        :: 43
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2973
                     /organism="Nitrosopumilaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1164434"
                     /isolate="SMAG_U16342"
                     /isolation_source="soil"
                     /db_xref="taxon:2202732"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..800)
                     /locus_tag="VLB45_05300"
     CDS             complement(<1..800)
                     /locus_tag="VLB45_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007550970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HSD05152.1"
                     /translation="MTGQKIDGVEVANSVKERVKKAASMLQTEGITPCLATVLVGENP
                     ASATYVKNKQKACTEVGIVTKDHKLPESFSQQEMNSLIDLLNNDETVHGILVQLPLPD
                     QLDEFSTISRISPIKDVDGLTPYSSGLLASGKAILKPCTPSGIMEMFDYYNIDLAGKH
                     AVIINRSNLVGKPLYHLLLEKNATVTTCHSKTKNLKEHCQRADIIITAIGDRSKFVLT
                     ADMIKEGAVVIDVGISRQNDKVVGDTDYTEIIKKAAYATPVPGGVGPMT"
     gene            978..1298
                     /locus_tag="VLB45_05305"
     CDS             978..1298
                     /locus_tag="VLB45_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HSD05153.1"
                     /translation="MKIEFDINEYIKNISKSNSYYHTFINRENLAAGILRLAPGQEDD
                     QTPHDSDEIYYVVRGDGFLSIAGRDYPVSEAKAYFVGKNIEHKFHGNKNELVVLYFFS
                     GPDS"
     gene            complement(1295..1912)
                     /gene="purN"
                     /locus_tag="VLB45_05310"
     CDS             complement(1295..1912)
                     /gene="purN"
                     /locus_tag="VLB45_05310"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007550967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HSD05154.1"
                     /translation="MINLAILISGRGSNMKAILKAIRKDNIPINPVIVISNKADAGGL
                     QIAKKLRVKTEVIESRGIKGGSWEYDKKIVSVLEKNNITPKNSLICLAGFMRIMSPEF
                     IRHFKGRIMNIHPAILPSFPGLHSQKQAIEYGVKYSGCTVHFVDEGIDTGPIIMQAVI
                     KIKKDDTEETLSKRILAKEHKIYCEAVRLFAEGKIKIIGRKTVIS"
     gene            2060..2347
                     /locus_tag="VLB45_05315"
     CDS             2060..2347
                     /locus_tag="VLB45_05315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05155.1"
                     /translation="MDVTDAQIKKAILSLAIEQGLLDMGRPVHEAVIGRLYKSYKCYL
                     PDCGDHPEYLNKILKDLYGNCYKTIIDSITKHLTEFSNRKQIEDFIKIISE"
     gene            2350..>2973
                     /locus_tag="VLB45_05320"
     CDS             2350..>2973
                     /locus_tag="VLB45_05320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05156.1"
                     /translation="MQTSLEKIQDNRFLVFSLLSIGIILLAVNFVSEDTRTLVANLIY
                     IPVPAALVILSIIIATRFRTLGAHGKAWIIFAVFAATWFIAEQIWMIYEIVYLIDPWP
                     SIADYFYIGGYPLLIMFSILYVYPLRKAISKKLVISALVISIAFLIPTFYTVYNANSE
                     ANEFEIALAASYPILDAIVLCPALIGVTLFFKGEVNFLWSLVCIGIIM"
BASE COUNT          864 a          533 c          520 g         1056 t
ORIGIN      
        1 gtcattggtc ctacgcctcc tggaactggt gtggcatatg cggctttttt gataatttca
       61 gtataatctg tatcgccaac aactttgtca ttttgtctgc ttatgccaac atcaattact
      121 actgcgcctt cttttatcat gtctgcagta agaacaaact tggatctgtc tcctatcgca
      181 gtaatgatga tgtctgctct ctggcagtgt tccttgaggt tttttgtctt tgaatggcat
      241 gtagtgacag ttgcattttt ttcaagaagt aaatggtata acggctttcc aactagattg
      301 cttcggttta tgattactgc atgttttcct gcaagatcta tgttatagta atcaaacatc
      361 tccatgattc cagatggtgt acatggtttt agtattgcct ttcctgaagc taagagccct
      421 gaactatatg gagtcaaccc atccacatcc tttattggag aaatccttga tattgttgaa
      481 aattcatcta gttggtctgg cagtggaagc tgaaccaaaa taccgtgaac cgtttcatca
      541 ttgtttagta aatctatgag cgaattcatc tcctgctgcg agaatgattc tggcagcttg
      601 tgatcctttg tgactattcc aacttccgtg catgcctttt gtttattttt tacataggta
      661 gctgatgctg gattctcgcc aactaacacc gttgcaagac atggagtaat gccttctgtt
      721 tgcaacatag aagcagcttt tttgactctt tctttaacag aatttgcaac ctctacccca
      781 tctatttttt gtccagtcac aagcttgagt actatgttgg atatttaatc tttggagtgt
      841 tttgatcatt gcactatggt atgttttgaa cagtactaga tatgtaaatt tgatcaaagg
      901 tgaattaaat aaaaatttgt ggtcgtgaca tacattcagg tagtcattaa taatgcatta
      961 tgcaaattaa taatacattg aaaattgagt ttgacataaa cgagtatata aaaaacatat
     1021 caaaaagcaa ttcatattat catactttta tcaataggga aaacttggct gctggaatac
     1081 tgagattagc tcctggtcaa gaagatgatc aaactccaca tgacagcgat gagatttatt
     1141 atgttgtaag aggagatggt tttcttagca ttgccggaag agactatcct gtatccgaag
     1201 caaaagcata ctttgttggc aaaaacatag aacacaaatt ccacggtaac aaaaatgaac
     1261 ttgtagtgtt gtattttttt agtggtcctg attcctaaga aattaccgtc tttctaccaa
     1321 ttatctttat ttttccttct gcaaaaagtc tgactgcctc acaataaatt ttgtgctctt
     1381 ttgcaagaat tctctttgaa agagtttctt ccgtgtcatc cttttttatt ttgattactg
     1441 cttgcatgat gattggtcca gtatctattc cttcgtctac aaagtgaact gtacagccag
     1501 agtatttgac gccatattca attgcctgct tctgtgaatg taagcctgga aaagagggta
     1561 aaattgcagg atgtatgttc atgattctac ccttgaaatg tctgataaac tctgggctca
     1621 ttatgcgcat aaaacccgca aggcatatca gactattttt tggtgttata ttgttttttt
     1681 ctaaaactga tacaattttt ttatcatatt cccaacttcc accctttatt cctcgacttt
     1741 caattacttc tgttttgact ctgagttttt ttgcaatttg aaggccacct gcatcggctt
     1801 tgttcgaaat tactattacc ggatttattg gaatgttgtc ttttctaatt gcttttaaaa
     1861 ttgctttcat attgctacct ctcccagaaa tcaagattgc aagattgatc aactgataaa
     1921 ctaatttttt cgtttaaata aagatatggc aattttttgt ctgtttatga ttgtacatat
     1981 taagttgtga atagtatttt acttttgtat tatctactac catcttgctc tttttaagca
     2041 ggctctatag attatcggca tggacgttac tgatgcccaa ataaaaaaag caatactctc
     2101 tcttgcaata gagcaaggtc tattggacat gggacggcca gtccatgaag ctgtaatagg
     2161 tcgcctctat aaaagttaca aatgctatct accagattgt ggtgatcacc cagagtatct
     2221 gaacaaaata cttaaagatc tatacgggaa ttgctataaa accataattg attcaattac
     2281 aaaacattta acagaattct ccaatcgaaa acaaatcgaa gattttataa aaataattag
     2341 tgagtaaaaa tgcaaactag tttagaaaag atacaagaca acagatttct ggttttttca
     2401 ctgctatcta taggcattat attactagct gtaaactttg tttccgaaga tactcgaaca
     2461 ctcgttgcaa atctgatcta cataccagta ccagctgcac ttgtaattct atcaataatt
     2521 attgcaacaa gatttcgtac acttggtgca catggaaaag cttggattat atttgcagtt
     2581 tttgctgcca cttggtttat tgctgaacag atatggatga tttatgaaat tgtgtacttg
     2641 atagatcctt ggccatctat agcagattat ttctacatag gtggctatcc tctcttgatt
     2701 atgttttcaa tactgtacgt gtacccgtta agaaaagcga tttcaaaaaa acttgtaatc
     2761 tccgcattag taatatcaat cgcattcttg attccgactt tttataccgt atataatgcc
     2821 aattcagaag caaatgaatt tgaaattgcg ttagcagcaa gctatcctat acttgatgca
     2881 attgttctat gtcctgcact gataggagtt acattgtttt tcaaggggga agtaaatttt
     2941 ctatggtcgc ttgtatgtat tggaattata atg
//
LOCUS       DATIDL010000122         3508 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrosopumilaceae archaeon isolate SMAG_U16342
            k141_2376774, whole genome shotgun sequence.
ACCESSION   DATIDL010000122 DATIDL010000000
VERSION     DATIDL010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935836
            Sequence Read Archive: SRR25158326
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosopumilaceae archaeon (soil metagenome)
  ORGANISM  Nitrosopumilaceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrosopumilales;
            Nitrosopumilaceae.
REFERENCE   1  (bases 1 to 3508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/04/2024 05:09:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,520
            CDSs (total)                 :: 1,474
            Genes (coding)               :: 1,474
            CDSs (with protein)          :: 1,474
            Genes (RNA)                  :: 46
            rRNAs                        :: 1 (5S)
            complete rRNAs               :: 1 (5S)
            tRNAs                        :: 43
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3508
                     /organism="Nitrosopumilaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2376774"
                     /isolate="SMAG_U16342"
                     /isolation_source="soil"
                     /db_xref="taxon:2202732"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..972
                     /locus_tag="VLB45_05325"
     CDS             <1..972
                     /locus_tag="VLB45_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007550724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HSD05157.1"
                     /translation="ILDTLKITSIAQRTEDSPDVKPYVQQTDTQWVTVELPPEMKEIQ
                     TLVETALGARYQELRRCGLSLSDNNSLSQLLRVREFVIRQNRRAAKPLFTAIRITHAL
                     NALESHGVTSFLRFCERTQEKKGVGIRDLFENDLNFSKAIRLAKAAQAKGIEHSKIIK
                     LKEILQAATGKSLVFTSYRDSVEIIYQKLVEMGISAGFLIGKAGEAGLKQKKQIETVQ
                     KFREGEYKVLVATRVGEEGLDISEVNLVVFFDNVPSSIRYVQRKGRTGRKDYGKLIVL
                     IAKDTTDEAYYWIGKRKVNAAKNMGEKMSKVLEKQSSSAKTGLDAYF"
     gene            complement(974..1258)
                     /locus_tag="VLB45_05330"
     CDS             complement(974..1258)
                     /locus_tag="VLB45_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05158.1"
                     /translation="MDENQTLEELVKRTIEGAIQSIPAHLYEVEASKDILKVVDQKEF
                     VYGLVMGMGLGMATAAVATLKQKMPTSEDMMRIKNLVYKMVPDIRENIFK"
     gene            1328..2104
                     /locus_tag="VLB45_05335"
     CDS             1328..2104
                     /locus_tag="VLB45_05335"
                     /inference="COORDINATES: protein motif:HMM:TIGR04296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEFG-CTERM sorting domain-containing protein"
                     /protein_id="HSD05159.1"
                     /translation="MSKLSKLLIITTVLILYVPVNSIAQIDSEISASTDKESYKPSER
                     VTITGTDNGNEVIPVTILVRNPIQNVYNVGQVNLQSGIFVHSFVLSDNSKPGIYTIDV
                     KHDSHHVKLQFIVTVGLVQNIPVDGSAIKVRGGELGLIKYKNAVVSTSDNSITIELDV
                     TSTSNEPIMQEFEIPKEVIDAPESLIIEVDGEILQCSETETNSARILNCFIPTDARTL
                     KITGTSVIPEFGPIAILVLIIGIIGIIFVSTKIRSRHGFY"
     gene            2126..2380
                     /locus_tag="VLB45_05340"
     CDS             2126..2380
                     /locus_tag="VLB45_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008299014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HSD05160.1"
                     /translation="MIVYILATCVPGNEKESISKLKALPEVLEVNGVMGKYDIFIKVA
                     ADDALKVDSAIAKVRSVPHITSTFTMAAIYGQGGTIDDEK"
     gene            complement(2381..3040)
                     /locus_tag="VLB45_05345"
     CDS             complement(2381..3040)
                     /locus_tag="VLB45_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007401455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05161.1"
                     /translation="MDVEKFTDDVYAIAQKLSEGKVAEVKSKINFVRERLIELYQQNL
                     VKINHSVLEIICAANLIEQNYTVDVEKHLSDILVCDVFASKGDGTFIIEIETGFTPPD
                     HALDTIDYYVARIASKIARYSKYCSKFALATPVVGILPIPKLFLKPPRERTEKEIKKI
                     KTLCDRFYKNPPIDLEDIVNARLHSIYLINIDKGFAKQVDPYGYLELVEKLQEKSELD
                     L"
     gene            3147..>3508
                     /locus_tag="VLB45_05350"
     CDS             3147..>3508
                     /locus_tag="VLB45_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010195448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HSD05162.1"
                     /translation="MSIKNITVLGSGIMGHGIAQVSAMSGYNVVLRDIEQQFLDKAME
                     KIKWSLDKLVSKQKISQQDAEKILSRITTKVDLAEALKDCDLMIEAVPEIMELKKKVY
                     SEVDKVADKKTVFASNTST"
BASE COUNT         1203 a          611 c          684 g         1010 t
ORIGIN      
        1 atacttgata ctctgaaaat caccagcatt gcacaaagga cagaagacag tccggatgtg
       61 aaaccatacg tacaacaaac cgatacgcag tgggttacag ttgagcttcc accggaaatg
      121 aaagagattc agacactggt agagactgca cttggtgcaa gatatcagga attacgaaga
      181 tgcggactca gtttatcaga taataattca ttatcacagc ttttgagagt cagagaattt
      241 gtgataagac aaaacagaag agccgcaaaa ccacttttta ctgcaattag aataactcat
      301 gcgctaaacg cattagagtc acatggtgtt acatcatttc ttagattttg tgaacgaaca
      361 caagaaaaga aaggagtagg aataagggat ctttttgaaa acgatctcaa cttttccaaa
      421 gcaatcagac tggcaaaagc agctcaagct aaaggcattg agcattcaaa aataatcaaa
      481 ctgaaagaga ttttgcaggc agcaactggc aaatcacttg tatttacaag ctatcgagat
      541 tccgtagaaa taatctatca aaagcttgtt gagatgggaa tctctgcagg gtttttgatt
      601 ggaaaagctg gcgaggctgg tctcaagcaa aaaaaacaga tagagacagt acaaaaattt
      661 cgggaaggcg aatacaaggt gcttgttgca accagagttg gagaggaagg actagacatt
      721 tctgaggtaa atcttgttgt gttttttgac aatgttccaa gctcgataag atatgttcag
      781 agaaaaggtc gtacaggacg aaaagattac ggaaaattaa ttgttctaat tgcaaaagac
      841 actactgatg aagcatatta ctggattggg aaacgaaaag taaatgccgc aaaaaacatg
      901 ggtgaaaaaa tgtcaaaggt tttagaaaag cagtcatcaa gtgcaaaaac aggacttgat
      961 gcatactttt agtttatttg aagatgtttt ctcttatgtc aggcaccatt ttgtatacca
     1021 aattctttat tctcatcata tcttctgacg ttggcatttt ttgttttaat gtagcaactg
     1081 ctgccgtagc cataccaaga cccataccca tcacaagacc ataaacaaac tctttttgat
     1141 ctacaacttt tagaatgtcc ttgcttgctt ctacttcata aaggtgggca ggtattgact
     1201 ggattgcgcc ttcgattgtt cttttgacta gttcttctaa tgtttggttt tcgtccatag
     1261 caaaataacc gtggtcaaca taataaatct acaattttta aatacactat gatttcaaca
     1321 gaaatggatg tcaaagttat caaagttgct aattatcacc acggttttga ttttatacgt
     1381 tccagtaaat tccatagccc aaatcgattc tgaaatatct gcatcaactg acaaagagtc
     1441 gtacaagcca agtgaaaggg taaccataac aggcacagat aatggcaatg aagtaattcc
     1501 tgtcaccata ctagttagaa atccaataca aaacgtctac aacgtaggac aagtgaattt
     1561 acaaagtggc atctttgtac acagttttgt tctcagcgac aactccaaac caggaatata
     1621 caccatagat gttaaacatg attcacatca cgttaaatta caattcatag taacagtagg
     1681 attagtacaa aatattccag tggacgggag tgccatcaaa gtgagaggtg gtgaattggg
     1741 tttgatcaaa tacaaaaacg ctgtagtttc aaccagtgac aactccataa ccatagaact
     1801 tgatgtcaca tcaacatcaa atgaacccat aatgcaagag tttgagattc caaaagaggt
     1861 catagatgcg ccagaatcat taatcataga agtagatgga gaaatactgc aatgctctga
     1921 gacagaaaca aattcagcta ggatattgaa ttgctttatt ccaacagatg ccagaacatt
     1981 aaagataact ggtacatctg tgattccaga gtttggccca attgctatac tagtactaat
     2041 cattggaata attggcatta tttttgtatc aacgaaaata agatcaagac atggttttta
     2101 ttagtagatc atttcagttt agctagtgat agtttacatt ctggcaactt gtgtacctgg
     2161 aaacgagaaa gagtcaattt caaaactcaa agcacttcca gaagtgttag aagtaaatgg
     2221 agtaatggga aaatatgaca tatttatcaa agtggcagca gacgatgcac taaaagtgga
     2281 ttctgcaatt gcaaaggtac gaagtgttcc gcacattaca agcacgttta caatggctgc
     2341 aatctatggc caaggcggaa ccattgatga tgaaaagtaa tcagagatct aattcacttt
     2401 tttcttgtaa tttttcaact agctcaagat atccgtaagg gtcgacttgt tttgcaaacc
     2461 ccttgtcaat gtttatcaag taaatagaat gcagtctggc attgacaatg tcttctaaat
     2521 ctattggagg atttttgtaa aatctgtcac atagtgtttt aattttttta atttcctttt
     2581 ctgttctttc ccgtggaggt tttaagaata gctttgggat aggcaaaatg ccaacaacag
     2641 gagtagcgag ggcaaatttt gagcagtact tactatatct agcaatcttg cttgcgattc
     2701 tggctacata gtaatcaatg gtgtccaagg cgtgatcagg cggagtaaat ccagtctcaa
     2761 tttctataat aaaggtgcca tcacctttgc ttgcaaaaac atcacacacg agaatgtcag
     2821 acaggtgttt ttctacatcc acagtataat tttgctctat cagattagca gcacagatta
     2881 tctcaagtac agagtggttt atttttacca gattttgttg gtatagttct atcaatcttt
     2941 cacgaacaaa atttatcttt gattttacct ctgccacctt tccttccgag agtttttgtg
     3001 ctatagcata aacatcgtct gtgaattttt ctacgtccaa aatcggcttt cctatggcat
     3061 ggtgttttaa gaagatagct gcttggtttt caaaaattca aagagattac tcataattta
     3121 aaaagggatt atagcaatca tccttgttga gcataaaaaa tatcacagtt ttaggctccg
     3181 gaataatggg acatggaatt gcacaagttt cagccatgtc agggtataat gtagtgctca
     3241 gagacattga gcaacaattt ttggacaagg ccatggaaaa aataaagtgg agtctggaca
     3301 agctagtatc aaaacaaaaa atttcacaac aagatgccga gaaaatttta tctagaataa
     3361 ccacaaaggt ggatcttgca gaagctctca aagattgcga cttgatgata gaggcagttc
     3421 cagagattat ggaattaaag aaaaaagttt attctgaggt agacaaggtt gcagataaaa
     3481 aaacagtctt tgcatcaaat acaagcac
//
LOCUS       DATIDL010000123        11449 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrosopumilaceae archaeon isolate SMAG_U16342
            k141_2174368, whole genome shotgun sequence.
ACCESSION   DATIDL010000123 DATIDL010000000
VERSION     DATIDL010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935836
            Sequence Read Archive: SRR25158326
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosopumilaceae archaeon (soil metagenome)
  ORGANISM  Nitrosopumilaceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrosopumilales;
            Nitrosopumilaceae.
REFERENCE   1  (bases 1 to 11449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/04/2024 05:09:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,520
            CDSs (total)                 :: 1,474
            Genes (coding)               :: 1,474
            CDSs (with protein)          :: 1,474
            Genes (RNA)                  :: 46
            rRNAs                        :: 1 (5S)
            complete rRNAs               :: 1 (5S)
            tRNAs                        :: 43
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11449
                     /organism="Nitrosopumilaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2174368"
                     /isolate="SMAG_U16342"
                     /isolation_source="soil"
                     /db_xref="taxon:2202732"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..262
                     /locus_tag="VLB45_05355"
     CDS             <1..262
                     /locus_tag="VLB45_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007550447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05163.1"
                     /translation="TSNVMITDMNLDDKWRQNRLAGQATKTPTEKPKEESKEKPKPQE
                     KVKAIKEKPKAEKESPKEKTLKSEEKKRKQTKAVKPAKKENK"
     gene            262..978
                     /locus_tag="VLB45_05360"
     CDS             262..978
                     /locus_tag="VLB45_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4e"
                     /protein_id="HSD05164.1"
                     /translation="MVSIAGSKKLKRQMAPVFWGITRKDSRFVVTVRPGGHSKHLSIP
                     SAVFLRDTLKLVTTLREAKSAIYGGKVKVDGVIRKSLHHGIGLMDVVELDGVSDIYRL
                     VPKNAFILQPIKINESEKTKKLVKLTSKTTIKGGQTQLGFHDGRSLIADTKANIGDTC
                     IMQIPEQKILDVIKLEKGCQVIVSSGINAGHIGKVNEIKDGTFVLPKRALVNIGEREI
                     EIPVDLVMVIGKDKPVVQVE"
     gene            983..1504
                     /locus_tag="VLB45_05365"
     CDS             983..1504
                     /locus_tag="VLB45_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HSD05165.1"
                     /translation="MSQKLAHPMKKITVAKVVLNMGVGKSGEPIEQARRALQQIAGQK
                     PNPRNAKATQRDWGVHKGEPIGVAVTLRRQPAIDLIKRLFAAKGNQISGSSFDNFGNL
                     SLGIKEHIDIPGVKYDPTVGILGLNISISLQRPGFNVRVRSKHKASVGGHHRITPEEA
                     KEFLTKEFGIQVV"
     gene            1506..1682
                     /locus_tag="VLB45_05370"
     CDS             1506..1682
                     /locus_tag="VLB45_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HSD05166.1"
                     /translation="MKNRDYKVTGRTEHKFGKGSRWCKRCGDYNAVIQKYDLDLCRRC
                     FREVAVYLGFRKFR"
     gene            1689..2081
                     /locus_tag="VLB45_05375"
     CDS             1689..2081
                     /locus_tag="VLB45_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008300278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HSD05167.1"
                     /translation="MPSNNILSNLFVTLFNTESRRKSECVVLPVSKLAMEVLKTLQNH
                     NYIGEFEHIDDKKGGKFKIQLLAKITKCGAITPRFKVKKGDYVNWEQTYLPSYSRGML
                     LVTTNHGVMSHHEAVKKGLGGMLIGYVY"
     gene            2071..2625
                     /locus_tag="VLB45_05380"
     CDS             2071..2625
                     /locus_tag="VLB45_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HSD05168.1"
                     /translation="MSTKLEFHEVSLPVPEKVKVSLVKRMLHVEGPLGKTRKDFKKIP
                     VELQADGKNVIIKSTGIRKKDYAIFNTVQSIIKSLMEGVQNGYTFKMKIVYAHFPITV
                     KVKDDNILVENFQGERAPRVSKIRGTTKIVVKGDDVILTGPVLADVSQTAASLQQNTK
                     VKNKDHRVFLDGLYLFEKHPGIEK"
     gene            2666..2739
                     /locus_tag="VLB45_05385"
     tRNA            2666..2739
                     /locus_tag="VLB45_05385"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2700..2702,aa:Asn,seq:gtt)
     gene            2834..3016
                     /locus_tag="VLB45_05390"
     CDS             2834..3016
                     /locus_tag="VLB45_05390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05169.1"
                     /translation="MQRKIPPPDKKILAHMATLSWNDLMLFMHKKYGKKFTQDFLRDY
                     KYRLQKLEWRKNQKWK"
     gene            complement(3235..3477)
                     /locus_tag="VLB45_05395"
     CDS             complement(3235..3477)
                     /locus_tag="VLB45_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05170.1"
                     /translation="MARTRRANRYCVDCGGRLVYYPRLSNPKAPNEHRVLVYACPDCT
                     KDFDKPKMFSIRRNVVEEPIETVEIEIVQAREKIPS"
     gene            complement(3674..4078)
                     /locus_tag="VLB45_05400"
     CDS             complement(3674..4078)
                     /locus_tag="VLB45_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HSD05171.1"
                     /translation="MESKVIALLESRNLLFLATIMKDGSPQISPVWADYENNHIMINT
                     AEGRIKHKNILRDPRVAVSIVDQNNPLNMTTIRGKVVEIISDYDYKHADKLTKKYMNK
                     DKYPFRREGEKRIIFKIKPERVFVLPELPTSA"
     gene            complement(4304..4555)
                     /locus_tag="VLB45_05405"
     CDS             complement(4304..4555)
                     /locus_tag="VLB45_05405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05172.1"
                     /translation="MRSDILVGGIIILFMGIGGYLWMKEYVVDCQNAPDTMPSFSSYL
                     GEICEQLKLVQSGVIFSAAAGFCIMIYGIAARKQIPKTS"
     gene            4727..5002
                     /locus_tag="VLB45_05410"
     CDS             4727..5002
                     /locus_tag="VLB45_05410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05173.1"
                     /translation="MVKQYEDTVASSSEVKKIVSDEVKPLLDKLTELVDKLERYHQHD
                     VEFQQRFLLLVSQMFRNQQISMDAFDQLDSFMRKEFFWGKTNSEKTE"
     gene            5297..5632
                     /locus_tag="VLB45_05415"
     CDS             5297..5632
                     /locus_tag="VLB45_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05174.1"
                     /translation="MFEILITTKQKLTDAQLKKLQEYFSEMPLEEILAGLNFARNRWT
                     AKDAGVLKVGRKSIIRREVHSITSEQAQWRLKNWKMMIANYRKRGYSYPTISRIKKIL
                     QEISNEDSK"
     gene            complement(5625..6263)
                     /locus_tag="VLB45_05420"
     CDS             complement(5625..6263)
                     /locus_tag="VLB45_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05175.1"
                     /translation="MTFISNWNQHKESLSQKVIDKVKPDAPLKPKIDEAQKKLQLQVV
                     KLDSITKKLKEKDEFIFKKVVESVRTHNKQYSQAYATELHEIRKMNKMVTSAKLAMEQ
                     IQIRLNTISELGDVVVTLSPCMSIIKGLGAGLGGLMPEANASMQDLSNILGDIMSGAS
                     VNGHENIAIETGSADTTTILEEAQSVLEGQIRSTIPDLPTDLPAPKRTEATI"
     gene            complement(6407..7528)
                     /locus_tag="VLB45_05425"
     CDS             complement(6407..7528)
                     /locus_tag="VLB45_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HSD05176.1"
                     /translation="MRTELKTALMMGIVLAIVIGSASTYFSSLEQSITSGNQENAQTQ
                     TSDKDIVSSIDKSRFKKAPDLVGISGYINTTPEDLKLKMKDSVILYDIWTYSCINCIR
                     TLPYITAWNEKYSDQGLLIIGIHSPEFEFEKDINNVKMAVQQYGITYPVVLDNDMKTW
                     KAFENRYWPRKYIADSEGYIRYDHIGEGDYDKTEKMIQQLLEERNKLLGLKITTEQPL
                     VNLQVHQFSPEQTPEIYFGYNFAIGRNQLGNSEGFKPENIVSYSLPTKFEKDHFYLEG
                     QWKNFEDRMRLESENGKIVLTYFAKSVNIVAANESNLQVILDGNPVTPESAGYDVKDG
                     ILQVSGDRLYNIISTKEASAHTLTIVAQPGFDIYTFTFG"
     gene            complement(7532..8266)
                     /locus_tag="VLB45_05430"
     CDS             complement(7532..8266)
                     /locus_tag="VLB45_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HSD05177.1"
                     /translation="MQEITIAVAAVAGLGSFLAPCILPMIPAFLSYISGTTITELQRN
                     NGTVNLASNRLSVFLNTLFFVLGFSIVFSIVGVIINSVLASSAGSLISGFNQVGGIII
                     IGFGSFMLLSSKINKLNFERKFLPNRAKASYSLSFLFGLAFATGWTPCIGPILGSILT
                     LAAATPGQAFTLLLTYSLGLGIPFILMGLFFSRFTRLIKSMSKHLKYYSVIMGSFIIL
                     MGILVYTNQLATIASFPLLNNLLLPG"
     gene            complement(8476..9606)
                     /locus_tag="VLB45_05435"
     CDS             complement(8476..9606)
                     /locus_tag="VLB45_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018039375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HSD05178.1"
                     /translation="MKHKFDFVNVDLEKIKSRNLYRKLQYSRIDGPYIIFKRKKLVNL
                     CSNDYLGLNVSQIAKRQIQSSSRLVSGNDFSFEILEEKLARHKSQESALTFPTGYMAN
                     LASIPTLAQKGDLILSDELNHASIIESCRLSGAKIVVYRHNDMVDLEKKSVLKANNKF
                     IVTEGIFSMDGDLANLREISSIATKNSAILVLDDAHGDFVVGSDGMGTGQYFGIAKKI
                     DLYVSSLSKGLGAFGGYVASRRNIIDLTINKAKPFIYTSALPNFLVDLAYKRFISNRE
                     GQRKKLWRNIKIMHKGLESLGYKTNSSSQIIPIIVGNEKKTMMFGKFLLDNGIFAQPI
                     RYPTVKLNQARIRISITAWLTKDHIEKTLDVLEIAGKKFKMI"
     gene            complement(9596..10576)
                     /gene="bioB"
                     /locus_tag="VLB45_05440"
     CDS             complement(9596..10576)
                     /gene="bioB"
                     /locus_tag="VLB45_05440"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007550476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HSD05179.1"
                     /translation="MNSEHAFITMCKEKILSGKNITSEEATTLINIPDESLSFLSDAA
                     NEITRKFNGNVIDVETLINAKKGTCPEDCSFCSQSAFYKTEIDTYKLLPVEIIIENAQ
                     KAKDEGVKSFCLVCAWRGPSEQDFEQICQIVEKVNECVGIEVNCSLGFINENMAKRLK
                     HLGVKRYNHNLEASRSFFSNICTTHTYQDRIDTAHVVKKNNLELCCGGILGMGETRMQ
                     RLELGLDLAELSPEECPINILVPQKGTPLELQAKLTLGEILRTIAVFRFLMPKTILKI
                     AGGREVHLANDQEKALLGGANGIITGGYLTIDGNSPKEDLQMISRIGLEA"
     gene            10931..11146
                     /locus_tag="VLB45_05445"
     CDS             10931..11146
                     /locus_tag="VLB45_05445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05180.1"
                     /translation="MASKRRSKKSKRRAKPAPTRKQLITVRAALNKAKRERDISRREG
                     RRLKNSLAVRNRMIRELGIKLRRARRR"
     gene            complement(11148..>11449)
                     /locus_tag="VLB45_05450"
     CDS             complement(11148..>11449)
                     /locus_tag="VLB45_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HSD05181.1"
                     /translation="KQFEARIDEIKNLDIVGDVRHKGMLMGIELVSNKRNRIPIKPEK
                     IIHQKIFSEAKKHNIYLRTLGHIVMLVPPLAISTNELEFLLDGTIDTIKKVTKVI"
BASE COUNT         3647 a         2021 c         2035 g         3746 t
ORIGIN      
        1 cacatcaaat gtaatgataa ctgacatgaa ccttgatgac aagtggagac aaaacagatt
       61 agcgggacag gcaacaaaaa cacctacaga aaaaccaaaa gaggaatcaa aagaaaagcc
      121 aaaaccacaa gaaaaagtaa aagcaataaa ggaaaaacca aaagccgaaa aagaatctcc
      181 aaaagaaaag actctaaaat ctgaggaaaa gaaaagaaaa caaacaaaag ccgtaaaacc
      241 tgcaaaaaag gagaataagt aatggtcagt atagcaggaa gtaagaaact gaaaaggcaa
      301 atggctccag tcttctgggg aataacaaga aaggattcac gatttgttgt tacagtaaga
      361 ccgggcggtc attcaaagca tctttcaatt ccaagtgctg tatttctaag agatacgcta
      421 aagctagtta ctacattaag agaagcaaaa tctgcaatct atggaggtaa agtcaaagtt
      481 gatggtgtta tacgcaaatc gttacatcat ggaataggcc taatggatgt ggttgagctt
      541 gatggagtct cagacattta cagacttgtt ccaaaaaatg cctttatctt acaaccaatt
      601 aaaataaatg aatcagaaaa aacaaaaaaa ctcgttaaac tcacaagtaa aactaccata
      661 aaaggcggac aaacacaact tggcttccat gatggacgat ctttaattgc tgatactaaa
      721 gcaaacatag gggatacctg cataatgcaa attccagaac aaaaaattct tgatgtaata
      781 aaactggaaa aaggttgtca ggtcatagta tcaagcggaa ttaatgcagg gcatattggt
      841 aaggtaaatg agatcaagga tggtactttc gtgcttccaa aacgagccct agttaatatt
      901 ggtgaaagag aaatcgaaat tccagtagat ctagttatgg tcataggaaa agacaagcct
      961 gtggtgcagg tagagtgata acatgtcgca aaaactagca cacccaatga agaaaatcac
     1021 ggttgccaaa gtagtgctaa acatgggagt tggaaaatca ggtgagccaa tagagcaagc
     1081 aagacgtgca ttacaacaaa tcgcaggaca aaaaccaaac ccaagaaatg ccaaagctac
     1141 ccaaagagat tggggtgtac acaagggaga accaataggt gttgcagtga cattgcgcag
     1201 acaacctgca attgatctta tcaaaagatt gtttgctgca aaaggtaatc aaatcagtgg
     1261 ttcttcgttt gacaattttg gcaatctttc gcttggaata aaagagcata ttgatattcc
     1321 gggagtcaag tatgacccaa cggttggaat tttaggatta aacatttcta tttctctaca
     1381 aagaccagga tttaatgtac gagtaagaag caaacacaag gcaagtgttg gaggtcatca
     1441 caggattacg ccagaagaag caaaggaatt tctaaccaaa gagtttggaa tacaggtagt
     1501 gtaacatgaa gaatcgagat tacaaagtca ctggaagaac agagcacaaa ttcggtaaag
     1561 gttctaggtg gtgtaagagg tgcggggact ataatgcagt aattcaaaaa tatgatttgg
     1621 atttgtgccg acgttgtttt agagaagtgg cagtatatct aggattcagg aagtttaggt
     1681 aataaaaaat gccatctaat aatatcctct caaacttgtt tgtaacacta ttcaatactg
     1741 aatctagaag aaagagcgaa tgtgttgtat tgccagtatc aaagcttgct atggaagtac
     1801 taaaaacact tcaaaaccat aattatattg gagaatttga gcatattgac gacaagaaag
     1861 gcggcaagtt caaaattcaa cttttagcta aaattacaaa atgtggggca atcacaccac
     1921 gattcaaagt caaaaaagga gactatgtaa actgggagca gacgtacctt ccatcatatt
     1981 ctagaggaat gttacttgta actacaaatc atggcgtaat gtcacaccat gaggcagtaa
     2041 agaaaggatt aggaggaatg ctgataggct atgtctacta aactagaatt tcacgaagtt
     2101 tcattaccgg tgcctgaaaa agtaaaggtt tcactagtaa aaagaatgct tcatgtagag
     2161 ggtcctttag ggaaaacaag aaaagatttc aaaaagattc cagttgaatt acaggcagat
     2221 ggaaaaaatg ttatcataaa atcgactgga atcagaaaaa aagattatgc gatattcaat
     2281 actgtgcaat ccataatcaa aagtttgatg gagggcgtcc aaaatggcta tacctttaag
     2341 atgaaaattg tatatgcgca ctttccaatc actgttaaag taaaggatga caatatttta
     2401 gttgaaaact tccaaggtga acgtgcacca agagtttcaa agatacgagg tacaaccaag
     2461 attgttgtaa aaggtgatga tgtcatactg acaggtccag tactagcaga tgtatcacaa
     2521 actgctgcca gcctccaaca aaacaccaaa gtcaaaaata aagatcaccg cgtttttctt
     2581 gacggattat atctttttga aaagcacccc ggtatcgaga aataagtaaa gaattaaatc
     2641 cgaataatct aggaacaagc ctattgccgc cctagctcag cgtggtagag cagctgactg
     2701 ttaatcagct ggtcgtcagt tcaagtctga cgggcggcgc ctaatatttc taaattcgaa
     2761 tttagaaaca tcttattttg atattggaca atcaaaccaa aggcttacag gtagtttgtt
     2821 tagatattat gaaatgcaac gtaagatccc gccaccagac aagaaaattt tagcacatat
     2881 ggctactcta agctggaatg atctcatgct attcatgcat aaaaaatatg gcaaaaaatt
     2941 cacacaggat ttcctaagag attacaagta tcgactacaa aaactagaat ggcgcaaaaa
     3001 tcaaaaatgg aaataatcat tttttatcta gaattttgac taattgaaaa ttttactggg
     3061 gtttagctct tgttcgtatc agaaaaagtt aggcaatctt ttggtgggca atgggggtgt
     3121 tttgaccctt ggctcgcata tgtacgcaca aatgttaaac cccccactaa aaatgcaatt
     3181 tttgaaaaaa tcaatgcatg cctaaactga cataaattaa agggggttta gcttttacga
     3241 aggaatcttt tccctggctt gtactatctc tatttctacg gtttctattg gttcttcaac
     3301 tacgtttcgt ctgatggaaa acatctttgg tttatcaaag tctttagtgc agtcgggaca
     3361 tgcatagacc aaaaccctgt gttcattcgg agcttttgga ttagaaaggc gtgggtaata
     3421 gactaatctt ccaccacaat cgacgcaata cctgttagcg cgtcgtgttc tagccaatac
     3481 ggacctcctg catgtatcct actgtagcga tctgtattag atctatcctg tctaatattg
     3541 tgaacacaaa tgtgatccca aataatcttt caaatttcag attatggaat atgaaaataa
     3601 tgctgtttta gtttgattgt ccaatatcaa aataagatgt ttctaaattc gaatttagaa
     3661 atactgtgta atgttatgcg cttgttggta attctggcaa gacaaaaacg cgttctggtt
     3721 ttattttgaa tattattcgt ttttcacctt ctcttctaaa tggatatttg tctttgttca
     3781 tgtatttttt ggttagtttg tcagcgtgtt tgtaatcgta atccgaaatt atctcaacaa
     3841 ctttgcctct aattgtagtc atattcaatg ggttattttg atcaactatt gatacagcta
     3901 cccgtgggtc tcgaagtata tttttgtgtt ttattcgtcc ttcggcagta tttatcatga
     3961 tgtgattatt ttcataatct gcccagactg gagaaatctg aggagagcca tctttcataa
     4021 tagtggccaa aaataaaaga tttctacttt caagtaacgc tattacttta gattccattt
     4081 tctttgtttc accatatctt tgttagtttc tgtgtgtttt ctaattacta gtactgtatt
     4141 atcacttctt tctatgatta tgtctgaaaa ccccgtctta gttttatggt tctaagagct
     4201 agatctagtg gttttacatt taactgaaaa atgtacatga tatctattaa cttgcaaatg
     4261 tttatgtttt atgtttctta aataggaata tcttaaatta tgtttagcta gtttttggta
     4321 tttgttttct tgccgcaatc ccataaatca ttatgcaaaa tccggctgct gcactaaata
     4381 ttactccaga ttgaaccagt ttaagttgtt cacatatctc gccaaggtaa gatgaaaaag
     4441 atggcatcgt atcaggggca ttctgacaat ctaccacata ttctttcatc cataaatatc
     4501 cgccaatgcc catgaacaaa ataattatgc ctcctacgag tatatctgat ctcatctgat
     4561 ttcttaagac cctagtagat atcaggctag atgtgtctca tgcacatact tatgatgaga
     4621 gatatagcaa atctcctatc atctcatatt gtggctaaaa ttctgttgcc ctattgataa
     4681 aaaatgattt cttgacttta aatatgcaaa tttcatacgt ataaacatgg taaaacaata
     4741 cgaagatacg gttgctagca gtagcgaagt aaagaagatc gtatctgatg aggtaaaacc
     4801 gctacttgac aaattgactg aactagtcga taaactagaa cgataccatc aacatgatgt
     4861 tgaatttcaa caacgcttct tattactagt gtcacaaatg tttagaaatc aacaaatttc
     4921 aatggatgcc tttgaccagc ttgattcttt tatgagaaaa gaattcttct ggggaaaaac
     4981 aaactcagag aaaacagaat aatatatcaa ctcacaaaat tcaattttct tactctgata
     5041 tgcagtatgt ggtactggaa tatgttatac gatttctaac tcataaaatc gctcatgttt
     5101 taaaaaaagc tgctgggtcg tctgggatgg cagttggtta cgaaaaagct gccgacccaa
     5161 cagttatcat tattaaaaat ctgttccata ttacatcacg tctgtgctag aatcgtacat
     5221 acatatccta ctaaaatcag atcaaaaatt tcataattca cattaaatag tgaataatac
     5281 caacacaaac gtgggcatgt ttgagattct cataaccacc aagcaaaaac tgactgatgc
     5341 tcagctaaaa aaacttcagg aatactttag tgagatgcct ttggaagaaa tacttgctgg
     5401 tcttaacttt gcaagaaacc gatggacagc aaaagatgca ggtgtgctaa aagttggacg
     5461 aaaaagcata attcggcgcg aggtccactc tataacttct gaacaggcac aatggagatt
     5521 gaaaaattgg aagatgatga ttgccaatta taggaaacgt ggatatagtt atcctaccat
     5581 ctctagaata aaaaagattc ttcaagaaat tagtaatgag gattctaaat agttgcttca
     5641 gtgcgttttg gcgcaggaag atctgttggt aaatctggaa ttgtacttcg tatttgtccc
     5701 tctagaactg attgcgcctc ttctaatatt gtggtagtat cagcacttcc tgtctcgata
     5761 gcaatatttt catggccatt tactgatgcg cctgacatta tgtcgcctag tatgtttgac
     5821 aaatcctgca ttgatgcatt tgcctctggc atgagacctc caagtccagc tcctaatcct
     5881 tttatgattg acatgcatgg actcaatgtt actactacat cacctaattc ggatatggtg
     5941 tttagtcgga tttggatttg ttccattgct aattttgcac ttgtaaccat tttattcatt
     6001 tttcttatct catgaagctc cgttgcatat gcctgtgaat attgtttatt gtgagttcgt
     6061 actgattcta caactttttt gaaaataaat tcatctttct cttttagttt ttttgtaatt
     6121 gaatcaagtt ttactacttg cagctgaagt tttttctgtg cttcatctat ttttggtttt
     6181 aatggtgcat ctggttttac cttgtcgatt actttttgtg aaagactttc tttatgctga
     6241 ttccaattac taataaaggt catgagtttg tatcctagtt gcaatcaaaa atctatgttt
     6301 taacctccca tgttccaatt cattatgtaa ctgcagttac attactctgc agttacattt
     6361 gtaatgtttc taaattcgaa tttagaaaca ttattcatag atgtgattag ccaaaagtga
     6421 atgtgtaaat atcaaaacct ggttgagcta cgattgttaa tgtatgagca cttgcttctt
     6481 ttgttgatat tatattatac aaccgatctc ctgatacttg tagtattcca tctttaacat
     6541 catatcctgc actttctggc gtaactgggt ttccatcaag tatgacctga agattggatt
     6601 catttgcagc aacaatgttg acagattttg caaaatatgt tagcacaatc tttccatttt
     6661 ctgattcaag tctcatcctg tcttcaaagt ttttccattg cccttccaga tagaaatgat
     6721 ctttttcaaa tttcgttggc aatgaataac tcactatgtt ttctggtttg aaaccttctg
     6781 aatttccaag ttgatttctt ccaattgcaa agttatatcc gaagtaaatc tcgggagtct
     6841 gttctggaga aaattgatgt acttgcaaat tcactaaagg ttgttctgtt gtaattttca
     6901 aaccaaggag tttattgcgt tcttctaaaa gctgttgaat catcttttcc gttttgtcat
     6961 aatctccttc cccaatatga tcatatctta tgtatccttc agaatcggca atgtattttc
     7021 taggccaata tctattttca aatgccttcc aagttttcat atcattatca agtacgactg
     7081 gatatgttat tccatattgc tgtactgcca tcttgacatt gttaatgtct ttttcaaatt
     7141 caaattctgg agagtgtatc ccaattatca ataacccttg atcagagtat ttttcgttcc
     7201 atgcggtaat gtaaggaagt gttctgatgc agtttatgca gctgtatgtc cagatatcgt
     7261 atagtattac gctgtctttc attttgagtt tgagatcttc tggtgtagtg ttgatgtaac
     7321 cagaaattcc tactaaatct ggtgcctttt tgaatctact tttatcaatt gatgagacaa
     7381 tatctttgtc agatgtctgt gtttgggcgt tttcttgatt gcctgatgta atggattgct
     7441 ctaatgaaga aaaatacgta ctagcacttc caataactat tgccagtacg attcccatca
     7501 tgagtgcggt ttttagttct gttctcattt tctaacctgg cagtaataga ttattcaaga
     7561 gtggaaaact tgctatggtt gctaactggt ttgtatatac tagaattccc atcaatatga
     7621 taaacgatcc catgattact gaatagtatt tcaaatgttt gctcattgat ttaatcaatc
     7681 ttgtaaatct ggaaaaaaat aatcccatca aaataaatgg aattcctaat cctagtgaat
     7741 atgtaagtaa aagtgtaaat gcttgccctg gtgttgctgc agctaaagta agtatgctgc
     7801 ccagaattgg gccaatacat ggtgtccatc ctgttgcaaa tgccaatcca aaaagaaatg
     7861 aaagtgaata gctagctttg gcacgatttg gaagaaactt tctttcaaaa tttaatttat
     7921 ttatcttgct tgaaagcaac ataaaagatc caaatccaat tattattatt ccgcctactt
     7981 gattaaatcc cgaaatcagg ctaccggcag aacttgctaa aacactattt atgattacgc
     8041 caacaataga aaacactatt gaaaaaccaa gaacaaaaaa tagcgtattt agaaaaacac
     8101 tgagtctgtt tgaggcaaga tttacggtac cgttatttct ttgtagttct gttattgtgg
     8161 ttccagaaat atatgataaa aaggcaggga tcataggcag tatgcatggt gctaggaatg
     8221 aacctaaacc tgctacagct gccacagcta tagtgatttc ttgcatcttg tcttgaaatt
     8281 gatattttga ctaaaatagg ttgttccaaa tctattccat atcattagca tatcaatttt
     8341 tattcttcat gtgtcagaca tgcattgtga ataagcgatt tatcatagtt ggaattgttc
     8401 ttgctgcggc aatagcggcg gcagtctttt ggaactacac tttcagaacg ggatagtttt
     8461 taattcattt ttgaattaaa tcatcttaaa ttttttgcca gcaatttcta aaacatcgag
     8521 tgttttttct atatgatctt ttgtaagcca tgcggtaata gaaattctaa tcctcgcctg
     8581 attgagtttg actgtaggat atcttattgg ttgtgcaaat atgccattat caagcaaaaa
     8641 ttttccaaac atcatagttt ttttttcatt tcctactatg attggaatta tttgactaga
     8701 agaattagtt ttgtatccaa gagattccaa acctttatgc ataattttga tatttctcca
     8761 gagttttttt ctttgtcctt ctctatttga aataaatctt ttgtaggcaa gatctacaag
     8821 aaaatttggt aacgctgaag tatagataaa tggtttggct ttgtttattg ttaggtctat
     8881 gatgtttctt cttgatgcaa catatcctcc aaatgctcca agtcccttac ttaggctgct
     8941 tacatacagg tctatttttt ttgcaattcc aaaatactgt ccagttccca tgccatctga
     9001 accaactaca aagtctccat gtgcatcatc taagaccagt atggcactat tttttgtagc
     9061 aattgatgaa atctctctta gattggcaag atcgccgtcc atgctaaaga tgccctctgt
     9121 aacaataaac ttgttatttg ctttcaatac tgactttttt tcaagatcta ccatgtcatt
     9181 atgtctgtat actacaattt ttgctcccga aagtctgcat gactcgataa tacttgcatg
     9241 attcagttca tcacttaaga tcaaatctcc tttttgtgca agagttggaa tagaagctag
     9301 gtttgccatg tatcctgtag gaaatgtcaa tgctgactct tgcgatttat gacgcgcaag
     9361 tttttcttct agtatttcaa acgaaaaatc atttcccgag actaatcttg agcttgattg
     9421 aatctgcctt tttgcaattt gtgatacatt taatccaaga taatcatttg agcaaagatt
     9481 gactagtttt tttcttttga atatgatgta tggaccatct attctactat attgtaattt
     9541 tctgtaaaga ttccttgatt ttattttttc aaggtctaca tttacaaaat caaatttatg
     9601 cttcaagacc aattcgtgag atcatttgta aatcttcttt tggcgaatta ccatcaatag
     9661 tcagatatcc tccggtaatg attccatttg cacctcctag aagagctttt tcttgatcgt
     9721 ttgcaagatg tacttctcta ccccctgcta tctttagtat ggtttttggc atcaagaatc
     9781 taaagacagc tattgttcta agtatttctc caagtgtaag ttttgcttgt aattcaagcg
     9841 gtgttccttt ttgcggtact agtatgttaa taggacattc ttcagggcta agttctgcaa
     9901 gatcaagacc aagctctaac ctttgcattc ttgtttcacc catgcctaga attccaccgc
     9961 aacaaagctc aagattgttt tttttcacaa cgtgcgcagt atcaatccta tcctgataag
    10021 tatgggttgt acaaatgttt gaaaagaaac ttcgtgatgc ttccaaattg tgattatatc
    10081 tttttactcc taagtgtttt agacgctttg ccatgttctc gtttatgaat cctaacgaac
    10141 aattcacttc aatacctacg cactcattga ctttttcaac aatttgacaa atttgctcaa
    10201 aatcctgttc tgatggacct cgccatgcgc aaaccagaca gaaacttttg accccctcat
    10261 ctttcgcttt ttgagcattt tctattatga tctctacagg taacaatttg taagtatcaa
    10321 tttctgtttt gtaaaacgca gattgcgaac aaaatgaaca atcctcaggg caagttcctt
    10381 tttttgcgtt aatcagagtc tcaacatcaa tcacattccc attaaatttt ctagtaattt
    10441 catttgctgc gtcagataaa aatgataatg attcatctgg tatgttaatt agtgtcgtag
    10501 cttcttctga agtaatattt tttccagaca aaattttttc cttacacata gtaatgaatg
    10561 cgtgttctga attcacaacg tctatctttt agaatctact aattaagatt atcataacgg
    10621 taaacctcaa aaatcataat ggtttacgct ttttctcagt ttgttttatt ttatcatggt
    10681 ggaacatata tgtcctctct caggtttgta ttcctgacca catttgttac atcttacaag
    10741 gcttgatttg cttgcaatac acgtgtgtat ctcacctgtt tgtaacactt tcttacaatg
    10801 tggacaaata cgaatctgtg gcatggtatt attacttcat tgtaatataa tattaggttt
    10861 gattcaaaga tgatcgggtt gaaaacgatt gatgcgccat gcttaattat tactatatgg
    10921 cagactgtgc atggcgtcta agagacgatc aaaaaaatca aaaagaagag ctaagccagc
    10981 gccaacacgt aagcaattga tcacagttcg tgccgcactc aataaagcta aaagagaacg
    11041 tgatatatca agacgcgaag gcagacggct aaagaattct ctagctgtac gaaatagaat
    11101 gattagagag ctcggaatta aacttagaag agcacgaagg cgataaatca gattaccttc
    11161 gtgacttttt ttattgtatc aatagtacca tcaagtaaaa actctaattc gtttgtagaa
    11221 attgcaagtg gaggaacaag catgacaata tgtcccaacg ttcgtaagta tatgttatgt
    11281 ttttttgcct ctgaaaaaat tttttgatga atgatttttt caggttttat tggaattcta
    11341 tttcttttgt tacttacaag ttcaattccc ataagcatac ctttatgtct cacatctcct
    11401 acaatgtcta gatttttgat ttcatcaatt cgtgcctcaa actgttttg
//
LOCUS       DATIDL010000124         2222 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrosopumilaceae archaeon isolate SMAG_U16342
            k141_1570637, whole genome shotgun sequence.
ACCESSION   DATIDL010000124 DATIDL010000000
VERSION     DATIDL010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935836
            Sequence Read Archive: SRR25158326
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosopumilaceae archaeon (soil metagenome)
  ORGANISM  Nitrosopumilaceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrosopumilales;
            Nitrosopumilaceae.
REFERENCE   1  (bases 1 to 2222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2222)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/04/2024 05:09:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,520
            CDSs (total)                 :: 1,474
            Genes (coding)               :: 1,474
            CDSs (with protein)          :: 1,474
            Genes (RNA)                  :: 46
            rRNAs                        :: 1 (5S)
            complete rRNAs               :: 1 (5S)
            tRNAs                        :: 43
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2222
                     /organism="Nitrosopumilaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1570637"
                     /isolate="SMAG_U16342"
                     /isolation_source="soil"
                     /db_xref="taxon:2202732"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(156..479)
                     /locus_tag="VLB45_05455"
     CDS             complement(156..479)
                     /locus_tag="VLB45_05455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HSD05182.1"
                     /translation="MTKFLKELKNGRFVCSECQKCNKLVWPPSDYCNKCFGMTVWRPI
                     STNAILIEFSKKDDTVFCIAEFEKTLRIIGTLKADYDKLVLGQEIKLVECSYDNVEKF
                     VFQAV"
     gene            complement(476..1585)
                     /locus_tag="VLB45_05460"
     CDS             complement(476..1585)
                     /locus_tag="VLB45_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016940355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HSD05183.1"
                     /translation="MNKVALAGFGLTKFTRDDIAIENLALSAVKSLFDNTKNLSQKDV
                     EVVLTSTNSNAKYLSSIVSEISGIEPKISHSVESLCNSGANSVISAFSYIASGMADIA
                     LVVGAERTDSPGLVLDWDQSRGEYKHPIYWASMFTKSHMRRYGTTKEDLAIVSAKNHK
                     NALSNPFAYFEKEYSVDEIMNSKNLTDDLRLLDCSLPCNGSAAIILASEKMAKKITDT
                     PIWITGIGQKTISASFTKNIDLTSMKSTIDAATLAYKMSKRTPKDIDVAEIHDAFTIC
                     EIIELEDMGFAKKGEGKDLVRNIYETGDKMINPRGGLIGTGHPLGATGIAQIVEIGQQ
                     LQGKSEKKQLSGAKIGLVQNMSAAATSSSVLILES"
     gene            complement(1582..>2222)
                     /locus_tag="VLB45_05465"
     CDS             complement(1582..>2222)
                     /locus_tag="VLB45_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HSD05184.1"
                     /translation="QRMREYVDIIRLVTSGKKVNYDGSLFQLKNFALLITPLRNKIPI
                     YLAAVNQKMVELTWEIADGVIFYLRPITELKTTIQAMQSKKKIEIASQFITCVSNNSE
                     KARDRAKKTLAFYISVGKIYREFLSKNGFRNETQNIYDEFKKSGFTSHHELVTENMLD
                     SLTICGTPDESRKKLEQFVSIGVTLPIIQFNPIDDVIDSFKLLVSALSGDEI"
BASE COUNT          625 a          409 c          361 g          827 t
ORIGIN      
        1 tgggtaattt tgtagaacct gttgttttaa ttaatgtcgt atgttttaga tgttctttta
       61 ttctgtacat ggctatcttc gaattctcaa cgtatagaat tcatggtaat tatgaaatta
      121 ccagtgtaaa tcggtttgaa ctatatccaa atttgtcata ctgcttgaaa aacaaatttt
      181 tccacattat cataactaca ctctacgagt ttaatttctt gtccgagtac tagtttgtca
      241 taatctgctt ttagtgttcc aattattctc aatgttttct caaactctgc gatacaaaac
      301 acagtatcgt ctttttttga gaattctatc agaatggcgt ttgtagaaat aggtctccac
      361 acagtcatac caaaacattt gttacaataa tcactggggg gccagacaag tttattgcat
      421 ttttggcact cactgcaaac aaatcttcca ttttttagct cttttaggaa ttttgtcatg
      481 actccaggat taaaacagat gatgaagttg ctgccgcaga catgttttgg acaagaccaa
      541 ttttggctcc agataactgc tttttttcag atttaccttg aagttgttga ccaatttcaa
      601 caatttgtgc tattccagtg gctccaagtg gatgtcctgt tcctatcaga ccgcccctag
      661 ggtttatcat tttatcgcca gtctcgtaga tatttctgac aagatctttt ccttctcctt
      721 ttttagcaaa acccatatct tcaagttcga ttatttcaca aatagtaaat gcgtcgtgta
      781 tctcagctac atcaatgtct ttcggagttc gttttgacat cttatatgct aatgttgctg
      841 catcgatagt agatttcata cttgtaaggt caatattttt tgtaaagctt gcagaaattg
      901 ttttttgtcc tattccagta atccatattg gagtatcagt gatttttttt gccatttttt
      961 cagatgcaag aattattgca gcagaaccat tacacggcaa cgaacaatcc aatagcctta
     1021 gatcatctgt aagatttttc gagttcatta tttcatcaac agagtattct ttttcaaaat
     1081 atgcaaaagg atttgataat gcattcttat gattttttgc cgaaacaatt gcaagatctt
     1141 cttttgtggt gccatacctt cgcatgtgag atttagtaaa cattgatgcc cagtatattg
     1201 gatgtttgta ttctcctcgt gattgatccc aatctaagac taacccagga ctgtcagttc
     1261 tttctgcccc aactactagt gcgatatctg ccattcctga tgcaatataa gagaacgcag
     1321 agattacact atttgctcca gagttacata gactttctac agaatgtgaa atttttggtt
     1381 caattccaga gatttcagat acaatacttg atagatattt ggcgttgcta ttagttgatg
     1441 taagtactac ctctacatct ttttgagata gatttttggt attatcaaat aatgatttaa
     1501 ccgcagaaag tgcaaggttt tcaatagcga tatcatctct agtaaatttc gtaaggccaa
     1561 atcctgctaa tgctacttta ttcaaatttc atcccctgaa agcgcagata cgagtagctt
     1621 gaatgaatct attacatcgt caattggatt aaactgtatt attggtaatg tcactcctat
     1681 actaacaaac tgttctagtt tttttctaga ctcatcaggt gttccacata tagtaagtga
     1741 atcaagcata ttttctgtaa ccagttcatg gtgtgacgta aatcctgatt ttttaaattc
     1801 atcgtatatg ttttgagttt cgtttcgaaa tccatttttt gataaaaact cacggtaaat
     1861 ttttcctacg gatatataaa atgctaatgt cttttttgct ctatctctag ctttttcgga
     1921 gttatttgaa acacatgtga tgaattggct cgctatctcg atttttttct ttgattgcat
     1981 tgcttgtatg gtggttttta attcagtaat gggcctaaga taaaaaataa ccccatctgc
     2041 aatttcccat gtaagctcca ccattttctg atttactgca gccaaatata tcgggatctt
     2101 attccgtaaa ggtgtgataa gtaaggcgaa gttctttaat tgaaataaac ttccatcata
     2161 gttgactttc tttcctgatg ttacaagcct gattatatcc acatattcac gcattctctg
     2221 aa
//
LOCUS       DATIDL010000125         3412 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrosopumilaceae archaeon isolate SMAG_U16342
            k141_564412, whole genome shotgun sequence.
ACCESSION   DATIDL010000125 DATIDL010000000
VERSION     DATIDL010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935836
            Sequence Read Archive: SRR25158326
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosopumilaceae archaeon (soil metagenome)
  ORGANISM  Nitrosopumilaceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrosopumilales;
            Nitrosopumilaceae.
REFERENCE   1  (bases 1 to 3412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3412)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/04/2024 05:09:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,520
            CDSs (total)                 :: 1,474
            Genes (coding)               :: 1,474
            CDSs (with protein)          :: 1,474
            Genes (RNA)                  :: 46
            rRNAs                        :: 1 (5S)
            complete rRNAs               :: 1 (5S)
            tRNAs                        :: 43
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3412
                     /organism="Nitrosopumilaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_564412"
                     /isolate="SMAG_U16342"
                     /isolation_source="soil"
                     /db_xref="taxon:2202732"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..280)
                     /locus_tag="VLB45_05470"
     CDS             complement(<1..280)
                     /locus_tag="VLB45_05470"
                     /inference="COORDINATES: protein motif:HMM:NF012870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HSD05185.1"
                     /translation="MKKPNQIVQMDILGPFYIQNCAQKNYFISCIDDCSRKVSSERSE
                     RKRSVDVLQILEDYIVENGKPEKVMHDNGKQFTSKTFRRFLQRNKIKDK"
     gene            919..1533
                     /locus_tag="VLB45_05475"
     CDS             919..1533
                     /locus_tag="VLB45_05475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05186.1"
                     /translation="MNIFSVQGKIISQTITSFDGLKIQVMDDDQQWFEDRNDDLLGSS
                     WVNNDGSFEISFDESSFKDTWVEMEPRIYLIIRNKDGQIIHRTTTYKIQIDDKRETTI
                     KIPITVSIDSLKKKPELTADIDPYANNNNRILSAFSSLNDVYTINNSDFVRNFTLLNN
                     SINAWLVYAQEFSWKRIGYDGPQVPRYPRKQPDHKHTLSWEKMA"
     gene            1530..>3412
                     /locus_tag="VLB45_05480"
     CDS             1530..>3412
                     /locus_tag="VLB45_05480"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HSD05187.1"
                     /translation="MKKNCIYGTEQFLKRTENYDVINSDIVCNADVCIIGSGAAGAIL
                     GTKIAKAGNSVVILEKGGYYDGESMNQRESDMIPLLWKNAGANFTSNLRIAIAQGSCL
                     GGSTVINDAVCFRIPKLVIKQWNVMGVNISEAEWDKANDEVAKRINMVEVTDQELNTN
                     AKKLKEACENFKIEGNSIEHSKNFRNCGQSITDPSLKSCVKCGFCHMGCHYDTKQSML
                     VTYIHDAMIDDTLDYTVYCNCSADMITYENGVATGVNGKFTDSSNNETYRIRVNAKVI
                     IVSAGSIASSNLLQKSLIGGEKVGKGLALHPAPFVMGLFDEEIRGNRGIPMSYTCHEF
                     GITNGVQEGGFLIESIFLPIFQMALAIPSFGLDHAKMMKNYNHYTMAGIMTRDDPVGK
                     VLMSDNGNPKVVYNLSEQTANDMARGMGILARMWFNIGAKTVITSHRDVYEIGTRADV
                     PKLKDVVRNNPDGLMLGSAHPQGGNKMGDDENNCVVDSNCKVFGFNNLYVCDASVFPT
                     ALGVNPQLTVMALASITADKIIKKLQEISFISKDIGKTCDVTQPRFCTTEQLSDRFAV
                     TDHKEDLFQLLANSNEGTKIVPGNNWNFDRQTLKIYNNLYWKGFYGRDANLMTTVLRY
                     FGG"
BASE COUNT         1139 a          507 c          633 g         1133 t
ORIGIN      
        1 gtttatcttt tattttgttg cgttgtaaaa aacgtctgaa tgttttagat gtgaattgtt
       61 taccattgtc atgcattacc ttttcaggtt taccattctc tacaatgtag tcttcaagaa
      121 tctgcaaaac gtctacactt ctttttcttt cagacctttc acttgatacc tttcttgaac
      181 aatcatcaat acaactgata aaatagttct tttgagcaca gttttgaatg taaaaaggac
      241 ctaaaatatc catctgtact atctggtttg gttttttcat tgcaaatctt ttgtattctc
      301 tatttctgat tttacatgaa aggatgttct atccatgtct tttcagtatt tggtatatgg
      361 ttcctgtact gaatgatatc tgctttagcc tttttaacct gaatcttata ccattacatc
      421 caaatcgttt gattgtacgt aaattgatta tttcttgttc tatctctttt gttacttttc
      481 caggttgtat attatgtggt tttctacttc tatccttaag accttctatt ccatacttta
      541 ggtacctgtt cttccacttg tagtatgttt ttgtagatgt attatatcta gtacagatat
      601 cagttactgt atctcctgtc cattcttttt catgaattaa attatatttt gtttgtaatt
      661 ctatttttca tacacttcct agcagaacta gaaagtgtta cctatgtcag taaatgaaat
      721 tgtaacctat gttagcaagt acaacaacta gatatacaat aaattgcttg gcaacaaaat
      781 ttgaaatttg tatcagcctt tgtatgtttt tttcaaagca atttttgtat atcataagtt
      841 aataacaatt tttaatgatc ttttgtacgc cattgattat gtaaaagatc ttatatactt
      901 gtattttata actattttgt gaatattttt tcagttcaag gaaaaataat cagtcaaaca
      961 atcacttctt ttgatggtct aaaaattcag gtaatggacg atgatcaaca gtggttcgaa
     1021 gatcgcaatg atgacctatt gggatcgagc tgggtaaaca atgacggttc ttttgaaatc
     1081 agttttgacg agtcgtcctt taaggatacc tgggtggaaa tggaaccaag aatatatctg
     1141 ataataagaa ataaagatgg acagataata cacagaacta ccacgtataa aattcaaata
     1201 gatgataaaa gagagacaac tattaaaata cctataacag tttctattga ttcgctaaaa
     1261 aagaaaccag aattaacagc agatatagat ccgtatgcaa acaataataa tagaattttg
     1321 tcagcgtttt cttctttaaa tgatgtttat acaataaaca acagtgattt tgttagaaat
     1381 tttacacttt tgaataattc gattaatgca tggttagtat atgcccaaga attttcttgg
     1441 aaaaggattg gatatgatgg tccacaggtt ccaagatatc cgcgaaaaca gccagatcat
     1501 aaacatactt tatcatggga gaaaatggca tgaaaaagaa ttgtatatat ggaacagaac
     1561 aatttttaaa acgcacagaa aattatgatg taattaattc agacatagtc tgcaatgcag
     1621 atgtctgtat aattggttct ggtgctgctg gagccatatt gggtacgaaa attgccaaag
     1681 ctggaaattc tgttgttatc ttggaaaaag gaggatatta tgatggggaa tccatgaacc
     1741 aaagagagtc ggatatgatt cctcttttat ggaaaaatgc aggagcaaat tttacctcta
     1801 atttgagaat agctattgca caaggatctt gcctaggtgg ttcaacggta ataaatgatg
     1861 ctgtctgttt tcggattcct aaattagtta ttaaacagtg gaatgtaatg ggcgttaata
     1921 taagtgaagc tgaatgggac aaggccaacg atgaagttgc aaaaaggatc aacatggtag
     1981 aggtaactga ccaagaatta aacacaaatg ctaaaaaact gaaagaagca tgcgaaaatt
     2041 tcaaaattga aggaaactct attgaacatt caaaaaattt tagaaattgc ggacaatcaa
     2101 taacagatcc atctctaaaa tcctgtgtta aatgcggatt ctgtcatatg ggttgccatt
     2161 atgacactaa acaaagtatg ttagtaacat acattcatga cgcaatgata gatgatactt
     2221 tagattatac ggtatactgt aattgtagtg cggatatgat cacatatgag aatggtgttg
     2281 caacgggtgt aaatggaaaa tttacagatt ccagtaacaa tgagacttat cggatacgtg
     2341 ttaatgccaa agtaattatc gtgtctgctg gatctattgc atcgtcaaat ttactacaga
     2401 aatctctaat tggtggtgaa aaagtaggaa agggtttagc attacatcca gcaccttttg
     2461 ttatgggtct ttttgatgaa gaaattcgag gaaatagagg gattccgatg tcatatacat
     2521 gtcatgaatt tggcataaca aatggagtac aagaaggagg attcttgata gaaagtatat
     2581 ttttgccaat ttttcaaatg gcacttgcaa tacctagttt cgggttggat cacgccaaaa
     2641 tgatgaaaaa ttacaatcat tatacaatgg ctggaataat gactagagat gatccagttg
     2701 gaaaagtatt gatgtcggac aatggtaatc caaaagtcgt ttacaatcta tcagaacaga
     2761 ctgcaaatga tatggctaga ggaatgggaa ttttagctag aatgtggttc aacattggtg
     2821 caaaaacagt aattacatcg catagggatg tctatgaaat aggaactaga gcagatgttc
     2881 caaaactaaa ggatgttgta cgtaataatc cagatggcct catgcttggc tctgcacatc
     2941 cacaaggtgg aaacaaaatg ggagatgatg aaaataattg cgttgtcgat tccaactgca
     3001 aggtatttgg gtttaataat ctctacgtat gtgatgcaag tgtttttcca acagcattgg
     3061 gggtcaatcc tcaactaact gtaatggctt tagcttctat aacagcagac aagattatta
     3121 aaaagttgca agaaatttcg ttcatttcta aagatatagg aaagacatgt gatgttacac
     3181 agccaagatt ttgtactacc gaacaactct ctgacagatt tgctgttact gaccataagg
     3241 aagatttatt tcaattactg gctaattcaa acgaaggtac aaagatagtt cctggcaata
     3301 attggaattt tgatcgtcaa acactcaaga tttataataa tttgtattgg aagggtttct
     3361 acggcagaga tgctaattta atgactactg tcttgagata ttttggtggc tt
//
LOCUS       DATIDL010000126         9770 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrosopumilaceae archaeon isolate SMAG_U16342
            k141_1169593, whole genome shotgun sequence.
ACCESSION   DATIDL010000126 DATIDL010000000
VERSION     DATIDL010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935836
            Sequence Read Archive: SRR25158326
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosopumilaceae archaeon (soil metagenome)
  ORGANISM  Nitrosopumilaceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrosopumilales;
            Nitrosopumilaceae.
REFERENCE   1  (bases 1 to 9770)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9770)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/04/2024 05:09:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,520
            CDSs (total)                 :: 1,474
            Genes (coding)               :: 1,474
            CDSs (with protein)          :: 1,474
            Genes (RNA)                  :: 46
            rRNAs                        :: 1 (5S)
            complete rRNAs               :: 1 (5S)
            tRNAs                        :: 43
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9770
                     /organism="Nitrosopumilaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1169593"
                     /isolate="SMAG_U16342"
                     /isolation_source="soil"
                     /db_xref="taxon:2202732"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            145..1365
                     /locus_tag="VLB45_05485"
     CDS             145..1365
                     /locus_tag="VLB45_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007550051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HSD05188.1"
                     /translation="MAIESHFIQTIIGVGILLFAAKLMAELFHRLKLPIVLGELLAGM
                     IVGPFALGAFLVYDGQQLLKIDEELKILGEIGAIVILFIAGLEVTPKEFLRGGKASFM
                     VGACGVVVPFFVGFIIFGMFGFDAFQSMLIATALTATSIAISVQVLREFGKLQTPEAR
                     LIIGAAIVDDILAIAVLSVVSSLGAGGGEIINIDPLQIVIIILQVLGFFAALLIGSLI
                     IIPRIVARKVWKAEGSIEAIATASFFGAAALAGSIGLSPIVGAFAVGMALSSTKVIDK
                     IEQYISKLGIIFAPLFFAFIGAQVDFRGVNAEILMLSGIMISVAVVTKYLGCGLPAML
                     FLKDRRKSMQVGIGMISRGEVGLIVAGIGVASGVLTSSVYSTIVIMVAVTTIITPLWL
                     KASYKKESKPPAPI"
     gene            1435..1830
                     /locus_tag="VLB45_05490"
     CDS             1435..1830
                     /locus_tag="VLB45_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007550339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05189.1"
                     /translation="MSLDENLLSQIMEKQNQEYFLHYKNKTYVLENVTIAKFPMPVNK
                     PTTRGGVYSISDKEYKIKGTIHDTSLVPFISKMMLGPNAEFEEIQITTKILHEGKIKM
                     LTLFAYLTNAMNNSSKIEFNLLLVRTNIN"
     gene            1916..2203
                     /locus_tag="VLB45_05495"
     CDS             1916..2203
                     /locus_tag="VLB45_05495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05190.1"
                     /translation="MDATNGEIKKALVSLAVESTLLDMGRPVLEEVERSLYQNYRCYI
                     PDCYEHPEYLKNVLHDLYGASHSNIVKIIQKSLEEFSYQKPIENFLKIISE"
     gene            2206..2619
                     /locus_tag="VLB45_05500"
     CDS             2206..2619
                     /locus_tag="VLB45_05500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05191.1"
                     /translation="MKDIIKKSKDYEDDWVPIIDKSLTRLILRAFGDDDKEKILRVLA
                     KKPDMISSVLEICKIPKTSGYRKINSLIDDGLLLPVGLGRGVDGKKITQYVSAFENLQ
                     IEIVKNKVVVKVQIHKAVEDLPDSKIKDLANVTVN"
     gene            2644..3417
                     /locus_tag="VLB45_05505"
     CDS             2644..3417
                     /locus_tag="VLB45_05505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05192.1"
                     /translation="MQKFLFISLFVIALALVLANLYGKETAGQVSNILYLIVPGALLV
                     LSILISIRFGTKGNHAKAWIMFACFASSWFIAERMWMVYDLVYHIEPFPSWADLFYLA
                     GYPFLFIFAAYYIKPIKNISKNTLLVAFVFSTIMLSISMYLTSNADFGDDLISYALAA
                     SYPVFDSIILFPAVVGIILFFKGKVNFLWSLICSAIIINVFADVGFLFAQADDSFYTG
                     HPIEVLYLWSYALFSFGVYSHILIFKNHKKDPYKKDDLR"
     gene            3540..3707
                     /locus_tag="VLB45_05510"
     CDS             3540..3707
                     /locus_tag="VLB45_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007551696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05193.1"
                     /translation="MKEISEDSVFTDPQTREKESAKFLRHLTYNHSEVIKAVMNDLPT
                     QKRSQEFDWFR"
     gene            3785..6289
                     /locus_tag="VLB45_05515"
     CDS             3785..6289
                     /locus_tag="VLB45_05515"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HSD05194.1"
                     /translation="MKQISPKNYQITFEPNFKNFTFLGKEKISIELLKPTNTLVLNSA
                     KIDIKECLMTSKGKAFKTTITLDEKNEQLTIRIPEKIRGKAELYINFVGILNDKLVGL
                     YRSEYLDKNGKKKIMATTQFEAADARRAFPCWDEPEAKATFDISVIVEKNMTAISNMP
                     VISKKKIGNKTLFKFDRTPIMSTYLLYIGVGEFEFLTGKLGKTLIRIITTKGKKELGK
                     LSLEFTKQFLGWYEKYFKIPYPLPKLDMIAVPDFASGAMENWGAITFRETILLYDPKT
                     SSTETKQHIAEVISHEIAHQWFGNLVTMKWWNDLWLNESFATFMATKAVHHFYPEWDL
                     WNQFLKAETSGAMSLDALKTSHPIDVKVNKPSEVREIFDEISYNKGGSVLRMLEDFLG
                     EENFRKGLKNYLSKHKYGNATTEDLWNALEAVSRKPVRMMMNSWVRQVGYPVIDASLD
                     GSKLLLSQKRYLAENDGKSQKGTWVIPVSVKTQDRLVSKLMTKKSDTLSLGDPEWIKI
                     NHEQKGLYRVKYDDDMLQKLQRLVEEKALDNADRWGIQHDLFSLCISGQMSLKNFLDF
                     TRSYFDEDDYLVSADLAGGLDFLYLITSGEIFWDEIKEYNKEFFRKLYEKLSWDPQPG
                     EKHTTALLRSIVIGSLGRLDDEEVIEEANKRFQEFLKKPNSLRPDLRGTVYSLVAWSG
                     DEKTYDLLLKLYKKAQMQEEKLRFLGALCSFQDEKLLAKALRFALSRDVRSQHLHLPV
                     MRVAGNPYGRSLVWPWVKKNWKTIVKKFGVGSPLLNRVIGSTSAVYDGKKESEIRQFF
                     KKHPTPGTEMKLAQTLERIRIHSKFLNGARKEFLIN"
     gene            6369..6995
                     /locus_tag="VLB45_05520"
     CDS             6369..6995
                     /locus_tag="VLB45_05520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05195.1"
                     /translation="MTIKNKHAFGFTTMMSILAVFVLFAHNSAFGATSDSEPEIKATD
                     ELKKNPLIMKILKNIEISKKKIAEMQKKQLAINEHKKFLETQRKIANEKFQNDLARMN
                     KETADFTPKASYSKFLSGISETYHGIFWSQFNYQEQKVNLARAAMDNVLKNGGSYQDA
                     RQVYSKIGAMTKTEITNLLKDLNIQHGFAKEDTQKAFDKYGKLPRTRD"
     gene            7029..7256
                     /locus_tag="VLB45_05525"
     CDS             7029..7256
                     /locus_tag="VLB45_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05196.1"
                     /translation="MTHFIEDDDRERITLLEIVSKKGLRILTKEQLIRLQTLVENKNY
                     GNDKKTMKSKAKLLEKISFEIYNKDKGGFCV"
     gene            complement(7258..7392)
                     /locus_tag="VLB45_05530"
     CDS             complement(7258..7392)
                     /locus_tag="VLB45_05530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05197.1"
                     /translation="MSDTGKTREKKFFFDIEQLTKANEQFKNIAEIQKELRKKHDAKI
                     "
     gene            7543..7890
                     /locus_tag="VLB45_05535"
     CDS             7543..7890
                     /locus_tag="VLB45_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010189684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05198.1"
                     /translation="MKIIDVLNPKRIDREPDKTVTLLSEGKFVEQGIVVKKIMLCLYI
                     QKHDKKLGPYSLVTALIETDKGEIEMTYDEGYRGENALEEAAGFLTSNLGLSGLILRS
                     IIQLKAELEKPRK"
     gene            7887..8096
                     /locus_tag="VLB45_05540"
     CDS             7887..8096
                     /locus_tag="VLB45_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HSD05199.1"
                     /translation="MIYKVSVTFHKDFVQVNGDEITIGIKSEPQKGKANKELLEKISA
                     YFKVSKSNVRIVSGEKSRKKLVEVV"
     gene            8483..8956
                     /locus_tag="VLB45_05545"
     CDS             8483..8956
                     /locus_tag="VLB45_05545"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010192123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HSD05200.1"
                     /translation="MRAKSPNESKAEMIVRMFPSDANPAGNVFGGEILKQIDLVAGLV
                     AQRHAQTNTVTASIDRVNFLKPVYVGNALVLNARLNFVSRSSMEIEIKVESEDFVNNS
                     RTLTGTAFVTAVALDKEGKPTQVPELLLETDEDKTKFAEGKSRMEQRARERQKSK"
     gene            complement(9063..9251)
                     /locus_tag="VLB45_05550"
     CDS             complement(9063..9251)
                     /locus_tag="VLB45_05550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05201.1"
                     /translation="MNLIISYLLCTEFKKYRFDDLPVMCEMLEEIQAAQLAAVLARAR
                     KQRMESQYVEKPQETIAQ"
     gene            9289..>9770
                     /locus_tag="VLB45_05555"
     CDS             9289..>9770
                     /locus_tag="VLB45_05555"
                     /inference="COORDINATES: protein motif:HMM:NF024804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HSD05202.1"
                     /translation="MMQDRTSSKDPAPDLPIKAEEYLEVITTEDERIKIIGEELANDT
                     GRAIFGKISQGVSSPNDLAKALNISLPLINWHINRLLSVGLVKVEKTELSSKNKQVKY
                     YGPVKTALVIIPPEELTPNGGKSNAGKESVLLRLRQYLASIAAFVVGAPAIYAIQRSM
                     "
BASE COUNT         3387 a         1714 c         1882 g         2787 t
ORIGIN      
        1 atgaaaatag acgcctctca catgtaatat tggtgtcatt tgatacaaac gcggatgtcc
       61 ataatataat aacaaaaaaa ataattccat tggttcgcca aaacataagt aatatagcgt
      121 aaaaacctcg tatctaaaga agccatggca atagaatcgc attttattca aactataatt
      181 ggtgtaggaa ttctgctttt tgcagcaaaa ctaatggctg agctgtttca cagactgaaa
      241 cttccaatcg tattaggtga gcttttggca ggaatgatag tgggaccatt tgcacttggg
      301 gcgtttcttg tttatgatgg tcaacaactg ttgaaaatag atgaagagct aaaaatttta
      361 ggagaaattg gagcaatagt tattcttttc attgcaggac tagaagtaac accaaaagaa
      421 tttttgcgtg gaggaaaggc atcgtttatg gttggagcct gcggtgtagt tgttccattt
      481 tttgtaggat tcattatctt tggtatgttt ggctttgatg cgtttcaatc aatgcttatt
      541 gcaacagctt tgactgcaac aagcattgca atttcagttc aagtgttacg agaatttggc
      601 aagttgcaaa ctcctgaagc acgtctgata attggagcag ccattgtaga tgacattctt
      661 gctattgcag tcctatctgt agtgtcttca cttggtgccg gtggtggtga aatcataaac
      721 attgatccac ttcaaattgt gattataata ctgcaagtac ttggtttctt tgcagcacta
      781 ctcataggtt ctttaataat aattccaaga attgttgcaa gaaaggtctg gaaagcagag
      841 ggaagcattg aagcaatagc cactgcatcg ttttttggtg ctgctgcatt ggcaggatca
      901 ataggactct cgccaatagt tggtgcattt gccgtaggaa tggctctctc ctcaaccaaa
      961 gttattgaca aaatcgagca atatatcagc aaactaggaa taatctttgc accgttgttt
     1021 tttgcattta tcggagccca ggttgatttt aggggagtca atgcagaaat attgatgctt
     1081 agcggtataa tgatttcagt agcagtagtt acaaaatatc ttggatgtgg tttacctgca
     1141 atgttgtttt tgaaagacag gagaaaatca atgcaagttg gaatagggat gatttcaaga
     1201 ggcgaagttg ggctaattgt ggcaggaatt ggtgttgcct ctggagttct tacatctagt
     1261 gtctactcca caattgtgat catggttgca gtaactacta ttattactcc actgtggcta
     1321 aaagccagct acaaaaaaga atcaaaacct cctgcaccga tctaaatcta atcaaaagat
     1381 aatttgatca actgatggta agattcaata aaaacacaag gaaactaaat cttaatgtct
     1441 cttgatgaaa atcttctgtc acaaataatg gaaaaacaaa accaagaata ctttcttcat
     1501 tataaaaaca agacatatgt tttagaaaat gtaacaattg ctaaatttcc aatgccagta
     1561 aataagccga caacaagggg cggtgtttat tctataagtg acaaggaata caaaatcaaa
     1621 ggaaccatac atgacacttc ccttgtacca tttatctcca aaatgatgct tggtccaaat
     1681 gctgaatttg aagaaattca gatcacaacc aaaatactgc atgaaggaaa aatcaagatg
     1741 cttaccttat ttgcttattt gactaatgcc atgaataatt catcaaaaat tgaatttaat
     1801 ttactacttg tgaggacaaa tatcaattaa tatgctcttt tgtgattcat gacgcagggt
     1861 atcttttccc ggagttggat atttgtttta ataaccccag tgaaacccta tcttgttgga
     1921 cgctactaat ggtgaaataa aaaaagcact agtatcacta gctgtggaaa gtacactact
     1981 tgacatgggc aggccagtgc tagaagaagt tgaacgtagt ctgtatcaaa attatcgatg
     2041 ctacattcct gattgttatg aacatcctga atacttgaaa aatgtattac atgatctata
     2101 tggagcatca cattcaaata ttgtaaaaat cattcaaaaa agtcttgaag aattttctta
     2161 tcaaaaacca attgagaatt ttcttaaaat aataagtgaa taaacatgaa agacattata
     2221 aaaaaatcta aagattatga agatgactgg gttcctataa tagacaaatc tcttaccaga
     2281 ctaatcctgc gtgcattcgg ggacgatgac aaggaaaaga tcttgagagt tttggcaaaa
     2341 aaacctgata tgatttcaag tgtactcgaa atttgtaaaa ttccaaaaac ttctggttat
     2401 agaaaaataa attctttgat tgatgatgga ttattgttgc ctgtcggtct tggtcgtgga
     2461 gtagatggaa aaaaaatcac ccagtatgta tctgcatttg aaaatctaca aattgaaatt
     2521 gtcaaaaata aggtagttgt caaagtgcaa atacacaagg ccgttgaaga tttaccagat
     2581 tcaaaaataa aagatctcgc taatgtcacc gtaaattaac tgtgacttga ttttgcaagc
     2641 gtcatgcaaa aatttctttt catatctctt tttgtaattg cactcgcact agtacttgca
     2701 aatctttatg gaaaagaaac tgctggtcag gtatcaaaca tactgtacct tatagtgcct
     2761 ggagcgctac ttgttttatc catattaata tcaattagat ttggtacaaa aggtaatcac
     2821 gcaaaagcct ggataatgtt tgcgtgtttt gcatcatcat ggtttattgc agaaagaatg
     2881 tggatggtat acgatcttgt ttatcacata gagccctttc cttcttgggc tgacttgttt
     2941 tatcttgcag gatatccgtt tttattcata tttgcagcgt attatataaa accaataaag
     3001 aatatttcaa aaaataccct gctggttgct tttgtctttt ctacaattat gctctccatc
     3061 tcgatgtatc ttacatcaaa tgctgacttt ggggatgatt tgatttccta tgcgttggca
     3121 gcatcgtatc ctgtctttga ttcaataatc ctattccctg cagtggttgg aattatttta
     3181 ttttttaaag gcaaggtaaa ttttttgtgg tcgctgatct gctctgcaat aataatcaat
     3241 gtgtttgcag acgttggttt cctatttgca caagcagatg attctttcta tactggccat
     3301 ccaattgagg tattgtatct gtggtcgtat gccttgtttt cctttggtgt gtatagtcac
     3361 attctgatat tcaaaaacca taaaaaagat ccttacaaaa aagatgatct aagataaaat
     3421 caaattcagg cacaaattag atcgcggaca agaccatatt tattaaactg aaacaattac
     3481 acaatgatat attgaaactt tatcttgatt tcgaaccatg tgatgaatgt cgtgttatta
     3541 tgaaagagat tagtgaagat tctgttttta cagatccgca aacaagagaa aaagaatccg
     3601 caaaatttct gcgacatctc acctataatc atagtgaagt aatcaaagca gtaatgaatg
     3661 atttgccaac acaaaagcga agccaagaat ttgactggtt tcgataaatt gcaaaataga
     3721 taaaaattaa tcaatcttgt agctgcgata gacttactta gcccaacaaa ttaaacaaac
     3781 cttcatgaag caaatctctc caaagaacta tcagattaca ttcgaaccaa atttcaaaaa
     3841 tttcactttt ctaggcaagg aaaagatatc tatcgaactt ttaaaaccaa caaatactct
     3901 ggtgctgaac tctgcaaaaa tcgatataaa agaatgtctt atgacctcaa aaggaaaagc
     3961 attcaagact acaataacac tagatgaaaa aaatgaacaa ctgacaatcc gtatacctga
     4021 gaagatcagg ggcaaggcag aactttacat caattttgta ggtatactaa acgacaagct
     4081 tgtaggattg tacagaagcg agtatcttga caagaacgga aaaaagaaaa tcatggcaac
     4141 tacacaattt gaggctgcag atgcaagacg tgcatttcca tgctgggatg aacctgaagc
     4201 caaagcgacg tttgatattt cggtgattgt agaaaaaaat atgactgcca tatccaacat
     4261 gccagtcatt tcaaagaaaa aaatcggcaa taaaacgctc ttcaagtttg accgaactcc
     4321 gatcatgtct acatatctat tgtacattgg agttggcgaa tttgaattct tgacaggcaa
     4381 gcttggaaaa acactgatcc gaatcatcac aacaaaaggt aaaaaagagc taggaaaact
     4441 gtctctagaa tttacaaaac aattcctagg ctggtacgaa aaatacttca aaattccata
     4501 ccctcttcca aaactagaca tgattgctgt tccagacttt gcctcagggg caatggaaaa
     4561 ctggggtgcc atcacgttta gagaaaccat acttctttat gatcctaaaa catcatctac
     4621 tgaaacaaaa cagcacattg ctgaagtaat atcacatgag attgcccacc agtggtttgg
     4681 taatcttgtg acaatgaagt ggtggaatga tctgtggtta aacgagagct ttgcaacgtt
     4741 catggccaca aaagcagtac accactttta tccagagtgg gatttgtgga accagtttct
     4801 caaagcagaa actagtgggg ctatgagcct tgatgcatta aaaacatctc atccaattga
     4861 tgtcaaagta aacaaaccct ctgaagttag agaaattttt gatgagatta gctacaacaa
     4921 gggcggctct gtacttagga tgcttgaaga ctttttggga gaagagaatt ttcgcaaggg
     4981 tctgaaaaac tatctttcaa agcacaagta tggtaatgct acaaccgaag acctctggaa
     5041 tgcacttgag gctgtttcaa gaaagccagt tagaatgatg atgaacagct gggtacgaca
     5101 agtaggttat ccggtaatag atgccagttt ggatggttcc aaattgttgc tgtcccaaaa
     5161 gcgatacttg gcagaaaatg atggcaaatc acaaaaagga acttgggtaa tccctgtatc
     5221 tgttaaaaca caagaccgcc ttgtttcaaa gctgatgaca aagaaatctg atacgttgtc
     5281 gttgggtgat cctgaatgga ttaaaataaa tcatgagcaa aaggggctct acagagtaaa
     5341 atatgatgac gatatgttac aaaaactcca aagactagta gaagaaaaag cactagacaa
     5401 tgcagacagg tggggaatac aacacgatct gttttcattg tgcatatcgg gccaaatgtc
     5461 actaaagaac ttccttgatt ttacaagatc atattttgat gaggatgatt atcttgtttc
     5521 tgcagattta gcaggaggtc ttgactttct ctaccttata acatctggag aaatcttctg
     5581 ggatgaaata aaagaataca acaaagaatt tttcagaaaa ctctatgaaa agcttagctg
     5641 ggatccgcaa ccaggtgaaa agcataccac tgcactactt cgtagcattg tgataggttc
     5701 acttggcaga ctagatgatg aagaggtaat tgaagaagca aacaaaagat tccaagaatt
     5761 cctcaaaaaa ccaaactccc tcagaccaga cttgcgtgga acagtatatt cacttgtagc
     5821 ttggagtgga gatgagaaaa cctatgatct gttactaaaa ctctacaaaa aggctcagat
     5881 gcaagaagag aagcttagat tccttggggc attgtgtagc tttcaggatg aaaaattgct
     5941 tgcaaaggca ctacgatttg ccctctcacg agacgttcgt tctcagcacc tgcatctacc
     6001 agtaatgagg gtggcaggaa acccatatgg aagaagcttg gtatggccat gggtaaagaa
     6061 aaactggaaa accatagtca aaaagtttgg tgtaggcagc cctcttctga acagggttat
     6121 tggcagcacc tctgcagtat atgatggaaa aaaagaatca gaaataagac aattcttcaa
     6181 gaaacatcca acgccaggaa cagaaatgaa gcttgctcaa actctggaaa gaataagaat
     6241 ccattcaaaa tttttgaatg gggcacgaaa agaattcttg attaattaga tcgtgcattt
     6301 tatactaata agacttgcta accatttcta cgtaatcagt aaatactgtt tttgattcca
     6361 gagaatcaat gactatcaaa aacaaacacg cgtttggttt cacaaccatg atgtctattc
     6421 tggcggtgtt tgttttattt gcacacaatt ctgcgtttgg ggcaacatct gacagtgaac
     6481 ccgaaataaa agcaactgat gagctaaaaa agaacccgtt gataatgaag attctaaaaa
     6541 acattgaaat ttctaaaaag aaaatcgcag aaatgcaaaa aaaacagtta gccattaatg
     6601 aacacaaaaa attccttgag acacaaagaa agattgcaaa tgaaaaattt caaaatgatc
     6661 ttgcgcgcat gaataaggaa actgcagatt ttacacctaa agcatcttac tccaaattcc
     6721 tttcaggaat aagtgaaact tatcatggca tattttggag ccaattcaat tatcaagaac
     6781 aaaaagtcaa tcttgcacgt gcagcaatgg ataacgttct aaaaaatggc ggtagctatc
     6841 aagatgcaag gcaggtgtac tcaaagattg gtgccatgac taagactgaa atcaccaatc
     6901 ttctcaaaga cctaaacata caacatggat ttgccaaaga agacacacaa aaagcctttg
     6961 acaagtatgg caagcttcca agaaccagag attaacctaa attaaaccca aaagcctaat
     7021 caaaacttgt gacacatttt atcgaagatg acgatagaga aagaataaca ctgctggaaa
     7081 ttgtatcaaa gaaaggactg cgcattttaa caaaggagca gctgatacgc cttcaaaccc
     7141 tagtagagaa caaaaactat gggaatgata aaaaaactat gaaatcaaaa gcaaagctgc
     7201 ttgagaaaat ctctttcgaa atatacaata aagataaggg aggattctgt gtctgaatta
     7261 tatcttggca tcatgttttt ttcttagctc cttttgaatt tctgcaatat ttttaaattg
     7321 ttcatttgct ttcgtcagtt gctctatgtc aaaaaagaac tttttttctc tagttttacc
     7381 tgtgtctgac attatgccat ttctcctagg tatacaataa aaccactaga caaattcaca
     7441 tcaaactgta tcaaagcatc tagttttaaa atttactttg tgattctatt tgtcttgtta
     7501 tattgtaaaa gcaaatctag ctgtgatgat attttgtaag tgatgaaaat cattgatgtg
     7561 ctcaatccca aaagaataga cagagaacca gacaaaactg taacattact gtcggaagga
     7621 aaatttgtag aacaaggcat agtagtaaaa aaaatcatgc tctgtttata catccaaaaa
     7681 catgacaaaa aactaggccc ttattcgctt gttactgcct tgattgagac agacaagggc
     7741 gagattgaga tgacttatga tgagggctat agaggtgaaa atgcgcttga agaggcggca
     7801 gggtttctta cttccaatct tggtctatct ggtctgattt tacgttctat aatacaactg
     7861 aaagcagaat tggagaaacc tcgaaaatga tatacaaggt ttcagtaact ttccataaag
     7921 attttgttca agtaaacggg gatgaaataa caattggcat aaagtcagaa ccgcagaagg
     7981 gaaaagccaa caaggaatta ctggaaaaaa tttctgcgta tttcaaagta tcaaaatcca
     8041 atgtcagaat agtgtctgga gaaaaatcta gaaaaaaact agtcgaagtt gtctaaaaaa
     8101 acactactcg tatgcgtaac ttggatgcat tgatcctacg caattttgat ttcatatgta
     8161 atcagtattg caatcaagac agtgtatctt gggtgcaatc atttctcctg acaagtagac
     8221 ttctaatgat ccgcatcctg ggcacatgaa cctaaagtca ggataatgtg gcttcaaata
     8281 ggatctatct tttttcaaaa gcgctcttac aatttttgct ctttgaacta tttggtagta
     8341 ttgtaccatg tatgtgaaat cgtgaaaata gaacataaag aacgcgtagc atttgttcag
     8401 gatgactttg tagcaacgct taaaatgtaa tttctgatct gaatcgaatt ctatttatta
     8461 agaaaacgta tagttgcaaa tcttgagagc aaaaagtcca aacgaatcaa aagcagagat
     8521 gattgtacgc atgtttccat ctgatgcaaa tcctgcagga aacgtctttg ggggcgagat
     8581 tctaaaacaa atcgaccttg tggcaggtct tgtggcacaa agacacgcac aaacaaacac
     8641 agtaactgca agtattgaca gggtgaattt tctaaaacca gtatatgttg gaaacgcact
     8701 tgtactaaat gcaagactaa actttgttag tcgctcatct atggaaattg agatcaaagt
     8761 ggagtctgaa gattttgtta ataattctag aaccctaact ggtactgcgt ttgtgaccgc
     8821 agtagcactg gataaagaag gcaagccgac acaagttccg gaattgctgc ttgagactga
     8881 cgaagacaag acaaagtttg cagaaggaaa atcaaggatg gagcaacgcg caagagagcg
     8941 ccaaaaaagc aagtaaactc attttgtttg atgcttgtaa caagtacagt gctttgacca
     9001 gtgcttagca cagcctaaca ccatatgatc tttgcaaccg caaataacgc attttttcat
     9061 gtttactgtg ctatggtttc ttgaggtttt tctacgtatt gactttccat tctttgttta
     9121 cgggcacgag ccaatactgc tgctagctgg gcagcctgaa tctcttctag catttcgcac
     9181 atgactggta aatcatcaaa cctgtacttc ttaaactctg tgcataaaag atacgaaatt
     9241 atcaaattca ttctgaacct attttaaacg gaagctgaat attgaaaaat gatgcaagat
     9301 agaacctcaa gtaaggatcc agcacctgat ctaccaataa aggcagaaga atatcttgaa
     9361 gtgatcacta ccgaggacga acgaatcaag ataataggtg aagagctagc aaatgatacg
     9421 ggtagggcaa tatttggaaa aatctcacaa ggggtatcta gcccaaacga tcttgcaaag
     9481 gcgttaaaca tttcacttcc actaattaat tggcatatca accggctcct aagtgtagga
     9541 ctggtcaaag tagaaaaaac tgaactaagc tcaaaaaaca agcaagtgaa atactatggg
     9601 cctgtcaaga cagccttagt aatcattcct ccagaagaac taactcctaa cgggggaaaa
     9661 tctaatgcag gaaaagaatc cgtgttgcta agactcagac agtatcttgc aagcattgca
     9721 gcttttgttg ttggtgctcc agccatttat gcaatacaaa gatcaatgag
//
LOCUS       DATIDL010000127         7607 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrosopumilaceae archaeon isolate SMAG_U16342
            k141_2583960, whole genome shotgun sequence.
ACCESSION   DATIDL010000127 DATIDL010000000
VERSION     DATIDL010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935836
            Sequence Read Archive: SRR25158326
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosopumilaceae archaeon (soil metagenome)
  ORGANISM  Nitrosopumilaceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrosopumilales;
            Nitrosopumilaceae.
REFERENCE   1  (bases 1 to 7607)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7607)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/04/2024 05:09:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,520
            CDSs (total)                 :: 1,474
            Genes (coding)               :: 1,474
            CDSs (with protein)          :: 1,474
            Genes (RNA)                  :: 46
            rRNAs                        :: 1 (5S)
            complete rRNAs               :: 1 (5S)
            tRNAs                        :: 43
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7607
                     /organism="Nitrosopumilaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2583960"
                     /isolate="SMAG_U16342"
                     /isolation_source="soil"
                     /db_xref="taxon:2202732"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..527)
                     /locus_tag="VLB45_05560"
     CDS             complement(<1..527)
                     /locus_tag="VLB45_05560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HSD05203.1"
                     /translation="MGKKIRIAIAGIGNCASALIQGAHYYSKNPSDIVGLTAYNIGGY
                     EPGDIEVVAVFDVADTKVGKDVSEAIYARPNNTVTVAEVPKMGVTVQKGPTLDGIGRH
                     LSQIVTPSKEPDVDVKKILKDTGAEMLVNYLPVGSTTAVRYYATQALEAGIGFINAMP
                     VFIASESKWQQMFAD"
     gene            628..1566
                     /locus_tag="VLB45_05565"
     CDS             628..1566
                     /locus_tag="VLB45_05565"
                     /EC_number="2.5.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase"
                     /protein_id="HSD05204.1"
                     /translation="MIKPGRPVEDISIKKGDSIQNIFDEMSKSGGFESRNLVEGLDIL
                     STMIQDKNCLKFLSFVGAVTSTGLRGIIKDMIKNKMFDVVITTCGALDHDIARYFSKY
                     LEGSFTLDDNELADKHIHRLGNVLVPMESYGPLIEEKMQAFLEKIYRNGKKEMSTAEI
                     TKMIGENLGEGSFLYWASKNNIPVIVPGIMDGAVGSQIWLFTQKHGDFKLNIAADGDL
                     LSGLIFKAKKSGALMIGGGISKHHTLWWNQYRDGLDYAVYITTAQEFDGSLSGALVRE
                     AISWGKVTQKAMQTTIHAEATTILPFLYSALLSKLK"
     gene            complement(1559..2203)
                     /locus_tag="VLB45_05570"
     CDS             complement(1559..2203)
                     /locus_tag="VLB45_05570"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HSD05205.1"
                     /translation="MECGTLFANEKLPNDIFFDKLTNITCLSEKMIDEAIALFKKYNT
                     QPYVYVLDNPDLEKMLVKKGFVLYDTQHVLTKNSDSNLEHSAMHHVAKKDSLLWAEIF
                     CKSYDCLEWIDEVNSIVRNSVSQIEYLVDSEHNASCVALYEKNSILGLYCLGTIPEYR
                     KRGLGKSLINYALTQVKRKNLDFLMLETYEKDNLIEFYSNLGFEKIYEKKVYSI"
     gene            complement(2263..3423)
                     /locus_tag="VLB45_05575"
     CDS             complement(2263..3423)
                     /locus_tag="VLB45_05575"
                     /inference="COORDINATES: protein motif:HMM:NF013565.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HSD05206.1"
                     /translation="MIKLFNSFSLKKEDFEPFDKKRVKIFICGPTLYDYTHLGHARIF
                     LTYDLLCRYLKGKNYAIDVLVNMTDINQSVFNKALDQGTDYFTLTRFYSGEFVKDLSL
                     LNITSITRLAHVSDYVKNILQNISSLVKTKNAYSANGNIYFDTKTAKGYGKISKQTRE
                     QLNLHRMDIGPNKKNPEDFMLWNCSEDFGFSWKGDFGNGIPWWHIQDTAVAFENFGSH
                     YDIHGGAKELAYPHHEAHLAQYQALTGMENSVQRWMYVGLVLVEGEKMSKSLGNMVLA
                     RNLVEKYGQNLLRLYLFSTHYREDLNYKEEDLVAKKKFLEKIYKVYNKTSDKTSADVS
                     KLINEFLDALEDDLNSPKALQIFERICDVVLQSSNISTLDFEKISGTIGLSV"
     gene            complement(3432..3677)
                     /locus_tag="VLB45_05580"
     CDS             complement(3432..3677)
                     /locus_tag="VLB45_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007550931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HSD05207.1"
                     /translation="METLYILINCDLGSEVDIINEFMKIPEVKEVRGTYGVYDIFVKL
                     QGDSKETLENIITHKIRRIPKIRSTVTLTPILAQGGR"
     gene            3748..4461
                     /locus_tag="VLB45_05585"
     CDS             3748..4461
                     /locus_tag="VLB45_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAC2 family protein"
                     /protein_id="HSD05208.1"
                     /translation="MGSEITIREIKPINIEGGYLIDGFPYVGLANAIATESLINTVQF
                     ETVGVIDSDLFPPISIVKDGVPNFPARIFVNSDLKVAVFSSYLTPHESIHRLVAKSML
                     EWANKHKCSLIVSSSAIKTDDENSFIIGVGSTEAAKKKLKDAGIPMLSHGTIPGIPGI
                     LLNEGTLSNTNVIVLLFNSRERGPDFKSGAEICMAMSKLVPGAACDLNLLLQEAQTAE
                     QSIKKTEEDAEPLKDAMYS"
     gene            4458..4706
                     /locus_tag="VLB45_05590"
     CDS             4458..4706
                     /locus_tag="VLB45_05590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05209.1"
                     /translation="MTYQEKEITKNQLSRFIQVLDLDEGIRIENKTNMIFLNKSAKRY
                     CINTATKEKEEFVYRNTASDVLDFLKDKIDEGCKIFSY"
     gene            4737..5345
                     /locus_tag="VLB45_05595"
     CDS             4737..5345
                     /locus_tag="VLB45_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007401360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HSD05210.1"
                     /translation="MLEFAITVIIISISGVMSPGPLFAANISYGLKDGIKAGLKMAYG
                     HTVVELPLVVFLGMGILSLETIPQFRETISLVGAISLFVFSGLQIKSVVQKPTTVFAG
                     RHGPFIAGIMLSALNPFFLIWWFTIGSKLISDALYLASFAGIGIMFAFHIWMDYAWLC
                     AVAFLSSKGKKIITNKRYTIFMIAISGVLVYYGIVFALEVLG"
     gene            complement(5342..5581)
                     /locus_tag="VLB45_05600"
     CDS             complement(5342..5581)
                     /locus_tag="VLB45_05600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05211.1"
                     /translation="MTSTFLKRAIIISLCGIAAIVVLYGIGLSNVCPIKHVQVVDELK
                     KYQKTLDPELCEALVEKIIDLNNQCGIEIEVIDCG"
     gene            complement(5583..5861)
                     /locus_tag="VLB45_05605"
     CDS             complement(5583..5861)
                     /locus_tag="VLB45_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HSD05212.1"
                     /translation="MSRPPNVIFIGKKSIMTYVNATLTLLANEPVVTIKARGKSITTA
                     VDVSQMIVKRLNAVGYKVSDVRIFSERMTSQDGRERNVSTIEIDVSRP"
     gene            complement(5886..6143)
                     /locus_tag="VLB45_05610"
     CDS             complement(5886..6143)
                     /locus_tag="VLB45_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05213.1"
                     /translation="MWILDYPVIAEPEGVKIKPEKMEADKLYHCVFKDKVLLFYKDQN
                     DVLNCYEIEEKELVDSIKQSESPEQVENILKKFIEKENLNH"
     gene            6213..7307
                     /gene="dinB"
                     /locus_tag="VLB45_05615"
     CDS             6213..7307
                     /gene="dinB"
                     /locus_tag="VLB45_05615"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HSD05214.1"
                     /translation="MEQRIVFHIDFDYFFAQCEELRNPKLKTVPVVVCVFSERGGDSG
                     AIATANYLARKYGVKSGMPIKFAKKKLESVSDAVFLPTDFDYYSDISQKAMDIIKEHA
                     DIFEYVGRDEAYLDVSKKTEYSFVDAGHLAQQLKNTLRNSIKLSCTIGVSSNKLVAKI
                     ASDFKKPDGLTIVTPDKIESFLNPLPIRSIPGIGKKTEERFAEMNLETISQLKTLDVF
                     TLNSEFGRKIGAYIYNAARGIDNDPVAPRQEATQYSRITTLKRDSNEYDFLAKDLERL
                     CEDIHATILKDNIVFKSVGIQFVQVDLSNKTRSKTLKNSTSNLDELKRAALQLLRESL
                     EDQKMLVRRIGVKVSELSEVTGQDNITRYF"
     gene            complement(7304..>7607)
                     /locus_tag="VLB45_05620"
     CDS             complement(7304..>7607)
                     /locus_tag="VLB45_05620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05215.1"
                     /translation="NEDATIVIGYDHLKKSDSKILYLDIVHELVHVKQQRSGLDLYDK
                     SYSYVDRPTEVEAYEVAVREAKRIGMNQQEIFDYLHVEWITPDEHKRLASRLGISV"
BASE COUNT         2431 a         1385 c         1358 g         2433 t
ORIGIN      
        1 ttgtcagcaa acatttgctg ccattttgat tctgatgcta taaacacagg cattgcattg
       61 ataaaaccaa ttcctgcttc aagagcttgc gtagcataat atcttacggc agttgtgctg
      121 ccaactggaa gataatttac aagcatttct gcaccagtgt cttttagaat ctttttaaca
      181 tcaacatctg gctcttttga aggagtaaca atttggctca ggtgccttcc tattccatca
      241 agagttggac ctttttgtac agtgactccc atcttgggaa cctcagcaac tgtaacggtg
      301 ttgtttggtc ttgcgtaaat ggcctctgag acatcttttc ctacctttgt atctgctacg
      361 tcaaagacgg caacaacttc aatgtctcct ggttcataac cgcctatatt gtacgcagta
      421 agtcctacta tatcgctagg attttttgaa taataatgag caccctgaat cagggcagaa
      481 gcacagttac caatacctgc tattgcaatt ctaatctttt ttcccaaagt aattcgatat
      541 ttacgcaaat tattccatca tattaattac ttgtgattac aatactgggt gcagaggatt
      601 ttataacccg atcttaattc atggtttgtg ataaaaccag gcagaccggt agaggatatt
      661 tcaataaaaa aaggcgacag catacagaac atatttgacg aaatgtcaaa gtcaggagga
      721 tttgaatcaa gaaatctagt ggaagggcta gacatacttt ctacaatgat tcaagacaaa
      781 aattgtttaa agtttctatc ttttgttggg gcagtgacat caaccggatt aagaggaata
      841 ataaaagaca tgataaaaaa caagatgttt gatgttgtga taactacatg cggagctttg
      901 gatcatgaca ttgcaagata tttctcaaaa tacttagaag gttcgttcac acttgatgac
      961 aatgagcttg cagacaagca catacatcga ttaggcaatg tacttgttcc aatggaaagt
     1021 tacggtccac taattgaaga aaagatgcaa gcatttcttg agaagatcta caggaatgga
     1081 aaaaaagaaa tgtcaacagc agagattaca aaaatgattg gagaaaattt gggggaaggt
     1141 tcatttcttt attgggcatc aaaaaacaac attccagtaa ttgttcctgg aataatggat
     1201 ggagcagttg gtagtcagat ttggcttttt acccaaaaac acggcgattt taagctaaat
     1261 atagcagcag acggagattt gttgtcaggt ctcatattca aggcaaaaaa atctggtgct
     1321 ctcatgattg gtggaggaat atcaaaacac cacaccttat ggtggaatca gtaccgtgat
     1381 ggtttggact atgccgtgta tatcacaaca gctcaagagt tcgatggtag tctgagtgga
     1441 gcacttgtga gagaagcaat ctcatggggc aaagtcacac aaaaagcaat gcaaaccacc
     1501 atacatgcag aagcgacaac catacttcct tttctttatt ccgcactgct ttcaaaactc
     1561 aaatagaata gactttcttt tcataaattt tttcaaaacc aaggtttgaa taaaattcaa
     1621 ttagattatc tttttcatat gtttcaagca taagaaaatc aagatttttt cttttgactt
     1681 gggttagtgc gtaattaatt aatgatttac caagccctct tttcctatat tcaggtatag
     1741 ttccaagaca atacaacccc agtatggaat ttttttcata taacgcaaca catgacgcat
     1801 tatgttccga gtccacaaga tactctattt gtgaaacaga gtttctgaca atagaattta
     1861 cttcgtcaat ccattcaaga caatcatatg acttgcaaaa aatctctgcc cacaacaatg
     1921 aatctttttt tgcaacatga tgcattgcag agtgttcaag atttgaatcg gaattttttg
     1981 tgagaacgtg ttgtgtatca tataatacaa atcctttttt gacaagcatt ttctcaagat
     2041 cgggattatc cagcacgtaa acatatggtt gcgtgttgta ttttttaaaa agcgctattg
     2101 cctcatcaat catcttttca cttagacatg tgatgtttgt gagcttatca aaaaatatat
     2161 catttggaag tttttcattg gcaaataaag taccgcactc caattgaatg gatttgctcc
     2221 acatgctagt aaatgctaga tcgacttctt cgatttgcga aatcatacgg acaagcctat
     2281 tgtgccagaa atcttttcaa aatcaagcgt gctgatgttg ctgctttgta aaacaacatc
     2341 acatattctc tcaaagattt gtaatgcttt tggcgaattc aaatcatctt caagtgcatc
     2401 tagaaattca tttatcaatt tagaaacatc tgcagatgtc ttgtcagatg ttttgttgta
     2461 aactttgtag attttttcta aaaatttctt tttagctaca agatcttctt ctttgtaatt
     2521 tagatcttct cggtaatgag tagaaaaaag gtacagtcgt aacaagtttt gtccgtattt
     2581 ttcaacaagg tttcttgcaa gaaccatatt cccaagagat tttgacattt tctctccttc
     2641 taccagtacc aatccaacat acatccatcg ctgaacggaa ttttccatgc cagtcagcgc
     2701 ttgatattgt gcaaggtgcg cttcatgatg tggatatgca agttcttttg cacctccatg
     2761 tatgtcatag tgagaaccaa agttttcaaa tgctacagca gtgtcctgta tatgccacca
     2821 cggaatacca tttccaaaat ctcctttcca tgagaatcca aagtcttcag aacaattcca
     2881 aagcataaaa tcctcaggat ttttcttatt tgggccaata tccatcctgt gcaggtttag
     2941 ttgctctctt gtctgttttg agatttttcc ataacctttg gcagtttttg tgtcaaaata
     3001 gatatttccg tttgcagaat atgcgttttt agtttttaca agtgatgaaa tgttttggag
     3061 tatatttttt acataatcag aaacatgagc aagccttgtg atgcttgtaa tgtttaataa
     3121 agaaaggtct ttgacgaatt cccccgaata aaatctcgta agtgtaaaat aatcggtacc
     3181 ttgatcaagg gccttgttaa acacgctttg attaatgtca gtcatgttta caagaacatc
     3241 aatggcatag tttttgcctt ttagatatct gcaaagcagg tcatatgtta gaaatattct
     3301 tgcatgcccc agatgagtat aatcatatag cgtgggtcca cagatgaata tttttacacg
     3361 ctttttatca aacggttcaa aatcttcttt ttttaaagaa aaagaattga acagtttaat
     3421 catttttggg tctatcgtcc accttgtgca agaatcgggg tcagtgtaac agtagaacga
     3481 atctttggta ttcttctaat cttatgagta atgatgtttt ccaaagtttc tttgctgtca
     3541 ccttggagtt tgacaaatat gtcatagacg ccataagttc ctcgcacttc ttttacttcg
     3601 ggaattttca tgaactcatt tattatatcc acttctgaac ccagatcaca atttatgagg
     3661 atgtataatg tttccacaat tttgctctat tattaccaca taaaatcata tccatgattt
     3721 aataggatgc cgcacatagc aaaattattg ggttctgaga tcacaataag agagatcaaa
     3781 ccgatcaaca tagaaggcgg ttatctaata gacggttttc cttatgtagg acttgctaac
     3841 gcaatagcta cagaatcttt gattaataca gtgcagtttg agacggtagg agttattgat
     3901 tctgatcttt tccccccaat tagcattgta aaagacgggg ttcctaattt tccagccagg
     3961 atatttgtaa attcagatct caaggtagca gtattttcat catatcttac tcctcatgaa
     4021 tctatacaca ggcttgttgc aaaatccatg ttagagtggg caaacaagca caagtgctct
     4081 ctgattgtca gcagctctgc aataaaaact gatgatgaga actcgtttat cataggagta
     4141 ggcagtactg aagctgccaa aaagaaacta aaagatgcag gtatacccat gctatcacat
     4201 ggaacaatac ctggaatacc cgggatttta cttaatgaag gcactttatc aaacacaaat
     4261 gtaatagtat tattgtttaa ttctagagaa agaggccctg attttaaatc aggtgcggaa
     4321 atttgcatgg caatgtcaaa actagttcca ggtgcagctt gtgatctgaa tctactgttg
     4381 caagaagcac aaacagcaga acagagtatt aaaaaaacag aagaagatgc agagccatta
     4441 aaggatgcaa tgtatagttg acataccaag aaaaggaaat aacaaaaaac cagctatcac
     4501 gatttattca agttcttgat ctggatgaag gaatcagaat agaaaacaag accaacatga
     4561 tatttctaaa taaatccgcc aagagatatt gcataaacac cgctaccaaa gaaaaggagg
     4621 agtttgttta tagaaatacc gcttcagatg ttttagattt cttaaaagat aaaatcgatg
     4681 aaggatgcaa aatcttttcc tattaataca aatattttcc acttgaggtg ctagaggtgt
     4741 tagaatttgc cataacagtc atcataattt ctatttcagg agtaatgtct ccaggcccac
     4801 tttttgctgc aaacatatca tacggactaa aagatggtat aaaggcgggt ttgaagatgg
     4861 catatggtca cacggttgta gaattgccat tggtagtttt tctgggaatg ggaatacttt
     4921 cacttgagac aattcctcaa tttagagaaa cgatatcact tgttggcgca atcagccttt
     4981 ttgtattttc tggactgcaa ataaaatcag tcgtacaaaa accaacaaca gtttttgcag
     5041 gaagacatgg tccctttatt gcaggaataa tgcttagtgc attaaatcca ttttttttaa
     5101 tctggtggtt cacaatagga tccaagttaa tttctgatgc gctttatctt gcctcgtttg
     5161 caggcatagg aatcatgttt gcatttcaca tatggatgga ttatgcttgg ctttgtgcag
     5221 tagcatttct ttcttcaaaa ggcaaaaaaa ttatcacaaa taagcgatat acaattttta
     5281 tgattgcaat tagtggcgtt ctagtctatt atggaattgt gtttgctttg gaagttctag
     5341 gttagccaca atcaattact tcaatttcta taccacattg attgtttaaa tcaataattt
     5401 tctcaacaag ggcctcacaa agttcagggt ccaatgtttt ttgatatttt tttaattcat
     5461 ctacaacttg aacatgcttt attggacata cattagataa tccaatacca tacaaaacaa
     5521 caattgccgc aattccacaa agagatataa ttattgcacg tttgagaaag gtacttgtca
     5581 tctcatggac gagaaacatc gatctctatc gtagaaacgt ttctttccct gccgtcttgt
     5641 gaggtcattc tttctgaaaa tattctcaca tcacttacct tgtaacccac tgcgtttagt
     5701 cttttcacta tcatttgaga gacatccaca gccgtggtta tgcttttacc tctggccttt
     5761 attgtgacta ctggttcgtt tgcaagcaaa gtaagagttg cattgacata tgtcattatg
     5821 gattttttac caatgaaaat tacgtttgga ggacggctca cagcccctct cgattcgcgt
     5881 tttatttaat ggttaagatt ttctttttct ataaatttct tcagtatgtt ttctacctgt
     5941 tctggtgatt cagactgttt tatagaatcc accagttctt tttcctcaat ttcataacag
     6001 tttagtacat cgttttgatc cttgtaaaaa agtaaaactt tgtctttgaa aacacagtga
     6061 tatagcttgt ctgcctccat tttttcaggt tttatcttta ccccttcggg ttctgcaata
     6121 acaggataat ccaatatcca cacccatgtg tggatgatat ttagattatt gtattagata
     6181 atgtaataag catttttttg ctttaaaatg taatggaaca aagaattgtc tttcacatag
     6241 attttgatta tttttttgca cagtgcgaag aattacgcaa tccaaaacta aaaacagtac
     6301 ctgttgtggt gtgtgtcttc tcagaacgtg gaggtgatag tggagcaatt gcgactgcaa
     6361 attatttggc aagaaaatat ggcgtcaaat caggcatgcc aataaaattt gccaagaaaa
     6421 aactagaaag tgtttcagat gcggtctttt tgcctactga ttttgattac tattcagaca
     6481 tatcacaaaa agcaatggac atcataaaag aacatgcaga tattttcgaa tatgtaggaa
     6541 gagatgaggc atacctagat gtctcaaaaa agacagagta tagttttgtg gatgcaggcc
     6601 atttggctca acaattaaaa aatactctgc ggaattccat aaaactttcc tgtactatag
     6661 gtgtatcatc taacaaactt gtagcaaaga ttgcatctga ttttaaaaaa ccagacggac
     6721 taacgatagt tacaccagac aaaatagaat cctttttgaa tccactacca atacgatcta
     6781 ttccaggaat tggaaaaaaa acagaagaga ggtttgcaga gatgaatctt gaaacaattt
     6841 ctcagctaaa aacacttgat gtgtttactc ttaacagcga gtttggcaga aagatcggag
     6901 catacatcta caatgctgca cgcggaatag ataatgaccc cgttgcacca agacaagaag
     6961 caacacaata cagcagaata actacactaa aacgagattc aaacgagtat gattttttgg
     7021 caaaagatct tgaaagatta tgtgaagaca tacatgccac catactaaaa gacaacattg
     7081 ttttcaaatc ggttggcata caatttgtac aagtggatct ttcaaacaaa actaggtcaa
     7141 aaactctgaa aaactctaca tcaaatcttg atgagttaaa aagagcagct ttacagttac
     7201 tccgtgaatc tttagaagat caaaaaatgc ttgtacgtag aattggtgta aaagtgtcag
     7261 agctctccga agtcacaggc caggacaaca ttaccagata tttttagaca gaaattccca
     7321 aacgagaagc caatcttttg tgctcgtctg gagttatcca ttccacatga aggtaatcaa
     7381 agatttcttg ttgattcata ccaatacgtt ttgcttctct tacagccact tcatatgcct
     7441 ctacctcagt aggcctatca acatacgaat aagatttgtc atagagatca agcccagatc
     7501 tttgttgttt tacatgcact agctcatgaa ctatatcaag atacaaaatc ttcgaatctg
     7561 attttttcag atgatcatat cctattacaa ttgttgcatc ttcattt
//
LOCUS       DATIDL010000128         2731 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrosopumilaceae archaeon isolate SMAG_U16342
            k141_2991069, whole genome shotgun sequence.
ACCESSION   DATIDL010000128 DATIDL010000000
VERSION     DATIDL010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935836
            Sequence Read Archive: SRR25158326
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosopumilaceae archaeon (soil metagenome)
  ORGANISM  Nitrosopumilaceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrosopumilales;
            Nitrosopumilaceae.
REFERENCE   1  (bases 1 to 2731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/04/2024 05:09:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,520
            CDSs (total)                 :: 1,474
            Genes (coding)               :: 1,474
            CDSs (with protein)          :: 1,474
            Genes (RNA)                  :: 46
            rRNAs                        :: 1 (5S)
            complete rRNAs               :: 1 (5S)
            tRNAs                        :: 43
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2731
                     /organism="Nitrosopumilaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2991069"
                     /isolate="SMAG_U16342"
                     /isolation_source="soil"
                     /db_xref="taxon:2202732"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1349
                     /locus_tag="VLB45_05625"
     CDS             <1..1349
                     /locus_tag="VLB45_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II/IV secretion system ATPase subunit"
                     /protein_id="HSD05216.1"
                     /translation="NQEEKRIFDMLHGLVEESLESPTTSKEDVDFESHLNRVIKDNEK
                     MFLGNSIISMEKVKYYLKRDISGYGLIDPLMHDVNIEDISCSGTDAPIYVWHRNYDSI
                     PTTIQFTSDKILNSFISRIVFRAGKHVSSAFPISDLALQGNHRLAVLYQKEITPKGTS
                     FTIRKFREDPYTIIDLISFGTIDLQIAAYLWMLVEAKMSVIIIGATGGGKTTILNSVA
                     GLVSPDHKIFSVEDVAEININHENWFTLVSRTGFGLSGEGEIGLYDLIKAGVRHRPDY
                     IIVGEIRGSEAYVMFQAMATGHGGLCTMHADSLESASKRLQQKPMDIPPAYISLMNCA
                     VVVKRVKDNATGQSSRRTISVSEILSADSSHQAFVWNPRTDYFDSNLQQSKLLQKIAE
                     YKGQTLNEVLVEHNKRVGILKWMLEHDIRDYRKVTEVIGKYYKDPEALIRKIESGA"
     gene            1352..>2731
                     /locus_tag="VLB45_05630"
     CDS             1352..>2731
                     /locus_tag="VLB45_05630"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HSD05217.1"
                     /translation="MLSLKNIDKLEYANKQKKKIERELPYFITIVTLLATSGLGPYFI
                     FQKIREFNLLPTIRAESEKILKRIDLLGVDPLTAMSQAKDKPSSKALGEFLGGYVSSM
                     QSGGNVINYLKSKMNSVFDRYAETEKQSISKVQALIEAYMTIQVVVLALYIMVTSIGP
                     TMNIGLSPSSGFKPDYILLILSPVVSAGFLFMSRGISHSKVKEIEIKKIVKFAAPSFG
                     IVTTLMITGIFSNIIGSGYILAVAFIASSLWPALRFKKIYATSLDAESATPQILRDIT
                     ESRKAGIGPEKCVIHACKRSDYKLFNPVTSAIATKLEWGIPLQNIFETLEKEIKNFQV
                     LINFRILIEVISSGGGSVQTLDALADTSEKFYNVDKNKRDMLKPYVMVGFMLIGITSF
                     TTLIVIDSFSNISQQSEIDEAKQAQLRKDSQSTLDLFSIAIVIQAWLAGLFLGKITTG
                     SFSGGFMYSI"
BASE COUNT          975 a          465 c          524 g          767 t
ORIGIN      
        1 taaatcaaga agaaaaaaga atttttgaca tgttgcatgg ccttgtagaa gagagcctag
       61 agtcgccaac tacctctaaa gaagatgttg attttgaatc tcatctgaac agagtaatca
      121 aagataatga gaaaatgttt cttggcaatt ctatcataag tatggaaaag gtcaaatatt
      181 atttaaaaag agacatcagc ggatacggat taatcgatcc actaatgcat gacgtcaata
      241 ttgaagatat tagttgcagt ggaacggatg caccaatcta tgtctggcat agaaactatg
      301 acagcattcc aacaaccatt caatttactt cagataaaat attgaactca tttatttcaa
      361 gaattgtttt cagagctggc aaacatgtca gtagtgcatt tcctatttca gatcttgcac
      421 tacaaggaaa tcatagactt gcagtgttat atcaaaaaga aatcactccg aaaggaacaa
      481 gctttacaat aagaaagttc agagaagatc cttatacaat aatagatctt atttcatttg
      541 gaacaattga cctacaaatt gctgcatatc tttggatgct agttgaagca aagatgtcag
      601 taattatcat aggtgcaacc ggcggtggta aaaccactat tcttaattca gtagctggac
      661 ttgtaagtcc tgatcataaa attttctctg tagaagacgt tgctgaaata aacatcaatc
      721 atgagaactg gtttactcta gtatcaagaa ctggttttgg ccttagtgga gagggagaaa
      781 ttgggttata cgatttgatc aaagcaggag tcagacacag accagactat atcatagttg
      841 gagaaatcag gggttctgaa gcatatgtga tgtttcaagc catggctaca ggacacggcg
      901 gattatgtac aatgcacgca gacagtttag aatctgcaag caaaagatta caacaaaaac
      961 caatggacat tccgcctgca tatatttcac tgatgaactg tgcagtagtg gttaaacgag
     1021 taaaagataa tgcaaccgga caaagtagca gaagaacaat atccgtatca gagattctta
     1081 gtgcagattc atctcatcaa gcatttgtct ggaatccaag aactgactat tttgatagca
     1141 atttacagca aagtaagcta ttgcagaaaa tagctgaata taaaggacaa acgctaaacg
     1201 aagtcttggt ggaacacaac aaaagagttg gaattttaaa atggatgctt gaacatgaca
     1261 ttcgcgatta taggaaagtg actgaggtaa taggaaaata ctacaaagat cctgaagcgc
     1321 ttatccgaaa gatagaatca ggagcctagg catgttgtca cttaagaaca tagacaagtt
     1381 ggagtatgca aacaaacaaa aaaagaaaat cgaacgtgaa cttccatatt ttattacaat
     1441 agtcacactg cttgcaacaa gcggtcttgg tccttatttt atttttcaaa aaattagaga
     1501 gtttaatcta ctgcctacaa ttagggcaga gtcagaaaag attctaaaaa gaattgactt
     1561 actaggtgtt gatcctctca ctgcaatgag tcaagcaaaa gacaaacctt catcaaaagc
     1621 attaggagaa tttcttggcg gctacgtttc ttcaatgcag agtggtggca atgttatcaa
     1681 ttatctaaaa agtaaaatga atagtgtatt tgacagatat gccgaaaccg aaaaacaatc
     1741 catatcaaaa gttcaggcgt tgattgaggc atacatgaca atacaggttg tggttcttgc
     1801 cctatacatc atggtcacat caatcggccc taccatgaat ataggattaa gtccatcatc
     1861 tggattcaaa cccgactata tcttactgat cttgtctccg gttgtatcgg ctggatttct
     1921 attcatgagt cgcggcatat cacattccaa agttaaggag atcgaaataa aaaaaattgt
     1981 aaaatttgca gctccaagtt ttggaattgt aaccactctc atgattacag gaatattttc
     2041 aaatattatt ggaagtggtt atattttggc ggtggcattt attgcatcat cattatggcc
     2101 tgcattaaga tttaagaaaa tttatgccac aagcttagat gcggaatctg caacaccgca
     2161 aatcttaaga gatattacag aatcaagaaa ggctggaatt ggaccagaaa aatgtgtaat
     2221 acacgcatgc aaaagatccg attacaaact tttcaatcca gttacaagtg ccattgcaac
     2281 aaaattagaa tggggtatac cattacagaa tatttttgaa acacttgaaa aagaaatcaa
     2341 gaattttcaa gtgttaatca attttagaat tctaattgag gtaatttcat ctggtggtgg
     2401 cagtgtacaa actcttgacg ctttagctga cactagtgaa aaattctaca atgttgacaa
     2461 aaataagcgt gacatgctaa agccatacgt catggtaggt ttcatgttga taggaatcac
     2521 cagctttacc accctgattg taatagattc gtttagcaac ataagccaac aatcagaaat
     2581 agatgaggca aaacaagcac aacttagaaa agactcacaa tctaccttgg atctcttttc
     2641 aatagcaatt gtaattcaag catggcttgc aggattattt cttggcaaaa taactacagg
     2701 ttcattctct ggaggattca tgtattctat a
//
LOCUS       DATIDL010000129         4861 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrosopumilaceae archaeon isolate SMAG_U16342
            k141_969672, whole genome shotgun sequence.
ACCESSION   DATIDL010000129 DATIDL010000000
VERSION     DATIDL010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935836
            Sequence Read Archive: SRR25158326
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosopumilaceae archaeon (soil metagenome)
  ORGANISM  Nitrosopumilaceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrosopumilales;
            Nitrosopumilaceae.
REFERENCE   1  (bases 1 to 4861)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4861)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/04/2024 05:09:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,520
            CDSs (total)                 :: 1,474
            Genes (coding)               :: 1,474
            CDSs (with protein)          :: 1,474
            Genes (RNA)                  :: 46
            rRNAs                        :: 1 (5S)
            complete rRNAs               :: 1 (5S)
            tRNAs                        :: 43
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4861
                     /organism="Nitrosopumilaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_969672"
                     /isolate="SMAG_U16342"
                     /isolation_source="soil"
                     /db_xref="taxon:2202732"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..427
                     /locus_tag="VLB45_05635"
     CDS             <1..427
                     /locus_tag="VLB45_05635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05218.1"
                     /translation="AFGAISDKTGLKKDFVIETGGYDFEVETVANFEINDVQFSRDDK
                     KITFDITSSLENNFAEVQIPINLIDGDLTFFVNGQEIFPDIRKNDKISFIVLEFEGVG
                     HNVLEIIGTTYLPEFSGIAILVMSLAFTAVMLSRKFQKI"
     gene            complement(424..1140)
                     /locus_tag="VLB45_05640"
     CDS             complement(424..1140)
                     /locus_tag="VLB45_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05219.1"
                     /translation="MPFIKIKLRLDEWGTDLYELRHKRNWVDLSIFAGAAAFAIIVFL
                     TFGPDIERTTRESPIIMNIMFGPAFAIGLLYGIKLTDGVMRPSETRSPFKRAIVKTFL
                     FLFMMGAIFSSVTFALHGGMDSPEKSIFEDGLIPWMSEFVESNGGLTFLIVSSITIMA
                     AATRRIVGMEGFLSKVFTFVGTFIFFSMISLSLSHTDPTHSQVYLYAFYQAGIIGGIF
                     YQMNKLTSRANLWEDFANGY"
     gene            1220..1441
                     /locus_tag="VLB45_05645"
     CDS             1220..1441
                     /locus_tag="VLB45_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008298159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05220.1"
                     /translation="MKHGKLLIISGMIIIASGTVFYFQGQSVIGPESSFMYSNPEWIT
                     NGQWIVIIGIIVLAAGIVTSKKSNLRLPK"
     gene            complement(1419..1649)
                     /locus_tag="VLB45_05650"
     CDS             complement(1419..1649)
                     /locus_tag="VLB45_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HSD05221.1"
                     /translation="MEKAYILIGCELGSESDLVTKILELDRVKDARVTYGDYDIVVEA
                     ETETEAQMDNLITKKIRKLDKIRTTITLGVVS"
     gene            1842..2231
                     /locus_tag="VLB45_05655"
     CDS             1842..2231
                     /locus_tag="VLB45_05655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05222.1"
                     /translation="MNLKIASIFSVLVLLLGITSVTHSAFAVEDTQKSLLKKDLSSIA
                     YDYKMSVLKAQTDLIKAVQKANADAKASIQKGIPMEKINAASKSYIDKARTDFKLAIA
                     KAKADAKSTLLQIKSAVDQKNLFNSKS"
     gene            complement(2220..3383)
                     /gene="truD"
                     /locus_tag="VLB45_05660"
     CDS             complement(2220..3383)
                     /gene="truD"
                     /locus_tag="VLB45_05660"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HSD05223.1"
                     /translation="MYSTDFEGAGGNIKSTPDDFFVSEVLDAKTLSKISRQNGYAVYR
                     LKKRGIDTNHALAEIFHVHGLRLKALGLKDANATTEQFVCNTSISKAPEKFSANRYSI
                     ELLGFVPKPLTKKDMIGNQFKIKISNTSFSKILNFKSYDRILNFYGYQRFGSKRPVSH
                     LIGKAILQNDFPKAVELLLSFSSDYDLPENTKIRKTLADKSNYSKMLDEIPPQMDLER
                     IVLGEMTEHNDPLKAIRAIPLSIRRLFVQAFQSYIFNCSLSLAFENSEELFSPKEGDV
                     CYDMKNMLGRYENDPEQRLAIPIVGYSYSKKNRFDYYISKILEKEQVHAKDFFSKTMQ
                     EISDEGGFRQAAIMCSDISVSEPTISFTLSRGSYATILLREIIKPEDPIKSGF"
     gene            complement(3423..>4861)
                     /locus_tag="VLB45_05665"
     CDS             complement(3423..>4861)
                     /locus_tag="VLB45_05665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="lyase"
                     /protein_id="HSD05224.1"
                     /translation="GTVTKTGTPADNYKDAERETFCGTGSVKSNRYITEFKIPTVCTQ
                     PLAITTDSSGNVWFTESNTGNLAKFDPFSRTFTEFENTEWPEGDQSMMWGIDHSQDEN
                     IWFTDERHDIIWKFSILEKNFTRYKYPASDSEATFPQKLIVDGQQVIVNDFSGNKITF
                     FDTSQSGQEIKYSKIDSPTADQDSVTGAFTIDSNGKLWYTVWKFQQGGNLLRYDSQDN
                     NGTAFKLPLGIQAPNGISSSPEGKIWITDAASSFFFSFDPQSQQFTKFITPPPSEVTY
                     GNSSGLIKTPISRPYWNDFDNKGRLWFNEQTGNSIAVFDPKKESLVEYGVPSGNPNWS
                     DCGNLEDCGVAQVLDFTIRDDKIWFTEWVENNIGVLDSSVPLPFDIDVDENNLILNRD
                     SNVTLTLTISPTEHLSEPVDIVTANTAGLRDITINHEQKTTVGDQPQTVTFDVYVDKF
                     ALSGTYKILLGARYHEVTVSEYVTVTVK"
BASE COUNT         1485 a          907 c          842 g         1627 t
ORIGIN      
        1 cgcatttggt gcaatttcag ataaaacagg tctgaaaaag gattttgtta ttgaaactgg
       61 tgggtatgat tttgaagtgg aaacagtagc aaattttgaa attaatgatg tacaatttag
      121 cagggatgac aaaaaaataa cgtttgatat aaccagctca ctcgagaata actttgctga
      181 ggtacaaatt ccaataaatc tcatagatgg cgatcttacc ttctttgtaa atggtcaaga
      241 aatatttcca gatattagaa aaaatgataa aatatcattt attgtacttg aatttgaagg
      301 agtaggacat aatgtacttg aaataattgg aaccacttat cttccagaat tttcaggtat
      361 tgctatactt gttatgagtc tagcattcac agctgtaatg ttatcaagaa aatttcaaaa
      421 gatctagtaa ccatttgcaa aatcttccca caggtttgca cgtgatgtaa gcttattcat
      481 ttgatagaat atgccaccta ttatgccagc ctgatagaat gcataaagat acacttgaga
      541 gtgtgttgga tctgtatgcg aaaggcttag agatatcatt gaaaagaaaa tgaaagtacc
      601 tacaaaagta aagactttac taaggaatcc ctccatgcca acaattcgtc ttgtggcagc
      661 tgccattatc gttatgctag aaacaataag aaaggttaat ccaccattag attctacaaa
      721 ctcactcatc catggtatca ggccatcttc aaaaattgat ttttctggcg aatccatgcc
      781 accgtgtaag gcaaaagtga cagagctgaa gattgcaccc atcatgaata aaaacaaaaa
      841 tgtctttaca attgctcgct tgaatgggct ccttgtttct gaaggcctca tcacaccatc
      901 tgttagcttt atgccgtaaa gcagacctat ggcaaaagca ggaccaaaca ttatgttcat
      961 tatgattggt gattctctgg ttgttctttc tatatctgga ccaaatgtca aaaatacaat
     1021 tattgcaaaa gctgctgctc cagcaaatat gctgaggtcc acccaatttc gtttatgcct
     1081 aagttcgtat aggtctgttc cccactcatc caatctgagc ttgattttga taaaaggcat
     1141 taaagaaatg actaaaaaat gatttgctta atctatcaat tctctttacc ttttatggta
     1201 catttgagat tgattatgcg tgaaacatgg gaaactgctg ataatctcag gtatgattat
     1261 tatcgcatcg ggaacagtat tctattttca aggtcaatca gtaattggac cagagtcttc
     1321 tttcatgtac tccaaccctg agtggataac aaatggtcag tggatcgtaa ttattggaat
     1381 aattgtcttg gcagcaggaa ttgtaacatc aaaaaaatct aacttacgac tcccaaagtg
     1441 attgtggttc ttatcttgtc tagtttgcga attttttttg ttattaaatt gtccatctga
     1501 gcttctgtct ctgtttctgc ttctacaaca atatcataat caccatacgt cactcgagca
     1561 tctttgactc tgtccaattc taaaatcttg gtgactagat cactttcaga acccagctca
     1621 catccaatca aaatgtatgc tttttccata atgtactaat ataatacgat cttaaatctt
     1681 atcacattat cttgagtgtc tctagtacgt ttgttatgat gtaacaggcc ctaaccagat
     1741 gattgttcgt gtctgttatg tatgatttat tttcaaacat aaaacttcac gcagaaatcg
     1801 tacgtaatac tttttaattg gtttgaatga gcttttttga aatgaatttg aagatagctt
     1861 caatctttag tgtactggtt ttgctgttgg gtattacaag tgttacacat tctgcctttg
     1921 ccgtggaaga tacgcaaaaa tcactcttaa agaaggatct aagttcgatt gcatatgact
     1981 acaaaatgtc tgtactcaaa gcgcaaaccg atctgataaa agccgtacag aaagcaaacg
     2041 cagacgcaaa agcttcaata caaaaaggaa ttccaatgga aaaaatcaac gctgcatcaa
     2101 aatcatatat tgataaagct agaactgact tcaaattggc aatagcaaaa gcaaaggctg
     2161 acgcaaaatc aacattattg caaataaaat ctgctgtaga tcaaaagaat ctatttaatt
     2221 caaaatcctg acttgatagg atcctctggc ttgattatct cacgcaaaag aattgtggca
     2281 taagatcctc ttgagagtgt aaaactaatt gtaggttctg atacggaaat gtcactacac
     2341 attattgccg cttgtctgaa tccaccttca tcacttattt cctgcatggt ttttgaaaag
     2401 aaatcctttg catgaacctg ttctttttca aggatctttg aaatatagta atcaaatctg
     2461 tttttcttag aataagaata tcctactatc ggtatggcaa gtctctgttc tggatcattt
     2521 tcatatctgc caagcatatt tttcatgtca taacaaacgt caccttcttt tggagaaaat
     2581 aattcttcac tgttttcaaa agcaagactc aatgaacaat tgaaaatata cgactggaag
     2641 gcttgaacaa acagtcttct aatggaaagt ggaattgctc ttattgcttt gagaggatcg
     2701 ttatgctctg tcatctcgcc aagcacgatc ctttccaaat ccatctgagg cggaatttca
     2761 tctagcattt ttgaataatt tgacttgtct gcaagtgtct ttctaatctt agtgttttct
     2821 ggcaaatcat aatctgagct aaaggaaaga agaagttcta cagcttttgg aaaatcattt
     2881 tgtagtattg cctttcctat caaatgtgat actggacgtt tacttccaaa cctttggtat
     2941 ccgtagaaat tcaaaatcct gtcatagctt ttgaaattta aaatctttga aaatgaggtg
     3001 tttgatatct ttattttaaa ctgatttcct atcatgtctt tttttgtaag cggctttggc
     3061 acaaatccca acaactcaat actgtatcta ttggcagaga atttttccgg tgcttttgat
     3121 atgcttgtat tgcatacaaa ctgttctgtt gtagcatttg cgtcctttag ccccaaggcc
     3181 ttcaatctca gaccatgcac atggaaaatt tctgccaatg catggtttgt atctattcca
     3241 cgtttcttga gtctgtatac tgcatatcca ttctgtcttg aaatttttga taatgtcttt
     3301 gcatccaaga cttcagatac aaaaaaatcg tctggcgtag atttgatatt tcctccagca
     3361 ccttcaaaat ctgtactata cacagaaata ccaatatcaa aatctaattt tggaatcact
     3421 tcttatttta ctgtgactgt aacatattct gaaaccgtga cttcgtggta tcttgctccc
     3481 aaaagaattt tgtatgttcc agatagtgca aatttatcaa catacacatc aaaggtaaca
     3541 gtttgaggtt gatctccaac agttgttttt tgttcatgat ttatcgtgat gtcccttagc
     3601 cctgcagtat ttgcagtaac gatatcaact ggttcactga gatgttcagt tggagaaatt
     3661 gttaatgtca gagtaacatt gctgtctcta tttagaatca gattattttc atctacatct
     3721 atatcaaacg gtaatggtac actggaatca agaactccta tgttgttttc aacccattct
     3781 gtaaaccata tcttgtcatc tcttattgta aagtcaagca cttgagctac tccacaatct
     3841 tcaagatttc cacaatctga ccagtttgga ttcccagatg gtacgccgta ttcaactaga
     3901 gactcttttt tgggatcgaa aactgcaata ctgtttcctg tctgttcatt aaaccataat
     3961 ctgcctttgt tatcaaaatc gttccaatat ggccttgata ttggggtctt gattaatcca
     4021 gaagaatttc catatgttac ctcacttggt ggaggagtaa tgaattttgt aaattgttgt
     4081 gattgtggat caaaactaaa aaagaaactg cttgcagcat ctgtaatcca aatcttgcct
     4141 tctggactcg atgatatgcc gtttggagcc tgtattccta gaggaagctt aaacgcggtt
     4201 ccgttattgt cttgtgaatc atagcgtaaa agatttccac cctgttggaa tttccaaact
     4261 gtataccaga gtttgccatt ggaatcgata gtaaatgctc ctgtaacaga atcctgatca
     4321 gcggttggag aatcaatctt tgaatatttt atctcctgcc cagattgact agtatcaaaa
     4381 aatgttattt tgttgccaga aaagtcattt actataactt gttgcccatc aactatgagt
     4441 ttttgtggaa aagttgcttc tgaatcagaa gctggatatt tgtaacgtgt aaaatttttc
     4501 tctaaaatag aaaacttcca gataatgtca tgacgttcat ctgtaaacca gatgttttca
     4561 tcttgggaat gatctattcc ccacatcatg gattggtcgc cctctggcca ctctgtattc
     4621 tcgaattctg tgaatgttct cgaaaaggga tcaaactttg caagatttcc tgtatttgat
     4681 tctgtgaacc acacatttcc tgatgaatca gttgttatgg caagtggctg tgtacacaca
     4741 gttggaattt taaactcagt tatgtatcta ttagatttta cactgccagt accgcaaaaa
     4801 gtttctctct cggcatcctt gtaattgtca gcaggcgttc cagtttttgt aactgtacca
     4861 t
//
LOCUS       DATIDL010000130         3961 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrosopumilaceae archaeon isolate SMAG_U16342
            k141_2182484, whole genome shotgun sequence.
ACCESSION   DATIDL010000130 DATIDL010000000
VERSION     DATIDL010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935836
            Sequence Read Archive: SRR25158326
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosopumilaceae archaeon (soil metagenome)
  ORGANISM  Nitrosopumilaceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrosopumilales;
            Nitrosopumilaceae.
REFERENCE   1  (bases 1 to 3961)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3961)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/04/2024 05:09:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,520
            CDSs (total)                 :: 1,474
            Genes (coding)               :: 1,474
            CDSs (with protein)          :: 1,474
            Genes (RNA)                  :: 46
            rRNAs                        :: 1 (5S)
            complete rRNAs               :: 1 (5S)
            tRNAs                        :: 43
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3961
                     /organism="Nitrosopumilaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2182484"
                     /isolate="SMAG_U16342"
                     /isolation_source="soil"
                     /db_xref="taxon:2202732"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..777)
                     /locus_tag="VLB45_05670"
     CDS             complement(<1..777)
                     /locus_tag="VLB45_05670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05225.1"
                     /translation="MSQQTRHKKIALTAAIAGAVAVGLFVAYFVPLQTELTTIPPTQE
                     EQERSEALGVAQRFIVTSPTFAFDGMIDTLDTVYVGSTKSIPPQYIIKISFDSAHGGF
                     GDREGQILTQVITPHTMEIVVSEGIVISAVTDETWDELNNQYVLKKPESKLQSHDEPI
                     VPFDGVVTDYSSLVDAIKSRGILVTPIDEIPAEISSFSVPTRVISVGGADVQVFEFAS
                     EQDVIAATQTVSEDGTEIGTSIIRWISPPHFYAQGNLIVLY"
     gene            complement(970..3069)
                     /locus_tag="VLB45_05675"
     CDS             complement(970..3069)
                     /locus_tag="VLB45_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008298834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HSD05226.1"
                     /translation="MAKDPVCGMIVDEKTALNSEIGARTFYFCSPTCLNTFVNPEKEL
                     GKLKKRMYVAVSGALILAILRGGLYLALAFGAIAVTWVPFPQIPFLSWGMLLFIIVTP
                     VQFIGGWTFYVGAYQAIKRRTANMDLLISIGTLVAYFYSVTVLFFPGALPVKERDVYF
                     EVSAVIIAFVLLGKYMEEAIKKRSSAAVRKLLDLRPATARIIKDGKEIEIPANQIQVG
                     DILIIRPGEKIPTDGVVIEGISAVDEKMITGESIPVDKTVGSEVIGATLNKNGLLRIK
                     ATKVGKETALSQIVHIVEEAQASTGHIQRLADSISAKFVPVVVSVAVGSFLVWYFVFG
                     NFITGLLSFVAVLIIACPCAMGIATPAALMVGVGKGAEAGILIRGAEYLERSQKIKTI
                     VFDKTGTLTKGEPSVTDLLSLKNYTDIQLLEFAGSVESGSEHPLAQAIIKEAKTRSIS
                     LQNPTQFESVSGMGVKANISGKQILFGNRKLMTTFGIAIDVEQQMAGLESQGKTVMIV
                     AVEKEIAGLIAVADTLKESSSAAISSLKKLGIETVMLTGDNEKTARTVAQNVGIDKVI
                     ANVLPAEKANVIKKLQSEGKVVAMVGDGINDAPALAQADIGIAIGSGSDIAKETGGII
                     LIKDDIMDVARAVKLSRATMRKIKENLFWALAYNTGAIPIAAIGLLSPIIAAAAMALS
                     SISVIANSSLLKRLKIT"
     gene            3188..3373
                     /locus_tag="VLB45_05680"
     CDS             3188..3373
                     /locus_tag="VLB45_05680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05227.1"
                     /translation="MFEKHCQICGIDIKKETAVKTFGKFFCDESHAKEYLKKKEEYEK
                     IMEAEERKHLRRHGGCC"
     gene            complement(3449..>3961)
                     /locus_tag="VLB45_05685"
     CDS             complement(3449..>3961)
                     /locus_tag="VLB45_05685"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HSD05228.1"
                     /translation="NPQFGNWMMGPMMNNPDTMNSMMNQMMQNPQFMNKWMGQMMTNQ
                     NFQQQYMGPYMMMRDPQFMQNMRNQYYQADIGSLAVETDKVSILADTWQYQSTKSYYP
                     EIIKVSPNTTVTWTNEDQIIHTVTDLGNKFDSGFIQPQESWQYTFDKQGNYLYFCSIH
                     PWMKGTVIVS"
BASE COUNT         1081 a          801 c          743 g         1336 t
ORIGIN      
        1 atataggacg attagattgc cttgtgcgta aaagtgcggt gggcttatcc agcgtatgat
       61 gcttgttccg atttctgttc cgtcttctga aacagtctgt gttgcagcaa ttacatcttg
      121 ctcacttgca aactcaaaga cttggacgtc tgctccgcca acagagatca ctctggtcgg
      181 tacggaaaat gacgagattt cagctggaat ttcatctatt ggtgttacca ggatgccgcg
      241 tgattttatt gcatcaacta gtgatgaata gtcagtaacc actccatcaa atggtacgat
      301 tggctcgtca tgtgattgaa gttttgattc tggttttttt agtacgtatt ggttgtttag
      361 ctcatcccat gtttcatcag ttactgcaga aattacaatt ccttcagata caacaatctc
      421 cattgtgtgt ggagttatga cctgagtaag aatttgtcct tccctgtctc caaatccgcc
      481 gtgtgcagag tcaaagctta tttttatgat gtactgcggt ggaattgatt ttgtggaacc
      541 gacatataca gtgtctagtg tgtctatcat tccatcaaat gcaaatgtcg gactggttac
      601 gatgaacctt tgtgctacac ccaacgcttc gcttctttct tgctcttctt gtgttggcgg
      661 tattgttgta agctcggttt gcagaggtac aaaatacgcc acaaaaagac ctactgcaac
      721 agcgccagct attgcagcag tcaaggcaat ttttttgtgt cgtgtttgtt gcgacatgtt
      781 ttatcaaact agtatggttt gaattgatta taacctttga ttaaatttca atttaacata
      841 attaattgat aaaaaattat gtttttgaaa tgtgttttga atatttttta tattgtatta
      901 gttttggtga aaatttcgtt tttctgtagt cagacaaatg ataatgtcag attgtcttag
      961 tgtagtaatc taggtaattt tcaatctctt aagaagcgag gaatttgcta tcacagatat
     1021 tgagctgagg gccatcgctg cagctgcaat tatcgggctt aacaatccta ttgcagctat
     1081 tggaattgct ccagtattgt aagccagtgc ccagaataga ttttctttga tctttctcat
     1141 cgtggctcta ctaagcttta cggctcttgc cacatccata atgtcatctt ttatcagaat
     1201 tattcctcca gtctcttttg caatgtcaga accactccct atggcaattc caatgtctgc
     1261 ttgtgcaagt gcaggtgcat cgtttatccc atctccaacc attgcaacaa cctttccttc
     1321 tgattgtaat ttttttatga catttgcctt ttctgcagga agtacgtttg ctatcacttt
     1381 gtcaattcca acattttgtg caactgttct ggctgtcttt tcattgtcac ctgttagcat
     1441 caccgtttca atgccaagtt ttttcagtga gcttattgcg gcagatgatg attcctttag
     1501 agtgtctgca acagcaatca gtccagctat ttctttttct actgcaacta tcatgacagt
     1561 ttttccctgt gactctagac ctgccatttg ctgctcaaca tctattgcaa tgccaaacgt
     1621 tgtcatcagt tttctgtttc caaaaagaat ttgtttacca gatatgttgg ctttgacacc
     1681 cattcctgaa acagattcaa actgtgtagg attttgcaaa gagatgctgc gtgttttggc
     1741 ttctttgatg attgcctgtg caagtggatg ttcagagccg ctttccacac tgcctgcaaa
     1801 ttctagcaac tggatgtctg tgtagttttt caaagaaaga agatcagtga ctgaaggttc
     1861 accttttgtc aaagttcctg tcttgtcaaa gacaatggtc tttatttttt gagaacgctc
     1921 aagatattca gctcctctga tcagaatgcc tgcttctgca cccttcccta ctcctaccat
     1981 gagagctgct ggtgtggcta ttcccatggc acatggacag gcaattatga gtactgcaac
     2041 aaaagaaagt agaccagtaa tgaaatttcc aaaaacaaaa taccacacca aaaaagatcc
     2101 tactgccact gaaacaacga ctggaacaaa ttttgcagaa atagaatccg ctagtctctg
     2161 aatgtgtcct gttgaagctt gcgcctcttc aactatatgc actatttgtg aaagtgcagt
     2221 ttcttttcct acctttgtag ctttgatcct aagtaatcca tttttgttta gagttgctcc
     2281 tatcacctcg cttcctactg ttttatctac agggatgctt tcacctgtga tcattttttc
     2341 atccactgca gatattcctt caattacaac accgtctgtt ggaatctttt ctcctggcct
     2401 tatgatcaaa atgtcgccaa cttgaatttg attggctgga atctcaattt cctttccatc
     2461 ttttattatt cttgcagtag caggtctaag atctaaaagt tttcttactg cagctgagct
     2521 tctttttttg attgcttctt ccatgtattt tcctaacagt acaaaagcaa tgataacagc
     2581 agagacttca aagtaaacat ctctttcttt gacaggcaaa gcaccaggaa aaaacaagac
     2641 tgttacactg taaaaatatg caacaagcgt accgattgat atcaaaagat ccatgtttgc
     2701 agttcttcgc ttgattgcct gatatgcacc aacatagaaa gtccacccgc cgatgaactg
     2761 gactggtgtc acaatgataa acaataacat gccccaagag agaaacggta tttgtggaaa
     2821 aggcacccaa gtaaccgcta ttgctccaaa tgccagtgca agataaagac caccacgcaa
     2881 tattgcaaga atcagtgcac cagaaactgc aacgtacatt cttttcttga gtttcccaag
     2941 ttctttttcg ggatttacaa atgtgttcaa acaggtagga ctgcaaaaat aaaacgtcct
     3001 tgcaccaatt tctgaattaa gtgcggtttt ttcatccact atcattccgc atactgggtc
     3061 ttttgccatc ttgtcacaaa ttggttgatt tcttcgtttt tagttgaacg cggtgattat
     3121 catgattgat tctcatggat aaaaagcaaa actagtgatt atatcttaca atcctagtgt
     3181 gcaatatatg tttgaaaaac attgccagat ctgtggcatt gacataaaga aagaaacagc
     3241 tgtcaagaca tttggaaaat tcttttgtga tgaatcacat gcaaaagaat atctaaagaa
     3301 aaaagaagaa tatgaaaaaa taatggaagc agaagaaaga aaacatctgc gtagacatgg
     3361 aggatgttgt tagattatat gttttccaaa gtgtaaatta tacaaactag accaatatca
     3421 aaaaatattc atattaaatt tgaaattttt atgacactat gacagtgcct ttcatccacg
     3481 gatgtatgct gcaaaagtaa agatagttgc cttgtttgtc aaaagtgtac tgccatgatt
     3541 cttgcggctg gataaatcca gaatcaaact tgtttccaag atcagtcacc gtgtgtatta
     3601 tttggtcctc gtttgtccac gttactgtcg tgtttggtga tactttgatt atttctggat
     3661 aatatgattt tgttgactgg tactgccaag tgtctgccaa aatggagacc ttgtctgttt
     3721 caactgccaa gcttccaatg tctgcctggt aatattggtt tctcatgttt tgcataaact
     3781 gcgggtctcg catcatcatg tatggtccca tgtattgctg ctgaaagttt tggtttgtca
     3841 tcatctggcc catccatttg ttcataaatt gcggattttg catcatttgg ttcatcatcg
     3901 aattcatcgt atcaggatta ttcatcattg gtcccatcat ccagttgcca aactgtggat
     3961 t
//
LOCUS       DATIDL010000131         3346 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrosopumilaceae archaeon isolate SMAG_U16342
            k141_972510, whole genome shotgun sequence.
ACCESSION   DATIDL010000131 DATIDL010000000
VERSION     DATIDL010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935836
            Sequence Read Archive: SRR25158326
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosopumilaceae archaeon (soil metagenome)
  ORGANISM  Nitrosopumilaceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrosopumilales;
            Nitrosopumilaceae.
REFERENCE   1  (bases 1 to 3346)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3346)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/04/2024 05:09:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,520
            CDSs (total)                 :: 1,474
            Genes (coding)               :: 1,474
            CDSs (with protein)          :: 1,474
            Genes (RNA)                  :: 46
            rRNAs                        :: 1 (5S)
            complete rRNAs               :: 1 (5S)
            tRNAs                        :: 43
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3346
                     /organism="Nitrosopumilaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_972510"
                     /isolate="SMAG_U16342"
                     /isolation_source="soil"
                     /db_xref="taxon:2202732"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(14..745)
                     /locus_tag="VLB45_05690"
     CDS             complement(14..745)
                     /locus_tag="VLB45_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HSD05229.1"
                     /translation="MEIIKIRTGIPGFDSLISGGFREGRTIILSGPPGGGKTTFGMQF
                     LHSGAKDFDEPGVFVTLSESPNEIKNDFKTYGWDIQKLVDEGKLLIIDARPFKMEDGF
                     VALDDSLYRGETLPFMHLTQLILSSIKRIGAKRLVIDSLTVLGLQYANKFYARQGLQG
                     MIQALEDQRCTSIMISENMDVNKTPTEWYVASGVVLLSHIQRENSIERTVQVIKMRGM
                     RHSEQIHPVKLGESGILIPHPRLVS"
     gene            916..2064
                     /locus_tag="VLB45_05695"
     CDS             916..2064
                     /locus_tag="VLB45_05695"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007550520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HSD05230.1"
                     /translation="MTKILGIIGGGQLGMMLTEAAKKMPDQISDVIVLDPTRDCPATK
                     VGAKQIVADFKDKNAIVELSLQSDIITYEIESGNSEVLESLNGNVIVNPSPTTLRTIQ
                     DKCVQKKFLHEKGLPVADFVEIESIYALKEKIAAFGYPAMLKIRRDAYDGRGNFRIEK
                     PEHITQAYEHFGGKPIMLEKVIDFQMEISVIAARNIKGEVATYQPVENIHEENILKMT
                     IAPARISKETSQEAEHIAKKTMEVLQGAGVFGIEMFVTRDDKVLINEIAPRVHNSGHH
                     TLQSSATSQFEQHLRAILGLELGKTDLLHYAVMCNILGPKDFHGKYQPISLEEKDGMY
                     LKMYQKEEAKPQRKLGHFNVIDLEDTKDIDALIRKAEQIKSLIKFKSV"
     gene            2069..2644
                     /gene="purE"
                     /locus_tag="VLB45_05700"
     CDS             2069..2644
                     /gene="purE"
                     /locus_tag="VLB45_05700"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HSD05231.1"
                     /translation="MFYSKKPLVGIIMGSSSDSKIMHGAAMILDEFGVKHEDQIVSAH
                     RTPSRLADYAKHAEKSGFKVIIAGAGGSAHLPGMIASHTVIPVIGVPIMVYNDKSGSS
                     DRFKFSAFGGLDSLLSISEMPTGSPVVTVGVNKATNAGLYALKILANEFEDLQKKLKK
                     HKEKQHHSVIRESDEMKKLGLSKFAKKKYKM"
     gene            2659..3198
                     /locus_tag="VLB45_05705"
     CDS             2659..3198
                     /locus_tag="VLB45_05705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05232.1"
                     /translation="MDIKEILYNSIERHKKYIISEITQGKSNETIDSICKECMPLLEK
                     LEGDKSEIMISFAEGLVHYLLTTSLIPSQRKITVDDTYVDISIPDIKTLKSNPQDALL
                     IIFPKTKNIQEIKNRASELKKIQPIQENTWFVLENDVDIKAIVYSIYNWKIPFSQILN
                     DIIGFLTNKKQSKLKLFRI"
BASE COUNT         1232 a          578 c          588 g          948 t
ORIGIN      
        1 taacagcata attttacgaa accaatcttg gatgtggaat caatattcct gattctccta
       61 atttgacagg atgaatttgt tcactatgtc gcataccgcg catcttaatt acctgtactg
      121 tccgttcaat agaattttcc ctttgtatgt ggcttaaaag aacaaccccc gaagcaacgt
      181 accattctgt aggagtcttg ttgacatcca tattctcaga tatcattata gaggtacagc
      241 gctggtcttc taaagcctga atcattcctt gtaggccctg tcgtgcatag aatttgtttg
      301 cgtattgcaa tcctaataca gtaagtgagt caataaccaa acgttttgca cctatccttt
      361 tgatgctgct taaaatcaat tgagtcaaat gcataaacgg caaagtttcg cctcgatata
      421 atgaatcatc cagggcaaca aacccatctt ccattttaaa tggtcttgca tcaattatca
      481 acaattttcc ttcatcaaca agtttttgaa tatcccatcc ataagtttta aaatcgtttt
      541 taatttcatt tggactttca gaaagagtta caaaaactcc aggttcatca aaatcttttg
      601 caccagaatg tagaaactgc attccaaaag ttgtcttacc gccacctggt ggacctgaca
      661 agattattgt tctaccctct ctgaatcctc cagatattaa agaatcaaat ccaggaattc
      721 cagtcctgat ttttattatt tccacaagat aaaatctcac aaaagtgtat ttaaagagta
      781 gtaatatttt tgacataact gtttccattt gtggaaatac tttctagatg aaattttgta
      841 taaatagaaa cttacagatc taaatgagat catttatcgc tagctaattt taaaagacag
      901 ttgggaaaat caacaatgac aaaaattcta ggcataatag gcggaggtca actcggcatg
      961 atgctaacag aagcagcaaa aaagatgccc gatcagatct cagatgtcat cgtattagat
     1021 ccaacaaggg attgtcctgc aacaaaagtc ggtgcaaagc aaattgttgc cgattttaag
     1081 gacaaaaatg caattgtaga gctgtcttta caatctgata ttataacata tgaaatagag
     1141 tctggaaaca gcgaggtttt agagtcacta aacggcaatg taattgtaaa tccgtctcct
     1201 acaacactac gaacaataca agacaaatgt gtgcagaaga aatttctcca tgaaaagggg
     1261 ttgcccgtag cggacttcgt tgaaatagaa tcaatatatg cattaaaaga gaagatagca
     1321 gcttttggat atccagccat gttaaaaata agaagagatg catatgatgg acggggaaat
     1381 tttagaatag aaaaacctga acatatcaca caggcttacg aacactttgg tggaaaaccc
     1441 ataatgcttg aaaaagtcat tgattttcaa atggagatat cggtgattgc agctcgaaac
     1501 ataaagggag aggttgctac atatcagcca gtagaaaaca tacacgaaga aaatatactc
     1561 aaaatgacaa tagcgccagc aagaataagt aaagaaacat ctcaagaggc agagcatatt
     1621 gccaaaaaaa ccatggaagt attacagggt gcaggcgtgt ttggaataga aatgtttgta
     1681 acacgagatg acaaagtttt aataaatgaa atagcgccgc gagttcacaa ttcgggtcat
     1741 catactttac aatcaagtgc aacttcacaa ttcgaacaac atctgagagc gatattagga
     1801 ctagaacttg gaaaaacaga tttgttacac tatgctgtca tgtgcaacat tttaggacca
     1861 aaggattttc atggaaaata ccaaccaata tcactagaag aaaaagatgg gatgtatcta
     1921 aagatgtatc aaaaagaaga agcaaaaccg caaaggaaat tgggccactt taatgtcata
     1981 gatctagaag acacaaagga catagatgca ttaatcagga aagctgaaca aattaaaagt
     2041 ttaataaaat tcaaatcagt ttgatcttat gttctattca aaaaaaccgc ttgttggaat
     2101 cataatgggt tctagctcag acagtaaaat aatgcatgga gctgcaatga ttcttgatga
     2161 gtttggagta aagcatgaag accagatagt ttcagcacat agaacaccat ctagattagc
     2221 tgattatgct aagcatgcag aaaaaagtgg cttcaaagta ataattgctg gtgcgggagg
     2281 gtctgcacat ctacctggaa tgattgcatc tcatacggtc ataccagtca taggagtacc
     2341 aataatggtg tataatgaca aatccggtag ctctgaccgt ttcaaatttt ctgcttttgg
     2401 gggtttggat tcattattgt caatatcgga gatgccaaca ggatcaccag tagtaactgt
     2461 aggggtaaac aaggcaacca atgcaggatt atatgcacta aaaattcttg ctaatgagtt
     2521 tgaggattta cagaaaaaat tgaaaaaaca caaagaaaag caacatcatt cagtgatcag
     2581 agaatctgac gaaatgaaaa aattaggctt atccaagttt gcaaagaaaa aatacaagat
     2641 gtaatattat tctgtgactt ggacataaaa gaaatactct acaactctat tgaacgacat
     2701 aaaaagtaca taatatcaga aattacacaa ggtaaaagca atgaaactat agattccata
     2761 tgtaaagaat gcatgccatt actggaaaaa cttgaaggcg ataaatcaga aatcatgatt
     2821 tcttttgcag aaggtttggt tcattatctt cttacaacat cattaattcc aagccaaaga
     2881 aaaatcaccg tagatgatac atatgttgat atttctatac ctgatattaa aacactcaaa
     2941 tccaatcccc aagatgcgtt attgataata tttccaaaaa ccaaaaatat tcaagaaata
     3001 aaaaatagag cgagtgagct taaaaaaata cagccaatcc aagagaacac atggtttgtt
     3061 cttgaaaatg atgtggatat aaaagctata gtatacagca tatacaattg gaaaattcct
     3121 ttttcacaaa tacttaatga tataatcggt ttccttacaa ataaaaaaca atcaaaactg
     3181 aaacttttta ggatataaga gacttcacat ttattttttt tccgatcata tcaaccattg
     3241 aatcaatctt attataatct tttatgaatg gtataattcc taaaagatca attccagtaa
     3301 gttcatgcaa tgttgttggc gcattttttt ctgctactgt tccctt
//
LOCUS       DATIDL010000132         3105 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrosopumilaceae archaeon isolate SMAG_U16342
            k141_165872, whole genome shotgun sequence.
ACCESSION   DATIDL010000132 DATIDL010000000
VERSION     DATIDL010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935836
            Sequence Read Archive: SRR25158326
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosopumilaceae archaeon (soil metagenome)
  ORGANISM  Nitrosopumilaceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrosopumilales;
            Nitrosopumilaceae.
REFERENCE   1  (bases 1 to 3105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/04/2024 05:09:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,520
            CDSs (total)                 :: 1,474
            Genes (coding)               :: 1,474
            CDSs (with protein)          :: 1,474
            Genes (RNA)                  :: 46
            rRNAs                        :: 1 (5S)
            complete rRNAs               :: 1 (5S)
            tRNAs                        :: 43
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3105
                     /organism="Nitrosopumilaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165872"
                     /isolate="SMAG_U16342"
                     /isolation_source="soil"
                     /db_xref="taxon:2202732"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            41..340
                     /locus_tag="VLB45_05710"
     CDS             41..340
                     /locus_tag="VLB45_05710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05233.1"
                     /translation="MGKRKFTIDLGSEKIETEGHMHKNVAIKYLMKRRRSLLMTRDKE
                     KVEKLYESVPKTISIIGGHLTKSYKINWEREGTTEFEGSRFVFTLEELPDQKIVA"
     gene            complement(371..661)
                     /locus_tag="VLB45_05715"
     CDS             complement(371..661)
                     /locus_tag="VLB45_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HSD05234.1"
                     /translation="MLLKCICDETRFKMLELLQKNKELCVNDLVSELKKDQPLVSHHL
                     KSLKQCGIVHSRESGKMTMYSIANKETSHLISDITKASEKIAAICKDPTCCA"
     gene            717..1121
                     /locus_tag="VLB45_05720"
     CDS             717..1121
                     /locus_tag="VLB45_05720"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HSD05235.1"
                     /translation="MSEVKKVLFVCVENAGRSQMAEGFFRKLASSKYQPVSAGTKPIS
                     QINPLAVQVMKEVGIDIATQKSKIITEDMIRNSSKIINMGCMDKESCPILFLNNPQDW
                     NIEDPKGKPIEKIRQIRDQIESKVKELVASLE"
     gene            1114..1794
                     /locus_tag="VLB45_05725"
     CDS             1114..1794
                     /locus_tag="VLB45_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007401177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HSD05236.1"
                     /translation="MNKFEKLSANQKRFVAELVGTFVVVVLATGSVVLDAKFTGTFGL
                     WFVALAPAVAVSLMVYAFGKVSMAHFNPAVTIGFLITRHTPKTLLALYFTAEIIGALL
                     ASLFVKYAIGTEANLGANAPNYQFSISTIIFVEILATAFLMATILVVVHTKGLKRFGG
                     VIIGSMIGLDIFFLSFISGASMNPARSLAPAIFSGAIGDLWLYLSATFIGSSIVGMIY
                     RKKFVKMP"
     gene            complement(1801..1874)
                     /locus_tag="VLB45_05730"
     tRNA            complement(1801..1874)
                     /locus_tag="VLB45_05730"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1838..1840),aa:Pro,seq:tgg)
     gene            1957..2847
                     /gene="map"
                     /locus_tag="VLB45_05735"
     CDS             1957..2847
                     /gene="map"
                     /locus_tag="VLB45_05735"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007550066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II methionyl aminopeptidase"
                     /protein_id="HSD05237.1"
                     /translation="MQIQDYINAGKIASEVRENARKKHHVGSTLYEICESIEKEIIQK
                     GGKCAFPVNTSLNEIAAHYTAEPNDQQVVQETDLLKIDLGVQINGYIADTAVTVCYDP
                     KFDYLIQAAESALKSAMSIIKAGVKSSDVGKAIESTIKQMGCIPISNLSGHSLEQYTI
                     HAGKTVPNIWSIGSFSFSPTEAYACEPFVTTREGLGFVREGKTRNIFSIVTRKRTKDA
                     EADKLLDYIWEKFNTLPFALRWLIPERDEKTARSLLEKLVKNKTIRSYPVLIEANSQR
                     VAQAEHTFIPQENGITITTI"
     gene            complement(2844..3026)
                     /locus_tag="VLB45_05740"
     CDS             complement(2844..3026)
                     /locus_tag="VLB45_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05238.1"
                     /translation="MIFSKKKEIIIPYSLEQCSSCKAISKRKFASGDYVFKNTNKCGS
                     CNGQMSITRIFGEVSA"
BASE COUNT         1022 a          560 c          608 g          915 t
ORIGIN      
        1 ggtctgcatc ctttagtaga attttgtatg atatggtctg atgggcaagc gaaagtttac
       61 tatcgacttg ggcagcgaaa aaattgaaac cgagggccac atgcacaaaa acgtagccat
      121 aaaatatctt atgaaacgac gcagatcact tttaatgaca cgcgacaaag aaaaggttga
      181 aaaactgtac gaaagcgtgc caaaaaccat atccatcata ggcggacatt tgacaaagtc
      241 ctacaagata aattgggaaa gagaaggcac aacagaattt gagggctcta ggtttgtctt
      301 caccttggaa gaactaccag accaaaaaat agttgcttag tattctaaga aaatattaat
      361 tttattatag tcaggcacag caggttggat ctttacaaat agctgcaatt ttctcacttg
      421 cctttgtaat gtcacttatc agatgtgatg tttctttgtt ggctatgcta tacattgtca
      481 tttttccact ttctcttgaa tgtactattc cacattgttt tagtgatttt agatgatgtg
      541 atactaatgg ttggtctttt tttaattcag atacaagatc atttacgcaa agttctttgt
      601 ttttctgtag gagttctaac atcttaaagc gcgtttcatc acagatgcac tttaacagca
      661 ttatactatt catattaata gacatttata taaactcata ttgatataaa agttgcgtgt
      721 ctgaagtaaa gaaagttctt tttgtttgtg ttgaaaatgc tggaagaagt cagatggcag
      781 agggattttt taggaaactt gcatcatcca aatatcaacc agtcagtgct ggaacaaaac
      841 caatttctca aatcaatccg cttgcagtgc aagtcatgaa agaagttgga atagacatag
      901 ccacacaaaa atcaaagata atcacagaag acatgataag aaattcatct aaaataatca
      961 acatgggatg catggataaa gaatcatgcc ccatactatt tcttaataat ccacaagatt
     1021 ggaacatcga agatccaaaa gggaagccaa tagaaaaaat tcgtcaaata agagaccaga
     1081 tagaatcaaa agtaaaggaa cttgttgcaa gccttgaata aatttgaaaa gctaagtgcc
     1141 aatcagaaaa ggtttgtagc tgaacttgtg ggcacatttg ttgtagttgt tcttgctact
     1201 ggttctgtgg ttttagatgc aaaatttaca ggaacctttg gcttgtggtt tgttgcactt
     1261 gctcctgctg ttgctgtatc attaatggtt tatgcatttg gcaaagtttc tatggcacac
     1321 tttaacccag cagtaacaat tggattttta attacaagac atacgccaaa gactcttcta
     1381 gctctttact ttactgcaga aataattggt gcgctattag caagtttatt tgtaaagtat
     1441 gccattggaa cagaagcaaa tcttggtgca aatgcaccaa attatcaatt ttctatatct
     1501 actatcattt ttgttgagat tttagcaact gcttttctga tggcaacaat actagttgtg
     1561 gttcatacaa agggtttgaa aagatttggt ggtgtgataa taggaagcat gataggattg
     1621 gacattttct ttttgtcttt catatctggc gcgtcaatga atcctgcaag atctttagca
     1681 cctgccatat tttctggagc gataggtgat ctgtggcttt acttgtctgc gacttttatc
     1741 ggttctagca tagtaggaat gatttacaga aagaaatttg taaaaatgcc atgaaaaaga
     1801 tgggatcggc cggatttgaa ccgacgacct caggtaccca aaacccgaat cataccaagc
     1861 tagaccacga tcccgagatg aaagcagttt ggaactcccc aatttaagct tcacatggtg
     1921 attattttaa ataatataca aaatcaagac tagccaatgc agattcaaga ctatatcaat
     1981 gctggaaaga ttgcgtcaga ggttagagaa aatgcaagga aaaaacacca tgttggttcc
     2041 acattatatg aaatttgtga atcaattgaa aaagaaataa tccaaaaagg tggcaagtgt
     2101 gcctttcctg taaatactag tctaaatgaa attgctgcac attacaccgc agagccaaac
     2161 gatcaacagg tggttcagga aacagatctg ctaaaaatcg atcttggggt ccaaataaac
     2221 ggctacattg cagacactgc cgtaactgtg tgttatgatc caaaatttga ttatcttatt
     2281 caagccgcag aatctgcgct aaaaagtgca atgtcaatca taaaagcagg agtgaaatca
     2341 agtgatgttg gaaaggcaat agagagcaca atcaaacaga tgggctgcat cccaatatca
     2401 aatctaagtg gacattcatt ggaacaatat accatccatg caggaaagac agtaccaaac
     2461 atctggtcca tagggtcttt ctcattttct cccacagaag catatgcgtg tgagccattt
     2521 gtcacaaccc gcgagggatt aggatttgtg cgcgaaggca agacaagaaa catcttttct
     2581 attgtcactc gaaagcgaac aaaagatgca gaagctgaca agctactcga ctatatctgg
     2641 gagaaattca acactttgcc ttttgctttg agatggctga taccagagcg ggatgaaaaa
     2701 acagctagaa gtcttttgga aaagcttgta aaaaacaaga ccataagatc atatcctgta
     2761 ctaattgagg caaatagtca gagggtcgca caggctgaac acacgtttat cccacaagaa
     2821 aacggcatca ctataactac aatctaggca gaaacttcgc caaaaattct ggtaatggac
     2881 atttgtccgt tgcatgaacc gcacttgttt gtgtttttga acacataatc tccgctggca
     2941 aattttcttt ttgatattgc cttgcaagaa ctgcattgtt ccaaagaata tggaattatg
     3001 atttcttttt tctttgaaaa gatcattgtg atactcctag agtgtttccc actccaatta
     3061 caagcaccga ttgcccgctc tttgtgtttt ccttgattgt ttcat
//
LOCUS       DATIDL010000133         2696 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrosopumilaceae archaeon isolate SMAG_U16342
            k141_2588870, whole genome shotgun sequence.
ACCESSION   DATIDL010000133 DATIDL010000000
VERSION     DATIDL010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935836
            Sequence Read Archive: SRR25158326
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosopumilaceae archaeon (soil metagenome)
  ORGANISM  Nitrosopumilaceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrosopumilales;
            Nitrosopumilaceae.
REFERENCE   1  (bases 1 to 2696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/04/2024 05:09:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,520
            CDSs (total)                 :: 1,474
            Genes (coding)               :: 1,474
            CDSs (with protein)          :: 1,474
            Genes (RNA)                  :: 46
            rRNAs                        :: 1 (5S)
            complete rRNAs               :: 1 (5S)
            tRNAs                        :: 43
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2696
                     /organism="Nitrosopumilaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2588870"
                     /isolate="SMAG_U16342"
                     /isolation_source="soil"
                     /db_xref="taxon:2202732"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..713
                     /locus_tag="VLB45_05745"
     CDS             <1..713
                     /locus_tag="VLB45_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HSD05239.1"
                     /translation="EYGSTITTNKGKYVYLLDPTIHDYVMKSQRSTQIVYPKDLGYIA
                     ARTGLQSGQKIVEIGTGSGSLTTFLASIVKPRGHVYTYDVDPNFMDIARKNIEKAGIA
                     KYVTMEKFDMKTAKKVPQKDVDLVVVDLGDPWSVVPQARKMLKGSGAFVAICPTMNQL
                     EKLATALRENDFFDIECTEQLVRTIEAREGKTRHSFRSIGHTTYVAFARKVYSPKRKI
                     IAVDKQDSEEPDSAEPDS"
     gene            679..1542
                     /locus_tag="VLB45_05750"
     CDS             679..1542
                     /locus_tag="VLB45_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HSD05240.1"
                     /translation="MKSQTLQSQIVKKFIPPRKFLSRKGDNGKVLVLGGSYLYHGAPI
                     LSSVAALRCGTDLVYTCVPKINVQATRSFSPNLIVLPLVDAKLTRGAVHKLLGIIPKD
                     LDSATIGMGLAIQDIEALKLLVTTLLNRNVRLSLDASALLPDILPLISNKNVVVTPHA
                     GEFKRLFGQLPLDSKKERIAIVEKYAKANSVTVLLKGPTDIVSDGNKTFLNVKNTPAM
                     TVGGTGDVLSGLVAGMLSKNKNPLESAAAAVFVNGKAGTLAQRKYGLHMAATDLLEFI
                     PAAMKPFDRVK"
     gene            1548..>2696
                     /locus_tag="VLB45_05755"
     CDS             1548..>2696
                     /locus_tag="VLB45_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HSD05241.1"
                     /translation="MKVLGHVDHNMKGLVSDLQTLIRQPSVSAKNEGLEECARLVAKI
                     MRKSGIKSEILRLGKGIPPVVYGEVRSKQNNAKTILFYNHYDVQPAEPFELWDDKPFS
                     GKIKGNKIFGRGSADDKGELITRIKAVEAYLKVHGDVPCNIKFLVEGEEEIGSMHVEK
                     YLKKYQKKLACDGVIWEFGYVDQKNRPIISLGMKGLLYVELSVSESIRDAHSSLAVLI
                     KNPAWRLLEALKTLRNSDGKILIKDWYKEVDRFTKEDLALIKSEPFDENSFKKEYGIK
                     QFVANKKGTDAKTALVGEPTCNIAGFVSGYIGPGAKTVLPAKALVKIDFRLVPKMNPK
                     KQLARLKQHLRSNGFGEVQVKMIHGEAASRTKTSDPFVTQVEGAANESF"
BASE COUNT          938 a          476 c          583 g          699 t
ORIGIN      
        1 aagagtatgg ttccacgata actaccaaca aaggcaagta tgtctatctt ttagatccta
       61 ccattcatga ttatgtgatg aaaagccaac gcagtactca gatagtgtat ccaaaagatc
      121 tgggatacat tgcagcaaga actggattgc aaagcggaca aaaaattgtt gaaataggta
      181 ctggcagtgg ttcacttacg acatttcttg caagtattgt aaaaccgcgt ggtcatgttt
      241 acacatatga cgttgatcca aactttatgg acattgcaag aaaaaatatc gaaaaagctg
      301 gaattgcaaa atatgtgaca atggaaaaat ttgatatgaa gacagcaaag aaagtaccgc
      361 aaaaggacgt ggatttggtt gttgttgatc ttggtgaccc atggtctgtt gtaccgcaag
      421 caagaaagat gctaaaggga agtggcgcat ttgtagcaat atgtccaacc atgaatcagc
      481 ttgaaaaact tgcaactgct ctacgagaaa atgatttttt tgatatagaa tgcacagaac
      541 aattagttag aacaatcgaa gcacgagaag gaaagacacg ccactcattt cgtagcatag
      601 gtcacactac atacgttgct tttgcaagga aggtttattc ccctaaaaga aagattatcg
      661 ctgttgacaa gcaagattct gaagagccag actctgcaga gccagatagt taaaaaattc
      721 atacctccaa gaaagtttct atcacgaaag ggcgataacg gtaaagtctt ggtgcttggt
      781 ggtagctacc tgtaccatgg tgctccaata ctgtcatctg ttgcagcact acgatgtggc
      841 acagatctag tgtatacatg tgtgccaaaa ataaatgtgc aagcgacaag atctttttct
      901 cctaatctta ttgttcttcc actagttgat gcaaaactga cacgtggtgc agtacacaaa
      961 cttcttggaa tcattccaaa agatcttgat tcagctacta ttggaatggg acttgcaata
     1021 caagacattg aagcattaaa acttcttgta accacattac tcaatagaaa tgttagactc
     1081 tcacttgatg caagtgcatt gttgcctgac attttgccct tgataagtaa caaaaatgtt
     1141 gtagtcacac cacatgcggg agaattcaaa agattatttg gtcaactgcc gctagactca
     1201 aaaaaagaaa gaatcgctat tgtggaaaaa tacgcaaaag caaactcagt cacagtatta
     1261 ctaaaaggcc caactgacat tgtatccgat ggtaacaaga catttcttaa cgtgaaaaac
     1321 actccggcaa tgacggtggg aggaacaggc gatgtacttt ctggccttgt ggcaggaatg
     1381 ctgtcaaaaa acaagaatcc actagaatct gctgcagcgg cagtatttgt caacggaaag
     1441 gctggtacac tagcacaacg aaagtatggt cttcacatgg ctgctaccga tctcttggaa
     1501 tttattccag ccgcaatgaa accatttgat agggtaaagt gaatcagatg aaagtactag
     1561 gacacgtaga tcataacatg aaaggactag tttctgatct gcaaacccta attcgacaac
     1621 caagtgtttc tgcaaaaaat gagggtctag aggaatgtgc tagacttgtt gcaaagataa
     1681 tgagaaagtc tggaataaaa tctgaaatac tgcgtcttgg aaaaggcata ccgcctgttg
     1741 tatacggtga ggtaagatca aaacaaaaca atgcaaaaac aattttgttt tacaatcatt
     1801 atgatgtgca gccggcagaa ccttttgagc tgtgggatga taaaccattt agtggaaaaa
     1861 taaaaggaaa caagatcttt ggacgaggct ctgctgatga caagggtgaa ttaatcacta
     1921 gaataaaagc agtagaagct tacctgaaag ttcatggtga tgttccatgc aacatcaaat
     1981 ttcttgttga aggcgaagaa gaaattggaa gcatgcatgt ggaaaaatat ctgaaaaaat
     2041 accaaaagaa actagcatgt gatggtgtga tctgggagtt tggttatgtt gatcaaaaga
     2101 acagaccgat aatcagcctt ggaatgaagg gattgcttta cgtggaactt tctgtaagcg
     2161 aatcaattcg tgatgcacat tctagtttgg cagttcttat aaaaaatcct gcatggagac
     2221 ttttagaagc actaaaaaca ctacgcaact ctgatgggaa gatactcatt aaagattggt
     2281 acaaagaggt agaccgtttt acaaaagaag atcttgctct tataaaatct gagccatttg
     2341 atgaaaattc attcaaaaaa gaatatggaa taaagcagtt tgtggcaaac aagaaaggaa
     2401 cagatgcaaa aacggcgctg gtaggagaac ctacgtgtaa catagcgggt tttgtatctg
     2461 gatatattgg ccctggggct aaaacagttc ttcctgcaaa ggcccttgta aaaatcgact
     2521 ttagacttgt gccaaagatg aatccaaaaa aacagcttgc aagactaaaa cagcatctta
     2581 gatcaaatgg ttttggtgaa gtgcaagtga aaatgattca tggcgaagct gcatctagaa
     2641 caaaaacctc tgatccgttt gttactcaag tagaaggtgc tgcaaacgaa tcattt
//
LOCUS       DATIDL010000134         4240 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrosopumilaceae archaeon isolate SMAG_U16342
            k141_573185, whole genome shotgun sequence.
ACCESSION   DATIDL010000134 DATIDL010000000
VERSION     DATIDL010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935836
            Sequence Read Archive: SRR25158326
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosopumilaceae archaeon (soil metagenome)
  ORGANISM  Nitrosopumilaceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrosopumilales;
            Nitrosopumilaceae.
REFERENCE   1  (bases 1 to 4240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/04/2024 05:09:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,520
            CDSs (total)                 :: 1,474
            Genes (coding)               :: 1,474
            CDSs (with protein)          :: 1,474
            Genes (RNA)                  :: 46
            rRNAs                        :: 1 (5S)
            complete rRNAs               :: 1 (5S)
            tRNAs                        :: 43
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4240
                     /organism="Nitrosopumilaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_573185"
                     /isolate="SMAG_U16342"
                     /isolation_source="soil"
                     /db_xref="taxon:2202732"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..858)
                     /locus_tag="VLB45_05760"
     CDS             complement(<1..858)
                     /locus_tag="VLB45_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HSD05242.1"
                     /translation="MSGKRIVLTADRSLMTNYRGNFLYGFIACGPYEVLPEWVFDKVF
                     CPSVETDKVTGEAKVAQVGLRRVESALLQGYNRDEVFVAQPEYLANCVGPDTKVVGIN
                     VMDPLGMAPVTTTMSPEKLSYVAMKFKRMCADIIQLKKKYNFHVVVGGNGAWELAKSD
                     RMKIHGIDTVVVGEADELALDLFHDLEKGDAPELMHCFVKNIQNIPIIQGPTVNSLIE
                     AMRGCGRGCDFCDVNKRSKKDLPLERLQHEAKINLDYGFDSIWLHSDEMLLYGCDNKD
                     FQPNYDAITG"
     gene            942..1664
                     /locus_tag="VLB45_05765"
     CDS             942..1664
                     /locus_tag="VLB45_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008300229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HSD05243.1"
                     /translation="MKDVAEPRHVERHQTETSAIRDFVFGFGDGINTSLGIVAGVGGA
                     DISSNIIILAAIVAMFTGAKAMAVQNYLAVKSQREILHSEIEREKWEMENVPEKERQE
                     IRDIYKAKGFSGNELETIVNKITSDKKVWLDTMLTEELKLNLEIVGSPLKSALRMFGS
                     FLIGGILPIVPYFFADGYIPLFIAIGVSLSSSFVIGAIKSKIANLSMIKGGLEMAGLG
                     TGIALIGYGIGSELANLGIVNV"
     gene            complement(1661..2407)
                     /gene="larB"
                     /locus_tag="VLB45_05770"
     CDS             complement(1661..2407)
                     /gene="larB"
                     /locus_tag="VLB45_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HSD05244.1"
                     /translation="MGKISVMKAKKLLALHAVEKIEDLVKLDVGRKFRRGIPEVIFAE
                     KKQLAEIKKIILTIMRRSDSVFVSRINKNDYEKILSFSKKNKIKKKTGKNTTAVLFFK
                     KSLNSSGGRVGILTAGTSDIGVAEEARLMCEAMGCTCISSYDVGIAGLHRVLPVIKKM
                     IKEEVDAIIVVAGMEGALASIVSSLVDIPVIGVPSSVGYGYGEKGVAALAAMLQSCTL
                     GLSVVNIDNGIGAGAIAANIANRSMIKKRN"
     gene            complement(2476..3390)
                     /locus_tag="VLB45_05775"
     CDS             complement(2476..3390)
                     /locus_tag="VLB45_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010191989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HSD05245.1"
                     /translation="MITIIGAGKVGGAAALFTALRKLSDEILLLDIVQGLPQGEAMDL
                     NHMLSEKGIDVNIRGSNDYSEMKGSDIVVVVAGSGRKPGMTRMDLLKINTSIVKGVVE
                     NIKKYANDSMIVPVTNPLDPMAYLAYKVSGFEKNRIFGMGNMLDLSRFKQFIHEATGF
                     SRHSIGALVIGEHGENMLPLARYSSISGIPLTSILSKQKIEEIVQNTRQVAAKVIELK
                     GATVHAPGNAISAIIEAVIRNTKEVIPVATYLDGEYGQSGVSIGVPAVIGKNGVEKIV
                     EVELDSDEKEWFKKGIDSVKTAISSMEI"
     gene            complement(3483..3686)
                     /locus_tag="VLB45_05780"
     CDS             complement(3483..3686)
                     /locus_tag="VLB45_05780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05246.1"
                     /translation="MCIIGDYRQIIAQREMGLVYSEMKVPRKKINRELQHNPGLKPIY
                     GYMDTRTEIKRRLLKRKVLEIIS"
     gene            3920..>4240
                     /gene="larC"
                     /locus_tag="VLB45_05785"
     CDS             3920..>4240
                     /gene="larC"
                     /locus_tag="VLB45_05785"
                     /EC_number="4.99.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF014072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel insertion protein"
                     /protein_id="HSD05247.1"
                     /translation="MVVVIDSQVAGISGDMLLSALIDIGAKKSKVIDGIHIAENYLTG
                     SRIQKIDFGSVTKHGTKATELLLDIREDTHERKGIEIQECILLTSDKIGLSEKSKVFA
                     KESIK"
BASE COUNT         1324 a          800 c          702 g         1414 t
ORIGIN      
        1 tccagttatt gcatcatagt ttggctgaaa gtctttattg tcacatccat agagaagcat
       61 ctcatcagaa tgcaaccaaa tcgaatcaaa cccataatct agatttatct ttgcttcatg
      121 ttgtagtctt tccaatggaa gatctttttt ggatcgtttg tttacatcac aaaaatcaca
      181 tcctcttcca catccacgca ttgcttcaat taaggagttg actgtaggtc cctgaatgat
      241 tggaatgttc tgaatatttt tgacaaaaca atgcattaat tctggagcat cgcctttttc
      301 caaatcatgg aataaatcaa gagcaagttc atctgcttct cctacaacaa cagtatcaat
      361 tccatgaatc ttcatcctat cagattttgc aagctcccat gctccatttc ctccaaccac
      421 aacatggaaa ttatattttt tctttaactg aattatgtct gcacacattc ttttgaattt
      481 cattgcaacg taagagagct tttctggtga catcgtagtg gttactggtg ccatgccaag
      541 aggatccatc acattgattc ctacaacttt ggtatcaggt cctacacaat ttgccaaata
      601 ttctggttgc gcaacaaaaa cttcatctct attgtatcct tgcaagagtg cactttctac
      661 tcttcgaaga ccaacctgtg ctacttttgc ttcccctgta actttatcag tttcaacact
      721 agggcaaaag actttgtcaa agacccattc aggaagaacc tcatatggac cacatgcaat
      781 aaagccgtaa agaaagttac ctctatagtt tgtcataaga ctcctgtcag ctgtaagaac
      841 tatccttttt ccagacattt tctgtagtat ctccttttac tatgatataa atcgtttacg
      901 acaagagact tgaaatgtct ggtcatttca tgtgaataga catgaaagat gttgcagagc
      961 caagacatgt agaaagacat cagactgaga caagtgccat aagagacttt gtctttggtt
     1021 ttggtgatgg gattaacact tctttaggca tagttgctgg tgtcgggggt gctgatattt
     1081 cttcaaacat aatcatactt gcagcaatag ttgccatgtt cacaggtgca aaagcaatgg
     1141 cggtacaaaa ctacttggcg gtaaagtctc aaagagagat attacattct gaaatagaac
     1201 gagaaaaatg ggaaatggaa aatgtacctg agaaagaaag gcaagaaatt agagacattt
     1261 acaaagccaa aggattttct gggaatgagc ttgaaacaat tgtgaataaa attacttcag
     1321 ataaaaaagt gtggttagat acaatgctta ctgaagaatt aaaattgaac ttagaaattg
     1381 ttggtagtcc tctaaaaagt gcattaagga tgtttgggtc tttcttgatt ggtggaatac
     1441 tacctatagt gccatacttc tttgctgatg gctacatacc actttttatt gcaataggag
     1501 ttagtctttc ttcgtcattt gtaataggag ctatcaaatc aaagattgca aatctcagca
     1561 tgataaaagg tggattagag atggcaggac ttggcactgg aattgcattg attggatacg
     1621 gcatcggatc ggaacttgca aatcttggca tagtgaatgt ttagtttctt ttcttaatca
     1681 tagatctatt tgcaatatta gctgcaatgg ctccagcacc tattccatta tctatattta
     1741 cgacagacag tccaagagtg caactttgaa gcattgcagc aagagcagca acaccttttt
     1801 cgccataacc atagccaaca gaagatggaa caccaattac aggaatgtct acaagtgagg
     1861 acactatgga agctagtgcc ccttccattc cagcaactac aatgattgca tcaacttctt
     1921 cttttatcat tttttttatg actggcagaa cacggtgcaa tccagcaatt cccacatcat
     1981 agctagaaat gcaagtacac cccattgctt cacacatcag tcttgcttct tctgctactc
     2041 caatatctga agtaccagca gtcaatattc ctacacggcc accagatgaa tttaatgatt
     2101 ttttgaaaaa taaaacagca gtagtatttt ttccagtttt tttcttaatc ttatttttct
     2161 ttgaaaaaga tagtattttt tcatagtcat ttttgtttat tcttgataca aatacagaat
     2221 cagatctacg cataatagtt aaaataattt tttttatttc agcaagttgt tttttttctg
     2281 caaaaattac ttcaggtata ccacgcctaa attttcttcc aacatcaagc tttacaagat
     2341 cttcaatttt ttctacagca tgtaaggcaa gaagtttttt tgctttcatt accgaaattt
     2401 tacccatgtt tagagactcc aagatttcta ttaaattcaa cgatgtttct aatccagata
     2461 cagttaaaaa tttaattaga tttccatgct agatatggca gttttaacgc tgtcaattcc
     2521 ttttttgaac cactcttttt catcagaatc aagttcaact tcaactattt tttctacgcc
     2581 atttttacca atcactgcag gcacacctat ggacacaccc gactgaccgt attcaccatc
     2641 aagatacgtg gctacaggta tcacctcttt tgtgtttctg ataactgctt caattattgc
     2701 agaaatcgcg tttccaggag catggaccgt ggcgcccttt aattctataa ctttggcagc
     2761 aacctgcctt gtattttgta caatctcttc aatcttttgt tttgacagta tagatgtaag
     2821 tggaattcca gatattgatg aatatcttgc taagggcaac atgttttcac catgttctcc
     2881 aatcaccaac gcccctattg aatggcgaga gaatccagtt gcctcatgta tgaattgctt
     2941 gaatcgcgat agatcaagca tgttgcccat tccaaatatt ctattttttt caaatcccga
     3001 cactttgtaa gcaagatatg ccataggatc aagcgggttt gtcacaggaa cgatcataga
     3061 atcatttgca tattttttga tattttcaac aacgcctttt acaatagatg tatttatttt
     3121 caaaagatcc attcttgtca ttccaggttt tcttccagag cctgcaacca caacaacaat
     3181 atcagaacct ttcatttcag aataatcatt ggaaccacgt atgttcacat caattccttt
     3241 ttcagataac atatgattta gatccatagc ctctccttgt ggaagtcctt gtacaatgtc
     3301 tagaagaaga atttcatcag aaagttttct aagtgcagta aaaagtgcag ctgcaccacc
     3361 tactttccca gctccaataa ttgtaatcat aaacaggcgt aattttaatc gttaattaaa
     3421 cttctcgatt tgaataattt ttgacaaaag aaaactagat gtcagatatg aaattctaat
     3481 gattatgata tgatttctaa cactttgcgc ttgagtagtc ttctttttat ctcagttctt
     3541 gtgtccatgt aaccgtatat cggttttagt ccaggattat gttgtagttc tctgttgatt
     3601 ttttttctag gtactttcat ttcagaataa accaagccca tttcgcgttg tgcaattatt
     3661 tgcctgtaat caccaataat acacatgtga gatttactca tacgcaatta tttaagtatg
     3721 tttttttaag attcccatat tataaattgc caattataaa aaaatagcat gttttatttg
     3781 taaattatga atttctttaa ttatacttgc tacaattttt atagtaaata attgatttgg
     3841 aatcaaaatg agttcaattt ttaatcgcat tatgaattaa aattacaatt tatatgcatt
     3901 ctaagaggtt tgcactttca tggtagtggt gatagattca caagtagctg gtatttcagg
     3961 ggacatgtta ctttctgcct tgatagatat tggagcaaaa aaatcaaaag ttatcgatgg
     4021 aatacatatt gcagaaaatt atctgacagg atctagaatc cagaaaatag attttggcag
     4081 cgtaacaaaa catggaacaa aagctacaga acttttgtta gatataagag aagatacgca
     4141 tgagcgaaaa ggaattgaaa ttcaagaatg tatcctgctt acttcagaca agattgggtt
     4201 atctgaaaaa tccaaagtct ttgcaaaaga gagtataaag
//
LOCUS       DATIDL010000135         2472 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrosopumilaceae archaeon isolate SMAG_U16342
            k141_975086, whole genome shotgun sequence.
ACCESSION   DATIDL010000135 DATIDL010000000
VERSION     DATIDL010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935836
            Sequence Read Archive: SRR25158326
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosopumilaceae archaeon (soil metagenome)
  ORGANISM  Nitrosopumilaceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrosopumilales;
            Nitrosopumilaceae.
REFERENCE   1  (bases 1 to 2472)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2472)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/04/2024 05:09:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,520
            CDSs (total)                 :: 1,474
            Genes (coding)               :: 1,474
            CDSs (with protein)          :: 1,474
            Genes (RNA)                  :: 46
            rRNAs                        :: 1 (5S)
            complete rRNAs               :: 1 (5S)
            tRNAs                        :: 43
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2472
                     /organism="Nitrosopumilaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_975086"
                     /isolate="SMAG_U16342"
                     /isolation_source="soil"
                     /db_xref="taxon:2202732"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1793
                     /locus_tag="VLB45_05790"
     CDS             <1..1793
                     /locus_tag="VLB45_05790"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HSD05248.1"
                     /translation="LSYKGENTKILDLRNRTVIPGLNDSHIHIIRGGLNYNLEMRWDG
                     IPTLNDALSRIEKQAERTPNPHWVRVVGGWSEFQFTEKRMPTIQEIEKYAKNIPAFIL
                     HLYHCAMINKAAIDVVGYNKDTPNPPGAEFQRDISSNLTGLITAKPAATILYQTLAKG
                     PKLSYEEQVNSTRQFMYELNRFGVTSAIDAGGGFQNYPDDYKVIDDLAEQDLLTVRIA
                     YNLFTQKPKKELEDFTNWIKIAKPGQGSDFYKLNGAGEMLVFSAADWENTLEPRPELA
                     EQMESELKEVIKVLVQNRWPFRMHATYNESITRFLNVFEEVNNEIPFDGLRWFFDHAE
                     TITEENIQRVKALGGGIAIQDRLAFQGEYFIERYGKEVSENSPPLKKIIEIGVPLGAG
                     TDATRVSTYNPWISLYWLVSGKSVGGTILRSQNNCLDRMEALRLYTLGSAWFSNEEEK
                     KGSIEVGKFADLVVLTDDYFSIDEEKIKNIQSVLTIVGGKVVYATNEFQNLAPQPLSI
                     KPSWSPVAAFGGYYNIRQEKNDIEEGELAIPYNTQQLESNNKLNYKKDENIKSTLSLL
                     STHLDKNTNTYNISNMRKGLCSCCDDFFSF"
     gene            1833..>2472
                     /locus_tag="VLB45_05795"
     CDS             1833..>2472
                     /locus_tag="VLB45_05795"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSD05249.1"
                     /translation="MTDDTNTQKIANKEEKKISGFSPLNHPIYKTLWFATIGSNIGNA
                     MHDVGALWLMTNITDSPLMVALVQTSTALSMVLLSLFAGGLADVVDRRKLLLITQGYM
                     FVVAMTLGFLTIGELVTPTTLLLLTFMLGAGAAISIPSSIAITLEMVSKPEIPATITL
                     GGVSLNIGRAVGPAIFGFIIASLANPGFVFILNALSFIVLIIALSRWHPSHEK"
BASE COUNT          866 a          386 c          454 g          766 t
ORIGIN      
        1 gcctttctta caaaggggaa aatacaaaaa tacttgattt gagaaatcgt acagtaattc
       61 caggtttaaa cgactcccat attcacatta tacgtggtgg attgaattac aatctagaga
      121 tgcgatggga tggaatacca acattgaatg atgctttatc aagaattgaa aaacaagcag
      181 aacgaacacc aaatcctcat tgggttagag tagttggagg ttggtcagag tttcaattta
      241 ctgaaaaaag aatgcctact attcaagaaa ttgagaaata tgcaaagaat attcctgcat
      301 ttattttaca cctttatcat tgtgctatga ttaataaagc tgcaattgat gttgttggat
      361 ataataaaga tacaccaaat cccccaggcg ctgagtttca gcgcgatatt tctagtaatc
      421 taacaggttt aattactgca aaacctgcag caactatact ttatcaaaca ttagcaaaag
      481 gtcccaaatt gtcctatgaa gaacaagtaa attctaccag acaattcatg tatgagctaa
      541 atcgttttgg tgtcactagt gctatagatg caggaggagg ttttcaaaat tatccagatg
      601 attataaggt aattgatgat ttagcagagc aagatctcct tacagttcgc attgcttata
      661 atctctttac tcagaaacca aaaaaagaac ttgaagattt tacaaactgg atcaaaatag
      721 caaaaccagg acaaggaagt gatttttata aattaaacgg agctggtgaa atgcttgtgt
      781 tttcagcagc agattgggag aatacacttg aaccaaggcc agagctagca gaacaaatgg
      841 aatctgaatt aaaagaagtc attaaagttt tagtacaaaa tcgctggcct tttagaatgc
      901 atgctaccta taatgaatct attactcgct ttttaaatgt ctttgaagaa gtaaataatg
      961 agattccctt tgatggtcta agatggtttt ttgatcatgc agaaacgata actgaagaaa
     1021 atattcaaag agtgaaagca ttaggtggtg gtatagcaat acaagataga cttgcattcc
     1081 aaggtgaata ttttattgaa agatatggga aagaggtatc agaaaactct ccacctttaa
     1141 agaaaataat tgaaattggt gttcctcttg gagctggtac agatgccact agagtgtcta
     1201 catataatcc ttggatttcg ctttattggc tagtttcagg gaaatcagtt ggtggtacaa
     1261 tattaagatc tcaaaataat tgtcttgata gaatggaagc ccttagattg tacacgctcg
     1321 gtagtgcatg gttttcaaat gaagaagaga aaaaaggttc tattgaagtt ggaaaatttg
     1381 cagatcttgt agtattgaca gatgattatt tttcgataga tgaagaaaag ataaagaata
     1441 ttcaatcagt attaacgatt gttggtggga aagtagttta tgcaacaaat gaatttcaga
     1501 atttagcacc acaaccttta agtattaagc cttcatggtc accagtggca gcttttggtg
     1561 gatattataa tattagacaa gaaaaaaatg acatcgaaga aggagaatta gcaataccat
     1621 ataatactca acaattagaa tcaaacaata aattaaacta taaaaaagat gaaaatatta
     1681 aatcaacatt atcactatta tctactcatt tagacaaaaa tacaaataca tacaatatat
     1741 ctaatatgcg aaaaggttta tgttcatgct gtgacgattt tttctcattt taaaacatat
     1801 ttttagtcaa aaaatgatat tatcatacca caatgactga cgatacaaat acccaaaaaa
     1861 tagcaaataa agaagaaaaa aaaatatccg gcttttctcc cctaaaccat cctatctata
     1921 aaactctatg gtttgctaca attggatcaa atattggtaa tgcaatgcat gatgttggtg
     1981 cactttggtt aatgaccaac attactgact cacctcttat ggtagcacta gtacaaactt
     2041 caactgctct atctatggtt ttattgtcac tttttgctgg aggtttagca gatgtagtag
     2101 atcgcagaaa actgcttttg ataacgcaag ggtacatgtt tgttgttgcc atgactttgg
     2161 gatttttgac tattggagaa cttgttacgc ctactactct gttattgctt acttttatgt
     2221 taggtgcggg agctgcaata agtattccat catcaatagc cattactcta gaaatggttt
     2281 caaaacccga aatacctgca acaattacac ttggaggtgt ttcattaaat attggacggg
     2341 cagtgggacc agctatcttt ggatttataa tagcatcatt ggccaatcct ggatttgttt
     2401 ttattttaaa cgctttgtct ttcatagtct tgatcattgc attgtctaga tggcatccat
     2461 ctcatgagaa ga
//
LOCUS       DATIDL010000136        11331 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrosopumilaceae archaeon isolate SMAG_U16342
            k141_2590733, whole genome shotgun sequence.
ACCESSION   DATIDL010000136 DATIDL010000000
VERSION     DATIDL010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935836
            Sequence Read Archive: SRR25158326
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosopumilaceae archaeon (soil metagenome)
  ORGANISM  Nitrosopumilaceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrosopumilales;
            Nitrosopumilaceae.
REFERENCE   1  (bases 1 to 11331)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11331)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/04/2024 05:09:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,520
            CDSs (total)                 :: 1,474
            Genes (coding)               :: 1,474
            CDSs (with protein)          :: 1,474
            Genes (RNA)                  :: 46
            rRNAs                        :: 1 (5S)
            complete rRNAs               :: 1 (5S)
            tRNAs                        :: 43
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11331
                     /organism="Nitrosopumilaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2590733"
                     /isolate="SMAG_U16342"
                     /isolation_source="soil"
                     /db_xref="taxon:2202732"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(215..721)
                     /locus_tag="VLB45_05800"
     CDS             complement(215..721)
                     /locus_tag="VLB45_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008301390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide-binding protein"
                     /protein_id="HSD05250.1"
                     /translation="MVSRVLDASAFYAGVPFSSPEKCYTTSIVFDEIKHIKKHHDAVN
                     ALIDTNRLEVKDPEIEYVETVSLKAKDVGDFQNLSQGDLSVIALCLQMHAELISDDFA
                     VSNTAKHLGINVIPVMTKGITRVIEWTYFCPGCEKIFSKASQCPLCGNKLSRKLDKRK
                     SSVNPIRK"
     gene            complement(711..1526)
                     /locus_tag="VLB45_05805"
     CDS             complement(711..1526)
                     /locus_tag="VLB45_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007549856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HSD05251.1"
                     /translation="MDLNRVAVVSKVGSKESEDAAKKVAKKLLGKKTQVFTISPVEVD
                     GAKKVSTLEELKTAKLDLTITLGGDGTTLRAFRYLENEVPLLAINVGGNRGILSEITL
                     NQIDTAIEQIISGNIFLDKRIRVVASCKGQEYPPALNEIYINRQNLTKTSLFEIRFQN
                     DTVTQKMDGVIVSTPSGSTGHSYSLGGPILHESLDVLIITPVAPVNRLPSLVVPDEKI
                     EIINSHDSNIIMDAQVVKPASYDDVITIRKYKKPAVFVRLKKRGLRQMSKLGF"
     gene            1612..2457
                     /locus_tag="VLB45_05810"
     CDS             1612..2457
                     /locus_tag="VLB45_05810"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008301359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HSD05252.1"
                     /translation="MSYANPNVLTDTDWVSKNISNKAIKIVEVDYDPENAYRQGHIQQ
                     ASLIWWKRDINDPITRDIINKTQFEELMGRNGILPENEVVLYGDFNNWFAAFVFWIFK
                     YYGHKNVKIMNGGRKKWELEKKQYTKDEPQIAKTKYVAQPPDEGLRAYLFDVRRSLDK
                     QDSILVDVRSAKEYTGEITAPPEYPMEHAQRGGHIPSAKNIPWATAVNDADGTFKSVN
                     ELKQIYESKNVTPDKDVICYCRIGERSSHSWFVLKYLLGYPQVRNYDGSWTEWGNMIG
                     NPVEK"
     gene            2459..2764
                     /locus_tag="VLB45_05815"
     CDS             2459..2764
                     /locus_tag="VLB45_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05253.1"
                     /translation="MSQELPLLKQGIFYFIRDGIQDIIMEDKTKRGLTVQERSLDENY
                     HVEAEKGMIYDMDGIGHRVCIRWYFPKKDYDLGKVLLIAEEMEKKYKKLREETCPDY"
     gene            2905..4923
                     /locus_tag="VLB45_05820"
     CDS             2905..4923
                     /locus_tag="VLB45_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase family protein"
                     /protein_id="HSD05254.1"
                     /translation="MAINPLTQFLFSVFITIAVIVTLYTCNFYYLVFLSRQRRKSQKT
                     DLLGTPIVTIQLPIYNERYVAARLVNSVCSMDYPKEKMCIQVLDDSDDDTYDILENLI
                     QDYKKKGFDISHIRRENRKGYKAGALRNAMKFAKGNFIAIFDADFIPPSWFLKKAIPY
                     FAQSEIGLIQCKWGHVNEKYSALTQAQALSLDFHFLIEQKAKSNSHLYMSFNGTAGIW
                     RKECIEDAGGWHTATLVEDLDLSYRAQMKGWKCLFLPDIVVDAELPVQMNAAKRQQFR
                     WAKGSIQCAIKLMGDVVVKKIPFDTKIQAFVQLTRHIVHPLMLIQFLILPILLQAKFN
                     LYVISVLPLLTIVAYLAMGPLVYIMIIRNLWQQSWKSKTLAYFYLLFYSAGMSVNNTV
                     AVFDALLGKKNEFLRTPKYGIVKKSDDWRDKAYNLPFTKTTLLEIFFGIYGVLGIFVA
                     IFSGNPVFVPLIALQTLGFIYISYLSVSHTTFKKNKSRSLHNTSKAEKMANAYYKLAM
                     VGIVGFIAFGAFMAYYGYQNDVYPLDQSRGLLDRIQATSDPQTIIDDLKTMKILLPED
                     GNPVWIFPTETTDFGRIQDDIDTMILSVEKISTVPQDSSSFHTGMLDVHERAFKMRLH
                     LMDATPYMYVSFSNIVFSCIWIAAIMGIFTMLKRKKDRLKAFEMSDEV"
     gene            complement(4920..6188)
                     /locus_tag="VLB45_05825"
     CDS             complement(4920..6188)
                     /locus_tag="VLB45_05825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HSD05255.1"
                     /translation="MAKHSSRTSNSKRKTVRDSSSDVLKKMNSISDATKILANEVKSM
                     SKIFAENQKILISLKNMIDAVGSSLEQIQRNARQINVLEEDTQKLFTGLSQIKAHSNL
                     ISKVNDQTDRLQDQVNKIREKQESLPDVNKVMQSVANSLDSIRNNTKMIMHVSDKTEK
                     IRDEIKDVSAKAEKITQLEQNITNLKQDIGAITSKTESLVNMSSDIRDVGIELGSLVR
                     KTDSLATFGADIRNVGTEFTNFRENVLGKTKQIDEKISELTELLNRNSAATSEFHKRT
                     HDIHQELQEIRNITHKTSQNTSREVVGLLRLAEFQSNIRMHSESKYGTLDDLDKMISQ
                     TVDMVNLFDRLSLESEKKIPLPQEVKQWSVSKILDCADRWEIRFTDVFRLLINQLGRD
                     LIKESMRIEQVRDLFGIRAVDEIRTELGIP"
     gene            6616..7173
                     /locus_tag="VLB45_05830"
     CDS             6616..7173
                     /locus_tag="VLB45_05830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05256.1"
                     /translation="MSSEILEKVASAGIDGIKRIELKRTFGKNCDDLLQSLIERESVF
                     VEKKGNTYFVWTKNNYVSYLTQNNPKFKLLLSNIPKQCAESKNHVENMETLIKNTNQN
                     SDNYNHFKMEFDKCLNESATSIGWASFAQIRQKICESQSLSKEHFYTLASGLVEKHRE
                     NYEISSGGQEGVIMRGLVHGFVRNI"
     gene            7173..8780
                     /locus_tag="VLB45_05835"
     CDS             7173..8780
                     /locus_tag="VLB45_05835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05257.1"
                     /translation="MYAYELESNPYPSSPTPTEKDARVLGGKRHKEAKSAILECIKEL
                     SQKVSCRESNEGDFRVITLIQDVGSGKTHLALHVRNLQNRHSTVCTFLDLSTISPKTM
                     PSLYNAFIKGFENDFYVQLRVKILEYIRDRAEQGDNSAKKALDYTFMDKLTGVTIKQN
                     AEEIISGKKSVSVENLKKFLVQWFNHYESTIIQSIVTNSFDSVGNLEELLGRITAISK
                     LTHRFLGKIVLYEIDEFDGNHDSIEFVKAIINAHLPASILLLISTPSGYAEVQNANPS
                     VFDRLEKANYKIDLAGSNSVEELSEIVLEYIKHNDKNGKFKQKEQEELAEKIRVLNDE
                     FPDFRSVRSIINILYHAMEKSSELQLDKIDEIAIDETIKHAYPGLKVRGSIMDVPISD
                     FIKIRRDCNTESSGPQIKRAISNLVNFAYEMGSIGKLEKQSMPLDVVYQGPYGAKVGI
                     AVVINQNHAKTFEDILNVSKSSAMVDKLVILTNANIPSSDSTTIVNIDKSKMVDLIYF
                     DNKYSDHKIVNSDNEKVQALAKTINII"
     gene            complement(8773..9501)
                     /locus_tag="VLB45_05840"
     CDS             complement(8773..9501)
                     /locus_tag="VLB45_05840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05258.1"
                     /translation="MLNLIFALIILSSTNYAVPGSSLHLYLTDNDLNTSRNGIDTIQT
                     TGLLEFSINGIPITGSAAMKETAVNSGVFAFDVNIPTTINGKSLGSGDVLLIKYHDQT
                     DAAGEPNTITKSISLSKTLSQLSTSTKSVRIGERFLLQLYEPDRNLDSKNPDTISLNQ
                     IEFRAKGIRTTLANSAFDTSTPGLRETGYNTNLFTTNLEIPRHIDGKTIKIGSIVEFR
                     FIDTSSASSTSETLKTKLRVGYSK"
     gene            9575..10843
                     /locus_tag="VLB45_05845"
     CDS             9575..10843
                     /locus_tag="VLB45_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HSD05259.1"
                     /translation="MQEFFLQTGPFNLPVEDFIRDLAIIMILASVVTLAFFKIRQPLI
                     IGYLFAGILIGPLSPLWTSFLPASIEPSSGIEGLGLLSDLTVLNIFADIGIILLLFVI
                     GIEFPIAKIKSIGRVSIGIASIELFLSLGIIFLVSISFGLNFMDSLFIAAALSISSTA
                     IIIKIFEDMGKIKKESSILVLGILIIEDIIAVILIASLQSVVRVGDVSYENIITISAI
                     AALLIIGTLTIGAKIIPRLIDRVASSEHKEILLISVLGLCFGYALLSNLIGLSVGIGA
                     FLAGVLVAESKSAEISKILASPIKDMFAAIFFVSVGALMDISQLQNYVLIAVVLIAVS
                     IVIKCGGTMFGGILFRQGRKKTIRSSLALSPRGEFSIFIVKVGVDMGAVSTFLFPLIG
                     IISIITAFVTPFMMRAGDQLLLKTSKVPEP"
     gene            10840..11262
                     /locus_tag="VLB45_05850"
     CDS             10840..11262
                     /locus_tag="VLB45_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007549863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05260.1"
                     /translation="MTNDELQRLLANVHKDITLFDYDGTKVPCIIFEEKKFDNVMKTV
                     SGKPVSVETNLNILQDGLGHVFVEISLNFSYGGISEKILLYANESIEFFESLAATSML
                     ALSSPHSEYGRSNVFMIQLPKPERAVNALEVIKNGLKK"
BASE COUNT         3684 a         1967 c         2077 g         3603 t
ORIGIN      
        1 actctgaagg catcctataa tgaacttgca aaaatacagg gattaggaga atcgcgagct
       61 cttaagatac gaaaaatgct tgatgcagaa agcaaactcc agaaaaaaat aaatcaaaaa
      121 acattacatg atgtatgaca cacaatccaa atgtctcaca cagactaaac taatgatctc
      181 ttattattgg cttgttgaac atatcaacgg tggttcactt gcggattgga ttgacagaag
      241 actttctctt gtctagttta cgactaagtt tgttaccaca aaggggacat tgtgatgcct
      301 tggaaaatat tttttcacat cctgggcaaa aatacgtcca ttctattaca cgagtaatgc
      361 cttttgtcat gactggaatg acattgatac caagatgttt ggcagtattt gaaactgcaa
      421 aatcatcact aattaattct gcatgcattt gaaggcatag agctattact gaaagatctc
      481 cttgggataa attttgaaag tctccaacat cttttgcttt aagtgaaacg gtttccacat
      541 attctatttc aggatcttta acctctaatc tgttagtatc aataagagca tttactgcat
      601 catgatgttt tttgatgtgt tttatctcat caaaaacaat cgatgttgtg tagcatttct
      661 ctggagaaga aaaaggaact ccagcataaa aggcacttgc atccaaaacc ctagaaacca
      721 agcttactca tctgtcttag accacgtttt tttagcctca caaagactgc aggttttttg
      781 tattttcgta ttgtgatcac atcatcataa cttgctggtt tgactacctg agcatccatg
      841 attatattag aatcatgaga attgatgatc tcaatttttt catccggtac tactagtgat
      901 ggtaatctgt tgactggtgc aactggcgtg atgataagaa catctagact ttcatgtaat
      961 attgggccac caagtgaata ggaatgtcct gtagatccac tcggtgttga tactatgacc
     1021 ccatccattt tttgtgtgac tgtatcattc tgaaacctga tctcaaataa tgaagtcttt
     1081 gtaagattct gtctgtttat gtaaatttca tttaatgctg gaggatattc ttgtccttta
     1141 caggaagcaa caacacgtat tcgtttatca agaaagatat ttcctgaaat tatctgttca
     1201 atggctgtat ctatttggtt caaggttatt tctgaaagta tgcctctatt cccacccaca
     1261 ttgattgcta agagtggaac ttcattttcc aaatatctga atgcccttaa ggttgtacca
     1321 tctccaccaa gggttatggt caggtcgagt tttgcagtct ttaactcctc caaagtactg
     1381 actttttttg caccatctac ttctacagga gaaattgtaa atacctgcgt ttttttacca
     1441 agtaattttt ttgcaacttt ctttgcagca tcctctgact cttttgaacc aactttgcta
     1501 acaacagcga ctctgttaag atccaagcga aaaccagtat tgtaatttca cttaaaaatc
     1561 atctgataag gatgagatat aaatcccctt aacagtaagt gttttaagga aatgagttac
     1621 gccaatccta atgttttaac ggataccgac tgggtttcaa aaaatatttc aaacaaggca
     1681 ataaagattg ttgaagttga ctatgatcct gaaaatgcat acagacaagg ccatattcaa
     1741 caagctagtc tgatatggtg gaaacgtgat atcaacgatc caataacacg tgatattatc
     1801 aacaaaactc aatttgaaga attgatggga agaaatggaa tcctacctga aaacgaggtc
     1861 gtgctgtatg gtgatttcaa taattggttt gcagcttttg tgttttggat cttcaaatat
     1921 tatgggcaca aaaatgtaaa aataatgaat ggcggaagaa agaaatggga actagaaaaa
     1981 aaacaatata caaaagatga gccgcaaatt gcaaaaacaa aatatgtagc acagccacct
     2041 gacgagggtt tgagagcgta tctgtttgat gttcgacgtt ctcttgataa acaagactcc
     2101 attttagttg atgttagatc tgcaaaagag tatactggag aaataacagc tcctccagaa
     2161 tatccgatgg aacacgccca aaggggtgga catataccta gtgcaaaaaa tattccatgg
     2221 gcaacagcag ttaacgatgc tgatggaact ttcaaatcag taaatgaatt aaaacaaatt
     2281 tacgaatcca aaaatgttac accagataag gacgtcatat gttattgcag aataggggaa
     2341 agatcttcgc attcttggtt tgtcctcaaa tatctcttag ggtatccaca agttagaaac
     2401 tatgatggat catggacaga gtggggcaac atgatcggca atcccgttga aaagtaagtt
     2461 gagccaagaa cttcctttgt tgaaacaggg aattttctat ttcatcaggg atggtattca
     2521 agatataatc atggaagaca agacaaaacg aggtcttact gttcaggaaa gatcattgga
     2581 tgaaaattat catgtggaag cagaaaaagg catgatctat gacatggatg gaataggaca
     2641 tcgtgtatgc atacgatggt actttccaaa aaaagactat gatcttggca aggttcttct
     2701 aattgcagaa gaaatggaaa aaaaatacaa aaagctacgt gaagagactt gtccagatta
     2761 ttagatgtta actctagaat caatcaagca aatctatgca tgagcgaaac atcaagtaac
     2821 aatcaggatg ttccaaaaga aaacgtgttt tcattcactc cagtacaact ataaatacat
     2881 attttcaatt ttacatgata gggtatggca ataaacccac taacccagtt tctctttagt
     2941 gtattcatca caattgctgt tattgtcaca ctttacacgt gcaattttta ctatctggta
     3001 tttctctcaa gacaaagaag aaaatctcaa aaaactgatc tgcttggtac tccaattgtt
     3061 acaatacagc ttccaatata caatgaaaga tacgttgcag caagattggt taattctgtt
     3121 tgctcaatgg attatccaaa agaaaaaatg tgtatccaag tattggatga ctctgatgat
     3181 gacacatatg acattttaga aaatctgatt caagattata aaaagaaagg ttttgatatt
     3241 tcacacatca gaagagaaaa cagaaaagga tacaaagcag gagctcttcg taacgcaatg
     3301 aaatttgcca aaggtaattt cattgcaata tttgatgccg atttcatacc gccttcttgg
     3361 tttcttaaaa aagcaattcc atattttgcc caatccgaaa taggactgat ccaatgtaaa
     3421 tggggtcacg taaatgaaaa atattctgca ctcacacagg cacaagcatt aagccttgat
     3481 tttcattttc tcatagaaca aaaggcaaag agcaattccc atctttacat gagctttaac
     3541 ggcactgctg gaatatggag aaaagaatgc atcgaggatg cgggaggatg gcatactgct
     3601 acccttgttg aagatttgga cttgagctac agagcacaaa tgaagggatg gaagtgtctg
     3661 ttcctacctg acattgttgt tgatgcagag cttccagttc aaatgaatgc agcaaaaaga
     3721 cagcagttta ggtgggcaaa gggttccata caatgtgcaa taaaattgat gggagatgta
     3781 gtagtaaaaa aaataccatt tgacacaaag attcaggcct ttgtacagct aacaagacac
     3841 atagtacacc ctcttatgct gattcaattt ttaatacttc caattctgct tcaagcaaaa
     3901 ttcaatcttt atgtaatcag cgtattgcca ttgcttacta tagtagcata tcttgccatg
     3961 gggcctcttg tttacattat gataataaga aacctgtggc agcagagctg gaagtcaaaa
     4021 acattagcgt atttttatct tctgttttat tctgctggta tgtcagtcaa taacacagta
     4081 gcagtatttg acgcgttact tggtaaaaaa aatgagtttc tgcgtactcc aaaatatgga
     4141 attgtcaaaa aaagtgatga ctggagagac aaggcataca atttgccatt tacgaagacc
     4201 acgttacttg agatattctt tggaatctat ggagttttag gaatttttgt tgccattttc
     4261 tcaggcaatc cagtgtttgt acctttgatt gctctccaga ctttgggatt tatctacata
     4321 tcgtatctaa gcgtgtcaca tacgacattt aaaaagaata aatcgcggtc attacacaac
     4381 acatccaagg cagaaaagat ggcaaatgca tactacaaac ttgcaatggt tggaatagtt
     4441 ggatttatcg cgtttggagc attcatggct tattatggat atcagaatga tgtatatcca
     4501 ctggatcaat cacggggtct tttggataga attcaggcca catctgatcc acaaaccata
     4561 attgatgatc ttaaaactat gaagattctc ttgccagagg atggtaaccc agtttggata
     4621 tttcctacag aaaccactga ttttggacga attcaagatg atattgatac tatgatattg
     4681 agcgtagaaa aaatctccac agtcccacaa gatagttctt ctttccatac tggaatgtta
     4741 gatgtacatg aacgagcatt caaaatgaga ttacatctaa tggatgctac accatacatg
     4801 tacgtcagct tttcaaacat tgtttttagc tgtatctgga ttgccgcaat tatgggaata
     4861 tttacgatgt taaaacgaaa aaaggatcgt ctaaaagcct ttgaaatgtc tgacgaagtc
     4921 taaggtattc caagctcggt tcttatttca tcaactgctc ttattccaaa caagtctctg
     4981 acttgctcta ttctcataga ttcttttatc aaatcgcgcc caagttgatt tattagaagt
     5041 ctaaagacat cagtgaatct aatttcccat ctatcagcac agtccaaaat cttgctcaca
     5101 ctccactgtt taacttcttg cggcagcgga attttctttt ctgattccag ggaaagccta
     5161 tcaaaaagat ttaccatatc aaccgtttga gaaatcatct tgtcaagatc atcaagcgtt
     5221 ccatactttg attctgaatg catacgtatg tttgattgaa attcagcaag tcgtaataat
     5281 ccaactacct ctctagatgt attctgggat gttttgtgag ttatattgcg aatttcctgt
     5341 aattcttgat gaatgtcatg agtcctcttg tggaattccg aggtcgccgc tgaattcctg
     5401 tttaataatt cagttaattc agatattttt tcatcaattt gctttgtttt accaagaaca
     5461 ttttctctaa aattagtaaa ttcagttccc acgtttctga tgtctgctcc gaaggtagca
     5521 agtgaatctg tctttcttac aagtgaacca agctcgattc caacgtccct tatgtcggaa
     5581 ctcatgttaa caagagattc tgtttttgat gttatcgcgc caatatcttg cttcaggttt
     5641 gttatgttct gttctaactg agtaatcttc tctgcttttg cagatacatc tttaatctca
     5701 tcacgtattt tttcggtttt atcagagaca tgcattatca tctttgtgtt gtttctaatt
     5761 gagtcaagac tgtttgcaac actttgcatt accttgttaa catctggaag agattcttgt
     5821 ttttcacgaa tcttgtttac ttgatcttga agtctgtctg tttggtcatt gacctttgat
     5881 attaaatttg aatgagcttt aatctggctc aatccagtaa acaatttctg tgtgtcttcc
     5941 tcgagcacat ttatctgtct agcgtttcgc tgtatttgtt ctaatgatga accgacagca
     6001 tctatcatgt tttttaatga aatcagaatt ttttggtttt ctgcaaaaat ctttgacatg
     6061 gatttaactt cgtttgctaa aatctttgta gcatcagaaa tagaattcat tttcttcaag
     6121 acgtctgagc ttgaatctct cactgtcttt ctttttgaat tactggtacg actagaatgt
     6181 tttgccactt tacaataatg caaagataac cgataaaaga gttcggtttt aaatattgca
     6241 tgtattggaa aaaatggcca gaattagttg ttaactagtt ctggatcgtg gagaacctcg
     6301 tggaaagttt tttgaaccaa accagtttct gtttcaataa actgttttgg acaatattcg
     6361 caagttagca tagtatacgg tacaatacgg tactattaat gatcaggtaa ctgtgaaatg
     6421 accttctagg aattttttgg tcagataccc cagagtttct ctcatcactg aatcagtaaa
     6481 ctcgatcatc atcccagaac aagcaggcat tttcagtatt tatgcctaaa gctatctcag
     6541 agaataagaa attttcacta aattgttgtt ccagactacg tggggtctta aataaaattc
     6601 gagaaagatg aacacatgag tagtgaaatt cttgagaagg tagcctctgc aggaatagat
     6661 ggaataaaaa gaattgaact aaaaagaact tttggtaaaa actgtgatga cctcttgcaa
     6721 tctcttatag aaagagagag tgtctttgtt gaaaaaaaag gcaatacgta ctttgtttgg
     6781 actaaaaaca attatgtctc atatctgact caaaacaatc ctaaattcaa actactgctt
     6841 agcaatattc caaaacaatg cgcagaatca aaaaatcatg ttgagaatat ggaaacgcta
     6901 atcaaaaata caaatcaaaa ctctgataat tacaatcact tcaaaatgga atttgataaa
     6961 tgtttgaacg aatctgctac ttctatagga tgggcatcat ttgctcagat tagacaaaaa
     7021 atctgtgaat cacagagctt gtcaaaggaa catttctata cattagcctc tggtttggta
     7081 gaaaaacata gggaaaatta tgaaatatca tctggaggtc aagaaggagt catcatgcgt
     7141 ggactcgttc atggttttgt gaggaatatc taatgtatgc atatgaacta gaaagtaatc
     7201 catatccaag tagcccgact cctacagaaa aggatgctcg tgtgttggga ggaaaaagac
     7261 acaaagaagc caaatctgcg atactagaat gcatcaaaga attatcacaa aaagtttcat
     7321 gcagagaatc taatgaagga gactttagag taattacttt gattcaagat gtaggatctg
     7381 gcaaaaccca tttggctttg cacgtgcgaa atctacaaaa taggcacagc acggtatgca
     7441 catttcttga tctatccaca atatctccaa aaaccatgcc tagtttgtat aatgcattta
     7501 tcaagggatt tgaaaatgat ttttatgttc agctacgagt aaaaattcta gaatacatta
     7561 gggatagagc agaacaagga gataattctg caaaaaaggc tttggattat acgtttatgg
     7621 acaagctcac cggcgtcaca ataaaacaaa atgcagagga aataatatct ggaaagaaat
     7681 ctgtatctgt tgaaaatcta aaaaagtttc ttgtacaatg gttcaatcat tacgaatcca
     7741 caataataca aagtattgtc acaaactcat ttgattctgt aggaaacttg gaagaattgc
     7801 ttggtagaat cacagcaata tcaaaattaa cacatagatt ccttggtaaa attgttcttt
     7861 atgaaataga cgagtttgac ggaaatcatg attcgataga atttgtcaaa gccataatta
     7921 acgcacatct tccagcatca attctattat tgatctctac accttctggc tacgctgaag
     7981 ttcaaaatgc taatccatct gtttttgata ggctggaaaa ggcaaactac aaaattgatc
     8041 ttgcaggttc aaactctgtt gaagagcttt ctgaaattgt tctagaatac ataaaacaca
     8101 acgacaagaa cggaaaattc aaacaaaaag aacaagagga attggctgaa aagattcgag
     8161 ttctcaacga tgaatttcct gactttagga gtgtacgctc tataatcaat attttatatc
     8221 atgcaatgga aaaatcatcg gaattacaat tagataagat tgatgaaatt gctatagatg
     8281 aaacaataaa acacgcatat ccagggctta aggtgcgggg aagcataatg gatgtaccca
     8341 tatctgattt tattaaaatt cgaagagact gtaatactga atcctctggc cctcaaataa
     8401 aaagagctat ttctaatttg gttaactttg catacgagat gggtagcatt ggaaaattag
     8461 aaaagcaaag tatgcctttg gatgttgttt atcaaggtcc atacggagca aaagtgggaa
     8521 tagctgtagt gataaatcaa aatcacgcaa aaacttttga agatatacta aacgtgtcaa
     8581 aatcatcagc tatggtagat aaacttgtga ttcttacaaa tgcaaatatt ccaagctctg
     8641 attctacaac aatagtaaat attgacaaat ctaaaatggt ggatctcata tattttgaca
     8701 acaaatattc tgatcataaa atagtaaatt ctgacaatga aaaggttcag gcacttgcta
     8761 agacaataaa tattatttag aatatcctac tcttaatttc gtttttaatg tttcagatgt
     8821 agaagatgca gacgaggtat ctataaatct aaattccact atagagccaa tctttattgt
     8881 tttcccatct atgtgacgag gaatttctag atttgtcgta aaaagatttg tgttataacc
     8941 agtctccctt aatcctggtg ttgaagtgtc aaaggcagaa tttgctagtg tggttctaat
     9001 acccttggca cgaaattcta tctgattcaa tgatatggtg tcgggatttt tggaatctaa
     9061 attcctgtct ggttcatata gttgtagaag aaacctttca cctattctaa cacttttggt
     9121 ggatgttgac agctgagaaa gcgttttaga aagagaaata gattttgtaa ttgtattagg
     9181 ctctcctgcg gcatctgttt ggtcatgata ttttatcagc aaaacatctc cacttcctag
     9241 agatttacca tttattgttg taggaatatt cacatcgaat gcaaaaacac ctgaattaac
     9301 agcggtctcc ttcattgctg ctgaaccggt tatcggaatt ccatttatgc tgaattccaa
     9361 taatcctgtt gtctgtatgg tgtcgattcc attacgactt gtgttcagat cattatcggt
     9421 taggtacaga tgtagagaac taccaggaac agcataattt gtagaagata aaataattag
     9481 tgcaaaaata agatttaaca taatttcata tggtatttca ataatttatt ttctactaca
     9541 caccattttt aaatgaaaca ttctactcta ttatgtgcaa gagttttttc ttcaaactgg
     9601 accatttaat ctcccagtag aagatttcat acgagatctt gcgataatca tgattttggc
     9661 atctgttgtc actttagctt tttttaaaat acggcaaccg cttattattg gatatctctt
     9721 tgctgggatt ttgataggtc ctttgtcacc actatggact tcatttttac ccgcatcaat
     9781 agaaccatcg tctggaattg aagggcttgg actactctct gatctgacag ttttaaacat
     9841 ctttgcagat attggaataa ttttgttatt gtttgtaatt ggaatagaat ttcctattgc
     9901 aaaaataaaa tctattggac gtgtttcaat tggtatagca tcgattgaac ttttcctttc
     9961 tttaggaata atatttcttg ttagcatttc gtttgggctc aattttatgg attcactttt
    10021 cattgcagct gcattatcga tcagcagtac tgccataata attaaaattt ttgaagatat
    10081 gggaaaaata aaaaaagaat cttcaatctt agttcttgga atattgatta tagaagatat
    10141 tattgcggta attctgattg cttctttgca atcagttgtg cgcgtaggtg atgtttcata
    10201 cgaaaacatc atcacaataa gcgcaattgc agcactactg ataattggaa ctctcacaat
    10261 tggagctaaa ataatcccaa gattgataga tagagtagcc agctctgaac acaaagaaat
    10321 tctacttatt tctgttctgg gtttgtgttt tggatatgcc ttgttatcta atctaattgg
    10381 attgtctgtt ggaataggcg cctttcttgc aggtgttctt gtagctgaat ctaagtcagc
    10441 tgaaatttca aagattcttg ctagtccaat aaaagacatg tttgcggcaa ttttctttgt
    10501 ttctgttggt gcattgatgg acattagtca attacaaaac tatgttttga tagctgttgt
    10561 tttgatagct gtatctattg taataaaatg cggcggaact atgtttggag gtattttatt
    10621 cagacaagga agaaaaaaga ccatccgttc ctcacttgca ctttctccaa ggggtgaatt
    10681 ttcaatcttt atagtcaaag tgggcgttga tatgggagcc gtcagcacct tcttgttccc
    10741 tcttatagga attatatcaa taatcacggc atttgtaact ccgttcatga tgcgggctgg
    10801 agatcaactg cttcttaaaa catcaaaagt gccagaacca tgacaaacga tgagcttcaa
    10861 aggctgctag caaatgtgca caaggacata accctatttg attatgatgg aaccaaagta
    10921 ccatgtatta tttttgaaga aaaaaaattt gataatgtga tgaaaacagt ctctggcaaa
    10981 cctgtctctg tggaaacaaa cctgaacata cttcaagatg gattgggtca tgtatttgta
    11041 gaaatctcac ttaatttttc atatggtgga atcagtgaaa agatcctgct ttatgctaat
    11101 gagtccattg agttctttga atctcttgca gctacttcga tgttagctct ttcttcccct
    11161 cattctgagt atggaagatc caatgtcttc atgattcaac ttccaaaacc cgaaagggct
    11221 gtaaatgcac tagaagttat aaaaaatggc ttaaagaaat aatctatgcg gttgccggga
    11281 tttgaacccg ggtcgcggaa ttggcaatcc cgcatattaa ccaagctgta c
//
LOCUS       DATIDL010000137        42141 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrosopumilaceae archaeon isolate SMAG_U16342
            k141_1985594, whole genome shotgun sequence.
ACCESSION   DATIDL010000137 DATIDL010000000
VERSION     DATIDL010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935836
            Sequence Read Archive: SRR25158326
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosopumilaceae archaeon (soil metagenome)
  ORGANISM  Nitrosopumilaceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrosopumilales;
            Nitrosopumilaceae.
REFERENCE   1  (bases 1 to 42141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/04/2024 05:09:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,520
            CDSs (total)                 :: 1,474
            Genes (coding)               :: 1,474
            CDSs (with protein)          :: 1,474
            Genes (RNA)                  :: 46
            rRNAs                        :: 1 (5S)
            complete rRNAs               :: 1 (5S)
            tRNAs                        :: 43
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42141
                     /organism="Nitrosopumilaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1985594"
                     /isolate="SMAG_U16342"
                     /isolation_source="soil"
                     /db_xref="taxon:2202732"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..886)
                     /locus_tag="VLB45_05855"
     CDS             complement(<1..886)
                     /locus_tag="VLB45_05855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05261.1"
                     /translation="MQELVLVLGSVAGACTAVAIDKIPKRRNDKSIIRANPAIKNQIQ
                     SFKIEKEILTRTISRLYQQESELSKIQRDKLLLRYQNQLGLVIAKIEKLEAASKHPDL
                     GPLGDGLITLMDQKLSQLDQRLHEISSKITVATMTQPQIKQVELPKEIIPEKPIQKKI
                     EIKNEIQKPKEPYTPLPPIEVAIAAPHKSVELITLTEIPNRMSEFPVELVRPVVVTPQ
                     KQIIEEIMTSRTTEPEKPKMDIQPVVETPKIEVRPVIETSKPEPAQIPVEKKIQIPAA
                     VKIPEDEKLEDDDKDIDKI"
     gene            complement(887..1054)
                     /locus_tag="VLB45_05860"
     CDS             complement(887..1054)
                     /locus_tag="VLB45_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L37e"
                     /protein_id="HSD05262.1"
                     /translation="MTKGTTSMGGYTKKHPHIRCRRCGKNSFHARHKRCASCGYPDPR
                     IRKYTWIKWYT"
     gene            complement(1062..1292)
                     /locus_tag="VLB45_05865"
     CDS             complement(1062..1292)
                     /locus_tag="VLB45_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LSm family protein"
                     /protein_id="HSD05263.1"
                     /translation="MSVDMAVKVLDESLNKVVLIKLKGGKVIRGNLHGFDQHMNLLLD
                     SSEEIPSEGESKSLGTIVVRGDNVVIISPPPK"
     gene            complement(1363..2070)
                     /locus_tag="VLB45_05870"
     CDS             complement(1363..2070)
                     /locus_tag="VLB45_05870"
                     /inference="COORDINATES: protein motif:HMM:NF014670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HSD05264.1"
                     /translation="MSEIKHLKDPPLRKLIRNKKQTALIPVGSIEQHGPHLPISTDSD
                     IVSEIAKRISKKCGFLILPTINYGISFEHSPFFNTSISRSTLEKMLVEICVSLSHNKI
                     KNIIILNGHHGNQQALFSVPQKVRHLTKGMVKVKVFSYWHFMKRSFDHAGFVETSLML
                     ALSKKVEMKKTVKGLDVSRLSKKQKEQANKLATKHFITATKNGVWGDPRKATSKDGKK
                     ILSEIVQNFLKESNLPY"
     gene            complement(2067..2924)
                     /gene="purN"
                     /locus_tag="VLB45_05875"
     CDS             complement(2067..2924)
                     /gene="purN"
                     /locus_tag="VLB45_05875"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HSD05265.1"
                     /translation="MEKTSVEITIIGKDREGVVAGFTNFIFQKGGNIEKINQNVVKGL
                     FGMYLEASFTQKINIKKFDSEVRALAKKLKMEVNTHHEATLRKKVAVLVTKEPHCLEA
                     ILEARRKNQLKGEVSVIIGTENTLAPLARKAKIPFAIVNEKEQSTAEEKILEIIKKYN
                     VDLIVLARYMRILSPNFVWRYPNRVINIHPSLLPAFPGAFAYVQAYERGAKIVGCTAH
                     YVTEDLDQGPIIFQDSFAIDANETIESLKKKGQKIEAKTLLEAVKMHLDGRLEVYWGK
                     VHVKSKRSR"
     gene            3035..3265
                     /locus_tag="VLB45_05880"
     CDS             3035..3265
                     /locus_tag="VLB45_05880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05266.1"
                     /translation="MNEEFLEFFNFISNEETLWIDLRAQPKIAVEAIVTIIIIQSKSV
                     LFVLLLEIGIVMNAQKTFVALTLQITKNNNFV"
     gene            complement(3275..3359)
                     /locus_tag="VLB45_05885"
     tRNA            complement(3275..3359)
                     /locus_tag="VLB45_05885"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3321..3323),aa:Leu,seq:tag)
     gene            3410..4759
                     /gene="glmM"
                     /locus_tag="VLB45_05890"
     CDS             3410..4759
                     /gene="glmM"
                     /locus_tag="VLB45_05890"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HSD05267.1"
                     /translation="MAKLFGTNGIRGVFGETFTLDFVSDITLSLATFFKKGPILVGYD
                     GRNSSVIISKIVCSALNSAGLDCATAGLIPTPCLQYVTKSLGYKGGIMITASHNPPEY
                     NGIKPTAHDGVEISRGDELKVEEFYFNKTWKITPEKLGITKQEERAIGTYLDGIKSQI
                     SVEKIRPRKFKIVLDLGNGTQAMTASTLCKDLGCEVITINEKIDGLFPGRGSEPTPEN
                     LHTLSKTVVEYKADLGVAFDGDGDRSIFCDNTGKILTGDRSAILFSKFILAKYPKSKI
                     VTTLNSTSAIEKIATTFNSKVIRTKVGSVEVSREMVSTKALIGFEENGGFMYGKHNQV
                     RDGAMTMAIALDLLANSTKSMSEELRLLQSSFTKKGKIECSKDEAIKIIQTLSNVEHK
                     KDTTDGIKIILDTSNWVMVRPSGTEPIIRIYAESDSEEKLDALFEKYMEKIKSILGR"
     gene            4820..5206
                     /gene="rpl7ae"
                     /locus_tag="VLB45_05895"
     CDS             4820..5206
                     /gene="rpl7ae"
                     /locus_tag="VLB45_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008297732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7Ae"
                     /protein_id="HSD05268.1"
                     /translation="MAKAYYVKFQVPEDLVSPIYEALRVAQQSGKVKKGTNEVTKAIE
                     RGVSKLVVIAEDVEPPEVVAHLPILCEEHGAAYAFVPSRQELGKALGIDVTSAAAAIL
                     DAGDAQHIIDQIVASLSQLKGGQASK"
     gene            5203..5412
                     /locus_tag="VLB45_05900"
     CDS             5203..5412
                     /locus_tag="VLB45_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S28e"
                     /protein_id="HSD05269.1"
                     /translation="MSTTETVVPAEVIQIVGRTGIAGEVIQVRVKIMEGKDKGRILTR
                     NVKGSVRMSDILMLRETEREARKIK"
     gene            5420..5623
                     /locus_tag="VLB45_05905"
     CDS             5420..5623
                     /locus_tag="VLB45_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24e"
                     /protein_id="HSD05270.1"
                     /translation="MSVLVKPCSFCNRPVAKGSGTMLVKNDGTVFWFCSSKCKKNMLV
                     LKRDPRRLKWTNKYVKGGIKVKK"
     gene            5625..6026
                     /gene="ndk"
                     /locus_tag="VLB45_05910"
     CDS             5625..6026
                     /gene="ndk"
                     /locus_tag="VLB45_05910"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007549576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HSD05271.1"
                     /translation="MTEQSLFIVKPDGVERKIVGQILTRFENKGFKIVKLKMFMFTKE
                     MAEEFYSDHKSKPFFGELVSFITSGRVVAAVIEGNNAVATTRLMIGSTKSFEAAPGSI
                     RGDFGLGFTDNIIHASDSKESFEKEVSVVFK"
     gene            6034..7812
                     /gene="infB"
                     /locus_tag="VLB45_05915"
     CDS             6034..7812
                     /gene="infB"
                     /locus_tag="VLB45_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008297744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HSD05272.1"
                     /translation="MQIRQPIVVVLGHVDSGKTSLLDNIRGTGVQGREAGGITQHIGA
                     SFLPPDTIKKVCGPLYEKIGGKDEIPGLLVIDTPGHEIFTNLRARGGSAADIAILVVD
                     VNRGFQPQTNESLKILQSRKVPFVIALNKIDMVSGWKKPKTMFISQSIKEQDPFVQTA
                     LDELLYNVVGTLSILGYNSEAFYRIKDFKKEVSIVPVSARTGEGIPELLAVLVGLTQQ
                     YMQQKLEQKEKSARGIVLEVKDEIGLGVTANIILIDGLLKKGDSIIVAKRDSVVVTKA
                     KALLLPKPLDEMRDPRDKFRPVNEVHAAAGIKIVSPDLEGVLPGSPVYVAENESGIEE
                     FKKLVESEMKSVFIKADTKGVILKCDTIGSLEALTDMLKRQNIPITLADIGPVTRRDV
                     VEALAVKEKDRHLGVVIAFNVKILDDAQEEADSNHVRIFHDQVIYSLIDNYTHWVEED
                     TANEENAIFGELTPVCKFTFLKGYIFRKSNPAVFGARIDVGTLRQKVPIMNADGKKIG
                     ILHQIQDNGKNIDVARKDQEVAVSIQNITIGRQISEEEILYSFPPAPEAKLLLKRFSH
                     KLNPEELQVLHEIIQIQRKLNPMYGY"
     gene            complement(7809..8189)
                     /gene="trxA"
                     /locus_tag="VLB45_05920"
     CDS             complement(7809..8189)
                     /gene="trxA"
                     /locus_tag="VLB45_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HSD05273.1"
                     /translation="MIDKEIDEIMRRKLAEMTSQKEQEIQELTDANFDDVVSSDTPVL
                     VDFWATWCGPCQFMLPIFARLARKFKTIKFARVNVDDAQSVAARYGVYAIPTFLMFKN
                     GQVVDKAVGAVGEPGLNMLAQKYS"
     gene            complement(8231..8647)
                     /locus_tag="VLB45_05925"
     CDS             complement(8231..8647)
                     /locus_tag="VLB45_05925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archease"
                     /protein_id="HSD05274.1"
                     /translation="MVYRYLEHVTDAFIEVTEDSLEEAFASAGMAVVDTVVDIKLVEE
                     KIEKNIMVSGNDLNNLLYNWLEEIIILTITEGFVAKKFSVKVLKNDNYKIIATASGEE
                     INFEKHHFKVEIKSPTLHLMEIKQEKQVMMRFLLDL"
     gene            8758..10038
                     /gene="hisS"
                     /locus_tag="VLB45_05930"
     CDS             8758..10038
                     /gene="hisS"
                     /locus_tag="VLB45_05930"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HSD05275.1"
                     /translation="MNLELPRGMRDITSNETTKIEYIRQKFIETSNLFGFQFMEPSPI
                     ELLSVIETKSGPSIKNEIYYFKDKGDRDVALRFDFTIGLTRYAVSQKSMKLPAKLSAF
                     GGVWRYDEPQKGRYRFFHQWDVEIYGQPNLESDSEIIEFTSKFLSNLKLQNIVIDICD
                     RELVESYVHQIFKSNSSTLVGDMLRAVDKIQKKKRQEIIKEYQEKGYSNVELEKILDF
                     SKIKGTPEEIERNFDVSGLKNWDKLKQLFVSLKNRQVNNVRINFGIVRGLDYYSGIVF
                     EAFDQTSDLGALVGGGRYDTLTKAFGREDLGATGVAGGVERIMFSLEKQGIDVGISTP
                     QVWVAFVNDEMQKVALNLVSQLRQKGIAAEADLIGKPLKKQMEMASNSKFAVIVAPKE
                     YSSNSVVVRNMKDGTEKQILIESILSDPKSNFQF"
     gene            complement(10022..10693)
                     /locus_tag="VLB45_05935"
     CDS             complement(10022..10693)
                     /locus_tag="VLB45_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-6"
                     /protein_id="HSD05276.1"
                     /translation="MGIFKYDVYRSPNVGIYTKVNDSFVFVPNGFATTKAKNLAEFLK
                     TDYIFTSVANTRLLGPLMVINNHGIILPNSVLDDEVAHLKKSTGLNVSVLDTKYTALG
                     NLICVNDKGGIMSPMIPSEFVKAVEDALGIEIIRKRIAKYHQAGAMAVATSRGGIVHP
                     DTEENDIKIMSEVLGVELEAATINGGIPYVASGILANNKSLVVGTLTNGPELVMLTRA
                     FRIEN"
     gene            10769..11221
                     /locus_tag="VLB45_05940"
     CDS             10769..11221
                     /locus_tag="VLB45_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HSD05277.1"
                     /translation="MPIDPDFPKNHTVTGKSKDPLSDNHHLVWGPGKLAEAASDENVK
                     ADYATKGEEIKPIGIHGTFVAVDWDSCIADGICLMSCPVKVFEWYKNPGKTLRRDRLN
                     YTDKANPVKEADCIWCMACVEVCPTKAIKVDQANVEIHNTEIARLSSN"
     gene            11263..11796
                     /locus_tag="VLB45_05945"
     CDS             11263..11796
                     /locus_tag="VLB45_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HSD05278.1"
                     /translation="MPIVDTFPKGYEVIGKIKHADGEHFHFVWGPGRMAEAAEDPACI
                     EAYKAHGEKLEPIGVSGTMVAVDWDSCVGDGACIEACPVQVFQWYRTEKDIPAIKAIN
                     ETFAGTGKTVKEEKLDRSDKADPIREHDCIWCMACVSVCPPLAIKVDQSNLEYHEKAA
                     GTYQTLGSGTANPHAHH"
     gene            complement(11823..12380)
                     /locus_tag="VLB45_05950"
     CDS             complement(11823..12380)
                     /locus_tag="VLB45_05950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05279.1"
                     /translation="MPSFECNEPQFIENIRRLLESESKFVVNRRVQVYDDRKYGPAGL
                     PDETFSQFSSILTRKSQRSSVYAKTHFLDDYHHKVYFLQDLLHSSSKMKYPVLHIPYY
                     KVEYSFRIWGNFYHYIFDVLFKPKIAIEQKRIIKKMNQKIEEDSTMLIHVLQFEPPPE
                     KMLEIDLPKKVLVLDFKKTNNPLEN"
     gene            complement(12367..12639)
                     /locus_tag="VLB45_05955"
     CDS             complement(12367..12639)
                     /locus_tag="VLB45_05955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05280.1"
                     /translation="MSEKKLKSISVFLPEEVVCSSLQKIGFVEQETATTTSDGKFWKK
                     TLYAKGEETISISEELGDYWPKDPIISICGLPSTIKTILGDLDAVL"
     gene            12723..13547
                     /locus_tag="VLB45_05960"
     CDS             12723..13547
                     /locus_tag="VLB45_05960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05281.1"
                     /translation="MKKLVIAILFLLISSSTLPVFAQHHENPPASLGNRIAVMDFVFP
                     ENIAINEDFTLTMILYDETNKKNFEQVSIQLAIYKEGMGKSLMNELFYDKNGEITIDF
                     KHKDFDQPNKREIKAAVEPILGGWMKDYGSNIQILQNIFSENALYTLKATIVTIDHTR
                     TFISEPITFTTEIHLGGHGEVSHTEIDIPDWVRNNAKWWSNGEIDDKTFANGIQYMIK
                     EGIIKIPTTENEQANNEVKIPDWVRNNAKWWSNGEIDDKTFANGIQYLVKIGVIRT"
     gene            13631..13714
                     /locus_tag="VLB45_05965"
     tRNA            13631..13714
                     /locus_tag="VLB45_05965"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13665..13667,aa:Ser,seq:tga)
     gene            complement(13715..14668)
                     /locus_tag="VLB45_05970"
     CDS             complement(13715..14668)
                     /locus_tag="VLB45_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007549586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication factor C small subunit"
                     /protein_id="HSD05282.1"
                     /translation="MTESTMWVEKYRPVKLSSVLNQKEVIGSLKSLLKNVGEIPHLMF
                     SGSAGVGKTTTAMCLAREILGDYWKEYTLELNASDERGINMVRDRVKKFARFSGLDTK
                     IPFKIIILDEADEMTSDAQTALRRIIEDTAKYCRFILIANNISKIIEPLQSRCAVFKF
                     TRISEDDITSHLEEICKKEKIKFDEKGLETIYNYSEGDMRHAINILQATASLGIVNEP
                     NVKSSAGLTKTSDVSGILKLAIAGKISEARNKMIELIKVYGMSESDFLKYLNEETYKM
                     KTSNLDQILEVIAKYDYRLIIGANPEIQLSALLAEIGKIEK"
     gene            14784..15302
                     /locus_tag="VLB45_05975"
     CDS             14784..15302
                     /locus_tag="VLB45_05975"
                     /inference="COORDINATES: protein motif:HMM:NF017704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05283.1"
                     /translation="MLELSDLSQVHTVGYRLQDVKVTFNHDMKIDAAEVSLDAKHGEI
                     LSIPRWVADVLASEKLVDVQDTDMVVALKQALVKENVQGDFDLSTLESDFYIKVRSYM
                     KRLPQQDSDKIESMLNTLMRKRQGKIIKLADSSKLTSDIAKKLSIEERALFDSVHSKS
                     IEFQKQVLGDKK"
     gene            15299..17383
                     /locus_tag="VLB45_05980"
     CDS             15299..17383
                     /locus_tag="VLB45_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minichromosome maintenance protein MCM"
                     /protein_id="HSD05284.1"
                     /translation="MTALSEPVSTPEKRSDQVKEFLTQFKDKSGVYKYVEEIDQMMAK
                     KTQYLVVDYNDLVSHPEIESVFNTDPDEMLSAFAGAIKSILKERFPQYAEKIQYDVRV
                     RIANYPAQRSLREINAEIIGKMTSVSGMVVRSSEIKPLAKELVYLCPEGHQTTVVHEK
                     GLEFNEPLKCSEGRCTHKDLELSPEKSRFIDFQMVRLQELPEDLPPGQLPHYVDVTVL
                     QDLVDNARPGDRIILTGIVRIEQEHIPSMRGKSGIYRLRIQGNNIEFLGGRGTKTSRS
                     TEREEISPDEEKIIKSLGKNTDIYDRLIASFAPHISGQDLIKEAILLLIVGSTQRILA
                     DGAKIRGDINIFLVGDPGTAKSEMLKFCARIAPRGLYTSGRGSTAAGLTAAVVRDKIG
                     IMMLEAGAVVLGDQGLVCIDEFDKMKPEDRSALHEVMEQQSASIAKGGIVATLNARTS
                     ILAAANPMYGKYDPFKNITENVNLPIPLLTRFDLIFVVRDVPSKEKDTRIAKHILNLH
                     RNSVPDTKSLIDVDILTKYLSYAKKIEPVLTKEAEDKILEYYMKMRNVESEGMITVTP
                     RQLEGLVRLSTARARLLMKNQVDAEDAERAIFLIESMLQDAGVDVNTGQVDLGVLQGK
                     PHSEVSKMQLFMDVMKSLEGDEKRPVEEKVFVKELAKTGKFTEDEARKYIKKLQRESA
                     IYESKPGHYNRV"
     gene            17380..19506
                     /locus_tag="VLB45_05985"
     CDS             17380..19506
                     /locus_tag="VLB45_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HSD05285.1"
                     /translation="MKVSQINLPKPAIEFLNSLGYVELYPPQENSVNAGLLEGKSILV
                     SAPTASGKTLIAMLAIMNHLFAKKGKIIYLSPLRALASEKFSEFKKLESLDFERKIKV
                     QISTGDFDIADKNIEKSDVIVLTNEKMDSLIRHGAEWIDDLSLVIADEVHLLGDQDRG
                     PTLEIVLTKLKLLEQKPQILALSATVTNADEIASWIGCELVENNWRPVPLSEGVYDGG
                     RVTMQDGKYFEIETSMRGPSVDLGIDSLKNGGQTILFAETRSRSASLAAKAADVVGKS
                     LPEDVKHDLEAISQEILDDNEHTDLIKNLATLVKKGVAFHHAGLNQKCREIIESEFRS
                     GKIKLLASTPTLAAGVNLPARRVVISNINRYDAKFGGNKPISILEYKQLCGRAGRPQY
                     DAFGEAIIVGNSNSTELIDYYVNGTPEPIVSKLTDDRALRSHILSLISTSPGIKKEEI
                     IDFFSETLAGSQVRKPTIKFKVQITLDYLELEELIKEKGNRFVATEFGKKTSMLYIDP
                     LTAVSFRKSLQKTSKRKNHTFGFLHLITTCEDFFPKFSLRNKDYETIGTLLENHVSEL
                     IESISEYDCSRSLLAMHAWINESSEIFLSDNLGIESGDMHRMVENGDWLVYSLYELAK
                     LEKRDELLDELNTLRQRISYGIKEELIELVKIRGIGRIRSRALYKNGIKSLEDLSKIP
                     IKELAKIDKIGVSVAENIKTQLQKVS"
     gene            19506..20498
                     /locus_tag="VLB45_05990"
     CDS             19506..20498
                     /locus_tag="VLB45_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016939697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine-1-phosphate transferase"
                     /protein_id="HSD05286.1"
                     /translation="MLPQIVAAVSVSIISFFVVYTITPFLIRALEKRNLTVKDYNRVG
                     GAMVARPGGPSLIIGIVISELVLYAFFPTKEIIAILITTSLAFLIGIIDDRKVLGGWF
                     KPVALAGAAIPIILLGSYDSNLAFPLFGSFSIPALYLAIVIFIIPITGNTINSIDVLN
                     GVASGFVTIASFSLTISLLILQNYEIAIASLPLGFVSLAFYKYHKFPSRIFPGDSGAL
                     TLGAMYGVIAIVGHVEIIAAVALLPAVINSFLFLSSVKRIVEHRQIKSNPVTHTDDFK
                     LMATTDKSAPVTLVRLILTSGPMTEKQVGFVIFKLALFSAILAIITTFMMEIKI"
     gene            20558..20731
                     /locus_tag="VLB45_05995"
     CDS             20558..20731
                     /locus_tag="VLB45_05995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05287.1"
                     /translation="MITVTSQISIDGYLTFGSSQLYYASMVKAVVAIVLVIGWIYVLI
                     KIKNWMFNKQIKN"
     gene            complement(20728..21204)
                     /locus_tag="VLB45_06000"
     CDS             complement(20728..21204)
                     /locus_tag="VLB45_06000"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HSD05288.1"
                     /translation="MVTNFISEKAKIGKNVKIWHFSYVGDNTEIGDNVKIGSLAHIDY
                     NVKIGDNTLIEGMVYIPPLSRIGKNVFIGPAAALTNDPYPTSKKMIGVTVEDGAVIGS
                     RAVIKAGVTVGKNSVVAMGAVVTKDVPPNTVVAGVPAKVKYSREEYDKKKKAWENS"
     gene            complement(21213..21482)
                     /locus_tag="VLB45_06005"
     CDS             complement(21213..21482)
                     /locus_tag="VLB45_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HSD05289.1"
                     /translation="MNKKMMEILACPMDKHFPLEIFESDSKDQNVSEGAIYCTKCSRF
                     YPIIEEIPIMLPDELRDKNQDIEFLKKNQSKLPEKITKQASPWHL"
     gene            complement(21479..22414)
                     /locus_tag="VLB45_06010"
     CDS             complement(21479..22414)
                     /locus_tag="VLB45_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010194091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HSD05290.1"
                     /translation="MRVVQIGTGGWGKNHARVLSQLGVLSAVCDADLGRAKEFGTKFS
                     VNHYSSIESLLDSEKFDAAFICTPTSTHFSIATKLLQQKKNVFVEKPMTYLSHEGEEL
                     LKLAQKNKVILTCGYIERFNPAVALVKEYVKSKKYGELIMLEFHRENRMPLHIKDVGI
                     IYDTSVHDIDTAMWLFDDTPEVVFARSGKIRHDHEDFATIMLGFRDSRIAIISSNWIT
                     PTRVRKFNAVCTDAIITSDFISQEVKVETNNGAEIPRKEVEEPLLLEIKNFLASVEGK
                     GELLVKPEHAVNTTKIAEAALLSSQKGTPIYLDLK"
     gene            complement(22417..23334)
                     /locus_tag="VLB45_06015"
     CDS             complement(22417..23334)
                     /locus_tag="VLB45_06015"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016939699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HSD05291.1"
                     /translation="MKSFTAEFAWFDGKFVKSEQAKVSVMTHAIHYGTSVFEGLRGYW
                     NSKNLNIFRLNDHIKRFRNSGKVYSISLKFTDDEISNAIIQLCKKNSVRQSCYIRPFY
                     FVGEYGINLNVNEKTPTHVAIVMFPFGDLFNRNGINAGISSWKRISDNSTPPLAKMGG
                     NYLNSILATQEAKRHGYDEAIMLDHLGNVSEAPGENIFVVRNGKVITPSISSSALEGI
                     TRESVMTLADDFGYEVIEREILRTELYLADEIFLTGTAAEITPVITVDGKKIGTGKVG
                     KITKQMRALYSDITSGRNEKYSDWITGVY"
     gene            complement(23364..23969)
                     /locus_tag="VLB45_06020"
     CDS             complement(23364..23969)
                     /locus_tag="VLB45_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05292.1"
                     /translation="MLLTVAIYLAHLNPVTKAHVEIIEELKKENQVVVMPVVFIKEKE
                     INTRSFPFSFELRRKMIHAIFGDSVSVLSNYTFYAPFIRYLPPFLSPASWKIKKQILS
                     GISDKYFTYTGDKAEGFVLGLYGLNPKVGTRKTISASLVKEKMFEAILGKDTGWENYV
                     PSEVVKIIHENWDVVEKYSNSQDLTYRILGMKFPKHGFWQK"
     gene            24128..24721
                     /locus_tag="VLB45_06025"
     CDS             24128..24721
                     /locus_tag="VLB45_06025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05293.1"
                     /translation="MNLKILSLLVILPLLLMPVVSGYAMPSDKTTMKKQTATMSNMEK
                     MKHTEHTSIQNSYSFLIDETTIKTKRGTVTLSGHGAYSPDEDMISALGKYSIKMGSRI
                     VTGNWKAVGLVDGKGNPYGGSSDDSHVHFIGKTAGLKDPKYTGNNAKRESITRGSHQI
                     WISANAAEGTLCVYGGYVGLSTPRTACVETDTISITK"
     gene            complement(24735..24808)
                     /locus_tag="VLB45_06030"
     tRNA            complement(24735..24808)
                     /locus_tag="VLB45_06030"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(24772..24774),aa:Pro,seq:cgg)
     gene            complement(24862..25701)
                     /locus_tag="VLB45_06035"
     CDS             complement(24862..25701)
                     /locus_tag="VLB45_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008301132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HSD05294.1"
                     /translation="MVVDNKATLTYVQVQKEDLAIFRIVPKDGPVPEYKSGQFITIGM
                     HVPSENKIIRRAYSIASNPENRKYVELYIRWVRKPLPGRLTTQLFSAKEGDEIVWVKP
                     TGLFTLDDKKPNGEKDDRRIVCLGGGTGLAPFVSLAQSLHATGSKRELIVLHGASYVD
                     ELGYRELLTDLENESLDRGKDKWNFRYRAAISRPQEWFNRSWSGQTGRVETFLRTKDG
                     EPSPLEKLVGDKITAENTVFFICGWQGTIDGCMDYLTPKGFVSERNKRPDKSFEIKYE
                     SYG"
     gene            25840..25913
                     /locus_tag="VLB45_06040"
     tRNA            25840..25913
                     /locus_tag="VLB45_06040"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:25874..25876,aa:Ala,seq:ggc)
     gene            complement(26004..26411)
                     /locus_tag="VLB45_06045"
     CDS             complement(26004..26411)
                     /locus_tag="VLB45_06045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05295.1"
                     /translation="MNFLVLSFLIFLGISFGYAYGYGEISASEFKIVNTLGEEINSPV
                     VEQQLNLQTSLRNTSDQKMDWVYIVQIIDKDDAVVDLSFSSGSLLANQTLTTSLSWIP
                     HNYGTYKIETFVWNNLRDIDPLDTKSTFTVTVT"
     gene            26466..26750
                     /locus_tag="VLB45_06050"
     CDS             26466..26750
                     /locus_tag="VLB45_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HSD05296.1"
                     /translation="MSESRETIFIGKKPLMAYVTSTLIQLANQPKISIKARGLSIGRA
                     VDVAQIVARKTENAGYSIGNIKIGSEQLQSQDGRSRNVSTIEIEVKRNTS"
     gene            complement(26782..27627)
                     /locus_tag="VLB45_06055"
     CDS             complement(26782..27627)
                     /locus_tag="VLB45_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016939701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/PEP mutase family protein"
                     /protein_id="HSD05297.1"
                     /translation="MYDSSKPLVVPGVYDAIGAKIAQKAGFEAMFQTGYGTSATLLGM
                     PDYGFIGSTETIENARRICQAVSVPVIVDADTGYGNPLSVWKIVRELESAGASGIFLE
                     DQKWPKRCGHMSGKEVIPKEEYAEKLHAALDARKSKDFIIVARTDARATEGLDAAIER
                     GLRYEKIGADAVFIEAPRSIAEMKKIGRSIDAPLVANMIEGGATPILPAKVLHKIGFK
                     LILYPLSVLFANAFASLQILRELKKTGTTVKFKKNLVEFEQFNDIVDLSKFRKMENKY
                     SRRTK"
     gene            complement(27682..28191)
                     /locus_tag="VLB45_06060"
     CDS             complement(27682..28191)
                     /locus_tag="VLB45_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007549607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HSD05298.1"
                     /translation="MLSQWIQRVGSSVPRGFSRYFILEALKKNPSTGKEIIDAATLQS
                     GGKWKPSPGLIYPLLGRLLDENLIEETKDGKYQITRKGRSMTDDLETLNKIVKNQLDV
                     MFRVGSIGKFVAMDLLERVTSIGNSLSSNVANMTKEETTKYKKFLESEMQKLESAEKK
                     SRGKEIKID"
     gene            28314..30023
                     /locus_tag="VLB45_06065"
     CDS             28314..30023
                     /locus_tag="VLB45_06065"
                     /EC_number="1.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008301060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     flavoprotein subunit"
                     /protein_id="HSD05299.1"
                     /translation="MTDTIEHDLLIVGSGLAGLRAAIQAAKTSSAIKIAVISKLQVMR
                     SHSVSAEGGTAAVLFPEEGDSLESHIYDTVKGSDFLADQDVVERLVHEMPSEIYQLEH
                     WGMPWSRREDGKIGQRKFGGYSFARATYAQDKVGFYEMQTLYDTCQKFDNIEFYNEWF
                     TTSIIHDGQKFCGITAIELSSGTFYTIKGKALIIATGGAGRVYSYSTYALSSTPDGLD
                     MAYRAGMALKDMEFVQFHPTGILPSGILITEGARGEGGYLLNKDGERFMKRYAPGLME
                     LAPRDIVSRSMMTEMKEGRGFKNETGVDCLKLDLRHLGDDIIKEKLAAIREISMKFSG
                     IDPSKEPIDVRPVAHYMMGGIHTDIDGATEIQGIWAAGEAACNSVHGANRLGANSTSE
                     CLVWGKVTGELAAKYVLDAKMVPFPLHLVAAEEKRIYDGIFRGRGDVNPYEVKQELTD
                     TMNEKAYVFRTEKDMIDGLKKLRELKERTWKHVDDKASEYNTNFTNVMELDSMFRVAE
                     VILVGAIKRKESRGSHARIDYPARDDKNFLHHTLAYYDTKEPILKEHPVTITKYKPVE
                     RKY"
     gene            30024..30455
                     /locus_tag="VLB45_06070"
     CDS             30024..30455
                     /locus_tag="VLB45_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase"
                     /protein_id="HSD05300.1"
                     /translation="MEKQSNREGIKGWLNPGRFGLERVAYWLMRLTGLGLLVYLIAHV
                     FETSSIVNGKEAWEKMLALTQTTEGHIILAIVIGMCVFHTANGIRVMLGHGGVGVGKP
                     GRPDYPYKAASLNSRHKLCILASIGLAALAMMYGLAVLFGE"
     gene            30458..30802
                     /locus_tag="VLB45_06075"
     CDS             30458..30802
                     /locus_tag="VLB45_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase"
                     /protein_id="HSD05301.1"
                     /translation="MQESVIMKIHYGTALGAVALVAIHILFRMTQNFSDSLQYESVIA
                     NYQFLPYAIMLELVLILLSVHGFNGLRVILLELKQGVGYEKAVNYGCLGAMATLIAYG
                     SRTILMTGFGMT"
     gene            30804..31574
                     /locus_tag="VLB45_06080"
     CDS             30804..31574
                     /locus_tag="VLB45_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HSD05302.1"
                     /translation="MAAITPRILEEVSSTAVSPSNTITLRISKHNPKSDSSTKFVEFK
                     VPVQKWTTVLDTILAVKQHLDPSVSVRYSCRQASCGSCGMKINGKPALACYTKISELN
                     SNIVTVEPMNNFPIIRDLVVDFTQLFSNHKKIKPYIINEDSEIKSDTKEFLQTPKDVE
                     EYLQFSYCIKCGLCNSACPTMATDSSFIGPQALAQAYRYVADNRDNGKKDRLKIVDDS
                     HGIWRCHFAGSCSQVCPKGVDPAMGIQMLRGYMLGFRK"
     gene            complement(31578..31898)
                     /locus_tag="VLB45_06085"
     CDS             complement(31578..31898)
                     /locus_tag="VLB45_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007549614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein"
                     /protein_id="HSD05303.1"
                     /translation="MAQETQQLRRKIFDELTKIVDPEINVSIMELELIDNIDISGSAV
                     KVDLHLTSPFCPAIFGFKIAQDVHDNLLKLDGVDDVKLNVSNHFMAEAINNQVNASKN
                     PKKP"
     gene            31949..33409
                     /gene="argH"
                     /locus_tag="VLB45_06090"
     CDS             31949..33409
                     /gene="argH"
                     /locus_tag="VLB45_06090"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HSD05304.1"
                     /translation="MYRSRFDGSLDEHTLNYLSSISDDVEIAIYDIVGSQAHVIMLLE
                     NKIITKHEAVKILSVLEKLKKENLSIKSDSEDIHELIESLVIKKAGKQAGGKMHTARS
                     RNDQVSLDIRMKMRDDINIICACLLDTIETLVSLAEKNGDAVMPLYTHLQQAQIGVFS
                     HYLLAYADALFRDVDRFYVTYGRVNESPLGAGPVGGTSLPINRQSTAKMLGFKGLIEN
                     SIDATGARDFVAEYVGHVAILMTNLSRLAEDFILWSTSEFSFIELSDKFASTSSVMPQ
                     KKNPDILELTRGKTAQVIGYQMAILSNMKGLPSGYSRDLQQIKTSIWASSKIAISTLL
                     VINAMLKTMKVNRQKMKKSIDSGYSISLDIAEHLVKKGVPFRTAHKIVGSLVQTASNS
                     KKALSGLSLSEIRSSLKESDVKPKELYEIMQSMTAHSSLSTRTSQGSAGKSEQLRMVS
                     DRKRKVHAYRGGTVKRTNEIKTAFENLSSKIKIILK"
     gene            33549..33758
                     /locus_tag="VLB45_06095"
     CDS             33549..33758
                     /locus_tag="VLB45_06095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05305.1"
                     /translation="MFQFIHQQSEKPNYTAGQTWGALKKAWRGYKIAKVQSDSSKMTE
                     YAKKIRTLQHDLGVKQAEFPELNLS"
     gene            complement(33755..35059)
                     /locus_tag="VLB45_06100"
     CDS             complement(33755..35059)
                     /locus_tag="VLB45_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CFI-box-CTERM domain-containing protein"
                     /protein_id="HSD05306.1"
                     /translation="MIQIASAQTNSEQIGPQSFDFEISTDQPIYHYTYEQSKHEEIRI
                     LAKIPQIIPNEKVVVESIYSRGPHRYTDTYSVCSDITATNCFAKLEGDIYFVESNRQY
                     GNLFGKWALDATYAGKRSTTIFEITTLNKFDIDVARQEYTIKDLKDIGLEIIFSGSEI
                     SDQTTLTLEIFKINPTGQELVLKRDIPVQVDSEPPYFNRLPITFPNNVPFDAGQYNIT
                     ARWGHLYDSDIFEIVKPVLTTNDPIEDTNGVKNGGCLIATAAFGSELAPQVQMLREIR
                     DEKFLSTISGASFLQAFNSLYYSFSPTIANAERQNPALQVVIKTALYPLIGILKTSEI
                     TTMLGGEIGIISTGFVSGTLIGAVYLWPLSLRFDITREYRKVILVMITSIGLIVFGTV
                     FHNTNVLMVSTIIFVLTTFTFGVLLARSIINLVRPKNKKRAN"
     gene            complement(35133..35206)
                     /locus_tag="VLB45_06105"
     tRNA            complement(35133..35206)
                     /locus_tag="VLB45_06105"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(35170..35172),aa:Lys,seq:ttt)
     gene            35306..35415
                     /locus_tag="VLB45_06110"
     tRNA            join(35306..35320,35353..35415)
                     /locus_tag="VLB45_06110"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35374..35376,aa:Asp,seq:gtc)
     gene            complement(35472..36281)
                     /locus_tag="VLB45_06115"
     CDS             complement(35472..36281)
                     /locus_tag="VLB45_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010194121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HSD05307.1"
                     /translation="MQVIEILQEASMRVYNNVRHLAGTKESGGDFGRGAGGDISRNID
                     IIAEKTVLDYLKEIKFDCIVLGEECGRVVLSENPKGYVIMDAVDGSTNAVRGIPFFCC
                     SLAFATENKLSTVTDGVVTDLFRGDMYSATKGKGAFLNGNRISVNKDKKPIYFVVGLD
                     ISGISPVTLERLSPIITTSNHIRHFGANALEFGMFAKGLMDAFIDLRGKIRVTDIAAG
                     RLIALEAGGYVVDQNGKPLDSDLSYETKLSFIAAASKEILDDMVKRLKIGN"
     gene            36368..37486
                     /locus_tag="VLB45_06120"
     CDS             36368..37486
                     /locus_tag="VLB45_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008301045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05308.1"
                     /translation="MSGKDESVFSKDALLSTKPGKEIVKQALFKSKGYKQFDKYKKQA
                     EEEFSNFVNRFTDDLLKEIKSDPSPDVTQQNFAEEIGSNEIILEKSQTNQIKSKLENY
                     DILKDRVSRILNSNFVKMTFPVFSALYDASSEYFGDKQDIQLRNDIIDGHIIAIDLSE
                     PMDRIMDRDEDLEYLDDYKLMNPYLLKLARDKIAKGGKEVLEEFERGFKDARIGQYLD
                     FKLKTKPTNISEEDMMQCYKKYRAVMGTAGRNMALSRKPLAEIFYLGMAKAAESVGCG
                     NEIEDSIKNKFVKIPSWPLYYSLLTNDVKKGFEITMKKSNLYLDEARLALELLPENFS
                     HRNFLDFLFLTVEHYNLFWYNQLQKENLWSEFSAKLPK"
     gene            37492..38652
                     /locus_tag="VLB45_06125"
     CDS             37492..38652
                     /locus_tag="VLB45_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008301074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HSD05309.1"
                     /translation="MMWSEKYRPKNFLDVIGNEEAKVSIIEWLGKWVKGSKPILLVGP
                     PGIGKTTLALLCAKQFGYDLISLNASDVRSKQKIQDVLLPVLGHTSVLGKPMIFIDEV
                     DGIHGRADYGGVEALVDLLKEPTVPIVLAANYERKDKMKNIKKVTKMIQLKPIPPRLL
                     RLYLESILKKEGAKLGVGTILRLVVESRGDIRSIINSAQALVSGFEPQTEKSFVEMDV
                     EEAVNAFFKAKSVDEARIILYSLRIDPQEKIQAFYSSVITSSLSKTDMQKMLQVISEA
                     DLLYGKIRKTQEWRLLRYMDSILLGLYKEGTAARYSQYNLPWPLLNRLRWDGKAIKGM
                     ISILAKKMHVSTSDFATFYFPYMLFCMKNKFLDLNFDESYNEILQKEMAALK"
     gene            38649..38966
                     /locus_tag="VLB45_06130"
     CDS             38649..38966
                     /locus_tag="VLB45_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05310.1"
                     /translation="MSWRKMPMKFSGMCVVCKKKIEINEVGLWAKGLGVKHLTCAEVL
                     ELRCVICGGPAGCPQCEFRDDCDLSKVSQLCICKKCNDPSDSFVSYQKAIGKKFPILN
                     LKI"
     gene            39306..40853
                     /locus_tag="VLB45_06135"
     CDS             39306..40853
                     /locus_tag="VLB45_06135"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HSD05311.1"
                     /translation="MHTDKIKNFLQKRRTANQGGGQDKILSQHEKGKLTARERIDLLL
                     DEGSFTEIDALTTHHYHEFEMQDKKFFGDGIIAGYGTINGRQVFVFAYDFTILGGTLS
                     QMGAKKITKVMDHAVRVGCPIIGIIDSGGARIQEGILSLDGFADIFYHNQLASGVIPQ
                     ITASIGPSAGGAVYSPAMTDFVIMVDKLATMFVTGPEVVKTVLGEEVSFDELGGAMSH
                     GKNSGVAHFVAKNEYQCMDYVKTLLSYIPQNNTEEPPIVSTGDDPNRLDNNLINIVPE
                     NPLQPYDMKEIIFSILDNHTFFEVHELFAPNVIIGFGRMNGRTIGIVANQPMVLAGAL
                     DIDSSNKASRFIRFCDCYNIPIVTFVDTPGYMPGTNQEHNGIIRHGSKLLYAYCEATV
                     PKITIIIGKAYGGAYIAMGSKNLRTDMNYAWPTAQIAVLGSEAAVKIMNRKEIANAKD
                     PEAMKKQLVDNFTEKFANPYVAASHGTIDTVIDPAETRPMIIKALEALVNKREKQLPR
                     KHGNINL"
     gene            40859..>42141
                     /locus_tag="VLB45_06140"
     CDS             40859..>42141
                     /locus_tag="VLB45_06140"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HSD05312.1"
                     /translation="MIEKILIANRGEIAIRVIKACKTFGLKSVAVYSDEDVNSRHVKM
                     ASEAYHIGSAAPSQSYLNMEKIIEVALNSGADAIHPGYGFLSENADFADLCEKRKLNF
                     IGPSGKSMRLCGDKMLCKDAMAKAKVPTVPGSPGIVEEVEKALDIAHQIGYPVLLKSV
                     FGGGGRGIRLVHNEKELREAFEIASGESKAAFGKSALFVEKFLPKIRHIEYQLFRDKH
                     GNAVHIFERECSIQRRHQKLIEMSPSPVVDQKTRDEIGKLAINAAIAVDYTNAGTAEF
                     LRLDDGTFYFIEINARLQVEHPVTELVSGLDLVKLQIDIANGKEIPFKQKDLKLKGCA
                     IECRINAEDTFLDFAPSTGPIPDVNIPSGPGVRVDTYLYPGCTVSPYYDSLMAKLVTW
                     GGTFEESRQRMQLALSDFYIEGVETSIPLYKTILD"
BASE COUNT        13761 a         7529 c         7801 g        13050 t
ORIGIN      
        1 ttattttatc aatgtctttg tcatcatctt ctaacttttc atcttccggt attttgacag
       61 ccgctggaat ctggatcttt ttctcaacag gaatctgtgc aggttctggt tttgaagttt
      121 ctatgactgg acgaacctca attttgggtg tttctacgac tggttgaata tccattttgg
      181 gtttttcagg ttctgtagtt ctacttgtca tgatttcttc tattatttgc ttttgtggcg
      241 taacaactac tggtctgaca agctcaacag gaaattctga catcctattt ggtatttctg
      301 taagtgtgat gagctcaact gatttatgag gtgctgcaat ggcaacttca attggtggca
      361 ggggggtgta gggttcttta ggtttttgaa tctcattttt aatttctatt ttcttttgga
      421 ttggtttctc aggaatgatt tcttttggta gttcaacttg ttttatttga ggctgtgtca
      481 ttgtagccac tgttatcttg gaagaaattt catgtagtct ctgatccagt tgtgaaagtt
      541 tttgatccat taatgtgatc aatccatctc ctagagggcc aagatctggg tgtttgcttg
      601 ccgcttctag tttttcaatt tttgctatta caagtccaag ttgattttga tatctaagta
      661 aaagcttatc acgttgaatc tttgaaagct cagattcttg ttgatataat ctagatattg
      721 ttcttgttag gatctctttt tctattttga atgattgtat ttgattttta attgctgggt
      781 ttgccctaat gatagattta tcgtttcttc gttttggaat tttatctatc gcaactgcgg
      841 tacaagctcc tgcaactgaa cccagaacaa gaactaattc ttgcatctat gtgtaccatt
      901 tgatccaggt gtattttctt attctaggat ctggatatcc acaacttgca caacgtttgt
      961 gtcttgcatg gaatgagttt tttccacatc tcctacatct tatgtgaggg tgtttttttg
     1021 tatacccgcc cattgaggtt gtacctttag tcataataca atcactttgg aggtggagat
     1081 atgattacca cattgtctcc tctgacaact atggttccta gactttttga ttcgccctca
     1141 gaagggatct cttctgatga gtcgagtagc aggttcatgt gttggtcaaa accatgtaga
     1201 ttgcctctga tcactttgcc gccttttaat ttgataagga cgactttgtt gagactctca
     1261 tccagtactt ttactgccat atctacggac atttatttca cttattacac cctctatcga
     1321 gggtatatat taaaatttca ttggtgtaat tttggggtta tgtcagtaag gcaagtttga
     1381 ctcttttagg aaattttgta caatttcaga caaaattttc ttgccgtctt tggatgttgc
     1441 ttttctagga tctccccaca ccccattctt tgtagctgtg ataaagtgtt ttgtagcaag
     1501 cttgtttgct tgctcttttt gttttttaga tagtctagaa acatccaatc cttttacagt
     1561 ctttttcatt tcaacttttt ttgatagtgc taacataagc gatgtttcta caaatccagc
     1621 atgatcaaag ctccttttca taaaatgcca ataggaaaac acttttactt ttaccatgcc
     1681 ctttgtaaga tgacgtacct tttgtggaac actaaacaat gcttgttgat ttccatggtg
     1741 tccatttaga attataatat tttttatttt attgtgtgat aaagaaacac atatctcaac
     1801 tagcattttt tctagagtag atcgggaaat acttgtatta aaaaaaggag aatgttcgaa
     1861 agaaattcca tagtttattg taggtaatat caaaaaccca cactttttcg aaattctctt
     1921 tgcaatctct gatacaatat ctgaatctgt agaaatggga aggtgtggtc cgtgttgttc
     1981 gattgaacct acaggaatta acgcagtttg tttcttattt ctgattaatt ttctcagagg
     2041 ggggtccttt agatgtttta tctcactcat ctggatcgct ttgatttgac atgaaccttt
     2101 ccccaatata cttccaatct tccatcaaga tgcattttta ctgcttctag caaagttttg
     2161 gcttctattt tttgaccttt cttttttaat gattcaatag tttcgtttgc gtctattgca
     2221 aatgaatctt gaaaaattat cggtccttga tccaaatctt ctgttacata atgtgcagta
     2281 catccaacta tctttgcacc tcgctcatat gcttgcacat atgcaaaagc tcctggaaat
     2341 gcaggtaaaa gtgatggatg gatattgatt actctgtttg gatatctcca gacaaaattt
     2401 ggtgacaaaa tacgcatgta tctggctaaa actatcaaat ccacattgta tttttttatt
     2461 atttcaagta tcttctcctc tgcagttgac tgttcttttt catttacaat agcaaaagga
     2521 atctttgcct ttcttgccaa tggcgcaagt gtattttctg tgcctattat cacactaact
     2581 tcgcccttta gctgattttt tctcctagct tctaaaatgg cttccaaaca atgtggttct
     2641 tttgtaacta acactgctac tttttttctt aatgtagctt catggtgtgt atttacttcc
     2701 attttcaatt tttttgctaa tgctctgact tcgctgtcaa attttttgat gttgattttc
     2761 tgtgtaaatg aagcttcaag atacattcca aacaaaccct tgactacgtt ttgatttatt
     2821 ttttcaatgt ttccaccttt ttgaaatatg aaattagtaa aaccagctac taccccttcg
     2881 cgatcttttc ctattatggt aatctctaca cttgtttttt ccattctaga ttgattggca
     2941 gatcgtactt tattaatgct taaataacca tatggaatat ctcgtagtta gataatgtgt
     3001 gtatatgata tacatccaat tttataatgc gtgagtgaat gaagagtttc ttgagttttt
     3061 caatttcata tcaaatgagg aaacattatg gatagatcta agggcacaac caaaaatcgc
     3121 agtagaagca atagttacga tcataataat ccaatcaaag tctgttctgt ttgttctact
     3181 attggagatt ggcattgtta tgaatgctca aaaaactttt gtagcgctca ctttgcaaat
     3241 cacaaagaac aacaactttg tataataaaa taagtgcggg aggtgggatt tgaacccacg
     3301 aactcctaaa agacagggtc ctaagccctg cgcctttgac caggcttggc aactcccgca
     3361 tgcaccatgc cttttaagac aaatttatct attattgatg gactagcaga tggcaaaact
     3421 gtttggaact aatggaatta gaggtgtatt tggagaaact tttacactag atttcgtatc
     3481 tgatatcaca ttatctcttg caactttctt caaaaaagga ccaattcttg tgggatatga
     3541 tggaagaaat tctagcgtaa taatttccaa aatcgtatgc tctgccttga attctgctgg
     3601 tcttgactgt gcaacagcgg gattgattcc aaccccttgt ttgcagtacg ttaccaaaag
     3661 tttaggttac aaaggtggca taatgattac tgcatcgcat aaccctccag agtataatgg
     3721 gataaaacct actgcgcatg atggtgtaga aatttcaagg ggagatgaac taaaagttga
     3781 agaattttat tttaacaaaa cttggaaaat tactccagag aagctaggca taaccaagca
     3841 agaagaaaga gctattggaa cctacctaga tggtataaaa tcacaaatca gtgttgaaaa
     3901 aatcaggcct agaaaattca agatagtgct agatctgggt aatgggactc aagccatgac
     3961 cgcatctact ttgtgtaagg atcttgggtg tgaagtaatc acaattaacg aaaaaattga
     4021 cggattattt ccaggaagag gctctgaacc aactccggaa aacctccaca ctttatcaaa
     4081 aactgttgtt gaatacaagg cagatctagg ggttgccttt gacggtgatg gtgataggag
     4141 tatcttctgt gataacactg gaaaaatttt aactggtgat agatctgcta tacttttttc
     4201 aaaattcatt ctagcaaagt atcctaaatc aaaaatagtc actactttaa actctacatc
     4261 tgcaattgaa aaaattgcaa ccactttcaa ttcaaaagta attagaacaa aagttggaag
     4321 cgtagaagtt tcacgtgaaa tggtatcgac aaaagcacta attggatttg aagaaaatgg
     4381 aggattcatg tatggaaaac ataaccaagt acgtgatggt gctatgacta tggcaatagc
     4441 attggactta cttgccaatt ctacaaaatc aatgtcagaa gaactcagac ttttacaatc
     4501 atcgtttaca aaaaaaggta aaatagaatg ctctaaagat gaggcaataa aaataatcca
     4561 aactctaagt aatgttgaac acaaaaaaga cacaactgat ggtatcaaaa ttatacttga
     4621 tacgtcaaat tgggtaatgg taagaccaag cggaactgaa ccgataatca gaatctacgc
     4681 tgagtctgac tcggaagaaa aacttgatgc tctttttgaa aaatacatgg aaaaaataaa
     4741 atcaattctt ggcagataac acttttaaaa ccaattggaa gcacactaag gatggaaatg
     4801 atccctttgg gtgatacatg tggctaaggc ctattatgta aaatttcaag taccggaaga
     4861 cttggtcagt ccaatctatg aagcactaag agttgctcaa cagagtggaa aagtaaagaa
     4921 aggcaccaat gaagtcacaa aagcaataga gcgaggagta agtaagcttg tggtaattgc
     4981 tgaggatgta gaacctcctg aagtagtggc acaccttcca atactttgcg aggaacatgg
     5041 tgcagcatat gcatttgttc caagccgaca agaactaggt aaggcgcttg gaatcgatgt
     5101 tacttctgca gcggctgcaa tcttagatgc aggagatgca caacatatca ttgatcaaat
     5161 agtagcatca ctttcacaac taaaaggtgg gcaagctagc aaatgagtac cactgaaaca
     5221 gtagtcccag ctgaagttat ccaaattgta ggtaggacag gaattgccgg tgaagtaatt
     5281 caagttagag taaagataat ggaaggaaaa gacaaaggaa gaattctgac aagaaacgtt
     5341 aagggttcag tacgaatgtc tgacattttg atgttaagag aaacagaacg tgaagcaaga
     5401 aagataaagt gattataaat tgagcgttct agtaaaacca tgcagttttt gtaatcgtcc
     5461 agtagcaaaa ggatctggga caatgcttgt caaaaacgat ggaactgttt tttggttttg
     5521 ttcgtcaaaa tgcaaaaaaa acatgttagt actaaagcgt gacccaagaa gactaaagtg
     5581 gacaaacaag tacgtaaaag gcggaataaa ggtcaaaaag taaaatgact gagcagtcgc
     5641 ttttcatagt aaagcctgac ggtgtagaaa ggaaaattgt cggacaaatt cttacaagat
     5701 ttgaaaacaa aggattcaaa atagttaaac taaaaatgtt tatgttcaca aaagaaatgg
     5761 cagaagaatt ctattctgat cataagtcaa agccattttt tggtgaattg gtatctttca
     5821 ttacgtctgg tagggttgtt gcagccgtaa tagaaggcaa taatgcggta gctaccacaa
     5881 gactcatgat cggttctaca aaatcttttg aggctgcccc aggttcaata cgaggagatt
     5941 ttggcctcgg atttacagat aacataattc acgcctctga ttctaaggaa agttttgaaa
     6001 aggaagtatc tgtagtattc aagtgaaaag aggttgcaaa tacgacagcc cattgtagta
     6061 gttctaggtc atgttgattc tggaaagacc tcgctacttg ataatattcg tggaactggt
     6121 gtacaaggca gagaagctgg cggaattaca cagcacatag gtgcaagctt tcttccacca
     6181 gatacaatca aaaaagtttg tggtccgcta tatgaaaaaa taggtggaaa ggacgagatt
     6241 cctggtctgc tggtgattga tactcctgga catgaaatat ttacaaacct acgagcacgt
     6301 ggaggttctg ctgcagacat tgcaatttta gtagttgatg tgaaccgagg atttcaaccg
     6361 cagaccaatg aaagcttgaa aatattgcaa agcaggaaag tcccgtttgt aatagcacta
     6421 aacaaaatag atatggtctc tggctggaaa aaacccaaaa ccatgttcat ctctcaatca
     6481 ataaaagaac aggatccgtt tgtacaaact gcacttgacg aattacttta caacgttgtc
     6541 gggacattat ctattctcgg ctataactcc gaagcatttt acagaataaa agatttcaaa
     6601 aaagaagttt ctatagtccc tgtaagtgct agaactggag aaggaattcc agaattattg
     6661 gctgtgttag ttggtcttac acagcaatac atgcaacaaa aactagaaca aaaagaaaaa
     6721 tctgcaagag gaatagtgct tgaagttaaa gatgaaattg gactgggtgt aactgcaaac
     6781 atcattctga ttgatggact gctcaaaaaa ggagattcta taatagttgc aaaacgagat
     6841 tccgtggttg ttactaaagc aaaggcacta cttttaccaa aaccacttga tgagatgaga
     6901 gatccgcgtg acaagttcag acctgttaat gaagtacatg cagcagctgg catcaaaatc
     6961 gtctcgcctg atctagaggg agtattacct ggaagcccag tatatgtagc agaaaatgaa
     7021 tctggaattg aagaattcaa aaaactagtt gaatctgaaa tgaaatctgt ttttatcaag
     7081 gctgatacaa agggtgtcat actcaaatgt gatacaatag gttctcttga agcactaact
     7141 gacatgctaa aacgccaaaa tatccctatt accttggccg atattggacc tgttacacga
     7201 cgtgacgttg ttgaagcact agcagtcaaa gaaaaagaca gacatctagg agttgtaatt
     7261 gcatttaatg tgaaaattct tgatgatgca caagaagaag ctgactctaa tcatgtaaga
     7321 atatttcatg atcaggtaat ctatagtcta attgacaatt acacgcactg ggtagaggaa
     7381 gacactgcaa acgaagaaaa tgcaatattt ggagaactca caccagtatg caagtttact
     7441 tttcttaaag gatacatatt tagaaaaagt aaccctgcag tttttggtgc ccgcattgat
     7501 gttggtacac tacgacaaaa ggttcctata atgaatgcag atggtaaaaa aattggcata
     7561 ttgcatcaaa ttcaggataa tggaaaaaac atcgatgtgg caagaaaaga tcaagaagtg
     7621 gctgtttcaa ttcaaaacat aacaataggt agacaaatct cagaagaaga aattctgtat
     7681 tcttttcctc cagcaccaga ggcaaagctt ctactcaaaa gattctcaca taaactaaac
     7741 cctgaagaat tgcaagtatt acatgagatt atacaaatac aaagaaagct aaatccgatg
     7801 tacggttact aggaatattt ctgagcaagc atattaaggc caggttcccc caccgcaccc
     7861 actgccttgt ctactacttg accattcttg aacattagaa acgttggaat tgcatatact
     7921 ccatatcttg cagcaacact ttgtgcatca tcaacattta cgcgagcaaa ttttatcgtc
     7981 ttgaatttcc ttgcaagtct tgcaaatatc ggaagcataa attggcacgg accacaccac
     8041 gtagcccaga aatccactaa aacaggtgta tcactagaca caacatcatc aaaattcgca
     8101 tcagttagtt cttgtatctc ttgttctttc tggctagtca tctcagctaa ttttcttcgc
     8161 attatctcgt cgatttcttt atcgatcatg tggagatcaa tgtagaaata attgatattt
     8221 aataatttgt ttacaaatca agaagaaatc tcatcataac ttgcttttct tgttttatct
     8281 ccatcaggtg aagtgttggt gacttgatct ctaccttgaa atgatgtttc tcaaaattga
     8341 tttcctctcc agaggcggta gctataattt tgtaattgtc gttttttaac actttcaccg
     8401 agaatttttt tgctacaaac ccctcagtta ttgtaagtat gattatttct tcaagccagt
     8461 tgtaaagtaa attgttcaga tcattgccgc taaccatgat gtttttttct attttttcct
     8521 caactaattt gatatctacc accgtatcaa caactgccat acctgcactt gcaaaagctt
     8581 cttcaagact gtcttccgta acctctatga acgcatctgt tacgtgttct agatatcgat
     8641 acaccattga acagctcttg tggctgtctt ctattaattc attgtaaagt cacatgttat
     8701 tattacaacg actatagaga tcagtaaggt ctaaatgcat tggaactaga gtgatttttg
     8761 aatttggaac taccacgtgg aatgagagat atcacatcta acgaaactac aaaaatagaa
     8821 tatatcaggc agaaattcat tgaaacctca aatcttttcg gattccaatt catggaacct
     8881 tctccaattg aattactttc tgtaatagaa acaaaaagtg ggccatcaat aaaaaatgaa
     8941 atttattatt ttaaagacaa gggagacaga gatgtcgctt taaggtttga tttcactata
     9001 ggcctcacta gatatgcagt ttcacaaaaa tcaatgaaac tgccagcaaa actatctgca
     9061 tttggaggtg tctggcgata tgatgagcca caaaaaggaa gatacagatt ttttcaccaa
     9121 tgggatgtag aaatctatgg tcaaccaaat ttagaatctg attccgaaat aattgagttt
     9181 acatcaaagt ttctttctaa tctaaaattg caaaacattg taatagacat atgtgatcga
     9241 gaacttgtag aatcatacgt acatcaaatc ttcaagtcca attcatccac tctagttgga
     9301 gatatgctca gagcagtaga taagattcaa aaaaagaagc ggcaagaaat cataaaagaa
     9361 tatcaagaaa aaggctactc aaatgttgag cttgaaaaga tcctggattt ctctaaaatc
     9421 aagggcactc ctgaagagat agaaagaaat tttgatgtaa gtggtctaaa aaattgggac
     9481 aaattaaaac aactgtttgt ctctctaaaa aacagacaag ttaacaacgt tagaataaac
     9541 tttggaattg taaggggtct tgattactat tctggtattg tatttgaggc ctttgatcaa
     9601 acctctgatc ttggtgcatt agtaggtgga ggaagatatg acacactaac aaaggcattt
     9661 ggaagagaag atcttggtgc aactggggtt gctggtggag tggaacgaat aatgttctct
     9721 ttagaaaaac aagggattga tgttggcatc tctacaccgc aggtttgggt agcatttgtt
     9781 aatgatgaaa tgcaaaaagt cgcattgaat cttgtttctc aactaagaca gaaaggaata
     9841 gcagcagaag cagatctgat tggtaaacca ttgaaaaaac aaatggaaat ggcaagcaat
     9901 tcaaaatttg ctgttatagt ggcaccaaag gaatattcct caaactctgt agttgtacgt
     9961 aacatgaaag atggaacaga aaaacaaatt cttattgaat ctatattatc tgatccaaaa
    10021 tctaattttc aattctaaat gcgcgtgtaa gcataacaag ctccggtcca tttgttagag
    10081 tgccaaccac aagagatttg ttatttgcta atattcctga tgcaacgtac ggtataccac
    10141 cattaattgt agcggcttca agttccactc ctaaaacctc tgacataatc ttaatgtcgt
    10201 tttcttccgt atctgggtga actatgccgc cacgtgatgt agcaactgcc atggctcctg
    10261 cttgatgata tttagctatt ctttttcgta ttatttcaat tcctaatgca tcttctactg
    10321 cttttacaaa ttcacttgga atcataggtg acattatgcc tcctttgtca ttaacacaaa
    10381 tcaagtttcc aagtgctgtg tactttgtat ccaagactga tacattaagt cctgtagatt
    10441 ttttcagatg tgcaacttcg tcatctaaaa cagagttagg tagtattatg ccgtggttat
    10501 tgatgaccat gagaggccca agcagtctcg tgtttgcaac tgaggtgaaa atataatctg
    10561 tttttagaaa ctcagcaaga ttttttgctt ttgttgttgc aaaaccattt ggtacaaaga
    10621 caaaactatc gttaactttg gtatagatac ctacatttgg acttctataa acatcatatt
    10681 tgaaaattcc caataaatca aaacaatgat ctgaagatat gaatttatct tgaatacact
    10741 ttaattattc aattaggagt gatttacgat gccaatagat ccagactttc caaaaaatca
    10801 cacagttact ggaaaatcca aagatccttt gagtgataat catcatcttg tatggggtcc
    10861 tggcaaactt gctgaagctg cttccgatga aaatgtcaag gcagattatg ccacaaaggg
    10921 agaagaaatc aaaccgattg gaattcatgg tacatttgtt gcagtagatt gggactcgtg
    10981 cattgcggac ggaatatgtc ttatgagctg ccctgtcaaa gtttttgaat ggtacaaaaa
    11041 tcccggtaaa acactccgaa gagacagact gaattatacc gacaaggcta acccagtaaa
    11101 agaggctgac tgtatttggt gcatggcatg tgtagaggtt tgtccaacca aagcaatcaa
    11161 agttgatcag gctaatgtgg aaattcataa cacagagatc gctagactta gttcaaacta
    11221 attttgtcta ggtttaaata acatattata tctaaaattt tcatgccaat cgtagacact
    11281 tttccaaaag gatatgaagt aattggaaaa ataaaacacg cagatggcga acactttcac
    11341 tttgtatggg gtccaggtag aatggcagaa gcagcggaag atcctgcatg tatagaagca
    11401 tacaaggccc acggtgaaaa attagaacca attggtgtta gtggcacaat ggtcgcagta
    11461 gactgggatt cttgtgtcgg cgatggtgca tgtattgaag cttgtccagt acaagtcttt
    11521 caatggtaca gaacagaaaa agatattcct gcaataaagg caattaacga aacgtttgct
    11581 ggaactggta aaactgtaaa ggaagaaaaa ttagatcgtt ccgacaaggc agatccaatt
    11641 agagaacatg actgcatttg gtgcatggca tgtgtttctg tttgtccacc actagcaatc
    11701 aaagttgatc aatccaatct ggaataccat gaaaaagctg caggcacata tcagacatta
    11761 ggttctggta cagcaaatcc acacgcacat cactagaact tcaccttttc tttattttta
    11821 attcaattct ctagaggatt atttgttttt ttaaaatcta aaacaagaac tttttttggc
    11881 agatctattt caagcatttt ttctggaggt ggttcaaact gcaaaacgtg tatcaacata
    11941 gtagaatctt cttctatttt ctgattcatt tttttaatta ttcttttttg ctctatggct
    12001 atttttggct taaacaatac atcaaaaata tagtggtaaa aatttcccca aattctgaag
    12061 gaatactcta ccttgtagta tggtatgtgt aagacagggt atttcatttt agaagaagaa
    12121 tggagcagat cctgcagaaa atacactttg tggtgatagt catcaagaaa atgtgtttta
    12181 gcatatactg atgatctctg actctttcta gtcagaattg aggaaaactg tgaaaatgtc
    12241 tcatcaggaa gtcctgcggg gccatacttt cgatcatcat aaacttgtac tcttctattt
    12301 actacaaact ttgattccga ttctaaaagg cgtctgatat tctctatgaa ctgtggttca
    12361 ttgcactcaa aggacggcat ctagatctcc tagaattgtc ttaattgtac ttggtaaacc
    12421 acatattgat attataggat cctttggcca atagtcgcca agctcttcag atatagaaat
    12481 tgtttcttcc ccttttgcat agagcgtctt tttccagaat ttgccatcac tagttgttgt
    12541 tgcagtttct tgctctacaa atccaatttt ttgtaagcta ctacaaacta cttcctctgg
    12601 caaaaaaact gaaatcgact tgagtttttt ttcagacaac ctaatcaaaa tttacatttt
    12661 tgaaatataa acttggttat ctgaaaaaca ataaatagat gacaaatatg ccagcttctt
    12721 gaatgaaaaa gttagttatt gctatactat ttttgttgat ttctagttct actctgcctg
    12781 tatttgcaca acatcatgaa aatccgcctg cttcacttgg gaacaggatc gcagtgatgg
    12841 attttgtatt tccagaaaat attgcaatca atgaagattt tacactgaca atgatattgt
    12901 atgatgaaac taataagaaa aactttgaac aagtatcaat acaacttgct atctataaag
    12961 aagggatggg aaaatccctc atgaatgaat tattctatga taaaaatgga gaaattacaa
    13021 ttgattttaa acacaaagat tttgatcaac ctaataaacg agaaatcaaa gccgcggtag
    13081 aaccaattct tggaggctgg atgaaagatt atggcagcaa tattcagata ttgcaaaaca
    13141 tattttctga aaatgcactt tacacactaa aagctaccat tgtaacaata gaccatacta
    13201 gaacattcat cagcgaacca ataacattca caactgaaat ccacctaggc ggacatgggg
    13261 aggtatctca tacagaaatc gatatacctg actgggttag aaacaacgcc aagtggtggt
    13321 caaacggtga aatcgatgac aagacatttg caaatggaat ccagtatatg atcaaagaag
    13381 ggataataaa aatacccaca accgaaaatg aacaagcaaa taatgaagtt aaaattcctg
    13441 actgggttag aaacaacgcc aagtggtggt caaacggtga aatcgatgac aagacatttg
    13501 caaatggaat tcaatatcta gtcaaaattg gcgtaatccg tacttgaatc cctgattttc
    13561 tctaatccac ttctgtgata catgccgaaa atcttaaaca aattttaact agtttttatg
    13621 aacgaaatcc gcagaggtcg cctagcccgg catggcgtgg gccttgagag cctatgtgcg
    13681 caagcacgcg ggggttcaaa tccctctctc tgcgctattt ttctatcttg ccaatctcag
    13741 ctaaaagtgc tgaaagctga atctctggat ttgcaccaat aattagtcgg taatcatatt
    13801 ttgctatgac ttctaaaatc tgatcaagat tacttgtctt catcttgtat gtttcctcgt
    13861 ttaaatattt caaaaagtct gattcagaca taccataaac tttgatgagc tctatcattt
    13921 tatttctagc ctcggagatc ttgcctgcga ttgcaagctt tagaattcca ctaacatcac
    13981 ttgtttttgt aagcccagca gatgatttca catttggttc gtttacaatt ccaaggcttg
    14041 ctgtagcttg taaaatattt atggcatgtc gcatgtctcc ttctgaataa ttgtaaatag
    14101 tctctagccc tttttcatca aactttattt tttctttctt gcaaatttcc tcaagatgtg
    14161 atgtgatatc atcttcagaa attcttgtga atttgaatac ggcacatcta ctctgaagtg
    14221 gctctattat ttttgatatg ttattggcaa ttaaaataaa tctacaatat tttgcagtgt
    14281 cctctataat tctgcgtaat gctgtttgtg catctgaagt catttcatca gcctcatcta
    14341 aaattattat cttaaatgga atctttgtat ccaatcctga aaaacgcgca aattttttta
    14401 ccctatctct taccatgttt atgccacgtt catcagacgc attaagctca agcgtatatt
    14461 ctttccaata atctccaagg atctcccttg ctagacacat ggccgtagtt gtttttccta
    14521 ctcctgcaga accagaaaac atcaggtgtg gtatttcacc tacatttttc aaaagtgatt
    14581 ttaggctgcc tattacttct ttctggttta gtacggagga aagcttaacc ggacgatact
    14641 tttccaccca catggtagat tcagtcatag gctgatacct cggtttctca ctaataaatt
    14701 acgtctaaga tcaaatgatc aaagctactt gttaacggaa ttgatcgatc gcttacatta
    14761 aatgcttgaa cagagcttga ggcatgttgg aactctcaga cctttcccaa gtccatacag
    14821 taggatatcg attacaggat gttaaagtca cttttaatca tgatatgaaa attgatgcag
    14881 ctgaagtatc cttagatgca aaacatggtg aaattctaag cataccgcgt tgggtagccg
    14941 atgtacttgc atcagaaaaa cttgtagatg ttcaggatac tgatatggtt gttgcgttaa
    15001 agcaagcact agtaaaggaa aatgtacaag gagattttga tctttctaca cttgaatctg
    15061 atttttacat caaagtacga tcttatatga aaagacttcc acagcaggat tctgacaaga
    15121 ttgaaagtat gttgaataca ttgatgcgaa aaagacaagg aaaaataata aaacttgcag
    15181 actcttctaa gttaacatct gatatagcaa aaaaactatc aatagaggaa cgtgcccttt
    15241 ttgattcagt acattcaaaa agcatagaat ttcaaaaaca agtcttgggt gataaaaaat
    15301 gactgcactt tcagaacctg tgtcaacacc ggaaaagcga tcagatcaag ttaaagaatt
    15361 tctgactcag tttaaggata aatctggtgt ttacaaatat gtagaagaaa tagaccagat
    15421 gatggcaaaa aaaactcaat atcttgtagt tgattacaat gatcttgtat cacatccaga
    15481 aatagaatct gtttttaaca ctgatcctga tgaaatgtta tctgcctttg cgggcgcaat
    15541 aaaaagcatc cttaaggaaa gatttccgca atatgccgag aaaatacaat atgatgtaag
    15601 agtaagaata gcaaattatc cagcacaacg cagcttacgt gagataaacg ccgaaatcat
    15661 tggaaaaatg acaagtgttt ctggaatggt tgtaagatca tctgagataa agccgttagc
    15721 aaaagaactt gtttatctat gtcctgaagg tcaccaaact acagtagttc atgaaaaagg
    15781 actagagttc aatgaaccat taaaatgcag tgaaggaaga tgtactcata aagatctaga
    15841 gctcagtcca gaaaagagca gatttattga ttttcaaatg gtaagactac aagaattacc
    15901 agaagattta ccaccaggtc agcttccgca ttatgtagat gttacagtgc ttcaagatct
    15961 ggttgataat gcaagaccag gagatcgtat aatactcaca ggtattgtaa gaatcgaaca
    16021 ggaacatatt ccatcaatgc gaggaaaaag tggaatttac agattaagga tacagggaaa
    16081 taacattgaa tttcttggag gtagaggaac caaaacatca agaagtactg aaagagaaga
    16141 aatttctcca gatgaagaaa agataatcaa atcgcttgga aagaatactg atatctatga
    16201 cagattaatt gcatcatttg cgccacacat tagcggacaa gatctgataa aagaagcaat
    16261 tctgcttttg attgtaggtt ccacacaaag aatacttgca gatggagcaa aaattcgtgg
    16321 tgatatcaac atatttcttg ttggagatcc tggtactgcg aaaagtgaaa tgctaaagtt
    16381 ttgtgctaga attgcaccta gaggattata cacttcgggt agaggttcta ctgctgcagg
    16441 acttaccgcc gcagttgtac gagacaaaat tggtattatg atgttagaag ctggtgcagt
    16501 tgttctaggt gatcaaggtc ttgtttgtat tgatgaattt gacaaaatga aaccagaaga
    16561 tagaagtgcc ttacatgaag taatggaaca acaatctgct agcattgcaa aaggtggaat
    16621 tgtagctact ctaaatgcga gaacatcaat tttggcagca gcaaatccca tgtatgggaa
    16681 atatgatccg ttcaaaaaca ttacagaaaa tgtaaatctt ccaattccac ttcttacaag
    16741 atttgatttg atttttgtag tgagggatgt tccgtcaaaa gaaaaagata ccagaattgc
    16801 aaaacacatc cttaatctac atagaaactc agtacctgat acaaaatcgc tcatagatgt
    16861 agatattctc acaaaatact tgtcatatgc taaaaaaata gaaccagttc tgacaaaaga
    16921 agctgaagat aaaattctag aatattacat gaaaatgaga aatgtggaat cagaaggaat
    16981 gataactgtg actccaagac aacttgaagg attggttagg ctctctactg ccagggcaag
    17041 actcttgatg aaaaatcaag ttgacgccga agatgcagaa cgtgctattt tcttgataga
    17101 aagcatgctg caagacgctg gagtagatgt taatacaggt caagtcgatc ttggagtgct
    17161 tcagggtaaa ccacacagcg aagtctcaaa aatgcaatta ttcatggacg taatgaaatc
    17221 tcttgaaggc gacgaaaaac gaccagttga agaaaaagta tttgtcaaag agcttgcaaa
    17281 gacaggcaaa tttacagaag atgaggcacg taaatatatc aaaaaactgc aacgtgagtc
    17341 agctatttat gaatcaaaac ccggtcatta taaccgggta tgaaagtatc acaaattaat
    17401 ctaccaaaac ctgcaattga atttctaaac tctcttggat atgttgaact ctatccacca
    17461 caagaaaata gtgtaaacgc aggtttgctg gaaggaaaaa gcatactcgt gtctgcacca
    17521 actgcaagtg gaaagacgct tatcgcaatg cttgctatta tgaatcatct atttgcaaaa
    17581 aaaggaaaaa taatctatct cagcccgctt agagcattag catcagaaaa gttttccgaa
    17641 ttcaaaaaat tagaatcgtt agattttgaa agaaaaataa aagtgcaaat atctacaggt
    17701 gacttcgata tagcagacaa aaatattgag aaaagtgatg taattgtatt aacaaatgaa
    17761 aaaatggatt cacttattcg tcatggtgca gagtggattg atgatctaag ccttgtcata
    17821 gctgatgaag ttcaccttct tggagatcag gatagaggtc ctacccttga gatcgtgctg
    17881 acaaaactaa agttattgga acaaaaacca cagatccttg ctcttagtgc aactgtcact
    17941 aatgcggacg agattgcatc ttggattgga tgcgaattgg tagaaaacaa ttggaggcca
    18001 gttcctcttt cagaaggagt ttacgatgga ggcagagtga caatgcagga tggtaaatac
    18061 tttgaaattg aaacaagcat gcgtggacca tctgtagatc taggcataga ttctctaaaa
    18121 aatggaggac aaacaatttt gtttgctgaa acaagatctc gttctgcttc acttgcagca
    18181 aaggccgcag acgttgtagg taaaagtcta ccggaagatg taaaacatga tcttgaggca
    18241 atatcacaag aaattcttga tgacaacgag cacactgatc taataaaaaa tctcgctact
    18301 cttgtgaaaa aaggtgtggc atttcatcat gctggcctca atcaaaaatg tagagagatc
    18361 atagaatctg aatttagaag tggaaaaata aaattacttg catctacccc aacactggca
    18421 gctggagtaa atcttccagc aagacgcgta gtaatatcta acataaatag atatgatgca
    18481 aaatttggtg gaaataaacc aattagtatt ttagagtata aacaactctg tggaagagca
    18541 ggtaggccac aatatgatgc atttggtgag gcaattattg ttggaaattc aaacagtact
    18601 gaattaattg attattatgt gaatggtact cctgaaccaa ttgtatcaaa actaaccgat
    18661 gacagagcac taagatctca tattctaagt ttaatatcta caagtcctgg aatcaaaaaa
    18721 gaggaaataa tagatttttt ttctgaaact ctagcaggct cgcaggttag aaaacccact
    18781 ataaaattta aggtacaaat caccttagac tatcttgaac tagaagaact gataaaagaa
    18841 aaaggaaata ggtttgtagc aacagaattt ggcaaaaaaa catcaatgct ttacattgat
    18901 ccgctcacag ctgtttcttt tagaaaatct ttacaaaaaa cttcaaaacg aaaaaatcac
    18961 acttttgggt ttctgcacct tattacaaca tgtgaggatt ttttccccaa attctcatta
    19021 cgaaataaag actacgaaac cataggaaca ctgcttgaaa accatgtatc tgaactaatt
    19081 gagagtattt cagaatacga ttgttcacgt agtcttcttg caatgcatgc ctggataaat
    19141 gagtccagcg agatatttct atcagataat ctgggaatag aatcaggtga catgcatagg
    19201 atggtagaaa atggtgactg gcttgtatac tcactatatg aacttgccaa gctagagaaa
    19261 agagatgagc tcttagatga attgaacact ctcagacaaa gaatctctta cggaataaaa
    19321 gaggaattga tagagctagt aaagatacgc ggtataggca gaattcgttc acgcgcactt
    19381 tacaaaaatg gcataaaaag tttggaagac ctgtctaaaa ttccaataaa agaactagca
    19441 aaaatagaca aaataggcgt atctgttgca gaaaacataa agacacaatt acaaaaggta
    19501 tcatgatgct tccccagatt gttgcagccg tgtcagtttc aatcatatct ttctttgtgg
    19561 tttacaccat tactccattt ctaatacgtg cattagaaaa aagaaatctt acagtaaaag
    19621 attacaatag agtgggagga gctatggtgg caagacctgg aggtccttct ctgataattg
    19681 gaattgtaat atctgaactt gtgttatatg cattttttcc aacaaaagaa atcatagcta
    19741 ttctcatcac cacatcactt gcattcttga ttggaattat agatgataga aaagttctag
    19801 gtggctggtt taaaccagta gcgcttgcag gagctgcaat tccaattatt ttgcttggtt
    19861 catatgattc aaatcttgca tttccacttt ttggttcatt tagtattccg gctctctatt
    19921 tagcaatagt gatattcata attccaatta ctggaaatac aattaattca attgatgttt
    19981 taaatggtgt agcaagtgga tttgtaacta ttgcgagttt ttctcttacg atatctcttc
    20041 taatacttca aaattatgag attgcaattg caagtcttcc actaggattt gtttcattgg
    20101 cattttataa atatcataaa tttcctagta gaatatttcc aggcgattct ggcgccctta
    20161 ctcttggtgc aatgtatgga gtgattgcaa tagtaggcca tgtggaaata attgcggctg
    20221 tagcactttt acccgcggtg ataaattcat ttttattttt atctagtgta aagcgcatag
    20281 tagaacatag gcagataaaa tcaaaccctg taacccatac tgatgacttt aaactaatgg
    20341 ctactacaga caaatctgca ccagttacat tggttagatt aattcttaca tctggaccta
    20401 tgactgaaaa acaagttgga tttgtaattt tcaaactagc attattttct gcaattcttg
    20461 ctataattac aacgtttatg atggaaataa aaatatgaaa gaaatttttt tatttattat
    20521 tgtaatgtgg ttactaatca taggtggtgg ccaattgttg attacggtta cctcacaaat
    20581 ctcaatagat ggatatctga catttggatc ttcacaattg tattatgctt ctatggtaaa
    20641 agctgttgtt gcaatagttc tagtaattgg ctggatttac gttctcataa agatcaagaa
    20701 ttggatgttc aataaacaga tcaaaaatta actattttcc catgctttct tttttttatc
    20761 atattcctct ctggaatatt tgactttggc aggtactcct gctactaccg tatttggagg
    20821 aacatctttt gttactacag ctcccattgc aactacgctg tttttaccaa ctgtgactcc
    20881 tgctttgatg actgcccttg aacctattac tgcgccatct tctactgtaa ctccaatcat
    20941 cttcttgctt gtagggtatg gatcattggt aagtgcagcg gcaggaccta tgaagacatt
    21001 ttttcctatg cgggaaagtg gaggtatgta gaccattccc tcaatcaaag tattatcgcc
    21061 tatctttaca ttgtagtcta tgtgtgcaag tgatcctatc tttacattat ctccaatctc
    21121 tgtattgtca ccaacataag aaaaatgcca tatcttgaca tttttgccta ttttcgcttt
    21181 ttctgaaata aaatttgtta ccatttatct attcacaaat gccatgggga tgcttgtttt
    21241 gttatcttct ctggtagctt tgattgattt ttcttcaaaa attcaatatc ttgatttttg
    21301 tctcttaatt catctggtag cattattgga atttcttcta ttatgggata aaatctagaa
    21361 cacttcgtac aataaattgc accttctgat acattttgat cctttgaatc agattcaaaa
    21421 atctcgagag gaaaatgttt gtccattgga catgccaaaa tctccatcat ctttttgttc
    21481 atttcaaatc tagataaatt ggagttccct tttgactaga taaaagtgct gcttctgcaa
    21541 tttttgttgt gtttactgca tgctctggtt tcactaatag ttctcctttg ccttctacag
    21601 acgcaaggaa gttctttatt tctaatagca gtggttcttc aacttccttt cttggtattt
    21661 ctgcaccatt atttgtttca actttcactt cctgtgaaat gaaatctgaa gttattattg
    21721 catcagtaca gactgcatta aattttctca ctcttgttgg cgttatccaa tttgaagaaa
    21781 tgatagctat tctactatct ctgaaaccaa gcattattgt tgcaaaatcc tcatgatcat
    21841 gtcttatctt tccagatctt gcaaatacta cttcaggggt atcgtcaaaa agccacatgg
    21901 ctgtatcaat atcgtgcacc gaagtatcat aaattatgcc tacatctttt atgtgcaaag
    21961 gcattctatt ttctctgtga aattcaagca ttattaattc tccatatttt tttgatttga
    22021 catattcttt tactagtgcg actgccgggt tgaatctttc aatgtacccg caagtaagaa
    22081 ttaccttgtt cttttgtgct aattttaata attcttctcc ttcatgagaa agatacgtca
    22141 taggtttttc tacaaacaca ttcttttttt gttgaagaag ttttgtagct atggaaaaat
    22201 gagttgaagt tggagtgcaa atgaaggcgg catcaaattt ttcggaatct aagagtgatt
    22261 caattgatga atagtgattt acagaaaatt ttgtcccaaa ttcttttgct ctaccaagat
    22321 ctgcatcaca tactgcgctc agtaccccta gttgtgaaag aactctcgcg tgatttttcc
    22381 cccatcctcc agtgcctatc tgaacaactc tcatgtctag tatacaccag ttatccagtc
    22441 tgaatacttc tcatttcttc cagatgttat gtctgaatat agcgcgcgca tttgttttgt
    22501 tatctttcca accttacctg ttcctatttt tttgccatct acagtaatta ctggggttat
    22561 ttctgcggca gttcctgtaa gaaaaatctc atcagcaaga taaagttctg tacgtagaat
    22621 ctctctttct atgacttcat atccaaagtc atctgctaat gtcataactg attctctagt
    22681 aataccttca agcgcagatg agcttataga cggtgttatc actttgccat ttcttacaac
    22741 aaatatgttt tctccaggcg cttcactaac atttcctaaa tgatcaagca ttattgcttc
    22801 atcataacca tgtcttttgg cttcctgtgt tgccaaaata gaatttaggt aatttccacc
    22861 catcttggcc agtggtggtg ttgagttgtc actgatgcgt ttccatgacg aaatacctgc
    22921 attaattcca tttctgttaa atagatcacc aaatggaaac atgactatag ctacatgagt
    22981 aggagttttt tcatttacat tcaagtttat tccatattct ccaacaaaat aaaacggtct
    23041 gatatagcat gactgtctaa ctgagttttt tttgcagagc tgaattattg catttgaaat
    23101 ttcatcatcc gtaaatttta atgaaatgga atacactttg ccagagtttc tgaatctttt
    23161 aatgtgatca tttaatctaa agatattaag attttttgaa ttccaataac ctcgaaggcc
    23221 ttcaaaaacc gaagttccat aatgtatcgc atgagtcata acggaaactt ttgcttgttc
    23281 ggattttaca aatttgccat cgaaccaagc aaattctgca gtaaaagatt tcatataaaa
    23341 caacgaatta gtctgtaatt aatctatttc tgccagaacc catgttttgg gaatttcatg
    23401 ccaagaattc tatatgtaag atcttgtgaa tttgaatact tctcgaccac atcccaattt
    23461 tcatgtatta ttttaactac ctctgaagga acatagtttt cccaacctgt gtcttttcca
    23521 agtattgctt caaacatctt ttcctttact aacgatgcag agatcgtttt ccttgttcct
    23581 acctttgggt taagtccata gagaccaagc acaaatcctt ctgccttatc tccagtatat
    23641 gtaaaatatt tgtctgaaat accactcaag atttgttttt ttatcttcca agaggctggt
    23701 gataaaaatg gaggtagata cctaataaat ggtgcataaa atgtataatt tgaaagaaca
    23761 gaaacagaat caccaaatat tgcatggatc atctttcttc gtaattcaaa actaaaagga
    23821 aagcttcttg tgttgatctc tttttccttg ataaatacaa ccggcatgac aaccacttgg
    23881 ttttcttttt tcaactcctc tataatttcc acatgtgctt tggtaacagg attcaagtga
    23941 gcaagataaa ttgcaactgt caatagcatt catagtagaa atctagtcat atttcaatgc
    24001 ttgatctaat tctcacatat cttgataaat aatgagaaat aatcacattc tctacattta
    24061 gattttttct gcaagacgat ttatgcacaa gactaataaa ttgtaatctc atcactcaat
    24121 tcagaaaatg aatttaaaaa ttctttcatt gctagtaata ttgcccttat tgttaatgcc
    24181 agtggtatca ggttacgcta tgccatctga caaaacaact atgaaaaaac aaactgcgac
    24241 aatgtcaaac atggaaaaaa tgaagcacac agaacacacc agtattcaaa attcctattc
    24301 tttcctgatt gatgaaacaa cgataaaaac aaaacgtgga actgtaacac tttctggtca
    24361 cggcgcatat agtccagatg aagatatgat ctcggctttg ggaaaatact caattaaaat
    24421 gggtagcagg attgttactg gaaactggaa agcagttgga ctggttgatg gtaaaggaaa
    24481 tccgtatggc ggaagctctg atgattcaca tgtacacttt attggtaaaa ctgcaggtct
    24541 aaaagatcca aagtatacag gtaataatgc aaaacgagaa tcaataacgc gaggttcaca
    24601 ccaaatttgg atttctgcaa atgcagcaga aggaacacta tgtgtttatg gtggctatgt
    24661 gggtctatct actccaagaa ctgcatgtgt tgaaacagac acaatttcaa taacaaaata
    24721 attcttcaaa aaggtgggga cgatgggact tgaacccacg acatccagcg cccgaggctg
    24781 gcagtctacc aagctgacct acgtccccgt agatcagcct ctgactatac aatattagct
    24841 tttcaaaaag aaaattttct tctaaccgta agactcgtat tttatttcaa agctcttatc
    24901 tggtctcttg tttctttcac taacaaaacc ctttggagta agataatcca tacacccatc
    24961 aatagttccc tgccatccac aaatgaaaaa gactgtattt tctgctgtaa tcttgtctcc
    25021 tacaagtttc tcaagcggag atggttcgcc gtcttttgtt cgaagaaaag tttctactct
    25081 accagtttga ccgctccaag atctattgaa ccattcttga ggcctactga tggctgctct
    25141 atatctgaaa ttccacttgt ccttacctct atcaagactt tcattttcta aatcagtcaa
    25201 aagttctcta tatccaagtt cgtccacata acttgctccg tgtagtacta taagctccct
    25261 tttacttccc gtggcatgaa gagactgtgc aagactaaca aatggtgcaa gacctgtacc
    25321 tccaccaaga caaactattc gtctatcatc tttttcacca tttggttttt tatcatcaag
    25381 tgtaaaaaga ccagttggtt ttacccaaac aatctcgtct ccttcttttg cactaaaaag
    25441 ctgggtagtt aaacgacctg gaagtggctt tctaacccat cttatgtaca gctcaacata
    25501 ttttctgttt tctggatttg atgcaatgga ataagctctt cgtatgatct tattctcgct
    25561 tggaacgtgc attcctattg ttatgaattg accagatttg tattcgggaa caggaccatc
    25621 ttttgggacg attctaaaaa ttgcaagatc ttctttttgc acttgaacat aagtcaatgt
    25681 agctttgttg tcaacaacca cgcgaaaagt gctctctaag atagttaaat atcttatgtt
    25741 gatttgttaa ctctgatttt ctatatacag ctaaacgtta ataaccgtac gaaaaaagtt
    25801 attgcaaatc agactctgcc actctgatta taaaaatttg ggccggtcgt ctagcctagt
    25861 aggatacgcc cttggcatgg gtgagatcgt gggttcaaat cccacccggt ccatcttttt
    25921 ttaattacat tccctaaatc attttcttaa aactgataca tcagatcaga atctgtttta
    25981 cattggtttt caaattggaa ttattaagtc acagttacgg taaatgttga tttagtgtct
    26041 aatggatcta tatcgcgtaa gttattccac acaaatgttt caattttata cgttccgtag
    26101 ttgtggggaa tccaggaaag agatgttgtt aaagtttgat ttgctaaaag tgaacctgat
    26161 gaaaaactaa gatctacaac ggcatcatct ttatcgatta tttgtactat gtatacccaa
    26221 tccatttttt gatcactagt gtttctaagt gaagtttgta agttaagttg ctgttctaca
    26281 actggtgagt taatttcttc tcccaacgta ttgactattt taaattctga tgcagagatt
    26341 tctccatatc cataagcata tccaaaacta atcccaagaa aaattaaaaa agataaaacc
    26401 agaaaattca tcgaaccaat tctttcattc ttagatttaa attatcctac actgtagcat
    26461 ctctcatgtc cgagtctaga gagactatct tcataggcaa aaaaccactg atggcgtatg
    26521 ttacatctac gctaatacag ctggcaaacc aaccaaaaat atctatcaag gcaagagggc
    26581 taagcatagg tcgtgctgtt gacgttgcac aaatagttgc acgaaaaaca gagaatgctg
    26641 gctattctat cggaaacatc aagatcggat cagaacaact tcaatcacaa gatggacgat
    26701 cgaggaacgt ttccacgata gaaatcgaag taaaaagaaa cacttcgtag gaaacccttg
    26761 aaggattctt ccttcttttt gttattttgt tctacggctg tatttgtttt ccatttttct
    26821 aaattttgat aaatccacta tgtcgttgaa ttgctcgaat tcgacaagat tttttttgaa
    26881 tttcactgtt gttccagttt ttttcaattc gcgaagaatc tgtaaagatg caaatgcgtt
    26941 tgcaaaaaga acagaaagtg gatacaaaat tagcttaaat ccaattttat gtagtacctt
    27001 tgctggtaga atgggggttg caccgccttc aatcatgttt gcaacaagcg gcgcatctat
    27061 tgatcttcca atttttttca tttctgcaat agatcgtgga gcttcgatga agactgcatc
    27121 tgcacctatc ttttcgtaac gtagtccacg ttctatagct gcatcaagtc cctctgtagc
    27181 tcttgcatca gttcttgcaa ctattatgaa atctttgctt tttcttgcat ccaaagctgc
    27241 atgcaatttt tctgcatatt cttcttttgg aattacttct ttgcctgaca tatgcccaca
    27301 tctttttggc catttttgat cttcaagaaa tatcccagaa gcccctgctg attctaactc
    27361 tctcacaatc ttccaaacac tgagtggatt accatatcca gtgtctgcat ccacaatgac
    27421 gggaaccgat actgcctggc aaattctacg tgcgttttct attgtttcag tagatccaat
    27481 aaatccataa tctggcattc ccagtaacgt ggcagaagta ccatatcctg tttgaaacat
    27541 ggcttcaaaa ccggcttttt gagcaatctt ggctccaatt gcatcataaa ctccaggaac
    27601 tacgagtggt tttgaactgt catacaataa ttttctaagc tttgacaact ggtgatagtg
    27661 ttatagacaa ttatttatgg tttaatctat cttgatctct ttaccacggg attttttttc
    27721 ggcagattct agtttttgca tctctgattc caaaaatttc ttgtattttg tagtttcttc
    27781 ttttgtcatg tttgctacat ttgagctaag tgaatttcct atactggtta ctcgttcaag
    27841 aagatccatt gcaacaaact ttcctatact tccaactcga aacatcacgt caagctgatt
    27901 ttttactatc ttgtttaatg tttcaagatc atcagtcatt gatctacctt ttcgtgttat
    27961 ctgatatttg ccgtctttag tctcttcgat aagattttca tctagtaatc tcccaagcaa
    28021 gggataaatc aatccgggag aaggcttcca tttgcctcca ctctgtaatg ttgctgcatc
    28081 tattatctcc ttaccggtgc ttggattttt tttcaacgct tctaatataa aatatcttga
    28141 aaatcctctt ggaactgaac tgccaaccct ctgtatccat tgtgatagca tcactagtga
    28201 tatcgctagt gatatatatt aatgattcgg atgaaagttc tgctacgaaa atgagttttt
    28261 gctgatcctt cataagacat atttatctaa tttgaaaagg aagaggtcta aacatgaccg
    28321 acacgataga acacgattta ttaattgtcg gctctggtct tgctggtcta agagctgcaa
    28381 ttcaggctgc aaaaacaagt tcagcgatca agatagcagt aatttcaaaa ctacaagtaa
    28441 tgcgttcaca ttctgtttca gcagaaggtg gaacagcagc tgtacttttt ccagaggaag
    28501 gagacagcct ggaatcacat atttatgata ctgtaaaagg aagtgatttt cttgcagacc
    28561 aagatgtagt agagcgatta gtccatgaaa tgccatctga aatttatcag ctagaacatt
    28621 ggggaatgcc ttggtctcgt cgagaagatg gaaaaattgg acaaagaaaa tttggtggtt
    28681 atagctttgc aagggcaaca tatgcacaag ataaagttgg attctatgaa atgcagactt
    28741 tgtatgatac atgtcaaaaa tttgataata ttgaatttta caatgaatgg tttaccacgt
    28801 caataattca tgatggacag aagttttgtg gtattactgc aattgagctt tcatctggaa
    28861 ccttttacac aataaaaggc aaagcactga tcattgcaac cggtggtgct ggtagagtct
    28921 acagctactc gacatacgca ctttcctcaa cccctgatgg tctagatatg gcatacagag
    28981 caggtatggc tctaaaagac atggagtttg tccaattcca tcctactggt attttgccat
    29041 ctggtatact gattacagaa ggtgcaagag gagaaggcgg atatctcctc aacaaagatg
    29101 gggagaggtt catgaaaaga tatgctcctg gattaatgga gttagcacca agagacatcg
    29161 tatctcgttc aatgatgact gagatgaagg agggacgtgg ttttaaaaat gaaacaggag
    29221 ttgattgctt aaaactagat cttagacatc ttggtgatga tattatcaaa gaaaaacttg
    29281 ccgcaattag agaaatttca atgaagtttt ctggaataga cccaagtaaa gaaccaattg
    29341 acgttagacc tgttgctcat tatatgatgg gtggaattca tactgacatt gatggtgcca
    29401 cagaaataca aggcatctgg gctgcaggcg aagctgcttg taacagcgtt catggtgcaa
    29461 acagacttgg agccaattca acaagtgaat gtctagtatg gggcaaagtt actggagaac
    29521 ttgcagcaaa atatgtttta gatgcaaaga tggtaccatt cccattacat cttgtagctg
    29581 ctgaagaaaa aagaatctat gatggaatat ttcgtggaag gggtgatgta aatccatatg
    29641 aagtaaagca agaattaact gatactatga acgaaaaagc atatgtcttt agaactgaaa
    29701 aagatatgat agatggacta aagaaactgc gtgagctaaa agaaagaaca tggaaacatg
    29761 tagatgacaa ggcatctgaa tacaatacga atttcacaaa tgtaatggag cttgattcta
    29821 tgtttcgagt tgctgaagtt attctagttg gagcaataaa gagaaaagaa tctagaggat
    29881 cccacgcaag aattgattat cctgcaagag atgacaagaa ttttttgcac catactctgg
    29941 catattatga caccaaggaa ccaatcctaa aggaacatcc tgtcactatt actaaataca
    30001 agccagtgga gagaaaatac taaatggaaa aacagtcaaa tcgtgaaggc attaaaggct
    30061 ggttaaatcc aggtagattc ggtttagaac gtgttgcata ttggctgatg cggcttactg
    30121 gacttggttt acttgtatat ctaattgctc acgtgtttga aacaagttct atagtaaatg
    30181 gtaaagaagc ttgggaaaaa atgcttgcgt taactcaaac tactgaaggc cacatcatac
    30241 ttgccatagt gattggaatg tgtgtctttc atactgcaaa tgggattagg gtgatgcttg
    30301 gtcatggtgg agtaggagta ggtaaaccag ggcgaccaga ttatccgtac aaagctgcat
    30361 ccttaaactc tagacacaaa ctatgcattt tggcttctat aggactagct gcattagcaa
    30421 tgatgtatgg attggcagta ttatttggtg aataaacatg caagaaagtg taataatgaa
    30481 aatacattac ggaacagctc ttggggcagt tgcacttgtt gcaattcaca ttctgtttag
    30541 aatgacgcag aacttttcag attctttaca gtatgaaagt gttattgcaa attatcagtt
    30601 cctgccatac gccataatgc tagaactagt actgatctta ctttccgttc atggcttcaa
    30661 tggattaaga gtaatacttt tggaattaaa acaaggtgta ggatatgaaa aagctgttaa
    30721 ctatggttgt cttggagcca tggctacact catagcttat ggctctagaa ctatattgat
    30781 gacaggattt ggtatgactt aagatggcag caataactcc aagaatttta gaagaagttt
    30841 cttcaacggc agtctctcct tctaatacaa taacgcttag aatatcaaaa cataatccta
    30901 aatctgattc atctacaaaa tttgtagagt ttaaggtgcc tgtacaaaaa tggacaactg
    30961 ttttggatac gatattggct gtaaagcaac acttggatcc ttcagtgtct gttagatatt
    31021 cttgcaggca agcttcatgc ggttcttgtg gaatgaaaat aaatggcaaa cctgcacttg
    31081 cgtgctatac aaaaatttct gagctaaact ctaacatcgt aacagtagag ccaatgaata
    31141 actttccaat aattagagat ttggttgtag actttactca gttattttca aatcataaaa
    31201 aaataaaacc atacataata aatgaagatt ctgagattaa atctgacacc aaagaattct
    31261 tacaaactcc aaaagatgta gaagaatatc tccaattttc atattgcata aaatgtggac
    31321 tgtgcaattc tgcctgtcct acaatggcta ccgattcatc atttattggt ccacaagcac
    31381 ttgctcaagc ttatcgttac gtcgcagaca atagggataa cggtaaaaag gatcgcctca
    31441 aaatagttga tgattcacac ggtatctgga gatgtcattt tgctggttct tgtagccaag
    31501 tttgtccaaa aggtgttgat ccagcaatgg gaatccaaat gttaagagga tacatgttag
    31561 gatttagaaa atagatttta aggtttttta ggattcttac ttgcatttac ttggttgttt
    31621 atggcttctg ccataaagtg atttgataca ttcaacttta catcgtcaac tccatcaagt
    31681 tttaataaat tatcatgaac atcctgtgct attttaaaac caaaaattgc tgggcaaaag
    31741 ggacttgtta gatgtaaatc tacctttact gctgagccac tgatatctat attgtctatc
    31801 aattcaagtt ccattatgga tacattgatc tcaggatcta caatttttgt caactcatca
    31861 aaaatctttc ttcttaattg ctgagtttcc tgtgccataa ataaaaaaac cgtctatgct
    31921 atatataacc attctaattt ctaaattgat gtatcggtct agattcgacg gaagtttaga
    31981 tgaacacaca ctaaattatc tttcatcaat ttcagatgat gttgaaattg caatttatga
    32041 tatagtgggc agtcaggctc atgtcattat gctccttgaa aataaaatta tcaccaaaca
    32101 tgaggctgta aaaattttgt ctgtgcttga aaaattaaaa aaagaaaatc tttccataaa
    32161 atccgattct gaagatattc atgagctaat agaatcacta gttatcaaaa aagcaggaaa
    32221 acaagcaggt ggaaagatgc atactgcacg atcacgaaat gatcaagttt cacttgatat
    32281 tcgcatgaaa atgcgagacg acattaacat aatatgtgcc tgtcttttgg acacaattga
    32341 aacgcttgta tcgttggctg aaaaaaatgg tgatgctgtt atgcctcttt acacgcatct
    32401 gcagcaagca cagattggag ttttttcgca ttatttactg gcatatgcag atgcattgtt
    32461 tagagatgta gacagattct atgttacata tggacgagta aatgaatctc ctctaggtgc
    32521 aggacctgtg ggtggtacaa gtttgcctat caataggcaa agtactgcaa agatgcttgg
    32581 attcaaaggc ttgattgaaa actcaatcga tgcaacaggt gcacgtgatt ttgttgctga
    32641 atatgtgggg catgttgcaa ttcttatgac aaacttgagc agattggcag aggactttat
    32701 tctttggtct acttcagaat tttcatttat tgaactttca gacaagtttg catctacgtc
    32761 aagtgttatg ccgcaaaaaa agaatccaga tatacttgaa cttacaaggg gaaaaactgc
    32821 tcaagtgatt ggataccaaa tggcaattct gtcaaacatg aagggactgc catcaggata
    32881 cagcagagac ctacaacaga tcaagacatc tatctgggct tcttcaaaga ttgcaatctc
    32941 tacacttcta gtgattaatg ccatgctaaa aaccatgaaa gttaacaggc aaaaaatgaa
    33001 aaaatcgata gattcaggat attctatttc actagatatt gcagagcatc tagttaagaa
    33061 aggagttccc tttagaactg ctcacaaaat agtcggcagc ctagtacaaa ctgcgtcaaa
    33121 ttcaaaaaaa gcactatctg gattatctct ttctgaaata agatcatcac taaaggaaag
    33181 tgacgtaaaa ccaaaagaac tatacgaaat catgcaatcc atgactgctc attcttccct
    33241 ctctactagg acgtctcaag ggtcagctgg aaaatcagaa cagctacgta tggtttctga
    33301 tagaaaaaga aaggttcatg cctatagggg aggcactgtc aaaaggacta acgaaatcaa
    33361 aactgccttt gaaaatctat cttccaagat aaaaataata ttaaaataac aggtaaaggt
    33421 ttgttattgt aaatttttta ttaaatttct agtactaacg gctaaatttg atgtagtaat
    33481 cttgactttt ccattgattt tgattcacat acctctagat aaaaaattcc cgtaatgagg
    33541 tatacagtat gtttcaattc atacaccaac aatccgaaaa acctaactac actgcaggtc
    33601 aaacctgggg agcactaaag aaagcgtgga gaggttacaa gattgccaaa gtgcagtctg
    33661 attcctcgaa aatgactgaa tatgctaaaa aaattagaac tctccaacac gatctgggag
    33721 taaagcaagc tgaattccca gaacttaacc tgagttagtt agccctcttt ttatttttgg
    33781 gtctaactag gtttatgatg ctacgcgcaa gtaatacccc aaatgtaaat gtggttaaaa
    33841 caaagattat ggttgatacc attagaacat tagtattatg aaaaactgtt ccaaatacga
    33901 tcaatcctat actggttatc attacaagaa tgacttttct atactccctg gtaatgtcaa
    33961 accttaaaga aagtggccac aagtatactg ctccgatcaa agtacctgaa acaaaaccag
    34021 ttgatatgat gcctatttct cccccgagca ttgttgttat ctctgatgtc tttagaattc
    34081 caatgagagg ataaagtgcg gtcttaatta ccacttgtaa ggctggattc tgcctttctg
    34141 catttgctat ggttggacta aaagaataat ataatgaatt aaaggcttgc aaaaaacttg
    34201 cacctgaaat tgttgacaaa aatttttcat cccgaatttc tcttaacatc tgtacttgtg
    34261 gcgcaagctc agatccaaat gcagctgtcg caatcaaaca tcctccgttt tttactccat
    34321 ttgtatcttc aattggatca tttgtagtta aaacaggttt gacaatttca aaaatatctg
    34381 aatcatatag atgcccccat ctggctgtta tgttgtattg tcctgcatca aacggtacat
    34441 tgtttggaaa agtaattggt aatctgttaa aatacggtgg ttcactatct acctgaactg
    34501 gtatatcacg ttttaaaaca agttcttgac cagttggatt tatcttgaaa atttctaatg
    34561 ttaaagttgt ctgatctgaa atttcagatc ctgaaaatat gatctctaat ccgatgtctt
    34621 ttagatcctt gattgtatat tcttgcctgg caacatctat atcaaattta tttaatgtag
    34681 ttatttcaaa tatggttgta gatcgtttac ctgcatacgt tgcatcaagt gcccatttgc
    34741 caaacaaatt accatactgt ctattgcttt cgacaaaata aatatctcct tcaagtttgg
    34801 caaaacaatt agttgcagtg atgtctgaac aaacagaata tgtatctgtg tatctatgcg
    34861 gtcctctaga atagattgac tctactacca ctttttcatt tggtatgatc tgtggtatct
    34921 ttgcaagtat tcgaatctcc tcatgtttgc tttgctcgta cgtataatga taaataggct
    34981 gatccgttga tatctcaaaa tcaaatgatt gtggtcctat ctgttcagag ttagtttgcg
    35041 ctgatgctat ctggatcaaa aaaggtgcaa tgatcataaa cataaaaaat ttattcatac
    35101 aagatctttt ttgcagctta taaaatgttt aacgggttcg cggggattcg gaccccggac
    35161 agccggatta aaagtccggt actctacctg gctgagctac gaacccacag cgaagtttgt
    35221 cggttgtgta taatttagtc ttttacaaaa ttttttgatt taatagaaac ctttaaactc
    35281 ggattttcgt ccaacttact gcaacgccct tgtagtatag aggggcatgg agaaattcca
    35341 ttcctcgtaa aacccggtct agaatggggc cctgtcacgg cctcgacgcg tgttcaaatc
    35401 ccgccaaggg cgccattaat ttttgataat atgtttgact tcgtagttga ttcatgcgga
    35461 aaaactgaat atcaattgcc tatttttaat ctctttacca tgtcatctaa aatttctttg
    35521 cttgcggcag caataaagga aagcttggtt tcataactaa gatcagaatc caagggtttg
    35581 ccattctgat caactacgta tcctcccgct tctaatgcaa tcagccgtcc agccgcaata
    35641 tctgttactc taatctttcc tcttaaatca ataaatgcat ccatcaaacc ttttgcaaac
    35701 ataccaaatt ccaatgcatt tgcaccaaaa tgcctgatgt gatttgatgt tgtaattatt
    35761 ggagataatc tctcaagtgt tactggtgag attccagata tatcgagccc aacaacaaaa
    35821 taaattggct ttttatcctt gtttacggaa attctatttc catttaagaa ggcacctttg
    35881 ccttttgttg cagaatacat atcacctctg aatagatcag ttaccactcc atctgtaaca
    35941 gtgctaagct tattttcagt agcaaaagca agtgaacagc aaaaaaaagg aattcccctt
    36001 acagcatttg ttgaaccgtc aactgcatcc atgatcacat atccttttgg attttctgaa
    36061 agtacaaccc tgccacattc ctcaccaagt actatgcagt caaattttat ttcttttaga
    36121 tagtcaagaa ctgttttttc tgcaatgatg tcgatgtttc ttgatatgtc tcctcctgca
    36181 cccctgccaa aatctcctcc cgattcctta gttcctgcaa gatgtcttac attattgtac
    36241 actcgcatag aagcctcttg cagtatttct attacttgca tatcgtactt cacttttgtc
    36301 ctaatttatt tcaaataatt tcaaaattaa ctcaatgcat aaataacaaa acaaaacttg
    36361 ccattatgtg agcggaaaag atgagtcggt ctttagtaaa gacgcacttt tgagcacaaa
    36421 acctggaaaa gaaattgtga aacaggctct tttcaagagt aaaggttaca aacaatttga
    36481 caaatacaaa aaacaagcag aggaagaatt ttcaaatttt gtaaacagat tcaccgatga
    36541 tctattaaaa gaaataaaat ctgatccctc cccagatgtg acacaacaga actttgcaga
    36601 ggaaataggc tccaatgaaa taattctgga aaagtcccaa acaaaccaga taaaatcaaa
    36661 actcgaaaat tatgatattc taaaagatag ggtctcaaga atcttgaatt caaattttgt
    36721 caagatgacc tttccagttt ttagcgcatt atatgatgca tcttcagaat attttggtga
    36781 taaacaagac attcagctgc gaaatgacat cattgatgga catattattg caatagactt
    36841 gagtgaacca atggatagaa tcatggacag agacgaagat ttggaatatc tggatgatta
    36901 caaactgatg aatccgtatc tgctaaaact tgcacgagat aaaatagcaa aaggtggcaa
    36961 agaagttctt gaggaatttg aacgaggatt caaagatgca cgaattggtc agtatctaga
    37021 tttcaaacta aagacaaaac caactaacat ctctgaagaa gacatgatgc agtgttataa
    37081 aaagtacagg gcagttatgg gtactgctgg ccgaaacatg gcactttcaa gaaaaccact
    37141 tgcagaaata ttctaccttg gtatggcaaa agcagcagaa tcagttggct gtggtaatga
    37201 aattgaggat tctataaaaa ataaatttgt taaaattcca tcatggcctc tttattatag
    37261 tctgcttact aatgatgtta aaaaaggatt tgaaattaca atgaaaaaaa gtaacttata
    37321 tctagatgag gctagacttg cactagaatt gctgcccgaa aacttttcac atagaaactt
    37381 tctagacttt ctatttctta cagtagaaca ttacaactta ttttggtaca atcaactgca
    37441 aaaagaaaat ctgtggtcag aattttctgc taaactaccc aagtgataaa catgatgtgg
    37501 tcagaaaaat atagaccaaa aaactttctt gatgttattg gaaatgaaga agctaaagta
    37561 tcaatcatag aatggctagg aaaatgggta aaaggaagta agccaatctt acttgttggt
    37621 cctcccggta taggaaagac cacgcttgca ttactttgtg caaagcaatt tggatatgat
    37681 ctaatatcgc ttaatgcaag tgatgtaaga agtaaacaaa aaatccaaga tgttctctta
    37741 cctgtactag gccacacaag cgttcttggt aaaccaatga tcttcataga cgaggttgat
    37801 ggtattcatg gaagagccga ttatggagga gttgaggcac ttgttgattt gctcaaagaa
    37861 cccacagtgc caatagtgct ggctgcaaac tatgaaagaa aagacaagat gaaaaacatt
    37921 aaaaaagtaa caaagatgat tcaactaaaa ccaattcctc cacgactttt acgactatat
    37981 ctggaatcaa ttctaaaaaa agaaggtgca aaactaggtg taggaacaat tctacgtttg
    38041 gtagtggaat cgcggggaga cattaggtca atcataaatt cagctcaagc ccttgtttca
    38101 ggatttgagc cacaaacaga aaagtcattt gtggaaatgg atgtagaaga agcagtaaat
    38161 gcatttttca aagctaaatc tgttgatgaa gcaagaatta tactatattc attaaggatt
    38221 gatccacaag agaaaattca ggcattttat tctagtgtga taacaagcag tctttcaaaa
    38281 actgacatgc aaaaaatgct tcaagttatt tcagaagcag acttactata tggaaaaata
    38341 agaaaaactc aagaatggag attgttgcga tatatggatt ctattcttct tggattatac
    38401 aaagaaggta cagctgcaag atattcacaa tataatctgc catggccact tttgaatagg
    38461 ctaagatggg acggaaaggc aataaaaggt atgatatcaa ttcttgcaaa aaagatgcat
    38521 gtttcaacaa gtgattttgc caccttttat tttccataca tgttattttg tatgaaaaac
    38581 aaatttcttg atttgaattt tgatgaatca tataatgaaa ttttacaaaa ggagatggct
    38641 gcactaaaat gagctggcgc aaaatgccaa tgaaattctc aggtatgtgt gtagtatgta
    38701 aaaagaaaat tgagataaat gaggtaggat tatgggctaa agggttagga gtaaaacatc
    38761 taacatgtgc tgaagtttta gaacttcggt gtgttatatg tggagggcct gcaggatgcc
    38821 ctcaatgtga attcagagat gattgtgatc tttccaaggt gtcacaacta tgtatatgca
    38881 aaaaatgtaa tgatccatct gattcatttg tatcatacca aaaagcgatc ggcaaaaaat
    38941 ttcctattct aaatcttaaa atttaatcca aaagagaaaa acactctgat gtctaaggac
    39001 ttcactgtac ctgattacaa gccaaaacat gttctgagtc cagaatttag gggtacatca
    39061 aactatgaaa taggactcga gtgtgcttct ttcagaagca aaagaaaatc aagaaaaaat
    39121 gagtgccgat ccagagacaa ctccaattac accgtgtggg agaggatctg aaaacattag
    39181 agtatttgta tgaaaactac tacttggcat gaatgtattt agaactgcaa aggatggacg
    39241 caaggtctca gagaataaaa ctacttaaat taatatagcc tgtgaatagt ttttaattga
    39301 aaataatgca cactgataaa attaaaaatt ttttacaaaa aagaagaact gcaaatcaag
    39361 gtggcgggca agacaaaatt ttatcacagc atgaaaaagg caaactaact gcaagagaac
    39421 gaattgatct tttacttgat gagggaagtt ttacagaaat tgatgctctc acaactcatc
    39481 actatcatga attcgaaatg caagataaaa aattctttgg tgatggtata attgctggtt
    39541 atggaacaat aaacggaagg caagtctttg tttttgcata tgactttaca attcttggtg
    39601 gaacactaag tcaaatgggt gccaaaaaaa taacaaaagt tatggatcat gctgtacgcg
    39661 ttggatgtcc aatcatagga atcattgatt caggtggtgc aagaattcaa gagggaattc
    39721 taagtcttga tggatttgct gatattttct atcacaacca acttgcatca ggtgttatac
    39781 cacaaataac tgcaagcatt gggccatcag ctggtggcgc tgtatattcg cctgcaatga
    39841 cggactttgt aattatggtt gacaaattag ctacaatgtt tgttactgga ccagaagttg
    39901 ttaaaactgt ccttggtgaa gaagtttcat ttgatgaact tggtggagca atgtcacatg
    39961 gaaaaaatag tggagttgct cactttgttg caaaaaatga atatcaatgc atggattatg
    40021 tcaaaacttt gttatcttac attccacaaa acaatacaga agaaccacct attgtttcaa
    40081 caggtgatga tccaaacaga ttagataata atctgataaa catagtgcca gaaaatccac
    40141 tacagcccta tgacatgaag gaaataatat tctcaatttt agataatcac acattttttg
    40201 aagtccatga actgtttgca ccaaacgtca ttattggatt tggtaggatg aatggcagga
    40261 ctataggaat tgtggcaaat caaccaatgg tgcttgcagg tgcacttgat attgattcgt
    40321 caaacaaggc atcccgattt atcaggtttt gtgattgtta taacatccca attgttactt
    40381 ttgtagacac gcctggctac atgcctggaa ccaaccaaga gcacaatgga atcattcgac
    40441 atggaagcaa gcttttgtat gcatattgtg aagcaacggt accgaaaatc acaataataa
    40501 taggaaaagc atatggtggt gcctacattg caatgggcag caaaaacttg agaacagaca
    40561 tgaattatgc ttggcctaca gcacaaattg ctgtacttgg atctgaagct gctgtaaaaa
    40621 ttatgaatag aaaagaaatt gcaaatgcaa aagatcccga agctatgaaa aaacaacttg
    40681 ttgacaattt cacagaaaaa tttgctaatc cttatgttgc agcatcccat ggcacaattg
    40741 atacggtaat agatcctgca gaaacaagac caatgataat taaagcactt gaagcacttg
    40801 ttaacaaacg agaaaaacaa cttcctagaa aacacggaaa cataaatcta taattaacat
    40861 gatcgaaaaa attctgatag cgaatagagg agaaatcgca atacgtgtga tcaaagcatg
    40921 caaaactttt ggtctaaaat ctgttgcagt atattcagat gaagatgtaa attctagaca
    40981 tgtcaagatg gcatcagaag catatcacat aggatctgct gccccatcac aaagctatct
    41041 taacatggaa aaaataattg aagttgcatt aaactctggt gctgatgcaa ttcatcctgg
    41101 atatggtttt ctttcagaaa atgctgactt tgctgatctt tgtgaaaaaa gaaaattgaa
    41161 ctttattgga ccctctggaa aatcaatgcg actttgtggt gataaaatgc tctgcaaaga
    41221 tgcaatggca aaagccaagg ttcctactgt gcctggaagt cctggaatag tagaagaggt
    41281 agagaaagca cttgacattg ctcatcagat aggttaccct gttttgctca agtctgtatt
    41341 tggaggtgga ggaagaggaa taagattagt ccataatgag aaagaactta gagaagcctt
    41401 tgaaattgca tcaggagaat cgaaagcggc atttggaaaa tctgcacttt ttgtggaaaa
    41461 atttctccca aaaatcagac acatagaata tcaattattc agagataaac atgggaacgc
    41521 tgtacatatt tttgaacgag agtgctctat tcagagaaga caccaaaaac tcattgaaat
    41581 gtctccatca cctgtggttg atcaaaagac acgggatgaa attggaaagc tcgccatcaa
    41641 cgcagcaatt gcagttgatt atacaaatgc tggtactgca gaatttctaa gactagatga
    41701 tggaacattt tatttcatag agatcaacgc aagacttcag gtagaacatc ctgtaacaga
    41761 acttgtttca ggcttagact tggtaaaact acagattgat attgcaaatg gaaaagaaat
    41821 tccattcaaa caaaaagact tgaagctaaa aggttgtgca attgaatgta gaataaatgc
    41881 agaagataca tttcttgatt ttgcgccatc tacaggtcca attcctgacg tgaacattcc
    41941 gtctggtcca ggagttcgcg ttgacactta cctttaccca ggttgcactg tttctccata
    42001 ctatgactcc ttaatggcaa agcttgtcac atggggagga acctttgaag aatcacgaca
    42061 aagaatgcag cttgcattat cagattttta tattgagggt gtagaaacat cgataccact
    42121 ttacaaaaca atacttgata g
//
LOCUS       DATIDL010000138         5360 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrosopumilaceae archaeon isolate SMAG_U16342
            k141_574498, whole genome shotgun sequence.
ACCESSION   DATIDL010000138 DATIDL010000000
VERSION     DATIDL010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935836
            Sequence Read Archive: SRR25158326
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosopumilaceae archaeon (soil metagenome)
  ORGANISM  Nitrosopumilaceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrosopumilales;
            Nitrosopumilaceae.
REFERENCE   1  (bases 1 to 5360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/04/2024 05:09:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,520
            CDSs (total)                 :: 1,474
            Genes (coding)               :: 1,474
            CDSs (with protein)          :: 1,474
            Genes (RNA)                  :: 46
            rRNAs                        :: 1 (5S)
            complete rRNAs               :: 1 (5S)
            tRNAs                        :: 43
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5360
                     /organism="Nitrosopumilaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_574498"
                     /isolate="SMAG_U16342"
                     /isolation_source="soil"
                     /db_xref="taxon:2202732"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..661)
                     /locus_tag="VLB45_06145"
     CDS             complement(<1..661)
                     /locus_tag="VLB45_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016939398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HSD05313.1"
                     /translation="MRIAIASPEHNDQAVKTVRTILEGHGIDSYLLGNKTVIKDTDCV
                     VVTGGDKGVRHYFHNILDSETPVLGINESESSGFLAQIDLKEFSSFVNRLKNKDFTVE
                     KVSRIGLKINGKSIYPALNDIAIFPSKSAMLMEHVLRINDEEVWHDSSDGVIISTPIG
                     SSAYSMSAGGPMIFQDSKVFGIVSVNSLDITRRPLIVSEDSFIEFDNISSRLHCEVIL
                     DG"
     gene            complement(696..1571)
                     /locus_tag="VLB45_06150"
     CDS             complement(696..1571)
                     /locus_tag="VLB45_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HSD05314.1"
                     /translation="MKIDIFSHCTIDEIVREGSVIETAGGPACYCGLTARNLKFDVEF
                     HTKIGPDFTHREFLEKNKIIIPEISFSQNPTTRFLLEISGIDRNLYLKTKCDPIEFDG
                     LHTDGCIVSPVFDEISYDTLEKIKKDSKFTLLDPQGFLRRKDQNNKIYLEKTDMNLSN
                     ISAIKVDVDEAYALTGQSDKDAMRALQKKGIEHVLLTNKREITVLVKDRLYYLTIPNT
                     EVFDTTGIGDIFCASFACTYLKEKDFFWAMCFAVGAAQAALETKKTGLEKIPQRGAIE
                     TNASYLYNLVKFSQI"
     gene            1782..2069
                     /locus_tag="VLB45_06155"
     CDS             1782..2069
                     /locus_tag="VLB45_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S26e"
                     /protein_id="HSD05315.1"
                     /translation="MPLKRASRGRQKGGKGSAGRIQCTNCGATVPRDKAKKVTSRLNL
                     VEHTLAKELRAQGAYIAAPTVLKWYCISCAIHFGILKIRSADSRRNHGRLR"
     gene            complement(2070..2642)
                     /locus_tag="VLB45_06160"
     CDS             complement(2070..2642)
                     /locus_tag="VLB45_06160"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HSD05316.1"
                     /translation="MLNNFRESLKPTLQKIGIVFAATGLSANFWTAVGLAFAFASAVA
                     YAWHFEYAFVVGGVMLLVSGFFDIVDGQVARITKKTSKKGAFLDSVFDKIAEVAIFLG
                     ILIGGYAEGYVVLLAITLSLLVSYTRARAESLGVQLQGIGIGERAERLLVIAIIGMAG
                     FLNYAVIIVVIIAGITLVQRIIATARAIKE"
     gene            complement(2696..3250)
                     /locus_tag="VLB45_06165"
     CDS             complement(2696..3250)
                     /locus_tag="VLB45_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HSD05317.1"
                     /translation="MSTTSLRKDHDLIEKVLKSMWAALSLLKEGKAIPESILGPVIDF
                     TKNFTDVCHHGKEENSLFPELEKKGIPRNAGPIAVMLMEHEITRKISHRMEESSKDYL
                     QSGNATQLIVDMTEYINHVMQHLWKENNRLFMMADMALQDKVDQVNQSLTMVEESKLK
                     EIGKSRENYENMAEDLDKKISTIS"
     gene            3306..3518
                     /locus_tag="VLB45_06170"
     CDS             3306..3518
                     /locus_tag="VLB45_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010192750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05318.1"
                     /translation="MGIIAKGAKCNVSGCGNDGIRSLTTSKVEGTGLSISSSSKKTVL
                     CKEHYKEWKKATKQDRDVERARYDRF"
     gene            complement(3575..4225)
                     /locus_tag="VLB45_06175"
     CDS             complement(3575..4225)
                     /locus_tag="VLB45_06175"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF012630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031012 - extracellular matrix [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HSD05319.1"
                     /translation="MHVKLLSSLIMIFLLGNIMSFNDSFAMKEQISDLQKKISFFWME
                     GKISDKEYIDAIQYINKHILQKKTPSEISANDLSKNKIASVRFMYVEPIPEWATNTKD
                     SLSDAIQFWKKTTNTEFKTTTSKNDSTIVIKWIREANPYHAGYLIDNKIIEIGVGYSC
                     NGKWHQYDSDTLSMLLKHELGHALGFKHSSDPKSIMYPTISAVSYVNHYSQYANAC"
     gene            4475..>5360
                     /locus_tag="VLB45_06180"
     CDS             4475..>5360
                     /locus_tag="VLB45_06180"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HSD05320.1"
                     /translation="MNKLLFAWFVSILCIIALGSLENDAIQTLVYATHTDENERQYET
                     KPSDILDEILSWYFLNLKINTLTIFGDIKMQDGFIGNTLDLDGINDYLVVHSTSDTNQ
                     FKSMTLSAWVKPNYTRASHQLTVISKDKAFEVSILNQKSNSFALFSVYDGMKWVTVKS
                     TSPISEEWTNIIATYYGSTLSIYVNGNLEATKKLSNIPLYYVNGKASTKSVLQIGSNA
                     DIIIGAAQSKSGPTNLFTGQIDEVQFYITPTLIQSGEFVEGPIANVTEAIPANVTEAI
                     PANVTEAIPANVTEAIPAN"
BASE COUNT         1686 a         1016 c          937 g         1721 t
ORIGIN      
        1 tgccatccaa tattacttca cagtgaagtc ttgatgaaat gttatcaaac tcgataaagc
       61 tgtcttctga tacaatcaat ggtcgtcttg ttatatcaag tgaatttaca gacacaattc
      121 caaaaacttt ggagtcttga aatatcatag gaccaccagc tgacattgaa tatgcagaag
      181 aaccaattgg tgtagagatt atgacaccat cgctgctgtc atgccaaact tcttcatcat
      241 taatcctcaa gacgtgttcc atcagcattg cactttttga tggaaatata gcaatatcat
      301 ttagtgctgg atagatgctt ttcccattga ttttgagacc aatgcgtgaa actttttcta
      361 ctgtaaaatc tttatttttt aaacggttaa caaaagaaga aaattctttt agatcaattt
      421 gagctaaaaa gccgcttgat tcagattcgt ttattccaag caccggagtt tcagaatcta
      481 aaatattgtg aaaataatgt ctaacccctt tgtcgccacc agtaacaaca acgcaatctg
      541 tatcttttat gacagtcttg ttaccaagaa gatacgagtc tataccgtgg ccttcaagaa
      601 ttgtcctgac agtctttact gcctgatcat tgtgctctgg gcttgcaatt gcaattctca
      661 tataatagaa atccactgat ttgtgcaata aaagcctaga tctgtgagaa tttcaccaga
      721 ttatacaggt aggaggcatt ggtctcaatt gcgcctcgtt gtggaatttt ttcaagaccg
      781 gtcttttttg tttcaagcgc tgcttgcgca gcaccaacag caaaacacat tgcccagaaa
      841 aagtcctttt ccttcaaata cgtacaagca aaagacgcac aaaaaatatc tccaattcca
      901 gtggtatcaa aaacttccgt gtttggaatt gttagataat acagcctgtc tttgacaagt
      961 actgtgattt cacgcttgtt tgttagtaaa acatgttcta tgcctttttt ttgtaaagcg
     1021 cgcattgcgt ccttgtctga ttgtccagtc agagcatatg cttcatcaac gtctactttg
     1081 attgcagata tgtttgagag attcatgtca gttttttcaa gatagatttt attgttctga
     1141 tcttttctgc gtaaaaatcc ctgcgggtca agaagtgtaa attttgaatc tttttttatt
     1201 ttttcaagtg tgtcgtatga tatttcatca aagacaggac ttacaatgca tccatctgta
     1261 tgcaatccat caaattctat aggatcacat tttgttttaa gatagaggtt tctgtcaatt
     1321 cctgaaattt caagcaaaaa tcttgtggtt gggttttgtg agaaagagat ttccggtatg
     1381 ataattttat ttttttcaag aaattctcta tgggtaaaat caggtccaat ttttgtatga
     1441 aactctacat cgaattttaa atttcttgca gtcagaccac aataacaggc tgggccgcca
     1501 gctgtttcta taactgaacc ttcacgcact atttcgtcaa tcgtgcagtg tgaaaatatg
     1561 tcaatcttca tcccatttct tgcaaaagtg gctcaacttt agtctttttt gagtcttttt
     1621 ttctcaaggc aggaaccgca gataatctgg cctttatcta tcatcacttc aacattggag
     1681 gcaagacaaa catgacacag acctcgcata tgatcaatag atcaaaaccg gcataaattc
     1741 cttaaagcaa gattataaag aggcatactg aagactaccc tatgccatta aaacgagcta
     1801 gccgtggacg acaaaaaggc ggcaagggat cagcaggtcg tattcaatgc acaaactgtg
     1861 gtgcaacagt accacgcgac aaggcaaaga aagtaacttc ccgtttgaat cttgttgagc
     1921 atacactagc aaaagagctt agagctcaag gggcatacat tgctgcgcca acagttctaa
     1981 aatggtactg tatttcttgt gcgattcact ttggaatatt aaagattaga tcagcagatt
     2041 ccagaagaaa tcacggtaga ctgcgataat cattctttta ttgcacgtgc agtagcaatt
     2101 attctctgaa ccaaagttat tcctgcaatt atcactacaa taattactgc atagtttaga
     2161 aatcctgcca taccaattat tgctataact aataatctct ctgctctttc gccaatacct
     2221 attccttgta actgcacacc aagtgattct gctctggctc tagtgtaact tacaagaagc
     2281 gagagagtta ttgctaaaag tacgacatat ccttccgcat atccaccaat taatattcca
     2341 agaaatattg ctacttcggc aatcttatca aatacagaat ccagaaatgc gccttttttt
     2401 gaggtctttt ttgtaatacg cgccacctgg ccgtcaacaa tgtcaaaaaa ccctgagacc
     2461 aaaagcatca ctcctccaac aacaaatgca tattcaaaat gccacgcata tgcgacagca
     2521 gacgcaaaag caaatgccaa tccaactgca gtccaaaagt tagcagacag gcctgttgct
     2581 gcaaacacta taccgatttt ttgcagagtc ggttttaggc tttctcggaa attgttaagc
     2641 atcctgtatc aactaattgt caataattta atcattaata acattgctac aaacgctatg
     2701 atatagtaga gattttctta tcaagatctt ctgccatatt ctcataattt tctctgcttt
     2761 tgccaatctc tttaagcttg gattcttcta ccatagtcag actctggttt acctgatcga
     2821 ctttatcctg caaagccatg tctgccatca tgaataatct attattttct ttccagagat
     2881 gctgcatcac atggttgatg tattcagtca tgtcaacaat taattgagtt gcattgccag
     2941 actgcagata atcttttgat gattcttcca ttctgtgtga gatctttctt gttatttcat
     3001 gttccattag catgactgca attggacctg cgttgcgtgg tattcccttt ttttcaagct
     3061 caggaaaaag cgagttttct tctttcccgt gatggcaaac atcagtaaaa ttctttgtaa
     3121 aatcaattac aggtccaaga attgattcag gaattgcttt tccttctttt agaagtgaaa
     3181 gtgctgccca cattgacttt agaacttttt caataagatc atgatctttt cgtagtgaag
     3241 ttgtagacaa tttgattcct aagattattt gcaagtatat ataatatcga gtttaattta
     3301 cggcgatggg cattatagca aagggtgcca agtgtaatgt aagtggatgt ggcaacgacg
     3361 gaattcgttc acttactaca tcaaaggtgg aaggaacagg ccttagcatt tctagcagct
     3421 caaaaaaaac tgtcctatgc aaagaacact acaaggaatg gaaaaaagct acaaagcaag
     3481 acagagatgt agaacgtgca agatatgaca gattctagca tttcttgcta aaatacacag
     3541 actatagatt gttgttattt agtttgcaac ttgtttaaca ggcattggca tattgtgaat
     3601 aatggttcac atagctaaca gcgcttattg taggatacat tatactttta ggatctgaac
     3661 tatgcttgaa acccaacgca tgcccaagct catgtttgag taacattgac aacgtgtcag
     3721 aatcatactg atgccatttt ccattgcaac tatatccaac accaatctcg attattttat
     3781 tatctataag atatccagca tgatatggat tagcttccct aatccattta atcacaatgg
     3841 ttgaatcatt ttttgatgtg gtggttttga attctgtgtt ggttgttttt ttccagaact
     3901 ggattgcatc tgaaagggag tctttggtgt ttgttgccca ctctggaatt ggttcaacat
     3961 acataaatcg cacagatgca attttatttt ttgataaatc atttgcagaa atttctgatg
     4021 gagttttttt ctgcaaaata tgtttattta tgtattgaat agcatcaatg tattctttat
     4081 cgctaatttt accttccatc caaaaaaatg aaattttttt ctgcaaatca gagatctgct
     4141 ctttcatagc aaacgaatca ttaaatgaca ttatgtttcc caataaaaat atcatgataa
     4201 gcgatgatag gagctttaca tgcatgctcc atgtccataa ttagcagaat taaactaatc
     4261 gaaatcctga cagatcgtaa aattatccaa tatacaacga atgaggcatg acctagtttt
     4321 tagcagtgaa aatcattcct gagaattatg attttggata ttaagcgagt taaaatcatc
     4381 atataatgag atgaaggatg cctattactg ataaagtatt caacatacat aatagaatca
     4441 attggaaaat gctttgtaaa cagagtggta gttattgaat aaattactat ttgcgtggtt
     4501 tgtttccata ctatgcatca tagcacttgg ctcattggaa aatgatgcca ttcaaacatt
     4561 ggtttatgca acgcatacag atgaaaacga gcgacaatat gaaacaaaac catctgacat
     4621 tttagatgaa attctgtcct ggtatttttt aaatctgaag atcaacacgc ttactatttt
     4681 tggagacata aagatgcaag acggttttat tggtaacact cttgatctag atggaatcaa
     4741 cgactatctt gtggttcaca gtactagtga tacaaatcaa ttcaagagta tgacattatc
     4801 tgcatgggta aaaccaaact atactagggc atcgcatcaa cttactgtaa ttagcaaaga
     4861 taaggcgttc gaagtttcca tcttaaatca aaaatcaaat tcatttgctc tattttccgt
     4921 atatgatgga atgaaatggg ttactgtaaa atcaaccagc cccattagtg aagaatggac
     4981 aaacattatt gcgacatact atggctctac actgtcaata tacgtaaatg gaaatcttga
     5041 agctaccaag aaattatcta atataccact ctattatgta aatggcaaag cgtcaacaaa
     5101 atcggtatta caaattggtt ctaatgcgga tattataatt ggcgccgcac aatcaaaatc
     5161 aggtcctacg aatttgttta ctggacaaat tgatgaagtg caattctaca ttacaccaac
     5221 tctaatacag agtggagaat ttgttgaggg gccaatagca aatgtgactg aagctattcc
     5281 tgcaaatgtg actgaagcta ttcctgcaaa tgtgactgaa gctattcctg caaatgtgac
     5341 tgaagctatt cctgcaaatg
//
LOCUS       DATIDL010000139         5603 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrosopumilaceae archaeon isolate SMAG_U16342
            k141_1783196, whole genome shotgun sequence.
ACCESSION   DATIDL010000139 DATIDL010000000
VERSION     DATIDL010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935836
            Sequence Read Archive: SRR25158326
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosopumilaceae archaeon (soil metagenome)
  ORGANISM  Nitrosopumilaceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrosopumilales;
            Nitrosopumilaceae.
REFERENCE   1  (bases 1 to 5603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/04/2024 05:09:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,520
            CDSs (total)                 :: 1,474
            Genes (coding)               :: 1,474
            CDSs (with protein)          :: 1,474
            Genes (RNA)                  :: 46
            rRNAs                        :: 1 (5S)
            complete rRNAs               :: 1 (5S)
            tRNAs                        :: 43
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5603
                     /organism="Nitrosopumilaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1783196"
                     /isolate="SMAG_U16342"
                     /isolation_source="soil"
                     /db_xref="taxon:2202732"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..460
                     /locus_tag="VLB45_06185"
     CDS             <1..460
                     /locus_tag="VLB45_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="collagen-like protein"
                     /protein_id="HSD05321.1"
                     /translation="PPGEKGLTGVPGQQGDRGPRGLAGPTGEQGPRGLQGAPGDKGPQ
                     GPQGPQGERGPHGPQGPPGERGPQGIQGMQGERGQPGPHGPIGEQGPRGQQGPPGEQG
                     PRGPPGPPGDKGVAGFSEQEKALVTQLLELLTAKNVLTTEQQIKLLSYLY"
     gene            500..784
                     /locus_tag="VLB45_06190"
     CDS             500..784
                     /locus_tag="VLB45_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007550195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05322.1"
                     /translation="MSQPKDKVDFKMLDHERVGNDFVSFKLEDGTIVKVKVDLDRVGI
                     ATNYKNPDGTPHYAINTSVKISVIPNDRKFTVEKNSVKAKSTSPPDQMFS"
     gene            complement(858..983)
                     /locus_tag="VLB45_06195"
     CDS             complement(858..983)
                     /locus_tag="VLB45_06195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05323.1"
                     /translation="MATKKRSKAKRKAPKRKAAKRRSAPKKAKRSGKKKAAKRRR"
     gene            complement(1101..1238)
                     /locus_tag="VLB45_06200"
     CDS             complement(1101..1238)
                     /locus_tag="VLB45_06200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05324.1"
                     /translation="MEKTKQEIIFMPEIPTYKRRRQYKCTKCGEKCWLQARRPSAEAV
                     Y"
     gene            complement(1314..2420)
                     /locus_tag="VLB45_06205"
     CDS             complement(1314..2420)
                     /locus_tag="VLB45_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HSD05325.1"
                     /translation="MGVPEKIQAIKDEMAKTQINKATEHHVGLLKAKLAKLKREQEEI
                     KSRSSGSSIGFDVKRTGDATVVFIGLPSVGKSTLLNRLTGAKSAVAAFQFTTTTVVPG
                     MMEHQGAKIQVLDLPGIIKGASTGKGLGKRVLAVAKSADLVLIILDVFQPYHEEIIRT
                     ELANIGIRLDQKPPNIVIEKTADGGISVSQQVPLTKMSISLLKDILRVYGVNNGRVLI
                     REDIDSEQLIDFISGNKTYVQSLTVMNKIDLVNQGFLNELQSNIKSKIIPISADSDIN
                     INALKDKIYEKLDFIRIYMRPKGGETDYDEPLITRNGSTVLEICNKLHRDMKRDFRYA
                     LVWGKSVKFGGQRVGLHHVLQDEDVLTILKTKGT"
     gene            complement(2431..2649)
                     /locus_tag="VLB45_06210"
     CDS             complement(2431..2649)
                     /locus_tag="VLB45_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010190810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF504 domain-containing protein"
                     /protein_id="HSD05326.1"
                     /translation="MSRKGRLSEIFSKAQYADDPTSYTVGYLDYDMIKEVTLPEFIKE
                     SDNFETIPITRIQFVKKGNRILFSKHTQ"
     gene            2732..3427
                     /locus_tag="VLB45_06215"
     CDS             2732..3427
                     /locus_tag="VLB45_06215"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HSD05327.1"
                     /translation="MQTKLFAIDIDGTITENGAGRIHLDALDALRHMVRLGHKVVFVS
                     GRSSVEGYVLSVFGGMTRVAVGENGGAITFGPSDHKLLGNKEECMKAFQLIKSIIPEA
                     NEKPVFPRLTEVVLERNFDIDIAKDIVAKNNLPVQLSDSQYAFHINSSGIDKARGLSE
                     VMKMFSMSAKDVIAIGDSETDVPLFKLAGLSIALGNAFDSVKSQATMSVSGKSGDGVI
                     QALEHISLKLARV"
     gene            3431..5317
                     /locus_tag="VLB45_06220"
     CDS             3431..5317
                     /locus_tag="VLB45_06220"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HSD05328.1"
                     /translation="MTLRVLLDEIRSNMSQICIKLNYPQTDFEISEASRPEFGDVSCN
                     VAFLLAKSLKKKPFDIAKTISEEYNAKLGKFVKQVTPHPSGYVNFFVNHSMLNNSTIS
                     ASLESNYGTINIGNNTKLVVEHTSVNPNKALHIGHVRNIIIGDTISRILQKAHYDVRV
                     LNYVDDSGLQVADIIVGFKYGGFSSEPPQDQKFDHYCGDVVYVQTTEKYETDTKLAEH
                     RIQVLKELEEGTSDTAKFANTITRRVLAEQLKTCWRLGVTYDCLNFESQIVRSNLWKT
                     VFEKMKSLQIIDLEKEGKNSGCWVIKSESDEEDKVLVRSNGIATYIAKDIPYAAWKLG
                     ILDDPFYYVPYGKQPGNRTLWETVLEKNANKKLDFAGDKVITVIDSRQSRLQKIITKI
                     MSSFKSQEGAYVHLGYESVTLSAETAKTLNVDTGGKQAQMSGRKGIYVNADNVLDILR
                     TKTYEEAKKRNTDLEQETLTDIAEKVAVGALRYAMIKQDLDKIITFDMTESLSLEGDT
                     GPYIQYAHARAARILEKASTKPNFDVSFDNLITEHEVNLIKSIGKFDIQIEDAAKNFS
                     PKIIARYCYDLAVAFNAFYEHVKVLTAENKSLVNARLCLVYCFKETLAKALDVLGIAS
                     PQRM"
     gene            5374..>5603
                     /locus_tag="VLB45_06225"
     CDS             5374..>5603
                     /locus_tag="VLB45_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA methyltransferase"
                     /protein_id="HSD05329.1"
                     /translation="MKKIKQNAYVLFFYNDSKKWLMKASRKQQFHTHVGVIDHKKVIG
                     KEYGSTITTNKGKYVYLLDPTIHDYVMKSQRST"
BASE COUNT         1783 a         1042 c         1099 g         1679 t
ORIGIN      
        1 tcctcccggt gaaaaaggtc taacaggtgt tccaggtcaa caaggtgaca gaggtcctag
       61 aggattagct ggcccgactg gtgagcaagg tccaagaggt cttcaaggtg ctccaggtga
      121 taaaggccca caaggtccac aaggtccgca aggagaacgt ggtccacacg gtccacaagg
      181 tccgcctggt gaaagaggac cgcaaggaat tcaaggaatg caaggagagc gaggtcaacc
      241 aggtccacac ggtccgatag gtgagcaagg tcctcgcggt caacaaggtc cgcctggtga
      301 gcaaggtcca cggggtcctc cgggtcctcc aggtgacaaa ggcgttgcag gattttctga
      361 acaagaaaag gcactggtaa cacaactgct tgagttgctt actgcaaaaa acgtactaac
      421 cacagagcag caaataaaat tactaagcta tctgtactag tcagcatact tcaaataact
      481 gcaaagcaat tttacgataa tgagccaacc aaaagacaaa gtagatttta aaatgctgga
      541 tcatgaaaga gtgggaaacg actttgtgtc cttcaaacta gaggatggta cgatagttaa
      601 agtcaaagtg gatttggatc gggtaggaat tgcaacaaat tacaaaaatc ctgatggtac
      661 accacattac gccattaaca cgtcagtaaa gatttcagta atcccaaatg acagaaaatt
      721 cactgtagaa aaaaattcag ttaaagccaa gtcgacttca ccgcctgacc agatgtttag
      781 ttgaataaat tattaataaa aatgtgaaag ttaccactta aaaaaccgcg ataactcttt
      841 gattttttag atcagaatta tcgtcgtcgc tttgcagctt tcttcttacc tgatctctta
      901 gctttttttg gagctgatct tcgctttgct gcttttctct ttggagcttt gcgcttagct
      961 ttagatcgct tttttgttgc cattgaaaaa tgtacaatca acttgctttt ctatagttaa
     1021 actaaacaaa attacactaa ctgatgcata atctatcaac attattttgg catcgatcta
     1081 atttttgatc gcaattttga tcaataaact gcttcagctg atggtcttct agcttgcaac
     1141 caacactttt ctccgcattt tgtacatttg tattgccttc ttcgtttgta agttggaatt
     1201 tctggcatga aaatgatttc ttgttttgtt ttttccatgc attttttgca ccaacgattt
     1261 tcaaaatcag cttccatgat tacaaaaaac aattcatgca gaaaagtgtt tacttaggtg
     1321 cccttggttt ttagtattgt aagtacatct tcatcttgta agacgtggtg taaaccaact
     1381 ctttggcctc caaacttgac actttttccc caaaccagcg catatctgaa atctcttttc
     1441 atgtctctgt gcagcttgtt acatatttct aaaaccgttg agccatttct tgttattagt
     1501 ggttcatcgt aatccgtctc gccacctttt ggtctcatgt atattctgat aaaatccaat
     1561 ttttcgtaaa tcttgtcttt gagtgcgttt atgttgatgt cagaatctgc ggatattgga
     1621 ataatttttg attttatgtt tgactggagt tcgtttagaa atccctgatt cactaaatct
     1681 attttgttca ttactgtaag tgattgcaca tatgttttgt tgccagaaat aaaatcgatt
     1741 aattgttccg agtcaatatc ttctctaatt aacacgcgtc cattgtttac gccatatact
     1801 cgaagtatgt cttttagaag actgatagac atttttgtaa gtggtacttg ttgactaaca
     1861 gatattccac catccgcagt tttttcaatt actatgtttg gtggtttttg atctagtctt
     1921 atgccaatgt tagcaagctc tgttctaata atttcttcat gatatggttg aaagacatct
     1981 agtataatta aaacaagatc tgcacttttt gctacagcta aaacacgttt tcccaatcct
     2041 tttccagttg atgcgccttt tattattcca ggtaaatcta atacttgaat ttttgcaccc
     2101 tgatgttcca tcattccagg aactacagta gttgtagtaa attgaaatgc agctacagct
     2161 gactttgcac cggtaagtcg atttagtaat gtagattttc caacacttgg taatccgata
     2221 aagactacag ttgcatcacc agttcgtttg acatcaaaac caatagagct tccagaagat
     2281 cttgatttga tttcttcttg ttcgcgtttt agttttgcaa gctttgcctt tagaagtcca
     2341 acatgatgtt ctgttgcctt gttgatctga gtttttgcca tttcatcttt tatggcttga
     2401 attttttctg gaactcccaa tatgactcac ttattgagta tgtttactga ataaaatcct
     2461 atttcccttc ttgacaaact ggattcttgt aattggtatt gtctcaaagt tgtcagattc
     2521 ctttatgaat tctggtaagg tgacttcttt tatcatgtca taatctaggt atcctaccgt
     2581 gtatgatgta ggatcgtcag catactgtgc cttgctaaaa atctctgata atcttccctt
     2641 tcgagacatg atttgattaa ttactgaaat gatattttag ctctgtctat ttatgagggt
     2701 cagttatatt ctcagtagaa tatacaagtt ggtgcaaaca aagctttttg caattgatat
     2761 agacggcaca attaccgaaa atggagcagg aaggatacat cttgatgcat tagatgcact
     2821 aagacacatg gtacgattag gtcataaggt agtttttgtg tcaggccgct cttcggtaga
     2881 agggtatgtt ctgtccgttt ttggcggtat gacacgcgta gcagtcggtg aaaatggcgg
     2941 tgccatcact tttggtccat ccgatcacaa acttttggga aacaaagaag aatgtatgaa
     3001 agcatttcaa ttgataaaat ccataatccc agaagcaaat gaaaaaccag tttttcctag
     3061 attgactgaa gttgtactag aaagaaactt tgacattgac attgcaaaag atatagttgc
     3121 aaaaaacaat ttgccagtcc aactttcaga tagccaatac gcttttcata taaattcaag
     3181 tgggattgac aaagcaagag gactctccga agtgatgaag atgttttcaa tgtctgcaaa
     3241 agatgttatc gctataggtg acagtgaaac agacgtaccg cttttcaagc tggctggtct
     3301 aagtattgca cttggcaacg catttgatag tgtaaaatca caggcaacca tgagtgtatc
     3361 tggcaaatca ggagatggcg tcattcaagc acttgagcac atctctctca agttggcaag
     3421 ggtgtaattt gtgacacttc gagtcctact agatgagatc agatcaaaca tgtctcagat
     3481 ttgtataaaa ctcaattatc cgcaaactga ttttgagata tctgaagctt cacgacctga
     3541 atttggcgat gtaagctgta atgtggcatt tttacttgca aagtcactaa agaaaaaacc
     3601 gtttgatatt gcaaagacaa tttctgaaga atataatgca aagttgggaa aatttgtaaa
     3661 acaagtaaca ccgcacccat caggatatgt gaattttttt gtaaaccatt ccatgttgaa
     3721 taattctacc atttctgcat cacttgaatc aaactatggc acaataaaca ttggaaataa
     3781 tactaaactt gtagtggagc acacaagtgt aaacccaaac aaggcactgc acatcgggca
     3841 tgtgagaaat atcatcatcg gtgatacaat atcaagaatc ttacaaaaag cgcactatga
     3901 tgtacgagta ctaaattatg tagatgattc tggtctgcaa gttgcagaca ttattgtggg
     3961 cttcaaatat ggtggatttt ctagtgaacc accacaagat caaaaatttg atcactattg
     4021 tggagatgtg gtgtatgtac aaaccacgga aaaatatgaa acagacacaa aacttgctga
     4081 acatagaatc caggtactca aagaactgga agaaggaaca tcagacactg caaaatttgc
     4141 aaatacaata acaaggcgcg ttcttgcaga acagctcaaa acctgctggc gacttggggt
     4201 gacctatgac tgcctaaact ttgaatctca aattgtacgc tctaatttgt ggaagaccgt
     4261 ctttgagaag atgaaatcat tgcaaataat agatcttgag aaagagggca aaaactcggg
     4321 ttgctgggtc atcaagtctg aaagtgatga ggaggacaag gtgctggtaa gaagtaatgg
     4381 gattgcgacc tacattgcaa aggacatacc atacgctgca tggaagcttg gcattctaga
     4441 tgatccattt tactatgtcc cgtatggcaa gcaaccagga aaccggactt tgtgggaaac
     4501 ggtactggaa aaaaatgcca acaagaaact tgactttgca ggcgataaag taataacagt
     4561 aattgattca cgccagtctc gtttacaaaa aataatcaca aaaataatgt ctagtttcaa
     4621 atcgcaagaa ggtgcatacg ttcatcttgg ttatgaatct gtgaccctga gtgcagaaac
     4681 cgcaaagaca ctcaatgttg atactggagg aaaacaagct cagatgtctg gacgtaaagg
     4741 gatctatgtc aacgcagata atgttcttga catcttaaga acaaaaacat acgaagaagc
     4801 aaagaaaaga aatactgatc ttgaacaaga aactcttact gacattgcag aaaaagttgc
     4861 cgttggtgca ttgcgatatg caatgataaa acaagatttg gataaaatca taacatttga
     4921 catgacagaa tccctaagtc ttgaaggcga tacgggacct tacatacagt atgcacatgc
     4981 aagagccgca agaattctag aaaaagctag cacaaaaccg aattttgacg tatcgtttga
     5041 taatcttatt acagaacacg aggtaaactt aatcaaatcg atagggaaat ttgatatcca
     5101 gattgaagac gcggcaaaga atttctcgcc aaaaataatt gcgcgttact gctatgatct
     5161 ggcagttgca tttaacgcat tttatgagca tgtcaaagtt ctaacagcag aaaacaagtc
     5221 gttagtaaat gcaagactct gtcttgttta ttgctttaaa gaaactctgg ccaaggcact
     5281 agatgtgctt ggaattgctt cgccgcaaag aatgtaatac ttgagtaatc aaaagaggta
     5341 aattcttatc cacattagcc aagctctatt tctgtgaaaa aaataaagca aaatgcttat
     5401 gttctgtttt tttacaatga ctcaaaaaaa tggttaatga aagcatcaag aaaacaacag
     5461 tttcatacgc acgtgggagt aatcgatcac aaaaaagtaa taggaaaaga gtatggttcc
     5521 acgataacta ccaacaaagg caagtatgtc tatcttttag atcctaccat tcatgattat
     5581 gtgatgaaaa gccaacgcag tac
//
LOCUS       DATIDL010000140        13568 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrosopumilaceae archaeon isolate SMAG_U16342
            k141_1785434, whole genome shotgun sequence.
ACCESSION   DATIDL010000140 DATIDL010000000
VERSION     DATIDL010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935836
            Sequence Read Archive: SRR25158326
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosopumilaceae archaeon (soil metagenome)
  ORGANISM  Nitrosopumilaceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrosopumilales;
            Nitrosopumilaceae.
REFERENCE   1  (bases 1 to 13568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/04/2024 05:09:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,520
            CDSs (total)                 :: 1,474
            Genes (coding)               :: 1,474
            CDSs (with protein)          :: 1,474
            Genes (RNA)                  :: 46
            rRNAs                        :: 1 (5S)
            complete rRNAs               :: 1 (5S)
            tRNAs                        :: 43
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13568
                     /organism="Nitrosopumilaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1785434"
                     /isolate="SMAG_U16342"
                     /isolation_source="soil"
                     /db_xref="taxon:2202732"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2643)
                     /locus_tag="VLB45_06230"
     CDS             complement(<1..2643)
                     /locus_tag="VLB45_06230"
                     /inference="COORDINATES: protein motif:HMM:NF014544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HYR domain-containing protein"
                     /protein_id="HSD05330.1"
                     /translation="MKRLPLVVSGIVAILLASTFSQYSIAETAPVFVKQWGSQGLQTT
                     GLFSFPQNSDVDSSGNIYVTDYGNRRIQKFDSDGNFLHTWGIKGSDNGQFQFPIGIAI
                     QGEYVYVVDNELNRVQKFDTVGKYITQWGVKGSETGKFLLPQGIAVDPNGDVYVADTG
                     NNRIQKFSADGKFLLAWGSSGLGNGQFLNLHGIAADSQGSIYVSDSGNNRIQKFTTDG
                     IFIKSFDVSSGSSLKFPEGLDVDSSGNIYVADTGNNRMVKIDRDGNSLFSWGSSGQTS
                     GNFNNPKDIVADSNGNVFVVDSSNHRIQKFASTTSTPVTETIDPVTETIDPVTETITP
                     VLNPLPNDKTKPVISAPANVIAEANGILSTVLIGQATATDASGIASITNNAPTLFPIG
                     DTIVTWTAIDVAGNSATTKQVVTVQDTTKPKITTPVDVVSEAVNPIGNIVSLGDATVI
                     DNVEISSITNDAPSSFPLGTTTVTWIAVDTAGNTATATQKIKITDTTAPVIIPPSDVV
                     FEAMSSTDNIVQLGDVNATDVQSVTITNDAPAVFPIGQTLVTWTATDAAGNSAFVTQM
                     VTVSDTLPPTLVLPADVVAEATSHDHNIISIGQATTEDLIGVSSITNNAPAEFGFGQT
                     IVTWTAVDTFGNTISANQTITIIDTTSPQLAVPKDIIAEATDPTSNIIDVGNADATDI
                     IGIASVTNNAPDSFPIGITPVTWVAIDTSGNSVNATQTVTINDTTSPVVVVPADVIVE
                     ATSSSGSVISIGNADATDITGISSITNDAPATFPVGETIVTWTATDNSGNSANATQKI
                     TVVDTTSPTLSVPEDVIFEALAPIGNSISIGDASADDLVGVVTITNDAPSSFPLGETI
                     VTWTATDAAGNSATAIQKVTVVDTT"
     gene            complement(2720..3496)
                     /locus_tag="VLB45_06235"
     CDS             complement(2720..3496)
                     /locus_tag="VLB45_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSD05331.1"
                     /translation="MIISDTYSIFWREMKRYRKSRSGVLIRLIQPAIWIIVIGNTFSG
                     TQPLIQSVGYDLSYIEFMAPGVIILTAIFTSIFGGVNTLWDRRYGFMNKALVSPISRS
                     SIAIGKMLAISLISALQSSLILGIALAIGVQFHNPLMFIPIMLIVIAFSLGFSGISVV
                     VAATAKSQETFWGIINFLGMPLFMLSPALFPLELLPDWLAFVAKLNPVTYSVLLIREM
                     MTDTVQFASVAMEIAIISGFVVIMVALASYVFTREVNKPF"
     gene            complement(3493..4494)
                     /locus_tag="VLB45_06240"
     CDS             complement(3493..4494)
                     /locus_tag="VLB45_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016939605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HSD05332.1"
                     /translation="MYSVETKSLSKTYGTGIKAVDEISITVNDGEIFGFLGPNGAGKS
                     TTIMILTTLLKPTSGTALVAGFDVVTQAKKVRENIGYVQQDSTVDEYLTGRENLILQA
                     KLNHIEKNSIQNRVDEILDLVELSDRQHDSVISYSGGMRKRLDIGGGLLHRPKVLFLD
                     EPTLGLDIQTRYKIWEYIKKIHKEFGMSIFLTTHYMEEADRLCDKISIIDYGKIKITD
                     SPQSMKSALGSDVVVFSVEPGIDLENLFHEIKKNPLVKDLKADGDEVTVFSSNGNQFM
                     PKMFEILSSLGIKIESISLTKPSLDDVFLSHTGRELRDENGGYNRKKEQEKMRRLRA"
     gene            complement(4552..5004)
                     /locus_tag="VLB45_06245"
     CDS             complement(4552..5004)
                     /locus_tag="VLB45_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HSD05333.1"
                     /translation="MATNLKEVPGNLSIADEFCGKKVVDREGIQYGKIKHLHINPETL
                     EVSGITVHQGFHKDYYLSRDYIDRFTEESVLLGTPPLRKDVVVVDIDGHKIGKIKKLN
                     RNQDTNELESIEVSEGLMHSRVFGKSEIWGVGEKVILRLAKEEYKKSN"
     gene            5092..5412
                     /locus_tag="VLB45_06250"
     CDS             5092..5412
                     /locus_tag="VLB45_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010193593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05334.1"
                     /translation="MNLEDGQVFRATLVTRGRKTGRQHSVELRAVFYNKKVYFSRRNP
                     NSDWLKNAITNKEVKIEYGDAVLSGIASLVTDDNLAKKISRLKYSDKRSEESRIVLQV
                     TLCE"
     gene            complement(5396..6040)
                     /locus_tag="VLB45_06255"
     CDS             complement(5396..6040)
                     /locus_tag="VLB45_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007549878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05335.1"
                     /translation="MLPLDKDGIPYDEGSERTSNVDDYKITCIQFIKDISNDYLAWVD
                     YYKLPPDEDKKRRDRIFATVERTNEWIAKVATTIKGVDVVMDDGIQKMAESTAGYPFQ
                     IGVFVNKTSGYTVAKPGIIDINIKSIGRDGRKTNLGWHQKETRFLIKSTAKDISIDES
                     SITEAEYDMLDLHLNMNAQGCQSRDIISFTVIVSEVLDGQEYERRGVTTIIHIT"
     gene            6114..6620
                     /locus_tag="VLB45_06260"
     CDS             6114..6620
                     /locus_tag="VLB45_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05336.1"
                     /translation="MSFSQMVEELQRELKSNMPQIRILLMKSPAIAFTTINEIATSIG
                     KKYKMTLLLNFPQREKINDFESYGTENISMIIDRNKKIFPIKREMIKLKALEILGNAK
                     VEDAYMYEGKEGVKVFFDSGRIDILPHSLHLWCKFDQSIARYCDWLFTNCYYPKPGVE
                     GAVSEVSS"
     gene            complement(6572..7255)
                     /locus_tag="VLB45_06265"
     CDS             complement(6572..7255)
                     /locus_tag="VLB45_06265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05337.1"
                     /translation="MANKVKLIALFVMIAAGIIGLLWLPLTTGLPGQDENIIPREASK
                     WHMGKGIEYDPVMNYDIYTDKMQFSTKIEFAGSGFEKYMMISIVDSKLGKDLIQKIPI
                     GNAYVFGEVSDEARPYTTILDQTVFSIRDIALEDKYLVKQAVWDTVFVGATTRDITIT
                     EHGKTDFRFGSVSAFTASYDIGDKQSKFWIVDNLPLPVKSEIYDIEGNLQYSYELTSL
                     TAPSTPGFG"
     gene            complement(7275..8822)
                     /locus_tag="VLB45_06270"
     CDS             complement(7275..8822)
                     /locus_tag="VLB45_06270"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HSD05338.1"
                     /translation="MKKYLAIAFFVVLLSASIGIKESFAADTWYVGEGLKEGDYFRYT
                     LCQVDYKSCTEFEMDFWVNGKTEHGDWNLQVVVIDGNKIVTDNMEVGRAAPEPVNVSK
                     ELLPYASAYKSSIVWLSGFATRNDPKDFSAPAWGKIGSIGGMQVGPTGAKETVTVPAG
                     TFETFVVGWHKSTDNKIWVKDNFPFPIKALTFVDVTAGTIPVQYQFELLEYGNTANPP
                     DFLDAVSTPTTPEGQCPRDDSIVRKQANTDTNKMIIEYSYSPEHPKTGCEIKWNLDFA
                     KSFDVNQFESDVHYDVFVVDDDGVPVRSHSNDQGRDHLFSPAGKELFSMVVKEPEGIA
                     HYMIHVYGTGPDGVAPNTEKAGSLTIDIEIAKGTTTSPKPPVKESNPPMEIAIDIPEG
                     AADINGEIFYDPADTYVTPGSTITWTNSDTAAHTVTSGTPTDGPDGKFDSGLFGPGKT
                     FETILDEKGTHPYFCQVHPWMVGSITVDPTAVPEFPVSVIMIMILVVAVTVVISRFRT
                     NVFNLKF"
     gene            8898..9068
                     /locus_tag="VLB45_06275"
     CDS             8898..9068
                     /locus_tag="VLB45_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016939603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05339.1"
                     /translation="MCECDKVHMYEVEFKLDGMTVVPTHKNCGDRLNEKQVDKFQKEL
                     VKSWGFEQEKEK"
     gene            complement(9328..10206)
                     /locus_tag="VLB45_06280"
     CDS             complement(9328..10206)
                     /locus_tag="VLB45_06280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05340.1"
                     /translation="MNLKNIVEEFSLSDYPVALGGCRSDGTNFECCEYNITVFDEKNH
                     ESVHEIGGEIIKLHHAKFFETNSGILNQFQNLTIISDDQWKLRMFLSEIKEKRAKISN
                     SYIQSCLVDAGVYATKAKDAVKTSDALAHLWLKCGACFLADAVLLLNSKRPSPTHMLE
                     LIRGFKKNKTNESFSIVHQCLGIERATTSLLSRMIKSTIGFSDIVESNGHSKIIQRKY
                     DHLVHHSLLSDCYFYLCYTNRNNIIKVKDILHRNLEFMHILRVALDIENDPLAIDRQA
                     SNLLKTTNDLLSTIKS"
     gene            10316..10391
                     /locus_tag="VLB45_06285"
     tRNA            10316..10391
                     /locus_tag="VLB45_06285"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10352..10354,aa:Glu,seq:ctc)
     gene            10463..10633
                     /locus_tag="VLB45_06290"
     CDS             10463..10633
                     /locus_tag="VLB45_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05341.1"
                     /translation="MVGPKDGGFGFDGAPKYSELENKSTICVQCQAGQHGKCLAKTNQ
                     QLQCDCELCMLS"
     gene            complement(10637..11179)
                     /locus_tag="VLB45_06295"
     CDS             complement(10637..11179)
                     /locus_tag="VLB45_06295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05342.1"
                     /translation="MDCGLKTEEQFHELCLRVIPTRNGMKMNVVTQNQIDHVKMIRNM
                     IVNKSPLQDALLIFSIAGFFVLGFAAGGYLEMFGSDIGNAKTMDSASLGFIQHQNSPS
                     RIIYENCLGYGNAQSIYVKCPDGMGSVYNAMLDMPYNLEKKFSKDTFSIRGISLGENF
                     DGSLELGYQNNWYPTSLSIQ"
     gene            complement(11635..12204)
                     /gene="endA"
                     /locus_tag="VLB45_06300"
     CDS             complement(11635..12204)
                     /gene="endA"
                     /locus_tag="VLB45_06300"
                     /EC_number="4.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000214 - tRNA-intron endonuclease
                     complex [Evidence IEA];
                     GO_function: GO:0000213 - tRNA-intron endonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006388 - tRNA splicing, via
                     endonucleolytic cleavage and ligation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-intron lyase"
                     /protein_id="HSD05343.1"
                     /translation="MSDIEFKVEGVLLKDHVLISDKNMQHSLEQKGFGEIVKKKFLLK
                     PFESLYLLYTDKLKLTRGTQQIPFDFMMQECIKFDNDILTKFLIYRDLRTRGYIAKDG
                     FGFGSDFRVYERGQFGEKAAKYVIFGLSEGRREKMGLLQKQIEQITKMGKEPVIAVIE
                     RRGEVIYYKISRIKFLENKDHTDLSPVEF"
     gene            12378..12884
                     /locus_tag="VLB45_06305"
     CDS             12378..12884
                     /locus_tag="VLB45_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HSD05344.1"
                     /translation="MHGANYRIGNGQPYTRKKYIKGKPQIKIAKFSSGKRDDYDYCVQ
                     LCSSEKVQIRHMAIESARLSANKAIETVAGETGYFSILRVYPHILLRENKMIATAGAD
                     RLQEGMRGAFGKAVSLAARVDRGQCIMEACVKKEHLEVAKKALHGAAVKLPMTPTIKV
                     IPLKAALA"
     gene            complement(12876..13151)
                     /locus_tag="VLB45_06310"
     CDS             complement(12876..13151)
                     /locus_tag="VLB45_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05345.1"
                     /translation="MDPKLEEKINQKIKEVAENSDEIFKIINSLEKLRTHSDSFGYGI
                     VIGRLYNSFYYQCRRILKRNPTEEEFMEFLSILNKRQTEIRDTLKLG"
     gene            13234..13307
                     /locus_tag="VLB45_06315"
     tRNA            13234..13307
                     /locus_tag="VLB45_06315"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13269..13271,aa:Gly,seq:gcc)
     gene            complement(13312..>13568)
                     /locus_tag="VLB45_06320"
     CDS             complement(13312..>13568)
                     /locus_tag="VLB45_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007549863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05346.1"
                     /translation="ILQDGLGHVFVEISLNFSYGGISEKILLYANESIEFFESLAATS
                     MLALSSPHSEYGRSNVFMIQLPKPERAVNALEVIKNGLKK"
BASE COUNT         4117 a         2586 c         2437 g         4428 t
ORIGIN      
        1 agtagtatca actacagtaa ccttttgaat tgcagtagca gagtttcctg ctgcatcagt
       61 tgcagtccat gtcactatgg tttctccaag cgggaaagat gatggagcat cattggttat
      121 tgttactact cctacaagat catcagcaga tgcatcgcca atagatattg aatttcctat
      181 tggagctaat gcttcaaaga tcacgtcttc aggtactgag agcgttgggg atgtggtatc
      241 aactactgta atcttttgag ttgcgttagc agaatttcct gaattatcag ttgcagtcca
      301 tgtcactatt gtttctccta ctggaaatgt tgcaggagcg tcgtttgtta ttgaggatat
      361 acctgttata tcagtcgcat ctgcgttgcc tatagatatt acacttccac ttgagcttgt
      421 agcttctaca ataacatcag caggaactac aacaactgga cttgtggtat catttattgt
      481 gacagtttga gtagcattaa cagaatttcc tgaagtatct attgctaccc aggtaacagg
      541 tgttattcca attggaaatg aatctggagc attatttgtt acagatgcaa ttccaataat
      601 gtcagtggca tcagcatttc caacatctat tatgtttgaa gttggatctg tggcttctgc
      661 aatgatgtct tttggtacag caagttgcgg tgaagttgta tcaattattg ttattgtctg
      721 atttgcagaa attgtgtttc caaaagtatc gactgcagtc caagtcacta tggtttgacc
      781 aaaaccaaat tcagcagggg cattgtttgt aatagaagag actccaatca aatcctctgt
      841 tgtcgcttgt ccaattgata ttatgttatg atcatgactt gttgcctctg caacaacatc
      901 tgcaggaaga acaagagtcg gtggaagtgt gtcagataca gtaaccattt gtgttacaaa
      961 agcagaattt cctgctgcat cagttgcagt ccaggttact agggtttggc caatgggaaa
     1021 aacagctggt gcatcatttg ttattgtaac agactgtaca tcagttgcat ttacgtctcc
     1081 taattgtaca atattatcag tagatgacat ggcctcaaaa acaacatccg atggaggaat
     1141 tatcacagga gcagtagtat cagttatttt gattttttgc gtggcagttg cggtattccc
     1201 tgcagtatca acagcaatcc aagtcactgt agttgtacca agtgggaaag atgatggagc
     1261 atcgtttgtt attgatgaaa tttctacgtt gtctattacc gtagcgtcac ctaatgatac
     1321 gatgttacct attggattta cagcttcaga taccacatct acaggtgtag ttatctttgg
     1381 ttttgttgtg tcttgaactg ttacaacttg ttttgttgta gcgctatttc ctgccacatc
     1441 aattgcagtc catgtcacta tggtatcgcc aattggaaat aatgtcggag cattgtttgt
     1501 aattgatgct attccacttg catcagtagc agttgcctga cctatgagaa cggtgcttag
     1561 aatcccattg gcttctgcta tcacatttgc tggtgccgag attactggtt tggttttgtc
     1621 atttggaagt ggatttagaa caggggttat tgtttcagtt actggatcta ttgtttcagt
     1681 tactggatct attgtttctg ttactggagt tgatgtcgtt gaggcaaact tttggattct
     1741 gtgattgctg ctgtcaacta caaacacatt tccgtttgaa tccgctacaa tatcttttgg
     1801 attattaaaa ttaccacttg tttgacctga acttccccac gaaaacagtg aatttccatc
     1861 tctatcgatt tttaccattc tgttgttacc agtgtctgcc acgtagatat taccagatga
     1921 atcaacatcc aatccctccg ggaatttcaa gctgctgcct gatgaaacat caaatgattt
     1981 tatgaaaatt ccatctgtag tgaatttttg aatcctgttg ttgccagagt cagatacgta
     2041 gatactccct tgcgaatctg ctgcaatgcc atgcagatta agaaattgtc catttccaag
     2101 tccgctagat ccccatgcca acaagaattt tccatcggct gagaattttt gtattctatt
     2161 atttcctgta tcagcaacat atacatcacc gttaggatcc actgcaattc cctgtggcaa
     2221 aagaaacttg ccagtttccg agcccttgac tccccattgg gttatgtatt ttccaacggt
     2281 gtcaaacttt tgtactcggt ttagttcatt gtcaacaaca taaacatact caccttgaat
     2341 tgcaattcca attggaaact ggaactgccc attatccgaa cctttgattc cccacgtatg
     2401 caagaaattt ccatcactat caaacttttg aattcttcta tttccataat cagtgacata
     2461 gatatttcca gatgaatcta catctgagtt ctgtggaaat gaaaataacc cagtagtttg
     2521 cagtccttgc gaaccccatt gttttacaaa aacgggagct gtttcagcaa tagaatattg
     2581 agaaaatgta ctggccagta agatagctac aatcccagat accactagtg ggagcctttt
     2641 caactaccaa ctcttgtaaa atactaaaaa gatctccaaa gttaggaatt gttgcagttt
     2701 ttgtcaattt tcggtcggct tagaagggtt tgttgacttc cctagtaaag acatagcttg
     2761 ccagagcgac cattatgacc acaaatccag aaatgattgc aatttccatt gcaaccgaag
     2821 caaactgaac cgtatctgtc atcatttccc ttatcaaaag aacagaatag gtcacagggt
     2881 ttagtttggc aacaaaggca agccaatctg gcaacaattc cagtggaaat agagctggac
     2941 ttagcataaa gagcggcatt ccaagaaagt tgataattcc ccaaaacgtt tcttgtgatt
     3001 ttgcggtagc tgcaaccact acggaaatgc cggaaaaccc aagcgaaaaa gcaatgacaa
     3061 taagcattat tggaatgaac atcagaggat tatgaaactg gacaccgatt gcaagtgcga
     3121 ttcccagaat gagacttgat tgtaatgcag aaatcaaaga tattgcaagc attttcccta
     3181 tcgcaattga tgatctagaa attggagaca ccagagcctt gttcataaac ccatatcgcc
     3241 tatcccacaa agtgttgact ccaccaaata tgctagtaaa aatcgcagtg agtattatca
     3301 caccaggtgc catgaattct atgtaagaca gatcatatcc tactgattgt atcagtggtt
     3361 gtgttccaga aaatgtattt ccaataacta tgatccaaat tgcaggctga atcaacctga
     3421 tgagtacacc gcttctggac ttgcgatatc ttttcatctc tctccagaaa attgaatatg
     3481 tatcagaaat tatcaagccc ttagtctcct cattttttct tgttcttttt ttctgttata
     3541 tccaccattt tcatcgcgta gctctcttcc agtgtgtgaa agaaagacgt catcaagact
     3601 tggtttggta agtgatattg attctatttt gattccaaga ctggacaaaa tttcaaacat
     3661 ttttggcatg aactggttac catttgatga aaaaacagta acctcatctc catctgcttt
     3721 taggtctttc acaagaggat tttttttaat ttcatgaaac aagttttcca agtctatgcc
     3781 aggctcaact gaaaatacaa caacatcact cccaagggca cttttcatag attgagggga
     3841 atcagttatc ttgatttttc catagtcaat tatcgatatt ttatcacaaa gtctgtctgc
     3901 ctcttccatg taatgggtag taaggaatat ggacatcccg aattctttgt gtattttttt
     3961 tatatattcc cagattttgt atcttgtttg aatatctaat cctagtgtgg gctcatccag
     4021 aaacaggact ttgggcctgt gcaaaagccc acctcctata tcaagcctct ttcgcattcc
     4081 accagaatag gaaattacag aatcatgttg tcggtctgaa agctctacca gatctaagat
     4141 ttcgtccact ctgttttgta tagagttttt ttcaatatga tttagtttag cctgaagtat
     4201 gagattttct cttcctgtaa gatattcatc tactgtagaa tcttgttgca cataaccaat
     4261 gttttctctt acttttttgg cctgggtcac aacatcaaat cctgccacaa gtgcagtgcc
     4321 agatgttggt tttaacaacg tggtcaagat cataatggtt gtactttttc cagccccatt
     4381 tggtcccaaa aaaccaaaga tctccccatc atttactgtg attgatattt catcaactgc
     4441 ttttattcca gttccataag tttttgaaag agattttgtt tctacagaat acaataagaa
     4501 gtggacaatt tgtgcattat aaattgttaa gcaaaaaaga aatgcgtgag atcagttgga
     4561 ttttttatat tcttcttttg ctagtcgtag tatgactttt tcgcctacac cccaaatttc
     4621 tgattttcca aaaactctgg agtgcatcag tccttctgac acttcgattg attcaagttc
     4681 attggtgtcc tgatttcgat ttagtttttt tatcttgcca attttgtgac cgtcaatgtc
     4741 tactacaaca acatcttttc taagtggcgg cgtgcctagc aaaacagatt cttcagtgaa
     4801 tctgtcaatg taatcacgtg ataaatagta gtctttatga aatccttggt gcactgtgat
     4861 tccagaaact tcgagcgtct cggggtttat gtgtagatgc tttattttcc catactgaat
     4921 tccttctcta tcgactacct tttttccaca aaattcatcg gcgatactga gatttcctgg
     4981 aacttctttg agattggttg ccatgtatag aaataagaga tttagtttat tgaatcagat
     5041 atcagagttt tcaatccaag gggttaaatt ctttataaca aaacaaggct catgaatttg
     5101 gaagacggtc aggtgtttag agcaacgctt gtcacgaggg gcagaaaaac aggtagacaa
     5161 cattcagtag agcttagagc agtattttat aacaaaaaag tgtatttttc aagaagaaat
     5221 ccaaacagtg attggctcaa aaatgcaatt acaaacaagg aggtaaaaat agaatatggt
     5281 gatgcggttt tgtctggaat tgcatctctt gttacagatg acaatcttgc aaaaaaaata
     5341 tcgcgtttaa agtattccga taagaggtcg gaagagtcta gaattgtttt gcaagtcacg
     5401 ttatgtgaat gattgtggtt accccacgcc tttcgtattc ttgcccatct aaaacttctg
     5461 acactataac agtaaaggaa ataatatctc tagactgaca tccttgggca ttcatgttaa
     5521 gatgtaagtc aagcatatcg tactctgctt cagttatgct tgattcatca atcgatatgt
     5581 ccttggcagt ggattttatc aaaaagcgtg tttctttttg atgccagcca agattggtct
     5641 ttcgaccatc acgacctatc gactttatgt taatgtcaat gattcctggt tttgcaacgg
     5701 tatagcctga ggttttattt acaaagacac ctatctgaaa aggatagcct gcagtgcttt
     5761 ctgccatttt ttggataccg tcatccatca caacatcaac tccttttatt gtggtagcca
     5821 cttttgctat ccactcgttt gttctttcca cagtagcaaa aattctgtca cgtctttttt
     5881 tgtcctcgtc tggtggaagt ttgtaataat ccacccaagc aagatagtcg ttgctaatgt
     5941 cctttatgaa ctggatacag gtaattttgt aatcatcaac attacttgtt ctttctgagc
     6001 cttcatcata tggaatgcca tctttgtcta gtggtagcat gttgcctaat aatcagagat
     6061 gtaaaaaaag gtataaccta atagatatat tccaaaaatt tgactttgag atcatgagtt
     6121 tcagccagat ggtagaagaa ttacaacgtg agctaaaatc aaacatgcca caaatcagaa
     6181 ttcttttgat gaaatcacca gccattgctt ttacaacaat taatgaaatt gctacaagta
     6241 taggaaaaaa atacaagatg actttgctgc taaattttcc acaaagagaa aaaatcaatg
     6301 attttgaatc ctatggaact gaaaacatca gcatgatcat tgacaggaac aaaaaaatat
     6361 ttcctattaa aagagaaatg ataaaattaa aagcattaga aattttaggt aacgctaaag
     6421 ttgaagacgc atacatgtat gagggaaaag agggagtcaa ggtctttttt gattctggca
     6481 ggatagacat tcttccccat tcgcttcact tgtggtgcaa gtttgatcaa agtatagcaa
     6541 gatattgtga ttggcttttt acaaactgct attatccaaa gcctggtgtt gaaggagcag
     6601 taagcgaagt aagctcataa gaatactgca gatttccttc aatatcataa atttcagatt
     6661 ttacaggcaa cggcaagttg tcaacaatcc agaatttgct ttgtttatca ccaatatcat
     6721 aagatgctgt aaaagcagat actgaaccaa acctaaagtc agtcttgcca tgttctgtta
     6781 tggtaatatc cctagttgta gcgccgacaa atactgtgtc ccataccgct tgttttacga
     6841 ggtacttgtc ttcaagtgca atatccctta ttgaaaaaac tgtctgatct agaattgtgg
     6901 tatacggcct tgcttcgtct gagacttcac cgaaaacata tgcattacca attggaattt
     6961 tttgtatcag atcttttccc agtttagaat caacaatgct aatcatcata tatttttcaa
     7021 aaccactgcc agcaaattct attttggtag agaactgcat cttgtcagta tagatgtcat
     7081 aattcataac aggatcatat tctatgcctt ttcccatgtg ccatttgctt gcctcccttg
     7141 gaataatatt ttcatcttgc cctggaagtc ctgtagtgag tggaagccag aggagtccta
     7201 tgatacctgc cgctatcata acaaataatg caattagctt taccttgttt gccatttgtt
     7261 tagtattata cgctctaaaa tttaagattg aatacgttag ttctaaatct cgagatcaca
     7321 actgtcacag caacaaccaa aatcattatc ataattacac tgactggaaa ttctggtact
     7381 gctgtaggat caactgttat agatccaacc atccacgggt gtacttgaca aaagtatgga
     7441 tgtgttcctt tttcgtctaa aatagtttca aatgtttttc ctggaccaaa aagaccgctg
     7501 tcaaactttc catctggtcc atcagttgga gttccacttg tgacagtatg tgccgcagta
     7561 tcactattag tccaagttat tgttgaacca ggtgtaacat atgtatccgc aggatcgtag
     7621 aaaatttcgc cattaatatc agccgctcct tctggtatgt caatcgcaat ttccatcgga
     7681 ggattagatt ctttcactgg tggttttgga gatgtagttg taccctttgc aatctcaata
     7741 tcaattgtaa ggctaccagc tttttcagta tttggtgcca caccatctgg gccagtacca
     7801 taaacatgaa tcatgtaatg ggcaattcct tcaggttctt ttacaaccat agaaaacaac
     7861 tcttttcctg caggggagaa caaatgatct ctaccttgat cattcgaatg agatctcaca
     7921 ggaacaccgt cgtcatctac gacaaagacg tcgtaatgaa catctgactc aaactggttg
     7981 acgtcaaatg actttgcaaa atcaaggttc catttaattt cacatcctgt tttcggatgt
     8041 tctggagaat aactgtattc tataatcatt ttatttgtgt cagtgtttgc ctgtttgcgt
     8101 actatagaat cgtcacgtgg acattgtcct tctggtgttg ttggagtgga aacagcatct
     8161 aaaaagtcag gaggatttgc agtatttcca tattctaaca attcaaattg atactgaact
     8221 ggtatggttc ctgctgttac atcaacaaat gtgagtgctt taatcgggaa tggaaagtta
     8281 tcttttaccc atatcttgtt gtctgtagac ttgtgccaac caacaacaaa tgtttcaaag
     8341 gtgccagctg gaacagttac tgtttcttta gcacctgttg gtcccacttg cataccacca
     8401 atagaaccaa ttttacccca agccggagca ctaaagtctt ttggatcatt cctggttgca
     8461 aatccagaaa gccacactat ggatgatttg taggccgaag cataaggaag tagttcttta
     8521 cttacattca caggttctgg agctgctctg cccacttcca tgttatctgt tactattttg
     8581 ttgccatcga taacaactac ttgtaaattc caatcaccgt gttctgtctt gccgtttacc
     8641 caaaaatcca tctcaaattc tgtacatgat ttgtaatcaa cttgacagag ggtgtatcta
     8701 aaataatccc cttcttttag tccttcacca acataccaag tgtcagctgc aaaagattct
     8761 ttgattccaa ttgaagcaga taatagaact acgaaaaaag caatagctaa atactttttc
     8821 aagcagtttc ataagaaaaa atcccatagt taaatctttc aaatacgcaa atatagtggc
     8881 agaatattat ttcgaacatg tgtgaatgcg acaaagtaca catgtatgaa gttgagttca
     8941 agcttgatgg tatgacagta gttcctacac acaaaaattg cggagatcgg ttaaacgaaa
     9001 aacaggtaga caaattccaa aaagaattgg taaaatcttg gggcttcgag caagaaaaag
     9061 aaaaataatt tagagatatt aaaaaagaaa ggaaaaggag cccgcgagaa tctatttgta
     9121 ctgctttacg ttttctttac aaacactagc tttgcattga catgttgaat cgcataagca
     9181 ttctccttgt tcttcacagt cacaagcact ttctgttcct gttggagatc cacatccgca
     9241 tgatgcatct gtcatataag ggggatctac tacgtggttt taaaggttat cgaaaaatcg
     9301 caaaattatg aaacggcgtt atagatgcta acttttgatt gttgaaagaa gatcatttgt
     9361 agtttttaat agattagaag cttgtcggtc aattgccaat ggatcatttt cgatatcaag
     9421 tgcaacacga agtatatgca tgaattctaa atttctatgc aaaatgtctt tgactttgat
     9481 gatattattt cgatttgtgt aacagagata aaaatagcaa tcagataaga gtgaatggtg
     9541 tacaagatga tcatattttc tttgtattat ttttgagtga ccgtttgatt ccacaatatc
     9601 agaaaatcct atggtagatt ttatcatcct agaaagaagt gatgtggtgg ctctctctat
     9661 tccaagacat tgatgtacta tagaaaagct ctcattagtt ttattttttt taaatccacg
     9721 aataagttct aacatgtgtg tgggacttgg acgtttagaa ttcaacaaca agactgcatc
     9781 tgcgagaaaa catgcaccgc atttcagcca caagtgcgct agtgcatcag acgttttgac
     9841 tgcatctttt gcttttgtag catagacgcc cgcatcaaca agacagcttt gaatataaga
     9901 attagaaatt ttggcacgtt tttccttaat ctccgacaga aacatgcgga gcttccattg
     9961 atcatctgat attatggtga ggttttgaaa ttggtttaga ataccagagt ttgtttcgaa
    10021 aaactttgca tgatgtagtt ttattatttc tccgcctatt tcgtgcacag attcatgatt
    10081 cttctcgtca aacacagtta tgttatactc acaacattca aaatttgttc catcactcct
    10141 acacccacca agtgcaacag gataatccga gagagaaaat tcttctacaa tattttttag
    10201 attcatagaa aaattacttt tcttttttgc tataaagtat caaagctgtt aaaatttaca
    10261 attcatgcaa caccaaaaca ttgctttaaa ttgctattct ttatggtttt agcaagctcc
    10321 tgtggtgtag tccggctaag cataccgccc tctcaaggcg gtgatcccgg gttcaaatcc
    10381 cggcgggagc atttgaaatt ttttgtctca ggatttactt tatgattatc tagaaatact
    10441 agcaataacg cattaaacat acatggtagg accaaaagat ggtggctttg gttttgatgg
    10501 agctccaaaa tattcagagt tagaaaataa aagcacaata tgtgtacaat gtcaagctgg
    10561 tcaacatgga aaatgtcttg ccaagacaaa tcaacaatta cagtgtgatt gtgagctatg
    10621 tatgctgtcc tagattttat tggatagaca agctagttgg ataccaatta ttctgatatc
    10681 ctagttccaa ggaaccatca aaattctcac caagcgatat tcctcgtata gaaaatgtgt
    10741 cttttgagaa ttttttttct agattatatg gcatatctaa catggcatta taaacagaac
    10801 ccattccgtc tggacatttc acatatatcg attgcgcatt tccatagccc aaacaattct
    10861 cgtaaataat acgagatgga gaattctggt gttgtatgaa gccaagagag gcagagtcca
    10921 tggttttagc attaccaata tcgcttccaa acatttctaa ataacctcct gcagcaaatc
    10981 ctaaaacaaa aaatccggct atactaaaaa tcaacaatgc atcttgtaat ggtgatttgt
    11041 ttacaatcat gtttctgatc atttttacgt gatcgatttg attttgagtc actacattca
    11101 tcttcattcc atttcgtgtt ggaattacac gtaaacagag ctcgtgaaac tgctcttctg
    11161 tttttaagcc acagtccata cacatgcatt gagtgatttt tctacattct tgacatgatg
    11221 aacatgaggt taatggtcct ccacattttc tacatgattc gtctggcata agtcaaaaaa
    11281 tgccaaatta taattaagta atacgaagaa ttcgtactga gaattcatac taaaaactta
    11341 aattctataa gtaaggtggt gccgatcaaa attcaaattt tcttatcaac acaatggatt
    11401 gactttttga ttattttatc attaagaaac tagatgtctt ttgtgattct tctaaccact
    11461 atttgatgac cttcatgtat atgtgaagca tgatttgact ttattatttc agctatcttc
    11521 aattccgaaa aatatctttc attatatctg cccaatgttt ttttacaatt atgacagtat
    11581 atttctctaa aatccacaat aataaaaaga gactaataat attttaagat aaagttagaa
    11641 ttctactggt gacagatcag tatgatcttt attttcaaga aactttattc ttgagatctt
    11701 ataataaatc acttcacctc ttctttctat tactgcaata actggttctt ttcccatttt
    11761 tgttatttgc tctatttgtt tttgtagtaa acccatcttt tccctccttc cctcacttaa
    11821 tccaaatatg acatacttgg cagctttttc accaaactgt cctctttcat atactctgaa
    11881 atcagaaccg aaaccaaaac catcctttgc aatatagccc cttgttcgca aatcacggta
    11941 aatcaagaat tttgtaagaa tatcattatc aaatttgata cattcttgca tcataaaatc
    12001 aaaaggaatt tgttgagttc cccttgtaag tttgagttta tcagtgtaca acagatataa
    12061 tgattcaaat ggttttagaa gaaatttttt ctttacaatt tctccaaatc ctttttgttc
    12121 tagactgtgc tgcatgtttt tatctgaaat caatacatga tcttttaaca acacaccttc
    12181 aactttaaat tcaatatcag acatgctgac tccaactaga aggcaataca atataacatt
    12241 acgttcaata tttcctaggc tagttaagac taagatccat atctacacta tcatcaaaaa
    12301 attgtagcat gaataaaaaa atttcataaa aatctcacgg ttaaaatatg ggttttagat
    12361 aagcagactg agttattatg catggcgcaa attatcgcat cggaaacggt caaccgtaca
    12421 caagaaagaa gtacatcaaa ggcaagcctc aaataaaaat tgccaaattt tctagtggaa
    12481 agagagacga ttatgattat tgtgtccagc tttgttcaag cgagaaggtt cagatcagac
    12541 acatggcaat agaatctgca agattatcag ctaacaaagc aatagaaaca gtagcagggg
    12601 aaacaggtta tttttccatt ctgcgagtat atccacacat cctacttaga gaaaacaaaa
    12661 tgattgcaac tgctggagca gacagacttc aagagggtat gagaggtgct tttggtaaag
    12721 ccgtgagttt ggctgctcgt gttgatagag gtcaatgcat catggaagct tgtgtaaaaa
    12781 aggagcatct tgaagttgca aaaaaggcat tacatggagc tgccgtaaaa ttacccatga
    12841 cacctacaat taaggtaata ccgctaaagg ctgcattagc ctagttttag cgtatcacga
    12901 atttctgtct gtcttttatt tagaatactt agaaattcca taaactcctc ttcagtagga
    12961 ttacgcttta gaattctcct acattgataa taaaatgaat tgtatagtct gccaatcact
    13021 ataccataac caaaggaatc ggaatgagtt cttagctttt caagggaatt tattattttg
    13081 aaaatttcat cagagttttc tgctacctct ttaattttct gattaatttt ttcttctaat
    13141 tttggatcca tggtttgtct agataaaaca agattaaaag tagtgttgct aaccagtacg
    13201 ctttaataga ctaaaatttt ctaaatcgtt taagcggttg tagtacagct tggttaatat
    13261 gcgggattgc caattccgcg acccgggttc aaatcccggc aaccgcatag attatttctt
    13321 taagccattt tttatgactt ctagtgcgtt tacagccctt tcgggttttg gaagttgaat
    13381 catgaagacg ttggatcttc catactcaga atgaggagac gaaagagcta gcatcgaagt
    13441 ggctgcgaga gattcaaaga actcaatgga ctcgttagca taaagcagga tcttttcact
    13501 aattccacca tatgaaaaat taagtgagat ttctacaaat acatgaccca atccatcttg
    13561 aagtatgt
//
LOCUS       DATIDL010000141         2272 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrosopumilaceae archaeon isolate SMAG_U16342
            k141_2393646, whole genome shotgun sequence.
ACCESSION   DATIDL010000141 DATIDL010000000
VERSION     DATIDL010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935836
            Sequence Read Archive: SRR25158326
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosopumilaceae archaeon (soil metagenome)
  ORGANISM  Nitrosopumilaceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrosopumilales;
            Nitrosopumilaceae.
REFERENCE   1  (bases 1 to 2272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/04/2024 05:09:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,520
            CDSs (total)                 :: 1,474
            Genes (coding)               :: 1,474
            CDSs (with protein)          :: 1,474
            Genes (RNA)                  :: 46
            rRNAs                        :: 1 (5S)
            complete rRNAs               :: 1 (5S)
            tRNAs                        :: 43
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2272
                     /organism="Nitrosopumilaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2393646"
                     /isolate="SMAG_U16342"
                     /isolation_source="soil"
                     /db_xref="taxon:2202732"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..672)
                     /locus_tag="VLB45_06325"
     CDS             complement(<1..672)
                     /locus_tag="VLB45_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HSD05347.1"
                     /translation="MVTKNRSLALLLVAAVAVTSVAAIGQAFAQSVTDTEGGLIYTGN
                     ANECWMDSDGDGVMDTACAVDSGHTAWMLTASALVLFMTPGVAFFYGGLARSKNAVNT
                     IGMTFIVIGLISVQWVLWGYSIAFGPIDNDANMFAGSFEYAGLNHVSHLAPLGAPADD
                     GTCLTDASCGSDVWNGLVPHQLWMAFQMTFGIITPALIIGGLIDRMKFSALLVFILLW
                     ASFVYD"
     gene            803..1474
                     /locus_tag="VLB45_06330"
     CDS             803..1474
                     /locus_tag="VLB45_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05348.1"
                     /translation="MKRETAPRLSVFETFKTKREELTGEALRQRSIITHLATESSPAM
                     KTRTAIAHKIAEKNGTVWQNLYSGIFRDLDEILLPLGIVEEAGRLPLKRGPKALQEHG
                     VPHYQLTEQGMLVAASLDELKKTRVEIMIKFLENSNTKDKEFKKVILTLLNVAPSFVF
                     SLLEKYIEAYGNGKVDDLIPFDMNSLKNTRDKIITVHKELLEGFSTLPDPDKKTVIEF
                     LKNVS"
     gene            1517..>2272
                     /locus_tag="VLB45_06335"
     CDS             1517..>2272
                     /locus_tag="VLB45_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HSD05349.1"
                     /translation="MGVGAPQKIFGSVKSFSARGRLLSKGELQTLAESRNLDELVTRI
                     KSTVYVNAVAKLTKPITSEKIESALRSHLADMHYSLAKSMGGGSEVLNAYYLKFLIWN
                     LKLIFKGKVLGKSYEEIEPYVNLHAEELIGRRDVVVKALVAKDVEEAVSTLSTSEFGE
                     ETAKAVALYNEKKNLQVFDTYLDKIFYKHLDSALKSSGEHDLVHLIGMDVDFYNLLSI
                     LRGKFWGLDEQQIQDLIVTHTTSAPKDLLARMIS"
BASE COUNT          769 a          419 c          446 g          638 t
ORIGIN      
        1 atcgtataca aatgatgccc agagaagtat gaaaaccaac aacgcgctga atttcattct
       61 gtcaatgagt ccaccaatga ttagtgctgg tgtgatgata ccgaacgtca tttggaatgc
      121 catccagagt tgatgcggaa ccaaaccgtt ccaaacatcg cttccacatg atgcatctgt
      181 cagacaagtt ccatcatctg caggtgctcc aagcggagct aaatgtgaaa cgtgattgag
      241 acctgcatat tcaaatgaac ctgcaaacat gtttgcatca ttgtctatag gtccaaatgc
      301 tatggaatat ccccacaaaa cccattgaac tgaaatcaat ccaattacta tgaatgtcat
      361 accaattgta ttgactgcgt tctttgatct tgcaagacca ccatagaaga atgctacacc
      421 tggtgtcata aacaggacaa gtgctgatgc tgtaagcatc caagctgtat gtccagaatc
      481 tacggcacaa gctgtatcca ttactccgtc tccgtcacta tccatccaac attcgttggc
      541 gtttcctgtg tagatcagtc caccttcagt atctgtaact gattgagcaa acgcttgccc
      601 aattgctgct acagaggtca cggcaactgc tgcaactagc aataatgcta agctgcgatt
      661 cttcgtaacc attgagaaaa aaaccgccgt aaattatata agcattgaga ttttgtttac
      721 attaaaaggg taaattagca tatttaaata aaaaaccata atatcataat agtttaaatt
      781 tatgttacat taaatatgat gaatgaagag ggaaacagcc ccaagattgt ctgtctttga
      841 gacttttaaa actaaaagag aagagcttac tggagaggct ttacgccaga gatcaatcat
      901 aacacatctt gctacagaat caagtcctgc aatgaaaact aggacggcca tagctcacaa
      961 gattgctgag aaaaatggta ctgtatggca aaacctgtat tctggcatct ttagagattt
     1021 agatgaaatt ctacttccat taggaatagt ggaagaagca ggtagattac cactaaagcg
     1081 tggtccaaag gcattacaag aacatggagt gccacactat caacttactg aacaaggcat
     1141 gcttgttgct gcatcccttg atgaactaaa aaagacacga gtggagatca tgattaaatt
     1201 tcttgaaaat tctaacacca aagacaagga attcaaaaag gttattctga cactgttgaa
     1261 tgtagcgcca agttttgttt tttctttact tgaaaaatac atagaagcat atggaaatgg
     1321 aaaagtggat gatcttattc catttgatat gaacagtttg aaaaatacac gtgacaaaat
     1381 cataacagtc cacaaagaac ttttagaagg attttctact ttaccagatc ctgataagaa
     1441 gacggtaatt gaatttctta aaaatgtcag ctaaccccca aacattaaaa ttcctatcca
     1501 gaggagctgt atcaaaatgg gtgtaggggc tcctcaaaaa atatttggct ctgtcaagtc
     1561 ctttagtgca cgtggaaggt tactttcaaa aggtgagctt cagacattag ctgaatctag
     1621 aaatctagac gaacttgtaa cacgaataaa gagtacagtt tatgttaacg cagttgcaaa
     1681 acttacaaaa ccaatcacgt ctgaaaagat agaatctgca cttagaagtc atcttgcgga
     1741 tatgcattat tcacttgcaa aatcaatggg tggtggttct gaagttctaa atgcatacta
     1801 tctcaagttt ttgatctgga atttgaaatt aatcttcaag ggcaaagtgt taggaaaatc
     1861 gtatgaggaa attgagcctt atgtgaattt gcatgcagaa gaattgatag gtagacgtga
     1921 tgtggttgta aaagctcttg ttgcaaaaga tgtcgaagaa gcagtttcaa ctctttctac
     1981 aagtgagttt ggtgaagaaa ctgcgaaggc tgttgcgttg tacaatgaaa aaaagaatct
     2041 gcaagtgttt gatacgtact tggataaaat tttctataaa cacttggaca gtgcactaaa
     2101 atcttctgga gaacatgatc ttgtacatct cattggaatg gatgttgact tttacaattt
     2161 gcttagcatt ttgcgtggca aattctgggg attggatgaa caacaaatcc aagatctcat
     2221 agtgacacat actacatcag caccaaaaga tttgcttgca agaatgatca gt
//
LOCUS       DATIDL010000142         2681 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrosopumilaceae archaeon isolate SMAG_U16342
            k141_1590922, whole genome shotgun sequence.
ACCESSION   DATIDL010000142 DATIDL010000000
VERSION     DATIDL010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935836
            Sequence Read Archive: SRR25158326
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosopumilaceae archaeon (soil metagenome)
  ORGANISM  Nitrosopumilaceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrosopumilales;
            Nitrosopumilaceae.
REFERENCE   1  (bases 1 to 2681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/04/2024 05:09:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,520
            CDSs (total)                 :: 1,474
            Genes (coding)               :: 1,474
            CDSs (with protein)          :: 1,474
            Genes (RNA)                  :: 46
            rRNAs                        :: 1 (5S)
            complete rRNAs               :: 1 (5S)
            tRNAs                        :: 43
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2681
                     /organism="Nitrosopumilaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1590922"
                     /isolate="SMAG_U16342"
                     /isolation_source="soil"
                     /db_xref="taxon:2202732"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..598
                     /locus_tag="VLB45_06340"
     CDS             <1..598
                     /locus_tag="VLB45_06340"
                     /inference="COORDINATES: protein motif:HMM:NF028479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="6-bladed beta-propeller"
                     /protein_id="HSD05350.1"
                     /translation="NENKVLQYSSIGTFLKSFGATGWKEGQFNKPDSMTIDSDGNLYV
                     TDTGNNRVQKFDSQGKFLVSWGTFGSGPGQFKEPVGIAVDSKKNVYVVDRANNNVQKF
                     APYDAPEDIVIPSWIKNNARWWAEDKIQDSDFAYGIQYLIKQKIINIPEAKLSATDSV
                     KIPSWVKVSAGWWSDDKISDKSFVASIQYLVSVGIIKI"
     gene            complement(595..792)
                     /locus_tag="VLB45_06345"
     CDS             complement(595..792)
                     /locus_tag="VLB45_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007551734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05351.1"
                     /translation="MASKQITIGIGIPMIVVGAILATLLAPTQIGIESTIEFIGSLIG
                     ILGVIIFIAGLFAPKTPQIVT"
     gene            complement(882..1997)
                     /locus_tag="VLB45_06350"
     CDS             complement(882..1997)
                     /locus_tag="VLB45_06350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05352.1"
                     /translation="MKTLTLVLLALVGSMIIIPIQVYADSQLDSLLKIANQARDNIKI
                     RLSQLTTVPGEIAKLYEQGSAETDALAKSVLQADIVASKQHFLSAMKLFKEASDKISS
                     SSPEVVGEPLPATDASRLKNTIIRIEKSAEKLKAVATRNNIQINFTEFDNLIQIAKQN
                     LDANNVDEVDKTLKAANQFILDVHNSISAAAKQKTSDRAKDFATKQINRINAIIKQLE
                     IKLDNLDKESQNDDSRIKTKAARDMIVELKQLVLDGKLEEALQKIKPINEIIASIRIS
                     TTDDTSNTTETENANKVAKVDPTLERVKAKIQTQEEQLNTLSESASDNDIATKWITRA
                     LSLLENAKLQLDKSPEKAMNTLNEIDKIIRMVQRLTA"
     gene            complement(1994..>2681)
                     /locus_tag="VLB45_06355"
     CDS             complement(1994..>2681)
                     /locus_tag="VLB45_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014966110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05353.1"
                     /translation="SLISLPSGPVEINYVFGVLGTSQTATLAIEKAQDLIDKINSEGI
                     ETPQASVKLAEAISAFDQGKYSSAEILANEAKNTATKEQQDALLTPDSNPNTLTALGN
                     TIVGIAATVGTVAGAVIAVAFLIKRSKSAIKKAELLPKNGKYVTPDKETIFRLKPELR
                     QEDKEIVTFISENGGQVYESELRKKFLLPRTTTWRAVKRLEREGIVEIEKIDQQNLIK
                     LRQIAGENQE"
BASE COUNT          740 a          449 c          524 g          968 t
ORIGIN      
        1 taacgaaaat aaggttttgc agtatagtag tataggtaca tttctcaagt catttggagc
       61 gacagggtgg aaagaaggcc agttcaacaa gccagactct atgacaatag attctgatgg
      121 aaatctctac gttacagata caggtaacaa cagagtacag aaatttgatt cacaaggaaa
      181 atttctagtt agttggggta ctttcggaag tggaccagga cagttcaagg aaccggttgg
      241 aatagcagta gattccaaaa aaaatgtgta tgtagtagat agagcaaata acaatgttca
      301 aaaatttgca ccttatgatg cgcctgagga cattgtgata ccaagctgga tcaaaaacaa
      361 tgcacgatgg tgggcagaag acaaaatcca agattctgac tttgcatatg gcatacaata
      421 tttgataaaa caaaaaataa tcaacattcc tgaagccaag ctgtcagcaa cagattctgt
      481 taaaattcca tcatgggtca aagttagtgc agggtggtgg tctgatgaca aaatatcaga
      541 taaaagtttt gtagcaagta ttcaatacct tgtttctgtt ggaataataa agatctaagt
      601 tacaatttga ggagtttttg gggcaaacaa tcctgcaatg aagattatga caccaagtat
      661 tccaataaga ctaccaataa attctattgt actttcaatg ccgatttgag taggggcaag
      721 taatgttgcc aatattgcgc caactacaat cataggtatt ccaataccaa ttgtgatttg
      781 tttagaagcc atgtgggtct agccatgaaa aacgtatatg aagtttatgt ataggttttt
      841 gttatactag aagtaatttt tataaaaatt taggcggttg cttaggctgt tagtctttgt
      901 accatgcgaa taattttatc aatttcgttt aatgtgttca tagctttttc tggagatttg
      961 tcaagctgaa gctttgcgtt ttcaagaagt gataatgcac gagtaatcca tttggtagca
     1021 atgtcattat cacttgcact ttcagataga gtgtttagtt gttcctcttg agtttgaatt
     1081 tttgctttta ctctttctag tgttggatca acttttgcaa ctttatttgc attctcagtt
     1141 tctgtagtgt tacttgtatc atccgtagta gaaattctta tgcttgcgat aatttcgttt
     1201 attggtttta ttttttgcag tgcctcttca agcttgccat ccaagacaag ttgttttagt
     1261 tccacaatca tatctcttgc agcttttgtt ttgattctag aatcatcatt ttgtgattct
     1321 ttatccaaat tgtctagttt tatctcaagt tgttttatga tagcattgat tctgttgatc
     1381 tgttttgtgg caaagtcttt ggctctgtct gatgtttttt gtttagctgc ggctgaaatt
     1441 gagttatgta catccagtat aaactgattt gctgctttga gtgttttatc tacctcatct
     1501 acattgttag catctaagtt ttgttttgca atttgtatca gattatcaaa ttccgtgaaa
     1561 ttaatctgga tgttgttcct agtcgcaact gccttaagtt tctctgccga tttttctatt
     1621 ctgatgattg tattttttaa tctggatgcg tctgttgcag gtagtggttc cccaactact
     1681 tcaggactag aggatgatat cttatcgctt gcctccttga ataatttcat tgcagaaaga
     1741 aagtgttgtt ttgaagcaac aatatctgcc tgtaaaactg attttgcaag tgcatctgtc
     1801 tctgcggaac cttgttcata gagtttagca atttcaccag gtacagttgt aagttgtgat
     1861 aatctgatct ttatgttatc tctagcttgg tttgctattt tgagtaacga atcaagctgt
     1921 gaatcagcat aaacttggat tggaataata atcatacttc caactagtgc aagtagaaca
     1981 agtgttaatg ttttcattct tgattctccc ccgctatttg tcgcaatttt atcagatttt
     2041 gctgatcaat cttctctatt tctacaattc cttccctttc aagacgtttt actgcacgcc
     2101 atgtagtcgt acgtggaaga aggaactttt tgcgtaattc actttcatat acttgacccc
     2161 cattttcaga gatgaatgta actatttcct tgtcctcttg tcgtagttca ggtttaagtc
     2221 tgaatatggt ttctttatca ggtgttacgt atttgccgtt cttaggtagg agttctgctt
     2281 ttttgattgc agatttggaa cgtttaatca aaaatgcgac tgcgattaca gctcctgcta
     2341 ctgtacctac agttgctgct attcctacaa tggtgttacc tagcgcagtt agggtattgg
     2401 gattagagtc tggtgttaag agagcatcct gttgttcttt agtagcagtg ttctttgctt
     2461 catttgctag aatttctgca ctagaatatt tgccctgatc aaatgcggaa attgcttctg
     2521 caagctttac tgaagcttgc ggtgtttcaa ttccttcaga attaatttta tcaattagat
     2581 cttgtgcttt ttcaatagca agtgtagcag tctgagatgt tcctaagaca ccaaacacat
     2641 agttaatttc aacaggacca cttggcaatg aaataagtga t
//
LOCUS       DATIDL010000143        18916 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrosopumilaceae archaeon isolate SMAG_U16342
            k141_2599281, whole genome shotgun sequence.
ACCESSION   DATIDL010000143 DATIDL010000000
VERSION     DATIDL010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935836
            Sequence Read Archive: SRR25158326
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosopumilaceae archaeon (soil metagenome)
  ORGANISM  Nitrosopumilaceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrosopumilales;
            Nitrosopumilaceae.
REFERENCE   1  (bases 1 to 18916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18916)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/04/2024 05:09:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,520
            CDSs (total)                 :: 1,474
            Genes (coding)               :: 1,474
            CDSs (with protein)          :: 1,474
            Genes (RNA)                  :: 46
            rRNAs                        :: 1 (5S)
            complete rRNAs               :: 1 (5S)
            tRNAs                        :: 43
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18916
                     /organism="Nitrosopumilaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2599281"
                     /isolate="SMAG_U16342"
                     /isolation_source="soil"
                     /db_xref="taxon:2202732"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1094
                     /gene="ftsY"
                     /locus_tag="VLB45_06360"
     CDS             <1..1094
                     /gene="ftsY"
                     /locus_tag="VLB45_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HSD05354.1"
                     /translation="ALRENVSNMFDAAGQIDILQNIKKKNETGDPYLILFLGINGTGK
                     TTTLAKFANLLRENKFSVAIAAADTYRAGAIEQLSEHAKRLNLKIIAQNYGSDPAAVA
                     RDAVLYAKSHKIDCILIDTAGRMQTSKNLMDQISKINKVVNPDLKIFVGDSLAGNDTV
                     NQAREFHQYTNFDAAILTKSDADARGGAAISIVKVTSAPILYLGVGQEYKDLKVFDKE
                     SFLETIFGEATQESMPEIQIPQTKNSKDEKPSELKPEQQVPTPEIKKEIRIESQPTQK
                     PESKPEVVEIDNDDPFANIATKDIEKYSKMYDVPPPETDKEAVELAAKIRKWISQGRP
                     KPEEEQSGSVDQEDAKDKKKHSLFGWGRK"
     gene            1091..2005
                     /gene="argF"
                     /locus_tag="VLB45_06365"
     CDS             1091..2005
                     /gene="argF"
                     /locus_tag="VLB45_06365"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HSD05355.1"
                     /translation="MKLKTKNLLTLNELDKKEIEGIIDNAIKLKKELKAGIYKPLLKN
                     KTLAMIFQKPSTRTRVSFETGMFQLGGHALSLSSDDLQLARGETIEDTSKTLSRYVDI
                     IMARVYSHEEVEMLAKSATVPVINGLSDSFHPCQILADLMTIKEKKKKLAGLKLAWIG
                     DGNNVCNSMIYGCAKTGIDISIATPKGYEPDSKIIKESKNLVDLELLHDPQVAVKDAD
                     VVVTDTFVSIHHQATDRTKDFLPKFQVNSSLMKKAKSNAIFLHCLPAKRGQEVTSSVI
                     DGSQSAVWDEAENRLHAQKALMIALLRV"
     gene            2054..2542
                     /locus_tag="VLB45_06370"
     CDS             2054..2542
                     /locus_tag="VLB45_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HSD05356.1"
                     /translation="MGYSQILRRLREEKTNYRKRKHLLMGRRDFITVQISSENTLVQV
                     HKPEMAGDKVLASAHSRSLLNKGWKGSRKSIPAAYLTGYMAGKKALTKGTKDAVLYSG
                     TRRYTQRMAAALKGVIDAGLQVPADKETFPDEERLTGKHLKVANDINKIKSAIDGEVK
                     TK"
     gene            2539..3174
                     /locus_tag="VLB45_06375"
     CDS             2539..3174
                     /locus_tag="VLB45_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007549792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HSD05357.1"
                     /translation="MSQTEQRVRRERKEQEELWIPRTKLGTMVNSGKITTMKEIYENG
                     YRIQEAGIIKKLLPGIKTEVIDVGIVQKQTTNGEYTRFKALVAAGDESGWLGIGQGKS
                     KQMRIAIEKATNAAYLAVSPVKLGCGSWECRCDKPHSVPFKVRGRGGSVTVEIIPGPR
                     GLGLVAGGNIRNLLKLAGLKDVWTKSKGSTNTMTSTSRAVLNCLRQTFSQG"
     gene            3181..3648
                     /locus_tag="VLB45_06380"
     CDS             3181..3648
                     /locus_tag="VLB45_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HSD05358.1"
                     /translation="MANSFLVVRMRGTVNVPHWANTTLDLLHLDKKFRATIIPSKDNT
                     KGMLDKIKHYVSWQELDIPTTKELLEKKARKGDYKKITTEDITKLGFKNTDELAKSLV
                     DGTATLSKLKPLKPWFALSPPRHGFKRNTKKMYGEGGILGSHKELLAQVRHMI"
     gene            3650..4072
                     /locus_tag="VLB45_06385"
     CDS             3650..4072
                     /locus_tag="VLB45_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uL15 family ribosomal protein"
                     /protein_id="HSD05359.1"
                     /translation="MATRLRKTRKFRGGRNHGWGQIGQHRASGHKGGLGRSGMHKHHF
                     IKMLLTDPNHFGHDSTHPPHPNLVRKWISVRDLDAFFAKFGKQEGGKKVIDLTELGYD
                     KLLGGGQTKNAYTIKVERFSATAEEKIKQAGGEVLAEA"
     gene            4069..5505
                     /gene="secY"
                     /locus_tag="VLB45_06390"
     CDS             4069..5505
                     /gene="secY"
                     /locus_tag="VLB45_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HSD05360.1"
                     /translation="MSEEGTPTGILRKIVQKAEVYIPQVPKPKKKLSLQVRLMWCGLA
                     LFIYMIMSQTPLYGATTPEFDFLAFARVIFASQQGTLVELGIGPIVTAGLLMQLLKGS
                     EIFHLDFKKPEDREMFQTATKMVTFIVIVAESALYGMSVYGPRLPSPEMGYILIGQLI
                     GASIIIMFLDELIQKGWGLGSGISLFIAAGVAQQIMWSMFSPISAGDGGLTGVIVNIG
                     NSLANGGNLFDLFFRANQLPSIFGLFLTGGILLILVYTQGIKIEIPIVSTKYRGFSAV
                     YPIKLMYVSNIPVILASALTANAVFVGQLLWANYNPRNNNVFMNFIGQFDPTSPSTPI
                     GGILYYITPPRGLDIAVLDPLRAVLYVLFMIGIVVLFGRLWVELGGLSAKKAAQNLLD
                     ADVQVPGFRRSNQPVENILQKYIPSVTILGSMILGGLAGVSDVLGVFGSGIGILLTVD
                     ILINYYNLLVREQVETVMPRLGALLGRK"
     gene            5483..6082
                     /locus_tag="VLB45_06395"
     CDS             5483..6082
                     /locus_tag="VLB45_06395"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HSD05361.1"
                     /translation="MHCLAESKTVIIVGIPGVGKTTLVSKVVEILQAKKISTSVSIFG
                     TVMFDEAKKNGIKSRDDLRKLSVPEQKKLQSMAAKKIASLSDDVIIVDTHAFIATKEG
                     FYPGLPHNVLDILKPDSFIMITARPEEIYNRRMKDTTRDRDIVSIDAIKKELDVTSAM
                     LSTCSILCGSPIKMVLNTEGKIDEVAKAIVSAMGYNNGS"
     gene            6072..6689
                     /locus_tag="VLB45_06400"
     CDS             6072..6689
                     /locus_tag="VLB45_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016939579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EMC3/TMCO1 family protein"
                     /protein_id="HSD05362.1"
                     /translation="MEANFIFAFLDSILLQIPGLTSGPLGSADPIIKGMIPSAFGIVG
                     IAICLNLFNALIRRKMVDQGKLKRLMKETRSWQKERMAAFKAKDQDKINELNKKSAYM
                     NKMNMELMQMNMRPMMITFIPLMLIFYFVLPPLFAYTVAISPMSLNFVPVDFFQLTCT
                     AEKVAATPNICKHENEVYFWAWYFLASIAFSGIIMRVTKTTMDQN"
     gene            6691..7251
                     /locus_tag="VLB45_06405"
     CDS             6691..7251
                     /locus_tag="VLB45_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HSD05363.1"
                     /translation="MPKSVIISGPPAIGKTTVAKGLAKEFNMKYLSGGDVLKDLAQEE
                     GFQTEGDDWWDRPDGMKFLNQRKTNPEFDKKVDNKLKELFSKGGVVITSYTLPWLVKD
                     GIKIWLAGTQENSAKRMTTRDNLSAKDALAIVKKRYDENKMIYKKLYGFDFGENLSVF
                     DKIIDTDDLGADEVLLVAKSVVRKLL"
     gene            7248..8246
                     /locus_tag="VLB45_06410"
     CDS             7248..8246
                     /locus_tag="VLB45_06410"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007549801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided pseudouridylation complex
                     pseudouridine synthase subunit Cbf5"
                     /protein_id="HSD05364.1"
                     /translation="MKLKQLQNLKVIDQDITNEEHGTYYDKRSVDQLLNYGLILLDKP
                     NGPTSHETVAWVKKIFNIQKAGHSGTLDPPVSGLLPIGFGEATKALSVLLLGPKEYHA
                     LGRLHTLPSSEKLDSVLSQFKGKIHQKPPQRSSVSRQTRIRNIYEIEVIEQKERLLLL
                     RILCEAGTYIRKIFYDMGEILGPGATMIELRRTRVGHFSEDSNLVKMHDLVDAYALWK
                     EKGDDSKILRIIMPIEYALSEIKSVVIRDSAVDALCHGAQLAIPGILEISPNIKKGDY
                     VGIYTQKGEIVALGEAILSESEIIENTKGFAFQTRRIIMAPNTYPKNWRSKTIVNK"
     gene            8273..8722
                     /locus_tag="VLB45_06415"
     CDS             8273..8722
                     /locus_tag="VLB45_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05365.1"
                     /translation="MVPKGAIIGLCAGITGAVVLGLFLANIANQVPVIVYVDGPSLSI
                     MTEKTNFELGEAVTIKIINSGTVPLTFSDSSYGLKIVGLDGRILYSPPSIQTISILQP
                     NEEKTFVWDQTKSDGDKIIEGRYKIVSSTISSGENVLKKSLSINILK"
     gene            complement(8727..10520)
                     /locus_tag="VLB45_06420"
     CDS             complement(8727..10520)
                     /locus_tag="VLB45_06420"
                     /inference="COORDINATES: protein motif:HMM:NF024299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSD05366.1"
                     /translation="MIKKSIFFVIFTILIFSLNSNFALAEEEQDEPIGDIIISTDKPA
                     YVPEETIVISGEITKRKMPLIAIKVFDPEDNILGGYNVELDENNYFSKTVKADIPFYE
                     KPGIYTVIAEYGKIKSEIIFEIENDLFVEEEILEAEDILESDEILVEEVKPQITLFKT
                     DKETYQDGDLISITGKVSKNSLPQVTITIFDQFDIPTGIYLATVNPDLTFSTSFLAKY
                     NTNFKVIGQYTVTAQYGELETKQILTIEFVEKGSLSESTGSAPEIEEPTTDSFLFSES
                     DLQHLASWYYLGSTDDDLVLFFEDLAKKDLLNSDTNTVISKNLLSKWIRANNLPIGSM
                     ISDLFEGNISEERFILLIENSLNDYIDTSSNTVSPPQQKPISEIKKPENTNPKKSEDT
                     PTEAKNELPLEPEEEKITYFYSNVNCEKKTYEDVISQYNSPGPALAHLCKYEDAILHY
                     ERTLEHEPENIHALTNTGSALANLGRYDDALLYYDKVLEIDPNNLAALHNKGNVLADL
                     GNFQEAISYYDQVLKTNPHYLPTLENREKASTSLTVFTTNEEPHMQSISHHVTEDNEQ
                     DSISETNSDIVKQFASALSSIGTSLLALFGG"
     gene            10683..10779
                     /locus_tag="VLB45_06425"
     tRNA            join(10683..10720,10734..10779)
                     /locus_tag="VLB45_06425"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10717..10719,aa:Ser,seq:gga)
     gene            10825..12138
                     /locus_tag="VLB45_06430"
     CDS             10825..12138
                     /locus_tag="VLB45_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HSD05367.1"
                     /translation="MAKYDVAIIGGGILGTSISYWISSLYNAKICVIEKEPEVAMHAS
                     SRNTGVLHSPFYLDPVKKKILAKSAFISHALWKTLAAQKKLPWKNVGTLEVALDENQH
                     KTLEKYMKWGTENGISQNDIELLDSNDVSKREPNVRCHSAVFCKTDVGVDYGAFTREI
                     KDESQRNGTQFLLKHNVKSIDSKEKESTIKFQDNTELTSKLIINCAGGNSLDIAKMVG
                     IAEEYSDLHFRGEYWIADDQYADLVKTNVYSVAKLPNFPFLDPHWIRRADGTTEIGPN
                     AVPVMSAETYSGYLGEASTSLSKIAEIVTGNARKLLLNPDFISLISKEWLSSISKTVM
                     IERVQQFIPKIRSNFFNKRGTAGIRTPLITPEGTFLPDVLEIEGVNSFHVLNYNSPGA
                     TGAPAYSAYVVKRLQEKGLLNNATQKKSSIWNFEEIIAKLNILSA"
     gene            12151..12411
                     /locus_tag="VLB45_06435"
     CDS             12151..12411
                     /locus_tag="VLB45_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05368.1"
                     /translation="MLPEHCSIKEKNHDCPMPPEFILSIKTKEDEYMVGVACGGHKQT
                     FFEKLEQLQKDGKIPDGTIKFTGLKAVGTDCIRMDPDDLIQL"
     gene            complement(12408..13379)
                     /locus_tag="VLB45_06440"
     CDS             complement(12408..13379)
                     /locus_tag="VLB45_06440"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HSD05369.1"
                     /translation="MKLKVLLTRTLPDFAISELRKHYDIEINHGKIPMPKSKLISKIR
                     NKDGLICFPYDTIDAEVIDAAKNLRAISTFSVGYDHIDIRHAAKKGIVLGYTPEVLTR
                     ATADHTIALILSLLRKVVEGDRIIRQNKWKVIFGSYDFLGSDLSGKIIGIFGMGRIGK
                     AVAKRAKGFEMDILYYNRHRLTKNEEKNLGIRYVSLKRLFEESDIVSIHTPYTKETHQ
                     IINLQFLKRMKKTAVLINTARGKIIKEKDLIFALQKKIIAGAALDVFQKEPIGKNHPL
                     VRMKNVVLTPHIGSSTEETRRKMVEITVRNMILSLTGKNPIYIVKAS"
     gene            13489..14430
                     /locus_tag="VLB45_06445"
     CDS             13489..14430
                     /locus_tag="VLB45_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HSD05370.1"
                     /translation="MGRGFKQILQESPCYFHIGMKYAAYKLFNKRSPFYGSADIINVC
                     NLHCTHCYWWLNRKENEELTTEQWKEIIEQKFKKQHVFIITLVGGEPTLRPDVIELFV
                     KEFPKRVCIVSNGTYPLKKYDDLYFYWISVDGTQEIHDSIRGNGAYASTRKNILDYVE
                     KNGDKAWKDIWISMTINSLNYKTVKKVAEEWYEYTNKIGFQFHTPFTKGDPLWLPFGP
                     ERDQVVDDIIAMIDKYDDHYVINPKSQLELMKKNWGGRGTTPLDCPTWAILSVDHMGR
                     EKLPCCIGSADKESMKPICEDCGLGCYSVLVGYGIRG"
     gene            14490..14621
                     /locus_tag="VLB45_06450"
     CDS             14490..14621
                     /locus_tag="VLB45_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05371.1"
                     /translation="MDQEEFALCIQCGNSLEKCICVCPFCGERDKCECALFDAATGG"
     gene            14664..16577
                     /locus_tag="VLB45_06455"
     CDS             14664..16577
                     /locus_tag="VLB45_06455"
                     /EC_number="1.2.7.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018491 - 2-oxobutyrate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0019164 - pyruvate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0047553 - 2-oxoglutarate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HSD05372.1"
                     /translation="MNSVDITWVIGGPQGSGVESAANIFARACAATGLQIFGKREFYS
                     NIKGEHSYFAVRVSDKKIHSNVSGITLLVAFDAETLFRHADDVYHDGAIIYDSDLAQT
                     KVDEVHTLDEPFKSRLAKLLSSKNKTASIQGLLDICKDNGVQLHPISFKDILSNLADE
                     IQNPAVKNMFRMLNVLCVSVSLGFLDLPPTTLLESVGSIFSAKPKIAELNKHAANYAY
                     NFATVKFPKSKYVLPSVQKESNTLLIQGHQAAGLGKIACGCRFQSYYPITPASDESVF
                     LESNEVFDVQDNRPGSTLVVQTEDEISAIGMVIGSSLTGTRSATSTSGPGFSLMAEAL
                     GWAGINEVPIVVTLYQRSGPSTGLPTRHGQDDLMFAIHAGHGDFPKIVYASGEVEEIF
                     YDTAKCFNFSELFQVPVIHILDKFLASTVKTCKRFDPNLMDINRGKLLEKIDSDDYKR
                     FAFTEDGVSPRSKLGLENGIFWNTGDESDEQGHISEDPEIRVKMMDKRASKLELALKT
                     IPLEDQVVSFGVSEFCVVSWGSTKGPILDAMEMLKKEGYRLGFVQIKLLQPFPKDYLK
                     SLLKNSKVIIDIEANHSAQLGSLLNENLDKKVDYYILKYTGRSMTSTEIYHSLKKILE
                     KKADKRQVLRYGA"
     gene            16567..17532
                     /locus_tag="VLB45_06460"
     CDS             16567..17532
                     /locus_tag="VLB45_06460"
                     /EC_number="1.2.7.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008301344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018491 - 2-oxobutyrate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0019164 - pyruvate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0047553 - 2-oxoglutarate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HSD05373.1"
                     /translation="MEPKLADFKTEVHNDWCAGCGDFGIVNAIQMALTEMKIPRHKTA
                     LFSGIGCSGKTSHFVNVYGVHTLHGRVLTFAQGAKLANPDMTVVAAGGDGDGLGIGAG
                     HFVAAGRRNVDMTYIIFNNAVYGLTKGQASPTLQLGKQTKSLPNPNVNQAVNPIGLAI
                     ASGFTFVGRGYAYDVRQLKDLIVSAVKHKGLAFLDVLQPCPTYNDINTRDWFAGLDNI
                     DEVTKKSRPRIYKLEDTGYDPVVHYGSEQEVNEKLTQALVRSNEWDAKIPTGIFYQNE
                     LVAPYGERMADRIPNYLENPPASQPIASNGKPITDISKILDSLEV"
     gene            17570..18490
                     /locus_tag="VLB45_06465"
     CDS             17570..18490
                     /locus_tag="VLB45_06465"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HSD05374.1"
                     /translation="MKIFVTGGAGFIGRHLIASLLKSNKVTIYDNFKNSTKGKISHLV
                     KNGAIIIQGDVTDYKTLSKSVKGSDLVIHLAAEIDVQKSIKSPELIHHVNVTGTVNLL
                     RACVAHKVSNVIAASSAAVFGNLKDLPLSENSPTVPISPYGASKLAIESYMQAFSHAY
                     DLNCITLRFFNIYGKGQSNAYAGVITKFMESIAHNKPLVIFGNGSNTRDFVSVDDVVQ
                     SIQKAIAKIKGKKGNFYNIGSGKFTTINDLAKLMISLSGKNLSIKHKKPKKGDIQHSQ
                     TSIWLAQRELGYVPKVMLKDGLERLLESTS"
     gene            18531..>18916
                     /locus_tag="VLB45_06470"
     CDS             18531..>18916
                     /locus_tag="VLB45_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007549815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05375.1"
                     /translation="MNLSEVNKVFRKSIIKGYFEPRLLSLDFKKSNVKHPSIQDDGLM
                     QSNLLYLFFDIDTGSDYPDGDEWFMAEFLFPYSIKIPDGIKGPDYFSTISVGEGKNFW
                     RHRELIRYKYGKSKKLEESLDFIEKK"
BASE COUNT         6464 a         3262 c         3739 g         5451 t
ORIGIN      
        1 aagccctgcg agaaaacgtt tctaacatgt ttgatgcagc tgggcaaatt gacatattac
       61 aaaacataaa aaagaaaaat gaaaccgggg acccttatct gattttgttt ttgggaataa
      121 acggaactgg aaagactaca acactggcta aatttgcaaa tcttttacgt gaaaataaat
      181 tttctgtagc tattgcagca gctgatacct atagggcagg ggcaattgaa cagcttagcg
      241 aacacgcaaa gaggcttaat ctaaaaatca tagcacaaaa ctatggctct gatcctgctg
      301 ccgtagcacg tgacgcagta ttgtatgcca agtcgcacaa aatagattgt atcctaattg
      361 atactgcagg caggatgcag acaagcaaga acctcatgga ccagatctca aaaatcaaca
      421 aagtcgtaaa tcctgatttg aaaatttttg ttggagattc acttgctggc aatgatacgg
      481 tgaaccaagc acgagaattt catcaataca caaactttga tgctgccatt cttaccaaaa
      541 gtgatgccga tgcacgaggc ggtgcagcta tttctatagt aaaggtcaca tctgcaccaa
      601 ttctgtatct tggagtaggt caggaataca aggacttgaa ggtgtttgac aaagaatcat
      661 ttttagaaac aatctttgga gaagcaacac aggaatcaat gccagaaatt caaattccac
      721 agacaaagaa ctctaaagat gaaaagccat ctgaattgaa accagaacaa caagtaccaa
      781 caccagaaat taagaaagag attagaatag aatcacaacc tacacaaaaa ccagaatcaa
      841 aacctgaggt tgttgaaata gacaatgatg atccatttgc aaacattgca acaaaggaca
      901 ttgaaaaata ttcaaagatg tatgatgtac caccaccgga aactgataaa gaggcagtag
      961 aattagctgc aaaaatcaga aagtggatct cacaaggcag accaaaacct gaagaggaac
     1021 aatctggcag tgtagatcaa gaagatgcta aagataaaaa gaagcattct ctatttggtt
     1081 ggggaagaaa atgaaactca aaacaaaaaa tcttctaaca ttaaacgagc ttgataaaaa
     1141 ggaaattgaa ggcatcattg ataatgccat aaaattgaaa aaagaactca aagctggtat
     1201 atacaagcct ctcctgaaga acaagaccct tgcaatgata tttcaaaaac catcaacacg
     1261 tacacgtgta agctttgaaa caggaatgtt tcagcttggt ggtcatgcat taagtctatc
     1321 ttcagatgac ttgcaacttg cgcgcggaga aacaatcgaa gatacatcaa agacactctc
     1381 taggtacgtg gatatcataa tggcacgtgt atattcgcac gaggaagtag aaatgctagc
     1441 aaaaagtgcc acagttcctg tgataaatgg actgtcagat tcttttcatc cttgccaaat
     1501 actggcagac cttatgacaa taaaagaaaa aaagaaaaaa cttgctggtc taaaacttgc
     1561 atggattgga gacggaaata acgtctgcaa ctctatgatt tatggatgtg caaaaacagg
     1621 aattgatatt tctattgcaa ctccaaaagg atatgaacca gacagcaaga tcataaaaga
     1681 atcaaaaaat ttggttgatt tagaattgtt gcatgatcca caagtagctg taaaagatgc
     1741 tgacgttgta gtaactgata cattcgtgtc tattcatcat caagcaactg ataggacaaa
     1801 agactttttg ccaaagtttc aggtgaattc atctttgatg aaaaaagcaa aatctaatgc
     1861 aatctttctg cattgtctcc cagcaaaaag aggacaggaa gtgacaagct cagtaataga
     1921 tggatctcaa tcagctgtgt gggatgaagc tgaaaaccga ttacatgctc aaaaagctct
     1981 gatgattgca ctgctccgag tctaacgttt ataagagctt taccgaaatt ttttgtaaaa
     2041 gaggtatagt atattgggat attcgcaaat attacgcaga ttaagggaag agaagacaaa
     2101 ctacagaaaa aggaaacatc ttctcatggg taggagagac tttatcacag tacaaatttc
     2161 tagtgaaaac actctggtcc aagtccataa accagaaatg gctggagaca aagtactagc
     2221 atctgcacat tctagatctc ttttgaacaa gggctggaag ggttcacgaa aaagcatacc
     2281 agctgcatat cttactgggt atatggcagg aaaaaaagca cttacaaaag gaacaaaaga
     2341 tgctgttctt tatagtggaa caagaagata tacgcaaaga atggctgcag cactaaaagg
     2401 tgtaattgat gcaggtctac aagttccagc agacaaagaa actttcccag atgaagaaag
     2461 attaactggt aaacatctca aagttgcaaa tgatattaac aaaataaaat ctgctataga
     2521 tggagaggtc aaaactaaat gagtcaaaca gaacaaagag ttcgacgtga aagaaaagaa
     2581 caagaagaac tgtggatccc aagaacaaag cttggcacaa tggtaaattc tggaaagatt
     2641 acaacaatga aagaaattta cgaaaacggt tacagaatac aagaagcagg aataattaag
     2701 aaacttttac caggaattaa aactgaagta attgatgtcg gcattgtaca aaagcaaact
     2761 actaacggtg aatatactcg attcaaagca cttgttgcag ctggtgatga aagtggatgg
     2821 cttggaatag gtcaaggaaa atcaaaacaa atgagaattg cgattgaaaa ggctacaaac
     2881 gctgcatatc ttgctgtcag tccagtaaaa ctaggttgtg gaagctggga atgcagatgc
     2941 gataaaccac actctgttcc attcaaagta agaggcagag gagggagcgt aacagttgaa
     3001 attattccag gaccaagagg tctcggactg gttgctggag gaaacattag aaaccttctg
     3061 aaacttgcag gactaaaaga tgtgtggaca aagagtaaag gttctacaaa tacaatgact
     3121 tctacatcta gggctgttct aaactgtctt agacagacat ttagtcaagg ttgattgata
     3181 atggcaaatt ccttcttggt tgtgcgaatg cgaggaacag taaacgttcc gcattgggca
     3241 aataccacgc ttgatctttt acatttagac aaaaaattca gagctacgat aattccatca
     3301 aaagacaaca caaaaggaat gctagataag ataaagcact atgtgtcttg gcaggaactt
     3361 gacataccaa ctaccaaaga gcttttagaa aaaaaggcac gtaaaggtga ttataaaaaa
     3421 attacaactg aagatattac taaacttgga ttcaaaaaca ctgacgagct tgcaaaatct
     3481 ctagtagatg gaactgctac attatcaaaa ttaaagccac taaaaccttg gtttgcattg
     3541 tcacctccaa gacacggttt caagcgaaac acaaagaaga tgtatggaga aggtggaatc
     3601 ttgggtagtc acaaagaact gcttgcacaa gtgaggcaca tgatataaaa tggcaacaag
     3661 attgagaaag acaagaaaat ttagaggtgg acgaaaccat ggatggggac aaattggtca
     3721 acatagagca agtggtcaca aaggtggtct tggcagatcc ggcatgcaca aacaccattt
     3781 cattaagatg cttctaacag atccaaatca ttttggtcat gactctactc acccaccgca
     3841 cccaaacctt gtgagaaaat ggattagtgt aagagatctc gatgccttct ttgctaaatt
     3901 tggaaagcaa gaaggtggaa aaaaggtaat agatcttaca gaacttggtt atgataagct
     3961 tctaggtgga ggccagacca agaatgctta cactatcaaa gttgaaagat tctctgccac
     4021 tgcagaagaa aaaataaaac aagctggggg cgaggtgcta gcagaggcat gagtgaagaa
     4081 ggaacaccaa ctggtatttt aagaaaaata gtacaaaaag ccgaagtcta catcccacag
     4141 gtaccaaaac ccaaaaagaa actttctttg caggtgcgtc tgatgtggtg tggtttagct
     4201 cttttcatat acatgataat gtcccaaact cctctgtatg gggcaacgac tccagaattt
     4261 gattttcttg catttgcaag agtaatcttt gcatctcaac aaggaacgct ggttgaactt
     4321 ggaattggac ctatagtgac tgctggactt ttaatgcagc ttctgaaagg ttctgaaatt
     4381 tttcatcttg attttaagaa accagaagac agagagatgt ttcaaactgc aactaaaatg
     4441 gtcacattta ttgtaattgt tgccgagtct gcactatatg gaatgtctgt ttatggacca
     4501 agactgccat cgcctgaaat gggttacatt ctgataggtc agcttatcgg ggcttctata
     4561 atcataatgt tcttagatga actgattcag aaaggatggg gattgggaag tggaataagt
     4621 ctctttattg cagcaggtgt agcccaacaa ataatgtgga gcatgtttag tccaatttct
     4681 gcaggagatg gtggtctaac aggtgtaatc gtaaatattg gaaattcgct tgcaaatgga
     4741 ggtaatttgt ttgatttatt tttcagagct aatcaattac caagtatctt tggattattt
     4801 ctgacaggag gcattctgtt gatccttgtt tatactcaag gaataaaaat tgaaattcct
     4861 attgtctcta caaagtatag aggattctct gcagtttatc caatcaaatt gatgtatgtg
     4921 tcaaacatcc cagtaatttt agcatctgca cttactgcaa acgcagtgtt tgttgggcag
     4981 ctactctggg caaactataa tcctcgtaat aacaatgtct tcatgaattt cataggtcag
     5041 tttgatccga ctagcccttc tactccaata ggtggcatat tatactacat tacaccgcca
     5101 agaggactgg atattgcagt attagatcct ctaagagcag tactctatgt cttattcatg
     5161 ataggaatcg tagtattgtt tggaagactc tgggtagagc ttggtggtct atctgctaaa
     5221 aaagctgcac aaaaccttct tgatgcagat gtacaagtac caggattcag acgttcgaat
     5281 caacccgtag aaaacatact gcagaaatac attccatctg ttacaatact tggttctatg
     5341 attcttggcg gattagctgg tgtatctgac gttttgggtg tatttggttc tggaattgga
     5401 atattgctta cggtagacat tctgattaat tattataact tgctagttcg agaacaagta
     5461 gaaacagtaa tgccaaggct tggtgcattg cttggcagaa agtaagactg taataattgt
     5521 tggaattcct ggagtaggaa agacaacgct agtatccaaa gttgtagaaa tattacaagc
     5581 aaaaaagatc agtaccagtg tttctatttt tggtactgtc atgtttgatg aagcaaagaa
     5641 aaatggaata aaaagtagag atgacttgcg taaactctct gtgcctgaac aaaaaaagct
     5701 tcaatccatg gcagccaaaa aaattgcttc actttctgat gatgttataa tagtagacac
     5761 ccacgcattc attgctacaa aagaaggatt ttaccctggg ctaccacata atgtcttgga
     5821 cattttaaaa cctgatagct ttatcatgat cactgcaaga cctgaggaaa tctataaccg
     5881 aagaatgaaa gatactacgc gagaccgtga tattgtttca attgatgcaa ttaaaaagga
     5941 acttgatgtg acaagtgcta tgctttctac atgttcaatt ttatgcggtt caccaataaa
     6001 gatggtatta aacactgaag gaaagattga tgaagttgct aaagcaattg taagcgcaat
     6061 ggggtataac aatggaagct aattttattt ttgcattttt ggattcaatc ttactgcaaa
     6121 ttcctggact taccagtggc ccactcggaa gtgctgatcc tataataaaa ggaatgattc
     6181 catcggcatt tggaattgtt ggtattgcta tttgcctcaa tttgtttaat gccctgataa
     6241 gaagaaaaat ggtagaccaa ggtaaactca aaagactaat gaaagaaaca cgatcttggc
     6301 aaaaagaaag aatggccgca tttaaggcta aagatcaaga caagataaat gaattaaata
     6361 aaaaatctgc ttacatgaac aaaatgaaca tggagcttat gcagatgaat atgagaccaa
     6421 tgatgatcac attcattcct ttgatgttga ttttttattt tgtacttcct cctctatttg
     6481 catacactgt ggcaatatct ccaatgtctc ttaattttgt accagtagac tttttccagc
     6541 tcacgtgcac tgcagaaaaa gtagccgcaa ctccaaacat atgtaaacat gaaaatgaag
     6601 tgtacttttg ggcttggtat tttcttgcat ccatagcttt tagcggcata ataatgcgag
     6661 tcaccaaaac tacaatggat caaaattgag ttgccaaagt cagtaataat ttccggtccg
     6721 ccagcaattg gaaagaccac cgtagcaaaa ggactggcaa aagaatttaa catgaaatat
     6781 cttagcggtg gagatgtgct gaaagattta gcacaagaag aaggatttca aactgaaggc
     6841 gatgattggt gggacagacc agacggaatg aaatttttga atcaaaggaa aaccaatcca
     6901 gaatttgaca aaaaagtaga taataaactc aaggaattgt tttcaaaagg cggtgttgtc
     6961 ataaccagct atacacttcc atggcttgtt aaagatggaa taaagatatg gcttgctgga
     7021 acccaagaaa atagcgcaaa aaggatgact acgagagaca atctttctgc aaaagacgca
     7081 cttgcaattg tgaaaaaaag atatgatgaa aataaaatga tctacaaaaa attatacggc
     7141 tttgattttg gagaaaatct ctccgttttt gataaaataa tagatactga tgatcttgga
     7201 gcagatgaag tacttttagt agcaaaatca gtggtaagaa aattattatg aaactaaagc
     7261 agcttcaaaa tctcaaagta atagatcagg atatcacaaa cgaagaacat ggtacgtatt
     7321 atgacaaaag atcagtagat cagcttctaa actatggatt gatacttctg gataagccaa
     7381 atggtccaac aagccacgaa accgtggctt gggtgaaaaa aatctttaac attcagaaag
     7441 ctgggcatag cggaacactt gatcctcctg tttctgggct cttgccaatt gggtttggtg
     7501 aagcaacaaa ggctttgtct gtcttgttgc ttggaccaaa ggaataccat gcacttggaa
     7561 gattacatac cctgccttca agtgaaaaac ttgattctgt attgagtcaa ttcaaaggaa
     7621 aaattcatca aaaaccgcct caacgttcat ctgtttcacg tcaaactaga atcagaaaca
     7681 tctatgaaat tgaagtcatt gagcagaaag aaaggctctt gcttttgaga atactatgtg
     7741 aagctggaac atacattaga aaaatcttct atgatatggg agaaatattg ggtcctgggg
     7801 caacaatgat cgaattgaga cgaacacgtg ttggacattt tagtgaggat tctaatcttg
     7861 tgaagatgca tgatcttgta gatgcatatg cattatggaa agaaaaagga gatgattcta
     7921 aaatcttacg gataattatg ccaatcgaat acgccctaag tgaaataaaa tcagttgtaa
     7981 ttcgagattc agcagtagat gcattatgtc atggtgcaca gcttgcaata cctggaattt
     8041 tagaaatttc accaaacatc aaaaaaggag attatgtcgg aatatacaca cagaaaggag
     8101 aaatagtcgc tcttggagaa gcgattctat cagaatctga aatcatagaa aacacaaaag
     8161 gatttgcttt tcaaacaaga agaatcatca tggcgccaaa cacttatcca aaaaattggc
     8221 gctcaaaaac aatagttaac aaataaaaag gaaacaaaat taattttcat agttggttcc
     8281 caaaggtgct atcatcggac tctgtgctgg tattactgga gcagtagtac ttggactttt
     8341 cttggcaaat attgccaatc aagttccagt cattgtttat gttgacggtc catcactttc
     8401 tattatgact gaaaagacaa actttgaact aggagaagca gtgacaatta aaataataaa
     8461 ctctggtact gttcctctga ctttttctga ttcttcttat ggcctaaaaa ttgtcgggct
     8521 ggatgggaga attttgtatt ctcctccttc tattcagaca atatcgatac ttcaaccaaa
     8581 cgaagaaaaa acattcgtgt gggatcaaac aaagtctgac ggtgacaaga taattgaagg
     8641 acgctacaag attgtctcaa gcacaatttc tagtggagaa aatgtattga aaaaatctct
     8701 ttcaataaac attttgaaat aattatttat ccaccaaata aggctagcag tgaggttcct
     8761 atggaagaaa gcgcacttgc aaattgtttt actatgtctg agttagtttc actaatagaa
     8821 tcctgttcgt tgtcctctgt aacgtgatgt gagattgatt gcatgtgcgg ttcttcatta
     8881 gttgtaaata ccgttagcga tgtagatgcc ttttctctat tttctagtgt tggtaaatag
     8941 tgtggatttg tctttagtac ttgatcatag tatgatattg cctcttgaaa atttccaaga
     9001 tctgcaagca catttccctt gttgtgtaag gcagctaaat tgtttggatc tatttctaga
     9061 actttatcat aatacaacaa agcgtcatca tatcttccaa gatttgctag agctgaaccc
     9121 gtgttagtta gtgcatgaat attttctggt tcatgttcta gggttctttc ataatgcaag
     9181 atcgcgtctt cgtatttaca aagatgtgca agcgctggtc ctgggctgtt gtattgcgaa
     9241 attacgtcct cgtatgtttt tttctcacaa ttcacgttac tgtaaaaata tgttatcttt
     9301 tcttcctctg gctctaatgg taattcattt ttagcttctg taggtgtgtc ctcagatttt
     9361 tttggatttg tattttcagg tttttttatt tctgaaatgg gtttttgttg tggtggtgaa
     9421 actgtgtttg atgatgtatc tatgtaatca tttagtgaat tttcaattaa taagataaat
     9481 ctctcttcag aaatatttcc ttcaaacaaa tcactgatca tggaacctat tggtaaattg
     9541 ttggccctga tccactttga caaaagattt ttggatatta cagtatttgt gtcagaattt
     9601 aacaaatctt tttttgctag atcttcaaaa aacagcacca aatcatcatc tgtactgcct
     9661 aagtaatacc aagatgccaa gtgttgcaga tctgactctg aaaacagaaa agaatctgtt
     9721 gttggctctt cgatttctgg tgctgaacct gtggattctg ataggcttcc cttctcaaca
     9781 aactctatgg tgagaatttg tttggtttcc agctctccat attgggcagt tacagtatat
     9841 tgcccaataa ctttgaaatt cgtattgtat ttagccaaaa atgaggtaga aaaggttagg
     9901 tctggattca ctgtagcaag ataaatacca gtaggaatgt caaattgatc gaaaatagta
     9961 atggttactt gtggaagaga attttttgat actttccctg taattgaaat taaatctccg
    10021 tcttgatatg tttctttatc agttttaaat aatgtaattt gtggtttcac ttcctctacc
    10081 aagatttcat ccgattcaag aatgtcttca gcctccaaga tctcctcttc tacaaataga
    10141 tcattttcaa tttcaaaaat tatttcagat ttgatcttac catattctgc tattactgtg
    10201 tatatcccag gtttttcata aaatgggatg tcagccttaa ctgtttttga aaaataatta
    10261 ttttcatcaa gctcaacatt gtatcctcca agtatgttgt cttctggatc aaataccttg
    10321 atagctatga gcggcatttt tcttttagtg atttcgcctg agattacaat ggtttcttca
    10381 ggaacgtatg caggcttgtc tgtagaaatt attatatctc ctattggttc gtcttgctct
    10441 tcctcagcta aggcaaaatt ggagttgaga ctgaaaatta agatcgtaaa tatgacaaaa
    10501 aatatgcttt ttttgatcac atgagagcaa atcgcgattt aatatttaat aattaagtag
    10561 aacttgtccg gatgatcttt gtatctaaac ggtacggatt acagatgggg aagacaaagt
    10621 tactacaaac ccaaataccg taatatatag tagattttac aacgacaatg gcgaaattga
    10681 ctgccggggt cgcctagcct ggtagggcgc gcgcctggaa atatctacca taaagcgcgt
    10741 actcgcaagg gttcgagagt tcaaatctct ctcccggcgc cttttttgat aatctcttaa
    10801 agatctcatt ctaattcata ggtgatggca aaatatgatg ttgctataat tggaggtggg
    10861 atacttggca cttctatatc atactggata tcaagccttt ataatgcaaa aatttgtgtg
    10921 attgaaaaag aacctgaggt tgcaatgcat gcaagtagca gaaacactgg cgtattacat
    10981 tctccattct atttagatcc tgtaaaaaag aagatcttgg caaaatctgc ttttatttca
    11041 catgctttgt ggaaaacatt ggcagcgcag aaaaaactac cttggaaaaa tgttggaact
    11101 ctcgaagtgg ctttagatga aaaccaacac aagacgctag aaaagtacat gaaatggggt
    11161 acggaaaatg gtatctcaca gaatgacatt gaattacttg atagcaacga tgtatcaaaa
    11221 agagagccca acgtaagatg tcactctgcg gtgttttgta aaactgatgt tggggtagat
    11281 tacggtgctt ttacgcgtga gataaaagat gaatcacaaa gaaacgggac tcaattcttg
    11341 ttaaaacaca atgtaaaatc tatagacagc aaggaaaagg aatcaacgat aaaatttcag
    11401 gacaatactg aacttacatc aaagctgata ataaactgcg ctggaggaaa ttcacttgat
    11461 attgcaaaaa tggttggaat tgcagaagaa tactctgatc ttcattttag gggagaatat
    11521 tggatagctg atgaccaata tgctgatctt gtgaaaacaa atgtctattc tgttgcaaaa
    11581 ttacctaact ttccttttct tgatccacat tggataagga gagcagacgg tactaccgag
    11641 atagggccta atgctgtgcc tgtcatgagc gcggaaacat attctggata tctaggcgaa
    11701 gcatctacat ctctctcaaa aattgctgaa atagttactg gtaatgcaag aaagctgttg
    11761 ctgaatccag attttatctc tctgatatca aaagaatggt tgagctctat ttcaaaaaca
    11821 gtgatgatcg aaagagtaca gcaattcata ccgaaaatac gctctaattt tttcaacaaa
    11881 cgaggcactg caggaataag aacaccacta attacaccag agggtacgtt tttgcctgat
    11941 gttctagaga tagaaggcgt aaattccttt catgtgttga actataattc tcctggagct
    12001 acaggcgcac cagcttattc tgcatacgtt gtgaaaagac ttcaggaaaa aggactgctg
    12061 aataacgcta cacaaaaaaa atcttctatt tggaattttg aagaaatcat agccaaactt
    12121 aatattttgt ctgcatagat caaaatcaaa ttgttgccag aacactgttc aataaaggaa
    12181 aaaaaccatg attgtccaat gccaccagaa ttcatccttt ccataaaaac taaggaggat
    12241 gaatacatgg tgggagtcgc atgtggagga cataagcaaa cgttttttga aaaattggag
    12301 caattacaaa aagatggcaa aattcctgat ggaacaataa agtttacagg attaaaagca
    12361 gtaggcactg actgtatacg aatggatccc gatgacctca ttcaacttta agatgccttg
    12421 acaatataga ttggattttt acctgttagc gaaagtatca tgtttctgac agttatctcg
    12481 accatttttc ttcttgtttc ctctgtggaa ctgccaatgt gaggagtgag aacaacattt
    12541 ttcattctta ccagtggatg atttttgcct atgggttctt tctgaaatac atctaaagca
    12601 gcaccagcaa taattttttt ctgtaatgca aaaattagat ctttttcttt tatgatcttg
    12661 ccgcgtgcag tattaattaa aaccgcagtt tttttcattc tcttgagaaa ttgtaaattt
    12721 ataatttgat gtgtttcttt tgtataaggc gtatgaatgc taacaatgtc actttcttca
    12781 aataatctct tgaggctgac atatcttatt ccaagatttt tttcttcatt ttttgttagt
    12841 cgatgtctat tataatacaa aatatccatt tcaaatcctt tagctctttt tgctacagct
    12901 tttccaattc tacccatgcc gaaaattcca ataatctttc cagaaagatc acttccaaga
    12961 aaatcatagg atccaaaaat aactttccac ttattttgac gaataattcg atctccttcc
    13021 actactttac ggagcagaga caggatcaat gcaatagtat gatcagctgt agcccttgtc
    13081 aaaacttctg gtgtgtaacc taatacgatc cctttcttgg ctgcatgcct gatgtctata
    13141 tgatcatatc ctacactaaa agtactaatt gctctgagat tctttgctgc atcaataacc
    13201 tctgcatcga ttgtgtcata tggaaagcaa atgaggccat ctttatttct gatttttgat
    13261 attaacttgc tttttggcat ggggatcttt ccatgattaa tctcaatatc ataatgcttt
    13321 cttagctctg aaattgcaaa atctggtagt gtccttgtaa gtaaaacctt caatttcata
    13381 tttctcaata ttaagcctgc tttaagtttg atctggtgtt tcctatgctg aaacaaaagg
    13441 tgttttaaga tacaatgttg atagtatgtt gtatagataa tggaaattat gggaaggggt
    13501 tttaaacaaa ttctacagga gtcgccgtgc tattttcata ttggaatgaa atatgctgca
    13561 tacaagttat ttaacaaacg cagccccttc tatggttctg ctgatataat caacgtctgt
    13621 aatttacact gcacccattg ttattggtgg ttgaacagaa aagaaaacga agaactgaca
    13681 acagaacaat ggaaagagat catcgaacag aaattcaaaa aacaacacgt attcataatc
    13741 acattggttg gcggggaacc gacattaaga cctgacgtca tagaattatt tgtaaaggaa
    13801 ttcccaaaaa gagtttgtat agtttcaaat ggaacatacc cattaaaaaa atatgatgat
    13861 ctctattttt actggatatc tgtagacgga acacaagaaa ttcatgacag tatacgcggt
    13921 aacggtgcat acgcatctac aaggaaaaat atccttgatt atgttgaaaa aaatggtgac
    13981 aaggcatgga aagacatctg gatttctatg accataaatt cactcaatta taaaacggta
    14041 aaaaaagtag cagaggaatg gtacgaatat acaaataaga ttggttttca attccacacc
    14101 ccatttacga aaggggatcc actttggctt ccatttggac cagaacgaga ccaagttgtt
    14161 gatgacatca tagctatgat agacaaatac gacgatcatt atgtaattaa cccaaaaagc
    14221 cagctagaac taatgaagaa aaattggggc ggaagaggta ctaccccact tgattgtccc
    14281 acatgggcaa tactttcagt tgatcacatg ggacgagaaa aactaccatg ttgtataggt
    14341 agtgcagata aagaatctat gaaaccaatc tgcgaggatt gtggtcttgg ttgttactct
    14401 gttcttgtag gttatggaat aagaggctag aatctagcga tataattttt taaaaagcat
    14461 ttataatatt ttgtcttaat actgtcaact tggatcaaga agaatttgct ttgtgcatcc
    14521 aatgtggaaa ttcgttagaa aaatgtattt gtgtttgtcc tttttgcgga gaacgcgaca
    14581 agtgtgaatg tgcacttttt gacgcagcta ctggtggtta atagcatagt attgttaata
    14641 tttgaagcaa ggaaagattc tttgtgaatt cagtagacat aacttgggtt ataggcggcc
    14701 cgcaggggag cggtgtggaa tctgcggcca acatatttgc acgagcttgt gctgcaactg
    14761 gattacagat atttggaaaa cgtgaatttt actctaatat caaaggagag cacagctatt
    14821 tcgcagtcag ggtatcggac aaaaaaattc attctaatgt aagtggaata actttgcttg
    14881 tagcatttga tgctgaaact ttattccgac acgcagacga cgtatatcat gatggtgcaa
    14941 taatttatga ttctgatctt gcacagacaa aagttgatga agtacacaca ttagatgaac
    15001 cattcaagtc acgattggca aaactgcttt cttctaaaaa taaaactgcc tcaattcagg
    15061 gattgcttga tatctgtaaa gataatggag tgcaattaca cccaatttcg tttaaggaca
    15121 ttttatcaaa tctggcagat gagatacaga atcctgctgt aaagaacatg tttaggatgt
    15181 taaacgtatt atgcgtctca gtttcacttg gatttctaga ccttcctcct actacacttc
    15241 tagagtctgt aggatccata ttttctgcca aaccaaaaat agcagaactc aacaagcatg
    15301 cggcaaacta tgcatataat tttgcgacag taaaattccc aaaatccaaa tacgtccttc
    15361 cgtctgttca aaaggaatct aacacacttc ttattcaagg tcatcaagca gcaggtcttg
    15421 gcaaaattgc ctgtggctgc agattccaat catactatcc aattacacct gcatctgacg
    15481 aaagtgtatt tttagaatca aacgaagttt tcgatgtgca agacaataga ccaggttcta
    15541 cacttgtagt acaaacagaa gatgaaattt ctgcaattgg tatggttata ggatcatctc
    15601 ttactggcac tagatctgca acaagtacat caggacctgg attttctcta atggcagaag
    15661 cactcggatg ggctggaata aacgaagttc caattgtagt gacattatat cagcgaagtg
    15721 gaccttcaac gggtctgcca acaagacatg gtcaggatga cttgatgttt gcaatccatg
    15781 caggacatgg agattttcca aaaattgttt atgcatcagg agaagtagaa gaaatttttt
    15841 atgacactgc aaagtgcttc aatttttcag agctttttca agtgccagtc atacatatac
    15901 ttgacaaatt tcttgcaagc acagtgaaaa cctgtaagag atttgatcca aacttgatgg
    15961 atataaacag aggaaaactg cttgaaaaaa tagactctga tgattacaag agatttgcat
    16021 tcacagaaga tggtgtatcc ccacgttcaa aactgggact tgaaaatggt atattttgga
    16081 atacgggaga cgaaagtgac gaacaaggcc acatctcaga agatcctgaa atcagagtca
    16141 aaatgatgga caagcgcgcg tcaaaacttg aacttgccct aaaaacaatc cctttggaag
    16201 atcaagtagt aagctttggg gtgtcagagt tttgcgtggt tagttggggt tctactaaag
    16261 gaccaatcct tgatgcaatg gaaatgctaa aaaaagaagg ataccgtctt ggatttgttc
    16321 aaataaaact tctacagcct ttcccaaaag attatcttaa atcgttactc aaaaactcta
    16381 aagtaataat tgacatagaa gcaaatcatt ctgcccagct tggatcttta ttaaatgaaa
    16441 atttggacaa gaaagtagat tattacattt tgaaatatac tggtagatca atgacaagca
    16501 cagaaattta tcattctctt aaaaaaatac tagaaaaaaa agcagacaaa agacaggtgt
    16561 tgagatatgg agcctaagtt agctgatttt aagaccgaag tccacaatga ttggtgcgcc
    16621 ggttgtggag actttggtat agtaaatgca atacaaatgg cattaacaga gatgaagatt
    16681 ccaagacaca aaactgcatt attttctgga ataggttgct ctggaaaaac ttctcatttt
    16741 gtaaatgtat atggtgttca taccttgcat ggcagagttt tgacatttgc tcaaggtgcc
    16801 aagctcgcaa accctgacat gacagttgtt gcagctggtg gagatggcga tggactagga
    16861 ataggtgcag gacattttgt tgctgcagga agaagaaatg tggacatgac ctacataatt
    16921 ttcaataatg ctgtctatgg tttaacaaaa ggacaagcat cacctacttt acaacttgga
    16981 aaacagacaa aatctctgcc aaacccgaac gtaaaccagg cagtaaatcc gataggacta
    17041 gcaattgcca gtggttttac atttgtggga agaggctatg catatgacgt aagacaactc
    17101 aaggacctta ttgtatctgc agtaaaacac aaaggtcttg catttttgga tgttttgcaa
    17161 ccctgtccaa catacaatga cattaacact agggactggt ttgcaggact ggataacatt
    17221 gatgaagtaa caaagaaatc aagaccacga atttacaaac ttgaagatac tggatatgac
    17281 ccagtagtac actatggttc tgagcaagaa gtcaatgaaa agctaacaca ggcacttgtc
    17341 agatcaaacg aatgggatgc caagattcca actggaattt tctaccaaaa tgagcttgta
    17401 gctccatatg gcgaaagaat ggcagacaga attccaaatt atttagaaaa tccccctgca
    17461 tcacaaccga tagcatctaa tggaaaacca ataactgaca tttcaaagat attggactcc
    17521 cttgaggtct aaaggataaa ttatttacca ctttgatatc tgtaaagaca tgaagatttt
    17581 tgttactggc ggtgcaggtt tcattggtcg ccatctgatt gcatctcttc taaaatcaaa
    17641 taaagtaaca atatatgaca attttaaaaa cagcaccaaa ggaaaaatat ctcatcttgt
    17701 aaaaaatggt gccattatca tacaaggtga tgtaacagat tataagactc tctcgaaatc
    17761 tgtcaaagga tctgatctgg taattcactt ggcggcagaa attgacgttc agaaatcaat
    17821 caaatctcca gaactaattc atcatgtaaa tgtcactgga actgtaaatc tattacgtgc
    17881 atgtgtggca cataaagtat caaatgtcat agctgcctct tctgcggctg tatttggaaa
    17941 tctaaaagat ctaccgcttt ctgaaaattc tccaactgtg ccaatatctc cctatggggc
    18001 aagcaaactg gcaattgaaa gctacatgca ggcattttca catgcatatg atctgaattg
    18061 tattacgtta cgatttttta acatatatgg aaagggacaa tcaaatgcat atgctggtgt
    18121 aattacaaaa tttatggaaa gcattgcaca caataaaccg cttgtaattt ttggtaacgg
    18181 ttccaacaca agagattttg tttcagtgga tgatgttgtg cagtcaattc aaaaagcaat
    18241 tgcaaaaata aaaggtaaaa aaggaaattt ctataacatt ggcagcggga aattcactac
    18301 aattaatgat cttgcaaaac tgatgatatc gctttcagga aaaaatcttt caataaaaca
    18361 caagaaacca aaaaaaggag acatacagca cagccaaacc tcaatttggc tagcacaaag
    18421 agaacttggc tatgttccaa aggtcatgtt aaaggatggg ttggaaaggt tgctagaatc
    18481 tacctcataa aataagttat tagcgggttg agagggagag tagtttatcg ttgaatcttt
    18541 cagaagtaaa caaagtcttt cgtaaatcca taatcaaagg atattttgaa ccacgtcttt
    18601 taagtctgga ttttaaaaaa tcaaatgtaa aacatccatc gattcaggat gatggtctta
    18661 tgcaatcaaa tctgctgtat cttttttttg atattgatac aggaagtgat tatcctgatg
    18721 gtgatgaatg gtttatggca gaatttcttt ttccatacag cataaaaatt cctgatggta
    18781 ttaaaggacc agattacttt agcacgattt ctgttggaga aggaaaaaat ttctggcgac
    18841 acagagaact aatacgatac aaatatggga aatccaaaaa actagaagaa tctcttgatt
    18901 tcattgaaaa aaagta
//
LOCUS       DATIDL010000144         2048 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrosopumilaceae archaeon isolate SMAG_U16342
            k141_177195, whole genome shotgun sequence.
ACCESSION   DATIDL010000144 DATIDL010000000
VERSION     DATIDL010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935836
            Sequence Read Archive: SRR25158326
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosopumilaceae archaeon (soil metagenome)
  ORGANISM  Nitrosopumilaceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrosopumilales;
            Nitrosopumilaceae.
REFERENCE   1  (bases 1 to 2048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/04/2024 05:09:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,520
            CDSs (total)                 :: 1,474
            Genes (coding)               :: 1,474
            CDSs (with protein)          :: 1,474
            Genes (RNA)                  :: 46
            rRNAs                        :: 1 (5S)
            complete rRNAs               :: 1 (5S)
            tRNAs                        :: 43
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2048
                     /organism="Nitrosopumilaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_177195"
                     /isolate="SMAG_U16342"
                     /isolation_source="soil"
                     /db_xref="taxon:2202732"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(173..1168)
                     /locus_tag="VLB45_06475"
     CDS             complement(173..1168)
                     /locus_tag="VLB45_06475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05376.1"
                     /translation="MHSRLQNMLVTIFSCLIIISMVSFVLQDQFANAQEIQIPSWIKS
                     QIKSWVGKPASDGDFVDLLGELNKQGLIKTHDMIKSQNMYLLPKYGQTIFVKIAGRTG
                     DFEQTSPVTLIVVDPKGQRSEYTIPVLESSAYSTVVPISHDSPLGTYTVFAYHTNREL
                     PTSYFYVKADPQIPWWIKDVAKWLTEERISDRDFILSMQYLIDRKIIDLGFMASTDQD
                     LILNVSVDGLKAVRRGTMQSLDVHVSNMAGPVYGAMVFVRVEDYGENILEEFTGVTDS
                     SGNYNLSWELGIDEFNDIETLLVYVDVTDRASSETKLFTFEVYCLCGEPNCKCRN"
     gene            complement(1204..1809)
                     /locus_tag="VLB45_06480"
     CDS             complement(1204..1809)
                     /locus_tag="VLB45_06480"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HSD05377.1"
                     /translation="MSKQAIKFLKRDPKLAKIIKRVGSYEISVMKNPYESLIEAIITQ
                     QLSGKAAESISKKFRKLFVRFPKPADVIKMPDSKLRSAGLSGMKVKYIKDLSEKIESK
                     ELRMRSLKNMTDEEVIMQLTQVKGIGRWTAEMFLIFSLGRLDVLPVGDLGLKKGIQRL
                     YSMPNLPEKDEIEELAEKWRPYRTVATWYIWKSLNQFDKIG"
     gene            complement(1811..>2048)
                     /locus_tag="VLB45_06485"
     CDS             complement(1811..>2048)
                     /locus_tag="VLB45_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010194815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HSD05378.1"
                     /translation="VIVGKSITIIDFESSSLERRVSNVTSATQAILIGSGLAKMVRQI
                     HSIPSKQKIIKALRQYKEDQTQESFDKVLSVLKL"
BASE COUNT          580 a          439 c          348 g          681 t
ORIGIN      
        1 ttttgttatt tggtgtcata gccttttcag tagctgatgt catctactat aatctggagg
       61 caaatgcaag ctatactttg gaacatcctg ttaatttgtt gtggtatgca ggttactgga
      121 tagtgacata cgcattatac aaacacagaa aaattcttta acaaaccata ccctaattac
      181 gacacttgca atttggttcg ccacaaagac aatacacctc aaatgtgaac agttttgtct
      241 ctgaagaagc cctatctgtt acatcaacat acacaagtag agtttctata tcattgaatt
      301 cgtctatgcc caattcccac gatagattgt aattcccact ggaatcggtt acacctgtaa
      361 attcctcaag tatgttttca ccataatcct ctacgcgcac aaaaaccatg gcaccataca
      421 cagggccagc catattacta acatgtacat ccaaagactg catcgttcca cgtctgactg
      481 ccttcaaacc gtccacactc acattaagaa tcaagtcttg atcggtgcta gccataaaac
      541 caagatcaat tattttccta tcaatcaaat actgcattga caagataaaa tctctatcag
      601 agatcctttc ttcagtcaac cattttgcca catctttgat ccaccaagga atttgtggat
      661 cagcttttac ataaaaatag gatgtgggca gctccctgtt agtatgatat gcaaacacag
      721 tgtatgtccc aagtggtgaa tcgtggctaa ttggtaccac ggtagaataa gcactgcttt
      781 ctagaaccgg tatggtgtac tctgatcttt ggccttttgg gtctacaaca atcagagtaa
      841 ctgggcttgt ttgttcaaaa tcgccagttc gtcctgcaat ttttacaaaa atggtttgtc
      901 catattttgg taagagatac atgttttggc ttttgatcat atcatgagtt ttaatcagac
      961 cttgcttgtt tagttcacca agtaaatcta caaaatcccc atctgaagct ggtttgccta
     1021 cccaagactt gatttggctt tttatccaac ttggaatttg aatttcttgg gcattagcaa
     1081 attgatcctg caaaacaaat gataccattg aaattatgat tagacaagaa aatatggtga
     1141 caagcatgtt ctgcaatcta gaatgcaata attgctttca taataataaa aagttcttcc
     1201 aactcatcca attttatcaa actgattgag gctcttccaa atataccacg tagcaactgt
     1261 cctgtacggc cgccattttt cagccagctc ttcaatttcg tccttttctg ggagattagg
     1321 catagaatac agcctctgta ttcctttttt gagacccaga tctcctacag gtaaaacgtc
     1381 caatctgcct agcgaaaaga tcaaaaacat ttctgctgtc cagcggccaa ttccttttac
     1441 ttgggtaagc tgcattatca cttcctcatc tgtcatattt tttagcgaac gcatcctcag
     1501 ctcttttgat tcgatctttt ctgacagatc ttttatgtat ttcaccttca tgcctgacag
     1561 cccagctgaa cgtagcttgg agtcgggcat tttgatcaca tctgctggtt ttgggaatct
     1621 gacaaataat tttctgaatt tctttgaaat ggattccgct gcttttcctg agagttgttg
     1681 cgtaataatc gcttcaatca aagattcgta cggatttttc ataacgctga tttcatagct
     1741 tccaacacgc ttgattattt ttgcaagctt cggatccctt tttagaaatt ttattgcttg
     1801 ttttgacata ttacagcttt agaactgaga ggactttatc aaaactttcc tgcgtttgat
     1861 cttctttata ttgtcgtaat gctttgatga tcttttgttt tgatggaata ctgtgaattt
     1921 gccgaaccat ttttgcaagg ccagagccaa taaggattgc ctgcgttgca gaagtcacat
     1981 ttgaaaccct tcgctcaaga ctagagcttt caaaatcaat tattgtaatt gattttccta
     2041 caatgaca
//
LOCUS       DATIDL010000145        15032 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrosopumilaceae archaeon isolate SMAG_U16342
            k141_2198184, whole genome shotgun sequence.
ACCESSION   DATIDL010000145 DATIDL010000000
VERSION     DATIDL010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935836
            Sequence Read Archive: SRR25158326
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosopumilaceae archaeon (soil metagenome)
  ORGANISM  Nitrosopumilaceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrosopumilales;
            Nitrosopumilaceae.
REFERENCE   1  (bases 1 to 15032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/04/2024 05:09:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,520
            CDSs (total)                 :: 1,474
            Genes (coding)               :: 1,474
            CDSs (with protein)          :: 1,474
            Genes (RNA)                  :: 46
            rRNAs                        :: 1 (5S)
            complete rRNAs               :: 1 (5S)
            tRNAs                        :: 43
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15032
                     /organism="Nitrosopumilaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2198184"
                     /isolate="SMAG_U16342"
                     /isolation_source="soil"
                     /db_xref="taxon:2202732"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..221)
                     /locus_tag="VLB45_06490"
     CDS             complement(<1..221)
                     /locus_tag="VLB45_06490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05379.1"
                     /translation="MENPFYVEKHMFDEKPIVTFLDDAYNLSYSHKTRELYKQILHSF
                     NDFCLIQYDKNMLQIIHGLREKPLHEVLEV"
     gene            317..1072
                     /locus_tag="VLB45_06495"
     CDS             317..1072
                     /locus_tag="VLB45_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HSD05380.1"
                     /translation="MSWLTDIHIHLSDPEYSSDLPFIIKSMERMKIRACCMSMDLSSS
                     QKTLDLAKRTPLVLPFLGIHPEKAEDDLDAVTKLIEKNSKQISGIGEIGLDKTYVTTK
                     EGLEQQEKVFRAMLSIAEKLHKPISIHSRATLDEILAILPSYSISATLLHWFAGSKKQ
                     LKTVMDRGYYVSYGPAMVYAQDKQILLSETNHDRILVETDGPVRFSRCFGLKTAQITF
                     LPSVVFCAAKILHKTYDEVSDEIEQNAKRYLGV"
     gene            1104..1367
                     /locus_tag="VLB45_06500"
     CDS             1104..1367
                     /locus_tag="VLB45_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010191085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05381.1"
                     /translation="MNRIKRISMEVLAIHKAKFGTDFAQNKKILNEISIVRSKGLKNE
                     LAGYITTYIKREIEDQKEKEAQAIQAAQSPEETEGMHEEEILN"
     gene            1433..2209
                     /gene="cgi121"
                     /locus_tag="VLB45_06505"
     CDS             1433..2209
                     /gene="cgi121"
                     /locus_tag="VLB45_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KEOPS complex subunit Cgi121"
                     /protein_id="HSD05382.1"
                     /translation="MITVKLLGGAKKSLSADKIEFEKDDSTVSELLDLLQNKIEKNMP
                     PLDMENMLVAVNGVDSSALDGFATKLKNGDIVTIIPVIHGGSTKRIRFEISNKTIDLL
                     EIKAIDDPIEFLDGLRKKYHDLAIQGIGSYYVLNAEHARKIILISLEAQKSGVMLSNK
                     IETDLLMRFACTKQIGKAIQKVGLKKKQDFILISIGKKSSLDKLYDELKTSLKPKQLS
                     RNNSTFLKKEFEITKKHFDAIASKTPLEDLLAEKAAVLFR"
     gene            complement(2198..2719)
                     /locus_tag="VLB45_06510"
     CDS             complement(2198..2719)
                     /locus_tag="VLB45_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
                     /protein_id="HSD05383.1"
                     /translation="MNLIITCSRHLESEAAQEIHDILEELGDDDSTVDITHMSGIVTA
                     VTSLDPFVVVEKIKKRILDEPWSIRYCLRIIPIQETVATEKEEIVNAVIKHAAIIESK
                     DTYRITVEKRHSAISTSEIISGIADKIHSKVSLEQFEWIILVEILGNKTGISVLKEAN
                     IVSTQKLKRSLSE"
     gene            complement(2716..3318)
                     /locus_tag="VLB45_06515"
     CDS             complement(2716..3318)
                     /locus_tag="VLB45_06515"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HSD05384.1"
                     /translation="MIVACDQNGIDLAAATIRNGGTVVFPTDTVYGVGCDPRNPKAIQ
                     SIFKLKKRVEAKQLPILGYSKEEIAKIAIFDEASSKIADRFWPGQVTMVLKLIDMKIQ
                     NAMGIKDGKIAVRVPNQPCVLSLLSKCKILIGTSANFSGLPAFSESKKVQENFSGYDV
                     FLDGGDIPNSTSSTIVEVIEGNPRVLRQGKISKEEIASVL"
     gene            complement(3747..4037)
                     /locus_tag="VLB45_06520"
     CDS             complement(3747..4037)
                     /locus_tag="VLB45_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HSD05385.1"
                     /translation="MAAVYRTHMSIIGDILSTARDEAPDETGASVTYLIRKANVSHGR
                     ISKILVTLVQQGLLEQTSSDRACKYKISPRGREFLQAYRGFREFADNFGLRI"
     gene            complement(4021..4656)
                     /locus_tag="VLB45_06525"
     CDS             complement(4021..4656)
                     /locus_tag="VLB45_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05386.1"
                     /translation="MQFAYEIILNLIGFLVGLTIGILSNIGFRNTGSPTLFRLTIAFL
                     SISIGFGVVGLGYLTESFVFQTGKIEPWVQTLGIGIQTVGYFFIAFSHSIKSFFPKSR
                     YFRSIILPLFVIPGNSIEHIIRSISFILLIYGTIETILSYMENKKSSTVFVASGLGLL
                     ALGEFLGWYSLVFPGSVLYFVSISTKIAGLGALFVPVSKIPLMGMKFGGSL"
     gene            complement(4708..4914)
                     /locus_tag="VLB45_06530"
     CDS             complement(4708..4914)
                     /locus_tag="VLB45_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05387.1"
                     /translation="MKIKCPKCDGVAELGDDFSFVRCEPCSLDMTYGEYVKYIAYKDS
                     TYSDILGDYATSTEGTTAGTLDEW"
     gene            4985..5260
                     /locus_tag="VLB45_06535"
     CDS             4985..5260
                     /locus_tag="VLB45_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron transporter"
                     /protein_id="HSD05388.1"
                     /translation="MAKICTKCKNEIPDDMELEPVAANYPCCSTCWNEWKNYRVMVMN
                     EMKLDMSLSDHRKVLKKYEKIFVGVMTPDGNVVNFADENQRKPDHPV"
     gene            complement(5257..5718)
                     /locus_tag="VLB45_06540"
     CDS             complement(5257..5718)
                     /locus_tag="VLB45_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010191072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05389.1"
                     /translation="MSEPNYAGNIIVSLASLPDFLRKPILQKRLMEFFSMSDQDRSET
                     INNALAAGPTIPFDNFAKLFKTWLEILSGLSEEHRTIMFSSYLTEITTNPQKLILFNL
                     DGILEIFLLLTQTQKDTISNSVKKIVNTLDESSKKKLRLIIPDRARQEFGI"
     gene            5797..6795
                     /locus_tag="VLB45_06545"
     CDS             5797..6795
                     /locus_tag="VLB45_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HSD05390.1"
                     /translation="MPSTVVVGGFFGDEGKGKIISYLAIKDNPTIAVRGGAGPNAGHT
                     IELDNKKYKVRMLPSAFLNKNVRLLIGPGVVVSPEVLLKEIAEFDVSDRAYVDFNCGI
                     IEESHRNADSQGRLKESIGSTGSGTGPANADRAMRTLKLAKDIDSLALYLDDVPNAVN
                     FALQRNETVLVEGTQGTFLSLWHGTYPFVTSKDVTASGICSDIGLGPKNVTDVMVVFK
                     AYVTRVGTGPLKNELSAEQTKIKGWQEVGTVTGRARRAAEFDFDLAKRSIMLNSATQI
                     AITKLDVLYPECAHMNSFSDLSSPAKHFVNTIEEQLKVPVTIIGTGPDVNDIVDRR"
     gene            6847..7500
                     /locus_tag="VLB45_06550"
     CDS             6847..7500
                     /locus_tag="VLB45_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007551268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05391.1"
                     /translation="MAKLPTYVEVADRIEFSRLVCALERVPRTAFSFEHEGKPVLAVQ
                     MDLLKERPVIYYAPAEKSGHYISYGFKTGKEESDIVNTITNSTFLYSPIVRVKSLPPS
                     LKQDDKPQDLSYELLELEDLASLVKLSYGFEEAPFPLFAFSNGSKWLIGVFMNFNESD
                     EVSYFCHVTLDKEPVNPFLKYSSQNGVEPSFTPTVSEHGYSYLKVIKLKGKHPLVKL"
     gene            7497..8246
                     /locus_tag="VLB45_06555"
     CDS             7497..8246
                     /locus_tag="VLB45_06555"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016939459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine 5'-phosphate decarboxylase / HUMPS
                     family protein"
                     /protein_id="HSD05392.1"
                     /translation="MNYRNRIKKSSSGKSKIILANDLDKTPSKNIESVTIKNIKTLNR
                     FLCAIKFNFHVILPLGIKQLEKINQAAHDVGLQTIADIKLNDIGNTNMVAAERLWESG
                     FDAIIVNPIMGPENLENLIISSHKQNNGIISLVHMSHPGAKLGYGISIKPTSSKKLSK
                     LHELFLDWSYSLRTDGIVVGATVPEIIKSCSKKVKGRCEIYSPGVGTQGGDATKAIRS
                     GSDYLIVGRTILNAKDPIKEAKKLQELSIES"
     gene            complement(8229..8645)
                     /locus_tag="VLB45_06560"
     CDS             complement(8229..8645)
                     /locus_tag="VLB45_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HSD05393.1"
                     /translation="MIEERSAGAVLFAETSSGKKFLLLNYPSGHWDFVKGNIEKGENL
                     RDTVIREIREETSITDVEFIDGFEDKIEYHYQRNGELVHKEVVFFLAKTKTLDVKISH
                     EHLGFVWLAFDDALKKMTYKNAKNTMEKVRKLGLDA"
     gene            complement(8684..9451)
                     /gene="uppS"
                     /locus_tag="VLB45_06565"
     CDS             complement(8684..9451)
                     /gene="uppS"
                     /locus_tag="VLB45_06565"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007551264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HSD05394.1"
                     /translation="MNFSGLYKIYSNKLEKEIRNGNLPNHIAIILDGNRRWAKRNLIV
                     KEDGHFRGADAVENLLDWCEELGIKIITLYVLSAENLSRDDDELHYLYDLIKTRLEKL
                     YKDPRIHRNRMRVKAIGRVELLPESLKDVLNRLDQATINYDNHYLNIAIAYGGQHELV
                     DAVKKIAHRIKDGSLDVDSIDKEVIESSLYTSHLPQASPDMILRTSGEKRLSGFLIWQ
                     SAYSELVFMDVFWPEFRKIDLMRAIRTFQKRTRRLGK"
     gene            complement(9463..10605)
                     /locus_tag="VLB45_06570"
     CDS             complement(9463..10605)
                     /locus_tag="VLB45_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF373 family protein"
                     /protein_id="HSD05395.1"
                     /translation="MSVNENLEKKVTETKTSKLLVICVDRDDDIGKKAGVTTPVIGRN
                     ACIEAAQRLALEDPEDADSNSIFAAVKTYEDLVSKGYQAEVIVVAGTESRGVQADEKI
                     VGQVKTIVEQYRATGAVIVSDGEDDESVIPIIQNVLPIVSVRRVVMRVSRSVEYSYAV
                     LGRYLRAIAFDSKYSKFFLGVPGILLLIGGVASVLGITKEIFAVLVSILGGAFLIRAF
                     DVDRAWSHWTKPTPAGFIRIFTMVAGALLMLVSIPSGISAVNPSLIQAQPDIVSIVTN
                     KVIIGLYVSGALPFLWMGMGCVFGGTLLSSWFKGSIRSVTDILRIIVLVTLYPVVLQF
                     TNIMTHDDSPFTLLPPLLLGLAVILIAATILFRKYRKKKGGEVLSE"
     gene            complement(10703..11197)
                     /locus_tag="VLB45_06575"
     CDS             complement(10703..11197)
                     /locus_tag="VLB45_06575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05396.1"
                     /translation="MTKKRSIIITGAILAAITAASFSVWLIPQGDDSSFVVSDYRDHL
                     ESVKARHFVITDEIQNDLQAMLAGTVSPDNFIMRAEASSSQINSLIIELIQSGASQEW
                     HESYLNYGESLKSYNSYLRETIVLANIIKERGANDIQGNLETLDALKKEFESFSLKSD
                     ETRP"
     gene            11452..11595
                     /locus_tag="VLB45_06580"
     CDS             11452..11595
                     /locus_tag="VLB45_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007551261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5679 domain-containing protein"
                     /protein_id="HSD05397.1"
                     /translation="MTQAYCVKCRKKVDISNPKEVKLKNGRPAVKGTCPKCGTNVFRI
                     GKP"
     gene            11655..12077
                     /locus_tag="VLB45_06585"
     CDS             11655..12077
                     /locus_tag="VLB45_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 subunit beta"
                     /protein_id="HSD05398.1"
                     /translation="MAKSEYEKLLKKIQDKISENKKDSVSRFELPIVDIMWQGNRTFF
                     RNFAEFPKILRRDPDKVLQYLSKEFASPAQTAGDKAIFVGKKDPHDFTALFARYVKEY
                     LECPTCKSPDTRVEKSNRMTFLICEACGAKSSLKGQYA"
     gene            12077..12373
                     /locus_tag="VLB45_06590"
     CDS             12077..12373
                     /locus_tag="VLB45_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF424 domain-containing protein"
                     /protein_id="HSD05399.1"
                     /translation="MSFAVRTVAYQQHLMLNICDANLVGKTITKSDFSLNISSSYFAE
                     RFVEKTEAEELLKKYSIINMVGKETIALAIELRIGSSKGVKEIEGVPFLLVFKF"
     gene            12423..12734
                     /gene="eif1A"
                     /locus_tag="VLB45_06595"
     CDS             12423..12734
                     /gene="eif1A"
                     /locus_tag="VLB45_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor eIF-1A"
                     /protein_id="HSD05400.1"
                     /translation="MGKRKVLNESELKEMKLPEEGELLGRVIKLLGSDHVLVKCTDDK
                     TRMGRIRGKLKRKIWIRDNDVVTLAPWDFRTEDRGDITWRYTLSQVDWLKDNGHLPKD
                     F"
     gene            12775..13557
                     /locus_tag="VLB45_06600"
     CDS             12775..13557
                     /locus_tag="VLB45_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protein kinase RIO"
                     /protein_id="HSD05401.1"
                     /translation="MSDELGKKLANRIDREVLANERRGRSEKGVLDKIKVMDDVLDKT
                     TIMTLSKLINSGVISYVNGVVGSGKESKMYWAVDPDGKDLALKIYLVTAANFKKRLPY
                     LVDDPRFSHIKKGTRNLVELWAQKEFRNLNHCIKSGIPVIKPIHVIKNILVLEFVGKN
                     GAPAKTLVETEIDENDYKDAISIISQLYKKAKLVHADFSEYNIFKTENGLILFDLGSA
                     VDVRHKNAKEFLERDIKNITRFFVKRGLTVENPSDIFARIVN"
     gene            13554..14126
                     /locus_tag="VLB45_06605"
     CDS             13554..14126
                     /locus_tag="VLB45_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HSD05402.1"
                     /translation="MSFEKSVLIPLDRIGALIGKSGKVKSRIEKTCSVKIVVNSETGE
                     IMVSGVGSIEDMLPFKAVEIVMTIGRGFSPEKAMRLLQGENALHVIDLREFAGRSSSN
                     VERIKGRIIGEGGRVRQNIEELSGANISVYGRTVSIIGEGNQLRNAVDAISALSSGSM
                     HGAVYNELQEARRREKLDRLRLWENSNVFD"
     gene            14113..>15032
                     /locus_tag="VLB45_06610"
     CDS             14113..>15032
                     /locus_tag="VLB45_06610"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase VI subunit B"
                     /protein_id="HSD05403.1"
                     /translation="MSSTKEIFSQISPSEFFYRNRDLAGFSNPTRSLYTSVREFVENA
                     LDACDQKGILPDITLTIKAVDPEQADPKPYILSVKDNGPGIEPKHVPLAFGTVLYGSK
                     FGLKQARGMFGLGATMAILYGQITTNKPVTVKSCADGKTQDEFVMLLDIQKNKPIIQK
                     HVTKEASKTGLSVSIVLEGDYSKAGSKIRDYVLQTSLITPYASITFEEPTGEPHKYPR
                     IVKEMPPPPTIIKPHPHGIDVETIRRMMVDTHYQIPVVDNKMITKVRSELGLQRKNLN
                     AKGILERAEKRWSNLSRPVRIVIAMMSFLNL"
BASE COUNT         4960 a         2784 c         2737 g         4551 t
ORIGIN      
        1 acttctagaa cttcgtgtaa tggtttttct cttaaaccat gtattatctg aagcatgttc
       61 ttgtcgtact gtatcagaca aaaatcattg aaagaatgaa gaatctgttt atacagctcc
      121 cgtgtcttgt gagaatagct aagattgtac gcatcatcca agaatgtgac aataggtttt
      181 tcatcaaaca tgtgtttctc aacataaaat ggattttcca cgccagaaaa agcaaggaac
      241 tcaaactaat aaaatacata cagtacaggc aaaatttcca ttctaactat tttaaggaga
      301 aaaataaaga cctgccatga gctggttaac tgacatccac attcatctct cagatccaga
      361 gtattctagt gatctaccat ttattattaa aagcatggag cgcatgaaga tacgagcatg
      421 ctgtatgtcg atggatcttt caagttccca aaaaacactt gatcttgcta aaagaacccc
      481 acttgttcta ccatttcttg gaatacatcc agaaaaggct gaagatgatc tagatgctgt
      541 tacaaaattg atagaaaaaa actcaaaaca aatttctggc attggagaga ttggtcttga
      601 caaaacatat gtgactacta aagagggact tgagcaacaa gaaaaagtat ttcgtgccat
      661 gctttcaatc gcagaaaaat tacataaacc aatttccatt cattcgcgcg ctactcttga
      721 tgagatcttg gcaatcttgc cttcgtattc aatttctgca acccttctgc attggtttgc
      781 aggcagcaaa aaacaattga aaactgtaat ggatcgtgga tattatgtct cttatggtcc
      841 tgcaatggtt tatgcacaag acaagcaaat tctactttca gaaacaaacc acgatagaat
      901 acttgtggaa acagacggac ctgtaagatt ttctagatgc tttggattga aaactgcaca
      961 aatcactttt cttccaagtg ttgttttttg cgcagccaag attctacata aaacatacga
     1021 tgaagtttca gatgagatag aacaaaatgc caagcgctat cttggtgtat aggtataaaa
     1081 accccataca tccaatcgaa tccgtgaaca gaatcaaacg aatttcgatg gaagtactag
     1141 ctatacataa agcgaaattt ggaactgatt ttgcacaaaa caaaaagatc cttaatgaaa
     1201 tatctatagt tagatcaaaa ggactcaaaa acgagcttgc aggctacatt actacctaca
     1261 taaagcgaga gattgaagac cagaaagaaa aagaggctca ggcaatacag gcagcccaat
     1321 ctccagaaga aacagaagga atgcatgaag aagagatact aaactagaca tattttctaa
     1381 aaacctcggt caaacagaaa ttcaatatat ggaaaaaatt acttttggct agatgataac
     1441 agtaaaactc ttgggcggtg caaaaaagtc cttatctgca gacaaaatag agtttgaaaa
     1501 agatgattct actgtgtctg aattgctgga tttacttcaa aataaaattg aaaaaaatat
     1561 gccgccttta gatatggaaa atatgttggt tgctgtaaat ggagttgatt cttcagctct
     1621 tgacggattt gcaacgaagt taaaaaatgg agatattgta acaataattc cagtaataca
     1681 tgggggaagc acaaaacgaa tacgttttga aatatctaac aaaacaatag atcttttaga
     1741 aataaaagca attgatgatc caatagaatt ccttgatggt ctacgaaaaa aatatcatga
     1801 tttagctata caaggtatag gatcatacta tgttttaaat gcagaacacg ctagaaaaat
     1861 aattttgatt tctttagagg cacaaaaatc tggagtcatg ctctcaaaca aaattgagac
     1921 tgaccttttg atgagatttg catgtacaaa acagataggc aaggcaattc agaaggttgg
     1981 attgaaaaag aaacaagatt ttatcttgat atctatagga aaaaaatcgt cacttgataa
     2041 attatatgac gaactcaaga ccagtttgaa accaaaacaa ctttctagaa ataattctac
     2101 tttccttaaa aaagaatttg aaataaccaa aaaacacttt gatgccatag catcaaaaac
     2161 tcctcttgag gatctgcttg cagaaaaggc agccgtacta ttcagataag ctacgcttta
     2221 gtttctgcgt acttacaatg ttggcttctt ttaataccga tattcctgtt ttatttccaa
     2281 gtatttctac tagtataatc cactcaaatt gttcaagtga tactttgctg tgaatcttgt
     2341 ctgctattcc acttatgatc tcactagttg aaattgcaga gtgtctcttt tctactgtaa
     2401 ttctgtaggt gtccttagat tctattattg cggcatgctt gatgacagcg tttacaattt
     2461 cttctttttc cgtagctact gtttcttgaa tcggtatgat tctaagacaa tatcttatac
     2521 tccatggctc gtcaagtatt ctttttttga tcttttctac tacaacaaat ggatcaagtg
     2581 aagttacggc agtcacaatt cctgacatgt gggttatgtc aactgtagaa tcatcatctc
     2641 caagctcttc tagaatgtca tgtatttcct gagctgcttc agactctaaa tgtctgctac
     2701 atgttataat caggttcaaa gtacggaagc aatctcctct ttacttattt ttccttgtcg
     2761 taaaactctc gggttgcctt ctatcacttc aactatggtg gaactagttg aatttggaat
     2821 gtctcctccg tctaggaaca catcatatcc tgaaaaattt tcttgcactt tctttgattc
     2881 tgaaaaagca ggaagtccag aaaaatttgc actggtacca attaggatct tacatttgct
     2941 taaaagtgat agaacacacg gctgatttgg tacacgaaca gctattttgc cgtcctttat
     3001 ccccatggcg ttttgtattt tcatatctat taattttaaa accatagtga cttggcctgg
     3061 ccaaaatcta tctgcgattt tactagatgc ctcgtcaaaa atagctattt tcgcaatttc
     3121 ttcctttgaa tatcctagta tgggaagttg tttggcttct actctttttt ttagcttgaa
     3181 aatagattgg atagcttttg gatttctagg atcacatccg acaccatata cagtgtctgt
     3241 tggaaatacc actgtgccac catttcttat tgtagcagca gcaagatcta tgccattctg
     3301 atcgcaagca actatcacat ttttttcaaa tcttgttagt aaattatgtc ttgttgttga
     3361 aaatgatata agattgattt acgtagtatc tggcatggat gaagaaattc accagtgtca
     3421 atgtccaccg ggttctgaga cttatgtcaa cgaggatgga atggacatct gtgtttcttg
     3481 tggtggctgg gtagtacctt cataagaata acttgactca aaatcaaata ctagtataga
     3541 agctgttata gaatattgta tgaaataaaa atttgaaaaa tcacttgctt ctaaaatatg
     3601 tcgtttttct atgattatac accaactaaa tccttaataa ttaatcacat ttgtcttaca
     3661 accaatgtct gaagaacgtg ataatgattt tgaagacgat ttcgatgatg atgtagaaga
     3721 cgactcggaa gtagaagatg actagactaa attcttagac caaaattgtc tgcaaattcc
     3781 ctaaatccac gataggcttg taaaaattct cttccccttg gactgatttt gtatttgcat
     3841 gccctgtcag aacttgtttg ttctaacaag ccttgttgta ccaaagttac tagtattttt
     3901 gagattcttc catgagaaac atttgctttg cgtatcagat atgtgacact tgcaccagtc
     3961 tcatcaggag cctcatctct agcagtagat agtatgtccc cgattatact catgtgtgtt
     4021 ctatagactg ccgccaaatt tcatccccat caatggtatt tttgaaacgg gtacgaacag
     4081 tgcacccaaa ccagctattt tagtagaaat tgagacaaag taaagaactg aaccaggaaa
     4141 gacaagcgaa taccatccaa gaaattcgcc taacgcgaga aggcctaggc ctgaagcaac
     4201 gaaaaccgta ctactcttct tgttctccat atatgacaga attgtctcta ttgtcccata
     4261 tatcaatagg ataaaagaaa ttgacctaat aatatgctca attgaatttc cgggtattac
     4321 aaaaagcggc aaaattatgg atctaaaata tcgtgatttt gggaaaaatg attttatgct
     4381 gtgagaaaaa gcgataaaaa aatatccaac tgtttgaatg cctattccaa gtgtctgaac
     4441 ccatggttct atttttcctg tctggaaaac aaaagactct gtaagatatc ccaatcctac
     4501 tactccaaaa cctatgctaa ttgacaaaaa agcaatagtt agtctaaaaa gagtaggact
     4561 gccggtgttc ctaaagccaa tgttagataa aatccctatc gtaaggccta ccagaaagcc
     4621 tatcaagtta agaattatct catatgcgaa ttgcaactaa tcgcagtatc ctcggaaatt
     4681 aattaaatct tgcaggtggt tttttttcta ccactcgtca agagttccgg ctgtagtgcc
     4741 ttctgtgctg gtagcataat cacctagaat atctgaataa gtagaatcct tgtatgcaat
     4801 gtactttaca tattcaccat atgtcatatc caatgagcac ggctcacatc taacaaaaga
     4861 aaagtcgtct ccaagctctg ctacaccatc gcattttgga cactttatct tcataacata
     4921 tctagccttc ccaacgtaat atgctttctt aaagattaaa tgagtaacag ctagtgaatt
     4981 tcgtatggca aagatatgca caaaatgtaa aaatgaaatt cctgatgaca tggaacttga
     5041 acctgtggct gcaaattacc catgttgtag tacgtgttgg aatgaatgga aaaactatcg
     5101 agtcatggtc atgaatgaaa tgaagcttga catgtcactg tctgatcata ggaaagttct
     5161 aaaaaaatat gagaagatct ttgttggtgt tatgactcca gatggaaatg tggtcaattt
     5221 tgctgatgaa aatcagagaa aaccagatca tccagtttaa attccaaatt cttgccttgc
     5281 cctatctggt attataagtc gcaatttctt cttactggac tcatctaaag tattaactat
     5341 ttttttaacg gaatttgata ttgtatcttt ctgggtttgt gtgagcaaaa gaaatatctc
     5401 taaaatgcca tccagattaa acagaataag cttttgtgga tttgtggtga tctctgtcaa
     5461 gtagcttgaa aacatgattg ttctgtgttc ttctgaaagt ccacttaaaa tctcaagcca
     5521 tgttttaaac agctttgcaa agttgtcaaa cggaattgtt ggacctgcag ccagtgcatt
     5581 gtttatggtt tcagatctgt cttgatcaga cattgaaaaa aattccatca gtcttttttg
     5641 taaaattggt tttcttaaaa agtcagggag acttgctaat gatactatga tatttcctgc
     5701 ataatttggt tctgacacaa aggctgcact gactttcttt attaaaattg tattgtcata
     5761 atttggctta aatatgcgct cttgtgcaat tcttgtatgc cttctacagt agttgttggt
     5821 ggtttctttg gtgatgaagg aaaaggaaaa ataatttcat atcttgcaat aaaagataat
     5881 cctacaattg cagtacgagg aggtgcaggc ccaaacgcgg gtcacacaat agaacttgac
     5941 aataaaaaat acaaagttag aatgctgcca agtgcgtttt taaacaaaaa tgttaggctt
     6001 ttgattggac cgggtgttgt agtcagtcct gaagtccttc taaaagagat agcagaattt
     6061 gacgtttctg acagggcata tgttgatttt aactgtggaa taattgaaga atcgcacaga
     6121 aacgctgatt ctcaaggtag gctgaaggag tctattggca gtacgggatc aggtacagga
     6181 cctgctaatg cagacagagc tatgcgaacg ctgaaacttg caaaagatat tgattctctt
     6241 gctctgtatc ttgacgatgt tccaaatgct gttaattttg ctttacaaag aaatgagact
     6301 gttctagtgg aaggaactca aggcaccttc ctttccttat ggcatggcac gtatccattt
     6361 gtcacgtcaa aagatgttac tgcatctgga atatgctctg atattgggct aggaccaaaa
     6421 aatgtaactg atgtcatggt agttttcaaa gcatatgtga ctcgagttgg tacaggtcct
     6481 ctaaaaaatg aactttcggc agaacaaact aaaatcaagg gttggcaaga agttggcact
     6541 gtcactggcc gtgcaagacg ggcagcagag tttgattttg atctggcaaa gagatctatc
     6601 atgttaaaca gcgcaaccca gattgctatt acaaaacttg atgtcctgta tccggaatgt
     6661 gcacacatga attcgtttag tgatctaagc tctcctgcaa aacactttgt taatacaatt
     6721 gaagaacagc tcaaagtgcc tgttaccatc ataggtactg gtcctgatgt caatgacatc
     6781 gttgaccgaa gataacgatt gaatcaagtt gctacctttt aatggggtat tttttgcaag
     6841 aaaactatgg caaaattacc tacttatgtt gaagttgcag atagaattga attctcaagg
     6901 cttgtgtgtg ctttagaaag agttccacgt accgccttct catttgagca tgagggcaag
     6961 cctgttttgg ccgtacaaat ggatcttcta aaagaaagac cagtcatcta ttatgctccc
     7021 gctgaaaaaa gtggtcacta tatctcatat ggttttaaaa ctggcaagga agaatccgat
     7081 attgtaaata ccattacaaa ttctactttt ctttactctc caatagtgag agtaaaatct
     7141 ctacctcctt cgctaaaaca agatgacaaa ccccaggatc tgagttatga actacttgaa
     7201 cttgaggatc ttgcaagtct ggtaaagcta agttatggat ttgaggaagc tccatttcca
     7261 ctctttgcct tttcaaatgg aagcaaatgg ctaattggcg tctttatgaa ttttaacgag
     7321 tccgacgaag tatcatactt ttgtcatgtg acactagaca aggaaccagt aaatccattt
     7381 ttgaaatact ctagtcagaa tggagtagaa ccgtcattta ctccgactgt aagtgaacat
     7441 ggatattcct atcttaaagt aataaaacta aaaggcaaac atcctcttgt taagctatga
     7501 attatagaaa taggataaaa aaatcatctt ctggtaaaag caaaataata ttagcaaatg
     7561 atcttgataa aacaccttca aaaaatattg aaagcgtcac aattaaaaac ataaagacat
     7621 taaacaggtt tctttgcgca atcaaattca attttcatgt aattttgcct cttggcataa
     7681 aacaacttga aaaaataaat caagctgcac atgatgtagg cctgcaaact attgctgata
     7741 taaaattaaa tgatattgga aatacaaaca tggttgcagc agaaagactt tgggagtcag
     7801 gttttgatgc aataatcgtg aatcctataa tggggccaga aaaccttgaa aacctcatca
     7861 tatcctctca caaacaaaat aatggtataa tctcactcgt acacatgagt catcctggcg
     7921 caaaactagg atacggcata tcgataaagc caacatcaag taaaaaatta tcaaaattgc
     7981 atgaactgtt cttggattgg tcgtattctc taagaactga tggtatagta gttggagcta
     8041 cagttcctga aataataaaa tcatgtagca aaaaagtaaa aggacgatgt gaaatttact
     8101 cgccaggagt tggtactcaa ggtggtgatg caaccaaagc aatacgaagt ggttcagatt
     8161 atcttattgt aggcagaact attttgaatg caaaagatcc aataaaagaa gctaaaaaac
     8221 tacaagagct aagcatcgag tcctagcttt ctaacttttt ccattgtgtt cttggcattt
     8281 ttataggtca tttttttaag tgcgtcatca aaagcaagcc atacaaaacc taggtgttca
     8341 tgtgagatct ttacatcaag tgtttttgtc tttgcaagaa aaaaaacaac ttccttgtga
     8401 accaactcgc cattccgttg ataatgatat tctatcttgt cttcaaagcc gtcaataaac
     8461 tccacatctg taatgctggt ctcctccctg atctctcgta ttacggtatc gcgaagattc
     8521 tctccttttt cgatattgcc ttttacaaaa tcccagtgcc cagagggata attcaaaaga
     8581 agaaactttt ttccagaact ggtttcagca aataacactg ctcctgcaga tctttcttca
     8641 atcacaatct agaaaaaatt gttcagctaa gtatttcttt ctactatttg cctaatcttc
     8701 tagttctctt ttggaatgtc cttattgctc tcatcaagtc tatttttcga aattctggcc
     8761 aaaaaacatc catgaataca agttcactat aggcactctg ccaaatcaga aaaccgctaa
     8821 gcctcttttc tcccgaagtt cttaatatca tgtcaggtga tgcttgtggt aagtgtgacg
     8881 tatagaggga tgattctatg acttctttat ctattgaatc cacatctaga gaaccatcct
     8941 ttattctatg agcaatcttt ttcacagcgt caactaactc gtgctgacct ccataagcta
     9001 tggctatgtt cagataatga ttatcataat ttattgtagc ttgatctaat ctgttcaaaa
     9061 catcctttag tgattctggt aagagttcaa cacgaccaat ggcttttact ctcatcctgt
     9121 ttctgtgtat tctgggatcc ttgtacagct tttctaatct ggttttaatc aaatcatata
     9181 gataatgtaa ttcatcatca tcgcgagata ggttctctgc agataaaaca tacaaagtga
     9241 tgatctttat tcctaattct tcacaccaat ccagcaggtt ttctactgca tcagctcctc
     9301 gaaagtgccc atcttctttt acaataagat ttctttttgc ccatctgcga tttccatcta
     9361 gtattatcgc aatatggttt ggaaggttac cgtttctgat ttctttttca agtttgtttg
     9421 aataaatctt gtataatccg gagaaattca ttacactatc ttttattccg ataacacttc
     9481 accgcccttc ttctttctat atttgcggaa gaggattgtg gcggctatga gtattactgc
     9541 taatcctaat aataatggag gaagaagagt aaacgggcta tcatcatgtg tcattatgtt
     9601 tgtaaactgt aataccactg ggtacaaagt tactagaact attattctga gaatgtctgt
     9661 aactgatcta atgcttccct tgaaccaact gcttagtaat gttcctccaa acacgcatcc
     9721 catgcccatc cataggaatg gaagtgctcc agaaacatag agtcctatta tcaccttgtt
     9781 tgttactatg cttactatgt cgggttgagc ttgaataagc gatggattga cggcactaat
     9841 tccagacggt atggacacta acatcaaaag agctccagct accattgtga aaattctaat
     9901 gaatccagct ggcgtaggtt ttgtccaatg cgaccaagct ctgtctacat caaaagctct
     9961 gataaggaat gctccgccaa gtatgcttac caatactgca aaaatttctt tagttattcc
    10021 caacacagat gcaactcctc cgatcaaaag taaaattcct ggaactccaa ggaaaaattt
    10081 ggaatacttt gaatcaaatg caattgctcg caaatatctt ccaagaacag cgtaagaata
    10141 ttctacgctc ctgcttactc gcataaccac tctgcgaaca gaaacaattg gtaaaacatt
    10201 ttgaattatt ggaattacac tttcgtcgtc ttctccgtct gaaacaatta ccgcacctgt
    10261 tgctctatat tgttcaacaa ttgttttgac ttggccaact attttttcat cagcttgcac
    10321 tcctctactt tctgttccag ctaccactat tacttctgct tggtatcctt tacttacaag
    10381 atcctcatac gttttaactg cagcaaaaat cgaattggaa tctgcatctt ccggatcttc
    10441 taaagctaat ctttgtgcag cttcaatgca tgcatttctg ccaatgactg gtgttgtaac
    10501 tccggctttt ttaccaatat catcatctct atcaacacaa attactagta gcttgctagt
    10561 cttggtttca gtcacttttt tttctaaatt ctcattgact gacattatct ctaccaaaaa
    10621 ttacatttcc ccattaatta atttacatta ctactatact gaaccgatta ttatcaaaag
    10681 atttgacatg agatttctgg tcttatggcc ttgtctcatc tgatttgaga gaaaaagact
    10741 cgaattcttt ctttaaagca tctagtgtct caaggttccc ctgtatgtca tttgcacctc
    10801 tttccttgat tatgtttgcc aaaactattg tctctctcaa gtaactgttg tagcttttca
    10861 gagattcacc gtaatttagg tagctctcat gccactcttg agaggctcca ctttgaatca
    10921 attctattat gagtgaattg atctgtgaac tggatgcttc agctctcatg atgaaattgt
    10981 ctggtgagac tgtaccggca agcatagctt gcaagtcatt ttgtatctca tctgttataa
    11041 caaaatgtct tgcctttaca ctctcaagat gatctctata gtcagaaaca acgaaactcg
    11101 aatcgtcgcc ttgtggtatg agccatactg aaaagcttgc agctgtaatt gcggccaata
    11161 ttgcacctgt gattatgatg cttctttttt ttgtcaactc actgaatctc tccaagacgg
    11221 ttaaaagtat aactaaaaac cactcaaatt tacatttaca tgtgcacaaa tttgctaatt
    11281 tcagctagtt ggatcattta ctcattagaa atatcaataa attgtaattt tttatagaaa
    11341 ttatgtacgt actaggcagg aagtaaaatg taaaatttaa ccgcctctag cgtatagtga
    11401 aataattttt acatctcctt cctaaataca acttccttcg taaaactgtg aatgacacaa
    11461 gcatactgtg taaaatgcag gaagaaagtc gatatttcaa acccaaaaga agttaagcta
    11521 aagaacggtc gacctgcagt aaagggcaca tgtccaaagt gtggcactaa cgtcttccga
    11581 ataggaaagc cttagaggac cactttcttc ttttttaatt aaatccatat agggcacacc
    11641 taagaaatta atttgtggca aaatcggaat acgagaaact cttaaagaaa attcaagaca
    11701 agatttcaga aaataaaaaa gattctgttt ccagatttga acttccaatt gtagatataa
    11761 tgtggcaagg caacagaaca tttttccgca atttcgcaga atttccaaag atcctaagaa
    11821 gggacccaga taaagtacta caatacctgt ctaaggaatt tgcttctcct gcacaaactg
    11881 ccggtgacaa ggctatcttt gtgggaaaaa aggatccgca tgattttaca gcactttttg
    11941 ccagatacgt aaaagaatac ctagagtgcc ctacgtgcaa aagccctgat actagagtgg
    12001 agaaatcaaa cagaatgacc tttcttattt gtgaagcatg cggagctaaa tcgtcgctaa
    12061 aaggccaata tgcgtaatgt cgtttgcagt acgaacagtt gcctaccaac agcatctgat
    12121 gctcaacata tgtgacgcga atctcgttgg gaaaacaata accaaatctg atttttcatt
    12181 aaacataagt agtagctatt ttgcagaacg ctttgtagaa aaaacagaag cagaagaact
    12241 tctcaaaaaa tattccataa taaacatggt tggaaaggaa acaattgctt tagcaataga
    12301 actcagaatt ggttcatcaa aaggagtaaa agaaattgag ggtgttccgt ttcttttagt
    12361 tttcaaattc tgataaaaca gcacaattat atgtattcaa gcagaattat tttttgatcg
    12421 ttgtgggaaa aagaaaagtt ctaaacgaaa gcgaactaaa agaaatgaaa cttccagaag
    12481 aaggagaact tcttggcaga gttatcaaac ttttaggtag tgatcatgtt ttggtaaaat
    12541 gtactgatga taaaacaaga atgggaagaa tacgaggaaa actaaaacga aaaatatgga
    12601 ttagagataa tgatgtagta acacttgcac catgggattt caggactgag gacagaggag
    12661 acattacatg gagatacacg ttatcacaag ttgattggct caaagacaat ggccacctac
    12721 caaaagattt ctgaatacgc aatcgatttt acttagtaaa tttaattcat aatattgtca
    12781 gatgaacttg gaaaaaaatt agcaaatcgc attgatcgtg aggttcttgc aaatgaaagg
    12841 cgaggaagat cagaaaaagg tgtccttgat aaaatcaagg tcatggatga tgtcctagac
    12901 aaaactacca taatgactct gtccaagttg ataaattcag gtgtcatatc atacgttaat
    12961 ggagtagttg gctctggcaa ggaatccaag atgtattggg ctgtggatcc tgatggaaag
    13021 gaccttgctc tcaagatata tctggtaaca gctgcaaatt tcaaaaaaag gcttccatat
    13081 cttgtggatg acccacgatt ctctcatatc aaaaagggta cgagaaactt ggtggagctc
    13141 tgggcacaaa aggaatttcg aaatctgaat cattgtatta aaagtggtat tcctgtaatc
    13201 aaaccaatcc acgttataaa aaatatcttg gtactggaat ttgtgggaaa aaatggtgcc
    13261 cccgcaaaaa ctcttgtaga gacagaaatt gatgaaaatg actataaaga tgcaatttca
    13321 atcatttcac aactctacaa aaaagctaaa cttgtacatg ctgacttttc agaatataac
    13381 atctttaaaa cagaaaatgg actgattctt ttcgatctag gatcggcagt tgatgtaaga
    13441 cacaaaaatg caaaagaatt ccttgaaaga gatattaaga atataactag attctttgtt
    13501 aaaagaggct tgactgtgga aaatccatct gacatatttg cgaggatagt aaattgagct
    13561 ttgaaaaatc tgttcttatc ccacttgata ggattggcgc acttattggt aaatctggaa
    13621 aagtaaaatc tagaattgag aagacatgtt ctgtcaaaat agtagtaaat agtgaaactg
    13681 gtgagataat ggttagtggt gtaggaagca tagaagatat gttgcccttt aaggcagtag
    13741 aaattgtaat gacaataggc cgaggattct ctcctgagaa agcaatgcgt ctcttgcaag
    13801 gagaaaatgc tttacacgtg atcgatttga gagaatttgc tggtagatca tcttctaatg
    13861 ttgaaaggat aaaaggcaga ataataggag aagggggtag agtgagacaa aatatagaag
    13921 agcttagcgg tgctaatatc tcggtttatg ggcgtactgt atcaattatc ggggagggaa
    13981 atcaactacg taatgccgtt gatgcgatct ctgccctttc aagcggtagc atgcatggtg
    14041 cagtttacaa tgaactgcaa gaagcaagac gcagggaaaa attagacaga ctacgactgt
    14101 gggaaaatag caatgtcttc gactaaggaa atttttagtc agatctcacc cagtgagttc
    14161 ttttacagaa accgagactt ggcaggtttt agtaatccta caagatcact ttacacatct
    14221 gttagagaat ttgtagaaaa cgcgttggat gcatgcgacc aaaaaggaat actgccagac
    14281 ataacactga caataaaagc agtagatcca gagcaagcag atccaaaacc atacatactt
    14341 tctgtcaaag acaatggtcc tggtattgag ccaaaacatg tgccgctggc atttggaaca
    14401 gttctttatg gttcaaagtt tggactaaaa caagctagag gaatgttcgg gcttggtgca
    14461 acaatggcaa tactttatgg tcaaataact acaaacaaac ctgttacagt aaaaagctgt
    14521 gcagatggaa aaacacaaga tgaatttgta atgctgcttg atatccaaaa aaacaaaccc
    14581 atcatacaaa agcacgttac aaaagaagct tcaaagacag gcctctcagt aagcattgtt
    14641 cttgagggcg actattcaaa ggcaggttcc aaaattcgtg attatgtctt acaaacatca
    14701 ttgatcaccc cctatgcctc aataacattt gaagaaccta ctggtgaacc acacaaatat
    14761 ccaagaattg tcaaagagat gcctcctcct ccaacaatca ttaaacctca tccacatgga
    14821 atcgatgtcg agacaattag aagaatgatg gtcgacacac actaccagat tccagtcgtt
    14881 gacaacaaaa tgattacaaa agtaagaagt gaactaggat tacaaagaaa aaatctaaac
    14941 gcaaaaggca tattggaaag agcagaaaaa cggtggtcta atctttccag accagttaga
    15001 atagtcatag caatgatgtc ttttcttaac ct
//
LOCUS       DATIDL010000146         4499 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrosopumilaceae archaeon isolate SMAG_U16342
            k141_988255, whole genome shotgun sequence.
ACCESSION   DATIDL010000146 DATIDL010000000
VERSION     DATIDL010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935836
            Sequence Read Archive: SRR25158326
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosopumilaceae archaeon (soil metagenome)
  ORGANISM  Nitrosopumilaceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrosopumilales;
            Nitrosopumilaceae.
REFERENCE   1  (bases 1 to 4499)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4499)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/04/2024 05:09:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,520
            CDSs (total)                 :: 1,474
            Genes (coding)               :: 1,474
            CDSs (with protein)          :: 1,474
            Genes (RNA)                  :: 46
            rRNAs                        :: 1 (5S)
            complete rRNAs               :: 1 (5S)
            tRNAs                        :: 43
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4499
                     /organism="Nitrosopumilaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_988255"
                     /isolate="SMAG_U16342"
                     /isolation_source="soil"
                     /db_xref="taxon:2202732"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..329
                     /locus_tag="VLB45_06615"
     CDS             <1..329
                     /locus_tag="VLB45_06615"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HSD05404.1"
                     /translation="SPVMKNGKRFKIYRGMASFYASLGRKSKETGTISISEDLNDYVA
                     EGVEAMVPYKGSVTDIIKQLTGGIRSGLSYCGADTITQMQENAEFMKMSRAGFVESQP
                     HDVDLM"
     gene            365..829
                     /locus_tag="VLB45_06620"
     CDS             365..829
                     /locus_tag="VLB45_06620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05405.1"
                     /translation="MLSKRTAIGIGAGSVITALGVISLILAFGVQTQTIDESVDIGKN
                     TIYKFNAQEHFHEILNVTGTSFHVQLKTPSTGLQVDQDFKNEVSFEWYSLEDGEHRIE
                     VTNTGDSQLHVFGTLQFTSDPILFTYHMLVIISGVIIIGISAGFTIRKPRGF"
     gene            complement(826..1641)
                     /gene="pheA"
                     /locus_tag="VLB45_06625"
     CDS             complement(826..1641)
                     /gene="pheA"
                     /locus_tag="VLB45_06625"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HSD05406.1"
                     /translation="MQKIAFQGERGAYSEAAAFYFFKEGIEAIPYPTFYDVLEAAEND
                     KVSYAVLPIENSLEGSVGESYDLLLSTTLNTVGEIYFRIKHCLIGFQSLEKINTVYSH
                     PQALGQCRKFIQNHKMKTVPTYDTAGSVKILLELKDDNSACIASKRAAEIYKVPVIKE
                     GIEDNANNYTRFLVLAKNKHEKTGKDKTSIIFSIKHVPGALYSILEKFNTNKINLTKI
                     ESRPTKSTPWEYNFYADFEGHESESNVKDVLEKIKHHTTFLKILGSYPRAEIS"
     gene            complement(1647..2201)
                     /locus_tag="VLB45_06630"
     CDS             complement(1647..2201)
                     /locus_tag="VLB45_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosome complex RNA-binding protein Csl4"
                     /protein_id="HSD05407.1"
                     /translation="MSEKFSLPGDKIATIEEFETGDNTFDDGHTIRSVVVGSREFDKT
                     NRIAKINRLKSPAVPQVNDLVIGNVAALMNNMFAVTMLYINGKPTHSGLECICQAKGA
                     KKRIITRVSDIVMVKVISHLNGAIHATISEPDLGVLFTQCNKCAGKVVPLGGNVKCVD
                     CGYIEERKLSSKFGNGDFIKLGSK"
     gene            complement(2234..3229)
                     /gene="dph2"
                     /locus_tag="VLB45_06635"
     CDS             complement(2234..3229)
                     /gene="dph2"
                     /locus_tag="VLB45_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043364 - glycyl-radical enzyme activating
                     activity [Evidence IEA];
                     GO_process: GO:0017183 - protein histidyl modification to
                     diphthamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphthamide biosynthesis enzyme Dph2"
                     /protein_id="HSD05408.1"
                     /translation="MIVIDEKRIFSEIQNRKPVSVALNGPDGILPKIQETASKIMEEF
                     GIPAYVLADTCFGTCDMNTNGAKVLGAEILFHIGHTINDTSFGENVVLVDAFDDISFD
                     SVTRKCAAELAGKTVSLVTDSQHLPQMERVKKILEEGGVIVRIGEGKGQLNDGQVFGC
                     EFYPTSETKDIVDANVFLGQSAFHASGIALSTGKPTYLLDPYFDEMREVTEFAQKLQK
                     KSILAVYKAAEAETFGVIVGLKEGQFSKTTALKFKKELEKAGKKVQLFALTDITDDKL
                     GNLKGIDAFIQVACPRISTDNHFHKPVLSTPQANALLKLLKNESIDGFLQIPHWL"
     gene            3314..3550
                     /locus_tag="VLB45_06640"
     CDS             3314..3550
                     /locus_tag="VLB45_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020265886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HSD05409.1"
                     /translation="MAIAFVLINAELGAENELVNQLKAIEHVTYVYVLYGAYDLVVKI
                     EAPDSATLKKTISNNIRQLKNVRSTLTMTVIDEN"
     gene            3540..>4499
                     /locus_tag="VLB45_06645"
     CDS             3540..>4499
                     /locus_tag="VLB45_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007551416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(Ile)(2)-agmatinylcytidine synthase"
                     /protein_id="HSD05410.1"
                     /translation="MKTSSVIHIGFDDTDSRKGMCTTFLAYKIVEYLKKENVKFLDYP
                     YLIRFNPNIPWKTRGNGAVAIKIETDKPDTVKKNIIRFIKKYSSTNDGANPGLVFYQQ
                     EKIPKCFSEFGQIALCKLINRNKAKEFASKNGLEAFYLGNGQGLVGAIGAIGYFFDDH
                     TFELISYRNESNFGTKREIFKESVKAMQNMTSPKTFNSYDEEKNRVLIAPHGPDPVLF
                     GVRGEDVNSVIQGASLVKSAEKFDGYMVFRSNQGTGAHLKNELDVNNLKPFSSGTITG
                     KVSISPKIELGGHVVFSIVKNGTQVQCAVYKPTGMTKIASKLIT"
BASE COUNT         1376 a          862 c          825 g         1436 t
ORIGIN      
        1 gctcacctgt aatgaaaaat gggaagagat tcaaaatcta cagaggcatg gcctcctttt
       61 atgcatcact tggaagaaaa tccaaagaaa caggaaccat atcaataagc gaggatctca
      121 atgattatgt agcagaagga gtagaggcga tggttccgta caagggaagt gtaactgata
      181 tcataaaaca actcaccgga ggaatcagat ccggattaag ctattgtggt gcagatacga
      241 ttacccaaat gcaagagaat gcagaattta tgaaaatgtc aagagcaggt tttgtggaaa
      301 gtcaaccaca cgacgtagat ctgatgtagt tcacttttaa tatagtatag aaattattcc
      361 ttgtatgctc agcaaaagaa ctgcaattgg aataggagca ggcagtgtca taactgcact
      421 tggtgtgatc agcttaattc ttgcatttgg agttcaaact cagacaattg atgaatcagt
      481 tgacatagga aaaaacacca tttacaagtt taatgcacaa gaacactttc atgagattct
      541 taatgtgact ggcacatcat ttcatgtaca actgaaaacc ccatctaccg gactgcaagt
      601 agatcaggat ttcaaaaatg aagtaagctt tgagtggtat agcttggaag atggtgaaca
      661 tagaatagaa gtaaccaata ccggcgattc acaacttcat gtatttggca ctttacaatt
      721 tactagtgac ccaattcttt ttacatatca catgcttgta ataatttcag gcgtcatcat
      781 tattggtatc agtgctggtt ttacaataag aaagcccagg ggcttttaac tgatctctgc
      841 tcgtggatac gagccaagta tttttagaaa agtggtatga tgctttattt tttcaagcac
      901 atctttcaca ttagattcgc tttcatggcc ttcaaaatct gcatagaaat tgtattccca
      961 cggcgtactt ttagttggtc ttgattctat ctttgtgaga tttattttat ttgtattaaa
     1021 cttctcaagt atgctgtata atgcaccggg aacatgcttt atagaaaata ttatggaggt
     1081 cttgtctttt ccagtctttt catgtttgtt ctttgcaagc actagaaacc gagtatagtt
     1141 atttgcatta tcctctatgc cttcttttat cacgggaact ttataaattt cggcagcacg
     1201 tttacttgca atacaagctg aattatcgtc ctttaattca agcaaaattt tcacacttcc
     1261 agcagtatca tacgttggaa ctgttttcat tttgtggttc tggataaatt ttctgcattg
     1321 gccaagtgcc tgtggatgtg aatatacagt attgattttt tctagagatt gaaatcctat
     1381 aagacaatgc tttattctaa aataaatttc gcctacagta ttaagtgtgg ttgataaaag
     1441 cagatcatag ctttcaccca cactgccctc tagggaattt tctattggaa gtacagcata
     1501 gcttaccttg tcgttttctg cagcctccaa tacatcataa aatgtagggt atggaattgc
     1561 ctctatgcct tctttgaaaa aataaaaagc agctgcttca ctgtacgctc ctcgctctcc
     1621 ttgaaatgca attttttgca ttgttcttac ttggatccta gttttataaa atcgccgttt
     1681 ccaaacttgc ttgaaagctt tctttcttca atatagccac aatctacaca ttttacattg
     1741 ccaccaagag gtacaacttt tcctgcacat ttgttgcatt gggtaaacaa aacaccaaga
     1801 tctggttcac taattgttgc atgtattgcc ccatttagat gactgatcac cttcaccatg
     1861 actatgtcgc tgactcgcgt aatgattctc ttttttgcac cttttgcttg acatatgcat
     1921 tcaagtccag aatgtgtcgg tttgccgttt atgtaaagca tggttactgc aaacatgtta
     1981 ttcatcaatg cagcaacatt acctatgact agatcattta cttgtggaac tgctggtgat
     2041 ttgagcctgt taatttttgc aatgcgattg gttttatcga attctcttga accaactaca
     2101 actgatctta tggtatgacc atcatcaaat gtattgtcgc cagtttcgaa ttcctcaatt
     2161 gttgcaattt tatccccggg taaactaaat ttttctgaca tgtgtgaatt ttgaagttgt
     2221 taattataat tgtttatagc caatgtggta tctgcaaaaa tccatcaata ctctcatttt
     2281 ttaatagttt taaaagcgcg tttgcctgcg gtgtagaaag cactggtttg tgaaagtggt
     2341 tgtctgtaga tattctaggg catgccacct gaataaaggc gtcaattcct tttagatttc
     2401 caagtttatc atcagtaata tcagtgagtg cgaagagctg aactttttta cctgcctttt
     2461 caagctcttt tttgaatttt aatgccgtag tttttgagaa ctgtccctct ttgagaccga
     2521 ctatgactcc aaatgtttct gcttcagctg ccttgtatac tgcaagaatg gattttttct
     2581 gcaacttttg ggcaaactct gtaacttccc tcatctcatc aaaatatgga tcaagtaggt
     2641 atgttggctt gccagtggag agtgcaattc ccgaagcatg aaaagcgctt tggcccaaaa
     2701 acacatttgc atctacgatg tcttttgttt ctgatgttgg ataaaactcg caaccaaaga
     2761 cctgaccgtc atttagctga ccctttcctt ctccaattct aactatgaca ccaccttctt
     2821 ctagaatttt ttttactctt tccatctgtg gaagatgttg gctgtctgtt acaagtgaaa
     2881 cagttttccc tgcaagctct gcagcacatt ttcttgtaac actgtcaaac gaaatgtcat
     2941 caaacgcatc cacaagaaca acattttctc caaatgatgt gtcgtttatg gtgtgtccta
     3001 tgtgaaaaag tatctctgct cctagcactt tggcaccgtt tgtattcatg tcgcatgtac
     3061 caaagcacgt atctgcaagg acatatgcag gaatgccaaa ctcttccatg atctttgatg
     3121 cagtttcttg aatttttggc aatatgccgt caggtccatt caaagctaca gaaacaggtt
     3181 ttctattttg gatctcagag aaaattcttt tctcgtctat tactatcatt tatgttctaa
     3241 aactcattaa tttttaattt aaatcaaccg aagctacata atatttaata tcgcatgttc
     3301 taacaccaaa ggcatggcaa tcgcatttgt tctcataaat gcagaacttg gagcagaaaa
     3361 cgaactagta aaccaactaa aagcaattga acatgtcaca tatgtctatg ttctatatgg
     3421 tgcctatgac ttggttgtaa aaatcgaggc tcctgacagt gcgactctca aaaaaaccat
     3481 ctcaaataac ataaggcaac tcaaaaatgt ccgttctaca cttaccatga ctgtaataga
     3541 tgaaaactag ctctgttatt cacataggct ttgatgatac tgactcaaga aagggcatgt
     3601 gtaccacatt tcttgcctac aagattgtag aatatctaaa aaaagaaaat gtcaagtttt
     3661 tggactatcc atatctgata agatttaatc ccaacatacc gtggaaaacg cgcggaaatg
     3721 gagcagtggc aataaaaatt gaaactgata aacctgatac tgttaaaaaa aacataatcc
     3781 gattcatcaa aaaatattct tcaacaaacg atggtgcaaa ccccggtctg gtattttatc
     3841 agcaagaaaa aatcccaaag tgcttttctg aattcggaca gattgcactg tgcaaactca
     3901 taaacagaaa caaagcaaaa gaatttgctt caaagaatgg attggaagca ttctatcttg
     3961 gtaatggaca gggtttggta ggtgctatcg gcgcgatagg ctatttcttt gatgatcaca
     4021 cttttgagct gattagctac cgaaatgaat ctaactttgg tacaaaaaga gagatcttca
     4081 aagagagtgt taaagcaatg cagaatatga catctccgaa gacgtttaac agctatgatg
     4141 aagaaaaaaa cagagttctg attgcaccgc acggcccaga tccggtactt tttggtgtac
     4201 gaggagaaga tgttaattcc gttatacaag gcgcgtctct tgtgaaatct gcagaaaagt
     4261 ttgatggcta tatggtattt agatcaaatc aaggtactgg cgctcacttg aaaaatgaac
     4321 ttgacgtaaa taatctgaaa ccattctcat caggaactat tacgggaaag gtgtcaatat
     4381 cacctaaaat tgaactaggt ggtcatgtag tattttctat tgttaaaaat ggcactcaag
     4441 ttcagtgtgc tgtatacaaa ccaacaggca tgactaaaat cgcatcaaaa ctaatcaca
//
LOCUS       DATIDL010000147         3620 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrosopumilaceae archaeon isolate SMAG_U16342
            k141_1392080, whole genome shotgun sequence.
ACCESSION   DATIDL010000147 DATIDL010000000
VERSION     DATIDL010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935836
            Sequence Read Archive: SRR25158326
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosopumilaceae archaeon (soil metagenome)
  ORGANISM  Nitrosopumilaceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrosopumilales;
            Nitrosopumilaceae.
REFERENCE   1  (bases 1 to 3620)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3620)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/04/2024 05:09:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,520
            CDSs (total)                 :: 1,474
            Genes (coding)               :: 1,474
            CDSs (with protein)          :: 1,474
            Genes (RNA)                  :: 46
            rRNAs                        :: 1 (5S)
            complete rRNAs               :: 1 (5S)
            tRNAs                        :: 43
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3620
                     /organism="Nitrosopumilaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1392080"
                     /isolate="SMAG_U16342"
                     /isolation_source="soil"
                     /db_xref="taxon:2202732"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..852)
                     /locus_tag="VLB45_06650"
     CDS             complement(<1..852)
                     /locus_tag="VLB45_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HSD05411.1"
                     /translation="MAKGQDLSVSLEEFGLSQYEAQAYVTLITKGTISAGELAYYSNL
                     PRTKVYPTLLKLVKKKLAIISKSKPIMCTGIAPEDAFDEIVQEQINRVNGMNSLVTKL
                     KQVSEDSKKARGSEEKRYFHLTPNYVFKQLETMIDSSKTSVHAMVDSWGLNLFSQCKT
                     TLIHALRKNIDIKIIIPPSLVCTEAFQTIPDDVKIKTSDISHNSITFDDSEILMINSN
                     NGKGAIFLSTEVLGTNQAKLFEQTWKNATKINNLSSMTKTEAQETLKIIEMINENGLG
                     FVLDTLLH"
     gene            1012..1575
                     /locus_tag="VLB45_06655"
     CDS             1012..1575
                     /locus_tag="VLB45_06655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05412.1"
                     /translation="MVIQSKWVISDETTNQVTRAITIKPRLEYAASLVDGILDILAQK
                     RNDLDKSNLPLYVQMNETKSSSEEFQNKMHLEIEISRAIEALRQAHRCLDSVFGLGNV
                     PLVVSPTISIVRTVRSKLFGLIPEVDANLGELSLVLGGLIIDTGHLTSATLDFEAANL
                     KSARLLDEAKLIADSKICKQFPNLDFP"
     gene            1588..2106
                     /locus_tag="VLB45_06660"
     CDS             1588..2106
                     /locus_tag="VLB45_06660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05413.1"
                     /translation="MLNFTKNASSEDIPNHLYQIHMLSSKIEQKIAQKSSDKDSPFIE
                     SLSVEELKDLRQVMMAADYLLSKYQDKKDLKEFLAEFIGIIMHAANSVNILRDDLEEL
                     VLSAETALQQIQNLHEDVTQNLALGRFVQTKIDDYKEEHKVVPKPSPINLTNPSTPVY
                     TQAYLTKTAVEI"
     gene            2119..3306
                     /locus_tag="VLB45_06665"
     CDS             2119..3306
                     /locus_tag="VLB45_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007550904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HSD05414.1"
                     /translation="MSLAPQELENSASKFAAEAIKLDSQGARGMAISNYQRAIESLVK
                     LVQLYPDNKLNKVYMERSAAYQNRIKALQLTNVEIEPAVDPKATPEQQKQQLAKNDFD
                     ELVMKEKPNVSWAEVIGLDDAKDALRESIVYPSKRPDLFPLGWPRGMLLYGPPGCGKT
                     ILAAATASEIDGYFINVDAASMMSKWLGEAEKNVSKLFTMARGYAEKEGLPVILFVDE
                     VDSLLGNRNSEVGGEIRVKNQFLTEMDGINGKGKDLKLYVIGATNKPWSLDWPFLRRF
                     TKRIYVPLPTLDARTNLFELYSSPLKRDEKVKPVELAKLADGYSASDIRDICQSAQMM
                     VVNELFNSPNYSSETVVGEPRQQPRDLTMSDFKEILTRRKPSVSMEMIRAYYKWSEQF
                     KAL"
     gene            3421..>3620
                     /locus_tag="VLB45_06670"
     CDS             3421..>3620
                     /locus_tag="VLB45_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase domain-containing
                     protein"
                     /protein_id="HSD05415.1"
                     /translation="MPTKKAKHANKFGKLVLEDGTVFDGMGFGYSTTVFGEAVFNTGM
                     VGYVEALTDPSYSGQILALTYPL"
BASE COUNT         1175 a          671 c          702 g         1072 t
ORIGIN      
        1 atgaagcaag gtgtctagta caaacccaag tccgttctcg tttatcatct cgattatttt
       61 caaggtttct tgtgcttcag tctttgtcat ggaagaaagg ttgtttattt ttgtagcatt
      121 tttccatgtc tgctcaaaga gttttgcctg atttgtaccc aaaacttcgg ttgacaaaaa
      181 tatggcgcct tttccattgt tgctatttat cataagtatc tcagaatcgt caaacgttat
      241 agagttgtga gaaatgtctg atgtttttat ttttacatca tctggaattg tttgaaaagc
      301 ttccgtacac acaagactcg gtggaattat tattttgatg tcgatgtttt ttcttagtgc
      361 atgaataagt gtggttttgc attgtgaaaa aagattcaat ccccatgaat caaccattgc
      421 atgaaccgaa gttttggaac tatcaatcat ggtttcaagt tgcttaaaga catagtttgg
      481 tgtaaggtga aagtatcgtt tctcttctga gccccgggct ttcttgctat cttcgcttac
      541 ttgctttagt ttggttacaa ggctgttcat tccgtttact ctgttgatct gctcttgtac
      601 tatttcatca aaagcatctt ctggtgctat gccagtacac attattggtt tgctttttga
      661 tatgattgca agttttttct ttaccaattt tagtaaagtc ggataaacct ttgttcttgg
      721 aagattggaa tagtatgcta gctcgcctgc ggaaattgtt ccttttgtaa ttagtgtaac
      781 atatgcttgt gcttcatatt gacttaaccc aaattcctct agactgactg aaagatcctg
      841 tccctttgcc attgccaacc ttttgatagt tataaggtaa gtccaatagc taaattcatt
      901 gaacaaacct ttgaaaaaat actaagctgt atctgcagat tgcgtcacta tggtaacaca
      961 aatccgtgta acacgggtct gatatagaaa aatccatctt ctagtttggg catggtaata
     1021 caatctaaat gggtaataag tgacgagaca acaaatcagg taacgcgcgc aatcacaata
     1081 aaaccaaggc ttgagtatgc cgcaagtctt gttgatggca tacttgacat tcttgcacaa
     1141 aaaagaaatg atcttgacaa aagcaacctt cctctttatg tacaaatgaa tgaaacaaaa
     1201 tcttcttcag aagaatttca aaacaaaatg cacttggaga tagaaatttc tcgtgccatc
     1261 gaggcactaa gacaagctca caggtgtctt gattctgttt ttggattggg taacgtgcct
     1321 cttgtagtgt cgcctacaat ctcaattgtg agaacagttc gttcaaaact ctttggtctt
     1381 attccagaag tagatgccaa tttgggtgaa ttgtctttgg tgcttggagg ccttattata
     1441 gacaccggtc atcttacaag tgcaacactt gactttgaag cggcaaatct aaagtctgct
     1501 cgacttttag atgaagcgaa gttgattgct gactctaaaa tttgcaagca gtttcctaat
     1561 ctagatttcc cgtaagcaag agccatcatg ttaaatttta caaaaaatgc gtcttctgag
     1621 gatatcccaa atcatttgta ccaaatacac atgctttcct ctaaaataga acaaaaaatt
     1681 gcacaaaaat catcagataa agattctcca ttcatagaaa gcctttcagt tgaagaacta
     1741 aaggatctca gacaagtcat gatggcagct gattaccttc tatctaagta tcaagataaa
     1801 aaggacctta aggaattttt ggcagaattc ataggaatta taatgcatgc agcaaactct
     1861 gtcaacatac tacgggatga tcttgaggaa cttgtccttt ctgcagagac tgccctgcag
     1921 cagattcaaa atctgcatga ggatgtaacg caaaaccttg ccttgggaag gtttgttcaa
     1981 acaaagatag atgactataa agaagaacat aaagtcgttc ccaaacctag tccaattaat
     2041 ttaaccaatc cttctacacc agtttacacc caagcatacc taacaaaaac cgcagtggaa
     2101 atttgatagt gagaataatt gagtctagcc ccgcaagaat tagaaaatag tgccagtaaa
     2161 tttgccgcag aagcaattaa gcttgattct caaggtgcgc gaggcatggc aatatcaaat
     2221 tatcagagag ccattgaatc tctcgtaaaa ctagtacagc tgtatcctga taacaaacta
     2281 aacaaggtct acatggagcg ttctgccgca tatcaaaaca ggataaaggc attgcaacta
     2341 actaatgttg agatagaacc tgccgtagat ccaaaagcaa ctcctgagca acaaaaacaa
     2401 cagcttgcaa aaaatgattt tgatgaattg gtaatgaaag aaaagcccaa cgttagctgg
     2461 gctgaagtaa ttggacttga tgatgcaaag gatgcattac gagaatcaat agtatatcca
     2521 agcaagagac cagatctttt cccacttggc tggccaagag gaatgctgct ttatggtcct
     2581 cctggatgtg gaaagacaat tcttgctgca gcaactgcaa gcgagattga tggttatttc
     2641 ataaatgttg atgcagcttc gatgatgagc aaatggcttg gtgaagcaga aaaaaacgta
     2701 tcaaaattat ttacaatggc tagaggctat gctgaaaagg aagggctacc agtaatattg
     2761 tttgtagatg aagttgattc acttttagga aatagaaata gtgaagttgg cggagaaatc
     2821 agagtaaaga atcaatttct tacagaaatg gatggaatta acggtaaagg aaaagatttg
     2881 aagttatatg taattggtgc aacaaacaaa ccttggagtc ttgattggcc atttttgaga
     2941 agattcacaa aaagaatcta tgtcccacta cctactcttg atgcaagaac aaacctgttt
     3001 gaactttaca gctctcctct aaaaagagat gaaaaagtaa aaccagtaga acttgcaaag
     3061 cttgcagatg gatatagcgc aagtgacatc agagacattt gccagtctgc acaaatgatg
     3121 gttgtaaatg aattattcaa ttccccaaac tattcatccg aaacagttgt tggagaacca
     3181 agacaacaac ccagagacct taccatgtct gactttaagg aaatactcac tagaagaaaa
     3241 ccaagtgtca gtatggaaat gattcgcgcc tactataaat ggagcgaaca attcaaggca
     3301 ctatagaaaa gccgatcgct aaaattttgt aatatgattt tgtaacctct aatatcttag
     3361 cggatctatc tatcaaaaaa cttaaatctc caatagaatt cacttcgtga gggttcaaaa
     3421 attccaacaa aaaaagccaa acacgcaaac aagtttggca agcttgtcct tgaggacgga
     3481 accgtttttg atgggatggg ttttggttat tctaccactg tttttggaga agctgtattc
     3541 aacactggaa tggtaggata tgtcgaagca ttaacagatc cttcctatag tggccaaata
     3601 cttgcactca cataccctct
//
LOCUS       DATIDL010000148         5477 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrosopumilaceae archaeon isolate SMAG_U16342
            k141_2000594, whole genome shotgun sequence.
ACCESSION   DATIDL010000148 DATIDL010000000
VERSION     DATIDL010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935836
            Sequence Read Archive: SRR25158326
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosopumilaceae archaeon (soil metagenome)
  ORGANISM  Nitrosopumilaceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrosopumilales;
            Nitrosopumilaceae.
REFERENCE   1  (bases 1 to 5477)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5477)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/04/2024 05:09:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,520
            CDSs (total)                 :: 1,474
            Genes (coding)               :: 1,474
            CDSs (with protein)          :: 1,474
            Genes (RNA)                  :: 46
            rRNAs                        :: 1 (5S)
            complete rRNAs               :: 1 (5S)
            tRNAs                        :: 43
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5477
                     /organism="Nitrosopumilaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2000594"
                     /isolate="SMAG_U16342"
                     /isolation_source="soil"
                     /db_xref="taxon:2202732"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(283..903)
                     /locus_tag="VLB45_06675"
     CDS             complement(283..903)
                     /locus_tag="VLB45_06675"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HSD05416.1"
                     /translation="MSLIIFLRHAQAKNNVERILAGRTKGFPLTDLGLQQANNAASFL
                     GALKISAIYSSPIERALNTAQIVSDKLNLNLKVDDRLTEIEMGNFSGMAYEDMFTKHG
                     NVFLKFYQDNPVIAQNGVETFSSVKKRVLDMVNYCSKQHKNQNVLLVTHMDPIKAMLS
                     TILQLKPESLYELIIQNASLNIIKKEQNTFSFVAINSMNADRYSQV"
     gene            959..1630
                     /gene="npdG"
                     /locus_tag="VLB45_06680"
     CDS             959..1630
                     /gene="npdG"
                     /locus_tag="VLB45_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent F420 reductase"
                     /protein_id="HSD05417.1"
                     /translation="MKIGIIGGTGGMGKGFALRWCVNHDILIGSRDAVRATEAAIEYS
                     KNAAEFHQNMKGKITGNDNISVAKESDVLVLSIPYENIESTCATLLSNIKNTCTVVSP
                     IVPMTKTDAGFEFISFIEKKPSAFEMVQKHMKNKTKLVSAFHTVSEKKLTDPNLILDF
                     DIFVCGDDQDSINIVNGLIKEIKNLRPIFLGPGSLSYLAEVATPILLNVMIKNKMKNP
                     GLKIV"
     gene            1656..1886
                     /locus_tag="VLB45_06685"
     CDS             1656..1886
                     /locus_tag="VLB45_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05418.1"
                     /translation="MRYYRRGRNWFSAMGILFITMAGIILIRNLLIWGPDFVGDFFIS
                     SEITNEKISTGMIGFGIFLLYLGFRKKTYEKE"
     gene            1873..2268
                     /locus_tag="VLB45_06690"
     CDS             1873..2268
                     /locus_tag="VLB45_06690"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HSD05419.1"
                     /translation="MKKNEFLKSQKILRLATVDKRGIPHIVPVWYLYKNGKFYVGTNT
                     VTKKARNILKNTKVSFCVDSGIRSPIFGIMGIGTAKVILDKNHVSLLAKKILLRYFKR
                     LNNASAQEILSQTDCIIEIIPKKITVWNY"
     gene            complement(2265..3437)
                     /locus_tag="VLB45_06695"
     CDS             complement(2265..3437)
                     /locus_tag="VLB45_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007549509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HSD05420.1"
                     /translation="MKISKRVEGVEYAIRDIALSARKLEKEGHKITYLNIGDPLQYGF
                     QPPDNVKEALIKAVKSGQNYYAASEGLPELRDAIAKKEKSKGLSVSEDDVIVTNGVSE
                     ALEMLMASIVETGDEVLIPGPYYPPYASYVRLFGGTPIEFSVDLNNSTPDLDDIKSKI
                     TTKTVAICLISPNNPTGAVFNEQSLKSLVDIAIEHDLYIICDEIYDQIVFDEKFVGIG
                     KVSRDAPVILVNGFSKVHLMSGWRIGYLTFNNSKKLEILRENIPKLARVRIASNLPVQ
                     HAALESLRGPQDYIPKFVSELKKRRDYVIKRINGMHGLSCATPKGAFYAFPKIENNKY
                     KSDKEFVMELLKSKGILTVHGSGFGTKYGTGHFRLVFLPEIPILESALDKIEEFVS"
     gene            3481..3555
                     /locus_tag="VLB45_06700"
     tRNA            3481..3555
                     /locus_tag="VLB45_06700"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3516..3518,aa:Arg,seq:tcg)
     gene            3615..4109
                     /locus_tag="VLB45_06705"
     CDS             3615..4109
                     /locus_tag="VLB45_06705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05421.1"
                     /translation="MKTWSSFRIGIVLIIVASIWILIVFSGSEKISSITNLESKESTN
                     LDLDLTKNGIGFYTITIPHYSKHVLFVQILDSNGNVIADKKIATKMTVNYFEFSHGGR
                     YTMEITNVSDEPVEIQALFGNTNASELLIPVFVSFFGAGFIIFSGYKKLSNHNTAQPE
                     ENAT"
     gene            complement(4073..4663)
                     /locus_tag="VLB45_06710"
     CDS             complement(4073..4663)
                     /locus_tag="VLB45_06710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05422.1"
                     /translation="MKLLNTFSHRLASNKKSIVYAISILAVSGTIVELLAGIWDATSH
                     LMREPELFWTIQHVVVYAGVAMITLSAVLGLIILITNQKNTSIHTGLKILIIGAILQI
                     AAGYADSVSHDIYGVDGLVTISHLILETGLMLSALGGFLILSNAGTTKSRIMPLAITS
                     VLLSSTWIGFNLILLFGSVALCLPVYVAFSSGCAVL"
     gene            complement(4714..5016)
                     /locus_tag="VLB45_06715"
     CDS             complement(4714..5016)
                     /locus_tag="VLB45_06715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05423.1"
                     /translation="MTLTVELKGALQQLIDNLMVNPQQMRNMACDLKPLAKDDVEVAF
                     GIFVGYVTGGFAELFFESQKRGMTTPELIEVRKILFERALEMKKAIAYVRAQESQS"
     gene            5130..5369
                     /locus_tag="VLB45_06720"
     CDS             5130..5369
                     /locus_tag="VLB45_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007549503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05424.1"
                     /translation="MSPGIGLMKRRLPTEKEAIPLAVSGIIKKYGVAADQVKTLETKY
                     DEDGGDWYVALGFNEKRAIVKMDSVQGTITEITEI"
BASE COUNT         1760 a          934 c         1002 g         1781 t
ORIGIN      
        1 ttattgtgcc gtcgggtgca aaaagccgta tgattaatcc atcattagga agagctttac
       61 catataccag aagcggttga tcttcggtat acaccttgtt atctgtgaat acctgtaatg
      121 tttcagcatg agctgcagac aaattaatcg atattgccac caatagtaat aatcctgttt
      181 ttattttcaa cttattgaag atgtttcttt cccatagata ttagttctgt taacaaacaa
      241 ggataattgt agaaatatct agaaattaac agcacggttt gactatactt gactatacct
      301 atctgcgttc atagaattaa tagcaacaaa tgaaaatgta ttttgttctt tttttattat
      361 gttaagagat gcattttgta ttattagttc gtataaagat tcaggtttta gctgtaaaat
      421 agtggaaagc atagctttga ttggatccat atgtgtcaca agtagaacat tttgattttt
      481 gtgttgtttt gaacaatagt tgaccatgtc caatacacgt tttttaacac ttgagaatgt
      541 ttcaactcca ttttgtgcaa taacaggatt gtcctgataa aatttcaaaa agacattgcc
      601 atgttttgta aacatatctt cgtatgccat accagagaaa tttcccattt ctatttctgt
      661 cagcctatca tcaactttca aattcagatt gagcttatca gacactattt gtgctgtgtt
      721 tagtgcacgt tcgattgggc ttgagtaaat tgcagaaatt ttaagtgcac caagaaaaga
      781 agctgcatta ttagcttgtt gtaaacccaa atcagtaagc ggaaagcctt ttgtgcgacc
      841 agcaagaatt cgttcaacat tgttcttagc ttgcgcatgt cgcaagaaaa taatgaggga
      901 caagagtatt tcagatgtta aataagagat aataataaca ttaccagagc ttaattttgt
      961 gaaaatagga atcattggcg ggactggagg catgggaaaa ggttttgcac ttagatggtg
     1021 tgttaatcat gatattctga ttggttcacg agatgctgta agagcaacag aggctgcaat
     1081 tgaatattca aaaaatgcgg cagaatttca tcagaatatg aaaggcaaga ttactggtaa
     1141 tgacaatatt tctgttgcga aggaaagtga tgtattggtt ttgtcaatac catacgaaaa
     1201 tatagaatca acctgtgcta ctcttctttc caacataaaa aatacatgta ctgtagtttc
     1261 gcccatagtg ccaatgacaa aaactgatgc aggatttgag tttatttcat tcattgagaa
     1321 aaaaccatct gcgtttgaga tggtacaaaa acacatgaaa aacaaaacaa aattggtctc
     1381 tgcttttcat acagtgtcag agaaaaagct tacagatcca aacctaattt tggattttga
     1441 catatttgtt tgtggagatg atcaagattc aataaatata gtcaatggat tgataaaaga
     1501 aataaaaaat ctcagaccaa tatttcttgg tcctggctcc ctgtcttatc ttgcagaggt
     1561 tgcaacacca atactcttga atgtgatgat aaaaaacaaa atgaagaacc ctggattgaa
     1621 aatagtttaa aattaattat gatcggaaaa ctgttatgag atattatcgt cgtggacgca
     1681 attggttttc tgcgatggga attttattca taacaatggc aggcataata cttatcagaa
     1741 atttgctcat ttggggacct gattttgtag gtgatttttt catatcaagt gagatcacaa
     1801 atgaaaaaat ttctacagga atgatcggat ttggaatatt tctgttatat ttgggattca
     1861 ggaaaaaaac atatgaaaaa gaatgaattt ttaaaatcac aaaaaatatt gcggcttgcc
     1921 acagtagata agagaggtat ccctcatatt gttcctgtct ggtatctata caagaatggc
     1981 aagttttatg ttggaaccaa cactgtgaca aaaaaagcca gaaacatatt aaaaaatacc
     2041 aaagtaagtt tttgtgttga ttccggaatt cgttctccaa tatttggtat aatgggaata
     2101 ggcacagcaa aagtgatact ggataaaaac catgtttctt tactggccaa gaaaattttg
     2161 ttgagatatt ttaagagatt aaacaacgca tctgctcagg agattctctc tcaaacagat
     2221 tgtattatag agataattcc aaaaaaaatt accgtttgga attattagga cacaaattct
     2281 tctattttat caagagcaga ttctagtata ggaatttctg gcaaaaacac caatctgaaa
     2341 tggcctgtcc cgtattttgt tccaaatcca gaaccatgca cagtgagtat gccttttgat
     2401 tttaaaagct ccatgacaaa ttccttgtca gatttgtatt tgttgttttc aatttttgga
     2461 aatgcgtaga aagccccttt tggggttgcg caactgagtc catgcattcc atttattctt
     2521 tttatcacat agtctctacg tttttttaat tcagaaacaa attttggaat gtaatcttgt
     2581 gggcctctga gtgactccaa ggcagcatgt tgaactggca gattagatgc gattctcact
     2641 ctagcaagct ttggtatgtt ttcacgcaag atttcaagtt tttttgaatt attaaaggta
     2701 aggtatccaa tcctccaacc tgacatcaga tggacctttg aaaatccgtt tacaagtatt
     2761 acaggtgcat ctctagaaac ttttccaatt ccgacaaact tttcatcaaa aactatctga
     2821 tcatatattt cgtcacaaat tatgtacagg tcatgttcta ttgcaatatc gactagactt
     2881 ttgagtgatt gttcattaaa aacagcgcct gtaggattat tgggactaat caaacaaata
     2941 gccacagttt ttgtggtaat ctttgatttt atgtcatcta gatctggagt agaattgtta
     3001 agatctactg aaaattctat tggagtacca ccaaacaatc tgacatatga tgcatatgga
     3061 ggatagtaag gacctggtat cagaacttca tcgcccgtct ctactatcga agccattagc
     3121 atctcaagtg cttctgagac gccatttgtt actataacat catcttctga aacggaaaga
     3181 ccttttgatt tttctttttt agcaatagcg tcacgtagtt ctggcaaccc ttcagatgcg
     3241 gcgtaatagt tctgacctga cttgactgct ttgatcagtg cttctttgac attatcagga
     3301 ggctgaaatc catactgtaa cggatcacct atgttgagat atgttatctt gtgaccttct
     3361 ttttctaatt ttcttgcaga aagcgcgata tcacgtattg catattccac gccctctact
     3421 ctttttgata ttttcaatta tctagacaga tatttagccc cgttaaattc ttacttgatc
     3481 ggacccgttg catagccagg atagtgcggg tggcttcgga ccaccaggtc gagggttcga
     3541 atccctccgg gccctttgat tgattctatc aaggtatggt tttgaattta aggcagtata
     3601 caaatagtag aactatgaaa acatggtcat cgtttagaat cggcattgtt ttgataattg
     3661 ttgcttcaat atggatactg attgtatttt caggaagtga aaaaatatca tctattacta
     3721 atttagaatc aaaggagagc accaatctcg atttggattt gaccaaaaat ggaatcgggt
     3781 tttatactat tacgattccc cattattcaa aacatgtttt gtttgtgcag atattagatt
     3841 ctaatggaaa tgtgattgcc gataaaaaaa tagcaaccaa gatgactgtg aattattttg
     3901 agttttcaca tggaggaaga tacactatgg aaataaccaa tgtttctgat gagcctgtag
     3961 aaattcaggc actttttggc aatactaatg catctgaact tttaattcct gtatttgtct
     4021 cattttttgg agctggattt atcatatttt caggatataa gaaattgagc aatcacaata
     4081 ctgcacaacc agaagagaat gccacataga ctggcaaaca caatgcaaca gaaccaaaaa
     4141 gcaagattag attaaaacca atccatgtcg atgaaagaag tacggacgtg atggcaagtg
     4201 gcataattct tgattttgtc gttccagcat tagacaatat cagaaatccg cctaaagcac
     4261 tcagcattaa acctgtttca agtataagat gtgaaatggt aaccagacca tctacgccgt
     4321 aaatgtcgtg cgaaactgaa tctgcataac ctgcagcaat ctgcaatatt gctccaataa
     4381 tgagaatttt caatcctgtg tggatagacg tatttttttg atttgtaatc aaaattatta
     4441 atcctagaac agccgagagc gtgatcattg caacacctgc atatactact acatgttgaa
     4501 ttgtccaaaa caattcaggt tcacgcatga gatgtgatgt tgcgtcccat ataccagcta
     4561 aaagctcaac gatagtacca gaaacagcaa gaattgaaat tgcatatact atagattttt
     4621 tatttgaagc aagtctgtgt gaaaatgtgt ttaaaagttt catctaaatg aaattagtct
     4681 tatgatatta tatagaattt tgtcttgaaa agtttagctc tgactttctt gagcacgcac
     4741 atatgctatg gcttttttca tttccagtgc acgctcaaac aagatcttgc gtacctcgat
     4801 caattctggt gtagtcatgc ctcgtttctg tgattcaaag aacaattctg caaatcctcc
     4861 tgttacgtat ccgacaaata ttccaaatgc cacttcgaca tcatctttag caagaggttt
     4921 caagtcacat gccatgtttc tcatttgctg tggatttacc attaaattat ctattagctg
     4981 ctgaagtgca ccttttagct ctacggttag tgtcatcaat ttttctaatc accgtttaca
     5041 aaagaacctt accagatcat atcttatgac aaaatttcga tcttttttga ctcctcttaa
     5101 ttattatacc acatgagaga agagatttta tgagtcctgg aattggcttg atgaagagga
     5161 gacttcctac tgaaaaagag gcaatcccat tggcagtttc tggaataata aaaaaatatg
     5221 gagttgctgc agaccaagtc aaaacacttg aaacaaaata cgacgaagat ggtggagatt
     5281 ggtatgttgc tctcggattt aatgaaaaaa gggctattgt taaaatggat tcagtacagg
     5341 gaacaataac agaaattaca gaaatctagt aaaaattaat ttatctttta aattgtacgt
     5401 tagaaacttc gggttcattg ataatgatca acccgccatc ccgcagttgt tggattagct
     5461 gtataacttc cttttct
//
LOCUS       DATIDL010000149         3457 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrosopumilaceae archaeon isolate SMAG_U16342
            k141_2812015, whole genome shotgun sequence.
ACCESSION   DATIDL010000149 DATIDL010000000
VERSION     DATIDL010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935836
            Sequence Read Archive: SRR25158326
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosopumilaceae archaeon (soil metagenome)
  ORGANISM  Nitrosopumilaceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrosopumilales;
            Nitrosopumilaceae.
REFERENCE   1  (bases 1 to 3457)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3457)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/04/2024 05:09:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,520
            CDSs (total)                 :: 1,474
            Genes (coding)               :: 1,474
            CDSs (with protein)          :: 1,474
            Genes (RNA)                  :: 46
            rRNAs                        :: 1 (5S)
            complete rRNAs               :: 1 (5S)
            tRNAs                        :: 43
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3457
                     /organism="Nitrosopumilaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2812015"
                     /isolate="SMAG_U16342"
                     /isolation_source="soil"
                     /db_xref="taxon:2202732"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..489)
                     /locus_tag="VLB45_06725"
     CDS             complement(<1..489)
                     /locus_tag="VLB45_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007401776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HSD05425.1"
                     /translation="MSKLAPLPPAPVLMTCFGHCGIGLGAESYRRLLLDVGADPLRPT
                     LKNEKDESRVLKRYGSTILRFPNTYAGGEIPLIPRALLIDLDPRAANLILQSYPDLFA
                     LRDKHVIHGAGGAARNWAEGRTRFIKEVSTKYDMQKQLSALSPEPVRGFTVPFAMGGG
                     TGS"
     gene            complement(648..1007)
                     /locus_tag="VLB45_06730"
     CDS             complement(648..1007)
                     /locus_tag="VLB45_06730"
                     /inference="COORDINATES: protein motif:HMM:NF021378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HSD05426.1"
                     /translation="MSEDLIKTVQNLIDSKKGDRKRLNEILDTLKQGTPLYLSDYRYV
                     ESLNSKSKDDLKEDSNLNAQQNQSKKIERDVGIDLDDLKPKDDDDAMTILRTRLANGE
                     ISIEEFRTIKKALQDNM"
     gene            1071..1742
                     /gene="tpiA"
                     /locus_tag="VLB45_06735"
     CDS             1071..1742
                     /gene="tpiA"
                     /locus_tag="VLB45_06735"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007550738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HSD05427.1"
                     /translation="MFIINFKNYEEIAGTRAIKLAKIAEKISKKYKIKIAIAPPHHLL
                     SELVKYHIPVLAQHFDIASVGSTTGFMVPEIVKKSLVKGSLINHSEHRIPENQIKKLV
                     LRLKKLGMISVVCVKDVNEARKYAKLDPDYIAIEPPELIGSGKAVSKERPEIITKSVS
                     AVRQARNSTKLLCGAGIVSGDDVAKAVQLGSKGILVASGIVKAKNWNKTIEEFAIAIS
                     KNQKG"
     gene            1770..>3457
                     /gene="ppdK"
                     /locus_tag="VLB45_06740"
     CDS             1770..>3457
                     /gene="ppdK"
                     /locus_tag="VLB45_06740"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HSD05428.1"
                     /translation="MKSIYFFDEGDGKNKKLLGGKGAGLCEMTRIGLPVPPGFTITTE
                     VCDKYYQNNKKLPGNLMAEVRRNIAKIEKRTGKKFGSIENPLLVSVRSGAALSMPGMM
                     DTILNLGLNDQTVTGLAKKSNNERFAWDSYRRFIQLFGKVVFGVDDKKFDEVLEKAKH
                     TQGIQLDSDLNVQSLKSVVAQYKQICETQLGKPFPADPYKQLELAIEAVFRSWMGERA
                     VVYREKYKITKDIANGTAVNIVTMVFGNMGNDSATGVVFTRNPGDGTRKVFGEYLVNA
                     QGEDVVAGIRTPQTIDNLARELPQTNSQLMKTCEKLERHYKEPQDLEFTVEQGKFYML
                     QTRSAKMNAIGMIRTSVEMVKEKLITKERALTRLQPDQLEQLLHKTINQEAAKNHSLL
                     VKGIAASPGAATGVVVFDVKKAVTMGDDGVKVILVREETKPEDVPAFFASVGILTSRG
                     GKTSHAAVVARGMGKPCIVGCSEMKIDYASKKFQVDSRIVNEGEVITIDGSNGSVFVG
                     EIPTIEPEMTGEFKTILGWAEQTKRLDIRANADTPEAARLARSYGAKGIGLCRT"
BASE COUNT         1161 a          640 c          732 g          924 t
ORIGIN      
        1 gctgcctgtt cctcctccca tggcaaacgg gactgtgaaa cccctaacag gctctggtga
       61 aagtgcagaa agttgttttt gcatatcata ttttgtgctt acttctttga tgaatctggt
      121 tcttccctct gcccaatttc ttgcggcacc accagcgcca tgaattacat gcttgtcacg
      181 taatgcaaaa agatcaggat aagattgcag tataagattg gcagctcgtg gatccaagtc
      241 aataagcaaa gcccttggaa tgagcgggat ttctccacca gcatatgtat ttggaaatcg
      301 caaaatcgtg ctaccatatc ttttcaatac tcttgattca tctttttcat ttttcaaggt
      361 aggtcttagt ggatctgcgc caacatcaag caataatctt cgataagact ctgcaccaag
      421 tcctattcca caatgaccaa agcaagtcat caagactggg gctggtggaa gcggagctag
      481 ctttgacatg ttatcaacct aacagagagg aacgttctct ctttaaccga atcatacaat
      541 attgtaaagt gctgacttga ttgttataga catctaattg attcatactt gttctatcct
      601 atgatagttt aattcgatta catttaagat ttgagaagaa atcatcctta catgttgtct
      661 tgtaatgctt tttttatggt tctaaattct tcaattgaga tttcaccgtt tgcaagcctg
      721 gttctcagta ttgtcattgc atcatcgtca tccttgggct tcaagtcgtc aagatctatt
      781 ccaacatcac gctctatttt tttggattgg ttctgttgtg cgtttagatt actatcttct
      841 tttagatcat ccttactttt agagttcaaa ctttcaacat acctatagtc tgacagatac
      901 aaaggagtgc cttgcttgag cgtatctaga atttcgttca atctttttct atcgcctttt
      961 tttgaatcga ttaggttttg aacggttttg atcaaatctt cagacaattt tttcttgatt
     1021 attttaggat tcaatctata tatctaggat gctctctaag tttctctagt ttgtttataa
     1081 ttaattttaa aaattatgaa gaaattgccg gcactcgtgc aatcaaactg gcaaaaattg
     1141 cagaaaagat ttcaaaaaaa tacaagatca aaatagctat tgcgccacct caccatctgc
     1201 tatctgaact ggtcaaatac cacataccag tactagccca gcattttgat atagcaagcg
     1261 ttggcagcac caccggattt atggttccag agattgtaaa aaaatcccta gtcaaaggat
     1321 ctctgataaa ccatagcgaa cacaggattc cagaaaatca gataaaaaaa ttggttttaa
     1381 gactcaaaaa acttggcatg atttcagttg tatgcgtcaa agatgtaaac gaggcaagaa
     1441 aatatgcaaa acttgatcca gattatatcg caatagagcc acctgaatta attggaagcg
     1501 gaaaagcagt atcaaaagaa agaccggaaa tcataacaaa atcggtatcg gcagtaaggc
     1561 aggcaagaaa ttctaccaaa ctactttgtg gggcaggcat tgtatctggt gatgacgtag
     1621 caaaggctgt gcagcttggc tcaaagggaa tacttgtggc aagcggcatt gtaaaagcaa
     1681 aaaactggaa taaaacaata gaagaatttg ccattgcaat ttcaaaaaac cagaaaggtt
     1741 gataacccaa acgggacaat gggcgtaata tgaaatcgat ttattttttt gacgaaggag
     1801 acggcaagaa caagaaattg cttggtggaa agggggctgg cctatgcgag atgacaagaa
     1861 tcggactgcc tgtgcctcca ggatttacaa taactacaga ggtttgtgac aagtattatc
     1921 agaacaataa gaaactgcct ggcaatttaa tggctgaagt aagaagaaac attgccaaga
     1981 tagaaaaaag aactggtaaa aaatttggtt ccatagagaa tccacttttg gtatcagtaa
     2041 gatcaggtgc tgcactttcc atgcctggca tgatggatac cattttgaat cttggattaa
     2101 acgatcaaac tgttacgggt cttgcaaaaa aaagcaataa cgagcgattt gcttgggatt
     2161 cttatcgtag atttatccaa ctttttggca aggttgtttt tggagttgat gataaaaaat
     2221 ttgacgaggt tttagaaaaa gcaaaacaca ctcaaggtat tcagcttgat agcgacctga
     2281 acgtacagtc gctgaaaagt gttgtcgcac aatacaaaca gatttgtgag actcagcttg
     2341 gcaagccatt tcctgcagat ccatacaagc agttagagct tgcaatagag gcagtcttca
     2401 gaagctggat gggtgagaga gctgtagtgt atagagaaaa atacaaaatt accaaagata
     2461 tcgcaaatgg aactgcggtg aatatagtca ccatggtttt tggcaacatg ggaaacgaca
     2521 gtgccacagg cgtagttttt actagaaacc caggagatgg aacaagaaaa gtctttggag
     2581 aatatcttgt caatgcacaa ggagaagacg ttgttgcagg aattagaaca ccacaaacaa
     2641 tagacaattt ggcaagagaa ctaccgcaga caaactcaca gcttatgaaa acttgcgaaa
     2701 aactggaaag gcactacaaa gaaccacaag atctggaatt taccgtagaa caaggaaaat
     2761 tctacatgtt acaaacccgt tctgcaaaaa tgaatgcaat tggaatgatt agaacgtcag
     2821 tagagatggt aaaagaaaaa ctcatcacaa aagaaagagc cctaacaaga ctgcagccag
     2881 atcagctaga acaactatta cacaagacaa taaaccaaga ggccgcaaag aatcattcgc
     2941 ttctagtcaa agggatagct gcatctccag gcgcagcgac aggagtggtt gtgtttgatg
     3001 taaaaaaagc agttacaatg ggagacgacg gagtcaaagt aattcttgtc agggaagaaa
     3061 caaaaccaga ggatgtgcca gcattttttg catcagtagg cattcttaca agcagaggcg
     3121 gaaagacatc acatgcagca gtggttgcac gcggcatggg caagccatgc attgtaggat
     3181 gctctgagat gaaaatcgat tatgcatcaa aaaaattcca agtagatagc agaatagtaa
     3241 atgaaggcga agtcatcact atagacggca gtaatggaag tgtgtttgtt ggcgaaattc
     3301 ccacaataga gcctgaaatg actggtgaat tcaagacaat acttggatgg gcagaacaaa
     3361 ccaagagatt agacattaga gcaaatgcag acaccccaga agctgcaaga ctagccagga
     3421 gttatggtgc aaaaggcata ggtctttgca gaacaga
//
LOCUS       DATIDL010000150         3387 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrosopumilaceae archaeon isolate SMAG_U16342
            k141_2202616, whole genome shotgun sequence.
ACCESSION   DATIDL010000150 DATIDL010000000
VERSION     DATIDL010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935836
            Sequence Read Archive: SRR25158326
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosopumilaceae archaeon (soil metagenome)
  ORGANISM  Nitrosopumilaceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrosopumilales;
            Nitrosopumilaceae.
REFERENCE   1  (bases 1 to 3387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3387)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/04/2024 05:09:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,520
            CDSs (total)                 :: 1,474
            Genes (coding)               :: 1,474
            CDSs (with protein)          :: 1,474
            Genes (RNA)                  :: 46
            rRNAs                        :: 1 (5S)
            complete rRNAs               :: 1 (5S)
            tRNAs                        :: 43
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3387
                     /organism="Nitrosopumilaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2202616"
                     /isolate="SMAG_U16342"
                     /isolation_source="soil"
                     /db_xref="taxon:2202732"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1166
                     /locus_tag="VLB45_06745"
     CDS             <1..1166
                     /locus_tag="VLB45_06745"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LamG-like jellyroll fold domain-containing
                     protein"
                     /protein_id="HSD05429.1"
                     /translation="TKQTLVRWEKNGGSPVKYYEISVPGTGSNAGKIVFEFNTQSTGS
                     GEITTCVSNNAIKRVDDGNWHHVVAVRKDGSGNPQQKCELYIDGILHSSVQSPHNTPN
                     MEADGKWYVGSNRAENADFFKGRIDDIIHWNSKALDATEVLDLFKTSYGENAHKVTFK
                     IDKTDKDGNLIQNLRTNSSYPLDFLDGDGKYNSPSAADWKWFNFTTPTIDSASLTNQR
                     IRFNMTFNHPLLNNGELDMKLRIDDVGVTSGLGSSFLQIPMPDQTFPGYYTYDNDNFP
                     LVTVVNNSPNGAWISFLTRAVFDSLDTSSSYGALIKRVDTTTLSASNDSPFFVPSMSH
                     DVEFDKPYAIPGSSSSGIVPPGRYKLNVFLSGYDEYGLIYLQTSYIGVVRVVD"
     gene            1226..1720
                     /locus_tag="VLB45_06750"
     CDS             1226..1720
                     /locus_tag="VLB45_06750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05430.1"
                     /translation="MSTNSNGQPEGFSNTLKYLDSLVDEALQVYELDNEKTNIVDELY
                     HSLKVITGFLGFSVDLSPDILNLPSDTRITLTSSLDVLVIRSNGKSEQKRLEEFSFEE
                     ILRILEYSIPNMIGLIRTERTTMNEKITFLREATKKLQHMHNLNPQKHEISNTTVPLE
                     EVKR"
     gene            1721..2284
                     /locus_tag="VLB45_06755"
     CDS             1721..2284
                     /locus_tag="VLB45_06755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05431.1"
                     /translation="MTIEDIKKFKTLEEMQIIVEKELDTLKNKSEKYSKIIGDKLREN
                     QDPAHDSDLAELKEKLDGPADPKKKKTVKKDKKSNWHDFDGISVFDGIGLKGELEIYF
                     KALEETKSKIETLGKTMDAINNLISKGMRRDLGCVALIRHNLPSEMVFVKSNIVDEKK
                     FSFKSIFSVYTEPIETDKSNTYSLNRV"
     gene            2421..3281
                     /locus_tag="VLB45_06760"
     CDS             2421..3281
                     /locus_tag="VLB45_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HSD05432.1"
                     /translation="MKEEQIQEATVLDSMPDSSMYDYRISLDKLRDQLATFGLTANQC
                     KVYIFLGKYGSKTAPEVCKALKLPRTETYHLLTTLQNKGIVSATFQHPIRFSALPLGK
                     AVWVLVNAEKERVRTLETQEKELVELWNNIPDFHAEPTEPVEDKFQMLQGINQIHGKI
                     KEMITNAVSEFYVLGSEKDYLKFYHSDFLSPLDNSEVDLKLLTSSSDRTMYIFDEVDG
                     SKVKKIPDGIRDNLCFIIKDNDELLFFMKNANQADQQIMAMWTNSSPMIHSMKLLFGY
                     VWSKSKYVTA"
BASE COUNT         1271 a          558 c          622 g          936 t
ORIGIN      
        1 caacaaaaca gacgttggtt cgctgggaaa aaaatggtgg cagtccagta aaatattatg
       61 agatatctgt tccaggaaca ggttcaaatg caggtaagat agtatttgaa ttcaatacac
      121 agagtactgg gtccggagaa ataacaactt gtgttagtaa caatgcaatc aaaagagttg
      181 atgatggaaa ctggcatcat gtagtagcag taagaaagga tggttcaggt aacccacaac
      241 aaaaatgtga attgtatatt gatggtatac tccatagttc tgttcagagc ccacataaca
      301 cacctaacat ggaagcagat ggaaaatggt atgtaggaag taacagagct gagaatgctg
      361 acttttttaa aggcagaata gatgacataa ttcactggaa ctcaaaagca ttagatgcca
      421 cagaagtctt ggatttgttt aagacaagct atggtgaaaa tgcacataaa gtcacattta
      481 aaatcgataa gactgataaa gatggaaacc ttatccaaaa tttgagaacc aacagcagct
      541 atcctttaga ctttctagat ggggatggta agtacaacag cccctcagct gcagattgga
      601 aatggtttaa cttcacaaca ccaacaattg atagtgcatc cctaacaaat cagagaatac
      661 gattcaacat gacttttaat catccacttt tgaataacgg agaactagac atgaagttac
      721 gcatagatga tgttggtgta acttctggtc tgggaagttc ttttcttcag attccaatgc
      781 cagatcaaac ttttccagga tattacacat atgataatga caacttccca ttggtcacag
      841 tagtgaataa tagtcctaat ggagcctgga tatcatttct aactcgggct gtttttgaca
      901 gcttggatac aagttcatca tatggagcat tgataaaacg agttgatact accacattat
      961 ctgcaagtaa cgacagcccc ttttttgtac catcaatgtc acatgacgtt gaatttgata
     1021 aaccatatgc aatacctggt tcatcgtcaa gtggaatagt tccacctggc aggtataagt
     1081 tgaatgtgtt cctcagcgga tatgatgaat atggtttgat atatcttcaa acctcgtata
     1141 ttggggtagt acgtgtggta gattagaagg ttgtaacatc aacttacata ctacctttct
     1201 tgtattaaaa aagcagtcta agttcatgtc aacgaatagc aatggacagc ctgaaggttt
     1261 ttctaacacc ctaaaatatc tagattcctt agttgacgaa gcacttcagg tgtatgaact
     1321 agataatgaa aaaacaaaca tagtagatga gctatatcat tcattaaaag taatcactgg
     1381 atttttagga ttttcagttg atctttctcc agacattttg aatttgccgt cagataccag
     1441 aataacactt acttcgtcat tagatgtatt agtcataaga tcaaatggca aatctgaaca
     1501 aaaaagatta gaagaatttt catttgaaga aatattacga atactagaat attctattcc
     1561 aaacatgata ggcttgataa gaactgaaag aactacaatg aatgaaaaaa ttacatttct
     1621 acgagaggct accaaaaaat tacaacatat gcataatcta aacccacaaa aacatgaaat
     1681 ctctaatacc acggtaccac ttgaagaggt caaacgttaa atgactatag aagatataaa
     1741 aaaattcaaa actctagaag aaatgcaaat tatagttgaa aaagaattag atactttaaa
     1801 aaacaaatca gaaaaatatt ccaaaataat tggtgataaa ctaagagaaa atcaagatcc
     1861 tgctcatgat tcagatttgg cagaattaaa agagaaacta gatggacctg cagacccaaa
     1921 aaagaaaaaa actgtgaaaa aagacaaaaa aagcaactgg catgattttg acggaatatc
     1981 cgtctttgat ggcattgggt taaagggtga attagaaata tactttaaag ctttagaaga
     2041 aacaaaatca aagatagaaa ccttaggtaa aacaatggac gcaataaaca atctaatctc
     2101 aaagggcatg agacgagatt taggctgcgt tgcattgatt agacataatt tgccttcaga
     2161 gatggtgttt gtaaaatcaa acatcgtaga tgagaaaaaa ttctcattta aatcgatctt
     2221 ttctgtttat actgagccga ttgagacaga taaatctaat acatatagtc taaatagggt
     2281 ataaaatatg caaaaaacat ataatttttt aataaaattc acatataagc gatcaactga
     2341 tctatcagtt cgatcaagat tagttaactt gcgtacaata tatccaaccg gtttgatcaa
     2401 cagatttcta agggaaaaaa atgaaagaag aacaaataca ggaggcaaca gttttggatt
     2461 ctatgcctga ctctagcatg tatgactaca ggatatcact ggacaagctc agagatcaac
     2521 tagcgacgtt tggtcttaca gcaaaccaat gcaaggtata catatttcta ggcaaatatg
     2581 gttcaaagac agctcccgaa gtatgcaaag ctttgaaatt accaagaact gaaacatatc
     2641 atttactaac tactttacaa aacaagggaa tagtttctgc aacattccaa catcccatta
     2701 ggttttcagc tctgccgtta ggtaaagctg tatgggtatt agtgaatgca gaaaaggaac
     2761 gtgttagaac acttgaaaca caagaaaagg aactggtaga attatggaat aatattccag
     2821 acttccacgc agaaccaaca gaaccagtgg aagacaaatt ccaaatgcta caagggataa
     2881 accaaataca cggcaaaata aaagaaatga tcacaaatgc agtaagcgaa ttttatgttt
     2941 tgggctctga aaaagattat ttaaaatttt atcattccga tttccttagc ccgttggaca
     3001 actcagaagt ggatctcaaa ctgttgacgt catcatcgga tagaacgatg tatatctttg
     3061 acgaagttga tggaagtaaa gttaaaaaga ttccagatgg aataagagac aatctttgtt
     3121 tcataattaa agacaatgat gaattacttt ttttcatgaa gaatgcaaac caagcagatc
     3181 aacaaataat ggcaatgtgg acaaattcta gtccgatgat tcattcaatg aagctgttgt
     3241 ttggttatgt ctggtctaaa tcaaagtacg taacagcata attttacgaa accaatcttg
     3301 gatgtggaat caatattcct gattctccta atttgacagg atgaatttgt tcactatgtc
     3361 gcataccgcg catcttaatt acctgta
//
LOCUS       DATIDL010000151         2526 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrosopumilaceae archaeon isolate SMAG_U16342
            k141_1598659, whole genome shotgun sequence.
ACCESSION   DATIDL010000151 DATIDL010000000
VERSION     DATIDL010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935836
            Sequence Read Archive: SRR25158326
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosopumilaceae archaeon (soil metagenome)
  ORGANISM  Nitrosopumilaceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrosopumilales;
            Nitrosopumilaceae.
REFERENCE   1  (bases 1 to 2526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2526)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/04/2024 05:09:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,520
            CDSs (total)                 :: 1,474
            Genes (coding)               :: 1,474
            CDSs (with protein)          :: 1,474
            Genes (RNA)                  :: 46
            rRNAs                        :: 1 (5S)
            complete rRNAs               :: 1 (5S)
            tRNAs                        :: 43
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2526
                     /organism="Nitrosopumilaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1598659"
                     /isolate="SMAG_U16342"
                     /isolation_source="soil"
                     /db_xref="taxon:2202732"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..421
                     /locus_tag="VLB45_06765"
     CDS             <1..421
                     /locus_tag="VLB45_06765"
                     /inference="COORDINATES: protein motif:HMM:TIGR04296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PEFG-CTERM sorting domain-containing protein"
                     /protein_id="HSD05433.1"
                     /translation="QTSLDGTVLVTIQTTDATAGEYLGIDVIFKDSSEKQIPHVNYDL
                     KVSQEGNDVLFLKSQHAEAGMVEHWTRPLFSDKPVDVEVTLLGMGLPKDRESWTGQSG
                     ETLSFYAVPEFGTLALAILIISILSVIALTKPLTRKL"
     gene            complement(424..900)
                     /locus_tag="VLB45_06770"
     CDS             complement(424..900)
                     /locus_tag="VLB45_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PUA domain-containing protein"
                     /protein_id="HSD05434.1"
                     /translation="MKSNLLSKSDTASALEDIAVQWKIDIPKIKNLTIHEIEDDASLM
                     TAEGFAAIKIGQTYIPFLSETQTLEKFPKVIVDMGAIKFVCNGATVMRPGIKSFTEFE
                     KDQIVCVVEESKNKFLAVGRSLVSSKEMSTMTKGEAIKNLHHISDRFWEAAKEIKK"
     gene            complement(897..1907)
                     /locus_tag="VLB45_06775"
     CDS             complement(897..1907)
                     /locus_tag="VLB45_06775"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008300501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HSD05435.1"
                     /translation="MRIILSGFGVVGQNFAKLLLSRSEDLYALHGLKPRIVGVFDSKG
                     SAASSAGLDLNRLLEMKKKYGSIKKYHNKEKDANGLEMIRGMEAEVLIETTPSNYKDA
                     EPGMSHIVNAMKHGLHVITVNKGPLALAFPSLIELATYNQVLLKFSGTVGGGTPILDY
                     AKNSLRGERIISFQGILNGTTNYILTNMAGGMTFKAALADAKKKGYVEADESLDIDGF
                     DAAAKLVILANWIMDMKVTIKDILRKGIRDVSTQDIKKAASKNCAVKLVASCNKDLVV
                     SPREIPLDDPLCVNGTLNAITFNSEHSGQQTIIGRGAGGMETASSILRDLLDIKQEMS
                     RH"
     gene            2003..>2526
                     /locus_tag="VLB45_06780"
     CDS             2003..>2526
                     /locus_tag="VLB45_06780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05436.1"
                     /translation="MKSNKYLYAGTGAAAALAVIIIVSYSVTPTQSVPEIDSLALDFT
                     YDEANSDLKSNLISEGISMSSPLKFVEQNSIEKYCSFFSDPEKQKLVEYCTSTELKDG
                     NGNFLGNIHMIGTIDAPRLVFVVVQSNPVDTGQVKTVFGISTKELVCDCWNQVKPGGY
                     ETIESWIDALRDFHT"
BASE COUNT          717 a          508 c          473 g          828 t
ORIGIN      
        1 gcaaacatca cttgacggaa ctgttcttgt taccatacaa actacagacg caacagctgg
       61 tgaatacttg ggaatagatg taattttcaa agactcgagt gaaaaacaaa tcccacacgt
      121 aaactatgat cttaaagttt cacaagaagg aaatgatgtc ctatttttaa aatcgcaaca
      181 tgcagaagct ggtatggtag aacactggac tagaccgcta ttctcagaca aaccagtaga
      241 tgttgaagtg acacttcttg gaatgggatt gccaaaagat agagaaagct ggactggcca
      301 aagcggagag actctgtcct tttatgctgt acctgaattt ggaactttgg cactagcgat
      361 tctaataatt tcaatacttt ctgtgattgc actaaccaaa cctttgactc gcaaactcta
      421 agattatttt tttatctctt ttgcagcttc ccaaaatctg tctgatatgt ggtgtaggtt
      481 ttttattgct tcaccttttg tcatcgtaga catttccttg cttgatacaa gtgatcttcc
      541 tactgccaaa aacttgtttt ttgattcctc taccacgcaa actatctggt ctttttcaaa
      601 ctctgtaaag ctcttgatgc caggcctcat cacggtagca ccattgcaga caaattttat
      661 tgcgcccatg tctactatca ccttgggaaa tttctcaagt gtctgtgttt cagacaaaaa
      721 tggaatgtat gtttgaccta tttttattgc agcaaagcct tctgcagtca ttaaactggc
      781 atcatcttct atttcatgaa tagtgagatt ttttattttt ggaatatcga ttttccactg
      841 tactgcaata tcctcaagtg cactggcagt atcacttttt gatagtaaat ttgatttcaa
      901 tgtcttgaca tttcctgctt gatgtctagt aaatctcgca gaatcgagct tgctgtttcc
      961 atgccgcctg caccacgacc aattattgtc tgctgccctg aatgttccga attaaaggtg
     1021 attgcattga gtgtgccatt cacacataat ggatcatcta aaggaatttc tcgtggtgat
     1081 actaccagat ctttattaca cgaagcaaca agcttgactg cgcaattttt ggaagctgcc
     1141 tttttgatat cttgtgttga cacatcgcgt attccttttc gtaggatgtc ttttattgtg
     1201 actttcatgt ccattatcca attggcaagt atcacaagtt ttgcggctgc atcaaatccg
     1261 tctatatcta atgattcgtc tgcctccacg tatccttttt tctttgcatc tgcaagtgcg
     1321 gccttgaatg tcataccacc tgccatgttt gtaaggatgt aatttgtggt gccgtttaaa
     1381 attccctgaa atgaaatgat tctttctcca cgaagactgt tttttgcata atccaaaatg
     1441 ggggtgcctc ctccaacggt gccactaaat tttagcaaaa cctgattgta tgtggcaagc
     1501 tctatcagcg atggaaatgc aagggcaaga ggtcccttgt ttacagtgat cacatgcagt
     1561 ccgtgtttca ttgcgtttac aatgtgagac atacctggct cggcatcttt gtaattgctt
     1621 ggagttgtct caattaggac ttcggcctcc attcctctta tcatctcaag accgtttgcg
     1681 tctttttctt tattgtgata ttttttaatg ctgccgtatt tttttttcat ctctagcaat
     1741 ctgttaagat ccagtcctgc tgaggatgca gcagagcctt tgctgtcaaa gacgcctaca
     1801 attcttggct ttaacccatg caatgcataa agatcctctg accttgaaag caataacttt
     1861 gcaaagttct ggccaacaac accaaaccct gataatatta ttcgcaacaa tcaacaagcg
     1921 taatacggta ttaattaaga ttgagagttt ttgaatgaaa atatcagatt cctttattaa
     1981 ataacaaaaa acttgagaga tcatgaagtc aaacaaatat ctttatgctg gaacaggtgc
     2041 agcagctgct cttgctgtaa tcataattgt gtcgtattct gtgactccaa ctcaatctgt
     2101 accagaaata gactcacttg cattggattt tacatatgat gaagctaatt ctgatttgaa
     2161 atcaaattta atatcggaag gaatctcaat gtcttctcca ttaaagtttg ttgaacaaaa
     2221 cagcattgaa aaatattgta gttttttcag tgatccagaa aaacaaaagc ttgttgaata
     2281 ttgcacgtca acggaactaa aagatggaaa cggaaatttt cttggaaata ttcacatgat
     2341 aggaacaatt gatgccccgc gattggtctt tgttgtagtg cagtcaaacc cagttgatac
     2401 cggccaagta aagacggtat ttggtatatc cacaaaggaa cttgtttgtg attgctggaa
     2461 tcaagtaaag ccaggtggat atgagacaat tgaaagctgg attgatgcct tgcgagactt
     2521 tcatac
//
LOCUS       DATIDL010000152         3292 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrosopumilaceae archaeon isolate SMAG_U16342
            k141_2415008, whole genome shotgun sequence.
ACCESSION   DATIDL010000152 DATIDL010000000
VERSION     DATIDL010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935836
            Sequence Read Archive: SRR25158326
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosopumilaceae archaeon (soil metagenome)
  ORGANISM  Nitrosopumilaceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrosopumilales;
            Nitrosopumilaceae.
REFERENCE   1  (bases 1 to 3292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3292)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/04/2024 05:09:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,520
            CDSs (total)                 :: 1,474
            Genes (coding)               :: 1,474
            CDSs (with protein)          :: 1,474
            Genes (RNA)                  :: 46
            rRNAs                        :: 1 (5S)
            complete rRNAs               :: 1 (5S)
            tRNAs                        :: 43
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3292
                     /organism="Nitrosopumilaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2415008"
                     /isolate="SMAG_U16342"
                     /isolation_source="soil"
                     /db_xref="taxon:2202732"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..398
                     /locus_tag="VLB45_06785"
     CDS             <1..398
                     /locus_tag="VLB45_06785"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HSD05437.1"
                     /translation="AIAISKRFGSVLYLLTVVDISTIRPPGMLLGIVLNKKMKKLSSK
                     ISESIILKTNKRLKNEVEFCRKNDVKTYYQIMKGYAVDAILKFALKNNIDLIVMGSKG
                     LSGFGKLLTLGSVSRRVSEEANCPVMLVR"
     gene            667..1419
                     /locus_tag="VLB45_06790"
     CDS             667..1419
                     /locus_tag="VLB45_06790"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HSD05438.1"
                     /translation="MVLDGIIGLAPISKRHGHYTEVRSVIVPTDSVKLHGDLTIPKNA
                     EGIVLFAHGSGSSRHSTRNKHVAQVLQKAGLATLLIDLLTEDEEKIDDYTMHLRFDIE
                     LLAKRLAGATSWLTKNPDTKNLNVGYFGASTGAAAALVASVDCQNLIKAIVSRGGRPD
                     LAGSVLPRVKAPTLLIVGGNDSPVIEMNEEALNLLTTEKKIVIIPDATHLFEEPGTLD
                     EVARLAADWFVKHLTKTKNSIKSKKETAVGVS"
     gene            1406..2077
                     /locus_tag="VLB45_06795"
     CDS             1406..2077
                     /locus_tag="VLB45_06795"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HSD05439.1"
                     /translation="MESADSYMVFKDRVDAAQRLAKKLEWLKKEDPIILAIPRGGVVT
                     GDVISSELGVKLDIIVSRKVVAPSNPELAIGAVTHDGSYFPNVDITRMLNIPQKYIDA
                     EISVQMKEIERRLMRFRGSKEYHLKGRTVVLVDDGIATGATMFVAIQWVKKQKPKQLI
                     VAVPVGPKETIDKLNEVADKVVVLQSPVYFNAVGEFYQEFNQVDDYQVQEIMSKHGYK
                     IQIFK"
     gene            2130..2969
                     /locus_tag="VLB45_06800"
     CDS             2130..2969
                     /locus_tag="VLB45_06800"
                     /inference="COORDINATES: protein motif:HMM:NF015442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP cone domain-containing protein"
                     /protein_id="HSD05440.1"
                     /translation="MKHDGKKIYVIKANGQSVPFDGNKVKATCMRAGASNNLAERVTQ
                     KVHEQLHHGIRTREIYRMVLAALASEDEGRFIKHRYRLKESIMLMGPAGFAFEIYVGK
                     ILENYGYHVESTRSQVNGKCVKHEIDLIVDSNLTSERYMVECKYHNFPGIYTGIKESL
                     YTHARFLDLSEVFDNEMLVCNTKVSDDVITYAKCVGQKLLCWRYPAKKGLENMIEEKG
                     LYPITILGLSAKELLSLSQYKIMLAKDLLTIDIDQLARKIDMSYTRLHRLQNLVQNIL
                     YRN"
BASE COUNT         1203 a          505 c          668 g          916 t
ORIGIN      
        1 aagcaattgc aatttcaaaa agatttggtt ctgtactata tttactaact gttgttgaca
       61 tttcaacaat aagaccacca ggcatgttgc ttggaattgt attgaataag aaaatgaaaa
      121 aactgtcatc aaaaatctca gaatcaataa ttttaaaaac aaataagcgt ttgaaaaacg
      181 aagtagagtt ttgcagaaaa aatgatgtta aaacatatta tcaaataatg aagggatatg
      241 cagtcgatgc catattgaaa tttgcattaa aaaacaatat tgacctaatt gtgatgggca
      301 gtaaagggct gtcaggattt ggtaaactcc tgacattagg aagcgttagc aggagagtat
      361 ctgaagaggc caattgccca gttatgttgg tacgataacg gcatggcata caagaacatg
      421 cttattctac tacaattgtt ccgtggctga ttatcagtat tgaatttctg aaaatcagtt
      481 ttaaatgtta attgagaaaa tctctcaacc aactaagcta tgaataaaca agatcacaaa
      541 accaatgata aatggaataa ccaaatatca agagacagtg cgtgtacttg tcaaaggtgt
      601 gatcatggtc tagctagaga ctgtatgaag gtaaaatgta catgttgtag ccaagaaagt
      661 cattcaatgg ttctagatgg aattatagga ttggcaccaa taagcaaaag acatgggcat
      721 tataccgaag tacgttccgt aatagtcccg actgactctg ttaaactaca tggagacttg
      781 accataccaa aaaatgcaga agggattgta ttatttgcac atggcagcgg aagtagtaga
      841 cacagtacta gaaataagca cgttgctcaa gttcttcaaa aagctggtct tgctacgcta
      901 ttgattgatc ttttaactga agatgaagaa aaaatagatg attatactat gcatctcagg
      961 tttgatattg agttgttagc aaaacgatta gctggtgcta ctagttggtt aacaaaaaac
     1021 cctgacacta aaaatcttaa tgtcgggtat tttggtgcaa gcacaggtgc agctgcagca
     1081 ttagttgcgt cagtggattg tcagaatctc attaaagcaa ttgtctcaag aggcggacgg
     1141 ccagatttag ctggttctgt attaccacgt gttaaagctc caactttact catagttggt
     1201 ggaaatgaca gccctgttat agaaatgaat gaagaagcat tgaatctgct tacgactgaa
     1261 aaaaaaattg taataattcc tgatgccacg catctgtttg aagaaccggg aacacttgat
     1321 gaagttgcac gtcttgcagc tgattggttt gtaaaacatc taactaaaac caaaaattca
     1381 ataaaatcaa aaaaggagac cgcagttgga gtcagctgat tcttatatgg tttttaaaga
     1441 cagagttgat gcagcccaaa gacttgctaa aaagcttgaa tggttaaaaa aagaagatcc
     1501 gattattctg gccattccaa gaggtggtgt agttacaggt gatgtcatct cttctgagtt
     1561 aggtgtaaaa ctagatatca tagtttcaag aaaagtggtg gcacctagta acccagagct
     1621 tgcaataggt gcagtgacac acgatggaag ttatttccca aatgtagaca ttacaaggat
     1681 gctaaacatt ccacaaaaat acattgatgc agagatttct gtacaaatga aagagatcga
     1741 acgcagatta atgagattta gaggaagtaa ggaatatcac cttaaaggaa gaactgttgt
     1801 attggttgat gatggaattg ctacaggagc aacaatgttt gtagctatac aatgggttaa
     1861 aaaacaaaaa ccgaaacaac ttattgtagc tgtgccagta ggaccaaaag aaactattga
     1921 taagttaaat gaagtagcag ataaggtagt agtcttacaa tctccagttt attttaatgc
     1981 agttggagaa ttctatcaag aatttaatca ggttgatgat tatcaagtac aagaaattat
     2041 gagtaaacat ggttacaaaa tccagatatt caaataaatg ataatgtatc ttgttttaag
     2101 aaatgtatgc atggcggata tgagcaaaca tgaagcatga tggaaaaaaa atctatgtga
     2161 taaaagcaaa tggacaaagc gttccgtttg acggtaataa ggtcaaagct acttgtatga
     2221 gagcaggagc aagcaataat ctagcagaac gagtcacaca aaaagtacat gaacaattac
     2281 atcatggcat tagaactcga gaaatataca ggatggtctt agctgcgctt gcatctgagg
     2341 atgaaggcag attcattaaa catcgatatc gcctgaaaga atcaatcatg ctgatgggtc
     2401 ctgcgggctt tgcatttgaa atctatgttg gcaagatact tgaaaattat ggatatcatg
     2461 tagaatcaac tcgttcacaa gtaaacggaa aatgtgtaaa acacgaaatt gatctaattg
     2521 ttgactctaa tcttactagt gaaagataca tggttgaatg caaatatcat aatttccctg
     2581 gaatttacac tggcataaaa gaatcattgt acacgcacgc aagattcctt gatctttcag
     2641 aagtgtttga taatgaaatg ttagtgtgca ataccaaggt ttctgatgat gtcataacat
     2701 atgcaaaatg cgtaggtcag aaattattgt gctggaggta tcctgccaaa aaaggattgg
     2761 aaaacatgat tgaagaaaaa ggtttgtatc caataactat tcttgggcta agtgctaaag
     2821 aactactatc actgtctcag tacaaaataa tgcttgctaa agatttgctg accatagata
     2881 tcgatcaact ggctagaaaa atagatatgt cttatactag attacaccga ttacaaaacc
     2941 tggttcaaaa tattctgtat agaaactagc catcaaaacc actgaattta ttcatacaca
     3001 tgtctggatt tgattcacca ttcatgcatg aatatgagac agaacataaa atatggcatc
     3061 tatgatattt ctacacatga taatcaaata tgataattgc attgtcctac taaaagtggg
     3121 aaaatttgta atatgagatc gttttagatg aaactccaag tagacattct gggaatcgaa
     3181 tcaggtggaa aaccaatcgt atttctaaac aaatttgatg cagatgaaat tggtgtcact
     3241 gcatcaggca gagtgaaaat aaaggcaacg aaggaaatca cagcaatagt aa
//
LOCUS       DATIDL010000153         2183 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrosopumilaceae archaeon isolate SMAG_U16342
            k141_2615639, whole genome shotgun sequence.
ACCESSION   DATIDL010000153 DATIDL010000000
VERSION     DATIDL010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935836
            Sequence Read Archive: SRR25158326
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosopumilaceae archaeon (soil metagenome)
  ORGANISM  Nitrosopumilaceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrosopumilales;
            Nitrosopumilaceae.
REFERENCE   1  (bases 1 to 2183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/04/2024 05:09:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,520
            CDSs (total)                 :: 1,474
            Genes (coding)               :: 1,474
            CDSs (with protein)          :: 1,474
            Genes (RNA)                  :: 46
            rRNAs                        :: 1 (5S)
            complete rRNAs               :: 1 (5S)
            tRNAs                        :: 43
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2183
                     /organism="Nitrosopumilaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2615639"
                     /isolate="SMAG_U16342"
                     /isolation_source="soil"
                     /db_xref="taxon:2202732"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            113..328
                     /locus_tag="VLB45_06805"
     CDS             113..328
                     /locus_tag="VLB45_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008301376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L37"
                     /protein_id="HSD05441.1"
                     /translation="MAKSRSSSLKGLGAKYGLKPRKKYTAIHRLLKTKRKCSECGSIK
                     FGREAVGIWSCKKCGFKIAGLAYDVKV"
     gene            312..557
                     /locus_tag="VLB45_06810"
     CDS             312..557
                     /locus_tag="VLB45_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KEOPS complex subunit Pcc1"
                     /protein_id="HSD05442.1"
                     /translation="MTSKFSAIVEIFAQEKTNSIFESLQTDNKFYSENPTNTKMSLDE
                     KITILIETQELSHLRANLNSTLRLVQTSDDSIESVKI"
     gene            581..976
                     /locus_tag="VLB45_06815"
     CDS             581..976
                     /locus_tag="VLB45_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prefoldin subunit beta"
                     /protein_id="HSD05443.1"
                     /translation="MSSGGQQIPPWLQEQVGRLQQLQQNLQSIMGQKQHLEMENLETE
                     RALEVLKKAADTDTVYKSAGTILIKSTKANLIAELEEKKELSNTRVTVLSKQEVRVKE
                     NLKEVEDKIKTMLQGTTGSNPTQKTDTSK"
     gene            1007..1693
                     /locus_tag="VLB45_06820"
     CDS             1007..1693
                     /locus_tag="VLB45_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010192385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ERCC4 domain-containing protein"
                     /protein_id="HSD05444.1"
                     /translation="MNLEELRMIVDERERKSGIPDLLRAVGIKVELTNLPIGDYIVAP
                     ETVVERKSIQDLISSVFDGRLFDQCHRLKEHFEHPAILMEGNVDKIDEITENPLVFYG
                     ALSSIALDFKIPIIPTPSASHSAKLLVSMCARQNNSKGPFLKKIKKYNDIQRQQLSVL
                     CSLPGVGEKLATRMLEKFGSPTNTLKASYNELAKIQGLGESRALKIRKMLDAESKLQK
                     KINQKTLHDV"
     gene            complement(1773..>2183)
                     /locus_tag="VLB45_06825"
     CDS             complement(1773..>2183)
                     /locus_tag="VLB45_06825"
                     /inference="COORDINATES: protein motif:HMM:NF028455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide-binding protein"
                     /protein_id="HSD05445.1"
                     /translation="IKHIKKHHDAVNALTDTNRLEIKDPEIKYVETVSLKAKDVGDFQ
                     NLSQGDLSVISLCLQIHAELISDDFAVSNTAKHLGINVIPVMTKGIARVIEWTYFCPG
                     CEKIFSKASQCPLCGNKLSRKLDKRKSSVNPIRK"
BASE COUNT          803 a          379 c          405 g          596 t
ORIGIN      
        1 ctacaaaaag gaggaagtga cggatttaca gttgaacaac tacacaaatg ttctgaaata
       61 tcgctttctg tgggaacaaa aataaggaag caacttgagc agctgatgta gtatggcaaa
      121 aagcagaagc tcatcattga aaggactcgg ggcaaagtat ggtctaaaac ctcgtaaaaa
      181 atacactgct attcatagac tcttaaaaac aaaaagaaaa tgtagtgaat gtggttctat
      241 aaaatttggc agagaagcag taggaatatg gtcctgcaaa aaatgcggct tcaaaatagc
      301 aggtcttgca tatgacgtca aagtttagtg ccatagtaga aatttttgca caagaaaaaa
      361 ctaactccat atttgagtca cttcaaaccg ataacaaatt ctattcagaa aacccaacaa
      421 atacaaagat gtcacttgat gagaaaatta caatcttaat tgaaacgcaa gaactttctc
      481 acttgagagc aaatctgaat tctactttac gtcttgtaca aacaagtgat gattcaattg
      541 aatcggtaaa aatataagca aaacaaatgc tggatctggc atgtcctcag gcggccagca
      601 aattcctcct tggttacaag aacaagtcgg aagattgcag caactacaac aaaatcttca
      661 atcaataatg ggacaaaaac aacaccttga aatggaaaat ctagaaacag aaagagccct
      721 tgaggtgctc aaaaaggcag ctgatactga tactgtatac aagagtgctg gtactatatt
      781 gattaaatca acaaaggcaa atttgattgc agagcttgaa gagaaaaaag agctttctaa
      841 tacacgggta acagtattat caaaacaaga agttagagtt aaggagaatc tcaaggaagt
      901 ggaagacaag atcaaaacaa tgcttcaagg aactactggt tctaatccga cgcaaaaaac
      961 tgatacttcc aaataaaata ttaacaaact tcacaaacac tctgttatga atctagaaga
     1021 gttacgaatg atagttgacg aaagggaacg taagagtgga atccctgatc ttttgcgcgc
     1081 tgtaggaata aaggtggaac tgacaaatct cccaataggc gattacatag tagcccctga
     1141 aacagttgta gaaagaaaaa gcatccaaga tctcatttca tctgtttttg atggacggtt
     1201 atttgaccag tgccataggc ttaaagaaca ttttgaacat cctgcaattt tgatggaagg
     1261 aaatgttgac aaaattgatg aaattacaga aaatcctctt gttttttatg gtgccctctc
     1321 gtcaattgct ttggatttta aaattccaat aattcctaca cctagtgcgt ctcattctgc
     1381 aaaactgtta gtatcaatgt gtgcaagaca aaacaacagc aaagggccat tcttaaaaaa
     1441 aataaaaaaa tataatgaca tacaaaggca acaactttca gttttgtgta gtttgcctgg
     1501 tgtgggagaa aaattggcta ctcgcatgct tgaaaaattt ggttctccaa ccaatactct
     1561 aaaggcatcc tataatgaac ttgcaaaaat acaaggatta ggagaatcac gagctcttaa
     1621 gataagaaaa atgcttgatg cagaaagcaa actccagaaa aaaataaatc aaaaaacatt
     1681 acatgatgta tgacacacaa tccaaatgtc tcacacagac taaactaatg atgatccctt
     1741 attcccggct tgttgaatat atcaacaatg gctcacttgc ggattggatt gacagaagac
     1801 tttctcttgt ctagtttacg gctaagtttg ttaccgcaaa gggggcattg tgatgccttg
     1861 gaaaatattt tttcacaccc tgggcaaaaa tacgtccatt ctattacacg agcaatgcct
     1921 tttgtcatga ctggaatgac attgatacca agatgtttgg cagtatttga aactgcaaaa
     1981 tcatcactaa tgagttctgc atgtatttga aggcatagag atattactga aagatctcct
     2041 tgggataaat tttgaaagtc tccaacatct tttgctttaa gtgaaacagt ttccacatat
     2101 tttatttcag gatctttaat ctctaatctg ttagtatcag taagagcatt tactgcatca
     2161 tgatgttttt tgatatgttt tat
//
LOCUS       DATIDL010000154         3392 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrosopumilaceae archaeon isolate SMAG_U16342
            k141_1204219, whole genome shotgun sequence.
ACCESSION   DATIDL010000154 DATIDL010000000
VERSION     DATIDL010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935836
            Sequence Read Archive: SRR25158326
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosopumilaceae archaeon (soil metagenome)
  ORGANISM  Nitrosopumilaceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrosopumilales;
            Nitrosopumilaceae.
REFERENCE   1  (bases 1 to 3392)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3392)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/04/2024 05:09:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,520
            CDSs (total)                 :: 1,474
            Genes (coding)               :: 1,474
            CDSs (with protein)          :: 1,474
            Genes (RNA)                  :: 46
            rRNAs                        :: 1 (5S)
            complete rRNAs               :: 1 (5S)
            tRNAs                        :: 43
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3392
                     /organism="Nitrosopumilaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1204219"
                     /isolate="SMAG_U16342"
                     /isolation_source="soil"
                     /db_xref="taxon:2202732"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..626)
                     /locus_tag="VLB45_06830"
     CDS             complement(<1..626)
                     /locus_tag="VLB45_06830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05446.1"
                     /translation="MISKGVAFSVVMLCAVSFIPNSFAIQADPHFYVKGMADQYGPFA
                     GHYVRILADGERGTILLTTTHGLAVVRMNLSDVVMCYNKTLAICLNGMVSSVKNVAGI
                     GIGDIIRLSADTSGKWSMITFLTGDRTLTTVSSKLNPVKNQISDEVSPQRSFSISKQE
                     NNHLTLLSYYADKPMMNAIEKAQQFTVTHPTFMFDGIQSSLNLKLTSV"
     gene            898..1917
                     /locus_tag="VLB45_06835"
     CDS             898..1917
                     /locus_tag="VLB45_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007401863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05447.1"
                     /translation="MKKTLGLAISFLVISIPFQAWAEYDSSHIPDPQIIMTPTSGSPG
                     TKITITISDLPDISKEPFPYPNLYIYLPFVDAVGNNVPGGCNGESCFAVYTFEDAQNK
                     RFESRTVVFTLFGLNNPKSTYLNGKIYSVCDIKLNERIQQSFGETCHTKTIPPGNYEV
                     KLAWGTLSPTEERYNIIKTMNFTVTEDKTESVAVMKPRDLAVQQYKDGVITESEFDAR
                     LNALGYSFTDIRQLKALIGKISYADKTETSLGSQIPLWIRSNAAWWVEGKITDDDFTA
                     GIDFFIEKNIIRISEERKTNAELPRHVPWWVKNNAKWWSEGQITDRDFVLGIEHMVTN
                     KIIIV"
     gene            1965..2876
                     /locus_tag="VLB45_06840"
     CDS             1965..2876
                     /locus_tag="VLB45_06840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05448.1"
                     /translation="MKHQVLMLPIFVILVLSLVPSTFAEESVNLATTTRATLHYSSSY
                     DKMQADADTSITLQSDEHLYNPGDAGIVEGTVSSNVITGLGEALSLITITVVDANGKI
                     IAEEESEISDGEYFAIFTIPNDAAPGQYTVTSKIEADADVLDLIGLDATANLESSAKF
                     MVAAEVNHDISVDNNDFEISIASNSKISNVEFEKEEKKISFMVEGETGTEGVAEITIP
                     TAMLSGTMSVMIDGQIVASDDVIVKSNTEVQTTFEINYHHSVHTIDIVGTQAVPEFPS
                     SILTIMIVGTIAAVFLARTQTLHAPKI"
BASE COUNT         1141 a          639 c          630 g          982 t
ORIGIN      
        1 attacacttg tcaatttaag attcaggctt gattgaattc catcaaacat gaaagtcgga
       61 tgtgttactg taaactgttg tgccttttct attgcattca tcatgggttt gtctgcataa
      121 tagctaagaa gtgttagatg attattttct tgcttagaga tggagaaaga tctttgaggc
      181 gaaacctcat ctgatatctg gtttttgaca ggatttagct tagatgatac cgtagtcaag
      241 gttctatcac ctgtaagaaa tgtgatcata ctccactttc ctgatgtgtc tgcagaaagt
      301 ctgattatgt caccaatgcc aattcctgca acatttttca ctgatgaaac catgccgttt
      361 agacagattg caagtgtctt gttataacac atgacaacat ctgacagatt catcctcaca
      421 actgccaagc catgtgttgt ggtaagcagg attgttcctc gttctccgtc agctaaaatt
      481 cgtacatagt gacccgcaaa agggccgtac tgatctgcca ttcctttgac ataaaaatgt
      541 ggatctgcct gaattgcaaa agaattcgga ataaatgaaa ctgcacacag cattacaaca
      601 gaaaaggcaa ctccttttga tatcactata attacattta caaaatgaat taaaactctc
      661 gcgtagaatt tctatgcaac actttaatat tatcagagct gtttgcaaaa aattagtcta
      721 gttctctaaa ccccattcat cttcttgcag tccccactca tcctcatctt cccactttgt
      781 cacatttgca agatcatctt cgctaatcat tagaacacaa taatgagaca tgcaaataat
      841 cttcgctgat gaattctacc caatttttat taattatcat actaggataa gcttattgtg
      901 aaaaagacac ttggtcttgc aatatcgttt ctagtcatca gtataccatt ccaagcatgg
      961 gcagagtatg attctagtca cataccagat ccccaaatca tcatgactcc aacatctggg
     1021 agccctggta caaaaataac cataacaatt tcagacctgc cagacatatc aaaagaaccc
     1081 tttccctatc ctaatctcta catttacctg ccgtttgttg atgcagtagg caataatgtt
     1141 cctggtggtt gcaacggtga atcatgtttt gctgtttata cttttgaaga tgcgcagaac
     1201 aaaagatttg aatcaaggac tgttgttttt acactttttg gtctgaacaa tccaaaatca
     1261 acctatctga acggaaagat atacagtgtt tgtgacatca aactaaacga aagaattcaa
     1321 caaagctttg gagaaacttg tcataccaag acaattcctc caggaaatta cgaggttaaa
     1381 cttgcttggg gaacactctc gccaaccgag gaaagataca acataatcaa gactatgaat
     1441 ttcacagtaa cggaggacaa aactgaatct gttgccgtga tgaaaccgcg cgaccttgca
     1501 gtacaacaat acaaagatgg agtgataaca gaatcagagt ttgatgcaag actaaatgct
     1561 cttggatatt cttttacaga catcagacaa ctcaaggcat tgattggcaa gatatcttat
     1621 gctgataaaa cagaaacatc gcttggctca cagattccat tatggataag atctaatgcc
     1681 gcatggtggg ttgagggaaa gataacagat gatgacttta cagcaggaat tgattttttt
     1741 atagaaaaaa acatcattag aatttcagag gaaagaaaaa ccaatgcaga actgccaagg
     1801 catgtcccat ggtgggtaaa aaacaatgcc aagtggtggt cagaaggtca gataactgac
     1861 agagattttg tcttgggcat tgagcacatg gtgacaaata agataatcat agtctagtac
     1921 aaatcatgaa caagatttat ttgcattaaa tcccattttt attcatgaaa catcaggttt
     1981 tgatgttacc tatttttgtg attctagtac tttctcttgt gccttctaca tttgctgaag
     2041 aatcagttaa tctagcgaca acaacaagag ctacattgca ctattcatca tcgtatgaca
     2101 agatgcaagc agatgcagac acatctataa ctttgcaatc agatgagcat ctttacaatc
     2161 caggagatgc agggattgtt gaaggaacag tatcgtcaaa tgtaattaca ggacttggag
     2221 aagcactaag ccttatcacc attacagttg ttgatgctaa cggaaagata atagcagaag
     2281 aggaaagcga aattagtgat ggagaatatt ttgcaatatt tacaatcccc aatgatgcag
     2341 cacccgggca atatactgta acttcaaaaa tcgaagcgga tgcagatgtg cttgacttga
     2401 ttggcttgga tgccacagca aacttggaat cttccgctaa attcatggta gcagctgaag
     2461 taaatcatga tatttctgta gataataatg attttgagat atccatcgca agtaattcta
     2521 aaataagcaa cgtagaattt gaaaaagaag agaagaaaat ttcatttatg gtagaaggtg
     2581 agacgggaac ggaaggtgtt gctgaaataa ccattcctac tgcgatgcta agcggtacaa
     2641 tgagtgtcat gatcgatggc cagattgtag catcagatga tgtcatagta aaatcaaata
     2701 ccgaagtaca aaccacattt gagataaatt accaccacag cgtacataca atagacatag
     2761 tgggaacaca ggctgttcca gaatttcctt cttccatact gacaatcatg atagttggga
     2821 caattgcggc agtgtttttg gcaagaacgc aaactttgca tgctccaaag atttaggaat
     2881 caaaaatgca agaaattaag tagtgtcagg tcatctggtt tatcatggca aaaagaaaaa
     2941 caggtaagca tcaaagaaaa gcagatcaac acgcggtacg tagaaaaaga aagtaatttt
     3001 caaatttact cgttttttaa agacgcagta cttcaatgcc gcataaagaa cacttgtcaa
     3061 gaaagaggtt tactaccttc cagatatgct gcccatttcc agatttcgga cattttgctt
     3121 ttgtcttttt caatcagaca agatacatga aaatgcgata tatcagaaga gaagtaagtc
     3181 agaaatacat attgtaaaat ggatctaaaa ttagtataga tcctactatt gcgatcgcta
     3241 gcaatcaaat caactataaa aacttgattt taatttaacg tagatcttcg gtccattata
     3301 tccatcatta gatcagttat gccattaagt gagctgtcaa ttttttccat atgacctaat
     3361 agtgccttgt cttctttttt ccaagccatg ac
//
LOCUS       DATIDL010000155        12203 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrosopumilaceae archaeon isolate SMAG_U16342
            k141_1808699, whole genome shotgun sequence.
ACCESSION   DATIDL010000155 DATIDL010000000
VERSION     DATIDL010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935836
            Sequence Read Archive: SRR25158326
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosopumilaceae archaeon (soil metagenome)
  ORGANISM  Nitrosopumilaceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrosopumilales;
            Nitrosopumilaceae.
REFERENCE   1  (bases 1 to 12203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12203)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/04/2024 05:09:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,520
            CDSs (total)                 :: 1,474
            Genes (coding)               :: 1,474
            CDSs (with protein)          :: 1,474
            Genes (RNA)                  :: 46
            rRNAs                        :: 1 (5S)
            complete rRNAs               :: 1 (5S)
            tRNAs                        :: 43
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12203
                     /organism="Nitrosopumilaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1808699"
                     /isolate="SMAG_U16342"
                     /isolation_source="soil"
                     /db_xref="taxon:2202732"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..250
                     /locus_tag="VLB45_06845"
     CDS             <1..250
                     /locus_tag="VLB45_06845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05449.1"
                     /translation="DENKKSLEQAVTAWKNRNEFTTKIGKGKYVLAAYEKIKRITMPL
                     DDEHFLYITTEVDANHDKIIEKALKLKQDALHAPEGMP"
     gene            complement(271..627)
                     /locus_tag="VLB45_06850"
     CDS             complement(271..627)
                     /locus_tag="VLB45_06850"
                     /inference="COORDINATES: protein motif:HMM:NF042555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6659 family protein"
                     /protein_id="HSD05450.1"
                     /translation="MNYDKLCKDVLETDKAVRFAGIYGKNGELLASAMRENTEALLTP
                     DEVKMSLYHAKLRWDTRKALAHRLGKEKYSMTEYEKIKRVSIPVEENQLLLVSMETNV
                     DHARTIDRICNLISEQ"
     gene            complement(653..1021)
                     /locus_tag="VLB45_06855"
     CDS             complement(653..1021)
                     /locus_tag="VLB45_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6659 family protein"
                     /protein_id="HSD05451.1"
                     /translation="MDFERLYKEIMNLDPNVRLAIVLNRSGERISGGYRENLKHFLTS
                     DELSMVLYHASQRWEGRKHLAHKIGNALYSMTEYEKVKRMSFPIDENHMVLVSTETSV
                     DHTKIINGVLKLIRNNFPKS"
     gene            1169..1948
                     /locus_tag="VLB45_06860"
     CDS             1169..1948
                     /locus_tag="VLB45_06860"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF012630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031012 - extracellular matrix [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HSD05452.1"
                     /translation="MRAMFAVLFAVLLVMAVIPHDDTFAAKSSKFKVSVDELKGPKTI
                     TLPNGQVLERSVHIFYKKDFAKPPNVGGGGKGADTCYAVLAKGANWKVTEPYIVDSTN
                     QAGLADDFVRTATSTSLETWDVQVPAEIFGSEIAGVVDGLDTQTTDGKNEVMFGSIDD
                     PGAIAVTITWGIFGGPPSGRQLVEWDAMFDDADFAWGNADPAAMDFQNIATHEFGHAA
                     GMGHPSNSCTEETMYAFADVGETKKRDLNTGDINGIKALYK"
     gene            2130..2519
                     /locus_tag="VLB45_06865"
     CDS             2130..2519
                     /locus_tag="VLB45_06865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05453.1"
                     /translation="MDLRTVTDRILRIDDAMRYVGFVDNKGNILYAKMNKEKVSLKTH
                     AQEEIFAHDLAIMREMQDLLNQPMGKMTFVFLSRENLHQLIHYFDNFMLYVTLERYLD
                     HDTVLKIADKIQSTTASWLFKTISYNT"
     gene            complement(2542..4449)
                     /locus_tag="VLB45_06870"
     CDS             complement(2542..4449)
                     /locus_tag="VLB45_06870"
                     /inference="COORDINATES: protein motif:HMM:NF016274.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004668 - protein-arginine deiminase
                     activity [Evidence IEA];
                     GO_process: GO:0009446 - putrescine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HSD05454.1"
                     /translation="MEKKITIGLVQTRVSDDIKFNMQNTISKIREAASKGAKIICLQE
                     LYRTKYFPTDEKTDVTKLAEPIPGETTNTLSSLAKELNVIIIAPIFEAASGKNYNTAV
                     VIGSDGNIMGSYRKMHIPQDPFFYEKNYFEEGTSGYQIFKMQNVSFGVLICYDQWFPE
                     AARTLALQGADLIFYPSAIGYLEGDPLSQDDWHYSWENIQRSHAIANGIHVASINRVG
                     TEGNIKFWGASFVCDAFGRLIKRANETEEEVLIAELDLSQNKRIQDGWGFLRNRLPAT
                     YGLLSSPVVNETPKNLGYFMPAEWERHDSTWLAWPHDPVTFPARVKKVEQAYAKMILV
                     LSKNENVNLFVTDLKMKEKVSEILKKKGVDLQKINFYIHDYADVWFRDYGPVFVLNKQ
                     KKQLAFVHWIFNAWGGKYKDLMRDTQIPNVISSRLQLANFKPGLVLEGGSVDVNGKGT
                     MLTTEQCLLDRHRNPHLSKDEIEKYLANYFGTTKTIWLKGGVVGDDTDGHIDNLARFV
                     NPKTVLCAFEENEKDENHEILKQNYDILVNSTDQDGKKLKVVKIPMPGVICSTVRDEK
                     TRLPASYLNFYVANKTVLVPAYGHKNDRVAQKIIAKQFPGRKVVGIDCRDLIYGMGAI
                     HCVMQQQPAII"
     gene            4601..5743
                     /locus_tag="VLB45_06875"
     CDS             4601..5743
                     /locus_tag="VLB45_06875"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HSD05455.1"
                     /translation="MLVTSAYAKGEEVVSATLATQFQLKTQQTAIIESENIQIKFLNV
                     TEDSRCPSDVTCVWEGLVTILVNIKQNGQDLGDFKLTQRGGQEKLASQNFIGQKMDLV
                     EVNPYPKSTEPISFSDYTATLVVSNTDDRTIIIPSDTAKQGCEEKKTCYFPNTMTIPQ
                     NTELTWKNMDSEDHTVTSGNMIDGQDNIFSSGLISPGETFSYKFTTMRSYDYYCMLHP
                     WMIGSINVEFVPFDIEKRIIHEEGLSSDGSILVIVEATEPINTEQLGIHLYFKNSTQS
                     LLTDVNYDLDVIQNNKTIFSLAKQSTHAGIAEHWTEPLQYDSPVDVKVKILGIGLPDD
                     ESNWTGPKGEIIMFSAVPEFSSLTLFIIVAAFAGLFLAKPISKMNL"
     gene            complement(5762..6067)
                     /locus_tag="VLB45_06880"
     CDS             complement(5762..6067)
                     /locus_tag="VLB45_06880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05456.1"
                     /translation="MSVDTSLVHKALVSVVIEQTMLQIGDAPLLNEVLLALYQKHGCY
                     IPDCYDHPEYLLDVFKDLEGSSYLVVADSIRVQLEEYSYHPEIRKFVEKISEIKCQH"
     gene            6250..7416
                     /locus_tag="VLB45_06885"
     CDS             6250..7416
                     /locus_tag="VLB45_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007551172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HSD05457.1"
                     /translation="MNIGIELLGDLAFLLIVCSGVGALAYILRQPLILGFLVAGILIG
                     PFGPFSLIKNVDMLNNFSDVAIVLLLFGVGLSFPLSKLRGVGKVGIVIALVEVLVMLG
                     VGFALGAAFGWSFYDSMFLAAALSISSTAVIVKVLESTGRMETTSALLLTGVLVIEDI
                     VAIIMISTMHSVVVTGAVEFPVIMWEIAKIGMFFAATLTLGILIVPRLFDLISKLERF
                     EITITFALGLAFGLSFLTYNLGFSAATGAFLAGVIIAGSKFSDEISTLITPIREVFVA
                     IFFVTIGALMDLSAIGTFWIPIAVITIVTILAKIFGVYTGVRLFGAGKQYALAMGLSM
                     ASLGEFSFIVLKTGKDLGVVSDMLFPIVGMVVVITTFVGVMLAKAGIKTVEVYE"
     gene            7508..7792
                     /locus_tag="VLB45_06890"
     CDS             7508..7792
                     /locus_tag="VLB45_06890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05458.1"
                     /translation="MSKPAQRNRTGIYFAVAVVTVVVFMMTAVFPVWNLIPREVTETV
                     KIVSVDDSGCVAETPDKFLIKIGPCTSQPGESITVTYDEKIKERARAFLP"
     gene            7874..8278
                     /locus_tag="VLB45_06895"
     CDS             7874..8278
                     /locus_tag="VLB45_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HSD05459.1"
                     /translation="MAEEKTSSFSYTVKTQKTINQAVEDVTASLKEVGFGVLGTLNFK
                     DILQKKGLDYKDEYRLLEVCNPAAAKQVLESNPEIGLLLPCTVAVYQKNGENWISLAR
                     PTSLLANIHDTRLDKFGKEIEQKLVQAIQKVK"
     gene            8284..8808
                     /locus_tag="VLB45_06900"
     CDS             8284..8808
                     /locus_tag="VLB45_06900"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HSD05460.1"
                     /translation="MNEKQFFELLSKRRSIRMFKETAIEESKLDQILEACNLAPSSGG
                     LQTFEIYQIKNNKIKQQLVVAAKDQTFIAESPLVLVFCANPLRSVERFGERSQLFSVQ
                     DATIAAAYAQLTAHVLGLSAVWVGAFDELQVSEVLHLPDGHRPIAILPIGYKNEKPKE
                     KTTRGTKDLLHVIN"
     gene            8877..9614
                     /locus_tag="VLB45_06905"
     CDS             8877..9614
                     /locus_tag="VLB45_06905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05461.1"
                     /translation="MTKLIIVAMLTLVLFIPTSLAFAASGGTPSGQPFQELDQRISAL
                     EESDSEADSFFDVFFDVFTDVDSFFDVFTELQESIANLQSNIDAEAAARAAADTTLQS
                     NIDAEAAARAAADSTLQSRIFQNCPTGQSIRQINVDGTVVCEFDDIGAGGGVGRLIVR
                     EVRDLTPGILPGGGAITDSFAACDTGNGEIPVGGGYAATSTLVNVWREHTAFTAEGFG
                     WLVTASLPTGITPHEITAIVYCAKVVP"
     gene            9710..10261
                     /locus_tag="VLB45_06910"
     CDS             9710..10261
                     /locus_tag="VLB45_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007549804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05462.1"
                     /translation="MKRTSSWGNRFILSAIIQGAIITGLTISVVGAQMISSNVNIIQF
                     LSLSFDGPAKWFFLGYVFYMILVVAIATTAIFYNHLEINMEKNIRGIRSVMAWIHLIG
                     MNVGGAATTISMILAGLMGSGALDLILSAGSTELTPNLAIMDQFIPPIATFAGVLSIG
                     VIVGGISYISTYLQKNESITKDD"
     gene            10315..10788
                     /locus_tag="VLB45_06915"
     CDS             10315..10788
                     /locus_tag="VLB45_06915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05463.1"
                     /translation="MIHPWLDKLLALIAHKMRAGIMAIGVALLVVGIIGYLNTDKIIS
                     ECESEASKAGLFTSEYRQVCDKLPLLNYLMPITVIIGIGLMGYGIVAKKKIKVEKPIV
                     EKPVNSITDDYVKLAKRFEEMSEKYDILEKKHVQLTTYMKRNGIAIPDHIMAKID"
     gene            10937..11722
                     /locus_tag="VLB45_06920"
     CDS             10937..11722
                     /locus_tag="VLB45_06920"
                     /inference="COORDINATES: protein motif:HMM:TIGR03344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HSD05464.1"
                     /translation="MTSKTIFAALAIAFVAGTLSVSTINFAQGAPGSDPYSQIVTAIN
                     NLTDAIQNKETSVTVNAPPGPEGPPGETGPPGETGEQGPPGETGPPGPAAFGTEPPIM
                     KYVKIEGAVQGNIDGGATKNGEEKSIEVISFDHSIISPRDAASGLPTGKRQHSPITIV
                     KEIDKSTPLIYQALVNNENLRSVEFHFYNTDGTGKEVHYFTIKLTNANVASIKQINQN
                     SDARPDLQNFGEYEEVAFTYQKIEWTWVDGGITSMDDWESPVV"
     gene            complement(11775..>12203)
                     /locus_tag="VLB45_06925"
     CDS             complement(11775..>12203)
                     /locus_tag="VLB45_06925"
                     /inference="COORDINATES: protein motif:HMM:NF025774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MEDS domain-containing protein"
                     /protein_id="HSD05465.1"
                     /translation="LTHEDPKIIKDEMNENEINVKEFVQKNLLQIQQITSPEKDPKGI
                     LQGYDNIVKQAIADKKPPYRIVGKIMPNTEIEEAMSVQLVIERMAHAMFEKLNSTILC
                     TYDINEIPTNNYKMWLSRLYSYHHAAIVSIRDGTNMAGYL"
BASE COUNT         3736 a         2305 c         2419 g         3743 t
ORIGIN      
        1 agatgaaaac aaaaaatcgc ttgagcaggc tgtaactgca tggaagaatc gcaacgagtt
       61 tacaacaaag attggcaagg gaaaatacgt cttggctgct tatgaaaaga tcaaacgaat
      121 caccatgccg ctagatgacg aacattttct ttacattact accgaagtag atgccaacca
      181 tgataaaata attgaaaagg ccttgaaact aaaacaggat gcattgcatg cgcctgaagg
      241 aatgccgtaa accgtttctg taaattccaa ttattgctca gatatcagat tgcaaattct
      301 atctattgtc ctagcatgat caacattcgt ttccatgctg actagtagca gctgattttc
      361 ctcaactgga atgctgacac gttttatttt ttcatactct gtcatggaat atttttcctt
      421 gcctagtctg tgtgcaagtg ccttgcgcgt atcccacctt agtttggcat ggtatagtga
      481 catctttacc tcatcaggag tcagcagggc ttcggtgttt tcacgcatgg cactagctaa
      541 aagctcacca tttttaccat aaattccagc aaaacgcact gccttgtctg tttctaaaac
      601 atccttgcat aatttatcat aattcacgaa tctcgttcaa gtagttttaa tattaacttt
      661 taggaaaatt atttctaatt aattttaaca caccattaat tatcttggtg tgatctacac
      721 tggtttcagt acttactagc accatgtgat tttcatctat tggaaacgac attctcttaa
      781 ctttttcata ctctgtcatg gaatacaatg cgttaccaat cttgtgtgcc aggtgttttc
      841 ttccttccca acgttggctt gcatgataaa gaaccatgct aagctcatca gaagttagaa
      901 aatgctttag gttttctcta taaccgccag aaattctttc tccacttcga tttaaaacta
      961 tggctaacct aacgttagga tcgagattca ttatttcttt atacaatctt tcaaaatcca
     1021 tagataaaac accacttttt attattaaaa gacgatcgta taaggacgag tttgatgcaa
     1081 tgagaccgtg tagtttatga tgagatctga aagtacgtat cttttgtatg caatgctttt
     1141 atagaattac cgtaatcatg taatgtaaat gcgtgccatg tttgcagtat tatttgcagt
     1201 gcttttggta atggcagtta ttccgcatga tgatacattt gcagctaaat catcaaaatt
     1261 caaagtctca gttgatgagc taaaaggccc aaaaactatc acgcttccta atggccaagt
     1321 ccttgagaga agtgttcaca ttttctacaa aaaagatttt gctaaaccac caaatgtagg
     1381 gggtggtgga aaaggtgctg atacatgtta tgcagttctt gcaaagggtg ctaattggaa
     1441 agtaacagaa ccatacatag ttgattcaac aaaccaggct ggacttgcag atgattttgt
     1501 taggacagca acatctacta gtttggaaac atgggacgtg caggttcctg ctgaaatatt
     1561 tggctctgaa attgcaggtg tcgttgatgg tcttgataca caaaccactg atggcaaaaa
     1621 cgaagtgatg tttggaagca ttgatgaccc aggtgcaatt gcagtcacaa taacatgggg
     1681 aatctttggt ggaccgccat ctggaagaca gctagtagaa tgggatgcaa tgtttgatga
     1741 tgcagacttt gcatggggca acgcagatcc tgcagcaatg gactttcaaa acatagcaac
     1801 acacgagttt ggccatgctg ctggcatggg acacccgtct aatagctgca ccgaagagac
     1861 aatgtatgca tttgctgacg tgggagaaac aaaaaagcgt gatcttaata ccggcgacat
     1921 taatggcata aaagcactgt acaagtaagt agaatttttt acaactaatt tcaaacacat
     1981 ttttgcaata gtattaaagt attgcataga aattctacgt atgttttatt tatttttcat
     2041 cttctaaaga agcattgaaa caaaaagcgg acgttattca ttttttttac agacatgtag
     2101 ttaaacaatt ttcaggtgta tcataaataa tggatcttcg aaccgttaca gatagaatcc
     2161 tgcgaattga tgacgcgatg cgttatgtcg gctttgttga taacaagggc aacattttgt
     2221 atgctaaaat gaataaagaa aaagttagtc tgaaaactca tgctcaggag gaaatctttg
     2281 cccatgattt ggcaatcatg cgtgagatgc aagacctgtt aaaccaaccg atgggaaaga
     2341 tgacttttgt gtttctatca agggaaaacc tgcatcagct aattcattat tttgataatt
     2401 ttatgctgta tgtcacactt gagagatatc tagatcatga cacggtacta aagatcgctg
     2461 acaaaatcca gtcaactaca gcttcatggc ttttcaaaac catatcatat aacacatagg
     2521 tgttttacaa aaattttctt actaaattat ggcaggctgt tgttgcatga cacaatggat
     2581 tgcacccatt ccgtaaatca aatcacggca gtcaatacca acaacttttc ttccaggaaa
     2641 ttgttttgcg ataatttttt gtgcgacacg gtcatttttg tgcccgtatg ctggaactaa
     2701 gacagtcttg tttgcaacat aaaagttcag atagcttgca ggaagccttg tcttctcgtc
     2761 tctcactgtg ctacaaatca cacccggcat tggaatcttg actactttga gcttctttcc
     2821 gtcttggtct gttgagttta ccaaaatgtc atagttttgt ttcagtattt catgattctc
     2881 gtctttttcg ttttcttcaa aagcacacaa aacagtcttt ggatttacaa aacgagccag
     2941 attgtcaata tgcccatctg tgtcatcacc tacaacgcca ccttttagcc agattgtctt
     3001 tgtagtgcca aaataatttg ccagatattt ttcaatctca tcttttgaga gatgtgggtt
     3061 tctatgtcta tcaagaaggc actgctctgt ggttagcatg gttcctttgc cgtttacatc
     3121 gacagagcca ccttccagga caagacctgg cttgaaattg gcaagctgca atctgctgct
     3181 tattacattt ggaatctgcg tgtcgcgcat caagtccttg tattttcctc cccatgcgtt
     3241 aaagatccag tgcacaaaag caagctgctt tttttgcttg tttaatacaa aaacaggacc
     3301 ataatctcta aaccagacat cagcataatc atgtatgtag aaattaattt tttgcaagtc
     3361 gactcctttt ttctttaaaa tctctgacac cttttctttc atcttcaaat cagttacaaa
     3421 caagttgacg ttttcatttt tggataaaac taaaatcatt tttgcatacg cctgttctac
     3481 ttttttcact cgtgctggaa aagtcacagg atcatgtggc catgcaagcc atgtcgagtc
     3541 atgtctttcc cattctgctg gcataaaata tcccaagttt tttggtgttt catttacgac
     3601 aggtgagcta agtagcccat aagtagcagg cagcctgttt cgcaaaaatc cccagccatc
     3661 ctgaattctc ttgttttggg acaagtccag ttctgcaatc aagacctctt cttctgtctc
     3721 gtttgcacgt ttgatcaatc ttccaaacgc atcgcacaca aacgaggccc cccagaattt
     3781 tatgttacct tctgttccaa ctctgtttat tgatgcaaca tgtattccgt ttgcaatggc
     3841 atgggagcgc tggatgtttt cccaagaata gtgccagtcg tcttgcgaca agggatcacc
     3901 ttccaagtat cctattgcag aaggataaaa gatgaggtct gctccctgca gtgccaaagt
     3961 tcgcgcagct tcaggaaacc attggtcata gcaaatcaaa actccaaatg aaacattttg
     4021 cattttaaag atctggtatc ctgatgtgcc ttcctcaaaa tagttttttt cataaaagaa
     4081 aggatcttgt ggtatgtgca tctttctgta gcttcccatg atatttccat cagatccaat
     4141 cactactgca gtgttgtagt tcttgcccga ggctgcttca aaaattggtg caattataat
     4201 tacgttcaat tcttttgcca aagaagaaag tgtgttagtt gtttctccag gtattggttc
     4261 tgccagcttg gtaacgtcgg ttttttcatc ggttggaaag tattttgtcc gatataattc
     4321 ttgtaagcaa attatctttg cacccttgct tgcagcttct cttatttttg atatggtatt
     4381 ttgcatgttg aatttgatgt catcagaaac tctagtctga acaagtccta ttgtgatttt
     4441 tttctccaac agggcacctt gattcaagat gtttatttat ctttaaccaa agaaagatgg
     4501 ttcgtataat ttatgagcgt acttcataag caagaaaaac caacttatct ttagcttgaa
     4561 catctttggg ctagctatcc ttgtttgctt tgtttgcaca ttgcttgtca caagtgcata
     4621 tgcaaaaggc gaagaagttg ttagtgccac acttgcaaca caatttcaat taaagacgca
     4681 acaaacagcc atcatagaat cagaaaacat acagatcaaa tttctaaatg ttacagagga
     4741 ttctagatgt ccttctgatg ttacttgcgt gtgggaagga ctagtaacca ttctagttaa
     4801 catcaaacaa aatggtcagg atttaggtga tttcaagctt acacaaaggg gaggacaaga
     4861 aaagctggca tctcaaaact ttattggcca aaaaatggat cttgttgaag taaaccccta
     4921 tcctaaaagc actgaaccaa tttcattttc agattatact gctacactag ttgtttctaa
     4981 cactgatgat aggaccatca taattccatc tgacacagca aaacaaggct gcgaagaaaa
     5041 aaagacgtgc tattttccaa atactatgac aattccacaa aatactgaat tgacgtggaa
     5101 aaacatggat tctgaagacc acactgttac aagtggaaat atgatagacg ggcaagataa
     5161 tatctttagc agcgggttga tctctccggg agaaacattt tcttacaaat tcacaacaat
     5221 gagatcatat gattactatt gcatgcttca tccgtggatg ataggctcga ttaatgtaga
     5281 atttgtaccc tttgacatag aaaaaagaat aatccatgag gaagggctgt cttctgatgg
     5341 ctctattctg gttatcgttg aagccacaga gccaattaac acagaacagc ttggcatcca
     5401 tctttacttc aaaaactcta cgcaaagctt gcttactgat gtaaattatg atcttgatgt
     5461 catacaaaac aacaagacaa ttttttcact tgcaaaacaa tcaacccatg caggaatcgc
     5521 agaacactgg acagaacctc ttcagtatga ttcccctgtg gatgttaaag tgaaaatctt
     5581 gggcattgga ttgcctgatg acgaatctaa ctggactggc cccaagggag agattataat
     5641 gttttctgct gttccagagt tttcttcgtt aactttgttt attatagtag ctgccttcgc
     5701 aggactcttc ttggcaaaac cgatatcaaa gatgaatctc tagggtacta aattctctag
     5761 tctaatgctg gcattttatt tcacttatct tctcaacaaa ttttcgtatc tctgggtgat
     5821 agctgtattc ttcaagctgt actcttatag aatctgcaac aacaaggtaa gaactcccct
     5881 caagatcctt gaaaacatct agtaaatatt ctggatgatc ataacagtct ggaatatagc
     5941 aaccatgttt ttggtataga gccaagagta cttcattgag tagtggggcg tctcctattt
     6001 gcagcatagt ctgttctatc actacactta ccagagcctt atgaacaaga ctagtatcaa
     6061 cagacattgc ctatggttag ttggcattgg caataagcag atgtcattct ctgatagaac
     6121 atactgtttt agctctgaac tgttcttgcg caaactagcc aaaagtaagc agaatgcacg
     6181 taacagcaaa ggaatcttga catctcagaa catagcccaa tgatgagaca ccattgttgg
     6241 taattagact tgaatattgg aattgaactg ctaggggact tggcattctt gctaatcgtg
     6301 tgttctggag taggagcttt ggcatacata ctcagacaac cgttgattct tggttttctt
     6361 gttgcaggca ttttgattgg tccgtttgga ccgtttagtc ttatcaaaaa cgtcgacatg
     6421 ctaaacaact tttcagacgt tgcaattgtt ttgttattgt ttggtgttgg actgtcgttt
     6481 ccactatcaa agttgcgcgg tgtaggaaaa gtaggaatcg taattgcact agtagaggta
     6541 cttgtgatgc ttggcgttgg ttttgcatta ggtgcagcat ttggatggag cttttacgat
     6601 tcgatgtttt tggcagcagc attatcaata agctctacag cagtaattgt caaagtactg
     6661 gaaagcacag gaagaatgga aaccacttca gcattgctgc ttacaggagt gctggtaatt
     6721 gaggacattg ttgcaataat aatgattagc acaatgcatt cagtagtggt tactggtgct
     6781 gtcgaatttc cagtaataat gtgggaaatt gcaaaaattg gcatgttttt tgcagccacc
     6841 cttacacttg gaattttaat cgttccaaga ctgtttgatc ttatttcaaa actggaacga
     6901 tttgaaatca ccattacatt tgcgttgggg ctggcctttg ggctatcatt tttgacatac
     6961 aaccttggat tctcagctgc aacaggtgca tttttggcag gagtgataat tgcaggctcc
     7021 aaattctctg atgaaatatc aactttgatc acaccaatca gggaagtctt tgttgcaata
     7081 ttttttgtaa caattggagc tcttatggac ctatcagcaa ttggaacatt ttggattcca
     7141 attgcagtaa ttaccattgt aacaatactt gcaaaaatat ttggagtcta tactggtgtg
     7201 aggttgtttg gtgccggtaa acaatacgcg cttgcaatgg gtctgagcat ggcatcgctt
     7261 ggagaatttt cgtttattgt actaaaaaca ggaaaggatc ttggtgtggt aagtgacatg
     7321 ttgtttccga tagttggcat ggttgtggta atcaccacat ttgttggtgt gatgctggca
     7381 aaagcaggaa taaagactgt cgaagtatac gaataaacag tggatcagat ttgcgactat
     7441 catacctgaa aatcacgttt gacaatcttg attctgtatt atattggatg aattgaccta
     7501 aacaaacatg agtaagccag ctcaaagaaa ccgaacggga atctactttg ccgtagctgt
     7561 agtaacagtc gtagttttta tgatgacggc tgtatttcct gtttggaatc tgattccaag
     7621 agaggttaca gagactgtca aaattgtttc tgttgatgat tcaggttgtg tagcagagac
     7681 tccagataaa tttttgataa aaattggtcc atgtacttct caaccaggag aatcaataac
     7741 agtcacgtat gacgaaaaaa tcaaggaaag ggctagagca ttcttgcctt gatcatcaaa
     7801 ttctttttct aactgattct caagtgtgat atttttcgtc tctattataa tgaatcatat
     7861 tcattactag tgtatggctg aagaaaaaac atccagtttt agctatactg tgaaaacaca
     7921 aaagacaata aaccaagcag ttgaagatgt cactgcaagc ctcaaggaag tgggctttgg
     7981 agttttagga actctgaatt ttaaggacat attgcaaaaa aagggattag actacaagga
     8041 tgaatacaga ttattagaag tatgtaatcc cgccgcagca aaacaagtcc tagaatcaaa
     8101 tcctgagatt ggtttgctat tgccatgcac tgttgcagtg tatcagaaaa acggtgagaa
     8161 ctggattagc ttggcaagac ctacgtcgct tcttgcaaac atacacgata ctagattaga
     8221 taaatttggt aaagaaatag aacaaaagct tgtccaagcc atacaaaaag taaagtgaga
     8281 gtaatgaatg aaaaacaatt ctttgaattg ctaagtaaga gacgctcaat taggatgttt
     8341 aaagaaactg ctattgaaga atctaaacta gatcaaattc ttgaggcatg caatttggct
     8401 ccgtcatctg gtggtttgca gacctttgaa atttatcaga tcaaaaataa caaaataaaa
     8461 caacaattag ttgtggcagc aaaagaccag actttcatcg cagaatctcc gttggtgcta
     8521 gtgttttgtg caaacccatt gcgttctgta gaacgctttg gagaaagatc tcaattattt
     8581 tcagtccaag acgccacaat tgcagctgca tatgctcagc tgacagctca cgtattggga
     8641 ttatctgctg tttgggtagg tgcatttgat gagttgcaag tctcagaagt cttgcatctt
     8701 cctgatggtc atagaccaat agcaatactt ccaattggtt ataaaaatga aaaaccaaag
     8761 gaaaagacga caagaggaac caaagaccta cttcatgtca taaactagtc tgagagatat
     8821 tttctataat atctattggc ataatgctta actaaaacta attttatgat cggacaatga
     8881 caaaattaat catcgtagca atgctgacac tagtgctttt cattccgacc tcactagcgt
     8941 ttgcagcttc aggtggaact ccaagtggac aaccatttca ggagctagac cagcgaataa
     9001 gtgcattaga ggaaagtgat tcagaagctg actcattttt tgacgtcttc tttgatgtct
     9061 ttacagacgt ggatagcttc tttgacgtct tcacagaact gcaagaatct attgctaacc
     9121 tgcaatcaaa cattgacgca gaagctgctg cacgagcagc tgcagacaca actctgcaat
     9181 caaacattga cgcagaagct gctgcacgag cagctgcaga ttcaacactg caaagcagga
     9241 tttttcaaaa ctgtcctact ggtcaatcaa ttagacagat caatgtagat ggcactgtgg
     9301 tgtgtgaatt tgacgacatt ggtgctggcg gcggtgttgg cagattgata gttagagaag
     9361 taagagatct aactcctggc attttgcctg gcggtggagc aattacagac tcgtttgcag
     9421 cttgtgacac tggtaatggt gaaattccag tcggtggtgg ttatgcggct acaagtacat
     9481 tagtaaatgt atggcgagag cacactgcat ttacagcaga aggatttggt tggcttgtaa
     9541 ctgcttctct tcccacagga attacaccac atgaaatcac agcgattgta tactgtgcaa
     9601 aagtagtccc atagacttct aaccattctt tttttatttt taattccctg ctctagtagc
     9661 aacactatct tttcaattag tttatccata tgtgaaatat agtacagaca tgaagcgcac
     9721 aagctcttgg ggcaacaggt tcattttatc tgcaataatt cagggtgcaa taattacagg
     9781 actcaccatt tctgttgtgg gggctcagat gatctcctca aatgtaaaca taatccagtt
     9841 tctttctctg tcttttgacg gacctgcaaa atggttcttt ttgggctatg tattttacat
     9901 gatacttgtc gtggcaattg caactacggc aatattttac aaccatttgg aaatcaacat
     9961 ggaaaaaaac atccgtggaa tcaggtctgt aatggcttgg attcatctga taggaatgaa
    10021 tgtcggaggt gcggcaacta ccatatcgat gatacttgcg ggactgatgg gttcaggtgc
    10081 acttgattta attttgagtg caggaagtac cgagctaact ccaaaccttg ccatcatgga
    10141 ccaattcatc ccaccaattg ctacatttgc aggagtgtta agcattggag taattgttgg
    10201 tggaatatct tacatctcaa cttatctgca aaaaaatgag tcaataacaa aagacgattg
    10261 aaaaataaaa aataactgat tttctacact ttcaaaagta attgtcagta cgtgatgata
    10321 catccatggc ttgataagct cttggctcta attgcccaca agatgagagc tggtattatg
    10381 gcaatagggg ttgcacttct agtggtggga ataattggtt atctaaacac ggacaaaata
    10441 atatcggaat gtgagtctga ggcaagtaag gctggccttt ttacaagcga gtatagacaa
    10501 gtgtgtgata agcttccatt gttaaactat ctaatgccca ttacggttat cattggaata
    10561 ggactcatgg gatatggtat agttgcaaaa aagaagatca aagtagaaaa accgatagta
    10621 gaaaaacctg tcaattctat taccgatgat tatgtcaagt tggcaaaaag atttgaagag
    10681 atgtcggaaa aatatgatat tctggaaaaa aaacatgttc aactcacaac atacatgaaa
    10741 agaaatggca tcgcgatacc agatcacatc atggcaaaaa tagattgata aatttgggtt
    10801 tcagaaatat tgttttgatc aacaaattcc atctttgatt agaaattata acaaaattta
    10861 ggaaaatact tggtataatt ttaggccatt taccacaaaa ttactcttta aatatctgta
    10921 aaatttgatt gaattaatga ctagtaagac aatctttgct gcattagcaa ttgcttttgt
    10981 ggcagggaca ctaagtgttt caacaatcaa ctttgcacaa ggcgcgccag gatcagatcc
    11041 atatagccag attgtaacag caataaacaa tcttacagat gcaatacaaa acaaagagac
    11101 atcagtcact gtaaatgctc ctcctggacc agaaggtcca ccaggtgaaa caggtccgcc
    11161 aggtgaaacg ggcgaacaag gtccaccggg tgaaacagga ccaccaggac cagctgcgtt
    11221 tggaacagaa ccaccaataa tgaaatatgt gaaaatagaa ggtgcggtac aaggaaacat
    11281 tgatggcggc gcaacaaaaa acggtgaaga aaaatctatc gaagtaataa gctttgatca
    11341 cagcataatc tcaccacgtg atgctgcatc tggacttcca actggaaaaa gacaacacag
    11401 cccaattaca atagtgaaag aaattgataa atcaactcca ctgatttacc aggcacttgt
    11461 aaacaacgag aatctaaggt cagttgaatt tcatttctac aacacagatg gtacaggcaa
    11521 agaggtgcac tactttacaa taaagctaac caatgcaaac gttgcatcaa tcaaacagat
    11581 aaaccaaaac tctgacgcac gtcctgactt gcagaatttt ggggaatatg aagaagttgc
    11641 tttcacatat caaaagattg aatggacatg ggttgatggt ggtatcactt caatggatga
    11701 ctgggaatct ccagttgtct aaattcactt ttttatcatt acaattatct tgctagtgtc
    11761 tgactagttt aaatctacaa atagcccgcc atgtttgttc catctctaat agatacaatt
    11821 gcggcatggt ggtatgaata cagacggcta agccacatct tgtaattatt ggtcggaatt
    11881 tcattaatgt catatgtaca aagtatagtt gaattaagtt tttcaaacat tgcatgagcc
    11941 atgcgttcta ttacaagctg aactgacatt gcctcttcta tctctgtgtt gggcattatt
    12001 ttgcccacaa tcctgtatgg cggcttttta tcggcaatgg cttgttttac aatattatca
    12061 taaccttgca gtattccttt aggatctttt tctggactgg tgatttgttg tatctgcaac
    12121 aggttttttt gtacaaactc ttttacgttt atttcatttt cattcatttc gtctttaatt
    12181 attttggggt cttcgtgtgt gag
//
LOCUS       DATIDL010000156        48785 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrosopumilaceae archaeon isolate SMAG_U16342
            k141_2417785, whole genome shotgun sequence.
ACCESSION   DATIDL010000156 DATIDL010000000
VERSION     DATIDL010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935836
            Sequence Read Archive: SRR25158326
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosopumilaceae archaeon (soil metagenome)
  ORGANISM  Nitrosopumilaceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrosopumilales;
            Nitrosopumilaceae.
REFERENCE   1  (bases 1 to 48785)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48785)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/04/2024 05:09:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,520
            CDSs (total)                 :: 1,474
            Genes (coding)               :: 1,474
            CDSs (with protein)          :: 1,474
            Genes (RNA)                  :: 46
            rRNAs                        :: 1 (5S)
            complete rRNAs               :: 1 (5S)
            tRNAs                        :: 43
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48785
                     /organism="Nitrosopumilaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2417785"
                     /isolate="SMAG_U16342"
                     /isolation_source="soil"
                     /db_xref="taxon:2202732"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..426
                     /locus_tag="VLB45_06930"
     CDS             <1..426
                     /locus_tag="VLB45_06930"
                     /inference="COORDINATES: protein motif:HMM:NF020014.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HSD05466.1"
                     /translation="LLDNVIVGRITNTAEQVKYLEKTLEIKDLYLVVIDNITDLFSFE
                     YSKESKSLTKHIEFMKYMHNLSSVAIKKKIAIVVTNMVRKSDEKERENLDRSISMSVH
                     QKIHLVKIGHKYVAEVRPSFGIKKEIPYMITPSGLVDSS"
     gene            588..3287
                     /locus_tag="VLB45_06935"
     CDS             588..3287
                     /locus_tag="VLB45_06935"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007549384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HSD05467.1"
                     /translation="MTKFHSLGFENLTDIQKKAVPIIFQKIDSLVVAPTGSGKTESSV
                     IPIFARIAKSKKPGKIKALYITPLRALNRDVFRRIMKYAEQEALSIQIRHGDTSQSIR
                     KKISDSPPDILITTPETIVILLTQQKMLHALSELEWVVIDEVHELLGNERGAQLSLSL
                     ERLQNNSKSDITRIGLSATVGNIEECAKFVVGTKRKCKVIRDTSIRKYDVEIKYIDGT
                     ITDVVDFIIEYVAKNYQNSPVLLFTNTRGESEYIASILREKSTSRIELHHGSLSQEVR
                     EETEENLRQGKPGIIVCTSSLELGLDIGSVELVIHYGSPRQVSKLVQRIGRSRHNRDS
                     SAKGLVITNNPDDEFEARAILERVKEKSIEDQIIHADSLDVLAHHLVGLTMQFGSVPV
                     DLAFKIIRQAYPFRNTTLEDLFGVLDLLHSSSVIFFDKEEMLFKKKGRAFRYYFENLS
                     TIPDILKFKVFDTASKKIIGSLDQRFVGDYGDPGNVFVLRGMQWRILNVDESSLKINV
                     EPIQSAGINVPYWEGENIPVDYATASKVGSFRSKVQMKSMVVLNKVIENLKLSEIPNE
                     KSVVVESLRSRNMVVIHSCFGTKINSTISMLLSTMLSAKTGHLVDTRSDAYRIVLSST
                     GRIPEKQVLDILNEEYDLPLVVTASLTGTHNINWRTWCVAKKFGIVGRGAIYDRKSAR
                     FIYEKYSKTSLAKEALRELFHDKYDLKNTQTLLEKIKAKEILLKWIEVDSFSKLAESI
                     LDHTTKYYASPANIDKGILDLVKTRLLKTKHRLICARCGKWEKVVETKEVNDVLSCHY
                     CKSRQITATFYSDYDLGKIIQKKHSGKKISTEENHKFSRAWKISSLLENFGKTALIVL
                     SGYGVGADTGARILRNMIGEEELYKQIYEAERQYVTTRGFWDS"
     gene            complement(3284..4681)
                     /locus_tag="VLB45_06940"
     CDS             complement(3284..4681)
                     /locus_tag="VLB45_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HSD05468.1"
                     /translation="MSEFELLLNKLLEQKPDLSRSDIQEMINKKKEKIGAGYLTDQGA
                     LFLIASDLGVSLNEQLKVEMGLKDLYVGAKEITLETRVMNVYPAKQFSRKDGTQFLLR
                     TITVYDSDSRAKVKLWDEKANLPGIESLKPGDLVKIIKAYVKSDMTGTPIINIGSGSN
                     LETSGMKSSIPLLDSITEDVSMVKEDQQNLVVSGMLDGNVRMSEFTNFKGQPGKALHL
                     RLKGKDGTIIRVVLWNTDENSIPKMITSGAKTRLIGVKTKRGQQGIELHGDEGTVIEI
                     EGANEIQPLTVRVLSISKNDYGNNLILVVDQKKKLLNITDFANVSKDMQVGEVIECMP
                     TKAYGNSITLEKDALIRKINDENIPTISDIRTKIRDIKPSDNTYCVEAIILKMPERRE
                     IQTKTGESVALSEMFIEDETGQIWLKGWRNQARMLDQFSQGEIVSIIGVTAKAGLEGR
                     IELFLTPFSQITKKN"
     gene            4757..5698
                     /locus_tag="VLB45_06945"
     CDS             4757..5698
                     /locus_tag="VLB45_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007403173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSD05469.1"
                     /translation="MSCIETNLLSKSYDSTIAVDRIDLSVYSGKIFGFLGPNGAGKTT
                     TIKLLTTLIAPTSGSIDILGIDAIRHPLDVRKRIGVVLQQPSYEPTLSVEKSLEKYGM
                     MWNVERKTRKDRVEELLLSFALEDIRNVKNEDLSIGQRRRVQVAREFMHDMELLFLDE
                     PTVGLDPSARRTLLDFIKKRVKAGLTVFFTTHILHEAEYLCDEIAIINKGKIIAIDTP
                     EDLKNKFGREKTIKIHINNTEKNMSSLLEGIQDYKLDFNSGTNITIRSNNSEEILLKI
                     LQILTANNITIDDLSAVPTSLEEIFLSMVKKSNASNN"
     gene            5682..6443
                     /locus_tag="VLB45_06950"
     CDS             5682..6443
                     /locus_tag="VLB45_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007403172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSD05470.1"
                     /translation="MHPIIRLVNRNLTISLNKGFLIWQVIFPLIYIFVGGFAYTSLID
                     SVPFANKTLDYPAYIASGMIGFNIMNSTLVSGIIIWNDRRHGMFEQILVGPFTRRHYI
                     LSNVYTIGIVGLISAALITIVGYPLFFNSVEFNLFTIPLLVFASVAGSILFGSIASII
                     STRLRSSEGFNVVINTVFLFFAFVSTAFYPAEGAPAPIATAFYFNPLTYLVDVVRAGI
                     FGSFDTFVALEMLVLVILSLGLFAVATKLLAKLEL"
     gene            6494..6730
                     /locus_tag="VLB45_06955"
     CDS             6494..6730
                     /locus_tag="VLB45_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007550438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05471.1"
                     /translation="MEVRKRSSFWFLLPIFFNVIGGVIAYFIIKDDDPRKAKNCLLLG
                     IILTAISFAIFVVPILIGISLMPNFDMMPNEPYL"
     gene            complement(6743..6830)
                     /locus_tag="VLB45_06960"
     tRNA            complement(join(6743..6805,6821..6830))
                     /locus_tag="VLB45_06960"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6779..6781),aa:Gln,seq:ttg)
     gene            complement(6934..7965)
                     /locus_tag="VLB45_06965"
     CDS             complement(6934..7965)
                     /locus_tag="VLB45_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007549389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF354 domain-containing protein"
                     /protein_id="HSD05472.1"
                     /translation="MKIWFDILTPKQLLFFESMIEKLQKSNQILCTSRKYREVVELAK
                     IRGINLVFIGKHGGDDRFGKLNASILRMNGLASRIRKFAPNLTVSFCSPEAARISYGL
                     GIKHVAFCDSPHAEAVMRLSIPLVQKLLIPWIIPKKEFSKYGISQRNIIQYKSLDASL
                     IVKNKSKKYSKKDFSLKNKKTIVIRTEESQAAYISTRKNSFHIVRRIANKFPDCNIVI
                     LGRYTPQINQFKKEFGDKITILNKVVDGKGLLMLTDVFIGSGGTMTAESALMGIPTIS
                     YDAVPNIIEQYLVKKHLVKREKDPEKIVRLVKQFLKSDNKKDINNAKKILKSMDDPFP
                     KLVTAIKSI"
     gene            8030..9166
                     /locus_tag="VLB45_06970"
     CDS             8030..9166
                     /locus_tag="VLB45_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010191291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05473.1"
                     /translation="MGGAKVVVYGLSTEGYAIACQMAIKGADVFIVDESAHMAISLKS
                     EIAKTYPDVASLKEDEPLLGVEPIEVAISHSEYLFFAPRIRKAGQEIKMEIHSKFKDA
                     TSAIKKGGSVIYNLPTGVGGNNETISLLEHVTGLEVGKGISYFYYPLGTKNQVPEIIG
                     SFNGKADDKLAKLLETDKKEKKFVAITSAELFHAIKILSQFSGLSSILEVCKFAQDEN
                     TRNDLMFAEFKNMFLDDMTNNLYDLRSLGTSLEGAGTMMYLVNGSIKGIDGYVKRLID
                     EIRSTLKKNDLKASRTRVALVWTLNPYEMRGDKVDMLNSLVTKLRDYIGDVEIHQGPT
                     FDLFHSDKTTIVVACSKLDHEKINKSGKGSDLIIIKTNPLCEIM"
     gene            9205..9765
                     /locus_tag="VLB45_06975"
     CDS             9205..9765
                     /locus_tag="VLB45_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HSD05474.1"
                     /translation="MSEENDTKTSIQVETSSDDVEESNMDELKGKLARQEETITSYEK
                     KLQYLLADFENLKKRTESDIQNRVNSILSEIVLKFLSIYDDFVRAKEALAKQNVNTEG
                     LDAILKNMDSFLLEYDVTPIDALGEIFDPKLHEAITVKEDPTLDSDTITAEIRKGYIL
                     QNRVIRPSLVEISKISKNNLAGENNE"
     gene            9758..11602
                     /gene="dnaK"
                     /locus_tag="VLB45_06980"
     CDS             9758..11602
                     /gene="dnaK"
                     /locus_tag="VLB45_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HSD05475.1"
                     /translation="MSKIIGIDLGTSNSAASVMMGGKPTTIPAAEGTTIGGKAFPSIV
                     AFTKDGELLVGEPARRQAVTNPTGTIVAAKRKMGTDHIFKVNGKEYKPQQISAFILQK
                     IKRDAEAFIGEKIEKAVITVPAYFNDNQRQATKDAGQIAGLEVVRIINEPTAASLAFG
                     LDKAKQDMKIMVFDFGGGTLDVTIMEMGGGVFEVMSTSGDTQLGGTDMDKVVIDYILE
                     TFKKSSGIDLSKDNTAMVRVRESAEKAKIELSSVTETEINLPFVSYDQTTGPKNLEMK
                     LTRAKLEELINPIVERCRSSMTKSLEDAKLSTNDISKIVLVGGPTRIPLVRKFVASVM
                     GKEPESGVDPMEAVALGAGIQAGIIAGDVTSDIVLLDVTPLTLGVEVLGGLREPLIDR
                     NTTIPTSKGKVFTTAADNQTAVTIHIVQGERPMATDNVSLGSFNLSGIPPAPRGIPQI
                     EVKFDLDANGILNVVAKDLGTQKEAKITITASTKLSKEEIEKLKKDAELYAEQDKKKK
                     EEIDVKNEAEGYIYTTEKLVNQDLKDKIKPEQVTKVNDAVKELKDVLEKGSIEIKPKL
                     DALKSLVNEISTELYKNVTPPPGSGQQTGAEQQAGNPNEQNTSQSSTP"
     gene            11650..12765
                     /locus_tag="VLB45_06985"
     CDS             11650..12765
                     /locus_tag="VLB45_06985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05476.1"
                     /translation="MSNSINFLAIVFSIFLIASTQNYSASAESDTIKFYGMGKVDQSS
                     QFSGEIVRTIIDGDKGTIIHSTDNGLVVVRLELSESEACILKLSTICFDAKVTKVRGT
                     DIQVGDEINLTIDLENKKEFLSVVSGQFQGENIVIDLVKTRTRDNGEFTLMFTREGGI
                     AGMQRKITIDSETKTMLIQNGPDEPSPLVLSDDDITQIRDTIQRNGFFDFNIKKYPPV
                     VGSADYFTYDMEVTAAHFTNKIQWTDTSENVPKKLYLINEKLQNIILSFIDEHPLEIL
                     PLNIAREFVISSPTFAFDGMEETLQVQLSSVTDEATPEYEIHANFDSRHGGYGDRTGQ
                     MVTEAITPHLMVITISDGKVVSAIIDGTWDEINQKLL"
     gene            12768..13856
                     /gene="dnaJ"
                     /locus_tag="VLB45_06990"
     CDS             12768..13856
                     /gene="dnaJ"
                     /locus_tag="VLB45_06990"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HSD05477.1"
                     /translation="MSAKRDYYEVLGLQKNASEAEIKSQYRKLALKFHPDRNKSPDAT
                     EHFKEISEAYAILSDGEKRKVYDQYGHAGVDGRYSTEDIFRGARVNFEDIFGGFGSGG
                     FDSIFESLFGRGGGFGGFGRERGADLIYETSITLEDVLKGKREEIDLQKDVECENCKG
                     SGCAPGTTKRTCTVCNGQGQMRTTRNMGFSTFVTVHPCNTCNGQGKIIERPCSKCKGK
                     GKQKGTKHLSFDIPPGVDSGEYTIRGEGESVESGINGDLVVRIRVKPHEKFKRDGADI
                     FYDANISIVDAALGKTITVPTLEGTEKMTIEQGSQPNTIIKLRGKGLPHVNGRGKGDE
                     YVRLVIVIPTKLDKHQKKLLEEFRDTLN"
     gene            13878..14429
                     /locus_tag="VLB45_06995"
     CDS             13878..14429
                     /locus_tag="VLB45_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05478.1"
                     /translation="MRIGLDVDGVLADVIQAWLSYNNNIRSYISKSDITEWDFWKKFQ
                     IDKFDFYKELSLCWKSWENIPSTEESLSIVTSELSKFGTVDIVTAREESTHDHVKNWL
                     KLKKINYKNYVGVFEGTEKTKLDYDVFIDDSPINAKSMLCEGKSVILYTQPWNLRFDD
                     RRAQRIHQLKDAIPIIKTLIQEG"
     gene            14441..14525
                     /locus_tag="VLB45_07000"
     tRNA            14441..14525
                     /locus_tag="VLB45_07000"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14477..14479,aa:Leu,seq:cag)
     gene            14598..14849
                     /locus_tag="VLB45_07005"
     CDS             14598..14849
                     /locus_tag="VLB45_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05479.1"
                     /translation="MGKIKRVSGRGTSVREEDEKPQNWAGDAYRDVQKKKMESLGKDF
                     KISSGRGTGSKKLQDAPEKPSTKGYYVSTGRGTGKRKLE"
     gene            complement(14956..16860)
                     /gene="gatE"
                     /locus_tag="VLB45_07010"
     CDS             complement(14956..16860)
                     /gene="gatE"
                     /locus_tag="VLB45_07010"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Glu-tRNA(Gln) amidotransferase subunit GatE"
                     /protein_id="HSD05480.1"
                     /translation="MSNLQLDKIGLKVGLEIHQQLATNKKLFCNCSNTESEEYPIKFF
                     RRLRLAKSELGEYDPTAVFERSKAKIIVYHANPSSSCLVEQDEEPPHGVNKDAKEIVL
                     VIASALKSNIFNEIHVMRKLVIDGSNTSGFQRTMLVSQGGHLEVDGIKVGVQGICLEE
                     DAAKILGETIETKEYSLDRLGVPLVEIALEPISGKPEEIKKIALTLGRLLRATKRVER
                     GLGTIRQDVNVSIEGGNIIEVKGVQQLDQLEKVIEYEAKRQHGLLIIADKLKKIETDR
                     IAKERDVKDVTKIFKNCKSKTIQKSLTEGAVVKAIRIKNLSGMFSFEPYEGIRLGKQI
                     GELVRFFGLGGVFHSDELPNYGIENFEIQEVRKLLELEQNDAFLILAGEIRKVDPAIE
                     SIIKRIEDAKIGVPAETRAATVNGETVYLRPRPGASRMYPETDIPPISVTKNELVDAQ
                     KNIPHSWEETISDLQKTYQLNSQLAIQIFDSNYSDLFEKICQDKRISPNFAASILCST
                     ITNLERQGLNAELLKPAEILQIFSFLAEDKITKESIEMIFESIMSGKTHSAVETIEKS
                     AIKKIEPMEIENALEDLVNANIGIIKEQGERSIGPLMGIAMKTMRGKVDGQKLNQILE
                     KKIKEKIRQK"
     gene            complement(16897..18186)
                     /gene="gatD"
                     /locus_tag="VLB45_07015"
     CDS             complement(16897..18186)
                     /gene="gatD"
                     /locus_tag="VLB45_07015"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007549402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Glu-tRNA(Gln) amidotransferase subunit GatD"
                     /protein_id="HSD05481.1"
                     /translation="MSSHSGYKGKTLNFLQKNNISVGDLIIVVTDSKYSGIVMPRYEY
                     SDDEHIVLKLKSGYNIGLEINKIKKVEVESSSSSIKKETIPQSQTNPNLPKILLISTG
                     GTIASKIDYRTGGVTPVLTAAELNANVPEISNIANIDTEVLFSEYSENLLPEHWKKIA
                     EKINSYTKSEYIGIIVAHGTDTMQYTASALSFALAGIPIPIALVGSQRSSDRPSSDAA
                     TNLIAATKFLIECNIGGIYVVMHNTTSDDEIACHWGTRVRKNHTSKRNAFQTVGGNPA
                     FIVNQDKIEKNPQFNYTKKDYLPRINFDTRVALVKYYPGLSPKIIDHIIEDGHKAIIF
                     EGTGLGHVGKVMYDSVSKAKKKGLFIGMTSQCIDGMVSMTVYESGRDLLSLGVIPLAN
                     MVPETALVKTMWALGNSKNHQEMEKLMRENIALEFSD"
     gene            complement(18219..20420)
                     /locus_tag="VLB45_07020"
     CDS             complement(18219..20420)
                     /locus_tag="VLB45_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007403162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDC48 family AAA ATPase"
                     /protein_id="HSD05482.1"
                     /translation="MSQNTLSLKVLEAYTRDVGRGVARIDYDSMDSLSASTGDVIEIK
                     GKRRTVAKCLPLYPSDEGKGIIRVDGLVRNNAGIAIGDTVAVRKIKAVAAEKVVVAPL
                     EAIPPIDERYLADALESVPLIKGDNVMVPYFGGRLTFQVIGVTPAADAVLVTQKTVFH
                     IAEKGETLRGVPQVTYEDIGGLTDEIKKVREMIELPLRHPEIFEKLGIEAPKGVLLYG
                     PPGTGKTLLAKAVANESNAHFISISGPEIMSKFYGESEARLREIFKEAKEKSPSIIFI
                     DEIDSIAPKREEVTGEVERRVVSQLLSLMDGLEARGKVIVISATNRPNAIDPALRRPG
                     RFDREIEIKVPDKKGRKDILMIHTRNMPLSDDVNLEKIAAISHGYVGADLEYLCKEAA
                     MKCLRRLLPELNLEDEKLPPETLDKLIVNYDDYQNALKEVTPSGMREVFIENPDVSWD
                     EVGGLETVKRELQEGVEWPMKYPTLYSKLGHRMPRGILLHGPSGTGKTLLAKAVATES
                     EANFISVRGPELLSKWVGESERGIREIFRRARQASPCVIFFDEIDSIAPVRGVGGETA
                     VTERVVSQLLTELDGIESLHGVVVLAATNRADMIDTALLRPGRFDKIILVPMPDKEGR
                     KKILEISTKEIPIDREQKTEAGKNLNYVDLEKIADTTDGLSGADVASIANTAVSLVIH
                     EFLDKYPDQKEAEKQAATAKVTMRHFEEAVRKVRTQKELKIGEKVAVPYYR"
     gene            20525..21271
                     /locus_tag="VLB45_07025"
     CDS             20525..21271
                     /locus_tag="VLB45_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HSD05483.1"
                     /translation="MGKRPLVRRRGRGGMQFRAASTGKILPAKYPRFGLAEQREGQVV
                     DLVHERGRDTPLAKVRFENGSFSFLPAILGTRIGSKIQFGLKSEIAPGNVISVQNIPD
                     GTIVSNVEKHFGDGGTIVKSAGTYATVFSHSADGVTIKLPSGKFTTLNPQNRAMIGTL
                     AGGGVGDRPFMRAGNAWRRKVSKGHKYPIVRGVAQAVYVHPHGGGRHQHVGRSSTVSR
                     NAPPGRKVGSIAARKTGRARVKDRPDSSSS"
     gene            21319..21597
                     /locus_tag="VLB45_07030"
     CDS             21319..21597
                     /locus_tag="VLB45_07030"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HSD05484.1"
                     /translation="MANQRVHIFVKGKVQGVFFRQTTKNVAEKKNVTGWVRNLKDGRV
                     EAVLEGQDINVSEVVEWGHRGPPNAVVEDVQIINEKYKGEFSKFEILY"
     gene            complement(21594..21926)
                     /locus_tag="VLB45_07035"
     CDS             complement(21594..21926)
                     /locus_tag="VLB45_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5655 domain-containing protein"
                     /protein_id="HSD05485.1"
                     /translation="MGDLIFSGNNRNYQDFQKQLTPLIKPLFDSLREYCFSLGENVVE
                     DVRMHRVVFCKSMTFRFFADMEPQKDSILIKIRRDRKEPQKEIEIKPSQDLNEIKNLL
                     SDAYKTIH"
     gene            complement(21954..22269)
                     /gene="ffs"
                     /locus_tag="VLB45_07040"
     ncRNA           complement(21954..22269)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VLB45_07040"
                     /product="signal recognition particle sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01857"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="archaeal-type; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01857"
     gene            complement(22304..23218)
                     /locus_tag="VLB45_07045"
     CDS             complement(22304..23218)
                     /locus_tag="VLB45_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00269 family protein"
                     /protein_id="HSD05486.1"
                     /translation="MKCDRCDNVAAYSRKYSGENLCSTCFSNSILQKTARIISKYNMI
                     QNGEVVGVAVSGGKDSLSLLHVLKKMSESHNFTLRAITVDEGIPGYRDEALQIVKNFC
                     SSLGVEHRSYAYKDLFDMTLQESLELRENEKTTSCSICGTLRRRAIDIGSKDLDVDVI
                     ATGHNLDDVLQTFIINVLSGDTNKIGWMDPDTSTNKIRKIKPFCEIYENEIAFYAFTN
                     DIPFQSEQCPHMNEGIRTEIREFLNSLEKKHSGVKNNMHRSITRISHIIKDTNYKQRR
                     TCPKCGNECTGEICSVCDMLVNLKQDRS"
     gene            complement(23281..24483)
                     /locus_tag="VLB45_07050"
     CDS             complement(23281..24483)
                     /locus_tag="VLB45_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007403155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HSD05487.1"
                     /translation="MAIDFLTQNSIFYVLIAWVVVIILAKASKLDKHGFDIKPYSLTY
                     KNYKVQLALTRLLGKTEKATKIFANTSVIAGFVMMGFAFWFLISNLSNFFVKPESFSE
                     MTVLIPGVTIQSGINIAYFLLTVPIVLVIHEGAHGIVATLEKIKIKTGGFAVFIALFA
                     GFVEPDEEEFAKAKKISKLRVIGAGATSNVLFSFIIAGLLIFNPAFALLMPEPIRDVF
                     YKDPLGVPILSVTEGSGAAMAGLLKDDIITAINGVSVIQPQDFAKVKLAPGETAIVTL
                     IRKGETMQIPVITKAAPDDPQKGLLGITRGTFPAYQPTVPYYIPWSPEVFMFLLWLWM
                     LSFFIGIFNMLPLPILDGGKFIQSIIEKKISDKVLKVTMLSIYAFTFAIFGLNIAFSA
                     IKSGFVTI"
     gene            24528..24848
                     /gene="cutA"
                     /locus_tag="VLB45_07055"
     CDS             24528..24848
                     /gene="cutA"
                     /locus_tag="VLB45_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HSD05488.1"
                     /translation="MVAIVIISTYPNKKSILKIANCVVKKHLAACVNFTKIDSIYIWQ
                     NKLENTKEFLALFKTTNTKKEILKKEIKKLHPYQVPEIMELKVNSVNKSYLGWLVNST
                     RDKT"
     gene            complement(24828..25877)
                     /locus_tag="VLB45_07060"
     CDS             complement(24828..25877)
                     /locus_tag="VLB45_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mRNA surveillance protein Pelota"
                     /protein_id="HSD05489.1"
                     /translation="MITKTIDECSVALIAEEPDDLFTLRRIIKKGDRVVGDTTRAMKQ
                     EKDFSRPDRGERIKIRVALNVEKISIDDVVDRLKVHGTIIESDSESVQKGSHHSLTLK
                     IGEVVTLTKKNWTNIEKKLIIEKKEGHSFVLVAIDTADCGIGRLSGTHLKLMPNMYSG
                     AGGKRYKSKFNIETFFADVENAISSSVREGDLIVIFGPGETKKKLGNYLAETPTGKKH
                     KIQVVEGIDSGGEDGIYTFVKSETMKQVMAQSKIAKVAKIIDEIMLMVNKKSNKFTMG
                     YNETTKANDAGAVESLIFSDKIFEKQDEDQVIEFLNQAESRGVEIFSVDSTTDVGLRV
                     SSLGGIVSLLRFVPS"
     gene            complement(25923..26345)
                     /locus_tag="VLB45_07065"
     CDS             complement(25923..26345)
                     /locus_tag="VLB45_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05490.1"
                     /translation="MVTIDATCSLEGFRKFVILSTCNSFIPESYFEDYEVFPEREESP
                     GLIYVEAADKVTLKKIRDMTFVNAKDVFGIIYSSKSGNTNLKWRQIRRNSGKVTGEAS
                     SNTLVNLTEAGVITQEWVQNYLRKKAGEKQTKASELTN"
     gene            complement(26935..27849)
                     /locus_tag="VLB45_07070"
     CDS             complement(26935..27849)
                     /locus_tag="VLB45_07070"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010192205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HSD05491.1"
                     /translation="MKEQGKIWMNGKLVPFKDAKVHVLTHALHYSTAVFEGIRCYNTP
                     KGSMIFRLPEHVDRLFRSAKMYSMKIPYSKQQISNAIIKTVKASKLRECYIRPIAYYS
                     YGVLGLTPTPNRIDVAIACWEWKLGEGSAGKVRGARCKVSSWLRIDSRSQPMQAKAAS
                     NYANAALARVEALKDGYDESIMLNYHGKVAEGSAENIFLVNDEEISTPPLSAGILNGI
                     TRDSVIKIARSNGISITERNIDRDELYIADEVFMTGTAAEVKSVTEIDNVTIGNGKPG
                     KITRKLQGAFMDVALGKDEQFSEWLTPI"
     gene            27982..28194
                     /locus_tag="VLB45_07075"
     CDS             27982..28194
                     /locus_tag="VLB45_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05492.1"
                     /translation="MEQVLSFVAVGLLVFGLIGQGFELRKMRIAALSDGNISSSNMFT
                     NKKNLKWYIIISAGLIIWYVSGGFTR"
     gene            complement(28356..29771)
                     /locus_tag="VLB45_07080"
     CDS             complement(28356..29771)
                     /locus_tag="VLB45_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HSD05493.1"
                     /translation="MKNLVVAKFGGSAIGIDGKMIPVIVKRINQLKSDSKVIAVFSAP
                     LTLYEGKQRSLTDIALMIGQKAQTGQIPNMDELKKPYQKILEFVNKEHQDKCKKTIDS
                     FLEKASKSIKQAAEKKEFSDVIRSSTLAYSGEILMSYVMDYVLKSSNIKSEAVGFDIW
                     PIITDKNIESANFLATHSAQSMNGVEALLEKNDVLSIGGFIGKTTDGLETTYERGGSD
                     RTAADLGILFSKKYNTKIDFEKDNSVLSADPRIVKEELDEIDTLSYNEARIAGMFGMK
                     ILDPIAIKEIVENGIDIPIIITDMNNPKKTTTIERKPKSKNGHPLKIVTGKKNCAILR
                     IESTAAQKLFESLENEKRYSEFVVLSPFTKDDVEFTRILFLDADYIKRNERYVLAFDS
                     LATITYNRGVITLIGDEMWRVQQIASKASSKIGDAGLNILNMDAQEETSRIIIVIEDV
                     SDNIEKAICTVHAERSKIKFV"
     gene            complement(30115..31128)
                     /locus_tag="VLB45_07085"
     CDS             complement(30115..31128)
                     /locus_tag="VLB45_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HSD05494.1"
                     /translation="MVDVAFFSSPIGLGHATRDVAIAQCLDHTRIKFVSGEGAYALFS
                     DCGFQAENAYRPPFFEIKNGNLKNPLRWLFRYYSYYKECKTISSKIIEDENPRLVVSD
                     EDFASLGIAQEKKMKTVLITDVLKTSFVKGIGSMIEKKMNHSMMNMIQKCDLVILPEN
                     GPSEGNIMRVGSIVRKTNHSREELRKKFSFNRKTILVSIGGTSSGQFLIERAMHVFKE
                     LHLDADMFIVSGPSLKISDSQIRNLGFVNNLHEFIYASDLIISLAGKSTIDEAKYFGT
                     PGIFIPIKGHFEQEENAKQIGFSFEDLDHLESLIPNKLNEKRDPQMFDGAQKTADLIK
                     ALL"
     gene            31219..31292
                     /locus_tag="VLB45_07090"
     tRNA            31219..31292
                     /locus_tag="VLB45_07090"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31253..31255,aa:Val,seq:tac)
     gene            complement(31293..32171)
                     /locus_tag="VLB45_07095"
     CDS             complement(31293..32171)
                     /locus_tag="VLB45_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010190682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HSD05495.1"
                     /translation="MLSYDYPLYRPPSEANSLIFQVTLGCSFNVCSFCNMYRTKEYVE
                     RPWDEIKMEIDLAAKHMPDTQRIFLADGDALNLPTERMIQILDYIRQKFSKIERVSCY
                     AMPKNLLEKSAEDLKKMYDAGLTMFYIGIESGSDKILKKVTKGATGQTIIKACQKAKD
                     SGYVISCMIILGLGGKTHSKEHIDDTAKVISATSPHYVGALTLHIEDGIREEFMTKFK
                     EPFIPIDDADALVELENMVSKIETKSPIIFRANHGSNAYPIGGTFPEDKNMILEKISY
                     LKEHPELCRPQGFRGF"
     gene            32267..32953
                     /locus_tag="VLB45_07100"
     CDS             32267..32953
                     /locus_tag="VLB45_07100"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HSD05496.1"
                     /translation="MLNTELPRDLVPKFFDKTARTYDKIVNLTTFGRDKYWKKEILKK
                     IQNCNSVLDLACGTGILTFQIAKQLPNAKITGIDVTESYLDIARTKLKPHHNISFLHQ
                     DAEKLDLNTKFDCITSSYIPKYCKSETLIKVCIEHLNPGGKIILHDFTYPQNKLVRTL
                     WDFYFIILNLMGFFIPNWKKVFSDLPNLIRTTRWLDEYENAMRAHNLSTVRQSLTWES
                     SAILTGTKMV"
     gene            complement(32921..33358)
                     /locus_tag="VLB45_07105"
     CDS             complement(32921..33358)
                     /locus_tag="VLB45_07105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05497.1"
                     /translation="MAVVLDLLSKALLNTPVGHVEQTVSEILKKPVQFRVIEQHPISE
                     TEYIRKIVISSEQFPIILATVKFDSKILPKQIMSELLLKKEGIGKILYKYKIKVQRKM
                     GNIEIAPDTKKITRDYEIVHDERIWFQIYEEIRLDHLSTCKNS"
     gene            complement(33383..34069)
                     /locus_tag="VLB45_07110"
     CDS             complement(33383..34069)
                     /locus_tag="VLB45_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05498.1"
                     /translation="MSKDRLSEWFVPKFGSKNFRVGIGILFLPYTGMVVSFAAWGSFV
                     AGFSVERLAAICILYFFALGISAHCLDSLGSKNKPWGTLSKRNVLLISLLSLAIAFVI
                     GLYYAFLDSPLLFPIGIAESFFLFAYNLEWFKGRFHNNISFVISWGVLPVFAGSAIQA
                     NTISSETLVVAGIAGILSYILIKTSRKYKELKHQESSYSETRRKETVLKLISIGVIVV
                     TVSYFILRQT"
     gene            complement(34105..34845)
                     /locus_tag="VLB45_07115"
     CDS             complement(34105..34845)
                     /locus_tag="VLB45_07115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05499.1"
                     /translation="MIDSLLFLTSLFLGFRHGFDYDHMVAITDVTNTQARKKQAAFLT
                     LFYSLGHALVVISIGTSIILLGISVPQILDEVIGKAVGVTLIILGSYIVYHLAKKGQK
                     EFRAATRFTIIANGILFIYSKLGSKLFGRTIPQRRVFENGYGQHSSFLIGMIHGIGAE
                     SPTQLAVFAMSLGLGMILGVTALVVFVAGIIVSNLVFGIMISTGFIQSLKSYKIYKYA
                     SIASAATSLLVGSLMISGFDFILPEIPS"
     gene            complement(34887..35408)
                     /locus_tag="VLB45_07120"
     CDS             complement(34887..35408)
                     /locus_tag="VLB45_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010190694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AsnC family transcriptional regulator"
                     /protein_id="HSD05500.1"
                     /translation="MSDFTNVKQHNLNICKMYKYGKNLQVDMPFKLDDVDISILESLV
                     KDGRKSFRQISREIKVSTPTVKARYERLINVGLIKAVLPVLDLGKLETKTGTRLGQIH
                     DKTIKNHNVKLRKEMLVKMSCDYCKGQVEGKPSILKFANFERFFCCTSCRTLYKEKYK
                     GRISSLSNTKSSF"
     gene            35438..36433
                     /locus_tag="VLB45_07125"
     CDS             35438..36433
                     /locus_tag="VLB45_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007549540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HSD05501.1"
                     /translation="MSIQVTARKLTTIVSFILFSLVFLGIIFSLTNDVMVKGNEDVSY
                     LSWLVISYVAGLSMIILPCTLPLVFIIVPLSMGQNSKKGLVMALLFGIGLTITITAYG
                     TGVAILGKTASLDQISVVMFLIAGIAAFMFGLSQLKLVTLKLPSYSGTPKFIQNRGDY
                     SKSFFMGILLGNAGVGCPNPLFYWLLIHIANVGSIEVGASLGLVHGVGRAIPLILMSV
                     LAIMGINATKNLTSKRVPIEKATGWMLVVIGSFLIINGLPSGHQWYEETFVHIAWNNL
                     VSITPIPPEFHVGQHMHENVYGIEISKNYIPTLLFGLIMTPVLWYLIKKRKVVIS"
     gene            36430..36573
                     /locus_tag="VLB45_07130"
     CDS             36430..36573
                     /locus_tag="VLB45_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010191157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing protein"
                     /protein_id="HSD05502.1"
                     /translation="MKDPVCGMDVGEKGESLTHDGKEYRFCCASCRWAFEKNPEQFVG
                     KES"
     gene            36570..36803
                     /locus_tag="VLB45_07135"
     CDS             36570..36803
                     /locus_tag="VLB45_07135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HSD05503.1"
                     /translation="MKVEILTTPGCSNCSVVEKMFDEAGIPYKVIDVTENPEYLQKYP
                     IYTAPGVVIDGKLEFSGVPRKKEFNEKLEKLRS"
     gene            36855..37307
                     /locus_tag="VLB45_07140"
     CDS             36855..37307
                     /locus_tag="VLB45_07140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05504.1"
                     /translation="MKQEYKEYLKLNKNIILGFAASICVSTVAAHLFSEQEAYLNATF
                     TLVVDYVVYFTTFGGLYYIDNRKKYRLESGETDKISLRKDLIKIISSLGIGEVVYTIA
                     RWFLQYYFLTIGYDAYLASLVSQSTSTVIYMLVVNISVKLTRLYKDGT"
     gene            37297..37653
                     /locus_tag="VLB45_07145"
     CDS             37297..37653
                     /locus_tag="VLB45_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase-like protein"
                     /protein_id="HSD05505.1"
                     /translation="MVLSIYVDGSGGSESGFGFFVKETGESFYKKESNITNNQAEYMA
                     ILAVLKKFTNYTDEITIYSDSKNTVSQLNHEYAINSPELRTLAMEAWNLIGKYSNLKI
                     KWIPRKENLAGKMLGS"
     gene            37735..39840
                     /locus_tag="VLB45_07150"
     CDS             37735..39840
                     /locus_tag="VLB45_07150"
                     /EC_number="6.2.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010190675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043758 - acetate-CoA ligase (ADP-forming)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybutyrate--CoA ligase"
                     /protein_id="HSD05506.1"
                     /translation="MAESVFLSPKSIAIIGASDKEGSVGRAITSNIMKGYTGKIFPIS
                     PTRDTVFDKKAYKSVLEVPEEIDLAVVITKNDIVPSVLEECGKKKLKGVIVITAGFKE
                     VNEQGAALERKLGEIAKQYNLRIIGPNCLGVMNLAPETMMNSTFLKVTPKSGGIALVS
                     QSGAICAALVEDASAQGIGFSAVISMGNKVDMNEVDILQMLAEHGQTKVIVMYLEDLG
                     KGPEFLKICKQITRLNPIKKPILVLKSGRSPEGAKAAMSHTGALMGSDEIYDALLKQS
                     GAIRVDTMEELFDYATAFSKQPLPTKGDLVIVSNAGGPAIISTDACSKLGIKMAKIEE
                     IRPKINAVIPPWGSSRNPVDIVGDADFNRFNNVLNEVLAHPNVGSVIAMCTPSATLDY
                     NKLAEVIVDVSKKYNKTMFASLMGLDEGIKNKEILAQGGIPHYNYAETAIRSLKAMLR
                     FTEWSQATEGTVRKFAVNKKKVEKVFAQVKSEGRKNLLEEEGQEVLRAYGFPVPKSIL
                     AAKNSEAVSAAKKIGFPVVMKIASPQIIHKSDAGGVKVGLKNPKEVKTAFKEIIKNAK
                     RYDKKAIIKGVLVQEMVKGGKETIIGSKQEPGFGPVVMFGMGGIYVEVLKDVTFRLAP
                     VTDKEADEMISSIKTNKLLQGVRGEKPSDINKLSECIQRLSQLVTDFTEIKELDMNPV
                     LVFEKGKGCKAVDVRIGLT"
     gene            39910..41436
                     /locus_tag="VLB45_07155"
     CDS             39910..41436
                     /locus_tag="VLB45_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase family
                     protein"
                     /protein_id="HSD05507.1"
                     /translation="MPIRTGAEYIESLRGRKLKVYLFGERVKEPVDHPIIRPSINAVA
                     ETYDLAVREEELATVKSSLSGQKINRFLHIAESAEDLVNQNKMQRKLGQNTGTCFQRC
                     VGMDALNSLHSVTFEIDEKHKTSYHKRLIDFIKMVQTENLVIGGAMTDPKGDRSKAPH
                     EQEDPDMFVRVVEKNDKGIVIKGAKAHQTGCINSHWIIVMPTMRLQENDTDYAVVGAV
                     RADDPGITYIYGRQSCDTRSMEEGDLDSGNSKFSGQEAMIIFDDVFIPWERVFMNGEY
                     EFASMLVERFTCYHRRSYVCKTGLGDVLIGAAASIADYNGIPGVSHIKDKLVEMTHLN
                     ESIYAAGIASSYQAYKTKSGVWINDDMLANVCKHNVTRFPYEIGRLAQDIAGGLLVTL
                     PSEKDFRNPETGPILKKYLKGRKGVDVENRMRILRLIENMTMGRNAVGYLTESMHGAG
                     SPQAQRINIARLMQLGYKKKLAKNLANVKEEPDLTPEQAEYFERVFKISKSVAGVKNN
                     "
     gene            complement(41439..42095)
                     /locus_tag="VLB45_07160"
     CDS             complement(41439..42095)
                     /locus_tag="VLB45_07160"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HSD05508.1"
                     /translation="MSSITKDIIIVVIGVAIIWFGLRIVFGTDNPFYVVSSGSMEPNL
                     NVFDVLVVQGNDPFKELKKGDIIVFDRPSGHDRVIVHRVAEILDEDPRVIRTKGDANP
                     ASIPGTDFPITEEEYIGKVAYVVPQIGYVTRILTPPINYIIIAVIVGIMILKQYGKHK
                     GRSDNPSDESPKDQPPTDDYNTPSPGITSAEIEHVQDNEYTKTDSIKEISEAKESEQK
                     "
     gene            complement(42165..42902)
                     /locus_tag="VLB45_07165"
     CDS             complement(42165..42902)
                     /locus_tag="VLB45_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05509.1"
                     /translation="MSSELRLKKLRGSGGYIMASVTDEQQRKGNLGGPDLFLAPVGRI
                     DVNRISKYFCNTCEKDFEGSPKIESETPNELVAENLMLVEKGQYVCTNCGSVLAEFRT
                     FSKPNESADVGLAKPQSLATQILVESSPASDYMQPQHTYSQQEAPPEVTTFNSITGIA
                     VYDSDARKVGTVKQIGLQSGQSGIVLVVTKNDGSDIMVKWEEIRKIGEIVLLGNQVSD
                     TSSKCNGCGFENKPDAKFCESCGKKLK"
     gene            43140..44438
                     /locus_tag="VLB45_07170"
     CDS             43140..44438
                     /locus_tag="VLB45_07170"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007550997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HSD05510.1"
                     /translation="MTKFEELGIKKSILDGLKEINFQDTFPIQAAAIPVILSGQDVIG
                     QAHTGTGKTAAFSLPILQNIVPKGGIQAVILAPTRELAVQISGEISKFAKYTGIRNVT
                     IYGGQSINNQFRDLDRGVEIVVATPGRLIDHLKRGSIELNDVKYVVLDEADTMLDMGF
                     IDDIKFILDLAPDERSTSLFSATMPAEILRLAEKYMRNPQQIFIDADDLSGEGIDQAF
                     LVIRDREKTDFLFKFIKENKNGQTIVFCSTKDRTRRVAHELGKENFNVVSIHGDLSQF
                     RRDKAMYLFKKGKVDILVATDVAARGIDVPEVALVINYDVPNQNMVYFHRIGRTARAG
                     AKGRAITLVSYSSVGDFQAIARQTKVQMTDLNKELGITVKIPDPLKRDPGHRGGGHGY
                     GRRYGDDRRYGGERRYGNRQSHGDRGSSYNKKYYGLRSRW"
     gene            44501..44833
                     /gene="trxA"
                     /locus_tag="VLB45_07175"
     CDS             44501..44833
                     /gene="trxA"
                     /locus_tag="VLB45_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HSD05511.1"
                     /translation="MGNAKAVDTKTWQTEVMQSQLPVFVDFWAEWCGPCRMVGPVVEE
                     LAGEYSGKVSFVKVNVDESGELASKYNIFSIPTLAIFNNGEIIAQQVGAASKDSYKNM
                     IENALKKI"
     gene            44904..45422
                     /locus_tag="VLB45_07180"
     CDS             44904..45422
                     /locus_tag="VLB45_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HSD05512.1"
                     /translation="MSFGEVDTLNLLFDKLQNLLNDSQGYYESFLDANTLYKQGKLSE
                     KEFFEKLGDYVVAYSALEFLAVKVIFELKKSIDKIASGSTGTTSGSTTVGMGGFGMTP
                     PAGHMMAQNAPMPGRPPSIISASAFNAPGALPTPDPSLLPPRTNLPSCTSCGSAIRPN
                     SKFCTKCGNKIQ"
     gene            complement(45473..45838)
                     /locus_tag="VLB45_07185"
     CDS             complement(45473..45838)
                     /locus_tag="VLB45_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HSD05513.1"
                     /translation="MKVFNGKAAAEDYMSNHTLTFSTPDLTLTRFAFWLGDIIPDPKN
                     PGQTMPRMMAYVEEKDFAPVQIIDDEYVPTGAVKSSSGGIYGSLKTESGSDTKFCSEC
                     GIKISSNAKFCTECGATQD"
     gene            complement(45884..45956)
                     /locus_tag="VLB45_07190"
     tRNA            complement(45884..45956)
                     /locus_tag="VLB45_07190"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(45921..45923),aa:Ala,seq:cgc)
     gene            46036..47430
                     /locus_tag="VLB45_07195"
     CDS             46036..47430
                     /locus_tag="VLB45_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HSD05514.1"
                     /translation="MTFDTVITDSHVVLPSGIVEKNIVIDNGKIVNLTNDIPQCDVKI
                     NGRGLVSMPGAIDPHVHYGVYSPIEKAAITESRVAAIGGVTTMMRMLRLGGSYKKLLP
                     SHLSQSATTHYVDYAIHASILQKDQIDEMEFCTKNGIQSFKIYMNLGEDIGHVYMDME
                     PETNQLHEEKVEMNLDMIEKIIANAASLGCPVLVHAEDYQMCSCGIREAREKNKDGLG
                     AWSESRSVESEVKSIRTISKIARKIGCTLYFVHIGSEGAMNAIKDEKKNGTKIHVETC
                     PHYLTLSFESQNGYLAKVMPPIRTSKDVDAVWKEIHNGMVDTIGTDHVANQLKLKLGG
                     NDVWGALAGFPGIGTMLPILISEGVNKGRITLQQLMEFTSMNASKIFGMFPKKGILQK
                     DSDADIVMIDMKKENKVSSQLLDGFSDYTVYDGWNLKGWPVKTIVRGEIVAEDFEIIG
                     KQGHGKYVTRSNLR"
     gene            complement(47422..48099)
                     /gene="rpiA"
                     /locus_tag="VLB45_07200"
     CDS             complement(47422..48099)
                     /gene="rpiA"
                     /locus_tag="VLB45_07200"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008297758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HSD05515.1"
                     /translation="MTIDDAIKALSADTLRLIQDNYVLGLGSGRAATALVKSLSSYLK
                     EKNIRVKAVPTSLQIKLEAEKGGIPIIEADQIEHIDLVFDGADQIDEEKYMIKGGGGA
                     LLRENILINIAKKVVIIADESKFVKKLNHSVPVEVHPFARNTAARHIQKYSGKPTLRT
                     IERGYPFVTENGNIILDCDFGEISKPRELAEKIIQIPGVVEVGIFTRKPDIIYKVKEN
                     GKYDILT"
     gene            complement(48100..>48785)
                     /locus_tag="VLB45_07205"
     CDS             complement(48100..>48785)
                     /locus_tag="VLB45_07205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05516.1"
                     /translation="VIAKIEKLEAASKHPDLGPLGDGLITLMDQKLSQLDQRLHEISS
                     KITVATMTPPQIKQLEPPKESIPEKPIQKKIEIKNEIQKPKEPYTPLPPIEVAIAAPH
                     KSVELITLTEIPNRMSEFPVELVKPVVVTPQKQIIEEIMTSRTAEPEKPKMNIQSVVE
                     TPKIEIQPVIETPKPEPAQIPVEKKIQIPAAVKIPEDEKLEDDDKDIDKIKNEIMKAL
                     SKLEQVEVE"
BASE COUNT        15441 a         8830 c         8824 g        15690 t
ORIGIN      
        1 ttactagata atgtgatagt gggtagaata acaaatactg cagagcaagt aaaatactta
       61 gaaaagacat tagaaatcaa ggatctctat ctcgttgtaa tagataatat cacagatcta
      121 ttttcttttg aatactcaaa agaatcaaaa tcattaacaa agcatatcga gtttatgaaa
      181 tacatgcata atctgtcatc tgtagccata aagaaaaaga tagcaattgt tgtaactaac
      241 atggtaagaa aatcagatga aaaggaaaga gaaaacctgg atagatcgat aagtatgtcg
      301 gtgcatcaga aaattcatct tgttaaaatt ggtcacaaat atgtcgcaga agtaagacca
      361 tcatttggga ttaaaaaaga aatcccttac atgatcactc catctggtct tgtagattca
      421 tcttaagata tttatcactg taatcagtaa tgaatccatg gcaggtattt ttgattcaat
      481 tccaatcatt gctgttattt tatttggaat tggacttgtt ggggtaatgg tatacgagcg
      541 agctagaact aggcaaaatc aggaaccgcc tacctgaatc tgaactgata accaagtttc
      601 attcccttgg ttttgaaaac ctaactgaca ttcagaaaaa agcagtccct ataattttcc
      661 aaaagattga ttcactagta gttgcaccaa caggttctgg taaaacagag tcgtccgtaa
      721 ttccaatttt tgcaagaatt gcaaaatcaa aaaaaccagg aaagataaag gcattataca
      781 taacacctct tcgtgctctg aatcgtgatg tatttagaag aataatgaaa tatgctgaac
      841 aagaagctct ttcaattcag atacgtcatg gtgatacttc tcaatcaatt cgaaaaaaga
      901 tcagtgattc tcctcctgac atactcataa cgactcctga aactattgta atattactaa
      961 ctcaacaaaa aatgcttcat gccctatctg aacttgagtg ggttgtaata gatgaggttc
     1021 acgagctttt aggaaatgaa cgtggagcac aactatcatt aagcttggaa agattacaaa
     1081 ataattcaaa atctgatatt actagaattg gtctttctgc tacagtagga aatattgaag
     1141 agtgtgccaa gtttgttgtt ggcacaaaaa gaaaatgcaa ggtaattcgt gatacatcta
     1201 ttagaaaata cgatgtagaa ataaaataca ttgatggtac aataactgat gtagttgatt
     1261 tcataataga atatgtggca aagaactacc aaaattcccc agtcttactt tttacaaata
     1321 ctcgtggtga atcagaatac attgcttcaa ttctacgtga aaaatctaca tctagaatag
     1381 aattacatca tggctcactt tcacaagaag taagagaaga aactgaggaa aatttgaggc
     1441 aaggaaaacc cggaattatt gtttgtacat cttctcttga acttggactt gacataggtt
     1501 ctgttgaatt ggttattcat tatgggtcac ctagacaagt atcaaaacta gtccaaagaa
     1561 taggacgaag cagacacaac agagattcat ctgccaaagg tcttgttatc acaaataatc
     1621 ctgatgacga atttgaggct agggcaatac ttgagagagt gaaagaaaaa tctatagaag
     1681 atcagataat tcatgctgat tcgctagacg tactggcaca tcatctagtt gggctaacca
     1741 tgcaatttgg aagtgtaccg gtagatctgg catttaagat aattagacag gcatatccat
     1801 tcagaaatac aactctagaa gatctttttg gcgttcttga tctcttgcat tctagctccg
     1861 tcatattttt tgataaagaa gaaatgcttt ttaagaaaaa aggtagggcg tttagatatt
     1921 attttgaaaa tctatctacc attcctgata ttttaaaatt caaagtgttt gatactgcaa
     1981 gcaagaagat aattggaagt ctagatcagc gatttgttgg cgattatggt gaccctggta
     2041 atgtttttgt tctgagggga atgcaatgga gaatattaaa tgtggatgaa agctcactca
     2101 aaattaatgt agaacccata caatcggcgg gaattaatgt gccatattgg gaaggtgaaa
     2161 acattcctgt tgattatgcc accgcaagta aagtgggttc atttcgatca aaagtacaga
     2221 tgaaatcaat ggttgtctta aacaaggtaa ttgaaaatct taagctaagt gaaattccaa
     2281 atgaaaaatc tgtcgtagtt gaatcattaa gatctagaaa tatggttgta attcattcat
     2341 gttttggaac aaaaataaat tcaaccatct caatgctcct ttctacaatg ttatctgcca
     2401 aaacagggca tctcgtagac acaagatcag atgcatatag aatagttctg tcatcaacag
     2461 gaagaatacc agaaaaacag gtgttagaca tactaaatga agaatatgat ctaccattag
     2521 ttgtaactgc atcacttact ggaacccata acataaactg gagaacctgg tgtgtagcca
     2581 aaaaatttgg tattgtggga aggggagcaa tttatgatag aaaatctgca cgattcatct
     2641 atgaaaaata ctcaaaaacc tcacttgcaa aagaagcttt acgtgaatta tttcatgata
     2701 aatatgactt gaaaaacaca caaacattat tagaaaaaat caaagcaaaa gaaattctat
     2761 taaaatggat agaagtagat agcttttcaa aacttgcaga atccatactc gatcatacca
     2821 caaaatacta tgcatctcct gcaaatattg acaaaggaat attggatctt gtaaaaacaa
     2881 gacttttgaa aacaaaacat aggcttatct gcgctagatg tggaaagtgg gaaaaagttg
     2941 tagagacaaa agaagtaaat gatgttcttt catgtcatta ttgtaaatct cgtcagataa
     3001 ctgcaacatt ttattctgat tatgatcttg ggaaaataat acaaaagaaa cattctggca
     3061 aaaagatctc aacagaagaa aaccataagt tttcgcgtgc atggaaaatc tcctcacttt
     3121 tggaaaactt tgggaaaact gctcttatag ttctctcagg ctatggagta ggtgcagata
     3181 cgggtgcaag aatactccga aacatgatcg gtgaagagga gctatacaag caaatctatg
     3241 aggcagaaag acaatatgtt acaactcgcg gtttttggga ttcttaattc ttttttgtta
     3301 tttgtgaaaa gggggtcaag aataattcga ttctgccctc gagcccagct tttgcggtta
     3361 ccccgattat ggaaacgatc tctccttgag aaaattgatc aagcattcgt gcctgatttc
     3421 tccatccttt taaccagatc tgacctgtct catcttcgat aaacatctct gataatgcaa
     3481 cggattcacc tgtttttgtt tgaatttcac gcctttctgg cattttgaga attatggcct
     3541 cgacgcagta tgtattatca cttggtttta tgtctcgaat ctttgttctt atgtctgata
     3601 tggtagggat attttcatca tttatttttc tgattaatgc atctttttct aatgttatgg
     3661 aatttccata agcttttgtt ggcatacatt ctatgacttc accaacttgc atatcttttg
     3721 aaacatttgc aaaatcagta atgtttaaca atttcttttt ttggtctact accaaaatta
     3781 gattattccc ataatcgttt tttgatattg ataaaactct aacagttaaa ggttgtattt
     3841 catttgcgcc ctctatttca ataacagttc cttcatctcc atgcagttca attccttgct
     3901 gtcctctttt tgttttcaca ccgattagtc ttgtcttcgc accagaagta atcatttttg
     3961 gtattgaatt ttcatcggtg ttccataaaa caaccctgat aatcgtgcca tcttttccct
     4021 ttaaccttag atgcaaagct tttccaggtt gacctttaaa gttggtgaat tcagacatcc
     4081 taacattgcc atccaacatt ccagaaacta caagattttg ttgatcctct tttaccatgc
     4141 ttacatcttc tgttattgag tcaagcaatg gaatgctgct tttcataccg cttgtttcaa
     4201 gattagaacc agaacctata ttgataatag gggttccggt catgtctgat ttaacatacg
     4261 ctttgattat ctttacgagg tcaccgggtt ttagactctc aattccagga agattagctt
     4321 tttcatccca taatttcact ttggctctag aatctgaatc ataaactgtt atggtacgaa
     4381 gaagaaactg tgtgccatct ttacgtgaaa attgtttagc tggataaaca ttcatcactc
     4441 ttgtctctag ggtgatttcc tttgctccaa cgtaaagatc tttcagaccc atctcaactt
     4501 ttagttgttc gtttaatgac acaccaagat ccgatgctat tagaaataat gccccttgat
     4561 ctgtcaagta ccctgcacca atcttttctt tctttttatt gatcatttcc tgtatgtccg
     4621 atctagaaag atctggtttt tgctcaagaa gtttgtttaa aagtaattcg aattctgaca
     4681 agtcactctg aaatagaagt gtgcattaat aaaaatttca ttaactaaat tagtgggaga
     4741 atcaaattcc atttaaatgt cctgtattga aacgaatcta ctatccaaat catatgattc
     4801 cacgatcgca gtggatcgca tagatctatc tgtctattcc ggtaagattt ttggattttt
     4861 gggaccaaat ggagctggaa aaactaccac tataaaactt cttacaacac tcatagctcc
     4921 aacaagtggt tctattgaca tacttggaat agatgcaata aggcaccctc ttgatgtacg
     4981 aaaaagaatt ggtgttgttt tacagcaacc cagctatgag ccaaccctta gcgtagaaaa
     5041 atctcttgaa aagtacggga tgatgtggaa tgttgagaga aaaacaagaa aagatagagt
     5101 ggaggagctt ctcttgtcat ttgctttaga agatataaga aatgtgaaaa atgaagatct
     5161 ttcaataggc cagaggagaa gagtacaggt agcacgcgag tttatgcatg atatggaatt
     5221 attgtttctt gatgaaccga cagttggatt agatccatct gcacgtagaa cactacttga
     5281 ttttatcaaa aaaagagtaa aggcgggtct aactgttttc tttaccactc acatactgca
     5341 tgaagcagaa tatctttgtg atgaaattgc tattataaac aaaggtaaga ttatagctat
     5401 agatactcca gaggatctaa aaaacaaatt cggtagggag aaaacaataa agattcacat
     5461 aaataacaca gaaaaaaaca tgtcaagtct tttagaagga atacaagact ataaacttga
     5521 ttttaactct ggaacaaaca tcacaatacg ttctaacaat tctgaagaaa tattattgaa
     5581 aatcctccaa atcttaactg cgaacaatat cacaatagat gatttatccg cggttccgac
     5641 aagtctagaa gaaatatttc tttcaatggt gaaaaaatca aatgcatcca ataattagac
     5701 ttgtaaatag aaacctgaca atatctctta acaaggggtt tttaatttgg caagtcattt
     5761 ttcctttgat ctatatcttc gttggaggat ttgcttatac atcattaatt gattctgtcc
     5821 catttgcaaa taaaacactg gattatccag cgtatatcgc atctggaatg attggtttta
     5881 acataatgaa tagcactctt gtctcaggca tcataatatg gaatgatcgt aggcatggaa
     5941 tgtttgagca gatccttgta ggaccattca ctcgtcgtca ttatattcta agtaatgtgt
     6001 acacaatagg aattgttggt ctaataagcg ccgctctaat tacaattgta ggctatccac
     6061 tatttttcaa ttctgtagaa tttaatctat ttacaattcc gcttttggtg tttgcttccg
     6121 ttgctggttc aattcttttt ggttctatag cctctatcat atctactaga ttgagatcaa
     6181 gtgagggatt caatgtcgtc attaatacgg tgtttttgtt ttttgcattc gtgagtactg
     6241 cattttaccc agctgaaggc gcgcctgcac caattgctac tgcattttat tttaacccac
     6301 ttacatatct cgtagatgta gttagggctg ggatctttgg aagctttgat acttttgtag
     6361 ctttggaaat gcttgttcta gtaatactat ctctaggctt gtttgctgta gcaacaaaac
     6421 ttcttgcaaa acttgaatta tgattataaa caagatcaat ttgatttaaa ttctagaaaa
     6481 caataccttg tttatggaag taagaaaacg aagtagtttt tggtttcttt taccgatttt
     6541 tttcaatgta atcggcggtg taattgctta ttttattatc aaagatgatg atccacgcaa
     6601 agcaaaaaat tgtctgttgc ttggaataat attaactgcg atttcgtttg caatttttgt
     6661 agtgccaata ctaattggaa tatcactcat gcctaatttt gatatgatgc caaatgagcc
     6721 atatctgtaa gtgattttaa aatagcccgg cccagattcg aactggggtc aagggctcca
     6781 aaggccccta tgcttgaccg ctacactcgg tggatatcct ctaccgggct ttccgggcgt
     6841 tgaatgttga ttttggttcc cttataatgt tattctgtat cagtaatttt ttaataatat
     6901 ggcactttaa caaaattcta tttacattaa aacctagatc gatttaatcg cagtgacaag
     6961 ctttggaaat ggatcatcca tggatttcaa tatctttttg gcattgttga tatctttttt
     7021 gttatctgat tttaagaatt gctttacaag ccgaactatt ttttcaggat ccttttctct
     7081 ctttaccaaa tgtttcttga caagatattg ttctatgatg tttggtactg catcatatga
     7141 tatagtgggg attcccatta atgcagattc agcagtcatt gtgccacctg atccaataaa
     7201 aacatccgtt aacatgagca gacctttccc atctacaact ttattcagta tggtgatttt
     7261 atctccaaat tcctttttga actgattaat ctggggggtg tatctgccca agatgactat
     7321 attacaatct ggaaatttat tggcaattct acgtactatg tgaaagctat ttttcctagt
     7381 agaaatatag gctgcttgcg attcctcggt tctgattact attgtttttt tattctttaa
     7441 tgaaaagtct ttttttgaat atttttttga tttgtttttt actattaaag atgcatcaag
     7501 cgatttgtat tgtatgatat ttctttgcga gataccatat tttgaaaatt ctttttttgg
     7561 tataatccaa ggtatgagca gtttttgtac cagtggtatg gaaagtctca tcaccgcttc
     7621 tgcgtgaggt gaatcacaga aagcaacatg cttgatgcca agaccatagg aaattcttgc
     7681 agcttcagga gagcaaaaac tcacagtaag attaggtgca aatttcctta ttcttgaggc
     7741 gaggccattc attcgtaaaa tactggcatt taacttgcca aatctatcat cgcctccatg
     7801 tttgcctata aacacgagat ttatgcctct gatttttgca agctcaacaa cctcacgata
     7861 ttttcttgaa gtacagagta tctgattgct tttttgtaac ttttcaatca tggattcaaa
     7921 aaacaaaagc tgttttggag taagtatgtc aaaccagatt ttcaaacaat tttgatagaa
     7981 ttctgaggtg aaaaagtttt tcttagcctt tctgcctagc tccaatgatt tgggaggagc
     8041 taaagttgtt gtttatggtc ttagtacgga aggttatgcg attgcttgtc aaatggcaat
     8101 taaaggtgca gacgtcttta tcgtagatga atctgcacat atggccatat ctctcaaatc
     8161 agaaattgca aagacatacc cagatgttgc ttcattgaag gaagacgaac cattacttgg
     8221 tgtagaacca atagaagttg cgatatctca ttctgaatat ctcttttttg ctccaagaat
     8281 aaggaaagct ggtcaggaaa taaaaatgga aattcattct aaatttaaag acgcaactag
     8341 tgctataaag aaaggcggat ctgtaattta taatctacca acaggtgttg gtggaaacaa
     8401 cgaaaccatc tcactcttag aacacgtaac aggacttgag gtaggaaaag gtatctcgta
     8461 tttctactat ccattaggta caaaaaatca agtacctgaa attataggat cttttaatgg
     8521 aaaagctgat gataagcttg ctaaattact tgaaactgat aaaaaagaaa aaaaattcgt
     8581 agcaataaca tctgcagaac tttttcatgc gataaaaatt ctttcacaat tttctggact
     8641 atcgagtata ctagaagtat gtaagtttgc acaagatgaa aacacaagaa atgatctgat
     8701 gtttgcagaa ttcaagaata tgttcttaga cgatatgacc aataatctct atgatctgcg
     8761 ttcccttgga acctcgcttg agggagctgg caccatgatg tacctagtca atgggagcat
     8821 aaagggtatt gatggatatg taaaacgtct aatagatgaa attcgttcaa cgctgaaaaa
     8881 aaatgatctt aaagcaagta gaacaagagt agcacttgtc tggacattaa atccatatga
     8941 gatgcggggt gacaaagttg acatgttgaa ttctcttgtt acaaaattac gtgattatat
     9001 tggtgatgtt gaaatccatc aaggcccaac cttcgatctt tttcacagtg ataaaaccac
     9061 aattgttgta gcatgttcaa aattagacca tgaaaagata aacaaaagtg gaaaaggctc
     9121 tgatctcata attataaaga caaatccgct ttgtgagata atgtaataag agtaaaatta
     9181 gcctaaagca tcaaacaact tatcttgtct gaagaaaatg atacaaaaac atctatccag
     9241 gtggaaacta gttctgacga tgttgaagaa tcaaatatgg atgaattaaa aggcaaactc
     9301 gctaggcaag aagaaaccat aacatcatat gagaaaaaac ttcaatacct tctcgcagat
     9361 tttgaaaatc taaaaaagag aacagaatca gatattcaaa accgtgtaaa ttctatattg
     9421 tctgaaattg tgctaaaatt tctatctatt tatgatgatt ttgtgcgtgc aaaagaagca
     9481 ctagcaaaac aaaatgtaaa cacagaagga ctagatgcaa ttttaaaaaa tatggattct
     9541 tttctattag aatatgatgt aactccaatt gatgcattag gagaaatatt cgatcctaaa
     9601 ttacatgaag ccataactgt aaaagaagac ccaacattgg atagtgacac aattactgca
     9661 gagattcgta agggatatat tttacagaat agagtaatta gaccaagctt agtagaaata
     9721 tcaaaaatat caaaaaataa cttggctggt gaaaataatg agtaaaatta taggaatcga
     9781 tttaggaaca agtaattctg ctgcatccgt tatgatgggt ggaaagccaa ccacaatacc
     9841 tgcagctgaa ggtaccacaa ttggaggaaa agcatttcca tccattgttg cctttacaaa
     9901 agacggtgaa cttcttgttg gagagccagc aagaaggcaa gctgtaacca atccaactgg
     9961 aacaattgtt gcagcaaaac gaaagatggg gaccgatcat attttcaaag taaatggaaa
    10021 agaatacaaa ccccaacaaa tttctgcctt tattttgcaa aaaataaaaa gagatgcaga
    10081 agcattcatt ggagaaaaaa ttgaaaaggc agtaatcacg gttccagcat actttaatga
    10141 taatcaaaga caagctacaa aagatgcagg acaaattgct ggacttgaag ttgttagaat
    10201 aataaacgaa ccaactgcgg cttcacttgc atttggtctt gataaagcaa aacaagacat
    10261 gaaaattatg gtatttgact ttggtggcgg aacactagat gtcactataa tggaaatggg
    10321 aggaggtgtt tttgaggtca tgagtacatc aggtgataca caacttggcg gaactgatat
    10381 ggacaaagtt gtgatagatt acatacttga aactttcaaa aaaagttctg gaattgatct
    10441 ctcaaaagat aatactgcca tggttcgagt aagagaatct gcagaaaaag caaagataga
    10501 actttcttct gtaactgaaa ctgaaatcaa cctaccattc gtatcttatg atcagacaac
    10561 tgggccaaag aacctagaga tgaaattaac aagagcaaaa cttgaggaac tcatcaatcc
    10621 aatagtggaa agatgtagat catcaatgac aaaatctttg gaagatgcaa aactatctac
    10681 taatgacatt tcaaaaattg ttctcgttgg aggacctaca cgaattcctc tagttagaaa
    10741 atttgttgcg tctgtaatgg gcaaagaacc agaatctggg gtagacccaa tggaagcagt
    10801 ggctctaggt gctggtattc aagcaggaat catagctggt gatgttacaa gtgatatagt
    10861 gcttcttgat gttactccgc ttactcttgg agtagaggta cttggaggat tacgtgaacc
    10921 acttattgac agaaacacca cgatcccaac ttcaaaaggc aaagttttca ccaccgccgc
    10981 agataatcaa actgcagtta caatacatat agtacaaggc gaaagaccga tggcaacaga
    11041 caatgtttca cttggaagtt tcaacctttc tggaattcct cctgcaccta gaggaattcc
    11101 acaaattgaa gtaaaatttg atcttgatgc aaatggaata ctgaatgtag tagcaaaaga
    11161 tctaggaaca caaaaggaag ctaagatcac aataactgca agcacaaagc tttccaaaga
    11221 agaaattgag aaactaaaaa aagatgcgga actatacgct gaacaagata aaaagaaaaa
    11281 agaagaaatt gatgtcaaga atgaagcaga aggatatatc tatacaactg aaaaattagt
    11341 aaatcaggat cttaaagaca agataaaacc agaacaagta acaaaagtaa atgatgctgt
    11401 aaaggaatta aaagatgtac ttgaaaaagg ctctatagaa ataaagccaa aacttgatgc
    11461 gctaaaatct ttagtgaatg agatcagtac agaactctac aaaaatgtca ctcctccgcc
    11521 tggatctggt caacaaactg gtgcagaaca acaagcaggt aatcccaacg aacagaatac
    11581 cagtcaaagc agcacgccat aatctattga tagtttatac tacccatttt cattaattat
    11641 ttttgaatca tgtcaaattc gattaatttc ttagctattg ttttttcaat ttttcttatt
    11701 gcaagtacgc aaaattattc tgcctcggca gaatctgata ccatcaagtt ctatggtatg
    11761 ggtaaggtag accaatcaag tcagttttcg ggtgaaattg taagaacgat aattgatggt
    11821 gacaaaggga caattataca cagcacagac aatggtctag ttgtagtaag actagaatta
    11881 tcagaaagtg aagcgtgtat tctaaaacta tcgactatat gctttgacgc taaggtaacc
    11941 aaagtgaggg gaaccgatat tcaggttggt gatgaaatta atctaactat agacctagaa
    12001 aacaaaaaag aattcctctc tgttgtttca ggacaattcc aaggcgaaaa tatcgtaatt
    12061 gatcttgtaa agactagaac tagagataat ggcgaattta ccttaatgtt tacacgtgaa
    12121 ggaggaattg ctggaatgca gagaaaaata acaattgatt cagaaacaaa aacaatgcta
    12181 attcaaaatg gtcctgacga accttcgcca ttagttctta gtgatgatga tattacacaa
    12241 ataagagata caattcaacg aaacggcttt tttgatttta atataaaaaa atatcctcca
    12301 gttgtaggat cagcagatta ttttacttat gatatggaag taaccgctgc tcattttact
    12361 aacaaaatac aatggactga tacttctgaa aatgttccaa aaaaacttta tttaataaat
    12421 gaaaaactac aaaacattat tctatctttc atagacgaac atccattaga aattcttcca
    12481 cttaacattg cacgtgaatt tgttatttct agtcctactt ttgcttttga tggtatggaa
    12541 gaaaccttac aagtacaatt aagcagcgta actgatgaag ccactcctga atacgaaatt
    12601 cacgcaaact ttgattcaag acatggaggt tatggagaca gaacaggtca gatggttaca
    12661 gaagcaatca ctccacatct catggttata acaatctcag atggtaaggt tgtatctgct
    12721 atcatagacg gtacatggga cgaaattaat caaaagttac tgtagctatg agtgctaaac
    12781 gagattatta tgaagttctt ggcctgcaaa agaatgcttc agaggcagaa ataaaatcgc
    12841 aatatagaaa actagctctg aaatttcatc cggatagaaa caaatctcct gatgcaacag
    12901 aacattttaa agaaatatca gaagcctatg caattttatc tgatggtgaa aaacgcaagg
    12961 tatacgatca atatggtcat gctggtgtag atggaaggta tagcactgag gacatattcc
    13021 gtggtgcccg agttaacttt gaagatattt ttggtgggtt tggaagtggt ggatttgatt
    13081 ccatctttga atcacttttt ggtaggggtg gtggttttgg aggatttggt agagaaagag
    13141 gagcagatct gatctatgaa acttcgataa cactagaaga tgttctaaag gggaaacgag
    13201 aagaaattga tctacaaaag gatgtagaat gtgaaaattg taaaggttca ggttgtgcac
    13261 ctggtactac aaaacgaaca tgtactgtgt gtaacgggca aggacaaatg aggactacac
    13321 gtaatatggg attctcaacc tttgttactg tacatccatg taatacttgc aatgggcaag
    13381 ggaaaattat agaaagacca tgcagcaaat gcaaaggaaa aggcaaacaa aaaggaacaa
    13441 aacatctttc ctttgacatt cctccaggcg tagatagcgg cgaatatact attcgtggag
    13501 agggagaatc ggtagaaagc ggaataaacg gagatcttgt tgttagaatt agagtaaaac
    13561 cacatgagaa attcaaaaga gacggggctg acatattcta tgacgctaac atatctatag
    13621 ttgatgcagc tcttggcaaa actattacag tccctactct ggaaggcaca gaaaagatga
    13681 ctatagagca gggtagtcag ccaaacacga taataaaact caggggtaaa ggattaccac
    13741 atgtgaatgg taggggaaaa ggcgatgaat atgttagact tgtaatcgta ataccaacca
    13801 agcttgacaa gcaccaaaag aaactactag aagaatttcg agatactctt aattaataaa
    13861 aaacacaagt gaaaaagttg agaatagggc ttgatgttga tggagttctt gcagacgtta
    13921 ttcaagcatg gctttcttac aataataata ttcgttccta tatctcaaaa tcagatatca
    13981 ccgagtggga tttctggaaa aaatttcaaa tagataaatt tgatttttat aaagaactct
    14041 ctctgtgttg gaaatcttgg gaaaacattc cttctacaga agaaagtctt tctatagtaa
    14101 cctctgaact ttcaaaattt ggtacggtgg acatagttac tgcaagagaa gaatcaacac
    14161 atgatcatgt aaaaaattgg cttaaattaa aaaaaattaa ttataaaaat tatgttggtg
    14221 tttttgaggg aacagaaaaa actaaactag actatgatgt tttcatcgat gactcaccaa
    14281 taaatgcaaa aagtatgctt tgtgaaggaa aatcagtaat actttacact caaccttgga
    14341 atctgagatt tgatgataga agagctcaaa gaatacatca actaaaagat gcaattccta
    14401 taataaagac acttattcag gaaggctaat ttttttcgtt gcgggggtcg cctagcctgg
    14461 tcaacggcgt aaggctcaga accttatctc ttaggagttc gtgggttcaa atcccacctc
    14521 ccgcatgttt gtaaataaca taatacatgt ctaaatacgc caacaacttt aaacctcagt
    14581 taaaagtaag caccataatg ggaaaaatca aaagagtgag tggccgtggt acttcagttc
    14641 gtgaagaaga tgaaaaaccc cagaattggg caggagatgc atatagagat gttcaaaaaa
    14701 agaaaatgga atcattaggg aaagacttca aaatttctag cggacgtggt actggaagca
    14761 agaaactcca agatgcaccg gaaaaaccat ctactaaggg ctattatgtt agtactggta
    14821 gaggcacagg aaaaagaaaa ctcgaataac tgttcacgtg attacatagt aaaaaatcag
    14881 tgaactcttt attttagcta tatttatttt tagatttata gaaaatttat aatgctaatt
    14941 taaaagtcaa attaattatt tttgacgaat tttttcttta attttctttt ccaaaatttg
    15001 atttaatttc tgtccatcta cttttccacg cattgttttc attgcaattc ccattaacgg
    15061 accgattgat ctttcacctt gttctttaat aatgccaata tttgcattaa caagatcttc
    15121 taatgcattc tcaatctcca tgggttcaat cttttttatg gctgattttt caatagtttc
    15181 aacagctgaa tgtgtcttac ctgacatgat gctttcaaaa atcatctcaa tggattcttt
    15241 tgtaatttta tcttctgcaa gaaagctaaa aatttgaaga atttcagcag gttttaacag
    15301 ttctgcattc aatccctgcc tctctaggtt tgtgatagta ctgcaaagaa tggatgcagc
    15361 aaaatttggt gaaattctct tatcctgaca aattttctca aacaaatctg aataatttga
    15421 atcaaaaatt tgtattgcaa gctgtgaatt aagctgatat gttttttgca gatctgatat
    15481 tgtctcttcc caagaatgcg gtatgttttt ttgtgcatct acaagttcat tttttgtaac
    15541 agaaatgggc ggaatgtcag tttctggata catccttgat gctccaggtc ttggtctcaa
    15601 atacacagtt tcaccattaa cggttgctgc tctagtttct gctggcaccc ctatttttgc
    15661 atcttcgatt cttttgataa ttgattctat tgcaggatct acctttcgta tttctcctgc
    15721 taatattaga aatgcatcat tttgttcaag ctccaacaat tttctaacct cctgaatctc
    15781 aaaattctct attccataat ttggtaactc atcagaatga aaaaccccac ctaatccaaa
    15841 aaatcttaca agttcaccaa tctgttttcc caatcttatt ccttcatatg gttcaaaact
    15901 aaacatacca gagagatttt ttattctaat tgcctttaca actgcgcctt ctgttaatga
    15961 tttttgtata gtttttgatt tacaattttt gaaaatcttt gtaacatcct ttacatctct
    16021 ttcctttgct atcctatcag tttctatttt ttttaattta tccgcaatta tgagtaagcc
    16081 atgttgcctt ttcgcttcat attctatgac tttttcaagt tgatctagtt gctggacacc
    16141 ttttacttca attatatttc caccctcgat ggatacattg acatcttgtc ttattgttcc
    16201 taaaccacgt tccactcttt ttgtggcacg aagtaatctt ccaagggtta gtgcaatttt
    16261 tttaatttct tcaggtttac cagaaattgg ttctaacgca atctctacca atggaacacc
    16321 aagtctatcc aaagaatatt cttttgtttc tatagtttca cctaggatct ttgctgcatc
    16381 ttcctctaaa caaatgcctt gtactccaac tttaattcca tctacttcaa gatggcctcc
    16441 ttgtgaaaca agcatagttc tctgaaaacc agatgtatta gatccatcta tgacaagctt
    16501 tctcatcaca tgaatctcat tgaaaatatt tgattttaga gcagatgcta taacgagcac
    16561 aatttctttt gcatctttat ttacaccatg aggaggttct tcgtcttgtt ccacaaggca
    16621 actacttgaa ggatttgcat gataaactat gatctttgcc ttactacgct caaatacggc
    16681 tgtaggatca tattcgccaa gctcactctt agcaagtctt aacctgcgaa aaaactttat
    16741 cggatattct tctgattctg tattagaaca gttacaaaat aattttttat ttgtagctaa
    16801 ttgctgatgt atttctaaac caacttttaa tcctatttta tctaactgaa gattagacat
    16861 ttctcattta ttatttgtct tactttttaa gcttagttag tctgaaaatt ctagggctat
    16921 gttttctctc atcaattttt ccatttcttg atgattcttt gaattgccaa gagcccacat
    16981 tgttttgaca agtgcagtct caggaaccat gtttgcgaga ggaattactc ccaaactcag
    17041 taaatccctt ccactttcat aaactgtcat gcttaccatt ccatctatgc attgagatgt
    17101 catcccaata aacagccctt tcttctttgc tttactcacg ctatcgtaca taacttttcc
    17161 aacatgccca agacctgttc cttcaaaaat tattgcttta tgcccatctt ctattatgtg
    17221 atctataatt tttggactaa gacctggata atacttcact aacgctacac gtgtatcgaa
    17281 attaattctt ggtaggtagt cttttttagt gtaattgaat tgtgggtttt tttctatctt
    17341 gtcttggttt actataaacg cggggtttcc tcccacagtc tgaaacgcat ttcgcttgct
    17401 agtgtgattc ttccttactc gcgtacccca atgacaagca atttcatcat cacttgtggt
    17461 attatgcatt accacgtaaa ttccaccgat attgcattca ataaggaatt ttgttgcagc
    17521 aattagatta gtagcggcat cagaagatgg tctatctgat gatctttgtg atccaactag
    17581 cgcaatagga attggaatac ctgctaaagc aaaagaaagt gcagatgcag tatattgcat
    17641 agtatctgtg ccatgagcaa ctatgattcc tatgtattcc gatttggtgt acgagtttat
    17701 tttttctgct attttcttcc agtgttctgg tagtaaattt tcagaatatt ctgaaaaaag
    17761 aacttctgtg tctatgtttg ctatgtttga aatctccggt acattcgcat taagttcagc
    17821 agctgtgaga acaggtgtga ccccacctgt tctgtaatct attttacttg caattgttcc
    17881 acctgtagaa attaagagaa tctttggtaa gtttggatta gtttgtgatt gaggaatagt
    17941 ttcttttttt attgaactag atgatgattc tacttcaact ttctttattt tgtttatttc
    18001 aagcccaata ttatatccac ttttaagctt taaaacaata tgctcatcat cactgtattc
    18061 ataacgtggc atgacaatgc cagaatattt tgaatcagta actactatga tcaaatcgcc
    18121 tacgctgatg ttgttttttt gtaggaaatt gagagttttt cccttgtatc cgctgtgact
    18181 tgacatttaa ctgaattaga actgttattt tattaaaatt acctgtagta gggaactgcc
    18241 actttttcgc ctatcttgag ctctttttgg gttcttactt ttctgactgc ttcctcgaaa
    18301 tgtctcattg ttactttggc agttgcagcc tgtttttcag cttctttttg atctggatat
    18361 ttatcaagaa attcatgtat gactagtgat actgcagtat ttgcaataga tgccacatca
    18421 gctccactta gtccatctgt tgtatctgca attttttcta aatcaacata attgaggttt
    18481 tttccagcct ctgttttttg ttctctatct attggaatct cctttgtact aatttccaat
    18541 atcttctttc taccttcctt gtctggcata ggtacaagta ttattttgtc aaatctgcca
    18601 ggtcttagta atgctgtatc gatcatatcg gctctattgg ttgctgcaag tacaactact
    18661 ccatgaagac tttctatgcc gtcaagctct gttaagagtt ggcttaccac tctctcagta
    18721 actgctgttt ctccaccgac tcctctcact ggagcaatag aatctatttc atcgaagaat
    18781 attacacatg gggatgcttg tcgtgccctt ctgaatattt ctcgtattcc tcgttctgat
    18841 tctcctaccc attttgataa gagctctggt cctctaacag aaataaaatt ggcttcactc
    18901 tctgttgcta ctgcttttgc taaaagtgtc tttccagttc cacttggtcc atgtaacaaa
    18961 attcctcttg gcattctgtg accaagttta gagtaaagtg taggatattt cattggccac
    19021 tctacacctt cttgtagttc gcgtttgaca gtttctaatc ctccaacttc atcccagctt
    19081 acatctggat tttcaatgaa tacttcacgc attcctgacg gtgttacttc ttttagcgca
    19141 ttctggtaat catcataatt tacaataagc ttgtctaatg tttcaggtgg aagcttttca
    19201 tcttctaagt taagctctgg taagagtctc cttaagcact tcattgcagc ttctttgcaa
    19261 agatattcca aatcggcacc tacataacca tggctaattg ctgcaatctt ctccaagttc
    19321 acatcatcac ttagaggcat gtttcgtgtg tgaatcatga ggatgtcctt tcttcctttc
    19381 ttgtctggta ctttgatctc aatttctcta tcaaatcttc caggtcttct tagcgcagga
    19441 tctattgcat ttggtctgtt tgttgcagat attacaatta ctttgcctct tgcttccaaa
    19501 ccatccatga gtgatagtag ctgagaaacc actcttctct caacctctcc tgtcacctct
    19561 tctctttttg gtgcaatgga gtctatctca tctatgaaaa tgattgaagg cgatttttct
    19621 tttgcttctt taaaaatttc tcttaacctt gcttcgcttt ctccatagaa cttgctcatg
    19681 atctcaggac ctgaaatgct gataaaatgt gcattacttt catttgcaac tgcttttgct
    19741 agcaaagtct ttccagtacc aggtggtcca tagagcaaca caccttttgg agcttcgatt
    19801 ccaagttttt caaaaatttc tggatgtctt agaggaagct caatcatttc tctaactttt
    19861 ttaatttcat cagttaaacc accaatgtct tcataagtga cctgtggaac tccacgtaag
    19921 gtttcacctt tttctgcaat gtgaaatact gttttttgtg taacaagtac tgcatctgca
    19981 gctggcgtga ctccaatcac ttggaaagtt aatcttccac caaagtatgg caccatgaca
    20041 ttatcgcctt ttatcaatgg gacactttct agtgcatccg caagatatct ttcgtctatg
    20101 ggtggaattg cttcaagtgg tgctactaca accttttctg cggcaactgc cttgatcttt
    20161 cgcactgcga cagtatctcc gatggctatg cctgcattat ttctaacaag tccgtcgact
    20221 cgaataattc cttttccttc atctgatggg taaagcggta aacattttgc tacggttctt
    20281 ctttttcctt tgatctcgat aacatctcca gttgacgcac taagtgaatc catggaatca
    20341 taatcaatgc gtgctactcc tctcccgaca tcacgagtat atgcctcgag tactttaaga
    20401 gaaagagtat tctggctcat agaataaaca ctcctaaact aatttttata cctttgttgg
    20461 ctttgatcca ttacatacag tgatccaaag cttaaaatca acaagataaa acagctcatc
    20521 tgctttgggt aaaagaccgt tagttagaag acgtggacga ggtggaatgc aatttcgtgc
    20581 agcatctact ggtaagattc ttccagcaaa atacccaaga tttggacttg cagaacagcg
    20641 cgaaggtcaa gttgtagatc tggttcatga aagaggtaga gacactcctc ttgcaaaggt
    20701 cagatttgaa aatggctcat tttcattttt accagcaatt cttggcacca gaattggctc
    20761 aaaaatccag tttggattaa aatctgaaat tgctccagga aatgtaatta gtgttcaaaa
    20821 cattcctgat ggaacaatag taagtaatgt tgaaaaacac tttggcgacg gaggaactat
    20881 agttaaatct gcaggaacat acgctacagt gttttcacat agtgcagatg gtgttacaat
    20941 aaaactccca tcgggaaaat ttactactct taatcctcag aaccgtgcaa tgattggtac
    21001 tctagctggc ggtggtgtag gtgatagacc attcatgcga gcaggaaatg cttggcgcag
    21061 aaaagtatcc aaaggtcaca agtacccaat cgttagggga gttgctcaag ccgtatatgt
    21121 tcacccacac ggaggtggaa gacatcaaca cgtaggtaga agttcaacag tgtcaagaaa
    21181 tgctcctcct ggacgaaaag tgggtagcat tgctgcaaga aagactggcc gtgcaagagt
    21241 taaagataga ccagattcca gctcttctta atattttcaa cactcaagac taattaatcc
    21301 tggaaaaaat tctctcatgt ggcaaatcag agagtgcata tttttgtaaa aggcaaagtt
    21361 cagggagttt tttttcgcca aacaaccaaa aacgtagcag aaaagaaaaa tgtcacgggt
    21421 tgggtaagga acctaaaaga tggaagagta gaggcagttc tagaaggtca ggacataaac
    21481 gtaagcgaag tagtagaatg gggtcatagg ggtcctccaa atgctgtagt tgaagatgta
    21541 caaatcatta atgaaaaata caaaggcgaa ttttctaagt ttgaaatact atactagtga
    21601 atagttttgt atgcgtctga aagtaaattc tttatttcat taagatcttg acttggtttt
    21661 atttcgatct ccttttgagg ttcctttcta tctcttctta tttttattaa aatagaatcc
    21721 ttttgtggtt ccatatctgc aaagaatctg aaagtcatag atttacaaaa taccactctg
    21781 tgcatacgca catcttcaac tacattttct ccaagagaaa aacaatactc tctaagagaa
    21841 tcaaacagtg gttttatcaa aggtgtaagt tgtttttgga aatcttgata gtttctgttg
    21901 ttacctgaaa aaatcaaatc tcccatatct tcaaattaat ttaagaatat atttagtagt
    21961 agctccacca gttccagtct agacaaatag ccccatctca agtcaagcaa gatccgccac
    22021 gtagacgacg cagcgtgaat gttctgcctt aggattgctc cctcccggac ctcatccctt
    22081 tcagcagctc ggtcttgaag accgtccctt cggacagctc cagtcctgca tccacccgct
    22141 tcggcgtgat ttcatttccg caagtcaagc aggtactatc catctaaaga tttacccagc
    22201 agttactgat ctctgcatat agccggtttc agatttgggg cacggctagc gccccgatcc
    22261 tccctactac cagtatatgt tacaaagaaa tcttatattt gcattaggat ctgtcttgct
    22321 ttagatttac aagcatgtca catacggaac aaatctcacc agtacattca ttcccgcatt
    22381 ttgggcatgt tcttctttgc ttatagtttg tgtctttaat tatgtgagaa attcttgtga
    22441 tagatctatg catgttgttt ttgactccgc tgtgtttctt ttctaaagaa tttagaaatt
    22501 cacggatctc tgttctaatc ccctcattca tgtgtggaca ctgttctgat tggaaaggaa
    22561 tgtcatttgt gaaagcataa aacgcaatct cattttcata aatctcacaa aatggcttta
    22621 tctttctgat cttatttgtg gatgtgtctg gatccatcca ccctattttg tttgtgtctc
    22681 cagacagaac attgatgata aaagtttgaa gaacatcatc taaattatga ccagttgcta
    22741 ttacatccac gtctagatct tttgaaccaa tatcgattgc acgtcttctc aacgtaccac
    22801 aaatcgaaca tgatgttgtt ttttcgtttt ctctgagctc aagtgattcc tgtaaagtca
    22861 tatcaaaaag atctttgtat gcataagatc tgtgttcaac tcctaaacta ctgcaaaaat
    22921 tctttacaat ttgtaatgct tcatccctat atccaggaat gccttcatct accgttattg
    22981 ctctaagtgt aaagttgtgt gattctgaca ttttcttgag aacatgtaga agcgataaag
    23041 aatcctttcc gccagatact gctacaccta caacttctcc attctgtatc atgttgtatt
    23101 tagatatgat tctggctgtt ttttgtaaaa tggaatttga aaaacatgta gaacagagat
    23161 tctcgccaga atattttctg gaatatgctg caacgttatc acatctatcg catttcatag
    23221 agggtttttt ttatgcctaa atttaaccat atgtgtattc ttgtaaacta ggttttgatt
    23281 ttaaattgta acaaaaccag attttattgc tgaaaatgca atgtttagac caaatatggc
    23341 aaacgtgaac gcgtaaatgg atagcatggt tactttcagc actttgtctg aaattttttt
    23401 ctcaattatg ctttgaatga atttcccccc atccaatatt gggaggggaa gcatgttgaa
    23461 tatgccaatg aaaaatgaga gcatccatag ccaaagcaaa aacatgaaaa cttcaggact
    23521 ccatggtatg taatatggta cagttggctg gtaagccgga aatgtgcctc ttgtaattcc
    23581 aagtaatcct ttttgtggat catctggcgc ggctttagtt atgacaggta tctgcattgt
    23641 ctcgcctttt ctaattagtg taactattgc agtttctcca ggtgcaagtt ttactttggc
    23701 aaaatcttgt ggttgaatta ctgaaacgcc attgattgca gtaatgatat catcttttaa
    23761 tagtcctgcc atagcagcac cagaaccttc agttacagaa agtatgggaa cccccaatgg
    23821 atctttgtaa aatacatcgc ggattggttc tggcattaat agggcaaaag caggattgaa
    23881 aattaacaga cctgctatta tgaatgaaaa aaggacgttt gatgttgcac cagcacctat
    23941 cactctgagt ttagaaattt tttttgcttt tgcaaattct tcctcatcgg gttctacaaa
    24001 tcctgcaaac agagctataa acactgcaaa tcctcctgtc ttgatcttaa ttttttctag
    24061 tgtggcaaca attccatgag caccttcatg tatgaccaga acaatcggaa ctgttaacaa
    24121 aaaatatgcg atgtttattc cagattgaat tgtgactcct ggtatcaaaa ccgtcatttc
    24181 agaaaacgat tctggtttga caaagaaatt tgatagattg gagataagaa accaaaatgc
    24241 aaaacccatc ataacaaatc ccgcaataac actcgtgttt gcaaatatct tggtggcctt
    24301 ttcggttttg cctaaaagtc tggttagagc aagctgcact ttgtaatttt tgtacgtaag
    24361 actgtatggt tttatgtcaa aaccatgttt gtcaagcttt gaagcctttg ctagtatgat
    24421 tactaccacc catgcaatta gaacgtagaa aatggaattc tgcgttaaaa aatcaattgc
    24481 caaattaatt gatactcttt ttagtcaggg caaatttatc agttgctatg gttgcaattg
    24541 taataatttc tacgtatcca aacaagaaat ctattcttaa aatcgcaaat tgtgtggtca
    24601 aaaaacatct tgcggcgtgt gtaaatttta caaaaataga ttcaatctat atctggcaaa
    24661 ataaacttga aaatacgaaa gaattcctgg cattattcaa aacaacaaat acaaaaaaag
    24721 aaatactaaa aaaggaaata aaaaaactgc atccctatca agttccagaa atcatggaac
    24781 tcaaagtaaa ctctgtcaat aaatcatatc ttggttggtt agtaaattca actagggaca
    24841 aaacgtaaaa gtgaaacaat tccaccaagt gatgacactc ttaatcctac gtctgttgta
    24901 gaatctactg aaaatatttc aacacctctg ctttcagctt ggttcaagaa ttctatgact
    24961 tgatcctcat cttgtttttc aaaaattttg tcagaaaaaa taagcgattc tactgcacct
    25021 gcatcatttg cctttgttgt ttcattatac cccatagtga atttgttgct ttttttgtta
    25081 accatgagca ttatctcatc gatgattttt gcgactttag caatcttgct ttgtgccatg
    25141 acttgtttca tagtttcaga ttttacgaaa gtgtaaattc catcttctcc acctgaatca
    25201 atcccttcaa caacctgaat cttgtgtttt ttacctgtag gagtttcagc aagataattt
    25261 cctaatttct tttttgtttc ccctggtcca aatattacta ttaaatcgcc ttcccgtaca
    25321 gacgacgaaa tggcattttc tacatcagca aaaaatgttt caatattaaa ttttgatttg
    25381 tatctttttc cacctgctcc agaatacatg ttaggcatta atttgaggtg tgttccactg
    25441 agtcgtccta ttccacaatc agctgtatct atggcaacca aaacaaaact atgaccttcc
    25501 tttttttcaa tgatcagttt tttttcaatg ttcgtccaat tcttttttgt taacgtaaca
    25561 acttcgccta tcttaagtgt caaggaatga tgagatcctt tctgtactga ttcactatca
    25621 gattctataa tggtaccatg aacctttaat ctatccacaa cgtcatcaat agaaattttt
    25681 tcaacattta gcgcaactcg tattttgatc ctctcccctc tatctggtct ggaaaagtcc
    25741 ttttcttgtt tcattgcgcg tgtagtgtct ccaacaaccc tatctccttt ttttatgatt
    25801 ctacgtaagg tgaatagatc atccggctct tctgctatca atgcaaccga acattcatca
    25861 attgttttag tgatcatttg tcatctttat gtaataaaac aagaataaaa aagaaaatag
    25921 atttagtttg ttaactcact ggctttggtc tgtttttctc cagccttttt tcttagatag
    25981 ttctgaaccc actcttgggt tatgactcca gcttccgtga ggtttacaag cgtgtttgaa
    26041 gaggcttctc ccgtgacctt gccactattt cgcctaatct gtctccactt taagttggta
    26101 tttccgcttt ttgaagaata tatgatgcca aagacatctt ttgcattcac aaatgtcatg
    26161 tcgcgaattt tttttagagt gaccttgtct gcagcttcta cataaataag acctggactc
    26221 tcttcacgtt ctggaaatac ttcataatcc tcaaagtagc tctctgggat gaaagagtta
    26281 caggtgctaa ggattacaaa cttcctgaaa ccttcaagtg aacacgtagc gtcgattgta
    26341 accatttgag ttaaattttg acctttgcct tttatcaagc ttcttgaaag actcaattag
    26401 gaaccataaa aattagatgt tgcaggcgtt gatgaccaaa tccctttgat ttgatgagaa
    26461 ggatcttgag ttctgagcct gcacctgtaa atatctatga tatatgatag aactactaat
    26521 ctaatgtaca cattgacaat ccacaagttt tgtatcaaac gtacaatcat gtggaccttc
    26581 tgacattgca tctataggaa tttttttcat acattcacca tgtcgtccct ttttacaaaa
    26641 ccaacaaact tggtctattt tatcattatg agaattgcat tccatgttac aacatcattt
    26701 taaaaaactg ttacatcacc aggagcagga gaatggtcat gtttattctt atgatagtcg
    26761 ataaaatctt tatgtgatgt ttcttgatgc ttacgtaatt cctccatact ttcaaacact
    26821 ccttcacata gatagcattt aggtttgtgc tgatctacta ttgataacac catcagtact
    26881 acattacgaa tttgttgtaa ttgaatcttt catgacattg atttgcttgt aattctagat
    26941 tggtgtaagc cactctgaaa actgttcgtc ttttcctaat gccacatcca tgaatgcgcc
    27001 ttgtagtttt ctagttattt taccgggctt accatttcct atagttacat tatcgatttc
    27061 tgtaactgat ttgacttctg cagctgttcc agtcataaac acctcgtctg caatgtatag
    27121 ttcgtctctg tctatgtttc tttcagttat cgatattcca tttgatcttg ctatttttat
    27181 tacactgtct ctggttattc catttagtat tccagcactt aaaggaggag tagaaatttc
    27241 ttcatcgttt accaaaaaga tgttttcagc gcttccttct gctaccttac catgatagtt
    27301 tagcattatg gattcatcat atccatcttt tagtgcctct actctggcaa gtgcagcatt
    27361 ggcataattt gaagccgcct ttgcctgcat gggctgagat ctagaatcga ttcttaacca
    27421 actagaaact ttgcatcgtg caccacgtac tttacctgca cttccctctc caagcttcca
    27481 ttcccaacac gctattgcaa catcaattct gtttggagtt ggtgttaatc ctagcacccc
    27541 ataactgtaa tatgcaatag gtctgatgta gcattctctt agtttgcttg cctttactgt
    27601 ttttattatt gcattagaga tttgttgttt agagtatggg atcttcattg aatacatttt
    27661 tgctgatctg aatagtctgt ctacatgttc tggtaaacga aatatcattg agccctttgg
    27721 ggtattgtaa catctgattc cctcaaagac tgcggtagaa taatgtaatg catgagtaag
    27781 aacatgcact tttgcatcct taaatggcac aagcttacca ttcatccaaa tcttgccttg
    27841 ttctttcata acaaaagtca ggaagttttg gtaatttaaa gaattaatct ttctaattgg
    27901 cgtgtattat acaagaatac tgtattttca aatgaaaaca tcttagacat actaatattg
    27961 catgtatgtt gaaactgagt tatggaacaa gtgttatctt ttgtagccgt aggattgcta
    28021 gtatttggtt tgataggtca agggtttgaa ctgcgaaaaa tgagaattgc tgctttgagt
    28081 gatggaaaca tctcatcttc aaacatgttc acaaataaaa aaaacctgaa atggtacatc
    28141 ataattagcg ctggtctgat aatttggtac gtctctggtg gctttactag ataatctata
    28201 tagatcaggt ctaaatatca ccgacaccta ttacatgata gcgcgtgttt tgggtaacgc
    28261 cgagctgatt ctagataaga ggctcaagaa caaacccact ttgacttcta taggtaaagg
    28321 cctgtagggg tcaactgatt ctgataattt ttaatctata cgaacttgat ttttgatctt
    28381 tctgcatgta ctgtacaaat cgctttttct atattatctg aaacatcttc aatcactatg
    28441 atgattcgtg aagtttcttc ttgtgcatcc atatttagaa tgttcaagcc tgcatcacca
    28501 atctttgagc tggcctttga agcaatttgt tgtacacgcc acatttcatc acctatcaaa
    28561 gtgattactc ctctgttata ggtaatagta gctaatgaat cgaatgcaag tacgtatctc
    28621 tcatttcttt taatataatc tgcatccaga aacagaattc tcgtaaattc aacatcgtcc
    28681 tttgtaaacg gtgacaatac tacaaattca ctgtatcttt tttcattttc aagtgactcg
    28741 aacaattttt gtgcggcggt actttctatt cttaaaatag cacagttttt cttacctgtg
    28801 actattttta gtggatgacc gtttttactc ttgggttttc tctcaatcgt tgttgttttt
    28861 ttaggattgt tcatgtctgt tattattatt ggtatgtcta tgccattttc tacaatttcc
    28921 ttgatagcta taggatccag tattttcatg ccaaacattc cagcgattct tgcttcatta
    28981 taggacagag tatctatctc atctaattcc tcttttacta ttcttggatc tgcagaaagt
    29041 acagagttat ccttctcaaa gtctattttt gtattgtact ttttagaaaa taaaatgcct
    29101 aaatctgcag ccgttctatc agaacctccc ctctcatagg tcgtttccaa cccatctgtt
    29161 gttttaccaa tgaatccccc aattgataat acgtcatttt tttcaagtag tgcttctact
    29221 ccgttcatac tttgggcaga gtgggtggca agaaagtttg cagattctat attcttgtct
    29281 gttattatgg gccatatgtc aaatcctact gcctctgatt ttatattgga actttttaga
    29341 acataatcca taacatacga catcaggatt tctccggagt aagcaagtgt acttgatctt
    29401 attacgtctg agaattcttt cttttctgca gcttgtttga tagatttaga tgccttttcc
    29461 aagaatgagt cgattgtttt tttacattta tcttgatgct ctttatttac aaattccaaa
    29521 attttctgat atggtttttt taattcatcc atatttggaa tttgacccgt ctgtgctttt
    29581 tgacctatca tcaatgcaat atctgtaaga gatctttgtt tgccttcata caatgtgagt
    29641 ggtgcagaaa aaacagcaat tacctttgaa tctgacttta attggtttat tctcttaact
    29701 atgaccggaa tcatttttcc gtctattcct attgcactgc ctccaaactt ggcaactact
    29761 aggtttttca aaacttgatt aaaatctcaa aatccttgta taaagcattg gatgaaaatt
    29821 tgaccaaata attcacatat gatgtaattt caaagaatat ctgagatttt actctcatgt
    29881 acatgtaatg atttctacgc attctttaat atgttgaatg tcaaaataag cgcatgagga
    29941 ttattatgcc cctacgaggt gcgacaattg aaggaaattg aaaaaggcga acttttcaaa
    30001 gacaataaca caaatcacat actgcaatat gttcctggcg ttgggttagt gcataacgag
    30061 actctgtgta aagaaattac gggctgtatt aatttccaac aaaataactg aaagtcacaa
    30121 taatgctttt atcagatccg ctgttttctg tgcaccatca aacatttgtg gatctctttt
    30181 ttcattcaat ttatttggaa ttagagattc aagatggtcc aaatcttcaa atgaaaaacc
    30241 aatttgtttt gcattttctt cttgttcaaa atgacctttt attggaataa aaataccagg
    30301 agttccaaaa tattttgctt catctattgt tgattttcct gcaagtgaaa ttatcaaatc
    30361 tgatgcataa ataaactcat gtagattatt gacaaaacca aggttccgta tttggctgtc
    30421 tgaaattttc aaagaagggc cagaaacaat gaacatgtcc gcatcaagat gtagttcttt
    30481 aaagacatgc attgctcttt ctattagaaa ctgaccagag ctggtaccgc caatgctgac
    30541 tagaattgtt ttcctattaa aagaaaactt ctttcgtaat tcctccctgg aatgattagt
    30601 ctttcttact atagagccaa ctctcatgat atttccttca cttggaccgt tttctgggag
    30661 aatgaccaga tcacattttt gtatcatgtt catcatagaa tgattcattt tcttttcaat
    30721 catggagcca attcctttca caaagctggt ttttagaaca tccgtaatca atactgtttt
    30781 cattttcttt tcttgagcta tgcctaacga tgcaaaatct tcatcactta caaccaatct
    30841 tgggttttca tcttcaatta tttttgatga gatagttttg cattctttat aataggaata
    30901 gtatctgaaa agccacctca gagggttttt cagattgcca ttcttaattt caaaaaatgg
    30961 tggtctgtat gcgttttctg cttggaatcc acaatctgaa aacaacgcgt aggccccctc
    31021 accgctcaca aattttattc tggtgtgatc aaggcattga gctatagcca catcacgagt
    31081 tgcgtgtcca agacctattg gactagaaaa gaaagctaca tctaccacac gttaaattgc
    31141 tgatgtgata atttctacat ttttcttttt ttacaaaatt ctcaaagttt aaatgcattt
    31201 ttacttggag cttttattgg gtccatagtc cagccggtta tgacgtcgcc cttacacggc
    31261 gaagatccag ggttcgaatc cctgtggacc cattaaaatc ctctaaatcc ttgtggtctg
    31321 caaagttcag gatgttcttt cagatatgat attttctcaa gtatcatgtt tttgtcctct
    31381 ggaaaagtgc cacctattgg atatgcgtta gagccatggt ttgctctaaa tatgatcgga
    31441 gattttgttt caatcttgct taccatattt tccagctcta ctagtgcatc agcatcatca
    31501 atgggaataa acggctcctt gaattttgtc ataaattctt ctcttatccc atcttctatg
    31561 tgtagtgtca gtgcaccaac ataatgcggt gatgttgcgc taattacttt ggcggtatca
    31621 tcaatgtgtt cttttgagtg tgtttttcca ccaagaccca gtattatcat acaggaaatt
    31681 acataccctg aatcttttgc tttttgacat gctttgatta tggtctgacc agtggctccc
    31741 tttgttactt ttttcaaaat tttatcagaa ccactttcta ttcctatgta aaacatggta
    31801 agaccagcat catacatttt tttcaaatct tctgctgact tttcaagcaa attttttggc
    31861 attgcatagc atgatacacg ttcaattttt gaaaatttct ggcgaatgta atcaagaatc
    31921 tgtatcattc tttctgtagg aagatttaac gcatctccgt ctgccagaaa aattctctgc
    31981 gtatcaggca tgtgttttgc tgcaagatca atttccattt ttatctcatc ccatggtctt
    32041 tccacatatt ctttggttct gtacatgtta caaaacgaac acacgttaaa agagcaacca
    32101 agagttactt gaaaaatgag agagtttgcc tcagaaggtg gcctgtaaag tggataatca
    32161 tatgaaagca taaccaattc ttgtattctg ttcaatttat cggttattct gggttaaact
    32221 cgtatttcat aggtattgct ttaaagcaag atttcaaaat taactagtgt tgaatacaga
    32281 attgccaaga gacttggttc caaagttttt tgacaaaact gcaagaactt atgataaaat
    32341 agtaaaccta acaacttttg gcagagataa atattggaaa aaagaaatac taaaaaaaat
    32401 tcaaaactgt aactccgtgc ttgatcttgc atgtggtaca ggaatactaa catttcaaat
    32461 agccaaacaa ctccctaatg caaaaataac aggaattgat gtcacagaaa gctatttgga
    32521 tattgctcga acaaaattaa aaccacatca caatatttcc tttttacatc aagatgcaga
    32581 aaagcttgat ttgaacacaa aatttgactg tattacttcc tcatacattc caaaatattg
    32641 taaatctgaa actctcatta aagtatgcat cgagcaccta aatccgggtg gaaaaatcat
    32701 tctacatgat ttcacatatc cacaaaacaa actagtcagg acattgtggg atttctattt
    32761 tataatattg aatctaatgg gattttttat tccaaattgg aagaaagtat tttcagatct
    32821 gccaaatttg ataagaacga caagatggtt ggatgaatac gagaatgcga tgagggcaca
    32881 caatttatca acagtgcgtc aatctctaac ctgggaatcg tcagctattc ttacaggtac
    32941 taagatggtc taatctgatc tcttcgtata tctgaaacca tatgcgttcg tcatgtacaa
    33001 tttcataatc acgtgttatt tttttcgtat cgggtgcaat ttcaatattg cccatttttc
    33061 gttgtacttt gattttgtat ttataaagta tttttcctat gccctctttt tttaacaata
    33121 actcagacat tatttgcttt ggaaggattt ttgaatcaaa ctttacagta gctagaataa
    33181 ttggaaattg ttcagaagat attactattt ttctaatgta ttctgtttct gatattggat
    33241 gctgttctat tactctaaac tgtactggtt tttttagaat ttctgaaact gtttgttcta
    33301 catgcccaac aggtgtatta agcaatgctt ttgacaaaag atctagaaca actgccacaa
    33361 gtatgatatc atatcaattc aattaagtct gtctcagaat gaaatatgat actgtaacta
    33421 caatgactcc aatactgatt aatttcaaca cagtttcctt tctgcgtgtt tctgaataac
    33481 tagactcttg gtgtttaagc tctttgtatt ttcttgaggt tttaatcaat atgtagctca
    33541 atatcccagc tattcctgct accacaagcg tttcggatga tatggtattt gcttgaattg
    33601 cagaacctgc aaaaacaggc agaactcccc atgatattac aaatgaaata ttgttatgaa
    33661 atcttccctt gaaccattca aggttataag caaaaagaaa gaacgactcg gcaattccta
    33721 ttggaaacaa taatggtgaa tccaaaaatg catagtataa tccaattaca aatgcgattg
    33781 ctaaagaaag taatgaaatt agcaggacat ttctttttga tagcgtaccc catggcttgt
    33841 ttttgctgcc tagtgaatcc agacaatgtg cagatattcc aagtgcaaaa aaatacaaaa
    33901 tacagattgc cgcaagtctt tctacagaaa aaccagctac aaatgaaccc catgcagcaa
    33961 atgatactac cattcctgta tatggcaaga acaatattcc tatgcctact ctgaaatttt
    34021 tagaaccaaa tttaggaaca aaccattctg ataacctatc ttttgacatt atttcttaat
    34081 gacttttgta acttatattg ttgattaaga tggaatttct ggaagtataa aatcaaatcc
    34141 agaaatcatt aaacttccaa caagaagact agtagctgca ctagctatgc ttgcatattt
    34201 gtatatcttg taagatttta gagactggat aaatcccgta gatatcatta tgccaaaaac
    34261 aagatttgat actattatac cagctacaaa cacgacaagc gcagtgacac ctagaatcat
    34321 tcctaacccc agtgacatgg caaaaacagc aagctgtgta ggggattctg caccaatccc
    34381 atgtatcata ccgattagaa acgatgagtg ttgaccatag ccattctcaa acactcttcg
    34441 ttgaggtata gtacgtccaa ataacttgct tcctaattta gaataaatga acagtatgcc
    34501 atttgcaatt attgtaaacc gtgttgcagc tcgaaattct ttttgtcctt tctttgcaag
    34561 atgataaaca atgtaacttc ctaaaatgat tagagtgacc cctactgctt ttcctattac
    34621 ttcatctaat atctgtggaa ccgaaatgcc aagtaatatt atggatgtac caatagaaat
    34681 cactacgagt gcatgtccaa gagaataaaa caatgtaaga aatgctgctt gtttttttct
    34741 tgcttgggtg tttgtaacat ctgttattgc taccatgtgg tcatagtcaa aaccatggcg
    34801 aaatcccaga aataaagatg tgagaaaaag aagtgagtcg atcacagact gcactgattt
    34861 tcgtattatt taaattcttt agagaattaa aaactgcttt tagtatttga taacgatgaa
    34921 atccttcctt tgtatttttc tttgtacaac gtcctgcaag aagtacaaca aaagaatctt
    34981 tcaaagtttg caaattttag aatacttggt tttccttcta cttgcccttt acaataatca
    35041 caggacattt tcacgagcat ttcctttctg agttttacat tgtggttttt tatcgtctta
    35101 tcatgtattt ggccaaggcg tgtgcctgtc ttggtttcaa gttttcccaa gtctaagact
    35161 ggcagaactg cttttatcag gcccacattg atcagcctct cgtatctggc ctttactgta
    35221 ggtgtgctta ccttgatctc tcttgaaatc tgtctaaatg atttcctacc atcttttaca
    35281 agtgattcca atatcgaaat gtcaacatca tctaacttga agggcatatc tacttgtaga
    35341 ttctttccat atttgtacat tttacaaatg ttaagattgt gttgttttac atttgtaaag
    35401 tccgacattt aattatccat ctgcctctaa aataaacatg tcaatacagg ttacagctag
    35461 aaaactaaca acaatagttt ctttcattct cttctcactt gtttttcttg gcataatttt
    35521 ttcactgact aatgatgtta tggttaaagg aaatgaagac gtatcgtatt tgtcatggtt
    35581 agtaatttca tatgttgcag gtctttctat gataattttg ccatgcactc taccgcttgt
    35641 cttcataata gttccattaa gcatgggaca aaattctaag aaaggattag tcatggcttt
    35701 actgtttggt attggcctaa caattactat aacagcttat gggactggtg ttgcaatact
    35761 tggcaaaact gcatcattag atcaaatctc agttgtgatg tttcttattg caggcattgc
    35821 ggcttttatg tttggattat cacagctaaa acttgttaca ttaaaattgc catcttattc
    35881 agggactcca aaattcatac aaaaccgtgg agattattcc aagtctttct ttatgggaat
    35941 tttattggga aatgcaggag ttggttgtcc aaatccattg ttctattggt tgttaattca
    36001 tattgcaaac gttggaagca tagaagttgg tgcatcactc ggacttgttc atggagtagg
    36061 acgagcaatt cctctcatcc tcatgtctgt tcttgcaatt atgggcatta atgccacaaa
    36121 gaatcttaca tcaaaaagag ttcctataga aaaggcgact ggctggatgc ttgttgtaat
    36181 tggttcattt ctaataatca acggattacc cagtggacat caatggtacg aagaaacatt
    36241 cgtgcacata gcatggaata acttggtgtc tatcacacca attccgccag agttccatgt
    36301 tggccaacac atgcatgaaa atgtgtatgg tattgaaatt tctaaaaact acatcccaac
    36361 tctacttttt ggtttgataa tgacaccagt tttatggtac ctgataaaga agagaaaagt
    36421 ggtgatatca tgaaagatcc tgtttgtgga atggacgtgg gtgaaaaagg cgagtcgctt
    36481 actcatgatg gaaaagaata tcgattttgc tgtgctagct gtagatgggc ctttgaaaaa
    36541 aatccagaac aatttgtagg aaaggaatca tgaaagtaga aatactgaca accccaggct
    36601 gctcaaattg cagtgtcgtt gaaaaaatgt ttgatgaagc aggcattcca tacaaagtaa
    36661 tagacgtgac tgaaaatcca gaatatctac aaaaatatcc aatatacaca gcgccaggtg
    36721 tagtcataga tggaaaatta gaatttagtg gagtgccaag aaaaaaagaa ttcaatgaaa
    36781 aattggaaaa actaagatca taaggtatca agatattaga tttgatttat ttgtaagtat
    36841 catacagtag ttttgtgaaa caagagtaca aagaatatct aaagctaaac aagaacatca
    36901 tccttggatt tgcagcatct atctgtgttt caactgtagc agcacatctc ttttcagaac
    36961 aagaagctta ccttaacgcc acattcacac tcgtagtaga ctatgtagtg tatttcacta
    37021 catttggggg cttgtattat attgataaca ggaagaaata cagactagaa tctggagaaa
    37081 ctgacaagat ttcactaaga aaggatctca taaagataat ctcttctctg ggaattggag
    37141 aagttgtata tacaatagca cgatggtttt tacaatacta ttttcttaca ataggatatg
    37201 acgcatatct tgcatctttg gtatcgcaat ctacatccac tgttatctat atgcttgtag
    37261 taaacatcag tgtcaaattg acaaggttgt acaaagatgg tacttagtat ctacgttgat
    37321 ggttctggag gttcagaatc aggatttggt ttttttgtca aggaaaccgg ggagtcattt
    37381 tataaaaaag aatcaaacat aacaaataat caggcagaat acatggcaat tcttgcggta
    37441 ctaaaaaaat ttacgaatta tacagatgaa atcaccatat atagcgattc caaaaacact
    37501 gtttctcaac taaatcacga atatgcaata aatagtccag aacttagaac gcttgccatg
    37561 gaagcttgga acctgatagg caagtattca aatttaaaaa taaaatggat cccacgaaaa
    37621 gaaaatttag ctggaaagat gcttgggagc taagtcttac ataaacgcaa agaatttgca
    37681 ctaatcaaac ggatagactt attatacaaa tcaatttgat ctcattttat taaaatggca
    37741 gaatctgttt ttctttctcc aaaatctatt gcaataatcg gtgcatcaga caaggaaggc
    37801 agtgtaggac gtgcaattac atcaaacata atgaagggat acactggaaa aatattccct
    37861 atcagtccaa ctagagatac agtatttgat aaaaaggcgt acaaaagcgt cttagaagtt
    37921 ccagaagaga ttgatcttgc ggtagttata acaaaaaatg acatcgtgcc ctctgttctt
    37981 gaggaatgtg gaaagaaaaa actcaaggga gtcattgtaa ttacagctgg attcaaagaa
    38041 gtaaatgagc agggagctgc acttgaacga aagcttggag aaattgcaaa acaatacaac
    38101 ctgagaataa ttggcccaaa ttgccttggt gtgatgaacc ttgcaccaga aacaatgatg
    38161 aactcaacat ttctcaaagt tactccaaaa tcaggtggca ttgcacttgt ttcgcaaagt
    38221 ggagccattt gtgctgcact agttgaggat gcaagcgctc agggaatcgg attttctgca
    38281 gtaataagta tgggaaataa agtcgacatg aacgaagtcg atatactaca gatgcttgca
    38341 gaacatggtc aaaccaaagt cattgtgatg tatcttgagg atctaggaaa aggcccagaa
    38401 tttcttaaaa tctgtaaaca aatcacgcgt cttaatccaa taaaaaaacc aatacttgtt
    38461 ttaaaatctg gcagaagccc agagggtgca aaagctgcca tgtcacacac tggagcactg
    38521 atgggctctg acgaaatcta tgatgcattg ctaaaacaat caggcgcaat cagagtagat
    38581 acaatggaag agctattcga ttatgcaaca gcattctcta aacaaccact gccaacaaaa
    38641 ggagatctgg tcatagtttc caacgccgga ggacctgcca taatatcaac tgatgcctgt
    38701 tccaagcttg gaataaagat ggcaaagatt gaggaaatca gaccaaaaat aaacgcagta
    38761 attccaccgt ggggttcatc aagaaaccct gttgatatag ttggagatgc agatttcaat
    38821 agattcaaca atgtgttaaa tgaagttctt gcacatccaa acgttggttc agttattgca
    38881 atgtgtactc cgtctgcaac cttggattac aataaacttg cagaagtaat agtcgatgta
    38941 tcaaaaaaat acaacaagac aatgtttgca agtttaatgg gtctggatga aggcataaaa
    39001 aataaagaaa tacttgcaca aggaggcatc ccacactaca actacgcaga gactgcaata
    39061 cgttctctta aagcaatgct gcggtttaca gagtggtctc aagccacaga aggtacagtt
    39121 cgtaaatttg ctgtaaataa gaagaaagtt gaaaaagtgt ttgcccaagt caaatcggaa
    39181 ggaaggaaaa atcttcttga agaagaaggg caagaggtct tgcgagcata cggatttcct
    39241 gttccaaaaa gcatacttgc agcaaaaaac agtgaagctg taagcgctgc aaaaaagatt
    39301 ggttttccag tagtaatgaa gattgcatct cctcaaatca tccacaagtc tgatgcgggt
    39361 ggagtaaagg ttggactaaa aaatccaaaa gaagtaaaaa ctgcatttaa ggaaataata
    39421 aaaaatgcaa aaagatacga caaaaaggca atcatcaaag gcgttttagt acaagagatg
    39481 gttaagggtg gaaaagaaac cataatcggt tcaaaacaag aaccaggatt tggtcctgtt
    39541 gtgatgtttg gtatgggagg aatttatgtt gaagttctaa aagatgtaac attcagactt
    39601 gcgccagtta ctgataaaga agcagacgaa atgatttctt ctattaaaac aaacaagttg
    39661 ttgcaaggag tacgtggcga aaaaccatct gacataaaca agttatctga atgcatacaa
    39721 cgactttcgc aactggtaac tgattttaca gaaataaaag aacttgacat gaatccagta
    39781 ttggtttttg aaaagggcaa aggctgtaaa gctgttgatg taagaatcgg tcttacctga
    39841 tcatagtgta attttgatgc caaataggat gcctaagatt ttatattgct ttgaagaagt
    39901 aaaagatgca tgccaattag gactggtgct gaatacatag aaagcctaag aggaagaaaa
    39961 ctgaaagtct atctttttgg tgaacgtgtt aaagaaccag tagatcaccc aattataaga
    40021 ccctccatta acgcagttgc agagacttat gatttagctg tacgagaaga agaacttgct
    40081 actgtcaaat cttctctaag tggacaaaag attaacagat ttttgcatat tgcagaaagt
    40141 gctgaagatc tagtgaatca aaacaaaatg caaaggaaat tgggacaaaa tactggaacc
    40201 tgtttccaaa gatgtgttgg tatggacgcg ttaaactcac ttcattctgt tacttttgag
    40261 attgacgaga aacacaagac tagttatcat aaaagactga ttgattttat aaaaatggta
    40321 caaactgaaa atctcgtaat tggtggagca atgactgatc caaaagggga tcgaagtaaa
    40381 gctcctcatg aacaagaaga tccagacatg tttgttcgag tggtagaaaa aaatgacaag
    40441 ggaattgtaa tcaaaggagc aaaggcacac cagactggtt gtataaactc acactggatt
    40501 atagtgatgc caacaatgcg attgcaagaa aatgacactg attatgcagt tgtgggtgct
    40561 gtaagagctg atgatcctgg aatcacatac atctatggca gacagtcatg tgacacaaga
    40621 agtatggaag aaggtgatct agattctggt aattcgaaat tctcaggaca ggaagctatg
    40681 ataatttttg atgatgtttt cattccatgg gaacgagtct tcatgaatgg cgaatatgaa
    40741 tttgcgtcta tgctcgttga gagatttact tgctatcaca gaagaagcta tgtatgtaaa
    40801 acaggattag gtgatgtatt gattggtgct gctgcttcaa tagcagacta taacggcata
    40861 ccaggcgtat ctcacatcaa agataaacta gtagaaatga ctcatctaaa tgagagcata
    40921 tatgcagcag gaatagcctc ttcatatcaa gcatacaaaa caaaatctgg cgtatggata
    40981 aatgatgaca tgcttgcaaa tgtgtgtaaa cacaatgtta caagatttcc atatgagatt
    41041 ggtagattgg ctcaagatat tgcaggggga ttattagtta cgcttccatc cgaaaaagat
    41101 tttagaaatc cagaaactgg cccaatcctc aaaaaatacc taaaaggacg caaaggtgta
    41161 gatgttgaaa atagaatgag aattttgaga ttgattgaaa acatgacaat gggtagaaat
    41221 gcagtaggat atcttacaga atcaatgcat ggtgctggtt ctcctcaagc tcagagaata
    41281 aacattgcac gcctaatgca gcttggttac aaaaagaagc tggccaaaaa tctggcaaat
    41341 gtaaaagaag aacctgatct tactcctgaa caagctgaat attttgagcg agtattcaaa
    41401 ataagtaaaa gcgttgctgg tgtaaagaac aactaatttt atttttgttc tgattctttt
    41461 gcttctgaaa tttccttaat gctatcagtt tttgtatatt cattgtcttg tacatgttca
    41521 atctcggccg atgttatacc cggacttggt gtgttatagt catctgtagg aggctggtcc
    41581 ttgggtgatt catctgatgg gttgtctgaa cggcctttgt gttttccata ttgtttgagg
    41641 atcatgattc cgacaatgac cgcaattatg atgtagttta tggggggagt tagaattctt
    41701 gtaacatatc ctatctgtgg gactacatac gcaaccttgc ctatgtattc ctcttctgtt
    41761 attggaaagt ctgttcctgg tattgaagct ggattggcgt caccttttgt tctgatgaca
    41821 cgaggatctt catcaagaat ctcagctact ctgtgtacta ttactctgtc gtggccagac
    41881 ggtctgtcaa aaactataat gtcacctttt ttgagctcct tgaaaggatc atttccctgt
    41941 acaacaagaa catcaaacac attgagattt ggttccatac ttccgcttga aacaacgtaa
    42001 aatgggttat cggttccaaa gacaattcgt aatccaaacc aaatgatcgc aacaccgata
    42061 acaactatta tgatgtcttt tgtaattgaa gacattccag aatgtttggt caaattatac
    42121 tttatcctgt aaatgttgga aaataaatct tgacatcttt cattctactt taatttttta
    42181 ccgcaacttt cacagaattt tgcatctggc ttgttttcaa aaccgcatcc gttacatttt
    42241 gagcttgtat cagaaacctg atttcctaag agaactattt ctccaatttt ccttatttct
    42301 tcccacttta ccatgatatc actgccgtca ttttttgtaa caaccaaaac tattccagac
    42361 tgaccagact gaagcccgat ttgttttact gtgccaacct ttcgtgcgtc tgaatcataa
    42421 actgctatac ctgttataga attaaaagtt gtaacctccg gtggcgcttc ttgctgtgaa
    42481 tacgtatgtt gtggttgcat gtaatctgat gcaggcgaag attcaactag aatctgtgtt
    42541 gcaagtgatt ggggtttggc tagccctacg tcggcagact cgtttggttt actaaaagtt
    42601 ctaaattctg caagaacaga accgcagttt gtacagacgt attgtccctt ttctacaagc
    42661 attaggtttt ctgctacaag ttcgtttggt gtttcagatt ctatcttagg actgccctca
    42721 aaatcttttt cacaagtatt gcaaaaatat ttagaaattc tattaacatc aattcttcca
    42781 acaggagcca gaaatagatc aggtccgcct aagttccctt ttctttgctg ttcgtcagta
    42841 actgatgcca tgatgtatcc accagaacct ctgagctttt tcagtcgcag ctctgaactc
    42901 ataatttagg tgtataaaaa aagtcattta agtatgtatt cagttgctaa atattgcaaa
    42961 aaatctcttt tagataatct atctgatgac taaacattat ataatttgta ataaccagat
    43021 gatttttttg aatattttgg aaagggttat tagaggtaaa aactgagtta aaacatcgaa
    43081 aataatgacg gaataagcaa tgagctttct ctgtcattgc ttaagaaaga tgaaagaaat
    43141 tgacaaaatt tgaagaatta gggattaaaa aatccatatt ggacggatta aaggaaatta
    43201 acttccaaga cacatttcca atacaagctg ctgcaatacc agttatactt tcagggcaag
    43261 atgttatcgg gcaagcccat actggaacag gaaaaactgc agcgttttca ttaccaatac
    43321 tgcaaaatat tgttccaaag ggaggaatac aagcagtaat acttgcaccc acaagagaac
    43381 tcgcagtaca gatttcagga gagattagta aatttgccaa atatactgga ataagaaatg
    43441 taactatcta tggaggacaa agcattaaca atcaattccg agatcttgat agaggcgtag
    43501 aaattgttgt agcgactcca ggacgattaa ttgatcacct gaaacgtggt tccattgaat
    43561 taaacgatgt aaagtatgtt gtacttgatg aagcggatac catgcttgac atgggattca
    43621 ttgatgacat aaaattcatc ttagacttgg ctcctgatga aagatcaacc tcgctctttt
    43681 ctgcaacaat gccggctgag attctccgac ttgcggagaa atacatgaga aatccacaac
    43741 aaattttcat tgatgcagat gaccttagtg gtgaaggaat agaccaagcc ttcctagtaa
    43801 tacgtgatag ggaaaaaaca gattttctat ttaaattcat aaaagaaaat aagaacggtc
    43861 aaaccatcgt gttttgttca acaaaagaca ggacaagaag ggttgcccat gagcttggaa
    43921 aagaaaattt caacgtggta tcaattcatg gtgatctttc tcaatttaga agggataaag
    43981 ccatgtatct gttcaaaaaa ggtaaagtag acattcttgt tgcaactgat gtagctgcaa
    44041 gaggaattga tgtgcctgaa gttgcacttg tgataaacta tgatgttcca aatcaaaaca
    44101 tggtttattt ccatagaatc ggaaggactg ctagagctgg tgcaaaggga agggcaatca
    44161 cactcgtttc ttattcttct gttggtgact tccaagcaat tgcaagacaa accaaagtgc
    44221 aaatgacaga tctaaacaaa gaacttggaa taacagttaa aattccggat ccattaaaaa
    44281 gagatccagg tcataggggc ggtggtcatg gatatggaag aagatatggc gatgatcgca
    44341 gatatggtgg agaaagaaga tatggtaaca gacaaagtca tggcgatcga ggatcaagtt
    44401 ataataaaaa atactacggc ctaagaagtc gctggtaaaa ttagcacttt tgcactgtca
    44461 acatatttag ggatgtaata attatttatt tttgataaaa atgggaaacg ctaaagcagt
    44521 cgacacaaaa acttggcaga ctgaagtaat gcagtctcaa ctaccagtct ttgtagactt
    44581 ttgggcagaa tggtgtggtc catgtaggat ggttggacca gtagtggaag agcttgctgg
    44641 cgaatactct ggcaaggtaa gctttgtcaa agtaaacgta gatgagagcg gtgaacttgc
    44701 atcaaaatac aacatattca gtattcctac acttgccata ttcaataatg gtgaaattat
    44761 agcccaacag gtgggtgccg cttctaagga ctcgtacaaa aacatgatag aaaacgcctt
    44821 aaaaaaaatc tagacctaga acaactaggc ctttaatttt ttcaaatcta aaataattaa
    44881 taacggacca aggaactttt tgtatgtctt ttggagaggt agatacactc aatcttctgt
    44941 ttgataaatt acaaaacctg ttaaacgatt ctcagggata ctatgaatca tttcttgatg
    45001 ccaacacact ttacaaacaa ggaaaactat cagaaaaaga gttttttgaa aagctcgggg
    45061 attatgttgt tgcgtattct gcattagaat ttcttgccgt caaagtcata ttcgaattaa
    45121 aaaaatcaat tgataaaata gctagtggtt ctacaggtac aacatcaggt tctacaactg
    45181 ttggaatggg aggatttgga atgactccac cagctggtca tatgatggct caaaatgccc
    45241 caatgcctgg tcgccctcca tctattatct ccgcttctgc atttaatgca cctggtgctt
    45301 taccaactcc agatccatct ctattgccgc ctagaactaa tctgccaagc tgtacatctt
    45361 gtggttctgc aattagacca aattcaaaat tctgtactaa atgtggaaat aaaatacaat
    45421 aacataaata atttttaata catttcacac aaaacgaaat ctgattatta tgtcaatctt
    45481 gtgttgcacc acactctgtg cagaattttg cattagatga aatcttgatg ccgcattctg
    45541 aacagaattt agtatcagaa cctgattctg tcttaagact accatatatt cctccgctac
    45601 ttgatttaac agcgccagtt ggaacgtatt catcatcaat tatttgtact ggtgcaaaat
    45661 ctttctcctc cacatacgcc atcattctgg gcattgtttg accaggattc tttgggtctg
    45721 gaattatgtc tccaagccaa aatgcaaatc ttgtcagcgt aagatcaggt gttgaaaaag
    45781 tcaacgtatg attagacata tagtcttctg cagctgcttt tccattaaac actttcatga
    45841 atgcactatt tctgatagct gtatattgtt ttttggtata aagtggaccg agggggattt
    45901 gaacccccga catccgcgtt gcgaacgcgg catcactacc actagactac cggccctttt
    45961 gttaccaaga atctcctccg tttttattca tttactctca aagaataata ctgggattca
    46021 aagaaaaaac attggatgac ttttgatacc gtaattactg attcacacgt agttttacct
    46081 agcggaattg ttgaaaaaaa tattgtgatt gataatggta aaattgtcaa tcttacaaac
    46141 gacattcctc aatgtgatgt taaaattaac ggtcgtggtc tagtttcaat gcctggtgct
    46201 atagaccctc atgtgcatta tggcgtgtat tcaccaatag aaaaagccgc gattacagaa
    46261 tcacgtgttg cagcaattgg tggagttaca acaatgatga ggatgttgcg attaggcggt
    46321 tcgtacaaaa aacttcttcc atcgcatctt tcccaaagtg ctactactca ctatgtagat
    46381 tatgcaatac atgcgtcaat cctacagaaa gatcaaatag atgaaatgga attttgcaca
    46441 aaaaacggaa tccaatcatt caaaatttac atgaatcttg gagaagatat aggacatgta
    46501 tacatggaca tggagcctga aacaaatcaa ctccatgaag aaaaagttga gatgaattta
    46561 gacatgattg aaaaaataat tgcgaatgct gcatctcttg gttgtcctgt tttagttcat
    46621 gccgaagatt atcaaatgtg ttcttgcgga atccgtgaag caagagaaaa aaataaggat
    46681 ggactgggag catggtcaga gagcagatct gtggaatctg aagtcaaatc catacgaacc
    46741 atctctaaaa ttgcaagaaa aattgggtgt acactgtatt ttgtacacat tggttcagaa
    46801 ggcgcaatga atgcaataaa agatgaaaaa aagaatggca caaaaatcca tgttgaaact
    46861 tgtcctcatt atctcactct ctcatttgaa tcccagaatg gatacttggc taaagtaatg
    46921 ccaccaataa ggacttcaaa agatgtagat gcagtatgga aagagatcca caatggtatg
    46981 gttgacacaa taggaacaga tcacgttgca aatcaactaa aactaaagct tggaggaaat
    47041 gatgtgtggg gcgcactagc tggatttcca ggtataggta ccatgttgcc aatattgata
    47101 agcgagggtg taaacaaagg taggatcaca ctacaacaac tgatggaatt tacaagcatg
    47161 aatgcttcta agatttttgg aatgtttcca aaaaaaggta tcctgcaaaa agactctgat
    47221 gctgacatcg ttatgataga catgaaaaaa gagaataaag tgtcaagcca acttcttgat
    47281 ggattttcag actatacagt atatgatggc tggaatctaa aaggctggcc tgttaaaaca
    47341 attgtgaggg gtgaaattgt ggcagaagat tttgaaataa ttggcaaaca gggtcatgga
    47401 aaatatgtca caagatcaaa cctacgttag aatgtcatat ttgccattct cttttacttt
    47461 gtaaatgata tcgggttttc ttgtaaatat tccgacttct acgactcctg gaatttgtat
    47521 gattttttca gccaattctc ttggttttga tatctcgcca aaatcgcaat ccaaaattat
    47581 gttaccgttc tctgtgacaa aaggataacc acgttctatt gtacgtagag ttggctttcc
    47641 agaatatttc tgtatgtgtc ttgctgcagt gtttctagca aatggatgaa cttctaccgg
    47701 tacactgtga tttagttttt ttacaaattt tgactcgtct gctatgatta caactttttt
    47761 tgctatgttt atgagtatat tctcacgtaa aagtgcacca ccaccacctt ttatcatata
    47821 tttttcttca tctatctgat ctgcaccgtc gaaaaccaga tcaatgtgtt caatctgatc
    47881 tgcttcaatt attggaatgc cgcctttctc tgcttctaat ttgatttgta gtgatgttgg
    47941 aactgctttt actctaatgt ttttttcttt caaatatgat gaaagtgatt tcacaagtgc
    48001 tgtagcagcc cttccacttc caagtcctaa aacataattg tcttgtatca gtcttaatgt
    48061 gtccgctgac aatgccttta tggcatcatc tattgtcaac tattctacct ctacttgttc
    48121 cagtttagag agggctttca ttatttcgtt ttttatttta tcaatgtctt tgtcatcgtc
    48181 ttctaatttt tcatcttccg gtattttgac cgccgctgga atctggatct ttttctcaac
    48241 aggaatctgt gcaggttctg gttttggagt ttctatgact ggttgaatct caattttggg
    48301 tgtttctact actgactgaa tattcatttt gggtttttct ggttctgcag ttctacttgt
    48361 catgatttct tctattattt gcttttgtgg tgtaacaact actggtttga caagctcaac
    48421 aggaaattct gacatcctat ttggtatttc tgtaagtgtg atgagctcaa ctgatttatg
    48481 aggtgccgca atggcaactt caattggtgg caggggagtg tagggttctt taggtttttg
    48541 aatctcattt ttaatttcta ttttcttttg tattggtttc tcaggaatac tctcttttgg
    48601 aggttcaagt tgttttattt gaggcggtgt cattgtagcc actgttatct tggaagaaat
    48661 ttcatggagt ctctgatcca gttgtgaaag tttttgatcc attaatgtga tcaatccatc
    48721 tcctagaggg ccaagatctg ggtgtttgct tgccgcttct agtttttcaa tttttgctat
    48781 tacaa
//
LOCUS       DATIDL010000157        11796 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrosopumilaceae archaeon isolate SMAG_U16342
            k141_2214929, whole genome shotgun sequence.
ACCESSION   DATIDL010000157 DATIDL010000000
VERSION     DATIDL010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935836
            Sequence Read Archive: SRR25158326
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosopumilaceae archaeon (soil metagenome)
  ORGANISM  Nitrosopumilaceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrosopumilales;
            Nitrosopumilaceae.
REFERENCE   1  (bases 1 to 11796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11796)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/04/2024 05:09:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,520
            CDSs (total)                 :: 1,474
            Genes (coding)               :: 1,474
            CDSs (with protein)          :: 1,474
            Genes (RNA)                  :: 46
            rRNAs                        :: 1 (5S)
            complete rRNAs               :: 1 (5S)
            tRNAs                        :: 43
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11796
                     /organism="Nitrosopumilaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2214929"
                     /isolate="SMAG_U16342"
                     /isolation_source="soil"
                     /db_xref="taxon:2202732"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="VLB45_07210"
     CDS             complement(<1..194)
                     /locus_tag="VLB45_07210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05517.1"
                     /translation="MKIILFLILILFSGLLPAYGDPLLKDQDLVIEKFVSGLSLPTTM
                     SFVGDDLLVLEKNTGKVRLV"
     gene            complement(252..431)
                     /locus_tag="VLB45_07215"
     CDS             complement(252..431)
                     /locus_tag="VLB45_07215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05518.1"
                     /translation="MPGTGEICQQSGTYRFVMHVDGSIGCHPRSEKLVIQVSKGEIFP
                     PVKSCKKTAIWILVE"
     gene            645..944
                     /locus_tag="VLB45_07220"
     CDS             645..944
                     /locus_tag="VLB45_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007549924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HSD05519.1"
                     /translation="MTTINQLRSGMTRLDPIEGTIESLGEPRTVNLRTGGQAQVADAV
                     LKDDTGQIKLTLWDAQIKMVKAGSKVKVENGYINSFKGENSLNVGKYGKLNIIEF"
     gene            complement(951..1727)
                     /locus_tag="VLB45_07225"
     CDS             complement(951..1727)
                     /locus_tag="VLB45_07225"
                     /inference="COORDINATES: protein motif:HMM:TIGR00162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome assembly chaperone family protein"
                     /protein_id="HSD05520.1"
                     /translation="MSSRSKITIEISEMPKLKNPHLVCGLPGSGYVGKLAVDYLVDEL
                     KGVQFANIFSSSFPPQVSVQVNGTVDLMKNTLYYCKNKTDDIILLTGDAQPVTPEGEY
                     EMAEEIIGICEKLGVKNIYTLAAYITGTFSKTPKVYGTSTSLDAVKEFSKQGISTMSG
                     GSITGMNGLVIGVGKTRGINGVCLLGETSGYVVDAKASKAVLEVLAKMLGLKLDMTTL
                     AKRAQDTEHLIKTIEEQMGQRPPQDQLPLPAQDKKLGYIS"
     gene            complement(1773..2657)
                     /locus_tag="VLB45_07230"
     CDS             complement(1773..2657)
                     /locus_tag="VLB45_07230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05521.1"
                     /translation="MTTLSKLAASYKNKKIEATKSRRQSEKKLHSTISLKRRSSSGLS
                     SLERRKEQALQKKESISQLLTQYMAQRDSVQRLKLAAEARLKQEEDAKEKTLQEGEFS
                     ESPEDKARASEKIRMIDAKIAELHSEIKQRNAAEERLVKLIADTSKSKSKLDSQMKQQ
                     SHAKPLLLEQLQSSTKAEAALRSQVQTRLKQEAQANRNLIKITERLEILKAKKRKAQR
                     KARKAKRKAKAKKAKLKTKRKARRVKTRIKKAKTKRKIKTKKTKSKAKRKSKAKSKTK
                     RKTKKVKRKAKRSKSRRK"
     gene            2846..3127
                     /locus_tag="VLB45_07235"
     CDS             2846..3127
                     /locus_tag="VLB45_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05522.1"
                     /translation="MKTFQVDNESQASEAAAEMVKIGFSSQKSAFKVLMTNEKDLAKK
                     IGFLVVNEINFGLRKIKQERDVMYWIYHHDKSHYAMVLISSKVLHELGF"
     gene            complement(3116..3769)
                     /locus_tag="VLB45_07240"
     CDS             complement(3116..3769)
                     /locus_tag="VLB45_07240"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HSD05523.1"
                     /translation="MQQWIFDIRSKKFLLLLVLFIAVTILVYGKITKPLDDAVITYAQ
                     SLAGNQIQDLTMEIFTEIGSVFYMVIFCVILFVKKSTRRLGLVLILSILAGTIVSAYV
                     KDFVGQERPKLDFQGTPFPIGLEQDSSFFGIGSFPSGHATRTAAFAFVMGFALSHRFP
                     RGCYIVWVYPLIVSISRVYVLQHFPMDVIGGVLLGIIIANIVSKKLKLHLIFEKSEA"
     gene            3906..4067
                     /locus_tag="VLB45_07245"
     CDS             3906..4067
                     /locus_tag="VLB45_07245"
                     /inference="COORDINATES: protein motif:HMM:NF018096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1059 domain-containing protein"
                     /protein_id="HSD05524.1"
                     /translation="MTYQVICKDYGNECDFKIRTESESELVQKVKKHMEDEHGIDLPK
                     EQIIQMSKL"
     gene            4132..5340
                     /gene="pgk"
                     /locus_tag="VLB45_07250"
     CDS             4132..5340
                     /gene="pgk"
                     /locus_tag="VLB45_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010193635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HSD05525.1"
                     /translation="MRLLTLEDFDTKGKTVFLRVDMNCPIDPKTMEILETSRIRESTE
                     SLKDLEGAKVVIASHQGRVGNKDYTGMEKHAKALEKILGRKIKYVEDVIGAEAQREIK
                     NMKNGEILLLDNLRLCAEENYEFSPAEAAKTIMVTRLAKLFDLCVLDSFPSSHRSHPS
                     IVGFSSILPACAGRLVEREVRKLDEIMTVAKGAHVVVLGGSKVADRLEAMKTLIQNGR
                     ADQILLTGVIANVFMRAQGRIKFPLGIKREEEIVSKAHSLIGEYPDVFSTPVDVATDR
                     GGVRVEMDVRELDKGDQILDLGPKTVEHYTKSIQSAGTVFMSGPAGFFENDNFKFGTE
                     SLLKAVANSFATTIVSGGHLTAALKRYGLTEKINHISTAGGALVLYLTGAKLPMIQTL
                     EEAAEKYRTK"
     gene            complement(5333..5671)
                     /locus_tag="VLB45_07255"
     CDS             complement(5333..5671)
                     /locus_tag="VLB45_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05526.1"
                     /translation="MTDNQASILLQVRKIIFEKFNDVNLRFTNDEVFEILKNQDGAKS
                     LTIDDMKPFFNQLHEEGLLRPIAQDFTTQWFKLFGLVEKSKCNSCNLEIYLGTLEEKR
                     CPNHACKAAI"
     gene            complement(5673..6113)
                     /locus_tag="VLB45_07260"
     CDS             complement(5673..6113)
                     /locus_tag="VLB45_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018039412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HSD05527.1"
                     /translation="MSSADIYREIILDYYRNPRNYGKIENPDIAKRDSNPLCGDELEM
                     HINLKDNKVSDVKFTGKGCAISQASASMLTELIMGKDFDYVKKLTKEDILENLGLPDL
                     GPARIKCALLSLKVLKYGIYSYVSEKLNDVASADKIKEEASGLF"
     gene            complement(6110..7354)
                     /locus_tag="VLB45_07265"
     CDS             complement(6110..7354)
                     /locus_tag="VLB45_07265"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HSD05528.1"
                     /translation="MQASTINIENIRKDFPILQRKVHKDKPLVYFDNAATTQKPIQVI
                     DSIHNYYLNYNSNIHRAVHQLAEEATAAYEMTRDKVTKFINAKNREEIIFVRGTTEAI
                     NLVAYSWGRQNVQKDDIIVTTEYEHHSNIVPWQLLTKEKGAKLEYIGVDDNGELILDH
                     LDAYLETGKVKLVTFSQMSNVLGTISPTEEIIKRCHAKGVKVLVDAAQSVPHMKVDLQ
                     KLDCDFFAFSAHKMLGPTGVGVLWVRKEILENMQPFNGGGDMIREVHKYETSWNDLPY
                     KFEAGTPNIADVIGFSAAIDYLENIGMDKVREHEIELTKYALDKISAIKGIILYGPSD
                     VKKRGGVISFNLGDIHPHDLATIIDEDGIAIRSGHHCAQVLMERLDVSATSRASFYIY
                     NTKEEVDVFIRSLNRARELFRI"
     gene            complement(7358..7678)
                     /locus_tag="VLB45_07270"
     CDS             complement(7358..7678)
                     /locus_tag="VLB45_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007549914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme iron oxygenase ferredoxin subunit"
                     /protein_id="HSD05529.1"
                     /translation="MSILSEWVNVCSTKKLGKGELYSFDHNDKKIMLANMNGKIYATD
                     RICTHAEADLSAGILGEEGVTCPLHLSTFNLETGMPQNLPAEKPLKTYNVKIEQNEIY
                     VEVK"
     gene            complement(7675..9075)
                     /gene="sufD"
                     /locus_tag="VLB45_07275"
     CDS             complement(7675..9075)
                     /gene="sufD"
                     /locus_tag="VLB45_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HSD05530.1"
                     /translation="MTSFLLSALGQNHVDEISSANNEPAWLKEYRENSFSIYKELPYE
                     VSPLYNKYSDANKMDPEKISFSLSSDSTVPDFVKERMAEIANGASIVQIGTNIHQINL
                     PSELKSKGLIICSIHDAIKNHSDIVKKSLDLTDPKKDKYIALNNAFFNSGFFIYVPKN
                     LILDKPIHAISSLSVDGTSTMSRNIVVAEENSKAMIVQELYAPKNTKQQAYLELLDVS
                     VRQNSELDMVTLQAMDQNAINFSTRTGKIARDARMNWYLGLFGALLSRYKIDNYLNEI
                     GATANDTEVVFGNKNQSFDITSNLIHVAPSTTGRVLEKSVLKDTAKSLFKGMIRIEEH
                     AHHSESYLAGHSILLDKGAKSDSIPGLEIFTNDVKATHAASVAQLDDEQLFYLGSRCL
                     DKSEAQKIIVEGFLEPLSRKMSYQIRAWINYLIDCKWLGKDLTIKTDEQLKAILEVEE
                     TRYRETDIFESHYKYR"
     gene            complement(9106..10506)
                     /gene="sufB"
                     /locus_tag="VLB45_07280"
     CDS             complement(9106..10506)
                     /gene="sufB"
                     /locus_tag="VLB45_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HSD05531.1"
                     /translation="MATEDLNMDYSKYDFKDSTEMYVYLSKKGLSRGTVEEISKLKGE
                     PQWMLDFRLRSYDIFMKKPMPNWGGDLSKIDFNNIYYYAKASEKTEKSWDNVPEDVKN
                     TFDKLGIPEAEKKFLAGVGAQYESEVVYHNLREDLAKQGVKFLDTDTALKEEPEIFRK
                     YFGKIIPPEDNKFAALNSAVWSGGSFIYIPPGVKVDLPLQAYFRINAENIGQFERTLI
                     IADEGSEVHYIEGCTAPVYSSESLHSAVVELIALKGAKLRYTTIQNWSNDVYNLVTKR
                     AYAYEGARVEWIDGNIGSKLTMKYPGIYLMGPRAHGETLSVAFAGKNQHQDTGAKMVH
                     LAPNTTSRITSKSVSRANGRTTYRGLLHVAKGATNVKASVRCDALLLDDTSKTDTYPY
                     MEINQEDATVTHEATVGKIGDEQIFYLMSRGFSEEDALTLIVNGFIEPFTKELPMEYA
                     VELNRLVKLEMDGSVG"
     gene            complement(10594..11388)
                     /locus_tag="VLB45_07285"
     CDS             complement(10594..11388)
                     /locus_tag="VLB45_07285"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HSD05532.1"
                     /translation="MITGKTIAITRSKEDSEEFVQLITKASARAIPLPTIELVSKGDK
                     IVDEFLDAIKQDDPDFSVFMSSKAVKLLFDTARKVSKYEKLQLAVANTNVIAVGPKTK
                     TALEAEGIRVAHMPQRYSSLGVGEVFTRLNAEGKKAIIPRSGASTPFLAQLLEKIGLH
                     VKEIYLYDVCSFRDTSQWNEFRELFANKKVDGIVFTSASSVNAFFEIMLMDSDKKTLL
                     ENLQKSKIVAIGPFTADELKKFDVTPLISEVHTVSGAFEKIKNCFN"
     gene            complement(11385..>11796)
                     /locus_tag="VLB45_07290"
     CDS             complement(11385..>11796)
                     /locus_tag="VLB45_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010193611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HSD05533.1"
                     /translation="ELVPGITSGIGSAEYSGIPLTHRDYASSVVFVTGHEDAKKSKES
                     VKWKKLAKSVDTIVIMMGLSRLEIICKNLIGGGLDKKTPVAVIQNGTTPKHRMVKGNL
                     SNISQKVQQAKIRPPSIIIIGKVVNLSEIIGWRK"
BASE COUNT         3273 a         2326 c         2197 g         4000 t
ORIGIN      
        1 cttacgagac gtacctttcc ggtgtttttt tcaagaacca gcagatcatc tccaacaaaa
       61 ctcattgttg taggcaatga gagccctgaa acaaatttct caattactag atcctgatct
      121 tttaaaagcg gatccccata agcaggtaaa agcccagaga aaagtatgag aatcaaaaac
      181 agtatgatct tcaatatttc tttttagcaa ttcacactat taaacagcta aatcagaagg
      241 acagaagatg atcattctac aagaatccaa attgccgttt ttttgcatga cttgactggc
      301 ggaaatattt ctccctttga aacttgaatt accaatttct ctgaacgtgg atgacaacca
      361 atacttccat caacatgcat gacaaaacga taggttccag attgttgaca tatttctccc
      421 gttccaggca caaaattccc taaattctat atgattaaaa tatctctggt tggttaaacg
      481 ctgaatttta tgagaatttg attaaattac aagtaatcaa atggaaaatt caaaacgcaa
      541 tacaaaagtt agaagacaat atttcagggt aaaaagcccc aaaatactgt gcaaatcttt
      601 aggacaaaac acttattatt atttgaacac gaaaaatctc gctgatgact acaataaacc
      661 agcttagaag cggtatgaca agacttgatc caatagaagg aacaattgag agccttggtg
      721 aaccaagaac agtaaatctc agaaccggcg gtcaggcaca agttgctgat gccgttctca
      781 aagacgatac tggccagatc aaactcacac tatgggatgc acaaatcaaa atggtaaaag
      841 ctggttccaa agtgaaagtg gaaaacggtt acatcaacag cttcaagggc gaaaattcac
      901 tcaatgttgg aaaatacggc aagttaaaca taatagaatt ctagaacagt ttagctgata
      961 tatcctaatt ttttatcctg tgccggcagt ggcaattgat cttgcggagg acgctgtccc
     1021 atttgttctt cgattgtttt tatcaggtgt tctgtatctt gtgccctttt ggcaagagta
     1081 gtcatatcta gttttaatcc caacatcttt gcaagaactt ccaagacagc ctttgatgcc
     1141 tttgcatcaa cgacatatcc tgatgtttct cccaacaaac aaactccgtt tattccccga
     1201 gtttttccaa ctccaataac caaaccattc atgccagtta tgcttcctcc gctcattgta
     1261 gaaattccct gctttgaaaa ttcttttact gcatccaacg aagtgcttgt accataaact
     1321 tttggcgtct ttgagaaagt gccagttata taagcagcaa gagtgtagat gtttttgacg
     1381 ccaagttttt cacaaatccc aataatttct tctgccattt catattcacc ttcaggtgta
     1441 actggttgtg catctccggt gagaagtatg atgtcatcag tcttgttttt acaataatac
     1501 aatgtatttt tcatcagatc aactgtacca ttaacttgca ctgagacttg tggagggaat
     1561 gatgatgaga aaatgtttgc aaattgaact cctttaagct cgtctacaag ataatcaact
     1621 gcgagctttc ctacatatcc actacctggc aagccacaga ctagatgtgg attttttaat
     1681 tttggcattt cagaaatttc aatagtgatt tttgatctag acgacatgga ttaggattac
     1741 aacctattca atattaatga agcgtagaga ttctactttc ttctagattt agaccttttt
     1801 gcctttctct ttactttttt tgtttttctt tttgttttcg attttgcctt tgattttctt
     1861 tttgcttttg attttgtctt ttttgttttg atttttcttt ttgtcttcgc tttctttatt
     1921 cttgtcttta ctcttcttgc ttttcttttt gttttcaatt ttgctttttt tgcctttgct
     1981 tttctttttg cttttctggc tttacgttgt gcttttctct ttttagcttt tagtatttcc
     2041 aacctttctg taattttaat cagattacgg tttgcctgtg cttcctgttt taatctagtc
     2101 tgaacttgag aacgcagggc agcctcagct ttagtgctgg attgtagttg ttccaaaagc
     2161 agtggttttg catgcgattg ctgcttcatt tgagaatcaa gttttgactt tgattttgaa
     2221 gtatcagcaa ttagtttaac aagtctttct tctgcagcat ttctttgttt tatctcagag
     2281 tgaagttccg caatcttggc atctatcatt cttatcttct cagatgcacg cgccttgtcc
     2341 tctggggatt ctgaaaattc accttcctgc aaggtcttct cttttgcatc ctcttcttgt
     2401 ttcagtcttg cttctgctgc cagttttagt ctctgtacgc tgtctcgctg tgccatgtat
     2461 tgggtgagca gttgggatat cgattccttt ttttgaaggg cttgttcctt tcttctttcc
     2521 agagaagaca gccctgatga tgatctacgt tttaatgata tagtagaatg aagttttttt
     2581 tcactttgtc gcctgctttt tgttgcctcg attttcttat tcttatacga tgctgcaagc
     2641 tttgagaggg tggtcatggg tttttagaga tttttgactt gattaagttt tgcttatgat
     2701 cagtaattga cataaaagtt aatatttttc acatcttttg tgatttttgt caaattttca
     2761 aaaaatccat tgtcttgacc aactagattt tatttggcaa tacgcaaaaa attgcgtgga
     2821 tttccatccg tcacagctgc caatattgaa aacatttcaa gtggacaacg aatcccaagc
     2881 gtcagaagcg gcagcagaaa tggtcaagat aggattttcg tcacaaaaaa gcgcgttcaa
     2941 agtattgatg acaaatgaaa aggatcttgc caagaagatc ggatttttgg tagttaatga
     3001 aattaatttt ggattacgaa agataaaaca agagcgcgat gtcatgtatt ggatatatca
     3061 ccatgacaaa tcacattatg ccatggtctt gataagctca aaggttttgc acgagttagg
     3121 cttctgattt ttcaaatatt aaatgcagtt tgagtttttt agaaacaatg tttgcaatta
     3181 taattcctag cagtacacca ccaatcacgt ccattggaaa gtgttgtaac acataaaccc
     3241 gacttataga aactatgaga ggatacaccc atacgatata gcatccacgc ggaaacctgt
     3301 gagagagtgc aaatcccatc acaaaggcaa atgctgctgt ccttgtagca tgtcctgaag
     3361 ggaaggaacc gatcccaaaa aatgaagagt cttgctcaag accaatagga aatggagttc
     3421 cttgaaagtc cagttttggc ctttcttggc caacaaaatc ctttacgtat gcggaaacaa
     3481 tcgttcctgc caaaatagaa agtatcagca caagaccaag cctcctggta ctttttttga
     3541 caaacagtat gacacaaaat atcaccatgt agaatacact tccaatttcc gtgaaaattt
     3601 ccattgtcag atcctggatt tgattgcctg caagagattg tgcatatgtt ataactgcat
     3661 catccaaagg ttttgtgatt tttccataaa ctagaattgt gacagctatg aacaatacca
     3721 agagtaagag aaatttttta gaacgaatat caaagatcca ttgttgcatc taacaacacc
     3781 tttgacaatt ttcaagattc atgtctaact aacgctgatt tatctataat aaaatgacat
     3841 cgtatcaaaa tttatgcagt aatcaagaat aaacaataaa tataacaaga aaagacagac
     3901 gggcattgac ataccaagtt atctgcaaag attacggcaa tgaatgtgat tttaaaataa
     3961 gaacggaatc tgaatcagaa ttggttcaaa aagtcaaaaa acacatggaa gacgagcatg
     4021 gcatagattt gccaaaagag cagatcatac agatgagtaa actctaaact gtagaagtca
     4081 gatctcttgt cagactaaaa gattttaacc gtaccagaag ataggatcgc cgtgagactt
     4141 cttacactgg aagatttcga tactaaaggt aagacggtgt ttcttcgtgt tgatatgaat
     4201 tgtcccatag atccaaaaac catggagata cttgagacaa gcagaatacg cgaatccaca
     4261 gaatccctaa aagatcttga gggggcaaaa gtggtcattg catcacacca aggaagagtt
     4321 ggaaacaagg attatactgg aatggaaaag catgcaaaag cactagagaa aatccttggt
     4381 cgcaagatca agtatgtcga ggatgtaatt ggcgcagaag ctcaaagaga aataaaaaac
     4441 atgaagaatg gagagatact tttgcttgat aatctccggc tgtgcgctga agaaaattat
     4501 gaattttctc ctgccgaagc cgcaaaaaca attatggtca caagacttgc caagcttttt
     4561 gacctttgcg tgctggattc ttttccaagc tcacatagat cacacccttc aattgtcgga
     4621 ttttcaagta ttttacctgc ttgtgcagga agactggtag aacgtgaagt tcgcaagcta
     4681 gatgagataa tgacagttgc taagggagca catgtcgtag tgcttggagg ctccaaggtt
     4741 gctgacagac tagaagcaat gaaaacactg attcaaaatg gcagagcgga tcaaatcttg
     4801 ctgaccggag tcattgccaa tgttttcatg cgtgcacaag gcagaataaa atttccgctt
     4861 gggataaaaa gggaggaaga aatagtttca aaagctcatt ctcttatcgg ggaataccca
     4921 gatgtttttt cgacacccgt agatgttgca actgacaggg gcggtgtgag agttgagatg
     4981 gatgtgagag agcttgacaa gggagaccag atacttgatc ttggtccaaa gactgtagag
     5041 cattatacaa aatcaataca gagtgcagga accgtattta tgagtgggcc agcaggattt
     5101 tttgaaaatg acaattttaa gtttggaaca gaaagtcttc taaaagcagt agccaattct
     5161 tttgcaacca ccattgtaag tggaggccat cttactgcag cgttgaaaag atatggcttg
     5221 acagaaaaaa taaaccacat cagcacagca ggcggcgcac tagtgttgta tttgacagga
     5281 gcaaagttgc ccatgattca aacacttgaa gaagcagcag aaaaatacag aactaaatag
     5341 ctgctttaca tgcatggttt gggcatcttt tttcttccaa tgtgcccaga tagatttcaa
     5401 gattgcatga attacatttt gatttttcta ccagtccaaa cagcttgaac cactgggttg
     5461 taaagtcttg tgctattggc ctgagcagtc cttcctcatg tagttggtta aagaaaggtt
     5521 tcatatcatc aattgttaac gatttggcac catcttggtt ctttaatatt tcaaacacct
     5581 catcatttgt aaatctgaga tttacatcat tgaatttttc aaaaattatt tttcgtactt
     5641 gtaaaagtat tgatgcttga ttgtctgtca acttagaaca gaccggatgc ttcttctttt
     5701 atcttatcgg cagaggcaac atcatttagc ttttctgata cgtatgagta tatgccatat
     5761 ttgagaacct tgagagaaag gagtgcacac tttattctgg caggtccaag atcaggtaag
     5821 cctaggtttt caagaatgtc ttcttttgtc agttttttaa cataatcgaa atcttttccc
     5881 atgatgagct ccgtaagcat ggatgcgctt gcctggctta ttgcacagcc tttgcctgtg
     5941 aattttacat cagacacttt gttgtccttt aggttgatgt gcatttcaag ctcgtcacca
     6001 cacagtggat tgctgtcccg ttttgcaatg tctggatttt ctatttttcc atagtttcgt
     6061 ggatttctgt aatagtccag aatgatttcc cggtaaatat ctgcgctact caaattctaa
     6121 acaactctct tgctctatta agtgacctga taaaaacatc cacttcctcc tttgtattat
     6181 aaatataaaa gcttgcgcgg gatgttgcag atacatccag tctttccatc aacacttgtg
     6241 cacagtgatg acctgaacgt atagcaattc catcttcatc aattatagtt gcaagatcat
     6301 gcggatgtat gtcaccaaga ttgaaggaaa tcactcctcc ccttttttta acatctgacg
     6361 gtccatatag tatgattccc tttattgcag aaattttgtc tagagcatat tttgtcaatt
     6421 caatttcatg ttctcttact ttatccatgc caatgttctc aagatagtct atagctgcag
     6481 aaaatccaat tacatcagcg atgtttggtg tgccagcttc aaacttgtat ggcaggtcat
     6541 tccagcttgt ctcatacttg tgaacctccc ttatcatgtc gcctccgcca ttgaatggtt
     6601 gcatgttttc taggatttct tttcgtaccc ataacactcc tactcctgta ggtcccaaca
     6661 tcttgtgagc ggaaaatgca aaaaagtcac agtctagttt ttgcaggtca actttcatgt
     6721 gtggaacaga ttgggctgca tcaactagca ctttgactcc ctttgcgtgg catcttttta
     6781 tgatttcctc ggttggagaa attgttccca aaacatttga catctgacta aaagtaacaa
     6841 gctttacttt tcctgtttcc agataggcat caagatgatc gaggattagt tccccgttgt
     6901 cgtctactcc aatgtattcc agtttcgctc ccttttcttt tgtaagcagc tgccatggta
     6961 cgatattact gtgatgctcg tattctgttg ttacgataat gtcatctttt tgaacatttt
     7021 gtcttcccca tgaatatgca acaagattta tcgcttctgt tgttcccctt acaaagataa
     7081 tttcttcgcg attttttgcg ttgataaatt ttgtgacttt gtctcttgtc atttcatatg
     7141 ctgcagttgc ttcttctgca agctggtgta ctgctctgtg tatgttagaa ttgtaattca
     7201 gataataatt atgaatagag tctatcactt ggattggctt ttgtgttgtt gccgcattat
     7261 caaaatacac aagaggtttg tctttgtgca ccttgcgctg tagaattgga aaatcctttc
     7321 taatattttc aatatttatt gtgcttgctt gcaagattta ctttacctcc acatagattt
     7381 cattttgttc tattttaaca ttgtaggttt taagcggttt ttcagctggc agattttgcg
     7441 gcatgccagt ctcaaggttg aaagtagaaa gatgcaatgg acatgttact ccttcttcac
     7501 ctaaaattcc tgctgaaaga tcagcttctg catgtgtgca gattctatct gttgcataaa
     7561 tttttccatt catatttgca agcataatct ttttgtcatt atgatcaaaa ctgtataact
     7621 ctccttttcc aagttttttt gtactacaaa cgtttaccca ttcagacaag atgctcacct
     7681 gtatttgtag tggctttcga atatgtcagt ttcacggtat cttgtttctt ccacttcaag
     7741 tattgccttt agctgttcgt ctgtttttat tgtaagatct ttgcctagcc acttgcagtc
     7801 tataagatag tttatccatg cacgtatctg gtatgacatt tttcttgaaa gcggttcaag
     7861 aaatccctct acgattattt tctgtgcttc tgatttgtca aggcatcttg agccaagata
     7921 gaagagttgt tcgtcgtcaa gctgggcaac tgatgcagcg tgcgttgcct tgacatcatt
     7981 tgtaaaaatt tcaagtcctg gtatcgagtc agattttgca cccttgtcaa gcaaaattga
     8041 atggcccgca agataagatt ctgaatggtg tgcatgttct tcaattcgta tcattccttt
     8101 gaatagtgat tttgcggtat cttttaggac tgacttttca agaactcttc ctgtggtaga
     8161 tggtgctaca tgtatcaagt ttgacgtaat gtcaaatgac tggtttttgt ttccaaacac
     8221 aacctcagta tcgtttgcag ttgcgccaat ctcatttagg taattatcaa ttttatatct
     8281 agaaagtaat gccccaaaca atccaaggta ccaattcatt cttgcgtctc gtgcaatttt
     8341 tcctgttctg gtagagaagt ttattgcatt ttggtccatg gcttgcaacg taaccatgtc
     8401 aagttcgctg ttttgtctca cgctaacatc aagcaactca agatatgcct gctgttttgt
     8461 gttctttggc gcataaagct cctgaacgat cattgccttg ctgttttctt ctgcaaccac
     8521 aatgttgcgc gacattgtag aggtaccatc cacagaaaga gatgaaattg catgaatcgg
     8581 tttgtccaaa atcagatttt ttggaacata aatgaaaaat cccgagttga aaaatgcgtt
     8641 gtttagtgcg atgtatttgt cctttttagg atcagtaaga tccaaggatt ttttgacaat
     8701 gtcagaatga ttctttattg catcatggat tgagcagatg atcaatcctt ttgatttcag
     8761 ttcagatggc agatttatct ggtggatgtt tgtcccgatt tggacaatgc ttgcgccatt
     8821 tgctatctct gccattcttt cttttacaaa atctggaact gtgctgtctg agcttagcga
     8881 aaatgaaatc ttttctggat ccattttgtt tgcgtcagag tatttgttgt atagcggcga
     8941 gacttcgtat ggcagctcct tgtagattga aaaggaattt tccctgtatt ctttaagcca
     9001 tgccggttca ttatttgcag atgaaatttc gtctacatga ttttgtccaa gtgctgatag
     9061 gagaaacgat gtcatgcttg tcacacaaat atgatggaac ccagtctaac cgactgagcc
     9121 atccatttcc aattttacta gcctattgag ctctactgca tactccatag gaagttcttt
     9181 ggtaaacggc tcaatgaacc catttacgat cagagtcagt gcatcttctt ctgaaaatcc
     9241 tctgctcata aggtagaaga tttgttcatc gcctattttt cctacagtcg cctcgtgggt
     9301 tactgtagca tcttcttggt taatctccat gtacggatag gtatcggttt ttgaagtgtc
     9361 atctaaaagc aatgcgtcac atcttacgga agcctttacg tttgtggcac cttttgcaac
     9421 gtgcaataat cctctgtatg tcgttcttcc atttgctcgg cttactgatt ttgatgtgat
     9481 tcttgatgta gtatttggcg ccaagtgtac catctttgca cctgtgtctt gatgctggtt
     9541 ctttccagca aatgcaacag acaatgtttc accatgtgct cttggtccca ttaaataaat
     9601 tcctgggtat ttcatggtca gcttgcttcc aatgtttccg tcaatccatt ctactcttgc
     9661 gccttcgtaa gcataagctc gttttgtaac tagattgtaa acgtcattgc tccagttctg
     9721 aattgtagtg tatctgagtt ttgcgccctt taatgcaatc aactctacta ctgcagagtg
     9781 taatgactct gaagaatata ctggtgctgt gcatccctcg atgtaatgca cttctgatcc
     9841 ttcgtctgca ataattagtg ttctttcaaa ttgaccaatg ttttctgcat tgattctaaa
     9901 gtatgcttgt agtggaaggt ctactttaac gccaggtgga atgtaaatga atgatccacc
     9961 actccagact gcgctgttta gtgcagcaaa tttgttgtct tctggaggaa tgattttacc
    10021 aaaatatttc ctgaaaattt ctggctcttc ttttagagcg gtatcagtat caaggaactt
    10081 tactccttgt tttgcaaggt cttctcttag attatgataa acaacttctg attcatactg
    10141 agctcctacg ccagcaagaa atttcttttc agcctctgga attccaagct tgtcaaatgt
    10201 gtttttgacg tcttctggaa cattatccca gctcttctct gtcttttcag aagcctttgc
    10261 ataatagtag atattgttaa agtcaatttt gctcagatct cctccccagt ttggcattgg
    10321 ttttttcatg aaaatgtcat atgatctcag tctaaagtca agcatccact gtggttcgcc
    10381 ttttagtttg cttatttctt caactgttcc gcgggaaaga cctttcttgc tgagataaac
    10441 atacatctca gtagagtcct tgaaatcata tttactataa tccatattga ggtcttcagt
    10501 tgccatctgc tttcataacc ccgttatatt ataaatagct tatacaactc atactagttg
    10561 tacagcggag taggtcaagc taaataggcc aagctaattg aaacagtttt ttattttttc
    10621 aaaagctcct gatactgtgt gaacttctga aatcagcgga gtgacatcaa attttttcaa
    10681 ttcgtcagct gtaaatggtc caattgcaac aatttttgat ttttgtaaat tttccagcag
    10741 tgttttttta tcagaatcca tcaacattat ctcaaaaaat gcatttactg atgatgcgct
    10801 tgtaaataca atgccatcaa cttttttgtt tgcaaaaagt tctctaaact cattccattg
    10861 tgatgtatct ctaaacgaac acacgtcgta aagatagatc tcttttacat gcagacctat
    10921 tttttcaaga agttgtgcaa gaaacggagt agatgcacca cttcgtggaa tgatggcttt
    10981 ttttccttcc gcatttaacc ttgtaaaaac ttcgcctact cccaaagaag agtatctttg
    11041 tggcatgtgg gcaaccctta taccctcagc ttcaagcgct gtctttgtct ttggcccaac
    11101 tgcaatcaca ttggtatttg caacagccag ttgaagcttt tcatattttg aaacttttct
    11161 tgcagtatca aacaagagtt ttaccgcctt tgagctcata aagacagaaa aatcaggatc
    11221 atcttgcttt attgcatcca aaaattcgtc tactatctta tcgcctttgc taacaagttc
    11281 gattgttgga agcggtattg cccttgcgct tgcctttgtt atcaattgaa caaattcttc
    11341 cgagtcttcc tttgagcgcg taattgctat agtttttccc gtaatcattt tctccaacct
    11401 attatttctg agagattcac taccttacct attataatta tagagggcgg tctgatcttt
    11461 gcctgctgta ctttttgaga tatgttggaa aggtttccct ttaccatcct gtgctttggt
    11521 gtggtaccat tttgtatcac agctacaggt gtttttttgt ccaatccacc accaattagg
    11581 tttttgcaga ttatctcaag ccttgaaaga cccatcatta ttactatagt atcaacagac
    11641 tttgcaagtt ttttccattt cacggactct ttggattttt tagcatcctc gtgtcccgta
    11701 acaaagacga cagaagatgc ataatctctg tgagtaagtg gaattccaga atattctgca
    11761 gaaccaattc cagatgttat tcctggaact agttca
//
LOCUS       DATIDL010000158         4359 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrosopumilaceae archaeon isolate SMAG_U16342
            k141_2014364, whole genome shotgun sequence.
ACCESSION   DATIDL010000158 DATIDL010000000
VERSION     DATIDL010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935836
            Sequence Read Archive: SRR25158326
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosopumilaceae archaeon (soil metagenome)
  ORGANISM  Nitrosopumilaceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrosopumilales;
            Nitrosopumilaceae.
REFERENCE   1  (bases 1 to 4359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/04/2024 05:09:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,520
            CDSs (total)                 :: 1,474
            Genes (coding)               :: 1,474
            CDSs (with protein)          :: 1,474
            Genes (RNA)                  :: 46
            rRNAs                        :: 1 (5S)
            complete rRNAs               :: 1 (5S)
            tRNAs                        :: 43
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4359
                     /organism="Nitrosopumilaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2014364"
                     /isolate="SMAG_U16342"
                     /isolation_source="soil"
                     /db_xref="taxon:2202732"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..115
                     /locus_tag="VLB45_07295"
     CDS             <1..115
                     /locus_tag="VLB45_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007551563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSD05534.1"
                     /translation="IIVTHDRQQFGDVDRIITIKDGRAFEGTEQKGMEVAV"
     gene            112..1413
                     /locus_tag="VLB45_07300"
     CDS             112..1413
                     /locus_tag="VLB45_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012216094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05535.1"
                     /translation="MMRKLSMLFVIFFIGQLFVINAYGQFTKSDSPFEREFADIKFLD
                     AYFGTFEQKVEVDAGDQNVPLTVVMANIGTQDITGIRGQLSLPSGFSPSDGKDALILA
                     DKDDNSLAGDVFSLTFFVNVDKNLDLGQYPGTIKVDYSRLRETGERNIFFNFDFKLTG
                     DSTLSIKAIDPILTSIKNNPVVVEVSNLGTAPISNIDIVLQNTQTSISSTTQSVTNLE
                     NVVFDQNTWDIGTIEPKSSKHFSFNVYVPENLKDESLHLPVEITYYNAHGDTNTITKT
                     IDFYINGLISATIYNVDVIELSKKQTVIGEILNEGNVDGLFAFVTLEPRGDSNIKKST
                     QYIDKLEPDSPVPFNIPIEFDGEPREGEHDVRITTKYKDSLRNENTITYDTTVFYKDT
                     SKDESGLPDASLFIILGIVAVAGVFAYYRIKKRKKTPTQTS"
     gene            1444..1836
                     /locus_tag="VLB45_07305"
     CDS             1444..1836
                     /locus_tag="VLB45_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05536.1"
                     /translation="MTWDEIEVPKEVRPFMLEEAEETRLGQKNGANKQYRYGNLHIRE
                     YDDKFLIHMDKVDPRKNPLGHLVFDAPEVLIGLATAAIGGKKVASEIYKLQKNTKHAK
                     HVSLVTGFLASIALGYVGYSLTKKAKDL"
     gene            1889..3403
                     /locus_tag="VLB45_07310"
     CDS             1889..3403
                     /locus_tag="VLB45_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HSD05537.1"
                     /translation="MPLILTLRKDRREWVRKEGKNVNTKRYQKNAKKILNTFVSLGPV
                     YIKLGQWLSSRADILPQPYMEELAKLQDDVPAAPFDQVRPIIENELGPIEQNFDYINT
                     NVLSGASLGQVYLAKIKEKDVIIKVRRPNIDKIIEEDIRILKKFLPIGMKFVDPNLRY
                     SAEAMLSQFIETINEEMDYTIESQNLKTIKRNLQNYPKVIIPSVIDDHSSKQVLTLEY
                     IPGIKITNIKALDEAGIDREQIVIKTHKVFFTMLLHHNIFHADPHPGNIAVTNDGSLI
                     LYDFGMVGRLDNETRLKLIRLYLSLVEKDPPRTVNAMNDLGMLIPGFNHGIIEKGIDM
                     SIQAMHGKKVDNMEVKALMELANKTMSKFPFKLPKHLALYMRMASILEGIYLTHKVKF
                     RFIKVLQNILEEENLIRDAYVEELKYSLNKFAKSIDATISIAPELKRYLDDNKSLQNL
                     TQKPFGSILLSGSILSAAVFVGSTVLYTVNESVGQIGMIGSIIIMGVSVFLRKH"
     gene            complement(3405..3788)
                     /gene="hsp14"
                     /locus_tag="VLB45_07315"
     CDS             complement(3405..3788)
                     /gene="hsp14"
                     /locus_tag="VLB45_07315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012216090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaeal heat shock protein Hsp14"
                     /protein_id="HSD05538.1"
                     /translation="MGLVNYMAKEILKEIGNKSREFYEFVLPPVDMLLENNNLIVTID
                     IPGFDKKDIKLRINGNILSIEAKRETDHDGTIIWHQRPHVIDKKIRLPIDVKEGEDAV
                     GSAKYLHGVLTLKIPMKSGKDITIE"
     gene            3866..>4359
                     /gene="tmk"
                     /locus_tag="VLB45_07320"
     CDS             3866..>4359
                     /gene="tmk"
                     /locus_tag="VLB45_07320"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HSD05539.1"
                     /translation="MIIVLEGFDQAGKGTQSKLLAKYLKSKKLKYKIFSFPDYATPIG
                     KEIQNYLGGKRKFPPQVIHCLLAANRWEKIGEIKDALAKNYVLIMNRYYQSNLVYGIT
                     NGLDLKWLLNLDTGLPKEDLVIVLDVKPKESFNRKKIQRDKFEKNKKFAQKIVGTYRS
                     LAKD"
BASE COUNT         1587 a          757 c          758 g         1257 t
ORIGIN      
        1 cattatagtt acacatgaca gacaacaatt tggtgatgta gaccgaatca tcaccataaa
       61 agatggtcgt gcttttgagg gaacagaaca aaaaggaatg gaggtagctg tatgatgaga
      121 aaattatcta tgctgtttgt gatatttttc attggacagc tttttgtcat aaatgcatat
      181 ggacaattta ctaaatcaga ctctccattt gaaagagaat ttgctgacat caagtttctt
      241 gatgcatatt ttggcacttt tgagcaaaaa gtcgaagtgg atgcaggaga tcagaatgtg
      301 ccactaactg tagttatggc aaatataggc acacaggata ttacgggaat aagaggacag
      361 ttgtcattac catctgggtt cagtccatca gatggcaaag atgctctaat tctagcagat
      421 aaagacgata attcccttgc aggtgatgta ttttcactaa cgttttttgt taacgtagac
      481 aagaatttgg atcttggaca atatccagga acaataaaag tagattattc tagactaaga
      541 gaaactggag aaagaaatat ttttttcaat tttgatttta aacttacagg agacagtact
      601 ctaagcatta aagcaataga tccaatactc acatcaataa aaaataatcc tgtagttgtt
      661 gaagtttcta acttgggcac tgcaccaata tcaaatattg acatagtttt gcaaaatacc
      721 caaactagta tctcatcaac tactcaatca gttaccaact tggaaaatgt tgtatttgat
      781 caaaacacat gggatattgg aacaatagaa ccaaaatcaa gtaaacattt ctcatttaat
      841 gtgtatgtgc cagagaatct aaaagatgag tcgcttcatc taccagtgga aataacatac
      901 tataatgcac atggtgacac aaatacaatc acaaaaacca ttgactttta cattaatgga
      961 ctaattagcg cgacaatcta taatgtcgat gtaatagaac tgtcaaaaaa acaaactgtt
     1021 atcggtgaaa ttcttaacga aggtaatgtg gatggtctgt ttgcatttgt tacactcgaa
     1081 cctcgtggtg actctaacat aaaaaaatca actcaatata tagacaaact agaacctgat
     1141 tcaccggtac cattcaacat tccaattgaa tttgatggcg agccaagaga aggagaacat
     1201 gatgtacgta tcactacaaa atacaaggat tctttacgaa atgaaaatac aattacctat
     1261 gatactacag ttttctataa ggatacatca aaagatgaat ctggtttacc tgatgcttct
     1321 ctattcataa ttttgggcat agttgctgta gctggcgtgt ttgcatacta tagaatcaaa
     1381 aagagaaaaa aaacaccaac ccaaacaagc taaagttata tttagtgtaa ggaatttttg
     1441 cttatgactt gggatgaaat tgaggtccca aaagaagtta gaccttttat gcttgaagaa
     1501 gccgaagaaa caagattagg tcagaaaaat ggtgccaaca aacaatatcg ctatggaaat
     1561 ttacacattc gagaatatga cgacaaattc ctgattcata tggataaagt tgatccacgc
     1621 aagaatccac taggtcatct tgtctttgac gcaccagaag ttctcatcgg ccttgcaaca
     1681 gcagctattg gaggaaaaaa ggtcgcttca gaaatttaca aattacaaaa aaataccaag
     1741 catgcaaaac atgtttcact ggttacagga tttttagcat cgatagcact tggatacgta
     1801 ggctattctc tcacaaaaaa ggccaaagat ctctaggaag gaatctgtca tatcaaaaac
     1861 aagaaccgtt aagatctttc tcaaactttt gccactgata ttaacactca gaaaagatag
     1921 aagagaatgg gtcagaaaag aaggaaaaaa tgtaaacacc aaaagatatc aaaagaacgc
     1981 aaaaaaaatt cttaatactt ttgtatcact aggaccagtt tacatcaaac ttggtcagtg
     2041 gctttcatca agagctgata tcttaccaca accatacatg gaggagcttg caaaattaca
     2101 agatgacgta cctgcagctc catttgatca agttagacca ataatagaaa atgagcttgg
     2161 accaatcgaa caaaactttg attacataaa cacaaacgtt ctttccggcg catcacttgg
     2221 gcaagtctac cttgctaaaa taaaagaaaa agatgtcata atcaaagtaa gaagaccaaa
     2281 catcgataaa atcatagaag aggacatcag aattctcaaa aagtttcttc ctattggaat
     2341 gaagtttgta gatccaaatt tgcgttattc tgcagaggca atgctttcac agtttattga
     2401 aactattaac gaagagatgg attatacaat tgaatctcaa aacctaaaga ccataaaaag
     2461 aaaccttcag aattatccaa aagtgataat tccttctgta atagatgatc actcttcaaa
     2521 acaagtactc acgttggagt acattccagg aatcaaaatt acaaacataa aggcacttga
     2581 tgaagcagga atagacaggg aacaaatcgt aatcaagacg cacaaggtgt tttttacgat
     2641 gcttttacat cacaacatat ttcacgcaga tcctcaccct ggcaacattg cggtaacaaa
     2701 cgatggttcc ctaatactct atgattttgg catggttgga agactggaca atgaaaccag
     2761 actcaaactc atcagattgt atctctcgct tgttgaaaag gatcctccaa gaacagtgaa
     2821 tgcaatgaat gatcttggaa tgctgattcc tggttttaat catggaataa ttgaaaaagg
     2881 aatcgacatg tcgattcagg cgatgcatgg aaaaaaagtg gataacatgg aagtcaaagc
     2941 actgatggag ctagcaaaca agacaatgag taaatttcca ttcaagcttc caaaacacct
     3001 agcactttac atgagaatgg catcaattct tgaaggaatc tatctcacac acaaggtaaa
     3061 attcagattc atcaaggttt tacaaaacat tttggaggag gaaaacctga tcagagatgc
     3121 atatgttgaa gagctaaaat attctctcaa caaatttgca aaatcaattg atgctaccat
     3181 atcaattgca ccagagctga aacgatatct tgatgacaac aaatccttgc agaatctaac
     3241 acagaaacca tttggaagta tattactttc aggaagcatt ctgtcagcgg cagtttttgt
     3301 tggttcaaca gttttgtata ctgtgaatga atctgtagga caaattggta tgataggttc
     3361 tataattata atgggagttt ctgtcttcct aagaaaacac tgaattactc tattgttatg
     3421 tcttttccac ttttcatagg aatcttcaga gttagaaccc catgaagata ttttgcagaa
     3481 ccaacagcat cctctccttc ttttacatcg attggtaatc taattttttt atcaattaca
     3541 tgcggtcttt gatgccaaat tatggttccg tcatgatctg tttctctttt agcttctatg
     3601 gaaagaatgt ttccgttaat tctcagcttg atgtcttttt tatcaaaacc tggtatgtct
     3661 attgtaacaa tcaaattgtt attttctaat agcatgtcca caggtggaag tacgaattca
     3721 taaaattctc gtgatttgtt gccaatttct ttcaagattt cttttgccat atagtttacc
     3781 aagcccattt aaagtaataa aggattttta agttataaac cttgatagat tctaatttat
     3841 ctagttctca attaattaca atttcatgat aatagttcta gagggatttg atcaagcagg
     3901 taaaggaaca caatcaaagc ttctcgccaa atatttgaaa tcaaaaaaac taaaatacaa
     3961 aattttcagt tttcctgatt atgccacacc gattggaaaa gagatccaaa attaccttgg
     4021 tggaaaaaga aagttcccgc cccaagtgat tcattgcctt cttgctgcaa atagatggga
     4081 aaagataggg gaaataaaag atgcacttgc aaaaaattat gttctaataa tgaacagata
     4141 ctaccaatca aatctagttt atggaataac taatggtctt gatctaaaat ggcttttgaa
     4201 tctagacact ggacttccaa aagaagatct tgttatagtt cttgacgtca aaccaaaaga
     4261 gtcatttaac cgcaaaaaaa ttcagcgtga taaatttgaa aaaaataaaa aatttgcaca
     4321 aaaaatcgtt ggtacttatc gatcactagc aaaagactt
//
LOCUS       DATIDL010000159         2357 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrosopumilaceae archaeon isolate SMAG_U16342
            k141_2420986, whole genome shotgun sequence.
ACCESSION   DATIDL010000159 DATIDL010000000
VERSION     DATIDL010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935836
            Sequence Read Archive: SRR25158326
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosopumilaceae archaeon (soil metagenome)
  ORGANISM  Nitrosopumilaceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrosopumilales;
            Nitrosopumilaceae.
REFERENCE   1  (bases 1 to 2357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/04/2024 05:09:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,520
            CDSs (total)                 :: 1,474
            Genes (coding)               :: 1,474
            CDSs (with protein)          :: 1,474
            Genes (RNA)                  :: 46
            rRNAs                        :: 1 (5S)
            complete rRNAs               :: 1 (5S)
            tRNAs                        :: 43
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2357
                     /organism="Nitrosopumilaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2420986"
                     /isolate="SMAG_U16342"
                     /isolation_source="soil"
                     /db_xref="taxon:2202732"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            165..464
                     /locus_tag="VLB45_07325"
     CDS             165..464
                     /locus_tag="VLB45_07325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05540.1"
                     /translation="MASLSYDDYVNNAVADVSVISALDQPIQSGTTYVTGSENHVLST
                     QFPSIPTGITVTENHSNLVNAPNRLVFESLWIPLLIIGSYILVHTDYAVLSGVKQ"
     gene            694..1659
                     /locus_tag="VLB45_07330"
     CDS             694..1659
                     /locus_tag="VLB45_07330"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010191877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HSD05541.1"
                     /translation="MTANGIMSYQDEVRVYLDPKHGTKDGISFVSHAHIDHLHNQNNG
                     LLLTTKQTSEIAKLRGYNINNFVEEFEDFSMVDAGHILGARGLLFGDEIFYTGDICTR
                     TRGFLKGAQVPKCRTLIMECTFGLSEFVFPSIGETVKRVNGIISEMYSKGKPVILLGY
                     ELGKAQTLSYLFGHWDPLYYHDSVKKINDLHRSFGINIKDTMGHTEAEKNGLLEKKPW
                     VMIAPNMSGKSQFVNYMKSKYDAITIGFSGWAQSGRFSFARQHDYSIALSDHCDYNEL
                     VDLVKKSNPEKVYTVHGFTDEFANDLLKMGFDAQPLREDSLDEYL"
     gene            complement(1676..1966)
                     /locus_tag="VLB45_07335"
     CDS             complement(1676..1966)
                     /locus_tag="VLB45_07335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05542.1"
                     /translation="MQQLEERSSLLDAIVNMNDGVGLSSHDIKSDKPEHVESIRYESF
                     ALLDHLSVIKMEIDIIKERTKHVVDKKTQTQFSRIDKEIAKMTEHLIELRPQ"
     gene            complement(2057..>2357)
                     /locus_tag="VLB45_07340"
     CDS             complement(2057..>2357)
                     /locus_tag="VLB45_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008299345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="excinuclease ABC subunit C"
                     /protein_id="HSD05543.1"
                     /translation="EIIKRRYLKLRIEKDKMPDLILVDGGKGQLRAALDALKNVGTDI
                     PCVSLAKENEEIYLPKYKEPVIIPKNNQGLKILQHARDEAHRFGVAYNRSLRKFS"
BASE COUNT          714 a          418 c          424 g          801 t
ORIGIN      
        1 agcttaggtt caatttttta ccctaattct gtctgtaatt tttcttactt acttcttctt
       61 ataatgaaaa atatcgttct tgtgaatagt tgattaaatc tatcacagtt catcacacac
      121 gtataatttt cggtataacc ctctgtctag taggattagg atttttggca tctctttcat
      181 atgatgacta tgtgaataat gctgttgccg atgtcagcgt gatatcagca ctagatcaac
      241 ctattcagtc tggcacaaca tacgtaaccg gatctgaaaa tcacgttcta tcaacacaat
      301 ttccatccat tcctactggt atcactgtaa ctgaaaatca ttcaaactta gttaatgcgc
      361 caaatagatt ggtctttgaa tctctatgga ttcctctgct cattataggt agctacattc
      421 tagttcacac tgattatgct gttctttcag gggtaaaaca ataatgttcg tcgctatgga
      481 atgcaagtca agcgaacatc atgcctgcca tacaattcca tgtgactgcc catgtcatac
      541 tagaaaaatg aatcatgatc gatggaaaaa ctaggctcaa agaacttaca aactaatttc
      601 taacctgaca ttgactagac actaactgcg cattaatctt gtaatttata tctgattttg
      661 tgtttttgaa tcggattggc atcatgcatt aaaatgaccg caaatggaat tatgtcatat
      721 caggatgagg tacgcgttta tcttgatcca aaacatggca caaaagatgg catttccttt
      781 gtttcgcatg cacatataga tcatttacat aatcaaaaca acgggttact tttaactaca
      841 aaacaaacaa gtgagatcgc aaagcttcgt ggatacaata ttaataattt tgtagaagag
      901 tttgaagatt tttcaatggt agatgctgga cacattcttg gagcacgtgg actattgttt
      961 ggtgatgaaa tattttacac tggggatata tgtacaagaa caagaggatt tctaaaagga
     1021 gctcaagttc caaaatgtag aacgcttatc atggaatgca cgtttggatt atcagagttt
     1081 gtttttccaa gcataggtga gaccgtaaaa agagtgaatg gaataatctc tgaaatgtat
     1141 tctaagggaa aaccggttat cctacttggc tatgaacttg ggaaagccca aacactttcg
     1201 tatctatttg gtcattggga tccgctttac tatcatgatt ctgtgaaaaa aatcaatgat
     1261 ttacacagat cgtttggaat taatataaaa gacaccatgg ggcacaccga ggctgaaaaa
     1321 aatggtttgt tagaaaagaa gccttgggta atgattgcgc ctaatatgag tggaaaaagc
     1381 caatttgtta attatatgaa atcaaaatat gatgcaataa caatcggctt tagtggttgg
     1441 gcgcaatcgg gacgattttc atttgctaga cagcatgatt attcaattgc cctaagtgat
     1501 cactgtgatt ataacgaact tgttgatcta gtaaaaaaat caaatcctga aaaagtgtat
     1561 acagtgcatg gattcacaga tgaatttgca aacgatctct taaaaatggg atttgatgct
     1621 cagccattac gcgaggattc acttgatgaa tacttgtaat ttaatttttg atatcttatt
     1681 gtggtcttag ctcaataaga tgctctgtca tctttgcgat ttccttgtcg attctagaaa
     1741 actgtgtctg tgtttttttg tctaccacat gctttgttct ttcttttatg atatcaattt
     1801 ccatctttat gacagatagg tgatctagaa gagcaaatga ttcgtatctg atggattcaa
     1861 catgttctgg tttgtcagat tttatatcat gtgatgacaa tcctactcca tcgttcatat
     1921 ttacgatcgc atccaagaga ctagatcgct cttctagttg ttgcatgtgc ttcatttcaa
     1981 gtgtaaatat aaaaaactgg tctgccaaaa atggaaacaa ggtggcttat ttataaacca
     2041 atgattactg ttgcaactaa ctaaacttgc gtagtgatcg attatacgca actccaaacc
     2101 tatgtgcctc atctcttgca tgttgtaaaa tttttaatcc ttggttattt tttggaatta
     2161 ttactggttc tttgtatttc ggtagataaa tttcctcatt ttcttttgca agcgaaacac
     2221 acggtatgtc tgtccctaca ttttttagtg catcaagtgc ggccctcagt tgtcctttcc
     2281 caccatctac aagtatcaaa tctggcatct tgtctttttc tattcgtagc ttaagatagc
     2341 gtcgttttat tatttca
//
LOCUS       DATIDL010000160        29412 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrosopumilaceae archaeon isolate SMAG_U16342
            k141_2217477, whole genome shotgun sequence.
ACCESSION   DATIDL010000160 DATIDL010000000
VERSION     DATIDL010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935836
            Sequence Read Archive: SRR25158326
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosopumilaceae archaeon (soil metagenome)
  ORGANISM  Nitrosopumilaceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrosopumilales;
            Nitrosopumilaceae.
REFERENCE   1  (bases 1 to 29412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29412)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/04/2024 05:09:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,520
            CDSs (total)                 :: 1,474
            Genes (coding)               :: 1,474
            CDSs (with protein)          :: 1,474
            Genes (RNA)                  :: 46
            rRNAs                        :: 1 (5S)
            complete rRNAs               :: 1 (5S)
            tRNAs                        :: 43
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29412
                     /organism="Nitrosopumilaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2217477"
                     /isolate="SMAG_U16342"
                     /isolation_source="soil"
                     /db_xref="taxon:2202732"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            81..704
                     /locus_tag="VLB45_07345"
     CDS             81..704
                     /locus_tag="VLB45_07345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05544.1"
                     /translation="MLLKNHDNDVTKNVVLDLDDILSQSKINSVLYSDPFLKAGFLAK
                     LVQDTKINVLYLDLDLLYSGYLASEILGTPQNLVLFRPTLELWNQMLTDVLVKLSNKK
                     SLVVIDSLNGLYNILNERKEVGKLVTSYVMLITSMAKITNSYIVVASMVRFKKEEGWV
                     LSPTGKRLIETSMAKKILLDQNDSEITLDFLKEKKKITIPLNAIPLV"
     gene            complement(696..971)
                     /locus_tag="VLB45_07350"
     CDS             complement(696..971)
                     /locus_tag="VLB45_07350"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008298768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HSD05545.1"
                     /translation="MGNKVHCAHILVEKLSLAQELKTKIVKGESFANLAKQFSIDGSK
                     RRGGDLGFFGRGMMVPEFEKAAFSLEKGQVSDIVKTQFGYHIIKRLD"
     gene            1186..1485
                     /locus_tag="VLB45_07355"
     CDS             1186..1485
                     /locus_tag="VLB45_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HSD05546.1"
                     /translation="MPVGIIPDIGEQMCIGCALCVEICTSLGPDVLRVKPVEGWKRGK
                     AFVFYPERCISDGACIGVCPTKAIFWMRPMDFTVGQPVPLYRNSVFVKGWTELAE"
     gene            1586..2080
                     /locus_tag="VLB45_07360"
     CDS             1586..2080
                     /locus_tag="VLB45_07360"
                     /EC_number="3.1.3.16"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010189452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dual specificity protein phosphatase 23"
                     /protein_id="HSD05547.1"
                     /translation="MSKPGNLWRKVHGRLTKKPTNFSWLINNKLAGSGMPTSLAELDW
                     VFKQGVKSIITMTEESLPESWIKDVRYLHVPTEDLSAPDIEKIDLAVDFIQERIKNNE
                     AIMVHCAAGIGRTGTILACYLIKYQNLSVKTAIDKVRQERPGSIQSESQEIAIGLYHK
                     FINK"
     gene            complement(2081..2236)
                     /locus_tag="VLB45_07365"
     CDS             complement(2081..2236)
                     /locus_tag="VLB45_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05548.1"
                     /translation="MAKFDGIRGQELLEVEDKSEKENEITLIFKDNRYLFIKLENGKM
                     VTSSIPE"
     gene            complement(2269..2811)
                     /locus_tag="VLB45_07370"
     CDS             complement(2269..2811)
                     /locus_tag="VLB45_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05549.1"
                     /translation="MTSEKSFIGNVELVKPNDKFKKDDNVVINVKFSVLGALRESWNE
                     KNWEKAYSKHDNAFKIKYGIKLFSGGLRKHELGKPVDTYRKAAIFWTRNPRLTQVAEK
                     KIWVQIAKNFEPFIPRNEAEAQEMLLDFDEKIQFNASDLGDGKHKIGAEVFVSWLKHE
                     YTESFSEKTHSKEIEIEITK"
     gene            2858..3139
                     /locus_tag="VLB45_07375"
     CDS             2858..3139
                     /locus_tag="VLB45_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoL/Rpb11 RNA polymerase subunit family
                     protein"
                     /protein_id="HSD05550.1"
                     /translation="MDATITKSSPKEVYLTITKSDIGTLYIVQHELLKESSIEFAGVI
                     MKHPLTREYWMRVNTSKGSPLKEIEKATKTAIEYANELKKLVNSKIKGA"
     gene            3140..3454
                     /locus_tag="VLB45_07380"
     CDS             3140..3454
                     /locus_tag="VLB45_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription factor S"
                     /protein_id="HSD05551.1"
                     /translation="MKFCPKCEARLKQDSSDTNFTCPKCGHSEKGAKQRKQVTVESTS
                     TIDVLDENEKKETLPTIKIDCGKCGHNEAVWWMLQTRSADEPTTQFYRCTKCNYTWRN
                     YA"
     gene            3490..4236
                     /gene="pcn"
                     /locus_tag="VLB45_07385"
     CDS             3490..4236
                     /gene="pcn"
                     /locus_tag="VLB45_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030337 - DNA polymerase processivity
                     factor activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proliferating cell nuclear antigen (pcna)"
                     /protein_id="HSD05552.1"
                     /translation="MVFSAKTNGSDEWKAIISAISTLVEEATFESTGEGITFRGMDPS
                     HVALIDIAWPNSAFEKYECDSDVKFGVRIDEFSKLIKRADKKDSIEISISEDNLLIVT
                     IGKNKKYKMRLIESSATDTPLPKISYNAKIGVTSTEFDKILGDIQVVSEYLSINATAS
                     KAEFSGRGDSGEATITLEEGMPEMPDIEVKEDSTATYSLEYLNSIVKAVGTIAGTVTC
                     EYSTKMPLRLEFKVANVGRIHFYLAPRVES"
     gene            4271..5674
                     /locus_tag="VLB45_07390"
     CDS             4271..5674
                     /locus_tag="VLB45_07390"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HSD05553.1"
                     /translation="MKLVLKFGGTSISSVSNIRNVAKLIHSLSKEHKIISVFSAISGV
                     TDDLIRITALIEKGNKETANSLAKKIIKSHMQIANECVKNPKVRKELVEKMKTDLAEL
                     EELLHGMFLLGEVTPRSSDYLISFGERLSINLIAYALLDLGSKAVALTGKDVGIVTDS
                     NFGESKPLMDTTRLRVSHTIESLLAKKTLPVIGGFAGADQHGHITTFGRGGSDYTATI
                     VASSIKADEVWLMSDVDGLMTTDPKIVKNAKVLKEVSYTEAMEMALFGAKYIHPRALE
                     PLVTKKIPLRIRSTFNIKNSGTLVTASPKIDTQKTVKCVSAIRHTGLIDVRGGSMVGA
                     PGTAATIFSILAKAGVNVMMISQSPSESSISIIVKKNDLDKAVNTLEMNLLGKIIKKV
                     EVTVDVAIIALIGSGMRGIVGVASKVFAAVAKKGVNVVMIAQGSSELNLAFVVKDADC
                     NAAVQALHDEFELAKIN"
     gene            5735..7198
                     /locus_tag="VLB45_07395"
     CDS             5735..7198
                     /locus_tag="VLB45_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05554.1"
                     /translation="MNKYVVVIAIIAISATIFSIPLLTDQFVNAKTSTKKVQFTQTLT
                     SMQDPGQGHESHQLAIILSPNKGSLYDGSLTYAASEPVQIVILHEINKDDAKGQPTWT
                     VDGDTIFGLTLVDPGTSSGSYEFTGAAVALHTKNTNKFTATVSVDGWIRGQTPELSNQ
                     KTESLLERSVQISRANVPVEIPLHKGLFDGKPLYYIVTDANDKTHAKMITEKQKWKVE
                     IAPPLSKTPKDALDDVYMFLNGAEGNGMHGFQDEVFSSTPIQTEQYSAIRSVKHVTWK
                     DEKKAEILDSVQKILDAQKDGKIEIENSDVIINMPQIVWPDGQMTVKEDKTLTDETPY
                     GDAQILDINTDKMTVTFVAHRGWGPDGKTIYYIVTDATPSEAASMIGVTNAPKLAKTI
                     ASPAAVDLYQFTNGLKGSGPLGFQAGIASAALGDDNYSPLWRISVITWKEPLKATLLE
                     TISDVNSKKSSDDITVELARPMNSDHVVNCPFVDPFQ"
     gene            7247..8035
                     /gene="cobJ"
                     /locus_tag="VLB45_07400"
     CDS             7247..8035
                     /gene="cobJ"
                     /locus_tag="VLB45_07400"
                     /EC_number="2.1.1.131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014966021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030789 - precorrin-3B
                     C17-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-3B C(17)-methyltransferase"
                     /protein_id="HSD05555.1"
                     /translation="MDGKLFIVGVGPGHHDHMTFRAKQVIEESNTIVGYETYVDLVAD
                     MIEGKDVYRYAMTQEVERAHQCIELAKSGRIVSLVSSGDPGIYGMAGLIYEILAEAGW
                     DRESGLYVEVVPGVSSLNSCAALVGSPLMTDFAVLSMSDLLVPWEVIIKRVEAAAQGD
                     FVIVIYNPSSKKRIHQLQDTRKILLKYRAPNTPVAIVKGAYRESQTIVLTDLENLENH
                     SDKLGMISTVIVGNSSTYRYKNMMINPRGYTSKYNIVESTAKKT"
     gene            8037..9014
                     /locus_tag="VLB45_07405"
     CDS             8037..9014
                     /locus_tag="VLB45_07405"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03560 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HSD05556.1"
                     /translation="MKSIHVGLTLPQGWLDEFPDNNAYDQFLFSKSVALKAEQLGYDA
                     GYVYDHFIPYYGDKRTLPFFEAYTLLSAIAAITSKLRIGQVVTCNSYRSPALLAKMSS
                     TLDAVSNGRLEFGIGAGWFEYEYKSYGYNFDGPSVRIEQLDESLTIIKKMWQKEKSSF
                     KGKHYSIKNAICSPKPMQKPHPPIMVGGSGQKLIAVAAKHATRYNHPFGTPEILQSKM
                     EILKNQCTKIKRNFDEIENSVLLRVLVGNDKDEVKQIVSQLKKKNESITEFVTRSKDS
                     IALGTPDEVVEYLKQYVKIGIHYFIVNFIGLSNSLEMLSLFSKKVRPKL"
     gene            complement(9011..10063)
                     /locus_tag="VLB45_07410"
     CDS             complement(9011..10063)
                     /locus_tag="VLB45_07410"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HSD05557.1"
                     /translation="MDVTEKVALVAKDPTEEIVTNEELTNLFNTNSHPKHYIGLEISG
                     FLHLGSLILTGFKINDFIKAGVKCTVFLADWHTLINDKLDGNLETIKKVSEYYAKAFQ
                     FLCPGVEILQGSKLYESRKEYWKELVQFAKHVSLARTMRTLTIMGRSEGDEKLDLAKM
                     FYAPMQAVDIHSMDLDIVHAGMDQRKIHMLVREIFPKMKWKVPVAVHHHLLPGLSEPE
                     PASSEDSKIMSKMSKSKSGSGIFIHDSDEEIKSKIKKAWCPEGVVENNPLLQISRHLI
                     FHEFKELAIERPEKFGGNVSYTNYSQLESDFAQKKLHPTDLKKGVETCLIKIIKPIRE
                     HVVLQEEIIEAIKRST"
     gene            complement(10098..10661)
                     /locus_tag="VLB45_07415"
     CDS             complement(10098..10661)
                     /locus_tag="VLB45_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016940123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05558.1"
                     /translation="MKVHLHENQSGYKMFLYIFYICAVIMASITAFGIYAMLGEWSEK
                     GTYVMGEALVKTEFPYKHFAKLTTWLFFSSIIGWYCVSRIGWKKTVKIPSWRLSLLQL
                     MLVGFSVICLYEVMYNFTVLNAQIAAGIVDGKVPDIDSLTIAYPDSTRPWNLIFATKM
                     FLAGFIISSHALYLSTRPRKQEHELNA"
     gene            10753..11841
                     /locus_tag="VLB45_07420"
     CDS             10753..11841
                     /locus_tag="VLB45_07420"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010189627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HSD05559.1"
                     /translation="MKVPINIPIIGKEEIKEVQSVLSEGALTSAANSGGKRVQEFEKL
                     LSSFVKSRYAVAVNSGTAALQAALYALDVKKGDEVLLPSFTFVATANSIMSVGAKPVF
                     VDIKKENYTMDPNDLRKKITKKSKVIIPVHLYGNVAYIDEITEIANKHGLEIIEDAAQ
                     SLGSKYKGKQTGTFSKLGCFSMYAAKVMTTGEGGAIVTNNKKLFEKLRQIRNHGMQHG
                     YDTRILGLNLRLPEINAAIGKVQIKKLGKFLSQRRKNAKILTDLLSGLNVILPHERKG
                     EMVNWYLYTIATKNRDKIMKTLNSEGIGASVYYSPPVHKTPFYNKKQNLPVTDWAASS
                     VISLPVHPLVSKQNLYQTAKILRQTIRK"
     gene            11838..12323
                     /locus_tag="VLB45_07425"
     CDS             11838..12323
                     /locus_tag="VLB45_07425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007551609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydromethanopterin S-methyltransferase
                     subunit A"
                     /protein_id="HSD05560.1"
                     /translation="MNLIENIAGEICKKLFPINEEIFFGNLQSSLALCTLSSMQLLKE
                     ISSSTLMTKIAIAGRLLSENKGIDSLVKYVISNKNIKTIIICGKDTAGHKPGHSLLNL
                     YRNGIDHNGRIINSSSPDPILTLTKTEIMTFQTQVKLIDEIDQTDITKIRTLIDSVIS
                     V"
     gene            complement(12320..12652)
                     /locus_tag="VLB45_07430"
     CDS             complement(12320..12652)
                     /locus_tag="VLB45_07430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTPase"
                     /protein_id="HSD05561.1"
                     /translation="MDDRLESIFKMQKNLEKMMKLDRYPKDVEGKISALCTAIIHEAI
                     ELQRTTNWKWWKTPVVFDEAAAREELIDIWHFVVQASLELNMTPEDILNEYQKKNKIN
                     QERQKNGY"
     gene            complement(12688..13581)
                     /locus_tag="VLB45_07435"
     CDS             complement(12688..13581)
                     /locus_tag="VLB45_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012216127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HSD05562.1"
                     /translation="MLTVFGSVALDTIRTPTKVLKNVLGGAATFAAISASFFVKPGLI
                     AVVGQDFPKNYHNTLGRYLDLRGLERADGKTFRYDGSYDETLSHRTTNKTELNVLGSF
                     KPTVPEEYKKSEFVYLANNDPVQNTKIIKEFDKVKFSMCDTIDFWISTKRNDVIKMMG
                     VVDSVVINDEEAKLLTKTQNLIKCAKKIMEWGARYVIIKKGEHGSLLFYEDLVFPSPA
                     FSMENIVDPTGAGDSFAGGMIGYMSSKNNTKISTIREAAVYGNIMGSFAVEEYGLDGL
                     TRLRKSKIRKRFNQYKKMVRF"
     gene            complement(13594..14067)
                     /locus_tag="VLB45_07440"
     CDS             complement(13594..14067)
                     /locus_tag="VLB45_07440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05563.1"
                     /translation="MQKKSKVAVIAIAIAVIIGVFGYLQYASAMQLDVSIKDSEIVER
                     TSSGSLYQMQLEFENPSFMILNIGKTDFVISVEDEDLGAGVLEPFVIPAMGKIVVDAP
                     FLADNAVLDKYDNSEVSPSVKLSGITRYNLLFTTLDIPFTYYPTQEEAREFIHGQ"
     gene            14202..14663
                     /locus_tag="VLB45_07445"
     CDS             14202..14663
                     /locus_tag="VLB45_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010189620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05564.1"
                     /translation="MAKIRLKFGANEIEVESKDFYVDNQSIDEVVNNLSRYVSERTST
                     PEYVSSEQTQSTNYLSSLEDAEIHEPEFVRPISIDTTQIKSKINILSRDSFFEQPRTV
                     SEVVAQLYEYGWAARPLDVSKVLANMAFNGELQKDSRERRSYYTNKIMQLS"
     gene            complement(14875..15768)
                     /locus_tag="VLB45_07450"
     CDS             complement(14875..15768)
                     /locus_tag="VLB45_07450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05565.1"
                     /translation="MHKILTIVVMSGLLFSMSQVFGQDITFGEKPKQITEITIDVNGI
                     AHVVHKVEGSTKTQQIETYNGTMSNLSVTNENGGDVQYFTLEKQPIAVVLPPDKDMTF
                     IRYDLTDVLSLRNGVWTWDYTGQEITNFYFPGNVDIIWVNENPVYLAGKGIRQHGGAM
                     KLEYIADEPMILKETTWENEKFTVGIRTLTSIDMFEFDQSSKSLSFDISDGGSYTTAI
                     IPLKLLWEPYDVYLNSNGTENSEFYNNGTHVWIGFKPETSGNVQIIGTTVIPEFPLFV
                     PLVIGISAVILIQFRNRFHFH"
     gene            complement(15778..16257)
                     /locus_tag="VLB45_07455"
     CDS             complement(15778..16257)
                     /locus_tag="VLB45_07455"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HSD05566.1"
                     /translation="MTKATIETNFGKIAFDLLPNLAPETVRNFVQLAKSSFYDGTLFH
                     RVIPGFMIQGGDPNTKKPDKSKWGMGGPGHTIKAEFSSKSHLRGIVSMARAMDPNSAG
                     SQFFIVTTDSTFLDRQYTVFGQVTEGMDVADKIVNLPRDKNDCPHQEAKMLHVTISE"
     gene            16530..16775
                     /locus_tag="VLB45_07460"
     CDS             16530..16775
                     /locus_tag="VLB45_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05567.1"
                     /translation="MNRSTQSTNIKQAINALLDQGVTDKQDIYTKVVEELGVPRPTVR
                     RVARDLRNELLQKIQILQSEVPRPGYFGNKDTESLTA"
     gene            16859..17329
                     /locus_tag="VLB45_07465"
     CDS             16859..17329
                     /locus_tag="VLB45_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014966005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05568.1"
                     /translation="MGYLGNHLATVVTSIFAATLTGLYFVLPPATHFILIIAIIATWF
                     LAFICWIAQKSNDYMHKHGSHEVKKKLTENTSSHVQSAQISSEELNKARVELSTDLDA
                     LRDKVMIKDKEIENLKKEISNLKTLVEIESIRTELANLKMLASQENSKRKTKKK"
     gene            17507..17932
                     /locus_tag="VLB45_07470"
     CDS             17507..17932
                     /locus_tag="VLB45_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010189605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05569.1"
                     /translation="MKSVMGLGFAAFAVLFVMLPSPTSSATDEFPLEPQLVADNNSVI
                     LEKKIISMHISEDNQLPWGFVEGNVINPAEGYPVIIQIYKNGEPVHFAQTDVQEDGSY
                     EYRFRVRNVDGDQVINIFQGDYTVKIFKTVYATPQAQIV"
     gene            complement(17929..18444)
                     /locus_tag="VLB45_07475"
     CDS             complement(17929..18444)
                     /locus_tag="VLB45_07475"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HSD05570.1"
                     /translation="MKAVVISGSPRKGANTKIMMEYVYNYAKTKNPDTKFIDLSEGMV
                     DCYRGPGEEYSQTTIQAAKDVTEADVWFVGSPVYNSLFSSALKNLFEYINYKETAGKT
                     AGVAILGSGNISFTNVQNVITGLMTYFRVITNPKAVHMTADQIKDGKIVDENAMVRLR
                     EFVDETLKLAS"
     gene            complement(18756..18848)
                     /locus_tag="VLB45_07480"
     tRNA            complement(join(18756..18815,18834..18848))
                     /locus_tag="VLB45_07480"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18793..18795),aa:Arg,seq:cct)
     gene            complement(18874..19584)
                     /gene="bluB"
                     /locus_tag="VLB45_07485"
     CDS             complement(18874..19584)
                     /gene="bluB"
                     /locus_tag="VLB45_07485"
                     /EC_number="1.13.11.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016722 - oxidoreductase activity, acting
                     on metal ions [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0032363 - FMN catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5,6-dimethylbenzimidazole synthase"
                     /protein_id="HSD05571.1"
                     /translation="MNDDFSQDEKNGFYKAVFSRRDVRSHFTSDPIDDKTLSKILNAA
                     HHAPSVGFSQPWNFILIKDQNTRKKIKESFEVERKRSSALIEDPKRSKYLSLRLEGIL
                     DSAVNICVTYDPSKFGPFVIGRSSIPETGIYSVCCAIQNLWLAARTEDIGLGWVSILS
                     NDDLKKTLDIPEHVVPIAYLCLGHVSKFAEKPDLETVGWLPRLDLKDVVFYEKWNKVE
                     DPSWNQIHQMIISNLDYA"
     gene            19671..19744
                     /locus_tag="VLB45_07490"
     tRNA            19671..19744
                     /locus_tag="VLB45_07490"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19705..19707,aa:Val,seq:gac)
     gene            19778..20176
                     /locus_tag="VLB45_07495"
     CDS             19778..20176
                     /locus_tag="VLB45_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HSD05572.1"
                     /translation="MVKFTQKEIDFLQNNEACRIATSHNDIPHVVPVTFYFENGNFYI
                     AVDYDTKKYSNLKKNNRAAITVDIYAAGKHKAVIVQGATEFIEKGSEFKRLYETFHKK
                     FSWVRENPWKEGEAPFVKLMPKTKTSWGLN"
     gene            complement(20173..20670)
                     /locus_tag="VLB45_07500"
     CDS             complement(20173..20670)
                     /locus_tag="VLB45_07500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05573.1"
                     /translation="MVSRGRISIIGAFIGLGLIMLGFVYYASLDNPQLEQVQVDLVSV
                     KVLDTNSIDKRADLEVVFLVTNPSQRTLTLSNISYVLFANDRPVGNGEYSTEDVAMTG
                     RAAFYPGQSIEIPSTLNLVYTDQVADVYSAIINGEHVSYKITGQVSVESAWSIVEKQF
                     ESTLN"
     gene            20756..21265
                     /locus_tag="VLB45_07505"
     CDS             20756..21265
                     /locus_tag="VLB45_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010189599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HSD05574.1"
                     /translation="MIDWTMPIADALITWAHLISASIWVGGSIFIGIVLAPMLKTISP
                     SVEERLKIMIRAGRKFNKIAVPSLAILIATGLYNSYNILNRPDMLFSTNYGIILTIKI
                     VLVISLIAAFAAHTRIIRKEIEKKIETKEMSEDSIQKLRSKIILLGKIIVFISVAILL
                     MAAMLDAGI"
     gene            complement(21248..22276)
                     /gene="rtcA"
                     /locus_tag="VLB45_07510"
     CDS             complement(21248..22276)
                     /gene="rtcA"
                     /locus_tag="VLB45_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007551591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003963 - RNA-3'-phosphate cyclase
                     activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HSD05575.1"
                     /translation="MKPIEIDGAFGEGGGQIIRSAVTLSCLTQKPVEITNIRKNRKVP
                     GLRAQHMTAIKILAKICNAKVEGLHVGSTAIKFIPSNVIDTNLKEDVGTAGSIPLILQ
                     VLIPTVAISKKKLKMTITGGTDVLWSPTSDYTRFVLREAYSRMGINFSIDIKKRGYYP
                     RGGGMVDVDVFPCEKLKPISLLKRTSKSVKITCSHSMISSQMMEHEITKAKEILEKSG
                     FDCQYEIKNELAYDIGCSFLAYAHDESSVNGSDSIYNKGVDGIGQSIAKRFLESEFGV
                     DQFLSDMLVLPMSLADGTSVFRVDKITKHLETNLFVTSEILNCKYGIGKFDNGFEVRI
                     TGNSNSSI"
     gene            22343..22840
                     /locus_tag="VLB45_07515"
     CDS             22343..22840
                     /locus_tag="VLB45_07515"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007551590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide adenylyltransferase"
                     /protein_id="HSD05576.1"
                     /translation="MNGLFIGRFQPFHLGHLDAIKFGLSKIENLWIGIGSSDKSNEKR
                     NPFSADERKQMILSSLDDDFLKRIKIYYISDVDDHEKWTYHVDSLVPKYDVVFSNDDF
                     THTLYQKRGIKVISVPLKERENLSGTNIREMIAMGKDWMALVPEGTKNVLLKMDAERR
                     LSKIL"
     gene            22883..24025
                     /locus_tag="VLB45_07520"
     CDS             22883..24025
                     /locus_tag="VLB45_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HSD05577.1"
                     /translation="MEYLVMLPGPTNVPERVTRAMFTHMINHRSKDFVKLYTDVVEKS
                     QKVFETSGDVIALSASGTGAVEASVVNLIKKGDKVIIPVNGEFSNRLATLLEWQGANV
                     IRLTTPFGENASFDQVKEAFDNNKDVKAFYVVWNETSTGTMVKYLDKISKLTSRNDSY
                     YVVDGVSIVGGEELRMDKWGIDVCVTGAQKALAAPPGISPIAISPKAKKYMIANPPPT
                     FYFNLARYFKYYDDTKETPFTPALPLLYAYREALDIILEEGMQARINRHRVCSNAFYS
                     GLSAMGLTPFAKEDARSTVVIALNYLQGLEDKTFRSTLSEKFRVLVAGGFGDLKGKVF
                     RVGCMGEVHKYHVMRTISAISSTLDMMGHKTNPEALKVAEEKLKAL"
     gene            24069..24821
                     /locus_tag="VLB45_07525"
     CDS             24069..24821
                     /locus_tag="VLB45_07525"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HSD05578.1"
                     /translation="MTFQKGTLVLVDYTAKVKDTGEVFDTTREADAKSSSLYDTNARY
                     QPRLVSVGESWVLKGLDDALLDTKVGDKLTVEVPPEKGFGTRDPSKVRMIPLRKLGED
                     AEKVSVGDAIDVDERTGIVRFIGSGRVQVDFNHRFAGKTILYDVNIVKSLDTDNDKIL
                     GLLKRRLPVDDSKLQFKINGKEIDITMPEETFLAEGLQVIKRAIVNDIFKFVPALDKV
                     NFIEGYKKAEKTEAKQVESKPAEAKAAPPKAA"
     gene            complement(24823..25881)
                     /locus_tag="VLB45_07530"
     CDS             complement(24823..25881)
                     /locus_tag="VLB45_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012216111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-1-phosphate dehydrogenase"
                     /protein_id="HSD05579.1"
                     /translation="MKFHQKPMSSHVMELPRQIVIGEKNISDVGKFIADLANPRKVSL
                     VSGEQVRKIVQTKIVKSLLKSKIKNVWHISTSNDVKTAKKTQQSISKDKSDLIIGIGG
                     GRSVDIAKMIAFNLKKPFVSIPTSASHDGIASPFVSLRGDKPHSIVATAPLGVFVDIE
                     VLKKAPKKLLASGCGDLMAKITAVRDWELARDDVGEYFGTYAANLASMSAKIVIENSK
                     SGKKPDVRMIVEALISSGVAACIAGSSRPCSGAEHLFSHALDYLVPGVGLHGEKCGIG
                     AILISKLQGQDWKRIVSTLESVGAPTTAKQIGLKPDMLAKALVMAPSLRKERYTILNK
                     IKMTREKAISLAKSTGVL"
     gene            25970..26602
                     /locus_tag="VLB45_07535"
     CDS             25970..26602
                     /locus_tag="VLB45_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome subunit beta"
                     /protein_id="HSD05580.1"
                     /translation="MSDYVEQHTLHGTTTVGISCTDGVVLCADMRASAGYFIANNNTM
                     KIQKIDYHAGMTIAGGVADAQNVTDMLRYHSNIHRIEKQEYLPIKSIARLASLIFFQN
                     RYYPFVADILVGGYDKHGPSLYNIDMFGSLDKKTFVTTGSGSPVAYGLLENEYRDGLT
                     VEQGKIIALRAVKAAITRNIGTGDGINVAIIDQKGYRLLTKEQKRAIITL"
     gene            26610..28541
                     /locus_tag="VLB45_07540"
     CDS             26610..28541
                     /locus_tag="VLB45_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-CASP ribonuclease aCPSF1"
                     /protein_id="HSD05581.1"
                     /translation="MQRKQQQRDLTPATNIMGTILQSIPKEADVTKIDYEGPRIAIYT
                     KKPRYLMEHNEIVSNMVNVIKKRIVVRTEESIRKTEEEARQILTQVLPKEADLRGTFF
                     DTAIGEVTVEVKKPWLLFNNEAFNMADLVEKSGWRFRIRKATKIQSQTIQTINYNLKI
                     SSSERSKHLKQVGEQIFRPKLSETAEVSLMTLGGFSQVGRSCMLLTTHESKILIDCGV
                     NPGAKSAMEAYPRLDWANITLDELDGVVISHAHLDHTGFLPALFKYGYKGPVYCTEPT
                     LPMMNLIQLDAIKVAAAQGKIPLYSERDVKQVMKQTITLPYGTVTDISPDIKLVFSNA
                     GHILGSATCHFHIGNGDHNFVYSGDLKFGKTMLLESAVWNYPRVETLLIESTYGAKED
                     IQPTREEVEGAFIRSVNETLRNGGKVLIPIPAVGRAQELMMVIDMYMKAGQIVESPVF
                     MEGMISEATSIHEAYPEYLARELRQKILETDDNPFDSEYFTNIEHADAREEPLREGPC
                     IIIATSGMLEGGPVLEYFKNIAPSQKNKILFVSYQVNGTMGRRVLDGARQVSVIGKDG
                     KVEIININCSTEKLDGFSGHSDYNQLMSFVHKLRPKLRRVIVNHGERRKSENLSMSIH
                     RMFRIMTTCPQVQEAIKLF"
     gene            complement(28533..28811)
                     /gene="sepF"
                     /locus_tag="VLB45_07545"
     CDS             complement(28533..28811)
                     /gene="sepF"
                     /locus_tag="VLB45_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HSD05582.1"
                     /translation="MQAQKAPTYLKAITLRDTSDVHAVKEDVKKNMILVLRVTPLAQK
                     NVEELRKVVEEIYAIAKSIGADIARLGEERIIVTPPGVKIWRAEYDLK"
     gene            complement(28884..>29412)
                     /locus_tag="VLB45_07550"
     CDS             complement(28884..>29412)
                     /locus_tag="VLB45_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA
                     (5-methylaminomethyl-2-thiouridylate)-methyltransferase"
                     /protein_id="HSD05583.1"
                     /translation="ELNGLIKREDLGKIRGRSRKDQLKMAQEFGIENPPNAGGGCLLT
                     DPAFAIRAKDLFNHIQTPTTNDIDLLKIGRHFRFDEKTKLIVGRNQDENEVLKALALD
                     GDILLEAKDHVGPVTLIRGENASSHVEFGATITLRYGDAPKDTPSVVTVQKNGTSSEI
                     SAIPASESAYLQYRI"
BASE COUNT         9677 a         5501 c         5270 g         8964 t
ORIGIN      
        1 tagattctac ttttaaatgg aaaatcttgt gcgacatcat aatctgaatt cattattata
       61 ttacaacatc actacatgtt ttgttgctta aaaatcatga taatgatgta accaaaaatg
      121 ttgttttgga tttagatgac atactttcac aatccaaaat taattctgtt ttatactctg
      181 atccatttct gaaagcaggt tttcttgcaa aacttgttca ggatacaaag atcaatgtac
      241 tctatcttga tcttgatctg ttgtacagtg gttatcttgc atctgaaata cttggtaccc
      301 cacaaaacct cgttttattt cggccaactc ttgaactatg gaatcagatg ctaactgacg
      361 tgttagtaaa attaagtaat aaaaaatctc tagttgtaat tgattcactt aacggcctgt
      421 acaatatttt aaatgaaaga aaagaagttg gcaagcttgt tacttcatat gtaatgttaa
      481 tcaccagcat ggcaaaaata actaattctt acatcgttgt ggcaagtatg gtcagattta
      541 aaaaagaaga aggttgggta ctatctccta caggaaagcg ccttattgag acaagtatgg
      601 caaaaaaaat acttttggac caaaatgatt cagaaataac gctggatttt cttaaggaga
      661 agaaaaaaat caccattcca ttaaatgcaa ttccgttagt ctaatcgttt tattatatgg
      721 tatccaaact gcgtctttac tatgtctgaa acttgtcctt tctctaatga aaatgccgcc
      781 ttttcaaatt ctggcaccat cattcctcta ccaaaaaaac caaggtctcc acctcttctt
      841 tttgatccat cgatggaaaa ctgcttagca agattcgcaa agctttcacc ttttacaatt
      901 tttgttttta gttcttgtgc taaacttagc ttttctacta gtatgtgtgc acaatgaact
      961 ttgtttccca ttacaactaa aaataaaatc aaattataaa aaaacgtcga tcaactactt
     1021 ttacaaaata tttgtcaaac cattccagat tcttagaatg atctaagtgt aatcaactga
     1081 atcatcacac cactttaaca ccgttataaa atttcaaacg ccgttataac ttgggttttt
     1141 tgagcaagtt atattaagat ccgtcaagaa caatgatcga taaacatgcc agttggaatt
     1201 attccagata taggcgaaca aatgtgtata ggttgtgcac tctgcgtaga gatttgtaca
     1261 tccctaggac cagacgtatt aagagtaaaa ccagtagaag gctggaaacg aggaaaagca
     1321 tttgtctttt atccagaaag atgcatctct gatggagcat gcattggtgt ttgcccaaca
     1381 aaggcaatct tttggatgag accaatggac tttaccgtcg gacaaccagt tccattatac
     1441 aggaactccg tattcgtcaa aggttggact gaactagcag aatagatagt ccgacaataa
     1501 ctccttttta ttatttataa tcagtgtgaa ctttactcag accatcttcg ctgtctaaat
     1561 atatggaaaa attcaacaat tatcattgag caaacctgga aatctatgga gaaaagtaca
     1621 cggtaggtta acaaaaaaac caaccaactt tagttggctg attaacaaca aacttgcagg
     1681 cagtggtatg cccacatctc ttgcagaact agattgggtt ttcaaacaag gcgtcaaatc
     1741 aataataaca atgactgaag aatctcttcc tgaatcttgg attaaagatg tacgatatct
     1801 tcatgtacct accgaagatc tgtctgcacc agatattgaa aaaatagatc ttgctgttga
     1861 ctttatacaa gaaagaataa aaaataatga agctattatg gttcattgtg ctgcaggaat
     1921 tggtagaact ggcaccattc tagcttgtta cctcataaaa tatcaaaatt tgtctgtaaa
     1981 aactgcaatt gataaagtac gccaagaaag accaggctct attcagtcag agtcccaaga
     2041 aatagcgata ggtctttatc acaaatttat caacaagtaa ctattctggt attgaactag
     2101 ttaccatctt gccgttttct agctttatga acaaatacct gttgtccttg aagattagcg
     2161 ttatttcgtt ttctttttct gacttgtctt caacttcaag tagttcttgt cctctaattc
     2221 catcaaattt tgccatacgt gatttatttt aaaaactcta tttataacct atttggtaat
     2281 ctcgatctca atttcttttg aatgtgtttt ttcactaaaa gattcggtat attcatgttt
     2341 taaccaagaa acaaaaactt ctgcgccaat cttgtgtttg ccatcaccta gatctgatgc
     2401 attaaactga attttttcat caaaatcaag aagcatttct tgggcttcgg cctcgtttct
     2461 tggaataaat ggttcaaaat tctttgctat ctgcacccag atcttttttt ctgctacctg
     2521 agttagtctt ggattacgcg tccaaaaaat agcagctttc ctgtatgtat caaccggctt
     2581 tccaagctca tgctttcgta atcctccact gaagagcttt attccatact ttatcttgaa
     2641 agcattgtca tgtttactgt atgctttttc ccaatttttt tcattccatg attcacgtaa
     2701 tgctcccaaa acagaaaatt tcacatttat cacaacattg tcgtctttct tgaatttgtc
     2761 atttggtttt accaactcaa cattgccgat aaagctcttt tcactagtca actcatcgta
     2821 atgatttata tcctcaataa gtattttttt gatttacatg gatgctacca taacaaaatc
     2881 ttctccaaaa gaagtttatt tgactataac caagtccgac attggtacat tgtacatcgt
     2941 acaacatgaa cttctaaaag aatctagcat agaatttgct ggtgtaataa tgaaacatcc
     3001 tcttactaga gaatattgga tgcgtgtaaa tacatcgaaa ggaagtccac ttaaggaaat
     3061 tgaaaaagca acaaaaaccg caattgaata cgcaaacgaa ctaaaaaaat tagtaaattc
     3121 aaaaatcaaa ggtgcataaa tgaaattttg ccccaagtgt gaggcacgtc taaaacagga
     3181 cagctctgat accaacttta cttgtcccaa atgtggtcac agtgaaaaag gtgcaaaaca
     3241 aagaaaacaa gttacagtag aaagtacttc gaccatcgat gttttggatg agaacgagaa
     3301 aaaagaaaca cttccaacca taaaaattga ttgtggaaaa tgtggacata atgaggctgt
     3361 ttggtggatg ttacaaacaa gaagtgcgga tgaaccaaca acacagtttt atcgttgcac
     3421 caaatgtaat tacacttggc gcaattacgc ataacgttta acttgaacat attagacatg
     3481 tgattcagtt tggtattttc agctaaaact aatggctctg acgaatggaa agcgattatt
     3541 tctgcaattt ctacactcgt tgaagaagca acttttgaat caacaggaga aggaataaca
     3601 tttcgtggaa tggatccatc acatgtagca ttaatagaca tagcatggcc aaactccgca
     3661 tttgaaaaat acgagtgtga tagcgatgtc aagtttggtg ttagaataga tgaattttcc
     3721 aaactaatca agagggctga taaaaaggac agtatagaga ttagtatctc agaagacaat
     3781 ttattaattg taacaatagg aaaaaataaa aaatacaaaa tgaggctaat tgagagctct
     3841 gcaacagaca cgcccttgcc aaaaatttcc tataatgcaa agattggtgt tacatctaca
     3901 gagtttgata aaattctagg tgacattcag gtagtatcag aatatttatc cataaacgca
     3961 actgcatcca aagcagagtt ttctggaaga ggagattcgg gggaagctac gatcactttg
     4021 gaagaaggaa tgcctgaaat gccagatatt gaagtaaagg aagacagtac agcaacttat
     4081 agtttggaat atcttaacag cattgtaaaa gctgttggta ctatagcagg tacagtaact
     4141 tgtgaatatt caactaaaat gccacttagg ctagaattta aagttgctaa cgtaggcaga
     4201 attcatttct atttggcacc cagagtagaa agttaaagca tttaaggaca atcaagtcag
     4261 gtatactaaa ttgaaactag tattgaaatt tggtggcaca tcgatttctt ctgtttctaa
     4321 cattaggaat gtagccaaac taatccattc attatcaaaa gagcataaga taatttctgt
     4381 cttttctgca ataagtggag taaccgatga tctgatacgc attactgctc tgatagaaaa
     4441 aggaaataaa gaaactgcaa atagtctagc aaaaaaaata atcaaaagtc atatgcagat
     4501 agcaaatgaa tgcgtaaaaa atccaaaggt aagaaaagag ctagtagaaa aaatgaaaac
     4561 cgatctagcg gaattagaag aactgttaca tggaatgttc ttgcttggtg aggtgactcc
     4621 tcgctcttct gattatctaa tatcgtttgg ggagagactg tctatcaatc tgattgcata
     4681 tgcattgctt gatttaggtt caaaagctgt agctcttaca ggcaaagacg taggcatagt
     4741 gacagattct aactttggag aatcaaaacc gcttatggat acaacaagac ttcgtgtatc
     4801 tcacacaata gaatctctct tagcaaaaaa gacgctgcct gtaattggtg gctttgctgg
     4861 agccgatcaa catggacaca taacaacatt tggaaggggg ggctctgatt acaccgcaac
     4921 aattgtggca tctagcataa aggctgatga agtttggtta atgagtgatg tagatgggtt
     4981 aatgacgact gatccaaaaa ttgttaaaaa cgctaaggtg ctcaaagaag tttcttatac
     5041 agaagctatg gagatggcac tttttggtgc aaaatacatt caccctcgtg cacttgaacc
     5101 attagttaca aaaaaaattc cactgagaat aagaagtaca tttaacatta aaaattctgg
     5161 aacccttgta accgcatctc caaaaattga tacacaaaaa actgtcaaat gtgttagtgc
     5221 catacgacat actggactga ttgatgttag aggaggtagt atggtaggtg cacctggaac
     5281 agcagcaact atattttcta ttcttgcaaa agccggagtc aatgttatga tgatctcaca
     5341 gagtccttcc gaatcaagca tctccatcat tgtaaaaaag aatgatcttg acaaggcagt
     5401 aaatacactt gaaatgaatc tgcttggtaa gataatcaaa aaagttgagg ttacagttga
     5461 tgttgcaatc atcgcattaa ttggttctgg aatgcgagga attgttggcg tggcttccaa
     5521 agtctttgca gcagtagcaa aaaaaggagt taacgtagtt atgattgcac aaggttcatc
     5581 agaacttaat cttgcgtttg ttgtaaaaga tgctgattgt aacgctgctg tacaagcatt
     5641 acacgacgaa tttgaacttg caaaaataaa ctaatttgct atatctgcac aaaacataga
     5701 aaaagcattt atgtaaatcc gcgctctcga ctctatgaac aaatatgtag ttgtaatagc
     5761 aattattgca atttcagcta caatattctc tataccacta ctgactgatc agtttgtaaa
     5821 tgcaaagacc tcaacaaaaa aggttcaatt tacacaaaca ttaacttcaa tgcaagaccc
     5881 tggccaaggt catgaaagtc atcagttagc tatcattcta tctccaaaca agggttcatt
     5941 gtatgatggt tcactgacat atgcagcaag cgaaccagtc caaattgtta ttcttcatga
     6001 aattaacaaa gatgatgcaa aaggacaacc gacatggaca gtagatgggg acacaatctt
     6061 tggtttgaca ctagtggatc ccggtacaag ttctggttct tacgaattta caggtgctgc
     6121 agttgcctta catacaaaaa acacaaacaa gtttacggca acagtaagtg ttgatggctg
     6181 gatacgagga caaacacctg aactatcaaa ccaaaaaact gaaagtttac tggaacgatc
     6241 tgtacaaatc tcaagagcaa atgttcctgt ggaaattcct ctacacaaag gactctttga
     6301 tggaaaacca ctttactaca ttgtaactga cgcaaatgac aagacgcatg ctaaaatgat
     6361 cacagaaaaa caaaaatgga aagttgagat agcgccaccc ttgagtaaaa ctccaaaaga
     6421 tgcattagat gatgtctaca tgtttcttaa tggagctgag ggaaatggaa tgcatggatt
     6481 tcaagatgaa gtcttttcaa gcacgccaat acagacagag cagtatagtg caatacgttc
     6541 tgtaaaacat gtaacttgga aggatgagaa gaaagcagaa attttagatt ctgtacaaaa
     6601 aattcttgat gcacaaaaag atggaaaaat agaaattgaa aatagcgatg ttatcataaa
     6661 catgccacaa attgtttggc ctgatggtca aatgactgta aaagaagaca aaaccctgac
     6721 tgatgagaca ccatacggtg atgcgcaaat tcttgatata aacaccgaca aaatgacagt
     6781 aacatttgta gcacataggg gatgggggcc agatggcaag acaatttact acattgttac
     6841 tgacgcaact ccctcagaag cagctagtat gataggagtt acaaatgcgc ctaaacttgc
     6901 aaaaacaata gcgagtcctg cagctgtgga tctatatcaa tttacaaacg gactgaaagg
     6961 ctcaggacca cttggatttc aagctggaat tgcttcggct gcactaggtg atgataatta
     7021 cagtccatta tggaggatat ctgtgattac ttggaaagaa ccactaaaag ctacacttct
     7081 tgaaacaata tctgatgtta actcaaaaaa atcatctgat gatataacag tagaacttgc
     7141 acgaccaatg aatagtgatc atgtagtaaa ctgtccattt gtagatcctt tccaatagaa
     7201 aaatttcgtg aaaacggata aattgcttta aaaaagaaaa tcagtcatgg atggaaaatt
     7261 gttcattgtt ggtgtcggtc ctgggcatca tgatcatatg acttttcgtg caaaacaagt
     7321 aatcgaagaa agtaatacga ttgtaggata cgaaacctat gttgatctag tagctgatat
     7381 gattgaaggt aaagatgttt acagatatgc tatgactcaa gaggtagagc gagcgcatca
     7441 atgcatagaa ctcgctaaat cgggaagaat agtctctctt gtttcaagtg gagatccagg
     7501 aatttatggt atggcaggac tgatatatga aatactggca gaagccggct gggataggga
     7561 aagtggatta tacgttgagg tagttcctgg tgtgtcatcg cttaattcct gtgccgcatt
     7621 agtgggttct ccattgatga ctgattttgc ggtactgagc atgagtgatt tgcttgttcc
     7681 ttgggaagta ataataaaaa gagtagaagc tgctgcacaa ggtgattttg ttatagtcat
     7741 ttacaatcct tccagcaaaa agagaattca tcaattacaa gatactagaa aaattctact
     7801 aaaatacaga gcacctaaca caccagttgc aatagtaaaa ggggcataca gagaatctca
     7861 gacaatagtt ctaactgatc ttgagaatct cgaaaatcat tctgacaagc ttggtatgat
     7921 aagtacagtg attgtaggaa attcatctac ttatagatac aaaaacatga tgattaatcc
     7981 acgtggatac acttcaaaat acaatatcgt agaatctact gcgaaaaaaa cttaacatga
     8041 aatcaatcca tgtgggttta actttaccgc aaggatggct agatgagttt cctgataaca
     8101 atgcgtatga ccaattcctt ttctccaaat ctgttgcgct aaaagccgaa caacttggat
     8161 atgatgcagg atacgtatat gatcacttta ttccgtatta tggagataaa cgaacacttc
     8221 ctttctttga agcatacaca cttctgtctg caatagcagc tattacatct aaactgagaa
     8281 ttggtcaagt cgttacttgt aattcatatc gttcgcctgc acttttggca aaaatgtctt
     8341 ctactctaga cgctgtgagt aacggaagat tggagtttgg cataggtgct ggctggtttg
     8401 aatatgagta caaatcatat gggtataatt ttgacggtcc atcagtaaga attgaacaac
     8461 ttgatgaatc attaacaata atcaaaaaaa tgtggcaaaa agaaaaatct agttttaagg
     8521 gaaaacacta ttctattaaa aacgccattt gtagtccaaa accaatgcaa aaaccgcatc
     8581 ctcctatcat ggttggtggt tcaggtcaga aactaatcgc agttgctgca aaacatgcca
     8641 cacgatataa tcatccattt ggtacaccag aaattttaca atctaaaatg gaaatactaa
     8701 aaaatcaatg cacaaaaatc aaacgaaatt ttgatgaaat tgaaaattca gtcttgttgc
     8761 gagtactagt tggaaatgac aaggatgaag taaaacaaat tgtatcacaa ctaaaaaaga
     8821 aaaatgaatc aataactgaa tttgtcacgc gttcaaaaga tagtatagct cttggtacac
     8881 cagacgaagt agtggaatat ctaaaacaat atgtgaaaat tggtatacat tattttattg
     8941 taaactttat tggtttgtca aactcgcttg aaatgctttc attattttca aaaaaagtaa
     9001 gaccaaaact ctaggtactt ctttttattg cctcaataat ctcttcttgc aggactacat
     9061 gctctcgtat tggtttgata atctttatga gacaagtttc tacacctttt tttagatcag
     9121 tgggatgaag ctttttttgt gcaaaatctg attctagttg agaataattg gtatatgata
     9181 catttccacc aaatttttca ggtctttcta tagcgagttc tttgaattca tgaaaaatca
     9241 agtgccgtga aatttgtaac aatggattgt tttctacaac accttctgga caccaggcct
     9301 tcttaatctt agattttatc tcttcatcag aatcatgaat gaatattcct gagccactct
     9361 tggatttact catcttactc ataattttag aatcttcact agaagcaggt tctggttctg
     9421 ataggcctgg cagaagatga tgatgaacag ctactggcac cttccatttc atctttggaa
     9481 aaatttctct tactagcatg tggatctttc tttgatccat tcctgcgtga actatgtcta
     9541 gatccattga atgaatgtct actgcctgca tgggggcgta aaacatcttg gccagatcaa
     9601 gcttttcatc gccttctgac ctgcccatga tggttagtgt cctcattgta cgtgcaagcg
     9661 acacatgttt tgcaaattgt actaactctt tccaatattc ttttcttgat tcgtaaagtt
     9721 ttgagccttg aagtatttcg actccaggac aaagaaactg aaaggctttt gcataatatt
     9781 ctgatacttt ttttatggtt tctaaattac cgtcaagctt atcgttgata agagtatgcc
     9841 aatcagctag aaaaactgta cacttgactc cagctttgat aaaatcattt attttaaaac
     9901 cagtaagtat gagactacca agatgtaaaa aaccagaaat ttctaacccg atgtagtgct
     9961 tgggatggga attggtattg aaaagatttg tcaactcttc atttgtgaca atttcttctg
    10021 taggatcttt tgcaacaagt gcgacttttt ctgtaacatc caatttgaat cagatttgct
    10081 tttgtaaacc tataaagtta cgcattaagt tcgtgttctt gttttcttgg tcttgtactc
    10141 aaataaagag catgtgagct tatgataaat ccagcaagaa acatttttgt ggcaaatatc
    10201 aaattccaag gtcttgttga atcaggataa gcaattgtta atgaatcgat atctggaact
    10261 ttgccatcta ctattccagc agctatttgc gcattaagaa ctgtaaaatt atacatcact
    10321 tcataaagac aaattacaga aaatcctacc agcatgagct gcaaaagtga taatctccat
    10381 gatggaatct taacagtttt tttccaacca attcttgata cgcaatacca tcctattatt
    10441 gatgagaaaa ataaccaagt tgttaatttg gcaaagtgct tgtacggaaa ttcagtcttg
    10501 acaagtgcct ctcccatgac ataggttcct ttttctgacc attctccaag cattgcgtat
    10561 attccaaatg cggttattga tgccattatt actgcgcaaa tatagaaaat atacaaaaac
    10621 atcttgtagc ctgactgatt ctcgtgcaaa tggactttca tttaactccg ttccatttgt
    10681 tccaaaatat aaaaattggt tttggcttga ttggtgaggt ttattatctc agtaataatc
    10741 acaaattaat gattgaaagt tccaataaat attcctatta ttggcaaaga agaaatcaaa
    10801 gaggtacaat ctgtactttc agaaggagct ctgacttctg cagcaaattc tggcggaaaa
    10861 cgcgtacaag aatttgaaaa actcttatca tcatttgtta aatcaagata cgcagttgcg
    10921 gtaaattctg gcactgccgc attacaagca gcactttatg cacttgatgt caaaaaagga
    10981 gatgaggtac ttcttccttc gtttactttt gttgcaacag caaattccat aatgtcagtt
    11041 ggtgcaaaac cagtttttgt tgatatcaaa aaagagaact atacgatgga tccaaatgat
    11101 ctcaggaaaa aaatcacgaa aaaatctaaa gtgataattc cggttcatct gtatggtaat
    11161 gtagcgtata ttgatgaaat aactgaaatt gcaaataaac atggtttaga aataattgaa
    11221 gatgctgcac aatctcttgg ttcaaaatac aaaggaaaac agacaggaac tttttccaaa
    11281 cttggttgtt ttagtatgta tgcagcaaag gtaatgacaa ctggggaggg aggagcaatt
    11341 gttacaaata acaagaaact atttgaaaaa ctcaggcaaa taagaaatca tggaatgcaa
    11401 cacggatatg acactagaat tcttggtcta aacctaaggc tgccagagat aaatgctgca
    11461 attggaaagg ttcagattaa aaaactgggt aaattcctta gtcaaagaag aaagaacgca
    11521 aaaattctca ctgatctctt gtctggtctg aatgttattc ttcctcatga aagaaaaggc
    11581 gagatggtta attggtatct gtatacaata gctactaaaa atagagacaa aataatgaaa
    11641 acgttgaact ctgagggaat aggagcatca gtttactact ctccacccgt acacaaaact
    11701 ccattttata ataaaaaaca aaatcttcca gtcacagact gggcagcatc atctgttatc
    11761 agtttgccag tgcatccgct tgtaagcaaa caaaacttgt atcaaactgc aaaaattcta
    11821 cgtcaaacaa ttaggaaatg aatctaatag aaaacatagc cggagaaatt tgcaaaaaac
    11881 tatttccaat taatgaagaa atattttttg gaaatctaca atcttcactt gcattatgca
    11941 ctttatctag catgcaattg ttaaaagaaa tttcatcatc tactttgatg acaaaaattg
    12001 ccatcgccgg aagattactt tctgaaaaca aaggaattga ttcattagta aaatacgtga
    12061 tttcaaacaa aaacataaag acaattatca tttgtggaaa agatactgct gggcataaac
    12121 caggacattc gcttcttaat ctttatagaa atggcataga ccataatgga agaataataa
    12181 attcgtcaag tccggatcca atcttaacac taacaaagac agaaatcatg acatttcaaa
    12241 cgcaagtcaa actcatagat gagatagatc aaaccgatat cactaagatt agaacgttaa
    12301 ttgattcagt gatatctgtc tagtaaccat ttttttgcct ttcttggttg atcttgttct
    12361 ttttttgata ttcattgaga atgtcttctg gtgtcatgtt caattcaagt gatgcttgga
    12421 ccacaaaatg ccatatgtca attagctctt cacgtgctgc agcctcatca aaaaccacag
    12481 gtgttttcca ccacttccag tttgttgtac gctgaagttc tattgcctca tgtattatgg
    12541 cagtacaaag agcagaaatt tttccttcta catcctttgg atatctatct aacttcatca
    12601 tcttttcaag atttttttgc atcttgaaga ttgattctag cctgtcatcc atcatttttg
    12661 atttttattt gacatctata acctttgtta gaagcgtacc attttcttat attgattgaa
    12721 tctttttcga atttttgatt ttcttaatct agttaatcca tccaatccgt attcttcaac
    12781 agcaaacgat cccattatgt ttccatagac tgcagcttcc ctaatagttg aaatctttgt
    12841 gttgttcttg ctagacatat atccaatcat gcctcctgca aatgaatctc ctgccccagt
    12901 cggatcaaca atattttcca tagaaaatgc tggggaggga aaaaccaaat cctcgtaaaa
    12961 aagcaacgat ccatgttcgc cttttttaat aatcacatat cttgcacccc attccatgat
    13021 tttttttgca cattttatca agttctgtgt tttggttaga agttttgctt cttcgtcatt
    13081 tattactaca gaatctacta ctcccatcat ttttatgaca tcatttcttt ttgttgaaat
    13141 ccaaaaatca atcgtatcac acatagaaaa cttgacctta tcaaattctt ttataatttt
    13201 ggtgttttgc acaggatcat tatttgccag ataaacaaat tcggattttt tgtattcctc
    13261 tggtacagta ggcttgaagc ttccaagaac attgagttct gttttgtttg ttgttctatg
    13321 actgagtgtt tcatcataac ttccatcata cctgaatgtc ttcccatctg cacgctcaag
    13381 tcctctcaaa tcaagatatc tgcctagagt attgtggtaa tttttcggga aatcttggcc
    13441 tacgactgct atcaatcccg gtttaacaaa aaaacttgct gaaattgcag caaatgtagc
    13501 cgcgccacct agaacatttt tcaaaacctt cgtaggcgtt cttatggtat caagagcaac
    13561 tgatccaaaa accgtcagca tgttttgctg gttttactga ccgtgtataa attctctggc
    13621 ttcctcttgt gttggataat atgtaaacgg aatgtcaagt gtagtaaaca acagattgta
    13681 tcttgttatg ccgctaagct tcacggatgg agacacttca ctattatcat atttgtcaag
    13741 cactgcatta tctgcaagaa agggcgcatc tactacaatc ttgcccatag caggtattac
    13801 aaaaggttct aaaactcctg cacccagatc ttcgtcttct acagatatca caaagtctgt
    13861 tttacctata ttgagaatca taaatgatgg attttcaaac tctaattgca tctgatacag
    13921 tgaacctgaa cttgttcgtt ctacaatttc actgtcctta atcgatacat ctagctgcat
    13981 tgcagaagca tattgaagat atccaaacac accaataatt actgctatgg ctattgctat
    14041 tactgctacc ttggactttt tctgcatatt tttgattaat ggattacctt ttagcacact
    14101 tggggaaaaa aatcaaaatg acatgtgtaa aatttttgat ctactttcta agtagcttag
    14161 agctagtagg ataaatgaaa atacacaaat gagtatatga aatggctaaa attagactaa
    14221 aattcggcgc aaatgagatt gaagtagaat ctaaggactt ctacgtagac aaccaaagca
    14281 tagacgaggt tgtaaacaat ctatctagat atgtctctga aaggacaagt acacccgagt
    14341 acgtctcatc tgaacaaacc caatcgacaa attatctaag ctcgcttgaa gatgcagaaa
    14401 ttcacgaacc agagtttgtt agacccatat ccattgatac aactcaaatt aaaagcaaaa
    14461 taaacatact ttctagagat tctttttttg aacaacctag aacagtttca gaagtagttg
    14521 ctcagcttta cgaatatggc tgggctgcaa gacctctgga tgtctcaaaa gtgttggcaa
    14581 acatggcttt caacggagaa ttacagaaag actcacgaga acgtagaagt tactatacta
    14641 acaaaataat gcaacttagt tagtggattc ataactgcac gtcttacagg aatcaaatac
    14701 ttctccctcc aaatgatcaa agtgagcatt gcatttttta caagtgtgat gcatatcata
    14761 atacccatca ctttctgtct ttccaatctg atttgatatt aattcagtac ttccgcactt
    14821 tatacaatgg cagccacatt gcatgttgtt tggtaaaata ataccactaa taatctagtg
    14881 gaaatggaat ctatttctga actgaattaa aataactgca gaaattccta ttacaagtgg
    14941 aacaaataat ggaaattctg ggatcactgt tgttcctatg atctgcacat ttccagatgt
    15001 ttctggcttg aatccaatcc aaacatgtgt tccgttattg taaaactcag agttttctgt
    15061 cccgttgcta tttaggtaca catcatacgg ctcccatagc agttttagag gaataattgc
    15121 cgtagtgtac gaaccgccat ccgaaatatc aaaactaagg ctttttgatg actgatcaaa
    15181 ttcaaacatg tctatactcg taagggttct gattcctaca gtgaattttt cattttccca
    15241 agtcgtttct tttaaaatca taggttcatc agcgatgtat tctagtttca ttgcaccacc
    15301 atgctgtcgt attccttttc ctgcaagata cactggattt tcgtttaccc aaataatatc
    15361 aacatttcct ggaaagtaga aattagttat ttcttggcca gtataatccc aagtccacac
    15421 tccatttctt aaagacaaaa catcagtcaa gtcatacctg atgaaagtca tatccttgtc
    15481 tggcggtaac actacggcta tgggttgttt ctctaaagta aaatattgta catcgccacc
    15541 attctcattt gttactgaga gattcgacat tgttccattg tatgtttcaa tctgctgggt
    15601 ctttgtactg ccttccactt tgtgtactac gtgagctatt ccattgacat caattgtaat
    15661 ctctgttatc tgttttggtt tttcaccaaa agttatatcc tgaccaaata cttgtgacat
    15721 ggaaaaaagt aatcctgaca ttacaacaat tgtaagaatt ttgtgcaact cgatctccta
    15781 ttctgatatt gttacatgaa gcatttttgc ttcttggtgt ggacagtcgt ttttatctct
    15841 aggcaaattt actatcttgt ctgctacatc cattccttct gtaacttgac caaacactgt
    15901 gtactgtcta tcaagaaagg tactatctgt agtaactatg aaaaactgtg aaccagcact
    15961 atttggatcc attgcgcgtg ccattgaaac tatgccacga agatgtgatt ttgaactaaa
    16021 ctcagctttg atagtgtgac ctgggccccc catgccccac ttgctcttat ctggtttctt
    16081 ggtgttggga tctccacctt gaatcatgaa tcctggtatc actctatgga ataacgtacc
    16141 atcataaaac gaacttttgg ccagctgtac aaaatttctc actgtctctg gggccaaatt
    16201 tggtaaaaga tcaaacgcta ttttgccaaa atttgtctct attgtagcct tggtcatttt
    16261 ctgcatttgc ttaacttaat cataagagtc tttcttcact aaatctgcct cgcaattagt
    16321 ttagcagact gccttttaga ttatttaaga gaaatttttg agattattat attgtttaat
    16381 agaatttcta tagatctgat aaattacaat gacttgtatt ttggtgtctg attttgaatc
    16441 aagtcgaaat attttccatt ttaaatatgt agtaattcta caaaaaatac agtaacagtc
    16501 ttatatagaa gatctgaaaa ttaaaaattg tgaatcgctc tactcaaagc acaaacataa
    16561 aacaagcaat caatgcgtta ctcgaccaag gggttacaga taaacaggat atctatacta
    16621 aagtagtaga agagcttggc gttccaagac cgactgtaag gcgagttgcg cgagatttac
    16681 gaaacgaact tttacaaaag attcagattt tgcaatccga ggttccaaga cctggatact
    16741 ttggcaataa agatactgaa agccttactg cctagcaaaa tctttccttt ttataattaa
    16801 ttttccaaac ttgatacttt ccattactat tatattcaag caaatttgga tgtgaaacat
    16861 gggctatctt ggtaatcatc tagccactgt agtaactagt atttttgcag cgaccttgac
    16921 tgggctgtat tttgtacttc ctcctgctac ccactttatt ctaataatcg caataattgc
    16981 aacatggttt ttggctttta tatgctggat tgctcaaaaa agcaatgact acatgcacaa
    17041 gcatggctct catgaagtaa aaaaaaagct aactgaaaac accagctctc atgtacaatc
    17101 agcacaaata agttctgagg aactaaacaa agctagggta gagctctcaa ctgatcttga
    17161 tgcactcaga gacaaagtaa tgatcaagga caaggaaata gaaaacctca agaaagaaat
    17221 ttcaaatctc aagactctgg tagaaattga atctatccga accgaacttg caaacctaaa
    17281 gatgctggca tctcaagaga actcaaaacg taaaacaaag aaaaaataac ttatttacaa
    17341 tgttcacaat gatgttttcc atgatttttt ttacagctag gacaatctac agctccacca
    17401 aaatggcaac gacatttaca tccatcattt agtttctttt ctataatcaa tttctatgaa
    17461 ctaatcatac gtcatcttaa tatgtgactt ttgttgtagg gcttgcatga aatccgtaat
    17521 gggtttgggt tttgcagcct ttgctgtatt gtttgtaatg ttacccagtc cgacctcatc
    17581 ggcaactgat gaatttccat tagaaccaca acttgtggca gataataatt cagtcatatt
    17641 agaaaagaaa atcatatcaa tgcatatatc agaagataat caactgccat ggggatttgt
    17701 tgaaggaaat gtaatcaatc ccgctgaagg atatcctgta ataatccaga tatacaaaaa
    17761 tggtgaacca gttcactttg cacagactga tgtccaagaa gacggctcat atgaatacag
    17821 atttagagta agaaacgtag acggtgatca agtgatcaac atcttccagg gtgattacac
    17881 tgtcaagatt ttcaagacag tttatgcaac tccgcaagca caaatagttt aagaagcaag
    17941 ctttagcgtc tcatcaacaa attcgcgtag tctaaccatg gcattctcat caacaatctt
    18001 gccatctttt atctgatctg ctgtcatatg aactgctttt gggtttgtta tgactctaaa
    18061 atacgtcatt aatcccgtaa ttacattctg tacatttgtg aagctgatat ttcctgaacc
    18121 caatattgca actcctgcag tcttacctgc agtttccttg tagttgatgt actcaaagag
    18181 attttttaga gcagaactaa aaagagaatt gtatacgggc gaaccaacaa accagacatc
    18241 agcttctgta acatcctttg ccgcttgtat tgtggtttgg ctatattctt ctcccggtcc
    18301 tcgataacaa tccaccatac cttctgatag atcgatgaat tttgtatcag gattttttgt
    18361 cttggcataa ttatacacat attccatcat gatcttagta ttggcacctt ttctaggact
    18421 acctgagatc actacagcct tcataataat ttcatcaatt gatattgtat ttaaatcaaa
    18481 taactaagaa tttgtatttc acgtttgtac actttttaat taattggcac aatgacaata
    18541 acgattctta gcatcttggt tcatgtgatt atgctgcaac tgaaatttgc ttttcagatt
    18601 ctttttcttt gagatctttt tgttttagtt ttgcaatata ttcttccagc atcgcatatt
    18661 caatttcatt acacattaga taccaataca cttgaaagta taaaaaatct ttattgttaa
    18721 aatgtggatc gctgtttatt attaatcaat taaagcgggc tcggagggct tcgatccctc
    18781 gacctgtaac ttaggaggct accgcgctat ccatgttgcg cgtcacttag actctgcgcc
    18841 acgagcccaa cgaaaatcaa agctggtaac aatttaagcg taatcaagat ttgagattat
    18901 catttgatga atctgattcc agcttggatc ctcaactttg ttccattttt cataaaatac
    18961 aacatctttc aaatcaagcc ttggtagcca tccgactgtc tcaagatctg gcttttctgc
    19021 aaacttggac acatggccaa gacaaagata tgcaatagga acaacatgtt ctgggatatc
    19081 caaggttttc ttaagatcat cattagaaag aatgctgacc caacccaatc caatatcctc
    19141 ggttcttgct gcaagccaaa gattttgaat tgcacaacag acgctgtaaa ttcctgtttc
    19201 aggaatactt gatctgccaa ttacaaatgg accaaacttg gaaggatcat aagtcacaca
    19261 aatgttaacg gcagaatcta aaattccttc taatctcaaa gaaagatatt ttgatctttt
    19321 tggatcttca ataagagccg atgagcgttt tctttctacc tcaaaggatt ctttgatttt
    19381 tttccttgta ttttgatcct taatgagaat aaaattccat ggttgtgaaa agccaaccga
    19441 cggtgcatga tgagcagcat ttagaatctt tgataaggtt ttgtcgtcta ttgggtctga
    19501 tgtaaaatga gatctcacat ctctccttga aaaaactgcc ttgtagaatc catttttttc
    19561 atcctgtgaa aaatcgtcat tcatgaatta accattaaga acaatgcata ttattcttgt
    19621 tttctgaaat taaacgttaa taacgtctct tagaagagtc gtattctatt gggccgatgg
    19681 tctagcctgg taggatacct ccctgacacg gaggtggtcg agagttcaaa tctctctcgg
    19741 cccacttgtt tttattatga aaagatcaga tgataatatg gtaaaattta ctcaaaaaga
    19801 aattgacttt ttacaaaaca atgaagcatg tagaatagct acctcacata acgatatacc
    19861 acatgtagta cctgtcactt tttactttga gaatggtaat ttttacatag cagtagacta
    19921 tgatacaaaa aaatattcta atcttaaaaa aaataataga gctgcaatta ctgtagacat
    19981 ttatgcagca ggaaagcaca aagcagtaat tgtacaaggc gcaacagagt ttatagaaaa
    20041 aggctcagag ttcaaacggt tgtatgaaac ttttcataaa aaattcagtt gggtacgtga
    20101 aaatccttgg aaagaaggtg aagctccatt tgtcaagctc atgccaaaaa caaagacaag
    20161 ttggggttta aactagttta gagtagactc gaactgtttt tcaactatgc tccaagctga
    20221 ttcaacacta acttggccag ttattttata gcttacatgt tcgccgttta ttatggcaga
    20281 atatacatca gcaacttgat ctgtatatac aagattcaag gtactgggaa tctcaattga
    20341 ttgtccagga tagaacgctg ctcttcctgt catagctacg tcttctgtac tgtattcgcc
    20401 atttccaaca ggtctgtcgt ttgcaaacaa gacgtaactg atgttagata gggtaagagt
    20461 tctttgacta ggatttgtta caagaaagac aacttctaga tctgcgcgtt tgtctatgct
    20521 gttagtatca agcaccttga cactaactaa atcgacttgt acttgttcca gctgagggtt
    20581 gtctaaactt gcataatata caaatcctaa cattataaga ccaagtccaa taaacgcccc
    20641 aattatggag attctacctc tactaaccaa attcaacagc tacgggtttc atataactaa
    20701 aaaaccttct tcgattaaca gaagttttga tgcgaacaga taatattcat aaaaattgat
    20761 agattggact atgccaattg ctgacgctct cattacttgg gcgcatttaa tttctgcttc
    20821 tatctgggtt ggaggttcta tcttcattgg aatagttcta gctccaatgc tcaagacaat
    20881 ttctccatca gtagaagaaa gattgaaaat aatgattcga gcaggaagaa aatttaacaa
    20941 aattgctgta ccctcattag ctattctgat tgctactgga ctttacaact cgtacaatat
    21001 tctcaataga cccgacatgc ttttttcaac aaattatgga atcatactta caataaaaat
    21061 agttcttgta atatctctga tcgcagcttt tgcagctcat accagaataa tcagaaaaga
    21121 gatcgagaaa aaaattgaaa caaaagaaat gtctgaagac tcgattcaaa aactgcgctc
    21181 caaaataata ctgctgggaa aaattattgt ttttatctct gtagccatac tactaatggc
    21241 agcaatgtta gatgctggaa tttgaatttc cagttattct gacttcaaaa ccattgtcaa
    21301 actttcctat tccatatttg caattcaata tttctgatgt aacaaacaaa ttcgtttcaa
    21361 gatgctttgt aatcttgtcc actctaaaca ccgaagtgcc atcggcaaga ctcatgggaa
    21421 gtactaacat atcagaaaga aactgatcta cgccaaactc tgattctaag aatcgctttg
    21481 ctattgattg accaattcca tctacgccct tgttgtatat ggaatcagaa ccattaacag
    21541 agctttcatc atgcgcatat gctaaaaatg aacatcctat atcataggcc aactcatttt
    21601 ttatctcata ttggcaatca aatccggatt tttccaaaat ttctttggct tttgttatct
    21661 catgttccat catttgtgat gaaatcatag aatgagagca agtgatcttg actgatttgc
    21721 tagttctttt tagaagagaa attggcttga gcttttcaca tggaaaaaca tctacatcca
    21781 ccatgcctcc cccacgtgga tagtatccac gttttttgat atcgatggaa aaattaattc
    21841 ccattctaga ataggcctct cttaaaacaa atcttgtata atcactagtt ggactccata
    21901 gcacatcagt gccgccagtt attgtcatct ttaatttctt ttttgaaatt gctaccgtag
    21961 gaatcaagac ttgcaaaatc agcggtatac ttccagcagt accgacgtct tcttttagat
    22021 ttgtatcaat aacgttagaa ggaataaact tgattgctgt agaaccaaca tgaagtcctt
    22081 ctactttggc attgcagatt tttgcaagaa tcttaattgc cgtcatgtgc tgtgccctaa
    22141 gacctggaac ctttctattt tttctaatgt tagtgatctc tacgggtttt tgagtcagac
    22201 aggaaagtgt tactgcacta cgaataattt gtccgccgcc ttctccaaac gcaccatcga
    22261 tttctattgg tttcatacta ttctagttcc ttggcataat aggatgtgat aatcttttaa
    22321 acaatcaaac aaaatttcaa tcatgaatgg tcttttcatt ggacggtttc aacctttcca
    22381 tcttggtcat ctggatgcta taaaatttgg actctcaaag atagaaaatc tttggatagg
    22441 aataggaagt tctgacaaaa gtaatgaaaa aagaaatcca ttttctgcag atgagagaaa
    22501 acaaatgata ctttcttcgc ttgatgatga tttcttgaag agaatcaaaa tctactatat
    22561 ctcagatgta gacgaccatg aaaaatggac atatcatgtg gattctttag tgccaaaata
    22621 tgatgtcgtg ttttcaaacg atgactttac gcacacactt taccaaaaac gtggaatcaa
    22681 agtaatctct gttcctttaa aagagcgaga aaatctttct ggcaccaata tcagagaaat
    22741 gattgcaatg ggaaaagact ggatggcact ggtacctgag ggaacaaaaa atgtattgct
    22801 aaaaatggat gcagaaagac gactctcaaa aattctttaa ttataaataa cggctgctaa
    22861 tcgtaaagat tggagaattt atttggaata tttggttatg ttgcccggac caacaaacgt
    22921 tccggaaaga gtaacacgtg caatgtttac tcacatgata aatcaccgaa gcaaagattt
    22981 tgttaaacta tacactgatg ttgtagaaaa gagccagaaa gtctttgaga cttcgggcga
    23041 tgtcattgca cttagcgcat ctggaaccgg cgcagtagaa gcatcagttg taaatctaat
    23101 aaagaaagga gacaaagtga taattcctgt aaacggagag tttagcaaca gattagctac
    23161 cctgttagag tggcaaggag caaatgtcat caggcttaca actccttttg gagaaaatgc
    23221 aagttttgat caagtaaaag aggcttttga taacaacaag gatgtcaagg cgttttatgt
    23281 tgtatggaat gagacctcga ctggtaccat ggtaaaatat cttgataaaa tatccaagct
    23341 cacctcaaga aacgattcat actatgttgt agacggtgtg tcaatagttg gtggtgaaga
    23401 attacgcatg gacaagtggg gcatcgatgt gtgcgttacg ggagcacaaa aagcactagc
    23461 tgcaccgcct ggaatctcac caatagcaat cagtccgaaa gcaaaaaaat acatgattgc
    23521 aaatccgcca cccacattct actttaactt ggcaagatac ttcaagtatt atgatgatac
    23581 aaaagaaact ccgtttactc ctgcattgcc acttctttac gcatacagag aagcacttga
    23641 tatcatatta gaagaaggaa tgcaagcaag aataaacaga cacagggttt gttcaaatgc
    23701 attctattct ggtttgagtg caatgggcct tactccattt gcaaaagaag atgcgcgatc
    23761 tactgttgta attgcactaa actatttgca aggattagaa gacaaaacat ttagatcaac
    23821 actttcagaa aaattccgcg tacttgttgc aggaggattt ggagatctta aaggtaaagt
    23881 attcagagta ggatgcatgg gtgaagttca caaatatcat gtgatgagaa ccatctctgc
    23941 aatttcatca actcttgaca tgatgggaca caagacaaac ccagaagcac tcaaagtagc
    24001 tgaagaaaaa ctaaaagctc tctaacctgc tgttaactta atataaggaa ctttgaacat
    24061 cgatcacgat gacttttcaa aaaggcacac tggtcttagt ggattacact gccaaggtaa
    24121 aagacactgg cgaagttttt gataccacaa gagaagcaga tgccaagtca agttcacttt
    24181 atgataccaa tgcaagatat caaccaagac tagtctcagt tggagaatca tgggtcctca
    24241 agggactaga cgacgcgtta cttgacacca aggttggtga caaacttaca gtcgaagttc
    24301 caccagagaa aggatttgga acaagagacc caagcaaagt cagaatgatt ccattacgaa
    24361 agcttggtga agacgctgaa aaagtctcag ttggagacgc aatagatgta gatgaaagaa
    24421 ctggaattgt aagatttatt ggatctggca gagttcaagt tgattttaat cacagatttg
    24481 ccggaaaaac cattctttat gacgtaaaca tagtaaaatc tctagacacc gacaatgaca
    24541 aaatcttggg actcttaaaa agaagattac ctgttgatga ttccaagctg caatttaaga
    24601 taaatggaaa agagattgac attaccatgc ctgaagaaac atttcttgcc gaaggactgc
    24661 aagtaataaa acgagcaata gtaaatgaca ttttcaaatt tgtacctgcc ttggataaag
    24721 tcaactttat cgaaggttac aaaaaggcgg aaaagactga agcaaagcaa gtcgaatcaa
    24781 aacctgctga agcaaaagct gcaccaccaa aagcagcctg atctataaaa caccagtgct
    24841 ttttgcaagt gaaatagctt tttctcttgt catttttatt ttatttaaaa ttgtgtatct
    24901 ttcttttcta agtgatggtg ccattaccag agcttttgca agcatgtctg gtttaagacc
    24961 gatttgtttt gcagtagtgg gagctccgac actttctagc gttgagacaa ttcttttcca
    25021 gtcctgtcct tgcaatttgg aaattaggat ggctcctatt ccacacttct caccatgtaa
    25081 cccaactcca ggaaccaaat aatccaaggc atgagaaaaa agatgttctg caccagaaca
    25141 tggtctgcta cttcctgcaa tacaggcggc aacacctgag ctaatcaaag cttctactat
    25201 catcctgaca tctggttttt ttcctgattt tgaattttca attactattt ttgcactcat
    25261 tgaagcaagg tttgctgcat atgttccaaa atactctcca acatcatctc tagcaagttc
    25321 ccagtccctg actgcagtta tttttgccat caaatcaccg catccacttg caagaagttt
    25381 ttttggtgcc ttttttagaa cttcgatatc tacaaagact ccaagtggtg cagttgcaac
    25441 aatcgaatgt ggtttatcac cacgcaagga aacaaaagga cttgcaattc cgtcatgtga
    25501 tgcagaagtt ggtatgctga caaatggttt tttcaaatta aatgcgatca tttttgcaat
    25561 atcaacagat ctgccgccgc caattccaat aattagatcg cttttgtcct tgcttatgct
    25621 ctgttgagtt ttttttgcag tttttacatc attactggtg ctaatgtgcc agacattttt
    25681 tatctttgat tttaaaagtg attttacaat ttttgtttgt acaatttttc ttacttgttc
    25741 cccagaaaca agagaaacct ttctaggatt ggcaagatca gctataaatt ttccaacatc
    25801 tgatatgttt ttttcaccaa tgactatctg tcttgggagt tccatcacat gcgaggacat
    25861 gggtttttga tgaaatttca ttatttatca atctcccgtt tgggaaacac aaagttattt
    25921 aaaagggtgc tagcctatcc aaggcatctt tagttaagga gaatgttatt tgtcagacta
    25981 tgttgaacag cacacactac atggaacaac tactgtagga atcagttgta ctgacggtgt
    26041 agtattgtgc gcagacatga gggccagtgc aggttatttc atagcaaaca ataacactat
    26101 gaaaattcag aaaattgact accacgcagg aatgacaatt gcgggaggag ttgcagatgc
    26161 acaaaacgtc actgacatgt tacgatacca ttccaacata catagaatag aaaaacagga
    26221 atatcttccg atcaagtcca tagcaaggct agcatctcta atcttcttcc aaaacagata
    26281 ctatccattt gttgcagaca ttcttgtagg cggttatgac aagcatggac catctttgta
    26341 caacattgac atgtttggct cacttgacaa gaaaactttt gtgacaaccg gaagtggttc
    26401 tcctgtcgca tacggactat tagaaaacga gtaccgtgac ggactcacag tagaacaagg
    26461 aaaaatcata gcattacgtg cagtaaaagc tgcaataaca agaaacattg gaactggaga
    26521 cggaatcaat gttgcaataa tagatcagaa gggataccgt ctgttaacaa aagaacagaa
    26581 aagagccata attacacttt agtgatattt tgcaaagaaa acaacaacaa cgagatctaa
    26641 ccccagccac aaatataatg ggaaccatac tgcaaagtat tccaaaagaa gcggatgtga
    26701 ccaagataga ttatgaaggt ccaagaattg caatctatac aaaaaaaccg cgttatctaa
    26761 tggagcacaa tgaaattgtt tcaaacatgg tcaacgtgat caaaaaaaga attgttgtac
    26821 gaacagaaga atcgataaga aaaaccgaag aagaggcaag acagatactc actcaagtcc
    26881 tgccaaaaga agcagatctg agaggaactt tctttgatac tgccataggt gaagtcactg
    26941 ttgaggtaaa gaaaccctgg cttttgttta acaatgaagc gtttaacatg gcagaccttg
    27001 ttgaaaaaag tggctggaga tttcgaataa gaaaggccac caaaattcaa tcacaaacaa
    27061 ttcaaacaat aaattataat ctaaaaatct catcttcaga aagaagcaag catctaaaac
    27121 aagtcggtga acaaattttc agaccaaaac tttctgaaac tgccgaagtt tctttgatga
    27181 ctcttggtgg attcagccaa gtaggtcgct cttgcatgct tttgacaaca catgaaagta
    27241 aaattctaat cgactgtggt gtgaacccag gtgcaaaaag tgcaatggaa gcatatccaa
    27301 gacttgattg ggcaaacata acactagatg agcttgacgg agttgtaatc agccatgcac
    27361 atcttgatca cactggattt ttgccagcgt tattcaaata tggatacaag ggacccgtat
    27421 actgcacaga accaacctta cctatgatga atctgatcca gcttgatgca atcaaggttg
    27481 ctgcggcaca aggaaagata cccctgtatt ctgaaaggga tgtaaaacaa gtcatgaagc
    27541 aaacaataac gcttccatat ggaactgtta ctgatatttc tccagacatc aaacttgtat
    27601 tttctaatgc aggccacatc ttgggttctg ctacttgcca ctttcacatc ggaaacggtg
    27661 atcacaactt tgtttacagt ggtgacttga aatttggaaa aacaatgctc cttgaaagtg
    27721 cggtatggaa ctatccaagg gtagaaactc tactcattga aagcacatac ggtgcaaaag
    27781 aagacatcca gcctacacgc gaagaggtag aaggtgcctt tatcagatct gttaatgaga
    27841 ctctcagaaa cggaggcaaa gtgcttattc ctatacctgc agttggtcgt gctcaggagc
    27901 ttatgatggt aattgacatg tacatgaagg caggtcagat agttgaatcc ccagtcttta
    27961 tggaaggaat gatttctgaa gcaacatcaa ttcatgaagc atatccagaa tatcttgcac
    28021 gcgagctgcg acaaaaaata ttggaaacag atgacaaccc gtttgattct gaatatttca
    28081 caaacattga acatgcagat gcaagagaag aaccgttacg ggaaggacct tgcattataa
    28141 ttgcaacatc tggtatgctt gaaggtgggc cagtcttgga atatttcaaa aacattgcac
    28201 catcacagaa aaataaaatc ttgtttgttt cttatcaggt aaacggaaca atgggaagaa
    28261 gagtacttga tggcgcaaga caagtctctg taataggaaa agatggaaaa gtagagatca
    28321 taaacatcaa ctgctcaaca gaaaaacttg atggattcag tggtcacagc gattacaacc
    28381 agctaatgtc ttttgtacac aaactaagac caaagctaag acgagtcata gtaaaccacg
    28441 gtgaaaggag aaaatcagaa aacctttcca tgtctattca cagaatgttc agaattatga
    28501 caacctgtcc gcaggttcaa gaagcaatca aactatttta gatcgtattc tgctctccag
    28561 atttttacac ctggtggcgt gacaataatt ctttcttcac ctaatcttgc aatgtctgca
    28621 ccgatgcttt ttgcaattgc atagatttcc tcaactactt ttcttaattc ttctacattt
    28681 ttttgtgcaa gcggcgtgac tctgagaacc agaatcatgt tttttttgac atcctctttt
    28741 actgcatgga catcgctagt gtctcgtagt gtgatggcct tgagatacgt aggtgccttt
    28801 tgagcttgca tacgaatcta ctgcgaggaa ctccctcaaa aagtcttact agtcaagacg
    28861 aattcacaga ctacgttgca cgtctaaatt ctgtattgta ggtatgctga ctcggaagct
    28921 ggtattgccg aaatttcaga gcttgtgccg tttttctgga cagtcacaac actcggagtg
    28981 tctttaggag catctccata tcttagtgtg attgtagctc caaactctac atggcttgat
    29041 gcattttctc ctcgaattaa tgtgacagga ccgacatgat cttttgcttc aagcaaaatg
    29101 tctccatcta gtgctagtgc ctttaagact tcgttttcat cttgatttct tccaacgatt
    29161 agctttgtct tttcatcaaa tctgaaatgt cttccaattt ttagtaaatc aatatcattt
    29221 gttgttggag tttgtatgtg attgaaaaga tcttttgctc tgatagcaaa agctgggtct
    29281 gttagtaagc aacctccgcc tgcatttggt ggattttcaa ttccaaactc ttgtgccatc
    29341 tttaactgat ctttccttga tcgacctctt atttttccaa gatcttccct tttgatcaat
    29401 ccatttagtt ct
//
LOCUS       DATIDL010000161         2917 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrosopumilaceae archaeon isolate SMAG_U16342
            k141_199511, whole genome shotgun sequence.
ACCESSION   DATIDL010000161 DATIDL010000000
VERSION     DATIDL010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935836
            Sequence Read Archive: SRR25158326
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosopumilaceae archaeon (soil metagenome)
  ORGANISM  Nitrosopumilaceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrosopumilales;
            Nitrosopumilaceae.
REFERENCE   1  (bases 1 to 2917)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2917)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/04/2024 05:09:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,520
            CDSs (total)                 :: 1,474
            Genes (coding)               :: 1,474
            CDSs (with protein)          :: 1,474
            Genes (RNA)                  :: 46
            rRNAs                        :: 1 (5S)
            complete rRNAs               :: 1 (5S)
            tRNAs                        :: 43
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2917
                     /organism="Nitrosopumilaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_199511"
                     /isolate="SMAG_U16342"
                     /isolation_source="soil"
                     /db_xref="taxon:2202732"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2175)
                     /locus_tag="VLB45_07555"
     CDS             complement(<1..2175)
                     /locus_tag="VLB45_07555"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HSD05584.1"
                     /translation="MEPKIKETAWNPEIEKQILKQWKESHIYDFKPEGDIFVIDTPPP
                     YPSGRPWHIGAAAHYAQIDMIARTARMSGKNVLFPIGIDRNGLPVEMYTEKKYNIRMR
                     ETERGEFLKLCATALDDLEAEMIETMTSLGLSGNFAEYYRTDSEQYRTLTQLTFIDLW
                     KKGLVYLANRPNNYDWVTGTTIADAEITYMDIPTKLVYMKFKIKDSDKSLIVASTRPE
                     LLCACQTIIVNPDDERYTEFVGKKAVIPLVNREVEIKTHPSAEKEFGSGTVMVCSYGD
                     QNDVALFRELKLQEIVAIGKDGRMTEVAGAYAGLKVKQAREKIIEDLQNQGFVDKVDN
                     INHRTPVSERSKIPIEIIPMEEYYVKQKDSVDKIREIGSKIDFHPSMHKQILMNWLDS
                     ISIDWPISRRRYYGTEIPIWYCKKCSEPHVPEPGKYYRPWKDASPITKCTKCGASEFV
                     GEERTFDTWMDSSVSPLYISKYKRDEAFFKKTYPATLRPQSKDIVRTWLYYTILRCEQ
                     LTGKSPWSQAWIMGYGVDEHGTKMSKSKGNVVDPLPVIQKFGADTFRFWSASEINLGY
                     DFRCSEQKIESTKKFLSKLWNVSRFLSGFPILKSVNLGAADKWILSELDKVVSECKKG
                     YAEFNFFIPATALREFTWNIFAAHYIEMTKGRAYGAGFTEEERDSAIHTLHKVLSTIL
                     LLLAPITPFITDYLWKNLYSDKSIHKERFPEQENHEDWTRFTK"
     gene            complement(2180..>2917)
                     /locus_tag="VLB45_07560"
     CDS             complement(2180..>2917)
                     /locus_tag="VLB45_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007551305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HSD05585.1"
                     /translation="SIAEMLSFMDIKYEYDYKLTLKNGKQTTVDFKTEKGLIEVIDEE
                     SDIVKYKEIKNSLTDTKIIAIGHPKYAARIKELDDVILYKTKEVQTGSIFIEDPSFAF
                     DYAHILPLVEKCSILHGHTSSVMVELVGEMKNNLLIDFSDAKKIIKEIISILDHKFFI
                     SKKYLVKEDNLHYKIAFDGPKGVFDLQVPKNTTYLLEGEATVENLSTEIIKLLVPKMP
                     QNIEAVGVYIYEGYNKGAHILSHISKS"
BASE COUNT          777 a          585 c          492 g         1063 t
ORIGIN      
        1 ttttgtaaat ctagtccagt cttcatgatt ttcttgttct ggaaaccttt ctttgtgtat
       61 ggatttgtct gagtagaggt tcttccatag ataatctgtg ataaatggtg tgattggcgc
      121 aagaagaagc agtattgtag ataacacttt gtgcagtgtg tgtattgcac tgtccctttc
      181 ttcttcagta aatccagcac cataggcacg cccttttgtc atttctatat aatgtgcagc
      241 aaagatattc catgtgaatt ctctcaaagc agtagcagga atgaaaaagt tgaattctgc
      301 atatcctttc ttgcattcac ttacaacctt gtctagctca gaaaggatcc acttgtcagc
      361 agctccaaga tttactgatt ttaaaattgg aaatccagaa agaaatctag aaacattcca
      421 caacttgcta aggaattttt ttgttgattc aattttttgc tccgaacatc taaaatcata
      481 gcccaaattg atctcacttg cactccagaa tctaaacgtg tccgcaccaa acttctgtat
      541 gactggcaaa ggatctacaa cattgccttt actcttactc atcttagtcc catgttcatc
      601 aacaccgtat cccatgatcc aagcttgtga ccatggtgat ttgccagtaa gttgctcaca
      661 tcgtagtatt gtatagtaaa gccaagttct tacaatgtcc ttagattgtg gtcttagtgt
      721 tgcaggatat gtttttttaa aaaacgcttc atctcttttg tattttgaaa tataaagtgg
      781 agaaacgcta gaatccatcc aagtatcaaa agttctctcc tcaccaacaa attcacttgc
      841 gccacacttt gtgcactttg taattgggct agcgtctttc caaggtctgt aatattttcc
      901 cggttctggt acatgcggtt cagagcattt tttgcagtac catattggaa tctcagtgcc
      961 atagtatctt cttcttgaaa taggccaatc aattgatatg gaatctaacc aattcattag
     1021 aatctgcttg tgcattgagg gatggaagtc tattttagag ccaatttccc tgattttgtc
     1081 cactgaatcc ttctgcttta catagtactc ttccatgggt attatttcga taggaatctt
     1141 gcttctctca gaaacggggg ttctatgatt gatgttgtct actttatcta caaatccttg
     1201 attctgcaga tcttcaatta ttttctctct ggcttgtttt accttcagac ctgcatacgc
     1261 tcctgcaact tcggtcattc gaccatcttt tccaatggct actatttctt gcagtttcaa
     1321 ctctctaaat aatgcgacat cattttggtc accataacta caaaccatta ctgttccaga
     1381 accaaattct ttttctgcag aaggatgagt tttaatttcc acttctctgt ttaccagagg
     1441 aatcactgcc tttttgccaa caaactctgt gtatcgttca tcgtctggat ttacaattat
     1501 ggtttgacat gcacacaaaa gctctggtct tgtacttgca actattagtg atttatcaga
     1561 atcttttatt ttgaatttca tgtaaacaag ttttgtgggg atatccatgt aggttatctc
     1621 tgcgtctgca attgttgtac cagtaaccca gtcgtagtta tttggtctgt ttgcaagata
     1681 aaccaaacct ttcttccata gatctatgaa tgttaattga gtaagagttc gatattgttc
     1741 tgaatcagtc ctataatatt ctgcaaagtt tccactaaga ccaagacttg tcattgtttc
     1801 aatcatttca gcttctagat catctaacgc agtggcacat aatttcaaaa actcaccgcg
     1861 ttctgtctct cgcattctta tgttgtattt tttttcagtg tacatctcaa caggcagacc
     1921 atttctgtca atacctattg ggaaaaggac gttttttcca gacattcttg cagttcttgc
     1981 aatcatgtct atctgggcat aatgtgcagc tgccccaata tgccaaggcc ttcctgatgg
     2041 atacggaggc ggagtatcta ttacaaaaat atctccttct ggcttgaaat cgtaaatatg
     2101 ggattctttc cactgtttta ggatctgttt ttcaatctct gggttccatg cagtctcttt
     2161 gatcttaggt tccatatcct taggactttg agatatgtga taagatatgg gctcccttgt
     2221 tgtatccttc gtaaatatag actccaacgg cctctatgtt ttgaggcatc tttggaacaa
     2281 gaagcttgat tatttcggtg gaaagatttt ctacagtagc ctctccctca agcagatatg
     2341 ttgtattttt tggtacctgt aaatcaaaca cgccttttgg accatcaaat gcaatcttgt
     2401 aatgcaggtt gtcttcttta acgagatact ttttactgat gaaaaacttg tgatccaaaa
     2461 tggagattat ctctttgatg atcttttttg catcactaaa atctattaat aaattgtttt
     2521 tcatctcgcc aaccaattcc accattacag atgatgtatg tccgtgaagt attgaacatt
     2581 tttcaactaa cggaagtata tgtgcataat caaaagcaaa tgatggatct tcgataaaaa
     2641 tagaaccagt ctgaacttct tttgttttgt aaagtattac atcatccaat tcttttattc
     2701 ttgcagcata ttttggatga ccaattgcaa ttatttttgt atctgtaaga ctattcttaa
     2761 tctctttgta ctttactatg tcgctttctt catctattac ctcgatgagg cctttttcag
     2821 ttttgaaatc taccgtagtt tgttttccgt tttttagtgt taatttgtaa tcatattcgt
     2881 attttatatc cataaaggag agcatttcag ctattga
//
LOCUS       DATIDL010000162         8645 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrosopumilaceae archaeon isolate SMAG_U16342
            k141_1814815, whole genome shotgun sequence.
ACCESSION   DATIDL010000162 DATIDL010000000
VERSION     DATIDL010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935836
            Sequence Read Archive: SRR25158326
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrosopumilaceae archaeon (soil metagenome)
  ORGANISM  Nitrosopumilaceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrosopumilales;
            Nitrosopumilaceae.
REFERENCE   1  (bases 1 to 8645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/04/2024 05:09:25
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,520
            CDSs (total)                 :: 1,474
            Genes (coding)               :: 1,474
            CDSs (with protein)          :: 1,474
            Genes (RNA)                  :: 46
            rRNAs                        :: 1 (5S)
            complete rRNAs               :: 1 (5S)
            tRNAs                        :: 43
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8645
                     /organism="Nitrosopumilaceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1814815"
                     /isolate="SMAG_U16342"
                     /isolation_source="soil"
                     /db_xref="taxon:2202732"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..558)
                     /locus_tag="VLB45_07565"
     CDS             complement(<1..558)
                     /locus_tag="VLB45_07565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05586.1"
                     /translation="MISIEADEKPDSNWNLRLLNSPHGTIYQTKEIAAYLETVLRSKN
                     IFLKFVNERGKIVGQLLLSTYSRFDKKGKVGRILKKIPGQKNLIYRWSYGPVIFDSSV
                     NNEVCAALHDFLILRNCRVLGYEHPLAGGSLSVLGKPFKIRPWATFLIDLSLNKEILW
                     KNMDKHAARKNIERSRSRGVYVKEID"
     gene            complement(622..2118)
                     /locus_tag="VLB45_07570"
     CDS             complement(622..2118)
                     /locus_tag="VLB45_07570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSD05587.1"
                     /translation="MSDIRVTYSGLIALVVGLVSVVTGIVFTLIVTRRLSPEEFGMWS
                     IIGSMISYFLIAEPVISFWSTRQIARGEEVGKTSLISSISFSMGAIPLYLVFAYFVST
                     VSRSHLDSLILASVLLPVTFVSQTLSGINLGHKPHATSYGLLVFEMLKIPAGLALVYS
                     LDLGVNGAIMATVIAYLGKILVQGHFGKTRLKDKFSINALKRWIKLSWIPLYSNMAHL
                     IWTLDVVLYSIIVGSVKGVAYFSVSSTIAAIIGHAGLISQALYPKLLAKGSHDYAREN
                     FTRLVYVAVPLLGVSVIFAKPALFALNPVYADGSVIVVIMSFRTFFYVITGVLYQMLL
                     GIETTDIEQNPKFSSLTKSKIFFVPTLTNIHYSLYLISMVITIIVLNSSGMSELELVT
                     WWTIIALALQMPFFIYAWILVQKYVKFSFPYVNTLKYVGSTILFVIVFLLTSEFIIRY
                     PISIYDFLPPLLLQIAICLGVYLLTTYIIDKKTRMLLKSILSEFGPNK"
     gene            2208..3344
                     /locus_tag="VLB45_07575"
     CDS             2208..3344
                     /locus_tag="VLB45_07575"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HSD05588.1"
                     /translation="MSKILCVFPNDPLSAYYKKGEIKPRYFNPKNIFDEIHVISLFDS
                     DEKEENVKNVAGEAKLKIHVVGKTTLLNVRSKKKEIINLIKEIKPDVIRSYNPLLQGW
                     IAVHAGKELGIPTVISLMGDYDRDLRYFARKNHDIKSYLKLLFTRRTLESFAIKNADE
                     VIIIYDFIRNYAKKMGAKNINLIYNRVDLTQFSPDTEPAFRESKPVVICVGRLMKEKN
                     QECLIRAIKDLDIVLLLVGDGSQFNELIELTKKLGIQDKVKFERAIPHKDIHRYYAAA
                     DLFALPIKYGGFAIPVLEAAASGLPVILPKQEFDPNPELIKDFAMLVNNDPQSFRDAI
                     SKILSDNQLREKLIRIGLDTVKKISSEVMEEKEKELYMKLLEKN"
     gene            complement(3327..4373)
                     /gene="wecB"
                     /locus_tag="VLB45_07580"
     CDS             complement(3327..4373)
                     /gene="wecB"
                     /locus_tag="VLB45_07580"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HSD05589.1"
                     /translation="MCIIVGTRPQIIKSQPIIKEILSHKSKLSIIHTGQHYDYQMSKV
                     FFEEMKIRDPDFNLEVGPGTPSGQLAQIIQKLEKPLQKIDPDVVLVPGDTRSALGAAL
                     CANRLGMKVAHVEAGARSMEFDMEEEINRRLIDHCSKILFAPTHNCYKNLKKESVLGS
                     TYFTGDTMYDVFLEFKKILNLTKTDSDKYVLMTLHRKDNIQNYQKIKKIIELAKQISA
                     KGWDVIFPIHPHTQKQIKSFGLNLNNINTINPVKYSEMLRLLCDANLLITDSGGLQKE
                     AYWLNTPCVTLRASTEWIETLDGKHNILLNVITNSSMNTIMKILKRGQLKTKSFTQFG
                     NGKAAKKITSILFQ"
     gene            4478..5218
                     /locus_tag="VLB45_07585"
     CDS             4478..5218
                     /locus_tag="VLB45_07585"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HSD05590.1"
                     /translation="MNDDYVRIKNKIMSSDPSRYWGDDFDVRFYLVSKLKKLYNKSVL
                     DVGGGIGIISSELDKSNFRINVDSSFCDLKTCRKKMDQDIQNICASMTHLPFIDNSFD
                     SVICSHVLEIAKHFDIEKNQMIIDNSIIQYPTVETTLKEISRVLNSGGKLFLTTPNNT
                     YYKSTKLNYHELKRSISNYFARYSLSFYNTFPRLSRKHRKLNLANILPKLMSNVISKN
                     KLITTSLIKEDRGTERESVSFYLEAYKN"
     gene            complement(5215..6390)
                     /locus_tag="VLB45_07590"
     CDS             complement(5215..6390)
                     /locus_tag="VLB45_07590"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HSD05591.1"
                     /translation="MNILVFCNVDLSQKWGDYTRVFSLMKAFSQLGNNVFIIIIRPEH
                     KTPRITSFKENGLDVTEIHPPKSLKFKGKKGLGRYLNYLACLPAISKTASEIIQKNHI
                     DYVYSYMPGIGSSLPAMRMKSKHKIRFILDYADLHVFVKPKRIADSSFKMADKILAIT
                     EYLRNELARKKTDSTKVYVVPNGVDLELFNPSKHDQNDIKQLKNSFGTNKLAIFSGSL
                     QDLNIIIDSATQVVEVISGIKYIIIGDHRDPSRSKSIWEEKVKKKGLSQYFEFLGRKP
                     HEEIPKYLLCADVCLDSFPDEPYYAAAHPIKLLEYGACEKPVVATRVSETEKIVKHGT
                     FGLLASPGNSSEFAQHMITLLNSQELREKMGKDFANYVRTNFGWNKIAADLQKILEN"
     gene            complement(6396..7343)
                     /locus_tag="VLB45_07595"
     CDS             complement(6396..7343)
                     /locus_tag="VLB45_07595"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HSD05592.1"
                     /translation="MKNFSGASILVTGGNGAIGSNLVRKLLNMNSKVVVLDDFSQSKT
                     GNLPSNRNLCVIKGDITNKKILSKVFSHQFDYVFHLAARFANELSIKDPLEDLRVNVQ
                     GTLQILMHASKQKPERLLYTSSSSIYGPQESTKLKESLILHPSTPYAASKLTAEHYCR
                     TFHELHGIDCTIVRLSNSYGAFDPPGKYRNVIPNFMHAAVRGKNLIITGTGNETRDFT
                     YVDDCVNGIILAATQKRGKNETFNLGTGKETPVRKIADMILKITKSRSKIVFKPMRSF
                     DHIKRRKMDISKAQKMLHYNPTTDIETGLAKTYQWFLNR"
     gene            complement(7370..>8645)
                     /locus_tag="VLB45_07600"
     CDS             complement(7370..>8645)
                     /locus_tag="VLB45_07600"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HSD05593.1"
                     /translation="GQVGLPTALTFCKSGFDVIGHDINEKLLSTLNSKKLPFEEDDLD
                     DLLKSSINKDKFHINNNLAESVKNSDVIIICVATPLTNGVRPDLSALENVCKSLSGLS
                     LANKLVIIESSIPPGTFAGYVMPSLGTKYRIGSDFWAAFVPERLAPGQALSEIQTTPR
                     VIGYVDEDSGLLAKTLYEKIVKSQIFLTTAQIAEISKLVENTFRDVNVAFANEVGMIC
                     EKYGVDVAELIRVCNSHPRVKLLQPGPGVGGPCLPKDPYLLLNPQGNSQIDSKIILES
                     RKINDSMPYHVVNLVTDALKAQNKKIAESTILVLGIAYKGNVSDTRFSPAKEIISYLL
                     KDGCKVLVFDPKTEESFGGQKITDIQNVISSCDAMIVVTDHNEFKKLDLKMIQKMKKT
                     PILVDTRRIFQRKEVEDLGIHYLSIGYKKPQT"
BASE COUNT         2817 a         1470 c         1425 g         2933 t
ORIGIN      
        1 atcaatttct tttacataaa cacctctact tcttgatctt tctatgtttt ttctagccgc
       61 atgcttatcc atatttttcc atagtatttc tttattcaat gaaagatcga ttaggaatgt
      121 tgcccacggc cggattttaa atggttttcc aagtacagaa agtgatccac ctgcaagagg
      181 gtgttcatat cccagaactc tgcaatttct caaaattaaa aaatcatgga gtgcagcaca
      241 tacttcattg ttgacagaag aatcaaagat aactggtcca taagaccatc ggtagatcag
      301 gtttttctga ccgggtattt ttttaagtat cctaccaact tttccttttt tgtcaaacct
      361 agaatatgtc gatagtaaga gttgacctac aattttgcct ctctcattaa caaattttag
      421 aaaaatgttc tttgaccgta atactgtttc cagatatgca gcgatttctt ttgtttggta
      481 aattgttcca tgaggagaat ttaacaaacg aagattccaa ttagaatctg gcttctcatc
      541 agcttcaata gaaatcatgt actacatttt tgctttacgt aatataacat taatagattt
      601 taagttgatt tgctagaaat atcatttatt tggaccaaac tcacttaata ttgatttgag
      661 aagcattctt gtttttttat caataatgta agtagttagc aaatagactc ctaaacaaat
      721 tgcaatctga agtagcagtg gcggtagaaa atcgtaaatg cttattggat agcgaatgat
      781 aaattcggaa gttaacaaaa acacgattac aaacaatatt gtacttccaa catactttaa
      841 tgtatttaca tatggaaatg aaaactttac atatttttgt acaagaatcc acgcataaat
      901 aaaaaatggc atttgtaatg caagggcaat aattgtccac catgtcacca attctaattc
      961 tgacatacca gacgaattta gaacaattat ggttatcacc atactaataa gatataatga
     1021 ataatgaata tttgttagag taggtacaaa aaagatttta ctttttgtca aactagaaaa
     1081 ttttggattt tgttcaatgt cagttgtttc aattcccaat aacatttgat ataacactcc
     1141 agttattaca taaaagaaag ttctaaacga cattatcaca acaataactg aaccatcagc
     1201 atatacagga tttaatgcaa ataaggcagg ttttgcaaag ataacagata ctcctagaag
     1261 aggtactgca acatacacca agcgtgtaaa gttttccctc gcataatcat gacttccttt
     1321 agctaaaagt ttcggataaa gcgcttggga aattaatcct gcatggccta ttattgcagc
     1381 tatagttgag gacacagaaa aatatgccac gccctttact gacccgacaa ttatagagta
     1441 aagcacaacg tctaaagtcc aaattagatg tgccatattc gagtaaagtg gaatccatga
     1501 tagttttatc catcgtttta atgcatttat gctgaatttg tcttttaatc tagtttttcc
     1561 aaaatgtccc tgcactaaaa tttttcccaa gtaggcaatc acagttgcca taattgcacc
     1621 gttgactcca agatcaaggg aatatactag agctagccca gctggaatct tcaacatctc
     1681 aaacactaac aaaccataac ttgttgcatg aggtttatga ccaagattta ttccacttaa
     1741 tgtttgactt acaaaagtaa ctggtaacaa tacagatgcc aatatcaatg aatccaaatg
     1801 tgaacgacta acagtagaaa caaaatacgc aaagacaaga taaagtggta tagcacccat
     1861 cgaaaaagat atgctagata ttagcgaggt tttaccaact tcttcgcctc ttgctatctg
     1921 acgcgtagac caaaaagaga tcacaggctc tgcaataagg aaataagaaa tcatacttcc
     1981 aataatagac cacattccaa attcttctgg tgatagtctt ctagtcacta taagagtgaa
     2041 aactatgcca gtcacaacac tgacaagacc aacaacaagt gcaataagcc ctgaatatgt
     2101 tactcttata tcgctcaatt acttcaatta agaaccttaa ttcataaata taaagaaata
     2161 aaatgctttt ttactcaaac agatcaaaat tcttgtgata aatcgttatg tctaaaatac
     2221 tatgtgtatt tcctaatgat cctttaagtg catattataa aaaaggagaa atcaaaccca
     2281 gatattttaa tccaaaaaat atttttgatg aaattcatgt cattagccta tttgattctg
     2341 atgaaaaaga agaaaatgtg aaaaatgttg cgggagaagc aaagttgaag atacacgtag
     2401 ttggaaagac aacccttcta aatgtaagat ccaagaagaa agaaattata aatttgataa
     2461 aagaaataaa accagatgta attcgttctt acaacccgct attgcaagga tggatagctg
     2521 ttcatgctgg aaaggagctt ggaattccca ctgtaatatc actcatggga gattatgaca
     2581 gagatctcag atattttgca agaaaaaatc acgatataaa gtcataccta aaattattgt
     2641 ttacaagaag aactttggaa tcctttgcaa taaagaatgc agatgaagtc atcatcatct
     2701 atgattttat tagaaattat gcaaaaaaaa tgggagccaa aaacatcaat ttgatttaca
     2761 atagggttga tcttacccaa ttttcaccag atacagaacc tgcttttaga gaatcaaaac
     2821 ctgttgtcat ttgtgttggt cgccttatga aagagaaaaa ccaagaatgt cttatcagag
     2881 ccataaaaga cctagacata gtacttttac ttgtagggga tggttctcaa tttaatgaac
     2941 tgatagagct tacaaaaaaa cttggaatac aagacaaggt aaaatttgaa agagcaatac
     3001 ctcacaaaga tattcacaga tattatgcag cggctgacct ttttgcatta ccaataaaat
     3061 atggtggttt tgccatacct gttcttgaag cagctgcctc cggtttgcca gtcatattac
     3121 ctaagcaaga atttgatcct aatcctgaat taataaagga ttttgccatg ctggtaaata
     3181 atgaccctca atcattccga gacgcaatct caaaaattct ctccgataac cagttgcgtg
     3241 aaaagttgat tagaattgga ctggatacag taaagaaaat cagcagtgaa gtaatggaag
     3301 aaaaagaaaa agaactctac atgaaattac tggaaaagaa ttgaagtaat tttctttgct
     3361 gctttgccat ttccaaattg agtaaaggat tttgttttta gctgtcccct tttcagaatt
     3421 ttcatgatag tattcatgct agagtttgtt attacattca acagaatatt atgtttgcca
     3481 tcaagagttt caatccattc agtacttgcc ctcaatgtga cacatggggt gtttaaccaa
     3541 taggcctcct tttgtaagcc tcctgaatct gttattagta aatttgcatc gcataataat
     3601 cgaagcatct cagaatattt tactggattt attgtgttta tattatttag atttaatcca
     3661 aatgatttga tttgtttttg agtgtgtgga tgtattggaa atataacatc ccatcctttt
     3721 gcagagattt gtttagcaag ttcgataatt tttttgattt tttgatagtt ttggatattg
     3781 tcttttctat gcaaggtcat tagtacatat ttgtcactat cagtttttgt gagattaagt
     3841 atttttttga attcaagaaa cacatcgtac attgtgtcac ctgtaaaata tgtagaacct
     3901 aaaactgatt cttttttgag atttttgtag cagttatggg ttggagcaaa taatatcttt
     3961 gagcaatgat cgattaatct tctattgatc tcttcttcca tatcaaattc catgcttctt
     4021 gcaccagctt ccacatgtgc aaccttcata ccaagtctgt tggcgcaaag tgctgcaccc
     4081 aacgcagatc tagtatctcc aggaactagc acaacatcgg gatcaatttt ttgtaatggt
     4141 ttttctagtt tttgaatgat ttgcgcaagc tgacctgatg gtgtacctgg tcctacttct
     4201 aaattgaaat ctggatctct tatcttcatt tcttcaaaga agactttgga catttggtag
     4261 tcgtaatgtt gacctgtatg aattatgcta agttttgatt tgtgtgataa aatctcttta
     4321 attatgggtt gagattttat gatttgaggt cgcgtaccta caataataca caatttcata
     4381 atatcaatta accagatatc agccaataat taacatttgt aaaaaattcc aaatcctttt
     4441 agtaaattat atgtcaagac atgtaagatt ggaacaaatg aatgatgatt atgtaagaat
     4501 aaagaacaag atcatgtcgt ctgaccctag tagatattgg ggtgatgatt ttgatgttag
     4561 attctatctt gtatcaaagt tgaagaaact gtataataaa tcagttctcg atgtaggagg
     4621 cggaataggc ataataagtt cggaattaga caaaagcaat tttcgaataa atgtagattc
     4681 atctttttgt gatctaaaaa catgtagaaa gaaaatggat caagatattc agaacatctg
     4741 tgcttcaatg acacacctgc cttttattga taattcattt gattctgtga tttgttccca
     4801 tgtattagaa atagcaaaac attttgatat tgaaaaaaat cagatgatta tagataacag
     4861 cattatacaa tatccaactg tagaaacgac tctcaaagag atctcgcgtg tcttaaattc
     4921 aggtggtaaa ctttttctca ctaccccaaa caacacatac tacaaatcca caaaactaaa
     4981 ttatcatgaa ttaaagagat caatatcaaa ttatttcgca agatattcgt tatcatttta
     5041 taatacattt cctcgtttaa gcagaaaaca tagaaaattg aatttggcaa atattttacc
     5101 aaagttgatg tccaatgtta tttctaagaa caaattaata acaacctcat tgattaaaga
     5161 agacagggga accgaaagag aaagtgtttc tttttattta gaagcctata aaaattaatt
     5221 ttctagaatt ttttgaagat ctgctgcaat tttattccat ccaaagtttg ttcggacata
     5281 atttgcaaaa tccttcccca ttttttcacg caattcttgt gaatttaata aagtgatcat
     5341 gtgctgtgca aattctgatg agttgcctgg acttgctaaa agaccaaaag taccatgctt
     5401 tacaattttt tctgtctctg aaacacgcgt agctacgact ggtttttcac atgcgccata
     5461 ttccagcaat ttgattggat gtgcagcagc atagtacggt tcatccggaa aggaatctaa
     5521 acacacatct gcacacagga gatattttgg gatttcctca tgtggcttcc tacctagaaa
     5581 ctcaaagtat tgacttaatc ctttcttttt gactttctct tcccatatac tttttgaacg
     5641 actaggatct ctatgatcac caattatgat atatttgatg ccactgataa cttctaccac
     5701 ttgagttgcg gaatcaatta ttatatttag atcctgtaaa gagccagaaa atatagccaa
     5761 cttatttgtt ccaaaagaat tttttaattg tttaatatca ttttgatcat gctttgatgg
     5821 attaaataat tctagatcaa ctccgtttgg cacaacatat actttggttg aatcagtttt
     5881 ttttcttgct agttcatttc tcaaatattc tgttattgct aaaatcttgt cagccatctt
     5941 aaaagaagaa tccgcaattc tctttggctt gacaaaaaca tgtaaatctg catagtctag
     6001 aataaaacga attttatgct ttgatttcat tctcatggca ggaagagaac ttcctatgcc
     6061 tggcatgtaa gagtaaacat aatcgatatg atttttttgt ataatttctg aagcggtttt
     6121 agatattgcg ggtagacatg caagataatt cagatatcgt cccaagcctt tttttccttt
     6181 gaattttaga ctttttggag gatgaatctc tgttacatca agtccatttt ctttaaatga
     6241 tgtaattcta ggagttttgt gttctggtct aattattatt ataaacacat tattaccaag
     6301 ttgtgaaaat gctttcatga gtgaaaaaac tcgagtatag tcaccccact tctgagaaag
     6361 atccacgtta caaaatacaa gtatgttcat tcaaatcacc tattcaaaaa ccactggtat
     6421 gttttcgcaa gtcctgtttc aatatcagta gttgggttgt aatgaagcat cttttgtgct
     6481 ttagaaatat ccattttacg tcttttaata tgatcaaagc ttcgcatggg tttgaaaaca
     6541 attttggatc tactttttgt aatttttaat atcatatcag caatttttct aacaggagtt
     6601 tcttttccag taccaaggtt gaaagtttca ttttttcctc gtttttgtgt tgctgcaaga
     6661 attataccgt ttacgcaatc atctacatat gtaaagtctc tagtttcgtt tcctgtacca
     6721 gttataatca ggtttttacc tctaactgct gcatgcatga aatttggaat cacgtttctg
     6781 tattttccgg gcggatcaaa tgctccgtat gaatttgata aacgaactat agtgcaatct
     6841 attccgtgta gttcatgaaa ggtacgacag tagtgctctg cagtaagctt actggctgca
     6901 taaggcgtag aaggatggag aatcaagctt tctttgagtt ttgttgattc ttgtggtcca
     6961 taaatagacg atgatgatgt atacaatagt ctttcaggtt tttgtttaga ggcgtgcatc
     7021 aagatttgta gagttccttg aacatttaca cgtagatcct ccagtgggtc tttaatggaa
     7081 agctcatttg caaatcttgc tgctaaatga aaaacataat caaattgatg tgaaaaaact
     7141 ttggaaagta tttttttgtt tgtgatatcg cctttgatta cacaaagatt tctgtttgaa
     7201 ggcaaattac cagtcttgct ttgtgaaaaa tcatccaaga caacaacttt ggaattcata
     7261 tttagcaatt ttctcacaag atttgaacca attgctccat ttccgccagt cacaagtata
     7321 gaggcaccac taaaattctt catgtcagag tctttttatc ttgcttcatt tatgtttgag
     7381 gttttttgta tcctatagaa agataatgga ttccaagatc ttctacttct tttctttgaa
     7441 agattcttct tgtatcaaca agtatgggag ttttcttcat tttttggatc atcttaaggt
     7501 ctagtttttt aaactcatta tgatctgtta ctactatcat agcatcacaa gaagatatta
     7561 cattttggat gtctgtaatt ttttgtccac caaagctttc ttccgttttt ggatcaaaga
     7621 caagtacctt acatccatcc ttgagaagat atgatatgat ttcttttgcg ggagaaaatc
     7681 ttgtatcact tacatttccc ttgtatgcaa ttccaagaac caagatagta gattcagcaa
     7741 tctttttgtt ttgtgccttt aatgcatctg ttacaagatt tacaacatga tatggcatac
     7801 tgtcattaat tttacgtgat tcaagaatta tcttagaatc aatctgtgag tttccttgtg
     7861 ggtttagtaa taggtatgga tctttaggaa gacatggtcc acctacacct ggacctggtt
     7921 gaagtagttt tactcttggg tgcgagttac aaaccctgat gagttctgca acatctacac
     7981 catatttttc acaaatcatt cctacttcat ttgcaaatgc cacgtttaca tcacgaaaag
     8041 tgttttcaac tagctttgag atctctgcaa tttgagcagt tgttagaaat atttgtgatt
     8101 tgactatttt ttcgtataat gtttttgcaa gtagcccaga atcttcatcc acatagccga
     8161 tcactcgtgg tgtagtctgt atttcagata atgcttgacc aggtgctagt ctctcaggaa
     8221 caaaagctgc ccagaagtca gaaccaattc tgtattttgt tccgagagaa ggcataacat
     8281 atcctgcaaa tgtacctggt ggaattgagc tctcaattat cacaagtttg tttgccagag
     8341 atagtccaga aagtgatttg catacattct caagagcaga tagatctggt cttacaccat
     8401 ttgtaagcgg agtagcaaca cagattataa taacatcaga attctttaca gattctgcaa
     8461 gattattgtt gatatgaaac ttatctttat taattgagga ttttagtaaa tcatcaagat
     8521 catcttcttc aaatgggagt ttttttgaat ttagtgttga taagagtttt tcatttatat
     8581 catgacctat aacatcaaat cctgatttac aaaaagtcaa tgcagttgga agaccaacct
     8641 gtcca
//
